LOCUS       DATAIW010000001         7566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_119168, whole genome shotgun sequence.
ACCESSION   DATAIW010000001 DATAIW010000000
VERSION     DATAIW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119168"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHM48_00005"
     CDS             <1..485
                     /locus_tag="VHM48_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753810.1"
                     /translation="TGPMAGLALEAAHRRIPDGPAEQPRGAPPEGAVGADRRDVGGPI
                     ADAPASEPAGRGPGLNPTGIGAMANCTAPQLRRFIKSRPYVPMHELRRRFGINGSEDD
                     VSSIELGDHHRVYVGLPPREGSLLGELLRAGDIGYELSLDPRTPVVIGLYPMRPVARS
                     "
     gene            756..1733
                     /locus_tag="VHM48_00010"
     CDS             756..1733
                     /locus_tag="VHM48_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753811.1"
                     /translation="MRATSMRRLIGGAAALMLVGTTLLTAAGVALAADTRKIYIGPDP
                     AFTDGNATLVFTPVTAGGDSLSTIYVKNIDNQTLTHVVITIAKSQDGSTISDQVLGPN
                     ASKCDSSIDPITCDFGNLKANATRQFSIIIRAGTGTTSFVTAKVVFNESNNPNGGNQQ
                     IESADGTLPIGAATCHSLATFLPPGIGKTLIPDDGTTCSGEGQRSGLIVPSNANGNIV
                     SVNDATTASGCPTGFSCLGNQVLGTVNNGSTVSPFLKWSIFYSNAVLGTVNPGKVAFV
                     HEGTTILAGNKGLCKTPNSSNCHEPYQVQAGGVLFIIRTDTNGLIKGMH"
     gene            complement(1748..5197)
                     /locus_tag="VHM48_00015"
     CDS             complement(1748..5197)
                     /locus_tag="VHM48_00015"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2753812.1"
                     /translation="MDTVACPSCGEENPARFRLCGYCGSSLAPVPETIVCSGCGEENP
                     SKFRLCGFCGTPLAAGAAADGSERGSAPAAPHPPITPTRPATDPGPHLGVSLAATASG
                     DASPFGSLLAPTRPRPASTAPILPAQEVRKFVTLVFTDLKDSTALTGSIDAEAMNEIK
                     ARYFSSMAVEIERHGGKVEKNIGDAIMAVFGLIRAHEDDALRAVRAAYGMQKVLAALN
                     EDLQTFYGVQITNRTGVNTGEIVANTDPAADQNLATGDAVNVTARLEQNAPAGEILIG
                     EVTYELVRDHVEVERLELMLKGKPEPVPAYRLIEVRAEPVARLGAAAAPFVGREGEMD
                     ILRGAFAEASTTHSARLVTVIGDAGVGKTRLISDFVGRVEGEATVHRGRCLAYGDGIT
                     FWPLIEIVRSAARIGEDDSPETARARITSLLPTDDPDRDAIVDRVASAVGLSSSSHPV
                     AELFWGARKLLESQAIERPLVIVIDDIHSAEATFLEFLDHLVESVQEAAILILCSARP
                     ELVDVHGDWLASAGVERIDLAPLGAADVEAMIDRLLAGVALSAETRGKVVVAAEGNPL
                     YIEQIVSLLRERDPGGDVVVPPTIAALLAERLDGLSREERAVVDPAAVIGLVFPAAAI
                     AQLVPDQIRLEVDDHLVALDRKQFVHPLTADGDDAAFRFHHILVRDAAYQSLLKRARA
                     TLHERFVEWAEPVNRDRGRETEFEEILGYHLEQAVRYRSELGPLDDQGRSIGRRAATK
                     LSSAGRRAFGRGDTPAACNLLRRAVALLPTDDPERVELIGDLADALMEEGHFDEARDV
                     IAEGAAIAERLADRRLAARVRLAARRLDLVLSELEGSQSAIADTTEAIEILAAAGDEA
                     GLARAWRLLMIIHGTTGAYDDAAEAAKQVVEHARAAGDTRQAARGAMGYATTALLGPT
                     PVSEALATCEGLVRDVSGDRKAESVILGVLAQLHAMNGEFDIARDLYRRAAASLADLG
                     PSITSATLSTESSRVESLAGDYPAAERELRRDDLALAAMGERYYRSTVDALLAQVLVV
                     QGNLDEADAFSRQAEELADDDDVSSQVFWRSARARIYAGTGRPDEAEALARQAVEMAR
                     GTVDICLLGGALLDLADVVAQAGRRDEAEPPMREALQLFERKEDRTSALRVRGLLGEP
                     AAV"
     gene            5293..5994
                     /locus_tag="VHM48_00020"
     CDS             5293..5994
                     /locus_tag="VHM48_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2753813.1"
                     /translation="MAVGDDAMVRGALKASGLFGAADEAAIDALIRVLRIRRFRRGET
                     IFHQGDPGDALFVLASGSVKVVLPSDEGAEPAIVAILGPGEFFGELAILDGAPHSATI
                     VAVEATETLVLHRDAFLSLIDSDPGLRRALLASLATEIRRLTGHVEDLHFLDLPGRLA
                     SRILRLAASAGATTGEARIAWPYTQSELAGMIGGSRQSVNRLLADLTDQGLVRLERDH
                     LVVGDVERLARTVER"
     gene            5991..6782
                     /locus_tag="VHM48_00025"
     CDS             5991..6782
                     /locus_tag="VHM48_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2753814.1"
                     /translation="MTDDPDPLIPTLHAVALRVEAARRLAPPAGTAVLRSIVEATAVL
                     FGAEAASLALHDPATGRLVFAVAAGEHGQGVVGLAIGSSEGVAGYVFSTGQPLALSDV
                     ARDARFGREAAEQTGYVPRSLIAVPLVDDEGILGVLEVLDKRGEATFDLRDVELATVF
                     ARQATVAIRSSRIERDTASLLRAVLVAADAAPGAAEGGIEDALDSMIAAEIRRLDADA
                     DDPLWALADQIALLRAADPGQLELVRDLLAVLVRRADRGRGGRGR"
     gene            6779..>7566
                     /locus_tag="VHM48_00030"
     CDS             6779..>7566
                     /locus_tag="VHM48_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2753815.1"
                     /translation="MSQSLPAWSEPFSIDHRSALRRLAPFEAIDRTWAFGAGDGEGVV
                     VAIVDSGVEGGHPAIAGRLRESVRVELDGDDATVVVDQPTDAVGHGTACAGIILGLAP
                     RTTVLSVRVLGSDNRGKGLAFATGLQWAIEHGASIVNLSLSSRSESLYATLHDLVDRA
                     YFANVLLVCAANNVAVASYPSLFASVVSVAAHDVRDPDVWYYNPRPPVEFGAYGLDVD
                     VAWKDGGRITATGNSFAAPHIAAYAARIRAAHPTATPFEIKTILA"
BASE COUNT         1106 a         2680 c         2660 g         1120 t
ORIGIN      
        1 ccaccgggcc gatggcgggc ctcgcgctgg aggcggctca ccggcgaatt cccgacggcc
       61 cggcggaaca gcctcgcggc gcgccgccgg aaggcgcggt cggggccgac cgccgcgacg
      121 tcggtggccc gattgccgac gcgcccgcgt cggaaccggc cggacgcggc ccgggcctga
      181 acccgaccgg tatcggagcg atggccaact gcacggcgcc ccagctccgc cggttcatca
      241 agagccgccc ctatgtgccg atgcacgagc tgcgacgccg gttcgggatc aacggcagcg
      301 aggacgatgt ctcctcgatc gagcttggcg atcaccatcg ggtgtacgtc ggcttgcccc
      361 cgcgcgaggg gtcgctcctc ggcgagctgc tgcgggccgg cgacatcggc tatgagctgt
      421 cgctcgaccc caggaccccg gtcgtgatcg ggctgtatcc gatgcggccc gtcgcccgct
      481 cctgagctcg accggagcct tctccgccga ggcaggcgca cgtgccgcaa caggccggaa
      541 catcgtcgac cgatcacgaa cgaagtgttg acagcgcgca ccgtcggcat cttgacacca
      601 cgcgtgtcgg atgtgaccgt tgaccccgga cgttcgtacc agatactggc ctgaccattg
      661 gggtcgacgg ttccaggcca ggcgtccaac tcacgcattg ggccggaggg gccgctggac
      721 gtttccggag tgcgctccgg cgaaagggat cacgaatgcg cgcgacatcg atgcggaggc
      781 tgatcggggg agctgccgcg ctcatgctcg tcgggacgac gctcctgacc gccgccgggg
      841 tcgccctggc cgcggacacc cggaagatct acatcgggcc cgatccggcc ttcaccgacg
      901 gaaacgcaac gcttgtgttc acgccggtca cggccggcgg tgacagcctg tcgacgatct
      961 acgtcaagaa catcgacaac cagaccctga cccatgtcgt gatcacgatc gcgaagagcc
     1021 aggacggctc gacgatctcc gaccaggtcc tcggaccgaa cgcgtccaaa tgcgatagct
     1081 cgattgatcc gatcacgtgc gacttcggga acctcaaggc gaacgccacg cgccagttct
     1141 cgatcatcat ccgcgcagga acaggcacga ccagtttcgt cacggccaag gtcgtcttca
     1201 atgagtccaa caatccgaac ggcggcaacc agcagatcga gtcagcggac ggaacgctcc
     1261 cgattggcgc cgcaacatgc cacagcctcg cgaccttcct tccgcccggc atcgggaaga
     1321 cgctgatccc ggatgacggc accacctgct cgggtgaggg tcagcggagc gggctgatcg
     1381 ttccttccaa cgccaacggc aacatcgtct cggtgaacga cgcgaccacg gccagcggct
     1441 gccccacggg cttctcgtgc ctcggcaacc aggtcctcgg gacggtcaac aacggctcca
     1501 ccgtctcgcc cttcctgaag tggtcgatct tctactcgaa cgctgtgctc ggcaccgtca
     1561 accccggcaa ggttgccttc gtccacgagg gcacgaccat cctggccggc aacaagggtc
     1621 tgtgcaagac cccgaactca tcgaattgcc acgagccgta ccaggtccag gcaggtggcg
     1681 ttctgttcat catccgaacc gacacgaacg gcctgatcaa gggcatgcac tgatcggcgc
     1741 gtaaccctca gaccgcggcc ggctcgccga gcaggccgcg gacccggagg gcggacgtcc
     1801 ggtcctcctt tcgctcgaac agctgcaggg cctcccgcat gggaggctct gcttcgtctc
     1861 ggcgacccgc ctgagccacg acgtccgcga gatcgagcag cgcgccgccg agcaggcaga
     1921 tgtcgaccgt gccgcgtgcc atctcgaccg cctgacgggc aagggcctca gcctcgtcgg
     1981 gtcgcccggt cccggcatag atccgggccc gggcggagcg ccagaagacc tgcgatgaca
     2041 cgtcgtcgtc gtcggccagc tcctcggcct ggcggctgaa ggcatcagct tcgtcgaggt
     2101 tgccctgcac gacgaggacc tgggcgagga gggcatcgac ggtcgagcgg taatagcgct
     2161 cgcccatcgc cgccagggcc aggtcgtcgc ggcgcagctc ccgctcggcg gccgggtagt
     2221 caccggccag cgactcgacc cgtgacgatt ccgtcgacag ggtcgccgag gtgatgctcg
     2281 gtccgaggtc ggcgagcgac gccgcggcgc gccggtacag gtcacgggcg atgtcgaact
     2341 cgccgttcat cgcgtggagc tgggcaagga cgccgaggat caccgactcg gcctttcgat
     2401 cgccgctgac atcgcggacc aggccctcgc acgtggcaag ggcctcgctc acgggcgtcg
     2461 ggccgagcag ggcggtcgtg gcgtagccca tcgcgccgcg cgccgcctgg cgcgtgtcgc
     2521 ccgcggctcg ggcgtgctcg acgacctgct tggccgcctc ggcggcgtcg tcgtaggcgc
     2581 cggtcgttcc atggatgatc atcagcaatc gccacgcccg ggcgaggccg gcctcgtcgc
     2641 cggccgcggc caggatctcg atcgcctcgg tcgtgtcggc gatcgcgctc tggctgcctt
     2701 cgagctcgga caggacgagg tcgagccgcc gtgcggcaag ccggacgcgc gccgccagcc
     2761 gccgatcggc gaggcgctcg gcgatcgcgg cgccctcggc gatcacgtcc cgcgcctcgt
     2821 cgaagtgccc ctcctccatg agtgcgtcgg ccaggtcacc gatcagctcg acccgctccg
     2881 ggtcgtcggt cggcaagagg gcgacggccc gacgcagcag gttgcaggcg gccggcgtat
     2941 cgccgcgccc gaacgcacgc cgcccggcag agctgagctt ggtcgccgct cgccgaccga
     3001 tcgagcggcc ctggtcgtcg aggggcccga gctcagagcg atagcggaca gcctgttcga
     3061 ggtgatagcc gaggatctcc tcgaactcgg tctcgcggcc gcggtctcgg ttgaccggct
     3121 cggcccactc gacgaaccgc tcgtggagcg tcgcccgggc ccgtttgagc aggctctggt
     3181 aggcggcgtc gcggacgagg atgtggtgga agcggaacgc ggcgtcgtcg ccatcggcgg
     3241 tcagcggatg gacgaactgc ttgcggtcga gggccaccag gtggtcgtcg acctcgagcc
     3301 ggatctggtc ggggacgagc tgggcgatcg ccgcggccgg gaagaccagg ccgatgaccg
     3361 cagccggatc gacgacggcc cgctcctcgc gcgagaggcc gtccagtcgt tcggcgagca
     3421 gggccgcgat cgtcggcggg acgacgacgt cgccgccggg atcgcgctcg cggaggaggc
     3481 tgacgatctg ttcgatgtac agcggattgc cctcggcggc gacgacgacc ttgccgcgcg
     3541 tctccgccga cagcgccacg ccggcgagga ggcgatcgat catggcctcg acatctgccg
     3601 cgccgagcgg cgcgaggtcg atccgttcga ctccggccga ggcgagccag tcgccgtgga
     3661 cgtcgaccag ctccggccgg gccgagcaga ggatgaggat cgcggcttcc tgcaccgatt
     3721 cgacgaggtg atcgaggaac tcgaggaagg tcgcctcagc cgaatggatg tcgtcgatga
     3781 cgatgacgag cggccgctcg atcgcctgcg actcgagcag cttccgggcg ccccagaaca
     3841 gctcggcaac ggggtgcgac gaactggaca ggccgacggc cgatgcgaca cggtcgacga
     3901 tcgcgtcgcg gtccgggtcg tcggtcggca ggagggaggt gatccgcgcc cgggcggtct
     3961 cgggcgagtc gtcctcgcca atccgtgcgg ccgatcggac gatctcgatg agcggccaga
     4021 acgtgatccc gtcgccgtac gcgaggcagc gcccgcggtg gacggtcgcc tcgccttcca
     4081 cccggccgac gaagtcggag atcagcctgg tcttgccgac accagcatcg ccgatgacgg
     4141 tcacgagtcg cgccgagtgg gtcgtcgagg cctcggcgaa cgccccgcgc aggatatcca
     4201 tctcgccttc gcgaccgacg aacggcgcgg ccgcggcgcc gaggcgcgca accggctccg
     4261 cccggacctc gatcaggcga taggccggga ccggctcggg cttgcccttg agcatcagct
     4321 cgagccgttc gacctcgaca tggtcgcgga cgagctcgta ggtgacctcg ccgatcagga
     4381 tctcgccggc gggagcgttc tgctcgaggc gggcggtgac gttgaccgcg tcgccggtcg
     4441 cgagattctg gtcggctgcc ggatccgtgt tggcgacgat ctcaccggtg ttgaccccgg
     4501 tccggttggt gatctggacg ccgtagaacg tctgcaggtc ctcgttcagc gcggccagca
     4561 ccttctgcat cccgtacgcc gcccggacgg cgcgcagggc gtcgtcctcg tgggcccgga
     4621 tcaggccgaa gacggccatg atcgcgtcgc cgatgttctt ctcgaccttg ccgccgtggc
     4681 gctcgatctc gaccgccatc gagctgaagt agcgggcctt gatctcgttc atcgcctcgg
     4741 cgtcgatcga gcccgtcagc gccgtggagt ccttgagatc ggtgaagacg agggtgacga
     4801 acttgcgcac ttcctgggcc ggcaggatcg gcgccgtcga ggccggtcgc ggccgcgtag
     4861 gcgcgaggag gctgccgaac gggctcgcgt cgccgctcgc cgtggcggca agcgagacgc
     4921 ccaagtgcgg acccgggtcg gtggccggac gggtcggcgt gatcggagga tgcggggccg
     4981 ccggcgccga tccgcgctcc gatccgtctg ctgccgcgcc ggccgcgagc ggcgtcccgc
     5041 agaacccgca gaggcggaac ttcgaggggt tctcctcgcc gcagccgctg cagacgatcg
     5101 tctcgggcac gggcgcaagg gagcttccgc agtacccgca gaggcggaag cgggccgggt
     5161 tctcctcgcc gcaggaaggg caggccaccg tgtccatggc gggaggatac gacggcgcgc
     5221 gacgatcgcc gcctcgcacc gcgtacgatg cgaccgagcc gcatgcgtct gcggccgcgc
     5281 aggaggtccg ggatggcggt cggcgacgac gcgatggtaa ggggggcgct caaggcgagc
     5341 gggctcttcg gggccgccga cgaggccgcc atcgacgcgt tgatccgtgt cctccggatc
     5401 cggcggttcc ggcgaggcga gaccatcttc caccagggcg accctggcga tgccctcttc
     5461 gtcctggcca gcggctcggt caaggtcgtc ctgccgagcg acgagggtgc cgagccggcg
     5521 atcgtggcga tcctgggacc cggcgaattc ttcggcgagc tggcgatcct cgacggggcg
     5581 ccgcactcgg ccacgatcgt ggcggtcgag gcgacggaga ccctcgtcct ccatcgcgat
     5641 gccttcctca gcctgatcga ttccgatccc ggcctgcgtc gagcgctcct cgcctcgctg
     5701 gccacggaga tccggcgcct caccggccat gtcgaggacc tccacttcct cgacctgccg
     5761 ggacgcctcg cctcgcggat cctccgtctg gcggcgagcg ccggggcaac gaccggcgag
     5821 gcccggatcg cctggcccta cacgcagtcg gagctggccg gaatgatcgg cggctcgcgc
     5881 caatcggtga atcgcctcct cgccgacctg acagaccagg ggctcgtccg gctcgagcgc
     5941 gaccatctcg tcgtcggtga tgtcgagcgg ctcgcccgaa cggtcgagcg gtgaccgacg
     6001 atccggaccc gctgatcccg accctccatg ctgtggccct ccgggtcgag gcggctcgcc
     6061 gtctcgcgcc gccggccggg acggcggttc tccgctcgat cgtcgaggcg accgccgtcc
     6121 tgttcggggc cgaggcggcg tcgctggcgc tccatgaccc cgccacgggc cgcctcgtgt
     6181 tcgcggtcgc cgccggcgag cacgggcagg gcgtcgtcgg gttggcgatc ggatcgagcg
     6241 agggtgtcgc gggctacgtg ttctcgacgg gccagccgct cgctctgtcg gatgtcgctc
     6301 gcgatgcccg gttcggccgc gaggcggcgg aacagaccgg ctacgtcccg cgatcgctga
     6361 tcgccgttcc gctcgtcgac gacgaaggga tcctcggcgt tctcgaggtc ctcgacaagc
     6421 gcggcgaggc cacgttcgac ctccgcgatg tcgagctcgc gaccgtgttc gcgcgccagg
     6481 cgaccgtggc catccggagc agccggatcg agcgcgacac ggcgagcctg ctgcgggcgg
     6541 tcctcgttgc ggctgacgcg gcgcccggcg cagccgaagg aggcatcgag gacgccctcg
     6601 attcgatgat cgcggcggag atccgcaggc tcgacgccga cgccgacgat ccgctgtggg
     6661 ccctggccga ccagatcgcc ctgctccggg ccgccgaccc cggccagctc gagctcgtgc
     6721 gcgacctgct ggccgtcctc gtccggcgcg cggaccgcgg tcggggtggc cgcggccgat
     6781 gagccagtcc ctgcccgcct ggagcgagcc gttctccatc gatcaccggt ccgccctgcg
     6841 ccgcctggct cccttcgagg cgatcgaccg gacctgggca ttcggtgctg gcgacggcga
     6901 gggcgtggtc gtcgcgatcg tcgactcggg cgtcgagggt gggcatccgg cgatcgccgg
     6961 acggctccgc gaaagcgtcc gggtcgagct cgacggggac gacgccacgg tcgtggtcga
     7021 ccagccgacc gacgccgtcg gccacgggac cgcctgcgcc gggatcatcc tcgggctcgc
     7081 cccacggacg accgtcctgt ccgtccgcgt ccttggatcg gacaaccggg gcaagggcct
     7141 ggcattcgcg acgggcctcc aatgggcgat cgaacatggc gcgtcgatcg tgaacctcag
     7201 cctctcgtcg cggagcgagt cgctctacgc gaccctccac gatctcgtcg accgggccta
     7261 tttcgccaac gtcctgctcg tctgcgcggc caacaacgtg gcggtcgcga gctacccatc
     7321 cctgttcgcg tccgtcgtgt cggttgccgc gcacgacgtt cgcgatccgg acgtctggta
     7381 ttacaacccg cggcccccgg tcgagttcgg ggcgtacggg ctcgacgtcg acgtcgcctg
     7441 gaaggacggc ggccggatca cggccacggg caacagcttc gccgctccgc acatcgccgc
     7501 gtacgcggcc cgcatccgcg cggcccaccc gaccgcgacg ccgttcgaga tcaagacgat
     7561 cctcgc
//
LOCUS       DATAIW010000002         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_106, whole genome shotgun sequence.
ACCESSION   DATAIW010000002 DATAIW010000000
VERSION     DATAIW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..452)
                     /locus_tag="VHM48_00035"
     CDS             complement(27..452)
                     /locus_tag="VHM48_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753816.1"
                     /translation="MTPRSSLRPLDVVIRIAIVGLTLGTAYIHSTLGGLLFTLNALGY
                     LVAAVAVVIPLGLAIRFRWSVRLGLIGYAATAIVAWAVQGPYYSTAFIAKGIEVALIT
                     LLAIDFARMDGNPVEVVKSELALLASKLGRRPATGQAGA"
     gene            710..>2610
                     /locus_tag="VHM48_00040"
     CDS             710..>2610
                     /locus_tag="VHM48_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2753817.1"
                     /translation="MATTRPRRRAPEPPATAVDRDDSRLEDDDRPSGADLISAENRAT
                     LHHALEEAARLLRSDGAMAYLLDPETGILRFADDAGITDDRRRRWVRALKVEPGVGLF
                     GQAVADRRIHHTGDYPTDPSFVHFPGADRLVRTLAIHSFLVAPLIAGDHVFGAMGTYS
                     SRPDSFDEHDVALVRALADHAAAAMANAELIEQLAGSRAELERRADAERSLREIGARI
                     TAVRDPAEVLQLAVDEAARLLGADGARIDLLDDDGALYWAYDATTGRRPGLGPIGGSG
                     EAKAGEGISGRAVREMQAVFTGDYLHDERFEHAALPDAHVRKFAIRSVVAVPLVRDGT
                     PLGTLTVYTGEVDAFGEADARLLEALAGQAAIALTNAGLIERLAAAQADERRRADEER
                     ALREIAARITAIRDPSELLQDVVDETARLLGAERVRIDLIGRGAGRVGYTFMGAGPHI
                     GGAAVDEDGEPYLVGASGQAIVERRTVVVGDYLSDDSFDHVPELDRAVAEDGIRSLVI
                     TPLIAEDSLLGVLQVGSMEIDAFGLDQVGLAEALAHQAAIAIHNSGLIQALERSAVQI
                     RRRAAAEQALREIATRITAIRDPTELLQQVTDSGRRLLDGDRAQLDIVDPATGLIHWS
                     RVSGDGPFGG"
BASE COUNT          362 a          947 c          938 g          363 t
ORIGIN      
        1 cagccgggtc ccgcgggaac gatgggtcag gcgcctgcct ggccggtcgc cgggcggcgg
       61 ccaagcttcg aggcgaggag cgcgagctcg ctcttgacga cctcgacggg gttgccgtcc
      121 atccgggcga agtcgatcgc gagcagggtg atgagcgcga cctcgatgcc cttggcgatg
      181 aaggccgtcg agtagtaggg accctggacg gcccaggcca cgatcgccgt cgccgcgtag
      241 ccgatcaggc cgaggcggac ggaccagcgg aagcggatgg ccagcccgag cggtatgacg
      301 accgcgaccg cggcgacgag atagccgagt gcattcaggg tgaagagcag gccgcccagg
      361 gtcgagtgga tgtaggccgt gccgagggtc aggcccacga tcgcgatccg gatgaccacg
      421 tcgagagggc gcaggctgct gcggggagtc atgtcgtcgg gtacctcagg gtgatgggtt
      481 gcgtcgattc gccgcgcttc gtcggcgtcg ggtcgcatcg tcgccgctcg cggccccgac
      541 cggcgtcggc tccggtccac aacggcatgc gtcgttggca gggacgtccg ggtggtcgcc
      601 ggtccgtggg cgatgccgtc atccggccag cgcggccgcg acggatgacg taaaccgcct
      661 catccgcggt cgatgccccg gtggcgggat cgcgtcgtat cctcccgacg tggccacgac
      721 ccgtccgcgc cgccgcgcac ccgaaccgcc tgccacggcg gtggatcgcg acgattcgcg
      781 cctcgaggat gacgatcgcc cgtcgggcgc cgacctcatc agcgccgaga accgggcgac
      841 cctccaccat gccctcgaag aggccgcccg cctgctccgc tccgatggtg cgatggccta
      901 cctgctggat ccggagaccg ggatcctccg cttcgccgat gacgccggga tcaccgacga
      961 tcgccgccgt cgctgggtcc gggccctgaa ggtcgagccg ggggtcggcc tgttcgggca
     1021 ggcggtcgcg gatcgccgga tccaccacac cggcgactac cccaccgacc ccagcttcgt
     1081 ccatttcccg ggtgccgacc ggttggtgcg gaccctcgcc atccactcgt tcctcgtcgc
     1141 cccgctgatc gccggcgacc acgtattcgg agcgatgggc acctattcca gccggcccga
     1201 ctcgttcgac gagcacgatg tcgcgctcgt ccgggcgttg gccgaccatg ccgctgcggc
     1261 catggccaac gccgagctga tcgagcaact cgccggctcc cgggcggagc tcgagcggcg
     1321 ggcggacgcc gagcgctccc tccgtgagat cggcgcccgg atcaccgctg tccgcgatcc
     1381 cgccgaggtc ctccagctcg cggtcgacga ggcggcccgg ctgcttggcg cggacggcgc
     1441 acggatcgac ctcctcgacg acgacggcgc gctgtattgg gcctacgacg ccacgaccgg
     1501 ccgccggccg ggtctcgggc cgatcggcgg gagcggcgag gcgaaggccg gcgagggcat
     1561 ctctggtcgg gccgtccgcg agatgcaggc cgtcttcacg ggggactacc tccacgacga
     1621 gcgcttcgaa catgcggcac tgccggatgc gcacgtcagg aagttcgcga tccgctcggt
     1681 cgtggcggtc ccgctcgtcc gcgacggcac gccgctcggg acgctgaccg tctacaccgg
     1741 cgaggtcgac gccttcggcg aagcggatgc ccgcctgctg gaggcgctcg ccggccaggc
     1801 ggcgatcgcg ctgacgaacg ccggcctgat cgagcggctc gcggccgccc aggccgatga
     1861 gcggcggcgc gccgacgagg agcgcgcact tcgcgagatc gcggcgcgca tcaccgcgat
     1921 ccgcgaccca tccgagctcc tccaggacgt cgtcgacgag acggcgcgcc tcctcggggc
     1981 ggagcgcgtc cggatcgacc tgatcggccg tggcgccggt cgtgtcggct acacgttcat
     2041 gggcgccggt ccgcacatcg gtggggcggc ggtcgacgag gacggcgagc cctacctcgt
     2101 cggcgcatcc ggccaggcga tcgtcgagcg gcgcacggtc gtcgtcggtg actacctcag
     2161 cgacgactcc ttcgaccacg tcccggagct cgaccgagcg gtcgccgagg acgggatccg
     2221 ctccctcgtg atcacgcccc tgatcgccga agactccctg ctcggcgtcc tccaggtcgg
     2281 cagcatggag atcgacgcgt tcgggctcga ccaggtcggg ctggccgagg cgctggccca
     2341 ccaggccgcg atcgcgatcc acaactcggg gctgatccag gccctcgagc ggtcggccgt
     2401 gcagatccgc cggcgggctg ccgccgagca ggccctccgc gagatcgcga cccggatcac
     2461 cgcaatccgc gacccgaccg agctcctcca gcaggtgacc gattccggcc gccgcctgct
     2521 cgacggcgac cgtgcccagc tcgacatcgt cgatcccgcg acgggcctga tccactggtc
     2581 ccgggtgtcg ggtgatgggc cattcggggg
//
LOCUS       DATAIW010000003         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_119201, whole genome shotgun sequence.
ACCESSION   DATAIW010000003 DATAIW010000000
VERSION     DATAIW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119201"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VHM48_00045"
     CDS             complement(<1..445)
                     /locus_tag="VHM48_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX2753818.1"
                     /translation="MTCRSCEVRIERNLRRLPNIERVSASAVHARVEIVSSGPVPAAD
                     IAAAIEAAGYEVGRTSWLERDAQVWATAAGGLLVLVLIAVLAQATGIAGLASGAGSLS
                     QGGVVVALLLGLAAGVSTCMALVGGVVLALSAANETRRSATRGDVG"
     gene            788..1474
                     /locus_tag="VHM48_00050"
     CDS             788..1474
                     /locus_tag="VHM48_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2753819.1"
                     /translation="MQPPRILVVDDEAPIVDLVRGYLEREGFVVSSAADGPSAVEAVR
                     TTSPDVVILDVMLPGLDGIEVCRQIRTFSNAYVLMLTARGEEIDRIMGLTVGADDYLV
                     KPFSPRELVARVKALLRRPLASAEPALATPAGLDLDSARRQVRVDGTIVDVTAMEFDL
                     LEVLVRDPGVVIGRQTLLDRVWGPEFVADDHLVDVHIGNLRKKLGDDPAAPRFIETVR
                     RVGYRLREGA"
     gene            1474..2562
                     /locus_tag="VHM48_00055"
     CDS             1474..2562
                     /locus_tag="VHM48_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2753820.1"
                     /translation="MVPRSLPLRLLAAFLVVVAASIGTVGVAVLLFGPGYFAEAMGHM
                     PGDPMGDAMGQATQAAFADAMRQAIVAATVVAIVTASVVSLAVAARIARPITALAAAA
                     RRVAGGDYAERVPVDEPGELAELATSFNDMAGSLEATERRRLQLVGDVAHELRTPLTT
                     LDGYLEGLEDGVIEPNPETWRLLRAETGRLTRLVADLSELWRAEARQLSLRLESVDLV
                     GVARDVVERFVPVAAAKSIHIAVPPTPLLALADHDRVAQVIGNYLSNAIRHAPDGSTV
                     TISAATTAGRAVLGVTDRGSGLADDQLEAVFERFYRVDPARSRSAGGSGIGLTIARAL
                     AQAMGGWAWAERPRDGIGVTFLLDLPPA"
     gene            complement(2649..3065)
                     /locus_tag="VHM48_00060"
     CDS             complement(2649..3065)
                     /locus_tag="VHM48_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753821.1"
                     /translation="MPRFALPRPTALIASGGLALAMALGSIAAPVATLASGGSAVRAV
                     GHCTGHSTSALKAKHDNGRIEVEFEVDQNRSNRLWYVRVTDDTHLVYRGSKRTVAPSG
                     SFTVRLLIPNRAGTDTIAARAVNATTGEVCTARLNV"
     gene            complement(3290..3772)
                     /locus_tag="VHM48_00065"
     CDS             complement(3290..3772)
                     /locus_tag="VHM48_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753822.1"
                     /translation="MPNAPNGGVSFADVRHGCAGMFDTPADMTNDGGGTWAPVNAAAG
                     LAACAAGLVDPVWAEPSRLFDPGNLLGVQAIVGPDRAWALGSLDLDHFGVAATADGGR
                     TWTGYRWPLPPDGVGGFGPDTLVRATFVSPTMGWVFTRFGRIYETTDAGSSWREISIG
                     "
BASE COUNT          530 a         1381 c         1469 g          568 t
ORIGIN      
        1 cccccacgtc accccgggtt gcggaccgcc gcgtctcgtt cgccgcggag agggcgagga
       61 cgacgccgcc gacgagggcc atgcacgtcg ataccccggc ggcgagcccg agcaggagcg
      121 cgacgacgac gccgccctgg ctcaggctgc ccgccccgga cgcgagcccg gcgatccccg
      181 tcgcctgggc gaggaccgcg atcaggacga ggacgagaag cccgccggcg gcggtcgccc
      241 agacctgggc gtcgcgctcg agccatgacg tccggccaac ctcgtatccg gcggcctcga
      301 tggcggcggc gatgtccgcg gccggcacgg ggccggagct gacgatctcg acccgggcgt
      361 ggaccgcgga cgcggagacc cgctcgatgt tgggcaggcg gcggaggttg cgctcgatgc
      421 ggacctcgca gctgcggcag gtcatgcccg cgatcgggac gatcgtcgag gttcgcgggc
      481 ccggatcaga ggtgaccggg cgggccggag ccgggatgac ggcggcggat cgggctgcgc
      541 ggttgcgcga gcgggtcgtt cggtgcgaca ttcgggacgc ctgatggtcg gtgcgaggcc
      601 gtgcacggcg ggcgccgggc tcgcgtccgg cacgtcgtga gcgtcggcgt tgcgggtcag
      661 gtgtcgatga agaggggcgc aagactcggt caagagttgg tcaagaccgc cggtgcgagc
      721 gagcggcgag gcggtcggtt ggctcggcgc ccggcctcac ccgaccggtc cgtgccatca
      781 tcgggccatg cagccgccgc ggatcctggt cgtcgatgac gaggcgccga tcgtcgacct
      841 cgtccgcggg tatctggagc gcgagggctt cgtcgtctcg tcggcggcgg acggcccgtc
      901 ggccgtggag gccgtgcgaa cgacctcgcc ggacgtcgtg attctcgacg tcatgctgcc
      961 gggcctcgac gggatcgagg tctgtaggca aatccggacc ttctcgaacg cgtacgtcct
     1021 gatgctgacc gcccggggcg aggagatcga ccggatcatg ggcctgaccg tcggggccga
     1081 cgactacctc gtgaagccgt tctcaccgcg cgagctggtc gcgagagtga aggccctcct
     1141 tcgacgaccg cttgcgtccg ctgagccggc gctcgccacg ccggccgggc tcgacctcga
     1201 ctctgcccgt cgccaagttc gggtcgacgg gaccatcgtc gacgtcacgg cgatggagtt
     1261 cgacctcctc gaggtcctcg tccgtgatcc cggcgtcgtc atcgggcggc agaccctgct
     1321 cgatcgcgta tggggcccgg aattcgttgc cgacgaccac ctcgtcgacg tccacatcgg
     1381 caacctccgc aagaagctcg gcgatgaccc ggccgcgccg cggttcatcg agaccgtccg
     1441 gcgggtcggc taccgcctgc gcgagggtgc ctgatggtgc cgcgatcgct cccgttacgg
     1501 ctgctggcgg cgttcctcgt cgtcgtcgcc gcatcgatcg gcaccgtcgg ggttgccgtc
     1561 ctcctcttcg gcccgggcta cttcgccgag gcgatggggc acatgcccgg cgacccgatg
     1621 ggcgacgcga tgggccaggc gacacaggcg gcgttcgcgg acgcgatgcg ccaggcgatc
     1681 gtcgcggcga ccgtcgtcgc gatcgtgacg gccagcgtcg tcagcctcgc ggtcgcggcc
     1741 cggattgccc gcccgatcac cgccctggcg gcggccgccc ggcgggtcgc cggtggcgac
     1801 tacgccgagc gggtcccggt cgatgagccg ggtgagctgg ccgagctcgc gacctcgttc
     1861 aacgacatgg cgggctcgct cgaggcgacc gagcgccggc gtctccagct cgtcggtgac
     1921 gtggcccacg agctgcggac gccgctgacg acgctcgacg gctatctcga gggtctcgaa
     1981 gacggcgtga tcgagccgaa ccccgagacc tggcggctcc tgcgcgcgga gaccggccgg
     2041 ctgacccgcc tcgtcgccga cctctccgag ctgtggcgag cggaggcgcg ccagctgtcg
     2101 ctccggctcg agtcggtcga cctcgtcggc gtcgcgcgcg atgtcgtcga gcggttcgtc
     2161 ccggtggcgg cggcgaagtc gatccatatc gcggtcccgc cgacgccgct cctcgccctc
     2221 gcggaccacg accgcgttgc ccaggtcatc gggaactacc tctcgaatgc gatccgccat
     2281 gcgcccgacg gctcgacggt cacgatctct gcggcgacga ccgcgggccg ggcggtcctc
     2341 ggcgtgaccg atcgcggatc cggcctcgcc gacgaccagc tcgaggctgt gttcgagagg
     2401 ttctatcggg tcgacccggc ccggagccgc tccgccggcg gatccgggat cgggctcacg
     2461 atcgcccggg cgctcgccca ggcgatgggt ggctgggcct gggccgaacg gccgcgcgac
     2521 gggatcggcg tcaccttcct gctggatctt ccgcccgcct gacccgtcga cccccggccg
     2581 gtcgagcccg caggaatcga aacgacggcg agacccgcgt gggtcccgcc gccgtcgtcg
     2641 cttcggggtc agacgttgag gcgtgcggtg cagacctcgc cggtcgtggc gttgacggcc
     2701 cgggcggcga tcgtgtcggt gccggcgcgg ttcgggatca ggaggcgaac ggtgaacgag
     2761 ccgctgggag cgacggtccg cttgctgccg cgatagacga gatgggtatc gtcggtgacc
     2821 cggacgtacc acagacggtt gctgcggttc tggtcgacct cgaactcgac ttcgatgcgg
     2881 ccgttgtcgt gcttggcctt gagggccgac gtgctgtggc cggtgcaatg accgaccgcc
     2941 cggacggcgg atccgccgct ggcgagggtg gccaccggcg cggcgatgct gccgagggcc
     3001 atggcgaggg cgaggccgcc gctggcgatg agggcggtcg ggcgaggcag ggcgaagcga
     3061 ggcatggtgc tctcctggtg tgcggtccgg gggtccggcg cttcggcatc tggtctggtt
     3121 caccggcggt gccgtggcct ccggcgatct ggtcacacca tcgcgcctcg acccccgccg
     3181 gcggaacggg ccgcggtcgc gaaatggctc agacctaggt ccgatcggac cgcgaccgtg
     3241 gtccccgtcg cgccacggac tcggcgcgag cggcgcgcgg acaccggagc tagccgattg
     3301 agatttcccg ccagcttgaa cccgcatctg tggtctcgta gatacggccg aagcgcgtga
     3361 agacccagcc catggtcggg ctcacgaagg tcgcgcgaac gagggtgtcg ggcccgaagc
     3421 cgccgacgcc atccggcggc aatggccaac ggtaaccggt ccacgttcga cccccgtcag
     3481 cggtggccgc cacgccgaag tggtccagat cgagggaacc cagcgcccac gcgcggtcag
     3541 gaccgacgat cgcctgcacc cccagaagat tgcccggatc gaagagccgg gagggttcgg
     3601 cccagactgg atcgacgagg ccggccgcac aggcagcgag tcccgccgcc gcgttgaccg
     3661 gggcccaggt cccgccaccg tcgttggtca tgtcggccgg cgtgtcaaac atgccagcac
     3721 aaccatggcg cacgtcggcg aaggacacgc cgccgttggg cgcgttcggc accgcgtgcc
     3781 acgtccggcc gccgtcggtc gtggccagca ggccgccgac tgtggctgcc cagccatgct
     3841 cggcatcgac gaaggacagg accgggtcgc gatcgggcgt cagggcgtcc gccgacagtc
     3901 gacccactgc acgccagtcg gccccgccgt cgtcggttcg aagcatcg
//
LOCUS       DATAIW010000004        12329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183, whole genome shotgun sequence.
ACCESSION   DATAIW010000004 DATAIW010000000
VERSION     DATAIW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHM48_00070"
     CDS             <1..273
                     /locus_tag="VHM48_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753823.1"
                     /translation="REGRRPTGDDDPAPHGGGRGRDLCRRHGRGRPARGYGEAITRRV
                     LRVARDGGLRIAVLQAFPAGRGVYERIGFREQGRLRLHEWPATAFP"
     gene            326..1261
                     /locus_tag="VHM48_00075"
     CDS             326..1261
                     /locus_tag="VHM48_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2753824.1"
                     /translation="MTIRFGAQLWSQQTTWSDFRDAAIAAEAAGWDSVWTWDHLLAIF
                     GPWEQPILEGWTTLAALGPITSRIRLGLMVGANTLRNPGLTAKLATTLDHVSGGRAVL
                     GIGGAWFEREHDAFGFDEAWGSGPGERLDRLDESVMVMRRLLDGERFDHTGRFYTMRD
                     ALCEPRPVQAHLPILVGGSGPKKTLRIVALRADGWNTSGTIDEARATLAILDEHCAAV
                     GRDRSAIELSVSFPITIRDTVAGAERAYGTLLQANGIEDMRTNPVLLGPPDLVAEAIA
                     PYRDLGFSTVIVRLPAPHDRETIDRMAEVSALLGG"
     gene            1254..2198
                     /gene="cofD"
                     /locus_tag="VHM48_00080"
     CDS             1254..2198
                     /gene="cofD"
                     /locus_tag="VHM48_00080"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX2753825.1"
                     /translation="MGEPRVVELAGGVGGAKLAEGLQTVVGERLTVVVNTADDTERHG
                     LLVCPDHDTVLYTLAGLENPEMGWGLAGDSWTTMGAMERYGEEGWFRLGDLDLATHIV
                     RTDRLRRGGRLSDVLLGLQRSLGLRATILPMADEPIRTQVRTGDRWLDFQDYFVRLRQ
                     APDVHEVRFTGIEAARPTAEVVAALAAADIVVIAPSNPIVSVGPILAVPGMTELLAGA
                     RARGTRFVAVSGIIGGKALKGPADRMLASLGHEPSALGVARGYAGLIDAFVLDTVDAE
                     LAPAVEALGIRTLVTDTIMTDDASRARLAAAVLAAVGA"
     gene            complement(2151..2984)
                     /locus_tag="VHM48_00085"
     CDS             complement(2151..2984)
                     /locus_tag="VHM48_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753826.1"
                     /translation="MMDEGQVPSASQEAPAGPGAASSGDTTADRPLDDPRSLQILSTE
                     HWSLLATRSMSWNESFSRTGLFLSVLSASVVALGLVGGATGFGSEFPVFALSLLPVTL
                     FVGVATFVRLDEVNLEDAIWVAAMNRIRHAYLEAKPGLEPYFTTGWTDDEYGIAQTFA
                     MTREPTAANTLVHQFVTTPGMVAVIDGALAAAISGIGLAAISGPTGMLATIPVGLIVG
                     VVTTTILMWTSVRRGRRVMRGWQSRFPRGGPPERIPMMGYWGERSGADSGKDGGGEAG
                     A"
     gene            3113..3877
                     /locus_tag="VHM48_00090"
     CDS             3113..3877
                     /locus_tag="VHM48_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2753827.1"
                     /translation="METQPQDARRALVLHDRELVVDLIELTLNHGLFVVRSARHLAEA
                     ESILAEWHPHMTVIDMDHDDSTALLHRIGASNTLRRSVTPVLGLTRRGDLKTKLRAFD
                     LGVDDILTMPFSPEELLARSIVITRRASGADHPIIPTITLGEMEIDIVNRQVRAGQSI
                     VHLSGIEQSLLYLLASRAGKVVTRDEILDAIWGTDFVAESNIVDRHIRSLRIKLQNDY
                     RHPRFIATVAGTGYRFIPTFSDTGWAGEPTTPDAPD"
     gene            4316..4576
                     /locus_tag="VHM48_00095"
     CDS             4316..4576
                     /locus_tag="VHM48_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753828.1"
                     /translation="MGASVDDASPAICDVLAAWRAAERELVDLDENDPEWSRVHAEFV
                     GLRALHHRLFEARMGPGNAGGGTAATGSASFLTLGPPVLATV"
     gene            4986..5609
                     /locus_tag="VHM48_00100"
     CDS             4986..5609
                     /locus_tag="VHM48_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX2753829.1"
                     /translation="MDPTFLVRGLAIGFTVAAAVGPISLLTMRRTIAHGRVYGLVSGL
                     GVASADASYGAVAAFGLTAITTALVGARTALALVGGAFLVWLAIRTIRARPASSAALA
                     DDRPGLPGAFISIYGLTMTNPMTILSFAGIFAGLGLGGRGGVDAALLTLGVFLGSSLW
                     WVVLTAIVARLRERITPRALLWINRVSGSVLLGFGVVAIAVALGAAR"
     gene            5670..6323
                     /gene="cofC"
                     /locus_tag="VHM48_00105"
     CDS             5670..6323
                     /gene="cofC"
                     /locus_tag="VHM48_00105"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX2753830.1"
                     /translation="MPSPARPGGGPVVAIVPVGSLDGAKSRLGEVLDAEERLDLTLRL
                     ARATIGAAVAAEQVDDVLVITPDETVRSLALELGARPILQRDNGLNHGLVAARGEAIA
                     AGAVAILILPIDLPDVTPAAIDAVAATLEEPQRPLVAIVPDRHGRGTNALLIAPPDAI
                     DLCYGGASAIAHQAAARAAGARLVVLDGPLTLDLDTPDDLLLAEASLASRAQPVDVG"
     gene            6313..7158
                     /gene="cofE"
                     /locus_tag="VHM48_00110"
     CDS             6313..7158
                     /gene="cofE"
                     /locus_tag="VHM48_00110"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2753831.1"
                     /translation="MSADVPTGADVPAGAPLPEAAGGATESSGRIEAIALAGIPEIGV
                     GDDLAGLIGDAIEATAGLLPLRPDDVLVVTQKIVSKSEGAVVDLEAIEPRPEAVAFAA
                     RWDRDPRQVEVVLREARRVVRMERGVIITETEHGFVCANGGVDASNVGPASGHIVTLL
                     PHDPDGSARAIREAVRARFGIDVPVIVSDSFGRPWRWGITDVAIGVSGLVPIEDLRGT
                     PDADGRVMKSTIRASADEIASAAELALGKVAGRPVALVRGARPLLGEGSIRDAIMPRE
                     FDLFG"
     gene            7394..8170
                     /locus_tag="VHM48_00115"
     CDS             7394..8170
                     /locus_tag="VHM48_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753832.1"
                     /translation="MTTTTTRAGPEEPAGRPSAAGSAARLTVRPPAWLDRALEVAIVA
                     TKTATIACAIDGFVNADTPRLRGKAIRTRAIGYTAALFIVPSIWRLMPERGRYPRGLD
                     LAVTLPLLIDAGGNALGLYEEAHLDDVVHFLNSAIVSGVAGSLFGTRTDEPWQAALAG
                     TGTAIAGETVWEITEFLAMKAGADGMDLTYADTMADLTESALGAVVGGIVTWLRMPRD
                     RAERRQGWRHAVAGWRRAGEPVALIAGRGPAAEPATASTG"
     gene            8167..8976
                     /locus_tag="VHM48_00120"
     CDS             8167..8976
                     /locus_tag="VHM48_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2753833.1"
                     /translation="MTATADDPAPSALPITTVGVRYLGHATVLLELPGIRILTDPFLR
                     DRLGPLRRYGPLPRPGVIGEIDVVAISHAHPDHFDRRSLQSLAGSPLVIVPRGMGGRL
                     RGLGLDVREVAVGERLAIAPDRTLTAVPARHWRWPGAPRAAAVGYLIEGLDGPGRGGI
                     YFAGDTAPYRGMRAFADRVDIALLPVGSWGPHLTPGHLTPRSAAEVARDLGARVAVPI
                     HWGTLYPPRLERFFGSRLTEPASRFVAWAGRLAPDLDVRALQPGEATTIEI"
     gene            complement(8973..10226)
                     /locus_tag="VHM48_00125"
     CDS             complement(8973..10226)
                     /locus_tag="VHM48_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2753834.1"
                     /translation="MDSSNHAAPATPATASIVRPAIVYSILFGAQGAYLPYIPIYLAS
                     TGLDLGTVGALIALFAAVSLVAAPAWGALADGIGDVRGPVIVAGFLSGGAVLLLAVAV
                     GPLALAVATALLAATWAGVIPMIDSQAVRLVGHRDRFGQARGPGSGAFVVVAFTTGVV
                     LGTAGPGGMFLVFGPLVILTGLGAWFLLRLPAPAATSARVSRPRGSGAAGRALAGISP
                     STILSVLRTPRLGVFFVASIVVWTSHAAIQGFISLRVKELGGDATMIAATWSLGALIE
                     VPLMSAFPAFARRFGAERLIVIGAFAFAIRAGISALVDAPAAIVMASAFGGIGFSFFY
                     VGTVTWVAGSVGRSVQATAQGIVSGTANSVGAIAGSIVGGAIGGVFGLPALFGAAAVG
                     YSVGAILVWLAIARGVEAAPRHRAG"
     gene            complement(10339..11361)
                     /locus_tag="VHM48_00130"
     CDS             complement(10339..11361)
                     /locus_tag="VHM48_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753835.1"
                     /translation="MRIQRSAAVAATFVLAALPALASGAVAGGGADPQAARQTAEHGR
                     IISYWTEARMRAAVPRDFVFDSVRGAQIAPQAKPGGGSGGDTTGASWPNGKGKVYKAT
                     GKVYFVMGPSAYVCSGTALSNSRGGTDGVVITAGHCAYDETAGAFATNWLFIPQFDSS
                     PTFTCANTAYGCWTAKALVVHNGFASAGGFNNSAIKYDWSFAVVGGGGKTAGANLDLD
                     TTVGKFGYLASEMALNTVADAFGYPAAQKYHGNDLTYCQGPTTRDSNAGTNNYKLACG
                     MTGGSSGGPWFSGFNTTTGDAGTIQSLNSYGYSGQSFMYGPIFNGNTTTTYNAANSAT
                     TNTIVN"
     gene            complement(11560..>12329)
                     /locus_tag="VHM48_00135"
     CDS             complement(11560..>12329)
                     /locus_tag="VHM48_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX2753836.1"
                     /translation="RDELGHEPGDTASAPDGPRGDEARERAAALETVLAIVDEVIRAD
                     PAADRDRIRQELERRAAHERDGAADGVNLLTYHRAKGLEWDAVFLPMLEDGTLPIRQA
                     FEDVAAIAEERRLLYVGITRARRQLHLSWADRRETRGREVRRTRSRFLTGLAPAKPRS
                     AGTGRGLLPSPTKVRARPAAGEDPLFDALRAWRTERAREETMPAYVVAYDETLASIAA
                     ARPTTLAALERVKGIGPAKLDKYGADILAIVAAAARD"
BASE COUNT         1735 a         4494 c         4319 g         1781 t
ORIGIN      
        1 cgggagggtc gacggccgac cggtgacgac gacccggctc cacacggcgg cgggcgtggc
       61 cgggatctat gccgtcgtca cggccgaggg cgcccggcgc gcggctacgg cgaggcgatc
      121 acccgccggg tcctgcgggt cgcccgtgac ggcggcctcc ggatcgcggt cctccaggcg
      181 tttccggccg ggcgcggagt ctacgagcgg atcgggtttc gcgagcaggg ccggctccgc
      241 ctccacgagt ggccggcgac ggcgtttccc tgaccggcgc cggccagccg gcacgccccg
      301 gggctcgatg ccggatgatg tcgacatgac gattcggttc ggtgctcagc tctggtccca
      361 gcagaccacc tggtcggact tccgcgatgc cgccatcgcg gccgaggcag ccggctggga
      421 ctcggtctgg acatgggatc atctcctggc gatcttcggg ccgtgggagc agccgatcct
      481 cgaaggctgg accaccctgg ccgccctcgg cccgatcacg agccggatcc ggctcggcct
      541 gatggtcggc gccaataccc tccgcaaccc gggcctgacc gccaagttgg cgacgaccct
      601 cgatcacgtc agcggcggcc gggcggtcct cggcatcggc ggcgcctggt tcgagcgcga
      661 gcacgacgcc ttcggcttcg acgaggcgtg gggctcaggc ccgggcgagc ggctcgaccg
      721 gctcgacgag tcggtgatgg tgatgcgccg cctgctcgac ggcgagcggt tcgatcacac
      781 gggccgcttc tacacgatgc gcgacgccct ctgcgagccc cggccggtcc aggcccacct
      841 cccgatcctc gtcggcggct ccggtccgaa gaagacgctc cggatcgtcg cgctgcgggc
      901 ggacggctgg aacacgtcgg ggacgatcga cgaggcccgc gccacgctcg ccatcctcga
      961 cgagcactgc gcggcggtcg ggcgcgatcg ttccgcgatc gagctctcgg tcagcttccc
     1021 gatcacgatc cgcgacacgg tcgcgggagc cgagcgggcc tacggcacgc tcctgcaggc
     1081 caacgggatc gaggacatgc gaacgaaccc cgttctcctc gggcccccgg accttgtcgc
     1141 cgaggcgatc gcgccctacc gcgacctcgg cttctcgacc gtcatcgttc gcctcccggc
     1201 accgcacgat cgcgagacga tcgatcggat ggccgaggtc tcggccctgc tcggtgggtg
     1261 aaccgcgcgt cgtcgagctt gccggcggcg tcggcggggc gaagctcgcc gaagggctgc
     1321 agacggtcgt cggcgagcgg ctgacggtcg tcgtcaacac cgccgacgac accgagcgcc
     1381 acggcctcct cgtctgtccc gatcacgaca ccgtgctcta caccctcgcc gggctcgaga
     1441 acccggagat gggctggggt ctcgccggcg acagctggac gacgatgggg gcgatggagc
     1501 gctacggcga ggagggctgg ttccgtctcg gcgacctcga cctcgcgacc cacatcgtgc
     1561 ggaccgatcg cctgcgccgc ggcggccgcc tgagcgacgt gctgctcggc ctccagcgct
     1621 cgctcggact ccgggcgacg atcctgccga tggccgatga acccatccgg acgcaggttc
     1681 ggaccggcga ccgctggctc gacttccagg actacttcgt ccggctccgc caggcgcccg
     1741 acgtccacga ggtccgtttc accggcatcg aggccgctcg tccgacggcc gaggtcgtcg
     1801 ccgccctggc cgccgccgac atcgtcgtca tcgccccctc gaatccgatc gtctcggtcg
     1861 ggccgatcct ggccgtcccc ggcatgaccg agctcctcgc cggcgcccgg gcccgcggga
     1921 cccgcttcgt ggccgtcagc gggatcatcg gcggcaaggc gctgaaaggc cccgcggacc
     1981 ggatgctggc gtcgctcggc cacgagccga gcgccctcgg ggtcgcccgc ggctacgccg
     2041 gcctgatcga cgccttcgtc ctcgacacgg tcgacgccga gctcgcgccg gccgtggagg
     2101 cgctcgggat ccggacgctc gtgaccgaca cgatcatgac cgacgacgcc tcacgcgccc
     2161 gcctcgccgc cgccgtcctt gccgctgtcg gcgcctgacc gctcgcccca gtagcccatc
     2221 atcgggatcc gctctggcgg cccgcccctc gggaagcgag actgccagcc gcgcatgacc
     2281 cggcggccgc ggcggaccga ggtccacatc aggatcgtcg tcgtcacgac cccgacgatc
     2341 agcccgaccg ggatcgttgc cagcatgccg gtcgggccgg agatcgcggc caacccgatg
     2401 ccggagatcg cggcggcgag cgcgccgtcg atcaccgcga ccattccggg cgtcgtgacg
     2461 aactggtgga cgagggtgtt ggcggccgtc ggttcccggg tcatggcgaa ggtctgggcg
     2521 atgccgtact cgtcgtcggt ccagccggtc gtgaagtacg gctcgagacc tggcttcgcc
     2581 tcgagatagg catgccggat ccggttcatc gcggccaccc agatcgcgtc ctcgaggttc
     2641 acttcatcga gccggacgaa ggtcgccacg ccgacgaaca gcgtcacggg caggagcgag
     2701 agggcgaaca ccgggaattc gctgccgaac ccggtcgccc cgccgacgag gccgagcgcg
     2761 acgaccgacg ccgacaggac cgacaggaag aggcccgtcc ggctgaacga ctcgttccag
     2821 ctcatcgagc gggtcgcgag caggctccaa tgctcggtcg agaggatctg gagcgaccgc
     2881 gggtcgtcga gcggtcggtc cgccgtcgtg tccccggaac tcgcggcgcc cggtccggcc
     2941 ggagcctcct ggctcgcgct cggcacctgt ccttcgtcca tcattcgcca tgccctcccg
     3001 cgtccccgat gtccgtgcct ggcgaggata cggaacccct gcgtggtccc gggaccagcg
     3061 ccgggccgtc tggcacttcc accatcttga cggggccagt aggttcacgc caatggaaac
     3121 gcaaccgcag gatgcccgcc gggcgctggt cctgcacgat cgcgagctcg ttgtcgacct
     3181 gatcgagctg acgctgaacc acgggctgtt cgtcgtccgg tcagccaggc acctcgccga
     3241 ggccgagtcg atcctcgccg agtggcaccc gcacatgacg gtcatcgaca tggatcacga
     3301 tgacagcacg gcgctcctcc atcggatcgg tgcctcgaac acgctccgcc ggagcgtgac
     3361 cccagtcctc ggcctgacca ggcggggtga tctcaagacg aagctccggg cgttcgacct
     3421 cggcgtcgac gacatcctga cgatgccgtt ctcgccggag gagctgcttg cgcggtcaat
     3481 cgtgatcacc cgtcgggcgt cgggtgccga ccatccgatc attccgacga tcaccctcgg
     3541 cgagatggag atcgacatcg tgaaccgcca ggtccgggcc gggcagtcga tcgtccacct
     3601 gagcggaatc gagcagagcc tcctgtacct gctcgccagc cgagccggca aggtcgtgac
     3661 ccgcgacgag atcctcgacg cgatctgggg caccgatttc gtcgccgaga gcaacatcgt
     3721 cgatcgtcac atccggagcc tcaggatcaa gctccagaac gactaccgtc atccgaggtt
     3781 catcgccacc gtcgcgggaa cgggctaccg cttcattccg accttctcgg acaccggctg
     3841 ggcgggcgaa cccacgacgc cggacgcacc ggactgagcc gctccgccgt ctggtggacg
     3901 acatgacaag gacgtcatga cgggtcgccg cggacggtcc atcgtggcgc tggatcgata
     3961 tccgggcctg tgacgacggg tcgaacggat acgtcgcgag cggcccgtcg gcggggacgg
     4021 gttcgtttct gtcggagcga gtgacggcga tcgaccggga tcgcggcggc gaagggcgtg
     4081 caagatgaca gctggggtcg aggcaccggt cgcaggtcgg ggaccgaggg tcgcgagcga
     4141 gtcggaggcc ctcttcggtc gcccacgctc ccggatcgac caatggattt cgctcgacgc
     4201 cgacacctgg caccacgacc gatccagccg ccgccccgag cccgtgaaat cgcgaccgtc
     4261 gccgtacgag gcagtcccga catcgagtct cacggcggcg cggccgcgga ccgcgttggg
     4321 cgcgagcgtc gacgacgcct cgcccgcgat ctgcgacgtg cttgcggcct ggcgagcggc
     4381 agaacgtgag cttgtcgacc tggacgagaa cgatcccgag tggagccgtg tgcatgccga
     4441 attcgtcggc ctgcgcgcgc tccaccaccg cctgttcgag gcgcggatgg gcccaggaaa
     4501 cgccggcggc ggcacggccg ccaccgggtc cgccagcttc ctgacgttgg gcccgcccgt
     4561 gctcgcgacc gtctgatcgc gtgaaccgac ttcgcgcgat cgtcggctcg gacggccttc
     4621 gcagcgccgc ccttgcgatc gccgcggtcc tgctgatcct cgtcgccctg ccggtcgtgc
     4681 tcatggcggc cggaacctga aggcccgggc cggacgtcgc gcgtcgcgtc tcccgggcgg
     4741 tccgggacgt tgacaggcgg accccgttct tgcacagtgc gcgcgatgca ccacatcgcc
     4801 gccgccgtca tctgtcgtgc gacgctcggc cagcagcagc cctcgacggg ggtcggcctg
     4861 gcctgacgac ggcacccggt cgggtgcatc gatcaccacc gccctcggct cggccggggg
     4921 ccttttcgtg tcgcgaacgc ccatcccccg atccgcgccg gcggcaccag ccacggagca
     4981 gcacgatgga tcccacgttc ctcgtccgag gtctcgccat cggcttcacg gtcgcggccg
     5041 cggtcggccc gatcagcctc ctgacgatgc gccgaacgat cgcccacggt cgggtgtatg
     5101 gcctcgtctc cggcctcggc gtcgcatcgg ccgacgcgtc gtacggcgcc gtggccgcct
     5161 tcggcctgac ggcgatcacg acggccctcg tcggggcgcg gacggcgctc gccctcgtcg
     5221 gtggtgcgtt cctcgtctgg ctggcgatcc ggaccatccg ggcccggccg gcgtcgagcg
     5281 ccgcgctcgc cgacgatcgg ccgggtcttc cgggcgcctt catctcgatc tacgggctga
     5341 cgatgaccaa cccgatgacg atcctctcgt tcgcgggcat cttcgcgggg ctcgggctcg
     5401 gcgggcgcgg cggggtcgac gccgcgctgc ttacgctcgg ggtgttcctc ggctcgagcc
     5461 tgtggtgggt cgtcctgacg gcgatcgtcg cgcgccttcg tgagcgaatc accccgcgcg
     5521 ccctgctctg gatcaaccgc gtctcgggga gcgtgctcct cggcttcggc gtcgtggcga
     5581 tcgcggtcgc cctcggcgct gcacgctgag ccgaccgttc tcgcggagga gttcggaccg
     5641 acggcccgat ctcgcctagg atttcgatca tgcccagccc agctcgtccc ggaggaggac
     5701 ccgtcgtcgc gatcgtgccc gtgggctcgc tcgacggggc gaagtcgcgg cttggcgagg
     5761 tcctcgatgc cgaggagcga ctcgacctca cgctcaggct tgcccgcgca accatcgggg
     5821 cggccgtggc ggccgagcag gtcgacgacg tcctggtcat cacccccgac gagacggtcc
     5881 gctcgctcgc actggagctc ggggcgcgac ccatcctcca gcgtgacaac ggcctcaacc
     5941 acggtctcgt cgccgcacgt ggcgaagcga tcgccgcagg cgcggtcgcg atcctcatcc
     6001 tgccgatcga cctgccggac gtgacgccgg cggccatcga cgccgtcgcg gcgacgttgg
     6061 aggagccgca gcgacccctc gtggcaatcg tccccgatcg ccacggccgc ggcacgaacg
     6121 cgttgctgat cgctccgccc gatgcgatcg acctgtgcta cggcggcgcc agcgcgatcg
     6181 cccaccaggc ggctgcccgg gcggccggcg cccgtctcgt cgtcctcgac gggccgctca
     6241 ccctcgatct cgacacgccg gacgatctgc ttctcgcgga ggcgagcctg gccagccgag
     6301 ctcagcccgt cgatgtcggc tgatgtcccg accggggcag atgtcccggc cggagccccg
     6361 ctcccggagg ccgccggcgg cgcgacggaa tcgtcgggtc ggatcgaggc gatcgccctc
     6421 gccgggatcc ccgagatcgg cgtcggcgac gatctcgccg gcctgatcgg cgacgcgatc
     6481 gaggcgaccg ccggcctcct gccccttcgg cccgacgacg tcctcgtcgt gacccagaag
     6541 atcgtctcca agtccgaggg cgccgtcgtg gatctcgagg cgatcgaacc acgtccggag
     6601 gcggttgcct tcgccgcgcg ctgggaccgc gatccgcgcc aggtcgaggt cgtcctgcgc
     6661 gaggcccggc gggtcgtccg gatggagcgc ggcgtgatca tcaccgaaac ggaacacggc
     6721 ttcgtctgcg ccaacggcgg ggtcgacgcg tcgaacgtcg ggccggcgag cggccacatc
     6781 gtgaccctcc tgccgcacga ccccgacggt tcggcacggg cgatccgcga ggccgtccgc
     6841 gcccggttcg gcatcgacgt cccggtgatc gtgtccgact cgttcgggcg gccgtggcgc
     6901 tgggggatca ccgacgtcgc gatcggcgtg tccggcctcg tgccgatcga agacctccgc
     6961 ggcacgcccg acgcggacgg ccgggtcatg aagtcgacga tccgggccag cgccgacgag
     7021 atcgcgtcgg ccgccgagct ggcgctgggg aaggtcgccg gccgcccggt cgccctcgtc
     7081 cgcggcgccc ggccgctcct cggcgaaggc tcgatccggg acgcaatcat gccccgcgag
     7141 ttcgacctct tcggctgatc cggcgaccgc ggatctcgcg ccgccgtttc cgacgcgacc
     7201 gtggagcagc catcgaggcg cggcgagcgc ggatcgtcgc gagcagggcg gcggaagtta
     7261 tccacagcga ttgtggattg tcaggcagat gcaatgtcgg ccgcagcctc ccgacgcccc
     7321 acattcgcga ttgcaatgcc gattcggtcc ggacgagtcg tcgcgatgac gcaggatcgg
     7381 gtacacctcg ctcgtgacca cgacgaccac ccgggcaggt cccgaggagc ccgccggccg
     7441 accatcggcc gccggctccg cggcgcgcct caccgttcga ccgccggcct ggctcgaccg
     7501 cgccctcgag gtggcgatcg tagcgacgaa gacggccacg atcgcctgtg cgatcgacgg
     7561 cttcgtcaac gccgacacgc cacgcctacg cggcaaggcg atccggacgc gggccatcgg
     7621 ctacacggcc gcgctgttca tcgtgccttc gatctggcgc ctcatgccgg aacgcggccg
     7681 ctacccgcgc ggcctcgacc tcgccgtcac cctgccgttg ctgatcgatg ccggtgggaa
     7741 cgcgctcggc ctgtacgaag aggcccatct tgatgacgtc gtccatttcc tgaactcggc
     7801 gatcgtcagt ggcgtggccg ggtcgctctt cggaacgagg accgatgagc cgtggcaggc
     7861 ggcgttggcc gggaccggca cggcgatcgc gggcgagacc gtctgggaga tcaccgagtt
     7921 cctcgcgatg aaggccggcg cggacggcat ggacctgacc tacgcggata cgatggccga
     7981 cctcaccgaa tcggcgctcg gcgccgtcgt cggcgggatc gtgacctggc tgcggatgcc
     8041 gcgcgatcgg gccgaacggc ggcagggctg gcggcatgca gtcgcgggct ggcggcgcgc
     8101 cggcgagccg gtggcactca tcgcggggcg gggcccggcg gccgagcccg cgacggcatc
     8161 cactggatga ccgcgaccgc cgacgatccc gctccatcgg cgctgccgat caccacggtc
     8221 ggggtgcgct atctcggcca tgccacggtc ctgctcgagt tgccggggat ccggatcctg
     8281 accgatccgt tccttcggga ccgtctcgga cccctccgcc ggtatggccc gctcccccgg
     8341 cccggcgtga tcggtgagat cgacgtcgtc gccatctccc acgcgcaccc cgatcatttc
     8401 gaccggcgat cgctccagtc cctggccggc agcccgctcg tgatcgttcc gcgcgggatg
     8461 ggtggacgcc tccgcgggct cggactggac gttcgcgaag tcgcggtcgg cgagcgcctg
     8521 gcgatcgctc cggaccggac gctcaccgcg gtccccgccc gtcactggcg ctggcccggc
     8581 gccccgcggg cggccgcggt gggctacctc atcgaggggc tcgatgggcc cggccggggc
     8641 gggatctact tcgccggcga caccgcaccg taccgcggca tgcgcgcgtt cgccgaccgg
     8701 gtcgacatcg ccctcctccc ggtcggaagc tggggtccgc acctgacgcc cggccacctg
     8761 acgccccgat cggcggccga ggtggcccgt gacctcggcg cccgggtggc ggtcccgatc
     8821 cattggggga cgctctatcc gccccgcctc gagcggttct tcggcagccg cctgacagag
     8881 ccagcatccc gcttcgtcgc gtgggccggc cggctggcgc cggacctcga cgtgcgtgcg
     8941 ctgcagcccg gcgaggcgac cacgatcgag atctagccgg ctctgtgccg cggcgcggcc
     9001 tcgacgccgc gagcgatcgc cagccagacg aggatggcac ccaccgaata cccgaccgcg
     9061 gcggcgccga agagggccgg cagaccgaat acgccgccga tcgcgccgcc cacgatcgag
     9121 cccgcgattg cgccgacgct gttggcggtc ccgctgacga tgccctgggc cgtggcctgg
     9181 acgctccgcc cgaccgagcc tgcgacccac gtcacggttc cgacgtagaa gaacgagaac
     9241 ccgatgcccc cgaatgcgga cgccatcacg atcgccgccg gggcatcgac gagcgcgctg
     9301 atccccgccc ggatcgcgaa ggcaaaggcg ccgatgacga tcagccgctc ggccccgaag
     9361 cgccgcgcga aggccgggaa ggcggacatc agcggaacct cgatcagcgc gccgagcgac
     9421 caggtggcag cgatcatcgt cgcgtcgccg ccgagctcct tgacccgcag cgagatgaag
     9481 ccctggatcg cggcgtgcga ggtccagacg acgatcgagg cgacgaagaa cactcccagg
     9541 cgcggggtcc gcagcacgct gaggatcgtc gagggcgaga tgccggcgag cgcccgaccg
     9601 gcagcgccgg agccgcgggg ccggctgacc cgggccgatg tcgcggctgg cgcgggtagc
     9661 cgaagcagga accacgcccc gagcccggtc aggatcacca gcggcccgaa gacgaggaac
     9721 attccgcccg gcccggccgt tccgaggacc acaccggtcg tgaacgcgac gacgacgaac
     9781 gcccccgatc cgggtccgcg cgcctgaccg aagcgatcgc gatggccgac gagccggacg
     9841 gcctggctgt cgatcatcgg gatgaccccg gcccaggttg ccgcaagcaa cgccgtcgcg
     9901 accgcgaggg cgagcggccc gaccgcgacc gccagcagca acacggcccc gccggacagg
     9961 aatccggcca cgatgaccgg cccccggaca tcgccgatcc cgtccgccag cgcgccccag
    10021 gccggcgcgg cgacgaggct gaccgcggcg aacaaggcga tcagggcgcc gacggtcccg
    10081 agatcgaggc cggtcgaggc aaggtagatc gggatgtatg ggaggtaggc accctgggcg
    10141 ccgaacagga tcgagtaaac gatcgccggc cggacgatcg acgcggtcgc cggcgtcgcc
    10201 ggggcggcat ggttcgagga atccatcgtc ggtagcgtgc ccgatcgacg cgactcccgt
    10261 cacggaacga ccccgggcga accgtcagac ggtccgcccg cgggttcgtt gtccgccgac
    10321 ctcagtcggc agcggcaatc agttgacgat cgtgttggtc gtcgcgctgt tcgccgcgtt
    10381 ataggtcgtg gtcgtgttgc cgttgaagat cgggccgtac atgaacgact gcccggagta
    10441 gccgtacgaa ttgagcgact ggatcgtgcc cgcgtcgccg gtcgtggtgt tgaagcccga
    10501 gaaccacggg ccgccggacg agccgccggt catgccgcac gccagcttgt agttgttcgt
    10561 gccggcgttc gagtcgcgcg tcgtcgggcc ctggcagtac gtcaggtcgt tgccgtggta
    10621 cttctgagcg gccggatagc cgaacgcatc ggcgaccgtg ttcagcgcca tctcggacgc
    10681 gagatagccg aacttgccga cggtcgtgtc gaggtcgagg ttggcaccgg cggtcttgcc
    10741 gccgccgccg acgacagcga acgaccagtc gtacttgatc gcgctgttat tgaacccgcc
    10801 cgccgaggcg aatccgttgt ggacgacgag tgccttggcg gtccagcaac cgtacgcggt
    10861 gttggcacac gtgaatgtcg ggctgctgtc gaactgcggg atgaacagcc agttcgtcgc
    10921 gaacgcgccg gcggtctcgt cgtaggcgca atgcccggcg gtgatgacca ccccgtccgt
    10981 gccaccgcgg ctgttgctga gcgcggtgcc gctgcacacg tatgcgctcg ggcccatcac
    11041 gaagtagacc ttcccggtcg ccttgtagac cttgcccttg ccgttcggcc acgaggcgcc
    11101 ggtcgtatcg cctccactcc cgccgcccgg cttggcctgc ggggcgatct gggcgccgcg
    11161 aacgctgtcg aagacgaagt cgcgcgggac ggccgcccgc atccgggcct ccgtccagta
    11221 gctgatgatc cggccgtgct cggccgtctg gcgagctgcc tggggatcgg ccccgccgcc
    11281 cgcgactgcg ccgctggcga gggccggcag agcggcgagc acgaaggttg ccgcgaccgc
    11341 ggctgaacgc tggatccgca tatccactcc cgttgcgtta cgacctgggc tgggcggtag
    11401 caaacaacgg gagtgcaggc ccggtcaagc cgctgcgacg gtcggcggcc gatgcccggc
    11461 acctggcggt gcacgggggc accgatccgt cacgcgcctg caatggtcgc ggacgaggcg
    11521 acgggctcgg cgagccccgg gcccagacat caacggagat cagtctcggg ccgccgcggc
    11581 gacgatcgcg aggatgtcgg cgccgtactt gtcgagcttg gccggcccga tccccttgac
    11641 ccgctcgagg gccgcgaggg tggtcggtcg ggctgccgcg atcgaggcca gcgtctcgtc
    11701 gtaggccacg acgtaggccg gcatcgtctc ttcgcgagcc cgctccgtcc gccacgcgcg
    11761 gagggcgtcg aacaatggat cctcaccggc tgccggtcga gcgcggacct tcgtcgggga
    11821 cggcagcaat ccccgccccg tgcccgccga gcgcggtttc gccggagcca gaccggtcag
    11881 gaaccggctc cgcgtccggc ggacttcgcg gccgcgggtc tcgcgccggt cggcccagga
    11941 taggtggagc tggcggcggg cccgggtgat cccgacgtac agcaaccgcc gctcctccgc
    12001 gatcgcggcc acatcctcga aggcctgccg gatcggaagc gtcccgtcct cgagcatcgg
    12061 caggaagacc gcatcccact cgaggccctt ggcccggtga taggtcagca ggttcacgcc
    12121 gtcggcggcg ccatcacgct catgggcagc gcgtcgttcc agctcctgtc ggatcctgtc
    12181 gcggtcggcg gccggatcgg cgcggatgac ctcgtcgacg atcgcgagga ccgtctcgag
    12241 cgcggccgct cgctcgcggg cttcgtcgcc gcgggggccg tccggggcgg atgccgtgtc
    12301 gccgggctca tgcccgagct catcacgga
//
LOCUS       DATAIW010000005        13794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_268175, whole genome shotgun sequence.
ACCESSION   DATAIW010000005 DATAIW010000000
VERSION     DATAIW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268175"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..199)
                     /locus_tag="VHM48_00140"
     CDS             complement(8..199)
                     /locus_tag="VHM48_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753837.1"
                     /translation="MTPLVSALHVGLIALALASVGEARRPDAPTSQETPAIDRSTAAS
                     GAVEGRVAMPVGAGALAPA"
     gene            complement(230..604)
                     /locus_tag="VHM48_00145"
     CDS             complement(230..604)
                     /locus_tag="VHM48_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2753838.1"
                     /translation="MDDTIRAALSRGGVIDITTTGRKTGEPRRIEIVFHRIDGRMWIS
                     GMPSPRRRGWMANLADDPNLTIHLKGPVAVADLPATARIVDDPTERRHVLERVARAWK
                     RTDVDVMVATSPLIEVTIDEAA"
     gene            complement(681..1256)
                     /locus_tag="VHM48_00150"
     CDS             complement(681..1256)
                     /locus_tag="VHM48_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEX2753839.1"
                     /translation="MTTMTTDRLPFTGDDAADRLLVSDPLALLIGFALDQQVPLQKAF
                     SGPLELSRRIGGLDAKRIATMDPDAFDAAFRTSPALHRFPGNMAKRVRELAGYLVERY
                     DGDASRIWREAKDGADLHARLLALPGFGPMKAETLVAILGKRLGLAPAGWEAFVPDHM
                     TLGDVDGPEALRRYQDWKRGMKAEKRAAERA"
     gene            complement(1961..2815)
                     /locus_tag="VHM48_00155"
     CDS             complement(1961..2815)
                     /locus_tag="VHM48_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753840.1"
                     /translation="MPVSQAGAQRAPGRRSFRSLRLIAIAALASVLAVGGLAIHPEAV
                     AASGKKVVIVVGPAGSNTSDYIYNAKKIAAQARSYGATVYEIYAPHATWARVKSLSQG
                     ANVFIYLGHGNGYPSPYGSFNKYSKDGLGLDTYDGSWSHTYYGEYYVDHYLNFAPDAV
                     VILNRLCYASGNSEWGSANPTKATAIKRVDNYGAAFLRTGARAVFAEGISSTSYILYG
                     LFKTSRTMKQIFWTDPKRSGANDFTFSSSRTPGRSAIMDPSSPGRYYRSIIGDLGMTA
                     ATWRSAGG"
     gene            complement(3215..3724)
                     /gene="ybaK"
                     /locus_tag="VHM48_00160"
     CDS             complement(3215..3724)
                     /gene="ybaK"
                     /locus_tag="VHM48_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX2753841.1"
                     /translation="MAAGGTRALAELVRAGVVHTVHEYAIHEPTGPRSRSGRRPAWGD
                     DAAEALGVDPGRIHKTLIAAVDGGLAVAVVPVAAELDLKALARALGAGKAAMADPAEA
                     ERATGYVRGGISPLGQRRRLPTVVDAAALELPTIVVSAGRRGLQVELSPGDLVRLSGA
                     VVAPIARRD"
     gene            3875..5140
                     /locus_tag="VHM48_00165"
     CDS             3875..5140
                     /locus_tag="VHM48_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2753842.1"
                     /translation="MTFDTLGLSADLLRTVAEEGYTEPTPVQERAIPLVLQGRDILAA
                     AQTGTGKTAAFVLPILDLLRQHANTSFSPARHPVRVLILVPTRELAVQVDESVHTYGR
                     TVPLRSTVVYGGMPMEPQIKALRGGVEILVATPGRLLDLVGQKVANLGQVEILVLDEA
                     DRMLDMGFLPDIQRIIALLPAKRQNLMFSATFADEIRRLSKTILNDPVEVEVAPRNAT
                     VDAIHQLVYPVDRDRKEELLAHLIRKDDLRQLLVFTRTKIGATRLATWLDRQGLNAVA
                     IHSDRSQPERTRALEEFKSGEIRVLVATDVAARGLDIEDLPYVVNFELPWNPQDYVHR
                     IGRTGRAGASGEAISLVCIDETDLLRGVQRLLRKAIPWTVENGFVPDRNIEPRPLRGG
                     DMGGSRGGARPTHHPHRKPVQYRAGAGRS"
     gene            complement(5455..6078)
                     /locus_tag="VHM48_00170"
     CDS             complement(5455..6078)
                     /locus_tag="VHM48_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753843.1"
                     /translation="MPIRVEIYGAAGVAVGVVARAGRLREILESGLDLLVERAAWHPL
                     DGSTPRPSDALAIAEDDILVAVADQIEDGPVHAQWHDIGLDVGPYRVTGQMPTMPGFD
                     PGRALARPTGVFVLLRDVRIGLLGDADGPAVAQGAAFVNRYVVDRVAADLMLGFFFPG
                     AEMIVTGGHERSAAGASTAGASTAAASTVDTAPGASGPFAPTSTAAT"
     gene            complement(6238..7650)
                     /locus_tag="VHM48_00175"
     CDS             complement(6238..7650)
                     /locus_tag="VHM48_00175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2753844.1"
                     /translation="MAGALLAAAAGIAVLGIGFIRTAGGLAGAPLDGVARGSAITSGP
                     CAGAPAVASAACASPTTPGGASPSTTGAPLAPSAPAGSPSPGPGSSPGPSIDDPSAIL
                     AARLQAALDRVRARLAIPGVSATILLPDGTSWSGVSGFADVAARTRVTPDTAFAFASV
                     SKTFTSALVLELIGEGRMHLTDSAAHLLPPLRITIDRRITVGMLLNHTSGLADYFLNP
                     KIDGPLQGHPTAAWTTERTLRYVGKPLSLPGAAWHYANTNYLLLGLIAERATGQTLAD
                     AIRTRLLEPVGLAATWYQAGEAARSPLAHGYRFVGTKRTARPIDLEDGSGIAPFRSVI
                     TAAAGAGSMAGTSTDLARWARALYGGKVLGPQGTATLLSGFSKTTNYLPGVVYGYGVQ
                     AFSIDGHPSLGHSGRLLGFRSAVRHFPIDGLTIAVLTNQSRADPGVIVRALLAAALPV
                     AAPVPVPGRPPVCPTCSMAR"
     gene            7799..7930
                     /locus_tag="VHM48_00180"
     CDS             7799..7930
                     /locus_tag="VHM48_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753845.1"
                     /translation="MVRSLGFLGVRTKAFAEMAALDRDVLELEPDDFEIVGPDRSAA"
     gene            7927..9219
                     /gene="greA"
                     /locus_tag="VHM48_00185"
     CDS             7927..9219
                     /gene="greA"
                     /locus_tag="VHM48_00185"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2753846.1"
                     /translation="MSDERGATTIAADNGAAALFRAVGLMADGPVVWGRPVPARGSGV
                     FVIELPTTLHRAPIEHSRIGKWLEHVPELLLDGAHPTTRAMAARLEAFWLPSTRVLFV
                     GTTDGSIGGRIEAIRNTVLGDRRPHPGGHWLHTLTGLEGARVWWAATSAVEESEDALL
                     TAFAATIPVAEQAALHDPTVVLPFANLRTATGERKKTGLANSLVAAEPVRPTPAGRIV
                     VVAPGAADGASGLPPTRAAGGTVRRSPRPAGSTPARPRSTPATPRSTAAKRKPGDRPA
                     AETTWVSAEGMERLKAEHAELTGTKRHEVIGRIKAAKELGDLKENADYASAREEQSFL
                     EGRIKSIEATLRVATVIETPGDAVDRVTLGSTVTVEDDTGAQMTFVVVGSSEADPAAG
                     RISNVSPVGRALIGRSAGDDVAVAAPRGEVHYRIVELA"
     gene            complement(9259..9813)
                     /locus_tag="VHM48_00190"
     CDS             complement(9259..9813)
                     /locus_tag="VHM48_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753847.1"
                     /translation="MTGERPPDNRGGMILGIVLIALGGLFLADRVVGFDLADAGWPLF
                     VIIPGVLVFAWGLTMTGREGVSLAVAGGITTVVGLILAVQNATGLWATWAYAWALVGP
                     GGTGVGLIGYGLTHRDPGLVTNGLRSLGSGLALFVAFGLFFEGVIGLSGTPFLSTDIG
                     PVILIGAGVAVLLAGLFRGRRLPA"
     gene            complement(9839..10510)
                     /locus_tag="VHM48_00195"
     CDS             complement(9839..10510)
                     /locus_tag="VHM48_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2753848.1"
                     /translation="MEENARIRVLIVDDHSVVRMGLRVFFDLQDDIEVVGEAADGSEG
                     VAMARRLEPDVVLMDLLMPNMDGVTAIGRIKAERPETEIVTMTSFIEEDKVTAALEAG
                     ASGYVLKDAEAEEVASAVRAAFAGEVHLDPAVARLLAQRMRARKSGEGEPVEPLTDRE
                     KDVIRLLGRGMSNKEIGSALFITERTARTYVSNILGKLGLASRTQAALWAVEHKLAEP
                     ARDLR"
     gene            complement(10601..10987)
                     /locus_tag="VHM48_00200"
     CDS             complement(10601..10987)
                     /locus_tag="VHM48_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2753849.1"
                     /translation="MTDPAAAPAPLRVLLVDPDDRVRESLAGLLCIGQRCLVIGTAGT
                     ADGAVRLAAETAPDVVVVDPRLPGIDGTALIARLRAVAKDTRVLVLNWSETADLTSGA
                     DAYARKTFRPHELIDAVLATTRRTLA"
     gene            complement(11052..>13794)
                     /locus_tag="VHM48_00205"
     CDS             complement(11052..>13794)
                     /locus_tag="VHM48_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2753850.1"
                     /translation="LDPDRIDRWAGSRVGEDIVRRALAGRTALFTVDYLDDERFAVDG
                     ESAEVARGLGLRAIAIAPLVSERGALGTIAVFSPEPGRFGEDQANLLGALADQAAIAM
                     INASLIDELERSQTELEARAETERSLRDISARITSLGDPGEILSRVVDESRRLLRSDG
                     AHLTRMSEDGTFLVPVIVAGGMDVDTESWLKNMQFPLGGGINGLAAEEVRPIWTADYE
                     ADPRIPHEPGDDEAAVRLGLRAMAAAPLRAPAGEVIGTLAISYAKPREILPDELDLLQ
                     GLADQAAIALTNANLYELLGESEARYRYLVQNSPDLVWSIDADARFTFISDTCQRLTG
                     WRAEELLGRHFGALVHESSRAVAEIDWTAGIAADAGDGEGGRELRGRVNLLRRDGSPV
                     PAEFIAFATRDENGVFAGANGSVRDMSERDRLERELRESEERYRFLVDNSPDIIFSTD
                     AEGVFTYLSESIEKVTGFSPSELVGGHFSAVVDPLTQPAAVERWDAIVAEPSQRQVVK
                     LELIHKNGGTVPVEVSSIGATVNGVFAGIHGATRDIAERERLERELRQSEERYRFLVE
                     NSPDIIFTTDAEGRYTYYSETIERLTGFASTELLGKHFSTIIDLDTFPDAAAAWQAFI
                     DEPSRMQVQRFRMRRVDGGSVAVEVNAIGMTDAASRFAGIHGAARDISVRERLERDLR
                     RQAGELASSQERAHLARELHDSVTQALFSMTLVTRSVELLIDRDTAVAKEKLTSLRDL
                     QREALAEMRALIFELRPGNIESDGLLPALRTHTAALQGRIGLPVVVTSELTERLPLEV
                     EEVLYRIAQEALHNVVKHAAARQVELAVDRRGADVVLRIRDDGKGFDAATVPDGHLGL
                     AGMRARADKIGASYHVISRPGEGTTIEVIVPPEALDRAAGAAERPGPHDRVPPQLAE"
BASE COUNT         2012 a         4798 c         5036 g         1948 t
ORIGIN      
        1 ccgggcgtca ggcgggggcc agggccccgg ctccgaccgg catcgcgacg cggccctcga
       61 ccgccccgga cgcggcggtc gaccggtcga tcgcgggcgt ctcttgcgac gtcggagcgt
      121 ccgggcggcg cgcctcgccg accgatgcca gcgcgagcgc gatcaagccg acatggagcg
      181 cggagaccag cggagtcaac gcggcatcga acgacccgaa gtcacccgat caggcggcct
      241 cgtcgatcgt gacctcgatc agcggactcg tggcgaccat cacgtcgaca tcggtccgct
      301 tccaggcccg ggcgacgcgc tcgaggacgt ggcggcgctc cgtcgggtcg tcgacgatcc
      361 gcgcggtcgc cgggaggtcc gcgaccgcga ccggaccctt gaggtggatc gtcaggttcg
      421 ggtcgtcggc gaggttcgcc atccagccgc ggcgccgcgg ggagggcatg ccggagatcc
      481 acatccgacc gtcgatccgg tggaacacga tctcgatccg gcgcggctcg cccgtcttcc
      541 ggccggtggt cgtgatgtcg atgacgccgc cgcgtgagag cgcggcgcga atggtgtcgt
      601 ccatggaagc ctcgtgcgat cgttgcagtg gcaacgatat cgcagacggc cggaaggcgc
      661 ccggccaggc cccggacgga tcaggcccgc tcggccgccc gcttctccgc cttcatcccg
      721 cgtttccagt cctggtagcg gcggagcgcc tcgggtccgt cgacgtcgcc gagcgtcatg
      781 tgatccggca cgaacgcctc ccagccggcg ggcgccaggc cgagccgctt gccgaggatc
      841 gcgaccaggg tctcggcctt catcgggccg aagcccggca gggcgaggag ccgggcgtgg
      901 aggtcggcgc cgtccttcgc ctcgcgccag atccgcgacg catcgccgtc gtaccgttcg
      961 acgaggtagc cggccagctc gcggaccctc ttggccatgt tgcccgggaa gcgatgcagc
     1021 gccggcgacg tccggaacgc cgcgtcgaac gcgtccggat ccatcgtggc gatccgcttc
     1081 gcatcgagac cgccgatccg gcgactgagc tcgagggggc cggagaacgc cttctggaga
     1141 gggacctgtt gatcgagggc gaacccgatc aggagcgcca gcgggtcgct gacgagcagc
     1201 cggtcggcag cgtcgtcacc ggtgaacggg agtcgatcgg tcgtcatcgt cgtcatgtcg
     1261 cggatggtag gccgccgcgc gtcccccgac cagcgccgat cggacgcggt acgggcgggc
     1321 catccggtcg cctgatcgcg cgtcgcggtt cgacggcggc cgaatgccaa tcgccggagg
     1381 gtcgtcatcc gtccagcgcc accacggcca gatgtgcggg cgacttgtcg acacgcggcg
     1441 gcaggccggg cgggagcgtg accctatcgt tcggtcatcg agcgatcgat cgatcgccgg
     1501 atgaagcccg aaaggagtcg tccccgatga ccgcccagac gcagtacctc gagaccgaca
     1561 tgctcggtgc cttcccgacg ctcctgctgg tcctcgtcct cgtcctgacc ctgctcgggg
     1621 tggtcgccca cgtggcgttc gcctagatcg gcgactgctc gatcccccgg gacagaccgg
     1681 ccgcgccagt ccgagcgagc tcacgcgaga cccatggccc tggcgggttc acgatgcggt
     1741 gaatctgacc ggggcctccg cttctccggc gactacgtcg gatgacccgc ccggtggtcg
     1801 acaacgctgc tcgtggccat ccgccgatca gcaatcgcga tcgaacgatc gggccggatc
     1861 cgccgcccgc tgcccgccgc aaacgccacg accgcgcccg cctgagcgag cgcggtcggt
     1921 cggtcgcgcg acggcgtgtt cgatcgtccg ggtccgaggg tcagcccccg gcggaccgcc
     1981 acgtcgccgc cgtcatgccc aggtcgccga tgatcgagcg atagtagcga ccgggtgagg
     2041 acggatccat gatcgcgctc cggccggggg tccggctgga cgagaaggtg aagtcgttgg
     2101 caccgctccg cttggggtcg gtccagaaga tctgcttcat cgtccggctg gtcttgaaca
     2161 ggccgtacag gatgtaggac gtactgctga tgccctcggc gaagaccgcc cgagcgccgg
     2221 tccgcaggaa cgccgcgccg tagttgtcga cccgcttgat cgcggtcgcc ttggtcggat
     2281 tggccgaacc ccactccgag ttacccgagg cgtaacagag gcggttcagg atcaccaccg
     2341 cgtcaggcgc gaagttgagg tagtggtcga cgtagtactc gccgtaatac gtgtggctcc
     2401 agctcccgtc gtaggtgtcg aggcccaggc cgtccttgga gtacttgttg aaggagccgt
     2461 acgggctcgg atagccgttg ccgtggccca ggtagatgaa gacgttggca ccctgggaga
     2521 ggctcttcac ccgcgcccac gtcgcgtggg gagcgtagat ctcgtagacg gtggccccgt
     2581 acgagcgggc ctgtgccgcg atcttcttgg cgttgtagat gtagtccgac gtgttcgagc
     2641 cggcgggacc gacgacgatg acgaccttct tgccggacgc ggcgacggcc tcgggatgga
     2701 tggccagtcc gccgacggcg aggacggatg caagggcggc gatggcgatc agccgcaggg
     2761 accggaacga tcgacgacct ggcgcccgct gggcgcctgc ttgggacacg ggcaacgctc
     2821 ttgcctcctc tccgccggaa caccgtgcat ggggcgcggt gacggtgggt gatgccgccg
     2881 acgacaggag agcacgcgga cgcgcgaacg agacgcgcgg agcgcaggga tgggggcgat
     2941 cgggtgcggt ccgaccgggt ggcggggccg acgatccggg atcagcgagg gatccggacc
     3001 gggggccggg aaacgacagg gtcctgccgt ggggggcggc ggtcttctgt ttttcagcct
     3061 gtctgtcagg ggtggaatgg cctgggggcc tcgcagaagt atgccagccc ttccgcgacc
     3121 gcgcctcccc cccagattca ccatgctgtc cactgccgat gggactgggt ccgtgcacga
     3181 ggtgttgacg ggatgccttc ggccgacgat tcgatcagtc gcggcgggcg atcggggcga
     3241 ccacggcgcc ggaaaggcga acgagatccc ctggtgagag ttcgacctgg aggccgcggc
     3301 ggccggccga gacaacgatc gtcggcagtt cgagggccgc cgcgtcgacg accgtcggca
     3361 ggcgccgacg ctggccgagg ggactgatcc cgccgcggac gtatcccgtc gcccgctcgg
     3421 cctcggcagg atcggccatc gccgccttcc ctgcgccgag cgcgcgggcg agcgccttga
     3481 ggtccagctc ggccgcgacc gggaccacgg cgaccgcgag gcctccgtcg actgcggcga
     3541 tcagggtctt gtggatccgg ccgggatcga ccccgagcgc ctctgcggcg tcatcgcccc
     3601 acgccggacg gcggccggat cggctccgcg ggccggtcgg ctcgtgaatc gcgtactcgt
     3661 ggaccgtgtg gacgacccca gcccggacga gctcggcgag ggcgcgggtg ccgcctgctg
     3721 ccatcgattc atgctatcgg agcagtcatc ggctatgctc gggacgatcc gggctcgcac
     3781 cgcgtcgttt ggacggcgtc aagggaggcg agcaggagac gaccagcttg ccccgctcac
     3841 gcttccccgc gcgacgcggc gcatcaggag tctgatgacc ttcgataccc tcggcctgtc
     3901 ggccgacctg ctgcggaccg tggcagaaga gggctatacc gagcccaccc cggtccagga
     3961 acgggccatt cccctggtcc tccagggcag ggacatcctc gccgccgccc agaccggcac
     4021 cggcaagacc gccgccttcg tcctgccgat cctcgacctg cttcggcagc acgcgaacac
     4081 gtcgttctcg ccggcccgcc atccggtccg tgtcctgatc ctcgtcccga cccgcgagct
     4141 cgcggtccag gtcgacgaaa gcgtccatac gtacggccgg accgtgccgc tccgctcgac
     4201 cgtggtctac ggcgggatgc cgatggagcc ccagatcaag gcgctccgcg gcggggtcga
     4261 gatcctcgtg gccacgcccg gccggctgct cgacctcgtc ggccagaagg tcgccaacct
     4321 cggccaggtc gagatcctcg tcctcgacga ggcggatcgg atgctcgaca tgggcttcct
     4381 gccggacatc cagcgaatca tcgcgctgct cccggcgaag cgccagaacc tgatgttctc
     4441 ggcaacgttc gccgacgaga tccggcgcct gtcgaagacg atcctgaacg acccggtcga
     4501 ggtcgaggtc gccccgcgca acgccaccgt cgacgcgatc caccagctcg tctatccggt
     4561 cgatcgcgac cgcaaggagg agctcctcgc ccacctgatc cgcaaggacg acctccgcca
     4621 gctcctcgtc ttcacccgga cgaagatcgg ggcgacgcgc ctcgcgacgt ggctcgaccg
     4681 gcagggcctc aacgcggtgg ccatccacag cgatcggagc cagccggaac ggacgcgggc
     4741 gctcgaggag ttcaagagcg gcgagatccg ggtcctcgtc gcgaccgacg tcgcggcccg
     4801 cggcctcgac atcgaggacc tcccctacgt cgtgaacttc gagctgccgt ggaaccccca
     4861 ggactacgtc caccggatcg gccggacagg gcgggccggg gccagcggcg aggcgatcag
     4921 cctggtctgc atcgacgaga ccgacctcct gcgcggcgtc cagcgcctcc tccgcaaggc
     4981 aatcccgtgg acggtcgaga acggcttcgt gccggatcgc aacatcgaac cgcgaccgct
     5041 gcgcggcggg gacatgggcg gcagccgcgg cggcgcacga ccgacccatc acccgcaccg
     5101 caagcccgtc cagtaccggg ccggcgccgg ccggtcctag gcgagcggcc cggtcgcttg
     5161 tcccgcgcag tcacgtctcg cgcgactcgc gacggcgccg aagacgactg accgggcgga
     5221 catcgacgcc atcgatgccc gcccgcgagc gacgctgaca acgacccgcg tacgaccgcc
     5281 cgggccgcgt cgcgcgcacg catctcgctc ggcgcgctcc cgatctgacg tgcgccgacc
     5341 gcccgagacg gggttcggac cgacgatgcc cacgaaatgg cgatgggatc gctaccggcg
     5401 cgcggatggt ccagcttggg cagtcccgga acagcgccgt gtgtcgcgtg cgggtcaggt
     5461 ggcggcagtc gaggtcggcg cgaagggacc gctggcgcct ggcgccgtgt cgacggttga
     5521 cgcagcggcg gttgacgcgc cggcggttga cgcgccggcg gcgctccgct cgtgaccgcc
     5581 ggtcacgatc atctccgcgc cagggaagaa gaagccgagc atcaggtcgg cggccacccg
     5641 atcgacgacg tagcggttga cgaacgcggc gccttgggcg accgccgggc catctgcgtc
     5701 cccgaggagg ccgatccgga cgtcccggag gaggacgaac acgccggtcg gccgggcgag
     5761 ggcgcgaccc gggtcgaatc cgggcatcgt cggcatctgg ccggtcacgc ggtagggccc
     5821 gacgtcgagg ccgatatcgt gccactgggc atggaccggg ccatcctcga tctggtcggc
     5881 gaccgcgacg aggatgtcgt cctcggcgat ggccagggcg tcgctcggtc gaggcgtgga
     5941 tccgtcgagc ggatgccagg ccgcccgttc gacgagcagg tcgagaccgc tctcgaggat
     6001 ctcgcggagc cgtccggctc gcgcgacgac gccgacggcc acgcccgcgg cgccgtagat
     6061 ctcgacccgg atcggcattc ccgtccctcc aggcaggatt cgagtctgct ctgcccatcg
     6121 cacgtgcgca tcgcccgtcg gtacctccgc ccatggtccc ttcgtcctgg gactgaaccc
     6181 gggttcgcgt ccggacgcgg aacgcgggcg gccccctgtg gagactgctc gggtcggtca
     6241 gcgggccatc gaacatgtcg gacacaccgg cggacgaccc ggcactggaa ccggcgccgc
     6301 gaccggcagg gcggccgcga ggagcgcccg gacgatcacg cccgggtcgg cccggctctg
     6361 gttcgtgaga acggcgatcg tcaatccgtc gatcgggaag tggcggacgg ccgagcggaa
     6421 gcccaggagg cggccgctgt ggccgaggct cggatggccg tcgatcgaga acgcctggac
     6481 gccgtacccg tagacgacgc cgggcaggta gttcgtcgtc ttcgagaacc cgctcaggag
     6541 cgtcgcggtc ccctgtggcc cgaggacctt gccgccgtac agggcgcgtg cccaccgggc
     6601 gaggtcggtg ctcgtcccgg ccatcgagcc cgccccggcg gccgcggtga tcacggatcg
     6661 gaacggggcg atgccactgc cgtcctcgag atcgatcggc ctcgcggtcc gtttggtgcc
     6721 aacgaagcga tagccgtggg cgaggggcga acgggccgcc tcaccagcct ggtaccaggt
     6781 ggcggcgagc ccgaccggct cgagcaggcg ggtccggatc gcgtcggcca gcgtctggcc
     6841 cgtcgcgcgc tcggcgatca gtcccagcaa gaggtagttc gtgttggcgt agtgccaggc
     6901 ggcacccggc agcgagagag gcttgccgac gtagcggagg gtccgctcag tggtccaggc
     6961 agcggtcggg tgaccctgga gcgggccgtc gatcttcggg ttgaggaagt agtcggcgag
     7021 gccgctcgtg tggttcagca gcatgccgac cgtgatccgc cggtcgatcg tgatccggag
     7081 tggcggcagg aggtgcgcgg cggaatcggt caggtgcatc cgtccctcgc cgatgagctc
     7141 gagcaccagg gcggaggtga aggtcttgct gacgctcgcg aacgcgaacg cggtgtcggg
     7201 cgtgaccctc gttcgcgcgg cgacgtcggc gaagccgctg accccggacc agctcgtccc
     7261 gtccgggagc aggatcgtcg ccgacacgcc cgggatcgcg agcctggcgc ggacgcggtc
     7321 gagcgctgcc tggagtcggg ccgcgaggat ggctgacggg tcgtcgatcg atggacccgg
     7381 cgaggagccg ggaccgggcg acggcgatcc ggcaggcgcc gacggggcca ggggcgcgcc
     7441 cgtcgtcgac ggcgaggcgc cgcccggtgt cgtcgggctg gcgcatgcgg cggacgccac
     7501 ggcgggcgcc cctgcgcacg ggcccgatgt gatcgccgat ccccgcgcga cgccgtccaa
     7561 cggcgctcct gcgaggccgc ccgcggtccg gatgaacccg atgccgagga ccgcgatccc
     7621 ggcggccgcc gcgagcaggg cgccggccat ccgcctccgt cgcggcgggt gcgggcgggg
     7681 tcggcgagcg tcggaggagg gctgcgcggc agggtcggcc atgtgggtgc gacgataccc
     7741 cccaccggtc gaccgcccga cggcaccggt ccaggtggtc cacgtggatg gaggcagcat
     7801 ggttcgatcc ctcgggttcc tcggcgtccg aacgaaggcg ttcgccgaga tggcagcgct
     7861 ggatcgcgac gtcctcgagc tcgagcccga cgatttcgag atcgtcggtc cggatcggag
     7921 cgccgcatga gcgacgagcg aggcgcgacg acgatcgccg ccgacaacgg ggccgcggcc
     7981 ctcttccggg cggtcggcct gatggcggac ggtcccgtcg tctggggcag gcccgtgccc
     8041 gcccggggct ccggggtctt cgtcatcgaa ctgccgacga cgctccaccg tgcgcccatc
     8101 gagcacagcc ggatcggcaa atggctcgag cacgtccccg agctcctgct cgatggcgct
     8161 catccgacca cgcgggcgat ggcggctcgg ctcgaggcct tctggctgcc ctcgacccgg
     8221 gtcctcttcg tcggcaccac ggacggctcg atcggcggtc ggatcgaggc gatccggaac
     8281 actgtcctcg gcgaccgccg gccgcatccc ggaggccact ggctgcatac tctcaccggg
     8341 ctcgaggggg cccgggtctg gtgggcggcg acgtcggccg tcgaggagtc cgaggacgct
     8401 ctcctgaccg cattcgccgc cacgatcccg gtggccgaac aggcggccct ccacgatccg
     8461 acggtcgtcc tgccgttcgc caacctgcgg accgcgaccg gcgaacgcaa gaagaccggg
     8521 ctcgccaact cgctcgtcgc cgccgagccg gtccggccga caccggccgg tcggatcgtc
     8581 gtcgtggcgc cgggcgccgc ggacggagcg agcggcctgc caccgacgcg ggcggccggg
     8641 ggtacggtcc gccgatcgcc ccggccagcc ggctcgacac cggcccgtcc gcgatcgacg
     8701 ccggcgacac cgcgttcgac tgctgcgaaa cgcaagccgg gcgaccgacc cgcggcggag
     8761 acgacgtggg tctcggccga gggcatggaa cggttgaaag ccgagcacgc cgagctgacg
     8821 ggcacgaagc gccacgaggt gatcggtcgg atcaaggccg ccaaggagct cggcgacctc
     8881 aaggagaacg ccgactacgc ctcggcccgc gaggagcagt cgttcctcga gggccggatc
     8941 aagtcgatcg aggcgaccct tcgggtggcc acggtgatcg agacgcccgg cgatgcggtc
     9001 gatcgggtca ccctcggctc gaccgtgacc gtcgaggacg acaccggggc ccagatgacg
     9061 ttcgtcgtcg tcggctcgtc ggaggcggat ccggcggccg gccggatctc gaacgtctca
     9121 ccggtcggcc gggccctcat cgggcgatcg gccggcgacg acgtcgcggt tgcggcgccg
     9181 cgcggcgaag tccattaccg gatcgtcgag ctggcgtgac gaagccccga ccggcgggac
     9241 cgaccgggtc aggccggctc aggccgggag gcggcggccc cggaacaggc cggccaggag
     9301 gaccgccacc ccggcgccga tcaggatgac cgggccgatg tccgtgctca ggaacggcgt
     9361 gccggacagg ccgatcacgc cttcgaagaa gagcccgaat gcgacgaaca gggcaaggcc
     9421 cgagccgagg gatcgcaggc cgttcgtgac gagccccgga tcgcggtgcg tcaggccgta
     9481 gccgatcagg ccgacgcccg tgccgccggg tcccacgagc gcccaggcgt atgcccacgt
     9541 cgcccacagg ccggtcgcgt tctggaccgc gaggatcaac ccgacgacgg tcgtgatccc
     9601 gccggcgacg gccaggctga ccccttcgcg gccggtcatc gtgaggcccc aggcgaagac
     9661 caggacgccc ggaatgatca cgaacagcgg ccagccggca tcggcgaggt cgaacccgac
     9721 cacgcggtcg gcgaggaaca gaccgccgag ggcgatcagg acgatcccga ggatcatgcc
     9781 gccgcggttg tccggcggcc gttctccggt catcgtcccg ggtccatccc gtcggcggtc
     9841 atcgcaggtc gcgggccggt tcggccagct tgtgctcgac cgcccagagt gcggcctgcg
     9901 tccgcgacgc gagcccgagc ttgccgagga tgttcgagac gtaggttcgg gcggttcgct
     9961 cggtgatgaa cagggcggag ccgatctcct tgttgctcat cccccggccg agcagccgga
    10021 tgacgtcctt ctcgcgatcg gtgagcggct cgaccggctc accttcgccg ctcttcctgg
    10081 cccgcatccg ctgggcgagc aggcgagcaa cggcggggtc gagatggacc tcgccggcaa
    10141 aggcggcgcg gaccgcgctg gcgacctcct cagcctcggc atcctttagg acgtagccgg
    10201 acgcgccggc ttcgagggcg gcggtgacct tgtcctcctc gatgaagctg gtcatcgtga
    10261 cgatctcggt ctctggccgc tcggccttga tccggccgat cgcggtgacg ccgtccatgt
    10321 tcggcatcag caggtccatc aggacgacgt ccggctcgag gcgacgggcc atggccacgc
    10381 cctcgctgcc gtccgcggcc tcgccgacga cctcgatgtc gtcctgcagg tcgaagaaga
    10441 cgcgcagtcc catccggacg acggaatggt cgtcgacgat gaggacgcgg atccgggcgt
    10501 tctcctccac gtgacctccg ttgggaatgg gtcgcggact gcgggtcggg gtcgtgctgg
    10561 gcacggtcga gtctagcaag gaacggaccc tcctcaggcc tcaggcgagc gtgcgacggg
    10621 tggtcgcgag gaccgcgtcg atcagctcgt ggggccggaa tgtcttgcgt gcataggcat
    10681 cggcgccgct cgtcaggtcg gccgtctcgg accagttcag gacgaggacg cgggtatcct
    10741 tcgcgacggc gcgaagccgg gcgatcaggg cggtgccgtc gatgcccggc aggcgcggat
    10801 ccacgacgac gacatcgggg gcggtttcgg cggcgaggcg gacggcacca tcggcggtcc
    10861 cggccgtgcc gatgaccagg cacctctgac cgatgcacaa gaggccggcg aggctttcgc
    10921 ggactcggtc gtcggggtcg acgaggagca cgcggagcgg agccggcgct gcagcggggt
    10981 cggtcatgcc gcgcagtgtc ggctggccga caccccggcc gacagggatg gacgtcccga
    11041 atcgggctac gtcattcggc cagttgcggt gggacccggt cgtgggggcc aggccgctca
    11101 gcggcgccgg cggcgcggtc gagcgcctcg ggcgggacga tcacctcgat cgtggtcccc
    11161 tccccggggc gcgagatcac gtgatagctc gccccgatct tgtcggcccg ggcgcgcatc
    11221 cccgccaacc cgaggtggcc gtccggcacg gtggcggcgt cgaagccctt gccgtcgtca
    11281 cggatgcgca gcacgacgtc cgccccacgt cgatcgaccg ccagctcgac ctggcgcgcc
    11341 gcggcgtgct tgacgacgtt gtggagagcc tcctgggcga tccggtacag gacctcctcg
    11401 acctcgagcg gcaagcgctc ggtcagctcg ctcgtcacga cgaccggcag cccgatccgg
    11461 ccctggaggg cggccgtgtg ggtcctcagg gccggcagca ggccgtcgct ctcgatgttg
    11521 cccggccgca gctcgaagat gagggcccgc atctcggcga gggcttcgcg ctggaggtcg
    11581 cggagcgagg tcagcttctc cttggcgacg gctgtgtcgc gatcgatcag gagctccacg
    11641 gaccgcgtca cgagggtcat cgagaacagc gcctgtgtca ccgagtcatg gagctcacgg
    11701 gcgaggtggg cccgctcctg gctcgatgcc agctcaccgg cctggcggcg gaggtcgcgc
    11761 tcgagccgct cgcggacgct gatgtcgcgg gcggcgccgt gaatgccggc gaagcggctc
    11821 gcggcgtcgg tcatgccgat cgcgttgacc tccaccgcta ccgaaccgcc gtcgacccgt
    11881 cgcatccgga agcgctggac ctgcatccgg ctcggctcgt cgatgaacgc ctgccaggct
    11941 gccgcggcat cggggaacgt gtcgaggtcg atgatcgtgg agaaatgctt gccgagcagc
    12001 tccgtcgagg cgaagccggt cagcctctcg atcgtctcgg agtagtacgt atagcggcct
    12061 tcggcgtcgg tcgtgaagat gatgtcgggc gagttctcga cgaggaagcg gtagcgctcc
    12121 tcggactggc ggagctcgcg ctcgagtcgc tcgcgctcgg cgatgtcgcg cgtcgcgccg
    12181 tggatgcccg cgaacacgcc gttgaccgtt gccccgatcg agctgacctc gaccggcacg
    12241 gttccaccgt tcttgtggat gagctcgagc ttcacgacct ggcgttgcga cggctcggcg
    12301 acgatcgcgt cccagcgctc gaccgcggcc ggctgcgtca gcgggtcgac gacggccgag
    12361 aagtgaccgc cgaccagctc tgacggtgag aacccggtca ccttctcgat cgattccgag
    12421 agatacgtga agacgccctc ggcgtcggtc gagaagatga tgtccggcga gttgtcgacg
    12481 aggaagcggt agcgctcctc ggactcgcgc agctcgcgct cgagacggtc gcgctcgctc
    12541 atgtcgcgga cgctgccgtt ggcgccggcg aacacgccgt tctcgtcgcg cgtcgcgaag
    12601 gcgatgaact cggccgggac ggggctgccg tcgcgacgga ggaggttgac ccggccgcgc
    12661 agctcgcggc cgccctcgcc atcacccgcg tcggcggcga tcccggcggt ccagtcgatc
    12721 tcggccacgg cgcgcgacga ctcgtggacg agcgccccga agtgccgccc gagcagctcc
    12781 tcggcccgcc agccggtcag tcgctggcag gtgtccgaga tgaacgtgaa gcgcgcgtcg
    12841 gcgtcgatcg accagacgag gtccggcgag ttctggacga ggtagcggta acgggcctcg
    12901 gactcgccga ggagctcgta gaggttcgcg ttcgtcaggg cgatcgccgc ctggtcggcg
    12961 aggccctgga ggaggtcgag ctcgtcgggc aggatctcgc gcggcttggc gtaggagatc
    13021 gccagcgtcc cgatcacctc gccggccggg gcgcgaagtg gagcggcggc catcgcccgc
    13081 aggccgagcc gcacggcggc ctcgtcgtca ccgggctcgt ggggaatccg cgggtcggcc
    13141 tcgtagtcgg cggtccagat cgggcggacc tcctcggcgg cgagaccgtt gatcccgccc
    13201 ccgagcggga attgcatgtt cttcagccac gactcggtgt cgacgtccat cccgcccgcg
    13261 acgatgaccg ggacgaggaa cgtgccgtct tccgacatcc gggtcaggtg ggcgccgtcc
    13321 gaacggagca ggcggcgcga ctcgtcgacg acgcgggaga ggatctcgcc gggatcgccg
    13381 agcgaggtga tccgggccga gatgtcgcgg agggagcgct ccgtctcggc ccgtgcctcg
    13441 agctcggtct gcgaccgctc cagctcgtcg atcaggctcg cattgatcat cgcgatcgcg
    13501 gcctggtcgg cgagggcccc gaggaggttg gcctggtcct cgccgaaccg gcccggctcc
    13561 ggcgagaaga ccgcgatcgt gccgagggcg ccgcgctccg acaccagggg cgcgatcgcg
    13621 atcgcccgga ggcccaggcc gcgggccacc tcggcgctct cgccatcgac cgcgaaccgc
    13681 tcgtcatcga gatagtcgac ggtgaacagc gccgtccgcc cggccagcgc ccgccggacg
    13741 atgtcctcgc cgacccggct gccggcccag cgatcgatcc ggtccggatc gagg
//
LOCUS       DATAIW010000006         3939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149179, whole genome shotgun sequence.
ACCESSION   DATAIW010000006 DATAIW010000000
VERSION     DATAIW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149179"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="VHM48_00210"
     CDS             complement(<1..1832)
                     /locus_tag="VHM48_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX2753851.1"
                     /translation="MTDPNGRIPLSTAGATSPADAAAYPAPPARPWIRADGQAKATGQ
                     ARYTADLSFPGLAHARLLLAGRVHARIVRLDTSRARAMPGVLAVLTKDDVPERRYGSF
                     DYVLDRTLFARDVVRFEGEVVAAVAALTPEQAAAGVEAIDVEYEDLPPILDVERALEP
                     GTPLVHPAVDTYDHDPNIDPAGNLAGRSTIVKGDATAALAGAPVVVRERYVADMAHPV
                     PIEPHTVTADWSGDRVTIYSSTQVPFFARGKTAEVLEIPEHRIRVVVSHLGGGFGGKC
                     DFHFEGHVAALARAARRPVRLVLSRREEFVAPDKVTHAMVVDLETGLAEDGTILARKA
                     RILLDCGAYASDTPVLGQVAAMMIAGPYRIPDVDIEALSVYTNRTPSGSVRAPTGPQA
                     CWAVEQHHDVLAARVGLDPVEFRRRNLVRDGDEGPTRQRHEGDGAIDTLEQAVARIRS
                     GGPLGPNEAIGVASGWWFSAPGPSGAFVRLESDGSGRIVTGAQENGTGAVMALPILAA
                     EELGMRPEDFAITYQDTDAGPYDGGSSGSQTTFNNGRAVVEAAREVKRQLLELASVAL
                     EAAVADLELRDGHAGVVGSPDRRISIADLAATAHDGALLLARGSG"
     gene            complement(2066..2626)
                     /locus_tag="VHM48_00215"
     CDS             complement(2066..2626)
                     /locus_tag="VHM48_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753852.1"
                     /translation="MKTRRAASTDERQPHPLDRSAARNPSPDDAAGVVDAPDERSGSA
                     GVAGDRIDLTGLSVAGITRRRVGWVAAALVSVWILVVFARQVGDAGAAAGRAAQLAVD
                     NAALAAEVRSLQDEVDLIVRPEYVAIAARGHGLGDDREIPFTLAPSVPAPVDGAPGSA
                     SARLGAHVDRQTPLESWLSLLFGPGS"
     gene            2763..>3939
                     /locus_tag="VHM48_00220"
     CDS             2763..>3939
                     /locus_tag="VHM48_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2753853.1"
                     /translation="MRTYAPAPTEAVLRAILEDEPSLARGVTHHAVLPAREAVWRDHP
                     DWLDPRIRAGLAARGIDRLYTHQAEAIEAVRAGRDVVVVTPTASGKTLCYALPALQAI
                     AEDPAARALFLFPTKALGQDQVAEFAELSAAAGLTISAATYDGDTPAPIRSAIRGAGQ
                     VVVTNPDMLHSAILPHHTKWFQLFEQLRVIVIDELHTYRGVFGSHVANVIRRLLRLCA
                     HYGSKPVIVCCSATIANPAELAAMLTGRTPILVDRNGAPSGERHVLLVEPPLTHVATG
                     ARGSNLTLAHRWAMPFLRAGRQTVVFGRSRVAVEIMLSTLREALRQDLGPRSRIRGYR
                     GGYLPTERRAIEKGLREGEILGVVATNALELGVDIGRLDVSILAGYPGSVAATWQQIG
                     "
BASE COUNT          539 a         1377 c         1474 g          549 t
ORIGIN      
        1 cccgagccgc gagcgagcag cagggcgccg tcgtgggcgg tcgccgcgag atcggcgatc
       61 gagatccgcc ggtccgggct gccgacgacg ccggcgtggc cgtcgcgcaa ctcgagatcg
      121 gcgacggcgg cctcgagggc gaccgaggcg agctcgagca gctgccgctt gacctcgcgg
      181 gcggcctcga cgaccgcccg accgttgttg aacgtggtct ggctgccgga gctgccgccg
      241 tcgtacggcc cggcatcggt gtcctggtag gtgatcgcaa agtcctcggg ccgcatgccg
      301 agctcctcgg cggcgaggat ggggagggcc atgaccgcgc cggtgccatt ctcctgggcc
      361 ccggtcacga tccggccgct gccgtcggac tcgaggcgga cgaacgcgcc cgacggcccc
      421 ggcgcgctga accaccagcc cgacgccacg ccgatcgcct cgttcggtcc gagcggcccg
      481 ccgctgcgga tccgggcgac ggcctgctcg agcgtgtcga tcgcgccatc gccctcgtgc
      541 cgctggcggg tcgggccctc gtcgccgtcg cggacgagat ttcgccgccg gaactcgacg
      601 gggtcgaggc cgacccgggc cgccaggacg tcgtggtgct gctcgaccgc ccagcaggcc
      661 tgtggtccgg tgggcgcccg gaccgagccc gacggcgtcc ggttcgtgta gacgctgagg
      721 gcctcgatgt cgacgtcggg gatccggtac ggcccggcga tcatcatcgc cgcgacctgg
      781 ccgaggaccg gcgtgtcgct ggcgtaggcg ccgcagtcga ggaggatcct ggccttgcgg
      841 gcgaggatcg ttccgtcctc ggccaggccg gtctcgagat cgacgaccat cgcgtgggtc
      901 accttgtcgg gggcgacgaa ctcctcgcgc cggctcagga cgagacggac cgggcggcgg
      961 gcggcccggg cgagggccgc gacgtgaccc tcgaaatgga agtcgcactt gccgccgaat
     1021 ccgccaccga ggtggctcac gacgacccgg atccggtgtt ccgggatctc gaggacctcg
     1081 gcggtcttgc cgcgggcgaa gaacggcacc tgggtcgacg agtagatcgt cacgcgatcg
     1141 ccggaccagt cggcggtcac cgtgtgaggc tcgatcggga cggggtgggc catgtcggcg
     1201 acgtagcgtt cgcggacgac gaccggcgcg ccggccaggg ccgcggtggc atcgcccttg
     1261 acgatcgtcg agcggccggc gaggttgccc gccggatcga tgttcggatc gtggtcgtac
     1321 gtgtcgacgg cgggatggac gagcggcgtt ccgggctcga gggcccgttc gacatcgagg
     1381 atcggcggga ggtcctcgta ctcgacgtcg atcgcttcga caccggcggc ggcctgttcc
     1441 ggagtgagcg ccgccaccgc ggcgacgacc tcgccttcga agcggacgac gtcgcgggcg
     1501 aagagcgtcc gatcgaggac gtagtcgaac gagccgtacc gtcgttcggg cacgtcgtcc
     1561 ttggtcagga cggcgaggac gccgggcatg gcacgggccc ggctggtgtc gagccggacg
     1621 atccgggcgt gcacccggcc ggcgagcagc aggcgagcgt gggcgaggcc cgggaagctc
     1681 aggtcggcgg tgtagcgggc ctgaccggtg gccttggcct gcccgtcggc acgaatccat
     1741 ggcctggcgg gaggcgctgg atacgcggcg gcgtcggcgg gcgaggtcgc accggccgtt
     1801 gacagcggga tgcgcccatt cggatcggtc acgagcgcct cgtcctcctc gtcgcctgat
     1861 gatcggccga ggatacgtgc tggaccggtg cgtggcggag ccggaccgta gcgcggatcg
     1921 tgtcggacgg cgccggccac cgattctggc gtggccgggc agtcgacgac ggccgtgacc
     1981 ggccggggac gcaggccagg tcagcggcca tgtccggcat cgccggctcg gtgtccggtc
     2041 gccgaccgct gctacagacg gcgggtcagg atcccggacc gaagagaagc gacagccacg
     2101 actcgagcgg ggtctggcga tcgacgtggg caccgaggcg ggcggacgcc gagcccggcg
     2161 cgccgtcgac cggtgcggga acggatggtg cgagggtgaa tgggatctcg cggtcgtcgc
     2221 ccagcccatg gccgcgggcg gcgatcgcga catactcggg gcggacgatc aggtcgactt
     2281 cgtcctggag ggaccggacc tcggcggcaa gggccgcgtt gtccacggcg agctgggcgg
     2341 cgcggccggc cgcggcgccg gcatccccga cctggcgggc aaagaccacc aggatccaga
     2401 ccgagaccag cgcggcagcg acccagccga cccgacggcg ggtgataccc gcgaccgaga
     2461 ggccggtcag gtcgatccgg tcgccggcca ccccggccga tccggatcgc tcgtcgggcg
     2521 cgtccacgac gccggccgca tcgtcgggcg atgggttgcg agcggcggac cgatcgaggg
     2581 gatggggctg gcgctcgtcg gtcgaagcgg cgcggcgggt cttcacgtgc ggtggagggt
     2641 agctgcccgc agctgacatt gtcaactcgc cggggtcgtg acagccaacc tgtcacgaac
     2701 gtcatcgacc ctcctgctag actgccgttc tttcgcacgc atgttcgatt tcgaggtgac
     2761 cgatgcggac ctatgccccg gcccccaccg aggccgtcct gcgggcgatc ctcgaggacg
     2821 agccctcgct tgcccggggg gtgacccatc atgccgtgct gccggctcgt gaggccgtct
     2881 ggcgggatca cccggattgg ctcgatccgc ggatccgggc cggcctggcg gcgcgcggca
     2941 tcgaccggct gtacacccac caggcggagg ccatcgaggc cgtccgggcc ggccgcgacg
     3001 tcgtcgtcgt cacgccgacg gcctccggca agaccctctg ctacgcgctg ccggcactgc
     3061 aggcgatcgc cgaggatccc gcggccaggg ccctgttcct gttcccgacc aaggccctcg
     3121 gccaggacca ggtggccgag ttcgccgagc tgtcggcggc cgccggcctg acgatctcgg
     3181 cggccaccta cgatggcgac acccccgccc cgatccggtc ggcgatccgc ggcgccggtc
     3241 aggtcgtcgt cacgaacccg gacatgctcc actccgcgat cctcccccac cacacgaagt
     3301 ggttccagct gttcgagcag ctccgggtca tcgtgatcga cgagctccac acctatcgcg
     3361 gggtgttcgg gagccacgtc gcgaacgtga tccggcggct tctccggctg tgcgcccact
     3421 acggcagcaa gcccgtgatc gtctgctgct cggcgacgat cgcgaacccg gccgagctgg
     3481 cggcgatgct caccgggcgg acgccgatcc tcgtcgaccg caacggggca ccgtcgggcg
     3541 agcgccacgt gctgctcgtc gaaccgcccc tgacccacgt cgcgaccgga gcacgcggct
     3601 cgaacctgac cctggcccac cgctgggcga tgccgttcct ccgagccggc cgccagactg
     3661 tcgtgttcgg ccggagccgt gtggcggtcg agatcatgct gtcgacgctc cgtgaggcac
     3721 ttcgccagga cctagggccg cgatcgcgca tccgcggcta ccgtggcggc tacctgccga
     3781 cggagcggcg agccatcgag aagggcctcc gcgagggcga gatcctcggc gtcgtcgcaa
     3841 cgaacgccct cgagctcggc gtcgatatcg gccggctgga cgtctcgatc ctcgccggct
     3901 atcccggctc ggtcgcagcg acatggcagc agatcggac
//
LOCUS       DATAIW010000007         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_59799, whole genome shotgun sequence.
ACCESSION   DATAIW010000007 DATAIW010000000
VERSION     DATAIW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59799"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1823)
                     /gene="serA"
                     /locus_tag="VHM48_00225"
     CDS             complement(237..1823)
                     /gene="serA"
                     /locus_tag="VHM48_00225"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX2753854.1"
                     /translation="MRILVVEPIAHEGIERLRAVHDVDERPGISPAELCAILPGYDAL
                     VVRSQVQVDAAVIAAAGNRLQVIGRAGVGVDNVDLDAATAAGITVVNAPTGNTIAAAE
                     HTLALLYGVARKVAAADASVRRGEWKRAQFTGLELRGRTLGIVGLGKIGQAIAARAVA
                     MEMVVLASDPFVTAEQAAHHGVELVSFDALIERSDVITVHVPLTRTTRGLIGRDQIAR
                     MKRGAIVLNVARGGILDEAAVAEALAEGQLGGAGVDVFDTEPPTGSPLLDAPNTLLTP
                     HLGASTAEAQVLVAEEVADQVLDVLAGRSARYAVNAPLLTPETARAIAPYLPLAEMLG
                     RFFAQFARSGVRTVTLEIAGELAAFDASPLTAAVLRGMIETSTTQRVNLVNAAALAKA
                     RGITVVERKTPDAGSYAAQISLSGESGGRTTTVAGTVAGGELRLTRLDDYRLDMGPTD
                     VMLITHHRDHPGMIGRIGQTLGEADVNISAMHVGRSAPRADALMILALDDDVGPEVAE
                     SIRAHDSVIELWTIRLGRDR"
     gene            complement(1820..3031)
                     /locus_tag="VHM48_00230"
     CDS             complement(1820..3031)
                     /locus_tag="VHM48_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX2753855.1"
                     /translation="MDRFEPNLRIPGPTSLPPSVREAGGRQMINHRGPEFAAMLGRVI
                     DGMKPFFGTTNDVVVLTCAGSGGLEAAIVNTLSPGDRVLGVSIGSFGDRFAKIAGIYG
                     AALTKIEVDWGQAADPQVVREALRANPGYRAVLLTHNETSTGVMNPIRELAAAVREAS
                     PDSLILVDSVSALGAVPFEMDEWGVDLVVTGSQKAWMAAPGLAMIALSERGWTAAETA
                     TMPRFYLDVRSHRESAQAGQTPFTPAIGVVFQVDEGLRLMHAEGKEHVFARHEACAAA
                     ARAGLTALGFELFADPRAASKTVTAARLPEGHDWKAFNGAIKSHGVILAGGQGKLAGK
                     IFRLGHLGSVTVEEILGVIAVLERVAVEQGRRIEPGRAVGAAQAAALAVQTGSSAAPD
                     ARQADRATVPA"
     gene            3439..>3674
                     /locus_tag="VHM48_00235"
     CDS             3439..>3674
                     /locus_tag="VHM48_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753856.1"
                     /translation="MDFRHSRVSPAYPRLRTARGRSRRQKALLAILLVGLVGGVLGSA
                     SIATNAFGAGRLYERFVAKVDRFLAGPPPPDRIT"
BASE COUNT          494 a         1320 c         1346 g          514 t
ORIGIN      
        1 ggcatgcggc cgggcgaggc ggcgaccgac gagctcggcc tgccggcgac cggtggcgga
       61 cagcggcgac tcggactgac cctggaatcg gcgctggacg atcagctccg actcgccgtg
      121 gcggacgagg acgagcgtgg cgtcgaggtc ggcgggcatc gcgacggccg aggattcgcc
      181 gtcggcgtcc gcccggctcg gcgcggggcc gccgccgtcg accgcgcggc cgcccgtcag
      241 cggtcccgac ccagccggat cgtccacagc tcgatcaccg aatcgtgggc gcggatcgat
      301 tcggccacct cgggcccgac gtcatcgtcg agggccagga tcatcagggc gtcggctcgc
      361 ggtgcgctcc gcccgacgtg cattgcgctg atgttcacgt cggcctcgcc gagcgtctgc
      421 ccgatccggc cgatcatccc cgggtggtcg cggtggtggg tgatcagcat gacgtcggtc
      481 ggccccatgt cgagccggta gtcgtcgagc cgggtgaggc gcagctcgcc cccggcgacg
      541 gtcccggcga ccgtcgtcgt ccggccgccg ctctcaccgg acaggctgat ctgggcggcg
      601 tacgagccgg cgtcgggggt cttccgttcc accaccgtga tcccgcgggc cttcgccagg
      661 gcagcggcat tgacgaggtt cacccgctgg gtggtggatg tctcgatcat cccgcggagg
      721 accgccgcgg tcagcggcga cgcgtcgaag gccgcgagct cgccggcgat ctcgagggtg
      781 acggtccgga cgccgctcct tgcgaactgg gcgaagaacc ggccgagcat ctcggcgagc
      841 ggcaggtacg gcgcgatcgc gcgggcggtc tcgggcgtga ggagcggggc gttgaccgcg
      901 tagcgggccg aacgcccggc gaggacgtcg aggacctggt cggcaacctc ctcggcaacg
      961 aggacctggg cctcggcggt cgatgcgccg agatgcggcg tgagcagggt gttcggtgcg
     1021 tcgaggaggg gtgagcccgt cggcggctcg gtgtcgaaga cgtccactcc ggccccgccc
     1081 agctgcccct ccgccagggc ttccgcgacc gcggcctcgt cgaggatccc gccgcgggcg
     1141 acgttgagca cgatcgcccc gcgcttcatc cgcgcgatct ggtcccggcc gatcaggccg
     1201 cgcgtcgtcc gcgtcaacgg gacgtggacg gtgatcacgt cggaccgttc gatgagcgcg
     1261 tcgaacgaga cgagctcgac gccgtgatgg gccgcctgct cggccgtcac gaacgggtcg
     1321 gaggcgagga ccaccatctc catggcgacg gcccgggccg cgatggcctg gccgatcttg
     1381 ccgaggccga cgatcccgag ggtccggccg cgcagctcga gcccggtgaa ctgggcgcgc
     1441 ttccattcgc cgcgccggac cgacgcgtcc gccgcggcga ccttgcgggc gaccccgtag
     1501 aggagggcga gggtgtgctc ggccgcggcg atcgtgttgc ccgtcggggc gttgacgacc
     1561 gtgatgccgg ccgcggtcgc ggcgtcgaga tcgacattgt cgacaccgac ccctgcccgg
     1621 ccgatgacct ggaggcgatt gccggccgcg gcgatgaccg cggcgtcgac ctggacctgg
     1681 ctgcggacga cgagcgcgtc gtagccgggc aggatcgcgc agagctcggc gggggagatc
     1741 cccggccgct catcgacgtc gtggaccgcc cgcagccgct cgatgccttc gtgggcgatc
     1801 ggctcgacga cgaggatcct cacgcgggaa cggtggcgcg gtccgcctgg cgcgcgtccg
     1861 gagccgccga cgaccccgtc tggacggcca gagccgcggc ctgggcggcg ccgacggccc
     1921 ggcccggctc gatccgccgg ccctgctcga ccgcgacccg ctccaggacg gcgatgacgc
     1981 caaggatctc ctcgaccgtc acggagccga ggtggccgag ccggaagatc ttgccggcca
     2041 gcttgccctg cccgccggcg aggatgacgc cgtggctctt gatcgctccg ttgaacgcct
     2101 tccagtcgtg gccctcgggg agcctggcgg ccgtcaccgt cttcgatgcc gcccgtggat
     2161 ccgcgaacag ctcgaagcca agcgccgtga gtccggcccg cgctgccgcg gcgcacgcct
     2221 catgacgggc gaagacgtgc tccttgccct cggcgtgcat caggcgcagg ccctcgtcga
     2281 cctggaagac gacgccgatg gccggcgtga acggagtctg gccggcctgc gccgactcgc
     2341 ggtgggagcg aacgtcgaga tagaaccgcg gcatcgtcgc ggtctccgcg gccgtccagc
     2401 cgcgctccga cagcgcgatc atcgccaggc ccggcgcggc catccacgcc ttctgcgagc
     2461 ccgtcaccac gagatccacg ccccactcgt ccatctcgaa cggaacagcc ccgagcgcgg
     2521 agacactgtc gacgaggatc aggctgtcgg gggaggcctc gcggacggcg gcggcgagct
     2581 cccggatcgg gttcatcacc ccggtggacg tctcgttgtg ggtcagcagg accgcccggt
     2641 agccggggtt cgcccggagg gcctcgcgga cgacctgcgg atcggccgcc tgtccccagt
     2701 cgacctcgat cttcgtcagc gcggcgccgt agatccccgc gatcttcgcg aaccggtccc
     2761 cgaagctgcc gatcgagacc ccgagcaccc ggtcgcccgg cgagagggtg ttgacgatcg
     2821 cggcctcgag cccgccggaa ccggcgcagg tgaggacgac gacgtcgttg gtcgtcccga
     2881 agaacggctt catcccgtcg atcacccggc cgagcatcgc cgcgaactcc ggaccgcggt
     2941 gattgatcat ctggcgaccg ccggcctcgc ggacggacgg tggcagcgac gtcgggccgg
     3001 ggatcctgag gttgggttcg aagcgatcca cggggagcct cctcggggcg cgggttggca
     3061 aggcggggtg cagtcgatga cggtcccccg atggtcgcag aagcgctcgg atcgcgccag
     3121 tcgagccgga tcgtcacccg acgacgtggc cgatcttgac ctcgtcacga gaccgtctgc
     3181 tgggcggagc cgttcagcgc gccggactgg cgctcgagct gggttcgccg cgccgacgag
     3241 ccggcctgac aacggccggc ccgttgatcg tcggcggggc gaccgccggg gttcatctcg
     3301 ccaagaggtt cgatctgaca aaggccgtta cgatcacctg catccgctgg tttccgccgc
     3361 gcgtcgcagg cggacgccac cgatcgtcca agtccgcccg accaacgacc gcccgatcaa
     3421 cgcacgaggt gcctccgctt ggatttccgc cacagtcgcg tttcgcccgc ctacccgcgc
     3481 ctcaggaccg cgcgcgggcg gagccggagg cagaaggcgc tgctcgcgat cctcctcgtc
     3541 gggctcgtcg gcggcgtcct cggctcggcg tcgatcgcga ccaacgcctt cggggccggc
     3601 cgcctgtacg agcggttcgt cgccaaggtc gatcgatttc tcgccggccc gccgccgccg
     3661 gaccggatca ccga
//
LOCUS       DATAIW010000008         6419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_30050, whole genome shotgun sequence.
ACCESSION   DATAIW010000008 DATAIW010000000
VERSION     DATAIW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30050"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..763
                     /locus_tag="VHM48_00240"
     CDS             185..763
                     /locus_tag="VHM48_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753857.1"
                     /translation="MRRTRPILVVIALAIGALGGAQSASARSYVDPTTLTPPLKAFRV
                     CWQLGPEVQCDTSGVTSFENLPTDMSPCGQIYETSVEDSNSTRWYRDGLIVRRAVQES
                     VRGTWSLSPTGDGPTIDFMRNLSWDELFAVPGDIDSGVVSVRGSVLRVPSLGSDLHES
                     GLTVPDETDEIHHGLISYTDEGAARLCALLVP"
     gene            794..1354
                     /locus_tag="VHM48_00245"
     CDS             794..1354
                     /locus_tag="VHM48_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2753858.1"
                     /translation="MTARTTAENANELQAALGSDAAFEAWYRRSLPRVFSYVLSRCGN
                     DVTLAEELSQQTFVAAIGQRARFDGRSDSVTWLCGIARHKLADHFRALEREERRRMHL
                     EVRQIQLSDSDLHGPGLDDRAAIGDALRSLPAAQRAVLAFVVLDDLPVAAAARLMGKS
                     TAATQSLLHRARESFRQAYLGESDDD"
     gene            1347..2663
                     /locus_tag="VHM48_00250"
     CDS             1347..2663
                     /locus_tag="VHM48_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753859.1"
                     /translation="MTDDRVDALIRRLDVPSSPPAGFVDSGATALLALARATRAQDAS
                     RIGRLRRDLGSAWQSIAATIRPNARARSGMAWALLIVTLLLAAALVAVLAGSHRRLPP
                     PFGLAGNGRIAFMADGHVFTSALDGADRQQLTFGDGTELAPTFSPDGSRIAYRQFGPD
                     EPAGDPQLADVVVADADGRHPVVIDHAARGLSNISWSPDSRFVAYSRAAPPGNDRAFV
                     TPAGGSSQPHDLGAFSDGAWGPTWAPDGERLAVAAERGLFVVNRDGTDPRKVTHGSYL
                     EVGEKGEAAEWSPDGQSIVFSAGNPDGPTQVYLVGLDGAPERQISADNWSANGGTWSP
                     DGSRIAYMRSGTGTGPVAVITDAAGREIRQLTGEYGWYQPTWSPDGSSLIVTDDRPGA
                     DNVAGPAIRVILDVAGIADPVVIPAAGVTPDMLPDWAASWQRIALP"
     gene            2795..5698
                     /locus_tag="VHM48_00255"
     CDS             2795..5698
                     /locus_tag="VHM48_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2753860.1"
                     /translation="MEFAVLGPLDVRDAGASVAIPPGGAGALLAHLLIRPNRVISAEE
                     LIDAIWGEDAPRTARNVLQAHVAHLRRVLRAGGPGAAERLETRGAGYRIRVEAGEFDA
                     DVFAAEVTEVRRRQRANPVAAADLLRHALGLWRGSAFEDVDIRAGSDSGVIDRLEDFR
                     LGALETRSELDLRLGRVADVIGDLERLVAEHPFRERFHGLLMLALYRAGRQADALDAY
                     HRARAVLDEELGVEPGPELRALQLAILRQDRELMADDSRIGALVNGDDDGTGADEATD
                     GRPHATAVPNNLPVELTPFIGRDRELAGLLALLGEERLVTVTGVGGTGKTRFARQAAS
                     LALDSFPGGAWFIDLAPVPDGDRILPTLAAAVGVPEQGGRSLFDTVVGAVGQSRLLLV
                     IDNCEHVIDASARLIDGMLRAVPGLVVLATSREPLRLSSEVLLRLVPLPVSAADGLPR
                     AELLATDAVRLFLDRAGRVRSIDQLSDADVATIAQICRRLDGLPLALEMAAARLRVLS
                     PSDILDRLEDRFSLLTDGGRIAAERHQTIRATLDWSHDMLDEAERATLRRMSSFVSAV
                     PAAAVEGICAGEGVGDALDSAMRLADRSLVVANVGADGRTRFGLLDTVRDYGRVHLRA
                     SGEETTTLLRHVGWWTSIAERAYALRFADPDGQAAELEAVLGEIRVALDRAHALAPAG
                     ETHLAGLLGWFWAAHSHLVEGRVLLDRALSVDGADEVDRARALAARGSIASFQGDGTA
                     ADDLAAALALWRGRGDRVEECATLDTRGWARFFSGDVDGAGSDFEAGLLIATEVGDSA
                     LVRRTSAGLCQILVARHDVERARPLAEEILRIASDDIRTAHFGHHFLADCDLMSADCV
                     PARDGYRRSLVLAERMQDGLEVALEIEGVGMASAGCGEPELALRLGGAAAAILERLEV
                     DVDVPFWAAFEKLWFGLAREALGPAADAAWDAGRTMSMADAIREALAAAPVPA"
     gene            5794..6231
                     /locus_tag="VHM48_00260"
     CDS             5794..6231
                     /locus_tag="VHM48_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2753861.1"
                     /translation="MADQDLVTVADRWHQDIYLRGRLEAADEICAPDMVAHGTGVAAD
                     APTGPAFVREDAAGLREAFDIESLTNDDVVIAGDRVVIRWSMRGRHVGPFLGVDGTGR
                     RVAFTGIDIFRLERGRIAEFWGEFNLLDLAQQVGALTAELTTV"
BASE COUNT          854 a         2306 c         2303 g          956 t
ORIGIN      
        1 gtctggtgtc gcggcggccc ggaaaagtgc cgagtgtccc gattcggcga ggtgatgctc
       61 gaggagccgc ttcgggcact cgggatcgcc ccagacttcg tcgcatcgaa tgattccctg
      121 cctcccgagc gactcacctg caatggccga acgcatcggc cgaggcggta tagggaggct
      181 cgcgatgcgc agaacccgtc ccatcctcgt cgtgatcgcg ctggcgatcg gagcgctcgg
      241 cggtgcccag agcgcatccg cgcggtcgta cgtcgacccg acgacgttga cgccgccgct
      301 caaggcattc cgggtgtgct ggcagctcgg gccggaggtc cagtgcgaca cgagcggcgt
      361 cacctcgttc gagaacctcc cgacggacat gtcgccgtgt ggccagatct acgagacgtc
      421 ggtcgaggac agcaactcga cgcgctggta tcgcgacggc ctgatcgtgc gtcgggccgt
      481 gcaggagagc gtgcgcggca cgtggtccct gtccccgacg ggcgacggtc cgacgatcga
      541 cttcatgcgg aacctgagct gggacgagct cttcgcggtc ccgggcgata tcgacagcgg
      601 cgtggtcagc gtccgcgggt cggtgctgcg cgtgccctcc ctcggctccg atctccacga
      661 gtccgggctg accgtccccg acgagacgga tgagatccat cacggcctga tctcgtacac
      721 cgatgaaggg gcggcgcggc tctgcgccct gctcgtaccc tagatcgccg caccgacgag
      781 gagcgcgacg gagatgaccg cccggaccac ggcggagaac gccaacgagc tgcaggctgc
      841 gctcggaagc gacgccgcgt tcgaggcgtg gtaccggcgg tctctccctc gcgtcttcag
      901 ctatgtcctc agccgttgtg gcaacgatgt cacgctggcc gaggagctga gccagcagac
      961 gttcgtcgct gcgatcgggc agcgggcccg gttcgacggt cgctccgatt ccgtcacgtg
     1021 gctgtgcggc atcgcccggc acaagctcgc cgatcacttc cgcgcgctgg agcgagagga
     1081 gcgtcgacgg atgcacctcg aggtccgcca gatccagctg tcggacagcg atctccacgg
     1141 gcctgggctg gatgaccggg cggcgatcgg cgacgcgctt cgctccctgc ccgcggccca
     1201 gcgagcggtt ctcgcgttcg tcgtgctcga cgatctgccg gtcgccgccg ccgcccggct
     1261 gatgggcaag agcacggcgg cgacgcaatc gctgctccac cgcgcccgcg agagtttccg
     1321 gcaggcatat ctcggggaat ccgacgatga ctgacgaccg cgtcgacgcc ctgatccgac
     1381 ggctcgacgt gccgtcgagc ccgcccgccg gcttcgtcga ttccggcgcg acggcgctcc
     1441 ttgccctcgc ccgcgccacc cgtgcccagg acgcgagccg gatcggtcgg ctccgacggg
     1501 atctcggcag cgcctggcaa tcgatcgcgg cgacgatccg gccgaacgcg agggcccggt
     1561 cggggatggc ttgggcgctg ctcatcgtca cgctgctcct ggccgccgcc ctggtggcgg
     1621 tcctcgccgg atcccaccgt cggctgccgc cgcccttcgg cctcgccgga aacggccgga
     1681 tcgcgttcat ggccgacggc cacgtcttca cgtccgcgct cgacggagct gatcggcagc
     1741 aactgacctt cggcgacggg acagagctcg cgccgacgtt ctcgcccgac ggctcccgga
     1801 tcgcctaccg ccagttcggg ccggacgagc cagctgggga tccccagctg gccgacgtcg
     1861 tggtcgccga tgccgacggc cgccatcccg tggtcatcga ccatgcggcc cgtggattga
     1921 gcaacatctc ctggtcaccg gacagccggt tcgtcgcgta ttcccgagcc gcgccgcccg
     1981 gcaacgatcg cgcgttcgtg acgccagcag gcggatcgag ccagccacac gatctcggtg
     2041 ctttcagcga cggcgcctgg ggcccgacct gggcgcccga cggcgagcgg ctcgcggtcg
     2101 ccgccgagcg gggcttgttc gtcgtgaacc gcgacggcac cgatccgcgc aaagtcaccc
     2161 acggctccta tctcgaggtc ggcgagaagg gcgaggctgc ggagtggagc cccgacgggc
     2221 agtcgatcgt gttctcggcc gggaatccgg acgggccgac acaggtctat ctggtcgggc
     2281 tcgacggcgc acccgagcga cagatctcgg ccgacaactg gtcggcgaac ggagggacgt
     2341 ggtcgcctga cgggtcgcgc atcgcctaca tgcgatcggg caccggcacg ggaccggtcg
     2401 cggtcatcac ggacgcggcg ggacgcgaga tccgccagct gacgggcgaa tacggctggt
     2461 accagccgac ctggtcgccg gacgggagca gcctgatcgt gaccgacgat cggccgggag
     2521 ccgacaacgt cgcaggcccg gcgatccggg tcatcctcga tgtcgcgggc atcgcggatc
     2581 cggtcgtgat cccggcagcc ggcgtgaccc cggacatgct tccggactgg gcggcgagct
     2641 ggcagcgcat cgctctgccc tgagggaacg tcagcgagac ccgccggatt gacagcgcaa
     2701 cgggactcga tgatcctcgg agcgtcgccg gcggcggcgc gcagatcccg tcacctgccg
     2761 ggccgtcggg ggtccaccgg ctcttcggag gcccatggag ttcgcggtcc tcggtccact
     2821 cgacgtccgg gatgccggcg catccgtcgc cattccgccg ggtggcgccg gggcgctgct
     2881 ggcgcatctc ctgatccgcc ccaatcgggt catctcggcc gaggagctga tcgacgcgat
     2941 ctggggtgag gacgctcctc gcaccgctcg caacgtcctc caggcgcacg tcgcccatct
     3001 ccgtcgcgtg ctccgtgccg gcggacccgg cgctgccgaa cggctcgaga cgcgcggggc
     3061 cgggtatcga atccgggtcg aagcgggtga gttcgatgcc gacgtcttcg ccgccgaggt
     3121 gaccgaggtg cggcggcgcc agcgggccaa tccggttgcc gcggcggatc tcctccgtca
     3181 tgcgctcggg ctgtggcgcg gctcggcgtt cgaggacgtc gacatccggg ccggatccga
     3241 cagtggagtc atcgaccggc tcgaggactt ccgcctcggt gccctcgaga cgcggtcgga
     3301 gctcgacctc cgactcggcc gcgtcgccga cgtcatcggc gatctcgagc ggctcgtcgc
     3361 cgagcatcca ttccgggagc gctttcacgg cctgttgatg ctcgccctgt accgagcggg
     3421 ccggcaggcc gacgccctcg atgcgtatca ccgcgcgcgt gccgtcctcg acgaggagct
     3481 cggcgtcgag cccgggccgg agctgcgcgc cctccagctg gcgatcctgc gccaggaccg
     3541 cgagctcatg gcggatgact cgcggatcgg cgcgctcgtc aacggcgacg acgacgggac
     3601 cggcgccgac gaggcgaccg atggccgtcc ccacgcgacc gccgtcccga ataacctgcc
     3661 cgtcgagctg acgccgttca tcggccgcga ccgggagctc gccggcctgc tcgcgctcct
     3721 cggcgaggag cggctcgtga ccgtgaccgg cgtcgggggc accgggaaga cgcgcttcgc
     3781 ccggcaggcc gcctcgctcg ccctcgactc gttccccggc ggagcctggt tcatcgatct
     3841 cgcccccgtc cccgacggcg accggatcct gccgaccctt gcggcggcgg tcggcgttcc
     3901 cgagcagggc ggccggagcc tgttcgacac ggtcgtcgga gcggtcgggc agagccggct
     3961 gctcctcgtg atcgacaact gcgagcacgt gatcgatgcg agcgcccggc tgatcgacgg
     4021 gatgctccgg gcggtcccgg gcctggtcgt cctcgccacc agccgcgagc cgctccggct
     4081 gtcgagcgag gtcctcctgc gactcgtgcc gctcccggtg tcggccgccg acggactccc
     4141 gcgggccgag ctcctggcaa cggacgcggt ccgcttgttc ctcgatcggg ccggccgggt
     4201 gcgctcgatc gatcagctca gcgacgcgga cgtggcgacg atcgcccaga tctgccgccg
     4261 cctcgacggc ctgccgctgg cgctcgagat ggcggcggcg cggctgcggg tcctgtcgcc
     4321 gagcgacatc ctcgaccggc tcgaggatcg attcagcctc ctcaccgacg gcgggcggat
     4381 cgcggcggag cggcaccaga cgatccgggc gacgctcgac tggagccacg acatgctcga
     4441 tgaggcggaa cgggcgacgc tccgccggat gtcgtcgttc gtgtcggcgg tgccggccgc
     4501 ggccgtcgag gggatctgcg cgggcgaggg tgtcggggac gccctcgact cagcgatgcg
     4561 cctcgccgac cggtcgctcg tcgtcgccaa tgtcggcgcc gacggccgga cgcggttcgg
     4621 gctgctcgac acggtccgcg actacgggcg cgtccacctg cgtgcgtccg gcgaggagac
     4681 cacgacgctg ctccgccacg tcggctggtg gacctcgatc gcagagcgtg cctacgcgct
     4741 gcggttcgcg gatccggacg gccaggcggc cgagctggag gcggtcctcg gcgagatccg
     4801 ggtcgcgctc gatcgcgccc acgccctcgc gcccgccggc gagacgcacc tggccggcct
     4861 gctcggctgg ttctgggcgg cgcacagcca cctggtcgag ggccgggttc tcctcgaccg
     4921 ggccctgtcg gtcgacgggg cggacgaggt cgatcgggcg cgagccctgg ccgcgcgcgg
     4981 ctcgatcgcg tccttccagg gcgacgggac ggccgcggac gatctggcgg cggcgttggc
     5041 gctctggcgc gggcggggcg accgggtcga ggagtgcgcg acactcgaca cccgcggctg
     5101 ggcccgcttc ttctcgggcg acgtcgatgg tgcgggcagc gacttcgagg cggggcttct
     5161 catcgcgacc gaggtcggcg attctgccct cgtccggcgg acgagcgccg ggctctgcca
     5221 gatcctcgtc gcccggcacg acgtcgagcg agcccgtccg ctcgcggagg agatcctccg
     5281 gatcgcgagc gacgacatcc ggaccgccca cttcggccac cacttcctgg ccgattgcga
     5341 cctcatgagc gccgactgcg tcccggcacg ggacggctac cggcgcagcc tcgtcctcgc
     5401 ggagcggatg caggacggcc tcgaggtggc gctcgagatc gagggcgtcg ggatggcgtc
     5461 ggccggctgt ggcgagccag agctcgcgct tcggctgggc ggagcggcgg cggcgatcct
     5521 cgagcggctc gaggtcgatg tcgacgtgcc gttctgggcg gcgttcgaga agctctggtt
     5581 cgggctcgcg cgggaggccc tcggtcccgc ggcggatgcc gcctgggacg cgggtcgcac
     5641 gatgtcgatg gcggatgcca tccgcgaagc gctggcggcg gcgcccgtcc ccgcgtaatt
     5701 cagcacgatt cagcgggcgt cagcgcgcat tcagcagtcc tcgccatcgt ggtccccaga
     5761 cgtcgcacct gcgaccacga tggaggacag gacatggccg atcaggacct ggtcacagtc
     5821 gccgaccgct ggcaccagga catctatctg cgcggccggc tcgaggcggc ggacgagatc
     5881 tgcgccccgg acatggtcgc ccacgggacc ggcgtcgccg ccgacgcccc gacaggaccg
     5941 gcattcgtcc gcgaggacgc cgccgggctc cgcgaggcgt tcgacatcga gtcgctcacc
     6001 aatgacgacg tcgtcatcgc gggcgaccgg gtcgtcatcc ggtggtcgat gcgcggccgg
     6061 cacgtcgggc cgttcctcgg cgtcgacggc accgggcgac gggtcgcctt cacgggcatc
     6121 gacatcttcc ggctggagcg tggccgcatc gccgagttct ggggcgagtt caacctgctc
     6181 gatctcgccc agcaggtcgg cgcgctgacc gccgagctga cgaccgtcta gccggctcgt
     6241 ccggccaacc ctgatcaccc tacaagagga gcattccgat gaaccgcatc ctgcgcaccg
     6301 tcattctcgt cgtcgccctg gcggcccttg cgccgtccgt tgcggccgcc gcgtcgccgt
     6361 cggcctcggg cacctgggac gattgcaact tcgcaccgat cgtcagcacc gccggtccg
//
LOCUS       DATAIW010000009         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_89621, whole genome shotgun sequence.
ACCESSION   DATAIW010000009 DATAIW010000000
VERSION     DATAIW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89621"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHM48_00265"
     CDS             complement(<1..278)
                     /locus_tag="VHM48_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753862.1"
                     /translation="MTGTLQDVVLTRPAPTADAGPLDERFYDLVEARFRRIVAENPLV
                     GTYLGIHTEDHRLGDGTRDAVLAELAAEKAHLAAVEAIDVEGLSPDARL"
     gene            complement(545..1693)
                     /locus_tag="VHM48_00270"
     CDS             complement(545..1693)
                     /locus_tag="VHM48_00270"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX2753863.1"
                     /translation="MSVLDLRSDTVTQPTAEMRRAMAAAEVGDDVWGEDPTVNALEER
                     AAELLGKEAGLFVSSGTMGNLVSLMAHVPRGGEVIAGAQSHIVRDEAAGHAVVVGASV
                     LQLRDRPDGTLDPDAVAAAFRDPSDPHEPTTALVAIENAHSHSGNQPISIADTEMIAA
                     VARERGVPLHIDGARFFNAVVALGVSAAELAAPADSVTFCLSKGLSCPAGSVVVGSSS
                     FVGRARRARKLLGGGMRQAGVLAAAGLVALRDGDAGMIARLADDHANARRLAELVAGI
                     PGVCSPGDIAQPGGNGEPPDEPLDPARVRTNFVLFRVAADRSAFLAAAEARGVRLDSY
                     PHGQIRAATNSGIAADDIDRIARVVAAALDEIGSARSTAPVPSVAGRR"
     gene            1784..3475
                     /locus_tag="VHM48_00275"
     CDS             1784..3475
                     /locus_tag="VHM48_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753864.1"
                     /translation="MTERRGDQGFSMAPQRLGETGGGPRRSRLRGIGLAAILVSAAAI
                     VTIAWIGPRLTDRPSFEISFFATPVPSVTPSPTPTVGPVLGDSLATPLPAITRPDGPL
                     PTGRIAIQTDAFRVLDLATGAVVTGPRAIYGRDALFRAPTGDGWTCICFDDGELTGAT
                     GRAMDLVEIGPTGELTRSTTIATFKMTTSTFTGQADLATDVDIFDGRHRGLLAIATHD
                     GKLWRFAVAPLDVDGRQLGASVDLGNAIGPAAEPSPNTLPTPGPQDLYLDGPHVRVAP
                     GGHVAFVWGVLQQSGTDGLPISTIHAWRVALGADGSIGEVTEAPGLLSLPLFCSTIGF
                     ASSDRLAWLCPIFDPVSGSNGGWKLGTVDLDGRAAGQTELTVGSESYFEPLFDRANGQ
                     IYVWDPGQLSIARIDAYTLVVERATFEPLVQSSSGLPPGGGSAPVDWHHGNSAILYSS
                     GLLAGAADGGRLYAVGLDPASTVDSGAQPSRGIFVIDRATLALVDRWAPATEYVAVTA
                     LPGGLVAAAGIPGVKEDGHFAPWEASLTIHDAADGRILVRFGQLGNDLPPIVVDR"
     gene            3557..3633
                     /locus_tag="VHM48_00280"
     tRNA            3557..3633
                     /locus_tag="VHM48_00280"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3591..3593,aa:Asp,seq:gtc)
     gene            3719..>4246
                     /locus_tag="VHM48_00285"
     CDS             3719..>4246
                     /locus_tag="VHM48_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753865.1"
                     /translation="MRRLPVVVVAGLVVVAAVVSALGFWTAPAPSGSPAPSTIARASS
                     SPPPRGSARPTASSASTPSASLPPVDSPLADVPIVPVAQYRTTAEKVTRAEVQAAFDG
                     NGTTWNGLELVESEAPAILAGLGLQGPGGIDRLFLVPDAATVMRDLAKNRKRLAILRA
                     DAVGPGVRALGWGGHT"
BASE COUNT          559 a         1556 c         1511 g          620 t
ORIGIN      
        1 aggcgggcgt cgggcgacag cccttcgacg tcgatcgcct cgaccgccgc gaggtgggct
       61 ttctcggcag caagctcggc gaggaccgcg tcgcgggtgc cgtcgcccag ccggtggtcc
      121 tcggtgtgga tgccgaggta cgtcccgacg agcgggttct cggcgacgat ccgccggaac
      181 cgggcctcga cgaggtcgta gaagcgctca tcgagcgggc cggcgtcggc agtcggcgca
      241 gggcgggtca ggacgacatc ctgcagggtt ccggtcacga agtgtctcct gggtcgcggg
      301 ctcgtcgcgc gcccgtcgcg catcgttcgg gctgccgcgg gcggggcggc gtgtcgcccg
      361 acgaagggcc ctgagtgtcg cccattgcgg ccgatcgcgc cagccggacg atcggccggc
      421 ggtcccgcgc tcgcgtcgcg aggggctcct cttgcgaccg acatcgacgg gtttccagct
      481 ccactgaaca gaatcgggct tcccggcgag tgagaacccc gacgagggtg ccggcggcga
      541 cgggtcagcg gcggccggcg acgctcggaa ccggtgccgt cgagcgcgcg gacccgatct
      601 catccagcgc ggcggcgacg acgcgggcga tccgatcgat gtcgtcggcg gcgatgccgc
      661 tgttcgtggc ggcccggatc tggccgtgcg gataggagtc gaggcggacg ccgcgggcct
      721 cggccgcggc gaggaacgcc gatcggtccg cggcgacacg gaacaggacg aagttggtcc
      781 ggacgcgggc cgggtcgagc ggctcgtcgg gcggttcgcc attgcccccc ggctgggcga
      841 tgtcgcccgg cgagcagacc cccggaatgc ccgccaccag ctccgccagg cgacgcgcgt
      901 tggcgtggtc gtcggcgagt cgcgcgatca tccccgcgtc gccgtcccgg agcgcgacga
      961 gaccggccgc cgcgaggacg cccgcctggc gcatcccgcc accgagcagc ttgcgggcgc
     1021 ggcgggcccg gccgacgaag gacgacgagc cgacgacgac cgagccggcc gggcaggaca
     1081 gccccttcga caggcagaac gtgaccgagt ccgcgggcgc agcgagctcc gccgcgctga
     1141 ccccgagggc gacgaccgcg ttgaagaacc gcgccccatc gatatggagc ggcacgcccc
     1201 gctcgcgggc gaccgccgcg atcatttccg tgtccgcgat cgagatcggc tggttgcccg
     1261 agtgactgtg ggcgttctcg atcgcgacga gcgcagtcgt cggttcgtgc gggtccgacg
     1321 ggtcgcggaa ggcggcggcc accgcatcgg gatccagggt tccatcgggc cggtcgcgca
     1381 gttggagcac gctcgccccg acgaccacgg cgtggccggc ggcctcgtcg cggacgatgt
     1441 ggctctgggc gccggcgatg acctcgccac cgcgcggcac gtgggccatc agcgagacga
     1501 ggttgcccat cgtccccgac gagacgaaga gccccgcctc cttgcccagc agctcggccg
     1561 cccgttcctc gagggcgttg acggtcggat cttcgcccca gacgtcgtcg ccgacctcgg
     1621 ccgcggccat cgcccggcgc atctcggcgg tcggctgggt gacggtgtcg gagcggaggt
     1681 cgagcacgga catgggccgc gagtataggc gcgagcatgt cggcttcggc acgccaggaa
     1741 cacgccggga acccgacgaa ggcgcgcgtt gtagggtgag atggtgacgg agcggcgcgg
     1801 cgaccagggc ttttcgatgg cgccgcaacg gctcggggag accggcggtg gaccgcgccg
     1861 atcgcggttg cgcggcatcg ggctcgcggc gatcctcgta tcggccgccg caatcgtcac
     1921 gatcgcctgg atcgggccgc ggttgacgga tcgaccgagc ttcgagatct cgttcttcgc
     1981 gacgcccgtg ccatcggtca cgccgtcgcc cacgcccacg gtcggaccgg tccttggcga
     2041 ttccctcgcc accccgttgc ccgcgatcac gcggcccgac ggcccgctcc cgaccgggcg
     2101 gatcgcgatc cagaccgacg cattccgcgt cctcgatctc gcaaccggcg cggtcgtgac
     2161 cggtccccgc gccatctacg gcagggacgc cctcttccgg gcgccgaccg gtgatggctg
     2221 gacgtgcatc tgcttcgacg acggcgagct cacgggtgcc acgggccggg cgatggacct
     2281 ggtcgagatc gggccgaccg gggaattgac ccgttccacc accatcgcga ccttcaagat
     2341 gacgacgtcg accttcactg gccaggccga tctggcgacc gacgtcgaca tcttcgacgg
     2401 ccgccaccgg ggcctcctcg caatcgcgac ccacgacggc aagctgtggc ggttcgcagt
     2461 cgcaccgctc gatgtcgacg gtcggcaact cggagcatcg gtcgacctgg gcaacgcgat
     2521 cgggccggcc gccgagccgt cgccgaacac gctgccgacg cccggcccgc aggatctcta
     2581 cctcgatggt ccccacgtcc gggtcgcccc gggcggccac gtcgccttcg tctggggtgt
     2641 tctccagcaa tccgggaccg atggacttcc gatcagcacg atccatgcct ggcgcgtggc
     2701 gctcggcgct gacggttcga tcggcgaggt gaccgaggcg cccgggttgc tcagcctgcc
     2761 gctcttctgc agcaccatcg ggttcgcctc ctccgaccgc ctggcgtggc tctgtccgat
     2821 cttcgatccg gtgtccggct ccaacggggg ctggaagctc gggaccgtgg atctcgacgg
     2881 tcgggccgcc ggccagaccg agctcaccgt cggaagcgag tcgtacttcg agccgctctt
     2941 cgaccgggcg aatggccaga tctacgtgtg ggatccgggc cagctgtcga tcgcccggat
     3001 cgacgcctac acgctggtgg tcgagagggc caccttcgaa ccgctcgtcc aatcgtcgag
     3061 cggcctcccg cccggtggcg ggtccgcgcc tgtcgactgg caccacggta actcggccat
     3121 cctgtacagc tccggcctgc tggcgggcgc ggccgatggc ggacgcctgt atgccgtcgg
     3181 actggatccg gcttcgacgg tcgactccgg cgcccagccg agccgcggga tcttcgtgat
     3241 cgatcgggcc accctggccc tggtcgatcg gtgggcgccg gcgaccgagt atgtcgcagt
     3301 gaccgcgctg cccggtggcc tcgttgccgc cgccggaata cccggggtca aggaggacgg
     3361 ccacttcgcc ccgtgggagg catcgctgac cattcacgat gcggcggacg gtcgaatcct
     3421 cgtccggttc ggccagctgg gcaacgacct cccgccgatc gtcgtcgatc gctgagcccg
     3481 gacggccgag ccgcccgggc tcgcacgaca agcgacaacg gtggattcct ctgatatcct
     3541 ccgacctcgc cgcactggac ccgtggtgta gcggcccaac atgccagcct gtcacgctgg
     3601 agatcgccgg ttcgaatccg gtcgggtccg ccattcttcg catgcaccca cgccccgtcc
     3661 gcccgcccgg acggggcgtc tctctgcccg gtaggcgctt cgtggcatca tcgggccggt
     3721 gagacgtctc cccgtcgtcg tcgtggccgg cctcgtcgtc gtcgccgccg tggtgtccgc
     3781 gctcgggttc tggacggcac ctgcgccatc cggctcgccc gctccgagca cgatcgctcg
     3841 cgcgtcgtcg tcgccgcctc cgcgcgggtc tgcgcggccg accgcctcca gcgcctcgac
     3901 cccgtcggcc agcctgccgc cggtcgactc gcccctcgcc gacgtcccga tcgtgcccgt
     3961 cgcccagtac cggacgaccg cggagaaggt cacccgcgcc gaagtccagg cggcgttcga
     4021 cggcaatggc acgacctgga acggcctcga gctcgtcgag agcgaggccc ccgcgatcct
     4081 ggccggcctc ggcctccagg gacccggcgg gatcgaccgt ctcttcctcg ttcccgacgc
     4141 cgcgaccgtg atgcgggatc tcgcgaagaa ccgcaagcgc ctcgcgatcc tccgggccga
     4201 cgccgtcgga ccgggcgtcc gggccctcgg ctggggcggc cacacc
//
LOCUS       DATAIW010000010        11562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_290, whole genome shotgun sequence.
ACCESSION   DATAIW010000010 DATAIW010000000
VERSION     DATAIW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /gene="glmS"
                     /locus_tag="VHM48_00290"
     CDS             <1..1773
                     /gene="glmS"
                     /locus_tag="VHM48_00290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2753866.1"
                     /translation="LQRLEYRGYDSAGIALVDEDGDLFVEKKAGKLANLQTAIADRTP
                     HAAIGLAHTRWATHGRPNDLNAHPHQDCTGDITVIHNGIIENFSELRDGLEARGHTLT
                     SETDTEAIAHLVVEAYEGDLADAVRATLRQLDGAYALVVMHRREISRLVGARQDVPLV
                     VGRHGEESFIASDVAAILAHTNRIIFLEEGDVADVRPTGVVITDVAGVVQERSETVVD
                     WTPEAAEKGGFDHFMLKEIHEQPEALRQCIAGRVTRDGRIRVAEIDGLAEQIRAVTRV
                     ELVACGSAYYAALVGAAAIEEWTGIPARATVGSEFRYSPPPLDARTLVIAVTQSGETA
                     DTIAPTRHARERGCPIVAVTNTVGSAITREADAVVFLHAGPEIAVAASKTFTTQVTTL
                     VMVAAAIARIRGALTEDAERALADALHALPSAAQRTIEANEPGAAAIARRYVNSRGFM
                     FVGRGYAMPTALEGALKLKEVSYVHAEGYAAGELKHGPISLLDAEYPLVAVATRSRVY
                     DKLISNVMEGRARDARVLAVATEGDEQIGRYADDVWYVPDTHEVLSPVIGIIPLQLFA
                     YHVAVARGTDVDQPRNLAKSVTVE"
     gene            1787..2428
                     /gene="acpS"
                     /locus_tag="VHM48_00295"
     CDS             1787..2428
                     /gene="acpS"
                     /locus_tag="VHM48_00295"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX2753867.1"
                     /translation="MSRQPPRPPDTGPPEVAGGAPRPHAPDGGRAPRGQPPDGTSELG
                     IDIIKVERIRASIARFGERFAKRVLTTNEQRYVRMRPETFAGRWAAKEAVSKVLGLGV
                     RGIGWRDIEVERLPTGQPAIRLHGRAAARAEQLGMDRIAVSITHESDYAVAIAFGVRT
                     TAGRYVFPLDIDERLDDRERRLLARMERLRELHAANAALEEGASMAAIEGVET"
     gene            2425..3543
                     /locus_tag="VHM48_00300"
     CDS             2425..3543
                     /locus_tag="VHM48_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2753868.1"
                     /translation="MTGRGRLGFTRHDARPDPAPESIAPPVDGAEILDDAIASALLPV
                     RDKRGHKGAFGKLLVIAGSLDYAGAALLVCRAAGRAGAGLVTLAVPESLQPLFAAKVI
                     EATTMGLPEDDVEEIDPEASMARILDHDHDAIVIGPGLRPGLATAELVRMLLTTAGDA
                     GAAPILLDAEALRSLATLERWWEGDRRPSVLTPHAGEFARLRAGSGRDPDDDGDLASD
                     DAARATAAGDAAATWRQVVVLKGAKTVIAAPDGRVSVAPFENPALASGGTGDVLAGTI
                     GSLLAQHVPPYDAARLGVYLHGLAGDTVRERLGDAGLLASDLPDPIALARKRLAGVAE
                     RRKAGGRLGFGVRPPGSGGSRQPDAPDVADPEPSGGSA"
     gene            3546..4865
                     /gene="alr"
                     /locus_tag="VHM48_00305"
     CDS             3546..4865
                     /gene="alr"
                     /locus_tag="VHM48_00305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2753869.1"
                     /translation="MTSQPTRPTASIEERLARAGLPPLGRTAWIEVDTAALAANLQVL
                     RAMAGDVAVLPVVKADAYGHGMVPVARTLAAAGADGLCVATLDEAIILDDAGVAVPLL
                     VLFPIPSSGVVEAARRGIGLAGGAASALDEMLSAAVAAGMADRLSIELEIETGLGRGG
                     VLPEAAVDAARRILASGATVTGVWSHLQEAEVADLTTKQIARFDEALARLAAAGIIIP
                     RRHLAASAGILLGTVPRYDAVRPGLAIYGLIPDELAAAGIGLDDLLLAARELRPILSL
                     HARPVRVTDLPTGHGVSYGPTWRAPRPSRIATLPLGYGDGWPRSLSNRAEVLVRGVRV
                     PMVGNVAMDAAMIDVSGVPGRPVDIDDEVVLIGRQGDAEIRVADVARWRTTNSWEVVT
                     AMSARLTRVYDAAAGLTGSRPLAQLENPWPGSSSGTATSATSRSTRS"
     gene            4805..5401
                     /locus_tag="VHM48_00310"
     CDS             4805..5401
                     /locus_tag="VHM48_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX2753870.1"
                     /translation="MARIELWNGDICDLEVDAIVNAANLSLWMSTGVSGEIKRAGGDA
                     IEFAAVRQAPVSLGEAIVTPAGRLAAKVIIHAVSLDRDRRTSGEAIDRAVRSAMARAR
                     ELRVASMAFPAMGTGVGGFPLDEAARVTVRAVRTELARSPLIDHVTFAMRGATAYQAF
                     ELALAEGGIDDSRPAAAEPAAADPTPAEGHQVDGAVHA"
     gene            5398..5688
                     /locus_tag="VHM48_00315"
     CDS             5398..5688
                     /locus_tag="VHM48_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753871.1"
                     /translation="MSMPYERSEEQRDELVERVAREIQLRGLTGPAVHFLEASRPYRP
                     LGANAMLFFDPVLRGLFGGDDASASEILADDTGIELLIARLEEIDEEITWDA"
     gene            5970..7604
                     /locus_tag="VHM48_00320"
     CDS             5970..7604
                     /locus_tag="VHM48_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753872.1"
                     /translation="MSSEPLAFLALDLGAATSSAALIGRLARRWRLIGSLSMPSSSSV
                     DALIVELIRRLVAADPALAVSIGLADDDGTIDAVRLGAELPRLVARSAPARTMLVCAV
                     SDRALAPLAAAARRTGWRTLETALDTVEPLELTSRLLDPAVDVLLVGAGDPPGADERR
                     RLDELAAIVAGAVARRPDLSVVLAGAMADHLQRIESSTGDRSGELLLAPAAAAGHPAG
                     SALRQLLDEIRGGPDDPRRAAGRAVGALADVLDRRVELIEIGYDGGLRATASPGIGGN
                     DAVTTVSIVADACLVPPESTDETVDRVLAWSTMSLDRHRLRDRLRELRLWPWSGIAGE
                     GARLRLAAARAAMTILVDATPEQSALPAPDLVVVSGGAFAVAPGPAVALAVADVLRRP
                     GAVALAQDHARLLGTIGVIPDATERRQALLDLVDDLLTPLGSVVIPGGIRAGASAGRM
                     TVHAGTGSSDTDLVPGGLEVVDLAPGEVATAEFQFRDAVRLGTRGRRFAVDVSGGLGG
                     LLVDLRDVPLRLPERMDRRRELLEAWQESLWLTRDR"
     gene            7601..8689
                     /locus_tag="VHM48_00325"
     CDS             7601..8689
                     /locus_tag="VHM48_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753873.1"
                     /translation="MTSTRVRPARPGAAATSPSLATGDLRGLDLVACRPVLVGPTEIL
                     FRLAPGDRALVAPGDAIVAGTAIAERLRDARAVELAPSATSDGARAGDRWMTAGQTGV
                     RLRRGSGAEAGELLYEHGGHWVLAAGEHAEAVDCPLAGVVRDVRPGIGIRVRAQGRAL
                     LGVEALGDPTFGLLSLGSERGDQRHGRLRSTSLDVGLAGTILVIGSRVDAEALTRARA
                     MGVRGVIVSGLAGKERRDFLASESRQRAALHRLPPFAVLVLDGAIRRPIAGPVAAVLE
                     ALSGKTVAITIDPPALVFDEPALSVAAPPADHVRVRAGEHAGEEGRWAGLAGVRRFAG
                     GSFLEAGWVVLGDDPPVSIPIADLERFA"
     gene            8726..9199
                     /gene="tsaE"
                     /locus_tag="VHM48_00330"
     CDS             8726..9199
                     /gene="tsaE"
                     /locus_tag="VHM48_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX2753874.1"
                     /translation="MPAAAELRVRVRTASAGETIALGERLGRASAPGDLVCLWGDLGA
                     GKTQLAKGIARGLGIADTVNSPTFILMNEYGGRLPLFHVDLYRLANAADALAGGVVDD
                     RQIDGVTVVEWPDRMGDVLPAGRLDVRIEGSGDEVREIEVATTDQRYARLVEAAR"
     gene            9196..9915
                     /gene="tsaB"
                     /locus_tag="VHM48_00335"
     CDS             9196..9915
                     /gene="tsaB"
                     /locus_tag="VHM48_00335"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX2753875.1"
                     /translation="MNGAGEDGRRAGGGVADGRRSWLLAIDTATSRVVVAAAALDGTP
                     LGVSTWEAGRTHGAQLLPAIGRLTGEANLRRARIRGVIVGTGPGAFTGLRVGIATAKA
                     LAHELAVPIVGVSTGAALLEASGAGERGVLLLPAGPNDRLVVRSGTAAVLLPGGSELD
                     VDPGDILVAVDLDDRAPADAVARGEAARAGFPAAMVRLGAARLAAGDVDDLARLVPDY
                     VSLPRGVERESGEVAWSRDRP"
     gene            9894..10499
                     /gene="rimI"
                     /locus_tag="VHM48_00340"
     CDS             9894..10499
                     /gene="rimI"
                     /locus_tag="VHM48_00340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX2753876.1"
                     /translation="MVARPPVKLTIEAMRLEDLSEVQRIEQASFTTPWPENAYRSELM
                     TNRLASYLVARVGGRIVAYGGMWLMVDEAHITTFAVHPAWRRQRIGERLLLAFLDLAR
                     DRHAREATLEVRLSNLAARRLYEKYGFRPVGLRPRYYSDNNEDALIMTTESFGDPALE
                     ARLVRLRAAIDDGPAPTGPFEPDPDPDLRQRRDEPPGEADR"
     gene            10496..>11562
                     /gene="tsaD"
                     /locus_tag="VHM48_00345"
     CDS             10496..>11562
                     /gene="tsaD"
                     /locus_tag="VHM48_00345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2753877.1"
                     /translation="MTGGAPSPGSLLLSVESSCDETGIAVIDGGRRILSNVVASQTAL
                     HAASGGIVPEVAARAHLRWIVPVLDQALVDAGVTVDDVDAVAVTHGPGLAGSLLVGIN
                     FAKTIAWVHDKPLVGVNHLEGHLYAAWLTDPGEDEREEPRFPLVALIVSGGHTFLVEM
                     RDHLTYRLLGQTVDDAAGEAFDKVGRLLGLPYPGGPAVSRAAERAVRHDAVFPRAWLG
                     QTYDFSFSGLKTAARRTIDEARAGEGLPTGKAAASEPDARLSEAAVAELSWGFQDAVV
                     DVLVTKAIRAAEETGARSIVLGGGVASNAALRARLAEEAESRGLPLIVPRPALCTDNG
                     AMIGAAGARRFAAGERADLSL"
BASE COUNT         1473 a         4308 c         4182 g         1599 t
ORIGIN      
        1 ctccagcggc tcgagtatcg cggctacgac tcggccggca tcgccctcgt cgacgaggac
       61 ggcgacctgt tcgtcgagaa gaaggccggc aagctcgcga acctccagac cgccatcgcc
      121 gaccggacgc cccatgccgc gatcggcctg gcccacaccc gctgggcgac gcacggccgg
      181 ccgaacgacc tcaatgccca tccccaccag gactgcaccg gcgacatcac ggtcatccac
      241 aacgggatca tcgagaactt cagcgagctc cgtgacggcc tcgaggcgcg cggccacacc
      301 ctgacctccg agacggacac cgaggcgatt gcccacctcg tcgtggaggc ctacgagggc
      361 gacctggccg atgccgtccg ggcgaccctc cgccagctcg atggcgccta cgccctcgtt
      421 gtcatgcacc gccgcgagat cagtcggctg gtcggagccc gccaggacgt gcccctcgtc
      481 gtcgggcggc acggcgagga gagcttcatc gcgagcgacg tcgcggcgat cctcgcccac
      541 acgaaccgga tcatcttcct cgaggagggc gacgtcgccg atgtccgccc gaccggggtc
      601 gtcatcaccg acgtcgcggg cgtggtccag gaacggtccg agacggtcgt cgactggacg
      661 ccggaagcgg ccgagaaggg cggcttcgac catttcatgc tcaaggagat ccacgagcag
      721 cccgaggccc tccgccaatg catcgccggc cgggtgaccc gcgacggccg catccgggtc
      781 gccgagatcg acggcctggc cgagcagatc cgggcggtca cccgggtcga gctcgtcgcc
      841 tgcggcagcg cctactacgc ggcgctcgtc ggcgcggcgg cgatcgagga gtggaccggg
      901 atccccgccc gggcgaccgt cggctccgag ttccgctaca gcccaccgcc gctcgacgcg
      961 agaacgctcg tcatcgcggt cacccagtcc ggcgagaccg ccgacacgat cgccccgacc
     1021 cgtcatgccc gcgagcgcgg ctgcccgatt gtcgcggtga cgaacaccgt cggctcggcg
     1081 atcacccgcg aggccgatgc ggtcgtcttc ctccatgccg ggccggagat cgcggtcgcg
     1141 gcgtcgaaga ccttcacgac ccaggtcacg acgctcgtca tggtcgccgc ggcgatcgcc
     1201 cgcatccggg gcgcgctcac cgaagacgcg gagcgggcgc tcgcggacgc gctccacgcc
     1261 ctgccgtcgg cggcccagcg aacgatcgaa gcgaacgagc cgggcgccgc cgcgatcgct
     1321 cgtcgctacg tgaactcacg cggcttcatg ttcgtcggcc gaggttacgc gatgccgacc
     1381 gcgctcgagg gcgccctcaa gctcaaggag gtcagctatg tccatgccga gggctacgcg
     1441 gcaggagagc tcaagcacgg cccgatctcg ctgctcgacg cggagtaccc actcgtcgcg
     1501 gtggcgaccc gctcgcgggt ctacgacaag ctgatcagca atgtcatgga gggccgcgcc
     1561 cgcgacgccc gcgtcctcgc ggtcgcgacc gagggcgacg agcagatcgg gcgctacgcc
     1621 gacgacgtct ggtacgtccc cgacacccac gaggtgctga gcccggtcat cgggatcatc
     1681 ccgctccagc tgttcgccta ccacgtggcc gtcgcccgcg gcaccgatgt cgaccagccg
     1741 cggaacctcg ccaagtcggt cacggtcgag taggtcgccg aacgtcgtga gccggcaacc
     1801 tccccgtccg ccagacacgg ggcccccgga ggtcgcaggg ggagctccgc gaccccatgc
     1861 gcccgacggc ggccgagctc cgcgaggcca accgcccgac ggcacctcgg agctcgggat
     1921 cgacatcatc aaggtcgagc ggatccgggc gtcgatcgcg cggttcgggg agcgcttcgc
     1981 caagcgggtc ctgacgacca acgagcaacg ctacgtccgg atgcggcccg agacgttcgc
     2041 cggccgctgg gcggcgaagg aagccgtgtc gaaggtcctc ggcctcgggg tccgggggat
     2101 cggctggcgt gacatcgagg tcgagcggct gccgaccggc caaccggcga tccgcctgca
     2161 cggacgggcg gcggcgcggg ccgagcagct cgggatggac cggatcgcgg tctcgatcac
     2221 gcatgaatcg gactacgccg tggcgatcgc gttcggtgtc cggaccaccg ccgggcgcta
     2281 cgtcttcccg ctcgacatcg acgagcgcct cgacgatcgt gagcggcgcc tgctcgccag
     2341 gatggagcgc ctgcgcgagc ttcacgcggc caatgcggca ctggaggaag gggcgtccat
     2401 ggccgcaatc gaaggcgtcg agacgtgacc gggcgcggcc ggctcgggtt cactcgccac
     2461 gacgcccggc cggatcccgc tccggagtcg atcgcgccgc ccgtcgacgg cgccgagatc
     2521 ctcgatgacg cgatcgcgtc ggcgctcctg ccggtccgcg acaagcgggg gcacaagggt
     2581 gcgttcggga agctgctcgt gatcgccggc tcgctcgact acgccggcgc cgccctcctc
     2641 gtctgtcgcg ccgcgggacg ggccggcgcc gggctcgtca ccctggcggt cccggaatcg
     2701 ctgcagccgc tcttcgcggc caaggtcatc gaggccacga cgatgggcct cccggaggac
     2761 gacgtcgagg agatcgaccc ggaggcttcg atggcccgga tcctcgacca cgaccacgat
     2821 gccatcgtga tcggacccgg gctccgaccc ggcctcgcga ccgccgagct cgtccggatg
     2881 ctcctcacca cggccggtga cgccggtgcc gcgccgatcc tcctcgacgc cgaggcgctg
     2941 cgctccctcg ccaccctcga gcgctggtgg gagggtgatc gccggccgag cgtcctcacc
     3001 ccgcacgccg gggagttcgc tcgtctccgg gccggcagcg gccgcgatcc cgacgacgac
     3061 ggcgacctcg cctcggacga tgctgcgcga gcgaccgcgg ccggcgatgc cgcggcgacc
     3121 tggcgccagg tcgtcgtgct gaagggggcg aagaccgtga tcgccgcccc ggacggacgg
     3181 gtctccgttg ccccgttcga gaacccggcc ctggcgtccg ggggcaccgg cgacgtcctg
     3241 gcgggcacga tcggctcgct cctggcccag catgtcccgc catacgatgc ggcgcggctc
     3301 ggggtctacc tccacggcct tgcaggggac accgttcgcg agcggctcgg tgacgcgggc
     3361 cttctcgcgt cggacctgcc ggacccgatc gccctcgccc gcaagcggct cgccggcgtc
     3421 gccgagcgac ggaaggccgg tgggcgcctc ggcttcggcg tgcggccacc cggatccggc
     3481 ggctcgcgcc agcccgacgc cccggacgtc gccgacccgg agccgagcgg cggctccgcc
     3541 tgaccgtgac cagccagccg acccgtccga ccgcgtcgat cgaggagcgg ctggcccggg
     3601 ccgggcttcc gccgctcggg cggacggcct ggatcgaggt cgacacggcg gccctcgccg
     3661 cgaacctcca ggtgctgcgg gcgatggccg gcgacgttgc cgtcctgccg gtggtcaagg
     3721 ccgatgccta cggccacggc atggtgccgg tcgcccggac gcttgccgcc gccggcgccg
     3781 acggcctgtg cgtcgcgacc ctcgacgagg cgatcatcct cgatgacgcg ggggtcgcgg
     3841 ttccgcttct cgtgctcttt ccgatcccgt cgagcggcgt cgtggaggcc gcgcggcggg
     3901 ggatcgggct cgccggcgga gcggcgagcg ccctcgacga aatgctttcg gcagcggttg
     3961 cggcgggcat ggcggaccgc ctgtcgatcg agctcgagat cgagaccggg ctcgggcgcg
     4021 gcggcgtcct gccggaggcc gcggtcgacg ccgcccggcg gatccttgcc agcggggcca
     4081 cggtcaccgg cgtctggtcg cacctgcagg aggcggaggt cgccgacctg acgacgaagc
     4141 agatcgcccg gttcgatgag gcgctcgccc gcctggctgc cgccgggatc atcatcccgc
     4201 ggcgtcacct cgccgcgagc gccgggatcc tgctcggcac ggttccccgc tacgacgctg
     4261 tccggcccgg tctcgcgatc tacgggttga tccccgacga gctcgccgcg gccggcatcg
     4321 ggctcgacga cctcctgctc gcggcccgcg agctgagacc catcctctcg ctccatgccc
     4381 ggccggtccg cgtcacggac ctgccgaccg gacacggcgt ctcctacggc ccgacgtggc
     4441 gggcgccccg ccccagccgg atcgcgaccc tgccgctcgg ctatggcgac ggctggccgc
     4501 ggtcgctctc gaaccgggcg gaagtgctcg tccgcggcgt ccgggtcccg atggtcggca
     4561 acgtcgcgat ggacgccgcg atgatcgatg tcagcggtgt tccgggtcgc cctgtcgaca
     4621 tcgacgacga ggtcgtcctg atcggccgcc agggcgacgc cgagatccgc gtggccgatg
     4681 tggcgcgatg gcgcaccacg aactcctggg aggtcgtgac cgcgatgtct gcccgcctga
     4741 cccgggtgta cgatgcggcg gccggtctga ccggatcgcg cccgcttgcc cagctggaga
     4801 acccttggcc cggatcgagc tctggaacgg cgacatctgc gacctcgagg tcgacgcgat
     4861 cgtgaacgcc gccaacctgt ctctgtggat gtcaaccggg gtttccggcg agatcaagcg
     4921 cgccggcggt gacgcgatcg aattcgccgc cgtccgccag gcgcccgttt cccttggcga
     4981 ggcgatcgtg accccggccg gccggcttgc cgccaaggtg atcatccatg ccgtgtcgct
     5041 cgatcgcgac cgccggacga gcggcgaggc catcgaccgc gcggttcgca gcgcgatggc
     5101 tcgggcccgt gagctgcgcg tcgcgagcat ggccttcccg gcgatgggta ccggcgtcgg
     5161 gggctttccg ctcgatgagg cggccagggt gacggtccgc gccgttcgca ccgaactggc
     5221 gcgatcgccg ctcatcgacc acgtgacctt cgcgatgcgg ggtgccacgg cataccaggc
     5281 gttcgagctc gcgctcgcgg aaggcggcat cgacgactcg agaccggcgg ctgccgaacc
     5341 ggcggcggcg gacccgacgc cggccgaagg ccaccaggtc gacggagcgg tgcacgcatg
     5401 agcatgccct acgagcggag cgaggagcag cgcgacgagc tggtcgagcg cgttgcccgc
     5461 gagatccagc tccgcgggct gaccggtccc gccgtccatt tcctcgaggc cagccggccg
     5521 tatcgacccc tcggggcgaa cgcgatgttg ttcttcgacc cggtcctgcg cggcctcttc
     5581 ggcggcgatg acgcgtcggc gtcggagatc ctcgccgacg acaccgggat cgagctgctc
     5641 atcgcccggc tggaagaaat cgacgaagag atcacctggg acgcatagtc ggctgaccgg
     5701 cgcgccctgt ccgcggcaga gccccggccc cgcaccggag gtccctcgcc gtgtgcgcac
     5761 tgtgcgggca ctgcgacgaa cgagggaacg cccggccgtt gttctgcccg cccgttgcgc
     5821 aatccgacca aggagcccgt gatctcgcgc gatcgacgat gcctccttcg ccgcagcgct
     5881 caccgggcat gcacatagcg aacggcgcgc ctgtgcagcg aaaggggccg agctcctgcg
     5941 gggccatcgg cccgaggtag catggtcggg tgagctccga acccctcgcc ttcctcgccc
     6001 tcgacctcgg ggctgcgacc tcgtccgcgg cgttgatcgg ccggctcgcc cgtcgctggc
     6061 ggctgatcgg gtcgctctcg atgccgtcgt cgagcagcgt cgatgccctc atcgtcgagc
     6121 tgatccgacg actcgttgcc gccgatccgg cactcgccgt gtcgatcggc ctggcggacg
     6181 atgacggcac gatcgacgcc gtccgcctgg gggccgagct cccgcgcctc gtggcccgca
     6241 gcgctccggc ccggacgatg ctcgtatgcg cggtctccga ccgggccctc gccccgctcg
     6301 ccgcggccgc ccgccggacc ggctggcgga cgctcgagac ggccctcgat actgtcgagc
     6361 ccctcgagct gacctcgcgc ctgctcgatc cggcggtcga cgtcctgctc gtcggagcgg
     6421 gcgatcctcc aggcgcggac gagcggcgac gactcgacga gctggcggcg atcgtcgccg
     6481 gggctgttgc tcgccggccg gacctgtccg tcgtcctcgc cggggcgatg gccgatcacc
     6541 tgcagcgcat cgagtcgtcc accggcgatc gcagcggcga gctcctgctc gctccggccg
     6601 ccgcggccgg ccatccagcg ggatcggcgc tgcgccagct cctcgacgag atccgcggtg
     6661 gtccggatga tccacgccgc gcggccggcc gggccgtggg cgccctcgcc gacgtcctcg
     6721 accggcgggt cgagctgatc gagatcggct acgacggcgg cctccgggcg acagccagcc
     6781 ccggcatcgg gggcaatgac gcggtcacga ccgtatcgat cgtggccgat gcgtgtctcg
     6841 tcccgcccga gtcgaccgac gaaacggtcg atcgcgtcct cgcctggtcg acgatgtcac
     6901 tcgatcgcca tcggttgcgc gaccgtctcc gcgagctgcg gctctggccg tggtccggga
     6961 tcgccggaga aggcgctcga ctccggctgg ccgcggcccg cgcggcgatg acgatcctcg
     7021 tcgatgcgac gcccgagcaa tcggccctgc cggcaccgga cctcgtcgtc gtctccgggg
     7081 gtgcgtttgc ggtcgctccc ggcccggcgg tcgccctcgc cgtcgcggac gtgctccgtc
     7141 gacccggcgc ggtcgccctc gcccaggacc atgcccggtt gctgggcacg atcggggtca
     7201 tccccgacgc gaccgagcga cgccaggccc tgctcgatct cgtcgacgac ctgctcacgc
     7261 ctctcggcag cgtcgtgatc ccgggcggca tccgggcggg tgccagtgcc ggccggatga
     7321 ccgtccacgc cggcaccgga tcgagcgaca ccgacctcgt gccgggcggg ctggaggtcg
     7381 tcgacctggc cccgggcgag gtcgcgacgg ccgagttcca gttccgcgat gccgtccgcc
     7441 tcgggacccg cggccggcgg ttcgcggtcg atgtctccgg cgggttgggc gggctgctcg
     7501 tcgacctccg tgacgttccg ctccgcctgc ccgaacggat ggatcgccgc cgcgagctgc
     7561 tcgaggcgtg gcaggaatcg ctgtggctga cgcgcgaccg atgacgagca cgcgcgtccg
     7621 gcccgcgcgg cccggagccg cagccacctc gccgtcgctt gcgaccggcg acctgcgggg
     7681 cctggacctc gttgcctgtc gcccggtcct ggtcggtccg accgagatcc tcttccgcct
     7741 cgctcccggt gaccgggcgc tcgttgcccc gggcgacgcg atcgtggccg ggaccgcgat
     7801 cgccgaacgg ctccgggacg cccgggcggt cgagctcgcc ccttcggcga cgagcgacgg
     7861 cgcccgggcc ggcgaccgct ggatgacggc aggccagacc ggggtccggc tccggcgcgg
     7921 ttccggtgcc gaggcgggcg agctcctcta cgagcacggc ggccactggg tgctcgccgc
     7981 cggcgagcac gccgaggcgg tcgactgccc cctcgccggg gtcgtccgcg acgtccggcc
     8041 gggcatcggt atccgcgtcc gggcccaggg ccgggccctg ctcggcgtcg aggccctggg
     8101 tgatccgacg ttcggcctcc tgtcgcttgg cagcgagcgg ggcgatcagc gccacggccg
     8161 gctgcgctcg acatcgctcg acgtcgggct ggccggaacg atcctcgtca tcggctcgcg
     8221 ggtggacgcc gaggcgctga cgcgagcccg tgcgatgggt gtccgaggcg tgatcgtgag
     8281 cggcctcgcg ggcaaggaac ggcgtgactt cctcgcgtcg gagtcgcgcc agcgggccgc
     8341 cctccaccgg ctcccgccgt tcgcggtcct cgtcctcgac ggcgcgatcc gccgcccgat
     8401 cgcggggccg gtcgcggccg tcctggaagc cctgtcgggc aagaccgtcg cgatcaccat
     8461 cgatccgccg gcgctggtct tcgacgagcc ggcgctctcg gtcgccgcgc cgccggccga
     8521 tcacgtccgc gtccgcgccg gggagcacgc cggcgaggag ggccggtggg ccggtctggc
     8581 cggcgtccgg cggttcgccg gcggctcctt cctcgaggcc ggttgggtcg tgctcggtga
     8641 cgatccgccc gtctcgatcc cgatcgcaga tctcgagcgg ttcgcctgac gatggcgatg
     8701 ccggctcgcc ggcgggacga ctgacatgcc cgccgccgcc gaactccggg tccgcgtccg
     8761 gacggcgtct gccggggaga cgatcgccct cggcgagcgc ctcggtcggg cgtcggcgcc
     8821 cggcgatctc gtctgcctgt ggggcgatct cggggcgggc aagacccagc tcgccaaggg
     8881 gatcgcccgc gggctcggga tcgccgacac ggtcaattcg ccgaccttca tcctcatgaa
     8941 cgagtacggc gggcgcctgc cgctcttcca cgtcgacctc taccgcctgg cgaacgcggc
     9001 cgacgccctc gccggtggcg tggtcgacga ccgccagatc gacggcgtca cggtcgtcga
     9061 gtggccggac cggatgggcg acgtcctgcc ggccggccgc ctcgacgtcc ggatcgaggg
     9121 cagcggcgac gaggtccgcg agatcgaggt ggccacgacc gatcagcgct acgcccgact
     9181 cgtcgaggcg gcccgatgaa cggggcaggg gaggatggcc gccgcgcggg tggcggggtc
     9241 gccgacggcc ggcggtcgtg gctgctggcg atcgacacgg ccacgagccg ggtcgtcgtc
     9301 gcggcggcgg cgctcgacgg gacgccgctc ggcgtctcga cctgggaggc gggtcggacc
     9361 cacggcgcgc agctcctgcc ggcgatcggg cggctgaccg gcgaagcgaa cctgcgtcgc
     9421 gcgcggatcc gcggcgtgat cgtcgggacg ggacccggcg cgttcaccgg gttgcgggtc
     9481 gggatcgcga ccgccaaggc cctcgcccac gagctcgccg tcccgatcgt cggcgtgtcg
     9541 accggcgcgg ccctcctcga ggccagtgga gccggcgagc gcggagtgct cctgctgccg
     9601 gccggcccga acgatcgcct ggtcgtccgg tccgggacgg ccgccgtgct ccttccgggc
     9661 ggctccgagc tcgacgtcga cccgggcgac atcctcgtcg ccgtcgacct cgacgatcgg
     9721 gcgccggccg acgccgtggc ccgcggtgag gcggcccggg ccggcttccc ggcggccatg
     9781 gtccggctcg gtgccgcgcg gctcgcggcc ggcgatgtcg acgaccttgc ccggctcgtt
     9841 cccgactatg tcagcctgcc gcgcggggtg gaacgcgaga gcggggaggt cgcatggtcg
     9901 cgcgaccgcc cgtgaagctc acgatcgagg cgatgcggct cgaggacctc agcgaggtcc
     9961 agcggatcga gcaggccagc ttcacgacgc cgtggcccga gaacgcctat cgcagcgagc
    10021 tgatgaccaa ccgcctggcg tcatatctcg tcgctcgcgt cggcggccgg atcgtcgcct
    10081 acggtggaat gtggctgatg gtcgacgagg cccacatcac gaccttcgcc gtccacccgg
    10141 cgtggcgccg ccagcggatc ggggagcggt tgctcctcgc gttcctcgat ctcgcccgcg
    10201 atcgccacgc ccgggaggcc accctggagg tccggctgtc gaacctggcc gcccggcggc
    10261 tctacgagaa gtacggcttc cgcccggtcg gcctgcggcc gcgctactac agcgacaaca
    10321 acgaggacgc cctgatcatg acgaccgagt cgttcggcga tccggctctc gaggcgcggc
    10381 tcgtccggct gcgggccgcc atcgatgacg gcccggcacc gacgggaccg ttcgagccgg
    10441 atcccgaccc ggacctgcgc cagcgtcgcg acgagccgcc gggcgaggcc gatcggtgac
    10501 gggtggtgcg ccctcgccgg gttcgctcct tctgtcggtc gagtcgtcgt gcgacgagac
    10561 cgggatcgcc gtgatcgacg gcggccgccg gatcctgtcc aacgtcgtcg ccagccagac
    10621 cgcgctccac gccgcgagcg gcgggatcgt gcccgaggtc gcggcgcgcg cccacctccg
    10681 ctggatcgtg cccgtcctcg accaggcgct cgttgatgcg ggcgtgacgg tcgacgacgt
    10741 ggacgcggtc gccgtcaccc atggcccggg tctcgccggc tcgctcctcg tcgggatcaa
    10801 cttcgccaag acgatcgcct gggtccacga caagccgctc gtcggggtca accacctcga
    10861 gggccacctg tacgccgcgt ggttgaccga ccccggcgag gacgagcgcg aggagccgcg
    10921 gttcccgctc gtcgccctca tcgtgtcggg cggtcacacg ttcctcgtcg agatgcgcga
    10981 ccacctgacc taccgattgc tcggccagac ggtcgatgac gccgccggcg aggcgttcga
    11041 caaggtcggc cggctgctcg gcctgccgta tcccggcggg cccgcggtct cacgagcggc
    11101 cgagcgggcc gtgagacacg acgccgtgtt tccacgggcc tggctcggcc agacgtacga
    11161 cttcagcttc agcggcctca agaccgccgc ccgccggacg atcgacgaag cccgggcggg
    11221 cgaggggctg ccgaccggca aggccgccgc gtcggaaccg gacgcgcggc tgtcggaggc
    11281 cgccgttgcc gagctgtcgt ggggattcca ggacgccgtc gtcgacgtcc tcgtgaccaa
    11341 ggcgatccgg gcggccgagg agactggtgc ccgctcgatc gtgctcggcg gaggggtcgc
    11401 ctccaacgcc gcgctgcggg cacgcctggc cgaggaggcg gagtcgcgcg gcttgccgct
    11461 gatcgtgccc cgcccggcgc tctgcaccga taacggggcg atgatcgggg cggccggggc
    11521 gcggcggttc gcggcgggcg agcgggccga cctgtccctc ga
//
LOCUS       DATAIW010000011        11149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_59906, whole genome shotgun sequence.
ACCESSION   DATAIW010000011 DATAIW010000000
VERSION     DATAIW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59906"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="hutU"
                     /locus_tag="VHM48_00350"
     CDS             complement(<1..784)
                     /gene="hutU"
                     /locus_tag="VHM48_00350"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2753878.1"
                     /translation="MTSGPRPVRAPRGSELTCRGWQQEAALRMLMNNLDPEVAENPDA
                     LVVYGGTGRAARSWDAFDAIVRELRALADDETLLVQSGKPVAVFRTHEWAPRVLIANS
                     NLVGKWATWEVFRDLEAKGLMMYGQMTAGSWIYIGTQGILQGTYETFAELARQHFGGS
                     LAGRVVLTAGLGGMGGAQPLAVTMNDGVCLAIEVDETRARRRLDIGYVDRLTDSPAEA
                     LTWAREAAVAGRTESIALIGNAAEIEPAWAKGGERFDAVTDQT"
     gene            complement(825..1499)
                     /locus_tag="VHM48_00355"
     CDS             complement(825..1499)
                     /locus_tag="VHM48_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753879.1"
                     /translation="MNPRRAAVAARLIRLAIVAAGVAACGSTPGPTRAVLPTTLATPS
                     ASAAPAATASPAGGSPGASPVTGFDVVVDQGLLSFIPIGGNGLMQSVDPETTAQVAQD
                     PSLRANASGLFMAMYTLSAGTSSAGPGDDIAVVSVVRLRDPAADDAWFRDWRDSYDTA
                     ACANAGGVSRHAQTDIGTHTVFIGSCAGGSFTYHTRVADGAIVISITSIGPAHLGETV
                     MERLAP"
     gene            complement(1688..2467)
                     /locus_tag="VHM48_00360"
     CDS             complement(1688..2467)
                     /locus_tag="VHM48_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753880.1"
                     /translation="MTAIRIVDVTDEPSFRLIPPCADPGFDHRSCDYWEDSDRGSKAA
                     RLAWIERSTPSAATDRPALTDNPFAPARDTAVVNPFAPIARSSRPAFNPFGDDEDDGP
                     ADNPFAPPPPNRPSVGTDVPRKLGLLGRGRAVFGSYAKVLLVDDAPTVYAQFGPLSAY
                     PRAQRLRELYAQLPDAPLPAVITCISTTAAARGAGHAGRLVEAICDDLATRGFAAVEA
                     YPERGTRPDQTSAATPEFWLAAGFTLAVDDERFPVVRRELG"
     gene            complement(2464..4128)
                     /gene="hutH"
                     /locus_tag="VHM48_00365"
     CDS             complement(2464..4128)
                     /gene="hutH"
                     /locus_tag="VHM48_00365"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX2753881.1"
                     /translation="MTEPRGSSPTDPIVLTGADLSIADVEAVARRGRSATLDDAARGR
                     MQESRDLIERLVAAGEVVYGVTTGFGALASRFVEPADAKRLQENLLMSHAAGVGAPLE
                     TEIVRAMLLLRANTLALGHSGCRPELVDRLLGFLELGIHPVVPAQGSVGASGDLAPLA
                     HLALPLIGRGQVELHGQRLPGLVALREVGLEPLTLQAKEGLALLNGTQLMTAIGALLL
                     ADADRLGATASVAAAISVEALLGTDVAFSAAYQLARPHPGQIAVAAELRHLLRDSSVQ
                     TGHHGDAHKVQDPYSLRCVPQVHGAVRDALDHLRRVLDIEMNSATDNPLVFPEGGVAA
                     ADALATGGGRVISGGNFHGEPVALALDFAKIALAELGAISERRTSLLLDPRLNGGLPA
                     FLAPASGLDSGLMIVQYTAAALASENKVLAHPASVDSIPTSANQEDHVSMGPIAARHA
                     RTVLEHVERIVAIELLVGAEALDQRLELLADGYDAHPTPVPGAGVVLARDRIRSVVRP
                     LDGDREPGPDLAALTRLVHDGGLVDLVTSAGPTPGGGTSSLHGPER"
     gene            4245..4904
                     /locus_tag="VHM48_00370"
     CDS             4245..4904
                     /locus_tag="VHM48_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2753882.1"
                     /translation="MLAFIDKIVIPFLDGLYGAVGYLGVLIAMAIESAMIPLPSELIL
                     PYAGFLISDPTKLEPLTHGAWGFWVVVVVATIGNTIGSLIAYAIGAWGGRPFLQRYGK
                     YLLIRQHEIELAERFFDKYGAATAFFSRLLPIVRTFISFPAGVARMPLRKFIAYSTAG
                     AFLWSTLLVYAGMVLGSNWVRIREALQPFDLLIALGVVGLVVLFVWWRLGMPGRRRRG
                     V"
     gene            complement(4901..6223)
                     /locus_tag="VHM48_00375"
     CDS             complement(4901..6223)
                     /locus_tag="VHM48_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2753883.1"
                     /translation="MTAPLSAARTDSGSDAGLMASLRGLDPNGRLLFLTRVLRMFGYG
                     FLAVVLVLYLAALGIDALTIGAILTLTLVGDTLISLWLTTSADRIGRRRVLIAGSLLM
                     TGAGLVFAVTSWVPLLILAATIGVISPTGNEVGPFLAVEQAALSQATPDARRTPTFAW
                     YNLAGYVATAIGALSAGLLSQGLQAAGLADVDSYRAIVVGYAIVGLVMAVVFWRVGSA
                     VEAPVAPPADDGIARRLGLGVRSRGIVARLSALFALDAFGGGFIPQSLMAYWFHLQYG
                     VEPALLGGIFFGANLLAAVSSLSASRFAARFGLVNTMVFTHLPSNVLLILVPLMPNLA
                     LAIAVLLLRFSLSQMDVPTRQSYVMAVVEPGERSAAAGVTGIARTTGASISPILSAPL
                     VASASLTAVPFLLAGGLKIVYDLLLYRAFRSGHAPDERSSPKSSPPKA"
     gene            6271..6552
                     /locus_tag="VHM48_00380"
     CDS             6271..6552
                     /locus_tag="VHM48_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753884.1"
                     /translation="MSKPQTSHEPSPDELEHGSADHGLHHPGDRVDEAHGPDDHGETH
                     GHDDHADAEEALGPVDVQAWGALALGVGAGLLVVLCLVITTSLLSPAAI"
     gene            6626..7324
                     /locus_tag="VHM48_00385"
     CDS             6626..7324
                     /locus_tag="VHM48_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753885.1"
                     /translation="MATALRSRQPVRAGFLIAFALLASFGGVLTMPRPVMACSCAMNR
                     TMKENATPEQAIFTGTAGEQQARGVPVQVDRWLWGQGAAPVVWLAASSFGDGAACGIT
                     PPSKGSRWLWVAWLPGNNGDFGTGLCSPAGDLDTPEGQAMLADALTVFDAVDPPAPTS
                     AEPNAAPTFAEPNAAPNPAAAPDPVATARDRTTVTILAALLVGSLALFGGLVLVARRS
                     AGRADRNRGHDDRV"
     gene            complement(7321..9027)
                     /locus_tag="VHM48_00390"
     CDS             complement(7321..9027)
                     /locus_tag="VHM48_00390"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX2753886.1"
                     /translation="MSFPSDLEIARSVTPRPIMDVAHDLGLHDDEIEPYGHTKAKVTL
                     SAIERLEAERPRGKYVVVTAISPTPLGEGKSTTTVGLAQGLNKIGRKAAVCIRQPSLG
                     PVFGIKGGAAGGGYSQVIPMADFNLHLTGDVHAIGAAHNLAAAFLDNHLHHGNALGID
                     PFSILWPRVMDISDRAVRRVIVGLGGKENGVPRETEFVITVASEVMAVLALASDIHDL
                     RARLGRIVMATRKDGTAVTAEDLKVAGSMTALLTDAIKPNLLQTLEGGPAFVHCGPFA
                     NIAHGNNSIIADRLALATNEIVCTEAGFGADMGAEKFFDIKCRVSGLNPDAAVVVATV
                     RALKMHGGVGKIVAGKPLDPALLEENVEAVRAGAANLAKQIENVTIFGVPVVVAINSF
                     PTDTTAEVEAIRQVALAAGARDAVVATHFADGGDGATTLAQAVWAAASSGEANFQLLY
                     PDDAPLGEKILTIATKIYGAAAIELAPAAKKALKLYEELGMGHLPVCMAKTQYSLSHD
                     ASLKGVPSGFTIPIRDVRLSAGAGFITPLVGEMRTMPGLPSRPGGENIDIDANGNVVG
                     LF"
     gene            complement(9024..10001)
                     /locus_tag="VHM48_00395"
     CDS             complement(9024..10001)
                     /locus_tag="VHM48_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX2753887.1"
                     /translation="MPRGGARLLQGGPIAAEIRQAVSEDVAAYHGRHGRRPVLAIVIC
                     GRDAPSMVYLGQILRSCDKVGIEGRLVEVDGEAAEASLVRAIRDLNADPDVNGIIVQM
                     PLPPTIRLRSIVDAVDPAKDIDGIHPLNAGLLRLGYDGFLPATAHAAVEILRRSGIEI
                     EGKRAVVIGRSAVVGMPAAFLLVRENATVTVCHSRTRDLARHVKDAEIIVVAAGHPGL
                     IRGDMVRRGVVVVDVGINVVDGAIVGDVDFESVRRVASAITPVPGGVGPLTNALLVAH
                     LIRAALGQEAVAGAPGDAALVSSLAPGDSSTPAPATVSGDPAAASRGVR"
     gene            complement(10014..10691)
                     /locus_tag="VHM48_00400"
     CDS             complement(10014..10691)
                     /locus_tag="VHM48_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX2753888.1"
                     /translation="MANSTVGTSFRDLTIGAFVEELASAAPVPGGGSASAVAASLGAG
                     LVAMVASLSEGRPKYAAHGDLHHRSQTIGTRLAGWFLELADEDADAYAGFAAAMKLPK
                     DTDADRATRKRALSVAARRAAEVPMRTVEACLELVATTEALVGRSNVNASSDLDVAAL
                     LGEAAARGAAANVLINLPSVDDDSFSGEATARVMGLIDSIEQIASAVHSGVRSGEMRG
                     PLEPAPG"
     gene            complement(10849..>11149)
                     /locus_tag="VHM48_00405"
     CDS             complement(10849..>11149)
                     /locus_tag="VHM48_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753889.1"
                     /translation="PSDPAAKEPGMAMIRVEPVEVHVRTGWFDGSPREITWGDERLPI
                     TRLAAVREEAAAYPVITGPRTMFEVETARARFALTYQHRSRRWTITALDEERRAA"
BASE COUNT         1606 a         3918 c         4046 g         1579 t
ORIGIN      
        1 atgtctggtc ggtcaccgca tcgaaccgct cacccccctt cgcccaggcg ggctcgatct
       61 ccgccgcgtt cccgatcagg gcgatcgact ccgtccggcc ggccacagcc gcctcgcgcg
      121 cccacgtcag ggcctccgcg ggcgagtccg tcagccggtc gacatagccg atgtccaggc
      181 gacgccgggc gcgggtctcg tcgacctcga tcgcgaggca gaccccgtcg ttcatcgtca
      241 cggccagcgg ctgggcgccg cccatcccgc cgaggccggc ggtgaggacc acccgcccgg
      301 cgagcgagcc gccgaaatgc tggcgagcca gctcggcgaa cgtctcgtac gtgccctgca
      361 ggatcccctg ggtcccgatg tagatccacg accccgcggt catctggccg tacatcatca
      421 ggcccttcgc ctcgaggtcg cggaagacct cccaggtcgc ccacttcccc acaagattgg
      481 agttggcgat caggacccgc ggcgcccact cgtgggtccg gaagaccgcg accggcttgc
      541 cggactggac gaggagcgtc tcgtcgtcgg ccagcgcccg gagctcgcga acgatcgcgt
      601 cgaaggcgtc ccagctccgc gccgcccgac ccgttccgcc gtacaccacg agggcatccg
      661 gattctcggc cacctccggg tcgaggttgt tcatgagcat ccggagcgcg gcctcctgct
      721 gccagccgcg gcacgtcagc tcgctgccgc ggggcgcccg gacgggtcgc ggtccgctcg
      781 tcatcgcctg atcgtagccg ccgcccgccg cctcgtgacg aagatcacgg cgccaatcgc
      841 tccatcacgg tctcgccgag gtgggccggt ccgatcgagg tgatcgagat gacgatcgcg
      901 ccgtcggcga ccctcgtgtg gtaggtgaac gagccgccgg cgcacgaccc gatgaacacg
      961 gtgtgggtcc cgatgtccgt ctgggcgtgg cggctgacgc cgcccgcatt ggcgcaggcc
     1021 gccgtgtcgt aggaatcgcg ccagtcacgg aaccaggcgt cgtcggccgc agggtcgcgc
     1081 agccggacca cgctcacgac ggcgatgtcg tcacccgggc cggccgacga ggtccccgcg
     1141 ctgagcgtgt acatggccat gaacaggcca ctggcgttgg cgcggagcga cgggtcctgg
     1201 gcgacctggg ccgtcgtttc gggatcgacc gactgcatga ggccgttgcc gccgatcgga
     1261 atgaaggaca ggaggccctg gtcgacgacg acgtcgaagc ccgtgacggg cgaggccccg
     1321 gggctgccgc cggccgggga cgcggtcgcg gccggcgcgg ccgacgcgga aggggttgcg
     1381 agcgtggttg ggaggacggc ccgggtcggc ccgggtgtcg atccacacgc ggcgacgccc
     1441 gcggcgacga tcgccagacg gatcaggcgg gcggcgacgg cggcacggcg cgggttcaac
     1501 ctggcagccg ctcgggatcg gctccgggcc ggatgaacgc cgactcgtag aactggaggg
     1561 ggttgccctc cgggttgcca agggcctgga tcatcgacat ggggatgcct cgggtcgcga
     1621 tggtggctga cgcccggacg gtatcaccgg cggagggccg cggagcccgg ccggcggggc
     1681 gcgcccctca gccgagctcg cgcctcacga cgggaaagcg ctcgtcgtcg acggccaggg
     1741 tgaagcccgc cgccagccag aactcgggcg tcgcggcgct cgtctggtcc ggtcgcgtgc
     1801 cgcgctccgg ataggcctcg acggcggcga acccgcgggt ggcgaggtcg tcgcagatgg
     1861 cctcgaccaa ccgccccgcg tggccggctc cccgggcggc cgcggtggtc gagatgcagg
     1921 tgatcaccgc cggcagcggg gcatccggca gctgcgcgta gagctcgcgc agccgctggg
     1981 cgcggggata cgccgacagc ggcccgaact gggcatagac ggtcggcgcg tcgtcgacca
     2041 ggaggacctt ggcgtagctc ccgaagaccg cccgcccgcg accgaggagg ccgagcttgc
     2101 gcggcacgtc ggtgccgacc gacggccggt tcggcggcgg tggggcgaac gggttgtcgg
     2161 ccgggccgtc gtcctcgtcg tcgccgaacg ggttgaaggc cggccgcgac gaccgggcga
     2221 tcggcgcgaa gggattgacc accgccgtgt cgcgggccgg ggcgaagggg ttgtccgtga
     2281 gtgccggacg atcggtcgcg gcagaaggcg tcgaccgctc gatccaggcc aggcgggccg
     2341 ccttcgagcc gcggtcgctg tcctcccagt aatcgcacga acggtgatcg aacccgggat
     2401 ccgcgcacgg cgggatgagc cggaaggagg gctcgtcggt gacgtcgacg atccggatcg
     2461 ccgtcatcgt tccgggccgt ggagcgagct cgtacctccg ccgggggtcg ggccggcgct
     2521 ggtgacgaga tcgacgagcc caccgtcgtg gacgaggcgg gtcagggccg cgaggtcggg
     2581 tccgggctca cggtcgccgt cgaggggccg gacgaccgac cggatccggt cgcgggcgag
     2641 cacgacgccg gcgcccggga ccggcgttgg atgggcgtcg tatccgtccg ccagcagttc
     2701 caggcgctgg tcgagggcct cggcgccgac gagcagctcg atcgcgacga tccgctcgac
     2761 gtgctcgagc acggttcggg cgtggcgggc ggcgatcggg cccatcgaga catggtcctc
     2821 ctggttggcg ctcgtcggga tcgagtcgac cgatgccggg tgggcgagga ccttgttctc
     2881 ggaggccagg gcggcggccg tgtactggac gatcatcagc ccggaatcga ggccactggc
     2941 cggggcgagg aaggcgggca gcccgccgtt caaccgtgga tcgaggagca gcgacgtccg
     3001 ccgctcgctg atcgccccga gctcggcgag cgcgatcttc gcgaagtcca gcgccagcgc
     3061 gaccggctcg ccgtggaagt tgccgccgct gatcacccgg ccgccaccgg tcgccagcgc
     3121 gtccgcagcc gccacgcccc cttccgggaa gacgagcgga ttgtcggtcg cgctgttcat
     3181 ctcgatgtcg aggacgcggc ggaggtggtc gagggcgtcg cggaccgcgc cgtggacctg
     3241 cggcacgcag cgcagcgaat acggatcctg gaccttgtgg gcatcgccgt ggtgcccggt
     3301 ctggaccgat gaatcgcgca ggaggtggcg cagctcggcg gccaccgcga tctgaccggg
     3361 gtgcggccgg gccagctggt aggcggccga gaacgccacg tccgtcccga gcaacgcctc
     3421 gacgctgatc gccgctgcga cactggccgt cgccccaagg cgatccgcgt ccgcgagcag
     3481 cagcgccccg atggccgtca tcagctgcgt cccgttgagg agcgccagtc cctccttggc
     3541 ctggagcgtg agcggctcca gcccgacttc gcgaagggcg acgagaccgg gcaggcgttg
     3601 gccgtggagc tccacctggc cgcggccgat cagcggcaac gcgagatggg cgagcggcgc
     3661 gaggtcgccg gacgcgccga ccgagccctg ggccggcacg accgggtgga tcccgagctc
     3721 gaggaacccc aggaggcgat cgaccagctc cggacggcag ccgctgtggc cgagcgccag
     3781 cgtgttcgcc cggaggagga gcatcgcccg gacgatctcc gtctcgagcg gcgcgccgac
     3841 gcccgccgcg tggctcatca ggaggttctc ctggaggcgc ttggcgtcgg ccggctcgac
     3901 gaagcgcgag gccagcgccc cgaaccccgt cgtcaccccg tacacgacct cgcccgcggc
     3961 caccagccgc tcgatcaggt cgcgggattc ctgcatccgg ccgcgggcgg cgtcgtcgag
     4021 ggtggcggag cggccgcgac gggcgaccgc ctcgacatcg gcgatcgaca ggtcggcgcc
     4081 ggtgaggacg atcgggtcgg tcgggctgga acccctgggt tcggtcacga tcggaggata
     4141 gtcggctatt ctcggcccgt aacctcgggc cagcggcgtc gcggagctcc gccctggacc
     4201 tcggtgggcg aatcccgact caacgcagag gttgctgact ctccatgctt gcgttcatcg
     4261 acaagatcgt catcccgttc ctcgacggcc tgtacggcgc cgtcggctat ctcggggtcc
     4321 tgatcgcgat ggcgatcgaa tcggcgatga tcccgctgcc ctccgagctg atcctgccct
     4381 acgccgggtt cctcatctcc gacccgacca agctcgagcc gctgacgcac ggtgcgtggg
     4441 gtttctgggt agtcgtcgtc gtggcgacga tcggcaacac gatcggctcg ctcatcgcct
     4501 atgccatcgg cgcgtggggc gggcgtccct tcctccagcg ctacggcaag tacctcctga
     4561 tccgccagca cgagatcgag ctcgccgaac gcttcttcga caagtacggc gcggccaccg
     4621 cgttcttcag ccggctgctg ccgatcgtcc ggaccttcat cagctttccg gcgggggtcg
     4681 cccggatgcc gctcaggaag ttcatcgcct actcgaccgc gggcgccttc ctctggtcga
     4741 ccctgctcgt ctacgcgggc atggtcctcg gatcgaactg ggtcaggatc cgggaggcgc
     4801 tccagccgtt cgacctgctg atcgcgctcg gcgtcgtcgg cctcgtcgtc ctcttcgtct
     4861 ggtggcggct cgggatgccc ggtcggcggc ggcgcggggt ctaggccttc ggcggcgacg
     4921 acttcggcga cgaccgctcg tcgggagcgt ggcccgagcg gaacgcgcga tacagcagca
     4981 ggtcgtagac gatcttcagg ccgccggcga ggaggaacgg gaccgcggtc aggctcgcgc
     5041 tcgcgaccag gggcgccgag aggatcggcg agatcgaggc acccgtcgtc cgggcgatcc
     5101 cggtgacgcc cgccgctgcc gatcgctcgc ccggctcgac gaccgccatc acgtacgact
     5161 ggcgagtcgg gacgtccatc tggctcaggc tgaaccgcag gagcaggacg gcgatggcca
     5221 gtgcgaggtt cggcatcagc gggacgagga tgaggaggac gttcgagggc aggtgggtga
     5281 agaccatcgt gttgacgagc ccgaaccgcg cggcgaaccg agacgccgac agcgacgaca
     5341 ccgccgccag caggttcgcc ccgaagaaga tcccgccgag gagcgccggc tcgacgccat
     5401 attggaggtg gaaccagtac gccatcaggc tctgcggaat gaagccgccg ccgaaggcat
     5461 cgagcgcgaa cagggccgac aacctggcga cgattccccg cgaccgcacg ccgagtccga
     5521 gacggcgggc gatcccgtcg tcggcgggcg gtgcgaccgg cgcctcgacg gcgctgccga
     5581 cacgccagaa gacgacggcc atgacgagcc cgacgatcgc ataaccgacg acgatcgccc
     5641 gatatgagtc gacgtcggcg agccccgccg cctggagacc ctggctgagg agcccggccg
     5701 acagggcgcc gatcgcggtc gcgacatagc ccgcgaggtt gtaccaggcg aacgtcggcg
     5761 tccggcgggc gtcgggcgtt gcttggctca gcgccgcctg ctcgactgcg aggaacggcc
     5821 cgacctcgtt gccggtcggg ctgatcacac cgatcgtcgc cgcgaggatc aggagcggca
     5881 cccagctcgt gaccgcgaac acgagcccgg cgcccgtcat cagcagcgac ccggcgatca
     5941 ggacccgccg ccggccgatc cggtcggcgc tcgtcgtcag ccagagcgag atcagcgtgt
     6001 cgccgaccag ggtgagcgtc aggatcgcgc cgatcgtgag ggcgtcgatg ccgagcgcgg
     6061 cgagatacag gacgaggacg accgcgagga acccgtagcc gaacatccgc aggacacggg
     6121 tgaggaagag gaggcggcca ttcgggtcga ggccgcggag gctggccatg aggccggcgt
     6181 cggaaccgga atccgtccgg gcggcggaaa ggggcgcagt cacccgggaa gtctacgtgc
     6241 gagaatgcgc cgggaggtgc caccgccgcg atgagcaagc cccagacgag ccacgagccc
     6301 agcccggacg agctcgagca cggatccgcg gatcacggcc tccaccaccc gggcgaccgg
     6361 gtcgacgagg cccacggccc ggacgaccat ggcgagaccc acggccacga cgaccacgcc
     6421 gacgcggaag aagcgctcgg accggtcgac gtccaggcct ggggcgcgct cgcgctcgga
     6481 gtcggcgccg gcctcctggt cgtcctgtgc ctggtgatca cgaccagcct cctgtcgccg
     6541 gcggccatct gacctccgcc gcggaacacc cggccgccgg ctgacgtccg atcgtcgacc
     6601 atcgcccttc tgcgggagac ccttcatggc gaccgccctc cggtctcgcc aacccgtccg
     6661 tgccggtttc ctgatcgcgt tcgcgctcct cgcgtcgttc ggcggggtcc tgacgatgcc
     6721 ccgtccggtc atggcctgca gctgcgcgat gaaccggacg atgaaggaga atgcgacccc
     6781 cgagcaggcg atcttcaccg ggaccgcggg cgagcaacaa gctcgcggcg ttccagtcca
     6841 ggtcgatcgc tggctgtggg gccagggcgc tgccccggtc gtctggctcg ccgcgtccag
     6901 cttcggcgac ggcgccgcgt gcggcatcac tccaccttcg aagggcagcc gctggctctg
     6961 ggtcgcgtgg ctgccgggca acaacggcga cttcgggaca gggctgtgct cgcccgccgg
     7021 cgacctcgac acgccggagg gccaggccat gctggctgac gcgctcaccg tcttcgacgc
     7081 cgtcgaccca cctgcgccga cctccgccga accgaacgcg gcgccgacct tcgccgaacc
     7141 gaacgcggcg ccgaaccccg ccgcagcacc cgatccggtc gcgaccgcgc gcgatcgcac
     7201 gacggtcacc atcctggccg cgctgctcgt cggatcactt gccctgttcg gcggcctcgt
     7261 cctcgtcgcc cgccggtcgg caggccgtgc cgatcgcaac cgtggtcacg acgatcgggt
     7321 ctagaagagc ccgacgacat tcccgttggc gtcgatgtcg atgttctcgc cgcccggtcg
     7381 cgacgggagc ccgggcatcg tccgcatctc gccgacgagg ggcgtgatga agcccgcgcc
     7441 ggccgacaga cggacgtcgc ggatcgggat ggtgaagccg ctcgggacgc ccttgaggct
     7501 cgcgtcgtgg ctgagcgagt actgggtctt ggccatgcag accgggagat ggcccatccc
     7561 gagctcctcg tagagcttca gggccttctt cgccgccggt gccagctcga tggccgccgc
     7621 gccgtagatc ttcgtggcga tcgtcaggat cttctcgccg agcggggcgt cgtccgggta
     7681 gaggagctgg aagttcgcct cgccgctcga ggcggcggcc cagaccgcct gggcgagggt
     7741 ggttgcgccg tcgccgccgt cggcgaaatg ggtggcgacg accgcgtcgc gagccccggc
     7801 cgcgagcgcc acctggcgga tcgcctcgac ctccgcggtc gtgtcggtcg ggaaggagtt
     7861 gatcgcgacg acgaccggga cgccgaagat cgtgacgttc tcgatctgct tggcgaggtt
     7921 ggccgcgccc gcccggaccg cctcgacgtt ctcctcgagc agggccgggt cgagcggctt
     7981 gccggccacg atcttcccga cgccgccgtg catcttgagg gctcgcaccg tggcgacgac
     8041 gacggccgca tcggggttca gcccggatac gcggcacttg atgtcgaaga acttctcggc
     8101 acccatgtcg gctccgaagc ccgcctccgt gcagacgatc tcgttcgtcg cgagcgccag
     8161 gcggtcggcg atgatcgagt tgttgccgtg ggcgatgttt gcgaacggcc cgcaatggac
     8221 gaatgccggc ccgccttcga gcgtctggag caggttcggc ttgatcgcgt cggtcaggag
     8281 cgcggtcatc gaacccgcga ccttgaggtc ctccgccgtc accgccgtgc cgtccttgcg
     8341 ggtcgccatg acgatccggc cgagccgggc gcgaaggtcg tggatgtcgc tggcgagggc
     8401 gaggacggcc atcacctccg aggcgaccgt gatcacgaac tccgtctcgc gcgggacccc
     8461 gttctccttg ccgccgaggc cgacgatcac ccgccggacc gcccgatcgc tgatgtccat
     8521 gacccgcggc cagaggatcg agaacgggtc gatcccgagg gcgttgccgt gatggaggtg
     8581 gttgtcgagg aacgccgcgg cgaggttgtg ggccgctccg atcgcgtgga cgtcgccggt
     8641 caggtggagg ttgaagtcgg ccatcgggat gacctggctg tagccgccgc cagcagcgcc
     8701 acccttgatc ccgaacaccg gcccgagcga cggctggcgg atgcagaccg cggccttccg
     8761 gccgatcttg ttcaggccct gggcgagccc gacggtcgtc gtcgacttgc cctcgccgag
     8821 cggcgtcgga ctgatcgcgg tgacgacgac gtacttgccg cgcggtcgtt cagcctcgag
     8881 cctttcgatg gcggacaggg tcaccttggc cttcgtgtgg ccgtagggct cgatctcgtc
     8941 gtcgtgcagg ccgaggtcgt gggcgacatc catgatcgga cgcggggtga cggagcgggc
     9001 gatctcgaga tcggacggaa agctcatcga actcctcggc tggcggcggc ggggtcgccg
     9061 gacacggtgg caggcgccgg tgtcgacgaa tcgccgggcg caagggatga aacgagcgcg
     9121 gcgtcgcccg gtgctccagc gaccgcctcc tggccgagtg cggcccggat gagatgggcg
     9181 acgagcaggg cgttggtcag cggaccgacg ccaccgggaa ccggagtgat cgccgacgcg
     9241 acccggcgca cggactcgaa gtcgacgtcg ccgacgatcg ccccgtcgac gacgttgatc
     9301 ccgacgtcga caacgacaac gccgcggcga accatgtcac cgcggatcag gcccgggtgc
     9361 cccgccgcga cgacgatgat ctcggcgtcc ttgacatggc gggccaggtc gcgggtccgg
     9421 gaatggcaga cggtcaccgt cgcgttctcg cggacgagga ggaacgcggc gggcatgccg
     9481 acgaccgcgg accgcccgat caccaccgcc cgtttccctt cgatctcgat cccggagcgg
     9541 cggaggatct cgaccgcggc gtgggccgtc gccggcagga agccgtcgta gccgaggcgg
     9601 aggagccccg cgttgagcgg atggatcccg tcgatgtcct tggccgggtc gaccgcgtcg
     9661 acgatgctcc gtagccggat cgtcggtggc agcggcatct ggacgatgat cccgttgacg
     9721 tccggatcgg cgttgaggtc gcggatcgcc cggacgaggc tggcctccgc cgcctcaccg
     9781 tcgacctcga cgagccggcc ctcgatgccg accttgtcgc agctccgcag gatctggccc
     9841 aggtagacca tcgacggcgc gtcgcggccg cagatcacga tggccagcac cggtcgacga
     9901 ccgtgccgcc cgtggtacgc ggcgacgtcc tcgctcaccg cctggcggat ctcggcggcg
     9961 atcggcccac cctggagcag tcgggcacct ccgcgcggca cccgatcagg ccctcatccc
    10021 ggcgccggct ccagcggccc gcgcatttcg ccgctccgaa cacccgagtg gaccgccgac
    10081 gcgatctgct cgatgctgtc gatgagcccc atcacccgag cggtcgcctc gccgctgaac
    10141 gaatcgtcgt cgaccgacgg caggttgatc aggacgttcg ccgccgcgcc gcgcgccgcg
    10201 gcctcgccga ggagcgccgc gacatcgagg tcgctcgagg cattgacgtt gctccgcccg
    10261 acgagcgcct cggtggtggc gacgagctcg aggcacgcct cgaccgtgcg catcggcacc
    10321 tcggctgccc gcctggccgc gacgctcagc gccctcttcc gggtcgcgcg gtcggcgtcc
    10381 gtgtccttcg gcagcttcat cgcggccgcg aacccggcat aggcgtcggc gtcctcgtcg
    10441 gcgagctcga ggaaccatcc ggcaagccgc gtgccgatcg tctggcttcg atggtggagg
    10501 tcgccgtgag cggcgtactt cggccgccct tcggacaggg acgcgaccat cgccacgagc
    10561 cccgctccga gtgacgcggc aactgccgag gcgctgcccc ctcccggaac cggtgcggcg
    10621 gacgcaagct cctcgacgaa tgcgccgata gtcaggtcac ggaacgaggt gccgacggtc
    10681 gagtttgcca tgccccgatt gtaggttgcc gcgccccttg cggacccggc gatgcgcagc
    10741 cggtcggtga cggggcgagt gcggtaccgg cgcgctcgcc ccgcatccga caggtctcac
    10801 gcaggtgggc cgcctgctcg agacgcgtgg ccgttcggcc cccaggcttc aggccgcgcg
    10861 ccgctcctcg tcgagggccg tgatcgtcca ccggcgggag cgatgctgat aggtgagggc
    10921 gaaccgtgcc cgggcggtct cgacctcgaa catggtccgc ggcccggtga tgaccggata
    10981 cgcggcggcc tcttcgcgga cggcggcgag gcgcgtgatc gggagacgct cgtcccccca
    11041 ggtgatctct cgtgggctgc cgtcgaacca gccggtccgg acgtggacct cgacaggttc
    11101 gactctgatc attgccattc caggctcctt cgcggcggga tcgcttggg
//
LOCUS       DATAIW010000012         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_89728, whole genome shotgun sequence.
ACCESSION   DATAIW010000012 DATAIW010000000
VERSION     DATAIW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89728"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VHM48_00410"
     CDS             complement(<1..813)
                     /locus_tag="VHM48_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2753890.1"
                     /translation="MQELLETLDGWTAEGQPAGRAVVIRTFGSAPRPEGAVLIRADDG
                     RIAGSVSGGCVEGAAAGEIERARRDGHSRVIRYGISDEDAWDVGLACGGTIDVLVEPV
                     VASAAVTGARERGVAVATILPPDAPPSAFGRHESGDGAPPVASIVIRADGTLDGSLGL
                     AGDDVALSAAAAEALLRGTSRTVTIGDRQVFVEAFPKKPRLVIVGAVEVARSLAVYAK
                     ELGYEVVVVDGRASFATPERFPPDRVDRLVVGWPDEVAEEIGLGADDAVAVLT"
     gene            complement(866..2257)
                     /locus_tag="VHM48_00415"
     CDS             complement(866..2257)
                     /locus_tag="VHM48_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2753891.1"
                     /translation="MERHDDPAIVRAPWGPEVDGRRLLAESVGFGRALRSAGIQTDLG
                     AAVDFARALTLVDLGQRDTVRAAGQAVFIRRRDDREIYDRVFARWWRRRGRKRPATDG
                     PMAPAGDEEAAEGDEGVASPAGEEGSLGDDSNTIDGIPMAITADEDDDSEIDGIIAAP
                     TAYSSSELLRHREFDRMTGAELRDAERLVDLLEPRLEMRRTRRYELHHHGRLLAPRTM
                     FRRNIATGGQFTEWVWRRPTKRPRQIVVLCDISGSMERHSRLLLRFVQALSAASSVKT
                     ESFVFGTRLTRVTRMLRDRDRDRALAKIADSVNDWAGGTRIGESFRDFNLHWARRTLR
                     TSGVVIVVSDGWDRGDPALVATETARLRRNCHRLVWLNPLAGTPGYEPLAGGMRAAMP
                     YIDDFVPAGTVASLERLGEILAGIRSSDTRRSGEAAAGADAGNRHSSMAIFANPPAVI
                     GGTADPAAARPLS"
BASE COUNT          321 a          911 c          893 g          326 t
ORIGIN      
        1 ggtcaggacc gcgaccgcgt cgtcggcgcc gagcccgatc tcctcggcga cctcgtccgg
       61 ccagccgacg acaagacgat ccacgcggtc gggcgggaag cgctccggcg ttgcgaacga
      121 cgcccgaccg tcgacgacga cgacctcata ccccagctcc tttgcgtaca cggccagcga
      181 gcgggcgacc tcgaccgccc cgacgatcac gaggcgcggc ttcttcggga aggcctcgac
      241 gaagacctgg cggtcaccga tcgtcacggt ccgcgaggtg ccgcggagga gcgcctcggc
      301 agccgcggcc gacagggcga cgtcgtcgcc ggccaagccg agcgaaccgt cgagcgtgcc
      361 gtccgcgcgg atgacgatcg acgccacggg cggcgccccg tcgccggatt cgtgccgccc
      421 gaatgcgctc ggcggcgcgt cgggcggcag gatcgtcgcg accgcgacgc ctcgctcccg
      481 agcgccggtc acggcagcgg aggccacgac cggctcgacg agcacgtcga tcgttccgcc
      541 gcacgccagg ccgacgtccc atgcatcctc gtcgctgatc ccgtagcgaa tcacccggct
      601 gtgcccgtcg cggcgagccc gttcgatctc gccggccgcc gcgccctcca cgcagccgcc
      661 gctgaccgag cccgcgatcc ggccgtcgtc ggcccggatc aggaccgcac cctcgggtcg
      721 cggcgcgctg ccgaacgtgc ggatcacgac ggcgcggccg gccggctggc cttcggccgt
      781 ccagccgtcg agggtctcga ggagttcctg catcggttcg tgcctgcctt ccggggctgg
      841 ttcgcggggt cgcgggtcgc tgccgtcagc tgaggggtcg ggcggcagcc gggtcggccg
      901 tgccgccgat gaccgcgggc ggattggcga agatcgccat gctcgaatgg cggttgccgg
      961 cgtcggcgcc ggccgccgcc tcaccgctgc ggcgcgtgtc ggacgagcgg attccggcga
     1021 ggatctcgcc gagccgctcg aggctggcga ccgtgccggc cgggacgaag tcgtcgatgt
     1081 acggcatcgc cgcacgcatc ccgccggcca gcggctcgta gcccggcgtg cctgccagcg
     1141 ggttgagcca gaccagacga tggcagttcc gccggagacg cgccgtctcg gtcgcgacga
     1201 gcgccgggtc gccacggtcc cagccgtcgg acacgacgat cacgacgccc gacgtccgga
     1261 gcgtccggcg cgcccagtgc aggttgaagt cccggaacga ctccccgatg cgcgtcccgc
     1321 cggcccagtc gttgacgctg tcggcgatct tggccagggc ccggtcgcga tcccggtcgc
     1381 ggagcatccg cgtcacccgg gtcaggcggg tcccgaagac gaacgactcg gtcttgaccg
     1441 acgaggcggc cgacagggcc tggacgaacc ggaggagcag ccgcgagtgg cgctccatcg
     1501 agcccgagat gtcgcacagg acgacgatct ggcgcggtcg cttcgtcggc cgacgccaga
     1561 cccactcggt gaactggccg ccggtcgcga tgttccgccg gaacatcgtc cgcggggcga
     1621 gcaggcggcc gtgatggtgg agctcgtagc ggcgcgtccg gcgcatctcg agccggggct
     1681 cgagcaggtc gacgagacgc tcggcgtcgc gcagctcggc cccggtcatc cggtcgaact
     1741 cgcggtggcg gaggagctcg ctcgaggagt acgccgtcgg cgccgcgatg atcccgtcga
     1801 tctcgctgtc gtcgtcctca tcggcggtga tcgccatggg aatgccgtcg atcgtgttgc
     1861 tgtcgtcgcc gagcgagccc tcctcgccgg cgggcgatgc gacgccctcg tcgccctcgg
     1921 cggcctcctc gtcaccggcc ggggccatcg ggccgtccgt cgccgggcgc ttccggccgc
     1981 gtcgccgcca ccatcgggcg aacacccggt cgtagatctc gcggtcgtcg cgccgccgga
     2041 tgaacaccgc ctggcccgcg gcgcggaccg tgtcgcgctg gcccaggtcg acgagggtca
     2101 gggcccgggc gaagtcgacc gcggccccga ggtcggtctg gatcccggcc gaccggagcg
     2161 cccgtccgaa gccgacggat tcggcgagca ggcgccggcc gtcgacctcc gggccccacg
     2221 gcgcgcgaac gatcgccggg tcatcgtgac gctccatgcg gagactgtgc cacgagtcgt
     2281 ggccccgtgc gcgatcaggc gtctcgggcg gtcgagagca ggcctgcccg caccagatcc
     2341 gcgtcgtagg cgaacgcccg ccggatgccg ccgcgcgcgg cgaacgtgag cgtcacccga
     2401 tctctgatat cagtaccttg gatcagctga cgctgagtcg gccctcgtgc g
//
LOCUS       DATAIW010000013        11733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_317, whole genome shotgun sequence.
ACCESSION   DATAIW010000013 DATAIW010000000
VERSION     DATAIW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1378)
                     /locus_tag="VHM48_00420"
     CDS             complement(35..1378)
                     /locus_tag="VHM48_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX2753892.1"
                     /translation="MAIFPRLRLRDPAIDLVDLPWELPLEAWSADRLAFRYVPVGPSR
                     HLVRFLVSSGILYALKELPLGVARQEYDVLLHLENLGLPTVEAAGVAEAPARDSAILV
                     TKFLPHSLQYRRLMMRLPPTSSSYRERLLDAMAFLLVDLHRSGVFWGDCSLANTLFRR
                     DGDLIQAYLVDAETSHVFPSLSDGQRAYDLEILVENVAFGLADLAAMQGWPDDSDDAI
                     GAAEHVRTRYEQIWAMIHDEPVLHPDDRQAIRNRVRRLNELGYSVDLDVDPDADDGRV
                     RLRMSVTTRRYHAHELERRTRIRALEGQARLLLNDLNEYGAWLEWSESRAIAGEEVAD
                     RWLHDVYRPTLARIAAAVGPARDLVQAYCDVLEEKWLLSEQHHRDIGLATAIDTYLEL
                     GAPAPESLGRGDDATTLLDPLDVDPLELDPFDVDGDPTDGNADGGSGPGMAPSGT"
     gene            complement(1437..1877)
                     /locus_tag="VHM48_00425"
     CDS             complement(1437..1877)
                     /locus_tag="VHM48_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2753893.1"
                     /translation="MTDRQPVTLRAATPDDAERIAALFTEEGYPSGPSDIVERLARFD
                     SEYSQVIVADNDGVVLGFVAVHALPRFEHSDRIVRIMALVVDAGERGRGIGRLLMEEA
                     ERLGREVDAAFAEVTAGHHRPDARRLYEELGYDGTVAAYLRKRL"
     gene            complement(1874..2374)
                     /locus_tag="VHM48_00430"
     CDS             complement(1874..2374)
                     /locus_tag="VHM48_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753894.1"
                     /translation="MVHRDGKRADEGAGSAILWLDEGRSPQVLAVIWATIELERALGD
                     LGPTPDAAGATVSAAGAVEDPFLGARVVVVPSHENGIRIALAEPSTEGRLAATLARNG
                     EGPAGRYVLAPFALAVVEARAAAANVVLSKPASGPFGPAVLVLGGPAAGPHLILVDPA
                     AVPSSP"
     gene            complement(2377..3258)
                     /locus_tag="VHM48_00435"
     CDS             complement(2377..3258)
                     /locus_tag="VHM48_00435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2753895.1"
                     /translation="MAAPIPIAPSGQPGPPGRPADLTADDVRWLAWHEARSHALIGRE
                     VRDLGDAVLLFDDTDREPFWNRLAGIAWPADAAAFDVRLTEALGLFAILDRIPHVWSM
                     PGFDTPSDLTARLLANGFEDYGGGLVMALDPARSGPTAVLDAAAGVTVERLHRISGQP
                     AADGARAIAGVLLASFAVEPERRVAIELEAVQGLATEAYHAILIRVDGVPAAVARRTT
                     FAGATYLSSIGTDPAFRGRGLGRFVTALAVADALADRSRWTYLGVFEDNAVARSLYGS
                     LGFVTVGGPAPDLLLRP"
     gene            3397..3822
                     /locus_tag="VHM48_00440"
     CDS             3397..3822
                     /locus_tag="VHM48_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2753896.1"
                     /translation="MRRLALSLLAIFAIVGLAACGGGATPTPASSSALTPPSAAGSAA
                     AGAACATAAAGATATVNVSIKDFKFSPQPVQAKVGDVVAWKNDDTAPHSATMDAGTCD
                     TDTITTGSSAMLVFTAAGTYTYHCKIHPGQMKDFTVEVK"
     gene            complement(3907..4929)
                     /locus_tag="VHM48_00445"
     CDS             complement(3907..4929)
                     /locus_tag="VHM48_00445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2753897.1"
                     /translation="MGQVDRFEELAASVGGFYRSWVIYLGLELGMFARIRAADAAGMS
                     ASELASAAGCGPEPTEAWVRAAHAAGLIELDGGRATLAADVAAVLLDDTHPEYLGGQF
                     VVAVVSSLDYIGLADFFRSGRTIAERPPRFHRAIEAVTVQDIAVFFQEGLAELPDLAA
                     ALNRGGRVLDVACGGGRWLIAVARRFPATQLVGVEFEPDSVERATRQVRDAGLDGRIR
                     VEARTIPDMPFRSEFDLVYLQDALHELPDPAPSLRAAWAAVAPGGRLVVLEWCLPDDP
                     RDGSDPPSLQEELLWGIQIDELYQGTRMYTHDGYDALFASAGVPAPAVVELRSGASLF
                     VVEQPG"
     gene            5091..5642
                     /locus_tag="VHM48_00450"
     CDS             5091..5642
                     /locus_tag="VHM48_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2753898.1"
                     /translation="MQLAARLATDLDGSFEALVLAHQDRLFTIALRTRGDRHDAEELV
                     QDAFVRAYRALAAWPTTRIRELRIRGWLTTILLNAGRNRARVKRVPTTELAFDPGAEP
                     TADPLARRDERETWARLLTGLSPAQRTAVVLRHVDGMSYAEIAVAVGRPEGTVKAHVH
                     RGLAALRTALLAAERLEREEIPA"
     gene            5639..6436
                     /locus_tag="VHM48_00455"
     CDS             5639..6436
                     /locus_tag="VHM48_00455"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2753899.1"
                     /translation="MTDMIDPASAAALAGLREPAPPGLAHSTLVALGLADDYAVIPSP
                     VGDLRVAWNGRGVSEVAGASDDQTFEARFHARTGRRAQRRDALPTDLARKIERRLGGD
                     RHNRIDLDLRGSTAFEQAVWLKALEIPRGEVRPYGWIAAEIGRPKAVRAVGTALGHNP
                     VPLIVPCHRVVRSDGMIGQYSLGGPQNKRTILADEGADPDGLEALGRAGVRYFGSDTT
                     HIYCMPTCRDARRVTERHLVHFPSAKAASAAGYRPCMHCRPLGAAVA"
     gene            6534..7514
                     /locus_tag="VHM48_00460"
     CDS             6534..7514
                     /locus_tag="VHM48_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2753900.1"
                     /translation="MPTSAHSRPASAVLVWTAIVILYVVWGSTYLGIRVAIGSIPPFV
                     MAAVRFAIAGAVMLTIVAVVRRGQITRPTLQEWRDTCIIGALLMGGGMGAVAWGELTV
                     PSGIAGVLIAMMPVWIAVFGRIFLGERLPGLAVVGIATGMVGVAILVGSGVVIDRSLD
                     PAGIAALIFSPMSWAAGSLFSAHRARLPRDPFLTTGMEMIAGSIVLAIAAFLTGEVAT
                     FRPEAVTGESLLALVYLTAIGSLVAFTAFAWVLRQAPLPLIATYAFVNPVVAVFLGWL
                     ILHEAVTRIQFVAGAVIVVGVALLILARGRMRAPQPARRPIADRDRSAAA"
     gene            complement(7592..9661)
                     /locus_tag="VHM48_00465"
     CDS             complement(7592..9661)
                     /locus_tag="VHM48_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2753901.1"
                     /translation="MSSQPPTIPPGWSVADLATLAELAETFVRGGSVRRSNLAAEALA
                     RAADPAQVRQLRLVLRLFESRLANLLLTGRPIAFRDRTPADRERVLLAWANSRLPLRR
                     SAYQAFRKLLTFLAYADPGPPGIANPLHAAIGYIPERPPVTALLAAIRPLDIHGPPTG
                     ADDAVQLDADVVVVGSGAGGGVIAAELARAGRSVVVVEAGPFLDEAHLPTDELDAYSR
                     VYLNHGLLTTWDGAITMLAGTAVGGGTLVNWMTSIAPPDDVRAEWASDHGIDGIDGAE
                     FDADLQTIEVELGVAPATAIPPKDAVILRGAANLGWEAAPIRRNATDCGDCGSCPFGC
                     PRGAKQSGMRAHLATAVAAGARVVDRARVTRVLIVHGRVVGVEADVLVVDPATGEPAL
                     GPGGDPLRPRTRRLVVRTAQVVVAAGALRSPAVLLASGLDHPAIGRHLRLHPAPVVAG
                     LFDDPIEMWRGTMQAARSLEFSRPAGARNGYVIESAPGHPGLIALALPWEGTDAHADL
                     LGRIRHVAPLIAVTRDGGAGRVRLAGSGRARIDYALDERGVATLRHAMVSMAGIVRAA
                     GARQIVAVGTPPRWHGRSGFAPGQEERSFVVFEDGLRSFDFGANRGSVFSAHQMGSVR
                     MGAAVNDHPCDPAGRVRTRSGAPIPGLYVGDGSLFPTGLGVNPMITIMALARRVARTV
                     AAEVRSG"
     gene            complement(9771..9983)
                     /locus_tag="VHM48_00470"
     CDS             complement(9771..9983)
                     /locus_tag="VHM48_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753902.1"
                     /translation="MILVRDLRLVGFAVVIVVLIAATAFGLWHVVVGGLLNGNPRAAG
                     FGVVLAAAAGSLLAAAILGHRRRRTV"
     gene            complement(9980..11215)
                     /locus_tag="VHM48_00475"
     CDS             complement(9980..11215)
                     /locus_tag="VHM48_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2753903.1"
                     /translation="MVDFQLTDENRLVQQAARAFAEAEILPHIREWDEKGEVHRELFA
                     KMAEQGFLGAPIPEAWGGSGMDYLSFAILCEELERADTAFRVVQSVHVGLNSLALLQW
                     ATDEQKERWLAPQARGEKLATFGLTEPGVGTDAANLATTARRDGAAYRLNGQKIWISL
                     ADLADHFLVFASVDRSKGWRGVTAFLLERGMAGLTTGTLHGKLGIRAGNTGLINLDDC
                     VVPADHRIGEEGEGFLIAMSAIDQGRFTVAAGAVGLAQACLDASLKYAHERSTFGQEI
                     GRHQLVQQLIAKMAAGTEMGRLLVWRAAWLKNQGLRNTRETSLAKWHATEHAVQSALD
                     AIQVHGANGYSNEFPVERYLRNSKAAVIYEGTSQLHTIIQGEYALGYRSDRPLRCEPL
                     PAQGWERPGAGAGAVAGRA"
     gene            11410..>11733
                     /locus_tag="VHM48_00480"
     CDS             11410..>11733
                     /locus_tag="VHM48_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2753904.1"
                     /translation="MTDIAVPAALPADLSTVADHITPVLGRYFERTWSHGEGHRLYDS
                     GGKAYLDFANGIAVTALGHHHPTVTAAIHAQVDRLIGPTGAMGFAEPVVRLADMLAET
                     LPAPID"
BASE COUNT         1663 a         4253 c         4249 g         1568 t
ORIGIN      
        1 cggatgacgg gatccgcggc ggtcgatggc gtcgtcacgt ccccgatggt gccattcccg
       61 gccccgatcc gccgtccgca ttcccgtccg tgggatcgcc gtcgacgtcg aatgggtcga
      121 gttcgagcgg gtcgacgtcg agcgggtcga ggagcgtcgt cgcgtcgtcg cccctgccga
      181 gcgactcggg ggccggtgcc ccgagctcga ggtaggtgtc gatcgccgtc gcgaggccga
      241 tgtcgcgatg gtgctgctcg gacagcaacc acttctcctc gaggacgtcg cagtacgcct
      301 ggacgagatc acgagccggc ccgacggccg ccgcgatccg ggcaagggtc ggtcggtaga
      361 cgtcgtgcag ccagcggtcc gcgacttcct cgccggcgat cgcccggctc tcgctccact
      421 cgagccacgc cccgtactcg ttgaggtcgt tgaggagcag gcgggcctgc ccttcgagcg
      481 cccggatccg cgtccggcgc tcgagctcgt gggcgtggta gcgccgcgtc gtgaccgaca
      541 tccggaggcg gacgcgcccg tcgtcggcgt ccggatcgac gtcgaggtcg accgagtagc
      601 cgagctcgtt caggcggcgg acccggttgc ggatcgcctg gcgatcgtcc gggtggagga
      661 cgggctcgtc gtggatcatc gcccagatct gctcgtatcg ggtgcggacg tgctcggcgg
      721 ccccgatcgc gtcgtcgctg tcgtcgggcc agccctgcat cgccgcgagg tcggcgaggc
      781 cgaaggccac gttctcgacg aggatctcga ggtcgtacgc ccgctggccg tccgacagcg
      841 acggaaagac gtggctcgtc tcggcatcga ccaggtaggc ctggatcagg tcgccgtccc
      901 gccggaacag ggtgttcgcc agcgagcagt cgccccagaa cacgccgctc cggtggaggt
      961 cgacgaggag gaatgccatc gcgtcgagga gccgctcgcg gtaggaactc gaggtcggcg
     1021 gcaaccgcat catcaggcgc cggtactgga gggagtgggg caggaatttc gtgacgagga
     1081 tcgcgctgtc gcgggccggc gcctccgcca cgccggccgc ctcgaccgtc ggcaggccga
     1141 ggttctcgag gtggagcagg acgtcgtatt cctggcgggc gacgccgagc gggagctcct
     1201 tcagggcgta gaggatcccg ctcgagacga ggaaccggac gaggtggcgg gacggcccga
     1261 ccgggacgta gcggaaggcg aggcggtcgg ccgaccacgc ctcgagcggg agctcccacg
     1321 gcaggtcgac gaggtcgatc gccggatcac gaaggcggag gcgtggaaag atggccatcg
     1381 ttcgtgctgg gtcggggcgc gtggcgcggt cgccggctcg gcagccggtg cggccgctag
     1441 agacgcttcc ggagataggc cgcgaccgtc ccgtcgtagc cgagctcttc gtacaggcgc
     1501 cgggcgtccg ggcgatggtg gccggcggtg acctcggcga aggccgcgtc gacctcccgc
     1561 ccgagccgct cggcctcttc catcaggagc cggccgatcc cccgaccgcg ctcgccggcg
     1621 tcgaccacga gggccatgat ccggacgatc cgatcggagt gctcgaagcg cggcagggca
     1681 tggacggcga cgaagccgag gaccacaccg tcgttgtcgg caacgatcac ctgggagtat
     1741 tccgagtcga accgggcgag gcgctcgacg atgtcgctcg ggccggacgg atagccctcc
     1801 tcggtgaaca gggcggcgat ccgctcggcg tcgtcgggcg tggccgcgcg gagggtgacg
     1861 ggctggcggt cggtcatggc gacgatggta ccgccgccgg gtcgaccagg atcaggtgcg
     1921 ggccggcggc cggcccgccc aggacgagga ccgccggtcc gaacggtccg cttgccggct
     1981 tgctgagcac gacgttcgcc gccgcggcac gtgcctcgac gacggccagc gcgaacggcg
     2041 ccaggacgta ccggccggcc gggccctccc cgttgcgggc cagggtcgcg gccaggcgtc
     2101 cctccgtcga gggctccgcc agcgcgatcc ggatcccgtt ctcgtgtgac ggaacgacaa
     2161 cgacgcgagc cccgaggaac ggatcctcga cggcgccagc cgccgagacc gtggcgcccg
     2221 cggcgtccgg ggtcggtccg aggtcgccca gcgcgcgctc gagctcgatc gtcgcccaga
     2281 tcaccgcgag cacctgcgga gaccggccct cgtcgagcca caggatggcc gacccggccc
     2341 cttcgtcggc ccgcttgccg tcccgatgga ccaccgtcag ggccgaagca acaggtccgg
     2401 cgccgggccg ccgaccgtca cgaacccgag cgagccatag agcgagcgcg cgacggcgtt
     2461 gtcctcgaag accccgagat acgtccagcg gctgcggtcc gcgagcgcgt cggcgacggc
     2521 gagggcagtc acgaaccggc cgaggccgcg gccgcggaag gccggatccg tgccgatcga
     2581 cgagaggtag gtggcgcccg cgaaggtcgt ccgtcgggca acggcggccg gcaccccgtc
     2641 gacccggatg aggatcgcgt gataggcctc ggtcgcaagc ccctggacgg cctcgagctc
     2701 gatcgcgaca cgccgctcgg gctcgaccgc gaacgacgcg agcaggaccc ccgcgatggc
     2761 tcgtgcgccg tcggcggccg gctggccgct gatccggtgg agccgctcga cggtgacgcc
     2821 ggctgccgcg tccagcacgg cggtcggccc ggatcgtgcg ggatcgaggg ccatgacgag
     2881 tcccccaccg tagtcctcga atccgttggc gaggaggcgc gccgtcaggt cggagggcgt
     2941 gtcgaagccg ggcatcgacc agacatgcgg gatccggtcg aggatcgcga acaggccgag
     3001 cgcctcggtc aggcggacgt cgaacgcggc ggcatccgcg ggccacgcga tcccggcgag
     3061 ccggttccag aacggctcgc ggtccgtgtc gtcgaacagg agaaccgcgt cgccgagatc
     3121 ccggacctct cgcccgatga gcgcatgcga gcgagcctcg tgccacgcga gccagcggac
     3181 gtcgtcggcg gtcaggtccg cgggccggcc gggaggaccc ggctgaccgg atggcgcgat
     3241 cgggatcggg gcggccatgg ccggaacgat acccgtcacg ctccggcgcg cggagccgtc
     3301 gaccgaaacc actggcgtcg ccgcgacgta gggttgacca ttggcccacg atcggcttcg
     3361 cccccgcccg atcaggccaa cggaggatgc ccacccatgc gacgccttgc cctctccctg
     3421 ctcgcgatct tcgcgatcgt cggcctcgcc gcctgtggcg gcggcgcaac cccgaccccg
     3481 gcctcgagca gcgcgctcac tcccccgtcg gcggcgggca gcgcggccgc cggagccgcc
     3541 tgcgccacgg ccgccgccgg agcgaccgca accgtcaatg tctcgatcaa ggacttcaag
     3601 ttcagccccc agccggttca ggccaaggtc ggcgacgtcg tcgcctggaa gaacgacgac
     3661 accgcgccgc acagcgccac gatggatgcc ggcacctgcg ataccgacac gatcaccacc
     3721 ggatcgagcg cgatgctcgt cttcaccgcc gccggcacgt acacatacca ctgcaagatc
     3781 catccgggtc agatgaagga cttcaccgtc gaggtgaagt agggtcgggt cgggagcgac
     3841 ggacccgatc gggggcccgg gtcgcccggg tcagcaggtt cacccgggtc agccgggtcg
     3901 cccgggtcag ccgggttgct cgacgacgaa gagcgacgca ccggatcgga gctccacgac
     3961 cgccggtgca gggacgccgg cagacgcgaa cagggcgtca tagccgtcgt gggtgtacat
     4021 ccgcgtcccc tggtaaagct cgtcgatctg gatgccccag agcagctctt cctggagtga
     4081 cggcggatcg cttccgtcgc gcggatcgtc cggcaggcac cattcgagga cgaccagccg
     4141 gccgccgggt gccaccgcgg cccaggccgc tcgcagcgat ggggccggat cgggcagctc
     4201 gtgcagggca tcctggaggt acacgaggtc gaactcgctc cggaacggca tgtcggggat
     4261 cgtccgggcc tcgacccgga tccggccgtc gaggccggca tcgcgcacct ggcgggtcgc
     4321 ccgctcgacc gagtcgggct cgaattcgac gcccacgagc tgggtcgccg ggaaccggcg
     4381 ggcgacggcg atcaaccacc ggccgccgcc gcaggcgacg tccaggacgc gcccgccgcg
     4441 gttgagtgcg gccgcaagat cgggcagctc ggcaagccct tcctggaaga acaccgcgat
     4501 gtcctggacc gtgaccgcct cgatcgcccg atggaaccgc ggcggtcgtt cggcgatcgt
     4561 ccgtccggaa cggaagaagt cggcaagccc gatgtaatcg aggctcgaga cgaccgcgac
     4621 gacgaactgg ccgccgaggt actccggatg ggtgtcgtcg aggagaaccg cggcgacgtc
     4681 ggcggcaagc gtcgcacgtc cgccgtcgag ctcgatcagg ccggcggcgt gagccgcgcg
     4741 gacccacgcc tcggtcggtt ccggcccgca gcccgcggcc gatgcgagct cgctcgcgct
     4801 catgccggcc gcgtccgcgg cgcggatgcg ggcgaacatc ccgagctcga gcccgagata
     4861 gatcacccag ctccggtaga agccaccgac gctggccgcg agctcttcaa agcggtcgac
     4921 ctggcccatg gtgccgattg tcgcacggcc gacgacacgt ccccggatcg gcgacgatgc
     4981 gccggggacg ccccgcggca ccgatcggga tggggtcgcg acggtcctgc aaccatcccc
     5041 gcgatcgccg tgttccgaag ggcatgatgg acgaacgctc gcctgccgac gtgcagctcg
     5101 ccgcccgcct cgcgaccgat ctcgacgggt cgttcgaggc ccttgtcctg gcccaccagg
     5161 accgcctctt cacgatcgcc ctgcggaccc gcggcgatcg ccacgacgcg gaggagctcg
     5221 tccaggacgc cttcgtccgc gcgtaccggg ccctcgccgc atggccgacg acgcggatcc
     5281 gcgagctgcg aatccgcggc tggctcacga cgatcctcct gaacgccggc cgcaaccggg
     5341 cccgggtcaa gcgagttccg accaccgagc tggccttcga ccccggcgcc gagccgaccg
     5401 ccgacccgct cgcccgacgc gacgaacgcg agacgtgggc acgcctgctg acggggttga
     5461 gccccgccca gcggacggcc gtcgtcctcc gccacgtcga cggaatgtca tatgccgaga
     5521 tcgcggtcgc ggtcggccgg cccgaaggca ccgtcaaggc ccacgtccac cgcggactgg
     5581 ccgccctgcg cacagcgctc cttgccgccg agcggctcga gcgcgaggag atccccgcat
     5641 gaccgacatg atcgatcccg ccagcgcggc cgccctggcc gggctccgcg agcccgcgcc
     5701 cccgggtctt gcccattcga cgctcgtcgc gctcgggctc gccgacgact acgccgtcat
     5761 cccatcgccg gtcggcgacc tgcgggtcgc ctggaacggc cggggcgtgt ccgaggtggc
     5821 cggggcgtcc gacgaccaga cgttcgaggc ccgcttccac gcccggaccg ggcgccgtgc
     5881 tcaacgccgc gacgccctcc cgaccgatct ggcccggaag atcgagcggc gactcggcgg
     5941 cgaccgccac aaccggatcg acctcgacct gcgcggctcg accgcgttcg aacaggctgt
     6001 ctggctgaag gccctcgaga tcccgcgggg cgaggtccgg ccgtatggct ggatcgccgc
     6061 cgagatcggc cggccgaagg cggtccgggc cgtcgggacg gcgctcggcc acaaccccgt
     6121 cccgctcatc gtcccgtgcc accgggtcgt ccgcagcgac gggatgatcg gccagtactc
     6181 gctcggcgga ccgcagaaca agcgaacgat cctggccgac gagggcgccg atcccgacgg
     6241 cctcgaggcc ctcggccggg ccggcgtccg ctacttcggc tcggacacga cccacatcta
     6301 ctgcatgccg acgtgtcgcg acgcacgccg ggtcacggag cgccacctcg tccacttccc
     6361 gtcggcgaag gcggcatcgg cggccggcta ccggccgtgc atgcactgcc ggccgctcgg
     6421 agccgcggtg gcctgagctc gcccacggca ggcttgacga ggaggcccgg tggaacggcg
     6481 tggcgggtgg ccgacgctcg tcgtacactg cccgtacttt ggaccagtca actatgccaa
     6541 cgagcgcgca cagccgaccg gcgtcggcgg tcctcgtgtg gaccgcgatc gtcatcctgt
     6601 acgtcgtctg gggctcgacc tacctcggga tccgggtcgc catcggctcg atcccaccct
     6661 tcgtgatggc cgccgtccgg ttcgcgatcg ccggcgcggt gatgctcacc atcgtcgccg
     6721 tcgtccgccg cggacagatc acccgcccca ccctgcagga gtggcgcgac acgtgcatca
     6781 tcggcgcgct tctcatgggt ggcggcatgg gcgcggtcgc ctggggcgag ctgaccgtgc
     6841 cgtccggcat cgccggcgtc ctgatcgcga tgatgccggt ctggatcgcg gtcttcggac
     6901 ggatattcct cggcgagcgc ctgccggggc tggcggtggt cggcatcgcg accgggatgg
     6961 tcggtgtcgc gatcctcgtc ggatccgggg tggtcatcga ccgcagcctc gaccccgcag
     7021 gcatcgccgc cctgatcttc tcgccgatgt cctgggcagc cggctccctg ttctcggccc
     7081 atcgggcccg gctgccccgc gacccgttcc tgacgacggg catggagatg atcgcgggct
     7141 cgatcgtgct ggcgatcgcg gcgttcctga ccggtgaggt cgcgacattc cgtccggaag
     7201 cggtgaccgg cgagtcgctc ctcgccctcg tctacctcac ggcgatcggg agcctcgtgg
     7261 ccttcacggc gttcgcctgg gtgctccgcc aggctccact gcccctgatc gcgacgtacg
     7321 ccttcgtgaa tccggtggtc gcggtcttcc taggctggct gatcctgcac gaggccgtga
     7381 cccggatcca gttcgtcgcc ggggcggtca tcgtcgtcgg cgtcgccttg ctgatcctgg
     7441 cgcggggacg gatgcgcgcc ccgcaaccgg cccgtcgacc gatcgccgac cgggaccgct
     7501 cggccgcggc ctgaccagcg gagcgatcct gcgcagccct cgacctcgcc cgttcgatcc
     7561 tggccgattc ggggccaggt cggtcagcgc gtcagcccga ccgcacctcg gcagccaccg
     7621 tccgcgcgac gcgtcgggcg agggccatga tcgtgatcat cgggttgacg cccagcccgg
     7681 tcgggaacag cgagccgtct ccgacgtaga gacccgggat cggcgcgccg ctccgggtcc
     7741 ggacgcggcc ggccgggtcg cacggatggt cgttgacggc ggcgcccatc cggacgctgc
     7801 ccatctggtg ggcggagaag accgagccac ggttcgcgcc gaagtcgaac gaccggagcc
     7861 catcctcgaa gacgacgaac gagcgttcct cctggccggg ggcgaacccg ctccggccgt
     7921 gccaccgcgg cggcgtcccg acagccacga tctggcgcgc tccggcggcc cggacgatcc
     7981 cggccatcga caccattgcg tggcggagcg tcgcgacgcc gcgttcgtcg agggcatagt
     8041 cgatccgggc ccggccgctg ccggcgaggc ggacgcggcc cgcgccgccg tcgcgggtga
     8101 cggcgatcag cggcgcgacg tggcggatcc ggccgagcag gtcggcatgg gcgtcggttc
     8161 cttcccaggg cagggcgaga gcgatgagac cgggatggcc cggcgcggac tcgatgacgt
     8221 agccgttccg ggcgccggcc ggccggctga actccaggga ccgagccgcc tgcatcgtcc
     8281 cgcgccacat ctcgatcgga tcgtcgaaca ggccggcgac gacgggcgcc gggtggaggc
     8341 ggaggtggcg gccgatcgcc ggatgatcca ggccgctggc gagcagcacg gcgggcgagc
     8401 gcagcgcgcc cgcggcgacc acgacctggg cggtccgcac gaccaggcgt cgggtccgcg
     8461 gccgcagcgg atcgccgccg ggacccagcg ccggctcgcc cgttgccggg tcgacgacca
     8521 ggacgtcggc ctcgacaccg acaacccggc cgtgcacgat caggacgcgc gtcacccggg
     8581 ctcgatcgac gactcgcgcg cctgcagcga cggccgtcgc gaggtgtgct cgcatcccgg
     8641 actgcttggc gccgcgcgga cagccgaacg ggcagctccc gcaatcgccg caatcggtgg
     8701 cattgcgccg gatcggcgcc gcctcccagc cgaggttggc cgcgccacgg aggatgacgg
     8761 cgtccttcgg cgggatggcc gtcgccggcg cgacgccgag ctcgacctcg atcgtttgga
     8821 ggtcggcgtc gaactcggcg ccgtcgatcc cgtcgatccc gtggtcgctg gcccactccg
     8881 cccggacgtc gtccggcggc gcgatgctcg tcatccagtt gacgagcgtg ccaccgccga
     8941 ccgcggtccc ggcgagcatc gtgatcgccc cgtcccacgt cgtgagcagc ccatggttca
     9001 ggtagacccg ggagtaggcg tcgagctcgt cggtcgggag gtgggcctcg tcgaggaagg
     9061 ggcccgcctc gacgacgacg accgatcgac cggctcgggc gagctcggcc gcgatcaccc
     9121 cgccgcccgc cccggagccc acgacgacga cgtccgcgtc gagttgcacc gcgtcgtccg
     9181 ctccggtcgg ggggccgtgg atgtccagcg gccggatcgc ggcgagcagg gccgtgaccg
     9241 gcggacgctc ggggatgtag ccgatcgcgg cgtgaagggg attggcgatg cccggcggac
     9301 ccggatcggc gtaggcgagg aaggtgagga gcttgcggaa cgcctggtag gcggagcggc
     9361 ggagcgggag ccgcgagttc gcccaggcaa ggaggacccg ctcgcgatcg gccggcgtgc
     9421 ggtcccggaa cgcgatcggc cggccggtca ggagcaggtt ggccaggcgc gactcgaaca
     9481 ggcggaggac gagccggagc tggcggacct gggcggggtc ggcggcccgg gccagggcct
     9541 cggcggcgag attggaccgc cggaccgagc cgccccggac gaatgtctcg gccagctcgg
     9601 cgagggtcgc gaggtcggcg acggaccagc cgggcgggat ggtcggtggc tggctcgaca
     9661 tcgacggatc gtgccacatg cacgaggacc ggatcggtcg gcatcgcgcc gttgccgatc
     9721 ggtggagaac cgtcgcgggc tgagaccgcc ggtcgtccga ccgccgccgg tcagacggtc
     9781 cggcgccgcc gatggccgag gatcgcggca gcgagcaggg accccgccgc cgcggccagg
     9841 acgaccccga acccggcggc ccgcgggttg ccgttcagca gcccgcccac gacgacgtgc
     9901 cacagcccga acgccgtcgc agcgatgagg acgacgatga cgaccgcgaa gccgaccagg
     9961 cgcaggtccc ggaccaggat cacgcccgcc cggcgacggc gccagcacca gcgcccggac
    10021 gctcccagcc ctgggccggc agcggctcac agcggagcgg cctgtccgac cggtagccga
    10081 gggcgtactc gccctggatg atcgtgtgga gctggctcgt cccctcgtag atgacggccg
    10141 ccttggagtt tcgcaggtag cgctcgaccg ggaactcgtt cgaatagccg ttcgcgccgt
    10201 ggacctggat cgcgtcgagc gcggactgga ccgcgtgctc tgtggcgtgc cacttggcca
    10261 gcgatgtctc gcgggtgttg cggagaccct ggttcttcag ccacgccgcc cgccagacga
    10321 ggagccggcc catctccgtg ccggcggcca tcttcgcgat cagctgctgg accaactgat
    10381 ggcgaccgat ctcctgaccg aaggtggagc gctcgtgggc gtacttgagc gatgcgtcga
    10441 gacatgcctg ggcgagcccg acggcacccg cggcgacggt gaaccggccc tggtcgatcg
    10501 cgctcatcgc gatcaggaac ccctcgccct cctcgccgat ccggtggtcg gccggcacga
    10561 cgcaatcgtc gagattgatc aatcccgtgt tgccggcccg gatcccgagc ttgccgtgga
    10621 gcgttccggt cgtgaggccg gccattcccc gctcgaggag gaaggccgtc accccgcgcc
    10681 agcccttcga gcggtcgacc gaggcgaaca cgaggaagtg gtccgccagg tcggccaggc
    10741 tgatccagat cttctggccg ttgagccggt acgcggcccc gtcacggcgg gcggtcgtcg
    10801 cgaggttcgc cgcgtccgtc ccgacgcccg gttcggtcag cccgaacgtc gccagctttt
    10861 cgcccctcgc ctggggcgcg agccaacgct ccttctgctc gtccgtcgcc cactggagga
    10921 gggccagcga gttcagcccg acgtggacgc tctggacgac ccggaatgcc gtgtcggccc
    10981 gctccagctc ctcgcacagg atcgcgaagc tgaggtagtc catcccggac ccgccccacg
    11041 cctcggggat cggcgcgccg aggaagccct gctcggccat cttcgcgaag agctcgcgat
    11101 ggacctcgcc cttctcgtcc cactcgcgga tatggggcag gatctcggcc tcggcgaagg
    11161 cccgcgccgc ctgctggacg aggcggttct catcggtgag ctgaaagtcg accatcgccg
    11221 aatgctagcc gctcggcgcg tcaccggcgg cgcgtctctc gggcgtcgcg atcggcgacc
    11281 gccgccggcc gcgtccgatc gcggcgtcga gcccgacgag gtggtcgggc cgcggcgacg
    11341 gtgctcggcg tcgacgcacc gggccgaacg gcccagccgg ggccggagcc gcgctggcat
    11401 actcggtcga tgaccgacat cgccgttccc gccgccctcc cggccgatct ctcgaccgtc
    11461 gccgaccaca tcacccccgt cctcggccgc tacttcgagc ggacctggag ccacggtgag
    11521 ggccaccggc tgtacgactc gggcgggaag gcctacctcg acttcgcgaa cggcatcgct
    11581 gtcactgcgc tcggccatca ccaccccacg gtgacggcgg cgatccatgc ccaggtcgac
    11641 cggctgatcg gcccgaccgg ggcgatgggc ttcgccgagc cggtcgtccg cctcgccgac
    11701 atgcttgccg agacgctgcc ggccccgatc gac
//
LOCUS       DATAIW010000014         8235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149342, whole genome shotgun sequence.
ACCESSION   DATAIW010000014 DATAIW010000000
VERSION     DATAIW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149342"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..607)
                     /locus_tag="VHM48_00485"
     CDS             complement(380..607)
                     /locus_tag="VHM48_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753905.1"
                     /translation="MQQMYHPTDLASMDPLVLMKNLDHVRMTSRRLSYILQQQVHLYT
                     PEANQIREQIDRYVEAERQIEGEMARRRIRA"
     gene            complement(757..2154)
                     /locus_tag="VHM48_00490"
     CDS             complement(757..2154)
                     /locus_tag="VHM48_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX2753906.1"
                     /translation="MTIGPRPTAAQLALDLEVPAALPVLPIAPEWKDQARLWGPALHP
                     MCSYLASFPAALAHAFIGRYSRRGDVVLDPFSGRGTTALQACAEGRIGVGNDLNPLAH
                     LLTAAKVEPAAPAEAATRLATLRLGWAASSAEWLALADEVLADTADPPTVGIPVAGSR
                     NAAARAETVPKEVAVAFHRRTLGQLLYVRTVLRQDDRTDRFLAAALTGILHGKSASYL
                     SELMPNTFSMAPRYVRDFAARTAFASPDRDVFGGLEKKLARLHREALPRTPGIALLGN
                     ARDSAGRARTALRERGLPERVRLVVTSPPYLRVVKYGYYNWLRTWFLGEDARAIDAAL
                     DDAHHREPYLVFLREVLADLRTVLADDAVVVLVIGDVETDRGRPIRGAIGLAERVWER
                     AAEPEGYRLAGIALDDVAAGRKMTKLWGAEAGQATKTDRILVLGATEAGRRRALSAAR
                     TPIDWDWPPRGLRAI"
     gene            complement(2285..4378)
                     /locus_tag="VHM48_00495"
     CDS             complement(2285..4378)
                     /locus_tag="VHM48_00495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX2753907.1"
                     /translation="MTTLRLPLPASRPRRRPWADNRTLRRLSADPQALLALGLVATSM
                     AVAAIGGDGDQSRLLAISIEFLAAQAIAAVMAPHLRPTASQRTMIAFGRFALAILYVS
                     VVTALLRTGEFRPTGALYIPVVALAAAQGTRQALLVGSAAIALYLLPVLAATPDNLTL
                     DAQRAVALGGTAILLSIGTRRSISALTVTVRRLGASLARDRRRSRQVAAVESVGRLLA
                     STGPAPETLARIVGLLRDDLGYDFVSIYLGSAARVWIAAQAGYDTVIDEFDGSAGVIG
                     RVMRTNALAFVPDVAADADYLSAADVVMAEISAPLVVDDELVGIINVEARAPADLDAS
                     DVGTMRLVAERMASALALAGERERLAARAELFRRLTSFATAVNATLEPDRVQQAIVDE
                     LADVLAADSVGLTVLDRPTGRYVIRAVAGRDQQYLGLPIEPGSSLAGRAIRDRALVVD
                     HRYDPTAHPAAIAAGTPTEPMAGVAVPLIRDDVVVGALSLVRHDLDRPFTPDELEALP
                     ILAGVVALVITNTFLHADVIELSVRDALTGLFNRRHLDATIARLEAVRARRPGGTRDR
                     AAVAIFDLDHFGEINKRHGHQAGDAILRAFADILRGRFRGADLVARYGGEEFLAILDG
                     ATVEQAMAVAEEIRMAFATVAVTSPDGSPITATVSAGCAAMATDEERFDDVMARADVG
                     LVLAKRSGRNRVVAA"
     gene            4879..4954
                     /locus_tag="VHM48_00500"
     tRNA            4879..4954
                     /locus_tag="VHM48_00500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4912..4914,aa:Arg,seq:tct)
     gene            complement(4999..5292)
                     /locus_tag="VHM48_00505"
     CDS             complement(4999..5292)
                     /locus_tag="VHM48_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX2753908.1"
                     /translation="MSTCPHLWESREVAPASEGCAACIASGDTWHHLRLCLTCGNVGC
                     CDQSLNKHASAHFAETGHPVARSKQPGEAWRWCYADAVLDDPDELEMEGADLG"
     gene            complement(5355..6179)
                     /locus_tag="VHM48_00510"
     CDS             complement(5355..6179)
                     /locus_tag="VHM48_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2753909.1"
                     /translation="MAIKLGIIVTTGEPRVAAELAALAEARGWDGVFTWDGVAIGTMD
                     TFDPWVVMAAMAMRTERVTLGAIVTPPARRRPWKLAREAMTLDRLSNGRLVLPVGLGT
                     TDDAAFGNVGEPTEARIRAEKLDESLEILCGLWTGEPFGFAGRHYRFEPMTFRPTPVQ
                     QPRIPIWTVGAWPNERSMRRTLHWDGVVAQVEGAAAIREIVDWAEREWPAATRDRAWD
                     VVAEGNTPADDPAAGVATVTEHAAAGATWWIESDWESGTVETIRRRIEAGPSRPRG"
     gene            complement(6231..7763)
                     /locus_tag="VHM48_00515"
     CDS             complement(6231..7763)
                     /locus_tag="VHM48_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753910.1"
                     /translation="MTNLIDLDRALAEFLEDGPNTAPEAPVIAALAHARTTPRRPDPL
                     RWLRSDVMASTGRSSLVMRPGLVLGLLALLALAIGAAVIGGRQPTDPGLVVPSPSGPA
                     PTQAQPSAAPTTEPSGSAPPAPFSAQVPILVSAGQPFDITVTDVTGELIEAHSVQPGD
                     GATVGPTDIRVVADPADSAVFLVTWQGTPCEIGGSLHVDEPGKIIEVGRQRCLGDSLP
                     IDRIVRLRFRSAMSEGEWSSTFAEWPPAGGPEPSGSAAPLEPLGSPAVAPIHVALAHG
                     SGNAVSIDVVDESGLLVKAMSGTVPDTEPAHQFDISNDDPTTLRLTWEGSPCDTVHRL
                     TIDPTATGFTIDRPLCGGDAMAAWRSLVLTFSRPVSAATLSTSLVAGRGGVDMPNWTA
                     AAPDSADKRYDLTLSDPGYVVNTIEGYYDPSTTAASVGAAGIRLRATDDNTFTVIWLG
                     PACATTPTLAISPDGNAWRLVNEPCATSAANVVRMIDVGINLPRTTATVPSIVEVTAV
                     AP"
     gene            complement(7760..>8235)
                     /locus_tag="VHM48_00520"
     CDS             complement(7760..>8235)
                     /locus_tag="VHM48_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2753911.1"
                     /translation="YGIAYRILRDQYLAEDALQQALISIWNDLPKLRDPDRFDAWTYR
                     VIVRASTAEGRRAGHGHPTAPLLPADADVSRAPDQYRAVADRDQLERGFSRITPEQRA
                     VLVLQHYAGLSQAEIADILGVPIGTAGSRIHYAARALRAAIEADTRPGAGRESLA"
BASE COUNT         1165 a         2831 c         3092 g         1147 t
ORIGIN      
        1 gcgcgggctt cagcgtgcgt ggctcggtcg caactccgac gatgccgagc gcgctcaccg
       61 ctgatctgac tcagccactg agtcagggag aacgccgcga ccggccgcaa aggcgcgacg
      121 gccatcgacc ctgttgggag ataacctcag ttcgtcgcgg gcggggcgag gttcggcgtg
      181 gaggcgccgg tagcccgtgg cggctaacct aactgggcgc cggtacgacg agccattctg
      241 ccgccgttcg gggcaagtgg tcagggcacg aatatctggc cgagtcgagg gcgccttgag
      301 ccgtctcgat ccccttggcc cgaccggggc gagtacctgg ccggtcaggg gtgtgcgtgg
      361 gccatgcgcc gcgggccggt caggcgcgga tccggcgcct cgccatctcg ccctcgatct
      421 ggcgttccgc ctcgacgtag cggtcgatct gctcgcggat ctggttcgcc tcgggggtgt
      481 agaggtggac ctgctgctgg aggatgtacg acaggcggcg gctcgtcatc cggacgtgat
      541 ccaggttctt catcaggacc aggggatcca tcgaggcgag gtccgtcggg tggtacatct
      601 gctgcatggc cgcattctag ccgcgccgcc gtcgcgcgcg acgaccgagc gaccggctcc
      661 aggcgagctc ccatcggctg ccagcggcgg cgctgacgtc cttccagccc gccgggaggt
      721 tggccgggat gatgacggcc gacggcgcgg cgtccttcag atcgcgcgca acccgcgcgg
      781 cggccagtcc cagtcgatcg gcgttcgggc ggcggacagg gcgcgacgcc ggccggcctc
      841 cgtcgcgccg aggacgagga tccggtcggt cttcgtcgcc tggccggcct cggctcccca
      901 cagcttggtc atcttccggc cagccgcgac gtcatcgagg gcgatcccgg cgagccggta
      961 cccctcgggc tcggccgccc gctcccagac ccgctccgcc agcccgatcg cgcctcggat
     1021 cggccgtccg cgatcggtct cgacgtcgcc gatcaccagc acgaccacgg catcgtcggc
     1081 gaggacggtg cggaggtcgg ccagcacttc gcgaaggaag acgagatagg gctcgcggtg
     1141 gtgggcgtcg tcgagcgcgg cgtcgatcgc ccgggcgtcc tcgccgagga accacgtccg
     1201 gagccagttg tagtagccgt acttgacgac ccgaaggtag ggcggcgacg tgacgacgag
     1261 ccgcacccgc tcgggcaggc cgcgttcgcg gagggccgtt cgcgcccgcc cggcactgtc
     1321 ccgggcgttg ccgaggaggg cgatccccgg cgtccggggg agcgcctcgc ggtggaggcg
     1381 ggcaagcttc ttctcgagcc cgccgaacac gtcgcggtcg ggcgatgcga acgcggtccg
     1441 ggcggcgaaa tcccggacgt agcgcggcgc catgctgaag gtgttcggca tcagctcgga
     1501 gaggtacgag gcgctcttgc cgtggaggat cccggtcagg gcggcggcca ggaaccggtc
     1561 ggtccggtcg tcctggcgga gcacggtccg gacgtacagc agctgcccga gcgtgcggcg
     1621 gtggaaggcc accgcgacct ccttcgggac cgtctcggcg cgggccgcgg cattccggct
     1681 gccggccacg ggaatgccga cggtcggcgg gtcggcggtg tccgcgagga cctcgtcggc
     1741 gagagccagc cactccgcgg acgatgctgc ccagccgaga cggagggtcg caaggcgggt
     1801 cgcggcctcg gccggcgcgg ccggttcgac cttggccgcc gtcaggaggt gggcgagcgg
     1861 gttgaggtcg ttgccgacgc cgatccggcc ctcggcgcag gcctgcaggg cggtcgtgcc
     1921 gcggcccgag aacgggtcga ggacgacatc gccgcgccgc gagtagcgcc cgatgaaggc
     1981 gtgggcgaga gccgccggga agctcgccag gtaggaacac atcgggtgga gggcggggcc
     2041 ccagagccgc gcctggtcct tccactccgg ggcgatcggg aggaccggca gtgctgccgg
     2101 gacctcgagg tcgagcgcga gctgggcggc cgtcggccgc gggccgatcg tcatcctggg
     2161 ctggtgctcc tgctggctga cgggccgcgg agcataccag accggattcg ccggttcggt
     2221 cgggtcttgc gccggttggg aacgaggaat ccgacgtgga acggtcgccg ttgcctcgcc
     2281 ccggtcaggc cgcgacgacc cggttgcgac cggatcgctt ggcgaggacc agtcccacat
     2341 cagcccgcgc catgacgtcg tcgaaccgct cctcgtcggt cgccatcgcc gcacagccgg
     2401 cggacacggt ggcggtgatc ggtgagccat cgggactcgt tacggcgacc gtggcgaacg
     2461 ccatcctgat ctcctcggcc acggccattg cttgctcgac cgtcgccccg tcgaggatcg
     2521 cgaggaactc ctcgccaccg tagcgggcaa cgaggtcggc gccgcggaac cgcccgcgga
     2581 ggatatcggc gaaggcgcgc aggatcgcgt cgccggcctg gtggccgtgg cgcttgttga
     2641 tctcgccgaa gtggtcgagg tcgaagatcg cgacggccgc gcggtcacgg gtcccgcccg
     2701 gccgtcgggc ccggacggcc tcgagccggg cgatggtcgc atcgagatgg cgacggttga
     2761 agaggccggt gagggcgtca cggacggaca gctcgatgac atcggcatgg aggaacgtgt
     2821 tcgtgatcac gagggcgacg acgccggcca ggatcggcag ggcctcgagc tcgtcaggtg
     2881 tgaacggtcg atcgaggtca tggcgcacga gggacagcgc gccgacgacg acgtcgtcgc
     2941 gaatcagcgg taccgcaacg cccgccatcg gctcggtcgg ggttccggcc gcgatggcgg
     3001 ccggatgggc cgtcgggtcg tagcgatgat cgacgacgag tgcccggtcg cggatggctc
     3061 gcccggcaag gctgctgcca ggctcgatcg gcaggccgag gtactgctgg tcgcggcccg
     3121 ccaccgcccg gatgacgtac cggcccgtcg ggcgatcgag gaccgtcagg ccgaccgaat
     3181 ccgcggccag gacatcggcc agctcatcga cgatcgcctg ctggacgcgg tcgggctcga
     3241 gcgtcgcatt gacggccgtg gcgaacgatg tcaggcgccg gaagagctcg gcacgggcgg
     3301 caagacgctc ccgttcgccg gcaagagcga gggccgacgc catgcgctcg gcgacgagtc
     3361 gcatcgtccc gacgtcgctc gcgtcgaggt cggcgggcgc ccgggcctcg acattgatga
     3421 tcccgaccag ctcgtcatcg acgaccagcg gcgcgctgat ctcggccatg acgacgtcgg
     3481 cggcgctgag gtagtcggcg tcggccgcga cgtccgggac gaacgcgagg gcattcgtgc
     3541 gcatcacccg cccgatcaca ccggcggagc cgtcgaactc gtcgatgacc gtgtcgtagc
     3601 cggcctgcgc ggcgatccag acgcgggctg cggagccgag gtagatcgag acgaagtcgt
     3661 aaccgagatc gtcgcgcagc aggcccacga tccgggcgag ggtctcgggg gcgggaccgg
     3721 tcgacgccag cagccggccg acgctctcga ccgccgcgac ctggcgggag cgccgtcgat
     3781 cacgggccag cgaggcgccc aggcgtcgga ccgtcaccgt cagcgccgag atcgatcggc
     3841 gtgttccgat cgacaggaga atcgccgttc cacccagggc gacggctcgc tgcgcgtcga
     3901 gggtcaggtt gtcgggcgtc gcggcgagga cgggcagcag gtacagggcg atcgcggccg
     3961 agccgaccag cagcgcctga cgggtgccct gggcggccgc cagcgcgacg accgggatgt
     4021 acagggctcc ggtcggacgg aactcgccgg tccggagcag ggccgtgacg acgctcacgt
     4081 acaggatcgc gagggcgaag cggccgaacg cgatcatcgt ccgctggctc gccgtcggcc
     4141 gaaggtgcgg tgccatgacg gccgcgatgg cttgcgcggc gaggaactcg atgctgatcg
     4201 cgagcaggcg cgattgatcg ccgtcgccgc cgatcgcggc gaccgccatc gacgtggcga
     4261 ccagcccgag cgcgaggagc gcctgggggt cggccgaaag acgccgcagg gtccggttgt
     4321 cggcccacgg ccgtcgtcgc gggcgcgacg cgggcagggg caggcgcagg gtcgtcatcg
     4381 cgccaaccgt agcggtgcgt ccgcgcgccg gtcaccgtga cagtggtccc gacgggactg
     4441 gtccgttcgc cgtaggactt gccggtcgcg gcggaacgac cagggcggcg ttggccgctg
     4501 cgttcctcgc tcacggccat cggaggccgc tcactccggt gctcggggcc gccttgccct
     4561 ggccgcccct ccgcgacacg tgggtcggag cgcggcggag acgaccaggg cggcgttcag
     4621 ccgccgatca gccggagatc gaccccacct tgacgacggt ctcgctcgag gtctcgtcga
     4681 gcttgaacga gtcgttcacg tcgtagatca cgcgcttccg cttctcgtcc ttctccacga
     4741 accggatcgg ccgggtgagt ccacgacggt ttctgggtgt ccacacctgc ccttggggag
     4801 cgtcatggca accgcgaacc caccaggtgc cgaacgatcg atggggtaga ttcgtgcgag
     4861 aaccaagcag ctgaccgcgt ccccgtagct cagtggacag agcaaccgcc ttctaagcgg
     4921 aaggtcgcag gttcgaaccc tgccggggac gccatcctct cgcccgcaac gattgcgcga
     4981 cgctcgcggg caacgagttc agccgaggtc cgcgccctcc atctcgagct cgtcgggatc
     5041 gtcgaggacc gcgtccgcgt agcaccagcg ccaggcctcg cccggttgct tggagcgcgc
     5101 caccggatgc ccggtctcgg cgaagtgggc cgacgcgtgc ttgttgaggc tctggtcaca
     5161 acagccgacg ttgccgcacg tcaagcagag ccggaggtgg tgccacgtgt cgccgctcgc
     5221 gatgcacgcc gcgcaaccct cagacgcggg cgccacctcg cgcgactccc agaggtgggg
     5281 gcaggtactc atcgcgcggg agcgtacacc tgcgggacgg gcgcgatcag gatgccgacc
     5341 tggtcctgga cccgtcagcc ccgtggtcgc gacggtccgg cctcgatccg ccgccggatc
     5401 gtctcgaccg tgccggactc ccagtccgac tcgatccacc aggtcgcgcc cgcggcggcg
     5461 tgctccgtga ccgtcgcgac gcctgcggcc gggtcgtccg cgggcgtgtt cccctccgcg
     5521 accacgtccc aggcacgatc gcgggtcgcg gccggccatt cgcgctcggc ccagtcgacg
     5581 atctcccgga tcgcggcggc gccctcgacc tgggcgacga ccccgtccca gtgcagggtc
     5641 cgtcgcatcg accgctcgtt cggccaggca ccgaccgtcc agatcgggat ccgcggctgc
     5701 tggacggggg tcggccggaa ggtcatcggc tcgaagcggt agtggcggcc ggcgaagccg
     5761 aatggctcgc ccgtccacag accgcacagg atctcgaggc tctcgtcgag cttctcggcc
     5821 cggatccggg cttccgtcgg ctcgccgacg ttgccgaagg ccgcgtcgtc ggtcgtcccg
     5881 agcccgacgg gcaggaccag ccggccgttc gacagccgat cgagcgtcat cgcctcgcgg
     5941 gcgagcttcc acggccggcg gcgggccggc ggcgtgacga tcgccccgag cgtgacccgc
     6001 tcggtccgca tcgccatcgc ggccatgacg acccacgggt cgaatgtgtc catcgtcccg
     6061 atcgcgacgc cgtcccaggt gaagacgccg tcccagcccc gcgcttcggc gagcgccgcg
     6121 agctccgccg caacccgcgg ctcaccggtg gtgacgatga tcccgagctt gatggccacg
     6181 ccccgatggt aggggaggcg accccggggc gcgtcaggag gccggcgggc tcacggcgcg
     6241 accgcggtca cctccacgat cgacgggacc gtcgcggtcg tccgcggcag gttgatgccg
     6301 acgtcgatca tccggaccac gttggcggcg ctcgtcgcgc atggctcgtt gacgagccgc
     6361 caggcgttcc cgtccgggct gatcgcaagc gtcggggtcg tcgcgcacgc cggaccgagc
     6421 cagatcaccg tgaacgtatt gtcgtcggtc gcgcggaggc ggatccccgc ggcgccaacg
     6481 ctggcggcgg tcgtggacgg gtcgtaatag ccctcgatcg tgttcacgac gtagcccgga
     6541 tcggacaggg tcaggtcgta gcgcttgtcg gccgagtccg gtgccgcggc cgtccagttc
     6601 ggcatgtcca ccccgccgcg gccggcgacg agcgatgtgg agagtgtcgc cgcggagact
     6661 ggccggctga acgtcaggac gagcgatcgc caggccgcca tcgcatcgcc cccgcagagg
     6721 ggccggtcga tcgtgaagcc cgtcgccgtc ggatcgatcg tcaggcggtg gaccgtatcg
     6781 cacggcgatc cctcccacgt caggcggagg gtcgtcgggt cgtcgttgga gatgtcgaac
     6841 tggtgcgcgg gctccgtgtc cggcacggtc ccggacatcg ccttgacgag caggccggac
     6901 tcgtcgacga cgtcgatcga gaccgcgttg ccgctaccgt gggccagggc gacgtggatc
     6961 ggggcgaccg cgggcgaacc gagcggctcg agcggtgccg ccgagcccga gggttcggga
     7021 ccgcccgctg gcggccactc cgcgaacgtc gaggaccatt cgccttcgga catcgccgat
     7081 cggaaccgga gacggacgat ccggtcgatc ggcagggaat cgcccaagca gcgctggcgg
     7141 ccgacctcga tgatcttccc gggttcgtcc acgtggagcg agccgccgat ctcacacggc
     7201 gttccctgcc acgtcacgag gaacaccgca gagtcggccg gatccgcgac cacgcggatg
     7261 tccgtcggcc cgaccgtggc cccgtcgccg ggctgtacgg aatgggcttc gatgagctct
     7321 cccgtcacgt ctgtgaccgt gatgtcgaac ggctgccccg cactcaccag gatcgggacc
     7381 tgggccgaga acggcgcggg cggagccgat cccgacggct cggtggtcgg cgcagccgac
     7441 ggctgggctt gggtcggcgc gggccccgat ggtgagggga cgaccagccc cggatccgtc
     7501 ggctggcgcc cgccgatgac cgcggccccg attgccaggg cgagcagggc gagcaggccg
     7561 agcacgaggc ccggccgcat gaccagcgac gatcgaccgg tggaggccat cacgtcggac
     7621 cgcagccagc ggagcgggtc cgggcggcgc ggcgtcgtcc gggcgtgggc gagggcggcg
     7681 atgaccggcg cctcgggagc ggtgttcgga ccgtcctcga gaaactccgc cagggcgcga
     7741 tcgaggtcga tgaggttcgt catgcgaggg actcccgccc ggctcccggc cgggtatcgg
     7801 cttcgatcgc ggcccgcagg gcgcgggcgg cgtagtggat ccgcgagccg gccgtcccga
     7861 tggggacccc gaggatgtcg gcgatctcgg cctgactgag gccggcgtag tgctgcagga
     7921 cgaggaccgc ccgctgctcg ggcgtgatcc ggctgaagcc ccgttcgagc tggtcgcgat
     7981 cggcgacggc gcgatactgg tcgggggcac gcgacacgtc ggcatcggcc ggcagcaagg
     8041 gcgccgtcgg gtggccgtgg ccggctcggc ggccctcggc ggtcgatgcc cgcacgatca
     8101 cgcgatacgt ccaggcgtcg aagcggtccg ggtcgcggag cttcggcagg tcgttccaga
     8161 tgctgatcag ggcctgctgg agggcgtcct cggcgaggta ctggtcgcgc aggatccggt
     8221 aggcgatccc gtaga
//
LOCUS       DATAIW010000015         8628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149346, whole genome shotgun sequence.
ACCESSION   DATAIW010000015 DATAIW010000000
VERSION     DATAIW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149346"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VHM48_00525"
     CDS             <1..1433
                     /locus_tag="VHM48_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2753912.1"
                     /translation="AGFAAAAATLPPLGRDVVFVLLLVAFGTKAGAIPLHIWLPRAHP
                     VAPSHVSALMSGVMIKAGIYGIIRLGIEVLGRGPEWWGLLVLAIGVTSAVLGVLYALM
                     EHDLKRLLAFHSIENVGIILIGVGIALLAVSNDAAQLARLALIAALFHTLNHALFKGA
                     LFLGAGSIQAAVGTRDLNALGGLARVMPITTLAFGIAAAAISGLPPLNGFASEWLTFQ
                     ALIGAGGESGLSPIARSAALLAVGGLALTAALAVACFVKATGMAFLALPRSAAAAAAH
                     EVNRTMRSAAAVLAAACVAVGLAAGPVAVVLAGVAGGVIVANPSGAANGALVAVQAIP
                     GGAAYAAGPLGLLLVALAAGTWFVAGRGRTTRRTATWTCGIVPEPAFEYNATSFAKLI
                     RLYFGPILRPAREVTVELHPGTPFPRTVRYRGEVTHLLDERLYGPLHRAAVTAAQLIR
                     RLQSGSLQLYLAYAVAAVIGLLLLAR"
     gene            1433..2395
                     /locus_tag="VHM48_00530"
     CDS             1433..2395
                     /locus_tag="VHM48_00530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX2753913.1"
                     /translation="MAELTTFQAVVLGLVQVGLVLLIAPLLLGVTKRVKARLQYRRGP
                     SLLQPYADLAKWWRKEVVESDAASPLTAAAPAIGFAALLLATFLVPIVGQRPPLDGWG
                     DLLVVVGLLALGRFVLALAALDSGSAFGGMGSSREVAIAALVEPALVLALAGAAVAAG
                     STDLGVISRTATGAGFAWYVPAVLLAAAAYALVAVAETGHEPVDNPDTHLELTMIHEG
                     MLLEASGPRLAILAYTAELKLVLVAALFAAAFAPAGAAGEVAPIPLLVGLAAAILKLV
                     AAAVVLGVLDATLPKLRILALPGLLATASILAAVGLAARIWLVA"
     gene            2392..3057
                     /locus_tag="VHM48_00535"
     CDS             2392..3057
                     /locus_tag="VHM48_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753914.1"
                     /translation="MTLDPAAAASIIDACAAAIVLVGVGLAATRSIGRSIWLVAIQAI
                     LAGVAAIGVGLATGVGAVVTGGLLAIVIKGAVVPVVLGSILRRSAVRRERHPILGPRA
                     SLIVAVVIVFAANAAASGVTLGAGVAATRALPAAIAQVLTGLLIVMTRRKVLSLVVGL
                     LVFENGIALTAFALTYGMPLVVELGILFDVLIAVIVAWVYAGRMIDVFGTTSTDQLRN
                     LRG"
     gene            3054..4598
                     /locus_tag="VHM48_00540"
     CDS             3054..4598
                     /locus_tag="VHM48_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2753915.1"
                     /translation="MIELLVSLVVLAPLGGGAAAWVSDDRRAVRLAVGGLGVSIAAGL
                     ALAGIVLGSGPVRAFDGLVVVDALGAAILVLVLVVAAIALAGSPRHLDHEAAAARLRP
                     HDRGRYYALLLWFAGGLATIPLVDSLGLVWVGIEATTIVSALLVGFARTPTAIEAAWK
                     YLILGSIGIGFALLGTLLLYASSVGVLGETSDALAWTRLIAIAPRLDPGLARLAFIFA
                     LAGYGTKAGLVPFHTWLPDAHSQAPSPISALLSGAALAVALYALARFHLVVVGTLGPA
                     FSSTLLVLFGLLSLAIALPFIVAQGDLKRLLAYSSIEHIGIATLALGFGGRVALLGLT
                     LHLASHGLAKATAFLAAGQLVAERGSRRIGRLAGSLARSPADGRALVVASLALAGLPP
                     SGIFVGELAIIFGGVQAGWGAAAAAAALILGLAVAGLLFHIVRVAVGPAGSPTGGSRS
                     SGPSTVALAQPPGATTSAPSAPSRGWAVVLATPLAVVVIAGLWTPSPLGAALDRIVDV
                     LAGGHG"
     gene            4573..6135
                     /locus_tag="VHM48_00545"
     CDS             4573..6135
                     /locus_tag="VHM48_00545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2753916.1"
                     /translation="MSWPAVMAERPENARHRIGDGVVELVDPEALGPRVADLVAAGGR
                     HVMLLGLDERSVGGNGLAVEAVMLTTDGSLNRVRAALAPDEPAYPSISSIMPAASWDE
                     RELKDLFGILPHGHPDPRRLVLHERWPRGHHPLRKDVAAGDRPPEADRHFVPFEVHGE
                     GVYQLPVGPIHAGVIEPGHFRFSAVGERILHLDARLGFVHRGLEKIVEGRSFAAAAPV
                     VERACGVCTVTHGLAYAEAVEQLTATGIPERARRLRVLLAELERLYNHVGDLGNVCAG
                     IGFQPAVARLGWLKEQLFQLNDGLTGHRYLTGIVAPGGLWADVDAAALAGLPARLAAI
                     GTELAAAIRAILRSEGVMARFHGTGIVPQQTALALGALGVAARASGVGLDLRRDRPSG
                     GYRDLEVRPVTASGGDVAARFHVRAQEAHESLRIMREVLDRLPPGDISVPLAATPAVG
                     ETALGAAEGPRGASWIWLRAGNDGTIDRLHLRSASFANWPVVAAAVPGNLVPDFPLIN
                     KSFELCYACTDR"
     gene            6138..6482
                     /locus_tag="VHM48_00550"
     CDS             6138..6482
                     /locus_tag="VHM48_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2753917.1"
                     /translation="MPFDVFDRLLRPLRRGPVTNRYPDVPLEPPGATRGLPVLDGSRC
                     DGTAACVEICPTDAIRLSETTWTLDVGRCIFCGACARVCPRDAIRLGSTVELAVRDRD
                     DLVIVTQREARP"
     gene            6479..7000
                     /locus_tag="VHM48_00555"
     CDS             6479..7000
                     /locus_tag="VHM48_00555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX2753918.1"
                     /translation="MTDELIEPVASMPRLPAVVAATEAGRLRRAIRKAFGRSLHVRHL
                     DAGSCNGCDWEIAALLNPYHDVQRLGIDFVASPRHADLLLVTGIMTRNLEAAARRTYE
                     AMPRPRLVVAVGACAISGGVFAGTYAGRDGIGEVLPVDVFVAGCPPRPEALIHGLLTA
                     VGRMAPFRPEGAE"
     gene            complement(7336..7650)
                     /locus_tag="VHM48_00560"
     CDS             complement(7336..7650)
                     /locus_tag="VHM48_00560"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX2753919.1"
                     /translation="MASPTSIDDYLASLPDDRRAAVDLLRQTIKVAAPDATETIAYSM
                     PAFRSHGGQFLVSYAAYKRHCSLFPASGAVVEALGEELAPSSPARGRSSSRSIGRSRL
                     RS"
     gene            complement(7665..8105)
                     /locus_tag="VHM48_00565"
     CDS             complement(7665..8105)
                     /locus_tag="VHM48_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2753920.1"
                     /translation="MRISRVCFVGVRTENFDAMTGFVRDVLGLPSAHLDAGWGVFSLE
                     SGDRDFVEVYRPGQYDERLLPAAAHGPTVSFAVDDLLAARAELLAAGIEIVADVVWAA
                     DLFGSSQDPGYGWLFFRAPDGNVYVLQQEQAPVDPAPSIDVDAR"
BASE COUNT         1024 a         3246 c         3001 g         1357 t
ORIGIN      
        1 tcgccgggtt cgcggccgcg gccgccacgc tgccgccgct cggacgggat gtcgtcttcg
       61 tcctcctcct cgtcgccttc gggacgaagg ccggcgcgat cccgctgcac atttggctgc
      121 cgcgagccca ccccgtcgcg ccgtcgcacg tatcggccct gatgtccggc gtgatgatca
      181 aggccgggat ctacgggatc atccgcctcg ggatcgaggt cctgggtcgc gggccggagt
      241 ggtgggggct gctcgtcctc gccatcgggg tgacgtcggc cgtgctcggc gtcctctacg
      301 cgttgatgga gcacgacctc aagcgcctcc tcgcgttcca cagcatcgag aacgtcggga
      361 tcatcctcat cggcgtcggg atcgcgctcc tcgccgtgag caacgatgcg gcacagctcg
      421 cccgcctggc cctgatcgcc gcgctgttcc acacgctcaa ccacgcgctc ttcaagggcg
      481 ccctgttcct cggggcaggc tccatccagg cggcggtcgg gacccgggac ctcaacgccc
      541 tcggcggact cgcccgggtg atgccgatca cgacgctcgc cttcggcatc gccgcggcgg
      601 cgatcagcgg tttgccgccg ctcaacggct tcgcgagcga gtggctgacc ttccaggccc
      661 tcatcggcgc cggtggggag agcggcctct cgccgatcgc ccgatccgcc gccctcctcg
      721 cggtcggggg cctcgccctc accgccgcgc tcgcggtcgc ctgcttcgtc aaggcgacgg
      781 ggatggcgtt cctcgcgctg ccccggagcg ccgccgcggc cgctgcccac gaggtcaacc
      841 gcacgatgcg ctcggcggcg gccgtcctgg cagcagcgtg cgtcgccgtc ggcctggccg
      901 ccggacccgt ggccgtcgtc cttgccggcg tggcgggcgg cgtcatcgtg gcgaacccgt
      961 ccggcgccgc gaatggcgcg ctcgtcgccg tccaggcgat ccccggcggc gcggcgtatg
     1021 ccgccggacc gctcggcctc ctgctcgtgg cgctggccgc gggcacgtgg ttcgtggcgg
     1081 gacgcggtcg aacgacccgg cgaaccgcga cctggacctg cgggatcgtg ccggagcccg
     1141 ccttcgagta caacgcgacg agcttcgcga agctcatccg cctctacttc gggccgatcc
     1201 tgcggccggc gcgagaggtc accgtggagc tccatccggg aaccccgttc ccgcggacgg
     1261 tccgctaccg gggcgaggtg acccacctcc tcgatgagcg cctgtatggg ccgttgcatc
     1321 gcgcggccgt gaccgcggcc cagctgatcc gccgcctcca gagcggcagc ctgcagctgt
     1381 acctggccta cgccgtcgcc gcggtcatcg gcctcctcct gctcgcccgc tgatggccga
     1441 gctgaccacg ttccaggccg tggtcctcgg gctggtccag gtcgggctgg tcctgctcat
     1501 cgctccgctc ctgctcgggg tcacgaagcg ggtcaaggcc cgcctccagt atcgccgcgg
     1561 accgtcgctg ctccagccat acgcagacct ggcgaagtgg tggcggaagg aggtcgtcga
     1621 gagcgacgcc gcgagccccc tcaccgcggc cgctccggcg atcgggttcg ccgcgctcct
     1681 gctcgcgacg ttcctcgtgc cgatcgtcgg ccagcgtccg cccctcgacg gctggggcga
     1741 tctcctggtc gtcgtggggc tcctggcgct cggccggttc gtcctcgccc tggccgccct
     1801 cgattccggc agcgcgttcg ggggcatggg cagcagccgc gaggtggcga tcgccgccct
     1861 cgtcgagccg gcgctcgtcc tcgccctggc cggcgccgcg gtcgcggccg gctcgaccga
     1921 tctcggggtg atcagccgga ccgcgaccgg ggccgggttc gcctggtacg taccggcggt
     1981 cctgctggcg gcggctgcct acgcgctcgt ggccgttgcc gagaccggcc acgagcccgt
     2041 cgacaatccc gacacgcatc tcgagttgac gatgatccac gaggggatgc tgctcgaggc
     2101 gtccgggccg cggctcgcca tcctggccta caccgccgag ctcaagctgg tcctcgtcgc
     2161 ggcgctcttc gccgcggcct tcgcaccggc cggggcggct ggcgaggtcg ccccgatccc
     2221 gctgctcgtg ggcctcgcgg cggcgatcct caagctcgtc gccgctgcgg tcgtcctcgg
     2281 cgtcctcgat gcgaccctcc ccaagctccg catcctggcc ctccccgggc tgctggccac
     2341 ggcatccatc ctggccgcgg tcgggctcgc cgcgcgcatc tggctcgtcg catgacgctc
     2401 gacccggccg ccgccgcttc gatcatcgat gcctgcgcgg ccgccatcgt cctcgtggga
     2461 gtcggcctcg cggcgacccg ctcgatcggt cgatcgatct ggctcgtcgc gatccaggcg
     2521 atcctcgcgg gggtcgccgc gatcggcgtg ggcctcgcga ccggcgtcgg agcggtcgtg
     2581 accggcggcc tgctggcgat cgtcatcaag ggtgccgtgg tcccggtcgt cctcggctcg
     2641 atcctgcgtc ggtcggccgt gcgccgcgag cgccatccga tcctggggcc ccgcgcctcg
     2701 ctgatcgtgg cggtggtgat cgtcttcgcg gcgaacgctg ccgccagcgg ggtgacgctc
     2761 ggagccggcg tcgcggcgac ccgcgccctg ccggcagcga tcgcccaggt gctgacgggc
     2821 ctgctgatcg tcatgacccg gcgcaaggtg ctgtcgctcg tcgtcggcct gctcgtcttc
     2881 gagaacggga tcgcgctgac ggccttcgcc ctgacctacg ggatgccgct cgtggtggag
     2941 ctcgggatcc tgttcgacgt gctgatcgcg gtgatcgtcg cctgggtcta cgccgggcgg
     3001 atgatcgacg tcttcgggac gacgtcgacc gatcagctcc ggaacctgcg cggatgatcg
     3061 agctcctcgt ctccctcgtc gtcctggcgc ccctcggcgg cggggcggcg gcatgggtga
     3121 gcgacgatcg ccgcgccgtt cgcctggccg tcggcgggtt gggcgtgtcc atcgccgccg
     3181 ggctcgccct ggccggcatc gtcctgggct ccggccccgt ccgggcgttc gatggactcg
     3241 tcgtcgttga cgcccttggg gccgccatcc tcgtcctcgt cctggtcgtg gcggcgatcg
     3301 ccctggcggg ctcgccccgg cacctcgacc acgaggccgc cgccgcccgt cttcgtccac
     3361 atgaccgcgg tcgctactac gccctcctcc tgtggttcgc cggcggcctg gcgacgatcc
     3421 cgctggtcga cagcctcggg ctggtctggg tcgggatcga ggcgacgacg atcgtctcgg
     3481 ccctgctcgt cgggttcgcc cggacaccga ccgcgatcga ggccgcctgg aagtacctga
     3541 tcctcggttc gatcgggatc ggcttcgcgc tgctcgggac gctcctgctc tacgcgtcgt
     3601 ccgtcggcgt cctcggcgag acgagcgacg cgctggcgtg gacccggctg atcgcgatcg
     3661 ccccgcggct cgacccgggc ctcgcccgcc tcgccttcat cttcgccctg gccgggtatg
     3721 gcacgaaggc cggcctggtg cccttccaca cctggctgcc cgacgcccac agccaggcgc
     3781 cgagcccgat ctccgcgctc ctgtcggggg cagcgctcgc ggtggcgctc tatgccctcg
     3841 cccgcttcca cctcgtcgtc gtcggcacac tcgggccggc gttctcgtcg acgttgctcg
     3901 tcctgttcgg gctcctcagc ctcgccatcg cactgccgtt catcgtcgcc cagggcgacc
     3961 tgaagcggct cctggcgtat tcgtcgatcg agcacatcgg catcgccacc ctggccctcg
     4021 gcttcggggg tcgggtcgcg ctgctcgggc tgacgctcca cctggccagt cacggcctgg
     4081 cgaaggcgac cgcgttcctt gccgcgggcc agctcgtggc cgagcgcgga agccggcgga
     4141 tcggtcgcct ggccgggagc ctggcacgat cgcccgccga cggccgagcc ctggtcgtcg
     4201 cgagcctggc cctcgcgggg ctcccgccgt cggggatctt cgtcggggaa ctcgcgatca
     4261 tcttcggcgg ggtgcaggcc gggtggggcg cggcggccgc ggcggcggcg ctgatcctcg
     4321 gcctggcggt cgccggtctc ctgttccaca tcgtccgggt ggcggtcgga ccagccgggt
     4381 cgccgaccgg cggatcccga tcgtccgggc cgtcgaccgt ggcgctggcc cagccaccag
     4441 gcgcgacaac gagtgcgcca tcggcgccgt cccgcggctg ggcggtggtc ctggccacgc
     4501 cgctcgccgt cgtggtgatc gccggcctct ggaccccctc ccccctcgga gccgcgctcg
     4561 accggatcgt cgatgtcctg gccggcggtc atggctgagc ggcccgagaa cgcccgacac
     4621 cggatcggcg atggcgtcgt ggaactcgtc gacccggagg cgctcggtcc gcgcgtcgcc
     4681 gacctcgtcg ccgccggtgg ccgtcacgtg atgctgctcg ggctcgacga acgctccgtc
     4741 ggcggcaacg gcctggcggt ggaggccgtg atgctgacca ccgacggatc gctgaaccgc
     4801 gtccgcgcgg cgctcgcgcc ggacgagccg gcctacccgt cgatcagctc gatcatgccg
     4861 gccgcgtcct gggacgagcg cgagctgaag gacctcttcg ggatcctccc gcacggtcat
     4921 cccgacccgc gccggctcgt cctccacgaa cgctggccgc gcggccatca cccgctccgc
     4981 aaggacgtcg cggccggaga ccggccacct gaggccgacc gccatttcgt cccgttcgag
     5041 gtccacggcg aaggcgtcta ccagctcccc gtcgggccga tccacgccgg cgtcatcgaa
     5101 cccggccatt tccgcttctc ggccgtcggc gagcggatcc tccatctcga cgcccggctg
     5161 ggcttcgtcc accgcgggct cgagaagatc gttgaggggc gctcgttcgc ggcagcggcc
     5221 ccggtcgtcg agcgcgcctg cggcgtgtgc acggtgaccc acggcctcgc ctacgcggag
     5281 gcggtcgaac agctgaccgc gaccggcatt cccgagcgcg cccgccggct gcgcgtgctg
     5341 ctggccgagc tcgagcgtct ctacaaccac gtcggcgatc tcggcaacgt ctgcgccggg
     5401 atcgggttcc agcccgccgt cgcgcggttg ggctggctga aggagcagct gttccagctc
     5461 aacgacgggc tgacggggca ccgctacctg acggggatcg tggcccccgg cgggctctgg
     5521 gcggacgtcg atgccgccgc cctcgccggc cttccggcgc gtctcgccgc gatcggcacc
     5581 gagctcgccg ccgcaatccg cgcgatcctt cgttccgagg gcgtcatggc ccgcttccac
     5641 ggcaccggca tcgttccgca gcagaccgcg ctggcactcg gcgccctcgg cgtggccgcc
     5701 cgcgcctccg gggtcgggct cgaccttcga cgcgaccgac cgtccggcgg ctaccgcgac
     5761 ctcgaggtcc gtccggtcac ggcctccggc ggcgacgtcg cggcgcggtt ccacgtccgt
     5821 gcccaggagg cccacgagtc gctgcggatc atgcgcgagg tgctcgatcg cctgccgccg
     5881 ggcgacatct ccgtcccgct cgcggccacg ccggctgtcg gcgagacggc tctcggcgcg
     5941 gcggaagggc cgcgcggggc gtcgtggatc tggctgcgcg ccgggaacga cgggacgatc
     6001 gaccggctcc acctgcgatc ggcctcgttc gccaactggc cggtggtcgc ggccgcggtc
     6061 cccggcaacc tcgtcccgga cttccccctg atcaacaaga gcttcgagct ctgctacgcc
     6121 tgcacggacc gctgacgatg cccttcgatg tcttcgaccg cctgctccgg ccgcttcgcc
     6181 gcgggccggt cacgaaccgc taccccgacg tcccgctcga accgcccggg gcgacgcgcg
     6241 gcctgccggt gctcgacgga tcgcgctgcg acggcacggc tgcatgcgtc gagatctgtc
     6301 cgaccgacgc gatccggctc tcggagacga cgtggacgct cgacgtcggc cggtgcatct
     6361 tctgcggggc ctgcgcccgg gtctgcccgc gtgacgcgat ccggctcggg agcacggtcg
     6421 aactcgccgt gcgcgaccgc gacgacctgg tgatcgtcac ccaacgggag gccaggccgt
     6481 gaccgacgag ctcatcgaac cagtggcgtc gatgccgcgg ctgcccgcgg tcgtcgcggc
     6541 cacggaggcc gggcgcctcc gccgcgcgat ccgcaaggcg ttcggccgct cgctccacgt
     6601 ccgtcacctg gacgccggct cgtgcaacgg ctgcgactgg gagatcgccg ccctcctgaa
     6661 cccgtaccac gacgtccagc gtctcgggat cgacttcgtc gcctcgccac ggcatgccga
     6721 cctgctgctc gtgacgggga tcatgacccg caacctcgag gctgccgcgc gccggaccta
     6781 cgaggcgatg ccgagaccgc gcctcgtcgt cgcggtcgga gcgtgcgcga tcagcggcgg
     6841 cgtgttcgcc gggacgtacg ccggccgcga cggcatcggg gaggtgctgc cggtcgacgt
     6901 cttcgtggcg ggctgcccac cccgccccga ggcgctcatc cacggcctgc tgacggccgt
     6961 cggccggatg gccccattcc gccccgaggg ggccgaatag ctccgatcgg tggtcggtga
     7021 cctggcccgg cgcggcgggg actcgcggtt gccgtgagcc cgcccgcgcg gcagacctca
     7081 gtaccgggcg ctggccgctt cgtcgacgat ccagactcgc tcgagcggcg cgggttcgtc
     7141 cagcgctcgg cgcatccgac cgatcgacgg gttcaccgat gccgagctgc ggagctacgt
     7201 cgagctcctc cagcggatcc aggacagcct cgcgtcggcg gccgagagcg aggactgacc
     7261 gtcctccctt cggtggtggc ggtcaacggc gagcgcgagc cgcgttctcg gtcaaccgga
     7321 ccgcgacgac cttcgtcacg agcgcaaccg ggatcggccg atcgaccggg aactggatcg
     7381 tccccttgcc ggcgaggacg gcgcaagctc ctcgccgagt gcctcgacca ccgctccgct
     7441 cgcaggaaac aggctgcagt gacgcttgta ggccgcgtat gacaccagga actgcccgcc
     7501 gtggctccgg aaggcgggca tcgagtaggc gatcgtctcg gtcgcatcgg gtgcggcaac
     7561 cttgatcgtc tgccgaagga ggtccacggc cgcccgccga tcgtcgggca gggaggccag
     7621 gtagtcgtcg atactcgtcg gtgaggccat gctcgtctcc ttcgtcaacg tgcgtccaca
     7681 tcgatggacg gcgccgggtc aacaggggcc tgctcctgct ggagcacgta gacgttcccg
     7741 tccggagccc ggaagaagag ccagccataa ccggggtctt gcgaactgcc gaacaggtcg
     7801 gccgcccaga ccacgtcggc gacgatctcg atcccggccg ccaacagctc ggcccgagcg
     7861 gcgagcagat cgtcgaccgc gaacgagacg gtcgggccat gggccgcagc cggaaggaga
     7921 cgctcgtcgt actggcccgg tcggtagacc tcgacgaagt cgcggtcgcc cgactcgagc
     7981 gaaaagacgc cccagccagc gtcaagatga gccgacggca acccgagcac gtcccggacg
     8041 aacccggtca tggcatcgaa gttctcggtc cggacgccga cgaaacagac gcgactgatg
     8101 cgcacgatgg aacctccggg agccgccacg atcgatcctg ggcgcgagcg tcgtgggagg
     8161 agacgaggcg ccggtgcgcc aggtgtcggc caatgcccat ccctgttcca tcagccagac
     8221 ccgcacagga tgacccagcc cgtgatcggt tcgatgcaga gcaacatcgc cgacttgtag
     8281 ccgggccggt catctgtggc tccttcacgt cgccgtcgat gctcacgccc tggaacggag
     8341 ggatgagcgg agacaagcct ccagtaccaa cctctttgtc ccagcttctt tgctgctgga
     8401 gatcgaggtt agcggggtgg agcgctggct gagccgctcg actgcctgac ggtcgcagtg
     8461 accctcaagc ccagtgcgct gcacgatgcg agcccgtccc ccgcaagctc ctcgaaggtc
     8521 gccgctcgcg cttcgcttgc gcggacatac cggagcgcgg gcccagtcca agaccgatga
     8581 agggcaagtc ggctgcggcg gttgggtctg gcgaccgata atccggtc
//
LOCUS       DATAIW010000016        12575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_421, whole genome shotgun sequence.
ACCESSION   DATAIW010000016 DATAIW010000000
VERSION     DATAIW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3111)
                     /locus_tag="VHM48_00570"
     CDS             complement(<1..3111)
                     /locus_tag="VHM48_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753921.1"
                     /translation="MASRGLWHFMGARTRRSLALSWSALFVLSLLLQYFTFALAPAAL
                     AVHDEDLFELDGNALNDAAVTGDDWNSHPGATGNRFTFIDDLYNSSLDIGFAGGGSKD
                     DLNTSSWAWLQDTVTPDKDDIEHAYAASYVKDGHTFVYFGLDKLAVNGDSNVGFWFFK
                     NGIGLTGGPTSGGFAPQHTVGDLLVLSQFTNGGVVSTIDLYEWVGSGGSNGTLDKIAA
                     GNACTGSPAVDKACAIANTAPTAAPWAYHPKSGPDNIFPTASFFEGGIDLDQLFAPGK
                     APCFSGFLADTRTSQDVNAVLKDVAVGQFNTCVPPTITTDASVSTFHFGDAAVTDTAT
                     LSGSDGPASGKVKFFVCNPADITAAGCPKGSGTQVGAAAGVTVTTSTNGGSATSAGFT
                     PTAAGKYCFRAEYIPDAASQYLAASHTNATTECFTVVKNPTAITTAAEQTVNLGQAIA
                     DSATLSGATGDAGGSIVFRAYGPADVTCANTPAFVSAPFAVSGNGTYGAASFIPTTAG
                     TYRWIASYGGDAKNLASIGSCNDAGENDVVNKLIPTIATQASADVIVGGQIHDTATVS
                     GGQNPTGTVTFNLYGPNDTSCGSAAIFTSGPIALSNGSATSGNFTTTQVGTYRWRATY
                     NGDVNNNPVTGACNAANENVVVSPASPAITTSLSGDGRTGASITVPLGTPVHDSSTLS
                     GATATAGGTVHYQVFSDPQCLVLLSDAGTKTVVNHIVPNSNDITFIHAGTYYWQADYS
                     GDPNNNAASSACNLETVTVDKNIPTIVTNASGTVQVGGQIHDSATLSGGFSPTGSITF
                     KLYGPNDATCTGTVAFTSTVTVNNGNDTYASGNFTTTAAGTYRWVASYSGDGDNAAVS
                     GGCNDNNESVVVTKRNPTVVTTASASVQVGNAISDSALLAGGYQPTGSITFNLYGPAD
                     NNCTGAVKFTATITVSGNGTYGSGNYTATAAGTYHWIANYSGDTNNNATANGCNEANE
                     NVIVTPRNPTVVTTASASVEVGGVISDSALLAGGFNSTGSITFNLYGPNDLNCTGAVK
                     FTATVTV"
     gene            3508..5208
                     /gene="argS"
                     /locus_tag="VHM48_00575"
     CDS             3508..5208
                     /gene="argS"
                     /locus_tag="VHM48_00575"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2753922.1"
                     /translation="MTSPSQPTTVAPIRTQVREAVARAWQRAIEAEALPIVEDGLPVV
                     EIERPASPDHGDFAASLALKLARPYRRAPLDIARALADAIAADAADAASASPIASVDV
                     APPGFLNLRLTDTALEGTVATVLVDPAGWGRVPAVRPRTVNVEFVSANPTGPLTIGNA
                     RGAFVGDLLCRVLEAGGQAVTREYYFNDSGAQVSNLGASVVAVRRGEPIPDDGYHGAY
                     VEDLAHDLPDEVLAAAEQPGAEAADVVGRWAAARVRAGIEASLERLGVHFDVWKSEGS
                     LHTEGWVERAVQRLRGGGHVYEQDGAIWFRSTTFGDDKDRVIYRSDGRPTYFAADIGY
                     VTEKFSRGFDHLIYIWGADHHGTVARVRNAATAMGYDAAAVQVLLYSWVRFIRDGQEI
                     SMSKRAGEFITLDELLAEVGVDAARWFFASRAATTGIDFDIELAKKQSADNPVFYVQY
                     AHARIASILRKAVGVGLAPATNVVGVLSGGPEAALARAIARFPEVVEDAARSEETHGV
                     TAYATELATQFHAFYRDARVVDADEPVRSAGRLALVSAARVTLANALALLGISAPESM
                     "
     gene            complement(5385..6695)
                     /locus_tag="VHM48_00580"
     CDS             complement(5385..6695)
                     /locus_tag="VHM48_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753923.1"
                     /translation="MHRQAAFAKAVHRRPRRLGSLLACLALIAAACGSSGPSGSTAAT
                     TPATTPGVSASPTPFVDPATVYASIEDQVVAIRGLKPTTKVDPKILDDAGIKKLTADS
                     FARDNPPATIAANERILKALGLLPADASLTDLYVNLLGSQVAGLYNPDDKTLYVVSRS
                     GGLGPAQKTTFAHEFTHALQDQNFDIGSLKLDEIGQGDRSFARLSLVEGDATLLMSLW
                     QIQNLTQADLGQLLAEAGSDPSTKVLLDMPPILREALLFPYTGGLSFVQGLQGSGGWQ
                     AVNDAFSKPPASTEQILHPEKYASGEAPIAVTLPKDLVTSLGGGWKVALEDSFGEFQL
                     AVWLRGSKAIDAATANDAAAGWGGDRIALVEGPDGAWGVILRTAWDSAADAAAFEAAA
                     ASLVDGLANPASLLPGSGGAERWIVIGSDDATLNRVAGALGLAG"
     gene            7036..7707
                     /locus_tag="VHM48_00585"
     CDS             7036..7707
                     /locus_tag="VHM48_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2753924.1"
                     /translation="MELTWYGRTCIRLRGKDAVVVADPYQSIVGPTGRGITGEIVTYS
                     HPDDRPLPKAKGHRSRDGLTLLPSSLDDAFVLDGPGEYEVRDVLLTGVRTYRDDAKGS
                     ERGKQIAFVVELDGMHTIHLGEIGHLLTEEKLADIGPVDIACVPLGGALSPQRVAELV
                     AQLDPKIVVAMPLDEDAPDTREALAKFFHEMGGEPAVQPKLTVTISGLPAETTTVLLE
                     SRGKI"
     gene            complement(7908..9272)
                     /gene="hutI"
                     /locus_tag="VHM48_00590"
     CDS             complement(7908..9272)
                     /gene="hutI"
                     /locus_tag="VHM48_00590"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX2753925.1"
                     /translation="MTFRVIPGGRAERDQPGLLVVGAAEVVTMAGGPRRGEEQGAVDR
                     REAASTGGLGGPNAPAVAIWQGRILGVGGRADLERVLEAEGYPPERFDRLDAAGGTVT
                     PGLIDPHTHLLFAGSREGELELRQQGAGYLEILAAGGGILSTVAATRLASADELAVHG
                     RRWLDQMLGHGVTTVEAKSGYGLDLPTELRLLEVAHQLGTEGPIEVVPTWLGAHAVPP
                     EMRNRPDGTEAYVRSLIDIQLPGVAAQGRAVAADVFCERGVFTAEQSRRILTAAAGYG
                     LRTRLHADELAPSGGAELAAEIGATTADHLATPSEAGIAAMARASAEGHPVVATLLPA
                     TTWFLMKDRHAPARAFIEQGVPVAIGTDFNPGTSPTPSLPLAMTAACLNLRMTPDEVL
                     AAVTINAALALGLADEVGSLEGGKQADLVIWAVPTSRQIPYWPAADLVRTVVKRGRIV
                     FDRS"
     gene            complement(9269..10273)
                     /gene="ftcD"
                     /locus_tag="VHM48_00595"
     CDS             complement(9269..10273)
                     /gene="ftcD"
                     /locus_tag="VHM48_00595"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX2753926.1"
                     /translation="MPAVPTSLDDRLVESVPNVSEGRRLDVVERLATAITSVPGVHLL
                     DRTSDASHNRSVFTLAGPAGAVCDALEQLVAAAVREIDMDEHSGEHPRIGAVDVIPFV
                     PLADTTMDEAVELARAFGARIADRFDLPVYLYARAARRGDRIKLADVRRGQYEGLKVE
                     IGQNGREPDFGPARMHPSAGAVAVGARPFLIAYNINLESEDVELAKRIARRVRESGGG
                     LPKVQANGFFIDELRRAQVSMNILDFRVTPLWRVWETVRAEAAEDQIELFESELIGLA
                     PLAAFIDVADHVETPAGAPIEERLAAAAAFLQLRDFSPLQALELRLEAARSTTDLRRP
                     "
     gene            10417..11823
                     /locus_tag="VHM48_00600"
     CDS             10417..11823
                     /locus_tag="VHM48_00600"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2753927.1"
                     /translation="MAASTAPATPRGGLFRHPNYLKLWTAATVSLFGTQVSQIAIPFI
                     AAVVLKASAGEVGLLTTIEFLPFILFALPAGVWVDRFPKRRILVIGDLGRALMLISIP
                     IASALGSLTIWQLYLVGFVNGAMTVFFDVADQSFLPAILDRGELVEGNSKLQISQSSA
                     QILGQPVGGGLVALLSAPVAVLIDAASYFGSAALILWIRLTSGGARSTGRRSDIARNA
                     AGEAIGDAGTATSPTAGASLAAASGDTPVASDEDGGMRGQIAAGLGYIGHHRFLASIA
                     ASTATSNLFSNIAFAILPVYLYTTLGLSPASVGAIGGIGGAGVLIGALLASRIAKAFG
                     VGRVIVGSLLLGGLAGLLVPAAPPELAFWFVATAFFIGSIAGVVYNVNQVSLRQAITP
                     EHFLGRMNATMRFLVWGTIPIGSLIGAGLSEVIGVHMTIWVGATLGVFSFLPVLLSPV
                     RSLREIPTDDPDEAATTS"
     gene            complement(11929..>12575)
                     /locus_tag="VHM48_00605"
     CDS             complement(11929..>12575)
                     /locus_tag="VHM48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX2753928.1"
                     /translation="AHGDAEAFDDRVRAAGVDRRISVDHHRRVVVSATGADPENGSLT
                     YAWDLDNNGSFETPGQSVTFNAPPDSAPSSPTIRVRVTDPLGLTATDTATVDIIWPFN
                     GFLAPLSNPPTENTANAGSGLPIKFMLGGNQGLAVIAAGYPTSVAYTCGIPVGSRPTD
                     ASTPTSGGGFTYDAATGIYNYNWKSQKSWSNTCRRFVLKLTDGTFHYVDVHFVK"
BASE COUNT         1805 a         4203 c         4519 g         2048 t
ORIGIN      
        1 gaccgtgacg gtggcggtga acttcaccgc gccggtgcag ttgaggtcgt tcgggccata
       61 gaggttgaag gtgatcgagc cggtcgagtt gaacccgccc gccaggagcg cgctgtcgga
      121 gatgacgccg ccgacctcga ccgaggccga ggccgtggtc acgaccgtcg ggttgcgcgg
      181 cgtgacgatc acgttctcgt tggcttcgtt gcaaccgttg gcggtggcgt tgttgttggt
      241 gtcaccgctg tagttggcga tccagtggta ggtcccggcc gcggtcgccg tgtagttgcc
      301 ggagccgtag gtcccgttgc cgctgacggt gatggtggcg gtgaacttca ccgcgcccgt
      361 gcagttgttg tcagctgggc cgtagaggtt gaaggtgatc gagccggtcg gctgatagcc
      421 gcccgccagg agcgcgctgt cggagatcgc gttgccgacc tggaccgagg ccgaggccgt
      481 ggtcacgacc gtcgggttgc gcttggtgac gacgacgctc tcgttgttgt cgttgcagcc
      541 accggacacg gcggcgttgt cgccgtcgcc gctgtagctc gcgacccagc ggtatgtccc
      601 cgcggcggtc gtcgtgaagt tgcccgaggc ataggtgtcg ttgccgttgt tgaccgtgac
      661 ggtgctggtg aacgccaccg tgccggtgca ggtcgcgtca ttcgggccgt agagcttgaa
      721 ggtgatcgag ccggtcgggc tgaagccacc ggacagggtc gcggagtcgt ggatctggcc
      781 gccgacctgg accgtgccgg aggcgttggt cacgatcgtc gggatgttct tgtcgacggt
      841 gaccgtctcg aggttgcagg cgctcgacgc cgcgttgttg ttcgggtcgc cgctgtagtc
      901 ggcctgccag tagtaggtcc cggcgtggat gaaggtgatg tcgttggagt tcgggacgat
      961 gtggttcacg accgtctttg tgccggcgtc cgaaaggagg accaggcact gaggatcgct
     1021 gaagacctgg tagtggacgg tgccgccggc cgtggccgtg gcgccgctca gggtcgagct
     1081 gtcatggacc ggcgtcccga gcggaacggt gatcgaggcg ccggtcctgc cgtcgccgga
     1141 cagcgacgtc gtgatcgccg ggctcgccgg gctgacgacg acgttctcgt tcgccgcgtt
     1201 gcaggcgccg gtgacggggt tgttgttgac gtcgccgttg tacgtcgctc gccagcggta
     1261 cgtcccgacc tgggtggtcg tgaagttgcc ggacgtcgcc gagccgttgc tcagcgcgat
     1321 cgggccgctg gtgaagatcg ccgcgctgcc gcagctggtg tcgttggggc cgtacaggtt
     1381 gaacgtgacg gtgccggtcg ggttctggcc gcccgagacc gtggcggtgt cgtggatctg
     1441 gccgccgacg atgacatcgg ccgaggcctg ggtcgcgatc gtcgggatga gcttgttgac
     1501 gacgtcgttc tcgccggcgt cattgcacga gccgatcgag gcgaggttct tggcgtcgcc
     1561 gccgtacgag gcgatccaac ggtaggtgcc ggccgtcgtc gggatgaacg aggcggcgcc
     1621 gtaggtcccg ttgccgctga ccgcgaacgg agcggaaacg aacgccgggg tgttggcgca
     1681 ggtgacgtcc gccgggccgt acgcccggaa gacgatcgag ccgccggcgt ccccggtcgc
     1741 acccgacagc gtcgcgctgt ccgcgatcgc ctggcccaga ttgaccgtct gctccgccgc
     1801 cgtcgtgatc gcggtcgggt tcttgacgac ggtgaagcac tcggtggtcg cgttggtgtg
     1861 gctcgcggca aggtattgcg atgccgcatc cgggatgtac tcggcccgga agcagtactt
     1921 gcccgcggcc gtcggcgtga agccggccga tgtcgcggat ccgccgttcg tggaggtggt
     1981 cacggtgacg ccggcggcgg cgccgacctg ggtgccgctg cccttcgggc agccggcggc
     2041 ggtgatgtcg gccggattgc agacgaagaa cttgaccttg ccggaggcgg ggccgtcgct
     2101 gccggacaga gtcgcggtgt cggtcacggc cgcatcaccg aagtggaagg tcgagaccga
     2161 ggcgtccgtc gtgatcgtgg gcggaacgca ggtgttgaac tggccgacgg caacatcctt
     2221 caggacagcg ttgacatcct gcgaggtgcg cgtgtcagcg aggaagccgg agaagcacgg
     2281 cgccttgccc ggggcgaaga gctggtcgag atcgatgccg ccctcgaaga acgaggcggt
     2341 cgggaagatg ttgtccgggc ccgacttggg gtggtacgcc cacggggcgg cggtcggggc
     2401 cgtgttggcg atcgcgcagg ccttgtcgac cgcgggggaa ccggtgcacg cgttgcccgc
     2461 ggcaatcttg tccaaggtcc cattggagcc gcccgagccg acccactcat acaggtcgat
     2521 cgtgctgacg acgccgccgt tggtgaactg gctcaggacg agcaggtcgc cgaccgtgtg
     2581 ctgcggggcg aagccgccgc tggtcggacc gcccgtcaga ccgatgccgt tcttgaagaa
     2641 ccagaagccg acgttcgagt cgccgttgac ggccagcttg tcgaggccga agtagacgaa
     2701 cgtgtgaccg tccttgacgt acgaggccgc gtacgcgtgc tcgatgtcgt ccttgtccgg
     2761 tgtgacggtg tcctgcagcc atgcccagga cgaggtgttc aggtcgtcct tggagccgcc
     2821 gccggcaaag ccgatgtcaa gggagctgtt gtagaggtcg tcgatgaagg tgaaccggtt
     2881 accggtcgca cccggatgtg agttccagtc gtcgcccgta acggcggcgt cgttgagggc
     2941 gttgccgtcg agctcgaaca ggtcctcgtc gtggacggcc agggccgccg gcgcgagggc
     3001 gaaggtgaag tactgcaaca gcaaagacaa cacgaagagc gccgaccacg acagagccag
     3061 gctccgccgc gtccgcgctc ccatgaaatg ccacagtccc cgagatgcca tgttcctgtt
     3121 cctcccgatc cccgagaacg aaaccacgat agataagacc tcgcgaaaga gatggacccc
     3181 cttgaggttc ccgaatggga cctcgagtcc gttcaagccg gcttggggcc ggtacttccg
     3241 gttcccctga agaaccctcc cggacgggag gacacggcca ctctacggct gaacggatag
     3301 gaaaaggtac tggtacggat tgcgcatgac cagtcccaaa tgcactgttg tccaccgttt
     3361 gacacaaccg cggaaagcgc attccggtgc gagcgtgcac cgaggtccca ggaccgatgg
     3421 cggacgaggc tggcgcgcta ccatgggcga cccatcgcgt cgcatggcgg acgctcacgc
     3481 ccacccccca gcgaggcctc gccgatcgtg accagcccga gccagccgac gacagtggcg
     3541 cccatccgga cgcaggttcg cgaggccgtg gcccgggcct ggcagcgagc gatcgaggcg
     3601 gaagccctgc cgatcgtcga ggatggcctg ccagtcgtcg agatcgagcg gccggcgagc
     3661 ccggaccacg gcgatttcgc cgccagcctc gcgctgaagc tggcgcgacc gtatcgccgc
     3721 gcaccgctcg acatcgcccg cgccctcgcc gacgcgatcg cggcggacgc ggccgatgct
     3781 gccagcgcct cgccgatcgc ctcggtcgat gtcgcgccgc cgggcttcct gaaccttcgc
     3841 ctgaccgaca cggcgctgga gggaacggtg gcgacggtcc tggtcgatcc cgccggctgg
     3901 gggcgtgtcc ccgcggtccg gccacgaacc gtcaacgtcg agttcgtgtc ggccaacccg
     3961 accgggccgc tgacgatcgg caatgcgcgc ggcgctttcg tcggcgacct cctctgccgg
     4021 gtcctcgagg cgggcggcca ggcggtcacg cgcgagtatt acttcaacga ctcgggagcc
     4081 caggtcagca acctgggggc atcggtcgtc gcggtccggc gtggggagcc gatccccgac
     4141 gacggctacc acggcgccta tgtcgaggat ctcgcgcacg acctgccgga cgaggtcctc
     4201 gccgcagccg agcagcctgg agccgaagca gccgacgtcg tcgggcggtg ggccgcggcg
     4261 cgggtccggg ccggcatcga ggcgagcctc gagcggctcg gcgtccattt cgacgtctgg
     4321 aagagcgaag gctcgctcca caccgagggt tgggtcgagc gcgccgtcca gcgcctccgt
     4381 ggcggcggtc atgtctacga gcaggacggc gcgatctggt tccgctcgac gacgttcggc
     4441 gacgacaagg atcgcgtgat ctatcggtcc gacggccgcc cgacctactt cgccgccgat
     4501 atcggctacg tcaccgaaaa gttcagccgc ggcttcgacc acttgatcta catctggggg
     4561 gcggaccatc acgggacggt ggcgcgggtc cggaacgcgg cgacggcgat gggctacgac
     4621 gcggccgccg tccaggtcct cctctactcc tgggtccgct tcatccgcga cggccaggag
     4681 atctcgatga gcaagcgggc cggcgaattc atcaccctcg acgagctcct cgccgaggtc
     4741 ggcgtcgatg ccgcccgctg gttcttcgcc tcacgggcag cgacgaccgg gatcgacttc
     4801 gacatcgagc tcgcgaagaa acagtcggcc gacaacccgg tcttctatgt ccagtacgcc
     4861 cacgcccgga tcgcttcgat cctgcgcaag gcggtcggcg tgggtctggc cccggcgacc
     4921 aatgtcgtcg gggtgctgag cggtgggccg gaggcggcgc tcgcccgggc aatcgctcgg
     4981 ttcccggagg tcgtcgagga cgcagcacga tccgaggaga cccacggcgt cacggcctac
     5041 gcgaccgagc tcgcgaccca gttccacgcc ttctaccgcg acgcgcgggt ggtcgacgcc
     5101 gacgagccgg tgcgctccgc gggccgcctc gccctcgtgt cggctgcccg ggtcactctc
     5161 gccaacgccc tcgcgctgct cgggatctcg gctccggagt cgatgtaggc ctgggtcggc
     5221 cgtgcctcgg gccggtcgat cggccggtcg gccaagcggt tcggtcgccc gacgcgggtt
     5281 cggtcgcccg acgcgggttc ggtcgcccca cgcccgaacg gggatcggca ccccgggccg
     5341 ccggcacccg gccggcaggc acgccggcac atcgcggccg agcctcagcc ggccagaccg
     5401 agagcgccgg cgacgcggtt gagcgtggcg tcgtcggagc cgatgacgat ccagcgctcc
     5461 gctccaccgc ttcccggtag cagggaggcc gggttcgcga gaccatcaac gagagacgcg
     5521 gcggcggctt cgaaggccgc cgcgtcggcg gcgctgtccc aggccgtccg gaggatgacg
     5581 ccccaggcgc cgtccggtcc ctcgacgagg gcgatccggt cgccacccca gccggccgcc
     5641 gcatcgttcg ccgtggcggc atcgatcgcc ttgctgccgc ggagccagac ggcgagctgg
     5701 aattcgccga acgagtcctc gagagcgacc ttccagccgc cgccgaggct cgtcacgagg
     5761 tccttcggga gggtcaccgc aatcggcgcc tcgccgctgg cgtacttctc ggggtggagg
     5821 atctgttcgg tcgaggcggg cggcttgctg aacgcatcat tgacggcctg ccagccgccg
     5881 gacccctgga ggccctggac aaagctgagg ccgccggtgt acgggaagag cagtgcctct
     5941 cggaggatcg gcggcatgtc caggaggacc ttggtcgacg ggtcgctgcc cgcctcggcg
     6001 aggagctgcc cgagatcggc ctgggtcagg ttctggattt gccagaggct catcagcagg
     6061 gtcgcatcgc cttcgaccag cgacagtctc gcgaacgacc gatcgccctg gccgatctcg
     6121 tcgagcttga gcgagccgat gtcgaagttc tggtcctgga gcgcgtgggt gaactcgtgg
     6181 gcgaacgtcg tcttctgggc cggcccgagc ccgcccgacc gagacacgac atacagcgtc
     6241 ttgtcgtcgg ggttgtagag gccggcgacc tggctgccga gcaggttgac gtagaggtcc
     6301 gtgagtgacg cgtcggcggg caggaggccg agcgccttga ggatccgctc gttcgcggcg
     6361 atggtcgccg gcgggttgtc cctcgcgaac gaatcggccg tcagcttctt gatcccggcg
     6421 tcatcgagga tcttcgggtc gaccttggtc gtgggcttca agccgcggat cgcgaccacc
     6481 tggtcctcga tcgacgcgta gacggtggcc ggatcgacga agggcgttgg cgaggcactg
     6541 acgcccggcg tcgtggccgg cgtcgtggcg gccgtcgatc ccgacggtcc gctcgagccg
     6601 caggcggccg cgatcagcgc caggcaggcg aggagcgagc cgaggcggcg tggacggcgg
     6661 tggacggcct tcgcgaaggc tgcttggcgg tgcatcggcc ggattgttgc acgggtcgtg
     6721 gcggtggacg atggcccggc agtagggggt tccatgtcgc tttcttaatg gcttccccgg
     6781 ttgtcgttga acgcgtcagg tctagcgttc gtttcggacc gaaccctcag gggttccatc
     6841 ctccctccct ccgaccgggg gcgccgattg ggcaggcgtc cccggtcatt ctttgccacc
     6901 ctcgcccccg cgcgacgaac ggagtcacgc gacccttggc gggctgtcgc gttttcgcgg
     6961 gcgatcgggc atccccggaa tggccgattc cgggtgatgc ttcgcggtgc aggtgcgtgt
     7021 gcaagaatca attccatgga actgacctgg tacggacgga catgcattcg tctccgcggc
     7081 aaggacgccg tcgtggtggc cgacccctat caatcgatcg tcgggccgac cgggcgcggc
     7141 atcaccggcg agatcgtcac ctactcgcac ccggacgacc gtccgctgcc aaaggccaag
     7201 ggccatcgtt cgcgcgacgg cttgaccctc ctgccctcat ctcttgacga cgccttcgtc
     7261 ctggacggcc ccggcgagta cgaggtccgt gacgtcctgc tcaccggcgt gcggacgtac
     7321 cgcgacgatg cgaagggctc ggaacgcggc aagcagatcg ccttcgtggt cgagctcgac
     7381 gggatgcaca cgatccacct cggggagatc ggccatctgc tgaccgagga gaagctcgcc
     7441 gatatcggcc cggtcgacat cgcgtgtgtc ccgctcggcg gcgcgctgtc gccgcagcgg
     7501 gtggccgagc tcgtcgcgca gctcgacccg aagatcgtcg ttgcgatgcc gctcgacgag
     7561 gacgcccccg atactcgcga ggcgctcgcg aagttcttcc acgagatggg cggcgagccc
     7621 gccgtccagc cgaagctgac ggtgacgatc tcggggctcc cggccgagac gacgacggtg
     7681 ctgctcgagt cgcgcggcaa gatctgagct ccatcgacct tccgcccgat cgccgcgtgc
     7741 gctcggcggt ccacgttcgg tcggcccgcc cccgtttcgg tagccgaata ttgggcgctg
     7801 ccgagagcgg gccaacgcga tctgcccagg gctcgatatt cggctattcg cagtgccccg
     7861 gcgctgtcct tggctggcag cccgtacggc gcgaagcgcc gctgccgcta cgaccggtcg
     7921 aagacgatcc gcccgcgctt gacgaccgtg cggacgagat cggccgccgg ccagtacggg
     7981 atctggcgtg aggtggggac cgcccagatc acgagatcgg cctgcttgcc gccctcgagc
     8041 gagccgacct cgtcggccag gccgagggcg agcgccgcgt tgatcgtcac cgccgccagc
     8101 acctcgtcgg gcgtcatccg caggttcagg caggcggccg tcatcgccag cggcaggctc
     8161 ggcgtcggcg atgttccggg gttgaagtcg gtcccgatcg cgaccgggac gccctgctcg
     8221 atgaacgcgc gggcgggcgc atggcggtcc ttcatgagga accaggtggt ggccgggagc
     8281 agcgtcgcga cgaccggatg gccctcggcc gacgcccggg ccatggccgc gatgcctgcc
     8341 tcggacgggg tcgcgaggtg gtcggcagtg gtcgccccga tctcggccgc gagctccgcg
     8401 ccgccgctcg gtgccagctc gtcggcatgc aaccgcgtcc gcagcccgta gcccgccgcc
     8461 gcggtcagga tccgtcgcga ctgctcggcc gtgaagactc cccgctcgca gaacacgtcg
     8521 gccgcaaccg cccgtccctg ggccgcgacg cctggcaact gaatgtcgat cagggagcga
     8581 acataggcct cggttccgtc cggccggttg cgcatctccg ggggaaccgc gtgggccccg
     8641 agccaggtgg ggacgacctc gatcggcccc tccgttccca gctgatgggc gacctcgagc
     8701 aggcggagct cggtcggcag gtcgagacca tacccggact tggcctcgac ggtcgtgacc
     8761 ccgtggccga gcatctggtc gagccagcgg cggccgtgaa cggcgagctc gtccgccgag
     8821 gccaggcgcg tcgccgcgac ggtcgagagg atcccgccgc cggcggccag gatctcgagg
     8881 tagcccgcgc cctgctggcg gagctcgagc tcaccttcgc gactgccggc gaagaggagg
     8941 tgggtgtgcg ggtcgatcag gcccggggtg accgttccgc cggccgcgtc gagtcgatcg
     9001 aaccgctcgg gcgggtagcc ctccgcttcg aggacgcgct cgaggtcagc gcgtccgcca
     9061 accccgagga tccggccctg ccagatcgcg acggccggag cgttcggtcc cccgagtcca
     9121 ccggtcgagg cggcctcacg gcgatccacg gcgccctgct cctcgccgcg gcgcggcccg
     9181 ccggccatcg tgaccacctc ggccgccccg acgacgagca gccccggctg gtcccgctca
     9241 gcacgtccgc ccgggatgac gcggaaggtc acgggcggcg aaggtccgtc gtggaccggg
     9301 ccgcctcgag gcggagctcc agggcctgga gcgggctgaa gtcgcgcaat tgcaggaagg
     9361 ccgcagccgc cgccaggcgc tcctcgatcg gcgctccggc cggcgtctcg acgtggtcgg
     9421 cgacgtcgat gaaggcggcg agcggagcaa ggccgatcag ctccgactcg aacagctcga
     9481 tctggtcctc ggccgcttcg gcccggaccg tctcccagac ccgccacagc ggcgtcaccc
     9541 ggaagtcgag gatgttcatc gagacctggg ccctccggag ctcgtcgatg aagaagccgt
     9601 tggcctggac cttgggcaac ccgccgcccg attcccggac gcggcgggcg atccgcttgg
     9661 caagctcgac gtcctcactc tcgaggttga tgttgtaagc gatcaggaac ggccgcgcgc
     9721 cgaccgccac ggcacccgcg gacgggtgca tccgggccgg cccgaagtcc ggctcgcggc
     9781 cgttctggcc gatctcgacc ttcaggcctt cgtattgccc gcgccggaca tcggccagct
     9841 tgatccgatc gccgcgccgc gcggcccggg catagaggta gaccggcagg tcgaatcggt
     9901 cggcgatccg ggcaccgaac gcccgggcca gctcgaccgc ctcgtccatc gtcgtatcgg
     9961 cgaggggcac gaacgggatg acgtcgaccg ccccgatccg cggatgctcg cccgagtgct
    10021 cgtccatgtc gatctcgcgg acggccgccg cgaccagttg ctcgagggcg tcgcacacgg
    10081 cgcctgcggg accggccagg gtgaacaccg agcggttgtg gctggcgtcg ctggtccggt
    10141 cgagcaggtg gacgccgggg accgacgtga tcgccgtcgc gaggcgctcg acgacgtcga
    10201 gccgtcgtcc ctcggagacg ttcgggacgg attcgaccag acggtcgtcc agggaggtgg
    10261 gcacagcggg catcgcgtcc atcgtgccac ggttccggtc gggcaacgca tctgcacatg
    10321 ttgactaact cgtgttagat cgtaaactaa cgccgcgttg gtcgagatgc cggacttgcc
    10381 ggtgcgccgg ccgtgtcccg cagcgcgagg tgcccgttgg ccgcgtcgac cgcccccgcc
    10441 actccccgcg gtggcctgtt ccgccatccg aactacctga agctctggac cgcggcaacg
    10501 gtctcgctct tcgggacgca ggtcagccag atcgcgatcc cgttcatcgc cgcggtcgtc
    10561 ctgaaagcct cggccggcga ggtcgggctc ctgacgacga tcgagttcct gccgttcatc
    10621 ctgttcgccc tgccggccgg ggtctgggtc gatcgcttcc cgaagcggcg gatcctcgtg
    10681 atcggggacc tcggacgggc gctgatgctg atttcgatcc cgatcgcctc cgccctcgga
    10741 tcgttgacga tctggcagct ctatctcgtc gggttcgtga acggggcaat gaccgtcttc
    10801 ttcgacgtcg ccgaccagtc gttcctgccc gcgatcctcg atcggggcga gctcgtcgag
    10861 ggcaactcga agctccagat cagccagtcg tccgcgcaga tcctcggcca gccggtcggt
    10921 ggcggcctcg tggccctgct gagcgcgccg gtcgcggtcc tgatcgacgc ggccagctat
    10981 ttcggctcgg ccgcgctcat cctgtggatc cggctgacgt cgggcggggc ccgctccacg
    11041 ggacgccgct ccgacatcgc aaggaatgct gccggagagg cgatcgggga cgccgggacg
    11101 gccacgtcgc cgacggcggg cgcgtccctc gcggcggcga gcggcgacac gccggtcgca
    11161 agcgacgagg atggcggcat gcgcggccag atcgcggctg gcctcgggta catcggccac
    11221 catcgattcc tcgcgagcat cgccgcctcg accgcgacgt ccaacctgtt ctcgaacatc
    11281 gcgttcgcga tcctgcctgt ctacctgtac acgacgctcg gcctgtcacc cgccagcgtc
    11341 ggcgcgatcg gcgggatcgg cggggccggc gtgctcatcg gtgccttgct cgcgagtcgg
    11401 atcgcgaagg ccttcggggt cggtcgggtc atcgtcgggt cgctgctcct cggcgggctc
    11461 gccggcctgc tcgtccccgc cgcgccgccg gagctcgcct tctggttcgt ggcgaccgca
    11521 ttcttcatcg gaagcatcgc gggggtcgtc tacaacgtga accaggtcag cctccgccag
    11581 gcgatcacgc cggagcactt cctcgggcga atgaacgcca cgatgcggtt cctggtctgg
    11641 ggcacgatcc cgatcgggtc actgatcggg gcggggctct cggaagtcat cggcgtccac
    11701 atgacgatct gggtcggcgc caccctcggc gtcttctcgt tcctgccggt gctcctgtcg
    11761 ccggtccgct ccctgcggga gatcccgacc gacgatccgg acgaggcggc gacgacctcc
    11821 tgactgtcgc ggggaccggt tcccgggtcg atcgcgaccg gcagccgggc aacgcgaagg
    11881 gccggccacg acggggccgg cccttgtcga ttcaggacgc ggaaccggtc atttcacgaa
    11941 atggacgtcc acgtagtgga aggtcccatc ggtcagcttc aggacgaagc gccgacacgt
    12001 gttcgaccag ctcttctgcg atttccagtt gtagttgtag atgcccgtgg ctgcatcgta
    12061 ggtgaagcca ccgccggagg tgggcgtgct cgcatcggtc gggcgtgacc cgaccgggat
    12121 gccgcaggtg tatgcgaccg aggtcggata tccggcagcg atgacggcga ggccctgatt
    12181 gccgccgagc atgaacttga tcggcaggcc ggatccggcg ttggctgtgt tctcggttgg
    12241 cgggttcgag agcggggcaa ggaacccatt gaacggccag atgatgtcca ccgtcgccgt
    12301 gtcggtcgcg gtcaggccga gcgggtcggt gacacggaca cggatcgtcg gcgacgatgg
    12361 ggcagagtcg ggcggggcgt tgaatgtcac cgactggccc ggggtctcga acgagccgtt
    12421 gttgtcgagg tcccaggcgt aggtcagcga accgttctcc ggatcggcgc cggtggcgct
    12481 gacgacgacg cgacggtggt ggtcaacgct tatccgacgg tcgacgccgg cggcccgtac
    12541 acggtcgtcg aaggcttcgg cgtcaccgtg agcgc
//
LOCUS       DATAIW010000017         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_89875, whole genome shotgun sequence.
ACCESSION   DATAIW010000017 DATAIW010000000
VERSION     DATAIW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89875"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VHM48_00610"
     CDS             <1..256
                     /locus_tag="VHM48_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX2753929.1"
                     /translation="GYLGYDGNLDTAITIRSAVLKDGLAHVHTGAGIVAGSIPEREFE
                     ETEHKAAALRRAIEMAAGVAEAPPSDDEAAIEADRARARP"
     gene            253..942
                     /locus_tag="VHM48_00615"
     CDS             253..942
                     /locus_tag="VHM48_00615"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX2753930.1"
                     /translation="MILVIDNYDSFTFNLVQALQAAGAEVHVVRNDALDIAAVEAMAD
                     DPDADLRGIVISPGPGGPADAGISVGVVRVAADRGLPLLGVCLGMQSMAAAFGASIVR
                     APTLVHGEASEVTHDGAGLLEGMPPAFMAARYHSLVVDAATLPPELRVTAMSEVDRVI
                     MGIRHVALPLEGVQFHPESVLTPEGPHLLANFLRQAGEGEASRLDDATGSFATAGLAR
                     MSDEPLGEATR"
     gene            939..>1789
                     /gene="trpD"
                     /locus_tag="VHM48_00620"
     CDS             939..>1789
                     /gene="trpD"
                     /locus_tag="VHM48_00620"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX2753931.1"
                     /translation="MSDLVRAALNTIVEGGTLSIDEARVAMGAVMDGEATPAQLAAML
                     MGLRMRGETVDELAGFATAMRERVVHVEAPEGAIDVVGTGGDGSGTFNISTTAALVAA
                     AAGVPVAKHGNRAITSRAGSADVLDALGIRIDHDATSAGAALRELGFAFLFAPNFHPA
                     MKHAGPTRREIGVRTAFNLLGPLTNPAGTRRQLLGVGDAAAAGRMADVVQRLGTDLTF
                     VIHGDGVDELPLDGSGVLYVIAGDTIERHAIDAAALGFKRAATARLSGGSPAENARMT
                     ETVLQGEP"
BASE COUNT          253 a          618 c          675 g          243 t
ORIGIN      
        1 gggctacctg ggctacgacg ggaatctcga cacggcgatc acgatccgga gcgccgtgct
       61 caaggacggc ctggcgcacg tccacacggg cgccgggatc gtggccggat cgattccgga
      121 gcgcgagttc gaggagaccg aacacaaggc ggctgcgctc aggcgcgcca tcgagatggc
      181 ggccggcgtg gcggaggcgc cgccctccga cgacgaggca gcgatcgagg ccgaccgggc
      241 ccgggccagg ccatgatcct cgtcatcgac aactacgaca gcttcacctt caacctcgtc
      301 caggcgctcc aggccgccgg cgcggaggtc cacgtcgtgc gcaatgacgc gctggacatc
      361 gccgccgtcg aggccatggc cgacgatccg gatgcggacc tgcgcgggat cgtcatctcg
      421 cccggaccgg gcggcccggc cgacgccggg atctcggtcg gggtcgttcg ggtcgcggcg
      481 gaccgaggtc tgccactgct gggcgtctgt ctcgggatgc agtcgatggc ggcggcgttc
      541 ggggcctcga tcgtgcgggc gcccacgctc gtccacggcg aggcgtcgga ggtgacccac
      601 gacggtgccg ggctgctcga gggaatgccg cccgcgttca tggcggcgcg gtatcactcg
      661 ctcgtggtgg acgccgcgac cctgccgccg gagctgcgcg tgacggcgat gagcgaagtg
      721 gaccgggtca tcatggggat ccggcacgtc gccctgccgc tcgagggggt ccaattccac
      781 ccggagtccg tcctcacgcc ggagggcccg cacctgctgg cgaacttcct gcgccaggct
      841 ggcgaaggcg aggcgtccag gctggacgat gcgaccggat cgttcgccac ggccggcctc
      901 gccaggatgt cggatgagcc gctcggagag gcgacgcgat gagcgatctc gtgcgtgccg
      961 cgctcaatac gatcgtcgag ggcgggacgc tctcgatcga cgaagcccga gtcgcgatgg
     1021 gcgcggtcat ggacggcgag gcgacccccg cccagctggc ggccatgctg atgggcctgc
     1081 ggatgcgcgg cgagaccgtg gatgagctgg ccggcttcgc gacggcgatg cgcgagcggg
     1141 tcgtccacgt ggaggcgccc gagggcgcga tcgatgtcgt ggggaccggc ggcgatggga
     1201 gcggtacgtt caatatctcg acgactgcgg ccctcgtcgc ggctgccgcc ggggtcccgg
     1261 tcgccaagca cggcaaccgc gcgatcacct cgcgggccgg ctcggcggac gtcctggacg
     1321 cgctcggcat ccggatcgat cacgatgcga cctccgccgg cgcggcgctc cgcgagcttg
     1381 gattcgcgtt cctgttcgcg ccgaacttcc atccggcgat gaagcacgcc gggccgaccc
     1441 gccgggagat cggcgtccgc acggcattca acctgctcgg cccactgacc aacccggccg
     1501 ggacccgccg gcagctgctg ggcgtcggcg acgcggcggc ggccggccgg atggccgacg
     1561 tcgtgcaacg actgggcacg gacctgacct tcgtcatcca cggcgacggg gtcgacgagc
     1621 tgccgctcga cgggagcggg gtcctgtacg tcatcgcggg ggatacgatc gagcgccatg
     1681 ccatcgacgc ggccgccctt gggttcaagc gggccgcgac ggcccggctg tccgggggtt
     1741 cgccggccga gaacgcccgg atgaccgaga ccgtcctcca gggcgagcc
//
LOCUS       DATAIW010000018         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_553, whole genome shotgun sequence.
ACCESSION   DATAIW010000018 DATAIW010000000
VERSION     DATAIW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1212)
                     /locus_tag="VHM48_00625"
     CDS             complement(4..1212)
                     /locus_tag="VHM48_00625"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753932.1"
                     /translation="MTTRRGGRPSQVRPRPPSNGRPTPVKARPRPPAPGRLAAHRKIE
                     RGPGIALPFRLLAGVAVVALGVGVLLVANGGLGTVAEVVGNTFNGFVSDLTRTPRPSA
                     PELSAADPPTLEAPDEPYTNQPTVDLVGTVPAAVVGQADTRIRIYVAIGKGSPGVAIE
                     MAVPTSRTFRVPGLTLSPGTNTFTATIVGPGDLESEVSAAVSYVLDRTKPKVTITSPK
                     ANAIVNARSVQVVGVTQGRSTLSIHNLTTNATVTGAADAKGAFSIAVVIGTGTNNVQV
                     TATDPAGNVNIASVTLRRGSGALSANLTASFYQVRLSKLPESVQLSVLVNDPDGRPLQ
                     GASVTFTLAVPSVPAIASSVLTTSSTGRASFTTKIPKGATAGPFSVAVIVHTTDFGYT
                     TDRTVITLQK"
     gene            1421..1843
                     /locus_tag="VHM48_00630"
     CDS             1421..1843
                     /locus_tag="VHM48_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753933.1"
                     /translation="MSRPAVDSTTPPPRQDRRDNAAAGSAGAPVRAPMPIHPILPGRY
                     GAPTRSPLPAMPPSRGGFGLPNLRRNVCPHFYMTGSRGAIPIQAPHLLKALIRQDANV
                     SRCQLRGGVHLEQGYINDVCLTPRFNQCVFYEDDLTRE"
     gene            1840..>2779
                     /locus_tag="VHM48_00635"
     CDS             1840..>2779
                     /locus_tag="VHM48_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753934.1"
                     /translation="MTSEGGSDAAAIPTAAAPDDPGTRRAYEAALVALAERGRFGIRL
                     GLGRTRALLRELGDPQLAIRGALVAGTNGKGSVLALAGSALRAAGVRAGETPKPHLVS
                     YRERLQIAGRPVDAATFARLVATAIAAADRLPRRLGDPTEFELLTAVVFAWFAEERVD
                     LALVEVGLGGRLDATHAWDGGVAAITNVDLDHTDRLGPTIAHIAREKAAIIERGDLAV
                     TGAVGEARAIVRRRAARLGVPLAIVDPAPVVGVDRDGLIVELPRLGRTRVGLRGRHQA
                     ANVAVADAILDALTAAGIAAVPDAARRAGYADARWPG"
BASE COUNT          351 a          984 c         1037 g          407 t
ORIGIN      
        1 gacctacttc tggagcgtga tcacggtccg gtcggtcgtg tagccgaaat cggtcgtgtg
       61 gacgatcacc gcgaccgaga acgggcccgc ggtggcgccc ttcgggatct tcgtggtgaa
      121 cgatgcccgc ccggtgctcg acgtcgtcag caccgacgac gcgatcgcgg gcacgctcgg
      181 cacggcgagc gtgaacgtca cgctcgcgcc ctggaggggc cggccgtccg ggtcattgac
      241 gaggaccgac agctggaccg actcgggcag cttcgagagc ctgacctggt agaaggaggc
      301 ggtcaggttc gcgctgaggg cgccgctccc gcgccgtaac gtcaccgagg cgatgttgac
      361 gttgccggcc ggatcggtcg cggtcacctg gacgttgttc gtcccggtgc cgatgaccac
      421 ggcgatcgag aacgccccct tcgcgtcggc cgcgccggtg accgtggcgt tcgtcgtgag
      481 gttgtggatg ctgagggtgc tccggccctg cgtcacgccg acgacctgga cggacctggc
      541 attgacgatc gcgttcgcct tgggcgaggt gatggtgacc ttcggcttgg tccggtcgag
      601 gacgtagctg accgcagccg agacctcgga ctcgagatcg cccgggccga cgatcgtcgc
      661 ggtgaacgtg ttcgtgcccg ggctcagggt cagccccggc acccggaacg tgcgcgatgt
      721 ggggaccgcc atctcgatcg cgacgccggg gctgcccttc ccgatcgcga cgtagatccg
      781 gatccgggtg tcggcctggc cgaccacggc cgcgggcacg gtcccgacga gatcgaccgt
      841 cggctggttc gtgtacggct cgtcgggcgc ctcgagcgtc ggcgggtcgg cggcggacag
      901 ctcgggagcg ctcggtcggg gcgttcgggt gaggtcgctc acgaagccgt tgaacgtgtt
      961 gccgacgact tcggcgaccg ttccgaggcc accgttggcc acgagcagga cgccgacccc
     1021 gagggcaacg acggccacgc ccgccagcag ccggaatggc agggcgatcc ccggtccgcg
     1081 ctcgatcttg cgatgggccg cgaggcgtcc gggcgccggg ggccggggcc gggccttgac
     1141 cggcgtcggg cgtccgttgg acggaggccg cggtcgcacc tgggatggtc gcccgcctcg
     1201 ccgtgtcgtc atcggcgtcg attgtcgcat gcggcaaccg tcgggcgcgg ccgatgatcg
     1261 tttcgatgca gagcgttgta gtcttgcgcg gtccggcgcg cgggccgccc gtcttgctgc
     1321 gcgccgcatc gagggatgga tgcaggctcg agacccgaaa cgtgatctcc cacggggttc
     1381 gttgggccgc cccgcgttcg gcgtcggtcc gatcgcccgg gtgagccggc cggcggtcga
     1441 ttcgaccacg ccgccgccca ggcaggatcg tcgcgacaac gccgccgccg gatccgccgg
     1501 tgcgcctgtc cgggcgccca tgccgatcca cccgatcctg ccgggtcggt acggggcgcc
     1561 gacgcgttcg cccctcccgg cgatgccgcc gtcgcgcggt ggcttcgggc tgccgaacct
     1621 ccggcgaaac gtgtgcccgc acttctacat gaccgggtca cgcggcgcga tcccgatcca
     1681 ggcgccgcac ctgctcaagg cgctcatccg ccaggacgcc aacgtgtcgc gctgccagct
     1741 ccgcggcggg gtccacctcg agcagggtta catcaacgac gtctgcctga cgccgcggtt
     1801 caaccagtgc gtcttctacg aggacgacct gacccgggag tgacgagcga gggcggcagc
     1861 gacgcggcgg ccatccccac cgccgcggcg ccggacgatc cggggacacg gcgcgcctac
     1921 gaggcggcgc tggtcgcact cgccgagcga gggcggttcg ggatccgcct cggcctcgga
     1981 cggacgcggg cgcttctccg ggagctgggc gacccgcagc tcgccatccg cggagcactt
     2041 gtcgcgggga ccaacggcaa gggcagcgtt ctggccctcg ccggttccgc ccttcgagcg
     2101 gccggcgtgc gggccgggga gacgccgaag ccgcacctgg tgtcctatcg cgagcggctc
     2161 cagatcgccg gtcgacccgt cgacgcggcc acgttcgcgc ggctggtggc aaccgcgatc
     2221 gcggccgccg accggttgcc gcgacgcctc ggcgacccga ccgagttcga actcctgacc
     2281 gcggtcgtct tcgcctggtt cgccgaggag cgggtcgacc tcgccctcgt cgaggtcggg
     2341 ttgggcgggc gcctcgacgc gacccatgcg tgggacggcg gcgtagcggc gatcaccaac
     2401 gtcgatctcg accacaccga ccggctcggc ccgacgatcg cccacatcgc ccgcgagaag
     2461 gccgcgatca tcgagcgtgg cgacctggcc gtgaccggcg cggtcgggga ggcacgagcg
     2521 atcgtccggc ggcgggccgc gcggctgggc gttcccctcg ccatcgtcga tccggcgcct
     2581 gtcgtggggg tcgatcgcga cgggctgatc gtcgagctgc cgcgcctggg acgaacgcgg
     2641 gtcggactgc gcggccgcca tcaggccgcg aacgtggccg tcgccgacgc gatcctcgac
     2701 gccctgaccg cggcggggat cgctgccgtt cccgatgcgg cccgccgggc cgggtacgcc
     2761 gatgcgcgct ggccgggcc
//
LOCUS       DATAIW010000019         9544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149542, whole genome shotgun sequence.
ACCESSION   DATAIW010000019 DATAIW010000000
VERSION     DATAIW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149542"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..416)
                     /locus_tag="VHM48_00640"
     CDS             complement(9..416)
                     /locus_tag="VHM48_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2753935.1"
                     /translation="MPGGRRQPRERGSDVIRVLVVDDNAAVRHFMVTLLRRRGLSVIA
                     APTAPDAIHLASNTERLDLLVTDVDMPGGSGLALAARLAADRPGLLILVVSGDPTHAT
                     GLQPGARFLTKPFTIEEFDLEVGTLLAWQSHGP"
     gene            complement(769..2553)
                     /locus_tag="VHM48_00645"
     CDS             complement(769..2553)
                     /locus_tag="VHM48_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX2753936.1"
                     /translation="MPPRPPVAATQTGDAGLVAAGVRLADAPPAPTWWHEAVFYQIYP
                     RSFADGNGDGIGDFVGMTARLDYLADLGIDALWISPHYPSPLRDLGYDISDYLTVDPQ
                     YGSLDDFRGFLDGAHARGIRVILDLVLNHTSDQHPWFAESRLSATNTKRDWYLWRDGR
                     DGGPPNNWMSQFGGSAWQLDPATGQYYYHAFLVDQPDLNWRHPELREAMWQAVRFWLD
                     LGVDGFRLDAIGNLFKAPELPDHVSARSVFELWWAWQTATSDEERSATTAGLTELFRH
                     QLDQPEVHELMRELRRLVSAYPDRVLVGETDRVEFYGHDDELNLTFNFPLMQTDRLTA
                     SWIRRNQTRQWTELPPGAWPATTLGNHDTSRIRSRFSAGDDDLARARLAAAVVLTLPG
                     TPFLYYGEEIGMTDFLLEDVEQFRDAWGLWFHRSAEAELGLSAPEALVISSRFGRDKS
                     RTPMQWTNAANAGFSPEGAATWLPTNPNHRSGVNVAEQLTDAGSLLRFYRRLLRVRRR
                     TPALRRGDLTMLVGSDDVLAFVRRSLPDHQACLVVLNLSDRPQRLRLDVGTDALDTLF
                     STRSQRGVIERPTDLRMAAHEIYIAELE"
     gene            complement(2590..3405)
                     /locus_tag="VHM48_00650"
     CDS             complement(2590..3405)
                     /locus_tag="VHM48_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2753937.1"
                     /translation="MPIHLGVGIVMAIWLIPTVGLFVNSFRTVHDMGEAGWWTALFAP
                     AKFTLDSYKTILETRGFVDSIVNSLLITIPATILQVVIATFAGYAFAWMKFRGRDLFF
                     IVIVGLMVVPVQMTFIPVLKLFAATGLAGTFIAVWLAHTGYGLPFQTYLLRSYIGSLP
                     REVFESAEIDGANHATRFWRIVVPMSIPAIAANTIFVFLGVWNDLLIAVTFLGQSANK
                     PLTVLIGQQTTSLGGNWQVLTAAAVIQMILPLAVFLGLQRYFVRGISAGAVKG"
     gene            complement(3480..4445)
                     /locus_tag="VHM48_00655"
     CDS             complement(3480..4445)
                     /locus_tag="VHM48_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2753938.1"
                     /translation="MDSRIVTAIVAVVGMPILLVGYIQVGEAILARLPKGLARRMRPY
                     VWVFPAVAFATIFMVLPTINTIVISFQNVKGAWVGIDNYVYFFTNPETLGSIKNNLLW
                     LVLFTGFVVGFGLLISVLFDRVKYESIAKTAVFLPLPISAVAASIIWKFMYEFQPAGH
                     AQTGTLNAILGVPGFQPIAWLVNNTTNNFALIAIGVWTSTGFAMVIISAALKGINSEL
                     LEAARVDGANEVQVLRGIIIPLLMPTLTVIATTMIITALKVFDIVFVLTNGAYGTDVI
                     ARQLFARIANSDYGRAAAVGVVLLLAVTPILLLNVKRFREQEEIR"
     gene            complement(4530..5897)
                     /locus_tag="VHM48_00660"
     CDS             complement(4530..5897)
                     /locus_tag="VHM48_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753939.1"
                     /translation="MALKSTRRSRTLTAGAIAAIALMAGCGGSATPSPSAPPAASQPP
                     ASAGASQPAPSSGGQIGGQLSVWTAWGGDELKSFQAVLKPFADQTGIQVSLLTIRDQD
                     LQLSNNVAAGTSLPDIANPPNPQRYTEWAQKGIMKPLETFLDMNAYTSSTLPGLLLQD
                     PNYGFVDGKHYLEMVKSQLKGLVWYDPKVYTGQAPASWDDLLAVQPPSGAKLFCAAFE
                     SGDASGWPASDDLANILMRQAGEKTYVDWYSGKVKWTDPAIKTAYQTFGKMVADAKVY
                     GGTNYVLSTNFGKAGDGLFSNPPGCLFMEQATFIPSFFQQNDPSLKSGTDYNFFAHPK
                     FNDQYAGNVEGFFDTFVMYNDTPQARALMQYMVTDQAQQLWVDNGGTLAASKNITKYP
                     DVLGENSSKILQGAKNILLTAGDYMPADMQHAFWKSLLDFTNDQSKLDEILANLDQVQ
                     ASSYK"
     gene            6478..8100
                     /locus_tag="VHM48_00665"
     CDS             6478..8100
                     /locus_tag="VHM48_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2753940.1"
                     /translation="MTTALAGAPWWQRGVVYQIYPLSFQDGNGDGIGDLAGIRSRLDH
                     LVDLGADAIWLSPIHPSPMHDHGYDVADYEDVHPRFGTLAEFDAFVEAVHGRGLKLVL
                     DLVANHTSHLHPWFVESRSSRDNPKRDWYIWRDPAPGGGPPNNWVSYFGSAWTLDELS
                     GQYYFHQFRAEQPEVNYRNPDVAAAYRDIMRFWLRRGVDGFRADVIALMSKDDRFLDE
                     PDDPAYVATEMAPYFSRIHLYTRDQPAVHEIIRGMRRTLDEFPDRVLIGELDPTEHLM
                     EFYGAGDECHLPFNFKLILEPWDPVVHRRLIDEYDAALPAGAWPNWVIGNHDRDRFVD
                     RFGRRQGRVAQMLVLTLRGTPIYYYGDEIGMPNGAVPPDAWSRIPEIDPPDPKRNRAE
                     SPRTPMQWTAGPNAGFCPPGVVPWMPIAAGHETINVEVETRDPTSVLSLVRRLIELRR
                     TSPALAVGAYRSIDPGADGLLAYERRLADDRFLVVLNYLDEPLEVALPGLDGRWTVAL
                     STELDRPDEPADRPLRLRANEGVILRAAPVAT"
     gene            complement(8126..9196)
                     /locus_tag="VHM48_00670"
     CDS             complement(8126..9196)
                     /locus_tag="VHM48_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2753941.1"
                     /translation="MPPRSPTSRDVARLAAVSRTTVSYVLNNNNSTIAISPATRQRVL
                     DAARQLGYHPNAAARSLRRQMAGVVGLVTYDPAERVGGNAFLPLVMDGILSVLGPTGI
                     KLIVDAATPGQPDPCVTLVRGGHVDGLIVTGAQSNNEELRLLYDDGVPIVMWGKVSGN
                     ALPFVDIDNVAAARTATEHLISLGHTRIACITNASPAIDMAAADRLAGYRSALESHRI
                     TADPRLIRHANFSESSGFDAMRELLTLEERPTAAFVASDEVAIGAVKAAKVHGVNVPA
                     LLAVVGFDDLPYAPFVDPSLTTIRVPARDIGSEAARRVIAALGNGRRPPPVEERDGLP
                     RDASAVLETELVIRESSGPFLG"
     gene            9275..>9544
                     /locus_tag="VHM48_00675"
     CDS             9275..>9544
                     /locus_tag="VHM48_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753942.1"
                     /translation="MGKEDDGGSLDGSPLINDVDYEVTYGTDSFMVVNFDIIGRWHEK
                     GVRRGGLAPGASNDLDGQVSAPCDRRAATDLRRGHGQVSLQVPIVY"
BASE COUNT         1573 a         3087 c         3326 g         1558 t
ORIGIN      
        1 agcgagcttc agggcccgtg tgactgccag gcgaggagcg tccccacctc gaggtcgaac
       61 tcctcgatcg tgaagggctt ggttaggaag cgcgcaccgg gctgaagccc cgtggcatgg
      121 gtcgggtcgc ccgagaccac gaggatcaag agacccggcc ggtcggccgc cagccgcgcc
      181 gcgagggcaa gaccggaccc acccggcatg tcgacgtcgg tgacgagaag gtcaagtcgc
      241 tcggtgttcg aggccaggtg gatagcgtcc ggcgcggttg gtgccgcgat cacgctgagg
      301 ccccgccgac gcaacagcgt caccatgaaa tggcggacgg cggcgttgtc atcgacgacg
      361 agcactcgga tgacgtccga gccacgctcg cgcggttggc gacgaccccc gggcacggcc
      421 gaaggctcat ggcggaaccg cgctcccccc gaatggcccc caccctttcg tttgatcatc
      481 cggctgcaac ttcccgcacc ggctactgtc cgaggagggc atcgatcgtg gcggccgcct
      541 gcggacgtgc gaaatgatcg ccctggccga gatcacagcc cactcgcttc acgagggcgg
      601 cccgggccgg tagctcaacg ccttccgcga ttgggatggc atcagtgacc tctcgagcgg
      661 atctgcccgt acgcgagcgc gaacgaccca tgggcttggc caggacaggg aacggcccaa
      721 ctccggcgtc cgccgagcga cgagcgatct gcgcgccatg cacgaccgtc actcgagctc
      781 ggcgatgtag atctcgtgag ccgccatccg gaggtcggtc ggccgctcga tcacacctcg
      841 ctgggagcgg gttgagaaca acgtgtcgag cgcgtcggtc ccgacgtcga gccggagccg
      901 ctgcggtcga tcggacaggt tcaggacgac caggcacgcc tggtgatcgg gcaggctgcg
      961 ccggacgaag gcgaggacgt cgtcggaccc aacgagcatc gtcagatcgc cccggcgcag
     1021 cgctggcgtg cgtcgccgga cgcggagcag gcgccggtag aaccggagga gggagccggc
     1081 gtcggtcagc tgttcggcga cgttgacgcc cgaccggtgg ttcgggttcg tcgggagcca
     1141 ggtggcggct ccctccgggc tgaacccagc gttcgccgcg ttcgtccact gcatcggcgt
     1201 gcggctcttg tcccgcccga agcgcgagct gatcaccagc gcctcggggg cgctgaggcc
     1261 gagctcggcc tcggcggagc gatggaacca gagcccccac gcgtcacgga actgctcgac
     1321 gtcctcgagg aggaaatcgg tcatcccgat ctcctcgccg tagtacagga acggcgtccc
     1381 gggcagggtc agcacgacgg ccgccgccag acgggccctg gcgaggtcgt cgtcccccgc
     1441 gctgaaccgc gaccggatcc gggaggtgtc gtggttaccc agggtggtgg ccggccatgc
     1501 ccccggcggc aactcagtcc actggcgcgt ctggttgcgc cggatccagg acgcggtcag
     1561 ccgatccgtc tgcatgagcg ggaagttgaa ggtcaggttc agctcgtcgt cgtgcccgta
     1621 gaactccact cggtcagtct cgccgacgag gacgcggtcc ggataggcgc tcacgaggcg
     1681 acgcagctcg cgcatcagct cgtgaacttc gggctggtcg agctggtgtc ggaagagctc
     1741 ggtcagccct gccgttgtcg cggaccgttc ctcgtcgctc gtcgcggtct gccacgccca
     1801 ccacagctcg aataccgagc gggccgagac gtgatcgggg agttcaggcg ccttgaacag
     1861 gttaccgatg gcgtcgaggc ggaacccgtc cacgccgagg tcgagccaga accggacggc
     1921 ctgccacatc gcctcgcgga gctcgggatg acgccagttg aggtcgggct gatcgacgag
     1981 gaacgcgtgg tagtaatact ggccggtcgc cggatcgagt tgccatgccg acccgccgaa
     2041 ctgggacatc cagttgttgg gcggcccacc gtcccgaccg tcgcgccaga ggtaccagtc
     2101 gcgcttcgtg ttcgtcgcgc tcaagcgcga ttcggcgaac cacggatgct ggtcggacgt
     2161 gtggttcaag acgaggtcga ggatgacccg gatgccgcgc gcgtgggccc cgtcgaggaa
     2221 gccgcggaag tcgtcgagcg agccgtattg cggatcgacg gtcaggtagt cgctgatgtc
     2281 gtaaccgagg tcgcggaggg gcgacgggta gtgtggcgag atccagagcg catcgatccc
     2341 cagatcggcc aggtagtcga gccgcgcggt catgccgacg aagtcgccga tcccgtcgcc
     2401 gttgccgtcc gcgaagctgc gcgggtagat ctggtagaag accgcctcgt gccaccacgt
     2461 cggcgcggga ggggcgtcgg cgagccggac cccggccgcg acgaggccag catcacccgt
     2521 ctgcgtcgcg gcgaccgggg ggcgcggtgg cacggctggt caggtccggt gggtgaggtc
     2581 gggcgccggt cagcctttga cggcgccggc gctgatccct cggacgaagt agcgctgcag
     2641 gccgaggaac accgccagcg gcaggatcat ctggatgacg gccgcggccg tcaggacctg
     2701 ccagttcccg ccgagcgacg tcgtctgctg accgatcagc accgtgagcg gcttgtttgc
     2761 gctctgacca aggaaggtga ccgcgatgag caggtcgttc cacacaccca ggaacacgaa
     2821 gatcgtgttc gccgcgatgg ccgggatgct catcgggacc acgatccgcc agaacctggt
     2881 cgcgtgattc gccccgtcga tctcggccga ctcgaacacc tccctgggca ggctgccgat
     2941 gtagctgcgc aggaggtagg tctggaacgg caggccatac ccggtgtggg ccagccagac
     3001 ggcgatgaag gtgcccgcga ggcccgtcgc cgcgaagagc ttgaggaccg ggatgaaggt
     3061 catctggacc gggacgacca tcaggccgac gatcacgatg aagaacaggt cccggccgcg
     3121 aaacttcatc caggcgaatg cgtagcccgc gaacgtcgcg atcacgacct ggaggatcgt
     3181 tgccgggatc gtgatcaaca ggctgttcac gatgctgtcg acgaagccgc gggtctcgag
     3241 gatggtcttg tagctgtcga gcgtgaactt ggccggcgcg aacagggccg tccaccagcc
     3301 ggcttcgccc atgtcgtgga ccgtccggaa cgagttgacg aagaggccga ccgtggggat
     3361 gagccagatc gccatgacga tgccgacgcc gaggtggatc ggcaccctgc ggaagcgtcg
     3421 tcgcaacgca ctggaggcgg ggcgagcacg agcgtcgtcg ggatcccgct cgatcgcggt
     3481 catcggatct cctcctgctc gcggaagcgc ttgacgttga gcagcaggat cggcgtcacc
     3541 gcgagcagca gcacgacgcc gacggccgcc gcccggccgt agtcggaatt ggcgatgcgg
     3601 gcgaacagct ggcgggcgat cacgtccgtg ccgtacgcgc cgttcgtcag gacgaagacg
     3661 atgtcgaaga ccttcagggc cgtgatgatc atcgtcgtcg cgatgacggt gagggtcggc
     3721 atcaggaggg ggatgatgat cccgcgcagg acctggacct cgttcgcccc gtcaacacgg
     3781 gctgcctcga gcagttcgga attgatgccc ttcagggccg ccgagatgat caccatcgcg
     3841 aacccggtcg aggtccagac tccgatcgcg atcagcgcga agttgttggt cgtgttgttg
     3901 acgagccagg cgatcggttg gaagcccggc acgccgagaa tcgcgttgag cgtcccggtc
     3961 tgggcatggc cggcgggctg gaattcgtac atgaacttcc agatgatcga cgctgcgacc
     4021 gcgctgatcg ggagcggcag gaagacggcc gtcttggcga tcgattcgta cttgacgcgg
     4081 tcgaagagga ccgagatcag cagcccgaag ccaacgacga agccggtgaa caacaccagc
     4141 cacaggaggt tgttcttgat cgagccaagc gtctcgggat tcgtgaagaa gtagacgtag
     4201 ttgtcgatgc cgacccaggc gcccttgacg ttctggaagc tgatgacgat cgtattgatg
     4261 gtcggcagga ccatgaagat cgtcgcgaag gcgacggccg ggaacaccca cacgtatggc
     4321 cgcatccgac gggcgagtcc cttcggcagg cgggccagga tcgcctcccc gacctggatg
     4381 tagccgacca gcaggatcgg cattccaacg accgccacga tcgcagtgac gatccgactg
     4441 tccatgcggc cctccctccg gggaagacgt cggtcggggc gggaggagtg ccgccccgac
     4501 cgactcgggt gatgggggct ggtgggacct tacttgtacg agctggcctg aacctggtcg
     4561 aggttggcca ggatctcgtc aagcttggac tgatcgttcg tgaagtcgag gagcgacttc
     4621 cagaaggcgt gctgcatgtc ggccggcatg tagtcaccgg cagtcagcag gatgttcttc
     4681 gcaccctgga ggatcttcga cgaattctcg ccgagcacgt ccgggtactt ggtgatgttc
     4741 ttgctcgcgg cgagcgtgcc gccattgtcg acccagagct gctgcgcctg gtcagtcacc
     4801 atgtactgca tcagcgctcg tgcctggggc gtgtcgttgt acatcacgaa ggtgtcgaag
     4861 aagccctcga cgttccctgc gtactggtcg ttgaacttgg gatgggcgaa gaagttgtag
     4921 tcggtgcccg acttcaggct ggggtcgttc tgctggaaga agctcggaat gaacgtcgcc
     4981 tgctccatga acaggcagcc cggtgggttg ctgaagagcc cgtcaccggc cttgccgaag
     5041 ttggtggaca ggacgtagtt cgtgccaccg tagaccttgg cgtcggcgac catcttgccg
     5101 aaggtctggt acgccgtctt gatggctgga tcggtccact tgaccttgcc gctgtaccag
     5161 tcgacgtacg tcttctcgcc ggcctggcgc atgaggatgt tggccaggtc gtcggacgcc
     5221 ggccaacccg acgcgtcgcc cgattcgaac gcggcgcaga agagcttcgc gccggacggc
     5281 ggctggaccg cgaggaggtc gtcccacgac gcgggggcct ggccggtgta gaccttcggg
     5341 tcgtaccaaa ctagaccctt cagctgcgac ttgaccatct cgaggtagtg cttgccgtcg
     5401 acgaacccgt agttcgggtc ctggagcagc aagccaggca gcgtgctgct cgtgtaggcg
     5461 ttcatgtcga ggaaggtctc gaggggcttc atgatcccct tctgggccca ctcggtatag
     5521 cgctgcgggt tcggtgggtt ggcgatgtcg ggcaggctgg tgccggccgc gacgttgttg
     5581 ctcagctgca gatcctggtc gcggatcgtc agcaggctga cctggatacc ggtctggtcc
     5641 gcgaacggct tgagcacggc ctggaagctc ttgagctcgt cgccgcccca ggccgtccag
     5701 acgctgagct ggccgccgat ctggccgccg ctactcggcg cgggctggct cgctccagcc
     5761 gacgccggtg gctggctcgc cgctggcggg gccgacgggc ttggtgtggc cgagccgccg
     5821 cagccggcca tcaaggcgat cgcggcgatg gcgccggcgg tcagggtacg cgaccttcgc
     5881 gtggatttca gtgccacagg tgtattcctc ctccgctcga accaattggg tggatggacg
     5941 ccctcgcgtc cgtgctcttg agcgaacctc cttgagctgg ggtgactccg accggctcgt
     6001 gggctcaatc ggaggccggt cgcaggggac gccgatcgct ggggatatga gtcggcggcc
     6061 gggaccgggg ccggccatca gcccgccacc gggcgcggag cgccctggtg gggcgggatg
     6121 cctccatagc cttgacccgt gcgctccttc ggaccccgat cccgacgggg gtccgacgag
     6181 cgattctggt cgccgcgggg gacgccgcgc gataaggccc cgcccgcggc gtcgactcgg
     6241 atagcaggaa ttcgcgaccg gccatgagcc ctactcccca acggcagcga tccgacactc
     6301 tacacgtgtc acgcggatcg tggcagcgtt gatccgtggc gtcaagaacc agtttttctc
     6361 gcacccgatg ggcaagcgtc ttgacgccgc gcgttcctca ttgtcacgat acgggtgaca
     6421 cgttccgccc acgcggctac gggccgcctt ctcgatcgtg caaggagcag ccaagacatg
     6481 acgacggcgt tggcaggcgc cccctggtgg cagcgcgggg tcgtctacca gatctatccg
     6541 ctcagcttcc aggacggcaa cggcgacggc atcggcgacc ttgccgggat ccggagtcgc
     6601 ctcgatcacc tcgtcgatct tggagccgac gcgatctggc tctcgccgat ccacccctcg
     6661 ccgatgcacg atcatggcta cgacgtcgcc gactacgagg acgttcatcc caggttcggc
     6721 acgttggccg agttcgatgc cttcgtcgag gcggtccacg gccgcgggct gaagctcgtg
     6781 ctggatctcg tcgcgaacca cacgtcgcac ctccacccgt ggttcgtcga gagccgctcg
     6841 tcgcgcgaca acccgaagcg cgactggtac atctggcgcg atcccgcgcc gggcggcggg
     6901 ccgccgaaca actgggtgag ctacttcggc tcggcctgga ccctggacga gctgagcggg
     6961 cagtactact tccaccagtt tcgtgcggag cagccggagg tcaactaccg caacccagac
     7021 gtggcggcgg cctatcgcga catcatgcgg ttctggctcc gacggggcgt cgacggcttt
     7081 cgggcggacg tcatcgccct gatgtcgaag gacgaccgat tcctcgacga gccggacgac
     7141 ccggcctatg tcgcgaccga gatggcgccg tacttcagcc ggatccacct ctacacacgg
     7201 gaccagcccg cggtccacga gatcatccgc ggcatgcgcc ggaccctcga cgagtttccg
     7261 gatcgggtgc tgatcggtga gctcgacccc accgagcacc tgatggagtt ctacggtgcg
     7321 ggcgacgagt gccacctgcc gttcaacttc aagctgattc tcgagccgtg ggatcccgtc
     7381 gtccaccgcc gcttgatcga cgagtacgac gccgccctgc cggccggcgc ctggccgaac
     7441 tgggtgatcg ggaaccatga tcgcgaccgc ttcgtggacc gcttcgggcg acggcagggt
     7501 cgggtggccc agatgctggt gctcaccctc cgcggcacgc cgatctacta ctacggggac
     7561 gagatcggca tgcccaacgg cgcggtgccg ccggacgcgt ggagcagaat ccccgagatc
     7621 gatccgcccg acccgaagcg gaatcgcgcc gagtcgccgc ggacgccgat gcagtggacc
     7681 gcgggtccga acgccggctt ctgtccgccg ggcgtcgtgc cctggatgcc gatcgccgcc
     7741 ggccacgaaa cgatcaacgt ggaggtcgaa acccgcgatc cgacgtcggt cctgtcgctc
     7801 gttcggcgac tgatcgagct gcgtcggacg tcgccggccc tcgccgtggg tgcataccgc
     7861 tcgatcgacc ccggtgcgga cggcttgctc gcctacgagc ggcggctggc ggacgaccgg
     7921 ttcctcgtcg tcctcaatta cctcgacgag ccgctggagg tcgctctccc agggctcgac
     7981 ggtcgctgga cggtcgccct ctcgaccgag ctcgatcgcc ccgatgagcc cgccgaccgc
     8041 ccactccggc tccgggcgaa cgagggcgtc atcctgcggg ccgccccggt cgccacgtga
     8101 tggccgcctt ggagatcggc gccgctcagc cgaggaacgg gccgctcgac tcgcggatga
     8161 cgagctcggt ctcgaggacc gccgaggcgt cgcgggggag cccgtctctc tcttcgactg
     8221 gcggcggacg acgaccgttt cccagggcgg cgatcacccg ccgggcggcc tcggagccga
     8281 tgtcgcgagc aggtacccgg atcgtcgtca acgacggatc gacgaatggc gcatacggca
     8341 ggtcgtcgaa gccaaccact gccaggagcg cggggacgtt cacgccgtgg accttggcgg
     8401 ccttgacggc gccgatcgcg acttcgtcgc tggcgacgaa ggctgccgtc gggcgctcct
     8461 cgagcgtcaa cagttcgcgc atcgcatcaa acccgctgct ctcgctgaaa ttcgcgtggc
     8521 ggatcaggcg cgggtcagca gtgatgcggt gactctcgag ggcagatcgg tagccggcga
     8581 gccgatccgc ggcggccatg tcgatcgcgg gcgaggcatt cgtgatgcac gcgatccggg
     8641 tgtggcccag ggaaatcaga tgctcggtcg cggtgcgggc cgcggcgacg ttgtcgatgt
     8701 cgacgaacgg gagcgcgttg cccgagacct tgccccacat cacgatcggc acgccgtcgt
     8761 cgtagaggag ccggagctcc tcgttgttcg attgcgcgcc ggtcacgatc aacccgtcca
     8821 cgtggccacc ccggacgagg gtcacgcacg gatcgggctg accgggggtc gccgcgtcca
     8881 cgatgagctt gattcccgtt ggccccagca ccgacaggat cccatccatg acgagcggca
     8941 ggaaggcgtt cccgccgacg cgctcggccg ggtcataggt caccaacccg acgacgccgg
     9001 ccatctggcg gcggagcgag cgcgccgcgg cgttcgggtg gtagcccagc tggcgggccg
     9061 cgtcgaggac tcgctggcgg gtcgccggac tgatcgcgat cgtggagttg ttattgttga
     9121 ggacgtacga gaccgtggtt ctcgagaccg cggccaaacg agcgacatcg cggctcgttg
     9181 gacttcgcgg gggcatccgc tacctccagt gacacgtgtc cgttgatgga ttgtaaccgc
     9241 tcgatcgccg gcgaggccgt caggtgcctc cgagatggga aaggaggacg acggtggctc
     9301 cctcgacggc agcccgctga tcaacgacgt cgactacgag gtcacatacg gcaccgacag
     9361 cttcatggtc gtgaacttcg acatcatcgg ccggtggcat gagaagggcg ttcgccgcgg
     9421 aggcctcgcg ccgggtgcat cgaacgatct cgatggccaa gtttcggcgc cgtgtgaccg
     9481 acgggcagcg accgatctcc gccggggaca cggacaggtg agcctacagg tgccgatcgt
     9541 ctac
//
LOCUS       DATAIW010000020        13440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_578, whole genome shotgun sequence.
ACCESSION   DATAIW010000020 DATAIW010000000
VERSION     DATAIW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..457
                     /locus_tag="VHM48_00680"
     CDS             11..457
                     /locus_tag="VHM48_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2753943.1"
                     /translation="MRRRRVRTLVRKESSLRARLRRVLAPNREALLPLVRPDFELRSE
                     LGPVWPGIIQRLERALDGLENARELLVGSFDLYLGRASQRTNEVMKALTLLSSIALPG
                     VVVAGVMGMNFKAPIFDDPNNFLVVIAAMLTFSVSILGVARWRHWV"
     gene            complement(476..4405)
                     /locus_tag="VHM48_00685"
     CDS             complement(476..4405)
                     /locus_tag="VHM48_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2753944.1"
                     /translation="MEVTAEVASGGVDGGPHPPLGPGPLPVDLRAILQGIAAGVTVQD
                     EGGRLLFANDDAARLSGFGSSAEMLAATPDELVERLELTHEDGRPFDRSLLPGRRVLA
                     GEPVDPVIIGFSPVAGGDIRWSMLLARAATLGDGRRVAINTFHDVTSRIETERRIRAS
                     ERSTQEIAEGRRRAEEIARMLADAALRLDEAADLEAIIQVAAMVAIPVVADWCVVDLV
                     LADGTVRRAALAARDERLRELVERLRDQPSVGSPVRGSGRALASGSAVVALDIEAWWA
                     TETPPDPELREMLEATGIRSAIAQPLIARGDVIGSIVYATVDDRSFDQPAVAATLEIA
                     NRIALAIANGRSHEAEQLARRAAEELADRMERLQTVTRTLADATTIADVAAVVADEAR
                     QALGAESVVVGLLDETATRLDLVVRESGRPPDENDGQPPGGAARSLSLDADVPLATAV
                     RKVRPVWVDGRSKDGVEASGCAVPLVGDHAPLGGLWLGFREPRTFAPADQRLVRAYAD
                     LAAGAIVRLRLGSVRQLLLSANEAERARLESVLRRMPIGVILAAVPDGRFLYANEAAQ
                     RLSPLPVELGETPAYEAARGFRPDGSELGPEDWPLRRAMRGETVENEVIEIAYDGGSR
                     RTYGLSAAPIPGPTGAIETAVVTYADVTDRIQAQQRETFLARASEVLASSLDYEATVQ
                     AVADLAVPDFADWCVVQLASEDGLPRRIAVAHRDPDLVALAVRLQEEYPADPDATSGV
                     AEILRTGKSEYVAEIPDEVLDAAARDERHLEMIRALALRSYISVPLIAAGRTLGVLTL
                     VASDSGRRFEPDDVAFAENLAVRAAGAIENARLFREGVRFKRLLDATGDAILMLDPAA
                     GRIAYANRGAAEQLERSVDDLVGSPIGEHLDATGTAALIEAMSDLTGGTTDARTVTLQ
                     LHRPSGSIRPVEVRLEYVAPDGAPARILAIARDIRDRIQAQERLRGLAAAEHARAAEL
                     NAVIRAMGDGVVVCDRAGRIILANPAAENVFPDVDETTYVEILAQLDDPDGLAPELGG
                     RGGPVELRVRSEDERWIEVSTWPVAAGPDRLVGHEDETIVLLRDVTVQRQQQAVRDTF
                     IGVLSHELRTPVTTIYAGAKVLARPGTMPDATRQEIFHDIVIESERLHRLVEDVVAMT
                     RFGDEGGDVGAEPVLLQRLLPAVVASEDGRWPGVSFEAVVPPGLPTVIADPTYVEQVV
                     RNLLSNAAKYGGPGASVRALVEAADGEVLVRILDDGPGFPPDERERLFDLFFRSAQTA
                     RAAAGAGIGLFVCARLIKAMGGRIWATNRSEGGAEFGFALRVMGEDD"
     gene            complement(4551..5711)
                     /locus_tag="VHM48_00690"
     CDS             complement(4551..5711)
                     /locus_tag="VHM48_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2753945.1"
                     /translation="MTLDDGFGRSVTRGAGLAVGVGLVAGLVLLGIAAGHVLILMLVA
                     VILASGLQPFVAWIRGHVPIGRGPTILLVYGAFLAVVVGMALIVLPVAIAQLERTMAS
                     LPPFFQRAREWATTISPPGLSRSVTTLIDSASRALQPPPNAAPAPGQVVQVGLTLAET
                     VTSLLTLLTVVYFWLTEHARLQRYALAFVPADRRKRARDVWNAAETRLGMWVRGELIL
                     MATIGVATAVTYTLLGVPAALLLGLIAAIAEAIPIVGPLLGAIPAVLVAATVSPQLAL
                     AVGGAYLVLQLIEGNFLVPLVMRNTVGISPFLVILSVLIGGAAGGFIGALLAVPIAAV
                     GEVLIEGLQAREIPVAQGPTATETTADESNAADEPDAGTGSRGAARGTPASP"
     gene            6076..6951
                     /gene="efeU"
                     /locus_tag="VHM48_00695"
     CDS             6076..6951
                     /gene="efeU"
                     /locus_tag="VHM48_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEX2753946.1"
                     /translation="MDLGALVSGLLTGLREGVEAALIVSIVITYLVRSGRPEQIGRVW
                     IGTGLAAVISLVVGIVIYSTVGAFEAPYEQIFEGTTLLVAAVVVTWMLFWMRRQARSV
                     KSELQAAVDRTIAGGASWGLAALAFTAVIREGLETSIFLVGQAASNRTEAGWILVGAF
                     AGLAIAILIGYGFYRGSSRLNLASFFRWTGIGLVFIAAGLLSHGIGEFIEIGALGNGP
                     WTATAFDISGVLSDETGVGSFLRAIFGYSAAPAVLTLAAHVGYLVVILALFLRPARRP
                     LPPAPSAPETVTARS"
     gene            complement(7074..8366)
                     /locus_tag="VHM48_00700"
     CDS             complement(7074..8366)
                     /locus_tag="VHM48_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2753947.1"
                     /translation="MLPSTGPAPARASGPAVRLVLVALVVIAAAVLIALLASRSAPRG
                     GGTAGGPTGLPPGSSRSGGSGFDPTRVAITLEPFADGLTAPLAIANAGDGSGRLFVAE
                     QGGRIRIIRNGRVEATPFLDISDEITSGGERGLLGLAFHPDYPADGRIFVDYTDTNGD
                     TQVSSFEVSGGDPDRVDPTSEMRILHVKQPFANHNGGALVFDPGGMLLISLGDGGSGG
                     DPQGNGQSLETLLGKILRIDVAQSDADHPYRIPADNPFRDGADRHRPEIWLWGLRNPW
                     RMSFDRATGDLWIGDVGQNAWEEVDVQRAGTAPGTNFGWNRMEGTHCFQPEQGCEGVA
                     LTLPVTDYGHDLGCTVIGGGVYRGSVQTALLGGYVFGDYCSGRVWAIDPAGGGYRAPV
                     LAGETGRNPSAFGEDEAGELFVADIGGGAILRVAATAR"
     gene            8424..8756
                     /locus_tag="VHM48_00705"
     CDS             8424..8756
                     /locus_tag="VHM48_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2753948.1"
                     /translation="MDAFELADLGARREASGALYLEFLSVPDLSVGLYVLAAGQPDLQ
                     QPHTEDEVYYVVSGRGRITVGDEVRDVRPGSIVFVGTAVSHRFHDITEDLTLLVVFGP
                     AKGSRASG"
     gene            complement(9006..9776)
                     /locus_tag="VHM48_00710"
     CDS             complement(9006..9776)
                     /locus_tag="VHM48_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2753949.1"
                     /translation="MLARRGLSRIVAVGRLRTGTSGFAYPAWSPRFYPPGARGPALLA
                     HYASRLATVELNNTFYRQPSPAAVDGWLAATPDDFRFAVKAQRGSSFRALQVDPAASV
                     DWLTTPLRRFGERLGTILFRVPDGVTRSDERLAGLLATWPADLPLALEFQDPSWHVDE
                     VFGAARDAGAAIVTTDLDTDPEPPSIRRSGPFLYLRLRRTDYPADELARWLDRLEPFL
                     ADGVDAYVYFRHDEVGRGAELALELAALGDAAGFGVAR"
     gene            complement(9755..10315)
                     /locus_tag="VHM48_00715"
     CDS             complement(9755..10315)
                     /locus_tag="VHM48_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX2753950.1"
                     /translation="MPTPSVSPRTQPAAKARIAGPATAAVVADEPGIGAWRSFLTAHA
                     RITRRLDEELQVAHGLSLAEYDALLQIAHAPGRRVRMNVLAERVILSRSGITRLVDRL
                     EAAGSVERAACTTDARGQEAVLTPLGLERLRSAATTHLDGVRRYFLGRLDAADLAGLE
                     ASLGRVADPLASGNHPTHEGCSPAEA"
     gene            10454..11032
                     /locus_tag="VHM48_00720"
     CDS             10454..11032
                     /locus_tag="VHM48_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2753951.1"
                     /translation="MSTTTWKLDPAHTDITFSARHMMITTVRGTFADVDGTLAIDDAD
                     PTAANGEIRVQAASISTGFGARDTHLRSADFFDVETWPEIVVRFDGVEPVKGDDYRVT
                     ADLTIRAITRPVTFEVEHVGETTNFQGTRHIAFTAQATINREDWGLTWNMSLETGGWL
                     VGKDIKLVVEVVADEVVLAPAEGERELVRAAA"
     gene            11144..12424
                     /locus_tag="VHM48_00725"
     CDS             11144..12424
                     /locus_tag="VHM48_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX2753952.1"
                     /translation="MTTFDVEALRRRFPALAIEDGGRPVALFDGPGGTQVPESVIAAI
                     GDYYRTSNANHDGPFLTSRRSDESVHEAHQAMADMLGSASADEIKFGANMTSLTFHVS
                     RSIGATMAPGDEIVVTILDHEGNVGPWKAIAADRGLVVRTVDIRVSDVTLDLATLDAV
                     LGPRTKLVAVGYASNAVGTINPVAEIVRRAHAVGAWTYVDAVHAAPHLPIDVRAIGTD
                     FLACSTYKFFGPHAGVLYGRAEILESLPPYKLRPATDRFETGTGNFEGLAGVTAAVEY
                     LADIGSAYGGASGVAARRERVVAGMTAIRAYELDLYRRLAGGLEAIPGLTMYGITDRA
                     RFDERTPTAALRLAGVAPRDVAEALGAEGIAVWDGDFYATGLIERLGLAESGGVVRIG
                     LTHYNTAAEVDRLVAALGRIAAAAGARATAAASA"
     gene            12467..>13440
                     /locus_tag="VHM48_00730"
     CDS             12467..>13440
                     /locus_tag="VHM48_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2753953.1"
                     /translation="MPAVDVVVIGGGIVGTAIAAFLAEAGLRVRLHEQETIAAGASGR
                     NSGIVQHPFDPVMAGLYRGTLAEYRRLADDSAASFRLDDEPAGLLYVGRDRAAAERTA
                     ATWAEAWPASAPEMLDGVALTRLEPALAPDLVACRLAIGFPVAPAAATEAFASQARRL
                     GVEIIVGGGPARPAIGGDRVRGIEWGGRIEPAGVVVVAAGPWTPDVIDPGGSWRPIRS
                     SWGVVASVVVANAPRHGLEAIDIDIEPSEGDGRAPDHAGPDEAIEFSLVPAAGSSALG
                     STFLPDEPDPDAWLDALRRVGSRYVPGVADAPLLGLRRCARPVSRDGR"
BASE COUNT         1906 a         4811 c         4853 g         1870 t
ORIGIN      
        1 tagccgggcg atccgccggc ggagggtcag aaccctcgtc aggaaggagt cctcgcttcg
       61 agcccggcta cgccgcgtgc tggcccccaa tcgcgaggcc ctcctcccgc tcgtgcggcc
      121 ggacttcgag ctccgttcgg agctcggtcc cgtctggccc gggatcatcc agcggctcga
      181 gcgcgcgctc gacgggttgg agaacgcccg cgagctgctc gtcggctcgt tcgacctcta
      241 cctgggtcgc gcctcgcaac ggaccaacga ggtgatgaag gcgctgacgc tcctgtcctc
      301 gatcgccctg ccgggggtgg tcgtcgcggg agtcatgggg atgaacttca aggcgccgat
      361 cttcgacgat ccgaacaact tcctggtcgt catcgcggcg atgctcacgt tctccgtgtc
      421 gatcctcggc gtcgctcgct ggcgccattg ggtctgacgc ccgcggagat cgacctcagt
      481 cgtcctcgcc catgacgcgc agggcgaacc cgaactcggc cccgccctcc gagcggttcg
      541 tcgcccagat ccggccgccc atggccttga tcagccgggc gcagacgaac agtccaatgc
      601 ctgcaccggc cgcggcccgt gcggtctgcg cggagcggaa gaacaagtcg aagagacgtt
      661 ccctctcgtc gggtgggaag cccggaccgt catcgaggat ccggaccagg acctcgccat
      721 cggcggcctc caccagggcc cgcacggacg cccccggccc accgtatttc gccgcgttcg
      781 acaacaggtt gcgaacgacc tgctcgacgt aggtcggatc ggcgatcacg gtcgggaggc
      841 cgggcggaac gacggcctcg aaggacaccc ccggccagcg gccgtcctcg gaggcgacca
      901 cagccggcag gagccgctgg aggaggacgg gctcggcccc gacatcgccg ccctcgtcgc
      961 cgaagcgggt catcgcgacg acgtcctcga cgagacggtg gaggcgctcc gactcgatga
     1021 cgatgtcgtg gaagatctcc tggcgcgtcg cgtccggcat ggtccccggt cgggcgagga
     1081 ccttcgcccc cgcgtagatc gtcgtcaccg gggtccgcag ctcgtgtgac aggacgccga
     1141 tgaacgtgtc ccggacggcc tgctgctggc gctgcacggt gacgtcgcgg aggaggacga
     1201 tcgtctcgtc ctcatgaccg accaatcggt ccggaccggc cgcgacgggc cacgtgctga
     1261 cctcgatcca gcgctcatcc tcggaccgga cccggagctc gaccggaccg cctcgcccgc
     1321 ccagctccgg agccaggccg tcggggtcgt cgagctgggc gaggatctcc acgtacgtcg
     1381 tctcatcgac gtcggggaag acattctcgg cggccgggtt ggcgaggatg atccggccgg
     1441 cccggtcgca gacgacgacg ccgtcgccca tcgctcggat cacggcattg agctcggccg
     1501 cgcgggcatg ctcggccgcc gccagtccgc ggaggcgctc ctgcgcctgg atccggtcgc
     1561 ggatgtcgcg ggcgatcgcg aggatccggg cgggcgcacc gtcgggcgcg acgtattcga
     1621 ggcggacctc caccggccgg atggagccgc tcggccggtg gagttggagc gtcaccgtcc
     1681 gggcgtcggt cgtgccgccg gtcaggtcgc tcatcgcctc gatgagggcg gcggttccgg
     1741 tcgcgtcgag gtgctcgccg atcggcgagc cgacgagatc gtcgaccgat cgctcgagct
     1801 gctctgcggc gccacggttg gcgtaggcga tccgccccgc ggccgggtcg agcatcagga
     1861 tggcgtcgcc cgtcgcgtcg aggaggcgct tgaaccggac gccctcgcgg aagaggcggg
     1921 cgttctcgat cgcgccggcc gcgcggacgg cgaggttctc ggcgaacgcg acgtcgtccg
     1981 gctcgaagcg gcgccccgaa tcgctcgcga cgagggtcag gacgccgagg gtccggccgg
     2041 ccgcgatcaa cgggaccgag atgtaggagc gcagggcgag ggcccggatc atctcgaggt
     2101 ggcgttcgtc gcgggcggcc gcatcgagga cctcgtccgg gatctcggcg acgtattcgg
     2161 acttgccggt gcggaggatc tcggcgacgc cggacgtcgc gtcgggatcg gccgggtact
     2221 cctcctggag gcggacggcg agggccacca ggtccggatc gcggtgggcg accgcgatcc
     2281 gacgggggag gccgtcctcg ctggcgagct ggacgacgca ccagtcggcg aagtccggga
     2341 cggccaggtc ggccacggcc tggacggtcg cctcgtagtc gagcgacgag gcgaggacct
     2401 cggacgcgcg ggcgaggaac gtctcccgct gctgggcctg gatccgatcg gtcacgtcgg
     2461 cgtaggtgac caccgccgtc tcgatggcgc ccgtaggacc cgggatcggg gcggcgctca
     2521 ggccgtaggt ccgccgcgac ccgccgtcgt aggcgatctc gatgacctcg ttctccacgg
     2581 tctcgccgcg catcgcccgg cgcagcggcc agtcctccgg gccgagctcc gagccatccg
     2641 gccggaagcc ccgggcggcc tcgtaggcgg gcgtctcgcc cagctcgacg ggcagcgggg
     2701 agaggcgctg ggccgcctcg ttggcgtaga ggaaccggcc gtccgggacc gccgcgagga
     2761 tcaccccgat cggcatccgg cggaggactg attcgagccg ggctcgttcc gcctcgttgg
     2821 cggacagcag cagctggcga accgagccga gtctaaggcg gacgatcgcg ccggcagcga
     2881 ggtcggcata ggcccgcacg agacgctggt ccgccggcgc gaacgtccgc ggctcgcgga
     2941 aaccgagcca cagcccgccg agcggcgcgt ggtcgccgac gagcggcacg gcgcagcccg
     3001 acgcttcgac gccgtccttc gaacgaccat cgacccagac cggacggacc ttccggacgg
     3061 cggtcgcgag cggaacgtct gcgtcgaggg acagggaacg ggcggccccg ccgggtggct
     3121 ggccgtcgtt ctcgtcgggt ggccggccgc tctcgcgaac gacgaggtcg agccgggtgg
     3181 cggtctcgtc gagcaggccg acgacgaccg actcggcgcc cagcgcctga cgtgcttcgt
     3241 cggccacgac cgcggcgaca tccgcgatcg tcgtggcatc ggccagcgtc cgggtcaccg
     3301 tctggagccg ctccatccgg tctgccagct cctcggccgc tcgtcgcgcc agctgttctg
     3361 cctcgtggga gcggccgttg gcgatcgcga gtgcgatccg attcgcgatt tcgagcgtcg
     3421 ccgcgacggc cggctgatcg aaggaccggt catcgaccgt cgcgtacacg atcgagccga
     3481 tgacgtcgcc acgggcgatc agcggctggg cgatcgcact cctgatgccg gttgcctcga
     3541 gcatctcgcg cagctccggg tccggtggtg tctcggtcgc ccaccacgcc tcgatgtcga
     3601 gagcgaccac ggcgctgccc gatgcgagcg cccgaccgct gccacgcacc ggactgccca
     3661 ccgacggctg gtcgcgaagc cgctccacaa gttcccggag acgttcgtcg cgggcggcga
     3721 gtgcggcgcg gcgaaccgtg ccgtcggcga ggacgaggtc gacgacgcac caatcggcga
     3781 cgacggggat cgccaccatg gcggcgacct ggatgatcgc ctcgaggtcc gcggcctcgt
     3841 cgaggcgcag cgccgcgtcc gccagcatcc gggcgatctc ctccgcccgt cgccgacctt
     3901 cggcgatctc ctgggtgctg cgttcgctgg cccggatccg tcgctcggtc tcgatccgcg
     3961 acgtgacgtc atggaacgtg ttgatcgcga cccgccggcc gtcgcccagg gtcgcggcgc
     4021 gcgcgaggag catcgaccag cgtatgtcgc cacctgccac cgggctgaat ccgatgatga
     4081 ccggatcgac cggttcgcct gccagcaccc gtcgacccgg gagaagcgaa cgatcgaagg
     4141 gccggccgtc ctcatgggtc agctccaggc gctcgaccag ctcgtcgggg gtcgccgcga
     4201 gcatctcggc cgaggagccg aagccactga gacgcgcggc gtcgtcgttc gcgaacagta
     4261 gccggccgcc ctcgtcctgg acggtgaccc cggccgcgat gccctggagg atggcccgca
     4321 ggtcgaccgg cagcgggccc ggcccgagcg gtggatgcgg tccgccgtcc acgcccccgg
     4381 acgcgacctc tgccgttacc tccatggttc gcgatgcacc ggatccctcg tgtgacgcgc
     4441 ctgacgcccg gccgcggcgt cccgttgcag tagcgcagcc gcgcggtcgc agggcgatgg
     4501 atttcgatac cggaagggtt gcaacggcgt tgcaatcgaa cgaggcggga tcagggtgac
     4561 gcgggcgtgc cccgtgcggc ccctcgggag ccggtgcccg cgtccggttc atcggccgca
     4621 ttcgattcgt cggcggtcgt ttcggtggcc gtcggaccct gggcgaccgg gatttcccgt
     4681 gcctggaggc cctcgatgag gacctcgccg accgccgcga tcggaacggc gagcagtgcg
     4741 ccgatgaagc cgcctgccgc gcccccgatc aggacgctca ggatcacgag gaacggcgag
     4801 atcccgaccg tgttccgcat gacgagcggg accaggaagt tgccctcgat gagctggagg
     4861 accaggtagg ccccgccgac ggcgagggcg agctgcgggg aaacggtcgc cgcgacgagc
     4921 acggccggga tcgccccgag aaggggaccg acgatcggga ttgcctcggc gatcgcggcg
     4981 atcaggccga gcagcagcgc cgccggcacc ccgagcaggg tgtaggtgac ggccgtcgcg
     5041 acaccgatcg tcgccatcag gatgagctcc ccgcgaaccc acatgccgag cctggtctcg
     5101 gccgcgttcc agacgtcccg ggcgcgcttc cggcgatccg cggggacgaa tgcgagggca
     5161 taccgctgga gcctggcgtg ctcggtcagc cagaagtaga cgacggtcag gagggtcagc
     5221 agggaggtga cggtctcggc cagcgtcagc ccgacctgga cgacctgacc cggcgcaggc
     5281 gccgcattcg gcggcggctg gagcgcccgg gaagccgagt cgatcagggt cgtcacgctc
     5341 cgcgacagac ccggcgggct gatcgtcgtc gcccattcgc gagcccgctg gaagaacggg
     5401 ggcagcgagg ccatcgtccg ctcgagctgg gcgatcgcca ccggcagcac gatcagggcc
     5461 atccccacga cgaccgcgag gaaggcgccg tagacgagca ggatcgtcgg ccctcgaccg
     5521 atcggcacgt ggccgcggat ccaggcgacg aagggctgga ggcccgatgc gaggatcacc
     5581 gccacgagca tcaggatcag gacatggccc gcggcgattc cgagcagcac gagcccggcc
     5641 acgaggccga ccccgaccgc caggccggcg ccccgggtga ccgaccggcc gaagccgtcg
     5701 tcgagcgtca cggggcgggt gtcgggtcag gaacgggggc agcgctcgcc cgccgcgcga
     5761 tcaggcggtg gacggtgctt cgcagggccg cgcgcacgcc gggctggatc ggtcgatcgg
     5821 gaagcggctc gtccaccagc ggaggatcga gcgaccggcg tcacggcccg tcgagcgggc
     5881 gtgacaacgg cattgcagat ccgtatcgga cccctaccga ggcggcgatt cggccgatgt
     5941 cagggcgccc tgcatgtgga atgcaagggc gcttgacgat ccgcgtcggg gttcgctaca
     6001 ttctggtccc acctccactg cgcatcccgg atcgccgcga tccagccggc gctccccacc
     6061 tggagacgac caaccgtgga tctcggcgca ctcgtcagcg gtctgctgac cggccttcgc
     6121 gagggcgtcg aggccgcgct gatcgtcagc atcgtgatca cctacctcgt ccgtagcggt
     6181 cgccccgagc agatcggccg ggtgtggatc gggacgggac tcgcggcggt catcagcctc
     6241 gtcgtcggaa tcgtgatcta cagcacggtc ggcgccttcg aggcgccgta cgaacagatc
     6301 ttcgagggga cgacgctcct ggtcgccgcc gttgtcgtga cctggatgct cttctggatg
     6361 cgccgtcagg cccgctccgt gaagagcgag ctccaggcgg ccgtggaccg gacgatcgcc
     6421 ggtggcgcgt cctggggcct ggcggccctc gcgttcaccg ccgtcatccg cgaagggctc
     6481 gagacctcga tcttcctcgt cggccaggcc gcctccaatc ggaccgaggc cggctggatc
     6541 ctcgtcggcg ccttcgccgg tctggccatc gcgatcctga tcggctacgg cttctaccgc
     6601 ggcagcagcc gcctgaacct cgcctcgttc ttccgctgga ccggcatcgg cctggtgttc
     6661 atcgccgccg ggctcctcag ccacggcatc ggcgagttca tcgagatcgg cgccctcggg
     6721 aacgggccgt ggaccgcgac cgcgttcgac atcagcgggg tcctctcgga cgagaccggc
     6781 gtcgggtcgt tcctgcgagc gatcttcggg tactcggccg cgccggccgt cctgaccctc
     6841 gccgcccacg tcgggtacct cgtggtgatc ctcgccctct tcctccggcc ggcgcgccgg
     6901 cccctgccgc cggccccgtc cgcacctgag acggtcacgg cgcggtcctg acctcgtgac
     6961 cgacgacagc gcgaccgtgc cgccggccgg cgggccgatg atcaccttcg cgatcgcctg
     7021 accgcccggc gacggagcgg tccggggtcc cgatccccgt cgcagtccgt cgctcagcga
     7081 gccgtcgcgg ccacccgcag gatcgcgccg ccgccgatgt ccgcgacgaa gagctcgccc
     7141 gcctcgtcct cgccgaacgc actcgggttg cggccggtct cgccggccag gaccggcgcc
     7201 cggtacccgc cgcccgccgg gtcgatcgcc cagacccggc cgctgcaata gtcgccgaaa
     7261 acgtagccgc cgagcagcgc ggtctggacc gatccgcggt agacgccgcc gccgatcacc
     7321 gtgcagccga gatcgtggcc gtaatcggtg accggcagag tcagcgcaac accctcgcag
     7381 ccctgctccg gctggaagca atgggtgccc tccatccggt tccagccgaa gttcgtgccg
     7441 ggagcggtcc ctgctcgctg gacgtcgacc tcctcccacg cgttctggcc gacgtctccg
     7501 atccagaggt cgcccgtcgc ccggtcgaag ctcatccgcc acggattgcg gaggccccac
     7561 agccagatct ccggccggtg acggtccgcg ccgtcgcgga aggggttgtc ggccgggatg
     7621 cgatacgggt ggtcggcatc gctctgggcg acgtcgatcc ggaggatctt gccgaggagc
     7681 gtctcgagcg actggccatt gccctggggg tcgccaccgc tgccgccgtc accgagcgag
     7741 atcaggagca tcccgccggg atcgaacacc agggccccac cgttgtggtt ggcgaacggc
     7801 tgcttgacgt ggaggatgcg catctcggag gtcggatcga cccggtccgg atcgccgccg
     7861 ctgacctcga acgacgaaac ctgggtgtcg ccgttcgtgt cggtgtagtc gacgaagatg
     7921 cgcccgtcgg cagggtagtc cggatggaat gccaggccga ggagaccgcg ctcgccgccg
     7981 ctcgtgatct cgtcgctgat gtcgaggaag ggcgtcgcct cgacccggcc gttccggatg
     8041 atccggatcc ggccgccctg ctcggccacg aacagccgac cgctgccgtc gccggcgttg
     8101 gcgatcgcga ggggcgcggt cagaccatcg gcgaacggct cgagcgtgat cgcgacgcgg
     8161 gtcggatcga acccggagcc gcctgaccgg ctcgagcccg gcggcaggcc cgtggggcca
     8221 ccggccgtgc cgcctccgcg tggcgcagag cggctggcaa ggagcgcgat caggaccgcg
     8281 gccgcgatca cgacgagtgc gacgaggacg aggcgcacgg cggggccgct cgcccgggcc
     8341 ggggccgggc cggtggaagg gagcatcgat ccatcctacg gtcggccgcg tccgccggtt
     8401 cccgacgctg ctacattcgg ccgatggatg ccttcgagct ggccgatctc ggcgcacgac
     8461 gcgaggcgag cggcgccctc tacctcgagt tcctcagcgt gcccgacctg agcgtcgggc
     8521 tgtacgtcct ggcggccggg cagccggacc tccagcagcc gcacaccgag gacgaggtct
     8581 actacgtggt ttcgggtcgt ggccggatca cggtcggcga cgaggtccgc gacgtccgtc
     8641 cggggtcgat cgtgttcgtc gggacggccg tctcgcaccg cttccacgac atcaccgagg
     8701 acctgaccct gctcgtggtg ttcggccccg ccaagggctc gcgcgcgtcc ggctgacgag
     8761 gtcgtccggc ctgcgtgcat cgaatgaacg tccgcgacac gtgagagcgt cggtcacgct
     8821 caacgatgcc gattccgtgc atccaatgca ccgatccggc gaatcgacgg gccggaccca
     8881 ccctgagcgc ctcgaaaccg cccgatcggc ggccttcagg gtccatcggt tcgtctgatg
     8941 cacggaccgg tcgggttcgt gcgttggatg caccacgtcg acgcggccac gcgcgctgac
     9001 ctgcgctacc gagcgacgcc gaacccggcc gcgtcgccga gcgccgcgag ctcgagcgcc
     9061 agctccgccc cgcgcccgac ctcgtcgtgg cggaagtaga cgtacgcgtc gacgccgtcg
     9121 gccaggaacg gctccaggcg gtcgagccag cgggccagct cgtcggccgg atagtccgtc
     9181 cggcgcaggc ggagatacag gaacggtccg ctgcgacgga tcgacggcgg ctcggggtcc
     9241 gtgtcgaggt ccgtcgtcac gatggccgcg cccgcatccc gcgccgcccc gaagacctcg
     9301 tcgacgtgcc agctcgggtc ctggaactcg agggccagcg gcaggtcggc cggccacgtc
     9361 gcgaggagcc cggccagccg ctcgtcgctc cgggtcacgc cgtcggggac ccggaagagg
     9421 atcgtcccga gccgctcgcc gaaacgacga aggggcgtcg tcaaccagtc cacgctcgcg
     9481 gcgggatcca cctggagcgc gcgaaacgac gatcctcgct gggccttgac cgcgaagcgg
     9541 aagtcgtccg gggtcgccgc gagccagccg tcgaccgccg ccggactcgg ctggcgatag
     9601 aacgtgttgt tcagctcgac cgtcgcgaga cgcgaggcgt agtgggcgag gagcgccggt
     9661 ccgcgggcac cgggcggata gaaccgcggc gaccaagcgg gataggcgaa cccactcgtc
     9721 ccggtccgga gccgtccgac ggccacgatc cggctcaggc ctcggcgggc gagcaaccct
     9781 cgtgggtcgg gtgattgccg ctcgcgagcg gatccgcgac gcgcccgagg ctcgcctcga
     9841 ggccggccag gtccgccgcg tcgaggcggc cgaggaagta gcggcggacc ccgtcgaggt
     9901 gtgtcgtcgc cgcggaccgc agccgctcca ggccgagcgg cgtgagcacc gcctcctggc
     9961 cgcgcgcgtc ggtcgtgcag gccgctcgct cgaccgagcc ggcggcctcg aggcgatcga
    10021 cgagccgggt gatcccgctg cgcgacagga tgacccgctc ggcgaggacg ttcatgcgga
    10081 cgcggcgccc cggggcgtga gcgatctgga gcagcgcgtc gtactcggcg agggacaggc
    10141 cgtgggcgac ctggagctct tcgtcgagac ggcgggtaat ccgggcgtga gcggtgagaa
    10201 agcttcgcca ggcgccgatg cccggctcgt cggcgacgac ggcggccgtc gccggcccgg
    10261 cgatccgggc cttggcggca ggctgggtgc gtggcgaaac ggatggcgtc ggcatgtccg
    10321 cgccatccta ctcccgcgcg atgatcgttg acaagtcaac gacctacgca tatggttgca
    10381 cagtcaacga accgacccaa gcgatcacgc ggcggcgggc acgaccgaca cgagtcccgg
    10441 aggacaccga accatgagca cgacgacctg gaagctcgac cccgcccaca ccgacatcac
    10501 gttctcggcc aggcacatga tgatcacgac cgtccgcggc accttcgccg atgtcgacgg
    10561 caccctcgcg atcgacgatg ccgacccgac ggccgccaac ggcgagatcc gcgtccaggc
    10621 ggcgagcatc tcgaccgggt tcggcgcccg cgacacgcat ctgcgctcgg ccgacttctt
    10681 cgacgtcgag acctggcccg agatcgtcgt ccgcttcgac ggtgtcgagc cggtcaaggg
    10741 tgacgactac cgggtgaccg ccgacctgac gatccgcgcc atcacccggc ccgtgacctt
    10801 cgaggtcgag catgtcggcg agacgacgaa cttccagggc acgcgccata tcgcgttcac
    10861 cgcgcaggca acgatcaacc gcgaggactg ggggctgacc tggaacatgt ccctcgagac
    10921 cggcggctgg ctcgtcggca aggacatcaa gctcgtcgtc gaggtcgtcg cggacgaggt
    10981 cgtgctggcg ccggccgagg gcgagcgcga gctggtccgg gccgcggcct gatcccgggc
    11041 gggtcgaccg acccgcgagc gactacgatc aacggcggat cccgcgggtc cgccgttctg
    11101 attcgctcat ccgccgccgt gcgcttcgtg agggccccgt tccatgacca cgttcgacgt
    11161 cgaggcgctg cggcgccggt tcccggccct ggcgatcgag gacggcggtc gacccgtcgc
    11221 cctcttcgac ggccccggcg gcacgcaggt cccggagtcg gtgatcgccg cgatcggcga
    11281 ctactaccgg acgtccaacg ccaaccacga cggcccgttc ctgacaagcc ggcgcagcga
    11341 cgaatcggtc cacgaggctc accaggcgat ggccgacatg ctcggttcgg cgtcggccga
    11401 cgagatcaag ttcggcgcca acatgacctc gctgacgttc cacgtcagcc gctcgatcgg
    11461 ggcgacgatg gcgcccggcg acgagatcgt cgtgacgatc ctcgaccacg agggcaatgt
    11521 cgggccgtgg aaggcgatcg ccgccgaccg cgggctcgtc gtccggaccg tggacatccg
    11581 cgtatcggac gtgaccctgg acctcgccac gctcgacgcc gtcctcgggc cgcggacgaa
    11641 gctggtggcg gtcgggtacg cctccaacgc ggtcgggacg atcaacccgg tcgccgagat
    11701 cgtccggcgc gcccacgcgg tcggcgcctg gacgtatgtc gacgccgtcc atgccgcacc
    11761 gcacctgccg atcgacgtcc gggcgatcgg gaccgacttc ctcgcctgct cgacgtacaa
    11821 gttcttcggc ccgcacgccg gtgtcctcta cggccgggcg gagatcctcg agtcgctgcc
    11881 gccgtacaag ctccgcccgg cgaccgaccg gttcgagacc gggacaggca acttcgaggg
    11941 actggccggg gtgacggccg cggtcgagta cctcgccgac atcggctcgg cctacggcgg
    12001 ggcgagcggg gtcgccgcgc gtcgcgaacg ggtggtcgcc gggatgaccg ccatccgggc
    12061 gtacgagctc gatctctacc gccgcctggc cggcggactc gaggccatcc ccgggctgac
    12121 gatgtacggc atcaccgacc gggcgcgctt cgacgagcga accccgaccg ccgcactgcg
    12181 acttgccggt gtcgcgccgc gcgacgtggc cgaggcgctc ggcgccgagg ggatcgcggt
    12241 ctgggacggc gatttctacg cgaccgggct gatcgagcgg ctgggcctgg cggaatcggg
    12301 cggtgtcgtc cgcatcgggc tgacccacta caacaccgcg gccgaggtcg accggctcgt
    12361 cgcggcgctc gggcgcatcg cggcggccgc aggagcacgg gcaacggcgg cagcttcggc
    12421 ctgaccgacc cgggccctga ccgacccgcg cccggtcgac cgcgcgatgc cggccgtgga
    12481 cgtcgtcgtc atcggcggag ggatcgtcgg gaccgcgatc gcggccttcc tcgccgaggc
    12541 cggcctccgc gtccggctcc acgagcagga gacgatcgcc gccggcgcct cgggccgcaa
    12601 ctccgggatc gtccagcacc cattcgatcc ggtcatggcc gggctgtacc gcgggacgct
    12661 cgcggaatac cggcggctgg cggatgattc cgccgcctcg ttccggctcg acgatgagcc
    12721 cgcgggactc ctctacgtcg gccgcgaccg cgccgcggcc gagcggacgg ccgccacctg
    12781 ggcggaggcc tggccggcca gcgcgcccga gatgctcgac ggcgtcgcat tgacccgcct
    12841 ggaacctgcg ctcgcgcccg atctcgtcgc ctgccggctg gcgatcgggt ttcccgtggc
    12901 tccggcggcc gcgaccgagg cattcgcgag ccaggcccga cggctgggcg tcgagatcat
    12961 cgtcggcggt gggccggctc ggccggcgat tggtggcgac cgggtgcgag ggatcgagtg
    13021 gggcggacgg atcgagccgg ccggggtggt cgtcgtcgcg gcgggtccgt ggacgcccga
    13081 tgtcatcgac ccgggcggct cgtggcgtcc gatccgttcg tcgtggggcg tcgttgccag
    13141 cgtcgtcgtc gcgaatgcgc cacgccacgg tttggaggcg atcgacatcg acatcgagcc
    13201 atcggagggt gacggccgag cccccgacca cgccgggccc gacgaggcga tcgagttcag
    13261 tctcgtcccg gctgcgggct cgagcgccct cggctcgacg ttcctgcccg acgagcccga
    13321 tccggacgcg tggctcgacg cgctccgccg cgtcggcagt cggtacgttc cgggcgtcgc
    13381 cgacgcgccg ctgctcgggc tgcggcgctg cgctcgtccg gtgagccgcg acggccggaa
//
LOCUS       DATAIW010000021        12087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_119500, whole genome shotgun sequence.
ACCESSION   DATAIW010000021 DATAIW010000000
VERSION     DATAIW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119500"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VHM48_00735"
     CDS             <1..382
                     /locus_tag="VHM48_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2753954.1"
                     /translation="WATVGHTAAGRTYWLCGVVGEPGLETTPWPDPDGDSWEDDLDHP
                     RTAAELVWALDSAWAIIDGCLDRWTPELLDDTVERHFGEQVQVHSRASILQRLLTHEA
                     YHCGELSQTLGIHGLPQIDLWRPD"
     gene            476..697
                     /locus_tag="VHM48_00740"
     CDS             476..697
                     /locus_tag="VHM48_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX2753955.1"
                     /translation="MERTTVYLTEAQKRGLERAARLTGRSEAELIREGVEVVTSRHQV
                     ADPVLPLFESGQPDLAERVDEALDGFGEP"
     gene            694..1113
                     /locus_tag="VHM48_00745"
     CDS             694..1113
                     /locus_tag="VHM48_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2753956.1"
                     /translation="MIVLDTSGLLAAIDASQRHHAAAAAALRASDGPLLLSPFVLAEL
                     DYLLSTRVSSRAATSLLGQVADGAYRLEPMAAEDIARATEIIGRYRDLELGLADASLV
                     VLAERHGIHDVLTLDERHFRAIDGPGGRPFRILPADA"
     gene            complement(1308..1733)
                     /locus_tag="VHM48_00750"
     CDS             complement(1308..1733)
                     /locus_tag="VHM48_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX2753957.1"
                     /translation="MSTASIVYRSRTGTTRQLAEEIASHLRTRGVEASVMSVGDCDIQ
                     SLAEVDFLLLGCWTGGLFVIAQHPDEPWLAFARDLPGLARPRVGLFTTYKLVTGSMFA
                     RMRAALEGKISRFDLELKSRNGHLTSAGRLALDQFVGVG"
     gene            complement(1738..2214)
                     /locus_tag="VHM48_00755"
     CDS             complement(1738..2214)
                     /locus_tag="VHM48_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2753958.1"
                     /translation="MTTTIQQIEDFLALKRVAVVGVSRNPKELSYTLWQELRQRRYEA
                     VPVNPATSEIDGQRCYASVAEIEPPVDGALIMTPPAVAERVVEECVGAGVTHVWLYGG
                     FGGAGATSKTAIEYAEAHGVDVVARLCPYMFLPGTPFFHGLHAAGKKLTGSYPARG"
     gene            complement(2636..2998)
                     /locus_tag="VHM48_00760"
     CDS             complement(2636..2998)
                     /locus_tag="VHM48_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753959.1"
                     /translation="MDEPAVRARAQAVCDALAAGDIDRATGDFSPELRRNLGEVLALM
                     PLPANEVEIDSIEHSGSGYNVVLRMAGEVDEVLIQTRWKDRDGEPRMVEASHLASTPI
                     ATDDDATDEPADAANEGA"
     gene            complement(3092..4081)
                     /locus_tag="VHM48_00765"
     CDS             complement(3092..4081)
                     /locus_tag="VHM48_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753960.1"
                     /translation="MAMHLPTPRAAPAVIPLTTARFGTNATDGGLQTGTDGLLLPIAL
                     AVVAVVAVVAGAFLLTMVRRHRQAGPAAEAAESDDAAESTLGSSAARDRNLPRWLDRS
                     VADARFRTDTTTAVRAAAALATSSARLPVVFTGPIDEGIERALVRYDSVPLLDRPDDV
                     LGQTLRELDGGDEVEVLERVEVWARVRTPNGGAGWLSTMTLAAVAAGTTDDDLDADDP
                     DEPGPPAPVDELPGLESILEAIAAQRLARSGPATSIRGETAVSVDAEPTAPKRTRSRK
                     PKSDQPTPQRVARQELASRSDPVPAADLDAAPAAPTRRRPRTPTSERRAAGPR"
     gene            complement(4232..4594)
                     /locus_tag="VHM48_00770"
     CDS             complement(4232..4594)
                     /locus_tag="VHM48_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753961.1"
                     /translation="MVGADAAFDFLADPVRLPEYVPTMRLEDSTAVEGELDVDADLRE
                     RGGAPDAGFVADRPTRRIEWGRPGSDYGGSIVVAAGTTNTADVTIRLRTRDDANVEEV
                     SRVFDQAISNIRRLLLRR"
     gene            4755..5348
                     /locus_tag="VHM48_00775"
     CDS             4755..5348
                     /locus_tag="VHM48_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX2753962.1"
                     /translation="MTTRRVAWRRSDEVEADEHCTLTTRDGGLSLVGTVIGAEGGLPV
                     RVEYRVLADRAGLTTAAHVRDLRGFTTRTIVLERDAKDRWSVDGTVQRSLKGCTDVDL
                     GCSPSTNTLPVRRLRLAIGASHTIQAAFVRFPELAVVKAAQTYTRLDEFTWRYASGTF
                     EADLTVDDDGLVAGYAVWRRTGYAIGPDDTEPLDTHR"
     gene            complement(5389..6450)
                     /gene="add"
                     /locus_tag="VHM48_00780"
     CDS             complement(5389..6450)
                     /gene="add"
                     /locus_tag="VHM48_00780"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2753963.1"
                     /translation="MTSPNPASGDRFDLIPKVELHCHVEGTVRPATVVELAAKAGRPL
                     PVEDPADLYRYDSLDAFLAVFWLVQETLLTRDDWARIAYESLIDGAAHGLRYRETFFT
                     PARHLAGGQDLATIVAGLTAGIEAAEAAAGVRCLLIADMDRAYGPAAGLELVDQLGEL
                     RRSGRAERVIGVGADSTELGIDLASFAPAFGAARRLGFRRTCHAGEAVGVGPDNIRIA
                     LDVLGAERIDHGVAIVEDPSLMERVAAERIPLTVCPTSNVVIANRFRTLAEHPFRAMR
                     AAGLLVTINTDDPAMMDLDLGLEYARVADAYGFDLAELGRIAVEGIESTWLDESDRRS
                     LVHEFEAALTIDPAPSERR"
     gene            6631..8442
                     /locus_tag="VHM48_00785"
     CDS             6631..8442
                     /locus_tag="VHM48_00785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX2753964.1"
                     /translation="MDRARAAAPIKRRPSVLRRGPGPILAVLVVLLAVPRPALAGPPA
                     ISLVTVGSPVGTTPQNHQNEPAVAMDAHQPNVLAAGVNDFIDWQPCPEDTARESGTCR
                     GSNAGVGLSGVYFSFDSGHSWIQPTYTGLTSRDCTAAGPCTPHTGPIGTLPWYAEAGL
                     ASNGDPAVAFGPKRVGGTFSWANGSRLYYVNLMGAVTATFPQKEPFKGFLSLGVSRLD
                     NPTPTSVLDKNSWMAPVIVANKTSATTFEDKEQVWADNAETSPFFGNVYACVDEYRSN
                     GRGNAFSQTEQVIRSSDGGDTWTMKQVRSATANVAKGFRGACNIRTDSHGVVYLFYTH
                     FEVGSSGLGAHTMQKSLDGGKTWTQPRDVQPANDGCYNIDVVSGRCVADGGAGARIDL
                     LSMPSIDIANGAPTGVDATDEIVDAWGDGRFGLNHEVTLLSYSTNGGANWSPPATISL
                     PGDRPIYSAPAIAPDGSAVYVMYEALTDPWRGADMSSARGYHGIFLRAPIGAGGAPGA
                     WTTLVTGPLGDIRASYPGHDIYQERVGDYVYAAASRTYGVGVWSDTSNAAVCPAVQAW
                     RAASYAANERVLPGAPWPTVDCPPTFGNVQIMAATSG"
     gene            8667..9188
                     /locus_tag="VHM48_00790"
     CDS             8667..9188
                     /locus_tag="VHM48_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2753965.1"
                     /translation="MTIRPARPADFAAMQEIEVEAGSMFAAIGMDSIAEDEPFTLDEL
                     AAYTAADHAWVAVDDDDRPVGYLVAELVDGRAHIEQVSVRPSRARRGIGRRLVDHVAT
                     WAAAEGLPALTLTTFADVPWNAQLYERLGFRRLDEDELSSGLRHLRDEEAARSLDRWP
                     RVAMIRVVAPPAG"
     gene            9404..10366
                     /locus_tag="VHM48_00795"
     CDS             9404..10366
                     /locus_tag="VHM48_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753966.1"
                     /translation="MRVNRRFLYWGVFLVAIGGVLVAADLTTDDRGFILNALRLWPLA
                     VVAIGLGLVLRRTRFSLPGGLLAAAVPGLVLGGAFAVAPHLAVDCGATGSPSSSATRQ
                     GSFDGPARISVSTGCGSLVVDTASGSAWRLDAGNAGSRSPIVDASAHSLSIDADGAEG
                     WHVFDRTRDVWRLTVPTTAIDDLSLAVNAGTGRISLTGARIGTLDLTTNAAQASVDLT
                     GATLATLTGVVNAGMTSIRLSETADVVGSMEVNAGSLQVCAPTGLGLSVHHSGALSGM
                     QINGLHEAGANWHSPDYASAAHRADLNVTVNLGSVEINPIGGCK"
     gene            10357..10860
                     /locus_tag="VHM48_00800"
     CDS             10357..10860
                     /locus_tag="VHM48_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2753967.1"
                     /translation="MQVNRRLYRCRHNRQIAGVASGLAEYFDLDPTIVRVLWILSFFL
                     GGVGLLLYIAMAIIVPLEPETGVATAGPAAGPRAGDSTGGDPAASPTDWHSMPATHQH
                     ASRGAGGGRAATFVGIALILFGALALVDTVLPAWADGGRFLWPAFIIGIGALLLATAV
                     RREPTES"
     gene            10857..>12087
                     /locus_tag="VHM48_00805"
     CDS             10857..>12087
                     /locus_tag="VHM48_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX2753968.1"
                     /translation="MIGGAALVVGLLLILAGLASARIPGGAAFAPSGIDHALEIEDDT
                     GSGPLRLARLARNPIRAFLLSPIHAATWHANAAVGQGFFVGLFAFATLVSIASAGFAT
                     LVAGIGFVFIALAIEGSRLVARIERRRFRFGDPGGLRAHPYRPLRGGILDILRAEFGD
                     ENRWRDVLYVGVNFPLSIIEFVVIVTVWSVSLWLLTMPLWYDAGAGSTLPAFLGALAG
                     HDAPTVVIRTLAGAALLPVAASLSQLVMALHRAVVDGLLCTSESRELRREVESLKKSR
                     SAVLDVEASELHRIERDLHDGAQQHLVMLTIDLGLASERIDTDPEGAKELVLAGQEQA
                     RQALAELRNLVRGIAPSILLDRGLVAALGSIAARGPVPAVVRSGLEPGERLPDGIERA
                     AYFVATEALANVAKHSGA"
BASE COUNT         1774 a         4286 c         4163 g         1864 t
ORIGIN      
        1 ctgggcgacg gtcggccaca cggcggccgg ccggacgtat tggctctgcg gcgtcgtcgg
       61 cgagccgggc cttgagacga cgccgtggcc tgatccggac ggcgatagct gggaggacga
      121 cctcgaccac ccgcggaccg ctgccgagct cgtctgggcc ctcgactcgg cgtgggcgat
      181 catcgacggc tgtctcgatc ggtggacgcc cgagctgctc gacgacacgg tcgagcggca
      241 cttcggcgag caggtccagg tccactcgcg ggcgtcgatc ctccagcgcc tgctgacgca
      301 cgaggcgtac cactgcggcg agctctcgca gaccctcggc atccacggcc tgccccagat
      361 cgatctctgg cggccggact gatcgccgtg ccggggtaac gcgtgcgtta ccccgcgccg
      421 tgccacaggc gatcactcac cggcacgccg ggcgatatat ggtatcgtgt atggcatgga
      481 gcgaacgacc gtctacctga ccgaggcgca gaagcgaggg ctcgagcggg ccgcgcggct
      541 gaccgggcgg agcgaggcgg agctgatccg ggaaggcgtc gaggtcgtga cgagccgcca
      601 tcaggtcgcc gatccggtcc tgccgttgtt cgagagcggg cagcccgacc tcgccgagcg
      661 cgtcgacgaa gcgctcgatg ggttcggcga gccgtgatcg tcctcgacac gagcgggctc
      721 ctcgcggcga tcgacgccag ccagcggcac cacgccgcgg ccgcggccgc gctccgcgca
      781 tcggacggtc ccctgctgtt gtcgccgttc gtgctcgccg agctcgacta cctcttgtcg
      841 acgcgtgtct cgtctcgggc ggcgacgtcg ctcctgggcc aggtggccga cggcgcctac
      901 cggctcgagc cgatggcggc cgaggacatc gcccgggcaa cggagatcat cggtcggtac
      961 cgagacctcg agctcggcct cgccgatgcg tcgctcgtcg tcctcgccga gcggcacggc
     1021 atccacgacg tgctgacgct cgacgaacgg cacttccggg cgatcgatgg ccccggcggt
     1081 cggccgttcc ggatcctgcc cgccgacgcc tgacccgccg cccggcggcg caccgcaggt
     1141 ctgcaccggc ggcccggcct acaactcctt caacgggcac ggccaggtga acgccctgtc
     1201 agccatcctc aggtagtcaa ccgcgcagtc ggacgatgcc ggcggcccat ggtctccggg
     1261 gtcgtcgcgt cccggttcgg ccatcaagcc acccgagccg gcttccccta ccccaccccg
     1321 acgaactggt cgagcgccag tcgcccggcg ctggtcaggt gcccgttccg cgacttcaac
     1381 tcgaggtcga accgcgagat ctttccctcg agcgccgcgc gcatccgggc gaacatcgag
     1441 ccggtcacca acttgtacgt cgtgaacagc ccgacgcgcg gccgggccag gcccggcagg
     1501 tctcgcgcga aggccagcca cggctcatcg gggtgctggg cgatgacgaa gagaccgccc
     1561 gtccagcacc cgaggagcag gaagtcgacc tcggccagcg actggatgtc gcagtcgccg
     1621 accgacatga cgctcgcctc gaccccacgg gtccggaggt ggctcgcgat ctcctcggcg
     1681 agctgccggg tggtgcccgt ccggctccga tagacgatgg acgctgtgct catcgcccta
     1741 ccctcgcgcc ggatagctgc cggtgagctt cttccccgcc gcgtggagtc cgtggaagaa
     1801 cggcgttcct ggcaggaaca tgtatggaca gagccgggcg acgacatcga ccccgtgggc
     1861 ctccgcatac tcgatcgccg tcttgctcgt ggcgcccgct ccgccgaacc cgccgtacag
     1921 ccagacgtgg gtcacaccgg ccccgacgca ctcctccacg acgcgctccg cgacggcggg
     1981 cggcgtcatg atcagcgctc cgtccacggg cggttcgatc tcggcaacgc tcgcgtagca
     2041 gcgctggccg tcgatctcgg aggttgccgg gttgaccggc acagcctcgt agcgccgctg
     2101 ccgcagctcc tgccagaggg tgtagctcaa ttccttcgga ttgcgtgaca cgccgaccac
     2161 ggccacccgc ttgagtgcca ggaagtcctc gatctgctga atggttgtcg tcatcgctcg
     2221 cctcctcgtg tgggatcgcg cacgaagact tagcaagggg cggtgccgga cgacaagtgc
     2281 tggacggctc cacctcgttc gccgcctctc ccgcctcgac ttgggccatc aatgctccct
     2341 tgacgatggc gacacgcgat cggctggtgc tcgagaagtc ggggaacgcg gtaccgggga
     2401 tccaagcctg tggtacatgc gagcggctcg aaccgacgct gctcacccca cgcgacgacc
     2461 ggcaggtcgt gcgcggtgag tacgcccgtc ctccgaagcg atcacgccaa atgcgctata
     2521 ccgttgcagg tgcaacaact cgccattccc gaggacgttc ccgtcccgag atgttcgccc
     2581 tgagggacgc gccgttcgcg acgtgagtgc ggcgaagtat ggctccggtt ggccctcagg
     2641 cgccctcgtt cgctgcgtcc gcgggttcgt cggtcgcgtc gtcgtcggtc gcgatcgggg
     2701 tcgacgcgag gtggctggcc tcgaccatcc gtggctcccc gtcgcggtcc ttccagcgcg
     2761 tctggatgag gacctcgtcg acctcgccgg ccatgcgcag gacgacgttg tagccggacc
     2821 cgctgtgctc gatggaatcg atctcgacct cattggcggg aagcggcatc agtgccagca
     2881 cctcgccgag gttgcgccgg agctccgggc tgaagtctcc ggttgcgcgg tcgatgtcac
     2941 cagcggccag ggcatcgcag acggcctgag cacgggctcg aacggccggt tcgtccatcg
     3001 tgtcgacctc gtgatcggct cgcggccggg gtgggccgcc cggaacgatg ataggcccgt
     3061 tcgacccgag caaccgccat cgctcgcgaa gtcagcgcgg gccggcagct cggcgttcgc
     3121 ttgtgggggt tcgcggtcgg cggcgcgttg gagcggcggg agcagcgtcg agatcggcgg
     3181 ccggcacggg atcgctgcga ctggccagct cctgtcgcgc caccctctgc ggggtcggtt
     3241 gatcgctctt cggtttgcgc gatcgggtcc gtttcggagc cgtcggctcg gcgtcgacgc
     3301 tgacggccgt ctccccgcgg atgctcgttg cgggtcctga acgcgccagc cgctgcgcgg
     3361 cgattgcctc gaggatcgac tcgagcccgg ggagctcgtc cacgggggcc ggcggccctg
     3421 gctcgtcagg gtcgtcggcg tccaggtcat cgtcggtggt cccggctgca accgcggcga
     3481 gcgtcatcgt ggacagccag ccggcgccac cgttcggcgt tcggacgcgc gcccagacct
     3541 cgacacgctc gagcacctcg acctcgtctc cgccatccag ttcccggagt gtctggccga
     3601 ggacgtcgtc cggccgatcg agaagcggaa cgctgtcata gcgaaccagc gctcgttcga
     3661 tgccttcgtc gatcggtccg gtgaagacga cgggcaggcg agccgacgag gtcgccaaag
     3721 ccgccgccgc ccgaacggcg gtcgtggtgt cggtccggaa ccgcgcgtca gccaccgagc
     3781 gatcgagcca gcgcgggagg ttccgatctc gcgccgctga cgagcccagc gtgctttcag
     3841 cggcatcgtc cgactcggcg gcctccgctg ccggcccggc ctgccggtgc cggcgcacca
     3901 tggtcaggag gaaggctccg gcgaccacgg cgaccacggc gaccacggcg agcgcgatcg
     3961 ggaggagcag cccatcggtc ccggtctgca gcccgccatc ggttgcgttc gtgccgaaac
     4021 gagccgtcgt cagggggatg acggccgggg ccgcacgcgg agtcgggagg tgcatggcca
     4081 tgggttgcag gtgcaacagg atggccggtc gacggcggcc gtgtcattga cccatccgtc
     4141 acccatcaac ccagccaagg gtccgacaga ccgattcggt cagggccctg ccaggctgcg
     4201 tgagatggcc tgaatccgtc cagacgcgcg gtcagcgtcg caagagcaac cggcggatgt
     4261 tggagatcgc ctggtcgaag acgcgggaga cctcctcgac gttcgcgtcg tcccgcgtcc
     4321 ggagtcggat cgtcacatcg gcggtgttgg tggtccccgc ggcgacgacg atcgacccgc
     4381 cgtagtcgct gcccggccgg ccccactcga tgcgccgcgt cggccggtcg gcgacgaagc
     4441 ccgcgtcggg tgcgccgcca cgttcgcgga ggtccgcgtc gacgtcgagc tcgccctcga
     4501 ccgcggtcga atcctcgagc cgcatcgtcg ggacgtactc cggcagccgg acgggatccg
     4561 ccaggaaatc gaacgccgcg tcggcaccga ccatgaccgt gaaggttcgc tcgagatcag
     4621 ccagcttcgt gctcctttcc gcccatcagc atggcgcggc cgctatcgta cgggtggtga
     4681 ccgccgacgg cgcgagactc gaaccccacc cggacccgac ctcgaccgat cgaacaccga
     4741 ggcttgtcaa tggcatgacg acgagacgcg tggcgtggcg gcgatccgac gaggtcgagg
     4801 ccgacgagca ttgcaccctg acgacccgcg acggcggcct gtccctcgtg ggcacggtga
     4861 tcggcgccga gggtgggctg cccgtccggg tcgagtaccg cgtcctcgcc gatcgcgccg
     4921 gcctgacgac cgcggcccac gtccgggacc tgcgcgggtt cacgacgcgg accatcgtcc
     4981 tggagcgtga cgcaaaggac cgctggtcgg tcgacgggac cgtccagcgc tcgctcaagg
     5041 gatgcaccga tgtcgatctg ggctgcagcc catcgaccaa cacgttgccg gtccgccggc
     5101 tgcggctcgc catcggcgcg tcgcacacga tccaggcggc gtttgtccgg ttcccggagc
     5161 tcgcggtcgt caaggcggcc cagacctaca cccggctcga cgaattcacc tggcgatatg
     5221 cgagcgggac gttcgaggcc gacctgacgg tcgacgatga cgggctggtc gccggatatg
     5281 ccgtgtggcg gcggaccggg tacgcgatcg gtcccgacga caccgagccg ctcgataccc
     5341 accggtagag aaacgaaggg ccgtcgtcga ctgaccgacc tcgcgagctc atcgccgttc
     5401 cgacggcgcg ggatcaatcg tcagggccgc ttcgaactcg tgcaccagcg agcggcggtc
     5461 ggactcgtcg agccaggtgg actcgatgcc ttcgaccgcg atcctgccga gctccgccag
     5521 atcgaacccg tacgcgtcgg ccacgcgggc gtactcgagg ccgagatcga gatccatcat
     5581 cgccgggtcg tccgtgttga tcgtcacgag cagaccggcg gcgcgcatcg cccggaacgg
     5641 atgctcggcg agggtccgga accgattggc gatcacgacg ttgctcgtcg ggcacaccgt
     5701 cagcgggatt cgctcggccg ccacccgctc catcagcgac gggtcttcga cgatcgcgac
     5761 gccgtgatcg atccgctctg ctccgaggac gtcgagcgcg atccggatgt tgtcgggacc
     5821 gacgccgacc gcctcgcccg cgtggcaggt cctgcggaag ccgagccggc gggcggcgcc
     5881 gaacgccggc gcaaagctcg cgagatcgat gccgagctcc gtcgagtccg caccgacacc
     5941 gatgacccgc tccgcccggc cggaccggcg caactcaccc agttggtcca cgagctccag
     6001 gccggccgct gggccgtacg cccgatccat gtccgcgatc agcaggcagc ggactcccgc
     6061 ggccgcctcg gcggcctcga tccccgcggt caagccggcg acgatcgtgg ccaggtcctg
     6121 gccgccggcc aggtgccggg ccggcgtgaa gaaggtctcc cggtagcgaa gcccgtgggc
     6181 ggcgccgtcg atcaatgact cgtaggcgat ccgtgcccag tcatcgcgcg tgagcagggt
     6241 ctcctggaca agccagaaca ctgcgaggaa cgcgtcgagc gagtcgtagc ggtagaggtc
     6301 ggctggatcc tcgaccggca gcggccgtcc ggccttggcc gccaactcca cgacggtcgc
     6361 aggtcggacc gttccctcga cgtgacagtg cagctcgacc ttcgggatca ggtcgaatcg
     6421 atcgccggac gctgggtttg gagatgtcat ctggtcgcct cccgcctgcc tgcgtcggtt
     6481 ccgttcaacg tcgccggcgc gggcagcgcg tcaggcgatc atgcgccgcc agccattcgc
     6541 gaggcaaggc ggacgccatg gttgaccctc catctccagg ggcgtataac ccatcgaccg
     6601 gggacagcgg aggagtggag gatccgaccg atggaccgag cgcgcgcagc cgcccccatc
     6661 aagcgacgac cctcagtcct ccgtcgcggg ccaggaccga tcctcgcggt tcttgtcgtc
     6721 ctgctggccg tcccgcggcc ggcactcgcc ggtccgcccg ccatctccct cgtgaccgtc
     6781 ggaagcccgg tcggaacgac gccccagaac caccagaatg aacccgccgt cgcgatggat
     6841 gcccaccagc cgaatgtcct tgcggcaggt gtcaatgact tcatcgactg gcaaccctgc
     6901 ccggaggaca cggcgaggga gagcggcacg tgtcgagggt cgaatgccgg agtcgggctg
     6961 tccggtgtct atttctcctt cgacagtggc cactcctgga tccagcccac ctacaccggg
     7021 ctgaccagcc gcgactgcac ggcggccggt ccctgcacgc cgcacacggg accgatcgga
     7081 acccttccgt ggtacgccga agctggcctt gcctcgaacg gggatccggc cgtggctttc
     7141 gggccgaagc gagtgggcgg gacgttctcg tgggcgaacg gctcacggct ctactacgtc
     7201 aacctcatgg gcgcggtgac tgcgacgttc ccccagaagg agccattcaa gggcttcctc
     7261 tcactcggag tctcacgcct cgacaacccg accccgacca gcgtgctcga caagaacagc
     7321 tggatggcgc cggtgatcgt ggccaacaag acgagcgcca cgacgttcga ggacaaggaa
     7381 caggtctggg ctgacaacgc cgaaacgagc ccgttcttcg gcaacgtcta cgcctgtgtc
     7441 gatgaatacc gaagcaatgg tcgggggaat gcgttttcgc agaccgagca ggtgatccgg
     7501 tccagcgacg gcggcgacac gtggacgatg aagcaggtcc gctcggcgac cgccaacgtc
     7561 gcgaagggct tccgcggcgc ctgcaacatc aggaccgaca gtcacggcgt cgtctacctc
     7621 ttctacaccc acttcgaggt cgggtcgtcg ggactcggcg cccacacgat gcagaagtcg
     7681 ctcgacggcg ggaagacatg gacccagccc cgagacgtcc agcccgccaa cgacggctgc
     7741 tacaacatcg acgtcgtgtc cggccggtgc gtcgcggacg gtggcgccgg tgccaggatc
     7801 gacctgctgt cgatgccgtc gatcgacatt gccaacggtg cgccgaccgg cgtcgatgcg
     7861 accgacgaga tcgtggatgc ctggggcgac ggtcggttcg gtctcaacca cgaggtgacc
     7921 ctgctctcgt attcgacgaa cggcggcgcg aactggagtc cgcccgcgac gatctcgctg
     7981 ccgggtgacc ggccgatcta ctcggcgccg gcgatcgcac ccgacggcag cgccgtgtac
     8041 gtgatgtacg aggcgttgac cgacccatgg cggggcgcgg acatgtcctc ggcgcgcgga
     8101 taccacggga tcttcctccg ggcgccgatc ggcgccgggg gcgcgccggg agcctggacg
     8161 acgctcgtga ccggtccgct cggcgacatt cgcgcgagct acccgggcca cgacatctat
     8221 caggaacgcg tcggcgacta cgtctatgcc gcggcgagcc ggacgtatgg agtcggggtc
     8281 tggtcagaca cgtcgaacgc ggcggtctgt ccggccgtgc aggcgtggcg agccgcttcg
     8341 tacgccgcca acgagcgggt gctcccagga gcgccctggc ccacggtgga ctgcccaccg
     8401 acgttcggga acgtgcagat catggccgca acgagcggtt agtgaagttc agttagtcgc
     8461 ccgacacgcc ctggctgagt cacggctcgg cggtcgcgcc ccgtcccggg gccggaagcc
     8521 acagcccgac cgcacccggc agcgcgacga acagggcgac cgcggcgctg gccggggtca
     8581 cgaccgcgcc gacgagcagc agccgtcacg ttggcgcggc ctgcgacgat ccaccccgtt
     8641 ggaggacggc ccctggaatg cactcgatga cgatccgacc ggcccggcct gccgacttcg
     8701 cggcgatgca ggagatcgag gtcgaggccg gctcgatgtt cgcggccatc ggcatggact
     8761 cgatcgccga ggacgagccg ttcacgctcg acgagctcgc cgcgtacacc gcggccgatc
     8821 acgcctgggt ggccgtcgac gacgacgatc gcccggtcgg gtatctcgtc gctgagctgg
     8881 tggacggccg cgcgcacatc gagcaggtat cggtccgacc cagccgcgct cgccgcggga
     8941 tcgggcgaag actcgtcgac cacgtcgcga catgggcagc cgctgagggg ctgcccgccc
     9001 tcaccctgac gacattcgcc gacgtcccgt ggaacgccca gctctacgag cgtctcggct
     9061 tccggcgttt ggacgaggac gagctctcgt cgggactgcg ccatcttcgc gacgaggaag
     9121 ccgcccgcag cctggatcgg tggccgcgag tcgcgatgat cagggtcgtt gctcctcctg
     9181 ccggctgagg gccgccgctc gcttccgggg tgtggatagc cccaccctga agatgccggg
     9241 tggccggctg gtggcccccg tccggcgccc gtagcgtgag cacatcaggc acgagcagga
     9301 ccgatcggat cgtcgagcgg ccggcgtgcc cggagtacgc tcgatcgagc gccaacgccg
     9361 tgacggatcg cgtccgatcg tcgcggcagg aaggacatcg ctgatgcggg tcaaccgccg
     9421 atttctctac tggggggtct tcctcgtcgc cattggcggc gttctcgtgg cggccgacct
     9481 gacgaccgac gaccggggct tcattctcaa cgccctccgg ctgtggccgc tcgccgtggt
     9541 cgcgatcggg ctgggcctcg tcctgcgccg gacgcgattc agcctccccg gcggattgct
     9601 cgccgcggcg gtcccgggcc tcgtcctcgg cggcgcattc gcggtcgctc cgcacctcgc
     9661 ggtcgactgt ggcgcgaccg gctcgccgtc gagcagcgcg acgcgccagg gatcgttcga
     9721 tgggccggcg cggatctccg tctcgaccgg ttgcggctcg ctcgtcgtcg acaccgcgtc
     9781 gggcagcgcc tggcgcctcg acgcgggcaa tgcgggcagc cggtcgccga tcgtcgacgc
     9841 gtcggctcac tcgctttcga tcgacgcaga cggcgccgag ggctggcacg tgttcgaccg
     9901 gactcgcgac gtctggcgcc tcaccgtgcc gacgacggcg atcgacgacc tgtcccttgc
     9961 cgtcaatgcc ggcaccggcc ggatcagcct gaccggcgcc cggatcggga cactggatct
    10021 gacaaccaac gctgcccagg cctcggtcga cctgaccggc gcgaccctcg cgaccctgac
    10081 cggcgtggtg aacgccggga tgacctcgat ccggctctcg gagaccgccg acgtcgtcgg
    10141 ctcgatggag gtgaacgctg gttcactcca ggtgtgcgct ccgaccggac tcggtctgag
    10201 cgtccatcac agcggggcgc tcagtgggat gcagatcaac ggactgcatg aagccggcgc
    10261 caattggcat agccccgact acgcatcggc ggcccaccgc gccgacctga acgtcaccgt
    10321 gaacctcggc agtgtcgaga tcaatccgat cggaggatgc aagtgaaccg ccgtctctat
    10381 cgctgccgcc acaaccggca gatcgcgggc gtggcctcgg gcctcgccga gtacttcgac
    10441 ctcgacccga ccatcgtccg ggtcctgtgg atcctctcgt tcttcctcgg cggcgtcggc
    10501 ctgctgctgt acatcgcgat ggcgatcatc gtgccgctcg agcccgagac aggagtcgcg
    10561 acggcaggcc cggcggccgg cccccgggct ggtgattcga cgggcgggga tccggccgcg
    10621 tcccccactg actggcactc gatgccggca acccaccagc acgcgagccg cggcgctggc
    10681 ggtggccgcg cggcaacgtt cgtcgggatc gccctgatcc tgttcggcgc cctcgccctc
    10741 gtcgacaccg tcctgccggc ctgggccgac ggcggacgct tcctctggcc ggccttcatc
    10801 atcgggatcg gggccctcct cctggcgacc gcggtgcggc gcgagccgac cgaatcgtga
    10861 tcggcggcgc agccctcgtc gtggggctgc tcctcatcct cgccgggctg gcgtcagccc
    10921 ggatccccgg cggagcggcg ttcgctccgt cggggatcga tcatgccctc gagatcgagg
    10981 acgacaccgg gagcggaccc ctccggcttg cccggctcgc tcgcaacccg atccgggcct
    11041 tcctgctctc gccgatccac gccgccacct ggcacgccaa tgcggcggtc ggccaggggt
    11101 tcttcgtcgg gctcttcgcg ttcgctaccc tcgtgtcgat cgcgtcggcc gggttcgcga
    11161 cgctcgtcgc gggcatcggg ttcgtgttca tcgcgctcgc gatcgagggg tcccggctcg
    11221 tcgcccggat cgagcgccgg cggttcagat tcggtgaccc gggcggcctc cgggcgcatc
    11281 cgtatcgacc gctccggggc ggcatcctcg acatcctccg ggccgaattc ggcgacgaga
    11341 accgctggcg cgacgtgctc tacgtcgggg tcaactttcc tctctcgatc atcgagttcg
    11401 tcgtcatcgt gaccgtctgg tcggtctcgc tctggttgtt gacgatgccg ctctggtacg
    11461 acgccggggc gggctcgacg ctgcctgcgt tcctgggtgc gttggcgggg catgacgccc
    11521 cgacggtcgt catccgcacg ctcgcgggag ccgccctgct gccggtcgcc gcctccctgt
    11581 cccagctcgt catggccctc caccgcgccg tcgtcgacgg attgctgtgc acatcggaga
    11641 gccgcgagct gcggcgcgag gtcgagtcgc tcaagaagag ccgctcggcg gtcctcgacg
    11701 tggaggcgag cgagctgcac cggatcgagc gcgacctcca tgacggcgcc cagcagcacc
    11761 tcgtcatgct gacgattgac ctcggcctcg cgagcgagcg gatcgacacc gatccggagg
    11821 gggcaaagga actggtcctc gcgggccagg agcaggcgcg gcaggcgctg gcagagttgc
    11881 ggaacctcgt tcgcgggatc gctccgtcga tcctgctcga tcgcggtctg gtggcggcgc
    11941 tcggctcgat cgcggctcgg ggcccggtgc cggcagtggt ccggagcggg ctcgagcccg
    12001 gcgaacggct gccggacggg atcgagcggg cggcctattt cgtcgccacc gaagcactcg
    12061 ccaacgtcgc caagcacagc ggagcgc
//
LOCUS       DATAIW010000022         9650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_683, whole genome shotgun sequence.
ACCESSION   DATAIW010000022 DATAIW010000000
VERSION     DATAIW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(706..1896)
                     /locus_tag="VHM48_00810"
     CDS             complement(706..1896)
                     /locus_tag="VHM48_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2753969.1"
                     /translation="MSLFKMLTRILSIVGKELVSIVRRPGALISLVLGPFLIMAIFGL
                     GYSGFHRPLETIVVLPADSGLPTDAKSYQDVAGVGLRIALVTPDEAAAMDQLNRQLVD
                     VVVVAPSDVRANLEAGLRSQIDVRVNLTDPVQSNYAVFVALGLEAAVNRQVIEDAVDE
                     GKGYAIDHGVNDVYKIPANVIAAPTEAWIDNVAQSEPTVVQYFAPAVLALILQHLAVT
                     LIALSVVREKNSGLFELFRISPITTAELVTGKLIAFGLFAGAVAFATIALLVVGFNVP
                     FLAQPGWIALVAGLLVAASLGLGLVIAAVSDSEAQAVQLTLLVLLASVFFSGFVLAIS
                     EFSEPVRSLIYILPVAGAIRLFGDFMFRGGTVVIWQIWVLAGLSVAYIGGAWLLLRRV
                     VARA"
     gene            complement(1893..2972)
                     /locus_tag="VHM48_00815"
     CDS             complement(1893..2972)
                     /locus_tag="VHM48_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2753970.1"
                     /translation="MASDPPPIEPTNERPPDGGLEADRDVIVAMHQVSRRFDDREVLS
                     GVDLKVRRGTILGIIGPSGAGKTTTIRMLTGAIAPTSGEVLVMGRHPKAFRRADRERI
                     GYMPQSFTLYEDLTAGENIDFVASLFGMLWRRRRRRVREVLKLVDLWDARGRQAGRLS
                     GGMQRRLELACALVHEPDLIILDEPTAGVDPLLRGSIWTELHRLRDVGRTLLVTTQHV
                     SEAEQCDAVAMIVNGRIIALAPPDELRRLATDGDLLDIETSALFDASVLVNTAGVRQV
                     NQDGPRHVRVVVDDAGSFLPVVVETIRAAGVEVTSAREHRLSFDEIFAILVAQSEEQR
                     AAEAADAAAALASGSASSDGTEAAA"
     gene            3237..4361
                     /locus_tag="VHM48_00820"
     CDS             3237..4361
                     /locus_tag="VHM48_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753971.1"
                     /translation="MSDDRDSFAGLRHASDDRAGIRRRRSGRGFAYRDPDGRPIRDAA
                     TIARIKAIAIPPAWTDVWICPWPNGHLQATGRDARARKQYRYHPRYRSRRDGAKFERL
                     IAFAKALPAIRERVERDLGRPGLPREKVVAAVVRLLELTLIRVGNDEYARLNRSFGLT
                     TFRDRHAAVEGTEITFRFRGKSGQQHQVGLRDRRLAGVVRRCRDLPGQELFQYEGADG
                     QPQDIASDDVNAYLAGIAPGITAKDFRTWAGTVLAYRALRSIGRGSTDREKQRNIAAA
                     IRATAENLGNTPAVARQAYVHPAVVDAYLDGRIRSALVAAAEDTDEAPGATDPDEERA
                     VVALLRARLREDAGRSRGRSPRRADRSTRGRGSRSGAVRA"
     gene            complement(4450..5652)
                     /locus_tag="VHM48_00825"
     CDS             complement(4450..5652)
                     /locus_tag="VHM48_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2753972.1"
                     /translation="MLDRQPSNQLNQAPAAESPAGPTQATTSRTRPPSVGLAASDLGR
                     VRAVIDALPDAVFVTESDGELRLTNPAADRLFAREPIHDRADLLARFEEIASSGVGRP
                     VKAPPDDAPGHVTVRPRDQPNRWFTLRTVALDPGRPGPGSEGNAEPSDAVPSSGQTVF
                     VLRDVTDSRDLRPVREAFLAILSHELRTPITTIYAGSSVLARHPTLSPPATRTLANDI
                     SAEAARLYDLVEDLLVLARLERRVLDPLDEPVLVQRAVDATIRTVEDRLGDAPIERRD
                     DARVPPVHGDATYVEQACRNLVMASIRCAGPEPGRQLVVEVRTHPDAGEVSVAILDRG
                     PQLAPDELARAFELPDSSDVGRLAAAGVGPFVCRHLVAAMGGRSWARNRPDGGLEMGF
                     ALRIDERS"
     gene            complement(5862..6068)
                     /locus_tag="VHM48_00830"
     CDS             complement(5862..6068)
                     /locus_tag="VHM48_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753973.1"
                     /translation="MGNEDTKADVKETMRNVDNKAKETWRKADGNESLSDKVADAGDD
                     VRDGLGNAGDEVRRHTDDKNDRSV"
     gene            6299..6658
                     /locus_tag="VHM48_00835"
     CDS             6299..6658
                     /locus_tag="VHM48_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753974.1"
                     /translation="MVSSTTSTTPSSRTDDDTDLAATAKGIASDVADRAVAAAQRLPD
                     AAAVTGAQLERANQIIQGETDEVLAVGTSLSLGLAMGLLLGGANRLLVVLALIPATAM
                     AFTLFDRHGGTRTIRPR"
     gene            complement(7478..7759)
                     /locus_tag="VHM48_00840"
     CDS             complement(7478..7759)
                     /locus_tag="VHM48_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX2753975.1"
                     /translation="MNIIAWIILGAIAGYLAGFLVKGDEGLGVIGHIVLGIVGALVGG
                     FLASVLFNTKPIDGAFDISSIVVATIGAIITVVVVNAVTGRGRTGRGAI"
     gene            complement(8357..8542)
                     /locus_tag="VHM48_00845"
     CDS             complement(8357..8542)
                     /locus_tag="VHM48_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753976.1"
                     /translation="MIKRILLALALAASLGGAVVACNTPAGTAGPTLTTPSTTPASIP
                     AASNVPSSDLQPSPSAS"
     gene            8956..9081
                     /locus_tag="VHM48_00850"
     CDS             8956..9081
                     /locus_tag="VHM48_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753977.1"
                     /translation="MIRQTTLPTTEPDVEDRSMRLVEYAMAFAAVLAAAVLAIIR"
     gene            9078..>9650
                     /locus_tag="VHM48_00855"
     CDS             9078..>9650
                     /locus_tag="VHM48_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2753978.1"
                     /translation="MTLRAILYDARGVDREVDLREEPSPDLDRDRLLWLDLDDRDVED
                     LARASAAIGLEPEGLRRLSREDRRARILRLPKSVVLTLGAVDPDDADARRHELDIVVG
                     ANHVITVHEGPLAAIDAFRAEIGHEDQLGRLDAAAFVAGLIDAVFAAFFQQIESIEQD
                     IDALDELALRARSEDSFLTRVLTLRRRIARL"
BASE COUNT         1483 a         3328 c         3463 g         1376 t
ORIGIN      
        1 ttcgagatcg acccgccgca catcgcggcc gccgcgcttg ctgagctggg gcgctgcggg
       61 gcgatggcac ccgacaaggt cgccaaggcg atggcgacgc tcgacgtgga cccggccaag
      121 ctggacccgg cggcgctctg aagcgcgagc gggcatcgtt ctctcgccca ggcgagcctc
      181 gcctgttgac gccggcgttg gcaaccaagg tcggcgattt cacgctcgat acggtgcccg
      241 gccgtggtgg cagaggcgac agccgcctga gcgcaagaga ttcgggcgtg gatcgatgcc
      301 aacaggcgac agccgcctga gcgcaagaga ttcgggcgcg gagagacgcg gaccggcgcc
      361 cgccctcgcc gaacgacgtc tcgtcacgcg gctgatgtgc gcgcccggcg cggcaatgcg
      421 cggccgccgc cggccgccca tgtcctggga agttgcccgg atactcgtcc acggactcgt
      481 aaaccaggtt gtcgagcgac ggacgcgacg tcccgcggcc ggcgtgccgt tgctcgtgca
      541 cgatccggag gccatcgacg ccgtccacgg cccgcgtcgg ctcctcggag cgacgatttc
      601 ccgcctgatg agtccgccgg tgactacccg gccaggtttg tcggccggag cagccgtcga
      661 cgggccggag tagccctcgc cggccagcca ccgtgccccg ggaccctagg cccgggcgac
      721 gacccgccgc agcagcagcc acgccccgcc gatataggcg accgacaacc cggccagcac
      781 ccagatctgc cagatgacga ccgtcccgcc gcggaacatg aagtcgccga acagccggat
      841 cgcgcccgcc accggcagga tgtagatcaa cgagcgaacc ggttcgctga actcgctgat
      901 cgcgaggacg aagccggaga agaacacgga cgcgagcagg accaggagcg tcagctggac
      961 agcctgcgcc tcggagtcgg agacggcggc gatcaccagg ccgagcccga gcgacgcggc
     1021 cacgagcagt cccgcgacca gggcgatcca accgggttga gcgaggaacg gaacattgaa
     1081 cccgacgacc agcagggcga tcgtcgcgaa ggcgaccgcg cccgcgaaca gcccgaacgc
     1141 gatgagcttg ccggtcacca gctcggccgt cgtgatcggt gagatccgga acagctcgaa
     1201 caggccgctg ttcttctccc ggacgaccga caacgcgatc agggtcaccg cgagatgctg
     1261 gaggatcagg gcgaggaccg ccggggcgaa gtactgaacg acggtcggct cggattgggc
     1321 gacgttgtcg atccacgcct cggtcggggc ggcgatcacg ttggccggga tcttgtagac
     1381 gtcgttgacg ccatggtcga tggcataccc cttgccctcg tcgacggcgt cctcgatgac
     1441 ctggcggttg accgcggcct cgagcccgag agcgacgaag accgcgtagt tgctctggac
     1501 cggatcggtc aggttcaccc ggacgtcgat ctgggagcgc aggccggcct cgaggttggc
     1561 ccggacgtcg gacggggcga cgaccacgac atcgacgagc tggcgattca gctgatccat
     1621 cgccgccgcc tcgtcgggcg tgacgagggc gatcctgagg ccgacgcccg ccacgtcctg
     1681 gtaactcttc gcgtcggtcg gaagtcccga atcggccggc aacacgacga tcgtctcgag
     1741 cggccggtgg aagccgctgt agccgagacc gaagatcgcc atgatcagga acggcccgag
     1801 gacgaggctg atcagcgctc ccggccggcg gacgatcgac acgagctcct tgcccacgat
     1861 cgacaggatg cgcgtgagca tcttgaacag gctcatgccg cggcctcggt tccgtcgctc
     1921 gacgcggacc cgctcgcgag ggcggccgcg gcatcggccg cctcggcggc ccgctgctct
     1981 tcgctttgtg cgacgaggat cgcgaagatc tcgtcgaagc tcaggcgatg ctcgcgggcg
     2041 gaggtgacct cgacgccggc ggccctgatc gtctcgacga cgaccggcag gaagctgccg
     2101 gcatcgtcga cgacgacccg cacatggcgc ggcccgtcct ggttgacctg ccggacgccg
     2161 gccgtattga ccaggaccga tgcgtcgaag agcgccgacg tctcgatgtc gaggaggtcg
     2221 ccgtccgtcg ccagacgacg aagctcgtcc ggcggcgcga gggcgatgat ccggccgttg
     2281 acgatcatcg cgacggcatc gcactgctcc gcctcgctga cgtgctgggt cgtgacgagg
     2341 agtgtccggc ccacgtcgcg gaggcgatgg agctcggtcc agatcgagcc gcgcagcagc
     2401 ggatcgaccc cggccgtcgg ctcgtcgagg atgatcaggt ccggctcgtg gacgagggcg
     2461 caggcgagct cgagacgccg ctgcattccg ccggacaggc ggccggcctg gcgtccccgg
     2521 gcatcccaca ggtcgacgag cttgaggacc tcgcggacgc gtcggcggcg gcggcgccag
     2581 agcatcccga acaggctcgc gacgaaatcg atgttctcgc ccgccgtcag gtcctcatac
     2641 agggtgaacg attgcggcat gtagccgatg cgttcgcgat cggcccggcg gaaggccttc
     2701 gggtggcggc ccatgacgag cacctcgccg ctcgtcggtg cgatcgctcc ggtcagcatc
     2761 cggatcgtcg tggtcttgcc ggcacccgag ggcccaatga tcccgaggat cgttccgcgg
     2821 cggaccttca ggtcgacacc gctgaggacc tcgcgatcat cgaaccggcg cgagacctgg
     2881 tgcatcgcga cgatgacgtc gcggtccgct tcgaggccgc cgtcgggcgg tcgttcgttg
     2941 gtcggctcga tcggaggtgg atcggaagcc atgcgtcctc ctgggggtct ggtgggcgat
     3001 ggcccgcgct gggcacccgc attctgtcga tcgatccatc ccgccgacgt gtctgcggcc
     3061 cgccgcggcg atacgaatgc atgacgaccc atccggcggc ccgaccgacg acggtcgaac
     3121 gcgggctcgc ggtcggaacc ctgcaacacg ggtggaaccg tccgggccgg gcaacatcgc
     3181 gtgcgcgaga cgcgtcatcg acgatgctcc caggcgatgg acctggacag cctgccgtga
     3241 gcgacgatcg ggacagcttc gccgggctcc gtcacgccag cgacgatcgg gccggcatcc
     3301 gccgccgccg gtccggccgc ggcttcgcct atcgcgaccc cgacggccgg ccgatccgcg
     3361 acgcggcgac catcgcccgg atcaaggcga tcgccatccc gccggcgtgg accgacgtgt
     3421 ggatctgccc atggccgaac ggccacctcc aggcgacggg ccgcgacgcc cgcgctcgca
     3481 agcaataccg ctaccacccg cgctaccgat cccgccgcga cggggcgaag ttcgagcgcc
     3541 tcatcgcgtt cgcgaaggcc ctgcccgcga tccgcgagcg ggtcgagcgc gatctcggcc
     3601 ggcccgggct gccgcgcgag aaggtcgtcg cggccgtcgt ccggctgctg gagctcacgc
     3661 tgatccgggt cggcaacgac gaatacgcgc ggctcaaccg gtcgttcggg ctgacgacgt
     3721 tccgcgaccg gcatgccgcg gtcgagggga cggagatcac gttccggttc cggggcaagt
     3781 ccggccagca gcatcaggtc ggcctgcgcg accggcggct cgccggcgtc gtccgccgct
     3841 gtcgggacct gcccggccag gagttgttcc agtacgaggg cgccgacggc cagcctcagg
     3901 acatcgcctc cgacgacgtc aacgcctacc tcgccgggat cgcgcccggg atcacggcca
     3961 aggacttccg gacgtgggcc gggaccgtcc tcgcctatcg ggcgctgcga tcgatcggca
     4021 ggggctcgac cgaccgggag aagcagcgca acatcgccgc ggccatccgc gcgacggccg
     4081 aaaacctcgg caacacgccg gcggtcgccc gccaggccta tgtccatccg gcggtggtcg
     4141 acgcctatct cgacggccgg atccggtcgg cgctcgtggc ggccgccgag gacaccgacg
     4201 aggcgccggg cgcgaccgat ccggatgagg agcgggccgt cgtcgccctc ctccgagccc
     4261 ggctccgcga ggacgcgggc cgatcccgcg gacgatctcc gcgccgcgcc gacaggtcga
     4321 cgcgtggccg cgggagccga tcgggagcgg tgcgggcctg agcgacggtc ttcctcgccg
     4381 ggatcttcgg catcgtcggg cggttcgcca gccgcgtcct gacgactcgc gtctcgctga
     4441 agccgatcct cagctgcgct cgtcgatccg cagcgcgaac cccatctcga gcccgccgtc
     4501 gggccggttg cgcgcccacg aacggccacc catcgccgcc acgaggtgac ggcagacgaa
     4561 cgggccgacg cccgccgccg cgagcctgcc gacgtcggat gaatccggca gctcgaacgc
     4621 ccgggcgagc tcgtcgggcg cgagctgcgg gccccggtcg aggatcgcga ccgacacctc
     4681 gccagcgtcc ggatgggtgc ggacctcgac gacgagctgg cggccgggtt cgggtccggc
     4741 gcagcggatc gaggccatga cgaggttgcg gcaggcctgc tcgacatagg tcgcatcgcc
     4801 gtgaactggc ggaacgcggg cgtcgtcgcg acgctcgatc ggcgcgtcac ccaggcggtc
     4861 ctcgaccgtc cggatcgtcg cgtccacggc acgctggacg aggacgggct cgtcgagcgg
     4921 atcgagcacg cggcgctcca gcctggcgag cacgagcagg tcctcgacga gatcgtagag
     4981 gcgggcggcc tcggcgctga tgtcgttggc gagcgtccgg gtcgcaggag gagagagggt
     5041 cgggtgccgg gcgaggaccg aggagcccgc gtagatcgtg gtgatcggcg tccgcagctc
     5101 gtgcgacagg atcgcgagga acgcctcccg gacgggccgc agatcgcggc tgtcggtgac
     5161 gtcgcggagg acgaagaccg tctggccgga cgaaggcacg gcatcgctcg gttcggcgtt
     5221 gccttcggat cccgggcctg gccggccggg atcgagggcc acggttcgca gggtgaacca
     5281 gcggttgggt tggtcgcgcg ggcggacggt gacgtggccc ggggcgtcgt ccggcggggc
     5341 cttgacgggc cggcccacgc ctgacgacgc gatctcctcg aacctcgcga ggaggtccgc
     5401 ccgatcgtgg atcggttcac gggcgaacaa ccgatcggcg gccgggttcg tcagtcgaag
     5461 ctcgccgtcg gactcggtga cgaacacggc gtcgggcagg gcatcgatca ccgcccggac
     5521 ccggccgaga tcggaagcgg cgagaccgac cgatggcggc cgggtgcggg acgtcgtggc
     5581 ctgggtcgga ccggccggcg actcggccgc cggcgcctgg ttaagctggt tgctgggttg
     5641 tcgatcgagc atcgtgctca aggatcgcgg gccgggaccg acgggcggac tgggcctgag
     5701 cggctgtgcg ttgcggctcc atcgcaggcg cgcggcggcc gatcgtggag gcggcgggcc
     5761 ctggcgaccc tgcgcggacg ctcagcgccc ccgccggatt cgacgggggc gttgcgacgc
     5821 agcgcgagcc tgactgtgga gcggcgcgag cttgacgccg atcagacgga ccggtcgttc
     5881 ttgtcgtcgg tgtggcggcg aacctcgtcg cctgcgttgc cgaggccgtc gcggacgtcg
     5941 tcaccggcgt ccgcgacctt gtcggacagg ctctcgttgc cgtcggcctt gcgccatgtc
     6001 tccttggcct tgttgtcgac gttgcgcatc gtctccttga cgtcggcctt cgtgtcctcg
     6061 ttgcccatgt caggaacctc cgggtggatc ggagccggtt gtccggcccg tttcgtagaa
     6121 cctaaccggg tggacattgc gccggcctcg gcgccggccg ggccagatca caacgcgctt
     6181 gcagaccgcg gctgtcacgt gcctgcaacc gggctcgtca acgcgaggtg gttgctgcgg
     6241 cgccgcggcc cgtaccgtca gaggccgtca acccgaactc cctggaggta ttcaccacat
     6301 ggtcagcagc acgacctcga cgactccgag ctcacgaacc gatgacgaca cggatctcgc
     6361 ggcaaccgcg aaaggcatcg cgagcgatgt cgcggaccgg gcagtcgccg ccgcccagcg
     6421 cctgcccgat gccgcagcgg tgacgggcgc ccagctcgag cgagccaacc agatcatcca
     6481 gggcgaaacc gacgaggtcc tcgccgtcgg cacatcgctc tcgctcggcc tggcgatggg
     6541 cctgctgctc ggcggggcga accgattgct ggtggtcctc gccctgatcc cggcaacggc
     6601 aatggccttc accctgttcg atcgccacgg cggaacgcgg acgatccgcc cgcgctgagg
     6661 gcgcggctcg gcggcctcgg cagccggcct cgccctgacc cggacaccac gaagccggtc
     6721 ggctcgctgc cgaccggcct ctgcgtgtcc tcggacgtcg gttcgggcct tcgatccgct
     6781 cctgggccca ggaaccgtgg atttcggacc cggacgagcg gaacgcccgg aggagggggc
     6841 tccgggcgtc cgcgggatcg gggtgtgaat gcgccccgtc acaagcgcct ccacgaggga
     6901 tgttcagttc gacctggcgg cgcaccgccg agcgtgatag cgaatctacg tggcaggccg
     6961 cgcagggagt cttgcgtgga tcgtgcgcga cattgcatcc gcattgcgac cgcgagtggc
     7021 tggggcgaag ccgtcgtggc cgcggcccag acggccgatg tcggaggttg agaaggcggg
     7081 ctcccggacc cccagtggtc ggaagcccgc ggaaccaagt gtcacggtcg tccactgccg
     7141 cgcgcctctc acaatcggcc cggggagttg cagcggtctc gtgcggacgt tgcaacgaag
     7201 cgtcgtgacc cccgtggtgg cggatggcgt cgggtcaggc catcgcgtcg ggtcaggcca
     7261 tcgcgcggac ggactgcacg ccttccatga actcgggaaa cgtctcgaac tgggtccact
     7321 ggtcgtacgc ggtccggacc gggacctgga catcgacggc gtttcagcca aggagatccc
     7381 gtgtcgaaga tcatgggggg aggtaatcgg aacgcccacc tcgggaaggt gggcgtccga
     7441 tggcttcggc ggagcaagct ccgctgagct cggcgggtca gatcgctccg cgacccgtcc
     7501 ggccgcggcc ggtgaccgcg ttcaccacga cgaccgtgat gatcgcgccg atcgtggcga
     7561 ccacgatcga gctgatgtca aacgcgccgt cgatcggctt ggtgttgaac aggacgctgg
     7621 caaggaagcc gccgacgagg gcgccgacaa tgccgagcac gatgtggccg atgacgccga
     7681 gaccttcgtc acccttgacg aggaagccgg ccagataacc ggcgatggcg ccgagaatga
     7741 tccaggcgat gatgttcatt tgggtcagga cctccatccc tgatgtctga tttcgtcccg
     7801 aggtcgggac cgagacggat catcggcggg cgattgcgca agatcgacaa cggcacgggc
     7861 cttctccatt gcatccgtct gtcagccgcc cacgtggctc gccatccgtc atcgtgaggc
     7921 gcccattcga cgggacggcg gtcggggcga cggcaggctg cagactcgtg atcgagggcg
     7981 cattgcgcgt gggccgtggg gcggtcgagg cgacgggcga atgggtcgcc ggaggcgtcg
     8041 cggccggacc ggacagacag ttgcggggct gtcgaggtcg tcgtctctcc cagcgccgca
     8101 ttgtctgaca gtcggatccg ggaccgatcc cggatccgac cagggtcccc gagcgccgcg
     8161 tattcgaagg gtcgcctccc gacaacgccc ggagccggtt cgaaggatcg ccttcgatgc
     8221 gttgcaccca tcttctgaaa ccgaaggggc ggattgcacg cacgttgcac cgcccccgtt
     8281 gcggcgaaga aacctgcgat ggaatcaaga aacgccccgg gtccgaagac ccggggcgtt
     8341 ctcgcgtgcg gggggactag gaggcgctcg gcgagggctg gaggtcgctc gacggaacgt
     8401 tggaagcggc cgggatcgag gccggagtcg tgctcggggt ggtcagggtc gggcccgcgg
     8461 tgccggccgg cgtattgcag gcgacgacgg cgccaccgag agacgcggcg agggcaaggg
     8521 cgagcaggat tcgcttgatc atgagatggg gattcctccg ttgccagggc accggcgggt
     8581 ccgatgcctc gcgtggccgg gagcgaccag cgcccagacg ataggcagcc ctaccgaacg
     8641 aacgcccggc gcgcgacgcg cgaactcttg cattggtctg ccagtcgaag ccgtccgcgc
     8701 ggtcaattcc acgccgccgg tccacgccgc ccgggacgcc cgtcctggtg cgccgcgccg
     8761 gctcaccagg ccccggccgc gggagtgcaa cgagcgtgat aggggcgggc cgggcggacg
     8821 tctgtgtctg cgaccggccg ccgtccatgc tgcctcatcg caacgacgcc gccccatccc
     8881 ggtggcgtcg tcgaccaggc cagagccgcc caccggtcga ttgccccggg accgcgacca
     8941 caggagtcac gatcgatgat ccggcagacg acgcttccga ccacggagcc agacgtcgaa
     9001 gaccgttcga tgcgtctcgt cgagtatgcg atggcgttcg ccgcggtcct ggccgccgcg
     9061 gtcctggcca tcattcgttg accctccgcg ccatcctgta cgacgcccgg ggcgtcgatc
     9121 gcgaggtcga cctgcgggag gaaccatcgc cggacctcga tcgcgaccgc ctcctctggc
     9181 tcgacctcga cgatcgcgat gtcgaggacc tcgcccgcgc ctccgcggcc atcggcctcg
     9241 agcctgaagg gctccgtcga ctgtcgcgcg aggatcgccg ggcgcggatt ctccgtctcc
     9301 ccaagagcgt cgtcctgacc ctcggcgcgg tcgatccgga cgacgccgac gcccgccggc
     9361 acgagctcga catcgtcgtc ggcgcgaacc acgtcatcac ggtccacgag gggccgctgg
     9421 ccgcgatcga cgcgtttcgg gccgagatcg ggcacgagga ccagctcggc cggctcgacg
     9481 ctgccgcctt cgtcgccggc ctgatcgacg cggtcttcgc ggcattcttc cagcagatcg
     9541 aatcgatcga acaggacatc gatgccctcg acgagctcgc gctccgggct cgaagcgagg
     9601 actccttcct gacgagggtt ctgaccctcc gccggcggat cgcccggcta
//
LOCUS       DATAIW010000023        15934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_119507, whole genome shotgun sequence.
ACCESSION   DATAIW010000023 DATAIW010000000
VERSION     DATAIW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119507"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..752
                     /locus_tag="VHM48_00860"
     CDS             141..752
                     /locus_tag="VHM48_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753979.1"
                     /translation="MSDELVLVVPTAAVLAELGGGRAWLGIREAGEDDLAALIRRDGV
                     FRARSEMEADPAWKQVIPYPVLRDGGAWYLMRRTKAGSDARLHDRYSIGVGGHVNPED
                     GGLDGDLAAALRREWHEELVVDFLPAFRFIGLLNDDTTAVGQVHLGLVYEGDAAGRAV
                     AIRETDKLSGAFVTSAEVAAVSDRLETWSRLAFEFIEAAAALR"
     gene            767..2128
                     /locus_tag="VHM48_00865"
     CDS             767..2128
                     /locus_tag="VHM48_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX2753980.1"
                     /translation="MRQQTSAIQPIRIVAALFLVLGLIGLAGTARAAGKTVYVLPTTG
                     IVDEGMSHYLADGIAQAERDGAAAVVIKLNTPGGGLFATNDIVGTILEAKVPVIVWVA
                     PAGGFAASAGTFITLAANVALMAPGTSIGAASPVTGEGGDITGTEGDKVRNDAIAKIT
                     AIAQARHRNVDWAVQAVSSARSSPASEAVQLGVVDGIASTLDEVLASATGREIEVAGS
                     TVTLDLVGAATAEIDMSPFLAFIRLLSDPTIAFLLFSAGSAGLLAELWSPNFVTGILG
                     ALLIILAFIGLGALPLNVGGVLLIILAMVLFGLELTVTSHGLLGFGGLVCFALGASAL
                     FTAPVDPFAPLVRVAAPVVATTTVTAAIFLAFVVWAAIRSRDMLASEGTVGRAIALAP
                     GTEAIVRRPIEPLGSIHIAGEEWSARSVDGRPLDRGTAVRVVGTDGLTAIVERVASSS
                     STS"
     gene            2145..3044
                     /locus_tag="VHM48_00870"
     CDS             2145..3044
                     /locus_tag="VHM48_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2753981.1"
                     /translation="MEAPIIAATAAFIVVVLLVIVYLSIRVVQQYEKMVVFRLGKTNE
                     SMIRDPGLRFLVPVIDRPVKVDVREQFIEVPSQTTITRDNAPINVDFLIYWRISDPLK
                     SVVNVVNFPGALQGVATTTLRAVIGDILLDEVLSKREQINEVLRVKLDEVTERWGGKV
                     TTVEIREITPPRDVQDAMNRQLSAERTRRAVITESEGSRQAAINVAEGQKQAEILKAE
                     GDRQAAILRAEGFSEALTRIYGAASGIDAKTMALQYLEALKALGASPSTKFVIPTEFT
                     RLLEPIQGFVERGMGSEAGPRAK"
     gene            3041..4057
                     /locus_tag="VHM48_00875"
     CDS             3041..4057
                     /locus_tag="VHM48_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2753982.1"
                     /translation="MIGRAATRLLDAPRVGPDEAGLKGAVDALGGEVIRLRSRDGLKL
                     TARWLPAESGPVPGDDGPPWQPDPYEAILLLHGWTGSVAPDLVELGPTLRRTAGVLGL
                     DFRGHGGSDDAPTTFGLHEIEDVAGALTWLGERGIRRVALVGSSMGGVVAIASIVVLG
                     DGSLAQADLDPTAPASTVEAPGPRIAAAVAESVAPDLRIPIANRMSLPIGGVLRRAFA
                     GGMFRLAGQRVGGDLRATEPGRIVGLVEPVPLLLIHGGADRTVRVADGRRLAARAGPS
                     AEHWEVPGAEHGQARRAAPALWDERVSRFLRQAFFGSREVVPIIHASGEPTADSGFRG
                     LEGD"
     gene            4057..5226
                     /locus_tag="VHM48_00880"
     CDS             4057..5226
                     /locus_tag="VHM48_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2753983.1"
                     /translation="MAAKILVVDDDPNVQRLLQYTLKQEGYEVVIASDGAEGFRLWGA
                     EAPDLILLDVMLPKLDGYQVATKIRTEEGTSGHVPIIMLTAEREVEQKVRGLRAGADD
                     YLIKPFHPAELMARIKSLLARFAPHETLVGRPPLGRVLAFYGSKGGVGTTTIAINAAI
                     ALHRELGRKVALVDGNLQFGDHRVFLDLGLDKKSIVDIVTAPNIDQDLVRQVMVKHDS
                     GVDLLLAPPSPETAELVTQDHMPIILEQLRGLYDYVLIDIDKRLDDVNLGILEAADTM
                     FVVMTADLSCLKNVRLVLETIGHLGYSAQKVQLVLNRSNAFTGISVKNAEGALKRTID
                     HQVVNEYRGAISALNSGAPFMFTKADSLLGRSLLEFARVVDKTPIAAATPALARG"
     gene            5453..7060
                     /locus_tag="VHM48_00885"
     CDS             5453..7060
                     /locus_tag="VHM48_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX2753984.1"
                     /translation="MLATLLSSTLVGLDGRIIRVEVDVAPGLPGFTIVGLADAALQEA
                     RERVRGAIRNAGFSHPPRRITVNLAPADLRKAGASLDLAIAMGILLGSEQVVAAGGRV
                     ALVGELSLGGEVSRVPGILPMTAALRRRGIGRVIVPADSAAEAGLVAGIDIVGVRTLR
                     EAADALRRRGRRTTRLAPRRVELAEPGSGEPGSDEPESGEPLRSGIGAEVASPVDATP
                     DLAEVRGQLEARRALEIALAGGHGLLLVGPPGTGKTLLARTIRGLLPPLDDAEALAAT
                     IVASVAGEGPIHELRRSAPVRAPHHTLSYAAMVGGGPRMSPGEVTLADHGALFLDELP
                     EFGRDVLEALRQPLEDGFVSIARVGRATRFPARFLLVAAMNPCPCGYAGDPSGRCRCP
                     IGTPERYAGRISGPLRDRIDLWVTMARVPPEALIAGRDPESSAVVAARIAVARGVQAR
                     RTMRLNGRLAGRALRAACDLSPAATARAVALADLESLSGRGTERILRVARTIADLAGS
                     ARVEPDHLDEAARFRSPASRLDAREAS"
     gene            7075..8442
                     /gene="dprA"
                     /locus_tag="VHM48_00890"
     CDS             7075..8442
                     /gene="dprA"
                     /locus_tag="VHM48_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX2753985.1"
                     /translation="MDRPQLRLVTSGDPKASPGARGGAGGSTAVPDEREAWAVLLSVH
                     GLGPAGFGALLAAYGSGRAILAAASLRGAAPRFARIAADGDGRPAFREPVGDGIVAAA
                     AALDDRLAVLRASLLRIVTLDDAAYPPRLRAIDLPPPVLLVRGEPATLSSAHAIAIVG
                     TRRPTERGRLIAARIAGALAKAGAVVVSGLAVGIDGAAHAAVEAEGRPTVAVLGSGHG
                     RLYPRAHQRLATGIVGAGGAVVSELWPDQPPSAHTFPQRNRVISGLADATIVVEAGLK
                     SGALITAKWALEQGRDLFLVPGPIDETRSAGCLHWLREFPGEARIVATVPELIADLGL
                     LDQADEPASVGHGPGDDGTGPSDPPHRPPRPGLDAVLVELGPTARDVGAALVAGHGSL
                     DELVAVTELEPATVLGAITLLELRGLATTTYGRYRAAGQLASATAPEVRSGSSGRLPV
                     RRGPC"
     gene            8531..10204
                     /locus_tag="VHM48_00895"
     CDS             8531..10204
                     /locus_tag="VHM48_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2753986.1"
                     /translation="MRKLAAAALAVPVLAIVYLPLLARRSVAARVGLVASVGVIVLVA
                     ALGLSRPVVTTASQPAPPITALADSAFRSIDAATDLHAAVDITFSEAMDPRSVAASLT
                     VTPATATSISWNAARTVLTVRPTSSWAPGTYHTITVAAGTLAAGGRPMSTAVRAAFIT
                     RAPTSGRIAPTLRAGNATSLATAFRITFDHPVAIDEVATALKISPTVDGKLVADSGRP
                     DVIATSALGFTFTPAAVLAGGTAYRLTLGPLVDTDGAAVGSVETVAVRTSAAPRIVRF
                     RPADGTKKVERRAALSVRFSDPMDHVSTRAAFTVTANGKPVHGTYRFAESSTVLVFQP
                     VSALPAGAKVVISIGATATSAQGVQLARASSATLTTVPKRKAAPRTAPTVSRSSSGSG
                     SSGSSSSGGGAVGGGSWGAVETYYLRLMNCTRTGGWVTSTGSCSSPGGRNVAPLKLDA
                     GISSHVSRPYAKKLAVGGWCDHFIGGNPGDRLRSAGYTSYIWAENLGCRSGNPYSAVL
                     GSHLFFQSEKPYSGGHYVNLMNSAYDRVGIGVWVSGGRVRLVIDFYHPR"
     gene            10310..10645
                     /locus_tag="VHM48_00900"
     CDS             10310..10645
                     /locus_tag="VHM48_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753987.1"
                     /translation="MIRNVVIHISNEQPLLADLYEMPTALDVSLVCTNMRMLDGKKPI
                     FIDHSNSVFVFPYLNIRFIEILVGEATGMPDLPLDGRDVAAGETTDDTESEADLELDE
                     DFLRRIRDV"
     gene            10732..11355
                     /locus_tag="VHM48_00905"
     CDS             10732..11355
                     /locus_tag="VHM48_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2753988.1"
                     /translation="MIRLAIVDDHPAIAVALAAAIRDRADLELVGSATATADALALVE
                     RTAPDVVLCDLWLDGEPGGLDVLASLTASDRRDGPRVLVLSGFDQPSFLRAAFEGGAA
                     GYVSKSSPVESIVDAVLAVARGETCFPPVTLRALRDAPRRPSVREMSAIRLLARGASN
                     DEIAVGLGISIKTVESHLRRLFGRYGVLSRTELAMLAVREGWLGIGP"
     gene            11352..12665
                     /locus_tag="VHM48_00910"
     CDS             11352..12665
                     /locus_tag="VHM48_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2753989.1"
                     /translation="MRLAVEQRGVRWSWSALDLAIAGFSLTAVATTVLSLGQPAITPI
                     GPIPILLALGAFCVAALARRPAPSLAWIATIGGSFAASSIPIDQARAADPGQLGVGPW
                     LGLAVPASVGAIVTLGIAARYATRADRRLGRIAVPVSVGLFAWLVVACATTIGLIGTG
                     QARADPEFTWIDVATAPIAGFVRFVVVVTTLGVLADVRAGYERARERLAGPPTTTAAA
                     ERAWALAVATIRELVPGQSAAEEASLAAERTRLAGDLHATVLPGLRRAIAEAESGGDP
                     DVLARQLRTVDLELERLMADRWPVVLEAFGLVAALEDLAERIEADTSLGVQIDVERAG
                     DRPPPTIERAAWRVAQLAVDNAARHAEASLVTVTVSVDVERLVLAIADDGRGFDPDTA
                     GAVRAGARGLADATRRAAAVGAAIRIEPRPGGGTTVTFDWTAHRA"
     gene            12811..13005
                     /locus_tag="VHM48_00915"
     CDS             12811..13005
                     /locus_tag="VHM48_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753990.1"
                     /translation="MTLLRRIRALRFVEPDRDRSTDRAGQGLAEYALILALIAIVAII
                     ALIFLGGQISSILSHVGTSI"
     gene            13173..13526
                     /locus_tag="VHM48_00920"
     CDS             13173..13526
                     /locus_tag="VHM48_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753991.1"
                     /translation="MGLATGGCNVIAGPPEILDGLSIGADAGCGACDAPDTSANCGAC
                     ESIATLAVRHVDETWPDHPEIGTMSFHRESWYPGENGERILHTRSGSLVVAVVTFVDG
                     TRHAASVYCGVGGCR"
     gene            13629..>15934
                     /gene="topA"
                     /locus_tag="VHM48_00925"
     CDS             13629..>15934
                     /gene="topA"
                     /locus_tag="VHM48_00925"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX2753992.1"
                     /translation="MSKNLVIVESPAKARTIERYLGADYKVLASYGHVRDLPENPGKG
                     KFGVDVDHDFAPEYVVSDDRRKQVAEITKAAKSAEHVFLATDLDREGEAIAWHVAEAA
                     HVPASKTQRVTFSEITEGAIREAFAHPRGIDTNLVDAQQTRRIVDRLVGYTLSPLLSR
                     KVRSGLSAGRVQSVAVRLVVEREREITAFTAREYWTLEVLLATVDGAVFAAELVRIDG
                     AALDVGDEETAARHAAALRDLKPRVTKVGTRKQSRSPAPPFTTSTLQQEASRKLGFSP
                     KRTMSVAQRLYEGADTPDGHVGLITYMRTDSTAIAGVAMGEARDVIRARFGDPYTMPK
                     GRVYRTKSKGAQEAHESIRPTSFRRDPDSLAGTLANDELRLYRLIWQRAIASQMAAKE
                     LETTTVDLVAGPYELRASATKTLFDGFSAVYTEGRDDGAAEDVEAERALPPVNEGDLT
                     TVREVTPAQHFTEPPPRFTEATLIKALEEHGIGRPSTYAATISTILDRGYVRVEERRL
                     HPELIGNIVTDWLVEHFGEYVDVAFTARMEEELDEVARGERPWVPFLRAFYDPLAELV
                     AEKRKTTTRPTEPTDEVCSEGHPMVIRLGRNGRFLACSLFPEHKESRPLPGEEPPPQA
                     GEGEVCPECGEGTLVGKRGRFGPFVGCSRYPDCTFIKKEGPPPPDPLPFKVTCPKNND
                     GQLIPRRARRTGNVFYGCSNYPKCDYTTNFEPVGALHDTDDGPVAKHGDAGICLRCGA
                     AIPLAEGVDVAGVKLEGGPPNPEALAKPA"
BASE COUNT         2189 a         5803 c         5652 g         2290 t
ORIGIN      
        1 gtgatcgacg aatggctcgt gctgctcgcc gagggcgccg ccgacccgga gggcctccgg
       61 gcgcggctga tggccgcgcg cgatctcgcg atggcatccg atcccggcac cggaacgacc
      121 gccgcccacg gggaggcccg atgagcgacg agctggtcct cgtcgttccg acggccgcgg
      181 tgctcgccga gctgggcggc ggacgggcct ggctcgggat ccgcgaggcc ggcgaggacg
      241 acctcgccgc gctcatccgg cgcgacggcg tcttccgggc ccgatcggag atggaggcgg
      301 atcccgcctg gaagcaggtg atcccgtatc cggtcctgcg cgacggcggc gcctggtacc
      361 tgatgcgccg tacgaaggcc ggcagcgacg ctcgcctcca cgatcggtac tcgatcggtg
      421 tcggtggcca cgtcaacccc gaggacggcg ggctcgacgg agacctggcg gcagcgctcc
      481 ggcgcgaatg gcacgaggag ctggtcgtcg acttcctccc ggccttccga ttcatcggcc
      541 tgctcaacga cgatacgacc gcggtcggcc aggtccatct cgggctggtc tacgagggcg
      601 acgcggccgg ccgggcggtc gcgatccggg agaccgacaa gctcagcggc gcgttcgtga
      661 catcggcgga ggtcgcggcg gtcagcgatc ggctcgaaac gtggagccga ctggccttcg
      721 agttcatcga ggccgccgcc gcgctacgat gaccctggag gtcgtcgtga gacagcagac
      781 gtccgcgatt cagccgatcc gcatcgtcgc ggcgttgttc ctggtgctcg gactgatcgg
      841 cctcgccggt acggcccggg cggccggcaa gacggtgtac gtcctgccga cgaccgggat
      901 cgtcgacgag ggcatgtccc actacctcgc cgacggcatt gcccaggcgg aacgcgacgg
      961 cgccgccgcg gtcgtcatca agctgaacac gccgggcggc ggcctcttcg cgaccaacga
     1021 catcgtcggg acgatcctcg aggccaaggt cccggtcatc gtctgggtcg ctccggccgg
     1081 cggcttcgcg gcaagcgcgg ggacgttcat caccctcgcc gccaacgtgg cgctgatggc
     1141 gcccggaacg agcatcggcg cggcgtcgcc ggtcaccggc gaaggtggcg atatcaccgg
     1201 cacggagggc gacaaggtcc gcaacgacgc gatcgccaag atcacggcga tcgctcaggc
     1261 gcgccatcgg aacgtcgact gggccgtcca ggccgtgtcg agcgctcgat cgtcgccggc
     1321 cagcgaggcg gttcagctcg gggtggtcga tgggattgcc tcgaccctcg acgaggtgct
     1381 ggcgtccgcc accgggcgcg agatcgaggt cgcaggatcg acagtcacgc tcgatctggt
     1441 cggcgcggcg acggccgaga tcgacatgag cccgttcctg gccttcatcc gtctcctgtc
     1501 ggatccgacg atcgcgttcc tcctgttcag tgccggctcg gcggggctgc tggccgagct
     1561 ctggagcccc aacttcgtga cggggatcct cggcgcgctg ctgatcatcc tcgccttcat
     1621 cggcctcggg gcgctgccgc tcaacgtcgg cggcgtgctc ctgatcatcc tggccatggt
     1681 cctgttcggg ctggaactga cggtgacgag ccatggcctc ctcggcttcg gcggcctcgt
     1741 ctgcttcgcc ctgggcgcct cggccctctt caccgcgccc gtcgacccgt tcgccccgct
     1801 ggtcagagtc gccgcaccgg tcgtcgcgac gacgaccgtg accgcggcga tcttcctggc
     1861 gttcgtcgtg tgggcggcga tccggagtcg tgacatgctg gcctcggagg gtaccgttgg
     1921 acgggcgatt gccctggccc cggggaccga agccatcgtc cgccgtccca tcgagcccct
     1981 cggctcgatc catatcgccg gcgaggagtg gtcggcacgg agcgttgacg gccggcccct
     2041 ggatcgcggg acggccgtgc gcgtggtcgg gacggacggc ctcaccgcga tcgtcgagcg
     2101 cgtcgcgtca tcgtcgtcaa cgtcctgatc gaacggaggt cctcgtggaa gcccccatca
     2161 tcgcggcaac ggcagccttc atcgtcgtcg tcctgctggt catcgtgtat ctctcgatcc
     2221 gggtggtcca gcagtacgag aagatggtgg tcttccggct cggcaagacg aacgagtcga
     2281 tgatccgcga cccgggtctg cggttcctcg tcccggtcat cgaccgaccc gtgaaggtcg
     2341 acgtccgcga gcagttcatc gaggtcccga gccagacgac gatcacccgg gacaacgcac
     2401 cgatcaacgt cgacttcctg atctactggc ggatcagcga tccgctcaag agcgtcgtca
     2461 acgtcgtcaa cttcccgggc gccctgcagg gtgttgcgac gaccaccctc cgggcggtca
     2521 tcggcgacat cctcctcgac gaggtgctgt ccaagcgcga gcagatcaac gaggtgctcc
     2581 gggtcaagct cgacgaggtc accgagcgct ggggcggcaa ggtcacgacg gtggagattc
     2641 gcgagatcac gccgccgcgc gacgtccagg acgcgatgaa ccggcagctc tccgcggagc
     2701 ggacccgacg ggcggtcatc acggagtccg aggggtcgcg ccaggcggcg atcaacgtcg
     2761 ccgagggcca gaaacaggcc gagatcctca aggccgaggg cgaccgccag gcggcgatcc
     2821 tccgggccga ggggttcagc gaggcgctca cccggatcta cggcgcggcc tccggcatcg
     2881 acgccaagac gatggccctc cagtacctcg aggcgctcaa ggcgctcggg gcaagcccgt
     2941 cgacgaagtt cgtcatcccg accgagttca cccgcctgct cgagccgatc cagggcttcg
     3001 tcgagcgggg catgggcagc gaggccgggc cgcgggccaa gtgatcgggc gggccgcgac
     3061 gagactcctc gacgcgcccc gcgtcgggcc ggacgaggcc ggcctcaagg gcgcggtcga
     3121 cgcgctcggc ggtgaggtca tccgcctccg ctcccgggac ggcctgaagc tgaccgcgcg
     3181 ctggctgccg gccgagagcg gtccggtccc cggcgacgac ggaccgccgt ggcagccgga
     3241 cccatatgag gcgatcctgc tcctccatgg ctggaccggc tccgtcgcgc ccgatctcgt
     3301 cgagctcggt ccgacactgc gccggaccgc gggcgtcctg gggctcgact tccgcggcca
     3361 cggcggctcg gacgatgcgc cgacgacctt cgggctgcac gagatcgagg acgtcgccgg
     3421 cgccctcacg tggctcggcg aacggggaat ccggcgcgtc gcgctcgtcg gctcgtcgat
     3481 gggcggcgtc gtggcgattg cctcgatcgt cgtcctcggc gatggctcgt tggcccaggc
     3541 cgatctcgac cccacggctc ccgcctcgac cgtcgaggcg cccggaccgc ggatcgcggc
     3601 cgcggtggcc gaatcggtcg ccccggatct ccggatcccg atcgccaacc ggatgtcgct
     3661 gccgatcggc ggcgtcctgc ggcgggcgtt cgccggcggg atgttccgcc ttgccgggca
     3721 acgcgtcggc ggcgacctcc gggcgaccga gcccggccgg atcgtcggtc tcgtcgagcc
     3781 ggtccccctg ctcctgatcc acggcggcgc cgatcgaacc gtccgcgtgg ccgacgggcg
     3841 gcggcttgcg gcgcgggccg ggccgtcggc ggagcattgg gaggtgccgg gcgcggagca
     3901 cggccaggca cgccgggcgg ctccggcgct atgggacgaa agagtgagcc gattcctccg
     3961 tcaggcgttc ttcggcagcc gcgaggtggt gcctataatc cacgcgtcgg gggagccgac
     4021 agccgattcc ggcttccggg gactggaagg agactgatgg cggccaagat cctcgtcgtc
     4081 gatgacgacc cgaacgtcca gcgcctgctg caatacacgc tgaagcagga aggctacgag
     4141 gtcgtcatcg cgtcggatgg cgccgaagga ttccgtctgt ggggtgccga ggcacccgac
     4201 ctgatcctgc tcgacgtcat gctgcccaag ctcgacgggt accaggtcgc gacgaagatc
     4261 cggaccgagg aaggcacgtc cgggcacgtc ccgatcatca tgctgaccgc cgagcgggag
     4321 gtcgagcaga aggtccgcgg cctgcgcgcc ggcgccgacg actacctgat caagccgttc
     4381 cacccggccg agctgatggc ccggatcaag agcctcctgg cccggttcgc gccgcacgag
     4441 acactcgtcg ggcgaccgcc cctcggacgg gtgctggcgt tctacgggtc caagggtggc
     4501 gtcgggacga cgacgatcgc gatcaacgcc gcgatcgcgc tccaccgcga gctgggccgg
     4561 aaggtcgccc tcgtcgacgg caacctccag ttcggcgacc accgcgtctt cctcgatctc
     4621 ggcctcgaca agaagtcgat cgtcgacatc gtgaccgctc cgaacatcga ccaggatctc
     4681 gtccggcagg tcatggtcaa gcacgattcg ggcgtggacc tgctcctggc gccgccatct
     4741 cccgagaccg ccgagctcgt cacccaggac cacatgccga tcatcctcga gcagctccgg
     4801 gggctgtacg actacgtcct gatcgacatc gacaagcgcc tcgacgacgt caacctcggg
     4861 atcctcgagg ccgccgacac gatgttcgtc gtgatgacgg ccgacctgtc gtgcctgaag
     4921 aacgtccgcc tcgtcctcga gacgatcggc catctcgggt actcggcgca gaaggtccag
     4981 ctggtcctca accgctcgaa cgcgttcacc ggcatcagcg tgaagaacgc cgagggcgcc
     5041 ctgaagcgga cgatcgatca ccaggtcgtc aacgagtacc gcggcgcgat cagcgccctg
     5101 aactcggggg cgccgttcat gttcacgaag gcggactcgc tcctcggccg gtcgctcctg
     5161 gagttcgccc gggtcgtcga caagacaccg atcgcggccg cgacgccggc gctcgcgcgg
     5221 ggctgagcgg cgggcgattc gtcacgggcc gggtcctccg cccgttcggt cacccggcgc
     5281 ccgaaccgag gccggaaccc cgtcacgcgc ggagggccgt acaggcgcga tgaggccacg
     5341 gtgggccgcc ccgagccgcg atgagccgcg ctgagccgcg atgaaagccg aggtgagcgg
     5401 cggcaacgtc gccggtgatc ggcaggtgat cgccgcccgc tagggtcggg ccgtgctggc
     5461 caccctgttg tcgtccaccc tcgtcggtct cgatggccgg atcatccggg tcgaggtcga
     5521 cgtcgcacct ggcctgcccg ggttcacgat cgtcggcctg gcagacgccg cgctccagga
     5581 ggctcgcgag cgggttcgcg gcgcgatccg caatgccggc ttcagccatc cgccgcgccg
     5641 gatcacggtc aacctggccc ccgcggacct gcgcaaggcc ggcgcgtcgc tggacctcgc
     5701 gatcgccatg gggatcctgc tgggctccga gcaggtggtc gcggccggcg gtcgcgtcgc
     5761 cctggtcggc gagctctcgc tcggcggcga ggtctcccgc gtgcccggca tcctgccgat
     5821 gaccgcggcg ctccgccgcc gcgggatcgg ccgggtcatc gtccccgccg actcggcggc
     5881 cgaggcaggg ctcgtcgcgg ggatcgacat cgtcggcgtc cggacgctgc gcgaggcagc
     5941 cgatgccctt cgtcgccgcg gacgccgcac gacccgactc gctccacggc gcgtcgagct
     6001 ggccgagccc gggagcggcg agcccgggag cgatgagccc gagagcggcg agccgcttcg
     6061 gtccgggatc ggcgcggagg tcgcgtcgcc cgtcgacgcg acgccggacc tcgccgaggt
     6121 ccgcggtcag ctcgaggcgc gtcgggcgct cgaaatcgcg ctcgcgggtg gccacggcct
     6181 gctcctcgtc gggccgcccg gaaccggcaa gacgctcctt gcccgcacga tccgcgggtt
     6241 gttgccgcct ctcgacgatg ccgaggcgct cgcggcgacg atcgtggcgt ccgtcgccgg
     6301 cgaggggccg atccacgaac tgcgtcggag cgcgccggtc cgggcgccgc accacaccct
     6361 ctcgtatgcc gccatggttg gcggcgggcc gcggatgtcg ccgggcgaag tgaccctcgc
     6421 cgatcacggc gccctgttcc tcgacgagct gccggagttc ggtcgcgacg tgctcgaggc
     6481 gttgcgccag ccgctcgagg acggtttcgt gtcgatcgcg cgcgtcgggc gggcgacacg
     6541 ctttcccgcc cggttcctgc tcgtcgccgc catgaacccg tgcccgtgcg ggtatgccgg
     6601 cgatccgtcc gggcgatgcc gctgcccgat cggcacgccg gagcgttatg ccgggcggat
     6661 cagcggtccc cttcgcgacc ggatcgacct gtgggtgacg atggcccggg ttccaccgga
     6721 ggcgctgatc gccgggcgcg accccgagtc gtcggcagtc gttgccgcca ggatcgccgt
     6781 cgcccgcggc gtccaggcac gtcggacgat gcggctcaat ggacgactgg ccgggcgggc
     6841 gcttcgggcg gcgtgcgacc tgtcaccggc ggcgacggcc cgggcggtcg ccctcgccga
     6901 cctggagtcg ctctcgggac ggggcaccga gcggatcctc cgggtcgccc ggacgatcgc
     6961 ggacctcgcg gggtccgcgc gggtcgagcc cgaccacctc gacgaggcgg cgaggttccg
     7021 ttcgccggcg tcgcggctcg acgcccgcga agcgagctga tcatggtcga cggcgtggac
     7081 cggccgcagc tccggctcgt gacgtcgggc gacccgaagg cgtcgcccgg agcccgggga
     7141 ggagcaggtg gatcgaccgc cgtgccggac gagcgcgagg cctgggcggt cctgctgtcc
     7201 gtccacggcc tgggcccagc ggggttcggc gcgctcctgg ccgcttacgg cagcggtcgg
     7261 gcgatcctcg cggcggcgag cctgcgggga gcggcgccgc gcttcgcccg gatcgcggcc
     7321 gacggcgatg gacggccggc gttccgggaa ccggtcggag atgggatcgt cgcggcggcc
     7381 gccgcgcttg acgatcggct cgcggtgctg cgggccagcc tgctccggat cgtcaccctc
     7441 gacgatgccg cctatccgcc ccgcctccgg gcgatcgacc tgcctccgcc cgtcctgctg
     7501 gtccgcggcg aaccggcgac gctctcgtcc gcgcatgcga tcgccatcgt cggcaccaga
     7561 cgaccgaccg agcgcggtcg cctcatcgcc gcgcgcattg cgggcgcgct ggcaaaggcc
     7621 ggtgccgttg tcgtgtccgg actcgccgtc gggatcgacg gggccgctca cgccgcggtc
     7681 gaagcggagg gacggccgac cgtggcggtc ctcgggtccg gtcacggtcg gctgtacccg
     7741 cgggcccacc agcgccttgc caccgggatc gtcggggcag gtggcgcggt cgtgtccgag
     7801 ctgtggcccg atcagccgcc gtccgcccac acctttcccc agcgcaaccg ggtgatcagc
     7861 ggcctcgccg acgcgacgat cgtggtcgag gcgggcctga agagcggcgc cctgatcacg
     7921 gccaagtggg cgctggagca gggtcgcgac ctgttcctcg tgccgggccc gatcgacgag
     7981 acgcgatcgg ccggatgcct ccattggctt cgcgagttcc cgggcgaggc ccggatcgtg
     8041 gccacggtcc cggagctcat cgccgacctc ggcctgctcg accaggcaga cgaacccgcg
     8101 agcgtcggcc acggccccgg cgacgatggc accggcccgt ccgacccgcc acaccggccg
     8161 ccacggcccg ggctggacgc cgtcctggtc gagctcgggc cgaccgcgcg ggacgtcgga
     8221 gccgccctcg tcgcgggcca cggcagtctc gatgagctcg tggcggtcac cgagctggag
     8281 cctgcgaccg tgctcggagc gatcaccctc ctggagctgc gcggtctcgc gacgacgacg
     8341 tacggccgct atcgggcggc cggacagctc gcaagcgcca ccgccccgga ggtgaggtcc
     8401 gggtcgagcg gcaggttgcc cgttcggcgg ggcccgtgct agggtggcct tggccacggg
     8461 tggagtgagc gtggccgaca gccccggacg gtgtctcgtc ccccagcgaa ccgcgcagga
     8521 gactgtcgta ttgcgaaaac ttgccgccgc ggctctggcc gtccccgtcc tggccatcgt
     8581 gtacctgccg ttgctcgccc gccgctccgt ggcggcccgg gtcgggctcg ttgccagtgt
     8641 cggagtcatc gtcctcgtcg ccgcgctcgg cctgtcccgg cccgtcgtga cgaccgcgag
     8701 ccagccggcg cccccgatca ccgccctcgc cgatagcgcc ttccgctcga tcgatgcggc
     8761 gaccgacctc cacgctgcgg tcgacatcac cttcagcgag gcgatggacc cgcgcagcgt
     8821 cgcggcgtcg ctgactgtca cgccggcgac cgcgaccagc atcagctgga acgcggcgcg
     8881 aacggtcctg acggtccggc cgaccagcag ctgggcgcct ggcacgtacc acacgatcac
     8941 ggtcgcggcc ggcaccctcg cggccggcgg tcgtccgatg tcgaccgcgg tccgcgcggc
     9001 gttcattacc cgggcgccga cgagcggccg catcgcgccc acgctccggg cgggcaacgc
     9061 gacctcactc gcgacggcgt tccggatcac cttcgatcac ccggtcgcga tcgacgaggt
     9121 cgcgaccgcg ctcaagatca gtccgaccgt cgacggcaag ctcgtcgccg actccggccg
     9181 gcccgacgtc atcgcgacga gcgcgctcgg cttcacgttc actccggcgg cggtcctggc
     9241 cggcggaacc gcgtaccggc tgaccctcgg cccgctggtc gataccgacg gtgcggcggt
     9301 cggcagcgtc gagacggtcg cggtccggac gtcggctgca ccgcggatcg tccggttccg
     9361 gccggccgat ggcacgaaga aggtcgagcg ccgcgccgca ttgtcggtcc ggttctcgga
     9421 cccgatggac cacgtttcga cccgggccgc cttcacggtg accgccaacg gcaagccggt
     9481 gcacggtacg taccgtttcg ccgaatccag cacggtcctg gtcttccagc cggtctcggc
     9541 cctgccggcc ggggcgaagg tcgtgatcag cattggcgcg accgccacgt cggctcaggg
     9601 cgtgcagctg gcgcgggcgt cctccgccac cctcaccacg gtccccaagc gcaaggcggc
     9661 tccccgaacg gccccgaccg tcagccgcag ctcgtccggc agcggctcgt ccggcagcag
     9721 ctcgtccggc ggcggcgccg tcggcggcgg ctcgtggggc gcggtcgaga cctactacct
     9781 gcgactgatg aactgcaccc ggaccggcgg ctgggtcacc tcgaccggca gctgcagcag
     9841 ccccggcggt cggaacgtcg ccccgctcaa gctcgacgcc ggcatctcgt cgcacgtctc
     9901 gcgtccgtac gccaagaagc tcgccgtggg cggctggtgc gaccacttca tcggcggcaa
     9961 ccccggcgat cggctgcggt cggccggcta cacgagctac atctgggccg agaacctcgg
    10021 ctgccgctcg ggcaacccgt actcggcggt cctcggctcg cacctcttct tccagagcga
    10081 gaagccgtac agcggtggcc actacgtcaa cctgatgaac agcgcctacg accgggtcgg
    10141 catcggcgtc tgggtgtcgg gagggcgggt ccgcctggtg atcgacttct accacccgcg
    10201 ctaggaacac ccggcgggcc tgcgcacccc cgacccaacc cagccgtccg gcgacgaccg
    10261 ggaccatcgg gccgcagacg gggcccagcg ggcagcggac gatcggtccg tgatccgcaa
    10321 cgtcgtcatc cacatcagca acgagcagcc gctgctcgcc gacctctacg agatgccgac
    10381 cgcgctcgac gtcagcctcg tctgcacgaa catgcggatg ctcgacggca agaagccgat
    10441 cttcatcgac cattcgaact cggtcttcgt ctttccctac ctcaacatcc ggttcatcga
    10501 gatccttgtc ggggaagcga cgggcatgcc cgacctgccg ctcgacggtc gtgacgtcgc
    10561 cgccggcgaa acgaccgatg acaccgagag cgaagccgat ctcgagctcg acgaggactt
    10621 cctccgccgg atccgcgacg tctgaggatc gccggccggc gaccgacggc gagccccgtt
    10681 cagggtgatc atcgagttgg aacgccgacg agcccgcgct acgctccagc ggtgatccgc
    10741 ctcgccatcg tcgacgacca ccccgcgatc gccgtcgcgc tcgcggccgc gatccgcgac
    10801 cgcgccgacc tcgagctcgt cggctcggcg accgccactg ccgacgcgct cgcgctggtc
    10861 gagcggacgg cgcccgacgt cgtcctgtgc gatctctggc tcgacggcga gcccggcggg
    10921 ctcgacgtgc tcgcgtccct caccgcgtcc gaccggcgcg atgggccgcg tgtcctcgtc
    10981 ctgagcggct tcgatcagcc gagcttcctc cgggcggcct tcgagggcgg ggcggcaggt
    11041 tatgtgtcga agtccagccc ggtcgagtcg atcgtcgacg ccgtcctggc cgtcgcccgg
    11101 ggcgagacct gcttcccgcc ggtgaccctg cgggcgctcc gcgacgcacc gcgccggccg
    11161 agcgttcgcg agatgagtgc gatccggctc ctcgcccgcg gcgcctcgaa cgacgagatc
    11221 gcggtcgggc tcgggatcag catcaagacg gtcgagagcc atcttcgccg gctgttcggg
    11281 cggtacggcg tgctgagccg gaccgagctg gcgatgctcg ccgtccgcga gggatggctc
    11341 ggcatcggcc catgaggctc gccgtcgagc agcgtggtgt gcgctggtcg tggtcggctc
    11401 tcgacctcgc catcgccggg ttctcactca ccgcggtcgc gacgaccgta ctcagcctcg
    11461 gtcagccggc gatcaccccc atcggtccga tcccgatcct gctcgccctc ggcgcgttct
    11521 gcgtcgccgc cctggcccgc cggccggcac cgagcctcgc ctggatcgcc acgatcggcg
    11581 gctcctttgc ggcatcgtcc atcccgatcg accaggcccg ggcggcggat ccgggtcagc
    11641 tcggcgtggg accgtggctc gggctcgcgg tcccggcgag cgtcggtgcc atcgtcaccc
    11701 tcgggatcgc cgcccgctac gccacccggg ccgatcgacg cctgggccgg atcgcggtcc
    11761 ccgtgagcgt cgggttgttc gcctggctgg tcgtcgcatg cgccacgacg atcgggctga
    11821 tcggcacggg ccaggcgagg gcggatccgg agttcacctg gatcgatgtc gcgaccgcgc
    11881 cgatcgccgg attcgtgcgg ttcgtcgtcg tcgtcacgac gctcggtgtc ctggccgatg
    11941 tgcgcgccgg gtatgagcgg gcgcgagagc gtctggccgg tcccccgacg accacggccg
    12001 ccgccgagcg cgcctgggcc ctggccgtgg ccacgatccg tgagctcgtg cccgggcagt
    12061 cggcggccga ggaggcgagc cttgccgccg agcggacgcg tctcgccggc gacctccatg
    12121 cgacggtcct gccgggtctc cggcgggcga tcgcggaggc cgagtcgggc ggcgatccgg
    12181 acgtgctcgc ccgccagctg cggacggtcg atctcgagct cgagcggctg atggccgacc
    12241 gttggccggt cgtcctcgag gccttcggcc tggtggcggc gctcgaagat ctcgccgaac
    12301 ggatcgaggc cgacacgtca ctcggcgtcc agatcgatgt cgagcgggcc ggcgatcggc
    12361 cgccaccgac gatcgagcgg gcagcgtggc gggtcgccca gctcgcggtc gacaacgccg
    12421 cgcggcacgc cgaggccagc ctggtgacgg tgacggtgtc ggtcgacgtg gagcgcctcg
    12481 tgctcgcgat cgccgacgac ggacgcgggt tcgacccgga caccgccggg gctgtccggg
    12541 ccggggcgcg tggcctggcc gacgcgacgc gccgggcggc tgccgtcggg gcggcgatca
    12601 ggatcgagcc gcggcccggt ggcgggacga cggtgacgtt cgactggacc gcacatcgag
    12661 cgtgaggacg gccgcgtcgg atcgggcgcg ttgcgggtcg tcatcgcgtt gtcgtgccgg
    12721 gttcccgacg attggccgtg gcctcgccga gcgggaggat cgcggcgagt ccgccgaccc
    12781 tcggcgggac gaacgaccgg gaggccgccc atgacgctcc ttcgccgcat ccgcgctctg
    12841 cgcttcgtcg aaccggaccg cgaccgatcc acggatcgag ccggccaggg tctcgccgaa
    12901 tacgccctca tcctcgccct gatcgcgatc gtcgcgatca ttgccctcat cttcctcggc
    12961 ggtcagatca gctcgatcct cagccacgtc gggacatcga tctgacgact gccgcgagcc
    13021 gccggcccgg gcctcgaggg gagcccgggg cggccacctg ccatccgaca gcgcccggcg
    13081 ctccgcgccg ggtcccggcc cgcccgatca ccgggcgccc tcatcgaggg cgaaccgggc
    13141 gcggcgttcc cggagctctg ctcctccttc tggtgggact cgcgaccggg ggctgcaacg
    13201 tcatcgccgg gccgccggag atcctcgacg gtctgagcat cggcgccgac gcgggctgcg
    13261 gagcgtgtga cgcgcccgac acctccgcca actgcggggc ctgcgaaagc atcgccacgc
    13321 tcgccgtccg gcatgtcgac gagacctggc cggatcatcc cgagatcggg acgatgtcgt
    13381 tccaccgcga gagctggtac ccgggcgaga acggcgagag gatcctccac acccgctccg
    13441 gatcgctggt cgtcgcggtc gtgaccttcg tcgacggcac acgccatgcc gcgtcggtct
    13501 actgtggcgt cggcggctgc cggtagggat cgggcgctcg tcgggcttcg cccgacgccg
    13561 gcgcgcggcc ggatcgcgcg aatgcccagg tggctggcgg tgcaggaccc tgtgctacgc
    13621 tcccggccgt gtcaaagaac ctcgtgatcg tcgaatcgcc tgccaaggcc cggacgatcg
    13681 aacgctacct gggtgccgac tacaaggtcc tcgcgtccta cggccatgtc cgcgatctgc
    13741 ccgagaaccc cggcaagggc aagttcgggg tcgatgtcga ccatgacttc gccccggaat
    13801 acgtggtctc cgacgaccgc cgcaagcagg tcgcggagat cacgaaggcg gccaagtcgg
    13861 ccgagcacgt cttccttgcg accgacctcg atcgggaagg ggaggcgatc gcctggcacg
    13921 tcgccgaggc cgctcatgtc ccggccagca agacccagcg ggtgacattc agcgagatca
    13981 ccgagggcgc gatccgcgag gcgttcgccc acccccgggg catcgacacg aacctcgtcg
    14041 acgctcagca gacgcgccgg atcgtcgatc gcctcgtcgg ctacaccctg agcccgctcc
    14101 tgagccggaa ggtccgctcg ggcctgtcgg ccggtcgggt ccagtcggtg gcggtccgcc
    14161 tggtcgtcga gcgcgagcgc gagatcacgg ccttcacggc ccgcgagtat tggaccctgg
    14221 aggtcctcct ggccacggtc gacggcgcgg tgttcgccgc cgagttggtc cggatcgacg
    14281 gcgccgcgct cgacgtgggc gacgaggaga ccgccgcgcg gcatgccgcc gctctgcgcg
    14341 acctcaagcc gcgggtcacc aaggtcggaa cccgcaagca gagccgcagc cccgccccgc
    14401 cgttcacgac ctcgaccctc cagcaggagg ccagccgcaa gctcggcttc agccccaagc
    14461 ggacgatgtc ggtcgcgcag cgcctgtatg aaggtgccga taccccggac gggcatgtcg
    14521 ggctcatcac ctacatgcga accgattcga ccgcgatcgc cggcgtggcg atgggcgagg
    14581 cccgcgacgt catccgggcc cggttcggcg acccgtacac gatgccgaag gggcgggtct
    14641 acaggaccaa gtcgaagggc gcccaggagg cccacgagtc gatccgcccg acctcgttcc
    14701 ggcgcgaccc ggattcgctc gccggcaccc tcgccaacga cgagctccgc ttgtaccgcc
    14761 tgatctggca gcgggcgatc gcctcgcaga tggcggccaa ggagctcgag acgaccacgg
    14821 tcgatctcgt ggccgggcca tacgagctcc gggcgagcgc gacgaagacg ctgttcgacg
    14881 gcttctccgc ggtctacacg gagggacggg acgacggcgc ggccgaggac gtggaggcgg
    14941 aacgcgcgct gccgccggtc aacgagggcg acctcaccac ggtccgcgag gtcacgcccg
    15001 cccagcactt caccgagccg ccgccgcgct tcacggaggc gacgctgatc aaggccctcg
    15061 aggagcacgg gatcggccgg ccatcgacct atgcggcgac gatctcgacc atcctcgatc
    15121 gcggctatgt ccgggtcgag gagcggcgtc tccatccgga gctgatcggg aacatcgtca
    15181 ccgactggct ggtcgagcat ttcggcgagt acgtcgatgt cgcgttcacc gcccggatgg
    15241 aggaggagct cgacgaggtc gcccgcggcg agcgcccgtg ggtcccgttc ctgcgcgcgt
    15301 tctacgaccc gctcgccgag ctcgtggccg agaagcggaa gacgacgacc cggccgaccg
    15361 agcccaccga cgaggtctgc tccgaaggcc acccgatggt catccggctc gggcgaaacg
    15421 ggcggttcct cgcctgctcg ctcttcccgg agcacaagga atcgcgcccg ttgccgggcg
    15481 aggaaccccc gccccaggcc ggcgagggcg aggtctgccc ggaatgcggc gaggggacgc
    15541 tggtcggtaa gcgcggccgg ttcgggccgt tcgtcggctg ctcgcgctac cccgactgca
    15601 cgttcatcaa gaaggagggc ccgccgccac cggacccgct cccgttcaag gtaacctgcc
    15661 cgaagaacaa cgacggccag ctgatcccgc gtcgcgcccg ccggaccggg aacgtgttct
    15721 acggctgctc gaactacccc aagtgcgact acacgacgaa cttcgagcca gtcggcgcgc
    15781 tccacgacac tgacgacggc ccggtcgcga agcacggcga cgccgggatc tgcctgcgct
    15841 gcggcgccgc gatcccgctc gccgagggcg tcgacgtcgc cggggtcaag ctcgagggcg
    15901 gaccgccgaa ccccgaggcc ctcgccaagc cggc
//
LOCUS       DATAIW010000024         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_30684, whole genome shotgun sequence.
ACCESSION   DATAIW010000024 DATAIW010000000
VERSION     DATAIW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30684"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..1103
                     /locus_tag="VHM48_00930"
     CDS             477..1103
                     /locus_tag="VHM48_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753993.1"
                     /translation="MPDRPLDLTRLHAATAILAARDPDLGGILARLGPPPLWDRPPGL
                     ATLVAIVLEQQVSLRSGAAALERLRLAAGSLEPAAIVALGEEGARAAGLTRQKARYVV
                     GLAAATLDRRFDPATVAVTDDDAARSSLMALVGVGRWTADIYLLMALGRPDVWPDGDL
                     ALAAAMRRAKGLTMVPDAASQRRVAEAWRPWRAVGARLLWHAYLAGER"
     gene            1296..2465
                     /locus_tag="VHM48_00935"
     CDS             1296..2465
                     /locus_tag="VHM48_00935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX2753994.1"
                     /translation="MRLKLFAIVLLLVVGGAAVFVALGGLPRSSAASTTYLTGTATVA
                     DVSDDVAATGSIASSTTWSLAFGSAPTTTDTAAAATQDGTWIVRAVTAHVGDIVTKGQ
                     VLATAANPTLTANIIAAKNDWTGAALQRTSAQEAYDAATTTVAIRQARGTLLNAKNAI
                     AAAHTKLIDLKKTATLNRLVAPAAGIVTAVNITPGADAPSGAAITIASTDYQVTAEVV
                     ESDVSSMKLEQPATVTVAAIGADLAGTVTAIAPIASTGSSGGVVSFAVTVALTNPPAT
                     LRSGMTADVTITTASATGVLAVPAAALRGLAGNYSVLVLTAAGSPEAKSVTVGLMTSS
                     LVEIQSGLNAGDVVVTGTSSTQRTTTGTGIGGGGAGTFVVPGGGAGGLGGRGNNP"
     gene            2467..3141
                     /locus_tag="VHM48_00940"
     CDS             2467..3141
                     /locus_tag="VHM48_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2753995.1"
                     /translation="MQPSIVSLERISRTYAMERVRVEALRDVSLEVAQGEFVAIVGPS
                     GSGKSTMMHIIGCLDRPTAGIYRLAGTPVASLGDDGLARLRSRTIGFVFQSYNLLPRT
                     SALDNVATPLLYQGVGRRERVARATAALERLGLGDRLDHEPTELSGGQQQRVAVARAI
                     VTDPALILADEPTGNLDSASGRDVLAVLRELHASGRTIVLITHDAEVAAAAERQVHIR
                     DGMIAA"
     gene            3138..>3695
                     /locus_tag="VHM48_00945"
     CDS             3138..>3695
                     /locus_tag="VHM48_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2753996.1"
                     /translation="MNALELLRLALSRLRTSRLRAALTMLGVVIGVASVVALVGVGQG
                     TTANITSRLAGLGTNLLTISPTGRSTTSGLTLEDAAAIGRVEGVAAVAPEIQTSASIV
                     VGTKSTTTSVVGTSAQYARVRAYDVWQGSFLTDATVESGLRVAVLGATTASDLGLDAT
                     SLGSTISVDGLPFRLIGILQPKGGSG"
BASE COUNT          481 a         1378 c         1328 g          508 t
ORIGIN      
        1 aggaccgcct cggcggtgcg cctggcgtcg atggcgtcgt cgaggcggtc gttggccggc
       61 ggctgctcac ggccgccggt ggcggcaggg tcacgagtcg gcggcgcgtc acgaaacggt
      121 tcgctcacgg gtggtctccg cacccgtggg cacgggcgag atgcttctgg tcgggcggtc
      181 acctcccgcg gttggaccgg accgttggcc gaaggctcgg tggaacggat gagaggggca
      241 tgaaagccgc cgggcgcgct gccgggcgcc cgccgccgcg gggtcgtgtt ccagcgcgcc
      301 gcccgaatcg gggcgccctc ccgtttcgaa tagccgcata tcccctgtcc ggtattcggc
      361 tattcgcatg tgcggcgcgg ggacagttga cacgggcgac cgagcgggag cgcgggcacg
      421 gcgacatcga cgtcggctgc aaccggacgg cagcggaccg ggttaccgtc gtcaccatgc
      481 ccgaccggcc cctcgacctc acccggctgc acgccgcgac cgcgatcctc gccgcccgcg
      541 accccgacct cggaggcatc ctggcccgcc tcggcccgcc gccgctgtgg gaccgcccgc
      601 ccgggttggc gacgctggtg gcgatcgtcc tcgagcagca ggtgtcgctg cgctccggag
      661 cggcggcgct cgagcgtctc cggctcgcgg caggctcgct cgagcccgcc gcgatcgtcg
      721 ccctgggtga ggagggcgcc cgggcggccg gcctcacgcg tcagaaggca cgctacgtgg
      781 tcggcctcgc ggcggcgacc ctcgaccggc ggttcgatcc ggcaacggtg gcggtgaccg
      841 acgacgacgc cgcccgctca tcgctcatgg cgctcgtggg tgtcggacgc tggaccgccg
      901 acatctacct gttgatggcc ctcggccgcc cggacgtgtg gcccgatggc gacctcgccc
      961 tggcggccgc gatgcgtcgc gccaagggtc tcacgatggt gcccgacgca gcgagccagc
     1021 gccgcgtggc ggaggcctgg cggccgtggc gcgcggtcgg ggcacggctc ctctggcatg
     1081 cctacctcgc cggcgagcgc tgacggaacg gccggcggtc gaccggtccg gcgacgccgg
     1141 gtatgctcgc gctcgaccga cggcggccgg gcgggcatcg gaggggggcg ctcgagccgc
     1201 tcgccgtccc ggctctcatc gccttctcac ctgatccgtc gacatttcgc ctgtcgggcg
     1261 ccgcgattcg gtgcgccccg ttgcgtgggc gtcacatgag attgaagctg tttgccatcg
     1321 tcctcctcct ggtcgtcgga ggagcagcgg tcttcgtcgc cctcggcggg ctgccgcgca
     1381 gctccgccgc gtcgaccacc tacctcaccg ggaccgcgac cgtcgccgat gtcagtgacg
     1441 acgtcgccgc gaccggctcg atcgcgtcgt cgacgacgtg gagcctcgcc ttcggatcgg
     1501 ctccgaccac gacggacacc gcggccgcgg ccacccagga cgggacctgg atcgtgcggg
     1561 ccgtgaccgc ccatgtcggt gacatcgtca cgaagggcca ggtcctcgcg accgcggcga
     1621 acccgacgct gaccgcgaac atcatcgcgg cgaagaacga ctggacgggc gccgcactcc
     1681 agcgcacctc ggcccaggag gcctacgacg cggccacgac caccgtcgcg atccgccagg
     1741 cccggggcac cctgctgaac gcgaagaacg ccatcgcggc cgcgcacacg aagctgatcg
     1801 acctcaagaa gaccgccacc ctcaaccgac tcgtcgctcc ggcggccggg atcgtcaccg
     1861 ccgtcaacat cacgcccggc gccgacgcac cgtcgggcgc ggcgatcacg atcgcctcga
     1921 ccgactacca ggtcaccgcc gaggtcgtcg agagcgacgt cagctcgatg aagctcgagc
     1981 agcctgcgac cgtcaccgtg gcggcgatcg gtgccgatct ggccgggacg gtgaccgcca
     2041 tcgccccgat cgcgtcgacc ggctcgtccg gcggcgtcgt ctcgttcgcg gtgaccgtcg
     2101 cgctcaccaa cccaccggcc acgctccggt ccgggatgac ggccgacgtg acgatcacga
     2161 cggcatcggc gacgggcgtg ctcgcagtcc cggcggcggc cctgcgcggt cttgccggca
     2221 actactcggt cctcgtgctc acggccgccg gatcgcccga ggcgaagtcg gtgaccgtcg
     2281 ggctgatgac gagctcgctc gtcgagatcc agtcgggcct gaacgccggc gatgtcgtcg
     2341 tcaccgggac cagcagcacc cagcggacga cgaccgggac gggcatcggt ggcggcggag
     2401 ccgggacgtt cgtcgtcccc ggcggcggag ccggcggcct cggcggccgc ggcaacaacc
     2461 cctgacatgc agccgtcgat cgtcagcctc gagcggatca gccggaccta cgcgatggag
     2521 cgggtccggg tcgaggccct ccgcgacgtc tcgctcgagg tcgcccaggg cgagttcgtc
     2581 gcgatcgtcg gcccgtccgg ctccggcaag agcacgatga tgcacatcat cggctgtctc
     2641 gaccggccaa cggccgggat ctaccgcctg gccgggacgc cggtcgcctc gctcggcgac
     2701 gacggtctcg cccggttgcg gagccggacg atcgggttcg tgttccagtc gtacaacctg
     2761 ctgccgcgga cgtccgccct cgacaacgtc gccacgcccc tcctctacca gggtgtcggg
     2821 cgccgcgagc gcgtggcccg ggcgacggcc gcgctcgagc ggctcggcct gggcgaccgg
     2881 ctcgatcacg agccgaccga gctgtcgggc ggccagcagc aacgggtcgc ggtcgcccgg
     2941 gcgatcgtca ccgaccctgc cctcatcctc gccgacgagc cgaccggcaa cctcgacagc
     3001 gcttcggggc gcgacgtgct ggcggtcctg cgggagctcc acgcgagcgg ccggacgatc
     3061 gtcctcatca cccatgacgc ggaggtcgcc gctgccgccg agcgccaggt ccacatccgc
     3121 gacgggatga tcgcggcgtg aacgcgttgg agctgctccg gctcgcgctg tcgcgcctcc
     3181 gcacgagccg gctgcgggcc gccctgacga tgctgggcgt cgtcatcggg gtcgcttcgg
     3241 tcgtggccct cgtcggggtc ggccagggga cgaccgccaa catcacctcg cgcctggcgg
     3301 gtctcgggac gaacctgctg acgatcagcc cgaccgggcg gtcgacgacg tccggcctca
     3361 ccctcgagga cgcggccgcg atcggccggg tcgagggcgt cgcggcggtg gccccggaga
     3421 tccagacctc cgcttcgatc gtggtcggca cgaagtcgac gacgacctcg gtcgtgggga
     3481 ccagcgccca gtacgcccgg gtccgcgcct acgacgtgtg gcagggctcg ttcctgaccg
     3541 atgccaccgt cgagagcggg ctgcgggtgg cggtcctcgg cgcgaccacg gcatcggacc
     3601 tcggcctcga cgcgacctcg ctcggctcaa cgatctcggt cgacgggctg cccttccggc
     3661 tgatcgggat cctccagccg aagggcggct ccggg
//
LOCUS       DATAIW010000025         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_788, whole genome shotgun sequence.
ACCESSION   DATAIW010000025 DATAIW010000000
VERSION     DATAIW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..>1799
                     /gene="groL"
                     /locus_tag="VHM48_00950"
     CDS             268..>1799
                     /gene="groL"
                     /locus_tag="VHM48_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX2753997.1"
                     /translation="MNILADAVKVTLGPRGRNVVLEKKWGAPTITNDGVSIAKEIELE
                     DPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPLGLKRGIE
                     KAVEAVASQLLADAKEVETKEQIAATASISAADPAIGELIAEAMDKVGKEGVITVEES
                     NTFGLELELTEGMRFDKGYISLYFATDAERQEAVLEDPYILLFGSKISAVKDLLPLLE
                     KIIQSGKSLLIIAEDVEGEALATLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDIAILT
                     GGQVITEDVGLKLENTDIALLGTARKVVVTKDETTIVDGGGDAEQIAGRVSQIRSEIE
                     KSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNAKAAVEEGIV
                     AGGGVGLLQAAIKAFEKLELTGDEATGANIVRVAVEAPLKQIAINAGLEGGVVAEKVK
                     GLPAGEGLDAATGEYKDLIKAGIIDPAKVTRSALQNAASIAALFLTTEAVVADKPEKA
                     SAG"
BASE COUNT          337 a          583 c          621 g          258 t
ORIGIN      
        1 tgggagtgct aaacctggtg actggcactc ggtcatgccg agtgccaggt cggtgcggtg
       61 agaattcgcc ggtccgggac caccgatggt agccgggccc ggggggttcg tccgtcgcgg
      121 gcatcgccgc cgtccagcac accgtcaagc tgctgaccgc gtcctcgggc catcgcgccc
      181 aagggaccgg cgaatctgga ggaccaacac ctcatgtcca agctgatcgc tttcgacgag
      241 gaagcgcgtc gcgggctcga gcgcgggatg aacatcctcg ccgacgccgt caaggtgacg
      301 ctggggccgc gtggccgcaa tgtcgtcctt gagaagaagt ggggcgcgcc gaccatcacc
      361 aacgatggcg tgtcgattgc caaggagatc gagctcgagg acccgtacga gaagatcggg
      421 gccgagctcg tcaaggaagt tgccaagaag accgacgatg tcgccggtga cggcaccacc
      481 actgccaccg tgctcgccca ggccctggta cgcgagggtc tgcgcaacgt ggccgccggg
      541 gccaacccgc tcggcctgaa gcgcggaatt gaaaaggcag tggaagccgt cgcttcccag
      601 ctgctggccg acgccaagga ggtcgagacc aaggagcaga tcgctgccac cgcctcgatt
      661 tccgccgctg acccggccat cggcgagctc atcgccgagg cgatggacaa ggtcggcaag
      721 gaaggcgtca tcaccgtcga ggagtcgaac accttcggcc tcgagctcga gctcaccgag
      781 ggcatgcgct tcgacaaggg ctacatctcg ctgtacttcg ccaccgacgc cgagcgtcag
      841 gaggccgttc tggaggatcc ctacatcctg ctgttcggct cgaagatctc ggccgtcaag
      901 gacctactgc cgctgctgga gaagatcatc cagtccggca agtcgctgct gatcatcgcc
      961 gaggacgtcg agggcgaagc ccttgccacc ctggtggtca acaagatccg cggcaccttc
     1021 aagtccgtgg ccgtcaaggc ccctggcttc ggtgaccgcc gcaaggccat gctgcaggac
     1081 atcgcgatcc tgaccggtgg ccaggtcatc accgaggacg tcggcctcaa gctggagaac
     1141 accgacatcg cgctgctggg caccgcccgc aaggtcgtcg tgaccaagga cgagaccacc
     1201 atcgtcgacg gtggcggcga cgccgagcag atcgccggcc gggtctccca gatccgttcc
     1261 gagatcgaga agtcggactc ggactacgac cgcgagaagc tgcaggagcg gctggccaag
     1321 ctggccggcg gcgttgcggt gatcaaggcc ggagccgcga ccgaggtcga gctcaaggag
     1381 cgcaagcacc gcatcgagga tgccgtccgc aacgcgaagg ctgcggtcga ggagggcatc
     1441 gttgccggcg gtggcgtcgg gctgctgcag gccgccatca aggcgttcga gaagctcgag
     1501 ctgaccggtg acgaggccac cggcgcgaac atcgtccggg tcgccgtcga ggccccgctc
     1561 aagcagatcg cgatcaacgc cggtctcgag ggtggcgtcg tggcggagaa ggtcaagggt
     1621 ctgcccgcgg gcgagggcct tgacgccgcc accggcgagt acaaggacct gatcaaggcc
     1681 gggatcatcg atccggccaa ggtcacccgg tccgcgctgc agaacgccgc gtccatcgcc
     1741 gcgctgttcc tgaccacgga ggccgtggtt gcggacaagc cggagaaggc gtcggccgg
//
LOCUS       DATAIW010000026         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_849, whole genome shotgun sequence.
ACCESSION   DATAIW010000026 DATAIW010000000
VERSION     DATAIW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1492)
                     /locus_tag="VHM48_00955"
     CDS             complement(203..1492)
                     /locus_tag="VHM48_00955"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX2753998.1"
                     /translation="MKKVVLAYSGGLDTSVAVAWLREKYDCDVVCLTVDVGGGSLRDG
                     VEKRALSAGASRAYVVDARERFVRDFVWPHLQANALYQDAYPLATALARPLIAQLLVE
                     VARREDADAVAHGCTGKGNDQVRFDVAVHALDPGLEVIAPMRVGMGLTRDQEIDYAAE
                     RGIEIPITKASPYSIDVNLWGRSCETGVLEDPWVTPPPDAYEWTVAPSDAPAPVELVI
                     EFEGGIPVALDGERLDPVALVERLHELGGAHGVGRVDHVEDRLVGIKSREIYEAPAAT
                     ILHAAHGALEGLTLTKDTLRFNRLVASELAQLTYDGLWFSALHRDLRGYVASAQRVVS
                     GEVRVRLDHGSAVVVGRRSPMSLYDKNLATYDKGDAFDHAAAVGFISIFGLPLRVEAA
                     RHGAVGAHYGAAWTWTDPLLADLPTTVTDPSEAVAAG"
     gene            complement(1519..2073)
                     /locus_tag="VHM48_00960"
     CDS             complement(1519..2073)
                     /locus_tag="VHM48_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2753999.1"
                     /translation="MASRSPSAVAPTHAAGVGSKRDRQRAIRELVGAGAIGSQQELVD
                     RLIGRGFAVTQATVSRDIADLGLVKVSRADGHLYVMPESLAAGSSRPSAAGPTDERLR
                     RILSDIPVTVGRSGLILLVTGSPGTASVIAQAIDESTLTEQEGTLAGDNTLLVLFADE
                     ARLLAWRARFDHIVATALAPAARA"
     gene            complement(2112..2897)
                     /gene="argB"
                     /locus_tag="VHM48_00965"
     CDS             complement(2112..2897)
                     /gene="argB"
                     /locus_tag="VHM48_00965"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2754000.1"
                     /translation="MSEILVVKLGGTTIADQQHVLAEVAAVARKRPVVLVHGGGKRIT
                     EWLDRMGVPSRFENGLRVTDQAALEVAAAVLRGVVNSELVAGLRDLGVDAVGLSGVDG
                     GLLVSERVPGMGLVAHVVGLRRDLLDAVLVGGQVPVVAPLARDEQGVVCNVNADDAAA
                     GIAAGIGARQLVLMTDVDGVRDADGRRLDTLTSGEAESLIESGVIAGGMVPKIRAALA
                     AIAWEGSEAIIADASADGSLERALTDPTFGTRITAGRSAVGAA"
     gene            complement(2950..4347)
                     /gene="argJ"
                     /locus_tag="VHM48_00970"
     CDS             complement(2950..4347)
                     /gene="argJ"
                     /locus_tag="VHM48_00970"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX2754001.1"
                     /translation="MATATTTHLSALSTDLPPVERRAAIPGGFVATGGAAGIKASGRP
                     DLALVATDAGPDGRRVPAAAAAVFTPNAFAAAPVRLSQVHLAATDPSGNGRFGWATGI
                     ISTSGCANAATGAAGDADQAEIAAMLAATLGIPEDETLLLSTGIIGTRLPLDIVRAGV
                     AAVAPTLAPSDDGLAAAAEALRTTDSLVKAATATITLPDPDGQAVRSVRVTGIAKGVG
                     MIHPRMATMLSIVLTDATVDPATLHGLLRPVAARTWDQLSVDGDTSTNDTVFVLASGA
                     AGAAPVAAGTHVATELGRAIEAVARDLARQQAADGEGATTLITCQVSGAVDDADARAV
                     ARAVISSSLVKAAAHGRDPNWGRIAGAAGNATLADEAVLAAGGLTASAARSRAGTPAE
                     LDPDRLRIAIAGHLVFDGNVGGPVVFDRATARAAMDGPELLVRLDLGLGDGHGEAFGC
                     DLTETYVTENSEYTT"
     gene            complement(4362..>5356)
                     /gene="argC"
                     /locus_tag="VHM48_00975"
     CDS             complement(4362..>5356)
                     /gene="argC"
                     /locus_tag="VHM48_00975"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX2754002.1"
                     /translation="IRLLGRHPTVEIVGLKGRDRHEPVGVSHPHLSGTGLAVGSDLPE
                     VDAVFLALPHGTAAGLVPEIAAAGTAVIDLGPDFRLRAALDYPRWYGFEHPRADLLGS
                     AVYGLPELHRAELRALRHAPLAIVGSPGCYPTATLLALAPLARAGLIGDLVVDAKSGV
                     SGAGRDLKPDLLFGEVNESVKAYGVGGHRHVAEIEQELAGIGAEAGVTNPGIGAVDFL
                     PHLIPMTRGILSACHVRPTRPTSQAELDDLYASAYADEPFVTVVPVAPATKHVLGSNQ
                     VRVFVRLDERTGRILAIGVIDNLVKGAAGQAIQAFNVVHGLTETAGLEQLPLAP"
BASE COUNT          744 a         1896 c         2019 g          697 t
ORIGIN      
        1 accgaggggc catcagcgcg ccacggaagt cgcgtccacc gccggctggt ccggcgggcg
       61 gaccaccgga cgcctgagcg gcgtgagcga ggccaaaggt ggcccgccgg ccgtcgcttg
      121 gtccgtgccg cggaccacgc ccggcccaac ggcgccgggc acgcaacgaa gcccgcgtcg
      181 accaacgcga ccggtcggtt cctcagcccg ccgcgaccgc ctcggacgga tcggtgaccg
      241 tggtcggcag gtcggcgagg agcggatcgg tccacgtcca ggccgcgccg tagtgggcgc
      301 cgaccgcccc gtggcgggcg gcctcgaccc gcagcggcag gccgaagatc gagatgaacc
      361 cgacggcggc cgcgtggtcg aacgcgtcgc ccttgtcgta cgtggcgagg ttcttgtcgt
      421 acagcgacat cggtgagcgc cgcccgacca cgaccgcgga gccgtgatcg agccggaccc
      481 ggacctcgcc gctgacgacc cgctgggccg acgcgacgta gccccgcagg tcgcgatgca
      541 gcgcgctgaa ccacagcccg tcgtacgtca actgggccag ctcgctcgcg accaggcggt
      601 tgaagcggag ggtgtccttg gtgagcgtca gcccctcgag cgcgccgtgt gccgcgtgga
      661 ggatcgtcgc cgcaggcgcc tcgtagatct cgcggctctt gatcccgacg aggcggtcct
      721 cgacatggtc gacccggccg acgccgtggg cgccgcccag ctcgtggagt cgttcgacga
      781 gggcgaccgg gtcgagccgc tcgccgtcca gggcgaccgg gatgccaccc tcgaactcga
      841 tcaccagctc gaccggggcc ggggcgtccg acggggcgac cgtccactcg taggcgtccg
      901 gcggcggcgt gacccagggg tcctcgagca cgcccgtctc gcacgagcgg ccccacaggt
      961 tgacgtcgat cgagtagggc gaggccttcg tgatcgggat ctcgatcccg cgttcggcgg
     1021 cgtagtcgat ctcctggtcg cgggtcaggc ccatcccgac ccgcatcggg gcgatgacct
     1081 cgaggcccgg atcgagggcg tggaccgcga cgtcgaagcg gacctggtcg ttgcccttgc
     1141 cggtgcagcc gtgggcgacc gcgtcggcgt cctcgcgtcg cgcgacctcg acgagcagct
     1201 gcgcgatcag cggccgggcg agggccgtgg cgagcggata cgcatcctga tagagggcgt
     1261 tggcctggag gtggggccag acgaagtcgc ggacgaaccg ctcccgggcg tcgacgacat
     1321 aggcccgcga cgccccggcg ctgagcgctc gcttctcgac gccgtcgcgg agcgagccgc
     1381 cgccgacgtc gacggtcagg cagacgacgt cgcagtcgta cttctcgcgg agccaggcga
     1441 cggcaacgga cgtatcgagc ccgccggagt aggcgaggac gaccttcttc atgatcagcg
     1501 ggcctccggt gatgaacgtc aggcgcgggc cgccggggcc aacgcggtcg cgacgatgtg
     1561 gtcgaagcgc gcgcgccagg cgaggaggcg ggcttcgtcg gcgaacagga cgaggagcgt
     1621 attgtcgccc gcgagcgtgc cttcctgctc cgtgagggtc gattcgtcga tcgcctgggc
     1681 gatgacgctg gccgtccccg gcgagccggt cacgagcagg atcaggccgc tccgtccgac
     1741 cgtgacgggg atgtcggaga ggatccggcg gaggcgctcg tcggttggtc cggccgcact
     1801 cgggcgagac gatccggccg ccagcgactc gggcatgacg tacaggtggc cgtccgcgcg
     1861 cgacaccttc acgaggccga ggtcggcgat gtcgcgactg accgtggcct gggtgaccgc
     1921 gaagccgcgg ccgatcaagc ggtcgaccag ctcctgctgg ctgccgatcg cgcccgcacc
     1981 gacgagctcc cggatggcgc gctggcggtc gcgcttcgag ccgacgccgg cggcgtgggt
     2041 cggggcgacc gcggacgggc tccggctggc catgctcgct gtggcatcca ccggcgcggg
     2101 tcgaccgcgc atcaggccgc gcccacggcg gatcggccgg cggtgatccg ggtgccgaag
     2161 gtcgggtcgg tcagggctcg ttcgagcgac ccgtccgccg atgcgtcggc gatgatcgcc
     2221 tccgacccct cccaggcgat tgccgccagc gcggcccgga tcttcgggac catcccgccg
     2281 gcgatgacgc cgctctcgat gaggctctcc gcctcaccgc tcgtgagcgt gtcgagccgc
     2341 cggccgtcgg cgtcgcggac gccgtcgaca tcggtcatca ggacgagctg gcgggcgccg
     2401 atcccggccg cgataccggc cgcggcgtca tcggcattga cgttgcagac gacgccctgc
     2461 tcgtctcggg cgagcggcgc gacgaccggc acctggccac cgacgaggac ggcatcgagc
     2521 aggtcgcggc ggaggccgac gacgtgggcg acgagcccca tccccgggac gcgctcgctg
     2581 acgaggagac cgccatcgac cccggacagc ccgaccgcat cgacgcccag atcgcgcagc
     2641 ccggcgacga gctcgctgtt gacgacaccg cggagcacgg ccgcggcgac ctccagcgcg
     2701 gcttggtccg tgacccgcag gccgttctcg aaccgcgacg ggacgcccat ccggtcgagc
     2761 cactcggtga tccgcttgcc gccgccgtgg acgaggacaa ccggccgctt ccgggcgacc
     2821 gcggcgacct ccgccagcac gtgctgctga tccgcgatcg tcgtcccacc cagcttgacg
     2881 accagaatct cgctcatccg agttgcctcc ccggcccgcc ggcgcgccgc tggagggtcg
     2941 gccggtgatt catgtggtgt actcagagtt ctcggtgacg tacgtttcgg tgagatcgca
     3001 gccgaaggcc tcgccgtggc cgtcgcccag cccgaggtcg aggcggacga ggagctccgg
     3061 gccgtccatc gcggcgcgcg ccgtggcacg atcgaagacg accgggccgc cgacgttccc
     3121 gtcgaacacg aggtgtccgg cgatcgcgat ccggagccga tccgggtcga gctcggcggg
     3181 ggtgccggcc cggctcctgg cggccgatgc cgtcagccct ccggcggcca gcacggcttc
     3241 gtcggcgagg gtggcgttgc cggccgcgcc cgcgatccgg ccccagttcg ggtcgcgacc
     3301 gtgggcggcc gccttgacga gcgacgacga gatgacggcg cgggcgacgg cccgggcgtc
     3361 ggcatcgtcg accgcacccg acacctggca ggtgatcagc gtcgtcgctc cttcgccgtc
     3421 ggccgcctgc tgccgggcga ggtcgcgggc aaccgcctcg atggcccgcc cgagctcggt
     3481 cgccacgtgg gtcccggcag cgaccggcgc cgcgcccgca gcaccggaag ccaggacgaa
     3541 cacggtgtcg ttggtcgagg tgtcgccgtc gacgctgagc tggtcccacg tccgcgcggc
     3601 cacgggccgg agcaggccat ggagcgtggc gggatcgacc gtggcatcgg tcaggacgat
     3661 cgagagcatc gtcgccatgc gtggatggat catcccgacg cccttggcga tgccggtcac
     3721 ccgcaccgag cgaaccgcct gcccatccgg atcgggcagc gtgatcgtgg ctgtcgccgc
     3781 cttgacgagc gaatcggtgg tccggagcgc ctcggccgca gccgccaggc cgtcatcgct
     3841 cggtgccagc gtcggcgcca cggccgcaac gccggcccgg acgatgtcga gcggcaggcg
     3901 cgtcccgatg atcccggtcg agagcaggag cgtctcgtcc tcggggatgc cgagggtcgc
     3961 ggcgagcatc gctgcgatct cggcctggtc ggcgtcgccg gccgcgcccg tcgcagcgtt
     4021 cgcacagccg ctcgtcgaga tgatcccggt cgcccagccg aaccggccgt tgcccgatgg
     4081 atcggtcgcc gcgagatgga cctgcgagag acggaccggt gcggcggcaa acgcgttcgg
     4141 cgtgaagacc gcggccgcgg ccgccggaac ccgacggccg tcgggacccg catcggtcgc
     4201 gacgagcgcc aggtccggac ggccggaggc cttgatcccg gccgcgccgc cggtcgcgac
     4261 gaagccaccc gggatcgcgg cgcgccgctc gaccggcggg agatcggtcg agagcgcgga
     4321 caggtgcgtg gtggtggcgg tggccatcgg tccttcgggg gctagggggc gagcgggagc
     4381 tgctcgagac cggcggtctc ggtcaggcca tggacgacgt tgaatgcctg gatcgcctga
     4441 ccggcggcgc ccttcacgag gttgtcgatc acgccgatcg cgaggatccg gccggtccgc
     4501 tcgtcgaggc gaacgaagac gcgaacctgg ttgctgccga gaacgtgctt ggtggccggc
     4561 gccaccggca cgacggtgac gaagggctcg tcggcgtacg cgctcgcata gaggtcgtcg
     4621 agctccgcct ggctggtcgg gcgggtcggc cggacgtgac aggccgacag gatgccgcgg
     4681 gtcatcggga tcaggtgggg caggaagtcg accgcgccga tgccggggtt ggtcacgccc
     4741 gcctcggccc cgatcccggc caactcctgc tcgatctcgg ccacgtgacg gtgaccgccg
     4801 actccgtacg ccttgacgct ctcgttgacc tcgccgaaca acaggtccgg cttgaggtcg
     4861 cggccggcgc cggagacgcc gctcttggcg tcgacgacga gatccccgat cagcccggcc
     4921 cgagccaggg gagcgagggc gaggagggtg gcggtcgggt agcagccagg tgatccgacg
     4981 atcgccagtg gcgcatggcg aagggcccgg agctcggcac gatgcagctc gggcaggccg
     5041 tagaccgcgc ttccgaggag atcggcccgc ggatgctcga agccgtacca gcggggatag
     5101 tcgagcgcgg cccggaggcg gaagtccgga cccaggtcga tgaccgccgt gccggcggcc
     5161 gcgatctccg gcacgagccc ggcggccgtg ccatggggca gggcgaggaa gaccgcgtcg
     5221 acctcgggca ggtccgatcc gaccgccagc ccggtgcccg acaggtgggg gtggctgacc
     5281 ccgaccggtt cgtgccggtc gcggcccttg aggccgacga tctcgaccgt ggggtgacga
     5341 ccgagcagcc ggatca
//
LOCUS       DATAIW010000027         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149888, whole genome shotgun sequence.
ACCESSION   DATAIW010000027 DATAIW010000000
VERSION     DATAIW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149888"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..359
                     /locus_tag="VHM48_00980"
     CDS             144..359
                     /locus_tag="VHM48_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754003.1"
                     /translation="MSLLSWIKQTLTGAPDVSAAEPPPRHERREAGRERHQETGEPAW
                     EEKDRLDDPPGAGMRDLDAAPEILGGV"
     gene            complement(417..1916)
                     /locus_tag="VHM48_00985"
     CDS             complement(417..1916)
                     /locus_tag="VHM48_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2754004.1"
                     /translation="MQPRTPSDLDAPLTRDQLLVTAGIMAAIAVAALDSTVVGTAMPT
                     IIGQLGGLSEYSWVFTAYLVTSTTTVPLFARLADIHGRKPVFLFGLALFVIGSVLCGT
                     ATSMPALIAFRALQGLGAGAVQPISFTIAGDIFTPRQRARMQGVFSGVWGVSAIIGPA
                     LGGVITTTVGWPWVFEINLPVGILAGAIIWFSFHERFERHPQPIDWLGAILLTGSILL
                     LLFAVSEGGQLFGWASPLVIAMLAGSVVLLVAFVVNARRAAAPLIDLELLRSPLVRAG
                     LGIGGLAGIVIFGLTTYVPLMVQGVHGGTALEAGAAVAAMSIGWPVASVVAGRLLIRI
                     GARPIVLAGTGMLVVGSLLVTQLGRIDALPFAMLACAITGVGMGLSSTTLLVVIQGAV
                     EWQRRAVATGLVQFSRTIGGAVGVGVMGGVLTAFVGATSSAILDPVARGTVSTQAAAA
                     ARDSLSAGLNVTYWLMFGAAALACLLAVRTMPDVSLGHELAAGAGGRAD"
     gene            complement(1948..2643)
                     /locus_tag="VHM48_00990"
     CDS             complement(1948..2643)
                     /locus_tag="VHM48_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2754005.1"
                     /translation="MDELGLGTLDYVYAPSSDVAADARWFSEILGAECVFVIDSDGTR
                     VAMLRAAQIGGARRLLARPSCRHHARGAPTGMPGKIVLMATIPQGAREFLATGPFAHI
                     VTLNPDGTPHVSLAWAGVDDGGIVWSSFSDQRKFDNLRRDPRIVLSFQAHERGDEPLH
                     PYLVIRGRATIEPGGALDVMDQLAEFYIGPGQKFPMRDVPPGLTVHVAVDEVYGQGPW
                     RRSGDGSERPDET"
     gene            complement(2636..3127)
                     /locus_tag="VHM48_00995"
     CDS             complement(2636..3127)
                     /locus_tag="VHM48_00995"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2754006.1"
                     /translation="MRTRAEQIAAIRADQQFWRELAAQVGPDRFAEPGPMGEWTFGDL
                     AGHLVGWRNRTLARLEAFSRGQPDPPDPWPAELGDDDAINDWIHDWHAGRTPGQLVAD
                     YEASYDRLVAVLDAMPNDKLTDPNAIPWVGGPLVDIDFTGHLHDEHVPSVRAWLDASG
                     SHG"
     gene            complement(3297..3653)
                     /locus_tag="VHM48_01000"
     CDS             complement(3297..3653)
                     /locus_tag="VHM48_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754007.1"
                     /translation="MQQESEHVHSTTPREQTFGYGADPDEFDAYLSEVEFSYRREHMR
                     TAALQAGVHWEFLSETNRAAFRSRWAAAQAKIQRVLGQPVSAVVATPTADAYGQRLRD
                     MERSTREAWERAGGGC"
BASE COUNT          605 a         1296 c         1372 g          499 t
ORIGIN      
        1 ccgcgggcga gcgcctcgac gtccagcggc tgcgcgtact cccggtcgat tcgcgctcac
       61 ctcaggaccg cggcggacgg ttgacgcggt gacgcaccat tggggtcgag cgacgccgtt
      121 cgatcgtcgc ggaggagacg ctgatgtcgc tgctgtcctg gatcaagcaa accctgacgg
      181 gcgcgccgga tgtgtcggcg gcggagcccc caccgcgcca cgagcgacgc gaggcgggtc
      241 gcgagcgtca tcaggagacc ggcgaacccg cctgggagga gaaggaccgg ctcgacgacc
      301 cgccgggcgc ggggatgcgc gatcttgacg cggcgccgga gatcctgggc ggcgtctgac
      361 gcgcaggacg tcgtagaaat accaggtcgg caacgagaag cggagcgcgt cagacctcag
      421 tccgcccgcc cgccggctcc cgccgcgagc tcgtgcccga gcgacacatc gggcatcgtc
      481 cggacggcga gcaggcacgc gagtgcggcg gccccgaaca tcagccagta cgtgacattg
      541 aggcccgcgg acagcgagtc ccgggccgcg gcggcggctt gcgtcgacac cgtgccgcgg
      601 gcgaccgggt cgaggatcgc ggacgaggta gcgccgacga acgccgtcag cacgccgccc
      661 atgacgccga ccccgaccgc tccgcctatg gttcggctga actgcaccag gcctgtcgcg
      721 accgcccggc gctgccactc gacggcgccc tggatgacga caagcagtgt cgtggacgac
      781 aggcccatgc cgacgccggt gatcgcacac gcaagcattg cgaagggcag cgcgtcgatt
      841 cgcccgagct gcgtgacgag gagtgatccg acgacgagca tgccggtgcc ggcgaggacg
      901 atcggtcggg cgccgatccg gatcagcagc cggcccgcaa ccaccgacgc gaccggccag
      961 ccgatcgaca tcgcggcgac ggccgcgccc gcctcgagcg ccgtgccgcc gtgcacgccc
     1021 tggaccatca gtggaacgta cgtcgtcagc ccgaatatga cgattccggc cagcccgccg
     1081 atgccgagac cggctcggac gagtggagag cgcaggagtt cgaggtcgat cagtggggcc
     1141 gccgcccgcc gcgcgttgac gacgaaggcg acgagcagca cgaccgagcc ggcgagcatc
     1201 gcgatgacga gcggcgaggc ccagccgaac agctggccgc cttccgacac cgcgaagagg
     1261 aggagcagga tcgatcccgt gagcaggatc gcgccgagcc agtcgatggg ctggggatgg
     1321 cgctcgaacc gctcgtggaa tgagaaccag atgatcgcgc cggcgaggat gccgaccggc
     1381 aggttgatct cgaacaccca tggccacccg accgtcgtcg tgatcacccc gccgagcgcc
     1441 gggccgatga tcgccgacac gccccagacg cccgagaaca caccctgcat ccgggcccgc
     1501 tgtcgcggcg tgaagatgtc tccggcgatc gtgaacgaga tcggctggac ggcgccggcc
     1561 ccaaggccct ggagagcgcg aaacgcgatg agggcgggca tcgacgtcgc ggtcccgcac
     1621 aggaccgacc cgatcacgaa cagcgcgagc ccgaacagga acaccggctt gcgcccgtgg
     1681 atgtcggcga gacgggcgaa gagcggcacg gtcgtcgtgg aggtgacgag atacgccgtg
     1741 aagacccacg agtactcgga cagcccgccg agctgaccga tgatcgtcgg catggcagtc
     1801 ccgacgacgg tcgagtcgag ggccgcgacg gcgatagcgg ccatgatccc ggccgtcacg
     1861 agcagctggt cgcgagtcag cggcgcatcg aggtcgctgg gcgtcctggg ctgcatcctc
     1921 cgagtccagg tcgaaggaga tcgactgcta ggtctcgtcc gggcgttcgg acccgtcgcc
     1981 cgaacggcgc catgggccct ggccgtacac ctcgtcgacg gcgacgtgca cggtgaggcc
     2041 gggcggaacg tcacgcatcg ggaacttctg cccgggcccg atgtagaact ccgcgagctg
     2101 gtccatcacg tcgagcgcgc cgccgggctc gatcgtcgcc cggccacgga tcacgaggta
     2161 cgggtggagc ggctcgtcgc cgcgctcgtg cgcctggaac gacaacacga tccgcgggtc
     2221 gcgccgcagg ttgtcgaact tgcgctggtc cgagaacgac gaccagacga tgccgccgtc
     2281 gtcgacgccg gcccaggcga gcgagacgtg cggtgtccca tcggggttga gggtgacgat
     2341 gtgggcgaac gggccggtcg cgaggaactc gcgcgcgccc tgcgggatcg ttgccatgag
     2401 aacgatctta cctggcatgc cggttggagc gccgcgagca tgatggcggc acgacggtcg
     2461 cgcgagaagc cggcgcgcgc cgcctatctg cgctgcgcgg agcatcgcga cccgcgtccc
     2521 gtccgagtcg atgacgaaga cgcactcggc tccgaggatc tcgctgaacc agcgggcgtc
     2581 ggcggccacg tcggagctcg gtgcgtagac gtagtcgagg gttccgaggc cgagctcatc
     2641 catgcgatcc ggacgcatcc agccacgcgc ggacgctcgg gacgtgctcg tcgtggaggt
     2701 ggccggtgaa gtcgatgtcc acgagcgggc ctccgaccca ggggatcgcg ttcgggtcgg
     2761 tcagcttgtc gttgggcatc gcgtcgagga cggcgaccag gcggtcgtac gaggcctcgt
     2821 agtccgccac cagctgcccg ggcgtgcggc cggcgtgcca gtcgtggatc cagtcgttga
     2881 ttgcgtcgtc gtcgccgagc tccgccggcc atgggtcagg cgggtcgggc tggccccggg
     2941 agaacgcctc gaggcgggcg agcgtccggt tgcgccagcc gacgaggtgg ccggccaggt
     3001 cgccgaaggt ccactccccc atcggccccg gctcggcgaa acgatcgggg ccgacctgcg
     3061 cggcgagctc gcgccagaac tgctggtccg cccggatggc ggcgatctgc tcggccctgg
     3121 ttcgcatcgt tgtctcgcct cgtgttcctg aagggccgcg tcactcgacg gcgagagcaa
     3181 tcctagaccc gcgagctcga gcagcggtca cgcgtggcgc acgcacggcc gggagccagc
     3241 cctgcccgac ccggggacat tcacgttggc ggtgtcgtat gaacgatgct cccggctcag
     3301 cagccgccgc cggctcgctc ccaggcttcg cgagtgctcc gctccatgtc gcgcaatcgc
     3361 tgtccgtagg cgtccgctgt cggcgtggcc accaccgcgc tgacgggctg cccgaggacc
     3421 cgctggatct tcgcctgcgc cgccgcccag cgcgaccgga acgccgcccg gttcgtctcc
     3481 gagaggaact cccaatggac gccggcctgg agcgccgcgg tccgcatgtg ctcgcggcgg
     3541 tacgagaact cgacctccga gaggtaggca tcgaactcgt cggggtcggc gccgtagccg
     3601 aaggtctgct cgcgcggcgt cgtgctgtgg acgtgctcgc tctcctgctg cacgtgccgg
     3661 tactcgtgga ggatgctcgt gtagaggtcg tcgagacgtc gatcagccgg cgaaacgccc
     3721 gcgccgtctc agccgggagg gccgacaccg tggcggccgt gcgcccgtag at
//
LOCUS       DATAIW010000028         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_60586, whole genome shotgun sequence.
ACCESSION   DATAIW010000028 DATAIW010000000
VERSION     DATAIW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60586"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VHM48_01005"
     CDS             <1..701
                     /locus_tag="VHM48_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX2754008.1"
                     /translation="FVLENPVLIIAGALVGASGAILTKLMADAMNRSVLNIMIGGFGG
                     GDTVPGHVGAEGAAVREVGVDDAAIQLAYAQHVIVVPGYGLAVAQAQHAVRELADLLE
                     QRGVDVKYAIHPVAGRMPGHMNVLLAEANVPYPQLKEMDDINPEFERCDVALVIGAND
                     VTNPAARRPGSAVSGMPILDVDKAKSIIVMKRSMGKGYAGIDNELYTNIKTGMLFADA
                     KDGLAALTAAVKAL"
     gene            complement(1190..1888)
                     /locus_tag="VHM48_01010"
     CDS             complement(1190..1888)
                     /locus_tag="VHM48_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX2754009.1"
                     /translation="MPQIDLGLQADLAVRMLVAAVLGAAIGFEREIHEHPAGMRTHLL
                     VSLGSAIFTILSIYGFEGVGGNGVAATVDPTRIAAQIVSGIGFLGAGAILKYGTSIRG
                     LTTAASLWTAAAIGMAAGAGEWLIAAVGTAIVIFSLWPLNALVGRLHKPGSQAIKLRL
                     EVGRLEALGDVSRLLADRRVEMAGINSQRIGKGRYEVELEVRLPTAVRAHDVIAAITA
                     IPDVELMESTTGGE"
     gene            complement(1960..2454)
                     /locus_tag="VHM48_01015"
     CDS             complement(1960..2454)
                     /locus_tag="VHM48_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2754010.1"
                     /translation="MPDQPPTSTPAIDAIAAAGIPHRIVRTERASSAEESATFQGIET
                     RQLLRTIVVRRGEDDYLFVLVPAGRRFDWPKLRELLGVRRMTLPDAAEAHQVTGYERY
                     TITPFGSSRAWPVIADAAIVAEPVVSIGGGGFGINLHLVPADLIDGLGASVADVSQPE
                     PPPA"
     gene            2488..3351
                     /locus_tag="VHM48_01020"
     CDS             2488..3351
                     /locus_tag="VHM48_01020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2754011.1"
                     /translation="MSDPDETRNVRPATPAEAADVRSMHAANRAAWDEAAERYEGWFD
                     EAVALISGGGSNLFPVEHELIGDLRAGAGTARAIHLQCAGGRDTLSLWNLGAREVVGV
                     DISPRMLELAARLTEATGAPARWIEADVLDTPHELDGTADLVYTGRGAIIWLHDLDGW
                     AAVIGRLLSPMGRLVIFDGHPAEWLFDADEDGRLVATDYDYFGGAEASKGWAPEYVDH
                     LSIAEDDQSWKFARAWTLGEIVTALVRAGLRIERLVEYPVDWWAGHRDLNPADRSRVP
                     LSFGIVARPGD"
     gene            complement(3408..3590)
                     /locus_tag="VHM48_01025"
     CDS             complement(3408..3590)
                     /locus_tag="VHM48_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754012.1"
                     /translation="MRGTNFCKPDVFVDGKILKQGGKLVAALDGPRRDVLASRDYLVS
                     TIAEPEPGWLPTLTRA"
     gene            3715..3918
                     /locus_tag="VHM48_01030"
     CDS             3715..3918
                     /locus_tag="VHM48_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2754013.1"
                     /translation="MADKSGFLGSATVGTKGQIVIPADARAAMQIKEGDKVVVLRGPR
                     EGSILVFRVDSFDKLLAKATKAS"
     gene            complement(3943..>4129)
                     /locus_tag="VHM48_01035"
     CDS             complement(3943..>4129)
                     /locus_tag="VHM48_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754014.1"
                     /translation="SVITTVTALAGSILGGVIGAAFGLRAALIVGIAGAVIAVIVVWR
                     SPAGRLRRIEDAEPIAA"
BASE COUNT          643 a         1458 c         1442 g          586 t
ORIGIN      
        1 gcttcgtcct cgagaacccc gtcctgatca tcgccggtgc cctcgtcggc gcgtccggcg
       61 caatcctcac gaagctgatg gccgacgcga tgaaccgctc ggtcctgaac atcatgatcg
      121 gcggcttcgg cggcggcgat accgtcccgg gccacgtcgg cgcggagggc gcagcggtcc
      181 gcgaggtcgg cgtcgacgac gcggcaatcc agctcgccta cgcccagcat gtcatcgtcg
      241 tcccgggcta cgggctcgcg gtggcccagg cgcagcacgc cgtccgcgaa ctggccgacc
      301 tcctcgagca gcgcggcgtc gacgtcaagt acgcgatcca ccccgtcgcc ggccggatgc
      361 cgggccacat gaacgtgcta ctggccgagg cgaacgtccc ctacccccag ctcaaggaga
      421 tggacgacat caatccggag ttcgagcggt gcgacgtcgc cctggtcatc ggcgcgaacg
      481 acgtcacgaa cccggccgcc cggcggcccg gctcggcggt gtcgggcatg ccgatcctcg
      541 acgtcgacaa ggccaagtcg atcatcgtga tgaagcgatc gatgggcaag ggctacgccg
      601 gcatcgacaa cgagctgtac acgaacatca agaccgggat gctcttcgcc gatgcgaagg
      661 acggcctggc cgccttgacc gcggcggtca aggcgctctg acagcgaacc cgtcgacggc
      721 cgcccggcag cccaccggga gatcgccccg gccggacggc gggaggggat cggtacggcg
      781 cgcagtgccc gccgcgcgcg agaatgacgg ctgcgccggg gcccttcgcc ccgcgcggcg
      841 ggccgttcta catggccgga tagtcgtcct cggactcttc gaccaggttc cgggcgctcg
      901 gagcgcgtca ccctgcagat ttccggtcgg agttagccgg attcgactaa cgcctacccg
      961 gcgaggccga acggaacatt cgatacgcgt cccaacagtc ccttcttgaa taactggcca
     1021 ggaatcggcg acgggcgggg cgatcgcgaa gcggaccggc gacgcggcgt cggcgacggg
     1081 ggtcggcgac gggtccagcg cggaccggcg acgggtccag cccagatcct gccggccatc
     1141 cagcgtgtcg ccatgttcat cgctttgcat ccatggcaac cgccctggat cactcgccgc
     1201 cggtcgtcga ctccatcagc tcgacgtccg gaatggccgt gatcgcggcg atgacatcgt
     1261 gggcacggac cgcggtcggg agccgcacct ccagctccac ctcgtagcga cccttcccga
     1321 tccgctggct gttgatcccg gccatctcca cccgccgatc cgccagcagg cgggacacat
     1381 cgcccagcgc ctcgagccgg ccgacctcga ggcgcagctt gatggcctgc gacccgggct
     1441 tgtggaggcg gccaaccagg gcgttcagcg gccagagcga gaagatcacg atcgcggtac
     1501 cgaccgcggc gatcagccac tccccggcgc ctgccgccat cccgatcgcg gccgccgtcc
     1561 agagacttgc cgccgtggtc aggccgcgga tcgaggtgcc gtacttgaga atcgccccgg
     1621 cccccaggaa gccgatcccg gacacgatct gggcggcgat ccgggtcggg tcgaccgtcg
     1681 ctgcgacgcc gttaccaccg acgccctcga agccgtagat cgacaggatg gtgaagatgg
     1741 ccgatccgag cgacacgagg agatgggtcc gcatccccgc cggatgctcg tggatctcgc
     1801 gctcgaagcc gatcgccgca ccgaggacgg ccgcgaccag catccgcacg gcaaggtcgg
     1861 cctgcaggcc gagatcgatc tggggcatgg gcgaacgacc tccggctcgg atcggagcgt
     1921 ggacgccagt ctaggcccgg ccggcatgcg aagttgcatt caggcgggcg gcggctccgg
     1981 ttgactcaca tcggcgacgc ttgcgccgag gccgtcgatc aggtcggccg gcacgagatg
     2041 gaggttgatc ccgaagccgc cgccgccgat cgacacgacc ggctcagcga cgattgccgc
     2101 gtcggcgatc accggccatg cccggctcga cccgaacggc gtgatcgtgt agcgctcata
     2161 gcccgtcacc tggtgggcct ccgccgcgtc cgggagggtc atccggcgca ccccgagcag
     2221 ctcgcgcagc ttcggccagt cgaaccgccg gccggccggc acgagcacga acaggtagtc
     2281 gtcctcgccg cgacggacga cgatcgtccg aaggagctgg cgggtctcga tcccctggaa
     2341 cgtcgcggac tcctcggccg aggacgcccg ctcggtccgg acgatccgat gcgggatccc
     2401 ggcggcggcg atcgcgtcga tggctggcgt ggaggtcggc ggctggtcgg gcatgccgag
     2461 atggtagcca gcgctagact ccggcgaatg tccgatcccg acgagacccg caacgtccgc
     2521 ccggcgacgc cggccgaggc cgctgacgtc cgatcgatgc acgccgcgaa ccgggcggcc
     2581 tgggacgagg cggccgagcg ttacgagggc tggttcgacg aggccgtggc gctcatcagt
     2641 ggcggcggct cgaacctctt cccggtcgag cacgagctga tcggcgacct ccgggcgggc
     2701 gccggcaccg cgcgggcgat ccatctccag tgcgcgggcg gccgcgacac cctgtcgctt
     2761 tggaatctcg gtgctcgaga ggtcgtcggc gtcgatatca gcccgcggat gctcgagctc
     2821 gccgcccggc tgacggaggc gaccggtgcc ccggcccgct ggatcgaggc cgacgtcctc
     2881 gacacgccgc acgagctcga cgggacggcc gacctcgttt acaccggccg cggcgcgatc
     2941 atctggctcc acgacctcga cggctgggcg gcggtcatcg gccgccttct ctccccgatg
     3001 ggtcggctcg tgatcttcga cggccacccg gccgagtggc tgttcgacgc cgacgaggac
     3061 ggccgcctcg tcgccaccga ttacgactac ttcgggggcg ccgaggcgtc gaagggctgg
     3121 gcgcccgagt acgtcgacca cctgtcgatc gccgaggacg accagagctg gaagttcgcc
     3181 cgggcgtgga cgctcggcga gatcgtcacc gccctcgtga gggcgggcct acggatcgaa
     3241 cgtctcgtcg aataccccgt cgactggtgg gccggccacc gcgacctgaa ccctgccgat
     3301 cgcagtcggg tccccctgtc gttcgggatc gtcgcccggc ccggtgactg agctcggccg
     3361 tggacctccg gagggttcgt ccgccgccgg ctggagggtt cgacgggtca ggcccgggtg
     3421 agggtcggca gccagccggg ctccggctcg gcgatcgtgg acacgaggta gtcgcgtgag
     3481 gcgagcacgt cgcgacgcgg accgtcgagc gcggcgacaa gcttgccgcc ctgcttgagg
     3541 atcttcccgt cgacgaagac gtcgggcttg cagaagttcg tgccgcgcaa gggccatcgt
     3601 gacgagggcc cgatcgtcgc tcagccgggt ccgcaccttg cgggccaggt cgcctgtccg
     3661 ctatccttcg tgcaactggt atgacttgtc atactcagtt ggactggagg taacatggcg
     3721 gacaagtcgg gcttccttgg ctctgcgacg gtgggcacca agggccagat cgtcatcccc
     3781 gccgacgcac gcgcggcgat gcagatcaag gagggcgaca aggtcgtcgt cctgcgcggt
     3841 ccgcgcgagg gaagcatcct cgtgttccgg gtcgactcgt tcgacaagct cctggcaaag
     3901 gcgaccaagg ccagctgagc cgggacaggc tgcgctggcg cgtcaggcgg cgatcggctc
     3961 ggcgtcctcg atgcgccgga gccggcctgc cggcgagcgc cacacaacga tcaccgcgat
     4021 gaccgccccg gcgatcccga cgatcaacgc cgctcgcagc ccgaaggccg cgccgatgac
     4081 cccgccgagg atcgagccgg caagcgcagt gacggtcgtg atgacgctc
//
LOCUS       DATAIW010000029         8178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_149928, whole genome shotgun sequence.
ACCESSION   DATAIW010000029 DATAIW010000000
VERSION     DATAIW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149928"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VHM48_01040"
     CDS             <1..448
                     /locus_tag="VHM48_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX2754015.1"
                     /translation="ADLIVLGPGSLYTSLLPSLLLPQIRDAVLASPALRLYVCNVATQ
                     DGETADLDLAGHVEALVAHAGPGIVDVVLANNRPGDPWSLRPPSEPVRLTWPPSLAVA
                     PRVVLDDVVDSGAAQRHDPDRLAAAVMRILEREGTARRRGSASRSA"
     gene            504..1412
                     /gene="whiA"
                     /locus_tag="VHM48_01045"
     CDS             504..1412
                     /gene="whiA"
                     /locus_tag="VHM48_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX2754016.1"
                     /translation="MSGSDRDLVLALRSELAALDPARPCDRRAEAIALADVGAARDGP
                     LARLLLRLARERAAVQAAAGDEAPGAIDPAAGGIRRFDWAASADHCRLAWLRGMFLAH
                     GSLSLSGGRTHLEFLVAAPDAELLARWLDQADLPASWRIRRGRGVVTWKNGDLVGTFL
                     RRIGAIGALLEVEARQVSRAMRGDLNRVINAESANLHRAVGAAGRQIAAIDELTASGE
                     LAEQPFVIRSVAEARHETPEATFAELAERLGIHRSAVQRALERIERLARMPDDDERRR
                     RRMRRSREPAGPRSAPAPAAIGRPLA"
     gene            1420..2214
                     /gene="tpiA"
                     /locus_tag="VHM48_01050"
     CDS             1420..2214
                     /gene="tpiA"
                     /locus_tag="VHM48_01050"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2754017.1"
                     /translation="MRPVVIAANWKMNTTPSDAGELAVTIAGRTRVEGVTRVICPPFV
                     AIAAVRDALAAADPGVGVGAQDVHHELAGAFTGDISAPMLVGLAGWVIVGHSERRRDH
                     AETDERIGRKLARAVEAGLRPILCVGEQLEDRDAGRAVTVVDAQLAGALVGHDPAALA
                     EAGLVIAYEPVWAIGTGRNALGSDAAAMAEAIRATLARLGWDASAGEVPILYGGSVTA
                     ANIGEFLAEPSIDGALVGGASLKPDEMAGIVGRAGVTAAARMAAMS"
     gene            2231..3865
                     /gene="gpmI"
                     /locus_tag="VHM48_01055"
     CDS             2231..3865
                     /gene="gpmI"
                     /locus_tag="VHM48_01055"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX2754018.1"
                     /translation="MTPGAVRPRPIVLVIIDGFGLGPVPADDAIAAAAMPTWRGLLDR
                     WPHCILRASEDAVGLPPGQMGNSEVGHLNLGAGRPVLQDLPRIDAAIADGSFFERPAL
                     SAACERAARTGGRLHIVSLIGPGGVHANDRHLVALVELAARSNVGEVRIHALLDGRDT
                     PPRSALDFVRDLEARLAVAHPDARIATIGGRYYAMDRDQRWERTARGYAAIVHGAGER
                     APSATAAIEAAYARGENDEFVLPTVIDGVDGRVRDGDAMVHANFRADRARQLTHALAE
                     GDEFTAFDRDVPAARPRDVLVVTMTAYESGLPVEVVFGPEEVRSLAHAASDAGWRQFH
                     VAETEKYAHVTYFFNGGREAPLPGEERRLIPSAKVATYDLQPEMRAAGVTDALIEAIE
                     SDAYDLIVANFANPDMVGHTGVWDATVRAVETIDGCLARIVAVLGGPDPAGPGSLLAI
                     TADHGNADAMRDAAGNPVTAHSLNPVPLLLAGRLVADRTLHDGVLADVAPTLLELAGL
                     PRWAGMTGRSLLDPAEGIEPEPVLSSGAVPTGGNPS"
     gene            3862..4086
                     /gene="secG"
                     /locus_tag="VHM48_01060"
     CDS             3862..4086
                     /gene="secG"
                     /locus_tag="VHM48_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2754019.1"
                     /translation="MNAPLAIGQIIVSIALIAAILLQARGTGLSGTFGGDSAVYRSRR
                     GIEKRLWQFTIVLLVLFVAFSVGSYLYQNI"
     gene            4147..5880
                     /locus_tag="VHM48_01065"
     CDS             4147..5880
                     /locus_tag="VHM48_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2754020.1"
                     /translation="MNRIDRVVIAGLVLVLAIVAIAIGGPALAPKPAPEPTSAGPSSA
                     APEPYREGVLSRPTNVNPLAARTQADRDLVALVFEGLVARTADDKPTPALARSWTSTA
                     TGDAWTFELGPDRFWQDGEPVTADDVVFTIETLQDPDYHGPGSGSWAGITVSAVDAAT
                     VRFELATPIGGFLELATQPIVPRHLLGDTAPGAMADAAFGKEPIGSGPYTVAELDRDH
                     AVLERAPDATTPPTGGGASPAPAGDPLATPVPTHRADDLQVGLARLEFRFFDDADSLA
                     TAFRNGELDVVSGLDPAAAEALAATPGARAIRNPSTTLAAVVLNLHPTEEAFADPRTR
                     LALLEAVDRARLVGVVYGGAAVLADGLIPPSSWAFDTAASPPIGRDLKAAAKSLTAAG
                     WTKAKDGWHHGGAKEARTIQLLVPNRTANPILFAAGSQIAADWTALGFTVSIVEEDPA
                     IIATDHLRTGDFEAALVDIAIGHDPDLYALLASSQIRTGGANLIGLQDPLLDDLLEKA
                     RKPAADKARLAAYKALQERLAGGTYLLPMAWPNKVVVVAERVVGPAVREVADGSERFG
                     DVLTWRLADDR"
     gene            5911..5997
                     /locus_tag="VHM48_01070"
     tRNA            5911..5997
                     /locus_tag="VHM48_01070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5945..5947,aa:Leu,seq:cag)
     gene            6037..6121
                     /locus_tag="VHM48_01075"
     tRNA            6037..6121
                     /locus_tag="VHM48_01075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6071..6073,aa:Leu,seq:gag)
     gene            6189..6262
                     /locus_tag="VHM48_01080"
     tRNA            6189..6262
                     /locus_tag="VHM48_01080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6223..6225,aa:Val,seq:tac)
     gene            complement(6508..7788)
                     /locus_tag="VHM48_01085"
     CDS             complement(6508..7788)
                     /locus_tag="VHM48_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754021.1"
                     /translation="MDTEEMALGDDVPHAPERGPDIANRRSRRAVLAGAFGAVVGAAV
                     GRLAQPPSVRAATGDPVLAGQANTANTETDLTSTDVNYSNAVLNVSGITGRTIAAVNT
                     ATSPDGIGGNGVVGTSSVGFGVVGSGGRGGVIGSSFLGPGIWGTASYDSPAVLGEISG
                     PSSRPAVFGHATNNGLGSNAAGPGVYGVSRPSSTDHPTPPVNTGVAGWSFAGTGARGD
                     SDTGVGTSAQTTGGNLAAQAFTGAGDPPTPAPIAYAIPTATYGEANGTNGTGVWGYSA
                     ASTGTGVYGESSIGVWGFGGWGVFGASDATGTGVYGFSGSTVPAAPAHTGVFGYSDSG
                     YGVYARALTGTALYVNGKARFSRSGVISITSGHRSITKTLSGVTASSMIIAMAQNLVT
                     GVFVQAAIPGSGKFTIYLNKTVTATTKVAWFVIN"
BASE COUNT         1137 a         3022 c         2803 g         1216 t
ORIGIN      
        1 agcggacctg atcgtcctcg gtccggggtc cctgtacacg agcctcctgc cgagcctgct
       61 cctgccccag atccgcgacg ccgtgctcgc ctcgccggcg ctccggctct acgtctgcaa
      121 cgtcgccacg caggacggcg agaccgccga tctggacctc gccggtcacg tcgaggcgct
      181 ggtcgcccac gccgggccgg ggatcgtcga cgtcgtcctc gccaacaacc ggcccggcga
      241 tccgtggtcg ctccggccgc cgtccgagcc ggttcgcctg acgtggcccc cgtcgctcgc
      301 ggtggcgccc cgcgtcgtgc tcgacgatgt cgtcgacagc ggcgcggccc agcgccacga
      361 cccggaccgg ctggctgcgg cggtgatgcg gatcctcgag cgggagggca cggcacggcg
      421 acgcggctcc gcctcgcgct cggcctgatc gagcccgtgt tccgcccctt ccgacccacg
      481 ccgcgccgga tcgacggccg gccgtgagcg gcagcgaccg cgacctcgtg ctggccctgc
      541 ggtccgagct cgcggcgctc gacccagcgc ggccctgcga ccgacgggcc gaggccatcg
      601 cactcgccga cgtcggggcc gcgcgcgatg gacccctcgc ccggttgctg ctccgactcg
      661 cgcgcgaacg agccgcggtc caggcggcgg ccggcgacga ggcgcccggc gccatcgacc
      721 cggccgcggg cgggattcgc aggttcgact gggcggcatc ggccgatcat tgccggctcg
      781 cctggctgcg cggcatgttc ctggcccacg ggtcgctcag cctgtccggt ggtcggaccc
      841 acctcgagtt cctcgttgcc gcgccggacg ccgagctgct ggcccgctgg ctggaccagg
      901 cggaccttcc ggcgtcgtgg cggatccgcc gtggccgcgg cgtggtgacg tggaagaacg
      961 gcgatctcgt cggcacgttc ctgcgccgga tcggggcgat cggggccctc ctggaggtcg
     1021 aggcgcggca ggtctcgcgg gccatgcgcg gcgatctcaa ccgggtgatc aacgcggagt
     1081 cggcaaatct ccatcgagcg gtcggggcgg ccggccgcca gatcgccgcg atcgacgagc
     1141 tgaccgccag cggcgagctt gccgagcagc ccttcgtcat ccgctcggtc gccgaagccc
     1201 gccacgagac gcccgaggcg acattcgcgg agctggcgga gcggctcggg atccaccggt
     1261 cggcggtcca gcgcgcgctc gagcggatcg agcgactcgc ccggatgccc gacgacgacg
     1321 aacggcgccg acgccggatg cgccggtcca gggagcctgc cgggccccgg tccgcgccgg
     1381 ctcccgcggc gatcggccgg cctctggcat gatcactcca tgcgccctgt cgtgatcgcc
     1441 gccaactgga agatgaacac cacgccgtcc gacgccggcg agctggccgt gacgattgcc
     1501 ggccggacgc gcgtcgaggg cgtcacgcgg gtcatctgcc cgcccttcgt cgccattgcc
     1561 gccgtgcgcg acgccctcgc cgccgccgac ccgggtgtcg gcgtcggggc ccaggatgtc
     1621 catcacgagc tggccggggc cttcacgggc gacatctcgg caccgatgct ggtcggcctg
     1681 gccgggtggg tgatcgtcgg ccactccgaa cgccggcggg accacgccga gaccgatgag
     1741 cggatcggac gcaagctcgc ccgggccgtg gaggccggcc tccggccgat cctgtgcgtg
     1801 ggcgagcaac tcgaggaccg cgatgccggc cgggccgtta ccgtggtcga cgcgcagctc
     1861 gccggtgcgc tcgttggcca cgatccggcg gcgctcgccg aggccgggtt ggtcatcgcc
     1921 tacgagccgg tctgggcgat cgggacgggc cgcaatgcgc tcggctccga tgccgcggcg
     1981 atggcggagg cgatccgggc gacgctcgcc cgtctcggct gggacgcctc cgcgggcgag
     2041 gtcccgatcc tctacggcgg gagcgtcacg gccgcgaaca tcggcgagtt cctggccgaa
     2101 ccgtcgatcg acggggccct cgtcggcggc gcgtcactca agccggacga gatggccggg
     2161 atcgtcggcc gggccggcgt gaccgcggca gcccgaatgg cggccatgtc gtgacgccgg
     2221 tcgtcacccc gtgacaccgg gcgccgttcg acctcggccg atcgtcctgg tcatcatcga
     2281 cggcttcggt ctgggaccgg tgcccgccga cgacgcgatc gccgccgccg cgatgccgac
     2341 ctggcgaggt ctcctcgatc gctggccgca ctgcatcctg cgcgcgtcgg aggatgcggt
     2401 cgggctgccg ccgggccaga tgggcaactc ggaggtcggc cacctgaacc tcggtgccgg
     2461 ccggccagtg ctccaggacc tgccgcggat cgatgccgcg atcgccgacg ggtcgttctt
     2521 cgagcggccg gcgctcagcg cggcctgcga gcgggccgcc cggaccggcg gccggctcca
     2581 catcgtcagc ctgatcggcc cgggcggcgt ccacgccaat gatcggcatc tcgtcgcgct
     2641 ggtcgagctg gcggcccgat ccaacgtcgg agaggtccgg atccacgcgc tgctcgacgg
     2701 ccgcgacacg ccgccccgct ctgcgctcga cttcgtgcgc gatctcgagg ctcgcctcgc
     2761 ggtcgcccat cccgacgccc ggatcgcgac gatcggtggc cgctactacg cgatggaccg
     2821 cgaccagcgc tgggagcgga ctgcccgcgg ctacgcggcg atcgtccacg gggcagggga
     2881 gcgggcgccg tcggcgacag ccgcgatcga ggcggcctac gcccgcggcg agaacgacga
     2941 gttcgtcctg ccgaccgtga tcgacggtgt cgatggccgg gtccgcgacg gcgacgcgat
     3001 ggtccacgcc aacttccggg ccgatcgcgc ccggcagctc acgcacgccc tcgcggaggg
     3061 cgacgagttc acggcgttcg atcgcgacgt gccggcagcc cggccgcgcg acgtgctcgt
     3121 cgtgacgatg accgcgtacg agtcgggcct gccggtcgag gtcgtgttcg gaccggagga
     3181 ggtccgctcg ctggcccacg ccgcgagcga cgccgggtgg cgccagttcc acgtcgcgga
     3241 gaccgagaaa tacgcccacg tcacctactt cttcaatggc ggccgggagg cgccgttgcc
     3301 gggcgaggag cgacgcctca tcccgagcgc caaggtcgcc acctacgacc tccagccgga
     3361 gatgcgcgcg gccggcgtga ccgacgccct gatcgaggcg atcgagagcg acgcctacga
     3421 cctgatcgtc gccaacttcg ccaacccgga catggtcggc cataccggcg tctgggacgc
     3481 cacggtgcgg gcggtcgaga cgatcgatgg ctgtctcgcc cggatcgtcg ccgtcctcgg
     3541 cggccccgat cccgccggcc ccggcagcct cctcgcgatc accgccgacc atggcaacgc
     3601 cgatgcgatg cgcgacgccg ccggcaaccc ggtgacggcc cactcgctca atcccgtccc
     3661 tctcctgctc gcgggccgcc tggtggccga ccggacgctc cacgacggcg tcctcgccga
     3721 cgtcgccccg acgttgctcg agctcgccgg gctcccacgc tgggcgggga tgaccggccg
     3781 atcgctgctg gacccggcgg aggggatcga accggagccc gtgctatcat ccggcgccgt
     3841 tcccaccgga ggaaatccct cgtgaatgcc cccctggcca tcggccagat catcgtcagc
     3901 atcgccctca tcgccgccat cctgctccag gcccggggca ccggcctgtc cggcacgttc
     3961 ggcggcgact ccgcggtcta tcgcagccgg cgcgggatcg agaagcgcct gtggcagttc
     4021 acgatcgtgc tgctcgtgct gttcgtggcg ttctcggtcg ggtcctacct ctaccagaac
     4081 atctgagctc gctcgggtct gtcgcggcgc ccggggaacg acacgccgaa gacccagcac
     4141 cacccgatga accggatcga ccgcgtcgtc atcgccgggc tcgtcctggt cctcgccatc
     4201 gtcgcgatcg cgatcggcgg gccggcgctc gcacccaagc cagcgcccga accgacgagc
     4261 gctggcccgt cctcggccgc gcctgaaccg taccgcgaag gcgtgctctc ccggccgacg
     4321 aacgtgaacc cgctcgccgc gcgaacccag gccgatcgcg acctggtcgc cctggtgttc
     4381 gagggactcg tcgcccggac cgccgatgac aagccgacgc ccgcgctcgc tcgatcgtgg
     4441 acctcgaccg ccaccggtga cgcctggacg ttcgagctcg gacccgatcg tttctggcag
     4501 gacggcgagc ccgtcaccgc cgacgacgtc gtcttcacga tcgagacgct ccaggatccg
     4561 gactaccacg gaccgggatc cgggtcgtgg gccgggatca cggtcagcgc cgtcgatgcc
     4621 gccaccgtcc gtttcgagct cgcgacgccg atcggcggct tcctcgagct ggcgacccaa
     4681 ccgatcgtcc cgcgccatct gctcggcgac accgcgccgg gcgcgatggc ggacgccgcg
     4741 ttcggaaagg aaccgatcgg ctcgggtcct tacacggtcg ccgagctgga ccgcgaccac
     4801 gcggtcctcg agcgggcgcc cgacgcgacg acgccgccga ccggcggcgg ggcgtcgccg
     4861 gccccagcgg gagacccgct cgcgaccccg gttccgaccc accgcgcgga cgatctccag
     4921 gtcgggttgg cgcgcctcga gttccgcttc ttcgacgatg cggacagcct ggcgacggcg
     4981 ttccggaacg gcgagctcga cgtcgtatcc ggcctcgacc cggctgcggc cgaggccctc
     5041 gcggccacgc ccggcgcccg cgccatccgc aatccgtcga cgaccctcgc cgcggttgtc
     5101 ctcaacctcc acccgacgga ggaagccttc gccgatcccc ggacccggct cgccctgctc
     5161 gaggcggtcg atcgggcgcg gctcgtcggc gtcgtgtacg gcggcgccgc cgtgctggcg
     5221 gatggcctga tcccgccatc atcgtgggcg ttcgacacgg ccgcgagccc accgatcggg
     5281 cgcgacctga aggccgccgc gaagtccctc acggccgccg gctggacgaa ggccaaggac
     5341 ggctggcatc acggcggcgc gaaggaggca cgaacgatcc agctgctcgt cccgaaccgg
     5401 accgcgaacc cgatcctctt cgcggccggc agccagatcg cggccgactg gaccgccctc
     5461 ggcttcaccg tgtcgatcgt cgaggaggat cccgcgatca tcgccaccga ccacctccgg
     5521 accggcgact tcgaggcggc gctggtcgac atcgcgatcg ggcacgaccc ggatctctac
     5581 gcgctcctcg cctcgagcca gatccggacc ggcggggcga atctcatcgg gctccaggat
     5641 ccgctcctcg acgacctcct cgagaaggcc cgcaaacccg ccgccgacaa ggcccggctg
     5701 gccgcctaca aggccctcca ggagcggctc gcgggcggga catacctcct cccgatggcg
     5761 tggccgaaca aggtggtcgt cgtcgccgaa cgggtcgtcg gaccggccgt ccgggaggtc
     5821 gccgacgggt cggagcgatt cggtgatgtg ctaacatggc gcctcgctga cgaccggtga
     5881 gccctcgggc aggccggccg gcgggtcgac gccgaggtgg cgaaattggc agacgcgcta
     5941 gcttcaggag ctagtgcccg caagggcgta tgggttcaag tcccttcctc ggtaccacct
     6001 cctccctccc gacccgtcgc gacgaacggc acacctgccc aggtggcgga attggtatac
     6061 gcgtacgttt gaggggcgta tgaggcaact catccgggtt caagtcccgg cctgggcacc
     6121 aacctcgcac cgatcacgac cgccggccga ccggcggtcg cttcatgtct gcggctatgc
     6181 tgtcgggcgg gcggttagct cagttggtca gagcgtctcg tttacaccga ggaggtcact
     6241 ggttcgagcc cagtaccgcc cactggccgc ccgggatgca tgaacatcgc cgcctggctc
     6301 gctcaggtgc cggcatcggg cggaatcgtc cgttggtgcg gaagcgatgg gcgtcggcca
     6361 tgccgccgga cgcgtacgtc accgagtctg tctctgccgt gtcctttcgg cttggaagga
     6421 aagtgcgtgg gttgcggcgc gcgtcgatgg ctcttcgggc ctgtcggcgg cccgcccggt
     6481 gcggccccgt cgaattcgtt cgacatctca gttgatcacg aaccaggcga ccttggtcgt
     6541 cgccgtcacg gtcttgttca ggtagatcgt gaacttgccg ctccccggga tcgccgcctg
     6601 gacgaagact cccgtgacca ggttctgagc cattgcaatg atcatgctgc tggcagtaac
     6661 gccgctcaga gtcttcgtga tcgagcgatg gcccgacgta atcgatatga cgccgcttct
     6721 cgagaacctg gccttgccgt tgacgtatag cgcggtgcca gtcaacgccc ttgcatagac
     6781 tccgtacccg ctgtccgaat agccgaagac gccggtgtgg gccggcgcag cgggtacggt
     6841 ggaacctgag aagccgtaca cgccggttcc cgttgcgtcg ctcgcgccga acactcccca
     6901 gccgccgaat ccccagacgc cgatcgacga ttcgccgtaa acgccggtgc cggtcgaggc
     6961 agcgctgtaa ccccagacac cggtcccgtt cgtcccattc gcctcgccat aggtcgcggt
     7021 cgggatcgcg tatgcgatcg gtgccggcgt cggaggatcg cccgcaccgg tgaatgcctg
     7081 tgccgcgagg tttccgcccg tcgtctgcgc agatgttccc actccagtgt cgctgtcgcc
     7141 tcgcgcaccg gttccggcaa agctccagcc cgccacaccg gtgttgaccg gcggcgtcgg
     7201 atgatcggtc gacgacggac ggctgacgcc gtagacccca ggtccagcag cgttgctgcc
     7261 cagcccgtta ttggtcgcgt ggccgaagac cgccggacgc gaagacggac cggagatctc
     7321 ccccagcacg gcgggcgagt catagctggc ggtaccccag atccctgggc ccaagaacga
     7381 ggagccgata acacctccgc gtccgccgga acccacgacc ccaaatccca cggagctcgt
     7441 gccgaccacc ccgttgccgc cgatcccgtc tggcgaggtc gccgtgttca cggccgcaat
     7501 cgttcgtccg gttattccgg agacgttgag aacggcgttg ctgtagttga cgtcggtact
     7561 ggtcaggtcg gtctctgtgt tggccgtgtt tgcctgcccg gcgagaacgg gatctcctgt
     7621 ggctgcccgg acgctgggag gttgggccag ccgaccgacg gccgcaccga cgacggctcc
     7681 aaaggcaccc gcaagcacgg cgcgtcgcga ccgacgattc gcgatatcag gccctcgctc
     7741 gggagcatgg ggcacgtcat cccccaacgc catctcctcg gtgtccatcc cacggctcct
     7801 ctcgcctcgt ggcactgcgg ccgcgtcgcc cttctgcgac ggcttcgcat gtgcaactca
     7861 atgaacgccc gggcatccga tccgttacga ggcgaccgac gacgggcggg cgatgggccg
     7921 ctttcggaca ctcctcagcc gtgatcagct gtcgcccggt cctcagccag caggacccga
     7981 ctggtagact gatcaggcgg gggcaaggat gccttccatc gaccgaccgt agagggtgag
     8041 catgaatcca gcattgatca ttgtcctggt ggtcatcgtc gtcgcggtga tcttcgtgat
     8101 cgcgctctac aacggcctgg tccagcgacg gctccggatc gacgaggcct tcgcccagat
     8161 cgaggtccag ctcaagcg
//
LOCUS       DATAIW010000030        11530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_90443, whole genome shotgun sequence.
ACCESSION   DATAIW010000030 DATAIW010000000
VERSION     DATAIW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90443"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /gene="pknB"
                     /locus_tag="VHM48_01090"
     CDS             complement(<1..1690)
                     /gene="pknB"
                     /locus_tag="VHM48_01090"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX2754022.1"
                     /translation="MAEIGTILGGRYRLVELLGQGGMARIYRGHDNQLGRDVAVKILR
                     PEYGRDPDFSSRFRQEAQNAASLNHPNIVGVYDYGQDEAGPFIVMELVDGEDLGAIIK
                     RSGALPPRQAARITAETARALHAAHQRGIVHRDVKPGNVMINRDGQVKVTDFGIARAM
                     AEAQMTLPGTTLGSVHYFSPEQARGDQTTAASDIYSLGIVLFELLTGHRPFEADSAAA
                     VAMARLAGPPPDPSTLRSGIPADLVAIDRKALATEPADRWSAASSMAAALEAFLAGTT
                     VPGMTGATGASAPTMASGGAGAAGAAGVGAVGVAGAGLAATSATARANPRAIPYVPEA
                     YVDDARPRRSDVYARPPGQPGGRDRGGPPVRPVDEDDPPGGTSPAVWAAGIVALLILA
                     LVGFLVFRLLSGGGATPVAQITVPDFVNKTLVQAQTLAAQAGISVSPTATAAPSGVVA
                     NTVLSQDPAAGTKIDKGGTVAIVVAAGAETVAVPDLRNRTEIDAFNLLAAAGLSFGPK
                     TEAFDPIVSLGSVISQNPAPGVLVNKGTAISYVLSKGPEPSPSPSPSPTPTPTPP"
     gene            complement(1871..2410)
                     /locus_tag="VHM48_01095"
     CDS             complement(1871..2410)
                     /locus_tag="VHM48_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754023.1"
                     /translation="MTLEVLVIGAVRVLGSLPVLRWPLAGGILAILVDLSDLLLRDTL
                     DLGGIPDYQAFDKWADQVYLGLFLVVALRWSGVERSIAVGLYVFRLVGFVAFELSGER
                     VLLLVFPNVFEPWFLTVAAVHHWRPGFAWRPATVVAGLATLTALKEVQEWALHGARLF
                     DGISSLEFIDRVRRWLAGG"
     gene            complement(2635..3513)
                     /gene="galU"
                     /locus_tag="VHM48_01100"
     CDS             complement(2635..3513)
                     /gene="galU"
                     /locus_tag="VHM48_01100"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX2754024.1"
                     /translation="MRVRKAVFPAAGWGTRFLPATKAQPKEMLPLVDKPVIQYAVEEA
                     VASGIEHVIIVTSSQKRAIEDHFDTSYELERLLEEKGDIEMLRQIRRISDLAQVTYVR
                     QKEQLGLGHAVLVAKEIVGHEPFAVILSDDVVIGDRPCIGQLVHAYNQTHSSVVAVME
                     VPHEETQRYGVIAGEEVEGWLNHGRLHKVSRLVEKPRPEDAPSNLAIIGRYVLTPKIF
                     DKLEQTQRGAGGEIQLTDAIEALMQEQAVYGYEFEGTRYDCGTTMGWLKASVELALQR
                     PELAPEFRAYLRDLEL"
     gene            complement(3643..4404)
                     /locus_tag="VHM48_01105"
     CDS             complement(3643..4404)
                     /locus_tag="VHM48_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2754025.1"
                     /translation="MTRSKRPAELTAPLDDARPTIVFLHGTRLTGAEWAAQVATLGDA
                     FHCLTPDLPGHGAASDVPFTVAAAAGRVADLIEREAHGGRAILVGLSLGGYVAMAVAA
                     SWPERVVGLVISGATAEPVGLRSLAYRGLAAIFGAMPEPILDGVNRWFFGWRFPPAIA
                     DPILAAGFSFAGGAVAVRSLVGERFKPLLAAYPGPSLLINGEFDLFFRTSERAFADVA
                     ADPRRALIRRATHLTNLDEPERFSSLVRAFATRIG"
     gene            complement(4413..5072)
                     /locus_tag="VHM48_01110"
     CDS             complement(4413..5072)
                     /locus_tag="VHM48_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2754026.1"
                     /translation="MPETGEAARIRVLLVDDHGVVRRGLRGYLELLDDIEVVGEAENG
                     LIGVELSARLVPDVVLMDIVMPGLDGIGAIGRIKAAQPGVQIVALTSFIEEDKVLAAL
                     ESGASGFILKDADADDVAAAIRAAYHDEVHLDPAAARVLAKGIRTRVAQQPVESLTER
                     ELEVLSLVGHGRSNKEIATDLGITERTARTHVSNILGKLDLQSRTQAALYAVERKLVH
                     D"
     gene            complement(5145..6104)
                     /locus_tag="VHM48_01115"
     CDS             complement(5145..6104)
                     /locus_tag="VHM48_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2754027.1"
                     /translation="MRDRRFLYGSLALILAATGFGMLGPIARLAYEAGFEPMAFVAWR
                     ATFGLLAIVAIILAQTRRGIPVADPRGLHRRDLAGLVLIAVTGLGVNISTFVAFDLTT
                     VALVLLAFYTYPALVAVVAFALGHERLDGARLLALGLALGGMVLVVLGGLDPARGGVG
                     FNPIGVLLGLAAAASQTVFVTVSRGRFAAVSSEQAMAWVLLLIAAATIPLAGVLGDPL
                     DVPFHDGRALGLALLAGILGAGLPSVLFLVGIRAIGGTRAGILMLIEPLVGVTLAALL
                     LQEALLPIQVVGGAAILAAALLLQRGSPNDGREPVLVPLAEHT"
     gene            complement(6165..7283)
                     /locus_tag="VHM48_01120"
     CDS             complement(6165..7283)
                     /locus_tag="VHM48_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754028.1"
                     /translation="MAETWIPATTSALALVFAVALLDQWRERRQTFQLIWAVGMLFYG
                     VASGCEAIGAASGWNEALYRTWYLAGAVWTAGWLGLGTAFLLGRTRFGYTFAFCLLLA
                     GVFTLLTERKFHYPGSGSAPILYLIGAIVLAAAVAIETYFQNERWPRLAAGAVGGATI
                     LSLVLMLTVSLPAPGYALDPATGAPTGDLLPGTIRLLTPFLNITGGLALVLGALFSAY
                     VFMPKRRVLGYSLDPTAPGDQFLFNLLISPVAIVVNFVASVPGAVRALFTGRLHSRVP
                     ATILIAIGGLFASGGDALNRFGVTNYFQVGKFLAVIFLLAGFLVSIETFREIRIPFTA
                     IRLGARRTEHDLAGGNALPGGSGAPTAAAASSTDHATG"
     gene            complement(7286..8317)
                     /locus_tag="VHM48_01125"
     CDS             complement(7286..8317)
                     /locus_tag="VHM48_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754029.1"
                     /translation="MDMNVALPFASSTLSFVFGAFLLDQWRTRRRPYQLIWAIGMLWY
                     ALSAGTEFLGGAVGWSEPLYRAWYLIGAVWVAGWLGLGTVYLLGRTRFGYAFAVALGL
                     AGLFTFLTWRKYDYPSSGIAPFVYLGVAIGLAVVVAVMTRRHDERWVHVAGAVVVGGS
                     VVSALMALTASLPAPGWIVDRATHIPTGDLFPGYLRLLTPFFNITGAFVLVFGALYSA
                     YMFMPKQRLVRYERDQKGAAGFIVGLLVAAAAISINFVASLPRAVASLLTGRLNSRVP
                     ATILIAIGGLIPAITSGANRFGDTSGFFVGELLGVLFLFLGFLVSIEVFHDFRVPFTR
                     RVIRDRAEA"
     gene            complement(8319..8987)
                     /locus_tag="VHM48_01130"
     CDS             complement(8319..8987)
                     /locus_tag="VHM48_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754030.1"
                     /translation="MPAAIRSVRSVRSVSDPPLSLGPASRLLGVDPDTLRRWADEGRI
                     EAFTTAGGHRRFHRATVERILQARRHDATVKLASLGATEDRLARAYRRGYLSTSDVGD
                     VRDAIPAVDRDAFRDGGRALVAAMLAHLDAPDDAARDHAAATAVHLTEDLARRLAAAG
                     IPLGEAVSLFVAARRPFLTELGVIARHRSLDPDRLAAIYDSSSGLLDRLLLRLVAVHQ
                     EVST"
     gene            complement(9166..9528)
                     /locus_tag="VHM48_01135"
     CDS             complement(9166..9528)
                     /locus_tag="VHM48_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754031.1"
                     /translation="MDIMGIRIPTVVQDNVARRCDGCLEVIEGTPWRVNLLDIVTTEV
                     AAPWTDTSAINPGPFEFHSDDACVRRWMASRDFLFCRKGRVREIMRPIPVPGPDGASV
                     RWGLCDGIHRDDHELIPA"
     gene            complement(9599..10555)
                     /locus_tag="VHM48_01140"
     CDS             complement(9599..10555)
                     /locus_tag="VHM48_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754032.1"
                     /translation="MRKRLAPLVSLVIAAVVLTAGCAGAAAPPDAYQQLTTSMKTTWN
                     PIQVNVGLTVTAAGKTVTLDPADIAIVIDAAGGKGAVHISLPVSDLGIPDSALLQLGI
                     DGDSIEFDMVYAGDALYARSAMFKPVLKLVLGPAGKLPAGDLTGWLKLGTKDELAALA
                     AISGSGAMPSVVPSSDENAATKASLEAAGITLTIVGTEKRNGADLQHLKVAVDMAKLA
                     TNPSFLAGAGGGAQAAQTAAMVKALSFTGDLWMDPTTHRIVESSAHLASTTDASQFGD
                     VSVTVRDPDGSVSLDAPASSIAIPIGPLVTEMMKLISNGAES"
     gene            complement(10961..>11530)
                     /locus_tag="VHM48_01145"
     CDS             complement(10961..>11530)
                     /locus_tag="VHM48_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2754033.1"
                     /translation="FNDAKSGARMVDLNGQLTMVAGQHVGYVTVLMGGNDVCQPTEAQ
                     MTSVADFTAQFTTAMATITAASPTTLVYVVSIPNVYNLWSTLKNNGIARFVWGPFGVC
                     QSMLANPLSTAKADVRRRARVLRREVDFNTALAKVCAAHVQCRFDGNAVFNVTFSASD
                     ISTRDYFHPSLAGQTKLASVSWAAGYWKP"
BASE COUNT         1841 a         3872 c         4253 g         1564 t
ORIGIN      
        1 gtggaggggt cggcgtcggc gtcgggctgg gggacgggct cggcgagggc tcggggccct
       61 tcgagaggac gtacgagatc gcggtgccct tgttgacgag gaccccgggt gcggggttct
      121 ggctgatgac cgagcccagc gaaacgatcg ggtcgaacgc ctcggtcttc ggcccgaacg
      181 acaggccggc ggcagccagg aggttgaatg cgtcgatctc ggtccggttg cggagatccg
      241 gcacggcgac ggtctcggcc cccgccgcga cgacgatcgc gaccgtgccg cccttgtcga
      301 tcttcgtgcc cgcggccggg tcctggctca gcacggtgtt cgcgacgacg ccgctcgggg
      361 cagcggtcgc ggtcggactg acggagatgc cggcctgggc cgcgagggtc tgggcctgga
      421 cgagcgtctt gttcacgaag tccgggaccg tgatctgggc gaccggggtc gcgcctccgc
      481 ccgacaacag gcggaagacg aggaacccga ccagggccag gatgaggagg gcgacgatcc
      541 cggcggccca gaccgcgggc gatgtgccgc ccggcgggtc gtcctcgtcg accggacgga
      601 cgggcggccc gccgcggtcc cggccgccag gttggcccgg cgggcgcgcg tacacgtccg
      661 agcggcgcgg tcgtgcatcg tcgacgtagg cctcgggaac atacgggatg gctcgcgggt
      721 tcgcgcgggc ggtcgccgac gtcgcggcga gaccggcgcc ggccacaccg accgcgccga
      781 cgcctgcggc gcccgcagcg cctgcaccgc cggacgccat cgtcggggcg gaggcgccgg
      841 tggccccggt catccccgga acggtggtcc cggccaggaa cgcctcgagc gcagcggcca
      901 tcgacgaggc ggccgaccag cggtcggccg gctcggtcgc gagggccttg cgatcgatcg
      961 cgacgaggtc ggccgggatc ccggaccgga gggtcgacgg gtcgggcggc ggaccggcga
     1021 gacgggccat cgccaccgcc gcggcgctgt ccgcctcgaa cggacggtgc ccggtcagga
     1081 gctcgaacag gacgatcccg agcgaataga tgtcggacgc cgccgtcgtc tggtcgccgc
     1141 gggcctgttc ggggctgaag tagtggacgg acccgagtgt cgtcccaggc agcgtcatct
     1201 gcgcctcggc catggcccgg gcgatcccga agtcggtcac cttgacctgg ccgtcccgat
     1261 tgatcatcac gttgccgggc ttcacgtcgc ggtggacgat cccgcgttga tgggccgcgt
     1321 ggagggcgcg ggcggtctcg gcggtgatgc gggccgcctg ccgcggcggc agcgcccccg
     1381 agcgcttgat gatcgcgccg aggtcctcgc cgtcgacgag ctccatgacg atgaacgggc
     1441 cggcctcgtc ctggccgtag tcgtacacgc cgacgatgtt cgggtggttc agcgaggccg
     1501 cgttctgggc ctcctggcgg aagcgcgagc tgaagtcggg atcacggcca tattcgggcc
     1561 tgaggatctt gacggcgacg tcgcgtccga gctggttgtc gtggccgcga tagatccggg
     1621 ccatcccgcc ctggccgagc agctccacga gccgataccg gccgccgagg atggttccga
     1681 tctccgccaa gtcagcctcc gctgcgggtt ctggcccgaa cgccgcgacc gctgccggca
     1741 ttcccgggcg gtgacgcacc cgtcggcatc gtagcgtacg acgctcgagc gagtggtcgg
     1801 tgaggccggg tcgccccgtt cggccaacgc cacgaaatcc gcgcgcgtcc gcagggcgct
     1861 ccgtcgtcgg tcaaccgccg gcgagccagc gccggacccg atcgatgaac tcgagcgagc
     1921 tgatcccgtc gaacaggcgg gcgccgtgca gcgcccactc ctggacctcc ttgagcgcgg
     1981 tcagcgtcgc gagcccggca acaaccgtcg ccggccgcca ggcgaacccc ggccgccagt
     2041 ggtggacggc agcgaccgtc aggaaccacg gctcgaaaac gttcggaaag acgaggagca
     2101 acacccgttc gcccgacagc tcgaaggcga cgaagccgac gagccggaac acgtagagcc
     2161 cgaccgcgat cgagcgctcg acgcccgacc agcgcagcgc cacgacgagg aacagcccga
     2221 gatagacctg gtctgcccac ttgtcgaacg cctggtagtc ggggatgccc ccgagatcga
     2281 gcgtgtcgcg gagcagcagg tccgagaggt ccacgaggat cgccaggatc ccgccggcga
     2341 gcggccagcg caggaccggc agcgagccga ggacccggac cgccccgatg acgaggacct
     2401 cgagcgtcac gagtgcgggc tcatcatgtc gtgccctcgc agtcttgccg cgcgccgcat
     2461 ctcgggcggg taccgaccga ccggcgccga cgcgcgatgt cgcccatgga tcaattccgg
     2521 atgagtgagc gcggcccaat tgcgggtgcg accgaacgcg tcgggcgggt gattcggtcg
     2581 cgcttgttcg tgtggatctg atccatcacc ggcatcacgc cgagcagatc ggccttacag
     2641 ctccaggtcg cggaggtaag cgcggaactc gggggccagc tcgggacgct ggagggccag
     2701 ctcgaccgac gccttgagcc agcccatcgt cgtgccgcag tcgtagcggg tcccctcgaa
     2761 ctcgtagccg tagacggcct gctcctgcat cagggcttcg atggcgtcgg tcagctggat
     2821 ctcgccgccg gcgccgcgct gggtctgctc gagcttgtcg aagatcttcg gagtcagcac
     2881 gtagcggccg atgatcgcga ggttcgacgg cgcatcctct ggcctcggct tctcgaccag
     2941 ccggctgacc ttgtgcaacc ggccatggtt gagccagccc tcgacttcct cgcccgcgat
     3001 cacgccgtag cgctgcgtct cctcgtgggg cacctccatg accgcgacca ccgagctgtg
     3061 cgtctggttg taggcgtgaa cgagctggcc gatgcagggt cgatcgccga tgacgacgtc
     3121 gtcggacagg atgaccgcga agggctcgtg gccgacgatc tccttggcga cgaggaccgc
     3181 gtgcccgaga ccgagctgct ccttctggcg gacgtaggtg acctgggcga ggtccgagat
     3241 ccgccggatc tggcggagca tctcgatgtc gcccttctcc tcgagcagcc gctccagctc
     3301 gtaggaggtg tcgaaatgat cctcgatcgc tcgcttctgg ctcgaggtga cgatgatcac
     3361 gtgctcgatg cccgaggcga cggcctcctc gaccgcgtac tggatcacgg gcttgtcgac
     3421 gaggggcagc atctccttcg gctgggcctt ggtcgcgggg aggaagcggg tcccccatcc
     3481 ggcggcgggg aatacggcct tgcgaacgcg catgaaacct cggtccatca cgggaagagc
     3541 ggtcgggcga ggataccatc ggggtccggt tcgaccgtcc gggggctcgg ccgccggtcc
     3601 gggcgcgagg cgaccggagc ggcggcgaag ggtgatcggc ggtcagccga tcctggtcgc
     3661 gaacgcccgg accaggctgc tgaaccgttc cggctcgtcg aggttcgtca ggtgcgtggc
     3721 ccgccggatc agggcccggc gcgggtcggc ggcaacgtcc gcgaaggccc gctcgctcgt
     3781 gcggaagaag aggtcgaact ccccgttgat caggaggctc ggcccggggt atgccgccag
     3841 cagcggcttg aaccgctcgc cgacgagcga ccgcaccgcg acggcgccgc ccgcgaacga
     3901 gaacccggcg gcgagaatcg ggtcggcgat cgccggtggg aaccgccagc cgaagaacca
     3961 gcggttcacc ccgtcgagga tcggctcggg catggcgccg aagatcgcgg ccaggccgcg
     4021 gtaggccagg gaccgcagcc cgaccggctc ggcggtcgcg cccgagatca ccaggccgac
     4081 gacgcgctcc ggccagctcg ccgcgaccgc catcgcgacg tatccgccga gcgacaggcc
     4141 gacgaggatc gcccggccgc cgtgcgcctc gcgctcgatg aggtcggcga cgcgtccggc
     4201 agcggcggcg accgtgaacg gcacgtccga cgcggcgccg tgaccgggga ggtcgggggt
     4261 caggcaatgg aaggcgtcgc cgagggtcgc cacctgggcg gcccactcgg ccccggtcag
     4321 gcgcgtcccg tgcaggaaga cgatcgtcgg gcgcgcgtcg tcgagcggtg ctgtcagctc
     4381 ggcggggcgc ttcgatcggg tcatcgtgac cctcagtcgt ggaccaattt gcgttccacg
     4441 gcgtacagcg ccgcctgggt ccggctctgg agatcgagct tgccgaggat gttgctgacg
     4501 tgcgtccggg cggtgcgttc ggtgatcccg agatcggtcg cgatctcctt gttgctccgg
     4561 ccgtggccga cgaggctgag aacctcgagc tcgcgctcgg tcaggctctc gaccggttgc
     4621 tgcgcgaccc gcgtccggat ccccttggcc aggacccggg cggccgccgg gtcgaggtgg
     4681 acctcgtcgt gatacgcggc ccggatcgcc gcggcgacat cgtccgcgtc ggcgtccttg
     4741 aggatgaagc cgctcgcgcc cgattcgagg gcggccagca ccttgtcctc ctcgatgaag
     4801 ctcgtcaggg cgacgatctg gaccccgggc tgggcggcct tgatccgacc gatcgccccg
     4861 atcccgtcga gcccgggcat gacgatgtcc atgaggacca cgtcgggcac gagccgggcc
     4921 gacaactcga ccccgatcaa cccgttctcc gcctcgccga cgacctcgat gtcgtcgagc
     4981 agctcgagat agccccgcag cccgcgccgg acgacgccgt ggtcgtcgac cagcaggaca
     5041 cgaatgcgtg ctgcctcgcc cgtctcaggc atcgactcca cctcccttcc gatcatcgca
     5101 tccaccgatc gctcgccacc gttgcccatc gtggccgatc ggcgtcacgt gtgctcggcc
     5161 agcgggacga ggacaggttc ccgtccatcg ttcggggaac cccgctggag caggagcgcg
     5221 gcggcaagga tcgcggcgcc gccgacgacc tggatcggca gcagggcctc ctgcagcagg
     5281 agagcggcga gcgtcacccc gacgagcggc tcgatcagca tgaggatccc ggcccgggtc
     5341 ccgccgatcg cccggatccc gacgaggaag aggaccgacg gcaggccggc cccgagaatc
     5401 cccgccagca gggcgagacc gagcgcccga ccgtcgtgga acgggacatc gagcggatcg
     5461 ccgaggacgc cggccagcgg gatcgtggcg gcggcgatca acagcagcac ccaggccatc
     5521 gcctgctccg aggacacagc cgcgaaccgc ccgcggctca ccgtgacgaa gacggtctgg
     5581 ctggccgccg cggcgagccc gagcaggacc ccgatggggt tgaatccgac gccgcccctg
     5641 gccggatcca ggccgcccag gacgacgagg accatgccac ccagcgccag cccgagggcg
     5701 agcaggcgcg cgccgtcgag ccgctcgtga ccgagtgcga acgcgacgac cgcgaccagc
     5761 gcggggtacg tgtagaacgc gagcaggacg agggcgacgg tcgtcaggtc gaacgcgacg
     5821 aacgtcgaga tgttgacgcc aagtccggtg accgcgatca gcacgagccc ggcgaggtcg
     5881 cgccgatgga ggccacgggg atcggcgacc gggatcccgc gccgggtctg ggcgaggatg
     5941 atcgcgacga tcgcgagcag cccgaatgtg gcccgccacg cgacgaacgc catcggctcg
     6001 aagccggctt cataggcaag tctggcgatc ggcccgagca tgccgaatcc ggtcgccgcg
     6061 aggatcaggg caagcgatcc gtacaggaag cggcgatcgc gcatcgggga atggtacgag
     6121 ccgcgggtcc ggggtcggtt tcgggaggac ccgtggcgtt cggatcagcc ggtcgcgtga
     6181 tcggtggacg aagccgcggc cgccgtcggc gctccggatc cgcccggcag ggcattcccg
     6241 ccggcgaggt catgctccgt ccggcgggcc ccgagccgga tcgcggtgaa gggaatccgg
     6301 atctcgcgga aggtctcgat cgagacgagg aagccggcca gcaggaagat gacggcgagg
     6361 aacttgccga cctggaagta gttcgtgacc ccgaaccggt tcagagcgtc accaccgctc
     6421 gcgaacaggc ctccgatcgc gatgaggatc gtcgccggga cgcggctgtg gagccggccg
     6481 gtgaacaggg cccggacggc gcccggcacc gaggcgacga agttgacgac gatcgcgacc
     6541 ggcgagatca gcaggttgaa gaggaactgg tcgccggggg cggtcgggtc gagggagtag
     6601 ccgaggacgc gccgcttggg catgaagacg taggccgaga acagggcccc gaggacgagg
     6661 gcgagcccgc cggtgatgtt gaggaatggc gtcagcaacc ggatcgttcc gggcaagagg
     6721 tcgccggtcg gcgccccggt cgccggatcg agcgcgtagc ccggcgccgg gaggctgacg
     6781 gtcagcatca gcaccagcga caggatcgtc gccccgccga ccgctccggc ggcgagccgc
     6841 ggccagcgct cgttctggaa atacgtctcg atcgcgacgg cggccgcgag gacgatcgcc
     6901 ccgatgaggt agaggattgg cgcggagcct gagccggggt aatggaactt gcgctcggtc
     6961 aggagggtga agacaccggc cagcaggagg cagaacgcga acgtgtaccc gaatcgggtc
     7021 cggccgagga ggaacgccgt cccgagcccg agccagccgg ccgtccagac cgcgccggcg
     7081 aggtaccacg tccggtagag cgcctcgttc cagccactgg ccgcgccgat cgcctcgcag
     7141 cccgacgcga cgccgtagaa cagcatcccg accgcccaga tcagctggaa ggtctggcgt
     7201 cgctcgcgcc actggtcgag cagggcaacg gcgaagacca gggcgagggc ggaagtcgtg
     7261 gcggggatcc aggtttcggc caacgtcagg cctccgcgcg gtcgcggatg acgcggcgcg
     7321 tgaacggaac gcggaagtcg tggaacacct cgatcgagac gaggaacccg aggaacagga
     7381 acaggacccc gagcaattcg ccgacgaaga agccgctggt gtccccgaat cggttggcgc
     7441 cgctggtgat cgccgggatg aggccgccga tcgcgatcag gatcgtcgcg ggcacgcggc
     7501 tgttgagtcg accggtcagc agcgaggcca ccgcccgcgg aagagaggcg acgaaattga
     7561 tcgagatcgc ggcagccgcg accagcagtc cgacgatgaa cccggccgcg cctttctgat
     7621 cccgctcgta gcggaccagg cgttgcttcg gcatgaacat gtacgcgctg tagagcgccc
     7681 cgaacacgag cacgaacgcg ccggtgatgt tgaagaacgg ggtcagcagg cggaggtagc
     7741 cggggaacag atcgccggtc gggatgtggg tcgcccggtc gacgatccag cccggcgcgg
     7801 gcagcgacgc cgtcagggcc atcaacgcgg acacgacgga accgcccacg acgaccgcgc
     7861 cggcgacgtg cacccaccgc tcgtcatggc ggcgggtcat gaccgcgacg acgaccgcca
     7921 gtccgattgc caccccgaga tacacgaacg gggcgatacc gctcgacgga tagtcgtatt
     7981 tccgccacgt caggaacgtg aagagcccgg ccaggccgag cgccaccgcg aacgcatacc
     8041 cgaaccgggt ccggccgagg aggtagaccg tgccgagacc cagccagccg gcgacccaga
     8101 cggcgccgat caggtaccag gctcggtaca acggctcgct ccagccgacg gcgccaccga
     8161 ggaactcggt gccagccgac agggcgtacc agagcatccc gatcgcccag atcagctggt
     8221 aaggccggcg gcgtgtccgc cactgatcga gcaggaatgc gccaaagacg aagctcaggg
     8281 tggacgatgc gaacgggagc gcaacgttca tgtccatgtc aggtcgagac ctcctggtgg
     8341 accgcgacga gccgcagcag gaggcggtcg agcaggccgg acgagctgtc gtagatggcc
     8401 gcaagccgat cgggatcgag cgagcggtgg cgggcgatga cgccgagctc cgtcaggaac
     8461 gggcggcggg ccgcgacgaa gagcgacacc gcctcgccga gtgggatccc ggccgcggcc
     8521 agccggcggg cgaggtcctc ggtcaggtgg acggcggtcg cggcggcatg atcgcgagcg
     8581 gcgtcgtcgg gcgcgtcgag gtgggcgagc atcgccgcaa cgagggcgcg tccaccgtcg
     8641 cggaacgcgt cgcgatcgac cgccgggatg gcgtcgcgga catcgccgac atcggacgtc
     8701 gacagatagc cgcgtcgata ggcccgcgcg aggcggtcct cggtcgcgcc caggctggcc
     8761 agcttgacgg tggcgtcgtg gcggcgtgcc tggaggatgc gctcgactgt cgcccggtgg
     8821 aaccggcggt ggccgccggc cgtggtgaac gcctcgattc gcccttcgtc ggcccagcgc
     8881 cggagggtgt cggggtcgac gccgagaagc cggctggcag gcccgagcga gagcggcggg
     8941 tcggagacgg agcgcacgga acggacggag cggatggcgg caggcacggc gatgcgcgtc
     9001 ctctggacag aatgtgggca aatctgggtc ggtcgatcag accgtcgagg cgcatgatcg
     9061 cagccgttca ttcgggcgtc aacctcgggc cgatggcgtc gcggcgctcc gcctcggacc
     9121 tcggtgggtc ggccgcaggt tcgttctcgc agacgcggtc cagggtcagg cggggatcag
     9181 ctcgtggtcg tcgcgatgga tcccgtcgca caggccccag cgaacggatg cgccgtccgg
     9241 gccgggcacc gggatcggcc gcatgatctc gcggacccga cccttccggc agaagaggaa
     9301 gtcccgcgag gccatccagc gccggacgca ggcatcgtcg gaatgaaact cgaacgggcc
     9361 ggggttgatc gccgacgtgt ccgtccaggg ggcggcgacc tcggtcgtca cgatgtccag
     9421 gaggttgacc cgccagggcg tgccctcgat cacctccagg cagccgtcgc agcggcgggc
     9481 gacgttgtcc tggacgacgg tcgggatccg gatccccatg atgtccatcg cggcgatggt
     9541 agcaggcagc gttccggccg acggcctcgg tcaggccggc gaccgggccg ccgacgggtc
     9601 agctttcggc gccgttgctg atcagcttca tcatctcggt gacgaggggt ccgatcggga
     9661 tggcgatcga cgacgccgga gcgtcgagcg acaccgatcc gtccgggtcg cgcaccgtca
     9721 cgctcacgtc gccgaactgc gacgcatccg tcgtcgatgc caggtgcgcg ctggactcga
     9781 cgatccggtg cgtggtcggg tccatccaca ggtcgcccgt gaacgagagc gccttgacca
     9841 tcgccgctgt ctgggcggcc tgggcgccgc caccggcgcc ggcgaggaac gaggggttcg
     9901 tcgcgagctt ggccatgtcg accgcgacct tgaggtgctg gaggtccgcg ccgttgcgct
     9961 tctcggtccc gacgatcgtc agggtgatcc ccgcggcctc cagcgaggcc ttggtcgccg
    10021 cgttttcgtc agaggacggg acgacgcttg gcatcgcacc gctgccggag atggcggcga
    10081 gggcggcgag ctcatccttc gttccgagct tgagccagcc cgtcaggtcg ccggccggga
    10141 gcttcccggc cgggccgagg accagcttca gcaccggctt gaacatcgcg ctccgggcgt
    10201 acagcgcgtc gcccgcgtag accatgtcga attcgatcga atcgccgtcg atgcccagct
    10261 ggagcaacgc cgagtcggga atgcccagat cggacacggg cagcgagatg tggactgctc
    10321 ccttgccgcc ggcggcgtcg atcacgatcg cgatgtccgc cggatcgagg gtgaccgtct
    10381 tgccggccgc ggtgacggtc aggccgacgt tgacctggat cggattccag gtcgtcttca
    10441 tcgaggtcgt cagctgctgg tacgcgtccg ggggtgctgc cgcgccggcg caaccggcgg
    10501 tcagcacgac cgcggcgatg acgagcgata caagcggggc gaggcgtttg cgcacgtcgt
    10561 aggccctcgt tgcggactgc cggcccggcg cgatcgcgac cgggtcggcg acaccatacc
    10621 gcgcggggca acgcgcgcca tcagggccga tcggcaggtc gcaggctgac gggtagcgcc
    10681 aagcgtagcc cacccggcat ccctcgttgt ggagcgccag agtcgcgtag ccgacgctcg
    10741 ggccgtctcg gagggcaaga gcgtgacgtc gttcagcggt cgacgcagcg ctggtgcccc
    10801 cgacacgacg cgcacattgt ggccggcgtc gtcgctggag cctgtgctcg ccccgcatcc
    10861 gagacgcgac tcccagacgg tccggcgccc tcgaactagt ggaactcccc accgagcgct
    10921 tgcagcgccg gcagggccgc gcctccagcg acgcgccggc ctacggtttc cagtagcccg
    10981 cggcccagct caccgaggcg agcttcgtct gtcccgcgag cgacgggtgg aagtagtccc
    11041 gggtcgagat gtcgctcgcg ctgaaggtga cgttgaacac ggcattgccg tcgaagcggc
    11101 attggacatg tgcggcgcag accttggcga gggccgtgtt gaagtcgact tcgcgccgca
    11161 ggacgcgagc ccgccggcgc acgtcggcct tggcggtcga gagcgggttg gcgagcatgg
    11221 actggcatac gccgaagggc ccccacacga atcgcgcgat gccgttgttt ttgagggtgg
    11281 accacaggtt gtacacgttc gggatgctga cgacgtacac gagcgtcgtc ggggaggcgg
    11341 ccgtgatcgt cgccatcgcg gtcgtgaact gggcggtgaa atcggccacc gaggtcatct
    11401 gcgcctcggt cggttggcag acatcgttgc cgcccatgag gacggtgacg tagccgacat
    11461 gctgcccggc gaccatcgtc agctggccgt taaggtcgac catccgggca ccgctcttgg
    11521 cgtcgttgaa
//
LOCUS       DATAIW010000031         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_150062, whole genome shotgun sequence.
ACCESSION   DATAIW010000031 DATAIW010000000
VERSION     DATAIW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150062"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VHM48_01150"
     CDS             <1..593
                     /locus_tag="VHM48_01150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2754034.1"
                     /translation="HRAARVSAGPDRPLVLYQGGFSVDRGIEELVAALETPALAGTDV
                     VAVFIGYGRLRTWLDEQAARLPGRIAVVDAIPPGDLQEYTVDADVGFVGQPPRTLNQR
                     LNLANKLFEYLQAGVPVIVAEGTAHCRLVRQLEVGHCVEVDDPSTIARAVVELIGAAP
                     DERERLRLHARTVALEQYTWEVQGVNVIELYRGLGR"
     gene            590..>1691
                     /locus_tag="VHM48_01155"
     CDS             590..>1691
                     /locus_tag="VHM48_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2754035.1"
                     /translation="MSRTAADSGPAGGRADRIAIVTHSVFEEDPRIRREAEALVGAGW
                     QVDIFALRGPGDPAAEALGIRTVPIDVGRHQGAGLLTYLIEYSSFLVRAGWALARAHR
                     RRRYRVVQVAAPPDPLVFAALPLRLIGVPIVLDLHEATPEFFKSRFPRASNPLSDRLL
                     HLAERVSIGLARVVISVNQARHARLLALGYRPDKLRVVTNGPSLGRFRPEAHPVRPFM
                     ADGTLRIVYTGAVTPLYELDMVVRAIGRIRDERPDLPVTFEIYGRGDAEPGLTELAAG
                     LGIADRIVFHGRIPLEDVAAALAAVDIGVSPIRRDPFTEISMPTKALEYAVMDKPVVA
                     ADLPAARQHFDGPMLSWYEPGDAASLAAAPGSY"
BASE COUNT          211 a          636 c          599 g          245 t
ORIGIN      
        1 cccatcgagc cgctcgcgtg tcggcaggcc cggaccggcc gctcgtgctc taccagggcg
       61 gcttcagcgt cgaccgcggc atcgaggagc tcgtcgccgc cctcgagact ccggccctcg
      121 ccgggaccga tgtcgtcgcc gtcttcatcg gttacggccg gctgcggacg tggctcgacg
      181 agcaggcggc gcgcctcccc gggcggatcg cggtcgtgga tgcgatcccg cccggggacc
      241 tgcaggaata caccgtcgat gccgacgtcg ggttcgtcgg ccagccgccg cggaccctca
      301 accagcggct gaacctcgcc aacaagctgt tcgagtatct ccaggcgggc gtgcccgtga
      361 tcgtcgccga gggcacggcc cactgccggc tcgtccgaca gctcgaggtc gggcactgcg
      421 tcgaggtcga cgacccgtcg acgatcgcgc gggccgtcgt ggagctgatc ggggccgcgc
      481 cggacgagcg tgagcggctg cgtctccacg cgcggaccgt ggcgctcgag cagtacacct
      541 gggaggtcca gggcgtgaac gtgatcgagc tgtaccgagg gctcggccga tgagccggac
      601 cgcggcggac agcgggcccg caggcggtcg ggccgatcgg atcgccatcg tgacccacag
      661 cgtgttcgaa gaggaccccc ggatccggcg cgaggccgag gcgctcgtcg gcgccggctg
      721 gcaggtcgac atcttcgccc tccgcgggcc cggcgacccc gcggccgagg ccctggggat
      781 ccggacggtc ccgatcgacg tcggccgcca ccagggggcc gggctcctca cctacctgat
      841 cgaatattcg tcgttccttg tccgtgccgg ttgggcgctc gcccgggccc atcgtcggcg
      901 gcgctaccgc gtggtccagg tggcggctcc gccggacccg ctcgtcttcg cggcccttcc
      961 gctccgcctg atcggggtgc cgatcgtcct cgatctccac gaggcgaccc cggagttctt
     1021 caagagccgc ttcccgcggg cctcgaaccc gctgtccgac cgcctgctcc acctcgccga
     1081 acgcgtctcg atcgggctcg cgagggtcgt catctcggtc aaccaggccc gccacgcccg
     1141 gctgctggcc ctcggctacc ggccggacaa gctgcgggtg gtgacgaacg ggccgtcgct
     1201 ggggcggttc cggcccgagg cgcacccggt ccgcccgttc atggccgacg gcacgctccg
     1261 gatcgtctac acgggcgcgg tgacgccgct gtacgagctc gacatggtcg tccgcgccat
     1321 cggccggatc cgcgacgagc gacccgacct gccggtcacc ttcgagatct acggtcgcgg
     1381 cgacgccgag cccgggctga ccgagctcgc cgccgggctc gggatcgccg accgcatcgt
     1441 cttccacggc cggatcccgc tcgaggacgt ggcggccgcg ttggcggccg tcgacatcgg
     1501 cgtctcgccg atccggcgcg acccgttcac cgagatcagc atgccgacca aggccctcga
     1561 atacgcggtc atggacaagc ccgtggtggc cgcggacctg ccggcggcgc gccagcattt
     1621 cgacggtccg atgctgtcct ggtacgagcc gggcgacgcg gcgagcctgg ccgcggcgcc
     1681 cggctcgtac c
//
LOCUS       DATAIW010000032         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_150071, whole genome shotgun sequence.
ACCESSION   DATAIW010000032 DATAIW010000000
VERSION     DATAIW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150071"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..796)
                     /gene="hisB"
                     /locus_tag="VHM48_01160"
     CDS             complement(173..796)
                     /gene="hisB"
                     /locus_tag="VHM48_01160"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX2754036.1"
                     /translation="MTTPLGSLEVVAREGRRVTVRRVTRETDVRVTVDLDGSGTADIR
                     TGVGFYDHLLGSLAHHGLLDLEIRATGDLEVDDHHTVEDVALVLGSAIAEALGDRAGI
                     VRFGDASVPMDEAVATAIIDVGGRPYAVVDLPFRGERAGGLTLQLVEHALEAFARTAG
                     ATLHVRGTGRNDHHLAEAAFKALGRALRAATEPEPRRTGIAATKGST"
     gene            complement(793..1950)
                     /locus_tag="VHM48_01165"
     CDS             complement(793..1950)
                     /locus_tag="VHM48_01165"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2754037.1"
                     /translation="MSPSPVTAVTFGMPTHPASYSWEATDAEVAARYGIPIEQILRFD
                     LNTSPAPPDFVAGILAAGTFDAPLSEYPPSDYRRLVETAAGVYGVGTDELLVGAGADE
                     ILDLAGKAFLAPGSLAVVPAPTYAMYRIVTEQRGATAVLVPRLGAGAGYAVDPGPTRE
                     AAVDAELVWLCSPNNPTGLPEPAGAIATLLDGIAADAQRAGRAPAIVVLDEAYAEFVG
                     ASLLDLRLDHPNLVVVRTASKAYALAGLRVGFAIARPETIARIAPYRPPGSVSTISVT
                     VVTEALADPRGMRANVERVDAERDRLSGALRDAGWAVGPTVTNFLLVDFGTTDRAAAT
                     ATGLLGRGLVPRTFGAGHPLAHCLRLTIRDRAGNDRLIAAAGEIEGAGTTR"
     gene            complement(1947..3329)
                     /gene="hisD"
                     /locus_tag="VHM48_01170"
     CDS             complement(1947..3329)
                     /gene="hisD"
                     /locus_tag="VHM48_01170"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2754038.1"
                     /translation="MTTSASLDPLAATVRLRRLDLSSTAAADIRERAALCRRGAVPDD
                     AVRSGARSILADVRTRAGAAVVEAGARFGGALADGRLVIDRGELRAARDRLAAPDRRA
                     LDQAIEHVAKFAATQRPATTRTGIVPGIEIERRWLPLDRVGCYVPGGSAPYPSSLIMT
                     VVPARVAGVAGIVVASPADASGAVHPVLLGVAGLLDVDTLLVAGGAQAIGALAFGLRD
                     TTPPLAAVDRIVGPGNAWVTAAKLEVAGAVGIDLPAGPSEGMVLADDTADPVLVAADL
                     VTQAEHGPDSPAILVTTSLAVADAVEREVAALLPRLERRDILARALASHGRIALAPDL
                     DAAIAFVDAYAPEHLSIDVRDPDAAAARIRHAGSIFVGPWAPESAGDYATGANHVLPT
                     GGLARSADPLAVEAFGRWTQIQRVDERGLAAIRETVGRLATAEGLTAHRNAVEARFRD
                     RSDAATGVDR"
     gene            complement(3326..4201)
                     /gene="hisG"
                     /locus_tag="VHM48_01175"
     CDS             complement(3326..4201)
                     /gene="hisG"
                     /locus_tag="VHM48_01175"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2754039.1"
                     /translation="MSALEGRERLRLAIPNKGRLVEPTVELLRDAGLVFEEHERSLVA
                     RVRNFDLDILFVRTNDVIEFVGDGVADLGVTGEDLLAETGAELPRLRSLGYGQCRLAA
                     AVPADSALRELDDLAGLRVATAHPNTARRFFAAAGIAVDVIPISGAVEVAPRLGLAEA
                     IVDLVSTGSTLVMNGLRPIADVLASEAILVANPTAVVQRATQIDAIDTMLSAVIAARG
                     RKYLMMNAPAVRLRDLEALLPGLESPSVIPLAHAGMIAIHAVVGADDVWSLLPRLKAA
                     GASGILVLPIEKIVP"
     gene            complement(4198..4524)
                     /locus_tag="VHM48_01180"
     CDS             complement(4198..4524)
                     /locus_tag="VHM48_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX2754040.1"
                     /translation="MDDWRSSDVDELISAVLRLETADEAASFLRDLCTLGELRDLAQR
                     WAVVRRLDAGEHYAAISRETGASTATITRIASWLNHGEGGYRAALERLAAAREEGIPY
                     PTRAER"
     gene            4603..5367
                     /locus_tag="VHM48_01185"
     CDS             4603..5367
                     /locus_tag="VHM48_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX2754041.1"
                     /translation="MSSQFPSTFTILPAIDLRGGRVVRLKQGDFAREQVYADDPLAVA
                     REFAAAGAGWIHIVDLDGARAGERRETSVISALVALLRGLGSRVQVAGGLRTAQAVAD
                     VLEAGTERVVIGTAALRDPAFVEAACARHGPPRIAVALDVRDGDAIGDGWVAGAAGVP
                     VVDALSRLAAVGVTTFAVTAIERDGLLDGPDLALLERCVRSTDAAVLASGGIRSVDDL
                     EAVGRIGCSGAIVGRAIYDGSLDLAAAISAIGATAI"
     gene            complement(5453..>5686)
                     /locus_tag="VHM48_01190"
     CDS             complement(5453..>5686)
                     /locus_tag="VHM48_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754042.1"
                     /translation="GRVLGMDTLGDGQQVVSAQVPQSELFSYATELRSLTHGRGTFTA
                     VVDHYEEVPGHVAEKVIDAHRKGAEATGAHGGH"
BASE COUNT          779 a         1952 c         2196 g          759 t
ORIGIN      
        1 gcgccgacgc ccgggacgac gagcgcgtcg gcgccggcca gaccggtcgg gtcgcgagcg
       61 acgacgacct cggctccgac acgggtcagg gcctggtcga tgctgacgag gttgccggcg
      121 ccgtagtcga cgacggcgaa ccgcggccgt cgggtcacgt cgagctcctc ggtcacgtcg
      181 agcccttcgt cgcggcgatc ccggtccggc gcggttccgg ctcggtggcg gcgcgcagcg
      241 cgcggccgag cgccttgaac gcggcctcgg cgaggtgatg gtcattgcgg cccgtgcctc
      301 ggacgtggag ggtcgccccg gccgtccggg cgaaggcctc gagcgcgtgc tcgacaagct
      361 ggagggtcag accgcccgcc cgctcgccgc ggaacggcag gtcgacgacc gcgtacggcc
      421 gcccaccgac atcgatgatc gccgtagcga ccgcctcgtc catcggcacg ctcgcgtcgc
      481 cgaagcggac gatcccggcg cgatcgccga gagcttccgc gatggcggaa cccagcacga
      541 gcgcgacgtc ctcgaccgtg tgatggtcgt cgacctcgag gtcgccggtc gcccggatct
      601 cgagatcgag caggccgtga tgggccagcg atccgaggag gtggtcgtag aagccgaccc
      661 cggtccggat gtcggccgtg cccgaaccgt cgaggtcgac cgtgacccgg acgtcggtct
      721 cgcgggtgac gcgccggacc gtgacccggc gaccctcgcg ggccacgacc tcgagggatc
      781 cgagcggggt cgtcatcggg tcgtgcctgc tccctcgatc tcgccggcgg cggcgatcaa
      841 gcggtcgttg ccggcgcggt cgcggatcgt caaccgcagg cagtgggcga gcggatggcc
      901 ggcgccgaac gtccggggca cgagcccccg gccgaggagt ccggtcgctg tggctgccgc
      961 acgatcggtc gtgccgaagt cgacgaggag gaagttcgtg acggtcggtc cgacggccca
     1021 cccggcatcg cgcagggcgc cggagaggcg gtcgcgctcg gcgtcgaccc gttcgacatt
     1081 cgcccgcatc ccgcgcgggt cggcgagcgc ctcggtgacg accgtgacgc tgatcgtgga
     1141 gacggagccc ggcggccggt agggcgcgat cctggcgatc gtctcgggcc gggcaatggc
     1201 gaacccgacc cggaggccgg ccagcgcata ggccttgctc gccgtgcgga cgacgacgag
     1261 gttgggatgg tcgagccgga ggtcgagcag ggaggctccg acgaactccg cgtaggcctc
     1321 gtcgagcacc acgatcgccg gcgctcggcc ggcgcgttgc gcatcggcgg cgatcccgtc
     1381 gaggagcgtc gcgatcgcgc ctgcgggctc ggggagcccg gtcgggttgt tcgggctgca
     1441 cagccagacg agctccgcgt cgacggcggc ctcgcgggtc gggccgggat cgacggcgta
     1501 gcccgcgccg gcaccgagtc gcgggacgag gacggcggtt gcgccccgct gctcggtcac
     1561 gatccggtac atcgcatacg tcggcgccgg cacgaccgcg aggctgcccg gcgccaggaa
     1621 tgccttgccg gcgaggtcga ggatctcgtc cgcaccggca ccgaccagca gctcatcggt
     1681 gccgacgcca tagacgccgg ccgcggtctc gaccaggcga cgatagtcgg acggcgggta
     1741 ctcggacagg ggtgcatcga acgtgcccgc ggccaggatt ccggccacga agtcgggcgg
     1801 cgccggcgac gtgttcaggt cgaaccgcag gatctgctcg atcgggatgc catagcgagc
     1861 tgcaacctct gcgtcggttg cctcccagct gtagctcgcg gggtgggtcg gcatgccgaa
     1921 ggtcaccgcc gtcaccgggg acgggctcat cggtcgactc cggtggcggc gtcggagcgg
     1981 tcgcggaagc gcgcctcgac ggcgttgcgg tgggcggtca ggccttcggc tgtggcaagg
     2041 cggccgaccg tctcgcggat ggcggcaagg ccgcgctcgt cgacgcgctg gatctgcgtc
     2101 cagcgaccga acgcctcgac cgccagcggg tcggcggatc gggcaaggcc gcccgtcgga
     2161 aggacgtggt tcgccccggt cgcgtagtcg ccggcggatt cgggcgccca cggcccgacg
     2221 aagatcgaac cggcgtggcg gatccgggcg gccgcggcgt cggggtcgcg gacatcgatc
     2281 gagagatgct cgggcgcgta ggcgtccacg aaggcgatcg ccgcgtcgag atccggggcc
     2341 agggcgatcc tgccgtggct ggcgagggcg cgggcaagga tgtcgcgacg ctcgaggcgg
     2401 ggaaggaggg cggcgacctc gcgctcgacg gcgtcggcga cggcgaggct ggtcgtgacg
     2461 aggatcgcgg gcgagtcggg accgtgttcg gcctgggtga cgagatcggc ggcgacgagg
     2521 acgggatcgg cggtgtcgtc ggcgaggacc atcccctcgg acggccccgc cggcaggtcg
     2581 atcccgacgg ccccggcgac ctcgagcttg gccgccgtga cccacgcgtt gccgggtccg
     2641 acgatccggt cgaccgcggc caggggcggc gtcgtgtccc gcaggccgaa cgccagggcg
     2701 ccgatcgcct gggcgccgcc cgcgacgagc agggtgtcga cgtccagcag gccggcgacc
     2761 ccgaggagga cagggtggac ggcgcccgag gcgtcggcag gggaggcgac gacgatcccc
     2821 gcgaccccgg cgacccgcgc gggcacgacc gtcatgatca gcgacgacgg gtacggcgcg
     2881 gagccgcccg ggacatagca gccgacccgg tcgagcggca gccagcgccg ctcgatctcg
     2941 atccccggga cgatcccggt ccttgtcgtc gcgggtcgct gggtcgctgc gaatttcgcc
     3001 acgtgctcga tcgcctggtc gagggcccgc cgatccgggg cagcgaggcg gtcccgcgcg
     3061 gcgcgcagct cgccccgatc gatgacgagg cggccatcgg ccagcgcgcc gccgaaccgc
     3121 gcgccggcct cgacgacggc ggcgccagca cgggtccgga cgtcggccag gatcgaccgg
     3181 gcgccgctcc ggaccgcgtc atcgggaacg gcgccgcggc ggcacagcgc tgcccgttcg
     3241 cggatgtccg ccgcagccgt cgaggacagg tcgagacggc gcagacgaac ggtggcggcg
     3301 agaggatcga gcgaggcgga cgtcgtcacg gaacgatctt ctcgatcggc aggacgagaa
     3361 tccccgacgc tcccgccgcc ttcaggcggg gcaacaggct ccagacatcg tcggccccga
     3421 cgacggcgtg aatcgcaatc atccctgcgt gggccagcgg gatgaccgac ggcgactcga
     3481 ggccgggcag gagcgcctcg agatcgcgca gccggacggc cggcgcgttc atcatcaggt
     3541 acttccgacc gcgggcggcg atgaccgcac tgagcatcgt gtcgatggcg tcgatctggg
     3601 tcgcacgctg gacgacggcc gtggggttgg cgacgaggat cgcctcggag gcgagcacgt
     3661 cggcgatcgg ccggaggccg ttcatcacca gggtcgagcc ggtcgagacg agatcgacga
     3721 tggcctcggc cagcccgagc cgcggcgcaa cctcgacggc acccgagatg gggatgacgt
     3781 cgacggcgat gccggcggcg gcgaagaacc gacgggcggt gttcggatgg gcggtcgcga
     3841 cgcgcagtcc ggcgaggtcg tcgagctcgc gcagggcgct gtcggcgggg accgcggccg
     3901 ccaggcggca ctggccgtag ccgagcgacc ggagtcgcgg gagctcggca ccggtctcgg
     3961 ccaggaggtc ctcgccggtg accccgaggt cggccacgcc gtcaccgacg aactcgatga
     4021 cgtcgttggt ccggacgaac aggatgtcga ggtcgaaatt gcggacccgt gcgacgaggc
     4081 tccgttcgtg ctcctcgaac acgaggccgg cgtcacgcag cagttcgacg gtcggttcga
     4141 cgagtcgtcc cttgttcggg atggccagcc ggagtcgctc gcgtccttcg agggcactca
     4201 ccgttcggcc cgggtggggt aggggatccc ctcctcgcga gccgccgcga ggcgctcgag
     4261 ggccgcgcga tagcccccct cgccgtggtt gagccaggag gcgatccggg tgatcgtcgc
     4321 ggtgctggca ccggtctccc gcgagatcgc ggcgtagtgc tcgccggcat cgaggcggcg
     4381 gacgaccgcc cagcgctggg cgaggtcgcg gagctcgccg agggtgcaca ggtcgcgcag
     4441 gaacgacgcg gcctcgtcag cagtctcgag gcggagcacg gcggagatca actcgtcgac
     4501 gtcggaggaa cgccagtcat ccatcggttc gccgggtcct tccgggccgg acgcccgtgt
     4561 ggctgattgg ctcgcaccgt tttagcatgc taccatgcta gcgtgtcatc acagttcccg
     4621 tcaacgttca ccatccttcc ggcgatcgac ctccgcggcg gtcgggtcgt ccgcctgaaa
     4681 cagggagatt tcgctcggga gcaggtctac gcggacgatc cgttggcggt ggcgcgtgag
     4741 ttcgctgccg ccggtgcggg gtggatccat attgtcgacc tggacggagc cagggcgggg
     4801 gagcgccgcg agacgtccgt catcagcgca ctcgtcgccc tgttgcgcgg cctcggttcg
     4861 cgggtccagg tcgccggagg cctgcggacg gcacaggcgg tcgccgacgt cctcgaggcc
     4921 ggcaccgaac gggtcgtgat cggcacggcc gcgctgcgcg acccggcgtt cgtcgaggcg
     4981 gcctgcgcgc gccacggtcc gccacggatc gcggtcgccc tcgacgtccg cgatggcgat
     5041 gcaatcggcg atggctgggt tgcgggcgcc gcgggcgtcc cggttgttga cgcgctgtcg
     5101 cgcctggcgg cggtcggtgt cacgacgttc gcggtcaccg ccatcgagcg cgacggtctg
     5161 ctcgatggac cggacctcgc cctgctcgag cgctgtgtcc gttcgaccga cgctgcggtc
     5221 cttgcctcgg gcgggatccg atccgtcgac gatctcgagg ccgtcgggcg catcggctgc
     5281 agcggagcca tcgtgggccg ggccatctac gacgggagcc tcgatctcgc cgcggccatc
     5341 agcgcgatcg gcgcaacggc gatctgagcc gggcggaggg cgggcgacgc gattgcccgg
     5401 tcgtgcgggc cccaccgtac ccgttcagcg ggatcgggca cgcccggacg gatcagtgcc
     5461 cgccgtgcgc gcctgtggcc tcagctccct tgcgatgcgc gtcgatcacc ttctcggcga
     5521 cgtggccggg gacttcctcg tagtggtcga ccaccgccgt gaacgtgccc cggccgtggg
     5581 tcagcgagcg cagctccgtc gcgtaggaaa agagctccga ctgcgggacc tgggccgaca
     5641 cgacctgctg gccgtcaccg agcgtgtcca tcccgaggac ccggcc
//
LOCUS       DATAIW010000033         8673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_150080, whole genome shotgun sequence.
ACCESSION   DATAIW010000033 DATAIW010000000
VERSION     DATAIW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150080"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..724)
                     /locus_tag="VHM48_01195"
     CDS             complement(32..724)
                     /locus_tag="VHM48_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX2754043.1"
                     /translation="MADYEEIDLSALSDDELVEQMHNDLYDGMRDEIIEGTNLLLGRG
                     FAATRVLDDALVAGMKIVGIDFRDGILFVPEVLLAANAMKAGMAILRPLLAETGAEPV
                     GKVVIGTVKGDIHDIGKNLVGMMLEGAGFEVVDLGINTDAEKFIAALELHKPDILGMS
                     ALLTTTMPYMKVVIQTLKDRGMRDDYIVLVGGAPLNEEFGLAVGADAYCRDAGVAAET
                     ARRLIDERRAAA"
     gene            856..1611
                     /locus_tag="VHM48_01200"
     CDS             856..1611
                     /locus_tag="VHM48_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2754044.1"
                     /translation="MSRVQSIERAFAVLGALTAGPIGVTEVADRVSLPKSTAARMLVS
                     LAREGAVEQIPGDTRYRLGPRIESLATGLVATRSVVAIARPHLAELAASIGEAAGLSI
                     PEGDTMHYVEQVDSDHPVGVRDWTGSRIAMHAVSSGLAVLALWPRARIEAYLGRPMER
                     FTDRTMTDPVELRTRLQRIQLDGRAWTNGEYAEGIASVAAAIADDSGEVVAAVHVHGP
                     SYRFPPPGREEALGLLVATTATRIARTLRQSAG"
     gene            1810..2517
                     /locus_tag="VHM48_01205"
     CDS             1810..2517
                     /locus_tag="VHM48_01205"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2754045.1"
                     /translation="MKTRPAAPSTSPVEAGDVSSGPDRAGRRASRPRPTAESPSRIAR
                     PVGRPRSGTADRAILDAAMALLSEVGLAGLTMSAVVSRSQVARATVYRRYPTREALLS
                     AALAQVKGREPFELSGDIAADIETGAAWAAAVLAEAPFQRFLPLFVTESLRGPAAARS
                     LIARVAPNHGGIAQEYGEAAAGAGLRTDIDPALVADIVQGTMMMRLLSTGVPPDATAV
                     QQLVEVLLRGLREPAAD"
     gene            complement(2557..4245)
                     /gene="betA"
                     /locus_tag="VHM48_01210"
     CDS             complement(2557..4245)
                     /gene="betA"
                     /locus_tag="VHM48_01210"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX2754046.1"
                     /translation="MTDRIWDYVIVGGGSAGSVLANRLSADPATRVLVLEAGRRDWRV
                     DAFIHMPAALTFPIGSRFYDWMYESEPEPFMGGRRVSHARGRILGGSSSINGMIFQRG
                     NPLDYERWAADPGMETWDYAHCLPYFRRMEDCTAAAADDPWRGHGGPLGLERGPATSP
                     LFRAWFEAAQEAGYPLTSDVNGYRQEGIAPFDRNIRRGRRISAARAYLHPVMGRRTNL
                     KVRTSTLVTRIRVDARRAVGVEIERHGGATETIRAGEVILCGGAFNTPQLLQLSGVGG
                     GAELERLGIPVVADVPGVGEHLQDHLEVYIQHGSSQPVSEQPSATQKWRRPFIGAQWL
                     FLRSGPGATNHFEAGGFVRSNDDVAYPNLMFHFLPLAIRYDGTPAPSKHGYQVHVGPM
                     YSDARGSVKITSTDPHRHPALRFNYLSTAQDRREWVEAIRTARRILGQPAFAPYSTGE
                     LSPGAGVETDEEILAWVARDAETALHPSCTARMGVDRESVVDPLTMGVHGLVGLRAVD
                     ASTMPYVTNGNIYAPVMMLAEKAADLIAGNTPLPAQATPFYRHGASPLEEPPRG"
     gene            complement(4294..4818)
                     /locus_tag="VHM48_01215"
     CDS             complement(4294..4818)
                     /locus_tag="VHM48_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2754047.1"
                     /translation="MTQQGAETATPEHMHSLAPGPIVLGTEFGSVSSAAERVAIRLAK
                     RAGVPLLIVHGIDPGRLRLPGGRFSQRVDQARAARQVDAAALMERVRSAGVEPQLLIW
                     DGDPATCVVEAATAEGASRIVVGSHGRGRLGRAIIGSVSATVAGQAECPVDIVRGDGS
                     SEDIVTVSPTSRRS"
     gene            4923..6146
                     /locus_tag="VHM48_01220"
     CDS             4923..6146
                     /locus_tag="VHM48_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2754048.1"
                     /translation="MSDVLDSSSIRTPAASEFGELLPGAPALAAVDEAARAISSVLGI
                     EEVLQLIVDRVRDLVDARYAALGIAGVDGRIERFITAGVDPHERLAIGALPSGRGLLG
                     LIIREGQSFRIPSIADHPDSVGFPRNHPQMTSFLGVPVMAHGSSVGNLYLTDKREAAE
                     FSLGDLRLVELFASHAGIAIDNARLHAQAGRLAVAEERDRIGRDLHDGIIQSLYAVGL
                     SLEDVPDLMAEAPDEASERVDIAIESINLAIRDIRNFIYGLRPEAVDGTQVIAGLAAL
                     AEEVRHGGLVDVVADLDGSDDPGLDADAGAELLNLVREALSNAVRHGHAVQISIGMST
                     NDVASMLEIADDGVGFDPAQVVGAGHHGLANMRARAAAIGGQLEIRSAPGEGTRVIVE
                     LPRPTSTTEDQDSQR"
     gene            6143..6832
                     /locus_tag="VHM48_01225"
     CDS             6143..6832
                     /locus_tag="VHM48_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2754049.1"
                     /translation="MTEERPIPARGSAGRPFRLLVVDDHEVVRQGLVALLDRRPNFQV
                     VAEAGTVEEALAQARLHQPDIVIMDVRLPDGSGVEACREIRADLPDTRVVMLTSFPDD
                     EAVLSAIVAGAAGYLLKQIRARDLVSALEAVGRGESLLDPAVTERVLARVRQIASGEI
                     HDELAGLTPQERKILLLVAEGKTNKAIASEIFLSDKTVKNYVSSILAKLNLERRAQAA
                     AFVARHRLDRG"
     gene            complement(7066..8061)
                     /locus_tag="VHM48_01230"
     CDS             complement(7066..8061)
                     /locus_tag="VHM48_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2754050.1"
                     /translation="MTAPILALVTITDGVPERASLEALSLARRLAAETGAVLEAVLLA
                     DIAAARPAATTLAGSGVTTVIVIEHPNLTADHPDGWAAAVAQIVEARRPAAVVGAGTE
                     RGNELLARVAARMSLPMAANCLSVEPGERYRSTRQRWAGSLLEDCWLDADIRLITVAP
                     NSVAIGEPDASLAPAIEAVAVTLSDRDLAVHVTSREAPERGRLSLADAKVVVGGGRGV
                     GSGEGFADLEELAGLLGGAVGGSRVVTSAGWRPHADQIGQTGLRIAPDVYIACGISGA
                     IQHVVGCKAAKHILAINTDPDSPIMSVADYAVIGDLHTILPAISAEIRRRSAARA"
     gene            complement(8058..>8673)
                     /locus_tag="VHM48_01235"
     CDS             complement(8058..>8673)
                     /locus_tag="VHM48_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754051.1"
                     /translation="GGASAVLTLGPAAAEEQLRDAMAIGVDRAILLETDGRDWDPIAT
                     AGAIVEAIRAKEAADGPFDLILFGNESADSGGFQVGIRVAASLDRPCVTGVKSLQPGD
                     GRIVARREAPSGGWESFEVPLPAVVSVKEGINLPRYPSIPGRLRARKKEIERSSPAWH
                     AGGLEKIVLRLPKEELTAAEVIGRGPDAAPAVVDLLTRIGVLDA"
BASE COUNT         1232 a         3097 c         3079 g         1265 t
ORIGIN      
        1 cgcggaccgg gcggctcggc gaggcccgat ctcaggccgc ggcacgtcgt tcgtcgatca
       61 ggcgacgggc ggtctcggca gccacaccgg cgtcacgaca gtaggcgtcg gcgccgacgg
      121 ccagcccgaa ctcctcgttg agaggcgcgc cgccgacgag gacgatgtag tcgtcgcgca
      181 tcccgcgatc cttgagggtc tggatcacga ccttcatgta cggcatggtc gtcgtcagga
      241 gggcgctcat cccgaggatg tcgggcttgt gcagctcgag ggcggcgatg aacttctcgg
      301 cgtcggtgtt gatcccgagg tccacgacct cgaagccggc accctcgagc atcatcccga
      361 cgaggttctt gccgatgtcg tggatgtcgc ccttgaccgt cccgatgacg accttgccga
      421 ccggctcggc gcctgtctcg gcgaggaggg gccgcaggat cgccatcccg gccttcatcg
      481 cgttggccgc gagcaggacc tcgggaacga acaggatccc atcgcggaag tcgatgccga
      541 cgatcttcat cccggcgacg agcgcgtcat cgagaaccct ggtggcggcg aagccgcggc
      601 cgaggaggag gttcgtgccc tcgatgatct cgtcgcgcat gccgtcgtac aggtcgttgt
      661 gcatctgctc gacgagctcg tcgtcgctca gcgcggatag gtcgatctct tcgtagtcgg
      721 ccacgccggt gctccctgac cgccggctcc gcccgccgac tgaggaccgc catcatctca
      781 cgttgcgggt tccacattgt ggaacggtgc cgtatgatgg aacgcggaac cagaatctag
      841 ccgacgggga agcgcgtgtc cagagtccag tcgatcgaac gtgccttcgc ggtgctcggg
      901 gcgttgaccg ctggccccat cggggtcacc gaggtcgccg accgtgtctc cctgccgaaa
      961 tcgacggccg cccggatgct ggtctcgctg gcgcgggaag gtgccgtcga gcagatcccg
     1021 ggcgacacgc gctatcggct cggaccgcgg atcgagagcc ttgccacggg tctggtcgcg
     1081 acccgcagcg tcgtcgcgat cgcccggccg cacctggccg aactcgcagc gtcgatcggc
     1141 gaggccgccg ggctgtcgat ccccgagggc gacacgatgc actacgtcga acaggtcgac
     1201 agcgaccatc cggtcggcgt ccgcgactgg accggctcgc ggatcgcgat gcatgccgtg
     1261 tcgtcggggc tggcggtcct cgccctgtgg ccgcgcgcgc ggatcgaggc ctacctgggc
     1321 aggccgatgg agcgattcac cgaccggacg atgaccgacc cggtggagct ccggacccgc
     1381 ctgcagcgga tccagctcga cggtcgtgcc tggaccaacg gtgagtacgc cgagggaatc
     1441 gcatcggtgg ccgccgcgat cgccgacgac agtggtgagg tcgtcgcggc cgtccacgtc
     1501 cacggtccgt cgtaccggtt cccgccaccg ggtcgcgaag aggcgctcgg cctgctcgtc
     1561 gccacgaccg cgacgcggat cgcgcgaacg ctccgccaga gcgccggctg agacaacacc
     1621 ggaccggatc gccgggggcc ggctctcgcc gacgccgccc aacgtcggga ggcgtcgatc
     1681 tggcaggccg gtgggtgaag ggaccccctg aggcggctct cgccgggctg acgaagccgc
     1741 gacgtcgccc agaccctgaa ggtttcgata cgcagtgtct agtatcacaa ttgctggtag
     1801 cctgtcgtgg tgaagacacg tcccgccgct cccagcacgt cgccggttga ggcaggcgac
     1861 gtctcgtccg gtccggatcg ggccggccgg cgcgcgtcgc gcccgcggcc gaccgccgaa
     1921 tcgcccagcc ggatcgcgcg accggtcggc cgtccccgca gcggaaccgc cgaccgggcg
     1981 atcctcgatg ccgcgatggc tctcctctcc gaggtcggcc ttgccggcct gacgatgagc
     2041 gccgtcgtca gccggtcgca ggtcgccagg gcgacggtct atcgccgcta tccgacgcgg
     2101 gaggcgctcc tgtccgccgc tctcgcccag gtcaagggcc gcgagccgtt cgagctgtcc
     2161 ggcgacatcg cagccgatat cgaaacgggg gcggcgtggg cagccgccgt cctcgccgag
     2221 gcgccgttcc agcggttcct gcccctgttc gtgacggagt ccctgcgcgg accggcggcc
     2281 gcacgcagcc tgatcgcccg ggtcgccccg aaccacgggg gcatcgccca ggagtacggc
     2341 gaggctgccg ccggcgccgg gctgcgaacg gacatcgatc cggccctcgt cgccgacatc
     2401 gtccagggaa cgatgatgat gcggttgctg agcaccggcg tgccgcccga cgcgacggcg
     2461 gtccagcagc tcgtcgaggt cctccttcgc ggcctgcgcg agcccgcggc cgactgaggg
     2521 gccgacgccc ctcgcgaggg atccacgtcc gggcggtcag cctcgcggcg gctcctcgag
     2581 cggcgacgct ccgtgtcgat agaacggcgt cgcctgcgcc ggcagcggcg tgttgcccgc
     2641 gatcaggtct gccgccttct cggcaagcat catcaccggc gcgtagatgt tgccgttcgt
     2701 gacgtacggc atcgtgctcg cgtcgaccgc ccgcaggccg acgaggccgt ggacgcccat
     2761 cgtcaacggg tccacgacgg attcgcgatc gacccccatc cgcgccgtgc aggacggatg
     2821 gagcgcggtc tccgcatccc tggcgaccca ggcgaggatc tcctcgtcgg tctcgacgcc
     2881 ggcgcccggt gacagctcgc cggtcgagta cggcgcgaat gcgggctggc ccaggatccg
     2941 acgtgccgtg cggatcgcct cgacccattc gcggcggtcc tgggcggtcg acaggtagtt
     3001 gaaccggagc gccggatggc ggtgcgggtc ggtcgacgtg atcttcacgc tgccgcgggc
     3061 atcggagtac atcggcccga cgtggacctg gtagccgtgc ttgctcgggg cgggcgtccc
     3121 gtcgtagcga atcgcgagcg gcaggaagtg gaacatcagg ttcgggtagg cgacatcgtc
     3181 gttggagcgg acgaagccgc cggcctcgaa gtggttcgtc gcgccgggtc cgctgcgcag
     3241 gaacagccac tgggcgccga tgaacggccg ccgccacttc tgcgtcgcgg acggttgctc
     3301 cgagaccggc tggctggagc cgtgctggat gtagacctcg aggtggtcct ggaggtgctc
     3361 gccgactccc ggcacgtcgg cgacgacggg gatcccgagc cgttcgagct ccgcgccgcc
     3421 gccgacaccg gacagctgca gcagctgggg cgtgttgaag gcgccgccac agaggatgac
     3481 ctcgcctgcc cggatcgtct cggtcgcgcc gccgtgtcgc tcgatctcga ccccgaccgc
     3541 gcgccgcgcg tcgacacgga tccgggtcac cagggtcgac gtccggacct tcaagttcgt
     3601 ccgccgaccc atgaccgggt ggagataagc ccgcgccgca ctgatccgcc ggcctcgccg
     3661 gatgttccgg tcgaacggtg cgatgccctc ctggcggtag ccgttgacgt cgctcgtgag
     3721 cgggtatccg gcctcctggg ccgcctcgaa ccacgcccgg aagagcgggc tggtcgccgg
     3781 gccgcgttcc aggccgagcg ggccgccgtg gccgcgccac gggtcgtcgg cggctgcggc
     3841 ggtgcagtcc tccatccgcc ggaaataggg caggcagtgg gcgtagtccc aggtctccat
     3901 gcccgggtcc gccgcccagc gctcgtagtc gagcgggttg ccgcgctgga agatcatccc
     3961 gttgatcgag ctcgagccgc cgaggatcct gccccgcgcg tgggacaccc ggcggccgcc
     4021 catgaacggc tcgggctccg attcgtacat ccagtcgtag aaacggctgc cgatgggaaa
     4081 ggtcagcgca gccggcatgt ggatgaaggc atcgacgcgc cagtcgcgcc gtccggcttc
     4141 gaggaccagg acgcgggtcg ctgggtccgc gctcaagcgg ttggcaagga cactgcccgc
     4201 gctcccgccg ccgacgatca cgtaatccca gattcggtcg gtcatcggcg ccgatcctag
     4261 cagcgtgatg ggctcgaacg gccagggcgg ctgtcaggaa cggcggctcg tcgggctgac
     4321 ggtgacgatg tcctcgctgg agccgtcgcc gcggacgatg tcgaccgggc attcggcctg
     4381 gccggcgacc gtcgccgaga cgctgccgat gatcgcccgg ccgaggcgtc cgcggccgtg
     4441 cgaccccacg acgatgcgcg acgcgccctc ggcggtggcc gcctcgacga cgcaggtcgc
     4501 cggatcgccg tcccagatga ggagctgcgg ctcaacaccg gccgagcgga cgcgctccat
     4561 gagggcggct gcgtcgacct gacgagcggc gcgcgcctgg tcgacacgct gcgagaaccg
     4621 tccacccggc aggcggagac gacccggatc gatcccgtgg acgatcagca gcgggacgcc
     4681 ggctcgcttg gccaggcgga tcgcgacccg ctctgcggcg gacgagaccg acccgaattc
     4741 ggtgcccagg acgatcggac ccggcgccag ggaatgcatg tgctcggggg tggccgtctc
     4801 ggccccttgc tgggtcatgg cggccagagt ggtcgtcgcg gggcacacca gacaggtgcc
     4861 gtgtggcccg cgacccgggg tccggtggtc acgaacggcc gggaaggcgt tacgctcgcg
     4921 ccatgtcaga tgtcctggac tcgtcctcga tccggacgcc cgcggcgagc gaattcggcg
     4981 aattgctccc gggcgccccc gcgctcgcgg cggtcgacga ggcggcgcgt gccatctcga
     5041 gcgtcctcgg gatcgaggag gtcctccagc tgatcgtgga ccgggtccgc gacctcgtcg
     5101 acgcccgcta tgccgccctc gggatcgccg gtgtcgacgg gcggatcgag cgcttcatca
     5161 ccgccggcgt cgacccgcac gagcggctgg cgatcggggc gttgccgagc ggacgcggac
     5221 tcctcggcct gatcatccgc gagggtcagt cgttccggat cccgtcgatc gccgatcatc
     5281 ccgactccgt gggattcccg cgcaaccacc ctcagatgac gtcgttcctc ggcgtgccgg
     5341 tgatggccca cggttcgtcg gtcggcaacc tctacctcac cgacaagcgc gaggccgccg
     5401 agttctctct cggcgacctg cgcctcgtcg agctgttcgc cagccatgcc ggcatcgcga
     5461 tcgacaacgc ccgcctccat gcccaggccg ggcgcctggc cgtcgcggag gagcgcgacc
     5521 ggatcggccg cgacctgcat gacggcatca tccagtcgct ctacgcggtc gggctctccc
     5581 tcgaggacgt cccggacctc atggccgagg cacccgacga agcgtccgag cgggtcgata
     5641 tcgcgatcga gtcgatcaat ctcgcgatcc gcgacatccg caacttcatc tacgggctgc
     5701 gtccggaggc cgtcgacgga acgcaggtca tcgccggcct ggctgccctc gcggaggagg
     5761 tccgccacgg cggtctcgtc gacgtggtcg ccgacctcga cggctccgac gaccccggcc
     5821 tcgacgcgga tgcgggcgcc gagctcctga atctcgtccg cgaggcgctc agcaacgcgg
     5881 tccgccacgg gcatgcggtg cagatctcga tcggcatgtc gacgaacgac gtggcgagca
     5941 tgctcgagat cgctgatgat ggcgtcggct tcgacccggc gcaggtcgtc ggggcagggc
     6001 atcatggtct cgccaacatg cgtgcccgcg cagcggccat cggtgggcaa ctcgagatcc
     6061 ggagcgcgcc gggggagggc acgcgggtga tcgtggagct gccgcgaccg acatccacca
     6121 cggaggatca ggacagccag cgatgaccga ggaacgaccg attcccgccc gcggttcggc
     6181 cggccggccc ttccggctgc tggtcgtcga cgaccacgag gtcgttcgcc agggcctcgt
     6241 ggcgctgctc gaccggcgtc ccaacttcca ggtcgtcgcc gaggccggca cggtcgagga
     6301 ggcccttgcc caggcacgtc tccatcagcc cgacatcgtg atcatggacg tccgcctgcc
     6361 cgacggctcg ggtgtcgagg cctgccgcga gatccgggca gacctccccg ataccagggt
     6421 cgtgatgctt acctcgtttc ccgacgacga ggcggtcctg tcggcgatcg tggcgggcgc
     6481 cgcggggtac ctgctcaagc agatccgtgc ccgcgacctg gtgtcggcac tggaggcggt
     6541 cggccggggc gagtcgctcc tcgatccggc ggtcaccgag cgggtcctcg ctcgggtccg
     6601 ccagatcgcc agcggcgaga tccacgacga gctggccggg ctgaccccgc aggagcggaa
     6661 gatcctgctc ctcgtcgcag agggcaagac gaacaaggcg atcgcatcgg agatcttcct
     6721 ctccgacaag acggtcaaga actacgtgtc gtcgatcctg gcgaagctca atctcgagcg
     6781 gcgcgcccag gccgcggcgt tcgtcgcccg ccaccggctc gaccggggct gagggtcccg
     6841 cgcgggcgcg ctgccgcacg tcgccctcac ggccaccggc ctcgcccgcg ccaccggcct
     6901 cgcccgcgct gggcctgcga atagccgaat actgcggatc tcgaatcatt cggctattcg
     6961 aaacgggctg ccgggcgcgc ccgaaccggc gggcgggcgg atcgaaaacg agctcgcgag
     7021 acacgcgttc cgggccgggc attcgcggga cgcggcgccc gatcctcagg cccgggccgc
     7081 gctccggcgc cggatctcgg ccgagatcgc cggcaggatc gtgtggaggt ccccgatcac
     7141 cgcatagtcg gcgacgctca tgatcggcga gtcgggatcc gtgttgatcg caaggatgtg
     7201 cttggcagcc ttgcagccga cgacgtgctg gatcgccccg ctgatcccgc aggcgatgta
     7261 gacgtccgga gcgatccgca atccggtctg gccgatctgg tcggcgtgcg gccgccagcc
     7321 cgcgctcgtc acgactcgcg agccgccgac ggccccgccg agcaaccccg cgagctcctc
     7381 gaggtcggcg aagccctcac cactgcccac tccgcggccg ccgccgacga cgaccttcgc
     7441 atcggccagg gagaggcggc cgcgctcggg cgcctcgcgg ctcgtcacgt ggacggcgag
     7501 gtcgcggtcg gacagcgtca cggcgacggc ctcgatcgcc ggcgcgaggc tggcgtcggg
     7561 ctcgccgatc gcgaccgagt tcggggcgac tgtgatcagc cggatgtcgg catcgagcca
     7621 gcagtcctcg aggaggctgc ccgcccatcg ctggcgggtc gagcggtacc gttcgcccgg
     7681 ctccaccgag aggcagttgg cggccatcgg caggctcatc cgggcggcga cccgggcgag
     7741 gagctcatta ccgcgctcgg tcccggcgcc gacgacggcc gccggccggc gcgcctcgac
     7801 gatctgggcc accgcggccg cccacccgtc gggatgatcc gcggtcaaat tcgggtgctc
     7861 gatgacgatc accgtcgtga cgccggaacc ggccaatgtc gtggccgcag gccgggccgc
     7921 agcgatgtcg gcgaggagga cggcctcgag gacggcgccc gtctcggcgg caaggcgacg
     7981 ggccaggctc aatgcctcca gcgaggctcg ctcgggaacg ccgtccgtga tcgtgacgag
     8041 ggcgaggatc ggtgcggtca tgcgtcgagc accccgatcc gggtcagcag gtcgacgacg
     8101 gccggcgccg cgtccgggcc acgaccgatc acctcggccg cggtcagctc ctccttcggc
     8161 aggcggagga cgatcttctc gaggccgccc gcgtgccagg cgggcgacga ccgctcgatc
     8221 tccttcttcc tggcgcgcag ccggcccggg atcgacgggt aacgcggcag gttgatccct
     8281 tctttgaccg agacgaccgc gggcaggggg acctcgaagg actcccagcc gccgcttggc
     8341 gcctcccgcc gcgcgacgat ccggccgtcg ccgggctgga gcgacttcac ccctgtcacg
     8401 caaggccgat cgagcgaggc ggcaacccgg atcccgacct ggaacccacc gctgtcggcc
     8461 gattcgttcc cgaagaggat caggtcgaac ggtccgtcgg cggcctcctt ggcccggatc
     8521 gcctcgacga tcgcgccggc cgtcgcgatc gggtcccagt cccgcccgtc ggtctcgagc
     8581 aggatcgccc gatcgacgcc gatcgccatc gcgtcgcgga gctgctcctc ggccgcggcc
     8641 ggaccgagcg tcagcacggc gctcgcgcca ccg
//
LOCUS       DATAIW010000034         7502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_60900, whole genome shotgun sequence.
ACCESSION   DATAIW010000034 DATAIW010000000
VERSION     DATAIW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60900"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHM48_01240"
     CDS             <1..265
                     /locus_tag="VHM48_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2754052.1"
                     /translation="VDIAAAQLDGEVRVSVSDTGPGIAPEDQERIFEEFQQAAAGKEQ
                     REGTGLGLALSKRLVELHGGHMWVDSELGKGSTFVFTLPAQPA"
     gene            268..648
                     /locus_tag="VHM48_01245"
     CDS             268..648
                     /locus_tag="VHM48_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2754053.1"
                     /translation="MAGEQILVVEDNEKNMKLLRDVLQATGYRTLEASTGRQALTLAT
                     EHRPALVLMDIRLPDMDGVEMLSRLRMDERTASIPVLAVTAQAMKGDRERFKEAGFDG
                     YLSKPVDIDELLTAVEQHCGGRGG"
     gene            complement(707..3823)
                     /locus_tag="VHM48_01250"
     CDS             complement(707..3823)
                     /locus_tag="VHM48_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754054.1"
                     /translation="MNALDLEMDRRRFLALLGSLTAAGALAGCGSQDVSPRPAATAAS
                     APPSHGPVGPYDVLRRLQAIIRQSPDHLGSLAAAAVATGDPAAIVRFVQEHVAVLPST
                     ARNADPTTDVRWGQRAALRSGAGTLRERADLIVDLLRQAGIEGRVVTVPRPSPDPAGS
                     REVPAFAPDPVAIAALWDPIDPTHPPVGGADDGAGSRADTATERLLAALPIELRNARL
                     VGPALPETIPAVEFQHDGGTRWATGLGREPLLSASPDGLLNASAVVVPRVSVAVSVAL
                     NPPTGATIDRTVLHEVLRGEWTADQVAGRQLVLGFAAPGSAVDTLGQDLAAATIRRPV
                     LRLMAGEPLGDAVPGVTGTYLSTAGGLIEGSPDDPSRVIGPLGPLLAPTAGGAVGSSV
                     AALEGTIVAATFPEVELRLQAWQADGSPVHGLTAGDFRVSEDGAARAVTVIANSRPAD
                     TRVLVVYDTSGSVTDFWSSPARRTAFESTVVKAVGDAAAAHPFVVQVIGVGDVARDDR
                     WAAPDPTALAAAFGAVASNSDVWLTLGRAVPASGAAAVLLVSDNEASDVAADIPAFRR
                     ALRASGVPVACLPVGTVNKATTDLILADGGGPRFSPTAPDLAAMLGAFIGSRVAAAGA
                     TNYRVRYGAPAAGPAQRAVVVAIAGSAVAGLKLTYTVPAEADRAAPPGIAGVYLTIRV
                     GDRESRRRLGGVRVSDRGTPADTADAAAIGDAMAAINGIHTISFEPASSTTAHLLDDV
                     IGAALSAEPIAAAWDRGPAAIAAASAGWRRVPATLAWLADAVTPGSGGSAIADGLRVT
                     ILTERFGPDGLTQLSDVVPALNRAVGSESDPAAAFRSALRATIGASIRESEVFGASAA
                     DLLGSLDLVAIPALESAASVSAWSPEQRARLAPMIEEYADFHRLLPASGDIAAMWVVD
                     PDTGSATAVGADGRGAGSALPDCLTPADTNQASAFISVSIALISAACLAIGAEEPSFG
                     CVGADVFGAVSAGLAVFTAPPDIPGSAFGAFSYGAGLAAANVGSAAGRTIISVLLLIA
                     GLLVGGKC"
     gene            4243..5352
                     /locus_tag="VHM48_01255"
     CDS             4243..5352
                     /locus_tag="VHM48_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX2754055.1"
                     /translation="MSNPSSLPAAQPRRLVILAEGNFGFHHGKTAVGVIRYGPDDVRA
                     VIDSTQAGTNVSAILPGRDIPIVATLEEALATEPRPDTLLIGIAPTGGKLPDSWRTTI
                     LAAIRAGLDVHSGLHTFLADDPEFEAAAHLAGTEILDYRRPPGRMETSVGRRHGPGKR
                     VILTVGTDCAIGKMSVALELRAAARAAGRSTSFVPTGQTGMMIEGWGAAVDRLISDFA
                     QGTVEWLVEEGERRGDWVIVEGQGSLDHPAYSSVTLALIHGATPHAMILVHKAGQTEH
                     DFDHLPDASFPIADLGSFIDLHERVAGLVAPSKVVAIALNTSAIASADDARVEIARVA
                     ADTGLPVDDPVRFGAGPLWTRIEQAVDDLPWVEGR"
     gene            5354..6403
                     /locus_tag="VHM48_01260"
     CDS             5354..6403
                     /locus_tag="VHM48_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX2754056.1"
                     /translation="MALHLSQEILRLGLRDPFRIARSDHDAGSAVTTVVIELRDDRFT
                     GHVGIGEGYPDRFYGDTVETMAAVFPLLLDAVGELEPTRDGLVAAGARMAAAIAHHGG
                     AKCALDIALHDLVGKVKGMSIADLLDLHDPIPPTDFTLGLDEPAVVAERARRAAHFPA
                     LKIKCGGPADLATLEAVRAVYDGPLRVDANTGWQPDDALALLPQLERLGVELIEQPFP
                     ARRLDQLRWLQDRSPLPIVADESAVTVEDLEGLVGVVAGVNVKLAKCGGVGPARAMLA
                     RARELGFRTFLGCMEETSVAIAASAAVASLADWVDLDGNLLLAADPFTGLELDDDCRW
                     RLAGAPGLGIRRQPG"
     gene            6702..7160
                     /locus_tag="VHM48_01265"
     CDS             6702..7160
                     /locus_tag="VHM48_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754057.1"
                     /translation="MNRTNAGERAFAAAPAVETEAIDFVRFCHRRRRVGWPELYDEMC
                     AVAGRGLYRGYGAEELSSIGIGLGLFQMPALAGLVAQVVAEDVERRRRSAEAVRASLA
                     AQAADLVTVAEGGVDCAESGVSPTRRTDPEPADAGPPEHGAIRFAVAAGA"
     gene            complement(7305..>7502)
                     /locus_tag="VHM48_01270"
     CDS             complement(7305..>7502)
                     /locus_tag="VHM48_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754058.1"
                     /translation="RLNRALYTIALVQAWHHPPARAYIARKRSEGMSWKEALRCLKHR
                     IARPMFRLLQGGQARWAALAA"
BASE COUNT         1096 a         2588 c         2774 g         1044 t
ORIGIN      
        1 ggtcgacatc gccgcggcgc agctcgatgg cgaggtgcga gtgtcggtca gcgacaccgg
       61 accgggcatc gctccggagg accaggaacg gatcttcgag gagttccagc aggctgcggc
      121 gggcaaggag cagcgcgagg gcacaggcct cgggctggca ctctcgaagc gcctcgtcga
      181 gctgcacggc gggcacatgt gggtcgacag cgaactcggc aagggcagca cgtttgtgtt
      241 cacactgccg gcgcagccgg cgtagccatg gcgggcgagc agatcctggt ggtcgaagac
      301 aatgagaaga acatgaagct gctcagggac gtgctccagg cgaccggcta tcgcacgctg
      361 gaggcatcga ccggccgaca ggcgctgacg ctggcgaccg agcatcgacc ggcgctcgtg
      421 ttgatggaca ttcggctgcc ggacatggac ggcgtggaga tgctgagccg gctgcggatg
      481 gacgagcgga ccgcatccat accggtgctc gccgtaaccg cacaggcgat gaagggagat
      541 cgcgagcggt tcaaggaggc gggtttcgac ggctatctct cgaagccggt cgacatcgat
      601 gagctcctca cggccgtcga gcagcactgc ggggggcgcg gcgggtgacc gacgagctcg
      661 ttcaagatga gcgcgtcgat cgtgagatcc agcctgcgtt catcgctcag catttccccc
      721 cgacgagcag cccggcgatc agcaacagga ccgagatgat ggtccggccg gcggccgatc
      781 cgacattcgc cgctgcgagg cccgctccgt aggagaacgc gccgaacgcg gaacccggga
      841 tgtccggcgg tgcggtgaag acggccagtc cggccgagac cgcgccgaat acatccgcgc
      901 cgacacagcc gaagctgggc tcctcggcgc cgatggcgag gcaggccgcg gaaatcaggg
      961 cgatcgagac cgagatgaat gccgacgcct ggttcgtgtc ggcgggtgtc aggcagtccg
     1021 ggagcgcgct cccggcgccg cggccgtccg cgccgaccgc cgtcgccgaa cccgtgtccg
     1081 gatcgaccac ccacatggcc gcgatgtcgc cagatgcggg caggaggcgg tggaagtcgg
     1141 cgtactcctc gatcatcggg gcgaggcggg cgcgctgttc cgggctccac gccgacacgg
     1201 aggcagccga ttccaatgcg ggtatggcga caaggtcgag gctgccgagc aagtcggccg
     1261 cgctcgcgcc gaagacctcc gactcgcgga tgctcgcccc gatcgtcgcc cgcaatgctg
     1321 accggaacgc cgctgccggg tccgactcgc tgccgacggc gcggttgaga gccggcacga
     1381 cgtccgacag ctgggtcagg ccgtccggtc cgaaccgctc ggtcaggatc gtcacccgga
     1441 gcccgtccgc gatggcgctc ccgcccgatc caggggtcac cgcgtcggcc agccaggcca
     1501 gtgtcgccgg gacgcgtcgc cagccggcgc tggccgccgc gatcgcggca ggtccacgat
     1561 cccaggcggc cgcgatcggc tcggcgctca gggcggcacc gatgacgtcg tcgaggaggt
     1621 gcgctgtcgt ggacgacgcc ggctcgaagc tgatcgtatg aatcccgttg atggcggcca
     1681 tcgcgtcgcc gatggccgcc gcgtcggcgg tgtcggctgg ggttccccgg tcggagacgc
     1741 gcacgccgcc gagccgccgc cgactctccc ggtcgccgac cctgatggtc aggtagaccc
     1801 cggcgatgcc cggcggggcg gcgcggtcgg cctctgccgg aacggtgtag gtcagcttca
     1861 ggcccgccac ggccgagccc gcgatcgcga cgacgacggc tcgctgggcg ggcccggccg
     1921 ccggcgcccc gtagcgaacc cggtagttgg tcgcgccggc cgcggcgacc cggctgccga
     1981 tgaacgcgcc cagcatggcc gcgagatcag gcgcggtcgg gctgaagcgg ggaccgccgc
     2041 cgtcggccag gatcagatct gtcgtcgcct tgttgaccgt cccgaccggc agacaggcga
     2101 ccggcacgcc cgaggcccgg agcgcgcggc ggaacgcggg aatgtcggcc gcgacgtcgc
     2161 tcgcctcgtt gtcgctgacc agcaggacgg ccgcggcgcc cgacgccggg acggcccgac
     2221 cgagggtcag ccagacgtcc gagttggacg ccacggcgcc gaacgcggcg gcgagggccg
     2281 tcggatccgg cgccgcccat cggtcgtccc gcgcgacgtc gccgacgccg atgacctgga
     2341 cgacgaacgg atgagccgca gcggcgtcgc ccactgcctt gacgacggtg ctctcgaagg
     2401 ccgttcggcg ggccgggctc gaccagaagt cggtgacgct tccgctcgtg tcgtagacga
     2461 cgaggacgcg ggtgtcggcc ggtcgggaat tggcgatcac ggtcacggcc cgggcggcgc
     2521 cgtcctcgct gacccggaag tcaccggcgg tcaggccatg gaccgggctg ccgtccgcct
     2581 gccaggcctg aagacgcagc tcgacctccg ggaaggtggc cgcgacgatg gtgccttcga
     2641 gagctgccac cgacgacccg acagcaccgc cggcggtcgg agcgagcagc ggcccgagcg
     2701 ggccgatgac ccggctcggg tcgtccggcg aaccttcgat caggccaccg gccgtgctga
     2761 ggtaggtgcc ggtgacgcca ggcacggcgt cgccgagtgg ctcgcccgcc atgagccgga
     2821 gcaccggtcg gcggatcgtg gctgctgcga gatcctgtcc gagcgtatcc acggcagacc
     2881 ccggggcggc gaacccgagg accaactggc gaccggcgac ctggtcggcg gtccactcgc
     2941 cacggaggac ttcgtgcagg acggttcggt cgatcgtcgc gccggtgggt gggttgaggg
     3001 cgaccgagac agcgaccgac acccgcggga cgacgacggc cgaggcgttc aggaggccgt
     3061 caggggatgc actcaggagc ggctcgcgcc cgagaccggt cgcccagcga gtcccgccgt
     3121 cgtgctggaa ttcgaccgcg gggatcgttt cgggcagtgc cgggccgacc agccgggcgt
     3181 tccgcagctc gatgggcagg gccgccagca gccgctcggt ggccgtgtcg gcacgcgacc
     3241 cggcaccgtc gtcggccccg ccgacgggcg gatgggtcgg gtcgatcggg tcccacagcg
     3301 ccgcgatcgc gactggatcc ggcgcgaacg ccggcacctc tcgactccca gccggatccg
     3361 gcgagggtcg cgggaccgtc acgacccggc cttcgatccc cgcctggcga aggaggtcga
     3421 cgatcaggtc cgcgcgctcg cgcagcgttc ccgcacccga tcgcagcgcc gcgcgctggc
     3481 cccagcgaac atcggtggtc ggatcggcgt tccgggcggt ggaaggcagg accgcgacgt
     3541 gctcctgaac gaagcgaacg atcgcggccg ggtcaccggt ggcgacggcg gctgcagcaa
     3601 gcgacccgag gtggtccggg ctctggcgga tgatcgcctg gagacgtcgc aggacgtcgt
     3661 acggcccgac cggcccgtgg ctcggcggag cagaggccgc tgtcgctgcc ggcctcgggg
     3721 agacatcctg gctgccgcag ccggcgagcg cgccggctgc cgtcaaggac ccgagcaggg
     3781 ccagaaaccg gcgccgatcc atctcgaggt ccagggcatt catggctggc atccttgcgt
     3841 tcccaacgag ccggtcgggt ccggctattt cgtgaagcag gtccaggtca tcgcgacgtc
     3901 gattggctgc tgcaccttgt tggcggcgat cctgctggct gcgagacgca accacggcgg
     3961 gagcgcttgc cggctttccg gttcgtgacc gtatcacgga cccagggtgg ggcggcccgg
     4021 gctcggatcg ggggaaccct cgctctgatg cttgtccgcg cgggcagcgg gcatgaaacc
     4081 ccggccgacg cgtcgtcatc ggggcgttcc cggtgccccg ccgcgcggca gggccgacca
     4141 tcgccgccat gcccgactcg accgtcgggc gcgcggtcgc cgccgggctg actcggacga
     4201 ggcgatcgat cgggcgatcg ccattctcgt agactcgggc tcgtgagcaa tccatcgagc
     4261 ctgccggcgg cgcagccgcg ccgcctcgtg atcctggccg aaggcaactt cggattccac
     4321 cacggcaaga ccgcggtcgg tgtcatccgc tacggccccg acgatgtccg ggcggtcatc
     4381 gattcgaccc aggccggcac gaacgtgtcg gcgatcctgc ccgggcgcga catcccgatc
     4441 gtggccacac tcgaggaagc gctcgccacg gagccgcggc cggacacgct cctgatcggg
     4501 atcgcgccga cgggcggcaa gctgcccgac agctggcgga cgacgatcct ggcggcgatc
     4561 cgggcgggcc tcgacgttca ctcgggcctc cacacattcc tcgccgacga cccggagttc
     4621 gaggcggcag cgcatctggc cgggaccgag atcctggact atcggaggcc gccaggtcgc
     4681 atggagacgt cggtcgggcg gcgccatgga ccgggcaagc gggtgatcct gaccgtcgga
     4741 acggactgcg ccatcggcaa gatgtcggtc gcgctcgagc ttcgggcagc cgcccgcgcg
     4801 gccggccgct ccacctcgtt cgtcccgacc gggcagaccg ggatgatgat cgaaggctgg
     4861 ggcgcggcgg tcgaccggct gatcagcgac ttcgcccagg ggacggtcga gtggctggtc
     4921 gaggagggcg agcgccgggg cgattgggtg atcgtcgagg gccagggttc gctcgatcac
     4981 ccggcgtact cgtcggtcac gctggccctg atccacgggg ccacgccgca cgcgatgatc
     5041 ctcgtccaca aggccggcca gaccgagcac gacttcgacc acctgcccga tgcgtcattc
     5101 ccgatcgccg acctgggctc gttcatcgac ctccacgagc gcgtggcggg gctcgtcgcg
     5161 ccgtcgaagg tcgtggccat cgccctgaac acctcggcga tcgcatcggc cgacgacgcc
     5221 cgcgtcgaga tcgccagggt cgccgcggac accggactgc cggtcgacga tccggtgcgg
     5281 ttcggtgccg ggccgctgtg gacccggatc gagcaggcgg tcgacgatct cccgtgggtc
     5341 gagggtcgct gacgtggcgc tccacctgag ccaggagatc ctccgtctcg gcctgcgcga
     5401 tccgttccgg atcgcccggt ccgaccatga cgccggctcg gccgtgacga cggtcgtcat
     5461 cgagctccgc gacgaccggt tcacggggca cgtcgggatc ggcgagggct atccggatcg
     5521 attctatggc gacacggtcg agacgatggc ggcggtcttc ccgctcctcc tcgatgccgt
     5581 cggcgagctc gagccgactc gcgacggtct cgtcgctgcc ggggcgcgga tggctgccgc
     5641 gatcgcccac cacggcggcg cgaagtgcgc gctcgacatc gcgctccacg atctcgtcgg
     5701 caaggtcaag ggcatgtcca tcgccgacct gctcgacctc cacgatccga tcccgccgac
     5761 cgacttcacg ctcggcctcg atgagccggc ggtcgtcgca gagcgagccc gccgagcggc
     5821 ccattttccg gcgctgaaga tcaagtgcgg cggacctgcg gatctcgcga cgctcgaagc
     5881 cgtccgggcg gtctacgacg gcccactgcg cgtcgatgcc aacaccggct ggcagccgga
     5941 cgacgccctt gccctcctgc cgcagctcga gcggctcggg gtcgagctca tcgagcagcc
     6001 gtttccggct cgccggctcg accagctccg ctggcttcag gatcgttcgc cgctgccgat
     6061 cgttgccgac gagagcgcgg tcacggtcga ggacctcgaa ggcctggtcg gggtcgtggc
     6121 gggggtgaac gtcaagctcg ccaagtgcgg cggcgtcggc ccggcgcggg cgatgctggc
     6181 gcgcgcccgc gagctcggtt tccggacgtt cctcggctgc atggaggaga cgtcggtggc
     6241 gatcgcggcg tcggcggccg tggcatcgct cgcggactgg gtcgacctcg acggcaacct
     6301 cctcctcgcc gccgacccgt tcacgggcct cgagctcgat gacgactgtc ggtggcgact
     6361 ggcgggcgcg cccggcctcg ggatccggcg acagcctggg tgagggccgg agcgacagcg
     6421 gcgcccggtc gtcggccggc cggacccagg atcggacagg cgttcaccga acgtccgttt
     6481 catgtggaca agttcgtgga cgagttcgtg gacaaacccc cttcgcgggg cgggccgggc
     6541 agcctacgat aggcgtccat cgcgacccga ggccctccgt gagggacggg gcctcgatcg
     6601 atgggtcccc acgggacgtc cgggccgact gcatccgtcg gcagccggac gctggccgcg
     6661 gacagtcacg cgctgaagca agaatggagg gtcggccatc gatgaaccga acgaacgctg
     6721 gcgaacgtgc gtttgccgcc gcgcctgccg tggaaaccga agccattgac ttcgtccggt
     6781 tctgccatcg ccggcgtcgc gtcggctggc cggagctgta tgacgagatg tgcgccgtcg
     6841 cgggtcgagg cctgtaccgg ggctatggag ccgaggagct ctcgtcgatc gggatcgggc
     6901 tcgggctctt ccagatgccg gccctggccg ggctcgtcgc ccaggtcgtc gccgaggacg
     6961 tcgagcgcag gcgtcggagc gccgaggcgg tgcgagcgag cctcgccgcg caggcggccg
     7021 acctggtgac cgtggccgaa ggtggcgttg actgcgccga gagtggcgtg tcgcccacgc
     7081 ggaggaccga tccggagccg gccgacgccg gaccgccgga gcatggagcg atcaggttcg
     7141 cggtggctgc cggagcctga ccgtcgcaga gcgattgtcg cgtcgctatc gacgcttgga
     7201 gggccctcac cggtcagggt gagggccctt ccatttgggc tgccgcgcct tcgcggccga
     7261 tcgcggcgtt gacccctgcg gtcctcgcaa gctcctatgt cgtgtcaagc cgcgagggcg
     7321 gcccagcgag cttgaccacc ttggaggagg cggaacatcg gacgagcgat ccggtgcttg
     7381 agacagcgga gcgcttcttt ccagctcatg ccctcggagc gcttccgcgc gatgtacgcc
     7441 cgagcgggtg ggtgatgcca ggcctgaacc agagcgatcg tgtacagcgc ccggttcagg
     7501 cg
//
LOCUS       DATAIW010000035         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_209094, whole genome shotgun sequence.
ACCESSION   DATAIW010000035 DATAIW010000000
VERSION     DATAIW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209094"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHM48_01275"
     CDS             complement(<1..884)
                     /locus_tag="VHM48_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2754059.1"
                     /translation="MTTTRDAANEGTGNGAGHGATAQHVAVTGRVQPTSPLRRRVAVL
                     RRALRYESHRLHDRRRILAMTLLIITFGCLAAALVARGEPAGADARAYWAAVRIWLGG
                     GDPYRPTGPFLPYVYAPWMLPLFAPWALMPWDVAWFVWRGGTVLVLLWTIHWAYSRRP
                     LQTAVLVALLGFPIGANLDTGNINLPLTLLLWGAQFSGPRVAGVLWAVASWMKWVPVV
                     FLAVLRPRARLWGLLFLAVSVLLSLATLPGTIAQLEALVSFGRRPIRLDYIVFLWALV
                     PPLWRRPDPFGWLRRDWW"
     gene            1025..1867
                     /locus_tag="VHM48_01280"
     CDS             1025..1867
                     /locus_tag="VHM48_01280"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX2754060.1"
                     /translation="MSDGTVIIIGGAEDKIRDRVILNRFVALAGGRDATIVVISTASS
                     LGTEAGERYRQIFADLGVQTVRPLHATTRPQANDETVALALRDATGVFLTGGNQLKLA
                     STIGGTRLADAMLDRFRHGAVMAGTSAGASAMSSHMIAFGASGATPKHRMAQIAAGLG
                     MLPGVIVDQHFQQRNRLGRLLSLIAQNPSLLGLGVDEDTAGVVGPDHVMEVIGRGSIT
                     VVDGAASETDAWEIRGHKPLMISGVVLHSLPSGYRFDLRRRHRLATPTLHTIQGGGEE
                     AASS"
     gene            2194..>4570
                     /gene="cphA"
                     /locus_tag="VHM48_01285"
     CDS             2194..>4570
                     /gene="cphA"
                     /locus_tag="VHM48_01285"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2754061.1"
                     /translation="MAQSAPKGRSAKQSATGVTDAPASGPAGAGVPAAPDAVATKPRR
                     RSIDGPPTGSRPEPTLRILETRILRGPNYWAREPVVRMVVDLGVLEDFPSNKIPGFTD
                     ALVNLLPSLEDHACSLGRRGGFITRLREGTWAGHIAEHIALELQNLSGTDVRHGKTRS
                     TGEHGRYNVIFEFREESVGTEAGKLAVALVNHLVAPDDEAVNLDFMAELERLIRLAET
                     SAFGPSTQAILDEAASRDIPYIRLDRHSLVQLGQGVHQQRIRATMTSMTSAIGVDIAS
                     DKSLTNRLLDSAGLPVPKSAVVNTEEATVEAARRIGFPCVVKPLDGNHGRGVHLDLRS
                     DEAVRAAFHGARAQSRSGDLVVESYVAGNDYRCLVIGGKVAAIAQRVPASVIGDGERS
                     IRQLVDLANADPRRGIGHEKVLTRIKVDEPAEALVREQGLELDGVPAEGTLVKLALTG
                     NMSTGGTSIDRTIEAHPDNVEIAETAAQIVGLDIAGIDFICPDIATPVRETGGAIVEV
                     NAAPGFRMHTHPTEGEPQYVARPVIDGLFPPGSPSRIPIVAVTGTNGKTTTVRMIAHI
                     LKLKGKRVGMTSTDGIVIDGRMIKKGDMSGPKSAQMVLQNPTVDTAVFEVARGGILRE
                     GLGYDRNDVAVVTNVTGDHLGIGGIDSIGQLANVKAVVVEAVPRSGTAVLNADDGLVY
                     RMGRHCAGKVVLFSMATQKGEDGYDRIDGHTARGNAAFILQPGPEGELIVLRHGPRTM
                     PVLYTHLIPATFGGRARMNVANALAAAAAAWAAGAHLHDIRQGLRTFSTSFF"
BASE COUNT          743 a         1667 c         1571 g          589 t
ORIGIN      
        1 ctccaccagt cgcgccggag ccagccgaat gggtccggcc ggcgccagag cggcggcacc
       61 agcgcccaca ggaacacgat gtagtcgagg cggatcgggc gcctgccgaa gctgaccagg
      121 gcttcgagct gggcgatcgt gccgggcagc gtcgcgaggc tcaggaggac cgagacggca
      181 aggaacaaga ggccccacag gcgcgcccgc ggtcggagca cggcaaggaa gacgaccggg
      241 acccacttca tccagctcgc gactgcccag aggaccccgg cgacgcgggg accgctgaac
      301 tgggctcccc acagcagcag cgtcaacggc aggttgatgt tgccggtgtc gaggttcgcc
      361 ccgatcggga acccgaggag ggcgacgagg acggccgtct ggagggggcg ccggctgtac
      421 gcccagtgga tcgtccagag gaggacaagg accgttccgc cgcgccacac gaaccaggcg
      481 acgtcccacg gcatcaacgc ccagggcgcg aacagcggca gcatccaggg tgcgtacacg
      541 tacggcagga acggcccggt cggccgatac ggatcgccgc cgccgagcca gatccgaacg
      601 gcggcccagt acgcccgcgc gtcggcgcca gccggttcac cccgggcgac cagagctgcg
      661 gcgaggcacc cgaaggtgat gatcagcagc gtcatcgcga ggatccgccg ccggtcgtgg
      721 agccggtggg actcgtagcg gagcgcgcgc cgaaggacgg cgacgcggcg ccgcagcggg
      781 ctcgtcggtt ggacgcgccc cgtgacggcc acgtgctggg cggtggcgcc atgacccgcg
      841 ccgttgcccg tgccttcgtt tgccgcgtcc ctggtcgtgg tcatttcacg gcattctggc
      901 agacgtccac gccgcgcccg tcatcgcgac ctgaaccgca acatccgcca ggcgccgctg
      961 tcggatcgcc gaacggtccc ccgcgctatc cttccaacga tccgagacga gggagcgcgc
     1021 gtccgtgagc gacgggaccg tcatcatcat cggcggggcg gaggacaaga tccgcgaccg
     1081 ggtcatcctc aaccgattcg tcgcgctcgc cggcggccgc gatgcgacga tcgtcgtgat
     1141 ctcgaccgcg tcgagcctcg gcaccgaggc cggcgaacgg tatcgccaga tcttcgccga
     1201 cctcggcgtc cagacggtcc gaccgctcca cgcgacgacg aggccccagg ccaacgacga
     1261 gacggtcgcc ctcgcccttc gcgacgccac gggcgtcttc ctgacgggcg gcaaccagct
     1321 caagctcgct tcgacgatcg gcgggacacg cctggccgac gcgatgctcg atcggttccg
     1381 ccacggggcg gtcatggccg ggacgtcggc cggagcatcg gcgatgagca gccacatgat
     1441 cgcgttcgga gcgtccggtg cgaccccgaa gcatcggatg gcccagatcg ccgccgggct
     1501 ggggatgctc ccgggggtca tcgtcgacca gcacttccag caacggaacc ggctcgggcg
     1561 attgttgtcg ctgatcgccc agaacccgtc actgctcggg ctcggcgtcg acgaggacac
     1621 ggccggcgtc gttggtcccg accatgtgat ggaggtcatc ggccggggca gcatcacggt
     1681 cgtcgatggg gcggcatccg agaccgacgc ctgggagatc cgcggccaca agccgttgat
     1741 gatcagcggc gtcgtgctcc attcgttgcc gtccggctac cgcttcgatc ttcgccgccg
     1801 ccaccgcctg gcgacgccga cgctccacac gatccagggt ggcggcgagg aagccgcctc
     1861 gagctaggcg acgggtgccg ccgggggccc tcgccgccag cggcatggac gcggttggtc
     1921 cggagggcgg accaacggac ggccgacggc cggaccgatg tcccgatcga ccggttcagc
     1981 cgtccagtgg tccggctcgc ggaccatcgg gcgggcggaa tgcccattgc accccgagga
     2041 tcgacatcca gacgtcggtt ggtccgcccg gcggaccacc gccccatgcc cggcgctcgt
     2101 tcacagagcg ggaggacccg tggcggccac ccggacccgc cgggtacact cgggacccgg
     2161 ctgacgggag cgagagggcg gcaggagggc tccgtggcgc aatcggcacc gaagggtcgt
     2221 tcggcgaagc agtcggccac gggcgtgacg gacgccccgg cgtccggtcc cgccggcgcc
     2281 ggcgtcccgg ccgcgcccga cgccgtggcg acgaagccgc gccgtcgctc gatcgacggt
     2341 ccgccgaccg gctcccgacc ggagcccacc ctccggatcc ttgagacccg gatcctgcgc
     2401 ggcccgaact actgggcccg cgagccggtc gtccggatgg tggtcgacct cggcgtcctc
     2461 gaggacttcc cctcgaacaa gattccgggt ttcaccgacg ccctcgtcaa cctgctgccg
     2521 agcctcgagg accacgcctg cagcctcggc cggcgcggcg gcttcatcac ccgtcttcgc
     2581 gagggtacgt gggccggcca catcgccgag cacatcgccc tcgagctgca gaacctctcg
     2641 gggaccgacg tccgccacgg caagacccgc agcaccggcg agcacggccg ctacaacgtg
     2701 atcttcgagt ttcgcgagga gagcgtcgga acggaggccg gcaagctcgc cgtcgccctc
     2761 gtcaaccacc tggtggcgcc agacgacgag gcggtcaacc tcgacttcat ggccgagctc
     2821 gagcggctga tccggctcgc cgagacatcg gccttcgggc cgtcgaccca ggcgatcctc
     2881 gacgaggcag cgagccgcga catcccgtac atccggctcg atcgccactc cctcgtccag
     2941 ctcggccagg gcgtccacca gcagcggatc cgggcgacga tgacgtcgat gacgtcggcg
     3001 atcggcgtcg acatcgcgag cgacaagagc ctcacgaatc ggctcctcga ttcggccggt
     3061 ctgccggtcc cgaaaagcgc cgtcgtcaac acggaggagg cgaccgtcga ggccgcccgg
     3121 cggatcggct tcccgtgcgt ggtcaagccg ctcgacggca accatggtcg gggcgtccac
     3181 ctcgacctcc gcagcgacga ggcggtccgg gcggccttcc acggcgcgcg agcccagagc
     3241 cggagcggcg atctcgtcgt cgagagctac gtcgcgggca acgactatcg ctgcctggtg
     3301 atcggcggca aggtcgccgc gatcgcccag cgcgtcccgg ccagtgtcat cggcgacggc
     3361 gagcggagca tccgccagct cgtcgacctg gccaacgccg acccgcgccg cggcatcggc
     3421 cacgagaagg tcctcaccag gatcaaggtc gacgagccgg ccgaggcgct cgtccgcgag
     3481 cagggcctcg agctcgacgg cgtcccggcc gaaggcacgc tcgtgaagct cgccttgacg
     3541 ggcaacatgt cgaccggcgg cacgtcgatc gaccggacga tcgaggccca ccccgacaac
     3601 gtcgagatcg ccgaaacggc cgcccagatc gtcggcctcg acatcgccgg catcgacttc
     3661 atctgcccgg acatcgccac gcccgtccgc gagacgggtg gcgcgatcgt cgaggtcaac
     3721 gccgcacccg gcttccggat gcacacccac ccgaccgaag gcgagccgca atacgtcgcc
     3781 cgccccgtca tcgacgggct cttcccgccg ggctcgccgt cacggatccc gatcgtggcc
     3841 gtgaccggca cgaacggcaa gacgacgacc gtccggatga tcgcccacat cctcaagctc
     3901 aagggcaagc gggtcgggat gacctcgacc gatgggatcg tgatcgacgg ccggatgatc
     3961 aagaagggcg acatgtccgg cccgaaatcg gcccagatgg tcctccagaa cccgaccgtc
     4021 gacaccgcgg tgttcgaggt cgcccgtggc ggcatcctgc gcgagggcct cggctacgac
     4081 cgcaacgatg tcgcggtcgt cacgaacgtc accggcgacc atctcgggat cggcgggatc
     4141 gattcgatcg gccagctcgc gaacgtcaag gcggtcgtcg tcgaggccgt gccgcggtcg
     4201 ggaacggcgg tcctcaacgc ggacgacggc cttgtctacc ggatgggccg ccactgcgcc
     4261 ggcaaggtcg tcctgttcag catggcgacc cagaagggcg aggacggcta cgaccgcatc
     4321 gacggtcaca cggcccgcgg caacgccgcg ttcatcctcc agccaggtcc ggagggcgag
     4381 ctgatcgtcc tccgccacgg gccgcggacg atgccggtcc tctacaccca cctgatcccc
     4441 gctaccttcg gagggcgggc ccggatgaac gtcgccaacg ctctcgctgc ggccgcggcc
     4501 gcgtgggccg cgggcgccca cctccatgac attcgccagg gcctccggac gttctcgacc
     4561 tcgttcttcc
//
LOCUS       DATAIW010000036         8682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1235, whole genome shotgun sequence.
ACCESSION   DATAIW010000036 DATAIW010000000
VERSION     DATAIW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1544
                     /locus_tag="VHM48_01290"
     CDS             207..1544
                     /locus_tag="VHM48_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2754062.1"
                     /translation="MIDILILVLLTFLEGFFVAAEIALVSIRRSRVEQLVDEGRSGAR
                     RVRRLLEEPGRFLAVSQLGLTVIGFFASAYAAVSLVQKFSDILVGFGIKKDGGADTIA
                     LVVVTVILALFTIIFAELVPKTLALANTERFAITLSLPIEFLARALGPLIRVLTGVTA
                     WITKLFGANVSNEAQITAEELRLIVERGGEQGVLEAEEEQMINSIIELGSRRLHEVMI
                     PRIAIASLSATATLEEAIDKVVEEGHSRIPVYEESIDEVVGILYAKDLLPILKAGSGP
                     RPELRSLLRAPVFVPESMTIDDLLHEFQRRKVHIAIVLDEYGGTAGLLTIEDLLEEIV
                     GEIQDEYDTEEPMVVRLSDDEFRVDGRADVDDLAELFDTELRLEDEDEYDTVGGLIYH
                     RIGGVPSPGDVVEVDGLELTVETIDGRRVGKVLVVRKRRDEDGEPYPDEDGDR"
     gene            1648..2040
                     /locus_tag="VHM48_01295"
     CDS             1648..2040
                     /locus_tag="VHM48_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754063.1"
                     /translation="MTTIDDMVVDVGDQTSAAEREAWPDRARTRAVFDAGAFEPEVAA
                     AIARYHTLRAISDAYPGWRGVLRVRGLSITWPEWRQLPAADRKFMIERLAAWSPAVVI
                     NGSTVAQPRIQLPVLELVFGSRIRTDAG"
     gene            complement(2281..3306)
                     /locus_tag="VHM48_01300"
     CDS             complement(2281..3306)
                     /locus_tag="VHM48_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2754064.1"
                     /translation="MTTAQPVLDAAALIADTEAEILRIVRDRDPSTVGVYEMVRYHLG
                     LDGSGASAGKRMRPLLGLLAYASIAGDYRPALPGAAAVELGHNFSLVHDDIEDGDRER
                     RHRPTVWALHGVAQAINTGDMLFSLSRMALHRLTDLGFSDAKVLRLMRLYDQTCVALC
                     EGQYIDIATSERDDMMSVELYFDMIGRKTAALIAASIEAGALLASDDDEVIARYRGFG
                     WALGLAFQLNDDLLGIWGAEQATGKKPSDIARRKKTLPVIYAFEHAGPEDRERLAALY
                     GNGGPTGDDVAEIKAILERSGARDFTRTEARRHRDEALAELDAAGVVDPEARARLEEI
                     IVTVIAA"
     gene            3514..4416
                     /gene="recO"
                     /locus_tag="VHM48_01305"
     CDS             3514..4416
                     /gene="recO"
                     /locus_tag="VHM48_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2754065.1"
                     /translation="MPIPRRYSTDAIVLSRFDYGEADRILTLITPTGGKLKAIAKGIR
                     RQKSRIGGSLEPFAELHVVLARGRTFEVVTQVSVNHAWLNMRDNLESAATAWYLAELA
                     DRSLEEHRGAEPVYAILRRAYELLDAGMAPGRVARWYEMHLADELGVRPEVDRCVECD
                     RMLEADGRFRWVPPLGGVLCDRCPGPPHDRTGLTLDGLKLLKAYQRQDVEALSGLRLA
                     EATEREVENAMRDFIRNVLEREARSLAFLDEIRTPEATERHERHDRAVAERDAADREA
                     AASEAVPAEFAIEPVAVETAGAPG"
     gene            4416..4925
                     /locus_tag="VHM48_01310"
     CDS             4416..4925
                     /locus_tag="VHM48_01310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754066.1"
                     /translation="MPTVELRDIVTEDDVEAVMGLRRGPGQERHLGSMISHFEDAIAD
                     ARACPRMWAVHAGDTGELVGFVMISDNVPAETLAADDDIVGPYFLWRLLIDHRQQGKG
                     YGGATIDAVVAYVRTRPDADVLLTSCKAGEGSPQPFYLRYGFEKTGEVKWGEDLLGLD
                     LRAPIGGLA"
     gene            4868..7051
                     /locus_tag="VHM48_01315"
     CDS             4868..7051
                     /locus_tag="VHM48_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754067.1"
                     /translation="MGRRPPRTRSPGADRGPGLSYHPPIIPRGSRLRRTQRPGIEGRE
                     SGLSGNPDIPDPPPEGPTDLRGRLIRARDAVRALMGTLPRVLGLVWSASKVLTIGLAT
                     ATVLAGIVPAITAYIAKLLIDAVVRAIAVNANPSLPHTITVGPLTLDAVGSVVLLAAA
                     QFTIYAVSSFLSTLRNVCQQLLQERVTNTIQLEIMDHAAKLDLAFFEDSTSYDLLRRA
                     QQGASSRPLFMVSGVFGLIQTAIAFISMIALLIALSPILALVALVAPIPAFIADTRYG
                     WRGYSFARWASPLRRRMDYLTTLVTTDTYAKEVKLFGLGPYFIDRFHQLSTVYQDRQR
                     GLVVTRYFAGFIWSTITTLAGSLTYLYVALQAIAGKLTLGDLTLFTQAASSVQGSVQG
                     LLGGAGSMYENNLYLNDLFELLATPVGIEGAAGDGSIATERRTTPTGAPALDLAATRG
                     ELTFDHVTFRYPGREEDAVADVSFTIAPGETVAVVGRNGAGKSTLIKLVTRLYDPTGG
                     RILLDGVDLRELEPAAVRQLIGGMFQDYVTYQASAAENIGLGELGRLEDREAVEEAAR
                     KGGADSLLDRLPDGWDTPLGKWFDGGVELSGGEWQKIALGRAFMRDARILLLDEPTSA
                     LDAQAEFELFARLRSLARGRTAIYISHRFSTVRQADRILFLEHGRLVEQGTHQELMAL
                     GGRYAQLFTLQAAAYTGEPIDDLLAADEAEARTSDQAEPFAERSA"
     gene            7051..8247
                     /locus_tag="VHM48_01320"
     CDS             7051..8247
                     /locus_tag="VHM48_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2754068.1"
                     /translation="MAPSRRSVVAAGLTAAALTVGGPILRRTIGERLSARTERRLNGV
                     ADPGPYLVPPAATDLHERLTIVDLHADSLLWGRDLLARASRGHVDVPRMIEGNAALQV
                     FAVTTKVPRHMSYERNDDRSDDISLVALVQGWPRATWRSLLARAEHQAGRLQRFAAAS
                     DGRLTLVRSATDLDGFLARRAAEPSLVAGLLAIEGAHALDDDPANVERLAAAGYRMVG
                     LAHFFDNAFAGSAHGVAKGGLTPAGRELVAELERRRMIVDLAHASAATIDDVLAIVTR
                     PVVASHTGVRGVADHTRNLSDEQLRGIAATGGMVGIGFWPTACGGDDAASIARSIAYA
                     VTVVGADHVGLGSDFDGAVGTPFDVSGMAVLTAALLAQGMDEAAIAAVMGGTAVRLLR
                     AALPPA"
     gene            complement(8287..>8682)
                     /locus_tag="VHM48_01325"
     CDS             complement(8287..>8682)
                     /locus_tag="VHM48_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754069.1"
                     /translation="VPVAWAVLGPVGAAVSSLVAGFVAGVIAGTGRRAIVAVAFGALP
                     AYWIDLVMMPPSTQEFGGFVQMAAGLAIALLAGLALLGGALGVAGARAEHLDPFRSAH
                     RRMALGVAALATVAGWLWWGVIVAATAPA"
BASE COUNT         1281 a         3093 c         3045 g         1263 t
ORIGIN      
        1 cgtcgggctg acggtcgagg atgacggacg cctcctcggg atcaacgacc gggccgagct
       61 cgcccaggcc gagtgggacc tccggacgaa gatcaacagc gcccacatgc gagccggagt
      121 gacgatccgc gacccctcga accagaaccc gagccggagc ccgagccgaa gcctgacacc
      181 atgcccggct ccgacggagc cgccggatga tcgacatcct gatcctggtc ctgctgacgt
      241 tcctcgaggg cttcttcgtc gccgccgaga tcgctctcgt ttcgatccgc cggagccgcg
      301 tcgaacagct cgtcgacgag ggccgatcgg gcgcacggcg cgtccgccgg ctgctcgaag
      361 agccgggccg cttcctcgcc gtgtcgcagc tcgggctgac cgtcatcggc ttcttcgcgt
      421 cggcatacgc cgcggtcagc ctggtccaga aattcagcga catcctggtc ggattcggga
      481 tcaagaagga cggcggggcc gacacgatcg cgctggtcgt cgtcaccgtc atcctcgccc
      541 tgttcacgat catctttgcc gagctcgtcc cgaagaccct cgctctcgcc aacaccgagc
      601 ggttcgcgat cacactgtcg ctgccgatcg agttcctcgc ccgcgccctc ggcccgctga
      661 tccgggtcct gaccggcgtc accgcctgga tcacgaagct cttcggggcg aacgtctcca
      721 acgaggccca gatcaccgcc gaggagctcc gcctgatcgt cgagcgcggc ggcgagcagg
      781 gcgtcctcga ggccgaggag gagcagatga tcaactcgat catcgagctc ggctcgcgcc
      841 gcctccacga agtgatgatc ccgcggatcg cgatcgcctc gctgtccgcc acggcgaccc
      901 tcgaggaggc gatcgacaag gtcgtcgagg agggccacag ccggatcccg gtctacgagg
      961 aatcgatcga cgaggtcgtc ggcatcctct acgccaagga cctccttccg atcctcaagg
     1021 cggggtccgg tccgcgcccg gagctccgct cactgcttcg cgcgccggtc ttcgtgcccg
     1081 agtcgatgac gatcgacgac ctgctccacg agttccagcg gcggaaggtc cacatcgcga
     1141 tcgtcctcga cgagtacggc gggaccgccg gcctgctcac gatcgaggac ctcctcgagg
     1201 agatcgtcgg cgagatccag gacgagtacg acacggagga gccgatggtc gtccgcctgt
     1261 ccgacgatga gttccgggtc gacgggcggg cggatgtcga cgacctcgcc gagctgttcg
     1321 acaccgagct ccggctcgag gacgaggacg aatacgacac ggtcgggggg ctgatctatc
     1381 accggatcgg cggcgtgccg tcacccggcg acgtggtcga ggtcgacggg ctggagctga
     1441 cggtcgagac gatcgacggc cgccgcgtcg gcaaggtcct cgtcgtccgc aagcgccgcg
     1501 acgaggacgg cgagccgtat cccgacgagg acggcgaccg ctaaggtcgc ctcagacgcg
     1561 atgccgatcg tcgatctcgc tatcgggcct gggtcgatca cgacgcgcat tgccgctcgt
     1621 ggaacatctc ggcgctggct ggaccaggtg acgacgatcg acgacatggt cgtcgacgtc
     1681 ggcgaccaga ccagcgcggc agagcgcgaa gcatggccgg atcgagcaag aacgcgcgcc
     1741 gtgttcgatg ccggagcatt cgagcccgag gtggcagccg ccatcgcccg ataccacacg
     1801 ctgcgcgcga tcagcgacgc gtacccgggg tggcgcggcg tactccgggt ccggggtctg
     1861 tcgatcacct ggcctgagtg gcggcagctc cccgccgctg accggaagtt catgatcgag
     1921 cgcctcgcgg catggtcgcc ggctgtcgtg atcaacggct cgacggtggc tcaaccacgg
     1981 atccagcttc cggtgctcga gctcgtgttc ggcagccgga ttcgaacgga tgccggatag
     2041 gcccttgcgc ccaggcgaca gccgcctacg gccgtacgcc tcttgcgctg atccggctcc
     2101 gcgcacgctc gggtgatcga cgcagtctcg tcccccaggc gagtctcgcc tggacgctgg
     2161 agattccgcg ttcgacggcc gataccaggt gagcgtcgcc tggacgcaag agattccgag
     2221 cttggctgcc gataccaggt gagcgtcgcc tgggcgcaag aaatgtcgtc tcggcgaccg
     2281 ctacgcggcg atcaccgtga cgatgatctc ctcgaggcgg gcgcgggcct cgggatcgac
     2341 gacgccggct gcgtcgagct cggccagggc ttcgtcgcga tggcggcggg cctcggtccg
     2401 ggtgaagtcg cgggctccgc tgcgctcgag gatcgccttg atctcggcga cgtcatcgcc
     2461 ggtcgggccg ccgttgccgt agagcgcggc gagccgctcg cggtcctctg gcccggcgtg
     2521 ctcgaacgcg tagatcaccg gcagcgtctt cttgcgccgg gcgatgtcgg acggcttctt
     2581 gccggtcgcc tgctcggcgc cccagatccc gaggaggtcg tcgttgagct ggaacgcgag
     2641 cccgagcgcc cagccgaagc cacgatagcg ggcgatcacc tcgtcgtcat cggaggcgag
     2701 cagggcgccg gcctcgatcg aggcggcgat cagggccgcc gtcttgcgcc cgatcatgtc
     2761 gaagtagagt tcgaccgaca tcatgtcgtc gcgctcgctc gtcgcgatgt cgatgtactg
     2821 gccctcgcac agcgcgacgc aggtctggtc atagaggcgc atcaggcgga ggaccttggc
     2881 gtcgctgaag ccgaggtcgg tcagccgatg aagcgccatc cggctgaggc tgaagagcat
     2941 gtcgccggtg ttgatcgcct gggcgacgcc gtggagggcc cagacggtgg gccggtgccg
     3001 cctctcccga tcgccgtcct cgatgtcgtc gtggacgagg ctgaagttgt ggccgagctc
     3061 gaccgcggcg gcgcccggca gggccggccg gtaatcgccc gcgatcgacg cataggcgag
     3121 cagaccgagg aggggacgca tccgcttgcc ggccgacgcg ccggaaccgt cgaggccgag
     3181 gtggtagcgg accatctcgt agacgccgac ggtcgaggga tcacggtcgc ggacgatccg
     3241 gaggatctcg gcctcggtgt cggcgatcag ggcggcggca tcgaggaccg gctgggcggt
     3301 ggtcatcgct ggcatggtag cgggattttc gcaaacatgc gagccgcaag tagcctgttg
     3361 ccggggtgca gcggcaaaga aacggcgccg gtccggggga cgcaaccgca ccggcgcctg
     3421 acggaggtac gccgcgcgcc gcgccctgga tgaatcgatc caccgaccac gttcacgacg
     3481 gcccctgacc agcggaggtg ctaccgtccg aatgtgccga tcccgcgccg atattcgacc
     3541 gatgcgatcg tgctctcgcg gttcgattac ggcgaggcgg accgcatcct gacgctcatc
     3601 acgccgaccg gcggcaagct caaggcgatc gccaagggca tccgccgcca gaagtcgcgg
     3661 atcggcggtt cgctggagcc gttcgccgag cttcacgtgg tgctcgcccg cggtcggacg
     3721 ttcgaggtcg tcacccaggt cagcgtcaac cacgcgtggc tgaacatgcg cgacaacctc
     3781 gagagcgcgg cgaccgcgtg gtacctcgcg gagctcgccg accgctcgct cgaggagcac
     3841 cgcggcgcgg agcccgtcta cgcgatcctc cgccgggcct acgagctcct cgacgccggc
     3901 atggcgccgg gtcgggtcgc ccgctggtac gagatgcacc tcgccgacga gctcggcgtc
     3961 cggcccgagg tcgaccgctg cgtcgagtgc gaccgcatgc tcgaggccga cgggcgcttc
     4021 cgctgggtcc cgccgctcgg cggcgtcctg tgcgaccgct gtcccgggcc gccccacgac
     4081 cggaccggac tgaccctcga cgggctcaag ctgctcaagg cgtaccagcg ccaggatgtc
     4141 gaggcgctgt ccgggctgcg cctcgccgag gcgaccgaac gcgaggtcga gaacgcgatg
     4201 cgcgacttca tccgcaatgt cctcgagcgc gaggccagat cgctcgcgtt cctcgacgag
     4261 atccggacgc cggaggccac ggaacggcat gagcgccacg accgggcggt cgcggaacgc
     4321 gatgccgccg accgcgaggc tgccgcgagc gaggccgtgc cggctgaatt cgcgatcgag
     4381 ccggtcgcgg tcgagaccgc gggggcgccc ggctgatgcc gaccgtcgag ctccgcgaca
     4441 tcgtcaccga ggacgacgtc gaggccgtca tggggctgcg ccgaggcccg ggccaggagc
     4501 gccacctcgg gtcgatgatc agccacttcg aggacgcgat cgccgacgcg cgggcctgcc
     4561 cccggatgtg ggcggtccac gccggcgaca cgggtgagct cgtcggcttc gtgatgatca
     4621 gcgacaacgt ccccgccgag accctggccg ccgacgacga catcgtcggg ccgtacttcc
     4681 tgtggcggct cctcatcgac caccgccagc aggggaaggg ctacggcggg gccacgatcg
     4741 acgcggtcgt ggcgtacgtc cggacccgcc ctgacgccga cgtcctcctg acgagctgca
     4801 aggccggcga gggctcgccg cagccgttct acctccgcta cgggttcgag aagaccggtg
     4861 aggtcaagtg gggcgaagac ctcctcggac tcgatctccg ggcgccgatc gggggcctgg
     4921 cctgagctac cacccgccga tcatcccccg cggcagccgg ctccggcgaa ctcagcggcc
     4981 cgggatcgag ggtcgcgaga gcggtctctc gggcaacccc gacatccccg atccaccgcc
     5041 cgagggcccg accgacctgc gcggccgcct gatccgggcc cgcgacgcgg tccgggcgct
     5101 gatggggacg ctgccgcggg tcctcggcct ggtctggtcg gcgagcaagg tcctgacgat
     5161 cggcctcgcg accgcgacgg tcctggccgg catcgtgccc gcgatcacgg cgtacatcgc
     5221 caagctcctg atcgacgcgg tcgtccgggc catcgcggtc aacgccaacc cgagcctgcc
     5281 ccacacgatc acggtcggcc cgctgacgct cgacgccgtc ggcagcgtcg tcctgctcgc
     5341 ggcggcccag ttcacgatct acgctgtctc gtcgttcctg tcgaccctcc ggaacgtctg
     5401 ccagcagctc ctccaggagc gggtcacgaa cacgatccag ctcgagatca tggatcacgc
     5461 cgcaaagctc gacctcgcct tcttcgagga ctcgacctcc tacgacctcc tccgacgggc
     5521 ccagcagggg gcgtcgagcc ggccgttgtt catggtcagc ggcgtattcg ggctgatcca
     5581 gacggcgatc gcgttcatct cgatgatcgc gctgctgatt gccctcagcc cgatcctcgc
     5641 gttggttgcc ctcgtcgccc cgatcccggc gttcatcgcc gacacgcgct acggctggcg
     5701 cggctacagc ttcgcccgct gggcctcgcc gctccgccgc cggatggact acctgacgac
     5761 cctcgtcacc accgacacgt acgccaagga agtcaagctc tttggcctcg gcccgtactt
     5821 catcgaccgc ttccaccagc tctcgaccgt gtaccaggat cgccagcgcg gtctcgtggt
     5881 cacgcgctac ttcgcgggtt tcatctggag cacgatcacc acgctcgcgg gctcgctgac
     5941 ctatctctac gtcgcgctcc aggcgatcgc cggcaagctg accctcggcg acctgaccct
     6001 gttcacccag gcggcgtcgt ccgtccaggg ctcggtccag ggcctgctcg gcggggcggg
     6061 ctcgatgtac gagaacaacc tctatctcaa cgacctgttc gagctgctcg cgaccccggt
     6121 cgggatcgag ggcgcggccg gggatgggtc gatcgcgacc gagcgccgga cgacgccgac
     6181 cggcgcgccc gccctcgacc tggccgccac ccgcggggag ctgaccttcg accacgtgac
     6241 gttccgctat ccgggtcgcg aggaggacgc cgtcgccgac gtcagcttca cgatcgcccc
     6301 gggcgagacg gtcgcggtcg tcggccggaa cggggcgggg aagtcgaccc tgatcaagct
     6361 cgtcacccgc ctgtacgacc cgaccggcgg gcggatcctg ctcgacggag tcgacctgcg
     6421 ggagctcgag ccggccgcgg tccgccagct gatcggcggg atgttccagg actacgtcac
     6481 ctaccaggcg agcgcggccg agaacatcgg gctcggcgag ctgggtcggc tggaggatcg
     6541 ggaggccgtg gaggaggcgg cgcggaaggg cggcgccgac agcctcctcg atcgcctgcc
     6601 cgacggctgg gacacgccgc tcgggaagtg gttcgacggc ggcgtcgagc tgtccggcgg
     6661 cgagtggcag aagatcgccc tcgggcgcgc gttcatgcgc gacgcccgga tcctcctcct
     6721 cgacgagccg acgtcggcgc tcgacgccca ggcggagttc gagctgttcg cccggctccg
     6781 ctcgctcgct cgcggccgga ccgcgatcta catctcgcat cggttttcca ctgtccggca
     6841 ggccgaccgg atcctgttcc tcgagcacgg gcggctggtc gagcaaggga cccaccagga
     6901 gctgatggcg cttggcggcc gctacgcgca gctcttcacc ctccaggcgg ctgcctacac
     6961 cggcgagccg atcgacgacc tgctcgccgc cgacgaagcg gaggcacgca cgtcggacca
     7021 ggccgagccg ttcgctgagc gcagcgcctg atggcgccct cccggcggtc ggtggtcgcg
     7081 gccgggctga cggcggccgc gctcacggtc ggcgggccga tcctgcgccg gacgatcggg
     7141 gagcggttgt ccgcccggac ggagcgccgc ctgaacgggg tcgccgatcc gggaccgtat
     7201 ctcgtcccgc ccgccgcgac cgatctccac gagcggctga cgatcgtcga cctccacgcc
     7261 gattcgctcc tgtggggtcg tgacctcctc gctcgggcga gccgcggtca cgtcgacgtc
     7321 ccgcggatga tcgagggcaa cgccgcgctc caggtcttcg cggtcaccac gaaggtgccg
     7381 cgccacatga gctacgagcg gaacgacgac cggagcgacg acatctcgct cgtggcgttg
     7441 gtccagggtt ggccgcgggc gacgtggcgc tcgctcctgg cccgggccga gcaccaggcc
     7501 gggcggctcc agcggttcgc ggcggcctcc gacggccgcc tcaccctcgt ccggtccgcc
     7561 accgatctcg acgggttcct ggcgcgccgc gcggccgagc cgtcgctcgt cgcgggcctg
     7621 ctcgcgatcg aaggcgctca cgccctcgac gacgaccccg cgaacgtcga gcggctggct
     7681 gcggccggct accggatggt cgggctcgcc cacttcttcg acaacgcctt cgccggctcg
     7741 gcccacgggg tggccaaggg cggtctgacg cccgccgggc gggagctcgt cgcggagctg
     7801 gagcggcggc ggatgatcgt ggacctggct catgcctccg ccgcaacgat cgacgacgtc
     7861 ctggcgatcg tgacgcggcc ggtcgtcgca tcgcacaccg gcgtgcgcgg tgtggccgac
     7921 cacacgcgga acctctccga cgagcagctc cggggcatcg ccgcgaccgg cgggatggtc
     7981 gggatcgggt tctggccgac ggcctgcggc ggcgacgacg cagcgtcgat cgcccgctcg
     8041 atcgcgtacg cggtgacggt cgtgggggcg gaccatgtcg gtctgggctc cgatttcgac
     8101 ggcgcggtcg ggacgccctt cgacgtgtcg ggaatggccg tcctgacggc ggcgctcctc
     8161 gctcagggta tggacgaggc cgcgatcgcg gccgtcatgg gcggcacggc ggtccggctc
     8221 ctgcgcgcgg ccctgccgcc ggcctgagcc gccgagagct tcggctggaa ggtcgggccg
     8281 ccgtggtcac gcgggcgcgg tggcggcgac gatcacgccc caccagagcc agccggcgac
     8341 ggttgcgaga gcggcgacgc cgagggccat ccggcgatgc gccgaccgga atgggtcgag
     8401 atgttcggcc cgcgcacccg ccacgcccag cgcgccgccg aggagcgcca acccggccag
     8461 aagggcgatg gcgaggcccg ccgccatctg gacgaaccca ccgaactcct gtgtcgaggg
     8521 cggcatcatg acgagatcga tccagtaggc ggggagggcg ccgaacgcga ccgcgacgat
     8581 ggcacgccgg cccgttccgg cgatcacccc ggcgacgaag ccggcgacga gtgaggagac
     8641 cgccgcgccc acgggcccga ggactgccca cgcgaccggc ac
//
LOCUS       DATAIW010000037         5381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_61010, whole genome shotgun sequence.
ACCESSION   DATAIW010000037 DATAIW010000000
VERSION     DATAIW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61010"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VHM48_01330"
     CDS             <1..643
                     /locus_tag="VHM48_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754070.1"
                     /translation="LAEHDRIGSPLRSELGPGEPPERVEETLRAIGLTAPGELVELFG
                     WRQVRPELLGAPRVAWFWPAQPRHFDEAVDHYRQSIEIGGITPAALEKAIRAGQPPEA
                     TFTGFWRTDWFPWLFGSPEEYAIECRDGASEAPVWRVNWHPDQGFQTKQVGTSVTDFI
                     ERVVELFRAGAYTWDPADHSIVPVDEVFTQRGLDENRPPWDRHYRPASLRSTN"
     gene            complement(610..2031)
                     /locus_tag="VHM48_01335"
     CDS             complement(610..2031)
                     /locus_tag="VHM48_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2754071.1"
                     /translation="MSIEGLGPDLLVLGGTVVTAAGSRRADVAVRTGAIETIETDLSG
                     LAAGSREIVDASGLLILPGAVDVHTHTRVGTDAEPDRFFKDSLAAAFGGTTTFLAFNN
                     PGTGSSAAAHRSILAGIEEFRRVTASDAAVDYALSPAILGGMDDPLTELPAMVDAGVP
                     TAKAFMIFDFRLGDRAIHDAMAILGARGGLLEVHCEDPVLVDAAVAAALQRGDTSPWH
                     HAATRTTETEAVATHRVMAFARATGAPIHVVHLSCAAALTFVAQARADGVRVTAETCP
                     HYLTLTDERYAERDPVECAKSLVTPPLRPAADRDALWAGLAAGDLSMIATDHVPDRVA
                     VEKGEAARGVSFDQLSNGAPGIETLLTLAYGVGVAGGRITLERMVDLVATTPARRFGF
                     ARKGAIEVGRDADLVLFDPAARRTIRAADLHHASDFTPYEGMDVAGAVRSVFVRGRAV
                     IRDGVAAGERGYGQFVERSEAGR"
     gene            complement(2119..3489)
                     /locus_tag="VHM48_01340"
     CDS             complement(2119..3489)
                     /locus_tag="VHM48_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754072.1"
                     /translation="MMLRSGRIVLWALVVALATGCGTSPAVPSASTAPSSSPPSGSIA
                     APPPSATAAPSASDVLDRAAGWRADIDALLDTRDRVHPDGWHGMKRTDRVAAADAVKA
                     RIPRLTDDQTLVELVRLAAMPSWNGRDGHSGIFPFIPGSGTHEYPVRWWRFSDGLVIT
                     AARAPYEDLVGSRVEAVNGRPIADVLALVEPLAPRDNPSNLLAYGPIYLRCSELLAGL
                     GVIDAAGPTSFALVGRDGGQHDVTIEPIPAEDDVAWNSGQPHDLPPTDALWLKDQGKT
                     LWWTYLEDSRTLFVQFNTVERGTSRLTEGILARAKQTDVARVVVDLRHNGGGDNTTLG
                     PLDFALRDPAIDRPGRLFVIIGRITFSAAANFATDLEQETGAIFAGEDMGGSPNMYGD
                     AHRIGLPYGGQAVYMATRYWERSTPDDRRITIEPEIHADLSSADYIAGRDPVLQAILD
                     TPVAPG"
     gene            complement(3497..3970)
                     /locus_tag="VHM48_01345"
     CDS             complement(3497..3970)
                     /locus_tag="VHM48_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2754073.1"
                     /translation="MIRRDDSRLSLLFEVYAASRAVGELLAAAMADSPLTPEEYAVYS
                     VLFDEGPHAPTELARRTGMPPTSMSHFVRSMLERGHAQRAPSPNDHRSYRIVLTAAGL
                     TAHAASSTAFAEADERFIRALAADEEDARATLRAIGRAATVGGERLAADAVADTA"
     gene            complement(4189..4371)
                     /locus_tag="VHM48_01350"
     CDS             complement(4189..4371)
                     /locus_tag="VHM48_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754074.1"
                     /translation="MQLATAAASLVVGGVVGAVDWSRYTDLGRVIGAELMFLALAIWI
                     FRRGRHSLRRADEPTA"
     gene            complement(4410..4835)
                     /locus_tag="VHM48_01355"
     CDS             complement(4410..4835)
                     /locus_tag="VHM48_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX2754075.1"
                     /translation="MTTLQIEVGDLHFSARWEAAAPHTIEAIRRMLPIRSKLIHCRWS
                     GESTWIPYGDFRPGIDYENHTSHPAPGMLAMYPGGISECEIFFPYGACQTASKVGPLA
                     ANHFASIVPDDGWADRLREVGRRCLWDGAQEIVISEVDG"
     gene            4851..5189
                     /gene="uraH"
                     /locus_tag="VHM48_01360"
     CDS             4851..5189
                     /gene="uraH"
                     /locus_tag="VHM48_01360"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX2754076.1"
                     /translation="MADGTGPTISTHVLDTERGLPAEGVRVRLFRLDVPNAPIRMTQA
                     LTDGDGRVRDLLERPLVAGDYRLEFDFGREGDEFFRRIALDLRIADPTRSYHVPLLLA
                     PYSMTTYRGS"
     gene            5190..>5381
                     /locus_tag="VHM48_01365"
     CDS             5190..>5381
                     /locus_tag="VHM48_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754077.1"
                     /translation="MDVAALAALDELPTDAFVAAAAPWFEGAPRFLARLARARPFGDE
                     STFFARAAEIALAMPEAEQV"
BASE COUNT          774 a         1869 c         1979 g          759 t
ORIGIN      
        1 cctggcggag cacgatcgga tcggatcgcc gctccggagc gagctcggac ccggtgaacc
       61 gcccgagcgc gtcgaggaga ccctccgcgc aatcggtctc acggcgccgg gcgagctcgt
      121 cgagctgttc gggtggcgcc aggtccggcc cgagctgctg ggtgcgccgc gcgtcgcctg
      181 gttctggccg gcccagccgc gccatttcga cgaagcggtc gaccactacc gccagtcgat
      241 cgagatcggc ggcatcaccc cggccgcgct cgagaaggcg atccgggcgg gccaaccgcc
      301 cgaggcgacc ttcaccgggt tctggcggac ggactggttt ccgtggctct tcgggagccc
      361 ggaggaatat gcgatcgaat gccgcgacgg cgcgtcggag gcgcccgtct ggcgggtcaa
      421 ctggcatccg gaccagggct tccagacgaa gcaggtcggg acgagcgtga cggacttcat
      481 cgagcgggtc gtggagctgt ttcgggccgg tgcgtacacc tgggacccgg cggaccactc
      541 catcgttccg gtcgacgagg tgttcacgca acgaggtctt gacgagaatc ggccgccatg
      601 ggaccgccat taccggccgg cctcgctccg ttcgacgaac tgaccgtacc ctcgctcgcc
      661 ggccgcgaca ccgtcgcgaa tgaccgcccg gccgcggacg aacacggacc ggactgcgcc
      721 ggcgacatcc atcccctcgt acggggtgaa gtcactcgcg tgatggagat cggcggcccg
      781 gatcgtccgt cgggcggcag ggtcgaacag cacgaggtcg gcatcgcgcc cgacctcgat
      841 cgcgcccttg cgggcaaagc cgaaccgtcg ggccggggtc gtcgcgacga ggtcgaccat
      901 ccgctcgagg gtgatccgcc cgccggcgac accgaccccg tacgccaggg tcagcagggt
      961 ctcgatgccg ggcgccccgt tgctgagctg gtcgaacgac acgccgcgtg ccgcctcacc
     1021 cttctcgacg gcgacccggt ccgggacgtg gtccgtcgcg atcatcgaga gatctccggc
     1081 ggcgaggccc gcccagagcg cgtcgcgatc ggccgccggg cggaggggcg gggtgaccag
     1141 gctcttggca cattcgaccg ggtcgcgctc cgcgtatcgc tcatcggtca gggtcaggta
     1201 gtggggacac gtctcggcgg tcacccggac gccgtccgcc cgcgcctgcg cgacgaacgt
     1261 gagcgcggcg gcgcacgaga ggtggacgac gtggatcggg gccccggtgg cccgggcgaa
     1321 ggccatcacc cgatgggtgg cgaccgcctc ggtctcggtc gttcgcgtgg cagcgtggtg
     1381 ccatggtgac gtgtcaccac gctgaagcgc cgcggcgacg gcggcatcga cgaggacggg
     1441 gtcctcgcaa tggacctcga gcaggccgcc ccgggcgccg aggatcgcca tcgcgtcgtg
     1501 gatcgcgcgg tcgccgagcc ggaagtcgaa gatcatgaag gccttggccg tcggcacgcc
     1561 ggcatcgacc atcgccggca gctccgtgag tggatcgtcc atcccgccga ggatcgcggg
     1621 gctcagggcg tagtcgaccg cggcgtcgga cgcggtcacc cgccggaact cctcgatccc
     1681 ggccagaatc gagcgatgcg ccgccgccga cgagccggtg ccggggttgt tgaacgcgag
     1741 gaacgtcgtc gtcccgccga atgccgcggc tagagagtcc ttgaagaagc ggtcgggctc
     1801 ggcgtcggtc ccgacgcggg tgtgggtgtg gacgtcgacc gcgcccggca ggatgaggag
     1861 cccggacgcg tcgacgatct cgcggctccc cgccgccaga cccgacaggt cggtttcgat
     1921 cgtctcgatg gcgcccgtcc ggaccgcgac atcggcccgc cgcgaccccg cggcggtgac
     1981 gacggtcccg cccaggacga gcaggtccgg cccgagtccc tcgatcgaca tcgtcacccg
     2041 tcgaggatag ccagcgcgtg accccggcgg gacccaggcg cagggcgggc aaacagccgt
     2101 cgcccccgct ggacgtcgtc agccgggcgc gactggtgtg tcgaggatgg cctgcaacac
     2161 cggatcacgg ccggcgatgt agtcggcgga cgacaggtcg gcgtggatct ccggttcgat
     2221 cgtgatccgc cggtcgtcgg gcgtgctccg ctcccagtag cgggtcgcca tgtagaccgc
     2281 ctggccgccg tacggcaggc cgatccggtg ggcgtcgccg tacatgttcg ggctgccgcc
     2341 catgtcctcg ccggcgaaga tcgcgccggt ctcctgctcg agatcggtcg cgaagttggc
     2401 ggccgcggag aacgtgatcc ggccgatgat cacgaacagc cggcccggcc ggtcgatcgc
     2461 cgggtcgcgg agggcgaaat cgaggggacc cagggtcgtg ttgtcgcccc cgccgttgtg
     2521 gcgcaggtcg acgacgaccc gcgcgacgtc ggtctgcttc gcccgagcga ggatcccctc
     2581 ggtgagccgg ctcgtgcccc gctcgaccgt gttgaactga acgaacaacg ttcgtgaatc
     2641 ctcgaggtac gtccaccaca gggtcttgcc ctggtctttg agccagagcg cgtcggtcgg
     2701 cggcaggtca tgcggctggc cggagttcca ggcgacgtcg tcctcggccg ggatcggttc
     2761 gatcgtcacg tcgtgctgcc cgccgtcgcg accgacaagc gcgaacgagg tcggtccggc
     2821 cgcgtcgatc acgccgagcc cggcgagcag ctcgctgcaa cgcaggtaga tcggtccgta
     2881 ggcgagcagg tttgacgggt tgtcgcgcgg cgcgaggggc tcgacgaggg cgagcacatc
     2941 ggcgatcgga cggccgttga ccgcctcgac ccgcgagccg acgagatcct catacggcgc
     3001 ccgcgcggcc gtgatcacga ggccatcgct gaaccgccac cagcggaccg ggtactcgtg
     3061 cgtcccgctg ccggggatga acgggaagat cccgctgtgg ccatcgcgac cgttccagct
     3121 cggcatcgcg gcgagccgga cgagctcgac gagggtttgg tcgtcggtca gcctcgggat
     3181 cctggccttg acggcgtccg ccgcggcaac ccggtcggtg cgcttcatcc cgtgccagcc
     3241 gtcgggatgg acgcggtcgc gggtgtcgag cagggcgtcg atgtcggccc gccagccggc
     3301 ggcccggtcg aggacgtcgc tggcggatgg cgcggcggtg gcggacggcg gcggcgcggc
     3361 gatcgacccg ctcggcgggg acgacgacgg ggcggttgac gccgacggga ccgccggcga
     3421 cgtgccacac ccggtcgcga gcgcgacgac cagggcccac agcacgatcc tgccagatcg
     3481 cagcatcatg gccggctcac gccgtgtccg cgaccgcatc ggcggcgagg cgctcgccgc
     3541 cgaccgtggc ggcccgcccg atcgcgcgaa gggtcgcccg ggcatcctcc tcgtcggcgg
     3601 ccagggcccg gatgaaccgc tcgtcggcct cggcgaacgc cgtgctcgag gcggcgtggg
     3661 cggtgagacc ggccgccgtc aggacgatcc ggtacgagcg gtgatcgttt ggcgagggcg
     3721 cccgctgcgc atggccgcgc tcgagcatcg accggacaaa gtgcgacatc gacgttggcg
     3781 gcatgccggt gcgcctggcg agctcggtcg gggcatgggg tccctcgtcg aagaggacgc
     3841 tgtagaccgc gtactcctcg ggcgtcagcg ggctgtcggc catcgctgcc gccaggagct
     3901 caccgacggc gcgcgaggcg gcgtagacct cgaacaggag ggagagcctg gagtcatcgc
     3961 gtcggatcac gggacgatac taccagatgg cagtagctca ctggtgacgc gacgccatgt
     4021 gcacgccagg cgggcattgc gacgaacgag ccgcccggat cggctgttcc ttcgccgact
     4081 tgcacgcccc gtaggcagga cggcgtcgga gcggcccgtg tcgatcgttc cttcgtcgca
     4141 acgcccactg ggcgtgcagt cggcagaggc gccgcgccgc gccgcgcctc aggcggtcgg
     4201 ctcgtccgcg cgccgcaggc tgtgccgtcc gcgccggaat atccagatcg cgagagccag
     4261 gaacatcagc tcggctccga tcacccgacc gagatcggtg tagcgcgacc agtcgactgc
     4321 gcccacgacg ccaccgacga ccagcgaggc ggcggccgtc gcgagctgca cgagcgcagt
     4381 gttgcgagtg cggatgcggt gcgcagcatt catccgtcga cctcgctgat cacgatctcc
     4441 tgggcgccgt cccagaggca gcgccggccg acctcacgca aacggtcggc ccagccgtcg
     4501 tccggcacga tggaggcgaa atggttcgcc gcgagcggcc cgaccttcga cgcggtctgg
     4561 cacgcgccgt acgggaagaa gatctcgcat tcgctgatcc cgcccgggta catggcgagc
     4621 atgccggggg cgggatgcga ggtgtggttc tcgtagtcga tgccgggccg gaagtcgcca
     4681 tacgggatcc aggtcgactc gccgctccag cggcaatgga tcagcttcga gcggatcggc
     4741 agcatccgcc ggatcgcctc gatcgtgtgg ggcgcggcgg cctcccagcg ggcgctaaag
     4801 tggaggtcgc cgacctcgat ctggagcgtc gtcatgagcg gaggataacg gtggccgacg
     4861 gcaccggacc gacgatctcg acccacgtgc tcgataccga gcgcgggctg ccggccgagg
     4921 gcgtccgggt gcgcctgttc cggctcgacg tcccgaacgc cccgatccgg atgacccagg
     4981 ccctgaccga cggcgacggc cgcgtccgcg acctgctcga gcgaccgctc gtcgccggcg
     5041 actatcggct cgagttcgac ttcggccgcg agggcgacga gttcttccgg cggatcgcgc
     5101 tcgatctccg gatcgccgac ccgacgcgga gctatcacgt cccgctgctc ctggcgccct
     5161 actcgatgac gacgtaccgg ggcagctgag tggacgtcgc ggcgctcgcg gcgctcgacg
     5221 agttgcccac ggacgcgttc gttgccgccg cggcgccgtg gttcgagggg gcgccgcggt
     5281 tcctcgccag gctcgcccgc gcgcgaccgt tcggcgacga gtcgacgttc tttgcccgcg
     5341 cggcggagat cgcgctcgcc atgccggagg ccgagcaagt c
//
LOCUS       DATAIW010000038         5859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1275, whole genome shotgun sequence.
ACCESSION   DATAIW010000038 DATAIW010000000
VERSION     DATAIW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VHM48_01370"
     CDS             complement(<1..378)
                     /locus_tag="VHM48_01370"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754078.1"
                     /translation="MTGDGGSPAVAPAPVGGALDVFRNRPFLLLWLAQAATQIGGNMV
                     IFGLTVIISKSTGSTTAVSALILTFLLPAVLFSALAGVFVDRLDRRLVLILTNVLRGL
                     AFIGMFFVGTNLVLIYLLNIIVST"
     gene            complement(375..620)
                     /locus_tag="VHM48_01375"
     CDS             complement(375..620)
                     /locus_tag="VHM48_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX2754079.1"
                     /translation="MTDRPALTLYLGDLVRLRRVHPCGGDTWLVDRLGADIGLRCQTC
                     QRHVLVERRLIERRLVEFVRRGDPILSAAAAPRPSAP"
     gene            complement(634..1311)
                     /locus_tag="VHM48_01380"
     CDS             complement(634..1311)
                     /locus_tag="VHM48_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2754080.1"
                     /translation="MRDLRSQLTIAAVAAILGLLVVVQLRGQTGGSELQSKSAQELSS
                     IVANMNDENDRLRAEIATLQNQLAELRTDRANGATSVDQLVSELGRIRAWAGLDPIAG
                     RGVKITVNGEIDAAAVDDLLNELRNAGAEAIAIEDLRVVARTAVSGVPGSLDVDGFLL
                     RDPFTIRAIGKPETLVGSLTRVGGIIAQLSATNPAATVDVQAVDDPMTLPATKRNLVP
                     DHGHPGL"
     gene            complement(1320..2111)
                     /locus_tag="VHM48_01385"
     CDS             complement(1320..2111)
                     /locus_tag="VHM48_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2754081.1"
                     /translation="MTTIGARIRAIPSWQVTLGIALLALGFLIAAQLAAEGPRVRYTT
                     QERSPLVETTLALQAQRADLTSRIVALRKQIQDIEQPGTGSAAVLRDLNARLEEARIA
                     AGLIALTGTGIVLKLEDSTEPVPPDANEADYLAGAADLRTVVALLWQSGAEAIAINGE
                     RIASSTAIIDIGGSVLINAAYVAGPYQIFAIGPKELFARLSAAPGWGEFVRTRRGSFG
                     IGISWAEPETVDVPAFAGSVTLRESRSVPSPTPPAAPSRPAGTAP"
     gene            complement(2108..3784)
                     /locus_tag="VHM48_01390"
     CDS             complement(2108..3784)
                     /locus_tag="VHM48_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX2754082.1"
                     /translation="MGPLSVSRGRRTGVLVVVAIWALLGASASVAVAADPVTFGDPAA
                     TSKFGEAIEFKQPVTINGTPQRVEILIDTPGSIGPSVQTVPEGASAGASTLDYRLDLT
                     GGSVVPNTTFTARWRVTDESGRTWLGPSIRHTYADDRFDWKTLNGDVVRVHWVEGDQA
                     FGKRALKIGDDAVAATAKLLGVTESEPIDFFIYADQQSFYDALGPATRESVGGQAHPD
                     IRTLFALITPDEINASWVESVVPHELTHVVFQTAVDNPYHDPPHWLNEGLAVYLSDGY
                     GDFDRSQVKQAAADGSIIPLDGLAGAFPTTTDRFFLAYAESVSAVDRMVRIDGRDALV
                     KLIRSYHDGVSDDEAFRAALGSDVAGFEADWLAELGASPPTRLGPRPAPVGPLPSGWN
                     GPQPNPSFEIVGSQPPVTPGAPVPGPRGGGSDAISFLVAPLATIGLVVIVVILAVGLR
                     RLGRRPDPRTWAETGDNRLFWRPPTGEPPADDTAPPSRLWSSTDWSLPRDPAETVDTA
                     RPDLDPSRGDTPGDRAAPEMLAAEPGDVSTPTDRDGTQESSASGDPSTGT"
     gene            complement(3772..5391)
                     /locus_tag="VHM48_01395"
     CDS             complement(3772..5391)
                     /locus_tag="VHM48_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2754083.1"
                     /translation="MVVASDGLTRPDGRARRVRLGLVAVLIVASATLVGPVVPRPGAP
                     AVRAADTDTVRILIGAADNLDPAAQGDIGSAAISAQLFESLTAIDAQLQTRPALASSW
                     DFRDGGATVVFHLRPGITFSDGSPITAADVVRSWFRIIHPEHPSPLVSLIGDVRGALE
                     FARGNGERDVVGLAADGNDVIVHLTRPATDFPTIVAGPTFAIVPASLDQGPQALEPGD
                     GFVGSGGYVLTAATGTKTTLSANDRYWAGRPAIGTVELIHDIGGRSSVTAFEADDVDL
                     TDVSPFDATWLAYDDTLGPLLRRTDSLSLEYYGFDTSRPPFDDVRVRQAFARAVDWRR
                     LIALAAAGAATPATGMVPPGIAGRSVTDFLPALDPDGARKLLADAGFPGGSGFPTVTL
                     LGGGATDRAFAAEIKRELGVTVQVELEGAGFFDRLAADPPQMFSMGWIADYPGPNDFL
                     GILLGGGASNNYGRWTSSAFDGAVADALAATDPAAARAAFDRAEGIVRDEAPTIPLTY
                     DVSWSLARSGLLGAYENGLGIIRMAGLAWDR"
     gene            complement(5367..5540)
                     /locus_tag="VHM48_01400"
     CDS             complement(5367..5540)
                     /locus_tag="VHM48_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754084.1"
                     /translation="MRRWVCIVALLAGFTRDDYVRGIRLREPVAVERLIETRRRGTRG
                     LVPTGAWWSRPTG"
BASE COUNT          896 a         1973 c         2186 g          804 t
ORIGIN      
        1 cgtcgagacg atgatgttca ggaggtagat caggacgagg ttcgtcccga cgaagaacat
       61 gccgatgaac gccagcccgc gcaggacgtt cgtgaggatc aggaccaggc gccggtcgag
      121 gcggtcgacg aagaccccgg caagggcgga gaacaggacc gccggcagga ggaacgtcag
      181 gatcagcgcg ctcaccgccg ttgtcgaccc ggtcgacttc gagatgatga ccgtgaggcc
      241 gaagatcacc atgtttcccc cgatctgggt cgccgcctgg gcgagccaga ggaggaggaa
      301 cggccggttg cggaagacgt cgagcgcacc gccgaccgga gcgggcgcga ccgccggcga
      361 tcccccgtcg cccgtcacgg ggcgcttggt cgcggcgcgg ccgcggcgct caggatcggg
      421 tcaccgcgcc gcacgaattc gaccaggcgg cgttcgatca ggcggcgctc gaccaggacg
      481 tgccgttggc acgtctgaca gcgcagcccg atgtccgcgc caagccgatc gacgagccag
      541 gtgtcgccac cgcacggatg gacccgtcga aggcggacga ggtccccgag gtacagcgtg
      601 agcgccgggc ggtcggtcat ccggcggagg gtatcaaaga ccgggatgac catggtccgg
      661 aacgaggttt cgcttcgttg ccggcaaggt catggggtcg tcgaccgcct ggacgtcgac
      721 ggtcgcggcc gggttggttg ccgacagctg ggcgatgatc ccgccgaccc gggtcagcga
      781 gcccacgagc gtctcgggct tgccgatcgc ccggatcgtg aacgggtcgc ggagcaggaa
      841 cccgtcgacg tcgagcgacc ccggaacgcc gctcacggcc gtccgggcga ccacccggag
      901 gtcctcgatc gcgatcgcct cggcgccggc gttgcgcagc tcgttgagca gatcgtcgac
      961 ggccgcggca tcgatctcgc cgttgaccgt gatcttcacg ccgcgtccgg cgatcggatc
     1021 gagacccgcc cacgcccgga tccggccgag ctccgacacg agctgatcga ccgaggtggc
     1081 gccgttcgcc cgatcggtcc ggagctcggc cagctggttc tggagcgtcg cgatctccgc
     1141 ccggaggcga tcgttctcgt cgttcatgtt ggcgacgatc gagctgagct cctgggccga
     1201 tttcgactgg agctcggatc cgccggtctg accgcggagc tggaccacga ccaggaggcc
     1261 caggatcgcg gcaacggcgg cgatcgtgag ctggctgcgg agatcgcgca tcgccggctt
     1321 cacggcgcgg tcccggcggg ccggctcggg gcggctgggg gcgtcgggct cggaacggaa
     1381 cgtgactcgc gcagggtgac cgagccggcg aaggccggaa cgtcgaccgt ttccggctcg
     1441 gcccacgaga ttccgatgcc gaacgagccg cgccgtgtcc ggacgaattc cccccagccg
     1501 ggcgcggccg acaaccgagc gaacagttcc ttggggccga tggcgaagat ctggtaggga
     1561 cccgccacgt aggccgcgtt gatcaggacc gatccgccga tgtcgatgat cgcggtcgag
     1621 gaggcgatcc gttcgccgtt gatcgcgatc gcctccgccc cggactgcca gagcagggcg
     1681 accacggtcc gcagatccgc ggcgccggcg agatagtcgg cctcgttggc gtctggcggg
     1741 accggctcgg tcgagtcctc gagcttgagg acgattccgg tgccggtgag cgcgatcaat
     1801 ccggcggcga tcctggcttc ctcgagtcgc gcattcagat cgcggagcac cgccgcggaa
     1861 ccggtccccg gctgctcgat gtcctggatc tgcttgcgca gggcgacgat ccgggacgtg
     1921 agatcggccc gctgagcctg cagggcaagc gtcgtctcca cgaggggcga gcgttcctgg
     1981 gtcgtgtacc ggacgcgcgg gccctcggcc gccagctggg ccgcgatgag gaagcccagg
     2041 gcgagcaggg cgatgccgag cgtgacctgc cacgatggaa tcgcccggat ccgggcgccg
     2101 atggtcgtca cgtcccggtc gatgggtcgc ccgatgcgga cgactcctgg gtgccgtccc
     2161 gatcggtcgg ggtcgagaca tcgcccggtt cggcagctag catctccggc gcggctcgat
     2221 cgcccggggt gtcgccgcgg gatgggtcga gatcgggccg ggccgtgtcg acggtctcgg
     2281 ccgggtcccg tggcagcgac cagtcggtcg aggaccacag tcgcgacgga ggcgccgtgt
     2341 catcggccgg tggctcgccg gtcggcggcc gccagaacag ccggttgtcg ccggtctcgg
     2401 cccaggtccg cgggtccggc cggcggccga gccggcggag cccgacggcg aggatcacga
     2461 cgatgaccac gaggccgatc gtcgcgagtg gagcgacgag gaagctgatc gcgtccgacc
     2521 cgccgccgcg gggtcccggg acgggtgcgc cgggcgtcac cggcggctgc gacccgacga
     2581 tctcgaagga cgggttcggc tgcggtccat tccagcccga gggcagcgga ccgaccggag
     2641 ccggtctcgg gccgaggcgg gtcggcggac ttgccccgag ctcagcgagc cagtcggcct
     2701 cgaaccccgc gacgtcgctt ccgagcgccg cccggaatgc ctcgtcgtcc gagacgccgt
     2761 cgtggtacga gcggatcagc ttgacgaggg catcgcgacc atcgatccgg accatccggt
     2821 cgaccgccga aacgctctcg gcataggcga ggaagaaccg gtccgtggtg gtcgggaagg
     2881 caccggcgag gccgtcgagg gggatgatcg aaccatcggc cgccgcctgc ttgacctgcg
     2941 agcgatcgaa atcgccataa ccgtccgaga ggtagacggc gaggccctcg ttcagccagt
     3001 ggggcgggtc gtgatacggg ttgtcgaccg cggtctggaa gacgacgtgg gtcagctcgt
     3061 gggggacgac gctctcgacc cagctggcgt tgatctcgtc gggcgtgatc agggcgaaga
     3121 gcgtccggat gtccgggtgc gcctggccgc cgacgctctc gcgggtcgcc ggtccgagcg
     3181 catcgtagaa cgactgctgg tcggcataga tgaagaagtc gatcggctcc gattcggtga
     3241 cgccgaggag cttggccgtg gcggccacgg cgtcgtcgcc gatcttcagg gcccgcttgc
     3301 cgaaggcctg gtcgccctcg acccagtgga cccggaccac gtcgccgttc agcgtcttcc
     3361 agtcgaagcg atcgtcggcg taggtgtggc ggatcgacgg gccgagccac gtccggccgg
     3421 attcatcggt cacccgccag cgggccgtga acgtcgtgtt cggcacgacg ctgccgccgg
     3481 tcaggtcgag gcggtagtcg agcgtcgagg cgccggccga cgcgccctcc ggcacggtct
     3541 gcacgctcgg cccgatcgaa cccggcgtgt cgatcaggat ctcgacccgc tgcggcgtgc
     3601 cgttgatcgt gaccggctgc ttgaactcga tcgcctcgcc gaatttcgag gtggcagccg
     3661 gatcgccgaa ggtgaccggg tcggcggcga ccgcgaccga tgcgctcgca ccgagcaggg
     3721 cccagatcgc gaccacgacg aggacgccgg ttcgccggcc gcgtgacacg ctcagcggtc
     3781 ccatgcgagt cctgccatcc ggatgatccc gaggccgttc tcgtaggcgc cgagcaggcc
     3841 gctccgggcc agcgaccagc tgacgtcgta ggtcagcggg atcgtcggcg cctcgtcgcg
     3901 gacgatgccc tcggcccggt cgaacgccgc acgggccgcg gccggatcgg tggccgcgag
     3961 cgcgtcggcc accgccccgt cgaacgcgct cgacgtccag cggccgtagt tgttcgaggc
     4021 gccgccaccg aggaggatgc cgaggaagtc gttcggaccg ggatagtcgg cgatccagcc
     4081 catcgagaac atctgcggcg ggtccgcggc gagacggtcg aagaagccgg cgccctcgag
     4141 ctcgacctgg acggtcaccc cgagctcgcg cttgatctcg gccgcgaacg cccggtccgt
     4201 ggccccgccg ccgaggagcg tcacggtcgg gaatccggag ccgccgggga acccggcatc
     4261 ggcgaggagc ttgcgggcgc cgtccggatc gagcgcgggc aggaagtcgg tcacgctccg
     4321 gcccgcgatt ccgggcggaa ccatgcccgt cgccggcgtg gccgcaccgg cggcggcgag
     4381 ggcgatcagg cgccgccagt cgacggcccg ggcgaaggcc tgccggacgc ggacatcgtc
     4441 gaacggcggg cgcgacgtat cgaagccgta gtactcgagc gacaagctgt cggtccggcg
     4501 gaggagcggt ccgagcgtgt catcgtaggc gagccacgtc gcatcgaacg gcgagacgtc
     4561 ggtcaggtcg acgtcgtccg cctcgaacgc ggtcaccgag cttcgcccgc cgatgtcgtg
     4621 gatcagctcg acggtcccga tcgccgggcg gccggcccag tagcgatcgt tcgcgctcag
     4681 cgtcgtcttc gtccccgttg ccgcggtcag cacgtagccg ccactgccga cgaacccgtc
     4741 gccgggctcg agggcctgcg gcccctgatc gagcgaggcg ggcacgatcg cgaacgtcgg
     4801 cccggcaacg atcgtcggga aatcggtcgc cggccgggtc aggtggacga tcacgtcgtt
     4861 gccgtccgcg gccagcccga cgacgtcgcg ctcaccgttg ccgcgggcga actcgagcgc
     4921 accgcgaacg tcgccgatca ggctgacgag cggcgatgga tgctccggat ggatgatccg
     4981 gaaccagctc cggacgacgt cagcggccgt gatcggagag ccatcgctga acgtgatccc
     5041 gggtcgaagg tggaagacaa ccgtcgcccc gccatcgcgg aagtcccacg acgacgcgag
     5101 agcgggccgg gtctggagct gggcgtcgat cgcggtcagg ctctcgaaca gctgggccga
     5161 gatcgcggcg ctgccgatgt cgccctgggc ggcagggtcc aggttgtctg ccgcgccgat
     5221 gaggatccgg acggtatccg tgtcggcagc cctgacggct ggcgcgccag gccgcggcac
     5281 caccgggccg acgagggtcg ccgacgcgac gatcaggacg gcgacgaggc cgaggcggac
     5341 ccgccgagcc ctgccgtcgg gtcgagtcag cccgtcggac gcgaccacca cgcccccgtg
     5401 ggtacgagac cgcgcgtccc gcgtcggcgg gtctcgatca ggcgttcgac ggcaacgggc
     5461 tcgcgcaggc ggatcccgcg gacgtagtcg tctcgggtga agccggcgag gagggcgacg
     5521 atgcagaccc agcggcgcat ggccggagta taggggcggg tcggcgagag gcccggtccg
     5581 tcgctgggcc ggcccgagcg cccgtgcggc gacacccacg gagcctgggc gccgtcgagc
     5641 gccgggcagg aattgaggcc tcgcaagtca caccgccggg ggaacgatgc attgccgcga
     5701 ggcctctgtc cggataacga tgcgtcgtcg gaaacgttag ggcgattcga gggaagatcg
     5761 tggaacatcg acggcgagca gccagaacct tggtgaagac gacgaatttc gcatgaccct
     5821 ccgcctgcgc aacaccctga cccggtccgt cgagcccgt
//
LOCUS       DATAIW010000039        14160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_119750, whole genome shotgun sequence.
ACCESSION   DATAIW010000039 DATAIW010000000
VERSION     DATAIW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119750"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VHM48_01405"
     CDS             <1..312
                     /locus_tag="VHM48_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754085.1"
                     /translation="NRSSPGARLASVLAHSDSAARIGRAALAGGFVERDVRGLLAGLA
                     GSIPDLDALLRDGGDDDVRAALDVARRREFAAPGAAVLRIDGWIVAPTEARACALIAL
                     A"
     gene            complement(330..1952)
                     /locus_tag="VHM48_01410"
     CDS             complement(330..1952)
                     /locus_tag="VHM48_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2754086.1"
                     /translation="MPHPTSDAAINLTPRQKLEILGAILLAMFLFALDQTVVGTALPR
                     IISALHGETLYTWSVTIYLLTSTISGPIYGKLSDLFGRRPIVIFAVSLFLISSALAGL
                     SQNMEMFILFRGLQGLGGGAVFPIALAVVADLYTPAERGKYLGLFGAVFGVSSLVGPG
                     LGGFITDTLSWNWIFYINIPIGLVSLFILWRLLPAIKRPDAAEHIDYVGAAVFTAAIA
                     PFLVGLSNKQTGQWTDPAVGGLMLVGLVFGAIFLWVETRAVDPIVPLDLFRLRTFSIS
                     VASMFLAAFGFFTAVIFLPRWFQTVGGASATESGYNLLPLLAGLIFSAIASGQIVARV
                     GRYKILMAGSLALLALALLLFTNLRADTDRPVMWLWMALAGLGIGPSFAVFTLIVQNA
                     VSPDRIGVATSSLTFFQQIGGTVGLTLAGTLLADKITTELPAQIVKAGVPPAFLTGFS
                     PDSPVATRLDLFGTGDLAGRLATVIPPEGQGLIPAIVKGVHEAFSIAIASTFWIGIIG
                     GAVAAVLVLFLHEVPMRQTFEVEDYAPEHART"
     gene            2350..2499
                     /locus_tag="VHM48_01415"
     CDS             2350..2499
                     /locus_tag="VHM48_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754087.1"
                     /translation="MAGVRPVLGAPVFGSMISGWYGINRSSTAADYLAAARRHPAFEL
                     RPVRS"
     gene            complement(2489..3085)
                     /locus_tag="VHM48_01420"
     CDS             complement(2489..3085)
                     /locus_tag="VHM48_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754088.1"
                     /translation="MIRIRTLITGGLSSTILIGALAGGTFAAPERIPVSSVDDQSLSI
                     SNRIAAVDHVVLAMGGRLDRIVASLPADHPPVPILGGLISTRSTIGTLIETIDAQVCN
                     QDGVTGTGDSSLADADAFAQDSSSTGLLNQLSSVRGVLAEGRGRLIRIAGAYPAGPPV
                     DEVRNALLTVRSDAVIAFETVNVRIDDGNHPPSPCVTT"
     gene            complement(3082..3393)
                     /locus_tag="VHM48_01425"
     CDS             complement(3082..3393)
                     /locus_tag="VHM48_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX2754089.1"
                     /translation="MTYLIEGYWPPSAGRADPERLARAAEASAHGDVRYLGAVTVPDD
                     ATVFWLFDAPSIDALRAAAAAAGLTADRISEATAQDLGGLPTAVPPHHHLVARTKGAN
                     S"
     gene            complement(3467..5617)
                     /locus_tag="VHM48_01430"
     CDS             complement(3467..5617)
                     /locus_tag="VHM48_01430"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX2754090.1"
                     /translation="MIRNRLRQRLAVLAGGMLLVLGSVGSATAAPQTRTIGLAYQGET
                     TSDLGYDFDVSGVCDDCVPGVFETLLGGGRWAYGATATGRVNHVAWQAATSTALNYDD
                     ALLRQGQTLGLSDVLTPTGGAIVATGKIEGSAGLLVDPTGGTNFGPSGASDDIDKNAT
                     WTASCAMPLPGDGARLCVSDTQDIQLASRAVAGTVLTGTLNLVLSIQLRLEFTVSSDG
                     IIATRAVTFTGGSGSLNRSLAFVGATPSTLADPIALSCTQPSGADVVYSLQADPYAPT
                     TNVAGRGTLHAAAAIKFPLLDPIEVVGSDITSITAPTHAVPLALTGNAGSATLGALAK
                     NNIPPTPDSGGGASHEYSGDQGTPITFDGSASSSPCGFPTLRWDFSDGGVAFGAKPKH
                     TFQGSGTYSGLLTATDVTGLTATQTFSVEIFNQAPLVDAGLDTIAAWGRSVAFTGQAV
                     DPGTDDQSTLTYWWRFGDGTPDATGSGPSVTHAYAAPSPVNEPYTAMLTVCDRHGACQ
                     FDTRLVTIRARDVSVGAVGDTAATFDTGARLRASLVDEFGQAVNGRTVSFAVETVPGA
                     SGSAVTSSTGLASTAWTPGLAAGSYGITASFAGDTFYSAASGGGNVSIARKATTTAYT
                     GTLTGGANKTVTLSATLVDATGTPLAGRTIAFKLGAQTTSAVTSASGVASVQLKLTQK
                     NGTYPLTATWTPAGSDAARYLPSAASATFKLQAK"
     gene            5763..8570
                     /locus_tag="VHM48_01435"
     CDS             5763..8570
                     /locus_tag="VHM48_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2754091.1"
                     /translation="MVDVLGRITEQAALDRFVTEARTGARGLAIEGEAGIGKSTLWAS
                     AVAAARSGPGQASAGLDPAAPPAVAIVLEARPASPEVRLSFSGLVDLFEPVADTVLGA
                     LPGPQRQALEVALYRSPAGDSSESGLVGVAVLTALRALARQRPVVVAIDDLQWLDAAT
                     SIALQHAVRRLRSEPIGLLTATRTGPDVEPAVDLVGLLAEDAAERLHLGPLPMGAIHH
                     LIRTRLDVSLPRPTLVRLHADAGGNPLYALELARHAASAPDHFASDDKARVPSPLADL
                     LRQRLAGLAEPTRAILLVVALAGGATLDEIARAAGIPIGDARARLEPARRDGLLERSG
                     NELRISHPLIAAAIADSAEPDARRSVHRRLAELADEPETRARHLAAGASQPDPAVAAA
                     LSEAAEQALARGAIDASVELLDEALRHWPADDGPGRRPLRVKLAERTFLAGDTARAHR
                     DLEALLPELVEPDLRVHAAILLGTIVFYDGDSRVASQILLDELVRTDDRVGRGRIHAR
                     LSWTLEHDLAAAAEHSRLAVETLDPAEAPDIYSFALMNLVVVELELGRGADHEAMERG
                     HQMQERAQSWDHSSLPANWAKWFDDFERSRVLNHLYLDRGRQTGDESSVAQLLSYLVE
                     LEAWTGHMDLAEVVANDAVAAAEQTGQPSYLSAALARRAFVSASLGRLADARVEATRA
                     TQLGVELSSPLLEALGLGVLGFIELSAGAPEVAARHTDRALLKLDETGMFDHPMFRFH
                     ADQIEAHVRIGELDVAERLLDRQRDRQRRAPRPWLALTAARTGALLAAARGDVPGAVA
                     LVDEALAIAPTLAWPMEIGRTWLVAAEIRRRAGQRKAAGAAVAEAIGRFESIGAAAWL
                     ARARRDAAALGIDPSEHPDSLTPSEDRVARLAAGGMTNREIAARLAISPKTVEASLAR
                     TYHKLGIRRRAELATRLARET"
     gene            8727..9605
                     /locus_tag="VHM48_01440"
     CDS             8727..9605
                     /locus_tag="VHM48_01440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2754092.1"
                     /translation="MKLGLQIPSFTWPGGTPAIGPTLARTARLADDIGLDSIWVMDHF
                     FQIRGVGPVEQAMLEGWTALGFLAANTSRARLGLMVGGVHYRLPGLWVKAATTLDVLS
                     GGRAWLGIGAAWNEDESRSLGFPFPPLGERFEMLEETLRIAHEMWQGERGSEAAFDGR
                     QFHATRLMNSPQSISRPRVPIMIGGGGEKKTLRFVAQYADATNVFGGPEAIAHKYEIL
                     REHCAAVGRDYDEIERSNLQTINFGRETPSQVVERFGELADAGAQHVIFSVADVHDPV
                     HLETIGREILPQLRDL"
     gene            complement(9613..10428)
                     /locus_tag="VHM48_01445"
     CDS             complement(9613..10428)
                     /locus_tag="VHM48_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754093.1"
                     /translation="MRLFAAGLRKLVRRPATWVTFGLLVGLLTLIYLAVGVTAPQQTG
                     NGARQARLLLTFPGAYTLLLSFILGLGGLFAMIYGAAIAGSEWTWGTLKAAVARGESR
                     SRYQLLSFASIGFMVGIGLVVAFGLGIVVAIVAGGLAQVSMTGVDDAATLRTFPELLL
                     RGWLALGEACALGFAIATLARSQLAGIGVGIAVYFGEQFARIFLPDIVKYLPFNAANA
                     VVATSGGSIGSGGGDAAALARLDPNTALLVVLAWLVGALVVSALFTERAEISG"
     gene            complement(10425..11414)
                     /locus_tag="VHM48_01450"
     CDS             complement(10425..11414)
                     /locus_tag="VHM48_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2754094.1"
                     /translation="MTDGLALATRGLQKRYGSRMALAGLDLSVPTGVVYGFLGPNGAG
                     KTTTMRLLTGLIHPDAGTIEVLGQPFGRSDRRHLFDVGALIESPSFYPYLSARENLRS
                     LAASGARTTRSRIEELLELVGLRDRARDKVQTYSLGMKQRLGIAAALLSDPRLLLLDE
                     PSNGLDPAGIVAMREMLKQLALNGKTIFVSSHILGEVEQLADVLGIIAAGRLVREGPI
                     AHLLAGEGLVRVRVAPSEVAAARRILDGMVPDTASTPDGDGGWLSLRITPDRAAELNR
                     SLAAAGIYASGLESGNDLESLFLELTAGDDAASHEGTFFGSAGGNVSAGGGVR"
     gene            11560..12798
                     /gene="trxB"
                     /locus_tag="VHM48_01455"
     CDS             11560..12798
                     /gene="trxB"
                     /locus_tag="VHM48_01455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2754095.1"
                     /translation="MADDPIPSTPNDNATPVTKPATPVDPFGGLSIDFGGGPSATPDG
                     IANGSTAAPVSFGAPVSFGAPLETPATPIARSGPGGPVGGPVGGPDDGADRDANRTKV
                     LIIGSGPAGLTAAIYAARANLEPIVLAGVVPGGQLMITSDVENYPGFPDGILGPELMG
                     KFREQAERFGSRVLDVDVERVDFSERPFRAWARGIEYRADAVIVATGASALWLGLESE
                     TRLRGRGVSACATCDGFFFRDKEIAVVGGGDTALEEATYLTRFATKVHLLHRREAFRA
                     SKIMIDRAIDHPKIEIHTNTAVDEVLGGTKVEGLRLRDTATDEVRDVPMEGLFVAIGY
                     RPNTEVFKEQLEVDEKGYLIVHDETGSKIDGVFIAGDVHDHRYRQAVTAAADGCKAAI
                     DAERWLEAEGISEAATATAW"
     gene            complement(12980..13195)
                     /locus_tag="VHM48_01460"
     CDS             complement(12980..13195)
                     /locus_tag="VHM48_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2754096.1"
                     /translation="MELLGRWVRESRTRAGMTQAQLARLAGMHQTTLSRLERGKLEGL
                     RLHRLAAVIAVLDAALADRLPGLIAGQ"
     gene            13628..14116
                     /locus_tag="VHM48_01465"
     CDS             13628..14116
                     /locus_tag="VHM48_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2754097.1"
                     /translation="MKHRSATLVHEGGMRFAAETGTGRKLLFGDEPDRNEQSPVEIVA
                     VALAACSAMDVVSILEKKRQVVDSYRIDVRAEQRADYPQVYTRIDVTHIVEGTVVLEA
                     AVRRAVELSATKYCPVNAMLSAGATEVHHHFRMRCTGLEPKEASGEVMVTGPERRPDI
                     IT"
BASE COUNT         2134 a         4939 c         5097 g         1990 t
ORIGIN      
        1 aatcggagca gccccggcgc ccgtctcgcg tccgtcctgg cccacagcga cagcgccgcc
       61 cggatcggcc gcgccgcgct cgcggggggc ttcgtggagc gcgacgtccg cggcctgctg
      121 gccggcctcg caggatcgat cccggacctg gatgcgctcc tgcgcgacgg cggcgacgac
      181 gacgtccggg ccgccctcga cgtggcgcgg cgccgggagt ttgccgcccc cggcgccgcc
      241 gtgctgcgga tcgatggctg gatcgtggcc ccgacggagg cccgcgcctg cgccctgatc
      301 gcgttggcct gacagcgcgc ccgggttcgt caggtccgag cgtgctccgg ggcgtagtcc
      361 tcgacctcga aggtctggcg catcggcacc tcgtggagga acagcacgag cacggccgcc
      421 acggcccccc cgatgatccc gatccagaac gtcgaggcga tcgcgatcga gaaggcctcg
      481 tggacgccct tgacgatcgc ggggatcagg ccctgcccct cgggcgggat gacggtcgcg
      541 agccgtccgg cgagatcgcc cgtcccgaac agatcgagcc tggtcgcaac tgggctatcg
      601 ggcgagaacc cggtcaggaa cgccggcgga acgccggcct tgacgatctg ggccggcagc
      661 tcggtcgtga tcttgtcagc gagaagggtc ccggccagcg tcagcccgac ggttccgccg
      721 atctgctgga agaacgtcag gctcgaggtc gcgaccccga tccgatccgg cgacacggcg
      781 ttctggacaa tcagcgtgaa caccgcgaac gacggcccga tcccgagccc ggcgagggcc
      841 atccacagcc acatgaccgg ccggtccgtg tcggcccgga ggttcgtgaa caagaggagg
      901 gccagcgcga ggagcgcgag ggacccggcc atcaggatct tgtagcgacc gacccgggcg
      961 acgatctggc cggatgcgat cgcgctgaag atcaggccgg caagaagcgg caggaggttg
     1021 tagccggact cggtcgcgct cgcgccgccg acggtctgga accagcgcgg caggaagatg
     1081 accgcggtga agaacccgaa cgcggcgagg aacatcgacg cgaccgagat cgagaaggtc
     1141 cggagccgga acaggtcgag cggcacgatc gggtccaccg cccgcgtttc gacccacaga
     1201 aagatcgcgc cgaagacgag gcccacgagc atcagcccgc cgaccgctgg gtcggtccac
     1261 tggccggtct gcttgttcga cagccccacc aggaacggcg cgatcgccgc cgtgaagacc
     1321 gccgctccga cgtagtcgat gtgctccgcc gcgtcgggcc gcttgatggc cggcagcagc
     1381 cgccacagga tgaacagcga gacgagcccg atcgggatgt tgatgtagaa gatccagttc
     1441 cagctgagcg tatcggtgat gaacccgccg agcccggggc cgaccagcga ggacaccccg
     1501 aagaccgccc cgaacaggcc gaggtacttg ccccgctcag ccggcgtgta gaggtccgcg
     1561 acgaccgcga gggcgatcgg gaacaccgcg ccgccgccga ggccctggag cccgcggaac
     1621 aggatgaaca tctccatgtt ctggctgaga ccggcgaggg ccgagctgat caagaacagg
     1681 ctgaccgcga agatgacgat cgggcggcgg ccgaacaggt cggacagctt gccgtagatc
     1741 gggccgctga tggtcgacgt caggaggtag atcgtgaccg accacgtgta gagcgtctcg
     1801 ccgtggagag ccgagatgat ccgcggcagg gcggtcccga cgacggtctg gtcgagggcg
     1861 aacaggaaca tcgcgaggag gatcgcgccg aggatctcga gcttctggcg cggcgtcagg
     1921 ttgatggctg cgtcggacgt gggatgcggc atcgaatcac tcctgggggt tgggcagcgg
     1981 acatcatgtc agactggcgg agtccgcaga actcatcgct gacgcgcggc ctcgcagacg
     2041 acccccgggc cgagttcgaa tagccgaata ccgcgggcaa ccgggcacgg ggactcgacg
     2101 ttccgagcca ggcaccgctc aaacgccgat cgccgacatg cggctattcg aaccagcagc
     2161 gcatctccgt tgggaggcgt ggcgcgcggc ggcgcgcacc gctcaaacgc cgatcgtcga
     2221 tatgcggcta ttcgaaccag cagcgcatct ccgttgggag gcgtgggcgc gcaccggcca
     2281 gcccgcgacg atcacgggct ggccgggagc gaccgggacg acgacgccgg ccgccccgtt
     2341 gacgttcgcg tggcgggcgt acggccggtc ctgggtgcgc cggtgttcgg gtcgatgatc
     2401 tccggctggt acgggatcaa ccggagctcg acggcggccg actacctcgc ggccgcccgc
     2461 cgacacccgg cattcgagct ccgcccggtc aggtcgtgac gcagggagat ggcggatggt
     2521 ttccgtcgtc gatccggacg ttgaccgtct cgaaggcgat caccgcgtcg ctgcgaaccg
     2581 tcagaagcgc gttccggacc tcatcgacag gcggtcccgc cgggtacgcc ccggcgatcc
     2641 ggatcagccg gccccgacct tcggccaaca cgccccggac agagctcaac tggttcagca
     2701 gaccggtgct cgagctgtcc tgcgcgaagg cgtcggcgtc ggccaggctt gaatcgcccg
     2761 ttccggtcac gccgtcctgg ttgcagacct gagcatcgat cgtctcgatc agcgtgccga
     2821 tggtcgagcg cgtcgagatc aggcccccca ggatcggtac cggcggatgg tcggcgggca
     2881 ggctggcgac gatccggtcg agccggccgc ccatcgcgag gacaacgtgg tcgaccgcgg
     2941 cgatccggtt cgagatcgac aggctctggt cgtcgaccga gctgaccggg atgcgctccg
     3001 gggcggcaaa cgtcccgccg gcgagcgctc cgatgaggat cgtcgagctg agcccgccgg
     3061 tgatcagggt gcggattcga atcatgagtt ggctcctttc gttcgggcca cgaggtggtg
     3121 atgaggcggg acggccgtcg gcaggccacc gaggtcctgg gccgtcgcct cggagattcg
     3181 atcggcggtc aggccagccg cggcggcggc tgcccggagc gcgtcgatcg acggggcgtc
     3241 gaacagccag aacacggtgg cgtcgtcggg cacggtcact gcgcccaggt agcggacgtc
     3301 gccatgggcc gacgcctcgg cggcccgggc gaggcgctcc gggtcggccc ggcccgccga
     3361 cggcggccag tagccttcga tgaggtaggt cacgcgcagc tccgatccgg cgatgaaggt
     3421 ggcacccgac ggcaggctgg ccgccgggcg ccctggaccg atgtccctac ttggcctgga
     3481 gcttgaacgt ggccgaggcc gcgctcggga ggtagcgggc ggcgtcgctg ccggccggtg
     3541 tccacgtcgc cgtgagcggg taggtcccgt ttttctgggt gagcttgagt tggacgctcg
     3601 cgacgccgga cgcggaggtg accgcggagg tcgtctgcgc tccgagcttg aacgcgatcg
     3661 ttcggccggc cagcggcgtc ccggtcgcat caaccagggt cgccgacaac gtgaccgtct
     3721 tgttcgcgcc gccggtgagc gtcccggtgt acgccgtcgt cgtcgccttg cgggcgatcg
     3781 acacgttgcc gccgccggag gcagcgctgt agaacgtgtc gccggcgaag ctcgccgtga
     3841 tcccgtacga accggccgcg agacccgggg tccaggccgt cgaggcgagg ccggtcgagc
     3901 tcgtgaccgc cgatccggac gcgccgggta cggtctcgac cgcgaaggac acggtcctgc
     3961 cgttcacggc ctggccgaac tcgtcgacga gcgaggcccg caggcgggcg ccggtatcga
     4021 acgttgccgc cgtgtcgccg accgcaccga cgctgacgtc gcgggcccgg atcgtgacca
     4081 accgcgtgtc gaactggcac gcgccgtggc ggtcgcagac ggtgagcatc gccgtgtacg
     4141 gctcattgac gggtgagggc gcggcgtagg catgggtcac gctcgggccg gagcccgtcg
     4201 cgtccggtgt cccgtcgccg aaccgccacc agtaggtcag ggtggactgg tcgtccgtgc
     4261 cggggtcgac tgcctgaccg gtgaacgcga ccgaccggcc ccacgccgcg atcgtgtcga
     4321 ggccggcatc gacgagcggc gcctggttga agatctcgac gctgaaggtc tgggttgcgg
     4381 tcaggccggt gacgtcggtg gcggtcagca gcccggagta cgtgccgctg ccctggaagg
     4441 tgtgcttggg cttcgctccg aacgcgacgc caccgtccga gaagtcccag cggagcgtcg
     4501 ggaagccgca cgggctgctg ctggcggagc cgtcgaacgt gatcggcgtg ccctgatcgc
     4561 cgctgtactc gtggctggcg ccgccgccgg agtcgggcgt cggcgggatg ttgttcttgg
     4621 cgagcgcacc gagggtcgcc gagccggcgt tgcccgtcag cgcaagcggg acagcatgag
     4681 tcggagcagt gatggatgtg atgtcggacc cgacgacctc gatcggatcg agcagcggga
     4741 acttgatggc tgccgcggca tggagcgtcc cgcgaccggc gacgttcgtc gtcggagcgt
     4801 acggatcagc ctgcaggctg tacacgacgt cagcgccgga gggctgggtg cacgacaggg
     4861 cgatcgggtc cgcgagcgtc gacggtgtcg ctccgacgaa cgccagggag cggttgagcg
     4921 aaccgctgcc gccggtgaat gtgacggctc gcgtcgcgat gatgccatcg ctgctgacgg
     4981 tgaactcgag ccgaagctgg atcgagagaa cgaggttcag cgtgccggtc aggacggtcc
     5041 cggcgacagc gcgagaggcg agttggatgt cctgggtgtc cgacacgcac aacctggcgc
     5101 catcacccgg gagcggcatg gcgcatgagg ccgtccacgt cgcgttcttg tcgatgtcgt
     5161 ccgacgcgcc ggacgggccg aagttcgtgc cgcctgtcgg gtcgaccagc agcccggccg
     5221 agccctcgat cttgccggtc gcgacgatcg ccccgccggt tggggtcagg acgtcggaca
     5281 ggccgagcgt ctggccctgg cgcaggagcg cgtcgtcgta gttgagcgcg gtcgacgtcg
     5341 cagcctgcca tgcgacgtgg ttgacccggc ccgtggcggt ggcgccgtag gcccacctgc
     5401 cgccgccgag aagggtctcg aacacaccgg gcacacagtc gtcgcagacg ccactgacat
     5461 cgaagtcgta gccgaggtcg gatgtcgttt cgccctggta ggcgagcccg atcgtcctcg
     5521 tctgtggcgc tgccgtcgcc gagccgacgc tacccagaac gagcagcatg ccgccggcga
     5581 ggaccgccag gcgctgtcga agtctgttcc tgatcatgtg cttgacctcc accccggggt
     5641 tcggtctccg ccgcgctcat cgcggcgcga gtcgcccacg aaaggcacag tcgcctcggg
     5701 ccgcagcccg gacagccggg aaaaccctat ggatcgccct cacctgccct acgctgtggg
     5761 cgatggtcga cgtcctcggt cggatcacgg agcaggccgc cctcgaccgc ttcgtgaccg
     5821 aggcgcgaac gggtgcgcgc ggcctcgcga tcgagggcga ggccggaatc ggcaagtcca
     5881 cactgtgggc gagcgccgtt gcggctgccc ggtccggtcc gggtcaggcg tccgctggcc
     5941 tcgacccggc cgcgccaccg gccgtcgcca tcgtcctcga ggcgcgcccc gcatcgcccg
     6001 aggtccggct gtcgttctcg ggcctcgtgg acctgttcga gcccgtcgcc gacaccgtcc
     6061 tcggcgcgct ccccggcccg cagcgccagg cgctcgaggt ggcgctctat cgatcgccgg
     6121 cgggcgacag cagcgagtcg ggtctcgtcg gggtcgcggt cctgacggcg ctccgggcgc
     6181 tggcccgtca gcgacccgtg gtcgtggcga tcgacgacct gcaatggctc gatgcggcga
     6241 cgtcgatcgc gctccagcac gccgtccgtc gtcttcgctc cgaaccgatc ggcctgctga
     6301 cggcgacccg gaccggaccg gatgtcgagc cggccgtcga tctcgtgggt ctcctcgccg
     6361 aggacgccgc cgaacggctc cacctgggac cgttgccgat gggcgcgatc catcacctga
     6421 tccggacccg gctcgacgtc tccctgccgc gcccgacgct cgtccgtctc cacgccgatg
     6481 ccggcggcaa cccgctgtac gcgctggagc tcgcccgcca tgccgcgagc gcccccgacc
     6541 atttcgccag cgacgacaag gcgcgcgtcc cgagcccgct cgccgatctg ctccgccagc
     6601 ggctggcggg tctcgccgag ccgacccggg cgattctcct cgtcgtggcc ctggccggtg
     6661 gcgcgacgct cgacgagatc gcgcgcgcgg ccgggatccc gatcggcgac gcccgggcac
     6721 gcctcgagcc tgctcgccgg gacggcctcc tcgaacgatc cgggaacgag ctccggatca
     6781 gtcatccgct gattgccgcc gcgatcgccg actcggccga gccggacgcg cggcgctcgg
     6841 tccatcgacg cctcgccgag ctggccgacg agccggagac gcgggctcga caccttgcgg
     6901 ccggggcgtc ccagccggat cctgccgtgg cggcggcgct gtccgaggcg gccgagcagg
     6961 cgctggcccg cggcgcgatc gacgcatcgg tcgagctcct cgacgaggcg ctccgccact
     7021 ggccggcgga cgacgggccg ggccggcggc cgctccgcgt gaagctcgcc gagcggacgt
     7081 tcctggccgg cgacacggcc cgtgctcatc gcgacctcga ggcgctcctg ccggagctcg
     7141 tcgagcccga tctccgggtt cacgccgcga tcctcctcgg gacgatcgtg ttctacgacg
     7201 gggacagccg ggttgcctcg cagatcctcc tcgacgagct cgtgcgaacc gacgatcggg
     7261 tcggccgggg ccggatccat gcccgcctgt catggacgct cgagcacgac ctggccgccg
     7321 ccgcggagca cagccggctc gccgtcgaga cgctcgatcc cgcggaggcg cccgatatct
     7381 actcgtttgc gctgatgaac ctcgtggtgg tcgagttgga gctgggtcgc ggcgccgatc
     7441 acgaggcgat ggaacgcggt catcagatgc aggaacgggc gcagtcgtgg gaccacagct
     7501 cgctgcccgc gaactgggcg aagtggttcg acgacttcga gcggtcgcgg gtcctgaacc
     7561 atctctacct ggatcgcggt cgtcagaccg gcgacgagag ctccgttgcc cagctgctct
     7621 cctatctcgt cgagctcgag gcgtggaccg ggcacatgga cctcgccgag gtcgtcgcca
     7681 acgacgcggt cgccgcggcc gagcagacgg gccagccctc gtacctgtcg gccgccctcg
     7741 cccggcgagc tttcgtcagc gcgtcgctcg gcaggctcgc ggacgcgcgg gtggaagcga
     7801 cccgggcgac ccagctgggc gtcgagcttt caagtccatt gctcgaggcg ctcgggctcg
     7861 gcgtcctcgg cttcatcgag ctgtccgcag gcgctccgga ggtcgcggcc cgccacaccg
     7921 atcgggcgct cctcaagctc gacgagaccg gcatgttcga ccacccgatg ttccgcttcc
     7981 acgccgatca gatcgaggcc cacgtccgga tcggtgagct cgatgtcgcc gagcgactcc
     8041 tcgatcgcca gcgcgatcgg cagcgtcgag cgccgcgtcc gtggctcgcc ctgacggcgg
     8101 cccggaccgg ggcgctgctg gcggccgcgc gcggcgatgt gcccggagcc gtggccctcg
     8161 tcgacgaggc gttggccatc gcgccgaccc tcgcctggcc gatggagatc ggacggacgt
     8221 ggctcgtggc cgcggagatc cggcgccggg ccggacagcg caaggcagcg ggcgccgcgg
     8281 tcgccgaagc gatcggtcgg ttcgagtcga tcggcgcggc ggcgtggctg gcccgggcgc
     8341 gacgtgacgc cgccgccctc ggcatcgacc cgagcgagca tccggacagc ctcacaccga
     8401 gcgaggatcg agtcgcccgg ctcgcggccg gcgggatgac gaaccgcgag atcgccgccc
     8461 gcctcgccat cagcccgaag accgtcgagg cgtcgctcgc gcggacctac cacaagctcg
     8521 ggatccggcg gcgggccgag ctcgcgacgc ggctggcacg cgagacctga gcgccgacgg
     8581 ccacatcggc gcccgcaacc atcatcgcag ccgcgcgcgg cggcccggcg cctggcccgt
     8641 ggtccgtcgg tcgggcccgt cggacgcgat cggacagagg cgagtagcat tctgcaacca
     8701 actcgaccgc catcgtggag acccgcgtga agctcggact gcagattccc tcgttcacct
     8761 ggcccggcgg cacccccgcg atcggcccga ccctcgctcg cacggcccgt ctggccgacg
     8821 acatcggcct cgattcgatc tgggtcatgg accacttctt ccagatccgc ggtgtcgggc
     8881 ccgtcgagca ggcgatgctc gaaggctgga cggctctcgg cttcctcgcc gccaatacct
     8941 cgcgcgccag gctcgggctg atggtcggcg gcgtccacta ccggctgccc ggcttgtggg
     9001 tcaaggcggc gacgacgctc gacgtcctgt ccggcggccg ggcctggctc gggatcgggg
     9061 cggcgtggaa cgaggacgaa tcgcgcagcc tcggcttccc gttcccgccg ctcggcgagc
     9121 gcttcgagat gctcgaggag acgctccgga tcgcccacga gatgtggcag ggcgagcgcg
     9181 gctcggaggc ggccttcgac ggccggcagt tccacgcgac gcggctgatg aactcgccgc
     9241 agtcgatctc ccggccgcgc gtcccgatca tgatcggcgg cggcggcgag aagaagaccc
     9301 tccgcttcgt cgcccagtac gccgacgcga cgaacgtgtt tggcggcccc gaggcgatcg
     9361 cccacaagta cgagatcctg cgcgagcact gcgcggcggt cgggcgcgac tacgacgaga
     9421 tcgagcggtc gaacctccag acgatcaact tcggccggga gacgccgagc caggtcgtcg
     9481 agcgcttcgg cgagctggcc gacgccggcg cccagcacgt gatcttcagc gtggccgacg
     9541 tccacgatcc ggtccacctc gagacgatcg gccgggagat cctgccgcag ctccgcgacc
     9601 tgtaggcggt ccctagccgg agatctccgc tcgctcggtg aagagcgcac tgacgacgag
     9661 ggctccgacg agccacgcaa ggacgacgag cagggccgtg ttcgggtcga ggcgggccag
     9721 ggcggccgcg tcgccaccgc cactgccgat cgaaccgccg gacgtcgcga ccaccgcgtt
     9781 cgccgcattg aacggcaggt acttgacgat gtccggcagg aagatccgcg cgaactgctc
     9841 gccgaagtag accgcgatcc cgacaccgat gccggccagc tggctccgcg ccagcgtcgc
     9901 gatcgcgaag ccgagggcgc acgcttcgcc gagggccaac cagccacgga ggagcagctc
     9961 cgggaaggtc ctgagcgttg ccgcgtcgtc cacgccggtc atggacacct gggcgagccc
    10021 gcccgcgacg atcgcgacga cgatcccgag cccgaacgcg acgaccagcc cgatcccgac
    10081 catgaacccg atcgaggcga acgagaggag ctggtagcgg ctccggctct cgcctctcgc
    10141 gacggccgcc ttcaacgtcc cccaggtcca ctcggagccc gcgatcgcgg cgccgtagat
    10201 catcgcgaac agcccgccga gcccgagaat gaacgacagg aggagcgtgt aggcgccggg
    10261 gaacgtgagg aggaggcgcg cctggcgtgc cccgttgccg gtctgctgcg gagcggtcac
    10321 gccgacggcg aggtagatca gggtgagcag gcccacgagc aacccgaacg tgacccaggt
    10381 tgcgggtcgt cggacgagct tccggaggcc ggccgcgaac agcctcatcg gacgcctcca
    10441 ccggcgctga cgttgccgcc tgcactgccg aagaaggtgc cctcgtggct cgcggcgtcg
    10501 tcgcctgcgg tcagctcgag gaacagcgat tcgaggtcgt tgccggactc gagcccggac
    10561 gcatagatgc ccgccgcggc gagggaacgg ttgagctcgg ccgctcgatc gggggtgatc
    10621 cggagcgaca gccagccgcc gtccccgtcg ggcgtcgaag ccgtatcggg aaccatgccg
    10681 tcgaggatcc gccgggcggc cgcgacctcc gacggcgcta cccggacccg gacgaggccc
    10741 tcaccggcga ggaggtgcgc gatcgggccc tcgcggacga ggcggccggc ggcgatgatc
    10801 ccgaggacgt cggcgagctg ctcgacctcg ccgaggatgt ggctcgagac gaagatcgtc
    10861 ttgccgttca aggccagctg cttgagcatc tcgcgcatcg ccacgatccc cgccggatcg
    10921 agcccgttcg acggctcgtc gaggagcagc aaccgcgggt cgctcaggag cgcggccgcg
    10981 atcccgagcc gctgcttcat cccgagcgaa taggtctgga ccttgtcgcg ggcgcggtcc
    11041 cgcaggccga ccagctcgag cagctcctcg atccgtgaac gggtggtccg ggcgccgctt
    11101 gccgccagcg acctgagatt ctcgcgggcc gagaggtacg ggtagaagga gggcgactcg
    11161 atcagggcgc cgacatcgaa gaggtgacgc cggtcgcttc gtccgaatgg ctggccgagg
    11221 acctcgatcg tccccgcatc gggatggatc aacccggtca ggaggcgcat cgtggtcgtc
    11281 ttgcccgcgc cgttcgggcc gaggaacccg tagacgacgc cggtcggcac cgacaggtcg
    11341 agcccggcga gggccatccg cgagccatac cgcttctgca agccgcgggt cgcaagggcg
    11401 aggccgtcgg tcaagggaga tctccggtgg ggtgggcggc atgagcggtg tcgagcggcg
    11461 cccaggttcg gatcgtgcgg tggccggcct cgacctgtca aaccaggcgg ctgggcgata
    11521 ccccctgggg gtatggcttc cgtgctagga tgcgaggcga tggccgacga tccgatccct
    11581 tccacaccca acgacaacgc gaccccggtg acgaaacccg cgactccggt cgatcccttc
    11641 gggggccttt cgatcgactt cgggggcggc ccttcggcca cgccggacgg catcgccaat
    11701 ggcagcacgg cagcccccgt ctcgttcggc gcccccgtct cgttcggcgc cccgctcgag
    11761 acgcccgcga cgccgatcgc gcgatcgggg ccgggtggcc cggtcggtgg cccggttggt
    11821 ggccccgacg acggcgccga ccgtgacgcg aaccggacga aggtcctgat catcggttcc
    11881 ggtccggccg gtctgactgc ggcgatctat gccgcgcggg ccaatctgga accgatcgtc
    11941 ctcgccggcg tcgtgcccgg cggccagctg atgatcacga gcgacgtcga gaactatccg
    12001 gggtttccgg acgggatcct ggggccggag ctgatgggca agttccgtga gcaggccgag
    12061 cggttcggtt cgcgggtcct cgacgtcgac gtcgagcggg tggatttctc ggagcggccg
    12121 ttccgggcct gggcgcgcgg catcgaatac cgagccgacg cggtcatcgt cgcgaccgga
    12181 gcgtcggcgc tgtggctcgg cctcgagagc gagacccggc tccgcggacg cggcgtctcg
    12241 gcgtgtgcga cctgcgacgg cttcttcttc cgggacaagg agatcgcggt cgtcggcggc
    12301 ggcgacacgg ccctcgagga ggccacctac ctgacccgct tcgcgacgaa ggtccatctc
    12361 ctccaccggc gcgaggcgtt ccgcgcctcg aagatcatga tcgaccgggc gatcgaccat
    12421 ccgaagatcg agatccacac gaacaccgcg gtcgacgagg tcctcggcgg gaccaaggtc
    12481 gaggggctcc gcctccgtga caccgcgacc gacgaggtcc gtgacgtccc gatggagggc
    12541 ctgttcgtcg cgatcggcta ccgcccgaac accgaggtgt tcaaggagca gctcgaggtc
    12601 gacgagaagg gctacctcat cgtccatgac gagaccggct cgaagatcga cggtgtcttc
    12661 atcgccggcg acgtccacga ccatcgctac cgccaggctg tcacggcagc agccgatggc
    12721 tgcaaggccg ccatcgacgc cgagcgctgg ctcgaggccg aggggatcag cgaggccgcc
    12781 accgccaccg cctggtaggc cgcgtaggcc cgggcgccgc gggcgccgcg ggcgccgcgg
    12841 agcgccgccc ggcgcagccc ccggtggacg ctcgtcgccc tcgatcgccg cgccagattg
    12901 cgtccagctg agcgctgcga atagccgaat atctcgcggg cgtgcccgcg gccagctcga
    12961 gacctcgggt ccgtcgcggt cattgcccgg cgatgaggcc gggcaaccga tcagcgaggg
    13021 cggcgtccag gaccgcgatg acggcggcca ggcggtggag ccgcagcccc tcgagcttgc
    13081 ctcgttccag tcgcgacaac gtcgtttggt gcatgccggc gagccgagcc aactgcgcct
    13141 gggtcatgcc ggcacgcgtc cgggattcgc ggacccagcg gccgagcagc tccatgcccg
    13201 tcgtgacgat ccattcagga ctcgtcgcac gacgacacgg gttcgcgaca tgcggcctcc
    13261 gtgggacgcg cgtcgggcga tgccggttcg gatgcatgga cgacatcgaa cacgatcgtc
    13321 ctcatccgcg aatgcactga gcatgcactg ggacggaccg ccgctggccg atattcggct
    13381 attcgcaacg gcctcgcggt gtgtgttcgg cgctcgagcg cagcgccgag ccctccggaa
    13441 cgatccggac gaaccgtgcg ggcatctgaa cgcggaactg caatatctcg ttccgacggc
    13501 cgggtcttcg cggcctccaa cggcatccgc gccagggccg cggctccgtt cggtcccggg
    13561 cccgcccttc gccgagccgg tcgcgccggc agcgtgatcg cgggcgccga ccggctacga
    13621 tgaccgggtg aagcatcgat ccgccaccct ggtccacgaa ggcggcatgc ggttcgccgc
    13681 cgagaccggg accggccgga agctgctgtt cggggacgag ccggaccgaa acgagcagtc
    13741 gcccgtcgag atcgtggccg tggccctggc ggcctgctcg gcgatggatg tcgtctcgat
    13801 cctcgagaag aaacggcagg tcgtcgattc gtaccggatc gacgtccggg ccgagcagcg
    13861 cgccgactac ccgcaggtct acaccaggat cgatgtcacc cacatcgtcg agggcacggt
    13921 cgttctcgaa gcagcagtcc gccgggccgt cgagctgtcg gccacgaagt actgccccgt
    13981 caacgcgatg ctgtcggccg gggcgaccga ggtccatcac cacttccgga tgcgctgcac
    14041 cggcctggag ccgaaggaag cgtcgggcga ggtcatggtg accggcccgg agcgccgtcc
    14101 cgacatcatc acctgagcga cgccgcgagc ggcgcacgag cggttgcatg actatgcgtc
//
LOCUS       DATAIW010000040         7486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_31247, whole genome shotgun sequence.
ACCESSION   DATAIW010000040 DATAIW010000000
VERSION     DATAIW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31247"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VHM48_01470"
     CDS             complement(<1..1032)
                     /locus_tag="VHM48_01470"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX2754098.1"
                     /translation="MTADPILLLAWLVLAHLVADFVLQTGRVARLKSRSGTDAAAGLL
                     VHGLIVAGCLVPVGLAFADRGWAFVALVGVSHVVIDRTKVVLTRRAAADALRDAHERH
                     EGPQPAADHLGRAWTPRPAALFLADQAAHLAMSGWGWAALLATTPLTTGWTSAIDGWL
                     DGWDRATVHAVVGGFVVLVSLAIVNVRAASLFVGILVRPVEAGVDGEHRWGGRVAPAT
                     EPTPRDPSANVPTPRDPSANVPASTTRHWSIRLGPLDARVTAEPDPAPAPTSAAAPVR
                     SNPAPAPLGTSARVGSTIGILERILIVVFVLTGTDVAIGFVVAAKTLARFKLLDDRDF
                     AEYYLLGTLA"
     gene            complement(1029..1658)
                     /locus_tag="VHM48_01475"
     CDS             complement(1029..1658)
                     /locus_tag="VHM48_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754099.1"
                     /translation="MPAAIILFGDVIRSRRDAVAATAWLRVLCADLTEAYPAGALLAP
                     FEFTQGDELQGLLAPTADPMTAILRAGLHPEGVKMRWVVVAGEVDPGHGPATQRSGPA
                     FIAARERLAMAAARRERLAVVSGDPETDDLLDGLAPLLGELLAELTKRQREIAWPIIV
                     DGLRRSEAAEQLHVSRATISVAADRAHLRGLGELSRVVRTLLQRATMGA"
     gene            complement(1726..3294)
                     /locus_tag="VHM48_01480"
     CDS             complement(1726..3294)
                     /locus_tag="VHM48_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2754100.1"
                     /translation="MAELSLAGDGSADPRPFPPGDYPVVVVGSGAGAIQVSYSLRRRG
                     VDHAVVSADPSPGGMFRRWPFFQRLLSWTKPFAPVERDSPAYERYDWNSLLGDAPGER
                     AIMPDLMDGSSYFPSRPEMERNIATFAERTGTQVRYDCRWTATRREDRPDGAQFVLET
                     TDGEYRARSLVFAVGVAEPYSPATPGIELAAHYADTRPAETYAGRRIFIMGKQNSGFE
                     LATGLLPWARRIILCSPSPAKLSVNTKSLVGVRARYVQPFEDHVLGGGVSVLDASIGG
                     IVRGAAGSDSPIVVTVRPSDGSDDMAIEVDEVISATGFVTPLQDLPALGVSTFGQARL
                     PAQTPYWESATVPGIYFAGTIGQGSAGLKKHGLPANSGAVHGARYNARVLAGHIARDH
                     GPADPAPERRLLPATGLVDVVLEELATTPELWHQRAYLAKVVSLDPAVGPRDEGVVPL
                     AAFVDAVGDDGDGDALAVTLEADGSGAIYPVLYLRRGGKVEERAVEPDALLRFDTPAA
                     RVQIAGILSEAGIG"
     gene            complement(3282..3704)
                     /locus_tag="VHM48_01485"
     CDS             complement(3282..3704)
                     /locus_tag="VHM48_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2754101.1"
                     /translation="MATRDEIVDQIAGFALFADLTTPQLERIAHMFEEEVFPDGARVL
                     RQGLSGSAFYVILDGEAEVVIDGTQRATLARGDFFGEVSILLGEAPGADIVASRPLRT
                     LVLAGPAVKSVLLDNPPLMYRMLQAQARRLRNANRWRS"
     gene            3811..4239
                     /locus_tag="VHM48_01490"
     CDS             3811..4239
                     /locus_tag="VHM48_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2754102.1"
                     /translation="MTLTQDRRTELLGGCPLFGGVSQEDLAAIGARALEVDFPAEHVI
                     ARQGEIGTGLFVVTAGAVRVVRDGEELARLRTGDFFGEMSVIDGLPRVAQVITTEPTR
                     CLALASWEFERLVLEHPTIGLAILRGLSARLRAKTELPQH"
     gene            4268..7009
                     /locus_tag="VHM48_01495"
     CDS             4268..7009
                     /locus_tag="VHM48_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2754103.1"
                     /translation="MTTLPTGTVAFVFTDIEGSTHLAQTLTDERWEAILARHRELIRA
                     AVATHGGVEVSTEGDGFFLAFARTSDAVAAMVDAERALSAEPWPADALVRVRVGIHTG
                     DGRLDGEGSYVGADVHRAARVAAAGHGGQVLLSQTTSTLVADELPPGVGLRGLGEHRL
                     KDLRPERICQLVIEGERSEFPPIRSLDRRPNNLPTQLTSFVGRDAELREAGELLRTTR
                     LLTLTGPGGTGKTRLSLQLAADVSDRFADGLWFVALEPVRDPGLIAATILTTLGLVEA
                     GGRPARDILVEWLAPREVLLVLDNFEQVIDGAPVVAEILRGAARVSVIVTSRAPLRVS
                     GEQEYPVPGLPAPRDVLALSALEKMNLPAGDRLLDAAAATQFEAVRLFIARAVAVRPD
                     FRVTNENAPAVAGIAARLQGMPLAIELAAARVKLLPPEMILNRLEHQLGVLSVGSRDL
                     PERQQTLRGAIAWSHALLGPGERRLLARLSVFVGGCELDSAEAVCGPPDELDGVDVID
                     CLTFLVDQSLVRAEDVDGGLRFRMLDTIREFAAEQLAANGERDAILTRHGGAVVNLVE
                     TLTARLSGDDQRRWLGRLERDHDNIRAVLDRAVAAGDASAAIRIGFAMWRYWQKRGHL
                     AEARRRLQAMADAPWSRDDPRLRARLMEALGGVCWWQADITAMGVAYAEALELWQAIG
                     DRREIANALYNDSFRYALSDTPGERDPERLGYKQMHRARDLATEAGDERGRANALWGI
                     GNWLYFHGGEDGGASQFDEALVVFRRLGDRTMEAWSHHMLGTSQIRLGTLEEARRNVS
                     AAMRLFHGFGDVAGLALTLDDFASIAIAEGNLPRAAKLWGTARALSAAGGVGIADFVE
                     SQFEFYGRPSTRESVDPTELDRLAEEGRAMTLDESVAYALELDIDALAPHDHAGAIR"
     gene            7006..7185
                     /locus_tag="VHM48_01500"
     CDS             7006..7185
                     /locus_tag="VHM48_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754104.1"
                     /translation="MIEPQSVLPILMLDDDEPPAPGPATASRLGPDDDPGVLVLTCAN
                     CGAAMDERKCKLICR"
BASE COUNT         1052 a         2713 c         2753 g          968 t
ORIGIN      
        1 ggccagggtg ccgaggaggt agtactccgc gaagtcgcgg tcgtcgagga gcttgaaccg
       61 ggcgagggtc ttggcggcga cgacgaatcc gatcgccacg tccgtcccgg tgaggacgaa
      121 gacgacgatc aggattcgct cgaggatccc gatggtcgac ccgacgcggg cgctcgtccc
      181 gaggggcgcc ggcgccgggt tcgaccgcac gggagccgcg gcggacgtcg gcgccggagc
      241 gggatcgggc tcggcggtga cccgcgcgtc cagcggcccg agccggatcg accagtgacg
      301 ggtcgtggac gccggcacgt tcgcggacgg atcgcggggc gtcggcacgt tcgcggacgg
      361 atcgcggggc gtcggctcgg tcgccggggc cacccgtccg ccccagcgat gctcgccgtc
      421 gacgccggcc tcgaccggcc ggacgaggat cccgacgaac agcgacgcgg cgcggacgtt
      481 gacgatcgcg agcgacacga ggacgacgaa ccctccgacg acggcatgga ccgtggcacg
      541 gtcccagcca tccagccagc cgtcgatcgc cgacgtccat ccggtcgtca ggggcgtggt
      601 cgcgagcagg gccgcccagc cccagcccga catcgcgagg tgggcggcct ggtcggcgag
      661 gaacagggcg gccggtcgcg gtgtccaggc ccggcccagg tggtcggcgg ccggttgggg
      721 gccctcgtgc cgctcgtgcg cgtcgcgcag ggcgtcggcc gccgcccgcc gggtcaggac
      781 gaccttcgtc cggtcgatga cgacgtggct cacgccgacc agcgcgacga aggcccagcc
      841 acggtcggca aacgcgaggc cgaccggcac caggcagccc gcgacgatca ggccgtggac
      901 gaggagcccg gcggccgcat ccgtcccgct tctcgacttc aggcgcgcga cccgcccggt
      961 ctgcagcacg aagtcggcga cgagatgggc gaggacgagc caggcgagga ggaggatcgg
     1021 gtcggcggtc atgcgcccat ggtcgcccgc tggagcaagg tccgcacgac gcgcgacagc
     1081 tcgccgagtc cgcgcaggtg ggcacggtcc gccgcgacgg agatcgtcgc ccggctcacg
     1141 tggagctgct cggccgcctc ggatcggcgc aggccgtcga cgatgatcgg ccaggcgatc
     1201 tcgcgctgcc gcttcgtcag ctcggcgagg agctcgccga ggagcggggc cagcccgtcg
     1261 aggaggtcgt cggtctcggg atctccgctg acgacggcga gtcgctcgcg ccgggcggcg
     1321 gccatcgcca gccgctcgcg ggcggcgatg aacgcaggcc cgctccgctg cgtggccgga
     1381 ccgtggcccg gatcgacctc accggcgacg acgacccagc gcatcttgac gccctcggga
     1441 tggaggccgg cccgcaggat cgcggtcatc gggtcggccg tgggcgcgag cagtccctgg
     1501 agctcgtcgc cctgggtgaa ctcgaacggg gccagcagcg cgccggccgg ataggcctcg
     1561 gtcagatcgg cgcacaggac gcgcagccac gccgtggccg cgaccgcgtc acggcgcgag
     1621 cggatgacat ccccgaacag gatgatggcg gcgggcacgg agggaggcta actgaccacg
     1681 aattagccgt caacgactaa ttccgctgcg attagtcgtc gaaggctagc cgatccccgc
     1741 ctcgctcagg attccggcga tctgcacccg ggccgccggc gtgtcgaacc ggagcagcgc
     1801 gtcgggctcg accgcccgct cctcgacctt gccgccgcga cgcaggtaca ggaccgggta
     1861 gatcgcgccg ctgccgtcag cctcgagggt gaccgccagg gcgtcgccgt cgccgtcgtc
     1921 gccgacggcg tcgacgaagg ccgcgagggg cacgaccccc tcgtcgcgcg ggccgaccgc
     1981 cgggtccagc gagacgacct tcgcgagata ggcgcgctga tgccacagct cgggcgtggt
     2041 ggcgagctct tcgaggacga cgtcaacgag cccggtcgca ggcagcagtc gacgctcggg
     2101 agccgggtcg gcgggtccgt ggtcgcgggc gatgtggccg gccaggaccc gggcgttgta
     2161 gcgcgccccg tggaccgctc ccgagttcgc cggcaggccg tgcttcttca acccggccga
     2221 accctgcccg atcgtcccgg cgaagtagat gccgggcacg gtcgcgctct cccagtacgg
     2281 ggtctgggcc ggcagccgcg cctgaccgaa cgtcgagacg cccagggccg gcaggtcctg
     2341 gagcggcgtg acgaagccgg tcgccgagat gacctcgtca acttcgatcg ccatgtcgtc
     2401 actgccgtcg gacggccgga cggtcacgac gatcggcgaa tccgaacccg cggcgccgcg
     2461 cacgatcccg ccgatcgacg cgtcgaggac cgagacccca ccgcccagga cgtgatcctc
     2521 gaacggctgg acgtaccgcg cccggacgcc gacgagcgac ttcgtgttga ccgacagctt
     2581 ggccggcgac ggcgagcaca ggatgatccg gcgggcccac ggcaacaggc cggtcgcgag
     2641 ctcgaagccc gagttctgct tgcccatgat gaagatccgc cggccggcgt acgtctcggc
     2701 cggtcgggtg tcggcatagt gggccgcgag ctcgatcccg ggcgtggccg gcgagtacgg
     2761 ctcggccacg ccgacggcga agacgaggga cctggcgcgg tactcgccat cggttgtctc
     2821 gaggacgaac tgtgccccat ccggacgatc ctcacggcgg gtcgcggtcc agcggcagtc
     2881 gtagcggacc tgcgtcccgg tccgctcggc aaaggtcgcg atgttccgct ccatctcggg
     2941 ccgcgacggg aagtaggacg acccgtccat gaggtccggc atgatcgcgc gctcgcccgg
     3001 ggcgtctccg agcagggagt tccagtcgta gcgctcgtac gccggggagt cgcgctcgac
     3061 cggcgcaaac ggcttcgtcc aggacagcag gcgctggaag aacggccagc gccggaacat
     3121 cccgccgggc gacgggtcgg ccgacacgac ggcgtggtcg acgccacgac ggcgcagcga
     3181 ataggagacc tggatcgcgc ccgctccgct gccgacgacg acgaccggat agtcgccggg
     3241 cgggaagggc cgtgggtcgg ccgagccgtc gccggccagg ctcagctccg ccaccggttc
     3301 gcgttccgca gacggcgcgc ctgcgcctgg agcatccggt acatgagcgg cgggttgtcg
     3361 agcagcacgc tcttcaccgc cgggcccgcc aggaccagcg tccgcagcgg tcgcgaggcg
     3421 acgatgtcgg cgccgggcgc ctcgccgagg aggatcgaga cctcgccgaa gaagtcgccg
     3481 cgggcgaggg tcgcccgctg ggtgccgtcg atcacgacct cggcctcgcc gtcgaggatg
     3541 acgtagaagg cggatccgga gagtccctgg cggaggacgc gggccccgtc ggggaagacc
     3601 tcctcctcga acatgtgggc gatccgctcg agctgcgggg tggtcaggtc ggcgaacagc
     3661 gcgaacccgg cgatctggtc gacgatctcg tcgcgggtgg ccatcgggcg tgcgtcctct
     3721 cttccgttcg gttgctccat tctgccaccc ggcgcggttg acgggggccc gatcgaacgc
     3781 gcagcatcag gccctgccag aggaggaccc gtgacgctca cccaggaccg ccgcaccgag
     3841 ctgctgggcg gctgcccgct gttcggcggc gtcagccagg aggacctcgc cgcgatcggc
     3901 gcgcgcgccc tcgaggtcga ctttccggcc gagcatgtca tcgcccggca gggcgagatc
     3961 ggaaccggcc tgttcgtcgt caccgccggg gccgtccgcg tcgtccgcga cggggaggag
     4021 ctcgcccgcc tccggaccgg cgacttcttc ggcgagatgt ccgtgatcga cgggctgccg
     4081 cgcgtcgccc aggtgatcac gaccgaaccg acccgctgcc tggctctcgc cagctgggag
     4141 ttcgagcgac tggtcctcga gcacccgacg atcgggctcg cgatcctccg cggcctctcc
     4201 gcccggctgc gggcgaagac cgagcttccg cagcactgac ggacctgcca cgcctcccga
     4261 ccgctcgatg accacgctgc cgaccggcac ggtcgccttc gtcttcaccg acatcgaggg
     4321 ctcgacccat ctcgcccaga cgttgaccga cgagcgctgg gaggcgatcc tcgcccggca
     4381 ccgcgagttg atccgggcag ccgtcgcgac ccacggcggc gtcgaggtct cgaccgaggg
     4441 cgacggcttc ttcctcgcct tcgcccggac ctccgatgcg gtcgcggcga tggtcgacgc
     4501 cgagcgggcg ctctccgcgg agccctggcc ggcggatgcg ctcgttcgcg tccgggtggg
     4561 catccatacg ggcgacggtc gcctcgacgg cgagggctcg tacgtcggag ccgacgtcca
     4621 ccgggcagcg cgggtcgccg ccgccggtca cgggggccag gtcctgctct cccagacgac
     4681 ctcgaccctc gtcgccgacg agctgccgcc cggcgtcggc ctccgcggtc tcggcgagca
     4741 tcgtctcaag gacctccggc cggagcggat ctgccagctc gtcatcgagg gcgagcggtc
     4801 cgagttccca ccgatccgct cgctcgatcg tcgtccgaac aacctgccga cccagctgac
     4861 gagcttcgtc ggccgcgacg cggagttgcg cgaggccggc gagttgctcc ggacaacccg
     4921 gctcctgacc cttaccggac ccggtggaac gggcaagacc cgcctgtccc tccagctcgc
     4981 cgccgacgtc agcgaccgct tcgccgacgg cctgtggttc gtggcgctcg agccggtccg
     5041 ggaccctggc ctgatcgcgg ccacgatcct cacgacgctc gggctggtcg aggcaggcgg
     5101 tcggccggcc cgcgacatcc tcgtcgagtg gctcgcgccg cgcgaggtcc tcctcgtcct
     5161 cgacaacttc gagcaggtca tcgacggcgc gcccgtcgtg gccgaaatcc tgcgtggggc
     5221 agcgcgggta tcagtcatcg tcacgagccg ggcgcccctc cgggtgagcg gcgagcagga
     5281 gtacccggtc ccgggcctgc cggcgccgcg cgacgtcctc gccctgtcgg ccctcgagaa
     5341 gatgaacctg cccgcgggcg accggctcct cgacgcggcg gccgcgaccc agttcgaggc
     5401 ggttcgcctg ttcatcgccc gcgccgtggc ggtccgcccc gacttccggg tcaccaacga
     5461 gaacgcgccg gcggtcgccg ggatcgcggc ccgcctgcag gggatgccgc tggcgatcga
     5521 gctggccgcc gcccgggtca agctcctccc gcccgagatg atcctgaatc ggctggagca
     5581 ccagctcggg gtgctgtcgg tcggatcgcg cgacctgccc gagcggcagc agacgctgcg
     5641 cggggcgatc gcctggagcc acgccctgct cggccccggc gagcgccggc tgctcgcccg
     5701 gctgtcggta ttcgtcggcg ggtgcgagct cgacagcgcc gaggcggtct gcggcccgcc
     5761 cgacgagctc gacggcgtcg acgtgatcga ctgcctgacg ttcctcgtcg accagagcct
     5821 cgtccgggcc gaggacgtcg acggcggcct gcgcttccgg atgctcgaca cgatccgcga
     5881 gttcgccgcc gagcagctgg ctgcgaacgg ggaacgtgac gcgatcctga cccgccacgg
     5941 cggcgcggtc gtcaacctcg tcgagaccct gaccgcgcgc ctgagtggcg acgaccagcg
     6001 ccgctggctc gggcggctcg agcgcgacca cgacaacatc cgggcggtcc tcgaccgggc
     6061 ggtcgcggcc ggcgatgcct cggccgcgat ccggatcggc ttcgcgatgt ggcgctactg
     6121 gcagaagcgg ggccacctgg ccgaggcacg gcgccggctc caggccatgg ccgacgcacc
     6181 gtggtcgcgc gacgacccgc ggctccgggc acgcctgatg gaggcgctcg gcggggtgtg
     6241 ctggtggcag gccgacatca cggcgatggg cgtcgcctac gccgaggcgc tcgagctgtg
     6301 gcaggcgatc ggcgacaggc gcgagatcgc gaacgcgctc tacaacgact cgttccggta
     6361 cgccctgagc gacacgcccg gcgagcgcga cccggagcgg ctgggctaca agcagatgca
     6421 ccgggcgcgc gacctggcca ccgaggccgg cgacgagcgg ggccgcgcca acgccctctg
     6481 ggggatcgga aactggctct acttccatgg cggagaggac ggcggcgcga gccagttcga
     6541 cgaggcgctc gtcgtgttcc ggcggctcgg cgaccggacg atggaagcct ggtcccatca
     6601 catgctcggc acctcgcaga tccggctggg gacgctcgag gaggcccgtc ggaacgtctc
     6661 ggcggcgatg cgcctgttcc acggcttcgg cgatgtcgcc ggactcgcgc tgaccctcga
     6721 tgacttcgcc tcgatcgcga tcgcggaagg caacctgccg cgggcggcga agctgtgggg
     6781 caccgcccgc gccctgtccg ccgccggcgg cgtcggcatc gccgacttcg tcgagagcca
     6841 gttcgagttc tacggccgac cgagcactcg cgagtccgtc gacccgaccg agctcgaccg
     6901 gctcgccgag gagggccggg cgatgaccct cgacgagagc gtcgcgtacg ccctggagtt
     6961 ggacatcgac gcgctcgcgc cgcacgacca cgcgggtgcg atccggtgat cgagccgcag
     7021 tccgtgctgc cgatcctgat gctcgatgac gacgaaccgc cggctccggg accggccacg
     7081 gcgtccaggc tcggccccga tgacgatccc ggcgtcctcg tcctgacctg cgccaactgc
     7141 ggagcggcga tggacgagcg gaagtgcaag ctgatctgcc gctgagggcc gccgcgcggg
     7201 cagggccctg gtcgcgcaag cgaggctccg gtccacgccg cggaccaagc gacggccgtc
     7261 ccctggcacg gctcgccgag acgcggattt cgacccttcg gctgccgcct ggtcctcacc
     7321 gtggaccgcc cgacggcagg accgacccga ggctctcggg ccgggggcgt cgacggtccc
     7381 ttggtccgcg gaccggacca gcaaccgtcg aggggggacg gcgcaggcgc ccggcgggtg
     7441 cggcacggaa cccacgatac ccgtccggcg ggcctaccga gcacga
//
LOCUS       DATAIW010000041         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1295, whole genome shotgun sequence.
ACCESSION   DATAIW010000041 DATAIW010000000
VERSION     DATAIW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHM48_01505"
     CDS             <1..203
                     /locus_tag="VHM48_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754105.1"
                     /translation="PFMVPFATDAKHTAALDVPTYGFSPLRLDPEERFMERFHGVDER
                     VGIDALRWGLPVLYDVVRRYCG"
     gene            complement(286..864)
                     /locus_tag="VHM48_01510"
     CDS             complement(286..864)
                     /locus_tag="VHM48_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2754106.1"
                     /translation="MTVESRPGRRVGPPPPARAIARRFEADLSATVLAAGTRISNVRQ
                     ISERVAMGRALWRELVIGFASQLGEMRLGFTQLAALYVLADSGTMTIGDLAEAINRSP
                     SATSRLVDGLVRRRLVERRVEEEDRRQRTLQLTPRGAALLRVVDRARADQFLSAVRPM
                     PTAERALIAMGVAALATHAISRRGRLIKNRTE"
     gene            996..1964
                     /gene="fabD"
                     /locus_tag="VHM48_01515"
     CDS             996..1964
                     /gene="fabD"
                     /locus_tag="VHM48_01515"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2754107.1"
                     /translation="MTSSAFVFPGQGSQSVGMGQALAAASPAAASVLAAADAALGESI
                     SRLAFDGPADLLDRTENAQPALLAVSIAYLAALRERWAALGIEAPVPAFAAGHSMGQY
                     SALVAAEVISIEDGVRLVRERGRLMQASGAGRDGAMAAILGLDDAALPALVERASAAG
                     VFGVANRNAPGQVVVSGERAAIEVAASIAKELGARKAIVLPVSVAAHSPLMSEAAEGM
                     RRALADVSFHDPVVPLLANADARPITTADDARAELVEHLTAGVDWVAAVERMTAAGVT
                     TFIEVGPGRVLTGLIKRIAPDADVLALDDPGSPDRLTVPFAMATTA"
     gene            1995..3269
                     /locus_tag="VHM48_01520"
     CDS             1995..3269
                     /locus_tag="VHM48_01520"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX2754108.1"
                     /translation="MRKPDYDRRVVVTGLGVISPVGNDVQTAWGNLVRGQSGLTEITK
                     FDVSRYEAKAAGEVHDFNPTDWMDPKAVRRSESAMWFGVAAGKQALADSGFEITDANR
                     EEVGVVFGSGAGGQQLMIDAWINLHEKGPRSVPPTFIANALVDSASGMIAIETGAIGH
                     NVCIVSACATGTHNVSEGAEAIRRGDCIAVISGSTEAPLLETAHAGFSNMRGMGMPRP
                     GEGLESVSRPFDKTRDGFVLGEGAGGLFLEDLELAKARGARIYAEVVGYGSAADGWDM
                     IQPIAGGSGSARAMKMALARRGVPADEVDLINPHGTSTPLGDQREAEAIWTVFGDRAA
                     AARHSLAISGTKSMTGHMMGAAGAFEAFATVMSVAEQCIPPTINYRDFDPVCDLWVVT
                     EATPREIRYGLSNNIGLGGHNGAVIFKRYDGD"
     gene            3427..>4575
                     /locus_tag="VHM48_01525"
     CDS             3427..>4575
                     /locus_tag="VHM48_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX2754109.1"
                     /translation="MPELDPSRRAVVTGLGAVMPIGNDFPTYWSNLVAGVTGTRLIRS
                     FDASAFEVRIAAEVLDFDPTTVMDAKMARRMTRFIHLGMGAAKEAVANSGIDFAAMTE
                     DERDRVGVVMNTGGGGIEAIIDGTHVHDQKGPRFVSAFAVPALSGSMGAALLSMEWHL
                     TGPVMTQVAACATSVIAFHDALRLIRTGECDVVLTGGSEAPVVPMGVAALANMGALSK
                     RNDSPETASRPFDGTRDGFVLGEGAGLVVIESLAHALARGATPIAEVLGGALTADAFH
                     ISAPEPTGRADEVTDHRLRRADRDLPGMSPVGRLDRPCLGHVVEGCRRSVGDDVVDLV
                     GGDVAVAERVRHRPGLAAAGRLGRADMEGIGGQGPSENLGDRRCATGES"
BASE COUNT          654 a         1648 c         1596 g          677 t
ORIGIN      
        1 tcccgttcat ggtcccgttc gcgaccgacg ccaagcacac cgccgcgctc gacgtcccga
       61 cctacggctt ctccccgctc cgtctcgacc ccgaggaacg gttcatggag cgcttccacg
      121 gggtcgatga acgggtcggg atcgacgcgc tgcgctgggg attgcccgtc ctgtacgacg
      181 tggtccgccg ctactgcggc tgatccgacc ccggccgggc gctccgcgga acgcggttca
      241 gcggtgcgca tcgtcgccgc atccagcgcg acccgcaagg ccgccctact ccgtgcggtt
      301 cttgatcaag cgtccgcggc gcgagatcgc gtgggtcgcc agggcggcga cgcccatcgc
      361 gatcagcgcc cgctccgccg tcggcatcgg tcggacggcc gacaggaact gatccgcccg
      421 ggcccggtcg accacccgca gaagcgccgc ccctcgcggc gtcagctgga gcgtccgctg
      481 gcggcggtcc tcctcctcga cccggcgctc cacgaggcgt cgccggacca gcccgtccac
      541 cagccggctc gtcgccgacg gtgagcgatt gatggcctcg gcaaggtccc cgatcgtcat
      601 cgtccccgag tcggccagga catacagggc cgccagctgg gtgaacccga gccgcatctc
      661 cccgagctgg gaggcgaacc cgatgacgag ctcacgccac agggcgcgac ccatggcgac
      721 ccgctcggag atctggcgga cattcgagat ccgcgttccc gccgcaagga cggttgccga
      781 caggtccgcc tcgaatcgcc gggcgatggc gcgagccggg ggcggcggcc cgacacgccg
      841 gccggggcgg gactccacgg tcaccgctgg gaaccgaagg tggatcgggt ggaccacgcg
      901 ctcatgaccg cgaggatatc tcgatcccgc gggtccgtgc acattcgcac aggccagccc
      961 gcgtgagcgt ggaatcgccc gctacgctgc ggaggatgac ctcgtctgcc ttcgtgttcc
     1021 ccggccaggg ctcgcagtcg gtcggcatgg gacaggcgct cgccgctgca tccccggctg
     1081 ccgcctcggt cctcgccgcc gccgacgccg ccctcggcga atcgatcagc cgcctcgcct
     1141 tcgacgggcc cgccgacctc ctcgatcgga cggagaatgc ccagccggcg ctgctcgcgg
     1201 tgtcgatcgc gtacctcgcg gcgctgcgcg aacgatgggc cgccctgggc atcgaggcgc
     1261 ccgtgcccgc cttcgccgcc ggccactcga tgggccagta ctcggccctc gtcgcggccg
     1321 aggtgatctc gatcgaggat ggggtccgcc tggtccgcga gcgtggccgg ctgatgcagg
     1381 cctccggcgc cggccgcgac ggcgcgatgg cggcgatcct cggtctcgac gacgcagccc
     1441 tgccggcgct cgtcgagcga gcgtccgccg ccggcgtgtt cggcgtggcg aatcgcaacg
     1501 ctcccggcca ggtcgtcgtg tcgggtgagc gtgcggcgat cgaggtggcc gcgtcgatcg
     1561 cgaaggagct cggagcacga aaggccatcg tcctgccggt ctcggtcgcc gcccactcgc
     1621 cattgatgag cgaagcggcc gagggcatgc ggcgcgccct cgccgacgtg tcattccacg
     1681 acccggtcgt gccgctcctc gccaacgccg atgcccggcc gatcacgacc gccgacgacg
     1741 cccgggcgga gctcgtcgag cacctcaccg ccggggtcga ctgggtggcc gccgtggagc
     1801 ggatgacggc cgcgggggtg accacgttca tcgaggtcgg gcccgggcgc gtcctgaccg
     1861 gcttgatcaa gcggatcgca cccgacgccg acgtcctcgc tctcgatgat cccggctcgc
     1921 cggatcgact gaccgtcccg ttcgccatgg ccacaaccgc ctgacccccc gtttccagaa
     1981 aggattcctc gagcgtgcgc aaacccgatt acgaccgacg cgtcgtcgtc actggcctcg
     2041 gtgtcatcag cccggtcggc aacgatgtcc agaccgcctg gggaaacctg gtccgtggcc
     2101 agtccggcct gaccgagatc acgaagttcg acgtgtcccg ctacgaggcg aaggcggccg
     2161 gcgaggtcca cgatttcaac ccgaccgact ggatggaccc caaggcggtc cgccgcagcg
     2221 agagcgcgat gtggttcggc gtcgcggccg gcaagcaggc cctcgccgat tccggcttcg
     2281 agatcaccga cgccaaccgc gaggaggtcg gggtcgtctt cggctcgggc gcgggcggcc
     2341 agcagctgat gatcgacgcc tggatcaacc tccacgagaa gggtccgcgg agcgtcccgc
     2401 cgaccttcat cgccaacgcc ctcgtcgatt cggcgtcggg catgatcgcg atcgagacgg
     2461 gcgcgatcgg ccacaacgtg tgcatcgtgt cggcctgcgc gacgggaacc cacaacgtct
     2521 ccgagggcgc cgaggcgatc cgccgcgggg actgcatcgc ggtcatctcc ggctcgaccg
     2581 aggcaccgct cctcgagacg gcccacgccg ggttctcgaa catgcgaggg atgggcatgc
     2641 cccggcccgg cgaggggctc gagagcgtct cccggccgtt cgacaagacc cgcgacgggt
     2701 tcgtcctcgg cgagggcgcc ggtggcctgt tcctcgagga cctcgagctg gccaaggcgc
     2761 gcggcgcgag gatctacgcc gaggtcgtcg gctacggctc ggcggccgac ggctgggaca
     2821 tgatccagcc gatcgcgggc ggttcgggtt cggcgcgggc gatgaagatg gccctcgcgc
     2881 ggcgcggcgt cccggccgac gaggtcgacc tgatcaaccc ccacgggaca tcgacgccgc
     2941 tcggcgacca gcgcgaggcg gaggcgatct ggacggtctt cggcgatcgc gccgcagcgg
     3001 cgcgccactc gctggcgatc agcgggacga agtcgatgac cggtcacatg atgggcgcgg
     3061 ccggtgcctt cgaggcgttc gcgaccgtga tgtcggtcgc cgagcagtgc atcccgccga
     3121 cgatcaacta ccgcgacttc gaccccgtct gcgacctgtg ggtcgtgacc gaggcgacgc
     3181 cgcgagagat ccgctacggg ctgagcaaca acatcggcct cggcggccac aacggcgctg
     3241 tgatcttcaa gcgttacgac ggcgactgac ctcggcgggc gcgttgtgcg ccccggatca
     3301 cgtggcgccg cccggatcac gtggaggatg gcaccgctcg accgctcgac cgctcgaccg
     3361 cgcatccgcg acgatcgcct acgggtcagt acaatccgac gagcctgagc cctgaggtag
     3421 accttcgtgc ctgaactcga tccatcacgg cgtgcggtcg tcaccggcct gggggcggtc
     3481 atgccgatcg gcaacgactt cccgacctac tggtcgaacc tcgtcgccgg cgtcacgggg
     3541 acgcgtctca tccggagctt cgacgcgtcc gcgttcgagg tccggatcgc ggccgaagtg
     3601 ctcgatttcg acccgacgac cgtcatggac gcgaagatgg cccgccggat gacccgcttc
     3661 atccatctcg ggatgggcgc cgcaaaggaa gccgtggcca acagcgggat cgacttcgcc
     3721 gcgatgaccg aggacgagcg cgaccgggtc ggtgtcgtca tgaacaccgg cggcggtggg
     3781 atcgaggcga tcatcgacgg gactcacgtc cacgatcaga agggcccccg cttcgtgtcc
     3841 gcgttcgccg tgcccgcact gtcgggctcg atgggcgccg cgctgctgtc gatggagtgg
     3901 cacctgaccg gcccggtcat gacccaggtc gcagcctgcg cgacatcggt cattgccttc
     3961 cacgacgccc tccggctcat ccggaccggc gagtgcgacg tggtcctgac cggtggctcc
     4021 gaggcgcctg tcgtgccgat gggggtcgcg gccctggcca acatgggcgc cctctcgaag
     4081 cgcaacgact cgccggagac ggcgtcccga ccgttcgacg gcacgcgcga cggattcgtc
     4141 ctcggcgagg gcgccggtct ggtggtgatc gagtcgctcg cccacgctct cgcccgtggc
     4201 gcaacgccga tcgccgaggt tctcggaggg gccctgactg ccgatgcctt ccatatcagc
     4261 gcgcccgagc cgaccggccg cgccgacgag gtgaccgatc atcgacttcg gcgagctgat
     4321 cgcgaccttc cgggcatgag ccccgtaggc cgccttgatc gcccgtgtct cggtcacgtc
     4381 gttgagggat gtcgacgttc cgtgggcgac gatgtagtcg atctcgtcgg gggcgacgtc
     4441 gctgttgcgg agcgcgttcg tcatcgcccg ggtctggccg cggccggtcg gctcgggcgc
     4501 gctgatatgg aaggcatcgg cggtcagggc ccctccgaga acctcggcga tcggcgttgc
     4561 gccacgggcg agagc
//
LOCUS       DATAIW010000042         9351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_61081, whole genome shotgun sequence.
ACCESSION   DATAIW010000042 DATAIW010000000
VERSION     DATAIW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61081"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VHM48_01530"
     CDS             complement(<1..1411)
                     /locus_tag="VHM48_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2754110.1"
                     /translation="MDHDAVQAGGPGISDEGGGAGAAGTTAAATAGAAGPETLLDLLD
                     DACRRFADRPALGLWQDDGTRTTWTYRELDRRSRLAAWRLREQLHLQPGDRILTWSPS
                     EPALPAVYLGAMRAGLILVPLDLRMSPDAITGIIAMAEPRRLILGTGRDAPDPAPAGL
                     AAFPTTTIRDLVAEPDDTMPGTWAAEVERWPRPRPADIWDLIFTSGTTGRPKGVMVAH
                     DNLLATLGAIRHVLPPIEHRVVSVLPLSHLFEQAIGLIYALSVGADILYVRSRNPRVL
                     FEALRAHRVTSMIAVPQVLDLFWSAIEREADRSGQRARFDRLRGIARHLPYPVRRVLF
                     RRVHRRLGGGMRLFVSSGAFLPPALQQAWEDLGIVVVQGYGSSENGFGTCTTPQDHGL
                     GTVGRAMPPVELRVADDGEILFRGPTLFKGYWRDPETTARAIDADGWYHTGDIGHLDA
                     DGRLIMSGRTKDRIVLPNGF"
     gene            complement(1564..1902)
                     /locus_tag="VHM48_01535"
     CDS             complement(1564..1902)
                     /locus_tag="VHM48_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754111.1"
                     /translation="MKHRLAVGNDGLDLDDHDPAGLSMPGKDVDRTTFPENRERHLDR
                     HLPAVLLEQFHGSLDETCVRRVEKSIELFATPAQSDVQSSVQGRRDPDERHEGNLTRT
                     AALDGRDQAP"
     gene            2529..2714
                     /gene="rpsU"
                     /locus_tag="VHM48_01540"
     CDS             2529..2714
                     /gene="rpsU"
                     /locus_tag="VHM48_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX2754112.1"
                     /translation="MAEVRVGENETFESALRRFNKKIQQSGILAEARRREHYEKPSVK
                     RKRKEAKRKKNARTPAG"
     gene            2815..3306
                     /locus_tag="VHM48_01545"
     CDS             2815..3306
                     /locus_tag="VHM48_01545"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2754113.1"
                     /translation="MSLRDRIQADVTAAMRSGDSLRRDVLRMAQNAIYTVEKRDRREL
                     VDDDVTIILTREVKTRRESVEAFRKGAREDLVAKEEAEIAILTEYLPEPLDDASIQAL
                     VGEAVAATGASSARDLGKVMGWLAPKTRGRADGRRVSELVAQALARVDLAGHDTTGSH
                     GAG"
     gene            3318..5474
                     /locus_tag="VHM48_01550"
     CDS             3318..5474
                     /locus_tag="VHM48_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2754114.1"
                     /translation="MLTQRGIVNSSTFTRRDLGRLFTGAILLVAAMTAIFALDLVPHR
                     LDIAVGDVAAEDIVAPRTQSYVSAIQTAAKRDAARKTVDNVYDWSPDKAAGIAVAQAA
                     AFEDLVKPVDAAFKAGVKPADRVRMLSASLPLLVGPVRQTLADLDADRWKAVRQEASR
                     VLDLTEHSQVRDTDVATVQSKLAVQMVLEGAERTLAAAIISPLIEANSSFSSDLTNQA
                     RQDAADQVADVVVPYAQNETIVRHGEKVTAEALEAIVEFGLIDARPDVARLGGWFLLS
                     VLVVGILLGWIWRFRRQLWHRTNALVLIGLIVLGTTLLLKLTAGRHGLPFILPTAAAG
                     MLLAILLDAGVATIVLAMIALIGGAVNGPAGSLELMAYIFLGGMAGIVGIRRGDRLQI
                     FVQAGLAVAIVNVLVVAMYGFLGLHDARGVIELMGASLLSAAGSAVAAVGTFAVLGSV
                     FGILTVFQLLELANPSQPLLRRLLVETPGTYHHSLMVGNLAERSAEAIGADPLVTRVA
                     AYYHDIGKLANPLAFIENQAGGENVHDQLDPETSAQILKQHTADGIDIAYRAKLPKSL
                     IAFIPQHHGTAIMSYFYARAKGNAAEPYGGPQTADGARAAATVDERKFRHAGPKPQSR
                     EAALIMLADGVEASVRSLASRDEPAIRAMVARIIDERLSDGQFDECDLTLRDVERIKA
                     AFVQQLLGMYHQRIAYPQNKVVELESRRGTGTGTGA"
     gene            5491..6069
                     /gene="ybeY"
                     /locus_tag="VHM48_01555"
     CDS             5491..6069
                     /gene="ybeY"
                     /locus_tag="VHM48_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2754115.1"
                     /translation="MTAVYLAPWRVDVAIRDAVTDAHGGRRPIAVAPLARAVAAALDT
                     AGAPSPASIGLILSDDHELADLNEAHLGHEGPTDVLSFPLLPPEAFPPHVGGPDRGAT
                     PHLGDAAFFAGPPGQRIHLGDIVVSVERAAEQAAAGHGGQTGDVRWSTADELCLLVTH
                     GTLHVCGWDHADPAEEREMRDLEQRLLNGGSG"
     gene            complement(6329..8662)
                     /locus_tag="VHM48_01560"
     CDS             complement(6329..8662)
                     /locus_tag="VHM48_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754116.1"
                     /translation="MPHRITRVGSGRPSRSYHLDHRTRNARSRRGALVVLAGLAMVVS
                     GLFSPVAARSSTSASSVMPHAARVARGVDGRAVGAGLRVDAKQAARIAVGVPRTTRAL
                     QRHTVDLGARPSTKALAPSTKLALGGGTAAAKVGPSPLIPAPTLEQQVATSFPGIAEA
                     GTCSCEPPDPWIAVSPTYVVQSTNGMVRISNRAGTPLVSMPAWALFAVPVDRGDADPR
                     ILWDAVHGRWVGVITTFNGDFSANGLRLAISETADPTAAWIVYPIETGTYLADYPGIS
                     SSSDKIVLSSDDFSFSPFPVFQGGTIYVADWSNVIAGTSLYVGANYFDDTAAHFRPAQ
                     MLSSGSNVPVIFEFAGATPAYWEVTGLAHTFTYVAASDLNNDFGSALFTIPVNQPGQP
                     GPDTIANAVDERPTDAVYRSGQLWFVATGDYFDGVNHWTAARYTQVLTNANGALPTDA
                     GDLITQVGTHYFMPGVGISGNGSAFLLATRASASENPTSVVALVVPGVGIADYIDIEA
                     STQAYDGERWGDYVGVAADPSGSGAVWVAHELAAAGGGWRTSVARIVSDDAAPGAPGV
                     VAQAQITSSTLGATVPVKTSWGAATDAGSGVAAYLVERSDDGGGYFGVKTTSTSVTAP
                     LLVGHTYRYRVTAFDAVGNVGAPTYGPLYRPTLYQSNTSATVYTTGWGSSSSSSFSGG
                     SAKYSSTAGKYATFTATLARSISIIATKAASRGSFKVYVDGVFKGTISTYSTTTKYRQ
                     LVYQFSWSAPGTHKIKIVMSGTAGHPRVDLDAFVVLR"
     gene            8878..>9351
                     /gene="pth"
                     /locus_tag="VHM48_01565"
     CDS             8878..>9351
                     /gene="pth"
                     /locus_tag="VHM48_01565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX2754117.1"
                     /translation="MKIVVGLGNPGAQYEKTRHNIGWMVLDRIADRAGWTGRGRTRDA
                     AAIAMGRFRGLDLTIVKPLTFMNDSGMAVRKVLAREHAPLGDLLIVADDFALPFGKLR
                     FRENGGPGGHNGLGSIIDELRTEKFSRLRVGIGAPERGFVDHVLAPFEPDERQRLD"
BASE COUNT         1392 a         3219 c         3367 g         1373 t
ORIGIN      
        1 tgaagccgtt cggcaggacg atccgatcct tcgtccggcc gctcatgatc agtcgtccgt
       61 cggcgtcgag gtggccgatg tcgccggtgt ggtaccagcc gtcggcgtcg atcgcgcgcg
      121 ccgtcgtctc ggggtcgcgc cagtagccct tgaacagggt cggcccgcgg aagaggatct
      181 cgccgtcgtc ggcgacccgg agctcgaccg gcggcatcgc ccggccgacc gtcccgagcc
      241 cgtgatcctg cggtgtcgtg caggtcccga agccgttctc gctgctgccg tagccctgga
      301 caacgacgat tccgaggtcc tcccaggcct gttgaagggc cggcggcagg aacgcgccgg
      361 acgagacgaa gagacgcatc ccgccgccga gccggcggtg gaccctccgg aagagcacgc
      421 gccggaccgg gtaggggagg tggcgggcga tgccacgcag gcgatcgaag cgggcccgtt
      481 gtccggatcg atcggcctcg cgctcgatcg cgctccagaa caggtcgagc acctgtggca
      541 cggcgatcat gctcgtgacc cggtgggctc gaagggcttc gaagaggacg cgcgggttgc
      601 ggctccggac atagaggatg tcggcgccga cggacagcgc gtagatcagg ccgatcgcct
      661 gctcgaacag gtggctgagc gggagcaccg agacgacccg atgctcgatc ggcgggagca
      721 cgtggcggat cgccccgagc gtcgcgagga ggttgtcatg ggcgaccatc acccctttcg
      781 gcctgccggt cgtgcccgag gtgaagatca ggtcccagat gtcagcggga cgcggtcgtg
      841 gccagcgctc gacttcggcc gcccaggtgc ccggcatcgt gtcgtccggc tcggcgacga
      901 gatcccggat cgtggtcgtc gggaaggcgg cgagtccggc gggcgccggg tcgggggcgt
      961 cgcggcccgt gccgaggatg agacggcgcg gctcggccat ggcaatgatt ccggtgatgg
     1021 cgtcggggct catccggagg tcgagcggga cgaggatcag gccggcccgc atcgccccga
     1081 gatagacggc gggcagggcc ggctccgatg gactccaggt caggatccga tcgccgggct
     1141 ggaggtgcag ctgttcgcgc aggcgccaag ccgcaagccg gctgcgtcga tcgagctcgc
     1201 gataggtcca cgtcgtccgc gtcccgtcgt cctgccacag cccgagcgcc ggccggtcag
     1261 caaatcgacg gcaggcatcg tcgaggagat cgagaagcgt ctccgggcct gccgcgccgg
     1321 cggtcgccgc ggcggtcgtt cccgcggcgc cagcgccgcc gccctcgtcg cttatgccgg
     1381 ggccgccggc ctgaactgcg tcgtgatcca tcgttgagcg atggtaaagg gcgcgatgag
     1441 cggcggtgaa gagcgcgggt tcatcctcca ctcgtggact ggattcgact cgggctgtca
     1501 taccgggccc tgcggatccg ccgaggctgg cggcagctcg atgtcgccga gcgcgccggc
     1561 gtgtcacgga gcctgatctc ggccatcgag cgcggccgtg cgggtgaggt tcccctcgtg
     1621 acgctcgtca ggatcgcggc ggccctggac gctcgattgg acgtcggact gcgctggcgt
     1681 ggcgaacagc tcgatcgact tctcgacgcg gcgcacgcac gtctcgtcga gcgagccgtg
     1741 gaactgttcc agaagtacgg ctgggaggtg gcggtcgagg tgtcgttcgc gattctcggg
     1801 gaacgtggtt cgatcgacgt ccttgcccgg catggagaga ccggcaggat cgtggtcgtc
     1861 gaggtcaagt ccgtcgttcc cgacagccag gcgatgcttc atgccctcga tcgcaaggtc
     1921 cgcctcgcgc cccggatcgt ggtcgaacga gggtgggccg tcgacggacc ggtcgcccgc
     1981 ctcctcgtga tcggagatgg atcgacggct cgccgtcgga ttgcggctct cgcggtcacc
     2041 tatgcgtcgg ccttgtccga tcgcggagcc gcagttcgtt cgtggctacg tcggcccgtc
     2101 ggccgccttt ccgggctgct gtttctccca tacgccagcg gaggtggcac tagaccgaca
     2161 acgaccggcg tcactcgcgt ccgtcgccct cgggggacgc caatggggcg cgattcctcg
     2221 ccatctgctc ctcctggtga cgtacgggaa gaaccgccgt cgccgctcag cccgcattct
     2281 ggctagtgcc accccggctg gcgtatggga aagaaggact cgggggcgcg caggggcgag
     2341 gcgggagtgg ccaacctccg ctcgagacgc gcgcggcgac agggccagct ggcacgattg
     2401 accgcctcgg ggcactcggc tatgctccgg cggccctgtc accccgtggt ggcgggcgat
     2461 ccgtgtcggc ggcggctccg cccgaccagg atggagacga gacctgagcc ggaggaggtg
     2521 aaccgggatt ggcagaagtc cgagtgggtg agaacgagac gttcgaaagc gccctgcgcc
     2581 gcttcaacaa gaagatccag cagtccggca tcctcgccga ggctcgccga cgcgagcact
     2641 acgagaagcc gagcgtgaag cgcaagcgca aggaagcaaa gcgcaagaag aacgcccgaa
     2701 cgccggccgg ctgagccgaa aggctccgca cgacccgcgc ccccagggcg ttccgcggcc
     2761 gtcgaccgct gacccggtca cgaacccgcg accccgcccg aggtagccat tccggtgtcc
     2821 cttcgagatc ggatccaggc cgacgtcacc gccgcgatgc ggtccggtga ttcgctgcgt
     2881 cgcgatgtcc tgcggatggc ccagaacgcc atctataccg tcgagaagcg cgaccgccgc
     2941 gagctcgtcg acgacgacgt cacgatcatt ctgacccgcg aggtgaagac ccgccgtgag
     3001 tccgtcgagg cgttccgcaa gggcgcccgc gaggatctcg tggccaagga ggaggcggag
     3061 atcgccatcc tcaccgagta cctgcccgag ccgctcgacg acgcctcgat ccaggcgctc
     3121 gtcggcgagg cggtcgcggc gaccggcgcg agcagcgctc gcgacctcgg gaaggtcatg
     3181 ggctggctgg cgccgaagac ccgcggccgg gccgacggtc ggcgtgtctc ggagctcgtg
     3241 gcccaggccc tcgcgcgtgt cgacctggct ggccacgaca cgaccggctc gcacggggcc
     3301 ggctgagcgc cacggccatg ctgacgcagc gagggatcgt caacagctcg accttcaccc
     3361 ggcgagacct cggccggctg ttcaccgggg ccatcctcct cgtggccgcg atgaccgcga
     3421 tcttcgccct cgacctggtg ccgcaccggc tcgacatcgc ggtcggcgac gtcgcggctg
     3481 aagacatcgt cgcgccgcgg acgcagagct atgtcagcgc catccagacg gccgccaaga
     3541 gggatgccgc gcgaaagacg gtcgacaacg tctatgactg gtcgcccgac aaggccgccg
     3601 ggatcgccgt cgcccaggct gccgccttcg aggacctcgt caaaccggtc gacgctgcgt
     3661 tcaaggcggg cgtgaagccg gccgaccggg tccggatgct ctccgccagc ctgccgctcc
     3721 tcgtcggtcc ggtgcgccag acgctcgccg acctcgatgc cgatcgctgg aaggccgtcc
     3781 ggcaggaggc gtcgcgcgtc ctcgacctca ccgagcactc ccaggtccgc gacacggacg
     3841 tcgcgaccgt ccagtcgaag ctggctgtac agatggtgct cgaaggggcc gagcggacgc
     3901 tcgcggccgc gatcatctcg ccgttgatcg aggccaactc ctcgttcagc tccgatctca
     3961 ccaaccaggc ccgccaggac gcggccgacc aggtcgcgga tgtcgtggtc ccgtacgccc
     4021 agaacgagac gatcgtccgc cacggcgaga aggtcacggc ggaggcgctc gaggcgatcg
     4081 tggagttcgg cctgatcgat gcccgccccg acgtcgcccg gctcggtggt tggttcctgc
     4141 tgtcggtcct ggtcgtcggg atcctgctcg gctggatctg gcggttccgg cgtcagctct
     4201 ggcaccgcac gaacgccctc gtcctgatcg gcctgatcgt gctcgggaca acgttgctgc
     4261 tgaagctcac cgcgggacgc cacggccttc cgttcatcct tccgaccgca gccgccggga
     4321 tgctcctcgc gatcctcctc gacgccgggg tcgcgacgat cgtgctcgcg atgatcgccc
     4381 tcatcggcgg ggccgtgaac gggccggccg ggtccctcga gctgatggcc tacatcttcc
     4441 tcggcggcat ggccgggatc gtcggcatcc gtcgcggcga ccgcctccag atcttcgtcc
     4501 aggccggact cgccgttgcg atcgtcaatg tcctcgtcgt cgcgatgtac gggttcctcg
     4561 gcctgcatga cgcccgcggc gtcatcgagc tgatgggggc ctcgctcctg tccgccgcgg
     4621 gctcggcggt cgcggccgtc gggacgttcg ccgtcctcgg gagcgtcttc gggatcctca
     4681 ccgtcttcca gctgctcgag ctcgccaacc cgtcccagcc gctgctgcgc cgtctgctcg
     4741 tcgagactcc gggcacctat caccattcgc tgatggtcgg caacctcgcc gagcgctccg
     4801 ccgaggcgat cggcgccgat ccgctggtga cccgcgtcgc ggcctactac cacgacatcg
     4861 gcaagctcgc gaacccgctc gccttcatcg agaaccaggc gggtggcgag aacgtccacg
     4921 accagctcga cccggagacc tcggcccaga tcctcaagca gcacaccgcc gacgggatcg
     4981 acatcgccta ccgggcgaag ctccccaaga gcctgatcgc gttcatccca cagcaccacg
     5041 ggacggcgat catgagctac ttctacgccc gggcgaaggg caacgccgcc gagccctacg
     5101 gcgggccgca gacggccgat ggagcgcgtg cggcggcgac cgtcgacgag cgcaagttcc
     5161 gccacgccgg cccgaagccg cagtcgcgcg aggcggcgct gatcatgctc gccgacgggg
     5221 tcgaggcatc cgtccggtcg ctggcgtcgc gcgacgaacc ggcgatccgg gcgatggtcg
     5281 cccggatcat cgacgagcgg ttgtccgacg gccagttcga cgaatgcgac ctgacgctcc
     5341 gcgacgtgga gcggatcaag gcggccttcg tccagcagct gctcgggatg taccaccagc
     5401 ggattgccta tccccagaac aaggtcgtcg agctcgagtc gcggcgcggg accgggaccg
     5461 gcacgggggc gtgaggcccg cgaggcgccc gtgaccgcgg tctacctcgc cccgtggcgg
     5521 gtcgacgtcg cgatccgcga cgccgtgacc gatgcccacg gtggacggcg cccgatcgcg
     5581 gtcgcgccac ttgcccgggc cgttgcggcg gctctcgata cggccggcgc gccgtcgccg
     5641 gcgtcgatcg ggctgatcct gtcggacgac cacgagctcg ccgatctcaa cgaggcgcac
     5701 ctcgggcacg agggcccgac cgacgtcctg tcattcccgc tcctcccgcc ggaggcattc
     5761 ccgccgcacg tcggtggtcc cgaccgcgga gcgacgcccc acctgggcga cgcggccttc
     5821 ttcgcggggc ctccggggca gcggatccac ctcggcgaca tcgtcgtgtc ggtcgagcgc
     5881 gccgccgagc aggccgcggc cggccacggc ggccagaccg gcgacgtccg ctggtcgacc
     5941 gccgacgagc tctgtctcct cgtgacccac ggcacgctcc acgtctgcgg ctgggatcac
     6001 gccgacccgg ccgaggagcg cgagatgcgc gacctggagc agaggctgct caacgggggt
     6061 agtgggtagc gcgcgaggcg gggccaccgc tggctggttg tccgaaatgc tcgaccgatc
     6121 gagctcgatc ggcatcgtgg ctgacatcgg cgtgggcgac ctggatgcgg ctcggtcacc
     6181 ctgtcggtag ccgcggcgac gcgggcgggt tcatccggga cgcccgacga acggcgccgg
     6241 acccggacca ggaccccgga cacgacgatg ccggcgcccg tcgggcgccg gcatcgaacc
     6301 gtggttccgg atggacgggg ccgtcgcctc agcggaggac gacgaacgcg tcgaggtcga
     6361 ccctcgggtg gccggcggtg ccggacatga cgatcttgat cttgtgggtg ccgggcgccg
     6421 accagctgaa ctggtagacg agctggcggt acttggtggt cgtggagtag gtgctgatcg
     6481 tgcccttgaa gacgccgtcg acgtaaacct tgaacgagcc tcggctcgcc gccttggtgg
     6541 cgatgatcga gatcgaacgg gcgagggtgg cggtgaaggt ggcgtacttc ccggcggtcg
     6601 acgagtactt cgcgctgcca ccggagaacg acgagctcga ggacgagccc cagccggttg
     6661 tgtagacggt cgccgacgtg ttcgactgat agagcgtcgg gcgatagagc gggccgtagg
     6721 tcggggcgcc gacgttgccg accgcgtcga aggcggtgac ccggtagcgg taggtgtggc
     6781 cgacgagaag tggcgcagtg accgacgtgc tcgtggtctt gacgccgaag tagccgccac
     6841 cgtcgtccga gcgttcgacg aggtaggccg cgacgccgct gccggcatcg gtggcggcgc
     6901 cccagctcgt cttgaccggc acagtcgcac cgagcgtcga ggacgttatc tgcgcctggg
     6961 cgacgacacc gggggcgccg ggcgccgcgt cgtcggacac gatccgggca acgctggtcc
     7021 gccatccgcc gccggcggcg gcgagctcgt gggcgaccca gacggcaccg ctgccggacg
     7081 gatccgcggc gaccccgacg tagtcgcccc aacgctcgcc gtcataggcc tgcgtcgagg
     7141 cctcgatgtc gatgtaatcc gcgatgccca cgccgggcac gacgagggcg acgaccgagg
     7201 tcgggttctc gctcgccgac gcccgggtcg cgagcaggaa ggccgacccg ttgccgctga
     7261 tgccgacgcc cggcatgaag tagtgcgtgc cgacctgcgt gatcaggtcg cccgcgtcgg
     7321 tcgggagggc gccgttggcg ttcgtcagga cctgcgtgta gcgggccgcc gtccagtggt
     7381 tgacgccatc gaaatagtcg ccggtcgcga cgaaccagag ctgaccgctg cgatagaccg
     7441 cgtcggtcgg ccgctcgtcg accgcgttgg cgatcgtgtc cgggcccggc tggcccggct
     7501 ggttcaccgg gatcgtgaac agggcgctgc cgaagtcatt gttgagatcg gaagcggcga
     7561 catacgtgaa ggtgtgggcg aggccggtga cttcccaata ggccggggtc gcgccggcga
     7621 attcgaagat gaccgggacg ttcgagcccg acgagagcat ctgggccggc cggaagtgcg
     7681 ccgcggtgtc gtcgaagtag ttggccccga cgtagagcga cgtccccgcg atcacgttgg
     7741 accagtcggc gacgtagatg gtgccgccct ggaagaccgg gaacgggctg aaggagaagt
     7801 cgtcggagct caggacgatc ttgtcggacg acgacgagat ccccgggtaa tccgccaggt
     7861 aggtgccggt ctcgatcggg tagacgatcc aggcggccgt cgggtcggcg gtctcggaga
     7921 tcgccagccg gagcccgttg gcgctgaagt caccgttgaa ggtcgtgatg accccgaccc
     7981 agcggccgtg gaccgcgtcc cacaggattc gcggatccgc gtcgccgcgg tcgaccggca
     8041 ccgcgaacag ggcccacgcc ggcatcgaca cgagcggcgt gccggcacgg ttcgagatgc
     8101 ggaccatccc gttcgtcgac tggacgacgt aggtcgggct cactgcgatc cacggatccg
     8161 gcggctcgca gctgcacgtg cccgcctcgg cgatgccggg gaacgacgtg gccacctgct
     8221 gctcgagcgt cggcgcgggg atcaggggcg agggtccgac cttggctgcg gccgtcccgc
     8281 cgcccagcgc gagcttcgtg ctcggagcga gggccttcgt ggacgggcga gccccgaggt
     8341 cgaccgtgtg acgctggagc gcacgcgtcg tgcgcgggac gccgacggcg atgcgggcgg
     8401 cctgcttcgc gtcgacgcga agaccggctc cgacggcccg gccgtcgacg ccgcgcgcaa
     8461 cgcgggctgc gtggggcatg acgctcgacg ccgacgtcga cgatcgagcc gcgacgggac
     8521 tgaacagacc ggacacgacc atcgccaggc cggcgagcac cacgagcgcg ccacgccggg
     8581 agcgcgcgtt ccgcgtccgg tggtcgagat ggtaggagcg cgatggtcgg cctgagccga
     8641 cccgagtgat ccgatggggc atcagccctc tcctcccgcg gcctcccggc atcctggtac
     8701 ggacaccccc caatgaagcc ggtgccgatc ttcgcagatc cggccggctc gtggcgggtc
     8761 tgccgcgcaa atgtggtcgc tggtaccatg cctgcgggcc ctcccgggac gggaacgggc
     8821 ccgttcggcg tggtgtccac gccagcggac gggcgtcctg gggcgccggg agagcacgtg
     8881 aagatcgtcg tcggcctggg caatccgggc gcgcaatacg agaagacccg ccacaacatc
     8941 ggctggatgg tcctcgaccg catcgccgac cgggccggct ggacgggcag gggccggacg
     9001 cgcgacgccg ccgcgatcgc gatgggccgc ttccgcggtc tcgacctcac gatcgtcaag
     9061 ccgctgacct tcatgaacga ctccggcatg gccgttcgca aggtcctcgc ccgggagcac
     9121 gctccgctcg gcgacctgct catcgtggcc gacgacttcg ccctgccgtt cggcaagctg
     9181 cgcttccgcg agaacggtgg tcccggcggc cacaacggtc tcggctcgat catcgacgag
     9241 ctccggaccg agaagttcag ccgactccgt gtcgggatcg gcgcgccgga acgaggcttc
     9301 gtcgaccacg tcctcgcccc gttcgagccg gatgagcggc agcgcctcga c
//
LOCUS       DATAIW010000043         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_209188, whole genome shotgun sequence.
ACCESSION   DATAIW010000043 DATAIW010000000
VERSION     DATAIW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209188"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..751)
                     /locus_tag="VHM48_01570"
     CDS             complement(113..751)
                     /locus_tag="VHM48_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2754118.1"
                     /translation="MTAEPSAVADRPSAVVFDLGGVLIDWNPRYLYRRLIADEASMER
                     FLADVVSPAWNREMDRGRPLADLVAELVGRHPEQADLIEAYRTRWLEMLGAAYDDTVD
                     VLAELRTDGVRLLALTNWSAETFSLARPRYPFLTWFEDVVVSGEVGLIKPDPAIFRLL
                     ADRHGLEPSRTVFIDDASANVEAAAGLGFRAIRFTAASALRRELAAMGLLEG"
     gene            880..1335
                     /locus_tag="VHM48_01575"
     CDS             880..1335
                     /locus_tag="VHM48_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HEX2754119.1"
                     /translation="MDDATFWSLIDRFDWSKDEDDDIIEPAVMALALLPDSQIAQFQQ
                     ILARKLYALDGRAWARESGELIWFGDPDRVAVDGFLYARCLVVANGREFYDAVLADPA
                     TMPKDADFEAILMLAADAYDRKTGLVWEELDDTDVSYETFSNEAGWPAI"
     gene            1434..1952
                     /locus_tag="VHM48_01580"
     CDS             1434..1952
                     /locus_tag="VHM48_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2754120.1"
                     /translation="MFAAMSLIVRERDGGTLSWVASKPVSRSSILISKWLSASAVLWV
                     VAGLVPLGLTVATVTVLYGAPPVGAVFAAGFGIGAAISLFVAVALAASTFSASQPAVA
                     GITFAAFLLPTIVAAVIPFDIAPYLPTSILSWAMGVGTGAAVGFVTPIAWVLGLAAIG
                     LVGARRMAALEL"
     gene            2080..3339
                     /locus_tag="VHM48_01585"
     CDS             2080..3339
                     /locus_tag="VHM48_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754121.1"
                     /translation="MIAQRTGPLLRDPAFGRLWFIQASNQVGGNMALYALTILVFATT
                     RSNAAVSALVMSFLIPTILLSAVAGVLVDRLDVRFALIVPNTVRTLLMLGLAMAGANV
                     AMLLILNLGISLTTVVLTPAEASMIPRIVPTAQLETAMGVFNLTLQASFALGFAFLGP
                     LLVTIAGPSFVLAVVVGFYAAATIATFGLPSAPPVPAAPGVRHRSLHEPIDQLREGLA
                     AISGNREVSRPLMHLAAAASLVGVIGVLGPNLAASLGLDPKHLIVVVLPLGLGVVAGV
                     VGLRQLGADIPRRRAAEGGLLAFGLLALGLAVVGPAGTAAGLPVIPLVVVLAFVAGSA
                     YAATTVSAQTALFENMPAGARGRIFGVLASIVSAASLVPILIAGPLADKISGPVVIAI
                     TAAGVLALAGWSALAFGPRSQDARTEP"
     gene            complement(3347..>4796)
                     /locus_tag="VHM48_01590"
     CDS             complement(3347..>4796)
                     /locus_tag="VHM48_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754122.1"
                     /translation="SSPATPFDVIKRPVDLTLSASPTDDVAGGGSTLTAKIRILDPIS
                     TDAVTGGIEFHDITGGGDVLVGTSPLSYVGSPDWNRATIDVSGLALGTHVYEARYAGS
                     TTLGSDMATTNVVIGPQFSYVSLEVTPNPVLNTAHATATVTLSTGRKGSGSGGALPAA
                     TGTITLKRVSTGATIGTAPVSGPGPYTIQVPIYPTGTVGLVAEYSGDANFDPSTGNQV
                     DLVVLTDVVDATGVGLGASTFYPVVDGYKDSVGIKGLRNEPISVEIRIYNSTNHLVRA
                     RSVASGTGAYSVYWNGRSTSGILQPGGTYRIVQTLTDAKSHHLVVSKTVAISRKKLYY
                     TTKTLTKYADAAASVGHYGGGIAYKYSDHSVRAYASGGWVGIGWQFTLPSATTYKSLS
                     VGIYGTSGTPIAHFGSQNFSLCSYSSTWSADCFDHMTAMRSTSAGNGWASRAVSPTYN
                     RYGHAVRVNFSQYSGSSHFYKVRVIVTYGILK"
BASE COUNT          657 a         1675 c         1671 g          793 t
ORIGIN      
        1 aacccgcccc gacccggcca cgagccgcgc gtcccgaagg aagagcgacc ccgccggtcc
       61 aaccgcgact gaccgaggct cgccgccggc cgaccggccg accgccatcg cgtcagcctt
      121 ccagcaggcc catcgccgcg agctcccggc gcagggccga ggcagccgtg aaccggatcg
      181 cccggaagcc gagtcccgcg gccgcctcga cgttcgcgct cgcatcgtcg atgaagacgg
      241 tccgcgatgg ctcgaggccg tggcgatcgg ccaggagccg gaagatcgcc ggatcgggct
      301 tgatcaggcc gacctcgccc gagacgacga cgtcctcgaa ccaggtgagg aacggatagc
      361 gcggcctggc cagcgagaag gtctcggccg accagttcgt cagcgccaac aggcgaaccc
      421 cgtcggtccg cagctcggcg agcacgtcga cggtgtcgtc gtacgccgcg ccgagcattt
      481 ccagccagcg cgtccggtag gcctcgatca gatcggcctg ctcgggatgc cgtccgacga
      541 gctccgcgac caggtcggcg aggggccggc cgcggtccat ctcgcggttc cacgccgggc
      601 tcacgacgtc ggcgaggaac cgctccatcg acgcctcgtc ggcgatcagc cggcggtaga
      661 gatagcgcgg gttccagtcg atcaggacgc cgccgaggtc gaagacgacg gcggacgggc
      721 ggtccgcgac ggcggatggt tcagctgtca tgtcccgagt gtcgcatcgg cgcgccgtcg
      781 gcgcctgtcg agtggcctgt cgcccgacgc ccggacccgt caccatggac gacgacgtcc
      841 aggcatcggc cgccgggccg atcgagtgga ggagcagcga tggacgacgc gacgttctgg
      901 agcctgatcg accggttcga ctggtcgaag gacgaggacg acgacatcat cgagcccgcg
      961 gtcatggccc tcgccctctt gccggactcg cagatcgcgc agttccagca gatcctggcc
     1021 cgcaagctct acgccctcga cggtcgggcg tgggcccgcg agagcggcga gctgatctgg
     1081 ttcggcgatc cggaccgcgt cgccgtcgac gggttcctgt acgcccgctg cctcgtcgtg
     1141 gcgaacggcc gcgagttcta cgacgcggtc ctcgccgacc cggcgacgat gccgaaggac
     1201 gccgacttcg aggcgatcct catgctcgcg gccgatgcct acgaccgcaa gacggggctc
     1261 gtctgggagg aactcgacga caccgacgtc tcctacgaga ccttctcgaa cgaggccggc
     1321 tggccggcga tctgacggcc gccagcgacg gacgcgccac cacggtcatc accctcgcgc
     1381 cgctggacaa cgtcggcgct gcggtcgctt cgcagatctt catcttcgcg acgatcttcg
     1441 cggcgatgag cctgatcgtc cgggagcggg acggcgggac cctctcatgg gtggcctcga
     1501 agcccgtttc gcgatcgagc atcctcatct ccaagtggct gtccgcatcg gccgtcctgt
     1561 gggtcgtcgc ggggctcgtc ccgctcggcc tgacggtggc cacggtgacc gtgctgtatg
     1621 gcgcgccccc ggtcggtgcg gtgtttgccg cggggttcgg gatcggcgcg gcgatctccc
     1681 tcttcgtcgc cgtggctctc gcggcgtcca cgttctctgc cagccagccc gcggtcgcgg
     1741 ggatcacgtt cgcggcgttc ctgctgccga cgatcgtcgc cgcggtcatc ccgttcgaca
     1801 tcgcccccta cctgcccacc tcgatcctgt cgtgggcgat gggcgtcggg acgggcgcgg
     1861 cggtcgggtt cgtgaccccg atcgcctggg tcctcggact ggcggcgatc ggcctggtcg
     1921 gagcgcgaag gatggccgcc ctggagctgt gaccgacccg ctgcaacgga cggccgccgg
     1981 cccacagggc ccgcggccgt tcctcgtttc tgacggacga ctgcaacatc gacacggatt
     2041 cggccggtgt cccggcacgc gcggctgcag aatggggctg tgatcgccca acgcaccggc
     2101 ccgctcctgc gcgacccggc gttcgggcgg ctctggttca tccaggcgtc gaaccaggtc
     2161 ggcggcaaca tggccctgta cgcgctgacg atcctcgtct ttgcgacgac gcgatcgaac
     2221 gcggcggtca gcgccctcgt gatgtcattc ctgatcccga cgatcctgct gtcggcggtg
     2281 gccggggtcc tcgtcgatcg tctcgacgtc cgctttgcgc tcatcgtccc gaacaccgtc
     2341 cggacgctcc tgatgctcgg cctcgcgatg gcgggggcca acgtcgcgat gctcctgatc
     2401 ctcaacctcg ggatcagcct gacgacggtc gttctgaccc cggccgaggc gtcgatgatc
     2461 ccgcggatcg tgccgacggc ccagctcgag accgcgatgg gcgtcttcaa cctgaccctg
     2521 caggcgtcgt tcgcccttgg gtttgcgttc ctcgggccgc ttctcgtgac gatcgcgggt
     2581 ccgtcgttcg tgctcgccgt cgtcgtcggc ttctacgccg cggcgacgat cgcgaccttc
     2641 ggcctcccgt cggccccgcc cgtcccggcg gcgccggggg tccggcaccg cagcctccat
     2701 gagccgatcg accagctccg tgagggactc gcggcgatca gcggcaaccg cgaggtcagc
     2761 cggccgctga tgcacctcgc cgccgccgcg tccctcgtcg gcgtcatcgg cgtgctcggt
     2821 ccgaatctcg cggcgtcgct cggcctcgat ccgaagcacc tgatcgtcgt cgtcctgccg
     2881 ctcgggctgg gcgtcgtggc cggggtcgtc gggctccgtc agctcggcgc ggacatccct
     2941 cgtcgccgcg cggccgaagg cgggctgctc gcgttcggac tcctcgcact cggactggcc
     3001 gtggtgggac cggccgggac cgccgccggt ctgccggtca tcccgctcgt cgtcgtcctc
     3061 gccttcgtcg cgggctcggc gtacgccgcc acgaccgtgt cggcccagac ggcgctgttc
     3121 gagaacatgc cggccggtgc gcgcggtcgg atcttcggcg tgcttgcctc gatcgtcagc
     3181 gctgccagcc tggtcccgat cctcatcgcc ggtccgcttg cggacaagat cagcggaccg
     3241 gtggtgatcg cgatcacggc ggccggcgtg ctcgcgcttg ccggctggtc ggcgctcgcg
     3301 ttcgggccgc gcagtcagga cgcccggacc gaaccgtgac cgacggctat ttcaggatgc
     3361 cgtaggtgac gatcacccgg accttgtaga aatggctcga tccgctgtac tggctgaagt
     3421 tgacccggac ggcgtggccg tagcggttgt aggtcggcga gaccgcccgc gacgcccagc
     3481 cgttcccggc cgacgtcgat cgcatcgccg tcatgtggtc gaagcagtcc gcggaccagg
     3541 tcgacgagta gctgcacaac gagaagttct gcgagccgaa gtgggcgatg ggtgttccgg
     3601 acgtgccgta gatgccgacg ctcagcgact tgtaggtcgt cgcgctcggc agcgtgaact
     3661 gccagccgat cccgacccag ccgccggacg cgtaggcccg gacggaatga tcggagtact
     3721 tgtaggcgat gccgccgccg tagtgcccca ccgacgccgc ggcatccgcg tatttcgtca
     3781 gggtcttcgt cgtgtaatac agcttcttcc gcgagatcgc gacggtcttc gagacgacaa
     3841 gatggtggct cttggcgtcg gtcagggtct ggacgatgcg ataggtgccg ccgggctgga
     3901 ggatgccgct cgtgctccgc ccgttccagt agacgctgta ggcgccggtg ccgctcgcga
     3961 cgctgcgcgc ccggaccagg tggttcgtcg agttgtagat ccggatctcc accgagatgg
     4021 gctcgttgcg gaggcccttg atcccgaccg agtccttgta accgtcgacg accgggtaga
     4081 acgtgcttgc gcccagcccg acgccggtgg cgtcgacgac gtcggtcagg acgacgaggt
     4141 cgacctggtt gccggtcgac ggatcgaagt tggcgtcacc ggagtactcc gcgaccagcc
     4201 cgaccgtgcc ggtcggatag atcgggacct ggatcgtgta gggaccgggt ccgctgacgg
     4261 gagcggtacc gatcgtcgcg ccggtcgaca cccgcttgag cgtgatcgtg ccagtagctg
     4321 ccgggagggc tccgcccgat ccactgccct tccggccggt ggacagggtc acggtggccg
     4381 tcgcgtgggc cgtgttgagg acggggttcg gggtgacctc gagagacacg tagctgaact
     4441 gcggcccgat cacgacgttg gtcgtggcca tgtcgctgcc gagcgtcgtc gatcccgcgt
     4501 agcgggcctc gtagacatgc gttcccaggg cgaggccgct gacatcgatc gtggcccgat
     4561 tccagtcggg gctgccgacg tacgacaacg ggctcgtgcc gaccaggacg tcgccgccgc
     4621 cggtgatgtc atggaactcg atgccgcccg tgaccgcatc ggtcgagatc ggatccagga
     4681 tccggatctt cgccgtcagc gtcgagccgc cgccggcgac atcgtcggtc ggggaggcag
     4741 acagggtcag atcgaccggg cgcttgatga cgtcgaacgg cgttgcgggc gacgac
//
LOCUS       DATAIW010000044         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_61140, whole genome shotgun sequence.
ACCESSION   DATAIW010000044 DATAIW010000000
VERSION     DATAIW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61140"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VHM48_01595"
     CDS             <1..1876
                     /locus_tag="VHM48_01595"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX2754123.1"
                     /translation="AGAAGAPAAADEASVPAFSSAADAPSTIAADPLPGTSPFEPAPS
                     LPATRVATVGEPAAVEAKRGRSGLRWALALVGVLIVAAVSLLIVSLVGGRPATSTAIG
                     YMPATTFSYTEVRIDLPGDQRPKLASFLKAFPGFSDQSAIDPKLDDIFDRLVRAASKD
                     KQSWTNDIKPWFGGQVAIGVGLPDSVSTSTMATSGANNSLVVVTITDRAKAIAWLTKS
                     TEGLTINRSTHGDADLFIPAINSDAVAVAINDKVMLAGTTTALNAAIDSGGKGTLDQN
                     VDVKAAIATLDKDYVMFGVVRIRAYAEAALKLMSVNQPGVLDKTQIDETTLALIPTWQ
                     AQSARFENDAIVASTVSPPWAIGYDSTNGPSDVLGHVPAKTILYFDLHDVGPTLNAII
                     AKFRVLEELKPAFAQFDQAVSLLGGSDAVFGWWGDTALVVTPLSDGTIGGGLVIHPRD
                     AALADRLFSTLNGFIAIGGGSTGVVTRSEDHNGTKITILDLSAVPGVSSTSFPPGFKP
                     EFAWATNADVAVMGYGSEFVKAVLDAGPGTSLGDDARFKGLLGRVGSENMGVSFLDIA
                     AFRGLLEPIAQAQSTSDQWAYYTKEIQPYLKPIDAVIGAVRKDGNLDRGTGAFTVTGP
                     "
     gene            1950..2207
                     /gene="rpsP"
                     /locus_tag="VHM48_01600"
     CDS             1950..2207
                     /gene="rpsP"
                     /locus_tag="VHM48_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2754124.1"
                     /translation="MAVRIRLTRVGATKQPTYRVVVADGRSARDSRAIEIIGHYNPRT
                     EPIELSIDAEKAKAWIAKGAQPSDTVARLLRSTGILPAEKK"
     gene            2215..2448
                     /locus_tag="VHM48_01605"
     CDS             2215..2448
                     /locus_tag="VHM48_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX2754125.1"
                     /translation="MAAAELVEYVAKSLVDDPDAVKVEVVRDQGGTVIELHVAEDDMG
                     KVIGRNGSVAKALRTLLKVTAARDGEPVSLEIL"
     gene            2457..3176
                     /gene="rimM"
                     /locus_tag="VHM48_01610"
     CDS             2457..3176
                     /gene="rimM"
                     /locus_tag="VHM48_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2754126.1"
                     /translation="MTESTPSPGSAATDRRLVVALVRGVHGLNGAVRVEILTDRPEQR
                     YAVGSVLHREGSDERLTVAWSSAVSDGPGWRLQFAEVHDRSAAEDLKGAYLEIDAGPD
                     AALPRGAYFWHEVVGATVTATDGTVLGTIQDVYRSGGAEVYVVSGGDYGEFDVPAVRD
                     FIRIFAPRRGEIVVDTEALDLALPKRRRAPDDPDRPRAPRRRAGSRRAKPTATAAASD
                     GQAREDGAGPDRPNPFDVLAD"
     gene            3195..3884
                     /gene="trmD"
                     /locus_tag="VHM48_01615"
     CDS             3195..3884
                     /gene="trmD"
                     /locus_tag="VHM48_01615"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2754127.1"
                     /translation="MALEIDVLTLFPEMVSGPLAASIPGRIQERGIATIRTHDLRQWG
                     IGRHRSVDDAPYGGGAGMVMRPEPIADGIDSVRRDDSLVILMDPGGEVFAQRRAHDLA
                     ERSHLVFVCARYEGVDERVRSLVNLELSIGDYVLTGGELPALVVIDTVIRLLPGAIDD
                     LSTTEESFADGLLEYPQYTRPPRFRGQDVPAILTSGDHGAVARWREEQARARTLRRRP
                     DLIEPPDDRRR"
BASE COUNT          631 a         1505 c         1362 g          581 t
ORIGIN      
        1 cgcgggcgcc gccggcgccc ccgcagccgc cgacgaagcg tcggttccgg cgttctcctc
       61 cgctgcggat gcgccctcga cgatcgccgc ggatccgctt ccggggacct cgcccttcga
      121 gccggcgccg agcctgcccg cgacccgggt cgcgacggtc ggcgagcccg cagcggtcga
      181 agccaaacgg ggtcggtccg gcctgcgctg ggcccttgcc cttgtcggcg tcctgatcgt
      241 cgccgcagtg tcgctcctga tcgtctccct tgtcggtggc cgaccggcga cgtcgacggc
      301 gatcggctac atgccggcga ccacgttcag ctacaccgaa gtgcggatcg acctgcccgg
      361 cgatcagcgc ccgaagctgg cgtccttcct gaaggccttc cccggcttca gcgaccagag
      421 tgcgatcgat ccgaagctcg atgacatctt cgatcggctc gtccgggcgg cgagcaagga
      481 caagcagagc tggaccaacg acatcaagcc gtggttcggc ggccaggtcg cgatcggcgt
      541 cgggctgccc gactccgtgt cgacttccac catggcgacg tcgggggcca acaacagcct
      601 cgtcgtcgtc acgatcacgg accgcgcgaa ggctatcgcc tggctgacca agagcaccga
      661 aggcctgacg atcaaccgct cgacgcacgg cgacgcggac ctcttcattc cggccatcaa
      721 cagcgacgcc gttgcggtcg ccatcaacga caaggtgatg ctcgccggca cgacgaccgc
      781 gctgaacgcc gccatcgact ccggtggcaa gggcacgctc gaccagaacg tcgatgtcaa
      841 ggcggccatc gccacgctcg acaaggacta cgtcatgttc ggcgtcgtcc ggatccgggc
      901 ctacgccgaa gcggccctca agctgatgtc cgtcaaccag cccggcgtcc tcgacaagac
      961 ccagatcgac gagacgacgc tcgcgctgat cccgacctgg caggcacagt cggcccgctt
     1021 cgagaacgac gccatcgtcg cctcgaccgt cagcccgccc tgggcgatcg gctacgactc
     1081 gacgaacggg cccagcgacg tgctcggcca cgtgccggcg aagacgatcc tctacttcga
     1141 cctgcacgac gtcgggccaa cgctgaacgc gatcatcgcc aagttccggg tgctggagga
     1201 attgaagccc gcgttcgccc agttcgacca ggcggtgagc ctcctcggcg gctccgacgc
     1261 ggtcttcggc tggtggggcg acacggccct ggttgtcacc ccgctctccg acggcacgat
     1321 cgggggcggt ctcgtcatcc acccgcgcga cgcggcgctg gccgaccggc tcttctcgac
     1381 cctcaacggg ttcatcgcga tcggcggtgg cagcaccggc gtcgtcaccc ggtccgagga
     1441 ccacaacggg acgaagatca cgatcctcga cctgagcgcg gtgcccggcg tgagcagcac
     1501 gagttttccg cccggcttca agcccgagtt cgcgtgggcg accaacgccg atgtggccgt
     1561 catgggctat ggcagcgagt tcgtcaaggc cgtgctcgat gcggggcccg gcacctcgct
     1621 cggcgatgac gcccggttca agggcctcct cggtcgggtc ggctccgaga acatgggcgt
     1681 ctcgttcctc gacatcgccg cgttccgcgg cctgctcgag ccgattgccc aggcccagtc
     1741 gacttctgac cagtgggcct actacaccaa ggagatccag ccctacctca agcccatcga
     1801 cgccgtgatc ggcgccgtcc gcaaggacgg caacctcgac cgcggcaccg gtgcattcac
     1861 agtcaccggg ccctgaggcg ccggcccgac atcgttcgac cagccggccc cccagaggcc
     1921 gacggagcac caggaggaat cagaaccaga tggcagtccg catccggctg acgcgcgttg
     1981 gcgcgacgaa gcagccgacc taccgcgtcg tcgttgccga tgggcgatcc gcccgcgaca
     2041 gccgggcgat cgagatcatc ggccactaca acccccgaac cgagccgatc gagctcagca
     2101 tcgacgccga gaaggccaag gcctggatcg ccaagggcgc ccagccgtcc gacacggtcg
     2161 cccgcctcct ccggtccacc ggaatcctcc ccgccgagaa gaagtaggac caagatggca
     2221 gcagccgaac tcgtcgaata cgtcgccaag tcgctcgtcg atgaccccga cgcggtcaag
     2281 gtcgaggtcg tccgcgacca gggcggcacc gtcatcgagc tccatgtcgc cgaagacgac
     2341 atgggcaagg tgatcggtcg caacggcagc gtcgccaaag cgctccggac cctgctcaag
     2401 gtgaccgcgg cccgcgacgg cgagcccgtc tccctcgaga tcctctaacg gcttccgtga
     2461 ccgagtcgac cccgtcgccc gggtcggcgg cgaccgaccg tcgtctcgtg gtcgcgctcg
     2521 tccgcggcgt tcacggcctg aacggcgccg tccgggtcga gatcctcacg gatcggcccg
     2581 agcagcgcta tgcggtcgga tcggtcctcc atcgggaggg ctcggatgag cgcctgaccg
     2641 tggcctggtc gtcggcggtc agcgacggcc cgggctggcg gctccagttc gccgaggtcc
     2701 acgaccgctc ggcggccgag gacctgaagg gcgcctacct cgagatcgac gccggtccgg
     2761 acgcggcgct gccccgtggc gcctacttct ggcacgaggt cgtcggggcg acggtgaccg
     2821 cgaccgacgg aaccgtcctc gggacgatcc aggacgtcta ccgctccggt ggcgccgagg
     2881 tctacgtcgt gtcgggcggc gactacggcg agttcgacgt cccggcggtc cgcgacttca
     2941 tccggatctt cgcgccgcgt cgcggcgaga tcgtggtcga caccgaagcc ctcgatctgg
     3001 ccttgccgaa gcgtcgccgc gccccggacg atccggaccg cccccgtgcg ccgcgccgac
     3061 gggcgggatc gcgccgggcg aagccgaccg cgaccgcagc cgcgagcgat gggcaggccc
     3121 gggaggacgg cgcgggaccc gatcgcccga acccgttcga cgtcctggcc gactgacggc
     3181 gcgccggccc accgatggcg ctcgagatcg acgtcctgac cctgttcccc gagatggtca
     3241 gcggtccgct cgcggccagc attcccggcc ggatccagga gcgcggcatc gccacgatcc
     3301 ggacccacga cctccgccag tgggggatcg gccggcatcg cagcgtcgac gacgcgccgt
     3361 acggcggcgg cgccgggatg gtgatgcgcc cggagccgat cgccgacggg atcgattcgg
     3421 tccggcggga cgactcgctc gtgatcctga tggaccccgg cggcgaggtc ttcgcccagc
     3481 gccgcgctca cgacctggcc gagcgatcgc acctcgtctt cgtctgcgcc cgctatgaag
     3541 gcgtcgacga gcgggtgcgc tcgctggtca acctggagct ctcgatcggc gactacgtcc
     3601 tgaccggtgg tgagctgccg gcccttgtcg tgatcgacac cgtgatccgc ctgctgccgg
     3661 gcgcgatcga tgacctctcg acgaccgagg agtcgttcgc cgatggcctg ctcgaatatc
     3721 cgcaatacac gcgaccaccg aggttccgcg ggcaggacgt gccggcgatc ctcacctccg
     3781 gcgaccacgg cgcggtcgcc cgctggcgcg aggagcaggc ccgcgcccgg accctgcggc
     3841 gacggcccga cctgatcgag ccgcccgacg atcgccgccg gtaggaccgt cggaatcgcg
     3901 aagccccggc cgggagccgg gcttctaacc gtctggaccg acaagggtct gcggccgtgc
     3961 accgtgaacg tgctctcgcg ttgccgggag gcgcggccga cgtcgacgcc tggtgaccgc
     4021 ggcgccgcgc gggcatctcc tccctgggcg tccgggcgca cgcggtcgcc atgctatac
//
LOCUS       DATAIW010000045         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1409, whole genome shotgun sequence.
ACCESSION   DATAIW010000045 DATAIW010000000
VERSION     DATAIW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VHM48_01620"
     CDS             <1..316
                     /locus_tag="VHM48_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2754128.1"
                     /translation="QILYGSGHDIEASGRAPGFHDLLGDVWRERGFGDFWGYALLAEG
                     AAEAMVEVDLSTWDAAAPMILVEEAGGRATDLAGRRAIDSRTFLVSNGVLHDEVLGRL
                     RG"
     gene            complement(318..890)
                     /locus_tag="VHM48_01625"
     CDS             complement(318..890)
                     /locus_tag="VHM48_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754129.1"
                     /translation="MTAAPIAPTSERVRTFLDRLERLSVDELSVLALAPFDPSEADRL
                     RQRAEVAAEDAGRLGEFDEAVDRARDLVVQAFSFRGLEPTWFGLNWGRSLSRAEDRAL
                     LFAAVQDAAMAIVVGDLLPDEAAALAEPYELAASMRGSAPTSNPASSTHRNAVRVAWV
                     LGAFGWITVGAQLLFDLVAEIVSRGSNLLR"
     gene            complement(887..1552)
                     /locus_tag="VHM48_01630"
     CDS             complement(887..1552)
                     /locus_tag="VHM48_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX2754130.1"
                     /translation="MSTDVAAVRAEVVRVARALRERGLAIGTSGNVGARLDDGRIAIT
                     PSTMDYEDMTPDDVMIVAADGTPSEGRHRPSSELRIHLEVFAARQDVAGIVHTHSPFA
                     TTWAAACRPLPAVHYILAMLVCPGRDTLRVASYATFGTVDLARNAVAALDRDNAVLLA
                     NHGAIAVGRTLASALGRAERVEELAMLAWRAEQIGGATLLDADELDRVRDQMARFPRQ
                     RSR"
     gene            complement(1549..2181)
                     /locus_tag="VHM48_01635"
     CDS             complement(1549..2181)
                     /locus_tag="VHM48_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754131.1"
                     /translation="MGERRGVGPGVRVVPATPDRWNDVVTLLGGGGDRGCWCQAPRGV
                     ARGYGTAEPGTRREALRVQVAEDDPPPGMLAYLDGAVAGWCGFGRRPDLPRLVRSRTI
                     PTIDDLPVWSILCFNVRVGYRRRGVAAALLDGVVDLARRSGAPGVEAYPIDPEGGRVD
                     TSFGYVGLTPMFEKVGFRRVVETTAHSDRRPRILMRLDFDPSSPNGGATR"
     gene            complement(2160..2480)
                     /locus_tag="VHM48_01640"
     CDS             complement(2160..2480)
                     /locus_tag="VHM48_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754132.1"
                     /translation="MFAVRRREPAHRRAVEAAELVAIETGREAAVQAARLIGAGYIER
                     RFSQGGFRTTLGLMPFASGISGPERAGLAVSFEEAVTGLVLADVLDEADVDELLGPWA
                     NVEE"
     gene            complement(2579..3904)
                     /locus_tag="VHM48_01645"
     CDS             complement(2579..3904)
                     /locus_tag="VHM48_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2754133.1"
                     /translation="MVDTAPAPTTSAPLPDRSVVPPVWGRVTNLIIERGEGSWLITTD
                     GERYLDYSSGIGVTNTGHAHPRVVAAVQEQAAKLLHGQQNILYHEAGLRLHERLRYVL
                     PGDGWGAFLSNSGAEAVEASVKLARIATGRPVILGFRYGYHGRTGQTMALTTAKDVYR
                     GHFEPLPGSVYHTAYPYCYRAPGGSHAADERCTCDWEEQLDLTFHQYVYPEHVAAIIV
                     EPILGEGGYIVPPPAFLPRLREITRQHGILLIADEVQTGFGRTGELFAVNHWGVEPDI
                     LVMAKGIASGLPLSGIVAQRAILDKLPAGSHGGTYGGNVVACAAANATLDVIEEEGLV
                     ANARERGDQFLAGLRAIAPKYASIGDVRGLGLMVALEFVKPGEGDGRVPNPDVTKRVQ
                     AEALARKLIILTAGTYVNVVRIIPPLVTTADEVDQALRILDESLAAAGA"
     gene            complement(3925..4818)
                     /locus_tag="VHM48_01650"
     CDS             complement(3925..4818)
                     /locus_tag="VHM48_01650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754134.1"
                     /translation="MADATTLQAGNRPAAAARRRSGGFRHGLQHRALPIFTTLAIVYL
                     MIPIAVMIVFSFNKPDGKFNYIWNTFSLEGWAHPFDWPGLGDAVITSLSVALISTIIA
                     TIIGTMMGLALTRYNFRGRGALNGIIFLPMATPEIVMGASLLTLFVATALPPLAGNVP
                     TLYPTGFLTILIAHVMFNISYVVVTVKARLAGFDRRLEEAAMDLGANEITTFWKVTFP
                     LIFPGIMAAALLAFSLSIDDFVITNFVSGRTNTFPIWVYSIQKNALPVQINVIGSIIF
                     LGAVTLVGLTTLRSGRAARIR"
     gene            complement(4818..5750)
                     /locus_tag="VHM48_01655"
     CDS             complement(4818..5750)
                     /locus_tag="VHM48_01655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754135.1"
                     /translation="MTAATASAPRGPTLRARFGRTVPYLLLAPGLLWLVIFYVIPAIQ
                     MFTYSISTGTLETGYVMTFNADAYATAIDRFGKQFLNSILFGGLATILTLAIGFPVAY
                     TIAFRGGRYKNLILFLVIAPFFTSFLIRTISWKILLGDEGPLLAPLRHGFGILANDWP
                     ILGTGALIDGRFSLINTPLAQVAGLTYNFLPFMILPLYVALEKIDIRLIEAAEDLYAN
                     RAAAFRRVTLPLALPGVFAGSLLTFIPSMGDYINAELLGNPGTRMIGNVIQNRLLVQN
                     DYPVGSALSFMLMAGILVAVAVYARLLGTEQLTG"
     gene            complement(5782..>6622)
                     /locus_tag="VHM48_01660"
     CDS             complement(5782..>6622)
                     /locus_tag="VHM48_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2754136.1"
                     /translation="GWDLVVLTDWMASKIVANKWAETIDQTNVPNCTANIRDALKNQT
                     WDPTNDYHYPWQSGMTGVGFNAKTLKANNIAEPTKIADLWNIPPEKLTFLAEARDTFG
                     LVLLKLGVDANGATVTADQLQQAADSIQPLADGGLRFTGNEYLQDFGQKKVWAAFVWS
                     GDLASSGGPDDKFIFPEEGTLIWTDNMLIPKGAANKYTAELMMNYVYDPKIAAQIANY
                     VYYVSPVKGADEEIKKLDAEAAANPLLFPTPEVVAKQHNFQSLSDDIEKKLNDLFSKL
                     SGT"
BASE COUNT         1048 a         2152 c         2392 g         1030 t
ORIGIN      
        1 ccagatcctg tacggctcgg gtcacgacat cgaggcgtcc ggtcgggcgc cgggctttca
       61 tgacctgctg ggcgatgtct ggcgcgagcg cggcttcggc gacttctggg gctacgcgct
      121 gctcgcggag ggcgccgccg aggcgatggt cgaggtcgac ctctcgacct gggacgcggc
      181 ggcgccgatg atcctcgtcg aggaggccgg cgggcgggcg accgacctcg ccggccgccg
      241 ggcgatcgac agccggacct tcctcgtctc gaacggcgtg ctccacgacg aggtcctggg
      301 gcgcctgcgc gggtgagcta gcgcagcagg ttcgatcccc ggctcacgat ctcggcgacc
      361 aggtcgaaca acagctgggc gccgactgtg atccagccga atgccccgag gacccacgcc
      421 acgcggacgg cgttccggtg ggtgctcgag gccggattgg acgtcggcgc ggacccgcgc
      481 atcgaggccg ccagctcgta cggctcggcg agagcagccg cctcgtcggg cagcaggtcc
      541 cccacgacga tggccatcgc tgcgtcctgg accgctgcga agagcagcgc ccggtcctcg
      601 gctcggctca gcgagcggcc ccagttcaat ccgaaccagg tcggttcgag gccgcggaac
      661 gagaacgcct ggacgaccag gtcgcgtgcc cggtcgacgg cctcgtcgaa ctcacccaga
      721 cgaccggcgt cctccgccgc gacctcagcg cgctggcgga gccgatccgc ctcggacgga
      781 tcgaatggcg cgagcgcgag cacgctgagc tcgtcgacgc tcagccgttc gagtcggtcg
      841 aggaacgtcc ggacgcgctc cgacgtcggc gcgatcggcg ctgcagtcat cgggatctct
      901 gtcgcggaaa ccgtgccatc tggtcgcgga cgcgatcgag ctcgtcggcg tcgagcaggg
      961 tcgcgccgcc gatctgctcc gcgcgccagg cgagcatcgc cagctcctcg acccgctccg
     1021 cgcgtccgag cgcggacgcc agcgtccggc cgaccgcgat cgcgccgtga ttggcgagaa
     1081 ggaccgcgtt gtcccggtcg agagccgcga cggcatttcg cgccagatcc accgtcccga
     1141 aggtcgcata ggacgcaacc cggagcgtgt cgcggccagg gcagacgagc atcgccagga
     1201 tgtagtggac cgcgggcagg ggacggcagg cggcggccca ggtcgtcgcg aacggcgaat
     1261 gggtatggac gatcccggcg acatcctgac gggcggcgaa cacctcgaga tggatccgca
     1321 gctcggagct cggtcggtga cggccctcgg acggggtgcc gtcggccgcg acgatcatga
     1381 cgtcgtcggg cgtcatgtcc tcgtagtcca tcgtcgaggg cgtgatcgcg atccggccgt
     1441 cgtcgaggcg ggccccgacg ttgcccgacg tcccgatcgc caggccgcgt tcacgcagcg
     1501 cgcgggcaac ccggacgacc tccgcccgga ccgcggcgac gtcggtgctc atcgggtggc
     1561 gcctccgttc ggggacgagg ggtcgaagtc caggcgcatg aggatccgcg gccgacgatc
     1621 gctgtgggcg gtcgtctcga ccacccgtcg gaatccgacc ttttcgaaca tcggggtcag
     1681 cccgacgtag ccgaagctcg tgtccacgcg acctccctcg ggatcgatcg gataggcctc
     1741 gacgccgggc gctccggatc gccgggccag atcgacgacg ccgtcgagca atgctgccgc
     1801 gacgcctcgc cggcgatacc cgacgcgaac attgaagcag aggatcgacc agaccggcag
     1861 gtcatcgatc gtcgggatcg tccgcgaccg gacgagacga ggcaggtctg gtcgccgtcc
     1921 gaagccgcac cagccggcga ccgcgccgtc gaggtaggcg agcatgcccg gcggcggatc
     1981 gtcctccgcg acctgcaccc gcagggcctc gcggcgggtt cccggctcgg cggttccgta
     2041 gccgcgcgcg acgccccgcg gcgcctggca ccagcagcca cgatcgccgc cgccaccgag
     2101 caacgtgacg acgtcgttcc agcggtcggg cgtcgcgggg acgacccgta cgcccgggcc
     2161 tactcctcga cgttcgccca cggcccgagt agctcgtcga catcggcttc gtcgagcacg
     2221 tcggccagga cgaggccggt caccgcttcc tcgaatgaaa ccgccaggcc ggcgcgctca
     2281 ggtccgctga ttccacttgc gaagggcatc aggccgaggg tggtccgaaa cccgccttgc
     2341 gagaatcgcc tctcgatgta cccggcgccg atcagccggg cggcctgaac ggcggcttct
     2401 cggccggtct cgatcgcgac cagctcggct gcctcgaccg cgcgtcgatg cgcgggttca
     2461 cgccgcctca cggcaaacat cggcaactgg tcgtaaccga cccggtcgac ggcttcgctg
     2521 aacctgtcga gccgacgtcg gatgtcgcgt ggcagctgat cgagcggacc ggaattcatc
     2581 atgccccggc ggcggcgagg ctctcgtcga ggatccgcag ggcctggtcg acttcatcgg
     2641 ccgtggtgac gaggggcgga atgatccgga cgacattcac gtaggtcccg gccgtcagga
     2701 tgatcagctt gcgagcgagc gcctccgcct ggacacgctt cgtgacgtcg gggttcggga
     2761 cgcggccgtc gccctcaccg ggcttcacga attcgagggc gaccatcaac cccaggccgc
     2821 ggacgtcgcc gatcgaggcg tacttcgggg cgatggcccg cagcccggcc aggaactggt
     2881 cgccgcgctc gcgggcgttg gcgacgagcc cctcctcctc gatgacatcg agcgttgcgt
     2941 tggccgcggc acaggcaacg acgttgccgc cgtacgtgcc gccgtgggat cccgccggga
     3001 gcttgtcgag gatcgcgcgc tgggcgacga tcccgctcag gggcaggccg gaggcgatcc
     3061 ccttggccat cacgaggatg tccggctcga cgccccaatg gttgaccgcg aacagctcgc
     3121 cggtccgccc gaacccggtc tggacctcat cggcgatcaa caggatcccg tgctggcggg
     3181 tgatctcgcg gagccgcggc aggaacgccg gcggcgggac gatgtagccg ccctcgccga
     3241 ggatcggctc gacgatgatc gccgcgacgt gctccgggta gacgtactgg tggaacgtca
     3301 ggtcgagctg ctcctcccag tcgcaggtgc agcgctcgtc ggcggcgtgc gacccgcctg
     3361 gcgcgcggta gcagtacggg taggcggtgt ggtacacgct gcccggcagc ggctcgaagt
     3421 ggccgcggta gacgtccttg gcggtcgtga gggccatcgt ctggccggtc cggccgtggt
     3481 agccgtagcg gaatccgagg atcaccggcc gaccggtcgc gatccgggcg agcttgaccg
     3541 atgcctcgac cgcctccgcc ccgctgttcg agaggaatgc gccccagccg tcgccgggca
     3601 ggacgtaacg gagtcgctcg tggagccgca gcccggcctc gtggtacagg atgttctgct
     3661 ggccgtggag gagcttggcg gcctgctcct ggaccgcggc gacgacgcgc gggtgagcgt
     3721 ggccggtgtt cgtgacgccg atccccgacg aatagtcgag atagcgctcg ccgtcggtcg
     3781 tgatcagcca cgatccttcg ccccgctcga tgatcaagtt cgtgacccgg ccccagaccg
     3841 ggggcacgac cgagcggtcg ggcaggggag cggacgtcgt cggagccggc gcggtatcga
     3901 ccatgggact gagggtctcc cggactagcg gatgcgggca gcgcggccgg accgcagggt
     3961 cgtgagcccg acgagcgtga cggcgccgag gaagatgatc gagccgatca cgttgatctg
     4021 gaccggcagg gcattcttct ggatcgaata gacccagatc gggaaggtgt tggttcgtcc
     4081 cgacacgaag ttggtgatga cgaagtcgtc gatcgacagg ctgaacgcga ggagcgcggc
     4141 tgccatgatg ccggggaaga tcagggggaa tgtcaccttc cagaaggtcg tgatctcgtt
     4201 cgccccgagg tccatcgcgg cctcctcgag ccgccggtcg aagccggcga ggcgggcctt
     4261 cacggtcacc acgacgtagc tgatgttgaa catcacgtgg gcgatcagga tggtcaggaa
     4321 gccggtcgga tagagggtcg ggacattgcc cgcgagcggc ggcagggcgg tcgccacgaa
     4381 cagcgtgagc aagctcgcgc ccatgacgat ctcgggcgtc gccatcggca ggaagatgat
     4441 cccgttgagg gcgccccggc cgcggaagtt gtagcgggtc agggcgaggc ccatcatcgt
     4501 cccgatgatc gtggcgatga tcgtcgagat cagggcgacc gacaggctcg tgatcacggc
     4561 gtcgccgaga ccgggccagt cgaacgggtg agcccagccc tcgagcgaga acgtgttcca
     4621 gatgtagttg aacttgccgt cgggcttgtt gaacgagaag acgatcatca ccgcgatcgg
     4681 gatcatcagg tacacgatcg caagggtcgt gaagatcggc aacgcccggt gctgcagacc
     4741 gtggcggaag ccgccggacc gcctgcgcgc ggcggccgcc ggccggttcc ctgcctggag
     4801 cgtcgtcgcg tcggccatca gccggtcagc tgctcggtgc cgagcagtcg cgcatacacc
     4861 gcgacggcga cgaggatgcc ggccatcagc atgaacgaca gggccgatcc aaccggatag
     4921 tcgttctgga cgaggagccg gttctggatg acgtttccga tcatccgggt gccggggttg
     4981 ccgaggagct ccgcgttgat gtagtcgccc atcgacggga tgaacgtcaa cagcgagccg
     5041 gcaaacaccc cggggagcgc gagcggcagg gtgacccgcc ggaacgccgc ggcccggttg
     5101 gcgtacaggt cctcggccgc ctcgatcagc cggatgtcga tcttctcgag cgcgacgtac
     5161 agcggcagga tcatgaacgg caggaagttg taggtcagtc cggcaacctg ggcgagcggc
     5221 gtgttgatca ggctgaaccg gccgtcgatc agggccccgg ttccgaggat cggccagtca
     5281 ttggccagga tcccgaagcc gtgccgaagg ggcgccagca ggggaccctc gtccccgagg
     5341 aggatcttcc agctgatcgt ccggatcagg aagctcgtga agaacggggc gatgaccagg
     5401 aacaggatca ggttcttgta gcggccgccg cggaaggcga tcgtgtacgc gaccgggaag
     5461 ccgatcgcga gggtcaggat cgtcgcgagg ccgccgaaca ggatcgagtt gaggaactgc
     5521 ttgccgaacc gatcgatcgc cgtcgcgtag gcatcggcgt tgaaggtcat gacgtagccc
     5581 gtctcgagcg tcccggtcga gatcgagtag gtgaacatct ggatggccgg gatgacgtag
     5641 aagatcacga gccatagcag gccgggggcc agcaggaggt acggcacggt gcggccgaag
     5701 cgggctcgca gcgtcgggcc ccgcggcgcg cttgcggttg cggcggtcat cgaccggtcg
     5761 tcatgtcggg gtggcttcgg ctcaggtccc ggacagcttc gagaagaggt cgttcagctt
     5821 cttctcgatg tcgtcgctga gcgactggaa gttgtgttgc ttggcgacga cctccggggt
     5881 cgggaagagg agcgggttcg ccgccgcctc ggcatcgagc ttcttgatct cctcgtcagc
     5941 gcccttgacc ggcgagacgt agtagacgta gttcgcgatc tgggcggcga tcttcggatc
     6001 gtagacgtag ttcatcatca gctcggcggt gtacttgttg gccgcgccct tcgggatgag
     6061 catgttgtcg gtccagatca gcgtgccttc ctccgggaag atgaacttgt cgtcggggcc
     6121 gccggacgag gcgaggtcgc cggaccagac gaacgccgcc cagaccttct tctggccgaa
     6181 gtcctggagg tactcgttgc cggtgaagcg gagaccgccg tcggcgagcg gctggatcga
     6241 atcggcagcc tgctggagct gatcggccgt cacggtcgcg ccgttggcgt cgaccccgag
     6301 cttcagcagg acgagcccga aggtgtcgcg ggcctcggcc aggaacgtca gcttctccgg
     6361 cgggatgttc cagaggtcgg cgatcttcgt cggctcggcg atgttgttgg ccttgagggt
     6421 cttcgcgttg aacccgacgc cggtcatccc ggattgccac gggtagtggt agtcgttggt
     6481 cgggtcccag gtctggttct tcagggcgtc gcggatgttg gcggtgcagt tcgggacgtt
     6541 cgtctggtcg atcgtctcgg cccacttgtt ggccacgatc ttggacgcca tccagtcggt
     6601 caggacgacg aggtcccagc cg
//
LOCUS       DATAIW010000046         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1441, whole genome shotgun sequence.
ACCESSION   DATAIW010000046 DATAIW010000000
VERSION     DATAIW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VHM48_01665"
     CDS             complement(<1..395)
                     /locus_tag="VHM48_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754137.1"
                     /translation="MTAPDPTADDAFQRAAEELWMPVYRFALALTNDLGEADDVAQEA
                     FVRLWRTRGRIDWSEPVVGWLLVAARRIAFDRFRRLARELRLRPAPAVPVGIDAATVE
                     RFVDLRGQLSALTAHERAAILLSATEGWST"
     gene            complement(466..>1581)
                     /locus_tag="VHM48_01670"
     CDS             complement(466..>1581)
                     /locus_tag="VHM48_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2754138.1"
                     /translation="RIFYNQARMSGAGIPQVALVMGSSTAGGAYVPAMSDETVIVKGT
                     GTIFLGGPPLVKAATGEEVSAEDLGGGDVHTSISGVADHLALDDEHALALGRSIVANL
                     NRRPPTPPWDRLAPEAPAVDPSGGPGTAGLYAAVSADPRRPVEVREIIARLVDGSRFH
                     DFKPRYGETLVTGFAHVDGWPVGIVANDGILFSASALKGAHFVELCAQRKIPLVFLQN
                     ITGFMVGREYEAGGIAKDGAKMVTAVACAEVPKFTILIGGSFGAGNYAMAGRAYQPRF
                     LWTWPNSRISVMGGTQAARVLSTVGGPFADEAARDAFEAPILETYEREGSPYFATARL
                     WDDGVIDPLDTRRVLSLGIKAAFHAPIPDTRFGVFRM"
BASE COUNT          233 a          567 c          572 g          209 t
ORIGIN      
        1 gtcgaccagc cctccgtcgc cgacagcagg atcgccgccc gttcgtgcgc ggtgagcgcc
       61 gacagctgcc cacggagatc gacgaaccgc tcgaccgtcg ccgcgtcgat cccgacgggg
      121 accgccggcg ccggtcgaag ccgaagctcg cgggccaggc gccggaagcg atcgaacgcg
      181 atgcgccgcg cggcgacgag cagccaaccc acgaccggct cgctccagtc gatcctccct
      241 cgtgtccgcc acagccggac gaacgcctcc tgggcgacgt cgtcggcctc tccgaggtcg
      301 ttggtcagcg cgagcgcgaa tcggtacacc ggcatccaca gctcctcggc ggcgcgttgg
      361 aaggcgtcgt cagcggtcgg gtcgggggcg gtcatctgtc ccgtacacgt ccaccgagcc
      421 gcgatcgcac ggccggcgcg cgccgattcg tcgcgggtgc ggggatcaca tccggaagac
      481 cccgaagcgc gtgtccggga tcggggcgtg gaaggcggcc ttgatcccga gcgagagcac
      541 ccgtcgcgtg tcgagcggat cgatcacgcc gtcgtcccac agccgggccg tggcgaagta
      601 cggcgagccc tcgcgctcgt acgtctcgag gatcggggcc tcgaacgcgt cgcgcgccgc
      661 ctcgtcggcg aacggcccac cgaccgtcga gaggacgcgg gccgcctggg tgccgcccat
      721 gacggagatg cgcgaattcg gccaggtcca caggaagcgc ggctggtagg cccggccggc
      781 catcgcgtag ttgcccgccc cgaagctccc gccgatgaga atcgtgaact tcgggacctc
      841 ggcgcaggcg accgcggtca ccatcttcgc cccgtccttg gcgattccgc ccgcctcgta
      901 ctcgcgcccg accatgaagc cggtgatgtt ctggaggaac acgagcggga tcttccgctg
      961 ggcgcacagc tcgacgaaat gggcgccctt gagggccgac gcgctgaaca ggatcccgtc
     1021 gttggcgacg atcccgaccg gccagccgtc gacgtgggcg aagccggtga cgagggtctc
     1081 gccgtagcgc ggcttgaagt cgtggaagcg gctgccgtcg acgagccggg cgatgatctc
     1141 gcggacctcg acgggccggc gggggtcggc cgagacggcg gcgtagagcc cggcagtccc
     1201 gggtccgccg gacgggtcga ccgccggggc ctccggcgcc agccgatccc agggcggcgt
     1261 cggcggccgc cggttcaggt tcgcaacgat cgaccggccg agggcgagcg cgtgctcgtc
     1321 gtcgagggcg aggtgatcgg ccacgccgga gatcgacgta tggacgtcgc cgccgccgag
     1381 gtcctcggcg ctgacctcct cgccggtcgc ggccttcacg agcggcggac cgccgaggaa
     1441 gatcgtgccg gtgcccttca cgatgacggt ctcgtcgctc atcgccggga cgtacgcacc
     1501 gccggcggtc gatgagccca tcaccaacgc gacctgcgga atgcccgcgc ccgacatccg
     1561 ggcctggttg tagaagatcc g
//
LOCUS       DATAIW010000047        14036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_179480, whole genome shotgun sequence.
ACCESSION   DATAIW010000047 DATAIW010000000
VERSION     DATAIW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179480"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VHM48_01675"
     CDS             <1..946
                     /locus_tag="VHM48_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754139.1"
                     /translation="ESGTRIVTRTGGLDLGPRVAAIPLEDYTRSMTEEGIAFDLLDGP
                     EISRRWPQWRLGDEHHGLYQADAGIADPNKGNAAHQRLARAHGATLLDHAPVASLRAL
                     AGGGIEVELADGTLHRAPHVVLAADAWTSDLLASFDRRLPLTIIQAQVTYFAAPDPAL
                     FAPDRFPIWIWMDDPSFYGFPTYGEAGPKAAEDVGGDEVTPATRTFERNEVGFRRLTD
                     FLARHLPEHLGPEIVTKTCLYTLTPDRDFVVDRLPDVPGVHVVLGAAHGFKFASVLGR
                     IVAELVVDGSTPSAGELERFAIDRPILLEANPATSFLL"
     gene            954..1922
                     /locus_tag="VHM48_01680"
     CDS             954..1922
                     /locus_tag="VHM48_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX2754140.1"
                     /translation="MSRASEDLAAAPRKPRRLREGDTVGVVAPASSWENRSELLRGIA
                     GLEAWGLNVKLGAHVNDRHGYMAGRDEDRVADIHAALADPEVRAIVCLQGGYGTPRLI
                     PLLDEAAFAANPKAICGYSDLTTLHLAQQHWGDVISFYSNGVTGIGAPEVTDFSKASL
                     KRALFSDEPYGQIGPNPDDPYVRTIVGGRVTGRLTGGCAGLLSSAIGTRLQPDFRGRI
                     VVLEEIDNEGYIHDGILTQLRNAGLFEGAAGIVVGDIKAKWSGGIAELSAEDIVEEVL
                     APLGLPLIFGLPIGHNKHHATVPLGALATLDADAGTLVVEEVVTTD"
     gene            2059..3978
                     /locus_tag="VHM48_01685"
     CDS             2059..3978
                     /locus_tag="VHM48_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2754141.1"
                     /translation="MRHPLRARLTRALVAVGLILSTILPGASASAAPGDLVLRAGTDQ
                     DLQILNPFESVLVADFEAFTLNYDLLVNFGPNIESVPGFAESWTQSTDGKTWTFKIRP
                     GMKWSDGQPATSEDARWTYQTILDGVASDRGYLGEGYLEGYLTDAGLKTVTAPDPTTL
                     VVTTDFANTLLLQAYVPILPKHIWSTHSLAQIIDPKAAKFFKNEPPVVGTGPYQAVEW
                     KPGDFIRFARNPNYWGKQGAVDEVIIQHFASSDTMVQALKTGEVDYVRGIQPDQFNAL
                     KTEPGITAVAGIANGYSELSFNTGGNKKGYHGSTSALSDIAFRDALGYAIDNQKLVEA
                     TLGGYGTPGSTIIPPFHTRWHVEPDRPRKFDLAEANTRLDAAGYTKNADGKRVDKDGK
                     VINLRLTWPDSEAEDATNAQFLVEWFGQLGITVTAAVTEEGKLIDDVTGPPGAANYDI
                     YMWGWVGDPDPTSLLKFFQTSAIGSSSDSYYSNPHYDDLFKQQRAESDPVKRKALLAE
                     MQNLVYDEAPYHILYYDAELDAYRTDKFAGWTNQPVEGGTPLFGYGSGNYTLLTDASA
                     TPSAAPSVAPSAAASTVPGASGGTPAASAPPDTTPASSSSSSLPLILGVIALVVIIAV
                     GLVLMRGRRREIDEE"
     gene            4103..5080
                     /locus_tag="VHM48_01690"
     CDS             4103..5080
                     /locus_tag="VHM48_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754142.1"
                     /translation="MKIGYLGRRIASALITIALIALINFLLFRAMPGSPERVILGRTP
                     NVTAQILQQTRERWGLDKSLFPDQFVAYVGATVQGDLGFSFIARGQTVTEVLAQRIWP
                     TVILFGLGEIVAIILGLALGAYTGWRRGGPVDYVGNGVSLVLYSTPYFLLGMAFLLVF
                     ATTFRWFPTFGMLTSGAQYPGLWERLIDFLAHLALPLATVAFGLVGQYAIVMRSSIVE
                     TLSEDYVTTARAMGLREGRILRHHALPNAMLPTVSLIAINLGYVVAGAITVEVVFNWP
                     GLGTLAVTALTSRDYPVLQGVFLLLGISVVLANLVADLIYGALDPRVRT"
     gene            5077..6021
                     /locus_tag="VHM48_01695"
     CDS             5077..6021
                     /locus_tag="VHM48_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754143.1"
                     /translation="MTAASVRGTGPRGLGRALGSGTIVRRLLRKPEGLIGVVILTIFI
                     VLAIVPQVFVGPLQTAVTASGHRLEPPSGAHLFGTDELRRDMLNQTVYGARISLVIGF
                     LATLVTVAIGAVIGIVAGFVGGWLDAILMRITDFFLVIPTFVLALILTPIIQELVGSA
                     ATEVFGIRMTLIVIVIVIGITSWSSTARIIRSQTLSLRERAFVDRARVIGAGSGHIMR
                     RHILPNVINLIVANAVLTFAGAVLTETTLSFVGLGDPFQPSWGQLLEAARAAGAPGLG
                     AWWYFVPPGAGIVLVVLSFTLVGSALDDLLNPKRGGRR"
     gene            6096..7178
                     /locus_tag="VHM48_01700"
     CDS             6096..7178
                     /locus_tag="VHM48_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754144.1"
                     /translation="MPKHADPSQPLLVVDDLRTYFELDGATVHAVDGVSFKLQQGEAL
                     GIAGESGCGKTTTALSLIRVLPSNARIVSGSVRYFGIDLVPKSAEALRRYRWREISIV
                     FQGAMNALNPVHRLRDQVAEPLMERLNMSESKARKRAGELLELVGIPKQRGSAYPHEL
                     SGGMRQRAMIAMALACDPAIVIGDEPTTALDVMIQAQILELLERLRRELGLSLILITH
                     DLSVIAETCDRILVMYAGRVAEEGSVSEVFRHPRHPYTQKLLSAFPNITADRRTLDVI
                     PGSPPDLREPPPGCRFAPRCPFVMPVCSEVVPPEVTFDGVRVACHLYPPGSDGAPAGM
                     LARSSTPEADGGAPSVSTRPAEAPRA"
     gene            7175..8368
                     /locus_tag="VHM48_01705"
     CDS             7175..8368
                     /locus_tag="VHM48_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754145.1"
                     /translation="MTDPIRPTIAGAGAGPVASGGAGAGAPPRAPAEELLRLTDLKVH
                     FPIRTGVADTILRRSHGAVRAVDGIDLTLHRGEVLGLVGESGSGKTTTGRVIVKLTRQ
                     TAGTMTFDGKDVSDLRGTKALRPYRRRVQLIFQDPYETLNPKQTINDFVMEPLVVNDL
                     GQSRDDRQRRVFGALEAAGLRPAADFAFRYPHELSGGQRQRVVIAGALVMGPELIVAD
                     EPVSMLDVSIRTELLRLMLDLRAERGLTYLFITHDLSLAWVIADRIAVMYLGKIMEIG
                     PAEAVIGSPHNPYTQALVSVAPSPDPPTEGRRARRTILVGETPDAAHVPSGCRFHPRC
                     PYAFDRCRVEEPPLFDVGAGHQAACWLAEGGRSLPVLPPHEVGEVVQPGIAAPTSPPP
                     SSTPA"
     gene            8368..8928
                     /locus_tag="VHM48_01710"
     CDS             8368..8928
                     /locus_tag="VHM48_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2754146.1"
                     /translation="MAFDPADVTSTEGLEPLTTAHVAAFLRAACDHIVTELTALGDDW
                     ARWRPAPEEWSANECLGHIIEADRRGFAGRIRRILALDGVAEQGWDQIATAAARRDND
                     RTVAAILEEFRTGRDAAIGLVDGLGPDDLERFAIHDRVGRVTVGELLHEWVFHDRNHI
                     RQLLANTQALAWPAMGNTRRFSHPDR"
     gene            complement(8952..9605)
                     /locus_tag="VHM48_01715"
     CDS             complement(8952..9605)
                     /locus_tag="VHM48_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX2754147.1"
                     /translation="MEARKYLAELVGTFILVVVGSMTIVGAGRTGAPILAVVPFGFGL
                     GLLAAIQSAGYVSGGHFNPAVTLGALLDRRIDVMNAVGYIVAQVIGAIAASAVVLVVA
                     SQAEVQSTASGFPAGKMVNAFAVELLLTAIFVLVILTVTKRAPSHAAFAIPLTLTVIH
                     FAGIPFSGASVNPARSLAPALVGGTLDGNIIVWIAGPLLGAVLGWAVYRALEDGSTR"
     gene            complement(9714..9851)
                     /locus_tag="VHM48_01720"
     CDS             complement(9714..9851)
                     /locus_tag="VHM48_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754148.1"
                     /translation="MIIIECTWCAAELALDSLDATSVDCADCRITVEIAPDPEPIAIA
                     A"
     gene            complement(9889..10518)
                     /locus_tag="VHM48_01725"
     CDS             complement(9889..10518)
                     /locus_tag="VHM48_01725"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX2754149.1"
                     /translation="MTDPGFDPTLARCSWSGAGSERPDPAMVRYHDEEWGAPCHDDTE
                     LFERLALESFQAGLSWSTILHKRDAFRQAFRGFDPAVVAGFTDADRERLMADAGIVRN
                     RAKIDATIGNAASWLATGREFGSVDAYLATMVPGPPQRVGPDGRAGEIPARTDASDAL
                     SADLRRRGFRFVGSTIVYALMQSIGLVDDHLPSCFRYGSPADTSGPARS"
     gene            complement(10563..11852)
                     /locus_tag="VHM48_01730"
     CDS             complement(10563..11852)
                     /locus_tag="VHM48_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX2754150.1"
                     /translation="MPGRSALVFAAIAALALSALGPALRIAAVSADGASGLPDLTFYG
                     RGYGHGVGMSQYGARGRALAGQLAPEILGHYYAKTTLGTRNAATAVRVLLLTGLAATP
                     ARPLKVVGLVGGWTIDGIAPTFPANARLVLSPTAPKATSWNLRVVSATGARLAAKTVS
                     GDVYVRPINALSVLQLVSKATTTNVYRGFFRLHLTTTAIVVNHIAVDRYLRSVVPLEM
                     PSTWPVEALKAQAIAARSYALYRVHRTTGLFDVYDDTRSQVYRGKRVETSATNTAVTA
                     TAGAVLLSGTSLVNALFHSADGGWTENNENVFVSSSGSIVAGAVSYLRGSSDRAPNGT
                     AYDKASPYATWMTGTYTAAALSTMFATDPRTAVGTLSDLDLSNRGVSGRLISVTLIGA
                     RGTKTVSGDVFRAVFNAARPATDPSLRGTLFDTVPIP"
     gene            12081..12626
                     /locus_tag="VHM48_01735"
     CDS             12081..12626
                     /locus_tag="VHM48_01735"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX2754151.1"
                     /translation="MAARFYVGHGASGSAASMAAHVRGLIARGIDARAIDLPLKKAEA
                     AVPALRAIVPDGPAAPGADGHAIAVGGQSYGGRVATLAAAEPDTDYAALVLFSFPLHP
                     PGAPARAEERTAHFPAIRCPALFLSGEADPFARLDLLRARVALLADAELVTYPRLGHT
                     LKPVLEDALDRAAAFLLARTT"
     gene            12913..13764
                     /locus_tag="VHM48_01740"
     CDS             12913..13764
                     /locus_tag="VHM48_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX2754152.1"
                     /translation="MAGRRRAVPVALLVAALLVSIVPAVVTASSPPGLERFLYALGQV
                     ESGGNYTARNTTSGAYGKYQIMPASWAAWAKTYLGSSTAPQTPTNQEIVAHRKVTALY
                     NWLDSWPTVAHWWLTGSSERNSSLWSSFSRTYVQRLMTIYYAAADVSGDVHTTSTTSW
                     ITSGDTRLSETSKAIAYHLAWSTATYASYSGRQVKYATRTGASATVAFSGTGIAWIGP
                     VGPTRGTARVYIDGKAVATVDLRRSTFQARKILFSRALAAGPHTLKIVVTSSGRPVAI
                     DNLIVGT"
BASE COUNT         2103 a         5059 c         4748 g         2126 t
ORIGIN      
        1 cgaaagcgga acgcggatcg tgacgaggac cggtggcctc gacctcggcc cgcgtgtcgc
       61 cgccatcccg ctcgaggact acacccgctc catgacggag gagggcatcg ccttcgatct
      121 cctcgacggt ccggagatca gccggcgctg gccgcagtgg cggctcggcg acgagcatca
      181 cggcctgtac caggccgacg ccgggatcgc cgacccgaac aagggcaacg cggcccacca
      241 gcgcctcgcc cgcgcccacg gggcgacgct gctcgaccac gccccggtgg ccagcctccg
      301 cgccctcgcc ggcggcggga tcgaggtcga gctcgcggac ggcacgctcc accgggcgcc
      361 gcacgtcgtc ctcgccgccg acgcctggac gagcgacctc ctcgcgtcct tcgaccggcg
      421 cctgccgctg acgatcatcc aggcccaggt cacctacttc gccgcgccgg atcccgccct
      481 gttcgccccc gaccgcttcc cgatctggat ctggatggac gacccctcgt tctacggctt
      541 cccgacgtat ggcgaggccg ggccgaaggc cgccgaggat gtcggcggcg acgaggtgac
      601 ccccgcgacc cggacgttcg agcggaacga ggtcgggttc cgccgcctga ccgacttcct
      661 ggcgcgccac ctcccggagc atctcgggcc ggagatcgtc acgaagacgt gtctctacac
      721 cttgacgccg gatcgggact tcgtcgtcga ccggctgccg gacgtgcccg gagtccacgt
      781 cgtgctcggg gctgcccacg gcttcaagtt cgcctcagtc ctcggtcgga tcgtcgcgga
      841 gctcgtcgtc gacggctcga ccccgtcggc gggtgagctc gagcgcttcg cgatcgaccg
      901 gccgatcctg ctcgaggcga accccgcgac gagcttcctc ctatgaccag tcgatgagcc
      961 gcgcatccga ggatctcgcc gccgcgccgc gcaagccgcg ccgcctccgc gagggcgaca
     1021 cggtcggtgt cgtcgcaccc gcgagctcct gggagaaccg cagcgagctc ctccgcggca
     1081 tcgccggtct cgaggcatgg ggcttgaatg tcaagctcgg cgcccacgtc aacgaccgcc
     1141 acggctacat ggccggtcgg gacgaggacc gggtggcgga catccacgcg gccctcgccg
     1201 accccgaggt ccgggccatc gtctgcctcc agggcggcta cggcacgccg cgcctgatcc
     1261 cgctcctcga cgaggccgcg ttcgccgcca accccaaggc catctgcggc tacagcgacc
     1321 tgaccaccct ccacctcgcc cagcagcact ggggcgacgt gatcagcttc tactcgaacg
     1381 gcgtgacggg gatcggtgcg cccgaagtca cggacttctc gaaggcgagc ctcaagcgcg
     1441 ccctgttcag cgacgagccc tacggccaga tcgggccgaa cccggacgat ccatacgtcc
     1501 ggacgatcgt cggtggccgg gtgacggggc ggctgaccgg cgggtgcgcc gggctcctga
     1561 gcagcgccat cgggacaagg ctccagccgg acttccgcgg tcggatcgtg gtgctcgagg
     1621 agatcgacaa cgagggctac atccacgacg ggatcctgac ccagctccgg aacgccgggc
     1681 tgttcgaggg tgccgcgggg atcgtcgtcg gcgatatcaa ggcgaaatgg tccggcggga
     1741 tcgccgagct gtcggccgag gacatcgtcg aggaggtcct ggccccgctc gggctgccgc
     1801 tgatcttcgg cctgccgatc ggccacaaca agcaccacgc caccgtcccc ctcggcgccc
     1861 tcgccacgct ggacgcggat gccggcacgc tcgtcgtcga ggaggtggtc accaccgact
     1921 gatcgcggac cacgatgcga gtcggagttg ccgtggtccg cagtgctgtg cgacagttcg
     1981 gccgcctctc gggaccgggg tccggaggcg atcatgtccg tcgcccgtcg tggcgaaccg
     2041 gacagacgag gaggagagat gcgacatccc ctgcgcgcgc ggttgacccg ggctctggtg
     2101 gccgtggggc tgatcctctc gacgatcctg ccgggagcct ccgcgtccgc cgctccaggc
     2161 gatctcgtcc tgcgagccgg caccgaccag gacctccaga tcctcaaccc gttcgagtcg
     2221 gtcctcgtcg ccgacttcga ggcattcacc ctcaactacg acctgctcgt gaacttcggg
     2281 ccgaacatcg aatcggtgcc gggtttcgcc gaatcctgga cgcaatcgac ggacggcaag
     2341 acctggacct tcaagatccg cccaggcatg aagtggtccg acgggcaacc cgcgacctcc
     2401 gaggacgccc gctggaccta ccagaccatc ctcgacggcg tcgcctccga ccgcggctat
     2461 ctcggcgagg gctacctcga gggctatctg accgacgccg gcctcaagac ggtcaccgca
     2521 ccggatccga cgacccttgt cgtgacgacc gacttcgcca acaccctgct cctccaagcc
     2581 tatgtcccga tcctgccgaa gcacatctgg tcgacccact cgctcgccca gatcattgat
     2641 ccgaaggcgg ccaagttctt caagaacgag ccgccggtgg tcggcacggg gccgtaccag
     2701 gccgtcgaat ggaagcccgg cgacttcatc cggttcgccc gcaacccgaa ctactggggg
     2761 aagcagggcg ccgtcgacga ggtgatcatc cagcacttcg cgagctcgga cacgatggtc
     2821 caggcactca agaccggcga ggtcgattac gtccggggca tccagccgga ccagttcaac
     2881 gccctcaaga ccgagccggg catcacggcg gtcgcgggca tcgccaacgg ctattccgag
     2941 ctgtcgttca acaccggcgg caacaagaag ggctaccacg gctcgacctc cgccctgtcg
     3001 gacatcgcct tccgcgacgc cctcggctac gcgatcgata accagaagct cgtcgaagcg
     3061 acgctcggcg gctacggcac gccgggctcg acgatcatcc cgcccttcca tacccgctgg
     3121 cacgtcgagc cggatcgacc gcgcaagttc gacctcgcgg aggcgaatac gcggctcgac
     3181 gcggccggct acacaaagaa tgcggacggc aagcgcgtcg acaaggatgg caaggtcatc
     3241 aacctccgcc tgacctggcc agactccgag gccgaggacg cgaccaacgc ccagttcctc
     3301 gtcgagtggt tcggccagct cgggatcacg gtgacggccg cggtcaccga ggaaggcaag
     3361 ctgatcgacg acgtgaccgg accgcccggt gcggccaact acgacatcta catgtggggc
     3421 tgggtcggcg acccggatcc gacgtcgctg ctcaagttct tccagacgag cgcaatcggc
     3481 agctcgagcg acagctacta ctcgaacccg cactatgacg acctgttcaa gcagcagcga
     3541 gccgagtcgg atccggtcaa gcgcaaggcc ctccttgccg agatgcagaa cctcgtctac
     3601 gacgaggcgc catatcacat cctctactac gacgccgagc tcgacgcgta ccggaccgac
     3661 aagttcgcgg gctggacgaa ccaaccggtt gagggtggga cgcccctgtt cggttatggc
     3721 tcgggcaact acaccctcct gacggatgcg tccgcgacgc cgtcggcggc gccatcggtc
     3781 gcgccgtcgg ccgcggcttc gacggtgccc ggggcaagcg gcgggacccc tgccgcgtcg
     3841 gctccgcccg acacgacgcc ggcgtcgagc tccagctcgt cgctgccact gattctcggt
     3901 gtgatcgccc tcgtggtcat catcgccgtc ggtctcgtcc tcatgcgcgg ccggcgccgg
     3961 gagatcgacg aggaatgagg gtcgcgggcg gcatgccgcc cgttgatcgg ccgccacgga
     4021 cggggagcgc cagccggcac tccccgtccc acgccgcggc cccgctgcgg tcatcacgtc
     4081 ccggcgtgtc cggccgccgc cggtgaagat cggctatctc ggtcgccgca tcgcctcggc
     4141 gctcatcacg atcgccctga tcgccctcat caacttcctc ctcttccggg cgatgcccgg
     4201 ttcgcccgag cgcgtgatcc tcggtcggac gccgaacgtg accgcgcaga tcctgcagca
     4261 gacgcgcgaa cgctggggcc tcgacaagtc gctgttcccc gaccagttcg tggcctacgt
     4321 cggggcgacc gtgcagggcg acctcggctt ctccttcatc gcccgtggcc agaccgtgac
     4381 cgaggtcctc gcccagcgga tctggccgac cgtcatcctc ttcggtctcg gcgagatcgt
     4441 cgcgatcatc ctcggactgg cgctcggcgc gtacacgggc tggaggcgcg gcggcccggt
     4501 cgactacgtc gggaacgggg tctcgctcgt cctgtattcg acgccgtact tcctgctcgg
     4561 gatggcgttc ctcctcgtgt tcgcgacgac gttccgctgg ttcccgacgt tcggcatgct
     4621 gacctccggc gcgcagtacc cgggcctgtg ggagcgcctg atcgacttcc ttgcccatct
     4681 cgctctgccg ctggcgacgg tggcgttcgg gttggtcggc cagtacgcga tcgtcatgcg
     4741 ctcctcgatc gtcgagaccc tgtccgagga ctacgtgacg accgcccggg ccatgggact
     4801 ccgtgagggc cggatcctgc gccaccatgc ccttccgaac gccatgctgc cgaccgtgtc
     4861 gctcatcgcg atcaatctcg gctatgtcgt cgcgggggcg atcaccgtcg aggtcgtgtt
     4921 caactggccg ggcctcggga cgttggccgt gacggcgttg acgtcgcggg actacccggt
     4981 cctccagggc gtgttcctgc tgctcggcat ctccgtcgtg ttggccaacc tggtggcgga
     5041 cctcatctac ggcgcgctcg acccgcgggt ccggacatga ccgcggcctc cgtccggggc
     5101 acggggccgc gcggcctggg ccgtgcgctc ggctccggga cgatcgtccg gcggctgctc
     5161 cggaagccgg agggcctcat cggcgtcgtg atcctcacga tcttcatcgt cctcgccatc
     5221 gtgccgcagg tgttcgtcgg gcccctccag acggcggtga ccgcgtccgg ccatcggctc
     5281 gaaccgccgt ccggggccca cctgttcggc accgatgagc tgcgccgcga catgctgaac
     5341 cagaccgtgt acggcgcccg catctcgctc gtcatcgggt tcctcgcgac cctcgtgacc
     5401 gtcgcgatcg gggccgtgat cgggatcgtc gccgggttcg tcggcggctg gctggacgcg
     5461 atcctcatgc ggatcacgga cttcttcctc gtcatcccga cgttcgtgct cgccctgatc
     5521 ctgacgccga tcatccagga gctcgtcggg tcggcggcga ccgaggtgtt cgggatccgg
     5581 atgaccctga tcgtcatcgt gatcgtcatc gggatcacga gctggtcgtc gaccgcccgg
     5641 atcatccgct cccagacact gtcgctgcgg gagcgggcct tcgtcgaccg tgctcgcgtg
     5701 atcggggctg gtagcggcca catcatgcgc cgccacatcc tgccgaacgt catcaacctc
     5761 atcgtcgcca acgcggtcct gaccttcgcc ggagcggtcc tgaccgagac cacgctttcg
     5821 ttcgtcggcc tgggcgaccc gttccagccg tcctggggcc agctgctcga ggcggcccgg
     5881 gccgccggag cgccgggcct cggtgcctgg tggtacttcg tgccgcccgg cgccgggatc
     5941 gtcctggtcg tgctgtcgtt cacgctcgtc ggcagcgcgc tcgacgatct cctcaacccg
     6001 aagcggggcg gtcggcgatg atcggacggg gaggcgccgg gccaaagtca tcgacggcga
     6061 ccgccgcgac gccggtcgat gcccgcggac gtgcattgcc gaagcacgca gatccgagcc
     6121 agcccctgct cgtcgtcgac gatctccgga cgtacttcga gctcgacggc gcgacggtcc
     6181 atgcggtcga tggcgtcagc ttcaagctcc agcaaggcga agcgctcggg atcgccggcg
     6241 agtccggctg cggcaagacg acgacggcgc tgtcactgat ccgtgtgctg ccgtccaacg
     6301 cccggatcgt gagtggctcg gttcgctact tcgggatcga tctcgtgccg aagagcgccg
     6361 aagccctgcg gcgctaccgc tggcgcgaga tctcgatcgt gttccagggg gcgatgaacg
     6421 cgctcaaccc ggtccaccgg ctgcgcgacc aggtcgccga gccgctgatg gagcggctga
     6481 acatgtccga gtcgaaggcg cgcaagcggg ccggcgagct cctcgagctc gtcgggatcc
     6541 cgaagcagcg ggggagcgcc tatccacacg agctgtcggg cgggatgcgc cagcgggcaa
     6601 tgatcgcgat ggccctcgcc tgtgatccgg cgatcgtcat cggcgacgaa ccgacgactg
     6661 cgctcgacgt gatgatccag gcccagatcc tcgagctgct cgagcggctt cgccgggagc
     6721 tcgggctgtc gctcatcctg atcacccacg acctgtcggt tatcgccgag acctgcgacc
     6781 ggatcctcgt gatgtatgcc ggtcgggtcg ccgaggaagg ctcggtcagc gaggtcttcc
     6841 gccatccccg ccatccctac acgcagaagc tcctgtcggc attcccgaac atcacggccg
     6901 atcggcgaac gctcgacgtg atcccggggt ctccgccgga cctgcgcgaa ccgccgccgg
     6961 gctgccgctt cgcaccacgc tgcccgttcg tcatgccggt ctgcagcgaa gtcgtcccac
     7021 ccgaggtcac cttcgacggg gtgcgagtcg cctgccacct ctatccgccg gggagcgacg
     7081 gggcacccgc cggcatgctc gcccggagct cgacgccgga agccgatggc ggggcgcctt
     7141 cggtgtcgac gaggccggcc gaggcaccgc gcgcatgacc gacccgatcc gcccaacgat
     7201 cgcaggcgca ggcgcaggcc cagtcgcgtc gggcggagcc ggcgccggcg cgccaccgag
     7261 agcgcccgcc gaggagctgc tgcgcctgac cgacctcaag gtccattttc cgatccggac
     7321 cggcgtcgcg gacacgatcc tgcggcggtc gcacggtgcc gtccgggcgg tcgatgggat
     7381 cgacctgacg ctgcaccggg gcgaggtcct cggcctcgtc ggggagtcgg ggagcggcaa
     7441 gacgacgacc ggccgggtca tcgtcaagct gacccgccag accgccggca cgatgacgtt
     7501 cgacggcaag gacgtcagcg acctgcgcgg caccaaggct ctgcgcccgt atcgccgccg
     7561 ggtccagctc atcttccagg atccgtacga gacgctgaac ccgaagcaga cgatcaatga
     7621 cttcgtgatg gagccgctcg tcgtcaacga tctcgggcag tcacgggacg atcgccagcg
     7681 ccgggtgttc ggcgcgctcg aggcggcggg tctccggccg gcggccgatt tcgcgttccg
     7741 atacccccat gagctctcgg gtggccagcg ccagcgggtc gtcatcgccg gcgccctcgt
     7801 catgggcccc gagctgatcg tcgccgacga gccggtctcg atgctcgacg tgtcgatccg
     7861 gaccgagctc ctccggctga tgctcgacct tcgcgccgag cgcggcctga cctacctctt
     7921 catcacccac gacctgtcgc tggcgtgggt gatcgccgac cggatcgcgg tgatgtacct
     7981 cggcaagatc atggagatcg gtccggccga ggcggtcatc ggctctccgc acaatcccta
     8041 cacgcaggcg ctcgtgtccg tcgcgccatc cccggatccg ccgacggagg ggcgacgagc
     8101 gaggcggacg atcctcgtcg gcgagacgcc cgacgcggcc cacgtcccaa gcgggtgccg
     8161 gttccacccg cgctgcccgt acgcgttcga tcgctgccgg gtcgaggagc cgccgctgtt
     8221 cgatgtcggc gccgggcacc aggcggcctg ctggctggcg gagggcgggc gctccctgcc
     8281 cgtcctgccc ccgcacgagg tcggtgaggt cgtccagccc gggatcgccg cgccgacctc
     8341 tccgcccccg tcatcgaccc cggcctgatg gcgttcgatc ccgccgacgt cacgtcgacc
     8401 gagggtctcg agccgttgac gacggcgcac gtcgccgcgt tcctgcgcgc cgcctgcgac
     8461 cacatcgtga ccgagctgac cgcgctcggc gacgactggg cgcgctggcg cccggcgcca
     8521 gaggagtggt cggccaacga gtgtctcggc cacatcatcg aggcggatcg acgcgggttt
     8581 gccggacgga tccgacggat cctcgctctt gacggcgtcg ccgagcaggg ctgggatcag
     8641 atcgcgaccg ccgcggcgcg acgcgacaat gaccggaccg tcgccgcgat cctcgaggag
     8701 ttccgcaccg ggcgcgacgc ggcgatcggc ctcgtcgacg ggctcgggcc ggacgacctc
     8761 gagcggttcg ccatccacga tcgggtcgga cgggtgaccg tcggcgagct cctccatgaa
     8821 tgggtcttcc acgatcgcaa tcacatccgc cagctgctgg caaacacgca ggcgctggcg
     8881 tggccggcga tgggcaacac ccgccggttc agtcaccccg accgctgacg gacgcccggc
     8941 gagctaccgc gtcagcgcgt ggagccgtct tcgagggcgc ggtagacggc ccagccgagc
     9001 accgccccga gcagcggacc ggcgatccag acgatgatgt tcccgtcgag cgtgccgccg
     9061 acgagtgccg gagcgaggga ccgggccggg ttcaccgagg cgccgctgaa cgggatgccc
     9121 gcgaagtgga tgacggtcag cgtcagcggg atcgcgaagg cggcatggct cggggcacgt
     9181 ttggtgaccg tcaggatcac gagcacgaag atcgcggtca gcagcagttc cacggcgaac
     9241 gcattgacca tcttgcctgc ggggaagccg ctcgcggttg actgcacctc ggcctgactc
     9301 gccaccacga gcacgaccgc cgatgcagcg atcgcaccga tcacctgggc gacgatgtag
     9361 ccgaccgcgt tcatcacgtc gatccgccgg tcgagcaggg ccccgagcgt gaccgcgggg
     9421 ttgaagtggc cgcccgacac gtagccggcc gactggattg ccgcgagcag cccgagcccg
     9481 aatccgaacg gcacgaccgc gagaatcggg gcgcccgtcc tgccggcgcc gacgatcgtc
     9541 atcgatccga cgacaaccag aatgaacgtc ccgaccagct cggcgaggta cttgcgagct
     9601 tccatcccat cctccgttgc acgagtgccc gtcacgcgcc aggacgcagt atggcggctc
     9661 gggtcaggcc ggcgagacgt gcccgcgcag gatgggaccg gcgccgcgtc gggtcaggcg
     9721 gcgatggcga tcggctccgg gtccggggcg atctcgacgg tgatccggca atccgcgcaa
     9781 tcgacgctcg tcgcgtcgag gctgtcgagg gcaagttccg cggcacacca ggtgcattcg
     9841 atgatgatca tggccggagc ctgcgccccg gtcgtgacag ctagcgtgtc acgaccgcgc
     9901 gggtcctgac gtgtcggccg gtgatccgta tcggaagcag ctcggcaggt gatcgtcgac
     9961 gaggccgatg ctctgcatca atgcgtagac gatcgtcgag ccgacgaacc ggaagccgcg
    10021 tcggcgcagg tcggccgaca acgcgtccga ggcgtccgtt cgagccggga tctcgccggc
    10081 gcgtccgtcg gggccgaccc gctgcggtgg gcccggcacc atcgtcgcca gatacgcgtc
    10141 gaccgaaccg aactcgcggc cggtggccag ccacgatgcg gcattcccga tggtcgcatc
    10201 gatcttcgcc cggttccgga cgatcccggc atccgccatc aggcgctccc gatccgcgtc
    10261 ggtgaacccg gccacgaccg ccggatcgaa gccgcggaat gcctgacgga acgcatcccg
    10321 cttgtggagg atcgtcgacc agctgaggcc ggcctggaac gactcgaggg cgagccgctc
    10381 gaagagctcc gtgtcgtcgt ggcacggcgc cccccattcc tcgtcgtggt agcggaccat
    10441 cgccggatcc ggccgctcgc ttccggcccc ggaccaggag cagcgggcaa gggtcggatc
    10501 gaatccggga tcggtcacag cccgacgata cctggccacg ccggctcatg ccggtcggcg
    10561 gttcagggga tcgggacagt gtcgaagagc gttccgcgga gggacggatc ggtggcgggc
    10621 cgggcggcgt tgaagaccgc ccggaacacg tcccccgaca cggtcttcgt cccgcgagcg
    10681 ccgatcagcg tcacgctgat cagccggccg gagacgccgc ggttcgacag gtcgagatcg
    10741 ctcagtgtcc cgacggccgt tcgggggtcc gtggcgaaca tcgtgctcag cgccgcggcc
    10801 gtgtaggtgc ccgtcatcca cgtcgcgtac ggcgacgcct tgtcgtacgc ggtcccgttc
    10861 ggggcgcggt cgctcgatcc ccgcaggtac gagaccgcgc ccgccacgat cgacccgctc
    10921 gagctgacga agacgttctc gttgttctcg gtccagccgc cgtcggcgga gtggaacagc
    10981 gcgttcacca gggacgtgcc gctcagcagc accgcgcccg cggtcgccgt cacggccgtg
    11041 ttggttgctg acgtctcgac gcgcttgcct cgatagacct gcgaacgcgt gtcgtcgtac
    11101 acgtcgaaca ggccggtcgt ccggtggacc cggtagaggg catatgaccg ggcggcgatc
    11161 gcctgggcct tcagcgcctc gaccggccag gtcgacggca tctcgagcgg aacgacgctg
    11221 cgcaggtagc ggtcgaccgc gatgtggttg acgacgatgg ccgtcgtcgt caggtggagg
    11281 cggaagaagc cgcgatagac gttggtcgtc gtcgctttgg agaccagctg gagcacgctc
    11341 aacgcgttga tcgggcggac atagacatcg ccgctcacgg tcttcgcggc cagccgagcc
    11401 ccggtcgccg acacgacccg caggttccag ctcgtggcct tcggcgcggt cggggagagg
    11461 acgagcctgg cgttcgccgg gaaggtcggc gcgatgccgt cgatcgtcca gcccccgacc
    11521 agccccacga ccttcagcgg tcgggccggt gtcgcggcga gcccggtcag cagcagcacc
    11581 cggaccgccg tcgccgcgtt acgtgtcccg agggtggtct tcgcgtagta gtggccgagg
    11641 atctccggcg ccagctggcc ggccagcgcc cggccgcgag ccccgtactg cgacatcccg
    11701 accccgtgac cgtaaccgcg accgtagaac gtcaggtccg gcaggccgga cgcgccgtcg
    11761 gcggacacgg ccgcgatccg gagggcgggt ccgagcgccg acagggcgag agcggcgatg
    11821 gcggcgaaga cgagcgccga ccgtccgggc aacgcacgcc gcagcggccg gggcgggaag
    11881 ccggcggaac gggagcggcg gatcgagagc gtaggcagca tcgattcctc tgcggacccg
    11941 accaccatga acggtgcgga cgcgcaccgg ccatggcccg acggggccag cgatcccatc
    12001 cgatcgggtc aggccgcgct gagcgcggcc ccggtcgatc gcgagcgcag tcccgggcgt
    12061 tcgcggtcta ccctgtctcg atggcggccc gattctacgt cggtcacggc gcgagcggct
    12121 cggcggcgag catggccgcc catgtccgcg ggttgatcgc ccgcgggatc gacgcccgcg
    12181 ccatcgacct gccgttgaag aaggccgagg ctgccgtccc ggccttgcgg gcgatcgtcc
    12241 ccgacggacc ggccgcgccc ggggcggacg gccacgcgat cgccgtcggc ggccagtcgt
    12301 acggcggccg ggtcgcgacc ctggcagcgg ccgaaccgga caccgactat gccgcgctcg
    12361 tcctgttcag ctttccgctc catccgcccg gcgcaccggc cagggccgag gagcgaacgg
    12421 cccatttccc ggcgatccgg tgtccggccc tgttcctgtc cggtgaggcg gatccgttcg
    12481 cccggctcga cctgcttcga gcacgcgtcg ccctcctggc cgatgccgag ctcgtcacgt
    12541 atccgcggct cggccatacg ttgaagccgg ttctcgagga cgcgcttgat cgggcggcgg
    12601 cgttcctgct cgctcggacg acgtgacgcg gcgagccgac tgctgacacc cccgggcggg
    12661 accgatcccg atttgccacg ggcttgcaac gccggatact ggacaacgcc cgtatgctgg
    12721 gccgtacccc aggggctgtg gcgggtcggg aggacgcgca gccaaatcga agacgttgcc
    12781 gacggaagct ccccccgcgg cgtacgcgct gaaaccccaa ggcgcctgcg tcctttccgc
    12841 ccggctctcg tccgtccatc gctcggcgcg ctcaccgcgc cgtcccggta ggaaggagca
    12901 ccattgccag acgtggctgg ccgccgtcgc gcggtccccg tggccctgtt ggtcgcggca
    12961 ctcctcgtct cgatcgttcc ggcggtcgtg acggcatcgt cgccaccggg cctcgagcgg
    13021 ttcctgtacg cgctcggcca ggtggaatcc ggcggcaact acaccgcccg caacacgacc
    13081 agtggcgcct acggcaagta ccagatcatg ccggcgagct gggcagcatg ggcgaagacg
    13141 tacctcggca gctcgacggc gccccagacg ccgacgaacc aggagatcgt ggcgcaccgc
    13201 aaggtgacgg ccctctacaa ctggctcgac tcgtggccga ccgtcgccca ttggtggctg
    13261 accgggtcgt ctgaacggaa ctcgagcctc tggtcgtcgt tctctcggac ctatgtccag
    13321 cgcctgatga cgatctacta cgccgccgcc gacgtgtccg gcgacgtgca caccacctcg
    13381 acgacgtcgt ggatcacatc gggcgacacc cggctcagcg agacatccaa ggcgatcgcc
    13441 taccacctcg cctggtcgac cgcgacgtac gcgtcgtatt cgggccgcca ggtgaagtac
    13501 gccacccgga ccggggcgtc ggcgaccgtg gccttcagcg gcaccgggat cgcctggatc
    13561 gggccggtcg gtccgacccg cggcacggcc cgcgtctaca tcgacgggaa ggcggttgcc
    13621 acggtcgacc tccgccggtc gacgttccag gcccgcaaga tcctgttctc gagggcgctt
    13681 gctgccgggc cgcataccct gaagatcgtc gtcacgagca gcggccgacc ggtcgcgatc
    13741 gataacctga tcgtcggaac ctgacctgac ctgacctgac ctgacccgac ctgccccgac
    13801 ctcggccctg agcccccagg cctggcgctc gacgcctgtt ccggttcgcc gggacgggcg
    13861 tcggcgttcg gaggccgttg gcgccgtcaa cggtccggtc ccggccggcc cgatgcaggt
    13921 tcctggccgg gcagtcgccg gaggactcaa atggaggggt ttcggctccg gaacggccgc
    13981 gtgagggctt caccgagcgt ggatcacacc tcgtgacccg tggaatcggc cgaatc
//
LOCUS       DATAIW010000048         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_61289, whole genome shotgun sequence.
ACCESSION   DATAIW010000048 DATAIW010000000
VERSION     DATAIW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61289"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2929)
                     /locus_tag="VHM48_01745"
     CDS             complement(152..2929)
                     /locus_tag="VHM48_01745"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX2754153.1"
                     /translation="MTDTTTTEPTDKMASPPPGGLPKAYRPSEVEGAIYERWLAADVF
                     APDGAGSRPDPDAEPFTIIQPPPNITGSLHLGHAQRAAVEDLMTRHARMTGKAALFLP
                     GLDHASIAAQFVLDGIIARDGESRQSLGRERYLERMQDFVTTTREVIVSQERRLGASL
                     DWNRLRYTMDDVSAKAVRVAFKTLHERDLAYRTEALVNWCPGCRTSVSDLEVVSTPET
                     GTLWSIRYHRVDDATGEPLPDESVTVATTRPETILGDTAVAVHPDDARYRALVGRRVR
                     IPFVARDVPIIADAVVDPAFGTGAVKITPAHDHDDHETGKRHGLAMPTILDDAAVVSG
                     TGTAYDGLDRYVARTAILADLDARGDLVGEKPHEMVLGRCQRSNDVIEPRLKTQWFVR
                     TEPLAAKALEATRSGRTRIVPDHFEKTWEHWLTTIRDWNVSRQLWWGHRIPAWYCPDG
                     HVTVSAELEGPSACEDCGRPAAELRQDPDIFDTWFSSGLWPFSTLGWPDETDDFRRFY
                     PTSVMETGYDIIFFWVARMMMLGIELTGSEPFHTITLSGLIRDPFGKKMSKTTGNVVD
                     PLAVMEEAGADALRFALIHGATSGQDQRFGPQKLELARNFANKLWNAARFVLGARPTE
                     IAVDAARTAPAEADLGPIERWIRSRAAAATAAVDRAMADYQFGEVTRALYDGIWSEFC
                     DWGIELAKVRLADESVPVDQRAATWWTLVDALDTYLRLLHPVMPFVTEAIWAAIPHRA
                     DDPDLLMVARWPAAAGRDERVDAALGEVIETIVAIRNARAHADLPAGAWLETHLAMAT
                     DSRATFEAMAPAIGRLARARPLVPHDRSADLPRPDGALEIVLPSGSFEATVLPPAASD
                     DAVDGAAGRDRARLEKELEEAEGHLVAARARLANTAFTDKAPPAVVEGARTRATELAE
                     QVARLRERLGR"
     gene            3335..3583
                     /locus_tag="VHM48_01750"
     CDS             3335..3583
                     /locus_tag="VHM48_01750"
                     /inference="COORDINATES: protein motif:HMM:NF033706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO4226 family nickel-binding protein"
                     /protein_id="HEX2754154.1"
                     /translation="MATFMDIHNGFVGVSRQQLVEAHERDLANEQAEGVHFEKAWLDP
                     ISGKVFCLSSGPSRESVMRVHERAGHPTAQVYEVPIEV"
     gene            complement(3643..>4080)
                     /locus_tag="VHM48_01755"
     CDS             complement(3643..>4080)
                     /locus_tag="VHM48_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2754155.1"
                     /translation="ADLLGAGSEIVAMDRDAGALARLARELPLRFPGCVLRTRVADFR
                     APLPAGRFDGVLAANSLHFVEDPIDVLRRARAELRPGGRLIVVEYDADRGNPWVPHPF
                     SARTFARLADAAGFASVREVGRVPSRFLGAIYAAAADRPDTEA"
BASE COUNT          562 a         1445 c         1503 g          570 t
ORIGIN      
        1 gcccgggcgt cgtgcggctc atggccgatc gatcacgtcc tcgtccgcgg gctcacggtc
       61 gacgcgtgcc gggtggcgac cgaggccggc gacgcctccg accactggcc ggtcgtcgtc
      121 gacctcagcc gcgcctgacg cccgccccag gtcagcggcc gaggcgctcc cgcagtcggg
      181 cgacctgctc ggcaagctcg gtggcgcggg tccgggcacc ctcgacgacg gccggcggcg
      241 ccttgtccgt gaaggcggtg ttggcgagcc gggcccgggc ggcgacgagg tggccctcgg
      301 cctcctcgag ctccttctcg aggcgagcgc gatcgcggcc ggcggcgcca tcgacggcgt
      361 cgtcgctcgc ggccggcggc aggaccgtgg cctcgaacga gccggacggc aggacgatct
      421 ccagcgcccc gtcgggccgc ggcaggtccg cggaccggtc gtgcgggacc agcggccggg
      481 cccgggccag gcgcccgatc gcgggagcca tcgcctcgaa cgtcgcccgc gaatcggtcg
      541 ccatcgcgag atgcgtctcc agccacgcgc cggcaggaag gtccgcgtgg gcccgggcgt
      601 tgcggatcgc cacgatcgtc tcgatcacct cgcccagcgc ggcatcgacc cgctcgtcgc
      661 ggccggcggc ggccggccag cgggccacca tcaacaggtc cggatcgtcg gcgcggtgcg
      721 ggatcgcggc ccagatcgcc tcggtcacga acggcatcac cgggtggagc aggcggaggt
      781 aggtgtccag ggcgtcgacc agcgtccacc acgtcgccgc ccgctggtcg accgggacgg
      841 actcgtcggc caggcggacc ttggcgagct cgatccccca gtcgcagaac tccgaccaga
      901 tgccgtcgta cagggcgcgg gtgacttcgc cgaactggta gtcggccatg gcgcggtcga
      961 ccgccgcagt cgccgccgcc gcgcgcgagc ggatccagcg ctcgatcggg ccgaggtcgg
     1021 cctcggcagg cgcggtccgg gcggcgtcca cggcaatctc cgtcggccgc gcgccgagca
     1081 cgaagcgggc cgcgttccag agcttgttcg cgaagttccg ggccagctcg agcttctgcg
     1141 gcccgaagcg ctggtcctgg ccggaggtcg cgccgtggat cagggcgaag cggagggcat
     1201 cggcacccgc ctcctccatc accgccagcg gatcgacgac gttgccggtc gtcttcgaca
     1261 tcttcttgcc gaacgggtcg cggatcaggc ccgacagggt gatcgtgtgg aacggctcgg
     1321 acccggtcag ctcaatcccg agcatcatca tccgggcgac ccagaagaag atgatgtcgt
     1381 agccggtctc catgaccgag gtcgggtaga agcgccggaa gtcgtcggtt tcgtcgggcc
     1441 agccgagggt cgagaacggc cacaaacccg agctgaacca ggtatcgaag atgtccgggt
     1501 cctggcggag ctcggcggcg ggtcgtccgc agtcctcgca ggcggacgga ccttcgagct
     1561 ccgccgagac cgtgacgtgg ccgtctgggc aataccaggc cgggatccga tggccccacc
     1621 acagctggcg cgagacgttc cagtcgcgga tcgtcgtcag ccagtgctcc caggtcttct
     1681 cgaagtggtc cgggacgatg cgggtccggc cgctcctggt ggcctcgagg gccttcgccg
     1741 cgagcggctc ggtccggacg aaccactggg tcttgagccg cggctcgatc acgtcgttcg
     1801 agcgctggca gcgacccagg accatctcgt gcggcttctc gccaacgaga tcgccgcgcg
     1861 cgtcgagatc ggcgagaatc gccgtccgcg caacgtaccg gtccagcccg tcgtaggccg
     1921 tgcccgtccc gctgacgaca gccgcgtcgt cgaggatcgt cggcatcgcg aggccatggc
     1981 gcttgccggt ctcgtggtcg tcgtgatcgt gggccggggt gatcttgacc gccccggtcc
     2041 cgaaggccgg gtcgacgacc gcgtcggcga tgatcggcac gtcgcgcgcg acgaacggga
     2101 tccggacccg ccgcccgacg agcgcccggt agcgcgcgtc gtccgggtgg accgcgaccg
     2161 cggtgtcgcc gaggatcgtc tcggggcggg tcgtcgcgac cgtgaccgac tcgtccggaa
     2221 gcggctcgcc ggtcgcgtcg tccacgcggt ggtagcggat cgaccagagc gtcccggtct
     2281 ccggggtcga gacgacctcg agatcgctga cgctggtccg acatcccggg caccagttga
     2341 cgagcgcctc ggtccggtag gcgaggtcgc gctcgtggag cgtcttgaac gcgacccgga
     2401 cggccttcgc cgacacgtcg tccatcgtgt accggagccg gttccagtcg agcgacgcgc
     2461 cgaggcgtcg ttcctggctg acgatcacct cgcgggtggt cgtcacgaag tcctgcatgc
     2521 gctcgaggta gcgctcccgg ccaaggctct gacggctctc gccgtcgcgg gcgatgatcc
     2581 cgtcgaggac gaactgggcc gcgatcgagg catggtcgag gcccggcagg aacaacgccg
     2641 ccttgccggt catccgggcg tggcgggtca tgaggtcctc gaccgcggcg cgctgggcat
     2701 ggccaagatg gagcgagccc gtgatgttcg gcggcggctg gatgatcgtg aacggctcgg
     2761 catccgggtc ggggcgggaa ccggcgccgt cgggggcgaa gacgtcggcg gccagccagc
     2821 gttcgtagat cgcgccctcg acctcgctcg gccggtaggc cttgggcaag ccgcccggag
     2881 gcggcgaggc catcttgtcg gtgggttcgg ttgtcgtcgt gtcggtcatc gtcgtcggcc
     2941 cctgtggaat cgaacgcccc gctcgtccag cggacgaacg gggtcgtggt accacctgct
     3001 ggttcgcgat ccggggcggt ccgctcatcg cgggcgccgg cttccgggtc gcctcttggc
     3061 gcgttatcga gcgccgaccg cacggctcgc gagcgacttc ggtcccgtcg tgcctgcgcg
     3121 gctcgcaccg tcccgcgctc gcttcgtggc cgacccgggc ctactcctct cggtccccgc
     3181 cgtgagggcg ggagtgtacc acgggccccg aggccgtcca gacgaggcga gacggggcac
     3241 ccatcggtcg gatgaccgat gaccgacttc cggcgcccgg cgaacctgtc acccgagggc
     3301 tgccgcccgg ccgcccgcca gaggagtcac caccttggca acgttcatgg acatccacaa
     3361 cgggttcgtc ggggtcagcc ggcagcagct cgtcgaagcc cacgagcgcg atctcgccaa
     3421 cgagcaggcg gaaggggtcc acttcgagaa agcctggctc gatcccatct cgggcaaggt
     3481 cttctgcctc tcgagcggac cgagccggga gagcgtcatg cgcgtccacg agcgggccgg
     3541 ccaccccacg gcgcaagtct acgaggtgcc gatcgaggtc tgagctgagg ctcgggccac
     3601 ccgggtcgat gctgcggggc gcctggcggg cgccgagcgc ggtcacgcct ccgtgtcggg
     3661 ccggtccgcg gcagccgcgt agatcgcacc gaggaaccgg ctcgggacac ggccgacctc
     3721 gcgcacgctc gcgaacccgg ctgcgtccgc caaccgcgcg aacgtccgcg cggagaacgg
     3781 atgcggaacc catgggttgc cgcgatccgc gtcgtactcg acgacgatca gccggccacc
     3841 ggggcggagc tcggcgcgcg cccgacgcag gacgtcgatc gggtcctcga cgaaatggag
     3901 gctgttggcg gcgaggaccc cgtcgaaccg gccggccggg agaggagccc ggaagtcagc
     3961 gacccgggtt cgcaggacgc agcccgggaa ccggagcggg agctcgcggg ccagccgtgc
     4021 gagcgcgccc gcgtcgcgat ccatggccac gatctcgctg cccgccccga gcaggtccgc
//
LOCUS       DATAIW010000049         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_91198, whole genome shotgun sequence.
ACCESSION   DATAIW010000049 DATAIW010000000
VERSION     DATAIW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91198"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..926
                     /locus_tag="VHM48_01760"
     CDS             153..926
                     /locus_tag="VHM48_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754156.1"
                     /translation="MIELRGVTVAYHGRPVLRDVDLVIGAGERVALIGPNGAGKSTLL
                     RAITGLVRPIAGEVRLDGRPIDSLDRGTIARCLAVVPQQAALPFATRVEEVVALGRLP
                     HEDPIRGARPADRAATAAAIERVGLGHLLGRDARELSLGERQLVLLALAVAQAAPVLL
                     LDEPTVHLDLRHQVSTMELLVDLNERDGTAIVAVLHDLGLASLFFERLVLIDRGRLVA
                     DGTPAEVLTDERIRDVFGVDPSIVRRVAPAMAGRSATVT"
     gene            993..2120
                     /locus_tag="VHM48_01765"
     CDS             993..2120
                     /locus_tag="VHM48_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2754157.1"
                     /translation="MRIADFALERFFARWEFAVRHLLCASDVEGWPMADLLALADDET
                     AALWAGLRLGYTEAPGHPLLRAEIASLYDAVGADDVLVFAGAEEAIFCLSNVLLGPGD
                     HAVVTWPGYQSLYEVGRAAGADVSLHELRETDGWALDPERLIASLQANTRLVVVNAPH
                     NPTGMLPSAADWARLTGELADRSIHLLADEVYRFLELEPNDRLTAGADAFERGVSLGV
                     MSKSFAMAGLRIGWLATRDHELLARCAAFKDYTTICSSAPAEILSLIGLRARDVVLAR
                     SRRIVAANLVHLDGFFADHADRLSWVRPRGGSIAFPRLLDDESIDAFAGRLVEAEGVL
                     LLPGSQFGFEGNHFRIGFGREDLPQALAGLERFLGQASDRR"
     gene            2389..3234
                     /locus_tag="VHM48_01770"
     CDS             2389..3234
                     /locus_tag="VHM48_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754158.1"
                     /translation="MDIHLGTERIFVLRERDGGDLIRQQAMDHRTQAFGSGIGSLLSR
                     PKAEDVELVASQRRLEPFWHVSGRATYVYERSRDYAIVASAAEVQEVTILGTTYPVKR
                     PAAGTGSFTVPTLEHCREELAHVLDADARTGARVADAAVVLSGPRDEVSDPATLSADD
                     TIVIPPEQRASFVVRQLLAEMLKPVQADRILEESLTLETIDLCYRPIWAYEFHWRPKD
                     KRGVVEIDAVTGAFRQGGSFLPQLGRVITRDALFDIGADTIGLLVPGGSIAVKVARAA
                     LDKSY"
BASE COUNT          426 a         1225 c         1129 g          513 t
ORIGIN      
        1 ccgctggccg cgctcggcgg tgcggcgctc atggcgttcg ccgacctcgc ggccaggatg
       61 ctcggcgaga tcccggtcgg ggtggtcacc gccgtgatcg gggcgccgtt cttcctgtcc
      121 ctcctccgcc ggacccgcac cgggtacgag ctgtgatcga gctgcgcggc gtcaccgtgg
      181 cctatcacgg ccggcccgtc ctgcgcgacg tcgacctcgt catcggggca ggggagcggg
      241 tggccctgat cggcccgaac ggtgccggca agtcgacgct tctccgggcg atcaccgggc
      301 tcgtccggcc gatcgctggt gaggtccgcc tcgacggtcg gccgatcgac agcctggatc
      361 gcggaacgat cgcccgctgc ctcgccgtcg tgccgcaaca ggcggcgttg ccgttcgcga
      421 cccgggtcga ggaggtcgtc gccctcggcc ggctacccca cgaggatccg atccgcggcg
      481 cccggccggc cgaccgagcc gccacggcgg cagccatcga gagggtcggg ctggggcacc
      541 tgctcggccg cgacgcccgc gagctctcgc tcggcgagcg ccagctcgtc ctcctcgccc
      601 tcgccgtcgc ccaggcggcg ccggtcctcc tcctcgacga accgacggtt cacctcgacc
      661 tccgtcacca ggtcagcacg atggagctgc tcgtcgacct caatgagcgc gacgggaccg
      721 cgatcgtcgc cgtgctccac gatctcggcc tcgcgtcgct gttcttcgag cggctcgtcc
      781 tgatcgatcg cggccggctg gtcgccgacg gcacgccggc cgaggtcctg acggacgagc
      841 ggatccggga cgtgttcgga gtcgatccgt cgatcgtccg gcgggtggcg ccggccatgg
      901 ccgggcggag cgcgaccgtt acctgatcgt ccttcgacgg ctcgtccgcg atccactgtc
      961 catcggcggc ggtgatcggc gatcatcgcc ccatgcgcat cgccgacttc gccctcgaac
     1021 ggttcttcgc ccgctgggag ttcgcggtcc gacatctgct ctgcgcctcg gatgtcgagg
     1081 gctggccgat ggccgacctg ctcgccctcg ccgacgacga gaccgcggcg ctctgggcgg
     1141 gcctccgcct gggctacacg gaggcgcccg gccacccgct cctccgggcc gagatcgcga
     1201 gcctctacga cgccgtgggc gcggacgacg tcctcgtctt cgccggtgcc gaggaggcga
     1261 tcttctgcct ctcgaacgtc ctgctcggac cgggcgacca tgccgtcgtc acgtggcccg
     1321 gctaccagag cctctacgag gtcggacggg cggccggggc ggatgtgagc ctccacgagc
     1381 ttcgcgagac agacggctgg gcgctcgacc cggagcggct catcgcgtcg ctccaggcga
     1441 acacccgtct cgtcgtcgtc aacgcgccgc acaacccgac cgggatgctc ccgtcagccg
     1501 cggactgggc ccgcctgacc ggcgagctcg ccgaccgcag catccacctc ctcgccgacg
     1561 aggtctaccg cttcctcgag ctcgagccga acgaccggct cacggccggc gccgacgcgt
     1621 tcgagcgcgg cgtctcgctc ggggtgatgt cgaagtcgtt cgcgatggcc gggctgcgga
     1681 tcggctggct ggcgacccgc gatcacgagc tgctcgcccg ctgcgccgcc ttcaaggact
     1741 acacgacgat ctgctcgtcg gcgccggcgg agatcctgtc gttgatcggg ctgcgggcgc
     1801 gcgacgtcgt cctggcacgc tcccgccgga tcgtcgcggc caacctcgtc cacctcgacg
     1861 gcttcttcgc cgaccacgcc gatcgcctgt cgtgggtccg cccgcgcggc ggctcgatcg
     1921 cctttccgcg cctcctcgac gacgagtcga tcgacgcgtt cgccgggcgg ctcgtcgagg
     1981 ccgagggcgt cctgctcctg ccgggctcgc agttcgggtt cgaagggaac catttccgga
     2041 tcggcttcgg ccgcgaggac ctgccgcagg ccctcgccgg cctcgagcga ttcctcggtc
     2101 aggcgtccga tcgacgctga tccgccgcat cgagcgacgt gtcagagccg tcgagggtcg
     2161 tctccaacgt cgaccggcga cggccgacca ccgaaccgcc agggacgatg ggtcgatcga
     2221 tcgtctcgtc gccctccgca cgggtgcggc gcgggcggcc gattcgacct gacgcaccac
     2281 gccccgcggc ccttgcgttc tgctccatca tgggcaccga tcgatgacga tcggacggca
     2341 cggggtccgt cggacgccgt cgtctgcttg gtgaggggtt cgacggtcat ggacatccat
     2401 ctcgggaccg agcggatctt cgtcctccgc gagcgcgacg gcggcgatct catccgacag
     2461 caggcgatgg accaccggac gcaggcattc ggatcgggta tcggctcgct gctgagccgt
     2521 cccaaggccg aggacgtcga gctcgtcgcg agccagcgcc ggctcgagcc gttctggcac
     2581 gtgtccgggc gcgcgacgta cgtctacgag cggagtcgcg actacgccat cgtcgcgagc
     2641 gccgcggaag tccaggaggt gacgatcctc gggacgacct acccggtcaa gcgaccggcc
     2701 gcgggcactg gctcgttcac ggttcccacg ctcgagcatt gccgcgagga actcgcccac
     2761 gtcctcgatg ccgatgcccg aaccggcgcc cgggtcgccg acgcggccgt cgtcctgtcc
     2821 ggcccgcgcg acgaggtcag cgatccggcg acgctgtcgg ccgacgacac gatcgtcatc
     2881 ccgccggagc agcgcgcctc gttcgtcgtg cggcagctgc tcgccgaaat gctcaagccg
     2941 gtccaggccg atcggatcct cgaggagtcg ctgaccctcg agacgatcga cctctgctat
     3001 cgtccgatct gggcctacga gttccactgg cgcccgaagg acaagcgcgg cgtggtcgag
     3061 atcgatgcgg tgaccggggc attccgccag ggtggctcgt tcctgcccca gctcggccgg
     3121 gtgatcactc gcgacgccct gttcgacatc ggcgccgaca cgatcgggct gcttgtcccg
     3181 ggcggaagca tcgcggtgaa ggtggcgcga gccgcgctgg acaagtccta ctgacaccgc
     3241 gtcccccaaa cccggggcga gtcccgcacg actcagaaac ggggcggccc tgc
//
LOCUS       DATAIW010000050         6101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_268958, whole genome shotgun sequence.
ACCESSION   DATAIW010000050 DATAIW010000000
VERSION     DATAIW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268958"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHM48_01775"
     CDS             <1..275
                     /locus_tag="VHM48_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754159.1"
                     /translation="GSMFVAAWWLITSPSASLKVRETADRIKARMTGRETGLQRFDDE
                     ADLGSLLPNPDETRPSPEAETFPATFAELGETVSAGNGSMSDRAGV"
     gene            complement(420..869)
                     /locus_tag="VHM48_01780"
     CDS             complement(420..869)
                     /locus_tag="VHM48_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX2754160.1"
                     /translation="MNKHPVNVKMVDEAPRGARIADRVTGFMGSWPFIIVQSVIVAIW
                     LVGNIYLLSKPFDPYPFILLNLAFSTQAAYAAPLILLAGNRASLRDRMTLEHAAAEAD
                     IEDAQNRQLLEGNVEILKRVEGLEKRILELEASILAAIKDGGARSPG"
     gene            960..2213
                     /locus_tag="VHM48_01785"
     CDS             960..2213
                     /locus_tag="VHM48_01785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754161.1"
                     /translation="MTIEIRPIHEAELPGWLDCVTTGFLERPDVDKIADEVRTHWDLN
                     RALAAVEGGRIVGTARTWPTELTVPGNARIKAAALTAVTVRPTHRRRGLLRGLLAAEH
                     AAARDRGEVASILYASEYPIYGRFGYGPATQTCGWTVDLAATGFHEAAGGRSGTVDWL
                     PVDESAVDPIRDVFEAWRITQPGEIWRRPITWLSDLGLAGAAWGDAWKGFLVVHRDEA
                     GTIDGYARYHADAKWEHRQPRHALIVDELQGLTGEAQAALWRFVAGVDLAVTIKAERR
                     SRADRLPWLLTNARAAETFESGDGMWAKLHDIPSALQTRTYERTGSLVLEVVVRDAGE
                     DDATARRVRVALDVSPDGARAVETDQSPDLTIDGSALGAAYLGGTRLRDAVLARGWDE
                     HRPGALAEADLLLATRDAPWCSTFF"
     gene            2345..3157
                     /gene="modA"
                     /locus_tag="VHM48_01790"
     CDS             2345..3157
                     /gene="modA"
                     /locus_tag="VHM48_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2754162.1"
                     /translation="MTRIETAARVGVLVVVLAGCTSRATASATGAASPAANLTIYGAA
                     SLKSALDKAKPAWEAAHPGSTLTIATDSSAALETQIEQGAPADVFLSADTTNPKKLAD
                     KGLAAGAAVSFAGNKLTVIVPTANPAAIKSPADLAKAGVKVIAAGDAVPITNYATQLV
                     ANLAKESGYPSDYVAAYAANIASKEDNVKAVVAKIELGEGDAGIVYVTDAKASTKVVT
                     IDVPDAANVPATYAGVVVKASPNAAAAKAFLDWLAGPDGQAILAGFGFLPPS"
     gene            3154..4005
                     /locus_tag="VHM48_01795"
     CDS             3154..4005
                     /locus_tag="VHM48_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754163.1"
                     /translation="MTRLVDTHARRRASAGSAWGHRSVAAVASLIALFLGLPVVALVG
                     RALLDGSLAASLTSRVVLDALLLSLVTTAVSLVLTVVFGLPLAFVLARRSFPGKGWLE
                     AIIDLPIVLPPSVAGLALLIVFGRRGVLAEPFGVLGIAIPFTTVAVILAQTFVSAPFF
                     IRSARTGIASVDRDLEDAARADGASERQLFRSITVPLASAALAAGLVMTWARSLGEFG
                     ATIMFAGNVAGRTQTLPLVVYGEFQQGNLDSSIAAAAILVLAAFGVLVAVRVFHWSRV
                     LDARGIS"
     gene            complement(4173..4643)
                     /locus_tag="VHM48_01800"
     CDS             complement(4173..4643)
                     /locus_tag="VHM48_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754164.1"
                     /translation="MSDDGEHGRSCPWCSAVAPADATTCPACGAALAQRESIGNLVIP
                     GVTTVDPALQAMDGQPIHLRGPSPTQGMANGVMVAAVIGGPLGLAMIGGVAAGAAVEY
                     LSAGRDRQTGPDRLDDVGRPSPSTLLALERLERDASGDVAGDEATDPWRDEPRR"
     gene            4812..>6101
                     /gene="sufB"
                     /locus_tag="VHM48_01805"
     CDS             4812..>6101
                     /gene="sufB"
                     /locus_tag="VHM48_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2754165.1"
                     /translation="MTATRDLVADQREQFAFHDDIDYLAQTKRGLTRATVEEISGFKN
                     EPDWMLQYRLRAYEHFMKRPLPTWTDGLDRINFDNIIYYRKPSEREEKSWDDVPDKIK
                     QTFERLGIPEAERKFLAGVGAQYDSEVVYHSVKEELSKLGVVFMGTDQALIEYPEIFR
                     KHFGTVVPAEDNKFSALNAAVWSGGSFVYVPKGVEVPLPLQAYFRINGENTGQFERTL
                     IVVDEGAKVHYIEGCTAPIYATESLHVAVVEVVALAGSNVRYTTIQNWSNDVYNLVTK
                     RAHAYENSRVEWIDANTGSRKTVKYPSIYLRGENASADIISVAVAGKGQHQDTGAKAV
                     HLAPNTKSRIVSKSVSKDGGRATYRGHLKVSPGATNVVASVRCDALMLDDISRSDTYP
                     YIDIQEDDTTMTHEATVGKVSAEQVFYLMSRGLTENEA"
BASE COUNT          922 a         2185 c         2052 g          942 t
ORIGIN      
        1 tcggctcgat gttcgtcgcc gcgtggtggc tgatcacctc gccgagcgcc tcgctcaagg
       61 ttcgcgagac cgccgaccgg atcaaggccc ggatgacggg ccgggagacc ggtctccagc
      121 gcttcgacga tgaggccgac ctcggcagcc tgctcccgaa ccccgacgag actcgaccgt
      181 cccccgaggc cgagaccttt cccgccacct tcgccgagct cggcgagacc gtcagcgcag
      241 gtaacggctc gatgagcgat cgggcgggtg tctgaagccc cgtgtccgaa gcccttctga
      301 accgggccgg tctgggtcgg cacgcgcttc gcgcgggacg acccggccgt gcgccgggtc
      361 gttcgcttgg cggcgagcga cgtgacggcc cgcagctccg ccgcggaccg gcggtccgct
      421 caccctggag accgagcccc gccgtccttg atcgccgcca ggatcgacgc ctcgagttcg
      481 aggatccgct tctccaggcc ctccacccgc ttgagaatct ccacatttcc ttcaagtagc
      541 tgccggttct gggcgtcctc gatgtcggcc tcggcggcgg catgctccag cgtcatccgg
      601 tcgcgcagcg acgcccggtt tccggccagc aggatcaggg gcgcggcgta ggcagcctgg
      661 gtcgagaacg cgaggttcag gaggatgaac gggtacggat cgaacggctt cgagagcaga
      721 tagatgttgc cgacgagcca gatcgccacg atgacgctct ggacgatgat gaacggccag
      781 ctgcccatga agcccgtgac ccgatccgcg atccgggcgc cacgtggtgc ctcgtcgacc
      841 atcttcacgt tcaccgggtg cttgttcacc gggttcctgc gctgacgcgc cacggcggcc
      901 tccgctgatc tcgaccggga tgtgattcgg tccgctcttc ggctgctatt gtccgcccga
      961 tgacgatcga gatccggccg atccatgagg ccgagcttcc gggctggctc gattgcgtca
     1021 cgaccgggtt cctcgagcgg ccggacgtcg acaagatcgc cgacgaagtc cggacgcact
     1081 gggacctcaa ccgggcgctt gcggctgtcg aaggcggtcg catcgtcggg acggcccgga
     1141 cctggccaac cgagctgacc gtgcccggga acgcccggat caaggcggcc gcgttgaccg
     1201 ccgtgaccgt tcgcccgacc catcgccgac gcgggctgtt gcgcgggctg ctcgctgccg
     1261 agcatgccgc cgcccgcgat cgtggcgagg tcgcctcgat cctctacgcg tccgagtacc
     1321 cgatctacgg ccggttcggc tacggacccg ccacccagac gtgcggctgg actgtcgacc
     1381 tggccgcgac cgggttccac gaggcggccg gcggccgcag cgggaccgtc gactggcttc
     1441 ccgtcgacga gagtgcggtc gatccgatcc gcgacgtgtt cgaggcctgg cgcatcaccc
     1501 agccgggcga gatctggcgg cgcccgatca cgtggctgtc cgatctcggg ttggccggcg
     1561 cggcctgggg ggacgcctgg aagggcttcc tcgtcgtcca tcgcgacgag gcgggcacga
     1621 tcgacggcta cgcccggtac cacgccgacg cgaagtggga gcatcgccag ccgcgccacg
     1681 cgctgatcgt cgacgagctc caagggctca ccggcgaggc gcaggccgcg ttgtggcggt
     1741 tcgtggccgg cgtcgacctg gccgtgacga tcaaggcgga gcggcgcagt cgggctgatc
     1801 gcctgccgtg gctactgacg aacgctcgag ccgcggagac gttcgaatcg ggcgacggca
     1861 tgtgggccaa gctccacgac atcccaagcg ccctgcagac gcgaacgtac gaacggacgg
     1921 gttcgctcgt cctcgaggtc gtcgtgcgcg acgcgggcga ggacgacgcg acggctcgtc
     1981 gggtccgggt ggcgctcgac gtctcgcccg acggagcccg ggccgtcgag acggaccaat
     2041 cgcccgacct cacgatcgac ggcagcgccc tcggcgcggc ctacctcggt ggcacccggc
     2101 tgcgcgatgc ggtcctggcc cggggctggg acgagcaccg acccggcgcc ctcgccgagg
     2161 ccgacctgct gctggccacc cgcgacgctc cgtggtgctc gacgttcttc tgagtcgacc
     2221 ccggccggtc gggtgacatc tctggtgacg accgccgtcc atcagtagac atgtgagcca
     2281 atcctgacca tactccctcg atatctgcgg gttgttcgca gcggctgtcg aggagatgcc
     2341 gaccgtgacc cgaatcgaga ccgccgcccg agttggcgtg cttgtggtcg ttctcgccgg
     2401 ctgcacctcg cgcgccacgg cgtccgcgac cggcgcggcc agcccggcgg ccaacctgac
     2461 gatctacggc gcggcctcgc tcaagagtgc cctcgacaag gcgaagccgg cgtgggaagc
     2521 ggcccacccg ggctcgacgc tgacgatcgc gaccgactcg tcggccgccc tcgagaccca
     2581 gatcgagcag ggcgccccgg ccgacgtgtt cctgtctgcc gacacgacca acccgaaaaa
     2641 gctcgctgac aagggcctcg ccgcgggggc cgccgtgagc ttcgccggca acaagctgac
     2701 cgtcatcgtg cccacggcaa atccagcggc gatcaagtcg ccggcggacc tcgcgaaggc
     2761 gggcgtcaag gtgatcgccg ccggcgacgc ggtgccgatc acgaattacg cgacgcagct
     2821 cgtcgccaac cttgcgaagg agtccgggta tccgtccgac tacgtggcgg cctacgccgc
     2881 gaacatcgcc tcgaaggagg acaacgtcaa ggcggttgtc gccaagatcg agctcggtga
     2941 gggcgacgcc gggatcgtct acgtgaccga cgcgaaggcc tcgacgaagg tcgtcacgat
     3001 cgacgttccc gacgccgcca acgtccctgc gacgtatgcg ggggtcgtcg tgaaggcgtc
     3061 tcccaacgcc gcggccgcca aggccttcct cgactggctc gccggacctg acggccaggc
     3121 gatcctggcc ggattcgggt tcctgccgcc atcgtgacgc ggctcgtcga cacgcacgcg
     3181 cgccgccggg ccagcgccgg atcggcctgg ggccaccgat ccgtcgccgc cgtcgcgagc
     3241 ctcatcgctc tgttcctggg gttgccggtc gtggcgctcg tcggacgggc gctcctcgac
     3301 ggctcgttgg ccgcctcgtt gacttcgcgt gtcgtcctcg acgcgctgct cctgagcctc
     3361 gtcacgaccg cggtcagcct cgtgctgacg gtcgtgttcg gcctgccgct ggcgttcgtc
     3421 ctcgcccgtc ggtcgttccc gggcaagggc tggctcgagg cgatcatcga cctgccgatc
     3481 gtgctgccgc cgtcggtcgc cggtctcgcg ctcctgatcg tgttcgggcg acgtggcgtg
     3541 ctggccgagc cgttcggtgt cctcggaatc gcgatcccgt tcaccaccgt cgcggtgatc
     3601 ctcgcccaga cgttcgtgtc ggcgccgttc ttcatccgct cggcgcgaac cggaatcgcc
     3661 tcggtcgacc gcgacctcga ggacgcggca cgggccgacg gcgcgtcgga gcggcagctc
     3721 ttccgctcga tcaccgtccc gctggcgagc gcggcgctgg ccgccgggtt ggtgatgacc
     3781 tgggcgcgct cgctcggtga gttcggggcc acgatcatgt tcgcgggcaa cgtcgcgggc
     3841 cggacgcaga cgctgccgct cgtggtctac ggcgagttcc agcagggcaa cctcgactcg
     3901 tcgatcgcgg cggcggcgat cctcgtcctc gccgcgttcg gcgtcctcgt cgcggtccgg
     3961 gtcttccact ggagccgcgt cctcgacgct cgcgggatca gctgaccacg aggccggccc
     4021 gtctgaaggc gttcggtgcg cccggcgtgc aaccggaccg gcggcaaggg cgcgcaaccg
     4081 ctgtctgctg gcgttccgcg ccgatcatcg gcgcggtcgg ccgggagatc gagccgcccg
     4141 tcatcgcgtc catgtcgatc gcggtaagcg ctctaccttc gcggttcgtc gcgccatggg
     4201 tcggtggcct cgtcgccggc gacgtcaccc gacgcatccc gctcgaggcg ttcgagtgcg
     4261 agcagcgttg acggactcgg ccgaccaaca tcgtcgaggc gatccggacc ggtctgtcga
     4321 tcccgtccgg ccgacaagta ctcgactgcg gcgcccgccg ccaccccgcc gatcatcgcc
     4381 agcccgagcg gtccgccgat gaccgccgcg accatgacgc cgttggccat cccctgggtc
     4441 gggctcggac cgcgcaggtg gatgggctgg ccgtccatcg cctggagcgc gggatcgacc
     4501 gtcgtcacgc ccgggatgac caggttgccg atcgactcgc gctgggcgag ggcggcgccg
     4561 caggcgggac acgtcgtggc gtccgcgggc gccacggccg agcaccacgg gcaggatcga
     4621 ccgtgttcgc cgtcgtcgct catcacccct ccatggctgg ctgttgcgat cctacgcccg
     4681 ggcgcaacgc cggtcttcgg cccccgcgcg acccgtcgtt gacgcctcgg cggttaattc
     4741 cgataatgtg actcgggatt acgaaccgac ccgtgtccgc gcccgagggg cgcccctctc
     4801 cgggagaccg aatgaccgcg acgcgcgacc tcgtcgccga ccagcgcgaa cagttcgcgt
     4861 tccacgacga catcgactac ctcgcccaga cgaagcgcgg cctgacgcgt gcgacggtcg
     4921 aggagatcag cggcttcaag aacgagccgg actggatgct ccagtaccgt ctccgggcgt
     4981 acgagcactt catgaagcgc ccgctgccga cctggaccga cggtctcgat cgaatcaact
     5041 tcgacaacat catctactac cgcaagccct ccgagcgtga agagaagtcg tgggacgacg
     5101 tccccgacaa gatcaagcag acgttcgaac ggctcggcat cccggaggcg gagcggaagt
     5161 tcctcgccgg cgtcggcgcc cagtacgact ccgaggtcgt ctaccactcg gtcaaggaag
     5221 agctctccaa gctcggcgtc gtgttcatgg gcaccgacca ggcgctcatc gagtaccccg
     5281 agatcttccg caagcacttc gggaccgtcg tcccggccga ggacaacaag ttctccgccc
     5341 tgaacgcggc cgtctggtcg ggcggctcgt tcgtctacgt tccgaagggc gtcgaggtcc
     5401 cgcttcccct ccaggcctat ttccggatca acggcgagaa caccggccag ttcgagcgga
     5461 cgctgatcgt cgtcgacgaa ggcgccaagg tccactacat cgagggctgc accgccccga
     5521 tctacgcgac cgagtcgctc cacgtcgcgg tcgtcgaagt cgtcgccctg gcgggctcga
     5581 atgtccgcta cacgacgatc cagaactggt cgaacgacgt ctacaacctc gtcaccaagc
     5641 gcgcccacgc ctacgagaac tcgagggtcg agtggatcga cgcgaacacg ggctcgcgca
     5701 agaccgtgaa gtacccgtcg atctatctcc gcggcgagaa cgcctcggcc gacatcatca
     5761 gcgtcgcggt cgccggcaag ggccagcacc aggacaccgg cgcgaaggcc gtccacctcg
     5821 cgccgaacac gaagagccgg atcgtctcga agtcggtttc gaaggacggc ggccgggcga
     5881 cctaccgcgg ccacctcaag gtgtcgccgg gcgcgaccaa cgtcgtcgcc agcgtccgct
     5941 gcgacgcgct gatgctcgat gacatcagcc ggagcgacac gtacccctac atcgacatcc
     6001 aggaagacga cacgacgatg acccacgagg ccaccgtcgg caaggtcagc gcggagcagg
     6061 tcttctacct gatgagccgc ggcctgaccg agaacgaggc g
//
LOCUS       DATAIW010000051         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_150834, whole genome shotgun sequence.
ACCESSION   DATAIW010000051 DATAIW010000000
VERSION     DATAIW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150834"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHM48_01810"
     CDS             <1..313
                     /locus_tag="VHM48_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754166.1"
                     /translation="ANGCRQVTELVPSGLAPVGAWSHWTDPDAEVDPATADVILPYDQ
                     SGSQVFFCVGPDAARRAFTLRYVARVVTPGTYVWEPAVAQSGSAEDIANLAPATSITI
                     R"
     gene            407..1828
                     /locus_tag="VHM48_01815"
     CDS             407..1828
                     /locus_tag="VHM48_01815"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX2754167.1"
                     /translation="MTIEPRPAARIERDSMGEMEVPADALYGASTQRAVLNFPISGRR
                     FPREFIEALALIKLAAAETNAELGLLDGDVAAAIAAAAGEVAAGGHDDQFPIDIYQTG
                     SGTSTNTNMNEVVAHLATSRLAAAGSATKVHPNDHVNKCQSSNDVIPTALQLSAAKAI
                     EEALLPGLETLRAALEAKADELWPVVKTGRTHLQDATPIRLGQEFRGYAGQLAESIRR
                     ARAAQAELLVVPLGGTAVGTGINAHPAFAAGATGRLSRRTGLDVRETANHFHAQASLD
                     AAIAAHGALRTIALSLWKIASDIRLMGMGPRAGIAEIALPETQPGSSIMPGKVNPVIV
                     ESLTMVVARVLGNDTTVAFGQTGSFLELNVMLPVTAAALLESIGLLAAAARNFADRAI
                     SGLTATDRGPALVEQGLMLATALAPEIGYDAAAALAKEALKTGRTIRDLATERGIAPG
                     RLDELLDPASMTEPGLGGGPAGG"
     gene            complement(1832..2542)
                     /locus_tag="VHM48_01820"
     CDS             complement(1832..2542)
                     /locus_tag="VHM48_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754168.1"
                     /translation="MILRRDRPSGSQPAGARLLWLYPARWRRRYGAEMLATLEERRIG
                     PRDRLDLVRGALDAWLHHPSRVPAVAALVAGGLWTVAGAAVAAQPVPPDWPGYLLETL
                     PIAIVAAAMGGLATIGCWARRSDTGGRSGTIAILLSTVGHLAWVVALVAAWSGAGYGI
                     ATMAAQAFGALGAVLVGLLLLRSGDERIGSVLVVAPALMLFGWPIAWLGFGFAWTLVG
                     VLLLARPEPDEPLPSSFA"
     gene            complement(2539..3207)
                     /locus_tag="VHM48_01825"
     CDS             complement(2539..3207)
                     /locus_tag="VHM48_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2754169.1"
                     /translation="MGTRPLPRIVPRAGPLEGHVLQRLRSRPTSGISRRALLRRSLGA
                     AVGLTIVEGLGGTVGFLWSASTAASRRVRIGTLDELIALNAGLPIEDGFPAYVPDARA
                     FILTIDPARGWQPGADPTGEGTALNVRALSQRCPHLGCRPNPCVEDFWFHCPCHQSRY
                     DRLGIKAAGLGPAARGMDRLAVEVDRNGVLTVETGRISLGPLPVALGQPGLIPPRVER
                     GCGG"
     gene            complement(3304..3585)
                     /locus_tag="VHM48_01830"
     CDS             complement(3304..3585)
                     /locus_tag="VHM48_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754170.1"
                     /translation="MLTQPARPGSGRAIAWAAATVVGLAAGGFAFHFPGSVGARRDPS
                     ALVFGTLLGGVSGLVAGLLQAIALRGAVARGRWRTSSRVSSPARSSRRS"
     gene            complement(3621..>4070)
                     /locus_tag="VHM48_01835"
     CDS             complement(3621..>4070)
                     /locus_tag="VHM48_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754171.1"
                     /translation="VSGVAIASSRAIGPDGYQDNGSAVPLLIAGMFLTATAALLMATE
                     RTPQRTAAVMVGGSLLMVLSWPILIVGFFAYIGATIALGAQLLARGQLAGLALIAIGL
                     ILPSFNTETDWALAAVPVGIFWIAFGAAMWRSGTPLTTTVAARGVGG"
BASE COUNT          627 a         1498 c         1468 g          477 t
ORIGIN      
        1 cgcgaacggc tgccgccagg tgaccgagct cgtcccgagc ggcctggcgc cggtcggtgc
       61 gtggtcgcac tggaccgatc cggatgccga ggtggacccg gcgaccgccg acgtcatcct
      121 gccgtacgac cagtcgggct cccaggtctt cttctgcgtc ggaccggatg ccgcgcggcg
      181 ggccttcacc ctgcgctacg tcgctcgcgt cgtgaccccg ggcacgtacg tctgggagcc
      241 cgcggtcgcc cagtccggca gcgccgagga catcgccaat ctcgctcccg cgacctcgat
      301 cacgatccgg tagcccgaag gccggcgccg ggctcgagct cgtctcgggc ccggcgtcgc
      361 tgcgcccggg tccgttcgcc ggtcgtcggc cgcctacaat cggggcatga cgatcgaacc
      421 ccgtccggcg gcccgcatcg agcgcgactc gatgggcgag atggaggtcc cggccgacgc
      481 gctctacggt gcctcgaccc agcgggcagt cctgaacttc ccaatctcgg gccggcgctt
      541 cccgcgtgaa ttcatcgagg ccctcgccct gatcaagctg gccgcggccg agacgaacgc
      601 cgagctgggc ctgcttgatg gcgacgtcgc tgcggcgatc gcggcggccg ccggcgaggt
      661 tgcggcgggc ggccacgacg accagttccc gatcgacatc taccagaccg ggtcgggcac
      721 gtcgacgaac acgaacatga acgaggtcgt cgcccatctc gcgaccagcc ggctcgcggc
      781 cgccggttcg gcgacgaagg tccatcccaa cgaccacgtc aacaaatgcc agtcgtcgaa
      841 cgacgtgatc ccgaccgcgc tccagctgtc ggcggcgaag gcgatcgagg aagcgctgct
      901 gccgggtctc gagaccctcc gcgcggcgct cgaggccaag gcggatgagc tctggccggt
      961 ggtcaagacg ggccggaccc acctccagga cgcgaccccg atccgcctcg gccaggagtt
     1021 ccggggctac gccggccagc tcgcggagtc gatccggcga gctcgcgccg cgcaggcgga
     1081 gctgctggtc gtgccgctcg gaggaaccgc cgtcgggacc gggatcaatg cccacccggc
     1141 ctttgcggcg ggggccacgg gacgcctgtc gaggcggacc ggcctcgacg tccgcgagac
     1201 cgccaaccac ttccacgccc aggcgtcgct cgacgccgcg atcgcggccc acggcgccct
     1261 caggacgatc gctctgtcgc tctggaagat cgcgagtgac atccgcctga tgggcatggg
     1321 cccgcgggcc ggcatcgccg agatcgccct gccggagacc cagccgggct cgtcgatcat
     1381 gccgggcaag gtgaacccgg tgatcgtcga gagcctgacg atggtcgttg cccgggttct
     1441 gggcaacgac acgaccgtcg ccttcggcca gaccgggtcg tttctcgagc tcaacgtgat
     1501 gctgccggtc acggccgcag cgctgctcga gtcgatcggc ctcctggcgg cggccgcccg
     1561 taacttcgcc gaccgcgcca tctccggcct gaccgccacc gaccgtgggc cggccctcgt
     1621 cgagcagggc ctgatgctcg cgacggcgct cgccccggag atcggctatg acgcggcagc
     1681 ggctctcgcc aaggaggcgc tcaagaccgg ccggacgatc cgcgacctcg ccaccgagcg
     1741 tggaattgcg cccggccgcc tcgacgagct cctcgatccc gcgtcgatga ccgagcccgg
     1801 cctcggcggc ggaccggcgg gcggctgagg gtcaggcgaa gctcgacggc agcggttcgt
     1861 ccggctctgg tcgcgccagg aggaggacgc cgacgagcgt ccaggcgaag ccgaacccga
     1921 gccaggcaat cggccacccg aacagcatga gcgccggagc gacgacgagc accgaaccga
     1981 tgcgctcgtc gcccgaccgg aggagcagga ggccgacgag caccgcgccg agggcgccga
     2041 acgcctgggc cgccatcgtc gcgatgccgt agccagcccc ggaccaggct gcgacgaggg
     2101 cgacgaccca cgcgaggtga ccgaccgtcg agaggaggat cgcaatcgtg cccgagcggc
     2161 cgcccgtgtc gctccgtcgg gcccagcagc cgatcgtcgc aagaccgccc atcgcggccg
     2221 cgacgatggc gatcgggagc gtctccagca ggtagccggg ccagtcgggc ggaaccggct
     2281 gggccgcgac ggcggcaccg gcgaccgtcc acagcccgcc cgccacgagc gccgccacgg
     2341 caggcacgcg ggacgggtgg tggagccagg cgtcgagcgc gccgcggacg agatcgaggc
     2401 gatcgcgcgg cccgatgcgg cgttcctcga gggtcgcgag catctcggcg ccatagcgcc
     2461 tccgccatcg cgcgggatac agccagagca gacgagcgcc ggccggctgc gacccggacg
     2521 gtcggtcgcg gcgcaggatc atccgccgca acctcgctcg acccgcggcg ggatcagacc
     2581 gggctggccg agcgcgacgg gcagcggacc cagcgagatc cgcccggtct cgacggtcag
     2641 gaccccgttg cggtcgacct cgaccgccag gcggtccatg ccgcgggcgg ccggcccgag
     2701 gccggcggcc ttgatcccga ggcggtcata gcgcgactgg tgacacggac agtggaacca
     2761 gaaatcctcc acgcacggat tcgggcggca gccgaggtgg gggcagcgtt gcgagagcgc
     2821 ccgcacgttc agggcggttc cctcgcccgt cggatcggcg cccggctgcc aaccgcgggc
     2881 gggatcgatc gtcaggatga acgcgcgcgc gtccggaacg taagccggga agccgtcctc
     2941 gatcggcagc ccggcgttga gcgcgatgag ctcgtccagg gttccgatgc ggacgcgccg
     3001 tgacgccgcc gtggaggccg accacaggaa cccgaccgtc ccgccgagcc cctcgacgat
     3061 cgtcaggccg acggcggccc cgagcgagcg gcggaggagg gcgcgccggg agatgccgct
     3121 cgtcggccgg ctgcgcaggc gctggaggac gtggccttcg agcgggccgg cgcgcggcac
     3181 gatccgtggg agcggtcggg tgcccatcgg ttcgtcaggc ccgtgccggt ctggccgtcg
     3241 ccggaaccgc cggcccgcca gcggtccgca tcgccggggc cgtcccaggg aaccccatca
     3301 cgatcacgac cgccgcgaag atcgcgccgg tgacgacacc cgcgacgagg tgcgccatcg
     3361 accacgggcc accgcgccgc gcagcgcgat cgcctggagg agaccggcga cgagccctga
     3421 cacgccaccc agcaacgtcc cgaagacgag tgccgacgga tcccgcctcg cgcccaccga
     3481 tccggggaag tggaacgcga atccgccggc ggccaggccg acgaccgtgg cggccgccca
     3541 ggcgatggcc cgaccggacc cgggacgggc gggctgcgtc agcatcggtc gctgaggact
     3601 ggtccgggga acgggcgcga tcacccgcca acgcctcggg ccgcgaccgt cgtcgtgagc
     3661 ggcgtgcccg accgccacat cgccgccccg aacgcgatcc agaagattcc gaccgggaca
     3721 gcggcgaggg cccagtcggt ctcggtgttg aacgacggta ggatcagccc gatcgcgatc
     3781 agggcaagtc cggcgagctg tccccgggcg agcagctggg cgccgagcgc gatcgtcgcc
     3841 ccgatgtagg cgaagaaccc gacgatcagg atcggccacg aaagcaccat cagaagggat
     3901 ccgccgacca tgacggcggc ggttcgctgc ggcgttcgtt cggttgccat cagcagcgct
     3961 gcggtcgccg tcagaaacat gccggcgatc aggagcggca ccgcgctgcc attgtcctgg
     4021 tagccatccg gaccgatcgc ccgcgacgag gcgatcgcca cgccactgac
//
LOCUS       DATAIW010000052         7446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1776, whole genome shotgun sequence.
ACCESSION   DATAIW010000052 DATAIW010000000
VERSION     DATAIW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VHM48_01840"
     CDS             complement(<1..1268)
                     /locus_tag="VHM48_01840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754172.1"
                     /translation="MISMGVDPTPSADEVASDRVEDPSIWRNGAFVRLFTAASVSYVG
                     SFVTRTALPLAAIYGLSAGPLELSAIRSFEFLGWLLVGLVAGAWVDRLRRRPVMIAAD
                     LGRAVILVSIPVAAIGGSLTLAHLVVAAFLAAILSVFFNAASGAYLPSIVARSRLVAA
                     NSAMSASASMAEFTGFGLSGVLVQILTAPIAIAVDALSFVGSALVLMTIRHPEPARPP
                     AADREPVLREIREGIDVVAHSPILRALAIAHAADHLLWGVFGATYLLFAVDEIGLAPA
                     AIGVITAIGGLGSFVGAAMASRVIARLGAGRAMIVGLAGAAIGSALIPLAPTGAVLIG
                     GALLVGQQIIGDASGTVYEVVEASVTQTIVHDRVLGRVKATVSVITTVTALAGSILGG
                     VIGAAFGLRAALIVGIAGAVIAVIVVWRSPA"
     gene            complement(1307..2059)
                     /locus_tag="VHM48_01845"
     CDS             complement(1307..2059)
                     /locus_tag="VHM48_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX2754173.1"
                     /translation="MDVREQLCYSDSYARTVEARVATVDESGEAPLVVLDRTVFYPGG
                     GGQPSDRGLLLRASDGRTWTVRSARKSGRDIVHELEPGPHGPPARDDVVTVDLDWARR
                     YALMRTHTALHALCGVVWRDYGALVTGGNMEPGGGRMDFEFERMSGDLVDAIEATVNT
                     ELAHKRDVRVDVLPRAEAFAIPDLIRTKINLLPEGIAEIRTIEIVGLDLQADGGTHVS
                     NTSEVGGIKVTGYESKGRINKRIRLELVDPEG"
     gene            complement(2132..2668)
                     /locus_tag="VHM48_01850"
     CDS             complement(2132..2668)
                     /locus_tag="VHM48_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2754174.1"
                     /translation="MRCLPALFLIALAIGALAACSAGAAPGWTYAPAASATPVASGAA
                     SGEPSVGPSVAPSSASSVAPSVAPSAGESAAASGGTGATLTVTAPVGAASSGFQPTTL
                     DATANAAFTIHFDSQDPQPHNVQLKGAGGEVALGGDTAFFQGPGTRDYAVPALPAGTY
                     TYYCMVHPGTMTGTLTAK"
     gene            complement(2752..6201)
                     /locus_tag="VHM48_01855"
     CDS             complement(2752..6201)
                     /locus_tag="VHM48_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2754175.1"
                     /translation="MPATMNSARFVGRDAAFVRLAPALEAASDGEATTVLLDGPGGVG
                     VSRFVAELGRRVAGLAEPFVVVRGRSFRPGSDDPYGAIIRALRPVFHAVDDAELAAIV
                     GPAAEDVVRLFPEVVGRLSSAGALPGRPTSTALERRQGRVLEGLLGVVGRLAERQPVL
                     FVIEDLHDADAGSRAFVSFMSRIRRAQRVCLIGTWQPDELTRDHPLSRTLAEMAAVQE
                     RGPARISIPPLERAELAELVQAIEGERPTASALVLVADRSRGLPLVAEELLGARRELS
                     DTSLTGSFADIVIARLARYGPECRRVLRLLALAGRPIDRDELAETAAAFELTADRLPP
                     RSSTRPRHGDGALDPDLAAGLEEALGAGILVEETEGVAFRHEHIRRAAAADLLPRLRI
                     RHHLALAAGLVAQPNEAASHWLAAHVPDRAFAAAVDAAGRAEAAHAPEDALAALEVAL
                     ALVDPSARAGEPVTIPLPEAPPDGGDAQAGADGSLRTRTPARSTRGERATTADARSED
                     GRARADAIREVATPLQLRAAESAFAAGRPARAVAYLEAILGSFDERRDRVALGLLHER
                     LGRYRRAAGDRTGALVALERAVDLVPDGPTLERATVLAALAQATMLDGSFSRAETLAR
                     DAMRIGAACGHEGEAIVVHATTTLGVALGWGDAPEAGVALLEEARGLAEQAGDADEVF
                     RVYANLTTVLDLVGRRPEAVEVAYEGIEASRRAGLEAVYGNFLRGNASDSLYLLGRWA
                     ESSAISATALEWSPAGVAFVRPIDSLAVVEIETQAGEAAGRRLGQMLVELETVSDAQH
                     AVPIYRAAASLALWQGDHADAGRAAGRGWDLVKDSGDWSLLAKMAATVAEVDSMAAAD
                     AGARRDIATIASIRGRSRNVVKTATASIARSGVGPTIGSRREADAWLALATAHRDRLE
                     GRDDPAAWDRLAAAWEALANPYEVAKARWRQAEAILASGEGRAGRGHARPVLEEAAAI
                     GLTLGARPLLREIRQLAGRAMIRLPESVDALLDAPDPRLAGGASAALGDGGERGDGTL
                     VAVGPGPDGQAAGAGNGSTHGNGTGGESAIIRGVVGEAPVSKRDTFGLSRREREVLAL
                     MAEGRTNREIGERLFISQKTVGVHVGNILAKLGVSGRVEAAAVAIRLGLTEPSAAGTS
                     SGR"
     gene            complement(6365..>7446)
                     /locus_tag="VHM48_01860"
     CDS             complement(6365..>7446)
                     /locus_tag="VHM48_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX2754176.1"
                     /translation="ATPSAGVDGTGGSGSGGSGSGSGGTDSTGPSAGPGSGTGGTGAA
                     GGDTTDPTGSGGTGGPGGLGTGTSGGDTGTGANAGQGSGNGQGTGPDGSANGAGPLAG
                     PGTAGNPGAGSAGGDPSTTNGGDAGRGIVTGGPGWGAFASALEALGIDRPPSVTMLPM
                     LVGTSTAMTMAFAFAIFGKKRRDEEPPAPDEVLQANAARGHGSVPGSEIAHGVVRAAS
                     FPAPLDLEAGMPRWRRPSLLEARKADPNRSVASSHRMSFESGVVGAADDRERRTIRYA
                     VVRLLDAPDELRSAEIGQLDRGDEVQLIERSGSYWLVLCPDGRQGWLHKMTLGEVVTD
                     AAPTTFGRDVDDDVLTAFLTARARA"
BASE COUNT         1029 a         2713 c         2777 g          927 t
ORIGIN      
        1 gccggcgagc gccacacaac gatcaccgcg atgaccgccc cggcgatccc gacgatcaac
       61 gccgctcgca gcccgaaggc cgcgccgatg accccgccga ggatcgagcc ggcaagcgca
      121 gtgacggtcg tgatgacgct cacggtcgcc ttcacccggc ccaggactcg gtcgtggacg
      181 atcgtctggg tgacggacgc ctcgacgacc tcgtagaccg tcccggaggc gtcgccgatg
      241 atctgctggc cgaccaggag ggcgccgccg atcagaacgg cccccgtcgg cgcgagcggg
      301 atgagcgcgc tcccgatcgc cgccccggcc agcccgacga tcatcgcccg gcccgctccg
      361 agccgggcga tcacccgcga tgccatcgcc gctccgacga acgagccaag cccgccgatg
      421 gccgtgatca cgccgatggc agccggcgcc agcccgatct cgtcgaccgc gaacagcagg
      481 taggtcgcgc cgaagacgcc ccacagcagg tggtcggccg cgtgggcgat cgcgagcgcg
      541 cgcaggatcg ggctgtgcgc aacgacgtcg atgccctcgc ggatctcgcg caggaccggc
      601 tcccgatcgg cagccggcgg gcgagccggc tccgggtggc ggatcgtcat gaggacgagc
      661 gccgatccga cgaacgacag ggcgtcgacc gcgatcgcga tcggcgcggt gaggatctgg
      721 acgaggacgc cgctcaaccc gaaccccgtg aactcggcca tcgacgcgct cgccgacatc
      781 gcgctgttgg cggcgacgag ccggctccga gcgacgatcg acgggaggta ggcgccgctt
      841 gccgcgttga agaagaccga gaggatggcg gccaggaacg cggcgacgac gagatgagcg
      901 agggtcagcg acccgccgat cgcggcgact gggatggaga cgaggatcac ggcccgcccg
      961 aggtcggccg cgatcatgac cgggcggcgc cggaggcgat cgacccatgc cccggcgacg
     1021 agcccgacga ggagccagcc caggaactcg aagctgcgga tggcggacag ctccagcggc
     1081 ccggccgaca ggccgtagat cgcggcgagc ggcagcgccg tgcgggtgac gaacgagccg
     1141 acgtaggaga cgctggccgc ggtgaagagc cggacgaacg cgccgttccg ccagatcgac
     1201 ggatcttcga cgcgatcgct cgcgacctcg tccgctgacg gcgtcggatc aacgcccatg
     1261 ctgatcatcg gcgcggatca tcccacgccg accgagctcc ggatcgtcag ccctccgggt
     1321 cgaccagctc gagccggatc cgcttgttga tccggccctt cgactcgtag ccggtcacct
     1381 tgatgccgcc gacctcggac gtgttcgaca cgtgggtccc gccgtcggcc tggaggtcga
     1441 gcccgacgat ctcgatcgtc cggatctcgg cgatcccctc gggcaacagg ttgatcttcg
     1501 tccggatcag gtcggggatc gcgaaggcct cggcccgcgg caggacgtcg acccggacgt
     1561 cgcgcttgtg ggcgagctcg gtgttgacgg tcgcctcgat cgcgtcgacg aggtcgccgc
     1621 tcatccgctc gaattcgaag tccatccgcc ccccaccggg ctccatgttg ccgcccgtca
     1681 cgagcgcgcc gtagtcgcgc cagaccacgc cgcacagggc gtggagcgcg gtgtgcgtcc
     1741 gcatcagggc gtagcgtcgg gcccaatcga gatcgacggt gaccacgtcg tcgcgggccg
     1801 gcggtccatg cggccctggc tccagctcgt gcacgatgtc gcggccggac ttgcgggcgg
     1861 accggaccgt ccacgtccgg ccgtcggacg cgcgcaggag cagccctcga tcggacggct
     1921 ggccaccgcc gcccgggtag aacaccgtcc gatcgaggac gacgagcggc gcctcaccgc
     1981 tctcgtcgac cgtggccacg cgggcctcga cggtccgcgc gtacgaatcc gagtagcaga
     2041 gctgctcgcg cacgtccatc gagcggttcc tcgcgacggg ccgggggtgt ccgccgtggc
     2101 gtccccggcc gggcgccgcg aacgtggtcc tctacttggc ggtgagggtc cccgtcatcg
     2161 tgcccgggtg gaccatgcag tagtaggtgt aggtcccggc gggcagggct gggaccgcgt
     2221 agtcgcgggt gcctggtccc tggaagaagg cggtgtcgcc gccgagggcg acctcgccgc
     2281 cggcaccttt cagctggacg ttgtggggct gcgggtcctg gctgtcgaaa tggatcgtga
     2341 acgcggcgtt cgcggtcgcg tcgagcgtcg tcggctggaa cccgctgctc gccgccccga
     2401 ccggtgcggt gaccgtcaac gtcgcgccag tgccgcccga tgcggccgca ctctcaccgg
     2461 ccgagggagc gaccgatggc gccaccgacg aggcggagga cggggcgacg gacgggccga
     2521 cggagggctc gccggacgcc gcgcccgaag ccaccggggt ggccgaggcg gccggggcat
     2581 aggtccagcc gggggcggcg ccggccgaac acgcggccag ggcaccgatg gccagggcga
     2641 tcaggaagag ggcaggcagg caacgcatgt cgtcgggaga cctctgtgaa ctgggcgtgg
     2701 gacccggctt tcccgggacg acgcgtcggg gacgtcgcgt cggacccgaa tctaccgccc
     2761 ggacgacgtt cctgccgcgc tcggctccgt cagcccgagc cggatcgcga cggctgccgc
     2821 ctccaccctg cccgagaccc cgagcttggc gaggatgttg ccgacgtgga cgccgacggt
     2881 cttctggctg atgaacagtc gttcgccgat ctcgcggttc gtccgaccct ccgccatcag
     2941 ggccaggact tcgcgctcgc gccgcgacag cccgaacgtg tcgcgtttgc tgaccggtgc
     3001 ctccccgacg accccgcgga tgatcgccga ttcgccgccg gtcccgttgc catgcgtcga
     3061 gccgttcccc gcgcccgccg cctgcccgtc cgggccgggg ccgaccgcga cgagggtccc
     3121 gtcgccacgc tcaccgccgt cgccgagggc cgctgacgcg ccccccgcca gccgggggtc
     3181 gggtgcgtcg aggagagcgt cgacggactc ggggaggcgg atcatggccc ggccggccag
     3241 ctgacggatc tcgcgcagga gcggccgcgc gccgagggtc agcccgatcg ccgccgcctc
     3301 ctcgagcaca ggccgggcat gaccgcgacc ggcccggccc tcgcccgaag cgaggatcgc
     3361 ctcggcctgg cgccagcgcg ccttggccac ctcgtacggg ttcgcgagcg cctcccaggc
     3421 agcggccagg cggtcccagg cggccgggtc gtcgcggcct tcgagtcgat cgcggtgggc
     3481 ggtcgcgagg gccagccacg cgtccgcctc acggcgagac ccgatcgtcg ggccgacgcc
     3541 ggaccgagcg atcgacgcag tcgcggtctt gacgacattg cgcgaccggc cccggatcga
     3601 ggcgatggtc gcgatgtcgc ggcgcgcgcc ggcgtcggcc gcggccatcg agtcgacttc
     3661 cgcaaccgtc gcggccatct tggccagcag gctccagtcg ccgctgtcct tgacgaggtc
     3721 ccagccacgt ccggccgccc gacccgcatc cgcatggtcg ccctgccaga gcgcgagcga
     3781 cgccgccgcc cggtagatcg ggacggcatg ctgggcgtcg ctcaccgtct cgagctcgac
     3841 gagcatctgg ccgagccgac ggccggcggc ctcgccggcc tgggtctcga tctcgacgac
     3901 cgcaaggctg tcgatcgggc ggacgaaggc gactcccgcg ggactccact cgagcgcggt
     3961 cgcgctgatg gcactcgatt cggcccagcg gccgaggagg tagagtgaat cggaggcgtt
     4021 gccccggagg aagttgccgt agacggcctc gagcccggcc ctgcggctgg cctcgatgcc
     4081 ctcgtaagcg acctcgaccg cctccggccg ccgcccgacc aggtcgagga ccgtcgtcag
     4141 gttggcatag acgcggaaca cctcgtcggc atcgccggcc tgttcggcca ggccgcgcgc
     4201 ctcctcgaga agggccaccc ccgcctccgg tgcgtcgccc cagccgaggg cgacgccgag
     4261 ggtcgtcgtc gcgtggacga cgatggcctc gccctcgtgc ccgcaagcgg caccgatccg
     4321 catcgcgtcg cgggcgaggg tctcggcccg ggagaacgag ccgtcgagca tcgtcgcctg
     4381 ggcgagggcg gcgaggacgg tggcccgctc cagcgtcggt ccgtcgggca cgagatcgac
     4441 cgcccgctcg agcgcgacga gggcgcccgt ccgatcgccg gcggcgcgtc gatagcggcc
     4501 gagacgttca tggagcaggc cgagcgcgac ccgatcgcga cgttcgtcga acgagccgag
     4561 gatcgcctcg agataggcga ccgcacgggc cggccggccg gccgcgaacg ccgactccgc
     4621 ggcgcgcagc tggagcggcg tggcaacctc acggatcgca tccgcccggg cacggccatc
     4681 ctcgctccgg gcgtccgccg tggtcgctcg ttcgcctcgc gtcgagcgcg ccggggtcct
     4741 cgtccggagc gacccgtccg cgccggcctg agcgtcaccg ccatccggcg gcgcctcggg
     4801 caggggaatg gtgaccggct cgcccgcccg ggccgacggg tccacgagag cgagcgccac
     4861 ctcgagtgcg gcgagggcgt cctcgggcgc atgcgccgcc tcggctcggc cggccgcgtc
     4921 cacggcggcg gcgaaggctc gatcgggaac gtgggccgcc agccaatggc tcgctgcctc
     4981 gttcggctgg gcgacgagcc cggccgcgag ggcgaggtgg tggcgaatac gcaggcgcgg
     5041 caggaggtcg gccgcggccg cccgccggat gtgttcgtgg cggaaggcaa cgccctcggt
     5101 ctcctcgacg aggatcccgg ccccgagcgc ctcctcgagg ccggcggcaa gatccggatc
     5161 gagcgctccg tcgccgtgac gcgggcgggt cgaggatcgc ggcggcaggc gatcggcggt
     5221 gagctcgaag gcggcggcgg tctcggccag ctcgtcgcga tcgatcggcc ggccggcgag
     5281 ggcgagcaac cgcaggaccc gccggcattc cggaccgtat cgggcgaggc gggcgatcac
     5341 gatgtcggcg aaggagccgg tcagcgaagt gtcggacagc tcgcggcgcg cgccgaggag
     5401 ctcctccgcg acgagcggca gcccgcgcga gcgatccgcg acgaggacga gggcggaggc
     5461 ggtcggccgc tcgccttcga tcgcctggac gagctcggcg agctcggctc gctccagcgg
     5521 cgggatcgag atccgggccg gtccacgctc ctgcaccgcc gccatctcgg ccagcgtccg
     5581 cgacagcggg tggtcgcggg tgagctcgtc cggctgccac gtgccgatca ggcagactcg
     5641 ctgggcgcgt cggatccggc tcatgaacga gacgaacgcc cggctgccgg cgtccgcgtc
     5701 gtggagatcc tcgatgacga acaggacggg ctgtcgctcg gccagccgtc cgacgacccc
     5761 gagcagccct tcgaggaccc ggccctgacg gcgctcgagg gccgtcgagg tcggacgccc
     5821 cggaagggcg ccggccgacg acaggcggcc gaccacctcg gggaacagcc ggaccacatc
     5881 ctcggccgcg ggtccgacga tggcggcaag ctccgcgtcg tcgaccgcat ggaagaccgg
     5941 ccggagcgcg cgaatgatcg ccccgtacgg gtcgtccgag ccgggccgga aggatcgacc
     6001 gcggacgacg acgaacggtt cggccaggcc ggccacccgg cggcccaact cggcgacgaa
     6061 ccgcgagacg ccgacgccgc cgggaccgtc gaggaggacg gtcgtggcct caccatccga
     6121 cgccgcctcg agcgccgggg cgagtcgaac gaacgccgcg tcgcgtccca cgaaccgcgc
     6181 gctgttcatc gtcgccggca tgacgggcat tctaggggtc cggacagcgg ttccggtgag
     6241 ccggggacga acgcgggccc gttcacaggg tggggatcct catggcgggc cccatcgggg
     6301 cggaccgatc gctgcgcgat ccgttcgagc gaccgccgcg aatctgcccg tgagcggtca
     6361 gcgatcaggc gcgggcgcgg gcggtcagga acgcggtgag cacgtcgtca tcgacgtccc
     6421 ggccgaaggt ggtcggggcg gcgtcggtga cgacctcgcc gagggtcatc ttgtggagcc
     6481 agccctggcg gccgtcgggg cagaggacga gccagtacga gccggagcgt tcgatgagct
     6541 ggacctcgtc gccccggtcg agctggccga tctccgccga gcgcagctca tcgggggcat
     6601 cgaggaggcg gaccaccgcg taacggatcg ttcgccgttc gcggtcgtcc gccgcgccga
     6661 cgacgccgct ctcgaagctc atccggtggc tggacgcgac cgaccggttc ggatcggcct
     6721 tgcgcgcctc gaggagcgat ggccggcgcc agcggggcat ccctgcctcg aggtcgaggg
     6781 gagccgggaa gctggcggct cgcacgacgc catgggcgat ctcgctgccc gggacggacc
     6841 cgtggccccg cgcggcgttg gcctggagga cctcgtccgg tgcgggcggc tcttcgtcgc
     6901 gccgcttctt gccgaagatc gcgaaggcga aggccatggt catcgcggtg ctggtcccga
     6961 cgagcatcgg gagcatggtc accgacggcg gtcggtcgat cccgagggcc tcgagggcgc
     7021 tcgcgaacgc gccccaaccg gggccgccgg tcacgatccc gcggccggcg tcgccgccgt
     7081 tcgtcgtcga tggatcgccg cctgccgaac cggctccggg attgccggcg gttccgggcc
     7141 ccgcgagcgg ccctgcgccg ttggcgctgc catccgggcc ggtgccctgg ccattgcccg
     7201 agccctggcc ggcgttggca ccggtgccgg tgtcaccacc ggaggtgccg gtaccgagcc
     7261 cgcccggacc gccggtgccg ccggatccgg tcggatcggt cgtgtcgccg ccggccgcac
     7321 ccgttccgcc ggtgcccgag cccgggccgg cggacggacc ggtgctgtcg gtcccgccgc
     7381 tgcctgagcc ggaaccaccc gatccggagc cgcccgttcc gtcgactccg gccgacgggg
     7441 tggcat
//
LOCUS       DATAIW010000053         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_1903, whole genome shotgun sequence.
ACCESSION   DATAIW010000053 DATAIW010000000
VERSION     DATAIW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5048)
                     /locus_tag="VHM48_01865"
     CDS             complement(<1..5048)
                     /locus_tag="VHM48_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2754177.1"
                     /translation="MFDHERADDLPVAPSDLDDRGWVQSAAPGTAAHAPSTTRRGPRS
                     GPRRPAWLRQRAVVVTVAVVATALVAGLFAWNGGRLADGVADASQPVASDVAVGSGGP
                     TASGRPGPSASAQPSQGYEPLTGISPAPTALLTARGASGVVVPLDASFRLESTDGTPA
                     STLAAGLTVEPAFSFTVNKVAGDHAVEVAPARPLEAGTIYRFALRGGSGQLLDTWAFQ
                     AAQPLRIVGTLPETRSADVPLDTGIEVTFDQDGVADAESHFSIRPATNGRFEQHGRTL
                     AFVPDRPLTPSTLFSVTVTRGIKVTDTGEATVVDTRFQFETRAEGVSADNPRTFEFSD
                     AVVESATAERPTIGIWSYGGADKPPTSIGIVVYRFGGIDAAIDAFRTIRARPDWTRWS
                     STGLVDATGLTRVVSADLALNPYRNGFWVQLPDRLPAGWYLVQQSDGSKPIQTVLQVT
                     DVAGYLAVSDTKTLVWANDLHSKAPLVGATVASDGVRFGRTDGHGLAIGTTPRSLLPT
                     TGATCAHPCDPVVVVRTTDGRRVFLPAAAGRDKLEGYGDIYWWSETDARTWSLLHTDR
                     SRYRSGDTVHVWGFARDRDSGAAPATITIRLYGESGDGTDTGAPAVATLVRHPDAAGA
                     INGSLPLAGLPDAYYTLTMSIGSRVIGTTSFAVGPIAKPAYQLDVTTGRRVYIAGDRV
                     KVTVDARFFEGTPVPGVPLRIDGFATDGTFGNATTDALGTAVWRTIAAIDPEQGNEGP
                     NVTTIDAIPARAEEAEITAGSRDVVVFPSSRTIDAVGRIANGKVIVTGGVHVVALNRL
                     EAAIAAGSSIWDLDARGAAVRGATVTVSFIELIPKRHQVGTEYDFVEKKVVPVYETEI
                     IEHTAGTVRVTTAANGSWTASVPASVADHEYRIVATVGDPDGHVARITSYASRQVGSP
                     YETNPLASLAPTTPTANPDDQFSVGERIDLKLTDPDRPQTVGDGSRYLFFTAQRGIHT
                     AVVQASRRYVTTFPAWGPPNLSIGGVRFTGDGYVGTAWFGAGFHAADRQLQVDLSTVA
                     DRYAPGGTVKVNVRTRSASGAPVAATVILRAVDEKLFAIGAAAAEDPLNELYSGIGSG
                     MVGTYRSHRDPQGQPEGGDTTGGGGDDRDDFRDSLLFEAITTGADGRGSTTFKLSDDL
                     TSWRVTAAAVTRRLQAGVGSVLVPVGLPFFVDASIAPEYLLADRPTIAVRTFGSAVAA
                     GTPVTIRVSSASLGFDSGPLPAKAFVNIDVPLPALRSGTQTLTITATTGAGATARTDR
                     LTRTFSVVATRLTRQRTNYVELPSSAAFVGGDGFTSVVIADASGGRYISLLSDLAAGG
                     GARLDRALAADLASKLLKDRFGSEPTDGSVEPFAPGRYQGEDDGLAIVPYGSSDLELS
                     AMVAIVAPDRVDRAKLAYYLRTIRENTTETRERRTYALAGLAGLGDPVLRDVRAAIAD
                     PDLTIREQLMLGLGAASLGDSAIARTILRTVAAAVGEQSGTLARLRVGSSAADVTAGT
                     ALAAVLAAAVGDPLAARFWAYVEANPAADRIEVLPAIAFVTHTLDRLPVQPAKVAWTV
                     DGTRHVVDLATGGSIRLSLTAAQLASLSIDRLAGTVGVTTLWREAVQPAAFHPDPDLT
                     ITRSIQPADPVHASDLVVVDLHVTFNTSAANGCRQVTELVPSGLAPVGAWSHWTDPDA
                     EVDPATADVILPYD"
     gene            complement(5229..>5571)
                     /locus_tag="VHM48_01870"
     CDS             complement(5229..>5571)
                     /locus_tag="VHM48_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754178.1"
                     /translation="EKYEIATRQDHYAFDRDIVLEAPDGTFAAFAMCWADPAGSIGEF
                     EPVGTHPDHQRRGLGRVIMRHGLRLMRAAGLRDALVFSLRSNPASEALYRSAGFHEVA
                     LHRRWTKPLDV"
BASE COUNT          777 a         1882 c         2115 g          797 t
ORIGIN      
        1 tggtcgtacg gcaggatgac gtcggcggtc gccgggtcca cctcggcatc cggatcggtc
       61 cagtgcgacc acgcaccgac cggcgccagg ccgctcggga cgagctcggt cacctggcgg
      121 cagccgttcg cggcggacgt gttgaacgtg acgtggaggt cgacgacgac gagatcggag
      181 gcgtggaccg ggtcggccgg ctggatcgaa cgcgtgatcg tcaggtccgg atcggggtgg
      241 aaggcggcgg gctggacggc ctcgcgccac agcgtcgtca cgccgacggt gccggcgagc
      301 cggtcgatcg acagcgacgc gagttgagct gccgtcaggc tgaggcggat cgatccgccc
      361 gtggcaaggt cgacgacgtg gcgcgtcccg tcgaccgtcc aggcgacctt cgccggctgg
      421 acgggcaggc ggtcgagggt gtgggtcacg aacgcgatgg cgggaaggac ctcgatccgg
      481 tcggccgccg ggttggcctc aacgtacgcc cagaagcgcg cggccaacgg atcgccgacc
      541 gcggcggcga gcaccgccgc gagggccgtc ccggccgtga cgtcggccgc cgacgacccg
      601 acgcgcagcc gggcgagcgt gcccgactgc tcgcccacgg ccgcggcgac ggtgcggagg
      661 atggtgcgcg cgatcgcgga atcgccgagg ctggccgcac ccaggccgag catcagctgc
      721 tcccggatcg tcagatcggg atccgcgatc gctgcccgga cgtcccggag caccgggtcg
      781 ccgagcccgg cgagcccggc gagggcgtac gtgcgtcgct cccgcgtttc cgtcgtgttc
      841 tcgcggatgg tccggaggta gtaggcgagc ttcgcccgat cgacccggtc cggcgcgacg
      901 atcgccacca tcgccgacag ctcgaggtcg ctgcttccat acgggacgat cgcgaggccg
      961 tcgtcctcgc cctggtaccg cccgggcgcg aacggctcga ccgaaccgtc ggtcggctcc
     1021 gacccgaagc gatccttcag cagcttcgac gcgagatcgg ctgccagggc gcggtcgagc
     1081 cgggcgccgc cgccggcggc gaggtcggac agcagcgaga tgtagcgccc gccgctcgca
     1141 tccgcgatca ccacgctcgt gaagccgtcg ccgccgacga aagcggcgct cgacggcagc
     1201 tcgacgtagt tcgtccgctg ccgggtgagg cgggtggcca cgacactgaa tgtccgggtg
     1261 aggcggtccg tccgggccgt cgcgccggcg ccggtcgtgg ccgtgatcgt cagggtctgg
     1321 gtcccgctgc ggagggccgg cagcgggacg tcgatgttca cgaacgcctt cgctggtagg
     1381 ggcccgctgt cgaagccgag gctcgccgac gagacccgga tggtgacggg ggtccccgcg
     1441 gcgaccgcgg agccgaacgt ccggacggcg atcgtcgggc gatcggccag caggtactcc
     1501 ggggcgatcg aggcgtcgac gaagaacggt agtccgaccg ggaccaggac cgagccgacc
     1561 cccgcctgga ggcggcgggt cacggcggcc gcagtgaccc gccacgaggt caggtcgtcg
     1621 gagagcttga acgtggtgct gcctcggccg tccgcgccgg tcgtgatcgc ctcgaacagc
     1681 agcgagtcgc ggaaatcgtc gcggtcgtcg cctccgccgc cggtcgtatc gccgccctcc
     1741 ggctggccct gcggatcgcg atgcgagcgg tacgtcccga ccatcccgct gccgatgccg
     1801 ctgtacagct cgttcaacgg gtcttcagct gcagccgcgc cgatcgcgaa cagcttttcg
     1861 tcgaccgccc ggaggatcac cgtggcggcg accggggcgc cgctcgccga ccgcgtccgg
     1921 acgttgacct tcaccgtccc gcccggggcg tatcggtcgg caacggtgga gagatcgacc
     1981 tggagctggc gatcagctgc gtggaagccg gcgccgaacc aggcggtccc gacgtacccg
     2041 tcgccggtga agcggacgcc gccgatcgag aggttcggcg gaccccaggc cgggaacgtg
     2101 gtcacgtacc ggcgcgacgc ctggacgacg gcggtgtgga tcccgcgctg ggcggtgaag
     2161 aacagatagc ggctgccgtc gccgaccgtc tgcgggcgat ccgggtcggt cagcttcagg
     2221 tcgatccgct cgccgacgct gaactggtcg tcgggattcg ccgtcggagt ggtcggagcg
     2281 agcgatgcca gcgggttcgt ctcgtacggg ctcccgacct ggcggctggc gtaggacgtg
     2341 atcctggcca cgtggccgtc gggatcgccg accgttgcca cgatccgata ttcgtgatcg
     2401 gcgaccgatg ccgggacgga cgcggtccag gacccgttgg ccgcggtcgt gacgcggacc
     2461 gtgcctgccg tgtgctcgat gatctcggtc tcgtagacgg gaacgacctt cttctcgacg
     2521 aagtcgtact cggtccccac ctggtgccgc ttcgggatga gctcgatgaa cgacaccgtg
     2581 acggtcgcgc cccggactgc ggccccgcgc gcgtcgaggt cccagatcga ggagccggcg
     2641 gcgattgccg cctccaggcg gttcagcgcg acaacgtgga cgccgccggt gacgatgacc
     2701 ttgccgttcg cgatccgacc gaccgcgtcg atcgtccggc tgctcgggaa gacgacgacg
     2761 tcgcgacttc cggcggtgat ctcggcttcc tcggcgcggg cggggatcgc gtcgatcgtc
     2821 gtgacgttcg ggccctcgtt gccctgctcg gggtcgatcg cggcgatcgt tcgccagacc
     2881 gcggtcccga gcgcgtccgt ggtggcattg ccgaacgtgc cgtcggtggc gaatccgtcg
     2941 atgcggagcg gcacgccggg gacgggcgtt ccctcgaaga agcgggcgtc gacggtgacc
     3001 ttcacccgat cgccggcgat gtagacccgc cggcccgtgg tcacgtcgag ctggtacgcg
     3061 ggcttggcga tcgggccgac ggcgaacgac gtcgtcccga tgacccggct gccgatcgac
     3121 atcgtcagcg tgtaatacgc gtcgggcagg ccggccagtg gcagcgagcc gttgatcgcc
     3181 ccggcggcgt cggggtgtcg gaccagggtg gcgaccgcgg gagcgccggt gtcggtgccg
     3241 tcgccggact cgccgtacag ccggatcgtg atcgtcgccg gcgcggcgcc gctgtcgcga
     3301 tcgcgggcga agccccacac gtggaccgtg tccccggagc ggtagcgcga gcgatcggta
     3361 tggagcaggc tccaggtcct ggcgtcggtc tcggaccacc agtagatgtc gccgtacccc
     3421 tcgagcttgt cgcgacccgc cgcggcgggc aggaagacgc gccggccgtc ggtcgtccgg
     3481 acgaccacga ccgggtcgca cggatgggcg cacgtcgcgc cggtcgtcgg gagcaggctc
     3541 ctgggcgtgg tcccgatcgc caggccatgg ccgtcagttc ggccgaaccg gacaccgtcg
     3601 ctggcgaccg tcgcgccgac aagcggtgcc ttcgaatgga ggtcgttcgc ccagacgagg
     3661 gtcttcgtgt cggagaccgc gagatagccg gcgacgtcgg tgacctggag gacggtctgg
     3721 atcggcttgc tgccgtcgga ttgctggacg aggtaccagc cggcggggag tcgatcggga
     3781 agctggaccc agaacccgtt gcggtacggg ttgagggcga ggtccgccga gacgacgcgg
     3841 gtgagtccgg tcgcgtcgac cagtccggtg ctcgaccagc gggtccagtc gggacgcgcc
     3901 cggatcgtcc ggaaggcgtc gatggcggcg tcgatgccgc cgaaccgata gacgacgatg
     3961 ccgatggacg tcggcggctt gtcagcgccg ccgtacgacc agatcccgat cgtggggcgc
     4021 tcggcggtcg cggattcgac gaccgcgtcg gaaaactcga aggtccgcgg attgtcggcg
     4081 gagactccct ccgcccgggt ctcgaactgg aaccgcgtgt cgacgacggt ggcctcgccg
     4141 gtgtcggtca ccttgatccc gcgggtgacg gtgacgctga agagcgtgct gggcgtcagc
     4201 gggcgatcgg ggacgaaggc gagggtccgg ccgtgctgtt cgaagcgccc gttcgtggcc
     4261 ggcctgatcg agaagtgcga ttccgcgtcg gcgacgccgt cctggtcgaa ggtgacctcg
     4321 atcccggtgt cgagcgggac atcggccgac cgcgtctcgg gcagggtgcc gacgatccgg
     4381 agcggctgcg ctgcctggaa cgcccacgtg tcgaggagct ggccggagcc gccacggagc
     4441 gcgaaccgat agatggtgcc tgcctcgaga gggcgggccg gcgcgacttc gacggcatgg
     4501 tcgccggcca ccttgttcac cgtgaacgag aacgccggct cgacggtgag cccggccgcg
     4561 agggtcgatg ccggggtgcc gtccgtgctc tcgagacgga agctcgcgtc gagcgggacc
     4621 acgacgccgc tggcaccacg ggccgtgagg agggcggtcg gtgcgggcga gatcccggtc
     4681 agcggctcgt acccctgcga cggttgggcg ctggccgacg gtccgggtcg accactggcc
     4741 gtgggacccc ccgaaccgac ggcgacgtcg gatgccactg gctggctggc gtcggcaacg
     4801 ccgtcagcga gtcgcccgcc gttccaggcg aacagccccg cgacgagcgc cgtggcgacg
     4861 accgcaacgg tcacgacgac cgcccgttgg cgcagccagg ccggacgccg cggtcccgat
     4921 cgtggcccgc ggcgcgtcgt cgacggcgcg tgcgccgcgg tgcctggcgc agcgctctgg
     4981 acccagccgc ggtcatcgag atccgacggc gcgacgggca ggtcgtccgc ccgttcgtga
     5041 tcgaacatcg tcgggctcct cccaccgatt ggtgggccga gcggccgcgt tgcggaccct
     5101 gggtagtcgc ttctcggacc ggcctgctca gcgatgagac gcgaccggct ccgccgtgtt
     5161 acgggtcatc tggccctgtc ggcgggttga cagacgggtc gtccggccgt catgccggcc
     5221 cgcccggatc aaacgtcgag cggcttcgtc cagcgccgat ggagggcgac ctcgtggaac
     5281 ccggcggagc ggtatagagc ctccgacgcg ggattggagc gcagggagaa gacgagcgcg
     5341 tcgcgaaggc ccgccgcgcg catcaggcgc aggccgtgac gcatgatcac ccgcccgagg
     5401 ccacgccgct gatgatcggg atgggtcccg acgggctcga actcgccgat cgagccggct
     5461 ggatccgccc agcacatcgc gaacgcggcg aacgtgccgt cgggcgcttc gaggacgatg
     5521 tcgcggtcga acgcgtagtg gtcctggcgg gtcgcgatct cgtacttctc c
//
LOCUS       DATAIW010000054         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_179754, whole genome shotgun sequence.
ACCESSION   DATAIW010000054 DATAIW010000000
VERSION     DATAIW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179754"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1291
                     /locus_tag="VHM48_01875"
     CDS             545..1291
                     /locus_tag="VHM48_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754179.1"
                     /translation="MATPPTTTDPRAIRPGAIDPVRRSGRPGRLGAGTFVAAIALVGI
                     VLVFATDQVVPASSDHQAQLRIWLASRAAGIVSLVLLAFQIIVGLVLSHPVNKTTWKL
                     SKRLFPWHENVWLFILGFLAAHIVSILLDPFAGVSLAGAFVPGLSSYRSAPVAIGTLS
                     LYALLLTGVTARYTKLLPAGAWLTIHRVGIVVFVLGWMHGLLAGTDSMALIALYTTLG
                     CAVGGAAAYRYWVTRQGRPTFSTSLPEGSR"
     gene            1288..1752
                     /locus_tag="VHM48_01880"
     CDS             1288..1752
                     /locus_tag="VHM48_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754180.1"
                     /translation="MKVGSRVRRGLVMVGVVGSLLVGAASIRAAAVWTAASAPLAVSP
                     ASAESLAARLADEEARSAALQAQLDELRANTTDLSGALDTAKTRISTDTATAKSMRTR
                     LATAKARLATLEKSLAKARAAAAAAARTSGGGGTAPAPAPAATHESEHETDG"
     gene            1745..2242
                     /locus_tag="VHM48_01885"
     CDS             1745..2242
                     /locus_tag="VHM48_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754181.1"
                     /translation="MAEALRRPIPAIRATPAITRPSVRIGALPTVPTHVGTMLGLSVA
                     GYGMALALVTGLQASSEATINANRAPVAAAIDQIAARHDRLGQSLQDAAGQYGTAAAG
                     YQSVSDSLAGFEDRLAALAKSVTAVDGASRALPASVALPPVVRSVRVTTKAATHATSG
                     GSAAP"
     gene            complement(2341..>2887)
                     /locus_tag="VHM48_01890"
     CDS             complement(2341..>2887)
                     /locus_tag="VHM48_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2754182.1"
                     /translation="LRVGVVGFGAVGSRVAALFGAIGCDVAYWTRHRRLPDEERGAAW
                     LPLAKLAGRSDVLVVNVSLAAETRGLVDSAILAELPRGAIVIDASRGGVVDHVALVGA
                     VETGRLRGAAIDVFEREPLPDDSPLRRSDRILLSSHAAATTSQSMARIFALVSDNVRR
                     AVAGEPVRSVVNGLGPVVVRR"
BASE COUNT          421 a         1090 c          998 g          378 t
ORIGIN      
        1 gcctggcgct gcggccagag gagcgggcgc aggagcggcc gcctgcctca ttccgaacac
       61 gagcccgccg ccaccgccgg cgagcaggcc gacggccagg ccgacggcga cgaagccgga
      121 cgccttgccg agcgtcacgc cgcggatcag acgggtccgg cagccgggac agcgtcgagc
      181 gccgagggcg agcggacggg agcacgacgg acactcgaag atcgtctggt cgatctcacc
      241 gatcgcgatc gacggcgtgg gcgaaatcat cgattccggg gccgggctcg ccttgggcgc
      301 gcggcggcgc gtccggcgag cgacatcgac agtcattcga gcgaggcctc ccgcgcgacc
      361 gaagggtcac gacgtcggcg gcaaggtgcc gtcgagtgga gtgtcgggga cgagagtgcg
      421 gccgggtggc acacgaaggt cccgcgacga tccgtacgat cggcggccgg atcgttgaaa
      481 ccttgtgcgc tcgccatgtc tcaacgagtc agcacctggt ccatccgcct caacggggag
      541 atccatggcg acgccaccca cgaccaccga cccgcgcgcc atcaggcccg gcgccatcga
      601 cccggtccga cgatccggac gaccgggacg cctcggcgcc ggcacgttcg tggctgcgat
      661 cgccctcgtc ggcatcgtcc tcgtcttcgc gacggaccag gtcgtgccgg ccagctccga
      721 tcatcaggcc cagctccgca tctggctggc atcgcgcgcg gccgggatcg tgagcctcgt
      781 tttgttggcg ttccagatca tcgtcgggct ggtcctcagc catccggtca acaagacgac
      841 ctggaagctc tcgaagcgcc tcttcccgtg gcacgagaac gtgtggctgt tcatcctcgg
      901 gttcctcgcc gcccacatcg tcagcatcct gctcgatcct ttcgccggcg tcagcctcgc
      961 gggcgccttc gtccccggtc tgtcgagcta tcgcagcgcg ccggtcgcga tcgggaccct
     1021 gtcgctgtac gccctgctcc tgaccggtgt gaccgcccgc tacacgaagc tcctgccggc
     1081 cggtgcctgg ctgacgatcc atcgggtcgg gatcgtcgtg ttcgtcctcg gctggatgca
     1141 cgggctcctc gccggcaccg actcgatggc gctcatcgcg ctctacacca ccctgggctg
     1201 cgccgtcggc ggcgctgccg cgtatcgcta ctgggtcacc cgccagggtc gcccgacgtt
     1261 ctcgacatcg cttccggagg gttcacgatg aaggtaggaa gccgcgttcg acgcggtctg
     1321 gtcatggtcg gcgtggtcgg cagcttgctg gtcggcgctg catcgatccg ggccgccgcg
     1381 gtctggaccg cggcgtccgc tccgctggcc gtctcaccgg catcagcgga gagcctcgcg
     1441 gcccggctgg ccgacgagga ggcccgctcg gctgcgctcc aggcccagct cgacgagctc
     1501 cgggcgaaca cgaccgacct gagcggagcg ctcgacaccg ccaagacgag gatctcgacc
     1561 gacaccgcca cggccaagtc catgcggacg cggctggcga ccgccaaggc gcgtcttgcg
     1621 accctcgaga agtcgctggc caaggctcgc gccgccgcgg cagcggcggc tcgcacctcg
     1681 ggcggcggcg ggacggcgcc ggcgccggcg ccggccgcga cccacgaaag cgagcacgag
     1741 accgatggct gaggcactcc ggcggcccat ccccgcgatc cgcgcgacgc ccgcgatcac
     1801 ccgaccgtcc gtcaggatcg gcgccctgcc gaccgtaccg acccacgtcg gaacgatgct
     1861 cgggctgtcc gtcgccggat acggcatggc cctcgccctc gtcaccggcc tgcaggcgtc
     1921 gagcgaggcc acgatcaacg cgaaccgcgc acccgtggcg gcggcgatcg accagatcgc
     1981 ggctcgccac gatcgactcg ggcagagcct ccaggacgcc gccggtcagt acggcaccgc
     2041 cgccgccgga taccaatcgg tgtccgacag cctggcgggc ttcgaggatc gcctcgccgc
     2101 gctcgcgaag tcggtaacgg ccgtcgatgg ggcgtccagg gcgctcccgg cctccgttgc
     2161 gctcccgccg gtcgtccggt cggtccgggt caccacgaag gcagcgaccc acgcgacgag
     2221 cggcggatcc gccgcaccct gacgcccgca ccttggccgc ggccccccga ccgcggcatc
     2281 gtgaccgccg cggcacgcgc gccgcggcgg tcacgaacct ccgccagctg gcgcccggga
     2341 tcagcgccga acgacgaccg gcccgaggcc gttgacgacg ctccggacgg gctcaccggc
     2401 gacggctcgc cggacgttgt cgctgacgag ggcgaagatc cgcgccatcg actggctcgt
     2461 cgtggcggcg gcatggctcg acagcaggat ccggtccgac cggcggagtg gcgagtcgtc
     2521 gggcagcggc tcgcgctcga agacgtcgat cgcggctccg cgcagccgcc cggtctcgac
     2581 cgcaccgacg agggccacgt gatcgaccac gccgccccgg gacgcgtcga tcacgatcgc
     2641 cccgcgcggc agttcggcga ggatcgccga atcgacgagg ccgcgcgtct cggcggccag
     2701 cgacacgttg acgacgagca cgtccgagcg accggccagt ttggccagcg gcagccaggc
     2761 ggcaccacgc tcctcgtcag gcaagcgacg gtggcgggtc cagtacgcga cgtcgcagcc
     2821 gatcgccccg aacaacgcgg cgacccggct tccgacggct ccgaacccca cgacgccgac
     2881 ccgcagc
//
LOCUS       DATAIW010000055         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_32072, whole genome shotgun sequence.
ACCESSION   DATAIW010000055 DATAIW010000000
VERSION     DATAIW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32072"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VHM48_01895"
     CDS             complement(<1..1430)
                     /locus_tag="VHM48_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2754183.1"
                     /translation="MTATIGAKPHPIFLAGTWVESDHPLVIANPADPDHPAGSTFHAT
                     EAQYEQAVEAAVKAFEVTRTLPAYERGAILRNISAGIKARREELGRLLCLEAGKPIRD
                     ALVEVDRAAVTFRLGAEEAERMVGETIPLDISVAGKGRMGITRRFPIGPIAGISPFNF
                     PLNLAAHKVAPAIASGNPIVLKPPSKDPLVMLTVAEIIEEAGAPAGSVSVLPMSRELG
                     DRMVSDERFKLLTFTGSPSVGWRMKERAGKKRVVLELGGNAGVIIDKSADLDWAVKRC
                     LVGAFAYAGQVCISVQRMFVHEDIWDTFMPKLIEGAMALKLGDPLDPQTDIGPMVDEN
                     AASRTQRWVDEALAAGGKALLGGRANGTFFPPTILTDVPVTAAVCSNEAFAPLVVAFK
                     FTDIDDAIRRVNDSMFGLQTGVFTNDLGAAWRSFNTLEVGGVIVNDVPTYRSDHMPYG
                     GVKDSGLGREGVRFAIEDMTELRIMVL"
     gene            1507..2331
                     /locus_tag="VHM48_01900"
     CDS             1507..2331
                     /locus_tag="VHM48_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX2754184.1"
                     /translation="MCPSRSPSLRELLRVKPGSKLRLADIDPSATHGRDKQGSATELQ
                     KGLDRLTGLQDRLWAEQKHPVLIVLQGIDAAGKDGSVKHVMSAFNPMGCTVTSFKVPT
                     PIELAHDYLWRVHERVPGKGEIAIFNRSHYEDVLVVRVHDIVPKKVWSKRYDQINAFE
                     ELLASTGTTIVKFFLWIDRDEQKARFQDRLDDRDKRWKFRIGDLAERKLWDSYVAADE
                     EALRRCSTDAAPWYVIPANRNWFRNLALADIIADTLDELNPRYPPAPEDLTSVVLE"
     gene            complement(2396..>2671)
                     /locus_tag="VHM48_01905"
     CDS             complement(2396..>2671)
                     /locus_tag="VHM48_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754185.1"
                     /translation="DEHSLVVGTATGPGAWTRVTSVTETPTTVTITVNALMVQLGPAA
                     AYAVPVEITASLHDPIGDRTVIDGSTRLVVQRTRCLPPAYIAPGCTP"
BASE COUNT          421 a          902 c          919 g          429 t
ORIGIN      
        1 aggaccatga tccggagctc ggtcatgtcc tcgatcgcga agcggacgcc ctcgcggccg
       61 aggcccgagt ccttgacgcc gccgtagggc atgtgatcgc tccggtaggt cgggacgtcg
      121 ttgacgatca ccccgccgac ctcgagcgtg ttgaacgacc gccaggcggc gccgaggtcg
      181 ttcgtgaaga ccccggtctg gagcccgaac atcgagtcgt tgacgcgccg gatcgcatcg
      241 tcgatgtcgg tgaacttgaa ggccacgacg agcggggcga acgcctcgtt cgagcagacc
      301 gcggccgtca ccgggacgtc ggtcaggatc gtcggcggga agaatgtgcc attggccctg
      361 ccgcccagca gcgccttgcc acccgccgcg agcgcctcgt ccacccagcg ctgggtccgc
      421 gacgccgcgt tctcgtcgac catcggtccg atgtcggtct gcggatcgag cggatcgccg
      481 agcttgaggg ccatggcgcc ttcgatcagc ttcggcatga acgtgtccca gatgtcctcg
      541 tggacgaaca tccgctggac gctgatgcag acctggccgg cgtaggcaaa ggcaccgaca
      601 aggcagcgct tgacggccca gtcgaggtcg gcggacttgt cgatgatcac gccggcattg
      661 ccgccgagct cgaggacgac ccgcttcttg ccggctcgct ccttcatccg ccagccgacc
      721 gacgggctgc cggtgaacgt caggagcttg aaccgttcgt cgctgaccat gcggtcgccg
      781 agttcgcgac tcatcggcag gacgctgacc gatccggcgg gagcgccggc ttcctcaatg
      841 atctcggcga ccgtcagcat gacgagcgga tccttggacg gcggcttgag gacgatcgga
      901 ttgcccgacg cgatcgccgg cgcgaccttg tgggcagcga gattgagcgg gaagttgaac
      961 gggctgattc cggcgatcgg gccgatcggg aaccggcggg tgatgcccat ccggcccttc
     1021 ccggcgacgg agatgtcgag cgggatcgtc tcgccgacca tccgctccgc ctcctccgca
     1081 ccgagccgga aggtcacggc cgccctgtcg acttcgacga gggcgtcgcg gatcggcttg
     1141 ccggcctcca gacagagcag ccggcccagc tcctcgcggc gcgccttgat cccggcgctg
     1201 atgttgcgga ggatcgcgcc gcgctcgtag gccggcagcg tccgggtgac ctcgaacgcc
     1261 ttgaccgccg cctcgaccgc ctgctcgtac tgcgcttcgg tcgcgtggaa ggtggagccc
     1321 gcgggatggt cgggatcggc cgggttggcg atcacgaggg gatgatccga ttcgacccag
     1381 gtgccggcga ggaagatcgg atgtggcttt gcgccgatgg tggcggtcat cacggggctc
     1441 ctgcgaacgg gctcggtcat gccattctat ccgtgccccc gacgactcca gcctcgaggt
     1501 cctgcgatgt gcccttcccg atcgccgtcc ctgcgtgagc tcctccgggt gaagccggga
     1561 tcgaagcttc ggctcgccga catcgacccg agcgcgaccc acggccgcga caagcagggc
     1621 tcggcaaccg agctgcagaa gggcctcgat cgcttgaccg gcctgcagga ccgcctctgg
     1681 gccgagcaga agcaccccgt cctgatcgtt ctccagggga tcgacgccgc cggcaaggac
     1741 ggctcggtca agcacgtcat gagcgccttc aacccgatgg gctgcaccgt gacgtccttc
     1801 aaggtcccga ccccgatcga gctggcccac gattacctgt ggcgcgtcca cgagcgggtg
     1861 cccggcaagg gcgagatcgc gatcttcaac cgttcgcact acgaggatgt cctggtcgtc
     1921 cgggtccacg acatcgtccc gaagaaggtg tggtcgaagc gctacgacca gatcaacgcc
     1981 ttcgaggagc tgctcgcctc gaccgggacg acgatcgtca agttcttcct ctggatcgac
     2041 cgcgacgagc agaaggcgcg gttccaggat cggctcgacg accgggacaa gcgctggaag
     2101 ttccggatcg gcgacctcgc cgagcgaaag ctctgggatt cctacgtcgc ggccgacgag
     2161 gaagcccttc gtcgctgctc gaccgacgcc gcgccgtggt acgtcatccc ggccaaccgc
     2221 aactggttcc gcaacctggc cctggcggac atcatcgccg acacgctcga cgagctgaat
     2281 ccgcgctacc cgccggctcc cgaggacctg accagcgtcg tgctcgagta ggcggccggc
     2341 gtcgacctgc ggcgatcccg ggtcgatagc cccgggacgg cgccatcgtc gggagctagg
     2401 gagtgcaccc tggagcgata taggcgggcg gcaggcagcg ggtccgctgg acgacgaggc
     2461 gggtgctccc gtcgatgacg gtccggtctc cgatcgggtc gtgcaggctc gccgtgatct
     2521 cgacagggac ggcgtaagct gctgccgggc cgagctgaac catgagggcg ttgaccgtga
     2581 tcgtgaccgt cgtcggcgtc tcggtcacgc tggtgacccg cgtccaggcc ccggggccgg
     2641 tggcggtccc gacgacgagg gagtgctcgt c
//
LOCUS       DATAIW010000056         9389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_91654, whole genome shotgun sequence.
ACCESSION   DATAIW010000056 DATAIW010000000
VERSION     DATAIW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91654"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHM48_01910"
     CDS             complement(<1..191)
                     /locus_tag="VHM48_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2754186.1"
                     /translation="MRVLVTGGAGYVGSVSVERLVEAGHEVVVLDDLSTGHRESVPGA
                     VSLEVGSYGDADVMARLLER"
     gene            complement(238..639)
                     /locus_tag="VHM48_01915"
     CDS             complement(238..639)
                     /locus_tag="VHM48_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2754187.1"
                     /translation="MIELMLEAERAMGIGLLDNAERLYAQVVAIDPKNSIAVTGLARV
                     ALERGDQRGAYTFARRALALDPDNPMASHLSLRMAEQMRNRGEPLPDDPSSATEPVSP
                     APTSDGAPAAAASSKPAARSGIVGRFLRRRR"
     gene            complement(636..1745)
                     /locus_tag="VHM48_01920"
     CDS             complement(636..1745)
                     /locus_tag="VHM48_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2754188.1"
                     /translation="MSATGPVGVGIIGAGVISDTYIRNLTTFADTAVVAIGDLRPEVA
                     RAKAAEHGLAAAGDVDVVLGHPEVEIVVNLTIPAAHAEVALRAIAAGKHVWNEKPLAL
                     DRSGGRAVIDAAASAGLRVGCAPDTFLGAGLQAARRWIEDGAIGEPLTALALLQSPGP
                     DAWHPNPAFLFQTGGGPLFDIGPYYLTALIQTFGAVSEVAAAASTARARRTIGSGPKA
                     GDVFEVTVPSHVGALIRFAGGQSAQTIFSFDSALPRILLEVTGRDATMILPDPNNFDG
                     DVQIRRRGDAATETVARTKARSTRGTGVLDMARAIRANRPHRADGALAYHVLDVMVAI
                     GDAARSGAVERVESSVTPVEPLPADWDPLAATIER"
     gene            complement(1742..3955)
                     /locus_tag="VHM48_01925"
     CDS             complement(1742..3955)
                     /locus_tag="VHM48_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2754189.1"
                     /translation="MTNEVRGEDATADAMPETISAAIPQPVRELLTTLWHGGHAAYVV
                     GGALRDIAGERPAAEGVRVDWDLATSALPEQTVGLFENAVYENRFGTVAVRRDWETYE
                     ITTFRSDHDYADFRRPHRVEFGLSLEADLARRDFTMNAVAWGANAAVEGEPHAPAPGI
                     VDPFGGLADIAAGRIRAVGEPRKRFEEDALRIIRAIRFAATLGWTIEPETLAAIHVTA
                     PLVAHLSGERIAMELEKLLAADRPSVGLRLLADTGVLDAISPDLAAQRGVAQNKITGE
                     DLWDHTLRTVDASANQTIVRLAALVHDIGKPATAADGHFYRHEAVGADLADALLDRLR
                     VPTTTRTRVVHLVRQHMFRYEPSWGDSAIRRFLAKIGPDAIDQLFALREADNAGSGVP
                     RDADDLAGFRARIATELAAGPILDRSALAVDGSDLMAELGVPAGPEVGRILSELFDQI
                     VENPRLNDHDQLMEIARANTPGSQPSSEPAAAEPMTAAASAGELSVQLYAVRASLAQD
                     VDATLERLAALGFRRVEPFDLVAFRERLGKGFRRLGLAAPTAHVGLFGADLEAVFDAA
                     VELGIATLIQPWTDPARWQSDAGVHAVAAELNAVAARAASRGLQVGYHNHHFELASMI
                     GGRHALEAFADLLDPSVALEVDTYWAHVGGADVPALLRRLDGRVVALHVKDGDGSLDN
                     HKQVAVGAGTLPIREIVGAAPAALRVIELDDTAGDMFDALRDSRAFLLALDGSPA"
     gene            complement(4020..4535)
                     /locus_tag="VHM48_01930"
     CDS             complement(4020..4535)
                     /locus_tag="VHM48_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2754190.1"
                     /translation="MNGVVLVLNQNYEPLNVCNLPRAFRLVFGEKAEVIEYDHQVIRT
                     PRTEFHAPSVIRLQYQIRRPRPRVKLSRREIFARDRHTCQYCGRQGHDLTLDHIMPRH
                     RGGGHTWENLVTACKSCNHRKGGKTIEEARFRLTRPPFEPRSDVYSMFTPYLVDEHNE
                     AWRTYLFLGRN"
     gene            complement(4602..5555)
                     /locus_tag="VHM48_01935"
     CDS             complement(4602..5555)
                     /locus_tag="VHM48_01935"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2754191.1"
                     /translation="MSGPTPGPAPADRVRIYEVGPRDGLQNEARPIPLATKLRYLELL
                     ADAGLAEIEATSFVAPNAIPQLADADELMAALPRRAGVRYPVLVPNVRGLARAEAAGV
                     DAVAVFTAASDAFTTRNIRMTVAESLDAFAPVLARAGELGWWRRGYVSTAFGCPYSGA
                     VDPARAVGVALRLLELGVDEVCFGDTIGVGVPPQVGDLTARALDAGIPVANIAYHFHD
                     TRGTALANVAAAMSAGVRCFDASTGGTGGCPYAPGAAGNLATEDLVYLLDAIGLEHGV
                     SLEAVLEAARFIATALDKPLATKVGQAGGWDPRTGQPTGRG"
     gene            complement(5583..5888)
                     /locus_tag="VHM48_01940"
     CDS             complement(5583..5888)
                     /locus_tag="VHM48_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754192.1"
                     /translation="MARDKKKDRDHAKAAKAPGERGTADAAASQHHAAKAHGRATQVD
                     TSLPGTRPELMELHAAARARRNAAALDSPAFRAAVMDLERIEIRIAAIDRAAEPPLG"
     gene            complement(5939..7798)
                     /locus_tag="VHM48_01945"
     CDS             complement(5939..7798)
                     /locus_tag="VHM48_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2754193.1"
                     /translation="MATGDGPSEATPSNTVIRRLFVANRGEIANRITRTCDRLGIVAI
                     VPPTDGPDGVDLLDVDAVIAAARAAAADAVHPGFGFLAENADFAEAVAAAGIGWIGPP
                     PAAIRAMGDKAAARQLARRLGVPTIDGYDGADQTDVALTKAAKRIGYPILVKPAAGGG
                     GKGMRTVRVAADLPAQLAAARREALAAFGDDRLVLERLLEGPRHVEVQVLFDGDGHGV
                     HLGERDCSLQRRHQKVLEESPSPAVRAATRRRLTDAALALAGEVGYVSAGTCEFLLDD
                     LGAVFFLEMNTRLQVEHPVTELVTGRDLVADQIRIAAGEPLGFAQADVRPSGHAIEVR
                     LYAEDAEAGFLPATGPILEVRWPAGDGIRVDSGIAAGDEVTGRFDPMLAKVIAHGADR
                     REALARLTAALDETVVLGVTTNLRFLRWLVRRPEVTRGEARIDTLDAIWRPDRDGATP
                     GHGAPGIPDAIWTEAARLLDAGGWRLNGPARARLVADDGTERSVALGSASADLPIASA
                     RVGDAVHLDVDGRSVAFRVAPPPDVDRAARAAAAHAHGGGPTDVAAPMPGAVLAVHAA
                     VGDAVSAGDPIVTLEAMKMEHVVVAPRAGHVGELGVRTGDQVTRGQSVATIED"
     gene            complement(8004..8576)
                     /locus_tag="VHM48_01950"
     CDS             complement(8004..8576)
                     /locus_tag="VHM48_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX2754194.1"
                     /translation="MDAFLLSFGVIFLAELGDKSQLMALAFATRFPALKVLVAISIAT
                     LIVHLGSVALGATVALALPTNLIAIVAGVAFLVFAAWTLRGDELGEGDEARARRDAAG
                     SVVLTVGTAFFRAELGDKTMLATVTLATNHGAIETWLGSTAGMVAADAIAIAIGKALG
                     TRLPERAIRIGAAVAFVVFGLILIWQGVRS"
     gene            complement(8578..9093)
                     /locus_tag="VHM48_01955"
     CDS             complement(8578..9093)
                     /locus_tag="VHM48_01955"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX2754195.1"
                     /translation="MTDLVACVGCRGLVPDEDGPIHRYMTASPGCWRIYTELGAGAMP
                     RTARSSLTVDAYAVTHPGVAGPQSTPSVWVHLVTLCAVIEKGWGAEHAVRLRRVGADA
                     FDRWPWLDRPESMGAVTVVDVAHAVEAGDGAHADELLEAWIDAAWGAWATHHGAVRAR
                     TDELVARFFEG"
     gene            complement(9090..>9389)
                     /locus_tag="VHM48_01960"
     CDS             complement(9090..>9389)
                     /locus_tag="VHM48_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754196.1"
                     /translation="RATWDAADGETRDATRFEQIVASLPEAERRWQRLALAWMFASDA
                     LRRGRPWRADFVRTVRDLGPWHTTARGWFVIVTQQFTFAIVLAFVWVLLPVFFRL"
BASE COUNT         1377 a         3284 c         3435 g         1293 t
ORIGIN      
        1 cgctcgagga ggcgggccat gacgtcggca tcgccgtagc ttccgacctc gagcgacacg
       61 gcacccggca cggactcgcg gtggccggtg ctcagatcgt cgaggacgac gacctcgtgg
      121 ccggcctcga ccaggcgctc gaccgagacc gagccgacat agccggctcc gccggtcacc
      181 aggacgcgca tcgagacctc cggaactgga cgaggaacgg gccgctggac ggggccatca
      241 tcggcgacgg cggaggaacc ggccgacgat gccggaacga gccgccggtt tcgatgaagc
      301 cgccgccgcc ggagccccgt cggaggtggg cgccggagac acgggttcgg tggccgatga
      361 cggatcgtca ggcagcggct caccgcgatt gcgcatctgc tcggccatgc gcagagagag
      421 atggctcgcc atcgggttgt ccgggtcgag cgcgagcgcc cgtcgggcga aggtgtaggc
      481 cccccgctgg tcgccacgct cgagggcgac gcgggcgagc ccggtgaccg cgatcgagtt
      541 cttcggatcg atggcgacga cctgggcgta gaggcgctcg gcgttgtcga gcaggccgat
      601 gcccatcgcc cgctccgcct ccagcatcag ctcgatcatc gctcgatcgt cgcggccagc
      661 gggtcccagt cggcgggcag tggctcgacg ggagtcacgg agctctcgac ccgctcgacg
      721 gctccggatc gggccgcgtc gccgatggcc accatgacgt cgaggacgtg gtaggcaagg
      781 gcgccgtccg cccgatgcgg ccggttcgcg cggatcgcgc gggccatgtc gaggacgccg
      841 gtcccgcgcg tggagcgggc cttcgtccgc gcgaccgtct cggtcgcggc gtcgccacga
      901 cgacggatct ggacgtcgcc gtcgaagttg ttcgggtcgg gcaggatcat cgtcgcgtcg
      961 cgaccggtga cctcgaggag gatccggggc agggccgagt cgaacgagaa gatcgtctgc
     1021 gcggattggc cgccggcgaa ccggatcaag gccccgacat gactcggaac ggtcacctcg
     1081 aagacgtcgc ccgccttcgg ccccgatccg atcgtccgtc gcgcgcgtgc cgtcgaggcg
     1141 gccgccgcga cctcgctgac cgcaccgaac gtctggatga gcgcggtcag gtagtacggc
     1201 ccgatgtcga acagcggtcc gccgccggtc tggaagagga acgccgggtt cggatgccag
     1261 gcatcggggc ccggcgactg gagcagggcg agggcggtga gcggctcgcc gatcgccccg
     1321 tcctcgatcc atcgacgagc ggcctggaga cccgctccga ggaacgtgtc cggcgcacac
     1381 ccgacgcgca ggccggccga agcggccgca tcgatgaccg ctcggccacc gctccgatcg
     1441 agcgcgaggg gcttctcgtt ccagacgtgc ttgcccgcgg cgatggcccg cagcgccacc
     1501 tcggcgtggg ctgccgggat cgtcaggttg acgacgatct cgacctcggg atgcccgaga
     1561 acgacatcga cgtcgccggc tgccgccagg ccatgctcag ccgccttcgc ccgggcgacc
     1621 tccggccgaa ggtcgccgat ggcgacgacc gcggtgtcgg caaaggtcgt caggttccgg
     1681 atgtaggtgt cgctgatgac cccggcgccg atgatcccga ccccgaccgg gcccgtcgcg
     1741 ctcacgcggg cgacccatcg agcgcgagca ggaatgcccg gctgtcgcgc agggcatcga
     1801 acatgtcgcc ggcggtgtcg tcgagctcga tcacgcggag ggcggccggg gcggccccga
     1861 cgatctcgcg gatcggcaac gttccggcgc cgaccgccac ctgcttgtgg ttgtccaggc
     1921 tgccgtcgcc gtccttgacg tggagggcga cgacccgccc atcgagacgt cggagcaggg
     1981 ccggaacgtc cgctccgccg acatgggccc agtacgtgtc gacttcgagg gcgacgctcg
     2041 ggtcgagcag gtcggcgaag gcttcgagcg cgtggcggcc gccgatcatc gacgccagct
     2101 cgaagtgatg attgtggtag ccgacctgga ggccgcgact cgcggcgcgc gcggcgaccg
     2161 cgttgagctc ggccgcgacg gcgtggacgc cggcgtccga ttgccaacgg gcgggatcgg
     2221 tccagggctg gatcaaggtg gcgatgccga gttcgacggc ggcgtcgaag acggcttcga
     2281 ggtcggctcc gaacaacccc acgtgagcgg tcggcgcggc aagcccgagg cggcgaaacc
     2341 ctttgccgag gcgctcccga aaggccacga gatcgaatgg ctcgacccgc cggaatccca
     2401 gggcggcaag ccgctccagc gtcgcgtcga cgtcctgcgc cagcgatgcg cgaacggcat
     2461 agagctggac cgacagttca ccggccgaag ctgccgccgt catcggttcg gccgccgcgg
     2521 gctcgctcga tggctgggat cctggcgtgt tggcacgggc gatctccatc agctggtcat
     2581 gatcgttcag gcgtggattc tcgacgatct ggtcgaacag ctccgaaagg atgcgcccca
     2641 cctccggacc ggccggcaca ccgagctcgg ccatgaggtc cgaaccgtcg acagcgaggg
     2701 ccgatcggtc gaggatggga ccggcggcga gctccgtggc gatccgggcg cgaaacccgg
     2761 ctaggtcgtc ggcatcgcgc ggcacgccgc tcccggcgtt gtcggcctcg cggagggcga
     2821 acagctggtc gatcgcgtcg ggcccgatct tcgcgaggaa gcgccggatc gccgaatcgc
     2881 cccagctcgg ctcgtagcgg aacatgtgct ggcggacgag gtggacgacc cgggtccgag
     2941 tcgtcgtcgg cacgcggagg cgatcgagga gcgcatcggc gaggtccgcc ccgacggcct
     3001 catgtcgata gaagtgcccg tccgcggccg tggccggctt gccgatgtcg tggacgaggg
     3061 cagccaaacg aacgatcgtt tggttggcgg acgcgtcgac ggtgcggagc gtgtgatccc
     3121 agaggtcctc accggtgatc ttgttctggg ccacgccccg ttgagccgcc agatccggcg
     3181 agatggcatc gagcacgccg gtgtcggcaa gcaaccggag cccgaccgag gggcggtcgg
     3241 ccgccagcag cttctcgagc tccatcgcga tccgctcgcc cgagaggtgg gcgaccagcg
     3301 gcgccgtcac gtggatcgcc gccagcgttt ccggttcgat cgtccagccg agcgtcgcgg
     3361 cgaaccggat cgcccggatg atccggaggg cgtcttcctc gaatcgcttc cgtggctcgc
     3421 cgaccgcccg gatccgcccg gccgcgatgt cggccaggcc cccgaacgga tcgacgatgc
     3481 ccggagctgg tgcgtgcggc tcaccctcga cggcggcgtt ggcgccccag gcaacagcgt
     3541 tcatcgtgaa gtcgcgccgg gccaggtcgg cctcgagcga cagcccgaac tcgacccggt
     3601 gcggacgccg gaagtcggcg tagtcgtggt cgctccggaa cgtcgtgatc tcgtacgtct
     3661 cccaatcgcg ccgaaccgcc accgtcccga agcggttctc gtagaccgcg ttctcgaaca
     3721 gccccaccgt ctgctcgggc agggcggagg tcgcgaggtc ccagtcgacg cggacgccct
     3781 cggcagccgg ccgctcaccg gcgatgtcgc gcagcgcccc accgacgacg tacgccgcgt
     3841 gcccgccgtg ccagagcgtg gtgaggagct cgcggaccgg ctgggggatc gcggcggaga
     3901 tcgtctcggg catcgcgtca gccgtggcgt cttcgccgcg gacctcgttc gtcatgtgct
     3961 catggtcgtc gatggtcggc gcgcggatcg gccgcgccgc ggacagcgag acccggcgct
     4021 cagttgcggc ccaggaagag gtacgtccgc caggcctcgt tgtgctcgtc gacgaggtac
     4081 ggcgtgaaca tcgagtagac gtcgctgcgc ggctcgaatg gcggccgggt cagccggaac
     4141 cgcgcctcct cgatcgtctt gccgcccttg cggtgattgc acgacttgca cgcggtgacg
     4201 aggttctccc acgtgtgccc gccgccgcga tggcggggca tgatgtggtc gagggtcagg
     4261 tcgtggccct gccggccgca gtactggcag gtatggcggt cgcgggcgaa gatctcgcgg
     4321 cggctcagct tgacccgcgg ccgcggccgc cggatctggt actggaggcg gatcaccgac
     4381 ggcgcgtgga actcggtccg tggcgtccgg atgacctggt ggtcgtactc gatcacctcg
     4441 gccttctcgc cgaagacgag ccggaacgcc cgcggcagat tgcagacatt cagcggctcg
     4501 tagttctggt tcaggacgag cacgactccg ttcatcgtgg gcgcacgata ccaagaacgg
     4561 cgcggtgtgc tccggcgttc ttccacatcg atcgtcccgg atcacccgcg accggtcggc
     4621 tggccggttc gcggatccca gccgccggcc tggccgacct tcgtcgcgag cggcttgtcg
     4681 agggcggtgg cgatgaatcg ggcggcctcg aggaccgcct cgagcgagac gccgtgctcg
     4741 agcccgatgg catcgaggag gtagacgagg tcctcggtcg cgagattgcc ggcggctccc
     4801 ggcgcatacg gacagccacc ggtcccgccc gtcgaagcat cgaagcagcg aacgccggcc
     4861 gacatcgctg cggcgacatt cgcgagcgcc gtcccccgtg tgtcgtggaa gtgatacgcg
     4921 atgttcgcga ccgggatgcc ggcgtccagg gcccgggcgg tcaggtcgcc gacctgcggc
     4981 gggacgccga ccccgatcgt gtcgccgaag cagacctcgt cgacaccgag ctcgagcagg
     5041 cgcagggcga cgccgaccgc gcgggccgga tcgaccgcgc cggaatacgg gcagccgaac
     5101 gccgtcgaca cgtagccgcg ccgccaccag ccgagctcgc ccgcccgggc gaggaccggc
     5161 gcgaaggcat cgagcgactc ggcgaccgtc atccggatgt tgcgggtcgt gaacgcgtca
     5221 ctggcggcgg tgaagaccgc gacggcgtcg accccggcgg cctcggcccg ggcgaggccg
     5281 cgaacattcg ggacgaggac gggatagcgg acgcccgctc ggcgggggag ggccgccatg
     5341 agctcgtccg catcggcgag ctgggggatc gcgttcgggg cgacgaagga ggtggcttcg
     5401 atctccgcga gaccggcgtc tgcgaggagc tcgagatacc ggagcttggt cgcgagcggg
     5461 atcggccggg cctcgttctg gagcccgtcg cgcggcccga cctcgtagat ccggacgcga
     5521 tcggccgggg caggtccggg cgtaggtccg ctcaccgggt cgcgccggtc acgctggccc
     5581 gctcagccga gcggcggttc ggccgcccga tcgatcgccg cgatccggat ctcgatccgt
     5641 tcgaggtcca tgaccgcggc ccggaacgcc ggactgtcga gcgccgccgc attccgccgg
     5701 gcccgcgccg cggcatggag ctccatcagc tccggccggg tgccggggag gctggtgtcg
     5761 acctgggtcg cccgaccgtg ggccttcgcc gcgtggtgct ggctcgcggc ggcgtccgcc
     5821 gttccgcgct cgccgggcgc cttcgcggcc ttcgcgtgat cgcggtcctt cttcttgtcc
     5881 ctggccatcg gtcctcctgt gcggagaggg tagcgcgtcc gggcggcccc ttcgcgcgtc
     5941 aatcctcgat cgtcgcgacc gattgcccgc gcgtgacctg gtcgccggtc cggacaccga
     6001 gctcgccgac gtgaccggca cgcggtgcga cgacgacgtg ctccatcttc atcgcctcga
     6061 gggtcacgat cgggtcgccg gcggacacgg catcgccgac ggccgcgtga acggcgagga
     6121 cggcgccggg catcggggcg gcgacgtcgg tcggccctcc gccgtgggcg tgcgccgccg
     6181 cggcccgggc ggcccggtcg acgtccggcg gcggcgcgac ccggaaggcc accgacctcc
     6241 catcgacatc gagatggacc gcgtcgccga cgcgggcgga ggcgatcggc aaatcggcgg
     6301 aagccgaacc cagtgcgacc gatcgttccg tgccgtcgtc ggcgacgagc cgggctcgag
     6361 ccggcccatt gaggcgccac ccaccggcgt cgagcaggcg agcggcctcg gtccagatgg
     6421 cgtcggggat gccgggcgcg ccgtggccgg gcgttgcgcc gtcccggtcc gggcgccaga
     6481 tcgcgtcgag cgtgtcgatc cttgcctcgc cgcgagtcac ctccggacgt cggacgagcc
     6541 agcgcaggaa gcgcaggttc gtggtcacgc cgagcacgac ggtctcgtcg agggcggcgg
     6601 tcaggcgggc cagcgcctcg cgccggtcgg caccatgcgc gatgaccttg gcgagcatcg
     6661 ggtcgaagcg accggtcacc tcgtcgccgg cggcgatccc ggaatcgacc cggataccgt
     6721 caccggccgg ccagcggacc tcgaggatcg ggcccgtcgc cggcaggaag ccggcctcgg
     6781 catcctcggc gtagaggcga acctcgatcg cgtgaccgct cggccggaca tcggcctggg
     6841 cgaagccgag cggctcgccg gcggcgatcc ggatctggtc ggcgacgagg tcgcggccgg
     6901 tcaccagctc cgtcaccggg tgttcgacct gtagacgggt gttcatctcc aggaagaaga
     6961 cggcgcccag gtcgtcgagc aggaattcgc acgtgcccgc gctgacgtag ccgacctcgc
     7021 cggcgagggc gagcgcagcg tcggtcaggc ggcgtcgggt cgcggcccgg acggccgggg
     7081 atggcgattc ctcgaggacc ttctggtggc gacgctggag cgagcagtcg cgctcaccga
     7141 gatggacacc atggccgtcg ccgtcgaaca ggacctggac ttcgacatgg cgggggcctt
     7201 cgaggagccg ttcgagcaca agccggtcgt cgccgaaggc cgcgagcgcc tcgcgcctgg
     7261 ctgctgcgag ctgggcgggc aggtcggcgg cgacgcggac tgtccgcatc cccttgccac
     7321 cgccgccggc cgccggcttg acgaggatcg ggtagccgat tcgcttcgcc gccttcgtca
     7381 gcgcgacgtc ggtctggtcg gcgccgtcgt agccgtcgat cgtcgggacc ccgagtcggc
     7441 gggccagctg ccgggcggcc gccttgtcgc ccatcgcccg gatcgcagcc ggcggcggtc
     7501 cgatccagcc gatcccggcg gcggcgaccg cttcggcgaa gtcggcgttc tcggcgagga
     7561 acccaaaccc ggggtggacg gcgtcggccg cggcggcccg tgcggccgcg atgaccgcgt
     7621 cgacatcgag caggtcgacg ccatcggggc cgtcggtcgg cggaacgatc gcgacgatcc
     7681 cgagccggtc gcaggtccgg gtgatccggt tcgcgatctc gccgcggttc gcgacgaaca
     7741 gccggcggat gaccgtgttt gagggtgttg cctcgctcgg tccatcgcct gtcgccatga
     7801 acgcgtccaa cgcgtcaaac gcgacacgcc agcgccctcc gacacgccgc gcgggcaggc
     7861 gaccggatgc gatccaccgt tggaccgatc gtgtggtggc accgatccgc gcggccgcct
     7921 gactgggact cagttcgtcg gtcatgtctg tcgcgattgt cgcgatcgag gcccgcgatc
     7981 gcaagcgccg atcaagatcg cgctcagctg cggacgccct gccagatcag gatcaagcca
     8041 aacacgacaa acgcgacagc cgcgccgatc cggatcgcgc gttccggcaa ccgcgtcccg
     8101 agcgccttgc cgatcgcgat cgcgatcgcg tcggcagcga ccatccccgc cgtcgacccc
     8161 agccaggtct cgatggcccc gtggttggtc gcgagggtca cggtcgcgag catcgtcttg
     8221 tcgccgagct cggcgcggaa gaaggccgtc ccgaccgtga ggacgaccga gccggccgcg
     8281 tcccgccgcg cccgcgcctc gtcgccctca ccgagctcgt caccgcggag tgtccaggcg
     8341 gcgaacacga gaaacgcgac acccgcaacg atcgcgatca ggttggtcgg gagggccagc
     8401 gcgacggtgg ccccgagcgc caccgaaccg agatggacga tgagcgtcgc gatcgagatc
     8461 gcgaccagga ccttcagcgc cgggaaccgg gtcgcgaagg cgagggccat gagctggctc
     8521 ttgtcgccga gctcggcgag gaagatcacg ccgaacgaca ggaggaaggc gtccatgtca
     8581 gccctcgaag aaacgggcca cgagctcgtc ggttcgggcc cggaccgcgc catggtgggt
     8641 cgcccaggcg ccccatgccg cgtcgatcca ggcctcgagg agctcgtccg cgtgtgcacc
     8701 atcgccagcc tcgaccgcgt gagcgacgtc gacgacggtc accgcgccca tcgactccgg
     8761 ccggtcgagc catggccagc gatcgaacgc gtcggccccg acgcgtcgca accggacggc
     8821 gtgttcggct ccccatccct tctcgatcac cgcgcagagc gtcacgaggt ggacccagac
     8881 cgatggcgtc gactgtggac ccgccacgcc gggatgggtg accgcatacg cgtcgacggt
     8941 cagcgacgag cgcgcggttc gcggcatcgc ccccgcaccc agctccgtgt agatccgcca
     9001 gcaacccggc gacgccgtca tgtagcgatg gatcggcccg tcctcgtcgg gcacgagccc
     9061 gcggcacccg acgcatgcga caaggtcggt cacagccgga agaagaccgg gagcagcacc
     9121 catacgaacg cgagaacgat ggcgaaggtg aactgctggg tgacgatgac gaaccagccc
     9181 cgcgcagtcg tgtgccacgg gcccaggtcg cgaacggtcc ggacgaagtc agcgcgccag
     9241 ggtctgccac gccgcagggc atcggacgcg aacatccacg ccaatgcgag acgctgccat
     9301 cgacgttcgg cctcgggaag gctcgcgacg atctgctcga agcgggtcgc atcccgcgtc
     9361 tcgccgtcgg cggcgtccca cgttgcccg
//
LOCUS       DATAIW010000057         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_61901, whole genome shotgun sequence.
ACCESSION   DATAIW010000057 DATAIW010000000
VERSION     DATAIW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61901"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VHM48_01965"
     CDS             complement(<1..934)
                     /locus_tag="VHM48_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2754197.1"
                     /translation="MPTELVPIIIAAAAAGAILLIALGLAGNSPVDPVQARLTQLGTM
                     QAKNLEELELQAPFLERTLRPLAVGLSGRMSRVASSSFQEKAEKRLALAGNPGNFRVA
                     DWLGIKAVAAIVGALLFVFLFGVVGFMALPPVLRLLMGVVGLMFGYTIPEFWLGGRVR
                     KRQHLILLQIPDCLDLLTISVRAGLGFDAALAKVVEKLEGPLVDEFRRALAEVRVGKA
                     RRDALRDIVPRTEVVPLTNFIGAIIQAEQLGVSISKVLQVQSEQLRIERRQRAEEMAA
                     KAPIKMLFPLVGCIFPSLFIIILGPAIILIMQNLK"
     gene            complement(938..1948)
                     /locus_tag="VHM48_01970"
     CDS             complement(938..1948)
                     /locus_tag="VHM48_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2754198.1"
                     /translation="MTLLVAGIAAAAIFLIAVGIATSGGGSGVSARLERYASAREGGK
                     ATTEGSGQGGLAELLEKSVALAQLNRVVEARDFGANLARDIARADLKLKPSEFLAIWG
                     ASIVGIPFLFIVLSLGLPGLRHPLALLIGAFVGFMLPRFWLGRRKSGRLGSFNKQLPD
                     TITLLANGLRAGSSFLQAVELVVRESRPPMSTEFSRVIREVNLGLPFEAAMENMVRRV
                     RSEDLELMATAINIQHTVGGNLAEILDSIAYTIRERIRIKGEIRTLTAQQRLSGYVVG
                     FLPIALAGFLFVAAPNFMQPMFEKPPEALGLPLGVVILAFGGFMMFIGFMLIRKIVDI
                     EV"
     gene            complement(1973..3382)
                     /locus_tag="VHM48_01975"
     CDS             complement(1973..3382)
                     /locus_tag="VHM48_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX2754199.1"
                     /translation="MSLLKRIESARPGAGPAGPSSPGLPAVPPSGAGGTPLATPAQRL
                     MTQAPVRESFRDVKFRIQSRVIQDLDPKLDLSNQVEVRRQIEEIFGKVIDEEGLALTR
                     AERVRMLEQITDEIIGLGPLEPLLRDESISEVMVNGPRQVYIERSGKLELTNVVFQND
                     DHVMRIIDRIIAPIGRRVDESSPMVDARLTDGSRVNAIIPPLSLVGPVITIRKFSASP
                     FTVDDLVRFGTATPDMFDFLRACVEARLNIFVSGGTGSGKTTTLNVLSSFIPNDERIV
                     TIEDAAELQLRQEHVVTLESRPPNIEGKGAIPIRELVRNSLRMRPDRIIVGECRGGEA
                     LDMLQAMNTGHDGSMSTGHANSPRDMLSRLETMVLMAGVDLPVRAIREQIASAVDLIV
                     QQSRLKDGTRKIVNITEVQGMEGDVIVMQDVFVFEQTGIVEGKIQGRLKATGIRPKFV
                     EKFEVMGIHLPPGLFGFSY"
BASE COUNT          583 a         1156 c         1261 g          544 t
ORIGIN      
        1 ccttgaggtt ctgcatgatc aggatgatcg ccgggccgag gatgatgatg aacagcgacg
       61 ggaagatgca gccgacgagc gggaacagca tcttgatcgg cgccttggcc gccatctcct
      121 ccgcccgctg gcggcgctcg atgcggagct gctccgactg gacctggagg accttgctga
      181 tcgacacgcc gagctgctcg gcctggatga tcgcgccgat gaagttcgtc agtgggacga
      241 cctcggtgcg gggcacgatg tcccgcagcg cgtcacgccg cgccttgccg acgcggacct
      301 cggcgagcgc ccggcggaac tcgtcgacga gcgggccctc gagcttctcg acgaccttgg
      361 ccagcgccgc atcgaagccg aggccggccc ggacggagat cgtgagcagg tcgaggcagt
      421 ccgggatctg gagcaggatc aggtgctggc gcttgcggac ccggccaccg agccagaact
      481 cggggatcgt gtagccgaac atcagcccga ccacgcccat gaggaggcgc aggaccggcg
      541 gcagcgccat gaacccgacg acgccgaaca ggaagacgaa caggagcgcg ccgacgatgg
      601 ccgcaacggc cttgatcccg agccagtcgg cgacccggaa gttgcccgga ttgccggcca
      661 gggcgagccg cttctcggcc ttctcctgga acgagctgct cgccacgcgg gacatccgcc
      721 ccgagaggcc gacggccagc ggtcgcaggg tccgctcgag gaacggcgcc tggagctcga
      781 gctcctcgag gttcttggcc tgcatcgtgc cgagctgggt cagccgggcc tggaccgggt
      841 cgacgggcga gttgcccgca agcccgaggg caatgagcag gatggcgccg gccgcggcgg
      901 cggcgatgat gatcgggacg agctcggtgg gcatcgacta gacctcgatg tcgacgatct
      961 tgcggatgag catgaacccg atgaacatca tgaacccgcc gaaggcgagg atcacgacgc
     1021 cgagcggcag cccgagggcc tccggcggct tctcgaacat cggttgcatg aagttggggg
     1081 ccgcgacgaa gaggaagccg gcgagagcga tcggcaggaa gccgacgacg tagcccgaca
     1141 ggcgctgctg ggcggtcagg gtccggatct cgcccttgat ccggattcgc tcccggatcg
     1201 tgtacgcgat cgagtcgagg atctcggcga ggttgccgcc gacggtgtgc tggatgttga
     1261 tcgccgtcgc catcagctcg aggtcctcgg accggacgcg gcggaccatg ttctccatcg
     1321 ccgcctcgaa cggcaggccg aggttgacct cgcggatgac ccggctgaac tccgtcgaca
     1381 tcggcggccg cgactcgcga acgacgagct cgacggcctg gaggaacgag gagcctgcgc
     1441 ggagaccgtt ggccagcagg gtgatcgtgt cgggcagctg cttgttgaac gagccgagcc
     1501 ggccgctctt gcggcgaccc agccagaacc gcggcagcat gaagccgacg aaggcgccga
     1561 tcaggagtgc cagcgggtgg cggaggcccg gcaggccgag cgacaggacg atgaacagga
     1621 acgggatgcc gacgatcgag gctccccaga tggcgaggaa ctccgacggc ttgagcttga
     1681 ggtcggcccg ggcgatgtcg cgcgccaggt tggccccgaa gtctcgcgcc tcgacgaccc
     1741 ggttcagctg ggcaagcgcc acgctcttct cgagcagctc ggcgaggccg ccctgtccgg
     1801 atccttcggt cgtggccttg ccgccctcgc gggccgaggc gtagcgctcg agacgggcgc
     1861 tcaccccgga gcccccgccg gacgtcgcga tcccgaccgc gatcaggaag atcgcggcgg
     1921 cggcgatgcc ggcgacgagc agcgtcattg gcatatcagc tgtcctcccc gatcagtaac
     1981 tgaagccgaa gaggcccggc gggagatgga tgcccatcac ctcgaacttc tcgacgaact
     2041 tggggcggat gccggtcgcc ttgaggcggc cctggatctt gccctcgacg attccggtct
     2101 gctcgaagac gaacacgtcc tgcatgacga tgacgtcgcc ttccatgccc tggacctcgg
     2161 tgatattgac gatcttccgg gtcccgtcct tgagccggct ctgctggacg atcaggtcga
     2221 ccgccgacgc gatctgctcc cggatcgccc ggaccggcag gtcgacgccg gccatcagga
     2281 ccatcgtctc gagccgcgac agcatgtcgc gggggctgtt ggcgtggccg gtcgacatcg
     2341 agccgtcgtg gccggtgttc atcgcctgga gcatgtccag cgcctcgccg ccgcggcact
     2401 ctccgacgat gatccggtcg ggccgcatcc ggagggagtt gcggacgagc tcgcggatcg
     2461 ggatcgcgcc cttgccttcg atgttcggcg ggcgggattc cagggtcacg acgtgctcct
     2521 ggcgaagctg gagctcggcc gcgtcctcga tcgtcacgat ccgctcatcg ttggggatga
     2581 aggacgagag cacgttgagg gtcgtcgtct tgccggagcc ggtgccgccg gagacgaaga
     2641 tgttgagccg cgcctcgacg caggcccgga ggaagtcgaa catgtccggg gtcgccgtcc
     2701 cgaaccggac gaggtcgtcg accgtgaacg ggctggccga gaacttccgg atcgtgatga
     2761 ccggaccgac gagcgacagc ggcgggatga tcgcgttgac gcgggagccg tcggtcaggc
     2821 gggcgtcgac catcgggctc gactcgtcga cgcggcgacc gatcggggca atgatccggt
     2881 cgatgatccg catgacgtgg tcgtcgttct ggaacacgac gttggtcagc tcgagcttgc
     2941 ccgaccgctc gatgtagacc tggcgggggc cgttgaccat gacttccgag atcgactcgt
     3001 cgcgcaggag cggctccagc gggccgaggc cgatgatctc gtcggtgatc tgctcgagca
     3061 tccggacgcg ctcggcgcgg gtcagggcga ggccttcctc gtcgatgacc ttgccgaaga
     3121 tctcctcgat ctggcgccgg acctcgacct ggttcgacag gtcgagcttc gggtcgagat
     3181 cctggatgac ccggctctgg atccggaact tgacgtcgcg gaacgactcc cggaccggcg
     3241 cctgggtcat caggcgctgg gccggcgtcg cgagcggcgt gcccccggcc ccgctcggcg
     3301 gtacggccgg caaccccggc gacgacggtc cggcgggacc ggcccctggt cgagcactct
     3361 cgatccgctt cagcagtgac atgacatcag accccttatc gccccgcgag gctcgctacc
     3421 gacacgatca tcggaatgag aacagagatt tcttctggac cgtcttgcgg gtgtcttcgc
     3481 gtgcgctcgg cacccgctcc ccggcaacgg cctgggccag ccggaggatg tcctgggaga
     3541 cctg
//
LOCUS       DATAIW010000058         4307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_2284, whole genome shotgun sequence.
ACCESSION   DATAIW010000058 DATAIW010000000
VERSION     DATAIW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHM48_01980"
     CDS             complement(<1..188)
                     /locus_tag="VHM48_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2754200.1"
                     /translation="MVTETPLEGRGRHAPAVGRQVRRWRAERGLTLARVAEAAGLNIG
                     YLSQIENDKASPSLACLSA"
     gene            263..961
                     /locus_tag="VHM48_01985"
     CDS             263..961
                     /locus_tag="VHM48_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2754201.1"
                     /translation="MITANSQAECRSRRIPAMQIAIIGAGNVGRALATSLTRAGHDVT
                     ITAEHAEHAAEAASQSGATAGTSNADAAANAQVVVLAVPAQSIGQIAGAMGSSLDGKV
                     VIDVSNRPTPTADGLATSIAEELQGTLPSAHVVKAFNTAFASRQANAQVAGIAPDGFV
                     AGDDVTAKQTVLDVVESIGFRPVDAGSLAAARTLEGMAWLNIQRNLAGGTWQDAWVLV
                     GPETVAAATADSRN"
     gene            992..1483
                     /locus_tag="VHM48_01990"
     CDS             992..1483
                     /locus_tag="VHM48_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754202.1"
                     /translation="MSIPVARLNHAVLFVRDAERAAEFYTRIFGFEVVGKEMGGQAIF
                     LRSPLEGNHHDLGLFSVGPQAQNAPRGSVGLYHLAWEVPTIADLADARDVLAAAGALG
                     GASDHGVSKSLYGRDPDGNEFEVMWRVPREAWGEDEHRGVIRPLDIEHELERFGGGSS
                     APA"
     gene            complement(1630..2373)
                     /locus_tag="VHM48_01995"
     CDS             complement(1630..2373)
                     /locus_tag="VHM48_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2754203.1"
                     /translation="MFSIRSFLGRMFAMPEYPPDERDRRTFRLAGLDLPLRATVVVVA
                     VILIVIFDFQRTLIPDALVQYDRNPGIQRLQALSRVVLFFCVPLLIVLVGFRDRPGRY
                     GLQLGDWRWGLGLAVAGCVVMTPIVLMLSSAPDFKVYYAPSAEPLPGLLTTNLMDLVS
                     TEFIYRGFLMLALARVFGPIGLVVALFPFTFTHLTKPEAELLSTFAGGAVYGWLAWRT
                     RSVLWGALAHVYIVTLIIVAAGGFGPGSS"
     gene            2528..3322
                     /locus_tag="VHM48_02000"
     CDS             2528..3322
                     /locus_tag="VHM48_02000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754204.1"
                     /translation="MDDATLARLEHENMIETFTSGVANAPGSLVRRGDGVALLASGLP
                     IRFFNQILIEDDHATTDAIAAALAALRERGKPFVVHLRRGSDDRFRPLMQQLGLVLPA
                     DETPLPGMALHPIPPARIAVAAGHEIRRATDDAGVEDHLRTGALGFGMPEDMLRAIVP
                     PDTWRRPGTFVYVGYTAGEPVTTGFGVRTGRTIGVYNIATIEAARRQGLGAAMTERVA
                     ADGAAEGCDVAILQASPMGLPIYERLGYRTVVEYDGWIEPDQPTEM"
     gene            3322..4233
                     /locus_tag="VHM48_02005"
     CDS             3322..4233
                     /locus_tag="VHM48_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2754205.1"
                     /translation="MRTLEIGLVLPMEESWTDGATPRWAEIRELALRAEQLGFDTVWI
                     PDELLWRPPDGEVRGWWECVAMSGAVAAATSRVKVGTWILSALHRNPGLTAKAVETLD
                     EISGGRFVFGLGSGHAGRQAHAFGLPEDHVFGRFEEAVEIIVPLLRQARADFDGTFHA
                     ARDLEHRPVGPRPGKIPIMIGAKGPKMLRLAALHADIWSWYVEERSDLEEFGPRLAAL
                     EAACVEVGRDPATIGKSAGIIVEPTSVTGAAEVLGSPVRGSAEEIADAFRAFGAGGFT
                     QIEILLWPRTLAALDAMAPVLELLDAD"
BASE COUNT          674 a         1497 c         1533 g          603 t
ORIGIN      
        1 gccgacaggc aggcgagcga cggagacgcc ttgtcgttct cgatctggga cagatagccg
       61 atgttgagtc cggccgcctc ggcgacccgg gccagggtca ggccgcgttc cgcgcgccag
      121 cggcgaacct ggcgaccgac cgccggcgcg tgtcgtccgc ggccctcgag cggcgtttcg
      181 gtgaccatga gtttgctctc cacaacatga tgttgacagc gacaagtgac cgtcgtatgg
      241 ttgtgctatc aacaaatatc gcttgatcac agccaacagt caagcggaat gcagatcccg
      301 gaggatccca gccatgcaga ttgccatcat cggtgccggc aacgtcggtc gcgccctcgc
      361 cacgtcgctg acccgagcgg gccacgacgt cacgatcacg gccgagcacg ccgagcacgc
      421 cgccgaggcg gccagccagt cgggtgccac cgccggcacg agcaacgcgg acgctgcggc
      481 caacgcccag gtcgtcgtcc ttgcggttcc cgcccagtcg atcggccaga tcgcgggcgc
      541 gatggggtcg agcctcgacg gcaaggtcgt catcgacgtc tcgaaccgcc cgacaccgac
      601 cgccgacggg ctggcgacct cgatcgccga ggagctgcag ggcacgctgc ccagcgccca
      661 cgtcgtcaag gcgttcaaca ccgcctttgc ctcgcgccag gccaacgccc aggtcgccgg
      721 gatcgccccg gacggcttcg tcgccggcga tgacgtcacg gccaagcaga ccgtcctcga
      781 cgtcgtcgag tcgatcgggt tccggccggt ggatgccggc tcgctcgcgg ccgcccggac
      841 cctcgagggg atggcctggc tgaacatcca gcgcaacctc gccggcggca cgtggcagga
      901 tgcatgggtc ctcgtcggtc cggagaccgt cgccgcggca accgccgaca gccgcaactg
      961 agccgccgaa ccccgagttg aagggaaccc gatgagcatt cccgtcgccc gcctgaacca
     1021 cgccgtcctc ttcgtccggg atgccgaacg cgccgccgag ttctacaccc ggatcttcgg
     1081 gttcgaggtc gtcggcaagg agatgggcgg acaggcgatc ttcctgcgca gtccgctcga
     1141 gggtaaccac cacgacctcg gtctcttctc ggtcgggccg caggcgcaga atgcgccgcg
     1201 cggctcggtc gggctgtacc acctcgcctg ggaggtcccg acgatcgccg atctcgccga
     1261 cgcccgggac gtcctcgcgg cggccggcgc actcggcggg gcgagcgacc acggcgtctc
     1321 gaagtcgctc tatggtcgcg atcccgacgg caacgagttc gaggtgatgt ggcgcgtccc
     1381 gcgcgaggcg tggggtgagg acgagcatcg cggggtcatc cggccgctcg acatcgagca
     1441 tgaactcgag cggttcggcg gggggtcgtc cgcccccgcc tgatcagcgg cggtgcctcg
     1501 aggcccgtcg ttcgccggtg attgatctcc caggggatct gtggcggctg aacgagcgtg
     1561 cccgaatcga ccattcggca agttcctgtt ccgcgccgcc cgcgatggat cgcccacagc
     1621 cgggccgccc tacgagctcc cgggaccgaa ccctccggcc gcgacgatga tcagcgtcac
     1681 gatgtagacg tgggccagcg cgccccacag caccgaccgc gtccgccagg ccagccagcc
     1741 gtagaccgca ccgccggcga acgtcgagag gagctcggcc tcgggcttgg tcaggtgggt
     1801 gaacgtgaac gggaagagcg ccacgaccag cccgatcggc ccgaacaccc gggcgagggc
     1861 cagcatcagg aagccgcggt agatgaactc ggtcgagacg aggtccatga ggttggtcgt
     1921 cagcaggccc ggcagcggct cggccgacgg cgcgtagtac accttgaagt cgggcgcgct
     1981 cgacaacatg agcacgatcg gcgtcatcac gacgcagccg gcgacggcaa gcccgagacc
     2041 ccagcgccag tcaccgagct ggagcccgta gcgcccgggc cggtcgcgga acccgacgag
     2101 cacgatcagg agcggcacgc agaagaacag cacgacgcgg ctcagcgcct ggagccgctg
     2161 gatgcccggg ttgcggtcgt actggacgag tgcgtcgggg atcagcgtcc gctggaagtc
     2221 gaagatgacg atcaggatga ccgcgacgac cacgacggtg gcccgcagag gtaggtccag
     2281 tccggccagg cggaacgtcc gccggtcgcg ctcgtcgggc gggtactccg gcatcgcgaa
     2341 catccggccg aggaatgagc gaatgctgaa cacggcggca gcctagcagc gcccgacagg
     2401 tgtggctgac cggctgctac cgtccgcgtc gtggacgacg cgacgttggt ctaccgcggc
     2461 tgatcgctcc aaccgccagc cgcccgatcg tcgcacggcc cacccgtccg gctggtagcg
     2521 tccgcgcgtg gatgacgcga cgctcgcccg cctcgagcac gagaacatga tcgagacgtt
     2581 cacatctggc gtggcgaacg ctccgggcag tctcgtccgg cgcggcgacg gcgtcgcctt
     2641 gctggcgtcc ggcctgccga tccgcttctt caaccagatc ctgatcgagg acgaccatgc
     2701 gacgaccgat gcgatcgctg ccgccctcgc ggcattgcgc gagcgcggca agccattcgt
     2761 ggtccatctc cggcgcgggt ccgacgaccg gttcagaccg ctcatgcagc agctcggcct
     2821 cgtgcttccc gccgacgaga ccccgctgcc ggggatggcg ctccacccga ttccgcccgc
     2881 gcgcatcgcg gtcgcagccg ggcacgagat ccggcgggcc accgacgacg ccggcgtgga
     2941 ggatcacctc cggaccgggg cgctcgggtt cggcatgccg gaagacatgc tgcgagcgat
     3001 cgtcccgccg gatacgtggc ggcgcccggg gacgttcgtg tatgtcggct acaccgccgg
     3061 cgagcccgtg acgacgggct tcggggtccg gaccggccgg acgatcgggg tctacaacat
     3121 cgcgacgatc gaggccgcgc gtcggcaggg gctcggggcg gcgatgaccg agcgggtcgc
     3181 ggctgacggt gcggccgaag gatgcgacgt cgcgatcctc caggcgagcc cgatgggcct
     3241 gccgatctac gagcggctcg ggtaccggac cgtcgtcgag tacgacggct ggatcgagcc
     3301 cgaccagcca acggagatgt gatgcgaacg ctcgagatcg ggctcgtgct tccgatggag
     3361 gagtcctgga ccgacggcgc gaccccgcgc tgggccgaga tccgcgagct cgcgctccgg
     3421 gccgagcagc tcgggttcga cacggtctgg atcccggatg agctgctgtg gcgtccgccc
     3481 gacggagagg tccgcggctg gtgggaatgc gtcgcgatga gcggcgcggt ggcggcagcg
     3541 acctcgcgag tgaaggtcgg cacgtggatc ctgtccgcgc tccaccgcaa ccccgggctc
     3601 accgcgaagg ccgtcgagac gctcgacgag atcagtggcg gccgcttcgt cttcggcctc
     3661 ggctcgggac atgccgggcg ccaggcgcat gcgttcgggt tgccggagga ccacgtcttc
     3721 ggccggttcg aggaggcagt cgagatcatc gttccgctct tgcggcaggc ccgggccgac
     3781 ttcgacggga cgttccacgc ggcgcgcgac ctggagcacc gccccgtcgg accacgcccc
     3841 ggcaagatcc cgatcatgat cggcgccaag ggcccgaaga tgctccgcct cgccgccctc
     3901 cacgccgaca tctggagctg gtacgtcgag gagcggagcg acctcgagga gttcgggccg
     3961 cggctcgccg cgctcgaggc agcctgcgtc gaggtcggtc gcgatccggc gacgatcggg
     4021 aaatcggccg gcatcatcgt ggagccgacg tcggtcacgg gtgcggccga ggtgctcggg
     4081 tcgccggtgc gcggctcggc ggaggagatc gcggatgcgt tccgggcgtt cggcgccggc
     4141 ggcttcacgc agatcgagat tctgctctgg ccacggacgc tagccgcgct cgacgcgatg
     4201 gcgccggtgc tcgagctcct cgacgcggac tgatcccgga tcggtcaggc gccgcccgcg
     4261 agcgcgccga ggtgctcggt caggcggagg ttctcgcggt agtcgat
//
LOCUS       DATAIW010000059         4626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_120276, whole genome shotgun sequence.
ACCESSION   DATAIW010000059 DATAIW010000000
VERSION     DATAIW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120276"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHM48_02010"
     CDS             complement(<1..431)
                     /locus_tag="VHM48_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754206.1"
                     /translation="MTSIRAATAAELADWDERTVDAPGGHVYQSRAWAEHRVAAGWAA
                     DHLVFEDGSAVLALRRLFPLVVGCSAYVPRGPIPRDEEPAVTADRLTTLVEHLRMASA
                     AVVATDAEIPASTGYRELLASVGFRPIEEIQPSRHRVSLPLA"
     gene            complement(428..1639)
                     /locus_tag="VHM48_02015"
     CDS             complement(428..1639)
                     /locus_tag="VHM48_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2754207.1"
                     /translation="MTGATASGTAPRSDDDQPATPTVPPADVAAASTAVIAITANDQS
                     WDAFVAAGNPGSYLQLAGWSRVKAVNGWTRALLARESLAAGAAPVGAAPVGGAPVGAQ
                     ILVRRPRPLPWAFAYAPRGPVTAAWTPDTIGTFTAEVRAALTAAVGRVSHLRIDPEIE
                     RDGPADSDGAIRRSLRAAGWRPASPIQPNATRIIDLAPDEETLWGDLRKKWRQYVNKA
                     RTAGITVTDAEGDALPEFYRVYRETADRAGFLIRTEQAYRDIWEAYRPTGNARLLFAR
                     RPDGEALATLFLVRAGTRVVEPYGGMTAEGGESRANYLLKWEAIRTSRELGATSYDLW
                     GLATGGIAHFKTGFGGREIQYIGAWDLVLDPLGRQAYERAQQARVWWTRRRHGLAGGG
                     SAADFTGGDAT"
     gene            complement(1636..3207)
                     /gene="murJ"
                     /locus_tag="VHM48_02020"
     CDS             complement(1636..3207)
                     /gene="murJ"
                     /locus_tag="VHM48_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2754208.1"
                     /translation="MSSSRALARAGLIVSGAFLVSRILGWVRLVVFAHVFPAGSDLDV
                     FFAAFRLPDLMFQLVAAGALSSAVIPIVSSLLATDETARAWRVVSTIANLMLAGLLAL
                     GLVFLVAAPAIIPAITPKFTEAEWSRTVDLTRIMILSPIFLALGSLVTSVLNARGRFA
                     ASAIAPIVYNLAIIGAAIALTSSFGITGLAIGVVAGSLGHLLIQLAPLRAQGFRYERR
                     IDIADPEARKALKLMAPRALGLGAGQITFIVLTSIASGLGTAAITAFNYAFTLLQIPI
                     GLIGVPLGIVVLPSLSSLAAVGELDAFAGLISRAIRLTIFAMLPVTGIAIVLRHEIVD
                     VLFGYSRFDRAALDLIAATLLTFLLGLTAHALIAILARAFYARQDTRTPVAAAILAVV
                     INSTLAGVLAGPLGLPGLGLAIAIAAWVETVVLLVLLQRSLPELAFRPLLSLGIRSLT
                     VTIGGSLAAAGVLAGLTLAMGENPGRFDLVIRMGLVGLVWLLTSAALALVLRIAELTS
                     IIGLMLELIRRPHRA"
     gene            complement(3204..4187)
                     /locus_tag="VHM48_02025"
     CDS             complement(3204..4187)
                     /locus_tag="VHM48_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX2754209.1"
                     /translation="MTDATAESALDRFLRSLAARDTSAHTQRSYGATLTVYLDWLAGR
                     GVDWRTPSRADLRTYLAVLGMGRARSSTAQRLAAIRSFHRFAVREELAPGDPWGVIAT
                     PRLPRRLPRVLEVDEVERLLAAVDGALEDDAASPSELTVAIAIRDRAIVETAYAAGLR
                     ISELASAELGALDLRRGEVRVLGKGRKERIGLLGRPARQALAGYLEDGRPVLVARRRG
                     HGREDAGPTEIFLNHRGEAIGVRGLRYRLDLLRQRAGLPAGVSPHTLRHSFATHLLEG
                     GADLRVVQELLGHESLATTQVYTHVSPARLRSAYRGAHPRATRQPPEPARS"
     gene            complement(4231..>4626)
                     /locus_tag="VHM48_02030"
     CDS             complement(4231..>4626)
                     /locus_tag="VHM48_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEX2754210.1"
                     /translation="HLIPRRARRTGNVFYGCSNYPKCDYTTNFEPVGALHDTDDGPVA
                     KHGDTGICLRCGAAIPLVEGVDVVGRKLEGGPPNPEALAKPARGGARRGRSAGGSGAK
                     GGGRSGEARPGARGGARRTTRSAGAAPDA"
BASE COUNT          692 a         1582 c         1798 g          554 t
ORIGIN      
        1 gcgagcggca gtgacacccg atggcgtgat ggctggatct cctcgatcgg acggaacccg
       61 accgatgcga gaagctcgcg atacccggtg gacgccggaa tctcggcgtc cgtcgcgacc
      121 acggccgcgg aagccatccg cagatgctcg acgagcgtcg tcaatcgatc ggcggtgacg
      181 gccggctcct cgtcgcgggg gatcggacct cgtggaacgt aggcgctgca gccgaccacg
      241 agcgggaaca gacggcgcag ggcgagcacc gcggagccgt cctcgaacac gagatggtcg
      301 gccgcccagc cggcggccac ccggtgctcg gcccaggccc gggactggta gacgtggccg
      361 cccggcgcgt cgacggtccg ctcgtcccag tcggccagct ccgccgcggt cgccgcgcgg
      421 atcgacgtca agtggcgtcg cctccggtga agtcggccgc gcttccgccg cccgcaagtc
      481 cgtggcggcg gcgcgtccac cacacgcgcg cctgctgggc gcgttcgtac gcctgccgcc
      541 cgagcgggtc gaggacgaga tcccacgcgc cgatgtactg gatctcgcga ccgccgaacc
      601 cggtcttgaa gtgggcgatc ccgccggtcg cgaggcccca caggtcatag ctcgtggcgc
      661 ccagctcgcg cgaggtccgg atcgcctccc acttcagcag gtagttggca cgggactcgc
      721 cgccttcggc ggtcatgccg ccgtacggct cgacgacccg ggtcccggcg cgcaccagga
      781 agagagtggc cagcgcctcg ccatccggac ggcgggcgaa caggaggcgg gcgttgccgg
      841 tcggccggta ggcctcccag atgtcgcggt acgcctgctc ggtccggatg aggaagccgg
      901 cccggtcggc ggtctcgcgg tagacccggt agaactcggg cagggcatca ccttccgcat
      961 cggtcacggt gatcccggcg gtccgggcct tgttgacgta ctgccgccat ttcttgcgaa
     1021 gatcgcccca cagggtctcc tcgtcgggcg cgaggtcgat gatccgggtc gcattcggct
     1081 ggatcgggct ggcgggccgc cagccggcgg ctcggaggct gcggcggatg gcgccgtcgg
     1141 agtcggccgg tccgtcgcgc tcgatctccg ggtcgatccg gaggtgcgaa acgcgcccaa
     1201 ccgcggcggt cagggcagcg cggacctcgg cggtgaacgt cccgatcgtg tcgggcgtcc
     1261 aggcggcggt gaccggaccg cgcggggcgt aggcgaatgc ccagggcaac ggccgcgggc
     1321 ggcgtacgag gatctgggcg ccgacgggcg cgccgccgac gggggcggcg ccgacggggg
     1381 cggcgccggc cgcgagcgac tcgcgcgcga ggagtgcccg ggtccagccg ttcaccgcct
     1441 tgacccgcga ccagccggcg agctggaggt acgacccggg attgcctgcg gcgacgaacg
     1501 cgtcccagga ctggtcgttc gcggtgatcg cgatcacggc cgtcgaggcg gcggcgacat
     1561 ccgccggggg tacggtcggc gttgccggct ggtcgtcgtc ggatcgcggc gcggtgccgc
     1621 tcgcggtggc gccggtcacg cccggtgcgg ccggcggatg agctcgagca tgagcccgat
     1681 gatggaggtg agctccgcga tccgcaacac gagggcgagg gccgcggatg tcaggagcca
     1741 gacaagtccg acgaggccca tccggatgac gaggtcgaac cggccggggt tctcgcccat
     1801 cgcaagggtc aggccggcga ggaccccggc cgccgcgagc gacccaccga tcgtgaccgt
     1861 gagggagcgg atcccgagcg acagcagcgg acggaatgcg agctcgggca acgaccgctg
     1921 gagcaggacg agcagcacga ccgtctcgac ccacgcggcg atcgcgatcg cgaggccgag
     1981 ccccggcagc ccgagaggcc cggcgaggac gccggccagc gtcgagttga tgacgaccgc
     2041 gaggatcgcc gcggccaccg gtgtgcgggt gtcctggcgg gcatagaacg cgcgcgccag
     2101 gatcgcgatg agggcgtggg cggtaagccc gagcagaaag gtcaggagcg tcgcagcgat
     2161 caggtcgagc gccgcgcgat cgaagcggct gtagccgaag agcacatcga cgatctcatg
     2221 gcggaggacg atcgcgatcc ctgtcaccgg cagcatcgcg aagatcgtga gccggatcgc
     2281 gcgggagatc agcccggcga atgcgtccag ctccccgacc gcggcgaggc tggacagcga
     2341 cgggagcacg acgattccca gcggcacgcc gatcaggccg atcgggatct ggagcagcgt
     2401 gaacgcgtag ttgaaggccg tgatcgccgc tgttccgagg ccggaggcga tcgaggtcag
     2461 gacgatgaac gtgatctggc cggcgccgag cccgagcgct cgcggggcca tgagcttcag
     2521 cgccttgcgg gcctcggggt cggcgatgtc gatgcggcgc tcgtagcgga agccctgggc
     2581 ccggagcggc gcgagctgga tgagcaggtg gccgagggag cctgcgacga caccgatcgc
     2641 gaggccagtg atcccgaacg acgaggtcag tgcgatcgcc gcgccgatga tcgcgaggtt
     2701 gtagacgatc ggggcgatcg ccgacgcggc gaaccgacct cgggcgttga ggacgctcgt
     2761 cacgagcgac ccgagggcga ggaagatcgg gctgaggatc atgatccgcg tcaggtcgac
     2821 ggtccggctc cactcggcct cggtgaactt cggggtgatg gccgggatga tcgccggagc
     2881 ggcgacgagg aagacgagac cgagcgccag caggccggcg agcatcaggt tggcgatcgt
     2941 cgagacgacc cgccacgccc gcgccgtctc gtcagtggcg agcaggctgg acacgatcgg
     3001 gatgaccgcc gacgacaggg ctccggcggc gacgagctgg aacatcaggt cgggcaggcg
     3061 gaacgcggcg aagaacacgt cgaggtcgga gcccgccggg aagacgtggg cgaagacgac
     3121 gaggcggacc cagccgagga tccgcgagac gaggaaggcg cccgagacga tcaggcccgc
     3181 ccgggccagg gcgcgtgacg agctcacgag cgggcgggtt cggggggctg gcgcgtcgcc
     3241 cgcgggtggg cgccgcgata cgccgagcgg aggcgagccg gcgaaacgtg ggtgtacacc
     3301 tgcgtcgtcg cgaggctctc gtggccgagc agctcctgga cgacccggag gtcggcgcca
     3361 ccctcgagga gatgggtcgc gaaggagtgg cgcaacgtgt gcggcgacac gccggccgga
     3421 aggccggccc gctggcggag gagatccaac cggtagcgga gcccgcggac accgatcgcc
     3481 tcgccgcgat ggttgaggaa gatctcggtc ggtccggcgt cctcgcggcc atggcctcgc
     3541 cggcgcgcca ccaggaccgg tcggccgtcc tcgaggtagc cggccagggc ctggcgggcc
     3601 ggccggccga gcagcccgat ccgctccttg cgccccttgc cgaggacccg gacctcgccc
     3661 cgtcggaggt ccagggcccc gagctcggcc gatgccagct cgctgatccg gagaccggcc
     3721 gcgtacgccg tctcgacgat ggcccggtcg cggatcgcga tcgcgacggt cagctcgctg
     3781 gggctcgccg cgtcgtcctc gagagcgccg tcgaccgcag cgaggagccg ctcgacctcg
     3841 tcgacctcga ggacgcgcgg cagccgtcgg ggcaggcgcg gcgtggcgat cactccccac
     3901 gggtcgccgg gggcgagctc ctcgcggacg gcgaaccggt ggaacgagcg gatcgcggcg
     3961 agccgctggg ccgtcgagga gcgcgcccga cccatcccca ggacggccag atacgtccgc
     4021 aggtccgccc gcgacggcgt ccgccagtcg acgccacgac ccgccagcca gtccaggtat
     4081 acggtcaggg tcgcgccata cgagcgctgg gtgtgggcgg acgtgtcgcg ggccgccagg
     4141 gaccggagga agcgatcgag ggccgactcc gccgtcgcgt cggtcacgcc agggtcacgc
     4201 gtccggggcg gcgccggtcg atcgggtcgg tcacgcgtcg ggcgcggcgc cggctgaacg
     4261 cgtggtccgc cgcgctcctc cccgtgcacc gggccgggcc tcacccgagc gtccgccgcc
     4321 cttggcgccg gaaccgccgg cggagcgccc gcgccgcgcg ccgccgcgag ccggcttggc
     4381 gagagcctcg gggttcggcg gtccgccctc gagcttccgc ccgacgacgt cgacaccctc
     4441 gaccagcggg atggccgcgc cacagcggag gcagatcccc gtgtcgccgt gcttcgcgac
     4501 cgggccgtcg tcggtgtcgt ggagcgcgcc gaccggctcg aagttcgtcg tgtaatcgca
     4561 cttcgggtag ttcgagcagc cgtagaacac gttcccggtc cgacgcgccc ggcgcgggat
     4621 caggtg
//
LOCUS       DATAIW010000060         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_2381, whole genome shotgun sequence.
ACCESSION   DATAIW010000060 DATAIW010000000
VERSION     DATAIW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHM48_02035"
     CDS             complement(<1..908)
                     /locus_tag="VHM48_02035"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2754211.1"
                     /translation="MAVIGIDTHKGTLAACAVDPLGAVVAERTFGCDPEGLAELVRWA
                     RRLAADGRIGVEGSASYGAPIARMLNAAGLEVREVPPHLSRSERGRTRRAGKSDPGDA
                     LAIARVTLREPALPPVRLDDRTEELGLLVDAREELVQSQTRTRNRLHAHLIVLLPGYG
                     RTAAQLVSPRQLAAIEALLAPLGGTRAALARALLEDVRSLGRRAKELQQQIAGLVADH
                     PLLELPGVGVISAAWLISEVGDIDRFGSPAAFAMLAGVAPIPASSGQSQRMRLNRGGN
                     RRLNRALYTIALVQAWHHPPARAYIAR"
     gene            1264..2298
                     /locus_tag="VHM48_02040"
     CDS             1264..2298
                     /locus_tag="VHM48_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX2754212.1"
                     /translation="MRASRLVSLLLLLQSRGGQTAAELARELEVSVRTIHRDVEALSE
                     SGVPIYADRGPHGGIRLVDGYRTRLTGMTADEAEALFLSGLPGPAAELGLGTVVAAAR
                     LKVLASLPVELRARASRLVERFHLDAAGWYHADEPVPLLGPLSEAVWESQRIQVGYDR
                     GDKTVDRILEPLGLVLKAGVWYLIAAADGQPRTYRVSRVARVAALDERFERPAGFDLA
                     AFWAESSSAYERDTPRIEIVLRLAGDRLPRLRGVIGDRPFDTLERLEEPDPDGWLRIR
                     VKLDWPSEVVSQVLAVGPECELLEPIELRERIAAQARRVAAHYEPPPISADEAVRVPE
                     RIAGRMASRP"
     gene            2753..3625
                     /locus_tag="VHM48_02045"
     CDS             2753..3625
                     /locus_tag="VHM48_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX2754213.1"
                     /translation="MTFDPNAQLDPSQVTDVRGSSRLGGRSGGLAVGGGGLGLVIAIV
                     YLLLGGNPGDLLNGGGGQAVDPNTPQSSALASCKTGADANKREDCQIVGYVDSIQAFW
                     ATQVQGYTKAKTVLFSDGVSTGCGDATSSTGPFYCPNDKQVYLDLGFFTELEQRFGAK
                     DAPFARAYVVAHEYGHHVQDLLGDLQAGGNDTGAAGRSVRTELQADCYAGVWAHNAQA
                     TGFIQPLTQAQIAAADAAAKAVGDDHIQQSSGGGVNPDSFTHGTSEQRVRWFTTGYQS
                     GKPGDCDTFNATTL"
BASE COUNT          543 a         1382 c         1329 g          521 t
ORIGIN      
        1 cgcgcgatgt acgcccgagc gggtgggtga tgccaggcct gaaccagagc gatcgtgtac
       61 agcgcccggt tcaggcgccg gttgccgccc cggttgaggc gcatccgctg cgactggccg
      121 gacgaggcgg ggatcggcgc gaccccggcc agcatcgcga acgccgcggg tgagccgaac
      181 cggtcgatgt cgccgacctc cgagatcagc catgccgcgc tgatcacgcc gaccccgggc
      241 agctcgagga gcggatggtc ggcgaccagg ccagcgatct gctgctggag ctccttcgcg
      301 cgacggccga gcgagcggac gtcctcgagc agggcgcggg cgagcgcggc gcgggtgccc
      361 ccgagcgggg cgaggagcgc ctcgatcgcg gcgagctgac gagggctcac gagctgcgcg
      421 gcggtccggc cgtagccggg caggaggacg atcagatggg cgtgcagacg gttgcgcgtg
      481 cgggtctggg actggacgag ctcctcgcgg gcatcgacca gcaggccgag ctcctcggtc
      541 cggtcgtcga ggcggaccgg tggcaaggcg ggttcccgca gggtcacccg ggcgatcgcg
      601 agggcgtcgc ctggatcgct cttgccggcc cggcgggtcc gaccccgctc ggaccggctg
      661 aggtgcggcg gcacctcacg cacctcgagg ccggccgcgt tcagcatccg cgcgatcggg
      721 gcgccgtacg acgccgaccc ctcgaccccg atgcgcccgt cggccgccag ccggcgcgcc
      781 cagcggacga gctcggcgag gccctccgga tcgcacccaa acgtgcgctc ggccacgacc
      841 gcgcccagcg gatccacggc gcacgccgcg agcgtgccct tgtgggtgtc gatcccgatg
      901 accgccatga tcgcctcctc gacgtccccc gttacgatgg gcgcgggtcg gccgggcagc
      961 agtgcgttga ggtcaaagcc acgctcctat gaggccacgg ccggcccgct tgctccggga
     1021 acgagggcac ttgcgaggca ggccaccacg cggggcactc acaggctggg ccaacccgct
     1081 cccatcgcca gcaccttccc acgtcagcga gaagaccgga tcagttacgc actcacaggc
     1141 atccgagcct gctcgctgcg gtcctcgggc gcgccttgcc accgccgccc cttcgcgacg
     1201 aacgcagtct tggcggttcg gtgtagctga caattcgtgt cacgatttgg tatcgtgccc
     1261 gcgatgcgtg cgtcccgcct cgtctcgctc ctgctcctcc tccaatcccg cggcggccag
     1321 accgcggccg agctcgcccg tgagctcgag gtgtccgtcc ggacgatcca tcgcgacgtc
     1381 gaagccctga gcgaatccgg cgtaccgatc tacgccgacc gcggaccgca cggcggaatc
     1441 cggctcgtcg acggctaccg gacccgcctc accgggatga ccgcggacga ggccgaggcg
     1501 ctcttcctct cgggcctgcc ggggccggcg gccgagctcg gtcttggtac ggtcgtggcc
     1561 gcggcccggt tgaaggtcct cgcctcgctg ccggtcgagc tgcgggcgcg cgcctcgcgg
     1621 ctcgtggagc gattccacct cgacgcggcc ggctggtacc acgcggacga accggtaccc
     1681 ctcctcggac ccctctcgga ggctgtctgg gagagtcagc gcatccaggt cggctatgac
     1741 cgcggcgaca agacggtcga tcggatcctc gagccgctcg ggctcgttct caaggccggt
     1801 gtctggtacc tcatcgcagc cgccgacggc cagccgcgca cgtaccgcgt ctcgcgggtc
     1861 gcccgggtgg ccgcgctcga cgagcggttc gagcggccgg ccggcttcga cctcgccgcg
     1921 ttctgggccg agtcgagctc ggcctacgaa cgcgacacgc cgcggatcga gatcgtcctg
     1981 cgcctcgccg gcgatcgatt gccccgtctt cgcggggtga tcggcgaccg cccgttcgac
     2041 acgctggagc ggctcgagga gccggacccg gacggctggc tgcggatccg ggtcaagttg
     2101 gattggccga gcgaggtggt ctcgcaggtt ctggccgtcg gtcccgagtg cgagctgctc
     2161 gaaccgatcg agctccgcga gcggatcgcg gcacaggctc ggcgcgtcgc ggctcactac
     2221 gagcctcctc ccatatcggc cgacgaagcc gtccgggtcc ccgaacggat cgccggcagg
     2281 atggccagcc gtccctagtg acgacaccta ggccgggacg ttggtcgagt ctccgcaggc
     2341 cgttcgtcgg acgaccgtcg ccggccgggc gagccttgtc ggcgtggtcg acggtccttc
     2401 gtcggtggag cccgccagca gccgacgatc ccgcgcggcg cgctcgagat cgcgaacgcg
     2461 atcggcgtgg atggcccacg cgacggcctc tgaaagaaac acttcgcacc tcgtatgggg
     2521 cgatcgggat gaccgcttca ctcactcccg gagcatgccg gacatacctg tcagaacgtg
     2581 tcatgagtca tctggagccg gcgatttcga gcgagcccgc gaccgggcgc ggaccgtcgc
     2641 tcggcacagt cgcaatgcga ttgcgacgat ggacgcgccg gccagtgggc agcggatggc
     2701 cacaatcggc tacaactcca gcggttcccc agtgcccgga ggtcagccca ggatgacatt
     2761 cgatccaaac gcccagctcg acccatcgca ggtcaccgac gtccgcggct cgagccgcct
     2821 cggcgggcgg agcggcgggc ttgccgtcgg cggcggcggc ctcggcctcg tcatcgcgat
     2881 cgtctatctc ctgctcggcg gcaacccggg cgacctgctc aacggcgggg gcggccaggc
     2941 cgtcgacccg aacacgcccc agtcgtcggc cctcgccagc tgcaagaccg gcgccgacgc
     3001 gaacaagcgc gaggactgcc agatcgtcgg ctacgtcgac agcatccagg cgttctgggc
     3061 cacccaggtc cagggctaca ccaaggcgaa gacggtgctc ttcagcgacg gcgtgtcgac
     3121 cggctgcggc gacgcgacgt cgtcgaccgg cccgttctac tgcccgaacg acaagcaggt
     3181 ctacctcgac ctgggcttct tcaccgagct cgagcagcgc ttcggggcca aggacgcccc
     3241 gttcgcccgg gcatacgtcg tcgcccacga gtacggccac cacgtccagg acctgctcgg
     3301 cgacctgcag gccggcggca acgacacggg cgccgcgggt cgatcggtcc ggaccgagct
     3361 ccaggccgac tgctatgccg gcgtctgggc ccacaacgcc caggcgaccg ggttcatcca
     3421 gccgctgacc caggcgcaga tcgcggcggc cgatgctgcc gcgaaggccg tcggcgacga
     3481 ccacatccag cagtcgagcg gcggcggggt caatcccgac tcgttcaccc acggcacgtc
     3541 ggagcagcgc gtccgctggt tcacgacggg ctaccagtcg ggcaagcccg gcgactgcga
     3601 cacgttcaac gcgacgaccc tctgaggtca tcgcccgggc gtcaggcggg ggccagggcc
     3661 ccggctccga ccggcatcgc gacgcggccc tcgaccgccc cggacgcggc ggtcgaccgg
     3721 tcgatcgcgg gcgtctcttg cgacgtcgga gcgtccgggc ggcgcgcctc gccga
//
LOCUS       DATAIW010000061         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_179964, whole genome shotgun sequence.
ACCESSION   DATAIW010000061 DATAIW010000000
VERSION     DATAIW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179964"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VHM48_02050"
     CDS             <1..1343
                     /locus_tag="VHM48_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2754214.1"
                     /translation="ANVTDDPARGPETSRDDRAADDRRRATRRGSDADAEPATSNPRE
                     PLPQVDPPAWSWGDRRGGPGPGRAYGRGPWRGRQNAGPRPAWLPEDEPWPPPRRRPRR
                     GFGCLFGFLFLAGVLGLLSFGTSLAGRVLDAPGPVGLVVRFASFVVVVAVVVGLARAA
                     HAIRGSGTVLDALVDQAARVEAGDYGARVETIGPVPAPVRGLARGFNTMAARLEADEA
                     QRRTLLADVTHELRTPLAVIQGSIEAILDGVHPADEAHLSAILDETRTLDRLIEDLRT
                     LALSESGALSLHREPTDLSVLATDVATSFAATAAAAGVELATSLDDDLPLLDVDPVRI
                     REVIGNLVANALRHTPAGGRVAIDAHRSPAIGGDGGEAIEIVVLDTGSGIDAELLPHV
                     FDRFARGAGSTGSGLGLSIARGLVELHGGTIAASSPAGGGAAIRIRLPVAPPQR"
     gene            1488..3128
                     /locus_tag="VHM48_02055"
     CDS             1488..3128
                     /locus_tag="VHM48_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX2754215.1"
                     /translation="MFRRLRAPRRSMFLGAIVAVAFIAISAVPVAAADFPPKDSRYHN
                     YAEMVADIHATETAHPDIVHVFSIGKSYQGRAIWAAKISDNVATDENEPEILFDALHH
                     AREHMTVEQALYLLHLLANHYGTDSQITNLVDSREIWIIFAVNPDGFVYDLTCTSGSH
                     APYCAWRKNRQPNAGSTAVGTDLNRNYGYAWGCCGGSSSNPASIIYRGRSRWSAPETR
                     VVRDFVLSRIVGGIQQIKAHITFHTNGELILWPYGYTKTDVPIDMTSVDHHAFYRMGK
                     AMAARNGYTAKQSSDLYITDGDQIDWLYGSQRIFSFTWELFPTETPTVWGDHYPADEN
                     IATQTARNRSALLYFLTVGGCPHGVLGLAKTDCGPFFDDAEISRGWVVDPDGTDTAPA
                     SARFSRANPELTVYAGVHIQPTWVTSGRDAFVTGAAAGSTPNANDLDGQTTIRSVPIA
                     LPEAPGNLSFRYTFGHGPGSATDTLQVFIEDETATRTLVWSVAGSATTVAASWKTASI
                     PLTTWAGSPIRIVIQATDRDANSLVEALVDDIRVQRPN"
     gene            3291..4355
                     /locus_tag="VHM48_02060"
     CDS             3291..4355
                     /locus_tag="VHM48_02060"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX2754216.1"
                     /translation="MRRRRFGRFGLGLSTAVLGLFLAITPTLACGGLIGPNGAVNLLR
                     TTTLAGYHDGIEHYVTAFQFAGGGGAFGSITPLPGIPTSVVKGGDWTLQRLIRETNPP
                     VPEAFDAARAASSAGAVELMTVKIDALDLTVLKGGAADIGVWANDHGFRLPPDAPEVL
                     DFYAARSQIFLAAAFDADAAQNRGQQLGDGTPVHITIPTANPWVPLRILALGKAGTEP
                     VQADVYLLTDRAPALLPAPSGSNGIRLDHSAAASASLLADLQSDRGMDWVPSSGWLTK
                     VAVDGTAAQLGFDLAIDASGAGRPSAIDAGFTMPGAVIPPASFDLSRLLLALAFVAIG
                     LVGVALIGRARPSAGRPIGW"
     gene            4363..>4766
                     /locus_tag="VHM48_02065"
     CDS             4363..>4766
                     /locus_tag="VHM48_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2754217.1"
                     /translation="MRGGRSTVGLALGVGLAIAVLTGIAANAVARSHDPIEIPITIHY
                     SHYEPATMTVPAGRPVTFIITNTDPIDHEWIVGDAATHERHRTGTEPVHDARPTEVSI
                     PAGTVKRTTVTFAANGSELFICHLPGHEAYGMV"
BASE COUNT          684 a         1809 c         1585 g          688 t
ORIGIN      
        1 gagcgaacgt gaccgacgac cccgctcgcg gacccgaaac gagtcgcgac gatcgggcgg
       61 cggatgatcg tcggcgggca accaggcgcg gctccgacgc cgacgccgag cccgccacct
      121 cgaacccccg cgagccgctg ccgcaggtcg atccgccggc ctggtcctgg ggcgaccgcc
      181 gtggcggccc gggcccgggc cgtgcctacg ggagaggccc gtggcgcggt cggcagaacg
      241 ccggcccgcg accagcgtgg ctgcccgagg acgagccgtg gcccccgccc cgcaggcggc
      301 cgcggcgcgg cttcggatgc ctgttcgggt tcctcttcct cgccggcgtc ctcggactcc
      361 tgtcgttcgg gacgagcctg gccggccgcg tccttgatgc tccgggtccg gtcgggctcg
      421 tcgtccggtt cgcgtcgttc gtcgtcgtcg tcgcggtcgt cgtcgggctt gcccgggccg
      481 cccacgcgat ccgcggctcg gggacggtcc tcgacgcgct cgtcgaccag gccgctcgcg
      541 tcgaggcggg cgactacggc gcccgcgtcg agaccatcgg accggtcccc gctccggtcc
      601 gcgggctcgc ccgggggttc aacacgatgg ccgcccgtct cgaggcggac gaggcccagc
      661 gccggacgct cctcgccgac gtgacccacg agctccggac gccgctggcc gtcatccagg
      721 gcagcatcga ggccatcctc gacggcgtcc acccggccga tgaggcccac ctgtccgcca
      781 ttctcgacga gacccgcacc ctcgaccgcc tgatcgagga tctccggacc ctcgccctgt
      841 cggagtccgg cgcgctctcg ctccatcgcg agccgaccga cctgagcgtc ctcgcgacgg
      901 acgtcgcgac ctcgttcgca gcaaccgccg ccgccgcggg cgtggagctc gcgacgagcc
      961 tcgacgacga tcttcccctg ctcgacgtcg atcccgtccg gatccgcgag gtcatcggga
     1021 accttgtggc caacgccctc cgccacacgc cggccggcgg ccgggtggcg atcgatgctc
     1081 accggtcgcc cgcgatcggt ggtgatggcg gcgaggcgat cgagatcgtc gtcctcgaca
     1141 cgggatcggg catcgacgcg gagctcctgc cccacgtctt cgatcgcttc gcccgcggtg
     1201 ccggctcgac cggcagcggg ctcgggctgt cgatcgccag gggcctcgtc gagctccacg
     1261 gcgggacgat cgcggcgtcg tcgccggccg gcggcggagc cgcgatccgg atccggttgc
     1321 cggtcgcacc gccgcagcgc tgatcgcacc gcgctcgcgg accgggacgc cgggcgccgg
     1381 gacaccgccg gcactccggc agccgcccga ctgggtcatt ggtcctgtcc cgacccgtgt
     1441 ctgaggtcgg gttgcgtccg ggcaccaccc tccggcaccc tagtcacatg ttccgacgtc
     1501 tgcgcgcccc tcgtcgatcg atgttcctcg gcgccatcgt ggccgtcgcg ttcatcgcca
     1561 tctcggccgt ccccgtggcc gctgccgact tcccacccaa ggactcgcgc tatcacaact
     1621 atgccgagat ggtcgccgac atccatgcca cggagacggc gcatcccgac atcgtccacg
     1681 tgttcagcat cggcaagagc taccagggcc gcgccatctg ggcagccaag atcagcgaca
     1741 acgtcgcgac cgacgaaaac gagccggaga tcctgttcga tgccctgcac catgcgcgcg
     1801 agcacatgac ggtcgagcag gccctgtacc tgctccatct cctcgccaac cactacggca
     1861 ccgacagcca gatcacgaac ctcgtcgaca gccgcgagat ctggatcatc ttcgccgtca
     1921 accccgacgg cttcgtctac gacctgacct gcacgagcgg cagccatgcg ccgtactgcg
     1981 cctggcgcaa gaaccgccag ccgaacgccg gcagcacggc cgtcgggacg gacctgaacc
     2041 ggaactacgg ctatgcgtgg ggctgctgcg gcggttcatc gagcaacccg gcctcgatca
     2101 tctaccgcgg tcggtcgcgc tggtccgccc cggagactcg cgtcgtgcgc gacttcgtcc
     2161 tgagccggat tgtcggcggc atccagcaga tcaaggcgca catcacgttc cacaccaacg
     2221 gcgagctgat cctgtggccc tacggctaca cgaagaccga cgtcccgatc gacatgacca
     2281 gcgtcgacca ccacgcgttc tatcgaatgg gcaaggcgat ggccgcccgg aacggctaca
     2341 cggccaagca gtcgagcgac ctgtacatta ccgacggaga ccagatcgac tggctctatg
     2401 gcagtcagcg gatcttctcg ttcacctggg agctcttccc gaccgagacc ccgacggtct
     2461 ggggcgacca ctacccggcc gacgagaaca tcgcgaccca gacggcccgc aaccggagcg
     2521 cgctgctgta cttcctgacg gtcggcgggt gtccgcacgg ggtcctgggc ctggcgaaga
     2581 cggactgcgg cccgttcttc gatgacgccg agatctcccg tggctgggtc gttgacccgg
     2641 acgggacgga cacggccccc gcttcggcac ggttcagccg ggccaacccc gagctgacgg
     2701 tctacgccgg cgtccacatc cagccgacct gggtgacctc gggccgggat gcgttcgtca
     2761 cgggcgcggc ggccggatcg accccgaacg caaacgacct cgacggccag acgacgatcc
     2821 ggagcgtccc gatcgccctg ccggaggcgc ccggcaacct gtccttccgc tacaccttcg
     2881 ggcacggccc cggcagcgca accgacacgc tccaggtctt catcgaggac gaaaccgcga
     2941 cccgaacgct cgtctggtcc gtggccgggt cggcgaccac cgtcgcggcc tcctggaaga
     3001 ccgccagcat cccgttgacg acctgggccg ggtcgccgat ccggatcgtc atccaggcga
     3061 ccgaccgaga cgcgaacagc ctggtcgagg cgctcgtcga cgacatccgg gtccagcgcc
     3121 cgaactgaat cctcgggctg atggcctcgc ggagctcggc ctcaggcctc ggagggccgg
     3181 gtcgcaattg agctctcgct gacgcgcggc ggaacccgct ccgccggtcg ggcgtcaggg
     3241 gccgcaaccg ccggcccgcc cgtggccggc cagcccagga gggcgacgcg atgcggcgac
     3301 gacggttcgg tcggttcgga ctcggcctct ccacggcggt cctcggcctc ttcctggcga
     3361 tcaccccgac cctggcctgc ggcgggttga tcggcccgaa cggggccgtg aacctgctcc
     3421 ggacgacgac cctcgcgggc taccacgacg ggatcgagca ttacgtcacg gccttccagt
     3481 tcgccggtgg tggcggcgcc ttcggctcga tcactcccct ccccggcatc ccgacgagcg
     3541 tcgtcaaggg tggcgactgg acgctccagc ggttgatccg tgagacgaac ccgccggtgc
     3601 ccgaggcgtt cgatgcggcg cgagcggcca gctcggctgg agcggtcgag ctcatgaccg
     3661 tcaagatcga tgccctcgac ctgaccgtcc tcaagggcgg cgccgcggac atcggcgtct
     3721 gggcgaacga tcacggcttc cgccttccgc ccgacgcgcc ggaggtcctc gacttctacg
     3781 ccgcgcgcag ccagatcttc cttgccgccg cgttcgacgc cgatgccgcg cagaaccgcg
     3841 gccagcagct cggcgacggg acgccggtcc acatcacgat cccgaccgcg aacccgtggg
     3901 tcccgctccg gatcctcgcc ctgggcaagg cgggcacgga gcccgtccag gcggatgtct
     3961 acctcctgac cgaccgggct ccggcgctcc tgccggcgcc cagcggatcc aacgggatcc
     4021 gtctcgacca cagcgcagca gcgtccgcaa gcctcctcgc cgacctccag tccgaccgtg
     4081 gcatggactg ggtgccgtcg tcgggctggc tgaccaaggt cgcggtcgac gggaccgctg
     4141 cccagctcgg cttcgacctc gcgatcgatg cgtcgggcgc cggtcggccg tcggcgatcg
     4201 atgccgggtt cacgatgccg ggcgcggtca tcccgccggc gtcattcgac ctgagccggc
     4261 tcctgctggc cctcgccttc gtcgcgatcg gcctcgtcgg cgttgcattg atcggccggg
     4321 cgcggccgtc ggccggcagg ccgatcggct ggtaggacgg cgatgcgcgg cgggcggtcg
     4381 acggtcgggt tggcgcttgg agtcgggctg gcgatcgcgg tcctgaccgg gatcgcggcc
     4441 aacgccgtgg cccgctccca cgacccgatc gagatcccga tcacgatcca ttactcgcac
     4501 tacgagccgg cgacgatgac cgtgccggcc ggccggcccg tgacgttcat catcaccaac
     4561 accgacccga tcgaccacga gtggatcgtc ggcgacgccg ccacccacga gcgtcatcgg
     4621 accggcaccg agccggtcca tgacgcccga ccgaccgagg tctcgatccc ggctgggacc
     4681 gtgaagcgga cgaccgtgac gttcgccgcc aacggctccg agctcttcat ctgccacctg
     4741 ccggggcacg aggcgtacgg gatggt
//
LOCUS       DATAIW010000062         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_151725, whole genome shotgun sequence.
ACCESSION   DATAIW010000062 DATAIW010000000
VERSION     DATAIW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151725"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1207)
                     /locus_tag="VHM48_02070"
     CDS             complement(182..1207)
                     /locus_tag="VHM48_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX2754218.1"
                     /translation="MTGPALEVLDGGLLTTVQDAGRPDWTHLGVPASGAADPWSLAVA
                     NLLVGNDPGRAALEMTLVGPTLVVRGAITVGLAGADLGARIRDGRRLAPGRSHRLGAG
                     DFVEIPGDSRSLPSTGARAYLAVPGGVDVPLVLGSRSTCLAGGFGGLDGRALRLGDGI
                     WPALAAAAGSAPVGAVRPELVWPEADGADRFDAVVTPATLRVVVGPAPGLDALGTIDW
                     RVGTAANRVGIRLDGEPLPDGIGGETTTHGVPWGAIQVPPDGRPIILGADHQTTGGYR
                     VVAVVISADLAVLGQLRPGASLRLVAVDRPAALRALRARRDALSAGAAALRDAAGWDA
                     LIDSAGG"
     gene            complement(1204..1926)
                     /gene="pxpB"
                     /locus_tag="VHM48_02075"
     CDS             complement(1204..1926)
                     /gene="pxpB"
                     /locus_tag="VHM48_02075"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX2754219.1"
                     /translation="MPDRGPSGSPPSGSPSIEPVGDAGLLISLGSTTDPDVAAWAQAI
                     AAAIDTARAATPAIGRPVPAHASVLVPFDPLAIELEDAIDFARQAVVAAEAGSSVAGA
                     GAGADDGPVIEIPVRYGGPDGPDLDAVAGLHGLRPDEVVDLHASARYHVLFLGFGPGF
                     GYLGGLPAALVTPRRASPRERVPAGSVGIAGEHTAVYPLAMPGGWQLIGRTDRVLFDP
                     DRPDPALLRPGATVRFVPLRDE"
     gene            complement(1919..2704)
                     /locus_tag="VHM48_02080"
     CDS             complement(1919..2704)
                     /locus_tag="VHM48_02080"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX2754220.1"
                     /translation="METPAGNSRSIDLNADVGESFGPWPMGADDALVPLVSSVNIACG
                     AHAGDPATILRTAALAARLGVAIGAHPGYPDLAGFGRRDLDMTAADLRASLVVQIGAV
                     QAAARLAGTAVRHVKPHGALYNRTARDPGIATTIAEAIRDLDPALVLVGLAGSASIAA
                     GRTAGLAVSEEAFADRRYEADGSLRSRLRPGALLDPADAAAQALGIVRDGSVVAGDGS
                     RLTVRADTICIHGDSPDAVAIARAVVAALGDAGFRIAARSLDA"
BASE COUNT          393 a         1051 c         1105 g          319 t
ORIGIN      
        1 cgatcgatcc gacgccgtcg tcacgcccgc caccttgcgc gtgatcgtcg gcccggcccc
       61 cggcctcgac gccctcggga cgatcgactg gcgggtcagg gtcgtatcgc tcatgtcagt
      121 gattgttcgc cggaacgatg acgttcggat gtcgtggtcg gtggatcatc gcagacgcgc
      181 gtcagccgcc ggctgaatcg atcagggcgt cccaaccggc cgcgtcacgg agggccgcgg
      241 caccggcgct gagcgcgtca cggcgggcgc gcagggcgcg gagcgctgcc gggcgatcga
      301 ccgcgaccag ccggagagac gcgccgggac ggagctggcc gagcaccgcc aggtcggccg
      361 agatcacgac cgcgacgacg cgatacccgc cggtcgtctg gtggtcggcg ccgaggatga
      421 tcgggcggcc gtctggcggg acctggatcg ccccccaggg aacgccgtgg gtcgtcgtct
      481 cgccaccgat gccgtcgggc aggggctcac cgtcgaggcg gatgccgacc cggttcgcgg
      541 cggttccgac ccgccagtcg atcgtcccga gggcgtcgag gccgggggcc gggccaacga
      601 ccacgcgcaa ggtggccggc gtgacgacgg cgtcgaatcg atcggcaccg tcggcttccg
      661 gccagaccag ctccggccgg actgcgccga cgggcgcgga ccccgcggcc gcagcgaggg
      721 ccggccagat cccgtcgccg agccggagcg cgcggccgtc gagcccgccg aacccgccgg
      781 cgagacacgt cgagcgtgag ccgagcacga gcggaacgtc gacgccgccg gggaccgcga
      841 gatacgccct ggctccggtc gagggaaggg atcggctgtc gcccgggatc tcgacgaaat
      901 cgcctgcgcc gagccgatga ctccgtcccg gcgcgagacg ccgtccgtcc cggatccggg
      961 cgccgaggtc cgcgccggcc agcccgaccg tgatcgcccc gcggaccaca agggtcggcc
     1021 cgacgagcgt catctcgagg gcggcccggc cgggatcgtt gccgacgagc aggttcgcga
     1081 ccgcgaggct ccacggatct gccgcgccgg atgccgggac gccgaggtgg gtccaatcgg
     1141 ggcggccggc gtcctggacc gtggtcagca gtccgccgtc caggacctcg agcgccggcc
     1201 cggtcactcg tccctcagcg ggacgaaccg gaccgtcgcc ccgggtcgca gcagcgccgg
     1261 atccgggcga tccgggtcga agagcacgcg gtccgtccgg ccgatcagct gccagccgcc
     1321 cggcatcgcg agcgggtaca cggcggtgtg ctctccggcg atcccgacgc tccccgccgg
     1381 gacgcgctcg cgcgggctgg ctcgacgagg cgtgacgagg gccgcgggaa gcccgccgag
     1441 atagccgaac cctggcccga acccgaggaa caggacgtgg tagcgggccg aggcgtggag
     1501 gtcgacgacc tcgtcgggtc gcaggccgtg cagcccggcg acggcgtcga ggtcggggcc
     1561 gtccgggccg ccataccgaa ccgggatctc gatcaccggc ccgtcgtccg ccccggcgcc
     1621 ggcgccggcg accgaagacc cggcctccgc ggcgacgacg gcctgtcggg cgaagtcgat
     1681 cgcgtcctcg agctcgatcg cgagcgggtc gaacgggacg aggaccgagg cgtgagccgg
     1741 aaccggtcgt ccgatggccg gcgtggccgc gcgggccgta tcgatggccg ccgcgattgc
     1801 ctgggcccat gccgcgacgt cggggtccgt ggtcgatccg agcgagatca ggagcccggc
     1861 gtcgccgacc ggctcgatcg acggcgaacc ggatggcggg gagccggacg ggccccggtc
     1921 aggcatcgag ggaacgggcc gcgatccgga acccggcatc gcccagggcc gccacgaccg
     1981 cccgagcgat cgcgaccgcg tcggggctgt cgccgtggat gcagatcgtg tccgcgcgaa
     2041 cggtcaggcg cgacccgtcg ccggcgacga ccgatccatc ccggacgatc ccgagggcct
     2101 gggccgccgc gtcggccggg tcgagcagcg ccccgggacg cagccgcgag cggagcgagc
     2161 catccgcctc gtagcggcga tcggcaaagg cctcctcgct caccgccagc ccggccgtcc
     2221 gcccggccgc gatcgacgcc gagccggcca gcccgacgag aacgagcgcc gggtcgaggt
     2281 cgcggatcgc ctcggcgatc gtcgtcgcga tcccgggatc acgcgcggtc cggttgtaga
     2341 gcgcgccgtg cggcttgacg tggcggaccg ccgtcccggc aagacgcgcc gccgcctgga
     2401 ccgcgccgat ctggacgacc agcgaggccc ggagatcggc cgccgtcatg tcgaggtcgc
     2461 gccgcccaaa cccggcgagg tcgggatagc ccgggtgggc tccgatcgcg accccaagcc
     2521 gcgccgccag ggcggcggtc cgcaggatcg tcgccggatc gccagcgtgc gctccgcagg
     2581 cgatgttgac gctgctgacg agcgggacga gcgcatcgtc ggcacccatc ggccacggcc
     2641 cgaacgactc gccgacatcg gcgttcaggt cgatcgatcg actgttcccg gccggcgttt
     2701 ccatggcgac gatgctaccg ccgcggacgg cggtcggaca ggaacgacgg agcgtgatcc
     2761 gcgcccgcta cggcgcgccg ctgggcagct ccgcggccgc cgatgcggcg ggtgtcggag
     2821 tgggcgccgc ggtcggcgtc ggagtgggcg ccgcggtcgg cgtcgggg
//
LOCUS       DATAIW010000063        12056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_151764, whole genome shotgun sequence.
ACCESSION   DATAIW010000063 DATAIW010000000
VERSION     DATAIW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151764"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VHM48_02085"
     CDS             complement(<1..1058)
                     /locus_tag="VHM48_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2754221.1"
                     /translation="MTKGRNRAGGRIVVDGRPIEFAEGDSVAIAIIRAGEVPGRGGAI
                     CLAGDCGNCLAAVDGVAYVRTCQVPPQPGMVVERQLGSGKPPLPSVELGDLTRPPVGE
                     RIPVRRADVDLVIAGQAEAGNAAGEAARSTGRAVVFLDAQAGQEVVAIYPGPSVVVRE
                     PGRMTHLRADEVVVATGAAELQPVCEGSDLAGILTPKAAERLGAAGVDLGRVERVGRE
                     LIRFEGDDRGRVSAVVTRAPDGSEMTTECDTVVVDLGRAPRDVLARMSRDDAVRVVGS
                     AADDHPLPPAPTAGVVCPCMGTTVDDLETAFAKGYSNLELLKRASWAGLGPCQGGACL
                     PHVRAFIAARTGSVPDPFT"
     gene            complement(1055..1471)
                     /locus_tag="VHM48_02090"
     CDS             complement(1055..1471)
                     /locus_tag="VHM48_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2754222.1"
                     /translation="MSVFVDTSMFYAAIDAGDIDHERAKRTLSGESGDLLTTDHVLFE
                     TWRLLRKRLGRPVAERFFETIRAGLAEFEYIEPRDVEAAWAIGLGYPDQDFSIVDRTS
                     FAVMERLGVRRVATFDSDFAIYRFGPRRDRAFEIVA"
     gene            complement(1475..1741)
                     /locus_tag="VHM48_02095"
     CDS             complement(1475..1741)
                     /locus_tag="VHM48_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754223.1"
                     /translation="MMMTRLQVTMETGMRRRAKRRADELGISLAEFVRRSVDQALDQP
                     DRPKPDISAIFGLGNSGGSNVAKHKDEYLDEAVEADYLKTKRER"
     gene            complement(1769..4237)
                     /locus_tag="VHM48_02100"
     CDS             complement(1769..4237)
                     /locus_tag="VHM48_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754224.1"
                     /translation="MKSSAQVVVIGGGVVGASVLYHLTKAGWTDAILLERRELTAGST
                     WHAAGGMHTLNGDPNVSKLQQYTIQLYEEIERVSGQNCSIHLPGGLMLADTEVRMDFL
                     RMAQARGRYLGMDLELITPKEAQVVFPLLDPQYFVGALYDPVEGHVDPSGVTRAYVKC
                     AQLLGAEVRQHTMVTGLSQRPDSTWDVHIENGDPIHAEHLVNAGGLWAREVGRLVGIE
                     LPVLAMEHMYLLTVDMPEVAANVAKTGKEMPMALDFAGEIYIRQEGGGMLLGTYEQAA
                     VPWSPKETPWDFGPQLLKPDLDRITPELQVAFKHYPAMGTTGIRKVVNGPFTFSPDGN
                     PLVGPVRGLRGYWVACGVMAGLSQGGGVGLALSAWMTAGDAGDSGMDIWAMDVARYGD
                     YATLAYTNAKVQENYRRRFRITFPNEELPAARPLLTTPIHDRLTAANAVWGATAGLEH
                     ALWFQKAGLEPKEDVTFRRSNAWDCVAEEVAAVRERVGMTEISNYAKYRVTGPGAEAW
                     LSSLLTARMPAPGRITLTAMLNDAGRIVGEFTVARASEADEFFLFGSLPAETHHARWF
                     IHHLPADGSVRFEVLGLGLVGLSLTGPKSRDVLAAVAHDLDLSTAAFPFMTFRRVDLG
                     MQPALVARINYSGDLGYELWVAPEHQRALFDRIVEAGAPHDLRLFGMRALMSLRLEKS
                     YGTWFREYRPIYTPAEGGITRYLRLDHDFVGRAAHEAEMAAGGPKRRLVTFVVDPQPD
                     DPADVIGDEPVWHDGTVVGWITSGGYGHYVKQSIALGYVPTDLATPDGPGGQGYEIEI
                     IGRRRPARIQPEPLFDPQGLRMRQ"
     gene            complement(4297..4704)
                     /locus_tag="VHM48_02105"
     CDS             complement(4297..4704)
                     /locus_tag="VHM48_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2754225.1"
                     /translation="MFVVDASIALAWCFADESSDATDGVLRQLLTEGGLVPAHWPMEV
                     ANALRFGERRGRIDEAKLSVARSLLTDLPVDVVPIDLSAALDVVDLARRHDLTVYDAV
                     YLDLAATRGLALATVDRRLAAACREASVALVAA"
     gene            complement(4704..4943)
                     /locus_tag="VHM48_02110"
     CDS             complement(4704..4943)
                     /locus_tag="VHM48_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2754226.1"
                     /translation="MRSIGAYEAKTHLPRLLDEVARGERITITKHGRPVAVLVPPGVV
                     RDIDVDAFMARMREFRKGNVLGDVTIRELIDEGRR"
     gene            complement(4999..6777)
                     /locus_tag="VHM48_02115"
     CDS             complement(4999..6777)
                     /locus_tag="VHM48_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2754227.1"
                     /translation="MIDHRHAIELAATAIDFVLDDRDRGQLEEHLHSCESCRFESSAM
                     RRDASSLASLPDLEPPTWVRRAIGRPHGPRRAVLLIAAALVLAATVGVAFAVGATLRD
                     HSELNPQPSSVPRATVTSGTPRPSVETSPSASPSIGPPPVGKPSSFPAGAGSARFAAG
                     PDGGVWVLADHPGDLGPDAASTSVLGLLDRDGRPRPGWPIAFDGWSCDADAPPHGLPV
                     AADGSIRLVCASDTTSEGPQRHVALAFGADGQALPGWPVELPLADLNLPPVVVGNELR
                     VLAREVASTDGTGSAQPAAWWLIGVSANGDVRVGQRFEVADAAGNFDVRLAADGVAYR
                     VAIVDQPTSDVPVVSVIDAIDLDGARPGWPVTVEGSASHPVVGPNGRLVITRRIGTGD
                     GARAQTMTIDPGGGDATATSDGLPIDPLDDRTGAGARVIPPVVASDGTVFVIGSDKTG
                     QRAFVVDPPLAGRGVPPARLKMPLQPMGSCNGVDIGCGVWRTLPVVGPDGTLYVPESA
                     VGEGGGLSASSGGSLVATAPDGRVRDGWPVFLPDPMAGYWSVIVRSDGTVDALAVYPT
                     DGGAEWTLLILGPDGTTRASTPIIRP"
     gene            complement(6774..7340)
                     /locus_tag="VHM48_02120"
     CDS             complement(6774..7340)
                     /locus_tag="VHM48_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754228.1"
                     /translation="MEPGADPDLAVATMLVSDVPNALGAQTGDAYERLSAQVYGFLVR
                     TVRDPDAAADLLADVFTRLLVEERRGRWPDQPRAWLYRAATNLAMSRGRHLQVVARVD
                     RVLRTRYMEPRDASPDVEVLRRERRGDLDRALDQLTTDARVALLLAAQGVDGAAISQA
                     IDRTESATRTLLSRSRVRLRELLREVER"
     gene            complement(7413..9014)
                     /locus_tag="VHM48_02125"
     CDS             complement(7413..9014)
                     /locus_tag="VHM48_02125"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2754229.1"
                     /translation="MTDEELMAAALGGAAPAEAPTGEVAPRRRGGRATGERSRLPQQR
                     PWAQPRIRYAPTEIVSADELESIHDASLRVLEEIGMDFLDDGARDVLRAAGARVEAGT
                     QRVRFDRDLVLERIKTAPSEFTLHATNPDHNLQIGGNWTAFGSVASAPNVADLDRGRR
                     IGNRADYQNLIRLCQMLNPVHFFAGYPVEPIDIHASIRHLDALWDLLTLADKPIHAYS
                     LGRQRITDAIEMARIARDIDDATLDREPSLFTVINSSSPLRLDTPMLHGILEMSARNQ
                     VIVMTPFTLAGAMAPVTLAGALAEQNAEALAGMVLTQVVRPGAPVVYGGFTSNVDMQS
                     GAPAFGTPEYMKTAMIGGQLARRYNVPYRSSNVSAANAVDAQAAYESVFSLWGAISGG
                     VNLLMHGAGWMEGGLHAGYEKVILDAELLGMVEAFLDPVVVDEDTLAFSAMEEVGPGG
                     HFFGAAHTQSRFRTAFHKPMLSDWRNYETWEEAGSPQAPAKANAIWKELLNAYEPPAM
                     DEAIKEALAAFVDRRKSEGGVPTDF"
     gene            complement(9342..10868)
                     /locus_tag="VHM48_02130"
     CDS             complement(9342..10868)
                     /locus_tag="VHM48_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX2754230.1"
                     /translation="MTGTAERERHRTTTAEWIERAAAITPRTDAFIDGRFVPAASGRT
                     FEDIAGRDGSTIAQVAEGGAEDVDRAVAAARHSFDDRRWADRKPLERKKVLLRFADTI
                     RDHLDELALLESLDVGKPIRDTLAVDVPSAAATIQWYAETIDKVYGEVGPTGPDALSL
                     VTREPIGVVAAIVPWNYPLIITASKIGAALATGNSVVLKPASQSPLTALRLAELAVEA
                     GLPGGVFNVVPGPGAVVGPALARHPGVDKITFTGSTEVGKSLLRAVGESDVKAITLEL
                     GGKSPQVVLADVGDLEEAASAIGWGIFYNGGQTCNAGSRLVVHRSISEELVDRIAALG
                     RKLAPAEPLDPATRLGAIVDRRQLEKVLGYVALGQKEGARVVVGGHRARQESGGFFVT
                     PTILDGVSNDWRVAREEIFGPVLTVTEFDDEAEAIRIANDTPFGLAAGIWTRDVKRAH
                     RLARAIRAGTVWVNTFDTADITVPFGGFKQSGFGRDKGIHALDGYTQLKTTWMDLSGD
                     "
     gene            complement(10865..11974)
                     /locus_tag="VHM48_02135"
     CDS             complement(10865..11974)
                     /locus_tag="VHM48_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX2754231.1"
                     /translation="MRISAIRIERLRVPLDPPFPAAWDPVPRTVFPVTIVRVETDEGI
                     VGIGSGDTMDGFAVFEHLFVGQDPLAIARHVRALETIDFHAGRYWPLEVALWDIAGQV
                     AGLPVATLFGGAADGIPAYASCGMLLPAAARAEATLRLRDEGFRAVKIRVDPRRLEEG
                     IAAVAATRAAVGDTMAIMVDINQGWRMAGDVSVSLDFVGARRIAARLAELDVLWLEEP
                     LAGTDLRGLAALRASAPGIRIAGGEMTRTFAETLAALDADAFDVYQPDAVLAAGMLRT
                     RTVGELALARNRWFTPHTWTNGIGLLANLHVAAGVGGGPFLEYPYDPPGWTPERRDAF
                     LAEPVRPGVDGVLRVPTAPGIGAVLDEAAIRRYAA"
BASE COUNT         1809 a         4282 c         4227 g         1738 t
ORIGIN      
        1 gtgaaggggt cgggcacaga cccggtgcgg gcggcgatga acgcccggac gtgcggcagg
       61 caggccccgc cctggcacgg cccgaggccg gcccacgacg cccgcttgag cagctcgaga
      121 ttcgagtagc ccttggcgaa cgcggtctcg aggtcgtcga ccgtcgtgcc catgcacggg
      181 cagacgacgc ccgcggtcgg cgctggtggg agcgggtgat cgtctgcggc cgagccgacg
      241 acgcggacgg cgtcgtcgcg cgacatgcgg gcgaggacgt cgcgtggcgc ccggccgagg
      301 tcgaccacca cggtgtcgca ctcggtcgtc atctcggacc cgtcgggcgc ccgggtgacg
      361 accgcgctga cgcggccacg atcgtcgccc tcgaaccgga tgagctcgcg cccgacgcgc
      421 tcgacacgcc cgagatcgac gccggcggcc cccagtcgtt cggctgcctt cggcgtcagg
      481 attccggcca gatcggagcc ctcgcagacc ggttggagct cggccgcccc ggtcgcgacg
      541 acgacctcgt ccgcgcggag atgcgtcatc cggcccggtt ctcgaacgac gacggacgga
      601 cccggataga tcgcgacgac ttcctggccg gcctgggcgt cgaggaacac gacggcccgg
      661 ccggtcgaac gggcggcttc accggcggca ttcccggcct cggcctgacc ggcgatcacg
      721 aggtcgacgt cggcccgccg gaccggaatt cgttcgccaa cgggtggccg ggtgaggtcg
      781 ccgagttcga cggacgggag cgggggcttc ccgctgccca gctgccgctc gacgaccatt
      841 ccgggttgtg gcgggacttg gcaggtccgg acgtaggcga ccccgtcgac cgcggcgagg
      901 cagttgccgc agtcgccggc aaggcagatc gcaccgccgc gtccggggac ctcgcccgcc
      961 cggatgatgg cgatcgcaac ggagtcgccc tcggcgaact cgatcggtcg cccgtcgacg
     1021 acgatccgac cgccggcccg gttccggccc ttggtcacgc cacgatctcg aacgcgcggt
     1081 cccgtctcgg gccgaatcgg tagatcgcga agtcgctgtc gaaggtcgcg acacgccgca
     1141 cgccgagccg ctccatcaca gcgaagctgg tccggtcgac gatggagaaa tcctggtctg
     1201 gatatccgag tccgatcgcc caagcagcct cgacatcgcg cggctcgatg tactcgaact
     1261 cggcgagtcc ggcccggatc gtctcgaaga agcgttcggc gaccggtcgg ccgagtcgct
     1321 tgcgcagcag ccgccaggtc tcgaacagaa cgtgatcggt ggtcaggaga tcgcccgatt
     1381 cgccggatag cgtccgcttg gcgcgctcat gatcgatgtc gccggcgtcg atggcggcat
     1441 agaacatcga cgtgtcgacg aagacgctca cgggtcagcg ttcgcgcttc gtcttcaggt
     1501 agtcggcctc gaccgcctcg tcgagatact cgtccttgtg cttggcgacg ttcgatccac
     1561 ccgagttgcc caagccgaag atcgcgctga tgtccggctt cgggcgatcc ggctggtcga
     1621 gcgcctggtc cacggaacgc cggacgaact ccgccaggga gatcccgagc tcgtccgcgc
     1681 gacgcttcgc acgccgccgc attccggtct ccatcgtgac ttgcagtcgt gtcatcatca
     1741 tgatggcgcc atcataccat caccgacctc actggcgcat ccggaggccc tgcgggtcga
     1801 acaaaggttc gggctggatc cgggcggggc gccgccggcc gatgatctcg atttcgtagc
     1861 cctggccgcc cggaccgtcc ggcgtcgcga gatcggttgg gacgtagccg agcgcgatcg
     1921 actgcttgac gtagtgccca tacccgccgg acgtgatcca gccgacgacc gtgccgtcat
     1981 gccacaccgg ctcgtcgccg atgacgtcgg ccgggtcgtc cggctgcggg tcgacgacga
     2041 aggtcacgag gcgtcgcttg ggcccgcccg ccgccatctc ggcttcgtgc gccgcccgcc
     2101 cgacgaagtc gtggtcgagc cggaggtagc gcgtgatccc gccctccgcc ggcgtgtaga
     2161 tcgggcggta ctcgcggaac caggtcccgt agctcttctc gagccggagc gacatcagcg
     2221 cccgcatccc gaacagccgt aggtcgtgag gcgcaccggc ctcgacgatc cggtcgaaca
     2281 gcgcccgctg gtgctcgggc gcgacccaca gctcgtagcc gaggtcgccg gagtagttga
     2341 tccgggcgac gagggcgggc tgcatgccga ggtcgacgcg ccggaacgtc atgaacggga
     2401 acgcggcggt cgacaagtcg aggtcgtgtg cgacggcggc gagaacgtcg cgcgacttcg
     2461 gcccggtcag cgacagcccg acgagaccga ggcccaggac ctcgaaccgg accgatccgt
     2521 cggcgggcag gtggtggatg aaccagcgcg cgtgatgggt ctccgccggc agcgagccga
     2581 acaggaagaa ctcgtccgcc tcggacgcgc gggcgaccgt gaactcgccg acgatccgcc
     2641 cggcatcgtt gagcatcgcg gtgagcgtga tccggcccgg cgcgggcatc cgggcggtca
     2701 gcagcgagga caaccacgcc tccgcacccg gccccgtcac ccggtacttc gcgtagttcg
     2761 agatctcggt catgccgacc cgttcgcgga ccgcggcgac ctcctcggcc acgcaatccc
     2821 aggcgttcga gcgccggaac gtgacgtcct ccttcggctc gagcccggcc ttctggaacc
     2881 agagcgcgtg ctcgagcccg gccgtcgcac cccagaccgc gttcgccgcg gtcagccggt
     2941 cgtggatcgg agtggtcaag agcggccggg ccgccggcaa ctcctcgttc gggaacgtga
     3001 tccggaaccg ccggcggtag ttctcctgga ccttcgcgtt cgtgtaggcc agcgtcgcgt
     3061 agtcgccgta gcgcgcgacg tccatcgccc agatatccat gccggagtcg ccggcatcgc
     3121 cagcggtcat ccacgccgac agcgccaacc cgacgccgcc gccctggctg aggcccgcca
     3181 tcacgccaca ggcgacccag tagccgcgca ggccccgcac gggcccgacg agcggattgc
     3241 cgtccggcga gaacgtgaac ggcccgttga cgaccttccg gatgccggtc gtgcccatcg
     3301 ccggatagtg cttgaaggcg acctggagct cgggcgtgat ccggtcgagg tcgggcttca
     3361 ggagctgcgg cccgaagtcc cagggcgtct ccttcgggga ccagggcacg gccgcctgct
     3421 cgtacgtgcc gaggagcatc ccgccgcctt cctggcggat gtagatctcg ccggcgaagt
     3481 cgagcgccat cggcatctcc ttgccagtct tcgcgacgtt cgcggcgacc tcgggcatgt
     3541 cgacagtcag gaggtacatg tgctccatgg cgaggaccgg cagctcgatg ccgaccagcc
     3601 ggccgacctc gcgcgcccac aggccgccgg cgttgacgag gtgctcggcg tggatcgggt
     3661 cgccgttctc gatatggacg tcccaggtgc tgtcgggccg ctgtgagagg ccagtcacca
     3721 tcgtgtgctg gcggacctcg gcgccgagaa gctgggcgca cttcacgtag gcccgcgtga
     3781 cgccgctcgg gtcgacgtga ccctcgactg ggtcgtacaa ggcgccgacg aagtactgcg
     3841 ggtcgagcag cgggaaaacg acctgcgctt ccttcggagt gatcagctcg aggtccatcc
     3901 cgaggtagcg gccgcgcgcc tgggccatcc gcaggaagtc catccggacc tccgtgtcgg
     3961 cgagcatcag gccgcccggc aggtggatcg agcagttctg gccgctgacc cgctcgatct
     4021 cctcgtacag ctggatcgtg tactgctgga gcttcgagac gttcgggtcg ccattgagcg
     4081 tgtgcatccc gccggccgca tgccaggtcg agccggccgt cagctcgcgc cgctcgagga
     4141 ggatcgcgtc cgtccagccc gccttcgtga ggtggtacag gacgctggcg ccgacgacgc
     4201 ccccaccgat gacgacgacc tgagcagacg acttcactcg accctccacg acattccgga
     4261 cgggcgaagc ctacgcgagg agggccgacg gttgcgtcag gctgcgacga gcgcgacgct
     4321 cgcttcgcga cacgccgccg ccaggcggcg gtcgacggtg gcgagcgcca gacctcgtgt
     4381 cgcggccaga tccagataca cggcgtcgta gacggtcagg tcgtgccgtc gggccagatc
     4441 gacgacgtcc agggccgccg agaggtcgat cggaacgacg tcgacgggaa ggtccgtcag
     4501 aagcgaccga gcgaccgaca acttcgcctc gtcgatccgc ccgcgacgct caccgaaccg
     4561 cagagcgttc gcgacctcca ttggccagtg cgccggcacg agcccgccct cggtcaacag
     4621 ctggcgaaga acgccgtccg tcgcatccga cgactcgtca gcgaagcacc aggcgagggc
     4681 aatcgacgcg tcgaccacga acatcagcgc cggccctcgt cgatcagctc ccggatcgtg
     4741 acatcgccga ggacgttgcc cttccggaac tcacgcatcc gcgccatgaa cgcatcgacg
     4801 tcgatgtcgc ggacaacgcc gggaggaacg aggactgcga ccggcctccc gtgcttcgtg
     4861 atcgtgattc gctcgcccct cgcaacttcg tcgaggagcc gaggcaggtg ggtcttggct
     4921 tcataggcgc cgatcgatcg catgcggtca gactagtcgt agaccagtcg atccgtcaac
     4981 cagaccatgg gccagtgctc aggggcggat gatcggcgtc gacgcccggg tcgttccgtc
     5041 cggtcccagg atgaggaggg tccactcggc gccgccgtcc gtcggatata ccgcaagcgc
     5101 gtcgacggtc ccgtcggagc gaacgatcac cgaccaatat ccggccatcg ggtcgggtag
     5161 gaagaccggc cagccgtccc gaaccctccc gtccggagcg gtcgcgacca gcgacccgcc
     5221 ggagctcgcc gatagcccgc caccttcgcc gacggcgctt tccggcacgt acaaggtgcc
     5281 gtccggcccg acgaccggca gcgtccgcca gacgccgcat ccgatatcca cgccgttgca
     5341 cgatcccatg ggctggagcg gcatcttcag tcgggcgggc ggcacgcctc gcccggccag
     5401 gggcggatcg acgacgaagg cgcgctggcc cgtcttgtcc gagccgatca cgaacacggt
     5461 cccgtcactc gcgacgaccg gcgggatgac cctggcccct gccccggtcc gatcgtcgag
     5521 cgggtcgatc ggcaggccgt ccgacgtcgc ggtcgcgtcc ccgccacctg gatcgatcgt
     5581 catcgtctgg gctctggcgc cgtcacccgt gccgattcgc cgcgtgatga ccagccggcc
     5641 attgggaccg acgacgggat gcgacgcaga cccctcgacc gtcacgggcc agcccggccg
     5701 cgcaccgtcg agatcgatcg cgtcgatgac gctgacgacg ggcacgtcgg acgtcggctg
     5761 gtcgacgatc gcgacgcggt aggcgacacc gtccgcggcg aggcggacgt cgaagttgcc
     5821 agcggcatcc gcgacctcga accgttgacc gacccggacg tctccgttgg cggacacgcc
     5881 gatcagccac cacgcggcgg gctgcgccga tccggtgccg tcggtcgagg cgacctctcg
     5941 ggcgaggacg cgaagctcgt tgccgacgac gaccggcggc aggttgaggt cagccaatgg
     6001 aagctcgacg ggccaaccgg gcaacgcctg tccgtccgcg ccgaaggcga gggcgacgtg
     6061 gcgctgcgga ccttccgatg tcgtgtcgga cgcgcagacc aggcgaatcg agccatcggc
     6121 cgcgaccggg agtccgtgcg gcggcgcgtc ggcgtcgcag ctccagccgt cgaacgcgat
     6181 cggccagccg gggcggggtc tgccgtcccg atcgagcagg ccaaggacgc tggtgcttgc
     6241 tgcatcgggg ccgaggtcgc ccgggtggtc agccaggacc cagacgccgc cgtccgggcc
     6301 ggcggcaaat cgcgcggagc cagccccggc cgggaacgac gatggcttcc cgaccggcgg
     6361 cggcccaatg gacggcgacg cgctcggcga ggtctcgacg gacgggcgcg gggtgcccga
     6421 agtcacggtt gcgcgaggga cagaggacgg ctgcgggttt agctcggaat ggtcgcgaag
     6481 ggtggctccc accgcgaacg cgaccccgac ggttgcggcg aggacgaggg ccgccgcgat
     6541 gagcaggacc gcccgtcgtg gtccgtgcgg tcgaccgatt gcacggcgga cccacgtcgg
     6601 gggctcgagg tccggcaggg acgcgagcga cgaggcgtcg cggcgcatgg ccgacgactc
     6661 gaagcggcac gattcgcagg agtggagatg ctcctcgagc tgcccgcggt cgcggtcgtc
     6721 gaggacgaag tcgatcgccg tcgcggcgag ctcgatggcg tggcgatggt cgatcatcgt
     6781 tcgacctccc gaagcagctc gcgcaaccgg acgcgcgagc gggagaggag ggtccgagtc
     6841 gcgctttccg tccgatcgat ggcctggctg atcgccgccc catcgacacc ctgggcggca
     6901 agcaggaggg ccacccgcgc atccgtcgtc agctgatcga gcgcccgatc gagatcgcct
     6961 cgccgttcgc ggcgaagcac ctcgacatcc gggctcgcgt cacgcggttc catgtagcga
     7021 gttcggagca cgcgatcgac ccgagccacg acctggagat gtcgcccccg gctcatcgcc
     7081 aggttggtgg cagcgcggta cagccacgcc cggggttggt ccggccagcg cccgcgacgc
     7141 tcctcgacga ggagcctcgt gaacacgtcg gccagcagat ccgcggctgc gtccgggtca
     7201 cggaccgtgc gaaccaggaa cccgtagacc tgtgcggata gtcgttcgta ggcgtcgccg
     7261 gtctgggcac cgagcgcgtt cggaacgtcg gacacgagca tcgtggcgac ggccagatcg
     7321 ggatcggcgc cgggttccat cacccactgt gacgcccgac ctggtgaact gtgtcagggg
     7381 aggcccgcac gatcggttgg aaccggggcg cctcagaagt ccgtaggcac cccgccctcg
     7441 gacttgcggc ggtcgacgaa cgcggcgagc gcttccttga tcgcctcgtc catggcgggc
     7501 ggctcgtagg cgttcagcaa ttccttccag atcgcgttgg ccttggccgg cgcctgtggg
     7561 ctgcccgctt cctcccaggt ctcgtagttc cgccagtccg agagcatcgg cttgtggaac
     7621 gcggtccgga accggctctg ggtgtgggcg gcgccgaaga aatgcccgcc cgggccgacc
     7681 tcctccatcg ccgagaacgc gagcgtgtcc tcgtcgacga cgaccgggtc gaggaaggcc
     7741 tcgaccatcc cgagcagctc ggcgtcgagg atcaccttct cgtagccggc gtggaggccg
     7801 ccctccatcc agccggcgcc gtgcatcagg aggttgaccc cgccgctgat cgcgccccac
     7861 agcgagaaga ccgactcgta ggccgcctgg gcatcgacgg cgttggcggc cgacacgttg
     7921 gacgaccgat acggcacgtt gtaccgccgt gccagctggc cgccgatcat cgcggtcttc
     7981 atgtactcgg gcgtcccgaa cgccggcgcg cccgactgca tgtcgacgtt cgaggtgaac
     8041 ccgccataga cgaccggcgc gcccggccgg acgacctggg tcaggaccat cccggccagc
     8101 gcctcggcgt tctgctcggc gagcgcgccc gccagcgtca ccggcgccat cgcgcccgcg
     8161 agcgtgaacg gcgtcatcac gatgacctgg ttgcgggccg acatctcgag gatgccgtgg
     8221 agcatcggcg tgtcgagccg gagcggcgag ctcgagttga tgacggtgaa cagcgacggc
     8281 tcgcggtcga gcgtcgcgtc gtcgatgtcg cgggcgatcc gggccatctc gatcgcgtca
     8341 gtgatccgct gccggccgag gctgtaggcg tggatcggct tgtcggcgag ggtcagcagg
     8401 tcccagagcg cgtcgaggtg gcggatcgag gcgtggatgt cgatcggctc gaccgggtag
     8461 ccggcgaaga aatggaccgg gttgagcatc tggcacagcc ggatgaggtt ctggtagtcg
     8521 gcccggttgc cgatccgccg gccgcgatcg aggtcggcga cgttgggcgc gctcgccact
     8581 gacccgaacg ccgtccagtt gcccccgatc tggagattgt ggtcggggtt cgtcgcatgg
     8641 agcgtgaact ccgacggcgc ggtcttgatc cgctcgagga ccaggtcgcg atcgaaccgg
     8701 acacgctgcg tgcctgcctc gacacgcgcg cccgctgcgc gcaggacgtc ccgggcgccg
     8761 tcgtcgagga agtccatccc gatctcttcg aggacgcgga gcgacgcgtc gtggatcgac
     8821 tcgagctcgt cggccgagac gatctcggtc ggcgcgtagc ggatccgcgg ctgggcccat
     8881 ggccgctgct gcgggagccg cgagcgctcg ccggtcgcgc ggccgccacg ccggcgcggg
     8941 gccacctctc cggtgggtgc ctccgcaggt gctgcgccac cgagcgccgc cgccatcagc
     9001 tcctcgtcag tcatgccgcc tccggccctg acacttgtgc gcgacctgca cgttgcgggg
     9061 ccattcgggg ccgcggccag ggctctcgac gtgcaagacg gggcccggac gggccccagc
     9121 cctgacgagt gagcgccggg cgctcgtttg ccggattctc gggccggtcg atccggctga
     9181 cgtgcgccgg atgctcgtcc gtcatgatcg ggcgcggctc acgactacag gcgtgcactc
     9241 ggcgcacatc tggcacgcca tcgccggccg tggcgccctc gacgttcatg gggcgcactc
     9301 ctgtcagggt ccagggtgcc ccgagagccc tgggagccag ttcagtcacc ggacaggtcc
     9361 atccacgtcg tcttgagctg ggtgtagcca tccagcgcgt ggatcccctt gtcccggccg
     9421 aaccccgact gcttgaagcc gccgaacggg accgtgatgt cggctgtgtc gaaggtgttg
     9481 acccagaccg tccccgcccg gatcgcccgg gccaggcggt gggcccgttt cacgtcgcgc
     9541 gtccagatcc cggcggcaag cccgaatggc gtgtcgttgg cgatccggat ggcctccgcc
     9601 tcgtcgtcga actcggtcac ggtcaggacc ggtccgaaga tctcctcgcg ggcaacccgc
     9661 cagtcgttcg agactccgtc caggatcgtc ggggtgacga agaacccgcc gctctcctgg
     9721 cgcgcccgat ggccgccgac gacgacccgg gcgccctcct tctgaccgag cgcgacgtag
     9781 ccgaggacct tctcgagctg acgccggtcg acgatcgcgc cgaggcgggt cgccgggtcg
     9841 agcggctccg ccggcgcgag cttgcgaccg agcgcggcga tccgatcgac gagctcctcg
     9901 ctgatcgagc gatggacgac gagccgcgac cccgcgttgc aggtctggcc gccgttgtag
     9961 aagatccccc agccgatcgc cgatgctgcc tcctcgaggt cgccgacatc ggccaggacg
    10021 acctgtgggc tcttcccgcc cagctcgagg gtgattgcct tgacgtcact ctcgccgacc
    10081 gcccgaagca gcgacttgcc gacctcggtc gagccggtga acgtgatctt gtcgacgccg
    10141 gggtgacggg cgagcgcggg cccgaccacg gcgcccggcc ctggaaccac gttgaagacg
    10201 ccgccgggca ggccggcctc gacggccagc tccgccagcc ggagcgcggt caacggcgat
    10261 tgggacgccg gcttcaggac gaccgagttg ccggtggcca gcgccgcccc gatcttcgac
    10321 gccgtgatga tcagcgggta gttccagggc acgatcgcgg ccacgacccc gatcggctcg
    10381 cgggtgacga ggctcagggc gtccggcccc gtcggcccga cctcgccgta gaccttgtcg
    10441 atcgtctccg cgtaccactg gatcgtcgcc gcggccgatg gcacgtcgac cgcgagcgtg
    10501 tcgcggatcg gcttgccgac gtcgagcgac tcgaggaggg cgagctcatc gagatggtcg
    10561 cgaatcgtgt cggcgaaccg gagcagcacc ttcttgcgct cgagcggctt ccggtccgcc
    10621 cagcgccggt cgtcgaagga atggcgggcg gccgccaccg cccggtcgac atcctcggca
    10681 ccaccctcgg cgacctgggc gatcgtcgag ccatcgcggc cggcgatgtc ctcgaacgtg
    10741 cggccggagg ccgcgggcac gaaccgcccg tcgatgaacg catcggttcg cggtgtgatc
    10801 gccgcggccc gttcgatcca ctcggcggtc gtcgtccgat gccgctcgcg ttcggcggtg
    10861 ccggtcacgc cgcgtaccgc cggatcgccg cctcgtccag gacggccccg atcccgggcg
    10921 ccgtcggcac tcgcaggacc ccgtccacgc ccggccgcac gggttccgcg aggaaggcgt
    10981 cgcggcgctc cggggtccag cccggcgggt cgtacggata ctcgaggaac ggtccgcccc
    11041 cgacacctgc cgcgacgtgg aggttcgcca gcagcccgat cccattggtc cacgtgtgcg
    11101 gcgtgaacca ccggttgcga gccagggcca actccccgac ggtccgcgtc cggagcatcc
    11161 cggccgccag caccgcgtcc ggctggtaga cgtcgaaggc gtcggcgtcg agggcggcga
    11221 gggtttcggc gaacgtccgg gtcatctcgc cgccggcgat cctgatcccc ggagcggagg
    11281 cgcgcagcgc ggcaaggccc ctcaggtccg tgccggcgag cggctcctcc agccagagca
    11341 cgtcgagctc ggcaagacgg gcggcgatcc gccgggcgcc cacgaagtcc aacgacaccg
    11401 acacgtcgcc ggccatccgc cagccctggt tgatgtcgac catgatcgcc atcgtgtcgc
    11461 cgacggcggc ccgggtcgcc gcgacggccg cgatcccctc ctcgagtcgc cgcgggtcga
    11521 cccggatctt gaccgcccgg aacccctcat cgcgaaggcg aagcgtcgcc tcggcacgcg
    11581 ccgccgccgg caggagcatc ccgcacgacg catacgccgg gatcccgtcc gcggccccgc
    11641 cgaacagcgt cgcgaccggc aagcccgcga cctggccggc gatgtcccag agcgcgacct
    11701 cgagcggcca gtaccggccg gcgtggaagt cgatcgtctc gagtgcccgg acgtgtcgag
    11761 cgatcgcgag cgggtcctgc ccgacgaaca ggtgctcgaa caccgcgaac ccgtccatcg
    11821 tgtcgcccga tccgatcccg acaatgccct cgtcggtctc gacccggacg atcgtcaccg
    11881 ggaacacggt tcgcgggacg gggtcccacg cggccgggaa cggcgggtcg agcggcaccc
    11941 tcagccgttc gatccggatg gcggagatcc tcatgtggga ggcagtatcg ctgacgatcc
    12001 gcgatgcggt actgtcggcg ctcacccgcg aaaccacgtc cgacccgccc gccagg
//
LOCUS       DATAIW010000064         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_180053, whole genome shotgun sequence.
ACCESSION   DATAIW010000064 DATAIW010000000
VERSION     DATAIW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180053"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VHM48_02140"
     CDS             complement(<1..455)
                     /locus_tag="VHM48_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754232.1"
                     /translation="MTHRLRLRRLAGLLLGASALSVALIAPSGALAATPTSTATDGAT
                     ATACAGGAWPYAVQGAPVLLAGSAAGDHLWHDATGWHLRVTHPGTYAAGFSGTIRANR
                     PLHVAGFRLEGGDTFTVSADGLSVSYRFVNHGRLDGLDFTTECATRLGV"
     gene            689..1540
                     /locus_tag="VHM48_02145"
     CDS             689..1540
                     /locus_tag="VHM48_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2754233.1"
                     /translation="MARNVRGALLQATWTGDKESMIQKHEQAAHDASRQGAQVMLFQE
                     LFYGPYFCQVQDPQYYSYTELIPDGPTTKRMEDLAKQTGMVLVVPMYEEDEKASGIYY
                     NTAAVIDADGKYLGKYRKTHIPHVKGFWEKFYFRPGNMGYPVFETAVGKVGVYICYDR
                     HFPEGARALGLNGAEMVFIPSATSRGLSEYLWRIEQVSHAVANGYYVGTINRVGIEKD
                     YGDDDFYGQSYFVNPRGQFIGELGSAYDEELIVRDMDLDLITEVRQTWQFYRDRRPDA
                     YNDLVRP"
     gene            1566..2558
                     /locus_tag="VHM48_02150"
     CDS             1566..2558
                     /locus_tag="VHM48_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2754234.1"
                     /translation="MDFGFTLKPDSSIERTIALTRQAESAGFVAGWLFDSHVLWRDPY
                     PLLTLMAQATERMRLGTCVTNPGTREPSVTASTLATLDELSGGRMDLGIGRGDSARRI
                     LGKPPTTMATLEEAIRVIRALVAGETIAYEGTDLHFPWTSGWELPVWVAGYGPMALAL
                     TGRVADGVILQLADPDLIAWFVGQVREAEAAAGRPVGSVRIQAAAPAHVGELDGCRKR
                     TRWFPALVSNHVVDLVNKYPREQLPEALTGYIHDREGYDYLHHAEVGSSNAGFVGDEV
                     TDRFCVLGSVDDHLAKLHRLAEVGVDQFNIYLMNGDEEDVLEVYGREVIPAMGG"
     gene            2734..3015
                     /locus_tag="VHM48_02155"
     CDS             2734..3015
                     /locus_tag="VHM48_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754235.1"
                     /translation="MSRLSLTLHRHDPYWDMDGAGARHQRTKQRVVRIAIWFTLVAAL
                     AYVATRLPAVDPDYLISGAGRPILAGALLTLLGAAALLALAHVRHVSRD"
     gene            3313..4254
                     /locus_tag="VHM48_02160"
     CDS             3313..4254
                     /locus_tag="VHM48_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754236.1"
                     /translation="MRLIAAHGRGPMFRRDGRPRDRIARGAIGIGLIGLIGLLGSVGV
                     GTVVAATTVNLDQWASTDLAWQNGNLNGNNSRYPEGGIVPFRLAVEGLTVGEHVIHIN
                     YDVTAGGHKAYDFLATWNVTNASGKACAASGGGISSMCPSLPASSSAALPNDPFVMDG
                     LSVHGAQVYSGAPRRLTIWGGTIVSISAPTHSGPTSGNSSGDILVRFRATGRAVLLAW
                     GGHLGQSAYWNRLNGGPSDGAGMVSGSPWHMRTLQLDGSGNKNQDRSISPSAIVGELP
                     PFALAPPTPTPRPTAPPAAPGRTPGNGGGGAGRAGAR"
BASE COUNT          675 a         1546 c         1510 g          627 t
ORIGIN      
        1 ctgaccccga gccgggttgc gcactcggtc gtgaagtcga ggccgtcgag ccggccgtgg
       61 ttgacgaacc ggtacgagac gctgagaccg tcggcgctga cggtgaaggt gtcgccgccc
      121 tcgagcctga agcccgcgac gtggagcggg cgattggccc ggatcgtgcc gctgaacccg
      181 gcggcgtagg tccccgggtg ggtcacacgg aggtgccaac cggtcgcgtc atgccagaga
      241 tggtcgccgg ccgccgatcc cgccagcaga accggcgctc cctggaccgc gtacggccag
      301 gcgccccccg cgcaggcggt ggcggtggca ccatccgtgg ctgtcgacgt cggtgtcgcc
      361 gcgagcgcgc cgctgggcgc gatgagggcg accgacagag cgctcgcccc gaggaggagg
      421 cctgccagac ggcggagtcg gaggcggtgg gtcatggttc gatctcccgc tgtgtctcgc
      481 ccgttcgttc gggctcatcg cggtcgaccg tagtcctgca ccgggaggcg tgccgccgtt
      541 cgatgggcgt caacggtaag gagcgggtaa gacggaccga ccgccacggc ccgcgctggg
      601 acggacgcgg cggccgtccc gctccgcggt cgtcgttgta cgctcatggc cttccgaccg
      661 catctgtcac gttcgagtga gggacgacat ggcgcgcaac gttcgaggcg cactcctcca
      721 ggcgacctgg accggcgaca aggaatcgat gatccagaag cacgagcagg cggcccacga
      781 cgcgagcagg caaggcgccc aggtcatgct cttccaggag ctgttctacg gtccctactt
      841 ctgccaggtc caggatcccc agtactacag ctacaccgag ctgatcccgg acggcccgac
      901 gacgaagcgg atggaggacc tcgcgaagca gacggggatg gtcctcgtcg tgccgatgta
      961 cgaggaggat gagaaggcgt cggggatcta ctacaacacc gccgcggtca tcgatgctga
     1021 cggcaagtac ctcggcaagt accgcaagac ccacattccc cacgtcaagg gcttctggga
     1081 gaagttctat ttccgaccgg gcaacatggg ctatccggtg ttcgagacgg cggtcggcaa
     1141 ggtcggggtc tacatctgct acgaccgtca cttcccggag ggcgcccggg cgctcgggct
     1201 caacggcgcc gagatggtct tcatcccgag tgcgacgtcg cgcggcctgt cggaatacct
     1261 gtggcggatc gagcaggtca gccacgcggt cgccaacggc tactacgtcg ggacgatcaa
     1321 tcgcgtcggg atcgagaagg actacggcga cgacgacttc tacggccaga gctacttcgt
     1381 caacccacgc gggcagttca tcggcgagct cggctcggct tacgacgagg agctgatcgt
     1441 ccgcgacatg gacctcgacc tgatcaccga ggtccgccag acctggcagt tctaccggga
     1501 ccgtcggccc gacgcgtaca acgacctggt caggccttag cgcaggcacc acccgaggcg
     1561 ccgagatgga cttcggattc acgctcaagc ccgacagctc catcgagcgg acgattgccc
     1621 tgacccgcca ggcggagtcg gccgggttcg tcgccggctg gctcttcgac tcgcacgtcc
     1681 tgtggcgcga tccgtacccg ctcctgacgc tcatggccca ggccaccgag cggatgcgcc
     1741 tcgggacctg cgtgacgaac ccgggcacgc gcgaaccgag cgtcacggcc tccaccctgg
     1801 ccaccctcga cgagctcagc ggcggccgga tggacctcgg catcgggcgc ggcgactcgg
     1861 cccggcggat cctcggcaag ccgccgacga cgatggcgac cctcgaggag gcgatccggg
     1921 tgatccgggc gctcgtcgcc ggcgagacga tcgcgtacga gggcacggat ctccatttcc
     1981 cgtggacgag cggctgggag ctgccggtct gggtcgccgg ctacggaccg atggccctcg
     2041 cgctgaccgg tcgggtcgcc gacggggtga tcctccagct cgccgacccg gacctgatcg
     2101 cgtggttcgt cggccaggtc cgcgaggcgg aggcggccgc cggccggccg gtgggctcgg
     2161 tccggatcca ggccgccgcc ccggcccacg tcggcgagct cgacggctgc cgcaagcgga
     2221 cgcgctggtt cccggccctt gtcagcaacc acgtcgtcga cctggtcaac aagtacccgc
     2281 gcgaacagct ccccgaggcg ctcaccggct acatccacga ccgcgagggc tacgactacc
     2341 tccaccacgc cgaggtcggc tcgtcgaacg cgggcttcgt cggcgacgag gtcacggatc
     2401 gcttctgcgt cctcggctcg gtcgacgatc acctggccaa gctccatcgc ctggccgagg
     2461 tcggtgtcga ccagttcaac atctacctga tgaacggcga cgaggaagac gtcctcgagg
     2521 tctacggccg ggaggtcatt ccagcgatgg gtggctgacc cggacgcgac cggatccctg
     2581 acccccggtg cgccaacgca ccgagccggc ctgaccgggt tcggtctggt gcagttgcag
     2641 gagggtccct ggccggattg ggggactggc ggaagcgttc ccggccggtc acgatcgacg
     2701 agccccaagt cacaccgccg gaggacccag cccgtgtctc gcctgtcgct cacgctccac
     2761 cgccacgatc cgtactggga catggacgga gccggcgccc ggcatcagcg gacgaagcaa
     2821 cgcgtcgtcc gcatcgccat ctggttcacg ctcgtcgcgg cgctcgccta tgtcgcgacg
     2881 cgtctgccgg cggtggatcc cgactatctc atcagcggtg ccggccggcc gatcctggcg
     2941 ggtgccctcc tgacgctcct cggagctgcc gcgcttctcg ccctcgccca cgtccggcac
     3001 gtcagccgcg actgagctcc caccgagcgg acccgcgcgg ggcgcggcgc cgcccagatg
     3061 gcatgtcccg ggccgagtcc ttgcgtcgga gtcaacgcga cggcgcttcg ttgtgcctga
     3121 gcaacacgtc gggccgagcg cggcccgccg attcggtgta accccgcacc gtaatcgcgt
     3181 ccctcgacct gcccgaggtc cagtcgagtg cggtccggac gggtcgatag cccgcccgtc
     3241 accctcgaca acgggacccc tgccgagacg acactggcgc ccgagagcgc agtgtcaccg
     3301 gagggacctg tcgtgcgatt gatcgctgcc cacggccgcg gcccgatgtt ccgcagggac
     3361 ggacgcccgc gcgaccgcat cgcccgtggc gcgatcggga tcggcctgat cgggctgatc
     3421 gggctgctcg gctcggtcgg cgtcgggacg gtggtggcgg cgacgaccgt caacctcgac
     3481 cagtgggcga gcaccgacct ggcgtggcag aacggcaacc tcaacggcaa caactcgcgc
     3541 taccccgagg gcggcatcgt tccattccgg ctggccgtgg aggggttgac cgtcggcgag
     3601 cacgtgatcc acatcaacta cgacgtcacc gcgggcggcc acaaggccta cgacttcctc
     3661 gcgacctgga acgtcacgaa cgcctccggc aaggcctgcg cggcgtccgg tggcgggatc
     3721 tcatcgatgt gcccgagcct gccggcttcc tcgtccgccg cgttgcccaa cgatccgttc
     3781 gtgatggacg gcctgtcggt gcacggggcg caggtctact ccggcgcacc gcggcggctg
     3841 accatctggg gcgggacgat cgtgtcgatc tcggcaccga cccacagcgg cccgacgagc
     3901 ggcaacagca gcggcgacat cctcgtccgg ttccgggcga ccggcagggc cgtgctcctc
     3961 gcgtggggcg ggcatctcgg ccagtcggcc tactggaacc ggctcaacgg gggaccgagc
     4021 gacggcgccg gcatggtgtc cggctcgccc tggcacatgc gcaccctcca gctcgatggc
     4081 tccggcaaca agaaccagga tcgctcgatc tcgccgagcg cgatcgtcgg cgagctgccc
     4141 ccgttcgccc tcgcgccgcc aaccccgacg cccaggccga ccgcaccgcc ggccgctccc
     4201 gggcgcacac cgggcaacgg cggcggcggc gcagggcggg caggagcgcg atgaggccga
     4261 ggcccgtcgt cgccaggatc gccgctagac cgccgtcgcc cgaacgacca ggggacggaa
     4321 gcgggttcgt cgacgtcggt gggatcgtga tccgcggg
//
LOCUS       DATAIW010000065         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_32682, whole genome shotgun sequence.
ACCESSION   DATAIW010000065 DATAIW010000000
VERSION     DATAIW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32682"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..2136
                     /locus_tag="VHM48_02165"
     CDS             364..2136
                     /locus_tag="VHM48_02165"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX2754237.1"
                     /translation="MRMTRLLRRRVLAVGLGSAVLIGLGAPAGQPASAATYPSWCRYS
                     SRPVDRAAELLANRYLLSPHRIVTLPADPTWRENPLHDANWQFQYQAMRYVLDLFAAW
                     NSTGVDAYRARALFLLHDWYVSNPRANPRSAYSWNDASTAFRAVVYACAADLTPMTTW
                     LRNALLLHGRTLADPAFYVGIGNHALSQSIGLLEVGRVLGRRDWMTLARDRLNRLVLS
                     SVDAQGVTNEQAAFYQTYNYVHYRLARSRLLAVGLSPGSGFARVDLMPRFLAQTALPN
                     GEYEMIGDTEAVAIPSYPGTWTEFIATKGASGPTPPLVAAYADGYQFARTGWGTNRAF
                     ADETSIAIRWGPAPRTAHGHPDGTSVTLYAWGSRLLVGPGKYTFNSGSWRSFFTSRRA
                     NSVVTVDGLSWNRAASTTREGRVVTSTLVDVRLKTQGFAGVTQTRRITWSRRLGYMLV
                     DDRAQSLVRHTYRQLWHLVNGANPRIGVSTVWTQRPRGNVLIRQLTGSPTLRVVTGAT
                     DPIQGWISYRYGQRVAAPVVQAIRTGTSVRYLTLIAPSEGAPDARVTNLRLTAGGYAV
                     TITIGGRSERVIASGSAVSITPLN"
     gene            2225..>2794
                     /locus_tag="VHM48_02170"
     CDS             2225..>2794
                     /locus_tag="VHM48_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2754238.1"
                     /translation="MTDDPGDSRLIDPSAPQYNATLVRRVDETDDLAYVWVKFDGDPT
                     PFEPGQYMTIGVVADGKLWQRPYSVASAPRVAGDEGYEFYVRLVPIERFTSLLWRLPI
                     GHGMRMIGPKGKFLLEPEDDRTHVYVSTGTGVAPFIAMVRETMAAGSPRRTIVLHGCS
                     FEDELGYRPILEGWEQDGTYPLAYIPTISR"
BASE COUNT          404 a         1003 c          966 g          421 t
ORIGIN      
        1 tagatgtacc gccgcgcggc acgatgggga cggtcggtcg gggatgcggc ggtcgtggcc
       61 atcgggcggc tcctcgggtc atgtggcgtc gcccgtgggc ggcgcacgga gacggttcgt
      121 tgcagatggc aattgtaccc accggctccg accggctcgg gttgcggatc ggtggcggtc
      181 gctgccggga acttccgcgc cgcgccggtc ggtcggttcg gcccgaagtt gatgacgccg
      241 acgccagcca atgggtccat actcgaagcc gacacgtatc gcgaggggat cgatcgtgga
      301 gacggcgctg ggcgagggcg tggatcgggc gctgcgcacg ggcgagggcc cggaagtcgc
      361 gcgatgcgca tgacccggct ccttcgacgc cgcgtgctcg cggtcgggct cggcagcgcg
      421 gtcctcatcg gcctcggcgc accggcgggt caaccggcct cggccgcgac ctatccatca
      481 tggtgtcgct actcgagcag gccggtcgac cgcgcggcgg agctgctcgc gaaccgctac
      541 ctcttgtcgc cgcaccggat cgtgaccctg ccggccgacc cgacatggcg cgagaacccg
      601 ctccacgacg ccaactggca gttccagtac caggccatgc gctacgtcct ggacctgttc
      661 gccgcctgga acagcaccgg cgtcgatgcc taccgggccc gcgccctgtt cctgctccac
      721 gactggtacg tcagcaaccc ccgggcgaac cctcggtcgg cctacagctg gaacgatgcc
      781 tcgaccgcgt tccgggccgt ggtgtacgcc tgcgccgcgg acctgacgcc catgacgacg
      841 tggttgcgga acgcgctcct cctgcacggc cggaccctcg ccgatccggc gttctatgtc
      901 gggatcggga accacgcgct cagccaatcg atcgggctgc tcgaggtcgg acgggtcctc
      961 ggtcgacgcg actggatgac gctcgcgcgg gaccggctca accgcctcgt gctctcgagc
     1021 gtcgacgccc agggcgtcac gaacgagcag gctgccttct accagaccta caactacgtc
     1081 cattaccggc tcgcccggag ccggctgctc gccgtgggcc tgtcgccggg ctcgggcttc
     1141 gcccgcgtcg atctcatgcc ccggttcctg gcccagaccg cgctgccgaa cggcgagtac
     1201 gagatgatcg gcgacacgga ggcggtcgcg atcccctcgt acccgggcac ctggaccgag
     1261 ttcatcgcga cgaagggcgc gagcgggccg acgccaccgc tcgttgccgc ctatgccgac
     1321 ggataccagt tcgcgcgcac cggctggggc acgaaccggg cattcgccga cgagacctcg
     1381 atcgcgatcc gctggggccc cgcgccgcgc acggcgcatg ggcacccgga cggcacctcc
     1441 gtcacgctct atgcgtgggg gtcccggctc ctcgtcgggc ccggcaagta cacgttcaac
     1501 agcggaagct ggcgctcgtt cttcacgagc cggcgggcga acagcgtcgt cacggtcgac
     1561 gggctgagct ggaaccgggc tgccagcacg acccgggagg gccgggtcgt cacgtcgacg
     1621 ctcgtcgacg tccgactcaa gacgcaaggg ttcgccggcg tcacccagac gcggcggatc
     1681 acgtggtcgc ggcgactcgg ctacatgctc gtcgacgatc gggcccagtc gctcgtccgg
     1741 cacacgtacc gccagctgtg gcacctcgtg aacggcgcga atcctcgcat cggggtctcg
     1801 acggtctgga cgcagcggcc ccgcgggaac gtgctgatcc ggcagctgac cggttcgccg
     1861 acgctgcgcg tcgtcaccgg cgcgaccgac ccgatccagg gctggatctc gtatcgctac
     1921 gggcagaggg tcgccgcgcc ggtcgtccag gcgatccgga ccgggacctc ggtccgctat
     1981 ctgaccctga tcgccccgtc cgagggtgct cccgatgccc gggtcacgaa cctgaggctg
     2041 acggcgggcg gatacgcggt gacgatcacg atcggcggcc gatccgagcg cgtgatcgcg
     2101 agcggctcgg cggtgtcgat cacccccctc aactgaccgg cgccggcccg gagctcgggg
     2161 cggaggcgac cgatgcccgg gtaccatcgt ggtcgtccag ccaagcccga ggccaccatc
     2221 cgccatgacc gacgatcccg gcgacagccg cctcatcgac ccgtccgccc cgcagtacaa
     2281 cgcgaccctc gtccggcgcg tcgacgagac cgacgacctc gcctatgtct gggtgaagtt
     2341 cgacggcgat ccgaccccgt tcgagccggg ccagtacatg acgatcggcg tcgtcgcgga
     2401 cgggaagctg tggcagcggc cgtactccgt cgcctcggcg ccccgggtcg cgggtgacga
     2461 aggctacgag ttctatgtcc gcctcgtccc gatcgagcgg ttcacctcgc tgttgtggcg
     2521 gctgccgatc ggccacggga tgcggatgat cgggccgaaa ggcaagttcc tcctcgagcc
     2581 cgaggacgat cggacgcacg tctacgtctc gaccgggacg ggcgtcgcgc cgttcatcgc
     2641 gatggtccgc gagacgatgg ccgccgggtc gccgcgccgg acgatcgtcc tccatggctg
     2701 ctcgttcgag gacgagctcg ggtaccgtcc gatactcgag ggctgggagc aggacggcac
     2761 gtatccgctg gcctacatcc cgacgatctc ccgc
//
LOCUS       DATAIW010000066         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_151819, whole genome shotgun sequence.
ACCESSION   DATAIW010000066 DATAIW010000000
VERSION     DATAIW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151819"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..650
                     /locus_tag="VHM48_02175"
     CDS             99..650
                     /locus_tag="VHM48_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754239.1"
                     /translation="MTRHAPRRILRLVAGLGSDGIARLRNGLADLFTVAPAGRDELLA
                     AFAGTAHGRGGDGRFGLWMRDGGALLTARRAAFEPFLPGGGRALRGLDVVLLGVALER
                     LAGIDPGAVATGGQVSFTKSAADALDAVDTSRDGIDAAFLLEPTPVASIAAVAADGDV
                     MPQKSTYFYPKALTGLVINPLEW"
     gene            691..1569
                     /locus_tag="VHM48_02180"
     CDS             691..1569
                     /locus_tag="VHM48_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2754240.1"
                     /translation="MTESTPEHILARAQVPSANGRPIRKDEIELHDRGIYIESWLPER
                     RSRRKPLLFVHGELAGSWIWERFLGYFASRGWEGHALNLQNHFWSRTVDPTQLTFDSY
                     VEDVVVSLNRLGPTTVVIGHGLGGLLALKAAERAPVGGLVFLSPEPPSALRDAAAVHQ
                     LRDVPDVYGRARIGWSTLPEKLQRDHRDLTIGDVLRIQHLLGQKPHESGRARRSVLAG
                     IAVDPRHVAEIPRLVIGAGLDTVVPEPTSERFAAWLGAEYEPFGAHSHYGLIVGEQSH
                     QQVADAIRGFLEAHRL"
     gene            1665..1741
                     /locus_tag="VHM48_02185"
     tRNA            1665..1741
                     /locus_tag="VHM48_02185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Glu,seq:ctc)
BASE COUNT          240 a          639 c          591 g          273 t
ORIGIN      
        1 acatccgtct ggccggtccc gcgaccggga gcgcaacttt tcggggggcc gggacgtcga
       61 tgctgcgacc gcaagtcgtt cccctacctg gagtccccgt gacccgccac gcgccacgcc
      121 ggatccttcg cctcgtcgct gggctcgggt cggacggcat tgcccgcctc cgcaacggcc
      181 tcgccgacct gttcacggtc gcgccggccg gacgcgacga gctgctggcg gcattcgcag
      241 gcacggcaca cggccgcggt ggcgacggcc ggttcgggct ctggatgcgt gacggcggcg
      301 cgttgctgac cgctcggcgg gccgcgttcg agcccttcct cccgggcggc gggcgggcgc
      361 tgcgcgggct cgacgtcgtc ctgctcggcg tggccctcga acggctggcc gggatcgatc
      421 caggtgcggt cgcgaccggc gggcaggtgt ccttcaccaa gtcggccgcc gacgcgctcg
      481 atgcggtgga cacgagccgc gacggaatcg acgccgcgtt cctgctcgag ccgacgccgg
      541 tcgcgtcgat cgccgccgtc gcggccgacg gcgatgtcat gccccagaag tcgacctact
      601 tctatccgaa ggccctgacc ggcctcgtca tcaacccgct cgaatggtga accgaccgat
      661 ggatgtcagc gcaaccccgg gccgccagcg atgaccgaat cgacgccgga gcacatcctc
      721 gcccgggccc aggtcccgtc ggccaacggc cggccgatcc gcaaggacga gatcgagctc
      781 catgatcgcg gcatctacat cgaatcgtgg cttccggagc ggcgatcccg gcggaagccc
      841 ctcctgttcg tccacggcga gctggccggc tcatggatct gggagcggtt cctcggctac
      901 ttcgcatcgc gcggctggga gggtcatgcc ctcaacctgc agaaccattt ctggtcccgg
      961 acggtcgatc cgacccagct gacgttcgac tcgtacgtcg aggatgtcgt cgtcagcctc
     1021 aaccggctcg gaccgacgac cgtcgtgatc ggccacgggt tgggcggact gctggccctc
     1081 aaggcagccg agcgcgcccc ggtcggcgga ctcgtctttc tctcgcccga gccgccgtct
     1141 gccctccggg atgctgcagc cgtgcatcag ctgcgcgacg tccccgacgt ctacggccgg
     1201 gcgcggatcg gctggtcgac gctgcccgag aagctccagc gcgatcaccg cgacctcacg
     1261 atcggcgatg tcctgcggat ccagcacctc ctcgggcaga agccgcacga gagcggtcgc
     1321 gcccgacgat cggtcctggc ggggatcgcc gtcgatccgc gccacgttgc cgagatcccg
     1381 cgtctcgtga tcggggccgg cctcgacacc gtggtcccgg agccgacgtc ggagcggttc
     1441 gcggcctggc tcggcgccga gtacgagccg ttcggggcac attcgcacta cggcttgatc
     1501 gtcggcgagc agagccacca gcaggtcgcc gacgccatcc gcgggtttct cgaggcgcac
     1561 cgcctctgaa gcagcgtggg tccgccgcgg cgggtggcga cgatcgcggt ccgttgctat
     1621 catcccgatc cggccgcgac atcgatccgt cgccgccgtt cggagccgca ttcgtctaga
     1681 ggcccaggac accgccctct caaggcggag atcaccggtt cgaatccggt atgcggtacc
     1741 acc
//
LOCUS       DATAIW010000067         7355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_2669, whole genome shotgun sequence.
ACCESSION   DATAIW010000067 DATAIW010000000
VERSION     DATAIW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..2935
                     /locus_tag="VHM48_02190"
     CDS             80..2935
                     /locus_tag="VHM48_02190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX2754241.1"
                     /translation="MATTAASPTDRPHRAARRYIYAWGEGTAEGNGGMKDLLGGKGAG
                     LAEMTIAGLPTPPGFTITTEACNDYFAAGEQLPDGLWEDVLDAMREVERRSGKGFGDA
                     ANPLLVSVRSGAKFSMPGMMDTVLNLGLNEETLQGLVALTGNERFGWDAYRRFIEGFG
                     RIVMGVEGDRFNERLDARKERHGADAKDTDLDVADLKTLVDEYKSIVREDTGRDFPTD
                     PNEQLDLAIKAVFASWFGKRANDYRNSQKIAHNLGTAVNIVSMVYGNMGDDSGTGVAF
                     TRNTIDGRRELQGEFLPNAQGEDVVAGIRTPIEIAEMKNRWPEVYAEFDRIGQRLENH
                     YRDVQDMEFTIERGKLYMLQTRSAKRTAASAVKVAVDMVAEGMITKEEAVGRIETAQV
                     DQLLRAQFDPKARTSAQRIAKGLNASPGAAVGKAVFDADVAVEWVGRGEKVVLVRVET
                     SPDDFHGMAVAQGILTARGGATSHAAVVARQIGKPCVAGCAELLVDYGSKTARSSDSG
                     LSFGEGDWISVDGSTGEVFVGAIPTVEARFEDQPELQTILGWADGFSRMAVWTNADKP
                     EEAAQARSYGARGIGLCRTEHMFREGERLDIVRGAILVAGVATRAKARRDAKAAAEAK
                     AAKGDDISTDEKTARAVELTADEQEAIATFDAAMAKLEVLQQGDFEGIFKAMDGLPVV
                     VRLIDPPLHEFLPNLEEQLVKVTRAEMDGGASDEDKELLATIRSLHEQNPMLGLRGCR
                     LGLMIPDFVKIQTRAILNAEIAVKKGGGHPIAKIMIPLVGHVNELAFTRALLEKEASA
                     VREAAGGVEVDYKFGTMIEVPRGALTADEIATEADFFSFGTNDLTQMTFGFSRDDAEG
                     GFLLKYVEDGILPVNPFQTLDDAVAGLMKTAVDKGRSTKPGLELGICGEHGGDPDSIH
                     KCEKIGLDYVSCSPFRVPVARLAAAQAAMAGAAERDK"
     gene            complement(3059..4810)
                     /locus_tag="VHM48_02195"
     CDS             complement(3059..4810)
                     /locus_tag="VHM48_02195"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX2754242.1"
                     /translation="MRPLPAPFEEIAAGRPAWIGLRSSALLGARLLTKDLAFPPAERV
                     AFGLDGLLPDRVLTIEEQVELELEHLRRKADPLERYIGLAALQDRNATLYYRLLTEHA
                     EEFLPIVYTPTVGRACQEFSHIIRRTRGLWITPADIDRIPDLLRNSPYDDVRLIVVTD
                     NERILGLGDQGAGGMAIPIGKLALYTAASGIHPALTLPVSLDVGTDNPLLLADPLYLG
                     HRGPRLRGAEYDALVEAFVAGVETTWPDCVIQWEDFKQHNALRILDRYRDRFCSFNDD
                     IQGTAAVVLGGILAAVRSLGGRLVDQRVVIAGAGAAGIGIARLLRLAMIEDGATPDEV
                     TVAIAMVDTHGLVHDGRADLDDDKREVARPFARLTAEGFGDFSTTPTLDATVRALRPT
                     ILLGATGTPGTFDEALISDVAAGVERPIVMPLSNPTTATEAIPADIFRWSGGRAIVAT
                     GSPFAAVEYDGRRVEIGQANNVFVFPGLGLGAIVAEAARMPDELFLIAARTLATCVSN
                     ERLAAGVIYPSVSELRSVTRAIATAVARTVVRLGIAGCDQTSDEDVDAAVDRAMWWPD
                     YVPYLPEDEVLADAGSY"
     gene            complement(5207..6337)
                     /locus_tag="VHM48_02200"
     CDS             complement(5207..6337)
                     /locus_tag="VHM48_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2754243.1"
                     /translation="MAEPVEIRAAVVDRPGVAVRIETLLLDPPGPGEVRVRMAAAGVC
                     HSDLHVRDGEWQREGPIVLGHEGSAVVEALGEGVAAAFPGLKPGVLVALAWLIPCGRC
                     RACRAGRVWSCSESPSYTHRMPDGAAALHRPAGGGDVLTYCAIGTFADRQNVPAAAAI
                     PMPDGTPPEVAALIGCCVTTGVGAATKTAEVQPGSSVVVIGLGGVGLSCVMGAALAGA
                     GRIVAVDRVAAKLELARAVGATDGLLAGSDAAETLAAIRAATDGGPDTCFEAIGLPAT
                     IETAIACLPTGGTAVLVGMTPFGVRASFEPFPFVDGGRRILGSNYGSADPAVDFPRYA
                     ALHLAGRLPAERLVTARIGLDGIEAAFAAMRRGDGVRSIIAF"
     gene            6389..7294
                     /locus_tag="VHM48_02205"
     CDS             6389..7294
                     /locus_tag="VHM48_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX2754244.1"
                     /translation="MPHDVPEDWRSPLPPGFRWPDGKRAAACLTFDVDAESPILFEHP
                     ESAAWLDVMTHQAYGARTGIARLLRLLDRRRVRATFFIPGYSAERWPEICRAIRDAGH
                     EIAHHGYLHEGAHGATPADEESRLLRGLAALDEVLEIRPTGYRGPNWEMTYETPALLA
                     RHGFTYDSGLMDADHPYLLATSAEPGAATLVELPGHWALDDWEPYNYLPGITGSGVIS
                     SPAEVLARWTLELEALVGEGGLFMLTNHPFVSGRASRAVALERLIERAQAIDGLWIAT
                     AAEIAAHVATLDLAPVVHRRPAPWA"
BASE COUNT         1142 a         2613 c         2606 g          994 t
ORIGIN      
        1 tgggtacaat tgccatctgc aacgaaccgt ctccgtgcgc cgcccacggg cgacgccaca
       61 tgacccgagg agccgcccga tggccacgac cgccgcatcc ccgaccgacc gtccccatcg
      121 tgccgcgcgg cggtacatct acgcctgggg cgagggcacg gccgagggca acggcggcat
      181 gaaggatctc ctcggtggca agggcgcggg tctcgccgag atgacgatcg cggggctgcc
      241 gacacccccg ggtttcacga tcaccactga agcctgcaat gactacttcg cggccggcga
      301 gcagctcccc gacggtctct gggaggacgt cctcgacgcg atgcgcgagg tcgagcgccg
      361 gtccggcaag ggattcggcg acgccgcgaa cccgctcctc gtcagcgtcc gctccggcgc
      421 caagttctcg atgcctggga tgatggacac cgtcctcaat ctgggcctca acgaggagac
      481 gctccaggga ctggtcgctc tgaccggcaa cgaacggttt ggctgggacg cctatcggcg
      541 gttcatcgag gggtttggcc ggatcgtgat gggagtcgaa ggcgatcgct tcaacgagcg
      601 cctcgacgct cgcaaggaac ggcatggcgc tgacgccaag gacaccgatc tcgacgtcgc
      661 cgatctgaag accctggtcg atgagtacaa gtcgatcgtc cgcgaggata cgggtcgcga
      721 cttcccgacc gacccgaacg aacaactcga cctcgcgatc aaggcggtct tcgcatcgtg
      781 gttcggcaag cgggcaaacg actaccgcaa cagccagaag atcgcccaca acctcgggac
      841 cgcggtcaac atcgtctcca tggtctacgg gaacatgggc gacgattccg ggaccggcgt
      901 cgcgttcacc cggaacacga tcgacggccg gcgcgagctg cagggcgagt tcctgccgaa
      961 cgcccagggc gaagacgtcg ttgccggcat ccggacgccc atcgagatcg ccgagatgaa
     1021 gaaccgctgg cccgaggtct atgccgaatt cgatcggatc ggccaacggc tggagaacca
     1081 ctatcgcgac gtccaggaca tggaattcac gatcgagcgc ggcaagctgt acatgctcca
     1141 gacccgctcg gccaagcgta ccgcggcgtc tgccgtgaaa gtcgccgtcg acatggtcgc
     1201 ggaaggcatg atcacgaagg aagaggccgt cggccggatc gagaccgccc aggtcgacca
     1261 gctcctccga gcccagttcg acccgaaggc gcgcacgagc gcccagcgca tcgccaaggg
     1321 cctcaacgcc tcgcccgggg ccgcggtcgg caaagcggtc ttcgatgccg acgtcgccgt
     1381 cgagtgggtc ggtcgcggcg agaaggtcgt cctcgtccgg gtggagacct cgccagacga
     1441 cttccacggt atggccgtcg cccaggggat cctgaccgcc cgcggtggcg ccacctcgca
     1501 cgccgcggtc gtcgcccgcc agatcggcaa gccctgcgtc gcgggctgcg ccgagctgct
     1561 cgtcgactac ggctcgaaga ccgcacgctc ctccgacagc ggcctgagct tcggcgaggg
     1621 cgactggatc agtgtcgacg gctcgaccgg cgaggtcttc gtcggcgcca tcccgaccgt
     1681 cgaggcccgg ttcgaggacc agcccgagct gcaaacgatc ctcggctggg ccgacggctt
     1741 cagccggatg gcggtctgga ccaacgccga caagcctgag gaagccgccc aggcccggag
     1801 ctacggcgcc cgcggcatcg gcctctgccg gaccgagcac atgttccgcg agggcgagcg
     1861 ccttgacatc gtccgcggcg cgatcctggt cgccggggtc gcgacccggg ccaaggcccg
     1921 tcgcgacgcg aaggcggccg ccgaggcgaa ggcggccaag ggcgatgaca tcagcaccga
     1981 tgagaagacc gcccgagccg tggaactgac tgccgacgaa caggaggcga ttgcgacctt
     2041 cgacgcggcg atggccaagc tcgaggtcct ccagcagggc gacttcgaag gcatcttcaa
     2101 ggcgatggac ggcctcccgg ttgtcgtccg cctgatcgac ccgccgctcc acgagttcct
     2161 gccgaacctc gaggagcagc tcgtcaaggt gacccgagcg gagatggacg gcggcgcgtc
     2221 ggatgaggac aaggagctcc tcgcgacgat ccgctcgctc cacgagcaga acccgatgct
     2281 cgggctgcgc ggctgccgcc tcgggctgat gatcccggac ttcgtgaaga tccagacgcg
     2341 ggcgatcctc aatgccgaga tcgccgtcaa gaagggcggc ggccacccga tcgccaagat
     2401 catgatcccg ctcgtcggcc acgtgaacga gctggccttc acccgggcgc tgctcgagaa
     2461 ggaagccagc gcggtccgcg aggcggcagg cggggtcgag gtcgactaca agttcgggac
     2521 gatgatcgag gtcccgcgcg gggccctcac cgccgacgag atcgcgacgg aggccgactt
     2581 cttcagcttc ggcacgaacg acctcaccca gatgacgttc ggcttcagcc gcgacgacgc
     2641 cgagggtggc tttctcctga agtacgtcga ggatgggatc ctgccggtca acccgttcca
     2701 gacgctcgac gacgcggtcg cggggctgat gaagaccgcg gtcgacaagg gccgctcgac
     2761 gaagcccggc ctcgagctgg ggatctgcgg cgagcacggc ggcgacccgg attcgatcca
     2821 caagtgcgag aagatcgggc tggactacgt gtcctgctcg ccgttccgtg tcccggtcgc
     2881 ccgcctggct gcggcccagg cggcgatggc cggtgcagcc gagcgggaca agtagtcgaa
     2941 cggtctccgg tgcgccgggc ggtcgcgacc ggatcgtggc ggctgaccat cgtcggactc
     3001 ctggcgatgg cgctccgctg cgcggcggtc gtcgcggcgt cgctcaacgc ctgagccctc
     3061 agtacgagcc ggcgtccgcg agcacctcgt cctcgggcag gtacgggacg tagtccggcc
     3121 accacatcgc ccggtcgacg gccgcgtcga cgtcctcgtc gctcgtctga tcgcagcccg
     3181 cgatcccgag ccggaccacg gtccgggcga ccgccgtggc gatggcccgc gtgaccgagc
     3241 gcagctccga gaccgatggg tagatcaccc cggccgcgag ccgctcgttc gacacgcagg
     3301 tcgcgagcgt ccgggcggca atgaggaaca gctcgtcggg catcctcgcc gcctcggcga
     3361 cgatcgcccc gagccccagt cccgggaaca cgaagacgtt gttcgcctgg ccgatctcga
     3421 ctcgccggcc gtcgtactcg acggcggcga agggcgaccc ggtcgcgacg atcgcccggc
     3481 cacccgacca gcggaagatg tcggccggaa tcgcctcggt ggccgtcgtc gggttggaca
     3541 gcggcatgac gatcggccgc tcgaccccgg cggcgacgtc ggagatcagg gcctcatcga
     3601 acgtacccgg cgttccggtc gccccgagca ggatcgtcgg ccggagggcg cggaccgtgg
     3661 cgtcgagcgt cggcgtcgtc gagaagtcgc cgaacccctc cgcggtcagt cgggcgaagg
     3721 gtcgggccac ctcgcgcttg tcgtcgtcga gatccgcccg gccgtcgtgg acgaggccgt
     3781 gggtgtcgac catcgcgatc gccacggtca cctcgtcggg tgtcgccccg tcctcgatca
     3841 tcgccaggcg cagcagccgg gcgatcccga tccccgccgc accggcgcca gcgatcacga
     3901 cccgctggtc gacgagtcgg ccgcccagcg agcggacggc ggcgaggatc ccgccgagga
     3961 cgaccgcggc cgtgccctgg atgtcgtcgt tgaacgagca gaaccggtcg cgataacggt
     4021 cgaggatccg gagcgcgttg tgctgcttga agtcctccca ctggatgacg cagtcgggcc
     4081 atgtggtctc gaccccggcg acgaacgcct ccacgagcgc gtcgtactcg gcgccacgga
     4141 ggcgcggccc ccgatggccg agatacagcg ggtcggcgag gaggagcggg ttgtccgtcc
     4201 cgacgtcgag cgagaccggc agggtcagcg ccggatggat cccggacgcg gcggtgtaga
     4261 gcgccagctt gccgatcggg atggccatcc cgcctgcgcc ctggtcacca agcccgagga
     4321 tccgctcgtt gtcggtcacg acgatcaacc ggacgtcgtc gtaggggctg ttccggagca
     4381 ggtccgggat ccggtcgatg tccgccgggg tgatccacag accccgggtg cggcggatga
     4441 tgtggctgaa ttcctggcag gcgcgtccga ccgtgggggt gtagacgatc gggaggaact
     4501 cctcggcgtg ctcggtgagg agccggtagt acagcgtcgc gttgcggtcc tggagcgcgg
     4561 ccagcccgat gtagcgttcg agcgggtccg ccttgcgccg caggtgctca agctcgagct
     4621 cgacctgctc ctcgatcgtc aggacccgat ccggcaggag gccatcgagg ccgaacgcca
     4681 cgcgttccgc gggcgggaag gcgaggtcct tggtcaggag ccgcgcgccg agcagggccg
     4741 acgagcggag gccgatccag gccgggcgcc ccgccgcgat ctcttcgaag ggagcgggga
     4801 ggggtcgcac ggttcgtcga gtctaggccc gcgcgcgccg ctcgtcgcgt gaccgatggc
     4861 ccgttctgac cgggtcgctc gggacgggct cgtccacctg atcgggtttg ccaaccggct
     4921 cgtcctcacg ggtcgctgag cctggcgctc ctgaaccacc gccgcggatc cagtctggcc
     4981 gcgccgtcgt cgcgcgtccg accgacaccg tgatgtccag gaagcactgt ttctggcggc
     5041 aggccagcga acactgcacg attcgggcga ttccgtcccg gcgcgatgcc tcacccacat
     5101 cgaacggccg cgaagacgct ccaccgagcc gatccaccag tcttcacgcc ggaaacgatg
     5161 catgtcaggc atcgccgcgc gggcgagccg ccttcgctcg cgaccgtcag aacgcgatga
     5221 tcgagcggac gccgtcgccg cggcgcatcg ccgcgaacgc cgcctcgatc ccgtcgaggc
     5281 cgatccgggc ggtcaccagc cgctccgcgg gaagccggcc ggcgagatgg agcgccgcgt
     5341 aacgcgggaa gtcgaccgcc ggatcggccg agccgtagtt cgagccgagg atccgccgcc
     5401 cgccgtcgac gaacgggaac ggctcgaacg acgcccgcac tccgaacggc gtcatcccga
     5461 cgaggacggc cgtgccgccg gtcggcaggc aggcgatcgc ggtctcgatc gtggccggca
     5521 gcccgatcgc ctcgaagcag gtgtccggcc cgccgtcggt cgcagcgcgg atcgcggcca
     5581 gggtctcggc ggcgtcggag ccggccagga gaccgtccgt cgcgcccacc gcgcgggcca
     5641 gctccagttt ggctgcaacc cgatcgaccg cgacgatccg gccggcgccc gcgagcgccg
     5701 cgcccatgac gcacgatagc ccgaccccgc cgagcccgat cacgacgacc gaagagccgg
     5761 gctggacctc ggcggtcttc gtcgcggcgc cgacgcccgt ggtgacgcag cagccgatga
     5821 gcgccgcgac ctcgggcggc gtgccgtcag gcatcgggat cgcggccgcc gcgggcacgt
     5881 tctggcggtc ggcgaacgtg ccgatcgcgc agtacgtgag gacgtcgccg ccgccggccg
     5941 gccggtggag cgctgcggca ccgtcgggca tccggtgggt gtaggacggg ctctcggagc
     6001 agctccagac ccggccggcc cggcaggcgc ggcagcgacc gcacgggatc agccaggcga
     6061 gggcgacgag gacgccgggt ttgagcccgg gaaacgccgc cgccacgccc tcgccgagcg
     6121 cctcgacgac cgccgagccc tcgtggccga ggacgatcgg cccctcgcgc tgccactcgc
     6181 cgtcgcggac gtggaggtcc gagtggcaga cccccgcggc cgccatcctg acgcggacct
     6241 cgcccgggcc gggcgggtcg agcaggagcg tctcgatccg gacggcgacg cccggtcggt
     6301 cgacgacggc ggcgcggatc tcgacgggct cggccatgga cgcaggatcg cacgtcgcag
     6361 ggagcctgtg gctgcgtaga ctctcggcgt gccgcacgac gtcccagagg attggcgctc
     6421 gccgctgccg ccgggcttcc gctggccgga cggcaagcgc gcggcggcgt gcctgacgtt
     6481 cgatgtcgac gcggagagcc cgatcctgtt cgagcacccg gaaagcgcgg cctggctcga
     6541 cgtcatgacg caccaggcgt atggggcgcg gaccgggatc gctcggctgt tgcggctcct
     6601 cgaccggcgc cgtgtccggg cgacgttctt catccccggc tacagcgccg agcgctggcc
     6661 ggagatctgc cgtgcgatcc gcgatgcggg ccacgagatc gcccaccacg gctatctcca
     6721 cgagggtgcg cacggtgcga ccccggccga cgaggagagc cgcctgctgc gcggcctggc
     6781 ggcgctcgac gaggtcctcg agatccggcc gacgggttac cgcggaccga actgggagat
     6841 gacctacgag acgccggcgc tcctcgcccg ccacggcttc acctacgact ccgggctgat
     6901 ggacgccgac cacccgtacc tgctcgccac ctctgccgag ccgggcgccg cgacgctcgt
     6961 cgagctgccg ggccactggg ccctcgacga ttgggagccg tacaactacc tgcccgggat
     7021 caccggttcc ggcgtgatct cgagcccggc cgaggtcctc gcccgctgga cgctcgagct
     7081 cgaggcgctc gtcggcgagg gcggcctgtt catgctgacc aatcacccgt tcgtgtccgg
     7141 ccgcgcctcc cgcgccgtcg ccctggagcg cctgatcgag cgggcccagg cgatcgacgg
     7201 cctgtggatc gcgaccgccg ccgagatcgc cgcccacgtc gcgacgctcg acctcgcgcc
     7261 ggtcgtccac cgccggccgg cgccctgggc ttgagcccgg ggaatcgcgc gggtgccgcc
     7321 gaccggagcc tgacgattcc cgttgcgcgg gccgg
//
LOCUS       DATAIW010000068         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_2702, whole genome shotgun sequence.
ACCESSION   DATAIW010000068 DATAIW010000000
VERSION     DATAIW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1281)
                     /gene="metK"
                     /locus_tag="VHM48_02210"
     CDS             complement(58..1281)
                     /gene="metK"
                     /locus_tag="VHM48_02210"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2754245.1"
                     /translation="MTSIVDASHYLFTSESVTEGHPDKMCDQISDAILDAIIGDDPYA
                     RVACETATTTGLVLVLGEITTTTYIDFQTVVRDTVRDIGYTKAEYGFDYETCGTLVSV
                     KGQSPDIAQGVNSALEVREDQSANELGAGDQGMMFGFACRETPELMPLPIALAHRMAR
                     RLAEVRKTGQLPYLRPDGKTQVTVEYERGIPKRVRTVVLAAQHDPDVSQERLHSEIVD
                     TVILPSIPADLRETDPVMHVNPTGRFVTGGPMGDAGVTGRKIIVDSYGGMARHGGGAF
                     SGKDPTKVDRSAAYAARWVAKNVVAAGLADRFEIELAYGIGIARPISFSVESFGTGKL
                     DDEDIQRLVERHFDLRPAAIIAALDLRRPIYRQTASYGHFGRLDLDLPWERTDMAALL
                     ASEAGLALPEAVPAG"
     gene            1354..2652
                     /gene="kynU"
                     /locus_tag="VHM48_02215"
     CDS             1354..2652
                     /gene="kynU"
                     /locus_tag="VHM48_02215"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX2754246.1"
                     /translation="MDPAIDPGAHVEDGPRPDPAVLDAAGLDAAGLDAADPLASFRRR
                     FVMPDPDLVYLDGNSLGRPPLRAIERVSRVVSEEWAGELIRGWDHWLAAPRRVGDLLG
                     TGLLGARPDEVAICDSTTVDFFRLASAALDARPGRQIIVTDRANFPTDRYVLEGLARD
                     RDREIAWLDPDPIDGPSADEVAAVLAAHPGDIALVTLSHVNYRSAAIADLPAITDLAH
                     GAGALVLWDLSHSAGSVPVGLAEHGVDLAVGCTYKYLNGGPGAPAWLYVRTDLQEELR
                     NPIQGWFGQTDQFEMGQGFRPMAGIGGWLTGTPGVIGLAAAEEGIRVSVEAGIDAIRA
                     KGIALTEYAIALHDEWLAPLGFTLGSPRAAARRGSHVSVRRADARQLTRGLIEAGVLP
                     DFRAPDSIRLGLSPLTTSFTDVARGIAVLARLAGSRPASA"
     gene            2671..2931
                     /locus_tag="VHM48_02220"
     CDS             2671..2931
                     /locus_tag="VHM48_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754247.1"
                     /translation="MSGPRADDSFEIPIERLAEGRGQRRRAAIAVASLAIVVGGAIGL
                     ARLAESRATQIILVDLADRTRITAITLPMNGPASIIAVELRP"
BASE COUNT          422 a         1059 c         1044 g          451 t
ORIGIN      
        1 acgattcgat cgcggacagg aagacggcga gcgccggggt gggcgcggcg ctgccgctca
       61 tccggcgggg acggcctccg gcagagcgag acccgcctcc gacgccagga gggcggccat
      121 gtcggtccgc tcccacggca ggtcgagatc cagccggccg aagtgaccgt aggaggccgt
      181 ctggcgatag atcggccggc ggagatcgag ggccgcgatg atcgccgccg gacggagatc
      241 gaagtggcgc tcgacgaggc gttggatgtc ctcgtcgtcg agcttgccgg tcccgaagga
      301 ctcgaccgag aacgagatcg gccgggcgat cccgatgccg tacgcgagct cgatctcgaa
      361 ccggtccgcg aggccggcgg cgacgacgtt cttggcgacc caacgcgcgg cgtatgccgc
      421 cgaccgatcg accttcgtcg gatccttgcc cgagaacgct ccaccgccat ggcgggccat
      481 cccgccgtag ctgtcgacga tgatcttgcg gccggtcacg ccggcgtcgc ccatcgggcc
      541 gcccgtgacg aagcggccgg tcgggttgac gtgcatcacc ggatccgtct cgcggaggtc
      601 ggcgggaatg ctcggcagga tcaccgtgtc gacgatctcg ctgtggagcc gctcctggct
      661 gacgtccgga tcgtgctggg ccgcgaggac gaccgtgcga acccgcttcg ggatgccccg
      721 ctcgtattcg accgtgacct gggtcttgcc atccgggcgg aggtacggca gctggccggt
      781 cttgcgaacc tccgccaggc gacgggccat gcgatgggcc agcgcgatcg gaagcggcat
      841 gagctccggt gtctcacggc aggcgaaccc gaacatcatc ccctggtcgc cggcgccgag
      901 ctcgttggcg gactgatcct cgcggacctc gagcgccgaa ttcacgccct gggcgatgtc
      961 cggcgactgg cccttgaccg agacgagcgt gccgcaggtc tcgtagtcga agccatactc
     1021 cgccttcgtg tagccgatgt cgcgaaccgt gtcgcggacg acggtctgga agtcgatgta
     1081 ggtcgtcgtc gtgatctcgc cgaggacgag caccaggccc gtcgtggtcg cggtctcaca
     1141 ggcgacccgg gcgtaggggt cgtcgccgat gatcgcatca aggatcgcat ccgagatctg
     1201 gtcgcacatc ttgtcggggt gtccctcggt gaccgattcc gaggtgaaca ggtagtgcga
     1261 tgcgtcgacg atgctggtca tggggccctc tgcttggatt gcccgccagc gtatcaccgg
     1321 atcgggatcc ggctcccggg ctagactccc ggagtggatc cagcgatcga cccgggtgcc
     1381 catgtcgagg acggtccacg acccgacccc gcggtcctgg acgcggccgg actcgacgcc
     1441 gccggcctcg acgcggccga tccgctcgcc tcgttccggc ggcggttcgt catgcccgat
     1501 cccgatctcg tctacctcga cggcaactcg ctcgggcgtc caccgctccg ggccatcgaa
     1561 cgggtctcgc gggtcgtcag cgaggaatgg gcgggcgagc tgatccgcgg ctgggaccac
     1621 tggcttgcgg caccgcgtcg ggtcggcgac ctcctcggga cggggctcct cggggcacgg
     1681 ccggacgaag tcgcgatctg cgactcgacc acggtcgact tcttccggct cgccagcgcg
     1741 gccctcgacg cacggcccgg ccggcagatc atcgtgacgg atcgggcgaa cttcccgacc
     1801 gaccgctacg tcctcgaagg cctcgcccgc gaccgcgacc gcgagatcgc ctggctcgac
     1861 ccggacccga tcgacggccc atcagcagat gaggtcgccg ccgtcctcgc tgcccacccg
     1921 ggcgacattg ccctcgtgac gctgagccac gtcaactacc gctccgcggc catcgccgac
     1981 ctgccggcga tcaccgacct cgcccatggc gccggcgcgc tggtcctgtg ggacctctcg
     2041 cactccgcgg gttcggtacc ggtcgggctc gccgagcacg gcgtcgatct ggcggtcggc
     2101 tgcacgtaca agtacctcaa cggcggtccg ggcgcacccg cctggctgta cgtccggacg
     2161 gacctccagg aagagctccg taacccgatc cagggctggt tcggccagac tgaccagttc
     2221 gagatgggcc agggcttccg gccgatggcc gggatcggcg gctggctcac gggaacgccc
     2281 ggcgtcatcg gcctggcggc agccgaggag ggaatccgcg tctcggtcga ggccgggatc
     2341 gacgcgatcc gggccaaggg catcgccctg accgagtacg cgatcgccct ccacgacgaa
     2401 tggctggcgc cgctcgggtt caccctcggc agcccgcgag ctgccgcccg gcgcggctcc
     2461 cacgtgtccg ttcgccgggc agacgcgcgc caactgacgc gaggactgat cgaagccggc
     2521 gtcctgcccg atttccgcgc gccggattcg atccggctgg gcctctcgcc gctgaccacg
     2581 agcttcaccg atgtcgctcg cgggatcgcc gtcctcgccc ggctcgccgg cagccggccc
     2641 gcgagcgcct gagcccgggc gcccatccgg atgtccgggc cgcgcgccga cgattcgttc
     2701 gagatcccga tcgagcgcct ggccgaaggc cgcggccagc gtcgccgggc ggcgatcgcg
     2761 gtcgcgtccc tcgcgatcgt ggtgggcggc gcgatcggcc tggctcggct ggctgaatcc
     2821 agggcgaccc agatcatcct cgttgacctg gctgatcgga cccggatcac ggcgatcacg
     2881 cttccgatga acggaccggc atcgatcatc gcggtggagc tgcggccatg atccgccgcg
     2941 cgacgaatca ggccggcgtc gcgcgtcgcg ggccgg
//
LOCUS       DATAIW010000069         6939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_151922, whole genome shotgun sequence.
ACCESSION   DATAIW010000069 DATAIW010000000
VERSION     DATAIW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151922"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VHM48_02225"
     CDS             complement(<1..1571)
                     /locus_tag="VHM48_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754248.1"
                     /translation="MIGLLRTYLRPYRGRLLLVMGLLLVQAIGNLYLPDLNADIINNG
                     VVLGDNDYIIRTGALMLLVTGLLAITAVIGVYWGARVAMGFGRDVRSAIFTKVETFSQ
                     VEVNHFGPASLITRNTNDVQQVQLVVFMGLTIMISAPILIVGGVIMALRTDVPLSGLL
                     LIVLPLMAAVIALVMSRAVPLFRAVQLKIDRINQVMRETLAGVRVIRAFVRTSHEEQR
                     FDTASRDLFDTSLQVTRLFAITIPTMTAIFNLSTVAVIWFGAMRVNDGGLSIGNLTAF
                     MQYLAQILFAVLTAVFMFILIPRAAVSSGRIREVLDTEPLIREPERPITPSDPARRGE
                     VIFDAVEFRYPGAEEPVLREIAFRARPGETTAIVGSTGSGKSTLINLIPRFYDASMGS
                     VHVDGVDVREMGLEDLWQRIGIIPQKAFLFAGTVASNLRFGDENATDHELWHALEIAQ
                     AKDFVEEMDGGLEASITQGGTNLSGGQRQRLAIARALVKKAGIYIFDDSFSALDFATD
                     ARLRAALDREISWAT"
     gene            complement(1568..2062)
                     /locus_tag="VHM48_02230"
     CDS             complement(1568..2062)
                     /locus_tag="VHM48_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2754249.1"
                     /translation="MDPGSSRPVDVGRDALIAELVDEMTAWSPRERMSAFRSWLKGSL
                     SLIHLNVLTVLEADGPLPMSHLAEELDVSVASTTGIVGRMEERGLVERSHGEADRRVV
                     LVSPTAAGIAVFRDMTEHRREQLTKLFGRLTDDELAALLTGLRAMRTARLAIHAEMGS
                     GDET"
     gene            complement(2121..2984)
                     /locus_tag="VHM48_02235"
     CDS             complement(2121..2984)
                     /locus_tag="VHM48_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX2754250.1"
                     /translation="MELPLFPLNTVLCPGIALPLHVFEDRYRAMVRHCLETTSPFGVV
                     LIRQGREVGSGVISFTGIGTIAEIRDAGSYDDGRYDLLVVGTRRFEIRHVLSGKQPYL
                     VAEVDLLDEAIGDEQVAQQLAMRATRRFVTYLELLQPRSGETADEIDVRVEIETEDDE
                     PGDEPGDEPEAGLDDLEAEPDDDPVAGSVAAAGDLDAAGAAVPPDGSGSDAPRRRRVM
                     IPDDPTVLSHLLAGILQVESPRRQALLEAETTEDRLRGLLTLIDRETFLLQRRLRFFQ
                     PESRVTGPRRS"
     gene            3146..3769
                     /locus_tag="VHM48_02240"
     CDS             3146..3769
                     /locus_tag="VHM48_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754251.1"
                     /translation="MAYRGDEGSMDWYRSKQAIVARLDGVVGRLAEAGVSPDAVTVSA
                     IPLAVVGGLCLLASPVVPLALVVVPVAAALRLATNLVDGALARATARTHPRGELLNEL
                     CDRIADLALLGPVAFLPGAQRETVLLGLTGAVFASFVGVASRAAGGARLYRGVLSKPG
                     RMALLAVSAIAVLIVGPGAWGPFGPLLLLGTTLTVIERTVVAIRGLP"
     gene            3766..4710
                     /locus_tag="VHM48_02245"
     CDS             3766..4710
                     /locus_tag="VHM48_02245"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2754252.1"
                     /translation="MNPFPYTTIVLGGGVALGLLLASGWALLRRGRDAQPATKGGTAG
                     RTVLARAASYAGLAAVLGVASWLGTPGIAILAVALGALGLSEWSNLADLPVHHRLALQ
                     AANVAIIALVAALGTRAAEWIVGGAILAGMVWPVLRPDPSRAMRDLGFAALGTILLPG
                     MLAHAVALVVERGALGAATFVALAVAVAFSDIGAFLVGRRFGRTPLSPLLSPAKTRAG
                     IVGNLLGAAVGVAAFGPVLVTGFGIGFAVLLVPIIALGAVWGDLLESAAKREAGQKDA
                     GRWLPGFGGILDRIDSLLITLPLVFWSLRLVDLIGTAR"
     gene            4707..5339
                     /locus_tag="VHM48_02250"
     CDS             4707..5339
                     /locus_tag="VHM48_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2754253.1"
                     /translation="MSWQFSTPAVIARETTARLVGPLVSALVGGPRVVGRERLDDLDG
                     PFVICPNHSSHLDVSTLRLALGRRHRRRLAAAAAEDYFFTGHRSRAFLAAWLGGFAFR
                     RRGANPGSVAEIDRLLDAGWSVLLFPEGTRSRDGAIAPFKPGIGLIATRTGRPVVPVR
                     IDGLHDVLAPGRRLPHRGRVEVRFGEALRAQPGEGPREFTARLEAVVRAL"
     gene            5362..5976
                     /locus_tag="VHM48_02255"
     CDS             5362..5976
                     /locus_tag="VHM48_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754254.1"
                     /translation="MPDTAWFAVPIEPFATLAAEPLILIVLAAWGVGEALVLPVVPDV
                     LLGLLVLAAPWQVGPLLAAAIVGGVAGAVPGWWLLRHRRSLAERVLALQPGLGQRGLD
                     QAEDRIRRRGPLLAFAQVGPGLPLKAYVHAIDVVAPATTPATVAGLALVNRLARLGPV
                     ALVFAALHPFAVGWPPAVLAVTWIGGWTAFYVAYWVARDPRRRG"
     gene            complement(6077..6457)
                     /gene="erpA"
                     /locus_tag="VHM48_02260"
     CDS             complement(6077..6457)
                     /gene="erpA"
                     /locus_tag="VHM48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX2754255.1"
                     /translation="MLDIQPTVVSLSDAAASKLRELTAEETNPDVGLRVYVYSGGCSG
                     FRYGMMLEDAPTAEDKILEANGVKVYVDGKSIDLLKGSEIDYVDTLMGAGFTVNNPNA
                     VAACGCGSSFRTADDTGSPKGCSH"
BASE COUNT          915 a         2411 c         2503 g         1110 t
ORIGIN      
        1 atcgtggccc acgagatctc ccggtcgagc gcggcgcgga gccgggcgtc ggtcgcgaag
       61 tcgagggcgg agaagctgtc gtcgaagatg tagatcccgg ccttcttgac gagggcgcgg
      121 gcgatcgcca ggcgctgccg ctggccaccc gacaggttgg tcccgccctg ggtgatggat
      181 gcctcgaggc cgccgtccat ctcctcgacg aagtccttcg cctgggcgat ctcgagggcg
      241 tgccagagtt cgtggtccgt ggcgttctcg tcgccgaagc ggaggttgct cgcgaccgtc
      301 ccggcgaaca ggaacgcctt ctgcggaatg atcccgatcc gctgccagag gtcctcgagc
      361 cccatctcgc ggacgtcgac gccgtcgacg tggaccgatc ccatggaggc gtcatagaag
      421 cgggggatca ggttgatcag ggtcgacttg ccactgccgg tgctgccgac gatcgccgtc
      481 gtctcgcccg gccgggcgcg gaacgcgatc tcgcgaagga ccggttcctc ggcccccgga
      541 tagcggaact cgacggcgtc gaagatgacc tcgccgcgcc gggccgggtc gctcggcgtg
      601 atcggccgct ccggctcgcg gatcagcggc tcggtgtcga ggacctcgcg gatccggccg
      661 ctcgagaccg cggcgcgggg gatgagaatg aacatgaaga ccgccgtcag gacggcgaag
      721 aggatctggg cgaggtactg catgaacgcg gtgaggttgc cgatcgacag ccccccgtca
      781 ttgacccgca tcgccccgaa ccagatgacg gccacggtcg agaggttgaa gatcgcggtc
      841 atcgtcggga tcgtgatcgc gaacagccgg gtgacctgga gggaggtatc gaacaggtcg
      901 cggctggcgg tgtcgaagcg ctgctcctcg tgcgaggtcc ggacgaacgc gcggatgacc
      961 cggacgccgg ccagcgtctc gcgcatgacc tggttgatcc ggtcgatctt gagctggacc
     1021 gcccggaaga ggggcacggc ccgcgacatg accagggcga tcacggcggc catgagcggc
     1081 agaacgatca ggagcaggcc ggacaggggg acgtccgtcc ggagggccat gatcacgccg
     1141 ccgacgatca ggatcggggc cgagatcatg atcgtcaggc ccatgaagac gacgagctgg
     1201 acctgctgga cgtcgttcgt gttgcgggtg atcagcgacg ccgggccgaa gtggttgacc
     1261 tcgacctggg agaacgtctc gaccttcgtg aagatggcgc tgcggacgtc gcgcccgaag
     1321 cccatcgcga cccgcgcgcc ccagtacacc ccgatgacgg cggtgatcgc gaggagcccg
     1381 gtgacgagca gcatgagggc gcccgtccgg atgatgtagt cgttgtcccc caggaccacg
     1441 ccgttgttga tgatgtcggc gttgagatcg ggcaggtaca ggttgccgat cgcctggacc
     1501 aggaggaggc ccatcaccag gagcagtcgg ccgcggtacg ggcggagata ggtccgcaac
     1561 aggccgatca cgtctcgtct ccgcttccca tctcggcgtg gatcgcgagg cgagcggtgc
     1621 gcatcgctcg aaggcccgtg agcagcgcgg cgagctcgtc gtccgtcagt cgcccgaaga
     1681 gcttcgtgag ctgctcgcgg cgatgctcgg tcatgtcgcg gaacacggcg atcccggcgg
     1741 cggtcggact gacgaggacg actcgtcggt cggcttcgcc gtggctccgc tcgacgaggc
     1801 cgcgttcctc catccggccg acgatcccgg tcgtgctagc gaccgagacg tcgagctctt
     1861 cggcgaggtg ggacatcggc agcggtccat cggcctcgag gacggtcaga acgttgaggt
     1921 ggatcaggct gagcgagccc ttgagccagg atcggaacgc gctcatccgt tcgcgcgggc
     1981 tccacgcggt catctcgtcg acgagctcgg cgatcagggc gtctcggccg acgtcgaccg
     2041 gtcgagagct gcccgggtcc accgagttat tcaatatcac atgaataatc tatccggacc
     2101 atagcgtggt gtcaatccgc tcagctccga cgcggaccgg tgacccgcga ttcaggttgg
     2161 aagaaccgga gccgccgctg gagcaggaac gtctcgcggt cgatcaaggt gagcaggccg
     2221 cggaggcggt cctcggtcgt ctcggcttcg agcagcgcct ggcggcgggg cgattcgacc
     2281 tggaggatgc cggccagcag gtgcgagagc acggtcggat cgtccgggat catgacccgt
     2341 cgtcgccgcg gggcgtccga cccgctccca tcgggcggaa cggcggcccc ggccgcgtcg
     2401 aggtcgccgg ccgcggccac ggatccggca accgggtcgt cgtcgggctc ggcctccagg
     2461 tcgtcgagtc cggcttcggg ttcgtcgccg ggttcgtcgc cgggttcgtc gtcctcggtc
     2521 tcgatctcga cccggacgtc gatctcgtcg gcggtctcgc ccgatcgcgg ctggagcagt
     2581 tcgaggtacg tcacgaacct ccgcgtcgcc cgcatcgcga gctgctgagc gacctgctcg
     2641 tcaccgatcg cctcgtcgag caggtcgacc tccgcgacaa ggtagggctg cttgccgctc
     2701 aggacgtggc ggatctcgaa gcgccgggtc ccgacgacaa gaaggtcgta gcggccgtcg
     2761 tcgtatgacc cggcgtcccg gatctcggcg atcgtgccga tcccggtgaa cgagatcacg
     2821 cccgagccaa cctcccggcc ctggcgaatc agcaccacgc cgaacggcga cgtcgtctcg
     2881 aggcagtggc ggaccatcgc ccgatagcga tcctcgaaga cgtggagcgg cagcgcgatg
     2941 cccggacaca ggaccgtgtt cagcgggaag aggggcagtt ccatccggcg gtcatccatg
     3001 cgacgtcggg tggctcgggc gatcgaggcg tgtcctgcgc agtctagggt ttcggctggc
     3061 cggcagacac gacgcgcccc gctgcctccg tccgcccgca acagccgcgc gcccggcgcg
     3121 tccacgcagt atcacgcccg atggcatggc gtaccgtggc gacgagggat cgatggactg
     3181 gtaccgctcg aaacaggcga tcgtggcccg gctcgatggc gtcgtcggac gccttgccga
     3241 agccggtgtc tcacccgacg cggtcaccgt ttcggcgatc ccgctggcag tcgttggtgg
     3301 cttgtgcctc ctggcctcgc ccgttgtgcc cctcgccctc gtcgtcgtcc cggtcgcggc
     3361 cgctctgcgg ctggcgacga acctcgtcga cggagccctg gcccgagcga ccgctcggac
     3421 ccacccccgg ggcgagctgc tcaacgagct ctgcgatcgg atcgccgacc tggccctcct
     3481 cgggcccgtc gccttcctcc ccggtgccca gcgggagacg gtcctgctcg gtctgaccgg
     3541 cgcggtgttc gcctcgttcg tcggggtcgc atcgcgggcc gccggcgggg cgcggctgta
     3601 ccgcggagtc ctctccaagc cggggcggat ggcgctcctc gccgtgtccg ccatcgcggt
     3661 cctgatcgtc gggcccgggg cctggggccc gttcgggccg ctcctgctcc tggggacgac
     3721 attgaccgtg atcgagcgaa ccgtggtcgc gatccggggc ctgccatgaa cccattcccg
     3781 tacaccacga tcgtgctcgg cggcggcgtt gcgctcggtc tgctgctcgc gtccggctgg
     3841 gcactccttc gtcgggggcg cgacgcgcag cccgcgacca aaggcgggac cgcgggccga
     3901 accgtcctcg cccgggcggc cagctacgcc gggctggcgg ccgtgctcgg cgtggcgtcg
     3961 tggctcggaa cgccggggat cgcgatcctg gccgtggcgc tcggtgcgct cgggctcagc
     4021 gagtggagca acctcgccga tcttccggtc catcaccggc tggccctgca ggcagccaac
     4081 gtcgcaatca tcgcgctcgt cgcggcgctc ggcacgcggg cggccgagtg gatcgtcggc
     4141 ggagcgatcc tcgccggcat ggtctggccg gtcctgcgac cggacccgag ccgggcgatg
     4201 cgcgacctcg gcttcgccgc gctcgggacg atcctcctgc ccggcatgct ggcccatgcg
     4261 gtcgcactgg tcgtcgagcg gggcgcgctc ggcgccgcga cgttcgtcgc cctcgcggtc
     4321 gccgtcgcct tctcggacat cggcgcgttc ctggtcggac ggcggttcgg ccggacgccg
     4381 ctctcgcccc tgctgtcgcc ggcgaagacc cgggccggta tcgtgggcaa cctgctcggc
     4441 gcggctgtcg gcgtcgccgc gttcgggccg gtcctcgtca cgggcttcgg gatcgggttc
     4501 gccgtgctcc tcgtgccgat catcgccctc ggtgccgtgt ggggcgacct gctcgagtcg
     4561 gcggccaagc gggaggcggg ccagaaggac gccggacgct ggctgccggg cttcggcggg
     4621 atcctcgatc ggattgactc tctgctgatc acgcttccgc tcgtgttctg gtccctgcgc
     4681 ctggtcgacc tgatcgggac cgcgcgatga gctggcagtt ctcgacgccg gcggtcatcg
     4741 cacgggagac caccgcccgg ctcgtcggcc cgctggtgtc cgccctcgtc ggcgggccgc
     4801 gggtcgtcgg ccgggagcgt ctcgacgacc tcgatgggcc gttcgtcatc tgccccaatc
     4861 acagcagcca cctcgacgtc tcgaccctcc gcctggccct cggccggcgc caccggcgac
     4921 ggctggctgc ggcggccgcc gaggactact tcttcaccgg ccaccgcagc cgggcgttcc
     4981 tcgcggcgtg gctgggcggc ttcgccttcc gccggcgcgg cgccaacccc gggtccgtcg
     5041 ccgagatcga tcgactgctc gacgccggtt ggagcgtcct gctgttcccg gaaggaaccc
     5101 gtagcaggga cggcgcgatc gccccgttca agcccgggat cggcctgatc gcgacgcgca
     5161 ccggacggcc ggtggtcccg gtccggatcg acggtctcca cgacgtcctg gcgcccggcc
     5221 gacggctccc ccaccgcggc cgggtcgagg tccggttcgg tgaggcgctg cgcgcccagc
     5281 ccggcgaggg cccgcgcgaa ttcaccgccc ggctcgaagc cgtggtccgg gcgctgtgag
     5341 gtgcccgcgg tcggactcct gatgcccgac acggcctggt tcgcggtgcc gatcgagccc
     5401 ttcgccaccc tcgccgccga gccgctcatc ctgatcgtcc tcgccgcctg gggcgtcggc
     5461 gaggcgctgg tgttgcccgt cgtgccggac gtcctgctcg ggctcctcgt gcttgcggct
     5521 ccgtggcagg tcggcccgtt gctcgccgcc gcgatcgtgg gcggggtggc cggcgccgtc
     5581 cccggctggt ggctcctgcg gcatcgccga tcgctcgccg agcgggtgct ggcactgcag
     5641 ccgggcctcg gccaacgcgg cctcgatcag gccgaggacc ggatccggcg gcggggcccg
     5701 ctcctggcgt ttgcccaggt cggcccgggt ctgcccctca aggcctacgt ccatgccatc
     5761 gacgtcgtcg ctccggccac gacgccggcg acggttgccg ggcttgccct cgtcaatcgg
     5821 ctcgctcggc tcgggccggt cgcgctcgtg ttcgcggccc tccacccgtt cgcggtcggc
     5881 tggccgccgg ccgtgctggc ggtcacctgg atcggcgggt ggacggcctt ctacgtcgcc
     5941 tactgggtcg cccgcgatcc gcgtcgtcgc ggctgagagg tcggtcgatc gccgccggac
     6001 gggtatcgaa acagccgccg gacgggccgg cggctgctgg acgttcaggt agaggcgacg
     6061 gaggccgtcg cgccggtcag tgggagcagc ccttgggcga gccggtgtcg tcggcggtcc
     6121 ggaagctcga gccgcagcca caggccgcga ccgcgttcgg gttgttgacc gtgaagccgg
     6181 cgcccatcag cgtgtcgacg tagtcgatct cggagccctt gaggaggtcg atcgacttgc
     6241 cgtcgacgta gaccttgacg ccgttcgcct cgaggatctt gtcctcagcg gtgggcgcgt
     6301 cctcgagcat catcccgtag cggaagccgg agcagccgcc ggagtagacg tagacgcgca
     6361 ggccgacatc ggggttcgtt tcctcggcgg tcagctcgcg gagcttcgac gcggcggcgt
     6421 ccgacaggct gacgacggtc ggctgaatgt ccaacatgcg gtgaggcctc ctccggaggg
     6481 ctgcggggtg ctcggaagtc tcgctgttct cgaggacgtc cgtcgatggc ccgatcgtac
     6541 gcggcgcaaa ggattccggc aagagtggcg ccgaaacccc gaactgagtc gctccatgtt
     6601 gaatgccgaa gcatcctgac ccgtcgcaag ggggcaggtg cccgcgaacc gttccggccg
     6661 cccatctttc ccatacgcca gccgacgtgg catcaagcgg gccacagggt ccgatccgtg
     6721 cgaatcgtgg cgtgagttag ccgtggaaga ctgacaagtg ggccgaacgg tcggcttccg
     6781 accccgatcg accatcgccg gggcgctaac gcccgcgggg ctagcgtatg gggaaatccg
     6841 gttccggcgg cacgcccggg cgccacgcgt ccggctggac ggtgcggccc cggcgccgcg
     6901 cgtcagccgt cgatccgctc gatgaccgtc gcgatcccc
//
LOCUS       DATAIW010000070         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_32936, whole genome shotgun sequence.
ACCESSION   DATAIW010000070 DATAIW010000000
VERSION     DATAIW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32936"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VHM48_02265"
     CDS             complement(<1..335)
                     /locus_tag="VHM48_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX2754256.1"
                     /translation="MATTRRATATWSGDLLSGNGTVTAATTGIFRDQPTTWASRTGEP
                     EGVTSPEELLAAAHASCFSMACSNELAKAGTPPTALAVQVDVSADKLEAGWTVTAAAI
                     TVRATVPGAT"
     gene            591..1433
                     /locus_tag="VHM48_02270"
     CDS             591..1433
                     /locus_tag="VHM48_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2754257.1"
                     /translation="MVLTLPSLTRRRLASLALVAVLGAVIAAPGAATATVTTVDDATL
                     TAAETAMVSALNADRTDRGLVAVRVDPRLMAIARARSDDMVANSYFSHVQPDGRNVFD
                     ILTAQHITWYNAGEIIASNNYPMDSTVSAANRQWLGSPGHYAIITSTDLNYIGVGLAI
                     DPATGKKLWTAVYIKGPDRTSAKATVYTPKVVASPTPTTRRVKVAWTGYDPRLQVLTS
                     GLRSYAIQRRTDGGAWTTVLSSTTARYTYFTLALGHRYEFRISARDRAGNPGAWVTKV
                     IDLR"
     gene            complement(1430..2875)
                     /locus_tag="VHM48_02275"
     CDS             complement(1430..2875)
                     /locus_tag="VHM48_02275"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2754258.1"
                     /translation="MAAPAGLAPPSAKAVVAPEAGAAPEAWEAAPGVANERDPASGPR
                     AVSPAVARRFLALHHFLAQPRSLPTGSAGILAVFERLGSIQFDPIEIAGRNHDLVLLA
                     RVAGYRREMTDQLLYTERALYETYNKGLSIVPTHELPWYRVAWDRARQQHDGEAFDEH
                     APLVAELMDRIRTTGPMSSIDIEPRAAIEWSWRPTNQVRALLEGLAEAGILGLSRRDG
                     NRRVYDLIERLFPAEVLAQVVPVRDRFRHKLLSRYRAHGLLGTSGSAELWLGTSPRID
                     IGMEDGVPLKVAGRRELQRELVADGAILPVVVEGIRGLRYLPATELALLEQAEREIAA
                     GSAPGGERPGVAFLAALDPLVWDREFLRTLYDFDYVWEVYVPAAKRRWGYYVLPILFG
                     DRLVGRIEPRIERKSGTLRIAGLWWEDGFDPVAEPGFVAAFAAAIEAHRAFGGVDRVT
                     WPRATRHRPIVQAVRARSRPGAARRDDPART"
     gene            complement(2910..3425)
                     /locus_tag="VHM48_02280"
     CDS             complement(2910..3425)
                     /locus_tag="VHM48_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2754259.1"
                     /translation="MVLRQSAGILLFRRRGPADAGIEVLLAHPGGPFFAQRDEGHWTI
                     PKGEPDALDEDLLAVARREFAEEVGHPAPSMQPDGTPPIELGTIVQKGGKVVHAWAIE
                     GDLDPAVAISNEFEMEWPPRSGRLQTFPEIDQVGWFDPSEASRRLKPTQAPFVDRLVA
                     RLAGTAKPDGS"
     gene            3588..4157
                     /locus_tag="VHM48_02285"
     CDS             3588..4157
                     /locus_tag="VHM48_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754260.1"
                     /translation="MPDLSMPEIHLPDIKLPEGLRDMNRHDIQNAISERMPKKIEMPD
                     VDLSKIDLPKAIEDRLNKVEKAINKMDLPKAVEDRMPRRKKRANPILPIAALVAIGSM
                     FVAAWWLITSPSASLKVRETADRIKARMTGRETGLQRFDDEAELGSLLPNPDQTRPSP
                     EAETFPDTFAELGETVSAGNGSTSDRASV"
BASE COUNT          658 a         1587 c         1481 g          588 t
ORIGIN      
        1 gtcgcgcctg ggaccgtggc ccggacggtg atcgccgcag cggtcacggt ccacccggcc
       61 tcgagcttgt cggcgctcac atcgacctgg accgccagcg cggtcggcgg cgtgcccgcc
      121 ttggcgagct cgttcgagca ggccatcgag aagcacgaag cgtgggccgc ggcgagcagc
      181 tcctcgggcg acgtgacgcc ttccggctcg ccggtgcgcg acgcccaggt cgtcggctgg
      241 tcgcggaaga tgccggtcgt cgctgcggtc acggtgccat tgccggacag caggtctccg
      301 ctccaggtgg cagttgcgcg tcgggtggtg gccatcgagg ccctccagat aacggtcggg
      361 gatccagcat gagggcgagg cgggcgtcgg gccgcgttcg actcgggctg gctgccacac
      421 cgatggtccc atagcatccg gatccggtcc ggttgggtgg ccaccggttc gggtcggatc
      481 ggtggagacc cctgccgccg atgcagtaga caacagcccg taccaatcgc cccggtcgtc
      541 gggacgcctc acccataccg ccgcggggcc cgctccggga tcgttgcggc atggtcctca
      601 cgctcccctc gctcacccgt cgacgcctcg cgtcactcgc cctcgtcgcc gtcctcggcg
      661 ccgtcatcgc cgcgccgggc gccgcgaccg cgaccgtgac gaccgtcgac gacgcgacgc
      721 tgacggccgc cgaaaccgcg atggtgtcgg cgctcaacgc cgaccggacc gaccgcgggc
      781 tcgttgcggt tcgcgtcgat ccgcgcctga tggcgatcgc ccgggctcgc tcggacgaca
      841 tggtcgcgaa cagctacttc agccacgtcc agcccgacgg ccggaacgtc ttcgacatcc
      901 tgaccgcgca gcacatcacc tggtacaacg cgggcgagat catcgcctcg aacaactacc
      961 cgatggactc gaccgtcagc gccgcgaacc gccagtggct gggctcgccc ggtcactacg
     1021 cgatcatcac gtcgacggat ctcaactaca tcggcgtcgg cctcgcgatc gatccggcga
     1081 ccggcaagaa gctctggacg gcggtctaca tcaagggccc cgaccggacc tcggccaagg
     1141 ccacggtcta caccccgaag gtcgtcgcca gcccgacccc gaccacgcgc cgggtcaagg
     1201 tcgcctggac cggctacgac ccgcgcctcc aggtcctgac gtccggcctc cgctcgtatg
     1261 cgatccagcg ccggaccgac ggtggggcgt ggacgacggt cctgtcgtcg acgaccgcgc
     1321 gctacacgta cttcaccctc gccctcggcc accgctacga gttccggatc tcggcccgcg
     1381 atcgggccgg caatccgggg gcctgggtga cgaaggtcat cgacctccgc taggtccgcg
     1441 cggggtcgtc gcgccgcgca gcccccgggc gcgaacgggc ccgcaccgcc tggacgatcg
     1501 gccgatgccg cgtcgcccgc ggccacgtca cccggtcgac tccgccgaac gcccgatggg
     1561 cctcgatggc tgcagcaaac gcggcgacga accccggctc ggcgaccgga tcgaacccgt
     1621 cctcccacca cagtccggcg atccggaggg tgccggactt ccgctcgatg cgcggctcga
     1681 tccgcccgac gagtcggtct ccgaacagga tggggaggac gtaatagccc cagcgccgct
     1741 tggcggccgg gacgtagacc tcccagacgt agtcgaagtc gtacagggtg cggaggaact
     1801 cgcggtccca gaccaggggg tcgagcgccg cgaggaacgc gacacccggg cgctcaccac
     1861 cgggcgccga cccggccgcg atctcccgct ccgcctgctc gagcagcgcc agttcggtgg
     1921 ccggcagata gcgcagcccg cggatcccct cgacgacgac cggcaggatc gctccgtccg
     1981 cgacgagctc gcgctggagc tcacgccgac cggcgacctt gagcggcacc ccgtcctcca
     2041 tcccgatgtc gatccgcggc gaggtgccaa gccagagctc cgcgctgccc gacgtgccca
     2101 acagcccgtg agcccggtag cgggacagga gcttgtggcg gaaccggtcc cggaccggaa
     2161 cgacctgggc gagcacctcg gccgggaaca gccgctcgat gaggtcgtag acgcggcgat
     2221 tcccgtctcg ccgcgacagg ccgaggatcc ccgcctcggc gaggccctcg aggagcgccc
     2281 ggacctggtt tgtcggtcgc cacgaccact cgatcgcggc ccgcggctcg atgtcgatcg
     2341 acgacatcgg cccggtcgtg cggatccggt ccatgagctc ggcgaccagc ggagcgtgct
     2401 cgtcgaacgc ttcgccgtcg tgctgctggc gggcccggtc ccatgcgacg cgataccacg
     2461 ggagctcgtg ggtcggcacg atcgagagcc ctttgttgta ggtctcgtac agagcccgct
     2521 cggtgtacag cagctggtcg gtcatctcgc ggcggtagcc ggcgaccctg gccagcagga
     2581 cgaggtcgtg gttccggccg gcgatctcga tcgggtcgaa ctggatcgac ccgaggcgct
     2641 cgaagaccgc gaggatgccg gccgatccgg tcggcaggga gcgcggctgc gcgaggaagt
     2701 gatggagcgc gaggaatcgc cgggcgacag ccgggctgac ggcgcgcggc cccgatgccg
     2761 gatcgcgttc gttcgccacg cccggcgcgg cctcccaggc ctcgggcgcc gcaccggcct
     2821 cgggcgccac cacggccttc gcggagggcg gggcaaggcc ggcaggtgct gccatcggtg
     2881 tcaccggccc atcgtcgccg ccgatggcgt caggacccgt caggtttggc ggtgccggcg
     2941 agtcgcgcga ccagccgatc gacgaacggg gcctgggtcg gcttcagccg gcgagatgcc
     3001 tcggacggat cgaaccagcc cacctgatcg atctccggga aggtctggag ccggccgctt
     3061 cgcggcggcc actccatctc gaactcgttc gagatggcga cggccggatc gaggtcgccc
     3121 tcgatcgccc acgcgtggac gaccttgccg cccttctgga cgatcgtccc gagctcgatc
     3181 ggcggcgttc cgtcgggctg catcgacggc gccggatggc cgacctcctc ggcgaactcc
     3241 cgacgggcga cggcgaggag atcctcgtcg agcgcgtcgg gctcgccctt ggggatcgtc
     3301 caatggccct cgtcgcgctg ggcgaagaac ggtccgccgg ggtgggcgag gaggacctcg
     3361 attcccgcgt cggccgggcc ccgccgacgg aacaggagga tgccggcgct ctgcctcagg
     3421 accatccgcg aatgctagcc gagcggagcc ccgccccacg attgcccgcc cggggaccac
     3481 ccgacaccgc tgtgatgcac ctgcaacccg ctggtggctg gcggcggtcg gcccgtcacg
     3541 gtcgcgaccg catcgttggg gacagcactc acccggaagg agaccccatg cccgatctct
     3601 ccatgccgga gatccacctg ccggacatca agctgccgga aggactccgc gacatgaacc
     3661 gtcacgacat ccagaacgcc atcagcgagc gcatgccgaa gaagatcgag atgcccgacg
     3721 tggacctctc gaagatcgat ctgccgaagg ccatcgagga ccgcctgaac aaggtcgaga
     3781 aggccatcaa caagatggac ctgccgaagg ccgtcgagga ccggatgccc cgccgcaaga
     3841 agcgggcgaa cccgatcctg ccgatcgccg cgctcgtcgc catcggctcg atgttcgtcg
     3901 ccgcgtggtg gctgatcacc tcgccgagcg cctcgctcaa ggttcgcgag accgccgacc
     3961 ggatcaaggc ccggatgacg ggccgggaga ccggtctcca gcgcttcgac gatgaggccg
     4021 agctcggcag cctgctcccg aaccccgacc agactcgacc gtcccccgag gccgagacct
     4081 ttcccgacac cttcgccgag ctcggcgaga ccgtcagcgc aggtaatggc tcgacgagcg
     4141 atcgggcgag tgtctgaagc cccgtgtccg aagcccttct gaaccgggcc ggtctgggtc
     4201 ggcacgcgct tcgcgcggaa cgacccggcc gtgcgccggg tcgttcgctt ggcggcgggc
     4261 gacgcgacgg cccgcaactc cgccgcggac cggcggtccg ctcaccctgg agac
//
LOCUS       DATAIW010000071         4307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_62801, whole genome shotgun sequence.
ACCESSION   DATAIW010000071 DATAIW010000000
VERSION     DATAIW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62801"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VHM48_02290"
     rRNA            <1..329
                     /locus_tag="VHM48_02290"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            479..595
                     /gene="rrf"
                     /locus_tag="VHM48_02295"
     rRNA            479..595
                     /gene="rrf"
                     /locus_tag="VHM48_02295"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            906..1502
                     /locus_tag="VHM48_02300"
     CDS             906..1502
                     /locus_tag="VHM48_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX2754261.1"
                     /translation="MVDPPAPQPNDKQRRHCLSVDHVECPMYRAARAARTTTLAGGAD
                     PELIEAADRRRRPLARTAPILLESPRLVDKAVRLQFERAPGQLALIALMVVAFAIVAL
                     TRLSAGGAPAASTSPGPSTTVASPSPTSRPTVVATSSVGPSVSPSPVPSVRATYRVKK
                     GDTLAGIAKHFGTTAAKIKAFNGLTSSTLKIGQLLKIP"
     gene            complement(1622..2497)
                     /locus_tag="VHM48_02305"
     CDS             complement(1622..2497)
                     /locus_tag="VHM48_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2754262.1"
                     /translation="MPELPDLAIVADAFHAALAGRQVTAARALAPLAVRGTPAELEAL
                     VGQRLVRIRRRGKLLIVDLDRDRVVINPMLTGRLQLAPPAAALPTKTAVMLTFGPRAR
                     RPRDAAKWTTRATWIPADDADTEIRYRDPTQMGKVYLVPAGVVRAVPGLTDDELGPDA
                     DDPALTLDVWRARISRHPGELKNLLKNQAFVAGIGNAYSDEILHAAGLLPFRKRSSLA
                     DEEIDELYAATRSTLATAITVLRERVPPTFEKQARDWLAVHDKGGHACPRCGTRISEV
                     TPGGFVTSFCRGCQH"
     gene            2710..>4307
                     /gene="rpsA"
                     /locus_tag="VHM48_02310"
     CDS             2710..>4307
                     /gene="rpsA"
                     /locus_tag="VHM48_02310"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX2754263.1"
                     /translation="MTDEQQDGPAQAVASSPDETPDRAAAATAVADEPATADVAATTA
                     ADDAPQTGDDAPAAEASAAEVTEPAAGAPDDAPEAESVATDAPAIEAVTDADNGTGAV
                     AEAGTDSTLSADDMVDAPAAEEEDPADLVPAPRDLGPEPTTMEELLAEQDSDIKSFKH
                     GDVVEGTVVRIDKDEILVDIGAKSEGVVSNRELYGRHAESQPQLAIGDTVLVYVLQPE
                     SQEGHAVLSLRRAGLERKWRSMQEQFEAGVIIEAPVIDHNKGGLIVDCGIRGFVPISQ
                     IVDFPRRPQNDQPRDAAQEIAEKLMPFVGRKLRLKILEVNRKANRLILSEKVALYEER
                     REKRDELFSSLQVGQKVSGTVRSIAPFGVFIDLGGIDGLVHKSELSWNKVNNPESGYK
                     VGDEVEAEVIDINHERGRISLSIRRLQPDPWHSTVADFNVGDVIDGTVTKLVNFGAFV
                     RVRDGLEGLIHISELSHQRVAHPGDVVHEGQTLKLKIISLDSERHRLGLSLKQAEEPP
                     ARPAATEAPRPRRPREERGYAMSDA"
BASE COUNT          722 a         1470 c         1457 g          658 t
ORIGIN      
        1 cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc tctatccgcc gtgggcgtag
       61 ggaacttgag aggagttgct cctagtacga gaggaccgga gtgaacgaac cgctggtgtg
      121 ccagttgtcc cgccaggggc accgctgggt agctatgttc ggtcgggata agcgctgaaa
      181 gcatctaagt gcgaagctcg cctcgagatg aggttcccca ccgggtcaac cgggtaagac
      241 cgcagggaga ccacctgttt gataggccgc atgtggaagt tcggtgacgg atgaagctga
      301 gcggtactaa cggtcgaggg cttgacctct acagcaactg acctggggtc agtggctggg
      361 acatcgtgga tcgatccacg tcttgacatt gatttcgaag atcgattgca gaacggcgcc
      421 cacgccggcc ttcgggctcg cgctggtcgc agccgaacaa catccagacg caacgaatcc
      481 tggtgacact ggcggagagg ccatacccgt tcccatcccg aacacggaag tcaagctctc
      541 cagcgccgaa gatactgaga gggcagcctt tcgggaaaat aggtcgtcgc caggattttt
      601 gcgtttccgg gacctgcgtt gccgacggcc ctggtcgacg cacgccggtc agctcgttgc
      661 cggtgacggt cgagcggcga tcacggtcgc cggccggtag actggcgtcg atgaccgttc
      721 ccgtcagggc cgaggacgcg ttggagcagc ggccgggggc gatgcacgac gtgcctgccg
      781 atccgggccc gcgcctccgg ccccctccct ccgctcgtga tgccgtcgct ggcacgtgcc
      841 cttacctgac gtcgtcgggc ggttcgtggc gactcgccag cccgtcccgg cagcatcgct
      901 gcgcggtggt cgatccgccg gccccgcagc cgaacgacaa gcagcgccgg cactgcctgt
      961 cggtcgatca cgtcgaatgc ccgatgtacc gggccgcccg cgctgcgcgg acgacgaccc
     1021 tcgccggcgg cgccgatccc gagctgatcg aagctgccga ccgtcgccgc cgaccgcttg
     1081 cccggaccgc gccgatcctg ctcgaatccc cccgcctggt ggacaaggcc gtcaggctcc
     1141 agttcgagcg agcgcccggc cagcttgcgc tgatcgcgct gatggtggtc gcgttcgcga
     1201 tcgtggccct cacccgcctg tcggctggcg gcgcgccggc cgcatcgacg tcgccgggac
     1261 cgtcgaccac cgtcgcgtct ccgagtccga cctcgaggcc gaccgtggtc gccacgtcgt
     1321 cggtcggccc gtccgtctcg cccagcccgg tcccgtccgt tcgcgcgacc taccgggtga
     1381 agaagggcga cacgctggcc gggatcgcca agcatttcgg aaccacggcc gcgaagatca
     1441 aggcattcaa cgggctgacg agctcgacgc tgaagatcgg ccaactcctg aagatcccct
     1501 gaacaccgcg gtcggtgggt cgcgatacgt cgcgggagct cgacgagttg agctccgcct
     1561 cggaccgcgg cgaccgattc gcgggcgatc ggggcgagtg ccactctggg tgcgccaacc
     1621 ttcaatgttg gcagcctcga cagaacgagg tgacgaagcc acccggcgtg acctcgctga
     1681 tccgcgtccc gcagcgcggg cacgcgtggc cgcctttgtc gtggaccgcg agccagtcgc
     1741 gtgcctgttt ctcgaacgtc ggcggcacgc gttcgcgcag gaccgtgatc gccgtggcga
     1801 gcgtgctgcg ggttgcggcg tagagttcat cgatctcctc gtcggcgagc gacgagcgct
     1861 tccggaacgg caggagccca gcggcatgga ggatctcgtc gctgtaggcg ttgccgatcc
     1921 cggccacgaa ggcctggttc ttcaacaggt tcttgagctc gccgggatga cgactgatcc
     1981 tggcccgcca gacgtcgagg gtcagggcgg gatcgtccgc atccggtccg agctcatcgt
     2041 cggtcaggcc cgggaccgcg cgaacgacgc ccgcgggaac gaggtaaacc ttgcccatct
     2101 gggtcggatc gcgatagcgg atctcggtgt ccgcgtcgtc ggccggaatc caggttgccc
     2161 gggtcgtcca cttggcggca tccctcggcc gcctcgcgcg cggtccgaac gtgagcatca
     2221 cggcggtctt cgtcggcaga gcggcggccg gcggcgccag ctggaggcgc ccggtcagca
     2281 tcggattgat gacgacacga tcgcgatcga gatcgacgat caggagcttg ccccgccgtc
     2341 tgatgcggac gagccgttgg ccgacgaggg cctcgagctc ggccggcgtg ccgcgcacgg
     2401 cgagtggcgc aagggctcgg gcggccgtca cttgtcggcc ggccagggca gcgtggaacg
     2461 cgtcggcgac gatcgccagg tcggggagtt cgggcacggc ggatcgattg tagccgcgta
     2521 aaccgctgtg ctagactctc ccggccctag aaggcagaca gctgaagacg tgtccagcca
     2581 gctcgccacg aagggagcgg accgcggccc atctaccacg gaccacctct gatcgttccg
     2641 gacccggctt gggcgctgcc caggccgttg ccgtgtctgg ggacttgacc gacaggagag
     2701 accagacact tgaccgacga acagcaggac gggcccgccc aggccgtcgc ctcgagccct
     2761 gacgagaccc ccgaccgggc cgcagccgcg acagccgttg ccgacgagcc cgccaccgcc
     2821 gatgtcgccg cgacgaccgc ggcggatgac gctccccaga ccggcgacga cgctcccgcg
     2881 gccgaggcat cggcagcaga ggtgactgag ccggctgccg gcgcacccga cgatgcccct
     2941 gaagccgaat cggtcgcgac cgatgccccg gcgatcgagg ccgtgacgga tgccgacaac
     3001 gggacgggcg cggtcgcgga ggccggcacc gactcgacgc tcagcgctga cgacatggtc
     3061 gatgcgcctg ccgccgagga ggaagatccg gcggacctcg tgcccgcccc ccgcgatctc
     3121 ggccccgagc cgacgacgat ggaggagttg ctcgccgagc aggactccga catcaagagc
     3181 ttcaagcatg gtgatgtcgt cgaaggaacc gtcgtccgga tcgacaagga cgagatcctc
     3241 gtcgacatcg gtgcgaagtc cgagggcgtc gtcagcaacc gtgagctcta cggtcgccat
     3301 gccgagtcgc agccgcagct cgcgatcggc gacaccgttc tcgtctacgt cctccagccg
     3361 gagtcgcagg agggccacgc cgtcctgtca ctgcggcggg ccggcctcga gcgcaagtgg
     3421 cgctcgatgc aggagcagtt cgaggcgggc gtcatcatcg aagccccggt catcgaccac
     3481 aacaagggtg gcttgatcgt cgactgcggc atccgcgggt tcgtgccgat cagccagatc
     3541 gtcgacttcc cacgccggcc gcagaacgac cagccgcgcg acgccgccca ggagatcgcc
     3601 gagaagctga tgccgttcgt cggccgcaag cttcgcctca agatcctcga ggtcaaccgc
     3661 aaggcgaacc gcctgatcct gtccgagaag gtcgccctct acgaagagcg gcgtgagaag
     3721 cgggatgagc tgttcagcag cctccaggtc ggccagaagg tcagcggcac cgtccgctcg
     3781 atcgccccgt tcggcgtctt catcgatctc ggcggcatcg acggcctcgt ccacaagagc
     3841 gagctgtcgt ggaacaaggt caacaacccc gaatcgggct acaaggtcgg tgacgaggtc
     3901 gaggccgagg tcatcgacat caaccacgag cgtggccgga tctccctgtc gatccggcgc
     3961 ctccagccgg atccgtggca ctccacggtc gccgacttca acgtgggcga cgtgatcgac
     4021 ggaacggtga ccaagctcgt caacttcggc gccttcgtcc gtgttcgcga cggcctcgag
     4081 ggcctgatcc acatcagcga gctgtcccat cagcgcgtcg cccatcccgg cgacgtcgtc
     4141 cacgagggcc agactctcaa gctcaagatc atctcgctcg actccgagcg gcatcgtctc
     4201 ggcctcagcc tcaagcaggc cgaggagccg ccggctcgtc cggcggcgac cgaggcaccg
     4261 cggccacgac gtccccgcga ggagcgcggc tacgcgatgt ccgatgc
//
LOCUS       DATAIW010000072         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_33015, whole genome shotgun sequence.
ACCESSION   DATAIW010000072 DATAIW010000000
VERSION     DATAIW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33015"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="ilvC"
                     /locus_tag="VHM48_02315"
     CDS             <1..849
                     /gene="ilvC"
                     /locus_tag="VHM48_02315"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2754264.1"
                     /translation="IMIAVPDTLQKSVYDAEIEPYLRPGQLLMFAHGFNIRFGRISPP
                     DTIDVGMVAPKGPGHLLRSVYEAGGGVPALFAVENDASGTARERVLAYARGIGSTRAG
                     VLETTFKEETETDLFGEQALLCGGVSALIKAAFETLVEAGYQPELAYFETMHELKLIV
                     DLMYRGGLNFMRFSVSDTAEYGDYVSGPRITEGVKTAMKDVLSDIQSGSFASRWIAEQ
                     ESGGAEFARLRQQDKEHQIEQVGRDLRAQMAFLNPVVVEAGEAQGSVGAGATGSPAPA
                     AAGTTR"
     gene            846..2465
                     /locus_tag="VHM48_02320"
     CDS             846..2465
                     /locus_tag="VHM48_02320"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX2754265.1"
                     /translation="MSGSAVAAGTVRIFDTTLRDGEQAPGAGLTAAEKLEVARQLARL
                     KVDVIEAGFPAASPGDFEAVHRIAQETKGGIAVAALARCRDGDPQRAVEAIRIAERPH
                     LHVFIATSDIHLKHKLRISREEALAEAVRWVRFGREALGADAEIEFSAEDASRTDTDF
                     LLQVYESVVAAGASTVNIPDTVGYAIPSEFGVLVGRVVDLVGRDATVSVHCHNDLGLA
                     TANTLAAVQAGARQVEVTINGLGERAGNASLEEVVMGLRTRPTQFPDLVSGVQTEQIT
                     AASRLVSYLTGFAVQPNKAIVGGNAFAHESGIHQDGVIKNPLTYEIMTPQSVGLTGSQ
                     LTIGKLSGRRGLQGKLRELGHDVEGEALDTIYRQAIALADMKKEVTDGDLLALVEQRA
                     SEVPASVELIGWSVTSSHGGNATGSVTLTVNGEDRSAEATGNGPVDALYSAVDEALHG
                     ILGWHPVLTEWEIKAVSAGEDAQGQVLCRCRRSSDEGPGALVVSGHGLSTNIIEASLD
                     GYLAAVNKLHGADINGVDVAFIGRRSAEDLP"
     gene            2462..>2911
                     /locus_tag="VHM48_02325"
     CDS             2462..>2911
                     /locus_tag="VHM48_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2754266.1"
                     /translation="MTEAHYRIATIPGDGVGPDVVAAARAAVDAAGSAFGFAVDWSEH
                     LAGGVAIDTHGVAIRPADVEACGAADAILLGAVGGPKWSDPSAPVRPEQALFALRGGL
                     GLYANLRPVSVHPALIASSPVRPELLAGVDLLIVRELTGGIYFGERRE"
BASE COUNT          425 a         1061 c         1014 g          411 t
ORIGIN      
        1 atcatgatcg cggtcccgga cacgcttcag aagtcggtct acgacgccga gatcgagccg
       61 tatctgcggc ccggccagct cctgatgttc gcccacggct tcaacatccg gttcggccgg
      121 atcagcccgc ccgacacgat cgatgtcggg atggtcgccc ccaagggccc gggccatctc
      181 ctccgctcgg tctacgaggc gggcggcggc gtgccggccc tgttcgccgt cgagaacgac
      241 gcctcgggca ccgcccgcga gcgtgtcctc gcctacgccc gcgggatcgg ctcgacccgg
      301 gcgggcgtcc tcgagacgac cttcaaggag gagaccgaga ccgacctgtt cggcgagcag
      361 gcactcctct gcggcggcgt gtcggccctg atcaaggcgg cgttcgagac gctcgtcgag
      421 gccggctatc agcccgagct cgcctacttc gagacgatgc acgagctcaa gctgatcgtc
      481 gacctgatgt accgcggcgg cctgaacttc atgcgcttca gcgtcagcga caccgccgag
      541 tacggcgatt acgtctcggg cccgcggatc accgagggcg tcaagacggc gatgaaggac
      601 gtcctctcgg acatccagag cggctccttc gccagccgct ggatcgccga gcaggagtcg
      661 ggcggggccg agttcgcgcg gcttcgccag caggacaagg agcaccagat cgagcaggtc
      721 gggcgcgacc tccgcgccca gatggcgttc ctcaacccgg tggtcgtcga ggccggcgag
      781 gcccagggtt cggtgggtgc aggcgccacg gggtcgcccg ctccggcggc cgcaggcacg
      841 acccgatgag cggctcggcg gtcgccgccg gcaccgtccg gatcttcgac acgacccttc
      901 gggacggcga gcaggcgccg ggggccggac tgaccgccgc cgagaagctc gaggtcgccc
      961 gccagctcgc ccgactcaag gtcgacgtca tcgaggccgg ctttccggcc gcctcgcccg
     1021 gcgacttcga agccgtccac cggattgccc aggagacgaa gggcgggatc gccgtcgccg
     1081 ccctcgcccg ttgccgcgac ggcgacccgc agcgcgcggt cgaggcgatc cggatcgccg
     1141 agcggccgca cctccacgtc ttcatcgcca cgagcgacat ccacctcaag cacaagctcc
     1201 ggatctcgcg cgaggaagcc cttgccgaag ccgtccgctg ggttcgcttt ggccgggagg
     1261 cgctcggtgc cgacgccgag atcgagttca gcgccgagga cgcctcgcgg accgacaccg
     1321 actttctcct ccaggtctac gagtcggtcg tggccgccgg cgcctcgacc gtcaacatcc
     1381 ccgacaccgt cggctacgcg atcccgagcg agttcggcgt gctggtcggc cgcgtcgtcg
     1441 atctcgtcgg gcgcgacgcg acggtcagcg tccattgcca caacgacctc gggctcgcca
     1501 cggccaacac cctggccgcc gtccaggccg gcgcccgcca ggtcgaggtc acgatcaacg
     1561 ggctcggcga gcgggccggg aacgcctcgc tcgaggaggt cgtcatgggc ctccggacgc
     1621 gcccgaccca gttcccggat ctcgtcagcg gcgtccagac ggagcagatc acggcggcgt
     1681 cgcggctcgt gagctacctg accgggttcg ccgtccagcc gaacaaggcg atcgtcggcg
     1741 gcaacgcctt cgctcatgag tcggggatcc accaggacgg cgtgatcaag aacccgctga
     1801 cctacgagat catgaccccg cagtcggtcg ggctgaccgg cagccagctg acgatcggca
     1861 agctgtcggg ccggcgcggc ctccagggca agctccgcga gctcggccac gacgtcgagg
     1921 gcgaggcgct cgacacgatc taccgccagg cgatcgccct cgccgacatg aagaaggagg
     1981 tcaccgacgg cgacctcctc gccctcgtcg agcagcgggc gtcggaagtc ccggcgtcgg
     2041 tcgagctgat cggctggagc gtcacctcgt cgcacggcgg caacgccacc ggcagtgtga
     2101 cgctgacggt gaacggcgag gaccgctcgg ccgaggcgac cggcaacggg ccggtcgacg
     2161 cactctattc ggccgtcgac gaggcgctcc acgggatcct cggctggcac cccgtcctga
     2221 ccgagtggga gatcaaggcg gtctcggccg gcgaggacgc ccagggccag gtcctgtgcc
     2281 gctgtcgtcg ttcgtcggac gagggcccgg gcgccctcgt ggtctccggc cacgggctgt
     2341 cgacgaacat catcgaggcc tcgctcgacg gctacctcgc tgccgtcaac aagctccacg
     2401 gggccgacat caacggcgtc gatgtcgcgt tcatcggccg gcgcagcgcg gaagacttgc
     2461 cgtgacggag gctcattacc ggatcgcgac gattcccggc gacggggtcg ggccggacgt
     2521 ggtcgccgcg gcgcgggccg ccgtcgatgc ggccgggtcg gccttcggct tcgccgtcga
     2581 ctggtcggag catctcgcgg gcggggtggc gatcgacacc cacggcgtcg cgatccggcc
     2641 ggcggacgtc gaggcctgcg gcgccgccga cgcgatcctc ctcggcgccg tcggcggtcc
     2701 gaaatggtcc gatccgtcgg cgccggtccg gccggagcaa gcgctgttcg ccctgcgcgg
     2761 cggtctcggc ctgtatgcca atctccgccc ggtgagcgtc catccggcgc tgatcgcctc
     2821 gtcgccggtc cgcccggagc tgctcgccgg cgtcgacctc ctgatcgtcc gcgagctcac
     2881 cggcggcatc tacttcggcg aacggcgcga g
//
LOCUS       DATAIW010000073         7539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_239743, whole genome shotgun sequence.
ACCESSION   DATAIW010000073 DATAIW010000000
VERSION     DATAIW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239743"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..746
                     /locus_tag="VHM48_02330"
     CDS             237..746
                     /locus_tag="VHM48_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754267.1"
                     /translation="MAQPQPSPEFRVTRLIYRLNSETVTDDFGRAEPLDGELDGWAFR
                     LEDGILSATPSEEFRSRESAKVHLDARLCEWSQSAFLSGSRFQIRFDFERSDVEAIDP
                     PPNHVFVFPEAAPATVSMGTPTIVLGHRAYPAPDPAFRRTPLTDLLTERLRALEDGRA
                     ELPAWPIWS"
     gene            complement(1031..1459)
                     /locus_tag="VHM48_02335"
     CDS             complement(1031..1459)
                     /locus_tag="VHM48_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754268.1"
                     /translation="MSRAALYNAGKHGLALTPTDIGFQMDDGSLLKVEGPAIQYLEVR
                     DDEAGRPRWEQVTHWVRPDRQIGLIYRTCEMIEALWQTARYRYLGPKETGGYKFHLVS
                     GPTYHDLVFSDAPETGVVIDDMRWQLAYDDDPDAQARQVD"
     gene            complement(2178..3893)
                     /locus_tag="VHM48_02340"
     CDS             complement(2178..3893)
                     /locus_tag="VHM48_02340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2754269.1"
                     /translation="MTALLDAFGRRHPAAGGLVPGLNPTERVASLDGATFDAVVIGAG
                     ILGAGTARELARRGLRTLVVDRRDVGWGTTNRSTRLVHGGLRYLEHFDFPLVHEGLRE
                     RAWLLRAAPHLVTPLGFLLPFYREPWWRRTELRLGLTLYDLLSPRQSLPRHRALSAAR
                     AARLEPALSPTGLRSAVLYWDGQVELPERLVVEILRAAEQFGATVVTHCRAVDLRLVD
                     GRVDGVTLAADGGLAAVGVPDSGPAGLPGSSATAEVRAPLVVNASGPWADQVLATGGV
                     RRPPLLRLTQGIHLRYPLRTRHAVAFEHPIDGRLCFSIPWQGGTMVGTTDTDVEGGPE
                     QARVSAADLAYVDDGARFLFPDVARLRPTWAEVGVRSLMRKDGSAGAVSRKHVVLDHG
                     QDGAGGLTTVAGGKLTAWRSIAADVVDRVVGRRDPTALGAVGFRGEPLPDRRRRGSAD
                     EASGVLPATSDERLDSLYGPYRGEVAAVAAEDPWWAEPILPGYPAIRAEIAQAVRNEW
                     SATAADIVLRRLVLGFDQTVAREAGAAVAAVLRERLGWSEARLAADLDDLARELREHE
                     VAMPV"
     gene            complement(3890..4579)
                     /locus_tag="VHM48_02345"
     CDS             complement(3890..4579)
                     /locus_tag="VHM48_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754270.1"
                     /translation="MAEFIFMLTHNDETVANALDVYDVLRDTELRHVGFKDVGASPEA
                     LAELARRMHEDGRIVYLEVVSVSADDELRSIRVARDIGVDVVMGGTHVDEAIELLAGS
                     GLRYFPFPGRIIGHPSVLDGTIDEIAASAADLSNHDGVHGLDLLAYRHTGPVEPIIDA
                     VVRASRGPVVVAGSIVSEAQIRALTDLGAWGFTIGGAAFEQALPAETTIRAQVEWALE
                     TAARATAERPS"
     gene            complement(4591..5478)
                     /locus_tag="VHM48_02350"
     CDS             complement(4591..5478)
                     /locus_tag="VHM48_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX2754271.1"
                     /translation="MIGDGEGDGPLARVERRYAGYVFDLDGTLYLGDALLPGARDAVL
                     ALRAAGSRTVFLTNKPLERPADYAAKLSRLGIPAISADVVSSTDALLRYLGLHASGAR
                     LYPVAEPLLWDLLEEAGWELTDDPARIDVVVVSFDRTFAYSKLQIAFDAVRAGARIVA
                     TNPDAWCPVPGGGLPDCAAMLAAIEACTGARAEAIVGKPSAHMAATLLDRLALVADHT
                     LLVGDRLATDMRMAIEAGMAAALVLSGATTLADVAAGPVRPRYVLAGVADVVPIDLAR
                     AAAQPAGLPLAPRPPVPDP"
     gene            complement(5475..6980)
                     /locus_tag="VHM48_02355"
     CDS             complement(5475..6980)
                     /locus_tag="VHM48_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2754272.1"
                     /translation="MPDRSPDERPASIGADAAERDVSPTAAAERPMPDPTDLVALGTM
                     IGDADPAGRLHPIGMRTIRIGEDALAEVPVIVRALARPGPVVVLVDSTPMQRGPDDLK
                     SLVVELLEAVGEVRLAVIGRGRAELHADDAALAEADSAIEGAGCVVSVGSGTITDIAK
                     DATHRAGDVPLVAIQTAVSVNAFSDDMAVLVRSGVKRTMPSRWPDALVIDLAVIADAP
                     QDMNRAGFGELASMFTAPADWYLAGALGMDADWHPEPVALFRREGEALLAAADAVQTN
                     DPGALALLARLMTLSGIALGVAGKTAPISGTEHIVSHLLDMDAEQAGRSLAFHGAQVG
                     VAAIVVALAWTETLDTFEPATVDVDAAYPAAAIVEAEVRAAFAALDPSGAVANECWRD
                     VEHKLTRWSDARPAFERFLADWPRHRAALRRLVVDPAVLAGAVRRAGAPARFAELSPP
                     VAPALARWALLNCHRMRDRFVLPDLRASCSTWTPPDIDRLLDRAAALGAGL"
     gene            complement(6973..>7539)
                     /locus_tag="VHM48_02360"
     CDS             complement(6973..>7539)
                     /locus_tag="VHM48_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2754273.1"
                     /translation="TIGRSSFDELCAAAALVPAGSDGVIFVPALSGAMTPRWNGRARA
                     VFHGLSMAHGLGHLARAVFEGCTFGLRDIVDRFAELGLGEGEIRVVGGGSRSDLLLQM
                     KADVTGKPVRRVVHPEATALGATMIGAVVAGTFASLDEAATALVGLEDRVYEPDPGVF
                     PAYEDAYRTYRALYDAIEPLFDREPVHA"
BASE COUNT         1162 a         2606 c         2729 g         1042 t
ORIGIN      
        1 tatcggtcgt ggaagcggca cggatgaacg ccctcggcca ccgcggcctg ggcaagtcgg
       61 accgatcgac cgacctcgga gccgacgccc gccgaccgtt gaccatcaag cagatcaacg
      121 aaatctcggc gtggcgcacc agccctcacc gttagagatg tgtcgctata tctcggaggc
      181 tgttcagact agaacttggc gagggagcga gcggaagggt gtcaggatgg ataggcatgg
      241 cccaacccca accgtcgccc gagttccgtg tcacccgtct catctatcgc ctgaacagcg
      301 agaccgtgac cgacgacttc ggccgcgccg agccgctgga tggagaactc gacggatggg
      361 ccttccggct ggaagacggg atcctatcgg cgacgccaag cgaggagttc cgcagcaggg
      421 agtcggcgaa ggtccacctc gatgcacgct tgtgcgagtg gtcacagtct gcgtttctgt
      481 ccggcagccg gtttcagatc cgcttcgact tcgagcgatc ggatgtcgag gcgattgacc
      541 cgccgcccaa ccacgtcttt gtgttccctg aggcggcccc ggcgacggtc agcatgggca
      601 cgccaacaat cgtgctcggc catcgcgcct accctgcgcc cgatcctgcg ttccgacgaa
      661 cgccgctcac cgacctcctg actgagcgac ttcgcgcgct cgaggatggc cgcgccgaac
      721 tgccggcctg gcctatctgg tcctgagtgc gcttgagcag gccttcggcg gccggagaga
      781 ctgtgcgacg gcgcttgaca tcgatcccaa agtcctcgac aacctcgggc gcctgtccgc
      841 ccagttcgac ccgcagatcg gtcgaaaggc ccagggagac ccgaagccgc taaccgggcc
      901 cgaactggct tggctcaagg gagtggccat gcgcctcgtt cgacgtgttg gtgagcacag
      961 ggcgggagga ccgttgcccc agatcacgat gactggacta ccgggactcg accccgcctc
     1021 gggctgatcg tcagtcgact tggcgtgctt gcgcgtcggg atcgtcgtcg taggccagtt
     1081 gccatcgcat gtcgtcgatc acgacgccgg tctccggcgc atccgagaac acgaggtcgt
     1141 ggtaggtagg ccccgacacc aggtggaact tgtagcctcc ggtctccttc ggtccgaggt
     1201 accgatagcg cgcggtctgc cagagcgcct cgatcatctc gcacgttcga tagatgagtc
     1261 ctatctggcg gtcgggtctg acccagtggg ttacttgctc ccagcgcggc cgcccggcct
     1321 catcgtcgcg aacctcaagg tactgaatcg ccggcccttc caccttgagg agcgacccgt
     1381 catccatctg gaacccgatg tcggtcggag tcagagccaa gccgtgcttt ccggcgttgt
     1441 agagcgccgc gcgactcaag tgctggtggg cgaaggcccg aaggtaacgc cgcgcccttc
     1501 cgggcgcgtg cttgtacgga cctggccatg tggccacctc actggtggat tcgggtcccg
     1561 cggggtctcc cacgtcgctc ccgataggca aatggtctcg ccgtcgggtc cctggcgcaa
     1621 ccgacggcgg cccatccgat ggtcccctcc gccggccggg acgattggcg agccaggccg
     1681 ctggcggtgc tggagccctg tgcggccgcc agtgaagacg acgagaacga gccgcgggcg
     1741 cgccctgccg gcgaccactg gcccggcatg tagtcgggtc tacggggatg tcgcgagcgt
     1801 ggccaacgtt tcacggtgag cgtcgcgcaa cgtgggcgac ccttgactgc gcctctgctc
     1861 ctggcgagtc tcagccatgc ttcagcgact cgcggccgtc cgctcggccg tcggggcggg
     1921 tcgatgatcg tcgtgaggac gctcggttgt gggtcgcgcc gaaggccaag gcccgtcttc
     1981 gagataaccg cccacttgcg ggcggttatc gccatctgac gtcccgtgat tactgcggtc
     2041 ttacgttccc accgtgagcg attaggtgat gacggcggtt ttcaagaccg tcgcaatcga
     2101 ccgctctgcc acctctcccg cgggcatctt acgaggagtc tggcacgagc cgtcgtcgcc
     2161 ggccgccaac gctggaatca gacgggcatg gcgacctcgt gctcgcgcag ctcccgggcg
     2221 aggtcgtcga ggtccgccgc gagccgcgct tccgaccagc cgagccgctc gcggagaacg
     2281 gccgcgacag cagcgcccgc ctcccgggcc acggtctgat cgaaaccgag caccaaccgg
     2341 cgcaggacga tgtcggcggc cgtcgccgac cattcgttcc ggaccgcctg ggcgatctcg
     2401 gcccggatcg cggggtagcc gggcaggatc ggctcagccc accagggatc ctcggcagca
     2461 accgcggcga cctcaccgcg atagggtccg tacagcgagt cgaggcgctc gtccgacgtg
     2521 gccggcagga cccccgatgc ctcgtcagcc gagccgcgac ggcggcgatc cgggagcggc
     2581 tcgccgcgga aaccgaccgc gccgagggcg gtggggtcgc gccgtccgac cactcggtcc
     2641 acgacgtcgg cagcgatcga tcgccacgcc gtgagcttcc ccccggcgac ggtggtcagg
     2701 ccccctgcgc catcctgccc gtgatcgagg acgacatgct tccggctgac cgctccggcc
     2761 gacccgtcct tccgcatcaa cgatcggaca ccgacctcgg cccaggtcgg gcgtagccgg
     2821 gccacgtcgg gaaacaggaa ccgtgcgccg tcgtcgacgt acgccaggtc cgcggccgac
     2881 acccgggcct gctccggccc gccctccaca tcggtgtcgg tcgtcccgac catcgttccg
     2941 ccttgccacg ggatcgagaa gcacagccgt ccgtcgatgg gatgctcgaa ggcgaccgcg
     3001 tgccgcgtcc ggagcggata gcggaggtgg atgccctggg tcagccgcag cagcggcggg
     3061 cgtcggacgc cgccggtggc caggacctgg tcagcccacg gtcccgacgc gttgaccaca
     3121 agcggcgccc ggacctcggc cgtggccgac gacccgggca acccggcggg cccggaatcg
     3181 ggcactccga ccgctgcgag tccgccgtcg gcggcgagcg tcacgccgtc gacgcgacca
     3241 tcgacgaggc gcaggtcgac ggctcgacag tgggtgacga cggtcgcccc gaactgttcg
     3301 gcggcccgca ggatctcgac gacgagccgt tcgggcaact cgacctggcc atcccagtag
     3361 agcacggcgg atcgcaaccc cgtcggcgac agcgcgggct cgaggcgagc ggcgcgggcc
     3421 gccgagagcg cccggtgacg tggcagcgac tggcgtggcg agaggagatc gtagagggtc
     3481 agcccgagcc ggagctccgt ccgtcgccac catggctccc ggtagaacgg aagcaggaac
     3541 ccgagcggag tgacgaggtg tggcgccgca cgcaggagcc aggcccgttc gcggaggccc
     3601 tcatggacga gcgggaagtc gaaatgctcg agataccgca atccgccgtg aacgagccgg
     3661 gtcgagcggt tggtcgtgcc ccagccgacg tcgcgccggt cgacgaccag cgtccgcagt
     3721 ccccgccggg ccaactcgcg ggcggtcccg gcgccgagga tgccggcgcc gatgacgacc
     3781 gcgtcgaagg tcgccccgtc gagtgaagcg acccgctcgg tggggttgag gccgggcacg
     3841 agaccaccgg ccgcgggatg ccgacgtcca aaggcgtcga gcagcgcggt cacgacggtc
     3901 tttccgcggt cgcccgggcg gcggtctcga gggcccattc gacctgggcc cggatcgtcg
     3961 tctcggccgg tagcgcctgc tcgaacgccg ctccgccgat cgtgaagccc caggctccga
     4021 gatcggtcaa cgcgcggatc tgggcctcgg agacgatcga tccggcgacg acaaccgggc
     4081 cgcgcgaggc gcggacgacg gcgtcgatga tcggctcgac cggcccggtg tgacggtagg
     4141 cgagcaggtc cagcccgtgg acgccgtcgt ggttgctcag gtccgcagcg gatgccgcga
     4201 tctcgtcgat cgtcccgtcg aggaccgatg ggtggccgat gatccgcccc gggaacggga
     4261 agtagcgaag cccggagccg gcaaggagct cgatcgcctc gtcgacgtgg gtgccgccca
     4321 tgacgacgtc gacgccgatg tcgcgggcga cccggatcga gcgcagctcg tcgtccgccg
     4381 agacgctgac gacctcgagg tacacgatcc ggccatcctc gtgcatccgc cgggccagct
     4441 cggcgagcgc ctccggcgac gccccgacgt ccttgaagcc gacatgccgg agctccgtat
     4501 cgcggagcac gtcgtagacg tcgagcgcgt tcgcgacggt ctcgtcgttg tgggtgagca
     4561 tgaagatgaa ctcggccatc ggagggctcc tcaggggtcc gggacgggtg gtctgggtgc
     4621 gagcggcagc ccggcgggct gcgcggcggc tcgagcaagg tcgatcggca cgacgtcggc
     4681 aacgcctgcc agcacgtagc gtggtcgaac gggaccggcg gcaacatcgg cgagggtggt
     4741 ggctccgctc agcacgaggg cggcggccat gcccgcctcg atcgccatcc gcatgtccgt
     4801 cgccaggcgg tcgcccacga gcagggtgtg gtcggccacg agggcaagcc ggtcgaggag
     4861 cgtcgcggcc atgtgggcgc tcggcttgcc cacgatcgcc tcggctcggg cgcccgtgca
     4921 tgcctcgatc gcggccagca tcgcggcaca gtcgggcagg ccgccaccgg gcacggggca
     4981 ccacgcgtcg ggattggtgg cgacgatccg cgcaccagcg cggacggcgt cgaaggcgat
     5041 ctggagcttc gagtaggcga aggtccgatc gaacgagacc acgaccacat cgatccgggc
     5101 agggtcgtca gtgagctccc agcccgcctc ctccaggagg tcccagagca acggctcggc
     5161 gacgggatac agcctggcgc cggacgcgtg gagcccgagg tagcgcagga gtgcatccgt
     5221 cgaggacacg acgtccgcgg atatcgcagg gatccccagc cggctgagct tggcggcgta
     5281 gtcggctggg cgctcgagcg gcttattcgt caggaagacc gtgcggctgc cggccgcacg
     5341 cagggcgagg acggcgtccc tggcacccgg caggagggca tcgccgaggt acagggtccc
     5401 gtcgaggtcg aacacgtagc ccgcgtagcg gcgctcgacg cgggcgagcg ggccgtcccc
     5461 ttccccgtcg ccgatcacag cccggccccg agggcggcgg cgcggtcgag cagccggtcg
     5521 atgtcgggcg gcgtccaggt gctgcatgac gcgcggaggt cgggcaggac gaagcggtcg
     5581 cgcatccgat ggcagttcag gagcgcccag cgggcgaggg ccggcgcgac cggcgggctg
     5641 agctcggcga accgggcggg agcgccagcc cggcggaccg cgccggcgag gacggccggg
     5701 tcgacgacca gcctgcggag cgcggcccga tggcgaggcc agtcggcgag gaaccgctcg
     5761 aacgccggcc gggcgtccga ccagcgcgtg agcttgtgct cgacatcgcg ccagcactcg
     5821 ttggcgaccg cgccgctggg gtcgagcgcg gcgaaggcgg cccggacctc cgcctcgacg
     5881 atcgcggccg ccgggtacgc ggcgtcgacg tcgacggtgg cgggctcgaa cgtgtcgagc
     5941 gtttcggtcc aggcaagtgc gaccacgatc gctgcaacgc cgacctgggc cccgtggaat
     6001 gcgagggagc ggccggcctg ctcggcgtcc atgtcgagga ggtgactcac gatgtgctcc
     6061 gtgccggaga tcggagctgt cttgccggcg acgccgaggg cgatcccgga cagggtcatc
     6121 aggcgggcga ggagggcgag agcgcccggg tcgttggtct ggacggcgtc cgcggcggcg
     6181 agaagcgcct cgccctcgcg ccggaacagg gcgaccggct cggggtgcca gtctgcgtcc
     6241 atcccgagcg caccggccag gtaccaatcg gcgggtgcgg tgaacatcga ggccagctcg
     6301 ccgaaccccg cccggttcat gtcctgtggc gcgtcggcga tgaccgcgag gtcgatcacg
     6361 agggcatccg gccagcgcga cggcatggtc cgcttgacac cgctgcggac gaggacggcc
     6421 atgtcgtcgg agaaggcgtt gaccgagacg gccgtctgga tcgcgacgag cggcacgtca
     6481 ccggcccgat gggtcgcatc cttcgcgatg tcggtgatcg tgccggaccc gacgctgacg
     6541 acgcagccgg cgccctcgat cgcggaatcg gcctcggcca gggcagcgtc gtcggcgtgg
     6601 agctccgccc ggccacggcc gatgacggcg aggcggacct cgccgaccgc ctcgaggagc
     6661 tcgacgacga ggctcttgag gtcgtccggc ccgcgctgca tcggggtcga atcgacgaga
     6721 acgacgaccg ggccggggcg cgccagcgcg cggacgatca cgggcacctc ggccagtgca
     6781 tcctcgccga tccggatcgt tcgcatcccg atcggatgga ggcggcccgc cgggtccgcg
     6841 tcgccgatca tcgtcccgag cgccacgagg tctgtcgggt cgggcatcgg ccgctcggcg
     6901 gccgcggtcg gggacacgtc ccgctcggcg gcgtcggcgc cgatcgaggc cggccgctcg
     6961 tcaggcgaac gatcaggcat ggacgggctc ccggtcgaac agcggctcga tcgcgtcgta
     7021 cagggcgcgg tatgtccgat aggcgtcctc gtaggccggg aacacgcccg gatccggctc
     7081 gtacacccgg tcctcgaggc cgacgagcgc ggtcgcggcc tcgtcgagcg aggcgaacgt
     7141 tccagcgacg accgcgccga tcatcgtcgc cccgagggcg gtcgcctctg gatggacgac
     7201 ccggcgcacg ggcttgccgg tcacgtcggc cttcatctgg aggagcagat cgctgcgcga
     7261 tcctccaccg acgacccgga tctcgccctc cccgaggccg agctcggcaa agcgatcgac
     7321 gatgtcgcgg aggccgaacg tgcagccctc gaacacggcg cgggcgagat ggcccagccc
     7381 atgggccatc gagaggccgt ggaagacggc ccgggcccgc ccgttccagc ggggcgtcat
     7441 cgcacccgac agggccggca cgaagatgac gccgtcggag ccggccggga cgagggcggc
     7501 cgcggcgcag agctcgtcga acgacgaccg accgatcgt
//
LOCUS       DATAIW010000074        21098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_2963, whole genome shotgun sequence.
ACCESSION   DATAIW010000074 DATAIW010000000
VERSION     DATAIW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21098
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHM48_02365"
     CDS             <1..223
                     /locus_tag="VHM48_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2754274.1"
                     /translation="SRTRPYRPQTNGKAERWIRTVLSECLYVEVFHSPGERRLALERF
                     IDYYNGQRPHLGIGGLTPRQRLSELMAAA"
     gene            complement(252..1154)
                     /locus_tag="VHM48_02370"
     CDS             complement(252..1154)
                     /locus_tag="VHM48_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2754275.1"
                     /translation="MVLDGNAVRSVDRAAALLLALGDSQGEAGVTELARRLGLHKSTA
                     SRLLATLQKRGLVEQDEESGKYRLGLVIIRLAERAERTLDLRGIAMPELERLARLTHE
                     TTGLGILDGDTLLTVAQADGPNLIAVGDWTGRSTPLHCVASGKVLLSAVAEREVLRIV
                     RRGLVSYTERTITELEPLLEELARIRRRGYATALGEFEMGLNAVAAPVHDARGNVIAA
                     VDIWGPAFRLTPRRIPELAAQAREAAAAISVRLGGTGTSSAAQPTLAQALSNSSIAAS
                     EPAPLAPSPVASVDELAAEVAAAS"
     gene            complement(1222..2622)
                     /locus_tag="VHM48_02375"
     CDS             complement(1222..2622)
                     /locus_tag="VHM48_02375"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX2754276.1"
                     /translation="MATESRTTSQTPALRRERQLAMYQRALKTLPGGTNSNFRAWGDD
                     TVYIDRGKGGRIWDQDQNEYIDLRMGYGPVILGHGDDRVDDYVNQQMRKGVSFSLTSE
                     DEVRAMELVKELNPWVDMARMTVSGTEATMHAMRLARAFTGRNKIVKFEGQYHGVHDY
                     ALISVAPDNMSDLGDADKPVRLAWGRGIPDAVADTIVPARYNNLAQLRRLFEEQADDI
                     AAVIVEPVMGNAQGILPQAGFHAAMRAMTEEFGILLIFDEVKTGFRLAKGGAAEFFGI
                     TPDIGTYAKAMGNGYPAAAFGGRRDIMSQLPDKVSHGGTYAGNRIAAAAAVKTLEILR
                     DTNALETVHATGRRMQDGLREVLNPSGIPYVFTGHPSMFGIMFTEQVPTEYRDWAKSD
                     HELYDAVALGMLARGAMPEPDSREPWFVCEAHAQGDIVDQVVTIFEESFRAALDSRAR
                     GHVVHVGDETGHASAG"
     gene            complement(3073..4683)
                     /locus_tag="VHM48_02380"
     CDS             complement(3073..4683)
                     /locus_tag="VHM48_02380"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX2754277.1"
                     /translation="MANGYRLGTWPLFTLPADLKWTENPPADANWAFQLHSARYVLDL
                     IAASRITADLAYQDRGLALLQDWIKDNPRHSSPSIWAWNDHSTALRAVVLACTADLTP
                     MTTWLHDSLILHGETLADPGFYRMHGNHALNQNIGLLEVARVLGRADWLTLAGNRMNT
                     LITESIDSEGVTNEQSVGYELYNYKRYRLAENRMLAVGLTPGDGFARLALMPEFLAQA
                     TIPNGYLEMIGDTSGIQKASITGTTAEFAATSGASGPKPTRTIVRFRAGYLFARTGWG
                     EHRPAADETFFSVNWGAGPVFHGHPDGENLTLASFGSRLLVDPGLYSYTSSSYRTFFK
                     SRSANNVVTIDGATWNTAATTRLLGYTATAHYVDVRLGTAGYAGVNQTRHVTYSRGLD
                     YLLVEDRNDSSSVHTYRQLWHLSQDSRPAVGASSVWTQKAKGNLIVRQLAGAPKLDLV
                     KGRTSPVQGWISYSYGTKVAAPVAEAVQTGRHVRYLTLIVPSSGRPTAKASQLVLTST
                     GYSVTITIGVHSERVTVSGSSIWMHTLS"
     gene            5412..6815
                     /locus_tag="VHM48_02385"
     CDS             5412..6815
                     /locus_tag="VHM48_02385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754278.1"
                     /translation="MAHDISSQDPATAISYWLAISPAGLIRPPLDGSATADVAIVGAG
                     FTGLWTALALTDTDPSLQVVVLEQETVGFGASGRNGGFCQASLTHGLANGIRHFPDEL
                     ALLERQGIANLAALVAFTREHGIDCDLEETGGLAVADQPHQVEEFRAWVDEAAEYGEE
                     LVFLDREAIQAEVHSPLWQAGLYQPPGRDVVLDPAKLVRGLARVCEERGIAILEHTRV
                     RAVERRAGGVRIATEGGATVEADHVVVATSAYSGWLRRLETHFVPVYDYVLVSDPMTP
                     EQRASIGWQRRQGMSDANNQFHYFRLTADDRILWGGYDAVYYRNNGVGPQFDRRPATF
                     EKLEGQFRRAFPQLTDLRFPYRWGGAIDTTSRFTVTFGQALGGRLTYAIGYTGLGVGA
                     SRWAAGVVRDFILRPDSDLLRLRFVRSRPFPFPPEPLRWLAVEAVRRELDRADRNEGR
                     RGILLRTLDALGIGFDS"
     gene            complement(6952..8634)
                     /locus_tag="VHM48_02390"
     CDS             complement(6952..8634)
                     /locus_tag="VHM48_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2754279.1"
                     /translation="MPQFDPQPSPVDQLADSFWESLLELNPTTATVYGDERFNDRLED
                     PSPAGRAKTRRLMERTKAAAEAISPDGLPVEDRITRDMLIVIADQAVEQDDQGVHRLG
                     VVDQIGGPQTLLPQVCQFQPADTPERLEKFLARLEAYRDYMAANTDILREGLDTGLTA
                     PRIVAERTIAQLERLMAIPISEAIVPALAQVGSEADRERVRQITADIIYPADRAFLDV
                     LKGDYFKATREEPGLWSATNGESLYRTQIRSWTTLDLDPREVHEIGMEELRIVDIGRR
                     EIAFAAGAASPREYRQRLADDPANTPSSKEELLARAREDIDRAMLVAPRFFGTLPKAG
                     CEVKAVEEFKEKDAPFAYYFPPATDGSRGGTYYANGYDLASRKYTKLATTTYHEAVPG
                     HHFQIALEQENEDLSTFRRLGARIVGGAYVEGWGLYSEKLANEMGLFRNDAERFGMLD
                     AVAWRAARLVVDTGLHALRWTRQQSIDFLLDTGLSETDATIETDRYICWPGQALTYMI
                     GCRAIEKLRRELTARDGSGFDLRGFHDQVLAHGSLPLATLTRELPNWVTTPV"
     gene            complement(8791..11814)
                     /locus_tag="VHM48_02395"
     CDS             complement(8791..11814)
                     /locus_tag="VHM48_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754280.1"
                     /translation="MAGRASLVRAEPGFSLPFFDRLAPPPPPGLVAARIEAHTKPGDV
                     VLDLAGRGGWVGRAAVDRQRRAVSLESTPLTRLLAELVLRPPDLRHLDAAFQAMGASS
                     RGQTSLKVAVGEPFTTRCATCGRTLPLDEVVWPGPAGPDDVTDPAEPYATRSDEPAEV
                     PRRAPIDRDHPAARKFYACPVCRGQRGGEQRSAELDAADLERARTIPDDYVAARARLA
                     ERFPIVDGGTELVDGLLDLHTPRQLVGLEAILERIEGDLRAAPVEAALRLGFLHALLP
                     ASRLNGYPGRIGTLRIQAGRVRPPSAGQWRERHPWLAFEDGIRTVRGFIQRLESGPGG
                     AVQARLGNDLRALDEGTATAVVGISGPSTTRAIGEEASLGRGWDGGPEGVGLRRRIRL
                     VLGQPPVRPNQERLSLTYWATAWTVGAEAAGGLPLAALSGPPIRAPWGWQAASLARLL
                     ASVEPSIARDGQVIELVDGGPEALVAAAMGGVSAGFRLLAARLGDGDDEAFGSVELLP
                     PGAVPPPGPRTRANVALDPLRGGSGDPDIVPGAGLFGAPERFDRRSFSAADVRNTVVE
                     VAVESLKARGEPASADRLLGEVLVGLDRAGQLRRLVADRDRTAPIVGPETETETETET
                     ETEIETDRPAGTADPGPTGLGDPVAGHKHNRSGSTPSPMPGIPPPSAAARVTEGARPS
                     SRPAAPAGAADPPPDRPSIAARRATRLADAPPDQVEALLALIGEGMSGTGRHRLAEVA
                     PGRWWLADPHDRETAAAPLADRVEWAVFSLLSTAGPLSESSFLQRIAGLFTGHDLPDE
                     ALVRACLQSYRSRASTFERTLTGDDLLRRAQEHAELIGLLADGGHRLGLNVWIGRREQ
                     ARRLGAGRLADRLHDAELRAPLSHISRAADEIAEIDCAWYVRGKLAFLFEVEWTAMLG
                     EPILRRHARIPQEEGTVRFLVIAPERTELVRHKLERSPLLREAFDRDNWHVLKWNHLR
                     AFLGRDEPSLDALEPYLGLDPIIERSGEQLGLFGQ"
     gene            complement(12287..13237)
                     /locus_tag="VHM48_02400"
     CDS             complement(12287..13237)
                     /locus_tag="VHM48_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2754281.1"
                     /translation="MLQVLARPIAFRPLAAADPSGSHCIETEIIPVTFPDVETQAPSA
                     VNQTRQKRQLADQAIAQATAAQWSEAAETNRRLLELGPDAESENRLAKAHWELGELGH
                     AREHYQTALALDPTNRIAERNIDRLRILLVDAGEKTVPAQDGSKAAVSIFVEETGKTG
                     FAFLTDLPHPRKLAQVNPGDAVELTPERGRLIAFSNGMRIGVVEPRVAARLLKLMAEG
                     NKYSAGVTSLGDKDVRIIIREIYQDPRNYGKVSFPTAAKSTDLRPYTKGTLIREEMDL
                     EDDLEDDDEDDEIEDLDRVLPPEATTDEAFEEELDEADES"
     gene            13382..15595
                     /locus_tag="VHM48_02405"
     CDS             13382..15595
                     /locus_tag="VHM48_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754282.1"
                     /translation="MRGRRARRIGLGAVIAIAAAAVYVATWTPTDHRTFRPGPGTSGP
                     PSDTPPGVGPIVYYEVLDAEGSRLMERRLDGRSLARQVAERTDVDYGRTWTVDPTGSM
                     AIATIPGPDDQGLEAISIATGASLWSARTPTAPADGAVWSADGQHLALTSIGTDSGPR
                     ETLVLDARTGRFLRLTIPDDAIVQGFELDGTLILRQHIPSPQGVTVGWRFLGVDPATA
                     TIQPLTSLPDVGPASDWSEDVDPAAGLAVDSTLGPNDTGTAIRLWTLRGGSSRTLATV
                     SSADKIVIDPGGTGVAISAAQTIRFVAFDGRAADIFTGPDPIADFSWSVAGDYLAVAT
                     DRRGPNLTIVERATGRSVELPHGDAVAQLLLVRMVGGVPLPPAPLPAVEPTPSPTAGP
                     SGADVAGFGGILSGWVDRSGDTQVAHVERLVPTQVGGLRIAAAMPPLDLGPAAVPDDG
                     GPTLQLLPRPGSNDVLVWIGTSDRSSGWLWDAVDGLRALPLPTDWPDNAFDVAWRPDG
                     LAIAATAGRSTVEGGFEDVFVVAALTGRRTTVVPIVGDYDRLEGWWSPTELRVGHGIC
                     TEGCSGRYAYDARLQIRTHRVVQMTPADRAHGPIDEALVGGGSIVLSMINGDRSDDIV
                     IDWPTELGSRDTLDPLGLAGDGRSLLVARTTATGTDLDRIADPIGRAVGGHVADAQPT
                     LLLHLDGRTLRIDVSPDVQWATVVDRVDNVRLVRLSDGRSWPVDRERVLVWPDGP"
     gene            complement(15663..16577)
                     /locus_tag="VHM48_02410"
     CDS             complement(15663..16577)
                     /locus_tag="VHM48_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754283.1"
                     /translation="MTSSSTNPTRLPARGPARLTRHVPVPNRRPPERGVHGGGRDLVI
                     AATTVVGMAVVLSGPLVWAAAILLLAAAILGTLQVLGDIEGPDVDRGVPVETLFLPAV
                     AAIGCLGAIRLVPFGLAIVPALLLTGLLIDRALVVEARIAAAAQGPTMDDRARALVTM
                     LVVALVAFVGVAAIVPGGIAGLEPAGAPIAPLPLGDLVLLALADAGIAGLLGYRAAAL
                     RATGVREALWAALTYGVAIAIGAAAIRAMGIPRLVGPALLMLLFYLWDSLHATAPARR
                     RDPRWIWETVALVGLGAAVAFWNLRLVG"
     gene            complement(16646..18523)
                     /gene="polX"
                     /locus_tag="VHM48_02415"
     CDS             complement(16646..18523)
                     /gene="polX"
                     /locus_tag="VHM48_02415"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX2754284.1"
                     /translation="MTPARHDLDESPADVLADEAADLTNGDLARVFHEIGDILEVQGE
                     IPFKTIAYHRAADAIGRSPVDLVAAYRSGTEPKVPGVGRAISDKIRELVATGRMAFLD
                     RLRAEVPASLVELLQIPGLGPKTVRQLHEELDIATMAQLREAAESGRIRTLRGLSERT
                     EQLILEGIRRLEAQPPRRMYLHEAEEHVAALVDILGAVPGVTSFESAGSYRRRKETIG
                     DLDLLAETTDPAGLMDRFTSLGTVDHVVARGGHKSAIRLLRGPQVDLMVMPPGSAGAY
                     RLHFTGSKEHNVRLRARARDRGWSLSEYGFQRIGDDGEPLSGDAAELRAFATEQEAYA
                     FLDLPFIEPELREDEGEIEAALAGTLPVLITKADLCGDCHTHSEWSDGSQPIEVMAEA
                     ARRRGYAYQVLTDHSRSLAIANGLTPERVEEERAIIAALNARFAAEEAAGTAPPETPK
                     EGFRLLHGCELEVRADGQLDYDDDLLARFDVVVASVHVARRQSRAELTQRTLNAVGSP
                     HVDIIAHPAGRMIQTRDDLDLDWEAIYAAAARTGTALEVNGSPHRLDLSAERARRAVA
                     AGCILTIDSDAHHTRELDDTRWGVAQARRGWVTAADVLNTRSRDDLLAWVGAKADRIG
                     G"
     gene            complement(18663..20075)
                     /locus_tag="VHM48_02420"
     CDS             complement(18663..20075)
                     /locus_tag="VHM48_02420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2754285.1"
                     /translation="MTPPTERHPSRGTEDPRIRRSWLAFAGAFFLLGLIAVFRLWVDP
                     RLSNWAIALLIVVVGFAAVRHRIAVQDLEAGRRAEAESFARILQGLSRSISPDAIVEA
                     IVEELGIGTGADHIVIVRRRPDARVLEATLVNARTGGSTSSTLFPIADLEDPPDLDVG
                     RWGREPVAIPIDIEGDVGLALSGARPRLGGDAVTVTAGAPRRSGRRMPANAPAADFAA
                     TIAGTRSSRLRDLDPDRLVANRIATRARAVFGLRHTLVTPLTTDGGMIGALLISRRTA
                     GAWPAAAHRLLVGAAIEASAALSRVYSHRQAEARASTDGLTGLPNRRYFDEFCALLSR
                     RRRAEDSVGVLMIDIDKFKVLNDTYGHPVGDEVLRAVAAAIVGAVREDDVPARYGGEE
                     FAVLLRNPTIEVAVEVGERVRAAVAGLDLARFGVRAVRVSVGAAVAEDPDEPITATIE
                     QADRALYDAKRRGRDRVVAA"
     gene            complement(20209..>21098)
                     /locus_tag="VHM48_02425"
     CDS             complement(20209..>21098)
                     /locus_tag="VHM48_02425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX2754286.1"
                     /translation="CAISWWDRPHDRVASIGYYPPVPVAELTPFFDISDYPSTKHVLE
                     DQATVMIDVADPAADPTEVALLVEADNRALVMLPLVASGESIGLVELISAHPIELDDD
                     RLSLSRTIANEASIALENARLYEEARALADRDPLTGFFNHRYLHERFGQEAVRARRNH
                     RPLSLLMLDLDDFKLVNDTFGHLLGDDVLRWTADLIRRTLRGSDVPARYGGDEFAILL
                     PDSDQGEAAATAARLGEAFATSAFSRDGRLTVPIGFSIGAATFPADGRTAAELIAVAD
                     AGLYRMKRSTRRSGESQTA"
BASE COUNT         3000 a         7268 c         7691 g         3139 t
ORIGIN      
        1 cagccggacc cggccgtatc ggccccagac caatggcaag gccgagcgct ggatccggac
       61 ggtcctgtcc gaatgcctct acgtcgaggt cttccactcg cctggcgagc gtcgacttgc
      121 cctcgagcgg ttcatcgact attacaacgg ccaacgtccg catctcggca tcggcgggct
      181 gaccccacgt cagcggctca gcgagctgat ggcggcagct tgagtgtcac caacctcgcg
      241 agggactaca cctaggacgc ggcggcgacc tcggcggcga gctcgtcgac cgaggcgacc
      301 ggcgatggcg ccagcggggc gggctcgctg gcggcgatcg acgagttcga cagggcctgg
      361 gcgagggtcg gctgggcggc agatgatgtg ccggttccgc cgagacggac cgagatcgcc
      421 gctgcggcct cgcgggcctg ggcggccagc tccgggatcc gccgcggggt gagccggaag
      481 gccgggcccc agatgtcgac cgcggcgatc acgttgccgc gcgcgtcgtg gaccggcgcg
      541 gcaaccgcgt tcaggcccat ctcgaactcg ccgagggcgg tcgcgtagcc gcgccgccgg
      601 atccgggcga gctcctcgag gagcggctcg agctcggtga tcgtccgctc ggtgtagctg
      661 acgagaccgc gccggacgat ccggaggacc tcccgctcgg ccacggccga cagcaggacc
      721 ttgccggagg cgacgcaatg aaggggcgtc gaacgaccgg tccagtcgcc gacggcgatc
      781 aggttggggc cgtcggcctg ggcgaccgtc agcagcgtgt cgccgtcgag gatccccagg
      841 ccggtggtct cgtgggtgag ccgggcaagt cgctcgagct ccggcatcgc gatcccgcgc
      901 aggtcgagcg tccgttcggc acgctcggcg aggcggatga tgacgagccc gagccggtac
      961 ttgccgctct cctcgtcctg ctcgacgagg ccgcgcttct ggagcgtcgc gagcagccgc
     1021 gaggccgtgg acttgtggag gccgagccgg cgggcgagct cggtcactcc ggcctcaccc
     1081 tgggaatcgc ccaacgcgag gagaagggcc gccgcccggt ccaccgagcg aaccgcgtta
     1141 ccgtcgagga ccatcgatct gttgtccctc gttgttgtgc cggccgggcc ggtcaggatg
     1201 ccttggtgac gggtgaccgg ttcagccggc cgacgcgtgg ccggtttcgt ctccgacgtg
     1261 gacgacgtgg ccgcgagcgc gtgaatcgag ggccgcccgg aaggattcct cgaagatcgt
     1321 gacgacctgg tcgacgatgt cgccctgggc gtgggcctcg cagacgaacc acggctctcg
     1381 actgtccggt tcgggcatgg ctccccgggc gagcatgccg agcgcgacgg cgtcgtacag
     1441 ctcgtggtcc gacttggccc agtcgcggta ttcggtcggc acctgttcgg tgaacatgat
     1501 cccgaacatc gacggatggc cggtgaagac atacgggatg ccgctcgggt tgaggacctc
     1561 gcgcaggccg tcctgcatcc gtcggccggt ggcatggacg gtctcgagcg cgttcgtgtc
     1621 gcgcaggatc tcgagcgtct tgacggccgc ggcggccgcg atccggttgc cggcgtacgt
     1681 tccgccgtga ctgaccttgt ccggaagctg gctcatgatg tcgcgccgcc cgccgaatgc
     1741 cgcggccggg tagccgttgc ccatcgcctt ggcgtacgtc ccgatgtcgg gcgtgatccc
     1801 gaagaactcg gccgcgccgc ccttggcgag gcggaaaccc gtcttgacct cgtcgaagat
     1861 caggaggatc ccgaactcct cggtcatcgc ccgcatcgcc gcatggaagc ccgcctgcgg
     1921 caggatgccc tgggcgttgc ccatgaccgg ctcgacgatg accgcggcga tgtcgtcggc
     1981 ctgctcctcg aacagccggc gcagctgggc gaggttgttg tagcgggccg gcacgatcgt
     2041 gtcggcgacc gcgtcgggaa tgccccggcc ccaggcgagg cggacgggct tgtcggcgtc
     2101 gccgaggtcg gacatgttgt ccggcgcgac gctgatcagg gcgtagtcat ggaccccgtg
     2161 gtactggccc tcgaacttga cgatcttgtt gcggcccgtg aaggctcggg cgagccgcat
     2221 cgcgtgcatc gtcgcctcgg tccccgacac cgtcatgcgg gccatgtcga cccatgggtt
     2281 gagctccttg acgagctcca tcgcccggac ctcgtcctcg ctcgtgagcg agaagctgac
     2341 gcccttgcgc atctgctggt tgacgtagtc gtcgacccgg tcgtcgccat ggccgaggat
     2401 caccgggccg tagcccatcc gcaggtcgat gtactcgttc tggtcctggt cccagatccg
     2461 cccgcccttg ccacgatcga tgtagaccgt gtcgtcgccc catgcccgga agttcgagtt
     2521 ggtgccaccg ggcagcgtct tcagcgcccg ctggtacatc gcgagctggc gctcgcgccg
     2581 gagggcgggg gtctggctgg tggtcctgga ctcagtggcc atgaggtcac tccgtggggc
     2641 tggttgaccc gtcaagcgcg acgggtgttc cggggagcgg aacggggggc tccatggtgg
     2701 catagggcac gctcgatcgt caacgcggat atcgcacgac tgcaccgatt cacgtgcgaa
     2761 tcacgcacac ttcgccgtga ttcacccctc agaccgcagc aatcggccgc gccggtgagg
     2821 ccgctgatga cggcgccgcc tgccgatggc tggagacttc agtgcgttcg aacgcctgac
     2881 ggcaccaccg ccgacgcgca acgcgcagca gagactcgtc cactcccaca gcctgtcggg
     2941 agacgacaaa gatgcgcgac ctcgaccacg ggtgggcgat cccgtgtgcc cggtgatcgc
     3001 ggctcgactc tgcctcgcct cggcacgcga taccgcgttc aggacgatgc gaggctcagc
     3061 cagattccgg ccctacgaca acgtgtgcat ccagatcgac gaaccactaa ccgttacgcg
     3121 ctcggaatgc accccgatcg tgatcgtgac cgagtagccg gtgctggtca ggaccagttg
     3181 gctcgccttg gcggtcggcc tgccgctcga tgggacgatc aacgtcaggt agcggacgtg
     3241 gcgtccggtc tgcacggcct ccgcgacggg cgctgcgacc ttggtgccgt aggaatacga
     3301 gatccagccc tggaccggag aggtgcggcc cttcacgaga tcgagcttcg gcgctccggc
     3361 aagctgccgg acaatcaggt tccccttggc cttctgggtc cagaccgacg atgctccgac
     3421 cgcgggtcgc gaatcctggg agaggtgcca gagctgtcga tatgtgtgga ccgacgacga
     3481 gtcattgcgg tcttcgacca ggaggtagtc caggccccgt gagtaggtga cgtgccgcgt
     3541 ctggttcacc cctgcgtagc cggccgttcc gaggcgcacg tccacgtaat gggcggtcgc
     3601 ggtgtagccg agtagccgcg tcgtggccgc tgtgttccat gtcgcgccgt cgatcgtgac
     3661 cacgttgttc gccgaccggc tcttgaagaa cgtgcggtag gagctcgagg tgtacgagta
     3721 gagaccgggg tccacgagga gccgggatcc gaatgatgcg agcgtcaggt tctccccgtc
     3781 agggtgaccg tggaagacgg gtccggcccc ccagttcacc gagaagaacg tctcgtcggc
     3841 cgccgggcga tgctcgcccc agcccgtccg cgcaaacaga tacccggccc tgaatcggac
     3901 gatcgtcctg gtcggcttcg gaccggacgc gccgctcgtc gccgcgaact cagcggtcgt
     3961 tccggtaatc gacgccttct ggatcccact ggtgtcgccg atcatctcga ggtagccgtt
     4021 cgggatcgtc gcctgggcca ggaactcggg catcagggcg agccgcgcga agccatcgcc
     4081 gggggtcaat ccgaccgcga gcatccggtt ctcggcgagc cgatagcgct tgtagttgta
     4141 gagctcgtat ccgacggatt gctcgttcgt gacgccctcg ctgtcgatgc tctcggtgat
     4201 cagcgtattc atccggttgc ctgcgagggt tagccagtcg gcccggccca ggacccgggc
     4261 cacctcgagg aggccgatgt tctggttcag ggcgtgattg ccgtgcatcc ggtagaagcc
     4321 cggatcagcc agcgtctcgc cgtgcagaat caggctgtca tggagccagg tggtcatcgg
     4381 cgtgaggtcg gcggtgcaag cgagcacgac cgcccgaagc gcggtcgagt gatcgttcca
     4441 ggcccagatc gatggtgacg agtggcgcgg attgtccttg atccagtcct ggagcagcgc
     4501 cagtccgcgg tcctggtatg ccaggtcggc cgtgatccgg gacgccgcaa tcaagtcgag
     4561 aacgtagcgg gcggagtgca gttggaacgc ccagttggcg tccgcgggcg ggttctcggt
     4621 ccatttcagg tcggcgggca gcgtgaagag cggccacgtt cccagtcgat acccgtttgc
     4681 catgagctcg tttgcggtcg cgaccgggtc tgacgagttg cggcagtcct ggctgagcgg
     4741 gacatcggag gtggtgacta atgcacgcgc gtgcggccca gtccgacagc ggcacgccgt
     4801 cgaggattgg tgcgacggtt ggcgagggtt tcggggatgg ccccgtgtca gagcctccgt
     4861 cggcggccga gccggtgatc ccgaagctga gaagggccag acttgcggcg aagagggttg
     4921 cccgccatcg agcgcgggtt gccatccgtc ggcgcggccg cgtgcccccc aaagccatcg
     4981 ttgacctcta ccttcgaata tcgcgtgttg gctggaaagc cccacaacct ccggagccag
     5041 gccagctgac gcgcaagcgt ggttgacacg tgatccaagt gtaatggccc ggatcatgcc
     5101 gcgaccagga caccagatgt cccactccaa tcctggaccc gttcggcggc gtcccatcgg
     5161 accgacgggc cccggacgac cgacgcgggc aacctgacgg cggcgcgtcg cccgagctcg
     5221 cgctgcgacc agccgacggt tttccttgcc tcgcgaatca cgcgcccggc gtgggaggcg
     5281 acgatcgttc cggtcctcgc gtcgtccgtt cacccaccaa agccctgtag cagtcgcaac
     5341 ttgcgccggg ctcatcgggc gaggtgcgcg acggaggccg tcgcgagcag ccgccgcgct
     5401 acgatccggc catggcccac gacatatcct cgcaggatcc cgccacggcg atcagctact
     5461 ggctcgccat ctcgcctgcg ggcctgatcc ggccgcccct ggatggctca gcgaccgccg
     5521 acgtcgcgat cgtcggtgcc gggttcaccg ggttgtggac cgcgctcgcg ctgaccgaca
     5581 cggacccgtc gctccaggtg gtggttctcg agcaggagac cgttggattc ggggcgagcg
     5641 ggcggaacgg ggggttctgc caggcgtcgc tcacccacgg cctcgccaac gggatccgcc
     5701 atttccccga cgagctggcg ctgctcgagc gccaaggaat cgccaacctc gcagcactgg
     5761 tcgccttcac ccgcgagcac ggcatcgact gcgacctcga ggagacgggt gggctcgcgg
     5821 tcgccgacca gccccaccag gtcgaggagt tccgggcctg ggtcgacgaa gcggccgaat
     5881 acggcgaaga gctcgtgttc ctcgaccgcg aggcgatcca ggcggaggtc cattcgccgc
     5941 tctggcaggc gggcctgtac cagccgcccg gccgcgatgt ggtcctcgat ccggccaagc
     6001 tggtccgcgg cctcgcccgc gtgtgcgagg agcgcgggat cgcgatcctc gagcacaccc
     6061 gggtccgcgc ggtcgagcgg cgggccggcg gcgtccggat cgcgacggag ggaggcgcga
     6121 ccgtcgaggc cgatcacgtg gtcgtcgcca cgtcggccta ctccggctgg ttgcgccgtc
     6181 tcgagactca tttcgttccc gtctacgact acgtcctcgt gtcagatccg atgacgcccg
     6241 agcagcgcgc ctcgatcggc tggcagcggc ggcagggcat gtccgacgcg aacaaccagt
     6301 tccactactt ccgcctcacg gccgacgacc ggatcctgtg gggcggctac gacgcggtct
     6361 actaccgcaa caacggcgtc gggccgcagt tcgaccgacg accggcgacc ttcgagaagc
     6421 tggaggggca gttccggcgc gcatttccgc agctgaccga cctccgtttc ccctaccgct
     6481 ggggcggggc gatcgacacg acctcgcggt tcacggtcac cttcggccag gctctcggcg
     6541 gacggctgac ctacgcgatc ggctacaccg ggctcggcgt cggggcgagc cgctgggcgg
     6601 cgggcgtcgt ccgcgacttc atcctccgtc cggactccga cctgctccgg ctgcggttcg
     6661 tccggagcag gccgttcccg ttcccgcccg agccactccg ctggctcgcg gtcgaggccg
     6721 tccgccgcga actcgaccgg gccgatcgga acgagggccg ccgggggatc ctgctccgaa
     6781 ccctcgacgc cctcgggatc ggtttcgaca gctagggtcc gcggccggtc gcgctgcccg
     6841 gttcgggcgt ggggcgcccg aacgggcgga cgaaccgggc ccgggcggcg ctggttcgct
     6901 ggttcggggg cagggcgccc gaacgggcgg aataggagcg ccctcggccg gtcagacggg
     6961 cgtcgtgacc cagttcggaa gctcgcgggt cagcgtcgcc aggggcagcg agccgtgggc
     7021 gaggacctgg tcgtggaagc cgcgaagatc gaaccccgag ccgtcgcggg ccgtcagctc
     7081 gcggcggagc ttctcgatcg cgcggcagcc gatcatgtac gtcagcgcct ggcccggcca
     7141 gcagatgtag cggtcggtct cgatcgtcgc gtcggtctct gacaggcccg tgtcgagcag
     7201 gaagtcgatc gactgctggc gcgtccagcg aagggcatgc agaccggtgt cgaccaccag
     7261 gcgggccgct cgccaggcga ccgcgtcgag catgccgaag cgctcggcgt cgttgcggaa
     7321 caggcccatc tcgttggcaa gcttctcgct gtagaggccc cagccctcga cataggcgcc
     7381 gccgacgatc ctggccccga gcctgcggaa ggtcgagagg tcctcgttct cctgctcgag
     7441 ggcgatctgg aagtgatggc cggggacggc ctcgtggtag gtcgtcgtcg cgagcttcgt
     7501 gtacttccgg ctcgccaggt cgtagccgtt ggcgtagtag gtgccgccgc gcgagccgtc
     7561 ggtggccggc gggaagtagt aggcgaaggg cgcgtccttc tccttgaact cctcgaccgc
     7621 cttgacctcg cagccggcct tcggcaacgt cccgaagaag cgcggggcga cgagcatcgc
     7681 tcggtcgata tcctctcggg cacgggcgag aagctcttcc ttggacgacg gggtattcgc
     7741 cgggtcgtcg gccagccgct ggcggtattc ccgcgggctg gccgcgccgg ccgcgaacgc
     7801 gatctcgcgc cggccgatgt cgacgatccg gagctcctcc atcccgatct cgtggacttc
     7861 gcgcggatcg aggtcgaggg tcgtccagga ccggatctgc gtccggtaca gcgattcacc
     7921 gttcgtggct gaccacaggc ccggctcctc gcgggtggcc ttgaagtagt cacccttgag
     7981 gacgtcgagg aatgcccggt cggccgggta gatgatgtcc gcggtgatct gccggacccg
     8041 ctcgcggtcg gcctccgaac cgacctgggc gagcgcgggg acgatcgcct ccgagatcgg
     8101 gatcgccatc aggcgctcga gctgggcgat cgtccgctcg gcgacgatgc gcggcgcggt
     8161 caggccggta tcgaggccct cgcggaggat gtccgtgttc gcggccatgt agtcgcggta
     8221 ggcctcgagg cgagcgagga acttctcgag ccgctcgggc gtgtcggccg gctggaactg
     8281 gcagacctgt ggcaggagcg tctgggggcc gccgatctgg tcgacgacgc cgaggcggtg
     8341 gacgccctga tcgtcctgct cgaccgcctg gtcggcgatg acgatcaaca tgtcgcgggt
     8401 gatccgatcc tcgaccggga gcccgtctgg cgagatggcc tcggccgcgg ccttggtccg
     8461 ctccatcagc cggcgcgtct tcgcccggcc ggccgggctg ggatcctcga gacgatcgtt
     8521 gaaccgctcg tcgccataga cggtcgcggt cgtcggattg agctcgagga ggctctccca
     8581 gaagctgtcg gcaagctgat cgacgggcga gggctgagga tcgaattggg gcacgttgtc
     8641 tccaggttgg gcgggggacg gatgcgtctc gaccccgagg acagtggtga gcagagcgta
     8701 gcgcgcgggg ccggatcggg cccgggcggc atcgggcccg gcggcaacgg gccgggcgag
     8761 cgcggtccgc cggcgttcgc gtccgcgggc ctactgcccg aacaggccca gttgctcgcc
     8821 gctccgctcg atgatcgggt cgaggccgag atacggctcc aaggcgtcga gcgacggctc
     8881 gtcacggcca aggaacgcgc ggagatggtt ccacttgagg acgtgccagt tgtcgcggtc
     8941 gaaggcctcc cgcaggagcg gcgagcgctc gagcttgtgg cggacgagct cggtccgctc
     9001 cggggcgatg acgaggaacc ggaccgttcc ctcctcctgc gggatccggg cgtggcggcg
     9061 gagaatcggc tcaccgagca tcgccgtcca ctcgacctcg aacaggaagg cgagcttgcc
     9121 ccggacgtac cacgcgcagt cgatctcggc gatctcgtcg gcggcccggc tgatgtgcga
     9181 aaggggagcc cggagctcgg catcgtggag ccgatcggcg agccgtccgg cgccgagccg
     9241 gcgcgcctgc tcgcgccgcc cgatccagac attcagcccg agccggtgcc cgccgtcggc
     9301 gaggagcccg atcagctcgg cgtgctcctg ggcgcgccgc aggaggtcgt cgccggtcag
     9361 ggtccgctcg aaggtgctgg cccggctgcg atagctctgg aggcaggccc gcacgagcgc
     9421 ctcgtcgggc aggtcgtggc cggtgaacag gccggcgatc cgttggagga aggaggattc
     9481 cgacagcggc cccgcggtcg agaggaggct gaagaccgcc cactcgaccc ggtcggcgag
     9541 cggcgccgca gccgtctcgc ggtcatgcgg atctgccagc caccagcggc cgggggcgac
     9601 ctccgccagg cggtggcgac cggtgccgga catcccttcg ccgatcaggg cgagcagtgc
     9661 ctcgacctgg tcgggcggcg catcggcgag ccgggtcgcg cgccgggccg caatcgacgg
     9721 tcgatccggc ggcgggtcgg ccgcacctgc cggcgccgca ggtcgcgagc tcggtcgggc
     9781 gccctcggtg acccgcgcgg cggcactcgg cggcgggatc ccgggcatgg gtgagggggt
     9841 cgagccggat cggttgtgct tgtgaccggc gaccggatcg ccgagtcccg tcgggcccgg
     9901 gtcggcggtg cccgctggcc ggtcggtctc gatctcggtc tcggtctcgg tctcggtctc
     9961 ggtctcggtc tcggggccga cgatcggcgc cgtccggtcg cgatccgcga cgaggcgtcg
    10021 gagttggccc gcccgatcca ggccgacgag gacctcgccg agcaggcggt cggccgatgc
    10081 cggctcgcca cgcgccttca ggctctcgac cgcgacctcg acgacggtgt tgcggacgtc
    10141 ggcggccgag aacgaccgac ggtcgaagcg ttccggagcg ccgaacaggc ccgcgccggg
    10201 gacgatgtcg ggatcgccgg agccgccacg caggggatcc agcgcgacgt tcgcccgagt
    10261 ccgtggaccg ggtggcggga ccgcgcccgg cggcaggagc tcgacgctgc cgaaggcctc
    10321 gtcgtcgccg tcgccgagtc tggccgcgag caaccggaac ccggcgctga cgccgcccat
    10381 cgccgcggcg acgagcgcct ccggaccgcc gtccacgagc tcgatgacct ggccgtcccg
    10441 cgcgatcgac ggctccaccg aggcgagcag acgagcgagc gacgccgcct gccaacccca
    10501 cggcgcccgg atcggaggac cggacagggc cgcgagcggg agtccgccgg ccgcctccgc
    10561 gccgaccgtc catgccgttg cccagtaggt cagcgagagc cgctcctggt tgggccgaac
    10621 cggtggctgg ccgaggacca gccggattcg acgacgaagg ccgacgccct cgggcccgcc
    10681 gtcccacccg cgcccgagtg acgcctcctc cccgatcgcc cgtgtcgtcg atggcccgct
    10741 gatcccgacg acggcggtag cggtgccctc gtcgagcgcg cggagatcgt tgccgagacg
    10801 tgcttgaacg gcgcccccgg gcccgctctc gagccgctgg atgaagccgc gaaccgtccg
    10861 gatgccgtcc tcgaacgcga gccacgggtg acgctcgcgc cattgcccgg ccgaaggggg
    10921 acggacccga ccggcctgga tccggagcgt cccgatcctg cccggatagc cgttgaggcg
    10981 gctcgccggc agaagggcgt ggaggaagcc gaggcgaagg gccgcctcga ccggcgcggc
    11041 tcggaggtcg ccctcgatcc gctcgaggat cgcctcgagc ccgacgagct gacgcggcgt
    11101 gtggaggtcg agcaggccgt cgacgagctc ggtaccgccg tcgacgatcg ggaaccgctc
    11161 ggcgagccgt gcgcgggccg ccacgtagtc gtccgggatc gtccgggccc gttcgaggtc
    11221 cgctgcgtcg agctcggcgc tgcgctgctc cccaccccgt tggccgcggc acaccgggca
    11281 ggcgtagaac ttgcgggcgg ccggatggtc gcggtcgatc ggcgctcgtc gcgggacctc
    11341 cgcgggctcg tcggaccgcg tcgcatacgg ctcggccggg tcggtcacgt cgtccgggcc
    11401 ggcgggcccg ggccacacca cctcgtcgag cggcagcgtc ctgccgcatg tcgcacagcg
    11461 agtcgtgaat ggctcgccga ccgccacctt caggctggtc tggccgcgcg acgatgcgcc
    11521 catggcctgg aacgcggcat caaggtggcg caggtccggc gggcgcagga cgagctcggc
    11581 gaggagtcgc gtcagcggcg tggattcgag gctgacggcc cggcgctggc gatcgacggc
    11641 tgcgcggccg acccagccgc cccgaccggc aagatcgagg acgacatcgc cgggcttggt
    11701 gtgagcctcg atccgggcgg ccacgagacc cggcggaggc ggcggcgcga gccgatcgaa
    11761 gaagggcagc gagaagccgg gctccgctcg aaccagggac gcgcgaccgg ccatgcctac
    11821 agcctagccc ggcacgtcca cgccgtcgcg gtcgggcggg cgccatggtc ccgactcagc
    11881 caccgagtca agacgatggg cggcgcgctc ggccgatccc gaatcggcac gcggccacgc
    11941 tcgcggttcg tcgaattgac tcaactgctg agtcagcgcg atgccgccct cgcgttggcg
    12001 tcgacgctgg ggtgacgcga ctgcgttcgt cgcgctgggg tgaggctggc aaggcgccgt
    12061 gactgcgttc gtcgcggatg ggtgaggctg gcaaggcgcc gtgactgcgt tcgtcgcgga
    12121 tgagtccgtc agcgcaaggc gcgcccgagc accggagcga gcggcctccg cgcccggagg
    12181 tccgaggcgg agctccgcga cgccatcggc ccgacggaat ggcttgggcg agggagcgca
    12241 gggcgcaacg acgcgatggc gtgctcagcc gcgacagccg gtgatgctag ctctcatctg
    12301 cctcgtcgag ctcttcctcg aaggcttcgt cggtcgtggc ctcgggcggc aggacccgat
    12361 cgagatcctc gatctcgtcg tcctcgtcgt cgtcctcgag atcgtcctcg aggtccatct
    12421 cctcgcggat cagggtgccc ttcgtgtatg gccgcaggtc ggtcgacttg gccgcggtcg
    12481 ggaaactgac cttgccatag ttgcgcgggt cctggtagat ctcgcggatg atgatccgga
    12541 cgtccttgtc gccgagcgac gtgacgccgg ccgagtactt gttgccctcg gccatgagct
    12601 tcaggaggcg ggccgcgacc cgcggctcga cgaccccgat ccgcatcccg ttgctgaagg
    12661 cgatcaaccg accgcgctcc ggggtcagct ccaccgcatc gcccggattg acctgggcga
    12721 gcttgcgcgg atggggcagg tcggtcagga atgcgaagcc ggtcttgccg gtctcctcga
    12781 cgaagatcga gaccgcggcc ttgctgccgt cctgggccgg aaccgtcttc tcgccggcgt
    12841 cgacgagcag gatccgcagc cggtcgatgt tccgttcggc gatccggttc gtcggatcga
    12901 gggccagcgc ggtctggtag tgctcgcggg catgaccgag ctcgccgagc tcccaatgcg
    12961 ccttcgccag gcggttctcc gattccgcat cgggcccgag ctcgagcaga cggcggttcg
    13021 tctcggccgc ctctgaccac tgggccgccg tcgcctgggc gatcgcctgg tccgccagct
    13081 ggcgcttctg ccgcgtctgg ttgacggcgc tcggtgcctg ggtctcgacg tcgggaaagg
    13141 tcacgggaat gatctccgtt tcgatgcagt gcgaacccga tgggtcggcc gcggcgaggg
    13201 gccggaacgc gatcgggcgg gcaaggactt gtagcatcgc gggactcgca cgtcaaaccc
    13261 ggccccgggt cacgagggcc tcgcggccga gatgccggtc gtgtcggccg gttccgcaac
    13321 aggccgggct cggccgacgt ctcgtcgccg tcgagcagcg aagatggtga tgatgggatc
    13381 gatgcgggga cggcgggcgc gccggatcgg actgggggcg gtgatcgcga tcgccgcggc
    13441 agccgtctac gtcgcgacgt ggaccccgac ggaccaccgg accttccggc cggggccggg
    13501 gaccagtggc ccgccatcgg acaccccgcc cggggtcgga ccgatcgtct actacgaggt
    13561 cctcgatgcc gaggggtcac gcctgatgga gcggcgcctg gacggtcgct cgctggctcg
    13621 ccaggtcgcc gaacgaaccg acgtcgacta cggccggacg tggaccgtcg acccgaccgg
    13681 cagcatggcg atcgcgacga tccccggtcc cgacgaccag ggactcgagg cgatctcgat
    13741 cgcgaccggc gcctcgctct ggtcggcccg gacgccgacc gctccagccg atggggccgt
    13801 ctggtcggcg gacgggcaac acctcgcgct cacctcgatc ggcactgaca gcggccctcg
    13861 ggagaccctc gtgctcgacg cgcggaccgg tcgattcctg cggctcacga tccccgacga
    13921 tgccatcgtc cagggcttcg agctcgacgg cacgctcatc cttcgccagc acatcccttc
    13981 gccacagggg gtgactgtcg gatggcggtt cctgggggtc gacccggcca cggccacgat
    14041 ccagcccctg accagcctgc ccgacgtcgg gccggcgagc gactggtccg aggacgtcga
    14101 cccggccgcc ggcctggccg tcgactcgac gctcggtccg aacgacacgg gcaccgcgat
    14161 ccggctgtgg acgctccgcg gcggctcctc gcgaaccctc gcgaccgttt cctcggccga
    14221 caagatcgtg atcgatccgg gcggaaccgg cgtcgcgatc agcgccgccc agacgatccg
    14281 gttcgtggcg ttcgatgggc gcgcggcgga catcttcacc ggtcccgacc cgatcgcgga
    14341 cttcagctgg tcggtggccg gcgactacct ggccgtcgcg accgatcgtc gcggaccgaa
    14401 cctcacgatc gtcgagcggg cgacgggacg ctccgtcgag ctgccccacg gggacgcggt
    14461 cgcccagctc ctcctcgtcc ggatggtcgg cggggtgccg ctgccgccgg cgcccctgcc
    14521 cgccgtggag ccgacaccgt cgccgaccgc cggaccgtcc ggtgccgatg tcgcggggtt
    14581 cggcggcatc ctgtcggggt gggtcgatcg gagtggtgac acccaggtcg cccacgtcga
    14641 gcggctcgtc ccgacccagg tcggtgggct ccgcatcgcg gcggccatgc cgcccctcga
    14701 tctcggaccg gccgcggttc ccgacgacgg cggcccgacg ctgcagctcc tcccccggcc
    14761 cggctcgaac gacgtcctcg tctggatcgg gacgtccgat cgatcgtcgg gctggctctg
    14821 ggacgcggtg gatggactgc gggccctccc gctgccgacc gactggccgg acaacgcctt
    14881 cgatgtcgcg tggcggcccg atggcctcgc gatcgccgcg acagccgggc gatcgacggt
    14941 cgagggcggg ttcgaagacg tcttcgtcgt cgccgccttg accggtcgga ggacgacggt
    15001 cgtgccgatc gtcggcgact acgaccggct cgagggctgg tggtcgccga ccgagttgcg
    15061 ggtcgggcac ggcatctgca ccgagggctg ctcgggccgc tatgcctacg atgcgcggct
    15121 ccagatccgg acccatcgcg tcgtccagat gacccccgcc gaccgggccc acggaccgat
    15181 tgacgaggcc ctcgtcggtg gcggctcgat cgtgctgtcc atgatcaacg gggaccgatc
    15241 cgacgacatc gtcatcgact ggccgaccga gctcggttca cgcgacacgc tcgacccgct
    15301 cggcctcgct ggcgacggac gatcgcttct cgtggcccgg acgacggcga ccggcaccga
    15361 tctcgatcgc atcgccgatc cgatcggccg tgcggtcggc ggccacgtcg ccgacgcaca
    15421 accgaccctg ctccttcacc tggatggccg gaccctgcgg atcgacgtgt cacctgacgt
    15481 tcaatgggcg acggtcgtcg atcgggtcga caacgtccgc ctcgtccgcc tgtccgacgg
    15541 tcggagctgg ccggtcgatc gcgagcgggt cctcgtctgg ccggacggac cctgaggctc
    15601 gagccgacgc ggcatcgagg ccggccccat tggatcgaac gcgccgtgag gcgggcgaat
    15661 gatcagccga cgaggcgcag gttccagaac gcgacggcgg caccgaggcc gacgagggcg
    15721 accgtctccc agatccagcg cgggtcgcgc cgccgtgccg gcgcggtcgc atggagcgaa
    15781 tcccagaggt agaagagcag catcaggagc gccggcccga cgagccgcgg gatgcccatc
    15841 gcccggatcg ccgccgcacc gatggcgatc gccacgccgt acgtcagggc cgcccacagc
    15901 gcctcgcgga cgcccgtcgc gcggagggca gccgcgcgat atccgagcag gcccgcgatg
    15961 ccggcgtcgg cgagggccag cagcacgagg tcgccgagcg gcagcggcgc gatgggcgct
    16021 ccggcgggct cgaggccggc gatcccgccg ggaacgatcg ctgccacgcc gacgaaggca
    16081 acaagagcca cgaccagcat cgtgaccaga gctcgggcgc ggtcgtccat ggtcgggccc
    16141 tgcgcggcgg cggcgatccg ggcctcgacg acgagcgccc ggtcgatcag caggcccgtc
    16201 agcaggaggg ccgggacgat cgcgaggccg aacgggacga gacggatcgc cccaaggcac
    16261 ccgatcgccg cgacggccgg caggaagagc gtctcgaccg gcacgccgcg atccacgtcc
    16321 ggtccctcga tgtcgccgag gacctggagg gtcccgagga tcgccgcggc gaggaggagg
    16381 atcgcggccg cccagacgag cggcccgctc aggaccacgg ccatcccgac gaccgtcgtt
    16441 gccgcgatca ccaggtcgcg accgccgccg tgcaccccac gttcgggtgg tcgtcgattc
    16501 ggcaccggga cgtggcgcgt cagtcgagcc ggaccacggg ccgggagccg ggtcgggttc
    16561 gtcgacgagc tcgtcatcgg ccggatggta cgcgtttcgc tcacgggtcc gggccgtcga
    16621 gcgcggaccg accgatcgcg accggtcaac cgccgattcg atccgccttc gccccgaccc
    16681 acgcgagcag gtcgtcccgg gaccgcgtgt tcagcacgtc ggccgcggtg acccagccac
    16741 gccgagcctg agccaccccc cagcgcgtgt cgtcgagctc gcgggtgtgg tgggcgtcgg
    16801 agtcgatcgt caggatgcag cccgcggcga ccgcccgacg ggcccgctcg gcggacaggt
    16861 cgagccgatg gggcgaaccg ttgacctcga gcgctgtccc ggtccgagcc gccgccgcgt
    16921 agatcgcctc ccaatccagg tcgaggtcgt cgcgggtctg gatcatccgc cccgcggggt
    16981 gagcgatgat gtcgacgtgg gggctgccga cggcgttgag cgtccgctgc gtcagctccg
    17041 cccgcgactg gcgacgggcg acgtggaccg acgcgacgac gacgtcgaac cgggccagca
    17101 gatcgtcgtc gtagtcgagc tggccgtcgg cccggacctc cagctcacaa ccgtggagga
    17161 ggcgaaaccc ctccttcggc gtctcgggcg gcgccgtccc ggcggcctcc tcggcagcga
    17221 accgggcgtt gagcgcggcg atgatcgccc gctcctcctc gacgcgctcc ggcgtcagcc
    17281 cgttggcgat cgcgagcgag cggctgtggt cggtcaggac ctggtacgcg tagcctcgac
    17341 ggcgggcagc ctcggccatg acctcgatcg gctggctgcc gtcgctccac tcgctgtggg
    17401 tgtggcagtc accgcaaagg tcggctttcg tgatcaacac cggcagggtc ccggcgagtg
    17461 ccgcctcgat ctcgccctcg tcctcgcgca gctcgggctc gatgaacggc aggtcgagga
    17521 acgcgtaggc ctcctgttcg gttgcgaagg cccggagctc ggcggcgtcg ccggatagcg
    17581 gctcgccatc gtcgccgatc cgctggaagc cgtactcgga caggctccag ccgcgatcgc
    17641 gggcccgcgc ccggagccgg acgttgtgct ccttggaccc ggtgaagtga aggcggtagg
    17701 cgccggcgct ccctggcggc atcaccatca ggtcgacctg cggcccgcgc agcaggcgga
    17761 tcgccgactt gtggccaccc cgtgcgacga catggtcgac cgtcccgagg ctcgtgaacc
    17821 ggtccatgag ccccgccgga tcggtcgtct cggcgagcag gtcgagatcg ccgatcgtct
    17881 ccttgcggcg acgatacgat cccgcggact cgaatgacgt gacgcccggc acggcgccga
    17941 ggatgtcgac cagcgcggcg acgtgctctt cggcttcatg gaggtacatc cgccggggcg
    18001 gctgcgcctc gaggcgccgg atcccctcga ggatcagctg ttccgtccgc tccgacaggc
    18061 cgcgcaacgt gcggatccga ccgctctcgg cggcctcccg gagctgggcc atcgtcgcga
    18121 tgtcgagctc ctcgtggagc tggcggacgg tcttcgggcc gagccccggg atctggagca
    18181 gctcgacgag gctggccggg acctcggcac ggagccggtc gaggaacgcc attcggccgg
    18241 tcgcgacgag ctcacggatc ttgtcgctga tcgctcggcc gacgccgggg accttgggct
    18301 ccgtccccga gcggtaggct gccacgaggt cgaccgggct gcgcccgatc gcgtccgcgg
    18361 cgcggtgata agcgatcgtc ttgaacggga tctcgccctg gacctcgagg atgtcgccga
    18421 tctcgtggaa gacgcgggcc agatcgccgt ttgtcaggtc ggcggcctca tcggcgagga
    18481 catcggccgg cgactcatcc aggtcgtggc gggcaggcgt caccggcgcg gaatcgactg
    18541 tcgacgtccg aggcgatcgg tcgcgtggca tcgcgggcga tggtagcgcg gagtcgatgt
    18601 cgggacggcc tgagggcgcg gggaactcgg agcgcgaggc ggagcaacct caggcggctc
    18661 cgttacgcgg cgacgacccg gtcacggcct cggcgcttcg cgtcgtacag cgcgcgatcg
    18721 gcctgctcga tcgtcgcggt gatcggttcg tcgggatcct cggcgacggc ggccccgacc
    18781 gagaccctca ccgcgcgcac cccgaaccgg gcgagatcga gcccggccac ggccgcgcgg
    18841 acgcgctcgc cgacctcgac ggccacctcg atcgtcgggt tgcgcagcag gaccgcgaat
    18901 tcctcgccgc cgtagcgggc gggcacgtcg tcctcgcgaa ccgcgccgac gatcgccgcg
    18961 gccaccgccc gcaggacctc gtcgccgacc gggtggccgt acgtgtcgtt gaggaccttg
    19021 aacttgtcga tgtcgatcat caggaccccg acgctgtctt cggcccggcg gcggcgggac
    19081 aggagggcgc agaactcgtc gaaatagcgc cgattcggga ggccggtcag gccgtcggtc
    19141 gaggcccgcg cctccgcctg gcggtgcgag tagacgcgtg acagagcggc ggaggcctcg
    19201 atcgcggcgc cgaccagcag ccgatgggcg gcggccggcc aggcgcccgc ggtccgccgc
    19261 gagatgagca gcgccccgat catcccgcca tcggtcgtga gcggagtgac gagcgtgtgg
    19321 cgcaggccga agacggcccg ggcgcgcgtg gcgatccggt tggcgaccag ccgatcggga
    19381 tcgaggtcgc gcagccggct ggatcgagtc ccggcgatcg tcgccgcgaa atcggcggcc
    19441 ggcgcgttcg ccggcatccg ccgaccactt cgtcgtgggg cgccggccgt gaccgtgacc
    19501 gcgtcgccgc cgaggcgtgg tcgggctccg gagagcgcga ggccaacatc gccttcgatg
    19561 tcgatcggga tggcgaccgg ctcgcgaccc cagcggccaa catcgaggtc cggcgggtcc
    19621 tcgagatcgg cgatcgggaa gagcgtcgac gatgtcgatc cgccggtccg ggcgttgacc
    19681 agggtcgcct cgaggacgcg ggcgtccggg cggcgccgga cgatgacgat gtggtcggca
    19741 ccggtcccga tcccgagctc ctcgacgatc gcctcgacga tcgcatcggg cgagatcgag
    19801 cgggacagac cctggaggat cctggcgaag ctctcggcct cggcacggcg gcccgcctcg
    19861 agatcctgga cggcgatccg gtgacggacc gcggcgaacc cgacgacgac gatcagcagg
    19921 gcgatggccc agttgctgag ccgcgggtcg acccacagcc ggaagaccgc gatcaggccg
    19981 aggaggaaga acgctccggc gaaggcgagc caggaccggc ggatccgtgg atcctccgtc
    20041 cctcgggatg gatggcgttc ggtcggtggg gtcacgttcg gtgatgagca tgacggtccg
    20101 gtgcgcttcg ccgcaagtcg aacgggcagt acttccggcg ggccggcccc ggtaccttcg
    20161 gcgaccggct cgccgaggag atgggtcggg acgctccgaa ccgcgtgatc aggccgtctg
    20221 cgactcccct gaccgacgcg tcgagcgctt catccggtac aggcccgcat cagccacggc
    20281 gatgagctcg gccgcggtcc ggccgtcggc gggaaacgtc gcggcgccga tcgagaagcc
    20341 gatcgggacc gtcagccgtc cgtcgcggga gaaggccgag gtcgcgaatg cctcgccgag
    20401 gcgggccgcc gtcgccgcgg cctcaccctg gtcggaatcc ggcagaagga tcgcgaactc
    20461 gtcgccgccg taccgcgccg gcacgtccga gccgcggagg gtcctccgga tgagatcggc
    20521 ggtccagcgc aggacgtcgt cgccgaggag gtgtccgaag gtgtcgttga cgagcttgaa
    20581 gtcgtcaagg tcgagcatca ggaggctcaa cgggcgatgg ttgcgacggg cccggaccgc
    20641 ctcctggccg aaccgctcgt ggagatagcg gtggttgaag aacccggtca gggggtcgcg
    20701 gtccgcgagc gcgcgcgcct cctcgtacag gcgggcgttc tcgagggcga tcgaggcctc
    20761 gttggcgatc gtccgcgaca ggctcaaacg gtcgtcgtcg agctcgatcg ggtgggccga
    20821 gatcagctcg acaaggccga tcgactcgcc ggacgcgacg agcggaagca tgacgagcgc
    20881 ccggttgtcg gcctcgacga ggagggcaac ctccgtcggg tcggcggccg ggtcggcgac
    20941 gtcgatcatg acggtcgcct ggtcctcgag cacgtgcttg gtcgacgggt agtccgagat
    21001 gtcgaagaac ggcgtgagct ccgcgaccgg cacgggcggg tagtagccga tgctggcgac
    21061 tcggtcgtgg ggccggtccc accacgagat cgcgcatt
//
LOCUS       DATAIW010000075        21730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_152171, whole genome shotgun sequence.
ACCESSION   DATAIW010000075 DATAIW010000000
VERSION     DATAIW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21730
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152171"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..897)
                     /gene="folK"
                     /locus_tag="VHM48_02430"
     CDS             complement(277..897)
                     /gene="folK"
                     /locus_tag="VHM48_02430"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2754287.1"
                     /translation="MRRVRAYIGLGANLGDPARTLAGGVRALAALSGARLHAVSRLYM
                     TEPVGVVDQPSFRNAVVAIDVPAGPDPATGATALLIALKQLERAFGRREGERWGPREL
                     DLDLLIFGRARLSLDRPPEGLSLDPGKADRLLVVPHRDTAQRLFVLAPLADVAPGLVP
                     PGWPETVATAAARRLVVEGADAVRPIATWDPATSAWAAISPGPVIG"
     gene            931..1989
                     /locus_tag="VHM48_02435"
     CDS             931..1989
                     /locus_tag="VHM48_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754288.1"
                     /translation="MNRVVALILHGRPGPGSGPLTEAFAGIRRRNADRLAERFRLAGA
                     DAVRVIDEAADDRSFGNRLRDLVRASSADGIIVLGSGSVPLARTTDLAAFVATAAGPA
                     GHALANNRFSADLVAIAGIACLAELPNLAADNGLPRWLADGAGYAVEDVRSRWRLQVD
                     LDSPLDVLLCGAPDRMALEAAGVSVDVVRAALQRVAAVGRDPRAELVVAGRASAAGLA
                     WLERASASRTRALIEERGFRTRRDGQRPVRSSLGLLLDRDGPAALGAILAELGDGALI
                     DTRVLLAHRLGADESRWPAAEDRFASDLLLADRIVDPWLRSLTAAARDATIPIVLGGH
                     SLVGPGLRLALGEGRAWT"
     gene            1980..3143
                     /locus_tag="VHM48_02440"
     CDS             1980..3143
                     /locus_tag="VHM48_02440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2754289.1"
                     /translation="MDLRRVPAPARDVASDPALVAAIREEIERAGPMTFARFMEIALY
                     DTARGYYRGVVARPGRAGDFLTAPEASPIFGRTLARFARGVHAAIGAPGAFTIREHGA
                     GTGALAAPLVTALLANDGGPNSIDYLVAEIEPARVEAVRVALADRPRVTVEPDDGRSI
                     DGLVLANEVLDALPTHRVVRRSGRLREILVAIGSAGELTDIEVEPTTATLADRLAAEA
                     VELADGQHAEICVAVDGWIERAAAGLARGVLLLIDYGHPAADLYDPRRRAAGTLATYL
                     GHQVAEDPYRAIGRQDITAHVDISAVERAAVAAGLDHLGTTTQAEFLSRLGAGELLVA
                     EQTGPGASLQGYLETRSALVRMIDPGAMGRFRVMAFGRGLGAGALPPGFEGRS"
     gene            3802..4167
                     /locus_tag="VHM48_02445"
     CDS             3802..4167
                     /locus_tag="VHM48_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX2754290.1"
                     /translation="MTNIWYIVGFMVAGVSFVFLTIGASWLISHRNRGDVHKGLPYES
                     GIDTYGDTHGRYGLSFYIYALLFVAFDIEVVFIYLWAIIFRAVGVDVGSLFISMLAFV
                     GILLLGLAYAWRKGVLTWR"
     gene            4158..4916
                     /gene="nuoB"
                     /locus_tag="VHM48_02450"
     CDS             4158..4916
                     /gene="nuoB"
                     /locus_tag="VHM48_02450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX2754291.1"
                     /translation="MALNEDTGAGLGTGSDDATAAGSDAPGNRPAALIVSQTAVAEPL
                     IVRNTLWRSTDPNAYQGGLEPDVTHLRDVEASGIARRDDARWSGALEVIPTKADYVLD
                     LIRANSLWPLLSGLACCAMEMMSSATSRNDMDRWGMFPFRASPRQADVLIVAGTLTTK
                     MSGPLVRLWEQMPEPKWCVAMGDCTCSGGRYKRSYATIEGIDRVMPVDVYVPGCPPRP
                     EGLIYGMMKLQQLVKDRRGHWPDRAVGPTVPEDV"
     gene            4917..5465
                     /locus_tag="VHM48_02455"
     CDS             4917..5465
                     /locus_tag="VHM48_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2754292.1"
                     /translation="MDAALKRHLHERFGSQLIAPIHQRHDETEIRIGPGDVPDVCRAL
                     HDEPGFGFILLADLAGVDTGDVMQVVYHLWSETSSDWLRIVADGLSREDPRVPSVTFL
                     WQGAEWMEREAYDMFGITFEGNRDLRRIYMPPDYQSFPLRKDFFLADDAARSPGAGVR
                     HMEQSHAPSNARAATMIRKTRA"
     gene            5462..6700
                     /locus_tag="VHM48_02460"
     CDS             5462..6700
                     /locus_tag="VHM48_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HEX2754293.1"
                     /translation="MTTATPHVHPPQEVPSDDPRVQLDEAVGVFEPIPPYPPRTERES
                     EFYTLGDGEMFINMGPQHPSTHGVLRIVLKLDGENVVDLDPVLGYLHRGVEKLCENAD
                     YHHTIAYLDPLEYISSLFCEWPGVLAFEKLLDVEVPRRAEYIRVLSTELNRILSHSLF
                     MGWMALDLGGLTPILWSFIERDEIAEMLAALTGQRLLFNYLRIGGVNGDLNHEFLSRL
                     GDWMSRATTHIESNLGLLNENEIFVRRMRGLGVLDKETAFRMAVTGPNLRASGVPLDF
                     RRAHPYSVFPELEFDIPTRDEGDCLARYLVRIDEIKQSLRIIDQCLHNMPDGPIMAKL
                     PRLLRPRPGRAYAAVEGPRGQYAAYVISDGTDQPFRMRIHDPSFVHLQSVGVLMPGHL
                     IADAMAVMASLDPIMGGVDK"
     gene            6990..8357
                     /locus_tag="VHM48_02465"
     CDS             6990..8357
                     /locus_tag="VHM48_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX2754294.1"
                     /translation="MARSFSRLTLAGKILFTVIPILLVLTGGLIVLIVVAGKPIVDFL
                     ATNIWLVRFVVAATAVLVMSIPSAFLIIYMEMKVIALMNLRIGPDRVGPYGSLLSVVH
                     GLKVLAKEDFSPTGADRLVFTLAPVAVFMATILSLLVLPFAPGLFGADMNIGLLYLFA
                     VSGMTVVGLLMGGWSSFNKYSLIGGLRSAAQVVSYEIPLTLSVVGLLLLAQTMSLNHI
                     VELQGPYKDAAGVVHGGWFTDWFVFRQPLGALIFFIAATAEANRTPFDLTEADSEIVA
                     GFATEYSGMRYGFFYFAEYVNVFLVSGLIVTLFFGGWNGPFPWPWPITLNIDPGSMGL
                     GLLFALLIVPPLLVLALSAPFYVGRSSLKWWQALIIGFGLFNLLVIVAIGGWAFISWD
                     WVGGLLWFLLKSYVFVFVFVWMRGTLPRVRIDQLMGFAWKWLLPASLLNLFVTAAALV
                     VVHPT"
     gene            8368..9087
                     /locus_tag="VHM48_02470"
     CDS             8368..9087
                     /locus_tag="VHM48_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2754295.1"
                     /translation="MSSLPGLGIVKGMGLTLRKFFEPKATIKYPEVRLDVPHKFRGRL
                     QLLYDEYGALKCETCFQCAQACPIECIDMGGIDTKGRYHIHWGAPETYGERREESALR
                     RSGRPVPDQAYAHFDPIHPEGLVAILERFDFDPKRMLEILEATQAEYGHLPVAALKLI
                     SQRTGAWYAMIYGTASYYRHLRFEPSAGEPLAAATAAARAARETTFLAGLDGALAGSS
                     GAARGGGATRAAAPGARPARA"
     gene            9098..10438
                     /locus_tag="VHM48_02475"
     CDS             9098..10438
                     /locus_tag="VHM48_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2754296.1"
                     /translation="MTTILRTPKDWPAILLPGSTRAAAPAADPLGLDAARDRGAFAGL
                     RRSLHDLGATGTIATIAASGLRGRGGAGFPTGEKWRAAAGFPATRRYVVANGYGADPS
                     SATDRTLLERNPYGVLEGAIIAATAIGATEAIVAVRSDATDAIRALEAALERMIAANL
                     AGDDVLASGRSVEVTIRPVQGAYMLGEETVLLKALEGKRGQPEQRPPHPTERGLFGQP
                     TVVQNVQTLAAVPWILANGAEAFAAIGSKASPGTILVSVRAPGGAGVAEVPIGTSLRD
                     IVGLAGPRAAAAELKAFLVGGPSGGILPAELADTAYEYDALRSVGAHIGSGAIVAADK
                     RACIVDLARLLTRFCADEACGKTIPCRIGLRRVSEIGDRIATGLPKPTDVQLLADLSS
                     DIVGSALCDHERLATLPFTSGMRYFRSELDEHILRSSCPAGVCRPIAVAAGAAH"
     gene            10446..13112
                     /locus_tag="VHM48_02480"
     CDS             10446..13112
                     /locus_tag="VHM48_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754297.1"
                     /translation="MTDLITRPDSSSNALAPLDDSLAERLLTTQSPQRPQSTPRIRIE
                     VDGRVVEGFEGQTILEVCRDNGIEIPTLCYEPKLPGFGACRMCVVEVEGEEHPPISCS
                     RTCEPEMVVKTQTEEVRRLRRTNLELIFSDHNAYCLPPCQNKCPSHIDIPGFLKANAE
                     ANFTESTRIFKRTIPFPSVLGRVCPAPCEEHCRRDEVDEAIAIRDSHRYAGDQVIKSM
                     MDDGIDPPVPFELQPKSGRRAAVIGSGPAGMAAAYYLLIAGHDVTVFERDAAPGGMLR
                     YGIPQYRLPKVEVLEAEYESVTRLGGKIVCNAELGKDFGLDDLQNQGFAAVVVAIGCY
                     ATNKLGVPGEDAAEVLDGLEYLRTATLGLPYPGHAGKRVVVVGGGFTSMDCSRTSIRQ
                     GAAEVTLVYRRDMKDMPASGEVHEAIEEGVTAIFQAGPTRVITDDNGKVTGIEFIRMQ
                     PGAPDASGRRRPEPAPGTEFTIPCDRVLLAIGQGPELAWIGPGSEGPVATKQRRLKAD
                     AVTFLTDRPGVFGTGDVRIGAATVVQAIAEGRRSAYAVDAFLKGLDLSAIKTRQQLAE
                     PQPEFLSIVPFTSEQKEPRYRLKAMEAAVRNDSYIEYEIPYSREEVVAESSRCLQCTC
                     EAIGFCDLRRLGIEYGTTLQTLEPQNHQGAGFRSVTENRFTGVNHDYIRDDSHAFILR
                     EPSRCIDCGRCAQVCSEVVGAACYDFMRIGFDTLVTTPLDMSLNDTPCVSCGRCAETC
                     PTGALMPKPRVLQKYEVDESRCILCGICVDACPYDALRDGGDFELSHTSRVDPMIDLM
                     ALASVDQETEMTYIRRERDWAERAAAAGRPVAPTRLLPMLPAAVAAQIAKGETGAGGN
                     GHRHGNGNGHVPALSAGHGPGAAGDHAGHGHG"
     gene            13124..13669
                     /locus_tag="VHM48_02485"
     CDS             13124..13669
                     /locus_tag="VHM48_02485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2754298.1"
                     /translation="MAIGSTIPFTAIPWDDALFVLLAGTMLASGLAVVVMRDIIRCGL
                     AMMVCFGALAGIYVMAGAPLVGAAQVLVYIGAISVLVLFAIMLTQTKSGPARLAFQTQ
                     AVPAAVAAVILLLVVLLAVGSTTWGGGPDRSDTNTADVAKALFGPFVLPFEIVGVLLL
                     AAVIGGVFLAKRDRSPRDGSE"
     gene            13666..14061
                     /gene="nuoK"
                     /locus_tag="VHM48_02490"
     CDS             13666..14061
                     /gene="nuoK"
                     /locus_tag="VHM48_02490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2754299.1"
                     /translation="MIRVFVGSALAAAFIIGLGLIGLAAGGSHSLNAYLTLSGILFTI
                     GMFGFLARRNAISMLMSIELMLNAVNLSIIGFTSFAATPEFRANGSVIALLVMAVAAA
                     EATVGLSIILAIYRNKKTPLVDEYDAMSQ"
     gene            14061..16088
                     /gene="nuoL"
                     /locus_tag="VHM48_02495"
     CDS             14061..16088
                     /gene="nuoL"
                     /locus_tag="VHM48_02495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2754300.1"
                     /translation="MFDLLVPLIVLLPLAGFLFTAAVGRRLGKQAHLVPVGAVGLAWA
                     IGMLVAFSSLTGAAPFGHEGYGHDYYTWIPSGSFQVDVGFFVDNLTACLLIVVLTIGL
                     LVHVYSIGYMSHDPGFWRFFAYLNLFMVSMLLLVLADSFLVVFVAWELVGLSSYLLIG
                     FWKQKRSAALAAKKAFIVNRVGDVGFALGIMAIFVDVGTLNIRDAIAALTGQIATFGQ
                     NPHLVVDGAGLPIPIWVVALLIFAGAMGKSAQFPLHVWLPDAMEGPTPVSALIHAATM
                     VNAGVYLVARTNPIFASAPQSMAVVATIGIFTAILAASIALTQTDIKRVLAYSTLSQL
                     GYMFASLGVGAFSAAIFHLMTHGFFKGLLFLGSGSVIHAVHEEQDMRRMGGLAKRIPH
                     TYRTMLLGSIAIAGIPPLAGFFSKDEILGEAFKNGFQWVWAIGIVVAIMTAFYMFRLI
                     GLTFWGESRVDPAVEPHIHESPSVMTTPLWLLAVPAVLLGLVLSLPGPPIGPLLGLHG
                     PGLLSGWLEPVFKQGSELLGRTEAEFQIFGLDGALLGASVAVALIGVLAAWRLFGVNL
                     GRIQWRPNPERVRALSARVPFLYRASLNKWWFDDLNHLLFMVIGGRIAAFLWWFDRRV
                     VDDTVNDIATTTVGAGTSLRRVQTGRVQNYALGIAVGLIVMAGSFLVLAAR"
     gene            16099..17766
                     /locus_tag="VHM48_02500"
     CDS             16099..17766
                     /locus_tag="VHM48_02500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2754301.1"
                     /translation="MNVDDLPILTIVTFSPLVGALIVAVAPSRYARSMALAASLVAWV
                     VSLLLIVGFDPNAPASRPFQYVQSAPWIPLFGIQYKVGIDGLSLILVVLTTTLTWISI
                     LASFSPIKERIKEYMVSFLILEVGMIGVFVALDTFLFYIFWEIVLVPMYLIIGIWGGT
                     NRIYATIKFVLYTLVGSLLMLVAILATAFAYQSATGSWTGAFDFELLRKAHFDPNLQL
                     FAFLAFFLAFAIKVPMFPFHTWLPDAHVQAPTAGSVILAAIMLKLGAYGFIRFALPLF
                     PAAAHTFGPAIIVLSLIAIIYGAIVALVQPDLKKLVAYSSVSHMGFVTLGIFVFNQQG
                     MDGAILQMVNHGLITGALFLLVGVIYERTHDRTIAKMGGVAAAMPIYAVALGFFVFAS
                     GGLPGLSGFVGEFLVLVGAFEYAPAVAAVATVCMILAAGYLLWMFQRIAFGEVSDFLK
                     GLGHHLTDMTPTEILTLAPLAALTVVFGLFPSLVLDLVKGSVTAVLDDAAREGSIALA
                     TQTAVVLIALPILYVVGRLIWVARIDMSGGGGGGSAVEPVASAEGVS"
     gene            17763..19244
                     /locus_tag="VHM48_02505"
     CDS             17763..19244
                     /locus_tag="VHM48_02505"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2754302.1"
                     /translation="MTTADLNIISPLLAGVLTAIAILVVDLALPNRPTPVLFAALAGL
                     AVTAAVTVSVGSGVATSAFITDGHPMYRLDALTTFLDLLFISIVAMTIVFAPDYLAPR
                     NLPVAEFAVVLVFAMCGAMVIAAAADLLLLFLGLELMVLPGYLLAGYHKSDNFSTEGA
                     IKYFLLGSFSSAIFLFGLTFVWGLSGSTSIAVVASKLTEVATGQAPFSPGLAMGFAFL
                     TTGVAFKIAAVPFHYWTPDAYQGSPTPVTGYLSVGPKIGAFALILRLFVEALGPMKAD
                     WLPIVVVLAALTMTLGNLVALTQDNVKRMLAYSSIAHTGYMLVGLAAYAGGQRQGLEG
                     LLFYGAAYSFMNLGAFAVIAALQKRAGVTSGLATFAGLGRKEPILGLLMALFLLSLTG
                     IPPTAGFFAKALIIVAAVQAGGWVGFLAVLMVLNAAAAAFYYLRVVVYMYMRDPASEA
                     PALRHGRLLWGGLAAATAMTILFGLFPGLLLGFIGDAAKAIAG"
     gene            complement(19454..20149)
                     /locus_tag="VHM48_02510"
     CDS             complement(19454..20149)
                     /locus_tag="VHM48_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2754303.1"
                     /translation="MKTAFAILAGLLVGVLVAAGILAAFVFAGPDPVGLRPTPAPTLV
                     PPSVAPSPSPSAAIASAGPSASAAASSGPGGSPAGSAVPGAAFHIGQPAPALVVPRVG
                     GGTIDLASLRGKAVWLNVMQTTCPECVDEFPLMNGFSARYSDKGLVVIAIDIHEDEGT
                     VASFATRLKAVFPLGLDSDGKAQQTWGTYALPIHFRIDTDGIVRAGALGGVGPDVFVA
                     SLQKILPGVKVTP"
     gene            20219..20947
                     /locus_tag="VHM48_02515"
     CDS             20219..20947
                     /locus_tag="VHM48_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754304.1"
                     /translation="MRTLFGALRDRLLPAVLTAAGVTLIAAGLLSYNGPASAADPSPS
                     AEPTIVSADPGSSLAFPSLPPIDASAPPSSGPSASPNAKRVATRVVVEALGIDLPIIK
                     PRGSSSTYPQCNVALYIQELFQPGQGRATYLYAHARDGMFGPIYERAIQKKSGGPKSM
                     IGMIVQIYTSDDLLYEYAVTEVRLHQLTLDDAIHATTEELWLQTSEGPKGTPGKTQLL
                     AKPLITLPADHKAAHPTPHPVTCA"
     gene            21021..>21730
                     /locus_tag="VHM48_02520"
     CDS             21021..>21730
                     /locus_tag="VHM48_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754305.1"
                     /translation="MRFPVRALRSRLLPALLTAAGVTLLAAGLLHYGGPADARPAGAS
                     PSPSNVAVLPTPGFIVPSLPPIDGSPRPSVAPSPAANRVATRVVIDELGIDLPIIAQP
                     NPRYPSCNVAMYYQDPRLGQPGSGRSIYLYAHARTGMFGPLYERAILGRGGGRKSLIG
                     LPVEVYTSDDQRYVYTITGVRPNVKADSHFLDGPLAVKAETLWLQTSTGPNASYPKLQ
                     VVAQPLGVVPASHAEWLG"
BASE COUNT         3003 a         7957 c         7261 g         3509 t
ORIGIN      
        1 tgttcgacgg gagtgagctc gtcgaggtcg tcacgtcggc gcccgacgcc ggcgcgtggc
       61 gggtggagcc cgacggcgcc ggaggcgcga ccgagaccgc gttgccgacc cggttcctcg
      121 gcgggtgatc gccgcgcaac ccgatccgac gcccatcatc gctcgaacac gatcgccgga
      181 aggccgggtt ccgcttgatc ggcccgcgcg agttcgagta cccgaaggac cacccgatga
      241 cctgcaacga ctgggtcgtc gatccgccgg gatgggtcag ccgatcaccg gccccgggct
      301 gatcgccgcc caggccgacg ttgccggatc ccacgtcgcg atcggtcgga cggcgtcggc
      361 gccctcgaca acgagtcgac gggccgccgc ggtggcgacg gtttcgggcc agcccggcgg
      421 gacgagaccg ggcgcgacat cggccagggg cgcgaggacg aacaggcgct gtgcggtgtc
      481 gcggtggggg acgacgagca gccgatccgc cttgccgggg tccagggaca ggccctccgg
      541 cgggcggtcg agggacaacc gcgcgcggcc gaagatcagc aggtcgagat cgagctcgcg
      601 cggcccccag cgctcgccct cccggcggcc gaacgcgcgc tcgagctgct tgagcgcgat
      661 caggagggcg gtcgcgccgg tggccggatc cgggcccgcc gggacgtcga tcgcgacgac
      721 cgcgttgcgg aacgacggct ggtccaccac gccgaccggt tcggtcatgt acaaccgcga
      781 gaccgcgtgg aggcgggcac cggacagcgc ggccagggca cggacgccgc cggcgagcgt
      841 ccgggccggg tcgccgaggt tggccccaag gccgatatac gcccgtactc gtcgcatcgg
      901 ccggccttcc tcgctcgcta gactcgccgc gtgaaccgcg tcgtcgccct cattctccac
      961 ggccggcccg ggccgggctc gggaccgctg accgaggcgt ttgccgggat ccggcgacgc
     1021 aacgccgacc ggctggccga gaggttccgc ctcgccggcg cggacgcggt ccgggtcatc
     1081 gacgaagcgg ccgacgatcg gtcgttcggg aatcgtctgc gggacctggt tcgtgcgtcg
     1141 agtgcggacg ggatcatcgt gcttggctcg ggcagcgtcc cgctggcccg gacgaccgac
     1201 cttgcggcct tcgtcgcgac cgccgccggt ccggccggcc atgccctcgc caacaatcgc
     1261 ttctcggccg atctcgtcgc gatcgccgga atcgcgtgcc tcgccgagtt gccgaacctc
     1321 gccgccgaca acggactgcc gcgctggctg gccgatggcg ccggatacgc agtcgaggat
     1381 gtccgatcgc gctggcgcct ccaggtcgat ctcgactcgc cactcgacgt cctcctctgc
     1441 ggcgcgccgg accggatggc cctcgaggcg gccggcgttt cggtcgacgt cgttcgggcg
     1501 gccctgcaac gcgtggccgc ggtcggtcgc gatccacggg cggagctcgt cgtcgccggc
     1561 cgcgcgtccg cggccggcct cgcctggctg gagcgggcaa gcgcgtcacg gacccgggcc
     1621 ctgatcgagg agcgtgggtt ccggacccgg cgggacggcc agcggccggt tcgatcgagc
     1681 cttggcctgc tcctcgaccg cgacgggccg gcggccttgg gcgcgatcct ggccgagctc
     1741 ggcgacgggg ccctgatcga tacgcgggtc ttgctcgccc atcgactcgg cgccgacgag
     1801 agccgctggc ccgccgccga ggatcgcttc gcgagcgatc tcctgcttgc ggatcggatc
     1861 gtcgatccgt ggctgcgatc gctcactgcg gcggctcgcg atgccaccat cccgatcgtg
     1921 ctcggcggcc acagcctcgt cgggccgggg ctgcggctcg cgctcgggga gggtcgtgcg
     1981 tggacctgag acgcgttccc gcgccggccc gggatgtcgc gtcggatccc gcgctcgtgg
     2041 ccgccatccg cgaggagatc gagcgagccg ggccgatgac gttcgcccga ttcatggaga
     2101 tcgcgctcta cgacacggcg cgtggctact accggggagt cgtcgcccgg cccggccgcg
     2161 cgggggattt cctgaccgcc cccgaagcga gcccgatctt cggccggacg ctcgcccggt
     2221 tcgcccgtgg agtccatgcg gcgatcggcg cgccgggcgc cttcacgatc cgcgagcacg
     2281 gggcggggac cggcgcgctg gcggcgccgc tcgtgacggc gctgctcgcg aacgacggtg
     2341 ggcccaattc gatcgactac ctcgtcgcgg agatcgagcc ggcccgggtc gaggcggtgc
     2401 gcgtcgcgct cgccgatcga ccgcgggtca cggtcgagcc cgacgacggc cgttcaatcg
     2461 acggcctggt tctcgccaac gaggtgctcg acgccctgcc gactcatcgg gtcgttcgcc
     2521 gcagcgggcg tctccgcgaa attctcgtcg cgatcggatc agccggcgag ctcaccgaca
     2581 tcgaggtcga gccgacgaca gcgaccctcg ccgatcgtct cgcggccgag gcggtcgagc
     2641 tggccgacgg tcagcacgcc gagatctgtg tcgcggtcga cggatggatc gagcgggccg
     2701 ccgccggact ggccaggggc gtcctgctcc tgatcgacta cggccacccg gccgcggacc
     2761 tctacgaccc gcgacgtcga gcggccggca cgctggcgac gtatctcggc caccaggtcg
     2821 ccgaggatcc gtaccgggcg atcgggcgcc aggacatcac ggctcatgtc gatatcagtg
     2881 ccgtcgagcg ggcggcggtc gcggccggtc tcgaccatct cggcaccacc acccaggccg
     2941 agttcctgag ccggctgggc gcgggcgagc tgctggtcgc cgagcagacc gggccgggcg
     3001 cgagcctgca gggctacctc gagacccgct cggcgctcgt ccggatgatc gatccggggg
     3061 cgatgggccg cttccgggtg atggcgttcg ggcgcgggct cggggctggc gccctgcccc
     3121 cgggattcga ggggcggtcg tagccgggcg gctctcccgc gcgcccaatc cggccagatc
     3181 gtcgcccaga gactcttggg acgactcgac gccctctggg aagcgattcg ggtcgccgcg
     3241 ttagcgtttc gagggctaat ccgaccccgc cggccgcccg agtgagcagt gtttggccgg
     3301 cgagttcgcc catgagtctc cccggaaata tctggccagg tccgggccaa cttcagcggc
     3361 caccagtcgt cgcgccggcg gcggtccgag ggcttgagca ggcgactagg gcgaattccc
     3421 tattgccgtg agacccggcc gaggaagagg ctgcgcttgt cccggcgacg gggcacaccg
     3481 ccggggagcg gatcgtggac tggcacgcgc gctcggcacg gtccgctccc cgatcacgct
     3541 cgtcgccgac ccaccggacg ccgctcgccg aggctgccaa gccttgtcgc ggagccgcat
     3601 ccggacgtga tcccgcccta ccgaggtccg tggcgcagga cgtccggcag tcccgagcgc
     3661 gcggtggacg agcgggtccc cctgctagta ttgggccgcg tgacgagcgt tcgtcggagt
     3721 gcatgacgcg cgcaggggac catcccgggt gctccagccg ggttcggtcg acccgacagc
     3781 ccaaccggag agaccgtcga cgtgaccaac atctggtaca tcgtgggatt catggtcgcc
     3841 ggcgtgtcgt tcgtcttcct gacgatcggg gcgtcctggc tgatctccca tcggaaccgc
     3901 ggcgacgtcc acaagggcct gccgtacgag tccgggatcg acacctacgg cgacacccat
     3961 ggccggtacg gcttgtcgtt ctacatctac gccctgctct tcgtcgcctt cgacatcgag
     4021 gtcgtcttca tctacctctg ggcgatcatc ttccgcgccg tcggcgtcga cgtcggctcg
     4081 ctgttcatca gcatgctcgc cttcgtcggc atcctgctcc tggggcttgc ctacgcctgg
     4141 cgcaaggggg tcctgacatg gcgctgaacg aggacaccgg ggccggcctc gggaccggat
     4201 cggatgacgc gaccgccgcc ggctcggatg cgccgggcaa ccggccggcc gccctgatcg
     4261 tcagtcagac ggcggttgcc gagccgctca tcgtccgcaa caccctgtgg cgctcgaccg
     4321 acccgaacgc ctaccagggc ggcctcgagc cggacgtcac ccacctccgc gatgtcgagg
     4381 cgtcggggat cgcccggcgc gacgacgccc gctggtcggg cgccctcgag gtcatcccga
     4441 ccaaggccga ctacgtcctc gacctgatcc gggcgaacag cctgtggccg ctcctgtcgg
     4501 gccttgcctg ctgcgcgatg gagatgatgt cgagcgcaac ctcccgcaac gacatggatc
     4561 gctgggggat gttccccttc cgggccagcc cccgccaggc ggacgtcctg atcgtcgccg
     4621 gcaccctcac gacgaagatg tcggggccgc tcgtccggct gtgggagcag atgcccgagc
     4681 ccaagtggtg cgtcgcgatg ggcgactgca cgtgctccgg cggccgttac aagcggagct
     4741 atgcgacgat cgaggggatc gaccgggtga tgccggtcga cgtctacgtc ccgggttgcc
     4801 cgccccgtcc cgagggcctg atctacggga tgatgaagct ccagcagctc gtcaaggacc
     4861 gccgcggcca ctggccggac cgggcggtcg ggccgaccgt gcccgaggac gtctaggtgg
     4921 acgcagcgct caagcgccac ctccacgagc ggttcggcag ccagctgatc gcgccgatcc
     4981 atcagcgcca cgacgagacc gagatccgga tcgggccggg cgacgtgccc gatgtctgcc
     5041 gggccctgca cgacgagcca gggttcggct tcatcctcct cgccgacctg gccggggtcg
     5101 ataccggcga cgtgatgcag gtcgtgtacc acctctggag cgagacgagc agcgactggc
     5161 tgcggatcgt cgccgacggc ctgtcgcgtg aggatccccg cgtcccctcg gtcaccttcc
     5221 tgtggcaggg cgccgagtgg atggagcgcg aggcctacga catgttcggc atcacgttcg
     5281 agggcaaccg cgacctccgc cggatctaca tgccgcccga ctaccagagc ttcccgctcc
     5341 gcaaggactt cttcctcgcc gacgacgccg ctcgctcgcc cggcgccggc gtccgccaca
     5401 tggagcagtc gcacgcgccg agcaacgccc gcgccgcgac gatgatccgg aagacccggg
     5461 catgacgacg gcgacgcccc acgtccatcc gccccaggag gtcccgtcgg acgacccgcg
     5521 ggtccagctc gacgaggccg tcggggtctt cgagccgatc ccgccgtacc cgccgcggac
     5581 cgagcgcgag tccgagttct acaccctcgg cgacggcgag atgttcatca acatgggtcc
     5641 ccagcacccc tcgacccacg gtgtgctgcg gatcgtcctc aagctcgacg gcgagaatgt
     5701 cgtcgatctc gatccggtcc tcggctatct ccatcgcggc gtcgaaaagc tctgcgagaa
     5761 cgccgactac caccatacga tcgcgtatct ggacccgctc gagtacatca gctcgctgtt
     5821 ctgcgaatgg ccgggcgtcc tcgcgttcga gaagctgctc gatgtcgagg tgccccgccg
     5881 ggccgagtac atccgggtcc tctcgaccga gctgaaccgg atcctgtcgc actcgctgtt
     5941 catgggctgg atggcgctcg accttggcgg cctcaccccg atcctgtgga gcttcatcga
     6001 gcgcgacgag atcgccgaga tgctcgccgc cctgaccggc cagcggctgc tcttcaacta
     6061 cctccggatc ggcggggtca acggcgacct gaaccacgag ttcctgagcc gcctcggcga
     6121 ctggatgagc cgggcgacga cccatatcga gagcaacctc gggctcctca acgagaacga
     6181 gatcttcgtc cggcggatgc gcggcctcgg cgtcctcgac aaggagaccg cgttccggat
     6241 ggccgtgacc ggcccgaacc tacgggcgag cggcgtcccc ctcgacttcc ggcgggccca
     6301 tccgtacagc gtcttccccg agctcgaatt cgacatcccg acccgcgacg agggcgactg
     6361 cctcgcccgc tacctcgtcc ggatcgacga gatcaagcag agcctcagga tcatcgacca
     6421 gtgcctccac aacatgccgg acggcccgat catggccaag ctcccgcggc tgctccgccc
     6481 gcgacccggc cgggcctatg ccgcggtcga ggggccgcgc ggccagtacg ccgcgtacgt
     6541 gatcagcgac ggcaccgacc aaccgttccg gatgcggatc cacgacccat cgttcgtcca
     6601 tctccagtcg gttggcgtgc tgatgccggg ccacctgatc gccgacgcga tggccgtcat
     6661 ggcttcactg gatcccatca tgggcggggt ggataagtga cggccatcac ggcacgtctc
     6721 ctcccgtcgg cctcgccgag tgtccgtgtg acgggcgtta cgggcgacag atacgagcgg
     6781 atggctcgat cacgtgactt cgttagccgc cgagggcaga tccccgtcaa gaatcccgtc
     6841 gaccgcggcc ggccggcacg gcatgtcgtc gttagcaccc gcacggcggg cattcggcaa
     6901 tttcaaggcg cggcggaggc caccgcggcc gaggctaccg cgggccggct cgccggagcc
     6961 gcccgcgccg gagtccgacc gtgacagccg tggctcgctc gttctcgcgc ctgaccctcg
     7021 ccgggaagat cctgttcacg gtcatcccga tcctgctcgt cctgacgggc ggcctgatcg
     7081 tcctgatcgt cgtcgcgggc aagccgatcg tcgacttcct cgccacgaac atctggctcg
     7141 tccggttcgt cgtggcggcg accgccgtcc tggtcatgag catcccgagc gcgttcctga
     7201 tcatctacat ggagatgaag gtcatcgccc tgatgaacct ccggatcggg ccggaccggg
     7261 tcgggccgta cggatcgctc ctgtcggtcg tccacgggct caaggtcctg gcgaaggagg
     7321 acttcagccc gaccggcgcc gatcgactcg tcttcaccct cgccccggtc gcggtcttca
     7381 tggcgacgat cctgtcgctc ctcgtgctgc cgttcgcgcc gggcctgttc ggggccgaca
     7441 tgaacatcgg cctgctctac ctgttcgcgg tcagcggaat gacggtggtc gggctgctca
     7501 tgggcggctg gtcgagcttc aacaagtact cactcatcgg cggactccgc tccgccgccc
     7561 aggtcgtcag ctacgagatc ccgctgacgc tgtcggtcgt cggcctgctt ctcctggccc
     7621 agacgatgag cctcaaccac atcgtcgagc tgcagggtcc gtacaaggac gcggcggggg
     7681 tcgtccacgg cggctggttc accgactggt tcgtcttccg gcagccgctc ggcgcgctga
     7741 tcttcttcat cgccgcgacc gccgaggcca accggacgcc gttcgacctg accgaggccg
     7801 acagcgagat cgttgccggc ttcgcgaccg agtactcggg catgcgctac gggttcttct
     7861 acttcgccga gtacgtcaac gtgttcctcg tctcgggcct gatcgtgacc ctcttcttcg
     7921 gcggctggaa cgggccgttc ccgtggccct ggccgatcac cctgaacatc gatcccggct
     7981 cgatgggact cggcctcctg ttcgccctgc tgatcgtgcc gccgttgctg gtcctcgccc
     8041 tgtccgcgcc gttctatgtc ggccggagca gcctgaagtg gtggcaggcg ctgatcatcg
     8101 gcttcggcct gttcaacctg ctcgtcatcg tcgcgatcgg cggctgggcg ttcatcagct
     8161 gggactgggt cgggggcctg ctctggttcc tcctgaagag ctacgtcttc gtcttcgtct
     8221 tcgtctggat gcgcggcacg ctgccccgcg tccggatcga ccagttgatg gggttcgcct
     8281 ggaagtggct cctgccggcg tcgctgctga acctgttcgt gaccgccgcc gcgctcgtcg
     8341 tggtccatcc gacgtgaggt ccatgcgatg agcagcctgc ctggtctcgg catcgtcaag
     8401 ggcatgggcc tgacgctccg caagttcttc gagccaaagg cgacgatcaa gtaccccgag
     8461 gtgcggctcg acgtcccgca caagttccgc ggccgcctcc agctgctcta cgacgagtac
     8521 ggcgcgctga agtgcgagac ctgcttccag tgcgcccagg cctgtccgat cgagtgcatc
     8581 gacatgggcg ggatcgacac gaaggggcgc taccacatcc attggggcgc gcccgagacc
     8641 tacggggagc ggcgcgagga atcggccctc cggcgttccg gccggcccgt tccggaccag
     8701 gcctacgccc atttcgaccc gatccatccc gagggcctcg tggcgatcct cgagcgcttc
     8761 gatttcgatc cgaagcggat gctcgagatc ctcgaagcga cccaggccga gtacggccac
     8821 ctgccggtcg cggccctcaa gctgatcagc cagaggaccg gggcgtggta cgcgatgatc
     8881 tacgggaccg cgtcctatta ccgccacctc cggttcgagc cgtcggccgg cgagcccctc
     8941 gcggcggcga ccgccgctgc ccgggccgcg cgcgagacga cgttcctcgc cggtctcgat
     9001 ggcgcgctgg ccggatcatc cggggcggca cggggcggtg gcgcgacacg ggccgcggca
     9061 ccgggcgcac gcccggcacg agcctgagcg cggaccgatg acgacgatcc tccgaacgcc
     9121 gaaggactgg ccggcgatcc tcctgcccgg gagcactcgc gccgcggcgc cggccgcgga
     9181 cccgctcggc ctcgacgcgg cccgcgatcg aggcgcgttc gccggactcc ggcgatcgct
     9241 ccatgacctc ggcgcgaccg gcacgatcgc gacgatcgcc gcctccgggc tgcgtggacg
     9301 tggcggcgcc ggcttcccga ccggcgagaa gtggcgagcc gccgccggct tccccgcgac
     9361 gcgacgctac gtcgtcgcca acggctatgg agcggacccc tcgtcggcga cggaccggac
     9421 cctcctcgag cgcaatccgt acggcgtcct cgagggcgcg atcatcgccg cgaccgcgat
     9481 cggggcgacc gaggcgatcg tcgccgtccg gtccgacgca acggacgcga tccgggccct
     9541 cgaggcggcc ctcgaacgga tgatcgcggc caatctcgcc ggcgacgacg tcctcgcctc
     9601 cggccgttcg gtcgaggtca cgatccggcc ggtccagggc gcctacatgc tcggcgagga
     9661 gacggtcctc ctcaaggcgc tggaaggcaa acgcggccag cccgagcagc ggcccccgca
     9721 cccgacggag cgcggcctgt tcggccagcc gaccgtcgtc cagaacgtgc agaccctcgc
     9781 ggccgttccg tggatcctgg ccaacggcgc cgaggcgttc gccgcgatcg gctcgaaggc
     9841 cagcccgggc accatccttg tctccgtccg ggcgcccggc ggggccggcg tggccgaggt
     9901 tccgatcggg acatcgctca gggacatcgt cggcctcgcc gggccgcgcg ccgcagcggc
     9961 cgagctcaag gcgttcctcg tcggcggccc gtcgggtggg atcctgccgg ccgagctcgc
    10021 ggacaccgcc tacgagtacg acgccctccg ctcggtcggc gcccacatcg gctcgggcgc
    10081 gatcgtcgct gccgacaaac gcgcctgcat cgtcgacctg gcccgcctcc tgacccgttt
    10141 ctgcgccgac gaggcgtgcg gcaagacgat cccctgccgg atcgggctcc gccgcgtcag
    10201 cgagatcggc gaccggatcg cgacgggcct gccgaagccg accgacgtcc agctcctggc
    10261 cgatctgtcg agcgatatcg tcggctccgc cctgtgcgac cacgagcgcc tggcgaccct
    10321 tccgttcacg agcgggatgc gatacttccg ttccgagctc gacgagcaca tcctgcggag
    10381 ctcgtgcccg gccggcgtct gccgaccgat cgcggtcgcc gctggcgcag cccactgacg
    10441 agacgatgac cgacctgatc acccgccccg acagcagctc caacgccctc gccccgctcg
    10501 acgattcgct ggcggagcgc ctcctgacga cccagtcgcc gcagcgaccg cagtcgacgc
    10561 cgcggatccg gatcgaggtc gacggccggg tcgtcgaagg cttcgagggc cagacgatcc
    10621 tcgaggtctg ccgcgacaac gggatcgaga tcccgaccct ctgctacgag ccgaagctgc
    10681 ccggtttcgg cgcctgccgg atgtgcgtcg tcgaggtcga gggcgaggag catccgccga
    10741 tctcgtgctc gcggacatgc gagccggaga tggtcgtcaa gacccagacc gaggaggtcc
    10801 gccgcctccg ccggacgaac ctcgagctga tcttcagcga ccacaacgcc tactgtctgc
    10861 cgccctgcca gaacaagtgc ccgagccaca tcgacatccc gggcttcctc aaggcgaacg
    10921 ccgaggcgaa cttcaccgaa tcgacccgga tcttcaagcg cacgatcccg ttcccgagcg
    10981 tcctcggccg ggtctgcccg gcgccgtgcg aggagcattg ccggcgcgac gaggtcgacg
    11041 aggcgatcgc catccgcgac tcgcaccgct acgccggcga ccaggtgatc aagtcgatga
    11101 tggacgatgg catcgacccg cccgtcccgt tcgagctcca gccgaagtcc ggccgccgcg
    11161 cggcggtcat cggctccggg ccggccggca tggccgcggc ctactacctc ctgatcgccg
    11221 gccacgacgt gaccgtcttc gagcgtgacg cggcccccgg cgggatgctc cgctacggca
    11281 tcccccagta ccgcctgccg aaggtcgagg tcctcgaggc cgagtacgag tcggtgaccc
    11341 ggctcggcgg caagatcgtc tgcaacgccg agctcggcaa ggacttcggg ctcgacgacc
    11401 tccagaacca gggcttcgcc gcggtggtcg tggcgatcgg ctgctacgcc acgaacaagc
    11461 tcggcgtccc gggcgaggac gcggccgagg tcctcgacgg cctcgagtac ctccggaccg
    11521 cgaccctcgg cctgccctac ccgggccacg ccggcaagcg cgtggtcgtc gtcggtggcg
    11581 gcttcacgtc gatggactgc agccggacct cgatccgcca gggcgcggcc gaagtgaccc
    11641 tcgtctaccg ccgcgacatg aaggacatgc ccgcgtcggg cgaggtccac gaggcgatcg
    11701 aggaaggcgt caccgccatc ttccaggccg gcccgacgcg ggtcatcacc gacgacaatg
    11761 gcaaggtcac cggcatcgag ttcatccgga tgcagccggg cgcgcccgac gcgtccggcc
    11821 ggcgccggcc ggagcccgcg cccgggaccg agttcacgat cccctgcgac cgggtcctcc
    11881 tcgcgatcgg ccagggcccg gagctggcgt ggatcggccc ggggtccgag ggcccggtgg
    11941 ccaccaagca gcgccgcctc aaggccgacg ccgtcacgtt cctgaccgac cggcccggcg
    12001 tcttcgggac cggcgacgtc cggatcggcg ccgcgaccgt cgtccaggcc atcgccgagg
    12061 gccgccgctc agcctacgcg gtcgacgcct tcctcaaggg cctcgacctg tccgcgatca
    12121 agacccgcca gcagctcgcc gagccgcagc cggagttcct ctcgatcgtg ccgttcacga
    12181 gcgagcagaa ggagccgcgc tatcgcctca aggcgatgga ggcggccgtc cgcaacgaca
    12241 gctacattga gtacgagatc ccgtacagcc gcgaggaggt cgtcgccgag tcgtcgcgct
    12301 gcctccagtg cacctgcgag gcgatcgggt tctgcgacct gcgccgcctc gggatcgagt
    12361 acgggacgac gctccagacg ctcgagccgc agaaccacca gggagccggc ttccgctcgg
    12421 tgaccgagaa ccgcttcacc ggcgtcaacc acgactacat ccgcgacgat tcgcatgcgt
    12481 tcatcctgcg cgagccgtcg cgctgcatcg actgcggccg ctgcgcccag gtctgctccg
    12541 aggtcgtcgg tgcggcctgt tacgacttca tgcggatcgg cttcgacacg ctcgtcacca
    12601 cgccgctcga catgagcctc aacgacaccc cgtgcgtctc ctgcggtcgc tgcgccgaga
    12661 cctgcccgac cggcgcgctc atgccgaagc cgcgcgtcct ccagaagtac gaggtcgacg
    12721 agagccgctg catcctgtgc gggatctgcg tcgacgcctg cccctacgac gcgctccgcg
    12781 acggcggcga cttcgagctc tcgcacacct cgcgggtcga cccgatgatc gacctgatgg
    12841 ccctcgcctc ggtcgaccag gagaccgaga tgacctacat ccggcgcgag cgggactggg
    12901 ccgagcgggc cgctgcggcg ggccggccgg tcgccccgac ccggcttctg ccgatgctcc
    12961 cggccgccgt cgcggcccag atcgcgaagg gcgaaaccgg cgcgggcggc aacggccacc
    13021 gtcacggcaa cggcaacggc catgtcccgg cgctcagcgc gggccacggc ccgggtgctg
    13081 ccggcgacca cgccggccac gggcacggct gagcgagccg ccagtggcga tcggatcgac
    13141 gatccccttc accgcgatcc cctgggacga cgccctgttc gtcctcctgg ccggcacgat
    13201 gctggcgtcg ggcctcgcgg tcgtcgtcat gcgcgacatc atccgttgcg gtcttgcgat
    13261 gatggtctgc ttcggcgccc tggccgggat ctacgtcatg gccggcgcgc cgctcgtcgg
    13321 cgcggcgcag gtcctcgtct acatcggcgc gatcagcgtc ctcgtcctct tcgcgatcat
    13381 gctgacccag acgaaatcgg gcccggccag gctggccttc cagacccagg ccgtccccgc
    13441 ggccgtcgcc gccgtcatcc tcctgctggt cgtcctgctc gcggtcggtt cgacgacctg
    13501 gggcggcggc cccgatcgca gcgacaccaa taccgctgat gtggcgaaag ccctgttcgg
    13561 cccgttcgtc ctgcccttcg agattgtcgg cgtgctgctg ctcgcggcgg tcatcggcgg
    13621 ggtcttcctc gccaaacggg atcgctcgcc ccgggatgga tccgaatgat ccgggtcttc
    13681 gtcgggtcgg cgctcgctgc ggcgttcatc atcggcctcg ggctgatcgg gctcgccgcc
    13741 ggcggatcgc actcgctcaa cgcctacctg accctgtcgg ggatcctgtt cacgatcggg
    13801 atgttcggct tcctcgcccg gcgcaacgcg atcagcatgc tgatgtcgat cgagctgatg
    13861 ctcaacgcgg tcaacctctc gatcatcggc ttcacgtcgt tcgccgcgac cccggagttc
    13921 cgggccaacg gctcggtcat cgccctgctc gtcatggccg tcgccgccgc tgaggcgacg
    13981 gtcggcctgt cgatcatcct cgcgatctac cgcaacaaga agaccccgct cgtcgacgag
    14041 tacgacgcga tgagccagtg atgttcgacc tgctcgtccc cctgatcgtc ctcctgccgc
    14101 tggcgggctt cctgttcacg gcggcggtcg ggcgtcgcct cggcaagcag gcccacctgg
    14161 tcccggtcgg agcggtcggg ctggcctggg cgatcggcat gctcgtggcg ttcagctcgc
    14221 tcaccggggc agcgccgttc ggccacgagg gctacggcca cgactactac acgtggatcc
    14281 cgtcgggctc cttccaggtc gatgtcggct tcttcgtcga caacctgacc gcctgcctgc
    14341 tgatcgtcgt cctgacgatc ggcctgctcg tccacgtcta ctcgatcggc tacatgagcc
    14401 atgacccggg cttctggcgg ttcttcgcct acctgaacct gttcatggtc tcgatgctcc
    14461 tgctcgtcct ggcggacagc ttcctcgtcg tcttcgtcgc ctgggagctg gtcgggctat
    14521 cgagctacct cctgatcgga ttctggaaac agaagcggtc ggccgcgttg gcggccaaga
    14581 aggcgttcat cgtcaaccgc gttggtgacg tcgggttcgc gctcgggatc atggcgatct
    14641 tcgtcgacgt cgggacgctg aacatccgcg acgcgatcgc ggcgctgacc ggccagatcg
    14701 cgaccttcgg ccagaaccct cacctggtcg tggacggcgc cggcctgccg atcccgatct
    14761 gggtcgtcgc cctcctgatc ttcgccgggg cgatgggcaa gagcgcccag ttcccgctcc
    14821 acgtctggct gccggacgcg atggagggcc cgaccccggt gtcggccctg atccacgccg
    14881 cgacgatggt caacgccggg gtctacctcg tcgcccggac gaacccgatc ttcgccagcg
    14941 cgccgcagtc gatggcggtc gtcgcgacga tcggcatctt cacggcgatc ctggccgcgt
    15001 cgatcgcgct gacccagacc gacatcaaac gggtgctggc ctattcgacg ctgtcccagc
    15061 tcggctacat gttcgcctcg ctcggggtcg gggcgttcag cgcggcgatc ttccacctga
    15121 tgacccacgg cttcttcaag ggcctcctgt tcctcggctc gggatcggtg atccacgcgg
    15181 tccacgagga gcaggacatg cgccggatgg gcgggctcgc gaagcggatc ccgcacacct
    15241 accggacgat gctgctcggc tcgatcgcga tcgccgggat cccgccgttg gccggcttct
    15301 tcagcaagga cgagatcctc ggcgaggcgt tcaagaacgg cttccagtgg gtctgggcga
    15361 tcgggatcgt cgtcgcgatc atgaccgcgt tctacatgtt ccggctcatc gggttgacct
    15421 tctggggcga gagccgggtc gatccggcgg tcgagccgca catccacgag tcgccgtcgg
    15481 tgatgacgac gccgctctgg ctcctggccg tgccggcggt cctgctcggg ctcgtgctgt
    15541 cgctgccggg cccgccgatc gggccgctgc tcggcctgca tgggccgggc ctgctcagcg
    15601 gatggctgga gccggtcttc aagcagggct ccgagctgct cggccggacc gaggcggagt
    15661 tccagatctt cggcctcgac ggtgcgctgc tcggggccag cgtcgcggtc gcgctgatcg
    15721 gcgtgctcgc ggcgtggcgc ctgttcgggg tcaacctcgg caggatccag tggcgcccga
    15781 acccggagcg cgtccgcgcg ctcagtgccc gcgtcccgtt cctctaccgt gcatcgctga
    15841 acaagtggtg gttcgacgac ctcaaccacc tgttgttcat ggtcatcggc gggcggatcg
    15901 ccgcgttcct gtggtggttc gatcgacggg tcgtcgacga caccgtcaac gacatcgcga
    15961 ccacgaccgt cggggccggg acgagcctcc gccgcgtcca gaccggtcgc gtccagaact
    16021 acgccctcgg gatcgcggtc gggctgatcg tcatggccgg ctcgttcctc gtcctggccg
    16081 cccgatgagc accctgcgat gaacgtcgac gacctcccga tcctgacgat cgtcacgttc
    16141 agcccgctcg tcggcgccct catcgtggcg gtcgcgccgt cgcgttatgc ccgctcaatg
    16201 gccctcgcgg cgagtctcgt cgcgtgggtc gtgtcgcttc tcctgatcgt cggcttcgac
    16261 ccgaacgccc cggccagccg gcccttccaa tacgtccaat ccgcgccgtg gatccccctg
    16321 ttcgggatcc agtacaaggt cggcatcgac ggcctgtcgc tgatcctcgt ggtcctgacg
    16381 acgaccctga cctggatcag catcctcgcg tcgttcagcc cgatcaagga acggatcaag
    16441 gagtacatgg tctccttcct gatcctcgag gtcgggatga tcggggtgtt cgtcgccctc
    16501 gacacgttcc tcttctacat cttctgggag atcgtcctcg tcccgatgta cctgatcatc
    16561 gggatctggg gcgggacgaa ccggatctac gccacgatca agttcgtcct ctacaccctg
    16621 gtcgggtcgc tcctgatgct ggtggcgatc ctcgcgaccg cgttcgctta ccagtcggcg
    16681 accggaagct ggacgggcgc cttcgacttc gagctcctgc gcaaggcaca cttcgacccg
    16741 aacctccagc tcttcgcctt cctcgccttc ttcctcgcgt tcgcgatcaa ggtcccgatg
    16801 ttcccgttcc atacctggct gccggacgcc catgtccagg cgccgacggc gggctcggtg
    16861 atcctggccg ccatcatgct gaagctgggc gcatacgggt tcatccggtt cgccctgccg
    16921 ctgttccccg cggccgccca cacgttcggc ccggcgatca tcgtcctgag cctgatcgcg
    16981 atcatctacg gcgcgatcgt cgccctcgtc caacccgacc tcaagaagct cgtcgcgtac
    17041 agctccgtct cgcacatggg cttcgtcacg ctcggaatct tcgtcttcaa ccagcagggc
    17101 atggacggcg cgatcctcca gatggtcaac cacggcctga tcacgggcgc cctgttcctc
    17161 ctggtcgggg tgatctacga gcgaacccat gaccggacga tcgcgaagat gggcggcgtc
    17221 gccgcagcca tgccgatcta cgcggtcgcc ctcggcttct tcgtcttcgc ctccggcggc
    17281 ctgcccggcc tgtccggctt cgtcggtgag ttcctcgtcc tcgtcggtgc cttcgagtac
    17341 gcccccgccg tcgccgcggt cgcgaccgtc tgcatgatcc tcgccgctgg ctatctgctc
    17401 tggatgttcc agcggatcgc attcggcgag gtctccgact tcctcaaggg cctcggccac
    17461 cacctgaccg acatgacccc gaccgagatc ctgaccctcg ccccgctcgc cgccctgacc
    17521 gtcgtgttcg gactcttccc gagcctggtc ctggacctgg tcaagggttc ggtgacggcg
    17581 gtcctcgatg acgccgcccg cgagggctcg atcgcgctcg cgacgcagac cgcggtggtc
    17641 ctcatcgcgt tgccgatcct gtacgtcgtc ggccgcctga tctgggtcgc ccggatcgac
    17701 atgtccggtg gcggcggggg cggctcggcc gtcgagcccg tcgcgtcggc cgagggcgtc
    17761 tcgtgacgac cgccgacctc aacatcatct cgccgctcct ggccggggtc ctgaccgcga
    17821 tcgcgatcct ggtcgtcgac ctggcgcttc ccaaccgccc gacgccggtc ctcttcgccg
    17881 ccctcgcggg cctggcggtc accgctgccg tcacggtgtc ggtcggatcc ggggtagcga
    17941 cctctgcctt catcaccgac ggccatccga tgtaccggct cgacgcgctg acgacattcc
    18001 tcgatctgct cttcatctcg atcgtggcga tgacgatcgt cttcgccccg gactacctgg
    18061 cgccgcgcaa cctgccggtc gcggagttcg cggtcgtcct cgtgttcgcg atgtgcgggg
    18121 cgatggtgat cgcggcagcg gcggacctgc tgctgctgtt cctcggcctc gagctgatgg
    18181 tcctgccggg ctatctgctc gccggctacc acaagtccga caacttctcg accgagggcg
    18241 cgatcaagta cttcctgctc ggctcgttca gctcggcgat cttcctgttc gggctgacgt
    18301 tcgtctgggg cctgtccggc tcgacgagca tcgccgtcgt cgcgagcaag ctgaccgagg
    18361 tcgcgaccgg ccaggcgcca ttcagcccgg gcctcgcgat gggcttcgcc ttcctcacga
    18421 ccggcgtcgc gttcaagatc gcggccgtgc cgttccacta ctggacaccg gacgcgtacc
    18481 agggctcgcc gacgccggtc accggctatc tatcggtcgg cccgaagatt ggtgccttcg
    18541 cgctgatcct gcggctgttc gtcgaggcgc tcggaccgat gaaggccgac tggctgccga
    18601 tcgtcgtcgt cctcgccgcg ttgacgatga ccctcggcaa cctcgtcgcg ctgacccagg
    18661 acaacgtcaa gcggatgctc gcctacagct cgatcgccca caccggctac atgctggtcg
    18721 ggctcgcggc gtacgccggc gggcagcgcc agggtctcga ggggctgctg ttctacggcg
    18781 cggcctactc gttcatgaac ctcggcgcgt tcgccgtgat cgcagcgctc cagaagcggg
    18841 ccggcgtgac gtccgggctg gcgacgttcg cggggctcgg ccggaaggag ccgatcctcg
    18901 ggctcctcat ggccctgttc ctcctgtcgc tgaccggcat cccgccgact gcgggcttct
    18961 tcgccaaggc gctgatcatc gtcgctgcgg tccaggccgg cggctgggtc gggttcctgg
    19021 ccgtcctgat ggtcctcaat gcggccgcgg ctgcgttcta ctacctccgc gtcgtcgtct
    19081 acatgtacat gcgggacccc gccagcgagg cgccagccct ccgccacggc cgcctgctgt
    19141 ggggcgggct tgccgcggcg accgcaatga cgatcctgtt cgggctgttc ccgggcctgc
    19201 tcctcgggtt catcggggac gccgcgaagg cgatcgcggg ctagttcggc tcaaggcccg
    19261 tgcccaggcc gggccgcatc tccccatgcg acagccaggc tgtcgttggc gcgatcgcga
    19321 cgcctgtttc ggctgaccgt tagccctcgg cggttgactc atgcggcact gccgccgatt
    19381 cgggcgccgt ccgctcggat cgtcgcgcca gtgccacccc ccgttcgcgt atgggcaaga
    19441 cgcgtcaccg gagctacggc gtgaccttga cccccggcag gatcttctgg aggctcgcca
    19501 cgaagacatc cgggccgacg ccgcccagcg cacccgccct gacgatgccg tcggtgtcga
    19561 tcctgaagtg gatcggcagc gcgtacgtgc cccacgtctg ctgggccttg ccgtcgctgt
    19621 cgagcccgag cgggaacacc gccttgagcc gggtcgcgaa gctcgcgacg gtgccctcgt
    19681 cctcgtggat gtcgatcgcg atcacgacga ggcccttgtc cgagtagcgc gcggagaagc
    19741 cgttcatcag tgggaactcg tcgacgcact ccgggcaggt cgtctgcatg acgttcaacc
    19801 agacggcctt cccgcggagg ctcgcgaggt cgatcgtgcc accgccgacc cggggcacga
    19861 cgagggccgg ggccggctgg ccgatgtgga acgcggcgcc cggaacggcg ctcccggccg
    19921 gcgagccgcc cggtcccgac gaggcagcgg cgctcgccga cgggccggcc gaggcgatgg
    19981 ctgccgaggg cgagggtgac ggagcgaccg acggcggcac gagcgtcggc gccggcgtag
    20041 ggcgcaatcc gaccggatcc ggcccggcga agacgaacgc ggccaggatc cccgcggcga
    20101 cgaggacgcc gacgaggagg ccggcgagga tcgcgaacgc cgtcttcatc gcggccgagg
    20161 gtaccagtac ggacgggcga gggcggcgcc gtggtgccgc tgctaggctg gccatcccgt
    20221 gaggaccctc ttcggcgcgc tccgcgatcg cctcctgccg gccgtcctga cggcggccgg
    20281 ggtgacgctg atcgccgccg ggctgctcag ctacaacggc ccggcctcgg ccgccgaccc
    20341 gtcgccgtcg gccgagccga cgatcgtctc ggccgacccg ggctcgagcc tcgcgttccc
    20401 gtcgctgccg ccgatcgacg cctcggcgcc gccatcgagc gggccgtccg ccagcccgaa
    20461 cgccaagcgc gtcgcgacgc gagtcgtcgt cgaggcgctc ggcatcgacc tgccgatcat
    20521 caagccgcgc ggcagctcat cgacctatcc acagtgcaac gtggcgctct acatccagga
    20581 gctgttccag cccggccagg gccgcgcgac ctacctctat gcccatgccc gcgacggcat
    20641 gttcggcccg atctacgaac gggcgatcca gaagaagagc ggcggtccga agtcgatgat
    20701 cgggatgatc gtccagatct acacgagcga cgacctgctc tacgagtacg cggtcaccga
    20761 ggtccgcctc caccagctga ccctcgacga tgcgatccac gccacgaccg aggagctctg
    20821 gctccagacc tccgaaggcc cgaaaggcac acccggcaag acgcagttgc tggcgaagcc
    20881 gctgatcacc ctgccggccg accacaaggc cgcccacccg acgccgcatc cggtgacctg
    20941 cgcctgacgg tcggggttcc gcgccctcag atcgagcgtc ggcggcgcat cgggcgaggc
    21001 gctgctagga tgcccgcccg atgcgcttcc ccgtccgcgc cctccgcagc cggctcctgc
    21061 cggccctcct gacggccgcc ggcgtgacgc tgctggccgc cggcctgctc cactacggcg
    21121 gcccggccga cgcccgaccg gcgggcgcga gcccgtcacc gtcgaacgtg gcggtccttc
    21181 cgacgccagg gttcatcgtc ccgagcctgc cgccgatcga cggctccccg cgaccgagcg
    21241 tggcgccgag ccccgccgcg aatcgcgtcg ccacccgcgt cgtcatcgat gagctcggga
    21301 tcgacctgcc gatcatcgcc cagccgaacc cgcgctatcc gtcgtgcaac gtcgcgatgt
    21361 actaccagga tccgcgactc ggccagccgg gcagcggccg ctcgatctac ctctacgccc
    21421 acgcccggac cggaatgttc gggccgctct acgaacgggc gatcctcggc cgcggcggcg
    21481 gccgcaagtc gctgatcggc ctgccggtcg aggtctacac gagcgacgac cagcgctacg
    21541 tctatacgat caccggcgtc cggccgaacg tgaaggctga cagccatttc ctcgacgggc
    21601 cgctcgcggt caaggccgag accctctggc tccagacctc gaccggcccg aacgccagct
    21661 acccgaagct gcaggtcgtc gcccagccac tcggcgtggt cccggcgagc cacgccgagt
    21721 ggctgggcga
//
LOCUS       DATAIW010000076        20535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_33051, whole genome shotgun sequence.
ACCESSION   DATAIW010000076 DATAIW010000000
VERSION     DATAIW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 20535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20535
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33051"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VHM48_02525"
     CDS             complement(<1..953)
                     /locus_tag="VHM48_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2754306.1"
                     /translation="MTGPNPTRPRASGQTTTIAAIAEQAGVSVPTVSKVINGRSDVSA
                     ATRRRVEAIILEHGYRTSDRSSRATPILELIFHELESEWALEIVRGVERVAGQHHLAV
                     VLSEMQGRRTPGRGWIESVLARRPTGVIAVFSDLSETLRDQLAARRIPFVVVDPTGEP
                     LHDTPSVGATNWNGGLTATRHLLALGHTRIGVIGGPEWILCSRARLDGYRAAMDEAGV
                     PIDPRLVSHGRFHVEEGIETGRALLALPDRPTAIFAGNDLQALGVYQAAREARLHIPE
                     DLSVVGFDDLPVAQWVGPPLTTIRQPLIDMAMVAAEMVLTIA"
     gene            1434..2762
                     /locus_tag="VHM48_02530"
     CDS             1434..2762
                     /locus_tag="VHM48_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2754307.1"
                     /translation="MGSRSRLAVLVATASIVVAACSNGNTATSAPSTAPASVAPASAP
                     APSTAAAPVTIEWWHITTGEPGKTTFQSIADAYTKAHPNVKINITVLENEAFKTKLAT
                     SIQSGQVPDLFQSWGGGIMAAQADASALKDITADIASWKDTINPGALSIYAYKGVQYG
                     VPWDMGMIGVWYNKKLFTQAGISAPPATWDEYLTDVGKLKAADIVPLAIAGKDEWPSM
                     HLWTYLTLRIGGGDNLAQMVQTGNWNTDACTKAGEQVLALNKLDPYQPGYKAADYNAE
                     AAAVGNRKAAMEVMGQWAPSVQNDQSADKKGLGSDLGWFPFPTVTGGAGAATDGVGGG
                     NGIAVGKDASPEAIDFLKFFNSVENATKLNSDNIGLSPVVGTESAIKDPNLQAVLAGR
                     GKATFMQLYLDQATSPAMGTAINDATIALFEGASDPAKVCKAITDAAAAQ"
     gene            2911..3789
                     /locus_tag="VHM48_02535"
     CDS             2911..3789
                     /locus_tag="VHM48_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2754308.1"
                     /translation="MTIALFLVPALTLYLVFVLVPIVQAAHFSLYKWNGLQPLTDFVG
                     LKNYQTAIASDTFRTAVANNLLIVGLSLAIQIPFSLGLAVLLNRRFPGRAIFRLIFFL
                     PYVLSEAITGIVFSLLLKPGSFVDSGLRSAGLGGLVQDWLGDSTIVMITLFAIISWKY
                     FGFHMILLLAGLQGIPHEIEEAALIDGADRRQAFRHVTLPLLGPTLRVSVFLSIIGAL
                     QLFDMVWVMTAGGPLSASNTMAIAMFKAGFKNTQMGFGSALAVILFIFGLIVALAYQR
                     FVLRRDVEGAVTVYNG"
     gene            3856..4716
                     /locus_tag="VHM48_02540"
     CDS             3856..4716
                     /locus_tag="VHM48_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2754309.1"
                     /translation="MKGPRAAFARPSPAGLAAYVILVVVAAAILVPIAYAILGGFKTN
                     GQLVSDPVAFLPSQWVFSNYTDVLFGKNAGAFWREAANSLIIAAIAVTVTVTFASLAA
                     FVFARMVFRGREATYTLFVFGLLFPSAVAILPLYILIRQLGLAGNLLGVALPQAAFAL
                     PLTIVILRPFFRSIPTELEDAARIDGCSSFGFFWRVLLPLARPALATVSVLAVVTTWN
                     AFLLPLILLNGADQWTLPLGVMNFSTEYSSDEARVLAFTVVAIIPAIIFYVAAERQIV
                     GGLTAGAVKG"
     gene            4716..7079
                     /locus_tag="VHM48_02545"
     CDS             4716..7079
                     /locus_tag="VHM48_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2754310.1"
                     /translation="MTTTEARYRDPSLPIDTRVADLLVRMTLEEKLAQLGSIWSFELF
                     SSETELDPARVRKRLATGIGQISRVAGATNLGAADAADAGNALQRFLVEETRLGIPAI
                     LHEETLHGLLAREATSFQQSIGAAATFDPELVEAIAASIRRRMLAIGARMALAPVLDL
                     ARDPRWGRIEETYGEDPYLASVLGCAYVRGIQGPDLSTGIAAVAKHLVGHGLAEGGLN
                     QAPAHIGSRELRDEQLLPFEAAVRTAGLAGVMPAYCDVDGLPCHASHELLTTILREEW
                     GFDGVVASDYTAVQMLVGEHRLTADLGTAAAMALGAGMDSELPTTAAYGEPLREALAD
                     GRIDIDVVDVAVGRTLRLKFRLGLFERPYVELPTTAALAAFEADERTLARDLARRSLV
                     LVENRGVLPLASDLARIAVVGPIADSARDLLGDYAHLPHIETLAELRHRANPFGFPSS
                     EVINPVDELSGHPTVLRAIQERFGARRVVHARGSGLLDGSDAEIAEAVAAARAADVAI
                     VVLGERSGLTEDATTGESRDRRDLGLLGRQQELLEAVVATGTPTVLVVVSGRPLAIPW
                     AAEQCAAVLLAWVPGEAGPAAIADVLAGDVDPGGRLPVTIPRHVGQVPLTYRHHPTGG
                     QSHWKVDYVDGPTSPLWPFGHGRSYTTFAIDHLRIDRTVLDTSGDVATIRVDVTNTGS
                     RPGDEIVQLYLRDEEATVARPILELRGFRRIHLDPGECRTVAFRVSTEQVAHIDANHR
                     RVVEPGAVGVHLGRSSADLALHGELTLIGPVVELVERTEYVTAASVE"
     gene            complement(7107..8309)
                     /locus_tag="VHM48_02550"
     CDS             complement(7107..8309)
                     /locus_tag="VHM48_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2754311.1"
                     /translation="MQAHHVTMLGTGLIGDFYTATLHGQRGRDHVRVVFSRSEERGAA
                     FANRWSIPEHTTDMKAGIEHDDTDTVVIGLPNYLHEEAVSLAAAAGKAVLCTKPLGRT
                     AEEARRMLEVVEKAGVFGGYLEDLCYTPKTLKAVKSVEGGAIGDVTWVRSRETHPGPH
                     SAWFWDGRLTGGGAIIDLGCHCIEIIRTFVGKGNRPVEVMCTTDTLVHPIDDEDNAIA
                     LVRFESGAIGQFEVSWTFRGGMDLRDEVAGTHGTIWLNHFLRTGFEMFTAGGGAGYVA
                     EKAETAAGWLFPVGDEVSELGYIDMFSDMFDAMEAGRPPRETLYDGYVVNAVMDACYR
                     SAKSRSWEPVELFEWRGGTTARIAKTPETYEGQTVIKRELLPDGRTKLILKDPATGDF
                     TDRIVAGG"
     gene            complement(8323..9381)
                     /locus_tag="VHM48_02555"
     CDS             complement(8323..9381)
                     /locus_tag="VHM48_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX2754312.1"
                     /translation="MSQAPLLTSVVGSHAHPGWFAHGIAAAVRGEYGPADLAELLDDG
                     VDLAIRDQERAGIDVISDGEMRRAGFFTAEFYRHLTGVRPLPPDRRLGAGAHDGLHRF
                     AVDDPISAPDGLGVVAEYTAARIRTERPLKVTLPGPYTLSGRLAYGDGQVYATRVAAA
                     EAFVPIVAAELRGLIDAGATIIQVDEPSPAIHPDAPADFVALFNAAIEPAVGRARLGA
                     HLCFGNFLGRPLAPRSYRPILDAMLAFAVDELVLEFANREMSEIGLLREISDAGRDVA
                     AGVIDVKSSHVETGAEVAARIEAILATGVPPERLALVPDCGFSQTARRLAVAKLHALV
                     AGRDIVRSGSASAATTHD"
     gene            complement(9378..10340)
                     /locus_tag="VHM48_02560"
     CDS             complement(9378..10340)
                     /locus_tag="VHM48_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754313.1"
                     /translation="MTFVRTVLGDIEPRDLGITYAHEHLIIDGGRPVELSPDFLLADV
                     DRMAAELDDAAAAGLRTAIDAMPADCGRNPAKLAELSLRSGVQIVAATGLHHEKFTGP
                     SHWSLRAPEDELTDLFAADIEIGIDERDYTGPIVHRTGIRAGIIKIGGSEGGPSARDL
                     RIFRAAAAAHARTGAPLHTHCEGGTGALEQIRVLVDAGVAAERISLSHVDKVVDRGYH
                     RELFASGAFAVYDQAFRWGSGSNGTLRLLEWAVADGHADQVMLGMDAARQRYYRAFGG
                     SPGLPYLLHEFSAAMDERGLDAALRNGFFVDNPARAFVFAEGGR"
     gene            complement(10337..11695)
                     /locus_tag="VHM48_02565"
     CDS             complement(10337..11695)
                     /locus_tag="VHM48_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754314.1"
                     /translation="MSRAWIADLPVIADADVCVVGSGSAGATAAIAAARGGASVVLVE
                     KLPFLGGTSTAVLDTIYGFWTPGDASRKVVGGIADEVVAGLRGLGPVVERPNTYGAGT
                     GITYLAEHLKVVWEQLVIAAGVRILLHAMLQDVDVDGGGRVRSIVVATKAGLRRVTAR
                     VFVDASGDADLCAFAGFGFETAGEFDPAQTLTTTFRMANVDRDRRRVLRTDELHALMT
                     AAAADGYDLPRRDGSDHVTPIDGVTATIMTRLEPNERRPDDGGPVNTTDPDFLTAAEI
                     AGRRQALEYARFLVDRVPGYEAASLVALGTQIGIRETRRVFGDARLTRDDVVAARQFD
                     DQIGLCGAPIEDHRAGPDTRWAYLPPGSAVGIPYRTLVVRDAVNVLVAGRCFSATHDA
                     HASVRSMAQCMAMGQAAGTAAAMAAAASPEGAVRSVVAAELRARLAADGAVLDLALEP
                     VA"
     gene            complement(11692..12495)
                     /locus_tag="VHM48_02570"
     CDS             complement(11692..12495)
                     /locus_tag="VHM48_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2754315.1"
                     /translation="MNRLAGRVCLVTGSTGIAAASAERFAAEGADVFVVSRTADHART
                     LAERIQADGGTAGWIAADLTDETAVDAAIEACVGRFGRIDGLFSVAGGSGRRFGDGPI
                     HTVGREAWDRTLELNLTTQALVCRSVVGRMRVQEPNESGTRGSILLMGSVTATNPAPE
                     FFATHAYAAAKGAINALMMTMAAAYLTDRIRVNVVAPGLTMTPMARRAAGDLAIRAYG
                     VRKQPLAGEMMDPDEVAHAAVFLLSDESRAITGQLLKVDGGWSVASVSP"
     gene            complement(12492..13463)
                     /locus_tag="VHM48_02575"
     CDS             complement(12492..13463)
                     /locus_tag="VHM48_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX2754316.1"
                     /translation="MTTERAAAAADGPAPTAELAGQVARLFFERQLTKVEIADRLGIS
                     RFRVARLLDQALADGIVRIEFRDAPGQDRDLGRRVMSRYGIGECLVAAADGDDLAVAR
                     LGADVVDGLVGRGDAIGVAWGTTVARVVEAMPARDDATIDVVQLAGSSTSLDAGNDPG
                     DLTRVLAERLGGRHHRIHAPAFVESADLRAALIRQPEVAATVGRFDKLAVALLGIGAF
                     GTGAAAPSSSLLGSGSLGADEVRRLAGLGAVGDLLVHPFAADGAFVATDLAGRAIAIS
                     IDQLRRVPHVVAVAAGPAKIVAIRGALLSGVVGSLVTDAATARGLLA"
     gene            complement(13460..14095)
                     /locus_tag="VHM48_02580"
     CDS             complement(13460..14095)
                     /locus_tag="VHM48_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX2754317.1"
                     /translation="MPVDSLLLRSSAVAVAIRNHRLIAVLRRIAPRERLLQLVDELAA
                     DGVRIFEITFDAVDAAADLRAVRAHLGETGWVGAGTIRTTDQLRAAIGAGAVFGVSPV
                     LDPAVLDAALAAGLPFVPGAYSPTEIDGAWRAGATFVKLFPGSSLGPSHIRELRGPLP
                     EVETIVTGGVDASNAVAFLAAGAVAVGIGSAIGRLTSDQRRTLVADIGAIR"
     gene            14726..15967
                     /gene="rho"
                     /locus_tag="VHM48_02585"
     CDS             14726..15967
                     /gene="rho"
                     /locus_tag="VHM48_02585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX2754318.1"
                     /translation="MREMSVPELRAVSQDLELETGTQERKDDLVERILERQTERAGHA
                     YASGILDIVDEGFGFMRRHGLLPSPDDVYVSSSQVRRFGLRIGDRVSGAVRAPREAEK
                     YWGMLRVDAVNGVDSDTARRRPVFGDLTPIFPLELINLENDPKNLSQRLINIVNPLGK
                     GQRALIVSPPKAGKTMLLKHIANGISTNYPDILLMVALIGERPEEVTDMRRGVKGEVI
                     SSTFDEPTENHTHVAEMALEIAKRQVETGRDVVILLDSITRLARAYNLALPPSGRTLS
                     GGIDPIALYPPKRFFGAARNIEEGGSLTVIATCLVDTGSRMDDVIYEEFKGTGNSELI
                     LDRKLAEKRIFPAIDVQRSGTRREELLLEEPVLKMVWTMRRMLSAVGTNEGIELLLNR
                     LAKTDNNVQFLGSLTKSLEAS"
     gene            16446..17606
                     /locus_tag="VHM48_02590"
     CDS             16446..17606
                     /locus_tag="VHM48_02590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2754319.1"
                     /translation="MSRPRLSGERLVTVTAVGLVAVALVLSNIGTGMASGSTGNTNGP
                     GNEPRIALGGAVSGLDNTAQGGIDDRIASEGAVTGLAGADLTDGSLGLVADDQAAIDG
                     PFSSDGMLLKPVSVDTTVADGADLLRTYRVRSGDTLTGIASKFHVSMMTVWWANELKN
                     KNDLKIGQTLTIPPVNGLVVTVRTSDTLETLAAKYHADPADIVAVNELADPVLVVGQT
                     LTIPGARSAQIPTPKPTKAPTKKPVVHSSGGGNSGSTRPPASYSGGKFAWPVAGGYIS
                     QYFHYGHYAIDIAADQGTRVTAAASGSVIFAGWKNNGGGYQVWIAHGSGLYTTYNHMS
                     SISVGRGQSVGRGQQVGRVGMTGNATGPHLHFEVWRGMIWDGGVRVNPLIYL"
     gene            17770..19080
                     /gene="obgE"
                     /locus_tag="VHM48_02595"
     CDS             17770..19080
                     /gene="obgE"
                     /locus_tag="VHM48_02595"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX2754320.1"
                     /translation="MFLDRVKIFVKAGAGGDGAATFRKEAHVPRGGPDGGDGGRGGSI
                     HVQVDAGQTTLRDFRFKHHFKATSGGRGSGSRRHGAAGDELFLDVPPGTAIFDDETGE
                     LLADLVAVGQVAMIARGGRGGLGNTHFKTSTHQAPKHAQKGEPGEERWLRLELRLIAD
                     IGLVGLPNAGKSTLLAALTAATPKIADYPFTTLEPNLGVMDLAGPDDPAGDERRPSIA
                     DVPGLIEGASLGAGLGHAFLRHVERTRILIHMVDGSGRDPEWDHDVIREELRAHDPAL
                     LEKPLLVVFNKLDQPTAVEAWPAFRAARLAAGQDVVAISATDGTGVDELRDRIADLLP
                     GADERDLPPEPAGVVVHRIEAMGDGFSVEQLEDGVFRVRGKRIERVAAQTNFEVEESA
                     ERFQRDLARLGIDDELRRAGVTAGDVVRIGATELTWEAQPWEER"
     gene            19077..20216
                     /gene="nadD"
                     /locus_tag="VHM48_02600"
     CDS             19077..20216
                     /gene="nadD"
                     /locus_tag="VHM48_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEX2754321.1"
                     /translation="MTAAPGPVQVRPGSIGILGGTFDPFHAGHLALARAARDQLGLER
                     ILVVPAAVPPHKRDRPISPAAVRLALVEAGIAGEPRLEASRIELDRDGPSWTVVTVAD
                     LAAAERAAGREPDLTVILSVESFAGLPTWHEPLRVLDLARIAVAPRAGHEPPDPAWIA
                     ANFPGREDRILVIDGPRVDVSASEVRRRAATGESLAGLVPPGVAKAIAADRLYRHPEP
                     QEDESIVTDSAPLAGTPADQPSATAHIPRPDGLPSRDAAAAVVPAAERPSLDIARRIV
                     ELAEDKKAADIILLDLTGLTTLADAFVICSGGSERQLDAIADGVIEGMRSEKVRPIGR
                     EGTAASHWVLVDFGAVVVHIFTPPEREYYSLEKHWAEARTVLRVQ"
BASE COUNT         3073 a         7367 c         7105 g         2990 t
ORIGIN      
        1 gcgatggtga ggaccatctc ggccgcgacc attgccatgt cgatcaacgg ctgacggatc
       61 gtcgtcagcg gcggtcccac ccattgggca actggcaggt catcgaagcc gaccacgctg
      121 agatcctcgg ggatgtggag ccgggcttcg cgcgcggcct ggtagacgcc gagcgcctgg
      181 aggtcattgc cggcgaagat cgccgtcggg cggtccggga gcgcgagcaa cgcccggccg
      241 gtctcgatcc cttcctcgac atggaagcgc ccgtggctga cgagccgcgg atcgatcggg
      301 acaccggcct cgtccatcgc ggcccgatac ccgtccagcc gggcgcgact gcagaggatc
      361 cattccggtc cgccaatcac cccgatccgc gtgtggccga gggcgaggag gtgacgtgtc
      421 gccgtcaggc cgccgttcca gttcgtcgcg ccgaccgagg gcgtgtcgtg gagcggctcg
      481 cctgtcgggt cgacgacgac gaacgggatc cgccgggcag ccagctgatc ccggagggtc
      541 tcactcaggt ccgagaagac ggcgatgacc ccggtcggcc gacgagcgag gacgctctcg
      601 atccagccgc ggcccggcgt ccgtcggccc tgcatctccg acagaacgac cgcgaggtga
      661 tgctggcccg cgacgcgctc gacgccccgg acgatctcca gcgcccactc gctttcgagc
      721 tcgtggaaga tcagctccag gatcggcgtc gcccgactcg atcggtccga cgtccgatag
      781 ccatgctcga ggatgatggc ctcgacccgc cggcgggtcg ccgcggagac gtcggaccgt
      841 ccgttgatca ccttggagac ggtcggaacg gacacccccg cctgctcggc gatcgcggcg
      901 atcgtcgtcg tctgaccgga ggctcgtggt cgggtcgggt tcgggccggt cacgggcgca
      961 gtgtaacgaa ctggccgccg cttcgaaagt ttcgaatgcg ctcccggggt cgtcgccctc
     1021 gaacggtgat caagatcctg cctggcagga gcacgaaccg gtccccgggc attgacagca
     1081 atcgacccac atgactacag tgcccgagcc gaaactatcg accttcagtg cgcaactttc
     1141 gcggcagagg aggctgcgaa gcgcagcatc gtcgagaggt cgtcggcggc tggtcaagga
     1201 tggccgatca acggatcccg tctcgccaga ctcctcctcc ctggcggggc gggatcgacg
     1261 cccgcgccgc ccggaacggc gtcgcggctg tcacgcggaa gattgcgaat cgggtcgcgg
     1321 cgccgcgatc ggatcgacca agcggacgaa tgatcgcaga ggaggaaacc gacatcgccg
     1381 accggcccac ccgaacgcgg tgactcgtcc agccaagaga ggaggccagg ccaatgggat
     1441 ctcgttcacg gctcgcggta ctcgtcgcga ccgcgtcgat cgtcgtcgca gcctgttcca
     1501 acggaaacac ggccaccagt gcgccgtcca ccgccccggc atccgtcgcg ccggcatcgg
     1561 cgccggcgcc gtcaacggca gccgcaccgg tcacgatcga gtggtggcac atcacgaccg
     1621 gcgaacccgg caagaccacg ttccagtcga tcgccgacgc ctacacgaag gcccacccga
     1681 acgtcaagat caacatcacg gtcctcgaga acgaggcgtt caagacgaag ctcgcgacat
     1741 cgatccagtc cggccaggtg ccggacctct tccagtcgtg gggcggcggc atcatggccg
     1801 cccaggcgga cgccagtgcg ctcaaggaca tcactgctga catcgcgtcc tggaaggaca
     1861 cgatcaaccc cggtgccctc agcatctacg cctacaaggg cgtccagtac ggcgtcccgt
     1921 gggacatggg catgatcggg gtctggtaca acaagaagct gttcacccag gcaggcatct
     1981 cggctccgcc cgccacctgg gacgaatatc tcaccgacgt cggcaagctc aaggcggccg
     2041 acatcgtgcc gctggcgatc gccggcaagg acgagtggcc gtccatgcac ctgtggacgt
     2101 acctgaccct gcggatcggc ggtggcgaca acctcgccca gatggtccag acgggcaact
     2161 ggaacacgga cgcctgcacg aaggccggtg agcaggtcct cgccctcaac aagctcgatc
     2221 cctatcagcc cggctacaag gcggccgact acaacgccga ggccgccgcg gtgggcaacc
     2281 gcaaggccgc aatggaggtc atgggccagt gggcgccctc ggtccagaac gaccagagtg
     2341 ccgacaagaa aggcctcggc agtgatctcg gctggttccc gttcccgacc gtgaccggtg
     2401 gtgcgggtgc ggcgaccgac ggggtcggcg gtggcaacgg catcgccgtc ggcaaggacg
     2461 cctcgcccga ggcgatcgac ttcctgaagt tcttcaacag cgtcgagaac gcgaccaagc
     2521 tcaattccga caacatcggc ctgtcgccgg tcgtcggcac ggagagcgcg atcaaggatc
     2581 cgaacctgca ggccgtcctc gccggtcgcg gcaaggcgac gttcatgcag ctctatctcg
     2641 accaggcgac ctccccggcc atggggaccg ccatcaacga cgcgacgatc gccctcttcg
     2701 agggtgcgtc ggatccggcc aaggtctgca aggcgatcac cgatgccgcg gcggcccagt
     2761 aggcacgact gcggtccgga tcacccgtag actcgtgaac ccgagcgggc cggctcagcc
     2821 ggcccgctcg tttccagctc gcgagcagag gacgcgatga gggcatacgg aggcgggtcg
     2881 cggctcgcgg caagacggcc tgcaaagtgg gtgacgatcg ccctgttcct cgtgcctgcc
     2941 ctgacgctgt atctcgtctt cgtcctcgtc ccgatcgtcc aggctgccca tttcagcctc
     3001 tacaagtgga acggactgca gcccctgacc gacttcgtcg gcctgaagaa ctaccagacg
     3061 gccatcgcaa gcgacacctt caggacggcc gttgccaaca acctgctgat cgtcgggctc
     3121 tcgctcgcga tccagatccc gttctcgctc ggcctggccg tactcctcaa ccgccggttc
     3181 ccgggccggg cgatcttccg cctgatcttc ttccttccct acgtcctgtc cgaggcgatc
     3241 accggcatcg tcttctcgct gctcctcaag cccggatcgt tcgtcgactc ggggctgcgc
     3301 agcgcgggcc tcggcggcct ggtccaggac tggctcggcg actcgacgat cgtgatgatc
     3361 accctcttcg cgatcatctc gtggaagtac ttcggcttcc acatgatcct cctgctcgcc
     3421 ggcctccagg ggattcccca cgagatcgag gaggccgccc tgatcgacgg agccgatcgc
     3481 cggcaggcct tccgccacgt caccctgccg ctgctcggac cgaccctgcg cgtgtcggtg
     3541 ttcctctcga tcatcggggc cctccagctc ttcgacatgg tctgggtgat gaccgccggc
     3601 ggacccctga gcgcctcgaa cacgatggcg atcgcgatgt tcaaggccgg cttcaagaac
     3661 acccagatgg ggttcgggag cgcccttgcc gtgatcctgt tcatcttcgg cctcatcgtc
     3721 gccctcgcct atcagcgctt cgtgctgcgg cgcgacgtcg agggcgccgt gacggtctac
     3781 aacggctgat ggcgtggcca cgagcgcctc gtccacgacc cgcccgacgg ctcgcccgat
     3841 gaaacggctc gaccgatgaa ggggccgcgg gccgcgttcg cacgccccag cccggccggg
     3901 ctcgctgcct acgtgatcct ggtcgtcgtg gcggcggcca tcctggtccc gatcgcctac
     3961 gcgatcctcg gaggcttcaa gaccaacggt cagctcgtca gcgacccggt ggcgttcctg
     4021 cccagccagt gggtcttctc gaactacacc gatgtcctgt tcggcaagaa cgccggcgca
     4081 ttctggcgcg aggcggcgaa cagcctgatc atcgcggcga tcgcggtcac cgtgacggtc
     4141 acgttcgcct cgctcgccgc cttcgtcttc gcccggatgg tcttccgcgg ccgggaagcg
     4201 acgtacaccc tcttcgtctt cgggctgctg ttcccgtcgg cggtggcgat cctcccgctg
     4261 tacatcctca tccgccagct cggcctggcc gggaacctgc tcggcgtcgc cttgccacag
     4321 gccgccttcg ccctgccctt gacgatcgtc atcctgcggc cgttcttccg aagcatcccg
     4381 accgagctcg aggacgccgc ccggatcgat ggctgcagct cgttcggttt cttctggcgg
     4441 gtcctcctgc cgctggcccg acccgccctc gcgaccgtca gcgtcctcgc ggtcgtcacg
     4501 acctggaacg cgttcctcct gcccttgatc ctgttgaacg gcgcggacca atggacgctg
     4561 ccgctgggag tgatgaactt ctcgaccgaa tacagctccg acgaggcccg ggtcctcgcg
     4621 ttcacggtcg tcgcgatcat cccggcgatc atcttctatg tcgccgcaga gcggcagatc
     4681 gtcggcggcc tgacagccgg cgcggtgaag ggctgatgac gacgaccgag gcgcgctacc
     4741 gcgatccttc gctcccgatc gacacccgcg tcgcggacct gctcgtccgg atgacgctcg
     4801 aggagaagct cgcccagctc ggctcgatct ggtcgttcga gctgttcagc agtgagacgg
     4861 agctcgatcc tgcgcgtgtt cgcaagcgtc tcgcgaccgg gatcgggcag atcagtcggg
     4921 tggcgggggc gaccaacctc ggcgccgccg acgctgccga cgccggcaac gcgctccagc
     4981 ggttcctcgt cgaggagacc cgcctgggca tcccggcgat cctccacgag gagacgctgc
     5041 acggcctgct tgcccgcgag gcgacctcct tccagcagtc gatcggggcg gcagcaacct
     5101 tcgatccaga gctcgtcgag gcgatcgcgg cctcgatccg ccgacgcatg ctcgcgatcg
     5161 gcgcgcggat ggccctcgcg ccggttctcg acctggcgag ggatccgcgc tggggtcgca
     5221 tcgaggagac ctatggcgag gatccctacc tcgcctcggt cctcgggtgt gcttatgtcc
     5281 gtggcatcca gggcccggat ctgtccaccg gcatcgcagc cgtcgcgaag catctggtcg
     5341 gtcacggtct ggccgagggc gggctcaacc aggcacccgc ccatatcggc tcccgtgagc
     5401 tgcgcgacga gcagctcttg ccgttcgagg cggccgtccg gacggcgggt ctggccggcg
     5461 tgatgcccgc ctattgcgac gtcgacggcc tgccgtgcca tgcctcgcac gagctgctga
     5521 cgacgatcct gcgcgaggaa tggggcttcg atggcgtcgt cgcgtccgac tacaccgccg
     5581 tccagatgct cgtcggcgag caccggctga ccgcggatct cgggacggcg gcggcgatgg
     5641 ccctcggtgc cggaatggac agcgagctgc cgacgaccgc ggcgtacggg gagccgctgc
     5701 gcgaggccct ggccgacggg cgcatcgaca tcgacgtcgt cgatgtcgcc gtcgggcgga
     5761 cgcttcggct gaaattccgg ctcggactgt tcgagcggcc atatgtcgag ctccccacga
     5821 ccgccgcgct ggccgccttc gaggccgacg agcggacact cgcacgcgac ctcgcccggc
     5881 gctcgctcgt cctcgtcgag aaccgcgggg tcctgccgct ggcatccgat ctcgcccgga
     5941 tcgcggtggt cgggccgatc gccgacagcg cccgcgacct gctcggcgac tacgcccacc
     6001 tcccgcacat cgagacgctg gccgagctcc gccatcgggc gaacccgttc gggttcccct
     6061 cgagcgaggt catcaacccg gtcgacgagc tatccgggca cccgaccgtc ctccgagcga
     6121 tccaggagcg gttcggcgct cggcgcgtcg tccacgcccg tggctcgggc ctgctcgacg
     6181 ggtccgacgc ggagatcgcg gaggccgtcg cggcggcccg cgccgccgat gtcgcgatcg
     6241 tcgtcctcgg cgagcgatcc gggctgaccg aggacgcgac gaccggcgag tcccgcgatc
     6301 ggcgcgacct cgggttgctc ggccgccagc aggagctgct ggaggccgtc gtcgcgaccg
     6361 gcacgccgac cgtcctcgtc gtggtgagcg ggcgtccgct ggcgatcccg tgggcggccg
     6421 agcagtgcgc ggccgtgctc ctggcgtggg tcccgggtga ggcaggcccg gcggcgatcg
     6481 ccgacgtgct ggcgggcgat gtcgacccgg gtgggcggtt gccggtcacg atcccgcgcc
     6541 atgtcggcca ggtgcccctg acctatcgcc accacccgac cggcggtcag tcgcactgga
     6601 aggtcgatta cgtcgacggc ccgacgtccc cgctgtggcc gttcggccac ggtcgttcct
     6661 acacgacctt cgcgatcgac catctgcgga tcgaccggac cgtgctcgac acgagcggcg
     6721 acgtcgcgac gatccgggtc gatgtcacga acaccgggtc gcgtccgggc gacgagatcg
     6781 tccagctcta cctccgcgac gaggaggcga cggttgcccg gccgattctc gagctgcgcg
     6841 gcttccggcg gatccatctt gacccgggcg agtgccggac agtcgccttc cgcgtctcga
     6901 ccgagcaggt cgcgcatatc gacgcgaacc atcggcgggt cgtcgagccg ggcgcggtcg
     6961 gcgtccacct cgggcgctcg tcggccgacc tggcgctcca tggggagctc accctgatcg
     7021 ggccggtggt cgagctcgtg gagcgaaccg agtacgtgac ggccgccagc gtcgagtgat
     7081 cgggcggcca cccgcccccg tcatcctcag ccgccggcga cgatccggtc ggtgaagtcg
     7141 ccggtcgcgg ggtccttgag gatcagcttc gtccggccgt ccggcaggag ctcgcgcttg
     7201 atcacggtct ggccctcgta cgtctcgggc gtcttcgcga tccgggcggt cgtcccgccg
     7261 cgccactcga acagctcgac cggctcccat gagcggctct tcgccgaccg gtagcaggcg
     7321 tccatgaccg cattcacgac atagccgtcg tagagcgtct cgcgcggcgg ccggccggcc
     7381 tccatcgcgt cgaacatgtc cgagaacatg tcgatgtagc cgagctcgga cacctcgtcg
     7441 ccgaccggga acagccagcc ggccgcggtc tcggccttct ccgcgacgta gcccgcgccg
     7501 ccgcctgcgg tgaacatctc gaacccggtc cgcaggaagt ggttcagcca gatcgtcccg
     7561 tgggtcccgg cgacctcgtc gcggaggtcc atcccgccgc ggaacgtcca gctcacctcg
     7621 aactggccga tcgcaccgga ctcgaaccgg acgagggcga tcgcgttgtc ctcgtcgtcg
     7681 atcgggtgga cgagggtgtc ggtcgtgcac atcacctcga ccggccggtt gcccttgccg
     7741 acgaaggtcc ggatgatctc gatgcagtgg cagccgaggt cgatgatcgc gccgccgccg
     7801 gtcagccggc cgtcccagaa ccaggcgctg tgcggccccg gatgggtctc gcgggaacgg
     7861 acccaggtca cgtcgccgat cgcgccaccc tcgaccgact tgaccgcctt gagggtcttc
     7921 ggggtatagc agaggtcctc gagatagccg ccgaagacgc ccgccttctc gacgacctcg
     7981 agcatccggc gagcctcctc ggccgtccgc ccgagtggct tcgtgcagag gacggccttg
     8041 cccgcggcgg ccgccagcga gacggcctcc tcgtggaggt agttcggtag gccgatgacg
     8101 acggtgtcgg tgtcgtcgtg ctcgatcccc gccttcatgt cggtcgtgtg ctccgggatc
     8161 gaccaacggt tggcgaacgc cgccccgcgc tcctcggacc gcgagaacac gactcggacg
     8221 tggtcgcggc cgcgttggcc gtggagggtc gcggtgtaga agtcgccgat gagcccggtg
     8281 ccgagcatcg tgacgtggtg ggcttgcatc gagcgtctcc tctcagtcgt gggtggtggc
     8341 ggcggaggcg gaacctgacc ggacgatgtc gcggccggcg acgagggcgt ggagcttggc
     8401 gacggcgagg cggcgggcgg tctggctgaa gccgcaatcg gggacgaggg cgaggcgctc
     8461 cggcgggacg ccggtcgcga ggatggcctc gatccgggcg gcgacctcgg cgcctgtctc
     8521 gacatgggag ctcttgacgt cgatcacccc tgcggcgacg tcccggccgg catcgctgat
     8581 ctcgcgcagg agcccgatct cgctcatctc gcgattggcg aactcgagga cgagctcgtc
     8641 gaccgcgaag gcgagcatcg cgtccaggat cggccggtag gagcggggcg cgagcggccg
     8701 gccgaggaag tttccgaagc agagatgggc accgagccgg gctcgcccga cggctggctc
     8761 gatcgcggcg ttgaacaggg cgacgaagtc cgcgggcgcg tccgggtgga tcgccggcga
     8821 gggctcgtcg acctggatga tcgtggcccc ggcgtcgatc agcccgcgga gctccgccgc
     8881 gacgatcggg acgaaggcct cggcggcggc gacccgggtc gcgtagacct ggccgtcgcc
     8941 gtacgcgagg cggccggaca gcgtgtatgg cccgggcagg gtcaccttga gcgggcgctc
     9001 cgtccggatg cgcgccgcgg tgtactcggc gaccacgccg agcccgtccg gcgcggagat
     9061 cggatcgtcc acggcgaagc gatgcagccc atcgtgggcg ccggcgccga gacgccggtc
     9121 gggcggcagg ggccggacgc cggtcaggtg gcgatagaac tcagccgtga agaagcccgc
     9181 ccgacgcatc tcgccgtcgc tgatcacgtc gatcccggcc cgctcctggt cgcggatcgc
     9241 gaggtctacg ccgtcgtcga ggagctccgc gagatcggcc gggccgtact cgccgcgcac
     9301 ggccgccgcg atcccgtggg cgaaccagcc cgggtgcgcg tgcgacccga cgacgctggt
     9361 caggagcggt gcctggctca tcggcctccc tcggcgaaca cgaacgcccg ggctggattg
     9421 tcgacgaaga agccgttccg gagcgcggcg tccaggcctc gctcgtccat cgccgcgctg
     9481 aactcgtgga gcaggtaggg cagcccgggc gagccgccga aggcccggta gtagcgctgt
     9541 cgagcggcat ccatgccgag catgacctgg tcggcatggc cgtcggcgac cgcccactcg
     9601 aggagccgga gcgtgccgtt cgagccgctg ccccagcgga acgcctggtc gtagaccgcg
     9661 aacgcgccgc tggcgaacag ctcgcggtgg tagccgcggt cgacgacctt gtcgacgtgg
     9721 ctcagcgaga tccgctcggc ggcgaccccc gcgtcgacca ggacccggat ctgctcgagc
     9781 gcgccggtcc cgccctcgca gtgggtgtgg agcggggcgc cggtccgggc atgggcggcg
     9841 gccgcggccc ggaagatccg caggtcgcgt gccgagggac ctccctcgct gccgccgatc
     9901 ttgatgatcc cggcccggat tccggtccgg tggacgatcg gaccggtgta gtcgcgctcg
     9961 tcgatcccga tctcgatgtc ggccgcgaac aggtcggtca gctcgtcctc cggggcgcgg
    10021 aggctccagt gcgacgggcc ggtgaacttc tcgtgatgga gcccggtcgc cgccacgatc
    10081 tggaccccgc tccggagcga gagctcggcc agcttggccg ggttgcggcc gcagtcggcc
    10141 ggcatcgcgt cgatggccgt ccgcaggccg gcggccgcgg catcgtccag ctcggccgcc
    10201 atccggtcga cgtcggcaag gaggaagtcg ggcgacagct cgaccggccg gccgccgtcg
    10261 atgatcaagt gctcgtgggc atacgtgatg cccaggtcgc gcggttcgat gtcgccgagg
    10321 accgtccgga cgaaggtcat gcgacaggct ccagggcgag atcgaggacc gcgccgtcgg
    10381 cggcgagacg ggcgcgcagc tcggcggcga cgaccgatcg gacggcgcct tccggcgacg
    10441 cggccgccgc catcgccgcc gcggtgcccg ctgcctggcc catcgccatg cactgggcca
    10501 tcgaccggac cgacgcatgg gcatcgtgag tggccgagaa gcagcgaccg gcgacgagga
    10561 cgttgacggc gtcgcggacg acgagggtcc ggtaggggat cccgaccgcg gagcccggcg
    10621 gcaggtacgc ccagcgcgtg tcggggcccg cccggtggtc ctcgatcgga gcgccacaca
    10681 gtccgatctg gtcgtcgaac tggcgggccg cgacgacgtc gtcgcgggtc aggcgggcgt
    10741 cgccgaagac gcgccgggtc tcgcggatgc cgatctgggt gccgagcgcg accagcgaag
    10801 cggcctcgta gcccggaacc cgatcgacga ggaagcgggc gtactcgagg gcctggcggc
    10861 gaccggcgat ctcggcggcg gtcaggaagt ccgggtcggt ggtgttgacg ggaccgccgt
    10921 cgtccggccg acgctcgttc ggctcgagac gggtcatgat cgtcgccgtc acgccgtcga
    10981 tcggggtgac gtgatccgag ccgtcgcggc gggggaggtc gtagccgtcg gctgctgccg
    11041 cggtcatgag cgcgtggagc tcgtcggtcc ggagcacccg gcgccggtcg cgatcgacgt
    11101 tcgccatccg gaaggtcgtg gtcagggtct gggcggggtc gaactcgccg gcggtctcga
    11161 agccgaaccc agcgaatgcg cacaggtcgg cgtcgccgct cgcgtcgacg aagacgcgcg
    11221 ctgttactcg gcgcaggccg gccttggtgg cgacgacgat cgagcggacc cgcccgccgc
    11281 cgtcgacgtc gacgtcctgg agcatcgcgt ggaggaggat gcgcacgccg gcggcgatga
    11341 cgagctgttc ccagacgacc ttcaggtgct cggcgagata ggtgatgccg gtcccggcgc
    11401 cgtaggtgtt cggtcgctcg acgaccggtc cgagcccgcg gagcccggca acgacctcgt
    11461 cggcgatccc gccgacgacc ttgcgcgacg cgtcgcccgg cgtccagaag ccgtagatgg
    11521 tgtcgaggac cgcggtgctc gtgccgccga ggaacggcag cttctcgacc agcaccaccg
    11581 aagccccgcc gcgggcagcc gcgatcgccg ccgtggcacc ggccgagccg gagccgacga
    11641 cgcagacatc agcgtcggcg atcaccggca ggtcggcgat ccaggccctg ctcacggaga
    11701 caccgaggcg accgaccagc cgccgtcgac cttcaggagc tggccggtga tcgcccgcga
    11761 ctcgtccgag agaaggaaca ccgccgcgtg ggcgacctcg tcggggtcca tcatctcgcc
    11821 cgcgagcggc tgcttgcgta cgccgtacgc gcggatcgcc aggtcccctg ccgcgcgtcg
    11881 cgccatgggt gtcatggtca gccccggcgc gacgacgttc acccggatcc ggtcggtgag
    11941 gtaggccgcc gccatcgtca tcatcagcgc gttgatcgca cccttcgcgg ccgcgtacgc
    12001 gtgcgtcgcg aagaactccg gcgcggggtt cgtggcggtg acgctgccca tcagcaggat
    12061 cgagccgcgc gtcccggact cgttcggttc ctggacccgc atccgcccga cgacgctccg
    12121 gcatacgagc gcctgggtcg tcaggttcag ctcgagggtc cggtcccagg cctcgcggcc
    12181 gaccgtgtgg atcggtccgt cgccgaaccg ccggccgctc ccgccggcga ccgagaacag
    12241 gccgtcgatc cgcccgaacc gcccgacgca cgcctcgatc gccgcgtcca cggcggtctc
    12301 gtcggtcagg tcggcggcga tccagccggc cgtgccccca tccgcctgga tccgttcggc
    12361 gagcgtgcgg gcatggtccg cggtccgcga cacgacgaag acgtccgcac cctcggcggc
    12421 gaaccgttcc gcggacgccg cggcgatccc cgtggagccg gtgacgaggc agacgcgacc
    12481 ggcgaggcga ttcacgcgag cagtcctcga gccgtcgccg catccgtgac caggctgccg
    12541 acgacgccac tcaggagcgc gccgcggatc gcaacgatct tggccggtcc ggcggcgaca
    12601 gcgacgacgt gtggcacccg gcgcagctgg tcgatcgaga tcgcgatcgc ccgcccggca
    12661 aggtccgttg ccacgaacgc gccgtcggca gcgaagggat ggacgagcag gtcgccgacg
    12721 gcgccgagcc cggccagccg gcggacctcg tccgcgccga gcgaaccgga gccgagcaac
    12781 gacgagctcg gtgcggcggc gccggtcccg aaggcgccga tgccgagcag ggcgacggcg
    12841 agcttgtcga atcgtccgac ggtggcggcg acctccggct ggcgaatgag ggcggcgcgg
    12901 aggtcggccg actcgacgaa ggccggcgcg tggatccgat ggtggcgacc gccgagccgc
    12961 tcggcgagga cccgcgtcag gtcgccggga tcgttgcccg cgtcgaggct ggtcgagctg
    13021 cccgccagct ggaccacgtc gatggtcgcg tcgtcgcggg cgggcatcgc ctcgacgacg
    13081 cgcgcgaccg tcgtacccca ggcgaccccg atcgcgtcgc cgcgaccgac gagcccgtcg
    13141 accacgtcgg cgccgagacg ggcgaccgcg agatcgtcgc cgtcggcagc ggccacgagg
    13201 cactcgccga tgccatagcg cgacatcacc cgtcggccga gatcccgatc ctgcccgggc
    13261 gcatcccgga actcgatccg gacgatgcca tcggcgaggg cctggtcgag caggcgcgca
    13321 acccggaagc gggagatccc gagccggtcc gcgatctcga ccttcgtgag ctggcgctcg
    13381 aagaacagcc gcgcgacctg gccggcgagc tcggcggtcg gcgccgggcc gtcggcggcc
    13441 gcggccgcgc gctcggtcgt catcggatcg caccgatgtc ggcgacgagc gtccgtcgct
    13501 ggtccgatgt cagtcgcccg atcgccgagc cgatcccgac cgcgacggcg ccggccgcca
    13561 ggaacgcgac cgcgttcgac gcgtcgacgc cgccggtgac gatggtctcg acttccggca
    13621 gagggccgcg cagctcgcgg atgtggctcg gcccgagcga cgagccgggg aacaacttca
    13681 cgaacgtcgc cccggcgcgc cacgcgccgt cgatctcggt cgggctgtag gcgccgggca
    13741 cgaacgggag acccgccgcg agggccgcgt cgagcacggc cggatcgagc acgggactga
    13801 cgccgaagac cgcgccggcg ccaatcgccg cacggagctg gtcggtcgtc cggatcgtgc
    13861 ccgcgccgac ccagccggtc tcgccgaggt gggcccggac cgcccgcagg tccgccgcgg
    13921 cgtcgaccgc gtcgaaggtg atctcgaaga ttcgaacgcc gtccgcggcc agctcgtcga
    13981 cgagttggag cagtcgctcc cgtggcgcga tccggcggag gaccgcgatg agccgatggt
    14041 tgcggatcgc gaccgcgacc gcgctcgagc gcagcaggag cgaatcgaca ggcaccgagc
    14101 gaggataggc ttgtgaccag atgagcgtca accgctcaga tgagcaagtt ccgcggaagg
    14161 cggccctcgc gatgtgcgtt caatgaacct atgcgacgtc ccggatagca gcggcgccac
    14221 gaacggtccg gattggcgca tcagatgcac gaagccgacc gattcctgcc gccccaggag
    14281 cgaatcggcg tccgtggtgg actgttcggt cgcgtaggtt catctggcgc acgtgaatcc
    14341 cggaaaggcg catcggatgc acgaaagcga cggcgagaga cggcatcggc ggcctggatc
    14401 cgggtcgaga cggatggacg tgccggacga gtccagcgca ttgacgccgc gtggatcgcc
    14461 gcgtatgatt cccggcatcc ccgaccggcc tgcgcagcgg agcgattccg cgcggcatcc
    14521 agcccggacg cggatctcga ctccctgccc acgactctcc tgagggaatt tcccacccat
    14581 gccccaactc ggtcccgacc agcgttcccg gaatcggcgg ccgcgccgtc gcaaccaggt
    14641 gtcgtcccgc gcccccgtca ccgagtacga ggagcagcag gagctcgagg catcacgcga
    14701 gcgaaacgac ctcgacgtct ccgacctgcg ggagatgagt gtccccgagc tccgcgcggt
    14761 cagccaggac ctggagctcg agaccggtac ccaggagcgc aaggacgacc tcgtcgagcg
    14821 gatcctcgag cgccagaccg agcgcgccgg ccacgcctac gcgagcggca tcctcgacat
    14881 cgtcgacgag ggcttcggct tcatgcgccg gcacggcctc ctgccgagcc cggacgacgt
    14941 ctacgtcagc tcgagccagg tccgccggtt cggcctgcgg atcggcgatc gcgtcagcgg
    15001 cgcggtccgc gccccgcgcg aggccgagaa gtactggggc atgctccggg tcgacgcggt
    15061 caacggcgtc gactccgaca ccgcccgtcg gcggcccgtc ttcggcgacc tgacgccgat
    15121 cttcccgctc gagctgatca acctcgagaa cgatccgaag aacctcagcc agcggctgat
    15181 caacatcgtc aacccgctcg gcaagggcca gcgcgcgctg atcgtcagcc cgccgaaggc
    15241 cggcaagacg atgctgctca agcacatcgc caacggcatc agcaccaact acccggacat
    15301 ccttctgatg gtcgccctga tcggcgagcg gcccgaggag gtcaccgaca tgcggcgggg
    15361 cgtcaagggc gaggtcatca gctcgacctt cgacgagccg accgagaacc acacccacgt
    15421 cgccgagatg gccctcgaga tcgccaagcg ccaggtcgag accggccgcg acgtcgtgat
    15481 cctgctcgac tcgatcaccc gtcttgcccg cgcctacaac ctcgccctgc cgccgtccgg
    15541 ccggacgctg tcgggcggca tcgacccgat cgccctctat cccccgaagc ggttcttcgg
    15601 cgccgcccgg aacatcgagg agggcgggtc gctgaccgtc atcgcgacct gtctcgtcga
    15661 caccggctcg cggatggatg acgtcatcta cgaggagttc aagggcaccg gcaactctga
    15721 gctcatcctc gaccggaagc tggccgagaa gcggatcttc cccgcgatcg acgtccagcg
    15781 atccggcacc aggcgcgagg agctcctgct cgaggagccc gtcctcaaga tggtctggac
    15841 gatgcgccgg atgctgtcgg cggtcgggac gaatgagggc atcgagctcc tcctcaaccg
    15901 gctcgcgaag accgacaaca atgtccagtt cctcggctcg ctgaccaaga gcctcgaagc
    15961 ctcttaggcc gtaccgctca catcggcggt cgcgcctgag cacgccatcg cggcgttgcg
    16021 ccctgcgctg acgcactcat gcgcggcgaa cgcagtccgg gtcgggtgtt ctggccgccc
    16081 ctcgcgatga acacagcacg ggcggcgcgt gcccggtgcg acagccgtgg tttgcgtggc
    16141 cgttaccagt ctgttacatt ttgcccgaag tcgacagctc gctgcatgcg ggctcggccg
    16201 cggacagaga cccccagctc gaagacgcga ccctctcgga cggttctgac caagcgccca
    16261 tcccacgcgc ttccatcccc gcgttgaccg tccggtttca ccaggagacc gtattgcgca
    16321 acgcaacggt cggtgtgcag cgcctcattg cgcgcctgcg acccgacccg ggccaccgcc
    16381 cagcggcggc cgtcacattc cagcgacgca gcatttcgat cgcgcgcagc ctccgcgacg
    16441 gatcgatgag ccgtccccgc ctgtcgggtg agcgcctcgt caccgtcacg gccgtcgggc
    16501 tcgtcgccgt cgcgctcgtg ctcagcaaca tcggcacggg catggcgtct ggctcgaccg
    16561 gcaacacgaa cggccccgga aacgaacccc ggatcgccct cggcggtgcg gtgtcgggcc
    16621 tcgacaacac cgcccagggc gggatcgacg accggatcgc gagcgaaggc gccgtcaccg
    16681 ggctcgccgg cgccgacctg acggatggct ccttgggcct cgtggccgac gaccaggccg
    16741 cgatcgacgg gccgttcagc tccgacggga tgctcctgaa gcctgtctcg gtcgacacca
    16801 ccgtcgccga cggtgcggat ctcctgcgca cgtatcgggt ccgctccggc gacaccctga
    16861 ccggcattgc cagcaagttc cacgtgtcga tgatgaccgt ctggtgggcg aacgaactca
    16921 agaacaagaa cgacctcaag atcggccaga cgctgacgat cccgccggtc aacggcctcg
    16981 tcgtcacggt ccggacgagc gacaccctcg agacgctcgc tgcgaagtac cacgccgacc
    17041 cggccgacat cgtcgccgtc aacgagctcg ccgatccggt cctggtcgtc ggccagaccc
    17101 tgacgatccc tggcgcccgg tcggctcaga tcccgacgcc gaagcccacc aaggcgccga
    17161 ccaagaagcc cgtcgtccac tcgtcgggtg gcggcaacag cggttcgacg cgaccgccgg
    17221 cgtcctactc gggcggcaag ttcgcctggc ccgtcgccgg cggctacatc agccagtact
    17281 tccactacgg gcactacgcg atcgacatcg ccgccgacca gggcacgcgc gtgacggcgg
    17341 cggcgtccgg gtcggtcatc ttcgccggct ggaagaacaa tggcggcggc taccaggtct
    17401 ggatcgccca cggctccggg ctgtacacga cctacaacca catgtcctcc atctcggtcg
    17461 ggcgcggcca gtccgtcggc cgcggccagc aggtcggacg ggtcgggatg accggcaatg
    17521 cgaccggccc gcatctccac ttcgaggtct ggcgggggat gatctgggac ggcggcgtcc
    17581 gggtcaaccc gttgatctac ctctgatcga ctgacccgtc ccgcgacgcg ccgtcggctc
    17641 ggaggagggc cgacgacgcg gcgcgggccg gtcgcccgga acgatcgcca tcggcggctt
    17701 ggagctcgcc cgatggcgat gatccgccgg tgatcgtggt cggaccgatc ggatgcgaca
    17761 atccagctga tgtttctcga ccgcgtcaag atcttcgtca aggccggcgc gggcggtgac
    17821 ggtgcggcga cgttccgcaa ggaggcccac gtcccccggg gcggccccga cggcggcgac
    17881 ggcggtcgcg gcggctcgat ccacgtccag gtcgatgccg gccagacgac gctccgcgac
    17941 ttccggttca agcaccattt caaggcgaca tccggcggcc gcggctcggg atcccgccga
    18001 catggcgcag ccggcgacga gctgtttctt gacgtcccgc cgggcaccgc gatcttcgac
    18061 gacgagaccg gcgagctcct cgccgacctg gtcgcggtcg gccaggtcgc gatgatcgcc
    18121 cgcggcggcc gcggcgggct cggcaacacc cacttcaaga cgtcgaccca ccaggcgccc
    18181 aagcacgccc agaagggcga gcccggcgag gaacgctggc tccgcctgga actccggctg
    18241 atcgccgaca tcgggctcgt cgggctgccg aacgcgggca agtcgacact gttggccgcc
    18301 ctgaccgcgg ccacgccgaa gatcgcggac tacccgttca cgacgctcga gccgaatctc
    18361 ggagtgatgg acctcgccgg cccggacgat ccggccggcg acgagcggcg gccctcgatc
    18421 gccgacgtgc cgggcctgat cgagggcgct tcgctcggtg ccggcctcgg ccatgcgttc
    18481 cttcgtcacg tcgagcggac gcggatcctc atccatatgg tcgacggctc cggccgcgat
    18541 ccggaatggg atcacgacgt catccgcgag gagctccggg cccacgaccc ggcgttgctc
    18601 gagaagccgc ttctggtcgt cttcaacaag ctcgaccagc cgacggcggt cgaggcctgg
    18661 ccggccttcc gcgccgcgcg cctcgccgcc gggcaggacg tcgtcgcgat ttcggcgacc
    18721 gatgggacgg gcgtcgacga gttgcgcgat cggatcgccg acctcctgcc gggcgccgac
    18781 gagcgcgacc taccgcccga accggccggg gtcgtcgtcc accggatcga ggcgatgggt
    18841 gacggcttca gcgtcgaaca gctggaggac ggcgtcttcc gggtccgcgg caagcggatc
    18901 gaacgggtcg ccgcccagac gaacttcgag gtcgaggaat ctgccgagcg cttccagcgc
    18961 gacctcgccc ggctcgggat cgacgacgag ttgcgacggg ccggggtcac ggccggcgac
    19021 gtcgtcagga tcggcgcgac cgagctgaca tgggaggcgc agccctggga ggagcggtga
    19081 cggccgcgcc cgggccggtt caggtccggc cgggatcgat cggcatcctc ggcggcacct
    19141 tcgacccgtt ccacgccggc cacctggccc tcgcccgggc ggcccgcgac cagctcgggc
    19201 tcgagcgcat cctcgtcgtc ccggctgccg tgccgccgca caagcgggat cggccgatca
    19261 gccctgctgc cgtccggctc gccctggtcg aggccgggat cgcgggcgag ccgcggctcg
    19321 aggcgagccg gatcgagctc gaccgcgacg ggccgtcgtg gaccgtcgtc acggtcgccg
    19381 acctggcagc cgcggagcgt gcggccggac gcgagcccga cctgaccgtg atcctgtcgg
    19441 tcgagtcgtt cgccggcctt ccgacgtggc acgagccgtt gcgcgtgctc gacctcgccc
    19501 ggatcgccgt cgctccgcga gccggccacg agccgcccga tccggcctgg atcgcggcga
    19561 acttccccgg tcgtgaggac cggatcctcg tcatcgacgg ccccagggtc gacgtctccg
    19621 cgtcggaggt ccggcgtcgt gccgccaccg gcgaatcgct cgccggcctc gtcccgccgg
    19681 gcgtcgcgaa ggccatcgcc gccgatcggc tgtatcgtca ccccgaaccc caggaggatg
    19741 aatcgatcgt gactgactcc gcgcccctcg ccgggacgcc cgccgaccag ccgtcggcaa
    19801 ctgcccatat cccccgcccg gacggcctgc ccagccgcga tgcggccgcc gccgtggtcc
    19861 ccgccgctga gcggccgtcc ctcgacattg cccggcggat cgtcgagctg gccgaggaca
    19921 agaaggcggc ggacatcatc ctgctcgacc tgaccgggct cacgacgctg gccgatgcct
    19981 tcgtgatctg ctccggaggc tccgagcgcc agctcgacgc gatcgcggac ggcgtcatcg
    20041 aaggcatgcg gtccgagaag gtccgcccga tcggccgcga ggggaccgcc gcgtcgcact
    20101 gggtcctggt cgacttcggg gcggtcgtcg tccacatctt cacgcccccg gagcgcgagt
    20161 actactcgct cgagaagcac tgggccgagg cgcgaaccgt gctgcgagtt cagtagctgt
    20221 cgcacctgag cacgccatcg cggcgttgcg ccctgcgctc ctcgttcacg accatccgag
    20281 gtcgctcgct ccggtgctcg ggcgcgcctt gcgctgacgc actcatgcgc gacgaacgca
    20341 gtcgcgtgtc tggctctgcg cgcggcggct cggctcctga tcacgaatct cggacggaat
    20401 ccctagcccg agtaccgccc ccgtggcggt accgtcgggt catgtcggcg ggcggtgctg
    20461 gacggcagca tccgtggcag gtggtcgatc gatgatcgac ccggccacga ggctcagcag
    20521 atccgccgat gtccc
//
LOCUS       DATAIW010000077         9860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_92726, whole genome shotgun sequence.
ACCESSION   DATAIW010000077 DATAIW010000000
VERSION     DATAIW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92726"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VHM48_02605"
     CDS             complement(<1..229)
                     /locus_tag="VHM48_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754322.1"
                     /translation="MRRLEGAGHLGGDALPHLGQRNENLVGAAVKAAPGIEFPAGADE
                     VLVPLTQMRKGIAAQMTRALQAPHAYVQMEID"
     gene            203..856
                     /locus_tag="VHM48_02610"
     CDS             203..856
                     /locus_tag="VHM48_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX2754323.1"
                     /translation="MTRALQAPHAYVQMEIDATRLVAFREKNKNGYQAKEGIGLSFVP
                     FVVKAVAVALKKTPTFNANWTEQGLMAKRRVNIGVAVAVDDGLIVPVIRDADQLSIHG
                     LNVAIADVAARAKANKLRLDDFGGGTFTVDNTGYLGTNLVMPILNVPEVGIVTMEAIT
                     KRPVVVSTPDGDVIAIRPVMNMVLGVDHRANDGAGGAALLRDIKAWLESVGPDTAIY"
     gene            876..1832
                     /locus_tag="VHM48_02615"
     CDS             876..1832
                     /locus_tag="VHM48_02615"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2754324.1"
                     /translation="MRLVVTGGTGLVGGAVIRVLRDRGDTVVAIVRDPNRAGHLAELG
                     AVLVQDDLSAVAGLTELLRGSDGVIHAAGSYRIGIPKSEHGAMWEANVGTTTRVFDAA
                     EAAATPRIIYVSTVNIYGNTHGATVDETDRRDLGEAFLSWYDETKYGAHEVAEQRIAA
                     GAPIVIVLPSQVYGPGDRSLFGEQLRLAHAGKLRYRAVDDVRIGLVHADDLAAGIAAA
                     LDRGMPGQEYNLPGPTASLGEAIAIAARLGGHTAPRLRIPIGVLNAMAPLGRLIGGPN
                     LAEIVSASDGVTYLMSPAKAEAELGFTTRDIETGFRDTFGPA"
     gene            1847..2893
                     /gene="lipA"
                     /locus_tag="VHM48_02620"
     CDS             1847..2893
                     /gene="lipA"
                     /locus_tag="VHM48_02620"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2754325.1"
                     /translation="MARELPIFRPPGADAGPHGAHVPDTIAVGGVGPAALTLLRRHPD
                     WIKARMPAGESYHDLKGLLRGLNLNTVCEEAHCPNIGECWDQRTATIMILGDTCTRAC
                     GFCAVKTGRPTWFDDDEPRRVAEAVGQLGLEHVVVTSVARDDLPDGGARIFAETILAM
                     RATSPGMGIEVLIPDFDGADERLRTVMDARPDILNHNLETVKRLQKPVRKRARWDRSL
                     YVLQRAKEMAGEIGYPVHTKSSLMVGLGETRPELTEAFGALREVDVDILTVGQYLRPS
                     EQHLPLERYYHPDEFADMKREALALGFKHVESAPLVRSSYHARDQVPGAELRALRRRQ
                     ATLDADGRIVPAAS"
     gene            3049..4539
                     /locus_tag="VHM48_02625"
     CDS             3049..4539
                     /locus_tag="VHM48_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754326.1"
                     /translation="MATLIGLAVLKPWDAPHPSTTGPRGSIDPTTPGLSIAGPSAAAV
                     AFDYPTPAELIAASVPREAWGIRAIVLPSGVRPAGDGTGTGLDDRWLSIDVGAGAAWD
                     AGVTGTAVDEGDAVLAIGITTPPDAMPLDVRFARLEPGDAVRRVVPQAIRGVEPGSWL
                     WSPDPTEATAAGGWPAGTYVIDVLLGPRIVRLVTIVPSGVPAVARKPPVEPDPQRIGD
                     QLSGFDAGPFALGPRGPIAIDVIAHDLLDERQAWLGPATGNLEIRAVGRVVADPVFSL
                     GLVLEPGEQLVTVGLDRVAPVGEPMDLVEVPGPLVPTSTDSPMTSVLLSFADRDVLGP
                     GLYHLTAVTRRPSGEERSRTWNIEIDPATIPSPPGFVLDQMARWVPLMQDPDRVAGQP
                     LITDRDLGGTDGDGTCGGSAKISPFDELIGIVDPAGERITAIRVTPIDTIRRADVAVR
                     IAPEAVDGLSLVALPSGGIAAHNYAVIAATEGPLGSGRQIYTICVG"
     gene            4604..6202
                     /locus_tag="VHM48_02630"
     CDS             4604..6202
                     /locus_tag="VHM48_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754327.1"
                     /translation="MNDHVDGPDGRPTELVIEPTRLGGRRSTRRGLVTAVVATIFIGA
                     AVWKPWDTRPAAPGASPGVAPASAPTASGDVAFGSPPPSGSGHVLPVDGEPFPTNAEL
                     LAATTRQPTWGVRAMVFRTGGPIFTGQPNLVERWVGVPANVGDVSPALSALAIAQPDD
                     DVAAIGVTTLDDALALDVRFWSLPPNGPAQRLAAIAIQGPEAGSWLWLADPAHATDRG
                     TWQAGAYGIDVLLGPRIVRLRATIPSASGTTTRVATPFGQPPFATILDSLEPGPFALA
                     DGGAEPLVGGSPPVVDEREAWLGPAAGLPPVAHVAARQVTGFGMLFAAGDEPVRVHVR
                     QLAPLSSPIETGVNIVTVEPGHRRAVIAWPVSGGVVPDGLYHMTVSWTSAGADHSAES
                     VIEVQPQVVARPPNAPLDAMTRWVGLIDRPDIAAREPLVFVDDTTTPDRCSVAPVISG
                     SDPLFGVVAPPRVSVVGVRLRPSGVAEAADVPIRFAPDAIPRLTVVALPSAGLPVRAY
                     DLIVTLGSAAGERDVTRRVCVSAP"
     gene            6296..7351
                     /locus_tag="VHM48_02635"
     CDS             6296..7351
                     /locus_tag="VHM48_02635"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEX2754328.1"
                     /translation="MARQLSRKIPLAELHCHLGGAVTPAIMWSIAHAQGIKLPTRDYW
                     EFRDTITVSEKRNRSFDGYLQLFHWTELIQSSPIAVERAVYEVVGGAYRKNNVTTTEL
                     RFNPMKRNRGGEQDLDHIIAAAARGMDRASLEYPVKPGLIFCLDRAFPFELNEIIVEK
                     AITWRDRGVVGVDIAGPESATFRVADYGRLFRRARQFGLGLTVHTGESGPVDEVARVV
                     ELLEPDRIGHGVKAAYDPRAMAMIRERGIVLEICPSSNLNTRVVSGWEEFRWIFDTFR
                     RNEVRFTINTDGPEMLKTYIRDEMASLGRYGILTVEDQEQAAATSLAASFVPNVSDVP
                     AARMEPATRVAVEREEA"
     gene            7471..7995
                     /locus_tag="VHM48_02640"
     CDS             7471..7995
                     /locus_tag="VHM48_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754329.1"
                     /translation="MSTLSVARRGRLLLASVAVVVALAVPTAALADTTPAPTIYPAAS
                     NHATIRLTGGSVIGRVVVNTQVSFTCDPFLVFDWETGSEVEVTTGSLEYGAVTIVQAS
                     GRTINSGEGTFFGGDVVCDGTTVNLRDVAVVAGLAPWKAGMAVAGAHISISSPDFNSS
                     SYASTGAMSLKLGR"
     gene            complement(8027..8737)
                     /locus_tag="VHM48_02645"
     CDS             complement(8027..8737)
                     /locus_tag="VHM48_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754330.1"
                     /translation="MDPATEHAASPVASRAEPTTSSGSPRVLRREILDRLRREGPASP
                     DQLAAAIGASRTGVLQQLRALEAANFVSRQTVRHGVGRPRHLYDVTADAQELFPSNYD
                     GLAAGLLAAIGALGGDQLLEQVFQARRRQIGDRIRREIDDRVGAAASLAERVGALAVL
                     QDEQGYLADAVIDADGTIRLREHNCAIFDVARGQRSACDAELDLFREVLGADVVRDTH
                     IASGDRCCSYRIEERAGD"
     gene            8794..>9860
                     /locus_tag="VHM48_02650"
     CDS             8794..>9860
                     /locus_tag="VHM48_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2754331.1"
                     /translation="MDFDLSPEHSLLRSTVRGFMETEVAAVIDEHEKARRFPSEIVKR
                     LGEMGWLGIPVAEEDGGAGMDTLAYAIAVEEISRVWGSLGIIVAAHTSLGCGPLMIAG
                     TAEQKERYLRPLASGAVIGAYGLTEPGAGSDAGGTKTTARRETTPDGDSWVIDGGKRF
                     ITNAGQAGTYIVTAKTGETAKGDAEISAFIVPADTAGFSIGRLEDKMGLHASATGELI
                     FDGCRIPLENLLGAQGEGFRMFLKVLDGGRISIAAMALGLAQGAYEAAAAYSKERKQF
                     GRAIGTFQGVAFMIADMATEIEAARALVYRAAWLKDQHRDYTVAGAQAKLFASEVSSR
                     VTNAAVQVHGGYGYITEYKVER"
BASE COUNT         1412 a         3573 c         3431 g         1444 t
ORIGIN      
        1 cgtcgatctc catctggacg taggcgtgag gcgcctggag ggcgcgggtc atctgggcgg
       61 cgatgccctt ccgcatctgg gtcagcggaa cgagaacttc gtcggcgccg gccgggaatt
      121 cgatgccggg cgcggccttg accgcggcgc cgacgaggtt ctcgttgcgc tgacccagat
      181 gcggaagggc atcgccgccc agatgacccg cgccctccag gcgcctcacg cctacgtcca
      241 gatggagatc gacgcgacga ggctggtcgc cttccgcgag aagaacaaga atggctacca
      301 ggcgaaggag gggatcggcc tcagcttcgt cccgttcgtc gtcaaggccg tggccgtcgc
      361 cctgaagaag acgccgacat tcaatgccaa ctggaccgaa cagggcctga tggccaagcg
      421 ccgggtgaat atcggcgttg ccgtggcggt cgatgacggc ctgatcgttc ccgtcatccg
      481 cgacgcggac caactctcga tccatggcct gaatgtcgcg atcgccgacg ttgccgcccg
      541 ggcgaaggcc aacaagctcc gcctcgacga ctttggcggc gggacattca ccgtcgacaa
      601 caccggctat ctcgggacga acctcgtgat gccgatcctg aacgtccccg aggtcgggat
      661 cgtcacgatg gaagcgatca cgaagcgccc cgtcgtggtc tcgacgccgg acggcgacgt
      721 gatcgccatt cgacccgtga tgaacatggt cctcggcgtc gaccaccggg ccaacgacgg
      781 ggccggcggc gcggccctcc tgcgcgacat caaggcctgg ctcgagagcg tcggaccgga
      841 cacggcgatc tactgagcgc catccggacc ggctcatgag gctcgtcgtc accgggggaa
      901 ccggtctggt cggcggggcc gtgatccggg tcctccgcga tcgcggcgac accgtcgtcg
      961 ccatcgtccg cgacccgaac cgcgccggcc atctcgccga gctcggggcg gtgctggtcc
     1021 aggacgatct ttcggcggtc gccggtctca ccgagctcct gcgcggttcg gacggcgtca
     1081 tccacgccgc cggcagctac cggatcggga tcccgaagtc cgagcacggg gcgatgtggg
     1141 aggcgaacgt cgggacgacc acccgcgtgt tcgatgctgc cgaggcggct gcgacgccgc
     1201 ggatcatcta cgtctcgacc gtcaacatct acggcaatac ccacggcgca acggtcgacg
     1261 aaaccgaccg gcgggacctc ggcgaggcgt tcctgagctg gtacgacgag acgaagtacg
     1321 gcgcccacga ggtcgccgag cagcggatcg ccgccggcgc cccgatcgtg atcgtcctgc
     1381 cgagccaggt ctacgggccc ggcgaccggt ccctgttcgg cgagcagctc cggctcgccc
     1441 acgcaggcaa gctccgctac cgggcggtcg acgacgtccg gatcggtctc gtccacgccg
     1501 acgacctcgc ggccggcatc gccgccgcgc tcgatcgggg catgccgggg caggaataca
     1561 acctgccagg gccgacggca agcctcggcg aggcgatcgc gatcgccgct cggctcggtg
     1621 gtcacaccgc gccccggctg cggatcccga tcggcgtcct caatgccatg gcgccgctcg
     1681 gccgcctgat cggagggccg aacctcgccg agatcgtgtc ggcgtcggac ggggtcacct
     1741 atctcatgtc tccggccaag gccgaggccg agctcggctt cacgacccgc gacatcgaga
     1801 cggggttccg cgacacgttc ggcccggcct gactagactc acgtcgatgg ctcgcgaact
     1861 cccgatcttc cgtccgcccg gggccgatgc gggcccccac ggcgcgcacg tccccgacac
     1921 gatcgcggtc ggcggtgtcg gtccggcggc gctgacactc ctccgtcggc accccgactg
     1981 gatcaaggca cggatgccgg cgggggagtc ctaccacgat ctgaagggtt tgctccgcgg
     2041 cctcaacctc aacacggtct gcgaggaggc ccactgcccg aacatcgggg agtgctggga
     2101 ccagcggacc gcgacgatca tgatcctcgg cgacacgtgc acccgggcct gcggcttctg
     2161 cgccgtcaag accggtcgtc cgacgtggtt cgacgacgac gagccgcgtc gcgtcgcgga
     2221 ggcggttggc cagctcggcc tggagcacgt cgtcgtcacg agcgtcgctc gcgacgacct
     2281 gcccgacggt ggcgcgcgga tcttcgccga gacgatcctc gcgatgcggg caaccagccc
     2341 aggcatgggc atcgaggtcc tgatcccgga cttcgacggc gccgacgagc ggctccggac
     2401 cgtgatggac gcgcggccgg acatcctcaa ccacaacctc gagacggtga agcgcctcca
     2461 gaagccggtc cgcaagcgcg cccgctggga tcgcagcctg tacgtcctcc agcgggcgaa
     2521 ggagatggcg ggcgagatcg gctaccccgt ccacacgaag agctcgctca tggtcgggct
     2581 cggtgagacg cgcccggagc tgaccgaggc cttcggggcg ctccgcgagg tcgacgtcga
     2641 catcctgacg gtcggccagt acctccgccc gtccgagcag cacctgcccc tcgagcgcta
     2701 ctaccatccc gacgaattcg ccgacatgaa gcgcgaggcg ctcgcgctcg ggttcaaaca
     2761 cgtcgagtcg gcgcccctcg tccgctcgag ctaccacgcc cgcgaccagg tccccggcgc
     2821 cgagctccgg gctcttcgtc gtcgccaggc gacgctcgat gcggacggcc ggatcgtccc
     2881 cgcggccagc tgacgtcgca gccgccgagg aagcccgaac ggatcggcgt ggaaccgggc
     2941 gtcgtcggcg ccgtgacgcc tgacacccgg cccgagccgg ggctgcgggt cgcgccagct
     3001 cgggtcagca ggcgacgatc gctggtcccg ctgatgcttg ctgcggcaat ggcgacgctg
     3061 atcgggctgg ctgtcctcaa gccctgggac gcgccgcatc catccacgac cggccctcgc
     3121 ggatcgatcg acccgacgac gccgggtctc tcgatcgcgg gcccgtcggc ggcggcagtc
     3181 gcgttcgact accccacccc cgccgaactc atcgccgcgt cggtgccgcg agaggcgtgg
     3241 gggatccggg cgatcgtcct gccgtcaggt gtccggccgg cgggcgacgg cacgggcacc
     3301 ggcctggacg accggtggtt gtcgatcgac gtcggggccg gtgcggcctg ggacgccggg
     3361 gtgaccggca cagccgtcga cgagggcgat gccgtcctcg ccatcggcat cacgactccg
     3421 cctgacgcga tgccgctcga cgttcgtttc gcccggctgg agccgggcga tgccgttcgc
     3481 agggtcgtgc cccaggccat ccgcggcgtc gagcctggat catggctctg gtcgccggac
     3541 ccgaccgagg cgaccgccgc cggcggctgg ccggccggga cgtacgtcat cgacgtcctg
     3601 ctcggtccgc ggatcgtccg tctcgtgacc atcgtcccgt ccggcgtgcc ggccgtcgcc
     3661 cggaagcccc cggtcgagcc cgatccgcaa cggatcggcg atcagctcag cgggttcgac
     3721 gcgggcccat tcgcgctcgg cccacgaggt ccgatcgcga tcgacgtcat cgcccacgat
     3781 ctgctcgatg agcggcaggc ctggctcggt cccgcgaccg gcaacctgga gatcagggcc
     3841 gtcggcaggg tcgtcgcgga cccggtgttc agcctcggct tggtgctcga gcccggcgaa
     3901 cagctcgtca cggtcggcct cgatcgcgtg gcgccggtcg gtgaacccat ggacctggtc
     3961 gaggttccgg ggccgctcgt ccccacctcg accgattcgc cgatgaccag cgtcctcctt
     4021 tcctttgccg atcgggacgt gctcggcccg ggcctctatc acctgaccgc cgtcacccgc
     4081 cggccgtccg gcgaggagcg atcgcggacg tggaacatcg agatcgaccc ggcgacgatc
     4141 ccgtcaccgc caggtttcgt gctcgatcag atggcccgct gggtgccgct gatgcaggat
     4201 cccgatcgcg tcgccggcca gccgctcatc accgaccgcg atctcggggg gaccgatggc
     4261 gacggaacct gtggcgggtc ggcgaagatc agcccattcg acgagttgat cgggatcgtc
     4321 gatccggcag gcgagcggat caccgccatt cgggtgactc cgatcgatac gatccggcgc
     4381 gcggacgtgg cggtccggat cgcaccggag gcggtcgacg gcctgagcct ggtcgcgctg
     4441 ccgagcgggg gcattgccgc ccacaactac gctgtcatcg ccgcgacgga gggcccactg
     4501 gggtccggtc gtcagatcta cacgatctgc gtcgggtgat gcggcccgtg gcggcgcgcc
     4561 gacaccgtcg tcccaccgcc gaccagccgg gcgtctcccg tccgtgaacg accacgtcga
     4621 cggacccgac ggcaggccga ccgagctcgt catcgagccc acccggctcg gcgggcggcg
     4681 ctcgacccgg cgcgggctcg tcacggcagt ggtggcgacg atcttcatcg gcgcggccgt
     4741 ctggaagccg tgggacaccc ggcccgcggc gccgggtgcc agtccgggcg tggcgccggc
     4801 ctcggcgccg acggcatcgg gtgacgtggc atttggatcc ccgccaccga gcggctccgg
     4861 gcacgtcctc ccggtcgacg gagagccatt ccccacgaat gccgagctcc ttgcggcgac
     4921 gacgcgccag ccgacctggg gggtccgggc aatggtcttc cggaccggcg gcccgatctt
     4981 caccggccag cccaacctgg tcgagcgctg ggtgggggtc ccggccaacg tcggcgatgt
     5041 atcgcccgcc ctgtcggcgc tggccatcgc ccagcccgac gacgacgtcg ccgcgatcgg
     5101 cgtgaccacc ctcgacgacg cgctggcgct cgacgtccgg ttctggagcc tgccgccgaa
     5161 cgggcccgcg cagcggctcg ctgcgatcgc gatccaaggg cccgaggccg ggtcgtggct
     5221 atggctcgcg gatccggcgc acgccaccga tcgcgggacc tggcaggccg gcgcctacgg
     5281 gatcgatgtg ctcctcggcc cgcgcatcgt ccggctccgg gcgacgatcc cgagcgccag
     5341 cgggacgacg actcgcgtcg cgaccccgtt tggccagccg ccgttcgcga cgatcctcga
     5401 cagcctcgag cccgggccgt tcgcgctggc cgacggcggc gccgagcccc tggtcggcgg
     5461 cagcccgccg gtcgtcgacg agcgcgaggc gtggctcggt cccgccgcgg ggctcccgcc
     5521 tgtcgcccac gtggccgccc gccaggtgac cggcttcggc atgctcttcg ccgccggtga
     5581 cgaaccggtc cgcgtccacg tgcgccagct cgcgccgctg tcctcaccga tcgagaccgg
     5641 cgtcaacatc gtcaccgtcg agccgggcca ccgccgggcc gtcatcgcct ggcccgtctc
     5701 cggcggcgtc gtccccgatg gcctgtacca catgacggtc agctggacgt cggccggcgc
     5761 ggatcattcg gcggagtcgg tgatcgaggt gcagccgcag gtcgtggctc gcccgccgaa
     5821 tgcgcccctc gacgcgatga cgcgttgggt cgggttgatc gaccgccctg acatcgcggc
     5881 gcgcgagccc ttggtcttcg tggacgacac gacaacgccc gacagatgct cggttgcgcc
     5941 ggtgatcagc ggcagcgatc cgctgttcgg ggtggtcgcg ccgccgcgcg tgtcggtcgt
     6001 cggggtccgc ttgcgtccga gtggcgtggc cgaggccgcg gatgtcccga tccgcttcgc
     6061 gcccgacgcg atcccgcggc tgacggtggt ggcgttgcca tcggccggcc tgccggttcg
     6121 cgcctacgac ctgatcgtga ccctcggctc ggccgccggc gagcgcgacg tcacccggcg
     6181 ggtctgcgtc agcgcccctt gatgatcgtc gggctcctgc cgttcgtcct cggcaacctc
     6241 agcagggccc accggcggcc acgctgaccg ctcggtcgtc cggtatcctc cggtcgtggc
     6301 ccgtcagctc tcccgcaaga tcccgctcgc cgagctccac tgtcacctcg gcggggcggt
     6361 gacgccggcg atcatgtgga gcatcgccca cgcccagggg atcaagctcc cgacccgcga
     6421 ctactgggag ttccgcgaca cgatcaccgt gtcggagaag cgcaaccgca gcttcgacgg
     6481 ctacctccag ctcttccact ggacggagct catccagagc tccccgatcg cggtcgagcg
     6541 cgcggtctac gaggtcgtcg gcggtgccta ccgcaagaac aacgtcacga ccaccgagct
     6601 ccgcttcaac ccgatgaagc gcaaccgcgg tggcgagcag gacctcgacc acatcatcgc
     6661 ggccgccgcc cgcgggatgg accgggcgag cctcgagtac ccggtgaagc ccgggctgat
     6721 cttctgcctc gaccgggcgt tcccgttcga gctcaacgag atcatcgtcg agaaggcgat
     6781 cacgtggcgc gatcgaggtg tggtcggggt cgacatcgcc ggacccgagt cggccacgtt
     6841 ccgcgtcgcc gactacggcc gcctcttccg gcgggcgcgc cagttcggtc tcgggctgac
     6901 cgtccatacc ggcgagtccg gcccggtcga cgaggtcgcc agggtcgtcg agctgctcga
     6961 gcccgaccgg atcggccatg gcgtcaaggc tgcctacgat ccgcgggcga tggcgatgat
     7021 ccgcgagcgc gggatcgtcc tcgagatctg cccgagctcg aacctcaaca cccgggtcgt
     7081 gtccggctgg gaggagttcc gctggatctt cgacacgttc cggcggaacg aggtccggtt
     7141 cacgatcaac accgacggcc cggagatgct caagacctac atccgcgacg agatggcgtc
     7201 gctcggtcgc tacgggatcc tgaccgtcga ggaccaggag caggccgccg cgacatccct
     7261 cgcggcgtcc ttcgtgccga acgtctcgga tgtgccagca gcccggatgg agcccgcgac
     7321 ccgcgtggcc gtcgagcgcg aagaggcctg acgctccgca gccgtcgccg ccgtccgccg
     7381 ggcccgctgt cacgcgcggc cgtcccgttc gtccaccgca cggatcgatg gcgcaccttg
     7441 aggcgccggt ccaccggagg tcaggacgtc atgtccaccc tgtctgtagc ccgccggggg
     7501 cggcttctcc tcgcgagcgt tgcggtcgtt gtcgcgctcg ccgtgccgac ggccgcgctc
     7561 gccgatacca ccccggcgcc gacgatctat cccgcggcgt ccaaccacgc gacgatccgg
     7621 ctcacgggcg gctcggtcat cggccgggtg gtggtgaaca cgcaggtcag cttcacctgc
     7681 gatccgttcc tcgtcttcga ctgggaaacc ggcagcgagg tcgaggtcac caccggctcg
     7741 ctcgagtacg gcgccgtcac gatcgtccag gcctccggcc ggacgatcaa ctcgggcgag
     7801 ggcacgttct tcggtggcga cgtcgtctgc gatggaacga cggtcaacct gcgcgatgtc
     7861 gccgtcgtcg ccggtctcgc cccgtggaag gcgggaatgg ccgtcgccgg agcgcacatc
     7921 tcgatctcga gccccgactt caattccagt tcctacgcca gcacgggcgc catgtccctg
     7981 aagctcggcc gctgaacggt cgccgatcgg cgaacgcgcc gcgggctcag tccccggcgc
     8041 gttcctcgat tcggtacgag cagcagcggt cgccgctcgc gatgtgggtg tcgcggacga
     8101 cgtcggctcc gagcacttcg cggaacaggt cgagctccgc atcgcaggcc gagcgctgac
     8161 cacgagcgac gtcgaagatc gcgcagttgt gctcgcgcag gcggatcgtc ccgtccgcgt
     8221 cgatcaccgc atcggcgagg tagccctgct cgtcctggag cacggccagc gcgccgacgc
     8281 gctcggccag gctcgcggcg gcgccgaccc ggtcgtcgat ctctcgccgg atgcgatcgc
     8341 cgatctgtcg tcgtcgcgcc tggaagacct gctcgaggag ctgatcgcca cccaacgccc
     8401 cgatcgcggc caggagcccg gccgcgagac cgtcgtagtt ggacgggaag agctcctggg
     8461 cgtccgccgt gacgtcatac aggtggcgcg gccggccgac cccgtggcgg acggtctggc
     8521 ggctgacgaa gttcgcggcc tcgagcgccc gcagctgctg gaggacgccc gtccggctgg
     8581 cgccgattgc cgcggccagc tgatccggag acgccggtcc ctcgcggcgg aggcggtcga
     8641 ggatctcgcg gcgcaggacg cgcggcgagc ctgacgacgt ggtgggctcg gcccgtgacg
     8701 cgaccgggga agcggcgtgc tcggtggccg ggtccatggc ccgaaggcta gcaccgggac
     8761 cgatcgagcg cgtccggtgc taccctcggc cgcatggatt tcgacctgtc ccccgagcac
     8821 agcctcctcc gctcgacggt ccgcggcttc atggagaccg aggtcgccgc tgtcatcgac
     8881 gagcacgaga aggcgcgtcg cttcccgtcc gagatcgtga agcgactcgg cgagatgggc
     8941 tggctgggca tccccgtcgc cgaggaggac ggcggggcgg gcatggacac gctggcctac
     9001 gcgatcgcgg tcgaggagat cagccgggtc tggggctcgc tcgggatcat cgtggctgcc
     9061 cacacgtcgc tcggctgcgg gccgctgatg atcgccggca cggccgagca gaaggagcgt
     9121 tacctccggc cgctcgcctc gggtgcggtg atcggcgcct acgggctgac cgagccgggc
     9181 gccggctcgg atgccggcgg gacgaagacg acggcccgcc gcgagacgac ccccgacggc
     9241 gacagctggg tcatcgacgg cggcaagcgc ttcatcacca acgccggcca ggccgggacc
     9301 tacatcgtca ctgcgaagac gggcgaaacg gccaagggcg acgcggagat cagcgcattc
     9361 atcgtccctg ccgacacggc aggcttctcg atcggccggc tcgaggacaa gatgggtctc
     9421 cacgcgtcgg ccacgggcga actcatcttc gatggttgcc ggatccccct cgagaacctc
     9481 ctcggcgccc agggcgaggg cttccggatg ttcctcaagg tcctcgacgg cggtcggatc
     9541 tcgatcgccg ccatggccct cgggctggcc cagggtgcgt acgaggcggc ggctgcctac
     9601 agcaaggagc gcaaacagtt cggccgggcg atcgggacct tccagggcgt cgcgttcatg
     9661 atcgccgaca tggcgaccga gatcgaagcc gcccgcgccc tggtctaccg tgccgcgtgg
     9721 ctcaaggacc agcatcgcga ctacaccgtg gccggtgccc aggccaagct gttcgcctcg
     9781 gaggtcagta gccgggtcac gaacgccgcg gtccaggtcc acggcggcta cggctacatc
     9841 accgagtaca aggtcgagcg
//
LOCUS       DATAIW010000078         5707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_3146, whole genome shotgun sequence.
ACCESSION   DATAIW010000078 DATAIW010000000
VERSION     DATAIW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VHM48_02655"
     CDS             complement(<1..1281)
                     /locus_tag="VHM48_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2754332.1"
                     /translation="MILSVRNRVRSFGFDLTDRRFEILAYGAILVVAAFLRLGNLAAR
                     GTWDADQGHDMLVLRALVLDGRLPLLGPPTSIGDFHHGVLYYLLLAPAAAVSRADPIA
                     VTTWIALGGVVAVAVTGWLARSIAGPIAGLIAALVLAVSASAVEESIFIWNPNLIALS
                     SSIALAAAWRAWTGGRARWWLVAGAAAVVTMQCHVLGVILSPVIGGLLVADMRRRRRV
                     GDRRGASAVGRAAFGWLGIALLSYVPLAIHEIGNDASELRAAIAFVTGGGGPASVALA
                     ARLPIVGLRVVGWPLGGLITAAPMATVLAAALVAGMAIWRGWLAGRRFAETPDERTAV
                     RWLALGLAWTIVALAVGAASLATVVPGLPNDHYHAFADPMVVILVAVGLARLLARPTR
                     GASPVAAVGAAALIVALVGWNLASQPPSKASDGGW"
     gene            complement(1278..2432)
                     /locus_tag="VHM48_02660"
     CDS             complement(1278..2432)
                     /locus_tag="VHM48_02660"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2754333.1"
                     /translation="MLPDGRRLGAHLALGEGMVKAAERAVEIGLGALQVFSDNPTAWR
                     RRAGPSPEIPAFRSRLVEGGIAPLTIHASYLVNLAGSDPTYHERSVTLLAAELRSARR
                     FGATLVNVHIGSHGGAGIEAGIERLVSAVRRALDAENEPGSSGLPGSDEDEPDSDPPA
                     VESGASTTPATIVLENSAGGGFGLGVDLDELTAIADALDAAGVPRERIGFCLDTAHAW
                     GAGHDLSDPAVIDAFVDDLDRRIGLDRLPLVHLNDSRAELGSRLDRHEHIGAGKIGET
                     GMAHLLRHPRLAHATYILETPGMDEGYDAINVARAVALARGEPLAALPPEAFTLRSSR
                     ARAATPIDPSPVERSFDADPAHPSDRADRTEPSDSADPSGSVRPPGLAAS"
     gene            2474..2863
                     /locus_tag="VHM48_02665"
     CDS             2474..2863
                     /locus_tag="VHM48_02665"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEX2754334.1"
                     /translation="MNDRQAPIPGNPAAAGEASGAGGPDRRRAGEPDRRRLSDQEKVV
                     RAFFRDGRLVSIPAKTSKRDLLLPVILDRCFPDDRDYEEKEVNMRLALLHPDVAALRR
                     HLVDGRLMTREAGIYRRARSLTTDPAG"
     gene            complement(2913..3656)
                     /gene="raiA"
                     /locus_tag="VHM48_02670"
     CDS             complement(2913..3656)
                     /gene="raiA"
                     /locus_tag="VHM48_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX2754335.1"
                     /translation="MDSGGFGTVRIRPGGRLGRRRLDRRAAPGNIPNRHQREATGLWE
                     ITVRTIVKGKNIDVPDHVRRYAERKLARLERLLDDRSDALVELSIEQHRSAAVSHIVE
                     VTLTIDGKTLQTHAAAETHRAGVDEVVDKMERRAVDHLEKPRIRSRPDEEKVLLRQIA
                     DGTSDGGSERRIVKSKRFAIEPMFEEDALTRMEELGHQFFVYVDAQSERVQILYRRRD
                     GNFGMIEPVVAGEYTKGKGRGSDRSNGHR"
     gene            complement(3672..4406)
                     /locus_tag="VHM48_02675"
     CDS             complement(3672..4406)
                     /locus_tag="VHM48_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754336.1"
                     /translation="MSPAASLPNRLLDLAFPARCPGCGREGAAICATCLPALDARLDQ
                     PAGTPIGLPSDVPPSILQLDWCAPFGGLVRRALHQLKYGGETRLAVPLGGAIARRWAR
                     VTAGGDVLVPVPVHVDRARHRGYDQAELIARAAAAELGLPCAPILERARATIAQFELD
                     RATRATNVRGAFRLRPQPSGTRPAGTPAADTGQGRERPPRPLEGRWIVLVDDVVTTGA
                     TLSACAAPLLAAGAVGVSAVTVARER"
     gene            4541..5281
                     /locus_tag="VHM48_02680"
     CDS             4541..5281
                     /locus_tag="VHM48_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754337.1"
                     /translation="MTDDRAGRLAAAVRRHLGLRQIDVARMAKVDQKVVSLIERGQLE
                     RVSVQRFRAVCAVLGIEPVLDLRWRGGQVDRLIDRGHAHIVERVIAELGLLGWEVVPE
                     FTFNHFGERGSVDVLAWHPATRVLLIVEVKTRLTDLQAMLMSLSRKIRLVPGLVARDR
                     GWERRALGRIVVIADTHANRSTVATHRATFDASFPSRTAATRGWLRSPSGDLAGLWFI
                     AMEGHGVRGMEAGRRVRARRIAGPGGRD"
BASE COUNT          801 a         2004 c         2145 g          757 t
ORIGIN      
        1 ccagccgcca tcgctggcct tcgacggtgg ctggctcgcg aggttccagc cgacgagggc
       61 gacgatcagc gcggccgcgc ccaccgcagc gaccgggctg gctccgcggg tgggcctcgc
      121 gaggagtctc gccagcccga cggcgacgag aatgaccacc atcgggtcgg cgaaggcgtg
      181 gtagtggtcg ttggggaggc ccgggaccac ggtcgcgagg ctcgcggcgc cgaccgccag
      241 ggcgacgatc gtccaggcga ggccgagcgc cagccatcga acggcggtcc gctcgtcggg
      301 tgtctccgcg aaccggcggc ccgccagcca gccccgccag atcgccatgc ccgcgacgag
      361 ggcggccgcg aggacggttg ccattggagc cgcggtgatc agaccgccga gcggccagcc
      421 gacgacgcgc agtccgacga tcggcaggcg cgccgcaagc gccacgctcg ccggaccccc
      481 gccgccggtc acgaacgcga tcgcggcccg gagctccgag gcgtcgttcc cgatctcgtg
      541 gatcgccaac gggacatagc tcagcagcgc gatcccgagc cagccgaatg cggcccgacc
      601 gacggcagag gccccacggc gatccccgac gcgtcggcgc cgccgcatgt cggcgacgag
      661 gaggccgccg atgaccgggc tcaggatcac cccgaggacg tggcactgca tcgtgacgac
      721 cgcggccgcg ccagccacga gccaccatcg cgcgcgcccg ccggtccacg cccgccacgc
      781 cgcagcgagc gcgatcgagc tcgacagggc gatcaggttc gggttccaga tgaagatcga
      841 ctcctcgacc gcgctcgcgg agaccgccag gacgagggcc gcgatcaggc cggcgatcgg
      901 ccctgcgatc gagcgggcca gccagcccgt cacggccact gcgacgaccc cgccgagcgc
      961 gatccacgtc gtcaccgcga tgggatcggc ccgcgacacc gcagcggccg gcgcgaggag
     1021 caggtagtag aggacaccgt ggtggaagtc gccgatcgag gtcggcgggc cgaggagcgg
     1081 gagccggccg tccagcacca gcgcgcgcag gacgagcatg tcgtggccct ggtcggcgtc
     1141 ccacgtgccg cgggccgcga ggttcccgag ccgcaggaac gcggcgacga ccaggatcgc
     1201 gccgtaggcg aggatctcga agcgacggtc ggtgaggtcg aacccgaacg accggacgcg
     1261 gttgcggacg gagaggatca cgaagcggcg aggcccggtg gccggaccga gccggacgga
     1321 tccgcagaat cggacggctc ggtccgatcg gcccgatcgg atggatgcgc cggatccgcg
     1381 tcgaatgacc gctcgacggg cgatggatcg atcggcgtgg cggcgcgcgc ccggctgctg
     1441 cgcagggtga aggcctccgg cggcagcgcg gccaacggct ctccgcgggc gagtgccacc
     1501 gcccgggcga cgttgatcgc gtcgtacccc tcgtccatgc cgggggtctc gaggatgtag
     1561 gtcgcgtggg cgagacgcgg gtgacggagc agatgggcca tcccggtctc gccgatcttg
     1621 ccggcgccga tgtgctcgtg tcgatccagc cgcgacccga gctccgcccg cgagtcgttg
     1681 aggtggacca gcggcaggcg gtcgagcccg atccggcggt cgaggtcatc gacgaacgcg
     1741 tcgatgaccg cggggtcgga caggtcgtgg ccggcgcccc aggcatgggc ggtgtcgagg
     1801 cagaacccga tccgctctcg gggcacgccg gcggcatcga gcgcgtcggc gatggcggtc
     1861 agctcgtcga ggtcgacccc gagcccgaag ccgccgccgg cggagttctc gaggacgatc
     1921 gtcgcgggcg tggtcgaggc gcccgactcg accgccggcg ggtcactgtc cggttcgtcc
     1981 tcgtcgctgc cgggcaggcc gctcgaaccg ggctcgttct cggcgtcgag cgctcgccgg
     2041 acggcgctca cgaggcgctc gatcccggcc tcgatcccgg ccccgccgtg cgagccgatg
     2101 tggacgttga cgagcgtcgc accgaaccgc cgcgcggacc gcagctccgc cgcgaggagc
     2161 gtgaccgacc gctcgtggta ggtcgggtcg ctgccggcca ggttgaccag gtacgaggcg
     2221 tggatcgtga gtggggcgat cccgccctcg acgaggcgac tgcggaacgc cgggatctcg
     2281 ggtgacggcc cggcccgccg tcgccatgcc gtcgggttgt cgctgaagac ctggagggcg
     2341 cccaggccga tctccacggc ccgctcggcg gccttgacca tgccctcgcc cagggcaagg
     2401 tgggctccga ggcgacgtcc gtcgggcagc atcggtgcca tcgtagcggc cgccattctg
     2461 ctacggtcgc cgcgtgaacg accggcaggc cccgatcccg ggcaatcccg ccgccgcggg
     2521 ggaggcgtcc ggcgccggtg ggcccgatcg acggcgcgcc ggcgagccgg atcggcggcg
     2581 cctcagcgac caggagaagg tcgttcgcgc gttcttccgc gacgggcgcc tcgtgtcgat
     2641 tccggcgaag acgagcaagc gcgacctgct cctgcccgtg atcctcgacc gctgcttccc
     2701 cgacgatcgc gactacgagg agaaggaggt caacatgcgc ctcgccctcc tccatccaga
     2761 cgtggcggcg ctgcgccggc atctcgtcga cggccgcctg atgacacgcg aggccggaat
     2821 ctaccgccgc gcccgatcgc tgaccaccga tccggccggc tgacggaggg cggtccgcag
     2881 tcgggcctca acggtccgca gtcgggccgc ggtcagcggt gcccgttcga tcggtcgctg
     2941 ccgcgtccct tgcccttcgt gtactcgccc gcgacgaccg gctcgatcat cccgaagttg
     3001 ccgtctcggc gtcgatagag gatctggacc cgttccgact gggcgtcgac gtagacgaag
     3061 aactggtgac cgagctcctc catccgagtg agcgcgtcct cctcgaacat cggctcgatc
     3121 gcgaagcgct tcgacttgac gatccggcgc tccgaaccac cgtcggaggt gccgtccgcg
     3181 atctggcgga ggaggacctt ctcctcgtcg gggcgggacc ggatccgcgg cttctcgagg
     3241 tggtcgaccg cgcgtcgctc catcttgtcg acgacctcgt cgacgccggc ccgatgggtt
     3301 tcagcggcgg cgtgggtctg cagggtcttg ccgtcgatgg tgagggtgac ctcgacgatg
     3361 tgcgagaccg cggcgctgcg gtgctgctcg atcgagagct cgacgagggc gtcgctccgg
     3421 tcgtcgagga ggcgttcgag acgggcgagc ttgcgctccg cgtacctgcg gacgtggtcg
     3481 gggacgtcga tgttcttgcc cttgacgatc gtcctcacgg tgatctccca gaggccggtt
     3541 gcctctcgct ggtggcggtt cgggatgttc ccgggggcgg cgcgacggtc gagccgccgt
     3601 cgtccgagtc taccaccggg ccggatccgg accgttccga agccgccaga gtccatgtgc
     3661 tcgggtgtcg gtcagcgttc ccgggcgacg gtcaccgcgg acacgccgac ggcgcccgcg
     3721 gcgagcaggg gagcggcgca ggcagacagc gtcgcgccgg tcgtgacgac gtcgtcgacg
     3781 aggacgatcc agcgcccctc gagcggcctc ggcggccgct cccgcccttg gccggtgtcc
     3841 gccgccggcg tgccggccgg gcgggtgccg gacggctggg gccggagccg gaacgcaccg
     3901 cggacgttcg tcgcccgcgt cgctcgatcg agctcgaatt gggcgattgt cgcccgggcc
     3961 cgctcgagga tcggggcaca cgggagcccg agctcggccg cggccgcacg ggcgatcaac
     4021 tccgcctggt cgtagccgcg gtggcgggcg cggtcgacgt ggaccggcac gggcacgagg
     4081 acatcgccgc cggccgtcac gcgggcccag cgccgtgcga tcgccccgcc cagcggcacg
     4141 gccaggcgcg tctcgccgcc gtacttgagc tggtggaggg cgcgccggac gaggcccccg
     4201 aacggcgcgc accagtccag ctggaggatc gacggcggca cgtccgacgg caacccgatc
     4261 ggggtccccg cgggctggtc gaggcgtgcg tcgagggccg gcaagcaggt cgcgcagatc
     4321 gcggcgcctt cgcgtccgca gccggggcag cgggccggaa aggcaagatc gagcaaccgg
     4381 ttcgggagcg acgcggccgg tgacatcgcc gggatcgtct caggcggctg tgcaccgcca
     4441 cgtatcggcg agttgcttcc cgatgcacgc gaggcgcgcc gtaaccggcc ggtgatctcg
     4501 cgtgcaccgg cagtgacgcg cagtcgtcga tgatccgtcc atgaccgacg atcgcgcagg
     4561 gcgtctcgcg gccgcagtcc gccgccatct cggactccgc cagatcgacg tcgcgcgcat
     4621 ggccaaggtc gaccagaaag tggtgtcact catcgagcgc gggcagctgg agcgggtgtc
     4681 cgtgcagcgc tttcgggcgg tgtgcgccgt tctcgggatc gaaccggttc tcgatctccg
     4741 gtggcgcggt ggccaggtcg accgattgat cgatcgggga catgcccaca tcgtcgaacg
     4801 cgtgatcgcg gaactcggct tgctgggttg ggaggtcgtg ccggaattca cgttcaacca
     4861 cttcggcgag cggggctccg tggacgtgct cgcgtggcac ccggcgacgc gtgtcctgct
     4921 catcgtcgag gtgaagacgc gcctgacgga cctccaggcc atgctgatgt cgctgtcgcg
     4981 caagatccgg ctcgtgcccg gcctcgttgc ccgcgatcgg ggctgggagc gacgcgcgct
     5041 cggtcgaatc gtcgtgatcg cggacaccca cgcgaatcgg tcgaccgtgg caacgcaccg
     5101 cgccacgttc gacgcgagct tcccgagccg cacggccgct acgcgcgggt ggcttcgctc
     5161 gccgtctggc gatctggccg ggctgtggtt catcgcgatg gagggccacg gcgtccgcgg
     5221 catggaggcg ggtcggcggg tcagggcccg tagaattgcc ggtccgggcg gaagagattg
     5281 agcgatgagc cacgatgggc agcggcgaag cctggccgta cgcgggcggc tgcccggagt
     5341 cggtcggcct cttccttgcg ctcaggcgag agtcgcctgt aaggcgcgag atccgcgccc
     5401 ggtggcaagt ccgccacgct caaccccgcc tgggcgaacg cccttccgag gcgagcgtcg
     5461 cctactcgca agaaatcggg cccgggacgc gcgacaacag gcgagcgtcg cctgcgcgca
     5521 agaaatcgct ggcggcaagc tggcgccaag cggggcggag tgacgcgcgc gccgggccgg
     5581 cggagtgacg cgcgcgccgg gccggcggag tgacgcgcgc gccgggccag cggagtgacg
     5641 cgcgcgccgg gccagcggag tgacgagccg ccgcccccta cggctcgatc gcgatccgat
     5701 cccccac
//
LOCUS       DATAIW010000079         6945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_3222, whole genome shotgun sequence.
ACCESSION   DATAIW010000079 DATAIW010000000
VERSION     DATAIW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VHM48_02685"
     CDS             complement(<1..746)
                     /locus_tag="VHM48_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754338.1"
                     /translation="MTETGDIAPAAAAAPTIIHLVPHTHWDREWYEPFQVFRMRLVEL
                     VDQLLDSMEADDRMAFTLDGQVATIDDYLEIRPAGRARIERLIAAGRLAIGPWQILMD
                     EFLVSGETIVRNLELGWHAAEAFGAAMPVGYLPDMFGHVAQMPQILRRAGIRHAVVWR
                     GVPAAIDRHAFTWRSPDGSAVRAEYLVGGYGNGAYLLAIPDRLAEKVGRYADASRAFY
                     GDRSILAMYGTDHAVPSPRLARIVADANAQR"
     gene            complement(743..1918)
                     /locus_tag="VHM48_02690"
     CDS             complement(743..1918)
                     /locus_tag="VHM48_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2754339.1"
                     /translation="MTPPNYALNGNRIRVGVAGLGAVAQAVHLPLLARLDDVFEIAAL
                     ADLSPSLLARLGDRYRVAAADRVTSVEALLDRPGIDGLVILTSGSHGAAALAALGRGL
                     AVLCEKPLATTLAECDRLAASPAADRLLLGYMKVFDPAVEEARRIAVDEAATLGELKA
                     IDVTVLHPTSEAQLEFARLLPAAGDADPALLAGLGAEGDRLVEAALGSAAVAEIGPLY
                     ANLLLGSVVHDLSVIRAVTDESGPLAIDHVDVWPSAAWPPSVVVVGRLPSGVRVTIAW
                     HFLDRYPAYREEVRFHHRGGSVELTFPSPYRLHEPTILAVETGGAETRRRTILDSIEE
                     AFERELLAFAGIVRDGERPRTGIADGRTDIVTCQRIAAAWAARRGIEIGGEAAEAEG"
     gene            2087..3751
                     /locus_tag="VHM48_02695"
     CDS             2087..3751
                     /locus_tag="VHM48_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2754340.1"
                     /translation="MTDQPPIHPAAPDWRDGVVYQIYPRSFADHDGNGTGDLAGILDH
                     LDHFGPDALPVDAIWLSPIFPSPGRDLGYDVTDHSAVDPLLGREADFDRLVAACHERG
                     IKVILDLVMNHTSDEHAWFLASAASREGPYADFYLWRDPRGSDADGNPIPPNNWVSFF
                     GGPGWEWVPERQQFYYHTFLVGQPELNWRNPAVEAAQWAMVRGWLARGVDGFRLDVFN
                     AFLKDPELRDNPMIDGSSPWTRQEHRHDLDHADFADLIARFRSILDEQPGRMSVGELF
                     SGGTATSAKYTRGPHVVFDWALMESPWTAAAFGAGIDLRETAYGPDRWPTVALSNHDR
                     ERQATRLSASVGRDRDPDRDAIAKAAAVVVLAVRGTPFLYYGEEIGMTDVEIPREEIV
                     DPPALRAGPDFPWYDRSRCRTPMQWRSDPGAGFTTGRPWLRLAADAATRNVATQATDP
                     DSVLATYRRLLAFRTTAVALRTGAMERLASGAADVLAWTRTGEGQRLLVLVSFVADPR
                     RIDVGSVAAGQWLARAGTHADLPAVGGDGTLVLRPDEAVILEAVDA"
     gene            3878..4651
                     /locus_tag="VHM48_02700"
     CDS             3878..4651
                     /locus_tag="VHM48_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754341.1"
                     /translation="MPLDFLKRRNAADTPAAAATPQPGAPRSAHASISLPEEVVAQEY
                     QLKLYYAGKSSEGVRMQAGPNAVAELPSMLHGLARTEIEVVEPLPIEFSQATPVIARP
                     SEAMQWLNAHHIHSPVTRHALVVLESIDAIDLAFDTFVCTLLEGEVDTSGYPDYSAVV
                     GGVAAHWDEATGDMIVRAVVGWGGKGVRGDTDRIAAKLVAGILTNVLANQHALGLTAV
                     DRPVPAAGRGGLVCAHCGFASAHERAFYCPKCGMRLLRG"
     gene            4677..5072
                     /locus_tag="VHM48_02705"
     CDS             4677..5072
                     /locus_tag="VHM48_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2754342.1"
                     /translation="MPTYDYLCPNEHRFEVIHSVTADGPATCPICGAAPVRKAFSAPT
                     FHFKGTGWAKKDRSSASRSASRAAAASSDGGSGSDSGTASSSPAAADPSADKSGGSDA
                     KAAPDKAPSGGDKAKSSGSKTAGGPAAAD"
     gene            5074..5316
                     /locus_tag="VHM48_02710"
     CDS             5074..5316
                     /locus_tag="VHM48_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754343.1"
                     /translation="MTTSSADWISLGEAVEILAAANIRFKASTIGSWARSGKLQSIKL
                     GGRRFVRRGEVKALLVPPRRVRASDLQPSLFEDIPG"
     gene            5358..6212
                     /locus_tag="VHM48_02715"
     CDS             5358..6212
                     /locus_tag="VHM48_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2754344.1"
                     /translation="MRDRFVAAVTRVVGLPRVAIARRVLDRFAAADGALLAAGVAYNA
                     IFALIPIGLVATGVAGLVLDDARSRADMVRTIADFLPPLAGVVDEIVAGLSRASPSLS
                     IVGLVLAAWGTSRLFASLESAIAQMDVGAPRRSLIHRTARRLGSIAVLAGIVLAAIVV
                     TPTLAIAVEMNGGGPARPLLDVLLAILPPVLGGLAIAAVYKLIPLTRPTWRAIGPPTV
                     VGAVALVVLTRIFVFVAPRVFGANLVYGTLGAILVGLTWLDLVFTIVLLGAAWVRERT
                     TSFEAVVV"
     gene            complement(6268..>6945)
                     /locus_tag="VHM48_02720"
     CDS             complement(6268..>6945)
                     /locus_tag="VHM48_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2754345.1"
                     /translation="AAASSPATTHPDLAGELAAAAESPIEPVAAAPWVVEPEAEPAQA
                     ADVVPAADAAPVAGDEAPVAAESPVEIVATALPVEPATAAPTPVWPAPAPEPLAAEPP
                     RDDRIQQPTWQIFAPDATPATDRPLDGAVPPAAPVQPPFAASADPQWPVRPDQADSPA
                     MALLANRANRSPSDALWAASAQEVLARPASVPVAGAATGVQPCSSCGLSLSATARFCR
                     RCGTRQG"
BASE COUNT          935 a         2519 c         2502 g          989 t
ORIGIN      
        1 cgctgggcat tggcgtccgc gacgatccgg gccaggcgcg gggaggggac cgcgtggtcg
       61 gtgccgtaca tcgcgaggat cgagcgatcg ccgtagaacg cccggctcgc gtcggcgtaa
      121 cggccgacct tctcggcaag ccgatcgggg atcgcgagca ggtaggcgcc gttgccgtag
      181 ccgccgacga ggtactccgc gcggaccgcc gagccgtccg gcgagcgcca ggtgaaggcg
      241 tgccggtcaa tcgcggcggg cacgccccgc cagacgacgg cgtggcggat gccggctcgg
      301 cgcaggatct gcggcatctg ggcgacgtgg ccgaacatgt cgggcaggta gccgaccggc
      361 atggccgccc cgaacgcctc ggcggcgtgc cagccgagct cgaggttgcg gacgatcgtc
      421 tcgccggaca cgaggaactc atccatgagg atctgccagg gcccgatcgc gagacggccc
      481 gcagcgatca ggcgctcgat ccgggcccgg ccggccgggc ggatctcgag gtagtcgtcg
      541 atcgtggcga cctggccgtc cagcgtgaag gccatccggt cgtccgcctc catcgaatcg
      601 aggagctggt cgacgagctc cacgagacgc atccggaaca cctggaacgg ctcgtaccac
      661 tcacggtccc agtgggtgtg gggcacgagg tggatgatcg tcggcgcggc ggcggcggcc
      721 ggcgcgatgt ccccggtctc ggtcatccct cggcctccgc ggcttcgccg ccgatctcga
      781 tcccgcgccg ggccgcccac gctgccgcga tgcgctgaca ggtcacgatg tccgtccggc
      841 cgtcggcgat ccccgtccgc ggacgttcac catcacggac gatgccggcg aaagcgagca
      901 gctcgcgctc gaacgcctcc tcgatcgagt cgaggatcgt tcgccggcgc gtctcggctc
      961 cgcccgtttc gaccgcgagg atcgtcggct cgtggagccg gtacggcgat gggaacgtca
     1021 gctcgaccga tccgccgcgg tggtggaacc ggacctcctc ccggtacgcc ggatagcggt
     1081 cgaggaaatg ccaggcgatc gtcacccgga cgcccgacgg cagccgcccg acgaccacga
     1141 cggacggtgg ccaggcagcg ctcggccaga cgtcgacatg gtcgatggcg agcggtccgc
     1201 tctcgtcggt gacggcccgg atcaccgaca ggtcgtggac gacgctgccg aggagcaggt
     1261 tggcgtagag cggcccgatc tcggcgaccg cggcggatcc cagcgccgcc tcgacgagcc
     1321 ggtctccctc ggcgccgagt ccggcgagca gcgccgggtc ggcgtcgccg gccgccggca
     1381 ggaggcgggc gaactcgagc tgggcctcgc tcgtcgggtg gaggacggtc acgtcgatgg
     1441 ccttcagctc gccaagcgtc gcggcctcgt cgaccgcgat ccgacgggcc tcctcgaccg
     1501 ccgggtcgaa gaccttcatg tagccgagca ggagccgatc ggctgccggc gaagcggcga
     1561 ggcgatcgca ttccgcgagc gtcgtcgcga gcggtttctc gcacaggacg gcgaggccgc
     1621 gtccgagggc ggcaagggcc gccgcgccgt gcgagccgga ggtcaggatg acgagcccgt
     1681 cgatgccggg tcggtccagc agcgcctcga ccgacgtgac ccgatcggcc gcggccacgc
     1741 ggtagcgatc gccgagccgc gccagcaggg acggcgaaag gtcggccagg gcggcgatct
     1801 cgaacacatc gtcgagacgg gccaggagcg ggaggtggac ggcctgggcg accgctccga
     1861 gcccggccac gccgacgcgg atgcggtttc cgttgagcgc gtagttgggt ggggtcatgg
     1921 cgcccgagta taggagggcg ggcagcacga ccgggctgca agtcgtcgcg cgaccgcgac
     1981 cggtgcccgt ttcggtcgcc cgccgcccga accggcgaag cagccgtcgc ccgacccggc
     2041 gacagtcgtt tgacgccccg ccgggccgct ctgcagactt ccggccatga ccgatcagcc
     2101 gccgatccac cctgcagccc ctgactggcg cgacggcgtc gtctaccaga tctacccgcg
     2161 cagcttcgcc gaccacgatg ggaacggcac cggcgacctc gccgggatcc tcgatcacct
     2221 cgaccatttc ggaccggacg ccctgccggt cgacgcgatc tggctgtcgc cgatcttccc
     2281 gtcgcccggt cgcgacctcg gctacgacgt caccgatcac agtgccgtcg atccgctcct
     2341 gggccgcgag gccgacttcg accgcctcgt cgcggcctgc cacgagcggg ggatcaaggt
     2401 gatcctcgat ctcgtgatga accacacgag cgatgagcac gcctggtttc tcgcctcggc
     2461 ggcctcccgc gagggcccgt atgccgactt ctacctgtgg cgcgaccctc gtggcagcga
     2521 tgccgacggc aatccgatcc cgccgaacaa ctgggtctcg ttcttcggcg gtccgggctg
     2581 ggaatgggtg ccggagcggc agcagttcta ctaccacacg ttcctcgtcg gtcagcccga
     2641 gctgaactgg cggaaccccg ccgtcgaggc cgcccagtgg gcgatggtcc ggggctggct
     2701 cgcccgcggc gtcgacggct tccggctgga cgtcttcaac gccttcctca aggaccccga
     2761 gctgcgcgac aacccgatga tcgacggctc gtcgccgtgg acccgccagg agcatcgcca
     2821 cgatctcgat cacgccgact tcgccgacct gatcgcccgg ttccggtcga tcctcgacga
     2881 gcagcccggc cggatgtcgg tcggtgagct cttctccggc gggaccgcga cgtccgcgaa
     2941 gtacacccgg ggtccccacg tcgtcttcga ctgggcgctc atggaatcgc cgtggacggc
     3001 cgccgcattc ggggccggga tcgacctccg tgagacggcc tacggccccg atcgctggcc
     3061 gaccgttgcc ctctcgaacc atgatcggga gcgccaggcg acccgcctgt cggcctcggt
     3121 cgggcgcgac cgtgatccgg atcgcgacgc gatcgcgaag gcggcggccg tcgtcgtgct
     3181 cgccgtccgc ggtaccccgt tcctctatta cggcgaggag atcgggatga ccgacgtgga
     3241 gatcccgcgc gaggagatcg tcgatccgcc cgcgctccgg gccgggcccg acttcccgtg
     3301 gtacgaccga tcgcgctgcc ggacgccgat gcagtggcgt tcggatccgg gcgccggctt
     3361 cacgaccggc cggccctggc tgcgcctcgc cgccgacgcg gcgacgcgca acgtcgcgac
     3421 ccaggcgacg gacccggatt cggttctcgc gacctaccgc cggttgctcg ccttccggac
     3481 gacggcggtg gctctccgga ccggggcgat ggaacgcctc gccagcggcg ccgccgacgt
     3541 gctggcctgg acgcggacgg gcgagggcca gcgactcctc gtcctcgtca gcttcgtcgc
     3601 cgacccgcgt cgcatcgacg ttggcagtgt cgccgccggg cagtggctgg cgcgggcagg
     3661 gacgcacgcg gacctgcccg cggtcggcgg cgacggtacg ctggtcctgc gcccggacga
     3721 ggccgtcatc ctcgaggccg tcgacgcctg agcgcgacga ccgaccccgg ggacgacccg
     3781 ccatccggcc cgatcccgtg ccgaacgtac tctgatcggc gcaggttcgc cgctgctacc
     3841 atgagcgccg ttcggtcctg accaacggag cctcgtcgtg ccgctcgatt tcctcaagcg
     3901 ccgcaatgcc gccgacacgc ccgccgcggc ggcgacaccg cagcccggcg cgccccggag
     3961 cgctcacgcc tcgatctcgc ttcccgagga ggtcgtcgcc caggagtacc agctcaagct
     4021 gtactacgcg ggcaagagca gcgagggcgt ccggatgcag gccggtccga acgccgtggc
     4081 cgagctgccg tcgatgctcc acgggcttgc caggaccgag atcgaggtcg tcgagccgct
     4141 gccgatcgag ttcagccagg cgacgccggt catcgcccgc ccgtccgagg cgatgcaatg
     4201 gctgaacgcc catcacatcc actcgccggt cacccgccac gccctcgtcg tgctcgaatc
     4261 gatcgacgcg atcgacctcg cgttcgacac cttcgtctgc acgctcctcg agggcgaggt
     4321 ggacacgagc ggctatccgg actactcggc cgtcgtcggt ggagtcgcgg cgcactggga
     4381 cgaggcgacc ggcgacatga tcgtccgagc cgtcgtcggc tggggtggca agggcgtccg
     4441 cggcgacacc gatcggattg cggcgaagct cgtggcgggg atcctcacca acgtcctcgc
     4501 caaccagcac gcattgggtc tcaccgccgt cgaccggccc gtgcccgccg cgggtcgcgg
     4561 tggtctcgtc tgcgcgcatt gcggcttcgc gtcggcccac gagcgggcgt tctactgccc
     4621 gaagtgcggc atgcgcctcc tccgcggctg accgaccggt ccgcccgaca ccgccgatgc
     4681 cgacctacga ctacctctgc ccgaacgagc atcgcttcga ggtcatccac tccgtcacgg
     4741 ccgacggacc ggcgacctgc ccgatctgcg gcgccgcgcc ggtccgcaag gcgttctccg
     4801 ccccgacgtt ccacttcaag ggaacgggct gggcgaagaa ggatcgcagc tccgcctcgc
     4861 gctctgcgtc gagggcggcg gcagcgtcgt cggatggtgg ctcgggctcc gattcgggga
     4921 ccgcgtccag ctcgccggcg gcggccgatc cgagtgccga caagagcggg ggcagcgacg
     4981 cgaaggcggc tcccgacaag gcgccgtcgg gtggcgacaa agcgaagtcc tcgggttcga
     5041 agacggcggg cggacccgcg gcggccgact gacatgacga cctcttctgc cgactggatc
     5101 agcctcggcg aggcggtcga gatcctcgcg gccgccaaca tccggttcaa ggcatccacg
     5161 atcggcagct gggcccggag cggcaagctc cagagcatca agctcggcgg tcgccgcttc
     5221 gttcgccgcg gtgaggtgaa ggcgcttctc gttccgccgc gacgcgtccg cgcgagtgac
     5281 ctccagccga gcctgttcga ggacatcccg ggctgaccga cgtcgggcgc gacccgcgtc
     5341 ctgctcgtcg cgcgatcgtg cgcgaccggt tcgtcgccgc cgtgacgcgc gtcgtcgggt
     5401 tgcctcgagt cgccatcgct cgccgcgtcc tcgaccggtt cgctgccgcg gacggtgcat
     5461 tgctcgccgc cggtgtggcc tacaacgcga tcttcgccct gatcccgatc ggcctcgttg
     5521 cgaccggcgt cgccgggctg gtcctcgacg acgcccgcag ccgggccgac atggtccgga
     5581 cgatcgccga cttcctgccg ccgctggctg gagtcgtcga cgagatcgtc gccgggctgt
     5641 cgcgcgcgtc accatcgctg tcgatcgtcg ggctcgtgct tgccgcgtgg gggacgtcgc
     5701 ggctgttcgc ctcactcgaa tcggcgatcg cccagatgga cgtcggagct ccgcggcgca
     5761 gcctgatcca ccggacggcc cggcgcctcg gctcgatcgc ggtcctcgcc ggaatcgtcc
     5821 tggccgcgat cgtcgtgacc cccaccctgg cgatcgcggt cgagatgaac gggggtggcc
     5881 cggcccggcc gctcctcgac gtcctcctcg cgatcctgcc gcccgttctg ggcggactcg
     5941 ccatcgcagc ggtgtacaag ctgatcccgc tgacccgccc gacctggcgc gcgatcggcc
     6001 caccgaccgt cgtcggcgcc gtcgctctcg tggtcctgac gcggatcttc gtgttcgtcg
     6061 cgccccgcgt gttcggtgcg aacctggtct acgggacgct tggggcgatc ctggtcgggc
     6121 tgacgtggct cgacctggtg ttcacgatcg tcctgctcgg cgccgcgtgg gtccgggaac
     6181 ggacgacgtc gttcgaggct gtggtcgtct agcgccgaca gagcgcgacc cggagctgcg
     6241 aggctcggtc gcggtggcga ccgacggtca gccctggcgc gttccgcagc ggcggcagaa
     6301 ccgggccgtg gccgacagcg acagcccgca gctcgagcac ggctggactc ccgtcgcggc
     6361 gccggcgacc ggcacgctgg ccgggcgagc gaggacctcc tgggccgagg cggcccacag
     6421 ggcgtcactc ggagagcggt tggcacggtt ggcgaggagg gccatcgccg gcgaatcggc
     6481 ctgatcgggc cggaccggcc actgcgggtc ggcggatgcg gcgaaaggag gctgaacggg
     6541 agcggccggc gggacggcac catcgagcgg gcggtcggtg gccggcgtcg cgtccggcgc
     6601 gaagatctgc caggtcggct gctggatgcg gtcgtcgcgc ggcggctccg cggcgagggg
     6661 ctcgggcgcc ggcgcgggcc agaccggggt cggcgcggcg gtggcaggct cgactggcag
     6721 cgcggtcgcg acaatctcga ccggcgactc ggcggcgacg ggcgcctcgt ctccggcgac
     6781 aggggcagcg tcggcagccg gcacgacatc cgcggcctgg gcgggctcgg cctctggctc
     6841 gaccacccac ggcgccgcgg cgaccggctc gatcggcgat tcggccgcag ccgccagctc
     6901 gccggccagg tccggatggg tcgtggccgg cgagctggcg gctgc
//
LOCUS       DATAIW010000080        16443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_33362, whole genome shotgun sequence.
ACCESSION   DATAIW010000080 DATAIW010000000
VERSION     DATAIW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16443
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33362"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..624
                     /locus_tag="VHM48_02725"
     CDS             172..624
                     /locus_tag="VHM48_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754346.1"
                     /translation="MIRPLLAYAAVVAIGVVAWVALGGSRADIVASIVLLGLVTTPGF
                     VLSVELVRRFAPTSWPSRALVGAASWAGWWLVVAVIATVLSRVVLLPGELTRVDAIAA
                     AAGALFSVLAFDGRKRRPGMVLIGLALAVTALIVVGAVWMAGRWGGAV"
     gene            complement(664..1347)
                     /locus_tag="VHM48_02730"
     CDS             complement(664..1347)
                     /locus_tag="VHM48_02730"
                     /inference="COORDINATES: protein motif:HMM:NF036876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEX2754347.1"
                     /translation="MPRSPSPETNAAAARIDVLEGPDAIVAVVSRIAERGGRIRTVAT
                     IRALDTDPPQSEIEVEVEGLTAEELLDALEGLPEIQAIRRTRALGSIFGKRVIVVGGG
                     AQVAQVALGAVSEADRHNLRGERISVDTIPLVGEANIAAAVRAVADLPRARLLVLAGS
                     IMGGDISNAADELRAVGVPIIALNMVGSITEHVDLVVSDPVQAGTMAVMAIADTASFD
                     LVRQRGRRY"
     gene            complement(1366..1629)
                     /locus_tag="VHM48_02735"
     CDS             complement(1366..1629)
                     /locus_tag="VHM48_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2754348.1"
                     /translation="MTVWFTVKLDDRPGALARMATALAERGVNIAGIVGIAEDTDGEL
                     MLETSNAAETRAAFDAAGIAYEAHDADGAADPGRMSIADIVGR"
     gene            complement(1729..2454)
                     /locus_tag="VHM48_02740"
     CDS             complement(1729..2454)
                     /locus_tag="VHM48_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754349.1"
                     /translation="MVCRSVPRPIARAAIGAVLASLALAASWFAPPVPAAAALSTWTG
                     GIDLYRTGVFTTQKTWLWCTAADVQIIRNIVDHQTDHTRSHQQVYFDYMRAHNRYSIP
                     VADGVDPGGWAAGLDHYVDTRYHLVASASFSSALRSAVTNLRRTNLPVGITVAHGNHA
                     WVLTGFTATADPARTTSFTVTTVRVTGPLWGLQSRTYGYDMRPDTRLTIAQFRTFFTA
                     WHYARIRMSWEGRWVSIQPIPPG"
     gene            2535..2912
                     /locus_tag="VHM48_02745"
     CDS             2535..2912
                     /locus_tag="VHM48_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX2754350.1"
                     /translation="MPASTELSVQIFGHDRDQGTRAAVRFFKERRVQIHQVDLTRKPI
                     ARGELRRFVDKLGARDLLDETSRAYREGGLGYMRLDDAEIVERLLATPALLRLPLVRF
                     ENDVAVGRDETGWKGWFKPGGGA"
     gene            complement(2890..3084)
                     /locus_tag="VHM48_02750"
     CDS             complement(2890..3084)
                     /locus_tag="VHM48_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754351.1"
                     /translation="MIRSRTILAIILVAIGLVWIGQGTGLLQGTSFMVGDGRWTLIGA
                     VCVVAGIALGWLEIRRRRQA"
     gene            3117..4055
                     /locus_tag="VHM48_02755"
     CDS             3117..4055
                     /locus_tag="VHM48_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2754352.1"
                     /translation="MNDRTAPAGSTDPGAEPEPTPGADQGHAAKAPAPGPPTPGVAAP
                     GAARPAEPERRSGRVTMRGHAARRIGKSTETEDEKFLERRPKQPGRPEFIDSDPWRAL
                     RILSEFVEGFDALALVGPAVTVFGSARVKPGSVDYELARGIARRLAEAGFAIITGGGP
                     GIMEAANRGCREGGGLSIGCNIELPHEQGLNAYVDLGVEFKYFFARKTMFVKYADAFV
                     IMPGGFGTLDELFESLTLIQTGKIRDFPVVLVGSGYWQGMLDWMRTVQIPAGAVSEDD
                     LNLLKLTDDPDEVCEIISAYVAKAHPEDFAIEDEIR"
     gene            complement(4539..5039)
                     /locus_tag="VHM48_02760"
     CDS             complement(4539..5039)
                     /locus_tag="VHM48_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2754353.1"
                     /translation="MTIDRPTFPIPAAFPIELTAPGVADGGPVDAGPLADLPEGSMRR
                     ISHGDLDVLIAHTPAGIVAVDDRCPHMAAPLSIGTLEGCVVACPLHEGRFDLCSGEVV
                     QMPTTGGLDPDGSYRPTWSPAGKDPKVDPPGKKAEARRLTRVRRFRYYPVRIVDGRVE
                     VTLPEG"
     gene            complement(5117..6685)
                     /locus_tag="VHM48_02765"
     CDS             complement(5117..6685)
                     /locus_tag="VHM48_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2754354.1"
                     /translation="MLGMFLGALDQTIVGPVLPKIVTDLNGADLYTWVVTIYLLTSTV
                     SVPIYGKLSDLYGRKPLLIAGIVLFLAGSALSGLSQTMWQLILFRGIQGLGAGSLFPI
                     ALAVIGDLFTPAERGRYQGLFGGVFGLAFIIGPFLGGYLTDHFGWHWIFFVNLPIGLI
                     ALFVISRLLPNVHGKVTKISIDYLGVATLVLGLVPILIGLTIAETGGFGDPYVWIWSA
                     VGAVFLVIFVLVERRAAEPVIPLHLFRNRTFSASMVAIFFATFGFGAAIIFLPLYFQV
                     VEGANATESGYRLLPFLAGLIFSSILSGLVVSRTGKYKPTLIIGIVVLVIGLGLMTQL
                     RAGTDDLTLAIWMVIAGLGVGPTFAVFTIVVQNSVPFHELGAATSDLTLFRQIGTTVG
                     ITMAFTLFRINLSWDLLRNQIIDAGAPAALVPATPPAGFDLGTLTSVGGSSPLDFVSQ
                     IPAQFQAAFLNGFHGAFTIAIADSLWLGVGASALAVVAVLLLHEIPLRRTTGAEVPVS
                     TPEPGGQRGQPVLD"
     gene            complement(6848..7363)
                     /locus_tag="VHM48_02770"
     CDS             complement(6848..7363)
                     /locus_tag="VHM48_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2754355.1"
                     /translation="MRTTDALDAPASTPASPPQLVDSVVDELHAMIGSLRCAGTGRMV
                     KAGISMTHLHILWVLEHHGDLPMSRLADLLDVSFSNATGLIDRMEERGLVERDRVPDD
                     RRVVIVRASAEGARQRDEIEALKQDRLRSILERLSQEQLTRILGAIGDLRTAVIDEIG
                     TDHLHPHARNV"
     gene            7450..8421
                     /locus_tag="VHM48_02775"
     CDS             7450..8421
                     /locus_tag="VHM48_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2754356.1"
                     /translation="MRFALMIEPQQGLTYDEQLAIVRRAESVGFESFFRSDHYQSFPG
                     PSGEPTTDAWAVLAGLARETSTIGLGTLVSPVTFRPVGNLAKVVTTVDEMSDGRVEFG
                     LGAGWNELEHRELGLPFPDIKDRADLMEEQFAVLRGLWGEPDGWSFDGAHVQIADASF
                     HPKPVARTGRPTFANGSARPRLLAGGEGSPRSMRIAARYADEFNLSSSSPEKAATKFA
                     ELRAACEAIGRDPATMGRSVMAGVLVGRDAAELDGRKAALLAAFGNEAGGDDWFDARE
                     PRWVLGTPEQARAMVSRFEAAGAERLVLQDFLPRDLAMVDLMAEVLF"
     gene            complement(8511..9710)
                     /locus_tag="VHM48_02780"
     CDS             complement(8511..9710)
                     /locus_tag="VHM48_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754357.1"
                     /translation="MTTFDPHAPLAPAPDPWAELPQPSARSGPPFHMTDMIAAEPFLA
                     ERILDRLADPQGPAGRLAGAIGQAASSGASIVVTGCGTSEHAAHAVVEILREGLRAGG
                     LPSGPSTILVAQAFELALDPPTGGLVIGISHEGGTVATIRALEASAAAGARTALITGS
                     AESPAAAAVASPDMVVATVEMDHGWCHTVGYLSPIVAAAAIADELADRTTDGGLVRDL
                     LISGSADEAGAEAIAAILAGCTTLITLASGADRPAARELALKIEEAAWLPTTMRDLET
                     FLHGHLPAMDPTTGLVLLLTDRDARAARVGRARQALAAAKAVGIRSAAIVARDVHGQL
                     DADLTPAGRIRVDEAKALPAPLAALLGTATSLQLLTERIARVRGTNPDLIRRDDPIYL
                     AAADAAG"
     gene            9803..10708
                     /locus_tag="VHM48_02785"
     CDS             9803..10708
                     /locus_tag="VHM48_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2754358.1"
                     /translation="MQVGLMAPQGWKGGYDGWDAAGAWARTVELATQAEELGFESLWV
                     FDHFHTVPVPTDEITFESFSVLSALAMTTRRVRLGHMVVCTGFRNPAHTAKLSSTIDV
                     ISGGRFELGIGAGWKKDEWEAYGYGFPPIGERMDAFGDDLEVITRMFAPGHASYEGAH
                     AHVRDAINVPKGIQQPRIPIIVGGNGEKRTAGYAIKFADELNYVFLDAPEIAERMISV
                     RERCDAEGRDPATLRFSLYVRDEDVRDAGQARVDLLAGYAAIGLDRIVCFPTRWSPTA
                     DAQAAFAADVRSAGIELEVGEPVSV"
     gene            complement(10698..11159)
                     /locus_tag="VHM48_02790"
     CDS             complement(10698..11159)
                     /locus_tag="VHM48_02790"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX2754359.1"
                     /translation="MGLRRIRGRARLQPVRERARGPWRVVVSEASMAPTIQPGDWLLV
                     DPTTARWPRRGAIVVFREPDSDLLAIKRVAAGPGARVDLDEGHLVLAADEAWLLSDAS
                     DADLLAAGAGQPIDSRRYGPVPVEALVGRVWFRYGPIDRIGPVRRPSAVRR"
     gene            complement(11240..11671)
                     /gene="sodN"
                     /locus_tag="VHM48_02795"
     CDS             complement(11240..11671)
                     /gene="sodN"
                     /locus_tag="VHM48_02795"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX2754360.1"
                     /translation="MFRVRTFLAPSTTVHAHCDLPCGVYDPEQARIEAESVYRIIEKM
                     AANSDPTFTARAIGIKEKQADLAKHHLDVLWHDYFKPEHLEKVPNLHELFWNATKQVS
                     KVKASTDLADAQKLLDMIDEIDAAWKATGGEGKTRVAGRPA"
     gene            12299..13129
                     /locus_tag="VHM48_02800"
     CDS             12299..13129
                     /locus_tag="VHM48_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX2754361.1"
                     /translation="MEIVIIALLVLLIAAVLVRPVVTVATIHDYQRGLRYRNGRLVGL
                     LDPGTHIAIRPFTEIHVMDGRPTSITVPGQEILTADGVALRVSLTGRYVVADPVAAVT
                     NDQSYVTALYTALHGGLREALAGRTADEVIADRAAIGPAVGGAVASELARIGVELLGI
                     DVRDVMVPSELKRAFAGIVAARREGEAAVERARGETAALRSLANAGRMIEDNPGLLQL
                     RILQQLGGSSGNTIMLGLPGSDGRSEGGSAPPPTAAAASTRSRPSTRSRPPARRSAQD
                     "
     gene            13151..14443
                     /locus_tag="VHM48_02805"
     CDS             13151..14443
                     /locus_tag="VHM48_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX2754362.1"
                     /translation="MTERGLPLADGAPACPFVAFEDDRDERASVPDHRHRCYADQRPA
                     PRALAHQEAYCLSSAFPVCPTFQDWARREAARSRDGAPTAPSASAGMNRAATGAVADA
                     DAADAASAGAAGVGGAAGIAGAAGAGASGPAAGLADTSRDVRRTVDVDDSHADDLVRR
                     NPPRDWAAPPPWLASNEPRGRGDAEPPAFLGHRVTEPGQGLAGSPADRLAGGPPPRRD
                     ESDDVNWSSESRSAAAIGAGLAGGGAEPGPRRRSVPASDGEQDDDGEARPARRPRAYA
                     QHLGGPDGPDWEQPRRYEAYPTIKARVGLPAVPRIVGVAALVAVLALALFFLPGMLNI
                     GGGTATPGGSPGASLRPGPSVSLAPTEQPAATPIIYTIKHGDVLSKIAAAHGITLEQL
                     MAANPTIKNPNKISEGQQITIPLPSAAPPDTIDGSAAP"
     gene            complement(14856..15185)
                     /locus_tag="VHM48_02810"
     CDS             complement(14856..15185)
                     /locus_tag="VHM48_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2754363.1"
                     /translation="MGAVDTMDTFFELLNAGDREAAVQLMAENAEMRIHVGDSAQTLR
                     GVERVGGWFLRGDAGLRMIPGDVRDTGNTYEADVLVVRPGAPSLHLDATFRVEAGKIT
                     SINLAPR"
     gene            15215..>16443
                     /locus_tag="VHM48_02815"
     CDS             15215..>16443
                     /locus_tag="VHM48_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2754364.1"
                     /translation="MPSDLAGQWALDPAVAFLNHGSFGACPRPVLEAQRAWRDRMEAQ
                     PVQFFARDLPGLLAAARRDLGAFVGADRNDLAFVANATGAVNAVVRSLRFAPGDELLT
                     DDHEYNATINVLRHVAERDGARVVVATIPFPVASEDAVADAILGAATDRTRLALVSHV
                     TSPTALVFPIERIVAGLAERGVETLVDGAHAPGMLALDLERIGAAWYTGNLHKWVCAP
                     KGAAFLHARRDRQPGIRPNTISHGANEIIGADRVDGRTRYRAEFDWQGTLDPTAWLAV
                     SEALRFVGGLVDGGWPEVMARNRAMTLRTRVAMTAVLGLDDAVPAPESMLGSLVALPL
                     PAFGPLAARGSGSSPLDTDPLQQRLIEEFQVEVPIYPWPVPAAESPQASRRLIRVSSA
                     LHNGPDDVECLVAALGA"
BASE COUNT         2382 a         5706 c         6016 g         2339 t
ORIGIN      
        1 gcgcgaccgc tgggccggct ggaagcggga gccgttcacg agcgacagcc ggagccacgt
       61 ttccgtctgg gagaagcccg cgccgtagac ggttctcctt gcggcggcgg ctagactcgg
      121 cccatgacga cgggagcgag gacctggggc gggaggctgg tcggcgatcg catgatccga
      181 ccactcctgg cgtacgcggc agtcgtcgcg atcggcgtgg tggcctgggt cgcactcggt
      241 ggatccaggg cggacatcgt cgcgtcgatc gtcctgctcg gcctcgtcac gacacccggg
      301 tttgtcctca gcgtggagct cgtccgccgg ttcgcgccga cctcgtggcc gagccgagcg
      361 ctcgtcggcg cggcgtcctg ggcggggtgg tggctggtgg tcgcggttat cgcgacggtg
      421 ctcagtcgcg tcgtcctgct ccccggcgag ctgacgcggg tcgacgcgat cgcggcagcg
      481 gccggcgccc tgttctcggt cctggccttc gatgggcgaa agcggcgacc ggggatggtg
      541 ctcatcgggt tggcgctcgc ggtgacggcg ctcatcgtcg tcggcgccgt gtggatggcc
      601 ggacgctggg gcggcgcggt ctgatccctc gccccttcgt cggcgacgag tcggccccat
      661 gcctcagtag cggcggcccc gctggcggac aaggtcgaag gacgccgtat ccgcgatcgc
      721 catcacggcc atcgtgccgg cctggacggg gtcgctgacg acgaggtcga cgtgctcggt
      781 gatcgagccg accatgttca gggcgatgat cgggacaccg accgcccgga gctcgtcggc
      841 ggcgttgctg atgtcgccgc ccatgatcga gcctgcgagg acgagcaggc gtgcccgcgg
      901 caggtcggcc accgctcgca ccgcggcagc gatgttcgcc tcgccgacca gcgggatcgt
      961 gtcgacgctg atccgctcgc cgcggaggtt gtggcgatcg gcctcgctca cggcaccgag
     1021 cgcgacctgg gcgacctgcg ccccgccacc gacgacgatc acccgcttgc cgaagatgct
     1081 gccgagggcc cgcgtccggc ggatcgcctg gatctcgggc aggccctcga gcgcatcgag
     1141 cagctcctcg gcggtcagcc cctcgacctc gacctcgatc tccgactggg gcgggtcggt
     1201 gtcgagcgcc cggatcgtcg ccaccgtccg gatccggcca ccgcgttcgg cgatccgcga
     1261 gacgaccgcg acgatcgcat cgggcccctc gagcacgtcg atccgtgccg cggcggcgtt
     1321 ggtctcgggc gagggactgc gcggcatgtc gggagggcag ccggcctagc ggccgacgat
     1381 gtccgcgatc gacatccgac ccggatcggc cgcgccgtcg gcgtcgtggg cctcgtaggc
     1441 gatcccggcc gcgtcgaagg cggcgcgcgt ctcggcggcg ttgctcgtct cgagcatcag
     1501 ctcgccgtcg gtgtcctcgg cgatcccgac gattcccgcg atattgacgc cgcgttccgc
     1561 cagcgcggtc gccatccggg cgagcgcacc cggccgatcg tcgagcttga ccgtgaacca
     1621 gaccgtcacg cgggcacctc ctgtccggcc gatggtaccc gcccgaccgg cgccgggcat
     1681 cgaccaagcg gggcccggtg ccttccgcgg cgtgctcggc tcggacagtc agccgggcgg
     1741 gatcggctgg atcgagaccc agcggccctc ccacgacatc cggatccggg cgtagtgcca
     1801 ggccgtgaag aaggttcgaa actgggcgat cgtgagcctc gtgtcgggcc gcatgtcgta
     1861 gccgtaggtc ctgctctgga gcccccacag cggcccggtc acccggacgg tcgtgaccgt
     1921 gaagctggtg gtcctcgccg ggtcggcggt ggcggtgaac cccgtcagga cccacgcgtg
     1981 gttgccgtgc gcgacggtga tcccgaccgg caggttcgtc cggcgcaggt tcgtgacggc
     2041 cgaccggagc gctgaactga agctcgcact tgcgacgagg tggtaacgcg tgtcgacgta
     2101 gtggtcgagg cccgccgccc agccgcccgg gtcgacgccg tccgcgaccg ggatcgagta
     2161 ccggttgtgg gcgcgcatgt agtcgaaata gacctgctgg tgggaccgcg tatggtcggt
     2221 ctggtggtcg acgatgttgc ggatgatctg gacgtccgcc gccgtgcacc agagccaggt
     2281 cttctgggtc gtgaatacac cggtccggta gaggtcgatc ccgccggtcc acgtcgacag
     2341 cgccgcggcg gccgggacgg gcggcgcgaa ccaggaggcg gcaagggcga gggaggccag
     2401 gacggcgccg atggccgcgc gggcgatcgg ccggggcacg ctgcgacaga ccatggcgcc
     2461 atggtgcccc cgctcgcgga ttcgcgcccg caagccggcg cgctccgctc gatgaccgat
     2521 acgatctgac cgtcatgccc gcctcgaccg agctcagcgt ccagatcttc ggccacgacc
     2581 gcgaccaggg cacccgtgct gcggtccggt tcttcaagga gcgccgcgtc cagatccacc
     2641 aggtcgacct gacccgcaag ccgatcgccc gcggcgagct gcgccggttc gtcgacaagc
     2701 tcggcgcgcg ggacctcctc gatgagacgt cgcgggccta cagggaaggc ggcctcggct
     2761 acatgcgcct cgacgatgcc gagatcgttg agcgcctcct cgcgacgccg gccctcctcc
     2821 gcctgccgct cgtccgattc gagaatgatg tggccgtggg ccgggacgag accggctgga
     2881 agggctggtt caagcctggc ggcggcgcct gatctcgagc cagccgaggg cgatcccggc
     2941 gacgacgcac accgcgccga tcagggtcca gcggccgtcg ccgaccatga agctcgtgcc
     3001 ttgaaggagt cccgtgccct ggccgatcca gaccaggccg atcgcaacga gaatgatcgc
     3061 cagaatggtg cgtgaccgaa tcatcgagtc tcctgtctca aggggatcct aatcccatga
     3121 acgaccgaac agcccccgcc ggttcaaccg atcccggcgc cgagcccgag ccgacccccg
     3181 gggccgatca gggccacgcc gccaaggcac ccgccccggg cccacctacc cctggcgtgg
     3241 ccgccccggg cgccgccagg cccgcggagc cggagcgccg ctccggccgg gtcacgatgc
     3301 gcggccatgc cgcccggcgg atcggcaaat cgaccgagac ggaggacgag aagttcctcg
     3361 agcgccgccc gaagcagccc ggccggcccg agttcatcga cagcgatccg tggcgcgccc
     3421 tgcggatcct cagcgagttc gtcgaggggt tcgatgcgct cgccctggtc ggtccggcgg
     3481 tcacggtctt cgggtccgcc cgggtcaagc ccggatcggt cgactacgag ctcgcccgcg
     3541 gcatcgcccg ccggctggcc gaagcgggct tcgcgatcat caccggcggc gggcccggga
     3601 tcatggaggc cgcgaaccgc gggtgtcgcg agggcggcgg cctgtcgatc ggctgcaaca
     3661 tcgagttgcc ccacgagcag gggctcaacg cctatgtcga tctcggggtc gagttcaagt
     3721 atttcttcgc ccgcaagacg atgttcgtga aatacgccga cgcgtttgtc atcatgcccg
     3781 gcgggttcgg gacgctcgac gagctgttcg agagcctgac cctcatccag acgggcaaga
     3841 tccgcgactt cccggtggtc ctcgtcgggt cgggctactg gcagggcatg ctcgactgga
     3901 tgcggaccgt ccagatcccg gccggcgccg tctccgagga cgacctgaat ctgctcaagc
     3961 tcaccgacga ccccgacgag gtctgcgaga tcatcagcgc ctacgtcgcg aaggcgcatc
     4021 cggaggattt cgcgatcgag gacgagatcc gctaggcgcg ggccggaaac gggccgtctc
     4081 gaggcccgat tcacgcccca gttgcaccag cgacgcgctg gaccagtgcc ggcctcgatc
     4141 ccgcccggga ttcacctcga gcgtgccaga ggggccgatt cgaggccgat ccggacgatc
     4201 agcccattcc catggtcgct cgtgcacgcc cgtggagagt cgccgggctc gccggggccg
     4261 ccgtgggatt ccttgcatgt aggcgactga cgcctattgc gcgcccatct ggacggcgaa
     4321 ccgagggtcg ccacgctcgg agcccaggcg cgcggcgtcc gcgtaggtga gcgtcgcctg
     4381 ggcgccagag atccgggcgc cgatggccca caacaggcga gcgtcgcctc ggcgcaaagg
     4441 atccgggcgc cggccgccca tgacaggtga gcgtcgcctg ggcgccaggg attcgacgcg
     4501 gccaggaccg acggatcgga ggcgccgcgt cagcccggtc acccctccgg cagcgtgacc
     4561 tcgacccggc cgtccacgat ccgaacgggg tagtagcgga atcgccggac acgggtcagg
     4621 cggcgggcct cggccttctt gccgggcggg tcgaccttgg ggtccttgcc ggccggcgac
     4681 catgtcggcc ggtaggagcc gtcgggatcg agaccgccgg tcgtcggcat ctggacgacc
     4741 tcgcccgaac agaggtcgaa ccggccttcg tggagcgggc aggcgaccac gcagccctcg
     4801 agcgtgccga ttgacagcgg cgccgccatg tgcgggcagc gatcgtcgac tgccacgatc
     4861 ccggccggcg tgtgggcgat caggacgtcg aggtcgccgt ggctgatccg ccgcatcgag
     4921 ccctcgggca ggtcggccag cgggcctgcg tcgaccggtc cgccgtctgc aacgccgggc
     4981 gccgtcagct cgatcgggaa cgcggccggg atcgggaagg tcgggcggtc gatcgtcatc
     5041 gggccgatcg taccccggaa gacgggaaga ccccggcggg ccgaagcctg ccggggtcga
     5101 tgctgttgcc gcgggttcag tcgaggacgg gttgccctcg ttggccaccc ggctcggggg
     5161 tggagaccgg cacctcggcg ccggtcgtcc tccggagcgg aatctcgtgg aggagcagga
     5221 ccgcgaccac cgcgagcgcg gatgccccga cgccgagcca caagctgtcc gcgatcgcga
     5281 tcgtgaacgc accgtggaag ccgttgagga acgccgcctg gaactgggcg gggatctggc
     5341 tcacgaaatc gagcgggctc gatccgccga ccgacgtcag cgtgccgaga tcgaacccgg
     5401 caggcggcgt tgccggcacg agagccgcag gggcgccggc atcgatgatc tggttcctga
     5461 ggaggtccca gctgaggttg atccggaaga gcgtgaacgc catcgtgatc ccgaccgtcg
     5521 tgccgatctg gcggaacagg gtcaagtcgc tcgtcgcggc cccgagctcg tggaagggaa
     5581 cggagttctg gaccacgatc gtgaagaccg cgaaggtcgg cccgacgccg agaccggcga
     5641 tcaccatcca gatcgccagg gtgaggtcat cggttccggc ccgcagctgg gtcatcaacc
     5701 cgagcccgat gaccaggacg acgatcccga tgatcaaggt tggcttgtac ttgcccgtcc
     5761 ggctgacgac gaggccggac aggatcgagg agaagatcag gccagcgagg aacggcagga
     5821 ggcggtagcc cgactcggtg gcgttcgcgc cctcgacgac ctggaagtag agcggcagga
     5881 agatgatcgc cgctccgaac ccgaacgtcg cgaagaagat cgcgaccatc gacgcgctga
     5941 acgtccggtt ccggaacagg tggagcggaa tgacaggctc ggcggcacgg cgctcgacga
     6001 gcacgaagat cacgaggaag accgcgccga ccgcgctcca gatccagacg tacggatcgc
     6061 cgaagccgcc ggtctcggcg atcgtcaggc cgatgaggat cgggacgagg ccgaggacga
     6121 gggtcgccac gccgaggtag tcgatcgaga tcttcgtcac cttgccgtgg acattcggca
     6181 gcagccggct gatgacgaac agggcgatca gcccgatcgg caggttgacg aagaagatcc
     6241 agtgccagcc gaaatggtcg gtcagatagc cgccgaggaa tggaccgatg atgaaggcga
     6301 gtccgaagac gccgccgaac agaccctggt agcgaccgcg ctcggccggc gtgaagaggt
     6361 cgccgatgac cgcaagggcg atcgggaaga gcgagccggc gccgagaccc tggatgccgc
     6421 ggaagaggat cagctgccac atcgtctggc tgagacccga cagggcggag ccggcgagga
     6481 acagcacgat gcccgcgatc agcaacggct tgcgaccgta aaggtccgac agcttgccat
     6541 agatcgggac gctcaccgtg ctcgtcagca ggtagatcgt gacgacccac gtgtagaggt
     6601 ccgcgccgtt caggtcggtc acgatcttcg ggagcacggg cccgacgatc gtctggtcga
     6661 gcgcgccaag gaacatgccg agcatgacgg ccgcgaggat ttcgaacttg gctcgacggc
     6721 tgagacccag cgcggggtct tcttccgccg ttggagtcag actctcggac tccgtgggga
     6781 atgactccat ccttctcgtc ctcctggaga attgcgggct tgggatgcgg gcggggagcg
     6841 cccggggtca gacgttgcga gcgtgcgggt ggaggtggtc ggtgccgatc tcgtcgatga
     6901 cggccgtccg gaggtcgccg atcgccccga ggatccgggt gagttgctct tgcgagagcc
     6961 gctccaggat cgaccgcaac cggtcctgct tgagggcctc gatctcgtcg cgctggcgcg
     7021 ctccttcggc ggatgcgcga acgatcacga cgcggcggtc atccgggacg cgatcccgct
     7081 cgacgaggcc gcgctcctcc atccggtcga tcaggccggt ggcgttgctg aacgagacgt
     7141 ccagcagatc cgcgagacgg ctcatcggga gatcgccgtg atgctcgagg acccacagaa
     7201 tgtggaggtg cgtcatgctg atcccggcct tgaccatccg gccggtgccg gcgcagcgga
     7261 ggctgccgat catcgcgtgg agctcgtcga cgaccgaatc cacgagctgc ggcggagatg
     7321 cgggggtcga ggcgggcgcg tcaagcgcgt cggtagttcg catgagatga ataatctagc
     7381 gaccgtggat gatcgcgtca agggcgatgg ttcgtcccaa ggcgaggaca cgacgaacga
     7441 ggaggcaacg tgcggttcgc gctgatgatc gagccccagc agggtctgac ctatgacgag
     7501 cagctggcga tcgtccggcg cgccgaatcc gtcggcttcg aatcgttctt ccggtccgac
     7561 cattaccaga gcttccccgg tccgtccggc gagccgacca ccgacgcctg ggcggtgctc
     7621 gcgggtctcg cccgcgagac ctcgacgatc ggtctcggga cgctggtctc gccggtcacg
     7681 ttccggccgg tcggcaacct cgccaaggtc gtcacgacgg tcgacgagat gagcgacggg
     7741 cgcgtcgagt tcggtctcgg tgcgggctgg aatgagctgg agcatcgcga gctcgggctg
     7801 ccgttccccg acatcaagga tcgggcggac ctgatggagg aacagttcgc ggtcctccgc
     7861 gggctgtggg gcgagccgga cggctggtcg ttcgacggcg cgcacgtgca gatcgccgac
     7921 gcgtcgttcc atccgaagcc ggtggcgcgg accggacgac cgaccttcgc caatgggtca
     7981 gcgcgaccgc gactcctcgc cggcggcgag ggttcgccgc gctcgatgcg gatcgccgcc
     8041 cgctacgcgg acgagttcaa cctctcgtcg tcatccccgg agaaggcggc gacgaagttc
     8101 gccgagctgc gggcggcctg cgaggcgatc gggcgggatc cggccacgat gggccgatcg
     8161 gtcatggccg gcgtgctggt cgggcgggac gcggcggagc tcgacgggcg aaaggcggcg
     8221 ctgctggcag ccttcggaaa cgaggccggt ggcgacgact ggttcgacgc ccgcgagccg
     8281 cgctgggtcc tcggcacgcc cgagcaggcg cgggcgatgg tctcgcggtt cgaggcggcc
     8341 ggggccgagc ggctagtgct ccaggacttc ctgccacgcg atctcgcgat ggtcgacctg
     8401 atggccgagg tgctgttctg atcattcgcc acgccacgtg atgttcgcca ggatcgattc
     8461 tgagcgagtg agcacgagcc aatcgacgat acccgtcggc cccgaaggct tcagcccgcg
     8521 gcgtcggccg ccgcgagata gatcgggtcg tcgcgccgga tcaggtcggg gttcgtgccg
     8581 cggacgcggg cgatccgctc ggtcaggagc tggagcgacg tcgcggtccc caacagggcg
     8641 gcgagcggcg ccggcagggc cttggcctcg tcaacgcgga ttcggccggc cggcgtgagg
     8701 tcggcgtcga gctgaccgtg gacgtcccgg gcgacgatcg ccgccgaccg gatcccgacg
     8761 gccttcgccg cggcaagcgc ctggcgcgcc cgcccgaccc gcgccgcccg tgcgtcgcgg
     8821 tcggtcagga ggaggacgag gccggtcgtc ggatccatcg ccggcaggtg accgtggagg
     8881 aacgtctcga ggtcgcgcat cgtcgtcggg agccaggcgg cctcctcgat cttgagcgcg
     8941 agctcacggg cggcgggacg gtccgccccg gaggcaaggg tgatcagggt cgtgcacccg
     9001 gcgaggatcg cggcgatggc ctcggcaccg gcctcgtcgg cggacccgga gatgaggaga
     9061 tcgcggacga ggccgccatc ggtggttcga tccgccagct catcggcgat ggccgccgcg
     9121 gccacgatcg gcgagaggta gccgaccgtg tggcaccagc cgtggtccat ctcgacggtc
     9181 gcgacgacca tgtccggcga cgcgacggcc gcggcagccg gcgattcggc gctgccggtg
     9241 atgagcgcgg tgcgcgcccc ggccgccgcg gacgcctcga gcgcgcggat cgtcgccacg
     9301 gtgccgcctt cgtgcgagat cccgatcacc agcccgccgg tcggcgggtc gagcgcgagc
     9361 tcgaacgcct gcgccacgag gatcgtcgac gggccgctcg gcaggccacc tgcacggagc
     9421 ccttcacgga ggatctcgac gacggcatgg gcggcatgct cgctcgtgcc gcagccggtc
     9481 acgacgatcg acgcgccgct actggccgcc tggccgatcg cccccgccag ccggcctgcg
     9541 gggccctgcg gatcggcgag ccggtcgagg atccgttcgg cgaggaacgg ctcggccgcg
     9601 atcatgtcgg tcatgtggaa cggtggcccg gatcgggcgg acggctgcgg cagctcggcc
     9661 caggggtcgg gagcgggggc aagcggggcg tgtggatcga acgtcgtcat cgatggagta
     9721 tggcgaccgc gatgccgatg caaggcatgg tttcaccgtt cggcggatga tcggggggag
     9781 acggacgtag ctgggagaga cgatgcaggt cgggctgatg gccccgcagg gctggaaggg
     9841 cgggtacgac ggctgggacg cggcgggcgc ctgggcgcgg accgtcgagc ttgcgacgca
     9901 ggccgaggag ctcggcttcg aatccctctg ggtgttcgac cacttccaca cggtcccggt
     9961 gccgaccgat gagatcacgt tcgagtcgtt cagcgtgctc tcggcgctgg cgatgacgac
    10021 gcgtcgcgtc cgcctcgggc acatggtcgt gtgcaccggc ttccggaacc cggcccacac
    10081 ggccaagctg tcgtcgacga tcgacgtcat cagcggcggt cgcttcgagc tcgggatcgg
    10141 ggcgggctgg aagaaggacg agtgggaggc ctacggctac ggcttcccgc cgatcggcga
    10201 gcggatggac gcgttcggcg acgacctcga ggtgatcacc cggatgttcg cgccggggca
    10261 cgcgagctac gagggcgccc acgcccacgt ccgcgatgcg atcaacgtcc cgaaaggcat
    10321 ccagcagcca cggatcccga tcatcgtcgg cggcaacggc gagaagcgga ccgccggcta
    10381 cgcgatcaag ttcgccgacg agctcaacta cgtctttctc gacgcgcccg agatcgcgga
    10441 gcggatgatc tcggtccggg agcgctgcga cgcggagggc cgcgaccccg cgaccctgcg
    10501 cttctcgctg tacgtccgcg acgaggacgt ccgcgacgcg ggccaggcgc gggtcgatct
    10561 ccttgccggc tacgccgcga tcgggctcga tcggatcgtc tgcttcccga cccgctggtc
    10621 cccgacggcg gacgcccagg cggcgttcgc ggccgacgtc cggtcggcgg gcatcgagct
    10681 cgaggtcggg gagccggtca gcgtctgacc gcggacggcc gacgaaccgg cccgatccgg
    10741 tcgatcgggc cgtagcggaa ccagacgcgg ccgacgagag cttcgaccgg gaccggcccg
    10801 tagcggcgcg agtcgatcgg ctgcccggcc ccggccgcga gcagatcggc gtccgatgcg
    10861 tccgagagga gccacgcctc gtcggcggcc aggacgaggt gaccctcgtc gaggtcgacc
    10921 ctcgccccgg gcccggccgc gacacgcttg atcgcgagca ggtcggagtc cggctcgcgg
    10981 aagacgacga tcgcgccgcg gcgcggccag cgggccgtcg tcgggtcgac cagcagccag
    11041 tcgccgggct ggatcgtcgg cgccatcgac gcctcgctga cgacgacccg ccacgggccg
    11101 cgggcgcgct cgcggacggg ctgtaggcgc gcgcggcccc ggatgcgacg aagccccacc
    11161 tcgggccgat ggcgtcgcgg agctccgcct cggacctcgg tgagcaggcc gctggttcgc
    11221 gctcgcagac gcgtgtcggt caggcgggcc ggccggcgac ccgggtcttg ccctcgccac
    11281 cggtcgcctt ccaggctgcg tcgatctcgt cgatcatgtc gaggagcttc tgcgcgtcgg
    11341 cgaggtcggt cgaggccttg accttgctga cctgcttggt cgcgttccag aagagctcgt
    11401 ggaggttcgg gaccttctcg aggtgctccg gcttgaagta gtcgtgccag aggacgtcga
    11461 ggtggtgctt ggcgagatcg gcctgcttct ccttgatccc gatcgcccgg gcggtgaacg
    11521 tcgggtcgct gttggccgcc atcttctcga tgatcctgta aacggactcg gcctcgattc
    11581 gggcctgctc ggggtcgtag acgccgcagg gcaggtcgca gtgggcgtgg accgtcgtcg
    11641 acggggcgag gaacgtgcgg actcggaaca tgggtctcct tctcggggcg ctgaagggcc
    11701 ccggggcgct gaagggctct gggggttcgc ctcatcgtgc ggtccggagc ggtgtatcgg
    11761 agtctcggag cgggccgtcc ggctgtcaaa cgcgtgccgg ttcgggcctg cccgtggggc
    11821 gcgcccggcg cgtcgtgccg gcagcgtccg agatcgttcg ctctgcgacg tcactccatc
    11881 tctgctcgat cggacgacgt cggcgtccga aatcgtcggg cgccggttcg ggtgcattcg
    11941 gctgctcgga gcggtcgagt tcggatcatc gacgcccatt gcgccccgcc gcgttgcctc
    12001 gcccgtcacg gtcgactagc atcccgtttg gcgttcctgc agcgtgcgcc ggcatcgccg
    12061 gcaagcgtcc tgagaaccga gcgcctctgg tgatgcaggt tcgattcctg cttcttctcg
    12121 tcgcgaccgg gtctggcccc gaggcgaccg gatcgccggc catgcaggcc tcgagcctcc
    12181 gccgacgctc cgggacccgc cgcgacgagg caacgtcccg cgccgccatc gaccgatcat
    12241 cccgcgggcc gtccgccggc tcgccagcaa acgcaacgat cgcgaaccgg aggttcgggt
    12301 ggaaatcgtc atcatcgccc tgctcgtcct gctgatcgcc gccgtgctcg ttcgcccggt
    12361 cgtgacggtg gccacgatcc acgactacca gcgcggtctc cgctaccgca acggccgact
    12421 cgtcgggctg ctcgacccgg gaacccacat cgccatccgg ccgttcaccg agatccacgt
    12481 gatggacggt cgccccacct cgatcaccgt gcccggccag gagatcctca cggccgacgg
    12541 ggtggccctc cgggtgagcc tgacggggcg ctacgtcgtg gccgatccgg tggccgcggt
    12601 gaccaacgac cagagctacg tgaccgctct gtacaccgcg ctccatggcg gcctgcgcga
    12661 ggcgctcgcc ggtcggacgg ccgacgaggt catcgccgac cgggcggcga tcgggccggc
    12721 cgtcggcggg gccgtggcga gcgagctcgc ccggatcggg gtggagctgc tcgggatcga
    12781 tgtccgcgac gtgatggtcc cctcggagct gaagcgggcg ttcgccggga tcgtcgcggc
    12841 gcgccgcgag ggcgaggcgg ccgtcgaacg ggcccgcggc gagaccgccg ccctgcgcag
    12901 tctggccaac gcgggacgga tgatcgagga caacccgggg ctcctccagc tgcggatcct
    12961 gcaacagctc ggaggctcgt cggggaacac gatcatgctc ggcctgccgg ggtcggacgg
    13021 gcgctccgag ggcggctcgg caccgcctcc gaccgcggcc gcggcgtcca cccgctcgcg
    13081 gccgtccacc cgctcgcggc cgcccgcccg ccgctcggcc caggactgag cgggccggta
    13141 tcatcgcccg gtgactgaac gcggattgcc cctcgcggac ggtgctccgg cctgtccctt
    13201 cgtggccttc gaggacgatc gcgacgagcg cgcgtccgtc ccggaccacc gccaccgctg
    13261 ctacgccgat cagcgaccgg cgcctcgcgc gctcgcccac caggaggcgt actgcctgtc
    13321 gagcgcgttc cccgtctgtc cgaccttcca ggactgggcc cgccgcgagg ccgcccgatc
    13381 gcgtgacggg gcgccgaccg cgccgagcgc gtcggccggg atgaaccggg cggccaccgg
    13441 ggccgtcgcc gacgccgacg cggccgatgc ggcctcggcc ggggcggccg gggtcggagg
    13501 cgccgcgggc atcgccggtg cggcgggagc gggcgcgagc gggccggcgg cgggccttgc
    13561 cgacacgtcg cgcgacgtcc gacggaccgt ggacgtcgac gattcgcatg ccgacgacct
    13621 cgtccgccgc aacccgccgc gcgactgggc ggccccgccg ccgtggctgg cgtcgaacga
    13681 gccccgtggc cgcggtgacg ccgagccgcc ggcgttcctc ggccaccggg tcaccgagcc
    13741 gggccagggc ctggccggga gcccggccga tcggctggcc ggcgggccgc cgccgcggcg
    13801 ggatgaatcc gacgatgtca actggtcgag cgagagccgg tcggcggccg cgatcggggc
    13861 cgggctcgcg ggcgggggtg cggaacccgg cccgaggagg cgatccgttc cggcctccga
    13921 tggcgagcag gatgacgatg gcgaggcgcg gccagcccgc cggccacgcg cctacgccca
    13981 gcacctcggc gggcccgacg gacccgactg ggagcaaccg cgccgctacg aggcctatcc
    14041 gacgatcaag gcccgcgtcg ggctgccggc ggtaccccgg atcgtcgggg tcgcggccct
    14101 cgtggccgtg ctcgccctcg ccctgttctt cctgccgggc atgctcaaca tcggtggcgg
    14161 aaccgccacg ccgggaggtt cgcccggggc ctcgcttcgg cccgggccca gcgtcagcct
    14221 cgccccgacc gagcagcccg cggcgacgcc gatcatctac acgatcaagc acggcgacgt
    14281 gctctcgaag atcgcagcgg cccacgggat caccctcgag cagctgatgg cggccaaccc
    14341 gacgatcaag aacccgaaca agatcagcga gggccagcag atcacgatcc cgctcccgag
    14401 cgcggccccg ccggacacga tcgacggttc ggccgcgccg tagatcacga tcgcagcaag
    14461 gccggatcgc ctcggatcgc cagggcgaca ccgacgctga gctccgtgaa cgtgccggat
    14521 cacggtcggt cctcacggag gggcctcgag ggccgagcgc ggggctcgtg cccgaacccc
    14581 gagcccgagc cgcgcgaata cctggccaac catgcgccca gggactcttc ggcaaggttc
    14641 gttaggtccg aaccctggac ttgggacctc agttagcctt cggcggctga cggacagcgg
    14701 ggttcgggat cctgccgaag cgaatacctg actcaagagt ctcccggtga ctgtctggcc
    14761 aggtatggac cggacgaagg accggacgaa ggaccggacg aggaccggtc gaacccgggc
    14821 aggcgatggg ccggtcgagg cgctgccgaa ccgggctagc ggggggccag gttgatcgag
    14881 gtgatcttgc cggcctcgac ccggaaggtg gcgtcgaggt ggagcgacgg cgcacccggc
    14941 cggacgacga ggacgtcggc ctcgtaggtg tttccggtgt cgcggacatc gcccgggatc
    15001 atccgcaggc cggcgtcgcc gcggaggaac cagccgccga cgcgctccac accgcggagg
    15061 gtctgggcgc tgtcgccgac gtggatccgc atctccgcgt tctcggccat gagctggacc
    15121 gccgcctcgc gatcgcccgc gttcagcagc tcgaagaacg tatccatcgt gtcgacggca
    15181 cccacggccc gagtgtaccc gtcatgatct ggcgatgccg tccgacctcg ccggccagtg
    15241 ggcgctcgac ccagcggtcg ccttcctcaa tcacggctcg ttcggggcct gcccgcgacc
    15301 cgtcctcgag gcccagcgcg cctggcgcga ccggatggag gcgcagccgg tccagttctt
    15361 cgcccgcgac ctgcccgggc tcctcgccgc cgcccggcgc gacctcggcg cgttcgtcgg
    15421 cgcggaccgg aacgacctcg ccttcgtcgc caacgccacg ggcgcggtca acgcggtcgt
    15481 ccgctcgctc cgcttcgcgc cgggcgacga gctcctgacc gacgaccacg agtacaacgc
    15541 cacgatcaac gtcctgcgcc acgtcgcgga gcgcgacggc gcccgggtcg tcgtcgccac
    15601 gatcccgttc ccggtcgcct cggaggatgc cgtcgccgac gcgatcctcg gcgccgccac
    15661 cgaccggacg aggctcgccc tcgtcagcca cgtcacgagc ccgaccgcgc tcgttttccc
    15721 gatcgagcgc atcgtcgcgg gcctcgccga gcgcggcgtg gagacgctgg tcgacggggc
    15781 ccatgcgccg ggcatgctgg cgctcgacct cgagcggatc ggcgccgcct ggtacaccgg
    15841 caacctccac aagtgggtct gcgcgccgaa gggcgcggcc ttcctccacg cccggcgcga
    15901 ccgccagccg ggcatccgcc cgaacacgat ctcccacggc gcgaacgaga tcatcggtgc
    15961 cgatcgggtc gacggacgga cgcgctaccg ggcggagttc gactggcagg ggaccctgga
    16021 cccgaccgcg tggctggcgg tctccgaggc attgcggttc gtcggcgggc tggtcgatgg
    16081 cggctggccc gaggtgatgg cgcgcaaccg ggcgatgaca ctccggacgc gagtggcgat
    16141 gaccgccgtg ctcgggctcg acgacgcggt gcccgccccg gagtccatgc tcggctcgct
    16201 cgtcgcgctg ccgctgccgg ctttcggtcc gctggccgcc cgtggttcgg ggtcgtcgcc
    16261 gctcgacacc gacccgctcc agcagcgcct catcgaggag ttccaggtcg aggtcccgat
    16321 ctatccgtgg cccgttccgg ccgccgaatc gccgcaggcg tcgcgtcggc tgatccgcgt
    16381 gtcgagcgcg ctccacaacg ggcccgacga cgtcgagtgc ctggtcgcgg cactcggcgc
    16441 gat
//
LOCUS       DATAIW010000081         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_239952, whole genome shotgun sequence.
ACCESSION   DATAIW010000081 DATAIW010000000
VERSION     DATAIW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239952"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1046
                     /locus_tag="VHM48_02820"
     CDS             159..1046
                     /locus_tag="VHM48_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX2754365.1"
                     /translation="MRAAADVDRDRQPLSRRVVVFGAASWNMLIRVERFPDPGPATVF
                     PPGWHETVGSSGAGKALNLARLGVPVTLHCLLGDDQPGARIRTMLEAAGVTVDAVTDP
                     TGTARHVNLMDPAGRRLSFLLHTGDPAIRYNPDRVAGLVAAADHVVVEIVDQARVVLP
                     IARRLGKPTWTDLHATDGERAWERDFWSADRVLFSGEHLADPRPFMERLRRDGRLLVV
                     CTRAEHGTVALTADGRWIEIPAEPVATVVDTNGAGDAFAAGTIAGVMRGDPVDDSLRL
                     GAHVAALAVQSADLAPPSV"
     gene            1075..1359
                     /locus_tag="VHM48_02825"
     CDS             1075..1359
                     /locus_tag="VHM48_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754366.1"
                     /translation="MPVWLVAILVVAYLAAPVVELRLWRAGRLSDRAVAVLLLTRFPV
                     LLGVMWLAAGVHLDTLVIIVPVVIVIWKIGYGWLLSDLRDRGGELRSARS"
     gene            complement(1368..>1906)
                     /locus_tag="VHM48_02830"
     CDS             complement(1368..>1906)
                     /locus_tag="VHM48_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754367.1"
                     /translation="GVFAAIPLVADRQKLILKRLQATPLRRWQLVGSNVLMRLLIAIF
                     QTAIIVGVGSALYHVQIAGSWLLIGALILLGSLAFIALGYVIASFARTEESANGMTSV
                     VQFPLMFLSGTFFPIDQMPDALKTVARALPLTYLGDGLRQVMVEGTPFAPLWVCFLFL
                     AVWLAVCFGIAARFFKWQ"
BASE COUNT          275 a          700 c          649 g          282 t
ORIGIN      
        1 cgcccggcgc ctgctcggct accccgacga ccaccactgc gagtacctga tctcgttcgg
       61 gtatcccgcc gaccccgacg tcctcacccg gccgaacaag gcggggggac ggcaggccct
      121 cgccgacatc atccacgacg agcgctggta gcggacgggt gcgggccgcc gcggacgtcg
      181 accgcgaccg tcagccgctg agccggcgcg tcgtcgtctt cggcgcggcc tcctggaaca
      241 tgctgatccg ggtcgagcgg ttccccgacc cgggaccggc gaccgtgttt cctcctggct
      301 ggcacgagac cgtcgggtcg tcgggggccg gcaaggcgct caacctggcg cggctcggcg
      361 tcccggtcac cctccactgc ctcctcgggg acgatcagcc cggtgctcgc atccggacga
      421 tgctcgaggc cgccggtgtc accgtcgatg ccgtcaccga tccgaccggc acggcccgcc
      481 acgtcaacct gatggatccg gccggccgcc gcctctcgtt cctgctccac accggcgatc
      541 ccgcgatccg ctacaacccg gatcgtgtcg ccggcctggt cgccgccgcc gatcatgtcg
      601 tcgtcgagat cgtcgaccag gcccgggtcg tcctcccgat cgcgcggcgc ctcggcaagc
      661 cgacctggac cgacctccac gcgaccgacg gcgagcgcgc ctgggaacgc gacttctggt
      721 cggccgaccg ggtgttgttc agcggcgagc acctcgccga tccgcgcccg ttcatggaac
      781 ggctgcgacg cgacggccgg ctgctcgtcg tctgtacccg ggcggagcac gggacggtcg
      841 ccctgacggc cgacggccgg tggatcgaga tcccggccga gccggtcgcg accgtcgtcg
      901 acaccaacgg cgccggcgac gcgttcgccg cgggcacgat cgcgggggtg atgcggggcg
      961 atccggtcga cgactccctg cggctgggcg cccacgtcgc cgcactggcc gtccagtcgg
     1021 ccgacctcgc gccgccgtcc gtctgaccga ttcgccgggg gctggaatcc gaccatgccc
     1081 gtctggctgg ttgcgatcct ggtcgttgcc tacctcgctg ccccggtcgt cgagctccgg
     1141 ctgtggcgcg ccgggcgtct gtcggatcga gccgtcgccg tcctgctgct cacccgattc
     1201 ccggtcctgc tcggcgtcat gtggctggcc gcgggcgtcc acctcgatac gctcgtgatc
     1261 atcgtcccag tcgtgatcgt catctggaag atcgggtacg gctggctgct ctcggacctt
     1321 cgcgatcgag gcggggagct acgctcggcc cggagctgag atcgggctca ctgccacttg
     1381 aagaaccgcg cggcgatccc gaagcagacc gccagccaga cggcaaggaa caggaagcac
     1441 acccagagcg gcgcgaacgg cgtcccctcc accatgacct ggcgcagccc gtcgccgaga
     1501 taggtcagcg gcagggcgcg ggccaccgtc ttgagggcat cgggcatctg gtcgatcggg
     1561 aagaacgtcc ccgacaggaa catcagcggg aactgtacga cgctcgtcat cccgttggcg
     1621 ctctcctcgg tcctggcgaa cgatgcgatc acgtagccga gggcgatgaa cgcgagcgat
     1681 ccgagcagga tcagcgcccc gatgagcagc cacgacccgg cgatctggac gtgatacagc
     1741 gccgatccga ccccgacgat gatcgccgtc tggaagatcg cgatcaggag ccgcatcagg
     1801 acgttgctcc cgacgagctg ccagcgccgg agcggcgtcg cctggaggcg cttgaggatc
     1861 agcttctggc gatcggcgac gagcgggatg gcggcgaaca ccccga
//
LOCUS       DATAIW010000082         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_152780, whole genome shotgun sequence.
ACCESSION   DATAIW010000082 DATAIW010000000
VERSION     DATAIW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152780"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1683)
                     /locus_tag="VHM48_02835"
     CDS             complement(<1..>1683)
                     /locus_tag="VHM48_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX2754368.1"
                     /translation="PEPVEFGQVDGTIKRLYVPDDGSALIVVTTDDQVRTLDPVSGEV
                     LGAVQLEAVADVGPGGTASTLVSAPGAVGDPHALASALAKVIGGAAATYEARLAGTGE
                     QLIIAGITGSTQQAAIQKAIDDGRLAGLSITSLPQVAVADANGLELINPTDGSKTATV
                     DLGGAAHGLALATVDSARLYVSVDPDPAVKEKGRIAIVAVGGDQAKNGPAVVDTMAMP
                     GPVTKVAYDDATEMVHVLGRTPDGTASTIYVIQPHADPGPQPAVYADARLPFDPSAWA
                     VDVNRPYPTDDRQQILVFDGEGATASVDVREHEFAWRLPGVLAGTAMAGFLFVLARIL
                     FRRRTVGLFVAVMCVADGMLFVQSRIGMNDAYVGLGIVAAYTLFAALWTGVWRHRGAF
                     WIAMPLIGAFLGLALASKWVALYAIVGMGFLVLVRSALGRLLAIAALVVMTAVLGYLA
                     INVPQGTGFGNLPFVAIMVALTVVAVVANVLHPLRWSLDEIRFAIGGPVALGGFVALG
                     AIGLGKATATLKLGPATVNPLEIAAALALVGVVAWLAFAGASRLGFGPFAPAP"
BASE COUNT          260 a          610 c          608 g          205 t
ORIGIN      
        1 ggagccgggg cgaacgggcc gaacccgagc cgggacgccc cggcgaacgc cagccacgcc
       61 accaccccga cgagggccag cgcggccgcg atctcgagcg ggttgaccgt cgccggcccg
      121 agcttgagcg tggccgtcgc cttcccgagc ccgatcgcgc cgagcgcgac gaacccgccg
      181 agcgccaccg ggccgccgat cgcgaaccgg atctcgtcga gcgaccagcg cagcgggtgg
      241 aggacgttgg cgacgaccgc gacgacggtc aacgcgacca tgatcgcgac gaacggcagg
      301 ttgccgaatc cggtgccctg cggaacgttg atcgcgaggt agcccagcac cgcggtcatg
      361 accaccagcg cggcgatcgc gaggagccgc ccgagcgcgc tccggaccag gacgaggaag
      421 cccatcccga cgatggcgta gagcgcgacc catttcgagg cgagggcgag cccgaggaac
      481 gccccgatca ggggcatcgc gatccagaac gcgccacggt gacgccagac ccccgtccag
      541 agcgcggcga agagcgtgta ggcagcgacg atccccagcc cgacgtaggc gtcgttcatc
      601 ccgatccggg actggacgaa cagcatcccg tcggccacgc acatcacggc cacgaacagc
      661 ccgaccgtcc ggcgccggaa caggatccgg gcgaggacga acaggaagcc ggccatcgcc
      721 gttccggcga gcaccccggg cagccgccac gcgaactcgt gctcccggac gtcgacgctc
      781 gcggtcgccc cttccccgtc gaagacgagg atctgctggc ggtcgtcggt cggatacggc
      841 cggttgacgt cgaccgccca ggccgacgga tcgaacggca ggcgggcgtc ggcgtagacc
      901 gcgggttggg ggcctggatc cgcgtggggc tggatgacgt agatcgtcga cgccgtcccg
      961 tcgggggtcc ggccgaggac gtggaccatc tcggtcgcgt cgtcatacgc gaccttcgtg
     1021 acgggtccgg gcatcgccat cgtgtccacg accgccggcc cgttcttcgc ctggtcgccg
     1081 ccgacggcga cgatcgcgat ccggcccttc tccttgaccg ccggatcggg atcaacagag
     1141 acgtacagcc gggcgctgtc gaccgtcgcc agggcgagcc cgtgagccgc gccgccgaga
     1201 tcgaccgtcg cggtcttcga gccgtcggtc gggttgatca gctcgagccc gttggcgtcg
     1261 gcgacggcca cctggggcag cgaggtgatc gagaggcccg ccagccggcc gtcgtcgatc
     1321 gccttctgga tcgccgcctg ctgggtgctg ccggtgatcc cggcgatgat cagctgctcg
     1381 ccggttccgg ccaggcgcgc ctcgtaggtc gccgcggcgc caccgatgac cttcgccagg
     1441 gccgacgcca gcgcgtgcgg atcgccgacg gcgcccggag cgctgaccag ggtcgacgcc
     1501 gtcccgcccg gtccgacgtc ggccacggcc tcgagctgga cggcgccgag gacctcgccc
     1561 gaaaccgggt cgagggtccg gacctggtcg tcggtcgtga cgacgatgag ggccgagccg
     1621 tcgtcgggga cgtacaggcg cttgatcgtg ccgtcgacct ggccgaactc gaccggctcc
     1681 ggg
//
LOCUS       DATAIW010000083         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_3513, whole genome shotgun sequence.
ACCESSION   DATAIW010000083 DATAIW010000000
VERSION     DATAIW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1863)
                     /gene="uvrC"
                     /locus_tag="VHM48_02840"
     CDS             complement(13..1863)
                     /gene="uvrC"
                     /locus_tag="VHM48_02840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX2754369.1"
                     /translation="MTPDLQATLKKLPDRPGVYLMRDARGDVVYVGKAQSLRSRVRSY
                     WQKATPVGEVHRIRSVIDRVQDVEYTLTDSISEALLLEANLIKRYRPRFNVRLKDDKS
                     YPYIKITMADDFPRVERTRKLIEDGSRYFGPYASASSVDESMNLVRRLFPFRTCTIDI
                     EDGKRALARPCLLFHIKRCQGPCIEAVSKADYRGQIDQIELFLEGRQESVVQALSAEM
                     KTASEKTDYERAATIRDKIRAIERTMESQKMAAFKRTQLDMVGLARQDNQAAIQLFAI
                     RGGKMIGRDVFLLDAAREASDEEVLSSFLLQYYARATSIPPQVLVPCAPPDAADLEAF
                     LTERRGGPSRLAVPQRGEKRELMALATRNAGETLAREQARWMADEGKTLGALEELAEA
                     LGLAGPPLRIECYDISNVQGSDSVGSMVVFEEGKPRTGEYRRFKIKTVVGANDFASHQ
                     EVLRRRFRRAKDGDEGAEEALRWAMPDLVIIDGGKGQVSAVKEVLDELGLHDLPLAGL
                     AKEREELFRPGIRVPVVLPPTSSALYLVQRLRDEAHRFAITYHRDLRSKRQTHSAFDD
                     LPGVGPKRRRALLRVFGSAKRVREAPVEQIAAVPGIGAALAARIKETLEA"
     gene            complement(1944..3290)
                     /locus_tag="VHM48_02845"
     CDS             complement(1944..3290)
                     /locus_tag="VHM48_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX2754370.1"
                     /translation="MQRRSIRRALVAASTLVILAAVGACADTVPADGDAVTPGNQSLV
                     VLPDATPGQVVTWPINFRLTCDGLNHAAPGATIQLDLDSATVPLGGDASATSTSIGPV
                     PADWALDGCASPAPTIAANGPSVVTLTMPPTAGGYEFTIFWSRLGATGLTGSSVITFQ
                     VNVVGNTPPTLHLPSGILAEATSPAGAAVKWSATATDAEDATPPTPTCSPASGSTFPL
                     GITTVHCSVTDGGGLKDSGSFLVTVEDTTVPKLVGMPADQNLTTGDPAGTTVTFTPPT
                     ATDTADPSPTVGCVPASGSHVAVGTTTVTCTAQDATGNHVSATFAVNVTYVPSIVWSA
                     TWGEPVGSSGATFVANAGRTVPVKVEMFADGVEQTHGVAVLTLATCAGTGAGTVGLSW
                     DGGRWAGHLDTSMLGGPGCYVATASLDGNVAGSFRIDLRGADPAVTTGGPKGRAKG"
     gene            complement(3533..>4238)
                     /locus_tag="VHM48_02850"
     CDS             complement(3533..>4238)
                     /locus_tag="VHM48_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754371.1"
                     /translation="TATVVDTDTAGTKLPPTGTVTFTRDGGLPVTCPTLTSTGTDRSA
                     CSVTWTSATPQVWVVVAKYNGSDVHVTSTSEAQIVVFYDPSGGFVTGGGYIKHEDPIG
                     AANPNTPGVAGKDNFGFNAKYLKNATVPTGETEFQCKVCNLNFHSTSYDWLVITRLST
                     TTVKAQYRGLGTINGAAGYGFIVTVIDGGQTDTARIRIWNTLTGATVYDNEYPKPDSA
                     NPTTITAGGNIVVHDK"
BASE COUNT          581 a         1397 c         1555 g          705 t
ORIGIN      
        1 gtcgggccag gctcaggctt ccagcgtctc cttgatccgg gcagccagcg cggcgccgat
       61 ccccggaacc gcggcgatct gctcgaccgg cgcctcgcgc acccgctttg ccgacccgaa
      121 cacgcgcagc agggcgcgcc gccgcttcgg gcccacgccc ggcaggtcgt cgaacgccga
      181 gtgcgtctgg cgcttcgagc ggaggtcgcg gtgataggtg atcgcgaagc ggtgcgcctc
      241 gtcgcgcagc cgctggacga gatagagggc cgacgaggtc ggcggcagca cgaccggcac
      301 ccggatcccg ggacggaaca gctcctcgcg ctccttggcc agccccgcca gcggcaggtc
      361 gtgcaggccc agctcgtcga ggacctcctt gaccgcgctg acctggccct tgccgccgtc
      421 gatgatcacg aggtccggca tcgcccagcg gagcgcctcc tcggctccct cgtcgccgtc
      481 cttggctcgg cggaaacggc gacgcaacac ctcctgatgc gacgcgaagt cgttggcacc
      541 gaccacggtc ttgatcttga aacggcggta ctcgccggtc cgcggcttgc cctcctcgaa
      601 cacgaccatg ctcccgaccg agtccgagcc ctggacgttg gagatgtcgt agcactcgat
      661 ccggagcggt ggcccggcca gcccgagcgc ctcggccagc tcctcgaggg ccccgagcgt
      721 cttgccctcg tcggccatcc agcgggcctg ctcgcgggcg agcgtctcgc cggcgttgcg
      781 ggtggcgagg gccatcagct cgcgtttctc gccgcgctgc gggaccgcca gccgcgacgg
      841 gccgccgcgc cgctccgtca gaaaggcctc gaggtctgcc gcatcgggcg gcgcgcacgg
      901 aacgaggacc tgcggcggga tcgacgtcgc gcgcgcgtag tactgcaaca ggaagctcga
      961 gaggacctcc tcgtccgacg cctcgcgggc ggcgtcgagg aggaacacgt cgcgcccgat
     1021 catcttgccg ccgcggatcg cgaagagctg gatcgccgcc tggttgtcct ggcgggcaag
     1081 gccgaccatg tcgagctggg tccgcttgaa cgcggccatc ttctggctct ccatcgtccg
     1141 ttcgatcgcc cggatcttgt cgcggatggt ggccgcccgt tcgtagtcgg tcttctcgga
     1201 ggcggtcttc atctcggccg acagcgcctg gacgaccgac tcctggcggc cctcgaggaa
     1261 gagctcgatc tggtcgatct ggccgcggta gtcggccttc gaaacggcct cgatgcaggg
     1321 gccctgacag cgcttgatat gaaagagcag gcatggccgg gcaagggcgc gcttgccgtc
     1381 ctcgatgtcg atcgtgcagg tccggaacgg gaagagccgg cggaccaggt tcatcgactc
     1441 gtcgacgctg ctcgccgacg cgtacggccc gaagtagcgg ctgccgtcct cgatgagctt
     1501 gcgggtccgc tcgacgcggg ggaagtcgtc ggccatcgtg atcttgatgt aggggtagct
     1561 cttgtcgtcc ttgagccgga cgttgaaccg cggccggtag cgcttgatca ggttggcctc
     1621 gagcaggagt gcctcgctga tcgagtcggt cagggtgtac tcgacgtcct ggacgcggtc
     1681 gatgaccgag cggatccggt gaacctcgcc gaccggcgtc gccttctgcc agtagctccg
     1741 gacacggctc cgcaggctct gcgccttgcc gacgtagacg acgtcgcccc gggcgtcgcg
     1801 catgaggtag acgccgggtc ggtcgggcag cttcttgagg gtcgcctgga ggtcgggggt
     1861 cacgccccga tgctaggaca cccggcgcga ggatgcgccg ggtgtcatgt cgttggtcgt
     1921 gggtcgcgcc gtggcgcgtc tcgtcagccc ttggcgcgac ccttcggccc gccggtcgta
     1981 acggccgggt cggcgccacg gaggtcgatc cggaaggatc ctgcgacgtt gccgtcgagg
     2041 ctggccgtgg cgacgtagca gcccggcccg ccgagcatgc tcgtgtcgag gtggcccgcc
     2101 caacgcccgc catcccacga caggccgacg gtcccggctc cggtccccgc gcaggtcgcg
     2161 agcgtcagca cggccacgcc atgcgtctgc tcgacgccgt cggcgaacat ctcgaccttg
     2221 acgggaacgg tccggccggc gttcgcgacg aacgtggccc cgctcgatcc gaccggctcg
     2281 ccccaggtgg ccgaccacac gatcgacggg acgtaggtca cgttgacggc gaacgtcgct
     2341 gacacgtggt tgccggtcgc gtcctgcgcg gtgcaggtga cggtcgtcgt gccgaccgcg
     2401 acatggctgc cgctcgcggg cacgcaaccg acggtcggcg atggatcggc cgtgtcggtg
     2461 gccgtgggtg gggtgaatgt cacggtcgtg ccggccgggt cgccggtcgt caggttctgg
     2521 tcggccggca tcccgaccag cttcggaacc gtggtgtcct cgacggtcac gaggaacgag
     2581 ccgctgtcct tcaggccgcc gccgtcggtg acggagcagt ggacggtggt gatcccgagc
     2641 gggaaggtcg agcccgaggc cggcgaacac gtcggggttg gcggggtggc gtcctcggca
     2701 tcggtcgcgg tcgccgacca tttgacggcg gcgccggcgg gcgaggtggc ctcggccagg
     2761 atgccggacg gcaggtggag cgtcggcggg gtgttgccga cgacgttcac ctggaaggtg
     2821 atcacgctgc tgccggtcag gccggtcgcg ccgaggcgtg accagaagat ggtgaactcg
     2881 tagccgccgg cggtcggggg catcgtcagc gtgacgacgc tgggcccgtt ggccgcgatc
     2941 gtcggtgcgg ggctggcgca accgtccagg gcccaatcag ccgggaccgg cccgatcgac
     3001 gtcgaggtcg cgcttgcgtc gccaccgagc gggacggtcg cgctgtcaag gtcgagctgg
     3061 atcgtcgcgc cgggggcggc atggttcagg ccgtcgcagg taagccggaa gttgatcggc
     3121 caggtcacga cctggccggg cgtcgcatcc ggcaggacga ccagggattg gttgccggga
     3181 gtcacggcgt cgccgtccgc cggtacggtg tccgcacagg ccccgaccgc agccaggatc
     3241 accagggtcg aggcggccac cagggctcgc cggatcgaac gtcgttgcat gtgccgaacc
     3301 tcgcctcacg cgcaggaagg tgctgactcg acgcatggtc cggggttgtc gtgggaccgt
     3361 cgatgggccg atcggggtca aatgtgcggc cgtgcgcccc caaggagtac cggtgccgcg
     3421 aatcaccggc cggcgcggac gcggctcaac agggcttctg cggccgggaa tggtcgaggg
     3481 ccggtccccc tgaaggaccg gccctcgacg cgcgctcgga agcgggtgat tgctacttgt
     3541 cgtggacgac gatgttgccg ccggccgtga tggtggtcgg gttggcgctg tcaggcttcg
     3601 gatactcgtt gtcgtagacg gtcgccccgg tgagggtatt ccagatgcgg atccttgcgg
     3661 tatctgtctg gccgccgtcg atcacggtca cgatgaagcc gtagccggcc gcgccgttga
     3721 tcgtccccag gcccctgtac tgggccttga ccgtggtggt cgagagcctg gtgatcacga
     3781 gccagtcgta cgaggtgcta tggaagttga ggttgcagac cttgcactgg aactcggtct
     3841 ccccggtcgg gacggtcgcg ttcttcaggt acttggcgtt gaagccgaag ttgtccttcc
     3901 cggcgacacc gggcgtgttc gggttggccg cgccgattgg gtcctcgtgc ttgatgtagc
     3961 caccgccggt gacgaagcca ccgctcgggt cgtagaagac aacaatctgg gcttccgagg
     4021 tgctcgtgac atgcacgtcg ctgccgttgt acttcgcgac gaccacccag acctgcggtg
     4081 tggcggacgt ccaggtgacg ctgcatgcgg accggtccgt tccagtgctt gtgagagtcg
     4141 ggcatgtgac gggcaggccg ccgtcacggg tgaacgtgac cgtgcctgtc ggcgggagct
     4201 tcgtgccggc ggtgtcggtg tcgacgacgg tcgccgtg
//
LOCUS       DATAIW010000084         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_3552, whole genome shotgun sequence.
ACCESSION   DATAIW010000084 DATAIW010000000
VERSION     DATAIW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VHM48_02855"
     CDS             complement(<1..377)
                     /locus_tag="VHM48_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754372.1"
                     /translation="MNTMSLGVVAQATDHEVAEGSTGSRWSGVSVAAAVALAAAFVGL
                     AFRVPFLALGIPAVMIAGWWLGPKVRSTEDIVGVGLAMATTTVALADALVVSGSAISA
                     VVGAGRFGGPSLLETLAGSVFMWG"
     gene            415..1182
                     /locus_tag="VHM48_02860"
     CDS             415..1182
                     /locus_tag="VHM48_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754373.1"
                     /translation="MTTELATVKSHVSDGGDGAPRDPIGPAERLRLQFAEAWGEMSAS
                     WGVAPAIGRVHAYLMVRNEPLTEREIREALGLSHRAASLALTDAEIWGIVERVSEPRR
                     VGRRGPAGTAYVAVGDHWQWFTRVIAERKVREGDPIVEVIARTAGQAGALAAADPADT
                     ELAALRDWLITFNAFVRLFDRSVGLIPKLAPRELERGMRLLGEVSDETVLRLVHLLGS
                     LPDDDVLELIEALSRLSPTAARRATKLMSGVIRTVAR"
     gene            1526..>2379
                     /gene="pdxS"
                     /locus_tag="VHM48_02865"
     CDS             1526..>2379
                     /gene="pdxS"
                     /locus_tag="VHM48_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2754374.1"
                     /translation="MTVETSTWATKKGLAQMLKGGVIMDVVTPDHAKIAEDAGAVAVM
                     ALERVPADIRAAGGVARMSDPSMIEGIMAAVTIPVMAKARIGHFVEAQVLEALGVDYI
                     DESEVLTPADETNHIDKHAFKVPFVCGCRNLGEALRRINEGAAMMRTKGEAGTGNIVE
                     AVRQLRDVLGAIRRLQTMREDELFVAAKELQAPYELVKQVATDGRLPVVNFVAGGIST
                     PADAALVMQLGAEGVFVGSGIFKSEDPARMANAIVQATTHFDNPKIIAEVSKGLGAPM
                     RGIAMEAIP"
BASE COUNT          380 a          842 c          817 g          340 t
ORIGIN      
        1 ccccacatga agacggatcc ggcgagcgtc tccaggagcg aagggccgcc gaaccggccg
       61 gcgccgacga ccgccgagat cgccgatccc gacacgacga gggcatcggc cagcgcgacg
      121 gtcgtcgtcg ccatcgcgag gcccactccg acgatgtctt cggtcgaccg gaccttcggg
      181 cccagccacc agccggcgat catgacggcc gggattccga gcgccaggaa cgggacccgg
      241 aacgcgagcc cgacgaacgc cgcggccagg gcgaccgctg cagcgacact cactccggac
      301 caacggctcc ctgtcgagcc ctcggccacc tcatggtcgg tcgcctgcgc gacgaccccg
      361 agcgacatcg tgttcatcgt cggatccttc cgtgctactc ttatatagca agttatgacg
      421 actgaactcg ctactgtcaa gtcgcacgtt tctgatggcg gtgacggcgc gccccgcgac
      481 ccgatcgggc cggccgagcg ccttcggctc cagttcgccg aggcgtgggg cgagatgagc
      541 gcgtcgtggg gcgtcgcgcc tgccatcggg cgagtccacg cctacctcat ggtccgcaac
      601 gagccgctta ccgagcgcga gatccgcgag gcgctcggtc tgtcgcatcg agcggcgagc
      661 ctcgccctga cggacgccga gatctggggg atcgtcgagc gtgtgtcgga accgcgaaga
      721 gtcggtcgac gcggtccggc cgggaccgcc tatgtggcgg tcggcgacca ctggcagtgg
      781 ttcaccaggg tgatcgccga gcgcaaggtc cgcgagggcg acccgatcgt cgaggtcatc
      841 gccaggaccg ccggccaggc cggtgcgctc gccgcggccg acccggccga cacggagctg
      901 gcggcactgc gcgactggct catcaccttc aacgcgttcg tccgtctgtt cgaccgctcg
      961 gtggggctga tcccgaagct cgccccgcgc gagctcgagc gcgggatgcg gctcctcggc
     1021 gaggtgtcgg acgagacggt cctgcgcctc gtccacctgc ttggctcgct gcccgacgac
     1081 gacgtcctcg agctgatcga ggcgctcagc cggctgtcac cgacggccgc ccggcgggcc
     1141 acgaagctga tgtcgggggt catccggacg gtcgcccgat aggaacgtcc accgcctcgg
     1201 gtgtcttccc atacgccagc cccgatcgcg taaggagcgg gttcgcgcct cgtgccgtcg
     1261 gttaccaggc gtttgtcagc cgccaacggc taactggcgc cgtggtcgcg ctcgatccgg
     1321 gccgagacct cgccgaacgt ttaccaggct gtcgtatggg aatctccgcg cgccgggtcc
     1381 aggaaccggt gccgatcgtg ggtatcgccg ggcgccgtgc gataatcatg agcgcgcgag
     1441 gcggcccgac cgactcgaac gggcgacgac cccgcggcca gcccggccgg cagacccgcg
     1501 cagcgacacc ggagccacac tgcacatgac cgtcgagacg tccacctggg cgacgaagaa
     1561 ggggctcgcc cagatgctga agggcggcgt catcatggac gtcgtcaccc ctgaccacgc
     1621 gaagatcgcc gaggatgccg gcgccgttgc cgtcatggcc ctcgagcgcg tcccggccga
     1681 catccgcgcc gccggcggcg tcgcccggat gagcgacccg tcgatgatcg aagggatcat
     1741 ggccgcggtc acgatcccgg tcatggccaa ggcccggatc ggccatttcg tcgaggctca
     1801 ggttcttgag gccctcggcg tcgactacat cgacgagtcc gaggtcctga ccccggccga
     1861 cgagacgaac cacatcgaca agcatgcctt caaggttccg ttcgtgtgcg gctgccggaa
     1921 cctcggcgag gcgctccgcc ggatcaacga gggcgcggcg atgatgcgga ccaagggcga
     1981 ggccggcacc ggcaacatcg tcgaggcggt ccgccagctg cgcgacgttc tcggcgcgat
     2041 ccgtcgcctc cagaccatgc gcgaggacga gctgttcgtc gcggccaaag agctccaggc
     2101 gccgtacgag ctggtcaagc aggtcgcgac cgacggcagg ctgccggtcg tgaacttcgt
     2161 cgccggcggg atctccaccc ccgccgacgc cgcgctcgtc atgcagctcg gcgccgaggg
     2221 tgtcttcgtc ggctcgggca tcttcaagag cgaggatccg gcccggatgg cgaacgcgat
     2281 cgtccaggcg acgacccact tcgacaaccc gaagatcatc gccgaggtgt cgaagggtct
     2341 gggcgccccg atgcggggca tcgcgatgga agcgatccc
//
LOCUS       DATAIW010000085         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_152886, whole genome shotgun sequence.
ACCESSION   DATAIW010000085 DATAIW010000000
VERSION     DATAIW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152886"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VHM48_02870"
     CDS             <1..471
                     /locus_tag="VHM48_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2754375.1"
                     /translation="CAMLLVRRPADFDVIVTENLFGDILSDEAAVLAGSLGMLPSASL
                     GERRTAHGAFGLYEPIHGSAPDIAGRDLANPIGTILSAAMLLRLSLGREDAAAAIEAA
                     VSQTLDHGFRTADLVPPSGDETGLRRVGTVGMTAAIVERIAIAEAARATAAART"
     gene            509..2194
                     /gene="cimA"
                     /locus_tag="VHM48_02875"
     CDS             509..2194
                     /gene="cimA"
                     /locus_tag="VHM48_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX2754376.1"
                     /translation="MTTPTDLPVTLYDTTLRDGTQGENITLSLADKLRIARMLDEYGM
                     PYIEGGWPGSNPKDIEFFKAARSMTWQTAKLAAFGSTRHRSNTAENDPNLRELVAAET
                     PVVTIFGKSWLLHVVEVLGATPQENLDMVADSVRFIVERGRELVYDAEHFFDGYRDDR
                     DYALATLRAARQAGARTLVLCDTNGGTLTDELVRIVTDCRSSLEGDPGADGTPITWGI
                     HTHNDAELAVANSMAAVAVGVRHVQATINGYGERAGNANMVSILANLALKTAHELVPA
                     GGGTLTGLTELSRSVAEIANINPNDWQPYVGRSAFAHKGGVHGAAVAKVERSYQHIEP
                     TAVGNVGRLVVSELGGRANTRIRAEQLGHQLEGVIDPKALSELVKQLESEGLAFEGAE
                     ASFELLIRRHQADYVAPFRIVDYTCLVEQRSGQELLSEATVKVQVDGEILHTAADGNG
                     PVNALDAALRKALRAFYPQLDDVHLYDYKVRILDGDSATAARTRVIIDSSDGALEWST
                     MGSDTNIIAASAKALADSLEYAIWKSGAELVRRDERHFTTTPASPAATSRTGR"
     gene            2197..2763
                     /locus_tag="VHM48_02880"
     CDS             2197..2763
                     /locus_tag="VHM48_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX2754377.1"
                     /translation="MAADTTPAATAPTGDAADTLHLTRWTVTSGSNANSRGAVVISAG
                     DHQWQASAEGNGAVDALFRAVDKALAGVLTGQPRLLSYDVHAVAEGPDAAGKVTVAIA
                     PPADASGPRASGRYVGDITSTNIIAASIEAYVDAVNALLAEEHWQGATETAGNRRRAR
                     VSEPAAKAQRAELDEEAGRIDTTDWFNH"
     gene            3087..3953
                     /gene="pheA"
                     /locus_tag="VHM48_02885"
     CDS             3087..3953
                     /gene="pheA"
                     /locus_tag="VHM48_02885"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX2754378.1"
                     /translation="MKSETQVVYQGEPGAFSEEAVLSFFGESRERTAVPTWRAVFEAV
                     ADGSAGGGVIAIESSLAGTIRETYDLLAEFYDRDVRIVGEVTVPVRLALLALPGQSLD
                     WIERVYSHGQALVQADAFLRSREWQVMTTYNTAGAARMIAERHERHAAAVASPRVAAL
                     YGLEILAADIQSGDENRTRFAVLSRRGPEGAHGAAPVDPVGPRLTTLVFAVRNLPGSL
                     HRCLGAFAARGVNLSSLESRPARRARWEYVFWVDVDAAADEPACAAALDDLRAETEMV
                     KVLGSYPRARED"
     gene            complement(3988..4626)
                     /locus_tag="VHM48_02890"
     CDS             complement(3988..4626)
                     /locus_tag="VHM48_02890"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX2754379.1"
                     /translation="MVVTAGPVIFEHPVDRDAELRGGAGIVRFLDLPATRAVMIDGDG
                     APGPDAFVPRMPGLYTTAYKVRFGLKARGVVTKVGPLEGLWWTSDGSTDLDAVFGGAG
                     ERGTWRWTLLIALPDEATDDELERAVGAGRAKLGEAFAPSLRIEALAEGRVAQLLHLG
                     PYATERASIERLHAAVAEAGLRLRGRHHELYLGNPQASAPGRLRTILRHPVE"
     gene            complement(4742..5140)
                     /gene="mscL"
                     /locus_tag="VHM48_02895"
     CDS             complement(4742..5140)
                     /gene="mscL"
                     /locus_tag="VHM48_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX2754380.1"
                     /translation="MASGFREFLLKTNALALAFGVIIGTALGTVVNSLVNDIIMPPIG
                     ALLGGVDFGQLKFVLTQAVGGDPATEVAIRWGAFVNAIIAFIVIAFVVWQISKMFIKE
                     PAPAEVKTCPFCREANALDATRCRACTSAI"
     gene            complement(5140..>5559)
                     /locus_tag="VHM48_02900"
     CDS             complement(5140..>5559)
                     /locus_tag="VHM48_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEX2754381.1"
                     /translation="AGGPGMKVEDVEGIGPTFAAKLIEAGVTTTDALLDRGGTSAGRD
                     RLSEATGISEKLLLEWVNHADLMRIDGVGSEYADLLEAAGVDSCAELVQRNAANLAIT
                     FRELDAARPDWIRQVPGEATIAGWIEQAGGFANVVSH"
BASE COUNT          841 a         2032 c         1933 g          753 t
ORIGIN      
        1 tgcgcgatgc tcctcgtccg ccgaccggcc gacttcgacg tgatcgtcac cgagaacctc
       61 ttcggcgaca tcctgtccga cgaggcggcg gtcctggcgg ggagcctcgg gatgctgccc
      121 tcggcctcgc tcggcgaacg acggacggcc cacggcgcgt tcggcctgta cgagccgatc
      181 cacggatcgg cgccggacat cgccgggcgc gacctcgcca acccgatcgg gacgatcctg
      241 tcggccgcga tgctcctccg cctgtcgctg ggccgggagg atgccgcggc ggcgatcgag
      301 gccgccgtca gccagaccct cgaccacggc ttccggaccg ccgatctcgt tccgccgagc
      361 ggcgacgaga cgggcttgcg ccgcgtgggt acggtcggga tgacggccgc gatcgtcgaa
      421 cggatcgcga ttgcggaggc tgcccgggcg accgcggcag cccgcacgtg atcccgccga
      481 ccgccaccgc acgcaggacc ttcccccgat gacgaccccg accgaccttc cggtcaccct
      541 ctacgacacg acgctccgcg acggcactca gggcgagaac atcacgctca gcctcgccga
      601 caagctccgg atcgcccgga tgctcgacga gtacgggatg ccgtacatcg agggcggctg
      661 gccgggctcg aacccgaagg acatcgagtt cttcaaggcc gctcgctcga tgacgtggca
      721 gaccgcgaag ctggcggcgt tcggctcgac ccgccatcga tccaacaccg ccgagaacga
      781 tcccaacctg cgcgagctgg tcgccgccga gacgccggtc gtgacgatct tcggcaagag
      841 ctggctcctc cacgtggtgg aggtcctggg ggccacgccg caggagaacc tcgacatggt
      901 cgcggactct gtccggttca tcgtcgagcg tgggcgcgag ctcgtctacg acgccgagca
      961 cttcttcgac ggctatcgtg acgaccgcga ctacgccctc gcgacgttgc gggcggcccg
     1021 ccaggccggc gcccggacgc tggtcctgtg cgatacgaac ggcgggacgc tgaccgacga
     1081 gctggtccgg atcgtcaccg actgccggtc gtcgctggag ggcgacccgg gagccgacgg
     1141 cacgccgatc acgtggggca tccacaccca caacgacgcc gagctcgcgg tcgccaactc
     1201 gatggccgcg gtggcggtcg gcgtccgcca cgtccaggcc acgatcaacg gctacggcga
     1261 gcgggccggc aacgccaaca tggtctcgat cctcgccaac ctcgccctca agaccgccca
     1321 cgagctggtg cccgccggcg gcgggacgct gaccggcctg acggagctca gccggtcggt
     1381 ggccgagatc gccaacatca accccaacga ctggcagccc tacgtcggcc ggtccgcgtt
     1441 cgcccacaag ggcggcgtcc acggcgctgc ggtcgccaag gtcgagcgga gctaccagca
     1501 catcgagccg acagccgtcg gcaacgtcgg ccggctcgtc gtctcggaac ttggcggtcg
     1561 ggccaacacc cgcatccggg ccgagcagct tggccaccag ctcgagggtg tcatcgatcc
     1621 gaaggccctg agcgagctcg tgaaacagct cgagagcgag ggcctcgcgt tcgagggcgc
     1681 cgaggcgtcg ttcgagctgt tgatccgccg ccaccaggcc gactacgtcg cgccgttccg
     1741 gatcgtcgat tacacctgcc tggtcgagca gcgctcgggg caggagctcc tgtccgaggc
     1801 gacggtcaag gtccaggtcg acggcgagat cctccatacc gcggccgacg gcaacggccc
     1861 ggtcaacgcc ctcgatgcgg cgctccgcaa ggcgctccgc gcgttctacc cgcagctcga
     1921 cgacgtccac ctgtacgact acaaggtccg gatcctcgac ggcgactcgg cgacggccgc
     1981 ccggacgcgg gtgatcatcg actcgtccga cggggccctg gagtggtcca cgatgggcag
     2041 cgacacgaac atcatcgcgg cctcggcgaa ggccctcgcc gactcgctcg aatacgcgat
     2101 ctggaagtcc ggggcggagc tcgtccgccg cgacgaacga catttcacga cgacgccggc
     2161 cagccccgcg gcgacgtcac ggaccgggag gtaaccatgg ccgccgacac gacccccgcc
     2221 gcgaccgcgc cgacgggcga cgccgccgac acgctccacc tcacccgctg gacggtgacg
     2281 tccggctcga acgcgaacag ccgcggcgcg gtcgtgatct ccgccggcga tcaccagtgg
     2341 caggcgtcgg ccgaaggcaa cggggcggtc gatgccctgt tccgggcggt cgacaaggcg
     2401 ctcgccgggg tactcacggg gcaaccgcgc ctgctctcct acgacgtcca cgcggtcgcc
     2461 gagggccctg acgccgccgg caaggtgact gttgcgatcg cgccgccggc ggacgcctcg
     2521 ggaccccggg ccagcggtcg ctatgtcggc gacatcacct cgacgaacat catcgcggcc
     2581 tcgatcgagg cgtacgtcga cgcggtcaac gcgctcctcg cggaggaaca ctggcagggc
     2641 gcgaccgaga cggcgggcaa tcgccgccga gcccgggtga gcgagcccgc ggcgaaggcc
     2701 cagcgggccg agctcgacga agaggcgggc cggatcgaca cgaccgactg gttcaaccac
     2761 tgacggatag cgtcgcggag cctccccgga gctcagcgag atgagccccg cctcggacaa
     2821 tggtgagcga gtgacagttc cgcgctgagg gtgtcagctc ccgtccggac gacacgcgtt
     2881 tgcccatacg tcagccaagc gcgcactgga ggcggcctcc aacgcctgtc ggcgcgaatc
     2941 cttcccatcc gcccgcggcc ctgtcgtatg ggacggaatc gggtccctcc gggtccggcg
     3001 ctcgaggcaa cgccgccaaa tgccacccgg gctggcgtat ggcaacgacg acgtcgaccc
     3061 gcctgcggat cggccacact tcggcgatga agagcgagac gcaggtcgtc taccagggtg
     3121 agccgggcgc cttctcggag gaggccgtcc tctcgttctt cggggagtct cgcgagcgga
     3181 ccgccgtgcc gacatggcgg gccgtcttcg aggcggtcgc cgacggttcg gccggcggcg
     3241 gagtgatcgc gatcgagagc tcgctggcgg ggacgatccg cgagacctac gacctgctcg
     3301 ccgagttcta cgaccgcgac gtccggatcg tcggcgaggt gaccgttccc gtccggctgg
     3361 ccctcctcgc cctgcccggc cagagcctcg actggatcga gcgggtctac agccacggcc
     3421 aggcgctcgt ccaggccgac gccttcctgc gctcccgtga gtggcaggtg atgacgacct
     3481 acaacaccgc cggcgcggcc cggatgatcg ccgagcgtca tgaacggcat gccgcggccg
     3541 tcgcctcgcc gcgggtggcc gccctctacg ggctggagat cctcgccgcg gacatccagt
     3601 cgggcgacga gaaccggacc cggttcgcgg tcctgtcgcg ccgtggaccg gagggagccc
     3661 acggagccgc cccggtcgac ccggtcggcc ctcggctgac gacgctcgtt ttcgcggtcc
     3721 gcaacctccc gggctcgctc caccgctgcc tcggcgcgtt cgccgcgcga ggcgtcaacc
     3781 tctcctcgct cgagtctcgg ccggcgcgac gggcccgttg ggagtacgtg ttctgggtcg
     3841 atgtcgacgc cgccgcggac gagccggcgt gtgccgcggc cctcgacgat ctccgcgcgg
     3901 agaccgagat ggtcaaggtc ctcgggtcgt acccgcgcgc ccgcgaggac tagcgatccc
     3961 gccgccgggc ccgcgcgagc cctcgcatca ctccaccggg tggcgcagga tcgtccggag
     4021 acgcccgggc gcgctcgcct ggggattgcc gagatagagc tcgtgatggc ggccccggag
     4081 ccggagcccc gcctcggcga ccgcggcgtg gaggcgctcg atcgaggcgc gctcggtcgc
     4141 gtacgggccg aggtggagga gctgggcgac ccgaccctcg gcgagcgcct cgatccgcag
     4201 gctcggcgcg aacgcctcgc cgagcttcgc ccggccggcg cccacggccc gctcgagctc
     4261 gtcgtcggtg gcctcgtcgg gcagcgcgat cagcagcgtc cagcgccacg tcccccgctc
     4321 gccggcgccg ccgaacaccg cgtcgaggtc ggtcgaacca tcgctggtcc accacagccc
     4381 ttcgagcggc ccgaccttcg tgacgacgcc gcgcgccttg agcccgaacc ggaccttgta
     4441 cgcggtcgtg tagagcccgg gcatccgcgg cacgaaggca tcggggccgg gtgcgccatc
     4501 gccgtcgatc atgaccgctc gcgtcgccgg caggtcgagg aaccggacga tccccgcgcc
     4561 gccgcggagc tccgcgtcgc ggtcgacggg gtgttcgaag atgaccggac cggccgtcac
     4621 gaccatggcg ccacggtaga acggccgcac ggcgatcgcc agagccgaac ggcctcgact
     4681 ccgtgcccga cacgacgaag gccgggcggg cgcccgggcg tcgggtcgac cggcgggccg
     4741 gtcagatggc gctggtgcag gctcggcacc tggtggcgtc gagcgcgttc gcctcgcggc
     4801 agaacgggca ggtcttcacc tcggcgggcg ccggctcctt gatgaacatc ttcgagatct
     4861 gccagacgac gaaggcgatc acgatgaagg cgatgatcgc gttcacgaat gcgccccagc
     4921 ggatcgcgac ctcggtcgcc ggatcaccgc cgacggcttg cgtcaagacg aacttcagct
     4981 ggccgaagtc gacgccgccg aggagggcgc cgatcggcgg catgatgatg tcgttgacga
     5041 gcgagttgac gaccgtcccg agggcggtcc cgatgatgac gccgaacgcc agggcgaggg
     5101 cgttggtctt gagcaggaac tcgcgaaacc cgctggccat cagtgactga cgacgttcgc
     5161 gaagccgccg gcctgctcga tccagcccgc gatcgtcgcc tcgcccggga cctggcggat
     5221 ccagtccggg cgggcggcgt cgagctcccg gaaggtgatc gcgaggttgg cggcgttgcg
     5281 ctggaccagc tctgcgcacg agtcgacgcc ggccgcctcg agcaggtccg cgtactcgga
     5341 gccgacgcca tcgatccgca tgagatcggc gtggttgacc cactcgagaa gcagcttctc
     5401 ggagatcccg gtcgcttccg acaggcggtc ccggccggct gaagtcccgc ctcggtcgag
     5461 cagcgcgtcc gtcgtcgtga ccccggcttc gatgagcttg gcggcgaatg tcgggccgat
     5521 gccctcgacg tcctcgacct tcatccccgg tcctccagc
//
LOCUS       DATAIW010000086         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_63523, whole genome shotgun sequence.
ACCESSION   DATAIW010000086 DATAIW010000000
VERSION     DATAIW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63523"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="VHM48_02905"
     CDS             complement(<1..2069)
                     /locus_tag="VHM48_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2754382.1"
                     /translation="MHADHETPRDHDLPAGTAPAAHDQQAGTAQTAPAAHDNGHDKHA
                     VAHDKHTGHSVAMFRDKFWLSLLLTIPVLIWSRDPQAWLRYMAPEFPGSDWIPAVLGS
                     VVFLYGGLVFVRGAVGELRIRQPGMMTLISLAIVVAFGTSWSGTLGLFEVEIWWELAT
                     LITIMLLGHWLEMRSIAQARGALTALAELLPDTAERVTAHGTETVPRAELAVGDVILV
                     RPGARIPADGVVDEGSADVDESMITGESQAVAKERGDRVVAGTVAAGGSLRVRVAAVG
                     EETALSGIMRMVAAAQASASRAQALADRAAAILFYVALAAGGLTLAYWWLWGDPEGAL
                     VRTATVLVIACPHALGLAIPLVIAISTSLGARNGLLVKSRIALERARELDVIIFDKTG
                     TLTMGRPALAAISAAGGMDEAGVLRLAASVEADSEHPLARAIVAAASERGGEVPTATA
                     FEALTGRGARATVEGHVVAVGGPRLLADLGLEPDPASEVWARDGRTVLHVVADGAVVG
                     ALAIEDEIRPESAQAIERLHAMGITVAMITGDAQAVADSVARRLGIDEVAAQVLPEDK
                     ADAVRRFQQGGRKVAMVGDGVNDAPALATADVGIAIGAGTDVAVESAGIVLVRSDPRD
                     VVGAIELSRATYAKMIQNLVWATAYNLVAIPVAAGVLVPWGIDLPMAVGAIAMSLSTI
                     IVAANAQL"
     gene            complement(2059..3189)
                     /locus_tag="VHM48_02910"
     CDS             complement(2059..3189)
                     /locus_tag="VHM48_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2754383.1"
                     /translation="MRWLDPGRLHWRLLAGNLVVAAAGAITVAIAVSLAAPVAFEHAM
                     GSQGAMAGMGTMMSSVVTTAFGDAVGSALVLGVGAATLIAIVVAAIISTRLARPISSL
                     ADASREVAGGDYAQRVPVGSGELGDLAASFNDMAASLDATERRRHELIGDVAHELRTP
                     IASLRGYVEGLEEGVFTPGPDAWRVLGDQASRLARLVDDLSDLWRAEGRDIALVSEDL
                     DGPTLVVDAIQRHRAAAAARSIELDAEADEPVRLRADRARVAQVLDNLVGNAIRYTPE
                     RGRVRVRLARRGNVAQVSVTDTGPGLAQGQLAQVFERFYRVDSSRSRDVGGSGLGLAI
                     AKALTEAMGGRIWATSPGTGLGATFWVELPAVQLPIERPHAR"
     gene            complement(3186..3893)
                     /locus_tag="VHM48_02915"
     CDS             complement(3186..3893)
                     /locus_tag="VHM48_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2754384.1"
                     /translation="MANTGSRTRVLVVDDESSIRDIVRGFLERDGMTVVEAADGPSAV
                     EIAREAAPDVVVLDLMLPGFDGLEVLRRIRTFADPYVLLLTARSEEVDRIVGLTVGAD
                     DYLTKPFSPRELVARVHALMRRRRPGTRDAGDVLQAGDLLVDPRRRTVARAGEPVDLT
                     VLEFDLLAALVREPGMVFTRQQLLDAVWGVDFFGDDHVLDVHVANLRRKLGDDASGAR
                     YVETVRGVGFRCPEERR"
     gene            complement(3973..>4227)
                     /locus_tag="VHM48_02920"
     CDS             complement(3973..>4227)
                     /locus_tag="VHM48_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2754385.1"
                     /translation="VVTVQAGETITFEVTTMGPVVHEFMVGPAADVAADKAGTPEIAD
                     LGMMQTRSLTYTFTGTGPFAFACHVPGHYEAGMSGTIVVQ"
BASE COUNT          632 a         1518 c         1490 g          587 t
ORIGIN      
        1 agctgggcgt tggcggcgac gatgatcgtg gagaggctca tcgcgatcgc gccgacggcc
       61 atgggcaggt cgatgcccca cggcaccagc acccccgccg ccaccgggat cgccaccagg
      121 ttgtaggcgg tcgcccagac gaggttctgg atcatcttcg cgtaggtcgc ccgggatagc
      181 tcgatcgcgc cgaccacgtc ccgtgggtcg ctgcggacca ggacgatgcc ggccgattcg
      241 acggcaacgt ccgtcccggc gccgatcgcg atccccacat cggcggtcgc gagggccggg
      301 gcatcgttca cgccgtcgcc taccatggcg accttgcggc cgccctgctg gaaccggcgg
      361 acggcgtcgg ccttgtcctc gggcaggacc tgggccgcca cctcgtcgat cccgagccgt
      421 cgcgcaaccg agtccgcgac cgcctgggcg tcgccggtga tcatggcgac cgtgatgccc
      481 atcgcgtgga ggcgttcgat cgcctgggca gactccggcc ggatctcgtc ctcgatcgcg
      541 agcgcgccga cgacggcgcc gtcggccacc acgtggagca cggtgcgacc atcgcgggcc
      601 cagacctcgc ttgccgggtc gggctccagg ccgaggtcgg cgagcaggcg cggcccgccc
      661 acggccacga cgtgcccttc tactgttgct cgtgccccgc ggcccgtgag cgcctcgaag
      721 gccgtggcgg tggggacttc gcccccgcgc tccgacgcgg cggcgacgat cgcccgggcg
      781 agcggatgct cggagtcggc ctccaccgac gccgccaggc gcaggacgcc ggcttcgtcc
      841 atcccgccgg ccgcggagat agccgccagg gccggacgcc ccattgtcag cgtcccggtt
      901 ttgtcgaaga tgatcacgtc gagctcgcgt gcccgctcga gcgcgatgcg gctcttcacg
      961 aggaggccgt tccgcgcgcc gagcgaggtc gagattgcga tcacgagtgg gatggcaagc
     1021 ccgagggcgt gggggcaggc gatgaccagg accgtcgcgg tgcggacgag ggccccttcg
     1081 gggtcgcccc agagccacca gtaggcgagc gtcagcccgc cggcggcgag ggcgacgtag
     1141 aagaggatcg cggcggcgcg atcggccagc gcctgcgcgc gcgaggcgga ggcctgcgcg
     1201 gccgcgacca ttcgcatgat ccccgaaagc gcagtttcct caccgacggc cgctactcga
     1261 acccggaggc tcccgccagc cgcgacggtg ccggcgacga cccgatcgcc gcgctccttg
     1321 gcgacggcct gcgactcgcc cgtgatcatc gactcgtcga catcggcgga tccctcgtcg
     1381 acaaccccgt cggccggaat ccgggcgccc ggccggacga ggatgacgtc gccgacggcg
     1441 agctccgcgc ggggaaccgt ctccgtgccg tgcgcggtga cgcgctcggc cgtgtccggc
     1501 agcagctcgg cgagcgccgt gagggctccc cgcgcctggg cgatcgagcg catctccagc
     1561 cagtgaccga gcagcatgat cgtgatgagc gtggcgagtt cccaccagat ctcgacctcg
     1621 aacagtccga gcgtgccgga ccagctcgtg ccgaacgcga cgacgatcgc gagcgagatg
     1681 agggtcatca tgccgggctg tcggatccga agctcgccga cggctcctcg aacgaacacg
     1741 agcccgccgt acaggaagac caccgagccc agtaccgccg ggatccagtc ggagcccggg
     1801 aattccgggg ccatgtacct gagccacgcc tgcgggtcac ggctccagat gaggaccggg
     1861 atcgtcagga gcaggctgag ccagaacttg tcgcggaaca tcgcgacgga gtgaccggtg
     1921 tgcttgtcgt gcgccactgc atgcttgtcg tggccgttgt cgtgggcggc aggcgccgtc
     1981 tgggccgtcc ccgcttgctg gtcgtgcgcg gcgggggccg tgcctgcggg caggtcgtgg
     2041 tcgcgcggcg tttcgtggtc agcgtgcatg gggccgctca attggcaatt ggactgccgg
     2101 cagctcaacc cagaacgtcg ctcccagccc ggttccaggg ctggtggccc agatccggcc
     2161 gcccatggcc tcggtgagcg cctttgcgat cgcgaggccg agcccgctgc caccgacgtc
     2221 tcgagacctc gacgagtcaa cccggtagaa gcgttcgaag acctgggcaa gctgcccctg
     2281 cgcgagccct ggtcccgtgt ccgtgaccga gacctgggcg acgttgccac ggcgcgcgag
     2341 gcggacgcgg acacgccctc gttccggcgt gtagcggatg gcgttgccga cgaggttgtc
     2401 gaggacctgg gcgacgcgag cccgatctgc ccgcaggcga accggctcgt ccgcctcggc
     2461 gtccagctcg atcgaccggg cggcggcggc ggcgcgatgg cgctggatcg catccacgac
     2521 gagggtcggc ccgtccagat cctccgagac gagggcaatg tcacgcccct cggcgcgcca
     2581 gaggtccgag aggtcgtcga cgaggcgcgc caaccggctc gcctggtccc cgaggacgcg
     2641 ccacgcatcc ggcccgggag tgaacacgcc ctcctcgaga ccttccacgt acccgcgcag
     2701 cgatgcgatc ggcgttcgca gctcgtgcgc cacgtcgccg atcagctcgt gtcgtcggcg
     2761 ttccgtggcg tcgagcgacg ccgccatgtc attgaacgat gccgcgaggt cccccagctc
     2821 gccgctgccg acggggacgc gctgcgcata gtcgcccccg gcgacctccc ggctcgcgtc
     2881 ggcgaggctg ctgatcgggc gagcaagccg ggtcgagatg attgcggcga ccacgatggc
     2941 gatcagggtg gccgccccca ccccgaggac gagcgccgac ccaacggcat ccccgaacgc
     3001 ggtcgtgacc acggagctca tcatcgtgcc catccctgcc atggccccct ggctgcccat
     3061 cgcgtgctcg aacgcgaccg gggcggccaa gctcacggcg atcgcgacgg tgatcgcgcc
     3121 ggcagcggcg acgaccaggt tgccggccag gagccgccag tggagccggc ccgggtcgag
     3181 ccacctcatc ggcgttcctc cgggcaacga aaccccacgc ctcggacggt ctcgacgtac
     3241 cgcgcacccg acgcatcgtc gccgagcttg cgtcgaaggt tggcgacgtg gacgtcgagg
     3301 acgtggtcgt cgccgaagaa gtccacgccc cagaccgcgt cgagcagctg ctgccgggtg
     3361 aagaccatgc ccggctcgcg aacgagcgca gccaggaggt cgaactcgag cacggtcagg
     3421 tcgacgggtt cgccagcccg agcgacggtc cgacgtcgcg ggtcgacgag gagatcgccg
     3481 gcctgcagga cgtctcccgc gtcacgggtt ccaggtcggc gacgccgcat gagggcgtgc
     3541 acccgggcga cgagctcgcg cggcgagaac ggcttggtca ggtagtcgtc cgcgccgacc
     3601 gtcaggccga cgatccggtc gacctcctcg ctccgcgccg tgaggagcag gacgtacggg
     3661 tccgcgaagg tccggattct gcggaggacc tcgaggccgt cgaaaccggg aagcatcagg
     3721 tcgagcacca cgacgtcggg cgccgcctcc ctggcgatct ccacggcaga cggaccgtcg
     3781 gcggcctcaa cgaccgtcat gccatctcgc tcgaggaacc cgcgaacgat gtcccggatc
     3841 gacgactcgt cgtcgacgac gagcacgcgt gtccgggacc ctgtgttcgc catgcccacc
     3901 tcggattcca cccgggcgat cccaagcccg aggccggcgt ccattcccag gagccccggg
     3961 ctcgtgcgtt ccctactgga cgacgatcgt cccgctcatg ccggcctcgt agtgtccggg
     4021 aacgtggcac gcgaaggcaa acggacccgt gcccgtgaag gtatacgtga gggacctggt
     4081 ctgcatcatg ccgagatccg cgatctcggg cgtcccggcc ttgtcggcgg ccacgtcggc
     4141 cgcgggtccg accatgaact catgcacgac ggggcccatc gttgtgacct cgaaggtgat
     4201 cgtctcgccg gcctggaccg tcacgac
//
LOCUS       DATAIW010000087         6317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_63698, whole genome shotgun sequence.
ACCESSION   DATAIW010000087 DATAIW010000000
VERSION     DATAIW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63698"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHM48_02925"
     CDS             complement(<1..204)
                     /locus_tag="VHM48_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754386.1"
                     /translation="MAPDPVQSLANLRLERDAVLLYEALAAIEKDPQRGAAFHRIAGN
                     ERRHAEIWAGKLREAGVDVPPAPT"
     gene            239..1249
                     /locus_tag="VHM48_02930"
     CDS             239..1249
                     /locus_tag="VHM48_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754387.1"
                     /translation="MTARDAPPLRYLTAADVSAAMPPLSERLGLAERTLTALVDDSEL
                     PPKIGVHPRPDGSFAHAMPAWLRGADPGGADDLLGIKWIAGFPANRQREQPAIHGLVV
                     LADPTTGIPTAILDAGPITAERTAAISGVAIARFAPDVVGRAPRVALLGTGVQGRSHL
                     AVLGHVLPGVELTVFDRHPDRATALAAVAEQTPGIRSARSASTARDATRSADIVVTTA
                     SFAPADQRQPLTDDWLTRDALVVAVDYATYVAASVARNAALFLVDQREQFLANRDAGH
                     FDGYPDPGATLGEAIIAGTPRPHRGRVLVTHLGVGLADLVFGNAIVRRAIGRGLGTVL
                     PR"
     gene            1388..4063
                     /locus_tag="VHM48_02935"
     CDS             1388..4063
                     /locus_tag="VHM48_02935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX2754388.1"
                     /translation="MHRLVGLIKRLVWLVVILAVVLVAVGTGLIGTLTLRGFPTTHGS
                     LAITGLDDTVRVTRDAAGIAWIEAETSHDLFVAQGYVHAQERMWQMEVWRHISSGRLS
                     ELFGPSTRTEDRFIRTLGWRQAAQRDLDALSPDAQAVVDAYAAGVNDWIADHNGSLSL
                     PFVVAGLKAGLDGGIGGYKLERWTALDTLAWQKVQAWQLGGNFDSEIFRMLADAKLGD
                     PARTDELFPAYDPKMPVITPSGLKGSGGAGNQAVNLTGDAPNPGVAAAAEPSQSPSTA
                     IGDPTAWRDLAAAGGRILEIAGLDGADGIAGNHQVGSNDFVVGRSKTKTKTALLANDP
                     HLGIGMPSVWFMNGLRCRAVSKDCPFDVAGVSFPGVPGIVLGHNAKIAWGATNVDPDV
                     EDLFSIDLDPANPANYLVAGQAVPFDVRHEVIKVAGGADVQLDVRTTRFGPIVTDVDS
                     RLKGAPLLALSWTGIDDVDGTLEAIFHLNTASNFEEFHAAFKTYGAPSQNFVYADTAG
                     HIGYVLPGRIPIRSDPNDHGERIRSGSDGKHDWASTIPTDDLPWQLDPAGGVIVTANN
                     AAVDAKYPFFIAREWDPGYRAKRITDLLAEAAKAGGVTTDTLRAIQFDSRVLRAEVIA
                     PFTGSVTTTTQDGALIAQRIRDWSTFDCPVDGPASLGCAAYLAFEYRLVRGLLDDDLG
                     DLAREYVGGGASWQATIALLDQPDSAWWDDKTTTDRVETRDDIVAAALDEAGRELRAA
                     YGDPNKWTWGTLHTAKFSEATLGSSGIGPLEWYFDKGPYPAPGASGAVNNTYFRPSRA
                     YRDPDDATYKPVGIDGVFAVTNLPSYRLSIDMADLDGAQIVQTTGQSGNPFDRHYGDL
                     IDEWLTGQAVPLPFTQSAVSDAAIETLQLVPRTGG"
     gene            complement(4275..4613)
                     /locus_tag="VHM48_02940"
     CDS             complement(4275..4613)
                     /locus_tag="VHM48_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2754389.1"
                     /translation="MRRLDLVGVDEVADGEMRIVYVDGTDQVLLINQGGEFTATQGIC
                     SHEYFELDKGFLTAGTLTCALHLSRFDLSSGEPIDPPAELPLEIYPVVIEDGRVLIEV
                     PDGPLPISEQ"
     gene            complement(4639..6066)
                     /locus_tag="VHM48_02945"
     CDS             complement(4639..6066)
                     /locus_tag="VHM48_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX2754390.1"
                     /translation="MIDATPEATPATPAAPARPARAPRRSPNELTFTLEEATVAAHAV
                     AEPDWLTADRSAAFARYRALPIETNQLYTTYVDLRTADLTAVTPWESPAGAPVGGASQ
                     PLPDGAAGFAAFTEDRVEALLLDEATRAAGVVLDSLAGLIARDEALARRLLGDETSLP
                     LDDRLAQVTRAGWNQGVVVHVPAGVRLERPIVVRWQAGEPGRAMLTRTILDLGKGASA
                     SIVEELEASSGAHDRTAAQALFTGSLEVHLAAGAELAIASIQNLATGTVAFQHRQAEI
                     GEGASLHWALAQLGGRLVRSRVDNRLSGNRSSVEQVEIVFGADDQLFDLTSYTRHIGR
                     DTTGNLLSKAALMDATRSYMKGLIIIEPTAVGTDSFLGEFGMNLSKQARAVAIPSLEI
                     DQPDCRRAAHSSAVGPIDETQLFYLESRGIDPEEARKFIVLGFLEPVVARVPLVSAQN
                     TLRELLEAKWDAGAAHAAAATSSAA"
     gene            complement(6063..>6317)
                     /locus_tag="VHM48_02950"
     CDS             complement(6063..>6317)
                     /locus_tag="VHM48_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX2754391.1"
                     /translation="DISRSDTYPYIDIQEDDTTMTHEATVGKVSAEQVFYLMSRGLTE
                     NEAQNLIVQGFLEVFTKELPMEYAIEFNRLVKLEMEGSLG"
BASE COUNT          918 a         2266 c         2220 g          913 t
ORIGIN      
        1 ggtgggagcg ggcggcacat ccaccccggc ctcacggagc tttccggccc agatctcggc
       61 gtggcgccgc tcgttgcccg cgatccggtg aaacgccgcg ccccgctggg ggtctttctc
      121 gatcgcggcc agcgcctcat agaggaggac cgcgtcgcgt tcgagacgca ggttggcaag
      181 gctctggacg ggatcggggg ccacgagccc atgctacccg gagggtagac tcgggacgat
      241 gacggcccgg gacgcacccc cgctccgata cctgacggcg gccgacgtca gcgccgcgat
      301 gccgccgctc agcgagcgac tcggtctcgc cgagcggacg ctgacggccc tcgtcgacga
      361 ttccgagctg ccgccgaaga tcggggtcca tccgcggccc gacggctcgt ttgcccacgc
      421 catgccggcc tggcttcgcg gcgccgatcc cggcggggcg gacgatctcc tcgggatcaa
      481 gtggatcgcc ggcttccctg ccaaccgcca gcgcgagcag ccggcgatcc atggcctggt
      541 cgtcctggcc gacccgacga ccggcatccc gaccgcgatc ctcgatgccg gcccgatcac
      601 ggccgagcgg accgccgcga tcagcggcgt ggcgatcgcc cggttcgcgc cggatgtcgt
      661 cgggcgggct cctcgcgtcg ctctcctcgg gaccggcgtc cagggtcgaa gccatctcgc
      721 ggtgctcggc catgtcctgc cgggcgtcga gctgacggtc ttcgatcgac accccgatcg
      781 ggccacggcc ctcgctgcgg tcgcggagca gacgcccggg atccgatccg ctcgatcggc
      841 ctcgaccgcc cgcgatgcga cccgatccgc cgacatcgtc gtcaccacgg cctcgttcgc
      901 gccggccgat cagcgccagc ccctgaccga cgactggctg acacgcgatg ccctggtggt
      961 cgccgtcgac tacgcgacct atgtcgccgc ctcggtcgcc cgcaacgcgg ccctcttcct
     1021 cgtggatcag cgcgagcagt tcctcgccaa tcgcgacgcg ggtcatttcg acggctaccc
     1081 ggaccccggc gcgactctcg gcgaggcgat catcgccggg acgccgcggc cgcatcgcgg
     1141 ccgcgtgctc gtgacccacc tcggcgtcgg gctggccgac ctcgtgttcg ggaacgccat
     1201 cgtccgtcga gcgatcggtc ggggcctcgg aaccgtgctc ccgcgctgac accggtcgcg
     1261 acgggccgac ccgccggtcg agcctgcgcg acgagcctga cggggcgacc gtcgagcggg
     1321 cacactggcg aggccagcac gggcgcgtac gaccgggctg agggcggatc ccggggaggg
     1381 ttcgagcgtg catcgtctgg tcgggttgat caagcgcctc gtctggcttg tcgtgatcct
     1441 cgccgtggtc ctcgtcgcgg tcgggacagg cctgatcggc acgctgacgc tgcgcggatt
     1501 cccgacgacc cacggcagcc tcgcgatcac cggcctggac gacaccgtcc gggtgacccg
     1561 cgacgcggca ggcatcgcct ggatcgaggc cgagacgagc cacgatctgt tcgtcgccca
     1621 gggctacgtc cacgcccagg agcggatgtg gcagatggag gtctggcgcc atatctcctc
     1681 gggtcgactc tccgagctgt tcggcccgag cacgcggacc gaggaccggt tcatccggac
     1741 gctcggctgg cgccaggcgg cccagcgcga cctcgacgcc ctctcgcctg acgcgcaggc
     1801 cgtcgtcgac gcgtacgcgg ccggggtcaa cgactggatc gccgatcaca acggatcgct
     1861 gtccctgccg ttcgtcgttg ccgggctcaa ggccgggctc gacggcggca tcggcggcta
     1921 caagctcgaa cggtggaccg cgctcgacac gctcgcctgg cagaaggtcc aggcctggca
     1981 gctcggcggc aacttcgaca gcgagatctt ccggatgctg gccgacgcga agctcggcga
     2041 cccggcccgg accgatgagc tcttcccggc ctacgacccg aagatgccgg tcatcacccc
     2101 gagcggcctg aagggctcgg gcggcgcggg caaccaggcc gtgaacctga ccggcgacgc
     2161 tccgaacccg ggcgtggccg cggcggccga gccgagccaa tccccgtcca ccgcgatcgg
     2221 cgacccgacg gcctggcgcg atctcgccgc ggccggcggc cggatcctcg agatcgccgg
     2281 gctcgacggc gccgacggga tcgcgggcaa ccaccaggtg ggctcgaacg acttcgtggt
     2341 cggccggtcg aagacgaaga ccaagaccgc cctcctcgcc aacgacccgc acctcgggat
     2401 cgggatgccg tcggtctggt tcatgaacgg gctccgttgc cgggccgtca gcaaggattg
     2461 cccgttcgac gtcgccgggg tctcgttccc gggcgtgccg gggatcgtcc tcggccacaa
     2521 cgcgaagatc gcctggggcg ccacgaacgt cgaccccgac gtcgaggacc tgttctcgat
     2581 cgacctggac ccggccaacc ccgccaacta cctcgtcgcc ggccaggcgg tcccattcga
     2641 cgtgcggcac gaggtgatca aggtcgccgg cggggcggac gtccagctcg acgtccggac
     2701 gacccgcttc ggcccgatcg tgaccgacgt cgacagccgc ctgaagggcg cgccgctcct
     2761 cgccctgagc tggactggca tcgacgacgt ggacggcacg ctcgaggcga tcttccacct
     2821 caacaccgcc tcgaacttcg aggagttcca cgccgcgttc aagacatacg gcgcgccgtc
     2881 ccagaacttc gtctacgccg acacggccgg ccacatcggc tacgtcctgc ccgggcgaat
     2941 cccgatccgg tccgacccga acgaccacgg ggagcggatc cgctcgggca gcgacggcaa
     3001 gcacgattgg gcctcgacga tcccgaccga cgacctgccc tggcagctcg acccggcagg
     3061 cggcgtgatc gtgaccgcca acaacgccgc agtcgacgcg aagtacccgt tcttcatcgc
     3121 ccgggagtgg gatccggggt accgggccaa gcggatcacg gaccttctcg cggaagcggc
     3181 gaaggccggc ggcgtcacga cggacacgct ccgcgccatc cagttcgaca gccgcgtcct
     3241 gcgggcggag gtgatcgcgc cgttcacggg gagcgtcacc acgaccacgc aggacggtgc
     3301 gctcatcgcg cagcggatcc gggactggtc cacgttcgac tgcccggtgg acggacccgc
     3361 gagcctcggc tgcgccgcct acctcgcatt cgaataccgg ctcgttcgcg gcctgctcga
     3421 tgacgacctc ggcgacctgg cccgcgagta cgtcggcggt ggcgcgtcct ggcaggcgac
     3481 gatcgccctg ctcgaccagc cggactcggc gtggtgggac gacaagacga cgaccgaccg
     3541 ggtcgagacg cgcgacgaca tcgtggccgc ggcgctcgac gaggccgggc gcgagctccg
     3601 cgccgcctac ggggatccga acaagtggac ctggggcacg ctccacaccg ccaagttcag
     3661 cgaggcgacg ctcgggtcgt ccgggatcgg gccgctcgag tggtacttcg acaaaggccc
     3721 ctaccccgcc ccgggcgcct cgggcgcggt caacaacacc tacttccgcc cttcccgcgc
     3781 ctaccgggat cccgatgatg cgacctacaa gccggtcggc atcgacggcg tcttcgcggt
     3841 caccaacctg ccgtcctacc ggttgtcgat cgacatggcc gacctcgacg gggcgcagat
     3901 cgtgcagacg accggccaga gcgggaaccc gttcgaccgc cactacggcg acctgatcga
     3961 cgagtggctg acgggtcagg cggttccgct gccgttcacc cagtccgccg tcagcgatgc
     4021 cgcgatcgag acgctccagc tggtgccacg aaccggcggg taggcgccac gcggctgcgc
     4081 tgggacgcgt gccgcgcgct cggtgcgcgt tgcgacgaac ggcgggccca ccggacgccg
     4141 ttgcgcgcgc ctgacgcgca gttcgacgca ggaaccgaag attcgggcct caatggacct
     4201 tccctccagc cctcgttcgc cggaatgcgc gtcgggcgcg caaccgatgc cgtgcccgac
     4261 ggcccgcgcg acccctactg ctcgctgatc gggagcggcc cgtccgggac ctcgatcaag
     4321 acgcgaccgt cctcgatcac gaccgggtag atctccaggg ggagctcggc gggggggtcg
     4381 atcggctccc cgctcgagag gtcgaaccgc gacaggtgaa gggcgcacgt cagggtgccg
     4441 gcggtcagga agcccttgtc gagctcgaag tactcgtggc tgcagatgcc ctgggtcgcg
     4501 gtgaactcgc caccctgatt gatcaggagg acctggtccg tgccgtcgac gtagacgatc
     4561 cgcatctcgc cgtcggcgac ctcgtcgacg ccgacgaggt cgaggcgcct caccgtgggc
     4621 gccgatgtcg tggaggcatc aggcagcaga tgaggtggcc gccgcggcat gcgcggctcc
     4681 ggcgtcccac ttcgcctcga gcagttcgcg gagcgtgttc tgggcggaaa cgagcggcac
     4741 ccgcgccacg accggctcga ggaagccgag gacgatgaac ttgcgcgcct cctcggggtc
     4801 gatgccgcgg ctctcgaggt agaagagctg ggtctcgtcg atcgggccga cggcgctcga
     4861 gtgggccgcc cggcggcagt ccggctgatc gatctcgagc gacgggatcg cgacggcgcg
     4921 ggcctgcttg gacaggttca tcccgaactc gccgaggaag ctgtcagtcc cgaccgcggt
     4981 cggctcgatg atgatcaggc ccttcatgta cgaccgcgtc gcatccatca gggctgcctt
     5041 cgagagcagg ttgcccgtcg tgtcgcggcc gatgtggcgg gtgtacgagg tcaggtcgaa
     5101 cagctggtcg tcggcgccga agacgatctc gacctgctcg accgagctcc ggttgccgct
     5161 gaggcgattg tccacgcgcg accggacgag ccggccaccg agctgtgcca gcgcccagtg
     5221 gagtgacgcg ccctcgccga tctcggcctg gcgatgctgg aacgcgaccg tgcccgtcgc
     5281 gaggttctgg atcgaggcga tcgcgagctc ggcgccggcg gcgagatgga cctcgagcga
     5341 gccggtgaac agcgcctggg ctgcggtccg atcgtgcgcg ccggacgatg cctcgagctc
     5401 ttccacgatc gatgccgacg cgcccttgcc gagatcgagg atcgtccggg tgagcatcgc
     5461 ccgaccgggc tcgcccgcct gccagcggac cacgatcggc cgctcgagcc ggacaccggc
     5521 cggcacgtgg acgacgacgc cctggttcca gcccgcccgg gtgacctggg cgagccgatc
     5581 gtcgagcggc agtgaggtct cgtcgccgag gagccggcgg gccagggctt cgtcgcgcgc
     5641 gatcagcccg gcgaggctgt cgaggacgac tccggccgca cgtgtggcct cgtcgagaag
     5701 gagggcttcg acccggtcct cggtgaacgc cgcgaaccct gcggcgccgt cggggagggg
     5761 ctgggatgca ccgccgacgg gtgccccggc cggcgactcc cacggcgtca cagcggtgag
     5821 atcggcggtt cgcaggtcga cgtaggtcgt gtagagctgg ttggtctcga tcggcagggc
     5881 ccggtagcgc gcgaacgcgg cgctgcgatc ggccgtcagc cagtcgggct cggcgacggc
     5941 gtgggccgcg accgtcgcct cctcgagggt gaacgtcaac tcgttcgggg agcgccgcgg
     6001 agcgcgagcg ggtcgggccg gcgccgccgg ggtcgccggg gtcgcctcgg gtgtggcgtc
     6061 gatcacccga gcgacccctc catctcgagc ttcacgagcc ggttgaactc gatcgcgtat
     6121 tccatcggga gctccttggt gaagacctcg aggaagccct ggacgatcag gttctgcgcc
     6181 tcgttctcgg tcaggccgcg gctcatcagg tagaagacct gctccgcgct gaccttgccg
     6241 acggtggcct cgtgggtcat cgtcgtgtcg tcttcctgga tgtcgatgta ggggtacgtg
     6301 tcgctccggc tgatgtc
//
LOCUS       DATAIW010000088        13101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_180661, whole genome shotgun sequence.
ACCESSION   DATAIW010000088 DATAIW010000000
VERSION     DATAIW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180661"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VHM48_02955"
     CDS             <1..1475
                     /locus_tag="VHM48_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2754392.1"
                     /translation="AGFLGAPSLLAAIGGFVIDHQPPGAKDLVVGLFGTNDKLALEGV
                     IVIVAAVIGAGLGVVAVRRSYAIAAAGFAAFALAGFLATLPMPSATPTTAMIVALVAG
                     IAGVQVMSFLLRTAGAGRTATGAPVAAVMPDWSRRGFLVQAGAVAVASTAAGWFGRRL
                     LEGGVTTVGGTPATTLPGPVSVVTIPAGAELQQDGLTPLVMPNESFYRIDTALITPSV
                     DVASWKLRVHGLVDHEISLSYADLAELPLFEQYVTISCVSNEVGGKLVGNAKWTGVKL
                     RDVLAMAGVQAGATQLVGRSVDGWTAGMPTAWVMDPAREPMIALKMNDVPLPRNHGFP
                     ARLIVPGLYGYVSATKWLSDLELTTLEAFNGYWVPLGWAKEAPILTQSRIDTPSSGQG
                     VAATRVPIAGIAWAPDRGISKVEVAIDGVWQDARLSTPISNATWVQWLVAWDAAASGP
                     GSHTIEVRATDGSGIVQTTDVTPPAPDGARGHHTIQVTVG"
     gene            1697..2368
                     /locus_tag="VHM48_02960"
     CDS             1697..2368
                     /locus_tag="VHM48_02960"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX2754393.1"
                     /translation="MPRRLFLFDPPDRFVAGTIGQPGNRTFFLQARRASQIVSVVLEK
                     VQVAVLAERLGVLLDELEARGIAQPAEVVMADSSPLDEPINETFRATTLTLGWDGDTE
                     QILVEARAETDEDEEDEEDDEEETDDPGDVDDDAEVIDLSAVEGLAGSPAGELLAAFE
                     GIDEDELDGPDTLRVRLTVEVARAFVNRAVEVVAAGRLPCPLCGQPLDPQGHICPRRN
                     GHYVN"
     gene            2391..3122
                     /locus_tag="VHM48_02965"
     CDS             2391..3122
                     /locus_tag="VHM48_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX2754394.1"
                     /translation="MIDTATSLAVLADGEIEIVGRIMGSSNHAMLCRVRLPCPPPEAS
                     TTVEAVYKPTAGERPLDDFPDETLSHREVAAFLVSEAIGWGIVPPTIRRDGPFGDGSL
                     QLWIDIDPTVDVVAMVVEDDPRLRRIAVFDAAVNNTDRKGGHLLPTSDGHIFGVDHGV
                     TFSTVPKLRTVLWGWRGRPFAADELEGLTRLADALRGDVARDLAGLLSRGEIAAMRRR
                     VDALLETRRFPLPRPDWPAIPWPPF"
     gene            complement(3138..3413)
                     /locus_tag="VHM48_02970"
     CDS             complement(3138..3413)
                     /locus_tag="VHM48_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754395.1"
                     /translation="MRGDRVEVVVDTGQGVQTFDIVATKNGRRLEITTARGVVEVSEL
                     TRGGTPVRTGRFMASRLIALVEHPAQEQPDSRVEVQTRRRIRPPEAG"
     gene            3641..5263
                     /locus_tag="VHM48_02975"
     CDS             3641..5263
                     /locus_tag="VHM48_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2754396.1"
                     /translation="MRRWNGWIEEGSQPPHVAAEAKRLLEAEIGPGRPTADARLEDVI
                     AALPAGRLADDDGGRLSLDPADRIRHARGQSLPDWIATRSGRLGRVPDAVARPADATA
                     VRELFALARTRGAVLIPYGGGTSVVGGVSPDRDDDRPAITVALDALAGLRELDERSGL
                     ATFGAGTLGPDLEAALAPHGLTLGHFPQSWEGSTVGGWVAARSAGQQSIGFGRIEALL
                     AGGHVEAPAGTLDLPSHPASAAGPDLRQLVLGSEGRFGILTDAILRVVPKPPVEAVPS
                     WFLPDWPRALEAAHEIGRAGLPLSMVRLSTPLETATFLALAGGGRRVRALRAYLGFRR
                     IGPEPCLLLVAASGREKFVELAVREVGSIVRRHGGTGVGGSLGREWLRSRFRTPDLRD
                     SLWDLGYAIDTLETAVDWRRLPDLAAAVGRALRHGLEAGGTGERVHAFSHLSHVYPTG
                     SSLYATYVFRIAADPDETLDRWRQLKTAASDVIVEHGGTISHQHGVGRDHRPWLAAEK
                     GELGLAVLGDVARRLDPDRLLNPGVLLGEEGQ"
     gene            5260..6819
                     /locus_tag="VHM48_02980"
     CDS             5260..6819
                     /locus_tag="VHM48_02980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2754397.1"
                     /translation="MSADQVLAVDVGTQSVRALVFDPGGSLVAHARIPIEPYVSPQPG
                     WAEQDAELYWRSIGEACAALWAGGVARRDAIAGVALTTQRGTIVVTDEAGVPLRPAMV
                     WLDQRRAEGVAPIGGLNGLAFRALGVRRTVAAFQADCEANWLRTNEPAVWRRIRRYLL
                     LSGFLAHRLTGRFVDSVGSQVGYLPFDYKRLAWAKSGDWRWTAAPIDPAWLPELVEPG
                     ARLGELTAAAAAVVGLPAGTPVIAAAADKACEVLGSGALEPHIGSLSYGTTATINTTQ
                     RRYVESVPLVPPYPAAVPGAYSLEVQVYRGYWLVDWFKRQFGAAEVAQADALGIAAEA
                     LFDDLVRATEPGSMGLMLQPTWTPGVRIPGPEAKGAMIGFGDVHTRAHLYRAILEGLA
                     YALREGGERAAGRAKVPLRELRVSGGGAQSPAAIQLTADIFGLPTGVPHTYETSGLGA
                     AIDAAVGLGLHPSIDAAVGAMTRLTRTIEPDPERQRLYDQLYRRVYRKMYDRLKPLYE
                     DIMQITGYPPS"
     gene            complement(6797..7087)
                     /locus_tag="VHM48_02985"
     CDS             complement(6797..7087)
                     /locus_tag="VHM48_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754398.1"
                     /translation="MVDGPGQSVTEALANAGVGPLLVNGILLKEADDTVWLSEALLRS
                     SPPQCAEPKPLVENWSTEDQTFVNGEGIHVADGVRWVERAQLVGVVSSAGSR"
     gene            complement(7585..8076)
                     /locus_tag="VHM48_02990"
     CDS             complement(7585..8076)
                     /locus_tag="VHM48_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754399.1"
                     /translation="MPRLADNRRLIKAADWTASHRLAGPFAIGLASIAALLAPFPLAI
                     GLRALADSVDDSILTAVIFIPTLLIVLLPYPIFFGGWLPGYVVGRSNVGRTGWWLLAA
                     GFLAPLAIPLVVMMFSMLRSYGGSALTLPLLVPLLLIPLRLGYRRGCRTLSPSADGLI
                     SPP"
     gene            complement(8235..8966)
                     /locus_tag="VHM48_02995"
     CDS             complement(8235..8966)
                     /locus_tag="VHM48_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2754400.1"
                     /translation="MRDERSRADAPDADRRGRFDERSPACAPGCACACAVGNEFGERQ
                     ARRDLALYRRRGPDRTTGWLIEGLTGGASDGVDGLSVLDIGAGVGAVHLELLRAGASS
                     ATDVDGSPAYQAVARGEAARQGVGDRVAYALGDFVEVARTIEPADLVALDRVVCCYPD
                     MEALVRLSVGRARRRYGLVYPRDTRWIRAASRMLNMVSRLLRRRFRTWVHRTADVDAI
                     ARAAGFAPQFERTTLFWQVVVYERP"
     gene            complement(9240..10349)
                     /locus_tag="VHM48_03000"
     CDS             complement(9240..10349)
                     /locus_tag="VHM48_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2754401.1"
                     /translation="MTADSTPLPSAPGVADPLPDARAPEVVRRPRRDDGAIPSRRERS
                     VPHAGWMVVAGKEFADHLLSARFFVLLAILGMAGGIPIFLATEQIRNLASQLPPGISG
                     LFVGLFIIGPSDVSIFNIDVTVQSFITLAAPLLGLAFAFDAINGERAQGTLPRLLSQP
                     IHRDDVINGKFAAGLAIIAFVLAVVVGVICAFGILRLGIAPTASEVLRLIVWFAVTVL
                     YVGLWLSFGLLLSILFRRASTSALVGFGVWLVVAVFGQFLVRFLLAFLVPTTNALTAD
                     NLSAIQTNTFILRLLPTTLYNEISRVLLNPTATYAATPPTIGQNVQVSQQIGSVLSID
                     QSFIIVWPQIATLLAIMVVMFAIAYVRFMRQEVRA"
     gene            complement(10346..11452)
                     /locus_tag="VHM48_03005"
     CDS             complement(10346..11452)
                     /locus_tag="VHM48_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754402.1"
                     /translation="MTAPSTRPTRKKVGRQRVGSDADPKATTSRPRVEGDDRPRAPIR
                     TRGLTKAYGDLIAVDHLDLEVQAGEIFGLLGQNGAGKTTTILMLLGLTEPTEGQARVV
                     GLDPARRPLEVKRRVGYLPDAVGFYADLSGRDNLRYTARLNRIPRAEAEAAIDEALEQ
                     VGLAHRADDQTEQYSRGMLQRLGIADALIKDPDVLILDEPTTAIDPLGVTEILDLLRS
                     LVEERQMAILLSSHLLNQVQSVCDRIGIFAAGRLIGQGTMGDLAHRFGEDIAHVEAEV
                     QTNDDAEVARVREVLGAIPSVSGLTAPRRGNPAWSIEVKPAADETRVRRSILAAAAST
                     GLDLTSVRSVAPSLEEIYRRAVERAAHGHARDVR"
     gene            complement(11449..12636)
                     /locus_tag="VHM48_03010"
     CDS             complement(11449..12636)
                     /locus_tag="VHM48_03010"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX2754403.1"
                     /translation="MPTSLIARARRPVIALAAAAVLMVGLVPAVTAADSLAVTTPYPS
                     ISVAPGSKASFDLTVTAPESGTVALAVSGAPTGWKATLHGGGFVVNGVTAAPGKPGAA
                     RLDVDVPADATATKATLTVDARQGSQHTSLTITIGVDAAVAGDITLTTSSGTLTGSSD
                     SPFTFDLTLQNGSAEDQTVSATATGPAGWTISTKLSQANAASILVKAGSSTSITVTAT
                     PPVGAPAGHTDLNVTATAGTKTITGKLGVDITGSFTMTMSTPNDLLSAHGSAGSESKV
                     TLEIQNTGTATLDGVAVTASPPTGWTVTFDPKDPISVPAGTAPGTITATIVPSADAVA
                     GDYVVSFNASSAAAGTSAAAKASADIRFTVETSPLWALVGIGVIVAIVLALFYVFRTY
                     GRR"
     gene            12820..>13101
                     /locus_tag="VHM48_03015"
     CDS             12820..>13101
                     /locus_tag="VHM48_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2754404.1"
                     /translation="MTAFDIERARAETPGVVNVIHLNNAGSALMPAVVVERMVEYLGR
                     EAAVGGYEAEAEASTDVAGVYGSIATLIGAAPDEIAICENATRAWDMAFY"
BASE COUNT         1952 a         4630 c         4680 g         1839 t
ORIGIN      
        1 tcgccgggtt cctcggggcg ccgtcgctgc tcgccgcgat cggtggcttc gtgatcgacc
       61 accagccacc cggcgccaag gacctcgtcg tcgggctgtt cggaaccaac gacaagctcg
      121 cgctcgaagg cgtcatcgtc attgtcgcgg cggtcatcgg ggcggggctc ggggtcgtcg
      181 ccgtgcgccg gtcgtacgcg atcgcggccg ccggcttcgc cgcattcgcc ctggcgggct
      241 tcctcgccac gctcccgatg ccgtccgcga ctccgaccac cgcgatgatc gtcgccctcg
      301 tcgcgggcat cgccggggtc caggtgatgt cgttcctcct gcggacggcc ggtgccggtc
      361 ggaccgcgac cggcgcgccg gtggccgcgg tcatgcccga ctggtcgcgg cgcggcttcc
      421 tcgtccaggc cggggcggtc gccgtcgcgt cgaccgccgc cggctggttc ggccggcgac
      481 ttctcgaggg cggcgtgacg accgtcggcg ggacgcccgc cacgaccctg ccgggtcccg
      541 tctcggtcgt cacgatcccg gccggggccg agctccaaca ggacggcctc acgccgctcg
      601 tcatgccgaa cgagagcttc taccggatcg acacggcgct gatcaccccg tcggtcgacg
      661 tcgcgagctg gaagctccgc gttcacgggc tggtcgacca cgagatcagc ctgtcctacg
      721 cggacctcgc cgagctgccc ctgttcgagc agtacgtcac gatctcgtgt gtcagcaacg
      781 aggtcggcgg gaagctcgtc ggcaacgcca agtggacggg cgtgaagctc cgcgacgtcc
      841 tcgcgatggc cggcgtccag gcaggagcca cgcagctcgt cgggcgatcg gtcgacggct
      901 ggaccgcggg catgccgacg gcctgggtca tggacccggc acgcgagccg atgatcgcgt
      961 tgaagatgaa cgacgtcccg ctgccgcgga accacggctt tcccgctcgc ctgatcgtgc
     1021 ccgggctcta cggctacgtg tcggcgacga aatggctgag cgacctggag ctgaccaccc
     1081 tggaggcctt caacggctac tgggtccccc tcggctgggc caaggaggcc ccgatcctga
     1141 cgcagtcgcg gatcgacacg ccgtccagcg gccagggcgt cgcggcgacc cgcgtgccga
     1201 tcgccgggat cgcgtgggcc ccggaccgtg gcatctcgaa ggtcgaggtc gcgatcgacg
     1261 gcgtgtggca ggacgccagg ctctcgacgc cgatctcgaa cgcgacctgg gtccagtggc
     1321 tcgtcgcctg ggatgcggct gcgtcgggac cgggcagcca cacgatcgag gtccgggcaa
     1381 ccgatggcag cggcatcgtc cagacgaccg acgtcacgcc gccggcgcct gacggtgccc
     1441 gcggccatca cacgatccag gtgaccgtcg ggtagccgcc tgcccggatt ccgacatttc
     1501 catcgggcgt agtgagagtc cgacgccgcg gtcggaatcc cggtcagcca ccatccccga
     1561 tggtgcccga ccgggaatca gtccgattct gctccggagt acgtcgaatc cctgacgaca
     1621 accaccccag atggtggacg gcaggactct ctgaacgatg tcggcgcgtg tctcgatctc
     1681 aggctacagt ccgctgatgc ctcgacgact cttcctcttc gacccacccg atcgcttcgt
     1741 ggccggcacg atcggccagc ccggcaaccg gacgttcttc ctccaggccc ggcgggcgag
     1801 ccagatcgtc tcggtcgtcc tggaaaaggt ccaggtggcc gtcctcgccg agcggctggg
     1861 cgtcctcctc gacgagctcg aggcccgcgg gatcgcccag ccggccgagg tcgtcatggc
     1921 cgactcgtcc ccgctcgacg agccgatcaa cgagaccttc cgggcgacga ccctcaccct
     1981 cggctgggac ggcgacacgg agcagatcct cgtcgaggcg cgggccgaaa ccgacgagga
     2041 cgaagaggac gaagaggacg acgaggagga gaccgacgat ccgggcgatg tcgacgacga
     2101 cgccgaggtc atcgacctgt ccgcggtcga aggcctggcc ggctcgcccg caggcgagct
     2161 cctcgccgcg ttcgagggga tcgacgagga cgagctcgac ggacccgaca cgctccgggt
     2221 ccggctcacc gtcgaggtcg cccgggcctt cgtgaaccgg gcggtcgagg tcgtcgccgc
     2281 gggccgcctg ccctgtccgc tgtgtggcca gccgctcgat ccccagggcc acatctgccc
     2341 gcgtcgcaac ggccactacg tcaactgatc ggccgggcgc gagcaccccg atgatcgaca
     2401 cggccacctc gctcgcggtc ctggccgacg gcgagatcga gatcgtcggg cggatcatgg
     2461 gctcgtcgaa ccacgcgatg ctctgccgcg tccggctgcc gtgcccaccg cccgaagcgt
     2521 cgacgacggt cgaggcggtc tacaagccga ccgccggcga gcgcccgctc gatgacttcc
     2581 cggacgaaac gctctcccat cgcgaagtcg cggcctttct cgtcagcgag gcgatcggct
     2641 gggggatcgt gccgccgacg atccggcggg acgggccgtt cggcgacggc tcgctgcagc
     2701 tctggatcga catcgacccg acggtcgacg tcgtcgccat ggtcgtcgag gacgacccgc
     2761 ggctgcgccg gatcgcggtc ttcgacgcgg ccgtgaacaa caccgatcgg aagggcggcc
     2821 acctcctgcc gacgagcgac ggccacatct tcggcgtcga ccacggggtg accttctcga
     2881 ccgtaccgaa gctccggacc gtcctctggg gatggcgcgg ccggcccttc gcggccgacg
     2941 agctcgaggg cctgacccgg ctcgccgacg cgcttcgcgg cgacgtcgcc cgcgatctgg
     3001 cagggctgct gtcgcggggc gagatcgcgg ccatgcggcg gcgggtcgac gcgctcctcg
     3061 agacgcggcg ctttcccctc ccccgccccg actggccggc gatcccgtgg ccaccgttct
     3121 gagcgacggc cttgaggtca gccagcttcc ggcggccgga tgcgccgccg cgtctgaacc
     3181 tcgacccggc tgtcgggctg ctcctgggcg ggatgctcga caagcgcgat gagccggctc
     3241 gccatgaacc ggcctgtccg gacgggcgtc ccgccgcggg tcagttcgct gacctcgacc
     3301 acgccgcgag cggtcgtgat ctcgagccgg cggccgttct tcgtcgccac gatgtcgaac
     3361 gtctggacgc cctggcccgt gtccaccacg acctcgaccc gatcgcctcg catggcggca
     3421 cggtagcact gccggcgtca cgggcgcggg gtggccatgg ccgcgaggtt caacccggcc
     3481 gcagccccgt cgtgaggatt gactgccacg gcatgctccc gaaccccgat cgcgccggga
     3541 acggtaggct gcccttgaac cgattcggag atctccgagg tccagggcgg aggtctccga
     3601 cgccattggc ccgagcaacc gttggcccga ggaggtgggc gtgcgacgct ggaacggctg
     3661 gatcgaagaa ggttcccagc cgccgcacgt cgcggcggag gcgaagcggc tgctcgaggc
     3721 cgagatcggg cccggtcggc cgactgcgga cgcgaggctg gaggacgtca tcgccgcgct
     3781 tccggccggc cgactggcgg acgacgacgg cggtcggctc tccctcgatc ccgccgaccg
     3841 gatccgtcac gcccgcggcc agagcctgcc cgactggatc gccacgcgga gcggtcggct
     3901 cgggcgcgtg cccgacgccg ttgcccggcc tgccgacgcc acggccgttc gggagctgtt
     3961 cgcccttgcc cgcacgcgcg gcgccgtcct gatcccgtac ggcggtggga cgtccgtcgt
     4021 tggcggcgtc agcccggatc gcgacgacga tcgacccgcg atcaccgtcg cgctggacgc
     4081 cctggccggc ctgcgcgagc tcgacgaacg gagcggcctc gcgaccttcg gcgcgggcac
     4141 gctcgggccg gacctcgagg cggcgctcgc gccgcacggg ctgacgctgg gccacttccc
     4201 ccagtcgtgg gaaggctcga cagtcggcgg ctgggtcgcg gcgcgctcgg ccggccagca
     4261 gtcgatcggg ttcgggcgga tcgaggcgct cctcgccgga ggccacgtcg aggcccctgc
     4321 cggaaccctg gacctcccgt cgcacccggc gtcggcggcc ggtccggacc tccgccagct
     4381 cgtcctcggc tcggagggcc ggttcgggat cctgaccgat gccatccttc gcgtggtgcc
     4441 caaaccgccg gtcgaggccg tcccgtcatg gttcctgccc gattggccgc gcgcgctcga
     4501 ggccgcccac gagatcggcc gggccgggct gccgttgtcg atggtccggc tctcgacacc
     4561 gctcgagact gccaccttcc tcgccctcgc cggcggcggc cgccgcgtcc gggcgctccg
     4621 ggcgtacctc gggttccgca gaatcgggcc ggagccgtgt ctcctgctcg tcgccgcgag
     4681 cggtcgcgag aagttcgtcg agctcgccgt ccgcgaggtc ggctcgatcg tccggcgtca
     4741 cggcgggacc ggcgtcggcg gttcgctcgg gagggagtgg cttcgcagcc ggttccggac
     4801 gccggacctg cgcgactcgc tgtgggatct cggctacgcg atcgatacgc tcgagaccgc
     4861 ggtcgactgg cggcggctgc cggatctcgc cgcggcggtc ggacgggcgc tccgccacgg
     4921 cctcgaggcc ggcgggaccg gcgagcgggt ccacgccttc agccacctct cccacgtcta
     4981 cccgaccggc tcgagcctct acgccaccta cgtcttccgg atcgccgccg accccgacga
     5041 gaccctcgat cgctggcggc agctgaagac cgcggcgagc gacgtgatcg tcgagcacgg
     5101 cgggacgatc agccaccagc acggcgtcgg gcgcgaccac cggccgtggc tcgccgccga
     5161 gaagggcgag ctcggcctgg cggtcctcgg cgacgtcgcc cggcgtctcg atccggaccg
     5221 cctgctgaat cccggcgtcc tgctcggcga ggaggggcaa tgagcgccga ccaggtcctg
     5281 gccgtcgatg tcggcaccca gagcgtccgg gcgctcgtct tcgatccggg cgggtcgctc
     5341 gtcgcccatg cccggatccc gatcgagccg tacgtctcgc cgcaaccggg ctgggccgag
     5401 caggacgccg agctctactg gcgctcgatc ggcgaggcat gcgcggccct gtgggccggg
     5461 ggcgtcgccc gtcgtgacgc gatcgcgggt gtcgcgctca cgacccagcg cgggacgatc
     5521 gtcgtgaccg acgaggctgg cgtgccgctc cggccggcga tggtctggct cgaccagcgt
     5581 cgggccgagg gggtcgcgcc gatcggcggc ctgaatggcc tcgcgttccg cgccctcggt
     5641 gtccgccgga ccgtggccgc gttccaggcc gactgcgagg cgaactggct cagaacgaac
     5701 gagccggccg tctggcggcg gatccgtcgc tacctgctcc tgtccggctt tctggcccac
     5761 cgcctgacgg gacggttcgt cgactcggtc ggaagccagg tcggctacct cccgttcgac
     5821 tacaagcgcc tcgcctgggc gaagagcggc gactggcgct ggacagcggc gccgatcgac
     5881 ccggcctggc tgccggaatt ggtcgagccg ggcgcgcgcc tcggcgagct caccgccgcc
     5941 gccgcggcgg tcgtcggcct gccggccgga acgcccgtga tcgccgccgc ggccgacaag
     6001 gcctgcgagg tgctgggttc gggcgccctg gagccgcaca tcggatcgct gtcgtacggc
     6061 acgaccgcaa cgatcaacac cacccagcgg cgctacgtcg agtcggtgcc actggtcccg
     6121 ccatatccgg ccgcggtccc cggggcgtac agcctcgagg tccaggtcta ccgcggctac
     6181 tggctcgtcg actggttcaa gcgccagttc ggtgcggccg aggtggccca ggcggacgcc
     6241 ctcgggatcg ccgccgaggc gctgttcgac gacctcgtcc gggcgaccga gcccggctcg
     6301 atgggcctga tgctccagcc gacctggaca ccaggcgtgc ggatcccggg acccgaggcg
     6361 aagggcgcga tgatcggctt cggcgacgtc cacacccggg ctcatctcta ccgggcgatc
     6421 ctcgagggcc tcgcctacgc cctgcgcgag ggcggcgagc gagccgccgg ccgggcgaag
     6481 gtgcccctgc gggagctgcg cgtatcgggc ggcggcgccc agtcgccggc cgccattcag
     6541 ctgacggccg acatcttcgg gctgccgacg ggcgttcccc acacctacga gacgtccggg
     6601 ctcggtgccg cgatcgacgc ggccgtcggg ctgggcctgc atccgtcgat cgacgcggcg
     6661 gtcggcgcga tgacaagact gacccggacg atcgagccgg accccgagcg ccagcgcctc
     6721 tacgaccagc tctaccgacg cgtgtaccgg aagatgtacg accgcctgaa gccgctgtac
     6781 gaggacatca tgcagatcac cggctacccg ccgagctgac gacgccgaca agctgcgccc
     6841 gctccaccca gcggacgccg tccgccacgt gaatgccttc gccattgacg aaggtctggt
     6901 cctcggtgga ccagttctcg acgagcggtt tcggctctgc gcattgcggt ggtgacgacc
     6961 tcagcaaggc ctcgctcagc cacacagtgt catcggcctc cttcagcagg atgccgttca
     7021 ccagcaacgg cccgacgccc gcgttggcca gggcctcggt gacgctttga cccgggccgt
     7081 cgaccatgcc gaaggctccc agcttcagcg tggcggtggc ctgcccactg ggagcgggcg
     7141 aggtccacgt cggactcggc acagcgccag tcacggcgct cggcgtagga cccgccgggc
     7201 cagctccaca ggcggctgtc gcagccgcga tcagggtgat gaccacgatc cgcaggtgac
     7261 ttggacccat ctgaatgctc cccaacgtcc aggacgcgga gtccaacttc gggggaagga
     7321 ctcgactcgc gaacgcccat ttggttgttg ggcagatcca cggctattgg tcaggacgcg
     7381 actgcaatgg gtctgttacc ggtcgtcggc ggccccgcga tccccttcgg aggatgacgt
     7441 cgactcacgg gcgttcccca tacccacgag acgtccgagc tcggcgcggc gatcgacgcg
     7501 gcggtcgggc tggggctcga gctgatcacc ggccccggcg gcgccatccc gtcccggacc
     7561 accgggacgg acgaacggcc ttcgtcaggg cggcgagatc aatccgtcgg ccgacggcga
     7621 cagcgtgcga caaccacgtc gataccccag acgcagcggg atcaacagca gcggaacgag
     7681 gagcggcagg gtcagcgcgc tcccgccata cgacctcagc atcgagaaca tcatcacgac
     7741 gagcgggatc gcgagtggag cgaggaatcc cgcggcaagc agccaccaac ccgtccgccc
     7801 gacgttcgac cgcccgacga cgtacccggg cagccagccg ccgaagaaga tcgggtacgg
     7861 cagaagaacg atcaggagcg tcgggatgaa gatgacagcc gtgagaatcg agtcgtccac
     7921 gctgtcggcc agcgcacgga ggccgatcgc aaggggaaac ggagccagaa gggcagcgat
     7981 cgacgccaat ccgatggcga atggccccgc gagccgatga ctggccgtcc agtcagccgc
     8041 cttgatcagt cgtcggttgt cagcgaggcg cggcacggtg agctccggtc catcttgtcg
     8101 agagtcgccg acagccgctc ggcgggtgga aggccgcgga ctggaccttg cggcgcttgc
     8161 tggaaggacg ccggaaccgc gtcgacgtga cggcaggcct accgccgatc acagcgagcg
     8221 ggggcttgcc gggctcaggg ccgctcgtag acgacgacct gccagaagag ggtcgtccgc
     8281 tcgaattgtg gtgcgaaccc ggccgcccgg gcgatcgcgt cgacgtcggc ggtccggtgg
     8341 acccatgtcc ggaaccgtcg gcgcagcagg cgggacacca tgttcagcat ccggctcgct
     8401 gcccgaatcc agcgcgtgtc gcgcggatag acgagcccgt accgtcgccg ggcgcggccg
     8461 accgacaggc gcaccagcgc ctccatgtcc gggtagcagc agacgacgcg atcgagggcg
     8521 acgaggtcgg ccggctcgat cgtccgcgcc acctcgacga agtcgccgag cgcgtacgcg
     8581 acccgatcgc cgacgccctg gcgggcggcc tcgccgcgag cgacggcctg gtacgccggc
     8641 gagccgtcga cgtcggtagc gctcgacgcg ccggctcgca gcaattcgag atggaccgcc
     8701 ccgaccccgg ccccgatgtc gaggacggac aggccatcaa cgccgtccga cgccccgccg
     8761 gtcaggccct cgatgagcca gcccgtggtc cggtcgggcc cgcgccggcg gtagagcgcc
     8821 agatcccggc gcgcctggcg ctcaccgaac tcattgccga ccgcgcaggc gcaggcacag
     8881 cccggagcgc aggccggcga gcgctcatcg aaccgcccac gccgatccgc gtccggtgcg
     8941 tcagcccgac tgcgttcgtc gcgcatgagt gcgtcagctc atggcggccg cgagcaccgc
     9001 agcgagcggc ctcggatggc cgtgagcgag gagcgcagcg gctaacgccg cgatggcgtg
     9061 ctcagccgcg acagccggaa cagcgacggc cggaacagcg ttcgttgcgg atgggcgagg
     9121 ctggcaaggc gccgccgagg accgcagcga gcgacctcgg atggtcgtga gcgaggcccg
     9181 caggcggcaa cgccgccagc gtcgtccagc cgcgacagcc ggaagtcttg ccacgattcc
     9241 taggcgcgga cttcctgtcg catgaagcgg acatacgcga tcgcgaacat gacgaccatg
     9301 atcgcgagca gcgtcgcgat ctgcggccag acgatgatga agctctggtc gatcgacaga
     9361 accgacccga tctgctgcga gacctggacg ttctggccga tcgtgggtgg cgtcgccgcg
     9421 tatgtcgcgg tggggttcag cagcacccgc gagatctcgt tgtaaagggt cgtcggcaac
     9481 aggcgcagga tgaacgtgtt cgtctggatg gcgctcaggt tgtcggcggt cagcgcgttc
     9541 gtggtcggga cgaggaaggc gagcaggaac cggaccagga actggccgaa gacagcgacg
     9601 acgagccaca ccccgaagcc gacgagggcc gaggtggacg cgcgccggaa gaggatcgac
     9661 agcaacaggc cgaacgacag ccacagtccg acgtacagca cggtcaccgc gaaccagacg
     9721 atcagacgca gcacctccga cgcggtcggc gcgatcccca accggaggat gccgaacgcg
     9781 cagatgaccc cgacgacgac cgcgagcacg aacgcgatga tcgcgagccc ggcggcgaac
     9841 ttgccgttga tcacgtcgtc gcgatggatg ggctgcgaca ggagccgcgg caacgtcccc
     9901 tgggcacgtt cgccgttgat cgcgtcgaac gcgaacgcca gcccgagcaa cggcgcagca
     9961 agggtgatga acgactggac ggtgacgtcg atgttgaaga tcgagacgtc gctcgggccg
    10021 atgatgaaca ggccgacgaa cagccccgag atgcccggcg gcagctgcga ggccaggttc
    10081 cggatctgct cggtggcgag aaagatcggg atcccgccgg ccatgcccag gatggcgagc
    10141 aggacgaaga accgcgcgga caggagatgg tccgcgaact ccttgccggc caccaccatc
    10201 cagccggcgt gcgggacgga ccgttcgcgg cgcgacggga tcgccccgtc atcgcggcgc
    10261 gggcggcgga cgacctccgg cgcgcgggcg tcgggcaagg gatcggcgac tccgggcgcg
    10321 gacgggagcg gcgtgctgtc ggcggtcatc ggacgtccct cgcgtggccg tgggcagccc
    10381 gttcgaccgc gcgccggtag atctcttcga gcgatggagc gacggaccga accgaggtca
    10441 ggtcgagacc ggtcgacgcg gcggcggcaa ggatcgagcg ccggacgcgc gtctcgtcgg
    10501 cggccggctt cacctcgatc gaccacgccg ggttgccgcg ccgcggggcc gtcaggccgc
    10561 tgaccgacgg gatcgcgccg aggacctcgc gaacgcgcgc gacctcggcg tcatcattcg
    10621 tctggacctc ggcctcgaca tgggcgatgt cctcgccgaa gcggtgggcg aggtcgccca
    10681 tcgtcccctg cccgatcaac cggcctgcgg cgaagatccc gatccgatcg cagaccgact
    10741 ggacctggtt cagcagatgg ctcgacagga ggatcgccat ctggcgctcc tcgacgagcg
    10801 agcggaggag gtcgaggatc tcggtcacgc cgagcgggtc gatcgcggtt gtcggctcgt
    10861 cgaggatcag aacatccgga tccttgatca gcgcgtcggc gatgccgagc cgctggagca
    10921 tgccccggga gtattgctcg gtctggtcgt cggcacgatg ggccagcccg acctgctcga
    10981 gggcctcgtc gatcgccgcc tctgcctcgg cgcgcgggat ccggttgagg cgagcggtgt
    11041 agcggaggtt gtcacggccg ctcaggtcgg catagaaccc gaccgcgtcg ggcaggtagc
    11101 cgactcgccg cttgacctcg agcggcctgc gggccgggtc cagcccgacg acgcgcgcct
    11161 ggccctccgt cggctcggtc agcccgagga gcatcaggat cgtcgtcgtc ttgccggccc
    11221 cgttctggcc gagcaggccg aagatctcgc cggcctggac ctcgagatcg aggtggtcga
    11281 cggcgatcag gtcaccgtag gccttggtca ggccgcgcgt ccggatcggg gcacgcggcc
    11341 gatcgtcacc ctcgacccgc ggccggctgg tcgtcgcctt cgggtcggcg tccgacccga
    11401 cgcgctgccg gccgaccttc ttgcgggtcg gccgggtcga cggggccgtc atcgacgccc
    11461 gtaggtccgg aagacgtaga acagcgcgag gacgatcgcg acgatcacgc cgatcccgac
    11521 cagggcccac aggggcgagg tctcgaccgt gaaccggatg tcggcgctgg ccttcgccgc
    11581 ggcgctcgtt cccgccgccg cgctcgacgc gttgaacgac acgacatagt cgccggccac
    11641 ggcatccgcc gaggggacga tcgtcgcggt gatggtgccc ggcgcagtac cggcagggac
    11701 cgagatcggg tccttcgggt cgaacgtgac cgtccagccg gtcggcggcg acgcggtgac
    11761 cgccaccccg tcgagcgtcg cggtcccggt gttctggatc tcgagcgtca cctttgactc
    11821 ggatccggca gacccgtgag ccgacagcag atcgttcggg gttgacatcg tcatggtgaa
    11881 cgagccggtg atgtcgacgc cgagcttgcc ggtgatcgtc ttcgtccccg ccgtggcggt
    11941 cacgttgagg tcggtgtgac cggccggcgc gccgaccggt ggggtcgccg tgaccgtgat
    12001 cgacgtcgag ctaccggcct tgacgagaat gctcgcggca ttcgcctggg acagcttcgt
    12061 cgagatcgtc cagccggccg ggccggtggc ggtcgccgag acggtctggt cctcggccga
    12121 cccgttctgg agggtcagat cgaaggtgaa cggcgagtcg ctcgagccgg tcagggtccc
    12181 tgacgatgtc gtgagcgtga tgtcaccggc gacggcggca tcgacgccga tcgtgatcgt
    12241 caggctggtg tgctgagagc cctgccgcgc gtcgacggtc aacgtcgcct tcgtcgcggt
    12301 cgcgtccgcc gggacatcga cgtcgagccg ggcggctccg ggcttgccgg gcgcggcggt
    12361 aacgccgttg acgacgaagc cgccgccgtg aagcgtcgcc ttccaaccgg tcggcgctcc
    12421 ggagacggcc agggcgaccg tgccggactc gggagccgtg acggtcaggt cgaagctcgc
    12481 cttcgagccg ggcgcgacgg agatcgacgg gtacggtgtc gtcaccgcga gtgaatcggc
    12541 ggccgtcacg gcgggcacga gccccaccat gaggacggct gcagcagcaa gggcgatcac
    12601 gggccgtcgg gcacgcgcga tcagggacgt cggcaaggga cccctccgtc agtcggtttg
    12661 catcggaccg acggagtctg ccaactgaat gcggttcacg caacttcggt tcgattcgac
    12721 gtaggttgac atttcgcaac cgaaggcgtc caggagcgcg gcgttggcaa ccggggggcc
    12781 cgcgaagagc gaccatgaac gactcaggag gacatcgctg tgaccgcgtt cgatatcgag
    12841 cgggcccgcg ccgagacacc gggtgtcgtg aacgtgatcc acctgaacaa cgccggctcg
    12901 gccctgatgc cggcggtcgt cgtcgagcgg atggtcgagt acctcggtcg ggaagctgcc
    12961 gtgggcggct acgaggccga ggcggaggca tcgacggacg tcgcgggcgt ctacggctcg
    13021 atcgcaaccc tcatcggcgc cgcccccgac gagatcgcca tctgcgagaa cgcgacccgg
    13081 gcctgggaca tggcgttcta c
//
LOCUS       DATAIW010000089         6165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_33986, whole genome shotgun sequence.
ACCESSION   DATAIW010000089 DATAIW010000000
VERSION     DATAIW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33986"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHM48_03020"
     CDS             complement(<1..429)
                     /locus_tag="VHM48_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2754405.1"
                     /translation="MDDRRTEPNPSIQARLARAFDPAELRRIKRLWVRHSIAEDRRDI
                     EGLVATLAPACVYEMIPTGQRWAGHEGARAFYAELFAAFPDNRFALTEIVVGPQGVFE
                     VATLTGTNEGPWGGAPASGLPVSLQVLILFPWDPVTQRFLG"
     gene            complement(430..1281)
                     /locus_tag="VHM48_03025"
     CDS             complement(430..1281)
                     /locus_tag="VHM48_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX2754406.1"
                     /translation="MTDIWLCGNCHSINRQRTRRCYKCGAEQATADTGEMAMHRQEQA
                     IASRHVVPYRPAAALGMAASIFLLGLAAVSVAVALKSVEIMSFTMTQLDVLKATGRID
                     EAAFNAVATSAGSLGLVRLAIILPTLLFFAAWLSRVVSNVPALGGGLPGSSPWRAFVT
                     TLIPVLNLRTVPGTIQDVLYRLDPKAGGFFMVGLAWVGLVGSWIVSAIASRYLALRVV
                     FDIRNATSLGDAVDAVRGSLTAAFVIDIATAILIAIGSIVLILVMIRIERRSRARDAE
                     VRLAAGV"
     gene            complement(1302..2669)
                     /locus_tag="VHM48_03030"
     CDS             complement(1302..2669)
                     /locus_tag="VHM48_03030"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2754407.1"
                     /translation="MTHGARSAPTPFDTAAIAARAVALADAAGATEAEAVVSAEQAQL
                     TRFANSEIHQNVAETNGTLNLRVAFGRRIGVASSNRLDEDGLRRLAETAVAIARNSAE
                     LEDWGGLPEPTPIDELPAGWSEATAAATPEQRAAGVRAVIAAADAAGVRAFGSFSTAA
                     EHLAVANSHGIRAAQRRTSAELLTVTMGADGGSGYAEQASVDVTAIDAAAIGREAAEK
                     ARATVNPVAVEPGDWSVVLEEYAVVDLLSLLAFMGFSALAVQEERSFAEPGKVIGSEL
                     VTIVDDATEPGAMPMAFDYEGVAKQRVVLVERGVCRDVVYDTQTGARGGHASTGHGLP
                     APNSYGPFPLNMVMSAGTTERAALIGEMERGLLVTRFHYTNPVHPKLAIVTGMTRDGT
                     FLVEGGKIVGPVRNLRFTQSYLAALAGTVAVGRERRTVRGDFGGVLVPAVRIDGWTFT
                     GATEH"
     gene            complement(2677..4128)
                     /locus_tag="VHM48_03035"
     CDS             complement(2677..4128)
                     /locus_tag="VHM48_03035"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2754408.1"
                     /translation="MRDVSDRSLDTAVQLGASYADVRVVHRRDESIAVKSGRVEGVAA
                     GESEGFGVRVLVDGAWGFASSSRLTADEADRVAADAVRIAQASATALRDRVRLDDRPP
                     AHGTYETPIEEDPFAVPLEEKIGHLLAADEAARAVDGVAFVETRYDAVRETRTFGATD
                     GSWTEQTFTHVGANIEANAIEGDDHQRRTYPDADGGPFRAAGYDYVRGLDLVGHAGPL
                     AEEAVALLSAPQCPSGRFTIILDPSQLYMQVHESCGHPTELDRVFGTEASYAGTSFLT
                     TDKLEAGFRYGSDLVDIVADATAPGGLGTFGWDDEGVAAQQVPLVENGIFVGYLSSRE
                     TAPRIGRQSGGAMRADGWNRIPLIRMTNVNLLPKPGLSLDDIVAATDDGLYLASNRSW
                     SIDDRRLNFQFATELAWEIKGGKKGRLFKNPTYTGITYEFWRSLDAVGDESSYVMLGT
                     PNCGKGEPGQGASVGHACSGARFRDVQVGVGKW"
     gene            complement(4420..6081)
                     /locus_tag="VHM48_03040"
     CDS             complement(4420..6081)
                     /locus_tag="VHM48_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754409.1"
                     /translation="MGRRFTLRLLVAALAVAGGLLLPAAVAAHPLGNFTINHYAGLTI
                     APDRVDLDVVIDMAEIPAFQERQDMDADGDGSVADDEAATWATGACTTLATSLHLTRG
                     GSAVALVAGAATVDFPPGAGGLSTLRLECAYTGVLAPVIASRVTITFADTSYAERIGW
                     REIGATGSGTILDTNGLPATSPSRKLTAYPADLIATPLDIRSASIDVRPDPAAAADSP
                     TPTSTPAPGAATGGAAAGPIGPPPTAAPVAAGAVPGGVAADLPDIFRTADLTPFVILA
                     SMLTAVAIGAGHALTPGHGKTLMAAYLVGSRGTAVHAVGLGLSVAVSHTLGILALAFV
                     IVGAGSILPPDVVYRVTPVIAGTSIVAIGGWMLSNEVRRRRARTTVVVAAEPAEAHEH
                     GHDHGSEAAHDHGHEGEHGHDDAHRHEPDHDAIPGEHSHGGLRHSHVPPAGATLSWRG
                     LFVLGLAGGLIPSTSALLILLGSIAAGRPAFGLVLVVAFGLGMAAVMTGVGLAMIFAR
                     TRLDRMPSRSSLGRLAAVAPLVASMAVLSLGLVLTWQAVAGAPVL"
BASE COUNT          895 a         2170 c         2253 g          847 t
ORIGIN      
        1 cccgaggaac cgttgcgtga cggggtccca aggaaacagg atcaggacct ggagcgagac
       61 cggcagcccg ctcgccggcg caccgcccca cggcccctcg ttcgtgccgg tcagggtcgc
      121 gacctcgaac acgccctgcg gcccgacgac gatctcggtc agcgcgaacc ggttgtccgg
      181 gaaggccgcg aagagctccg cgtagaacgc ccgcgccccc tcgtggccgg cccagcgctg
      241 accggtcggg atcatctcgt agacgcaggc cggcgccagg gtcgcgacca gcccttcgat
      301 gtcgcggcgg tcctcggcga tcgagtggcg gacccagagc cgcttgatcc ggcggagctc
      361 ggccgggtca aacgcacgag cgaggcgagc ctggatcgac ggattcggct ccgttcggcg
      421 gtcgtccatc tagacacccg ccgcgaggcg gacttccgca tcgcgagccc gggaccgccg
      481 ttcgatccgg atcatcacga ggatcaggac gatcgagccg atcgcgatca ggatggcggt
      541 cgcgatgtcg atgacgaacg ccgccgtgag cgagccgcga accgcgtcga ctgcgtcgcc
      601 aagagaggtc gcgttccgga tgtcgaatac cacccggagg gcgagatagc gactcgcgat
      661 cgccgacacg atccagctcc cgacgagtcc gacccaggcg agcccgacca tgaagaagcc
      721 gcccgccttc gggtcgaggc ggtacaggac gtcctggatc gtgcccggga cggtccgcag
      781 gttgagcacc gggatcagcg tcgtgacgaa cgctcgccac gggctggagc cgggaaggcc
      841 cccgcccagc gcaggcacgt tcgagacgac gcgactcagc cacgcggcga agaacagcaa
      901 cgtcggaagg atgatcgcga gccggacgag gccgaggctg ccggcactcg tcgcgacggc
      961 gttgaacgca gcctcgtcga tccggccggt cgccttgagc acgtcgagct gggtcatcgt
     1021 gaacgacatg atctcgacgg acttgagagc gaccgcgacg ctgaccgcgg ccagcccgag
     1081 caggaagatc gacgcagcca tgccgagcgc cgcggccggc cggtatggaa ccacatggcg
     1141 gctggcgatc gcctgttcct gccgatgcat cgccatctcg ccggtgtccg cggtcgcctg
     1201 ctcggcacca cacttgtagc agcgacgcgt ccgctggcgg ttgatcgagt ggcagttgcc
     1261 gcacaaccag atgtcggtca tcgtcggccc tcccgccgct ctcagtgctc ggtcgcgccg
     1321 gtgaacgtcc agccgtcgat ccggaccgcc ggcacgagga ccccgccgaa atcaccgcgg
     1381 accgtccgcc gttcgcgccc gaccgcgacc gtgccggcga gggcggccag atagctctgg
     1441 gtgaagcgca ggttgcggac cggcccgacg atcttgccgc cctcgacgag gaacgtcccg
     1501 tcgcgggtca tgccggtcac gatcgcgagc ttggggtgaa cggggttcgt gtagtggaag
     1561 cgggtgacga ggaggccgcg ctccatctcc ccgatgagcg cggcgcgctc ggtcgtgcct
     1621 gccgacatca ccatgttcag cgggaacggc ccgtacgagt tcggcgccgg caggccgtga
     1681 ccggtcgacg cgtggccgcc gcgcgcgccg gtctgggtgt cgtacacgac gtcgcggcag
     1741 acgcctcgct cgacgaggac gacgcgctgc ttggcgacac cctcgtagtc gaaggccatc
     1801 ggcatcgcgc cgggctccgt ggcgtcgtcg acgatcgtga cgagctcgct tccgatcacc
     1861 ttgccgggct cggcgaagga ccgctcctcc tggaccgcga gcgcgctgaa gcccatgaag
     1921 gcgagcagcg agaggagatc gacgacggcg tactcctcga gcacgaccga ccaatcgccg
     1981 ggctcgacgg cgacaggatt gacggtcgcc cgggccttct ccgccgcctc ccgcccgatc
     2041 gccgcggcat cgatcgccgt gacgtcgacc gacgcctgct cggcgtagcc cgatccgccg
     2101 tcggcgccca tcgtgaccgt cagaagctcg gcgctcgtcc gacgctgggc agcccggatc
     2161 ccgtgcgagt tggcgaccgc gaggtgctcc gccgccgtgg agaacgatcc gaaggcgcga
     2221 accccagccg cgtcggccgc ggcgatgacc gcccggaccc cggccgctcg ctgttcgggg
     2281 gtcgcggcgg ccgtcgcctc cgaccagccg gctggcagct catcgatggg cgtcggttcg
     2341 gggaggccgc cccagtcctc gagctcagcc gaattgcggg cgatcgcgac cgcggtttcg
     2401 gcgagacgac gcaggccgtc ctcgtcgagg cggttcgacg acgcgacccc gatccgcctc
     2461 ccgaacgcca cccgcaggtt gagcgtgcca ttcgtctcgg cgacgttctg gtggatctcg
     2521 ctgttggcga aacgggtcag ctgggcctgc tcggccgaga cgacggcctc cgcctcggtc
     2581 gcaccggccg cgtcggcgag ggcgacggcg cgggcggcga tcgctgcggt gtcgaacggc
     2641 gtcggcgccg aacgggctcc gtgggtcacg acacgctcac cacttgccca ccccgacctg
     2701 cacgtcgcgg aaccgtgccc cggagcacgc atggccgacc gaggcgccct ggcccggctc
     2761 gcccttgccg cagttcggcg tgcccagcat cacgtagctc gactcgtcgc cgaccgcgtc
     2821 gagcgaccgc cagaactcgt aggtgatccc ggtgtacgtc gggttcttga agagccggcc
     2881 cttcttgccg cccttgatct cccaggcgag ctcggtcgcg aactggaagt tgaggcggcg
     2941 gtcgtcgatc gaccacgagc ggttcgaggc gaggtacagg ccgtcgtcgg tggccgcgac
     3001 gatgtcgtcg aggctcagcc cgggcttcgg caggaggttc acgttcgtca tccggatcag
     3061 cgggatccgg ttccagccgt cggctcgcat cgcaccgccg gactgacgcc cgatccgcgg
     3121 cgcggtctcg cgactgctga gatagccgac gaagatcccg ttctcgacga ggggcacctg
     3181 ctgggcggca acgccttcgt catcccagcc gaacgtgccc aatccgccgg gcgcggtggc
     3241 gtcggcgacg atgtcgacga ggtcgctgcc gtagcggaag cccgcctcga gcttgtcggt
     3301 cgtcaggaag ctcgtcccgg cgtagctcgc ctcggtcccg aagacgcggt cgagctcggt
     3361 cgggtggccg cagctctcgt ggacctgcat gtagagctgg gacgggtcga ggatgatcgt
     3421 gaaccggccc gaggggcact gcggcgcact cagcagggcc accgcctcct cggccagtgg
     3481 tcccgcgtgg ccgacgaggt cgaggccgcg gacgtagtcg tagccggcgg cccggaatgg
     3541 gccgccgtcg gcgtcggggt aggtccgtcg ctggtgatcg tcgccctcga tcgcgttggc
     3601 ctcgatgttg gccccgacgt gggtgaacgt ctgctcggtc cagctcccgt cggtcgcccc
     3661 gaacgtccgc gtctcgcgga cggcgtcgta gcgggtctcg acgaaggcca cgccgtcgac
     3721 cgcccgggcc gcctcgtcgg ccgcgaggag gtggccgatc ttctcctcga gcggcaccgc
     3781 gaacgggtcc tcctcgatcg gtgtctcgta ggtcccgtga gcgggcggac ggtcgtcgag
     3841 ccggacacga tcgcgcagcg cggtcgcgct cgcctgcgcg atccggacgg cgtcggccgc
     3901 gacccgatcg gcctcgtcgg cggtcagtcg gctggagctc gcgaagcccc aggcgccgtc
     3961 gacgagcacc cggaccccga agccctcgct ctcaccggcg gcgacgccct cgacccggcc
     4021 gctcttcact gcgatcgatt cgtcgcgacg atggacgacg cggacgtcgg cgtaggacgc
     4081 gccgagctgg acggccgtat cgagggagcg gtcgctgacg tctcgcatcg gggcgggact
     4141 gtagcagagg tgggctgctc ccttcgctcg ggcgttcgcc ctatcgaggg ctgtcgcgag
     4201 cgcctgtcgt ggggcaaccg ggctggtttc ttgcgctcag gcgaggctcg cctccaatgg
     4261 gcggcgaagg gccgtttctc ttgcgcgcag gcgacgctcg cctgaggggc ggcgctgctg
     4321 gcgccgccgg agcgtggccg ccggccggat gcccgcggag ccgcacgaaa caggcgacgc
     4381 tcgcctgagc gcaggaaatc ccggacgtcg aattcgtcgt cagaggaccg gcgcgcccgc
     4441 gaccgcctgc cacgtcagga ccaggccgag gctgaggacg gccatcgacg cgacgagcgg
     4501 ggcgacggcg gcgaggcggc cgaggctcga gcggctcggc atccggtcga gacgggtgcg
     4561 ggcgaagatc atcgccaggc cgacgccggt catcacggcc gccatcccga gcccgaacgc
     4621 gacgacgagc acgagcccga acgcaggccg gccggccgcg atcgagccga ggaggatcaa
     4681 cagggccgac gtcgacggga tcagcccgcc ggccagaccg aggacgaaca gaccgcgcca
     4741 cgacagggtc gcgccggccg gcggcacgtg gctgtggcgc aggccgccgt ggctgtgctc
     4801 gccggggatc gcgtcgtggt cgggttcgtg ccgatgcgcg tcgtcgtgcc cgtgctcgcc
     4861 ttcgtgccca tggtcgtgcg cggcctcgga tccatggtcg tgcccgtgct cgtgcgcctc
     4921 ggccggctcc gccgcgacga cgacggtggt ccgcgcccgt cgccggcgga cctcgttgga
     4981 cagcatccag ccgccgatcg cgacgatcga ggtgccggcg atgaccggtg tcacccgata
     5041 gaccacgtca ggcgggagga tgctcccggc gccgacgatg acaaacgcca gggccaggat
     5101 gcccagcgtg tgcgacacgg caacggacag gccgaggccc actgcgtgaa ccgccgtccc
     5161 gcgcgagccg accaggtagg ccgccatcag cgtcttgcca tgccccgggg tcagagcgtg
     5221 tcctgccccg atggccacgg cggtcagcat cgaggcgagg atcacgaacg gcgtcaggtc
     5281 ggccgtccgg aagatgtcgg gcaggtcggc cgcgacccca cccgggaccg cgcccgccgc
     5341 caccggagca gcggtcggcg gcgggccgat cgggccggcc gccgccccgc ccgttgccgc
     5401 gcctggcgcg ggtgtcgagg tcggtgtcgg gctgtccgcg gcagcagccg ggtccgggcg
     5461 gacatcgatg gaggccgagc ggatgtcgag gggtgtcgcg atgaggtcgg ccggataggc
     5521 ggtcagcttc cggctcgggc tggtcgccgg caggccgttg gtgtcgagga tggtcccgct
     5581 gccggtcgca ccgatctcgc gccagccgat ccgctccgca tacgaggtgt cggcaaaggt
     5641 gatcgtcacg cgtgaggcga tcacgggcgc gagcacgccc gtgtaggcgc actcgagccg
     5701 gagggtcgag aggccgccgg cgccgggcgg gaagtccacc gtggccgccc cggcgacaag
     5761 cgccacggcc gacccgccgc gggtcaggtg caggctggtc gcgagggtcg tgcaggcgcc
     5821 ggtcgcccag gtcgcggctt cgtcgtcggc aacgctgccg tcgccgtccg cgtccatgtc
     5881 ctggcgttcc tggaatgccg ggatctcggc catgtcgatg acgacgtcga ggtcgacccg
     5941 atccggcgcg atcgtcagtc cggcgtagtg attgatcgtg aagttgccga gcggatgggc
     6001 ggccacggcc gccggcagca gcaacccgcc ggcgacggcc agggcggcga cgagcaggcg
     6061 gagggtgaag cggcgtccca tgtcgccgag ggtacgccga gcaccgccgg acagatgatg
     6121 ttcgatcgcc gatcagcggc gcgaacccgc aacggcaccg tacac
//
LOCUS       DATAIW010000090         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_93612, whole genome shotgun sequence.
ACCESSION   DATAIW010000090 DATAIW010000000
VERSION     DATAIW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93612"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHM48_03045"
     CDS             <1..463
                     /locus_tag="VHM48_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754410.1"
                     /translation="PGTARFERCAFDHAKLDGWRMSATELVGCRFVGPLRRMEFGGRP
                     VVNAVQRRERNEIRDNDFREADLHDVEFLGGVDLDAQLWPDDPTYLRLDVRPETLDRL
                     ARVVDRLPPEDRTPLADLAWVRGRFAGQSEILVREGTWQWYRRLLRLAAGN"
     gene            complement(634..1656)
                     /locus_tag="VHM48_03050"
     CDS             complement(634..1656)
                     /locus_tag="VHM48_03050"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2754411.1"
                     /translation="MHAIGVDTHKDSLAVCRVDELGAPLEERTVANDPDGHRAIHAWL
                     AEGSPAIVGIEGSSSFGAALARALVAAGMTVREVPPMLSHRERTRIRRAGKSDPGDAL
                     AIARVTLREPDLPPVRLVDRTLELRLLVEAREDLVAEQTRVRNRLHADLRALVPGYGS
                     VAANLIAVRHRESAARLLRPLRGVQAELARDRLARLRRLGFEVATLTKRITERTAGHP
                     LLAIAGVGDLVAAKLIGEAGDVTRFRSPDAFAALAGVAPVPASSGQTTHMRLDRGGNR
                     QLNRALYTIAMVQAAHAGPGRDYVRRKRAEGKSGRDAIRALKRQLVRTVFRRLQEGAR
                     AWAEAA"
     gene            2245..>2318
                     /locus_tag="VHM48_03055"
     CDS             2245..>2318
                     /locus_tag="VHM48_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754412.1"
                     /translation="MTGYATKEGDTGSPEVQIALLTER"
BASE COUNT          365 a          794 c          809 g          350 t
ORIGIN      
        1 cccgggcacg gcgcgattcg agcgctgcgc gttcgaccac gcgaaactcg acggctggcg
       61 gatgtcggcg acggagctcg tcggctgccg gttcgttggc ccgctgcggc ggatggagtt
      121 cggaggccga cccgtggtca atgccgtcca gcgtcgcgag cgtaacgaga tccgcgacaa
      181 cgacttccgg gaggcagatc ttcacgatgt cgagttcctc ggcggtgtgg atctcgacgc
      241 acagctttgg cccgacgacc cgacctacct ccggctcgac gtccggccgg agaccctcga
      301 ccgcctcgct cgggtggtcg atcggctccc gccggaggac cggacgccgc ttgctgacct
      361 ggcgtgggta cgcggacgtt tcgccggcca atccgagatc ctcgtcaggg agggcacctg
      421 gcagtggtat cggaggctgc tccggctcgc agcgggcaac tgatccgtcc ggacggcgtg
      481 ccgggcacgc tccacgtcgc cgacggtggc ctcctcttca tcgaagcacc gtcgacgacc
      541 cttacgcccg acccggtccg catggagctc cgcgcgtcgt cgatggacgc cgactctgag
      601 ctgtcggcgg tgccatgtgc aagctcctat ctgtcaagcg gcctccgccc aggcgcgagc
      661 gccttcttgc agccttcgaa agacggtccg gacgagttgc cgcttgaggg cccggatggc
      721 gtcgcgtccg gacttcccct cggcccgctt gcggcggaca tagtcccgcc cgggcccggc
      781 gtgggcggcc tggaccatgg cgatggtgta cagggctcgg ttgagctgtc gattgccgcc
      841 gcggtcgagg cgcatgtggg tcgtctggcc ggaactggct gggacgggcg cgacgccggc
      901 cagggcggcg aacgcatcag gcgagcggaa gcgggtcacg tcgccggctt cgccgatgag
      961 cttggccgcc acgaggtcgc cgacaccggc gatcgcgagc agcggatgac ccgccgtccg
     1021 ctcggtgatc cgtttggtca acgtcgccac ctcgaacccg agccgccgca ggcgggccaa
     1081 ccggtcacgc gcgagttcgg cttgcacccc gcgcagcggc cggagcagcc gagccgccga
     1141 ctcccggtga cggacggcga tgaggttggc agccacgctg ccatacccag gcaccagcgc
     1201 tcgcagatcg gcatggagcc tgttgcggac gcgggtttgc tcggccacga ggtcctcgcg
     1261 cgcctccacg aggagccgca gctcgagggt ccggtcgacc agccggaccg gcggcaggtc
     1321 gggttcgcgc agcgtgaccc gggcgatcgc cagggcatca ccggggtcgc tcttgccggc
     1381 ccgccggatc cgggtgcgct cgcggtggga gagcatcggt ggcacctcgc ggacggtcat
     1441 gcccgccgcc accagcgcgc gagccagagc ggccccgaag ctcgacgagc cttcgatccc
     1501 gacgatcgcc ggcgatccct cggcgagcca ggcgtggatc gcccgatggc cgtccgggtc
     1561 attggcaacg gtccgctcct cgagcggcgc cccgagttca tcaacgcggc agacggccag
     1621 gctgtccttg tgcgtgtcga ccccgatcgc atgcatctcg cgcccctccc tggtctcgat
     1681 accatgagcg cgggccggcc aggcagatgt gcgttgagtc aaagccacgc tcctatgaag
     1741 ccatggccgg cccgctcgtt cgaggcgacg ggggcacttg cacagaaagc cagaccctca
     1801 ttcgggtcga cgaatcaaat gatgagccac ccgtcgctcg agcgaacctt cccactcgaa
     1861 aggccggtcg agcctgacgc atatcagatg aaccgatggg acgtcccgga cccctgtggt
     1921 gccccgagcg gtgcgaaatg gcgcatcgga tgcacgcctc ggatcgaatt cggccgcatc
     1981 ggcggcgaat cggcgctcca atcggtccaa tcggtcgcgt aggtgcaagc tgatgaacgc
     2041 gaatcccgga atggcgcatc cgatgcacct cggcggcgac ggcggagacc gtggcgacgg
     2101 ccgcgacggc ccgttcggat catcgccgtt tgcccggccg gcgcgactcg tgctaccgtt
     2161 cgccggtcat agaagaaaga caacgataga agttcgtgtt cggaggaacg agtgccgctc
     2221 gcgcgggaac agaagcagga gatcatgacc gggtatgcca ccaaggaagg tgacaccggg
     2281 tcgccggagg tccagatcgc gctcctgacg gagcgaat
//
LOCUS       DATAIW010000091         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_34102, whole genome shotgun sequence.
ACCESSION   DATAIW010000091 DATAIW010000000
VERSION     DATAIW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34102"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VHM48_03060"
     CDS             <1..649
                     /locus_tag="VHM48_03060"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2754413.1"
                     /translation="RISIAELAATAHGGALLLARGSGMPPDAPEHDASACVGRLGFES
                     FASPTFFCHAVRIRVDPATGIVRALKVVAATDVGRVLNETGALGQMTGGVVMALGNAT
                     LEGTVFGADGRQRNASLLDYKLLTTADAPEIEAIFVEHPASNGGPRGSKGVGEPPIVA
                     TAGAVANAIASATGVRVRRLPMTPERIWRAVTTGDDGAAEANAPFGPAAVVAANR"
     gene            646..1560
                     /locus_tag="VHM48_03065"
     CDS             646..1560
                     /locus_tag="VHM48_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX2754414.1"
                     /translation="MTSRFAAPATLEAALAILAADGAARPVAGGTDLVVAARQGRKDL
                     PESIVAIDRITELARHEVVDGVLVLGALTSHDWLAGSPDVGSAWSALADASAIVGSPA
                     TRATGTIGGNLMNASPAAETTAPLVVLDAAVTLQAAGGASRAVAVEDLATGPGRTVAG
                     AGELLTGVAVPPTAAGSGSAYIRLEYRRAMEIAVVGAAALVTLTGDGRIAAARVALTA
                     VAPTIVRAPEAEAALIGATADPATFRIAAAAAAAAARPIDDVRASADYRRAMLVVVVA
                     RTLAAAAARARGQSIPIPASRWAPGQEV"
     gene            1564..2082
                     /locus_tag="VHM48_03070"
     CDS             1564..2082
                     /locus_tag="VHM48_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2754415.1"
                     /translation="MPELLQLTVDGLPLSAVVEPGRTLLRVLRDDLGRTGTKEGCDDS
                     ECGACMVLIDGRPVNSCSYLALQAAGREITTVEGLAGVEALHPLQQAFLEGGGIQCGF
                     CTPGMLISAVALLARDPDPSEEAIRDGLAGNLCRCTGYQPIIRAVQRAAAEMRAAVSA
                     GAVTASLVDPAL"
     gene            complement(2161..3603)
                     /locus_tag="VHM48_03075"
     CDS             complement(2161..3603)
                     /locus_tag="VHM48_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2754416.1"
                     /translation="MTPTFGWSLDFAGRRRLSIALPIVLLTAVFGATGVQAAGYDAAS
                     DVNSMALTTEFTGAQAWWAAGYTGQGIDVALIDSGVSPVEGLSTAGKIVYGPDLSLES
                     QAPNLTNLDTFGHGTFMAGLIAGRDTSLTAPYQQAPASTYRGMAPDARIVSLKVATAD
                     GGVDVSQVIAAIDWVVQHRNDNGLNIRVLNISYGTNSSQWYGVDPLAYAVEQAWDAGI
                     VVVAAAGNSGFQARGSSPALANPAFDKRIIAVGASDSMGTAALSDDTVPDFSAAAKTG
                     SARAPDFVGPGVHLQGLRVPNSWIDTQVDGKTVLDGRFMRGSGTSEAAAIASGSAALI
                     LSKYPNATPDQVKRLIKITAYDMPQCGRTGTMGSGELRLGAMLSTSLPNYSQSIAPAT
                     GGGTVEQARGSDHLTRDGVVLKGEQDIFGKAFNGTAMAVLEAAGNSWSGGTWNGSTWT
                     GSSWSGNSWSGSSWSGNSWSGNSWSGADWN"
     regulatory      complement(3744..3833)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3985..>5899)
                     /locus_tag="VHM48_03080"
     CDS             complement(3985..>5899)
                     /locus_tag="VHM48_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2754417.1"
                     /translation="AYVSERQQHEGLEMLYESTRILQRSPQIDQALVSLLDHARKMFR
                     AEVAEVSLLPQVEGQEILRTRVGPGGAIDMMKPIGTALDDPRLVQCVVERRAFLVAGE
                     PTPASAAGGRFRNAIYAPLVGESRLVGTILVANRLSDLSPFDADDLKLFQTLASHTAV
                     ALENGQLEQSLSSLSRLQDELQHKAYHDALTGLANRAFFAQSVAQRLESADASGLVPV
                     VLFLDLDDFKIVNDTLGHAAGDELLASVGERIQATVRSGDLAARIGGDEFAVLLWDTP
                     EMPVARRIAERLIEAFGAGASDDGAAANAPVSIGVAAGHPGWGSADELLRNADVAMYS
                     AKARGKNRIAFFEPEMATAVAARHTLTESLQRAVAAEEFVLHFQPIFEVATGRMTGVE
                     SLVRWMHPTRGMVWPGEFIPVAEASDLILDIGRWVLDRSCQQAKEWHDRWPELHDLQI
                     SVNVAGRQLEQPDFVDQVAKIVRSSGVDPATVVLEVTETTLLQNAEDAIAKLEALRAL
                     GLGVAIDDFGTGYSSLSYLQRFPATAIKIARDFIAVDEADADSWELASAIVSMGRALR
                     LTVIAEGVEEPFQLERLRKLRCSRAQGYYLALPLPAEELEELFRQPRTSIVWSRSAAP
                     ELVNLSSRDTPAA"
BASE COUNT          821 a         2081 c         2100 g          897 t
ORIGIN      
        1 acggatctcg atcgccgagc tcgcggcgac ggcccacggc ggcgctttgc tgctcgcccg
       61 cggctcgggg atgccacccg acgctcccga acatgatgcc tccgcctgtg tcggccgtct
      121 cgggttcgag tcgtttgcgt ctccgacgtt cttctgccat gccgtccgga tccgggtcga
      181 ccccgcgacc gggatcgtcc gggccctcaa ggtcgtggcc gcgaccgacg tgggtcgcgt
      241 cctgaacgag accggagccc tgggccagat gacgggcggg gtcgtgatgg ccctcggcaa
      301 cgcgacgctc gaggggaccg tcttcggggc cgacggccgc cagcgcaacg cgtcgctgct
      361 cgactacaag ctgctcacga ccgcggacgc acccgagatc gaggcgatct tcgtcgagca
      421 tccggcctcg aacggtggcc cgcgcgggtc gaagggcgtc ggcgagccgc cgatcgtggc
      481 gacggccggc gccgtcgcga acgcgatcgc cagcgcgacc ggtgtccgcg tccgccggct
      541 gccgatgacg ccggagcgca tctggcgcgc ggtcacgacc ggcgatgacg gcgccgccga
      601 agccaacgcg ccgttcgggc cggccgcggt cgtggcggcc aaccgatgac aagccggttc
      661 gcggcccccg ccaccctgga ggccgcgctc gcgatcctcg ccgcggacgg cgccgcccgc
      721 ccggtcgccg gtgggacgga cctcgtcgtc gccgctcgcc agggtcggaa ggacctcccg
      781 gagtccatcg tcgcgatcga tcggatcacc gagcttgccc ggcatgaggt cgtcgacggg
      841 gtcctcgtcc tcggtgccct gacgtcccac gactggctcg ccggttcgcc cgacgtcggc
      901 tctgcgtgga gcgccctcgc cgatgcgtcc gcgatcgtcg gttcgcccgc gacccgcgcg
      961 accggaacga tcggcggcaa cctgatgaac gcatcgcccg cggccgaaac gacggccccc
     1021 ctggtcgtgc tcgatgcggc tgtcaccctg caggcggcgg gcggcgcctc gcgggcggtt
     1081 gccgtcgagg acctcgcgac cggacccggg cggacggtgg ctggggcagg ggagttgctc
     1141 acgggtgttg ccgtcccgcc gacggccgcc ggcagcggca gcgcctacat ccgcctcgag
     1201 tatcggcgag cgatggagat cgccgtggtc ggggcggccg ccctcgtcac gctgaccggc
     1261 gacggccgga tcgcggccgc ccgcgtcgcc ctgaccgccg tcgctccgac gatcgtgcgc
     1321 gccccggaag ccgaggccgc gctgatcgga gccaccgccg acccggccac gttccgcatc
     1381 gccgcggccg cggccgcggc cgcggcccgc ccgatcgacg acgtccgggc cagcgccgac
     1441 taccgccggg cgatgctcgt cgtcgtcgtc gcccggaccc tcgccgcggc cgccgctcgg
     1501 gcccgcggcc aatccatccc gatcccggcc tcgcgctggg cgcccggcca ggaggtctga
     1561 cccgtgcccg aattgctcca gctgaccgtc gacgggttac cgctcagcgc ggtcgtcgag
     1621 ccgggccgga cgttgctgcg cgtccttcgc gacgatctcg gtcggaccgg caccaaggaa
     1681 ggctgcgacg actccgagtg cggagcctgc atggtcctca tcgacggccg gccggtgaac
     1741 tcgtgctcgt acctcgccct ccaggccgcc ggccgcgaga tcacgaccgt cgagggcctc
     1801 gccggcgtcg aggcgcttca ccccctccag caggcgttcc tcgagggcgg cgggatccag
     1861 tgcgggttct gcacgcccgg catgctgatc tcggcggtgg cgctgctggc ccgcgatccg
     1921 gacccatccg aggaggcgat ccgggacggg ctcgcgggca acctctgccg ctgcaccggc
     1981 taccagccga tcatccgtgc cgtccagcgg gcggcggccg agatgcgcgc cgccgtgtcg
     2041 gccggcgccg tcaccgcgag tctggtcgac ccagctctgt aacgcagcgg atccgggcat
     2101 gccgggccag cgcaaccgac gggcggcgcc ggcctcggag cgatctcctt gccggttcgg
     2161 ttagttccag tcggcgcccg accagctgtt gccggaccaa ctgttgccgg accacgagga
     2221 tcccgaccag ctgttgccgg accacgagga gccggtccag gtgctgccgt tccacgtccc
     2281 gccggaccac gagttgccgg cggcctcgag aaccgccatc gccgttccgt tgaacgcctt
     2341 tccgaagatg tcctgctcgc ccttgaggac cacgccgtcc cgggtcaggt gatccgaccc
     2401 gcgcgcctgc tcgacggttc cgccacccgt cgcgggtgcg atggactggg agtagttggg
     2461 gagcgaggtg gagagcatgg ctccgaggcg gagctcgccg gatcccatcg tgccggtccg
     2521 gccgcactgg ggcatgtcgt acgcggtgat cttgatcagc cgcttgacct ggtcgggcgt
     2581 cgcgttcggg tacttgctca agatgagggc ggccgatccc gacgcgatcg cagccgcctc
     2641 ggaggtgcca ctgccccgca tgaaccggcc gtcgaggacc gtcttgccgt cgacctgggt
     2701 atcgatccag gagttcggga cgcgcaggcc ctggaggtgg acgccggggc cgacgaagtc
     2761 cggggcgcgc gccgagccgg tcttcgcggc agccgagaag tccggcaccg tgtcgtcgga
     2821 gagcgctgcg gtgcccatcg aatccgatgc gccgacggcg atgattcgct tgtcgaacgc
     2881 ggggttggcg agagccggcg aggatcccct ggcctggaac ccgctgtttc cggcggccgc
     2941 cacgacgacg atccctgcgt cccaggcctg ttcgacggcg taggcgagcg ggtcgacgcc
     3001 gtaccactgt gaggagttcg taccgtagga gatgttgagg acacggatgt tcaggccgtt
     3061 gtcgttgcgg tgctggacga cccagtcgat cgccgcgatg acctgcgaga cgtccactcc
     3121 accgtcggcg gtcgccacct tgaggctgac gatgcgggca tccggtgcca tgccgcggta
     3181 ggtcgacgcc ggtgcctgct ggtacggagc ggtgagggac gtgtcccgac cggcgatgag
     3241 gccggccatg aaggtgccgt ggccgaacgt gtcgaggttg gtcaggttgg gtgcctggga
     3301 ctcgagcgac aggtcaggcc cgtagacgat cttgccggcc gtgctcagtc cctcgacggg
     3361 ggagacgccg gagtcgatca gcgcgacgtc gatcccctgg cccgtgtacc cggcggccca
     3421 ccatgcctgc gcgcccgtga attccgtcgt cagtgccatc gagttgacgt cgctcgcggc
     3481 gtcgtagccg gccgcctgaa cgcccgtggc cccgaagacc gcggtcagga gaacgatggg
     3541 gagggcgatg ctcagccggc ggcggccggc gaagtcgagg ctccagccga acgtcggcgt
     3601 catcgggccg cgctcgctgg tcggatccgg acaccggcac ggccggcgtt gtccaggtgg
     3661 cgtgtctgcc ctggttcgtc caaggctgtg cgctcgggct gcaccatcga agtccacctt
     3721 ccggggggct tgttgatgcg gcgcctggca accgggcggt cttgtcgaag cgtcttcgaa
     3781 cgagatccct ggctttgcgt ccccgcctca cggcgggttt gccttgtttc agaccggatc
     3841 gtgcgacagc ccatctggct gccgacaccg cggaccgtag gccgccattc gccggcccca
     3901 aatgggcgaa aggtcccgtt cggtgcgtcg gcgcatagcc cattcggcga ccgatctctg
     3961 cggccgtgtg acggcgacga gtcgtcaggc cgcgggcgtg tcccgcgacg acaggttgac
     4021 cagctcgggg gcagcggagc gggaccacac gatcgacgtc cgcggctgcc tgaacaactc
     4081 ctcgagctcc tcggcgggga gcggtagagc gaggtagtag ccctgggctc gactgcagcg
     4141 cagcttccgc agccgctcca gctggaaggg ctcctcgacg ccctcggcga tgacggtcag
     4201 ccgaagcgcc cgtcccatcg acacgatggc gctggcgagc tcccagctgt ccgcgtcggc
     4261 ctcgtcgacc gcgatgaagt cgcgggcgat cttgatcgcc gtcgccggga agcgctggag
     4321 gtagctgagc gaggagtagc cggttccgaa gtcgtcgatc gcgacaccga ggccgagggc
     4381 gcgaagcgcc tcgagcttgg cgatcgcatc ctccgcgttc tgcaacaggg tcgtctccgt
     4441 cacctcgagc acgacggtcg ccgggtccac cccggacgat cgaacgatct tggcgacctg
     4501 gtcaacgaag tcgggttgct cgagctggcg gcctgcaacg ttcacgctga tctggaggtc
     4561 gtgcagctcc ggccagcggt cgtgccattc cttggcttgc tggcaggagc gatcgagcac
     4621 ccagcggccg atgtcgagga tcaggtctga cgcttccgcc acggggatga actcgcccgg
     4681 ccagaccatg ccccgggtgg gatgcatcca gcgcacgagc gactccacgc cggtcatccg
     4741 gccggtcgcg acctcgaaga tcggctggaa gtgaaggacg aactcctcgg ccgcgaccgc
     4801 tcgctggagc gactcggtga gcgtgtgccg ggccgcgacg gccgtcgcca tctccggctc
     4861 gaagaacgcg atccggttct tgccacgcgc cttcgcggag tacatcgcca cgtccgcatt
     4921 ccggagaagc tcgtcggccg acccccagcc cgggtggccg gccgcgaccc cgatgctcac
     4981 gggagcgttc gcggctgcgc catcgtccga cgcccccgcc ccgaacgcct cgatcaggcg
     5041 ttcggcgatg cgccttgcga cgggcatttc cggggtatcc cagaggagca cggcgaactc
     5101 gtcgccaccg atccgggcgg caagatcgcc cgaccggacc gtcgcctgga tgcgctcgcc
     5161 caccgacgcc aggagctcgt cgccggccgc atggcccagg gtgtcgttga cgatcttgaa
     5221 gtcatcgagg tcgaggaaca ggacgacggg gaccagcccc gaggcgtcgg ccgactcgag
     5281 tcgctgggca acgctctggg cgaagaacgc gcggttggcg agcccggtca atgcgtcgtg
     5341 ataggccttg tgctggagct cgtcctggag tcgtgaaagc gagctcaggg actgctccaa
     5401 ctggccgttc tcgagcgcga ccgccgtgtg gctggcgagc gtctggaaga gcttgagatc
     5461 gtcggcgtcg aacggactga ggtcgctcag gcggttggca accaggatcg tcccgaccag
     5521 gcgcgactcg ccaacgagcg gcgcgtagat cgcattgcgg aagcgaccgc cagccgctga
     5581 cgcaggtgtc ggctccccgg cgacgaggaa ggcccgacgc tccacgacgc actgcaccag
     5641 gcgcgggtcg tcgagcgcgg tcccgatcgg tttcatcatg tcgattgccc cgcccggacc
     5701 gacgcgtgtc cgcaggatct cctgaccttc gacctggggc agaagggaca cctccgcaac
     5761 ctcggcccgg aacatcttgc gggcgtggtc gagcagggac acgagagcct ggtcgatctg
     5821 ggggcttcgc tgcaggatcc gggtggactc gtagagcatc tccaggcctt cgtgctgctg
     5881 gcgctccgac acgtacgcg
//
LOCUS       DATAIW010000092        10847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_93646, whole genome shotgun sequence.
ACCESSION   DATAIW010000092 DATAIW010000000
VERSION     DATAIW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93646"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1227)
                     /locus_tag="VHM48_03085"
     CDS             complement(367..1227)
                     /locus_tag="VHM48_03085"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX2754418.1"
                     /translation="MRLVRTELVDSREILPGQWLQSFLAPELATGARAGQFVHARTGD
                     LSGLLLRRPFSINTADAATGVITIHFRKIGRGTEWFTRLRPGDAVDMLGPLGRPFEVD
                     PRSRHLLLIAGGLGMAGVRSLVDEALRDGRQVTVLFGAASARDVYPSSLLPDEVEYII
                     ATDDGSVGHRGFITELVPAYEAWADQAFACGPAPMLAALARLAAGRRERLGVAKLGRK
                     RGGGKPDAVGSPAARRKAFLQVSMEQNMGCAVGACLGCVIMGSNGQPQRVCREGPVFA
                     AEEVAWESGW"
     gene            complement(1230..2252)
                     /locus_tag="VHM48_03090"
     CDS             complement(1230..2252)
                     /locus_tag="VHM48_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754419.1"
                     /translation="MDSSRNDLLGEIAGDAALERSDFIVQSTEQLRKFLNANKDRINE
                     LGGLVLIDDDPDYLSIAPDLTFRSRTRYLDELTGEWTSETEVIENASELVELYNPADI
                     YQAFAEAAREAAGLEAEPTAEGDLLDVAGIGTDESVTIGAHDPYAAAADEWAATQPEP
                     VVVDDDELAAQRLYDLALEFQERSQRSEARLLEQFEDASSVLIGKIGDLVVNDDDDER
                     LTLTASGEFRAEVLTEEDNEWRPLTTAEELVEYYDPTDVFGDLADALAEAFPSIAPEI
                     AAFEADAEGEAAKGESDDDEGDEGDEGEDDEGDDEGEDDDDKDDKDDDKAEADGSKPA
                     EGDATR"
     gene            complement(2319..2645)
                     /locus_tag="VHM48_03095"
     CDS             complement(2319..2645)
                     /locus_tag="VHM48_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754420.1"
                     /translation="MPVLATYFSVRRGRDAFFKFYMRTLFARQVREYETKYGIRFLGW
                     YNVAHGWDFDNVILLDLPDYATLDKLEADDGLRALGHRAGEWIFQRHHSMFLRERMGP
                     DLEYHG"
     gene            complement(2716..3120)
                     /locus_tag="VHM48_03100"
     CDS             complement(2716..3120)
                     /locus_tag="VHM48_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754421.1"
                     /translation="MTINAKPVQYKGDDLDPERGPGLGCFRFQLIVLGIFIVLTPLSV
                     VWQWPDAVSAALLFSVVILLLFTGQTIIFLLRLVAADRRGRRRPLGSATMTVGELEDA
                     TDDADSPPTDDAESPPDDDAESPPDDEGAVRQ"
     gene            3257..4573
                     /locus_tag="VHM48_03105"
     CDS             3257..4573
                     /locus_tag="VHM48_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2754422.1"
                     /translation="MSRQRWLIIALPALVVGIIEALSDTILDPVLPFPGDALIVTLAV
                     LLLSIAVSGLAFGRIDALGAALAARNRELEMRAGSAAAMHKVSVAITALVDLRSILDT
                     IVDNARTLLAADVAVILLVDADGALRRSASSGPVRETGDLAERSGADPDDLLQFLPAD
                     LATSRLANALNRGPETIGLLAIGSKRARSFDAEEVETLASLANQASIAIENARLQARL
                     RELAVETERGRIAREMHDGLAQVLGYVSTKSQAADELLAVGRVADARAQLGELTAAAR
                     SIYVDVREAILGLRSPITPELGLVGAIEDYARRFADASKLAVTVEASSEARAATMGPE
                     AEAQVFRIVQESLTNVRKHAAAGRVAVHLGRVDDDLLVELDDDGRGFDEPSPWQSTDW
                     PHYGQAAMQERAADIGARISWGTRPTGGGFIRLVVPINGFILEVAT"
     gene            4573..5214
                     /locus_tag="VHM48_03110"
     CDS             4573..5214
                     /locus_tag="VHM48_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2754423.1"
                     /translation="MRVLLADDHALVRDGIASLLSAWGHDVVGQAANGEDAVALADRL
                     EPDLILMDVRMPGSSGLEATRRIAGRHPAIAIVMLTVSEDEDDLFEAIKAGARGYLLK
                     NLEARELRAMLEAIARGDAAISPATAFRIIREFARGPVTDAVDTPTALTARELDVLGL
                     VTAGLRNKEIAARLGISENTAKFHLRNILGKLHAESRTELATRALRERLVPDD"
     gene            5346..7331
                     /locus_tag="VHM48_03115"
     CDS             5346..7331
                     /locus_tag="VHM48_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754424.1"
                     /translation="MGYRIDIDHGGCINCGICMDVCPVEALDMSRPERPGIESGQGFG
                     QPFVWSMEHPVQVGECIGCTVCIEQCPVTVMTLATQPGPTDLAPRQGPIHRPLPESGG
                     WVPLSAVTLESLKPAHPSPWSDVIGVRTPSRWPAGSPLPHDTAPHQAPCQVACPAGTD
                     AGRYVGLVAQGRYDDAYAVAAEVNPFPSVCGWICTAPCEAACRRGVLDEPISIRTIKR
                     FAAEHGSLPAVKPPTTRRPERVAIVGGGPAGMAAAWYLARLGYGVTVLEAMPVPGGMM
                     AIGIPAYRLPRDVLQAEIARIVSVGVELRLNAVMGRDFTLGDLEREGYRAVFLATGAS
                     KSRRLGIPGDDLTGVVPATLFLKRVNLGEEPTLSGDVVVVGGGSTAMDAARSALRSGA
                     ASVTVAYRRTRADMPAQVEEIEAAEREGITIRTGLTPAEVVGRDGQVVALRCRPTAAG
                     GEQAPLDGQVTVDSEAVAVDLPGRTILVAIGEEPDPSILPEGSGIEVSAWAGVVADSR
                     TLATGRAGIFAGGDVVSGAKTIIDAVAAGRRAAGSIHEYLAGVHDGEADILRTVRYAT
                     APEHDLVLDLEPRPRLHAPLPVVDTRSFAPAHPGFDPAMARAEAGRCFRCDAVEVCTT
                     VHVTGGRGPADRPGLTPGRQPTTPSQAQQPTTTGGVA"
     gene            7328..7960
                     /locus_tag="VHM48_03120"
     CDS             7328..7960
                     /locus_tag="VHM48_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754425.1"
                     /translation="MTPDQVSGFFDAGENFVEGTIAAALVVLWMLAVALHLARPYMVR
                     NTGKFSLRLGADLWWIIYVGLRDIILVQVLLGSFIFFYPDVVAGQDLPITGGLAAVCA
                     FAALLIKLTTAGDRDINGYRWQVILIGVGAALYIGPYFLGTQMTELTGQRVDTIMPFL
                     VSSKNLDLALPLCYLSGALAGACGVIAVLYNLRGTGRRPAAMTTTEENAR"
     gene            7957..8595
                     /locus_tag="VHM48_03125"
     CDS             7957..8595
                     /locus_tag="VHM48_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754426.1"
                     /translation="MNADDLATALTTGASGWLVLSVAAVLPILWAFTLVLHFARPYVI
                     RFLGGLTLRFGGDVWWLSYVLFRDAILVITLGLSMIFLMPNLYLKVGLPITAPIATVV
                     LFWALLVKLVRDTDDDPGAFRIFSVLLVIASILYIVPQVYGLEAADQEYLGSLPATLI
                     SSENLDLARPILWISLALFAATGAGLFTRFLVMLGRADGPAEEAAQPGSTAG"
     gene            complement(8666..10024)
                     /locus_tag="VHM48_03130"
     CDS             complement(8666..10024)
                     /locus_tag="VHM48_03130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2754427.1"
                     /translation="MALTSARRGSALNRDYERRPYWHDTMPVLPSRRDRQLPDSADVV
                     VIGGGYVGINAARVLARGGAAVTLLEAEHLGFGGSTRNGGIVHPGFKWGPQALIERYG
                     DETGRALFGETMAGYELVKRLIADEQIDCEFRERGFLDLAWAPSHADDLRDSVATLAR
                     FGVEAEYIPKERLHEEIGTTFYHGGLVFPPSGLLHPGKYFAGLAAAADAAGADLHEGV
                     RARTIRRQADGRLVVETDRGAILTNEVIVGTNGYTDGVVPDLRRRVIPIGSYIIATEP
                     LPEELARTLSPKGRAFFDTKNFLYYWHVSEDRRMIFGGRASFLPTSVDRTAGILHKGM
                     LEVHPQLAGYRVEYAWGGNVGFTFDRMPHVGRKDGITYAMGCCGTGVALMTALGTAVG
                     EWLGGGPAPALSKLKFPLVPAPYEGHPWFLPFAGEWFRLQDRLARRGSGLAHVPETEP
                     GA"
     gene            10129..10707
                     /locus_tag="VHM48_03135"
     CDS             10129..10707
                     /locus_tag="VHM48_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2754428.1"
                     /translation="MGERVRRDPRIGPAIDRSLIDRARNGDLDAFESIVNARMDAVYR
                     LTSAILGNEADARDAAQETFVAAWRQLARLREPEKFEAWLQRIAINAARQTMRARSRR
                     RIREIPSSTVVALAERVAGGPRDDAAILDAALATMPIDQRAILVLHHLEGRSITELAA
                     ILDIPEGTAKSRLFAARRALAAAITASEAGGR"
BASE COUNT         1563 a         3851 c         3793 g         1640 t
ORIGIN      
        1 ggcacccgga cagcctcgat gcgcggcgtg gcggggcgtg gcggtcgcgt ggacaaggcg
       61 ggccccgccg cagcagccga cgactgtttt cgccgcggcg tcggtccgcg ctggatcccg
      121 cccggccgga cgtccttcga cgtcgtgact cgccgcttcg ccttcacccc gcgcctcccg
      181 accccatcag gttcgccggg cggaccaagg agcgaacgga tcgtgcgccg gccatcgatt
      241 cgcgctgctc ggccgtccgg tggtccggca ggcggaccag accgcgagga acggccgcgc
      301 cggccgcccg ttggtccggc aggcggacca gaccgcctgg tcgggagggc gcgggacggg
      361 gcgcgctcac cagccggact cccaggcgac ttcctcggcg gcgaacaccg ggccctcgcg
      421 gcagacccgt tgcggctggc cgttgctgcc catgatcacg cagccgagac acgctccgac
      481 cgcgcagccc atgttctgtt ccatcgagac ctggaggaac gctttgcggc gggcggcggg
      541 cgagcccaca gcgtccggct tgccgccgcc ccgcttgcgc ccgagcttgg cgaccccgag
      601 ccgctcccgc cgcccggcgg caagccgggc gagggccgcg agcatcggcg ccgggccgca
      661 ggcgaacgcc tggtcggccc acgcctcata cgcgggcacg agctccgtga tgaaccctcg
      721 gtggccgacc gatccatcgt cggtggcgat gatgtactcg acctcgtcgg gcaggagcga
      781 cgacggatag acgtcgcgcg ccgatgccgc cccgaacagg acggtgacct ggcggccgtc
      841 gcggagcgcc tcgtcgacaa gcgagcggac gccggccatc ccgagcccac cggcgatcag
      901 gaggaggtga cgcgagcgcg gatcgacctc gaacggccgg ccgagggggc cgagcatatc
      961 gaccgcgtcg cccggccgga ggcgggtgaa ccattcggtc ccgcgaccga tcttccggaa
     1021 atggatcgtg atcactccgg tcgcggcatc cgcggtgttg atcgagaacg ggcggcgcag
     1081 gagcagcccg gacaggtcac cggtccgggc gtggacgaac tggccggccc gggcaccggt
     1141 cgcgagctcg ggagcgagga acgactggag ccattggccc gggaggatct cccggctgtc
     1201 gacgagctcg gtccggacca ggcgcatggt caccgggtgg cgtcgccctc cgccggcttc
     1261 gacccgtcgg cctcggcctt gtcgtcgtcc ttgtcgtcct tgtcgtcgtc gtcttcgcct
     1321 tcgtcgtcgc cttcgtcgtc ttcgccttcg tcaccttcgt cgccttcgtc gtcgtccgac
     1381 tcgcccttgg cggcctcacc ctcggcgtcg gcctcgaatg ccgcgatctc cggcgcgatc
     1441 gacggaaacg cctcggcgag cgcgtcggcg agatcaccga agacgtcggt cgggtcgtag
     1501 tactcgacga gctcctcggc ggtcgtcagc ggccgccact cgttgtcttc ctccgtcagg
     1561 acctcggccc ggaactcgcc cgatgcggtc agggtcaatc gctcgtcgtc gtcgtcgttg
     1621 acgaccaggt cgccgatctt gccgatcagg accgacgacg catcctcgaa ctgctcgagg
     1681 aggcgagcct cgctgcgctg gctccgctcc tggaactcga gggcgaggtc gtacaggcgc
     1741 tgggcggcga gctcgtcgtc gtcgaccacg accggctccg gctgggtcgc ggcccactcg
     1801 tcggcggccg cggcgtacgg atcgtgagcg ccgatggtga cgctctcgtc cgtcccgatg
     1861 ccggcaacat cgagcaggtc gccttccgcc gtcggctccg cttcgagacc ggcggcctca
     1921 cgggcggcct cggcgaaggc ctggtagatg tcggccgggt tgtagagctc gacgagctcg
     1981 gatgcgttct cgatgacctc ggtctccgac gtccattcgc cggtcagctc gtcgaggtag
     2041 cgggtccggc tgcggaaggt caggtcgggc gcgatcgaga ggtagtccgg gtcgtcatcg
     2101 atgaggacca gaccgccgag ctcgttgatc cggtccttgt tggcgttgag gaacttgcgc
     2161 agctgctcgg tcgactggac gatgaaatcg gagcgctcga gggcggcgtc gcccgcgatc
     2221 tcaccgagca ggtcgttgcg gctcgagtcc atcgcggttc gcctcccatc gttcggttgt
     2281 ggtgttgcag tgcgtcggat cgacatcggg ctcccgggct agccgtggta ctcgagatcg
     2341 gggcccatcc gctctcgcag gaacatcgag tgatgacgct ggaagatcca ttcgccggca
     2401 cggtggccga gggcgcgaag gccatcgtca gcctcgagct tgtccagtgt cgcgtagtcc
     2461 ggaagatcga gcaggatcac gttgtcgaaa tcccagccgt gggcgacgtt gtaccagccg
     2521 agaaagcgga tcccgtactt ggtctcgtac tcgcgcacct ggcgggcgaa gagggtccgc
     2581 atgtagaact tgaaaaaggc atcccgaccg cggcggacgg agaagtaggt cgccagaacg
     2641 ggcacggttc tgcggtcctc cactccagcg atccagggcg cgaatcgcgc ccggcccggc
     2701 aggcaccggg gtcgcctatt gtcgcacggc cccttcgtcg tccggaggcg actcggcgtc
     2761 gtcgtccgga ggcgactcgg cgtcgtcagt cggcggtgag tccgcgtcgt cagtcgcgtc
     2821 ctcgagctcg ccgaccgtca tcgtcgcgct ccccagcgga cggcgccggc cacgccgatc
     2881 ggcggccacg aggcgaagca ggaagatgat cgtctgaccg gtgaacagga ggaggatgac
     2941 gaccgagaac aggagggcgg cgctgaccgc gtccggccac tgccagacga cgctcagcgg
     3001 cgtcaggacg atgaagatgc cgagcacgat gagctggaac cggaagcagc cgagacccgg
     3061 gccacgctca ggatcgaggt cgtcgccctt gtactggacc ggcttggcat tgatcgtcac
     3121 gagcgggaag ggtatgcgat gaccgcgcct gccgccgttc gtgccccgca acgcgtcggc
     3181 tgaccggggg ctatcctgac gatcctcgat ccagcgccat cacgcgcctc agactcgatc
     3241 gagcggaggg gccgccgtgt cccgacaacg ctggctcatc attgccctgc ccgcgctcgt
     3301 ggtcgggatc atcgaggcgc tcagcgacac cattctcgac ccagtcctgc ccttcccggg
     3361 tgacgcgctg atcgtgaccc tggccgtgct ccttctatcg atcgcagttt ccggcctggc
     3421 gttcggcagg atcgacgcgc tcggggccgc gctcgctgcg cggaaccggg aactcgagat
     3481 gcgagcgggc tcggcggcgg cgatgcacaa ggtcagcgtc gcgatcacgg cactggtgga
     3541 tctgcgctcg atcctcgaca cgatcgtgga caacgcccgg accctgctcg cggcggacgt
     3601 ggccgtgatc ctcctcgtcg atgccgacgg cgcgctgagg cgctccgcgt ccagcgggcc
     3661 ggtccgggag accggtgacc tcgccgagcg gtcgggcgcg gatccggacg acctcctcca
     3721 attcctgccg gccgacctgg cgaccagtcg actcgccaac gccctgaacc gcgggccgga
     3781 aacgatcggc ctgctggcga tcggctcgaa aagggcgagg tccttcgacg cggaagaggt
     3841 cgagacgctg gcctcgctcg ccaaccaggc ctcgatcgcg atcgagaacg ctcgcctcca
     3901 ggcgcgactg cgggagctcg cggtcgagac cgagcgggga cggatcgccc gcgagatgca
     3961 cgacgggctg gcccaggtgc tcggctacgt gagcacgaag tcacaggccg cggatgagct
     4021 gctggcggtc ggccgcgtcg ccgacgcccg cgctcagctc ggcgagctga cggcggccgc
     4081 gcgatcgatc tacgtcgacg tgcgcgaggc catcctcggc ctgcgcagcc cgatcacgcc
     4141 ggagctcggt ctcgtcggcg cgatcgagga ctacgcccgg cgcttcgccg atgcatccaa
     4201 gctggccgtc accgtggagg cctcgtcgga ggcccgcgca gccacgatgg gccccgaggc
     4261 cgaggcgcag gttttccgga tcgtccagga gagcctgacg aatgtccgca agcatgccgc
     4321 ggcgggtcgg gtcgcggtcc atctcggccg ggtcgacgac gacctcctcg tcgagctcga
     4381 cgacgacggc cgagggttcg atgagccgag cccgtggcag tcgaccgatt ggccgcacta
     4441 cggccaggcg gcgatgcagg agcgcgcggc cgacatcggg gcgcggatct cgtggggcac
     4501 gcgtccgaca ggcggcggct tcatccgact cgtggtgccg atcaacggct tcatcctcga
     4561 ggtcgcgacc tgatgcgggt cctgttggcc gacgatcacg ccctcgtccg cgacgggatc
     4621 gcgtcgctcc tttcggcatg gggacacgat gtcgtcggcc aggccgcgaa cggcgaggac
     4681 gcggtcgcgc tggcagatcg gctggaaccg gacctcatcc tgatggacgt ccggatgcct
     4741 ggctcgtccg gcctggaggc gactcgccgg atcgccggcc ggcatccggc catcgccatc
     4801 gtcatgctga ccgtctcgga ggacgaggac gatctgttcg aggcgatcaa ggccggcgcc
     4861 cgcggctacc tcctgaagaa tctcgaggcc cgcgagcttc gcgccatgct cgaggcgatc
     4921 gcccggggcg acgcggcgat ctcgccggcg acggcgttcc ggatcatccg cgagttcgcc
     4981 cgcgggccgg tgaccgacgc cgtcgacacg ccgaccgccc tcacggcccg tgagctggac
     5041 gtgctcggtc tcgtgacggc tggcctccgc aacaaggaga tcgctgcccg cctggggatc
     5101 agcgagaaca ccgccaagtt ccacctccgg aacatcctcg gcaagctcca cgcggagagc
     5161 cgcacggagc tcgcgacacg cgccctccgc gagaggctcg ttcccgacga ctgagcccgc
     5221 tcggggccga acggcccggc tgtccccagc caaaaggatg ggaccggccc gctcccgatg
     5281 agggtggcgt cgcgcctccg ctggtccgat gctcggaatg ccgaggacgg cgaaggaggc
     5341 gatcgatggg ctaccgaatc gatatcgacc acggcggctg catcaattgc ggcatctgca
     5401 tggacgtctg cccggtcgag gcgctcgaca tgagccgtcc ggagcggccc gggatcgagt
     5461 ctggccaggg attcggacag ccgttcgtct ggtcgatgga acaccccgtc caggtcggcg
     5521 aatgcatcgg ctgcaccgtc tgcatcgagc agtgccccgt caccgtgatg accctcgcca
     5581 cccagcccgg tccgaccgat ctcgctccac ggcaggggcc gatccatcga ccgctcccgg
     5641 agagcggcgg ctgggttccg ctctcggcgg tcaccctgga gtcgctcaaa ccggcccatc
     5701 cgtcgccgtg gagcgacgtg atcggcgtcc ggacgccctc gcgctggccg gccggcagcc
     5761 cactcccgca cgacaccgca cctcatcagg ctccatgtca ggtggcctgc ccggccggca
     5821 cagacgccgg acgctatgtc gggctggtcg cccagggccg ctacgacgat gcctatgcgg
     5881 tcgccgccga agtcaacccg ttcccatcgg tctgtggctg gatctgcacg gcgccatgcg
     5941 aggccgcctg tcggcggggc gtgctcgacg agccgatctc catccggacg atcaagcggt
     6001 tcgccgctga acatgggtcg ctgccggcgg tcaagccgcc cacgacgcgg cgcccggagc
     6061 gggtcgcgat cgtcggcggt ggtccggccg gaatggcggc cgcctggtat ctcgctcgcc
     6121 tcggatacgg ggttaccgtc ctcgaggcga tgcccgtccc gggcgggatg atggcgatcg
     6181 ggatccccgc ctaccgactg ccgcgggacg ttctccaggc ggagatcgcg cggatcgtca
     6241 gtgtcggcgt ggagctgcgg ctgaacgcgg tcatgggccg cgacttcacc ctgggcgacc
     6301 tggagcgcga gggctatcga gcggtcttcc tcgcgaccgg cgcgtcgaag agccggcgtc
     6361 tcgggatccc cggcgatgac ctcaccggag tcgtcccggc cacgttgttc ctgaagcgcg
     6421 tcaacctcgg cgaggagccg acgctcagcg gcgatgtcgt ggttgtcggc ggcgggagca
     6481 cggcgatgga cgccgcccgt tccgcgctgc gttccggggc cgcatcggtg accgtggcct
     6541 atcgccggac gagggccgac atgccggccc aggtcgagga gatcgaagcc gccgaacgcg
     6601 aaggcatcac catccggacc ggcctgaccc cggccgaggt cgtcggccgg gacggccagg
     6661 tcgtggccct tcgctgccgc ccgacggccg cgggcggcga gcaggccccg ttggatggcc
     6721 aggttaccgt cgattccgag gctgtcgctg tcgacctgcc cgggcgaacg atcctcgtcg
     6781 cgatcggcga ggagcccgat ccatcgatcc tgcccgaagg gagcgggatc gaggtcagtg
     6841 cctgggcggg cgtcgtcgca gacagtcgga cgctggccac cggccgcgcc gggatcttcg
     6901 ccggcggcga cgtcgtctcc ggcgcgaaga cgatcatcga cgcggtggcc gccggccgcc
     6961 gtgccgcggg cagcatccat gaatacctcg ccggggtcca cgacggcgaa gccgacattc
     7021 tccggacagt ccgctacgcg acggcgcccg agcacgatct cgtgctcgat ctcgagccgc
     7081 gtccgaggtt gcacgctccg ctgcccgtgg tcgacacgcg atccttcgcg ccggctcacc
     7141 cggggttcga ccccgccatg gcccgagccg aggcgggccg ctgcttccga tgcgatgcgg
     7201 tcgaagtctg caccacggtc catgtgaccg gcggtcgcgg acccgccgac cgaccggggc
     7261 tcactcccgg cagacaaccg accaccccga gccaggccca gcaaccgaca acgacaggag
     7321 gtgtggcgtg acgcccgacc aggtatccgg cttcttcgat gccggcgaga acttcgtcga
     7381 agggacgatc gccgcagcgc tcgtcgtgct ctggatgctg gcggtcgcgc ttcatctcgc
     7441 ccggccgtac atggtccgga acaccggcaa gttcagcctc cgcctcggcg ccgacctgtg
     7501 gtggatcatc tacgtcgggc tgcgcgacat catcctcgtc caggtcctgc tcggcagctt
     7561 catcttcttc tacccggatg tcgtcgccgg ccaggacctg ccgatcaccg gcggcctcgc
     7621 cgcggtctgc gccttcgccg cgctcctgat caagctcacg accgcaggcg atcgcgacat
     7681 caacggctat cgctggcagg tgatcctgat cggggtcggg gcagcgctgt acatcggccc
     7741 gtacttcctc ggaacccaga tgaccgagct caccggacag cgggtcgaca cgatcatgcc
     7801 gttcctcgtc agctcgaaga acctcgacct ggcgctgccg ctctgttacc tgtcgggcgc
     7861 gctggccggc gcgtgcggcg tgatcgccgt cctctacaac ctccgcggca ccggccggcg
     7921 gccggctgcg atgacgacga ccgaggagaa cgcacgatga atgccgacga tctggccact
     7981 gcgctgacga ctggtgcctc gggctggctc gtcctgtccg tcgcggcagt cctgccgatc
     8041 ctgtgggcgt tcaccctcgt gctccacttc gcacggccgt acgtgatccg gttcctgggc
     8101 gggctcaccc tccgcttcgg cggcgacgtg tggtggctgt cctacgtcct gttccgggac
     8161 gcgatcctcg tgatcacgct cggtctgagc atgatcttcc tcatgcccaa cctgtacctg
     8221 aaagtcggcc tgccgatcac ggcgccgatc gcgaccgtcg tcctgttctg ggcgttgctc
     8281 gtcaagctcg tccgcgacac ggacgacgat ccgggcgcgt tccggatctt cagcgtcctg
     8341 ctcgtgatcg cctcgatcct gtacatcgtg ccgcaggtct acggcctcga agccgccgac
     8401 caggagtatc tcgggagcct tcccgcgacc ctgatcagca gtgagaacct cgacctggcc
     8461 cggccgatcc tgtggatcag cctggcgctg ttcgccgcga ccggggcggg cctgttcacc
     8521 cggttcctcg tcatgctggg ccgggcggat ggccccgccg aggaggccgc ccagcccggc
     8581 tcgacggccg gctgaccagg aggatcgacg accgtcgcct cgctcgacgg gtgcgtgtgg
     8641 cgcaacggcc gcggagatcc tggcgtcagg cgccgggttc ggtctccggc acgtgcgcca
     8701 atccgctgcc acggcgggcg agccggtcct gcagccggaa ccattcgccc gcgaacggca
     8761 ggaaccacgg atggccttcg tacggcgccg gcaccagcgg gaacttcagc ttcgagaggg
     8821 ccggcgccgg gccgccgccc agccattcgc cgaccgccgt cccgagggcc gtcatcaggg
     8881 ccacgccggt cccgcagcag cccatcgcgt aggtgatccc gtccttccgc ccgacgtgcg
     8941 gcatccggtc gaaggtgaag ccgacgttcc cgccccaggc gtactcgacc cggtagccgg
     9001 cgagctgcgg atggacctcg agcatgccct tgtggaggat ccctgccgtc cggtcgaccg
     9061 aggtcggcag gaagctcgcc cggcctccga agatcatccg gcggtcctcc gacacgtgcc
     9121 agtagtagag gaagttcttc gtgtcgaaga acgcccggcc cttcggcgag agggtccgtg
     9181 ccagctcctc ggggagtggc tcggtggcga tgatgtagct cccgatcggg atcacccggc
     9241 ggcgcaggtc ggggacgacc ccgtcggtgt agccgttcgt cccgacgatg acctcattcg
     9301 tcaggatcgc gccgcgatcg gtctcgacca cgagccggcc gtccgcctgg cgacggatcg
     9361 tccgggcccg gacgccctcg tggagatcgg cgccggccgc atcggccgcg gccgcgaggc
     9421 ccgcgaagta cttgccggga tggagcaggc cgctcggcgg gaagaccagg ccgccgtggt
     9481 agaacgtcgt cccgatctcc tcgtggaggc gctccttcgg gatgtattcg gcctcgacgc
     9541 cgaaccgcgc cagcgtcgcg accgagtcgc ggagatcgtc cgcgtgggac ggcgcccagg
     9601 cgaggtcgag gaagccgcgc tcgcggaact cgcaatcgat ctgctcgtcg gcgatcaggc
     9661 gcttgacgag ctcgtagccg gccatcgtct cgccgaagag cgcccggccc gtttcgtcgc
     9721 cgtagcgctc gatcagggcc tgcggacccc atttgaaccc gggatgaacg atcccgccgt
     9781 tgcgggtcga gccgccgaac cccagatgct cggcctcgag gagggtgacc gcggcgcccc
     9841 ctcgggcgag gacccgagcg gcgttgatcc ccacgtaccc gccgccgatg acgacgacat
     9901 ccgcgctgtc gggaagttgg cgatcgcgac gcgacggcag gaccggcatc gtgtcgtgcc
     9961 aatacggccg ccgctcgtag tcgcggttca gggccgatcc tcgtcgggcg ctcgtaaggg
    10021 ccatcgccgg gagcctagcc cagcgcggac cgcgacgcgt ctccgttgcg ccggacggat
    10081 gatggtccgc agcgccccgg tgaaccgatg tcgggcgagc ggcatcgaat gggtgaacgc
    10141 gtcaggaggg acccgcgcat cggacccgcg atcgaccggt cgctgatcga ccgggcccgg
    10201 aacggcgatc tcgacgcgtt cgagtcgatc gtcaacgccc ggatggacgc cgtgtaccga
    10261 ctgaccagcg cgatcctcgg caacgaggcc gacgcccgcg acgccgccca ggagacgttc
    10321 gtcgccgcgt ggcgtcagct ggcgcgcctc cgcgagcccg agaagttcga ggcctggctc
    10381 cagcggatcg cgatcaacgc cgcacgccag acgatgcgtg cgcgcagccg gcgcaggatc
    10441 cgcgagatcc cgtcatcgac ggtcgtcgcg ctggcggagc gggtggcggg cggcccgcgc
    10501 gacgacgcgg ccatcctcga cgcggccctc gcgaccatgc cgatcgatca gcgggcgatc
    10561 ctcgtcctcc accacctgga gggccggtcg atcacggagc tggcggcgat cctcgacatt
    10621 ccggaaggca cggccaagtc gcgcctgttc gccgcgcggc gggcgctcgc ggcggccatc
    10681 acggcaagtg aggcaggcgg ccgatgacgt cgctcctgac ggccgactgg gacgacgagc
    10741 ggctcgacgc cgccttcgcg gcccgcgccg acgcggcgcc ggcgacgccg ccggacctcg
    10801 cagcggtcgt cgagcggctc gcccgccagc cgcgcccagc gcggggg
//
LOCUS       DATAIW010000093         7362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_240240, whole genome shotgun sequence.
ACCESSION   DATAIW010000093 DATAIW010000000
VERSION     DATAIW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240240"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VHM48_03140"
     CDS             <1..901
                     /locus_tag="VHM48_03140"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX2754429.1"
                     /translation="GLPVADLVNQGDFGRVMAEFWADGPKSETPPGHWNVLANLASDE
                     LAPDLHIAGAGPTIGRLEWDVKLYLALNGAVHDAAIAAWGLKGHYDSIRPISMIRYMG
                     GLGQSSDPKGPAYDKAGLPLVPGLIEVITRKSSAAGQRHAALAADVGRIAIKAWGGNP
                     ADPKTQASGAVWMLAGDWVPYQMPTFVTPAFAAYASGHSSFSRAAAEVMTAFTGSEYF
                     PGGLSGYTIKADSLKFEKGPTTDIQLEWATYYDAADQAGQSRLWGGIHIQADDFNGRI
                     IGSRCGKEAWMLAQRYYAGQVTP"
     gene            969..2639
                     /locus_tag="VHM48_03145"
     CDS             969..2639
                     /locus_tag="VHM48_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX2754430.1"
                     /translation="MTRRRNVAIVAALAVVGAVGVAASIRVLRPGATSAALGAPHFVD
                     ERATAGLVATYDGDSDFATGGGVAAFDCDDDGRSDLLVAGGAGPSTIDRNESPVGGPL
                     RFAPAASGTAGSQDAVLGPGVLGAYPLDVDGDGHVDLAILRTDGLRLLRGIGDCRFAD
                     GNPAWSLDGGSGWTSGFSASWEGTATLPTLAIGHYLKVVAGTPTFDCDDDALVRPLVE
                     GSAYGAPLTLAPGYCTLSMLFSDWDRSGRRDLRVTNDRQYYRDGEEQLWRVEPGATPR
                     LYTDADGWRRVEIWGMGIASQDVTGDGYPDVYLTSQGDNKLQTLAAGAAQPGYRDIAL
                     KRGVTVTQPFTGGDALPSTAWHPEFEDVNNDGFIDLFVSKGNVNVMPDYAAKDPSNLL
                     LGQPDGTFVEGAGAAGIVTFDRGRGAAIADFNLDGLLDLVQVNYGQPLRVWRNVGGGD
                     ATTPTGLGSWLALRLSQPGPNRDAIGAWVEVRVGDTTTTRELTVGGGHLGGKLGWVHF
                     GIGTADGADVRVRWPDGETGPWLHAAADGFFDIERGATAIQPWTPAHP"
     gene            2656..3573
                     /locus_tag="VHM48_03150"
     CDS             2656..3573
                     /locus_tag="VHM48_03150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX2754431.1"
                     /translation="MTARIPRLAEIGLPPDFGAPDLCPEPPRERYAERIEQLRARMTG
                     RTRTSGGIRRLLGGLRPGMTEREAVRLLDWDGTPLSCHLMLSSGPRATLGLLSPGDRV
                     IERGDRFTVAFGIWGALNCRAGFVVDNAEGLPAGIRDYVERLVGPYFAAVAEWYETIR
                     IGIPGGALQTVVDRHLGDPFFGTFLNPGHQIHLDEWVNSPISPGSTIPLRSGMAFQVD
                     IIPATGGPYFTTNIEDGIALADESLREAFAASYPGAWERIEARRRFMAEELGIRLHPD
                     VLPFSNLPAYLPPFLLRPDRAMTMVEAGS"
     gene            3570..4469
                     /locus_tag="VHM48_03155"
     CDS             3570..4469
                     /locus_tag="VHM48_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754432.1"
                     /translation="MSGSAGDIVRLADRAIEIEVLPAAGARLHRLRVFGHDLLRTPDD
                     LQRHVDDPYFWGSYPMAPWCNRVPSGPALAAGRAIDLPASFPDGTAIHGQVARRPWQV
                     AGHGRFQIDGGGDGWPWSYGVEQEIRIGDGVLLLRLRLTNRSDEPMPGGIGFHPWFRR
                     PIRVAIAAATVHPVNVATEALPVPVSGRYDRRRLEPMPDGLDSAWADLSDPPVVLDWP
                     DAGVRATMSIDAPNRFIVAASPIAIDAVAVEPETQAPDGLRRLARGEPGGLVLLPPGG
                     SLELVVRLAFETVSDRPDPARQR"
     gene            4505..4963
                     /locus_tag="VHM48_03160"
     CDS             4505..4963
                     /locus_tag="VHM48_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX2754433.1"
                     /translation="MNRPTLLTTIAAGRDRLDATLASLSDAAMLDRIDEAWTRKDILA
                     HLAAWERRVIEHFETLRAGDAPDGSIETDDLNNRFFVLDRDRPLEDVRAGERDAFRAV
                     LGAIERASDEELFDGQHFGWTEGDPLADWFRGNTDEHYDEHLEQLTRSAR"
     gene            complement(5179..6282)
                     /locus_tag="VHM48_03165"
     CDS             complement(5179..6282)
                     /locus_tag="VHM48_03165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX2754434.1"
                     /translation="MTIVEGTVDLARIVGVSEFEAFARRTMDPAAFDYVAGGAWDEQS
                     LGEAEAAWRKRRLRPRVLVDVSRVDPSTTMAARPVALPLAIAPMAAHGLAHPDAEIAT
                     ARAAAAAGIPFTLSTMSTNSIEEVAAAAPDAVRWFQLYPQAEPRRSRSFVERAAAAGY
                     SAIVVTVDLPVLGYRERDLRSGFNLAVPHGNFDGGAGPDHASHGGARDSGYDILADQL
                     DQGLAWSGLAAIQSWSSLPIFLKGILTAEDARIAVEHGVAGIIVSNHGARQLDRTVAT
                     ADALEEVVAAVGGRAEVWVDGGIRRGLDVVTAVALGARGVLVGRPILWALAAGGQAGV
                     ERALAILRTETILAMTLLGAPTPADLTRAHVAG"
     gene            complement(6279..6686)
                     /locus_tag="VHM48_03170"
     CDS             complement(6279..6686)
                     /locus_tag="VHM48_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754435.1"
                     /translation="MTTSLRVGLETLSIASVVLAGWLASVVLNVLPTRDPGHVQLWSL
                     VTVGVVCFAAASFLAIRPVRRAPPFPAGLAAALGVLAVAAVGFGLFVLGSEMSDALAG
                     DPEGYLFVVGVIVTAHGALALGWLAAVRMGLRR"
     gene            complement(6696..>7362)
                     /locus_tag="VHM48_03175"
     CDS             complement(6696..>7362)
                     /locus_tag="VHM48_03175"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX2754436.1"
                     /translation="DDEKELEEERRLAYVGMTRAMRRLFLSHAWRRATWGMGQPSVPS
                     RFLLEIPAELMIGPDLASGSAGLDVDMDLAFGVRGTTRFGRPIRNGLGGGAAYRQGSG
                     TPGAPPPGQAFRPTRDLTARREAYDEGAPSGSLARPGATIPGAASPAARAEPARPARP
                     IIPGERQYRDGDRIRHGRWGDGIVVTSKLTRSDEEITVAFKDPAVGRKTMLASLANLE
                     IIG"
BASE COUNT         1066 a         2700 c         2578 g         1018 t
ORIGIN      
        1 gggcttgccg gtcgccgacc tggtcaacca gggtgacttc ggccgggtca tggccgagtt
       61 ctgggccgac ggcccgaagt ccgagacgcc acccggccac tggaatgtcc tcgccaacct
      121 cgcctcggac gagcttgccc ccgacctcca catcgcgggc gccggaccga ccatcggccg
      181 gctggagtgg gacgtgaagc tgtatctcgc cctgaacggc gccgtccacg acgcggccat
      241 cgccgcctgg ggactcaagg gccattatga ctcgatccgg ccgatctcga tgatccgcta
      301 catgggcggg ctgggccagt cgagcgaccc gaaggggccc gcgtacgaca aggcgggcct
      361 gccgctcgtg ccgggcctca tcgaggtcat caccaggaag tcgagtgcgg ccggccagcg
      421 ccatgccgcg ctggcagcag acgtcggcag gatcgcgatc aaggcctggg gcggcaaccc
      481 ggccgacccg aagacccagg cgtccggcgc ggtctggatg ctggccggcg actgggtccc
      541 ctaccagatg ccgacgttcg tgactccggc gttcgcggcg tatgcctccg gccacagctc
      601 gttcagccgg gcagccgccg aggtcatgac cgccttcacc ggcagcgagt acttccccgg
      661 cgggctgagt ggctacacga tcaaggccga ctcgctcaag ttcgagaagg gaccgacgac
      721 cgacatccag ctcgaatggg cgacgtacta cgacgccgcc gaccaggccg gccagtcgcg
      781 gttgtggggc gggatccaca ttcaggccga cgacttcaac ggccggatca tcggctcacg
      841 ctgcggcaag gaggcatgga tgctggccca gcggtactac gccggccagg tcacgccgtg
      901 agacggccgc cgggtcctcg ccgcccccct gatccggacg accgccgccc gggaggatcg
      961 gggctgccat gacacgacgg cgaaacgtcg cgatcgtcgc ggcgctcgcc gtggtcggcg
     1021 cggtcggggt cgccgcttcg attcgggtcc tgcgccctgg agcgacctcg gccgcgctcg
     1081 gcgcgccgca tttcgtcgac gagcgggcga cggccgggct cgtcgcgacc tacgacggtg
     1141 actcggactt cgcgactggg ggcggggtcg ctgccttcga ctgcgacgac gacggacggt
     1201 cggacctgct cgtcgccggc ggtgccggcc cgtcgacgat cgatcgcaac gagagcccgg
     1261 tgggtggtcc cctgcgtttc gctcccgccg cctccggcac cgccggctcg caggacgcag
     1321 tgctcgggcc gggcgtgctt ggcgcgtatc cactcgacgt cgacggggat ggccacgtcg
     1381 acctggcgat tctccggacc gacggcctgc ggctgctccg cgggatcggg gattgccggt
     1441 tcgccgacgg caacccggca tggtcgctcg acggcgggtc aggctggacg tccgggttca
     1501 gcgcgtcatg ggaggggacg gcgaccctgc cgaccctcgc gatcggccac tacctgaagg
     1561 tcgtcgccgg cacaccgacc ttcgactgcg acgacgacgc gctcgtccgg ccgctggtcg
     1621 agggctcggc ctatggcgct cccctgaccc tcgcgccggg ctattgcacg ctgtcgatgc
     1681 tgttcagcga ctgggatcgt tcgggacggc gcgaccttcg agtgaccaac gaccgccagt
     1741 actaccgcga cggcgaggaa cagctgtggc gggtcgagcc gggcgccacg ccgcgcctct
     1801 ataccgacgc cgacggctgg cgccgcgtcg agatctgggg catgggcatc gccagccagg
     1861 acgtgaccgg cgatgggtat ccggacgtgt acctgaccag ccagggcgac aacaagctcc
     1921 agaccctcgc tgccggcgcg gcccagccgg gctatcgcga catcgccctg aagcgcgggg
     1981 tgaccgtcac ccagccgttc accggcggcg acgcgttgcc ctcgaccgcc tggcatcccg
     2041 agttcgagga cgtcaacaac gacgggttca tcgatctctt cgtctccaag ggcaacgtca
     2101 acgtgatgcc ggactacgcg gcgaaggacc cgagcaacct cctcctcggc cagcccgacg
     2161 gcacgttcgt cgagggcgcc ggcgccgccg gcatcgtcac cttcgaccgc ggccgcgggg
     2221 cggcgattgc cgacttcaac ctcgacggcc tgctcgacct cgtccaggtc aactacggcc
     2281 agccgctccg ggtgtggcgg aacgttggcg gcggcgacgc gacaacgccg accgggctgg
     2341 gctcgtggct cgccctgagg ctgtcccagc ccggtccgaa ccgcgacgcg atcggcgcct
     2401 gggtcgaggt ccgcgtcggc gacacgacga cgacccgcga gctgaccgtc ggtggaggtc
     2461 atctcggtgg caagctcggc tgggtccatt tcggcatcgg gacggccgac ggagccgacg
     2521 tccgcgtccg ctggcccgac ggagagaccg gcccgtggct ccatgccgcg gcggacgggt
     2581 tcttcgacat cgaacggggc gcgactgcca tccagccatg gacgccggcc catccgtgat
     2641 ggaggatcga gcacgatgac cgcccggatt ccgcgcctcg ccgagatcgg gctgccaccc
     2701 gacttcggcg caccggacct gtgccccgag ccgccccgcg agcgatacgc ggagcggatc
     2761 gagcagctcc gcgcccggat gacgggccgc acccggacgt ccggcggcat ccgccgcctg
     2821 ctcggcggac tccggccggg gatgaccgag cgcgaggccg tccgcctgct cgactgggac
     2881 ggcacgccgc tgtcgtgcca cctgatgctc tcgagcgggc cccgggccac gctcgggctg
     2941 ctcagccccg gcgatcgcgt catcgagcgc ggcgaccgat tcaccgtcgc gttcgggatc
     3001 tggggcgccc tcaactgccg ggccgggttc gtcgtcgaca atgccgaggg gctgccggca
     3061 gggatccgcg actacgtcga gcgcctcgtc gggccgtact tcgccgccgt cgccgagtgg
     3121 tacgagacga tccggatcgg gatcccgggc ggcgccctcc agacggtcgt cgaccgccat
     3181 ctcggcgatc cgttcttcgg gaccttcctg aacccgggcc accagatcca cctcgacgaa
     3241 tgggtcaact cgccgatctc gcccggctcg acgatcccgc tgcggtcggg catggcgttc
     3301 caggtcgaca tcattccggc gaccggcggg ccgtacttca cgacgaacat cgaggacggg
     3361 atcgccctcg ccgacgaatc cctgcgggag gccttcgcgg cgtcgtatcc gggcgcctgg
     3421 gagcggatcg aggcacgccg gcggttcatg gccgaggagc tcggcatccg gctccatccc
     3481 gacgtgctcc ccttctccaa cctgccagcc tacctgccgc cgttcctcct ccgacccgac
     3541 cgggcgatga cgatggtcga agcgggctca tgagcggatc cgccggcgac atcgtccggc
     3601 tcgccgaccg cgcgatcgag atcgaggtcc tgcctgccgc cggtgcgcgc ctccaccggt
     3661 tgcgcgtctt cggccacgat ctgctgcgga cacccgacga tctgcagcgc cacgtcgacg
     3721 atccgtactt ctggggcagc tacccgatgg cgccgtggtg caaccgggtg ccgagcggac
     3781 ccgccctcgc cgccgggcgg gcgatcgacc tgccggcgag cttcccggac gggaccgcga
     3841 tccatggcca ggtcgcccgg cgaccgtggc aggtcgcggg gcacggccgg ttccagatcg
     3901 acggtggcgg agatggctgg ccgtggtcgt acggcgtcga gcaggagatc cggatcggcg
     3961 acggcgtgct cctgctccgg ctgcgcctga cgaatcggtc cgacgaaccg atgcccggtg
     4021 gcatcgggtt ccatccgtgg ttccggcgtc cgatccgggt cgcgatcgcc gccgcgacgg
     4081 tccacccggt caacgtcgcg accgaagcgc tgccggtccc ggtcagcggc cgatacgacc
     4141 ggcgccgact cgagccgatg ccggacggcc tcgattcggc gtgggccgac ctgagcgacc
     4201 cgccggtcgt cctcgactgg cccgacgccg gggtccgggc gacgatgtcg atcgatgccc
     4261 cgaaccggtt catcgtcgcg gccagcccga tcgccatcga tgccgtggcc gtcgaacccg
     4321 agacccaggc ccccgacggg ttgcgccggc tcgctcgcgg cgagccgggc ggtctcgtcc
     4381 tcctgccgcc cggtggatcg ctggagctgg tcgtccggct cgccttcgaa accgtcagcg
     4441 accgtccgga tccggcgcgc caaaggtgac aggacctgac gcagcccggg cccacgatga
     4501 cgacatgaac cgaccgaccc tcctcaccac gatcgcggcc ggtcgcgatc gcctcgacgc
     4561 caccctcgcg agcctgtccg atgcggcgat gctcgaccgg atcgacgagg cgtggacccg
     4621 caaggacatc ctcgctcacc tcgccgcatg ggagcgccgc gtcatcgagc atttcgagac
     4681 cctccgggcg ggggacgccc ccgacggatc gatcgagacc gacgacctca acaaccgctt
     4741 cttcgtcctc gaccgcgacc gcccgctcga ggacgtgcgc gccggggaac gcgatgcctt
     4801 ccgggcggtc ctcggcgcga tcgagcgggc gtccgacgag gagctgttcg acgggcagca
     4861 tttcgggtgg accgagggcg acccgctcgc cgactggttc cgcggcaata ccgacgagca
     4921 ctacgacgag cacctggagc agctgacccg ctcagcccgc tgagcgctca ttgggtcgcg
     4981 cgcccggcga ggccgacccg gttcccaccg ggccatctcg tctagcgcac acctcggcgc
     5041 cttgaggcgg gtatcgccgc ctgctccggt cgattcattg cgctcgatgc acgaattcaa
     5101 ccagacgcgg ctgtcaacgg ctcctttcag tcgagttcgt tgcacccgga cgcaacggaa
     5161 ccggttgcct tcggcgcctc agcccgcgac gtgggcccgg gtcaggtcgg ccggcgtcgg
     5221 cgccccgagc agggtcatcg ccaggattgt ctcggtccgg aggatcgcga gtgcccgttc
     5281 gaccccggcc tggccgcccg cggcgagggc ccacaggatc ggacggccga cgagcacgcc
     5341 gcgcgccccg agggcaacgg ccgtcacaac gtcaaggccg cgccggatcc cgccgtcgac
     5401 ccagacctcc gcccggccgc cgactgcggc gaccacctcc tcgagcgcgt cggcggtcgc
     5461 caccgtccga tcgagctggc gggcgccgtg gttcgacacg atgatccccg cgaccccgtg
     5521 ctcgaccgcg atccgggcgt cctccgcggt caggatgccc ttgaggaaga tcggcaacga
     5581 cgaccagctc tggatcgccg ccaggccgga ccaggcaaga ccctggtcga gttggtccgc
     5641 gaggatgtcg tagccgctgt cgcgagcgcc gccgtgggat gcatgatcgg gcccggcgcc
     5701 gccgtcgaag ttgccgtgtg ggaccgccag gttgaagccc gagcgaaggt cccgttcgcg
     5761 atagccgagg accggcaggt cgacggtgac cacgatcgcg ctgtagccgg ccgccgccgc
     5821 ccgctcgacg aagctccggc tccggcgcgg ctcggcctgg ggatagagct ggaaccagcg
     5881 aactgcatcc ggcgcggcag ccgcgacctc ctcgatcgag ttcgtcgaca tcgtcgagag
     5941 ggtgaacggg atcccggcgg ccgcggcggc gcgggccgtc gcgatctcgg cgtcgggatg
     6001 ggccaggccg tgggccgcca tcggggcgat ggcgagcggc agcgcgaccg gccgggccgc
     6061 catcgtcgtc gacggatcga cccggctgac gtcgacgagg acgcgtggtc gcagacggcg
     6121 tttccgccag gctgcctcgg cctccccgag cgactgctcg tcccaggccc cgccggcgac
     6181 gtagtcgaac gccgccggat ccatcgtgcg tcgggcaaac gcttcgaatt cagagacgcc
     6241 gacgatcctc gcgaggtcca ccgtcccttc gacgatcgtc atcggcggag gcccatccgg
     6301 accgcggcca gccatccgag cgccagggcg ccgtgggccg tgacgatcac cccgacgacg
     6361 aacaggtagc cctcgggatc gccggccagg gcgtcggaca tctccgaccc gaggacgaac
     6421 aggccgaaac cgacagcggc gactgccagc acgccgaggg cagccgccag cccggccggg
     6481 aacggcggcg cgcggcggac gggacggatc gccaggaacg acgcggcggc gaagcagacg
     6541 accccgaccg tgacgaggga ccacagctgg acatggccgg gatcacgtgt cggcagaacg
     6601 ttcaggacga ccgacgcgag ccaacccgcc agcaccacgc tggcgatcga cagcgtctcg
     6661 aggcccactc ggaggctcgt cgtcatggac cgagcctagc cgatgatctc caggttcgcg
     6721 aggcttgcca gcatcgtctt tcgcccgaca gccgggtcct tgaatgcgac ggtgatctcc
     6781 tcgtcgcttc gcgtcagctt cgaggtgacg acgatcccgt cgccccagcg gccgtggcgg
     6841 atccgatcac cgtcccgata ctgccgctcg ccggggatga tcgggcgagc tgggcgagca
     6901 ggttcggcgc gcgcggccgg gctcgctgcg ccggggatcg tcgcgccggg gcgtgccagc
     6961 gagcccgatg gcgcgccctc gtcgtacgcc tcgcgcctgg cggtcaggtc acgggtcggg
     7021 cggaacgcct ggccgggcgg cggggcgccc ggggtgccgc tcccctggcg gtaggcggct
     7081 ccgccaccga gtccgtttcg gatcggccgg ccgaaccgcg tcgtgccgcg cacgccgaag
     7141 gcgagatcca tgtcgacatc caggccggcg ctacccgacg cgagatccgg cccgatcatc
     7201 agctcggccg ggatctcgag gaggaaccgc gacggaaccg acggctggcc catcccccag
     7261 gtcgcccgtc gccaggcgtg ggacaggaac aggcggcgca tcgcccgggt catcccgaca
     7321 taggcgaggc ggcgctcctc ctcgagctcc ttctcgtcgt cg
//
LOCUS       DATAIW010000094         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_210401, whole genome shotgun sequence.
ACCESSION   DATAIW010000094 DATAIW010000000
VERSION     DATAIW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210401"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHM48_03180"
     CDS             <1..538
                     /locus_tag="VHM48_03180"
                     /inference="COORDINATES: protein motif:HMM:NF021610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HEX2754437.1"
                     /translation="PTGAQPGGPPPAPAEPRQRWRLTFARDAVAADQVGRAVLDAWQE
                     SLAASGLRLAGLEAGGAGRARIAFAAPLPAAARGEAELADLWLLERRPLWSVREALAD
                     RLPAAHRWIGAEDVWLGAPALAGQVVAADWRVELAGPGLVGDGARDRIAEAAMSCRGR
                     VSRARRRSATTCGPSSRR"
     gene            535..744
                     /locus_tag="VHM48_03185"
     CDS             535..744
                     /locus_tag="VHM48_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754438.1"
                     /translation="MTIEPAAGSSAEAPVVVRLRTRIHPELGAGRPEEVIAALAEVAG
                     IALEIAAIARVRLLLADDQPGRGRR"
     gene            complement(800..1177)
                     /locus_tag="VHM48_03190"
     CDS             complement(800..1177)
                     /locus_tag="VHM48_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754439.1"
                     /translation="MLGNHPVHPVLLSLDLMETRAFYHDKLGLEILKEDEHAIEFRCG
                     GTKLVVTKSTTGTADSQTQIGWDVPDLAAELAELRSRGVTIEEYDLPGLKTENGVADF
                     GFAWMAWIVDPGRNALSILELKG"
     gene            1379..2065
                     /gene="rplU"
                     /locus_tag="VHM48_03195"
     CDS             1379..2065
                     /gene="rplU"
                     /locus_tag="VHM48_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX2754440.1"
                     /translation="MYAVIETGGKQYRVEVGTELEVELLHAEPGDSIKLERILLVVDG
                     DESAIGHPIVADAAVEADVVRRDRGVKTVSFKYRPKARSRVKKGHRQELMILRITDVK
                     LGSKSAAADKQKADAAAKTERQRLEEAAAKQAAEDAALAAKLAKASAAKTPPKESATK
                     ADSKAAPKPKSTAKAATKPATKTSAKAPAAKADSPKTPRAKTQSGAADAKPKNAPAAK
                     PKPRATKKDE"
     gene            2071..2358
                     /gene="rpmA"
                     /locus_tag="VHM48_03200"
     CDS             2071..2358
                     /gene="rpmA"
                     /locus_tag="VHM48_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX2754441.1"
                     /translation="MAHKKAGSSSKNGRDSVGQRLGVKVGDGQLVPAGSIIVRQRGMT
                     FIAGTGTGLGRDYTVFATLTGKVKFEHATRNKKRIRVEAVEAPTAPAAAKA"
     gene            2433..2645
                     /gene="rpmE"
                     /locus_tag="VHM48_03205"
     CDS             2433..2645
                     /gene="rpmE"
                     /locus_tag="VHM48_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2754442.1"
                     /translation="MKPDIHPKYFEAKVHCGSCGTEWTVGSTRPELRVDVCSNCHPFF
                     TGKQTIIDTAGQVERFQKRLERSARA"
     gene            2935..3531
                     /locus_tag="VHM48_03210"
     CDS             2935..3531
                     /locus_tag="VHM48_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2754443.1"
                     /translation="MSERLVEQARRGDADAFVALIEERQVAMTRVATAILRDPADVAD
                     ALQETLVSLWRELPSLRSVDAFPAWADRVLVNACRLVLRRRGRRRLREIALPGVERAG
                     GGAMGPAAGRSAGFDTNVAERDAFDRAFETLDADARAILVLHHLEGQPIAAIAVALGI
                     PHGTAKSRLFTARRRLEEALAREERDESGRDLATESRR"
BASE COUNT          565 a         1243 c         1252 g          488 t
ORIGIN      
        1 cccgacgggc gctcagcccg gcggtccccc tccggcgccc gccgagccgc gccagcgctg
       61 gcggctgacc ttcgcccgtg atgcggtcgc cgcggaccag gtcggacgag ccgttctcga
      121 cgcgtggcag gagtcgctcg ccgcatccgg cctgcgcctt gccgggctcg aggccggtgg
      181 tgccggacgg gcccggatcg cattcgccgc accgcttccc gccgccgcgc gcggcgaagc
      241 cgagctcgcc gacctgtggc tgctcgagcg tcgaccgctg tggtccgtcc gcgaggccct
      301 ggccgatcga ttgccggctg cgcatcgctg gatcggcgcc gaggacgtct ggctcggagc
      361 accggccctt gccggccagg tcgtcgccgc cgattggcgg gtcgagctcg ccgggccggg
      421 gcttgtcggc gacggagcac gcgatcggat cgccgaggcc gcgatgagct gccgcggacg
      481 cgtctcaagg gctcgtcgga gaagcgctac gacctgcggc ccctcgtcgc ggaggtgacg
      541 atcgagcctg ccgccggatc atccgcggag gcgcccgtcg tcgtccgcct ccggacgcgg
      601 atccatcccg agctcggagc cggccggccc gaggaggtca ttgcggcgct ggcggaggtg
      661 gccggcatcg cgctggaaat cgccgcgatc gcccgggtcc ggctcctgct cgccgacgat
      721 cagcccgggc gcggtcgccg gtagggctcg ggcgctcgcg ccgccctcga ctacggcaac
      781 gagcgaccgc gggagctggt cagcccttca gttcgaggat cgagagggcg ttcctgccgg
      841 ggtcgacgat ccaggccatc cacgcgaagc cgaagtcggc gacgccgttc tccgtcttca
      901 gcccgggcag gtcgtattcc tcgatcgtga caccgcggga tcgcagctcg gcgagctccg
      961 cagcaaggtc gggaacgtcc caaccgatct gtgtctggct gtccgcggtc ccggtcgtgc
     1021 tcttcgtgac gacgagcttc gtcccgccgc agcggaactc gatcgcatgc tcgtcctcct
     1081 tgaggatctc gagaccgagc ttgtcgtggt agaaggcgcg ggtctccatg aggtccaacg
     1141 acagcaggac cggatggacg ggatggtttc cgagcatgca tgcctccctg tggcggacgg
     1201 cgaccggcgg cgcgcccaca tcgtcgtcga tacgcgacgg accgtgctcg acggtcaatt
     1261 gacgctcggg cgcctctcgc catacactcc gcgtccgcgc tcaggccacg gccggcgcct
     1321 ctccatgtcc gttgtcgcgg tcggaccacg accgccacgc cagtgttcga agggttccat
     1381 gtacgcagtc atcgagaccg gcgggaagca gtaccgcgtc gaggtcggca ccgagctcga
     1441 ggtcgagctg ctccatgccg agcccggcga cagcatcaag ctcgagcgaa tccttctcgt
     1501 cgtcgatggc gatgaatccg cgatcggcca tcccatcgtc gccgacgcgg cggtcgaggc
     1561 cgacgtcgtc cgtcgcgatc gcggcgtgaa gacggtctcg ttcaagtacc gcccgaaggc
     1621 ccggagccgg gtcaagaagg gtcatcgcca ggagctgatg atcctccgga tcacggacgt
     1681 caagctcggc agcaagagcg ccgccgccga caagcagaag gccgacgccg ccgccaagac
     1741 cgagcgccag cggctcgagg aggctgccgc caagcaggcc gccgaggacg ctgctctcgc
     1801 cgcgaagctc gcgaaggcct cggccgcgaa gacaccgcca aaggagtcgg ccacgaaggc
     1861 cgacagcaag gcggcgccga agccgaagtc gaccgccaag gcagccacca agcccgccac
     1921 caagacgagc gccaaggccc cggcggccaa ggccgactct ccgaagaccc cgcgcgccaa
     1981 gacgcaaagc ggggcggccg acgccaaacc gaagaatgca ccggccgcca aaccgaagcc
     2041 gcgcgcaacg aagaaggacg agtagattcg atggcccaca agaaagccgg ttccagctcg
     2101 aagaacgggc gcgactcggt cggccagcga ctgggcgtga aggtcggcga cggccagctc
     2161 gtccccgccg gctcgatcat cgttcgccag cggggcatga cgttcattgc gggcaccggg
     2221 accgggctcg gcagggatta caccgtgttc gcgaccctga ccggcaaggt caagttcgag
     2281 cacgccacca ggaacaagaa gcggatccgg gtcgaggccg tcgaggctcc gaccgcaccc
     2341 gccgctgcga aggcctgacg cccgaccagc agtagccggc gatcaccggc tgggccatcg
     2401 cccggcacgg acgacgtcgg aggagaacca tcgtgaagcc cgacatccat cccaagtact
     2461 tcgaggccaa ggtccactgc ggatcgtgcg gcaccgagtg gaccgtggga tccacccgac
     2521 ccgagctccg cgtcgacgtc tgctcgaact gccacccgtt cttcacgggc aagcagacga
     2581 tcatcgacac ggccggccag gtcgagcggt tccagaagcg cctggagcgc tccgcccgag
     2641 cgtaggttct cgttccttct tcgcaactgc cgaacggcgg gtctccggac ccgccgtttt
     2701 cgattcccga gatcgatcca cgcgatccac ggcgatcagg gtcaccgatc aaatcggggc
     2761 gcgatgagcg tcgcaccctc gccccggaac ccgttgagcg tcggtctggt gcacctgccg
     2821 cgctcattgg atccgattcc gatcagcctg agcgatcagg gtggccggga atgaaccgcg
     2881 gtgcgacgtc cgtactctga tggggtgagt ggcggcgaac ggcagggagg cggagtgtcg
     2941 gagcggctcg tcgagcaggc ccggcgcggg gatgccgatg ccttcgtcgc cctgatcgag
     3001 gagcggcagg tggcgatgac ccgggtggcg acggcgatcc tgcgtgatcc ggccgacgtc
     3061 gccgacgcgc tccaggagac gctcgtctcg ctctggcgcg agctgccctc gctgcgctcg
     3121 gtcgatgcct tcccggcgtg ggccgatcgg gtgctggtca acgcctgccg gctggttctt
     3181 cggcggcgcg gccggcggcg cctgcgcgag atcgcgctcc cgggcgtgga acgagccgga
     3241 ggcggggcca tggggcccgc tgccgggcgg tcggcgggat tcgacacgaa cgtggccgag
     3301 cgggacgcgt tcgatcgagc gttcgagacg ctcgatgctg atgcccgggc gatcctcgtg
     3361 ctccaccacc tcgagggcca gccgatcgcc gcgatcgccg tggccctcgg gatcccgcac
     3421 ggaacggcga agtcgaggct gttcaccgca cggcgccggc tcgaggaagc gctggcacgc
     3481 gaggagcggg atgagagcgg tcgtgacctg gcgacggaga gccgacgatg accccgctga
     3541 ccgacgag
//
LOCUS       DATAIW010000095         8231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_153407, whole genome shotgun sequence.
ACCESSION   DATAIW010000095 DATAIW010000000
VERSION     DATAIW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153407"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VHM48_03215"
     CDS             complement(<1..889)
                     /locus_tag="VHM48_03215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX2754444.1"
                     /translation="MRRFDVPDRLPAVGLFGGALVLAALAWIGLIGAGSGPIAPPVIA
                     VGVVVALGVVVGAGVVLLRRLRGSAATLERRTREVEEAGSRLRLLVEHVPAAVYIDMA
                     DPDVSDGGRLAYMSPQISGILGYRPDELVDDPELWPSRIHPDDREAAIAAYADHWRTG
                     QPLGAEYRMLARDGSEVWVRDEAYAMPDDTHSGRQVSQGLLIDTTDRKLLESKLIHDA
                     LHDPLTGLANRVLLRDHLERALARQVRNRGMVALLFADLDDFKRVNDSYGHAAGDQIL
                     TQVAQRLIGAVRADDVVARQ"
     gene            complement(969..1958)
                     /locus_tag="VHM48_03220"
     CDS             complement(969..1958)
                     /locus_tag="VHM48_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754445.1"
                     /translation="MSEQPESGSDSASTSETGASWSANGTQHEDAIAVAPETEVADVD
                     PTAEPAATSTSSDDAATFLSELVRAMQTTVGAERARIATETDRRREEHLAAIQTRRES
                     EAEKMRGLAADDLKAIDAWAEDERQRIQLEHERRAAALGEDLQKSLAEHGAKVDREGE
                     GVEAAIATYRVDVDAFFGALDRETDPVAIAQHASQRPVFPTLDQAGEADATPATSVGD
                     APSTDQDNDAPEPTGVGVMDPDSGSRLAASFAAWNASGSAAPLELSADVSADGSTDAG
                     SAADVAESGVLVAVAQGANDDGSESILHAVPSGRPLSWLRRDRDSSDRADEER"
     gene            2188..3180
                     /locus_tag="VHM48_03225"
     CDS             2188..3180
                     /locus_tag="VHM48_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754446.1"
                     /translation="MPRRIWLAAIVAAIASMGVALAPAAAADPGVANGRIAFGTRANG
                     SFNIETVMPDGTDLKAVTSGTGFHLCPDYTPDGRQIVFCANTSGAFELWTMKQNGGKP
                     TQLTHFGGFATFPDVSPDGSNIAFSGFLGDPANDQIFVVDAATGGGVVQLTTCPDAHP
                     WCFSSYPVWSPDGSKIAFSHADGFDADDTPENEQIYVMNADGSNPHAITTGPAPKDQV
                     PDWSPDGSKIVFTVGYFGSGGIWTINADGSGARQLTGCTASDPTPCPTGDDWGTAWSP
                     DGRKIVFLRDLTSLGIADRQVTIINADGTGAHSITTPGLHAVPAWQPRGVPSGG"
     gene            3255..3818
                     /locus_tag="VHM48_03230"
     CDS             3255..3818
                     /locus_tag="VHM48_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754447.1"
                     /translation="MGTENAEVVTRAMHGDHDAFAALIGLATNRLYALACLILRDPDR
                     AEDAAQEAIVRAWRELPRLRDPEKFDAWLRRLTVNACFDEARRIHRRAEVSLVRLGDR
                     ATADPAGAFVEHDRVDRAFRRLPFDQRTVLVLQHHFDLSHTEIAETLGIPLGTVKARI
                     RYGSAALRASLDADDRSSFAASDRTSA"
     gene            3902..5098
                     /locus_tag="VHM48_03235"
     CDS             3902..5098
                     /locus_tag="VHM48_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754448.1"
                     /translation="MAPPRAPERLLEEAFARTMTARQLRVYPWHVVRLGRRGGSSGNR
                     IGGIAFIGVAVAVAIAVAAGLVVRPGQNISGQPTPSLTPPSPSPSPSPPASAVPFPSA
                     VVVEPTIAIPVTGPITIATDGSSVWLFTATGDLVRIDPQTNAVAASVKLTPATDAYQS
                     LAGNQTGLWVTDWDTNQLLRFEPRTLRSVTTIDMGSQPKGVLVTGSAVWIANTRGGSV
                     QRIDPKTNKIAKTIPVGPTGPSGPNWLAQGSGSIWVGIPNSGSVVRISETTNAIEATI
                     AIPGPASPCGGLAAGTTAIWITSCDGGNLVTQIDPATNAVVGTIDLGGRGYTFAMIAD
                     RPWISPTDGQIVRVDPVRHVVDRVIAPGTGFSGGGDVVAAAGSLWVIDGAANRVLRLP
                     IAAFGG"
     gene            complement(5113..6924)
                     /locus_tag="VHM48_03240"
     CDS             complement(5113..6924)
                     /locus_tag="VHM48_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX2754449.1"
                     /translation="MRRLGPGAQVVAALAAAIVLVGGGLVPHVGAQGPDRSDVVLVLD
                     FSASILDDKTNRNRFGAALERIADRVDEISTDLVAGDTTVTIVQFATRAAGYPGCTDL
                     KLLDRAPTVARFADCLRSVAVAYRKGLTSALTGKIGVDTNYVAAMEAAARHLPADAVR
                     PAMILFTDGKHDVKGVPVSQVHVALERLFGSRSPFALLPVGMGLDPKKRSALTSGLEG
                     LRIIRDMPACISGATFDWPQVVFQTADQAGNAVAVALQDATCTFTAAPTPTPTPVPTP
                     AAVKGIRLTAGDGRIELTWTPAAATAAPIVDYAVRCRAGDGDWIESTEGVSLEPTATI
                     EGLTNGTAYQCEVAAVGPSAAGPWTASAGSVTPFGRPAAPAKPSVEALNHALRIAVTP
                     AGGQDVTSYHYECSSDQGGTWPATVDAAPDDTSGQIGNLTNGVDYTCRAFAKNALGQS
                     EASPLSDAVKPCGSTLECNSLLLPVLGGLGLLLAGGVLVALVALLRGRNLGHVVAVVD
                     VVHTANVGHGSHLGIAFVRAPDTRKVTGIVADRGSHADLRIRRLRGGRFEVRDKVGRH
                     VTADGEAVVVVDSVGVKHGLVLQAFATNAASRVASRR"
     gene            complement(7006..>8231)
                     /locus_tag="VHM48_03245"
     CDS             complement(7006..>8231)
                     /locus_tag="VHM48_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2754450.1"
                     /translation="VPNLDTGNTGFMLLCTSLVMLMTPGLAFFYGGLVSRRNVLGIMI
                     QSYVSMGWTTVLWVVVGYSLCFSGGQGGIIGDLNMAFLNGVTLDTPSTINDTIPAIVF
                     IAYQMMFAIITPALITGAFANRVTFKAYMLFLTGWLLFVYFPFVHMIWGGGFLAERGV
                     LDFAGGIVVHAIAGTAALASVLYVGRRHSPDGGAHSIPLVALGTGLLWFGWYGFNAGS
                     EFRVDSVTAVAFLNTDIAASFAAMVWLAIEWRHTGQPKFLGLLTGAVAGLATITPAAG
                     YVSPVTAVIIGCLAGVVCYLAVALKNRLRLDDALDVWGVHGVGGSLGIVLLGLFATKS
                     FNPAGVDGLFYGNPGFFVTEVGAVVLAVVWAFAFTVAMLWLINRVTPVRVGDAAEAAG
                     LDDSIHGEHAYVESL"
BASE COUNT         1285 a         2849 c         2896 g         1201 t
ORIGIN      
        1 tctggcgcgc gacgacgtcg tcggcccgga ccgcgccgat cagccgttgg gcgacctggg
       61 tgaggatctg gtcgccggcc gcgtggccgt aggagtcgtt gacgcgcttg aagtcgtcga
      121 ggtcggcgaa cagcagggcg accatcccgc ggttccgaac ctgccgggcc agcgcccgct
      181 cgaggtggtc gcggagcagg acccggttgg ccagcccggt cagcggatcg tggagggcat
      241 cgtggatgag cttcgactcc agcagcttgc ggtcggtcgt gtcgatgagc aggccctgcg
      301 agacctgccg cccactgtgg gtgtcgtcgg gcatcgcgta ggcctcgtcg cggacccaga
      361 cctcggagcc gtcgcgggcg agcatccggt actcggcccc caggggttga ccggtccgcc
      421 agtgatcggc gtacgcggcg atcgcggcct cgcggtcgtc ggggtggatc cggctgggcc
      481 acagctccgg atcgtccacg agctcgtcgg gtcgatagcc gaggatgccg ctgatctgcg
      541 ggctcatgta ggcgaggcgc cccccgtcgg agacgtcggg atcggccatg tcgatgtaga
      601 cggcggcggg gacgtgctcg acgagcaggc gcaggcgcga gccggcctcc tcgacttcgc
      661 gggtccttcg ctcgagggtc gccgctgagc ctcgcaggcg acgcaggagg acgacgccgg
      721 cgccgacgac gacgcccagg gcgacgacga cgccgacggc aatgacgggc ggcgcgatcg
      781 ggcccgatcc agcgccgatc aggccgatcc aggcgagcgc cgcaaggacg agtgcgccgc
      841 cgaacaaccc gaccgcgggg aggcggtcgg gcacgtcgaa gcgacgcatg ggccgatcca
      901 tccggtgacg ctacgcccgc gctgcgaccc cgcctaccac cgaatcgggc cagtacgcga
      961 caggagtgct aacgctcctc gtccgcgcgg tccgacgagt cgcgatcgcg gcgcagccag
     1021 ctcaagggtc gaccgctcgg gaccgcgtgc aggatcgact ccgaaccgtc gtcgttcgcg
     1081 ccctgcgcca ccgcgacaag cacgccggat tcggcgacgt cagctgccga gcccgcatcg
     1141 gtcgacccat cggccgatac gtcagccgac aactccagcg gtgcggccga gcccgaggcg
     1201 ttccacgcgg cgaacgacgc ggccagcctg gacccggagt cagggtccat cacaccgacc
     1261 ccggtcggct ctggggcgtc gttgtcctga tccgtcgatg gagcgtcgcc gacggaggtc
     1321 gccggcgtcg cgtccgcctc gccggcctgg tcgagcgtcg ggaagaccgg ccgctggctg
     1381 gcgtgctggg cgatcgcgac cggatcggtc tctcgatcca gcgcgccgaa gaaggcgtcg
     1441 acgtcgactc gataggtggc gatcgccgcc tcgaccccct cgccctcgcg gtcgaccttc
     1501 gcgccgtgct cggcaaggct cttctggagg tcctcgccga gcgccgccgc gcggcgctcg
     1561 tgctcgagct ggatccgctg tcgttcgtcc tcggcccagg catcgatcgc cttgaggtcg
     1621 tcggctgcaa gcccgcgcat cttctcggct tcagactcgc gacgcgtctg gatcgcggcg
     1681 aggtgctcct cgcggcggcg gtcggtctcc gtggcgatcc gggcccgctc tgcaccgacc
     1741 gtcgtctgca tcgcccggac gagctcgctg aggaaggtcg cggcatcgtc ggagctggtc
     1801 gacgtggccg ctggctccgc ggtcggatcg acgtccgcca cctccgtttc gggggcgacc
     1861 gcgatcgcat cctcgtgctg tgtcccgttc gcgctccatg aagcgccggt ctccgaggtg
     1921 gaggcgctgt cagacccgga ctctggctgc tcggacatgt cgaaacctcg tgtctccgcc
     1981 accccgcgac atcccggtcg tcgcgcaacc cgacgggcag cgatggtact gccgctcttg
     2041 acttcgggca acgcccagct cgatatcggg tcgcccgggg gcgggttcgt caagatagat
     2101 ccgatccgcc atcggtcgcc gtcaatgcat gtacgtccga gacccgacga acgtgtcgac
     2161 ggccgcaccg gtggtggagg gcatcgaatg cctcgcagaa tctggctggc agcgatcgtc
     2221 gcggccatcg cgtcgatggg cgtcgcgctc gcaccagcgg cggcggctga cccgggggtc
     2281 gccaacgggc ggatcgcctt cggcacccgc gcgaacggga gcttcaacat cgagacggtc
     2341 atgccggacg ggacggacct gaaggctgtg accagcggga ccggcttcca cctctgcccg
     2401 gactacacgc ccgacggccg gcagatcgtc ttctgcgcca acaccagcgg cgcattcgag
     2461 ctgtggacca tgaagcagaa cggcggcaag cccacgcagc tgacccactt cggggggttc
     2521 gcgacctttc cggacgtgtc accggacggc tcgaacatcg cgttcagcgg cttcctcggg
     2581 gacccggcga acgaccagat cttcgtcgtc gacgcggcca ccggcggggg agtcgtccag
     2641 ctgaccacgt gcccggatgc gcatccgtgg tgcttcagca gctacccggt ctggtcgccg
     2701 gacggcagca agatcgcgtt cagtcacgcc gacgggttcg acgccgacga cacgcccgag
     2761 aacgaacaga tctacgtcat gaacgcggac ggctcgaacc cgcacgcgat cacgaccggc
     2821 ccggccccga aggaccaggt ccccgactgg agcccggacg gctcgaagat cgtgttcacc
     2881 gtcggctact tcggcagcgg cgggatctgg accatcaatg ccgatggctc gggcgcccgc
     2941 cagctgaccg gttgcacggc atcggatccg acgccgtgcc cgaccggtga cgactggggc
     3001 acggcctggt cgcccgacgg caggaagatc gtgttcctcc gggatctgac gagcctcggc
     3061 atcgccgacc gccaggtcac gatcatcaac gccgatggca cgggcgcaca ctcgatcacg
     3121 acaccgggcc tccacgcggt cccggcatgg cagccccgcg gcgtcccgtc gggcggctga
     3181 gcgcacgcgc agcacaggcg acacgctcga ggccctgacg cgggctttga gcggtcgccg
     3241 acaggaggtc gggcatgggg acggagaacg cggaggtggt cacccgggca atgcacggtg
     3301 accacgacgc gttcgccgcc ctcatcggtc tggccacgaa ccggctctat gccctggcct
     3361 gcctcatcct ccgcgacccc gaccgggccg aggatgcggc gcaggaggcg atcgtgcggg
     3421 cctggcgtga gctgccccgt ctccgcgacc cggagaagtt cgatgcctgg ctgcgacggc
     3481 tgaccgtgaa cgcctgcttc gacgaggcgc gccggatcca ccgccgggca gaagtctctc
     3541 ttgttcgact cggcgatcgg gcgaccgccg atccggccgg agcgttcgtc gagcacgacc
     3601 gggtcgatcg cgcgttccgg cggctgccgt tcgaccagcg gacggtcctc gtcctgcagc
     3661 atcacttcga cctgtcgcac acggagatcg ccgagaccct cgggatcccg ctcgggacgg
     3721 tcaaggcccg gatccggtac ggctcggcgg cccttcgtgc gtccctcgac gccgacgacc
     3781 ggtcgtcgtt cgcggcctcg gaccggacgt cggcatgaac ggcaacggac cgagggaccc
     3841 catggccgat cggcgatcaa atcgcgacgt tgctccgatc ctccagacct ggatggcggc
     3901 ggtggcaccg ccgcgcgcgc cagagcggtt gctcgaggag gcgttcgccc ggacgatgac
     3961 cgctcggcag cttcgggtct atccgtggca cgtcgtccgg ctcggtcggc gaggaggctc
     4021 gtcgggcaac cggatcggtg ggatcgcctt catcggggtc gcggtcgccg tcgcgatcgc
     4081 tgtcgcggcc ggcctggttg tccggccggg gcagaacatc agcggccaac cgacgccgtc
     4141 cctcacgccg ccttcgccga gcccgagccc gagcccgccg gcgagcgcgg tcccattccc
     4201 gtccgcggtc gtcgtcgagc cgaccatcgc gatcccggtg accgggccga tcacgatcgc
     4261 gacggacggc agctcggtct ggttgttcac cgccacgggc gacctcgtcc ggatcgatcc
     4321 gcagaccaat gcggtcgccg cgtcggtcaa gctgaccccc gcgaccgacg cataccagtc
     4381 gctcgcgggg aatcagacgg ggctctgggt gaccgactgg gatacgaacc agctcctgcg
     4441 gttcgagccc cggacgctcc gatcggtgac gacgatcgac atgggatctc aaccgaaggg
     4501 cgtgctggtc accggaagtg cggtctggat cgccaacacc cgcggcggca gcgtgcaacg
     4561 gatcgatccg aagacgaaca agatcgcgaa gaccatcccg gtcgggccca cagggccctc
     4621 gggtccgaac tggctcgcgc agggctccgg cagcatctgg gtgggcatcc cgaacagcgg
     4681 gtccgtcgtc cggatcagcg agacgacgaa tgcgatcgag gcgacgatcg cgatcccggg
     4741 gccggcctct ccctgtggtg ggttggcggc cggaacgacg gcgatctgga tcacgagctg
     4801 tgacggcggc aacctcgtca cgcagatcga cccggccacg aacgcggtcg tcggcacgat
     4861 cgatcttggc ggccgcggct atacgttcgc gatgatcgcc gaccgaccgt ggatctcccc
     4921 gacggacggt cagatcgtcc gcgtcgaccc cgtccgccat gtcgtggacc gagtcatcgc
     4981 accgggaacc ggcttctccg gcggcggcga tgtcgtggcc gcagcgggtt cgttgtgggt
     5041 catcgacggg gcggcgaatc gcgtcctccg cttgccgatc gccgccttcg gcggctgacc
     5101 accggagcgt gctcagcggc ggctcgccac ccgcgatgcc gcattcgtgg cgaatgcctg
     5161 caggacgagc ccgtgtttga cgcccaccga atcgacgacg acgacggcct cgccatccgc
     5221 cgtgacgtgg cggcccacct tgtctctgac ctcgaagcgg ccgccccgga gtcggcggat
     5281 gcggagatcc gcatgcgatc cccgatcggc cacgattccg gtcaccttcc gcgtgtccgg
     5341 ggcccggacg aacgcgatcc ccaggtgcga cccgtgtccg acattggcgg tgtgaacgac
     5401 gtcgaccacc gcgacgacgt gacccagatt tcgtccgcgc agcagtgcga cgagagccac
     5461 cagcacgcca ccggccagca gcagcccgag gccgccgagg accggcagga gcagcgagtt
     5521 gcactcgagg gtcgagccgc acggcttgac ggcatccgac agcggcgatg cttcgctctg
     5581 gccgagggcg ttcttggcga aggcccggca ggtgtagtcg acgccgttcg tgaggttgcc
     5641 gatctggccc gatgtatcgt ccggcgcggc gtcgaccgtc gccggccatg tgccaccctg
     5701 gtcactcgag cattcgtagt ggtagctggt cacgtcctgt ccgccggcgg gcgtgacggc
     5761 gatccgcagg gcgtggttca gcgcctcgac cgagggcttg gccggtgctg ccggccggcc
     5821 gaagggcgtg accgacccag ctgacgccgt ccaggggccc gcggctgacg ggccgacggc
     5881 cgccacttcg cactggtacg cggtcccgtt ggtcagtcct tcgattgtcg cggtcggctc
     5941 gagcgagacg ccttcggtcg attcgatcca gtccccgtcg ccggcgcggc agcggacggc
     6001 gtaatcgacg atgggcgccg ccgtggcggc agcgggagtc caggtcagct cgatccggcc
     6061 gtcgccggcg gtgagtcgga tgcccttgac cgcggcgggc gtcggaacgg gggtcggggt
     6121 gggcgtcgga gcggcggtga acgtgcaggt cgcgtcctgg agggcgacgg cgacggcgtt
     6181 gcccgcctga tcggccgtct ggaagaccac ctgcggccaa tcgaacgtgg cgccgctgat
     6241 gcaggccggc atgtcgcgga tgatccgcag cccttcgagg ccgctcgtga gggcactgcg
     6301 cttcttcggg tcgaggccca tgccgaccgg cagcagcgcg aacggcgagc ggctcccgaa
     6361 cagccgctcg agggcgacgt ggacctggct gacgggcacg cccttgacgt cgtgcttgcc
     6421 gtcggtgaac aggatcatcg ccggccggac ggcgtccgcg ggaaggtgcc gggcggccgc
     6481 ctccatcgcc gcgacgtagt tcgtatcgac gccgatcttc cccgtgaggg cggacgtgag
     6541 ccccttgcgg taggcgacgg caacggatcg aaggcagtcg gcgaatcggg cgacggtcgg
     6601 cgcgcggtcg agcagcttca ggtcggtgca gcccgggtag ccggccgccc gcgtggcgaa
     6661 ctggacgatc gtgaccgtgg tatcgcccgc gacgaggtcc gtggagatct cgtcgacgcg
     6721 atccgcgatc cgctcgagcg ccgcgccgaa gcggttccgg ttggtcttgt cgtcgaggat
     6781 cgaggccgag aagtcgagga cgaggacgac gtcggaccga tccggaccct gggcgcccac
     6841 atgcggcacg aggccgccgc cgacgaggac gattgcggcg gccaaagcgg cgacgacttg
     6901 tgcccccgga ccgaggcgtc tcatggcaag tggctgtcct tcttctgggc gcattcattg
     6961 tggcccgcgg gacgggacgc cgccaagcag ggagttggcc gcgccttaca gcgactcgac
     7021 gtacgcgtgc tcgccgtgaa tcgaatcgtc gaggcctgcc gcctcggccg catcgccgac
     7081 ccggaccggc gtcacccgat tgatcagcca cagcatcgcg acggtgaacg cgaaggccca
     7141 gaccaccgcg aggacgaccg ctccgacctc ggtcacgaag aagcccgggt tgccgtagaa
     7201 gagcccatcg acgccggccg ggttgaacga cttggtcgcg aacaacccga gcaggacgat
     7261 gccgagcgat ccccccacgc catggacgcc ccagacgtcg agcgcgtcgt cgaggcggag
     7321 ccggttcttg agggccaccg ccagatagca gaccactccc gcgaggcacc cgatgatcac
     7381 cgcagtcacc ggcgagacgt agccggcggc cggggtgatc gtggccaggc ccgccacggc
     7441 gccggtcagg aggccaagga acttcggttg tccggtgtgt cgccattcga tcgccagcca
     7501 gaccatcgcc gcgaacgacg cggcaatgtc cgtgttcagg aacgcgaccg cggtcacgga
     7561 gtcgacgcgg aactcgctgc cggcattgaa tccgtaccag ccgaaccaca acaggccggt
     7621 tcccagggcc acgagcggga tgctgtgggc cccgccgtcg ggactgtggc gacggccgac
     7681 gtagagcacc gacgcaaggg ccgccgtccc ggcaatcgca tggacgacga tgccaccggc
     7741 gaagtcaagc acgccccgtt cggccaggaa tccgccgccc cagatcatgt ggacgaacgg
     7801 gaaatagacg aacagcagcc agccggtcag gaacagcatg tacgccttga acgtgacccg
     7861 gttggcgaat gccccggtga tcagggcagg ggtgatgatc gcgaacatca tctggtaggc
     7921 gatgaagacg atggccggga tcgtgtcgtt gatcgtcgat ggcgtgtcga gcgtcacgcc
     7981 attgaggaac gccatgttga ggtcgccgat gattccgccc tggccgccgc tgaagcacag
     8041 cgagtagccg acgacgaccc acagcacggt cgtccagccc atcgagacgt agctctggat
     8101 catgattccg aggacattcc ggcggctgac caacccgccg tagaagaacg ccagacccgg
     8161 tgtcatcaac atcacgaggc tcgtgcacaa gagcatgaag cccgtgttcc cggtatcgag
     8221 gttcggcacg a
//
LOCUS       DATAIW010000096         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_270095, whole genome shotgun sequence.
ACCESSION   DATAIW010000096 DATAIW010000000
VERSION     DATAIW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270095"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VHM48_03250"
     CDS             <1..446
                     /locus_tag="VHM48_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2754451.1"
                     /translation="PEIDVFGAGLGTSGTLMGVGRFLRREKAGVRIVAAEPLPGENVQ
                     GLRSLEEGFVPEILDPAMLDAKYLVSNLDAIAALRDLVEREGIFGGPSCGAVLVAAAR
                     EAQTMTSGTIVALLPDGGWKYLSAGTFSRDFADMADDLEGGVSWW"
     gene            516..758
                     /locus_tag="VHM48_03255"
     CDS             516..758
                     /locus_tag="VHM48_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2754452.1"
                     /translation="MNDRTIMASRFKAECLAILDQVDQLKISVIVTKHGRPVARLVPM
                     DSGSTADLVGSVTLVAEDDEAYYSTGASWEAEGRSP"
     gene            755..1159
                     /locus_tag="VHM48_03260"
     CDS             755..1159
                     /locus_tag="VHM48_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2754453.1"
                     /translation="MTPLLDTHVLLWWLSGGSRLSSAAAAAIESAERVLVSPISCWEI
                     GMLARAGRIRLDRPIASWIARVFDDRRAAVAQLSPEAAGWAGALDDDRFPGDPADRLI
                     YATALEHRIPLISKDERLREFASVAGDVRVVW"
     gene            1140..1595
                     /locus_tag="VHM48_03265"
     CDS             1140..1595
                     /locus_tag="VHM48_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2754454.1"
                     /translation="MSVSSGDRPGPASVHLPVELVEAIVRHARAEDPNEACGLIVGDR
                     PAAKGGAALRFEPTRNRAASPYRYEIHPDDLLRLTIATDDADQVFWAIVHSHVRSPAY
                     PSPTDVGLAFYPDALYLLVSLAEDEPALAAFRIVDGQIFPVELLVGAGS"
     gene            1592..2257
                     /locus_tag="VHM48_03270"
     CDS             1592..2257
                     /locus_tag="VHM48_03270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2754455.1"
                     /translation="MTGARRVAPGTLDPRQVVADGYDRMADRYLSWSALRPSVARLRA
                     LGLADELIPAGADVLELGCGAGLPMTAALAVGRRLTGIDISAEQIRRARRNVPTATFI
                     QADLASLDRPAASVDAVVAFYSLTHVPRDEHAALFVRIRRWLRPGGLFIASLGVEDDP
                     GGIESDWLGVDMYFSHFSARVNRRLLETAGLVIERADVIVEPEDRHDARFLWVVARAP
                     GPS"
BASE COUNT          331 a          895 c          871 g          409 t
ORIGIN      
        1 gcccagagat cgacgtgttc ggcgccgggc tcggaacgag cgggaccctg atgggcgtcg
       61 gtcgattcct gcgccgggaa aaggccgggg tccgcatcgt cgccgccgag ccactcccgg
      121 gcgagaacgt ccagggcctg cgatcgctcg aggagggctt cgtgcccgag atcctcgacc
      181 cggccatgct cgacgcgaag tacctcgtca gcaacctcga cgcgatcgcc gcattgcgcg
      241 acctcgtcga acgggagggg atcttcggcg gtccgtcatg cggagcggtg ctcgtcgcgg
      301 cggcgcgcga ggcccagacg atgacgtccg gcacgatcgt cgcgctcctg cccgacggtg
      361 gctggaagta cctgagcgca ggcacgttca gccgcgactt cgccgacatg gccgacgacc
      421 tcgagggcgg cgtcagctgg tggtgagcgg cgcctgatca cggggcgatg ggaggcgatt
      481 gacgccaata tgacctgtga ctatagtcaa tggtcatgaa cgaccgaacg atcatggcga
      541 gccgcttcaa ggccgaatgc ctcgcgatcc tcgaccaggt cgaccagctg aagatctcgg
      601 tcatcgtgac gaagcacgga cgccccgtcg ccaggctcgt gccgatggat tcaggctcga
      661 cggccgacct ggtcggaagc gtcaccctcg tggcggagga cgacgaggcg tactactcga
      721 cgggggcgtc gtgggaggcc gagggtcgtt cgccgtgacc ccgctcctcg acacccatgt
      781 cctcctgtgg tggctgtcgg gaggatcccg gctctcctcg gcggcggcgg cggcgatcga
      841 atcggcagaa cgcgtcctcg tgagcccgat ttcgtgctgg gagatcggga tgctcgcccg
      901 ggcggggcgc atccggttgg accgtcccat cgccagctgg atcgcccgtg tcttcgacga
      961 ccgccgcgcg gccgtcgccc agctctcgcc ggaggccgcc ggctgggccg gcgccctcga
     1021 cgacgaccga ttcccgggcg atcccgccga ccggctgatc tacgccacgg cccttgagca
     1081 ccgcattccg ttgatcagca aggacgagcg gcttcgggag ttcgcgagcg tcgccggcga
     1141 tgtccgtgtc gtctggtgat cgccccgggc cggcgtcggt ccatctgccg gtcgagctgg
     1201 tcgaggccat tgtccgccac gcccgggccg aggatccgaa tgaagcctgc ggcctgatcg
     1261 tcggtgatcg accggccgcc aagggtgggg ctgccctgcg cttcgagccc accaggaatc
     1321 gggccgcctc gccataccgc tacgagattc atccggacga cctcctgcgt ctgacgatcg
     1381 cgaccgacga cgccgaccag gtgttctggg caatcgtcca cagccacgtc cgatcgcccg
     1441 cctacccgag cccgaccgat gtcggactcg ccttctaccc cgacgccctg tacctcctcg
     1501 tgtcactcgc cgaggatgag ccggcgctgg ccgcgttccg gatcgtggac gggcagatct
     1561 tcccggtcga gctgctcgtc ggggccggct cgtgaccggg gcacgccggg tggcgccggg
     1621 cacgctcgat ccgcggcagg tcgtcgccga cgggtacgac cggatggccg accggtacct
     1681 gtcctggagc gccctccgtc cgtcggtggc gcggctgcgc gcgctcgggc tcgccgacga
     1741 gctgatcccg gccggcgccg acgtcctcga gctcgggtgc ggtgccggtc tgccgatgac
     1801 ggcggccctc gcggtcggtc ggcggctgac ggggatcgac atctccgccg agcagatccg
     1861 gcgggctcgc cgcaacgtcc cgaccgcgac gttcatccag gccgacctcg cctcgctgga
     1921 ccggccggcc gcgagcgtcg acgcggtcgt cgcgttctac tcgctgacgc acgttccgcg
     1981 cgacgagcac gccgccctgt tcgtgcggat ccggcgatgg ctccgtcccg gcggactgtt
     2041 catcgcctcg ctcggcgtcg aggatgaccc cggtggcatc gaatcggact ggctcggcgt
     2101 cgacatgtac ttcagccact tctcggcgcg ggtcaaccgc cgtctcctcg agacggccgg
     2161 gctcgtcatc gagcgggccg acgtcatcgt cgaacccgag gaccgccacg acgcgcggtt
     2221 cctgtgggtg gtcgcccgcg cgccggggcc gtcgtgagcg ccgtggtcct gatcatcgcc
     2281 ggggtcgacg tcctcgagac ctcgttcctg ctcctcgtcg tcggcctgcg cacgcggcgc
     2341 tgagcggctc ggtcctgcaa ccggtcattg acgagtggcc gggatcgtcc tagcgtccga
     2401 gcctgccgcc ggccgtgttc gcccggacac agccgccgtc gtgcccgttc gccggaggct
     2461 ccgcgatgct cggttcaagc ccgtctcatt cgcttccggt cctcgt
//
LOCUS       DATAIW010000097         9151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_4205, whole genome shotgun sequence.
ACCESSION   DATAIW010000097 DATAIW010000000
VERSION     DATAIW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VHM48_03275"
     CDS             complement(<1..1026)
                     /locus_tag="VHM48_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754456.1"
                     /translation="MATLKRVAHDGFASFVGFGRGIWLFPLDMTFRTVGLRYGWLKAL
                     FNATPAPLLRGIGRLRAERAAWRATRSVPAYGRFLAESGVNVASLFPLGVLGHLPETD
                     KASYVDRYGLLERCVGGAVPYPGTTIDESSGSTGTPYNWIRSRREREVAHRNIGFFAR
                     YAFGTAPLVTINAFSMGAWAAGFNMSLGMLRHGIVKSVGPDVDKIFSTLALLGPTYRF
                     LISGYPPFLKHLLDEGERRGFPWADYELHALVGGEGMTEGLRDVLLGRFVSVYSGYGA
                     TDIEIGMAGESPVSIAVRRLARARPDIARALFGTDSRLPMVFQYNPLIHFMEVNNLGE
                     VICTVSRL"
     gene            1373..2509
                     /gene="ruvB"
                     /locus_tag="VHM48_03280"
     CDS             1373..2509
                     /gene="ruvB"
                     /locus_tag="VHM48_03280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX2754457.1"
                     /translation="MTDDLSRILAPDLLDDTDVVVETTLRPRTLDEYIGQREVKANLS
                     VLLAAARGREEAADHVLLYGPPGLGKTTLATIIARELGVNVRYTSGPAVERAGDLAAI
                     LTALDERDVLFIDEIHRLNRAVEEILYPAMEDYALDVMIGKGPSARSLRLNLKPFTVV
                     GATTRAGRISSPLRDRFGATYRLDFYEEDDLAAIVDRSARILDVPIDDAAARAIARRG
                     RGTPRIVNRLLKRTRDHAQVHGNGRVDEATATAAMRQMEIDDEGLDSTDRKLLAAIIQ
                     KFASGPVGVQALAAVLAEEVETIEDVYEPFLLRLGFLDRTPQGRIATEAARNHLARLG
                     YEIPPPRRPEPELVGLWDLPVDPAEAVDPGASEPGASDPGAPEA"
     gene            2506..3663
                     /gene="tgt"
                     /locus_tag="VHM48_03285"
     CDS             2506..3663
                     /gene="tgt"
                     /locus_tag="VHM48_03285"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX2754458.1"
                     /translation="MTAARFTVEVPPPADGATRARVGRLELSHGVVETPQFMPVGTNA
                     TVKALHPDDLREVGASIILSNTYHLYLRPGHERIARLGGLHRFMAWDGPILTDSGGFQ
                     VVSLGDLRVVDDDGVTFRSHLDGSTHRFTPEVAIAVQEALGSDIAVAFDQPVYPSSPR
                     EVVADATARTHRWAERSLAAHTRPDQALFGIVQGGLEPDLRAESTRYIAALPFDGICL
                     GGLAGDETPEQRNATLDVAVPLLDDDPRPRYLMGLGSPSDLLDAVQRGVDLFDSVLPA
                     RVARNGQLWVPGGRLNLQNARFLDDPAPVQEDCPCLLCRTFSRAYLAHLFRAKELLAY
                     RLATCHNLTFTLDFMLRIRTAIRSGIFPAALPDLALRAGRSSASEVAVQSA"
     gene            3706..3873
                     /locus_tag="VHM48_03290"
     CDS             3706..3873
                     /locus_tag="VHM48_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754459.1"
                     /translation="MGSRDRPSKEQKKKPKDRSGQPKLTSLSEPPPKQAELIRKPRKE
                     KPVREEETDEG"
     gene            3943..5028
                     /gene="recA"
                     /locus_tag="VHM48_03295"
     CDS             3943..5028
                     /gene="recA"
                     /locus_tag="VHM48_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2754460.1"
                     /translation="MALIGERAADERNGSDRASDRAQAERTKAVSAAIATIEKQFGTG
                     SIMKLGSGSIQAIDVIPTGSIALDLALGVGGIPRGRITEIFGPESSGKTTICQHVIAE
                     AQRRGGVAAFIDVEHALDPAYARACGVDVDELLVSQPDTGEQALEITETLIRSGGIDC
                     VVVDSVAALVPRAEIEGEMGDSFVGIQARLMSQALRKLTGAVSRSHTSLIFTNQLREK
                     IGVMFGNPETTPGGRALKFYASVRLDIRRIETIKTGTESVGNRVRVKVVKNKVAPPFR
                     VAEFDVMYGEGVSKEGGLLDVGVAMSVVTKTGAWFTFEETRLGQGREASKDFLRANPD
                     IAAVIDAKIRGKINEVDLPVEGIAEAE"
     gene            5110..5631
                     /locus_tag="VHM48_03300"
     CDS             5110..5631
                     /locus_tag="VHM48_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX2754461.1"
                     /translation="MVLEAAARFLEARSRSVAEVRRRLGRAGYRAELIDGAITRLTEL
                     GMLDDEAFGRAWLESRDRARPRGEIALRRELALKGVGRAIVDELLDERRDAGGTEADG
                     VGVDLAAARRLLTRNERALARVADPRQRRQRAYALLARNGFDPETCRDASAALVATVD
                     DPTPGESDLAEDD"
     gene            complement(5641..6888)
                     /locus_tag="VHM48_03305"
     CDS             complement(5641..6888)
                     /locus_tag="VHM48_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2754462.1"
                     /translation="MNRARSVETVVIGAGQAGLALSWFLRQAGREHVVLERRTTLGGG
                     WQDRWDAFCLVSPNWTTGVPGFDYDGDDPDGYMPRDEIVARFRRYATAIAAPVELGIA
                     VTGLTRTDPRRPGFRLETSGGPIEAREVIVAAGGFHAPKIPAAASGFGPRILQLHSHQ
                     YRNADALPHGAVLVVGTGQSGVQLAEELQAAGRQVFLSVGHCGRAPRRYRDRDIFWWL
                     RQFATKGPSVGAPLPTVDRLPDPRMRFACNPHLSGHAGGHTTNLRRFAADGMRLVGRF
                     ESGEGERARFAADLTANLRFADGFFDERLRPLCETLIERLGLELPPHVDDPFVFEPPE
                     VTELDLAAEGISTVLFTSGYRLDFSWIDLPIFEESGLPVHVRGVTEVPGLTFIGLPWQ
                     YSMGSANLVSFAQDAAYLAERWS"
     gene            7142..8671
                     /gene="rny"
                     /locus_tag="VHM48_03310"
     CDS             7142..8671
                     /gene="rny"
                     /locus_tag="VHM48_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX2754463.1"
                     /translation="METLVVVAVALIAAVGGVVLGMIARGMFASNSMKAAQSEARRIE
                     AEARTRQKELILEAKDEKLKMQREAEDEARAKRSELSTLERRLLQRDEQLDQRTDMLE
                     QRDRKLLERERDLDKTREELAKAHQEQVVALERVSGVSAEEAKGILLEAVRDEAEHDA
                     VKLARSIERRAREEADEKARDVVVTAIQRVAADHTAEHTVTVVHLPNDEMKGRIIGRE
                     GRNIRALEQATGIDLIIDDTPESVVLSGFDPVRREVARIALTKLIGDGRIHPGRIEEV
                     VAKARAEVELVIRQAGETAAYECGVPGLPPELIKLLGRLKYRTSYGQSVLNHVIETSR
                     LAAVIAAEVGADIQIAKTGGLLHDIGKAVDHEIEGPHAAIGATIAQRNNLSPKVINAI
                     GAHHQEIEYTCVEAPIVQIADAISASRPGARGETMDAYVKRLEDLQAIADSFAGVERS
                     FAVQAGREVRILVRPEEIDDLTATRLARDIVRKIEEQLTYPGQIKVTVIRETRAVEYA
                     K"
     gene            8725..8988
                     /locus_tag="VHM48_03315"
     CDS             8725..8988
                     /locus_tag="VHM48_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754464.1"
                     /translation="MTGRGLQGRHLARLLLLAAFALDLIPAFLFVSAPNFIGPMSAEP
                     PSRLVWLIPAVGIALNLAGLAWMVRLYLSDSEARPSAFRFTHR"
BASE COUNT         1498 a         3242 c         3152 g         1259 t
ORIGIN      
        1 gagccgcgag acggtgcaga tcacctcgcc gaggttgttg acttccatga agtggatcag
       61 ggggttgtac tggaacacca tcggcaggcg ggaatcagtc ccgaacaagg cccgcgcgat
      121 gtccggccgg gcacgcgcca ggcggcgcac ggcgatgctc accggcgact cgccggccat
      181 gccgatctcg atgtcggtcg cgccgtagcc ggagtagacc gagacgaacc gcccgaggag
      241 gacgtcgcgc agcccctccg tcatcccctc gccaccgacc agggcgtgga gctcgtagtc
      301 ggcccacggg aagccacgcc gctcgccctc atcgagcaag tgcttgagga acggcgggta
      361 gcccgagatc aggaagcgat acgtcgggcc gagaagggcg agggtcgaga agatcttgtc
      421 gacatcgggc ccgaccgact tgacgatccc gtggcggagc atgccgaggc tcatgttgaa
      481 gccggccgcc caggccccca tcgagaaggc gttgatcgtg accagcggcg cggtcccgaa
      541 cgcgtagcgg gcgaagaagc cgatgttgcg gtgggcgact tcgcgctcgc gccggctgcg
      601 gatccagttg tagggggtgc cggtcgagcc gctcgactcg tcgatcgtcg tgccgggata
      661 ggggaccgcc ccgccgacgc agcgctcgag caggccgtac cggtcgacgt agctcgcctt
      721 gtccgtctcc ggcaggtggc cgaggacgcc gagcggaaag aggctcgcga cgttgacgcc
      781 gctctcagcc aggaagcggc cataggccgg gacagagcgg gtcgcccgcc aggccgcccg
      841 ctcggcacgg agccggccga tcccgcggag gagcggcgcc ggcgtcgcgt tgaacagcgc
      901 cttgagccag ccgtagcgaa gcccgaccgt ccggaacgtc atgtcgagcg ggaacagcca
      961 gatgccgcgc ccgaacccga cgaacgaggc gaacccgtcg tgggctaccc gcttcagcgt
     1021 tgccattgcg tcgatgctcc tggccagtgc ggtgcggtcg agtgccgtta tatcccgacc
     1081 acgtatgcgg gaacagtctg ttacgtcctg ggcgggctgc tgcgtgaaat gcgcatgtcg
     1141 ggcgggcgag tcgctgaggg tgcggcgagc cgggcctgac tcaccgcgtg agtcaatggg
     1201 ccctcgacca cgctcggtcg cgtccgattc gtgaccgagc cccgcacgtc gaacctggtc
     1261 ttgactcacc ggctgagtca gaccggcgga attggggagt caaattgcgc gaacgcatcg
     1321 acatgataga acatccgtgc gaaatggctc ggagcatggt acattccgcg cgatgacgga
     1381 cgacctctcg cggatcctcg cgccggacct cctcgacgac acggacgtcg tcgtcgagac
     1441 caccctccgg ccgcggacgc tcgacgaata catcggccag cgtgaggtca aggccaacct
     1501 ctcggtcctg ctcgcggcgg cgcggggcag ggaagaggcg gccgaccacg tcctccttta
     1561 cggcccgccc ggcctcggca agacgacgct tgcgacgatc atcgcccgcg agctcggggt
     1621 caacgtccgc tacacgagcg ggccggcggt cgagcgggcc ggtgacctgg ccgcgatcct
     1681 gaccgcgctc gacgagcgcg acgtcctctt catcgacgag atccatcgcc tgaaccgggc
     1741 ggtcgaggaa atcctgtacc cggcgatgga ggactacgcg ctcgacgtga tgatcggcaa
     1801 ggggccgagc gcccgcagcc tgcgcctgaa cctgaagccg ttcacggtcg tcggggcgac
     1861 gacgcgcgcc ggccggatct cgtctccgct gcgcgaccgc ttcggcgcga cctaccggct
     1921 cgacttctac gaggaggacg acctggcggc gatcgtcgac cgttcggcgc ggatcctcga
     1981 tgtccccatc gacgacgccg cggcccgggc catcgctcga cgcggccggg ggacgccgcg
     2041 gatcgtgaac cgcctcctga agcggacccg cgaccacgcc caggtccacg gcaacggccg
     2101 cgtcgacgag gcgaccgcca ccgccgcgat gcgccagatg gagatcgacg acgagggcct
     2161 cgactcgacc gaccggaagc tgctggcggc gatcatccag aagttcgcgt ccgggccggt
     2221 cggcgtccag gcgctggcgg ccgtcctcgc cgaggaagtg gaaacgatcg aggacgtcta
     2281 cgagccgttc ctcctccggc tcgggttcct cgaccggacc ccgcagggcc ggatcgcgac
     2341 cgaggcggct cgcaaccacc tcgcccggct cggctacgag atcccgccgc cacgccggcc
     2401 ggaacccgag ctggtcgggc tgtgggacct gcccgtcgat cctgccgagg ccgtcgatcc
     2461 aggcgcgtcc gagccaggcg cgtccgatcc aggggcgccc gaggcgtgac cgccgcccgc
     2521 ttcacggtcg aggtcccgcc ccccgccgac ggcgccacgc gggcccgcgt cggccgtctg
     2581 gagttgtccc atggcgtcgt cgagacgccg cagttcatgc ccgtcggcac gaacgcaacg
     2641 gtcaaggcgc tccaccccga cgatctgcgg gaggtcggcg cctcgatcat cctctcgaac
     2701 acgtaccacc tctacctccg gccgggccac gaacggatcg cccggctcgg cggcctgcat
     2761 cggttcatgg cctgggacgg cccgatcctg accgactccg gcggcttcca ggtcgtgtcg
     2821 ctgggcgacc tgcgggtggt cgacgacgat ggggtcacct tccgcagcca cctcgacggc
     2881 tcgacccatc gcttcacgcc ggaagtcgcg atcgcggtcc aggaggcgct cggctccgac
     2941 atcgccgtcg cattcgacca gcccgtctac ccgagctcgc cgcgagaggt cgtcgccgat
     3001 gcgacagccc gaacccatcg ctgggcggag cggtccctcg ccgctcacac gcgcccggac
     3061 caggcgctct tcgggatcgt ccagggcggc ctcgagccgg atctccgggc ggagagcact
     3121 cggtacatcg ccgcgctccc gttcgacggc atctgtctgg gcgggctggc cggcgacgag
     3181 acgcccgagc agcgcaacgc cacgctcgac gtggccgtcc cgctgctgga cgacgacccg
     3241 cgaccgcgct acctgatggg tctcggctcg ccctccgatc tcctcgacgc ggtccagcgc
     3301 ggcgtcgacc tgttcgattc ggtcctcccg gcgcgggtcg caaggaacgg ccagctgtgg
     3361 gttcccggcg gtcggctcaa cctccagaac gcccgcttcc tcgatgaccc ggcgccggtc
     3421 caggaggact gtccgtgtct cctctgccgg acgttttcgc gggcctatct ggcccatctc
     3481 ttccgggcga aggagctcct cgcctaccgt ctcgcgactt gtcacaacct gaccttcacc
     3541 ctagacttca tgctcaggat ccgcaccgcg atcaggtccg gaatcttccc cgcagcgttg
     3601 cccgatctgg ccttgcgcgc cggccgatcg agtgccagtg aagtcgcggt gcagtccgcg
     3661 tgaatggtca accggtagcg tccagctcaa cccggaggag catcgatggg ctctcgagat
     3721 cgtccgagca aggaacagaa gaagaagccc aaggacaggt cgggccagcc caagctgacg
     3781 tccctttcgg agccgccgcc gaagcaggcg gagctgatcc ggaagccgcg caaggaaaag
     3841 ccggtccgcg aggaagaaac cgacgagggt tgaacacccg aggtctcgcc cgggagcgcg
     3901 gcctgtcggt ccagaagcca tcgacaggag cgtgacgaac ccatggcgtt gatcggagag
     3961 cgggccgccg acgagcggaa cggcagcgat cgagcgagcg accgagccca ggccgagcgg
     4021 accaaggcgg tctcggccgc catcgcgacg atcgagaagc agttcgggac gggctcgatc
     4081 atgaagctcg gctcgggctc gatccaggcg atcgacgtca ttccgacggg ctcgatcgcg
     4141 ctcgacctgg ccctcggtgt cggtggcatc ccgcgcggcc ggatcaccga gatcttcggg
     4201 ccggaatcct cgggcaagac gacgatctgc cagcacgtca tcgccgaggc ccagcggcgt
     4261 ggcggcgtgg ctgcgttcat cgatgtcgag cacgccctcg atccggccta tgcccgggcc
     4321 tgcggcgtgg acgtcgacga actgctcgtc agccagcccg acaccggcga gcaggcgctc
     4381 gagatcaccg agaccctgat ccggtccggc ggcatcgact gcgtcgtcgt cgactccgtc
     4441 gcagccctcg tgccgcgagc ggagatcgag ggcgagatgg gcgacagctt cgtcggcatc
     4501 caggcccgct tgatgagcca ggcgctccgg aagctgaccg gcgccgtgtc ccgctcgcat
     4561 acgtcgctga tcttcacgaa ccagctccgc gagaagatcg gcgtgatgtt cggcaacccg
     4621 gaaacgactc cgggtggccg cgccctgaag ttctacgcca gcgtccgcct cgacatccgc
     4681 cggatcgaga ccatcaagac cggcaccgag tcggtcggca accgggtccg ggtcaaggtc
     4741 gtgaagaaca aggtcgcgcc gccgttccgg gtcgccgagt tcgacgtgat gtacggcgaa
     4801 ggcgtgtcca aggaaggcgg cctcctcgac gtcggcgtcg cgatgagcgt cgtcaccaag
     4861 accggcgcct ggttcacctt cgaggagacc cggctcggcc agggtcgcga ggcgtcgaag
     4921 gacttcctcc gcgcaaatcc ggacatcgcc gcggtcatcg acgcaaagat caggggcaag
     4981 atcaacgagg tcgacctgcc ggtcgagggt atcgccgagg ccgagtaggt cttcccggtg
     5041 cccggccgtc gaccgaggga atccattgcc gacggccgcg agcgcagagc cgcgatcgac
     5101 gatccggccg tggttctcga ggcggccgcc cgcttcctgg aggcccgctc gcggtccgtg
     5161 gcggaagtcc gccgccggct cggccgggcc ggctatcgcg cggagctgat cgacggcgcg
     5221 atcacccgcc tgacggagct cggcatgctc gacgacgagg cgttcgggcg ggcatggctg
     5281 gagtcgaggg accgggctcg gccgcgcggc gagatcgccc tccgacgcga gctcgcgctc
     5341 aaaggagtcg gtcgagcgat cgtcgacgag ctgctcgacg agcgacgtga tgccggtggg
     5401 accgaggcgg acggagtggg cgtcgacctc gcggctgcgc gccggctgct cacccgcaac
     5461 gagcgagccc tcgcccgggt cgcggatccg cgccagcgcc gccagcgcgc ctatgcgctg
     5521 cttgcccgca acggcttcga tcccgagacc tgtcgtgacg ccagcgccgc tctcgtcgcg
     5581 acggtcgacg accccacccc cggggagagc gacctggccg aggacgactg accgccggcc
     5641 ctacgaccag cgttcggcga ggtacgccgc gtcctgggcg aacgagacga ggtttgccga
     5701 gcccatcgag tactgccacg gcaggccgat gaaggtcagc ccggggacct cggtcacgcc
     5761 gcggacatgg acgggcagcc cgctctcctc gaagatcggc aggtcgatcc acgagaagtc
     5821 gaggcgatag ccgctcgtga agaggaccgt cgagatgccc tcggcggcaa ggtcgagctc
     5881 ggttacctcg ggcggctcga acacgaatgg atcgtccacg tgcggtggca gctcgagccc
     5941 cagccgctcg atgagggtct cgcagagggg ccggagccgc tcgtcgaaga acccgtcggc
     6001 gaagcgcagg ttggccgtga gatcggccgc gaaccgcgcc cgctcgccct caccggactc
     6061 gaaccggccg acgaggcgca tcccgtcggc tgcaaagcgg cgcaggtttg tcgtgtgacc
     6121 cccagcgtgg ccggacaagt gggggttgca ggcgaaacgc atcctcggat ccggcagccg
     6181 atcgaccgtc ggcagtggcg ctccgacgga cgggcccttg gtcgcgaact ggcggagcca
     6241 ccagaagatg tcgcgatcgc ggtagcgtcg gggagcccgg ccgcagtggc cgaccgacag
     6301 gaacacctgt cgtcccgcgg cctgcagctc ctccgcaagc tggacgccgg actggccggt
     6361 tccgacgacg aggaccgccc cgtgcggcag cgcatcggcg ttccggtact gatgcgagtg
     6421 gagctgcagg atccggggac caaagccgct cgcggcggcc gggatcttcg gcgcatggaa
     6481 tccaccggca gcgacgatca cctcgcgggc ctcgatcggc ccaccgctcg tctccaaccg
     6541 gaaacccggg cgtcgcgggt ccgtccgggt cagcccggtg accgctatcc cgagctcgac
     6601 cggtgccgcg atcgccgtgg cgtagcgccg gaagcgggcc acgatctcgt cgcgcggcat
     6661 gtacccgtcg gggtcgtcgc cgtcgtagtc gaacccggga actccggtcg tccagttcgg
     6721 ggagacgagg cagaacgcgt cccagcgatc ctgccagccg ccgcccaggg tcgtgcgccg
     6781 ctcgaggacg acgtgctccc gcccggcctg acgcaggaac cagctcagcg cgaggccggc
     6841 ctggcccgcc ccgatcacga cagtctcgac cgagcgcgcg cggttcatcc ccatggatcc
     6901 ggatctccct cgcgctgaca cgccgcaacc cggcggcaac ccgtcggccg ctcgacgggc
     6961 cgcaccgtag taggctacat cccagatttc aacaagcgcc gtcggagacg gcgcggccca
     7021 ggtaaccggt gttcgtcttc gcctacgacc ggcccatcgg tcgatgtcgc cgttcccgtc
     7081 gagacgacgc cgcaccaccg atcgtcaacc tccggtgccc gacgccggac gggaggaaca
     7141 gatggagaca ctggtcgtcg tcgcggtggc gctcatcgcc gccgtcggcg gagtcgtgct
     7201 cggcatgatc gcccgcggca tgttcgcctc caactcgatg aaggcggccc agtccgaggc
     7261 gcgccggatc gaggccgagg cgcggacccg ccagaaggag ctgatcctcg aggccaagga
     7321 cgagaagctg aagatgcagc gcgaggccga ggacgaggcg cgcgccaagc gcagcgagct
     7381 gtcgaccctc gagcgacggc tgctccagcg cgacgagcag ctcgaccagc ggacggacat
     7441 gctcgagcag cgcgatcgca agctgctcga acgcgagcgc gacctcgaca agacccgcga
     7501 ggagctcgcc aaggcccacc aggagcaggt cgtggccctg gagcgggtca gcggcgtctc
     7561 ggccgaggag gcgaagggca tcctgctcga ggccgtccgc gacgaggccg agcacgatgc
     7621 ggtcaagctc gcccgctcga tcgagcgacg ggcccgcgag gaggccgacg agaaggcccg
     7681 cgacgtcgtc gtgaccgcga tccagcgggt cgccgcggac cacaccgccg agcacacggt
     7741 gaccgtcgtc catctcccca acgacgagat gaagggccgg atcatcggcc gcgaggggcg
     7801 caacatccgg gccctcgagc aggcgaccgg gatcgacctg atcatcgacg acacgcccga
     7861 gtcggtggtc ctgtcgggct tcgatccggt ccgtcgcgag gtcgcccgga tcgccctcac
     7921 gaagctgatc ggtgacggcc ggatccatcc gggccggatc gaggaagtcg ttgccaaggc
     7981 ccgcgcggag gtcgagctgg tcatccgcca ggccggcgag accgccgcgt acgagtgcgg
     8041 cgttcccggc ctgcccccgg agctgatcaa gttgctcggc cggctcaagt accggacgag
     8101 ctatggccag agcgtcctca accacgtcat cgagacgagc cggctcgctg cggtgatcgc
     8161 ggcggaggtc ggggcggaca tccagatcgc caagacgggt ggcctcctcc acgacatcgg
     8221 caaggccgtc gaccacgaga tcgaagggcc gcacgccgcg atcggggcga cgatcgccca
     8281 gcgcaacaac ctctcgccga aggtcatcaa cgcgatcggc gctcaccacc aggagatcga
     8341 gtacacctgc gtcgaggccc cgatcgtcca gatcgccgac gcgatcagcg cgtcgcgacc
     8401 cggcgcccgc ggcgagacga tggacgccta tgtcaagcgc ctcgaggacc tccaggcgat
     8461 cgccgacagc ttcgccggcg tcgagcgctc gttcgcggtc caggccggtc gcgaggtccg
     8521 gatcctcgtc cggcccgagg agatcgacga cctgaccgcg acccgcctcg cccgcgacat
     8581 cgtccgcaag atcgaggagc agctgacgta tccgggccag atcaaggtca cggtcatccg
     8641 cgagacgcga gcggtcgagt acgcgaagta gggaagtcgc gcgttcccgt tgacgcagaa
     8701 ccggaacagg agcactgtcc gcgcgtgacc ggtcgagggc tccagggacg acatctggcg
     8761 cgtctgctgc tgctcgccgc attcgcgctc gacctcatcc cggctttcct gttcgtctcc
     8821 gctccgaact tcatcgggcc gatgtccgcc gagccgccgt cccgactcgt gtggctgatc
     8881 ccggcagtcg gaatcgccct gaatctggcc gggctcgcct ggatggtccg gctctacctc
     8941 tccgattccg aggctcgacc gtccgcgttc cgcttcacgc accgctgacc gcggcacacg
     9001 gccctcttct cgtgcggccg cgtgaccgac gctcgtcggg tatcgtccgc gggtgactga
     9061 cgaaactccc gcggcgcccg gtagcggcgc cccgatcggg cggacacgcc tcggcaaccc
     9121 gaccaacggc aacgctccac ggccgcccgg c
//
LOCUS       DATAIW010000098        16609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_93934, whole genome shotgun sequence.
ACCESSION   DATAIW010000098 DATAIW010000000
VERSION     DATAIW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93934"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VHM48_03320"
     CDS             <1..250
                     /locus_tag="VHM48_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754465.1"
                     /translation="FYGIDTQVETELIAATHSVEEIRSFIGADSLGYLSIRGVLAALD
                     LPYDRFCFACFDGIYPEPVPYDAASRKFVLEEVVGARG"
     gene            463..1590
                     /gene="purM"
                     /locus_tag="VHM48_03325"
     CDS             463..1590
                     /gene="purM"
                     /locus_tag="VHM48_03325"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2754466.1"
                     /translation="MAEARSTYAAAGVDVEAGDRAVELMRASVERTRRPEVLGELGGF
                     GAAITIPAGFREPVLVSSTDGVGTKTAIAAALGRYDTIGRDLVAMCADDVVCSGAAPL
                     FFLDYVAVGRLEPEQIADLVGGVAAGCLEAGCALVGGETAEHPGLMQPGDFDLAGFCV
                     GVVERDDMLDGGAAHAGDAIVGLAASGLHANGYSLVRSQLAKWDLDLARPYQEQLILT
                     LGPEAAAGAMTAEADLALATLGDVLLTPTTIYAGALLRVRSTLRAAGHDLHGLAHITG
                     GGLPGNVPRVLTDTVTARLDPTTWRMPSIVRLIGALGGLDDDELRATFNGGLGMIAVV
                     SPDAVEATIGALAGEGITAMLVGEVVPADGGPRYIEGPLTR"
     gene            1587..3830
                     /gene="purH"
                     /locus_tag="VHM48_03330"
     CDS             1587..3830
                     /gene="purH"
                     /locus_tag="VHM48_03330"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX2754467.1"
                     /translation="MTGRIAVAVSGAGSNLRALHAAAARDELGGEIVLVVADRDCPAL
                     VWAGEQGIDTALVRGGADNVLAETLAGARPDVVVLAGYMRIVGPRVLAAYPGRILNTH
                     PSLLPAFAGAHAVRAALDHGAKVTGATVHLVDEDLDGGPIVLQESVPVLAGDDEATLH
                     GRIRAVEHRLLPRAVALLLAGALELPAAGGRTTTIDVERAESAVPMPRRALLSVSDKT
                     GLVALAAGLVGLGFELVSTGGTARALRDAGLPVTDVAAVTGAPEMLDGRVKTLHPRVH
                     AGILADRRLPDHRRQLAAAGIAPFELVVVNLYPFAAAAARPGISFDDLVEEIDIGGPS
                     MVRAAAKNHASVAIVTSPARYDAVLAAIHDDGRVPDGLRSALAIEAFRHTAAYDARIA
                     AELPDRMAAAGVELPNEPGMPGAADPYPPTLTIGLEKVETLRYGENPHQSAARYRRPG
                     RDGIDAGSLAGAEPPLQGKALSYNNVLDAAAAAAIARTLRGPACVIVKHTNPCGAAER
                     PTLREAWDAALAGDPVSAFGGVVALTRPVDRATAEGLTSLFLEVVVAPGFDEAARELL
                     AGKVNLRLIVDSAIGGERPPMAPDPLGAIRTAGGAVLVSTPDTSPDDPAGWQVATRRA
                     PTDPELGDLDLAWRLCRGVTSNAIVLVRAGRLVGFGSGQTSRVDAARQAVEKARAISG
                     AGVLAGAACASDAFYPFADAVEVCLEAGVTAYVQPGGSKNDAAVVAVADAAGAAMVLT
                     GVRHFRH"
     gene            complement(4246..4734)
                     /locus_tag="VHM48_03335"
     CDS             complement(4246..4734)
                     /locus_tag="VHM48_03335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2754468.1"
                     /translation="MVMIDIDHFKAFNDTYGHPAGDEALRSFARVVAGSIRASDVAAR
                     YGGEEFIVALRHVAIDEAQAIAEKLRASIEQMVVELGPGRSARITASFGVASTGPNLQ
                     DQKSLVSLADAALYRAKEGGRNRVELTPTAADVVVLNDAGRRRLGHIAAKHLEAVDPT
                     AG"
     gene            complement(4725..5186)
                     /locus_tag="VHM48_03340"
     CDS             complement(4725..5186)
                     /locus_tag="VHM48_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2754469.1"
                     /translation="MIGGRRSAGRRWVRPVASGAARASPSGSRTGPPADDGGGGEPWS
                     SPCRRSGEGGHAASGTMACVPMLALGKIVGVIHLESTSLNAFDPESVRVVTRVAENVG
                     LAMANARLMKTMEGQAMSDPLTGLRNTRSFDPTSSRSSKRPAVTSSPRAWS"
     gene            5271..6917
                     /gene="metG"
                     /locus_tag="VHM48_03345"
     CDS             5271..6917
                     /gene="metG"
                     /locus_tag="VHM48_03345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX2754470.1"
                     /translation="MPDDARPRFYLTTAIAYANSQPGVHTLYEVIGADAIARWHRMLG
                     DDTRFLTGTDEHSVNIAMRAAEDGKDTRAFVDEKVALFKAAEDALGIRPDRFIRTTDP
                     DHVRASQELVRRAYANGDIYVGTYEGWYCPNEGFRNATDVQETARGTICPNHPDVPLQ
                     WLTERNWFFRLSAYQERLERHFEENPEFVQPDYRRNEMLGFIRQGLEDFSISRAGSGW
                     GIPFPIREDGSSAQLPDGSWDPEAGTIYVWYDALINYVTGAGFPDDPEAFARYWPADL
                     HVIGKDILRFHTIFWPAMLWSAGLEAPRKVWAHGWLLVAGGERMSKSRGNFLDPGDFV
                     AAFGSDGARYVALREVPFDKDAEVSWDGFVRRYNADLANDFGNLVNRTVSMANRYLGG
                     ERPDPSPAAEADRTWSWTSRVPMLHAHLDDCLLHEALGTLWSVVSEANRFVDAEKPWE
                     LNKAARTGDEEAGGRLRTVLGDLVEACRLLGLAVAPFLPATAPRVLAQLGYAWPYGPD
                     GNGGPALLDELRWGAHAGEAGTLTTAEPLFPRLDVETEAF"
     gene            6955..7317
                     /locus_tag="VHM48_03350"
     CDS             6955..7317
                     /locus_tag="VHM48_03350"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754471.1"
                     /translation="MAHGEINHIEFPADDPERAMAFYSAVAGWEFSAMDGFDEYWVFR
                     SGEGYGGAVGKRGVSTGPVLRDYIEVNSIDDALAAGERTGGTVKEAKSEIAGMGWFAV
                     LIDPEGTEIGLFQSSALG"
     gene            7345..8139
                     /locus_tag="VHM48_03355"
     CDS             7345..8139
                     /locus_tag="VHM48_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2754472.1"
                     /translation="MRLVDSHCHLQADRFDDDVEAVIAAAREAGLERILVPGWNVWSS
                     AAAIALAERFPALDAAVGVHPHDAATVDDEAWAAIAASARDPRVVAIGETGLDYDRVF
                     SPILAQLANLRRNLVLAAETGKPAILHCRSRAGERDAQDALLAELRAFGADVPPVVVH
                     SFSGPLDYAEAMLALGAAISISGLAFRKGEDATADVVRLVPADRLLVETDSPFLAPPG
                     APRGRNAPEWVRVTAAWVAELRGMDLATLGDDLVATFDRMFARSGR"
     gene            8607..10217
                     /locus_tag="VHM48_03360"
     CDS             8607..10217
                     /locus_tag="VHM48_03360"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX2754473.1"
                     /translation="MANQYRLGAQPIVTLPANPTWGENPLGDSNWQFQLHAMRYVLDL
                     FTAARLTGDRAYRDRALFLLRDWVTDNPRHSAPSVWSWNDHTTALRAVVLACAADLAP
                     TTSWLHNALVVHGRTLADPTFYRNEGNHALNQDIGLLEVARVLKRSDWRRLAGSRINT
                     LIVGSVDAQGVTNEQSIGYEAFNYKRYREAEERMLAVGLEPSAAFARLDLMPQFLAQA
                     TLPNGTYEMIGDTSGWPADVIAGTPAESAATGGASGPKPTATITRYEAGYLFARSGWG
                     EHRPAADETFLSMKWGPAPIFHGHADGLELTLAAYGSRLLVDPGPFSYNGGIYRGFFK
                     GRSAHNVVTVDSSTWNRAAATRLLGYRQSARYVDIRLQGAGDRGVVHTRRMTYSRGLD
                     YILVEDRLTSASVHTYRQLWHFVEDAQPLVGGSSVWTERPRGNVLIRQLAGAPQVRIV
                     RGRTKPIQGWISYKQGQKLAAPVEEATQRGRSVRYLTLIVPAAGRPEATVSGLRLTSS
                     GYSVTITIGGRSERVTVSGTSVWIHELP"
     gene            10506..10820
                     /gene="groES"
                     /locus_tag="VHM48_03365"
     CDS             10506..10820
                     /gene="groES"
                     /locus_tag="VHM48_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2754474.1"
                     /translation="MATATASATKLRPLGDRVVIQPTAREEMTKSGIVLPDTVKEKPQ
                     KGLVLAVGPGRTLDDGKRESIDVKKGDTVLYAKYAGTEFKIDDEELLIVSQKDILAIV
                     ED"
     gene            10936..12564
                     /gene="groL"
                     /locus_tag="VHM48_03370"
     CDS             10936..12564
                     /gene="groL"
                     /locus_tag="VHM48_03370"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX2754475.1"
                     /translation="MAKQLIFDETARRSLKRGIDKLADAVKVTIGPKGRNVVLDKKFG
                     SPTITNDGVTIARDIELDDPFENMGAQLLKEVATKTDDVAGDGTTTAVVLGQALVSEG
                     LRVVTAGANPMVVKRGIEVAVAAVVAELEKHKVDVSSREQIAAVAAISAADPEVGEII
                     AEVMDKVGKDGVITVEEGQSLGLEKEYTEGMQFDRGYLSAYFVTNPDRMEAVLESPLI
                     LITDKKISSVQDMLPALEKAVQQGKPTLIIAEDIDGEALATLVVNKLRGTVSVLAVKA
                     PGFGDRRKEMLRDIATLTGGTVISEEVGRKLDSVTFEDFGSARRVVATKDDTTVIDGA
                     GSPDQIKARMSQIKAQIEDTTSDYDKEKLQERLAKLSGGVAVIKVGAATEVELKEKKH
                     RIEDALSTTRAAVEEGLVAGGGTALLQAIPALDNLKLEGDAQVGVDIVRKALEAPARQ
                     IADNAGQSGEVVINAIRNAKIGHGYDALRNEYGDMFKKGIVDARKVTRSALQNAASIA
                     AMVLTTETLITDIPEKAAAGAGGGHGHGGGDMDF"
     gene            12814..13317
                     /locus_tag="VHM48_03375"
     CDS             12814..13317
                     /locus_tag="VHM48_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754476.1"
                     /translation="MDIWLMVVQFVHILGGAAWLGGSIFANLVLLPYVFRQAPDRRRG
                     LIGVLILGPERVMIGAAVIGGLMGLVRGTFFGPIRSIDDLGSGYGLTWLGAVVVVLGV
                     FVVGGAVTSRAARRLLDDDALWVASADGRLGDAYATTAVRLKVGFRIELSGILVTLAL
                     MEVLRFS"
     gene            13465..14238
                     /locus_tag="VHM48_03380"
     CDS             13465..14238
                     /locus_tag="VHM48_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754477.1"
                     /translation="MIRRLALLPIAIVATLSLVLAACSSTPAAPALTDPKEIVTKGVT
                     SLVDVKTLEITATFTGSVKAAQLGTFDLSTIKLSAAVDSANKTAKFSLDAPTLLGTKI
                     DALMVGGSAYYKVAGMLAASLHATPDKYTKVAVPTASGDPAAAVTDVTKLVAQLQEGL
                     AKLPSPLTKASDEKCGDVDCYHVSTVVTAAQMKVLDATSTLDGDVTVDLWTRKSDYRP
                     AKIGLSIASATLGTFGMAIEIKYDVPVSVTAPPADQLAP"
     gene            14548..>16609
                     /locus_tag="VHM48_03385"
     CDS             14548..>16609
                     /locus_tag="VHM48_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2754478.1"
                     /translation="MPSPTRRPRSGRLADDAPSGEGTLFADVAEQPVDAVPQEPDWLR
                     DVPIPGDVPVELTADERRAREDEQKVTEKVRAAARAEEIVGRLNPEQARAVTATEGPL
                     LILAGAGSGKTRVLAHRIAYLVGVKNVRPWSILAVTFTNRAAGELRERIISLVGEPGR
                     DVQAGTFHSLCARVLRRDGEAIGIDRRFVVYDTEDQQSLMKQLLREEDLPLTGEFRPS
                     AVLGAISRAKNEMLDATFLAENAVNHRERTIAKLAVRYEQRLKKVGALDFDDLLLKAV
                     VLFDEAPDVLARYQDRWRYLHVDEYQDTNRPQYLWIRSLAAKYRNLAVVGDDDQSIYR
                     WRGADLRNILDFERDYPDATVVKLEQNYRSTQLILDAAHAVVSRNSERTDKKLWTNTS
                     GGAKIQRFEAYNEEEEAEWIARQVEGLVGGRGSVLTRRADDEDAGSLRARDIAVMYRM
                     NAQSRAIEESFLRYGIRYQLVGGTRFYSRREVKDALAYLRTLRSDTDSVSFERIINIP
                     ARAIGDKTIEVLRRASVQGGISTWEAIERASRGEVEGLGPRARGSIADFAVLIRRLRS
                     KVGVLNLPELLDETLEASGYRAMLADGSEDGEDRWANLLELRSVTTRYDDLEPEDALD
                     RLLEETALVADQDSYEGDADAVTLITLHAAKGLEFPVVFIAGLEEGVFPHSRALDDEK
                     ELEEE"
BASE COUNT         2495 a         6004 c         5683 g         2427 t
ORIGIN      
        1 cttctacggg atcgacaccc aggtcgagac cgagctgatc gcggcgaccc attcggtcga
       61 ggagatccgc tccttcatcg gcgcggattc gctcggctac ctctcgatcc ggggcgtcct
      121 cgccgccctc gacctgccgt acgaccgttt ctgcttcgcc tgcttcgacg gcatctaccc
      181 ggagccggtc ccgtacgacg ccgcgtcgcg caagttcgtc ctcgaagagg tcgtcggggc
      241 ccgtggctaa gaggtcgagc ggccgacttc gccattcacc atcccgagtg gtgcgtgcca
      301 cgtcgaatgc ggcggatcgg gggtcagtcc ggacccggtc agccgtcggc gccaaattcc
      361 tgccagcgtc catcctgtcg agccgccgcg ccggtgtttc gtactaccca gcgtggtgtc
      421 cgacagcgcc cgcggccggc gcctcgtggc tctggcccgc ccgtggctga agcgcgctcc
      481 acctacgccg ccgccggtgt cgacgtggag gccggcgacc gggcggtcga gctgatgcgg
      541 gcgtccgtcg agcggacccg gcggcccgag gtcctcggcg agctcggcgg tttcggggcc
      601 gcgatcacga tcccggcggg gttccgcgag ccggtcctgg tctcgtcgac cgacggcgtc
      661 ggcacgaaga ccgcgatcgc cgcagccctg gggcggtacg acacgatcgg ccgcgacctg
      721 gtcgcgatgt gcgccgacga cgtcgtctgc agcggcgcgg cgccgctctt cttcctcgac
      781 tacgtggcgg tcggccgtct cgaacccgaa cagatcgccg atctcgtcgg tggcgtggcg
      841 gcgggctgcc tcgaagccgg ctgcgcgctc gtcggtggcg agaccgccga acaccccggg
      901 ctcatgcagc cgggcgactt cgacctggcc gggttctgcg tcggcgtcgt cgagcgcgac
      961 gacatgctcg acggcggcgc ggcgcacgcc ggcgacgcga tcgtcggcct cgcggcgagc
     1021 ggcctccatg ccaacggcta ctcgctcgtc cgatcacagc tcgcgaaatg ggatctcgat
     1081 ctcgcgcggc cgtaccagga gcagctgatc ctgaccctcg ggcccgaggc ggcggccggc
     1141 gcgatgacgg ccgaggccga ccttgccctc gcgaccctcg gcgacgtcct gctgacgccg
     1201 accacgatct acgccggcgc cctgctccgg gtccgctcga ccctgcgggc ggccggccac
     1261 gaccttcacg gcctcgccca catcaccggc ggcggcctgc ccggcaacgt gccccgggtc
     1321 ctcacggaca ccgtgactgc ccggctcgat cccacgacgt ggcggatgcc gtcgatcgtc
     1381 cgcctcatcg gcgccctcgg cgggctcgat gacgacgagc tccgggcgac gttcaacggc
     1441 gggctcggga tgatcgccgt cgtgtcgccc gacgccgtgg aggcgaccat cggcgcgctg
     1501 gccggcgagg ggatcacggc catgctcgtc ggcgaggtcg tgccggccga tggcgggccg
     1561 cgctacatcg agggaccgct cacccgatga ccggccggat cgccgtcgcc gtatccggcg
     1621 cgggttcgaa cctgcgggcg ctccatgccg cggcggcgcg cgatgagctc gggggcgaga
     1681 tcgtcctggt cgtggccgat cgggactgcc ctgcactcgt gtgggcgggc gagcagggga
     1741 tcgatacggc gctcgtccgc ggcggtgccg acaatgtgct cgccgagacg ctcgcgggcg
     1801 cccggccgga cgtcgtggtg ctcgcgggct acatgcggat cgtcgggccg cgggtgctgg
     1861 cggcgtatcc cggccggatc ctgaacactc acccgagcct cttgccagcg ttcgccgggg
     1921 cgcacgcggt gcgcgctgcc ctcgaccacg gggcgaaggt caccggtgcc acggtccacc
     1981 tggtcgacga ggacctcgac ggcggcccga tcgtcctcca ggaatccgta cccgtcctcg
     2041 ccggcgacga tgaagcgacg ctccacggcc ggatccgagc cgtcgagcat cgcctcctgc
     2101 cgcgagccgt tgcgctcctc ctggcaggcg ccctcgagct gcccgcggcg ggcgggcgga
     2161 cgacgacgat cgacgtcgaa cgcgccgagt cggccgttcc gatgccgcgc cgggctctcc
     2221 tgtcggtctc cgacaagacc gggctcgtcg ccttggccgc cggcctcgtc ggcctcggct
     2281 tcgagctggt ctcgaccggc ggcaccgccc gcgccctccg cgacgccggc ctgcccgtga
     2341 ccgacgtcgc cgcggtgacg ggcgctccgg agatgctcga cggccgggtc aagacgctcc
     2401 atccccgggt ccacgccggc atcctggccg accgacgcct accggaccat cgccgccagc
     2461 tcgccgcagc ggggattgcc ccgttcgaac tcgtcgtcgt caacctctac ccgttcgcgg
     2521 ccgcggcggc tcgacccggg atctcgttcg acgacctcgt cgaggagatc gacatcggcg
     2581 gcccatcgat ggtccgggcg gccgccaaga accacgccag cgtcgcgatc gtgacgtcgc
     2641 cggcccgcta cgacgccgtg ctcgcggcga tccacgacga cggccgggtg ccggacggcc
     2701 tccgctccgc gctcgcgatc gaggcgttcc gccacaccgc cgcctacgac gcccggatcg
     2761 cggccgagct gcccgatcgg atggccgcgg ccggcgtcga gctcccgaat gagcccggca
     2821 tgcccggcgc cgccgatccg tatccgccga ccctgacgat cgggctcgag aaggtcgaga
     2881 cgctgcgcta cggcgagaac ccccatcaat cggcggcccg ctatcgccgg ccgggtcggg
     2941 acggcatcga cgcggggtcg ttggccggcg ccgagccgcc gctccagggc aaggccctga
     3001 gctacaacaa cgtcctcgac gcagcggcgg ccgcggcgat cgcgcggacc ctccgcgggc
     3061 cggcgtgcgt gatcgtcaag cacacgaacc cgtgcggtgc ggccgagcgc ccgacgctcc
     3121 gtgaggcctg ggacgcggcc cttgccggcg acccggtgtc ggcgttcgga ggggtggtcg
     3181 cgctcacccg cccggtcgac cgcgcgacgg ccgagggcct cacgagcctg ttcctcgagg
     3241 tcgtggtcgc ccccggcttc gacgaggcgg cccgcgagtt gcttgccgga aaggtcaatc
     3301 tgcggctcat cgtcgactcc gccatcggtg gcgagcgccc accgatggcg cccgatcccc
     3361 tcggcgcgat ccggacggcc ggcggcgcgg tcctggtcag cacgcccgac acgtcgcccg
     3421 acgatccggc cggctggcag gtggccaccc gtcgcgctcc gaccgacccg gagctcggcg
     3481 acctcgacct ggcgtggcgt ctctgtcgcg gcgtcacctc caacgcgatc gtcctcgtcc
     3541 gcgccggccg gctcgtcggg ttcgggtccg gccagacctc gcgcgtcgac gccgcgcgcc
     3601 aggcggtcga gaaggcccgc gcgatctccg gcgccggtgt ccttgcgggg gctgcctgcg
     3661 cctcggatgc gttctatcca tttgccgacg cggtcgaggt ctgcctcgag gcgggagtca
     3721 ccgcctatgt ccagcccggt ggctcgaaga acgacgcggc cgtcgtcgcg gtcgccgacg
     3781 ccgccggagc ggccatggtc ctgacgggcg tccggcattt tcgccattga gccagtcggc
     3841 cggggcggag gcggaaacgt cgttccgacc gccctgcgct gcgtcatcgt cggttgcagc
     3901 gatggggcat cgaaacgcgg gtcacgggct caccgatgga ggcgctgggc tgggtccgcg
     3961 gcggcgaacc gttcgacctc gtcctgagcg acctgcctgc tgatcaccac ctcttgactg
     4021 acccgccatc atgatcggcg ccgggggacc ggcatcatcg tgggcgggat ggcgaccggg
     4081 ggaagccgtc ccgccctcct gattccagcg gtgaggccga cgaccagagc gaagccgatt
     4141 ctggcgagtc ctgcagcccg tctccagacc agaacatgag gacagccaga tgggcttgct
     4201 gtgggaggtc gtcccggcgg tcggagggcg cgtcgggacc ggcggtcagc ccgcggtcgg
     4261 atcgacggct tcgaggtgct tggcagcgat gtgcccgagg cgtcggcggc cggcatcatt
     4321 gaggacgacg acgtcggccg cggtgggagt gagttcgacg cggttccggc cgccctcctt
     4381 ggcccgatac agggccgcgt cggcgaggga cacgagtgac ttctggtcct gcaggtttgg
     4441 tccggtggaa gcgaccccga acgaagcggt gatccgggca gatcgacccg ggccgagttc
     4501 caccaccatc tgctcgatcg atgcccggag cttctcggcg atggcctggg cctcgtcgat
     4561 cgcgacatgg cggagggcga cgatgaactc ttcgccgccg taccgggccg cgacgtcgga
     4621 tgcgcggatg gatccggcga cgacgcgggc gaacgatcgc agcgcctcgt cgccggccgg
     4681 atggccgtag gtgtcgttga aggccttgaa gtggtcgatg tcgatcatga ccacgctcgt
     4741 gggctgctcg tcacggcggg ccgcttcgag ctcctgctcg aggtagggtc gaaggaacgg
     4801 gtattgcgga gtccggtcag cggatcggac atggcctgac cttccatcgt cttcatcagc
     4861 cgggcattgg ccatcgccaa tccgacgttc tctgcgacgc gggtcaccac tcggacggac
     4921 tccggatcga atgcgttcag cgacgtcgat tcgaggtgga tcacgccgac gatcttgccg
     4981 agggcaagca tcggtacgca ggccatggtc ccgctggcag cgtggccgcc ctcaccactt
     5041 cggcgacacg gacttgacca cggctcgccg cctccgccat catcggctgg tggaccagtt
     5101 cgggagcctg atggcgatgc ccgagcggcc ccgctcgcga caggcctgac ccagcgcctg
     5161 ccggccgagc gtcggccgcc gatcatccgg cgcgctccgc gcgcctgcgc ccggcctgcc
     5221 cagggctttc ggcatcagcg ccgacggccc agcgccaccc cgaggatccg atgcccgacg
     5281 atgcccgccc gcgcttctac ctgacgactg cgatcgccta cgccaacagc cagcccggcg
     5341 tccacaccct gtacgaggtg atcggcgccg atgccatcgc ccggtggcac cggatgctcg
     5401 gcgacgacac ccgcttcctg accggaaccg acgagcactc ggtcaacatc gcgatgcggg
     5461 cggccgagga cggcaaggac acgcgggcgt tcgtcgacga gaaggtcgcc ctgttcaagg
     5521 cagccgagga cgccctcggg atccgccccg accggttcat ccggacgacc gatccggacc
     5581 acgtccgggc ctcccaggag cttgtccggc gggcgtacgc gaacggcgac atctatgtcg
     5641 ggacgtacga gggctggtat tgcccgaatg aaggcttccg caacgcgacc gacgtccagg
     5701 agacggcccg cggcacgatc tgcccgaacc atccggacgt gccgctccag tggctgaccg
     5761 agcgcaactg gttcttccgg ctgtcggcct accaggaacg cctcgagcgc catttcgagg
     5821 agaatccgga gttcgtccag cccgactacc gccggaacga gatgctcggc ttcatccgcc
     5881 agggcctcga ggacttctcg atcagccggg cgggctccgg ctggggcatc ccgttcccga
     5941 tccgcgagga cggctcgtcg gcgcagctcc cggacggctc gtgggacccg gaggccggca
     6001 cgatctacgt ctggtacgac gccctgatca actacgtcac cggagccggc tttcccgacg
     6061 atcccgaggc ctttgcccgc tactggccgg ccgacctcca cgtcatcggc aaggacatcc
     6121 tccggttcca cacgatcttc tggccggcga tgctgtggag cgccgggctc gaggcgccgc
     6181 gcaaggtctg ggcccacggc tggctgctcg ttgccggcgg cgagcggatg agcaagagcc
     6241 ggggcaactt cctcgacccg ggcgacttcg tggcggcgtt cggcagcgac ggcgcccgct
     6301 acgtcgccct ccgcgaggtg ccgttcgaca aggacgccga ggtgagctgg gacggcttcg
     6361 tccggcgcta caacgccgat ctcgccaacg acttcgggaa cctcgtcaac cggacggtgt
     6421 cgatggccaa ccgctacctc ggcggcgagc gacccgaccc gagcccggcc gccgaggccg
     6481 atcgaacatg gagctggacc agccgcgtgc cgatgctcca cgcccatctc gacgattgcc
     6541 tcctccacga agccctcgga accctgtggt ccgtcgtgtc ggaggccaac cggttcgtcg
     6601 acgccgagaa gccatgggag ctgaacaagg cggccaggac cggcgacgag gaggccggcg
     6661 gacggctgcg gaccgtcctc ggcgacctcg tcgaggcctg ccggctgctc ggactcgccg
     6721 tggccccgtt cctgccggcg accgcgccac gtgtcctcgc ccagctcggc tacgcctggc
     6781 cgtacggccc ggatggcaac ggtgggccgg cgctgctcga cgagctgcgc tggggcgccc
     6841 atgccggcga ggcggggacg ttgacgaccg ccgaaccact cttcccgcgc ctcgatgtcg
     6901 aaaccgaggc attctgatcg tcgatccgac gaaccccacc cgaggaggca cctgatggcc
     6961 cacggcgaga tcaaccacat cgagttcccg gccgacgatc cggagcgggc gatggccttc
     7021 tacagcgcgg tcgccggctg ggagttctcg gcgatggacg gcttcgacga gtactgggtc
     7081 ttccggtccg gcgagggcta cggcggcgcg gtcggcaagc gcggtgtgtc gaccggcccg
     7141 gtcctgcgcg actacatcga ggtcaactcg atcgacgacg cgcttgcggc aggcgagcgg
     7201 accggcggga cggtcaagga agcaaagtcg gagatcgccg gcatgggctg gttcgccgtc
     7261 ctcatcgatc ccgagggaac cgagatcggc ctgttccaga gcagcgcgct cggctgagcc
     7321 cgacgcagac cggggagcga ggcgatgcgc ctcgtcgaca gccactgcca cctccaggcg
     7381 gaccgcttcg acgacgatgt cgaggcggtc atcgccgcgg cacgcgaggc cggcctcgag
     7441 cggatcctcg tccccggctg gaacgtgtgg tcgtcggccg ccgcgatcgc tctcgccgag
     7501 cggttcccgg cgctggatgc cgccgtcggc gtccatcccc acgacgcggc cacggtcgac
     7561 gacgaggcgt gggccgcgat cgcggccagc gctcgggatc cgcgcgtcgt cgcgatcggc
     7621 gagaccggcc tcgactacga ccgcgtcttc tcgccgatcc tggcccagct ggcgaacctg
     7681 cgccggaacc tcgtcctggc tgccgagacc ggcaagccgg cgatccttca ctgtcgatcg
     7741 agggcagggg agcgcgacgc ccaggacgcc ctcctcgccg agttgcgggc gttcggcgct
     7801 gacgtccccc cggtcgtcgt ccattccttc agcggcccgc tcgactacgc cgaggccatg
     7861 ctcgcgctcg gcgcggcgat cagcatctcg gggctcgcct tccgcaaggg cgaggacgcg
     7921 accgcggacg tcgtccggct ggttccggcc gatcgactcc tcgtcgagac cgactcgccc
     7981 ttcctcgccc cgcccggcgc gccgcgcggc cgcaacgcgc ccgagtgggt ccgtgtgacc
     8041 gcggcctggg tcgccgagct gcgcggcatg gatctcgcga cgctcgggga tgacctcgtc
     8101 gccacgttcg atcgcatgtt cgctcgctct ggccgctgac gcgccgacgg cgcccgaggc
     8161 tcgcgcgaag cggccgtccg ctcggtggct tcgatgcctg gacgggcgcg aggcgctacc
     8221 gttcggtcat ccgggcgcct gccgcgcccg ccgtcacctg ccttgctctg agggggaccg
     8281 gaactttgcc gaaccggccg atggccgctc cacgggatcg cgggcttgcc tcgtggcgat
     8341 ggccgatggc gatcctcgcg atcgtgctga ttctcgtttc gatgccgctc gccggttggg
     8401 gacttgcggc cggacccagt cctgccggct cgggggtcgc cgcggcgtca cccggatcgt
     8461 cgcacgtacc gtcgccacct gagtcgtcac ccggaccggc gaggagcccc tccgcgccag
     8521 cgtcggcgcc tccgatcgtc gcggacgccg agctcagccc ggactgcctc aactcgagcg
     8581 acccaatcgc ccgggccgac gagctgatgg ccaatcaata cagactcggg gcgcagccga
     8641 tcgtcactct cccggcgaac ccgacctggg gcgagaaccc gctcggtgac tcgaactggc
     8701 agttccagct gcacgcgatg cgctacgtcc tcgacctgtt cacggctgcc cgcctgaccg
     8761 gagacagggc gtaccgggac cgggcgctgt tcctgctccg cgactgggtg accgacaatc
     8821 cgcgccattc ggcgccgtcg gtctggtcgt ggaatgacca tacgaccgcg ctccgggcgg
     8881 tcgtcctcgc ctgcgcggcg gacctggccc cgacgacgag ctggctccac aacgccctgg
     8941 tcgtccatgg ccggaccctg gcggacccga ccttctaccg caacgaaggc aaccacgcgc
     9001 tgaaccagga catcggcctg ctcgaggtgg cgcgggtcct gaagcggtcc gactggcgca
     9061 ggctggccgg cagccggatc aacaccctga tcgttggcag cgtggacgcg caaggggtca
     9121 ccaatgagca gtcgatcggg tatgaggcct tcaactacaa gcgctaccgc gaggcggaag
     9181 agcggatgct cgcggtcggg ctcgagccga gcgccgcctt cgcccggctg gacctgatgc
     9241 cccaattcct ggcccaggcc acgctcccga acgggacgta cgagatgatc ggcgacacga
     9301 gcggctggcc ggccgatgtc atcgccggaa cccccgccga gtctgcggca accggcggcg
     9361 cctccggccc gaaaccgacc gcgaccatca cccgctacga ggcgggctac ctgttcgcga
     9421 gatccggctg gggcgagcat cgcccggcgg ccgatgagac gttcctgtcg atgaagtggg
     9481 gcccggctcc gatcttccac ggccacgccg acggactcga gctaacgctc gccgcgtacg
     9541 gttcgcgcct cctcgtcgat ccagggccat tctcctacaa cggcggcatc taccgaggat
     9601 tcttcaaagg ccggtcggcg cacaacgtcg tcaccgtcga cagctccacc tggaaccggg
     9661 cggcggcgac gcgcctgctc ggataccgac agtcggcccg atacgtcgac atccgcctcc
     9721 agggggcggg tgatcgaggc gtcgtccaca cccggcgcat gacgtactcg cggggactcg
     9781 actacatcct cgtcgaggat cggctgacct cggccagcgt ccacacgtat cgccagctct
     9841 ggcacttcgt cgaggatgct cagcctctcg tcggcggttc gagcgtctgg acggagcgac
     9901 cgcgggggaa cgtccttatc cgccagctcg ccggcgcacc gcaggtccgg atcgtcaggg
     9961 gccggacgaa gcccatccag ggttggatct cctacaagca gggccagaag ctcgccgcgc
    10021 cggtcgagga agcgacccag cgcgggcgca gcgtccgcta cctcaccctg atcgtgcccg
    10081 ccgccggcag accggaggcg accgtcagtg gcctgcgcct caccagcagc ggctactcgg
    10141 tcacgatcac gatcggcggt cgctccgagc gcgtcacggt cagcggaacg tcggtctgga
    10201 tccacgagct gccctgaggt ccgactcccg gatcggacgc gcggcgcgac cgcgatcggg
    10261 gttgacagcc cggcgagccc gcggctacag tattagcact ctcacatcga gagtgctagc
    10321 gcggtccgac cgaggacctg gagggggcca atccgtcgac tgacggacgc ccgagaggtt
    10381 cggtctcgac ccgtcgcggc acttcaccac atcgccgggg acgtgatcga cccatgccga
    10441 tccggttcgc ccccgcccat cgagaccgac gtcggccacc accgccgtcc caggaggaat
    10501 gcacgttggc caccgccacc gcttccgcga cgaagctccg cccgctgggc gaccgcgtcg
    10561 tgatccagcc gaccgcacgt gaggagatga ccaagagcgg catcgtcctt ccggacaccg
    10621 tcaaggagaa gccccagaag ggcctcgtcc tggccgtggg cccgggccgc accctcgacg
    10681 acggcaagcg cgagagcatc gacgtcaaga agggcgacac ggtcctctac gccaagtatg
    10741 ccgggaccga gttcaagatc gacgacgagg agctcctcat cgtcagccag aaggacatcc
    10801 tcgcgatcgt cgaggactga atcctccggc cgatgcgctc caccgagcgc gctcggccgt
    10861 cggagggcga gtgatcgctc accgctgagg ttgccaactc gacccccacg cacgtctcca
    10921 ggagggacat ccaaattggc taagcaactg atcttcgacg agaccgctcg tcgcagcctc
    10981 aagcggggca tcgacaagct cgccgacgcc gtcaaggtga cgatcggccc gaagggccga
    11041 aacgtcgtcc tcgacaagaa gttcggctcc ccgaccatca ccaacgacgg cgtgacgatc
    11101 gctcgcgata tcgagctcga cgacccgttc gagaacatgg gcgcccagct cctcaaggaa
    11161 gtcgccacca agaccgacga tgtcgccggc gacggcacga ccaccgcggt cgtcctcggc
    11221 caggcgctcg tctccgaggg tctccgtgtc gtcaccgccg gggccaaccc gatggtcgtc
    11281 aagcgcggca tcgaggtcgc cgtcgcggcc gtcgtggccg agctcgagaa gcacaaggtc
    11341 gacgtctcgt cgcgcgagca gatcgcggcc gtcgcggcga tctcggccgc ggatccggaa
    11401 gtcggcgaga tcatcgccga ggtgatggac aaggtcggca aggacggcgt catcaccgtc
    11461 gaggaagggc agtcgctcgg cctcgagaag gaatacaccg agggcatgca gttcgaccgg
    11521 ggctacctct cggcgtactt cgtcaccaac cccgatcgga tggaggcggt tctcgagagc
    11581 ccgctcatcc tgatcaccga caagaagatc tccagcgtcc aggacatgct cccggcgctc
    11641 gagaaggccg tccagcaggg caagccgacc ctgatcatcg ccgaggacat cgacggtgag
    11701 gccctcgcga cgctcgtcgt gaacaagctc cgtggcaccg tctcggtcct ggccgtcaag
    11761 gccccgggct tcggcgatcg ccgcaaggag atgctccgcg acatcgccac actgaccggt
    11821 ggcaccgtca tcagcgagga agtcggccgc aagctcgact ccgtcacgtt cgaggacttc
    11881 ggctcggctc gccgcgtcgt cgcgaccaag gacgacacga ccgtcatcga cggcgccggc
    11941 tcgcccgacc agatcaaggc ccggatgagt cagatcaagg cccagatcga ggacacgacc
    12001 tccgactacg acaaggagaa gctccaggag cgtctcgcca agctgtcggg tggcgttgcc
    12061 gtgatcaagg tcggcgcggc caccgaggtc gagctcaagg agaagaagca ccgcatcgag
    12121 gatgcgctct cgacgacccg tgcggccgtc gaggagggcc tcgtcgccgg cggcggcacc
    12181 gcgctcctgc aggccatccc ggccctcgac aacctcaagc tcgagggtga cgcccaggtc
    12241 ggcgtcgaca tcgtccgcaa ggctctcgag gccccggccc gccagatcgc cgacaacgcc
    12301 ggccagtccg gcgaggtcgt gatcaacgcg attcgcaacg ccaagatcgg ccacggctac
    12361 gacgccctcc gcaacgagta cggcgacatg ttcaagaagg ggatcgtgga cgcccgcaag
    12421 gtgacccggt ccgccctcca gaacgcggcc tcgatcgcgg cgatggtcct gacgacggag
    12481 acgttgatca cggacatccc cgagaaggca gcggctggcg ccggcggtgg ccacggccac
    12541 ggcggcggag acatggactt ctagttctcg agtcggagca acgctcgaaa gcccctcccg
    12601 gcgacgggag gggctttcgt ctgtcaggtc ggactcacgc acgaagcagg ctcacggccg
    12661 cgcccggctt cggagcgcgc cggacctgcc gatctgtcag gctgcgtcag gcggtgctcc
    12721 cgctgatcct ggcgagttcc tgctccgtgc gacgcccttc cggagcaagg ccgacgggat
    12781 ccgcccagcg cagcgtcgtg atcggaggcc cggatggaca tctggctcat ggtggtccag
    12841 ttcgtccata tcctcggagg ggccgcctgg ctgggcgggt cgatcttcgc gaatctcgtg
    12901 ctgctgccct acgtgttccg ccaggcgccg gaccgccgac gcgggctgat cggcgtcctg
    12961 atcctcgggc ccgagcgggt gatgatcgga gcggccgtga tcggcggcct gatggggctc
    13021 gttcgcggga cgttcttcgg cccgatccgc tcgatcgacg acctcggctc tggctacggc
    13081 ctgacgtggc tcggggcggt ggtcgtcgtg ctgggggtct tcgtggtcgg cggcgcggtg
    13141 acgagtcgcg ccgctcgccg tctgctggac gacgacgcgc tctgggtggc ctcggcggac
    13201 ggccggctgg gcgacgccta cgccacgacc gctgtccgtc tcaaggtcgg cttccggatc
    13261 gagctgagcg ggattctcgt caccctggcc ctcatggagg tgctccgctt ctcctgacga
    13321 ggcgcgacga acccggacgt tcgggcgcct gacgacggcg tgcggcgcgc cgtcaggctg
    13381 aaacggtacg ttcggccctt tgacacggac gtgggcgccg gggcacgctg tcgcccgttc
    13441 atccaaccca ttcggggaga attcatgatc cgccgcctcg cactcctgcc gatcgccatc
    13501 gtggcgaccc tctcgctcgt tctcgccgcc tgcagctcga cgccggccgc gccggccctg
    13561 accgatccga aggagatcgt gaccaagggc gtcacctccc tcgtggacgt caagacgctc
    13621 gagatcacgg ccaccttcac cggtagcgtc aaggccgccc agctgggcac cttcgacctc
    13681 tcgacgatca agctgagcgc cgcggtcgat tccgccaaca agaccgccaa gttcagcctc
    13741 gacgcgccga ccctgctcgg cacgaagatc gatgccctga tggtcggcgg ctcggcctac
    13801 tacaaggtcg cggggatgct tgcggcgtcg ctccatgcca ccccggacaa gtacaccaag
    13861 gtcgccgtcc cgaccgcatc cggtgatccc gccgcggccg tgaccgacgt cacgaagctt
    13921 gtggctcagc tccaggaagg gctcgccaag ctgccgtcgc cgctgacgaa ggcctccgac
    13981 gagaagtgcg gcgacgtcga ttgctaccac gtgtccaccg tcgtgaccgc ggcccagatg
    14041 aaggtgctcg atgcgacctc gaccctcgac ggtgatgtca cggtcgatct ctggacccgc
    14101 aagagcgact atcgtccggc gaagatcggg ctctcgatcg cgtccgcgac cctcgggacg
    14161 ttcggcatgg cgatcgagat caagtacgac gtcccggtgt ccgtcaccgc cccaccggcc
    14221 gaccagctcg ccccgtagcg gcgtcccgtt cggaacccag cagcgaggcc cgggtccacg
    14281 acccgggcct cgcgacgccc ggccgattct cgcggggcgt gcacatgcga cgcgctcgtc
    14341 acgcaacggc gggcgtcggg tcgcggattc acccatgcag tgaacgatcg gccggaatcg
    14401 ggctgaatcg ggacttcgcg acccggattc gcctcctcgg gatgctctgt tggtccgatc
    14461 tgctcacggg gcgagaacgg gccgggtccc cgcggctctg gcggcgtgga tgcggcccgg
    14521 cgaaggcccc cgacctacaa tgacgcggtg ccatccccga cccgtcgacc gcgctccgga
    14581 cgcctcgccg acgatgcccc gagcggtgag ggaacgctgt tcgcggacgt cgcggagcag
    14641 cccgtcgacg ccgtcccgca ggagcccgac tggctgcgcg acgtgccgat cccgggcgac
    14701 gtcccggtcg agctgaccgc cgacgagcgg cgcgcccgcg aggacgagca gaaggtcacc
    14761 gagaaggtcc gggctgcggc gcgcgccgag gagatcgtcg gccggctcaa tcccgaacag
    14821 gcccgcgccg tcaccgccac cgaggggccg ctcctgatcc tcgccggcgc gggctcgggc
    14881 aagacccgcg tcctcgccca caggatcgcg tatctcgtcg gggtgaagaa cgtccggccg
    14941 tggtcgatcc tcgcggtcac cttcacgaac cgcgccgccg gggagctgcg cgagcggatc
    15001 atcagcctcg tcggcgagcc tggccgcgac gtccaggcgg ggaccttcca ctcgctctgt
    15061 gcccgcgtcc tccgtcgcga cggcgaggcg atcgggatcg accgtcgttt tgtggtgtat
    15121 gacaccgagg atcagcagag cctgatgaag cagctcctcc gcgaggagga cctgcccctg
    15181 accggcgagt tccggccgag cgccgtgctc ggggcgatca gccgggcaaa gaacgagatg
    15241 ctcgacgcga cgttcctcgc agagaacgcc gtcaaccacc gcgagcggac gatcgccaag
    15301 cttgcggtcc gctacgagca gcgcctcaag aaggtcggcg ccctcgactt cgacgacctg
    15361 ctcctcaagg ccgtcgtcct gttcgacgag gcgcccgacg tcctcgcccg ttaccaggat
    15421 cgctggcgct atctccacgt cgacgagtac caggacacga accgtccgca atacctctgg
    15481 atccggtcgc tggccgccaa gtaccgcaac ctcgcggtcg tcggcgacga cgaccagtcg
    15541 atctaccgct ggcgcggggc ggacctccgc aacatcctcg acttcgagcg cgactacccc
    15601 gatgccacgg tcgtgaagct cgagcagaac taccgctcga cccagctgat cctcgacgcc
    15661 gcccatgccg tcgtgtcgcg caacagcgag cggaccgaca agaagctctg gaccaacacg
    15721 agcggcgggg cgaagatcca gcgcttcgag gcctacaacg aggaggagga ggcggaatgg
    15781 atcgcccgcc aggtcgaggg cctggtcggc ggccgcgggt ccgtcctcac gcgccgcgcc
    15841 gacgacgagg acgcgggctc gctccgtgcc cgcgacatcg cggtgatgta ccggatgaac
    15901 gcccagtccc gcgcgatcga ggagtcgttc ctgcgctacg ggatccgcta ccagctcgtc
    15961 ggcgggacgc ggttctacag ccggcgcgag gtcaaggacg cgctcgccta cctgcggacg
    16021 ctccggagcg acaccgacag cgtcagcttc gagcggatca tcaacatccc ggcgcgggcg
    16081 atcggggaca agacgatcga ggtcctccgc agggcgtcgg tccagggcgg gatctccacg
    16141 tgggaggcga tcgagcgggc gtcccgcggc gaggtcgagg ggctgggacc gcgggcccgt
    16201 gggtcgatcg ccgatttcgc cgtgctgatc cggcgcctcc gctcgaaggt cggcgtcctg
    16261 aacctgccgg agctgctcga cgagacgctc gaggcgtcgg gctaccgggc gatgctcgcc
    16321 gatggctcgg aggacggcga ggaccgctgg gcgaacctgc tcgagctgcg ctcggtcacg
    16381 acccgctacg acgacctcga gcccgaggac gccctcgacc gcctgctcga ggagacggcc
    16441 ctcgtcgccg accaggattc gtacgagggc gacgccgacg cggtgacgtt gatcacgctc
    16501 cacgccgcga agggcctcga attcccggtc gtcttcatcg ccgggctcga ggagggcgtc
    16561 tttccccaca gccgggcgct cgacgacgag aaggagctcg aggaggagc
//
LOCUS       DATAIW010000099         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_153664, whole genome shotgun sequence.
ACCESSION   DATAIW010000099 DATAIW010000000
VERSION     DATAIW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153664"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..230)
                     /locus_tag="VHM48_03390"
     CDS             complement(12..230)
                     /locus_tag="VHM48_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX2754479.1"
                     /translation="MVSSTHDRIVLDSAKVGERVREGEILEVIEAEYGTRYRVAWEDG
                     HESTIRPAAGSAHVIPAPKSTKVKGRRR"
     gene            complement(416..604)
                     /locus_tag="VHM48_03395"
     CDS             complement(416..604)
                     /locus_tag="VHM48_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754480.1"
                     /translation="MNRTFLLVSGAICWTIAGADAAVHLLGGDLLAPAAMAFAFALWV
                     GLRRLQVRRLQVRQRQAA"
     gene            1104..3377
                     /locus_tag="VHM48_03400"
     CDS             1104..3377
                     /locus_tag="VHM48_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2754481.1"
                     /translation="MLAANLRNKLTRPWFAFLCAGLVAIAAYFAIPDWQDGLYAGVGL
                     ASSVAIVIGWKRYRASQRAWLAIALGLALYSFGDIAYSIIAALTASEPFPSAADGFYL
                     AGEVLIVIGVGRLAGTIGRGLYRPALLDAALVATAGAFIAWPLVLDPISAQVTDPLSG
                     LIALSYPIVDLVLVGVLTRHFLYPGKKTLASLLLAAGVGVWLVVDLVYAVMSLTDGYA
                     SGDPIDLGWLLAYTLVGAAGLHPSMAALVRTSETHEVTVSNGRLVLIGASVTIPVFVF
                     LLHGPMVHAGDYLAFGLGSVIIGVLGSVRLLGSLHSSRVLLAAQDSLQGELDRRARTD
                     HLTGLSNRQSIVERLASDLASGAPTALMFLDLDDFKRVNDAFGHPAGDAFLREVAERI
                     RSVIRNPDDVARLGGDEFAVVVSRCVDEEAAVAVARRIYDAFAADVRLDGHHFRIQAS
                     IGIVWCGGSDLTADEVLSRADIAMYRAKERGDGSYALFEQAMHDSALDRAQLRSDLEG
                     AVGRGEITPWFQPIFDVTTRRLVAVEALARWNHPARGLVAPDDFVPIAELSGSIAEID
                     RLVLRVAAGHVADWIDMIGAPLQLHVNITPREAADPATVDAIAAALDEAKLPANCLVV
                     EVVETALIDETAVAPVLAELKALGVLLSIDDFGSRYAVLTQLGRLPIDIVKLDRSLVA
                     GVETAEGFRLLEGILRLAQSLRLDTVAEGVESTAVMPILRRLGCTAAQGYSLGRPMPA
                     ADFERRLVKWSRETLSA"
     gene            3682..>4464
                     /locus_tag="VHM48_03405"
     CDS             3682..>4464
                     /locus_tag="VHM48_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754482.1"
                     /translation="MPEAPAALRSAFSALRQALANDGIRRLGISWTLGVAADSALLVV
                     TLVTVFNGGGFVAAAVLGAVRMIPAVVAGMLAGTMLERFRGDRILVVLGLVRAASAGL
                     TVFAMVTAGPTLADHHVTVVLLFVAAAVAAAAGAPVRPTQVTLMPTIARSPAELVTAN
                     TAWSTGEGLGAFGGPFVAGILMGQQLHIVVAVVAAVAFIVTAVVGAGLRFEQAADASG
                     GGRHTTNGIRLMDGVRALRARPVLAWSMLGTFGQVVTRGLLSA"
BASE COUNT          560 a         1581 c         1534 g          789 t
ORIGIN      
        1 ggtggagctc gctagcggcg ccgccccttg accttcgtcg acttcggggc cgggatcacg
       61 tgggcgctgc ccgccgcggg gcggatcgtg ctttcgtgcc cgtcctccca ggcgacccga
      121 tagcgggtcc cgtactcggc ctcgatcacc tcgaggatct cgccttcccg gacgcgttcg
      181 cccaccttgg ccgagtcgag aacgatccgg tcgtgggtgc tgctgaccat ggcgtcgacc
      241 tccgagtgag ggccgatccg ccgggcgtgc cgattcgtcc gccgtcccct cggccgtctg
      301 ccatcgtggc atcgggggcc ctggatccgg cagtgccagt cggcccgtca ccggcgggac
      361 gccggcgaag gtcgagggcg tcgccggatg gactcccgag cttcgatcgg ccgcgtcagg
      421 ccgcctggcg ctgcctgacc tggagccgcc tcacctggag ccgccgcagg ccgacccaca
      481 gggcgaacgc gaacgccatg gccgccgggg cgagcaggtc accgccgaga agatggacgg
      541 cggcgtccgc acccgcgatc gtccagcaga tcgcaccaga cacgagaagg aaggttcgat
      601 tcatccggat cccctggctt gggccgcatg ggtgtgcccc gtcggcctgg ccaacatagc
      661 cgatcgtcct gcccgttcaa tacccaatgg tcctatctgg ttcgcttctg caccggaacg
      721 ttggcgacgt cctcaccagg ctccggacgc ggcacgggcg cccgaccccg agggcccggc
      781 ggccggcggg catggtctcg cgaccttcgg atctcgtcag ctggccggat tcggcaatat
      841 cgggctcatc agctggacgt cgttgtcgtg cgggtcggcg agcgtggcga tggacacgtc
      901 cgacacgtcc ggtagtcctt cgcggcgatc ccgatcgatc cggcccgcca ccggtaccgg
      961 gggctcgtgc gtcctcggta gcccgatcga ggggtgacgc tgctccaatg gtccgtgcaa
     1021 gcatcgtcgc cacaggcggg tggctgtcgc gcgggtcacg ggacccgcgc tcgttgccgt
     1081 cttccgtcag gagtcgcaaa cgcgtgctgg ccgcgaatct tcgcaacaag ctcactcggc
     1141 cgtggttcgc cttcctttgc gcgggcctcg tcgcgatcgc ggcctatttc gcaatccctg
     1201 actggcagga cgggctgtac gcgggcgtcg ggctcgcgtc atccgtcgcg atcgtcatcg
     1261 gctggaaacg ataccgggca tcgcaacggg cctggctggc gatcgccctc ggcctcgccc
     1321 tctactcgtt cggtgacatc gcctactcga tcatcgccgc gctgaccgca tccgagccgt
     1381 tcccgtcggc cgccgacggg ttctacctcg cgggcgaggt cctcatcgtc atcggggtcg
     1441 gacggctggc gggcacgatc gggcgtggcc tgtacaggcc ggccctgctc gacgcggcgc
     1501 tcgttgcgac ggcgggggct ttcatcgcct ggccgctcgt cctcgacccg atctccgctc
     1561 aggtgacgga cccgctgtcc ggattgatcg cgctttcgta cccgatcgtc gacctggtcc
     1621 tcgtcggggt gctcacgcgc cacttcctgt atccgggcaa gaagaccttg gccagcttgc
     1681 tgctcgctgc cggggtcggc gtctggctgg tcgtcgacct ggtctacgcc gtgatgtcgc
     1741 tgaccgacgg gtacgcctcg ggcgatccga tcgacctcgg ctggctcctt gcctacacgc
     1801 tcgtcggtgc cgcgggcctc catccttcga tggctgcgct cgtccggacg tccgagacgc
     1861 acgaagtcac cgtgtcgaac ggtcgtctcg tgctcatcgg cgcctccgtg accatccccg
     1921 tgttcgtctt cctgctgcac gggccgatgg tccacgccgg cgactatctt gcgttcgggc
     1981 tcggctccgt catcatcggt gtgctcgggt cagtgcgtct gctcgggtcg ctccattcgt
     2041 cgcgagtcct cctcgctgcg caggattccc tccagggcga gctcgaccgc cgagcccgca
     2101 ccgaccatct gaccgggctg tcgaaccgcc aatccatcgt cgagcgactg gccagcgacc
     2161 tcgcgagcgg cgctcccacc gccctgatgt tcctcgatct cgacgacttc aagcgggtca
     2221 atgacgcctt cggacacccc gcgggcgatg ccttccttcg agaggtggcc gagcgaatcc
     2281 gctcggtcat ccggaacccg gacgacgtcg cgcggctggg gggcgacgaa ttcgccgtcg
     2341 tcgtgagccg ctgcgttgat gaggaagccg cggttgccgt cgcgcggcgg atctacgacg
     2401 cgtttgccgc ggacgtgcgg ctcgacggcc accacttccg gatccaggcg tcgatcggga
     2461 tcgtctggtg cgggggctcg gacctgacgg ccgatgaggt cctcagtcgg gccgacatcg
     2521 cgatgtatcg agccaaggag cgcggcgatg gctcgtatgc gctcttcgag caagcgatgc
     2581 acgactccgc cctcgatcgt gcgcagctgc gcagcgatct cgagggggcc gtcggacgcg
     2641 gcgagatcac gccgtggttc cagcccatct tcgatgtgac gacccgtcgc ctggtcgccg
     2701 tcgaggcgct cgcccgctgg aaccacccgg cgcgcggcct cgtggcgccg gacgacttcg
     2761 ttccgatcgc cgagctgtcg ggctcgatcg cggagatcga tcggctggtc ctccgggtcg
     2821 ctgcgggtca cgtcgccgat tggatcgaca tgatcggcgc accgctccag ctccacgtca
     2881 acatcacccc gcgcgaggcg gccgatccgg ccaccgtcga cgcgatcgcg gcggcgctcg
     2941 acgaagcgaa gctgccggcc aactgcctgg tcgtcgaggt cgtcgagacg gcgctgatcg
     3001 atgagaccgc ggtcgcgccg gtcctggccg agctgaaggc cctcggcgtc ctcctgtcga
     3061 tcgacgattt cggcagccgc tacgccgtcc tgacccagct gggccgcctg ccgatcgaca
     3121 tcgtcaagct cgatcggagc ctcgtcgcgg gcgtcgagac ggccgagggg ttccgcctcc
     3181 tcgagggaat cctgcgcctt gcccagagcc tccgacttga caccgtcgcc gagggcgtcg
     3241 agtcgaccgc cgtcatgccc atcctgcgcc gcctcggttg caccgccgcc caggggtatt
     3301 cgctgggtcg cccgatgccg gccgccgact tcgagcgacg cctggtcaag tggtcgcggg
     3361 agacgctgag cgcctgactc gtctcgaccg tcaggcgggg ccggatggga gcgtccccga
     3421 cccgcagcgg ctcccgatcg cgcgatgagt ttcgcgaagg cttgcttcga tcgccgagga
     3481 tcgacggtcc ggaatcagat cggctgaccg acgccactca tcggtggtgt cgcgggcaca
     3541 acgaatcgac gacgcgggcg agtcatcggc ggcgtaccgt ggacgcggcc cgaacctctc
     3601 gtgtcgtgga ggctccgatg tctgggcgag cagtgtcgat ctcgcctgcg gaggcgacgc
     3661 cggggcgttc acggagtcga tatgcctgag gccccggccg cgctcagatc cgccttttcc
     3721 gccctccgcc aggcgctcgc caacgacggg atccgtcggc tcgggatctc ctggacgctg
     3781 ggggtcgcgg ccgattccgc gctcctggtg gtcaccctgg tcacggtgtt caatggcggc
     3841 ggcttcgtcg ctgccgccgt tctcggcgcg gtccgcatga tccccgcggt cgtggccggc
     3901 atgctcgccg gcacgatgct cgagcgcttc cgcggtgacc ggatcctggt cgtgctcggc
     3961 ctggtccgtg cggccagcgc cggtctcacg gtctttgcga tggtcacggc ggggccgacc
     4021 ctggccgatc accacgtcac cgtggtcctc ctcttcgtgg ccgcagccgt cgctgctgcg
     4081 gcgggcgctc ccgtgcggcc gacgcaggtc accctgatgc cgacgatcgc acgcagcccg
     4141 gccgagctcg tcaccgcgaa caccgcctgg agcacgggcg aggggctcgg tgcgttcggt
     4201 gggccgttcg tcgccggcat tctcatgggg caacagctgc acatcgtcgt tgcggtcgtg
     4261 gccgccgtcg cgttcatcgt gacggccgtg gtcggcgctg gactgcggtt cgagcaggcg
     4321 gccgacgctt cgggcggggg caggcacacg acgaacggga tccggctgat ggatggcgtt
     4381 cgcgcgctcc gagcgcggcc cgtgctggcc tggtcgatgc tgggtacgtt tggccaggtc
     4441 gtgacccgtg ggctgctcag cgcg
//
LOCUS       DATAIW010000100         8163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_153676, whole genome shotgun sequence.
ACCESSION   DATAIW010000100 DATAIW010000000
VERSION     DATAIW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153676"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VHM48_03410"
     CDS             complement(<1..792)
                     /locus_tag="VHM48_03410"
                     /inference="COORDINATES: protein motif:HMM:NF026047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754483.1"
                     /translation="MTDTIARGAANQAGAHLNKNAYDEAQDFLPLKGIDHVEFWVGNA
                     RQASHYFRALWGFTPIAYAGLETGVRDRTSYVMRRNDITFVLTGALAPDGEIAEHVHR
                     HGDGVKDIAFLVDDAQSSFAETTKRGARPAIEPVELDGGEDGILRRSAVYTYGEVRHS
                     FIDRRDYHGVFAPGFRKVKKPAPAATGMSLLEIDHCVGNVDLGDMNKYVDYYRDVFGF
                     AQLIHYDDKVIHTEFSALMSKVMTNGNGRVKFPINEPASGKKKSQI"
     gene            912..1454
                     /locus_tag="VHM48_03415"
     CDS             912..1454
                     /locus_tag="VHM48_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2754484.1"
                     /translation="MQDWGMNDLAGRLRDAGSALLGLRGALVAGEPWPLSAAYGTEPE
                     ADWGPREVLAHVNEMLGYWPRQLEAVLAGDPAVAAPFGRVAADPSRIHRIEVDRERPA
                     GELLDGIDAGLADATAFVEALSPTDLARSATHPTRGEMSVGTAIEFFLADHLEGHVAQ
                     VREILARSAQREPGQTGTTG"
     gene            complement(1822..2805)
                     /locus_tag="VHM48_03420"
     CDS             complement(1822..2805)
                     /locus_tag="VHM48_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2754485.1"
                     /translation="MPATTALRSAPERAMRTSLLWLSRRRSLGRLATRLPITRSMVAR
                     FVAGETLGEALVALRKLQEAGFRTTVDVLGEAVTSVDAATAAADQYLATLDALAANGL
                     DRNASLKLSQMGLKLDPTACRENVTRVFGRAAEVEAFIRIDMEDHAYTDATLALWREL
                     RPLNAGRGDSGVVIQAALRRSPRDVGALIGEQARIRLCKGAYVEPAAVAFPAKPDVDD
                     AYATLMTELLRAGTFPALATHDERLIGRAVALAREEGIAPDRFEFQMLFGIRRDLQER
                     LLKAGFGVRVYVPFGTQWYPYFMRRLAERPANVAFLLRSVLREGRDRPSKG"
     gene            2868..3503
                     /locus_tag="VHM48_03425"
     CDS             2868..3503
                     /locus_tag="VHM48_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX2754486.1"
                     /translation="MTIPDFGSDLVGVVLRTAVVYVCLVLGFRFLGKREAGQLSTLDL
                     VVLLVISNAVQNAMVGENTSLIGGLVAAAVILVLDLALHAAADHWAPLRNALDGEPTI
                     LVEHGRILVDNLRHEGISDRELAVALRQNQLMSAEEALFVFLETNGQISVIPRRDDDD
                     ASDDGPPDLDGASKDPAVRDPAVGATHPRGGSSHGTFRRRHRRPGRLSGSG"
     gene            complement(3581..5626)
                     /locus_tag="VHM48_03430"
     CDS             complement(3581..5626)
                     /locus_tag="VHM48_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2754487.1"
                     /translation="MKQATGTGSRPSGATVAAATGGLTIERLVAVDSPREFRLHPRDR
                     VVACSQEAGGARQLVVLPLRGGSPLQVTASEKDVSDPQWSPDGRRLAYARGDEIRIVD
                     ADGSRDVLVAGHPAGVSMPRWSPDGLRIAFRSRRRGWSQVNVVDAPVPRRGRPSRDPR
                     PPEPRTVTAIGFDVEDYEWSADGATIAVATFRGPEFAHGEIHLVDVVSGDERRIAGGS
                     KEWASGPRAMPGGGFLYLSDANGWFQVVRLSPDGREKTVLTTGRRDHGEPSGGYGYAA
                     LPSPDGSRFVHAEIHDALIDLVVAPIGGATPVKRGRGRPPKNPPPVVAAGAGEIVNPW
                     PGVWRSVGFLADGAWLAAIGESQDRPQDLWLLPVPGVAQAGARARQVTNSMPAVLEAA
                     FRPDRTIAGERIGFKARDGRRIEGTLWRPAAATGKRGAVRVPTVVYPHGGPTWQAYRS
                     WVPFKQLLVREGFALLDVDFRGSTGYGREFRNANHDEWGHADAFDMVDAARWAAGQPW
                     SNGRLGIYGGSYGGYLALCALVEEPGLWQAGVDLYGDSEIAESFRHGDRPGRLDLGRM
                     MGSPDDPAKAELYRRGSPLYQAERIEAPLLILHGRKDKRVVPMMTEKMVEALQIEGKF
                     HEVHWYDDEGHGWERRENRRDAFNRIRAFLRRHLMDDQSAPDEAEAVTPSGGRSAAR"
     gene            complement(5667..6770)
                     /locus_tag="VHM48_03435"
     CDS             complement(5667..6770)
                     /locus_tag="VHM48_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2754488.1"
                     /translation="MTAPATDRLVDLRRGTLSDLRAIDFRAPSRDYWADEAAVHDRFL
                     VVWAGLDDAAWRLPGAAPSDGGGADWSILDHVGHVVDWWELATDYIADVVGGADWPSD
                     DDYYEGGDFNALNERRRARFADVAPADLRARGVVAHERARAIARRLPTKTIRSDAAWG
                     WVHQVLHGHELDHLAVLEPWADALRTRQIGNDPFGLDPQPVRSALAAGIERFWTTAGS
                     VLADVDRLIAATPDRAWTASTDGDWTFADHVAHLAGWFVEGARALETHRPGGGWADLP
                     PEGLDAFNDRQVRAARGIPPADLRAAYTDGLARLMTAIEAMIDEEWLDPEGFSWAYED
                     LHGHVRAHYAMIGPWLARLGWPSPAARAGIETA"
     gene            complement(6767..7636)
                     /gene="proC"
                     /locus_tag="VHM48_03440"
     CDS             complement(6767..7636)
                     /gene="proC"
                     /locus_tag="VHM48_03440"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX2754489.1"
                     /translation="MTALSGKTIATVGSGVMAEAMIAGLLRGALVAPDQVVASHPRPE
                     RRDHLRSEYGIRVVADNLEAIDGADVVLLGIKPQMLAKVGREIGPHLRRGQVVLSVLA
                     GATTTALIGTLGHDQVIRAMPNTPARLGKGVTVWYATPQTTAEQREQAAALLGALGTQ
                     IEVDDEKMVAMATAVSGTGPTYVFLFMEALIDAAVHLGFPRHVAHDLVVETIEGSTLF
                     AKQSQMHPAELRNMVTSPGGTSAAALHELESGRLRTVLSEAVWAAFRRTIELGDQLEA
                     SVGKPAADPPKRP"
BASE COUNT         1114 a         2916 c         2949 g         1184 t
ORIGIN      
        1 gatctggctc ttcttcttgc cggacgccgg ctcgttgatc gggaatttca cccggccgtt
       61 gccgttggtc atgaccttcg acatcagggc gctgaactcg gtgtggatga ccttgtcgtc
      121 gtagtggatc agctgggcga agccgaacac gtcgcggtag taatcgacgt acttgttcat
      181 gtcgccgagg tcgacgttgc cgacgcagtg gtcgatctcg aggaggctca tcccggtcgc
      241 ggcgggggcc ggcttcttga ccttccggaa gcccggggcg aagacgccgt ggtagtcgcg
      301 tcggtcgatg aagctgtggc ggacctcgcc gtacgtgtag accgccgacc ggcggaggat
      361 cccgtcctcg ccgccgtcga gctcgaccgg ctcgatcgcc ggccgggcgc cgcgcttcgt
      421 cgtctcggcg aacgacgact gcgcgtcgtc gacgaggaag gcgatgtcct tgaccccgtc
      481 gccgtggcgg tggacgtgct cggcgatttc gccgtcgggc gcgagggcgc cggtcaggac
      541 gaaggtgatg tcgttgcgcc gcatgacgta gctcgtccgg tcgcggacgc cggtctcgag
      601 ccccgcgtag gcgatcgggg tgaagcccca cagcgcccgg aagtagtgcg acgcctggcg
      661 ggcgttgccg acccagaact cgacgtggtc gatgcccttg aggggaagga agtcctgggc
      721 ctcgtcgtag gcgttcttgt tgaggtgagc gcccgcctgg ttggcggcgc cgcgggcgat
      781 cgtgtcggtc attcggacgg cctcgatgcg gtccggcgac ggcgacgacg ggctgggccc
      841 ggcgcgacgg ccacggaacg gcggcccggc accgggccgg gatggtctga ccgcccatgc
      901 tacgccgccc cgtgcaagac tggggtatga acgatcttgc cggccggctc cgcgacgccg
      961 gctccgcgct cctgggactc cggggtgcgc tcgtcgcggg tgagccgtgg ccgctcagcg
     1021 ccgcctacgg caccgagccc gaggccgact ggggaccgcg cgaggtcctc gcccatgtga
     1081 acgagatgct cggctactgg ccccgccagc tcgaggcggt tctcgccggc gaccctgccg
     1141 tcgccgctcc cttcggccgg gtcgcggccg atccgtcacg catccaccgg atcgaggtcg
     1201 atcgggagcg tcccgccggc gagctgctcg acgggatcga cgccggcctg gcggacgcca
     1261 cggcgttcgt cgaagccctc tcgccgaccg acctcgcccg gtccgcgacg catccgacac
     1321 gcggggagat gtccgtcggg acggccatcg agttcttcct cgccgaccat ctcgagggcc
     1381 atgtcgccca ggtccgggag atcctcgccc ggtccgcgca gcgtgaaccc ggccagaccg
     1441 ggactaccgg ataggggtcc tggtactgat tgcctattcc ctcccggccg atcgggccgt
     1501 acgttcggag tccgcgcagt gacccagtgc gcgcggcgag gaagggaccg ttcgtgttgc
     1561 agcagctcaa gtcgcagggg ttcaggatcc tcttgatcct ttcggcgctc gctagctcgg
     1621 cgctcgtgct cgaggccggt cagcgctgga agtaacccac accgtcctgt cgacggccgc
     1681 actcatcccc ggcgggcctc cgaccgcctc gcgctctgcg ctccgacgag ccgcccggtt
     1741 atcgggcggc tcttcgattc ccgggtcagc cgctggtcgc ggccaggccg cgcacgtctc
     1801 gcgcgcccgg acccgacggc ctcaaccctt cgacgggcga tcccgccctt cgcgaagcac
     1861 gctccggagg aggaacgcga cattggccgg ccgctcggcg aggcggcgca tgaagtacgg
     1921 gtaccactgg gtgccgaacg ggacgtagac gcggacgccg aagccggcct tcaggaggcg
     1981 ctcctggagg tcacgccgga tgccgaagag catctggaac tcgaaccgat ccggggcgat
     2041 gccctcctcg cgggcgagcg cgacggcgcg accgatcagg cgctcgtcgt gggtcgccag
     2101 ggccggaaac gtcccggccc ggaggagctc cgtcatcaac gtcgcgtacg catcatcgac
     2161 atcgggcttg gccggaaagg cgaccgcggc cggctcgaca taggcgccct tgcagagccg
     2221 gatccgggcc tgctcgccga tcagagcgcc gacatcccgc gggctgcgcc gcagcgcggc
     2281 ctggatcacg acgcccgagt cgccgcgacc ggcgttgagc ggccgcagct cgcgccacag
     2341 ggcgagcgtc gcgtcggtgt aagcgtggtc ctccatgtcg atccggatga acgcctccac
     2401 ctcggcggcg cggccgaaca ctcgggtgac gttctcacga caggccgtcg ggtccagctt
     2461 gaggcccatc tggctgagct tgagactggc gttgcggtcg agcccgttcg cggcgagcgc
     2521 gtcgagcgtc gcgaggtact gatcggcggc ggccgtcgcc gcgtcgaccg aggtgaccgc
     2581 ctcgcccagc acgtcgacgg tcgtccggaa gccggcctcc tggagcttgc ggagggccac
     2641 cagggcctct ccgagcgtct cgccggcgac gaaccgggcg accatcgagc gggtgatcgg
     2701 cagccgggtc gccagccggc cgagggaccg acggcgggac agccacagca acgacgtgcg
     2761 catcgcccgc tcgggcgcgg agcggagagc ggtggttgcc ggcacggatc gatggtagcc
     2821 gttgggcggg cccgctgacg cctcgaccag tcggctagga tcgaccggtg acgatccccg
     2881 acttcggctc ggacctggtg ggcgtcgtcc tgcgcacggc cgtggtctac gtctgcctgg
     2941 tgctcggctt ccggttcctc ggcaagcgcg aggccgggca gctctcgacc ctcgatctgg
     3001 tcgtgctcct ggtcatctcc aacgcggtcc agaacgcgat ggtcggcgag aacacgtcgc
     3061 tcatcggcgg cctcgtcgct gccgccgtca tcctcgtcct cgacctcgcc ctccatgccg
     3121 cggccgatca ctgggcgcca ctccgcaacg ccctcgacgg cgagccgacg atcctcgtcg
     3181 agcacggccg gatcctggtg gacaacctgc gccacgaggg gatcagcgac cgcgagctgg
     3241 ccgtcgccct ccgccagaac cagctgatga gcgccgagga ggcgctcttc gtcttcctcg
     3301 agacgaatgg ccagatcagc gtcatcccgc gacgtgatga tgacgacgcg agcgacgacg
     3361 gcccgcccga cctcgacggc gcgagcaagg atcccgccgt ccgcgacccg gccgttgggg
     3421 ctacgcaccc tcgtggcggc tcttcacacg ggaccttccg ccggcggcac cgccggcccg
     3481 gccggctgtc cggatccggc tgatcttcgt cccggaacgg gcgtctattc cggacgttcg
     3541 gcgcgtggag cgggtcgcgg gcaccggacc cggatccgcc tcagcgcgcg gcgctccggc
     3601 caccagaggg cgtgaccgcc tcagcctcgt ccggcgcgga ttggtcatcc atcaggtggc
     3661 gacgcaggaa ggctcggatc cggttgaagg cgtcgcgccg gttctcgcgc cgctcccagc
     3721 cgtggccctc gtcgtcgtac cagtggacct cgtggaactt gccctcgatc tggagcgcct
     3781 cgaccatctt ctcggtcatc attggcacga cccgcttgtc cttgcggcca tggaggatca
     3841 ggagcggcgc ctcgatccgc tcggcctggt agagcggtga gccacgccgg tagagctcgg
     3901 ccttggccgg atcgtccggc gagcccatca tccggccgag atcgagccgg ccgggtcgat
     3961 cgccgtggcg gaagctctcg gcgatctccg agtcgccgta gaggtcgacg ccggcctgcc
     4021 agaggccggg ctcctcgacg agcgcgcaca gggccagata gccaccgtac gatccgccgt
     4081 agatcccgag ccggccgttc gaccacggct ggcccgccgc ccaacgagcg gcgtcgacca
     4141 tgtcgaaggc atcggcgtga ccccattcgt catggttggc attgcggaac tcccggccgt
     4201 agccggtcga gccgcgaaaa tccacgtcga gcaaggcgaa gccctcacgg acgaggagct
     4261 gcttgaacgg aacccaggat cgataggcct gccaggtcgg accgccgtgc gggtagacga
     4321 ccgtcgggac ccggaccgcg ccgcgcttgc cggtcgcggc ggccggtcgc cacagcgttc
     4381 cctcgatccg ccggccgtcg cgggccttga agccgatccg ctcgccggcg atcgtgcggt
     4441 ccggccggaa ggccgcctcg aggaccgccg gcatggagtt ggtgacctgg cgggcacgtg
     4501 cgccggcctg cgccaccccg ggcaccggca ggagccacag gtcctgcggc cgatcctggc
     4561 tctcgccgat cgcggcgagc catgccccat cggccaggaa cccgaccgag cgccagacgc
     4621 ccggccacgg gttgacgatc tcgccggcac cggcggcgac gaccggcggc gggttcttcg
     4681 gcggccggcc gcggccgcgc ttgaccggcg tcgccccgcc gatcggggcc acgacgagat
     4741 cgatcagcgc gtcgtggatc tcggcgtgaa cgaaccggct gccgtcgggc gacgggaggg
     4801 ccgcgtagcc gtacccgccg ctcggctcgc cgtgatcgcg gcgaccggtc gtcaggacgg
     4861 tcttctcgcg tccgtcgggg gacagccgga cgacctggaa ccagccgttg gcgtccgaca
     4921 ggtagaggaa cccgccgccg ggcatcgccc gcgggccgct cgcccattcc ttgctgccgc
     4981 cggcgatccg ccgctcgtct ccgctgacga cgtcgacgag gtggatctcg ccgtgcgcga
     5041 actcggggcc acggaacgtg gccaccgcga tcgtcgcccc gtcggccgac cactcgtagt
     5101 cttcgacatc gaagccgatc gcggttaccg tccgtggctc cggtgggcgc gggtcgcgcg
     5161 acgggcgacc ccgccgcggg accggcgcgt caacgacgtt gacctgcgac cacccgcggc
     5221 ggcgcgagcg gaaggcgatc cgcaatccgt cgggcgacca gcgcggcatt gacactccgg
     5281 ccgggtggcc tgcgacgagc acgtcgcggc tgccgtcggc atcgacgatc cggatctcgt
     5341 cgccgcgggc gtaggcgagg cggcgaccgt cgggcgacca ctgcggatcg ctgacgtcct
     5401 tctccgacgc ggtgacctgg agcggactgc cgccccggag cggcagcacg acgagctgcc
     5461 gggcgccgcc ggcctcctgg ctgcaggcga cgacccggtc gcgcgggtgg agccggaact
     5521 cccgcgggct gtcgaccgcg acgagccgtt cgatcgtcaa cccaccggtc gcggcggcga
     5581 ccgtcgcccc ggatggacgg gagcccgtcc cggttgcctg cttcatcgat cctcctccgg
     5641 gcgcaccccg tggcacgccg tcgacgtcac gcggtttcga ttccggctcg tgcggcgggc
     5701 gacggccagc cgagacgagc cagccacggg ccgatcatcg cgtagtgggc gcggacgtgc
     5761 ccgtggagat cctcgtaggc ccagctgaac ccctccgggt cgagccattc ttcgtcgatc
     5821 atcgcctcga tcgccgtcat gaggcgggcg aggccgtccg tatacgccgc ccgcaggtcc
     5881 gcgggtggga tgccgcgggc ggcgcggacc tggcgatcgt tgaacgcatc gagcccttcg
     5941 ggcggcagat ccgcccaccc gccgccgggg cgatgcgtct cgagcgcccg cgcgccctcg
     6001 acgaaccagc ccgcgaggtg ggcgacgtga tcggcgaacg tccagtcgcc gtcggtggag
     6061 gcggtccacg cccggtccgg cgtggcggcg atcaaccgat ccacatcggc gagcaccgag
     6121 ccggcggtcg tccagaaacg ctcgatcccg gcggcgaggg ccgaccggac gggctgcggg
     6181 tccagcccga acgggtcgtt cccgatctgg cgggtgcgca gggcgtccgc ccacggctcg
     6241 aggacggcca ggtggtcgag ctcgtggccg tggaggacct ggtggaccca accccacgcc
     6301 gcgtcgctcc ggatggtctt ggtcggaagc cgtcgggcga tcgcgcgggc gcgctcgtgg
     6361 gcgaccacgc cccgcgcccg cagatcggcc ggcgcgacgt ccgcgaagcg ggcacgccgg
     6421 cgctcgttga gcgcgttgaa gtcgccgccc tcgtagtagt cgtcgtcgga cggccagtcc
     6481 gctccgccga cgacgtcggc gatgtagtcg gtcgcgagct cccaccagtc gacgacgtgg
     6541 ccgacatgat cgaggatcga ccagtcggcg cctccgccgt cggatggggc agccccgggc
     6601 aaccgccacg ccgcatcgtc gaggccggcc cagacaacga ggaagcggtc gtggaccgcg
     6661 gcttcatccg cccagtagtc gcggctcggg gcgcgaaagt cgatcgcgcg gaggtcgctc
     6721 agggtgcccc gccggaggtc gacgaggcga tccgtggcgg gcgccgtcac gggcgcttgg
     6781 gcggatcggc ggctggcttc ccgacgctgg cctcgagctg gtcgcccagc tcgatcgtcc
     6841 gccggaaggc ggcccagacg gcctcggaca ggacggtccg cagccgcccg gactcgagct
     6901 cgtggagcgc cgccgccgag gtgccgcccg gcgaggtgac catgttccgc agctcggccg
     6961 gatgcatctg tgactgcttg gcgaacaggg tgctgccctc gatcgtctcg acgacgaggt
     7021 cgtgggcgac gtggcgtggg aagccgaggt ggacggcggc gtcgatcagg gcctccatga
     7081 acaggaagac gtacgtcggg ccggtgccgg agaccgccgt ggccatcgcc accatcttct
     7141 cgtcgtcgac ctcgatctgg gtgccgaggg cgccgaggag ggcggccgcc tgctcgcgct
     7201 gctcggccgt cgtctggggc gtcgcgtacc agacggtgac gcccttgccg agccgagccg
     7261 gcgtgttcgg catcgcccgg atcacctggt cgtggccgag agtgccgatc agggccgtgg
     7321 tcgtggcgcc cgccaggacg gacaggacga cctggccgcg gcggagatgc ggcccgatct
     7381 cgcgcccgac cttcgcgagc atctggggct tgatgccgag gagcaccacg tcggcgccgt
     7441 cgatcgcctc gaggttgtca gcgaccaccc ggatcccgta ctccgagcga aggtgatcgc
     7501 gccgctccgg gcgcggatga ctggccacga cctggtcggg cgcgacgagc gctccccgga
     7561 gcaggccggc gatcatcgcc tcggccatga ccccggagcc gaccgtcgcg atcgtcttgc
     7621 cgctgagtgc cgtcatcggg ccgagtatcg cacgcgattc cgggtcggag cggcggagcg
     7681 ggccgtcccg tgggacgcag atgccagtcc agcgcggtcc tggcggtgca atctgccggc
     7741 gtcgacatcg atgtcatcga tacttgggcg gttgttcggg ccgcaggacg ggaattcgag
     7801 cctgccgtcg gcccgatcga gcgccagtcc gtgcggggcc atggctcgat ccgttcgtcg
     7861 gtcttccgat tcgggctcct gagcatcccg cagccagcca gatatctatc cggtcgatac
     7921 cggacccgcc aacgctcggg cgacgcagat cccgccgcga ctggcaccgg gcatcgtcca
     7981 gcgcaaggat ccgggagccg tggcggctcc gatcgcccgg cgccggaacc gcggatcgac
     8041 acggcggtgc tccgctggat cgggcgcccg gcgcctgaac cgggatccgg gcgatcaggc
     8101 gcggcgaccc gtggcgagca cgaagaaacg gccggtcgtc gtgacctcga cgtcgcggaa
     8161 ccc
//
LOCUS       DATAIW010000101         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_94064, whole genome shotgun sequence.
ACCESSION   DATAIW010000101 DATAIW010000000
VERSION     DATAIW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94064"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHM48_03445"
     CDS             <1..423
                     /locus_tag="VHM48_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BtuF-related (seleno)protein"
                     /protein_id="HEX2754490.1"
                     /translation="SATASQVVGLEWLDPPFAVGHWVPDQIRRAGGWDVLGHDGSPAR
                     PTTWDAVAEVDPDLLLLMPCGYHLRETVDEWARVSLPEWIGELSAIQRGHLIALDGSS
                     YFSRPGPRVVDGIEMLAEIFDPEAFRDISPPTGWTPVA"
     gene            438..1349
                     /locus_tag="VHM48_03450"
     CDS             438..1349
                     /locus_tag="VHM48_03450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2754491.1"
                     /translation="MARAPHPTFDCLWCGRSWTVRAPDDLEGFARLCPDCLGKAGDNE
                     FLRFRLRAALEARAASSSRAPSVTPRAVVPEMTAASGAMVAYYESRASEYDDWYLRRG
                     RYEHGPIHDAAWNAELDAAGRWLDSLPVGSRIVELAAGTGWWSPLLASKGELWMYDAA
                     EAPLERARARLVAHRLRAHLHVRDAWAEPDGAPADALFAGFWLSHVERDRTGAFLELA
                     ARWLAPGGRLALIDSLPDPRSGAVDHPTPADDRSLRRLDDGREFTIVKVYRSADEIAA
                     GLAAAGFRDVEVTTTGRFFVLATGRRG"
BASE COUNT          199 a          630 c          585 g          248 t
ORIGIN      
        1 tcggcgaccg cgtcccaggt cgtcggcctg gagtggctcg atccgccgtt cgcggtcggc
       61 cactgggtcc ccgaccagat ccggcgagcc ggcggctggg atgtcctcgg ccacgatggc
      121 agcccggccc ggccgacgac ctgggacgcg gtcgccgagg tcgaccccga cctgctcctc
      181 ctgatgccgt gcggctacca cctgcgcgag acggtcgacg agtgggcccg cgtctccctg
      241 cccgagtgga tcggcgagct gtcggcgatc cagcgtggcc acctgatcgc gctcgacggc
      301 tcgtcgtact tctcgcgacc cggcccgcgg gtcgtcgacg ggatcgagat gctggccgag
      361 atcttcgacc cggaagcctt ccgcgacatc tccccgccga ccggctggac gccggtcgcc
      421 tgatcgcggc ttcgtcgatg gcgcgcgcgc cgcatccgac gttcgactgc ctgtggtgcg
      481 gccgatcgtg gacggtccgc gcccccgatg acctcgaagg attcgccagg ctctgcccgg
      541 actgcctggg caaggccggc gacaacgagt tcctccggtt ccggttgcgg gccgcgctcg
      601 aggcccgcgc ggcgtcgtcg tcgcgcgcgc cgtcggtgac accgcgggcc gtggttcccg
      661 agatgaccgc cgcgagcggc gccatggtcg cctattacga atcccgggcg tctgaatacg
      721 acgactggta tctccgacgc ggccggtacg agcacggccc gatccacgac gccgcctgga
      781 acgccgagct ggacgcggcc ggtcgctggc tcgacagcct gccggtcggg agccggatcg
      841 tcgagctcgc cgcaggcacc gggtggtggt cgccgctcct ggcgtcgaag ggtgagctct
      901 ggatgtacga cgccgccgag gcgcccctcg agcgcgcccg cgcgcgcctc gtcgcccatc
      961 gcctccgtgc ccacctccac gtccgcgacg cctgggccga gccggacggc gcgcccgccg
     1021 acgccctgtt cgccgggttc tggctcagcc atgtcgagcg cgatcgaacg ggggcgtttc
     1081 tcgagctcgc ggcccgttgg ctggcgccgg gcggccggct cgccctgatc gattcgctgc
     1141 ccgacccgcg ctccggcgcc gtcgaccacc cgacgcccgc cgacgatcga tccctgcgcc
     1201 gcctcgacga cggccgcgag ttcacgatcg tcaaggtcta ccgatccgcc gacgagatcg
     1261 ccgccgggct ggccgcggcg gggttccgcg acgtcgaggt cacgacgacc ggccgtttct
     1321 tcgtcctcgc cacgggtcgc cgcggctgag cggggccgcg gcgggcaccg gccggacacg
     1381 gcccagcgcc cggtcacctc gagaactcga catcgatgcg attgatatct cgcggtttgc
     1441 cgacccgatt gggcgcggaa gagttcctcg cctgccgatt ccggacccga ctcagccgct
     1501 gttcacgctc gcccgaacgc gacggatggc gacggcccgg cgatgtcggc gcctgggggc
     1561 ctgccacggg ctggcatacc gatcacgtcg ctgcggcccg acgcaggatg tccgaccgag
     1621 ttgttccgcg ggacggggct tccggccggt cgcaccgcga at
//
LOCUS       DATAIW010000102         7339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_34550, whole genome shotgun sequence.
ACCESSION   DATAIW010000102 DATAIW010000000
VERSION     DATAIW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34550"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VHM48_03455"
     CDS             <1..247
                     /locus_tag="VHM48_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2754492.1"
                     /translation="QAIKWANDVRFGLAASVWTSDAGRGMRVAKAIQFGTVWINDHFM
                     LVSEMPHGGFKESGYGKDQSMYAVEDYTVVKHVMLKI"
     gene            complement(614..1558)
                     /locus_tag="VHM48_03460"
     CDS             complement(614..1558)
                     /locus_tag="VHM48_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2754493.1"
                     /translation="MPHPVRIGAAFWVQRTDWPSLRVAVETADAAGADDLWIDDHLLS
                     DEGHSSDGKLEGWTTLAATAAVTARARLGLLVAANTFRNPGLTAKLATTLDQISGGRA
                     ILGLGGGWFEEEHRAFGLDFGSGFGERLDRLAESVEIVRRLLDGERFSHEGRFYQLRD
                     ALAAPRPVQPHLPILIGGSGPQKTLRIVARHADLWNAYGTPDSLALTDAILRERCAEV
                     GRDQATIERTVNLNVVVRSTREEAERVWAGYAAAHQPRGGEGRLVVGGPVEDVAAALA
                     EYAAVGFRHPILIFRTPWDLETIRSIGAIRETLARALD"
     gene            1582..2544
                     /locus_tag="VHM48_03465"
     CDS             1582..2544
                     /locus_tag="VHM48_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX2754494.1"
                     /translation="MARSRLAAPPTLAAPALAAPGVPALPPDARAAVLAWFDERGRAL
                     AFRGTRDPYAILVSEVMAQQTQISRVLEAWSRFLETFPTVETLAAATPADVLRAWRGM
                     GYDRRALNLRRAARLIVDEHGGQVPSDLEALKRLPGVGPYTARAVASIAFGAPVGAVD
                     TNVRRVLGRALAGHDAAALPPARLQSIADASVDPDRPGDWTHAVMDVGATICRPRSPL
                     CSECPLRRWCRFATEVVDDPAVALRPARARAPAIPFAATTRWLRGRIVDRLRGAGGAD
                     WSIVTSPIGDHDAIAVRTALGALARDGIVELDPTDPDRARLATI"
     gene            2743..3507
                     /locus_tag="VHM48_03470"
     CDS             2743..3507
                     /locus_tag="VHM48_03470"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX2754495.1"
                     /translation="MPADDPDLLGLDLRELRRHWARTAARAPMTAAAMTGADRRAQAL
                     GIPGSQLMEHAGVAVAAAARALAIDTGRWDHGPIVILVGPGNNGGDGLVAARHLAAAG
                     ARVVIALVASGARPTTPDAARNWDRVVRDERVTIVHTPVARDVAMLGHGIEKAAIIVD
                     ALLGTGVRGPLREPIRSAVELINRARGEMVPILAVDTPTAIDLTSGDPSDPAVRADLT
                     ITFHRPKTGLATRNGKALAGRVLVAPIGIPQEADRG"
     gene            3500..3766
                     /locus_tag="VHM48_03475"
     CDS             3500..3766
                     /locus_tag="VHM48_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754496.1"
                     /translation="MAEAPESGGRLPGLRDEGPRAPGLREALLVGELVVVVVLLAGVA
                     TYLLPGDTRDLVLRTPLLIMVLIGGTAGVLWRISRPGRPGGPDR"
     gene            3821..5446
                     /locus_tag="VHM48_03480"
     CDS             3821..5446
                     /locus_tag="VHM48_03480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2754497.1"
                     /translation="MTAAPVEARRRDLAALVDRPLDVLVVGGGIVGCGAALDAATRGL
                     RVGLVERDDIAVGTSSRSSRLIHGGLRYLEQYRFGLVREALTERSRILHLAPHLVTIE
                     PLLFPLFGTPVVTRAFYQAGMTLYDMLGARKDGGWHRHLSVPEVVGHAPAMRRKGLRG
                     GLMFHDGVEDDARYTLAVARSAQEDGATIVTRATAESLVEEGGRVVGARISDRLTDEQ
                     LEIRARVVIDATGVWGADPESPLSGGSTRLLPSRGAHLVVPRSRIPSDVGLTIRVPGK
                     VVFLVPWPHFWLVGTTDAPYSGPIDRPTAGQHEVDELLAAVNHVLDVDLGRRDVVGTF
                     AGLRPLIAPSGGSTITASREHRVVVEGNGLVRVSGGKYTTYRLMAQQTIDAALGVLGA
                     RAAERPTRTTTRRLVGAADRPELDRIAAGLAAAASLEPAIAARLVARHGTQAPEVVAL
                     GREAGLLGRLVDGEDHLEAEVAWAARHELALSIDDVLARRMRIVHELPDRAAAIAPRV
                     AAILGAELGWDAARQASEIESFLAGARREFEIP"
     gene            5443..6957
                     /gene="glpK"
                     /locus_tag="VHM48_03485"
     CDS             5443..6957
                     /gene="glpK"
                     /locus_tag="VHM48_03485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2754498.1"
                     /translation="MSDHGAGAPVVLAFDEGTTSARTIAVDRAGNVVAVAQREFEQRY
                     PAPGDVRHDPETIWDAQLATAREVIAAVGGAEHVIAIGITNQRETAVVWDRSSGRAVA
                     DAIVWQSRVTAPFCEALRAAGHEELVRARTGLPIDAYFSGPKFRQILVEDPALRPRAQ
                     RGELAAGTVESFLIWRLTGGRTHVTDVSNAARTLLLDIHRGAWDEDLVALMEVPPALL
                     PEVRSCAEVYAQTDPSIFGRAIPIAAAAGDQQAATFGQACFAPGEAKVTLGTGAFLLA
                     NTGEIAVASRHGLLTTVAWQLGPDAPRVYALEGSVFVTGAAVQWLRDGLGLLRETAGI
                     EALAGSVADSGGVVIVPAFVGLGAPYWDPDARGAIFGLTRGSGAAHLARATLDAIAQQ
                     VRDVVEAMDADLGSPLLGLRVDGGGAGDLVCGLIADQLGRPVERPVVRETTAFGVAAM
                     AGLAVGFWNSPAEIGAVRRVERRFEPSNDETARAASRRDWRRAVERSRGWAAAD"
BASE COUNT          947 a         2679 c         2669 g         1044 t
ORIGIN      
        1 gcaggcgatc aagtgggcga acgatgtccg cttcggcctt gcggcctcgg tctggacgtc
       61 cgacgccggc cgcgggatgc gagtggccaa ggcgatccag ttcgggacgg tctggatcaa
      121 cgaccacttc atgctcgtgt cggagatgcc ccacggtggc ttcaaggagt cgggctacgg
      181 caaggaccag agcatgtacg cggtcgagga ctacacggtc gtcaagcacg tgatgctcaa
      241 gatctgaggt cgcgtgctgg gtcgcccgcg gaggccggcg ccgcgcgggc ggcgcccaat
      301 gcgcattcgg tgagcactgc gacgaaggag gcgtcgcccg gacggcagat cggcctccga
      361 gtggcagccc gttagccttc cgcggctgat tctcgccggg ccaaccggac gatccgggtc
      421 tccttcgccg caacgcgcgc tgtgtgcgca ggcggcggcg ggtcggccag ggattcccac
      481 tcgattctcg ccccgcgtcc agccgacccg aattggccca tgttcgtcgc cgttctcgtc
      541 cggcgggcga aaccggcaga acgacgccga accatgctcc gtacgcccgc ccggcgggag
      601 gccggggccg tgctcagtcg agggcgcgtg ccagcgtctc ccggatcgcg ccgatcgagc
      661 ggatcgtctc gagatcccac ggcgtgcgga agatgaggat cgggtggcgg aagccgaccg
      721 cggcgtactc ggcgagggcg gcggcgacgt cctcgaccgg tccaccaacg acgaggcgtc
      781 cctcgccgcc ccgcggctgg tgggcggcgg catatccggc ccagacacgc tcggcctcct
      841 cgcgggtcga gcggacgacg acgttgaggt tgacggtccg ctcgatcgtc gcctggtcgc
      901 gcccgacctc ggcgcagcgc tcccgcagga tcgcgtcggt cagcgcgagg ctgtcaggtg
      961 tgccataggc gttccacagg tcggcatgac gggcaacgat ccggagcgtc ttctgtggcc
     1021 cggagccgcc gatgaggatc ggcaggtgcg gctggaccgg acgcggcgcc gcgagggcgt
     1081 cgcggagctg gtagaaccgg ccctcatggc tgaagcgctc gccgtcgagc aggcgccgga
     1141 cgatctcgac cgactccgcg aggcggtcca gccgctcgcc gaacccgctc ccgaagtcga
     1201 gcccgaacgc ccggtgctcc tcctcgaacc agccaccgcc gaggccgagg atcgcgcggc
     1261 cgccactgat ctggtcgagg gtcgtggcga gcttggcggt cagccccggg ttgcggaacg
     1321 tgttcgcggc gacgagcaat ccgagcctgg cacgggccgt gaccgcggcc gtcgccgcca
     1381 gcgtcgtcca gccctcgagc ttgccgtcgg aggagtgacc ctcgtcggag aggaggtggt
     1441 cgtcgatcca gaggtcgtcc gctcccgcgg catcggccgt ctcgaccgcg acgcggagtg
     1501 acggccaatc ggtccgctgg acccagaacg cggcgccgat ccggacgggg tggggcatcg
     1561 gccgatggta cgctcgagac gatggcccga tcgcgactcg cggcacctcc gacgctcgcc
     1621 gcgccggcac tcgccgctcc gggtgtgccg gccctcccgc ccgacgcgcg cgccgccgtc
     1681 ctggcgtggt tcgacgagcg tggccgcgcg ctcgcattcc gtggaacccg cgatccctac
     1741 gcgatcctcg tgtccgaggt catggcccag cagacgcaga tctcccgcgt gctcgaggcc
     1801 tggtcccggt tcctcgagac gttcccgacg gtcgagacgc tcgccgcggc caccccggcc
     1861 gacgtcctcc gggcgtggcg ggggatgggc tacgaccgcc gggcgctcaa cctgcgacgc
     1921 gcggcgcggc tgatcgtcga cgaacacggc gggcaggtcc cgagcgacct cgaggcgctc
     1981 aagcgattgc ccggtgttgg gccgtacaca gcgcgggcgg tcgcgtcgat cgcgttcggc
     2041 gctcccgtcg gcgccgtcga cacgaatgtc cggcgggtgc tcggtcgggc cctggccggt
     2101 catgacgccg cggcgcttcc gccggcccgg ctgcaatcga tcgccgacgc gtccgtcgat
     2161 cccgatcgac ccggcgactg gacccacgcg gtgatggacg tcggggcgac gatctgccgg
     2221 ccgcgatcgc cgctttgcag cgaatgcccg ctgcggcggt ggtgccggtt cgcgacggag
     2281 gtggtcgacg acccggccgt cgccctccgg ccggctcgcg cccgcgcgcc cgcgatcccc
     2341 ttcgcggcca ccacccgctg gctgcgcggc cggatcgtcg atcgcctccg gggtgccggc
     2401 ggggccgatt ggtcgatcgt gacctctccg atcggcgatc acgacgcgat cgccgtccgg
     2461 acagctctcg gcgccctggc ccgcgacggg atcgtcgagc tcgacccgac cgatcccgac
     2521 cgggcacgac tcgccacgat ctgaccaggc gggagcgtcg ggccggatct catgcaaccg
     2581 ggcgacgatc tcgggcctgc gagccggtgc tgtcctcgat cctgtggcgg ccgagtcggc
     2641 gtccgcggcg gtcggccggc ccggatcgac tacgcttcgg gcgtcatgac aacgcctgcc
     2701 ccgtcctcgc gcgcgcccgc gggctcatct tcgaccgatc cgttgccagc cgatgacccg
     2761 gacctcctcg gtctcgacct gcgcgagctg cgccgccatt gggcgcgaac cgccgcccgg
     2821 gcgccgatga ccgcggccgc gatgacaggc gcggaccgcc gggcccaggc gctcgggatc
     2881 ccgggttcgc agctcatgga gcacgccgga gtcgccgtgg ccgcggccgc gcgggccctc
     2941 gccatcgaca ccgggcgatg ggaccacggc ccgatcgtca tcctcgtcgg gccgggcaac
     3001 aacggcggcg atgggctcgt cgccgctcgt cacctggccg ccgcaggcgc ccgggtcgtg
     3061 atcgcgctcg tcgcatcggg cgcccgcccg acgaccccgg atgcggcccg caactgggac
     3121 cgcgtcgtcc gcgacgagcg ggtcacgatc gtccataccc ccgtcgcccg cgacgtcgcg
     3181 atgctcggcc acgggatcga gaaggcggcc atcatcgtcg acgcgctgct cgggaccggg
     3241 gtccgcgggc cgctccgcga gcccatccgg tcggccgtcg agctgatcaa ccgggcgcgt
     3301 ggcgagatgg tcccgatcct tgccgtcgac acgccgaccg cgatcgacct gaccagcggc
     3361 gacccttccg acccggccgt ccgggcggac ctgacgatca cgttccatcg ccccaagacc
     3421 ggcctggcga cccgcaacgg caaggcgctc gccgggcgcg tgctcgtggc gcccatcggg
     3481 atcccgcagg aggccgaccg tggctgaggc tcctgagtcg ggcggccggt tgccgggcct
     3541 gcgcgacgag ggtcctcggg cgcccggcct gcgtgaggcg ctcctcgtcg gcgagctcgt
     3601 cgtcgtggtc gtcctgctcg ccggcgtcgc gacgtacctg ctccccggcg acacccgcga
     3661 cctcgtcctg cggacgcccc tcctgatcat ggtcctgatc ggcggaacgg caggggtcct
     3721 gtggcggatc agccggcccg gccggcccgg cgggcctgac cgatgaggag ccggccgcga
     3781 ccgtgggcgc gatgaggatc cggcccggac gccgtcgtcg atgaccgcgg cacccgtcga
     3841 ggcccggcgt cgtgacctcg cggccctcgt cgatcgaccg ctcgacgtcc tcgtcgtcgg
     3901 cggcggcatc gtcggctgtg gcgccgcgct ggatgccgcg actcggggtc tgcgggtcgg
     3961 gctggtcgag cgcgacgaca tcgccgtcgg gacgtcgtcc cgctcgtcgc gcctgatcca
     4021 cggcggcctg cgctacctcg agcagtaccg gttcggcctc gttcgggaag ccctgaccga
     4081 gcggtcgcgg attctccacc tcgcgcccca cctcgtgacg atcgagccgc tgctgttccc
     4141 gctgttcggg accccggtcg tgacccgcgc cttctaccag gccggaatga ccctctacga
     4201 catgctcggc gcccgcaagg acggtggctg gcatcgccac ctctccgtgc cggaggtcgt
     4261 cggccatgcg ccggcgatgc ggcgcaaggg tctccgcggc ggcctgatgt ttcacgacgg
     4321 ggtcgaggac gacgcgcgat acacgctcgc cgtcgcccgc tcggcccagg aggacggcgc
     4381 gaccatcgtg acccgggcga ccgccgaatc gctggtcgag gagggcggac gggtcgtcgg
     4441 ggcgcggatc agcgatcggc tcacggacga gcagctcgag atccgggcac gggtcgtcat
     4501 cgacgcgacc ggggtctggg gcgccgatcc ggaatcgccg ctgtcgggcg gctcgacccg
     4561 cctgctgccg agccgcggag cccacctggt cgtgccccgc tcgcggatcc cgagcgatgt
     4621 cggcctgacg atccgggtcc ccggcaaggt cgtgttcctc gtgccgtggc cgcatttctg
     4681 gctcgtcggc acgaccgacg cgccgtattc cggcccgatc gaccggccga ccgccggcca
     4741 gcacgaggtc gacgagctgc tcgcggcggt caaccatgtc ctcgacgtcg acctcggccg
     4801 gcgcgatgtc gtcgggacct tcgccggtct ccggccgctg atcgcgccct cgggcggctc
     4861 gacgatcacg gcgtcgcgcg agcaccgcgt cgtcgtcgag ggcaacgggc tggtccgggt
     4921 cagcggcggc aagtacacga cctatcggtt gatggcccag cagacgatcg atgcggcact
     4981 cggcgtcctc ggcgcgcgtg ccgccgagcg cccgacgcgg acgacgacgc gccgtctcgt
     5041 gggcgcggcc gatcggccgg agctcgatcg gatcgccgcg ggcctggccg ccgcagcgtc
     5101 actggagccg gcgattgccg cccgactcgt cgcccgccac gggacgcagg cgcccgaagt
     5161 cgtcgcactc ggccgcgagg cgggcctgct cgggcggctg gtcgatggcg aggaccatct
     5221 cgaagcggag gtggcctggg ccgctcggca cgagctcgcg ctctcgatcg acgacgtcct
     5281 tgcccgccgg atgcggatcg tccatgagct cccggaccgt gcagccgcga tcgcgccgcg
     5341 ggtcgcggcc atcctcggcg ccgagctcgg ctgggacgcc gcccgccagg cgagcgagat
     5401 cgagtcgttc ctcgccggtg cccgccgcga gttcgagatc ccttgagcga tcacggcgcg
     5461 ggcgcaccgg tcgttctcgc gttcgacgaa gggaccacga gcgcccggac gatcgcggtc
     5521 gaccgggccg gcaacgtcgt cgccgtcgcc cagcgcgagt tcgagcagcg ttacccggcc
     5581 ccgggcgacg tgcgccacga ccccgagacg atctgggatg cgcaattggc aacggctcgc
     5641 gaggtgatcg cggcggtcgg cggcgccgag cacgtcatcg ccatcggcat cacgaaccag
     5701 cgcgagaccg cggtcgtctg ggaccgctcg agcgggcgcg cggtggcgga cgcgatcgtc
     5761 tggcagagcc gggtgacggc gccattctgc gaggcgctcc gcgcggcggg tcacgaggag
     5821 ctcgtcaggg cgaggaccgg tctcccgatc gacgcctact tcagtggccc caagttccgc
     5881 cagatcctgg tcgaggatcc ggccctccgg ccgcgagctc agcggggaga gctggcagcg
     5941 ggcacggttg aatcgttcct gatctggcgg ctgacgggtg gccggaccca cgtgaccgac
     6001 gtctcgaacg cagcgcgaac gctcctcctc gacatccatc gaggcgcctg ggacgaggac
     6061 ctcgtcgcgc tcatggaggt cccgccggcg ctcctgcccg aggtccgcag ctgcgccgag
     6121 gtctacgctc agaccgaccc gtcgatcttc ggccgcgcca tcccgatcgc ggctgcggcg
     6181 ggtgaccagc aggcggcgac attcgggcag gcgtgcttcg cgcccggtga ggccaaggtc
     6241 accctcggga ccggcgcctt cctgctcgcc aacacgggcg agatcgcggt cgcctcgcgc
     6301 cacggactcc tcaccacggt cgcctggcag ctcgggccgg acgcaccgcg cgtgtacgcc
     6361 ctcgagggct cggtgttcgt gaccggcgcc gcggtccagt ggctgcgcga tggactcggg
     6421 ctgttgcgcg aaacggccgg gatcgaggcg cttgccggct cggtcgccga cagcggcggc
     6481 gtcgtgatcg tgccggcctt cgtcgggctc ggggcgccgt actgggaccc cgacgcgcgg
     6541 ggagcgatct tcggcctgac ccgcggcagt ggcgccgccc acctcgcgcg ggcgacgctc
     6601 gacgcgatcg cccagcaggt ccgcgacgtc gtcgaggcga tggacgctga cctcggctcg
     6661 ccgctgctgg gcctgcgggt cgacggtggc ggcgccggcg acctcgtctg cggcctgatc
     6721 gccgaccagc tcggacgtcc ggtcgagcgg ccggtcgtcc gcgagacgac ggcattcggg
     6781 gtggccgcca tggccgggct ggcggtcggc ttctggaaca gcccggccga gatcggcgcc
     6841 gttcgccggg tcgagcgtcg gttcgagcca tcgaacgacg agaccgcgcg ggccgccagt
     6901 cgccgcgact ggcggcgtgc cgtcgagcga tcgcgcggct gggctgccgc cgactgacgc
     6961 cttcggcggc gtcccggcag gccgccgatt ggcggtttct gcctcgatcg gcgaaaatac
     7021 ccctgtacaa gtccgtcgtc agtcgtctag actcccgcgc ggaaaggccg tgaaagcgtt
     7081 cacggcaaga aaagacgtag ctcgacgacg actcggactc ctctcacggc tgacgcgaga
     7141 aaacccggag ggatcgcacg gctaccgaga ggagttttcg tttcgtgtac aacgataaga
     7201 atctgacctg cgccgattgc ggccaggagt tcgtgttcac cgcgagtgag caggacttct
     7261 acgcacagcg tgggttcacc gagccgcgtc gttgcccgtc gtgtcgcgcc agccgcaagg
     7321 ctgcccgcaa cgcgagtgg
//
LOCUS       DATAIW010000103         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_64466, whole genome shotgun sequence.
ACCESSION   DATAIW010000103 DATAIW010000000
VERSION     DATAIW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64466"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VHM48_03490"
     CDS             complement(<1..270)
                     /locus_tag="VHM48_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2754499.1"
                     /translation="MPSARRLLTAELLSIGSELTVGETRDTNAGDLARSLTGIGVEVA
                     RIQAVPDHLATVRDAFAAAAGRADLVVSTGGLGPTPDDLTREAIAA"
     gene            527..1180
                     /locus_tag="VHM48_03495"
     CDS             527..1180
                     /locus_tag="VHM48_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754500.1"
                     /translation="MHDLEQSGFSEFDRWLALAHRTRELLDAVLGDGVLPDGGADYLA
                     DLTLPHVEGVQAGFVGWLRSDSAGLAELRYLLARVGSMRVDGPTSHAERRAAAAEAAA
                     ELDLATRTGRRPAAALRVAEPWNLAALAHARLVLAMLPRLPDEDVRYPSGRRTYADIP
                     VPRGPAELSDRLEELERSLWRTATGHRPDPHDPAFRRAYGFFDAADRLGHRAFGSAA"
     gene            1682..2236
                     /locus_tag="VHM48_03500"
     CDS             1682..2236
                     /locus_tag="VHM48_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754501.1"
                     /translation="MLLLAAVALAAIVSALRPGIQASVGRAFALGLAVGVVVGVVLGL
                     NLTNRGWSALADAIVPAMDPAYRPLAVAVIGLAVVGGVLGLIAGAMAAGGGGAIGGLV
                     GGAVAGLFIGLLTAFAPGRRVGAAIGVTAGLITWMTAMGYGIARGGFDTDALKDRYWP
                     ARTIEATKETIEWARERMPLSRKS"
     gene            2197..2781
                     /locus_tag="VHM48_03505"
     CDS             2197..2781
                     /locus_tag="VHM48_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754502.1"
                     /translation="MGTRTDAALAQVVAARADLGEQVDRLEAAGRAAIDVPAKVRANP
                     AKAAGIAAGGAFLAVGGPKRLFRRARRAITGKEEELPSELLPKDVEKALRKIGTDGSK
                     VRGTLERDFAKYLDDRAKERKKEGVTAAIAAIALSALRPIAIRGGRQVAERMLDPNGP
                     KFAEQLERIRARRSAPDAADAETAAKGAPKDAGL"
     gene            3028..3115
                     /locus_tag="VHM48_03510"
     tRNA            3028..3115
                     /locus_tag="VHM48_03510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3061..3063,aa:Leu,seq:tag)
     gene            3211..>3583
                     /locus_tag="VHM48_03515"
     CDS             3211..>3583
                     /locus_tag="VHM48_03515"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX2754503.1"
                     /translation="MAVGHSDDVDAADAIAIAIDQCRAQLGGIVPQAGVLVAAFDSFD
                     PSLVAAVRDAFPGVALMGSTSSAEMSSTSGFLEDSVSLAMFASDAIDVTAGLGVGLDT
                     DVDAACRAAITEARSTTQREPK"
BASE COUNT          464 a         1328 c         1300 g          491 t
ORIGIN      
        1 cgccgcgatc gcctcgcggg tcaggtcgtc cggggtcggc ccgaggccgc cggtcgagac
       61 gacgagatcg gcccgcccgg ccgcggccgc gaaggcgtct cgaaccgtgg cgagatggtc
      121 gggcaccgcc tggatccggg caacttcgac gccgatgcca gtcaggctgc gggcgaggtc
      181 gccggcgttc gtgtcgcgcg tctcgccgac cgtcagctcg gagccgatgc tcagcagctc
      241 ggcggtcagg aggcggcgag cggagggcat gacggcagac tactagggca ggcgaatggc
      301 cgcgcatgcc gtcgctccgc gggccgcatc cgggtcgatc tcgcgcgacc aggcccccgt
      361 tcgcaacgcg ctcgacccgc gatcgcgacc gtccggcaga ggattccccc gagtaccatc
      421 ggccgagctg ccacggaacc ttcgcacggt gccgctcgag cgcgtcaccg tcgagtctcc
      481 tggtagcgat gctgtcggca tcggaacccg cggctgaggc ggccggatgc acgatctcga
      541 acagagcggc ttctcggagt tcgatcggtg gctcgcgctg gcccaccgga cgcgcgagct
      601 gctcgacgcc gtgctcggtg acggagtgct gcccgacggg ggcgccgact acctggccga
      661 cctgaccctg ccccatgtcg agggtgtcca ggccgggttc gtcggctggc tccggagcga
      721 ttccgcgggc ctggccgagc tgcgctacct gctcgcccgt gtcggctcga tgcgggtcga
      781 cggccccacg agccacgccg agcgtcgggc cgccgccgcc gaggctgccg ccgagctcga
      841 cctggcgacc cggacgggtc gtcgaccggc ggcggcgctg cgcgtcgccg aaccgtggaa
      901 cctggccgcc ctggcccacg cccggctcgt gctggcgatg ctgccgcgtc tccccgatga
      961 ggatgtccgc tacccgtccg gtcgccggac ctacgccgac atccccgtgc cgcgcgggcc
     1021 ggccgagttg tcggatcgcc tcgaggagct cgagcggtcg ctctggcgaa ccgcgaccgg
     1081 ccaccgaccg gacccgcacg atccggcctt ccgccgcgcc tacggcttct tcgacgcggc
     1141 cgaccgactc ggccatcgag ccttcgggag cgcagcctga ggtccgtcgc gcggacctga
     1201 cccgggccgc tttgccgttg cccgctatca tgggtgcggt cccccaggac atcgacggga
     1261 gcccaccctg gacgattcgc cgaatccgat ccccgcctcc gacccgccgc gcccacccgg
     1321 tgacggctcg accgccgatc cgaccgggtc ggtcccgccg gcaccgtcgt ccgcgtccgc
     1381 ggacccgggc cccgaggcgg gaccgggcgc cgccgcgccg ccgccgcccg caccggaggc
     1441 tcccggtctc cgagagcaga tcggcgcgac ccgcgagtcg gccacccggt tcgtcggcgc
     1501 ccacatcgag ctcggcaaag cggagttcgc cgatatcgca gacgcggcga agcgcgccgc
     1561 cgccttcgtc ggcatcgcga tcggggcagc catcgtcgcc gggctgatcg tcaccgtcgg
     1621 cctgcccctg ttcctcggcg aggcgatctt cggctcgatc ggctggggga tcctcctcgg
     1681 cgtgctgctc ctcgcggcgg tcgccctcgc ggcgatcgtc tccgccctca ggcctggcat
     1741 ccaggcgtcg gtcggccgcg cgttcgccct cggtctcgcg gtcggtgtcg tcgtcggggt
     1801 cgtcctcggg ctgaatctca cgaatcgcgg ctggtcggcc ctcgccgacg cgatcgtccc
     1861 ggccatggat ccggcctatc ggccgctcgc ggtcgcggtc atcgggctcg cggtcgtcgg
     1921 gggcgtcctc ggcctgatcg cgggagcgat ggcggcgggc ggcggcggag cgatcggcgg
     1981 gctggtcggc ggtgcggtcg ccggcctctt catcggcctg ctgacggcct tcgcgcccgg
     2041 tcggcgggtc ggcgccgcga tcggcgtcac cgccggcctg atcacgtgga tgaccgcgat
     2101 ggggtacggg atcgcccgcg gcgggttcga cacggacgca ctcaaggacc ggtactggcc
     2161 ggcccggacc atcgaggcca cgaaggagac gatcgagtgg gcacgcgaac ggatgccgct
     2221 ctcgcgcaag tcgtagcggc ccgcgcggac ctcggcgagc aggtcgaccg gctcgaggcc
     2281 gccggacggg cggccatcga cgtcccggcc aaggtccggg cgaacccggc caaggcggcc
     2341 gggatcgccg ccggcggcgc attcctggcc gtcggcgggc cgaagcgcct cttccggcgc
     2401 gcccgccggg cgatcaccgg caaggaggag gagctcccgt cggagctcct gccgaaggat
     2461 gtcgagaagg ccctccgcaa gatcgggacg gacggctcga aggtccgcgg cacgctcgag
     2521 cgcgacttcg cgaagtacct cgacgacagg gcgaaggagc gcaagaagga gggcgtgacg
     2581 gcggcgatcg ccgcgatcgc gctttcggca ctccgtccca tcgcgatccg cggcggccgc
     2641 caggtcgccg aacggatgct cgacccgaac gggccgaagt ttgcggaaca gctcgagagg
     2701 atccgcgccc gtcgctcggc gccggacgcg gcggacgcgg agaccgctgc caagggtgcc
     2761 ccgaaggacg ccggcctctg agatgctgcg gcactcgaga tcctgccaaa tgtccgtcac
     2821 gcggaccctc tgcacgaact cggacggttt gtgccctcga tctcgccacc cgttagcctc
     2881 tgccggctaa cgaacggacg tcgagcgacc cctgtcagcc tgggacggcg cctacggtcc
     2941 gcccgctgaa catctggcag gagcacccac ggatggcgct cgcgcgggag catgcacggc
     3001 ctccatcacc cgctagactc gcggcacggg cgagtggcgg aatggcagac gcgccggcct
     3061 taggagccgg tgtcgcgaaa gcgacgtgga ggttcgaccc ctctctcgcc taccaacggc
     3121 gcggccggac gcgacacgcc ctgccgcgcc taccaggctg gtcgcgcggt cggcaccgta
     3181 tgatcggctg cggaggcgac gatgctgacg atggcggtcg ggcacagcga tgacgtcgac
     3241 gcggccgacg cgatcgccat cgcgatcgac cagtgccggg cgcagctcgg cgggatcgtg
     3301 ccgcaggcgg gcgtgctcgt ggccgcgttc gacagcttcg acccgtccct cgtagcggcg
     3361 gtccgcgacg cgttccccgg cgtcgccctg atgggctcga cctcgtcggc cgagatgtcg
     3421 tcgacgagcg gcttcctcga ggactcggtg tcgctggcga tgtttgcctc ggacgcgatc
     3481 gacgtcaccg ccgggctcgg cgtcggcctc gacacggacg tcgatgccgc ctgccgggcc
     3541 gcgattaccg aggcacgctc gaccacccag cgcgagccga agg
//
LOCUS       DATAIW010000104        11965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_240506, whole genome shotgun sequence.
ACCESSION   DATAIW010000104 DATAIW010000000
VERSION     DATAIW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11965
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240506"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VHM48_03520"
     CDS             <1..247
                     /locus_tag="VHM48_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754504.1"
                     /translation="LRADDVPDAEGLVRRDLVGDVPALAAQAIAHRLGDLGDDASPRD
                     VAELLADGRDEGLGVRWRLVDGEGRPIVLDRSPRRSR"
     gene            complement(412..609)
                     /locus_tag="VHM48_03525"
     CDS             complement(412..609)
                     /locus_tag="VHM48_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754505.1"
                     /translation="MEPDDDLDAREAERIRRRDREAEAEQRDLMKPGMGKVFKQIQDA
                     QRKAAGDVAPRRPGRSVDRRP"
     gene            678..1349
                     /locus_tag="VHM48_03530"
     CDS             678..1349
                     /locus_tag="VHM48_03530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2754506.1"
                     /translation="MQALIFDLDGTLVDTVYAHVFAWQRALIDLGSPVDGWRIHRRIG
                     MSGGLFARAVAREIGRELSSDEAEALQRRHGEVFREILPERRPLPGAIELLAALRRGG
                     ITHGIATSGRRPEIDRSLEALDIPSETVVVERGDVLRAKPEPDLFLACQERLGVAARD
                     CYVVGDAVWDLLAARRAGMLSVGLLTGGYGEDELRAAGAFRVYRDAAELHESLDELGI
                     ALGPA"
     gene            1376..2197
                     /locus_tag="VHM48_03535"
     CDS             1376..2197
                     /locus_tag="VHM48_03535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2754507.1"
                     /translation="MAPLDAAIALGRAHGIRSAEPRVLKDGSNLLVHLDPAPVVIRVA
                     TFTGRIRRDPLPYLEREVRLATALVAEGAAVAPPSPLLPAGPHQIDGWSMTAWAFVEH
                     VAGAVPDAATAFVALDSLHDALRRVTIALPLLGPVTSDLDLAIDFALEVGLLSRADAD
                     ERRTRRDRLVAELLSVASDRQSLHGDAFPRNSLLTSRGVVWIDFEDCCLGPVAWDHAV
                     LIRNVADPTVERVLRERDGAAAIDAAIALREIQAHVWGTLHGARAEGRLDPDPDG"
     gene            complement(2211..3164)
                     /locus_tag="VHM48_03540"
     CDS             complement(2211..3164)
                     /locus_tag="VHM48_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2754508.1"
                     /translation="MRTIPELEPTAIREAHATIDPVFRDGPQYVHGGLSARLGVPVIV
                     KIETVNPIRSFKGRGTWVAVRALAASGAIGPDRAVICASAGNFGQGVAYAARALAIPA
                     LVFASCRANRGKVARMRALGAEVVEAGEDFDDARAEAETAAACGAGHLLVVGEDARIS
                     TGAATIALEITDAVAAGALPSPAIVSVPVGNGALINGVGAWLRSTLPTCRVVGVQAEG
                     AAAMTLSWRAGRPIDTASVATYADGIASRIAIPAAVELMIGRVDEMRIVSDDALRAAQ
                     IELTTELGITVEAAAAASWAGLTAGPRPDGPAIVIVTGSNV"
     gene            3307..4368
                     /locus_tag="VHM48_03545"
     CDS             3307..4368
                     /locus_tag="VHM48_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX2754509.1"
                     /translation="MPSAVHAPPPPLRAFAAASIILVVAVAGCATGGSAARAPGGEAG
                     ASLGWQDTLTGGPDRLDVATTVAPISSIARNIGGDRIRLRGIIPDATNSHTFEPAPSD
                     AQTLAKADLIIVNGLHLEQPTLDMAAAAKGPSAEIKQLGEGTIAEPDWLFDFSFPESA
                     GDPNPHLWMDVRYAIRYADLMRGWFGAADPPNRAAYDANYVAFESRLDQLDGLIRKGV
                     ATVPAQDRKLLTYHDSWAYWAREYGFTVIGAVQASDFSDPSPQEVARLIEQIRAEKVP
                     AVFGSEVFPSPVLAQIARESGATFVDQLSDDEPPGAAQGGEHTYLGMLKKDMQVMIGA
                     LGGDPTIFDPLVVTDTWRS"
     gene            4356..5288
                     /locus_tag="VHM48_03550"
     CDS             4356..5288
                     /locus_tag="VHM48_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX2754510.1"
                     /translation="MAIVTAAAVPAQGAVPIGEPLVQFERVTCGYDGPPVLRDVDLAI
                     ERGAFVGIVGPSGAGKTTLLRAIVGVVPRVEGRITVDGRPVGRHATGGVGWVPQLETV
                     DWNFPATVGEVVLMGRWAEHPWRAWATGEDRRRVDRLLERLGIGGLAGRHIRELSGGQ
                     QQRVFLARALIAEPALLLLDEPTSGVDLKTRDDILHLLAGLNADGITIVLTTHELNTV
                     AAHLPWVVCVNGAIVAEGDPDDIFTSAILGRTYGADLRVVRQDGLVLVADAAPHRLRD
                     ALRHRHGDVEHAHHEHQTHDHASAGGAPDHEHLP"
     gene            5285..6166
                     /locus_tag="VHM48_03555"
     CDS             5285..6166
                     /locus_tag="VHM48_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX2754511.1"
                     /translation="MTFLSEPLAFEFFRQGLIASGLVGALCGMLGVYIVLRRMSYIGH
                     GLSHSVFGGAVVGYVLALNFYLAAGLWGFLSALAINATARRRRIGADAAIGIVTTASF
                     AVGVALISKTRSFTRNFEAALFGNILGVTPVDLVVVGVVTLAVAAFIVLFYKRLLFVT
                     FDPEVAPIYGVNIGLVDTLFALAMAATIVASINVVGVTLIAATLVIPSTSARLLTDSF
                     GRMIGISTVIGALAGIVGMYLSYWLDVSSGATIVLLEAAIFLGCVVVTSVLRRRPAWV
                     GQPIPVLAHERPADDFE"
     gene            6163..6651
                     /locus_tag="VHM48_03560"
     CDS             6163..6651
                     /locus_tag="VHM48_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2754512.1"
                     /translation="MSGDRSTPGSVRPSIARPASANPWPDIRSELRRRGLRWTPQRKL
                     ILDVLGEARGHVTGSDIVERCRTREPETTPSTVYRTMAVLEDLGYLHHSHAADGREEF
                     HVLPVAEHAHLQCIGCGGSWEIDPAETSSLVAELERGRQFRVDVGHLTIAGRCATCAA
                     TD"
     gene            6898..7716
                     /locus_tag="VHM48_03565"
     CDS             6898..7716
                     /locus_tag="VHM48_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754513.1"
                     /translation="MTRVVRTGARAQVRARRLVIVVLAGLLLGIGSFALVSAATQPQR
                     TTPVYDPPVLAGQQLWGYCSGGFYARHGDTIVLTSTGHCTNEGTVAHDPDGTTVRGVF
                     GPAARDATCPYPGHWCAASDMNYLVVAEDRIPWGHLNVVDLGTAGYRIIPPGTRPLAC
                     ADIAIGDQVEIDGRNVYRSGTVAEKGENLHPEAQDGAYFPCMIASGIPAASGDSGGAV
                     LVRGIPAGVTSRSFGGALGFTPLAEGMAELGLDLCTTPDCGLVPPATDPTATAR"
     gene            7779..8216
                     /locus_tag="VHM48_03570"
     CDS             7779..8216
                     /locus_tag="VHM48_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754514.1"
                     /translation="MVVAGIVVGALAALVLSPSPSLPASRLIGWQLADGFLGTPRFDP
                     TTAQTTTVVPISVDWLTCAPKDESWLAPPAISYTSSSVTITMRSTDAFAATTTCGGSN
                     DSKGDISIMLDVGIPVAVHLSEPLRGRALFSGASSPPAARPYP"
     gene            complement(8282..9448)
                     /locus_tag="VHM48_03575"
     CDS             complement(8282..9448)
                     /locus_tag="VHM48_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2754515.1"
                     /translation="MRRTIGIGVVGMGWMGEAHSRSYRLVADRFHERGIDARLVACAD
                     SDPGRADAARERFGFERATTDWLDVVADPAVEAISITAPNDAHLAVIRAALAAGKHVL
                     CEKPVGRTPAETLEAAALARGTGLITFVGYNYRWAPVVQHARDLIAAGEIGELTHYRG
                     RFLNGYAKDPYGVLSWRFKAEQGYGTLSDLLSHAIDMAHLIAGPIADVVGNRRTFIAE
                     RPLPHPGGTHYDTAGPDDPRGEVTNEDYVGALVRFAGGAQGTLEACRVITGSQCDLGF
                     EVHGTNGALAWTFERMNELRFYRRNDANPADEGWTIELSGPAHPRHRAFNPGWGTGLG
                     FDDLKVIEAAEFLSAVAGGGPAAPSFEDAAAVARVQQAIASSWDSNGWERVAPA"
     gene            complement(9505..10506)
                     /locus_tag="VHM48_03580"
     CDS             complement(9505..10506)
                     /locus_tag="VHM48_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX2754516.1"
                     /translation="MLGGGVLVRGIAIFLAGFTLLALVGDLRGRTADLALWFVDIRDL
                     PRLVQAVLLGWFGVALAIWATAGAAGRLRRAAALVGCLVLAGFAARDVGRYASAVGSG
                     QVQPAAPVPLSLVLTAGLLGMAIWIWRDPGLTFDRPWRARSAVVGAAVVTALAFPVLQ
                     IGFFGTTDYRRPADAAVILGARVYADGVPSPLLADRIATGIELYRFGLVPVLVMSGGD
                     GTDGFNEALVMRDQAIAAGVDPSAILVDASGVNTDATVDHTLGLLAARLGSTANLRLI
                     AVSQAYHLPRIQLAFSGAGIDVLTVPAVDPVPISEMPLLVAREIPAFWLYYVRACLG"
     gene            complement(10613..11047)
                     /locus_tag="VHM48_03585"
     CDS             complement(10613..11047)
                     /locus_tag="VHM48_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2754517.1"
                     /translation="MPDNELEAGLAGEGAEWARDHIEHDVVGWLTTVAPDGRPQSSVI
                     SFLRDGATIFFYSRPDTPKLRNLAGSPLVSFHLQSDPYGDHALIVEGTATVDPSIPPS
                     DVHPTYRAKYREPLVHWSMDETETARDFSVPIRIHPARVRVS"
     gene            complement(11121..>11965)
                     /locus_tag="VHM48_03590"
     CDS             complement(11121..>11965)
                     /locus_tag="VHM48_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2754518.1"
                     /translation="ALLAELPDYRNGPYADLRKALLAEIEDTRVRASAVHRDSIAVRR
                     EGAAQIALVGPPNVGKSSLLQALSEIQIKTGDYPFTTLRPVPALTRIGGVLVQLVEIP
                     GLIEGAAEDRGGGRALLGVLRLADAIVYCGRADGDPGELAVVRAEVAAAGIVKPSLIA
                     ATRADEAGPGSIERLAAAAPDLEIVPVSILDEATLHAIREAIWRLTGLIRVRLRTNGS
                     TELEPIALEPGATVADVADRVHHDLAASFSGARVRGPSARFDDQRVGRDHVVADGDVV
                     EILR"
BASE COUNT         1690 a         4296 c         4209 g         1770 t
ORIGIN      
        1 cctccgcgcc gatgacgttc ccgacgccga gggcctggtc cggcgtgacc tcgtcggcga
       61 tgtgccggca ctggctgccc aggcgatcgc ccaccggctc ggcgatctcg gtgacgacgc
      121 gtcgccgcgg gacgttgcgg agctcctcgc ggacggccgg gacgaagggc tcggcgtgcg
      181 ctggcggctg gtagacggcg agggccgccc gatcgtcctc gatcgctcgc cgcgccgcag
      241 tcgctgagac cgcccgaagc ggacgcctgg cccccgccgg cgcgggctac acctggctca
      301 ggacggcagg tccccggtgg ccctggtcct ggcgtgcagt gttgctgccg cgcaaggacg
      361 cgattctacg ccggacgatc cgggtgaagg atccgatcgc cgggctcgac gtcagggtcg
      421 tcgatcgacc gagcggcccg ggcgccgtgg cgcgacgtcg ccagcggcct tgcgctgggc
      481 atcctggatc tgcttgaaga ccttgcccat cccgggcttc atcaggtccc gctgctctgc
      541 ctcggcttct cgatcgcgtc gccggattcg ctcagcttcg cgcgcgtcga gatcgtcgtc
      601 cggttccatg cgccgatcgt cgggcccggt cgcgaacgcg tcaagcccgc ttgcagcgat
      661 cgtgcgatcc ttcgccgatg caggccctga tcttcgacct cgacgggacc ctcgtcgaca
      721 ccgtctacgc tcatgtcttc gcctggcagc gcgccctgat cgacctcggc agcccggtcg
      781 acggttggcg gatccaccgc cggatcggga tgagcggcgg cctgttcgcg cgggccgtcg
      841 cccgcgagat cggacgggaa ctgagctcgg atgaggcgga ggccctccag cgccgccatg
      901 gcgaggtctt ccgcgagatc ctccccgagc gacggccgct gcccggcgcg atcgagctgc
      961 tcgccgcgct ccgccgaggc ggcatcaccc acgggatcgc gacatcgggc cggcgccccg
     1021 agatcgaccg gtcgctcgag gcgctcgaca tcccgagcga gactgtcgtg gtggaacgcg
     1081 gcgacgtcct ccgggcaaag cccgagccgg acctgttcct cgcctgccag gaacggctcg
     1141 gggtggccgc ccgcgactgc tatgtcgtcg gcgatgcggt ctgggacctg ctggcggcgc
     1201 gccgcgcggg catgctgagc gtcggcctcc tgaccggcgg ctacggcgag gacgagctcc
     1261 gggccgcggg cgcctttcgc gtgtatcgcg acgcggccga gctccacgag tcgctcgacg
     1321 agctcggcat cgcgctcgga cccgcctgag cggccgcgcc cggccgatgc gcgtcgtggc
     1381 cccgctcgat gccgcgatcg ccctcgggcg agcgcatggg atccggtccg cggagccccg
     1441 tgtcctgaag gacggcagca acctgctggt ccacctcgat ccggcaccgg tcgtcatccg
     1501 cgtggcgacg ttcacggggc ggatccggcg cgatccgttg ccgtacctcg agcgcgaggt
     1561 ccgcctcgcg accgcgctcg tcgcggaggg tgcggccgtg gcaccaccga gcccgcttct
     1621 gccggcgggg ccccaccaga tcgatggctg gtcgatgacc gcgtgggcgt tcgtcgaaca
     1681 cgtggccggc gccgtaccgg atgcggcgac cgccttcgtc gccctcgact ccctccacga
     1741 cgcgcttcga agggtaacga tcgcattgcc gctgctcgga ccggtgacgt cggatctcga
     1801 tcttgcgatc gacttcgcgc tcgaggtcgg cctgctttcc cgggccgatg ccgatgagcg
     1861 acggacgcga cgagatcgcc tcgtcgccga gctgctgtca gtggcctccg atcgccagtc
     1921 gctgcacggc gacgcctttc cccggaattc cctcctgacg agccggggcg tggtctggat
     1981 cgatttcgag gactgctgcc tcgggccggt tgcctgggat cacgcggtcc tgatccggaa
     2041 cgtggcggat ccaacggtcg agcgggtcct gcgcgagcgc gacggcgcgg ccgcgatcga
     2101 tgcggcgatc gccctgcgcg agatccaggc ccacgtgtgg gggacgctcc acggcgcccg
     2161 tgcggagggc cgcctcgacc cggacccgga cggctgacgc ggatgtcggg tcagacgttg
     2221 ctgccggtga cgatgacgat cgcgggacca tccggacgtg gcccggccgt gagcccagcc
     2281 caggacgccg ccgccgcagc ctccacggtg atcccgagct ccgtcgtcaa ttcgatctgg
     2341 gccgcccgga gcgcgtcgtc ggacacgatc cgcatctcgt cgacccggcc gatcatcaac
     2401 tcgacggcgg ccgggatcgc gatccgcgac gcgatgccgt cggcgtaggt cgccaccgat
     2461 gccgtgtcga tcggccggcc ggcccgccag ctgagggtca tcgctgccgc gccctcggcc
     2521 tggacgccca cgacgcgaca ggtcgggagg gtcgaacgga gccaggcgcc gacgccgttg
     2581 atcagcgcgc cgttcccgac cgggacggac acgatggcag gggagggcag ggcgccggca
     2641 gcgaccgcgt cggtgatctc gagggcgatc gtcgccgccc ccgtcgagat ccgggcgtcc
     2701 tcgccaacga cgaggagatg gccagcgccg cacgctgcgg ccgtctccgc ctcggcccga
     2761 gcgtcgtcga agtcttcgcc ggcctcgacg acctcggcgc cgagggcccg catccgggcg
     2821 accttgcctc ggttcgctcg gcacgaggcg aataccaggg cagggatcgc cagggcgcga
     2881 gcggcgtagg cgacgccctg gccgaagttg ccggccgagg cacagatcac ggcccgatcg
     2941 ggtccgatcg cgccgctcgc agccagcgcc cggaccgcga cccacgtccc gcggcccttg
     3001 aacgagcgga tcgggttgac ggtctcgatc ttgacgatga ccgggacccc gagccgggcc
     3061 gacagtccac cgtggacgta ttggggccca tcgcggaaga ccgggtcgat ggtcgcatga
     3121 gcctcgcgga tcgccgtcgg ttcgagctcc ggaatcgtgc gcacgctccg atggtaatcg
     3181 caccgaggcc gtgatccggg caccgccgtc gggcgtatcc actgttggca ccagtgcttg
     3241 ttacaacttg ttgcgataac ctgacctccg tctgcccgcc cgctcgaccc gcgaggttcc
     3301 gcccggatgc cgtccgccgt ccatgccccg ccgcccccgc tccgggcgtt cgcggccgca
     3361 tcgatcatcc tcgtggtggc cgtggccggt tgcgcgacgg gcgggtcggc ggcccgggca
     3421 ccaggcggcg aggccggcgc ctcgctcggc tggcaggaca cgctgaccgg aggcccggac
     3481 aggctcgatg tcgcgaccac cgtcgccccg atcagctcga tcgcgcggaa tatcggtggc
     3541 gatcggatcc ggctgcgggg gatcatcccc gatgccacga actcgcacac gttcgagccg
     3601 gctccgtcgg acgcgcagac cctggccaag gccgacctga tcatcgtcaa cggcctccac
     3661 ctggagcagc cgacgctcga catggccgcc gcggcgaaag gcccgtcggc ggagatcaag
     3721 cagctcggcg aaggcacgat cgcggagccc gactggctgt tcgatttcag ctttcccgag
     3781 tcggccggcg acccgaaccc gcatctgtgg atggatgtca ggtacgcgat ccgctacgcc
     3841 gacctgatgc gcggttggtt cggcgcggcc gacccgccga accgggcggc ctacgacgcc
     3901 aactacgtcg cgttcgagag ccggctcgac cagctcgacg ggctgatccg caagggcgtc
     3961 gccaccgtcc cggcccagga tcgcaagctc ctcacgtacc acgactcgtg ggcgtactgg
     4021 gcccgcgagt acggcttcac ggtcatcggc gcggtccagg cgtcggactt ctccgatccg
     4081 agcccacagg aggtcgcccg cctgatcgag cagatccggg cggagaaggt gcccgcggtc
     4141 ttcgggtcgg aagtgttccc gagccccgtt ctcgcccaga tcgcgcgcga gtccggggca
     4201 acgttcgtgg accagctcag cgacgacgag ccgcccggcg cagcgcaggg cggcgagcac
     4261 acctacctcg ggatgctgaa gaaggacatg caggtgatga tcggcgcgct cggtggcgac
     4321 cccacgatct tcgatccgct cgtcgtgacc gacacgtggc gatcgtgacg gcagccgcgg
     4381 ttccggcgca gggcgcggtg cccatcggcg agcccctggt ccagttcgag cgagtcacct
     4441 gcggctacga cggaccgccc gtcctccgcg acgtcgacct ggccatcgaa cgcggggcgt
     4501 tcgtcgggat cgtcgggccg tccggagccg gcaagacgac gttgctccgg gcgatcgtcg
     4561 gggtcgtgcc gcgggtagag ggccggatca cggtcgacgg ccggccggtc ggccggcatg
     4621 cgaccggcgg cgtgggttgg gtgccgcagc tcgagacggt cgactggaac ttcccggcga
     4681 ccgtcggtga ggtcgtcctg atgggtcgct gggccgagca tccgtggcgg gcctgggcga
     4741 cgggcgagga tcggcggcgg gtcgatcgcc tcctcgagcg gctcgggatc ggcgggctcg
     4801 cgggtcgcca catccgcgag ctgtccggcg gtcagcagca gcgcgtcttc cttgcccgcg
     4861 ccctgatcgc ggagccggca ctgctgctcc tcgacgagcc gacgagcggc gtcgacctca
     4921 agacccgcga cgacatcctc cacctgctcg ccggcctcaa cgccgacggg atcacgatcg
     4981 tcctgacgac ccacgagctc aatacggtcg ctgcccacct gccgtgggtc gtctgcgtga
     5041 acggggcgat cgtggccgag ggcgacccgg acgacatctt cacctcggcc atcctcggcc
     5101 ggacgtatgg cgcggatctc cgggtcgtcc gccaggacgg gctcgtcctc gtcgccgacg
     5161 ccgccccgca ccggcttcgt gatgcgctgc gccaccgcca cggcgacgtc gagcacgccc
     5221 accacgagca ccagacccac gaccacgcgt ccgccggcgg cgcgcccgat cacgagcacc
     5281 tgccatgacg ttcctgagcg agcccctcgc gttcgagttc tttcgccagg gcctgatcgc
     5341 ctccggcctg gtcggcgccc tgtgcgggat gctcggtgtc tacatcgtcc tgcgccggat
     5401 gagctacatc ggccacgggc tgtcgcattc ggtctttggc ggtgcggtgg tgggctacgt
     5461 cctcgccctg aacttctacc tcgccgcggg cctctggggc ttcctgtcgg ccctggcaat
     5521 caacgcgacc gcccgccgcc gccggatcgg ggcggacgcg gccatcggga tcgtgacgac
     5581 ggccagcttc gcggtcggcg tcgccctgat cagcaagacc cggagcttca cccgcaattt
     5641 cgaggcggcg ctgttcggca atatcctcgg cgtcacccca gtggacctcg tcgtggtcgg
     5701 agtcgtcacc ctggccgtcg ccgcgttcat cgtcctgttc tacaagcgcc tgctgttcgt
     5761 gaccttcgat cccgaggttg ccccgatcta cggcgtcaac atcggcctcg tcgacacgct
     5821 cttcgccctc gcgatggcgg ccacgatcgt ggcctcgatc aacgtcgtcg gcgtcaccct
     5881 gatcgcagcg accctcgtga tcccgtcgac ctcggcccgc ctgttgaccg actcattcgg
     5941 gcggatgatc gggatctcga ccgtgatcgg tgcgctggcg gggatcgtcg ggatgtacct
     6001 cagctactgg ctcgacgtgt cgtccggggc gacgatcgtc ctgctcgagg ccgcgatctt
     6061 cctcggctgc gtcgtggtca cgtcagtgct gcgccgccgt ccggcatggg tcggccagcc
     6121 gatcccggtc ctcgcccacg agcgacccgc ggacgacttc gaatgagcgg cgatcgctcg
     6181 acgccagggt cggtccgccc atcgatcgcg cggcccgcga gcgccaaccc gtggccggac
     6241 atccggtccg agctacgaag gcgcggcctg cgctggacac cgcagcggaa actcattctc
     6301 gacgtcctcg gtgaggcgcg cggtcacgtc accggcagcg acatcgtcga gcgctgccgg
     6361 acccgcgagc cggagacgac gccgtcgacc gtctaccgga cgatggccgt cctggaggac
     6421 ctcggctacc tccaccacag ccacgccgct gacggccgcg aggaattcca tgtcctgccc
     6481 gtcgccgagc acgcccacct ccagtgcatc ggctgtggcg ggtcgtggga gatcgatccc
     6541 gcggagacgt cgtcgctcgt tgccgagctc gagcgcggcc gccagttccg ggtcgacgtc
     6601 ggccacctga ccatcgccgg acgctgcgcg acctgcgccg cgacggactg acccgcggtg
     6661 cagacggccg acacggggtc gtcatccgtc gctgcggcca gccgccgatc gtcgagcgac
     6721 gcggcacgcc cgcgcgccga cgagccggtg aacgaacgcc cgccacacgc ggaggacctg
     6781 gagatcacgt cccgatcgag aaggcctgac catcaggggg ttccgtcgtc ggtgtgtgcc
     6841 actgatgatc ggatcaccgg gtcccgggtg cgacactccc ctgaacgagg gtggcaagtg
     6901 acacgagtgg tgaggacagg tgcgcgagcg caggtccgtg cccgtcgact cgtcatcgtt
     6961 gtcctggcgg gtctgctcct cgggatcggc tccttcgctc tcgtgagcgc cgcgacacag
     7021 ccgcagcgca cgaccccggt gtacgacccg ccggtgctcg ccggccagca gctctggggc
     7081 tattgctccg gcgggttcta tgctcgccac ggggacacga tcgtgctcac ctccaccggc
     7141 cattgcacca acgaagggac cgtcgcccac gacccggacg gcaccacggt acgtggcgtc
     7201 ttcggcccgg ccgcccggga tgccacctgt ccgtatccgg ggcactggtg cgcggcgtcc
     7261 gacatgaact acctggtggt cgccgaggac cgcattccat gggggcacct gaacgtcgtc
     7321 gacctcggca cggctggcta ccggatcatc ccgcctggca cgcggccgct cgcctgcgcc
     7381 gacatcgcga tcggtgacca ggtcgagatc gacggtcgca acgtctaccg gagcggaacg
     7441 gtcgccgaga agggcgagaa cctccacccc gaggcccagg acggcgccta cttcccgtgc
     7501 atgatcgcct ccggcattcc cgcagcttcc ggagactccg gcggcgccgt cctcgtgcgg
     7561 gggatcccgg cgggggtcac gagtcgcagc ttcggcgggg cgctcgggtt cacgccattg
     7621 gccgaaggca tggccgagct ggggctggat ctctgcacca cgcccgactg cggactcgtc
     7681 ccgcccgcga ccgatccgac ggcgaccgct cggtgagggc gacgcaaggc gcgtccgcgc
     7741 cgcggacgag gctccgtccc tacgcaatcg tcggggtcat ggtcgtcgcg ggcatcgtcg
     7801 tcggtgcgct cgcggccttg gtgctgagcc cgtcgccgtc gttgcccgcg tcacggctga
     7861 ttggctggca gttggccgat gggtttctgg ggacaccccg gttcgacccg acaaccgcgc
     7921 agaccacgac cgtggtcccg atctcggtcg actggctcac ctgtgcgccg aaggacgagt
     7981 cctggctggc gccgccggcg atcagttaca cgtcctcatc cgtgacgatc acgatgcgtt
     8041 cgactgatgc cttcgccgcc acgacgacat gtggtggtag caatgacagc aagggcgaca
     8101 tctcgatcat gctcgatgtg ggcatcccgg tcgcggtcca cctgagcgag ccactcaggg
     8161 gtcgagcgct gttcagtggc gccagctctc cgccggccgc gcgcccgtat ccctgagagc
     8221 cttggcgcct cgagaaacgc cgtcatcgat ttcgcgatga tccgatcgcg ggtcggtcgc
     8281 gtcaggccgg tgcgacccgc tcccagccgt tcgagtccca cgacgacgcg attgcctgct
     8341 ggacccgggc gacggcggcg gcgtcctcga agctgggtgc ggccggaccg ccgccggcaa
     8401 cggcactcag gaactctgcc gcttcgatga ccttgaggtc gtcgaagccg agcccggtac
     8461 cccaaccggg gttgaacgcc cggtggcgcg gatgggctgg cccgctcaac tcgatcgtcc
     8521 agccctcatc cgcggggttc gcgtcgttgc gccggtagaa gcggagctcg ttcatccgct
     8581 cgaacgtcca ggcgagcgcg ccgttcgtcc cgtgaacctc gaatccgagg tcgcattgcg
     8641 aaccagtgat cacccggcag gcctcgagcg tgccctgtgc cccgccggcg aaccggacga
     8701 gggcgccgac gtagtcctcg tttgtgacct cgccgcgtgg gtcgtcggga ccggcggtgt
     8761 cgtaatgggt cccgccgggg tgggggaggg gtcgttcggc gatgaacgtc cggcggttgc
     8821 cgacgacatc ggcgatcggc ccggcgatca ggtgggccat gtcgatggca tgggagagca
     8881 ggtccgacag cgtgccgtac ccctgctcgg ccttgaatcg ccacgacagg acgccgtagg
     8941 ggtccttggc atagccgttg aggaaccggc cgcggtagtg ggtcagctcg ccgatctcgc
     9001 ccgcggcaat gaggtcgcgg gcgtgctgga cgaccggcgc ccagcggtag ttgtagccga
     9061 cgaacgtgat caggccggtc ccccgagcca gcgccgccgc ttcgagcgtc tcggccggcg
     9121 tccgtccgac gggcttctcg cacaggacgt gcttgcctgc ggccagcgcg gcgcggatca
     9181 ccgcgagatg ggcgtcgttc ggggccgtga tgctgattgc ctcgaccgcc gggtcggcga
     9241 cgacatcgag ccaatcggtc gtcgcgcgct cgaacccgaa ccgctcccgg gctgcatcgg
     9301 cccggccggg gtcgctgtcc gcacaggcga caagccgggc gtcgatccca cgctcgtgga
     9361 agcgatcggc gacgagccgg tacgagcggc tgtgcgcctc gcccatccag cccatcccga
     9421 ccacgccgat cccgatcgtc cgtcgcatcc ggtgcgcctc ctcgaccaag cgtagcgatc
     9481 ctcgggagtg accggtgccc cgtttcagcc gaggcaggcc cggacgtaat agagccagaa
     9541 cgccgggatc tcccgggcga cgaggagggg catctcactg atcggcaccg gatcgacggc
     9601 cgggaccgtc aggacgtcga tccccgcacc ggagaaggcg agctggatcc ggggcaggtg
     9661 ataggcctgg ctgaccgcga tgaggcggag gttcgcagtc gaaccgagcc gtgcggcgag
     9721 cagcccgagg gtgtggtcga ccgtcgcgtc ggtattgacg ccgctggcgt cgacgaggat
     9781 ggcgctggga tcgacgcccg cggcgatggc ctgatcacgc atgacgaggg cctcgttgaa
     9841 gccatcggtc ccgtcgccac ccgacatcac gagaaccgga acgaggccga accggtacag
     9901 ctcgatgccg gtcgcgatcc ggtcggcgag cagcggcgag ggcacgccgt cggcgtacac
     9961 ccgcgccccg aggatcacgg ccgcatcggc gggacggcgg tagtcggtcg tcccgaagaa
    10021 cccgatctgc aggacgggga acgcgagcgc cgtgacgacc gccgcaccga cgacggcgga
    10081 tcgcgcccgc cacggccgat cgaacgtcag tccagggtcc ctccagatcc agatcgccat
    10141 ccccaggagc ccggccgtca ggacgagcga caggggaacg ggtgccgcgg gttggacctg
    10201 gcccgagccg accgccgacg cgtagcgccc cacgtcgcgt gcagcaaacc ccgcgagcac
    10261 gagacagccg acgagcgccg cggctcgccg cagtcgaccg gccgcgcccg ctgtcgccca
    10321 gatggcgagg gcgactccga accaaccgag cagcaccgcc tggaccaggc gcggcaggtc
    10381 gcggatgtcg acgaaccaga gggccaggtc ggctgtccga ccacgcaggt cgccgacgag
    10441 cgcgaggagc gtgaacccgg cgaggaagat cgcgatcccc cggacgagga ctccgccgcc
    10501 cagcatcgcc agtcgtcgca cggctggcag acgcgcggct gccaccgcat gtggtgcgga
    10561 ccgtcggacg gagcggcgga ccggcgacgg cacggcggac gaggccgccg ggtcagctca
    10621 cccggacccg ggccgggtgg atccggatcg gaaccgagaa gtctcgggcc gtctccgtct
    10681 cgtccatgga ccaatggacg agtggctcgc ggtacttggc gcgatacgtc gggtgtacgt
    10741 ccgacggcgg gatcgacgga tcgacggtgg cggtgccctc gacgatcagc gcgtgatctc
    10801 cgtacggatc cgattggaga tggaacgaga ccagcggcga gccggccagg ttccgcagct
    10861 tcggcgtgtc cggccggctg tagaagaaga tcgtcgcacc gtcgcggagg aacgagatca
    10921 ccgacgactg cggtcgcccg tcgggggcga cggtcgtcag ccagccgacc acgtcgtgct
    10981 cgatgtgatc gcgagcccat tcggcgccct cgccggccag gcccgcttcg agctcgttgt
    11041 caggcatcga tccgttcctc catccgttcc gccgtgccgg ctcgtcgccg cagcgccgat
    11101 catccagcct ggccgtcggg tcagcgcagg atctcgacga cgtcgccgtc cgcgacgaca
    11161 tgatcccgac cgacgcgctg gtcgtcgaat cgcgccgatg gcccccggac ccgcgctccg
    11221 ctgaacgagg cggcgaggtc gtggtggacc cggtcggcga cgtcggcgac ggtcgcgccg
    11281 ggctccaggg cgatcggctc cagctcggtc gatccgttcg tccgaagacg cacccggatc
    11341 agcccggtca accgccagat cgcctcgcgg atcgcgtgga gcgtcgcctc gtcgaggatg
    11401 gacaccggca cgatctcgag gtcgggagca gcggcggcga gccgctcgat cgaacccggg
    11461 ccggcctcgt ccgcgcgcgt ggcggcgatg agggagggct tgacgatgcc ggcggcagcc
    11521 acttcggccc ggacgacggc aagctcgccc gggtcgccgt cggcccgacc gcagtagacg
    11581 atcgcgtcgg ccaggcggag cacgccgagg agcgcccgcc cgccgccgcg gtcctccgcc
    11641 gcgccctcga tcaagcccgg gatctcgacg agctggacga ggaccccgcc gatccgggtc
    11701 agggccggga ccggtcgcag ggtcgtgaac gggtagtccc cggtcttgat ctggatctcc
    11761 gacagcgcct ggagcaggga tgacttgccg acgttcggcg gtcccaccag cgcgatctgg
    11821 gccgctccct cgcgccggac cgcgatcgag tcacggtgga ccgcgctcgc ccggacacgc
    11881 gtgtcctcga tctcggcgag gagcgccttg cgaaggtccg cgtacggacc gttgcgatag
    11941 tcgggcagct cggcgaggag ggccc
//
LOCUS       DATAIW010000105         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_181032, whole genome shotgun sequence.
ACCESSION   DATAIW010000105 DATAIW010000000
VERSION     DATAIW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181032"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VHM48_03595"
     CDS             complement(<1..633)
                     /locus_tag="VHM48_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2754519.1"
                     /translation="MTTESERTRIERYDPTSIEPRWQQRWAEIGLYRTDLHDDSRPKF
                     YLLTMYPYPSGDIHIGHWYIITPTDAIARFHRMHGENVFLPIGFDAFGLPAENAAIKN
                     NINPRDWTMRNIDNMRRQLRSMGATFDWESEVVTADPAYYRWNQWFFLQFLKAGLAYR
                     STSPVDWCPNDGTLAREQVEGVDRHCWRCGAKVEKRDLAQWYLKVTKYADE"
     gene            complement(720..795)
                     /locus_tag="VHM48_03600"
     tRNA            complement(720..795)
                     /locus_tag="VHM48_03600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Ala,seq:ggc)
     gene            complement(909..2330)
                     /locus_tag="VHM48_03605"
     CDS             complement(909..2330)
                     /locus_tag="VHM48_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754520.1"
                     /translation="MLDHSLEQKLRAALRTEGDDLAFTITSEELERRLALRRRGGLSP
                     LASLGLAAAVGVGMLGLAAVVGGWFDPRPAVGPLPSRSAVVVQASPSATPSPAPSGGP
                     LALPSLDELLGTIDPARVVRAEAVGPNGGLGDQLGAFNSPIEPNVVTFDPVATSGTYY
                     VLAVCAGDGRVVMIVVRADGQSIEEVPITCDGARSLRQMGLEAGSSLQVTSVGHSGWR
                     VVLLAPDRPAPHAATIDRGGVVPEGADVALEAESRTLEPDYTDILTGGGIQSRTEVGS
                     LATRTRYDVYISCAGPTSVRYAFGGRRSSDEPVDPGVLIEYVAGEVECDGTLQHDVIE
                     LTLGGGDVSVTAEDRTVWRIVVTQEKPPIASAPDEGGWTLSSGGGPNLDINGQLEDAT
                     MAGNAAGGDIRIVVSCSGATTLDLIVDTGPVVGQRVDHLSVDCREGFGTVEKVYAKAG
                     PYIEVTSDPHGVRTWIAITVQTR"
     gene            complement(2323..2892)
                     /locus_tag="VHM48_03610"
     CDS             complement(2323..2892)
                     /locus_tag="VHM48_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754521.1"
                     /translation="MTADPPEGQMTVEALRRRRPQTLAELLDAHGREIQAVAFLILRD
                     RSAAEDVTIETLLTAFEKGGSIRDGSAVRAWLLKVAANKALAIRRRGARLVNLDQLPD
                     RAAPGDLAGEAASHIALLAGVADLPPAMRAAVVLRYYSDLPVEEVAAALGKSPNTIKA
                     QLQTALDRLRHSLADPAADAATPREARHA"
BASE COUNT          467 a         1159 c         1177 g          483 t
ORIGIN      
        1 ctcgtcggcg tacttggtga ccttgagata ccactgggcg agatcgcgct tctcgacctt
       61 cgccccgcac cgccagcagt ggcgatcgac gccttcgacc tgctcgcgag ccagcgtccc
      121 gtcgttcggg caccagtcga ccggcgaggt cgaccggtag gcgagtccgg ccttcaggaa
      181 ctggaggaag aaccactggt tccagcggta gtacgccggg tcggcggtga ccacctccga
      241 ctcccagtcg aacgtcgctc ccatcgaccg cagctggcgg cgcatgttgt cgatgttgcg
      301 catcgtccag tcgcgcgggt tgatgttgtt cttgatcgcg gcgttctcgg ccggcaggcc
      361 gaaggcatcg aagccgatcg gcaggaacac gttctcgccg tgcatccggt ggaagcgggc
      421 gatcgcgtcg gtcggcgtga tgatgtacca gtggccgatg tggatatcac cggacgggta
      481 cgggtacatc gtcaacaggt agaacttcgg ccgcgaatcg tcgtggaggt ccgtccggta
      541 caggccgatc tccgcccagc gctgctgcca gcggggctcg atcgaggtcg ggtcgtagcg
      601 ctcgatccgc gtgcgttcgc tctcggtcgt cacggtcgtc tcacggctcc cggatggacg
      661 acgacccccg gccgctccgg cagggggtcg tggatggtcg gctctgaccg gcggggaatt
      721 ggtggagcta agggggctcg aacccctgac cttctgaatg ccattcagac gctcttccag
      781 ctgagctata gccccgcgcc ggagcgggaa cgatagcaca acctgagcgt ggatcggccg
      841 ccggccccga gggttccggc ggccgaagga gccgccggtg gcggccgccc cgatcggacc
      901 gcgccggctc agcgagtctg gaccgtgatc gcgatccagg tccggacgcc gtgcggatcc
      961 gacgtcacct cgatgtacgg gccggccttc gcatacacct tctcgaccgt cccaaagccc
     1021 tcgcggcagt cgacgctcag gtgatcgacg cgctggccga cgaccgggcc ggtgtcgacg
     1081 atcaggtcga gtgtggtcgc gcccgagcac gacacgacga tccggatgtc gccgccggcc
     1141 gcgttgcccg ccatcgtggc gtcctcgagc tgcccattga tgtcgaggtt cgggccaccc
     1201 cctgacgaca gggtccagcc gccctcgtcc ggcgccgacg cgatcggggg cttctcctgg
     1261 gtcacgacga tccgccagac ggtccggtcc tcggcggtca ccgagacgtc gccgccgccg
     1321 agggtcagct cgatcacgtc gtgctggagc gtgccgtcgc actcgacctc gccggcgacg
     1381 tattcgatca acacgcccgg gtcgacgggc tcgtcggacg atcgccgccc gccgaacgcg
     1441 taccggacgc tcgtcgggcc ggcgcagctg atgtagacgt cgtagcgggt ccgggtggcg
     1501 agcgagccga cctcggtccg gctctggatc ccgccaccgg tcaggatgtc ggtgtagtcg
     1561 ggctcgaggg tccggctctc ggcctcgagg gcgacgtccg ccccctccgg cacgacgccg
     1621 ccgcgatcga tggtcgccgc atgcggcgcc ggtcgatcgg gcgcgaggag gacgacccgc
     1681 cagccgctgt ggccgacact tgtaacctgg agcgagctgc ccgcctccag gcccatctgg
     1741 cggagtgacc gggcgccgtc gcaggtgatc ggcacctcct cgatggattg gccatcggcg
     1801 cgcacgacga tcatgacgac gcgcccgtcg ccggcgcaga ccgcgaggac gtagtaggtg
     1861 ccgctcgtcg ccaccgggtc gaacgtgacg acgttcggtt cgatcggcga gttgaatgcg
     1921 ccgagctgat cgccgaggcc gccgttcggc ccgacggcct ccgcccggac gacccgcgcg
     1981 gggtcgatcg tgccgagcag ctcgtcgagc gacggcaggg cgagcgggcc gcccgacggc
     2041 gcggggctcg gcgtcgcgct cggcgaggcc tgaacgacga ccgcggatcg gctcggcaac
     2101 ggcccgaccg ccggccgcgg atcgaaccac ccgccgacga cggccgccag gccgagcatg
     2161 ccgacgccga ccgcggccgc cagcccgagg ctcgccaacg ggctcagccc gccccgccgc
     2221 cggagcgcga ggcgccgctc gagctcctcg gacgtgatcg tgaacgccag gtcgtcgccc
     2281 tcggtccgga gcgcggcccg gagcttctgc tcgagcgaat ggtcaagcat gtcgggcctc
     2341 ccggggcgtg gcggcgtcgg cggcaggatc ggccagcgag tggcgcagcc gatcgagcgc
     2401 ggtctggagc tgagccttga tcgtgttcgg gctcttgccg agggcggcgg cgacctcctc
     2461 caccggcagg tcgctgtagt agcggagcac gaccgcagcc cgcatcgccg gcggcaggtc
     2521 ggcgacgccg gcgaggaggg cgatgtggct ggccgcctcg ccagcgaggt cgcccggcgc
     2581 cgcccggtcc ggcaactggt cgaggttgac gagccgcgcc ccacgccgcc ggatcgcgag
     2641 cgccttgttc gccgccacct tgaggagcca cgcccggacg gcggacccgt cgcggatcga
     2701 gccgcccttc tcgaaggcgg tgaggagcgt ctcgatcgtg acgtcctcgg ccgccgatcg
     2761 gtcgcgcagg atgaggaacg cgaccgcctg gatctcgcgg ccatgggcgt cgaggagctc
     2821 ggccagggtc tgcggtctgc ggcgacggag cgcctcgacg gtcatctgcc cctcgggtgg
     2881 gtcggcggtc atcgtctgat gacgattccg ctctcaccga tcagatgcac gggcggggcg
     2941 gttcgggcta gtgtgccggg ccggatcgat cgccgccgtc gcggacccgc ggatccaccc
     3001 tatccgcgcc gatccacgcc ggaccacccc gcagccctcc agcgggccac cccgaacggc
     3061 gtcgaaaggg tgcgcccgtg caacgaggtc atgggtaggt cgagctatgc tgtccggtac
     3121 cttcggatgc gagcgccctg ccacagcgcc gccgcgaagg cccagaccgc atcgtcctcg
     3181 cgtcactgca ccaggtcagg gaggccccgc tcatgtcgct gctccaacgg gtggaacggg
     3241 cgcagcaggc actcgaacgc aagaacggca gcgaggtcga gctgtc
//
LOCUS       DATAIW010000106         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_94288, whole genome shotgun sequence.
ACCESSION   DATAIW010000106 DATAIW010000000
VERSION     DATAIW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94288"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VHM48_03615"
     CDS             <1..1056
                     /locus_tag="VHM48_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754522.1"
                     /translation="LAFGVTKALANLAAGALADRYGRKPVLVAGWLFGLPVPLLLIWA
                     PDWGWVIVANVLLGINQGLAWSSTVIMKIDLVGPARRGLAMGLNEAAGYGAVALTALA
                     TGFIAAQAGLRPAPFFLGLAFAGLGLGVSVLFVRETREHVALEAAGKPGKPDLPWRTV
                     FARTSFRDPSLSAASQAGLVNNLNDGMAWGLLPLLYASHGLAVAEVGILAAAYPAVWG
                     LGQIGTGALSDRIGRKGLIVGGMLLQAGAIGVIAVASTFAVWLLAAALLGLGTAMVYP
                     TLLAAVADVAAPSWRGAAVGVYRLWRDLGFAAGAVVAGVLADRAGMPFAIGVIAALTA
                     VSGIVVLVRMRETRPIA"
     gene            1098..1493
                     /locus_tag="VHM48_03620"
     CDS             1098..1493
                     /locus_tag="VHM48_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2754523.1"
                     /translation="MAERGTVNDLLARSRSRIERLEPRAALAAAEAGAIVIDTRCAEQ
                     RRETGTIPGSVHVPLSVLYWRLDPASGFEDPRLADLERQVILVCAHGYSSSIAAATLR
                     DLGFSRASDLTGGFEAWQAAGLPIQAVAD"
     gene            1549..1899
                     /locus_tag="VHM48_03625"
     CDS             1549..1899
                     /locus_tag="VHM48_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2754524.1"
                     /translation="MKVLLILNDGPYGTERSYNGLRLALALAKSPDATVRVFLMADAI
                     ACAKSGQTTPEGYYNIGRMLRGLTGRGVEVGTCGSCMDARGYADADLADGVCRSSMAQ
                     LAAWTLEADKVVTF"
     gene            1977..3806
                     /locus_tag="VHM48_03630"
     CDS             1977..3806
                     /locus_tag="VHM48_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2754525.1"
                     /translation="MPPTPSKRPGMGAIPFADDAGRGVAFRVWAPHATSAAVVGTFND
                     WEARRNQLARDDDGKAETWSADAAAARAGDEYRFVLQTPAGERNRIDPRARRLTNSVG
                     NGVIYDPAAFDWGDAEFRQPPWNDLVVYELHVGTFSEGLHGKPGTLDGVRRRLPYLRE
                     LGVGAIQLMPPFEFAGDRSWGYNTAYPYAVESTYGSPDDLKALVRDAHATGIAVFLDV
                     VYNHLGPSDLDIWQFDGWSQDGKGGIYFYEDVRSTTPWGETRPDYGRTEVRAFLRDSA
                     IQWLDEFHVDGLRWDATAWISSIYGGGSSAPDRIEDGWRFMADVNAEIAARYPGRLTI
                     AEDLRSDLAIIRPSAEGGAGFGAQWDGAFVYFVRTALVASNDAHRDILSVVGVIDGGD
                     IDSDAFARVIYTESHDEDANGSARVPEEIWPGYADSWASKKRATLGSAIVLTSPGIPM
                     LFQGQELVEGSWFDDEDGLDWTLRHRHAGLLRLHRDLISLRRNAADVSRGLRGEHVTV
                     HHVNEEAKVLGWHRWLEGGPRDDVIVLANFSTDTFPDYHVGVPRRGRWRVRFNSAWDG
                     YDPEFETVVSLDADSEARAHDGMAQRIAVGLGPYSVVILSQDE"
BASE COUNT          567 a         1471 c         1408 g          638 t
ORIGIN      
        1 ctggccttcg gcgtgaccaa ggcgctcgcc aacctcgctg ccggcgcgct ggccgaccgc
       61 tacggccgga agcccgtgct cgtcgccggc tggctgttcg gactgcccgt gccgctgttg
      121 ctcatctggg cgccggactg gggctgggtc atcgtcgcca acgtcctgct cggcatcaac
      181 cagggcctgg cctggtcgag cacggtgatc atgaagatcg acctggtcgg tcccgcccgt
      241 cgtggattgg cgatgggcct caacgaggcg gcgggctacg gcgcggtcgc cctgaccgcc
      301 ctcgccaccg gcttcatcgc ggcccaggcc gggctccgtc cggcgccctt cttcctcggg
      361 ctggcctttg cgggtctggg cctcggcgtc tcggtcctct tcgtgcgcga gacccgcgaa
      421 cacgtggcac tcgaggcggc cggcaagccc ggcaagccgg acctgccctg gcggaccgtc
      481 ttcgcccgga cgtcatttcg cgacccgtcg ctgtcggccg cgtcgcaggc cggcctggtg
      541 aacaacctca acgacggcat ggcctggggt ctgctgccgc tgctctacgc ctcgcatggc
      601 ctggccgtcg ccgaggtcgg catcctggcg gccgcctacc cggcggtctg gggcctcggc
      661 cagatcggga ccggagcgct gtcggatcgg atcggacgga aagggctcat cgtcggcggg
      721 atgctgctcc aggcgggtgc catcggggtg atcgccgtcg catcgacctt cgcggtctgg
      781 ctgctcgcgg cggcgctgct cggtctcggc acggcgatgg tctacccgac cctgctcgcc
      841 gcggtcgcgg atgtcgcggc accctcgtgg cgcggcgccg cggtcggtgt gtaccgcctg
      901 tggcgggacc tgggtttcgc ggccggcgcc gtcgtcgccg gcgtcctcgc cgaccgggcc
      961 ggaatgccct tcgcgatcgg cgtcatcgcg gcgctgaccg ccgtctcggg cattgtcgtt
     1021 ctcgtccgaa tgcgcgaaac gcgccccatc gcttgacccg aacgttgaca atcgctcgaa
     1081 acaggaggat cgcgcgcatg gccgaacgag ggaccgtcaa cgacctactc gcgaggtcac
     1141 gctcacggat cgagcggctg gaaccgcgag ccgctctcgc cgctgcggaa gcgggcgcga
     1201 tcgtgatcga tacgcgctgc gcggagcaac ggcgtgagac cggcaccatc ccggggtcgg
     1261 tccacgtgcc gctctcggtc ctgtactggc gactcgatcc cgcatcgggc ttcgaggacc
     1321 cccgcctcgc cgatctcgag cgacaggtga tcctcgtgtg cgcccacggc tactcgtcga
     1381 gcatcgccgc cgccaccctg cgggacctcg ggttttcccg cgcttcggat ctcaccggtg
     1441 gcttcgaggc gtggcaggca gccgggttgc caatccaggc cgtagcggac tgaccttcgg
     1501 gccgaatggc cggtgggcac cgcggcgcgg cccgccgatc ctcggctcat gaaggtgctc
     1561 ctgatcctca acgacggccc ctacggcacc gagcgctcgt acaacggtct tcgtctcgcc
     1621 ctggccctgg ccaagtcgcc cgacgcgacg gtccgggtct tcctgatggc cgacgcgatc
     1681 gcctgcgcga agtcgggcca gacgacaccc gagggctact acaacatcgg tcggatgctc
     1741 aggggtctga ccggcagggg agtcgaggtc gggacctgcg gctcgtgcat ggacgctcgc
     1801 ggctacgccg acgccgatct cgccgacggc gtctgccgct cgtcgatggc gcagctcgcc
     1861 gcgtggacgc tggaggccga caaggtcgtc accttctgac caccgaggcg tctctaccac
     1921 gtggcgggca gccggacccg ccaatccgga cggccgaggt cgtgtagcct cgcttcgtgc
     1981 caccgacacc ctcaaagcgc cccggcatgg gcgcgattcc gttcgccgac gacgccggtc
     2041 gcggcgttgc gttccgggtc tgggcgcccc atgcgacgag cgcagcggtc gtcgggacgt
     2101 tcaacgactg ggaggcgcgg cgcaaccagc tcgcccgcga cgacgatggc aaggccgaga
     2161 cgtggtccgc cgacgcggcc gcggcacgcg cgggcgacga atatcgcttc gtcctccaga
     2221 ccccggccgg cgagcgcaac cggatcgacc cgcgcgcccg gcggctgacc aactcggtcg
     2281 gcaacggcgt gatctatgac cccgcggcgt tcgactgggg tgacgccgag ttccgtcagc
     2341 cgccatggaa cgacctcgtc gtctacgagc tccacgtcgg gacgttcagc gaggggctgc
     2401 acggcaagcc cggaaccctc gacggcgtcc gccggcgcct gccgtatctc cgcgagctcg
     2461 gtgtcggggc gatccagctg atgccgccgt tcgagttcgc cggcgaccgc tcgtggggct
     2521 acaacacggc ctatccgtac gcggtcgaga gcacctacgg gtcgcccgac gacctcaagg
     2581 cgctggtccg cgacgcgcat gcgaccggga tcgcggtgtt cctcgacgtc gtctacaacc
     2641 acctcggccc gtccgatctc gacatctggc agttcgacgg gtggtcgcag gacggcaagg
     2701 gcgggatcta cttctacgag gacgtccgct cgacgacgcc gtggggcgag acccggccgg
     2761 actacggccg aaccgaggtc cgggcgttcc tgcgcgacag cgcgatccag tggctcgacg
     2821 agttccacgt cgacggcctc cgctgggacg ccacggcgtg gatctcgagc atctacggcg
     2881 gcggcagcag cgcgccggat cggatcgagg acggctggcg gttcatggcc gacgtcaacg
     2941 ccgagatcgc cgcgcgctat ccgggccggc tgacgatcgc cgaggatctc cgctcggacc
     3001 ttgccatcat ccgcccatcg gccgaaggcg gcgccggctt cggggcccaa tgggacggtg
     3061 ccttcgtcta cttcgtccgg acagccctgg tcgcgagcaa cgatgcccat cgggacatcc
     3121 tgtcggtcgt gggcgtgatc gacggcggcg acatcgactc cgatgccttc gcccgcgtga
     3181 tctacaccga gtcccacgac gaggatgcca acggctcggc ccgcgtcccc gaggagatct
     3241 ggcccggcta cgccgactcg tgggcgtcga agaagcgggc aacgctcggc agcgcgatcg
     3301 tcctgaccag ccccgggatc ccgatgctgt tccagggcca ggagcttgtg gaaggctcgt
     3361 ggttcgacga cgaggacggc ctcgactgga ccctccgcca ccgccacgcc ggactccttc
     3421 gcctccatcg cgacctgatc tcgctgcgcc gcaacgcggc ggacgtctcc cgcgggctgc
     3481 gcggcgagca cgtgacggtc caccacgtca acgaggaggc gaaggtcctc ggctggcacc
     3541 gctggctgga gggcggaccg cgcgacgacg tgatcgtcct cgccaacttc tcgacggata
     3601 cctttcccga ctatcacgtc ggcgttccgc gacggggtcg ctggcgggtc cgcttcaaca
     3661 gcgcctggga cggctacgac ccggagttcg agaccgtcgt ctcgctcgac gccgacagcg
     3721 aagcccgagc ccacgatggg atggcccagc ggatcgcggt cggcctcggg ccgtactcgg
     3781 tggtgatcct ctcacaggac gagtaggggc cgcgccgcgc gccccgcgtc gccggtccgc
     3841 tctcgccccc gcccgtcgcc gattcctgac cacatattca tggagggact gttgggacgt
     3901 gtatcgaacg ttccgcccga tcacgccggg taggcgttag tcgaatccgg ctaacttcga
     3961 ccggaaatca gcgcgctgac gcgctccgag cgccggaatc tggtcgaaga gtccaccggc
     4021 gaatatccgg ccaggtagca cgggcccgcc gagcggggcg aaggggcccg gcgcgccgtc
     4081 actc
//
LOCUS       DATAIW010000107         8934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_4637, whole genome shotgun sequence.
ACCESSION   DATAIW010000107 DATAIW010000000
VERSION     DATAIW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4637"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHM48_03635"
     CDS             <1..259
                     /locus_tag="VHM48_03635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX2754526.1"
                     /translation="RIEAWPSSAILLDRATPVYEDFAGWEEPIHEVRALSDLPDAARR
                     YVTALEEHAGAPIVLVSVGPERTQTIERAWRPMRHRVVAGP"
     gene            453..1778
                     /gene="purD"
                     /locus_tag="VHM48_03640"
     CDS             453..1778
                     /gene="purD"
                     /locus_tag="VHM48_03640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2754527.1"
                     /translation="MTHLVQPTRILVVGGGGREHALAWKLAAEPGVNEVVVAPGSDAI
                     AREPRVRVVPGLDPLDPAAVVALARKRAIELVVIGPEAPLAAGVADALGTAGVPVFGP
                     TAAAARIETSKTFCHEIAAEAGVPMANAATFRASDSAEARAWARDEADRAGSRGVVVK
                     ADGLAAGKGVVVCDTAAEAGAAIDALVAGGTERILVEERLTGPEATVIAICDGRYAVA
                     LPAARDHKRLGDGDTGPNTGGMGGYSPLPDLDDDAVAAILATVHEPILAALGRRLLDY
                     RGFLYAGLMLTDDGPRLLECNARLGDPEAQVILPRLAGPLGPLLLTAARGRLESGARI
                     PVLPGAAVGIVLAAAGYPAAPIRGVAIDGLAVNPDGLVFHAGTRRDGDGSWRVDGGRV
                     LCVVGRGPDLAAARVIAEVAADSISWPGVQRRHDIGAAARPSEPVAAAR"
     gene            1775..3097
                     /gene="purB"
                     /locus_tag="VHM48_03645"
     CDS             1775..3097
                     /gene="purB"
                     /locus_tag="VHM48_03645"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2754528.1"
                     /translation="MIRRYTLPEMGSIWTEKARFEQMLRVELAVSRAQAARGVVPADA
                     LANIEARATVDPERIAEIERTTDHDVIAFVSQVAETVGPDGRYLHLGLTSSDVVDTAL
                     ALQLRDAGELLIRDCDRLLAVLIARARSEADTLMMGRTHSVHAEPITFGLKLAGWAFE
                     VDRGRRRLATAVDEIGTGKISGPVGTYSHLGPDVEAEVLAELDLHADPSSTQIVQRDR
                     HAALLTTIAILGGSLERFATEIRNLQHTEIGEVMEAFKPGQKGSSAMPHKRNPILAER
                     IAGLARLLRGYAQTALENQPLWHERDISHSSAERVILPDATIVLDYLLVKTAGLVEGL
                     VVRRERMLENIERGHGLHASSRVLGTLVERGGMSREAAYAIVQRAALRAADERSPLRE
                     LLALDPEVAKRMSLADLDACFDDAAHLRHVPAVIARLDSLEAEIHAAR"
     gene            3084..4193
                     /locus_tag="VHM48_03650"
     CDS             3084..4193
                     /locus_tag="VHM48_03650"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2754529.1"
                     /translation="MPPASPLADSFLRSGKVRDLYELPDHRMVLVASDRISAFDVVLP
                     SEIPDKGRVLTGISRFWFAETTSIVRNHLLATDTTQIGDAFAARGISSEAIGPLDRLR
                     GRVMICRPAIVLPIEAVVRGYLAGSGWKEYQQTGTVCGIPLPAGLRESDRLPEPIFTP
                     ATKAPIGEHDENISFDGMIDHLAGDAWVLGGAREHAPALAERVREISLALYRFGAAVA
                     ERKGVTLADTKFEFGLGGSGDPDDLEPVGPPQEQTGELRGEGWLALSATGRLESNLML
                     IDEVLTPDSSRFWDAADYEPGRSQASFDKQYVRDWLETQPWDKTAPGPELPADVVTGT
                     RARYVEAFERITGASFQRYLDADVIGVDGSDGQKA"
     gene            4196..4498
                     /gene="purS"
                     /locus_tag="VHM48_03655"
     CDS             4196..4498
                     /gene="purS"
                     /locus_tag="VHM48_03655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2754530.1"
                     /translation="MDTRFAVNVSPKPGILDPQGRAVEGSLDHLGIAGVSAVRVGRRV
                     ELTVSAADDAAARAVVERLAAELLSNPLIEAYDVEVLGAASSTDSTAPTAAAGAPA"
     gene            4495..5253
                     /gene="purQ"
                     /locus_tag="VHM48_03660"
     CDS             4495..5253
                     /gene="purQ"
                     /locus_tag="VHM48_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2754531.1"
                     /translation="MSVRIGVVVFPGSNRDIDAVNALEVAGAEPVILWHDDASLAGVA
                     AVLLPGGFAYGDYLRAGVIARFSPVMRAVAGFADDGGLVLGVCNGFQVLTEAHLLPGA
                     LLRNRGLRFVSREVEIAAERLDTPFTHLLGERRALRMPVAHGEGAFYADEATLDELEA
                     NGQVLFRYVHADASPAGGEEDRANPNGSLRAIAGVMNAAGNVAGLMPHPETAVEAVLG
                     SDDGLGIIASFVASAAERERAGGPAVAGAAATGR"
     gene            5250..7598
                     /gene="purL"
                     /locus_tag="VHM48_03665"
     CDS             5250..7598
                     /gene="purL"
                     /locus_tag="VHM48_03665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX2754532.1"
                     /translation="MTMTVEAPPVHEEPLHRRLGVTDEELAAIRDRLGGRAPNDLELA
                     MFSVMWSEHCSYKSSRPLLKTLPTGSAADGVVAGPGENAGVIAIGDGLAVAFKIESHN
                     HPSAVEPTQGAATGVGGILRDIFTMGARPIAVLDALRFGDPADARTRHLVEGVVRGVG
                     GYGNCVGVPTVGGELVFDPSYAGNPLVNVMAIGLLAIDDLTLAAAPGVGNLVVLFGST
                     TGRDGIGGASVLASATFADDDPSKRPSVQVGDPFAEKLLIEASLELIRGGLVAGLQDL
                     GAGGITCATSETADRAGTGMHIELAAIPRREPDMEPFEVLISESQERMLAVVEPGRWG
                     EVRAVCERWGLPVAVIGRVTHDGDIAIAGDDGAEIARIPASALTSDAIVHQRVAAPPV
                     RRRAAPAPGAAATASDGLPERGMDPGAVLTALLGSANLASRRPVFEQYDSTVQANTVA
                     GPGHGAAIIRIKGTTKALVAATDANQAVGALDPWLGAALSVAEATRNVAITGARPLGV
                     TNCLNYGDPTRPEAFWQLTEGVRGLADACRALGLPVTGGNVSLYNESPTGAIAPTPEI
                     GVVGLLDDVALRVDPAWRAPGDRVLLVGEATPGLAGSAYAALAGLAAEDGPPSLDLER
                     ERRLQAFVREAAARGLLASAQDVSGGGLAVALAECALWGDGDHGLGARLRIGVASSPA
                     VDLFGESPSRVVASATPRHVPALVLLARQHGLPIEDLGEITARPDDGAPARLVVELHG
                     SGATGAAEDRGSRVADELTVGLDELRHAWEGGLSRALGWEQR"
     gene            7598..>8934
                     /gene="purF"
                     /locus_tag="VHM48_03670"
     CDS             7598..>8934
                     /gene="purF"
                     /locus_tag="VHM48_03670"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2754533.1"
                     /translation="MCGVFGVVMPPGGDQGAAALASLGLFALQHRGQESAGVAVSDGE
                     QLMLYKDLGMIASVLDERRLPSLRGGLAIAHCRYSTTGSTVWENAQPTFRLGPRRALA
                     IGHNGNLVNTRELLGQLTGGRGRLPASTDTELLTALLADEPAADTVDALVRVLPRVHG
                     AFSLVILDERRVIGVRDPFGFRPLVLGRLPAPDSAAAAAGPGLWGDEAGDDATSGWVL
                     SSETTGLDIVGAEYVRDVEPGEIVVLEAGRAPLSVRYAAAKPALCVFELIYFARPDSY
                     MEGRNLYEVRRRMGEELAGEHPAETDLVMPVPDTGAPAAAGYAEISGIPYREGLIRNR
                     YTGRTFIQPSQTMRHRGVTMKLNPLREVVRGKRLTVVDDSIVRGTTTKQIVALLRRAG
                     AAEVHVRISAPPIYHPCFYGIDTQVETELIAATHSVEEIRSFIGADSLGYLSIR"
BASE COUNT         1180 a         3315 c         3218 g         1221 t
ORIGIN      
        1 gcggatcgag gcgtggccat cctcggcgat cctgctcgac cgggcgacac cggtgtacga
       61 ggacttcgcg ggctgggagg agccgatcca cgaagtccgg gccctgtccg acctgcccga
      121 cgcggcgcgc cgctacgtga cggcgctcga ggagcatgcc ggggcgccga tcgtcctcgt
      181 ctcggtcggg ccggagcgga cccagacgat cgaacgcgcg tggcggccga tgcgccatcg
      241 cgtggttgcc ggcccatgag cgggacgcgg accggtgcgc ggtcacctgc gcatgccgtg
      301 agcgctgtga cgaatggtgc gtccactcgg ggctcccacg cccgggccgt cgggcattct
      361 gtgcacctgt cgagcgaact ggcggcagag gcaccccagg acggcgtccg acgggccctc
      421 gttcgtccca acgcgcaccg ggcgcgcagc cgatgaccca cctcgtccag ccgacccgga
      481 tcctggtcgt cggcggtggc gggcgggagc atgcgctggc ctggaagctg gccgcagagc
      541 cgggggtgaa cgaggtcgtc gtcgcgccgg ggtcggacgc gatcgcccgc gagccgcgtg
      601 ttcgggtcgt cccagggctg gatcctctcg acccggcggc ggttgtcgcc ctcgcccgca
      661 agcgcgcgat cgagttggtg gtcatcggcc cggaagcgcc gctcgcggcg ggcgtcgccg
      721 acgcgctcgg caccgccggc gtgccggtct tcgggccgac ggccgcggcg gcccggatcg
      781 agacatcgaa gaccttctgt cacgagatcg cagccgaagc cggcgtgccg atggcgaacg
      841 cggcgacgtt cagggcttcg gactccgcag aggccagggc gtgggcccgc gacgaggcag
      901 accgcgccgg atcgcggggc gtcgtcgtca aggccgacgg tctcgccgcc ggcaagggtg
      961 tcgtcgtctg cgataccgcc gccgaggccg gggcggcgat cgacgcgctc gtcgccggcg
     1021 ggaccgagcg gatcctcgtc gaggagcggc tcaccggccc cgaggcgacc gtcatcgcga
     1081 tctgcgatgg ccggtacgcc gtcgccctgc ccgctgcccg ggaccacaag cggctcggcg
     1141 acggcgacac gggcccgaac accggcggca tgggcgggta cagccccctg cccgacctcg
     1201 acgacgacgc cgtcgccgcc atcctcgcga ccgtccacga gccgattctg gcggccctcg
     1261 gccggcgcct gctggactac cgcgggttcc tctacgcagg cctgatgctg accgacgacg
     1321 gtccgcggct gctcgagtgc aacgcccgtc tcggcgaccc ggaggcgcag gtcatcctgc
     1381 cgcgtctggc tgggcccctc ggaccgctcc tcctgaccgc ggcgcgcggc cgcctcgagt
     1441 ccggcgcccg gatcccggtc ctgccgggag cggccgtcgg gatcgtcctc gccgcggccg
     1501 gctaccccgc cgccccgatc cgcggcgtgg ccatcgacgg gctggccgtc aacccggatg
     1561 ggctcgtgtt ccatgccggc acgcggcgcg acggcgacgg gtcgtggcgg gtcgacggcg
     1621 gccgggtgct gtgcgtcgtc gggcgcggtc cggacctggc ggcggcgcgc gtgatcgccg
     1681 aggttgccgc cgactcgatc agctggcccg gcgtccagcg ccgtcatgac atcggcgcgg
     1741 ccgcacggcc ttccgagccc gtcgcagccg cccgatgatc cgccgctaca cgctccccga
     1801 gatgggctcg atctggaccg agaaggcccg cttcgagcag atgctccggg tcgagcttgc
     1861 ggtcagccgg gcgcaggccg cccgcggcgt cgtcccggcc gacgccctcg ccaacatcga
     1921 ggcgcgagcg accgtcgacc ccgagcggat cgccgagatc gagcggacga ccgatcacga
     1981 cgtcatcgcc ttcgtgagcc aggtcgccga gactgtcggg cccgacggac gctatctcca
     2041 cctgggcctg accagcagcg atgtggtcga caccgcgctt gctctccagc tccgtgacgc
     2101 cggcgagctg ctcatccgcg actgcgatcg cctcctcgcg gtgctgatcg cccgggcccg
     2161 gagcgaggcc gacaccctga tgatgggccg gacccacagc gtccatgcgg agccgatcac
     2221 gttcgggctc aagcttgccg gctgggcgtt cgaggtcgat cgcggtcggc gccgtctcgc
     2281 gaccgccgtc gacgagatcg ggaccggcaa gatctcggga ccggtcggga cgtacagcca
     2341 tctggggccg gacgtcgagg cagaggtcct cgccgagctc gacctgcacg ccgacccgtc
     2401 gagcacccag atcgtccagc gcgaccgcca cgccgccctc ctcacgacca tcgcgatcct
     2461 cggcgggtcc ctggagcgct tcgcgaccga gatccgcaac ctccagcaca ccgagatcgg
     2521 cgaggtcatg gaggcgttca agcccggcca gaagggctcg agcgcgatgc ctcacaagcg
     2581 caacccgatc ctcgccgagc ggatcgccgg tctcgcccgt ctcctccgag gctacgccca
     2641 gaccgcgctc gagaaccagc cgctctggca cgagcgcgac atcagccata gcagcgccga
     2701 gcgggtgatc ctgcccgacg ccacgatcgt cctcgactac ctcctcgtga agacggcggg
     2761 cctcgtcgag ggcctcgtcg tccggcgcga gcggatgctc gagaacatcg agcgtggcca
     2821 cggactgcat gccagcagcc gcgtcctcgg caccctcgtc gagcgcggcg ggatgagccg
     2881 ggaggccgcc tacgccatcg tccagcgggc agcgctccgg gcagccgacg agcgctcgcc
     2941 cttgcgcgag ctccttgccc tcgatccgga ggtcgccaag cggatgagcc tggccgacct
     3001 cgacgcctgc ttcgacgacg ctgcccacct ccgccacgtc cctgccgtca tcgcccgact
     3061 cgacagcctg gaggccgaga tccatgccgc ccgctagccc gcttgcggac tcgttcctcc
     3121 ggtccggcaa ggtccgcgac ctgtacgagc tccccgacca ccggatggtc ctcgtcgcct
     3181 ccgaccggat cagcgcgttc gatgtcgtcc tcccctcgga gatcccggac aagggtcgcg
     3241 tcctcacggg catctcgcgt ttctggttcg cggagacgac gtcgatcgtc cgaaaccacc
     3301 tgcttgcgac cgacacgacg cagatcggcg acgccttcgc tgcgcgcggg atctcgagcg
     3361 aggcgatcgg cccgctggat cgcctgcggg gccgggtgat gatctgccgc cccgcgatcg
     3421 tgctgccgat cgaggccgtc gtgcgcggct accttgccgg ttccggctgg aaggagtacc
     3481 agcagaccgg aaccgtgtgc gggatcccgc tgccggccgg cctgcgcgag agcgaccgcc
     3541 tgcctgaacc gatcttcacg ccggcaacga aggccccgat cggcgagcac gacgagaaca
     3601 tcagcttcga cgggatgatc gaccatctcg cgggtgacgc gtgggtcctg ggcggcgccc
     3661 gcgagcacgc cccggcgctc gcggagcggg tacgagagat ttcgctcgcc ctctatcgat
     3721 tcggcgccgc ggttgccgag cgcaagggcg tcaccctcgc cgacacgaag ttcgagttcg
     3781 gcctcggcgg gtcgggcgac cctgacgacc tcgagcccgt gggcccaccg caggaacaga
     3841 ccggtgaact gcgcggcgaa ggctggctgg ccctgtccgc gaccggtagg ctcgagtcca
     3901 acctcatgct gatcgacgaa gtcctgaccc ccgactcctc gcgcttctgg gatgccgccg
     3961 attacgagcc cggccggtcc caggcgagct tcgacaagca gtacgtccgg gactggctcg
     4021 agacgcagcc ctgggacaag acggctccag gtccggagct gcccgccgac gtcgtgaccg
     4081 ggacgcgggc ccgctacgtc gaggcattcg agcggatcac cggggcgagc ttccagcgct
     4141 acctggacgc cgacgtgatc ggcgtggacg ggtcggacgg gcagaaggcg tgacggtgga
     4201 cacgaggttc gcggtcaacg tcagcccgaa gccgggcatc ctggacccgc agggtcgggc
     4261 cgtcgagggc agcctcgacc atctcgggat cgccggcgtc agcgcggtcc gggtcgggcg
     4321 gcgggtcgag ctcacggtca gcgcggccga cgacgcggcc gcccgagcgg tcgtcgaacg
     4381 cctggcggcc gagctgctct cgaaccccct gatcgaggcg tacgacgtcg aggtcctcgg
     4441 ggcggcctca tcgacggact cgaccgctcc gaccgccgcc gcgggggcgc ccgcgtgagc
     4501 gtccggatcg gggtcgtcgt cttcccgggc tcgaaccgcg acatcgacgc ggtcaacgcc
     4561 ctcgaggtcg cgggcgccga gccggtgatc ctgtggcacg acgacgcgtc gctcgccggc
     4621 gtcgccgcgg tcctgctgcc cggcggcttc gcctacggcg actacctgcg ggccggcgtg
     4681 atcgcccggt tcagcccggt catgcgggcg gtcgcgggct tcgccgacga cggcgggctc
     4741 gtcctcgggg tctgcaacgg cttccaggtc ctgaccgagg cccacctgct gccgggcgcg
     4801 ctccttcgca accgcgggct gcggttcgtg tcgcgcgagg tcgagatcgc cgcggagcgg
     4861 ctcgacacgc cgttcaccca cctgctcggc gagcggcgcg cgctgcggat gccggtcgcc
     4921 cacggcgaag gcgcgttcta cgccgatgag gcaaccctcg acgagctcga ggccaacggc
     4981 caggtgctct tccgctacgt ccacgccgac gcgtcgccgg cgggtggcga ggaggatcgg
     5041 gcgaacccca acggctcgct ccgggcgatt gcgggggtga tgaacgcagc cggcaatgtc
     5101 gcgggcctga tgccgcaccc ggagaccgcg gtcgaggcgg tcctcggctc ggacgacggg
     5161 ctcgggatca tcgcctcgtt cgtggcgagc gcggcggagc gggagcgggc cggcgggccg
     5221 gcggtggccg gcgcggcggc caccggccga tgacgatgac ggtcgaggcg ccgccggtgc
     5281 acgaggagcc gctccaccgc cggctcggag tgaccgacga ggagctcgcc gcgatccgcg
     5341 accggctcgg cgggcgggcg cccaacgacc tcgagctcgc gatgttcagc gtgatgtgga
     5401 gcgagcactg ctcgtacaag agctcgcggc cgctcctcaa gacgctgccg accggctccg
     5461 ccgcggacgg cgtggttgcc ggaccgggcg agaacgccgg cgtcatcgcg atcggcgacg
     5521 gtctggcggt cgcgttcaag atcgaatcgc acaaccaccc gagcgcggtc gagcccaccc
     5581 agggcgcagc gaccggtgtc ggcggcatcc tccgcgacat cttcacgatg ggggcccggc
     5641 cgatcgcggt cctcgacgcc ctccggttcg gtgacccggc ggatgcccgg acgcggcatc
     5701 tcgtcgaggg cgtcgtgaga ggtgtcggcg gctacggcaa ctgcgtcggc gtgccgacgg
     5761 tcggcggcga gctcgtgttc gacccgtcct acgccgggaa cccgctcgtc aacgtcatgg
     5821 ccatcggact cctcgcgatc gacgacctga ccctcgcggc cgcccccggc gtcggcaatc
     5881 tggtcgtcct cttcggttcg acgacggggc gcgacgggat cggcggggcg agcgtccttg
     5941 ccagtgcgac cttcgcggac gatgacccct cgaagcgacc gtctgtccag gtcggcgacc
     6001 cgttcgcgga gaagctcctg atcgaagcct cgctcgagct catccggggt gggctggtcg
     6061 ccgggctcca ggatctcggg gccggcggga tcacctgcgc gacctcggag acggccgacc
     6121 gggccgggac cgggatgcac atcgagctgg cggcgatccc gcgccgcgag ccggacatgg
     6181 agccgttcga ggtcctgatc agcgagtcgc aggagcggat gctcgcggtc gtcgagcccg
     6241 gtcgctgggg cgaggtcagg gccgtctgcg agcggtgggg cctgccggtt gcggtgatcg
     6301 gtcgggtgac tcacgacggc gatatcgcga tcgccggcga cgacggcgcg gagatcgccc
     6361 gaatcccggc ctccgccctc accagcgacg ccatcgttca ccagcgggtc gcggccccac
     6421 ccgtccggcg ccgcgccgcg cccgccccgg gtgcagcggc gaccgcgtcg gacggcctgc
     6481 ctgagcgcgg gatggacccc ggcgcggtgc tcaccgcgct gctcggctcg gccaacctcg
     6541 ccagccgccg cccggtcttc gagcagtacg actcgaccgt ccaggcgaac acggtcgccg
     6601 gtcccggcca cggcgcggcg atcatccgga tcaagggcac gaccaaggcg ctcgtcgccg
     6661 cgacggacgc gaaccaggcc gtgggtgccc tcgacccgtg gctgggcgcc gcgctgagcg
     6721 tcgccgaggc tacccgcaac gtggcgatca ccggcgcccg ccccctcggc gtcacgaact
     6781 gcctgaacta cggcgacccg acccgaccgg aggcgttctg gcagctgacc gaaggcgtcc
     6841 gtggcctcgc cgatgcctgt cgagcgctcg ggctgcccgt gaccggcggc aatgtctcgc
     6901 tctacaacga atcgccgacc ggggcgatcg cgccgacgcc cgagatcggc gtcgtcggtc
     6961 tcctcgacga cgtcgcgctg cgggtcgatc ccgcgtggcg tgcgccgggc gatcgggtcc
     7021 tcctcgtcgg cgaggcgacg cccgggctcg ccggcagcgc gtacgccgcg ttggcgggtc
     7081 tcgccgccga ggacgggccg ccgagcctcg atctcgagcg agagcggcgg ctccaggcgt
     7141 tcgtccggga ggccgccgcc cgcgggctcc tcgcctcggc ccaggacgtg tccggcggcg
     7201 gcctggccgt ggcgctggcc gagtgcgcgt tgtgggggga tggcgatcac ggtctcggcg
     7261 cccggctgcg gatcggcgtc gcgagctcgc ccgcggtcga cctgttcggc gagagcccgt
     7321 cgcgggtcgt cgcgtccgcc acgccgcgcc acgtcccggc gctcgtcctg ttggctcggc
     7381 agcacgggct gccgatcgag gacctcggag agatcaccgc gcgacccgac gacggtgcgc
     7441 ccgcccggct cgtcgtcgag ctccacggat cgggcgccac gggcgcggcc gaggatcgcg
     7501 gcagtcgcgt cgccgacgag ctgaccgtcg gcctcgacga gctccgccac gcctgggagg
     7561 gcggcctgtc gcgtgccctc ggctgggagc agcgctgatg tgcggcgtgt tcggcgtcgt
     7621 catgccaccc ggcggcgacc agggggcggc cgccctggcc tcgctcgggc tgttcgccct
     7681 tcagcaccgc ggccaggaat cggccggggt cgcggtcagc gacggcgagc agctgatgct
     7741 ctacaaggac ctcgggatga tcgcgtcggt cctcgatgaa cggcgcctgc cgtcgctccg
     7801 gggcgggctt gccatcgccc attgccgcta ctcgacgacc ggctcgacgg tctgggagaa
     7861 cgcccagccg acgttccggc tcggaccgcg gcgagccctc gcgatcggcc acaacgggaa
     7921 cctcgtcaac acccgcgagc tgcttggcca gctcactggc ggccgcggcc ggctccccgc
     7981 ctcgaccgac acggagctcc tgaccgccct cctcgccgac gagcccgccg cggacacggt
     8041 cgatgccctc gttcgcgtcc tgccccgcgt ccacggcgcg ttcagcctgg tcatcctcga
     8101 cgagcggcgg gtgatcggcg tccgggaccc gttcggcttc cggccgctcg tgctgggccg
     8161 cctgcccgcg cccgattcgg ccgccgcagc cgccggcccc gggctgtggg gcgacgaggc
     8221 cggcgacgac gcgacgtccg gctgggtcct ctccagcgag acgaccgggc tcgacatcgt
     8281 cggcgcggag tacgtccgcg acgtggagcc cggcgagatc gtcgtcctcg aagcgggccg
     8341 ggcgccgctg tccgtccgct atgccgccgc caagccggcc ctctgcgtct tcgagctcat
     8401 ctacttcgcc cggcccgact cctacatgga agggcgcaac ctgtacgaag tccggcgacg
     8461 gatgggcgag gagctcgccg gcgagcaccc ggccgagacc gacctcgtga tgccggtgcc
     8521 cgacactggt gcgccggccg ccgcgggtta cgcggagatc tccgggatcc cgtaccgcga
     8581 gggactgatc cgcaatcgct acaccggccg gacgttcatc cagccgagcc agacgatgcg
     8641 tcaccgcggc gtgacgatga agctcaaccc gctccgcgag gtcgtccggg gcaagcgcct
     8701 gacggtcgtc gacgactcga tcgtgcgcgg gacgacgacc aagcagatcg tcgcgctcct
     8761 acggcgggcc ggcgccgccg aggtccacgt ccggatcagc gccccgccga tctaccaccc
     8821 gtgcttctac gggatcgaca cccaggtcga gaccgagctg atcgcggcga cccattcggt
     8881 cgaggagatc cgctccttca tcggcgcgga ttcgctcggc tacctctcga tccg
//
LOCUS       DATAIW010000108         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_154130, whole genome shotgun sequence.
ACCESSION   DATAIW010000108 DATAIW010000000
VERSION     DATAIW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154130"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VHM48_03675"
     CDS             <1..460
                     /locus_tag="VHM48_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754534.1"
                     /translation="RARPAQTHPCARCGAPVGLDVGLCERCNPLGLKDSASSQVHGSV
                     LLAVGLAIAGLAIAAHFAVAGIGPFTARVTAMAPGASAGVLIATIEARNDGQATGSAT
                     CRLTDPLDPALIHSTIVYTPRIAPGETIAFVQEVSYGSPDRLLAVVCNGP"
     gene            457..1314
                     /locus_tag="VHM48_03680"
     CDS             457..1314
                     /locus_tag="VHM48_03680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2754535.1"
                     /translation="MTEVEDPTEPGRSTDHSPELRSRWADELAFAVEIAAAAGVVLMD
                     HYERLERIDHKSSRDVVTEADHLSEELVIAAIRADFPGDAILAEESGAHAASASQAVN
                     GRTWVIDPLDGTVNYANGLPVFCVSIGFVVDGRPVAGAVLDPTRGETFAATADGPATL
                     NGRSIRASTKDKLADGVLSLSLSGRSVSSRARAIRREVRVSRSMGSASLALAYVGNGR
                     FDAFVQETSMSLWDIAAAGLVAERAGAIVTSFGGGPWYEPSRSTRTIGLIAAPTAFHD
                     RLVRLANAR"
     gene            complement(1575..2456)
                     /locus_tag="VHM48_03685"
     CDS             complement(1575..2456)
                     /locus_tag="VHM48_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX2754536.1"
                     /translation="MKIHLVDATYELFRAHYAPRPPVRGRDGIVLSGVSGLCDQLLYL
                     LREEGATHVGCATDRVIESFRNDLFPGYKSSVGMDPELLAQFPIAEAAIEALGVVLWP
                     MVEFEADDAIGAAAARFGADPEVERILICTPDKDMAQCVVDDRIVLRDRRRGITYDRA
                     GVLEKWGVEPASIPDYLALVGDSSDGYPGLPGWGAKSTAAVLRRYGSIDAIPPKASEW
                     DVSGVSGLVRLAAVLVEQHEAAILYRTLARLRLDAPLAQRSTAELEWHGVPRAAWESF
                     CDRWGLARLRGRPHRWA"
     gene            complement(2467..3078)
                     /locus_tag="VHM48_03690"
     CDS             complement(2467..3078)
                     /locus_tag="VHM48_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2754537.1"
                     /translation="MQRYDGRCALVIVDLQNDFADPAGGLAVHGGANLVPIINGEIAT
                     ARAAAAMIVFTQDWHPESTPHFARDGGTWPVHCVGGTWGAELHPEVDAEAAASAPRVR
                     KGVNGEDGYSGFTMRDPTTGETTPTELEGLLRAGGIGRVVVCGLATDYCVKATALDAA
                     RLGFETAVLLDAMRPVELEAGDEQRAIDEMTAAGVLPWSTRMR"
     gene            complement(3218..3616)
                     /locus_tag="VHM48_03695"
     CDS             complement(3218..3616)
                     /locus_tag="VHM48_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754538.1"
                     /translation="MTYEERVVRDEDPAPVSDRGGSVVERRVTRTPSGGELAGRVVIL
                     LFGIVQAFIILRIVLLLLNAREANDLVSFILNTSQIFVAPFNGILNVDNLKSGGSVLD
                     IAAVLALVGWTVLEALILAAVGVFRRERTI"
BASE COUNT          577 a         1419 c         1436 g          585 t
ORIGIN      
        1 tcgagcccga ccggcccaga cgcatccgtg cgcccgctgc ggggcgccgg tcgggctcga
       61 tgtcggcctg tgcgagcgct gtaatccgct cgggttgaag gacagcgcca gctcgcaggt
      121 gcacggttcg gtgctgctcg cggtcggcct cgcgatcgcc gggctcgcca tcgccgccca
      181 tttcgcggtc gccgggatcg gcccgttcac ggcccgggtc accgcgatgg cgcccggcgc
      241 ctcggccggc gtgctgatcg cgaccatcga ggcccgcaac gacggccagg ccaccggcag
      301 cgcgacctgt cgcctgaccg atccgctcga tccggccctg atccattcga ccatcgtgta
      361 taccccgcgg atcgctccgg gcgagaccat cgccttcgtg caggaagtca gctacggcag
      421 cccggatcgg ctgctggcgg tcgtctgcaa cgggccgtga ccgaggtcga ggatccgacc
      481 gagccgggtc gctcgaccga ccactcgcct gagctgcgct cgcgctgggc cgatgagctg
      541 gcgtttgccg tcgagatcgc tgctgccgcc ggggtcgtcc tgatggacca ctacgagcgc
      601 ctggagcgga tcgatcacaa gagctcacgc gacgtcgtga ccgaggcgga ccacctgtcc
      661 gaggagctcg tcatcgccgc catccgggcg gacttccccg gcgacgcgat cctcgccgag
      721 gagtccggcg cccacgccgc ctcggcgtcg caggccgtca acggccggac gtgggtgatc
      781 gatccgctcg acgggaccgt caattacgcc aacgggctgc ccgtgttctg cgtctcgatc
      841 gggttcgtcg tcgacggccg gccggtggcg ggggcggtcc tcgacccgac ccgcggggag
      901 acgttcgccg ccacggccga cgggccggcc acgctcaacg gccggtcgat ccgggcgtcg
      961 acgaaggaca agctggcgga cggcgtgctt tccctgtccc tgtccgggcg gtcggtctcg
     1021 agccgcgccc gggcgatccg ccgggaggtc cgcgtctcgc gctcgatggg ctccgcgtcc
     1081 ctcgccctcg cctacgtcgg caacggccgg ttcgacgcct tcgtccagga gacgagcatg
     1141 tcgctctggg acatcgcggc ggcaggcctc gtggccgagc gagccggcgc gatcgtcacg
     1201 tccttcggcg gcgggccctg gtacgagcca tccaggtcga cgcggacgat cggcctgatc
     1261 gcggcaccga cggccttcca cgaccgcctc gtccgtctcg ccaacgctcg ctgacgcgac
     1321 cctggagcgg ggaaccattt cgtccaccgt cgcgggcgga gattcagtct cgactggagg
     1381 gactggtcaa tggagcggcg gatcggcgcg aggtcgccgc gtggggcgtt gtcatcgcct
     1441 gagccgatgg cgccgcgact ggacgaaccc cgtcgttcgg catggcatcg aggtcctggg
     1501 tggcgcgagc gccccgtcga cggatcgacg gaacctgacc ctgccggaag ccttcgtcga
     1561 ctgcagccgg actctcaggc ccagcgatgc ggccggccgc gcagccgggc gagcccccac
     1621 cgatcgcaga aggattccca cgccgcgcgc gggacgccgt gccactcgag ctcggccgtc
     1681 gaacgctggg ccaggggagc atcgagccgg agccgggcca gggtccggta caggatcgcc
     1741 gcctcgtgct gctcgacgag cacggcggcc agtcgaacga ggccgctgac accgctgacg
     1801 tcccactcgc tcgccttcgg cgggatcgcg tcgatcgagc cgtagcggcg gaggacggcc
     1861 gcggtcgact tcgcgcccca acccggcaac ccggggtagc cgtcggacga atcgccgacc
     1921 agggcgagat agtccgggat cgacgccggc tcgacgcccc atttctcgag gacaccggcc
     1981 cggtcgtagg tgattccgcg gcggcggtcg cgaagcacga tccggtcgtc aacgacgcac
     2041 tgggccatgt ccttgtcggg cgtacagatc aagatccgct cgacctcggg gtcggcgccg
     2101 aagcgggccg ccgcggcgcc gatcgcgtcg tccgcctcga actcgaccat cggccagagg
     2161 accaccccga gcgcctcgat cgcggcctcg gcgatcggga actgggcaag aagctcggga
     2221 tccatcccga ccgacgactt gtagccgggg aagaggtcgt tccggaacga ctcgatgacc
     2281 cgatccgtcg cgcatccgac gtgcgtggcg ccctcctcgc ggaggaggta gaggagctgg
     2341 tcgcacagcc cggacactcc ggacagcacg atcccgtcgc ggccgcggac cggcggtcga
     2401 ggtgcgtagt gggcgcggaa gagctcgtag gtcgcgtcaa cgagatggat cttcaccctg
     2461 acgccatcag cgcatccggg tcgaccaggg gaggaccccg gccgcggtca tctcgtcgat
     2521 ggcccgctgc tcgtctcccg cctcgagttc cacggggcgc atcgcgtcga ggaggacggc
     2581 ggtctcgaag ccgagccggg ccgcgtcgag ggcggttgcc ttgacgcagt agtcggtcgc
     2641 gagcccgcag acgacgactc gcccgatgcc gccggcccgg agcagtcctt ccagctcggt
     2701 gggggtcgtc tccccggtcg tcgggtcgcg catcgtgaag cccgaatagc cgtcctcgcc
     2761 gttgacgccc ttgcggaccc gcggggcgct cgctgccgcc tccgcgtcga cctccgggtg
     2821 gagctccgcc ccccaggtgc ccccgacgca gtggaccggc cacgtgccgc cgtcccgggc
     2881 gaagtggggg gtcgactccg ggtgccagtc ctgggtgaac acgatcatcg ccgcggccgc
     2941 ccgagccgtc gcgatctcgc cgttgatgat cgggacgagg ttggctccgc cgtggaccgc
     3001 cagccctccg gccggatcgg cgaagtcgtt ctggaggtcg acgatgacga gtgcgcaccg
     3061 accgtcgtaa cgctgcatcc gcctacgcta gcaaacgtcg acgacggcgc cacgtgggcg
     3121 ccgtcgtcgg ttgaggagga gcgggtgcga gtcggtccgc cggctgttgc caacccggcg
     3181 tcgccggatg ccgccggcga cgcgaggttt cgatgggtca gatggtccgc tcgcgacgga
     3241 agacgccgac tgccgcgagg atcagggcct cgaggacggt ccagccgaca agggcgagga
     3301 ccgcggcgat gtcgaggacg gaaccaccgg acttcaggtt gtcgacgttc aggatcccgt
     3361 tgaacggggc gacgaagatc tggctcgtgt tcaggatgaa cgacacgagg tcgttggctt
     3421 cgcgggcgtt caggagcagc aggacgatcc gcagaatgat gaacgcctgg acgatcccga
     3481 acagcaggat cacgactcga ccggcgagct cgccgccgct cggggtccgg gtcacccggc
     3541 gctcgacaac gctcccgccg cggtcggaga ccggggccgg gtcttcgtcg cggacgacgc
     3601 gttcttcgta ggtcatggcg tgtgcctccc atccgtagcc gccgaagcgg cgggacatca
     3661 gtgtcgtccc gcgcgctttg cgccacgaac agcagaatca cgccccggca ttgcagcgag
     3721 gtgacggttg tgcgggtccc gcaccgacac gtcccgccgg gcggcattgc gcgtgacgca
     3781 cacgtcgccg cgacggccgg atcgggaggc ccgaaatcgg tcgagatctt gcacggacgg
     3841 gaacgaaccg acgcgcgacg cgaccggctc gtgccgagtc gatcgcgttg ttgcgccgga
     3901 ggcaacgtcc ggcttcccgc gtgggctctc ggcgtcgacg tgtgcgcctg acgcaacgtc
     3961 cggcctcccg cgtggaacgg cgcccgtctc gaaacgggcg ccgtgtggct ttcggat
//
LOCUS       DATAIW010000109         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_94564, whole genome shotgun sequence.
ACCESSION   DATAIW010000109 DATAIW010000000
VERSION     DATAIW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94564"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1044
                     /locus_tag="VHM48_03700"
     CDS             343..1044
                     /locus_tag="VHM48_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754539.1"
                     /translation="MSKNPFSGLRLVVSAAAVVGMIAASAGPVAADTTPPGDTSYSQN
                     GSSAELDASSCVSNGDDTVTCADQQIYVFTGKMTDSLSGVTHTSQLCVSLSNYTYSEL
                     TGDYVGTPTFEQGCRVDLPTGTIKIDSKLRSASLATTHLTVQDQACEKFGCDPGSARD
                     IVVGASWTGFGPLMTSKSRGSSNDGTCRSNESFKGSTRAADVVGSLDGQALGGDQFGY
                     ISSGKYSFRSSCTEV"
     gene            complement(1103..3007)
                     /locus_tag="VHM48_03705"
     CDS             complement(1103..3007)
                     /locus_tag="VHM48_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754540.1"
                     /translation="MGMPAAKPRDFRGSFRRLIGRLRPEGPLLIGVVLLAIVSVTLTV
                     VGPKILGNAVNTIFEGAFSLKLGQLAPGATQEQVIQDLREKGQNQLADMVATMHLTPG
                     HGVDFGALAGVVALLIGLYIVSSIFAWMQGYIMAGVTQRTAYRLRTDVEVKLGRLPLR
                     YFDTHPRGDLLSRVTNDIDNISQSLQQSLTQLITSLLTIVGVLILMLTISPLLAAISL
                     LAVPASLVVTVLIIRRSQKLFVAQWASTGALNGQVEEMHTGHAIVKAFGRQRQAVATF
                     NEENERLYSASYRAQFISGIIQPAMNFISNLNYVAIAVIGGLRVAAGQMTLGDIVAFI
                     QYSRQFTFPIIQTASIVNVLQSAVASAERVFELLDETEEVAQAASPADLDRAAGAVEF
                     ADVSFRYEPDRPLIDDLDLVVEAGRTVAIVGQTGAGKTTLVNLLMRFYEIDSGRISID
                     GIDLRELTRDDLRRQFGMVLQDSWLFHGTIRENIVFGKLDATEAALHAAAEAAHVDHF
                     VRTLPDGYDTVIDDDATNLSAGEKQLLTIARAFLADPPILILDEATSSVDTRTEVLIQ
                     RAMARLMKGRTTFVIAHRLSTIRDAHTILVMERGAIVEQGTHDELLARGGTYADLYQS
                     QFAEPLAEAV"
BASE COUNT          525 a         1099 c         1164 g          508 t
ORIGIN      
        1 gggcggccgg caccggatcg ccggcaaccg cggtggggtc gccgtcgccg accgcgagcg
       61 cggccggggt ggccgtcgcc gaccgccagt gacagaaggc ctcgatcgcg atcgagcgca
      121 ccgaggtcct gttcgatgtc gccgcgaccc cgctcggctg ggtcgctgtc gggaccaggc
      181 tcaacctgcc gtcggcccag gcgacgttcg cgccgggccg acgggcggtc gagtgggtgt
      241 cggcgagtcg acacctgacc gattcgcgga aacaagtgcc gatccagcgc gttgacgggt
      301 tagcgccgcg gaactcttcg cggccatctg ggaggtatcc ccgtgagcaa gaacccgttc
      361 tccggccttc gactcgtggt cagcgcggcc gcggtcgtcg gcatgatcgc cgcgtcggct
      421 ggccccgtgg cggccgacac gacaccgccc ggcgacacca gctacagcca gaacggcagc
      481 tcggcggagc tcgacgcgag ttcgtgcgtg tcgaatggcg acgacacggt cacctgcgcc
      541 gaccagcaga tctacgtgtt caccgggaag atgaccgaca gcctctccgg cgtgacccac
      601 acgagccaac tctgcgtcag cctcagcaac tacacgtatt cggagctgac cggtgactac
      661 gtcggaacgc cgacgttcga gcagggctgc cgggtcgacc tcccgaccgg gacgatcaag
      721 atcgactcga agctccgctc ggcatccctc gcgacgacgc acctgaccgt ccaggaccag
      781 gcctgcgaga agttcgggtg cgacccgggt tcggcgcgcg acatcgtcgt cggcgcgagc
      841 tggaccggct tcgggcccct gatgacctcg aagtcacgcg gctcgtcgaa cgacgggacc
      901 tgccggtcga acgagtcgtt caagggctcc acgcgcgcgg ccgacgtcgt cggatcgctc
      961 gacggacagg ccctcggcgg cgaccagttc ggctacatct cctcgggcaa gtacagcttc
     1021 cgctcgagct gcaccgaggt ctgatcaccc cgccacagcc gcggcccccg tccgttgggc
     1081 gggggccgtt ccctgtccgt gctcagaccg cttcggcgag cggctcggcg aactggctct
     1141 gatacaagtc ggcgtaggtg ccgccgcgag cgagcagctc atcgtgggtg ccctgctcga
     1201 cgatcgcccc gcgctccatg accaggatcg tgtgggcatc gcggatcgtc gagagccgat
     1261 gggcgatgac gaacgtggtc cggcccttca tcaggcgggc catggctcgc tggatgagga
     1321 cctcggtccg ggtgtcgacc gaggacgtcg cctcgtcgag gatcaggatc ggcgggtcgg
     1381 ccaggaacgc ccgggcgatc gtgaggagct gcttctcgcc ggccgagaga ttcgtggcgt
     1441 cgtcgtcgat gaccgtgtcg tagccatcgg gcagggtccg cacgaaatgg tcgacatggg
     1501 ccgcctcggc ggcggcatgg agggcggcct cggtcgcgtc gagcttgcca aacacgatgt
     1561 tctcgcgaat cgtgccgtgg aacagccacg agtcctggag gaccatgccg aactggcggc
     1621 ggaggtcgtc ccgggtgagc tcgcggagat cgatgccatc gatgctgatc cggcccgagt
     1681 cgatctcgta gaagcgcatc agcaggttca cgagggtcgt cttgccggcc ccggtctggc
     1741 cgacgatcgc gaccgtccgg ccggcctcga cgaccaggtc gagatcgtcg atcagcggcc
     1801 ggtccggctc gtagcggaag gagacgtcgg cgaactcgac ggcgccggcg gctcgatcga
     1861 gatcggccgg gctggcggcc tgggcgacct cctcggtctc gtcgaggagc tcgaagaccc
     1921 gctcggccga cgcaaccgcg gactggagga cgttgacgat gctcgcggtc tggatgatcg
     1981 ggaaggtgaa ctggcgcgag tattggatga acgcgacgat gtcgccgagc gtcatctggc
     2041 cggcggccac ccgcaaaccg ccgatgaccg cgatcgcgac gtagttcagg ttcgagatga
     2101 agttcatcgc cggctggatg atcccggaga tgaactgggc ccggtagctc gccgagtaga
     2161 ggcgctcgtt ctcctcgttg aacgtcgcca ccgcctgtcg ctggcggccg aaggccttca
     2221 cgatcgcgtg ccccgtgtgc atctcctcga cctggccgtt gagggcaccg gtcgaggccc
     2281 actgggcgac gaacagcttc tgcgagcggc gaatgatcag caccgtcacc acgagcgagg
     2341 ccggtacggc gagcagcgag atggccgcga ggagcgggct gatcgtcagc atcaggatga
     2401 ggacgccgac gatcgtcagg agcgaggtga tcagctgggt caggctctgc tggagcgact
     2461 ggctgatgtt gtcgatgtcg ttggtcaccc gactcagcag gtcgccccgg ggatgggtgt
     2521 cgaagtagcg caacggcagg cggccgagct tcacctcgac gtcggtccgc agccggtagg
     2581 cagtccgttg ggtgacgccg gccatgatgt aaccctgcat ccaggcgaag atcgagctga
     2641 cgatgtacag cccgatcagg agcgccacga cgcctgcgag ggcgccgaaa tcgaccccgt
     2701 ggccgggcgt caggtgcatc gtcgcgacca tgtcggccag ctggttctgg cctttctccc
     2761 tgaggtcctg gatcacctgc tcctgggtgg cgcccggagc cagctgaccg agcttgaggc
     2821 tgaacgcccc ctcgaagatc gtgttcaccg cgttgccgag gatcttcggc ccgacaacgg
     2881 tcaaggtgac gctgacgatc gccagcagga ccaccccgat gaggagcggg ccctcgggcc
     2941 ggaggcgacc gatgagccgc cggaacgaac cccggaagtc gcgcggcttg gcggcgggca
     3001 tgcccatccc catgcccatc atcccgccgc cgggacggcc gccgcccggg ccgcggggcg
     3061 gggcaccgcc gggagggccg cccgccggag gtccgcctga gggcgcgccg ggccgaccgc
     3121 tcatgccacg gcctccttct cggacatctg ggagtacacg atctcgcgat aggtctcgtt
     3181 gtcggccagc agctcgcggt gcgtgccgat gccgacgatc cggccgccgt ccatcacgat
     3241 gatccggtcc gcctgcatga tcgtgccgac ccgctgggcg acgatgatga tcgtgg
//
LOCUS       DATAIW010000110         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_64879, whole genome shotgun sequence.
ACCESSION   DATAIW010000110 DATAIW010000000
VERSION     DATAIW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64879"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..1870)
                     /locus_tag="VHM48_03710"
     CDS             complement(428..1870)
                     /locus_tag="VHM48_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2754541.1"
                     /translation="MADHRLSATQLVPRPIEEVFDFFSRPENLGRITPASMGFEFLTD
                     DRDMRDGLEVRYRIRPLLGIPMTWTTRITDYEPPTRFRDLQLRGPYTRWEHTHTFEAV
                     PGGTLVRDEIVYAVPLAPLGDLANKLVVRGELQRIFRHRAETIRTVFAAAAEPTGMTV
                     AVAGGTGFVGGAIATELHRRGHVVRVLSHRGETARGGLADAIDLRAADVQTGEGLVEA
                     LRGADALAIALAFKNSPIEAPRTHQTFMEVDAAGTERLVAAARAAGVKRIVYLSGAGA
                     APDAKRHWFRAKWRAEEAVRGSGLIWTIIRPTWIFGPRDVSLNRFVGFARRLFSVPMT
                     NTGSQLLAPVFIDDAANLAADSLVTDAAVNQVFELGGPETLKMREIIGTALRVAGLRR
                     PIVPGPTPLIKLLAIPLSWLPTPILTPDAVDFINQPAIVDLAPLLERMPRRLTPLEEG
                     LRTYLEPPSGDATLDFDRPVAPASSAPAHG"
     gene            2019..>4283
                     /locus_tag="VHM48_03715"
     CDS             2019..>4283
                     /locus_tag="VHM48_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2754542.1"
                     /translation="MPPNGQQPVDATTGSELDALRARVAALEAREAEHERSERVQGSL
                     YRIAEAASAATDLSEFYRTIHAIVGELMDAENLYVALYDAERALMNYPYFVDALDKDV
                     PDPNAWEPFGEGQARGITAYALRQGRPLRVDARLFHELESRGDVERLGIVTEEGTWLG
                     VPLSSEGRTLGLLVVQSYTAEHRYTDADLDLLAFVGQHIGSALTRVRAMDETRQRNAE
                     LALVNEIGQALAEQLDFGAIIELVGDRVRAIFDTRSLFIALHDPATDMLSFPYDIDEG
                     ERFDRGLLKLGPGVTSTVMRTGRSLRIGTLEEQIAAGAIQIGGSDSLSWLGAPIPAGN
                     RVIGVVGLESLRPHAFSEADERLLVTLATSMGVALENARLFDETKRLLAETDARAAEL
                     AVINQIGQALASQLDFEAIIQLVGERIRAMFTARTIYIGILDSTTNLISFPYDLMNGK
                     PIHTEPFALGPGLTSRVITTAAPLLIRSFEEAKRLGTIEDGLDAESWLGVPILTGAHV
                     LGVIAVEAEERGAYDESDLRLLSTLAASMGVALENARLFDETKRLLSETNERAAELAL
                     INDVQHGLAERLDMQAMYDLVGDRIQAIFDTQVVDIGVIDRAEGLIHFPYAIERGVRF
                     PDEPTPIRSFRKQVIETGRPMLLGKKELTAAMESGESMVIQGEPAQSMLIAPLSTGSA
                     VSGVISLQNLDRDGAFSKSDVELLTTLAASLSVALENVRLFDETKRLLSETNERAAEL
                     ALINDVQHGLAQKLD"
BASE COUNT          691 a         1500 c         1459 g          633 t
ORIGIN      
        1 gcgggcgatg tacgaggcga tcggcgagct ggagcgaagc gaggcggcgg tcgcgatctt
       61 cggccaggac gtcgtcgacc actacctcaa cgcggcccgg gtcgaacagc agacctacga
      121 cgcgatggtc cacaactggg agcgcgagcg gtacctcgag cggagttagg tctcgtcgcg
      181 gtctcgacaa ggcagcgtac gctcgcgggc ggtccgcggt cgggtgtccg ctccccgaat
      241 ctgtcaagtg aatccttggc ggtcggtagt cggccggaat ctgtctaccg ctcgcccggc
      301 gatcagatgt caggacatgg cacccgatcc acgctcagcc gtgccgagcc gttggaatcg
      361 catccggctt gcgaccgggt gagattcgtt tgacggaaac cgatgctggg cgacccgccc
      421 ccgagcgtca gccgtgggcg ggagccgagc tggccggagc gacgggccga tcgaaatcga
      481 gggtcgcgtc gccgctgggc ggttcgaggt aggtccggag accttcctcg agtggggtca
      541 ggcggcgtgg catgcgctcg agcagcggcg cgagatcgac gattgccggc tggttgatga
      601 agtcgaccgc atcgggggtc aggatcgggg tgggcagcca cgacagcggg atcgcgagca
      661 gcttgatcag gggcgtcggc ccgggcacga tcggccggcg cagcccggcc acccggagtg
      721 cggtcccgat gatctcccgc atcttcagcg tctcgggtcc gccgagctcg aagacctggt
      781 tcacggcggc gtcggtcacg agggaatcgg ccgcgaggtt cgccgcgtcg tcgatgaaga
      841 ccggggcaag gagctgcgat ccggtgttcg tcatcggcac gctgaacagc cgccgggcga
      901 aacccacgaa ccggttcagc gacacgtcgc gcggcccgaa gatccacgtg gggcggatga
      961 tcgtccagat cagtccggag ccgcggacgg cctcctcggc ccgccacttc gcccggaacc
     1021 agtggcgctt cgcgtccggc gcggccccgg cacccgacag gtagacgatg cgcttgacgc
     1081 cggccgcgcg agcggcggca accagccgct cggtgcccgc cgcatcgacc tccatgaacg
     1141 tctggtgcgt tcgaggcgcc tcgatcgggg agttcttgaa cgccagcgcg atcgcgagcg
     1201 catcggcgcc gcgcagtgct tcgaccagtc cttcgcccgt ctggacgtcg gccgcgcgaa
     1261 ggtcgatcgc atcggccagg ccaccacggg cagtttcgcc gcgatgggac agcacgcgaa
     1321 cgacgtggcc gcgccgatgc agctcggtcg cgatcgcccc gccgacgaag ccggtcccgc
     1381 cggcgacggc cacggtcatc ccggtcggct cggccgctgc cgcgaatacc gtccggatcg
     1441 tctccgcccg gtgccggaag atccgctgca gctcgccgcg gaccaccagc ttgttggcaa
     1501 ggtcaccgag cggcgcgagc gggacggcat agacgatctc gtcgcggacg agcgtgccgc
     1561 cggggaccgc ctcgaacgtg tgggtgtgct cccagcgggt gtacggaccc cgcagctgga
     1621 ggtcgcggaa gcgcgtcggc ggctcgtagt cggtgatccg ggtcgtccag gtcatcggga
     1681 tgccgagcaa cggccggatc cggtagcgga cctcgagtcc gtcgcgcatg tcgcgatcgt
     1741 cggtcaggaa ctcgaagccc atcgacgccg gcgtgatccg gccgaggttc tccggccgcg
     1801 agaagaagtc gaacacctcc tcgatcggcc ggggcaccag ctgcgtcgca gacagtcgat
     1861 ggtccgccat cggccgagta tatcgtgata cctcgcaata gcaagtttgt tgcctacccg
     1921 tcggcccggg atcggcccga accgctcgcc tcgcccgcgc tatcctcgcg accacagcgc
     1981 gtcaggacgg tcgggcggcg cgcggagatg ggagcagcgt gccgccgaac ggacagcagc
     2041 cagtcgacgc gacgaccgga agcgagctgg acgcgctccg cgcgcgggtc gccgccctcg
     2101 aggcgcggga ggccgagcac gagcgctcgg agcgcgtcca gggctcgctc taccggatcg
     2161 cggaggcggc gagcgccgcg acggacctgt ccgagttcta ccggacgatt cacgccatcg
     2221 tcggcgagct gatggacgcc gagaacctat acgtcgcgct ctacgacgcc gagcgcgcgc
     2281 tgatgaacta cccgtacttc gtcgacgcgc tcgacaagga cgtcccggat ccgaatgcct
     2341 gggagccgtt cggcgaaggc caggcccgcg gcatcaccgc ctatgccctc cgccagggtc
     2401 gaccgctgcg cgtggacgcg cgtctcttcc acgagctcga gtcgcgtggc gacgtagaga
     2461 ggctcggcat cgtgaccgaa gagggaacct ggctcggcgt gcccctctcg tccgagggcc
     2521 ggacgctcgg gctgctcgtc gtgcagtcgt acacggcgga gcatcgttac accgacgctg
     2581 atctcgacct cctcgccttc gtcggccagc acatcggctc cgccctgacc cgtgtccggg
     2641 cgatggacga gacgcgccag cggaacgccg agctggccct cgtcaacgag attggccagg
     2701 ccctggcgga gcagctcgac ttcggcgcga tcatcgaact cgtcggcgac cgcgtccggg
     2761 caatcttcga cacccgctcg ttgttcatcg ccctccacga cccggcgacc gacatgctct
     2821 cgtttcccta cgacatcgac gagggcgagc ggttcgaccg cggcctgctc aagctcggcc
     2881 cgggcgtgac ctcgaccgtc atgcggaccg gccggtcgct gcggatcgga accctcgagg
     2941 agcagatcgc cgccggcgcg atccagatcg gcggatcgga ctcgctttcg tggctcggcg
     3001 ccccgattcc ggccggcaat cgcgtcatcg gagtcgtcgg tctcgagagc ctccgtcccc
     3061 acgcgttcag cgaggccgac gaacgcctcc tcgtgacgct cgcgaccagc atgggcgtcg
     3121 cgctcgagaa cgcccgcctg ttcgacgaga cgaagcgcct gctcgcggag acggacgccc
     3181 gcgccgcgga gctcgcggtc atcaaccaga tcggccaggc gctcgccagc cagctcgact
     3241 tcgaggcgat catccagctc gtcggcgagc ggatccgggc gatgttcacg gcccggacga
     3301 tctacatcgg gatcctggat tcgacaacga atctcatctc gttcccgtac gacctcatga
     3361 acggcaagcc gatccacacg gagccgttcg ccctcggccc cgggctcacc tcgcgcgtca
     3421 tcacgaccgc cgcaccgctc ctcattcgca gcttcgagga ggccaagcgc cttggcacga
     3481 tcgaggacgg gctcgatgcc gagtcgtggc tcggcgtccc gatcctcacc ggcgcgcacg
     3541 tcctgggggt catcgcggtg gaggcggaag aacggggtgc gtacgacgaa tccgatctgc
     3601 ggctgctctc caccctcgcc gcgagcatgg gcgtcgccct cgagaacgcc cgcctgttcg
     3661 acgagaccaa gcgcctcctg tccgagacga acgagcgcgc cgccgagctg gccctgatca
     3721 acgacgtcca gcacggcctg gccgaacgac tcgacatgca ggcgatgtac gacctggtcg
     3781 gcgaccggat ccaggcgatc ttcgacaccc aggtcgtcga catcggagtg atcgaccgcg
     3841 ccgagggctt gatccatttc ccctacgcga tcgagcgcgg cgtccgcttc cccgatgagc
     3901 cgacgcccat ccggagcttc cgcaagcagg tcatcgagac cggccggccc atgctccttg
     3961 gcaagaagga gctcaccgcc gcgatggagt ccggcgagtc gatggtcatc cagggcgagc
     4021 cggcgcagtc gatgctcatc gcgccgctgt caacgggctc ggcggtcagc ggcgtgatca
     4081 gcctccagaa tctcgaccgt gacggcgcct tctcgaagtc cgacgtcgag ttgctgacca
     4141 ccctcgcggc cagcctcagc gtcgccctcg aaaacgtgcg cctgttcgac gagacgaagc
     4201 gcctcctgtc cgagacgaac gaacgcgccg ccgagctggc cctgatcaac gacgtccagc
     4261 acggtctggc ccagaagctc gac
//
LOCUS       DATAIW010000111         5911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_35205, whole genome shotgun sequence.
ACCESSION   DATAIW010000111 DATAIW010000000
VERSION     DATAIW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35205"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VHM48_03720"
     CDS             complement(<1..478)
                     /locus_tag="VHM48_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754543.1"
                     /translation="MTVDRADGPFRPGLDRDPDRPRSVCRFLAVEATDGTLEPAVGGV
                     DAANRCVAIGEPVPQSGRQQELVCLAAAHVNCPRYLRGVLLAATPPPQPTRQPVSRAV
                     IGASLVLAASLAASFGFLAVRGGFNLPTVSAPPALVAVVGSPSPQPSPNPTVEATPT"
     gene            674..898
                     /locus_tag="VHM48_03725"
     CDS             674..898
                     /locus_tag="VHM48_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754544.1"
                     /translation="MVTSEPDPYRLAPHRIKRCTFRRLIQIESGTERLYDVECLLPDR
                     RLPIPLGNLDAAMPICNACVAPHTFRPDED"
     gene            complement(1020..1384)
                     /gene="rnpB"
                     /locus_tag="VHM48_03730"
     ncRNA           complement(1020..1384)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHM48_03730"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1468..1941)
                     /locus_tag="VHM48_03735"
     CDS             complement(1468..1941)
                     /locus_tag="VHM48_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX2754545.1"
                     /translation="MRRLRAARLASVRVVAVFVLIVSGACAPAPSASLIRVSHPASLV
                     GTAWRLVSIDGHKPPASPDVTIAFGPSDISGDGPCNSFGGTYAYDPTIGALQFGPLVS
                     TKRACVDPVLNDAEAAYFGALHGVVGASDDPNGRLVLAGAGPELVFEVGPQLVPA"
     gene            complement(1938..2429)
                     /locus_tag="VHM48_03740"
     CDS             complement(1938..2429)
                     /locus_tag="VHM48_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX2754546.1"
                     /translation="MPRSLAPVRLVALIVLLAIVGAISGCGSDGPPTGSAPGSPPDGP
                     LPALAGTAWIVISVNGRSPIAGAVPTIVFDAARASGFGGCNQFGGSYQFDATSGRFVV
                     HEIAATAMGCLQAGVGDFETAFMTALGGSTQAGLDPTGQLILSGPAARIVLVTLEHPG
                     AGG"
     gene            complement(2455..2988)
                     /locus_tag="VHM48_03745"
     CDS             complement(2455..2988)
                     /locus_tag="VHM48_03745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX2754547.1"
                     /translation="MFPDLPATGEESGSRGGADRPRPRGYLIRTDGAARGNPGPASAG
                     AALIDLARADARDPRARPDASISEFLGVQTNNVAEYTAVVRALALAAELGAREVVMLL
                     DSKLIVEQLAGRWRVKDSKLIPLWETSRRTLRGFERWSATHVPRAQNKTADALCNEAI
                     DRVAAGGPAVVVVRPGT"
     gene            complement(2997..3701)
                     /locus_tag="VHM48_03750"
     CDS             complement(2997..3701)
                     /locus_tag="VHM48_03750"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX2754548.1"
                     /translation="MQVDLRILRYDPERDEKPHWEAYTVESDPKADRVLDLLHRVKYE
                     QDGTLTFRRSCGHGVCGSDAMLVNGRNRLACKVRVEQLGKRRITIAPLPGLPVVKDLV
                     VDMEGFFAKIRSVQPFLIEAGPAPTRERIQSPEDRARYDDTTKCIQCAACTSSCPSFW
                     AQPSYVGPAAIVNAHRFIFDSRDAGGDERLEILADKDGVWRCRTIFNCTDACPRGIHI
                     TQAILEVSSAIVERQT"
     gene            complement(3722..5473)
                     /gene="sdhA"
                     /locus_tag="VHM48_03755"
     CDS             complement(3722..5473)
                     /gene="sdhA"
                     /locus_tag="VHM48_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX2754549.1"
                     /translation="MRINHDALIVGAGGAGLWAALELAKAGVDAAVITKLYPTRSHTG
                     AAQGGVCAALGNQEEDHWEWHMFDTIKGGDYLVDQDAAEILAREAIETVIELEHMGLP
                     FNRTPDGRIDQRRFGGHTRNYGEAAVRRACFAADRTGHMILQTLYQQCIKYGVKFYDE
                     YHVVDLLSEGGDLGDGGRAAGVVAYRIADGEIQTFRSKAVLLATGGFGRMFRITSNAW
                     SLTGDGVALAYRHGVPMQDMEFYQFHPTGIYGIGILLSEAARGEGGILLNGEGEAFMA
                     RYAPSMKDLAPRDVVSRSMYLEIRDGRGVGGKDYLYLDVRHLGAKVIEEKLPDITDFA
                     RVYLGVEPLTEPIPIQPTAHYAMGGIPTDVLTRVTRDAAKTVVPGLYAAGECACVSVH
                     GANRLGTNSLVDLLVFGRRAGRQMALDVKGVGMPELARDVEDPVRGEMAALKANIKGE
                     NAARIRKELADVMMDNVGVYRDEALLSAALPKVRELKQRYAKVGIADRGSVFNTDLLE
                     AREIGYLLDCAETTVVAALNRKESRGAHAREDYPTRDDVNFLTHSLATRGPGGGNPQL
                     TYKPVTITTFQPKPRIY"
     gene            complement(5476..>5911)
                     /locus_tag="VHM48_03760"
     CDS             complement(5476..>5911)
                     /locus_tag="VHM48_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754550.1"
                     /translation="SSNRSGAAEMSANRPLGRSGSRARPSGGGFELAVWYLMRLTGLG
                     LFVLAISHFLITHVLYDPVQQDSSFISERWANLSVRTADWLMLAFVVFHAFMGLRIIV
                     GDYTRGGLRTVLTMGLYLLAIVIFAMGSIALLTVPSILPRTT"
BASE COUNT          885 a         2034 c         2109 g          883 t
ORIGIN      
        1 gcgtcggggt cgcctcgacc gtcgggttgg gcgacggctg cggcgacggg ctgccgacga
       61 cggcgaccag cgccggcggt gcgctgacag tgggcaggtt gaagccgccg cggaccgcaa
      121 ggaacccgaa ggatgcggcg agggacgcgg cgagaacgag cgaagcgccg atcacggcac
      181 gcgagaccgg ctgccgcgtg ggctgtggcg gcggggttgc ggcgagaagg acgccgcgca
      241 ggtagcgcgg acagttgacg tgggccgcgg ccaggcacac gagctcctgc tggcgaccgg
      301 actggggcac gggctccccg atcgcgacgc agcggttcgc cgcgtcgacg ccgccgaccg
      361 ccggctcgag cgtcccgtcc gtcgcctcga cggccagaaa ccggcagacc gaacgcggtc
      421 gatcggggtc gcgatcaagg cctgggcgga acggcccatc ggcccgatcg acggtcaacg
      481 cgtcgttcgt cgtcgttctc tcgccctcct ccacgcgccc tccgcgctca gaccgccatg
      541 gacggcgcgg agtctagtac gtttagcgtg ttgccgtgac gctcaccgcg gtgctacatt
      601 ccgccgccag atcggcatat cgcgtccgcc gatccctccg aggcacctcg cccagatggc
      661 catgccgctt tcgatggtca cgtcagagcc ggatccgtat cgcctcgcgc cccatcggat
      721 caagcgctgc acgttccgtc ggctcatcca gatcgagagc ggcaccgaac gcctctatga
      781 cgtcgagtgc ctcctccccg accgccgcct gccgattccg ctcggcaacc tcgacgcggc
      841 gatgccgatc tgtaacgcct gcgtcgcgcc ccacaccttc cgccccgacg aggactgaaa
      901 cctcgggccg atggcgtcgc ggagctccac ctcgggcgga tggcttcgcg gagctcagcc
      961 tccgacctcg gtgagcgagt gacgggcgac agctcagggt gttggttcac cggaatgtgt
     1021 cagcgaggca gtctgtacgc cgagttctgt cacggcggct tgcgccgccg cggatggcca
     1081 tccatctgag gccgccggtt acccgacggc tcttgcggcc gacccgaggg ctgggcagcg
     1141 cacctctccc ccgacgggtt gccccgcacg ggttgcgccc tcctatttgg ccttgctccg
     1201 ggtggagttt gccgcgtttc actccgtcga cggcttgcgc cgccaacggc atcgtcactg
     1261 tggcactggt cctcgtctca cgacggacgg gcgttacccg ccaccctgcg ctgcggagct
     1321 cggacgttcc tcacgccgtg gacggggttg cccccgagcg gcgcgcggcc atccgaccac
     1381 ctcgctgacg aggtcattct acggccggca cgattctcct cggctgcacg tccccgacga
     1441 cctgcagcgc gaccgacggt gacagcgtca cgccgggacc agctgcggcc cgacctcgaa
     1501 gacgagctcc ggacccgcac cggcgaggac cagccgaccg ttcgggtcgt cgctcgctcc
     1561 cacgacacca tggagggcgc cgaagtacgc ggcctcggcg tcgttcagga ccggatcgac
     1621 gcaggcacgc ttggtggaga cgagagggcc gaactgcagg gcaccgatcg tcgggtcgta
     1681 ggcataggtc ccgccgaagc tgttgcacgg gccgtcgccg gagatgtcgg agggcccgaa
     1741 cgcgatcgtc acatcggggc tggccggcgg cttgtggcca tcgatcgaga cgagccgcca
     1801 cgccgtcccg acgagcgagg ccgggtggct gacgcggatc aatgaagcgg acggcgctgg
     1861 cgcgcaggcg ccgctgacga tcaagacgaa gaccgcgacg acccggaccg acgccaaccg
     1921 tgcagcccgc aggcgcctca cccgcccgca cccgggtgct cgagggtgac caggacgatc
     1981 cgcgcggcgg gtccgctcag gatgagctgg ccggtcgggt cgaggccggc ctgggtcgac
     2041 cctccaaggg ccgtcatgaa tgccgtctcg aagtcgccca cgccggcctg caggcagccc
     2101 atcgccgtcg cagcgatttc gtgaacgacg aaccgtccgg acgtcgcgtc gaactgatag
     2161 ctgccaccga actggttgca cccgccgaac ccggaggccc tcgccgcgtc gaacacgatc
     2221 gtcggcacgg ctcccgcgat cggcgaccgc ccgttgaccg agatgacgat ccaggcggtc
     2281 ccggcgagag caggaagggg cccatcgggc ggcgagcccg gcgctgagcc ggtgggcgga
     2341 ccgtcgctac cgcatccact gatggcgccc acgatcgcga gaagcacgat gagcgcgacg
     2401 agccggacgg gggcgaggga gcggggcatg ggcggaggac gacgcgaccc gccgtcaggt
     2461 tccggggcga acgacgacga ccgccggccc gcccgcggcc acccgatcga tggcctcgtt
     2521 gcagagcgcg tcggcggtct tgttctgggc tcgcgggacg tgggtcgcgg accagcgctc
     2581 gaagccgcgg agggtccgtc gcgaggtctc ccagagcggg atcagcttgc tgtccttgac
     2641 ccgccagcgg ccggccagct gctccacgat cagcttcgag tcgaggagca tcacgacctc
     2701 gcgggcaccc agctcggcag ccagcgccag cgcccggacg accgcggtgt actccgcgac
     2761 gttgttggtc tggacgccga ggaactccga gatcgaggca tcgggtcgag cccgcgggtc
     2821 gcgggcgtcg gcccgggcaa ggtcgatgag cgcggcaccg gccgatgccg gacctgggtt
     2881 tccgcgggcg gcaccgtcgg tccggatgag gtagccacgc ggccggggcc ggtcagcgcc
     2941 accacgcgag ccgctctcct cccccgtcgc gggcaggtcc ggaaacaacg tgtcgttcag
     3001 gtctggcgct ccacgatcgc cgagctgacc tcgaggatcg cctgggtgat gtggatgccg
     3061 cggggacacg cgtcggtgca gttgaagatc gtgcgacagc gccacacgcc gtccttgtcg
     3121 gccaggatct cgaggcgctc gtcaccgccg gcatcgcgcg agtcgaagat gaagcggtgg
     3181 gcgttgacga tcgcggccgg accgacgtag ctcggctggg cccagaacga cgggcaggac
     3241 gacgtgcagg cggcgcactg gatgcatttc gtcgtgtcgt cgtagcgggc ccggtcctcg
     3301 gggctctgga tccgctcgcg ggtcggggcc ggccccgcct cgatcaggaa cggctggacg
     3361 ctccggatct tcgcgaagaa gccctccatg tcgacgacga ggtccttgac gaccggcagt
     3421 ccgggcagcg gcgcgatcgt gatccggcgc ttgccgagct gctcgacccg gaccttgcag
     3481 gcgagtcggt tgcggccgtt gacgagcatc gcgtccgagc cgcagacccc gtggccgcag
     3541 gaccggcgga aggtcagggt cccgtcctgc tcgtacttga ctcgatggag cagatcgagg
     3601 acgcgatcgg ccttcgggtc ggactcgacc gtgtaggcct cccagtgcgg cttctcgtcg
     3661 cgctcggggt cgtagcggag gatgcggagg tcgacctgca tgctggttgg cgccccttgg
     3721 ctcagtagat ccgcggcttg ggctggaacg tcgtgatcgt gaccggcttg taggtcagtt
     3781 gcggattccc gccgccgggg ccgcgggtcg cgaggctgtg ggtcaggaag ttgacgtcgt
     3841 cccgggtcgg gtagtcctcg cgagcgtggg cgccgcggct ctccttgcga ttgagggcgg
     3901 cgacgaccgt cgtctcggcg cagtcgagga gatagccgat ctcgcgcgcc tcgagaaggt
     3961 ccgtgttgaa gaccgagccg cggtcagcga taccgacctt tgcgtagcgc tgcttcagct
     4021 cgcggacctt cggcagggcg gccgacagga gggcctcgtc gcgatagacc ccgacgttgt
     4081 ccatcatcac gtcggcgagc tccttgcgga tccgggccgc gttctcgccc ttgatgttcg
     4141 ccttgagggc cgccatctcg ccgcggaccg ggtcctccac gtcgcgggcc agctccggca
     4201 tcccgacgcc cttgacgtcg agcgccatct ggcgaccggc ccgccggccg aacacgagga
     4261 ggtcgacgag cgagttcgtg ccgagccggt tcgcgccgtg gacgctgacg caggcgcact
     4321 cgcccgccgc gtacagcccg ggcacgaccg tcttcgcggc gtcgcgggtg accctggtga
     4381 ggacgtcggt cgggatcccg cccatcgcgt agtgggccgt cggctggatc gggatcggct
     4441 cggtcaacgg ctcgacgccg agatagaccc gggcgaagtc ggtgatgtcg ggcagcttct
     4501 cctcgatcac cttggccccg agatggcgga cgtcgaggta gagatagtcc ttgccgccca
     4561 cgccccgccc gtcgcggatc tcgaggtaca tcgagcggga gacgacgtcg cgcggggcga
     4621 ggtccttcat cgacggcgcg tagcgggcca tgaatgcctc gccctcaccg ttgagcagga
     4681 tcccaccctc gccgcgggcc gcctcggaca gcaggatccc gatcccgtag atcccggtcg
     4741 ggtggaactg gtagaactcc atgtcctgca tcgggacgcc gtggcgatag gccagggcca
     4801 ccccgtcgcc ggtcagcgac caggcgttcg aggtgatccg gaacatccgt ccgaacccgc
     4861 ccgtggcgag caggacggct ttcgagcgga acgtctggat ctcgccgtcc gcgatccggt
     4921 aggccacgac ccccgctgcc cgtccgccat cgccgagatc gccaccctcg ctcagcaggt
     4981 cgacgacgtg gtactcgtcg tagaacttga cgccgtactt gatgcattgc tgatagagcg
     5041 tctgcaggat catgtggccg gtccggtcgg cggcgaagca cgcccggcgc acggcggcct
     5101 cgccgtagtt ccgggtgtgg ccgccgaacc gccgctggtc gatccggccg tcgggcgtcc
     5161 ggttgaacgg cagtcccatg tgctcgagct cgatcacggt ctcgatcgcc tcgcgggcca
     5221 ggatctccgc cgcgtcctgg tcgacgaggt agtcgccgcc cttgatcgtg tcgaacatgt
     5281 gccactccca gtggtcctcc tcctggttgc ccagggcggc acagaccccg ccctgggcgg
     5341 cgccggtgtg ggagcgggtg ggatagagct tggtgatgac ggcggcgtcg accccggcct
     5401 tggccagctc gagcgcggcc cagaggccgg ccccgccggc tccgacgatc aatgcgtcgt
     5461 ggttgatccg cacgttcagg tcgtcctggg aaggatgctc gggaccgtca ggagcgcgat
     5521 cgagcccatc gcgaagatca cgatcgcgag caggtagagc cccatcgtca gcacggtccg
     5581 gagaccgccg cgagtgtagt cgccgacgat gatccggagg cccatgaagg cgtggaagac
     5641 gacgaaggcg agcatcagcc agtcggcggt ccggaccgac aggttcgccc accgctcgct
     5701 gatgaacgag gagtcctgct ggaccgggtc gtagaggacg tgggtgatca ggaagtggct
     5761 gatcgcgaga acgaacagcc cgagcccggt cagccgcatc aggtaccaga cggcgagctc
     5821 gaagccgccg ccggacggac gggcgcggct gccggagcga cccaggggtc ggttggcgct
     5881 catctcagcc gccccagatc ggtttgagga t
//
LOCUS       DATAIW010000112        10335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_154424, whole genome shotgun sequence.
ACCESSION   DATAIW010000112 DATAIW010000000
VERSION     DATAIW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154424"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHM48_03765"
     CDS             complement(<1..226)
                     /locus_tag="VHM48_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX2754551.1"
                     /translation="MDQRELVRRAAKGEHEAFDTLVRHASNRLYGIAYRILRDQYLAE
                     DALQQALISIWNDLPKLRDPDRFDAWTYRVI"
     gene            complement(347..574)
                     /locus_tag="VHM48_03770"
     CDS             complement(347..574)
                     /locus_tag="VHM48_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754552.1"
                     /translation="MFNNQDLLERIEAAERVNPFCQQCGEPTIVAERDNALWLECATL
                     GHRRSRIHALLRLDFATLHTRRSIVELCAAA"
     gene            625..1275
                     /locus_tag="VHM48_03775"
     CDS             625..1275
                     /locus_tag="VHM48_03775"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2754553.1"
                     /translation="MVTRQGPGTGTFGPKRGPNGHVEVAIRPPGRPRSDVANRAILDA
                     ARDLLVEEGFTRLRLEHVAARAGVGKGTIYRRWPSKEALTLELVLDLAAPHLEITDAG
                     DTRAELVAAVRNPLEAITGTPWGPVIRAMLSQIAGNPTIGDPFRASVVARRRAEVSHV
                     IERGIARGDLRPDADASIATELLVGPVYFRLVFGGSLDAEFAERVADAVLRAFAVD"
     gene            1371..2642
                     /locus_tag="VHM48_03780"
     CDS             1371..2642
                     /locus_tag="VHM48_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2754554.1"
                     /translation="MQTVTPAEAVAGISSGDQIYLHCAAATPEVLLEALVERAPELHD
                     VGVVHLHCEGPGPHLAPEMAPHFRHRALFIGPNARAAVNSGRADYVPVFLSDVPRLFR
                     SGALPLDAVFVNATPPDAHGFCSLGTSVEAMHAAIAAARTVIVQFNSSMPRTLGESFI
                     HVDEIDYAVEVDQPPYPLHQPVIGEIEQRIGEFVADLVPDGATLQLGIGAIPSAAALH
                     LRHRKDLGVHTEMFSDAVVDLVEAGAITGALKERNRGKIVTAFLMGSQRLYDFVDDNP
                     MVEMRSVDFTNDTHVIRSFATMTAINSAIEVDVTGQVVADSIGDRFYSGVGGQMDFIR
                     GAALAEEGRAIIALPSTAANGTVSRIVSHIKPGAGVVTTRAHVRTVVTEFGVAELFGR
                     SIRERVEALIAIAHPDHRDALRFEARRTHAI"
     gene            complement(2862..4019)
                     /locus_tag="VHM48_03785"
     CDS             complement(2862..4019)
                     /locus_tag="VHM48_03785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX2754555.1"
                     /translation="MDRILVLGGGVGGTLTANLLARKLRRQLKAGTATITVVDTTGEH
                     VYQPGFMYIAMGGERAANLHRPERGLLDKRVNLVVGTVVGIDEETRLVELADGNALRW
                     DHLVLATGSRIVPESIEHFTSEAYHFYSAEAAADLRAALDRFTGGRIVIGIAGMPYKC
                     PPAPLEVSFLIESELRERGLRDKTTIDFCSPIGRAFTIESVSEMATPILAEKGIELHT
                     FFNVEAIDAERKVVLSLEGEELPYDLLILVPPHKGQQFLMDSGLAPAPGGWLPTDRET
                     LSVGGRPDVYALGDATDLPLSKAGSTAHFEAPVVAERIAAAVLGREPTGKHAEYHGKV
                     MCFFEIGDGKGTLLQFDYLHPPKPPTPNRLWHLGKIVFNKTYWHTVPKGRV"
     gene            complement(4028..4288)
                     /locus_tag="VHM48_03790"
     CDS             complement(4028..4288)
                     /locus_tag="VHM48_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2754556.1"
                     /translation="MLDATTTPAAAAASRTIDARGSFCPGPLMELIRAIRESQVGDVL
                     AVLSSDNGSRSDIPKWIEKAGHRLIGVTARDGYDEIVVEKMR"
     gene            complement(4302..4868)
                     /locus_tag="VHM48_03795"
     CDS             complement(4302..4868)
                     /locus_tag="VHM48_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX2754557.1"
                     /translation="MTLVAEPTLAPTITPPPAPVVAPQEAPIEPKTKQMVIVNWSGEL
                     DRIWPTLILSSAAAASGVQCKVFVTFWGLLPFVKDGVRITGDNWKQRMMSAMNRPGID
                     HLKLSKMNFMGMGPWMMGTLRKQYQIASPRELLEACQALGVEFIPCQMSMDMFGLKRE
                     DLIDGMADPAGAATAIEIMTEANASLFI"
     gene            complement(4878..5114)
                     /locus_tag="VHM48_03800"
     CDS             complement(4878..5114)
                     /locus_tag="VHM48_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2754558.1"
                     /translation="MELTQIDKTLDARGLACPMPIVKTAQMMKTMVSGQTLEVISTDA
                     GSVKDFAAWSRTTGNPLLESSSDAAVFRFVLRKA"
     gene            complement(5150..5563)
                     /locus_tag="VHM48_03805"
     CDS             complement(5150..5563)
                     /locus_tag="VHM48_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX2754559.1"
                     /translation="MDEPLNLILFSGTDDKLQAAAVLSAGAAALGRPVNVFLQYWALD
                     AFRADRIERSHGLAPEAGEEGRRQVEALTAAGQQSWAETFRQAKDLGGIDIEACSLSM
                     DLLGIGPADLDPLVDGVEGVTAFHLNAGNGPVVFI"
     gene            complement(5569..5988)
                     /locus_tag="VHM48_03810"
     CDS             complement(5569..5988)
                     /locus_tag="VHM48_03810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2754560.1"
                     /translation="MDEIDILQAEIMRTLANPRRLQIIHRLVGGPVEVGRIALDVGIS
                     QPNASQHLAVMRAVGVVEAERNGREVRYRLSDPEIVAACGLMRGVLARRLERLGGLSG
                     VARLPAGTRSPEAGSPVDPDAPSAPDRPLALATAPTR"
     gene            complement(6282..6683)
                     /locus_tag="VHM48_03815"
     CDS             complement(6282..6683)
                     /locus_tag="VHM48_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2754561.1"
                     /translation="MKRILLAYDGSEPAVRALETTVDLATRFGAFVGVISVVPIRAMA
                     MGVDPWDDRSVHADELRDAQDRLRQQGIEPELIEPYGDPAWTIERIAAERNFDTIVIG
                     SRGQGALGRFFSGSVSEHVATHAEATVIVTH"
     gene            complement(6884..7477)
                     /locus_tag="VHM48_03820"
     CDS             complement(6884..7477)
                     /locus_tag="VHM48_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754562.1"
                     /translation="MHQFRSIAPIPGVDASHPTREEVAASRARRPATVIRIRRITPHD
                     HDRLQEFYAGLSDESRRTRFLGPTNGIGDNQSTYFCSPDHAHREGFVAVVGPAAHPDR
                     IVGHVCVEPEGPATAEVAVAVADEMQGRGIGRKLVDAAVVWARKDGFRTLAATMLADN
                     PAIQRLLTSLGLPSVAVPIGAGVIEVRIDLGVVRSAA"
     gene            7676..8110
                     /locus_tag="VHM48_03825"
     CDS             7676..8110
                     /locus_tag="VHM48_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754563.1"
                     /translation="MATVTARRRRAIEHLVATDFARDRLARAAFDVLADGLDLGPIAI
                     RTPADRAWVRNAIAGPIQQAADHALDRLVDELIDTFAIGRPDLVARILAAAPDSPDLG
                     PGFVEWRVEFGAPFRPAARDDGALPALPAIPDGVPARVAVLR"
     gene            complement(8115..9269)
                     /locus_tag="VHM48_03830"
     CDS             complement(8115..9269)
                     /locus_tag="VHM48_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2754564.1"
                     /translation="MQPNDPNPPIDPGSTHPATPAAFNDPSTTPAPWTPSAPADATRS
                     VDPWNRPAPQSTGDAGPIGHSSADWATTAREHLGNPAGWSWRDPEPSVTAPQFEAREP
                     GQLGAPVRTPGRRSSVRTVVATALLSAVLASGSTAAIVSLVHPGSSTPAAATAPTANA
                     AVTATGGTTTVQQEDITAVVVSARDSVVTLTSQIGGGRGAFGVVGTGVGSGIILTADG
                     YALTNRHVVEGSTSLTATMADGTDYPAKVVTVSDTRDLALVKIEATGLTPATIGPSTG
                     IEVGQTAIAIGSPLGTYTETVTKGIISALDRTITVQDEQTGRPVTLSGLIQTDAAINP
                     GNSGGPLLNAIGQVVGVNTATASSAEGLGFAIPIDEAASLIAQARAASVA"
     gene            complement(9482..10222)
                     /locus_tag="VHM48_03835"
     CDS             complement(9482..10222)
                     /locus_tag="VHM48_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX2754565.1"
                     /translation="MTAVSGSNVSLATDDGWTRTIAVTTATTITKGGAAATLADIGVG
                     DVVRFSESRNADGTYAITAIEIVLPEVAGTVTAVGTDTITIALRDGTSQTIRTTGSTA
                     YQVEEADGKRADVTVGSSISATGERASDGTLTASSVWVHLPHVIGTVTVTSADTITLS
                     RRDGTTVTVHVGSDTTIRVAGVDGAKLSDVKAGMAVVVEGTQRADGSIDARAIGAGNP
                     GKGRGHDMVPGPGSDASPAPDASGSPVG"
BASE COUNT         1460 a         3525 c         3727 g         1623 t
ORIGIN      
        1 cgatcacgcg atacgtccag gcgtcgaagc ggtccgggtc gcggagcttc ggcaggtcgt
       61 tccagatgct gatcagggcc tgctggaggg cgtcctcggc gaggtactgg tcgcgcagga
      121 tccggtaggc gatcccgtag aggcggttcg acgcgtgtct gacgagcgtg tcgaacgctt
      181 catgctcgcc cttcgccgcc cggcggacga gctctcgttg atccatcgca gcccctcgat
      241 cgatgtcgcc catcgggcgt cagcgagtat gaggcgaccg gacggccggt ccttcaatct
      301 tcgtcgcgca cgccggccgc ggtggccggc cccaggtgcg ggccggtcag gccgccgcgc
      361 agagctcgac gatcgagcgg cgcgtgtgaa gggtcgcgaa gtccaggcga agcagcgcgt
      421 ggatccggct gcgacgatgg ccgagggtcg cgcactcgag ccagagcgcg ttgtcgcgct
      481 ccgcgacgat cgtcggctcg ccgcactgct ggcagaacgg gttgactcgc tccgcggctt
      541 cgatccgctc gagaaggtcc tggttgttga acatcgggat ggctccttgc catgaaacgg
      601 tacggaccgt atcgtaacat cgagatggtg acgcggcaag gtcccgggac cgggacattc
      661 ggaccgaaac gggggccgaa tggtcacgtc gaggtcgcaa ttcggcctcc tggccgccca
      721 cggagcgatg tcgccaaccg ggcgatcctc gatgccgccc gcgacctcct ggtcgaggag
      781 ggattcacgc gacttcgact cgagcatgtc gccgcccggg ccggggtggg caagggcacg
      841 atctatcgac gctggccgtc gaaggaagcg ctgaccctcg agctggtcct ggacctcgcc
      901 gctccgcatc tcgagatcac cgatgccggc gacacgcggg ccgagctcgt ggcggccgtc
      961 cgcaatccgc tcgaggcgat caccggcacg ccgtggggcc cggtcatccg ggcgatgctg
     1021 tcgcagatcg cgggcaaccc gacgatcggc gatccgttcc gggcttcggt cgtggcccgt
     1081 cgccgggcag aggtgtccca cgtcatcgag cgggggatcg cgcgcggcga ccttcgaccc
     1141 gatgccgacg cgtcgatcgc gaccgagctc ctcgtcgggc cggtctactt ccggctcgtg
     1201 ttcggcggat cactcgacgc cgagttcgcg gagcgcgtcg ccgacgccgt gttgcgcgca
     1261 ttcgcggtcg actgaccggg aggcccgggc cggatgcggg ccatcgtgac cgtccgatcg
     1321 gtcggttcgg actgtcgacc ggaccctgac cgtggtccac actgagcgac atgcagactg
     1381 tcaccccagc cgaggccgtt gccgggatca gcagcggcga ccagatctac ctccattgcg
     1441 cagccgcgac ccccgaggtc ctgctcgaag ccctcgtcga acgggcgccc gagcttcatg
     1501 acgtcggggt cgtccatctc cactgcgagg ggcccggccc gcatctcgcc ccggagatgg
     1561 ccccgcactt ccgacatcgg gcgctgttca tcggacccaa cgcgcgcgca gccgtcaatt
     1621 ccgggcgagc cgactacgtt cccgtcttcc tctcggacgt gccgcggctc ttccgatcgg
     1681 gggcgctgcc gctcgacgcg gtgttcgtca atgcgacgcc gcccgatgcc cacgggttct
     1741 gctcgctcgg cacctcggtc gaggcgatgc atgccgcgat cgccgctgcc cggacggtca
     1801 tcgtccagtt caactcctcg atgccgcgaa ctctcggcga aagcttcatc catgtcgacg
     1861 agatcgacta cgcggtcgag gtcgaccagc cgccgtaccc cctccatcag ccggtcatcg
     1921 gcgagatcga acagcggatc ggcgaattcg tcgccgatct cgtgcccgat ggggcgactc
     1981 tccagctcgg gatcggggcg atcccgtcgg cggcggccct ccacctccgc caccggaagg
     2041 acctcggcgt ccacaccgag atgttcagcg acgcagtcgt cgacctcgtc gaggccggcg
     2101 cgatcaccgg cgccctcaag gagcgcaacc ggggcaagat cgtgaccgcg ttcctgatgg
     2161 gctcgcagcg gctgtacgac ttcgtcgacg acaacccgat ggtcgagatg cgctcggtcg
     2221 acttcaccaa cgacacccat gtcatccgct cgttcgcaac gatgaccgcg atcaactcgg
     2281 cgatcgaggt cgacgtgacc ggtcaggtcg tcgccgattc gatcggggat cgtttctaca
     2341 gcggtgtcgg cgggcagatg gacttcatcc gtggcgccgc tctcgccgag gagggccgag
     2401 ccatcatcgc cctgccctcg acagccgcca acgggaccgt gtcgaggatc gtgtcgcata
     2461 tcaagccagg tgccggcgtc gtcacgacgc gcgcccacgt ccggacggtc gtcaccgagt
     2521 tcggcgtggc cgagctgttc gggcggagca tccgcgaacg ggtcgaggcg ctgatcgcga
     2581 tcgcccaccc agatcatcgc gacgccctgc gcttcgaggc ccgcaggacg cacgcgatct
     2641 gacgctcatg gcctctgggg tcagctcgac ctcgggcccg acgggtccgg gcatcccgga
     2701 cgatctctgg cgttccgggc gccgactcca aggggattct gccaccgctc cccgtgctcc
     2761 ggacgctcga cgacccgccc tgtgaccggc gggtcgtcgc cctgttggag ggccggaggt
     2821 gggaccgggc cgtcgccggc cagggtcggg gtgcgaggcc gtcagacgcg gcccttcggc
     2881 accgtatgcc agtaggtctt gttgaagacg atcttgccga gatgccagag ccgattcggg
     2941 gtcggcggct tcggcggatg gaggtagtcg aactggagca gggtgccctt cccgtcgccg
     3001 atctcgaaga aacacatgac cttgccgtga tactcggcgt gcttgccggt cggctcccgg
     3061 cccaggaccg cggcggcgat ccgctcagcg accacggggg cctcgaagtg ggctgtcgag
     3121 ccggccttgg acagcggcag gtccgtcgcg tcgccgaggg cgtacacgtc gggccggcca
     3181 ccgacgctga gcgtctcgcg gtcggtcggc agccagcctc ccggcgccgg agcgagcccc
     3241 gagtccatca ggaactgctg gcccttgtgc ggcggaacga ggatcaggag gtcgtagggc
     3301 aactcctcgc cctcgaggct gaggacgacc ttgcgctctg cgtcgatcgc ctcgacgttg
     3361 aagaacgtgt ggagctcgat gcccttctcg gcgaggatcg gcgtcgccat ctcggagacg
     3421 ctctcgatcg tgaacgcccg cccgatcggc gagcagaagt cgatcgtcgt cttgtcgcgc
     3481 aggccgcgct cgcggagctc gctctcgatg aggaacgaga cctccagcgg cgccggcggg
     3541 cacttgtagg gcatgccggc gatcccgatc acgatccgcc cgcccgtgaa ccggtcgagg
     3601 gcggcccgca gatcggcggc ggcctcggcg ctgtagaagt ggtaggcctc cgaggtgaag
     3661 tgctcgatcg actcgggcac gatccgcgac ccggtggcga ggaccaggtg gtcccagcgc
     3721 agcgcgttgc catcggccag ctcgacgagc cgcgtctcct cgtcgatccc gacgaccgtc
     3781 ccgacgacga ggttcacccg cttgtcgagg agaccgcgct cgggacggtg gaggttggcg
     3841 gcgcgttcgc cacccatcgc gatgtacatg aagccgggct ggtagacgtg ctcgccggtg
     3901 gtgtcgacga cggtgatcgt cgcggtcccg gccttgagct ggcggcggag cttgcgggcg
     3961 aggaggttgg cggtcagcgt gccgccgacc ccgcccccga ggaccaggat gcgatccatc
     4021 gggttcatca acgcatcttc tcgacgacga tctcgtcata gccgtcccgg gcggtcacgc
     4081 cgatcagccg gtgaccggct ttctcgatcc acttcgggat gtcgctccgg ctgccgttgt
     4141 cggaggagag gaccgcgagg acgtcgccga cctgcgactc gcggatcgcc cggatgagct
     4201 ccatgagggg cccggggcag aagctgccgc gggcgtcgat cgtgcgggag gccgctgcgg
     4261 cggcaggggt ggtggtcgcg tcgagcatcg ctcgttcctt cctagatgaa gagcgaggcg
     4321 ttggcctcgg tcatgatctc gatcgcggtc gcggcgccgg ccgggtcggc catgccgtcg
     4381 atcaggtcct cgcgcttgag accgaacatg tccatcgaca tctggcacgg gatgaattcg
     4441 acgccgaggg cctggcaggc ctcgagcagc tcgcgcggcg aggcgatctg gtactgcttg
     4501 cggagcgttc ccatcatcca cgggcccatg cccatgaagt tcatcttgct gagcttgaga
     4561 tggtcgatcc cggggcggtt catggcggac atcatccgct gcttccagtt atcgccggtg
     4621 atccggacgc cgtccttgac gaacggcagg aggccccaga aggtcacgaa gaccttgcac
     4681 tggacgccgg acgccgcggc ggcggacgac aggatgagag tcggccagat ccggtcgagc
     4741 tcgcccgacc agttgacgat gaccatctgc ttggtcttcg gctcgatcgg agcttcctgg
     4801 ggcgcgacga cgggtgccgg cgggggggtg atggtcggtg cgagcgtggg ctctgcgacg
     4861 agggtcatcg tggtcactca ggccttccgg aggacgaagc ggaagaccgc tgcgtcggag
     4921 ctggattcga ggagcgggtt gccggtcgtc cgcgaccagg cggcaaagtc cttgacggag
     4981 cccgcatcgg tcgagatcac ctcgagcgtc tggcccgaga ccatcgtctt catcatctgg
     5041 gccgtcttga cgatgggcat cggacaggcc agccctcggg catcgagggt cttgtcgatc
     5101 tgggtgagtt ccatgattgc ctccttcggc agtcgatcgc gctgggcggt cagatgaaga
     5161 cgaccggtcc gttgccggcg ttgagatgga acgcggtcac gccctcgacg ccatcgacga
     5221 gcggatcgag gtcggctggg ccgatcccga gcaggtccat cgagagcgag caagcctcga
     5281 tgtcgatccc gccgaggtcc ttcgcctggc ggaacgtctc ggcccagctc tgctggccgg
     5341 ccgccgtgag cgcctcgacc tgccgccggc cctcctctcc ggcctccggc gcgaggccgt
     5401 gggaccgctc gatccggtcc gcccggaacg cgtcgagcgc ccagtactgg aggaagacgt
     5461 tcaccggccg gccgagcgcg gcggcgccgg cgctgagcac cgcggccgcc tgcagcttgt
     5521 cgtccgtgcc ggagaacagg atcaggttca ggggctcgtc catctggttc acctcgtggg
     5581 ggccgtcgcc agggcgagcg gccgatcggg agcggaaggg gcgtccggat cgaccggcga
     5641 gccggcctcc ggggaacggg ttccggcggg cagccgggca acgcccgaca gcccgcccag
     5701 gcgttcgagc cggcgggcga ggacgccgcg catcagcccg caggcggcaa cgatctcggg
     5761 atcgctgagt cggtagcgga cttcgcgtcc gttgcgctcg gcctcgacca caccgaccgc
     5821 gcgcatgacc gcgaggtgct gggaggcgtt cggctggctg atcccgacat cgagggcgat
     5881 ccgcccgact tcgaccgggc cgccgacgag gcggtggatg atctggagcc ggcggggatt
     5941 ggcgagcgtt cgcatgatct cggcctggag aatgtcgatc tcgtccatga ctggagcatc
     6001 gactcatgcg cacatgcgca ggagtgccat cccggcccaa gacggcgggc cgaacggccc
     6061 atcgttcgaa cggacctcct cggcccagcc cggcgggccc tccgagcgat cgtcggttca
     6121 ggtcccggcg gcgtccggcc gggacgccat cacgacccgc aggcgcgacg acgccgcccg
     6181 cgactggcgc ggacggcgtg gggctcgtcg ctcctccgtc gggtcgtgcc cggggcggat
     6241 cgaaatccgc gggctcgggc gcaggaccct ggttgtgggg ttcagtgggt cacgatcacc
     6301 gtggcttcgg cgtgcgtcgc gacatgctcg ctgacactgc cgctgaagaa ccgaccgagg
     6361 gcgccctggc cgcgcgagcc gatcacgatc gtgtcgaagt tccgctcggc cgcgattcgc
     6421 tcgatcgtcc aggcggggtc gccgtacggt tcgatgagct ccggctcgat cccttgctgc
     6481 cggaggcgat cctgggcgtc gcgcagctcg tccgcatgga cgctgcgatc gtcccacggg
     6541 tcgacgccca tggccatggc ccggatcggg acgacgctga tgacgccgac gaaggctccg
     6601 aaccgcgttg ccaggtcgac ggtcgtttcg agggcccgaa cggcgggctc gctgccgtca
     6661 taggccaaca ggatgcgctt catggtcgac ctctctttcg ttccggcgcg gtcgcacaac
     6721 ggcggctcgt gccggatctg actcgagcat cgtcccggcg cggcccggtg gcgaaggtcc
     6781 gaggtcatgc gattgcaggc cgaacggacc attcggcgcc cggaccattc ccgaggccga
     6841 gaccaccttc gagcccgcgg agatcccggg ttcggccggc cggtcaggcg gcggagcgca
     6901 ccacgccgag gtcgatccgg acctcgatca cgccggctcc gatcgggaca gccacgctcg
     6961 gcaggccgag gctggtcagc agtcgctgga tcgccgggtt gtcggcgagc atcgtggcgg
     7021 ccaacgtccg gaacccgtcc ttgcgcgccc acacgaccgc ggcatcgacc agctttcgac
     7081 cgatcccgcg tccctgcatc tcgtccgcga cggcgaccgc gacctccgcg gtcgccggtc
     7141 cttccggctc gacgcagacg tggccgacga tccggtcggg gtgggccgcc ggtccgacga
     7201 ccgcgacgaa gccctcgcga tgggcgtgat cgggcgagca gaagtaggtc gactggttgt
     7261 cgccgatgcc gttcgtcggc ccgaggaagc gcgtccgccg gctctcgtcg gaaaggcctg
     7321 cgtagaactc ctgcaggcgg tcgtggtcgt gcggggtgat gcgccggatg cggatcacgg
     7381 tcgcgggccg tcgcgcccgg gacgccgcca cttcctcgcg cgtggggtgc gacgcatcga
     7441 cgcccggaat cggagcgatc gaccggaact ggtgcatggg ctgacggtac ccgccggcgc
     7501 ccggtccggc gagaggccca acggcccggc cgcagggtcc gatcggatcg aacggccgat
     7561 cgacgtcgca cggagggccg aacggctcat ggcggcggtg ccccggaggc gtagcgtgat
     7621 ggcccgcgca acgggcccac ggacccgagc gacgcggcga ggaggacgga acgacatggc
     7681 gaccgtgacg gcccgccgac ggcgtgccat cgagcatctc gtggcgaccg acttcgcccg
     7741 ggatcggctc gcccgcgcgg cgttcgacgt cctggcggat ggcctcgacc tggggccgat
     7801 cgccattcgc acgccggcag atcgagcgtg ggtccggaac gcgatcgccg gcccgatcca
     7861 gcaggccgcc gaccatgcac tcgaccggct cgtcgacgaa ttgatcgaca cgttcgccat
     7921 tggccgcccg gacctggtcg cccggatcct tgcagccgct cccgacagcc cggatctcgg
     7981 tccgggattc gtcgaatggc gggtcgagtt cggcgcgccg ttccggcccg ccgcgcggga
     8041 cgacggcgcg ctcccggcgc tcccggcgat cccggacggg gttcctgccc gggtcgcggt
     8101 ccttcgctag ccgatcaggc gaccgacgcc gcccgagcct gggcgatcaa gctcgccgct
     8161 tcatcgatcg ggatcgcgaa gcccagaccc tcggcgctcg acgcggtcgc ggtgttgacg
     8221 ccgacgacct ggccgatcgc gttgaggagc gggccaccgc tgttgccggg gttgatcgcg
     8281 gcatcggtct ggatcaggcc gctgagcgtg accggccgac ccgtctgctc gtcctgaacg
     8341 gtgatcgtcc ggtcgagcgc ggagatgatc cccttggtca cagtctcggt gtaggtgccg
     8401 agcgggctgc cgatggcgat cgcggtctgg ccgacctcga tcccggtcga cgggccgatc
     8461 gtcgccggcg tgagaccggt ggcctcgatc ttgacgaggg ccaggtcacg ggtgtccgag
     8521 acggtgacga ccttcgcggg atagtccgtg ccgtcggcca tggtcgcggt cagcgacgtc
     8581 gacccctcga cgacatggcg attggtcagg gcgtacccgt cggcggtgag gatgatcccc
     8641 gagccgaccc cggtcccgac gacgccgaat gctccgcgcc ccccgccgat ctgcgacgtg
     8701 agcgtcacga ccgaatcgcg ggccgacacg acaacggcgg tgatgtcctc ctgctggacc
     8761 gtcgtggtcc cgccggtcgc ggtcacggcg gcgttcgccg tcggggccgt cgcggcggcc
     8821 ggggtcgatg aacccggatg gacgaggctg acgatcgccg ccgtcgatcc ggatgcgagc
     8881 acggccgaca ggagcgccgt cgcgacgacg gttcgaaccg agctgcggcg accgggcgtc
     8941 ctgaccggag cgccgagctg gcccggctcg cgagcttcga actgcggcgc ggttacggag
     9001 ggctccgggt cccgccacga ccagccggcc ggattcccga ggtgctcgcg agccgtggtc
     9061 gcccagtcgg cggacgagtg cccgatcgga ccggcgtcgc cggtcgactg cggggctggt
     9121 cggttccatg gatcgacgct gcgggtcgca tccgccgggg cggacggcgt ccatggggcg
     9181 ggcgtcgtcg atggatcgtt gaacgcggcc ggggtcgcgg gatgagttga ccctgggtcg
     9241 atcggcgggt tggggtcgtt cggctgcatc gcgggtggtc tccggtcgcg gacgggcgga
     9301 tcgtgtcacg tctgatggaa ccgcgtggga ctgagaaggt cctgagatgc cacgccatgc
     9361 gggcgggtca ccgcgatcgt cgtttggggt cacggtcttt ccgcggtcgg gcgctggcgc
     9421 gggaacgcgc gggaccgaac tggtcccgcg cgtcccggcg ctgcgaatgg atcccgatgg
     9481 atcagccgac ggggctgccg gaagcgtcgg gcgcgggcga ggcgtccgaa cccgggccgg
     9541 gcaccatgtc gtggccgcga cccttgcccg gattgccggc gccgatcgcg cgggcgtcga
     9601 tcgagccgtc ggcccgctgg gtgccctcga cgacgacggc catgccggcc ttgacgtccg
     9661 acagcttggc gccgtcgacg ccggcgaccc ggatcgtcgt atctgagccg acgtgaaccg
     9721 tgaccgtcgt cccgtcgcgc cggctgagcg tgatcgtgtc ggcgctcgtg accgtcacgg
     9781 taccgatcac gtgcggcagg tggacccaga cggagctcgc cgtcagcgtg ccgtcggagg
     9841 ctcgttctcc ggtcgcggag atcgacgagc cgacggtcac gtcagcgcgc ttgccgtccg
     9901 cttcctcgac ctgataggcg gtggagcccg tcgtcctgat cgtctgcgag gtgccgtcgc
     9961 gcagggcgat ggtgatcgtg tccgtgccga ccgcggtcac cgtcccggcg acctcgggca
    10021 ggacgatctc gatcgccgtg atcgcgtacg tcccgtcggc gttgcggctc tcggagaagc
    10081 ggacgacgtc gcccacgccg atgtcggcga gcgtggccgc ggccccgccc ttcgtgatcg
    10141 tggtagcggt cgtgacggcg atggtgcgcg tccagccatc gtccgtcgca agcgacacgt
    10201 tcgatccgga gaccgcggtc acggacacct gtccgaaccc gcggaccccc atgcggccag
    10261 gccaagggac cgggcgggcc cggccgcatg ggggtccgcg ggttcggaca ggtgtccgtg
    10321 accgcggtct ccgga
//
LOCUS       DATAIW010000113         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_94822, whole genome shotgun sequence.
ACCESSION   DATAIW010000113 DATAIW010000000
VERSION     DATAIW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94822"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VHM48_03840"
     CDS             <1..573
                     /locus_tag="VHM48_03840"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2754566.1"
                     /translation="AGHERRLHRGPARVFDSEGECYAAVRDRRIVAGDVVVIRYEGPV
                     GGPGMQEMLSVTGALVGEGLGDTVALITDGRFSGGTHGLMIGHIAPEAALGGPIGLVE
                     EGDEIAIDVDARRLDLAVPAEILEQRRGHWTAPAPRYTSGVMAKYAALVSSASEGAVT
                     TGRRMTEALQRTGAADSAASAFGDAAVTAG"
     gene            570..1469
                     /locus_tag="VHM48_03845"
     CDS             570..1469
                     /locus_tag="VHM48_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2754567.1"
                     /translation="MTVRIGFKTSPQDVDWATLDATWARAGELAGGPDGEFDSGWLND
                     HLTNLDVAAPGPSLEALTLLAALVHHVPGMTVGHAVLSNTFRHPMLLAKAATVLDHAT
                     GGRFVVGLGAGWFDGEHVPFGIDLPPIGPRIDRLVSAVDTLRALFSAEAAVAPGVTRP
                     DPFYPLAGAVNAPPPLTPGGPPLYLGGQGPRGIRLAARAAAGWLLPGTHAGNVPYFAG
                     KRAELLDALAAEGRDRGGFDFVGQVSTGLTATSRREAVEAAIGLVGAGATHLILGMPA
                     ALGPDGLDEVVRDCLRPLRDRLG"
     gene            1508..1753
                     /locus_tag="VHM48_03850"
     CDS             1508..1753
                     /locus_tag="VHM48_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754568.1"
                     /translation="MTVPPDPKAYDTERNAAARKRGLSTPYIPGGRDPDQAHAEAQDR
                     RYLRILLIMVVVIVLGGFVVGILAALAGLDALVGGSG"
     gene            1756..3189
                     /locus_tag="VHM48_03855"
     CDS             1756..3189
                     /locus_tag="VHM48_03855"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2754569.1"
                     /translation="MPTTHILPSTTLADGVLERASEELVETLRSLIRIPSINPVPADT
                     PDGETRAAHWIAAALADAGVVPEVLEAVPGRGSVFARLRGDDSGGEPLLLLSHLDVVP
                     APAEGWTHDPFSGDVAADYVWGRGAVDMKNLLAMELTVVRMLAAEARAAGRDPATDPI
                     PGLTRDVLFASTADEEAGGLNGIGWIVAERPDLIRAAAAINESGAVATTFGDRRFYPI
                     GVAEKGYAVYRLTVRGTWGHGSMPRDDNAAVRAATAVTRLAVQGGPRPTEVMQTFLGG
                     VADQLDGEHARLLRALASDDPRLGEAALDAICDPMPARVVRALLRDTVSPNIIHAGVK
                     YNVIPGEATIELDCRLLPGTTEADMRETIVGRLGDLAAHIDVDLVIHAPPVAAPAHGD
                     FYDLLAATIVDHDPAGIPLPFMVPFATDAKHTAALDVPTYGFSPLRLDPEERFMERFH
                     GVDERVGIDALRWGLPVLYDVVRRYCG"
     gene            complement(3278..>3556)
                     /locus_tag="VHM48_03860"
     CDS             complement(3278..>3556)
                     /locus_tag="VHM48_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754570.1"
                     /translation="SPSATSRLVDGLVRRRLVERRVEEEDRRQRTLQLTPRGAALLRV
                     VDRARADQFLSAVRPMPTAERALIAMGVAALATHAISRRGRLIKNRTE"
BASE COUNT          467 a         1359 c         1241 g          489 t
ORIGIN      
        1 gcgggtcacg agcggcgact ccaccgcggt cctgcccggg tgttcgactc cgagggcgag
       61 tgctacgcgg cggttcgcga tcgccggatc gtcgccggtg acgtcgtcgt catccgctac
      121 gagggtcccg tcggtgggcc cgggatgcag gagatgctga gcgtgacggg tgccctcgtg
      181 ggcgagggac tgggcgacac ggtggccttg atcaccgacg gccggttctc gggcggcacg
      241 catggcctga tgatcggcca catcgcgccc gaagccgccc tcggcggacc gatcggcctc
      301 gtcgaggagg gcgacgagat cgccatcgac gtcgacgccc ggcgcctgga ccttgccgtc
      361 ccggccgaga tcctcgagca gcggcgcggc cactggacag cgcccgcgcc gcgctacacg
      421 agcggcgtca tggccaagta cgcagcgctc gtctcgtcgg cttccgaggg cgcggtgacg
      481 accggccgac ggatgacgga agccctccag cgcacgggcg ctgccgactc ggcggcgagc
      541 gcgttcggcg acgcggcggt cacggccgga tgacggtccg gatcgggttc aagacctcgc
      601 cgcaggacgt cgactgggcc acgctcgatg ccacctgggc gcgggccggc gagctggccg
      661 gcgggccgga cggcgagttc gacagcggct ggctcaacga ccacctgacg aacctggatg
      721 tggccgcgcc ggggccgtcg ctcgaggcgc tgaccctgct cgcggcgctc gtccatcacg
      781 tcccggggat gaccgtcggc cacgcggtcc tgtcgaacac cttccgccat ccgatgcttc
      841 tcgcgaaggc cgccaccgtc ctcgaccacg cgaccggcgg gaggttcgtc gtgggcctcg
      901 gggccggctg gttcgacggc gagcacgtgc cgttcgggat cgacctgccg ccgatcggcc
      961 cgcggatcga ccggctcgtc tcggcggtcg acaccctccg ggcgctcttc tccgcggagg
     1021 ccgcggtggc gcccggggtg acccgtccgg atccgttcta cccgctggcc ggtgccgtca
     1081 acgcgccgcc gccgctcacg cccggcggcc cgccgctgta cctcgggggc cagggcccac
     1141 gcggcatccg gctcgcggca cgagccgccg ccggctggct cctgcccgga acccacgccg
     1201 gcaacgtccc ctactttgcc gggaagcggg ccgagctcct cgacgccctc gcggccgaag
     1261 gacgggaccg cgggggcttt gacttcgtcg gccaggtctc gaccgggctg acggcgacaa
     1321 gcaggcgcga ggcggtcgag gcggccatcg gcctcgtcgg cgccggcgcg acgcacctca
     1381 ttctcggcat gccggcggcc ctcggccccg acggactcga cgaggtcgtc cgcgactgcc
     1441 ttcggcccct gcgcgatcgc ctcggatgat ccatgcgcga ccccgccgat cgggcgatga
     1501 tgacgccatg accgtcccgc ccgatccaaa ggcgtacgac acggagcgca atgcagccgc
     1561 ccgcaaacgc ggcctctcga cgccgtacat cccgggcggt cgggaccccg accaggcgca
     1621 cgccgaggcc caggatcgcc gctatctccg gatcctgctg atcatggtcg tcgtcatcgt
     1681 cctcggcggg ttcgtcgtcg ggatcctcgc cgcacttgcc gggctcgacg ccctcgtggg
     1741 cggctccggc tgacgatgcc gacgacgcac atcctgccct cgacgaccct cgccgacggc
     1801 gtcctggaac gggcgagcga ggagctcgtc gagactcttc gatcgctgat ccggatcccc
     1861 tcgatcaacc cggtgcccgc cgacacgcca gacggcgaga cacgcgcggc gcactggatc
     1921 gccgccgccc tcgccgacgc cggggtcgtg ccggaggtcc tcgaggcggt gcccggccgc
     1981 ggctcggtct tcgcccgcct ccgcggcgac gatagcggcg gcgagccact cctgctcctc
     2041 agtcacctcg acgtcgtgcc cgcgcccgcc gaggggtgga cccacgaccc gttcagcggc
     2101 gatgtggccg ctgactacgt ctggggtcgc ggcgccgtcg acatgaagaa cctccttgcc
     2161 atggagctca cggtcgtccg gatgctggcc gccgaggccc gcgccgccgg ccgcgacccc
     2221 gcgacggacc cgatccccgg tctgacccgc gacgtcctct tcgccagcac ggcggacgag
     2281 gaggcgggcg gtctcaacgg catcggctgg atcgtcgccg aacgcccgga tctgatccgg
     2341 gcggccgctg cgatcaatga atccggcgcg gtcgcgacca cgttcggcga ccgccgcttc
     2401 tacccgatcg gcgttgcgga gaagggctac gcggtctatc ggctgaccgt ccgcgggacg
     2461 tggggccacg gctccatgcc gcgtgacgac aacgccgccg ttcgtgcggc gacggcggtg
     2521 acccggctgg cggtccaggg cggaccgcgc ccgaccgagg tgatgcagac attcctcggc
     2581 ggtgtcgccg accagctcga cggcgagcac gcccggctcc tgcgggcgct cgcgagcgac
     2641 gaccctcgac tcggcgaggc ggcccttgac gccatctgcg atccgatgcc cgctcgcgtg
     2701 gtccgggctc tcctgcgcga caccgtcagc ccgaacatca tccacgccgg ggtgaaatac
     2761 aacgtcatcc cgggcgaggc gacgatcgag ctcgactgcc ggctgctgcc gggcacgacc
     2821 gaggccgaca tgcgcgagac gatcgtcggg cggctcggcg acctggccgc ccacatcgat
     2881 gtcgatctcg tgatccacgc gccaccggtc gcggctccgg cgcacggcga cttctacgac
     2941 ctcctggcgg ccacgatcgt cgaccacgac ccggccggga tcccgctccc gttcatggtc
     3001 ccgttcgcga ccgacgccaa gcacaccgcc gcgctcgacg tcccgaccta cggcttctcc
     3061 ccgctccgtc tcgaccccga ggaacggttc atggagcgct tccacggggt cgacgaacgg
     3121 gtcgggatcg acgcgctgcg ctggggattg cccgtcctgt acgacgtggt ccgccgctac
     3181 tgcggctgat cggacccggc cgggcgctcc gcggaacgcg gttcagcggc gcgcatcgtc
     3241 gcagcatcca gggcgacccg gcatgcccga cccgctgcta ctccgtgcgg ttcttgatca
     3301 agcgtccgcg gcgcgagatc gcgtgggtcg ccagggcggc gacgcccatc gcgatcagcg
     3361 cccgctccgc agtcggcatc ggtcggacgg ccgacaggaa ctgatccgcc cgagcccgat
     3421 cgaccacccg cagaagcgcc gcccctcgcg gcgtcagctg gagcgtccgc tggcggcggt
     3481 cctcctcctc gacccggcgc tccacgaggc gtcgccggac cagcccgtcc accagccggc
     3541 tcgtcgccga cggtga
//
LOCUS       DATAIW010000114         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_270621, whole genome shotgun sequence.
ACCESSION   DATAIW010000114 DATAIW010000000
VERSION     DATAIW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270621"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1293)
                     /locus_tag="VHM48_03865"
     CDS             complement(235..1293)
                     /locus_tag="VHM48_03865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX2754571.1"
                     /translation="MPQTPQEFRMLTQKSSAADAAATSPAPAGPNATAASDPHAAPAP
                     RDLEDAIVYFEGNFVPMRDAKVSIMTHAFMYGTATFEGIRAYWNEDQGKLYGVKIREH
                     VERIRQSCRILIMENVPSVDELTRLIVETIRRNRFREDAYIRPSFYKSTKAIGVRLHH
                     LENELYIIAIPFGNYVDTDTGIRVMTSSWRRNADEALPARGKIVGGYVNMAFQKSEAE
                     MNGYDEAVVLTADGHVNEASAANLFIVRDGVALTPPVNDDILEGVTRLAMIELLSNEK
                     IPVEVRSIDRSELYVADEMFLCGTGVQISPVTEIDHRPIGSGEIGPITSLVKERYFDA
                     VRGRLPAYAHWLTEIPAS"
     gene            complement(1349..>2355)
                     /locus_tag="VHM48_03870"
     CDS             complement(1349..>2355)
                     /locus_tag="VHM48_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754572.1"
                     /translation="AELFTNGIEWFGRKLELAEGAVGSVLAAVGTALPETMIPIIAIL
                     SGGGSAASHGIGVGAILGAPFMLSTLAMFVTGVAVMYAGRKRPKGDDMPVDTTVLAHD
                     MRYFGIAYAIAVAAAFLPLEPAWPKWIVAVVLIGIYGWYVKGHFEDDPDVDAADLAPL
                     RFNRLDPATPNGEGVVPRLRVVNLQVASALGLIVIGAFFFVAAVEHLSESFGIAPIIV
                     ALVIAPIATELPEKFNSVIWVRQGKDTLAMGNITGAMVFQSTIPTVVALLIAADAWHF
                     SADSRVAFASAGVAFIASAVIFIPMARSGRLLGRRLLVGGAFYVVYLGIVAASLAGLF
                     "
BASE COUNT          394 a          769 c          843 g          349 t
ORIGIN      
        1 tcagcggtga gcgcgctcgg catcgtcgga gttgcgaccg agccacgcac gctgaagccc
       61 gcgctgactc gccgggtgag tcagccgcca gccgcgacag ccgcgtgact gcgttcgtcg
      121 cgctggggcg aggagggcaa ggcgccgccg aggaccgcag caaagcaggc tcggatgccc
      181 gtaagcgagg accgcaggcc gcaacgccgc catcagcgtt ccagcgcgac agccctagga
      241 ggcggggatc tcggtgagcc agtgggcgta cgccggcagg cggccgcgga cggcgtcgaa
      301 gtagcgctcc ttcacgagcg acgtgatcgg gccgatctcg cccgagccga tcgggcggtg
      361 gtcgatctcg gtgaccggcg agatctggac accggtcccg cagaggaaca tctcgtcggc
      421 cacgtacagc tcggagcggt cgatcgagcg aacctcgacc gggatcttct cgttgctgag
      481 gagctcgatc atcgcgaggc gggtcacgcc ctcgaggatg tcgtcgttga ccggcggggt
      541 cagggcgaca ccgtcacgga cgatgaacag gttggccgcc gaggcctcgt tgacgtggcc
      601 gtcggccgtg aggacgaccg cctcgtcgta gccgttcatc tcggcctcgg acttctggaa
      661 ggccatgttc acgtagccgc cgacgatctt gccgcgcgcc ggcagggcct cgtccgcgtt
      721 gcgccgccag gatgaggtca tcacccggat cccggtgtcg gtgtcgacgt agttcccgaa
      781 cgggatcgcg atgatgtaca gctcgttctc gaggtgatgg aggcggaccc cgatcgcctt
      841 cgtcgacttg tagaacgaag gccggatgta ggcgtcctcg cggaaccgat tgcgccggat
      901 cgtctcgacg atgagacggg tcagctcgtc gaccgacggc acgttctcca tgatgaggat
      961 ccggcaggac tgccggatgc gctcgacgtg ttcgcggatc ttgacgccgt agagcttgcc
     1021 ctggtcctcg ttccagtacg cccggatgcc ctcgaaggtc gcggttccgt acatgaacgc
     1081 gtgggtcatg atgctgacct tcgcgtcgcg catcgggacg aagttgccct cgaagtagac
     1141 gatcgcgtcc tcgaggtcgc gcggggccgg ggcggcatgc ggatcggagg cggccgttgc
     1201 gttcgggccg gcgggggccg gcgacgtggc cgcggcgtcg gccgcgctcg acttctgggt
     1261 cagcatgcgg aactcctgcg gggtctgcgg cacgggtgcc gggggatcgt ctgacggtcc
     1321 gccgagtata tcagcggcct ccgaccgctc agaacaggcc ggccagcgac gccgcgacga
     1381 tcccgaggta gacgacgtag aacgccccgc cgacgagcag gcgacggccg agcagccgtc
     1441 cggacctggc catcgggatg aagatcacgg ccgaggcgat gaacgcgacc ccggccgacg
     1501 cgaaggcgac ccggctgtcg gcgctgaaat gccacgcatc cgccgcgatc agcagggcga
     1561 cgaccgtcgg gatcgtcgac tggaagacca tcgcccccgt gatgttgccc atcgccaggg
     1621 tgtccttgcc ctgacggacc cagatgacgg agttgaactt ctcgggcagc tcggtcgcga
     1681 tcggggcgat gacgagggcc acgatgatcg gggcgatgcc gaacgactcg gacaggtgct
     1741 cgaccgcagc gacgaagaag aacgctccga tcacgatcag cccgagtgcc gaggcgacct
     1801 ggaggttgac gacgcgcaac cgcgggacga ctccttcgcc gttcggcgtg gccgggtcga
     1861 ggcgattgaa gcggagcggc gccaggtccg cggcgtcgac gtccgggtcg tcctcaaagt
     1921 ggcccttgac ataccagccg tagatcccga tcaggacgac cgccacgatc cacttcggcc
     1981 aggcgggctc gagcgggagg aaggcggccg cgacggcgat cgcataggcg atcccgaagt
     2041 agcgcatgtc gtgggcgagc acggtcgtat cgaccggcat gtcgtcgcct ttcgggcgct
     2101 tgcgcccggc gtacatcacc gcgacgccgg tgacgaacat cgcgagggtc gagagcatga
     2161 acggcgcgcc caggatcgcc ccgacgccga tcccgtggga cgctgccgac ccgccgccgg
     2221 acagaatcgc gatgatcggg atcatcgtct cgggcaaggc cgtcccgacc gccgcgagga
     2281 ccgacccgac cgcaccttcg gcgagctcca gcttgcgccc gaaccactcg atgccgttgg
     2341 tgaagagctc ggcac
//
LOCUS       DATAIW010000115         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_154644, whole genome shotgun sequence.
ACCESSION   DATAIW010000115 DATAIW010000000
VERSION     DATAIW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154644"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VHM48_03875"
     CDS             <1..445
                     /locus_tag="VHM48_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2754573.1"
                     /translation="ALDSDQYQKKLQTGEAVLGLTWTGGLDELIQDPKTADTKYIIPS
                     DGTLYWLDTWVIFKDPPHPIAAHAFLNFIEEPAIQAQETVTNAYATPNDEAKKLVPQK
                     ILDDPTIFVPQAIFDSGVLESAKDVSTNKLRVQIWAEFKSKIGKK"
     gene            468..1337
                     /locus_tag="VHM48_03880"
     CDS             468..1337
                     /locus_tag="VHM48_03880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754574.1"
                     /translation="MTTRVSRRSSLLTGVLLLPASLFYLLLLVAPIIIVVVFSFGYSG
                     ETGGYAGGFTTANYEDLIANNIDPLVTSLRLSVFGTILCLLVGLPLAYYIATRAGRRK
                     GLFIVLLVVPFWTSFLIRTYAWLTILGPEGLAGFVGNIVGDPTFRILGTEWAVQLGLV
                     YGYLPLMVFPLYVTLERMDRSLVEASKDLGAGRWATFRQITLPIALPGLITGSILVFI
                     PMMGEYVIPNILGYGRVFTVGSSLVLRFLEARDWPSGSAQAVVLVAIMLVSVTFYVWF
                     INRGRRGREVSML"
     gene            1375..2337
                     /locus_tag="VHM48_03885"
     CDS             1375..2337
                     /locus_tag="VHM48_03885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754575.1"
                     /translation="MSAPAIPAAPLAAGREMPRGRRPARDRSGVLVEIWLRGHTWLVY
                     GFLYLPILVVVVFSFNASTRVTNWTGFSLHWYEFVLASKEIQRNLINSLIVGVATATV
                     STIVGTIAALGIQRAPKWFKLPFDALTYVSLIVPEIVIALSTLVFFAVTIGRGGLLSN
                     LTGGPEIGLGYHTIVGALSVFNISLVLLLVQARLSNMDRTHVEASFDLYGTPWRTFWQ
                     ITFPQLLPAIVAGYLLSFTFAFDDYIITSFVHGTNQTTIPLYVFGALRKGVNPSTNAV
                     AALMLFVTLAILVVGQFLLSRQARKSGAAGPAGVAGMIVEQQGG"
     gene            2339..2830
                     /locus_tag="VHM48_03890"
     CDS             2339..2830
                     /locus_tag="VHM48_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX2754576.1"
                     /translation="MTGEPRDSGDPFAIDNVDAWRGPTASQAAGLTLAAARAIVAAVL
                     AEARTLRVALSCAVVDSGDQLVAFERMDGADLLGITLAKDKAFTALTNHMPTADLPPI
                     TQPGTEFFGYHTIAGGRTIIFAGGMPLERGGVLVGAVGVSGGDSAQDQQAVEAGVRAF
                     GGS"
     gene            complement(3123..4034)
                     /locus_tag="VHM48_03895"
     CDS             complement(3123..4034)
                     /locus_tag="VHM48_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2754577.1"
                     /translation="MSGSAAPTPALAVFLSAIESFDRRLRAIDDRSLEAFWAWEAYDE
                     EGVRFGILRTTEQVADAAIEIAARRVEVAAAPGRAGRILGRYLVAWRELWSVADRADA
                     ARDPALDTAPLEGEWPLRSILDHLIEADLGFLVTIRNGLDQRRSGIGEPERVGSDEAW
                     IALAEVDEPSWRAAIAGSLDDIRAFHRSGRDRIVARLAGMTDDELAVSSPFWDGDRPN
                     RFRLGRFESHLRQHTIQAEKAVQATIGAPREVERLLRLLARATGDVEAAAIGADPGLV
                     EPILGPLVAEIGERTDQLVDATSLAAD"
BASE COUNT          618 a         1475 c         1369 g          652 t
ORIGIN      
        1 ggcgctcgac tccgaccagt accagaagaa gctccagacc ggcgaggccg tgctcgggct
       61 cacctggacg ggcggcctcg acgagctgat ccaggacccg aagacggccg acacgaagta
      121 catcatcccg tccgacggga cgctgtactg gctcgatacc tgggtcatct tcaaggatcc
      181 gccgcacccg atcgccgccc acgcgttcct caacttcatc gaggagccgg cgatccaggc
      241 ccaggaaacg gtgacgaacg cctacgccac gccgaacgac gaggcgaaga aactcgttcc
      301 gcagaagatc ctcgacgacc cgacgatctt cgttccgcag gcgatcttcg actcgggcgt
      361 cctcgagagc gccaaggacg tctcgaccaa caagctccgg gtccagatct gggcagagtt
      421 caagtccaag atcggcaaga agtagcccgt agccgggaac cgttccgatg acgacccgcg
      481 tcagcaggcg ctcgagcctg ctgaccggcg tcctgctgct ccccgcgagc ctcttctatc
      541 tgctgctgct cgtcgccccg atcatcatcg tcgtggtctt cagcttcggc tattccggcg
      601 aaaccggcgg ctacgccggc ggattcacga ccgccaacta cgaagacctg atcgcgaaca
      661 acatcgaccc gctcgtgacg agcctgcggc tgtcggtgtt cggaacgatc ctgtgcctgc
      721 tcgtcgggct cccgctcgcc tactacatcg cgacccgcgc cggccggcgc aagggcctct
      781 tcatcgtcct gctcgtcgtc ccgttctgga cgagcttcct gatccggacc tatgcctggc
      841 tgacgatcct cggacccgag ggcctggcag gattcgtcgg caacatcgtg ggcgacccca
      901 cattccgcat cctcggcacc gaatgggccg tgcaactcgg gctcgtctac ggctatctgc
      961 cgctcatggt gttcccgctc tacgtcaccc tcgaacggat ggaccgaagt ctcgtcgagg
     1021 cgtcgaagga cctcggcgcc gggcgctggg cgaccttccg ccagatcacc ctgccgatcg
     1081 cgctgcccgg actgatcacc ggcagcatcc tcgtcttcat cccgatgatg ggcgagtacg
     1141 tcatcccgaa catcctcggc tacggccggg tgttcaccgt gggcagctcg ctggtcctcc
     1201 ggttcctcga ggcccgcgac tggccgtcag ggtcggccca ggcggtcgtc ctcgtcgcca
     1261 tcatgctcgt ctcggtgacg ttctatgtct ggttcatcaa ccgcggccgg cgtggtcgcg
     1321 aagtgagcat gctgtgagcg gcggaccgtt cgatcgagcc acagaaggac gccggtgagc
     1381 gcgcccgcga ttcccgccgc gccgctcgcg gccggccgtg agatgccccg cggtcgccga
     1441 ccggcgcggg atcggtccgg ggtcctcgtc gagatctggc tacgcggcca cacctggctc
     1501 gtctacggct tcctctacct gccgatcctc gtcgtcgtcg tcttcagctt caacgcgagc
     1561 acgcgggtca cgaactggac ggggttcagc ctgcactggt acgagttcgt cctcgccagc
     1621 aaggagatcc agcggaacct catcaacagc ctgatcgtgg gcgtggcgac ggcgaccgtc
     1681 tcgacgatcg tcggcacgat cgcggcgctc gggatccagc gggccccgaa gtggttcaag
     1741 ctcccgttcg acgccctcac gtacgtcagc ctgatcgttc ccgagatcgt catcgccctc
     1801 tcgaccctcg tcttcttcgc ggtgacgatc ggccgcggcg ggctgctctc caacctgacg
     1861 ggcggccccg agatcgggct gggctatcac acgatcgttg gcgccctctc ggtgttcaac
     1921 atcagcctcg tcctgctcct cgtccaggcc cgcctctcga acatggaccg gacccacgtc
     1981 gaggcgtcgt tcgacctgta tggaacgccg tggcggacgt tctggcagat cacgttcccg
     2041 cagctcctgc cggcgatcgt cgcgggctac ctcctgtcgt tcaccttcgc gttcgatgac
     2101 tacatcatca cgtcgttcgt ccacggcacg aaccagacga cgatcccgct gtacgtcttc
     2161 ggcgcgctcc ggaagggggt caacccgtcc acgaacgcgg tggccgcctt gatgttgttc
     2221 gtgaccctcg cgatcctcgt cgtcggccag ttcctgctct cgcgccaggc ccggaagagc
     2281 ggcgcagccg ggccggccgg cgtggccggg atgatcgtcg agcagcaggg cggctgagat
     2341 gacgggcgaa ccgagggatt ccggcgaccc gttcgcgatc gacaacgtcg atgcgtggcg
     2401 cgggccgacc gcctcgcagg ccgccggcct gaccctcgcc gcggcccggg ccatcgtggc
     2461 ggcggtcctg gccgaggcgc ggacgctgcg ggtcgcgctg tcatgcgccg tggtcgactc
     2521 gggcgaccag ctcgtcgcct tcgagcggat ggacggcgcc gacctcctcg ggatcacgct
     2581 cgccaaggac aaggcgttca cggcgctgac gaaccacatg ccgacggccg acctgccgcc
     2641 gatcacccag cccggcaccg agttcttcgg ctaccacacg atcgcgggcg gccggacgat
     2701 catcttcgcg ggcggcatgc cgctcgagcg cggcggcgtc ctcgtcgggg cggtcggggt
     2761 cagcggcggc gacagcgccc aggaccagca ggcggtcgag gccggcgtgc gggcattcgg
     2821 cggatcgtag ccggcggccg tccgctgggc tgatgcgccg aacggatccc gtgtcgaccc
     2881 gcccggccgg atccggccgg gcaacgacgg tccggcggga gaggacactt ctgccacgta
     2941 ccatcgaggg cggttggcgg cgggcagcgc gtctctccgg cgcgcgcgac ataccggaaa
     3001 tcgtcccaat ccacgtcaat caccaccacc aatggtcggt ggcagccgtt cggccgccgc
     3061 ggatgcccgg gcggtccgga gagtgggaca cgcacgacgg cggatcgtgg atgactggat
     3121 cgctagtccg ccgcgagact cgtcgcgtcc accagctggt ccgttcgctc gccgatctcc
     3181 gcgacgagtg gtccgaggat cggctcgacc aggcccggat ccgcgccgat cgcggccgct
     3241 tcgacgtcgc ccgtggcacg ggccaggagg cgcagcaggc gctcgacctc gcgtggcgca
     3301 ccgatcgtcg cctggacggc cttctcggcc tggatggtgt gctggcggag atgcgattcg
     3361 aaccggccga gtcggaagcg gttcggccgg tcgccgtccc agaacggtga cgacaccgcc
     3421 agctcgtcgt cggtcatccc ggcgagccgg gcgacgatcc gatcgcggcc gctgcgatgg
     3481 aacgcccgga tgtcgtcgag cgagcccgcg atggccgccc gccacgacgg ttcgtcaacc
     3541 tcggccaggg caatccaggc ctcgtccgag ccgacccgct cgggctcgcc gatgccagac
     3601 cggcgctggt cgagaccgtt gcggatcgtg acgaggaacc cgaggtcggc ctcgatcaga
     3661 tggtcgagga tcgatcggag gggccattcg ccctcgagcg gggccgtgtc gagagcggga
     3721 tcgcgggcgg cgtctgcccg gtcggcgacc gaccacagct cacgccacgc caccaggtac
     3781 cggccgagga tccggccggc gcgaccgggc gcggcggcga cctcgacgcg gcgtgcggcg
     3841 atctcgatcg cggcgtcggc cacctgctcg gtcgtccgca ggatcccgaa ccgcacgccc
     3901 tcctcgtcgt aagcctccca ggcccagaac gcctcgagcg agcggtcgtc gatcgcgcgc
     3961 aggcggcggt cgaacgattc gatcgcggac aggaagacgg cgagcgccgg ggtgggcgcg
     4021 gcgctgccgc tcatccggcg gggacggcct ccggcagagc gagacccgcc tccgacgcca
     4081 ggagggcggc catgtcggtc cgctcccacg gcag
//
LOCUS       DATAIW010000116         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211012, whole genome shotgun sequence.
ACCESSION   DATAIW010000116 DATAIW010000000
VERSION     DATAIW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211012"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1056)
                     /locus_tag="VHM48_03900"
     CDS             complement(139..1056)
                     /locus_tag="VHM48_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX2754578.1"
                     /translation="MRIATWNVNSLKARTDAVERWLERAQPDVLLMQETKLADGEAPE
                     LPFRMAGYELVHHGEGRWNGVAIASKVGATDVVTNFGDGPVRDSSAGAAIAVGEDDFD
                     PFDEARMVSAICGGVRVVSLYAPNGRVVGSPFYTGKLRWFERLRRWLDETATPDQPLV
                     LGGDLNATPTDDDVWDAVAAHGGTHVSEPERAALASLRDWGLVDAYRAAVPDAPGRFS
                     WWDYRAGMFHRNEGMRIDLLYMTKVVADRIVWAEIDREARKGPPTPSDHAPVVVDLDE
                     RGLAFDAGWEGALSRIQARTKPRAPKIRA"
     gene            1250..3007
                     /locus_tag="VHM48_03905"
     CDS             1250..3007
                     /locus_tag="VHM48_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2754579.1"
                     /translation="MDAPADPAALIERIRASVIGDDEAVAGPFGIRRVTYADYTASGR
                     ALTFVEDYLRDAVLPLYANTHTESSGTGLQTSRFREDARAIIRTAVGGNKDDHAVIFT
                     GSGMTGAINLLVGVLELRIPAGLDERYGLRAHIPANERPVVLIGPYEHHSNELPWRES
                     IAEVITIGEDGDGRIDLEQLRRALEETDGRPLRMGSFSAASNVTGILSDTRAISVLLH
                     RHGALSFWDFAAAGPYVEIEMEPHRPGPAGDGAPDDGDAALDYKDAIVLSPHKFIGGP
                     GTPGVLVARRELFRNRVPTMPGGGTVAYVNPLEHVYLDDPEDREEGGTPAIVESIRAG
                     LVFALKQAVGVAAIRAREEDFIDRAIAAWSAEPGIGILGNPELPRLSIVSFTVRHAGR
                     YLHHNFVVAVLNDLFGIQSRGGCSCAGPYGHRLLGIDIETSHAFEREITRGCEGIKPG
                     WVRVNFNYFISEAVFSYITEAVAMVARDGWRLLPWYRFEPATGMWRHAAGSPEPPLSL
                     HDVSFAADGLSYPTHRHRAGEEALAGHLDEASRILADPVAVLGAAPASEGPPLVGDDF
                     ESLRWFWLPDEVRQAVEAG"
BASE COUNT          429 a         1093 c         1060 g          433 t
ORIGIN      
        1 ccccggatcc gcgatgtggc ctcccaggcg aaggaggtcc acctgatgtt caacaactgc
       61 tacgccaact acggcacgac gaacgcccgc gagatcgcgg acctgctcgc cgaggagatg
      121 gcgaacgcgg gctgagcgtc aggctcggat cttcggcgcg cgcggcttcg tccgagcctg
      181 gatccgcgac agggcgccct cccagccggc gtcgaaggcg agaccgcgct cgtcgaggtc
      241 gacgacgacc ggggcgtggt cagacggggt cggcggcccc ttgcgcgcct cgcggtcgat
      301 ctcggcccag acaatccgat cggcgacgac cttcgtcatg tagagcaagt cgatccgcat
      361 cccctcgttg cgatggaaca tcccggcccg gtagtcccac caggagaagc ggcccggcgc
      421 gtcggggacc gcggcacggt aggcgtcgac gaggccccag tcgcggaggc tggcgagggc
      481 cgcccgttcc ggctcggaca cgtgggtccc gccgtgggcc gcgaccgcgt cccagacgtc
      541 gtcgtcggtc ggggtcgcgt tgagatcgcc gccgaggacg agcggctggt caggcgtggc
      601 ggtttcgtcg agccaccgcc gaagccgctc gaaccagcgc agcttgccgg tgtagaacgg
      661 gctgccgacg acgcggccgt tcggagcgta gaggctcacg acccggacgc cgccgcagat
      721 cgccgacacc atccgggcct cgtcgaacgg gtcgaagtcg tcctcgccga ccgcgatcgc
      781 cgccccggcg ctgctgtcgc ggaccgggcc atcgccgaag ttggtgacga cgtcggtcgc
      841 gccgaccttg ctcgcgatcg cgacgccgtt ccagcgcccc tcgccgtggt ggacgagctc
      901 gtagccggcc atccggaagg gcagctcggg ggcctcgccg tcggccagct tggtttcctg
      961 catgagcagg acgtccggct gggcgcgctc gagccagcgc tcgacggcgt cggtccgggc
     1021 cttcagcgag ttgacgttcc aggtcgcgat ccgcatgccg acaagggtag ggcgaaacgc
     1081 ggtcgctggg cggatcgcgc ttcgtcctgg cctgacccga gtgacgggtc agaatcgctg
     1141 gcgccacgct tgacaccggc cggaacgaca ccgggccacg ctcgctgcac gccgatctga
     1201 cccgagtggc gggtcggcgc aacggggcct gcgacgtacc atggccatcg tggacgcacc
     1261 cgccgatcct gccgccctga tcgagcggat ccgcgcgtcc gtgatcggcg acgacgaggc
     1321 ggtcgccggg ccgttcggga tccgccgggt cacctacgcc gactacaccg cgtcgggccg
     1381 cgccctgaca ttcgtcgagg actacctccg cgacgcggtc ctgccgctct acgccaacac
     1441 ccacacggag tcgtcgggaa ccgggctcca gacgagccgc ttccgcgagg acgcccgggc
     1501 aatcatccgg accgccgtcg gcggcaacaa ggacgaccat gcggtgatct tcaccggctc
     1561 cgggatgacc ggcgcgatca acctcctcgt cggggtgctg gagctgcgga tcccggccgg
     1621 tctcgacgag cgctacgggc tgagggcgca catcccggcg aacgagcggc cggtcgtcct
     1681 catcggcccg tacgagcacc acagcaacga gctgccgtgg cgcgagtcga tcgccgaggt
     1741 gatcacgatc ggcgaggacg gcgacggccg gatcgacctc gagcagctcc gccgggcgct
     1801 cgaggagacc gacggacggc cgctccggat ggggtcgttc tccgcggcct cgaacgtcac
     1861 cggcatcctg tccgacaccc gcgcgatctc ggtcctgctc caccgccacg gcgcgctctc
     1921 gttctgggac ttcgcggccg ccgggccata cgtcgagatc gagatggagc cccaccgacc
     1981 gggtccggcc ggcgacggcg cgcccgacga cggcgacgcc gccctcgact acaaggacgc
     2041 gatcgtcctc agcccccaca agttcatcgg cggccccggc acgccgggcg tcctcgtcgc
     2101 ccgtcgcgag ctgttccgca accgcgtgcc gacgatgccc gggggcggca cggtcgccta
     2161 cgtcaacccg ctcgagcacg tctatctcga cgaccccgag gatcgcgagg agggcgggac
     2221 ccccgcgatc gtcgagtcga tccgggccgg gctcgtgttc gcgctcaagc aggcggtcgg
     2281 ggtcgcggcg atccgggccc gcgaggagga cttcatcgac cgggcgatcg cggcctggtc
     2341 ggccgagccc gggatcggga tcctcggcaa cccggagctg ccgcggctct cgatcgtgtc
     2401 cttcaccgtg cgccatgccg ggcggtatct ccaccacaac ttcgtcgtcg cggtcctcaa
     2461 cgacctgttc gggatccagt cgcgcggcgg ttgctcgtgc gccggcccgt acggccatcg
     2521 actgctcggg atcgacatcg agacctcgca cgccttcgag cgcgagatca cccgcggctg
     2581 cgaggggatc aagcccggct gggtccgggt gaacttcaac tacttcatca gcgaggcggt
     2641 gttctcgtac atcaccgagg ccgtcgcgat ggtcgcgcgc gacggctggc gcctgctgcc
     2701 gtggtatcgc ttcgagccgg cgaccgggat gtggcgtcac gccgcgggat caccggagcc
     2761 gccgttgtcg ctgcacgacg tgtcgttcgc ggcggacggg ctgtcctacc cgacccatcg
     2821 ccaccgggca ggggaagagg cgctggccgg tcatctcgac gaggcatccc ggatcctggc
     2881 cgatccggtc gccgttcttg gggcggcgcc cgcgtccgaa ggcccgccgc tcgtcggcga
     2941 cgacttcgag tcgctccgct ggttctggct ccccgacgag gtccggcagg ctgtcgaggc
     3001 tggctgacgg cccac
//
LOCUS       DATAIW010000117         6404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_121702, whole genome shotgun sequence.
ACCESSION   DATAIW010000117 DATAIW010000000
VERSION     DATAIW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121702"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VHM48_03910"
     CDS             <1..1380
                     /locus_tag="VHM48_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2754580.1"
                     /translation="GVHRRQRPAPLRRRRLLPMAASLVAFGLAVVLFAQGARGSYVPA
                     MVGIPFLGIVLGIAYAGPWVTAAIGAAVVRGARGPSGLLAGRRLLDEPGSSFGAVAGV
                     VLAVFVGSAFFGIASFARAAVPTTDQLGVREGVLVSRYGDADRAEQTATRLRSTVGVS
                     AVAVLREVVVTTPQTVDGSEPDLAVVVDCRSFVDTLAAAGLSCGDGLEHLGPGGRPIT
                     DGNALTLGFLSEPAVLPVGVTRAVHLSIPPDRIDRYVPDGLAASSGPLPAVLIEPTAV
                     GSGIDGLSPTRLLVATDGSPAAIERARTVIESAMPTAVVTTLEEESADASARMLELAR
                     VVSLGVFGAMLLAGASLAIAVATGLVERRVPFALLRLAGMPLARLRLVLFLEAAAPLV
                     AVSAVSAFLGTIVVQVLLRAQSRTAVPPPDLMGVALLGLAIGGALAVVASALPLVGPV
                     TATSETRFE"
     gene            complement(1792..2168)
                     /gene="ssrA"
                     /locus_tag="VHM48_03915"
     tmRNA           complement(1792..2168)
                     /gene="ssrA"
                     /locus_tag="VHM48_03915"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2523..3149
                     /gene="ureA"
                     /locus_tag="VHM48_03920"
     CDS             2523..3149
                     /gene="ureA"
                     /locus_tag="VHM48_03920"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX2754581.1"
                     /translation="MRLTAWEEERLLIFGAAELARRHLSAGIQLNAPEAIAIICDALL
                     EAARAGRSYAEVEAAGLAAVAPDDVIDGVRALVDEVRLEVLVGDGTRLIVLVDPLGQG
                     RPMAPDGPGAVVAGRGSAGGASAERERRRLTVRSESGRTVRVSSHHPFDRVNARLRFD
                     RAAAAGFRLDLPAGVSEAWAPGETKEVDLVRFGGSGGDAAGEGARDGR"
     gene            3146..4840
                     /locus_tag="VHM48_03925"
     CDS             3146..4840
                     /locus_tag="VHM48_03925"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX2754582.1"
                     /translation="MSGGLHPAERLARYGPTAGDRLRLADTDLWVRVTEDRQASGDEP
                     IWGYAKTLRPRMTQTSRASDSELDTVVAGALVIDPTIGIVKADIGIKDGRIVGVGRAG
                     NPAISDGIELEIGPHTAPIMAYGLIATPGAVDSHVHLVSPTLMPAALSGGVTTLITAG
                     FEEPPWAMERTLGAMTEWPLNIGIQACARSEVPADFEALLDAGAVGFKIHEDYGADPA
                     LIDATLRFAEAAGVVVSLHTDGLHETAELEDTIAAIGGRTVHAYHVEGTGGGHVPDLL
                     ALVREPNIICSSTTPTIPFGVSAAAEHVAMIVLNHGGSMAVAADVALAADRIHPATMA
                     AEGPLHDLGAIQIINSDSQGMGRIMETVRRSLQLASVLTRWRGSTLKRAMDVDGADGP
                     DDNERVLRYLAKVTIEPAITHGLADHVGSLRSGRLADIVLWKPAWFGAKPELVLKSGV
                     AAWAPLGEGNATVERAEPTRYRGDWGAGAGLASRLGVTFASAATADAVRRRSVGGREI
                     VPIGRTRGLTRTDLWLNRSTASIEIDRTDGRVTLDGQRLAIEPVDELPLSRRYFLR"
     gene            complement(4864..5946)
                     /locus_tag="VHM48_03930"
     CDS             complement(4864..5946)
                     /locus_tag="VHM48_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX2754583.1"
                     /translation="MNPSLSRPPLRLGPTIRAVDLHACGEPGRVIVGGVEGVPGGSMF
                     EAMTWLRANRDDLRLRMLREPRGFPAANCNLVLPSSHPEAAAGYVIMEQVEYPGMSGT
                     NTMCVVTALLETGMLPMTEPVTELTLEAPAGLIRVRADCAGGKVTGVTFRNVPAFATH
                     LDAIVEVPHLGTVTVDVAYGGMFYVIASAESFGLRLTPDEGADIVRITEMIKAAANDQ
                     LPVVHPEQPGFAGITIGQLSGPAHDPANSMRNVVTVSTGALDWDRHATWTGAIDRSPC
                     GTGTSARMATLHARGRLAIGEDFRHEGILGTVFTGRLLEETAVGERAAVVPQITGQAW
                     ITGIADYVVDPTDPFPDGFTIGDIWA"
     gene            complement(5943..>6404)
                     /locus_tag="VHM48_03935"
     CDS             complement(5943..>6404)
                     /locus_tag="VHM48_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754584.1"
                     /translation="VDDAASVRDVAEAAWRDTYAGLLSASTIETFIDGAYSIDRLQRR
                     IDRHTFLVVEEEGSITAFADAVIEADRLNLVAIYALPDRRGRGAGTGLLEALRARFPG
                     LPVTADVLVGNRKGEVFYERRGFVPREEIEADLFGEPAVERRWWLDARLDP"
BASE COUNT          898 a         2272 c         2263 g          971 t
ORIGIN      
        1 ggcgtccacc gccggcagcg gcccgccccg ctccggcgtc ggcgcctgct gcccatggcc
       61 gcgagcctcg tggcgttcgg gctggccgtc gtgctcttcg cacagggggc tcgcggctcg
      121 tacgtgccgg cgatggtggg catccccttc ctggggatcg tcctcggcat cgcctacgcc
      181 gggccctggg taaccgcggc gatcggagca gcagtcgtgc gcggagcacg tgggccgtca
      241 ggcctacttg ccgggcgccg gctgctcgac gagcccgggt cctcgttcgg cgccgtcgcg
      301 ggcgtcgtgc tcgccgtgtt cgtggggagc gccttcttcg gcatcgcgtc gtttgcgcgg
      361 gccgcggtgc cgacgaccga ccagctcgga gtccgcgagg gggtgctcgt ctcgcggtac
      421 ggcgatgcag acagggccga gcagacggcc acgaggctgc gctccaccgt cggggtgtcg
      481 gccgtggccg tcctgcgcga agtcgtggtc acgaccccgc agacggtcga cggcagcgag
      541 ccggacctgg ccgtggtcgt cgactgccgc tcgttcgtgg acaccctggc cgcggcagga
      601 ctgtcctgcg gggacgggct ggagcacctg gggcccggcg gacggcccat caccgacggc
      661 aacgcgctga ccctcggttt cctctccgag cctgccgtcc tcccggtggg ggtgacccgc
      721 gcggtgcacc tgtcgatccc gcccgaccgc atagaccggt acgtcccgga cggtctggcg
      781 gcatcgagcg ggcccctgcc cgccgtcctg atcgagccga ccgctgtcgg ctcgggcatc
      841 gacggactca gtcccaccag gctcctcgtg gccacggacg gctcccccgc cgcgatcgag
      901 cgtgcgcgga cggtcatcga gtcggcgatg cccacggcgg tggtcaccac gctcgaggaa
      961 gagtcagcag atgcctccgc gcggatgctg gagttggcac gggtggtctc gctcggcgtc
     1021 ttcggggcga tgctgctggc cggtgcgagc cttgcgatcg ccgtcgcgac tgggcttgtc
     1081 gagaggcgtg tgccgttcgc gttgctccgc cttgccggga tgccgctcgc gcggctgcgg
     1141 ctcgtcctgt tcctcgaggc cgcggcgcct ctcgtcgcgg tgtctgcagt ctcggccttc
     1201 ctcgggacga tcgtcgttca ggtgctgctt cgggctcagt ccaggacagc ggtcccgccg
     1261 ccggacctga tgggtgtggc gctccttggg ctggcgatag gtggcgcgct cgcggtggtg
     1321 gcatcggcgc tgccactcgt cgggccagtc acggcgactt cggagacgcg tttcgagtag
     1381 gaccaccccg atccaaggtg tcccgccgca ctcaaatcgc cgtcaagcgg tcttggccgg
     1441 gccgttcctc cgcgtttcgt cgccacggag ctgcgcaaca cgctgcgcct gcagcgcctc
     1501 gatcgcgcca tcgcggacaa cttgtgcctt cggtcacgct tagtcgcgcg gccgtcgcta
     1561 ccttttgtgc cgaccctcgg cacaagaggt atacgttctg gcacgtccct cgcacaaaac
     1621 gtatgggcgt cggtccggtt ccgaccgtca aacgcgaatg acacacagga tttgccagtc
     1681 tcgccagagg ctgcagtctc agtttggcca ctgccaaatg cactctcagt ttggccacaa
     1741 tggccaaatc caaagtcctc tgacaaggag cagagggagt cgcgatcgag ttggtggaga
     1801 tgggggagag tcgaactccc cgtccagaac ccttcgccgg agaccactac gagcgtgtcc
     1861 gatggtttgt cgtcgaccgc ccggacgggc atcggcactc tgccggacgg tccagtcacg
     1921 tgtccctaga tcgggcttga ttccggacta cgcgacgctg atccggaccg cagccccgct
     1981 gaatgacgct tccaccaccc gcgggaccga ggtggcttcc acgctcacac tagctgctta
     2041 cgcagcgagg gcgagagtcg actggcggtt gccaggtact tcgttttgct gcctgattaa
     2101 cgaggcctga gcagcatcct cggctcgcgt tccccggtag ccaagccctg tcgaatccac
     2161 acatccccac aggaccgaac gaccgttcgg atcgcggatt gtagcccgtc ccgcccgatc
     2221 ggccaagcgc ggcggccgcg gcggcatcga cccggacgat ccgctcagcg cgccgcctca
     2281 gcgcgccgcc tcatcgcgcc gccgacgggc tgcctgccag gcggtgatcg ccggtcccca
     2341 ccagactgac gccgcgaggt gacgaacgcc atcgggtgcc ccgtcggcag gcacggcgga
     2401 cgatccgcgc cacgtgtgcc ggaaccgctc ccccccgggt caggccgcgg cggcgcgtca
     2461 gccgccgtcg acgagcctga ccaggagcgg caggacctcg acgccctcgc tacgatgcgc
     2521 cgatgcgact gacagcgtgg gaggaggagc ggctcctgat cttcggcgcg gccgagctgg
     2581 ctcggcgtca cctgtccgcg gggatccagc tgaacgcgcc cgaagcgatt gcgatcatct
     2641 gcgatgcgct gctcgaggcg gcccgcgccg gccggtcgta tgcggaggtc gaggccgcgg
     2701 gtctggcggc cgtcgcgccc gacgacgtga tcgacggcgt ccgcgccctc gtcgacgagg
     2761 tccgcctcga ggtgcttgtc ggcgacggaa cgcgcctgat cgtgctcgtc gatccgctcg
     2821 gccagggccg gccgatggcg ccggacgggc ccggggccgt cgtcgccggt cgcggatcgg
     2881 cgggtggggc gtccgcggag cgcgagcgcc gccggctgac cgtccgcagc gagtccggtc
     2941 ggacggtccg cgtctcgtcc caccatccct tcgatcgggt caacgcgcgt ctccggttcg
     3001 atcgcgccgc ggcggccggc ttccggctcg acctgccggc cggcgtgtcg gaggcatggg
     3061 cacccggcga gacgaaggag gtcgacctgg tccggttcgg aggatcgggc ggcgacgccg
     3121 cgggcgaagg cgcgagggac ggccgatgag tggcgggctc catcccgcgg agcggcttgc
     3181 ccgctacggg ccgaccgccg gcgatcgcct ccgcctcgcc gacaccgacc tctgggttcg
     3241 cgtcaccgag gaccgccagg cgtccggcga cgagccgatc tggggctacg ccaagacgct
     3301 ccggccgcgg atgacccaga ccagccgcgc ctcggactcc gagctggaca ccgtggtcgc
     3361 gggcgcgctc gtcatcgatc cgacgatcgg tatcgtcaag gccgacatcg ggatcaagga
     3421 cggccggatc gtcggggtcg ggcgggccgg caacccggcg atcagcgacg ggatcgagct
     3481 cgagatcggc ccgcacaccg cgccgatcat ggcgtacggc ctgatcgcga cgccgggcgc
     3541 ggtcgacagc cacgtccacc tggtcagccc gaccctgatg cccgcggcgc tgtcgggtgg
     3601 ggtcacgacg ctgatcaccg cggggttcga ggagccgccg tgggcgatgg aacggaccct
     3661 cggggcgatg acggagtggc ccctgaacat cgggattcag gcatgcgccc ggtcggaggt
     3721 gccggccgac ttcgaggcgc tgctggatgc gggcgcggtg gggttcaaga tccacgagga
     3781 ctacggcgcg gatccggccc tgatcgacgc gaccctgcgg ttcgccgaag cggcaggggt
     3841 cgtcgtgtcg ctccacaccg acggcctgca cgagaccgcg gagctcgagg acacgatcgc
     3901 cgcgatcggc ggtcggacgg tccacgccta ccacgtcgag gggaccggtg gcggccacgt
     3961 gccggacctc ctcgccctgg ttcgcgagcc gaacatcatc tgctcgtcga cgacaccgac
     4021 gatcccgttc ggggtgagcg ccgccgcgga gcatgtcgcg atgatcgtgc tcaaccacgg
     4081 cgggtcgatg gccgtcgcgg ccgatgtcgc gctcgccgcg gaccggatcc accccgcgac
     4141 gatggcggcc gagggtccgc tccatgacct gggggcgatc cagatcatca actccgattc
     4201 gcagggcatg ggccggatca tggagaccgt ccgccgatcg ttgcagcttg ccagcgtgct
     4261 gacacgctgg cgtggtagca cgctgaaacg ggcgatggac gtggacgggg ccgacggacc
     4321 cgatgacaac gagcgcgtcc tgcgctacct cgccaaggtc acgatcgagc ccgcgatcac
     4381 ccacgggctc gccgatcacg tcggctcgct ccggtcgggc cgcctcgccg acatcgtcct
     4441 ctggaagccc gcctggttcg gcgccaagcc ggagctcgtc ctgaagtccg gggtcgcagc
     4501 ctgggcaccg ctcggcgaag gcaacgccac cgtcgaacgg gccgagccga cgcgctaccg
     4561 gggagactgg ggcgcgggcg ccggtctcgc atcgcgtctc ggtgtcacgt tcgcgtcggc
     4621 tgcgactgcc gacgccgtgc gccgacgctc ggtcggcggc cgcgagatcg tcccgatcgg
     4681 gcgcacccgc ggcctgaccc gaacggacct ctggctgaac cgatcgaccg cgtcgatcga
     4741 gatcgatcgg accgacggcc gggtgaccct cgatggccaa cggctcgcga tcgagccggt
     4801 cgacgagctg cccctgagcc ggcgctactt cctgcgttga tcagacgtgc ccgcccgccc
     4861 ggctcacgcc cagatgtcgc cgatcgtgaa cccgtcgggg aaggggtcag tcggatcgac
     4921 gacgtagtcc gcgatcccgg tgatccacgc ctgacccgtg atctgaggca cgaccgccgc
     4981 ccgttcgccg accgccgttt cctcgagcag gcgtccggtg aacaccgtcc cgaggatccc
     5041 ctcgtggcgg aagtcctcgc cgatcgcgag gcggccgcga gcatggagcg tcgccatcct
     5101 tgccgacgtc ccggtcccgc acggcgagcg gtcgatcgcc cccgtccacg tcgcatggcg
     5161 gtcccagtcc agggcgccgg tcgagacggt cacgacattc cgcatcgagt tcgccggatc
     5221 gtgggccggc ccggacagct ggccgatcgt gatcccggcg aaccccggct gctcggggtg
     5281 gacgaccggc agctggtcgt tcgcggcggc cttgatcatc tcggtgatcc ggacgatgtc
     5341 ggcgccctcg tcgggcgtca gacgcagccc gaacgactcg gccgacgcga tgacgtagaa
     5401 catcccgccg taggcgacat cgaccgtcac cgtgcccagg tgtgggacct cgacgatcgc
     5461 gtcgagatgg gtcgcgaagg cgggcacgtt ccggaaggtc acgcccgtca ccttgccgcc
     5521 ggcgcagtcc gcccggacgc ggatcaggcc ggccggcgcc tcgagcgtga gctcggtgac
     5581 gggctcggtc atcggcagca tcccggtctc gaggagggcc gtgacgacgc acatcgtgtt
     5641 cgtcccgctc atccccgggt attcgacctg ctccatgatc acgtagccgg cggccgcctc
     5701 cgggtgcgac gacggcagga ccaggttgca gttcgcggcc gggaagccgc gcggctcgcg
     5761 gagcatccgt agacggagat catcgcggtt ggcccgcagc caggtcatcg cctcgaacat
     5821 cgagccgccg ggcacgcctt cgaccccacc gacgattacc cggccgggct cgccacaggc
     5881 gtggaggtcg accgcccgga tcgtcggacc gagacggagc ggtggccggg agagggaagg
     5941 gttcatggat cgagcctggc gtcgagccac caccgacgct cgaccgctgg ctcgccgaac
     6001 agatccgcct cgatctcctc gcgtgggacg aacccgcggc gctcgtagaa cacctcgccc
     6061 ttgcggttgc cgacgagcac atcggccgtc acgggcagac ctgggaacct ggcgcggagc
     6121 gcctccagga gccccgtgcc ggcgccccgg ccgcggcgat ccggaagggc gtagatggcg
     6181 acgaggttca gccgatctgc ttcgatgacg gcgtcggcga acgccgtgat cgagccctct
     6241 tcttcgacga cgagaaacgt gtgcctgtcg atccggcgct gaagccgatc gatcgagtag
     6301 gcgccgtcga tgaaggtctc gattgtcgaa gccgacagca agccggcata ggtgtcacgc
     6361 catgccgctt cagccacgtc tcgcacggag gcggcgtcgt ccac
//
LOCUS       DATAIW010000118         9108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_65380, whole genome shotgun sequence.
ACCESSION   DATAIW010000118 DATAIW010000000
VERSION     DATAIW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65380"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..721
                     /locus_tag="VHM48_03940"
     CDS             347..721
                     /locus_tag="VHM48_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754585.1"
                     /translation="MDSGDLLRQLVYLPQASIFIAEARREVVGGAVLALRPSVRAGGF
                     VGAVDLIVVDPDHDADRVTDALLEEILRSARNKGCSVVEAALSDDPAERTRLERHGFV
                     EAGPRAECRVATVGAAGRRSRN"
     gene            788..2827
                     /locus_tag="VHM48_03945"
     CDS             788..2827
                     /locus_tag="VHM48_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2754586.1"
                     /translation="MGKNVAVFVDVANIFYAAKAAGTDIDYVTLLKAATAGRDFVRAY
                     AYTGLDPDNENQRNFHAFLARNHYKVVSKDIRKYGDGKVKANLDIELVVDLMKTARNL
                     DIAIVISGDGDFAPAIRAVQEMGVRVEVISFRGNTSSDLIEVADLFTDITQLARVDKA
                     SSRSGRRVAGDDEDLSMTEVPDKQTEGTGQRGRGRGRGRGRPGEAEPVAARGRTGGRS
                     SVSRVAASSPEPVEAPAAAGNGALTVLPGEKLSRVAAAASAPVAGPSGDLDDEEITPF
                     DIDPESMPGGVAEGAEGDAEADGEGEAGRRRRRRRGGRGRGRGRGRDGEALPAGETAT
                     PDGEALDDEEDDEPDVAPAPRGPRTTPFGAVWDSQLGTPTSAPASLGPVNDDEDFDEP
                     EIPEYLIAEQRRGRQGQGQGGQGSRGGQGRGGPRGGRSSYSSAIDRERYGRGGGGGIN
                     RYPDVSGRTGQGGRPSGQPGGSYARQDASYSRPQSAERFDRPAPRSGDPWSEVPPELE
                     EMLRAQLAQSPKARRPERSPAASRIAAAEAAGEIETMTETSADTALETAAPKRRTSSR
                     RKATGEATATAASLSTPTADAATDAVAAEPAAPAAKRRTATRKKVDAAAAPAVESAPE
                     AVATAPKRRPAPRKKADAAESPAPATAAGGDADATPAESAGPKRRTTSRKKADPA"
     gene            2970..4256
                     /locus_tag="VHM48_03950"
     CDS             2970..4256
                     /locus_tag="VHM48_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754587.1"
                     /translation="MASIRGMVAGRVPHAILISGPSGIGKTTLALDLAAGLLCDDPDP
                     AARPCRACRACRLVERGRHPDVHRLAPAGAGDQIRIGTRDRPEDGTVRRLASDLVLMS
                     VEGRARIAIIERADRLTDDAQTALLKTLEEPPAGVTIILCADDEDRLLPTVRSRSARI
                     RLGPVATREIEAILSDAGVAEPPLAARLARIAAGRPGAARALALAPEAVAARDEIART
                     LLDLLTATPAPRLAAIKDLTNLASALARSLDRAAAEDDGTDRPATGPVKRGRTKAKGP
                     AANLGQPGATGATGPAGDDETGDEDAEASADPGAAKTSVPAAERRRAASILVGLWREL
                     ARDLLVVVLGEERQVRDQAFLDDLREAAAKLIQPAAHDGSSTAFPAGSVEARGRTALG
                     SFLGRLDAAGELLEANVRPELVLDSLLLHWPRAAAT"
     gene            4253..4570
                     /locus_tag="VHM48_03955"
     CDS             4253..4570
                     /locus_tag="VHM48_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2754588.1"
                     /translation="MTDDEGAAPGGRLEATVRGRVQGVGFRYFVVRRARELGVDGWVA
                     NSADGSVRCVAEGSPDALDALEGTLRTGPLGAVVDAVDVVRMTATGRLERFTIRSGAH
                     SGD"
     gene            complement(4778..5071)
                     /locus_tag="VHM48_03960"
     CDS             complement(4778..5071)
                     /locus_tag="VHM48_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754589.1"
                     /translation="MNATVKDDVDPRSANGDRSPSERPSPRTLAAREAAIGAGEKTEE
                     LTVSGLVPRLRIRPGRLGADPAGIAARADTLEVGDAIEDRPVEIGQVFGKRGD"
     gene            5115..5420
                     /locus_tag="VHM48_03965"
     CDS             5115..5420
                     /locus_tag="VHM48_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754590.1"
                     /translation="MDSIDTIGDAIDAARPAYDELTALAEDVEDEWSYINDLASAWVE
                     RLDEVADARGHDPAPAGAAEAVTVLAAEVAAVDDPHRAIDWLSTFPQVLLIALGERP"
     gene            5417..6106
                     /locus_tag="VHM48_03970"
     CDS             5417..6106
                     /locus_tag="VHM48_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754591.1"
                     /translation="MRFQDASPDARAVVYAGIQADPLVARAATLLADATQPQRILARA
                     VMNGMSTDPTVWQAMFPTLFGAPVPADGPERARSEAILAASLAVAERGEQVRSQFRGA
                     IVESLAELLVRRRPGLASALPVTVRRERRILFDGVRAEIHPYDVTVEASGAAEAIDCK
                     WGARGINADVLHQLDDARTHAADEDETLTATIVVFDAERSCVVRLERQTAPHAATRLI
                     AIETLDRLAGR"
     gene            6103..6930
                     /locus_tag="VHM48_03975"
     CDS             6103..6930
                     /locus_tag="VHM48_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEX2754592.1"
                     /translation="MTTTERPGLLDDGRSTWQTTYRVRFDEAGPDGVLRTSGFMRYAQ
                     DLAWQHSADLGFGRAWYAERGLTWLVRAAELVITAAPEMGSTITARTAIVGIRRVFAR
                     RRGEFRLADGTPAGWVHTDWVLIDARGALTRIPSIFPEAFGGTEVMGAVGRIPLPPTS
                     REAVHRRFAVRPHELDPMDHANNAVYLDWLEEAILDAAGDRDGRAAARDLPRRYRMEF
                     ALAVEAGAELDGAAWRDDDGWSYRLAGADGGADRFRARMATGPAAVAAAANDTEEAS"
     gene            6927..8129
                     /locus_tag="VHM48_03980"
     CDS             6927..8129
                     /locus_tag="VHM48_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2754593.1"
                     /translation="MTRTVLTGGRVFDGTGSDPALADVAIEDGRIVGLGTGLDGDAEV
                     DVRDRTVLPGMFDCHTHVCISNVDLWGHAQQPFSRQFYEAARNLQKTLAIGITSVRDA
                     GGADLGIKEAVADGTIAGPRLQISIVMLSQTGGHGDDWYPSGAEVPFMGPHPGRPSGI
                     VDGVDEMRRKVRELHRAGADVIKVATSGGVLSARDDPRHAHFRPAELDALVEEATAAG
                     MFVMAHAQGADGIKNAVRAGIRSIDHGIYLDDEAIELMLRRGTWFVPTLVAPQGVLDA
                     VDAGVSLPAAVVDKARMVIDIHRAAFRRAVEAGVRIAMGTDSGVTPHGRNLRELQLMA
                     DGGMPPAAVLEATTRSAAELMGVDDDLGTIAEGKLADLVVVARDPYDFTDLGDRVEQV
                     WQGGKRVV"
     gene            8311..8511
                     /locus_tag="VHM48_03985"
     CDS             8311..8511
                     /locus_tag="VHM48_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754594.1"
                     /translation="MFRRADPLRIQAARRAAAIARLISAGELPDRAAVAVARWEATID
                     GPADRTDWEAFEGWLAGRRPRT"
     gene            complement(8544..>9108)
                     /locus_tag="VHM48_03990"
     CDS             complement(8544..>9108)
                     /locus_tag="VHM48_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754595.1"
                     /translation="ASESRIREQKLAGDQDSLRREEQLELARIARQRREQTENLATQR
                     QALELEQERLHAQLAVDADRVNAEAPVRLLRIEKERGILAEELELRHLQNQVKTLDVE
                     RDLLLPRAQQDLRREMLPLEQAPQMVGAASRVLQGTNLSIYGENSQLVGQLAPIFELL
                     TRAVTRATQAAPERAQEPQPEAAGAEV"
BASE COUNT         1262 a         3313 c         3313 g         1220 t
ORIGIN      
        1 gtacgtgacg gacgacgacg ctggacgctg gctcggacgg cgcgcaggtt gccgccgaag
       61 cgatgccgta tcataggcgc cagcggaccg aatgcaacaa cggtcggcgc gacccgggcg
      121 tgtgctgtcg agccttctgg ctcgccctcc cgcgaggtcc cgtcgacgac ggagcgaacg
      181 ggtccgctcc cgatcggcga aggacgcgaa tcccattgct cccgccgtcc tcacgaggtg
      241 ctccgcccgc gtggaatacc tgatccgccc ggctcgcatc acggacatcg aacggcttgt
      301 cgcgctcagc ggcggttcgg ttcggtcgcc cgggcctgcg ggatcgatgg attctggaga
      361 cctgctccga cagctggtct atctcccgca ggccagcatc ttcatcgccg aggctcggcg
      421 cgaagtggtc ggaggcgcgg tcctcgccct ccgtccgtcc gtccgggccg gtggtttcgt
      481 cggtgccgtg gacctcatcg tggtcgatcc tgaccatgac gcggaccgcg tgaccgacgc
      541 gctgctcgag gagatcctcc gatcggcgcg caacaagggc tgcagcgtcg tcgaggcagc
      601 cctctcggac gatccggcgg agcggacccg cctggaacgt catggtttcg tcgaagccgg
      661 tccgcgtgct gagtgccggg tggccacggt cggggccgcc gggagacgca gcaggaactg
      721 acccgggacc gtcaggcagc gcgatcgcgc cgacggtcct cgaccgacaa cattctgagg
      781 agtcaaggtg ggcaagaacg tcgccgtctt cgtggacgtg gcgaacattt tctacgcggc
      841 caaggcggcc ggtacggaca tcgactacgt cacgctgctc aaggcggcca ctgccggacg
      901 cgatttcgtc cgtgcgtatg cgtacacggg gctcgacccc gacaacgaga accagcgcaa
      961 cttccacgcg ttcctcgcgc ggaaccacta caaggtcgtc agcaaggaca tccgcaagta
     1021 cggcgacggc aaggtcaagg cgaacctcga catcgagctc gtcgtcgacc tgatgaagac
     1081 cgcccgcaac ctcgacatcg cgatcgtgat ctcgggcgac ggcgacttcg ctccggcgat
     1141 ccgggcggtc caggagatgg gcgtccgcgt cgaggtgatc agcttccgcg gcaacacctc
     1201 gagcgacctg atcgaggtcg ccgacctgtt caccgacatc acccagctgg cccgcgtcga
     1261 caaggcatcc tcgcgctcgg gccggcgggt cgccggcgac gacgaggacc tgtcgatgac
     1321 cgaggtcccc gacaagcaga ccgagggcac cggccagcgc gggcgtggcc gcggccgtgg
     1381 tcgcggtcgg ccaggcgagg ccgagccggt tgcggcccgt ggccggaccg gcggccgatc
     1441 gagcgtctcc cgggtcgccg catcgtcccc cgagccggtc gaggcgccgg cagccgcggg
     1501 caacggcgcg ctgaccgtcc tgcccggcga gaagctctcc cgcgtcgctg cagccgcatc
     1561 cgcccccgtc gccggcccgt caggcgacct cgatgacgag gagatcaccc cgttcgacat
     1621 cgaccccgaa tccatgcccg gcggcgtcgc cgaaggcgcc gaaggcgacg cggaagcgga
     1681 cggtgagggc gaagccggcc gtcgccgccg ccgccgacgc ggtggtcgcg gtcgcggtcg
     1741 cggtcgcgga cgagacggcg aggccctgcc ggccggtgag accgcgacac ccgatggcga
     1801 ggcactcgac gacgaagagg acgacgagcc cgatgtggcc ccggccccgc gtggcccgcg
     1861 tacgacgccg ttcggcgcgg tctgggactc gcagctcggc acgccgacct cggcgccggc
     1921 gtcgctcgga ccggtcaacg acgacgagga cttcgacgag cccgagatcc ccgaatacct
     1981 gatcgccgaa cagcggcgcg gtcgacaggg ccagggccag ggcggacaag gtagccgtgg
     2041 cggccagggt cgtggcggcc cccgcggtgg ccgcagttca tacagctccg cgatcgatcg
     2101 cgaacgctat ggacgtggcg gcggtggcgg catcaatcgc tatccggacg tcagcggtcg
     2161 gaccggccag ggtgggcgac ccagcggcca gccgggcggg tcgtatgccc gccaggatgc
     2221 gagctattca cggccccaga gcgccgagcg cttcgatcgg ccggcccccc ggtcgggcga
     2281 tccatggagc gaggttccgc cggagctcga ggagatgctg cgagctcagc ttgcccagtc
     2341 gccgaaggcc cgccgaccgg aacggagccc ggccgcgtcc cggatcgcgg cggccgaggc
     2401 ggccggcgag atcgagacga tgaccgagac gtccgccgac accgcgctcg agacggcggc
     2461 gccgaagcgc cggacgtcga gccggcggaa ggccactggc gaggcaacag cgacggccgc
     2521 aagcctctcg acgccgacgg ctgatgccgc gactgacgcg gtcgcggctg agccggccgc
     2581 gccggcagcg aagcgacgga cggcgacccg caagaaggtt gacgccgccg cggcgccggc
     2641 cgtcgagtcg gcaccggagg ccgtggccac cgcgccgaag cgccggccgg ccccccgcaa
     2701 gaaggcggat gccgcggaat cccctgcgcc ggcgacggcc gcaggcggcg acgcggacgc
     2761 gacgcccgcc gagtcggccg gcccgaagcg gcggacgacc agccgcaaga aggccgaccc
     2821 ggcctgatcc gcggttggtc ggcccgaccg aaccgagcga cgatttcgtg acccggacgg
     2881 cgagggccga cccggtcggt gccgctgctc cgggcagcag catggcagcc gccgtcggtc
     2941 cgatgcggac tcgcggtcat gaccgggccg tggcgagcat ccggggcatg gtcgccggtc
     3001 gcgtcccgca cgcgatcctg atcagcggac cgtccgggat cggcaagacg accctcgccc
     3061 tcgaccttgc cgccggcctg ctctgcgacg atccggaccc ggcggccagg ccctgccgcg
     3121 cctgccgcgc ctgccgtctg gtcgagcgtg gccggcaccc cgacgtccac cgcctcgccc
     3181 cggcgggcgc cggcgaccag atccggatcg ggacacgtga tcggcccgag gacgggacgg
     3241 ttcggcgcct ggcgtcggat ctcgtcctca tgtccgtcga gggccgggcg cggatcgcga
     3301 tcatcgagcg ggccgaccgc ctgaccgacg acgcccagac cgccctgctc aagaccctcg
     3361 aggagccgcc ggcgggggtc acgatcatcc tctgcgccga cgatgaggat cggttgctgc
     3421 cgaccgtccg gtcccgctcg gcgcgaatcc ggctcggccc ggtcgcgacg cgcgagatcg
     3481 aggcgatcct ctccgatgcc ggcgtcgccg agcctccgct ggctgcgcgc ctcgcgcgga
     3541 tcgccgccgg ccggcccgga gccgcacgag cgctcgccct tgcacccgag gccgtggcgg
     3601 cccgcgacga gattgcccgg acgctgctgg acctgttgac ggccacgccc gcgccgcgac
     3661 tcgcggccat caaggacctc acgaacctgg catcggccct cgctcgctcg ctcgatcggg
     3721 cggcggccga ggacgacggc acggatcggc ccgcgaccgg gccggtgaag cgcggccgca
     3781 ccaaggcgaa gggtccagcc gcaaacctcg ggcagcccgg cgccaccggc gcgaccggtc
     3841 ccgctggcga cgacgagacc ggagatgagg acgccgaggc gagcgccgat ccgggcgcgg
     3901 cgaagacgtc cgttcccgcc gccgagcgtc ggagggcggc gtccattctg gtgggactgt
     3961 ggcgggagct cgcccgcgac ctgctcgtcg tggtcctcgg cgaggagcgc caggtccgcg
     4021 accaggcgtt cctcgacgac ctgcgcgagg ccgccgcgaa gctgatccaa cccgccgcgc
     4081 acgacggatc atcgaccgcg ttccccgccg gatccgtcga ggcgcgaggc aggaccgcac
     4141 tcgggagctt cctcggccgg ctcgatgctg ccggcgagct gctcgaggcg aatgtccgac
     4201 ccgagctcgt cctcgactcg ctcctgctcc actggccgcg ggccgccgcg acatgaccga
     4261 cgacgaaggc gcagccccgg gcgggcgcct ggaggcgacg gtccgcggtc gggtccaggg
     4321 cgtcgggttt cgctacttcg ttgtccgccg ggcgcgtgag ctgggtgtcg acggctgggt
     4381 cgcgaactcc gccgacggaa gcgtccggtg cgtcgcggag ggttcgccgg atgccctcga
     4441 cgccctcgaa gggacgctcc gcactggtcc gctcggtgcc gtcgtcgacg ccgtggacgt
     4501 ggtccggatg accgccactg gccggctcga gcgcttcacg atccggtccg gcgcccacag
     4561 cggcgactga tcgcctgacc tcgcgtcgct gccctcaggg cgaccggttc acgacggttc
     4621 ggggccatcc ggtgcgtgcg ggaaccgaac cgcggtcatt cgggactttc gcctccgcgg
     4681 accgggacga gtgacccgtt cccgccgatc cgcgggtggc ccatcgtcgc gggtgatgga
     4741 gtcccggccc gccccagccg tttccgagcc cgtcgagtca gtcaccacgc ttgccgaaga
     4801 cctgcccgat ctctaccggg cgatcctcga tcgcgtcgcc gacctcgagc gtgtcggcgc
     4861 gcgccgcgat gccggccgga tccgcgccga ggcgacccgg gcgtattcgg aggcgtggga
     4921 ccaggccgct caccgtcagc tcctcggtct tctcgcccgc gccgatcgcg gcctctcgag
     4981 cggcgagcgt tcgcggggat ggtcgctccg acggagatcg gtcgccgttc gctgaccgcg
     5041 ggtcgacatc gtccttcacg gtcgcgttca cgtccgagcc gcggtcgccg cgccggccgc
     5101 gctagtcttg gcgggtggac tccatcgaca cgatcggcga tgcgatcgac gctgcccgcc
     5161 ccgcctatga cgagctgacg gccctggccg aggacgtcga ggacgagtgg tcgtacatca
     5221 acgacctggc gtcggcctgg gtcgagcggc tggacgaggt cgccgacgct cgcgggcacg
     5281 atcccgcgcc ggccggtgcc gcggaggccg tcaccgtcct cgccgcggag gtcgccgcgg
     5341 tcgacgaccc gcaccgggcg atcgactggc tctcgacatt tccccaggtc ctgctgatcg
     5401 ccctcggcga gcgaccgtga ggttccagga cgcgtccccg gacgcccggg cggtcgtcta
     5461 cgccgggatc caggcggatc ccctcgtcgc gcgggcggcc accttgctcg cggacgccac
     5521 ccagccacaa cgcatcctcg cccgcgccgt gatgaacggg atgtcgaccg acccgaccgt
     5581 ctggcaggcg atgttcccga cgttgttcgg cgcgccggtc ccggccgacg gcccggagcg
     5641 ggcgaggagc gaggcgatcc tcgccgcgtc gctcgcggtc gccgagcgcg gcgagcaggt
     5701 ccgctcgcag ttccgcggcg cgatcgtcga gtcgctggcg gagctgctcg tccggcgccg
     5761 gcccggtctc gcctcggctc tgccggtcac ggtccggcgc gagcggcgga tcctcttcga
     5821 cggtgtccga gcggagatcc acccgtatga cgtcacggtc gaggcgtcgg gcgcggcgga
     5881 ggcgatcgac tgcaagtggg gcgcgcgggg gatcaatgcc gacgtcctcc accagctcga
     5941 tgatgcgcgg acgcacgccg ccgacgagga cgaaacgctc accgcgacga tcgtcgtctt
     6001 cgatgcggag cgatcgtgcg tggtccgcct cgagcgccag accgcgcctc atgccgcgac
     6061 ccgcctgatc gccatcgaga ccctcgaccg gctggccggc cggtgacgac gaccgagcgg
     6121 cccggcctcc tcgacgacgg ccgatccacc tggcagacga cctatcgggt ccgcttcgac
     6181 gaggcggggc ccgacggtgt cctgcggacg tcggggttca tgcgctatgc ccaggacctc
     6241 gcctggcagc actcggccga tctcgggttc gggcgggcgt ggtatgcgga gcgcggcctg
     6301 acctggctcg tccgcgccgc ggagctcgtg atcaccgccg cgccggagat gggctcgacg
     6361 atcaccgccc ggaccgcgat cgtcggcatc cgccgggtct tcgcccggcg gcgcggcgag
     6421 ttccgcctgg cggacgggac gccggcaggc tgggtccaca ccgactgggt cctgatcgac
     6481 gcccgcggcg ccctgacccg gatcccctcg atcttcccgg aggcgttcgg gggcacggag
     6541 gtgatgggcg cggtcggccg gatcccgctc ccgccgacgt cgcgcgaagc cgtccatcgc
     6601 cggttcgccg tccggccgca cgagctcgat ccgatggacc acgcgaacaa cgcggtctat
     6661 ctcgactggc tcgaggaggc catcctcgac gccgccggcg accgcgacgg ccgggccgcg
     6721 gccagggacc tgccccggcg gtaccggatg gagttcgccc tcgccgtcga ggccggcgcc
     6781 gagctcgacg gcgcagcctg gcgcgacgac gatggctgga gctatcgcct ggccggcgcg
     6841 gacggcggcg cggatcgctt ccgggcgcgg atggccacgg gcccggcggc tgtcgccgct
     6901 gcggcgaatg acacggagga agcctcatga cccgaacggt gctcaccggc ggccgcgtct
     6961 tcgacgggac cggcagcgat cccgcgctcg ctgacgtcgc gatcgaggac ggacggatcg
     7021 tcgggctcgg gaccgggctc gacggagacg ccgaggtcga cgtccgcgac cggacggtcc
     7081 tgcccgggat gttcgactgc cacacccacg tctgcatctc gaacgtcgac ctgtggggcc
     7141 atgcccagca gccgttctcg cgccagttct acgaggccgc gaggaacctc cagaagaccc
     7201 tcgcgatcgg gatcacctcg gtccgtgacg ccggcggggc cgatctcggg atcaaggagg
     7261 cggtcgccga cgggacgatc gcgggaccgc gcctccagat ctcgatcgtc atgctgagcc
     7321 agaccggcgg ccacggcgac gactggtatc cgtcgggcgc tgaggtgccg ttcatgggcc
     7381 cgcacccggg ccgaccgtcc gggatcgtcg acggcgtgga cgaaatgcgc cgcaaggtcc
     7441 gtgagctcca tcgcgctggc gctgacgtga tcaaggtggc cacgtccggt ggcgtgctct
     7501 cggcccgcga cgacccgcgc cacgcccact tccggccggc cgagctcgac gccctcgtcg
     7561 aggaggcaac tgcggccgga atgttcgtca tggcccacgc ccagggcgcg gacgggatca
     7621 agaacgcggt ccgggccggg atccgctcga tcgaccacgg gatctacctc gacgacgagg
     7681 cgatcgagct gatgctccgc cgcgggacct ggttcgtgcc gacgctcgtc gccccacagg
     7741 gcgtcctcga cgcggtcgac gccggggtga gcctgccggc ggcggtggtc gacaaggccc
     7801 ggatggtcat cgacatccat cgagcggcct tccgccgggc ggtcgaagcc ggcgtccgga
     7861 tcgcgatggg caccgacagc ggggtcacgc cgcacgggcg caacctgcgg gagctccagc
     7921 tgatggccga cggcgggatg ccgcccgcgg cggtcctcga ggcgacgacg cgctcggccg
     7981 ccgagctgat gggcgtcgac gacgacctcg gcacgatcgc cgagggcaag ctcgccgacc
     8041 tcgtggtcgt cgcccgcgac ccctacgact tcaccgacct cggcgaccgg gtcgagcagg
     8101 tctggcaggg cgggaagcgc gtcgtctaga ggatggggcg tccgcgaagc gcggtttccc
     8161 gatgcaccag gcgactcaga tcggggatca gcacgctcgg ccgtgcacga accaggccac
     8221 ggccacgctc gctgcgaccg aatccgatgc accacgtgag tcaggtgcgc cgacaggaga
     8281 gctgtcacag ccgcagctga tcatcgggtc atgttccgac gcgccgaccc gctccgtatc
     8341 caggccgctc gccgcgcggc cgcgatcgcc cgcttgatca gcgccggcga gcttcccgat
     8401 cgggcggcgg tggccgtggc ccgatgggag gcgacgatcg acgggccggc ggatcgcacc
     8461 gactgggagg cgttcgaggg ctggctcgcc ggccgccggc cacggacctg aggcgagccg
     8521 gccccggtcg atccggtcgg tggctagacc tcggcgccgg ccgcctcggg ctgcggttcc
     8581 tgtgctcgct cgggtgcggc ttgcgtggcc cgggtgaccg cgcgggtcag gagctcgaag
     8641 atcggggcga gttggccgac gagctggctg ttctcgccat agatcgaaag gtttgtgccc
     8701 tggagcaccc tggatgcggc cccgaccatc tgtggcgcct gctcaagagg cagcatctcg
     8761 cgccgcagat cctgctgtgc ccggggcagc aggaggtcgc gttcgacgtc gagggtcttg
     8821 acctggttct ggaggtgtcg cagctcgagc tcctcggcga ggatcccgcg ctccttctcg
     8881 atccggagca ggcgcaccgg ggcttcggca ttcacgcggt ccgcgtcgac tgcgagctga
     8941 gcgtggagcc gctcctgctc gagttcgagg gcctggcgct gggtcgcgag gttctccgtc
     9001 tgctcgcggc gctggcgggc gatccgcgcg agctccaact gctcctctcg gcgcaggctg
     9061 tcctggtcgc cggccagctt ctgctccctg atgcgactct cggatgcc
//
LOCUS       DATAIW010000119         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_181402, whole genome shotgun sequence.
ACCESSION   DATAIW010000119 DATAIW010000000
VERSION     DATAIW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181402"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..>1856
                     /locus_tag="VHM48_03995"
     CDS             200..>1856
                     /locus_tag="VHM48_03995"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX2754596.1"
                     /translation="MAFVDVATGWAAGSGVILGTTDGGKSWRPEWTGTRSILSLSAVD
                     RLHAWGLGYGDLSVAADRLVRTTDGGRSWTMTKLSGGFREIAFGTDRVGWAVVGGITD
                     TTAAHGRLWETVDGGLHWRSSALTAGVDSVCFASPGLGWAAGGSNVYRTVDGGGRWTI
                     VAHGPSDAIEAAWSFATIRCRGNGAWVLSTGGAAAGSEGYLVTRTLDRGAHWRTVLGQ
                     LGDASATLPAIDAYAGSFDVASASAAGFIGFCPACGYGDWSYTRTTDGGRTFTHTPLA
                     GLTGASLRDLTFADPAHGWIAGSAAGGFLLATTDGGRTWHRAYPFSAPWPALDIALVS
                     PSVGFGLGVIGDGRAILRTDDGGRTWQAIGRLPVDPGARDRDPILSFVDPEHGWVATA
                     TGVLATTDGGHTWRSVPGAPPGGVAFADAEHGCAGSFDTPAAMTTDGGTTWAPVDAGR
                     GVVACAASLLDPAWARAAQPFDPGNLLTIGAVVDATHAWALGSLDADRIGLESTTDGG
                     TTWTAYRWPSPAGGTGGTVSDTLVRVSFATPTAGWVFTLSGRLF"
BASE COUNT          236 a          680 c          669 g          271 t
ORIGIN      
        1 gccccagccc accgcgtcgg cctccgcccc tcagccgacg gacaccccgt ctactgcccc
       61 cgccactccg accccgacag ggacggcgac accgacggcg accgcgtcac cgggggaatc
      121 gccgcgccag acgcccacga tcatccgcac cgtcgacgcc acagtccatt cggactcgat
      181 cccgccggcg ctgtcatccg tggccttcgt cgacgtcgca actggctggg cggccggcag
      241 cggcgtgatc ctcggcacga ccgacggcgg gaagagctgg cggcccgagt ggaccggcac
      301 gcgctcgatc ttgtcgctct cggcggtcga tcggctccac gcatgggggc tcgggtatgg
      361 ggacctgagc gtcgccgcgg accggctcgt gcggacgacc gacggcggtc gcagctggac
      421 gatgacgaag ctgagcggcg ggttccgcga gatcgccttt gggaccgacc gggtcggctg
      481 ggccgtcgtc ggcgggatca ctgacacaac agccgcgcac ggcaggctgt gggagaccgt
      541 cgatggcggc ctgcactggc ggtcgtcggc tttgacggcc ggggtcgact ccgtctgctt
      601 cgcgagcccg ggcctcggct gggcggcggg tggctccaac gtctatcgga cggtcgatgg
      661 cggcgggcgg tggacgatcg tcgcccacgg accaagcgat gcgatcgagg ccgcgtggtc
      721 cttcgccacc atccgatgtc gggggaacgg cgcgtgggtc ctctcgaccg gcggtgcggc
      781 cgctggcagc gaagggtatc tcgtgacgag aacgctcgat cgcggcgcgc actggcggac
      841 cgtcctcggt cagttgggag acgcgtccgc caccctgcca gccatcgacg catatgccgg
      901 atcgttcgac gtcgcctcgg cgagcgccgc cggcttcatc gggttctgcc ccgcgtgcgg
      961 gtacggcgac tggtcgtata cccgcacgac cgacggcggt cggaccttca cccatacgcc
     1021 cttggccggg ctgacgggag cgtcgctgcg cgacctcacc ttcgcggatc ccgcgcacgg
     1081 ctggatcgcg ggcagtgccg cgggcgggtt cctcctcgcg accaccgacg gcgggagaac
     1141 gtggcatcga gcctacccgt tcagcgcacc atggccggcc ctcgacatcg ccctcgtgtc
     1201 tccgagcgtc ggcttcgggc tcggtgtcat cggcgacggg cgcgcgatcc tgcggacgga
     1261 cgacggcgga cgcacctggc aggcgatcgg tcgtctgccg gtcgatcccg gggctcgcga
     1321 ccgcgacccg atcctctcgt tcgtcgaccc agagcacggc tgggtcgcga ccgccactgg
     1381 cgtgctcgcg acgaccgacg gtggccacac gtggcgcagc gttcccggcg cgccgcccgg
     1441 cggggtcgcc ttcgcggatg ccgagcatgg ctgcgccgga tcgttcgaca ccccagccgc
     1501 gatgacgacg gacgggggca cgacgtgggc gccggtcgac gcgggccggg gtgtcgtcgc
     1561 ctgcgcggca agcctcctcg atccggcctg ggcccgggcc gcgcagccgt tcgacccggg
     1621 caacctcctg accatcgggg cggtcgtcga tgcaacccac gcctgggccc tcggttcgct
     1681 cgacgctgac cggatcggcc tcgagtccac gacggacggc ggcacgacct ggaccgccta
     1741 ccggtggccg tcacccgcgg gtggaaccgg cggaacggtg tctgacactc tcgtccgcgt
     1801 gtcgttcgcc actccgaccg ccggctgggt gttcacgttg tccgggcgcc tgttcg
//
LOCUS       DATAIW010000120         5239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_5459, whole genome shotgun sequence.
ACCESSION   DATAIW010000120 DATAIW010000000
VERSION     DATAIW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5459"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="VHM48_04000"
     CDS             complement(<1..2603)
                     /locus_tag="VHM48_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2754597.1"
                     /translation="MQTSLARRQRHRRSLDRGRPRGSPTIRRIAIAIPMVLFLAFLAV
                     GLAGVIGIAVAYNYYSQGLPDPRDTLSNLSFDQQTVLTDRTGKVRLASLGEFKREVVT
                     FDQIPPEMLDATTAIEDKDFWSNPGFDAAGFVSATLDTLSGHPRGGSTITQQLVRARL
                     LPESAFIGSREERKIREIIQSLRLTEAYPGNVGKQEIITAYLNQNFYGNQSYGIKAAA
                     HTYFGKELRDLTLAQEALLAAIPQSPSTYDLVKNADDVCTVKIKDGADCPAGKNELEV
                     PARTAIVIRRNFVLDLMKTRSTLSGSKHTVDEYEAAKNELVILAPQIGVNWKAPHFVW
                     QVRSDLAAILCPDVPADQCNDIDTGGFRVTTTLDWKMQATVEKWLYAAARAPNLPNTL
                     AKLKSLKIPGKDWGWLQDLKSANIHNGAAAVLDYRTGQVLAYAGSASYIAKGTKKFQP
                     KWDVMSDGWRQPGSSIKPINYSIGIEDQTMTAATMFMDVTTDFGGKFVPTQADQKERG
                     PVRLRSALQFSLNIPSIKAGIMNGLDHFYHRAQDFGLHWIPGSGPVVSMSIGTIEIHP
                     IDLTSAYGTIANGGKRLPRTTILKVTRADGTVVWPDASTKKPKAKTVISPQTAYIITD
                     ILAGNTELKTNPFWGKWAVYDHGVRRPAAYKTGTTNENRDVLAFGFLAPPKDPKAPAL
                     VVGVWMGNSNNAPDKDTLSLGSSAPLWSRILTEVSHGTPIVDFKQPKGLVTATVDAFS
                     GMKPGPFTNKRVKELFIAGTEPTQSDDLHRSVVIDAASGLRWRDGCVGPMKTVGALDF
                     SQVEAGHANWQKADNGWARRAARGSGVGGGLKGSATQYFYGGGPPFYPFGRSWGGIFA
                     PTRLCPLAPPPT"
     gene            2723..4021
                     /gene="tyrS"
                     /locus_tag="VHM48_04005"
     CDS             2723..4021
                     /gene="tyrS"
                     /locus_tag="VHM48_04005"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2754598.1"
                     /translation="MAGLGGLLDELTWRGMLHATTPGLPGRLASGRTIAGYNGFDPSG
                     PWLHIGHLIPIMGLLQLQRHGGRPVALVGGGTGMIGDPSGASAERNLLDRETLAANVA
                     SIRTQLERFLDFAGATGAVMVDNLDWLAGIGLIDWLRDVGKHFTIPYMLAKDSVQVRL
                     DRGLSFTEFSYMLIQATDFEHLHRTMGVELQMGGADQWGNITAGLELIRRRAGGGDSE
                     ADRDADEPAHGLAYKLLLSPSGTKFGKTAGGETIWLAPEQTSPYTFYQYWLNVDDRDV
                     GTYLRWFTLLARAEIDELERDLVARPEARAAQRALASDVTERTHGSSARDHAARVSAA
                     AFSGEPIRDPGVLETLHGSIGGFAFSDADVAGGALAIAVASGLYASAGEARRAIAQGG
                     FTIDDERIVAIDAPVPPPVDGEWLVLRAGKRRIAVGRRRT"
     gene            4257..5003
                     /locus_tag="VHM48_04010"
     CDS             4257..5003
                     /locus_tag="VHM48_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2754599.1"
                     /translation="MARRRRPAAEIAGAREAQAIAATLGREARSTRRRRRLTQAELGR
                     RVGLSQSEISHLEAGHGQGTSIETWTAIGIALGRPLTIGFGRDIVPTPRDAGHLAGQE
                     LLLRLAPAHGRVGQFELPTRPFNPSLSIDVCLRDLRNRVLIVAEIWNRLDDLGAAARN
                     TSRKVAEAKILAATHDPPDRVAWCWLFVDTAANREIVRTWPAVIRAQFDGSSQGWVRA
                     LASGATPPDRPGIAWIDLREGRLRELRLAG"
     gene            complement(5009..>5239)
                     /gene="hisE"
                     /locus_tag="VHM48_04015"
     CDS             complement(5009..>5239)
                     /gene="hisE"
                     /locus_tag="VHM48_04015"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX2754600.1"
                     /translation="GGVDAAGRKVTEEASEVLIAAKDDATAETSGANRTATRAALAGE
                     TADLLFHALVLIAERDVSPSAVIDVLRGRHSG"
BASE COUNT          760 a         1711 c         1878 g          890 t
ORIGIN      
        1 gtcggtggcg gcgcgagcgg acagagccgg gtgggggcga agatgccgcc ccacgatcgg
       61 ccgaacggat agaagggcgg gccgccgccg tagaaatact gggttgccga gcccttcaat
      121 ccgccgccga cgccggatcc gcgggctgcc cgcctggccc agccgttgtc ggccttctgc
      181 cagttcgcgt ggcccgcttc gacctggctg aagtcgagcg cgccgacggt cttcatcggt
      241 ccgacgcagc cgtcccgcca gcggagaccg gacgccgcgt cgatgacgac cgaccgatgg
      301 aggtcgtccg actgggtcgg ctccgtgccg gcgatgaaca gctccttgac cctcttgttg
      361 gtgaacggac ccggcttcat cccgctgaac gcatcgaccg tcgcggtgac gaggcccttc
      421 ggctgcttga agtcgacgat cggcgtgcca tggctgacct cggtcaggat ccgcgaccag
      481 agcggcgccg acgacccgag cgagagggtg tccttgtcgg gggcgttgtt gctgttgccc
      541 atccatacgc cgaccacgag agcaggcgcc ttggggtcct tcggcggggc gaggaagccg
      601 aaggcaagga cgtcgcggtt ttcgttcgtg gtcccggtct tgtaggccgc ggggcggcgg
      661 acgccgtggt cgtagacggc ccacttgccc cagaatgggt tcgtcttcag ctcggtgttg
      721 ccggcgagga tgtcggtgat gatataggcc gtctgcgggc tgatcacggt cttcgccttc
      781 ggcttcttcg tcgaggcgtc cggccagacg accgtcccgt cggccctggt gaccttgagg
      841 atcgtcgtcc gcggcagccg cttcccgccg ttggcaatcg tgccataggc gcttgtcagg
      901 tcgatcgggt ggatctcgat cgtcccgatc gacatcgaga ccaccggccc ggagccgggg
      961 atccagtgga ggccgaagtc ctgggcccgg tgatagaagt ggtcgaggcc gttcatgatc
     1021 ccggccttga tcgacgggat gttcagggag aattggaggg ccgagcggag acgaaccgga
     1081 ccgcgctcct tctgatcggc ctgggtcggg acgaacttgc cgccgaagtc ggtcgtgacg
     1141 tccatgaaca tcgtggcggc cgtcatcgtc tgatcctcga tcccgatcga gtagttgatc
     1201 ggcttgatcg acgagccggg ctgtcgccag ccgtcggaca tcacgtccca cttcggctgg
     1261 aacttcttcg ttcccttcgc gatgtagctc gccgacccgg catacgcgag gacctggccc
     1321 gtccggtagt cgaggaccgc ggccgcacca ttgtggatgt tcgcgctctt gaggtcctgg
     1381 agccagcccc agtccttgcc cgggatcttg agggacttga gcttggcgag ggtgttcggc
     1441 aggttggggg cccgggccgc ggcatacagc cacttctcga ccgtcgcctg catcttccag
     1501 tcgagcgtcg tcgtcacccg gaagccgccg gtgtcgatgt cattgcattg atcggccggg
     1561 acatccgggc agaggatcgc cgcgagatcg ctgcggacct gccagacgaa gtggggagcc
     1621 ttccagttga cgccgatctg gggggcgagg atgacgagct cgttcttggc cgcttcgtac
     1681 tcgtcgacgg tgtgcttcga gccggagagc gtgctgcggg tcttcatcag gtcgaggacg
     1741 aagttgcggc gaatcacgat agccgtcctg gccgggactt cgagctcgtt cttgccggcc
     1801 gggcagtcgg cgccgtcctt gatcttgacg gtgcagacat cgtcggcgtt cttgaccagg
     1861 tcatacgtcg agggtgactg cggaatggca gccagcagcg cctcctgggc gagggtcagg
     1921 tccctgagct ccttgccgaa gtaggtgtga gctgccgcct tgatcccgta cgactggttg
     1981 ccgtagaagt tctggttgag gtaggcggtg atgatctcct gcttgccgac gttgcccgga
     2041 tacgcctcgg tcaggcgaag ggactggatg atctcgcgga tcttccgctc ctcgcggctg
     2101 ccgatgaagg cgctctccgg caggagccgt gcccggacga gctgctgggt gatcgtcgag
     2161 ccgccacggg gatgaccgct gagcgtgtcg agggtggccg acacgaaacc ggccgcgtcg
     2221 aaccctggat tcgaccagaa gtccttgtcc tcgatcgcgg tcgtggcatc gagcatctcc
     2281 ggcgggatct ggtcgaacgt gacgacttcg cgcttgaact cgccgaggct cgccagcctg
     2341 accttgccgg tccggtcggt caggacggtc tgctggtcga aggagaggtt gctcagggtg
     2401 tcgcgcggat ccggcaaccc ctggctgtag tagttgtacg cgacggcgat cccgatgacg
     2461 cccgccaggc cgaccgcgag gaaggccagg aacaggacca tggggatcgc gatcgcgatc
     2521 ctccggatgg tgggggagcc gcggggccgc ccgcggtcga ggctgcgccg atggcgctgc
     2581 cgccgcgcga ggctggtctg catgtcgggc cgcacgatag cataggagcc gtgaggagcc
     2641 ccttcttcct acccgtcgtg acggctcggc cgacgagcct gtgacgctcc cggatggtgc
     2701 tgacgggccc gctcggagcg aggtggccgg cctcggcggc ctgctcgacg agctgacctg
     2761 gcgggggatg ctccacgcga cgacgccggg tctcccgggg cgtctcgcca gcggccggac
     2821 gatcgccggt tacaacggct tcgatccctc cgggccgtgg ctccacatcg gccacctgat
     2881 cccgatcatg ggcctcctcc agctccagcg ccacggcgga cgcccggtgg cgctggtcgg
     2941 gggcggaacc gggatgatcg gtgatccgtc gggcgcgtcc gccgagcgga acctgctcga
     3001 tcgcgaaacg ctggctgcca acgtcgcctc gatccgcacc cagctcgagc gcttcctcga
     3061 cttcgccggt gccaccgggg cggtcatggt cgacaacctc gattggcttg ccgggatcgg
     3121 gctgatcgac tggctgcgcg acgtgggaaa gcacttcacg atcccgtaca tgctggccaa
     3181 ggactcggtc caggtccggc tcgaccgcgg gttgtcgttc accgagttca gctacatgct
     3241 catccaggcg accgatttcg agcatctcca ccggacgatg ggggtcgagc tccagatggg
     3301 cggcgctgac cagtggggca acatcaccgc cggtctggag ctgatccgac ggcgcgccgg
     3361 gggaggggac agcgaggccg atcgcgacgc ggacgagcct gcccatgggc ttgcgtacaa
     3421 gcttctgctg tcgccgtcgg gcacgaagtt cggcaagacc gccggcggcg agacgatctg
     3481 gctcgcgccg gaacagacct cgccgtacac gttctaccag tactggctca acgtcgacga
     3541 tcgcgacgtc gggacgtacc tgcgctggtt cacgctgctg gcgcgggccg agatcgacga
     3601 gctggagcgc gacctcgtcg cccggcccga ggcgcgcgcg gcgcagcggg cgctcgcgag
     3661 cgacgtcacc gagcggacgc acggctcgtc cgcccgcgac catgccgctc gggtgagcgc
     3721 ggcagcgttc tcgggcgagc cgatccggga tcccggcgtg ctcgagacgc ttcatggctc
     3781 gatcggcggc ttcgcgttct ccgatgcgga cgtcgcaggc ggcgcgctgg cgatcgcggt
     3841 cgcatccggg ctctacgcct ccgccggcga ggcgcgccgg gcgatcgcgc agggcgggtt
     3901 cacgatcgac gatgagcgga tcgtcgcgat cgacgcaccc gtcccgccgc ccgtcgacgg
     3961 ggagtggctc gtgctgcggg ccggcaagag gcggattgcc gtggggcgtc gccgcacgta
     4021 gcggcgaagg gcgctcgcgc ccagagcctg gcgcggctcg ccagggcccg cctgcatcga
     4081 ccccatcgat attccctggc ttgccggacg aacaggcgga ttccgaaggc cggaacggga
     4141 caaccatcgc gttggccacg cgtgggcgac ggtcacagca acttgtctct gcaatattcc
     4201 cgcggcaagc ggccgccaat cggtggtgca gagccatcgg taacgatggc gtcgatatgg
     4261 cacgacgtag acgcccggcg gcggagatcg cgggagctcg cgaggcccag gccatcgcgg
     4321 ccactctcgg acgcgaggcc cggtcgaccc gacgacgacg tcgactcacg caggcggagc
     4381 tcgggcgtcg agtggggctg agccaatcgg agatcagtca cctcgaggcg ggccacgggc
     4441 agggcacgtc catcgagacg tggacggcga tcgggatcgc tctcgggcgg ccgttgacga
     4501 tcggcttcgg ccgggacatc gtgccgacgc cgcgcgatgc cggacacctc gccgggcagg
     4561 agctccttct gcggctcgct ccggcgcacg gtcgagtcgg gcagttcgag ctgccgacgc
     4621 ggccgttcaa cccatcgctg tcgatcgacg tctgtctgcg cgaccttcgg aaccgcgtcc
     4681 tgatcgttgc cgagatctgg aaccggctcg acgacctcgg ggctgcggct cgcaacacgt
     4741 cgcgaaaggt cgccgaggcg aagatcctcg cggccaccca cgatccgccc gatcgcgtcg
     4801 cctggtgctg gctcttcgtg gacacggccg ccaatcggga gatcgtccgg acctggccag
     4861 cggtgatccg cgctcagttc gacggatcgt cacagggatg ggttcgtgcc ctggcgagtg
     4921 gcgccacgcc gcccgatcgg cccggtatcg cctggatcga tctgcgtgag ggtcggttga
     4981 gagagcttcg gctcgcgggt tgatccggtc agccgctgtg gcggccgcgc aggacgtcga
     5041 tcaccgccga cgggctcacg tcgcgttcgg cgatcaggac cagggcgtgg aagagcagat
     5101 cggcggtttc gccggccagg gcagcgcggg ttgcggtccg gttcgcgccg gaggtctcgg
     5161 ccgtcgcgtc gtccttggcg gcgatcagga cctcgctcgc ctcctcggtc accttgcgac
     5221 cggcggcgtc cacgccgcc
//
LOCUS       DATAIW010000121         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_95269, whole genome shotgun sequence.
ACCESSION   DATAIW010000121 DATAIW010000000
VERSION     DATAIW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95269"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..388)
                     /locus_tag="VHM48_04020"
     CDS             complement(149..388)
                     /locus_tag="VHM48_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754601.1"
                     /translation="MSGYIAAGRVVRASPRLAAAARTVERATDGGTPAATEARIVEGD
                     GGGGHAPSRLSERRSALRERWSQLTFYLFDPNSWR"
     gene            454..1320
                     /locus_tag="VHM48_04025"
     CDS             454..1320
                     /locus_tag="VHM48_04025"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX2754602.1"
                     /translation="MTTEGRYGAHSSTAPLRDVLVKRPGPAFGRAFDDPAHGFLRPVD
                     LERARREHDGLVAALDGLGVHVHVLDVEPSTDPDLVYAFDPLLVIDAGAVPLRPGKLN
                     RADEPAIMERWLNAHDIPTVGRIGAPGTVEAGDTVWLRDELFVIGRTLRTNDAGARQL
                     AAIVGGDTRIVDVPYWKGPAELVHLMSVISPISDELAVVFLPLLPAGLYGWLMELGIR
                     LIEVPGEEYPTLGCNVLAVRPGVVVVADGNAVTRRGLESAGVEVHAVALGEVGENGSG
                     GVTCLTRPLLRG"
     gene            1290..2594
                     /locus_tag="VHM48_04030"
     CDS             1290..2594
                     /locus_tag="VHM48_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX2754603.1"
                     /translation="MPDSTAAPRLMATGLSDAARAAADAVDPAAIRDELAAIVRIPSV
                     TGREEAVAGELAGRAREAGLAVELLTPDPAAIRADSAWPGEETERTSLPVVIGRIGRP
                     GGRRLVLSGHIDVVPPGDPATWMVDPWGGEVRDGRLHGRGACDMKGGVAAILGAVRAL
                     VASGAAARLAGELVVALVPGEEDGGQGTLAAIRAGVRGDLAIITEPSNLEIVVAHAGA
                     ITFRLTVPGRAAHASQRREGVSALDNLYVVLRALEADETARNASETDPLMTVLGLPYP
                     TIVGIVAGGEWASTVLDRVVVHGRYGVRLGQSPANAAADLRRAIDAANESDPFLSEHP
                     ATVEIVGGTFGSGRIPSEHPLPVGLADAAEAVTGRRPALLGEPYGADMRLFINEGDTP
                     CVMYGPGDVKVAHGADEHVSLAEVEECARVLAAWVVRELGVG"
BASE COUNT          367 a         1061 c         1072 g          400 t
ORIGIN      
        1 atccacagcc cgtcggccca tggggcatct cgaccggtcc gggctcggct cgcctgctcg
       61 ccgagcggtt cctcggccgt tcggcggagg atgcgatccc gccggcgctc gaggtcggcc
      121 gcttcggcgt gcccggtgcc gtccccggct agcgccagga gttcgggtcg aacaggtaga
      181 aggtgagctg cgaccagcgc tcgcgtaatg cgctgcggcg ctcggaaagg cgactcggtg
      241 cgtggccgcc gccgccgtca ccctcgacga tccgcgcctc cgttgcggcg ggggtcccac
      301 cgtcggtcgc cctctcgacg gtccgcgcgg cagcagccag ccgcggcgat gcccggacga
      361 cccgcccagc cgcgatgtac ccgctcatcg cggcagacta atcctcggga cggcgatcgg
      421 gaaggggagc ggtcgtcgta tcctccgccg gtgatgacga ccgagggtcg ctatggagcg
      481 cacagctcga ccgcaccgct ccgcgacgtt ctcgtcaagc ggccgggtcc ggcgttcggt
      541 cgggcgttcg acgatcccgc ccacggcttc ctccgaccgg tcgacctcga gcgagcccga
      601 cgcgagcacg acggcctggt cgccgcgctc gacgggctcg gggtccacgt ccatgtcctc
      661 gatgtcgaac cgtcgaccga tcccgacctc gtgtacgcct tcgacccgct cctcgtcatc
      721 gacgcggggg ccgtcccgct ccggcccggc aagctcaacc gggccgacga gccggcgatc
      781 atggagcgct ggctcaacgc ccacgacatc ccgacggtcg ggcgcatcgg ggcacccggg
      841 acggtcgagg cgggcgacac cgtctggctc cgcgacgagc tgttcgtcat cggccggacc
      901 ctccggacca acgacgccgg ggcccgccag ctcgccgcga tcgtcggcgg cgacacccgg
      961 atcgtcgacg tcccgtactg gaagggtccg gccgagctcg tccacctgat gtcggtgatc
     1021 tcgccgatca gcgacgagct cgccgtcgta ttcctgccgc tgctgccggc ggggctgtac
     1081 ggctggttga tggagctcgg gatccggctg atcgaggtgc ccggggagga atacccgacc
     1141 ctcggctgca acgtcctcgc ggtccggccc ggagtggtcg tcgtcgccga cggcaacgcg
     1201 gtgacgcgac gcggtctcga gtcggccggc gtcgaggtcc acgccgtcgc gctcggcgaa
     1261 gtcggcgaga acggctcggg cggggtgacg tgcctgactc gaccgctgct ccgcggctga
     1321 tggcaacggg cctgtcggac gccgcccggg cggccgccga cgccgtcgat ccggccgcca
     1381 tccgggacga gctcgccgcg atcgtccgga tcccgagcgt caccggtcgc gaggaggccg
     1441 tcgccggcga gctcgctggt cgcgcccggg aagccggcct cgccgtcgag ctcctgacgc
     1501 ccgacccggc ggcgatccgg gccgattccg cgtggcccgg ggaagagacc gagcggacct
     1561 cgctgccggt cgtcatcggc cggatcgggc ggcccggcgg ccggcggctg gtcctgtccg
     1621 gccacatcga cgtcgtcccg cccggggatc cggccacctg gatggtcgat ccgtggggtg
     1681 gcgaggtccg cgacggccgc ctgcacggcc gcggcgcctg tgacatgaag ggtggcgtcg
     1741 cggcgatcct cggtgcggtc cgggcgctcg tcgcgagcgg cgccgccgcg cggttggccg
     1801 gcgagctcgt cgtggccctc gtgccgggtg aagaggacgg agggcagggg accctggccg
     1861 cgatccgggc cggcgtccgc ggcgacctcg cgatcatcac cgagccatcc aacctcgaga
     1921 tcgtcgtggc ccatgccggg gcgatcacgt tccggctgac cgtgccgggc cgggccgccc
     1981 acgcgagcca gcgccgggag ggcgtgtcgg cgctcgacaa cctctacgtc gtcctgcgcg
     2041 cgctcgaggc cgacgagacc gcccgcaatg catccgagac cgacccgttg atgacggtcc
     2101 tcggcctgcc ctatccgacg atcgtcggga tcgtggcggg tggcgagtgg gcgtcgaccg
     2161 tgctcgaccg agtcgtcgtc cacgggcggt acggcgtccg gctcggccag agcccggcga
     2221 acgcggcggc ggacctgcgg cgggcgatcg acgcggccaa tgagtcagac cccttcctaa
     2281 gcgagcaccc ggcgacggtc gagatcgtcg gcgggacgtt cggatcgggt cggataccgt
     2341 ccgagcaccc gttgccggtc ggcctcgcgg acgccgcaga ggccgtcacc ggccgccggc
     2401 cggccctcct cggcgagccg tacggggcag atatgcggct gttcatcaac gagggcgaca
     2461 cgccctgcgt catgtatggc ccgggtgacg tcaaggtcgc ccacggcgcc gacgagcacg
     2521 tatcgctcgc cgaggtcgag gaatgtgccc gggtcctcgc cgcgtgggtc gtccgcgagc
     2581 tcggggtcgg gtagggcgcg acgggccgag ctggggatat cggtgtcccg ctgtgcgcgg
     2641 ggcgagccta tccgacgcga atgtcatccc ggcgggcccg aacgaccggg aatcgcacga
     2701 gatccacgcc cgacgatcgc cgtcgaccga ttccgggccg aacgcgtcca aggctgtgcc
     2761 cagcgcgtct cacgtgctca ccgggtgaag acggctcgcg ccgagcccgg gccgagctcc
     2821 ggccggccct cgcgcgaccc gatttcgggt caggccgctg cgtgccccga aatcgggtcg
     2881 cgcgagggcc ggccggagct
//
LOCUS       DATAIW010000122         7664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211110, whole genome shotgun sequence.
ACCESSION   DATAIW010000122 DATAIW010000000
VERSION     DATAIW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211110"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="VHM48_04035"
     CDS             complement(<1..1436)
                     /locus_tag="VHM48_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754604.1"
                     /translation="MIRMPRARPIALRWLVAIACLAAGAVAIGGGRLGGESAARSVVA
                     TADLALASPPAQAPVASTATPPASPSTTQPAVARAPSPSVGPGGVVAPTTDGVVPGTV
                     NATSINLSAEYVATVRLSFATRSFHVSSTMTVTNTSGKAIDRLELNTIAARLGHMTLT
                     LARVDGVAVHATVSDQTIHLALGGILEPGATTTVRIGYLATLRSDTAGSNWLFTRANG
                     ILEAHRWLPWISIPTAFDRPNHGDPFVTPVSPRVRVTIVSDRAIRWATTGEQTSRTGS
                     TTSFEARNVRDFVFAGAPDYKTVSATVGAVLVHVWYRPGFPALAVLSAAKTALAREAK
                     LLGAYPYRTYDLAQTAGGYGMESPGLTWIPTGAGNLPYLVAHETGHQWFYGIAGNDQA
                     RQPYADEAATDFVARYVLGLRRGSRCATARLDLTIYQYSSACYYEDIYIQGGNFLDDT
                     RKVMGSTAFWAGMRDYLARYRFKIGGTKA"
     gene            1493..2923
                     /locus_tag="VHM48_04040"
     CDS             1493..2923
                     /locus_tag="VHM48_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2754605.1"
                     /translation="MATTDRPRSIAATDPIDPVAGARLLDAIRSTLPDVRLLADPVDR
                     ESYRNDETAYLSAGLPLAVVLPETTSEVATIVRLAAAQRVPVVPRGAGSGLSGGAAGI
                     EGGLTIALTRMSRVLEIDRANLVVVTQPGVLNADLKAAVAAEGLFYAPDPASYETCSI
                     GGNLGTNAGGLCCVKYGQTRDSVLGLEVVLADGRVIRTGGRSVKDVAGYALTHLFVGS
                     QGTLGIITEATLRLHIAPPPRSTMLAFFASLPAAGRAVAAIIGAGIHPVTLELLDHET
                     ILAVDDAFKLGLERNAAAMLLVESDLPAAAASAELETAVAACEVAGATSVVRAEDPTE
                     ADWLRQARRLALRALEQQGIVRMEDVGVPRGRVPELLVAIQAAADRHGVRVATFGHAG
                     DGNLHPNFIFARDDPRAAELTELVRDEIFAAALALGGTVTAEHGIGLSRRAALVDQVG
                     PDVIDVMRSIKAALDPLGILNPGRVI"
     gene            2962..3876
                     /locus_tag="VHM48_04045"
     CDS             2962..3876
                     /locus_tag="VHM48_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754606.1"
                     /translation="MVVAARISGVILAVAACTTSPVVTSPGPSHGLDASAGPSVDAGV
                     AIPRILILSTDGTLSIYDPQQPAPQLPRRLIVPPGRWNLTSGPASVALTSLDPAGSRT
                     ILLGTIPDAAFVGDEQITLPAGDPWQGGYAACLSGAGPILAADAGLALWLIAPGEAPV
                     KLPNQGNNTGGCAWLDDSRVVWEQENDRLATFDLEAAKTSELPATDIVGDPSGGRGRI
                     AGRTAQDGLDVRAYTLDGSSVKIGPSVGRIDPEVGGSLSADGRWLVTAGPDPLARVYR
                     VDETSLTAFGSFGIGADDRVEWMPLPGP"
     gene            3925..5313
                     /locus_tag="VHM48_04050"
     CDS             3925..5313
                     /locus_tag="VHM48_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2754607.1"
                     /translation="MTHARRLARPCLPPIAIALLSVAIVACGEPRAPSSPAGSGSAAR
                     ASPPPSAPSAPSGGPEASAISSSPSSPSVASTAAEIARLEAVVAADPTDAGSQRDLGF
                     ALAQRVRETADPSLYAPAEAAFRAALRLRPDDAPALVGIAGIQLGKHEFAAGLETARR
                     ALALSPSLVAAHAAQVDALVELGRYDEADTAAGAMFGLSSDLSTLARVSYLAELRGRL
                     PAALTAMRAAARAPGLAPENTAYIDALLGNLLVANGDPDGAATAYASALVLVPEHAPS
                     LAGGGRLAVGAGHLDEAIERFEHAARVVPLPEYVIALGDAQTAAGQADGAVRSYKLAR
                     AEIQLFKAAGVVVDVDLALLEADHGDPAAALSYAMAALAVTPTVRAADAVGWALHRLG
                     RNREALAQVKDALRLGSIDPILRYHAGAIEASLGKAVDARRDLTRALATDSGFSATGA
                     AEARRLLASLKG"
     gene            5685..7193
                     /locus_tag="VHM48_04055"
     CDS             5685..7193
                     /locus_tag="VHM48_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX2754608.1"
                     /translation="MSTRSKAAALTGALILATSSVGGILASSHREAPLIAGDPAADNT
                     DLYAFLDPNDNTKLTIIANYVPLEEPAGGPNFFPFDPNVRYEIKIDNTGDGVADVTYR
                     FKFKTRSQATNFAGIPTFLYNDGPVTSLADPNLLVKQTYSIERNGVAIGSGLRTPPVN
                     VGPRSTPDYAMTAGQAIYTLGDGMKVFAGQRDDAFFVDLGSIFDLAGLRNFNALHAIP
                     LPAAAGVDGVGGFNTNSIAIDVPLNLLTKDHHQPTGPNDPDAVLGIWATASRQQTRKL
                     NAIGTMSFSGPWVQVSRLGNPLINEVIIPREKKDYWNGQTPNHDSQFAKYYKAPEVTA
                     VANVLYDALDTPATTNRNDLVAILLTGLDIPSSTVVPGGLHYTRTGTTQADLLRVNTG
                     IKPNAAGACVFGVAGGGSPSRLGALDGDLCGFPNGRRLADDVTDIELRALLEGYGPTL
                     NAVLGVPNRSPNNLIGDGVDENDVPFLAAFPFEGTPNQGYEHTHHRLGPIPI"
BASE COUNT         1085 a         2850 c         2634 g         1095 t
ORIGIN      
        1 gccttcgtgc cgccgatctt gaagcggtac cgggcgaggt agtcgcgcat cccggcccag
       61 aacgcggtcg agcccatcac cttgcgggtg tcgtcgagga agttgccgcc ctggatgtag
      121 atgtcctcgt agtagcaggc gctcgagtac tggtagatcg tcaggtccag gcgggccgtt
      181 gcgcagcgcg agccccggcg cagccccagg acgtagcggg ccacgaagtc ggtggccgcc
      241 tcatcggcgt agggctggcg ggcctggtcg ttgccggcga tcccgtagaa ccattgatgc
      301 cccgtctcgt gggcgacgag gtaaggcagg ttgccggccc cggtcgggat ccatgtcagg
      361 cccggcgatt ccatcccgta gccgccggcc gtctgggcga ggtcgtaggt ccggtacgga
      421 tacgcgccga ggagcttcgc ctcgcgcgca agcgcggtct tcgccgcgga aaggaccgcc
      481 aacgccggga accccggccg gtaccagacg tggacgagga cggcgccgac cgtcgccgac
      541 actgtcttgt agtcgggcgc gccggcgaag acgaagtcgc ggacgttgcg ggcctcgaac
      601 gacgtcgtcg acccggtccg gctcgtctgc tcgccggtcg tcgcccagcg gatcgcgcgg
      661 tcgctcacga tcgtgacccg gacgcgcggg ctcaccggtg tcacgaacgg atcgccgtgg
      721 ttgggccgat cgaaggcggt cgggatgctg atccacggca accagcgatg ggcctcgagg
      781 atgccattgg cccgcgtgaa gagccagttc gagcccgccg tgtcgctgcg cagcgtcgcg
      841 agatacccga tccggaccgt cgtcgtcgcg cccggctcga ggatcccgcc caacgccaga
      901 tggatcgtct ggtcggacac ggtcgcgtgg accgccacgc cgtcgacccg ggccaacgtc
      961 agggtcatgt ggccgaggcg ggcagcgatc gtgttcagct cgaggcgatc gatcgccttg
     1021 cccgacgtat tcgtgacggt catcgtcgac gagacgtgga agctgcgcgt cgcgaagctc
     1081 agtcggaccg tcgcgacgta ctcggccgac aggttgatgc tcgtcgcgtt gacggtgccc
     1141 gggacgacgc cgtccgtcgt cggagccacg acgccgccgg ggccgaccga cggcgacggc
     1201 gccctcgcca cggccggttg ggtcgtcgac gggctggcgg ggggcgtggc ggtcgaggcc
     1261 accggcgctt gcgccggggg cgatgccagc gcgaggtcgg cggtcgcgac gaccgacctt
     1321 gcggcggatt cgccaccgag cctgccgcca ccgatcgcga ccgcgccggc cgcgagacag
     1381 gcgatggcga cgagccagcg gagcgcgatc ggtcgggcgc gaggcatgcg gatcatcggg
     1441 gtcatgctag gccgggtggt ctcgatcggt cgaccgtcga tagactggcg cgatggcgac
     1501 gaccgaccga ccccgatcga tcgcggcgac cgacccgatc gatccggttg ccggggcccg
     1561 gctcctcgat gccatccgct cgaccctgcc ggacgtccgt ctcctcgccg atcccgtcga
     1621 tcgcgagtcg taccgcaacg acgagacggc gtatctcagc gccggcctgc cgctcgccgt
     1681 cgtgctgccg gagacgacgt ctgaggtcgc cacgatcgtc cggctggccg cggcgcagcg
     1741 cgtcccggtc gttccgcgtg gcgctggctc cgggctgagc ggcggcgcgg ccgggatcga
     1801 gggcgggctg acgatcgccc tgacccggat gagccgggtg ctcgagatcg accgggcgaa
     1861 tctcgtcgtc gtcacccagc ccggcgtcct gaacgccgac ctcaaggccg cggtcgccgc
     1921 ggagggcctg ttctacgcgc ccgacccggc gagctacgag acctgctcga tcggcgggaa
     1981 cctcggcacg aacgctggcg gcctgtgttg cgtgaagtac ggccagaccc gtgacagcgt
     2041 cctcgggctc gaggtcgtcc tcgccgacgg ccgcgtcatc cggaccggtg gccgctcggt
     2101 gaaggacgtc gcaggctacg ccctgaccca cctcttcgtc gggtcgcagg ggacgctcgg
     2161 gatcatcacc gaggcgacgc tccgcctcca catcgcgccc ccgccgcgct cgacgatgct
     2221 tgccttcttc gcgtccctgc ccgcggcggg tcgggcggtc gccgcgatca tcggggccgg
     2281 catccacccg gtcaccctcg agctgctcga tcacgagacg atcctcgcgg tcgacgatgc
     2341 gttcaagctg ggcctcgagc gcaatgcggc cgcgatgctt ctcgtcgaga gcgacctgcc
     2401 ggccgcggcc gcgtcggccg agctcgagac ggcggtggcg gcgtgcgaag tcgccggcgc
     2461 gacgagcgtc gtccgggccg aggacccgac cgaggctgac tggctgcgcc aggcccgccg
     2521 tctcgccctc cgggcgttgg agcagcaggg gatcgtccgg atggaggacg tcggcgtgcc
     2581 gcgcggccgc gtcccggagc tgctggtggc gatccaggcc gcggccgacc gccacggcgt
     2641 acgggtcgcg acgttcggcc atgccggcga cggcaacctc cacccgaact tcatcttcgc
     2701 ccgggacgac ccgcgcgccg ctgagctgac cgagctcgtc cgcgacgaga tcttcgccgc
     2761 cgccctcgcc ctcggcggaa ccgtgaccgc ggagcacggg atcggtctct cgcggcgggc
     2821 ggcgctcgtt gaccaggtcg gtccggacgt gatcgacgtc atgcgctcga tcaaggccgc
     2881 cctcgacccg ctcgggatcc tgaaccccgg ccgagtgatc tgatcggcac ggcgccgcgt
     2941 tccgctcgtg accgccgcac gatggtcgtt gcggcgcgga tctcgggcgt gatcctggcc
     3001 gtcgccgcct gcacgacgtc tccggtcgtc acgagccccg ggccgtcgca cggattggac
     3061 gcctcggctg gcccgtccgt tgacgccggg gtcgcgatcc cgaggatcct gatcctctcg
     3121 acggacggaa cgttatcgat ctacgacccg cagcagcccg ctccgcagct accccggcgc
     3181 ctgatcgttc cgcccggtcg ctggaacctg acgagcggcc cggcttcggt ggccctgacg
     3241 agccttgatc ccgccgggtc gcgaaccatc ctgctcggga cgatcccgga cgccgccttc
     3301 gtcggtgacg agcagatcac cctgcccgcc ggcgacccgt ggcagggcgg ctacgcggcg
     3361 tgcctgtcgg gggccgggcc gatcctcgcc gcggacgccg gcctcgcgtt gtggctgatc
     3421 gctcccggag aagctcccgt caagctgccg aaccagggca acaacaccgg cggctgcgcc
     3481 tggctcgacg acagccgcgt ggtctgggag caggagaacg atcgtctggc gacgttcgat
     3541 ctggaagccg ccaagacatc ggagctgccc gccaccgaca tcgtgggcga tccgtcgggc
     3601 gggcgcggtc ggatcgccgg ccgcacggcc caggacggac tcgacgtgcg cgcctacacg
     3661 ctggacgggt cgagcgtcaa gatcgggccg tcggtcggcc ggatcgatcc ggaggtcggt
     3721 gggagcctgt cggccgatgg gcgctggctc gtgaccgccg gaccggatcc gctcgcacgg
     3781 gtctatcgcg tcgacgagac gagcctgacc gccttcggca gcttcgggat cggagctgac
     3841 gatcgcgtcg agtggatgcc gcttccgggt ccgtaaccca cgcgcgcggt gatccgcgcc
     3901 gggaccgcgc gcgaaccccc ggacgtgacc cacgctcgtc gcctcgcccg accgtgcttg
     3961 ccgccgatcg cgatcgctct cctctcggtc gcgatcgtgg cctgtggtga accgcgcgcg
     4021 ccctcgagcc cggcgggctc cgggagcgcg gctcgcgcct cgcctccgcc gtcggcgccg
     4081 tcggcgccgt ccgggggtcc ggaagcgagc gccatctcgt cgagcccaag ctcgccgtcc
     4141 gtcgcgtcga cggctgccga gatcgcccgc ctcgaagctg tcgtcgccgc tgatccgacc
     4201 gatgccggct cccagcggga cctcggcttc gcgctcgccc agcgcgtccg cgagaccgcc
     4261 gacccgtcgc tctacgcccc cgccgaggcg gcgttccggg ccgcccttcg actcaggccc
     4321 gacgacgccc cggcgctcgt cgggatcgcc gggatccagc tcgggaagca cgagttcgcg
     4381 gccggtctcg agaccgcgcg ccgggcgctg gccctctcgc cgtcgctcgt tgcggcgcac
     4441 gcggcccagg tcgatgcgct cgttgagctc ggccggtacg acgaggctga caccgcggcc
     4501 ggggccatgt tcgggctgag ctcggacctc tcgacgctgg cccgggtgtc gtatctcgcc
     4561 gagctccgcg ggcgcctgcc cgcagcgctc acggcgatgc gcgcggccgc ccgggcgccc
     4621 gggctcgccc cggagaacac cgcctacatc gacgccctgc tgggcaacct cctcgtcgcg
     4681 aacggcgatc cggacggtgc cgcgaccgct tacgcgtcgg ccctggtcct cgtcccggag
     4741 cacgcgccgt cgctcgcggg cggagggcgg ttggcggtcg gcgcggggca cctcgacgag
     4801 gcgatcgagc gcttcgagca cgccgcgcgg gtcgtgccgc tgcccgaata cgtgatcgcc
     4861 ctcggcgacg cccagaccgc cgccgggcaa gccgacggcg ccgtacggag ctacaagctc
     4921 gcgcgcgccg agatccagct gttcaaggcc gccggcgtcg tcgtcgacgt cgatcttgcc
     4981 ctcctcgagg ccgaccacgg cgatccggca gcggccctct cctacgccat ggcggccctc
     5041 gcggtcacgc cgacggtgcg cgccgcggac gcggtcgggt gggcgctcca tcgcctcggc
     5101 cgcaaccggg aggcgctggc gcaagtgaag gacgccctcc ggctcggttc gatcgatccg
     5161 atcctgcgct accacgcggg agccatcgag gcgtccctcg gcaaggccgt cgatgcgcgc
     5221 cgggacctga cgcgggcgct cgcgacggac tcgggtttct cggcgaccgg cgccgcggag
     5281 gcgcgacgcc tgctcgcctc gctgaagggc tgactcgagc ctgcccgccg ttggctgcgc
     5341 gcccggtgcg cgctgcggcg ctcgggagca tcgttcccgg ctgtcatccg tcgatctgcg
     5401 ccgcaggcgc gcagtccggc gtcggagcga caggaatccg ccggtccggg ccggatcgca
     5461 cgtcgtcctt cgtcgcgacg cgcgccgggt gcgcagtcgg cgcctgcccg cagtggcgcg
     5521 cgtcgcccat tcatccaccg ggcgttcatc cagaacgccg gacaccgcga acacccctcg
     5581 acagcgcacc ggtgaggagt cgcgacgcac cggacctgca ccttcgatcc agcacccgaa
     5641 gggacgttcg ggttctgccc cagccctgca ccggagaacg atccgtgtcc acacgatcca
     5701 aggcagccgc cctcaccggc gccctgatcc tcgcgaccag ctcggtcggt gggatcctgg
     5761 cgtccagcca tcgcgaggcg ccgctcatcg ccggcgatcc cgcggccgac aacaccgacc
     5821 tctacgcctt cctcgatccg aacgacaaca cgaagctgac gatcatcgcc aactacgtgc
     5881 cgctcgagga gccggccggc ggtccgaact tcttcccgtt cgacccgaac gtccggtacg
     5941 agatcaagat cgacaacacc ggtgacggcg tcgccgacgt gacgtaccgg ttcaagttca
     6001 agacgcgctc gcaggcgacg aacttcgccg gcatcccgac gttcctctac aacgacggcc
     6061 cggtgaccag cctggccgat ccgaacctgc tcgtgaagca gacctacagc atcgagcgca
     6121 acggcgtcgc gatcggcagt ggcctgcgga ccccgccggt caacgtcggc ccgcgctcca
     6181 cgcccgacta cgccatgacg gccgggcagg ccatctacac cctgggcgac ggaatgaagg
     6241 tgttcgccgg ccagcgtgac gacgccttct tcgtcgacct cggctcgatc ttcgatctgg
     6301 ccggactccg caacttcaac gcgctccacg cgatcccgct gcccgcggcg gcgggcgtcg
     6361 acggcgtcgg tgggttcaac accaactcga tcgcgatcga cgttccgctc aatctcctga
     6421 cgaaggatca ccatcagccg accggtccga acgaccccga cgccgtgctc ggcatctggg
     6481 cgacggccag tcgccagcag acccggaagc tcaacgccat cgggacgatg agcttcagcg
     6541 gtccgtgggt ccaggtctcg cgcctcggca acccactgat caacgaggtg atcatccccc
     6601 gcgagaagaa ggactactgg aacggccaga cgccgaacca cgacagccag ttcgcgaagt
     6661 actacaaggc gcccgaggtc acggctgttg ccaacgtcct ctatgacgcc ctcgacacgc
     6721 cggccacgac gaaccgcaac gacctcgtcg cgatcctcct gaccggtctc gacatcccga
     6781 gctcgacggt cgttccgggc ggcctccact acacgcggac cggcacgacc caggccgacc
     6841 tgctccgcgt caacaccggc atcaagccga acgcggccgg cgcctgtgtc ttcggcgtgg
     6901 ccggtggcgg ctctcccagc cgcctcgggg cactcgacgg cgacctgtgc ggcttcccga
     6961 acgggcgccg gctcgccgac gacgtgaccg acatcgagct gcgcgccctc ctcgagggct
     7021 acggcccgac cctcaacgcg gtcctcggcg tcccgaaccg cagcccgaac aacctcatcg
     7081 gcgacggcgt cgacgagaac gacgtgccct tcctcgcggc cttccccttc gaagggaccc
     7141 cgaaccaggg gtatgagcac acccaccacc ggcttgggcc gatcccgatc tgagcaaccc
     7201 gcgctcccgt gacgcgaacg gcccgggccc cgcccgggcc gttcggaatc cccgggggtc
     7261 gagccgggac acgtccgaaa tcgcatgccg ccaacgacga catcctgcac agcgaatcgt
     7321 gcggttcgga cgccgatttc gtcccagcga gccgaaacga gagtgacagt cggcagcggc
     7381 cgatctcgga cgtccgcggg cggttgacag ccctgctcca cctcgcggat catgcattgc
     7441 acatctgtaa cgtgcaaatg tgcgccacga tcccggaacc ccggagcccg cgtgtccgac
     7501 gtccagcagc tgatccaggc gagccgcctc tactacgaac tcggtgagac gcagtcgcgc
     7561 gtcgccgagc tcctcggcgt cacccgaccg caggtctccc ggctgctcaa gcgcgcccgg
     7621 gcggagggga tcgtcgagat ccggatcgtc gatcaggcgt cggt
//
LOCUS       DATAIW010000123         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_155053, whole genome shotgun sequence.
ACCESSION   DATAIW010000123 DATAIW010000000
VERSION     DATAIW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155053"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VHM48_04060"
     CDS             complement(<1..1142)
                     /locus_tag="VHM48_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2754609.1"
                     /translation="MPSARQTSIIGRDDEVRAVQAFVADAGGPPRSLAIAGAAGMGKT
                     LLWRSAVDTARAGGSLVLIARATERELDLPYAALADLIGRLAEAHGAALPDLQRSAIA
                     AVLLQADPNVALDVRTVGTAVVAMLERAVASRPVLIAIDDEPWIDAASRETLRFAIRR
                     LPDRARLLVTRRTPFDDASSGLGDALPADSRTRIELQSLSAAALHDMLRERLGAAPAR
                     PALTRITDVSAGNPFFALELARAATTSGAEPSGFFDPMTIPASLRELMSARLAALGDR
                     ARLVLTAIAALDRPSPATIGLVLTERGEPAQSVDQDLEQLERDRLIVFDDDLRVSHPL
                     ITEIVLDDLGPGRRRLLHRLLAGIVTDEDQVARHLALGTIDPAEPIA"
     gene            1274..1651
                     /locus_tag="VHM48_04065"
     CDS             1274..1651
                     /locus_tag="VHM48_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754610.1"
                     /translation="MRRTNLSLYYLAGYLLPAGTLLLLVPDFATKLLLSNRTYDDAPL
                     RLAGVLLIGLGLLVVQMIRHRLEVLYPTTIVIRLLISATLIGLFVETSDPFFLVVLTV
                     VVIGIALTGISLVLDRRQEMAAA"
     gene            1952..2626
                     /gene="fsa"
                     /locus_tag="VHM48_04070"
     CDS             1952..2626
                     /gene="fsa"
                     /locus_tag="VHM48_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX2754611.1"
                     /translation="MKIFLDTADINEIRQVAKWGVLDGVTTNPSLFAKTSGSTYEEVL
                     KEICTITSGPVSAEVVAEDVEGMLREGRAFAKLAPNIVVKVPMSEEGLEAISTFADEG
                     IKTNCTLIFTANQGLLAAKAGASLLSPFVGRLDDINQDGMIVIRELVDIVTIHDIETE
                     VLAASIRNPLHMTQAALAGAHVATLPFKVLQQMVHHPLTTSGIVQFRADWEKARKALE
                     TANAKA"
     gene            complement(2658..3698)
                     /locus_tag="VHM48_04075"
     CDS             complement(2658..3698)
                     /locus_tag="VHM48_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754612.1"
                     /translation="MAARHRRPAGLRRTLALVVGLVALFSHWSPAPANAAPTWTLNLY
                     TSSAFVYQDPYPTACTAAATMIMLNAIAYRHTGGNAFRWTASRVKNDANTSNTHDMTS
                     ILWFERSHDTLSARGYGSDGHGWRNALNAYGWGSAAMTDPTQWVYDDLGFTSYDAAVH
                     AAVRAIAQYRMPVGILAWAGGHAQVMTGYVVDGDDPALSDAFTVRYIYLSDPLYKDHF
                     VNTKVSNATFKSGTWRIRFQAYRETDSPYDDPYKSGWKRSSVSPAYGPSEWYHRWVIV
                     APIRLPAGVPDPTPTPTPPPNPTPTPDPTATPAPTATPSPVAPEQAGPSDSPSPVAPS
                     EEAPAASPSPTV"
     gene            complement(3822..>4134)
                     /locus_tag="VHM48_04080"
     CDS             complement(3822..>4134)
                     /locus_tag="VHM48_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754613.1"
                     /translation="SLAGMGDGRVTIGGQEIEVHGGRCTIVGSGTLAGSVIALDSAVR
                     NLVRRGASLPATVAAASRNPLAMLGVADRGRLAPGQRADLVELDDELLVRRVMRGGVW
                     R"
BASE COUNT          605 a         1419 c         1501 g          609 t
ORIGIN      
        1 gcgatcggtt cagccggatc gatcgtcccg agggcgaggt ggcgggcgac ctggtcctcg
       61 tccgtgacga tgccggcgag gaggcggtgg agcaggcgcc gccgtccggg cccgagatcg
      121 tcgaggacga tctcggtgat cagcgggtgg ctgacccgga ggtcgtcgtc gaagacgatg
      181 aggcggtccc gctcgagttg ttcgagatcc tggtcgaccg actgggccgg ctcgccgcgc
      241 tcggtcagga cgaggccgat cgtggccggg gatggtcggt cgagggcggc gatggccgtg
      301 aggacgagcc gtgcccggtc gccaagcgcg gcgagccgtg ccgacatcag ctcgcgcagg
      361 ctcgccggga tcgtcatcgg atcgaagaag ccggacggtt cggcccccga agttgtggcc
      421 gctcgcgcca gctcgagggc gaagaacgga ttgcccgccg acacgtcggt gatccgggtg
      481 agggccggcc gggcgggcgc cgccccgagc cgttcgcgca gcatgtcatg gagtgccgca
      541 gcggacaggg actgcagctc gatccgcgtc cggctgtcgg ccggcagcgc gtcgccaagc
      601 ccgctcgacg catcgtcgaa gggcgtccgc cgggtgacga gcaggcgggc ccgatccggc
      661 aggcggcgga tcgcgaagcg cagcgtctcg cgcgatgccg cgtcgatcca tggctcgtcg
      721 tcgatcgcga tcaggacggg cctcgacgcg acggctcgct cgagcatggc cacgaccgcc
      781 gtgccgaccg tccgcacatc gagcgcgacg ttcggatcgg cctggagcag caccgccgcg
      841 atggcggatc gctggaggtc gggaagcgcg gcgccgtggg cctcggccag tcgcccgatg
      901 agatccgcga gggcggcgta gggcagatcg agctcccgct ccgtcgcgcg ggcgatgagc
      961 acgagcgatc cgcccgcccg cgcggtgtcg accgcgctgc gccagaggag ggtcttgccc
     1021 atgcccgcgg ctccggcgat cgccagcgaa cgcgggggac cgcccgcgtc ggcgacgaac
     1081 gcctggacgg cccgcacctc gtcgtcacgg ccgatgatcg aggtctgtct cgcggacggc
     1141 atcccggcga tcgtaccgtc gtcgggcccc cgcgcgaggc tcgccctcaa gcgcccggac
     1201 cgggcaccgg ctcgatgttc gggcaatgca cgaagggcgg accaccgccg gcgcgttgcc
     1261 gagaggaccc acgatgcgtc ggacgaacct gagcctgtac tacctggccg gctatctgct
     1321 cccggccggg acgctgctgc tcctggtccc ggacttcgcg acgaagctcc tcctctcgaa
     1381 ccgaacctac gatgacgcgc cgctccggct tgccggcgtc ctgttgattg gtctcggcct
     1441 gctggtcgtc cagatgatcc ggcaccggct cgaggtgctc tacccgacga cgatcgtcat
     1501 ccgcctcctg atcagcgcaa ccctgatcgg gctcttcgtc gagacgagcg acccgttctt
     1561 cctcgtcgtg ctgacggtcg tcgtgatcgg gatcgccctg accgggatca gcctggtgct
     1621 cgatcggcgc caggagatgg cagcggcctg agccccgcgc ggcgcgctgg cggcgcccga
     1681 ttcccgtctc aacagtcgcc gggggactcg tacggcgggt ttcgagggtg gtggctgccc
     1741 attgggcgtc gatcgggcag gcggccacgc acgtccggcc gggtgatcag tcggtcaacc
     1801 gtcaggcatc tgccgcaaga agtcctggcg cgactgcacg gccaggagtc agccgtcggc
     1861 ccccgcggcc gcgcccggcc cgaatccggg tgccggtggc gtcgggcgta gactcacctc
     1921 gctgaacgac tgacaggagc atcgccccag aatgaagatc ttcctcgaca ccgccgacat
     1981 caacgagatc cgccaggtcg cgaagtgggg cgtcctcgac ggggtcacga cgaacccgag
     2041 cctgttcgcc aagacgagcg gatcgaccta cgaggaggtc ctcaaggaga tctgcacgat
     2101 cacgagcggt cccgtgtccg ccgaggtcgt cgcggaggac gtcgagggga tgctccgcga
     2161 gggccgggcg ttcgccaagc tcgccccgaa catcgtcgtc aaggtcccga tgagcgagga
     2221 gggcctcgag gcgatctcga cgttcgcgga cgaggggatc aagaccaact gcaccctgat
     2281 cttcaccgcc aaccaggggc tgctcgccgc caaggccggc gcgtctctcc tcagcccgtt
     2341 cgtgggccgg ctcgacgaca tcaaccagga cgggatgatc gtcatccgcg agctcgtcga
     2401 catcgtgacg atccacgaca tcgagaccga ggtcctcgcg gcgtcgatcc ggaacccgct
     2461 ccacatgacc caggccgcgc tcgccggcgc gcacgtcgcg acgctcccct tcaaggtcct
     2521 ccagcagatg gtccatcacc cgctcacgac gtcgggcatc gtccagttcc gggccgactg
     2581 ggagaaggcg cgcaaggcgc tcgagacggc caacgccaag gcctagcctc ggtcgtctcg
     2641 ctgagctcgc gagccgatca gacggtcggt gacgggctcg cggccggcgc ctcctccgac
     2701 ggggccacgg gcgacgggct gtcggagggg cccgcctgct ccggcgcgac cggcgacggc
     2761 gtggcggtcg gtgccggtgt ggccgtcgga tccggcgtcg gcgtcgggtt gggtggcggc
     2821 gtcggcgtcg gcgtcgggtc cgggacaccg gccggcaggc ggatcggggc cacgatgacc
     2881 cagcggtggt accactcgga cgggccatag gccggtgaca ccgagctccg cttccagccc
     2941 gacttgtacg ggtcgtcgta cgggctgtcc gtctcgcggt acgcctggaa gcggatccgc
     3001 cacgtcccgg acttgaacgt ggcgttggag accttcgtgt tgacgaagtg atccttgtac
     3061 agcgggtccg acaggtagat gtagcggacg gtgaacgcgt cggacagcgc cggatcgtcg
     3121 ccgtcgacga catagccggt catcacctgg gcatggccgc ccgcccacgc caggatcccg
     3181 accggcatcc ggtactgggc gatcgcgcgc accgcggcgt ggaccgccgc gtcgtagctc
     3241 gtgaacccga gatcgtcgta cacccattgc gtcgggtcgg tcatcgcggc gctgccccag
     3301 ccataggcgt tgagggcgtt ccgccagcca tggccatcgg agccgtagcc gcgggccgac
     3361 aaggtgtcgt ggctccgctc gaaccagagg atcgaggtca tgtcgtgcgt gttcgacgtg
     3421 ttcgcgtcgt tcttgacgcg gctcgccgtc cagcggaatg cgttgccgcc ggtgtgccga
     3481 tacgcgatcg cgttgagcat gatcatcgtt gcggccgccg tgcacgcggt ggggtacggg
     3541 tcctggtaga cgaacgcact cgacgtgtag aggttgagcg tccaggtcgg cgcggcgttg
     3601 gcgggcgccg gcgaccagtg gctgaagagc gcgacgagcc cgacgacgag ggcgagggtg
     3661 cgtcgcaggc cagccgggcg tcggtgtctg gcggccatgc cgggtcgttc ggtctgcact
     3721 ggctcctgac cctagtcgat gacgcggccc tcgccaagta gcgccaaggt catgggacga
     3781 gtatgaaacg tcttcgcaag acggtcgatt ccgaggacac ctcagcgcca gacgccgccc
     3841 cgcatgaccc gtcggacgag gagctcgtcg tcgagctcga cgaggtcggc acgctggccc
     3901 ggcgcgaggc gaccgcggtc cgcgacgccg agcatcgcca gcgggttgcg gctcgcggcg
     3961 gccacggtgg ccggcaggga ggcgcccctc cggacgaggt tccggaccgc gctgtcgagg
     4021 gcgatcacgg atcccgccag cgtgccgctg ccgacgatcg tgcagcggcc gccgtggacc
     4081 tcgatctcct gtccgccgat cgtcacccgc ccgtcgccca tccccgccag ggac
//
LOCUS       DATAIW010000124        11606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_95322, whole genome shotgun sequence.
ACCESSION   DATAIW010000124 DATAIW010000000
VERSION     DATAIW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95322"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VHM48_04085"
     CDS             <1..381
                     /locus_tag="VHM48_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754614.1"
                     /translation="VLVPAAVAAGAALAIGNTLVDVERDRAAAVSSIAISLGPSRASR
                     LGAVLLAAVWVAAVGSAIATGAGWIAVAAITVAGMIPVVAAIGARAAASAARERAWQA
                     EAIGLAVLATVWLAIVLAAGRPLG"
     gene            519..938
                     /locus_tag="VHM48_04090"
     CDS             519..938
                     /locus_tag="VHM48_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754615.1"
                     /translation="MSLATRRLRSLLIALAVLALSATAALAGRTVLSTPQHQGASQNE
                     QAEESDAPDAEESEAPEGSEAPESPDPSGTDAADTTDTTDAATGEHPDNHGKLVSEAA
                     QSQTPDGFDNHGAYVRTIAQANHGHSGDAAKHAGKQH"
     gene            complement(1047..2021)
                     /locus_tag="VHM48_04095"
     CDS             complement(1047..2021)
                     /locus_tag="VHM48_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2754616.1"
                     /translation="MSEDRELSPAIVSLLSSENAGRSTGVKVISIDRIESNPGQPRMV
                     FDADALHELSASIKEHGVLQPILVRPLGENRYQLIAGERRWRATREAGIATIPALVEE
                     IDDDTALEISIIENLQREDLSPLEEATMFDRMVREHGYSIRKLADKLGKDKGYLENRL
                     RLADAPEEIRELVSLRKDTVSHAYELLKVQDPKKRKRLAAQVASGELSLVKLREKIEG
                     RRTRVRDAESPDEEALEAGQASQEPDDAASGWNPSDRVPVTIGDDSLVNARHQLSDAV
                     EDLVAVLRAPDVMRAIPDIDRANLAKYLTIAKLKLENAIALIRTGDSD"
     gene            complement(2047..2817)
                     /locus_tag="VHM48_04100"
     CDS             complement(2047..2817)
                     /locus_tag="VHM48_04100"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2754617.1"
                     /translation="MTQVIAIANQKGGVGKTTTAINLAGALEEEGYRVLCIDMDPQAN
                     LTAGLGINLNTVERSMADVLADGRSTLPEVIRPTESAGIDVAPANIDLASTEGELFTA
                     LGREQILRDALDGQVDVYDYVLIDCPPNLGLLTVNGLVAANSVIIPVQTQYYAMKGLN
                     NLVKVINMIRMKLNRDLRILGLLPTFYDSRTNLARDMLDELRVVGDHHVFASIIRNTV
                     KLGEAPLAGRPITSYAGSSEAARTYRELAREVIDLGQA"
     gene            3088..4635
                     /locus_tag="VHM48_04105"
     CDS             3088..4635
                     /locus_tag="VHM48_04105"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2754618.1"
                     /translation="MPTNEERTARLEAMRQRARVGGGQVRIDQQHARGKLTARERLEL
                     LLDADSFVEMDAFVTARGTDDDGDAILGDGVVTGHGTIDGRNVFVFSQDFTVFGGSLS
                     EAYAAKICKVMDLAMKVGTPIIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVVPQ
                     LSVVMGPCAGGAVYSPAITDFTVMVEGTSYMFVTGPNVVKAVTHEDVDSERLGGAQTH
                     TTRSGVAHIAAPDEASALETTRRILSFLPQNNLADPPRRTTTDSLTRRDAALDRVVPD
                     DPSKPYDMHDVIGPIVDDADFLEIQPGWAQNVIVGFARLGGRSVGIVAQQPSVLAGAL
                     DINASTKAARFVRTCDCFNIPLVTFVDVPGFLPGVDQEDGGIIRHGAKLLFAYCEATV
                     PKLTVITRKAYGGAYDVMSSKHIRGDMNFAWPTAEIAVMGPAGAVNVIHRDAIDAADD
                     ADEERSRLVAEYEERFANPYIAASRGYVDDVILPSETRSRLIRALELLADKRDTNPPK
                     KHGNIPL"
     gene            4695..6260
                     /locus_tag="VHM48_04110"
     CDS             4695..6260
                     /locus_tag="VHM48_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2754619.1"
                     /translation="MLVANRGEIAVRIIRACREMGIATVAVYSDADVDAPHVRLADRA
                     IRLGPPAAVDSYLRGDLIVEAALSTGAEAIHPGYGFLAERASFARATEDAGLVFVGPS
                     STTIELLGDKVAARRTAVAAGIPILPGTLEPFAVDAPDAVEAILVAAERIGYPLLVKA
                     AAGGGGRGMRRVMTADDLPAALIAGADEAAAAFGDGAVYLEREIHPARHIEVQLLGDV
                     EGSVVALGERDCSLQRRHQKLLEEAPAPLLTEAERRDLHETAVKAAMAVGLVNAATAE
                     FLFDTSRRFWFLEINTRLQVEHGVTELVHDIDIVREQLWIAAGYPCSERVLDAAAQAA
                     RPKRHAIEVRLSAEDPARGFAPTPGTIGRWDLPHGPGIRVDTAVASASRIVPDYDPLI
                     AKLMVVDESRDAAIDRLARALDETTIGGIQTTLPFHRFIVADPAFRVADLSVDWVDRH
                     WAAIATERRLEALDIAARAAGAAVADVGSGASPTPLVGGSAATGKDIVGGPAVASAWS
                     RVGRARAIDRWPR"
     gene            6257..6745
                     /locus_tag="VHM48_04115"
     CDS             6257..6745
                     /locus_tag="VHM48_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX2754620.1"
                     /translation="MTRRFRVVPSAALVPGDEPRDIDLDAVDPSGAGIGREPMVFVGP
                     VRPSAAGGRATVEVVVDGWRFELDVEDHARAELRRRATREPDATAASGPTEIRAIIPG
                     RIAAIRVAPGDVVEAGQGLLVVEAMKMQNELRAPVSGTVERVAVEEGQTIDNGDILVV
                     VR"
     gene            6742..8436
                     /locus_tag="VHM48_04120"
     CDS             6742..8436
                     /locus_tag="VHM48_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2754621.1"
                     /translation="MSDERQEKRTEERAVDRWRSTTRARALGSVPERRERFTTSSDLE
                     IADLYTAADLEASGFDPERDLGLPGEPPFTRGVQPTMYRSRFWTMRQYAGFATAEETN
                     QRFRYLLEQGQTGLSVAFDLPTQMGYDSDAPEAEGEVGRVGVPISSLADMEILLDSLP
                     LGDVSTSMTINSTAPILLALYVAAAEKQGVARDRIAGTTQNDILKEYIARGTWIYPPA
                     ASMRLVTDIFEFCAAGLPRWNTVSISGYHMREAGATAAQELAFTLADAIAYCEAAVAR
                     GLAIDDFAPRLSFFFAAWSELFEEVAKFRAARRMWARIIRDRFGSTSARSMACRFHVQ
                     TAGSSLTAQSIDNNVVRTTVQALAAVLGGAQSLHTNAKDEALALPTEASARLALRTQQ
                     ILAHESGVTETPDPLAGSYFVESLTTQLEGAAQAYLDEIDAMGGTLAAIEGGFQQRQI
                     QESAYRVQQQIERGDLVVVGVNKFRDDGAGEVKAALQRIDPEGERRQIERVRRVRAER
                     DPAAWIAALARLEGEARGTANLLPPIIEAVNAYATVGEISDRLRSAFGVHRELITI"
     gene            8460..8927
                     /gene="mce"
                     /locus_tag="VHM48_04125"
     CDS             8460..8927
                     /gene="mce"
                     /locus_tag="VHM48_04125"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2754622.1"
                     /translation="MPSPVDRIPPTIRGVGKIHHVALIVASIDDALRLWRDLLGLPLE
                     TVMDIPKDRVRIAFLAVGESKVELVQPTDDTTGVARFLASKGEGFHHVCFEVTNLSET
                     LLRLEFDGLELIDTAPRRGAEGPVAFIHPRSCHGVLVELIEAPGGPSWTTLGF"
     gene            complement(8924..9778)
                     /locus_tag="VHM48_04130"
     CDS             complement(8924..9778)
                     /locus_tag="VHM48_04130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2754623.1"
                     /translation="MSEARGPGDPAANAPFIAANRAVWDEWTAIHETSAFYDLGGFRQ
                     GGIRLRPYEIEEIGPIEGQTLLHLQCHFGIDTLSWARLGARVTGADFSPAAIELASRL
                     AQELGFPDARFVRSTVDDLPETLDGQFDVVYTSRGVLGWLPDIRRWARVVAHFVRPGG
                     RFYITEIHPVANIFENEGVQPGELDLRYPYWEHREPLSFPVQGSYADPTAPMTATVEH
                     GWDHGLGEIVTALIEAGLEIRSLREYPFVDWRLDFLAEDDDGTWRLPPTVAGELPLFF
                     SILAVKPG"
     gene            complement(9775..10236)
                     /locus_tag="VHM48_04135"
     CDS             complement(9775..10236)
                     /locus_tag="VHM48_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754624.1"
                     /translation="MMDLVAGHEREILLAIAVDDWDGFDDRGRFDAHLALGGGLDPTW
                     LDLFSEAIRVVTGGDGPADFIDARRELDGPGDAGDRVIERVDPAWIDAIARMADHDID
                     AVAGHWIDLVEEELGFLPREEKPWIRKLAGDIVAFARAADHSPDVLFAWSL"
     gene            complement(10248..10784)
                     /locus_tag="VHM48_04140"
     CDS             complement(10248..10784)
                     /locus_tag="VHM48_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2754625.1"
                     /translation="MTIDKIRTLFAYDRWATEKVLAVLDGVEPAIWSATQVVDERGLG
                     GILVHQLGATQRWRHGFLEEGVMPRPEREPLPSIDGLRAAWAVEWPVIDAWLAGLDDA
                     WLARDDEGIGYWQMLAHVVNHGTQHRSEAAAILTQAGCSPGDLDLIFFAEEQAGWIDG
                     KPPRANGVDGAPIPTSEG"
     gene            complement(10812..>11606)
                     /gene="sucD"
                     /locus_tag="VHM48_04145"
     CDS             complement(10812..>11606)
                     /gene="sucD"
                     /locus_tag="VHM48_04145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2754626.1"
                     /translation="YGTNIVAGMTPGKGGQTALDGKVPVFDTVADAVRETGADTSCIF
                     VPAAGAPDAVMEAAAAGVKTIFCITEGIPALDMIPVVEMVRAAGARLIGPNCPGATSP
                     GRAKVGIIPGSIHREGRVGVVSRSGTLTYEAVQAMTDAGIGQSTCIGIGGDPVIGTTF
                     LDILQLFAADDETTAIVLIGEIGGAAEETAAAWAAEHLRDVPKVAFIAGRTAPEGKRM
                     GHAGAIISGGSGTAASKVTALEAAGFRVAGSPTELPGLLRDAGYRA"
BASE COUNT         1756 a         4015 c         4055 g         1780 t
ORIGIN      
        1 gtcctggtgc ccgccgcggt tgccgccggg gccgctctgg cgatcggcaa caccctcgtc
       61 gatgtcgagc gcgatcgggc cgcggcggtc tcctcgatcg ccatctcgct cggtccgtct
      121 cgtgcctcgc gactgggcgc cgtgctcctg gcggcggtct gggtagccgc cgtcggatcg
      181 gccatcgcga ccggcgccgg ttggatcgcc gtcgccgcga tcacggttgc gggcatgatc
      241 ccggtcgttg ccgcgatcgg cgcgcgggcg gcagccagcg ccgcccggga gcgggcctgg
      301 caggccgagg cgatcgggct tgccgtcctc gcgaccgtct ggctggcgat cgtccttgcc
      361 gctggtcgac cgctcggctg aggcggcccg accggcgggt caagcccggg cgatggggcc
      421 gtgcgctcgc gcaccaaccc gggacgaagg tacccctaac cgaccgcggg gcaccgtcgt
      481 tctccttggt gtcatcccac cacggaggaa tcatcatcgt gagtctcgcc acccgccggc
      541 ttcgcagtct cctcattgcg ctcgctgtcc tcgccctctc cgcgaccgcc gccctggctg
      601 gacgcaccgt gctgtcgacc ccgcagcacc agggcgccag ccagaacgag caggccgagg
      661 aaagcgatgc gcccgatgcc gaggagtcgg aggcgccgga ggggtccgag gcccccgagt
      721 cacccgatcc gtcggggacc gacgcggccg acaccaccga caccaccgac gcggcgaccg
      781 gcgagcatcc cgacaaccac ggcaagctgg tcagcgaggc cgcccagagc cagacgcccg
      841 atggcttcga caaccacggc gcatatgtcc ggacgatcgc ccaggccaac cacggtcatt
      901 cgggtgacgc ggccaagcac gccggcaagc agcactgacc gggctgcggg ccgaaccgtc
      961 gggcccgcac gccaagcccg acaagccgaa gccggcgccg cgaggcgccg gcttcttgct
     1021 gcaggtcgac cggagacggg gagtgatcag tcgctgtcgc cggtccggat gagggcaatc
     1081 gcgttctcga gcttgagctt ggcgatcgtc aggtacttcg cgaggttggc ccgatcgatg
     1141 tcggggatcg cgcgcatgac gtccggggcg cgcaggaccg cgaccagatc ctcgacggcg
     1201 tcggacaact ggtgacgggc gttgacgagc gagtcgtcgc cgatcgtgac cgggacccgg
     1261 tccgaggggt tccatccgct cgccgcatcg tcaggttcct gtgaggcctg cccggcttcg
     1321 agggcttcct cgtcgggtga ttcagcatcg cggaccctgg tccgacgacc ctcgatcttc
     1381 tcgcgaagct tgacgagcga gagttcgccg gaggcgacct gcgcggccag ccgcttgcgc
     1441 ttcttcgggt cctggacctt gagcagctcg taggcgtggg agacggtgtc tttgcgcaag
     1501 gacaccagct cgcggatctc ttcgggggcg tcggccagac ggagacgatt ctcgagatag
     1561 cccttgtcct tgcccagctt gtcggcgagc ttgcggatgc tgtagccgtg ctcgcggacc
     1621 atccggtcga acatcgtcgc ttcctcgagc ggcgaaaggt cctcgcgttg gaggttctcg
     1681 atgatcgaga tctccagcgc cgtgtcgtcg tcgatctcct caacgagggc ggggattgtc
     1741 gcgatgcccg cttcgcgcgt ggcgcgccag cggcgctcgc ccgcgatgag ctggtagcgg
     1801 ttttcgccga ggggacggac gaggatcggc tggaggacgc cgtgttcctt gatcgacgcg
     1861 gacagctcgt ggagcgcgtc cgcgtcgaag accatccgag gctgacccgg attcgactcg
     1921 atccggtcga tcgagatgac cttgacgccg gtgctccgtc cggcgttctc ggacgagagc
     1981 aggctgacga tcgcgggcga gagctcgcga tcctctgaca atcgacgggc ggcgacggcg
     2041 cggaagtcag gcctggccaa gatcaatgac ctcccgggca agctcgcgat acgtacgagc
     2101 ggcttcggat gagccggcat acgacgtgat cggtcgtccg gcgaggggcg cctcaccgag
     2161 cttcaccgtg ttccggatga tgctggcgaa cacatggtga tcgcccacga cgcgaagctc
     2221 gtcgagcatg tcgcgggcga ggttcgtccg gctgtcgtag aaggtcggga ggagaccgag
     2281 gatccggagg tcacggttca acttcatccg gatcatgttg atcaccttga cgaggttgtt
     2341 cagtcccttc atcgcgtagt actgggtctg gaccgggatg atcacgctgt tcgccgcgac
     2401 caggccgttg acggtcagga ggccgaggtt cggtgggcaa tcgatgagga cgtagtcgta
     2461 gacgtcgacc tggccgtcga gggcgtcgcg gagaatctgc tcgcgaccca acgccgtgaa
     2521 cagctcaccc tcggtcgacg ccaggtcgat gtttgcggga gccacgtcga tgccggcgct
     2581 ctccgtgggt cggatcacct cgggcagcgt cgagcgtcca tcggcgagga cgtcggccat
     2641 cgatctctcg acggtgttga gattgatgcc gaggcccgcg gtcagattgg cctgcggatc
     2701 catgtcgatg cagaggacgc gatagccctc ttcctcgaga gcgccggcga gattgatcgc
     2761 cgtggtcgtc ttgccgaccc cgcccttctg attcgcgatc gcgatcacct gcgtcaccgg
     2821 ggcatcacct cgaatcgtgt cagcctcgcg acgggtcgac gcgggggcgg ggaagaccgt
     2881 catgctaccc cagcgatccg gtgggcgatc gttgaggtta tccacagcat ccgcgagttg
     2941 ttcaccgttt ctgtggatat gtcgtggttc gcttgttgcc cttcgttgac gggtcggcgc
     3001 gccaaatggc cctcgccgca cctcctatga tggatcccgg tgttgctgcg acacctgtcc
     3061 ttgcgcaaag acacgacagg agtgcatgtg ccgacgaacg aggaacggac cgctcggctc
     3121 gaggccatgc gccagcgggc gcgagtgggg ggcggacagg tccgcatcga tcagcagcac
     3181 gcccgcggca agctgacggc gcgcgaacga ctcgagctgc tgctcgacgc ggattcgttc
     3241 gtcgagatgg acgcgttcgt gacggcccgc gggaccgacg acgacggcga cgcgatcctg
     3301 ggcgacgggg tggtgaccgg gcacggcacg atcgacggcc ggaacgtctt cgtcttcagc
     3361 caggacttca cggtcttcgg tggttcgctg tccgaggcgt acgcggcaaa gatctgcaag
     3421 gtcatggacc tcgccatgaa ggtcggcacg ccgatcatcg gtctcaacga ttcgggcgga
     3481 gcgcgaatcc aggaaggcgt tgtgtccctt ggcggctatg ccgacatctt ccttcgcaac
     3541 accctcgctt cgggcgtggt tccgcagctg tccgtcgtca tgggaccgtg cgccggggga
     3601 gcggtctatt cgccggcgat caccgacttc acggtgatgg tcgaaggaac gagctacatg
     3661 ttcgtcacgg gacccaacgt cgtgaaggcc gtcacccacg aagacgtcga ctccgagcgg
     3721 ttgggcggcg cgcagacgca cacgactcgc agtggcgtgg cccacatcgc tgcgcccgat
     3781 gaggccagcg cgctcgagac cacgcgccgg atcctgtcgt tccttccgca gaacaatctc
     3841 gcggacccac cccgccggac cacgacggat tcgttgacgc ggcgcgacgc ggcgctcgat
     3901 cgcgtcgtcc ccgacgatcc gtccaagccg tatgacatgc acgacgtcat cggcccgatc
     3961 gtggacgacg cggactttct cgagatccag ccgggctggg cacagaacgt gatcgtcggg
     4021 tttgcgcggc tgggcggccg gagcgtcggg atcgtggctc agcagccgtc cgtccttgcc
     4081 ggcgcgctgg acatcaacgc ctcgacgaag gccgcccggt tcgtccggac gtgtgactgt
     4141 ttcaacatcc cgctggtcac gttcgtcgac gtccccggct tcctgcccgg tgtcgaccag
     4201 gaggatggcg gaatcattcg tcacggggcc aagctcttgt tcgcctactg cgaagcgacg
     4261 gtgccgaagc tgaccgtcat cactcgcaag gcctacggcg gtgcctatga cgtcatgagc
     4321 agcaagcaca tccggggcga catgaacttc gcgtggccga ccgcggagat cgccgtcatg
     4381 ggcccggccg gcgcagtgaa cgtcatccat cgcgacgcga tcgacgccgc tgacgatgcc
     4441 gacgaggagc gatcccgtct cgtcgccgag tatgaggagc ggttcgccaa cccgtacatc
     4501 gccgcatccc gcggctatgt cgacgacgtc atcctcccgt ccgagacgcg ttcccgcctg
     4561 attcgcgcac tcgaactcct cgccgacaag cgcgacacga acccaccaaa gaaacacggg
     4621 aacattcccc tgtgacgtcg gttgcgaccg cgtctttgcg caaagacagg ggagggcagc
     4681 gaccgttcga ccgggtgctc gtcgccaacc gcggcgagat cgcggtgcgc atcatccgcg
     4741 cctgccgcga gatggggatc gcgacggtcg cggtctacag tgacgccgac gtcgatgcgc
     4801 ctcatgtccg actggccgac cgagcgatcc gcctgggtcc gcctgctgcg gtcgactcct
     4861 acctgcgggg tgacctgatc gtcgaggctg cgctgtcgac gggggccgaa gccatccatc
     4921 ccgggtatgg gttcctggcg gaacgtgcct cgttcgcgcg cgcgaccgag gacgccgggc
     4981 tcgtgttcgt cggtccgtcg agcacaacca tcgagctgct cggtgacaag gtggccgctc
     5041 gccggacagc ggtggccgcg ggtatcccca tcctgcccgg gacgctggag ccgttcgcgg
     5101 tcgacgcgcc cgacgcagtg gaggcgatcc tcgtggccgc cgagcggatc ggctatccgc
     5161 tgctcgtgaa ggccgcggcg ggcggaggcg gccgggggat gcggcgcgtc atgaccgccg
     5221 acgacctgcc ggcggcgctg atcgccggcg ccgacgaggc cgcggcggca ttcggtgacg
     5281 gagccgtcta tctcgagcgc gagatccatc ccgcccggca catcgaggtc cagctgctcg
     5341 gcgacgtcga aggctcggtc gtcgcgctcg gggagcgcga ctgctccctc cagcgtcgcc
     5401 accagaagct gctcgaggaa gcccctgcgc cgctgctcac cgaggcggaa cggcgcgatc
     5461 tccacgagac ggccgtcaag gccgcgatgg ccgtcggcct cgtcaacgcc gcgaccgcgg
     5521 aattcctgtt cgacacgagt cgccggttct ggttcctcga gatcaacact cgcctccagg
     5581 tcgagcacgg cgtcaccgag ctcgtccatg acatcgacat cgtccgcgag cagctgtgga
     5641 tcgcggccgg atacccctgc tccgagcggg tgctcgacgc ggccgcgcag gcggcgcgcc
     5701 cgaagcgcca tgcgatcgag gtccgactgt cggccgagga tcccgcccgt gggttcgccc
     5761 cgacccccgg gacgatcggg cgctgggacc tgccgcacgg acccgggatc cgggtcgaca
     5821 ccgcggtcgc gtcggcaagc cggatcgtgc cggactacga tcccctgatc gcgaagctga
     5881 tggtggtcga cgagagccgc gatgcggcca tcgatcggct ggcccgggct ctggacgaga
     5941 cgacgatcgg cggcatccag acgacgttgc ccttccatcg gttcatcgtc gccgaccccg
     6001 cgttccgggt ggccgacctg tcggtcgact gggtcgaccg acactgggcg gcgattgcca
     6061 ccgagcgccg gctcgaggcc ctcgacatcg ccgcccgcgc ggcgggtgcg gcggtcgcgg
     6121 acgtcggctc gggggcatca ccgacgcccc tcgtcggtgg ctccgcagcg accggaaagg
     6181 acatcgtggg cggtccggcc gtggcgtcgg catggtccag ggtcggccgg gcccgtgcga
     6241 tcgaccggtg gccccgatga ctcgccggtt ccgggtggtg ccgtcggcgg ccctggtgcc
     6301 tggcgacgag ccacgcgaca tcgatctcga cgcggtcgat ccgtcgggcg ccgggatcgg
     6361 gcgcgagccc atggtcttcg tcggtccggt acgcccgtcg gcggcgggcg gtcgggcgac
     6421 ggtcgaggtc gtcgtcgacg gctggcggtt cgagctcgat gtcgaggacc acgctcgggc
     6481 cgagctgcgc cggcgggcga cccgggagcc cgatgcgacc gcggcaagcg gacccaccga
     6541 gatccgggcg atcatccccg ggcggatcgc ggcgatccgg gtcgcgccgg gtgatgtggt
     6601 cgaggcgggc cagggcctcc tcgtcgtgga ggcgatgaag atgcagaatg aactccgggc
     6661 gccggtctcg ggaacggtcg aacgggttgc cgtcgaggag ggccagacga tcgacaacgg
     6721 cgacatcctc gtggtcgtcc gatgagcgac gagcgacagg agaagcggac ggaggagcga
     6781 gccgttgacc gctggcggtc gacgacccgg gcccgcgccc tcggctccgt gccggagcgc
     6841 cgcgagcggt tcacgaccag ctccgatctc gagatcgccg acctctacac ggcggccgac
     6901 ctcgaggcca gcgggttcga cccggagcgt gacctcggcc tgcccggcga gccgcccttc
     6961 acccgtggcg tccagccgac gatgtaccgc agccgcttct ggacgatgcg ccagtacgcc
     7021 ggcttcgcca ccgccgagga gacgaaccag cgcttccgct acctcctcga gcagggccag
     7081 accgggctgt ccgtggcgtt cgacctgccg acccagatgg gctacgactc cgacgccccg
     7141 gaggcggagg gcgaggtcgg ccgggtcggc gtcccgatct cgagcctggc ggacatggag
     7201 atcctcctcg acagcctgcc gctcggcgac gtcagcacct cgatgaccat caactcgacg
     7261 gcgccgatcc tcctcgccct gtacgtcgcc gcggccgaga agcagggagt cgcgcgggac
     7321 cggattgcgg gcacgaccca gaacgacatc ctcaaggagt acatcgcccg cgggacgtgg
     7381 atctacccgc ccgcggcgtc gatgcggctc gtgaccgaca tcttcgagtt ctgcgcggcc
     7441 gggctgccgc gctggaacac ggtgtcgatc tcgggctacc acatgcggga ggcgggagcg
     7501 acggccgccc aggagctggc gttcaccctc gccgacgcga tcgcctactg cgaggctgcg
     7561 gtcgcccgcg ggcttgcgat cgacgacttc gcgccccgcc tcagcttctt cttcgccgcc
     7621 tggtcggagc tgttcgagga agtcgccaag ttccgggcgg cccgccggat gtgggcccgg
     7681 atcatccgcg accgcttcgg ctcgaccagc gcccgctcga tggcctgccg gttccatgtc
     7741 cagacggccg gttcgagcct gaccgcccag tcgatcgaca acaatgtcgt ccggacgacg
     7801 gtccaggccc tcgcggccgt cctcggcgga gcgcagagcc tccacacgaa tgcgaaggac
     7861 gaggccctcg ccctgccgac cgaggcatcg gcccggctcg ccctccggac ccagcagatc
     7921 ctggcccacg agagcggggt gaccgagacg ccggacccgc tggccggctc gtacttcgtc
     7981 gagagcctga cgacccagct ggagggtgcc gcccaggcct acctcgacga gatcgacgca
     8041 atgggcggga cgctcgccgc gatcgagggc gggttccagc agcgccagat ccaggagtcg
     8101 gcctatcgcg tccagcagca gatcgagcgg ggcgacctcg tcgtcgtcgg ggtcaacaag
     8161 ttccgcgacg acggggcagg ggaggtgaag gcggccctcc agcggatcga cccggagggc
     8221 gagcgacgcc agatcgagcg ggtccgccgg gtccgcgccg agcgcgatcc tgccgcctgg
     8281 atcgcggccc tcgcccggct cgagggcgag gcccgtggca cggccaacct cctgccgccg
     8341 atcatcgagg cggtcaacgc ctacgccaca gtcggcgaaa tcagcgaccg gttgcgatcc
     8401 gcgttcggcg tccatcgcga gctgatcacg atctgagcca cgagcccgag ggagtccaga
     8461 tgccgtcacc cgtcgaccga attcccccaa ccatccgcgg cgtcggcaag atccatcatg
     8521 tcgcgttgat cgtggcctcg attgacgacg cgctcaggct ctggcgcgac ctcctcggcc
     8581 tcccgctcga gacggtcatg gacatcccga aggatcgcgt ccggatcgcc ttcctcgcgg
     8641 tcggcgagtc gaaggtcgag ctcgtccagc cgaccgacga cacgaccggg gtcgcccggt
     8701 tcctcgcgag caagggcgag ggcttccacc atgtctgctt cgaggtcacg aacctgtccg
     8761 agacgctcct ccgactcgag ttcgacggcc tcgagctgat cgacacggca ccgcgtcggg
     8821 gcgccgaggg cccggtcgcc ttcatccacc cccggtcgtg ccacggcgtc ctggtggagc
     8881 tgatcgaggc gcccggcggc ccgtcatgga ccacgctcgg gttctagccc ggcttgaccg
     8941 ccaggatcga gaagaagagc ggcagctcgc cggcgacggt cggcggcagc cgccacgtcc
     9001 cgtcgtcatc ctcggcgagg aagtccagcc gccagtcgac gaacgggtac tcgcggagcg
     9061 agcggatctc gaggcccgcc tcgatcagcg cggtgacgat ctcaccaagc ccgtggtccc
     9121 agccgtgctc gacggtggca gtcatcgggg cggtcgggtc ggcgtacgag ccctggaccg
     9181 ggaacgacaa cggctcgcgg tgctcccagt acgggtagcg gaggtccagc tcacccggct
     9241 gcacgccctc gttctcgaag atgttcgcca cagggtgaat ctcggtgatg tagaagcgcc
     9301 cgcccggcct gacgaagtgg gcgacgaccc gggcccagcg gcggatgtcg ggcagccagc
     9361 cgagcacgcc gcgcgaggtg tagacgacgt cgaactggcc gtcgagggtc tcgggcaggt
     9421 cgtcgacggt cgatcggacg aagcgggcgt ccgggaagcc cagctcctgc gccagccggg
     9481 acgcgagctc gatcgccgcc gggctgaagt cggcgccggt gacccgcgcc cccagccggg
     9541 cccatgacag ggtgtcgatc ccgaagtggc actggaggtg gaggagggtc tgcccctcga
     9601 tcggaccgat ctcctcgatc tcgtacggtc ggagccggat gccgccctgg cggaacccgc
     9661 cgaggtcgta gaaggcggag gtctcgtgga tcgcggtcca ctcgtcccac acggcccggt
     9721 tggcggcgat gaacggagcg ttcgcggccg gatcgcccgg cccgcgggcc tcgctcacag
     9781 ggaccaggcg aacaggacgt ccggcgagtg atcggcggct cgggcgaacg cgacgatgtc
     9841 gcccgcgagc ttgcggatcc agggcttttc ctcacgtggg aggaacccga gctcttcctc
     9901 gacgaggtcg atccagtggc cggcgaccgc gtcgatgtcg tggtcggcca tccgcgcgat
     9961 cgcgtcgatc caggccggat cgacccgctc gatgactcga tcacccgcgt ctccgggtcc
    10021 gtcgagctca cgccgggcgt cgatgaagtc ggccggcccg tcgccgccgg tgactacccg
    10081 gatggcttcc gagaagaggt cgagccacgt cggatcgagc ccgccgccga gggcgagatg
    10141 ggcgtcgaac cggccgcgat cgtcgaagcc gtcccagtcg tcgaccgcga tcgcgagcag
    10201 gatctccctc tcgtgaccgg cgaccaggtc catcaacgcg cccatcatca gccctccgag
    10261 gtcggaatcg gagctccgtc gacgccattc gcccgaggag gtttgccgtc gatccatccg
    10321 gcctgttcct cggcgaagaa gatcaggtcc aggtcgccgg gcgagcaacc ggcctgggtc
    10381 agaatggccg cggcctccga gcggtgctgg gtcccgtgat tgacgacgtg ggcgagcatc
    10441 tgccaatacc cgatgccctc gtcgtcgcgg gccagccagg cgtcgtcgag tccggcgagc
    10501 cacgcgtcga tcacgggcca ttcgacggcc caggcggccc gcagcccgtc gatcgacggc
    10561 aggggctccc gctcgggccg cggcatgacg ccttcctcga ggaacccgtg gcgccagcgc
    10621 tgggtcgcgc cgagctggtg gacgaggatc ccgccgagcc cgcgctcgtc gacgacttgc
    10681 gtcgcagacc agatcgccgg ttcgacaccg tcgagcaccg cgaggacctt ctcggtcgcc
    10741 cagcggtcgt aggcgaagag cgtgcggatc ttgtcgatgg tcatggtcgt ccctgtcttc
    10801 cgcggacccg atcaggcgcg atagccggca tcgcgcagga gccccggcag ctccgtcggc
    10861 gagcccgcga cccggaagcc ggcggcctcg agcgccgtca ccttcgaagc cgccgtgcct
    10921 gagccacccg agatgatcgc gccggcgtgg cccatccgct tgccctcggg agcggtccgg
    10981 ccggcgatga acgcgacttt cgggacgtcg cggagatgct cggcggccca cgccgcggcg
    11041 gtctcctcgg cggcgccgcc gatctcgccg atcaggacga tggcagtcgt ctcatcgtcg
    11101 gccgcgaaca gctggaggat gtcgaggaag gtcgtcccga tcacggggtc gccgccgatc
    11161 ccgatgcagg tcgactggcc gatcccggcg tcggtcatcg cctggaccgc ctcatacgtg
    11221 agcgtcccgg agcgggagac gaccccgacc cgaccctcgc gatggatcga gcccgggatg
    11281 atcccgacct tggcccgacc cggcgatgtc gcgccggggc agttcgggcc gatcaggcgg
    11341 gcgccggccg cccggaccat ctcgaccacc gggatcatgt cgagcgccgg gatgccctcc
    11401 gtgatgcaga agatggtctt cacgccggcc gcggccgcct ccatcaccgc atcgggggct
    11461 ccggccgccg ggacgaagat gcaggaggtg tccgcgccgg tctcgcggac cgcgtcagcg
    11521 actgtgtcga agaccgggac cttgccatcg agcgccgtct gcccgccctt gcccggcgtc
    11581 atcccggcca cgatgttggt cccgta
//
LOCUS       DATAIW010000125         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_5672, whole genome shotgun sequence.
ACCESSION   DATAIW010000125 DATAIW010000000
VERSION     DATAIW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5672"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..726)
                     /locus_tag="VHM48_04150"
     CDS             complement(109..726)
                     /locus_tag="VHM48_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754627.1"
                     /translation="MTWLPFDPALQHGIQGRRHELFRLAAPVFRAHGYRGATIKALAH
                     ACHLSPAALYHYFPSKAAFATFLIDDDRLDWDSTRIDPDIDPLVQLRMSVDLVIEVLP
                     TYLLAIDLAREVGRPLVQPELARFFGQGEAIFGRVLAAAAPSLVPDEARELARRVLAI
                     IVAGPTTGLAADPASVRATTIDLLRLHLVRVGVDADRFDEAMTRR"
     gene            complement(1792..2241)
                     /locus_tag="VHM48_04155"
     CDS             complement(1792..2241)
                     /locus_tag="VHM48_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754628.1"
                     /translation="MTRLVDAFGSLSAIRGQAVLDIACGSSSSRSPVTGLQTSEFEPW
                     MCRLLLELGAHPVGLDLGDLADEAFEHHRVDLGVPGALDFLATESFDAIHESRLFGSP
                     EFRSAHGPATRRIRREIHREERRLMRPGGILIHSDGRSGPGAQEHAL"
     gene            2616..2936
                     /locus_tag="VHM48_04160"
     CDS             2616..2936
                     /locus_tag="VHM48_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754629.1"
                     /translation="MIIVRMVFQASYGKGGELAVAMAASAKQVIVEMGGDHRWKVMTD
                     LSGPFDTVVQEIEVASLAEWESMRSRLFASAAFREAMAATMAAARALTVSGRTEYWTI
                     EAKG"
     gene            3269..>3538
                     /locus_tag="VHM48_04165"
     CDS             3269..>3538
                     /locus_tag="VHM48_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX2754630.1"
                     /translation="MGLIHRLARVLLSGIFISGGMDVLRDPGPRAVIAGPFLERVRPL
                     APALPSDPVLVVRANAAFQVAAGATLAVGILPRLSALALAGSLVPT"
BASE COUNT          539 a         1194 c         1263 g          542 t
ORIGIN      
        1 ttcgacatac cggagaccgt agccgccggg ctcccgaatc cggtggacga ttcgttcgat
       61 tcgggtggct cgccgctcct ggccccttgc ggccgcgggt cgcgggcgtc accgtcgggt
      121 catcgcctcg tcgaaacgat ctgcgtctac tccgacccgg acgaggtgga gccggaggag
      181 gtcgatggtc gtggcacgga cggaggccgg atcggcagcg agcccggtcg tcgggccggc
      241 gacgatgatg gccaggaccc gacgggcaag ctcccgggct tcgtctggca cgagactcgg
      301 cgcggccgca gcgaggaccc gcccgaagat ggcctcgccc tgcccgaaga accgggcgag
      361 ctcaggttgg accagcggac gtccgacctc gcgcgcgagg tcgatggcca gaaggtacgt
      421 gggcagcacc tcgatcacga gatcgacgct catccggagc tggacgagcg ggtcgatgtc
      481 gggatcgatg cgggtcgagt cccagtcgag ccgatcgtca tcgatgagga acgttgcgaa
      541 cgccgccttg gacgggaagt agtggtagag cgcggcgggg ctcaggtggc aggcgtgggc
      601 gagcgccttg atcgtcgcgc cgcgatagcc gtgtgcgcgg aagaccggtg cggccaggcg
      661 aaagagctca tgacggcggc cctggatacc gtgctggagc gccgggtcga acggaagcca
      721 ggtcatggcg cggcgatcgc cgccggcgcg ggcgctggct caagtgaagg gccgggcatc
      781 tggcacagtg tgcgcttcag gcaggcgcgc ttggtcaaac gccgcgtctg atgccgccga
      841 tctggttcta cgacctcatg tagcgctggc gcgcaccgtg ggagatgggc gctcgcttgg
      901 agctcgtcgc actcgtccaa tgcgcgtact cagcccggac ggccgagggc ccgccccaaa
      961 cgaggtcctt tcgagcgaat gaggaaccgg ccgcgacgga tgacacatcc atcgactcag
     1021 ccacccgcgt cgcgttgtca ccccagggac gccgagcggt cagtgcgcga ccagcgggac
     1081 gatccgaacg tcgactatcg tctgcccctc cgggcgcgca acaggatggc gcccaagatc
     1141 gcgattgcga tcacgatccc tgccgcgaca aacagggcag catcggcgtc gggcgtgacg
     1201 ccgttgccgg cagatgaggg cgtcaccacg gtctgcgtcg ggcggctggc ggtcgacacg
     1261 ggagagaccg ccgcgaccgg tggtggagtc cgactcgtcg tcgggctcgc ggtcgatccc
     1321 gccggcgggc tcggcgccgg cgtgggtccg ggggtcggcg cgggggtcgg ccacacggca
     1381 gccaacccgg gcggcgagat ccgtggccag atcgtggcaa agggcaatcg cgccggctcc
     1441 cactccatcg ccggcggcca gtgcgaccgt cccgccgacg gcatcgccga ctgccattgc
     1501 gaccgtcccg ccgacctcga cgctgccagc cgagccggcc tcgggtggct ccgccgtccc
     1561 ggcggtcatc gggatcctgg ccttcctgat ggtcgtgtca gtcgccagcc gccgcttcgc
     1621 tctgcgtcgg ggccgctgat agcgcgaccg cttaaccttt gggcggacgg accattggtc
     1681 cgtccgcctg cctgtcactg atccgtcagc ctcagtgttg ccgcccgaac gatcgcggac
     1741 aacgccttcg gagccagacg tcggccgcca agcggtgcgc ccgcgcggcc ttcagagcgc
     1801 atgctcttgt gccccagggc cggaccggcc gtcgctgtgg atcaggatcc cgcccggccg
     1861 catgaggcgg cgctcctcgc ggtggatctc gcgacgaatg cgtcgggtgg cgggcccgtg
     1921 cgcggaacga aactcgggtg aaccaaagag tcggctttca tggatggcat cgaacgattc
     1981 ggtcgccaga aagtcgaggg cgccggggac gcccaggtcg acccgatgat gctcgaacgc
     2041 ctcgtcggcg aggtctccga ggtcgagccc gaccgggtgc gccccgagct ccaggagcaa
     2101 ccggcacatc cacggctcga actccgaggt ctgcaggccc gtgactggcg accggctgga
     2161 gctcgaccca caggcgatat cgaggacggc ctggccgcga atcgccgaca gcgagccgaa
     2221 ggcatccact aggcgggtca cgaactccgt catgatcgct acgaggttcg gctccgccgc
     2281 gatcttcgat tcgagacgat gttgggcatc cagcttcccg agggcgtggt caaggagcgc
     2341 tcggtccgag cgtgtccgac cgagcgcggc caggcgtcga agagcgtcag acgtcgtcgc
     2401 gggtgctccg gtgtcggcgg ggaaacgtca ccgccattgc caagccggca tcgtagcgcg
     2461 cggttgggac agccctttgt ccgggcactt ctcgcggatc ttcggcgacc cgcaggtggc
     2521 ttcttgtcgc cgttcggcgg ccagcccata ctcgccgtct ccgagagggt cggcgtccca
     2581 gccaaggctc tcctggcgtc cccgaaggag acgagatgat catcgtgcga atggtcttcc
     2641 aggcctcgta cggcaagggt ggcgagctgg cggtcgcgat ggcggccagc gcgaagcagg
     2701 tcatcgtgga gatgggcggc gaccaccggt ggaaggtaat gacggacctt tccggcccgt
     2761 tcgacacggt cgtccaggag atcgaggtgg cgagcctggc cgagtgggag agcatgcgat
     2821 cccgcctctt cgcgtcggcg gcgttccgcg aggcgatggc ggccacaatg gcggccgcgc
     2881 gcgccttgac cgtctccggg cgcacggagt actggacgat cgaagccaag ggctaggcac
     2941 cctgtccgac cgcccacccc tgctgcgggt ttccctcgtt ctcgcgacgg gaacctggcc
     3001 tccctgaccc atggctcttc gccacggact cctgctgatc cccgtcacga acgttccgac
     3061 ctccgttcat cgggcctcgg agatcgcgta ggccagcccc acagggacct ttacgttacc
     3121 ggcgcgtggg gaaaggtgac gtccgaggct tcgtgagtcg atccggcgag tagagtcagc
     3181 ctgacgcacg ctatggggtg gcggatccga tagcttgcct gggccgatgt ccgcagcgca
     3241 cggccattga cgaccggagg agcgaccgat gggactgatc cacagactgg cccgagtcct
     3301 gctctcgggg atcttcatca gcggtgggat ggacgtcctg cgcgaccccg gaccccgcgc
     3361 cgtcatcgcc gggccgttcc tcgagcgggt ccgaccgctc gccccggcac tgccgagcga
     3421 cccggtcctc gtcgtccggg cgaacgcggc gttccaagtg gccgccggag cgacgctcgc
     3481 ggtcgggatc ctgccccggc tgtccgcact cgcactggcc gggtcgctcg tcccgacc
//
LOCUS       DATAIW010000126         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_35913, whole genome shotgun sequence.
ACCESSION   DATAIW010000126 DATAIW010000000
VERSION     DATAIW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35913"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /gene="gltX"
                     /locus_tag="VHM48_04170"
     CDS             <1..1480
                     /gene="gltX"
                     /locus_tag="VHM48_04170"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2754631.1"
                     /translation="RIAPSPTGPLHIGTARTALFNFLFARHVGGTFLFRLEDTDVARS
                     TIAFERDIIEGLHWLGITWDEGPDVAGGGDIGPYAPYRQMLRLGTYKEASDRLLAADL
                     AYPCFCTPDELEADRKAQEAAKLPPRYVGRCANLTADERVARLAGTGRAAAIRFRVRP
                     GVVGWNDMIRDRIEIDTTNLGGDFVIVRGDGTPLYHFTVVIDDASMAISHVIRGEDHV
                     SNTPKHILLFEALGHAIPVFGHLPLILNPDGTKMSKRKSQTAVDDYRAEGFIREGLMN
                     YFAYLGWSPGTEEDVLSVDEVIERFDIDKVQKGGARFDRGRLEWLNGQWIRKLEPDDL
                     VDRLRPFLQAALDDGRIDRVPADDEVRSLLPVVQERLPTLAAVVDLVGFLWTDDLGLD
                     PALVVPKRWDAATTRVALAAAHDILAGHDAVTWEADELEPPLRELVEARGWKAGDLFM
                     AIRVATTGRTATPPLFDTLVALGRDRTLARLDAAIAALTPLG"
     gene            1675..>2246
                     /locus_tag="VHM48_04175"
     CDS             1675..>2246
                     /locus_tag="VHM48_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2754632.1"
                     /translation="MARTILVVEDDATLRETLADALEVEGFRVIAAADGRDALARFRA
                     DKPDLVLLDLMLPELSGIEVCRIIRAESGVPIVMLTAKDSELDKVVGLELGADDYVTK
                     PFSLRELSARIRALFRRSEQVAAPDAAGPAVVDLGRVQVDLAGHRLLRDGEEVAIKPK
                     AFELLAFLLRHPGQAFTRDQLLQHVWGYDYA"
BASE COUNT          327 a          816 c          768 g          335 t
ORIGIN      
        1 ccggatcgca ccgagcccga ccggccctct tcacatcggt acggcccgga cggcgctgtt
       61 caacttcctg ttcgcccgcc acgtcggggg gaccttcctg ttccggctcg aggacaccga
      121 cgtcgcccgc tcgaccatcg ccttcgagcg ggacatcatc gagggcctcc attggctcgg
      181 gatcacctgg gacgagggcc ccgacgtggc cggcgggggc gacatcgggc cgtacgcgcc
      241 gtaccgccag atgctccgcc tcggcacgta caaggaggcg tccgaccgcc tcctcgccgc
      301 cgatctcgcg tatccctgct tctgcacgcc cgatgagctc gaggcggacc gcaaggccca
      361 ggaggcggcg aagctgccgc cgcgctacgt cggacgctgt gcgaatctca ccgccgacga
      421 gcgcgtcgcc cgcctggccg ggaccggacg cgcggcggcg atccggttcc gggtccggcc
      481 aggcgtcgtg ggctggaacg acatgatccg cgaccggatc gagatcgaca cgaccaacct
      541 cggcggcgac ttcgtcatcg tccgcggcga cgggacgccg ctgtatcact tcaccgtggt
      601 catcgacgac gcgtcgatgg cgatcagcca cgtcatccgc ggcgaggatc acgtcagcaa
      661 caccccgaag cacatcctgc tgttcgaggc gctcggccac gcgatcccgg tcttcggcca
      721 cctgccgctc atcctcaacc cggacgggac gaagatgagc aagcgcaaga gccagaccgc
      781 ggtcgacgac taccgcgccg agggcttcat ccgcgaaggc ctgatgaact acttcgcgta
      841 cctcggctgg agccctggca ccgaggagga cgtcctctcg gtcgacgagg tcatcgagcg
      901 gttcgacatc gacaaggttc agaagggcgg ggcgcggttc gatcgcggcc gactcgagtg
      961 gctgaatggc cagtggatcc gcaagctcga gccggacgac ctcgtcgacc gccttcggcc
     1021 gttccttcag gccgccctcg acgacggccg gatcgaccgc gtgccagccg atgacgaggt
     1081 ccggtccctg ctgccggtgg tccaggagcg gttgccgacg ctcgccgcgg tggtcgacct
     1141 cgtcgggttc ctctggaccg acgatctcgg gctcgatccg gcgcttgtcg tgccgaagcg
     1201 ctgggatgcg gcgacgaccc gggtcgcgct ggccgcggcg cacgacatcc tggccggcca
     1261 cgacgcggtg acgtgggagg ccgacgagct ggagccgccg ctccgcgagc tcgtcgaggc
     1321 gcgcggctgg aaggccggcg acctgttcat ggcgatccgg gtggcgacga ccggccggac
     1381 cgcgacgccg ccgctgttcg acacgctggt cgcgctcggt cgggatcgga cgcttgcgag
     1441 gctcgacgcg gcgattgcgg cgctgacccc gctcggctga gcgacccgtc gcgggcgccg
     1501 cgtgctcgcg agtccgcctc gactcagaat cccgtcggcc gtgcgcgtga gaccgcagaa
     1561 tcgcgcgtct cgcggccgct ccatgcgtgg ccgtggtccg attcgggtcg cccgggtatc
     1621 acgaccgcga tccgggaacg tggcgttaac acgactccgt atcatcatcg gcaaatggca
     1681 cgaacgatcc tggtcgtcga ggacgacgcg accctgcgcg agaccctcgc cgacgccctc
     1741 gaggtcgagg ggttccgggt gatcgccgcc gccgacggcc gggacgcgct ggcccgcttc
     1801 cgggccgaca aaccggacct cgtcctcctc gacctgatgc tccctgagct gtccgggatc
     1861 gaggtctgcc ggatcatccg cgccgaatcc ggcgtgccga tcgtcatgct gacggccaag
     1921 gacagcgagc tcgacaaggt ggtcgggctc gagctcggcg ccgacgacta cgtcacgaag
     1981 ccgttcagcc tgcgcgagct gtcggcccgc atccgggcgc tgttccggcg atccgagcag
     2041 gtcgcggcgc ccgatgcggc cggcccggcg gtcgtcgacc tcgggcgagt ccaggtcgat
     2101 ctcgccgggc atcgcctcct tcgcgacggc gaggaggtcg cgatcaagcc gaaggcgttc
     2161 gagctcctcg cgttcctcct ccgccacccg ggccaggcct tcacccgcga ccagctcctc
     2221 cagcacgtct ggggctacga ctacgc
//
LOCUS       DATAIW010000127         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_65717, whole genome shotgun sequence.
ACCESSION   DATAIW010000127 DATAIW010000000
VERSION     DATAIW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65717"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VHM48_04180"
     CDS             complement(<1..142)
                     /locus_tag="VHM48_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2754633.1"
                     /translation="MAKKKFERTKPHVNIGTIGHIDHGKTSLTAAITKYLHLKGGGEY
                     RAF"
     gene            complement(178..2253)
                     /gene="fusA"
                     /locus_tag="VHM48_04185"
     CDS             complement(178..2253)
                     /gene="fusA"
                     /locus_tag="VHM48_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX2754634.1"
                     /translation="MARQVPLARTRNIGIIAHIDAGKTTVTERILFYTKKIYKIGEVH
                     EGAATMDWMPQEQERGITITAAATTAFWGDHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VVVFDGVAGVEPQSETVWRQADRYGVPRFCFINKLDRTGADFWRGVESIKDRLGANAV
                     PIQIPIGHEDRFEGIVDLVEMKAYYYRDDQGATFDIVEIPEALKAEAEKHRHKMIEAV
                     AEMDDELTHKYLEGEELTVAEIKRGLRLGTLTTRIIPVLTGSALRNKGVQKMLDAVVD
                     YLPSPLDVPPMIGLDARTGEEVIRTPSDSEPFSALAFKIAADPFVGKLAFFRVYSGTL
                     KAGSYVMNPTKGKRERIGRILQMHANHREEIDEVYAGDIAAAVGLKDTFTGDTLTDPD
                     HQIILESMTFPEPVIEVKIEPKTKADQDKLAIALQRLSEEDPTFRVHTDPESSETLIA
                     GMGELHLDVLVDRMVREFHVAANIGKPQVSYRETIRRAAEGKGSFIRQTGGKGQYGHA
                     IIKIEPQEKGAGYEFVDKIVGGTIPREYIKSVDAGIRETLATGIYAGFPVVDVKATLF
                     DGSYHEVDSSEMAFKIAGSMAVKLAFEKADPAILEPIMRNEVTMPEEFMGDVIGDLNS
                     RRGQIEGMESRGSTQVVRAFVPLSQMFGYVTDLRSMTQGRATSSMEFSHYAEVPASLA
                     SELVQKSKV"
     gene            complement(2276..2743)
                     /gene="rpsG"
                     /locus_tag="VHM48_04190"
     CDS             complement(2276..2743)
                     /gene="rpsG"
                     /locus_tag="VHM48_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2754635.1"
                     /translation="MPRRQSIPRMSKYHTEPANRTTARFTAKLMANGKRNLSDRILRQ
                     ALARAESQAHRPGLEVLEAALRNATPIIEVKPRRVGGATYQVPVEIRGDRRLSLGVRW
                     LVQAARKRNGKSMSDKLAQELVDAMNGLGAAVKRREDTHKMAEANRAFSHFKW"
     gene            complement(2743..3174)
                     /gene="rpsL"
                     /locus_tag="VHM48_04195"
     CDS             complement(2743..3174)
                     /gene="rpsL"
                     /locus_tag="VHM48_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX2754636.1"
                     /translation="MPTISQLVRHGRQTKVKKVKAPAMRKNWNGLNRKQVATPGAPQK
                     RGVCLQVRTMTPKKPNSALRKIARVRLSNQMEVTAYIPGIGHNLQEHSVVLVRGGRVK
                     DLPSVKYHIIRGTLDAAGVRDRKQGRSKYGAKVATGPGGKK"
BASE COUNT          476 a         1113 c         1200 g          679 t
ORIGIN      
        1 cgaaggcgcg gtactcaccc ccacccttca ggtggaggta cttggtgatc gccgccgtca
       61 ggctcgtctt gccatggtcg atgtggccga tcgtgccgat attgacgtgc ggcttggtgc
      121 gctcgaactt cttcttggcc atcgtgattg ctccctggct cctgcccctc ggggcgccta
      181 gaccttcgac ttctgaacga gctccgaggc caggctggcc gggacctcgg cgtagtgact
      241 gaactccatc gacgacgtcg cgcgaccctg ggtcatcgac cgcaggtccg tgacgtagcc
      301 gaacatctgg gacagtggga cgaaggcccg gacgacctgg gtcgaaccgc gcgactccat
      361 tccctcgatc tgtccgcgac ggctgttgag gtcgccgatc acatcgccca tgaactcctc
      421 gggcatcgtg acctcgttcc gcatgatcgg ttcgaggatc gccgggtcgg ccttttcgaa
      481 ggccagcttg acggccatcg atccggcgat cttgaacgcc atctccgacg agtcgacctc
      541 gtggtacgag ccgtcgaaga gggtcgcctt gacgtcgacc accgggaacc cggcgtagat
      601 gcccgtggcg agcgtctcgc ggatgccggc gtcgacggac ttgatgtatt cgcgcgggat
      661 cgtgccgccg acgatcttgt cgacgaactc gtagcccgcg cccttctcct ggggctcgat
      721 cttgatgatc gcgtggccgt actggccctt gccgccggtc tggcggatga agctgccttt
      781 gccctcggcc gcgcgccgga tcgtctcgcg gtaggagacc tgtggcttgc cgatgttggc
      841 ggccacgtga aactcgcgga ccatccggtc gacgaggacg tcgaggtgga gctcgcccat
      901 tccggcgatc agcgtctcgg acgattccgg gtcggtgtgg acccggaacg tcgggtcctc
      961 ctcggacagg cgctggagcg cgatcgcgag cttgtcctgg tcggccttgg tcttcggctc
     1021 gatcttgacc tcgatgacgg gctccgggaa cgtcatcgac tcgaggatga tctggtggtc
     1081 gggatcggtc agggtgtcac cggtgaaggt gtccttgagg ccgaccgcgg cggcgatgtc
     1141 gccggcgtag acctcatcga tctcctcgcg atggttggcg tgcatctgca ggatgcggcc
     1201 gatccgctcc ctcttgccct tggtcgggtt catgacgtac gacccggcct tgagcgtccc
     1261 ggaatagacc cggaagaagg cgagcttgcc gacgaacggg tcggccgcga tcttgaaggc
     1321 gagggcgctg aacggctcgc tgtcgctcgg ggtccggatg acctcttcgc ccgtccgcgc
     1381 gtcgaggccg atcatcggcg ggacatcgag cggcgagggg aggtagtcga cgactgcgtc
     1441 gagcatcttc tggacgccct tgttgcggag cgccgagccg gtcaggaccg ggatgatccg
     1501 ggtcgtgagg gtcccgaggc gcagtccgcg cttgatctcg gcgaccgtca gctcctcgcc
     1561 ctcgaggtac ttgtgggtca gctcgtcgtc catctcggcg acggcctcga tcatcttgtg
     1621 gcggtgtttc tcggcctcgg ccttgagggc ctcgggaatc tcgacgatgt cgaaggtcgc
     1681 cccctggtca tcgcggtagt agtaggcctt catctcgacg aggtcgacga tgccctcgaa
     1741 gcgatcctcg tggccgatcg ggatctggat cggcacggcg ttggcgccca ggcggtcctt
     1801 gatcgactcg acgccgcgcc agaagtcggc gccggtccgg tcgagcttgt tgatgaagca
     1861 gaaccgcggc acgccgtagc ggtcggcctg gcgccagacc gtctcggact gcggctcgac
     1921 accggccacg ccgtcgaaca ccaccaccgc gccgtcgagg acgcggaggc tccgctcgac
     1981 ctcgaccgtg aagtccacgt gcccgggcgt atcgataatg ttgatccggt gatcgcccca
     2041 gaacgcggtc gttgcggcag cggtgatcgt gatcccccgc tcctgctcct gcggcatcca
     2101 gtccatcgtc gcggcgcctt cgtggacctc gccgatcttg tagatcttct tcgtgtagaa
     2161 gaggatccgc tcggtcacgg tcgtcttgcc cgcgtcgata tgggcgatga ttccgatgtt
     2221 ccgcgtgcgt gcaagcggaa cctgtcgtgc cacggtgttc tctatgctcc cggatctacc
     2281 acttgaaatg gctgaaggcg cggttcgcct cggccatctt gtgcgtgtct tcacggcgct
     2341 tgacggcggc gcccagcccg ttcatcgcgt cgacgagctc ctgcgcgagc ttgtcgctca
     2401 tgctcttgcc gttgcgcttg cgagccgcct ggacgagcca gcggacgccg agcgagaggc
     2461 ggcgatcccc acggatctcg accgggacct ggtaagtcgc gccaccgacc cggcggggct
     2521 tgacctcgat gatcggggtc gcgttgcgga gggcggcctc gaggacctcg agacccgggc
     2581 ggtgggcctg ggactcggcc cgggcgagcg cctggcggag gatccggtcg gacaggttgc
     2641 gcttgccgtt ggccatcagc ttggcggtga agcgggcggt cgtccggttg gcgggctccg
     2701 tgtggtactt ggacatccgc gggatcgact gacggcgggg cattacttct tgcctccggg
     2761 gccggtcgcg accttggcgc cgtacttgct gcggccctgc ttgcggtcac ggacgccggc
     2821 cgcgtcgagg gtgccgcgga tgatgtggta cttgaccgac gggaggtcct tcacgcggcc
     2881 gccccggacg aggacgacgg agtgctcctg caggttgtgg ccgatgcccg ggatgtaggc
     2941 cgtgacctcc atctggttgg acagccggac gcgggcgatc ttgcgcagcg ccgagttcgg
     3001 cttcttcggc gtcatcgtcc ggacctggag gcagacgccg cgcttctgcg gggcgccggg
     3061 cgtggcgacc tgcttgcggt tcagcccatt ccagttcttg cgcatggccg gcgccttgac
     3121 cttcttgacc ttggtctggc gaccgtggcg cacgagctgg ctgatcgtcg gcacgaagac
     3181 gctgctcctc tcatcggtaa cggttctgtg ggcggaggtc gccggcgggt ggccggttcg
     3241 cgagcggtca ggctcgcgga ggaccttcct ggggtggact gtcgtctggg gtggtcgacg
     3301 cgcacgtcgg ccgcggaccc ctcccgaggc cacgaaggcg gagtgtatca gcggcccgaa
     3361 aacggtgtca aacctcgggc cgcgtacctg aagaccgatc cgatccttgg cccgacgtgc
     3421 aggcttcggt gatgaccgaa ccgacgtttg cacccagcct cttcgcca
//
LOCUS       DATAIW010000128         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211148, whole genome shotgun sequence.
ACCESSION   DATAIW010000128 DATAIW010000000
VERSION     DATAIW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211148"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1641
                     /locus_tag="VHM48_04200"
     CDS             160..1641
                     /locus_tag="VHM48_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754637.1"
                     /translation="MTIIAEVPTPTKIVFGTDGWRAKVADDYTFENVRRCADGVARYV
                     VAMGAQAKGVVVAFDRRFASEHFAAATAEVLLAHDIPVFLSTNAVPTQMSSFEVVERG
                     AAAGIVITASHNPWTDNGFKVKAPSGAAAGADILSVVEAAIAENGGTAIERRPLADAE
                     AAGLVERFDPFDGYERFVSRTIDLDALKAADLAILVEPLYGVGAGWIPRLLRDGRVRI
                     TEIHSERNPYFGGINPEPIRPNVDEALGLMAGGAFDLGLMLDGDADRAGAADERGTFI
                     HQLQVTGLLMYHLAEHRGLREPVVISVNNTSMAERLGAHFGITVHETPVGFKFIGPEM
                     IATGAMLGAEESGGFGFGMHLPERDGIYADLMLLELFLAEKAAGRWPVSKALAHFHEI
                     AGPSFYRRIDVHVDRAKYTETKRHLLVDLRDQAPTALDGQPIARTVPLDTNDGFKFYL
                     ADGTWLLIRASGTEPLVRVYTEATSADLREAMLVAGERLVRGS"
     gene            1638..2027
                     /locus_tag="VHM48_04205"
     CDS             1638..2027
                     /locus_tag="VHM48_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2754638.1"
                     /translation="MSDATAAGAFEPRRVDKPWGSELIWALTDRYCGKVITIETGKRL
                     SLQYHEQKVESILVLSGRLLLHLEDDAGVMTKRELRAGDTSHVPVGRRHRFEALERVE
                     LVEVSTPELGDVVRVEDDFGREGTSNP"
BASE COUNT          319 a          790 c          773 g          308 t
ORIGIN      
        1 cgagcaggcc ccggacctcg tcgatgaggt ggcgggcgcc ggcggctcgg gcggtacgat
       61 cgtcgccgac ggcgtgtggg cccacctcgc cggcgcccgc cacctcatcg acgaggtccg
      121 gggcctgctc gagcgggtcg atcaccagga ggtccgcgcg tgacgatcat cgccgaggtg
      181 ccgacgccga cgaagatcgt gttcgggacc gacggctggc gggccaaggt tgccgacgac
      241 tacacgttcg agaacgtccg ccgctgcgcc gatggcgtcg cccgctacgt cgtcgcgatg
      301 ggcgcccagg cgaagggggt cgtcgtcgcc ttcgaccggc ggttcgcctc ggagcatttc
      361 gccgccgcga ccgccgaggt cctcctcgcc cacgacatcc cggtgttcct ctcgacgaac
      421 gcggtgccga cccagatgag ctcgttcgag gtcgtcgagc gcggagccgc ggccggcatc
      481 gtgatcaccg cgtcgcacaa cccgtggacc gacaacgggt tcaaggtcaa ggcgccgagc
      541 ggggcggcgg ccggcgccga catcctctcg gtcgtcgagg cggccatcgc cgagaacggc
      601 ggcaccgcga tcgagcgccg gcccctggcc gacgcggagg cggccggact cgtcgagcga
      661 ttcgacccgt tcgacggcta cgagcgtttc gtcagccgga cgatcgacct cgatgcgctc
      721 aaggcggccg acctggcgat cctcgtcgag ccgctgtacg gcgtcggcgc cggctggatc
      781 ccgcgcctgc tgcgcgacgg ccgggtccgg atcaccgaga tccacagcga gcgcaacccg
      841 tacttcggcg ggatcaatcc cgagccgatc aggccgaacg tcgacgaagc cctcggcctg
      901 atggccggcg gggccttcga cctcggcttg atgctcgacg gcgacgccga ccgggcgggt
      961 gccgccgacg agcgggggac gttcatccac cagctccagg tcacggggct cctgatgtac
     1021 cacctcgccg agcaccgcgg gctgcgcgag ccggtcgtga tcagcgtcaa caacacctcg
     1081 atggccgagc ggctcggcgc gcattttggg atcaccgtcc acgagacgcc ggtcgggttc
     1141 aagttcatcg gcccggagat gatcgcgacc ggggcgatgc tcggtgccga ggagtcgggc
     1201 ggcttcgggt tcgggatgca cctcccggag cgcgacggca tctacgccga cctgatgctg
     1261 ctcgagctgt tcctcgccga gaaggccgcc ggccgctggc cggtgtccaa ggcacttgcc
     1321 cacttccacg agatcgcagg cccgtcgttc taccgccgga tcgacgtcca cgtcgaccgg
     1381 gcgaagtaca ccgagacgaa gcgccacctg ctcgtcgacc tccgcgacca ggcgccgacc
     1441 gcgctcgacg gccagccgat cgcgcggacc gtcccgctcg acacgaacga cgggttcaag
     1501 ttctacctgg ccgacggcac gtggctcctg atccgggcga gcgggaccga gccgctcgtc
     1561 cgcgtctaca cggaggcgac ctcggccgac ctgcgcgagg cgatgctcgt cgccggcgag
     1621 cggctggtcc gcggatcgtg agcgacgcca cggctgcagg cgccttcgag ccgcgtcgcg
     1681 tcgacaagcc ctggggcagc gagctgatct gggccctgac cgaccgctac tgcggcaagg
     1741 tgatcacgat cgagaccggc aagcggctgt cgctccagta ccacgagcag aaggtcgaat
     1801 cgatcctggt cctgagcggc cggctgctgc tccatctcga ggacgacgcc ggcgtcatga
     1861 cgaagcgcga gctccgggcc ggcgacacgt cgcacgtccc ggtcggcagg cgtcaccggt
     1921 tcgaggcgct cgagcgcgtc gagctcgtcg aggtctccac cccggagctc ggcgacgtcg
     1981 tccgggtcga ggacgacttc ggccgcgagg ggacctccaa cccgtagtgc gccatccggg
     2041 ggccatgacc gtctcgccat tccgtcggag ccgcctgacg tccctcatcc tgatggcggc
     2101 gatcgtcgcg gccgcctgcg attcgactgc gaccccgggc tccgccacgc cggtcgggtc
     2161 cggtgccgga ccgacggccg aggcgtcggg
//
LOCUS       DATAIW010000129         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_5735, whole genome shotgun sequence.
ACCESSION   DATAIW010000129 DATAIW010000000
VERSION     DATAIW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5735"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VHM48_04210"
     CDS             complement(<1..1045)
                     /locus_tag="VHM48_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754639.1"
                     /translation="MNDLRRQPFVWTWAVLPILIVAVAVRIALWPTPGLPGDLDQFVL
                     WVNGIATHGLANAYDQNLSFPPVMAYVWGVLAAIEPAFRTATTAADPAIRAIMKAPAS
                     LADLALGLIVVWHLRATPRWALLGGAAIVLHPAVIDVSAWWGQYESIYVLAGVVAYVL
                     AVRGHSLWAAAALGVALMTKPQALPFLVPFGAWFLARDGWRGAARAALVGAVVIAILW
                     LPFLAAGGVQAYARNLTTYQGDIFAILSLRAWNAWWLVQELFAGGQFVSDQGAILGPI
                     TLRHVGYGLALLGEVAVFALVYRARSARALAYGLAAAVLVAFCLLTTMHERYAFGALA
                     FLVLAFPDRRAAWL"
     gene            1137..1349
                     /locus_tag="VHM48_04215"
     CDS             1137..1349
                     /locus_tag="VHM48_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754640.1"
                     /translation="MEKQNRAERRKGKFGRGRATEHGGWPTVRPNPVFTGDTAADDAP
                     KSTPDTDPAAGQAPDNQDPDASDAKV"
     gene            complement(1476..>2591)
                     /locus_tag="VHM48_04220"
     CDS             complement(1476..>2591)
                     /locus_tag="VHM48_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2754641.1"
                     /translation="YTRPAMTHVFVAPHPDDVALSCGGLIAGLRELGQNVTILTVFSG
                     AGPTAGGGGGDTLSTYQREALGFGSKTMWPVTEAFNRSHISADFPVYGDPELLPAWAA
                     TDDRLEATQADADAAAKRFWQRSSWYRRASIRGESLAGQPIMDTLSTQGAVATDELVD
                     AAAAGEVTAQRRLEDEAFAAFAEASIVFLDLPDAVFRGYASDEELLNAPRDDDAAPFD
                     ILRREIARLEPQKLYLPLGIGNHVDHQLCREVGVRLLGESRRWVMPGPEYAGNVTFYE
                     DFPYAWWNDFKHLEDLPRTAFADLPAGISLMPEYADIGDQLERKITGINLYASQMDRL
                     FSGTQEMARQVRAYARRVALLDGSGGVAERYWSTVAS"
BASE COUNT          425 a          923 c          935 g          308 t
ORIGIN      
        1 ccagccacgc ggcccgccga tccgggaagg cgaggacgag gaacgcgagc gccccgaacg
       61 cgtagcgttc gtgcatcgtc gtcagcaggc agaacgcgac caacacggct gcggcgagcc
      121 cgtaggcgag ggcccgcgcc gatcgggcgc ggtacacgag ggcgaacacc gcgacctcgc
      181 cgagcagggc gagcccgtac ccgacgtgac gcagcgtgat gggtccgagg atcgcgccct
      241 ggtccgacac gaactgcccg ccggcgaaca gctcctggac gagccaccag gcgttccacg
      301 cccgcagcga caggatcgcg aagatgtcgc cctggtaggt cgtgaggttg cgggcgtagg
      361 cctggacgcc gccggcggcg aggaacggca gccacaggat cgcgatcacg acggcgccga
      421 ccagggccgc ccgcgccgcc ccgcgccagc cgtcccgggc caggaaccac gccccgaatg
      481 gcacgaggaa ggggagggcc tgcggcttcg tcatgagcgc gaccccgagc gcggccgccg
      541 cccacagcga gtgcccgcgg accgcgagca cgtacgcgac cacgccagcg agcacgtaga
      601 tcgactcgta ctggccccac cacgcgctga cgtcgatcac cgccgggtgg aggacgatcg
      661 ccgccccgcc gaggagggcc cagcgcggcg tcgctcggag gtgccagacg acgatcagcc
      721 cgagggcgag gtcggccagg ctcgcgggcg ccttcatgat cgcccgaatc gccggatcgg
      781 ccgcggtcgt cgccgtccga aacgcgggct cgatcgcggc caggacgccc cagacgtagg
      841 ccatgaccgg cgggaacgac aggttctggt cgtaggcgtt ggcgaggccg tgcgtcgcga
      901 tcccgttgac ccagaggacg aactggtcga gatcgccggg caggcccggc gtcggccaca
      961 gcgcgatccg gacggccacc gcgacgatca ggatgggaag gaccgcccac gtccagacga
     1021 agggttggcg tcggaggtcg ttcacggccg gatcatccta tcgtcggccg accccgccaa
     1081 cgggcccgat cgtcctgccc gctccacgtg cggcgtggcc gtgcgaccat gcgctcatgg
     1141 agaagcagaa ccgcgccgag cggcgcaagg gcaagttcgg ccgcggccgc gcgaccgagc
     1201 acggcggctg gccaaccgtc cggccaaacc cggtgttcac cggcgacacc gccgcggacg
     1261 acgcgccgaa gtcgacgccc gacacggatc cagctgccgg tcaggccccc gacaaccagg
     1321 atcccgacgc gagcgacgcc aaggtctgac gggcaggacc aaccggcgag cggcacccgc
     1381 cgcgcgcgcc gtgcggcgca aggtattcgg ctattcgaaa cggcagcgga gtatgtattc
     1441 gcccgcggcc cgccgcccgc ggcaatcgcg gcctcttacg aggcgaccgt cgaccagtac
     1501 cgctcggcga cgccgcccga cccgtcgagc agcgcgaccc gacgcgcata cgcccggacc
     1561 tggcgggcca tctcctgggt cccgctgaac agccggtcca tctggctcgc gtacaggttg
     1621 atgccggtga tcttgcgctc gagctgatcg ccgatgtcgg cgtactcggg catcagcgag
     1681 atccccgccg gcagatcggc gaacgcggtc cggggcaggt cctcgaggtg cttgaaatcg
     1741 ttccaccaag cgtaggggaa gtcctcgtag aacgtcacgt tccccgcgta ttcgggaccg
     1801 ggcatcaccc agcggcggct ctcgccgagg agccgcacgc cgacttcgcg gcagagctgg
     1861 tggtcgacgt ggttgccgat cccgagcggc aggtacagct tctggggctc gaggcgggcg
     1921 atctcgcggc ggaggatgtc gaacggcgcc gcatcgtcgt cgcgcggcgc gttcaggagc
     1981 tcctcgtcgc tggcgtagcc ccggaacacc gcgtcgggca ggtcgagaaa gacgatcgac
     2041 gcctcggcga acgccgcgaa ggcctcgtcc tcgagacggc gctgggccgt gacctcgccg
     2101 gcagcggcgg catcgacgag ctcgtcggtc gcgacggcgc cctgcgtcga gagcgtgtcc
     2161 atgatcggct ggccggccag cgactcgccc cggatgctcg cccggcgata ccacgacgac
     2221 cgctgccaga agcgcttggc ggcggcatcc gcgtcggcct gggttgcttc gaggcggtcg
     2281 tccgtcgccg cccaggccgg cagcagctcg gggtcgccgt agaccgggaa gtcggccgag
     2341 atgtgcgatc gattgaaggc ctcggtcacc ggccacatcg tcttggaccc gaagccgagc
     2401 gcctcgcgct ggtacgtcga gagcgtgtcg ccgcctccgc cgcccgcggt cggaccggcc
     2461 ccggagaaga ccgtcaggat cgtcacgttc tgaccgagct cccggaggcc ggcgatcagc
     2521 ccaccgcagg agagggcgac gtcgtcggga tgcggggcga cgaagacatg ggtcatggcc
     2581 gggcgagtat a
//
LOCUS       DATAIW010000130         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_5793, whole genome shotgun sequence.
ACCESSION   DATAIW010000130 DATAIW010000000
VERSION     DATAIW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5793"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VHM48_04225"
     CDS             complement(<1..992)
                     /locus_tag="VHM48_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2754642.1"
                     /translation="MTAGAPSLPSILGPIRPRPRGRESRLLAIVAITILVGSVSLELA
                     KRITVPGESQGLSPANPLHLAIYLGLIGIAHLVLILSGRRMDQIILPATAMLGGISLL
                     LMERLPQTQVQQQVGPWKLGLGDIQLAWLILSFSLATILAVTVRSDRWLRLYKYTWAA
                     AGVGLLVATYFLGDDINGQRLSLSLGPISGQPAELLKVILVVFLAGYLSEYRPLLVEE
                     STKVGPISLPPLPFLAPMVAMWAIALGLVVVQRDLGAALLFFTVFLCLLYAATGRVGF
                     VVVGVLLFLAGSFVMYHLVPTVQTRVDIWLDPFKSPSGDGYQIVQSLYAFARGGL"
     gene            complement(989..1441)
                     /locus_tag="VHM48_04230"
     CDS             complement(989..1441)
                     /locus_tag="VHM48_04230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2754643.1"
                     /translation="MDPFLLTVWAVRLTFLGLLYFFLFRIARALVGDLRAAAREPGAE
                     LGRLVVIASPAGEPEEGTSLALDAIATIGRDVNNAIVVEDQFVSAEHAILTFRGRAWY
                     LEDLGSTNGTFVNGALVEGVAPLGFGDIIQIGQVRLRLERPRADRSRR"
     gene            complement(1441..2367)
                     /locus_tag="VHM48_04235"
     CDS             complement(1441..2367)
                     /locus_tag="VHM48_04235"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX2754644.1"
                     /translation="MGPLAAVERFLERLFERQSARLFRTAIRPVQVQRRLERVMEGDR
                     TRDGARTLVPHRFIVRLHPDDLAALRTAAPQLASTLADGALAFARSHGYTLLDRPVVA
                     LRSDPAVDPGDIAVVALDPHAAPEADPHDGREAAGAGEPPPAGDAAPAGDAARPGAAA
                     ATDIGPVAAAHQTAVFVLPGIDGPRATIREIRPDQSSRSIAFDGRPLTIGRAPDNGIV
                     LRDGRASRHHARIDGRRGSLVLSDLGSTNGSFVNDRRVEAIALGEGDRIRIGTTTLLV
                     EAIAAPAPRSAGGAPAGGGPAADDPGRGGANS"
     gene            2605..>3697
                     /locus_tag="VHM48_04240"
     CDS             2605..>3697
                     /locus_tag="VHM48_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754645.1"
                     /translation="MPEILTESFCERCGTRYTFESAAPRKTRRLGQFKTLSKGMKNWV
                     MSDDTSLDEAMAAARNDDDRELTSHQLDAFHATFNFCMTCRQYTCGNCWNAAEGHCLT
                     CAPHLGHEILPAPFPDQAAFEPVRIEAEAWPEVDLSVPGVAAANGNGHDHGLQPDTVD
                     PWASVGAGDVDEILEIDPAARLAFLSGETLSPVVEPEPTPAAAAAPIDRPETTRTSTG
                     EVLWTPTFLGTANVRAAEPVASRADADVEADAPAPAIETAPVVADAPVPHAPTAAAAS
                     IVEPLPPTIEGRADAGAARTSDLLRRFRPGQNIDAELAAYEAELDLAAPATTHPDPAG
                     ELTAAAESPIEPGVVAEPVVAEPVVAEPWV"
BASE COUNT          582 a         1281 c         1373 g          461 t
ORIGIN      
        1 agcccgccgc gggcgaacgc gtagagcgac tggacgatct ggtagccgtc cccggacggg
       61 ctcttgaagg gatcgagcca gatgtcgacg cgggtctgga ccgtggggac caggtggtac
      121 atgacgaacg agccggcgag gaacaggagg acgccgacga ccacgaagcc gacccggccg
      181 gtcgccgcat agagcaggca caggaacacg gtgaagaaca ggagggcggc gccgaggtcg
      241 cgttggacga cgacgaggcc cagggcgatc gcccacatcg cgaccatcgg ggcgaggaac
      301 ggcagcggcg gcagcgagat gggaccgacc ttcgtcgatt cctcgacgag gagcgggcga
      361 tactccgaga ggtagccggc gaggaagacg acgaggatca ccttcagcag ctcggccggt
      421 tgaccgctga tcggcccgag actgaggctg agacgctggc cgttgatgtc gtcgcccagg
      481 aaatacgtgg cgacgaggag accgaccccg gccgccgccc aggtgtactt gtagagccgg
      541 agccagcgat cgctgcggac ggtgaccgcg aggatcgtcg cgaggctgaa cgacaggatg
      601 agccaggcga gctggatgtc gccgaggccc agcttccacg gcccaacctg ctgttgcacc
      661 tgggtctgcg gcaaccgttc catcagcagg aggctgatcc cgccgagcat cgcggtcgcc
      721 ggcaggatga tctggtccat ccgccgcccg gacaggatca ggacgagatg ggcgatcccg
      781 atcagcccga gatagatcgc caggtggagc gggttggccg ggctcaggcc ctgcgattca
      841 ccggggaccg tgatgcgctt ggccagctca agcgagacgc tcccgacgag gatcgtgatc
      901 gcgacgatcg cgagcaggcg cgactcccga ccgcgcggcc gcggccggat cgggccgagg
      961 atcgacggca ggctcggcgc gccggccgtc atcggcgcga tcgatcggcg cgcgggcgct
     1021 ccaggcgaag acggacctgc ccgatctgga tgatgtcgcc gaagccgagt ggtgcgacac
     1081 cctcgaccag ggcgccgttc acgaacgtcc cgttggtgct tccgaggtcc tcgaggtacc
     1141 aggcccggcc gcggaacgtg aggatggcgt gctcggccga gacgaactgg tcctcgacga
     1201 cgatggcgtt gttgacgtcg cggccgatcg tggcgatcgc gtcgagggcc agcgaggtgc
     1261 cctcctccgg ttccccggcc ggtgatgcga tgacgacgag gcggccgagc tcggcgccgg
     1321 gctcacgggc ggccgcccgg agatcgccga cgagcgcccg ggcgatccgg aacaggaaga
     1381 aatacaggag cccgaggaac gtcaggcgga cggcccagac ggtcagcagg aacgggtcca
     1441 tcaggagttc gcgccgccgc gaccgggatc gtcggcggcg ggaccgccgc cggccggagc
     1501 accgccggcg gatcgggggg ccggggcggc gatcgcctcg accagcagcg tcgtcgtccc
     1561 gatccggatc cgatcgccct cgccgagggc gatcgcctcg acccgccggt cgttgacgaa
     1621 cgagccgttg gtgctgccca ggtcggacag gacgagcgac ccacgccggc cgtcaatccg
     1681 ggcgtgatga cgcgatgccc ggccgtcgcg gaggacgatc ccgttgtcgg gcgcccgccc
     1741 gatcgtcagc ggccggccgt cgaaggcgat cgaacggctc gactggtccg gccggatctc
     1801 gcggatcgtg gcccgcggac cgtcaatacc cggcaggacg aagaccgccg tctggtgggc
     1861 agcggcgaca ggaccgatgt cggtggcggc ggccgctccc ggccgagccg cgtcgccggc
     1921 tggagccgcg tcgccggctg gaggcggctc gccggcgccg gccgcctcac gaccatcgtg
     1981 cgggtcggcc tcgggcgcgg catgcgggtc gagcgcgacg accgcgatgt cgccgggatc
     2041 gacggctgga tcggaccgga gggccacgac gggtcggtcg agaagggtgt agccatggct
     2101 ccgggcgaac gcgagggccc cgtccgcgag cgtcgaggcg agctgcggcg cggccgtgcg
     2161 cagggcggcc aggtcgtcgg gatgaaggcg aacgatgaac cggtgcggca cgagcgtccg
     2221 ggccccgtcg cgcgtgcggt ccccttccat gactcgctcg agccggcgct ggacctggac
     2281 gggacggatc gccgtccgga acagccgcgc cgactggcgc tcgaagagcc gttcgaggaa
     2341 gcgctcgacg gcggcaaggg gtcccatgtc gggggcggag tctagcagac ggtcctgaga
     2401 ggtccgcgcg ctcagcgtcg atccggggtg cgccgcggct gcggtgcgcc ggcgccgccg
     2461 cctatcggtc ccggtcgggg cggaccatcg tcccggttcg gcccgtacga gcgatggagg
     2521 ctgccaaacg gggctgcgtt atactctcgc ctcgttcggt ccgccgttcg gctccgacgt
     2581 gatcctcgcg tacccggggg catgatgccc gaaatcctca ccgagtcctt ctgcgagcgc
     2641 tgcggcacgc gttacacctt cgaatcggcc gcgccccgca agactcgtcg cctcggccag
     2701 ttcaagaccc tgtcgaaggg catgaagaac tgggtcatga gtgacgacac gtcactcgat
     2761 gaggccatgg ccgcggctcg caacgacgac gaccgcgagc tgacgagcca ccagctcgac
     2821 gcattccacg cgacgttcaa cttctgcatg acctgccgcc agtacacgtg cggcaactgc
     2881 tggaacgccg ccgaggggca ttgcctcacc tgcgcccccc atctgggcca cgagatcctg
     2941 cccgctccct tcccggacca ggccgcgttc gagcccgttc ggatcgaggc ggaggcgtgg
     3001 cccgaggtcg acctgtccgt tcccggtgtg gccgctgcca acggcaacgg ccatgaccat
     3061 ggcctccaac ccgacaccgt cgacccgtgg gcgagcgtcg gggcgggcga tgtcgacgag
     3121 atcctggaga tcgatccggc agcccgactg gcgttcctgt ccggcgagac gctgtcgccc
     3181 gtcgtcgaac ccgagccgac ccccgcggcc gccgccgcac cgatcgaccg gccggaaacg
     3241 accaggacgt cgacgggcga agtgctgtgg acgccgacct tcctcgggac ggccaatgta
     3301 cgcgccgccg agccggtcgc cagccgcgcg gacgccgacg tcgaggcgga cgcaccggcg
     3361 ccggccatcg agacggcgcc ggtcgtcgcc gatgcccccg tgccgcacgc gccgaccgca
     3421 gccgcggcgt cgatcgtcga gcccttgccg ccgaccatcg aggggcgcgc ggacgccggc
     3481 gcggcccgga cgtccgacct cctgcggcgc ttccggcccg ggcagaacat cgacgccgag
     3541 ctcgcggcct acgaggccga gctcgacctg gccgccccgg ccacgaccca tccggacccg
     3601 gccggcgagc tgacggctgc ggccgaatcg ccgatcgaac cgggcgtcgt cgcggagccg
     3661 gtcgtcgcgg agcccgtcgt cgcggagccg tgggtgg
//
LOCUS       DATAIW010000131         7037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_270886, whole genome shotgun sequence.
ACCESSION   DATAIW010000131 DATAIW010000000
VERSION     DATAIW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270886"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VHM48_04245"
     CDS             <1..1502
                     /locus_tag="VHM48_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754646.1"
                     /translation="DGSEMTTECDTVVVALGLAPRDLLARMTRDEGVTAVGPAADDHP
                     LPPAPTAGVVCLCMGTTVADLDVAFAKGYTELELLKRASWAGLGPCQGGACLPHVRAF
                     LAAKTGVVPEPFTARPAVRQITLAEAAADTTIDAFRRTPLHDEHLAAGARMDRFGGWW
                     RPWHYGDVVGEYWAVREGVSIGDVSTLGKLVVSGPDVVEALERIYPCNVTDIKVGRSR
                     YALLLNERGHVMDDGMVLRDAETRFTLTFTSGGAANAEMWIRDWVDTWGLRVHVMDRT
                     MSLAAINVTGPFAKALLGRLGLTDAPRFLGHVRAEVDGVPCHVMRLSFTGETAFELHH
                     PIDQSVGLWHALLDHGEDLGIRPHGLQALFGLRLEKGHVIVGMDTELDTTPRRLGMDW
                     AVRMEKPAFIGRASLERTAKLDDERRWFGFAMDGEAPPEGSPIRAPGGSGEIIGNVTG
                     SWKSPLLGKALILGWQRRRPFLDRVEIDGREAVVRRTPFYDPEGLRARA"
     gene            1489..1896
                     /locus_tag="VHM48_04250"
     CDS             1489..1896
                     /locus_tag="VHM48_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754647.1"
                     /translation="MPALEPVSGIRVVAAPAALDGARWQGDDVDVIRIAPDEAFGIRA
                     VGVDVDDPDAIVEPETGFAVALLDADDQYGVVDHLEWALPEEPRVLAQGKVAGVPARI
                     RTGDPATMPLGEPALLVTNNAYANELERRLGWR"
     gene            1887..3110
                     /locus_tag="VHM48_04255"
     CDS             1887..3110
                     /locus_tag="VHM48_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754648.1"
                     /translation="MALTAAAEAVESLQPIRWHEPRDSYDVVIVGGGGHGLATAYYLA
                     TRHGITNVAVLEADYIASGNTGRNTTIIRANYAIPEAIRFYDHSMKLYQDLEAETGAA
                     IFHREKGHVWLAHSEMGMRTERGRCLMNQAMGVRTEMLTPAEVKELVPQIDLTGGGRY
                     PVFGASHHVEAATARHDRVAWAFASGATGRGVHILQHRPVTGLLRDGDRVVGVRTAAG
                     DIRAGIVLSAVGGRVTEMAAHAGVRLPIRTHPLHAFVTNDFAQGLDKIVASTELACYV
                     SQTERGQMLIGAEFDSQPSFSRISSFDALRSYAYKITMLLPFLRPMRILRTWAGLCDI
                     SADWSPIMGETGVDGFLITTGWGTWGFKAIPAGGEALAERIATGKPPALIAPFGLDRF
                     RRDHVLADQASAGTR"
     gene            3110..3352
                     /locus_tag="VHM48_04260"
     CDS             3110..3352
                     /locus_tag="VHM48_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX2754649.1"
                     /translation="MHTLDCPRCGRRPLDEFTFGGERRPSPSSIDDPDARDFDEVWIF
                     DNPDGIATERWFHAAGCRRWLTVRRDTSIDRVLEIH"
     gene            3352..4545
                     /locus_tag="VHM48_04265"
     CDS             3352..4545
                     /locus_tag="VHM48_04265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2754650.1"
                     /translation="MFEGYAARRLDIAVRDRARFGAGVVTELPSIVASLGAQAAFVVT
                     DSGVVGSGVAGRVVDLLADAGLRVGLFDGVEPNPGASSVARGSAALRRFQELAGGTSA
                     AVVGLGGGSAMDSAKAIALHATNDRDVMSLGYHDETLATGIPVIAIPTTAGTGAETNT
                     YGVITDEVAGRKGYIGHESVLPRVAILDPELTIGLPSGPTAATGVDAMTHSLESLLSR
                     NPNPFAEAIALGVIRTVAEWLPRAVDDGTDLEARSRMLLAAHYAGVGQQSGTGVGAVH
                     AIGHAIGTRGRLAHGTALATVMPEVFATYLEVRERELALVAAALGIADPRDEPGEVAR
                     AAIDGIDALLRSVGQRRTLAEQGLGPETHETIAQDSIEDAAINNSPRLPSKREILAIL
                     AAVAG"
     gene            complement(4709..5140)
                     /locus_tag="VHM48_04270"
     CDS             complement(4709..5140)
                     /locus_tag="VHM48_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754651.1"
                     /translation="MIGSTIGRLLAVFDTPAEADAAVAGLTSLPAIDAARIERFDRPE
                     DAPVFDASGSRRGWRGRLVQILEFSWADQAVDFAWYEAAVREGHVVLSVAVRGQTAMG
                     AAADVLTANGAHFINHYGWLETQELALWRGPEPDLPEFLRR"
     gene            complement(5175..6125)
                     /locus_tag="VHM48_04275"
     CDS             complement(5175..6125)
                     /locus_tag="VHM48_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2754652.1"
                     /translation="MTDLKLGILLWNQATDWPSYLAAVKRMDALGYDHLWSWDHLYAI
                     FGDPYQPIFEGWSSLVAAAASTERIRVGLLVGANTFRNPGVVAKTAATLDHVSGGRAI
                     LGLGGAWMDLEHEAHGIEFGSSIGQRLDWMDESAAAMRALLDGKEITSVPGGHYAFDH
                     ARHQPPPVQAHLPIMIGGSGEKKTLRTVARYADLWNAMGPVEVMRHKIEVLRRHCEDV
                     GRDPAEIEFTLGIKATIRDSEAEADRVWKAAMEHNRTPLAEVADDDTFWNGTAEQLAE
                     KLAPYVELGFHTVISEQPAPYDMETFERLIGEVKPLVDAG"
     gene            complement(6486..>7037)
                     /locus_tag="VHM48_04280"
     CDS             complement(6486..>7037)
                     /locus_tag="VHM48_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754653.1"
                     /translation="RLTRLLHVVGRMTDGKDPIMSNATTEATTATAVELDSATQRQLG
                     VDLYNSTWTLIEKADRTAAETDEMIHRAHTSRWHWARVGEPVNLARGEWLCSRVYATL
                     GRGEPALWHARRCVEINESIPADTRESWDLPAAYEAMARASFAAGDPASGALWKAKAT
                     AALEGIVDADDREPIAQDIATLP"
BASE COUNT          978 a         2479 c         2531 g         1049 t
ORIGIN      
        1 ccgacgggtc cgagatgacg accgagtgcg acaccgtcgt cgtcgccctc ggcctcgccc
       61 cgcgggacct cctcgcccgg atgacccgcg atgagggcgt gaccgcggtg ggcccggcag
      121 ccgacgacca tccgctgccg cccgcgccga ccgccggcgt cgtctgcctg tgcatgggca
      181 cgaccgtcgc cgacctggac gtcgccttcg ccaagggcta caccgagctc gagctgctga
      241 agcgcgcgtc gtgggccggc ctcgggccgt gccagggcgg cgcatgcctg ccccatgtcc
      301 gggcgttcct ggccgcgaag accggcgtcg tccccgagcc gttcaccgcg cgcccggccg
      361 tccgccagat caccctcgcc gaagcggccg cggacacgac gatcgacgcg ttccggcgaa
      421 cgccccttca cgatgagcac ctcgcggccg gggcccggat ggaccggttc ggcggatggt
      481 ggcgaccgtg gcattacggc gacgtcgtcg gtgagtactg ggccgtccgc gagggcgtct
      541 cgatcggcga cgtctcgacg ctcggcaagc tggtcgtcag cggtccggac gtcgtcgagg
      601 cgctcgagcg gatctacccg tgcaatgtca cggacatcaa ggtcggccgg tcgcgctacg
      661 cgctgctcct caacgagcgc ggccacgtga tggacgacgg gatggtcctg cgcgacgcgg
      721 agacgcggtt cacgctgacc ttcacatccg gcggcgcggc caacgccgag atgtggatcc
      781 gcgactgggt cgacacctgg ggcctccggg tccacgtgat ggaccgcacg atgtccctcg
      841 ccgcgatcaa cgtgacgggg ccgttcgcga aagcattgct gggccggctc gggctgacgg
      901 atgcgccgcg ctttctcggc cacgtccggg ccgaggtcga cggcgttccg tgccacgtca
      961 tgcggctgtc gttcacgggc gagacagcgt tcgagctcca ccacccgatc gatcagtccg
     1021 tgggcttgtg gcacgcgctc ctcgatcacg gcgaggacct cggaatccgg ccgcacggcc
     1081 tccaggcgct gttcgggctg cggctcgaga agggccacgt gatcgtcggg atggacacgg
     1141 agctcgacac gacgccgcgc cggctcggga tggactgggc ggtccggatg gagaagccgg
     1201 cgttcatcgg ccgagcgtcg ctggagcgga ccgcgaagct cgacgacgag cggcgctggt
     1261 tcgggttcgc gatggacggg gaggcgccgc cggagggctc gccgatccgg gcgcccggcg
     1321 gcagcggcga gatcatcggc aacgtgacgg ggtcgtggaa atcgccgctc ctcggcaagg
     1381 ccctgatcct cggctggcaa cgccgccggc cgttcctcga ccgcgtcgag atcgacggtc
     1441 gagaggccgt cgtgcggcgg acccccttct acgacccgga ggggctccgt gcccgcgctt
     1501 gagccggtgt cgggcatccg ggtcgtggcc gcaccggcgg cgctcgacgg cgcccgctgg
     1561 caaggcgacg atgtcgacgt gatccggatc gcgccggacg aggcgttcgg gatccgcgcc
     1621 gtcggtgtgg acgtcgacga tccggacgcg atcgtcgagc ccgagaccgg cttcgccgtg
     1681 gcgctgctcg acgccgatga ccaatacggg gtcgtcgatc acctcgagtg ggcgctcccc
     1741 gaggagcccc gtgtcctcgc ccagggcaag gtcgccggcg tgcccgcgcg gattcggacc
     1801 ggagacccgg cgacgatgcc gcttggcgag cccgcactcc tcgtcacgaa caacgcctac
     1861 gccaacgagc tcgagaggcg gctcggatgg cgctgaccgc cgcggccgag gccgtcgaga
     1921 gcctccagcc gatccgctgg cacgagccac gcgattccta cgacgtcgtc atcgtggggg
     1981 gcggcggcca cggtctcgcg accgcgtatt acctcgctac gcgccacggg atcaccaacg
     2041 tcgccgtcct cgaggccgac tacatcgcgt cgggcaacac cggccggaac acgacgatca
     2101 tccgggcgaa ctacgcgatc cccgaggcga tccgcttcta cgaccactcg atgaagctct
     2161 accaggacct cgaggccgag accggcgccg cgatcttcca tcgcgagaag ggccacgtct
     2221 ggctcgccca ttcggagatg gggatgcgga ccgagcgcgg ccgctgcctg atgaaccagg
     2281 cgatgggcgt ccggaccgag atgctgacgc cggccgaggt caaggagctc gtcccgcaga
     2341 tcgacctgac cggcggcgga cgctacccgg tcttcggggc gtcgcaccac gtcgaggcgg
     2401 ccaccgcccg acatgatcgg gtcgcctggg cgttcgcgtc aggggcgacg gggcgtggcg
     2461 tccacatcct tcagcatcga ccagtcacgg gacttctccg cgacggcgac cgggtggtcg
     2521 gcgtccggac cgccgcaggc gacatccggg ccgggatcgt cctctccgcg gtcggcggcc
     2581 gggtgaccga gatggccgcc cacgccggcg tccgcctgcc gatccggacc cacccgcttc
     2641 acgccttcgt caccaacgat ttcgctcagg gcctcgacaa gatcgtcgcc tcgaccgagc
     2701 tcgcctgcta cgtcagccag accgagcgcg gccagatgct gatcggcgcc gagttcgaca
     2761 gccagccgtc gttctcgcgg atctcgtcgt tcgacgcgct ccggagctac gcctacaaga
     2821 tcacgatgct cctgccgttc ctgcggccga tgcggatcct ccggacgtgg gcgggcctct
     2881 gcgacatctc cgcggactgg agcccgatca tgggcgagac gggcgttgac gggttcctga
     2941 tcacgaccgg ctgggggacg tggggcttca aggcgatccc ggccggcggc gaggccctcg
     3001 ccgagcggat cgcgaccggg aagccgcccg ctctgatcgc gccgttcggg ctcgaccggt
     3061 tccggcgcga ccatgtcctg gccgaccagg cgtcggcggg cacgcgctga tgcacacgct
     3121 cgactgcccg cgctgcgggc gacggccgct cgacgagttc acgttcggcg gcgagcgacg
     3181 accgagcccg tcctcgatcg acgatccgga cgcccgcgac ttcgacgagg tctggatctt
     3241 cgacaacccc gacggcattg cgacggaacg ctggttccat gccgcgggct gtcggcgctg
     3301 gctgaccgtc cggcgcgaca cgagcatcga ccgcgtcctg gagatccatt gatgttcgag
     3361 ggctacgcgg cgcgacggct ggacatcgcg gttcgcgacc gggcgcggtt cggagccgga
     3421 gtcgtcacgg agctgccctc gatcgttgcc tcgctcggcg cccaggccgc cttcgtcgtc
     3481 accgactcgg gcgtcgtcgg gtccggggtc gccggtcggg tcgtcgatct cctcgcagac
     3541 gccgggctgc gggtcggcct gttcgacggc gttgagccga acccgggcgc ctcgtcggtc
     3601 gctcggggca gcgctgccct gcggcggttc caggagctcg ccggcgggac gtccgccgcc
     3661 gtcgttggcc tgggcggcgg gtcggcgatg gactcggcca aggcgatcgc cctccacgcc
     3721 acgaacgacc gtgatgtcat gtcacttggc taccacgatg aaacgctcgc gacagggatc
     3781 ccggtcatcg cgatcccgac gacggccggc accggcgcgg agacgaacac gtacggcgtc
     3841 atcaccgacg aggtcgccgg acggaagggt tacatcggcc acgagagcgt cctgccgcgg
     3901 gtcgcgatcc tcgacccgga gctgacgatc ggcctgccgt cgggtccgac cgcggcgacc
     3961 ggggtcgacg ccatgaccca ctcgctcgag tcgctcctgt cgcgcaaccc gaacccgttc
     4021 gccgaggcga tcgcgctcgg ggtaatccgg acggtcgccg agtggctccc gcgggccgtc
     4081 gacgacggca cggatctcga ggcgcgcagc cggatgctcc tcgccgccca ctatgccggg
     4141 gtggggcagc agagtggaac cggggtcgga gccgtccatg ccatcggcca cgcgatcgga
     4201 acgcggggcc ggctcgccca cgggaccgcc cttgcgacgg tgatgccgga ggtgttcgcg
     4261 acctacctcg aggtgcgcga gcgggaactt gccctcgtgg cagcggcctt ggggatcgcc
     4321 gacccgcgcg acgagcccgg cgaggtcgcc cgcgccgcga tcgacggcat cgacgccctg
     4381 ctccgatcgg tcggccagcg ccggaccctc gccgagcaag gcctcggtcc ggagacccac
     4441 gagacgatcg cccaggacag catcgaggac gcggcgatca acaacagccc gcggcttccg
     4501 tcgaagcgcg agatcctcgc catcctcgcc gcggtcgccg gctgaacgct ggccgctcgg
     4561 ctgcgttgcc gcccgcgcgg tcaggcggac gcggtgaacg gggcgctcga cgcatcgtcg
     4621 accatcgcgg attcctttcc cttcggatcg cccctcggac cgaacgcatc gtcgcgatgt
     4681 ggcggtccgc gccgggcgcg gcactggctc agcggcggag gaactccggc aggtccggct
     4741 cagggccgcg ccagagggcg agctcctggg tctcgagcca gccgtagtga ttgatgaagt
     4801 gggcgccgtt ggcggtcagg acgtcggcgg ccgcgcccat cgccgtctgg ccacgcacgg
     4861 cgacactgag gacgacgtgg ccctcgcgga cggccgcctc gtaccacgcg aagtcgaccg
     4921 cctggtcggc ccagctgaac tcgaggatct ggaccaggcg cccgcgccag ccgcggcggc
     4981 tgccgctggc gtcgaagacc ggggcatcct cgggccggtc gaaccgctcg atccgggcgg
     5041 cgtcgatcgc cggaagcgag gtcaggcccg cgactgccgc atccgcctct gcgggggtgt
     5101 cgaacacggc gaggagacgg ccgatcgtcg agccgatcat ccgcttcggg cgacgtcctt
     5161 tcacgggctc gcgctcagcc ggcgtcgacg agcggcttga cctcgccgat caggcgctcg
     5221 aacgtctcca tgtcgtaggg cgccggttgc tccgagatga ccgtgtggaa gccgagctcg
     5281 acgtacggcg cgagcttctc ggccaactgc tcggccgtgc cgttccagaa cgtgtcgtcg
     5341 tcggcgacct ccgccagcgg cgtccggttg tgttccatcg ccgccttcca gacacgatcg
     5401 gcctcggcct cggagtcgcg gatcgtggcc ttgatcccga gcgtgaactc gatctcggcg
     5461 gggtcgcggc cgacatcctc gcagtggcgc cgaaggactt cgatcttgtg gcgcatgacc
     5521 tcgaccgggc ccatcgcatt ccacaggtcg gcgtagcggg cgaccgtccg cagggtcttc
     5581 ttctcgcccg agccgccgat catgatcggc agatgggcct ggaccggcgg cggctggtgg
     5641 cgggcgtggt cgaatgcgta gtgcccgccg ggcacggagg tgatctcctt gccgtcgagg
     5701 agggcccgca tcgcggcggc cgactcatcc atccagtcga gtcgctggcc gatgctcgag
     5761 ccgaattcga tgccgtgggc ctcgtgttcg aggtccatcc aggcgccgcc aaggccgagg
     5821 atcgcccgac cgccgctgac gtggtcgagg gtcgcggccg tcttggccac gacacccggg
     5881 ttgcggaacg tgttggcgcc gacgagcagc ccgacgcgga tccgctccgt gctcgccgcc
     5941 gcggcgacca ggctcgacca accctcgaag atcggctggt acggatcgcc gaaaatcgcg
     6001 tagaggtggt cccacgacca gaggtggtcg tagccgagcg cgtccatccg tttgaccgcg
     6061 gccaggtacg acggccagtc ggtggcctgg ttccagagca ggatccccag cttgagatcg
     6121 gtcatcgggt cctccgaggg cggtcagtcg acctcgattc tatggcggat cggccgcccg
     6181 agcgaggagg catcggggcg cgcgaggccg ggcgacgttg cgtgggacgc acgcgtcggc
     6241 cggtcggccg gctggtgatg cccggatctg gtcgacctgt tgagcgccgc gcaaggaatc
     6301 gccgttgtcg tcgtggcgaa cggtgaacgg gaaacccgtt tgcgtcgagc gcaacaacgg
     6361 aggcggatcc ggcgctctgg tcttcgacgt gtgggtgtgg cgaaaggccc tgcagatccg
     6421 ttgcttgtga tgcacggcgg cggctcgtag ggctttgtct gacccgggtt cgacggccgg
     6481 gcccgctatg gcagcgtcgc gatgtcctgg gcgatcggct cgcgatcgtc ggcgtcgacg
     6541 atgccctcga gggcggcggt cgccttcgcc ttccacagcg cacccgacgc cgggtcgccg
     6601 gcggcgaagg acgcccgggc catcgcctcg taggcagccg gcaggtccca gctctcgcgg
     6661 gtgtccgcag ggatcgactc gttgatctcc acgcagcggc gggcatgcca cagcgccggc
     6721 tcgccgcgcc cgagcgtcgc atagacgcgc gagcacagcc actcgccgcg ggcgaggttg
     6781 accggctcgc cgacccgcgc ccaatgccag cgcgacgtgt gcgcccgatg gatcatctcg
     6841 tcggtctcgg cggcggtccg gtcggccttc tcgatcaggg tccaggtgct gttgtagagg
     6901 tcgacgccga gctggcgttg ggtggccgag tcgagctcga cggctgtcgc ggtcgtcgcc
     6961 tcggtggtcg cgttcgacat gatcgggtcc tttccgtcgg tcatccgccc cacgacgtgg
     7021 agcaggcggg tcagtcg
//
LOCUS       DATAIW010000132         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_36152, whole genome shotgun sequence.
ACCESSION   DATAIW010000132 DATAIW010000000
VERSION     DATAIW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36152"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VHM48_04285"
     CDS             <1..1785
                     /locus_tag="VHM48_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2754654.1"
                     /translation="LATMEESEQRLSKTKALAVFSSDALSSVAYATEAILFTLAVAGP
                     EHFWLVLPISGVIVAILFVVAVSYRQTITAYPNGGGSYIVAKENLGTLPGLIAAAALL
                     VDYVLTVSVSVAAGVAALISAFPALENRTLWSLVAIALVMLVNLRGIKESGTIFAIPT
                     YVFVIGMIGLIAVGLGEFALGQSPRVADVTAVASGGQALGALLILRAFADGCSAMTGV
                     EAISNGVPAFKRPEPINARTTLAAMAILLGTMFIGISVLSMLAGSIPSEHETILSQIT
                     RAIFGTGPVYYVLTLATTSILILAANTSFADFPRLASLLAKDRYMPSRFAFRGERLAF
                     SAGIVALAVLAGVVVAAFAGRVEALIPLYAIGVFTCFTLSQAGMVVHWRRDGGPGWRR
                     RATVNGIGAVATAIVALIFAIAKFALGAWIVIIIIPLLVGLMAAIHHEYARASEELDV
                     SERSVIRRPHRRQTAYVPMTDLRRDVVHAVRFGQTLSDEVVGIFVTDDPGAGEALRKR
                     MARQLPGVRLVVVESPYRELVAPLVRYLEQAAKVDPESVSVLLLPEYVQRHWWERFLY
                     NSNQDRIRRALLGNKRILVANVPYRRPA"
     gene            complement(1883..3067)
                     /locus_tag="VHM48_04290"
     CDS             complement(1883..3067)
                     /locus_tag="VHM48_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754655.1"
                     /translation="MTDRDELDVRPSADAMLARIRGDEAALGGRGRLRIYLGMAPGVG
                     KTYRMLEEAHRRVERGTDLVVGYVESHGRPLTAALLDGLEVIPRASVEHRGVVIEEMD
                     TDAVIARAPTLAIVDELAHTNVPGSFRQKRWEDVEAIRDAGIHVISTCNVQHLESVAD
                     AVATITGAPVNERLPDDVLAAADEVELVDMSPRALRQRMKHGNVYPPERAAIALERFF
                     SEGNLTALRELALRFTARRVDEQLGVIATDAGARTGGRFDLVAERTLVVIDGSPGARL
                     ALRRGASLASALRGPLVGLIVETPAGSGASFDASRDLRENLDYAADLGAEIVRLQASD
                     LASGVVEAVRTRRATRVVLAAAPPARGLARLGRRSLLDELLERLPGVELHVVPTGDPG
                     GR"
     gene            complement(3129..>3632)
                     /gene="kdpC"
                     /locus_tag="VHM48_04295"
     CDS             complement(3129..>3632)
                     /gene="kdpC"
                     /locus_tag="VHM48_04295"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX2754656.1"
                     /translation="PAAVTAVAQVVFPHQANGSLISTADGRVIGSSLIGQAFSDPKYL
                     WGRPSGAGQDGYDANGSAGSQLGPTSQALIDRISAEVDRLKAANGDAPIPVDLVTASA
                     SGLDPHISPAAAEYQVARIATARGIDPGLVRAAVARHTDQPLFGFIGEPAVNVLLVNL
                     DLDGLLA"
BASE COUNT          479 a         1336 c         1255 g          562 t
ORIGIN      
        1 ctcgcgacga tggaggagtc ggagcagcgg ctcagcaaga cgaaagccct cgcggtcttc
       61 agcagtgacg ccctctcctc ggtcgcctac gcaaccgagg cgatcctgtt caccctggcg
      121 gtcgccggcc cggagcactt ctggctcgtc ctgccgatct cgggagtcat cgtcgccatc
      181 ctgttcgtcg tggccgtcag ctatcgccag acgatcaccg cgtacccgaa cggcggcggc
      241 agctacatcg tcgccaagga aaacctcggc acgctgccgg gcctgatcgc cgccgcggcc
      301 ctcctcgtcg actacgtcct gacggtctcg gtcagcgtcg cggccggggt ggccgccctg
      361 atcagtgcgt tcccggccct cgagaaccgg accctgtggt cgctcgtcgc gatcgccctc
      421 gtgatgctgg tcaacctgcg ggggatcaag gagtcgggga cgatcttcgc gatcccgacc
      481 tatgtcttcg tcatcgggat gatcgggctc atcgcggtcg ggctcggcga gttcgctctc
      541 ggccagtcac cgcgcgtggc cgacgtgacg gccgtcgcga gcggcggcca ggccctcggt
      601 gcgctgctga tcctgcgcgc cttcgccgac ggctgctccg cgatgaccgg ggtcgaggcg
      661 atcagcaacg gcgtgccggc cttcaagcgc cccgagccga tcaatgcccg gacgacgctg
      721 gctgccatgg cgatcctgct cgggacgatg ttcatcggga tcagcgtcct ctcgatgctc
      781 gccggctcga tcccctccga gcacgagacg atcctgtcgc agatcacccg ggcgatcttc
      841 ggcacgggtc cggtctacta cgtcctgacc ctcgcaacga cgagcatcct catcctggcc
      901 gcgaacacca gcttcgccga cttcccgcgg ctcgcctcgc tgctcgcgaa ggatcgctac
      961 atgccgtcgc ggttcgcctt ccgcggcgag cggctcgcgt tctccgcggg gatcgtcgcg
     1021 ctcgcggtcc tggccggcgt cgtcgtcgcc gccttcgccg gacgcgtgga ggcgctcatc
     1081 cccctgtacg ccatcggcgt cttcacgtgc ttcaccttgt cgcaggccgg catggtcgtc
     1141 cactggcgcc gcgacggcgg cccaggctgg cgcaggcgcg cgaccgtcaa cggaatcggg
     1201 gccgtggcga cggccatcgt cgccctgatc ttcgcgatcg cgaagttcgc cctcggcgcc
     1261 tggatcgtga tcatcatcat cccgctgctc gtcgggctga tggccgcgat ccaccacgag
     1321 tacgcgcgcg cctccgagga gctcgacgtc agcgagcgct cggtcatccg ccggccgcac
     1381 cgacgacaga ccgcctacgt cccgatgacg gacctccggc gcgacgtcgt gcacgcggtt
     1441 cgcttcggcc agaccctttc cgacgaagtc gtcggcatct tcgtcaccga cgatcccggg
     1501 gccggggagg cgctccgcaa gcgcatggcc cgccagctgc ccggcgtccg gttggtcgtc
     1561 gtcgaatcgc cctaccgcga gctcgtcgcg ccgctcgttc gctatctcga gcaggcggcg
     1621 aaggtcgacc ccgagtcggt ctcggtcctg ctcctgcccg agtacgtcca gcggcactgg
     1681 tgggagcgct tcctgtacaa ctcgaaccag gaccggatcc gccgcgccct gttgggcaac
     1741 aagcggatcc tggtcgcgaa cgtcccgtac cgccggcccg cctgacgccc tccggccggc
     1801 gttcgccttc gcgtaccaac ggccgacccg gacgtcagcg atgagatggt ccgaggtggc
     1861 cgggtgcgcg tcgcggacct cgtcagcggc cgcccggatc cccggtcgga acgacgtgga
     1921 gctcgacgcc cggcagccgc tcgagcagct cgtcgagcag ggaccgccgg ccgagccggg
     1981 cgagaccccg ggccgggggt gccgccgcca gcacgacccg cgtcgcccgt cgcgtccgga
     2041 cggcctcgac gacgccgctg gccaggtcgg acgcctggag ccggacgatt tcggccccga
     2101 ggtccgcggc gtagtcgagg ttctcgcgga ggtcccgtga cgcatcgaac gacgccccag
     2161 atccggccgg agtctccacg atgagtccga ccagcggtcc acgaagtgcc gacgccagcg
     2221 aggcgccgcg ccgcagcgcg aggcgcgcgc cgggcgaccc atcgatgacg acgagcgtcc
     2281 gttcggccac gaggtcgaac cgtccgcctg tccgggcgcc cgcatcggtg gcgatcacgc
     2341 cgagctgctc gtcgacccgg cgcgcggtga accggagagc cagctcgcgg agggcggtca
     2401 ggttgccctc gctgaagaac cgctcgagcg cgatcgcggc ccgctccggc ggatagacgt
     2461 tgccgtgctt catccgctgg cggagggccc gcgggctcat gtcgaccagc tcgacctcgt
     2521 cggccgcggc gaggacgtcg tccggcagcc gctcgttgac gggtgctccg gtgatcgtgg
     2581 cgaccgcgtc ggcgacgctc tcgagatgct ggacgttgca ggtgctgatg acatggatgc
     2641 ccgcgtcacg gatcgcctcg acatcctccc agcgtttctg gcggaacgag cccgggacgt
     2701 tcgtgtgggc gagctcgtcg acgatcgcga gcgtcggcgc ccgggcgatc accgcgtcgg
     2761 tgtccatctc ctcgatgacg acgccgcgat gctcgacgct ggcccgcggg atcacctcga
     2821 ggccgtcgag cagcgcagcg gtcagcggtc gcccatgcga ctcgacatat ccgacgacga
     2881 gatccgtgcc gcgctcgacc cgccgatgag cctcctcgag catccggtag gtcttgccga
     2941 cgcccggcgc catcccgaga tagatccgga ggcgaccgcg gccgcccagc gccgcctcgt
     3001 cgccccggat ccgggcgagc atggcgtcgg cgctcggccg gacgtcgagc tcgtcgcgat
     3061 cggtcaccgg tgatgccctt tctgccccgt gcgagcggcc gcgctcgccg cgtcggggtt
     3121 ccgcccgatt atgccagcag gccgtcgagg tcgaggttga cgagcaggac gttgacggcc
     3181 ggctcgccga tgaagccgaa cagcggctgg tcggtgtggc gggcgaccgc cgcccggacg
     3241 aggcccgggt cgattccgcg ggccgttgcg atccgggcga cctggtactc cgcggccgcc
     3301 gggctgatgt gcggatcgag cccggacgcc gaggcggtca cgagatcgac cgggatcggc
     3361 gcgtcgccgt tggcggcctt caggcggtcg acctcggcgc tgatccggtc gatcagggcc
     3421 tggctcgtcg gcccgagctg cgacccggcg gagccgttcg cgtcgtagcc gtcctggccg
     3481 gcgccggacg gccggcccca caggtacttc gggtcgctga acgcctgccc gatcaggctc
     3541 gagccgatca cccggccatc ggcggtcgag atcagcgacc cgttcgcctg gtgggggaag
     3601 accacctggg cgacggccgt cacggccgcc gg
//
LOCUS       DATAIW010000133         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_36213, whole genome shotgun sequence.
ACCESSION   DATAIW010000133 DATAIW010000000
VERSION     DATAIW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36213"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="truB"
                     /locus_tag="VHM48_04300"
     CDS             complement(<1..758)
                     /gene="truB"
                     /locus_tag="VHM48_04300"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2754657.1"
                     /translation="MSLDGVLVVAKPVGPTSHDVVGLVRRLAATKRVGHGGTLDPFAS
                     GVLPVFLGKATRLVEYHLGDRKRYRATVCFGASSTTDDLEGALTRASGPVPSRAAVEA
                     ALAAFRGPIEQRPPAYSAIKVAGRRAYAMARAGEAVELAPRAVTIHALDIIGWDETTA
                     GEPVAIVEVECSAGTYVRSLARDLGEAVGSAAYLGALTRTASGPFGLLAATDLDAIRT
                     AAADGPAAIAALLLPIDAGLDLPTVIVPDAALVA"
     gene            complement(772..1788)
                     /locus_tag="VHM48_04305"
     CDS             complement(772..1788)
                     /locus_tag="VHM48_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX2754658.1"
                     /translation="MIDLTPWLDAVPDRVLERLTAARRVLAVGHENPDADTLGATLAI
                     CRLAERGGATATAVFADPVPPLYDFLADVARARTDPDPAIDYDLLVVSDCGSLDRIGG
                     VRGRHAELFERLPRVIIDHHASNDAAGDADWIDPGSAATCEMVTLLAARMGAPLGDGD
                     GALAADLMAGIVMDTATFAHPNATPRTLAVAAALVEAGAPLSDISRRLYRSKPDAQLK
                     LFGRVLDRLETAAGGRIIFSTLLDEDLAATGAIAPHSEGIIDLLAQSELAEIAIVFKE
                     AGEGTRVSVRTKPGGVDATVLTGRFGGGGHARASGATVPLPVAEARRAVLAEAERMVA
                     AVAR"
     gene            complement(1785..2345)
                     /gene="rbfA"
                     /locus_tag="VHM48_04310"
     CDS             complement(1785..2345)
                     /gene="rbfA"
                     /locus_tag="VHM48_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX2754659.1"
                     /translation="MTARTDRIDELFRQEITSILSREVDDPKIGFATITGVDTQPDLR
                     HAKVKVSVIGPLEERRATIVAMGRHMPFVRHELGKRLRMKRIPEFHLELDETLERGTR
                     VLHLLDELGAGHIPEDVAPDGETLPTPTPLIPTDRSITEGLGGAAADLASAAARESAA
                     REAGQPRRTFRPGSRTNRTVPGKRRR"
     gene            complement(2397..4301)
                     /gene="infB"
                     /locus_tag="VHM48_04315"
     CDS             complement(2397..4301)
                     /gene="infB"
                     /locus_tag="VHM48_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2754660.1"
                     /translation="MARSRSGTRGRRGPRKPPRRPGDANSQVQTIDPRERGAIELPTT
                     ITVKELAELLAVNPADVIRELIKSGIFATINQLIDRDTASLVAGELGYEVAETIAPTA
                     GVAVEGEEERAPAPAAKEVLFDADVEADLLPRAPIVTVMGHVDHGKTSLLDAIRTTAV
                     AAGERGGITQHIGASEVERNGKRVVFLDTPGHEAFTAMRARGAKVTDIAVVVVAADDG
                     VMPQTLEAISHAKAAKVPIIIALNKIDRADANQDRVKTELSEAGVIVEDYGGDTPLVP
                     VSAKTRVGLDELIEMILLVADLQELKANPKRAAVGTIVEAQLDKNRGAIATAIVQTGT
                     LRVGDVIVVGETFGKVRALENDEGKRITKAGPSSAVVVLGLSAVPEAGDILRVVSDEK
                     EARSMVEARKAEAAAKSGEGSTRATLEDLYRQIQAGQAKELRIILKADVSGSLGAITH
                     ALDQLNQDEIRINVLHEGAGDITDNDIMLASASDAIIVGFSTKITETARRAADAEGVD
                     VRLYDIIYKLTDDISAALQGLLEPEIVEVIEGRAEVRQIFRVGKSTVIAGCFVTDGRI
                     VRGGARVWRNGKIAVTDKIESLRRFRDDVREVERNYECGIGLAGYNDLEEGDIIECFT
                     SQSVSRVLPS"
     gene            complement(4288..4653)
                     /locus_tag="VHM48_04320"
     CDS             complement(4288..4653)
                     /locus_tag="VHM48_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX2754661.1"
                     /translation="MASRSSASDLGERVPARRSGSPVHARVPTRSCVACRTARPKRDL
                     VRVVRTPDGQMIMDETGRLAGRGAYLCRDAGCWTLAAERGALGRALGAAVPPELRAAL
                     LAGPAMMTMTERGGARGQE"
     gene            4684..>4870
                     /locus_tag="VHM48_04325"
     CDS             4684..>4870
                     /locus_tag="VHM48_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754662.1"
                     /translation="MAVAFFARVFFAFAGAASVAAAASSAAGSVAAFAVGFFARVFGA
                     APAKAKKTRAKKATATEE"
BASE COUNT          637 a         1728 c         1769 g          736 t
ORIGIN      
        1 atcgcgacga gcgccgcgtc cgggacgatc acggtcggca ggtccagacc ggcgtcgatc
       61 ggcaggagga gcgccgcgat cgccgccggc ccgtcggccg cggcagtccg gatcgcgtcg
      121 aggtcggtcg cggccaggag cccgaacggg ccgctcgccg tccgggtcag cgcgccgaga
      181 tacgcggcgc tgccgaccgc ctcgccgaga tcccgggcga gcgaccggac atacgtgccc
      241 gccgagcact cgacctcgac gatcgcgacc ggctcgccgg ccgtcgtctc gtcccagccg
      301 atgatgtcga gggcatggat cgtgaccgcc cgcggagcga gctcgaccgc ctcgccggcg
      361 cgagccatcg cgtaggcccg ccggccggca accttgatcg cgctgtaggc gggcggccgc
      421 tgctcgatcg gtccacggaa ggcggccagg gccgcctcga ccgccgcccg gctcggcacc
      481 ggaccggagg cgcgcgtcag ggcgccctcg aggtcgtcgg tcgtcgacga cgccccgaag
      541 cagacagtcg cccggtagcg cttgcggtcg ccgaggtggt actcgacgag ccgggtcgcc
      601 ttgccgagga acaccggcag gacgcccgag gcaaacggat cgagcgtccc gccgtgcccg
      661 acccgtttgg tggccgccag ccggcggacc aggccgacga cgtcgtgcga ggtcggcccg
      721 accggcttgg cgaccacgag gacgccgtcg aggctcatcg gcaggatgcg ctcaacgggc
      781 gaccgcggcg accatccgct ccgcctcggc gaggacggcg cggcgagcct cggcgaccgg
      841 caacgggacg gttgcgcccg aggctcgcgc gtggccgccg ccaccgaacc ggcccgtcag
      901 gaccgtggcg tcgacgcccc cgggcttggt ccggacgctc acccgggtcc cctcccccgc
      961 ctccttgaac acgattgcga tctcggcaag ctcggactgg gcgaggaggt cgatgatccc
     1021 ctcagagtgc ggcgcgatcg cgccggttgc ggcgaggtcc tcgtcgagca gggtcgagaa
     1081 gatgatccgc ccgccggcgg ccgtctcgag ccgatcgagg acccgtccga acagcttcag
     1141 ctgggcgtcc ggcttggacc ggtacaggcg gcgcgagatg tccgagagcg gcgcgccggc
     1201 ctcgacgagg gcagccgcca cggccagcgt ccgcggggtg gcgttcgggt gggcgaaggt
     1261 cgccgtgtcc atgacgatgc cggccatcag gtcggcggcc agggcgccgt cgccgtcgcc
     1321 gaggggcgct cccatccggg cggcgaggag cgtcaccatc tcgcaggtcg ccgcgctccc
     1381 ggggtcgatc cagtcggcgt cacccgcggc atcgttggag gcgtggtgat cgatgatgac
     1441 ccgcggcaat cgctcgaaca gctccgcatg gcggccccgg acgccgccga tccgatcgag
     1501 ggagccgcag tccgagacga cgagcaggtc gtagtcgatc gccgggtcag ggtccgtcct
     1561 ggcgcgggcc acatcagcga ggaagtcgta gagcggcggc accgggtcgg cgaagacggc
     1621 ggtcgccgtg gccccgccac gttcggccag gcggcagatc gcgagggtcg cgcccagcgt
     1681 gtcggcgtcc ggattctcgt ggccgacggc gaggacgcgt cgggcggccg tcaggcgttc
     1741 gagcacgcgg tccggaaccg cgtcgagcca cggcgtgaga tcgatcatcg acggcgcttg
     1801 ccgggaacgg tccggttcgt ccgcgacccg ggtcggaacg tgcggcgcgg ttgaccggcc
     1861 tcgcgggccg cggactcgcg agccgccgca ctcgcgagat cggccgccgc gccgccgagg
     1921 ccctccgtga tcgagcgatc cgttgggatg agcggcgtcg gcgtcgggag tgtctcgccg
     1981 tcgggagcca cgtcctctgg aatgtggccg gcgccgagct cgtcgagcag gtggaggacg
     2041 cgcgttcccc gttccagcgt ctcgtcgagc tccaggtgga actccgggat gcgcttcatc
     2101 cggagccgct tgccgagctc gtggcggacg aacggcatgt gccgacccat cgcgacgatc
     2161 gtcgcccgcc gctcctcgag gggcccgatc acgctcacct tgaccttcgc gtgccgaaga
     2221 tccggctgcg tgtcgacccc ggtgatcgtg gcgaacccga tcttcggatc gtcgacctcg
     2281 cggctgagga tcgacgtgat ctcctgccga aagagctcat cgatccgatc cgttctcgcg
     2341 gtcatcgggc caccagcgcg cggcggagcc agctcagccg tgccatcgga ctcgaatcag
     2401 ctcggaagaa cgcgcgatac cgactgcgag gtgaaacact cgatgatgtc tccctcttcc
     2461 aggtcgttgt agccggccag gccgatgccg cactcgtagt tccgctcgac ctcacggacg
     2521 tcgtcgcgga accggcggag cgattcgatc ttgtcggtga cggcgatctt gccgttgcgc
     2581 cagacccggg cgccgccgcg gacgatccgc ccgtcggtga cgaagcagcc ggcgatgacc
     2641 gtgctcttgc ccacccggaa gatctggcgg acctccgctc gaccctcgat gacctcgacg
     2701 atctccggct cgagcaggcc ctggagggcc gccgagatgt cgtcggtcag cttgtagatg
     2761 atgtcgtaca ggcggacgtc gacgccttcc gcatccgcgg ctcggcgggc ggtctcggtg
     2821 atcttcgtgc tgaagccgac gatgatcgcg tccgaggcgg aggcaagcat gatgtcgttg
     2881 tcggtgatgt ccccggcgcc ctcgtggagg acgttgatcc ggatctcatc ctggttgagc
     2941 tggtcgaggg cgtgggtgat cgcaccgagc gagcccgaca cgtcggcctt gaggatgatc
     3001 cgcagctcct tggcctggcc ggcctggatc tggcggtaga ggtcctcgag ggtcgcccgg
     3061 gtgctgcctt cgccgctctt ggcggccgct tcggccttgc gggcctcgac catcgagcgg
     3121 gcttccttct cgtcggacac gacgcgcagg atgtcgcccg cctcgggcac ggccgacagg
     3181 ccgaggacca cgacggccga cgacgggccc gccttggtga tccgcttgcc ttcgtcgttc
     3241 tcgagcgccc ggaccttgcc gaacgtctcg ccgacgacga tcacgtcgcc gacccggagc
     3301 gtcccggtct ggacgatcgc cgtcgcgatc gcgccgcggt tcttgtcgag ctgggcctcg
     3361 acgatcgtgc cgacggcggc ccgcttcggg ttcgccttca gctcctggag gtcggcgacg
     3421 agcaggatca tctcgatcag ctcgtcgagc ccgacgcggg tcttggcgga cacggggacg
     3481 agcggcgtgt cgccgccgta gtcctcgacg atgacgcccg cctcggacag ctcggtcttg
     3541 acccggtcct ggttggcgtc ggccctgtcg atcttgttga gggcgatgat gatcggcacc
     3601 ttggccgcct tggcgtggct gatcgcttcg agcgtctggg gcatgacccc gtcgtcggcg
     3661 gcgacgacga cgaccgcgat gtcggtcacc ttcgccccgc gggcgcgcat cgcggtgaac
     3721 gcctcgtggc ccggcgtgtc gaggaagacg acccgcttgc cgttgcgctc gacttcgctc
     3781 gcgccgatgt gctgggtgat cccgccgcgc tcgccggccg cgaccgcggt cgtccggatc
     3841 gcatcgagga ggctcgtctt gccgtggtcg acgtggccca tgacggtcac gatcggggcg
     3901 cgcgggagca ggtccgcctc gacgtcggcg tcgaagagga cctccttggc ggccggcgcc
     3961 ggggcccgtt cctcctcgcc ctcgacggcc acaccggcgg tcggggcgat cgtctcagcc
     4021 acttcgtagc cgagctcgcc ggcgacgagc gacgcggtgt cgcgatcgat cagctggttg
     4081 atcgtggcga agatcccgct cttgatcagc tcgcggatca cgtccgccgg gttgaccgcg
     4141 aggagctcag cgagctcctt gacggtgatc gtcgtcggca gctcgatcgc gccacgttcg
     4201 cgcggatcga tcgtctggac ctgtgaattg gcgtcgccgg gacgacgcgg tggcttgcgg
     4261 ggcccgcgcc ggccgcgggt gccgctccta ctcctggcca cgggctcctc ctcgttctgt
     4321 catcgtcatc atcgccggtc cggccaggag cgccgcgcgg agctccgggg ggacggctgc
     4381 cccgagcgcg cgcccgaggg cgcctcgctc ggcggcgagg gtccagcagc cggcgtcccg
     4441 gcagaggtag gcacccctgc cggccagccg gccggtttcg tccatgatca tctgcccgtc
     4501 cggcgtccgg accacgcgga cgaggtcacg cttggggcga gccgtccggc aggcaacgca
     4561 cgatcgggtc gggacccgcg cgtgaaccgg cgaccccgac cggcgagcgg gaacccgctc
     4621 accgaggtcg gaggcggagc tccgcgacgc catccgcccg aggtttacga ccccacctcc
     4681 tcggtggcgg tcgccttctt cgcccgcgtc ttcttcgcct tcgccggggc agcttcggtc
     4741 gccgccgcgg catcgtccgc cgcgggctcc gtggccgcct tcgcggtcgg cttcttcgcc
     4801 cgggtcttcg gagctgcccc ggcgaaggcg aagaagacgc gggcgaagaa ggcgaccgcc
     4861 accgaggagg
//
LOCUS       DATAIW010000134         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_66102, whole genome shotgun sequence.
ACCESSION   DATAIW010000134 DATAIW010000000
VERSION     DATAIW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66102"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1063
                     /locus_tag="VHM48_04330"
     CDS             344..1063
                     /locus_tag="VHM48_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2754663.1"
                     /translation="MTRAFVDLHTHSRASFDSLAVPASLVRAAASRGLTHLAITDHDR
                     IDGALEGRERAAETDPGLTVIVGEEVRTADGDLICLFLEQAIPPGLPAEEAIARARDQ
                     GALVGVPHPFDRFRGSLLRDARMERLAGLVDWIETHNARVAVGKGNQLAAEFAVEHGL
                     PGIAVSDAHSSFEVGVAYTVLDGDPSTAAGLLAALSSAELMTGRASMYVRLLTPLAKV
                     VQRARGNGRIEPTGDAPAAIR"
     gene            1060..2331
                     /locus_tag="VHM48_04335"
     CDS             1060..2331
                     /locus_tag="VHM48_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2754664.1"
                     /translation="MSGLPGATGPRGGAGSDAERRSGDERRTGEERRDASPPIETASP
                     PIETASTVPATPPTEDDRRSGAERRSDLDRRTNETVTADQLSLGRRLRQRRTIVSLVL
                     PLLLLVLFARALPGFKLDQVPGLILQANPLLLFGAFAVFYSGFPLRGLRWAILIRATG
                     FPLRVRDATEIIFISWLVNCLVPAKLGDLYRAYLLKINSTVSLSRTFGTVFIERILDL
                     FAIVTLGLASGYWSFRGRLPGDVQLVFAVGVGVVVLLAGGLLTMRNFGRRLMTALPLP
                     HRVTEFYDRFEEGVFSAVGLRALPRLIVLTALVWATEAMRLFLVVEALGFKDVHLGIS
                     GAFFVALTGSLLTAVPLTPAGIGVVEAGVVFVLTVVYNIDQPQALAIVLVDRVISVLS
                     IIGFGSLAYWFSPKRRGMGVDQHRPVEVSSA"
     gene            2728..3270
                     /locus_tag="VHM48_04340"
     CDS             2728..3270
                     /locus_tag="VHM48_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2754665.1"
                     /translation="MTTQNGRAFNEWLRAQLKAKKMSQRQLAQQSGVDHSTISRLIRG
                     DRMPSLGTATKLARGLRELRDDADTPQYLGLVASGATNPTARVEYALRADELLSEPQV
                     RQIMEYYLAVRMRRFGRAFQPSEPAASRSPERPIARSAPVGQGPVVAGVGQASAGPSN
                     SSRMGLVRPMGRNLGRPGRP"
     gene            complement(3556..>4073)
                     /locus_tag="VHM48_04345"
     CDS             complement(3556..>4073)
                     /locus_tag="VHM48_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754666.1"
                     /translation="LARLLARPTRGANLVTAVGAAGVIVALVGWNLASQPPSRASDGG
                     WEAARAAAQRVLVSGGTGPLVLSSLPVFKSDEALRMPLQADGVELTPAGPNGASYADP
                     ETPRVILCDQLFRDAIGADCGGPAEDAWIVDPASGLRLGAAAPAGAAPTLLDRFEAAP
                     GRWVSIYRQQP"
BASE COUNT          529 a         1501 c         1400 g          643 t
ORIGIN      
        1 gtctaggagg ttcgatggcc ggggcgggcg cggtgttgcc gttcaccatc gcgggtggtg
       61 cgcggcagcg tgtggccccg gtcgcgtgtc gggtttggcc atcgaccacc cccggtggtg
      121 cgcgtcacgt tcttgcccca gccgcgctcc tcgtttcggc atttaccacg gcgggtggtg
      181 ggcgtcgctg cgctcgcgtc tgcggagcct gttcggtgcc cactgtgcgt cgctcacggc
      241 caaccgacac cccagtcgac gcttcaatcg gttcgagctc gcgacttgca ccacgccgag
      301 tggtatctgg cggaccctgg cccgtgccga cgtcggaagc ctgatgaccc gcgccttcgt
      361 cgacctccac actcattcgc gggcgtcgtt cgattcgctt gccgtaccgg cgtcgctcgt
      421 ccgggcggcg gcgagccgtg gcctgaccca tctcgcgatc accgatcacg accggatcga
      481 cggcgccctc gagggtcgcg agcgggcggc ggagacggat ccgggcctca cggtgatcgt
      541 cggcgaggag gtccggacag cggacggtga cctgatctgc ctgttcctcg agcaagcgat
      601 cccgccgggc ctgccggccg aggaggcgat cgcccgggcc cgggatcagg gcgccctcgt
      661 cggtgtcccg catccgttcg accggttccg tgggtcgctc ctccgcgatg cccggatgga
      721 gcggctggcc gggctggtcg actggatcga gacccacaat gcgcgggtcg cggtcggcaa
      781 gggcaaccag cttgcggccg aattcgcggt cgagcacgga ctgcccggga tcgcggtgtc
      841 ggacgcgcac agctcgttcg aggtcggtgt cgcctatacg gtcctcgacg gagacccgtc
      901 gaccgcggcc ggcctcctcg ccgcgctctc gagcgcggag ctcatgaccg gccgcgcatc
      961 gatgtacgtc cggctcctga cgccgctggc caaggtcgtc cagcgagctc gcggcaacgg
     1021 ccggatcgaa ccgacgggtg acgcgccggc cgccatccga tgagcggcct gcccggcgcg
     1081 accggtccac gcggcggcgc cggatccgac gccgagcgcc gcagcggcga cgagcggcga
     1141 accggcgagg agcgccgcga cgcgtcgccg ccgatcgaga ccgcgtcgcc gccgatcgag
     1201 accgcttcga ccgtcccggc gacaccgcca accgaagatg accggcgcag cggcgccgaa
     1261 cggcgatccg atctcgaccg gcgcacgaac gaaacggtca ccgccgacca gctatcgctg
     1321 ggacgccgtc tccgccagcg acggacgatc gtgtcgctcg tcctgccgct gctcctgctg
     1381 gtcctctttg cccgtgccct gcccggcttc aagctcgacc aggtgccggg cctgatcctc
     1441 caggccaacc cgctcctgct gttcggcgcg ttcgccgtgt tctactccgg cttcccgctc
     1501 cggggcctgc gctgggcgat tctgatccgg gccaccgggt tcccccttcg ggtccgggac
     1561 gcgaccgaga tcatcttcat cagctggctc gtcaactgtc tcgtgccggc caagctcggc
     1621 gatctctatc gggcgtacct gctcaagatc aacagcacgg tctcgctgtc gcggacgttc
     1681 gggaccgtct tcatcgagcg gatcctcgac ctcttcgcga tcgtgaccct cggcctggcg
     1741 tcgggctact ggtcgttccg cggccgcctg cccggcgacg tccagctcgt gttcgccgtc
     1801 ggcgtcggcg tcgtcgtcct gcttgccggc ggcctcctga cgatgcgcaa cttcggccgg
     1861 cggctgatga cggcgctgcc gctgccgcat cgggtgaccg agttctacga ccggttcgag
     1921 gagggcgtct tctcggccgt cgggctgcgt gcgctgccgc ggctcatcgt cctcaccgcc
     1981 ctcgtctggg cgacggaggc gatgcgactc ttcctcgtcg tcgaggccct cggtttcaag
     2041 gacgtccacc tcggcatcag cggcgcgttc ttcgtcgcgc tgaccggctc cctgttgacc
     2101 gcggtgccgc tcacgccggc cgggatcggg gtggtcgagg ccggcgtcgt gttcgtcctg
     2161 accgtcgtct acaacatcga tcaaccgcag gcgctggcga tcgtcctcgt cgatcgggtg
     2221 atcagcgtcc tctcgatcat cggcttcgga tcgctggcct actggttctc gccgaagcgg
     2281 cgcgggatgg gcgtcgacca gcaccgaccg gtcgaggtct cctcggcctg aagcgcgagc
     2341 gggggatcgg cacacatccg caacgcgttc ggggcatccg cgcgcgccaa catgccgccc
     2401 gatgaccgtt catgacagta tggcaagccg tgcccaggtc cccgtcctgc tccgatcgcg
     2461 tgatccgcgc cgaacggccg cgtgttgcct cgacgccgat cccatccgtc cgcgaccggc
     2521 cgacgtagcc cctggtgcgg gacggcagtc ccctgcatgc tccggtccgc acccccttgc
     2581 accccaccgg actggtcggc ccgcgaggtt tctctcgccg accggggtgg taccaacggg
     2641 tgatacagga ggggccggtc cgttcgtgat catgcagcta tcacggcatc acccgactgc
     2701 gcgagacacg gagcgcccaa cgacgtcatg accacccaga acggtcgagc gttcaacgaa
     2761 tggctgcgag cccagctcaa ggccaagaag atgtctcagc gtcagctcgc ccagcagtcg
     2821 ggtgtcgacc actcgaccat ctcgcgactg atccgcggcg atcgcatgcc gtccctcggc
     2881 accgccacga agctcgctcg agggctgcgt gagctgcgcg acgacgccga cacgccgcag
     2941 tacctcggtc tcgtcgcctc cggggcgacg aacccgaccg cccgggtcga gtacgcgctg
     3001 cgggccgacg agcttctgag cgagccccag gtccgccaga tcatggagta ctacctcgcc
     3061 gtccggatgc gtcgcttcgg ccgcgcgttc cagccatccg agcccgcggc cagccgttcg
     3121 ccggagcgcc cgatcgcgcg ctctgccccg gttggccagg ggcccgtcgt ggccggggtc
     3181 ggacaggcga gcgcgggccc ctccaattcg tcgcgcatgg ggctggtcag gccgatgggc
     3241 cgcaacctcg gccgaccagg gcgaccctag gcaggtcgcc ggcctcggac gaggccacgc
     3301 caaccacatc cttctccagg gcacgactcg gacgccctcc acgtccgggt ctcggggaca
     3361 gcgaacgccg gcatctgggg tgccggcgtt cgcgattcac gtgacgcagt cgacggctgt
     3421 cgcgcctggg cacgccatcg ccgcgttgcc ggctgcgctc ctcgcttacg cacctgcggg
     3481 tgcgctcgct ccggtgctcg ccggcgcttg cgccgacgcg ccccggcacg acgaacgcgg
     3541 tcgcgtgcct gcgtgtcagg gttgctggcg gtagatcgag acccagcggc cgggggcggc
     3601 ttcgaaccga tcgagcaggg tgggggcggc gccggccggc gccgcggcgc ccaggcggag
     3661 gcccgacgcc gggtcgacga tccaggcgtc ctcggccggt ccaccgcaat cggcgccgat
     3721 cgcgtcgcgg aagagctggt cgcacaggat gacccggggc gtctccggat ccgcgtagga
     3781 cgccccgttc gggcccgccg gagtgagctc gacaccatct gcctggagcg gcatccggag
     3841 cgcctcatcg ctcttgaaca ccggcaggct cgagaggacc agcgggccgg taccgccgga
     3901 gacgaggacg cgctgagcgg ccgcccgggc cgcctcccag ccgccatcgc tggccctcga
     3961 cggtggctgg ctcgcgaggt tccagccgac gagggcgacg atcaccccgg ccgcgcccac
     4021 cgcagtgacc aggttggctc cgcgggtggg ccgcgcgagg agtctcgcca gcc
//
LOCUS       DATAIW010000135         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_95776, whole genome shotgun sequence.
ACCESSION   DATAIW010000135 DATAIW010000000
VERSION     DATAIW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95776"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..922)
                     /locus_tag="VHM48_04350"
     CDS             complement(47..922)
                     /locus_tag="VHM48_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2754667.1"
                     /translation="MDHSEVRELLEDAAIEPRGLERLMAGDTPAASIVAGHLAGCFDC
                     AEELERLRRSVEVIGPTVRAVPSPELRERTLAYVAALGRPRGSAPDATMATAPAPALS
                     PPPMLAPVPVANPVSAAGSRTREPDRRNRLGWLASMAAALIVAVTGTALLVNANHDSQ
                     TGVLSAEIDALGDVARWTLRVDAQSDVRRIQLASTTGAATSASLIYSPTSTELVIVAD
                     QLAPPAAGQEYSCWVEVGGTRTRIGKMFFGGDLAYWVGKVPQVAGVAPDARFGVSLVG
                     PGAPAAPGDRILVSG"
     gene            complement(931..1545)
                     /locus_tag="VHM48_04355"
     CDS             complement(931..1545)
                     /locus_tag="VHM48_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754668.1"
                     /translation="MALGELYDRYVDAVFAAASRLTSDRQLAEEVVQETFLALWNRAE
                     LFNPALGSLAAWLHTIARNRTVDRLRAAGRRPNLIPLSSAAGEDEQAGAALDRLVAAG
                     NVIGGATVGRGPEGELAASELQHVLRDALAELPDTERTAIVLAYREELTQTEIAERLG
                     WPLGTVKTRTRRALLRLREALAEELGPGVAPTPIAVPIHDGMER"
     gene            complement(1614..2099)
                     /locus_tag="VHM48_04360"
     CDS             complement(1614..2099)
                     /locus_tag="VHM48_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2754669.1"
                     /translation="MSERISTNDAHILDVLQRDGRRPYADLGGDVGMSGPSAHERVKK
                     LEARGVITGYAAIVDPRTLGYDILAFSWVTQAPGTVAHDLTDSFAAIPEIEECHHITG
                     EADYLIKVRARDTRDLERVLRRVQSTQDVFSTETDVVFSSGFERRPLHIPAETDDASG
                     T"
     gene            2259..2390
                     /locus_tag="VHM48_04365"
     CDS             2259..2390
                     /locus_tag="VHM48_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754670.1"
                     /translation="MFAIGLGFLVLFSTLSILLGTEDSHHSTDPRDTLAIWARVGAR"
     gene            complement(2627..2764)
                     /locus_tag="VHM48_04370"
     CDS             complement(2627..2764)
                     /locus_tag="VHM48_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEX2754671.1"
                     /translation="MRHRTGGSDEATVIVNWLKTDHGLGHAMAIVQLIKQARAQAQAQ
                     A"
     gene            complement(2782..3093)
                     /locus_tag="VHM48_04375"
     CDS             complement(2782..3093)
                     /locus_tag="VHM48_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754672.1"
                     /translation="MMDVAARIRAEGLDPSSWSNGPGDRYGAHDHGYDKVIVVASGSI
                     AFGLPDDGGSVALDVGDRLELPAGTRHDATVGPKGVTCLEAHLPAGRLPAVVRRPAGE
                     W"
     gene            complement(3090..>3872)
                     /locus_tag="VHM48_04380"
     CDS             complement(3090..>3872)
                     /locus_tag="VHM48_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2754673.1"
                     /translation="DEREHAEIWRKLKDPDYHPDRDIAAREKWHRSGRSGTLRAVIFG
                     VSDGLVSNLALVMGVAGASGPTATAGHFILLAGVAGLLAGAFSMAAGEYISMQSQREL
                     FERQIALERAEMEAMPEEEEAEMAALYRAKGFPADEAKAIAHRLFDDPERALDQLIRE
                     ELGLDPDELGSPFGAAFGSFIAFALGAFVPVVPYLVGGGVGAFVAAIGLSLVALFGVG
                     AGVSLLTGRSVLFSGARQVGIGAAAAIVTYVVGTIIGVSVAG"
BASE COUNT          581 a         1360 c         1351 g          580 t
ORIGIN      
        1 tcacgagggc ggaccggcct gacgccgtcg ccggagccga cccgagtcag ccgctgacga
       61 ggattcggtc gccgggcgca gcgggcgcgc cgggacccac gagcgaaaca ccgaatcgag
      121 cgtcgggcgc cacgccggcg acctgcggga ccttgccgac ccagtaggcg agatcaccac
      181 cgaagaacat cttgccgatc cgcgtccgcg tgccgccgac ctccacccag cagctgtact
      241 cctggccggc cgccgggggt gccagctggt cggcgacgat gacgagctcg gtcgaggtcg
      301 gcgaataaat gaggcttgcg ctcgtggccg ccccggtcgt gctcgcaagc tggatccggc
      361 ggacgtcgga ctgggcatcg acccgcagcg tccagcgggc gacgtcgccg agcgcatcga
      421 tctcggcgga caggactccc gtctgcgagt cgtgattcgc gttgacgagc agggcggtcc
      481 cggtcacggc gacgatcagc gcggctgcca tgctggcgag ccagcccagg cggttgcggc
      541 ggtcaggctc gcgggtccgg gaaccagccg cgctcaccgg gttggcgacg ggcacgggcg
      601 cgagcatcgg tggcggggac agagccgggg ccggggccgt ggccatggtg gcatcgggcg
      661 ccgatccgcg cggccggccg agcgccgcca cgtaggccag cgttcgttcc ctcagctccg
      721 gcgacgggac cgcgcggacc gttggcccga tcacctcgac cgatcgacgg agccgttcga
      781 gctcctcggc gcaatcgaag cagcccgcga gatgcccggc gacgatcgac gccgccggcg
      841 tgtcgcccgc catgagtcgc tcgagcccgc gcggctcgat cgccgcatcc tcgagaagct
      901 cgcgcacctc gctgtgatcc atcgctccca tcatcgttcc attccgtcgt ggatcggcac
      961 cgcgatcggc gtcggcgcca ctcccggacc gagttcctcg gccaacgctt ccctcagtcg
     1021 aaggagggcc cgccgcgtcc tcgtcttgac ggtcccgagc ggccagccga gccgttcggc
     1081 gatctccgtc tgagtcagtt cctcgcgata cgccaggacg atcgcggttc gttcggtatc
     1141 gggcagctcg gccagcgcgt cccggagcac gtgctgcagc tcactcgccg caagctcgcc
     1201 ttccgggccg cgtccgaccg tggcgcctcc gatcacgttg ccggcggcga cgagccggtc
     1261 gagtgcggcc ccggcctgct cgtcctcgcc ggccgccgac gagagcggga tcaggttggg
     1321 acggcgaccc gccgcgcgca gccggtcgac cgttcggttg cgggcaatgg tatggagcca
     1381 cgcggcaagc gatccgagag ccgggttgaa gagctcggcc cggttccaga gggcgagaaa
     1441 cgtctcctgg accacctcct cggcaagctg ccgatccgac gtcaaccggc tggcggctgc
     1501 gaagaccgcg tcgacatacc ggtcgtacag ttcgcccagg gccaactgcg accccgccgc
     1561 gacttcgcga acgagggccg catcgtcaat cggcggacga tccccaacca tcgctacgtt
     1621 cccgacgcgt cgtcggtttc ggccgggatg tggaggggcc ggcgctcgaa cccactcgag
     1681 aacacgacat cggtctcggt cgagaagaca tcctgcgtcg actgcacgcg gcggaggacg
     1741 cgctcgaggt cgcgggtgtc gcgggcacgg accttgatga ggtaatcggc ctcgccggtg
     1801 atgtggtggc actcctcgat ctcggggatc gccgcaaacg aatcggtcag gtcgtgggcg
     1861 acggtgccgg gtgcctgggt gacccagctg aacgccagga tgtcgtagcc gagggtgcgc
     1921 ggatcgacga tcgcggcgta gcccgtgatg acgccgcgcg cctcgagctt cttgacccgt
     1981 tcgtgggccg atgggccgct catgccgacg tcgccgccga gatccgcgta gggccgccgg
     2041 ccgtcccgct ggaggacgtc gagaatgtgc gcgtcattcg tactgatgcg ttcagacaag
     2101 gccatctggt accaattcgt caggtggatc gattgtgttg tcgcgattcg ttaggtatga
     2161 tgacagagaa gaccactttg atcgtcggtc cgccgcactc cagcggcggg agctggttca
     2221 accgggctcc gcggcgatca cccagcggag gtaccccgat gttcgcaatt ggactcgggt
     2281 tcctggtgct gttctcgacc ctaagcatcc tgctcggcac cgaggattcg caccacagca
     2341 cggaccctcg ggacacgctc gcgatctggg cacgcgtcgg cgcacgctga tcccggctcg
     2401 atagcctacc ccacgaggtc ccgaacgccg tttcgggacc tcgtccggtt tctgcctcag
     2461 ggcgcgcgtc gggcgagatc cgccttccat gccgtttcga atagccgaat ggcggcccgc
     2521 gccggggtgg gtcgccggcc gttcgcattc ggctattcgc actggcgtgg ggggttcgcg
     2581 ggcgagcgcc acacgcacgg agccggcgcg agcgggcgcg agcggttcag gcctgggcct
     2641 gagcctgagc tcgcgcctgc ttgatcagct ggacaatcgc catggcgtgg ccgaggccgt
     2701 ggtcggtctt gagccagttg acgatcacgg tcgcctcatc gctgccccct gttcggtgac
     2761 gcacgctggc gatcgcgatc ctcaccattc tcctgcgggc cggcggacga ccgctggcag
     2821 acgccccgcc ggaaggtggg cctcgagaca ggtgacgccc ttcggtccga cggtcgcgtc
     2881 atgtcgcgtg ccggctggca gctccaaccg atcgccgacg tcgagcgcga cggagccgcc
     2941 gtcatccggc agcccgaatg cgatcgaccc gctggccacg acgatcacct tgtcgtatcc
     3001 gtgatcatgg gcgccgtacc gatcgcccgg tccgttcgac cacgacgacg ggtcgagccc
     3061 ttcggcccgg atccgggccg caacgtccat cacccggcga ccgaaacgcc gatgatcgtc
     3121 ccgacgacgt acgtcacgat cgcggcggcc gcgccgatcc cgacctggcg cgccccggag
     3181 aagaggacgc tccggcccgt cagcaggctg accccggccc cgacgccgaa caaggcgacg
     3241 aggctcaggc cgatcgcggc aacgaacgcg ccgaccccgc caccgacaag gtacgggacg
     3301 accgggacga atgccccgag ggcgaacgca atgaaggatc cgaacgcggc gccgaacggc
     3361 gagccgagct cgtcgggatc gaggccgagc tcctcgcgga tcagctgatc gagggcccgc
     3421 tcgggatcgt cgaagagacg atgggcgatc gccttcgcct cgtcggcggg gaagcccttc
     3481 gcccggtaga gggcggccat ctccgcctcc tcctcttcgg gcatcgcctc catctcggcc
     3541 cgctcgaggg cgatctggcg ctcgaagagc tcgcgctggc tctgcatcga gatgtactcg
     3601 ccggcggcca tgctgaaggc tccggcgagc aggccggcga cccccgcgag caggatgaaa
     3661 tggccggcgg tcgctgtcgg tccggacgcc ccggcaacgc ccatgacgag ggcgaggttc
     3721 gagacgaggc cgtcgctgac gccgaagatc acggcccgca gcgtcccgga ccggcccgac
     3781 cggtgccact tctcgcgggc ggcgatgtcc cggtcggggt ggtagtcggg gtccttgagc
     3841 ttgcgccaga tctcggcgtg ctcgcgctcg tc
//
LOCUS       DATAIW010000136        13454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211286, whole genome shotgun sequence.
ACCESSION   DATAIW010000136 DATAIW010000000
VERSION     DATAIW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211286"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1548)
                     /locus_tag="VHM48_04385"
     CDS             complement(91..1548)
                     /locus_tag="VHM48_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX2754674.1"
                     /translation="MIRRPLPVAIRPRGVILGVTAAVLVAGAAVGLSKSDPGQSYADL
                     LAAQSQAIYGFGHPLDHAATGTFTGPGDQAVELAKGLTARVVSSVIGENPDMIGLWPD
                     DSAPTDAIICNEITGNVAGAPATLQRVHLADGVVSDIAFGLVSCDPVHRTAWGTIVFG
                     EENGNLGRLYELLDPLTTSGVQIDRATGTTSDPVHVVARTALGQVSYEGLVSLPDGTT
                     YYADELRPSNGKPGGGVYKFVPSHRYAGSSPISNLADSPLAAGSVSVMRLGIRNGGTD
                     FGQGSNTGAGRWVPLGTSPGATWNLAAAALAAGGYTGYYRPEDMDLDPLAWADGRIRA
                     CWNNTGNDEAENWGETMCLEDVVDASVATGRHPVVEPFVIGNADLRMPDNLDFQPGTG
                     ILYILMDATTSAADPNRSNDSIWACLPDGTDSDILSDGCVRVMNLKDGAAEFTGIEFL
                     GDGKSFLVHLQHRDQTGRAVPGTSDEILVSGLATH"
     gene            1724..1894
                     /locus_tag="VHM48_04390"
     CDS             1724..1894
                     /locus_tag="VHM48_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754675.1"
                     /translation="MTGSRSRTARLALGLAVFVIGFGGAAVLDNAALALAGSLGLIAA
                     RLTVERYPRSRD"
     gene            complement(2213..4243)
                     /locus_tag="VHM48_04395"
     CDS             complement(2213..4243)
                     /locus_tag="VHM48_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX2754676.1"
                     /translation="MSPTLRNGKPIEIELKYRVVDAAAGDRYLAADVIAGFRPSSPVR
                     STQVEDRYIDTAGGAMARAGFAARLRQTAKTTTVAVKSSVRRVGTGNVHRREELEGPA
                     DRTAHPRDWPPSDARSLILEQCGDAPLVEVVTIRQLRRKRILELDGTSVELSLDEVDA
                     VSRSRVVGRFVELEVELMSGDEAGLAAIDRELADDPGLAPADTSKLQSALRAVAAAEP
                     KRGRRGGAVLPDIEDDRPRDRDADAPVPPIEEIVEAVLAELDATTTVADEVDEVEAAD
                     PDAFGATDARDVETGPATPADAARSEAAAAATAEPSAEPGAERTPDGRRSSNDDRIKA
                     SEPVKVRSKAKAAAATTDEGGAKDEDVRPHLVVGKTPGVLADDHVAEAGRKVLRFHLA
                     KMIAKEAGTRDGVDPEDLHGMRVATRRQRAAWRVFGEAFRPERTKKHRGRLREVAARL
                     GAVRDLDVLIEAADAYRADLAVAEQRALEPLLAAWRLHRDDARQLLMRELDSDGYRRW
                     LDDYAEFVRHEGVAARPVVPTEPHRVRDTAASRIHSAYEHVRSYEPVLRWADVDTLHE
                     LRIHGKWLRYSLEFVREALGPDADGLIARVTALQDHLGLMKDADVAAHLARSFLVERA
                     GSISDGESAAIARYLVSREKEVARLHRTVGRSWRGVGGIAFRRALGRALASL"
     gene            complement(4266..6494)
                     /gene="ppk1"
                     /locus_tag="VHM48_04400"
     CDS             complement(4266..6494)
                     /gene="ppk1"
                     /locus_tag="VHM48_04400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX2754677.1"
                     /translation="MSASAGASGHVNGPVKGRTARASARAAGGHSRSSRAPRPDRSAA
                     RPYINRELSWLEFNARVLFEARDERNPLIERVRFLTIFAGNLDEFFQVRIAGLRQQVA
                     TGSGSKSPDGRTAAEQIAASGARIRELVTEHSTIWVDIRRALAATGIEIVRYNAIPEH
                     HAMLRKRFIDEIYPVLTPLAVDPGHPFPYISTLSLSIAVGLRDPETGESHFARVKVPQ
                     ILPRLYEVEPHRFVLLDQIIEDNLDLLFSGMDIVEHHLFRVTRNADLSIEEDEADDLL
                     MAIEEELRRRRFGEAVRLEVERSMPAGTREILLRGLGLGADDAYEVRGMLDLTGLRDI
                     LELDRPDLKSPPWTPVTPPRLVPPDDDEPADVLAAIRAGDVLVHHPYDSFVASVERFL
                     GQAVDDPEVLTIKLTLYRTSGDSPIVRDLIRAAEQGKQVVVLVEIKARFDEEANIVWA
                     RKLEQAGAHVVYGLVGLKTHSKVCLVVRREGSGLRRYVHIGTGNYNQRTARLYTDLGL
                     LSCRPELGADVTDLFNVLTGLSRQRSFRRLLVAPHSLRSRFLELVDREIASAAVGHEA
                     RIVVKLNALVDQPSIDALYVASQKGVRVDVIARAACSLLPGVKNISTGIAVRSIVGEF
                     LEHSRIWGFANGGEGEWYIGSADLMDRNLDRRVEAMVPVEDAEARNRIAEIIDIMLQD
                     DRRSWQLGPNGEWRRTEEIQGHPGICDTHEELKARAIASTVSAATPHRPRAGVGSLDP
                     RA"
     gene            complement(6667..7017)
                     /locus_tag="VHM48_04405"
     CDS             complement(6667..7017)
                     /locus_tag="VHM48_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754678.1"
                     /translation="MLGATTRLAVAAVATLFLVGGLALISVGDGATGTGLWLVVIGAG
                     ALIALAIERNRYRSEESERSFESAGPGGGEPSGRLDPRFRRTEETFIDPTTGVTMRVF
                     VDRGTGERRYVGER"
     gene            complement(7028..7372)
                     /locus_tag="VHM48_04410"
     CDS             complement(7028..7372)
                     /locus_tag="VHM48_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX2754679.1"
                     /translation="MIVATTPFIAGHRIVETKGQVFGLVVRSRGFSGNLIAGLRSLGG
                     GEIKEYTSLLEDTRRQALDRLVKNATLMGGNAVVSMRFDSSEMATTMTEIVAYGTAVV
                     IEADASAPQATE"
     gene            complement(7369..7869)
                     /locus_tag="VHM48_04415"
     CDS             complement(7369..7869)
                     /locus_tag="VHM48_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX2754680.1"
                     /translation="MTQERDSIDSSGAGGSAGPATAVATVATGPSGSPTAPLTIHADR
                     VAATLAIGWADGHATLYDFVALRWLCPCAYCRGEAGMPGWLDSAPTLTAEQTRMIDIH
                     LVGNYAVSPHWGDGHSTGFYTFNLLRDRCPCPECTARRGQAGHQHEDGHGARPEAATS
                     TTEGVP"
     gene            7941..8921
                     /locus_tag="VHM48_04420"
     CDS             7941..8921
                     /locus_tag="VHM48_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2754681.1"
                     /translation="MSLLIDDLRKSFGRVVALDGLSFEVPSGQVFGFLGANGAGKTTT
                     MRIALGVLAANSGRVVWDGTDTRELPRATWGYLPEERGLYPRMVVLDQLVFFASLHGV
                     PRDIARRDALAWLHRLRVTDLAERKAEELSKGNQQKIQLISAILHNPPVLLMDEPFTG
                     LDPVNVALLREAFLELRDDGRTLIFSTHQMETVEAMCESIAIVDHGRVVVGGPLRQIK
                     RASGRRLVRLSIDGDHRMAWLAAVPGARILRPGIDRTEIELDEGTEPDAVLTAAIAAG
                     ARVSHFEVGEPSLEQIFIDHVGRPADEESHLASDPSGAAVAPAATAEDAA"
     gene            8918..10231
                     /locus_tag="VHM48_04425"
     CDS             8918..10231
                     /locus_tag="VHM48_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754682.1"
                     /translation="MSGLPSAPGRHEPAFPNTTHVARREYLELVRSRLFHVSTVTLAV
                     LAILVALLPIVARLAERGTVTQVAIVSSDADLSERTIGTLDLLLNQNGTSRSGGYKLT
                     ALTNETDAIAGIDARRYDAYLVAARSPDGHLSFQFHLGETAGDAVVQQLSIGVFGVAV
                     LDFSDRNSASGFRLPQLDVLRQSGEGGGAAPYSASNSASRLVVGLVFGILIFITIVIY
                     GMWVAAGVVAEKSSRVMELLVSAASPRQLVIGKALGIGLAGGTQYLLVLAPAIATLLL
                     EDRIASAVLGAGAGIALSLEALSPTLLIAFFVFYTLGFALYALVYAAAGSLVSRPEDL
                     QIIALPLSLIAIVGYLQAILALTGGITGFIRFASYVPFWSPFVMLTRLSVGRVEPIEL
                     LISIALLLATIVVTGIVAIRIYSAGVLLYGQRPGLRAYVAAARHG"
     gene            complement(10372..11133)
                     /locus_tag="VHM48_04430"
     CDS             complement(10372..11133)
                     /locus_tag="VHM48_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754683.1"
                     /translation="MRDQLSLRLEPEIATLPNLPDGLRPMLPRPASEPFDSAAHLFEP
                     AWGGLRALAFVGPSEVAGGGLVRIVDGDGRDVGTMLPELGGMAVRLDARSAILDGELV
                     AVDDAGRADNEALERRIAGETGRAVAFLAFDLLHLDGRSLLTTPLHRRRELLKKVLHP
                     GDEVVAVPAIAAEGRALHAAAAAQGIGGIMARQRTSPYLPGVTSRLWQFVPVAPAVAA
                     GARAAQATLALEDRAPTAAAPVLALISRLPLLFDE"
     gene            11278..13449
                     /locus_tag="VHM48_04435"
     CDS             11278..13449
                     /locus_tag="VHM48_04435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX2754684.1"
                     /translation="MTRSFRPDDLYRFRIATDPRLSPDGSLAVFTVQTVAPAKDGYRH
                     ALWAVATDGDGSPRQLTIGVRHDRHGRFAPDGRSLAFLSDRRLHVEEEPAAGDAKQRE
                     DGQQVHLLPLDGGEARRLTDLPRGVDAFWWSPDGRQLLVTSSSRGATRDEDRRRRGKA
                     GQPKPGEAPESDYHFLDRLGYQANGAGLTHHVIDQLWIVEVATGDARRLNDEPAGVGD
                     AAWSPDGRRIVYTTNLRRDYDLEPRSHLVVVDADGGGRVRLTGDAATFAAPVWMPDGA
                     SIAAIGGYLPENVYGSDLWLIAADGSEATTRHGGRNLTGDHDVMLATTIGSDIVPSEG
                     HRAIPSADGHWLTFRAPIDGSVEIWRVATRDGSLERLTTGHHAIGAFDQVDLGGAGSA
                     VRTAWLCSSPTALNDLWLRDGATDAPRRSTDLNHAALAEIELREQVERWSIVDGRRIQ
                     GWFLAGVEGAGSSVGVVGPGGTLGRVGPGPLVTEIHGGPHTLYGWAPILEFQILAATG
                     MSVFFCNPRGSEGYGRDFNEANIRDWGPGPMRDVLAGVDALVAEGLANPARLGVTGGS
                     YGGYLTNWIVGHDDRFAAAMTCRSVSDMALLMLTGDISTGFWPFHEFRTTPWDDPAYF
                     REISPITYADRIRTPLLIQHSENDIRTTVGQAEALFTVLRSKKRPVRLLRVPEETHEL
                     TRSGAPFRRVENLAVVRDWFRHFLVDGKRRLPPLPKIHGGR"
BASE COUNT         1862 a         4786 c         4798 g         2008 t
ORIGIN      
        1 cgggtggatc gcctgaggtt cgcacgcacc ggctgagtcg aacgagcccg gtcgggtcag
       61 ccgaccgggc tcggacggtg cttgggctgc tcagtgggtg gcgagccccg agacgaggat
      121 ttcgtcgctc gtccccggga cggcgcgccc ggtctgatcg cgatgctgga ggtggacgag
      181 gaagctcttc ccgtcgccga ggaactcgat cccggtgaac tcggcggctc cgtccttgag
      241 gttcatgacc cggacgcagc cgtccgacag gatgtcgctg tcggtgccgt caggcaggca
      301 ggcccagatc gagtcgttcg accggttcgg gtcggctgcc gacgtcgtcg cgtccatgag
      361 gatgtacagg atcccggtcc cgggctggaa gtcgaggttg tccggcatcc ggaggtcggc
      421 attgccgatc acgaagggct cgacgaccgg gtggcggccg gtggccacgc tcgcgtcgac
      481 gacgtcctcg aggcacatgg tctcgcccca gttctcggct tcatcgttgc cggtgttgtt
      541 ccagcaggcc cggatccggc cgtcggccca ggcgagcgga tcgaggtcca tgtcctcggg
      601 ccggtagtag ccggtgtagc cgccggcagc cagcgcggca gcggcgagat tccacgtcgc
      661 gccggggctc gtgccgagcg gcacccatct gccggcgccg gtgttcgagc cctggccgaa
      721 gtcggtcccg ccgttccgga tgccgaggcg catgacggac acgctccccg ccgcgagcgg
      781 actgtccgcg aggttcgaga tcggcgagct gccagcgtac cgatggctcg ggacgaactt
      841 gtagaccccg ccgcccggct tgccgttgga cgggcgcagc tcatccgcgt agtaggtggt
      901 gccgtcgggc aggctgacca ggccttcgta gctgacctgg ccgagcgcgg tccgggcgac
      961 gacatgcacc gggtcgctcg tcgtgcccgt cgcccggtcg atctggacgc cgctcgtcgt
     1021 gagcgggtcg agaagctcgt acagtcgccc gaggttccca ttctcctcac cgaacacgat
     1081 ggtgccccac gcggtgcgat gcaccgggtc gcaggagacg aggccgaagg cgatgtccga
     1141 gacgacaccg tcggcgaggt ggacgcgctg gagcgtggcc ggggcgcctg ccacattccc
     1201 cgtgatctcg ttgcagatga tcgcgtcggt cggcgcgctg tcgtccggcc acaggccgat
     1261 catgtcgggg ttctcgccga tcaccgacga cacgacgcgg gccgtgaggc ccttcgcgag
     1321 ctcgacggcc tgatcgccgg ggccggtgaa cgtgccggtc gcggcgtggt cgagcgggtg
     1381 accgaagccg tagatcgcct ggctctgggc ggccaacagg tcggcgtagc tctggcctgg
     1441 atcggacttc gagagaccga ctgcggcccc ggcaacgagg acggcggccg tgacacccag
     1501 gatcacgcca cgcgggcgga tggcgacggg caacgggcgg cggatcatcg atctgctcct
     1561 ccgagctgga cgattggaca tccccagcgt cgggcgagcg cgtcaacgcc cggtatgcgt
     1621 caggtcaacg atcgaccgac gccgtcgatc gagattcggt cagcggcccg ctcggagcgc
     1681 ccgcgttcac gagccggtaa cacgggccga cgagactgcg cccatgaccg gatcgagatc
     1741 tcgaacggcc cgcctggcgc tcggcctggc ggtcttcgtg atcggcttcg gtggtgcggc
     1801 cgtcctcgac aacgccgcgc tcgccctcgc gggaagcctc ggcctcattg ccgcccggct
     1861 caccgtcgag cgctatcccc gcagtcgcga ctgacgggcg ccgacgagac cgaaggtcag
     1921 gcgcgagccg gcgccgccag cttcgggcgg tcgccggcga cgctgagcca ggcggcccgg
     1981 atcgacgggc gcggccgcga cggccggcgg gtcgtcgtgc tgagacgaca ggccgcgaaa
     2041 cgccagccgc gtccatcggt gcgcgtgatc gggcgggagg agcggtcggg ctggagtcgt
     2101 gcgttcatgc cgcatgtctg gatggccgca tgcgacgggc cgatgcctgg ccggccgcgg
     2161 tcgttgcagc gtcgttgcag agccgccgcc ggacgcgccg ccggacgcat cctcagaggc
     2221 tggccagcgc ccgcccgagc gcgcgtcgaa aggcgatccc gccaaccccg cgccaggagc
     2281 gaccgacggt ccggtggagg cgggcgacct ccttctcgcg actcacgaga tagcgggcga
     2341 tcgccgcgct ctcgccgtcg ctgatcgagc ccgcacgctc gacgaggaag ctccgggcga
     2401 ggtgggcggc cacgtcggcg tccttcatca ggccgaggtg atcctgaagg gcggtgactc
     2461 gggcgatgag gccgtcggcg tccgggccga gcgcctcccg gacgaattcc agcgagtagc
     2521 gcaaccactt gccgtggatc cgcagctcgt ggagggtgtc gacgtcggcc cagcggagga
     2581 ccggctcgta ggagcggaca tgctcgtaag cggaatggat ccgcgacgcg gccgtgtcgc
     2641 ggacgcgatg gggctcggtc gggacgaccg gtcgcgcggc gacgccctcg tggcggacga
     2701 actcggcata gtcgtcgagc cagcgccgat agccgtccga atcgagctcc cgcatcagca
     2761 gctgccgggc gtcgtcacga tggagccgcc aggcggccag gagcggctcg agggcgcgct
     2821 gctcggcgac cgccagatcg gcccgatacg catcggccgc ctcgatcagg acgtcgaggt
     2881 cgcggacggc cccgaggcgg gcggcgacct cccggagccg gccgcggtgc ttcttcgtcc
     2941 gctccggccg gaacgcttcg ccgaacaccc gccacgcggc tcgctggcga cgggtggcga
     3001 cgcgcatccc gtggagatcc tcgggatcga caccgtcgcg ggtgccggcc tccttggcga
     3061 tcatcttcgc caggtgaaag cggaggacct tccggcccgc ctcggcgacg tgatcgtcgg
     3121 cgaggacgcc cggggtcttg ccgacgacga ggtgcggtcg aacgtcctcg tccttcgccc
     3181 cgccctcatc ggtcgtagcc gccgcggcct tcgcctttga tcgcaccttg accggttcgg
     3241 acgccttgat ccgatcgtcg ttcgacgacc tgcgcccgtc cggcgtccgc tcggcaccgg
     3301 gttcggcact gggttcggca gtggctgcgg cggctgcctc cgaccgtgct gcgtcggccg
     3361 gcgtggcagg gccggtctcg acgtcgcggg cgtccgtcgc accgaaagca tccggatccg
     3421 ccgcctcgac ttcgtcgacc tcgtccgcaa cggtcgtcgt cgcatcgagc tcggcgagca
     3481 cggcctcgac gatctcctcg attggcggca cgggcgcatc tgcgtcccgg tcccggggcc
     3541 ggtcatcctc gatgtcgggc agcacggccc cgccgcggcg accacgcttc ggctccgcgg
     3601 cggcgaccgc ccgaagcgct gactggagct tggacgtgtc ggccggggcc aggcccggat
     3661 cgtcggcaag ctcgcggtcg atggcggcga ggccggcctc gtcgccgctc atcagctcga
     3721 cttcgagctc gacgaaccga ccgaccaccc gactccggct gaccgcatcg acctcgtcga
     3781 ggctgagctc gaccgacgtg ccgtcgagct cgaggatccg cttgcgacgg agctggcgga
     3841 tcgtgacgac ctcgacgagc ggcgcatcgc cgcactgctc gaggatcagc gagcgagcgt
     3901 cggacggcgg ccagtcgcgc gggtgggcgg tccgatcggc cgggccctcg agctcctcac
     3961 ggcgatggac attgccggtc ccgacgcgcc ggacggacga cttcacggcg accgtcgtcg
     4021 tcttggccgt ctggcgcagg cgggcggcga acccggcccg ggccatcgcc ccgccggcgg
     4081 tgtcgatgta gcgatcctcg acctgggtcg accggaccgg tgagctcggt cggaacccgg
     4141 cgatcacgtc cgcggcgagg tagcgatcgc ccgccgccgc gtcgaccacg cggtacttca
     4201 gctcgatctc gatcggcttg ccgttgcgta gggtcgggct catcgtcgcc tctggtctgg
     4261 gtcggtcatg cgcgcggatc cagcgaaccg acaccggccc gcggtcggtg cggggtcgcc
     4321 gcgctcacgg tcgaggcgat cgcccgggcc ttgagctcct cgtgcgtatc gcagatgccg
     4381 ggatgaccct ggatctcctc ggtccgccgc cactcgccat tcggcccgag ctgccacgac
     4441 cggcggtcgt cctggagcat gatgtcgatg atctccgcga tccggttgcg cgcctcggcg
     4501 tcctcgaccg ggaccatcgc ctcgacccgt cgatcgaggt tgcgatccat caggtcggcc
     4561 gagccgatgt accactcgcc ctcgccgccg ttggcgaagc cccagatccg cgagtgctcg
     4621 aggaactcgc cgacgatcga acgcaccgca atgccggtcg agatgttctt gacccccggc
     4681 aggagcgaac aggcggcccg ggcgatgaca tcgacccgga cgcccttctg cgacgcgacg
     4741 tagagggcat cgatcgaggg ctgatcgacc agggcgttga gcttgacgac gatccgcgcc
     4801 tcgtggccga cggcagcact ggcgatctca cggtcgacca gctcgaggaa ccgtgaccga
     4861 aggctgtgcg gggcgaccag cagccggcgg aacgaccgct ggcgcgacag gccggtcagc
     4921 acgttgaaca gatcggtcac gtcggccccg agctccggcc ggcagctcag caggccgagg
     4981 tcggtgtaga gccgggccgt ccgctggttg tagttgcccg ttccgatgtg gacgtagcgg
     5041 cgcagcccgg aaccctcgcg ccggacgacg aggcagacct tcgaatgggt cttgagcccg
     5101 acgaggccgt agaccacgtg ggcgccggcc tgctccagct tgcgggccca gacgatgttc
     5161 gcctcctcgt cgaagcgggc cttgatctcg acgaggacga cgacctgctt gccctgctct
     5221 gccgcccgga tcaggtcgcg cacgatcggc gagtcgcccg atgtccggta gagcgtcagc
     5281 ttgatcgtca ggacctcggg atcgtcgacg gcctggccga ggaaccgctc gaccgaggcg
     5341 acgaagctgt cgtaggggtg gtgcacgagg acgtcgccgg cccggatcgc ggcgagcacg
     5401 tcggcgggct cgtcgtcgtc gggcgggacc agccgcggcg gggtgaccgg cgtccatggc
     5461 gggctcttga ggtccggccg atcgagctcg aggatgtcgc gcagaccggt caggtcgagc
     5521 atgccccgga cttcgtaggc gtcgtcggcg ccgaggccga gaccgcgcag gaggatctcc
     5581 ctggtgccgg ccggcatcga gcgctcgacc tcgaggcgga cggcctcgcc gaagcgacgc
     5641 cggcggagct cctcctcgat cgccatgagc agatcgtccg cctcgtcctc ctcgatcgag
     5701 agatcggcgt tgcgggtcac cctgaagagg tggtgctcga cgatgtccat gccgctgaac
     5761 aggagatcga ggttgtcctc gatgatctgg tcgagcagga cgaagcggtg cggctcgacc
     5821 tcgtacagcc ggggcaggat ctggggcacc ttgacccggg cgaagtggct ctccccggtc
     5881 tccggatcgc gcagccccac cgcgatcgag aggctgagcg tcgagatgta cgggaacggg
     5941 tggccgggat cgaccgcgag cggggtgagg accgggtaga tctcgtcgat gaaccgcttg
     6001 cggagcatcg cgtgatgctc cgggatggcg ttgtacctca cgatctcgat gccggtcgcg
     6061 gcgagggccc gccggatgtc cacccagatc gtgctgtgct ccgtgacgag ctcccggatc
     6121 cgcgcgccgg aggcggcgat ctgctccgcg gcggtccgac catccggtga tttcgagccc
     6181 gagccggtcg cgacctgctg gcgaagaccg gcgatccgga cctggaagaa ctcgtcgagg
     6241 ttgcccgcga agatcgtcag gaaccggacg cgctcgatca gcgggttgcg ctcatcgcgg
     6301 gcctcgaaca agacgcgggc gttgaactcc agccaggaca gctcgcggtt gatgtacggg
     6361 cgtgctgccg agcggtcggg acggggcgcc ctggagctgc gcgagtggcc accggcggca
     6421 cgcgccgacg cgcgcgcggt gcggcccttg actggcccgt tgacatgccc gctggctccg
     6481 gcggaggcgc tcatcgcggt ggtgttcggc tgcctcacag tggccgggcg cgatccagcg
     6541 ctcgggccgg gcaggatatc acgggacctc gggcgaatgg gtctcgaggc ctcgccgaag
     6601 ctcggccgga tgagctccgc cttcgacggc ggtgagcgaa cgcctcagca gcgctgaggt
     6661 tgccggctac cgttccccga cgtagcgacg ttcgccggtc cctcgatcga cgaagactcg
     6721 catcgtgacg cctgtcgtcg ggtcgatgaa ggtctcctcg gttcgccgga accggggatc
     6781 cagacgaccg gacggctcgc cgccgcccgg cccggccgat tcgaacgatc gctcggattc
     6841 ctcggagcgg tagcggttgc gctcgatcgc cagggcgatc agggcgcccg cgccgatcac
     6901 gacgagccac aggccggtcc cggtcgcccc atcgccgacg gagatcaggg ccaggccgcc
     6961 gacgaggaac aacgtcgcga cggcggcgac ggccagtcgc gtcgtggcgc cgagcatcgc
     7021 gctgcggtta ctcggtcgcc tggggcgccg aggcgtcggc ctcgatcacg acagccgtcc
     7081 cgtaggccac gatctcggtc atcgtcgtgg ccatctcgga gctgtcgaag cgcatcgaga
     7141 cgacggcgtt gccgcccatc agggtcgcgt tcttgacgag ccggtcgagc gcctggcgac
     7201 gggtgtcctc gagcaacgac gtgtattcct tgatctcgcc gccgccaagc gagcggaggc
     7261 cggcgatgag gttgccggag aagccacggc tgcggacgac gaggccgaag acctggccct
     7321 tggtctcgac gatccggtga ccggcgatga atggggtggt ggcgacgatc atgggactcc
     7381 ctcggtcgtg ctggtggccg cctcgggccg ggccccgtgc ccgtcctcgt gctggtggcc
     7441 ggcctggccg cgacgcgccg tgcactccgg gcacggacag cgatcgcgga gaaggttgaa
     7501 cgtgtagaac ccggtcgaat ggccgtcgcc ccaatggggc gacaccgcgt agttgccgac
     7561 gagatgaatg tcgatcatcc gggtctgctc ggcggtcagg gtcggggcgc tgtcgagcca
     7621 gcccggcatc ccggcctcgc cgcggcagta ggcgcacggg cagagccagc gcagggcgac
     7681 gaagtcgtag agggtcgcgt ggccgtcggc ccaaccgatg gccagcgtcg ccgcgacgcg
     7741 atcggcgtgg atcgtgaggg gcgcggttgg cgagccgctg ggaccggtcg ccacggtcgc
     7801 gacggcggtg gcgggcccgg ccgagccccc ggcgccgctc gagtcgatgg aatcccgctc
     7861 ctgcgtcacg gggtcatcat cgcacctccc gggaccatcg tcccggcgcg acccggcccg
     7921 gcccgggcta tcctcggccg gtgagcctcc tcatcgacga tctccgcaag tcgttcgggc
     7981 gcgtcgtggc cctcgacggg ctgtcgttcg aggtcccgtc cggccaggtc ttcggcttcc
     8041 tcggggcgaa cggggccggc aagacgacca cgatgcggat tgccctcggc gttctcgcgg
     8101 cgaacagcgg tcgagtcgtc tgggacggca cggacacccg cgagctgccg cgcgcgacgt
     8161 ggggctacct gccggaggag cgcgggctct acccgcggat ggtcgtcctc gatcagctcg
     8221 tgttcttcgc ctcgctccac ggcgtccccc gcgacatcgc ccggcgcgat gccctcgcct
     8281 ggctccaccg cctgcgggtc accgacctcg ccgagcgcaa ggcggaggag ctcagcaagg
     8341 gcaaccagca gaagatccag ctgatctcgg cgatcctcca caatccgccg gtcctcctga
     8401 tggacgagcc cttcacgggt ctcgacccgg tcaacgtggc gctcctccgc gaggcgttcc
     8461 tcgagctccg tgacgacggc cggacgctga tcttctcgac ccaccagatg gagaccgtcg
     8521 aggcgatgtg cgagtccatc gcgatcgtcg accacggcag ggttgtcgtg ggtgggccgc
     8581 ttcgccagat caagcgggcg agcggccggc gattggtccg gctctcgatc gacggcgatc
     8641 accggatggc ctggctggcc gcggtgcccg gcgcgcggat cctgcgaccc ggcatcgacc
     8701 ggacggagat cgagctcgac gaggggaccg agcccgatgc cgtcctcacg gcggcgatcg
     8761 cggccggtgc cagggtcagt cacttcgagg tcggcgagcc gtcgctcgag cagatcttca
     8821 tcgaccatgt cgggcggccg gccgacgagg agtcgcacct ggcgtccgat ccgtccggcg
     8881 ctgccgtcgc gcccgccgcg accgcggagg atgcggcgtg agcgggctgc cgtccgcgcc
     8941 gggccgccac gaaccggcct tcccgaacac gacccacgtc gcccggcgcg aatacctcga
     9001 gctcgtccga tcacggctct tccacgtctc gacggtgacc ctcgccgtgc tcgcgatcct
     9061 ggtcgccctg ctccccatcg tggcccggct tgccgagcgt ggcacggtca cccaggtcgc
     9121 catcgtctcg agcgatgcgg acctgtccga gcggacgatc gggacgctcg acctgcttct
     9181 caaccagaac ggcacgagca ggtcgggtgg ctacaagctc accgccctga cgaacgagac
     9241 cgacgcgatc gcgggcatcg acgcgcgccg ctacgacgcc tatctcgtcg cggcccgctc
     9301 gcccgatggg cacctgtcct tccagttcca tctcggggag acggccggcg acgccgtcgt
     9361 ccagcaactc tcgatcgggg tgttcggtgt ggcggtcctc gacttctcgg atcgcaactc
     9421 agcgagtgga ttccgcctgc cgcagctcga cgtcctgcgc cagtcgggtg agggcggcgg
     9481 cgcggccccg tacagtgcgt cgaactcggc cagccggctg gtcgtcggcc tcgtgttcgg
     9541 gatcctcatc ttcatcacga tcgtgatcta cggcatgtgg gtcgcagccg gggtggtcgc
     9601 cgagaagtcg agtcgcgtca tggagctcct cgtcagcgcc gcctcgccgc gtcagctggt
     9661 catcggcaag gccctcggga tcgggctggc cggggggacg cagtacctgc tcgtcctcgc
     9721 gccggcgatt gccacgttgc ttctcgagga tcggatcgcg tcggccgtgc tcggcgccgg
     9781 cgccgggatc gccctctccc tcgaggctct gtcaccgacg ctcctgatcg cgttcttcgt
     9841 cttctacacg ctcggatttg ccctctacgc gctggtctat gcggccgccg ggtcgctcgt
     9901 cagccggccc gaggatctcc agatcatcgc cctgccactg agcctgatcg cgatcgtcgg
     9961 ctacctccag gcgatcctcg ccctgaccgg cggcatcacc ggcttcatcc ggttcgcctc
    10021 gtatgtgccg ttctggagcc cgttcgtgat gctgacgcgg ctgtccgtcg gccgggtcga
    10081 gccgatcgag ctgctcatct cgatcgcgct gctgctcgcg accatcgtcg tcaccggcat
    10141 cgtcgcgatc cggatctact cggcgggcgt cctgctctac ggccagcgcc cggggctgcg
    10201 cgcctatgtc gcggcggcgc gccacggctg aggtgagcgt cgagcctggc cggcgcaacg
    10261 ccgcctgaac gtgacccccg ctccgctttc gaatagccga atactgcgaa gttcgaatca
    10321 ttcggctatc cgcatcggat gggcgggtct gttcagacgc cgatcggcgc tctactcgtc
    10381 gaagagcagc ggcaggcggc tgatcagggc gaggaccggg gcagcggcgg tcggggcacg
    10441 gtcctcgagg gccagcgtcg cctgcgccgc acgggcgccc gccgcgacgg ccggcgcgac
    10501 cggcacgaac tgccagagtc gcgaggtcac gcccggcagg taggggctgg tccgctggcg
    10561 ggccatgatc ccgccgatgc cctgggccgc ggcggcggca tggagtgccc ggccctctgc
    10621 cgcgatcgcg ggcaccgcca cgacctcgtc acccgggtgg agcaccttct tcagcagctc
    10681 gcggcgacgg tggagcggcg tcgtgaggag cgaccgacca tcgagatgga gcaggtcgaa
    10741 ggcaaggaac gccaccgccc gacccgtctc gcctgcgatc cggcgctcca acgcctcgtt
    10801 gtcggcgcgg ccggcgtcgt cgaccgccac gagctcgccg tcgaggatcg ccgagcgggc
    10861 atcgaggcga acggccatcc cgccgagctc ggggagcatc gtcccgacgt cccgtccgtc
    10921 gccatcgacg atccggacga gcccgccgcc tgccacctcg gaagggccga cgaacgccag
    10981 cgcccgcagg ccgccccacg ccggctcgaa caggtgggcc gccgaatcga acggctcgct
    11041 cgccggtcgg gggagcatcg ggcgcaggcc gtccggcagg ttcggaagcg tcgcgatctc
    11101 gggctcgagc cgcagcgaca gctgatcgcg catcgtcggc cgatggtagc gctagactcg
    11161 acggcgtcgt cgacgatgcg tcgcgatcgc gcgaatcccg cggcacccga cccgtgccgc
    11221 cccgcgccgc gacgctcgcc catcacgacc ccaggacgtg cccggaggct gcccctcgtg
    11281 acccgctcgt tccgccccga cgacctgtac cggttccgga tcgcgacgga tccgcgcctg
    11341 tccccggacg gctccctcgc cgtcttcacc gtccagaccg tcgcgccggc caaggatggc
    11401 tatcgccacg cgctctgggc ggttgccacg gacggtgacg gctcgccccg ccagctgacg
    11461 atcggggttc gccacgatcg ccacggccgg ttcgcccccg acgggcggtc gctcgccttc
    11521 ctgtcggatc ggcggctgca tgtcgaggag gagccggccg ccggcgacgc gaagcagcgc
    11581 gaggacggcc agcaggtcca cctcctgccg ctcgacggtg gcgaggcgcg tcgcctgacc
    11641 gatctccccc gcggagtcga tgcgttctgg tggtcgcccg acggccggca gttgctcgtc
    11701 acgagctcgt cccgcggcgc aacccgcgac gaggaccgtc gccgacgggg caaggccgga
    11761 cagccgaagc ccggcgaggc gccggaatcc gactaccact tcctcgaccg gctcggctac
    11821 caggcgaacg gcgccggcct cacccaccac gtgatcgacc agctctggat cgtcgaggtg
    11881 gcgacgggcg acgcgcggcg cctgaacgac gagccggcgg gggtcgggga cgcggcgtgg
    11941 tcgcccgacg gccggcggat cgtctacacc acgaacctcc gccgcgacta cgacctcgag
    12001 ccccgatcgc acctggtcgt cgtcgacgcc gacggcggcg ggcgcgtccg gctgaccggc
    12061 gacgcggcga ccttcgcggc cccggtgtgg atgcccgatg gcgcgtccat cgcggccatc
    12121 ggcggctacc tgccggagaa cgtctatggc tccgacctgt ggctgatcgc ggccgatggc
    12181 tccgaggcga cgacccggca tggcggccgg aacctgaccg gcgatcacga cgtgatgctc
    12241 gcgacgacga tcggcagcga catcgttccc agcgagggtc accgcgcgat cccgtcggcc
    12301 gacggacact ggctgacgtt ccgggcgccg atcgacggca gcgtcgagat ctggcgcgtt
    12361 gcgacccgtg acggctccct cgagcgcctg acgaccggcc atcacgcgat cggcgccttc
    12421 gaccaggtcg acctcggcgg ggccggctcc gccgtccgca cggcctggct gtgctcgtcg
    12481 ccgaccgcgc tcaacgacct ctggctgcgc gacggtgcga cggacgcccc gcgccgcagc
    12541 acggacctca accacgccgc gctcgccgag atcgagctgc gcgagcaagt cgagcgctgg
    12601 agcatcgtcg acggtcgccg gatccagggc tggttcctgg ccggcgtcga gggggcgggg
    12661 tcgtcggtcg gcgtggtcgg ccccggcggc acgctcggcc gcgtcggccc cggaccactc
    12721 gtcaccgaga tccacggcgg gccgcacacc ctgtacggtt gggcgcccat cctcgagttc
    12781 cagatcctcg ccgcaaccgg gatgagcgtc ttcttctgca acccccgcgg ctcggaaggc
    12841 tacggccgcg acttcaacga ggcgaacatc cgcgactggg gcccgggccc gatgcgcgac
    12901 gtcctcgccg gcgtcgacgc cctcgtcgcc gagggcctcg ccaacccggc ccggctcggc
    12961 gtcaccggcg gctcgtacgg tggctacctg acgaactgga tcgtcggcca cgacgaccgc
    13021 ttcgcggcgg cgatgacctg ccgctcggtc agcgacatgg cgctgctgat gctgaccggc
    13081 gacatcagca ccgggttctg gccgtttcac gagttccgaa cgacgccctg ggacgacccg
    13141 gcctacttcc gcgagatctc gccgatcacc tacgccgacc ggatccggac gccgctcctg
    13201 atccagcaca gcgagaacga catccggacg accgtcggcc aggccgaggc gctgttcacc
    13261 gtccttcgct cgaagaagcg gccggtccgg ctgctgcgcg tccccgagga gacccacgag
    13321 ctgacgcgtt ccggcgcgcc gttccgtcgg gtcgagaacc tcgccgtcgt ccgtgactgg
    13381 ttccgccact tcctcgtcga cggcaagcgc cgcctgccgc ccctgccgaa gatccacggc
    13441 ggccgctgag gccc
//
LOCUS       DATAIW010000137         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_66232, whole genome shotgun sequence.
ACCESSION   DATAIW010000137 DATAIW010000000
VERSION     DATAIW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66232"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..643)
                     /locus_tag="VHM48_04440"
     CDS             complement(308..643)
                     /locus_tag="VHM48_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754685.1"
                     /translation="MTMDELDGVIAAPDHHRVLFENDVVRVIETTVRAGDTTPVHTHL
                     APTVLYVTSGSHFLRRDPDGAVMVDTRADPAFTLPRVMYSPGTPRHTLENTGDDDLVV
                     VGVELKDRG"
     gene            complement(807..1121)
                     /locus_tag="VHM48_04445"
     CDS             complement(807..1121)
                     /locus_tag="VHM48_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754686.1"
                     /translation="MANYLLVFRGGSMPQTKEEQDAVMAAWTAWFGEIGDALVDGGNP
                     ASRTKVLTKDGSVSDGGSGSPTGYSIIKADDMDGALALAKGCPVFLGGATIDVVETFS
                     VM"
     gene            complement(1261..1629)
                     /locus_tag="VHM48_04450"
     CDS             complement(1261..1629)
                     /locus_tag="VHM48_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754687.1"
                     /translation="MLTDSHAFSGFSSNDIPAAHTFYATTLGLDVSEEKGMLTLRLAG
                     GGQVIVYPKSNHEPASFTVLNFPVADVAVAVDELAAAGVVFERYPGMSQDERGIMRDE
                     GAAIAWFKDPAGNILSVLER"
     gene            1891..>2993
                     /locus_tag="VHM48_04455"
     CDS             1891..>2993
                     /locus_tag="VHM48_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX2754688.1"
                     /translation="MIHRRASWKRPFTVAAGLALAASLVAGSPVAQPGAVSATGPAPV
                     AEWTFDEGAGSTAADSVGNLDGAITGGATWTTGANGSAHAIQFNGTDGLVTVANNATL
                     EPAGNFTLSLWVKGGATTENVQHTILQKGDYGCDVGGSYRFGEAWNGTPDVFNSPALV
                     DVGLTAAGFAYPGYFSYQRPAWDGNWHHLLLVVEDSADRTSAWIDGYKTTTIWNNPDT
                     VRFGSAGRLDAALHFGGPGADCPSWKPFNGALDDVQLYGSALTDAQIYAMSPTYDPTV
                     SVSLGYWTLPSTPIPPVINDREIFFDAVISPMPGVDGTTTWYLSKGGAAEEVIGTSAL
                     APGGNGQTILRRAAGTLEPGSYTIRAQWEGAEN"
BASE COUNT          476 a         1047 c         1011 g          459 t
ORIGIN      
        1 ggccgagtac gggacccgct atcgggtcgc ctgggaggac gggcacgaaa gcacgatccg
       61 ccccgcggcg ggcagcgccc acgtgatccc ggccccgaag tcgacgaagg tcaaggggcg
      121 gcgccgctag cgagctccac cagggagctg cggtcggatc gggctgtccg ccgggtgtct
      181 ccatgaccca cgagtccgac atcgtcgctg agcgggcgat cgaatgagcg gccgatcagg
      241 cccgccccga tccgacgcaa ggctgcgaca tgggctggga acagccgcaa ccctgagccg
      301 cgggtcttca gccgcggtcc ttcaactcca cgccgacgac gacgaggtcg tcgtcgccgg
      361 tgttctcgag cgtgtggcgc ggcgtgcccg gcgaatacat gacccgtggc agggtgaacg
      421 ccggatcggc ccgggtatcg accatcaccg cgccatccgg atcgcgccgg aggaagtgcg
      481 agccagaggt cacgtacagc acggtcggcg cgaggtgggt gtggaccggc gtcgtatcgc
      541 cggcccggac cgtcgtctcg atgaccctga cgacgtcgtt ctcgaacagc acccggtggt
      601 gatcgggcgc ggcgatcacg ccgtcgagct catccatcgt catcgcgtcc accttgctgt
      661 cgggccccgg cggttcgcag tctaggtggc gcagacggat cgcgactcgg cgttgcggac
      721 gcgaaacagc cgccgaaccc gagggtcggc ggccgtcacc ggtcggtccg ggctcgcgga
      781 cccggtccgg ggctggatgc ccggacttac atcacgctga acgtctcgac gacgtcgatc
      841 gtcgccccgc cgagaaacac cgggcagcct ttcgccagcg cgagcgcacc gtccatgtcg
      901 tcggccttga tgatcgagta gccggtcggt gagccggacc cgccatcgct gaccgatccg
      961 tccttcgtca ggaccttggt ccgcgaggcc ggattgccgc catcgacgag cgcgtcgccg
     1021 atctcgccga accacgccgt ccacgccgcc atgaccgcgt cctgctcttc cttcgtctgg
     1081 ggcatcgagc cgccacggaa caccagcaga tagttcgcca tcgaaatcca tcctccaccc
     1141 gttgagctcg gtcgacgtgg tcgccgactc ggcgcgatca cgcgccgtcc acatcaacga
     1201 cgaacatgac ccgcccgaat cgacacgccc ggccggtcgg agtggtaaga ccgcgatccg
     1261 tcagcgctcg agcaccgaga ggatgttgcc cgccgggtcc ttgaaccagg cgatcgccgc
     1321 gccctcgtct cgcatgattc cgcgttcgtc ctggctcatg ccggggtacc gctcgaacac
     1381 gacgcctgcc gccgcgagct cgtcgacggc tacggcgacg tcggcgaccg ggaagttcag
     1441 gaccgtgaac gacgcgggct cgtggttcga cttgggatag acgatgacct gcccgccgcc
     1501 ggcaagtcgg agggtgagca tccccttctc ctcgctcacg tcgagtccga gggtcgtcgc
     1561 gtagaaggtg tgggcggcgg ggatgtcatt cgacgagaag cccgaaaagg cgtggctgtc
     1621 cgtgagcatg gtggtcctcc cgtgcatgcg atcgcgcatg cgacgtgtcc gcatggcgcg
     1681 gctcctgtcc atggtgaccg ctcgggcgac gagaactcat cggtgcaccg tggaccgcga
     1741 gtcggtgcgg cgcagcaccg gcgcgccaga tggtggcgat tcggctctgg attcggcggc
     1801 cgcagtcttg tagggtccgg cgcgacgatc gaccgaaatc gctcgtcatg gaggggccgc
     1861 ccacccggcg gcggaggttg gggagatccg atgattcatc ggcgggcatc gtggaagcgt
     1921 ccgtttacgg tcgcggccgg gctggccctg gccgccagcc tggttgccgg atcgccggtc
     1981 gcccagcccg gcgccgtgtc cgcgacgggt ccggcgcccg tcgccgaatg gaccttcgat
     2041 gagggggcag ggtcgaccgc ggccgattcc gtcggcaatc tcgatggcgc gatcaccggt
     2101 ggcgccacct ggacgaccgg cgccaacggc agcgcccacg cgatccagtt caacgggacc
     2161 gacgggctgg tcaccgtcgc caacaacgcg acgctcgagc cggccggcaa cttcaccctg
     2221 tccctctggg tcaagggcgg cgcgaccacc gagaacgtcc agcacacgat cctccagaag
     2281 ggtgactacg gctgcgacgt cggtgggtcg tatcgcttcg gcgaagcctg gaacggcacc
     2341 ccggatgtgt tcaattcgcc ggccctcgtc gacgtcggcc tgacggcggc aggattcgcc
     2401 taccccggct acttctcgta tcagcggccg gcctgggatg gcaactggca tcacctcctg
     2461 ctcgtggtcg aggactccgc cgatcggacc tcggcctgga tcgacggcta caagacgacg
     2521 accatctgga acaaccccga cacggttcgc ttcggatcgg ccggccgcct cgacgctgcc
     2581 ctccatttcg gcggacccgg cgcggattgc ccgtcctgga agccgttcaa cggcgcgctc
     2641 gacgacgtcc agctgtacgg ctcggccctg accgatgccc agatctacgc gatgagcccg
     2701 acgtacgacc cgacggtgtc ggtctcgctc ggctactgga cccttccatc gaccccgatc
     2761 ccgcccgtga tcaacgatcg cgagatcttc ttcgacgcgg tcatcagccc gatgcccggc
     2821 gttgacggca cgacgacctg gtacctcagc aagggcggcg ccgccgagga ggtcatcgga
     2881 acgtccgccc tcgcgcccgg aggcaacggc cagacgatcc tccgccgggc ggccggaacg
     2941 ctcgagcccg ggagctacac gatccgtgcg caatgggaag gcgccgagaa ctg
//
LOCUS       DATAIW010000138         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211319, whole genome shotgun sequence.
ACCESSION   DATAIW010000138 DATAIW010000000
VERSION     DATAIW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211319"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..368)
                     /locus_tag="VHM48_04460"
                     /pseudo
     CDS             complement(231..368)
                     /locus_tag="VHM48_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
     gene            complement(477..860)
                     /locus_tag="VHM48_04465"
     CDS             complement(477..860)
                     /locus_tag="VHM48_04465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX2754689.1"
                     /translation="MVGIGRTPARGGPTRGDIYIVAFPEAGGAVILGPHPALIVSTDR
                     MNRAGGTLLACPMTSQMQHDPEVYLPPYLVPADARSTGLSRDGYVKVDQVHTLSAAAL
                     GSFMGRANPEVMTAVDDALRFVLDL"
     gene            complement(854..1141)
                     /locus_tag="VHM48_04470"
     CDS             complement(854..1141)
                     /locus_tag="VHM48_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754690.1"
                     /translation="MFREAIEPYVAADPGLPSVKLSISLPADLAALVRSSAAESGSTV
                     SATIAAALRRTLGDAEQARLDAALELDREENLAFAQAYAPMAAELLAKLTW"
     gene            complement(1346..1615)
                     /locus_tag="VHM48_04475"
     CDS             complement(1346..1615)
                     /locus_tag="VHM48_04475"
                     /inference="COORDINATES: protein motif:HMM:NF021452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HEX2754691.1"
                     /translation="MPNADRPTSPMRISLSPERRSILVEAIQRHFAAEFDEPLSDFRA
                     EQLLDFFVAELGPPVYNQGVRDASSYIQEKLIDIEGDVFERETPR"
     gene            1885..2079
                     /locus_tag="VHM48_04480"
     CDS             1885..2079
                     /locus_tag="VHM48_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754692.1"
                     /translation="MLRIVFGLPTYETFQAAKRGELALGGCVVWPGHPTNRCTACGED
                     VVLDSDDRDDRDDRDDPEQA"
     gene            2193..>3481
                     /locus_tag="VHM48_04485"
     CDS             2193..>3481
                     /locus_tag="VHM48_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein/DEAD/DEAH box
                     helicase"
                     /protein_id="HEX2754693.1"
                     /translation="MPSAHTDVAASDASGSRVRDPETDDRAGRAAELKVETCVRAALP
                     VADGYRVFANVAWTGRTTAHGQLTDGEADLVIAHPELGFLVVETKAGEIRRDATGRWY
                     AGSRMLEPSPFEQAKRSLHALLGKLAELPDRPTDFHPIAGHAVALPDVDLESAGARLR
                     LAGPDIVAGLVLDRDALPPDDPPVTRAAVDRALDLWAADAPNRRAPGTAGVHLLEEIL
                     ATPVELRSLLRSEISAGERTVVELTNHQIAALRRLSAIRRMQVVGAAGTGKTLLAAKK
                     AEMLAREGFETLLVCFNQPLARLLAELTADTTRKRGRLTVSTFHQLCEDLATEAGVLP
                     PKPEPVTQDWFDRTLPDALLAAIDVLGWRFHAIVIDEGQDFAPDWLEALQLLLEDSRE
                     DVLYVFHDPAQAIFRDDTVERLGLAMYALDENCRNPGP"
BASE COUNT          536 a         1234 c         1214 g          497 t
ORIGIN      
        1 gcctcgcgac ggtcaacgcc aagttcgaca ccgggttccc gccgaccgcg tcgacgtacg
       61 aggtcgtcga ggtctcggcc cattgacgac gccacgggct ccccaacgca caccgccagg
      121 aacagccggc gctgccggtg cgaatagccg cataccgggg gttgcccgga gctccgacac
      181 gcggctgtta ttcggctatt cgaactcgca ccgctggctc gtcccggcga ctacaggaac
      241 ggatcccggg cgtcgatccc attgaagcgg cggaagtcgc ggtcgcgggt gtagatcagc
      301 cggacgccgt gctgacgcat cagggccgcg acatgcgcat cggggacgtc gttcccgcgc
      361 gggcgatcgc cggcagtgct gcggaagacc gaccagaaac cgtcggcctc gccgggcgcc
      421 cggacatgcg gccggtcatc gtcggggctg tccggctcga ggcgatccca gggcgctcac
      481 aggtcgagga cgaaccgcag ggcgtcatcg acggcggtca tgacctcggg attggcccgt
      541 cccatgaacg acccaagtgc cgcggcggac aacgtgtgga cctggtccac cttcacgtag
      601 ccatcacgcg agagcccggt cgagcgtgca tctgcgggca cgagataggg cggcagatag
      661 acctccggat cgtgttgcat ctgcgacgtc atcggacagg caaggagcgt gccaccggcg
      721 cggttcatcc gatcggtcga gacgatcaac gccggatggg gtccgaggat gaccgcgccg
      781 ccagcctcgg gaaacgccac gatgtagatg tcgccgcgcg tcggtccgcc cctcgccggc
      841 gtccgaccga tccctaccac gtcagctttg ccaacagctc tgccgccatt ggcgcatagg
      901 cctgggcgaa ggcgaggttc tcctcgcgat cgagctcgag ggcggcgtcg aggcgtgcct
      961 gctcggcgtc gccgagcgtt cgccgcagtg cggccgcgat cgtcgcactg accgtcgagc
     1021 cgctctcggc ggcggacgaa cgaacgagcg cagcgaggtc ggccggcagg ctgatcgaca
     1081 gcttgacgga gggcaaacca ggatcggccg cgacgtacgg ctcgattgcc tcgcggaaca
     1141 tccggggcct ggtttgatcg ttactactca tggtacgacc gacggtagga ccgcaggtga
     1201 cattcgtcaa gtggcgcgac gatcggcggc cgcgacggat ggacccgtac cgacgcgtat
     1261 gcggctattc gaaacgctgg cgggcatctc ctcgggcgtg gccacagtgt cagggcgggc
     1321 cgcccaaagg acgagtgggc cgcgctcacc gtggggtctc gcgctcgaag acgtcaccct
     1381 cgatgtcgat cagcttttcc tggatgtagc tcgacgcgtc gcggacgccc tggttgtaga
     1441 ccggcggccc gagctcggcg acgaagaagt cgaggagttg ctcggcgcgg aagtcgctca
     1501 ggggctcgtc gaactcggcg gcgaagtggc gctggatcgc ctcgacgagg atcgagcggc
     1561 gctccggcga gaggctgatc cgcatcgggg acgtcgggcg gtcggcgttc ggcatggcgg
     1621 gattctggca ggcccggaac ccggctgcgg cccgttctcc gcgtgatcgc gttcggcggc
     1681 cgagtcagac gtgcgctggc atttcgagta gccgaatacg agttcgggag gtcgtgacag
     1741 gctaggtgac acggcccggg gcgaggctcg gcacagatcg acggtcgacc gggaccgccg
     1801 acggcagggc aggggagccc gatcgaccga gtgacacgcg cgcccgccag cgccacctgt
     1861 ccgcgatgcg gagccgcggc tccgatgctt cggatcgtct tcggcctgcc gacgtacgaa
     1921 acgttccagg ccgccaagcg cggcgagctc gcgctcggcg ggtgcgtcgt gtggcccggg
     1981 cacccgacca atcggtgtac ggcctgcggc gaggacgtcg tcctcgactc cgacgaccgc
     2041 gacgaccgcg acgaccgcga cgacccggag caggcgtgag tccagcacgg attgaccaac
     2101 tggcctatcg tgacggtctc gtcgccccgc gtggcgcggc gccgtctcgg tcgcgacgcc
     2161 catggcggcc gaagactggg ggtctggacg tcgtgccgag cgcgcacacc gacgtcgccg
     2221 cttcggatgc ttctggttcg cgtgtccgcg accccgagac cgacgatcgg gccggtcgag
     2281 cggcagagct gaaggtcgag acctgcgtcc gggccgccct gccggtcgcc gacggatacc
     2341 gcgtcttcgc caacgtcgcc tggaccgggc gaaccaccgc gcacggacag ctcaccgacg
     2401 gcgaggcaga cctcgtgatc gcccacccgg agctcgggtt cctcgtcgtc gagacgaagg
     2461 caggcgagat ccggcgcgac gcgaccggtc gctggtatgc gggtagccgc atgctcgagc
     2521 cgagcccgtt cgaacaggcg aagcgaagcc ttcatgccct cctcggcaag ctcgccgagc
     2581 tgccggatcg gccgaccgac ttccatccga tcgccggcca cgcggtcgcc ctgccggacg
     2641 tcgacctcga gagcgccggt gctcgcctcc ggctcgccgg tccggacatc gtcgccggcc
     2701 tcgtcctcga ccgcgacgcg ctcccgcccg atgacccccc ggtcacgcgc gccgctgtcg
     2761 accgcgcgct cgacctctgg gcggccgacg cgccgaaccg gcgagcgccc gggaccgccg
     2821 gcgtccacct cctcgaggag atcctcgcga cgccggtcga gctgcgatcc ctgctccgaa
     2881 gcgagatcag cgccggcgag cggacggtcg tcgagctgac gaaccaccag atcgccgccc
     2941 tccgccggct gtccgcgatc cgccggatgc aggtcgtcgg cgcggccggg accggcaaga
     3001 cgctgctcgc cgcgaagaag gcggagatgc tcgcccgcga gggcttcgag acgctcctcg
     3061 tctgcttcaa ccagccgctc gcccgcctgc tcgcggagct caccgccgac acgactcgca
     3121 agcgcggccg cctgaccgtg tcgaccttcc accagctgtg cgaggacctc gcgaccgagg
     3181 ccggcgtcct cccgccgaag ccggagcccg tgacccagga ctggttcgac cggacgctgc
     3241 cggatgccct cctcgcggcg atcgacgtcc tcggctggcg cttccatgcg atcgtcatcg
     3301 acgagggcca ggacttcgcg cccgactggc tcgaggccct ccagctgctc ctcgaggact
     3361 cgcgcgagga cgtcctctac gtcttccacg atcccgccca ggcgatcttc cgcgacgaca
     3421 cggtcgagcg gctcgggctc gcgatgtatg cgctcgacga gaactgccgc aacccggggc
     3481 c
//
LOCUS       DATAIW010000139         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_36487, whole genome shotgun sequence.
ACCESSION   DATAIW010000139 DATAIW010000000
VERSION     DATAIW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36487"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VHM48_04490"
     CDS             <1..607
                     /locus_tag="VHM48_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2754694.1"
                     /translation="LVANCGSSARAGRPSARQNQAQNAFAGATPGRLANAHPNIVPYE
                     SFDTLDGAIAVAVGSERQWPRFCRALGLPALADDPQFATNGDRVTNRADLIPTLAARL
                     AERTTADWLAALDAADVPAGPINDIAAAFDSPWAAGATVELEHPRLGWIRQVAPPFEL
                     ARTPASIRTPPPLLGEHTDEILAELGYDADETIRLRADGIV"
     gene            670..1623
                     /locus_tag="VHM48_04495"
     CDS             670..1623
                     /locus_tag="VHM48_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754695.1"
                     /translation="MTVGTSRTSFRRIGALFLMGVVALAGCGGSGTPRPTDPRQILTN
                     AIAATASLATLRLHAEIAANAGAALGQGNAVMRAAIDADVDLATRQFAARVTTQTPAN
                     LSGNGVAQQQVSDTIVTRDATFNRNGQTGRWTKFPTGNGGGLSGGPTNAQIATMITSL
                     LSDPSQTFELKESASCTLGTCDHVIAHIDGPSLARALGGLLGAPLDASTGLTIPNLDV
                     DVLVDQSTSVISELRTGFTMTGTSLQILVTISNPGQPVQIAPPPPALTDDLGANFGGG
                     GFVGGGFATPMPIGPEESMILEQVGNELESSMPAEPEPSIP"
     gene            complement(1660..2421)
                     /locus_tag="VHM48_04500"
     CDS             complement(1660..2421)
                     /locus_tag="VHM48_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2754696.1"
                     /translation="MAGLGPLLRKELLEQWRTLRLPVIATIFLLVGLSSPLLARFTPE
                     IIKAVAGDQFPMAFPTPTAADAIDQFAKNIGQFGGLIAVLLAMGAVATEKERGTAALL
                     LTKPVSRAAFLIAKLVAIGLTLAVSVVIATAGAWFYTLVLFESLPPAGVAAGAVLQWL
                     MLMAWASITFVGSTLTRSSLAAAGLGIVAFIVVGILGVLPTVGQFLPTGLSGPARALA
                     LGVPGPDAIGPTVATMVLIGAAGSVAWLAFRRQEL"
     gene            complement(2421..3476)
                     /locus_tag="VHM48_04505"
     CDS             complement(2421..3476)
                     /locus_tag="VHM48_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754697.1"
                     /translation="MTTVVGGAGRSTATAGAPDAVAGIAIRARGLTKRFGSVVALDGL
                     DLDVPAGAIFGLLGPNGAGKTTTIRILTGLARQTAGTATVAGVEVGLDRPELRRRLGY
                     LDQDPRFYGWMRGRELLELVGRLVGLDGPALRERVGDMLERTGLTAAANRRIGGYSGG
                     MRQRLGIAQALLHRPTIVFLDEPVSSLDPEGRRDVLALIEGLRGETTVIVSTHVLSDV
                     ERICDRVAILDRGRLVTEAPLERLLADHIRPVYRLAPEPGQDAAVAAFIGRLRATPWV
                     TDVATAPEGDLRVSVNDPITAGPSLLAIVVDEGVRLAVFERVRPTLEDVFLELVGTPS
                     ADELDGRGFIRPREVGR"
     gene            complement(3473..3682)
                     /locus_tag="VHM48_04510"
     CDS             complement(3473..3682)
                     /locus_tag="VHM48_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2754698.1"
                     /translation="MPELKTEQILALLAPIVIIQLGLMIVALHDLEQDDRHVRGGSKL
                     IWALVIVFLNVLGPIVYLVAGREDA"
     gene            3988..>4388
                     /locus_tag="VHM48_04515"
     CDS             3988..>4388
                     /locus_tag="VHM48_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2754699.1"
                     /translation="MTDDRERWALILGASSGMGEATGLALAKAGYKIAGIHLDFRAAL
                     THVEEVKAAIVAAGSEALFVNMNAADDEKRAGALAQLNARFDESRAAGRKPYVRIVMH
                     SLAFGSLVPFLADDPKAAVDRKKMEMTQDVMA"
BASE COUNT          677 a         1621 c         1547 g          543 t
ORIGIN      
        1 gttggtggcg aactgcggat cgtcggcgag ggcgggcagg ccgagcgccc ggcagaacca
       61 ggcccagaac gcgttcgccg gggcgacccc gggccggctc gccaacgccc acccgaacat
      121 cgtcccgtac gagtcgttcg acaccctcga cggagcgatc gcggtggccg tcggcagcga
      181 gcgccagtgg ccgcggttct gccgggcgct cggcctgccc gccctcgccg acgatccgca
      241 gttcgccacc aacggcgatc gggtcacgaa ccgcgccgac ctgatcccga ccctggccgc
      301 gcgactcgcc gagcggacga ccgccgactg gctggccgcc ctcgacgccg ccgacgtccc
      361 ggccggcccg atcaacgaca tcgccgcggc cttcgactcg ccgtgggccg ccggtgcgac
      421 cgtggagctc gagcatcccc gcctcggctg gatccgccag gtcgcgccgc cgttcgagct
      481 cgcccggacg ccggcgtcga tccgcacgcc gccgccgctc ctcggcgagc acaccgacga
      541 gatcctcgcc gagctcggct acgacgccga cgagacgatt cgcttgcggg cagacggtat
      601 cgtctgagcc gacggtcgga cggggccacc cggaccggct cgcggcgacc gggaccttgg
      661 gaggggacca tgacggtcgg gaccagcagg acgtcgttcc gtcggatcgg ggcgctcttc
      721 ctcatgggcg tcgtcgccct cgcggggtgc ggtggctccg gcacgccgcg gccgaccgac
      781 ccgcgccaga tcctcacgaa cgcgatcgcg gcgaccgcgt cgctggcgac gctccgactc
      841 cacgccgaga tcgccgccaa cgcgggcgcc gcgctcggcc aggggaatgc ggtgatgagg
      901 gcggcgatcg atgcggacgt cgacctcgcg acccgccagt tcgcggcccg cgtgacgacc
      961 cagacgcccg cgaacctcag cggcaacggc gtggcgcagc agcaggtctc ggacacgatc
     1021 gtcacccggg atgccacctt caatcgcaac ggccagaccg gccgctggac gaagttcccg
     1081 acgggcaacg ggggcggcct cagcggcggc ccgacgaacg cccagatcgc gacgatgatc
     1141 acgagcctgc tgtctgaccc ctcgcagacc ttcgagctca aggagtcggc gtcctgcacc
     1201 ctcgggacgt gcgatcacgt catcgcccac atcgacgggc cgagcctcgc gagggccctc
     1261 ggcgggctcc tcggcgcccc gctcgacgcc agcacgggct tgacgatccc gaacctcgac
     1321 gtcgacgtcc tggtcgacca gtcgacctcg gtgatcagcg agctgcgcac cgggttcacg
     1381 atgaccggca cgtccctgca gatcctcgtg acgatcagca accccggtca gccggtccag
     1441 atcgcgccgc cgccgccggc cctgaccgac gacctcggcg cgaacttcgg gggcggcgga
     1501 ttcgtcggcg gcgggttcgc cacgccgatg ccgatcggtc cggaggagtc gatgatcctc
     1561 gagcaggtcg gcaacgagct cgagtcatcg atgccggccg aaccggagcc gtcgatcccc
     1621 tgagggtgac tcgcgcgccc catcccgtcg gagcgccggt cagagttcct gtcgacggaa
     1681 cgccagccag gcgaccgacc cggccgcgcc gatcaggacc atcgtcgcca ccgtcggccc
     1741 gatcgcgtcc ggtcccggaa ccccgagggc gagggcccgg gccgggccgc tgagcccggt
     1801 cggcaggaac tgcccgaccg tcggcaggac gccgaggatg ccgacgacga tgaaggcgac
     1861 gatgccgagg ccggccgccg caaggctcga ccgggtcagc gtgctcccga cgaacgtgat
     1921 cgaggcccag gccatgagca tgagccactg caggacggcg ccggccgcga cgccggccgg
     1981 cggcagggat tcgaacagga ccagcgtgta gaaccacgcc ccggccgtcg cgatcacgac
     2041 ggacaccgcg agggtcagcc cgatcgcgac cagcttggcg atcaggaacg ccgcccggct
     2101 gaccggcttg gtcaggagca gggcggccgt tccccgctcc ttctccgtgg cgacggcgcc
     2161 catcgcgagc aggaccgcga tcaggccgcc gaactggccg atgttcttgg cgaactggtc
     2221 gattgcgtcc gcggcggtcg gggtcgggaa cgccatcggg aactggtcgc cggccaccgc
     2281 tttgatgatc tcgggggtga accgggcgag gagcggggag ctcaggccga ccagcaggaa
     2341 gatcgtggcg atgactggca gcctcagcgt ccgccattgc tccaggagct ccttgcgcag
     2401 gagcggaccg aggccggcca tcaccgaccg acctcgcgcg gccggatgaa cccacggccg
     2461 tcgagctcgt ccgcactcgg cgtcccgacg agctcgagga agacgtcctc gagggtcggc
     2521 cggacccgct cgaacacggc cagccggacc ccctcgtcga cgacgatcgc cagcagcgac
     2581 gggccggcgg tgatcgggtc gttcacggac acgcgcaggt cgccttcggg cgcggtcgcc
     2641 acgtcggtca cccacggcgt cgcccgcaac cgcccgatga acgcggcgac cgcggcgtcc
     2701 tggcccggct cgggcgccag ccggtagaca ggtcggatgt gatcggcgag gagtcgttcg
     2761 agcggcgcct ccgtgacgag gcgcccgcga tcgaggatcg cgacgcggtc gcagatgcgc
     2821 tcgacatcgg acaggacgtg ggtcgatacg atcaccgtcg tctctccacg gaggccctcg
     2881 atcagggcga ggacgtcgcg tcgcccctcc ggatcgagcg agctcacggg ctcgtcgagg
     2941 aacacgatcg tcggccggtg gagaagggcc tgggcgatgc cgagacgctg tcgcatcccg
     3001 cccgagtagc cgccgatccg gcggttcgca gccgccgtga ggccggtccg ctcgagcatg
     3061 tcgccgaccc gctcccgcag cgcgggtcca tcgaggccga cgagccggcc gaccagctcg
     3121 agcagctcgc ggccgcgcat ccagccatag aagcgtgggt cctggtcgag gtagccgagc
     3181 cgccggcgca gctccggtcg atcgagcccg acctcgacgc cggcgacggt tgcggtcccg
     3241 gcggtctggc gggcgagacc ggtcaggatc cggatcgtcg tcgtcttgcc ggcgccgttc
     3301 ggcccgagga gcccgaagat cgcgccggcg ggcacatcga gatcgaggcc gtcgagggcg
     3361 acgaccgagc cgaaccgctt ggtcagcccg cgggcgcgga tcgcgatccc ggcgaccgcg
     3421 tcaggggcac cggcggtggc tgtcgaccgg cccgcgccac cgacgacggt cgtcacgcgt
     3481 cctcccgccc ggcgacgaga tagacgatcg gtccgaggac gttgaggaac acgatcacga
     3541 gcgcccagat gagcttgctg ccgccgcgga catggcggtc gtcctgctcg aggtcgtgga
     3601 gagccacgat catcaacccg agctggatga tcacgatcgg ggcgagcagg gcgaggatct
     3661 gttcggtctt caattcaggc acagctggct cagggtacgc gcggacggat cgccgggtat
     3721 cggtccggcc ggcccgttcg gcgcaggccg atccacccga acgcttgcac ggctgtgacc
     3781 cggccggcgc gccccatcga gcgacatcca atcagccgtc cgccgccgcc gtccgccgcc
     3841 gtctcgcgca cccccacccc tgcacgcctg cacgcctgca ccgaacgtgc ccggttcgtg
     3901 ccgggaccgc tgctagactc cgcgggccgc tccattccgc ggtaacggac agcccgacgc
     3961 acgccgccac gcacccggag gaacccgatg accgacgatc gcgagcgctg ggcgctcatc
     4021 ctcggcgcct cgagcggcat gggggaggcg accgggctgg ccctcgcgaa ggccggctac
     4081 aagatcgcgg gcatccacct cgacttccgg gccgccctga cccacgtcga ggaggtcaag
     4141 gcggcgatcg tcgcggccgg cagcgaagcg ctgttcgtca acatgaacgc ggccgacgac
     4201 gagaagcggg caggcgcgct cgcccagttg aacgcccgct tcgacgagag ccgggccgcg
     4261 ggccggaagc cctacgtccg gatcgtgatg cattccctcg cgttcgggtc gctcgttccg
     4321 ttcctcgccg acgacccgaa ggccgcggtc gaccgcaaga agatggagat gacccaggac
     4381 gtcatggc
//
LOCUS       DATAIW010000140         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_181950, whole genome shotgun sequence.
ACCESSION   DATAIW010000140 DATAIW010000000
VERSION     DATAIW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181950"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VHM48_04520"
     CDS             complement(<1..958)
                     /locus_tag="VHM48_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754700.1"
                     /translation="MRNDWDAIVVGLGAIGSGTAYWLSRRLGDRVLGLEQFELGHTNG
                     AGQDHSRIIRYSYHRPDYVRLARRAYEAWAEVEAESGTRIVTRTGGLDLGPRVAAIPL
                     EDYTRSMTEEGIAFELLDGPEISRRWPQWRLGDEHHGLYQADAGIADPNKGKAAHQRL
                     ARAHGATLLERTPVARMRELAGGGIEVELADGTVHRAPHVDLAADAWTNDLLASFDRR
                     LPLTIIQAQVTYFAAPDPALFAPDRFPIWIWMDDPSFYGFPTYGEAGPKAAEDVGGDE
                     VTPATRTFERNEVGFRRLTDFLARHLPEHLGPEIVTKTCLYTL"
     gene            1048..1410
                     /locus_tag="VHM48_04525"
     CDS             1048..1410
                     /locus_tag="VHM48_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2754701.1"
                     /translation="MLRVGSVVIRVDDLARQAAFWAAALDYVSREDPNAAGADFALLH
                     PRDGDGPNVSLDRVRSALHIPPRIHLDLYAEDQAAEVERLKTLGATEIHWDKRPVDAD
                     YVIMADPEGNRFCVIDAR"
     gene            1541..1801
                     /locus_tag="VHM48_04530"
     CDS             1541..1801
                     /locus_tag="VHM48_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754702.1"
                     /translation="MNVRTNLLLPKELVEEVDHFAGERGRSRYVAEALRARLKRDRLK
                     EAMERTAGVWTAEEYPEFATSEKVVEWVRARRAEVTDPGQDA"
     gene            1794..2186
                     /locus_tag="VHM48_04535"
     CDS             1794..2186
                     /locus_tag="VHM48_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2754703.1"
                     /translation="MPEPMRYLIDTTFVIDHLRGDRATLELMARLVEHGDGLFVNEVV
                     VAESWSGAHLDDDPDLEAFLEFIEFVQPGPDAARAAGRWRADARRRGWTLSMTDALIA
                     AAAQSLDAVLLTRNLRDFALTPVRVQTY"
     gene            complement(2180..2860)
                     /locus_tag="VHM48_04540"
     CDS             complement(2180..2860)
                     /locus_tag="VHM48_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX2754704.1"
                     /translation="MTTLTVLRVFVGPDGRGGNPLGVFLDGAAIPPDRRQAVAVDLGF
                     SETVFVDDAAEGAIRIFTPGRELPFAGHPTVGTGWLFRETGAPATTLRPPAGDVPFRH
                     DAERTWVRARPEWVHPILVEQLATAADVEAHPGQPMGVSGRYVWAWIDEAAGTLRSRY
                     FATDVGIREDEATGAAAVVMGGILGRPITIRQGVGSEILVQPQPDGTVEIGGRVEMVE
                     AREYELPQ"
     gene            complement(2857..3453)
                     /locus_tag="VHM48_04545"
     CDS             complement(2857..3453)
                     /locus_tag="VHM48_04545"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2754705.1"
                     /translation="MSDVDSELAERVGPWTRRSRRVAYDNPWITVWHDDVDRPDGSAG
                     IYGVVHFANLAVGAVILDDDDRVLLVGQHRYTLDEYSWELPEGGVPEGESAIDGAARE
                     LREETGVEAGDWREILRFGLSNSISDEVGVIFAARATSHGEASPDPTEELAVRWLPFD
                     DALAMTAEGRITDAITIMGLQRVALERAQVQSAKEVPG"
     gene            3520..5616
                     /locus_tag="VHM48_04550"
     CDS             3520..5616
                     /locus_tag="VHM48_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX2754706.1"
                     /translation="MQDPVDVERLRPASQPVHRVAALRDVRIPMSDGLELSANLWLPE
                     PLADGTDPQAFPAILEMIPYRKDDWRAASDESRGEWLAARGFALCRLDIRGTGSSPGI
                     ALDEYTARETQDGYDAVEWLAAQSWCNGKVGMWGISYGGFTSIQVALLDPPHLAAIVP
                     MMATDDRYTDDVHYLGGCTTVSELSQYAVSMVGMNAMPPRAEYRGAGWATEWRERLDE
                     TPIWLFEWLRQQHDGPYWRQGSLAPHWERLTTPTFVIGGWMDEYVDAALRMLERCTNA
                     PRRALIGNWVHDLPDDAYPGPNVDWYHEMVRFFDHWLKGIDNGVMEEPGLVAFRHEWA
                     EPEPFPAAWPGEWIGGEAAWPPADIEWLDLHLASSEAPLVGHLSDRPPTEPSVERFRH
                     RATIGTRAGLSWGAGGRPNGVARDLRPDDALGPTFTSEPLGADLDVVGVASVVLEWES
                     PVPVATAVVRLQDLAPDGTPFQVSAGILNLTHRTSHESPEALEPGVVTEVRIALRSTA
                     HRFRAGHRIRLSVASSMWPVVWPSPFPAEYGLHLGGDPAAASRLILPTLPAAHLGAAV
                     PPFKTTPAGLREVGSYRGDPPVWRVIDDVMDGSVTVETSEFGESTLPDGRTTLYTGER
                     LEMTARDADPAHARMHNEVVYRLREDGSEILIEANGTIRTTETDFHMNVGLTVTLDGA
                     PFFERGWLETIPRRLV"
     gene            complement(6076..>6518)
                     /locus_tag="VHM48_04555"
     CDS             complement(6076..>6518)
                     /locus_tag="VHM48_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754707.1"
                     /translation="TEPTPRDPSANVPTPRDPSANVPASTTRRWSIRLGPLDARVTAE
                     PDPAPAPAAPLGPSASVPAPLGTSARVGSTIGILERILIVVFVLTGTDAAIGFVVAAK
                     TLARFKLLDDRDFAEYYLLGTLASVSVAIVTALVGRAALTVLLA"
BASE COUNT          955 a         2282 c         2316 g          965 t
ORIGIN      
        1 tcaaggtgta gagacacgtc ttcgtgacga tctccggccc gagatgctcc gggaggtggc
       61 gcgccaggaa gtcggtcagg cggcggaacc cgacctcatt gcgctcgaag gtccgggtcg
      121 cgggcgtgac ctcgtcgcca ccgacgtcct ccgctgcctt cggcccggcc tcgccgtagg
      181 tcgggaagcc gtagaacgag gggtcgtcca tccagatcca gatcgggaag cggtccgggg
      241 cgaacagggc gggatccggc gcggcgaagt aggtgacctg ggcctggatg atcgtcaacg
      301 gcaggcgccg gtcgaaggac gcgagcaggt cgttcgtcca ggcgtcggcg gcgaggtcga
      361 cgtgcggcgc ccggtggacg gtgccgtccg cgagctcgac ctcgatcccg ccgccggcga
      421 gctcgcgcat ccgcgccacc ggcgtgcgtt cgagcaacgt tgccccgtga gcccgggcga
      481 ggcgctggtg ggccgctttg cctttgttcg ggtcggcgat cccggcgtcg gcctggtaca
      541 ggccgtgatg ctcgtcaccg agccgccact gcggccagcg ccggctgatc tccggaccgt
      601 cgaggagctc gaaggcgatg ccctcctccg tcatggagcg ggtgtagtcc tcgagcggga
      661 tggcggcgac acgcgggccg aggtcgaggc caccggtcct cgtcacgatc cgcgttccgc
      721 tttcggcctc gacctccgcc caggcctcgt aggcgcgacg ggcaaggcgg acatagtccg
      781 gccggtgata cgagtagcgg atgatccggc tgtggtcctg gcccgccccg ttggtgtggc
      841 cgagctcgaa ctgctcgagg ccgaggaccc ggtcgccgag ccgccggctc agccagtagg
      901 ccgtaccgga gccgatcgcc ccgaggccca ccacgatcgc gtcccagtcg ttccgcacgc
      961 tccgatgcta gtcgcgacga ctccgggaat gttggctcgt cgcgcatcgc gtcgacccgc
     1021 ttcgcgtcgc gcgggtatcg tgaatcgatg ctccgcgtcg gttcggtcgt gattcgggtc
     1081 gatgaccttg cgcgccaggc tgccttctgg gcggcggccc tcgactacgt ctctcgcgag
     1141 gatccgaacg cggccggggc cgacttcgcc ctgctccacc cgagggacgg cgacggcccg
     1201 aacgtctcgc tcgaccgcgt tcgatcggcg ctccacattc cgccgcgcat ccacctcgac
     1261 ctctacgccg aggaccaggc cgccgaggtc gaacggctca agacgctcgg cgcgaccgag
     1321 atccactggg acaagcggcc tgtcgacgcc gattacgtga tcatggcgga cccggaaggc
     1381 aaccggttct gcgtgatcga cgccaggtag gcgttttcgg cgccgcgcat gacccgccgg
     1441 gggccggttc gggcggggga cgaccgaacc gccggaggcc tggtcttcaa gggcgcccag
     1501 actccttgac gtgccgacgg tcgtgcgcca tgctgtgcgc atgaacgtgc gcacgaacct
     1561 gctgctgccg aaggagctcg tcgaggaagt cgaccatttc gccggtgagc gcggccggag
     1621 ccgctacgtg gcggaggcgc ttcgggcccg gctcaagcgc gaccgcctga aggaggcgat
     1681 ggagcggacg gcgggggtgt ggacggccga ggagtacccg gaattcgcga cttcggagaa
     1741 agtcgtggaa tgggtcaggg cccggcgagc cgaagtcacg gatcccggcc aggatgcctg
     1801 agccgatgcg ctacctgatc gacaccacgt tcgtcatcga ccacctccgc ggcgatcggg
     1861 cgacgctgga attgatggct cgcctcgtcg aacatggcga tgggctcttc gtgaacgagg
     1921 tcgtggtcgc cgagtcgtgg tccggcgctc atctcgacga cgaccccgat ctggaggcgt
     1981 tcctcgagtt catcgagttc gttcagccgg gaccggacgc ggctcgcgcc gcgggccgct
     2041 ggcgggccga tgcccgccgg cgcggatgga ctctcagcat gacggacgcg ctgatcgcgg
     2101 ccgcggcgca atcgctggac gccgtgctcc tcacgaggaa tctgcgcgac ttcgcgttga
     2161 cacctgtccg cgtccagacc tactgaggca gctcgtactc gcgggcctcg accatctcga
     2221 cccgaccgcc gatctcgacc gtgccgtcgg gctgcggctg gacgaggatc tcggagccga
     2281 caccctgacg gatcgtgatc ggccggccga ggatgccgcc catgacgacg gccgcggcgc
     2341 cggtcgcctc gtcctccctg atcccgacgt cggtggcgaa gtagcgcgac cggagggtac
     2401 ccgccgcctc gtcgatccag gcccacacgt agcgacccga cacgcccatg ggctggcccg
     2461 gatgggcttc gacatcggca gcggtcgcga gctgctcgac gaggatcggg tggacccact
     2521 ccggtcgggc ccggacccag gtccgctcgg cgtcgtgccg gaacgggacg tcaccggccg
     2581 gcgggcggag cgtcgtcgcc ggcgcaccgg tctcgcggaa gagccagccg gtgccgacgg
     2641 tcggatggcc ggcgaacggc agctcgcggc ccggggtgaa gatccggatc gcgccctcgg
     2701 ccgcgtcatc gacgaacacg gtctcggaga agcccaggtc gacggcgacc gcctggcggc
     2761 gatcgggcgg gatcgccgcg ccgtcgagga agacgccgag cggattgcca ccgcgaccgt
     2821 ccggtccgac gaagacccgc aggaccgtga gcgtcgtcat ccggggacct ccttcgcgga
     2881 ctggacctgg gcccgttcga gggccacgcg ctggaggccc atgatcgtga tcgcgtcggt
     2941 gatccggccc tcggccgtca tcgccagcgc gtcgtcgaac gggagccagc gaacggcgag
     3001 ctcttcggtc gggtcgggcg acgcttcgcc gtggctcgtg gcccgggccg cgaagatgac
     3061 cccgacctcg tcgctgatcg aattcgacag gccgaaccgg aggatctcgc gccagtcgcc
     3121 agcctccacc ccggtctcct ctcgcagctc tcgggctgcg ccgtcgatcg ccgactcccc
     3181 ttcgggcaca ccgccctcgg gcagctccca cgagtactcg tcgagcgtgt agcggtgctg
     3241 gccgaccagc agcacccgat cgtcgtcgtc gaggatcacg gcaccgacgg cgaggttcgc
     3301 gaagtggacg acgccgtaga tgccggcgct gccgtcgggc cgatcgacgt cgtcatgcca
     3361 gacggtgatc cacgggttgt cgtaggcgac ccggcgcgac cgtcgcgtcc atggaccgac
     3421 acgctcggcc agctcggagt cgacgtcgct catggcagca tcgtcgcacg gcccgggcga
     3481 cggggtggcg cggcttggac cggcgcctat cctcggggca tgcaggatcc cgtcgacgtc
     3541 gagcgcctcc gaccggcgag ccaaccggtg catcgtgtcg cggcactgcg ggatgtccgg
     3601 atccccatgt cggacgggct cgagctgtcg gcgaacctgt ggctgccgga gccgctggcg
     3661 gacgggacgg acccgcaggc gtttcccgcg atcctcgaga tgatcccgta ccgcaaggac
     3721 gattggcgcg cggcgagcga cgagagccgc ggcgagtggc ttgcggcgcg cggcttcgcc
     3781 ttgtgccggc tcgacatccg cggcacgggc tcgtcgcccg ggatcgccct cgacgagtac
     3841 accgcccgcg agacccagga cggctacgac gcggtcgaat ggctcgccgc ccaatcctgg
     3901 tgcaacggca aggtcgggat gtggggcatc tcgtacggcg gcttcacctc gatccaggtc
     3961 gcgctgctgg acccaccgca cctcgccgcg atcgtcccga tgatggccac cgacgatcgt
     4021 tacaccgacg acgtccacta cctgggcggc tgcacgacag tgtcggagct ctcccagtac
     4081 gcggtgagca tggtcgggat gaatgccatg ccgccgcggg cggaataccg cggcgcgggc
     4141 tgggccacgg aatggcgcga gcgcctggac gagaccccga tctggctgtt cgagtggctc
     4201 cgccagcagc acgacgggcc gtactggcga cagggctcgc tcgccccgca ctgggaacgc
     4261 ctgacgacgc cgaccttcgt gatcggcggc tggatggacg agtacgtcga cgcggccctc
     4321 cggatgctcg agcgctgcac gaacgcgccg cgtcgggcgc tgatcggcaa ctgggtccac
     4381 gacctgcccg acgacgccta ccccgggccg aacgtcgact ggtaccacga gatggtccgc
     4441 ttcttcgacc actggctgaa gggcatcgac aacggggtca tggaggagcc gggtctcgtc
     4501 gcgttccgcc acgagtgggc cgagcccgag ccgttcccgg cggcctggcc gggcgagtgg
     4561 atcggcggcg aggccgcatg gccgccggcg gatatcgagt ggttggacct ccatctggca
     4621 agcagcgagg cgccgctggt cgggcatctg agcgatcggc cgccgaccga accgagcgtc
     4681 gagcggtttc gccatcgagc gacgatcggg acgcgggcag gcctgtcgtg gggcgccggc
     4741 ggccggccga acggcgtcgc ccgcgacctt cgcccggacg acgccctggg accgacgttc
     4801 acctcggagc cgctcggcgc cgacctcgac gtggtcggcg tcgcctccgt ggtcctcgaa
     4861 tgggagtcgc cggtgcccgt cgccacggca gtcgtccggc tccaggacct cgccccggac
     4921 gggacgccgt tccaggtcag cgccgggatc ctgaacctga cccaccgcac atcgcacgag
     4981 tcgccggaag ccctcgagcc gggtgtcgtg actgaggttc ggatcgccct ccgctcgacg
     5041 gcccatcgat ttcgggctgg tcatcgcatc cggttgtcgg tcgcctcgtc gatgtggccg
     5101 gtcgtctggc cgtcgccctt cccggcggag tacgggctgc atctcggcgg cgacccggcc
     5161 gccgcctccc ggctgatcct gccgaccctt cctgccgccc acctcggggc cgccgtcccg
     5221 ccgttcaaga ccaccccggc cggccttcgc gaggtcggct cgtaccgggg tgacccaccg
     5281 gtctggcgcg tgatcgacga cgtcatggac ggctcggtga ccgtcgagac cagcgagttc
     5341 ggcgagtcga cgctgcccga tggccggacg acgctctaca ccggcgagcg cctcgagatg
     5401 accgcccgcg acgccgaccc ggcccacgcc cggatgcaca acgaggtcgt ctaccgcctc
     5461 cgcgaggacg gctcggagat cctcatcgag gcgaacggca cgatccggac gaccgagacc
     5521 gacttccaca tgaacgtcgg gctcacggtg accctcgatg gcgccccctt cttcgagcgc
     5581 ggctggctgg agacgatccc gcgccgcctc gtctgaccgt cggatgtcga acttggctcg
     5641 ccagatgaac gattccagca atcgcgttca ttggatgaac caatcgaacg agcgaggcac
     5701 gatggacact cgatccggga cccatcgatg tccgggccgg ccggaaacga ccgttctcgc
     5761 tcttctgatg acgcatctcg tgcaggtcag gagcgccgac cgatcgagcg ggtcacatcg
     5821 gttcattgga tgaacggggc tctcgctcaa tccccgggga cgtgcttctc cagctcctcg
     5881 aggaagtgct tcgcgccacc cgccatcgtg taccgggtcg ccggctcgtg ctcgaattgg
     5941 cgatcattcg tcggatcaca gccaacgaga cctccgtcac ccgccgtcac ggtgaccgtg
     6001 atggactccg gctggccgag ggcgtcaccc gcatcggccc ggccagtcgt cgagagccgc
     6061 cgttcgacgg tgaggtcagg ccaacagcac cgtcagcgcg gctcgcccga ccagcgccgt
     6121 gacgatcgcg accgagacgc tggccagggt gccgaggagg tagtactccg cgaagtcgcg
     6181 gtcgtcgagg agcttgaacc gggcgagggt cttggcggcg acgacgaacc cgatcgccgc
     6241 gtccgttccg gtcaggacga agacgacgat caggatccgc tcgaggatcc cgatggtcga
     6301 cccgacgcgg gcgctcgtcc cgaggggcgc cggcaccgag gccgacggac cgagcggcgc
     6361 cgccggcgcc ggagcgggat cgggttcggc ggtgacccgc gcgtcaagcg gcccgagccg
     6421 gatcgaccag cgacgggtcg tggacgccgg cacgttcgcg gacggatcgc ggggcgtcgg
     6481 cacgttcgcg gacggatcgc ggggcgtcgg ctcggtcg
//
LOCUS       DATAIW010000141         5132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_241431, whole genome shotgun sequence.
ACCESSION   DATAIW010000141 DATAIW010000000
VERSION     DATAIW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241431"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VHM48_04560"
     CDS             <1..492
                     /locus_tag="VHM48_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2754708.1"
                     /translation="TPIIAAVRGFALGGGCELALSCDLIVAGDDAQFGQPEINLGVMP
                     GAGGTQRLTRAIGRARAMDLVLTGRTITAREAEATGLVSRVVPADQTLDAALELAATI
                     ASKAPIAVLAAKEAVRLADELPLSAGLRHERRAFFALFASDDQREGMSAFIEKRRPEW
                     KGR"
     gene            496..1311
                     /locus_tag="VHM48_04565"
     CDS             496..1311
                     /locus_tag="VHM48_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754709.1"
                     /translation="MERDGAGGSDHGRDDVRDLGDEPPASFRDTAPDVLAPIDISSPV
                     SGHVPGQHPLHETAASIAESPEHDWGAAGALLFPLLRPSGTQGMAVTDIDATALAADT
                     MRSHSQPLVDEGPCGLSVVYAIPGSGFDVIVNGDHLLSWGVTIEAIQDTALGNLAAWS
                     AAAAWTDEVSGERRLISSDTGDGWDASRILLPEVRDRLVAELGGAGRVLIGLPERHLM
                     IAGALRPGDDEFAGLFGEFVLEQSGAADEPVDRRIFELVDGRLVEFRGLPLPA"
     gene            1320..2096
                     /locus_tag="VHM48_04570"
     CDS             1320..2096
                     /locus_tag="VHM48_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2754710.1"
                     /translation="MADLRLETVDAVATITLDRPDALNALTVPLKEDLLAALAEVAAD
                     DAVRAVVLTGAGRAFCAGQDLRERLEPDAAPLAVEIRVRYNPLIRAMRGLPKPIVGAI
                     NGIAAGAGASLAFACDIRIAAEGASFLLAFGRVGLIPDTGATWFLPRLVGGAKAVELA
                     LTTDPLSAVDAERFGLVARVVPGDTLLAEAQALAARLAAGAPRALALTKRALEASWSA
                     TLDEQLELEADVQGEAGATADHAEGIAAFLERRPARFSGE"
     gene            complement(2188..3504)
                     /locus_tag="VHM48_04575"
     CDS             complement(2188..3504)
                     /locus_tag="VHM48_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX2754711.1"
                     /translation="MMAQPLTILFMPESAYGPTNNCIGIGDVLRKRGHTVVFAAEASW
                     NGRLAPLGFVEDLVDLSPPPPPDAPAQDAGQFWKDFIRDTAPEFRKPTIEQLATFMQP
                     TWQALIDGAKYCEPQLREIIARNEPDVIVEDNVVSFPALQTAGKPFVRIVSCNPLEVR
                     GLAGHEIPPPYSGLPLDDRSQWEAFRQEYDRTHRPTWEDFDAWCRDQGTAPLPELEFE
                     HTSRHLNLYVYPEVADYADRRPLDRTWRRLDSSVRATDGDVELPAAMADRPSGAELIY
                     LSLGSLGSADVSLMRRLVAVLADTPHRYIVSKGPQHADYDLAPNMWGAEFLPQTKIVP
                     LVDLVITHGGNNTTTEAFHFGKPMIVLPLFWDQYDNAQRVDETGFGVRLPTYAFADDD
                     LRGAIDRLLGDADLRGRMARTGAEIRAKDGVAKAADAIEATGLAGR"
     gene            3511..4815
                     /locus_tag="VHM48_04580"
     CDS             3511..4815
                     /locus_tag="VHM48_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754712.1"
                     /translation="MTERTDETVPGIAASSYAADERLLAELRAAWPPAPPAAADEARG
                     YVAADLVDREPSAALRLGDRFAVALMPGAGGSFDVVPLARDELVVSTWRHARAGDGLS
                     AFIAGVPMASERAIEADQTNASVVVGERAIVKWFRRVGPEPSRAATLIAHLDAVGFAE
                     MPAPLGSLVWPSPAGVELTIAQGDAFLPSARDGWEWAVERLERHVDHGETDCPADCDP
                     WIGARLGQLVARLHAALRTPSAAIPVAIATASPAVVDGWRAAAVASLDDAVRLTAEQD
                     VAGAALLAAIEPALRADLDRLRVERDLELQPIHGDLHVGQVLAWAGGLALIDFDGNPT
                     LAADANALRQPGERDIAQMMMSLDHVARIVDHRSAGHARAIVAAWIATTRREFLGAMD
                     SDPVILAAFEVEQECRELVYAARFLPRWRYAPLATLRARYGT"
     gene            4842..5066
                     /locus_tag="VHM48_04585"
     CDS             4842..5066
                     /locus_tag="VHM48_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754713.1"
                     /translation="MNPLQFLADLETRPQWLGLLADRFARAIRSRPCRATSSRAEVLV
                     AELLGATWWRRPVLERARVGNKPVTNRSHS"
BASE COUNT          700 a         1842 c         1846 g          744 t
ORIGIN      
        1 accccgatca tcgccgcagt ccgcggcttc gccctcggcg gcggctgcga gctggcgttg
       61 agctgcgacc tgatcgtcgc cggcgacgac gcccagttcg gccagcccga gatcaacctc
      121 ggcgtgatgc ccggtgccgg cggcacgcaa cgactgacgc gggcgatcgg tcgggcccgg
      181 gcgatggacc tcgtcctgac gggccggacg atcacggccc gcgaggccga ggcgacggga
      241 ctggtcagtc gggtggttcc cgccgaccag accctcgacg ccgccctgga gctggccgcc
      301 acgatcgcga gcaaggcgcc gatcgccgtg ctcgccgcca aggaggccgt caggctggcc
      361 gacgagctgc cgctctcggc cgggctgcga cacgagcgcc gggcgttctt cgccctcttc
      421 gccagcgatg atcaacggga aggaatgtcg gccttcatcg agaagcgccg gccggagtgg
      481 aagggacgct gaccaatgga acgtgatggg gcgggtggga gcgaccacgg tcgcgacgac
      541 gtccgcgacc tcggcgacga gccgccggcg agcttccgcg acacggcccc ggacgtgctc
      601 gccccgatcg acatctcctc gcccgtgtcg ggccacgtcc cgggccagca tccccttcac
      661 gaaacggccg cctcgattgc agaatcgccc gagcatgact ggggggccgc cggcgcgctc
      721 ctcttcccgt tgctccgccc gagcggaacg cagggcatgg cggtcacgga tatcgatgcg
      781 accgccctcg cggccgacac gatgcggagc cactcccagc cgctcgtcga cgaaggcccg
      841 tgcgggctct cggtcgtcta tgcgatcccg ggcagcggct tcgacgtgat cgtcaacggc
      901 gaccacctcc tctcgtgggg tgtcacgatc gaggcgatcc aggatactgc gctgggcaat
      961 ctcgccgcct ggtcggcggc agccgcgtgg accgatgaag tctcgggcga gcggcgtctg
     1021 atctcgtcgg acaccggcga tggctgggat gcctcgcgga tcctcctgcc cgaggtccgt
     1081 gaccgactcg tggcggagct cggcggggcc gggcgagtcc tgatcggcct gcccgagcga
     1141 cacctgatga tcgccggagc gctgcggccc ggcgacgacg agttcgccgg cctgttcggc
     1201 gagttcgtcc tggaacaatc gggcgcggcc gacgaaccgg tcgaccgccg gatcttcgag
     1261 ctcgtcgacg ggcggctggt cgagttccgc ggcctcccgc tgcccgcctg agccgatcct
     1321 tggcggacct tcgactcgag accgtcgacg cggtcgcgac gatcaccctg gatcgtcccg
     1381 acgcgctcaa cgccctgacc gtcccgctga aggaggacct cctcgcggca ctcgccgaag
     1441 tcgcggccga cgacgccgtc cgggccgtcg tcctgaccgg ggccggacgg gcgttctgtg
     1501 ccggccagga cctgcgcgaa cgtctggagc cggacgccgc accgctcgcg gtcgagatcc
     1561 gcgttcgcta caacccgctg atccgggcga tgcgtgggtt gcccaagccg atcgtcggcg
     1621 cgatcaacgg aatcgccgcc ggtgccggcg cgtcgctcgc cttcgcctgc gacatccgga
     1681 tcgccgccga gggagccagc ttcctgctcg ccttcgggcg ggtcggcctc atcccggata
     1741 ccggtgcgac gtggttcctg ccgcggctcg tcgggggcgc caaggcggtg gagctggcgc
     1801 tgacgacgga tcccctgagc gcggtcgatg ctgagcggtt cggtctggtc gcgcgggtgg
     1861 tccccggcga caccctcctc gccgaggcgc aggccctggc cgctcgcctg gccgcgggtg
     1921 cgccgcgcgc gctcgcgctg acgaagcggg ccctcgaagc gagctggagc gcgacgctcg
     1981 acgaacagct cgagctggag gcggatgtcc agggcgaggc cggtgcgacc gccgatcacg
     2041 ccgaggggat cgccgcattc ctcgaacgac ggccagcgcg gttctccggg gagtagccga
     2101 ggcacggcag ggccgtcccg acgacagacc ttgaccggga tcgggcacgg gccgcgccgg
     2161 gacacgagga cgaggacgcg gcgtcggtca ccggcccgcc aaccccgtcg cctcgatcgc
     2221 atccgcggcc ttcgcgaccc cgtcctttgc ccggatctcc gccccggtcc gggccatccg
     2281 gccgcgcagg tcggcatcgc cgagcagccg gtcgatggca ccccggagat cgtcgtcggc
     2341 gaacgcgtac gtcggcagcc ggacgccgaa cccggtctcg tcgacccgct gggcgttgtc
     2401 gtactggtcc cagaagaggg gcaggacgat catcggcttg ccgaagtgga aggcctcggt
     2461 cgtcgtgttg ttgccgccgt gggtgatcac gagatccacc agcggcacga tcttcgtctg
     2521 gggcaggaac tccgcgcccc acatgttcgg ggccaggtcg tagtcggcgt gctgcgggcc
     2581 cttcgagacg atgtaccgat ggggcgtgtc ggcgaggacc gcgaccagtc gccgcatgag
     2641 cgacacgtcg gcgctgccga gcgacccgag cgacaggtag atcagctcgg cgccggacgg
     2701 ccggtccgcc atcgccgcgg gcagctcgac gtcgccgtcg gttgcccgga ccgacgaatc
     2761 gagccgccgc caggtccggt cgagtgggcg ccgatcggcg tagtcggcga cctccgggta
     2821 gacatacagg ttcaggtgcc gcgacgtgtg ctcgaactcg agctccggca gcggcgccgt
     2881 gccctggtcg cggcaccagg cgtcgaagtc ctcccaggtc ggacggtggg tccggtcgta
     2941 ctcctggcga aacgcttccc actggctgcg atcgtcgagc ggcaaccccg aatacggagg
     3001 cgggatctca tgcccggcca gcccgcggac ctcgagcgga ttgcacgaca cgatccggac
     3061 gaacggcttg cccgcggtct ggagcgccgg aaacgagacg acgttgtcct cgacgatcac
     3121 gtccggctcg ttgcgggcga tgatctcgcg gagttgcggc tcgcaatact tcgcgccgtc
     3181 gatcagggcc tgccaggtcg gctgcatgaa ggtcgcgagc tgctcgatcg tcggcttgcg
     3241 gaactccggc gcggtgtcgc ggatgaagtc cttccagaac tggccggcgt cctgggcggg
     3301 cgcgtcgggc ggcggtggcg gcgacaggtc gacgaggtcc tcgacgaacc cgagcggcgc
     3361 caggcggccg ttccacgagg cctcggcggc gaagacgacc gtgtggccgc gcttgcggag
     3421 gacgtcgccg atcccgatgc agttgttcgt cgggccgtaa gccgactcgg gcatgaacag
     3481 gatggtcagc ggctgggcca tcatctcctc gtgactgagc gaaccgacga gaccgtgccg
     3541 gggatcgcgg cgtcatcgta cgcggcggac gagcgcctcc tcgccgagtt gcgcgcggcg
     3601 tggccgccgg cgccgccggc cgcggcggac gaagcccgcg gctacgtcgc ggccgatctc
     3661 gtcgaccgcg agccctccgc cgcgctccgc ctcggggatc ggttcgccgt ggccttgatg
     3721 cccggtgcgg gtggttcctt cgacgtcgtg ccgctggccc gcgacgagct cgtcgtcagc
     3781 acgtggcgtc acgcccgagc cggcgacggc ctgtcagcgt tcattgccgg ggtgccgatg
     3841 gcctccgaac gggcgatcga ggccgaccag acgaacgcct cggtcgtcgt cggcgaacgg
     3901 gcgatcgtca agtggttccg gcgggtcgga ccggagccgt cgcgggcagc caccctgatc
     3961 gcccacctcg acgcggtcgg gttcgccgag atgcccgccc cgctcgggtc cctggtgtgg
     4021 ccgtcgccag ccggggtcga gctgacgatc gcccagggcg acgccttcct gccgagcgcc
     4081 cgggacggct gggaatgggc ggtcgagcgg ctggagcggc acgtcgacca tggcgagacc
     4141 gactgtccgg ccgactgcga cccgtggatc ggggcgcgac tcggacaact cgtggcgcgg
     4201 ttgcacgctg cgctgcggac cccgtcggcg gcgatcccgg tcgcgatcgc gaccgcctcc
     4261 cctgccgtcg tcgacggctg gcgcgccgcg gcggttgcgt ccctcgacga cgccgtgcgg
     4321 ctgacggcgg aacaggacgt cgcaggcgcg gcgctccttg ccgccatcga gccggcgctg
     4381 cgagccgacc tcgaccggct ccgagtcgag cgcgacctgg agctccagcc gatccacggc
     4441 gacctccacg tcggacaggt cctcgcgtgg gcgggcgggc tcgcgctgat cgacttcgac
     4501 ggcaacccga ccctcgccgc cgacgccaac gcccttcgcc agcccggcga gcgggacatc
     4561 gcccagatga tgatgagcct cgaccatgtc gctcgaatcg tcgaccatcg ctcggccgga
     4621 catgcccgcg cgatcgtcgc ggcctggatc gccacgaccc gccgcgagtt cctcggcgcc
     4681 atggactccg atcccgtgat tcttgccgca ttcgaggtcg agcaggaatg ccgtgagctc
     4741 gtctacgccg cccggttcct gccgcgctgg cggtacgcgc ccctggccac gctccgtgcc
     4801 cgctacggga cgtgaacagc cgccatggga ccattgcggc gatgaacccg ctccaattcc
     4861 tcgccgacct cgagaccagg ccgcagtggc tgggcttgct cgccgatcgg ttcgcccggg
     4921 caatccgttc gcggccgtgc cgcgcgacgt cgagccgggc cgaggtcctc gtcgcggagc
     4981 tgctcggcgc gacctggtgg cgccgacccg tgctcgagcg tgcacgggtc ggcaacaagc
     5041 cggtgacgaa tcgatcacac tcttgacgca gcgtgccgcc gagcagtgaa atgctcgcga
     5101 ctggcacagg gcccgctccg gcatcatcgg at
//
LOCUS       DATAIW010000142         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_6596, whole genome shotgun sequence.
ACCESSION   DATAIW010000142 DATAIW010000000
VERSION     DATAIW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6596"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VHM48_04590"
     CDS             <1..789
                     /locus_tag="VHM48_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX2754714.1"
                     /translation="DYGAGLQAVDFAGNLDASRVAINRWVAARTSDRIRELLGPDSIN
                     NLTRFVLVNAIFFKASWASPFEPAQTTSATFHRLDGTTLNVPTMHGSFEGSYVRGEGW
                     RAIEIPYVGATMTVILPDAGRFAAVEQALDSDFLAALATRRSFDLVHLAMPRWSSTTS
                     VDLVTTLRQLGVTDLFDPGAADLSGIAAADLHVSQVIHQATIDVDEHGTEAAAATAIA
                     GGTTGGGPVTEVTVTVDRPFFYLIRDGDSGEILFTGRVLDPAAH"
     gene            complement(868..1737)
                     /locus_tag="VHM48_04595"
     CDS             complement(868..1737)
                     /locus_tag="VHM48_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2754715.1"
                     /translation="MRIVVTGGSGKLGRATVSDLLAHGHDVWNVDVAPPREPAGRFTR
                     VDLTDFGQVYELFRGIDEGWESIDGLVHLAAIPGPAQAPNAALFANNIQGSYNVFAAA
                     RHAGIRNIVWASSETLLGLPFDTPPPYVPVDEEYPVRPETAYALAKALDEEMARHFCR
                     WDPALKMIGLRFSNVMEPADYVRFPSFDADPLARKWNLWGYIDARDGAQAVRLALEHP
                     STGMDVFIIANADTVMTRPSAELIAEVFPGVPVRKTLGANETLLSIDKARRILGYEPR
                     HSWRDEVRPGVRQ"
     gene            1871..2191
                     /locus_tag="VHM48_04600"
     CDS             1871..2191
                     /locus_tag="VHM48_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2754716.1"
                     /translation="MQRDANRSPTRDPGLLILASLAAGPRHGYAMVEDIARFGGARLG
                     PGTLYGALARLEEAGLIEPLDSPDRRRPYRLTPAGRDVLATEIDALERVVLTVRSRIA
                     DTSR"
BASE COUNT          339 a          817 c          811 g          334 t
ORIGIN      
        1 gactacggcg cgggcctcca ggcggtcgac ttcgccggca acctcgatgc gagccgggtg
       61 gccatcaacc gctgggtcgc cgctcggacg agcgaccgca tccgggagct gctcgggccg
      121 gacagcatca acaatctcac ccgcttcgtc ctcgtcaacg cgatcttctt caaggcctcg
      181 tgggcgagcc cgttcgaacc ggcgcagacg acctccgcga cgttccaccg cctcgacggc
      241 accacgctca acgtgccgac gatgcacgga tcgttcgagg ggagctacgt ccgcggcgag
      301 ggctggcggg cgatcgagat cccgtacgtc ggggcgacga tgaccgtgat cctgcccgat
      361 gccggccggt ttgcggcagt cgagcaggcc ctcgacagcg acttcctcgc ggcgctcgcg
      421 acgcgccgca gcttcgacct ggtccacctg gcgatgcccc gctggtcgtc gacgacgagc
      481 gtggatctcg tgacgacgct ccgccagctc ggagtcaccg acctgttcga tccgggagcc
      541 gccgacctga gcggcatcgc cgccgccgat ctccacgtga gccaggtcat ccaccaggcg
      601 acgatcgacg tcgacgagca cggaaccgag gcggccgccg cgacggccat cgccggcggc
      661 acgaccggag gcggaccggt cacggaggtg acagtgacgg tcgatcggcc gttcttctac
      721 ctcatccgcg acggcgacag cggcgagatc ctgttcactg ggcgcgtcct ggacccggcg
      781 gcgcactgac cgaccggcgc tcctgacgga ccgggtccgg cggcgcgaaa gggcgtgtca
      841 gccaggaacc ggccatcgtt cgggcgccta ttggcggacg cccggccgga cctcgtcgcg
      901 ccagctgtgg cgcggctcgt agccgagaat ccgccgcgcc ttgtcgatcg agagcagggt
      961 ctcgttcgct ccgagggtct tccggaccgg gacgcccggg aagacctcgg cgatgagctc
     1021 cgccgacgga cgggtcatga cggtgtcggc gttggcgatg atgaacacgt ccatgcccgt
     1081 tgacgggtgc tcgagggcga ggcgaaccgc ctgggccccg tcgcgcgcgt cgatgtagcc
     1141 ccagaggttc cacttccggg cgagcggatc ggcgtcgaac gagggaaatc ggacgtagtc
     1201 ggcgggctcc atcacgttgg agaaccggag gccgatcatc ttgagggccg ggtcccagcg
     1261 gcagaagtgg cgggccatct cctcgtcgag cgccttggcg agcgcgtagg ctgtctccgg
     1321 tcggaccggg tactcctcgt cgacgggaac gtacggcggc ggcgtgtcga acggcagccc
     1381 gaggagggtc tcgctcgagg cccagacgat gttccggatc ccggcatggc gagcggcggc
     1441 aaagacgttg tagctgccct ggatgttgtt ggcgaacagg gccgcgttcg gtgcctgggc
     1501 gggacccggg atcgcggcca ggtggaccag gccgtcgatc gactcccagc cctcgtcgat
     1561 gccccggaac agctcgtaga cctggccgaa gtcggtgaga tcgaccctcg tgaaccgacc
     1621 ggccggctcg cgtggcggag ccacatcgac gttccacacg tcgtggccgt gggcgaggag
     1681 atcgctgacg gtcgcccgcc cgagcttgcc gctgccgccg gtcaccacga tccgcatccg
     1741 tcctccttcg agccggcgat cacgggcatt gccgggcttc atcggaatca tgggcgccgc
     1801 gccgcgccgc gtccagcgcc ccgcacatcg ccggcttgca ttagtacgtc cgacgatcta
     1861 tcgtgggacg atgcaacgag acgccaaccg cagccccacc cgcgaccccg ggctcctgat
     1921 cctcgccagc ctcgccgccg gaccgcgcca cggctacgcg atggtcgagg acatcgcccg
     1981 gttcggcgga gcgcgtctcg ggccggggac cctgtacggc gcgcttgccc ggctcgagga
     2041 ggccggcctg atcgaaccgc tcgactcccc ggaccggcgg cggccgtacc ggctgacgcc
     2101 cgccggcagg gacgtgctgg ccaccgagat cgacgccctc gaacgggtcg tcctcacggt
     2161 ccgcagccgg atcgcggata cgtcgcggtg accgggttcg acggcgacgg cgccggcgcg
     2221 ggtcccggtt ccgaccggac cgccgagccg atccgaccgc gggctcggtg ggttggcgtc
     2281 gcagtgcacc tctttcccga c
//
LOCUS       DATAIW010000143         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211587, whole genome shotgun sequence.
ACCESSION   DATAIW010000143 DATAIW010000000
VERSION     DATAIW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211587"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VHM48_04605"
     CDS             <1..834
                     /locus_tag="VHM48_04605"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX2754717.1"
                     /translation="PDELAIDTGIARRVIGEFIRGQLRQAGFERAVLGLSGGIDSALV
                     AYLVAEAIGADRLLCVLMPYRTSADASRTDAEEVVHRLGSASELVEISPMVDAFFAAD
                     PDATPLRRGNFMARQRMAVLYDRSVTWSGLVVGTGNKTESLIGYTTLFGDNACAFNPI
                     GDLYKSQVRQLATDLGVPDAIIRKAPSADLWPGQTDEGEAGFSYPELDRLLFWRIDKR
                     RSDEEMAGLGFDPALVARVDRMIATSEFKRQVPPIAKLGSRTAGVDYLYPRRRPGSAR
                     E"
     gene            831..1706
                     /gene="rsmI"
                     /locus_tag="VHM48_04610"
     CDS             831..1706
                     /gene="rsmI"
                     /locus_tag="VHM48_04610"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2754718.1"
                     /translation="MSGGRLFVVATPIGNLADVTLRSLEVLGDVPLIAAEDTRLTRRL
                     LDRHGIATRTTSFHAQSAPSRLAELLAHLRGGADLALVTDAGTPSVSDPGDDLVAAWA
                     AEGGVVVPIPGASAVLAVVAASAVAGPRWSFEGFLPRTGRERRVRIARIAADERGTVI
                     YEAPGRVAATLVDLASACGADRPAAIGRELTKLHEEIVRGTLGALADRARSGELTLRG
                     EFALVVGAWTAARIDAESASGTGIADAAAAEMVALAEVERRVAAGSSRRDAARAVAAE
                     TGLPRRRLYRVRGTG"
     gene            1706..2608
                     /locus_tag="VHM48_04615"
     CDS             1706..2608
                     /locus_tag="VHM48_04615"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX2754719.1"
                     /translation="MSGGEREPKIVVVGSTMIDLVAFADRLPGPGETLVGTRFLQGFG
                     GKGANQAVAAARFGASVAMVNAVGDDPNGRAILDNLAAQGVTTDDVAIARGTSGVATI
                     WVDGAGMNRIIVIPGTNALVAPDLAAAAVRRIRPAVVLGQFEIPQATTSAAFLAAREV
                     GATTILNPAPGALIDAALVAVTDWLVPNETEFALIGGRGVAVRPRTGSVAWIPAPTVQ
                     AVDTTGAGDAFVGAFAVGLALGWPAGAAATLGCAVASDSVTRPGTQSSYPDRAAAARF
                     LADVVGTVPVGRPTPSTARQGHDR"
     gene            2605..3732
                     /gene="cax"
                     /locus_tag="VHM48_04620"
     CDS             2605..3732
                     /gene="cax"
                     /locus_tag="VHM48_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2754720.1"
                     /translation="MTEPDSSTRHGIPIIGVLSPIARIVFLIVVGLAVGTIVASLVIA
                     DLSPTLVFGLAATSILGLAWLIGQATQRIGAITGPQVGGILNATFGNIAELIIAFFAL
                     QAGLIDVVKASLTGSIIGNLLLVLGLSVLVGGLRNGRQTFSREIAGSNAALLVLAAIG
                     LFVPAVLALSSGGGEGSVVEDSVLVAVFLIAGYVLSLIYQFTNPSGTLGGHEARDTHE
                     GPGWSGRTAAVVLVGAAILLAILSEILVGAIQPVVDTLGLTAFFVGVIILPVIGNLAE
                     QLVAVQLAFKNRMEFSMAVSYGASLQVALFVAPVLVLLGVLTGHPMDLVFAPLEIVAV
                     GAAVGISALIALDGESNWLEGALLVIVYLILAVSFFELVPK"
     gene            complement(3802..4269)
                     /locus_tag="VHM48_04625"
     CDS             complement(3802..4269)
                     /locus_tag="VHM48_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2754721.1"
                     /translation="MNRVLLTGRLTRDPEMRSLASGKAVTQFSVATNEYIGQGKEKAE
                     YHNIVTWDRLAQTCGEFLGKGQQVAIEGRIQTRTWDDDSGKRHWKTEIVANHVEMLSG
                     RRKKDYAAESAANGLAAQAVAYGDEDEADDGEAAAIAAAAEPLTEADEEVVAA"
BASE COUNT          555 a         1617 c         1632 g          722 t
ORIGIN      
        1 cccgacgagc tggcgatcga caccggcatc gcccgccggg tcatcggcga gttcatccgc
       61 ggccagctcc gccaggccgg cttcgagcgg gcggtgctcg gcctgtcggg cgggatcgac
      121 tcggcgctcg tcgcatacct cgtcgccgag gcgatcgggg ccgatcgcct gctgtgcgtc
      181 ctgatgccgt accggacgtc ggcggacgcg tcacggaccg acgccgagga ggtcgtccac
      241 cggctcggca gcgcgagcga gctggtcgag atcagcccga tggtcgacgc cttcttcgcc
      301 gcggaccccg acgcgacgcc gctccgccgg ggcaacttca tggcccgcca gcgaatggcc
      361 gtcctgtacg accgatcggt gacctggagc gggctcgtcg tgggcaccgg caacaagacg
      421 gagtcgctga tcggctacac gaccctcttc ggcgacaacg cctgtgcctt caatccgatc
      481 ggcgacctct acaagagcca ggtccgccag ctggcgacgg acctcggcgt gcccgatgcg
      541 atcatccgca aggctccctc ggccgacctc tggccgggcc agaccgacga aggggaggcg
      601 ggattctcgt acccggagct ggaccggctg ctgttctggc ggatcgacaa gcgccggtct
      661 gacgaggaga tggcggggct gggcttcgat ccggcgctcg tcgcccgggt cgaccggatg
      721 atcgcgacct ccgagttcaa gcggcaggtc ccgccgatcg ccaagctcgg ttcgcggaca
      781 gcgggcgtgg actacctgta cccgcgacgc cgcccgggct ccgcacgcga gtgagcggcg
      841 ggcgcctgtt cgtcgtcgcg acaccgatcg ggaacctcgc cgacgtcacc ctccgctcgc
      901 tcgaggtcct cggtgacgtc ccgctgatcg cggccgagga cacccggctg acgcgacgtc
      961 tgctggatcg ccacgggatc gcgacccgga cgacgagctt ccacgcccag agcgcaccga
     1021 gccggctggc cgagctgctc gcccatctcc gcggcggggc ggacctcgcc ctcgtgaccg
     1081 acgcgggtac gccgtccgtg agcgaccccg gcgacgatct ggtcgcggcc tgggcggcgg
     1141 agggcggagt ggtcgtcccg atcccgggcg cgtcggccgt gctggcggtg gtcgcggcgt
     1201 ccgcggtggc ggggccgcgc tggtcgttcg aggggttcct gccgcggacc gggcgcgagc
     1261 ggcgggtgcg gatcgcccgg atcgcggcgg acgagcgggg gacggtgatc tacgaggcac
     1321 ccggccgggt cgcggccacg ctcgtcgacc tggcatcggc ctgcggcgcg gaccgccctg
     1381 cggcgatcgg gcgggagctc acgaagctcc acgaggagat cgtgcggggc acgctcggcg
     1441 cgctggcgga ccgggcacgg agcggcgagc tgacgctccg cggcgagttc gccctcgtgg
     1501 tcggcgcctg gaccgccgcc cggatcgatg ccgaatcggc ctccgggacg gggatcgccg
     1561 atgcagcggc cgccgagatg gtggcactcg cggaggtcga gcggcgggtc gcggccggct
     1621 cctcgcgccg cgatgccgcc cgggcagtcg ccgccgagac cgggctcccg cgacggcggc
     1681 tgtaccgcgt ccgcgggacg ggctgatgag cggtggcgag cgggagccga agatcgtcgt
     1741 cgtcggcagc acgatgatcg atctcgtcgc gttcgccgat cgccttccgg gacctgggga
     1801 aacgctcgtc gggacccgct tcctgcaggg gttcggcggc aagggcgcga accaggcggt
     1861 ggccgcggca cggttcggag cgtccgtggc gatggtcaac gccgtcggcg acgaccccaa
     1921 cggccgggcg atcctcgaca atcttgccgc gcagggcgtg acgaccgacg atgtcgcgat
     1981 cgcgcgcggg acctccgggg ttgccacgat ctgggtcgac ggcgcgggga tgaaccggat
     2041 catcgtcatc cccggcacga acgccctcgt cgcgccggat ctggctgcgg ccgccgtgcg
     2101 gcggatccga ccggcggtcg tcctcggcca gttcgagatc ccgcaggcca cgacgagcgc
     2161 ggccttcctc gccgcccgcg aggtgggagc gacgacgatc ctgaatccgg cacccggcgc
     2221 cctgatcgat gctgcgctgg tcgccgtcac cgactggctg gtgccgaacg agacggagtt
     2281 cgccctgatc ggcgggcgcg gggtggccgt ccggccgcgt accggatcag tcgcctggat
     2341 cccggccccg acggtccagg ccgtcgacac gaccggggcc ggcgacgcgt tcgtcggtgc
     2401 gttcgcggtc ggcctcgcgc tcggctggcc ggccggtgcc gccgcgacgc tcgggtgcgc
     2461 cgtggcctcc gacagcgtga cccggcccgg cacgcagagc tcgtatcctg atcgcgcggc
     2521 ggcggcccgg ttccttgccg atgttgtcgg gacggtgccc gtcggccgac cgacgccatc
     2581 caccgcccgc cagggacacg acagatgacc gagcccgatt cgtccacccg acacggcatc
     2641 ccgatcatcg gcgtcctctc gccgatcgcg aggatcgtct tcctgatcgt cgtcggactc
     2701 gcggtcggga cgatcgtcgc ctcgctcgtc atcgcggacc tgtcaccgac cctcgtgttc
     2761 ggcctcgccg ccacctcgat cctcggcctc gcctggctga tcggccaggc aacccagcgg
     2821 atcggggcga tcaccggtcc ccaggtcggc ggcatcctca acgcgacgtt cggcaacatc
     2881 gccgagctga tcatcgcgtt cttcgcgctc caggccggcc tgatcgacgt ggtcaaggcg
     2941 agcctgaccg gttcgatcat cggcaacctc ctgctcgtcc tcgggctgag cgtgctggtc
     3001 ggcggcctga ggaacggccg ccagaccttc tcgcgggaga tcgccgggtc gaacgccgcg
     3061 cttctcgtcc tggccgcgat cggcctgttc gtgccggcgg tcctcgctct gagcagtggt
     3121 ggcggcgagg gcagtgtcgt cgaggattcg gtgctcgtcg ccgtgttcct gatcgcgggc
     3181 tacgtgctct cgctgatcta ccagttcacc aacccgtccg ggacgctcgg cggccacgag
     3241 gcgcgcgaca cccacgaggg ccccggctgg tccggccgga ccgccgcggt cgtcctggtc
     3301 ggcgcggcga tcctgctcgc gatcctgtcg gagatcctcg ttggggcgat ccagccggtc
     3361 gtcgacacgc tcggactgac cgccttcttc gttggcgtga tcatcctgcc ggtcatcggc
     3421 aacctcgccg agcagctcgt cgcggtccag ctcgcattca agaaccggat ggagttctcg
     3481 atggccgtgt cgtacggcgc gagcctccag gtggcgctat tcgtcgcgcc ggtgctggtc
     3541 ctcctcggcg tcctgaccgg ccacccgatg gatctcgtct tcgcgccgct cgagatcgtc
     3601 gccgtcggtg ccgcggtcgg gatcagcgcc ctgatcgcgc tcgacggcga atcgaactgg
     3661 ctcgagggcg ctctcctcgt catcgtctac ttgatcctcg ccgtgtcctt cttcgagctc
     3721 gtcccgaagt agtgggggcc ggcggcggga gggggagccg ccgccggccc ggcgaacaat
     3781 cggtgcgtgc cgcgtggacg ttcaggcggc gacgacctcc tcgtccgctt cggtgagtgg
     3841 ctctgctgcc gcggcgatcg cggccgcctc gccgtcgtcg gcctcgtcct cgtcgccgta
     3901 ggccacggcc tgcgccgcga ggccgttcgc cgccgactcg gccgcgtaat ccttcttgcg
     3961 gcggcccgac agcatctcga cgtggtttgc gacgatctcc gtcttccagt ggcgcttgcc
     4021 gctgtcgtcg tcccaggtcc gggtctggat ccggccttcg atcgcgacct gctggccctt
     4081 gccgaggaac tcgccgcagg tctgggccag gcggtcccat gtgacgatgt tgtggtactc
     4141 ggccttctcc ttgccctggc cgatgtactc gttggtggcg acgctgaact gcgtcaccgc
     4201 cttgccgctc gcaaggctgc gcatctccgg gtcgcgcgtg aggcgaccgg tcaagaggac
     4261 ccgattcacg gtccgttctc cttcttggcc gcccgacgat cagtcggcgg cctgcgttcc
     4321 ggcgggaaca gcacgacggg tggcgggacg atggaagagg cgccggcccc ggcggtcggg
     4381 ccaatccggc gggtcgtgga ccatcggtgg tcggcggacc gtggacggtg tcgctcggcg
     4441 ggaccggctg ttgcctggcg ggcagagaga acctaacagc cggccctgca ccggcgctta
     4501 tcggctactt cctgatgggt tacccg
//
LOCUS       DATAIW010000144         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_66966, whole genome shotgun sequence.
ACCESSION   DATAIW010000144 DATAIW010000000
VERSION     DATAIW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66966"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1078
                     /locus_tag="VHM48_04630"
     CDS             74..1078
                     /locus_tag="VHM48_04630"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2754722.1"
                     /translation="MTTLEQTGEKVLANVERVIVGKHHEVRLALVALLCRGHLLIEDV
                     PGTGKTVLAKAIARSLGCSFRRIQFTPDLLPSDVTGLSIYNQKTQEFEFRPGPIMSQV
                     VLADEINRATPKTQSSLLESMEERQATVDGTTYAMPDPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRIRLGYPQPLEEVVILDEQKRHHPLEELEVVCSVDELRGMQNAIRDIYVD
                     PSVAEYIVRLVGATRVHPDVYLGASPRGSIALYRAGQALAGLMARDYVIPDDIKALAE
                     PALAHRLIIKTSSSIHDVQSGSVVHELLDSIPIDGIRPTGGPNGPRAIKPTQQSAASA
                     "
     gene            1116..>2090
                     /locus_tag="VHM48_04635"
     CDS             1116..>2090
                     /locus_tag="VHM48_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2754723.1"
                     /translation="MIRRFQLIVIAAILLIAAFSTELEFLFYLVYLSILVIGGSYILT
                     RLGLADLEAGYAVSQLTGHVGDRLQITYTLRNTSRVPKPWLEVHNPTTLPGGLPGRAI
                     SLGSRAERSWLVRAPLIRRGHFRVEPLQIRTGDPFGFFEASASVGQGVAVVVYPRIEK
                     LPLWRLPAASIEGSHAAPERTLQTSPLATAVRPYAPGDAFNRIHWKSTARHGEIQVKE
                     FELEQTSDAWIFVDLERAVQGGRGDESTVEVAVRAAASIAAKALLENRAVGLTVNGHR
                     QAMVPVDRGSRQHLKIMQLLAAVDGDGTTPLVECLVNSAGRLRRGMTAI"
BASE COUNT          331 a          775 c          668 g          316 t
ORIGIN      
        1 cgcctgccgg acgtactatc gtgcaaaccc cccatcgccg gacgctcagt ccgcgattcc
       61 tgcgaggagc ctgatgacga cccttgagca gaccggagag aaggtcctcg ccaacgtcga
      121 gcgagtgatc gtcggcaagc accacgaggt tcgcctcgct ctcgtcgctc tcctctgccg
      181 cggccatctc ctgatcgagg acgtgccggg gaccggcaag acggtcctcg ccaaggcgat
      241 cgcccggagc ctcggctgca gcttccggcg catccagttc acgccggacc tcctgccgtc
      301 ggacgtgacg ggcctctcga tctacaacca gaagacccag gagttcgagt tccggcccgg
      361 cccgatcatg agccaggtcg tcctcgccga cgagatcaac cgggcgacgc cgaagaccca
      421 gtcgagcctc ctcgaatcga tggaggagcg ccaggcgacc gtcgacggca cgacctacgc
      481 gatgcccgac ccgttcctcg tgatcgcgac ccagaacccg atcgagtacg agggaacatt
      541 cgcccttccg gaggcccagc tcgaccggtt catgctccgg atccgcctcg gctatcccca
      601 gcccctcgag gaagtcgtga tcctcgacga gcagaagcgc caccatccgc tcgaggagct
      661 cgaggtcgtg tgctcggtcg acgagctgcg cggcatgcag aacgcgatcc gtgacatcta
      721 tgtcgacccg tcggtcgcgg agtacatcgt ccgcctcgtc ggcgcgaccc gggtccatcc
      781 cgacgtctac ctcggcgctt ctccgcgcgg atcgatcgcc ctgtatcggg cgggccaggc
      841 cctcgccggg ctgatggccc gcgactacgt gatcccggac gacatcaagg cgcttgccga
      901 gccggccctc gcccatcggc tgatcatcaa gaccagctcg tcgatccacg acgtccagtc
      961 cggcagcgtc gtgcacgagc ttctggactc gatcccgatc gacggaatcc ggccgaccgg
     1021 cggcccgaac ggaccgcgcg cgatcaagcc gacccagcag agcgccgcga gcgcctgagc
     1081 agccacgaac cgacccgacg agtgacccga ccgcgatgat ccgtcgcttc cagctcatcg
     1141 tcattgccgc gatcctcctg atcgcggcgt tctcgaccga gctggagttc ctcttctacc
     1201 tcgtctacct ctcgatcctc gtcatcggcg gctcgtacat cctgacccgg ctcggcctgg
     1261 ccgacctcga ggcgggctac gcggtcagtc agctgaccgg tcacgtcggc gatcggctcc
     1321 agatcacgta caccctgcgc aacacgagcc gggtgcccaa gccgtggctc gaggtccaca
     1381 acccgacgac gctgcccggc ggcctgccgg gccgggcgat ctcgctcggc tcccgagccg
     1441 agcggtcgtg gctggtccgg gcgcccctca tccgccgcgg ccatttccgg gtcgagccgc
     1501 tccagatccg gacgggcgac ccattcggct tcttcgaggc atcggcctcg gtcggccagg
     1561 gcgtcgcggt cgtggtctat ccgcggatcg agaagctgcc cctgtggcgg ctgccggcgg
     1621 cgagcatcga ggggagccac gcggcacccg agcggacgct ccagacctcg cccctggcga
     1681 cggccgtccg gccgtacgcg cccggtgacg cgttcaaccg gatccactgg aagtcgacgg
     1741 cccgccacgg cgagatccag gtcaaggagt tcgagctcga gcagacgtct gacgcgtgga
     1801 tcttcgtcga cctcgagcgg gcggtccagg gcgggcgcgg cgacgagtcg accgtcgagg
     1861 ttgccgtccg cgctgccgcg tcgatcgcgg cgaaggccct cctcgagaac cgggcggtcg
     1921 gcctgaccgt caacggacac cgccaggcca tggtgccggt cgaccggggc agccgccagc
     1981 acctcaagat catgcagctc ctggctgccg tcgacggcga cggcacgacc ccgctggtcg
     2041 aatgcctggt caactcggcg ggtcgcctgc gccgggggat gaccgcgatc
//
LOCUS       DATAIW010000145         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211660, whole genome shotgun sequence.
ACCESSION   DATAIW010000145 DATAIW010000000
VERSION     DATAIW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211660"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHM48_04640"
     CDS             complement(<1..536)
                     /locus_tag="VHM48_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2754724.1"
                     /translation="MNADNLTILVALAAGLLSFLSPCVLPLVPAYIGQLTAVAVADAS
                     RAGTLSADGSPTRGAGPSRWTAFRHALAYVGGFGAVFTLLGVTATFFAAGFSPWMPIL
                     RQVGGVILVLLGLNLAGVLRIPALDRNWRPLDAGASASVATTTGSMSFAPAGGPGFGD
                     RLGGRIVSSRGGWLASFGL"
     gene            complement(556..840)
                     /locus_tag="VHM48_04645"
     CDS             complement(556..840)
                     /locus_tag="VHM48_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX2754725.1"
                     /translation="MTTPLPELVLYTRDGCHLCEEARAIVQGLLEDRAARGQRTAAIH
                     ERDITTDPEWERQFFATIPVVEIAGRRLELATSAAKLRRFLDDVLDGSLV"
     gene            1005..>2353
                     /locus_tag="VHM48_04650"
     CDS             1005..>2353
                     /locus_tag="VHM48_04650"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX2754726.1"
                     /translation="MAVFEQTMDRERSRRVPRPGRRFFLFVLTTILGVAAVVGALAVY
                     HERQAGRILPGVSAAGVDVSGMTIDEARMALESNLAGLSTGAVTIRSSLGSTTIAFAD
                     VARVPDIAGMVADAVARGRGGSWLDETIAGIRLELRPETVPLRLGYDHDRAATAVGAF
                     VDRMTLNAIDASVAGGAAGFGVIASVDGQRIDPATTLSAIDRAMRDPATRAGAVIPAP
                     VERVAPTLTTEGARAAKAVAGRVTAALTVATGTRTWEIRPWRIRPWISFAWVDGEYRP
                     VIDRTKIPAVLKTIAKAVARPVRDAAFLRDKHGRIVGSMADRAGRKLDVVVSTNAIAA
                     ALEARIEIATATPPIKLAIATIAPKRTTEEAAKTAPLMVKLGSWTTYYQVAAHNGFGA
                     NITVPTRVLDGTVVAPGAVFDFWAGLGEVSLRTGYKLGGAIVGGHSVEGKALAGGICA
                     "
BASE COUNT          360 a          824 c          847 g          322 t
ORIGIN      
        1 agcccgaagc tggcgagcca gccgccgcgg ctgctgacga tccggccacc gagccggtcg
       61 ccgaagcccg gtccaccggc cggagcgaag gacatcgagc ccgtcgtcgt cgcgaccgag
      121 gcggacgccc cggcatcgag cggtcgccag ttgcgatcga gtgccgggat ccggagcacc
      181 ccggcgaggt tcagcccgag gaggacgagg atcacgccgc cgacctggcg gaggatcggc
      241 atccacggcg agaacccggc ggcgaagaac gtcgcggtca ccccgagcag ggtgaatacc
      301 gccccgaacc cgccgacgta ggcgagagcg tggcggaacg ccgtccagcg cgatgggccg
      361 gctccacggg tcggcgagcc gtcggcagag agtgtgccgg ctcggctcgc atccgcgacg
      421 gccaccgcgg tcagctggcc gatgtaggca ggcacgagcg gcaggacgca cggactgagg
      481 aagctcagga gaccggccgc gagggcgacg aggatcgtga gattgtccgc gttcatcgag
      541 tgcgcccgtg accgatcaca cgaggcttcc gtcgaggacg tcgtcgagga accgccgcag
      601 cttcgcggcc gacgtggcca gctcgaggcg gcggcccgcg atctcgacga ccgggatcgt
      661 tgcaaagaac tgacgctccc attctgggtc ggtcgtgatg tcgcgctcgt ggatcgccgc
      721 ggttcgctgc ccgcgcgcgg cacgatcctc gagcaggccc tggacgatgg cgcgcgcctc
      781 ctcgcagagg tggcacccgt cgcgggtgta gaggacgagc tcggggaggg gagtggtcat
      841 tgcagcctgc attctagggt cgggagcgcc acaccccggt tgggcgcccc gccccaggag
      901 ccgacgggta acgaaccacc cggactggac gtcgaagcag ttgcacccga tgatgcgtgc
      961 cgtcgacggg cgtacccgcc ggtcagcgcg aggggtttgg ggcgatggcg gtcttcgagc
     1021 aaacaatgga tcgcgaacgt tcccggcgcg ttccgcgacc cgggcgccgg ttctttctgt
     1081 tcgtcctcac gaccatcctg ggtgtcgcgg ccgtggtcgg cgcgctggct gtctaccacg
     1141 agcgtcaggc cggccggatc ttgccgggcg tctccgcggc cggtgtggac gtcagcggca
     1201 tgacgatcga cgaggcccgc atggccctcg agtccaacct ggcgggtctc tcgaccggcg
     1261 ccgtcacgat ccgctcgagc ctgggctcga ccacgatcgc gttcgctgat gtcgctcgcg
     1321 ttcccgacat cgccggcatg gtcgccgacg ccgtcgcccg aggacgaggc ggctcctggc
     1381 tcgacgagac gatcgccggc atccgtctcg agctccgacc cgagaccgtg ccactgcgtt
     1441 tgggctacga ccacgatcgg gccgcgaccg ccgtcggcgc cttcgtcgat cggatgaccc
     1501 tcaacgccat cgacgcgtcg gtcgcgggcg gagcggccgg cttcggcgtg atcgcatcgg
     1561 tcgacggaca gcggatcgat ccggcaacga cgctctcagc gatcgatcgg gcgatgcgcg
     1621 acccggcaac acgtgccggt gctgtgatcc cggcgcctgt cgagcgagtc gccccgacac
     1681 tcacgaccga gggcgcgcgg gcggcaaagg cggttgccgg ccgcgtgacc gccgccctga
     1741 ccgttgcgac cggcacgagg acgtgggaga tccgaccgtg gcggatccga ccgtggatct
     1801 ccttcgcctg ggtcgacggc gagtaccgac ccgtgatcga tcggacgaag attccggcgg
     1861 tcctcaagac gatcgccaag gccgttgccc ggccggtccg cgatgccgcg ttccttcgcg
     1921 acaagcacgg ccggatcgtc ggctcgatgg cggatcgggc cggccggaag ctcgacgtcg
     1981 tggtcagcac caacgccatc gccgccgccc tcgaggcgcg catcgagatc gccaccgcca
     2041 cgccgccgat caagctcgcg atcgcgacga tcgctccgaa gcggacgacc gaggaggcgg
     2101 ccaagaccgc cccgctgatg gtcaagctcg gcagctggac gacctactac caggtcgccg
     2161 cccacaacgg cttcggtgcg aacatcacgg tcccgacgcg ggtcctcgac ggcacggtcg
     2221 tcgcccccgg cgccgtcttc gacttctggg ccgggctcgg cgaggtcagc ctgcggaccg
     2281 gctacaagct cggcggcgcg atcgtcggcg ggcactcggt cgagggcaag gcgctcgccg
     2341 gcgggatctg cgc
//
LOCUS       DATAIW010000146         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_96791, whole genome shotgun sequence.
ACCESSION   DATAIW010000146 DATAIW010000000
VERSION     DATAIW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96791"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..528)
                     /locus_tag="VHM48_04655"
     CDS             complement(232..528)
                     /locus_tag="VHM48_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754727.1"
                     /translation="MTRPTERPLERPNVPARTRLLTVVAAYLALTGAMAIIYAGFVPI
                     PPFSDVPAPLVGQVLGAIALVAAIAVFVSRRGAGRPGCSSSPSASGSPVFASHP"
     gene            complement(1048..1698)
                     /gene="folK"
                     /locus_tag="VHM48_04660"
     CDS             complement(1048..1698)
                     /gene="folK"
                     /locus_tag="VHM48_04660"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2754728.1"
                     /translation="MECREVGRLDLRRVRAYIGLGANLGDADGALARGIRGLAALPGA
                     RLRAVSRLYATEPVGVVDQPPFRNAVAALDVPAGRDAATGATALVIALKQLERAFGRQ
                     DRERWGPRELDLDLLVFGRALLSIDRPPKGLSLDPGKADLLLVVPHRDAADRLFVLAP
                     LADLAAGLVPPGWPETVASAAARRRIVEGAAAARPIAAWDPATSAWAAISPRPTSG"
     gene            1702..>2436
                     /locus_tag="VHM48_04665"
     CDS             1702..>2436
                     /locus_tag="VHM48_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754729.1"
                     /translation="MIDVAVLILHPTPAGRAGTIEAWVTAGRAAIAERHRVGFLEAGA
                     NEVTVVSGPPDRASFGERLRGFVTARRPRGVVVLGSGAMPLATRSDRRAFVEAAAASG
                     RNALANNRFSADVVAIATAATLDELPDLATDNALPRWLADVARYEVSDLRRRWRPGFD
                     IDGPLDLLLLGPRGWLPAPPDGIVDGVAERLAMVRAVARDTRAELVVTGRLSAVGLAW
                     LERATASRTRALIEERGFRTRRDGQRP"
BASE COUNT          353 a          873 c          889 g          321 t
ORIGIN      
        1 ctcctggggt ggccccacgg agagcctcag gtaaagcggc acgagcagga acgccgcgag
       61 cgcgaccgca tacgcgagga gtgcccccgg caggcggccc gggcgatcgg atccgtgatg
      121 aagatcaggc acgcgatggc cagcgctgaa cgagcaccca caggctgaac aagctgagca
      181 ccatgtcaac ggcgagggcc aacacggtga agatcggact tgtctcggag gctacggatg
      241 cgacgcgaag accggcgagc ccgaggccga cggcgacgac gagcaccccg gccgtccggc
      301 cccacgcctg ctgacgaaga ccgcgatggc ggccaccagc gcgatcgcgc ccagcacctg
      361 tcccacgagt ggggccggga cgtcgctgaa cggcgggatc gggacgaacc ctgcgtagat
      421 gatggccatc gccccggtga gcgcgaggta cgccgcaacg accgtcagga gtcgcgttcg
      481 ggcggggacg ttcggcctct cgagcggtcg ttcggtcgga cgagtcatcg tcgccctcct
      541 cgccggctcc gagtcaggtg ttgatgagag tggagatgcc gagccgactt caagcgattg
      601 gttcgcctac attccgccta tctgtcctcc tcgggatgcc gatcgttcgg cgggatcggg
      661 ccgatacccg cgtacgggtt tggctcgtcc gactgctccc gccaacggag cggcccatga
      721 ccagcgtcgg acagctgttc cgcgagcgac ccgactgcgt gccacaggcg cccgacaccc
      781 gcgaggagag cggccggaaa ccccctgatg ccccggcccg gatgcggtga tacgtctccc
      841 gccgatgaac ccgtcatgtc aagtcctccc gcagagctct tgaggcgcct tcggcctcag
      901 tcaccgctgt ctctgaaaac gttgcctcgg cctgtctcgt tgcaccagag gcgaacggcc
      961 cgccggacgt ccggcgcgtg ggccgatgag tctccgaagc gcagtggatg acatgcaacg
     1021 actgggtcat cgatccgcct ggatgggtca gcccgacgtc ggcctcggac tgatcgccgc
     1081 ccaggccgat gttgccggat cccacgcggc gatcggtcgg gcggcggctg caccctcgac
     1141 gatccgtcgg cgagccgccg cgctcgccac cgtctcgggc cagcccggcg gcacgagacc
     1201 cgccgcgaga tcggccaggg gggcgaggac gaacaggcgg tccgcggcgt cgcgatgggg
     1261 gacgacgagc agcagatccg ccttgccggg atccagggag agccccttcg gcggccgatc
     1321 gatcgacagc agggcacggc cgaagacgag caggtcgagg tcgagctcgc gcggccccca
     1381 gcgctcccga tcctggcgcc cgaacgcgcg ctcgagctgc ttcagcgcga tcacgagtgc
     1441 cgtcgcgccg gtcgcggcgt cccgaccggc gggcacgtcg agggcggcga ccgcgttgcg
     1501 gaacggcggc tggtcgacga cgccgaccgg ctccgtcgcg tacagccgcg agacggcgcg
     1561 gaggcgggcg cccggcagcg ccgccaggcc gcggatgccg cgggcgagcg ccccgtccgc
     1621 gtcgccgagg ttcgccccga ggccgatgta cgcccggacg cgtctcaggt cgagcctccc
     1681 gacttcccta cactccaccc cgtgatcgat gtggccgtgc tcatcctgca cccgacaccc
     1741 gccgggcgcg cgggaacgat tgaagcgtgg gtcacggctg ggcgagccgc gatcgccgag
     1801 cgccaccgcg tcgggttcct tgaggccggt gcgaacgagg tgaccgtcgt gtccggcccg
     1861 ccggacaggg cgtccttcgg ggagcggttg cgcgggttcg tcaccgcccg gcgaccgcgg
     1921 ggcgtggtcg tgctcgggtc cggcgcgatg cccctcgcaa cgagatcgga ccggcgggcg
     1981 ttcgtcgagg cggccgcggc atcggggcgc aacgcgcttg ccaacaaccg gttctcggcc
     2041 gacgtcgtcg cgatcgccac cgccgcgacc ctggacgagc tcccggatct ggccaccgac
     2101 aacgcccttc cgcgctggtt ggccgacgtc gcccgatacg aggtctccga cctgcggcgg
     2161 cgctggcggc ccgggttcga catcgacggg ccgctcgatc tgctcctcct cgggccgcgg
     2221 gggtggctgc cggctccgcc cgacgggatc gtggacgggg tggcggagcg gctcgccatg
     2281 gtccgggccg tcgcccgcga cacccgggcg gagctcgtgg tgaccggtcg cctgtccgcg
     2341 gtcggcctcg cctggctgga gcgggcgacc gcatcccgaa cccgggccct gatcgaggag
     2401 cgtgggttcc ggacccggcg ggacggccag cggccg
//
LOCUS       DATAIW010000147         8523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_271421, whole genome shotgun sequence.
ACCESSION   DATAIW010000147 DATAIW010000000
VERSION     DATAIW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271421"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VHM48_04670"
     CDS             complement(<1..1397)
                     /locus_tag="VHM48_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2754730.1"
                     /translation="MNNPPEGDAAVTPRRGSRHLHAFLDSLLPGLGHLAAGRRRRAAL
                     FGIPTLVTIIVVVGGLLLIPTSRLLGIALDPATVVVLFGLQALLLVWRAAAIGSSLVD
                     GRFPRLGRRDALPIALLAVVLVAPHGFAAYATEVAREATDQIIPIDPTTTGAWDPSAS
                     ASAVPDPEVTLPSEVPASPSVPSSASPSPAVPRQNVLLVGVDAGVGRNTYLTDTMIVV
                     SLDPVGQTVSMISIPRDMVDVPLPDGRTFSGKINGLVSFARHHQGTFPGSNGKGYDVL
                     MGALGTLLGLRIDHYAAVNLQGFVNVVDTLGGIDVDVADGFCDPTYDEYGFTHGFGIT
                     AGRHHLNGQQALGYARIRKAAGESDFTRAARQQEILSGIRDRVKSGGFLNDPIGLLRA
                     LSDTVETNIPRKLVPDLADIATKIGRKQTYRAVITHPLVKGDYDGRGSIQVPDVAAIR
                     KLVAAMFPATGTPPP"
     gene            complement(1482..1904)
                     /locus_tag="VHM48_04675"
     CDS             complement(1482..1904)
                     /locus_tag="VHM48_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2754731.1"
                     /translation="MVIQDAEAAPAEILALVQSAYRAFAARDINALLGLLHEDVIWGE
                     APNPLIPSAGMRFGVDGVLEWLRIGKETETILAFEPVRFLVDGDTVAVIGWTTIEARP
                     TGKVYDTDFVHVVTVADGRIVRFQEFFDTWAAAEAFRA"
     gene            complement(2176..4461)
                     /locus_tag="VHM48_04680"
     CDS             complement(2176..4461)
                     /locus_tag="VHM48_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2754732.1"
                     /translation="MSRFFTGLGRFAVRFRFLIVVVWVVVTVLAVQLLPSLGDVAKDT
                     TSGFLPADSPSMQAAAMAAPFQDTSLAAATVVMARDGGLTAADNASMDALETQIRGID
                     RVKVVVDLGVSSDGQARQALVEAAVVAFSAGPEAKGVVDAIRATIKRSVPPIGLEIHV
                     TGQLATQVDTVAASGSSQNLTQQLSLLFIVVLLVLAFRAVLAPIVTLVPAAFVLILSG
                     PVIAESTKIGVQVSSITELLLIVLILGAGTDYGVFLVFRVREELRRGLTAHEAVIQSV
                     SRVGESITFSALTVMAALLSLAIAQFAFYQSLGPALAIGIGLMLVAGLTLLPALLAIF
                     GRAVFWPTGATARAHDRHGMWDRIGIMATRRPGPTLAAGVVLFAALGATLLTTGVAGF
                     GGAATSPSGTDSAAGSASIEQHFPSSNASRSAVLMTFATPVWSDLSSVSRAQDGLTGL
                     TEFGGLVGPLNPIGIAITTDQLAELHTNLGPARELPPVPATADVSPQLYAAYRATGQL
                     ISPDGRTIQFSVEFASGDLSSPAALDSVPAMRADVERVAMAAGATASGVYGQVAFASD
                     VSHISGADLVRIIPIVAVLIALLLAIVLRSLVAPLYLVASIVLSYLAALGLVGIVFVH
                     LGGQDGINFLLPFLMFVFLMALGSDYNILIMSRIREEAHDLPLRDAVARAIGRTGSTI
                     TTAGVILGGTFAVLAVAGGSAGGGQIQQIGYGIAAGVLMDTFLLRSLLVPSLVVLLGR
                     RNWWPSHLAMVSPAGDPPPTT"
     gene            4552..4779
                     /locus_tag="VHM48_04685"
     CDS             4552..4779
                     /locus_tag="VHM48_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754733.1"
                     /translation="MTKRPKATLGGQHRKDKIGGDVPAFAGPPGVERHGEEHQDDAAG
                     RHNRGSSSDPIARIGVRPIAPSQVVPGLVTD"
     gene            complement(4917..5351)
                     /locus_tag="VHM48_04690"
     CDS             complement(4917..5351)
                     /locus_tag="VHM48_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754734.1"
                     /translation="MQSALAAVGLPEAPGAGPARSSYEPGVCNIGPAEIARRRTAGRV
                     GLAVTVILLATLVAVGAPPLTRLVLVLPAAGAASGYLQAWLRFCAGFGSRGVFNFGVP
                     GQTEGVADPVDRAADRARATQIGVASLVVGVVVGVAAALLPL"
     gene            5489..5947
                     /locus_tag="VHM48_04695"
     CDS             5489..5947
                     /locus_tag="VHM48_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX2754735.1"
                     /translation="MDEADDREELARRGWRLLMGAGQRHLEVVTAGIEEMGLSKVMAQ
                     FLGAVCQSPPGPTNQLATRFGVDPGWVTDIVDRLEARGQLVRRQSPNDRRVKILDVTE
                     AGRETFRAMEAIFATPPPELLDASREDLLALVRIAERLAGPIEPVDAVDP"
     gene            complement(6023..6697)
                     /locus_tag="VHM48_04700"
     CDS             complement(6023..6697)
                     /locus_tag="VHM48_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX2754736.1"
                     /translation="MATRTNSTAEPRSRLSRERVVRAAIAHADAGGLEALSMRTLAEE
                     LGVAPMALYRHVANKDDLVDGMVDVVVSEVDLPSSGADWRTAMRRRAISLRDVLSRHR
                     WAIGLMESRVNPGPASLRHHDAVIGCLRAAGFDMELVAHAYSLLDGYIYGFALTKMNL
                     PFESGDNVAEVAQTMLEPFPMNEYPNLVAFITEHVMKPGYDFGDEFEYGLDLLLDGLE
                     RTRNGA"
     gene            6843..7802
                     /locus_tag="VHM48_04705"
     CDS             6843..7802
                     /locus_tag="VHM48_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2754737.1"
                     /translation="MQAIVQDSYGSHEALKVGDVDKPEIGADEVLVRVQAASIHVGDW
                     ILMTGSPFVMRLATGLRKPKNRVPGTDVAGTVEAVGSRVQRLAVGDDVFGWCAGAFAE
                     FARASEDQFVKKPANLTFEQAAAVGVSATTALQLLRDDGRIRSGQKVLINGASGGVGT
                     FAVQIAKASGAEVTGVCSTKNVDMVRSIGADHVIDYTREDFTKGTERYDLILDNVGNH
                     SMARTRRVLTPLGTLISNGGGHSDGKLGRTVRTMLVSMFVRQQASPTVKTQNREDLVA
                     LKGLVEGGKVTPVIDRTYPLTETPEAVGHVGAGHARGTVVITV"
     gene            7861..8268
                     /locus_tag="VHM48_04710"
     CDS             7861..8268
                     /locus_tag="VHM48_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754738.1"
                     /translation="MSKATVTRLFIGSLIAFAGGAILAVAAVWLAMANDVFVMAGPDI
                     VGLRGSAFAWSLLGLGIVGGVAMGGALIGGLVSWIGALLNTWQLESRTWFAVLLLLGI
                     FNVGFLAMIAYLIAGPDSTAAVTPRGAHAPVAA"
     gene            8268..>8523
                     /locus_tag="VHM48_04715"
     CDS             8268..>8523
                     /locus_tag="VHM48_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754739.1"
                     /translation="MSILRNLGRRRLRTTLTILGITIGIWALVVFSSMANKINALVAG
                     GTEYFQGKVSVSDSSTQLSGFAAAPMLIVTADQVRGIDGVA"
BASE COUNT         1426 a         2855 c         3031 g         1211 t
ORIGIN      
        1 tcgggcggcg gcgttccggt cgccgggaac atggccgcga cgagcttgcg gatcgcggcc
       61 acgtcgggga cctggatgct gccgcggccg tcgtagtcgc ctttgacgag cggatgggtg
      121 atgacggctc ggtaggtctg cttgcggccg atcttcgtcg cgatgtcggc gagatcgggg
      181 acgagcttgc gcgggatgtt cgtctcgacc gtgtcgctga gggccctgag caacccgatc
      241 gggtcgttga ggaagccgcc cgacttcacc cggtcgcgga tccccgacag gatctcctgc
      301 tgccgtgccg cacgtgtgaa gtcgctctca ccggcggcct tgcggatgcg cgcatagccg
      361 agcgcctgct ggccgttgag gtggtgacgg ccggccgtga tcccgaagcc gtgggtgaac
      421 ccgtactcgt cgtacgtcgg gtcgcagaac ccgtcggcga cgtcgacgtc gatcccgccg
      481 agcgtgtcga cgacgttcac gaacccctgg agattgaccg ctgcgtagtg gtcgatccgc
      541 aggccgagaa gggtgcccaa tgcgcccatc aggacgtcat aacccttgcc gttcgagccc
      601 ggaaaggtcc cctggtgatg gcgggcgaac gaaacgaggc cgttgatctt gccgctgaac
      661 gtgcgcccgt ccggcagcgg cacgtcgacc atgtcgcgcg ggatcgagat catcgagacg
      721 gtctggccca ccggatcgag cgagaccacg atcatcgtgt cggtcaggta ggtattccgg
      781 ccgacacccg catcgacgcc gaccaggagc acgttctggc gaggaacagc cggcgacggt
      841 gaggccgacg acgggacgct gggcgaggcg ggcacctccg acggcagcgt gacctccggg
      901 tccgggacgg ccgacgcgga tgccgacggg tcccacgcgc cggtcgtcgt cgggtcgatc
      961 gggatgatct ggtcggtcgc ctcacgggcg acctcggtcg cgtacgcggc gaacccgtgg
     1021 ggggcgacga ggacgaccgc gaggagcgcg atcggcaacg cgtcgcgtcg gccgagacgg
     1081 gggaagcggc cgtcgaccag gctcgagccg atggcggctg cgcgccagac caggagcagg
     1141 gcctggagac cgaacaggac gacgacggtc gccggatcga gggcgatgcc gagcaggcgg
     1201 ctcgtcggga tcagcagcag gccgccgacg acgacaatga tcgtcacgag cgtcgggatc
     1261 ccgaagagcg cggcgcgacg ccggcgcccg gccgcgagat ggccgagtcc cggtaacagt
     1321 gaatcgagga aggcgtggag gtgacgggac ccccgacgcg gggtgacggc cgcgtcgccc
     1381 tcaggcgggt tgttcacgtc gacggagcct accagaacac accggaccgc tcgcatcccc
     1441 cattcgggcc gggtggtcgc aggacccccc aggcggctcg gtcaggctcg gaacgcctcg
     1501 gcggccgccc aggtgtcgaa gaattcctgg aaccggacga tccggccgtc agcgacggtg
     1561 accacgtgga cgaagtccgt gtcatagacc ttgcccgtcg gacgggcctc gatggtcgtc
     1621 cagccgatga ccgcgacggt gtcgccgtcg acgaggaagc gaaccggctc gaatgcgaga
     1681 atcgtctcgg tctccttgcc gatccggagc cactccagca cgccgtccac gccgaaccgc
     1741 atcccggccg acgggatcag cgggttcggc gcttcgcccc agatgacgtc ctcatggagg
     1801 aggccgagca gcgcgttgat gtcccgcgcg gcaaatgccc gataggcgga ctggacgagg
     1861 gcgaggatct ccgccggcgc cgcttccgcg tcctgaatca ccatggatgt gctcctccgc
     1921 tccgccgacg gctcgcccgt ccgatccagt cgggtccgat cgttcaccgg gcgcgcgtca
     1981 cgggcgaggg cagaacgtcc actcgacggg ggcctttcgg cccgccactc cgcccatggg
     2041 tccgccggcc gtttgagatg ccgctccgcc tggggggctg gcgtcgctga tcgatccgct
     2101 gaccgatccg gcgacgggct tccaccagcg cctcgaggag atcttccacc tcgactgatc
     2161 ggcagaggcg gcgcgtcagg tggtcggcgg cggatccccg gcgggcgaca ccatggcgag
     2221 atgggagggc caccagttgc gtcgaccgag aaggacgacc agcgagggca cgagcagcga
     2281 ccgcaggagg aacgtgtcca tgagcacccc ggccgcgatc ccgtaaccga tctgctggat
     2341 ctggccgccg cccgccgagc cgcccgcgac ggcgaggacg gcgaatgtgc cgccgaggat
     2401 caccccggcc gtggtgatcg tgctgcccgt ccgcccgatc gcccgcgcga cggcatcgcg
     2461 caacggaaga tcgtgggctt cctcgcgaat ccggctcatg atcaggatgt tgtagtccga
     2521 cccgagggcc atcaggaaga cgaacatcag gaacggcagg aggaagttga ttccgtcctg
     2581 cccgccgaga tggacgaaca cgatgccgac gaggccgagg gcggcgaggt aggacaggac
     2641 gatgctcgcg acgagataga ggggcgcgac gaggctgcgc aggacgatcg cgagcaggag
     2701 cgcgatgagc accgcgacga tcgggatgat ccggacgagg tcggcgccgg agatatggct
     2761 gacgtccgag gcgaaggcca cctggccgta cacgccggac gcggtcgcac ccgccgccat
     2821 cgcgacgcgt tcgacatcgg cccgcatcgc cggcaccgag tcgagcgccg cggggctcga
     2881 gaggtcgccg ctggcgaatt cgaccgagaa ctggatggtc cggccgtcgg ggctgatcaa
     2941 ctggcccgtc gcccggtacg cggcgtagag ctggggcgac acatcggccg tcgccggcac
     3001 cggggggagc tcccgggccg gcccaaggtt cgtgtggagc tctgccagct ggtcggtcgt
     3061 gatcgcgatc ccgatcggat tgagtggtcc gaccagaccg ccgaactccg tgagtccggt
     3121 gagcccgtcc tgggcgcggg agaccgagga caggtcactc cagacaggag tcgcgaatgt
     3181 catgaggacg gcgcttcgac tcgcgttcga cgacgggaaa tgctgctcga tcgacgcgga
     3241 tccagcggct gaatcggtac cgctcgggct ggtcgcggcg ccgccgaagc ccgccacgcc
     3301 ggtcgtcagc agggtcgctc cgagcgccgc gaacaggacc actccggcgg cgagcgtcgg
     3361 gccgggtcga cgcgtggcca tgatgccgat gcgatcccac atgccgtgtc gatcgtgggc
     3421 gcgtgccgtg gcaccagtcg gccagaacac cgctcggccg aagatcgcga ggagggccgg
     3481 cagaagcgtc aaccccgcta cgagcatcag gccgatcccg atcgcgaggg cgggtccgag
     3541 gctctggtag aaggcgaact gggcgatggc gaggctcagg agggcggcca tgaccgtgag
     3601 ggccgagaac gtgatcgact cgccgacgcg gctgaccgac tggatcaccg cctcatgggc
     3661 agtgaggccc ctgcggagct cctcccggac ccggaagacc agaaacacgc cgtagtcggt
     3721 cccggcaccg aggatcagca cgatcagcag aagctcggtg atcgaggaca cctggacgcc
     3781 gatcttcgtc gattcggcga tgacggggcc cgacaggatg agcacgaacg ccgccggcac
     3841 gagggtcacg atcggggcga gcacggcccg gaacgcgagc accaggagaa cgacgatgaa
     3901 cagcagcgac agctgctggg tgaggttctg cgacgagccc gacgcggcga cggtgtcgac
     3961 ctgcgtcgcg agctggcccg tcacgtggat ctccaggccg atcggaggca cggacctctt
     4021 gatggtcgcc cggatggcgt cgacgacgcc cttcgcctcg gggccggcgc tgaacgcgac
     4081 gacagcggct tcgacgaggg cctgacgggc ctggccatcg ctcgacacgc cgaggtcgac
     4141 gacgaccttc acccgatcga tgccgcggat ctgcgtctcg agcgcgtcca tggaggcgtt
     4201 atcggcggcg gtcagacccc cgtcacgggc catgacgacc gttgccgcgg ccagggacgt
     4261 gtcctggaac ggcgcggcca tcgcggctgc ctgcatgctc ggcgaatccg caggcaggaa
     4321 gccgctggtc gtgtccttgg ccacgtcgcc gagtgacggc aacaactgga ccgcgaggac
     4381 ggtcacgacg acccagacga cgacgatcag gaaccggaag cggaccgcga agcgcccgag
     4441 gcccgtgaag aaacggctca tgagagtcct ttcgtgacca ggcccgccgg cccgcacgcg
     4501 tcggcgggtc gatcgggccg ttgggtggat gttttcgctc agaacggggc gatgacgaag
     4561 cgcccgaagg cgacgttagg cggccagcac cgcaaggaca agattggcgg cgatgttccg
     4621 gccttcgccg ggccgccggg tgtggaacgc catggcgagg agcatcagga tgacgcagca
     4681 ggtcgccaca atcggggcag ctcgagcgat ccgatcgctc gcatcggggt tcgcccgatc
     4741 gcgcccagcc aggtcgtccc gggcctcgtc accgactgat cgaacgcgat cgagtgaccg
     4801 tagccgaacg ccaggagacc taaggccacc acggcctgat cgatccagag aagaacgttc
     4861 gggtccacgg cttctccctc agatcccgct gggctcgagg attggcgcga tcgccatcag
     4921 agcgggagca gcgcggcggc cacacccacg accactccga cgacgaggct ggccactccg
     4981 atctgtgtgg ctcgggcacg atcggcggcc cggtcgacgg ggtccgcgac gccctcggtc
     5041 tggccaggga cgccgaagtt gaagacgcct cgtgaaccga acccggcaca gaatcggagc
     5101 caagcctgaa gatagccgga cgccgcgccg gcagccggca ggacgaggac gagccgggtc
     5161 agcggtggcg caccgaccgc gacgagggtg gccaggagga tgacggtcac ggccaaaccg
     5221 acacggccgg cagttcgtcg cctcgcgatc tcggccgggc cgatgttgca gaccccgggc
     5281 tcataggacg aacgagccgg accggcaccg ggcgcctccg gcaggccaac tgctgcgagc
     5341 gctgactgca tcgttggccg ccctcggatt cgtccgcgtc ccgcgtgagc gcggacctcg
     5401 gcgtggggtt gcttgcgttg aaacaatggc agtctatcga caatcattgt gtagcgtcaa
     5461 tgattgcccg ctgctaacct gagcagccat ggacgaggcc gacgatcgcg aggagctcgc
     5521 gcgccgcggg tggcgcctcc tgatgggcgc cggccagcgc catctggagg tcgtcacggc
     5581 cggaatcgag gagatgggcc tgagcaaggt gatggcccag ttcctcggcg ccgtctgtca
     5641 gtcgccaccg ggcccgacga accagctcgc gacccgcttc ggggtcgatc cggggtgggt
     5701 caccgacatc gtcgaccgac tggaggctcg cgggcagctc gttcgtcgcc agtcgccgaa
     5761 cgaccggcgg gtcaagatcc tcgacgtgac cgaagccggc cgcgagacat tccgcgccat
     5821 ggaggcgatc ttcgcgacac cgccacccga gctgctcgac gcgtctcggg aggacctcct
     5881 ggcgctcgtc cggatcgccg agcgcctcgc cggtccgatc gaaccggtcg acgccgtcga
     5941 cccgtgagtt gacctcgctg gcggcgtcga gcggctcatc gagcgcccgg cgacagcgcc
     6001 cggcgacagc gcccgccggt ggtcaggccc cgttccgcgt ccgttcgagg ccgtcgagga
     6061 gcaggtcgag gccgtactcg aactcgtctc cgaagtcgta gccgggcttc atgacgtgtt
     6121 cggtgatgaa cgccacgagg ttggggtact cgttcattgg gaacggctcg agcatggtct
     6181 gggccacctc ggcaacgttg tccccgcttt cgaacgggag gttcatcttc gtcagggcga
     6241 agccgtagat gtagccgtcg agaagcgagt acgcgtgcgc gaccagctcc atgtcgaagc
     6301 ccgccgcgcg gagacacccg atcaccgcgt cgtggtggcg caggctcgcc gggccagggt
     6361 tgacccgcga ctccatcagg ccgatggccc agcggtggcg cgacaggacg tcgcggagcg
     6421 agatcgcgcg ccgccgcatg gccgtcctcc agtcggcgcc gcttgacggc agatcgacct
     6481 cgctcacgac gacgtcgacc atcccgtcga cgagatcgtc cttgttcgcg acgtggcggt
     6541 acagcgccat cggggcgacc ccgagctcct cggcgagcgt gcgcatgctg agcgcctcga
     6601 gcccgcccgc gtcggcgtgg gcgatcgcag cgcgaaccac tcgctccctg ctcaggcggc
     6661 tgcggggctc cgctgtcgaa ttcgtgcgtg tcgccatggg caccactccg tcctcttgtt
     6721 gcgtttcggg tccatcgcga aattcggggt tgacagcgta catcgtacac attagcgtac
     6781 gccgtacacc agcagcgtac atcgtacgca ggcaaggtgc aacaggaagc ggagacccag
     6841 ccatgcaggc aatcgtccag gacagctacg gctcgcacga ggcgctcaag gtcggcgacg
     6901 tggacaagcc cgagatcggc gccgacgagg tcctggtccg cgtccaggcg gcgtccatcc
     6961 atgtcggtga ctggatcctc atgaccggct cgccgttcgt catgcgcctg gccaccgggc
     7021 tgcgcaagcc gaagaaccgc gtgccgggaa cggacgtcgc ggggacggtc gaagcggtcg
     7081 gcagccgcgt ccagcgcctc gcagtgggcg acgacgtctt cggctggtgc gcgggcgcct
     7141 tcgccgagtt cgcgcgtgcg agcgaggacc agttcgtgaa gaagccggcc aacctcacgt
     7201 tcgagcaggc ggccgcggtc ggcgtctcag ccacgaccgc cctccaactg cttcgggacg
     7261 acggccggat ccggtccggg cagaaggtcc tgatcaacgg ggcgtccggc ggcgtgggca
     7321 cgttcgcggt gcagatcgcc aaggcgtccg gtgccgaggt gaccggtgtc tgcagcacga
     7381 agaacgtgga catggtccgc tccatcgggg ccgaccacgt catcgactac acccgcgagg
     7441 acttcacgaa gggcaccgag cggtacgacc tcatcctcga caacgtcggc aaccactcga
     7501 tggcccggac ccggcgcgtg ctgacccccc tcgggaccct gatctcgaac ggcggcgggc
     7561 actccgacgg caagctgggt cgcaccgtgc ggaccatgct cgtctcgatg ttcgtgcgcc
     7621 agcaggcgag cccgacggtc aagacccaga accgcgagga cctcgtcgcg ctgaaagggc
     7681 tcgtcgaagg cgggaaggtg acgcccgtca tcgaccggac gtacccattg accgagaccc
     7741 ccgaggcggt cggtcacgtc ggagcgggac acgcccgcgg gacggtcgtc atcaccgtct
     7801 gacgcaccgc ccgcccgcgg ccgcgtcgtt ctcgaatcga accactgaaa ggaaccgacc
     7861 atgtccaaag caaccgtgac gagactcttc atcggaagcc tgatcgcctt cgccggcggc
     7921 gccatcctgg ccgtcgcggc cgtgtggctg gcaatggcga acgacgtgtt cgtgatggcc
     7981 ggtcccgaca tcgtcgggct ccggggcagc gccttcgcct ggtccctcct cggcctcggc
     8041 atcgtcggcg gcgtggcgat ggggggcgcg ctgatcggtg gcctcgtgtc gtggatcggc
     8101 gccctgctga acacctggca gctcgagagc cggacgtggt tcgcggtgct gctgctgctc
     8161 ggcatcttca atgtcgggtt cctcgcgatg atcgcctacc tgatcgccgg gccggacagc
     8221 acggcggcag tgaccccgcg cggggcgcac gcgcccgtcg cagcctgatg agcattctgc
     8281 ggaatctcgg ccgccgcagg ctgcggacga ccctcacgat cctcggcatc accatcggca
     8341 tctgggcgct cgttgtcttc agctcgatgg ccaacaagat caacgccctg gtcgcgggcg
     8401 gcaccgagta cttccagggc aaggtctccg tcagcgacag ctccacccag ttgagcggct
     8461 tcgcggccgc gcccatgctc atcgtgacgg cagaccaggt ccggggaatc gacggcgtgg
     8521 ccg
//
LOCUS       DATAIW010000148         9313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_67350, whole genome shotgun sequence.
ACCESSION   DATAIW010000148 DATAIW010000000
VERSION     DATAIW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67350"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VHM48_04720"
     CDS             <1..263
                     /locus_tag="VHM48_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2754740.1"
                     /translation="LGHDRAIDRPIVAQSARNRFAGIVTRIEKDRVAAVVEVQAGPHR
                     LVSLMTAEAVTEMGLKVGDEAICVVKSTSVIVEVPSPRETRG"
     gene            complement(503..859)
                     /locus_tag="VHM48_04725"
     CDS             complement(503..859)
                     /locus_tag="VHM48_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754741.1"
                     /translation="MGSPKLRSALRALGITRRESTSAFALIPTARGHVMAEAVHAPSA
                     AGDALRIAVRDSAFVERGTTWGERAVAGRPIDWAVGRRFEVAVFTDDQGVAFHLAGEH
                     GSVAFAAEQLLASTRV"
     gene            complement(979..1968)
                     /locus_tag="VHM48_04730"
     CDS             complement(979..1968)
                     /locus_tag="VHM48_04730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX2754742.1"
                     /translation="MTDRPKVFLARLIPDDGIAPIVAACDARVWEDESPPPRAALLDA
                     IRGCDGVVTLLTDKVDDEFLDSAGPQLKVVSNFAVGFDNVDVPACTARGVAVGNTPGV
                     LTETTADLAWALLMAAARRLPEGDRYVRAGKWKTWGPLLLLGPDVHGATIGIVGFGRI
                     GQAVARRAQGFGMTILYHDVHRADPAVEAEFAATFLPLEELLPRADYVSLHVNLTHDT
                     HGLIDAEKLGWMKPTAVLVNTSRGPVVNGQALADALRSGSIFAAALDVTDPEPIPADD
                     PLLSLDNCLVVPHIASASRATRSKMAEMTAANLLAGVRGERIPTPVNPEVYDR"
     gene            complement(1965..3170)
                     /locus_tag="VHM48_04735"
     CDS             complement(1965..3170)
                     /locus_tag="VHM48_04735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2754743.1"
                     /translation="MRLAQRMSVIGTETAFEVSARARALEAEGRDIIHLQIGEPDFDT
                     PANVREAAKKALDEGATHYAPYPGIPALREAIADDAQARKGFPVTPDRVFVTVGGKGV
                     MLYAILGLIDPGDEVLVPDPGYPIYESLTRFVGGTPIPVPIRMEHDFRIDLDELASLV
                     TPKTRMLVINSPANPTGGVLTRGDLESIAAIAIRHDLVVLDDEIYGRILYDGEEHVSI
                     ASLPGMAERTIVLDGFSKTFAMTGWRLGYAIVPPSLVAMYGQLVINTISCAPTFAQVG
                     AVEAIRGPQEPMAAMVEEFRARRDLVVDGLNDIPGIRCLRPKGAFYAFPEISGTGLGG
                     KELADRLLVEAGVCVLAGTAFGGIATNHIRVSYANSQENLTKALGRIGEFVAGLPTGA
                     SPAAAGAAR"
     gene            3265..3780
                     /locus_tag="VHM48_04740"
     CDS             3265..3780
                     /locus_tag="VHM48_04740"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX2754744.1"
                     /translation="MTRFFDLDEANAALPEVRAILESLRDERVELIRLRDQVLLHRSP
                     SDAPASAPTEANVGGASRPAEPVETQDGVAASGPGAGPTDDPAAHDPEVRILRLRMQG
                     VIDRMQAGVARIDELGVTLREIETGLIDFPALATGRQIWLCWRLGEGDIGFWHELGDG
                     FASRQALVDLT"
     gene            3777..4211
                     /locus_tag="VHM48_04745"
     CDS             3777..4211
                     /locus_tag="VHM48_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX2754745.1"
                     /translation="MSGATVVQNGRAKAYRPLSADARRRAYDAALEAYDRGDFFETHE
                     LLEPAWMGTDDLAERALHQGLIKLAAAFVHGVRGNPLGIARNLVGAREHLATAIGSPP
                     AAACGLDLAALVSAIDDRLARLAADPTDASIEAPALPRSHPR"
     gene            4208..4609
                     /locus_tag="VHM48_04750"
     CDS             4208..4609
                     /locus_tag="VHM48_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2754746.1"
                     /translation="MMPAGVPSIAPRDAAAATDPSTATDPATRPLIVDVREADEFSRE
                     RVAGAVLLPISQFVARHRELPKDRPLLMLCHSGSRSTSATMYLLQSGWPDVRNVTGGI
                     IGWRYAGLPIVTGAPAPGEGDLEPAGPLAPR"
     gene            complement(5013..6476)
                     /locus_tag="VHM48_04755"
     CDS             complement(5013..6476)
                     /locus_tag="VHM48_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754747.1"
                     /translation="MAQQALHPGTRQTRGRAFFGLLDANGWGWASLKAAFWFILLIFM
                     LGYVPDRAYYFTVNKTIDLGILAWSPLNFCPEGNKTLPCPAPAGAVVPWSTSPAEISL
                     PAPRTDGAIVQSGTTILYVGGSDGTTVSDKTFIARSSGTGNFDKWADGPKLPEPRSDL
                     SVIYSGGKIYAIGGVGADGKPTTSVFVLTPDPKTGALGDWQTAEAAKLNLTLPEARAG
                     AAVVAASDGLLLVGGTNGTAIVDTVLKTTFDTKGVPSKWEAQPGKLFTPVTDASAAVL
                     GSYLWVFGGAAPGVAAIPSVQRGEFGTGADATKVIRFGVKGGATDLPQPRTNMAGFGA
                     NGALYAIGGSDGSKPQGALYWATPTNAGDVTEWKHLDVSDLPSTGVAGGAPIILGPNV
                     IVVGGTTSGGVIAASNRANLAPEAPFFQLGLVGATVPALKIDGEIGQQLGYLNANGIG
                     MANFAIFLFVGWAFAHRDRIKEWRERRRRESEIRKRA"
     gene            complement(6537..7703)
                     /locus_tag="VHM48_04760"
     CDS             complement(6537..7703)
                     /locus_tag="VHM48_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2754748.1"
                     /translation="MTDQTPAPVPGRAPEPGRGAEERLPATVPQGSLSADRFSAPPSI
                     KANAGLTPQRSAGIVRQSASARWVGLLTVIFVSLFIVGYWFYELGAPAGISKPRLATE
                     IEAQQVTDVERGYNLFEANCARCHGPGGLGPNEPDAAAKSAAGQGYIGPQLNAQEKLF
                     AHLNAQYLRNVLTVGGRYVCGNANSAMPVWSDKGSPAGPLNYRQIDELIAFLRATSDK
                     TYDIRDGATNEPVIDPATGKVKTFTGWRDLNYKPAAGATPYPDCYLKALTGGAGSSGA
                     PASGGPAASVDANAPTVTLTAENGAATAGFKETTLTAKADTAFSFVFDNQDPTVPHNV
                     VITDPGGSPVAMGDTSPFQGPEKRTYSVPALHAGTYTYVCQVHPSTMKGTLEVK"
     gene            complement(7703..8377)
                     /locus_tag="VHM48_04765"
     CDS             complement(7703..8377)
                     /locus_tag="VHM48_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2754749.1"
                     /translation="MSNYHVEPIDRPKALAPEAIKSLRRAQVKGVSRRQLLRYSLGGA
                     VGLWFFEVLAGTLGFVWPNLSGGFGSKVRIGTLDDLKLANSSLPISDGFPAYVADARA
                     YIMLVDTGQQQFVAGDDPSGDGRALNVRGLYQRCPHLGCKPNPCLKNFWFECPCHGSR
                     YDRLGIKAAGTQFGPAARGMDRFSASVDGGGVLTLDTGKITLGPLPVAVGQPGIIPPR
                     TPTGCI"
     gene            complement(8429..9001)
                     /locus_tag="VHM48_04770"
     CDS             complement(8429..9001)
                     /locus_tag="VHM48_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754750.1"
                     /translation="MDPARRTEIDKQRVPMSARPYRLLALVKQDAVVRVQKEPDDTVM
                     TWPHLLSIEFLAAGMMSILLLLMGLFINAPLEELANNNVTPAVAKAPWYFLGLQELLA
                     YFHPTVAGVLTPLLVLVGAALIPFVDRGNLTVTRPSERKTAVILFTMFCALGLILTFV
                     GIFFRGPGYSFVIPYLPFFENTSPGLHFSL"
BASE COUNT         1386 a         3179 c         3380 g         1368 t
ORIGIN      
        1 gactgggcca cgatcgggcg atcgatcgcc cgatcgtggc ccagtcggct cgcaaccggt
       61 tcgcaggaat cgtgacccgg atagagaagg accgggtcgc cgcggtcgtc gaggtccagg
      121 ccgggccgca tcgcctcgtc agcctgatga cggccgaggc cgtcaccgag atgggcctga
      181 aggtcggcga cgaggcgatc tgcgtcgtga agtcgacgag cgtgatcgtc gaggtcccgt
      241 caccgcgcga gacccgcggc taggaggccc gatcgcatcg gacgcgcgac gccgggtccc
      301 gaatgctcgc ctgacgagca ttacgacgtc cgagcgaccc gggcaggagc ccacgcggcc
      361 cgaatcgacc ctcgatcagc actccagggc taacagcacg cctcaggctg ccgccgacgg
      421 cgcgcccagg cgccgtaacg ctcaccacgc acgcatttgg ggctcagcgg gcagtccgca
      481 tgccgcggcg accggacccg gttcagaccc tcgtcgaagc gagcagttgc tcggctgcga
      541 acgccacgga tccatgctcc ccggcgagat ggaacgcgac gccttgatca tcggtgaaga
      601 cggcgacttc gaaccgccgt ccgacagccc agtcgatcgg gcggccggcg acggcgcgct
      661 caccccaggt cgtgccacgt tcgacgaagg cgctgtcgcg aactgcgatc cgcaacgcgt
      721 cgcctgcggc gctgggcgca tggacggcct ccgccatcac gtggccgcgg gccgtgggga
      781 tcagcgcaaa ggcgctggtg gactcccggc gcgtgatccc gagcgcacgg agggcagatc
      841 gcagcttcgg cgaacccacg ccgcgcctcg acatcaagga tgtggcgccc gaggctcgag
      901 ccaaccgtgc ttcgaggatg acgtcgtcgt gcggcggacc gaagccgggt ggtggattgt
      961 cgatccgaaa cgagaacgtc aacggtcgta gacctccggg ttgaccgggg tcgggatgcg
     1021 ttcgccgcgg acgccggcca ggaggttggc cgcggtcatc tcggccatct tcgaccgagt
     1081 cgcccgcgag gcggacgcga tgtgcggcac gacgaggcag ttgtcgaggg acagcagcgg
     1141 atcgtcggcc gggatcggct cggggtcggt cacgtcgaga gcggcggcga agatcgagcc
     1201 ggatcggagc gcgtcggcca gcgcctggcc gttgacgacc gggccgcgcg acgtgttcac
     1261 gagcacggcg gtcggcttca tccagccgag cttctcggcg tcgatcaggc cgtgggtgtc
     1321 gtgggtcagg ttgacgtgga gcgagacgta gtcggcccgc ggcagcagct cctcgagcgg
     1381 caggaacgtt gccgcgaact cggcctcgac cgccggatcc gcgcgatgga cgtcgtggta
     1441 caggatcgtc atcccgaatc cctgcgcccg gcgggccacg gcctggccga tccgtccgaa
     1501 cccgacgatc ccgatcgtcg cgccatggac gtcaggcccg agcagcagga gcgggcccca
     1561 ggtcttccac ttcccggccc ggacgtagcg gtcgccctcg ggcagccgcc gcgcggcggc
     1621 catcaggagc gcccaggcga gatcggcggt cgtctcggtc aggactcccg gtgtgttgcc
     1681 gacggcgaca ccgcgcgcgg tacaggccgg gacgtcgaca ttgtcgaagc cgacagcgaa
     1741 gttcgacacg accttgagct gcgggccggc ggagtcgagg aactcgtcgt cgaccttgtc
     1801 ggtgaggagg gtcacgacac catcgcagcc acggatcgcg tcgagcagcg cggccctggg
     1861 aggcggcgac tcgtcctccc agacccgtgc atcgcaggcg gcgacgatcg gggcgatccc
     1921 gtcgtccggg atcaaccggg cgaggaagac cttcggccgg tcggtcatcg ggccgcgccg
     1981 gctgccgccg gcgacgcgcc cgtcggcagc cccgccacga actcgccgat ccgcccgagc
     2041 gccttcgtca ggttctcctg cgagttcgcg tacgagacgc ggatgtggtt cgtcgcaatg
     2101 ccgccgaacg ccgtcccggc gaggacgcag acccccgcct ccaccagcag ccggtctgcg
     2161 agctccttcc cgccgagccc ggtcccggag atctccggga acgcgtagaa cgcgcccttc
     2221 ggtcggaggc accggatccc ggggatgtcg ttgaggccgt cgacgaccag gtcgcgccgc
     2281 gcccggaact cctcgaccat cgcggccatc ggctcctgcg ggccgcggat cgcctcgacc
     2341 gccccgacct gggcgaacgt cggggcgcac gaaatcgtgt tgatcacgag ctggccgtac
     2401 atcgccacga gcgacggcgg cacgatcgcg tatccgagcc gccagccggt catggcgaag
     2461 gtcttggaga agccgtcgag gacgatcgtc cgctcggcca tgcccggcag cgaggcgatc
     2521 gagacgtgct cctcaccgtc gtacaggatc cggccgtaga tctcgtcgtc gagaacgacg
     2581 agatcgtgac ggatcgcgat cgcggcgatc gactcgaggt cgcctctggt caggacgccg
     2641 ccggtcggat tggccggcga gttgatcacg agcatccggg tcttcggggt gacgagggag
     2701 gccagctcgt cgagatcgat ccggaagtcg tgctccatcc ggatcgggac cgggatcggc
     2761 gtgcccccga cgaaccgggt cagggactcg tagatcgggt agccgggatc ggggacgagg
     2821 acctcgtcgc ccgggtcgat caacccgagg atcgcgtaga gcatcacgcc cttgccgccg
     2881 acggtcacga atacccggtc gggcgtgacc gggaagccct tccgggcctg ggcgtcgtcg
     2941 gcgatcgcct cgcgcagcgc gggaatgccc ggatacggcg cgtaatgggt ggcgccctcg
     3001 tcgagggcct tcttggcggc ctcgcggacg ttcgccggcg tatcgaaatc gggctcgccg
     3061 atctggaggt ggatgatgtc gcggccctcg gcctcgaggg cgcgggcccg ggcggagacc
     3121 tcgaacgcgg tctcggtccc gatgacgctc atccgctggg cgaggcgcat gtcgagctcc
     3181 ttgtggggtg gtccgcgcag tctaacgcgg ccccacatcg ggcccatcgc cgggccgacc
     3241 gcagcgacgg gctagaatca cggcgtgacc cgattcttcg atctcgacga agcgaatgcc
     3301 gccctgcccg aagtccgggc gattctcgaa tccctgcgcg acgagcgggt cgagctgatc
     3361 cgactgcgcg accaggtcct gctccatcgc tcgccgagcg atgcgcctgc cagtgccccg
     3421 accgaggcga atgtgggtgg cgcctcgcgt cccgcggaac ctgtggagac tcaggacggc
     3481 gtggcggcat ccggacccgg agccggcccg accgacgacc cggcggctca cgacccggag
     3541 gtccggatcc tgcgcctccg gatgcagggc gtgatcgacc ggatgcaggc cggcgttgcc
     3601 cggatcgacg agctcggcgt cacgctccgc gagatcgaga ccggactgat cgacttcccc
     3661 gcgctcgcga ccggccgcca gatctggctg tgctggcggc tcggtgaggg cgacatcggg
     3721 ttctggcacg agctcggcga cgggttcgcc agccgccagg cgctggtcga cctgacgtga
     3781 gcggcgccac ggtcgtccag aacggccggg ccaaggcgta ccggcccctg tcggccgacg
     3841 cccgacggcg cgcttacgac gcggccctcg aggcctacga ccgcggcgac ttcttcgaga
     3901 cgcacgagct cctggagccc gcctggatgg gcaccgacga cctcgccgag cgggccctcc
     3961 accagggcct gatcaagctc gccgcggcgt tcgtccacgg cgtccgcggc aacccgctcg
     4021 ggatcgcccg gaacctggtc ggcgcccgcg agcacctggc caccgccatc ggctcgccgc
     4081 cggcggcggc atgcgggctc gacctggcgg cgctcgtcag cgcgatcgac gatcggctgg
     4141 cccgcctcgc cgccgatccg accgacgcct cgatcgaggc gccggccctg cccaggagcc
     4201 atccccgatg atgccggccg gcgtcccgtc gatcgctccg cgggacgcgg ccgcggccac
     4261 ggacccgtcg accgccaccg atccggcgac gcggccgttg atcgtcgacg tccgggaggc
     4321 ggacgagttc tcgcgggagc gggtcgcggg cgccgtcctg ctgccgatct cgcaattcgt
     4381 cgcccgccac cgggagctgc cgaaggatcg gccgttgctg atgttgtgcc actccggcag
     4441 ccgatcgacg tcggcgacga tgtacctgct ccagtccggc tggccggacg tccggaacgt
     4501 caccggtggg atcatcggct ggcgctacgc ggggctgccg atcgtcaccg gcgccccggc
     4561 cccgggcgag ggcgacctcg agcccgccgg tcccctggca ccgcgctgac cgaaacgcca
     4621 cccgcctggc gggtggatcc agcggtgcat cgacgcgaac gcccctggca ctcgggcctc
     4681 gaagcggagc agatcaccgg gagatcaacg ggactgaacc gccctgggtt tcgtggagtc
     4741 cgggctgatc gggtttcagg cggcgtcgcc cgactcggat cgccaacccg cgcccggatc
     4801 gcggcctcct gatcggtctc attgatcgaa tcggcgcctg gcccgcacga ccaggtggct
     4861 gcatcctcag gtgcgcgcat gatcggccac gcgggcacgc tcgctcgcac cggatccgat
     4921 caatgacggc gatcacgatc gccgcgcggg ccgcgtccgg ggcgggcgct catggcgtgg
     4981 tccgcgccgc gcgggccgcg gcccctcccc gctcaggcgc gcttgcggat ctcgctctcg
     5041 cggcgtcggc gctcgcgcca ctccttgatc cggtcgcgat gggcgaacgc ccagccgacg
     5101 aacaggaaga tcgcgaagtt cgccatgccg atgccgttcg cgttgaggta gccgagctgc
     5161 tggccgatct caccgtcgat cttcaacgcc gggacggtcg ctccgaccag gccgagctgg
     5221 aagaacgggg cctccggcgc aagattggcg cggttgctcg ccgcgatcac gccgccggac
     5281 gtcgtcccgc cgacgacgat gacgttcggt ccgaggatga tcggtgcgcc gccggcgacg
     5341 ccggtgctcg gcaggtcgct gacgtcgagg tgcttccatt cggtgacatc gccggcattc
     5401 gtcggggtcg cccagtacaa ggcgccctgc ggcttcgacc cgtccgatcc gccgatggca
     5461 tacagcgcgc cgttcgcgcc gaagcccgcc atgttggtcc gcggctgggg gaggtcggtc
     5521 gcgccgccct tcaccccgaa ccggatgacc ttcgtggcat cggcaccggt cccgaattcg
     5581 ccgcgctgga cgctcgggat cgccgcaacg ccgggggccg ctcccccgaa gacccagaga
     5641 tagctgccca ggaccgcggc cgaggcgtcc gtcaccgggg tgaacagctt gcccggctgg
     5701 gcctcccatt tgctcggcac gcccttcgta tcgaaggtcg tcttgagcac ggtgtcgacg
     5761 atcgcggtgc cattcgttcc gccgacgagg aggagtccgt ccgaggccgc gacgaccgcg
     5821 gcaccggccc gagcctcggg cagcgtcagg ttcagcttgg cggcttcggc cgtctgccag
     5881 tcaccgaggg ctcccgtctt ggggtccggc gtgaggacga acaccgacgt cgtcggcttg
     5941 ccgtccgccc cgactccgcc gatcgcgtag atcttgccgc cggagtagat gaccgacagg
     6001 tccgagcgtg gctcgggaag cttgggcccg tcggcccatt tgtcgaagtt cccggtcccc
     6061 gagctcctgg caatgaaggt cttgtccgag accgtcgtcc cgtccgagcc gccgacgtac
     6121 aggatcgtcg tgcccgactg gacgatcgcg ccgtcggtgc ggggcgccgg caacgagatc
     6181 tcggcgggtg aggtcgacca cggcacgacc gcgccggcgg gcgccgggca gggcagggtc
     6241 ttgttgccct cggggcagaa attgagcggg ctccaggcga ggatgccgag gtcgatcgtc
     6301 ttgttgaccg tgaagtagta cgcccggtcc gggacgtagc cgagcatgaa gatcaggagg
     6361 atgaaccaga aggctgcctt cagcgacgcc cagccccagc cgttcgcgtc gagcaggccg
     6421 aagaacgccc gtccgcgcgt ctgtctggtt cccgggtgca gggcctgctg cgccatcgtc
     6481 ctccatccga ccgggcggtt gcgccgcccg gctcttccag ccgcctggtg tgctgcctac
     6541 ttgacctcga gggtcccctt catcgtcgag gggtggacct ggcagacgta ggtgtaggtg
     6601 cccgcatgga gcgccggcac cgagtacgtc cgcttctcgg ggccctggaa cgggctcgtg
     6661 tcacccatgg cgaccggcga gccccccgga tcggtgatga ccacgttgtg ggggaccgtc
     6721 gggtcctggt tgtcgaacac gaagctgaat gccgtatcgg ccttggcggt gagggtcgtc
     6781 tccttgaagc cggcggtcgc ggcgccgttc tcggcggtga gcgtgacggt cggcgcgttg
     6841 gcatcgacag aagcggccgg gccccccgac gccggcgctc cggacgagcc cgcgccaccg
     6901 gtcagcgcct tgaggtagca gtccgggtac ggcgtcgcgc cggcggccgg cttgtagttc
     6961 aggtcgcgcc agccggtgaa cgtcttgacc ttgccggtcg ccgggtcgat caccggctcg
     7021 ttggtcgcgc cgtcgcggat gtcgtaggtc ttgtcgctcg tcgcccgcag gaacgcgatc
     7081 agctcgtcga tctggcggta gttcagcgga ccggccgggc tgcccttgtc cgaccagacc
     7141 ggcatcgccg agttggcgtt gccacagacg tagcgcccgc cgacggtcag gacgttgcgc
     7201 aggtactgcg cattgaggtg ggcgaacagc ttctcctggg cattgagctg cgggccgatg
     7261 tagccctggc cggcggccga cttggcggcg gcgtcgggct cgttggggcc gaggccgccg
     7321 ggcccgtggc agcgcgcgca gttcgcctcg aacaggttgt agccgcgctc gacatcggtg
     7381 acctgctggg cctcgatctc cgtcgccagg cgtggcttcg agatcccggc cggtgcgccg
     7441 agctcgtaga accagtagcc gacgatgaac aggctcacga agatgaccgt gaggaggccg
     7501 acccagcggg ccgacgccga ctggcggacg atcccggccg agcgttgcgg ggtcaggccg
     7561 gcgttggcct tgatggaagg aggcgcgctg aaccggtcgg ccgacagcga cccctggggg
     7621 accgtggccg gaagccgctc ctcggcgcct cggcccggtt cgggggccct gcccgggacc
     7681 ggcgccggtg tctggtcggt catcagatgc agcccgtcgg ggtgcgtggc gggatgatgc
     7741 cgggctggcc caccgcgacc ggcagcgggc cgagcgtgat cttgccggtg tcgagggtca
     7801 ggacgccgcc gccgtcgacg ctggcggaga accggtccat cccgcgggcg gcgggtccga
     7861 actgggtgcc ggcggccttg atcccgagcc ggtcgtagcg cgagccgtgg cacgggcact
     7921 cgaaccagaa gttcttcagg caggggttcg gcttgcagcc gaggtggggg cagcgctggt
     7981 agaggccccg gacgttgaga gcccggccgt cgccgctggg gtcatcgcca gccacgaact
     8041 gctgctggcc ggtgtccacg agcatgatgt aggcccgcgc atcggcgacg taggccggga
     8101 acccgtcgga aatcggcagg ctgctgttgg cgagcttgag gtcgtcgagg gtcccgatcc
     8161 tgaccttgct gccgaagccg cccgagaggt tcggccagac gaagccgagc gtcccggcca
     8221 ggacctcgaa gaaccacagg ccgaccgcgc cgccgagcga atagcggagc agctggcggc
     8281 gcgagacgcc cttgacctgg gcgcgccgaa gggacttgat cgcctccggg gcgagcgcct
     8341 tcgggcggtc gatcggctcg acgtggtagt tgctcatgcg cggtcgctca tcgggctcat
     8401 cgggtcatcg gtctctcggt ggaggggatc acagggagaa gtgcaggcct ggcgaggtgt
     8461 tctcgaagaa cggcaggtac gggatgacga agctgtagcc ggggccgcgg aagaagatcc
     8521 cgacgaacgt caggatcaat ccgagggcgc agaacatcgt gaacaggatg accgccgtct
     8581 tccgttccga tgggcgggtg acggtcaggt tgccgcgatc gacgaacggg atcagggccg
     8641 ctcccacgag gacgagcagc ggggtcagga cgcccgcgac ggtcgggtgg aagtaggcga
     8701 gcagctcctg gagcccgagg aagtaccacg gcgccttggc cacggccggc gtgacgttgt
     8761 tgttcgccag ctcctcgagc ggggcgttga tgaagaggcc catgaggagg aggaggatgc
     8821 tcatcatgcc ggccgcgagg aactcgatcg agagcaggtg cggccaggtc atgaccgtgt
     8881 cgtcgggttc cttctggacc cggacgaccg catcctgctt gacgagcgcg agcagccgat
     8941 acgggcgggc cgacattggc acccgctgct tgtcgatctc cgtccgcctg gcggggtcca
     9001 ccggcagaac cactcgacgg tcggtgggct gcttcgtatc agtcatggcg caccgaggtc
     9061 cacggccgag ctccgtcgag gccagtggcc cgaggcaaga cgacccgctt gtcaccggag
     9121 cgagcggcct cggatggccg taagcgagga gcgcagggcg caacgcggcg atcggctgtc
     9181 atagcgggcc gctgatgccg ccatccttgc ggatgcgcca gaagtgcacc gccaggaaga
     9241 tggcggcaac gagcgggaag aacatgatgt ggaggacata gaaccgcagg agcgtgttct
     9301 ggccgacctc cag
//
LOCUS       DATAIW010000149        13117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_96993, whole genome shotgun sequence.
ACCESSION   DATAIW010000149 DATAIW010000000
VERSION     DATAIW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96993"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VHM48_04775"
     CDS             <1..896
                     /locus_tag="VHM48_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2754751.1"
                     /translation="TSGATPSPSSSAAPSVAASTAPSVAPSVAPSATPDACAKDNLKL
                     TTGGKLTIGTDNPAFPPYYDAVDSPTKPWQLGDPTDGKGFESAFAYALAAKLGFDKAD
                     VTWIVVPFDNSFAPGTKKFDLDINQVSFTADRAKAVDMTDGYYSLNQSVVALKANGLA
                     SVTSIAGMKGFKFGAQIGTTSLDTINNVIAPTAAAKVYTTNDDAIAGLKAKQIDGLVV
                     DLPTAFYVTAVQVANSVIVGQFPAPTGADAEHFSVVLAKGSPLTACVNTAIAAMKSDG
                     SLDAITKEWLSDKASAPVLQP"
     gene            921..1805
                     /locus_tag="VHM48_04780"
     CDS             921..1805
                     /locus_tag="VHM48_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2754752.1"
                     /translation="MSITGGPLDTGDLIGLAGRRGPRRSRRRMPPGEAVRSSLIALLS
                     TVVVIGGLAVLVTSSPNWPDVRDAFFSDKWFGFSWPLLIDAFWTNVALFLIAELLILP
                     LALVLAIARGLPGPAFFPVRLLATFYVDFFRAVPGLLIIAILGFGIPALDLPGVPNDE
                     FFWAVIALTLVYSAYVSEVYRAGIESVHWSQEAAARSLGLNRLQALRHVVVPQAVRRV
                     IPPLLNDFIGLQKDTVLVSSLGVVEIFREASIIKAAHFNFTPYLAVALIFVILTIPLA
                     RLTDWLVSRDAARQASTR"
     gene            1802..2563
                     /locus_tag="VHM48_04785"
     CDS             1802..2563
                     /locus_tag="VHM48_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2754753.1"
                     /translation="MSMARPREALRIEGLHKSFGDLEVLRGIDLAMAEHEVVCLIGSS
                     GSGKSTLLRCINLLEPIDAGRIVVEGDEITAKGVDVNRIRRRIGIVFQAFNLFPHMSV
                     LANVTLAPRKVLGLSAAEADARATELLDRIGLADKRREYPDRLSGGQQQRVAIVRALA
                     MQPDLLLLDEITSALDPELVAEVLILIRELAGNGMTMLIATHEMSFAREIANRVCFLD
                     AGRILETAPPAEMFSAPREERTKQFLERVIAAGRL"
     gene            complement(2576..2962)
                     /locus_tag="VHM48_04790"
     CDS             complement(2576..2962)
                     /locus_tag="VHM48_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX2754754.1"
                     /translation="MPSPSGSTAPTARPPIVRPTPTPAPTFLVYVVKSGDSLNTIAHR
                     FGTTARSIAFWNRSTYPSLDPESPGYRPNLLKLGWTLFLVPNDVVDEQDLPDPAATGP
                     TDATAAPTDAAAIEDPAAPEDSAIPE"
     gene            complement(3186..6221)
                     /locus_tag="VHM48_04795"
     CDS             complement(3186..6221)
                     /locus_tag="VHM48_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2754755.1"
                     /translation="MTKAARLLGVHPNTVRAWSDAGRLRYYRINPRGDRRYRLGDLQR
                     FLAAAENTPEVGSGPFGHSGHLRRGGGPHERTLDASIASGAATDDDRLRRRADLATVS
                     ALGRIAADPETLDEVLREAMLIIRRRGGFQSTAIYELRGERFVPRAAAPTNRLPDLPR
                     SYGVLGSAVDRAAAGEPGPVEGDGHGGFGDVLAGVSEIAIAIPGDSGPWGVLVILADP
                     DGDPSALDPELLIEVAGAVGSIVEAARRADEVGHRLHRADALRRVASDIGSRLDLDRI
                     LSGLVEHAMVLFNGDRAAVFLVGPDGRTAAEVSRGLSQRYLQSVADFPGRSLPALAAT
                     ARRTLFATHYRDDPRAGDVRASVVQEGFDTICTAPLLDGDVLLGLLNVYHDAPHDWTA
                     DELDTMAALADQAGIAIKNAQTFAKMAGWAAQLQSIQQLGARLSRLGDEHEIGLAIAT
                     ELRQLIDYHNARVYRMRGDDLIPVAMRGQVGEYVDETVEQLAVKYGQGITGWVAEHRV
                     AQNLPDASADPRANTIPGTEDDLDESMLLAPMLYEDQVLGVLVLSKLGLHQFPDDDLR
                     LLVIYASFAAQAFANADATGLLRAQSDALARQLANQRALLQVTESILTTLDPRAILDQ
                     VAERLSDLVGYDNLSIELLDRTSGLLRPLTAKGIHAAEYMEPWTPGEEGLATWVVARN
                     EPTLVLDERTDPRVLQFRLSGPMDGSLICVPLRGRDGATGVLTLERLGINAVYSEEEF
                     ELVKLFAAQVSIALQNAEVHRAVEIRAQTDDLTALLNHGTFQDWLSRSVLAHEPFSLI
                     MLDLDDFKSVNDALGHQAGDRLLAEISRAIEAAGRETDRVFRYGGDEFALILPGTDAA
                     SALGVGERVRAAIHALGEAGTRWADAGMLVSVSIGVATFPRDGLTPEEILLAADRACF
                     VAKRTGLGLIATADEGLAIARDFAFKAPTPIDAIEMSAEAGTAQLSQAAGEPIASDAS
                     GPTSREGSVPVADGHTSTPGPSGARRKPGRPAADASPVP"
     gene            complement(6659..7768)
                     /locus_tag="VHM48_04800"
     CDS             complement(6659..7768)
                     /locus_tag="VHM48_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754756.1"
                     /translation="MTPLVIGAAGLVALVAGGLILRTFGAAYRIGRLLATAPTVTVAE
                     ANAIAAAGRKAYVRVAGRIDADDEFEDADHRPLVFRRVRLETRNGRTWRPFEDHRQSV
                     PFTVNEGLDSIAVDVDALDAGLIVVPRESVGRAADLPDRAPDGIAADTPVRARIDQLS
                     SVEHAIVIGYPVAFAEGPAVVDPSPADDPSPADDPSPGSDPSRAATPGPAGATARMTA
                     GRGRPLILTVLEPPEAMRILAGGDAGRTRLAAGLLGAGAILIVGAFAWAAVTALLPAI
                     VTVIPGAAGWVSSALAATAEPSAATGGDPRSNGQGPGLVGTPGLAIVVVVLIAIGAIV
                     STTIYVRLTTPKSAAPQQPLPGRRDRGAGGDRSRR"
     gene            complement(8026..8655)
                     /locus_tag="VHM48_04805"
     CDS             complement(8026..8655)
                     /locus_tag="VHM48_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754757.1"
                     /translation="MTAGRSSEGGGVGGAASPPRDATIGYPGGLRARWRWAGSGQGAA
                     VFALSEAGGSLEDLGPLVSRDFEQLCRAELRIDGPAGAWTVRMASTIQDVPAGLVWDV
                     ESLFVVKYGFHTYGFESRTGALRWSHRSASPLIAVIGSPRLAHLLVQSEIETFAIEPD
                     GTVGWRIAHSDVVSEAALIGGRLVLTSYTGLVTAIDPATGRATDGHEAI"
     gene            complement(8652..9260)
                     /locus_tag="VHM48_04810"
     CDS             complement(8652..9260)
                     /locus_tag="VHM48_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2754758.1"
                     /translation="MPETIEPAGIQKVRDAAARKGVDLDIRVFDESTHTAADAAATVE
                     AEIGQIVKSLVFVAPAEDGSLEPIICLVSGPNRVDLARLAAVTGERDIRRATAREAQE
                     LTGFVIGGIPPFGHTRSVRTVMDPDLGRFQVVWAAAGTATAVFPVPPGTLRILANAMV
                     APFTEERRAADLEAEAQGQREHDGSSGVGLVGGGATASNAGA"
     gene            9476..10141
                     /locus_tag="VHM48_04815"
     CDS             9476..10141
                     /locus_tag="VHM48_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX2754759.1"
                     /translation="MEAILFDWDGTLVDSLGAFHRANTSVMEAFGLPFDVAQYRIHYT
                     PDWREMYRRLGVPHDRLDEANVLWEATFADDGDKVIPFPGTLLALERLRDAGAVLGIV
                     TAGHRDVVEPQLTRTGLGELLSVRVFGDDLAVHKPDPAPLRLALRLAGDGHRAETSIY
                     VGDAPTDMQMAVAVGARPVGIESVLGDPAELRAAGAVELAPSVAAWAERHLAAVGRGR
                     TAG"
     gene            10146..10829
                     /locus_tag="VHM48_04820"
     CDS             10146..10829
                     /locus_tag="VHM48_04820"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX2754760.1"
                     /translation="MTPATSGHALVLADGSVPAVAQLDEMWLGWSDGLDLVIAADGGA
                     RHAAGLGRPIDLWVGDGDSLGEDEITALAVAGIPIRRSPVDKDESDTELAVLAALDAG
                     ARRVTILGALGGERVDHGLANVWLLGHPRLAGCDVRLIDATVRIRLVGPGLADLGGRI
                     GDLVSLLPFGGDAAGLTTEGLRYPLRDEPLPSGPSRGLSNVRDASQASLAVGSGRILV
                     LETPATLSR"
     gene            10826..11326
                     /locus_tag="VHM48_04825"
     CDS             10826..11326
                     /locus_tag="VHM48_04825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX2754761.1"
                     /translation="MSMPEAGDPAPEIALPDETGTVHRLSEQGGRWTIVYFYPKDDTP
                     GCTVEACEFRDAIETIHERGADVWGISPQGARSKKAFREKFGLPFLLLADEDHAVAEA
                     YGSWVEKMNYGKSFMGVARTTFLIDAEGHVARTWPKVKPEGHAAEVIAALDAARGAGA
                     GAGATR"
     gene            11502..12287
                     /locus_tag="VHM48_04830"
     CDS             11502..12287
                     /locus_tag="VHM48_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX2754762.1"
                     /translation="MNDAPHAWRPDRFMVIAGHPDDADFGPAGTAARWIDEGSTGWLV
                     CCTSGDAGGEDPDADPLDLAALREQEQRDAAAVIGYAGVSFLHMPDGALVNDLALREL
                     LVREIRTFRPDAVLATDPTVIFYRGGGINHTDHREAGLAAVDAVYPAARNPMAFPALA
                     RSGLAAHSVRRLYLFWPTEPNVRIDITATLDRKIAALAAHASQIKEPDRLAARIRAWA
                     AEEGEPIGVAAGEALRMIVIDDDEDEGPGLESAAPAESGSPPT"
     gene            complement(12308..>13117)
                     /locus_tag="VHM48_04835"
     CDS             complement(12308..>13117)
                     /locus_tag="VHM48_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754763.1"
                     /translation="ARHASSARSAEIRAAREAVADCDEARTLEAATARSAAADRPAAD
                     DDGAADVAAAGAAAAGAASRIAAATPEPEDEFAFSTPMNSQAGEDALIVRILRGDREA
                     MQRAVARLAGDDPDARRQWQAQLGGFVEALIARSIEACAFDFPVDHPFWGPFSQSQNR
                     DIAAGLSSLGFRFDGFGDWVDERVPSQRDLSLAVGYAGLDPMRVRRWPTEAETRDLLR
                     DVRVAADEYIAGAAGGLTLGELVTLLGRRADGLTELWNQWGSVRPILLEAT"
BASE COUNT         1873 a         4664 c         4700 g         1880 t
ORIGIN      
        1 gcacgagcgg ggccacgccc tcgccgagca gctccgcggc gccgtcggtc gccgcatcga
       61 ccgcgccaag tgttgccccc agcgtcgccc cgagcgcgac gccggacgcc tgcgccaagg
      121 acaacctcaa gctcacgacc ggcggcaagc tgacgatcgg caccgacaac ccggccttcc
      181 cgccgtacta cgacgccgtc gacagcccga cgaagccgtg gcaactcggc gacccgaccg
      241 acggcaaggg cttcgagagc gcgttcgcct acgccctcgc cgcgaagctc ggattcgaca
      301 aggccgacgt aacctggatc gtcgtcccgt tcgacaactc gttcgcgccg ggcaccaaga
      361 agttcgacct cgacatcaac caggtgtcgt tcaccgccga ccgggccaag gccgtcgaca
      421 tgaccgacgg ctactactcg ctcaaccagt cggtcgtcgc gctcaaggcg aacggcctcg
      481 cgtcggtcac gtcgatcgcg ggcatgaagg gcttcaagtt cggcgcacag atcggcacga
      541 ccagcctcga cacgatcaac aacgtgatcg ccccgacggc cgcggccaag gtctacacga
      601 cgaacgacga cgcgatcgcc ggcttgaagg ccaagcagat cgacgggctc gtcgtcgacc
      661 tcccgaccgc cttctacgtc accgcggtcc aggttgcgaa ctccgtcatc gtcggccagt
      721 tcccggcccc caccggcgcc gacgcggagc acttcagcgt cgtcctggcc aagggcagcc
      781 ccctgacggc gtgcgtcaat acggccatcg ccgccatgaa gagcgacggc agcctcgacg
      841 cgatcacgaa ggagtggctg tccgacaaag ccagtgctcc ggtcctccag ccgtagcggc
      901 cggccgcgtc gcggcctgac atgtcgatca cgggaggccc gctcgacacc ggcgacctga
      961 tcggtctcgc cggtcgacgc gggcctcgcc gttcccggcg ccggatgccg ccgggcgagg
     1021 cggtccggtc gagcctgatc gcgctcctga gcacggtcgt cgtcatcggc ggactggccg
     1081 tcctcgtgac gagctcgccg aactggccgg acgtccggga cgcgttcttc agcgacaaat
     1141 ggttcggctt ctcgtggcca ttgctgatcg acgccttctg gacgaacgtc gcgctgttcc
     1201 ttatcgcgga gctgctgatc ctgccgctgg cgctggtcct ggcgatcgcg cgcggcctgc
     1261 ctgggcccgc gttcttcccg gtccgcctgc tcgcgacgtt ctacgtcgac ttcttccggg
     1321 ccgtcccggg gctcctgatc atcgcgatcc tcgggttcgg gatcccggcc ctcgacctgc
     1381 cgggcgttcc gaacgacgag ttcttctggg cggtgatcgc gctgaccctc gtctactccg
     1441 cctacgtgtc ggaggtctat cgggccggga tcgaatccgt ccattggagc caggaggcgg
     1501 ccgcccgatc gctcggcctc aaccggctgc aggcgcttcg ccacgtcgtc gtcccgcagg
     1561 ccgtccgacg ggtcatcccg cccctcctca acgacttcat cggcctccag aaggacacgg
     1621 tcctcgtgtc gtcgctcggc gtcgtcgaga tcttccggga ggcgtccatc atcaaggcgg
     1681 cgcacttcaa cttcacgccg tatctcgccg tcgctctgat cttcgtcatc ctgacaatcc
     1741 cgctggcgcg cctgacggac tggctggtgt cgcgcgacgc tgcccgtcag gcgagcacgc
     1801 gatgagcatg gcccggcccc gcgaggccct ccggatcgag ggcctccaca agtcgttcgg
     1861 cgacctcgag gtcctccgcg ggatcgacct cgcgatggcc gagcacgagg tcgtctgcct
     1921 gatcgggtcg tcggggagcg gcaagtcgac gctcctgcgc tgcatcaacc tgctcgagcc
     1981 gatcgacgct ggccggatcg tcgtcgaggg tgacgagatc acggccaagg gcgtcgacgt
     2041 caacaggatc cggcggcgga tcgggatcgt cttccaggcg ttcaacctgt tcccgcacat
     2101 gagcgtgctc gccaacgtga cgctcgcgcc gcggaaggtc ctcgggttga gcgcggcgga
     2161 ggcggacgcc cgggcgacgg aactcctcga ccggatcggc ctcgccgaca agcggcgcga
     2221 atatcccgat cgcctgtcgg gtggccagca gcagcgggtc gcgatcgtcc gggcgctggc
     2281 gatgcagccg gacctgctcc tcctcgacga gatcacgagc gccctcgacc cggagctcgt
     2341 ggccgaggtc ctcatcctga tccgcgagct cgccggcaac gggatgacga tgctcatcgc
     2401 gacccacgag atgagcttcg cccgcgagat cgcgaaccgc gtctgcttcc tcgatgccgg
     2461 gcggatcctc gagaccgcgc cgccggccga gatgttcagc gcgccgcgcg aggagcggac
     2521 gaagcagttc ctggagcggg tgatcgcggc cggtcgcctg tgacgatcgc cggcgtcatt
     2581 cggggatcgc ggaatcctcc ggcgcggccg ggtcctcgat ggccgccgcg tcggtcggcg
     2641 ccgcggtggc gtcggtcggg cccgtggcgg ccggatcggg cagatcctgc tcgtcgacga
     2701 catcgttggg cacgaggaac agcgtccagc cgagcttcag gaggttgggc cggtaaccgg
     2761 gcgattcggg gtcgagcgag gggtacgtcg agcggttcca gaacgcgatg ctgcgggcgg
     2821 tcgtcccgaa ccgatgggcg atggtgttga ggctgtcgcc gcttttgacg acgtagacga
     2881 ggaaggtcgg cgcgggcgtc ggggtcgggc ggacgatcgg cgggcgcgcg gtcggcgcgg
     2941 tcgaacctga cggcgaaggc aaggccgtga tggccggttg tccggatcgc ccggaccctg
     3001 ccggccacga gctctcgccg cgcgtcccgc tcggcaggca gccgatcgtg gaaaccgcga
     3061 acaggaccgc gccgacagcg aggatccagc ctcgcccgcg gcgccccatc tccgtggcgg
     3121 gcattggtgg tgcgctgtcg atcgggttca tcatcggccg tgggacgggt cacccgcgcc
     3181 gagcgttacg ggacagggga cgcgtccgcc gccggacggc cgggcttgcg ccgcgctccg
     3241 gacggaccgg gcgtggaggt gtgaccgtcg gcaaccggga ccgatccctc ccggctcgtc
     3301 ggcccggagg cgtcgctcgc gatcggctcg ccggccgcct gcgacaactg agcggtgccc
     3361 gcctcggcgc tcatctcgat cgcgtcgatc ggcgtcggcg ccttgaacgc gaagtcgcgg
     3421 gcgatcgcga gaccctcgtc cgcggtcgcg atgaggccga ggccggtccg tttcgcgacg
     3481 aagcaggcgc ggtcggccgc gagcaggatc tcctcgggcg tcagcccgtc ccgcgggaac
     3541 gtcgcaaccc cgatcgagac gctcaccagc atcccggcat cggcccaccg agtgcccgcc
     3601 tcacccaggg cgtggatcgc ggcgcgcact cgctcaccga ccccgagcgc cgacgccgcg
     3661 tcggtgccgg gcaggatcag ggcgaactcg tcgccgccgt agcggaagac ccggtcggtt
     3721 tcgcgtccgg ccgcctcgat cgcccgcgag atctccgcca ggagccggtc gccggcctgg
     3781 tggccgagag cgtcgttgac gctcttgaag tcatcgaggt cgagcatgat caggctgaac
     3841 ggctcatggg cgagaacgct tcggctcagc cagtcctgga aggtgccgtg attgaggagg
     3901 gcggtgaggt cgtcggtctg ggcccggatc tcgaccgctc ggtggacctc ggcgttctgg
     3961 agcgcgatcg agacctgcgc ggcgaagagc ttgaccagct cgaactcttc ctcgctgtag
     4021 accgcgttga tcccgagccg ctccagggtc agcacaccgg tcgcgccgtc gcgaccacgg
     4081 agcgggacgc agatcaggct gccgtccatc ggtccggaca aacggaactg gaggacgcgg
     4141 ggatcggtcc gctcgtcgag gacgagtgtc ggctcgttgc gggccacgac ccacgtcgcg
     4201 aggccttcct cgccgggcgt ccagggctcc atgtattccg ccgcgtggat gcccttggcg
     4261 gtgagcggtc gcaggagccc gcttgtccga tcgagcaact cgatcgaaag gttatcgtag
     4321 ccgacgaggt ccgacaggcg ctcggcgacc tggtcgagaa tcgctcgcgg gtcgagcgtg
     4381 gtcaggatcg actcggtgac ctggagcagg gcccgctggt tggcgagctg ccgtgcgagt
     4441 gcatcggact gggcccggag gagacccgtc gcgtcggcgt tggcgaacgc ctgggcggcg
     4501 aagctggcat agatgacgag gagtcgcagg tcgtcgtcgg ggaactggtg caatccgagc
     4561 ttggagagga cgagcacgcc gagaacctgg tcctcgtaca gcatcggggc gaggagcatc
     4621 gactcgtcga ggtcgtcctc ggtgccgggg atcgtgttcg cccgcggatc ggcgctggca
     4681 tcgggcagat tctgggcgac gcggtgttcg gcgacccagc cggtgatccc ctggccgtac
     4741 ttgaccgcca gttgctcgac ggtctcgtcg acgtactcgc cgacctggcc ccgcatcgcg
     4801 accgggatca ggtcgtcgcc gcgcatgcga tagacccggg cattgtgata gtcgatgagc
     4861 tggcggagct cggtcgcgat cgccaggccg atctcgtgct cgtcgccgag ccgggacagc
     4921 cgtgcgccga gctgctggat cgactgaagc tgggcggccc agcccgccat cttcgcgaag
     4981 gtctgggcgt tcttgatcgc gatcccggcc tggtcagcga gcgccgccat cgtgtcgagc
     5041 tcgtcggccg tccagtcgtg cggcgcatcg tggtagacgt tcaggaggcc gagcagcacg
     5101 tcgccatcga ggaggggcgc cgtgcagatc gtgtcgaacc cctcctggac gaccgaggcg
     5161 cgcacgtcac cggcccgcgg atcgtcgcgg tagtgggtgg cgaacagcgt ccggcgggca
     5221 gtcgcggcga gcgccgggag ggaccggccc gggaagtccg ccaccgactg gaggtagcgc
     5281 tgcgacaggc cgcggctcac ctcggccgcc gtccggccat ccggcccgac gaggaagact
     5341 gccgcacgat caccgttgaa gaggaccatc gcgtgctcga cgagaccgct caggatgcgg
     5401 tcgaggtcga gccggctgcc gatgtcggac gcaacccggc ggagggcgtc ggcacgatgg
     5461 agccggtgcc cgacctcgtc ggcgcgccgg gccgcttcga cgatcgatcc gacggccccc
     5521 gcgacttcga tgagcaactc cggatcgagc gccgacgggt cgccgtccgg gtcggcgagg
     5581 atgacgagca cgccccacgg gcccgagtcg cccgggatcg cgatcgcgat ctccgacact
     5641 ccggccagga cgtcgccgaa tccgccgtgg ccgtcgccct cgaccgggcc cggttcgccc
     5701 gctgccgccc gatccacggc cgacccgagc accccgtacg agcgcggaag atccggcagc
     5761 cgattggtcg gcgccgcggc gcgcgggacg aagcgctcgc cgcggagctc gtagatggcg
     5821 gtcgattgga aaccgccgcg ccggcggatg atcagcatcg cctcgcgcag gacctcatcg
     5881 agggtctccg ggtcagccgc gatccggccg agggccgaga cggtcgcgag atcggcccgc
     5941 cgacggaggc gatcgtcgtc ggtcgccgcg cccgacgcga tcgatgcgtc caacgtccgt
     6001 tcgtgcggcc caccgccgcg acggaggtgg cccgaatgcc cgaatggccc tgagccgacc
     6061 tcgggggtgt tctccgccgc cgccaggaac cgctgcagat cgccgagccg atagcggcgg
     6121 tccccgcgcg ggttgatccg gtagtagcgc agccggcccg cgtcgctcca ggcccggacc
     6181 gtgttcggat gcacgccgag gaggcgggca gccttggtca ccgagagggt ggcatcgatc
     6241 ggacgggttg gaagcgcgct cggcacgggt cgctcatggg aggaaatgtc aagtcgacgg
     6301 ccgcagccta ccaagctcac agttcttgcc aataggacga aagtcccggt gcttcgatct
     6361 gcgcccgcgg aggctgccga agagccggtc tgtctgcctc agccgcttgt cgttctgacg
     6421 cgcgatccgg gctcgatcga cccgcgtccg gtcgttcgcc gaacgcgccc tcgacccgac
     6481 gttcggcgac gaggatcggg cccggtcggc gttctgagtc gaggacatca cggcccacgc
     6541 cttcacgctc gagcggatcg atcgctggct ggaggcggcc gaccgcgccg ggccgctcgc
     6601 gtccggctga gggcgacggg ctgagggaca cgggccaccg gagatgcccg cgaacggctc
     6661 agcgccggga gcggtctccc ccggctccgc ggtcgcggcg acccgggagg ggctgttgcg
     6721 gggccgcgga cttgggcgtc gtgaggcgga cgtagatggt cgtgctgacg atcgccccga
     6781 tcgcgatcag gacgaccacg acgatggcga gaccgggcgt cccgacgagc ccgggtccct
     6841 ggccgttgct gcgtggatcg ccgccggtgg cggcgctcgg ctcggctgtt gccgcgagcg
     6901 cggagctgac ccaacccgct gcgccgggga tcaccgtcac gatcgccggc agcagggcgg
     6961 tcacggcggc ccaggcgaag gcgccgacga tcaggatcgc cccggctccg agcaacccgg
     7021 ccgcgagccg cgttcgaccg gcgtcgccgc ccgccaggat ccgcattgcc tccggcggtt
     7081 cgaggacggt caggatcaac ggccggcccc ggccggcggt catccgcgcc gtcgcgccgg
     7141 ccggtccggg cgttgccgct cgcgacggat cgctgccagg cgacggatcg tcggcaggcg
     7201 acggatcgtc ggcgggcgac ggatcgacga cggccggccc ctcggcaaat gcgaccggat
     7261 agccgatcac gatcgcgtgc tcgaccgagg acagttggtc gatccgggcg cgcaccggcg
     7321 tatccgcggc gatgccgtcc ggagcgcgat cgggcaggtc ggcggcccgt ccgaccgatt
     7381 cgcgcggaac gacgatcagc ccggcgtcga gggcgtcgac gtcgaccgcg atcgagtcga
     7441 gcccttcgtt cacggtgaac ggcacggact ggcggtggtc ctcgaacggc cgccaggtgc
     7501 gcccgttgcg ggtctcgagg cggacccggc ggaagacgag cgggcgatgg tcggcgtcct
     7561 cgaactcgtc gtcggcgtcg atccggccgg cgacccggac ataggccttg cggcctgccg
     7621 cggcgatggc gttggcctcg gcgaccgtga ccgtcggcgc cgtggcgagg agccgcccga
     7681 tccggtacgc ggcgccgaac gtgcgcagga tcaggccgcc ggcgacgagg gcgaccagac
     7741 ccgcggcacc gatcacgagg ggagtcacgg cccggatgat agccgcccga cgcggcccgg
     7801 ccgtccagcg tctgcccggc cgccgcacga cgggcacgga aagacccgaa ggaacctact
     7861 cgcggcgtgg acgttgatgc gatcgctgcc gttagcctgc gttcagcccc tccgggctgc
     7921 cgtcaacgct agcggtcggc ccgtcggccg gtcaatgcgg ttgtccacca agttccgggc
     7981 ggaatggtcg ggttgtccac cgccgatccc caatccgaag cctggtcaga tggcctcgtg
     8041 gccgtcggtc gcgcggccgg tggcgggatc gatcgcggtc accaaccccg tgtagctcgt
     8101 caggacgagt cgtccgccga tcagcgcggc ctcggacacc acgtcgctat gggcgatccg
     8161 ccagccgacc gtcccgtcgg gctcgatcgc gaaggtctcg atctccgact ggacgaggag
     8221 gtgggcgagc cgcggcgagc cgatgaccgc gatcaacggc gaggccgagc gatgcgacca
     8281 gcggagcgca cccgtccgac tctcgaagcc gtacgtgtgg aagccgtact tgacgacgaa
     8341 caggctttcg acgtcccaga cgagaccggc cggcacgtcc tgaatcgtcg atgccatgcg
     8401 gacggtccag gccccggccg gtccgtcgat ccggagctcc gcccggcaga gctgctcgaa
     8461 gtcgcgggag acgagcggtc cgagatcctc gagggagcca cccgcctccg acagggcgaa
     8521 gactgcggca ccctggccgc tgccggccca gcgccagcgc gcccgcaggc cgccggggta
     8581 gccgatcgtc gcgtcacgtg ggggagaagc cgcgcctccg accccgccac cttcgctcga
     8641 ccggcccgcc gtcacgcccc cgcgttcgag gcggtcgcgc cgccgccgac gaggccgacg
     8701 ccgctcgacc catcgtgctc gcgctgaccc tgggcctcgg cctcgaggtc cgcggcgcgt
     8761 cgctcctcgg tgaacggggc gaccatcgca ttggcgagga tccggagcgt gcccggcggg
     8821 accggaaaga ccgcggtcgc ggtgccggca gcggcccaca cgacctggaa acgcccgaga
     8881 tcgggatcca tcacggtccg gacggaacgg gtatggccga acggtgggat cccgccgatg
     8941 acgaacccgg tcagctcctg cgcctcgcgg gccgtcgccc ggcggatgtc gcgctcgccg
     9001 gtgacggccg ccaggcgggc gaggtcgacc cggttcgggc cggacacgag acagatgatc
     9061 ggctcgagcg atccgtcctc cgcgggggcg acgaagacca gcgatttgac gatctggccg
     9121 atctcggcct cgacggtcgc tgctgcgtcc gcagccgtgt gcgtggattc gtcgaacacg
     9181 cggatatcga ggtcgacgcc cttgcgggcc gcggcgtcgc ggaccttctg gattccagca
     9241 ggttcgatgg tctcgggcac ggccggagtc tacggcatcg ggctcggtgc cgggaatgac
     9301 aggcggggcg gtcgcccggc cccgtccctt gctcgctgct cgcccccctg tcgatatgcc
     9361 ggaccggctc ggcagccggg accggctcgc gatcggaccg gcccggcatt ccgggacccg
     9421 gtggtggcac gcggaccacg gcgccgatcc gccgaggcgc gctagcatcg aacccgtgga
     9481 ggcgatcctc ttcgactggg acggcaccct cgtcgactcg ctcggcgcct tccatcgcgc
     9541 gaacacgagt gtgatggagg cgttcgggct gcccttcgac gtcgcgcagt accggatcca
     9601 ctacaccccg gactggcgcg agatgtatcg ccggctcggc gttccgcacg atcggctcga
     9661 cgaggccaac gtgctctggg aggcgacgtt cgccgacgac ggggacaagg tgatcccgtt
     9721 cccgggcacc cttctggcac tcgagcgatt gcgcgacgcc ggagccgtcc tcgggatcgt
     9781 gaccgccggg caccgcgacg tggtggagcc gcagctcacc cgaaccggtc tgggggagct
     9841 cctgagcgtc cgcgtcttcg gtgacgacct cgcggttcac aagcctgatc ccgcgccgct
     9901 gcgcctggcc ctccgcctgg ccggcgacgg gcaccgcgcg gagacctcga tctacgtcgg
     9961 agacgcgccg accgacatgc agatggccgt cgcggtcggt gcccgtccgg tcgggatcga
    10021 gtcggtcctg ggcgaccccg cggagctgcg cgcggccggc gcggtcgagc tcgccccgtc
    10081 cgtggcggcg tgggccgaac gccacctcgc ggcagtcggc cgcggtcgga cggcgggctg
    10141 acgacgtgac gcccgcgacg tccggccatg cactcgtcct cgccgacggc tccgtgcccg
    10201 cggtggcgca actcgatgag atgtggctgg gctggtccga cgggctcgac ctcgtgatcg
    10261 cggccgacgg cggcgcgcgc catgccgccg gactcggccg cccgatcgat ctgtgggtcg
    10321 gtgacggcga ctcccttggc gaggacgaga tcacggcgct cgccgtcgcg ggtatcccca
    10381 tccggcgctc gccggtcgac aaggatgaat ccgacaccga gctggctgtc ctcgcggcgc
    10441 tcgacgccgg cgcgcggcgc gtcacgatcc tcggcgccct cggcggcgag cgcgtcgatc
    10501 acggccttgc gaacgtctgg ctgctcggcc acccgcgcct cgccggctgc gacgtccgtc
    10561 tcatcgatgc cacggtccgg atccggctcg tcggtcctgg cctggccgat ctcggcggcc
    10621 ggatcggtga cctcgtgtcg ctcctgccgt tcggtggcga cgccgccggc ctgaccaccg
    10681 aaggtctccg ctacccgctc cgcgacgagc cgttgccgag cggcccgtcc cgtggcctgt
    10741 cgaacgtccg cgacgcgagc caggcttcgc tggcggttgg atccggccga atcctcgtcc
    10801 tcgagacccc tgctacgctc tcgcgatgag catgcccgaa gccggtgatc ccgctcccga
    10861 gatcgccctt cccgacgaga ccggcacggt ccaccgcctg tccgagcagg gcgggcgctg
    10921 gacgatcgtg tacttctatc cgaaggacga cacccccggc tgcacggtcg aggcctgcga
    10981 gtttcgcgac gccatcgaga cgattcacga gcgcggtgcc gacgtgtggg ggatcagccc
    11041 gcagggcgcc cggagcaaga aggccttccg cgagaagttc ggtctcccgt tcctcctcct
    11101 cgctgacgag gatcacgcgg tagccgaggc ctacggctcg tgggtcgaga agatgaacta
    11161 cggcaagagc ttcatgggcg tcgcccggac gacgttcctc atcgacgccg agggacatgt
    11221 cgcgcggacc tggccgaagg tcaagccgga aggccatgcg gccgaggtga tcgcggcgct
    11281 cgatgccgcc cggggcgccg gcgcgggcgc gggcgcgacc cgctgagcaa tcgcgtcgca
    11341 tccggatcgc gtccgccggg cggcttcggg tcgctcccga cgcggaaccc cgggcacgag
    11401 ttgaggcttc gctcgtgggg ggagacgagc gaagcctcgg ccgcaatgat agacctgccg
    11461 caaccggcgc aacaaccgga tcacagggcg gaacagccgc gatgaacgac gccccccatg
    11521 cgtggcgccc cgaccggttc atggtgatcg cgggtcatcc cgacgacgcc gacttcgggc
    11581 ctgccggaac ggccgcccga tggatcgatg aaggctccac tggctggctc gtgtgctgca
    11641 cgagcggcga tgccgggggt gaggatccgg atgccgatcc actcgatctc gcggcactgc
    11701 gcgagcagga gcagcgcgac gcggcggcgg tcatcggcta tgcgggcgtg tcgttcctgc
    11761 acatgcccga cggcgcgctc gtcaacgacc tcgccctgcg cgagctgctc gttcgagaga
    11821 tccggacctt ccggcccgac gcggtcctcg ccacggaccc gaccgtgatc ttctaccgcg
    11881 ggggtgggat caatcacacc gaccatcgcg aggcagggct tgccgccgtc gacgcggtgt
    11941 accccgcggc tcgcaacccg atggcgttcc ccgccctcgc ccggtcgggc ctggccgccc
    12001 acagcgtccg ccgtctctac ctgttctggc cgaccgagcc gaacgtccgg atcgacatca
    12061 ccgccaccct cgaccgcaag atcgccgcgc tcgccgccca cgcgagccag atcaaggagc
    12121 ccgatcgact cgcggcgcgg atccgggcgt gggccgccga agagggcgag ccgatcgggg
    12181 tcgcggccgg cgaggcactt cggatgatcg tgatcgacga cgacgaggac gagggcccgg
    12241 gcctcgaaag cgccgcaccg gcggagtcgg gatcgccgcc gacctgagcg gcgcgccgga
    12301 atgcggctca ggtcgcctcg agcaggatcg ggcggaccga gccccactgg ttccagagct
    12361 cggtcaggcc gtcggcccgc cggcccagca gcgtcacgag ctcgccgagg gtcaaccccc
    12421 cggctgcccc ggcgatgtac tcgtcggcgg cgacgcggac gtcgcgcagc aggtcgcgtg
    12481 tctcggcttc ggtcggccag cggcggacac gcatcgggtc gagaccggcg tagccgaccg
    12541 ccagggagag gtcgcgttgg cttggcacgc gttcgtcgac ccaatcgccg aatccgtcga
    12601 accggaaccc gagcgacgac aggccggccg cgatgtcgcg gttctgggat tggctgaacg
    12661 ggccccagaa tgggtgatcg acggggaagt cgaatgcgca cgcctcgatc gagcgcgcga
    12721 tcagggcctc gacgaatccg ccgagctggg cctgccattg ccggcgtgcg tcggggtcgt
    12781 caccggccag ccgggctaca gctcgctgca tggcctcgcg gtcgccgcgg aggatccgca
    12841 cgatcagcgc gtcctcgccc gcctgcgagt tcatcggcgt cgagaaggcg aattcgtcct
    12901 ccggctcggg cgtcgcggcg gcgatccgac tggcggcgcc agcggcggcg gcgccagcgg
    12961 cggccacgtc ggcggcaccg tcatcgtcgg ccgccggccg gtctgcggcc gcggagcgtg
    13021 ccgtggccgc ctcgagcgtc ctcgcctcgt cacagtcggc gacggcctcc cgcgcggccc
    13081 ggatctctgc cgagcgcgcc gacgaggcgt gcctggc
//
LOCUS       DATAIW010000150         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_122521, whole genome shotgun sequence.
ACCESSION   DATAIW010000150 DATAIW010000000
VERSION     DATAIW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122521"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VHM48_04840"
     CDS             complement(<1..1295)
                     /locus_tag="VHM48_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754764.1"
                     /translation="MARDALGSSTEDTEAGRRPRTDLPLGHLARISLYWLGLTAIDGA
                     VGVFTQNRLNYDGFAQPLEVGRFLFFLSIPVAVLSILIQPTVGAISDYTSSRWGRRKP
                     YIVFGSLLDLVFLAGIATANNVLILGVFASFLAISTNIARGPFQGYVPDLISERQVGM
                     ASALVGMMQILGNVTGFLLVTIAVGFHRMEFAIAAVAVVELVTMVAVVVRVSPGPSPL
                     DRAGRSWVTIAGTAWGTDILRERSYVWLVVSRLFFLMGGAMLVNLVITYLKQTHVQSQ
                     EAANGTNTLLLVIVVIANLLAIIPSARISDRIGRKPVIYASCAVGGSGLLIAALAPSI
                     PLLFVGAFLFGASAGTFLAVDWALITDIIPRASSGRYMGLSNVATSSSTVFAVMSGGL
                     VLDFVNSTFGIGTGPRAAYLLGAAYYVVAAICLRPVVEP"
     gene            complement(1385..2098)
                     /locus_tag="VHM48_04845"
     CDS             complement(1385..2098)
                     /locus_tag="VHM48_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX2754765.1"
                     /translation="MCEHFVARASEPFRLDELWPLAERLERYGMAGFGWGAAWLASER
                     RIDTHRDVRAFRDDPLASDLGAVETTSALIHLRRPSKLSTLQLADTQPFFDPGRRFAF
                     SHNGDLRDVRESRRRYQAESRIAGRADSEVGARWLEDAWDTCPSPAAALRSLHATFGG
                     QANLAVITIDGTVAHYAGNTENPAFSFRLGRIGVVSTALYSIDRSLFQLAAPGATERR
                     LVRPGIAVTLDPTGAPLAG"
     gene            2185..2748
                     /locus_tag="VHM48_04850"
     CDS             2185..2748
                     /locus_tag="VHM48_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754766.1"
                     /translation="MDGPLGALAIPIGRGRVTAETASFAALVEGQLDRAFRLATVILG
                     SAIEAEDAVADAALAAWRSRGDLRKTDRFEAWFGRILVNVCRDRLRSRRRRPVTEMPA
                     SEIGGGRLTEAADFRDGVHARDAMSRAFETLEPDERIVLVLRFWHDLTVEAIADRVGI
                     PAGTVKSRLHHATARLRAALAAMEVER"
     gene            2745..3701
                     /locus_tag="VHM48_04855"
     CDS             2745..3701
                     /locus_tag="VHM48_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754767.1"
                     /translation="MIDDATFERDLRAMLAARDPGPAPATLVTIVRFRLETDRRPRRL
                     ASVGRWAGTAAAVGAVAAVLILAVVVARPISISPGSTPLPTPTEPYAIQPGDGVVTGE
                     YLPVAQALVGLIVLGGLAVTFASRRIRSRRIAAALGMLIVAFIATTIGSSNAVAFATG
                     VYGFDPGINPAGSSTGMVVDVEGDQPFTLYLTVTNTSRLPLEILGLADDSVRDQRLPR
                     LVGMGVLSDTSIELSSAPRLPFRPTTLGSGESIDLAILAMSGSCAVHPTPVGGSAVVA
                     PTVVAFGFREVGIVYEQLTIRHWASVTLPETVEIAWPDGPCP"
     gene            complement(3714..4010)
                     /locus_tag="VHM48_04860"
     CDS             complement(3714..4010)
                     /locus_tag="VHM48_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754768.1"
                     /translation="MTVIVVRCEDHRSDGWSCTVTLREDGVPVSTHQVRVRLSDLERL
                     APDASDPTALVKASFAFLLERESPRSILRSFDLLDIARYFPEYEKTIRHTIRAT"
     gene            complement(3985..4803)
                     /locus_tag="VHM48_04865"
     CDS             complement(3985..4803)
                     /locus_tag="VHM48_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2754769.1"
                     /translation="MPEGDTLQRTAMGLAPHLVGRAVTAARVRAGGPQVSRIVGASIT
                     SVEAVGKNLLIRFDNGLELRTHLRMTGSWHRYRPGEPWRRPAARARLVLEVPGAVAVC
                     FDAPVVELFETRAEAIHPTLARLGPDLLDADWSADAAAEARRRLRDPARAAFTISAAL
                     LDQRAVAGIGNIWRNETLFHEGVDPWATVAELDDPTLDRLVATARDLLRRSVDLPTDR
                     SPMGVYRRAGRPCRRCGTLVRSAARDSGVARTTYWCPTCQARNGEVDDRDRRPM"
     gene            complement(4814..>6189)
                     /locus_tag="VHM48_04870"
     CDS             complement(4814..>6189)
                     /locus_tag="VHM48_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754770.1"
                     /translation="SLARLRKEVEPVDPAAFARFLPAWHGVNAVGAAPPPLRQNAALE
                     RLAEVIDQLAGVALPASVLERDILPARVPGYQPRLLDELGALGEVTWAGRGSLGRDDG
                     RIVLVRPGRELLRPSGPVDADARPADLRHEKIREHLAARGASFYRELFAAAGGGSDRE
                     VLDALWDLVWAGEVTNDTFAPLRALRWKRTAHDRSRRPGRLTSLGPPEAAGRWSLVAP
                     PAGSPTERLHATTLAILDRHGIVTREAVAGEAIDGGFSAVYPILRALEDAGRIRRGYF
                     IDGLGAAQFALAGAIERLRSVRDPGRDAADRSVHLLAAADPANPYGAAIAWPRRGEDD
                     RRALQRAAGAYVILVDGVAALYLDRGGGSIQVLPAADDPEVGETAARALAALVDDGRT
                     RELVITKVDGEAVGSSPFRDRLVGAGFVPGYRGVVLRRAVDRAATGRSGASPGRRGAG
                     QLPGPQH"
BASE COUNT          953 a         2137 c         2256 g          843 t
ORIGIN      
        1 tccggctcga cgacgggtcg aaggcagatc gccgccacca cgtaatacgc ggcaccgagg
       61 aggtacgcgg cgcgcggccc ggtcccgatg ccgaaggtgc tgttgacgaa gtccaggacg
      121 aggccaccgg acatcaccgc gaacaccgtc gaagaactgg tcgcgacatt ggacaggccc
      181 atgtaccgac ccgacgaggc gcgcgggatg atgtcggtga tcagggccca gtccacggcc
      241 aggaaggtcc cggccgatgc tccgaacagg aacgcgccga cgaagaggag cggaatcgac
      301 ggggccagcg ccgcgatgag cagcccggac ccgcccacgg cacagctcgc gtagatgacg
      361 ggcttgcgtc cgatgcggtc ggagatccgg gcgctcggga tgatcgccag gaggttggcg
      421 atcacgacga tgaccagcag caacgtgttc gttccgttcg cggcttcctg cgactggacg
      481 tgggtctgct tcaggtaggt gatcacgaga ttcacgagca tcgcgccgcc catcaggaag
      541 aacaggcgcg acacgacgag ccagacatag gagcgttcgc ggaggatgtc cgtgccccag
      601 gccgttccag cgatcgtgac ccacgatcga ccggctcgat cgagcggcga cgggcccggg
      661 ctcacccgca ccacgacggc caccatcgtg accagctcga cgaccgcgac cgcggcgatg
      721 gcgaactcca tccggtgaaa cccgaccgcg atcgtcacca gcaggaaccc ggtgacattg
      781 cccaggatct gcatcatgcc gacgagcgcg cttgccatgc cgacctggcg ctcggagatg
      841 agatcaggaa cgtagccctg gaacgggccg cgggcgatgt tcgtgctgat cgccaggaat
      901 gacgcgaaga ccccaaggat cagcacgttg ttggccgtcg caatgccggc aaggaagacg
      961 aggtcgagca acgaaccgaa cacgatgtac ggcttccgac gaccccaccg gctggaggtg
     1021 tagtcgctga ttgccccgac ggtcggctgg atcaggatcg acaggaccgc gaccgggatc
     1081 gagaggaaga agaggaagcg cccgacctcc agcggctggg cgaacccgtc gtagttcagg
     1141 cggttctggg tgaagacgcc aaccgcgcca tcgatcgccg tcaggccgag ccagtagagc
     1201 gagatcctgg caaggtggcc gagcggaagg tcggtcctgg gccgccggcc cgcctccgtg
     1261 tcctcggtcg atgaaccaag cgcatcccgc gccatctctg ccacgttcca ccctccgctc
     1321 ccccgagcgt gcctccaagt caagcctacg acgccgggcc gagccgattg ttgcgttgtc
     1381 tcgatcagcc ggcgagcggt gcgccagtcg ggtcgagcgt gacggcgatg ccgggccgta
     1441 cgagccgtcg ctccgtggcg cccggcgccg cgagctggaa gagcgaccga tcgatcgagt
     1501 agagcgcggt cgagacgact ccgatccgcc cgagccggaa ggagaaggcc ggattctcgg
     1561 tgttgccggc atagtgggcg accgtgccgt cgatcgtgat caccgcgagg ttggcctgcc
     1621 cgccgaaggt cgcgtggagc gagcggagcg cggcggccgg gctcgggcat gtatcccagg
     1681 cgtcctccag ccagcgcgcc ccgacttcac tgtcggcccg gcccgcgatc cgggattcgg
     1741 cctggtagcg gcggcgcgac tcgcgcacat cgcgaaggtc gccgttgtgg ctgaaggcga
     1801 accgccggcc cgggtcgaag aagggctggg tgtcggcaag ctggagggtc gagagcttcg
     1861 atgggcggcg gagatggatg agcgcgctcg tcgtctcgac ggcaccgagg tccgacgcga
     1921 gcgggtcgtc gcggaatgcg cggacgtcgc ggtgggtatc gatccggcgc tcggaggcca
     1981 gccaggccgc tccccagccg aagccagcca tgccgtaccg ctcgagccgc tcggcaagag
     2041 gccacagctc gtcgagccgg aacggctcgc tggctcgggc gacgaagtgc tcgcacatcg
     2101 gcggcgatga tacgcgtcga cggcgaggcc gtcggttgga tgcgtgaacg ttccgcggcc
     2161 accggtcgtc aggtgcagat gaggatggat ggaccgcttg gagccctggc gatcccgatc
     2221 ggccgcggcc gagtgacggc cgagaccgcg tcctttgccg cgctcgtgga aggccagctc
     2281 gatcgcgcct tccgactcgc gacggtgatc ctcggcagtg cgatcgaagc cgaggacgcg
     2341 gtcgccgacg cggcgcttgc ggcgtggcgg tcgcgcgggg acctgcgcaa gaccgatcgg
     2401 ttcgaagcgt ggttcgggcg gatcctcgtc aacgtctgtc gcgatcggct ccggtcgcgg
     2461 cggcgccgtc cggtcacgga gatgcccgcc tcggagatcg gcggcggcag gctgaccgag
     2521 gctgccgact ttcgcgacgg cgttcatgcc cgcgacgcga tgagccgggc attcgagacg
     2581 ctcgaaccgg acgagcggat cgtcctcgtc ctgcgcttct ggcacgacct gacggtcgag
     2641 gcgatcgcgg accgcgtcgg gatcccggcg ggcaccgtca agtcccgact gcaccacgcg
     2701 accgcccggt tgcgggcggc cctcgcggcg atggaggtcg aacgatgatc gacgacgcga
     2761 ccttcgagcg ggacctccgg gcgatgctgg cggcacggga tccggggccg gcgcccgcga
     2821 ccttggtgac gatcgtccgg ttccggctcg agacggatcg ccggcctcga cgtctcgcgt
     2881 cggtcggccg ctgggccggg acggccgctg cggtcggcgc ggttgccgcc gtgctgatcc
     2941 tcgccgtcgt cgtggcccga ccgatctcca tcagccccgg ttcgacgccc cttccgacgc
     3001 cgaccgagcc gtacgcgatc cagcccggcg acggcgtggt caccggcgaa tatctgccgg
     3061 tcgcccaggc cctcgtcggg ctgatcgtcc ttggaggcct cgctgttacg ttcgccagca
     3121 ggaggatccg atcgcggcgc atcgccgcgg cgctgggcat gttgatcgtc gccttcatcg
     3181 ccacgacgat cggatcgtcg aatgcggtcg cgttcgcgac cggggtgtac ggcttcgatc
     3241 cgggaatcaa cccggccggt tccagcacgg gaatggtcgt cgacgtcgag ggcgaccagc
     3301 cgttcaccct gtacttgacg gtgacgaaca cgtcccggct tccgctcgag atcctgggcc
     3361 tggccgatga ctcggtccgg gatcagcgtc tgccgcggct cgtcggcatg ggcgtgctgt
     3421 ccgacacatc gatcgagctc tcgtcggcgc ctcggcttcc gttccgaccc acgaccctcg
     3481 gatccggcga gtcgatcgac ctcgccatcc tggccatgtc gggctcgtgc gccgtgcacc
     3541 cgaccccggt cggtggatca gctgtggtcg cgccgaccgt tgttgcattc gggttccggg
     3601 aggtcgggat cgtgtacgaa cagctgacga tccgccattg ggcgtcggtc accctccccg
     3661 aaaccgtcga gatcgcctgg ccggacgggc cgtgcccgtg aggcgtccga gggtcaggtc
     3721 gcgcggatgg tgtggcggat cgtcttctcg tactcgggga agtaccgcgc gatatcgagc
     3781 aggtcgaacg agcgcaggat cgaccgcggc gactcgcgct cgaggaggaa tgcgaacgac
     3841 gccttgacga gcgccgttgg gtcggaggca tccggcgcga ggcgttcgag gtcggacagg
     3901 cgcacccgaa cctgatgggt cgagaccggg actccgtcct cgcggagcgt caccgtgcac
     3961 gaccagccgt ccgagcgatg gtcttcacat cggacgacga tcacggtcat cgacctctcc
     4021 gttcctggcc tgacaagtcg ggcaccagta cgtcgtccgc gcgaccccgc tgtcccgcgc
     4081 tgccgacctg acgagcgtgc cacagcgacg gcacggtcga ccagcccgac ggtacacccc
     4141 catcggtgac ctgtccgtgg ggagatcgac gctccgtcgg agcaggtcgc gggccgtcgc
     4201 gaccagccgg tcgagggtcg ggtcatcgag ctcggcaacc gtcgcccatg gatcgactcc
     4261 ctcgtggaac agagtctcgt tgcgccagat gttcccgatc ccggcaacgg cccgctgatc
     4321 caggagcgcc gccgagatcg tgaatgccgc gcgggcgggg tcgcgcaggc ggcggcgcgc
     4381 ctcggcggcc gcgtcggcac tccagtcggc gtcgaggaga tccgggccca ggcgggccag
     4441 cgtcgggtgg atcgcctcgg cccgcgtctc gaaaagctcg acgaccggcg cgtcgaagca
     4501 cacggcgaca gcgcccggga cctcgaggac gagccgggcc cgggcggcgg ggcgacgcca
     4561 cggttcgccg ggtcggtagc ggtgccagga cccggtcatc cggaggtggg ttcgcagctc
     4621 gaggccgttg tcgaatcgga tcagcaggtt cttgccgacc gcctcgacgc tcgtgatcga
     4681 cgcaccgacg atccgcgaga cctgggggcc accggcgcgg acacgggccg ccgtgaccgc
     4741 ccgaccgacg aggtgcggcg cgagtcccat cgccgttcgc tggagggtgt cgccctcggg
     4801 catcgacggg ccgtcagtgc tgcgggccgg ggagctggcc ggcgccacgg cgaccgggcg
     4861 acgctccgga gcggccggtc gcggcgcgat cgacggcccg gcgcaggacg acaccgcgat
     4921 agcccgggac gaagccggca ccgacaagtc ggtcgcggaa cggggacgac ccgaccgcct
     4981 caccgtcgac cttcgtgatc acgagctcgc gcgtccgacc gtcgtcgacg agcgcggcga
     5041 gcgcccgcgc cgccgtctcg ccgacctcgg gatcgtcggc cgctggcagg acctggatcg
     5101 agccgccgcc gcggtcgagg tagagggcgg caacgccgtc gaccaggatc acgtaggctc
     5161 cagccgcgcg ctgcagtgcg cgtcgatcat cctcgccgcg ccgcggccag gcgatcgcgg
     5221 cgccgtacgg gttggccgga tccgcggcgg cgaggagatg gaccgatcga tctgcggcgt
     5281 cgcggcccgg atcgcgcacg gagcgcaacc gctcgatcgc ccccgcgagc gcgaactggg
     5341 cggccccgag gccgtcgatg aagtaaccgc gccggatccg cccggcatcc tcgagggccc
     5401 gcaggatggg atagaccgcc gagaacccgc cgtcgatcgc ctcgcccgcg accgcctcgc
     5461 gggtcacgat cccgtgccga tcgaggatgg ccagcgtcgt cgcgtggagc cgctcggtcg
     5521 gcgaaccggc cggcggcgcg acgagcgacc agcggccggc cgcctcgggc ggacccagcg
     5581 acgtcaaccg accggggcgg cggctccggt cgtgcgccgt ccgcttccaa cggagagccc
     5641 ggagcggggc gaacgtgtca ttcgtgacct cgccggccca gaccaggtcc cagagcgcat
     5701 cgaggacctc gcggtcggat ccgccccccg ccgcggcgaa cagctcacgg tagaaggagg
     5761 cgcctcgggc ggcgaggtgc tcgcggatct tctcgtgccg cagatcggcc ggccgggcat
     5821 ccgcgtccac gggcccggac gggcggagca gctcacgacc cggccggacg aggacgatcc
     5881 ggccgtcgtc gcgaccgagg ctcccccgcc ccgcccaggt cacctcgccc agggcaccca
     5941 gctcgtcgag gaggcgcggc tggtagcccg ggacgcgggc cggcaggatg tcgcgttcga
     6001 ggaccgatgc gggcagcgcc acgccggcga gctggtcgat cacctcggcg aggcgctcca
     6061 gagccgcgtt ctggcggagt ggcggcggcg cagccccgac cgcgttcacg ccgtgccagg
     6121 ccggcaggaa ccgggcgaag gcggcggggt cgaccggctc gacctccttg cggaggcggg
     6181 ccagggagc
//
LOCUS       DATAIW010000151         4000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211859, whole genome shotgun sequence.
ACCESSION   DATAIW010000151 DATAIW010000000
VERSION     DATAIW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211859"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VHM48_04875"
     CDS             complement(<1..1276)
                     /locus_tag="VHM48_04875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX2754771.1"
                     /translation="MAIAPRADADGLLEDLNAAQREAVTATRGPVAIVAGAGTGKTRV
                     ISRRTAYAIATDVVPADQALVVTFTDKAAREMVERLAGLGLPAVVARTFHAHALSQLR
                     FFWPSRHDGTPLPALLDSKIPLLVRAARGLPPPWKFTPIRDLATEIEWAKSRGIEPAA
                     YEAGLGTREPPIRADLMARVFADYERAKTRDGRIDFDDLLARTISLLETDVEAAGIVR
                     ARKSWFSVDEYQDTNPHQQRLLELWAGDRDDICVVGDEDQTIYTFTGATSAYLTGFAA
                     RHAGARVVALTENYRSTPQILGLANRLLTAEGRSKALTATRPDGSPPTIEGYPDGAME
                     LAAMTRTIRGLLAGGGADRVEAAEVAILVRMNAQMAPIEEALTRAGIAYQVRGVRFYD
                     RPEVAAALAMIRRLTTAATGRPLATAIRGAFRD"
     gene            complement(1290..1586)
                     /locus_tag="VHM48_04880"
     CDS             complement(1290..1586)
                     /locus_tag="VHM48_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754772.1"
                     /translation="MRPTVASINLSASTGRAGLKVGDRVRINGTGLYSGELALIEKLT
                     TGAIPSAVVRTEAGRTRQVRTIDLEPVAAPPPAAPPPAAPPSPATGSGPDASEG"
     gene            complement(1913..2353)
                     /locus_tag="VHM48_04885"
     CDS             complement(1913..2353)
                     /locus_tag="VHM48_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2754773.1"
                     /translation="MIEPIRLAFEVACPPDHAFDVWTADIDRWWPADHTVGGTDDLTV
                     VLEPRPGGRIFERSPGGIEHDWGEVTIWDPPARFGYLWHLRRDRADATEVEIRFVAAG
                     EGSTRVEIEHRGWEALGAGGETWRDRNHGGWATLLPWFLAAAEA"
     gene            complement(2358..2756)
                     /locus_tag="VHM48_04890"
     CDS             complement(2358..2756)
                     /locus_tag="VHM48_04890"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2754774.1"
                     /translation="MTDRRAAARPMEAAGPAGDPFEALGDPNRRAIVELLGAGLPDGC
                     SVGDLARVLPISRPAVSRHLRLLKAAGLVVEEPRGTRRLYRLHDQGLEAVRAYLDRVW
                     GDAATRFRLVAENARSDDAGPGATGRRPVR"
     gene            complement(2761..3168)
                     /locus_tag="VHM48_04895"
     CDS             complement(2761..3168)
                     /locus_tag="VHM48_04895"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754775.1"
                     /translation="MANGSGTKADPWVLKTAPGTSDYTMYRDDAADPPQIVCMVGSTK
                     LTYDARAIDELHAMLEAHGDWMPLGGADEQKPAPDGSVEAWGRSESNPRGGWYGLRKG
                     YRGRFGVYMPPLLEALGLVELTHDAKNNRVRAT"
     gene            3330..3641
                     /locus_tag="VHM48_04900"
     CDS             3330..3641
                     /locus_tag="VHM48_04900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754776.1"
                     /translation="MTEPSAVAADLVVEDNPDESRFEAWLGDRMVGIAEYELPDETGP
                     IVFVHTEVLPGAEGMGVGRGLARGALDDVRRRGLRLVAECEFIAGFLKRHPEDRDLVA
                     D"
     gene            complement(3664..>4000)
                     /locus_tag="VHM48_04905"
     CDS             complement(3664..>4000)
                     /locus_tag="VHM48_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754777.1"
                     /translation="PTTLLKIWTGNPVIWSMAAMAVAVIGRSRFAAPFVLLKPSLAPF
                     ALFGVRRRSWWVGLALVVAICLPFGALWADWVASVLNSRGGGLLYSALEIPILLLPLV
                     AWLGRTRGG"
BASE COUNT          567 a         1444 c         1428 g          561 t
ORIGIN      
        1 catcacggaa ggcgccacgg atcgccgtcg ccagcggccg accggtcgcc gcggtcgtga
       61 gccggcggat catcgcgagc gcggcggcga cctccggccg gtcgtagaac cggacgccgc
      121 ggacctggta ggcgatcccg gcgcgcgtca gggcctcctc gatcggggcc atctgggcgt
      181 tcatccggac gaggatggca acctcggcgg cctcgacccg gtccgctccg ccgcccgcca
      241 gcaggccgcg gatcgtgcgg gtcatcgctg ccagctccat cgccccgtcg gggtagccct
      301 cgatcgtcgg cggagatcca tcgggacgag tcgccgtcaa cgccttcgat cggccctcgg
      361 cagtcaggag gcggttggcg agacccagga tctgcggggt cgagcggtag ttctccgtca
      421 gggcgacgac tctggcgccg gcgtgccggg cggcgaaccc ggtcaggtag gcagaggtcg
      481 caccggtgaa cgtgtagatc gtctggtcct cgtcaccgac gacgcagatg tcgtcgcggt
      541 cgcccgccca cagctcgaga aggcgttgct ggtgggggtt cgtgtcctgg tattcgtcga
      601 cgctgaacca cgacttccgg gcgcggacga tcccggccgc ctcgacgtcg gtctcgagga
      661 ggctgatcgt cctggcaagg agatcgtcga agtcgatccg gccgtcgcgc gtcttggccc
      721 gttcgtagtc ggcgaagacg cgagccatca ggtcggcccg gatcggcggc tcgcgggtcc
      781 cgaggccagc ctcgtacgcc gccggttcga ttccgcgcga cttcgcccac tcgatctcgg
      841 tcgcgaggtc gcggatcggg gtgaacttcc agggcggagg cagaccccgg gcagcccgca
      901 ccaggagcgg gatcttcgag tcgaggaggg caggcagggg cgtcccatcg tgtcttgacg
      961 gccagaagaa ccggagctgc gacagcgcat gagcgtggaa cgtccgggca acgaccgccg
     1021 gcagaccgag cccagccagc cgctcgacca tctcgcgggc ggccttgtcg gtgaacgtca
     1081 caacgagggc ctggtcggcc gggacgacgt cggtcgcgat cgcgtacgcc gtccttcggc
     1141 tgatcacccg ggtcttgccc gtccccgcac cggcgacgat cgcgaccggg ccgcgcgtgg
     1201 cggtgacagc ttctcgctgg gccgcgttca ggtcctcgag cagcccgtcg gcgtcggccc
     1261 gcggtgcgat ggccacgtcc gacccccgat cagccctcgc tcgcgtcggg cccgctgccc
     1321 gtcgccggcg agggcggcgc ggccggcggt ggcgcggccg gcgggggagc cgcgacgggc
     1381 tcgagatcga tggtccggac ctgacgcgtc cgacccgcct cggtcctgac gaccgccgat
     1441 gggatcgccc cggtcgtgag cttctcgatc aacgcgagct cgcccgagta cagaccagtg
     1501 ccgttgatcc ggacccgatc gccgaccttc aggccggccc gtccggtgct cgccgacagg
     1561 ttgatcgagg cgaccgtcgg ccgcacgtac ggctcgccaa tcgggggacc gcccggacct
     1621 cccggctcgc ccggtcgtgg cgggcgtgtg gcgttgcggg gcggtgggta gacggtccgg
     1681 cgcttgcgtg gcggcactgg ctgagcggtc ctcctggttc ggtgacgggc ggcgatcggt
     1741 ggcgccacag gacgcggcgt tcgatccgtt caggtgcagc atgccacacc gcgatcaccc
     1801 ggccattggt cggacccccg aaccgcctgc acgctggctc atcggccgat catccgccag
     1861 ccggctcgag cgtggtcgag ggcggtcgat ctcggacggg atcgcccggt cgctacgcct
     1921 cggccgccgc caagaaccac ggcagcaggg ttgcccagcc gccatggttg cggtcgcgcc
     1981 acgtctcgcc cccggcaccg agggcctccc agccgcgatg ctcgatctcg acccgtgtgc
     2041 tcccctcccc cgcggcgacg aaccggatct cgacctcggt cgcgtccgcg cggtcgcgtc
     2101 ggaggtgcca caggtagccg aaccgggcgg gcggatccca gatcgtgacc tcgccccaat
     2161 cgtgctcgat cccgcccggc gaccgctcga agatccggcc gcccggccgt ggctcgagga
     2221 cgacggtcag gtcgtcggtc ccgccgaccg tgtggtcggc gggccaccat cgatcgatgt
     2281 cggccgtcca gacatcgaac gcgtggtccg gcgggcaggc aacctcgaac gcgaggcgga
     2341 tgggctcgat catcgggcta tcggaccggc cgtcggccgg tcgcgccggg tccagcgtca
     2401 tcggatcggg cgttctcggc gaccagccgg aaccgcgtcg cggcatcgcc ccagacccga
     2461 tcgagatagg cccggacggc ctcgaggccc tggtcgtgga ggcgatagag gcggcgcgtc
     2521 ccgcgcggtt cctcgacgac cagtccggcg gctttcagga ggcgcaggtg gcgcgacacg
     2581 gccggtcggc tgatcggcaa taccctcgcg aggtcgccga cggagcagcc atccggcaag
     2641 cccgccccga gcagctccac gatcgcgcgc cggttcgggt cgccgagcgc ctcgaacgga
     2701 tcacccgccg gcccggcagc ctccatcggt cgcgcggctg cgcgccggtc ggtcatcgac
     2761 tcacgtcgcc cggacccggt tgttcttcgc atcgtgggtc agctcgacga gcccgagcgc
     2821 ctcgagcagc ggcggcatgt acaccccgaa ccggccgcga tagcccttcc tgaggccgta
     2881 ccagccgccc cgcgggttgg attcggagcg accccaggcc tcgaccgagc cgtcgggcgc
     2941 gggcttctgc tcgtcggcgc cgccgagcgg catccagtcg ccgtgggcct cgagcatcgc
     3001 gtggagctcg tcgatcgccc gggcgtcata ggtcagcttc gtcgagccga ccatgcagac
     3061 gatctggggc gggtccgcgg cgtcgtcgcg atacatcgtg tagtcggacg tgcccggagc
     3121 cgtcttcagg acccatggat cggccttcgt gccgctgccg ttcgccatcg tggatctccc
     3181 tcgcagactc ggacgggccg atcgaatcgc gcatcgcgcc gtcggtaatc agatggttac
     3241 cgtaaccgat cggtcaccga tgccgcaagg ccgggatcga tgccgaccga ccgggccggg
     3301 ttccgctccg acggtctagg atgcaacgaa tgaccgagcc gtccgccgtc gccgccgacc
     3361 tcgtcgtcga ggacaacccc gacgaaagcc gcttcgaagc ctggctcggc gaccggatgg
     3421 tcgggatcgc cgagtacgag ctgcccgacg agacggggcc gatcgtgttc gtccacaccg
     3481 aggtcctgcc cggtgcggaa ggcatgggcg tcgggcgcgg gctggcccgc ggggcgctcg
     3541 acgacgtccg gcgccgcggg ctccgcctcg tcgccgaatg cgagttcatc gccggcttcc
     3601 tgaagcgcca tcccgaggat cgcgacctcg tcgccgactg acggaccgac gcgtcgcggt
     3661 cgcctacccg ccgcgcgtcc gaccgagcca ggccacgagc ggcagcagca agatcgggat
     3721 ctcgagggcc gagtagagca ggccgccgcc gcgcgagttc aggacggacg cgacccagtc
     3781 cgcccagagc gcaccgaatg gcaggcagat ggcgacgacc aaagccagcc ccacccacca
     3841 cgacctccgc cggaccccga acaaggcgaa cggcgcgagc gacggcttga gcaggacgaa
     3901 gggtgcggcg aaccgcgaac ggccgatgac cgccacggcc atcgccgcca tcgaccagat
     3961 gaccgggttg cccgtccaga tcttgagcag cgtcgtcggg
//
LOCUS       DATAIW010000152         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_241827, whole genome shotgun sequence.
ACCESSION   DATAIW010000152 DATAIW010000000
VERSION     DATAIW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241827"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VHM48_04910"
     CDS             complement(<1..685)
                     /locus_tag="VHM48_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754778.1"
                     /translation="MNALLLLTVANIKSFTRDRAALFWTLAFPLIFVVLFGSIFSGGN
                     NQRTIGFADLDGTPASATLSAAFGAVDGVKLVDGSEADLVARMKKGEVSAVIVVPKGY
                     AETVGAKAAPAAVTVYTDPSQSATDGATRQLVGFVLSAVNQAATGQPPAVLPTFQPVQ
                     TNDLTFISYLVPSILGMSLMQLGVFAAIPLVADRQKLILKRLQATPLRRWQLVGSNVL
                     MRLLIAIFQT"
     gene            complement(696..1634)
                     /locus_tag="VHM48_04915"
     CDS             complement(696..1634)
                     /locus_tag="VHM48_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754779.1"
                     /translation="MTTPSTAADPVIRVSGLHKRYGETHAVDGVSFEVPAGTVFGLLG
                     PNGAGKTTTVEVLEGLRIPDSGEVRVLGIDAVHHPDDLKPRIGVSLQTAALYPKLTVV
                     EVLDLFRSFYPKGRPTGDLVEMMDLGEKRSTRTQDLSGGQRQRLSVALALVNDPELVF
                     LDEPTTGMDPAARRALWEVVLGLKAEGRTVLLTTHYMEEAEILCDRLAIMDHGHILEE
                     GTVGELISRRFKERAVRFDRIDGLADDRMAAMAGVSSVKHENGEVLLYTRDVPATIGA
                     VLDASEELGVEPANLGVRRATLEDVFLDLTGRALRD"
BASE COUNT          262 a          604 c          608 g          263 t
ORIGIN      
        1 ccgtctggaa gatcgcgatc aggagccgca tcaggacgtt gctcccgacg agctgccagc
       61 gccggagcgg cgtcgcctgg aggcgcttga ggatcagctt ctggcgatcg gcgacgagcg
      121 ggatggcggc gaacaccccg agctgcatca gcgacatccc gaggatcgag gggaccaggt
      181 aggagatgaa cgtcaggtcg ttggtctgga ccggctggaa cgtcggcagg acggccggcg
      241 gttgcccggt ggccgcctgg ttgacggccg acaggacgaa cccgaccagc tggcgcgtcg
      301 cgccgtccgt cgcgctctgg ctcgggtccg tgtagacggt gaccgcggcc ggggcggcct
      361 tggcgccgac ggtctccgca tagcccttcg ggacgacgat gaccgccgac acctcgccct
      421 tcttcatccg ggcgacgagg tccgcctcgg agccgtcgac gagcttcacg ccgtcgaccg
      481 cgccgaaagc ggccgacagc gtggccgacg ccggcgtgcc gtccaggtcg gcgaacccga
      541 tcgtccgctg gttgttgccg cccgagaaga tcgagccgaa cagcacgacg aagatcagcg
      601 gaaaggcgag cgtccagaag agtgccgccc ggtcacgggt gaagctcttg atgttggcga
      661 cggtcagcag gagcagcgcg ttcatctcgt ccccctcagt cccgcaacgc ccgaccggtc
      721 aggtcgagga acacgtcctc gagcgtcgcc cgccggacgc cgaggttggc cggctcgacg
      781 ccgagctcct cactcgcgtc gaggaccgcg ccgatggtcg ccggcacgtc ccgggtgtag
      841 aggaggacct cgccattctc gtgcttgacc gacgagaccc cggccatcgc cgccatccgg
      901 tcgtcggcga ggccgtcgat ccggtcgaac cggaccgccc gctccttgaa ccgccggctg
      961 atcagctcgc cgaccgttcc ttcctcgagg atgtggccgt ggtccatgat cgcgaggcgg
     1021 tcgcacagga tctccgcctc ctccatgtag tgggtcgtca gcaggacggt ccggccctcg
     1081 gctttcaggc cgaggacgac ctcccacagc gcccgccgcg cggccgggtc catgccggtc
     1141 gtcggctcgt cgaggaagac cagctccggg tcgttgacga gggcgagcgc gaccgacagc
     1201 cgctggcgct ggccgcccga gaggtcctgg gtccgggtcg agcgcttctc gccgaggtcc
     1261 atcatctcga cgaggtcgcc ggtcgggcgg cccttcggat agaagctccg gaagaggtcg
     1321 aggacctcga ccaccgtcag cttcggatag agagcggccg tctggagcga cacgccgatc
     1381 cgcggcttga ggtcgtcggg gtgatggacg gcgtcgatcc cgaggactcg cacctcgccc
     1441 gaatcgggga tcctgagacc ctcgagaacc tcgacggtcg tcgtcttgcc cgcgccgttc
     1501 ggcccgagca acccgaacac ggtccccgcc gggacctcga agctcacccc gtcgacggcg
     1561 tgcgtctcgc cgtatcgctt gtgcaggccg gagacgcgga tgacgggatc cgcggcggtc
     1621 gatggcgtcg tcacgtcccc gatggtgcca ttcccggccc cgatccgccg tccgcattcc
     1681 cgtccgtggg atcgccgtcg acgtcgaatg ggtcgagttc gagcgggtcg acgtcga
//
LOCUS       DATAIW010000153         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_97227, whole genome shotgun sequence.
ACCESSION   DATAIW010000153 DATAIW010000000
VERSION     DATAIW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97227"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1476)
                     /locus_tag="VHM48_04920"
     CDS             complement(241..1476)
                     /locus_tag="VHM48_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2754780.1"
                     /translation="MTVRRVPIGQRSETVRRANLSAIVGELHSRGPQSRSELVARTGL
                     TRSAIRGLIGELTKAGLVGEERAVPLGNPGRPSPLVRPEPEGVVVLALEIAVDSLAVA
                     LVGLGGNVLASIRVDRPRDHVSVDAIVADLVGLADQVLVPSRRRSAVIGVGVAVAGVV
                     RRTDGFVSMAPNLGWRDVALGERLTAGLGLAVPLEVANEADLGALAEVRRGAARGADH
                     VIYLSGEVGVGGGIIVDGRPLLGVAGYGGEIGHLPINPAGLRCACGSIGCWETEVGAS
                     ALLRRAGRATDGGRSEVTALLAAAAAGDPVALAALEETGDWLGLGLAGLVNILNPGLV
                     VFGGLFEHLYPFIERRVRATLDDRALRAPRELVGLAPSALGDDAPLLGAAELAFEPFL
                     ADPARWLQPRDSLLERASA"
     gene            1607..2593
                     /locus_tag="VHM48_04925"
     CDS             1607..2593
                     /locus_tag="VHM48_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2754781.1"
                     /translation="MTRLQWGIIGPGRIAPRVARALVDNPRGELRAVASRDLERARGF
                     ANAHGAAVAHGSYADLIADPAIDVVYIALPNHLHAEWSIRALDAGKHVLCEKPVALTV
                     ADVDEIAAAAARNGRIAVEGFMYLHHPQTLAALDIVASGALGPLQLIAASFAFLLTAP
                     DDPRLDPAMGGGSLWDVGCYPVSIARRIAGREPDGIAGFARFDERGVDRTFVGQLRFK
                     GGLLASFDSGFASPDRERVEVVGGAAILVIEHPFLPEPYGPPPVVTVTRDGVASALGV
                     ASVDEYRAEVDDLHAAILDGTRPRVDLAFSRGSIATLVELDRVARIGEGSGG"
     gene            2689..>3617
                     /locus_tag="VHM48_04930"
     CDS             2689..>3617
                     /locus_tag="VHM48_04930"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754782.1"
                     /translation="MAIVVAAGTFGTLAVDRVTTADRCPPTTAHADWSVARRWDEALL
                     DAIRRALPNPPVHARNLFHTSVAMWDAWATYDPTASGDLFTEKHTTLDVAAARNEAIS
                     YAAYRVLSARYIKAVGGSESLNEFDDLMDTLCYPIADTGTTGNEPAAVGNRIAAAVLA
                     AGRADGSNEAGGYAAPDYKSVNPPLVVNQTGTTLVDPNRWQPLQLEHMISQNGIPVTN
                     GVQQAIGPHWGHVTSFALPTGGAAGTPIDPGPPPQLGNPATDQAYKDQAVEVIRDSSE
                     LDAAAGVMIDVSPGARGGNTIGTNDGSGHPVDPA"
BASE COUNT          559 a         1326 c         1256 g          476 t
ORIGIN      
        1 ccggtggccg agccgccacc gccacccgac ggagcaggcg tggcggtcga cccgcacgca
       61 ctcaggacca tggcactcgt cgccgcgacg gcggccagct tccgactgaa cttcatccgg
      121 ttcttcctcc tctggacatc ccgctcatct tgacggtcga tatcccgcgc taggctactc
      181 ccctcctcac gccgcatgtg gcgaaactgc gctccgtgaa tgcggttcga ccggaaacgc
      241 tcatgccgaa gcccgctcga gcagggagtc tcgtggctgg agccagcggg cggggtcggc
      301 aaggaagggc tcgaacgcca gctccgccgc acccagcagc ggtgcgtcgt cgccgagcgc
      361 ggacggcgcg aggccgacga gctcgcgcgg cgcgcgcagg gcgcgatcgt cgagggtcgc
      421 ccgaacccgt cgctcaatga acggatagag gtgctcgaac aggccgccga agacgaccag
      481 acccgggttg aggatgttga cgaggccggc caggccgagc ccgagccagt cgcctgtttc
      541 ctcgagcgcc gcgagtgcga ccggatcgcc cgcggcagcc gcggcgagga gggctgtcac
      601 ctccgaccgg ccgccatcgg tcgcgcgccc ggcgcggcgc aacagggcgc tcgcgccaac
      661 ctctgtctcc cagcagccga tcgaaccgca ggcacaccgc agtccggccg ggttgatcgg
      721 caggtgcccg atctcgccgc catagccggc aacacccagc agcggtcgac cgtcgacgat
      781 gatcccgccg ccgacaccga cctcaccgga caggtagatg acgtggtcgg cgccgcgagc
      841 cgcaccccgc cggacctcgg cgagggcccc gaggtcggcc tcgttggcaa cctcgagggg
      901 gaccgccagg ccaaggcccg cggtgagtcg ctcgcccagg gcgacgtccc gccagccgag
      961 gttcggggcc atcgagacga acccatccgt ccgccggacg acacccgcga cggcgacacc
     1021 cactccgatc acagcgctcc gccgacgact cgggacgagg acctggtcgg cgaggccgac
     1081 gaggtcggca acgatcgcat cgacggacac gtgatcgcgt ggccgatcca cccggatcga
     1141 ggccaggacg ttgccgccga ggccgaccag ggcgacggcc agcgagtcga cggcgatctc
     1201 cagggccagg accacgacgc cttccggctc aggccgaacg agcggagacg ggcggcccgg
     1261 gttgccgagg ggaacggcgc gctcctcacc gacgagcccc gccttcgtca attcgccgat
     1321 gaggccgcgg atcgcgctcc gggtcaggcc ggtccgggcg acgagctcgg agcgggactg
     1381 cggaccgcgc gaatgcagct cgccgacgat cgcgctgagg ttggcgcgcc ggaccgtctc
     1441 actgcgctgg ccgatcggaa cccgtcggac cgtcacctga tttgtgatcc tcttgcccaa
     1501 atgtttgtga taccttgtta catgacccgt tgccctgtca agcgcgccga gcgcgccgga
     1561 gtcgggacgg ccatccgccc ccatcgatga agaggcgctc ctgcggatga cccggttgca
     1621 gtggggaatc atcgggcccg ggcggatcgc cccgcgcgtg gcacgggcgc tggtcgacaa
     1681 cccacgcggc gagctgcgcg cggtcgccag ccgcgacctc gagcgagccc gtgggttcgc
     1741 gaacgcgcac ggggcggcag tggcccacgg ctcgtacgcg gatctgatcg ccgacccggc
     1801 gatcgacgtg gtctacatcg ccctgccgaa ccacctccac gccgagtggt cgatccgggc
     1861 gctcgatgcc ggcaagcacg tcctctgcga gaagccggtg gccctgaccg tcgccgacgt
     1921 cgacgagatc gcggcggcgg ccgcacggaa cggacggatc gcggtcgagg ggttcatgta
     1981 cctccatcat ccgcagacgc tggccgcgct cgatatcgtc gcctccgggg cgctcgggcc
     2041 gctgcagttg atcgccgcct cgttcgcctt cctcctgacg gcccccgacg acccgcgcct
     2101 cgaccccgcg atgggtggcg gctcgctctg ggacgtcggc tgctatccgg tgagcatcgc
     2161 ccgacggatc gccggccgcg agccggacgg gatcgccggg ttcgcccggt tcgacgagcg
     2221 cggcgtcgac cggaccttcg tcggccagct ccggttcaag ggtggactgc tcgcctcgtt
     2281 cgacagtggc ttcgcctcgc ccgaccggga gcgggtggaa gtcgtcgggg gggccgcgat
     2341 cctggtcatc gagcatccgt tcctgccgga accgtacggg ccgccgccgg tcgtgaccgt
     2401 cacccgcgac ggcgtcgcgt ccgcgctcgg tgtcgcgtcc gtcgacgagt accgagcgga
     2461 agtcgacgat ctccacgcgg cgatcctcga cggcacgcga ccacgggtcg acctggcctt
     2521 cagccggggt agcatcgcga cactcgtgga actcgatcga gtcgcccgga tcggagaggg
     2581 gtcgggcggc tgaccggtta cggcgagagc gggccgtggg gggcgaacgc gcccggcaac
     2641 tggaggatct cgatgtcgag ggtcggatgg agggggctcg ctgtcgtcgt ggcgatcgtc
     2701 gtcgctgcgg gcacgttcgg caccctcgcc gtggaccgcg tgacgacggc ggatcgctgt
     2761 ccgccaacga cggcccacgc tgactggtcc gtcgcacgtc gctgggacga ggcgctcctc
     2821 gacgcgatcc ggcgcgccct gccgaacccg ccggttcacg cccgcaacct gttccacacc
     2881 tcggtcgcga tgtgggacgc ctgggcgacg tacgacccga cggcctccgg cgacctcttc
     2941 accgagaagc acaccacgct ggacgtcgcc gcggcccgga acgaggcgat cagctacgcg
     3001 gcctatcggg tcctgagcgc gcgctacatc aaggccgtcg ggggctccga gtcgctcaac
     3061 gagttcgacg acctcatgga caccctctgc tatccgatcg ccgacaccgg cacgaccggc
     3121 aacgagcctg ccgcggtggg caaccggatc gcggccgccg tcctcgcggc cggcagggcg
     3181 gacggctcga acgaggccgg cggctacgcg gcgcccgact acaagtcggt caacccgccc
     3241 ctggtcgtca accagacggg gacgacgctc gtcgacccga atcgctggca accgctccag
     3301 ctcgagcaca tgatctcgca gaacggcatc ccggtgacca acggcgtcca gcaggcgatc
     3361 gggccgcact ggggtcacgt cacctcgttc gccctgccga cgggcggcgc agccggcacg
     3421 ccgatcgatc ctggaccgcc accccaactc ggcaacccgg cgaccgatca ggcgtacaag
     3481 gaccaggccg tcgaggtcat ccgcgactcg agtgagctcg acgccgcggc aggagtgatg
     3541 atcgatgtct cgcccggggc gcgcggcggc aacaccatcg gcacgaacga cggcagcggc
     3601 catcccgtcg acccggc
//
LOCUS       DATAIW010000154         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_7583, whole genome shotgun sequence.
ACCESSION   DATAIW010000154 DATAIW010000000
VERSION     DATAIW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7583"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VHM48_04935"
     CDS             <1..1855
                     /locus_tag="VHM48_04935"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HEX2754783.1"
                     /translation="SPVTTTLVAGASTTVNKTLALDGLPARADIIVAIDTTASMGTPI
                     AQAQADATNICTTVKASIPGARFAVVALGDYPGMPSGGPGDNPYALLTPGFLSSCPAF
                     SAAIATMTAHDGGDLPEAYNRTFLEAYSDSAYSAPVALGGRDPLASQFLVVLGDATPH
                     SAVGFGSCVNAPPDDFGRDGAPGGGDDITSAAAIAGLVANNITLLMIRYTTGGVSVAL
                     PCYNDLATATGGAAVDDSGAGTIGPFIVSKAKVVPYSADLVVSAGCPIGFSFNPTFPT
                     GMLTGPQTIPFVETITAPTTPGSYTCTITAVTNPGGTTNAVETVNLTVTPAAPATLDL
                     QPSTATNVVDAQHCVTATVKDAFGNVTPGITVDFSVSPTTFRTPSSGTAVTDATGQAQ
                     FCYTSALPGSDTITAFADTDNSGAQNGTEPSATAAKTWVLPGSGAGCKVTYGGRITTA
                     DGDKATFGGNAKGTGPSGQEEYQDHGPALVMNVHSIDVQAVVCSADGTSASIFGTATI
                     DGSGSVDYRIDVTDLGEPGSSDTYQIRLSNGYDSGLQTLSGGNVQIHKALKAASAHTA
                     SAGVKAHTAPSGKGHTAPKAGKPSTAPAAPTVPTADKGNRDHKVSKANKAH"
     gene            2061..2252
                     /locus_tag="VHM48_04940"
     CDS             2061..2252
                     /locus_tag="VHM48_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2754784.1"
                     /translation="MAPDATGGVYVNFLHNDEGEARVRAAHGDRYDRLAQVKARYDPD
                     NVFRSNKNIKPAGAGAGSS"
BASE COUNT          415 a          848 c          733 g          320 t
ORIGIN      
        1 cagcccggtg acgacgaccc tggtcgccgg cgcctccacc accgtcaata agaccctggc
       61 gctcgatggc ctgccagccc gggcggacat catcgttgca atcgacacga ccgcgagcat
      121 gggcactccg atcgcccagg cccaggctga cgccaccaac atctgtacga ccgtgaaagc
      181 ctccatcccg ggcgcgcgct tcgcggtggt ggccctggga gactatcccg ggatgccgag
      241 cggcgggccc ggcgacaacc catacgccct gctgacgcca ggcttcctct cgagctgccc
      301 cgcgttctcg gcggcaatcg ccacgatgac tgcccacgac ggtggcgacc tccccgaggc
      361 gtacaaccgg acgttcctcg aggcctattc ggactcggcg tacagcgccc ctgtcgcctt
      421 gggcgggcgc gacccgctcg cgagccagtt cctcgtcgtc ctcggcgacg caaccccgca
      481 cagcgcggtc ggcttcggct cgtgcgtcaa cgcgccaccc gacgacttcg ggcgcgacgg
      541 cgctcccggt ggcggcgacg acatcacctc cgcggccgcg atcgcgggcc tcgtcgcgaa
      601 caacatcacc cttctgatga tccgctacac gaccggcggc gtgtcggtcg cgcttccctg
      661 ctacaacgac ctcgcgaccg cgaccggcgg cgctgcggtc gacgacagcg gcgccgggac
      721 gatcggcccg ttcatcgtct caaaggcgaa ggtcgtgccg tactcggcgg acctggtcgt
      781 ctcggcgggc tgcccgatcg gcttcagctt caacccgacc ttcccgaccg gcatgttgac
      841 cgggccgcag acgatcccgt ttgtcgagac gatcacggca ccgacgaccc ctggtagcta
      901 cacctgcacg atcaccgcgg tcacgaaccc gggcgggacg accaacgcgg tcgagacggt
      961 caacctgacg gtcacgccgg ctgcgccggc cacgctcgac ctccagccga gcaccgcgac
     1021 caatgtcgtc gacgcgcagc attgcgtgac cgcaacggtc aaggatgcgt tcggcaacgt
     1081 gacgccggga atcaccgtcg acttctcggt gagcccgacg acgttccgca cgccatcgag
     1141 cggcacggcg gtcacggatg cgaccggcca ggcccagttc tgctacacct cggcgctgcc
     1201 tgggagtgac acgatcaccg ccttcgccga caccgacaac agcggcgccc agaatggcac
     1261 cgaaccgagc gccaccgccg cgaagacctg ggtcctgcct gggagcggag ctggctgcaa
     1321 ggtcacgtac ggcggccgga tcacgaccgc ggacggtgac aaggccacct tcggcggcaa
     1381 cgccaaaggc acgggccctt cgggccaaga ggaataccag gaccacggcc ccgccttggt
     1441 catgaatgtc cattcgatcg acgtccaggc cgtcgtgtgc tcggctgacg gcacgtcggc
     1501 gagcatcttc ggaacggcaa cgatcgacgg atcgggctcg gtcgactacc gcatcgacgt
     1561 caccgacctg ggcgagcccg ggtcgtccga cacgtaccag atccgcctga gcaacggcta
     1621 tgactccggc ttgcaaacgc tctccggagg taacgtccag atccacaaag ccctgaaggc
     1681 cgcaagcgcc cacacggcgt ccgcaggcgt caaggcccac accgctccct cgggcaaggg
     1741 gcacacggcc cccaaggccg gcaagccttc cacggctccg gctgctccca cggttcccac
     1801 tgccgacaag ggcaacaggg accacaaggt cagcaaggcc aacaaggccc attagggagt
     1861 cgccggcgta agctcgagag gtcgagcccg ccgggagcga tgagctcggg cgagcaaacc
     1921 acagtcagaa cgaggcgggc ccgtcgggcc cgcctcgtgc ctttccttag tacggagtcg
     1981 ccgaaagtcg ttcggagtcg cctgaagagg gcggtccgga gcatccggat atcggctggt
     2041 gccgggagta cgccaccgcc atggctcccg acgcgaccgg cggcgtctac gtcaatttcc
     2101 tgcacaacga cgaaggcgag gctcgtgtcc gcgcggcgca cggcgaccgc tacgatcgac
     2161 tggcccaggt caaggcccgc tacgacccgg acaacgtctt ccggtcgaac aagaacatca
     2221 agccggcggg tgccggcgcg gggtcgtctt gagatcggcc acggccgggc agtgcaagtt
     2281 cacccacgtc gcgctgatct ttgacaccgg cattcg
//
LOCUS       DATAIW010000155         9159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_37699, whole genome shotgun sequence.
ACCESSION   DATAIW010000155 DATAIW010000000
VERSION     DATAIW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37699"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..487)
                     /locus_tag="VHM48_04945"
     CDS             complement(2..487)
                     /locus_tag="VHM48_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754785.1"
                     /translation="MYTDALSFLEDERDAWRPYETLTMLTDEELERAVEGAHGWSGRD
                     LMGHLLTWQLLSLDVARELAMSEQSISRQQAEADWEERGGDVVNAEAEAAWRTKTIGE
                     VRQEFARVAGELRGYLTVVPETRWLKNAEQQRFFYEETIEHYADHEADLAAILAAAGR
                     S"
     gene            558..2486
                     /locus_tag="VHM48_04950"
     CDS             558..2486
                     /locus_tag="VHM48_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754786.1"
                     /translation="MPDDPTPIPTAQTPPPSPRAEPLTVATPGEPPAGQRRTGVVFVH
                     GIGTQVARETLFDWARPIIDVLGAWRREYDESNPAATIGENPVGSASVSDLANPWIEV
                     DVPAFAGRERAQWLFTEAYWAGDVRPPSFTDSARYLLRRIPGIAFGIAQGYGPREDRR
                     NDRLQDLLDQNKDSPDPVVKARVAELRLSLSKRWRITDALDHVLQYKIVRAILAGVGT
                     TVALTGLAIYAALHAIPIDAVRKKVDIALADTFIVEWFGDLPVLLDDQAQSAAIRTRL
                     LERVSWLKANRCDDVVLVAHSGGTIVSFAALLRYDRAAFDVAKLVTLGEAIKLGWHLE
                     AEAHDWVPGNTIRGDVTVNHPDLRWVDVWASYDPAPSGELVGIDGCPMIPVEKVSDRR
                     DGEGIQIESRPVTNFMHLGLDHGGYWANDEGFLIPLIRHIDDPTGDGTASRFYRNSLD
                     RTIRTERRRRRVAILLAWRWTAFALAIAAVIAAVVRSSLADTGDAVAGVWSLLPAHQL
                     VSGPIDGVGSAIAVILRSIGRVEIDDGLRAAGPVVLGALVPLAATFMIYRRGVGSWMA
                     HDALERIAIRREQFTAAGRRTARSEAVLLVGGLIAIVLAALVVPIGALIAWLVAVAVV
                     AAVARRVGADADHPASLG"
     gene            complement(2523..4289)
                     /locus_tag="VHM48_04955"
     CDS             complement(2523..4289)
                     /locus_tag="VHM48_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2754787.1"
                     /translation="MTWSARKRAWEAQRLADAIGRPPERKTRFSTISDTPIERLYGPW
                     SWQPVADGSPAGGGGPTLLDHHGDHLRGEDGRWDDFDPLRDVGLPGEPPFTRGIHPTG
                     YRSRLWTMRMFAGFGAAEDTNARFRSLLAAGQTGLSIAYDMPTLYGYDTDDPEAEGEF
                     GTCGVAVSSLADMEVLLGGLPLDKVSTSMTINSPAAPIWAMYIVAAEKAGVPRAALEG
                     TTQNDILKEFVAQKEFLFPPEPSMRLVTDTIEFGTNELPRWNTISISGYHIREAGSTA
                     IQELAFTIADGMAYVEHALARGLRVDDFAPRLSFFFNSHSDFFEEIAKFRAARRIWFK
                     LMSERYRAENERSTWMRFHTQTAGVSLTSQQPLNNLTRVATQALAAILGGTQSLHTDA
                     YDEALAVPTAEAALLALRQQQVIAEETGVANTIDPLGGSWFVEALTNETEREVWRYLD
                     EIDRRGGMVAAIGEGYPQHEVADAAYRYQREFDAGERRVVGVNAYVDENEQTPIPVLS
                     VPPGSFEAQMARLERTRRERDPEAVAGALAGLRDAASRPGTSETNLMPHFLRCAAAYA
                     TLGEQCAVLRGVFGEYREPVLV"
     gene            complement(4359..4553)
                     /locus_tag="VHM48_04960"
     CDS             complement(4359..4553)
                     /locus_tag="VHM48_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754788.1"
                     /translation="MLKTIARVLLLRILPRRILPIVTVVEILLFLRSIRNRSRVTVNG
                     PTASRTAPPARASGERQTHA"
     gene            complement(4638..5291)
                     /locus_tag="VHM48_04965"
     CDS             complement(4638..5291)
                     /locus_tag="VHM48_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754789.1"
                     /translation="MSPCPRPRSTTRSRRGRGSIGRPIVVGFALLLGLVLSGCAAPFD
                     PTGPCTADGNAPGAYPELEAAVPTAYQGAKPSELDSGRACTAAGLGTLSGHGVKELRF
                     AGGTWLTGTDSGLSLAIFTNVEGPALDPAWMAEFYEAGARQGKNVTSVETTVYAVTTG
                     ITGSRLDVLNGESYQSVVVWERNGQVAVTVVADFIREIQTKDAHDAIVRAAVDAFVG"
     gene            complement(5786..7624)
                     /locus_tag="VHM48_04970"
     CDS             complement(5786..7624)
                     /locus_tag="VHM48_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEX2754790.1"
                     /translation="MLGTAAIPSAGSGAPADDLDRTLGPDVRAPALLARMTVDEKIGQ
                     MTQLESDSVRPAGVTQFLLGSVLSGGDGTPPANTPAAWYEMVSAYQQAALGTRLGIPI
                     LYGIDAVHGNGKVVGATIFPHNVGLGALDDPALVSEIGRATAREMTAIGVRWDFGPVL
                     AVPQDVRWGRTYEGYGEDPALVERLSVAFITGLQGTDLAVDGSVAATAKHFLGDGGTT
                     WGSASTNGFSIDQGVTGGDEATLRAIHLGPYRTAIGAGARIVMASFSSTSAGKVHGDH
                     HLLTDVLKGELGFTGFVVSDWGGVDQVDPDYATAVARSIMAGVDMVMVPSDAQRFQTA
                     VRQGLASGAINPDRIDDAVLRILRVKFEMGLFEQAMPPAGDAGDVGSSPDRALARTAV
                     AGSAVLLKASSGVLPIGATGTVLLSGPAADDIGRQSGGWTVTWQGATGATTPGTTIAD
                     ALAAGLGARLTYDGRTAFGPDTRGDTGIVVVGEYPYAEGRGDSATLALPPDDLALVAA
                     MRPRVAHLVVVVLSGRPVILDEILDQADALIAAWLPGTEGTGIADVLLGDTPFRGTTP
                     YTWPATPDDAPRTGRGSCAGAVFPVGFGLDATGLRLGPAACAGTTP"
     gene            complement(8129..8893)
                     /locus_tag="VHM48_04975"
     CDS             complement(8129..8893)
                     /locus_tag="VHM48_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2754791.1"
                     /translation="MPKRDPHDILGVDPGASPTQIKAAWRRLARTHHPDLTGDDPAAS
                     RIATQRMAEINDAYAALTRDGGRLGTRWAGPDVGASDAGPAGTAGRGTAKSQRGGPPK
                     PKATRPVTARVDTTWNVRPRNQTLHVPTGGASGPIVRAQPPLRVTSEDREPPRASTPT
                     GPLARSRVRNFRRPAAPPLQEALDLELAFGKFHGHTLGQIAAFEPSYIDWLAATITRD
                     PDLIAAARVVRAELDRKGVARRARAAGDVAGSGRSA"
     gene            8970..>9159
                     /locus_tag="VHM48_04980"
     CDS             8970..>9159
                     /locus_tag="VHM48_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754792.1"
                     /translation="MRPIRRAGIVAAIAAAPLALAWRFAILYRVRAGHPTPRPPQLTP
                     ADLGMPFEEIEVPTTDGLT"
BASE COUNT         1271 a         3185 c         3345 g         1358 t
ORIGIN      
        1 gtcacgaccg gccggcggcg gcgaggatgg ctgcgagatc ggcctcgtga tcggcgtagt
       61 gctcgatcgt ctcctcgtag aagaagcgct gctgctcggc gttcttgagc cagcgtgtct
      121 cgggcacgac cgtcaggtaa ccgcgcagct caccggcgac gcgggcgaac tcctggcgga
      181 cttcgccgat cgtcttcgtc cgccaggcgg cctcggcctc ggcgttgacg acgtcgccgc
      241 cgcgctcctc ccagtcggcc tcggcctgct ggcgggagat cgactgctcg ctcatcgcca
      301 gctcgcgagc cacatcgagc gacagcagtt gccaggtcag gaggtggccc atgaggtcgc
      361 gcccggacca gccgtgcgcc ccctcgacag ctcgctccag ctcttcgtcg gtgagcatcg
      421 tcagcgtctc gtacggccgc catgcgtcgc gctcgtcttc gaggaaggag agggcatcgg
      481 tgtacatcgg cgcatcgtag cgggccggcc gcccggatgg cgacgcaaca gcgacagcca
      541 cgcgttacgg tgccgccatg cccgacgatc cgacgccgat cccgaccgcg caaacgccgc
      601 cgcccagccc ccgagccgag ccgctcacgg tcgcgacacc cggcgagccg ccggccggcc
      661 agcggcggac cggcgtcgtg ttcgttcacg gcatcgggac ccaggtcgcg cgggagacgc
      721 tcttcgactg ggcccggccg atcatcgatg tcctcggcgc ctggcgacgc gaatacgacg
      781 agtcgaaccc ggccgcgacg atcggcgaga acccggtcgg gtcggccagt gtgtcggacc
      841 tggcgaaccc gtggatcgag gtcgacgtgc ccgccttcgc cggccgggaa cgggcccaat
      901 ggctgttcac cgaggcctac tgggcgggcg acgtccgacc gccgtcgttc accgactcgg
      961 cccgctatct cctccgccgg atcccgggca ttgccttcgg gatcgcccag ggttacggcc
     1021 cccgggagga tcgccggaat gaccggctcc aggacctcct cgaccagaac aaggacagcc
     1081 ccgacccggt cgtcaaggcg cgggttgccg agctccgcct ctcgctgagc aagcgctggc
     1141 ggatcaccga cgccctcgac catgtcttgc agtacaagat cgtccgggcg atcctggcgg
     1201 gcgtggggac gacggtcgcg ctgaccggcc tcgcgatcta cgccgccctc catgcgatcc
     1261 cgatcgatgc cgtccgcaag aaggtcgaca tcgccctcgc cgacaccttc atcgtcgaat
     1321 ggttcggcga cctgccggtg ctcctcgacg accaggcgca gtcggcggcg atccggacgc
     1381 ggctgctcga acgtgtcagc tggctgaagg cgaaccgctg cgacgacgtc gtcctggtcg
     1441 cccattccgg cgggacgatc gtcagcttcg ccgccctcct ccgctacgac cgggcagcgt
     1501 tcgacgtggc caagctggtg acgctcggcg aggcgatcaa gctcggctgg cacctcgagg
     1561 ccgaggccca cgactgggtg cccggcaata ccatccgcgg cgacgtcacg gtcaatcatc
     1621 ccgacctccg ctgggtcgat gtctgggcct cctacgatcc ggcgccgtcg ggcgagctgg
     1681 tcgggatcga cggctgcccg atgatcccgg tcgagaaggt ctcggatcgg cgcgacggcg
     1741 aggggatcca gatcgagagc cggccggtca cgaacttcat gcacctgggg ctcgatcacg
     1801 gcggctactg ggccaacgac gaaggcttcc tgatcccgct gatccggcac atcgacgacc
     1861 cgaccggcga cggcaccgcg tcccgcttct accgcaattc cctcgaccgg accatccgga
     1921 ccgaacgccg ccggcggcgg gtcgcgatcc tcctggcgtg gcgctggaca gcctttgccc
     1981 tcgcgatcgc cgcggtgatc gcggccgtgg ttcggtcgag cctggcggac acgggcgacg
     2041 cggtcgccgg ggtgtggtcg ctgctgccgg cgcaccagct cgtcagcggc ccgatcgacg
     2101 gagtcgggag cgcgatcgcg gtcatcctcc gatcgatcgg ccgggtcgaa atcgacgatg
     2161 ggcttcgcgc cgccgggccg gtcgtgctcg gggccctggt gcccctcgcg gcgaccttca
     2221 tgatctaccg ccgcggtgtc gggagctgga tggcccacga tgccctcgag cggatcgcga
     2281 tccggcgcga gcagttcacg gcggcgggcc ggcggaccgc ccgatccgag gcggtcctcc
     2341 tggtcggcgg gttgatcgcg atcgtccttg ccgcgctcgt cgtgccgatc ggggcgctga
     2401 tcgcgtggct ggtggcggtc gcggtggtgg cggcagttgc ccgccgggtc ggcgcggacg
     2461 ccgaccatcc ggcctcgctc ggctgaaccc gcgtcgatcg ggcgcacggc ctggggcggc
     2521 cctcagacga ggacgggctc gcggtactcg ccgaagacgc cgcgcaggac agcgcactgc
     2581 tcaccgagcg tcgcgtaggc cgcggcgcag cggaggaaat ggggcatcag gttggtctcg
     2641 ctcgtgccgg gccgcgacgc ggcatcgcga agacccgcga gggccccggc gaccgcctcc
     2701 ggatcccgct cgcggcgcgt ccgctcgagt cgcgccatct gggcctcgaa cgagcccggc
     2761 ggcaccgaca ggaccgggat cggggtctgc tcgttctcgt cgacgtaggc attgacgccg
     2821 acgacccgcc gctcaccggc gtcgaactcg cgctggtagc ggtacgccgc gtcggcaacc
     2881 tcgtgctggg gatagccctc cccgatcgcg gcgaccatcc cgccgcgccg gtcgatctcg
     2941 tcgagataac gccagacctc gcgctcggtc tcgttggtca gggcctcgac gaaccagctc
     3001 ccgccgagcg ggtcgatcgt gttcgcgacc ccggtctcct cggcgatcac ctgctgctgg
     3061 cggagggcga ggagggccgc ctcggcggtc ggcaccgcga gtgcctcgtc gtaggcgtcg
     3121 gtgtggagcg actgcgtccc gccgaggatc gcggcgaggg cctgggtcgc gacccgggtg
     3181 aggttgttga gcggctgctg ggacgtcagc gagacgccgg cggtctgggt gtggaaccgc
     3241 atccacgtcg aacgctcgtt ctccgcgcgg tagcgctcgc tcatcagctt gaaccagatc
     3301 cggcgggcgg cccggaactt ggcgatctcc tcgaagaagt cgctgtggga gttgaagaag
     3361 aagctgagcc gcggcgcgaa gtcgtcgacc cggaggccgc gggcgagggc gtgctcgaca
     3421 taggccatgc cgtcggcgat cgtgaacgcc agctcctgga tcgcggtcga tcccgcctcg
     3481 cggatgtggt agccgctgat cgagatcgtg ttccagcgcg gcagctcgtt cgtgccgaac
     3541 tcgatggtgt cggtcacgag gcgcatcgat ggttcgggcg ggaacaggaa ctccttctgg
     3601 gcgacgaact ccttgaggat gtcgttctgg gtcgtgccct cgagcgccgc ccgcggcacc
     3661 ccggccttct ccgcggcgac gatgtacatc gcccagatcg gggcagccgg cgagttgatc
     3721 gtcatcgacg tcgagacctt gtcgagcggc aggccgccga ggaggacctc catgtcggcc
     3781 aggctcgaga ccgcgacgcc gcacgtcccg aattcgccct ccgcctcggg atcgtcggtg
     3841 tcatagccgt agagggtcgg catgtcgtag gcgatcgaca ggccggtctg gccggcggcg
     3901 aggagtgagc ggaagcgggc gttcgtgtcc tcggcggcac cgaagccggc aaacatccgc
     3961 atcgtccaga ggcggctccg gtagccggtc gggtggatgc cgcgggtgaa gggcggctcc
     4021 ccgggcaggc cgacgtcgcg gagcgggtcg aagtcgtccc agcggccgtc ctcgccgcgg
     4081 agatggtcgc cgtggtgatc gagcagggtc gggccgccgc caccggccgg gctgccgtcg
     4141 gccaccggct gccacgacca cgggccgtag aggcgctcga tcggagtgtc gctgatcgtg
     4201 ctgaaccgcg tcttgcgttc cggggggcgg ccgatcgcgt cggcaaggcg ttgggcctcc
     4261 caggcgcgtt ttcgggccga ccaggtcatg gcccggacct tagcacgagg tccggtgggg
     4321 ctcggccggg cgacggttcg tggagtcgac gggggccttc aggcgtgcgt ttgccgctcg
     4381 cccgacgccc gcgcgggggg cgcggtccgg gacgccgtcg ggccattgac cgtcacccgc
     4441 gaccggttcc ggatggatcg caggaagagg aggatctcga cgaccgtcac gatcggcagg
     4501 atccgccgcg ggaggatgcg caggagcagg acgcgggcga tcgtcttcag catcgcccga
     4561 tcatcggtcc gcgcgaccac cgtgcggggt tcgcactcga ccgccggcat gtcgcctgcg
     4621 ttgcgacttg catcggccta gccgacgaac gcgtcgacgg cggctcggac gatcgcgtca
     4681 tgggcgtcct tggtctggat ctcgcgaatg aagtcagcga ccaccgtgac cgcgacctgg
     4741 ccgttccgtt cccagacgac gaccgactgg tatgactcgc cgttgaggac gtcgagccgg
     4801 ctgccggtga tgccggtggt gaccgcgtag accgtggtct caaccgaggt cacgttcttg
     4861 ccctgtcggg cgcccgcctc gtagaactcg gccatccagg cgggatcgag ggccggcccc
     4921 tcgacgttgg tgaagatcgc caggctcagg ccgctgtcag tgccggtcaa ccaggtgcca
     4981 ccggcgaacc gcagctcctt gacgccgtgc ccgctcaggg tgccgagacc ggcggccgtg
     5041 caggctcgac ccgagtcgag ctcgctcggc ttggcaccct ggtaggcggt cggcaccgcc
     5101 gcctcgagct ccggataggc gccgggcgcg ttgccgtcgg cggtgcacgg accggtcgga
     5161 tcgaagggcg cggcgcagcc gctcagcacg agccccaaca ggagggcgaa cccgacgacg
     5221 atggggcgtc cgatcgaacc gcgaccgcgt cgcgagcgcg tggtggatcg aggtcgcggg
     5281 cacggggaca tggcgcgatg atacggcgcg atgccgctcg agccccccgg ttccgtctcc
     5341 gcgccgacgc ctggcaagat cgcatgtcac ggtacggcgc ggtcgtttca ggttcgttgc
     5401 agatcggccg gtgcgtcgcg acacctggta cggccgtccc ggtgtggtgc gtggatgcgc
     5461 cccaccatcg ccgccggcgt ttcgggacct gtcggtcatt ccgtcatcga cgattcaccg
     5521 agtacggttc ggttgcgccg taacgcgaac atcccgggaa ccacctgggg cgtcggtcac
     5581 ggatcagggg tcggccttcg gttcactcga tgacggttcg agcgccgagg atcccggacc
     5641 cagatcacga gccgagtaac cacggatcat tcggccagca ggcctgggcc gcggtacggc
     5701 agagcttatc gctctcttca ttcgcgctcc tccgatcgct ctccgggttt ccggagggcg
     5761 atcgccacgt ttccggcact cgttgtcacg gagtcgtccc cgcgcaggca gccgggccga
     5821 gccgcaggcc ggtggcatcg agcccgaacc cgaccgggaa gaccgcgcca gcacaggatc
     5881 cgcgaccggt cctcggggca tcgtccggcg tcgcgggcca ggtgtacggg gtcgtgcccc
     5941 ggaacggcgt gtcccccagc aggacgtccg cgatcccggt gccctcggtc cccggcaacc
     6001 aggcggcgat cagggcgtcg gcctgatcga ggatctcatc gaggatgacc gggcgtccgg
     6061 agagcacgac gacgacgagg tgagccaccc gcggccgcat ggcggcgacg agggcaaggt
     6121 cgtcgggcgg cagcgcgagc gtcgccgaat cgccgcggcc ctcggcgtac gggtattcgc
     6181 cgacgaccac gatgccggtg tcgccgcgcg tgtccggacc gaatgccgtc cggccgtcgt
     6241 aggtgaggcg cgcgccgagg ccggcagcga gcgcgtcggc gatggtcgtg cccggcgtgg
     6301 tcgcgcccgt tgcgccctgc caggtgacgg tccagccgcc tgactggcga ccgatgtcgt
     6361 cggccgccgg cccgctcagg aggaccgtcc cggtggcgcc gatcggcagg acgccggagc
     6421 tggccttgag gaggaccgcc gagcccgcca cggccgtccg ggcgagcgct cgatcgggag
     6481 acgagccgac atcccccgcg tccccggcgg gcggcatcgc ctgctcgaag aggcccatct
     6541 cgaacttgac ccgaaggatc cgcagcaccg cgtcgtcgat ccgatccggg ttgatcgcac
     6601 ccgaggccag tccttgcctg acggctgtct ggaagcgttg cgcgtcggac ggaaccatga
     6661 ccatgtcgac tccggccatg atcgaccggg ccaccgcggt ggcgtagtcc gggtcgacct
     6721 gatcgacgcc gccccagtcc gagacgacga accccgtgaa gccgagctcg cccttcagga
     6781 cgtcggtcag caggtggtga tcgccgtgga ccttgccggc cgacgtgctc gagaacgagg
     6841 ccatgacgat gcgcgcaccg gccccgatgg ccgtcctgta cgggccgaga tggatcgcgc
     6901 ggagggtggc ctcgtcgccc ccggtcacgc cctggtcgat cgaaaagccg ttcgtcgatg
     6961 ccgagcccca ggtcgtgccg ccgtcgccga ggaaatgctt ggcggtagcg gccacgctgc
     7021 cgtcgacggc gaggtcggtg ccctgcaggc cggtgataaa ggcgaccgac agccgctcga
     7081 cgagcgccgg gtcctcgccg tagccctcgt acgttcgtcc ccagcggacg tcctgcggca
     7141 cggcgaggac cggaccgaag tcccagcgaa caccgatcgc ggtcatctcc ctggccgtcg
     7201 cccgcccgat ctcgctgacg agggcgggat cgtcgagagc gccgaggccg acgttgtgcg
     7261 ggaagatcgt ggccccaaca accttgccgt tgccgtggac ggcgtcgatt ccgtagagga
     7321 tcgggatccc gagcctcgtc ccgagggccg cctgctggta cgcactcacc atctcgtacc
     7381 aggcggcggg ggtgttcgcg ggcggcgttc cgtcgccgcc gctcagcacc gatccgagca
     7441 ggaattgggt cacgcccgcc gggcgaaccg agtcgctctc gagctgggtc atctggccga
     7501 tcttctcgtc gaccgtcatt cgagccagaa gggccggagc gcggacgtcc ggtccgaggg
     7561 tccggtcgag atcgtcggcc ggagcccccg agcccgcgga cgggatcgcg gccgtcccga
     7621 gcaccgatga accagagctc gggtggggcg atccacaggc tgccaccgcg aggacaacgg
     7681 ccgaaagccg cgcgaggcag gcgcgccgac agctccgccg gggctgggtg agagtcaccg
     7741 tcgggccgac acgtcgagca ctggtggagt attgccccga tcagcgccgg aggcgagggc
     7801 cgggcggccc tccgcgacgc gccggacgct cgtcgtggtt cgattgggga gcgaggccgg
     7861 cggcggcgtg cgaatagccg aatacgccgg cgggcgcagc cggacgctcg tcgtggttcg
     7921 accgcggagc gaggccggtg gcggcgtgcg aatagccgaa tacgccggcg cgatggcgca
     7981 cgccgcccgg gcagacggac ccattggcgc tcggcgcgac gtggagccgg gggtgtcacc
     8041 gctggggcac gccgctccgg tcgcggttca ttcggctatt tgaacgggca gcgccgggcg
     8101 acccggggtc ggcgccgaat cgagccgatc aggcggatcg accacttccg gcaacatcgc
     8161 cagcagcacg ggcccggcgc gcgacaccct ttcgatcgag ctcggcgcgg acgacccggg
     8221 ccgcggcgat gaggtcgggg tcgcgcgtga tcgtcgccgc cagccagtcg atgtaggacg
     8281 gttcgaaggc cgcgatctgg ccgagcgtgt gaccgtggaa cttgccgaag gcgagctcga
     8341 gatcgagggc ctcctggaga ggtggcgcgg ccggccgacg gaagttgcgc acgcgcgatc
     8401 gagcgagcgg gcccgtcggc gtgctcgccc gcggcggctc acggtcctcg ctcgtgaccc
     8461 gcagcggcgg ctgggcgcga acaatcgggc cggacgctcc gccggtcggc acgtgcaggg
     8521 tctggttgcg cggtcggacg ttccaggtcg tatcgacccg ggccgtcacc ggccgggtgg
     8581 ccttcggctt cggcgggccg ccgcgctggg acttggcggt gccgcggccg gcggtcccag
     8641 ctggaccggc gtcgctcgcg ccgacatcgg ggcctgccca gcgtgtcccg agtcgcccgc
     8701 catcgcgggt cagggccgcg taggcgtcgt tgatctcggc catccgctgg gtcgcgatcc
     8761 gcgaagcggc cggatcgtcc ccggtcaggt cgggatggtg cgtccgggcg agccgccgcc
     8821 aggcggcctt gatctgcgtc gggctcgcgc cggggtcgac gccgaggatg tcgtggggat
     8881 cgcgttttgg cacggttcga gtctatcggt cggacgccga tggggcagcc catttgacag
     8941 gtcggcgggc ggggccgtcc cattgtcggg tgagaccgat ccgtcgagcg ggcatcgtcg
     9001 ccgcaatcgc cgccgccccg ctggctctcg cctggcgttt cgcgatcctc tatcgcgtcc
     9061 gagcgggcca cccgacgcca cggccgccgc agttgacgcc ggccgacctc gggatgccct
     9121 tcgaggagat cgaggttccg acgacggacg gcctgaccc
//
LOCUS       DATAIW010000156         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_67765, whole genome shotgun sequence.
ACCESSION   DATAIW010000156 DATAIW010000000
VERSION     DATAIW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67765"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VHM48_04985"
     CDS             <1..1484
                     /locus_tag="VHM48_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX2754793.1"
                     /translation="WTLPPIEMLDPQPAARVGITIDHERNTKRIEEKLLSFAIPARVV
                     AINSGPVVTQYEVKPEHHVKVSRIEAVADDLAMALSARSIRIEAPIPGKDVVGIEIPN
                     SVSEVVAFRPLVEESKMLDAPSPLMFALGRDVSGKTYAVDLAKMPHLLVAGATGSGKS
                     VCVNALITSILMHARPDDVRMILVDLKRVELAPYNGLPHLLQHVIVEANEAKAVLNWA
                     VREMEERYKILAGHSVRNIAAYNSRPEVAGEEDDERMPYIVLIIDELADLIMREGRKV
                     EDPIVKIAQKARAVGIHLVLATQRPSVNVVTGLIKANVPSRIAFAMASMIDSRTVLDA
                     PGAEDLIGRGDMLYQPVDLPRPVRLQGVFVGDREVGEITKHWLDQTGGRTFYDEDILA
                     FTDAEEGNGSDGGRFGWLRRYGVDEMTIPAAELVVASNRASTSMLQTKLKLGFSRASR
                     VMDELERYGIIGPQDPRNPASPRQIYGPDNWLRTIDDGDGSDE"
     gene            1594..3084
                     /locus_tag="VHM48_04990"
     CDS             1594..3084
                     /locus_tag="VHM48_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754794.1"
                     /translation="MTTTDDARRATGEPRAEGLRLVPPRPDPSHPDGDRNPDRDPDRD
                     PDRSAGRDPERSAAVNRPAPLPERLFAARELKGVDLYRAERDTKIRARYLAALERGEY
                     RELPGDVYTKGFLRNYALYLGLDPEEVVGQWRRERGDSPPSKTILTVPRPIAQPRPGL
                     QFSPGIVVAALLTILIVGVGVWLGVQVMRFAKPPTLAMTAPREATIELDQTATSFTFE
                     GTSIPGATITIEMAGGSRQASADSTGVWSLTVDLRRGRNEFKIDATDPETGKHALEPA
                     MVVITVPVSEIQPLKLNVDQPVENATFENGAIPVQGTTVNATSVKISATWDGPVGGAP
                     VRSPGPSGAPGGTPAPAPISVAVGADGAWDTDANPLQLTTGRWTITITASNAATTATQ
                     TQARHVSVAYKGVNLVVKIKGGAAWIKVWVDGKIDPTVGRAGRTYRDGQVLTFTGQTS
                     VEVRTGSSGVTLFTLNGQSLGALGKRGIPETWLFKPPAAPALTQRT"
     gene            3087..3626
                     /locus_tag="VHM48_04995"
     CDS             3087..3626
                     /locus_tag="VHM48_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2754795.1"
                     /translation="MGGDPLTELAARLQAACLAAGSTVALAESCTGGMVATAITEIAG
                     SSAYFVGGVVSYSNDAKRDLLGVDPAVLAAHGAVSAQVARDMALGARERFGATRAASV
                     TGIAGPDGGSAAKPVGLTYIGVADDEGVDVRRIVWSGDRQANRRDSAIAVLEMLLGRV
                     EGHDGSPLATTATAGRDTE"
     gene            3623..5209
                     /locus_tag="VHM48_05000"
     CDS             3623..5209
                     /locus_tag="VHM48_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2754796.1"
                     /translation="MIGTATPGAGLLPARPARAIAPGERIHVVGAAGAGASAAVLHAA
                     WAGGKPDGCDPGGPSPYTAAIEAAGIALTWRHDASHVTRTPPPDRLAVTKALTAIDPD
                     HPELVAARRAGIPIEPWQQVIADAASGRTLIGVAGTHGKSTSAGWLVHVLHTAGADPS
                     AFVGALLPAALAGGVAATARRGAGATFVVEADEYAGNFDAYRPAIAIVTSAEWDHPDV
                     FADRAAVVASFDRWLRAATSAVTDAPGAAGDPQIAVINVADAGAAELAAGLADWPGTI
                     LATAVVDADAARIASFGRGLAEQYATAAGPARTLLGRIVAQEPDTTTIEIHGFDDLRG
                     SRSARLPTAGRHNAANALGVAGAALVAGIAPDALVSGLASFGGVGRRLERKGEVAGVL
                     VFDDYGHHPTAIRATLGAVRQRAPGRRVWAVYEPLTYHRTAAMLDDFAAVLAEADAVA
                     IAEIWAGRDPDTTIATATGLAEAVARLNPAIPVAAPGSVEATAAWLSREVRSGDAVLV
                     MGGGRSYRIGELLLEKLADR"
     gene            5298..5792
                     /locus_tag="VHM48_05005"
     CDS             5298..5792
                     /locus_tag="VHM48_05005"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX2754797.1"
                     /translation="MAGDVSFDVVSDFDEQELRNALDQVRREVGQRFDFKGVTVDLEQ
                     SKDELTLVTDDEHRAAAIKDLIESKAVRRSLSLKIFDWGKVEPAGGNKVRQVIALRRG
                     LNDELAKKISKLIREEFPKVKPQIQGDAVRVSGKSRDERQKVIGRLRELEDLPVPLQF
                     ENYR"
     gene            complement(5817..>6162)
                     /locus_tag="VHM48_05010"
     CDS             complement(5817..>6162)
                     /locus_tag="VHM48_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754798.1"
                     /translation="CLVGRSLVGRSRSLVGRSLVGRSRSLVGRSLVGVCRDGRKVPGR
                     GRGHLRCLGPDRYGSDLRPVGDPGLAFCHVAARLYLRSLVRHQRTIGLAAMATTMPTA
                     TTMATMIIRIRA"
BASE COUNT          944 a         2190 c         2208 g          820 t
ORIGIN      
        1 actggacgct gccgccgatc gagatgctcg atccgcagcc cgccgcccgg gtcgggatca
       61 cgatcgatca cgagaggaac acgaagcgga tcgaggagaa gctcctgagc ttcgcgatcc
      121 cggcccgggt cgtcgcgatc aactccgggc cggtggtgac ccagtacgag gtcaagcccg
      181 agcatcacgt caaggtgtcg cggatcgagg ccgtcgccga cgacctcgcg atggccctgt
      241 cggcccgctc gatccggatc gaggcgccga tccccggcaa ggacgtcgtc gggatcgaga
      301 tcccgaactc ggtcagcgag gtcgtcgcct tccggcccct cgtcgaggag agcaagatgc
      361 tcgacgcgcc gagtccgttg atgttcgccc tcggccgcga cgtgtccggc aagacctacg
      421 cggtcgacct cgcgaagatg ccccacctgc tggtcgcggg ggcgacgggc tcgggcaaga
      481 gcgtctgtgt caacgccctg atcacgagca tcctgatgca cgcccggccc gacgacgtcc
      541 ggatgatcct cgtcgatctc aagcgggtcg agctcgcccc gtacaacggg ctgccccatc
      601 tcctccagca cgtgatcgtc gaggcgaacg aggccaaggc cgtcctcaac tgggcggtcc
      661 gcgagatgga ggagcgctac aagatcctcg ccggccacag tgtccggaac atcgccgcct
      721 acaacagccg gcccgaggtc gccggcgagg aggacgacga gcggatgccg tacatcgtcc
      781 tgatcatcga cgagctggcc gacctgatca tgcgcgaggg ccgcaaggtc gaggacccga
      841 tcgtcaagat cgcccagaag gcacgggccg tcgggatcca cctggtcctc gccacccagc
      901 ggccgtcggt caacgtcgtg accgggctga tcaaggccaa cgtcccgtcc cgcattgcgt
      961 tcgcgatggc ctcgatgatc gattcgcgga cggtcctcga cgcgcccggc gccgaggacc
     1021 tgatcggccg cggcgacatg ctctaccagc cggtcgacct gccccgcccg gtccgccttc
     1081 agggcgtctt cgtcggcgac cgcgaggtcg gcgagatcac gaagcactgg ctcgaccaga
     1141 ccggcggccg gacgttctac gacgaggaca tcctcgcctt caccgacgcc gaggagggca
     1201 acggcagcga cggcggccgg ttcggctggc tccgcaggta cggcgtcgac gagatgacga
     1261 tcccggcggc cgagctggtc gtcgcctcca accgggcgag cacgtccatg ctccagacca
     1321 agctcaagct cggcttctcg cgcgccagcc gggtgatgga cgagctcgag cgctacggga
     1381 tcatcgggcc ccaggatccg cgcaaccctg cctcgccacg ccagatctac ggcccggaca
     1441 actggctccg gaccatcgac gacggcgacg ggtccgacga atgaacgatc gtcccatgaa
     1501 cgcaatctgg accggacgcg acgacggggt atcctctgcg tcgaggtcgg ccgccggcgc
     1561 gcgcgcggtt gcggacgacg aggacgggac gcgatgacga cgacggacga cgcgcgacgg
     1621 gcgacggggg agccgcgggc cgagggcctg cgtctcgttc cgcctcggcc ggatccgagc
     1681 caccccgacg gggatcggaa tccggatcgc gacccggatc gcgacccgga tcgctcggcc
     1741 ggtcgcgacc cggagcgatc tgccgccgtg aatcgacccg ccccgctccc cgagcggctg
     1801 ttcgccgcgc gtgagctcaa gggcgtcgac ctctaccgcg ccgaacgcga cacgaagatc
     1861 cgggcccggt acctcgccgc actcgagcgt ggcgaatacc gcgagctgcc cggcgacgtt
     1921 tacacgaagg gcttcctccg caactacgcc ctgtatctcg ggctcgaccc ggaggaggtc
     1981 gtcgggcagt ggcggcgcga gcgcggcgac agccccccga gcaagacgat cctgaccgtt
     2041 ccgcgaccga tcgcccaacc gcggccgggt ctccagttct cgcccggcat cgtcgtcgcg
     2101 gcgctcctga cgatcctgat cgtcggtgtc ggcgtctggc tcggcgtcca ggtcatgcgc
     2161 ttcgccaagc caccgacgct ggcgatgacg gcaccccgcg aggcgacgat cgagctcgac
     2221 cagaccgcga cctcgttcac cttcgagggg acgtcgatcc cgggggccac gatcacgatc
     2281 gagatggccg gcggatcgcg ccaggcctcg gccgattcga ccggcgtgtg gagcctgacc
     2341 gtcgacctcc gccgcggccg caacgagttc aagatcgacg cgaccgaccc ggaaaccggg
     2401 aagcatgccc tggagccggc gatggtcgtg atcaccgtgc cggtcagcga gatccagcca
     2461 ttgaagctga acgtcgacca gccggtcgag aacgcgacct tcgagaacgg tgccatcccc
     2521 gtccagggca ccacggtcaa tgcgacgtcg gtgaagatct ccgcgacctg ggatgggccg
     2581 gtcggcgggg cgccggtacg gagcccgggg ccgtcgggcg caccgggcgg cacgcccgcc
     2641 ccggcgccga tctcggtcgc tgtcggcgcg gacggagcgt gggataccga tgcaaacccg
     2701 ctgcagctca cgaccggccg atggaccatc acgatcaccg cgtccaatgc ggcgacgacc
     2761 gcaacgcaga cccaggcccg ccatgtgtcc gtcgcgtaca agggcgtcaa tctggtcgtc
     2821 aagatcaagg gcggcgccgc ctggatcaag gtctgggtcg acggcaagat cgatccgacc
     2881 gtcggtcgtg ccggccggac ctaccgcgac gggcaggtcc tgacgttcac gggccagacc
     2941 tcggtcgagg tccggacggg atcgtccggg gtgacgttgt tcacgctcaa cggccagtcg
     3001 ctcggggcgc tcggcaagcg tgggatcccc gagacgtggt tgttcaagcc accggccgcg
     3061 ccggcgctca cccagcgaac ctgacgatgg gcggcgatcc gctcaccgag ctcgccgcgc
     3121 gtctccaggc ggcctgcctc gcggcgggat cgacggtcgc gctggccgaa tcgtgcaccg
     3181 gcggcatggt cgccaccgca atcacggaga tcgccggatc gagcgcctac ttcgtcggcg
     3241 gagtcgtgtc gtactcgaac gacgcgaagc gcgacctgct cggcgtcgat ccggcggtcc
     3301 ttgccgccca cggcgcggtc agcgcccagg tcgcccggga catggcgctc ggagcacgcg
     3361 agcggttcgg ggcgactcgc gcggcttccg tcaccgggat cgcggggccg gacggcggat
     3421 ccgcggcgaa gcccgtgggc ctgacgtaca tcggcgttgc cgacgacgag ggcgtcgacg
     3481 tccggcggat cgtgtggtcc ggcgatcgtc aggccaaccg gcgcgacagc gcgatcgccg
     3541 tcctcgagat gcttctgggt cgggtcgagg gccacgacgg gagcccgctc gcgacgacgg
     3601 ccacggccgg gcgcgacacg gagtgatagg gaccgccacc ccgggcgcgg gtctcctgcc
     3661 cgctcgcccg gcgcgcgcga tcgcgcccgg cgagcggatc cacgtggtcg gtgcagccgg
     3721 cgccggcgcc agtgcggcgg tgctccacgc tgcgtgggcg ggcggcaagc ccgacggatg
     3781 cgacccgggc ggtccgtcac cgtacacggc cgccatcgag gcggccggga tcgccctcac
     3841 ctggcggcac gacgcttcgc acgtgacgcg gacgccgcca ccggatcgcc tcgcggtgac
     3901 caaggcgctg acggcgatcg atcccgatca cccggagctc gtcgccgctc gacgcgccgg
     3961 gatcccgatc gaaccgtggc agcaggtgat cgccgacgcc gcttccggcc ggacgctgat
     4021 cggcgtcgcc ggcacgcacg gcaagagcac ctccgcgggt tggctcgtcc atgtcctgca
     4081 caccgcgggc gccgacccgt ccgcgttcgt cggggcgctc ctgccggcgg cgctggccgg
     4141 tggcgtggcc gcgaccgcgc ggcggggggc cggcgccacc ttcgtcgtcg aggccgatga
     4201 atatgccggc aacttcgacg cataccggcc cgccatcgca atcgtgactt cggctgaatg
     4261 ggaccatccc gacgtgttcg ccgatcgggc cgccgtcgtg gcctcgttcg accgctggct
     4321 gcgagcggcg acgtcggccg tgaccgatgc gcccggcgcc gcgggcgacc cccagatcgc
     4381 ggtgatcaac gtggcggatg ccggggctgc ggagcttgcg gccgggctcg cggactggcc
     4441 ggggacgatc ctcgccaccg cggtcgtcga cgccgacgct gcccggatcg cgagcttcgg
     4501 ccgcgggctc gccgagcagt acgcaacggc cgccggtccg gcacggacgc tgttggggcg
     4561 gatcgtcgcc caggagccgg acacgacgac gatcgagatc cacgggttcg acgacctccg
     4621 cggctcgcgc tccgcacgtc tgccgaccgc cggtcgccac aacgccgcga atgccctcgg
     4681 ggtggccggc gcggcgctgg tcgcgggcat cgcaccggac gctctcgtgt ccggtctcgc
     4741 ctccttcggc ggggtcgggc gacggctcga gcgcaagggc gaggtcgccg gcgtcctcgt
     4801 cttcgacgac tacggccacc atccgaccgc gatccgcgcg acgctcggcg ccgtccgcca
     4861 gcgcgcgccc ggtcgccgcg tgtgggccgt gtacgagccg ctgacgtatc accggacggc
     4921 cgccatgctc gacgacttcg cggccgttct cgccgaggcg gacgcggtcg cgatcgccga
     4981 gatctgggcc ggccgcgacc cggacacgac gatcgccacg gccaccgggc tggcggaggc
     5041 cgtcgcgcgg ctgaatccgg cgatcccggt ggcggctccg ggatcggtcg aggccacggc
     5101 ggcatggctg tcgcgggagg tccgcagcgg ggacgccgtc ctggtcatgg gcggtggtcg
     5161 cagctaccgg atcggggagc tcctgctgga gaagctggcc gaccgctgat cggccgatca
     5221 gggcatactt ggcgggtcgt cccagatcgc ggccgtccgt ccgacgcgcc atcgatcgcc
     5281 acaccgcagg gaggcccatg gccggagacg tttccttcga cgtcgtcagc gacttcgacg
     5341 agcaggagct gcgcaacgcg ctcgaccagg tccgccgcga ggtcggccag cgcttcgact
     5401 tcaagggcgt gacagtcgac ctcgagcagt ccaaggacga gttgacgctc gtgaccgacg
     5461 acgagcatcg ggcggccgcg atcaaggacc tgatcgaatc gaaggcggtc cgccgcagcc
     5521 tgtcgctcaa gatcttcgac tggggcaagg tcgagccggc aggcgggaac aaggttcgcc
     5581 aggtgatcgc gctccggcgt gggctgaacg acgagctggc caagaagatc agcaagctga
     5641 tccgcgagga gttcccgaag gtgaagccgc agatccaggg cgacgccgtc cgggtttcgg
     5701 gcaagagccg cgacgagcgc cagaaggtga tcggccggct gcgtgagctc gaggacctcc
     5761 cggtcccgct ccagttcgag aactaccggt agccggccga tgatgcccgg cacgcgctac
     5821 gctcggatcc tgatgatcat cgtcgccatc gtggtggcgg tgggcatggt cgtcgccatg
     5881 gccgccagcc cgatcgttcg ctgatggcgg acgagcgaac ggaggtagag ccgggcggcg
     5941 acgtggcaga aggcgaggcc gggatcgccg acgggccgga ggtcgctgcc gtagcggtcg
     6001 gggccgaggc aacggaggtg cccgcgccct cgcccgggga ccttccggcc atcgcggcag
     6061 acgccgacga ggctccggcc gacgaggctc cgggagcggc cgacgaggct ccggccgacg
     6121 aggctccggg agcggccgac gaggctccgg ccgacgaggc ac
//
LOCUS       DATAIW010000157        12505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_211981, whole genome shotgun sequence.
ACCESSION   DATAIW010000157 DATAIW010000000
VERSION     DATAIW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211981"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3207
                     /locus_tag="VHM48_05015"
     CDS             <1..3207
                     /locus_tag="VHM48_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2754799.1"
                     /translation="LAASLSVALENVRLFDETKRLLSETNERAAELALINDVQHGLAQ
                     KLDMQAMYDLVGDRIQAIFDAQVVDIGVIDREQELVRFPYWIERGVRFPDEPMPLVGP
                     RRQIMETRRPIVVNRDMRARMKELGQDLTLPGEVPQSALWAPLVVGDETRGVISLQNL
                     DREDAFSDSDVELLTTLAASLSVALENVRLIDETRHRLAELATVNEVGKALASQLDLD
                     NLIQLVGDQMRRTFAADITYVALHDVESQRIDFPYYNEFGSATGQDAIPFGKGLTSKI
                     IDTCETLLLNREADWDNLGTRGVGTLSKSFLGVPIVLGDRAIGAISVQSTTQEGRFGE
                     SDARLLSTIAANVGVAIQNARLYREAHRRGDEMAALADVGQEISATLDADVVLRQIGE
                     RVQSLLDADASALFLADPDGRSFRPILALGTIAEQILADTIVEGQGIIGDVIRTRTPE
                     YINDVGADARTVTIPGTEDIAEERLMVAPLVARDRVSGVMAVWRNHGAPFSQADLDFL
                     VGLARQASIAIENSRLFAEAGVARAAAEDANQAKSAFLAAMSHEIRTPMNAVIGMSGL
                     LLETELDDEQRDFADTIRSSGDALLTIINDILDFSKIEAGRVDLVAEPFSPRVAVESA
                     LDVIAPTAARKKVELVYAMGDGLPDAIVGDAGRLRQIVLNLLSNAVKFTDQGEVVLGL
                     EATRGTAAGDPWTLAIEVRDTGIGIPPDAMDRLFQSFSQVDASISRRFGGTGLGLAIS
                     RRLAESMGGALTATSSGVAGEGSTFRLTLPVEVTVLPDAVAPPPIRSLRGCRVLVVDD
                     NATNRKILTTLLQRWEVDAAATASPLEAIGWVRAGQTFDVAVLDLLMPERDGIELAGD
                     LRDLRPDMPIPVVILSSIGQHSRTAPNIRAMLVKPVKPSALHDALATAVSGEEVVARP
                     DRAASASPAAAAARNGNLRILLAEDNAVNQKLALRLLERMGNGGADVVEDGEAVIEAL
                     EKTDYDLILMDVQMPRMDGLEATRRVRARWPQRAVRIVGLTANAMAGDREACLEAGMD
                     DYVSKPIRPDELARAIGATSRGPVASGSSSDEPRGTSS"
     gene            3204..4394
                     /locus_tag="VHM48_05020"
     CDS             3204..4394
                     /locus_tag="VHM48_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2754800.1"
                     /translation="MSELEPRSAGEAPGVMDPSGPAEALDHDAMTGRVLVVDDTPFNR
                     QLLTRLLGRIGHTSLEAADGQAALDLLRAPATEPVDVILLDIMMPVMDGYETLAALKA
                     DKTLGDIPVIVISGVDELDSVVRCIEMGAADYLPKTVDPAILRARIAASLGQKRLRDQ
                     ERALLGTIDRQRAQLARFLSPQVAALVSSPDGEAMLAGHRREITAMFCDLRNFTVFSE
                     TAEPEEVLGFLRLYHATLGALVVAHEGTLEHFAGDGIMVFFNDPVLQTDHAQRSVELA
                     LDIRERFKDISAEWRRRGHVLEVGIGIATGYATMGRIGFEGRYDYGGVGNAIILASRL
                     SGEATAGQILLAARTYAAIDEALAEPVGDRQLKGFSRPMPVYAAGPSDRTASTGADAA
                     EVFG"
     gene            4391..4753
                     /locus_tag="VHM48_05025"
     CDS             4391..4753
                     /locus_tag="VHM48_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX2754801.1"
                     /translation="MTGPIDDAVFASLVEMTGGEMDFVDELVDTYLEDGANQIATMRA
                     AVESGDAEGLTRAAHSLKSGSLNVGALELGGLCRALEEAGRSGAIADPDARIAAIDDA
                     FAAAGTTLLDERGRRTPG"
     gene            4922..5689
                     /locus_tag="VHM48_05030"
     CDS             4922..5689
                     /locus_tag="VHM48_05030"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2754802.1"
                     /translation="MRLEDKVTIITGAGGGMGRTAARMFAAEGARVVCVDVTEEMAES
                     AAADVTAAGGQATAIAADVSQDGQAKRMVDHAIATYGRVDALYNNAGIMPAADHSVID
                     TDVETWDKVMAVNVRGVYLGCKYAIPKMIEQGSGSIINVASFVAILGCSVPQDAYTAS
                     KGAVLALTRSLAVQFAPQGVRSNSISPGPIETPLLMDWLVKDEEAKKIRLARNPTGRF
                     GRPEEIVHVALYLASDESRWTNGANLVIDGGISVNYF"
     gene            5701..6504
                     /locus_tag="VHM48_05035"
     CDS             5701..6504
                     /locus_tag="VHM48_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2754803.1"
                     /translation="MDQAQGTRVAVGVDVGGSGIKVAVVDVDRGVLVGPRLRVATPQP
                     STPDVVVPVIVRTVRRALREAKVDRADVTVGVGMPAVVFDGVTKSAANIDPLWVDFDL
                     GSHLRQALKRSVFVLNDADAAGLAEMRFGAGVGERGVVFVLTLGTGLGSGMFNDGVLV
                     PNTELGHMEIRGRDAERRSAAVARTRRGLSWKAWASDLDEHLHAIDKLFSPNLIILGG
                     GVSKNADKFIPRLTVRPRVVPATLRNDAGIVGAAMAASEKFIEGAASTR"
     gene            6705..9035
                     /locus_tag="VHM48_05040"
     CDS             6705..9035
                     /locus_tag="VHM48_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2754804.1"
                     /translation="MSETKSRPAWAWRVFAVAAPVFALLYLALPGSGPARTLAYPVFG
                     IVASIAIVVGIRRGSPTRPGSWWLIAGALVLMATGDIAYSAIELVGEVPYPSLADAFY
                     LAGYLALVGGVTGLARGRLSGGDRTPLIDGAILAAGAASILWIVVMRPSIVGQVDVLA
                     ATVSLAYPCMDLVILALAFRVVLSGDRPAYLNLLLAGLAIYFAADIIYAMAVLDGTYV
                     DGHPVDALWIIGVTLWGAAALHPSIAHPVRLVVEEDQYLSRSRLALLALASLIAPATL
                     TVQAAMAGEGDAIGLIIAWTILFGLVLIRLASTVDELGGSLLQRRRLEDDLTHQAQHD
                     PLTGLANRSLFERRLTTAIDRRPDTTALVFLDLDDFKTINDTLGHGVGDELLRILADR
                     VRGELRTGDLAARLGGDEFAVLVEHCPDEPTVRAIAERILAAMRKPLVLGGRQLQVRG
                     SAGVAVGRVETTPMDLMRNADVAMYQAKLHGKDQVETYEPRMHSQVVRGYQLRTELAE
                     AIRRQQFVLHYQPVLRLATDEIVGAEALVRWRHPERGLVGPDDFIPQAESSGLIHDLG
                     RWILREACATAAAWPLRPDGTAASVSVNLAPSQLLDPKLVDDVASVLAATGLPASRLY
                     LEITESALVDLDRACTALEGLRGLGIGLALDDFGTGYSALSHLASLPFGIVKIDRGFI
                     RSAREGTRVGNLLAGIVGLCHALDLLTVAEGIETEDDLRLVRRVGFDVGQGYLFAKPI
                     PGPEFAAIYASGRLDRPRPEGQRPTTIGRVVEPTAA"
     gene            complement(9039..10145)
                     /locus_tag="VHM48_05045"
     CDS             complement(9039..10145)
                     /locus_tag="VHM48_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX2754805.1"
                     /translation="MTAPSFRRDGSLASDPVRPAPAVRGRRGRSGRRWVRNVAEVVQG
                     RHIRIWLAIIAATAIVGTLGYVVFFRWSLIDAAYMTVITLTTVGFREVRELVDWPQRL
                     WTMLVAIAGVGIIYGSIGIVAEAVLAEVSSGRREARQMAEALADLEGHYILCGYGRVG
                     STVARELVHAGYRIVVIDILPASLERARSDGHLVVEGDATTDATLATARIDRARGLVA
                     TIDSDALNVYVTLSARALNARLFIVARANAEGSEAKLAQAGANRVVSPYTMAGRRIAE
                     LAVRPRVADFIDAALSHGELRFSMEELEIAAGGPLDGRTVGQLREEGVFTLAVVRGDT
                     EYDPNPPPDRVLVAGESLVVSGSTERLSVLRDRA"
     gene            10441..11934
                     /locus_tag="VHM48_05050"
     CDS             10441..11934
                     /locus_tag="VHM48_05050"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2754806.1"
                     /translation="MTTEQRSPDGAATDAPTTARMMIGGESVDAADGQTFDITNPATG
                     KVIATAPLGGREDVDRAVAAARKAFDDRKGWANWAAGKRGRTLAKFADLIKRNSEELA
                     WLESRNVGKPIGGARGEIIGASLVFDYYAGAANKVYGQTIPVSKPGLDLTLREPIGVV
                     GLIVPWNFPLLMASWKVAPALAAGNTAILKPASYSPLTAIRLGELALEAGLPAGVLNV
                     VTGPGGTAGASIAAHPGVGKVAFTGETTTGQEIMRLASGNVKKISLELGGKSPNIVFA
                     DADLEKFARESPYSVFDNCGQDCCARSRILVERSVHDQVVELFAAATRNVKVGDPADE
                     ATEVGTLVSAKQRDRVADYIEIGQAEGATIVVGGAPPDDPALADGAYLMPTVFDNVRN
                     DMRIAAEEIFGPVVSIISFDTEEEALRLANATPYGLAGSIWTRDIGKALRSAKALQSG
                     VISVNSNSSVHTEAPFGGYKMSGIGRELGMSALDLYTEIKNVFIDIS"
     gene            complement(12004..>12505)
                     /locus_tag="VHM48_05055"
     CDS             complement(12004..>12505)
                     /locus_tag="VHM48_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2754807.1"
                     /translation="LTFGSIDGEDRDRLGCPACGYIAYVNPRLVVTTIPVTDAGEIVL
                     LRRGIDPGRGWWAQPGGFLEVDETVGEAAIRETLEETGLIVEPGEIVGLYSRLEAAVV
                     VLAFEARIVGGSYRLNPEALEIAAFAPDAIPWPGIAFKTSYWAIRDWLRRRRPDIHPA
                     DRHWDE"
BASE COUNT         1911 a         4499 c         4288 g         1807 t
ORIGIN      
        1 ctcgcggcca gcctcagcgt cgccctcgaa aacgtgcgcc tgttcgacga gacgaagcgc
       61 ctcctgtccg agacgaacga acgcgccgcc gagctggccc tgatcaacga cgtccagcac
      121 ggtctggccc agaagctcga catgcaggcg atgtacgacc tcgtcggcga ccggatccag
      181 gcgatcttcg acgcccaggt cgtggacatc ggggtgatcg accgcgagca ggagctcgtt
      241 cggttcccgt actggatcga gcgcggggtc cggttcccgg acgagccgat gccgctcgtc
      301 ggcccgcgtc gtcagatcat ggagacccgc cggccgatcg tcgtcaaccg cgacatgcgt
      361 gcccgcatga aggagcttgg ccaggacctg actctgcccg gcgaggtgcc ccaatccgcc
      421 ctgtgggcgc cgctcgtcgt cggtgacgag acccgcggcg tgatctcgct ccagaacctg
      481 gatcgtgaag acgcattcag cgactccgac gtcgagctcc tgacaaccct tgccgcgagc
      541 ctcagtgtgg ccctcgagaa cgtccgcctg atcgacgaga cccgccaccg actggccgag
      601 ctggccacgg tcaacgaggt cggcaaggcg ctcgcgagcc agctcgacct cgacaacctg
      661 atccagctcg tcggcgacca gatgcggcgg accttcgcgg cggacatcac gtatgtcgcc
      721 ctccacgacg tcgagagcca gcgaatcgac ttcccctact acaacgagtt cggcagcgcc
      781 accggccagg atgcgatccc cttcggcaaa ggcctgacct cgaagatcat cgatacctgc
      841 gagacgctcc tcctgaaccg ggaggccgat tgggacaacc tcggcacgcg cggcgtcggg
      901 acgctctcga aatcctttct cggcgttccg atcgtcctcg gcgaccgggc catcggggcg
      961 atcagcgtcc agagcaccac ccaggagggc cgcttcggcg aatctgacgc acgactcctc
     1021 tcgacgatcg ccgccaatgt cggtgtcgcc atccagaacg cccgcctgta tcgcgaggcg
     1081 catcgtcgcg gcgacgagat ggccgccctc gccgacgtcg gccaggagat ctcggcgacg
     1141 ctcgatgccg atgtcgtcct gcgccagatc ggcgagcgag tccagtcgct gctcgatgcc
     1201 gatgcctcgg ccctcttcct cgccgatccg gatggacgaa gtttccggcc gatcctcgcc
     1261 ctcggcacga tcgccgagca gatcctcgcc gacacgatcg tcgaaggcca ggggatcatc
     1321 ggcgacgtca tccggacccg gacgccggag tacatcaacg atgtcggcgc cgatgcccgg
     1381 acggtcacga tcccgggaac ggaggacatc gccgaggagc ggttgatggt cgctccgctg
     1441 gtcgcccgcg accgcgtgag cggcgtcatg gcggtctggc gcaaccacgg cgcaccgttc
     1501 agccaggccg acctcgactt cctcgtcggg ctcgctcgcc aggcctcgat cgcgatcgag
     1561 aactcgcgac tgttcgccga ggccggcgtt gcccgggccg cggccgagga cgccaaccag
     1621 gccaagagcg ctttcctcgc cgcgatgagc cacgagatcc ggacgccgat gaacgcggtc
     1681 atcgggatga gcgggctcct cctcgagacc gagctcgacg acgagcagcg ggacttcgcc
     1741 gacacgatcc gcagcagcgg cgacgcgctc ctgacgatca tcaacgacat cctcgacttc
     1801 tcgaagatcg aggcgggccg ggtcgacctc gttgccgagc cgttctcacc cagggtcgcg
     1861 gtcgagagcg ccctcgacgt catcgccccg acggcggcgc ggaagaaggt cgagctcgtc
     1921 tacgcgatgg gcgacggact acccgacgcg atcgttggtg acgcgggtcg gctccgccag
     1981 atcgtcctga acctcctctc gaacgcggtc aagttcaccg accagggaga ggtcgtcctg
     2041 ggcctcgagg ccacacgggg gacggccgct ggcgatccgt ggacgctcgc gatcgaggtc
     2101 cgcgacaccg gcatcggcat cccgccggac gcgatggacc gcctgttcca gtcgttcagc
     2161 caggtcgacg cgtcgatctc gcggcggttc ggcgggaccg gactcggact cgcgatcagc
     2221 cgccggctgg ccgaatcgat gggcggcgcc ctgaccgcca ccagcagcgg cgtcgccggc
     2281 gagggcagca cgttccgcct gaccctgccc gtcgaggtga cggtcctccc ggacgccgtc
     2341 gcgccgccac cgatccggag cctgcgcggc tgccgggtgc tcgtcgtcga cgacaacgcg
     2401 acgaacagga agatcctgac cacgcttctc caacgctggg aggtcgacgc cgccgcgacc
     2461 gcatcgccgc tcgaggcgat cggctgggtc cgcgctggcc agacgttcga tgtcgcggtc
     2521 ctcgacctgc tgatgcccga gcgtgacggg atcgagctcg ccggcgacct gcgcgacctg
     2581 cggccggaca tgccgatccc ggtcgtcatc ctgtcgtcga tcggccagca ttcccgaacg
     2641 gccccgaaca tccgggcgat gctggtcaag ccggtcaagc cgtcggcgct ccacgatgcg
     2701 ctcgcgaccg ccgtctccgg cgaggaggtc gtggcccggc cggatcgggc ggcgtcggcc
     2761 tcgccggcag cggcggcggc gcggaacggc aacctccgga tccttctcgc cgaggacaat
     2821 gcggtgaacc agaagctggc gttacgcctg ctcgagcgga tgggcaacgg cggcgccgat
     2881 gtcgtcgagg acggagaggc cgtgatcgag gcgctcgaga agaccgacta cgacctgatc
     2941 ctgatggacg tccagatgcc gcgcatggat ggcctcgagg ccacccgccg cgtccgcgcc
     3001 cggtggcccc agcgggcggt gcggatcgtc ggcctgacgg cgaacgccat ggccggcgat
     3061 cgcgaagcct gtctcgaggc aggcatggac gactacgttt ccaagccgat ccggcccgac
     3121 gagctcgccc gagcgatcgg ggcgacgagc cgcggcccgg tcgcgtccgg atccagttcc
     3181 gacgagccac ggggaacgag ctcgtgagcg agctcgagcc gcgcagcgcg ggcgaggccc
     3241 ccggcgtcat ggacccgtct ggtccggcgg aggcgctcga ccacgacgcg atgaccgggc
     3301 gagtcctcgt cgtcgacgac acgccgttca accgtcagct gctgacccgg ctcctcggcc
     3361 ggatcgggca cacgtcgctc gaggcggccg atgggcaggc cgccctggac ctgctccggg
     3421 cgcccgccac cgagccggtc gacgtcatcc tcctcgacat catgatgccg gtcatggacg
     3481 gctacgagac gcttgccgcc ctcaaggccg acaagaccct gggcgacatc ccggtcatcg
     3541 tcatctccgg tgtcgacgag ctcgacagcg tcgtccgctg catcgagatg ggcgcggctg
     3601 actacctgcc gaagaccgtc gacccggcga tcctgcgggc gcggatcgcc gcctcgctcg
     3661 gccagaaacg gttgcgcgac caggagcgtg cgctcctcgg gacgatcgat cgccagcggg
     3721 cccagctcgc ccggttcctg tcgccgcagg ttgccgctct cgtctccagc cccgacggcg
     3781 aggcgatgct ggccggccac cgccgcgaga tcaccgcgat gttctgcgac ctgcggaact
     3841 tcaccgtgtt ctcggagacc gcagagcccg aggaggtcct cggattcctg cggctctacc
     3901 acgcgaccct cggcgccctg gtcgtcgccc acgagggcac cctggagcat ttcgcgggcg
     3961 acgggatcat ggtcttcttc aacgacccgg tcctccagac cgaccacgcc cagcggtcgg
     4021 tcgagctcgc cctcgacatc cgcgagcggt tcaaggacat ctccgcggag tggcgccgac
     4081 gcggtcacgt cctcgaggtc gggatcggga tcgcgaccgg ctacgcgacg atgggccgga
     4141 tcgggttcga gggccgctac gactacggcg gcgtcggcaa cgcgatcatc ctggcctcgc
     4201 gcctgagcgg cgaggcgacc gccggccaga tcctgctcgc cgccaggacg tacgcggcga
     4261 tcgacgaggc gctcgccgag cccgtcggcg accgccagct caagggcttc agccggccga
     4321 tgccggtcta tgccgccggg ccgtccgacc ggacggcctc caccggcgcc gacgcggccg
     4381 aggtctttgg atgaccgggc cgatcgacga cgcggtcttc gccagcctcg tcgagatgac
     4441 cggcggcgag atggacttcg tcgacgagct cgtcgacacg tacctcgagg acggcgcgaa
     4501 ccagatcgcg acgatgcggg ccgccgtcga aagcggcgat gcggaggggc tcacgcgggc
     4561 cgcccactcg ctcaagtcgg gcagcctgaa cgtcggcgcg ctggagctcg gcgggctctg
     4621 ccgggccctg gaggaagcgg gccggagcgg ggcgatcgcc gatcccgacg cccggatcgc
     4681 ggcgatcgac gacgcgttcg cggcggccgg cacgacgctc ctggacgagc gagggcggcg
     4741 gacccccggc tgaggatccg ccacccggcc ggccgggctg gccgacactg ctcgtggctc
     4801 ggcgacggct gaccgcgagt accatcagcg cacgttgcgc cccggcgcga cccgtcccgg
     4861 cgacccggcc atcgcttcgg ccgcgtccgc ccggcacgcc cagaggaagg atccgtcgcc
     4921 cgtgcgtctc gaggacaaag tcacgatcat caccggcgcc ggtggcggca tggggcggac
     4981 agccgcccgg atgttcgccg ccgagggagc gcgcgtcgtc tgcgtcgacg tcaccgagga
     5041 gatggccgag tcggccgccg ccgacgtcac cgccgcgggc ggccaggcaa cggcgatcgc
     5101 cgcggatgtc tcgcaggacg gccaggcaaa gcggatggtc gaccacgcga tcgccacgta
     5161 cggccgcgtg gacgccctgt acaacaacgc cgggatcatg cccgcggccg accactcggt
     5221 catcgacacc gacgtcgaga cgtgggacaa ggtcatggcc gtcaacgtcc ggggcgtcta
     5281 cctcggctgc aagtacgcga tcccgaagat gatcgagcag ggctcaggct cgatcatcaa
     5341 cgtcgcctcg ttcgtcgcga tcctcggctg ctcggtgccg caggatgcct acaccgcctc
     5401 gaagggtgcg gtcctcgccc tgacgaggag cctcgccgtc cagttcgcgc cgcagggcgt
     5461 tcgctcgaac tcgatctcgc ccggaccgat cgagacgccg ctcctgatgg actggctcgt
     5521 caaggacgag gaggcgaaga agattcgcct ggcgcgcaac ccgaccggac ggttcggccg
     5581 acccgaggag atcgtccatg tggcgctcta cctcgcctcc gacgagagcc gctggacgaa
     5641 cggggccaac ctcgtgatcg acggcggcat ctccgtcaac tacttctgag gacgggccgg
     5701 atggaccaag cccaggggac ccgcgttgcg gtcggggtcg atgtcggcgg gagcgggatc
     5761 aaggtcgccg tggtcgacgt cgaccgcggc gtcctcgtcg ggccgcggct gcgggtcgcc
     5821 acgccgcagc cctcgacgcc ggacgtcgtc gtcccggtca tcgtccggac cgtgcgccgc
     5881 gccctgcgtg aggcgaaggt cgatcgagcc gacgtgacgg tcggcgtcgg gatgcccgcg
     5941 gtcgtcttcg acggtgtgac gaagtcggcc gcgaacatcg acccgctgtg ggtcgatttt
     6001 gacctcggat cccacctccg ccaggcattg aagcgatctg tcttcgtcct caacgacgcc
     6061 gacgcagccg gcctcgccga gatgcgcttc ggggccggcg tgggggagcg cggcgtcgtc
     6121 ttcgtcctga ccctcggcac cggcctcggc tcggggatgt tcaacgatgg ggtgctcgtc
     6181 ccgaacaccg agctcggcca catggagatc cgcggtcggg acgccgaacg ccgctcggcg
     6241 gccgtcgctc ggacgcggcg cggcctgtcc tggaaggcgt gggcgagcga cctggacgag
     6301 cacctccacg caatcgacaa gctgttctcg ccgaacctga tcatcctcgg cggcggcgtg
     6361 agcaagaacg cggacaagtt catcccccgg ctcacggtcc ggccgcgtgt cgtgccggcg
     6421 acgctccgca acgacgccgg aatcgtcggt gcggcgatgg ccgcgtccga gaagttcatc
     6481 gagggcgccg cctcgacgcg ctgacccgca gccctggccg gaacgccggt cgtgccggat
     6541 cgatcgtacg gcgtcgtccg taggccaccg tccacccatt ggcgcaccgc cgtcgctctc
     6601 ccgggcgatg acacccgcgc tcgccagcga cgacgattgc gccagcgtcc tgtgtcgggc
     6661 gtgactcccg cgagcggtga acatccagcg tggaacgatc gacgatgtcg gaaacgaaga
     6721 gccgacccgc gtgggcgtgg cgcgtcttcg ccgtggccgc gcccgtcttc gccttgctgt
     6781 acctcgcgct tcccggcagc gggccggccc ggaccctggc gtatccagtc ttcggaatcg
     6841 tcgcctcgat cgcgatcgtc gtcggcatcc ggcggggatc cccgacccgg cccgggtcgt
     6901 ggtggctgat cgccggcgcc ctcgtcctga tggcaacggg cgacatcgcc tactccgcga
     6961 tcgagctcgt cggggaggtg ccctatccgt ccctcgccga cgcgttctac ctcgccgggt
     7021 accttgccct ggtcggaggc gtgaccggtc tggcccgcgg ccggttgagt ggcggcgacc
     7081 ggacgccgct catcgacggc gcgatcctgg cggccggcgc cgcatccatc ctgtggatcg
     7141 tggtcatgcg accgagcatc gtcggccagg tcgacgtgct cgcggcgacc gtctccctcg
     7201 cctatccgtg catggatctg gtcattctcg ccctggcgtt ccgtgtcgtc ctgtccggcg
     7261 atcgaccggc ctacctcaac ctcctcctgg cgggcctggc gatctacttc gccgccgaca
     7321 tcatctacgc gatggcggtc ctcgacggga cctatgtcga cggtcatccc gtcgatgccc
     7381 tgtggatcat cggtgtcacg ttgtggggtg cggcagccct tcatccgtcg atcgcgcatc
     7441 ccgtccgcct cgtcgtcgag gaggaccagt acctcagccg gtcccgtctg gccctgcttg
     7501 ccctcgcctc gctgatcgcc ccagcgacgt tgacggtcca ggccgccatg gccggcgagg
     7561 gcgacgccat cggcctgatc atcgcctgga cgatcctgtt cggcctggtc ctgatccggc
     7621 tcgcctcgac cgtcgacgag ctcggcggtt cgctcctcca gcgccggcgg ctcgaggacg
     7681 acctgaccca ccaggcccag cacgacccct tgaccgggtt ggcgaatcgg tcgctcttcg
     7741 aacgacgcct gacaaccgcg atcgaccgcc gtcccgacac gacggcattg gtcttcctcg
     7801 atctggacga cttcaagacg atcaacgaca ccctgggcca cggcgtcggc gatgagctgt
     7861 tgcgaatcct cgcagaccgc gtccgcggcg agttgcggac cggcgatctc gcggcccggc
     7921 tcggaggcga cgagttcgcc gtcctcgtcg agcactgccc ggacgagccg accgtacggg
     7981 ccatcgccga gcggatcctc gcggcgatgc gcaagccgct cgtgctcggt ggacgacagc
     8041 tccaggtccg cggcagtgcc ggcgtcgcag tcggacgcgt cgagaccacg ccgatggatc
     8101 tcatgcgcaa cgccgacgtc gcgatgtacc aggccaagct ccacggcaag gaccaggtcg
     8161 agacgtacga gccccggatg cacagccagg tcgtccgggg ctaccagctc cggaccgagc
     8221 tggccgaggc gatccgccgg cagcagttcg tgttgcacta ccagcccgtc ctgaggctgg
     8281 cgaccgacga gattgtcggt gcggaagccc tcgtccgctg gcgtcatccc gagcgcggcc
     8341 tcgtgggccc ggacgatttc atcccccagg cggagtcgag cggcctcatc cacgacctcg
     8401 gccgttggat ccttcgggag gcctgtgcaa ccgcggcggc ctggccgctc cggccggacg
     8461 gaacggcggc atcggtcagc gtcaatctcg cgccgagcca gctcctggat ccgaagctcg
     8521 tcgacgatgt cgcgagcgtc ctcgccgcga cggggctccc ggccagtcgc ctctacctcg
     8581 agatcaccga gagcgccctc gtcgacctcg atcgagcgtg cacagcgctg gagggcctgc
     8641 gcgggctcgg gatcggcctc gccctcgacg acttcgggac gggctattcc gccttgagcc
     8701 acctggcgtc gctgccgttc ggcatcgtga agatcgaccg cggcttcatc cgctcggccc
     8761 gcgagggaac gcgggtcggc aatctgctcg ccgggatcgt cggcctctgc cacgcgctcg
     8821 acctgctcac cgttgccgaa ggcatcgaga ccgaggacga ccttcggctc gtccgccgcg
     8881 tcggctttga tgtcggtcag gggtatctct tcgcgaagcc gatcccgggt ccggagttcg
     8941 ctgcgatcta tgccagcggc aggctcgacc ggccccgccc cgagggacaa cggccgacga
     9001 cgatcggacg cgtcgtggag ccgacggcgg cataggcctc aggcacggtc acggagcacc
     9061 gagaggcgct cggtcgagcc ggacaccacg agcgattcgc cggcgacgag cacgcggtcg
     9121 ggtggcggat tggggtcgta ctcggtatcg ccgcggacga ccgccagggt gaagaccccc
     9181 tcctcccgga gctggccgac cgtgcgtcca tcgagcggtc cgccggcggc gatctccagc
     9241 tcctccatcg agaaccgcag ttcgccgtgg gacagggcgg cgtcgatgaa gtcggcgacc
     9301 cgcgggcgga cggcgagctc ggcgatccgc cggccggcca tcgtgtacgg cgagacgacc
     9361 cgattggcgc cggcctgggc gagcttcgcc tcggagcctt cggcgttcgc ccgggcgacg
     9421 atgaacagcc gggcgttgag ggcgcgcgcc gacagcgtca cgtagacgtt caatgcgtcc
     9481 gagtcgatcg tcgcgacgag gccccgggcg cggtcgatcc gggccgtcgc gagggtcgcg
     9541 tcggtcgtgg cgtcaccctc gacgacgaga tggccgtcgc tgcgggcccg ctccagggag
     9601 gccggcagga tgtcgatgac cacgatccgg tagccggcgt ggaccagctc gcgggcgacc
     9661 gtggagccga cccggccata gccgcacagg atgtagtggc cctcaaggtc cgccagtgcc
     9721 tccgccatct gccttgcctc ccgtcgaccg ctcgagacct ccgcaaggac ggcttccgcg
     9781 acgatcccga tgctgccgta gatgatcccg acgccggcga tcgcgacaag catggtccac
     9841 agtcgttgcg gccaatcgac gagctcgcgg acctcgcgga atccgaccgt cgtcagcgtg
     9901 atcaccgtca tgtacgcggc gtcgatcaag ctccaccgga agaagacgac gtagccgagc
     9961 gtcccgacga tcgcggtggc cgcgatgatc gcaagccaga tccggatatg ccgtccctgg
    10021 acgacctccg cgacgttccg gacccagcgt cgacccgaac ggccccgccg gccacgaaca
    10081 gcgggcgcgg ggcgcaccgg atcggacgca agggatccat cgcgacggaa cgagggtgca
    10141 gtcatcggcc gaagtatcca tcggcgaacg tcgccgactc cgggccatcc ggcacgtccc
    10201 tgcccggacc gctggcacga accgctgacg gcgccctcgc cgccgcgcgg taccgacgcg
    10261 agccggagcg tcggcggcgc cgcagcggcc acgttcggcc ccggaagggc tgatccccgc
    10321 ttcttgccgg cgagccccgg tctggtggat cccgaccggg gctcgtcgat tccgggcgta
    10381 ccatctgcgc acgaaccgcg gccgtccgcc gccatcaacc cgaccacgag gacgcgaccg
    10441 atgacgaccg agcagcgctc gcccgacggg gccgcgaccg acgccccgac gaccgcccgg
    10501 atgatgatcg gcggcgagtc cgtcgacgcg gccgatggcc agacgttcga catcacgaac
    10561 ccggccacgg gcaaggtcat cgccaccgcg ccgctcggtg gccgcgagga tgtcgaccgg
    10621 gccgtcgccg ctgcccggaa ggcgttcgac gaccgcaagg gctgggccaa ctgggcggcc
    10681 ggcaagcgcg gccggacgct ggccaagttc gccgacctga tcaagaggaa cagcgaggag
    10741 ctggcctggc tcgagagccg caacgtcggc aaaccgatcg gcggagcgcg cggcgagatc
    10801 atcggcgcga gcctcgtctt cgactactac gccggcgccg cgaacaaggt ctacggccag
    10861 acgatcccgg tctcgaagcc cgggctcgac ctcaccctgc gcgagccgat cggcgtcgtc
    10921 gggttgatcg tgccgtggaa cttcccgctc ctgatggcct cctggaaggt cgcccccgcc
    10981 ctcgccgcag gcaacaccgc gatcctcaag cccgcgtcgt acagcccgct gacggcgatc
    11041 cggctcggcg agctggcgct ggaggccggg ctgccggccg gggtcctgaa cgtcgtgacc
    11101 ggccctggtg gcaccgccgg agcgtcgatc gccgcccacc ccggcgtcgg caaggtcgcc
    11161 ttcaccggcg agacgacgac cggtcaggag atcatgcggc tggcgtcggg taacgtgaag
    11221 aagatcagcc tcgagctcgg gggcaagagc ccgaacatcg tcttcgccga tgccgacctc
    11281 gagaagttcg cccgcgaatc gccctactcc gtcttcgaca actgcggcca ggactgctgc
    11341 gcccggagcc ggatcctcgt cgagcgctcg gtccacgacc aggtcgtcga gctgttcgcc
    11401 gcggcgacgc ggaacgtcaa ggtcggcgat ccggccgacg aggcgaccga ggtcgggacg
    11461 ctcgtcagcg cgaagcagcg cgaccgggtc gcggactaca tcgagatcgg ccaggccgag
    11521 ggcgcgacga tcgtcgtggg cggcgcgccg cccgatgatc cggcgctggc cgacggcgcg
    11581 tacctgatgc cgaccgtgtt cgacaacgtg cgcaacgaca tgcggatcgc ggccgaggag
    11641 atcttcggcc cggtcgtgtc gatcatctcg ttcgacaccg aggaggaggc cctccgcctg
    11701 gccaacgcca cgccgtacgg cctggcgggc tcgatctgga cccgcgacat cggcaaggcg
    11761 ctgcgctcgg caaaggcgct ccagtccggc gtgatctcgg tcaactcgaa cagctcggtc
    11821 cacaccgagg cgccgttcgg cggctacaag atgtcgggca tcgggcgcga gctcgggatg
    11881 tccgcgctcg atctctacac cgagatcaag aacgtcttca tcgacatctc gtgacgggct
    11941 cggggcactg gtcgatgacc tgcccgccta cgtgaccggc ggcggttgac gctcgggctg
    12001 cggctactcg tcccagtggc gatcggccgg gtggatgtcg gggcgacggc ggcggagcca
    12061 gtcgcggatc gcccagtagc tcgtcttgaa cgcgatgccc ggccacggga tcgcatcggg
    12121 ggcgaaggcc gcgatctcga gggcttcggg gttcaggcgg tacgacccgc cgacgatgcg
    12181 cgcttcgaag gcgaggacga cgaccgcggc ctcgagccgc gagtacagcc cgacgatctc
    12241 gcccggctcg acgatcaggc cggtctcctc gagcgtctcg cggatcgccg cctcgccgac
    12301 cgtctcgtcg acctcgagga agccgccggg ctgggcccac cagccgcgac cggggtcgat
    12361 gccacggcgc aggaggacga tctcgccggc gtcggtgacc gggatcgtcg tcacgacgag
    12421 acgcgggttg acgtaggcga tgtagccgca ggccggacag ccgagacggt cgcggtcctc
    12481 accgtcgatc gagccgaacg tcagc
//
LOCUS       DATAIW010000158         5417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_37907, whole genome shotgun sequence.
ACCESSION   DATAIW010000158 DATAIW010000000
VERSION     DATAIW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37907"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1350)
                     /locus_tag="VHM48_05060"
     CDS             complement(97..1350)
                     /locus_tag="VHM48_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2754808.1"
                     /translation="MSPSDPSPSDAFETLAVHAGAEPDEVTGAVSPPIYQTSTYAQDG
                     VNRPRRGHDYARSINPTRDRLERAVAALEKGTHGIAFASGSAATAAIAQLAASGEEIV
                     VGDDVYGGTYRYLERVHRPGGAADARYVDLSAGSDVLWEQLTARTRLVWFESPSNPNL
                     KLTDIAAAATTIRDRAAQTGATRPYLVVDNTFASPALQNPLELGADIVFHSATKYLGG
                     HSDTILGIAVTRDDAIAERLHFLQNAMGGVPGPLDCFLVLRGLRTLHLRVERHVANAT
                     AIARFLAARDDVERVFYPGLAAGPHAHPGAALVGEGRQMRGGGGMVSFIPRPGGPGGA
                     AGDGHGARSAADRAIAIAEATRLFTLAESLGGVESLVEIPATMTHASVAGSKLEVDPA
                     LVRLSVGIEHVDDLIRDLGNALDHA"
     gene            1407..1754
                     /locus_tag="VHM48_05065"
     CDS             1407..1754
                     /locus_tag="VHM48_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754809.1"
                     /translation="MKEHVFGCPYQSRPAVLMIAFYPIVLAAVVVGWIGPWIALVVLG
                     IPRLLEVLKTFAAPRPATPPHSYVGWPLWFVGAAFIHTRRAGGLLVLGLLLNAILPIK
                     LPWIAQRGGRRRR"
     gene            1801..2118
                     /locus_tag="VHM48_05070"
     CDS             1801..2118
                     /locus_tag="VHM48_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2754810.1"
                     /translation="MPLRTGRGIALLADPTRRRIIAALATKPRRPSRLASDLAVSRPA
                     VARQLRLLEEAELIVAHEVAGDGRGILYTINPRRHGAITAWLAGTEVGRESAQGDRPV
                     REA"
     gene            complement(2505..3920)
                     /locus_tag="VHM48_05075"
     CDS             complement(2505..3920)
                     /locus_tag="VHM48_05075"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX2754811.1"
                     /translation="MHYAETILDLVGHTPLVRLSRVTRDLGPIDRQPLILAKLEMLNP
                     GGSVKDRIGLPMIEAAEKAGLLKPGGTIIEPTSGNTGHGLAIAAVLKGYRCIFVMADK
                     QSTEKQSLLRAYGAEVVLCPTNVDPESPESYYSVAARLARDIPGGFKPDQYWNMENPI
                     AHERTTGPEIWEQTEGELTHFVASVGTGGTISGAGHFLRRQKPDLVIVGADPEGSVLS
                     GDNARPYLTEGVGEDFFPGTYDPATVDRWVRVGDREAFAAARRLTREEGILAGGSCGT
                     ALVAALTVARELVAAGEGAGKIMVVLLPDSGRNYLSKVYNDEWMRVNGLLPTTGGVAR
                     VADLLSRRQHDPERPVVVVARTTQRVGEAIATLQEYGISQMPVSEDASGDGVAGLVGS
                     ISERSLLEKAYRDPTIVERTVGEAMDGPLPLVDSSATLDEAFALLSSGSPAVVAVRGE
                     QPAGVVTKLDLLEYLSHLSRG"
     gene            complement(4018..>5417)
                     /locus_tag="VHM48_05080"
     CDS             complement(4018..>5417)
                     /locus_tag="VHM48_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2754812.1"
                     /translation="GSHPLLIYLLATITATLVALSRPIHSALMPEVVTSPDDLTAANV
                     VSGMGESAGSLIGPLGAGFVLGLGGPAAVFVVAAVGNVVGSLAVLGVVRRSSFLRRGD
                     AAAFAPVGGSVPDAVDAAPRIDRWRSTASELIGGVSAILADDRLRAVVLIATWGTFLV
                     GAMDILYAVLAIELMGLDGGGVGFVGAIGGVGAILGSVAGLLLVGRERLGLSLAASAV
                     LFGAGIATIAIATGSFAAAALLVLAGIGSGLTNVGAQTLIHRLAGDDVMSRVFGVLQG
                     MMMGATALGALAVPLAITLVGNRAAFAVAGLSLPVVLVLVGSAIVRGDRLDVGRAAEL
                     RLLRGVPMLAPLSGPVLERLAGGIVRSHHSRGTTVIREGDPGDRFYVVETGALGVSVM
                     GREARRLGPGDGFGEIALVRDVPRTATVTALDDVVLLGIDRGPFVEALGGQARSRTIA
                     AVLVDDRLASDVTVS"
BASE COUNT          806 a         1906 c         1956 g          749 t
ORIGIN      
        1 gtcctgggac gtgtcagctg tgtgtcaggt tcacggggcc gggcgcggac gcctttccgt
       61 tcggtgacga ggcgtcgccg atgccgggct gggcggtcag gcgtggtcga gggcgttccc
      121 gaggtcgcga atcaggtcgt cgacgtgctc gatgccgacc gagaggcgaa ctagggcggg
      181 gtcgacctcg agcttcgaac cggcgaccga cgcgtgggtc atcgtcgccg ggatctcgac
      241 gagcgactcc acccctccga gggattcggc gagggtgaag agccgggtgg cctcggcgat
      301 cgcgatcgcc cgatcagcag cggatcgtgc gccgtgaccg tcgccggccg ccccgccggg
      361 cccgcccggc cgagggatga acgagaccat cccgccgcca ccgcgcatct gacggccctc
      421 ccccaccagg gccgcgcccg gatgggcgtg cggccccgcg gccagccccg gatagaagac
      481 ccgctccacg tcgtcacgcg ccgccaggaa ccgcgcgatc gcggtggcat tggcgacgtg
      541 ccgttcgacg cggaggtgga gggtccgcag cccgcgcagg acgaggaagc agtcgagcgg
      601 cccgggaacg ccgcccatcg cgttctggag gaagtggagc cgttccgcga tcgcgtcgtc
      661 gcgggtgacg gcgatcccga ggatcgtgtc ggaatggccg cccaggtact tcgtggccga
      721 gtggaacacg atgtcggccc cgagctcgag cgggttctgg agggccggtg aggcaaaggt
      781 gttgtcgacg acgaggtacg gccgggtggc accggtctgc gcggcgcggt cccggatcgt
      841 cgtggcggct gcggcgatgt cggtgagctt gaggttcggg ttcgacgggc tctcgaacca
      901 gaccagccgc gttctcgcgg tcagctgctc ccacaggacg tccgatccgg ccgacagatc
      961 gacgtagcgc gcgtccgcag ctccgcccgg tcgatggacc cgctccagat agcggtaggt
     1021 cccgccgtag acgtcgtcgc cgacgacgat ctcctcgccg ctcgcggcca gctgggcgat
     1081 cgcggcggtc gccgcggacc cgctggcgaa cgcgatcccg tgcgtaccct tctcgagcgc
     1141 cgcgaccgcc cgctcgaggc gatcgcgggt cggattgatg ctccgggcgt agtcgtggcc
     1201 gcggcgcggc cggttgacgc cgtcctgggc gtaggtcgag gtctggtaga tcggtggcga
     1261 aacggctccg gtgacctcgt cgggctcggc cccggcgtgg acggcgaggg tctcgaaggc
     1321 atcggacggg gagggatcgg aagggctcat cgacccaatg atgtcatcca acgggccggc
     1381 gccgtcgcat ccatgaacgt gctagcgtga aggaacacgt tttcgggtgt ccctaccagt
     1441 ctcgccccgc ggtcctgatg atcgccttct acccgatcgt gctggcggcg gtggtcgtcg
     1501 gctggatcgg gccatggatc gccctcgttg tcctcgggat ccctcgactg ctcgaggtcc
     1561 tcaagacgtt cgccgcacca cgaccggcga ccccgccgca cagctacgtc ggctggccac
     1621 tctggttcgt cggcgcggcg ttcatccaca cccgccgggc cggcggcctg ctcgtcctgg
     1681 gcctgctcct caacgcgatc ctgccgatca agctgccctg gatcgcgcaa cgcggaggcc
     1741 gacgtcggcg ctgaacctgg cgttgaccga caggtaagcg tcgtcgggca ttgtcgttgg
     1801 atgccactcc gaaccggccg cgggatcgct ctcctggcag acccgaccag gcggcggatc
     1861 atcgcggcgc tggccacgaa gccgcggcgc ccgtcgcgtc tcgccagtga ccttgccgtc
     1921 agtcgaccgg ccgtcgcgcg ccagctccgc ctcctcgagg aggcggagct gatcgttgca
     1981 catgaggtcg cgggcgacgg tcgcggaatc ctgtacacca tcaacccacg tcgacatggc
     2041 gcgatcacgg cgtggcttgc gggaacggag gtcggccgcg agtccgccca gggcgaccgg
     2101 ccggttcgcg aagcctgaca agaatggccc cccagcgcgt cgatttcggg gtcgttcacg
     2161 ccatgcggaa cgactccggg cctgcggccc gcgaccgggc tcggccgccg tgaagggtct
     2221 cccagcgcgt ccatttcggg gtcgttcacg ccatgcggaa cgactccggc gcggcggcgc
     2281 cacagcggcg tcaccacagc ggcgtcacca cagcggcgtc accacagctg tggcgggagc
     2341 cgagcgatcc ggccggcaca cgccctcgag atggttccgc ttgggcgcaa cgactccgaa
     2401 tcggtcgccc tgggcgacgt ttcatccgca agcgttcggc cgtgtccaag gcctgggccg
     2461 cgggagtctc gcgttcggac ggacggccag gcgccgcgcc gggctcagcc gcgcgacagg
     2521 tgcgagaggt actccagcag gtcgagcttg gtgacgacgc cggccggttg ctcgccacgg
     2581 acggcgacga cggccggcga accgctgctc agcagggcga aggcctcgtc gagcgtggcc
     2641 gacgagtcga ccagcggcaa tggcccgtcc atcgcctcgc cgaccgtccg ctcgacgatc
     2701 gtcgggtcgc ggtaggcctt ttcgagcagc gaccgctcgc tgatcgagcc gacaagaccg
     2761 gcgaccccgt cgccggaggc gtcctccgac accggcatct ggctgatccc gtactcctgg
     2821 agggtggcaa tcgcctcgcc gacccgctgc gtcgtccggg cgaccaccac gaccggccgc
     2881 tccggatcgt gctgtcgccg ggaaagcagg tccgcgaccc gggcgacccc gccggtcgtg
     2941 gggagcaggc cgttgacccg catccactcg tcgttgtaga ccttcgagag gtagttccgg
     3001 ccgctgtccg gcaggaggac gaccatgatc ttccccgcgc cctcgccggc agcgacgagc
     3061 tcgcgggcga ccgtcagcgc ggcgacgagc gcggtcccgc acgagccgcc cgcgaggatt
     3121 ccctcctcgc gcgtcaggcg gcgggcggcg gcgaacgctt cgcggtcgcc cacccggacc
     3181 cagcgatcga ccgtggccgg gtcgtaggtg ccagggaaga agtcctcgcc gacgccctcg
     3241 gtcagatacg gccgtgcgtt gtcccccgac aacacgctgc cctccggatc cgcgccgacg
     3301 atgacgagat cgggcttctg ccggcggagg aaatggcctg ccccggagat cgttccgccg
     3361 gttccgacgc tggcgacgaa atgggtcagc tccccctcgg tctgctccca gatctccggg
     3421 ccggtcgtcc gctcgtgggc aatcgggttc tccatgttcc agtactggtc cggcttgaac
     3481 ccgcccggaa tgtcgcgtgc gaggcgggcc gcgaccgagt agtacgactc cggcgattcc
     3541 ggatcgacat tggtcggaca taggacgact tccgcgccgt acgcccggag cagggactgc
     3601 ttctcggtcg attgcttgtc ggccatgacg aagatgcagc gatagccctt gagcaccgcg
     3661 gcgattgcga ggccgtgacc ggtgttgcca gacgtcggct cgatgatcgt cccgccgggc
     3721 ttgagcagcc cggccttctc ggccgcctcg atcatcggca ggccgatccg gtccttgacc
     3781 gaaccacccg ggttgagcat ctcgagcttg gcgaggatca gcggctgacg atcgatcggg
     3841 ccgaggtcgc gggtcacccg gctcagccgg acgagcggcg tgtgaccgac gaggtcgagg
     3901 atcgtttcgg cgtagtgcat ggaccggagg atagccgggc cgtcacgccg gcggctcgag
     3961 cgcgacggac ggagcgaggg ccgggtcgca gggcccgtgg tcgagtggcg tccgggatca
     4021 ggacacggtg acgtcggagg cgaggcggtc gtcgacgagg acggccgcga tcgtccgact
     4081 ccgcgcctgc ccgccaaggg cctcgacgaa cggtccgcga tcgatcccga gcaggacgac
     4141 gtcgtcgagc gccgtgaccg tcgccgttcg cgggacgtcg cggaccaggg cgatctcgcc
     4201 gaacccgtcg ccgggtccca accgccgggc ctcgcggccc atgacgctga cgcccagcgc
     4261 gcccgtttcg acgacataga agcgatcccc ggggtcgcct tcccggatca ccgtcgtgcc
     4321 ccgcgagtga tggctgcgga cgatgccgcc ggcaagtcgt tcgaggaccg ggcccgagag
     4381 cggcgccagc atcgggaccc cgcgcagcag gcggagctcc gcggcgcgcc ccacatcgag
     4441 tcgatcgccg cggacgatgg cgctcccgac gaggaccagg acgaccggca gcgagagacc
     4501 ggcgaccgcg aacgcggcgc gattgccgac gagggtgatc gccagcggga ccgcgagggc
     4561 accgagcgcc gtcgcgccca tcatcatgcc ctggagcacc ccgaagacgc ggctcatcac
     4621 gtcgtcgccg gccagccggt ggatgagtgt ctgggcgccg acgttggtga gaccggagcc
     4681 gatcccggcg aggaccagga gcgccgcggc cgcgaaggat cccgtcgcga tcgcgatcgt
     4741 ggcgatcccc gctccgaaca ggacggcgct ggccgccagc gacaacccga gccgctcgcg
     4801 cccgacgagc aggagcccgg ccacggaacc gaggatcgcc ccgacaccgc cgatcgcgcc
     4861 gacgaagccg accccgccgc cgtcgaggcc catgagctcg atcgcgagga ccgcatagag
     4921 gatgtccatc gctccgacga ggaaggtccc ccaggtcgcg atcaggacga ccgcccggag
     4981 ccggtcgtcg gcgaggatcg ccgagacgcc gccgatcagc tcgctggcgg tcgaccgcca
     5041 gcggtcgatt cgcggggctg cgtcgacggc gtcaggaact gagcccccga ccggggcgaa
     5101 ggccgcggcg tctccgcggc ggaggaacga ggaccgtcgg acgaccccca ggacggccag
     5161 gctgccgacg acgttgccga cggccgccac gacgaacacg gccgccgggc cgccgagacc
     5221 gagcacgaac ccggccccca gcggaccgat cagcgagccg gcgctctcgc ccatccccga
     5281 cacgacgttg gccgcggtca ggtcgtcggg tgacgtgacg acctccggca tgagcgcgga
     5341 gtggatcggc cgggacaacg ccacgagggt tgccgtgatc gtcgcgagca ggtagatcag
     5401 cagagggtgg gatccgg
//
LOCUS       DATAIW010000159         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_182471, whole genome shotgun sequence.
ACCESSION   DATAIW010000159 DATAIW010000000
VERSION     DATAIW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182471"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VHM48_05085"
     CDS             complement(<1..312)
                     /locus_tag="VHM48_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2754813.1"
                     /translation="MKVLFIGGTGLISSACTRLAVERGVELFLLNRGSNPDRARGAMS
                     LVADLRDEAATKRALAGHRFDVVVDWIAFTPDDIERDLRLFRDRADQFVFISSASAYL
                     KP"
     gene            complement(462..1895)
                     /locus_tag="VHM48_05090"
     CDS             complement(462..1895)
                     /locus_tag="VHM48_05090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754814.1"
                     /translation="MSRRRLPRVAGLRWRGALRHRNFRLFWFGQLISLVGTWMQSVAQ
                     AWLVLQLTHDPFWLGVVAAIQFTPVLVLGLFGGILADILPKRRTIIGTQAILMVLALV
                     LAGLSWSGIVQVWHVLALAFALGLVNAVDMPTRQAFVMEMVGREDVANAVGLNSAMFN
                     AARILGPAVAGLVIGVAGVTICFFLNGLSFVAVIASLLAMREADLRGGARLARPHSVG
                     EVGANLGEGLRYVRRTPVVLLAVTLVGLVATTGMNFNVLMPAMADGVLHVGASGFGFL
                     MSAMGLGSLMAALAIAYLRRPKLSILLGGAFALGALEVVFAGVRFFPLALVAMFGAGA
                     GAIAMTATANSAIQLAVPDALRGRVLSVYTTVFAGSTPVGGLFAGWLASSFGTPVALA
                     VGGGTSALVAVGATVWVMRSGHFAVPRLGRPRPASRGEVGFQNERLAHGVGRHVLDVG
                     GGESSCRPQSPAPRGRPGADSDLQSRG"
     gene            complement(1892..2392)
                     /locus_tag="VHM48_05095"
     CDS             complement(1892..2392)
                     /locus_tag="VHM48_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2754815.1"
                     /translation="MTTALPRTQASASDRLLRIQEVAEETGLTPRSIRYYEERGLLRP
                     AARSEGAYRLYDASDLEQLRLIKGLRDDAGLSIADIGQILEDEESRRRTRAALKATDD
                     PVERRRILLERLASIEGLHGRLNAKLARLQAMFDDVEARRGRLHDLLAAAEQELAAAT
                     EPGSDR"
     gene            complement(2542..>3365)
                     /locus_tag="VHM48_05100"
     CDS             complement(2542..>3365)
                     /locus_tag="VHM48_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2754816.1"
                     /translation="AGGNVGAVVGAAVGALIIASFGWRGAAIVFGIPAIAISIAILLL
                     VRESGTDRAAAVAGGSVRAAFRTIVHDRDLRWLYLTSILGGGGRGLGVVNLFALIYLT
                     KVIGLSDSLSGLMYGALIVFSVPMPLVAGWLSDRIGRKPLIVGVYLGGAIGFVVFLAS
                     GSSVVGLWAGIVLMGLFSFAESPQLQALLADIARPSIRDASFAVYFTLAFGVGSLWVA
                     IYGAIIGAAGNSAGLPIVFSLMAVAFMLAAVGTLPIRAEQRAHENAAYEASLGGQ"
BASE COUNT          578 a         1190 c         1188 g          409 t
ORIGIN      
        1 cggcttcagg taggcgctgg ccgagctgat gaagacgaac tggtcggccc ggtcgcgaaa
       61 gaggcgaaga tcacgctcga tgtcgtcggg tgtgaaggcg atccagtcca ccacgacatc
      121 gaaccggtgg ccggccagcg cacgcttcgt ggccgcctcg tcccgcaggt cagcgacgag
      181 cgacattgcg ccgcgcgccc ggtccggatt gctgccgcga ttgaggagga agagctccac
      241 gccgcgctcc acggccaggc gggtgcaggc gctgctgatc aggccggtgc caccgatgaa
      301 caggaccttc atcgctcgcc tccctcggga ccggccagcg agcgcagggt ttcggggatc
      361 tcgcgagcgc cggcacccac tattgtccct gaagccgggt ggcgcgtcgt tggctggtct
      421 tcctgcgagg tgcccggcgc cgtcggccag cggcgcgtgg atcaccctcg ggattgaagg
      481 tcggaatctg cgcctggccg tccgcgcgga gccgggctct gcggacggca cgacgactca
      541 ccgccgccca cgtcgagtac atggcgaccg actccgtgag cgaggcgctc gttctggaag
      601 ccaacctcgc cacgcgatgc cggccgcggc ctaccgaggc gcggcaccgc gaagtggccg
      661 ctccgcatga cccacacggt ggccccgacg gcaaccagcg ccgacgtccc gccgcccacc
      721 gccagcgcga ccggcgtccc gaaggacgag gccaaccacc cggcgaacag gccgcccacc
      781 ggggtcgagc ccgcgaacac cgtcgtgtag acgctgagga cgcggccgcg cagcgcatcc
      841 ggtaccgcca gctggatcgc cgagttcgcg gtggccgtca tggcgatcgc gcccgccccc
      901 gccccgaaca tcgccaccag ggccagcggg aagaagcgga ccccggcgaa caccacctcc
      961 agggctccga gggcgaacgc cccgcccagc aggatcgaga gtttcggccg gcgcagatag
     1021 gcgatcgcca gggcggccat cagcgagccg aggcccatcg ccgacatgag gaacccgaag
     1081 cccgacgcgc cgacgtgcag cacgccgtcg gccatggccg gcatcaggac gttgaagttc
     1141 atgcccgtgg tcgccaccag gccgaccagg gtcaccgcca gcaggaccac cggcgtccgg
     1201 cggacgtatc ggaggccctc gcccaggttc gcgcccacct cgcccacgct gtgggggcgg
     1261 gccaggcgcg ccccgccgcg caggtccgcc tcgcgcatcg ccaggaggct ggcgatgacc
     1321 gccacgaagc tcaggccgtt gaggaagaag cagatcgtca cgcccgccac gccgatgacg
     1381 aggccggcca cggccgggcc gaggatgcgg gcggcgttga acatggccga gttcaggccg
     1441 acggcattgg ccacgtcctc gcgcccgacc atctccatga cgaaggcctg gcgagtcggc
     1501 atgtcgacgg cgttcacgag gccaagggcg aaggcgaggg cgaggacgtg ccagacctgg
     1561 acgatgccgc tccaggacag gccggccagg accagggcca ggaccatgag gatggcctgg
     1621 gtaccgatga tcgtccgccg cttggggagg atgtcggcga ggatgccgcc gaagagtccg
     1681 agcacgagca ccggcgtgaa ctggatcgcg gcgacgacac ccaaccagaa cggatcgtgg
     1741 gtcagctgca gcacgagcca ggcctgggcg accgactgca tccaggtccc gacgagcgag
     1801 atcagctggc cgaaccagaa gagccggaag ttgcggtggc gcagggcgcc gcgccagcgg
     1861 agccctgcga cccggggcag ccggcggcgg ctcaacggtc cgaccccggt tcggtcgcgg
     1921 cagccagctc ctgttcggca gcggccagga ggtcgtgcag cctgcctcgc cgggcctcga
     1981 cgtcatcgaa catcgcctgg aggcgtgcca gcttggcgtt caggcgcccg tgcaatcctt
     2041 cgatgctggc caggcgctcg agcaggatcc gccgccgctc gactgggtcg tcggtcgcct
     2101 tcagcgccgc gcgggtccgc cgccgcgatt cttcgtcctc aaggatctgg ccgatgtcgg
     2161 ctatggacag gccggcgtcg tcgcgaagcc ccttgatgag gcgaagctgc tccaggtccg
     2221 aggcgtcgta gaggcgatag gctccttcgg accgtgccgc cggccgcagg aggccgcgct
     2281 cctcgtagta gcgaatcgag cgcggagtga ggccggtctc ctcggccacc tcctggatgc
     2341 ggaggaggcg atcggaggcg ctggcctggg ttcggggcaa ggctgtcgtc acctgggtga
     2401 tgataccaaa ccttaccgtt tacgtaaagg ttatggttgg ctggcggtca tgcccgagcc
     2461 aaacaggcga ggagccccgt ccctttccgg ccccggcaca cgagggtcgg gcagtcggca
     2521 cgaggccgac gtgtcagagc gctactgccc tccgagcgac gcctcgtacg cggcgttctc
     2581 atgcgctcgc tgctcggccc ggatcggcag agtccccacg gccgccagca tgaacgccac
     2641 cgccatcaag ctgaacacga tcggcagccc ggccgagttg ccggcggcgc cgatgatcgc
     2701 gccgtagatc gcgacccaca gcgacccgac cccgaaggcg agggtgaagt agaccgcgaa
     2761 cgaggcgtcc cggatcgacg gccgggcgat gtcggcgagg agcgcctgga gctgcgggct
     2821 ctccgcgaag ctgaacaggc ccatgaggac gatcccggcc cacagcccga caacgctgga
     2881 ccccgaggcg aggaagacga cgaatccgat cgcgccgccg aggtagacgc cgacgatgag
     2941 cggcttccga ccgatccggt cggacagcca gcccgcgacc agcggcatcg gcaccgagaa
     3001 gacgatgagc gcgccgtaca tcagccccga cagggaatcg ctcaggccga tcaccttcgt
     3061 gaggtagatg agcgcgaaga gattgacgac ccccaacccg cgcccgccgc cgccgaggat
     3121 cgaggtcagg tagagccagc gcaggtcgcg atcgtggacg atcgtccgga acgcggcccg
     3181 cacgctcccg ccggcgactg cggcggcccg gtcggtgccg ctctcgcgga cgaggaggag
     3241 gatcgcgatg gagatcgcga tcgccgggat cccgaagacg atggccgcgc cgcgccagcc
     3301 gaacgaggcg atgatcagcg ccccgaccgc cgccccgacg accgccccga catttccgcc
     3361 ggcga
//
LOCUS       DATAIW010000160         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_97626, whole genome shotgun sequence.
ACCESSION   DATAIW010000160 DATAIW010000000
VERSION     DATAIW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97626"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1890)
                     /locus_tag="VHM48_05105"
     CDS             complement(301..1890)
                     /locus_tag="VHM48_05105"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEX2754817.1"
                     /translation="MTTDLGPLDRLADRAETLAGAWAGRARASTTVGRERAMLRMFGV
                     SGLDAAGRPLAWSVVDRYLGGGPGRLGGGIVLPFAIALAEYDLDPQRLALDVASGAVD
                     LGLEGALLREPDRRAIAEEEASRLVAAAVERIDANRTARSELIALLGEPGRPWIAAAI
                     SGLDLGEALDEARIAVEAGADLLQIEVPIGRELTDHLRRAGRDVPDWRPRAGIASPDP
                     DLLELAPSGSQRALGSLRRDLDEAAAERRGYVRLGIVPPPLGIPESAIVAGFERIDMA
                     VSDPLAEIIDGHVAPDRALADHAFGQALLARAGVVAVLGAGPLVVGPDIARGMPSSPA
                     SRAGRALALQLVAVAIARGNGLPDDQIVVGGLTSWLAGETDPVAFATAEIALRRALLP
                     SIGLVLEEPRAEGRATGDLWPALAAAVQPHQGTAAILRRVPSAADIRSTRIAASVAAA
                     LAASTESRVLVGVALEHARATAAAALQTLDRLADDGWRAVIGDEPPDAADLRLGADAV
                     AERSDPFDPFGEELSRVGLLG"
     gene            complement(2062..2850)
                     /locus_tag="VHM48_05110"
     CDS             complement(2062..2850)
                     /locus_tag="VHM48_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HEX2754818.1"
                     /translation="MQIRVRLFAIQRELAGTREVPLELGDSATIEDAWAAVVARFPVL
                     APGRASVRFARNGDYAEPATALGDGDEVAFIPPVSGGSNESERAATSDASGDTAGPRR
                     ILELRDAPFAPTILSELEARLATDEDGAVVGFVGRTRVSPGTPAPGQEAEAARHAGRL
                     VESLGYEAHDTMAIRILTAIADEIAERFGVERLAIVHRTGEVPLGEASIVVVAAAPHR
                     DAAFRAARYAIDETKARAPIWKAERFADGHVWIGAPARTRPDET"
     gene            complement(2884..3540)
                     /locus_tag="VHM48_05115"
     CDS             complement(2884..3540)
                     /locus_tag="VHM48_05115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX2754819.1"
                     /translation="MKDNRNWVPGAREMRWIEGLTMPIHAALEAIEIAAAPGNIPIVD
                     RDSGRVLAALAAGRRRIVEVGTAYGYSTLWMALGQPADGTIVTIDPDRERTERARGWW
                     REAGIADERITVVTAKALDAFAAGDPALAGPFDLAFIDALKPEYQAYLDALVPRLSPG
                     ALVVADNVLWSGRVTDAALTDPQTTALRDFDAVVLGDPRFNATILPVGDGLLLAGLRG
                     "
     gene            complement(3587..4423)
                     /locus_tag="VHM48_05120"
     CDS             complement(3587..4423)
                     /locus_tag="VHM48_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754820.1"
                     /translation="MPAERIAENLAFINWTVLTGLAFGSFAAVVLARLRTDATRGFVS
                     FTAACAVAIGILAFLSDQALPTAVAGQPIRIDPVFDLPRRLALGLFCLLAALYVVALI
                     QRRRAPLLAGAAMAAAGASLVFGALGWSSSVLLAIPLLLQFVVLTMATGGVFAAMILG
                     HWYLVTPKLPEAPLILISRILLAVVGLQVVLFAVWVTTGAGPGGGGPFAVLVGPWAMF
                     VWLRLIVGLIFPLIVSWAAVQTARSRSMESATGLLYINVGTIAAGTILAAGLYFGAGL
                     LV"
     gene            4818..>5086
                     /locus_tag="VHM48_05125"
     CDS             4818..>5086
                     /locus_tag="VHM48_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2754821.1"
                     /translation="MAINPNTTFTWFGHSCWEVTTPGGKTILLDPWFGNPLSPRPPGA
                     VDRCDVLLVTHGHDDHFHDAVSIASRTRPVWPCIHEMSLWLGRNYA"
BASE COUNT          767 a         1849 c         1842 g          628 t
ORIGIN      
        1 aggcctgcgt cccatcgacg aacacgttga tgtgctcgcg cagcgtcggt ccggaccggc
       61 tcgacgatcc acgtgattcc ggcggtctca ggcggctgag gggcgcggcc gggcgttgtc
      121 gcgtggccgg aacgagacgg ccagcggccc gtgcgaatcg ccgaatacct cgcgacatcc
      181 ggcgggatcg cgtccgcccg atgctccggc gcaccatccg aagatgcggc tattcgaaac
      241 ggggggcggg ccctcccgcg gcgggacggc cgatctcgcg cgcgaacgcg aggacggagc
      301 tcagcccagc aggccgaccc ggctcagctc ctccccgaac ggatcgaacg gatcggagcg
      361 ttcggcgacg gcgtcggcgc ccaggcgaag gtcggccgcg tcgggtggct cgtcgccgat
      421 caccgcccgc cagccgtcgt cggcgagccg atcgagcgtc tgcagcgcgg cagcggcggt
      481 ggcgcgtgcg tgctcgaggg cgacgccgac cagcacccgc gactcggtcg atgccgcgag
      541 agcggcggcc accgacgcgg cgatccgggt cgaccggatg tcggcggccg acggaacgcg
      601 ccgcaggatc gcggccgtgc cctggtgcgg ctggacggcg gcggcgagcg ccggccacag
      661 gtcaccggta gcccgcccct ccgcgcgcgg ttcctcgagg acgagtccga tcgacggcag
      721 aagggcgcga cgaagtgcga tctcggcggt cgcgaacgcc accggatccg tttcgccggc
      781 gagccacgag gtcagtccgc cgacgacgat ctggtcgtcc ggcaggccgt tgccgcgagc
      841 gatcgcgacg gccacgagct ggagcgcaag ggcccgtccg gcacgcgacg ccgggctcga
      901 aggcatgccc cgggcgatgt cgggcccgac gacgagcgga ccggcgccga gcaccgccac
      961 gacccccgcc cgggcaagga gcgcctgccc gaacgcatgg tcggcgagtg cccgatcggg
     1021 ggcgacgtgc ccgtcgatga tttccgccag tggatccgag acggccatgt cgatccgctc
     1081 gaagccggcc acgatcgcgc tctccgggat cccgagcggc ggaggcacga tcccgagccg
     1141 gacatagcca cggcgttcgg cggcggcctc gtcgaggtcg cggcggaggc tgccgagggc
     1201 gcgctgactg ccgctcgggg ccagttcgag gaggtccgga tcgggggacg cgatgccggc
     1261 tcgcggccgc cagtccggca catcgcgccc ggcccgccgc aggtgatcgg tcagctcgcg
     1321 cccgatcggg acctcgatct ggagcaggtc ggcgccggcc tcgaccgcga tccgggcctc
     1381 gtcgagcgct tcgcccaggt cgagcccgct gatcgccgcc gcgatccacg gccggcctgg
     1441 ctcgccgagc agggcgatga gctccgatcg cgccgtccgg ttggcatcga tccgctcgac
     1501 cgcggcggcg acgaggcgac tggcctcctc ctcggcgatg gcccgccgat ccggctcgcg
     1561 caggagggcg ccctccagcc ccaggtcgac cgccccggac gcgacgtcca acgccagccg
     1621 ctgcggatcg aggtcgtact cggcgagggc gatcgcgaac ggcaggacga tcccgccacc
     1681 gagccggccg gggccgccac cgagatagcg gtcgacgacc gaccaggcga gcggccgccc
     1741 ggccgcatcg agcccactga ccccgaacat ccggagcatg gcccgctcgc gcccgaccgt
     1801 ggtcgacgcg cgggcccgac cggcccaggc gccggcgagc gtctccgcgc ggtcggcgag
     1861 gcggtcgagc ggtccgaggt cggtcgtcat cgaccgcagt atgcggtcag cgcggtctgc
     1921 gctgggcgat cagcccgagg agcaggtccg gaaacgcccg gatgtcgagc ggcttgcccc
     1981 gaatgcgtcg catccggtgt cgaggcagcc gccggtaaac ggggccacgt ggccacgtgg
     2041 ccacgccgcg gcggaccccg atcaggtctc gtcgggtcga gtccgggcgg gcgcaccgat
     2101 ccagacgtgg ccgtccgcga agcgctccgc tttccagatc ggcgcccgcg ccttggtctc
     2161 gtcgatggca tagcgagccg cgcgaaacgc cgcgtcgcgg tgcggcgccg cggcgacgac
     2221 gacgatgctc gcctcgccga gcgggacctc gcccgtgcgg tggacgatgg cgaggcgctc
     2281 gacgccgaat cgttcggcga tctcgtcggc gatggccgtc aggatccgga tcgccatcgt
     2341 gtcgtgggcc tcgtagccga gcgactccac caggcgcccg gcgtgacgag ccgcttccgc
     2401 ctcctggcct ggtgcgggcg tcccggggga gacacgcgtc cgcccgacga acccgacgac
     2461 cgcgccgtcc tcgtcggtcg ccaggcgggc ctcgagctcg gacaggatcg tcggcgcgaa
     2521 cggtgcgtct cgaagctcga ggatccgccg cggaccggcg gtgtcgccag aggcgtcgct
     2581 cgtggcggcg cgctcagact cgttggatcc accgctgacc ggcgggatga acgccacctc
     2641 gtcgccgtcg ccgagggccg tcgccggctc cgcgtagtcc ccgttccggg cgaaccggac
     2701 cgaggcgcga cccggggcga ggaccgggaa ccgggcgacg accgccgccc aggcgtcctc
     2761 gatcgtggcg ctgtcgccca gctcgagggg cacctcgcgg gtacccgcga gctcccgctg
     2821 gatcgcgaac agtcggacgc ggatctgcac gtgggcgctc ctcagccggt caacccgtcc
     2881 cggtcaaccg cggaggccgg cgaggagcag gccgtcgccg accggcagga tcgtcgcgtt
     2941 gaaccgcgga tcgccgagaa cgaccgcatc gaagtccctg agggcggtcg tctgcggatc
     3001 ggtgagggcg gcgtcggtca cccggccgct ccacaggacg ttgtcggcga cgacgagcgc
     3061 gcccggcgag aggcgtggga cgagggcgtc gagataggcc tggtactcgg gcttgagcgc
     3121 gtcgatgaag gcgaggtcga atggcccggc cagggccgga tcgcccgcag cgaatgcgtc
     3181 gagcgccttg gccgtgacga ccgtgatccg ctcgtccgcg atgcctgcct cgcgccacca
     3241 gccgcgggcg cgctcggtcc gctcgcgatc cgggtcgatc gtgacgatcg tcccgtcggc
     3301 cggctgaccg agcgccatcc agagcgtcga gtagccgtac gccgtcccga cctcgacgat
     3361 ccggcggcgt ccggcggcga gcgcggcgag cacccggccg ctgtcccgat cgacgatcgg
     3421 gatgttgccc ggggccgcgg cgatctcgat cgcctcgagc gccgcgtgga tcggcatcgt
     3481 caggccctcg atccagcgca tctcgcgcgc gcccgggacc cagttccgat tgtctttcat
     3541 cgtccctccc gttccggccg cccgatgtcg atcgatccgg tggtcgtcat acgagcagtc
     3601 ccgccccgaa gtacaggccg gccgcgagga tcgttccggc ggcaatcgtg ccgacattga
     3661 tgtagagcag cccggtcgcc gattccatgg accggctgcg ggcagtctgg acggccgccc
     3721 acgaaacgat gagcgggaag atcagcccga cgatcaggcg gagccagacg aacatcgccc
     3781 acggtccgac gaggacggcg aacggcccgc cgccacccgg ccccgccccg gtcgtcaccc
     3841 agaccgcgaa caggacgacc tgcagaccga ccaccgcgag caggattcgc gagatcagga
     3901 tcaggggcgc ctcgggcagc ttcggtgtga ccaggtacca gtgaccgagg atcatcgccg
     3961 cgaacacgcc gccggtcgcc atcgtgagga cgacgaattg gagcagcagc gggatcgcga
     4021 gcaacacgct gctgctccag ccgagcgcac cgaagacgag cgaggcgccg gcggcggcca
     4081 tcgctgctcc ggcgaggagc ggcgcgcgac ggcgctggat gagcgccacg acatagagcg
     4141 cggcgaggag gcagaagagc ccgagggcca gtcggcgagg caggtcgaag acaggatcga
     4201 tccggatcgg ctggccggcg acggctgtcg gcagggcctg gtcggacagg aacgccagga
     4261 tgccgatcgc caccgcgcag gcggcggtga acgacacgaa cccgcgggtc gcgtcggtcc
     4321 gcagccgggc gaggacgacc gccgcgaacg agccgaaggc cagtcccgtc aggaccgtcc
     4381 agttgatgaa cgcgaggttc tcggcgatcc gctcggcagg cacgatccga gtgtagccga
     4441 ggcaccctgg tttcccggcc ggccggccgc cctgacgaag cagccgcccg gagtgtcgtg
     4501 cagacagctc gtaccgatct gccatccacc atccccggca gtgagagtca aggtcgaccg
     4561 gctcgcgaca ctcctgagca ggcggaatcg gtcgaaatcg cgtgtcactt cggtcatcca
     4621 ccatcccggg cggtgggtgg caccaccgaa gcccgcgacc cgtgacgcgc aacccccggt
     4681 ggtgaatggc acgccccact tgctctgcgc cgggtcgggc cgccgatcgc ggaccgctcg
     4741 gccctgcaat ccggcgccga acggacgaag atccttgcaa cagccttccg cccagctcac
     4801 gagaggatcg acgaccgatg gccatcaacc ccaacacgac gtttacctgg tttggtcact
     4861 cctgctggga ggtgacgact cccggcggca agacgatcct cctcgaccca tggttcggca
     4921 atccgctcag cccgcgccca ccgggcgccg tcgaccgctg cgacgttctg ctcgtcaccc
     4981 acggccacga cgatcacttc cacgacgccg tgtcgatcgc cagccggacg cgcccggtct
     5041 ggccgtgcat ccacgagatg agcctctggc tcggccggaa ctacgc
//
LOCUS       DATAIW010000161         5804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_7904, whole genome shotgun sequence.
ACCESSION   DATAIW010000161 DATAIW010000000
VERSION     DATAIW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7904"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VHM48_05130"
     CDS             <1..426
                     /locus_tag="VHM48_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX2754822.1"
                     /translation="QPGDLVIWGGGTHVGIYLGNGMAISTLLNGVRIHSIRATTARFT
                     AFLHTGIAGMAATAVPRPAQATAVVAKAATVTARRVTTTALRLRSGPSTAARVLSTLA
                     AHARVGVVRVAKDRSGRTWYGVLVGGRLGWLAGWYTRAI"
     gene            complement(653..1678)
                     /gene="mgrA"
                     /locus_tag="VHM48_05135"
     CDS             complement(653..1678)
                     /gene="mgrA"
                     /locus_tag="VHM48_05135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX2754823.1"
                     /translation="MTYLPAPTRYDDMLYRRSGRSGLHLPAMSLGLWQNFGHDRSIDS
                     QRAVIRRAFDLGITHFDLANNYGPPYGAAEENFGRVLAADLSGHRDELVISSKAGYDM
                     WPGPYGNWGSRKYLTASLDQSLRRLGLEYVDIFYSHRFDPETPLEETMVALDTAVRQG
                     KALYVGISSYSASQTREAVAILRELGTPLLIAQPSYSMLNRWIEGGLLDVLGDEGVGC
                     IVFSPLAQGLLTGKYLDGIPAGSRGAAGGSLPPTLLGDDTLAKVRALADLAKRRGQSL
                     AQMALAWTLRDPRVTSALVGASSVGQLEENVAALQHLEFSATELADIDRYATESGINL
                     WARSSEA"
     gene            complement(2332..3735)
                     /locus_tag="VHM48_05140"
     CDS             complement(2332..3735)
                     /locus_tag="VHM48_05140"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX2754824.1"
                     /translation="MTTETTGRGSAGGNSSTSEPATTAERVLDLVAEQAGPAEAEVTV
                     RRGIDSLTRFATNFIHQNVAEEVSHVLLRVALDGRVASGALDGPVDDGTLRRLVADVV
                     DAARIAPADPDWPGLTVPTPASDADAPPVGHWDEATAAGRPDERARRVRDFVDAAGGL
                     ETAGFCSTSAITVAFANSAGQWLTGRGTTANLDGIARTPTADGSARRVSVRLADLAGR
                     PVGERAAGKARSASDPTDLSPGRYEVILEPECVANILSFLLVHGFNGRAVEDGRSFVR
                     LGETQFDPSITLRDDVADPDMVGIPFDIEGTPKRRRDLVSAGVTSGLLHNRRTARKAG
                     AESTGHAVEGGGTWGALGANLVMTAGAKTADELIAGVDRGVLVTDFWYTRILDPKTQV
                     VTGLTRNGVWLIERGRIVRPVTNLRFTQSFIEAFAPGTVRAVGAERALLFGGWDSVYL
                     VPTLHLASWNFTGGARG"
     gene            complement(3732..5174)
                     /locus_tag="VHM48_05145"
     CDS             complement(3732..5174)
                     /locus_tag="VHM48_05145"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2754825.1"
                     /translation="MYDVATAAVEAALNAGARYADARVMEMRTEAMAARNGAVESLDR
                     EERAGVGVRALIGSSWGFFAVPDVGSAAARRAGEQAAAVARASTLVPGADLGLVEEPA
                     TQGSWQNDYRADPWSVSLAEKGDLLESVTRTMGEHGADVSEASHRIWDTRKWLVSSEG
                     HRIDQHIVECGASISALAVGERETQQRSYPGIRGQYGTRGWELIRELDLPANAARIAE
                     EARALLTADQCPSLDATDLILGSEQMALQIHESVGHAVELDRILGWEAAFAGTSWLEL
                     PKLGSLRFGSELMNITADATLPGALGSFGFDDEGTPAHPVDIVRDGVWVGTLSGRDSA
                     ALAGIERSAGAIRAEGFNRIPMVRMTNVGLLPGSDSLDAMIAATDDGILMDTNRSWSI
                     DDKRLNFQFGCQIGWEIKGGKRGRLLKNPTYTAISPRFWGSLDMLGGPDEWTFWGTPN
                     CGKGQPQQIGHTGHPAVPARFRGIRVGVRG"
     gene            5399..5665
                     /locus_tag="VHM48_05150"
     CDS             5399..5665
                     /locus_tag="VHM48_05150"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX2754826.1"
                     /translation="MQETHDGSHRDGLCSFEVLIEDYTLGGDPALALMARVVHGADVS
                     EDRDATPQSPGLLAIDDQRQLELELPVYDALYAWAQREVASRAS"
BASE COUNT          864 a         2067 c         2052 g          821 t
ORIGIN      
        1 cagcccggcg acctcgtgat ctggggcggc gggacccacg tggggatcta ccttgggaac
       61 ggcatggcga tctcgacgct cctgaacggg gtccgcatcc actcgatccg ggccaccacc
      121 gcgcggttca cggcgtttct ccataccggc atcgccggta tggcggcgac cgcggtcccg
      181 cgcccggcgc aggcaacggc ggtggtcgcg aaggccgcga ccgtcacagc ccgccgcgtc
      241 acgacgaccg cgctccgcct ccggtccggc ccctccacgg cagcccgcgt gctctccacg
      301 ctggccgcac acgcgagggt gggcgtggtc cgcgtcgcga aggaccgctc cggtcggacc
      361 tggtacggcg tcctcgtcgg gggccgcctc ggctggctgg ccggctggta cacgcgcgcg
      421 atctgaccgt ttcgccaccc cgccgtcccg cgagcccgcc ccggatcccc ggcggcgggc
      481 tcgcccgtta agggcctcgc aggggagatg gcggccccgg acaccgggtg ccggtgccgg
      541 ctgccgggcc ccagtgcggg cgtcggcgcg gatgttggca gggcagccag acaagccgcg
      601 cggtcgctgt cagcctggct gtcagtccgc cagcgcccgg cggcggatca cctcacgctt
      661 cgctggatcg cgcccagagg ttgatcccgc tctccgtcgc gtagcgatcg atgtcggcga
      721 gctccgtcgc agagaactcg aggtgctgga gggcggcaac gttctcttcg agttgcccca
      781 cgctggacgc cccgacgagc gccgaggtca cacgcgggtc gcggagtgtc caggcgaggg
      841 ccatctgcgc caggctctga cctcgacgct tcgcgagatc ggcgagcgcc cggaccttgg
      901 ccagggtgtc gtcgccgagg agcgtcggcg ggagggagcc acccgccgcg ccacgcgagc
      961 cggcggggat gccgtcgagg tacttgccgg taagcagccc ttgagccaac ggcgagaaga
     1021 cgatgcagcc gactccttcg tcgccgagga catcgagcag gcccccttcg atccagcgat
     1081 tgagcatgga gtaggacggc tgcgcgatga gcagcggcgt tccgagctca cgcaggatgg
     1141 ccacggcctc gcgcgtctgc gatgccgagt acgacgagat tccaacgtag agagccttgc
     1201 cctgccgcac cgcagtgtcg agggcgacca tcgtttcctc gagcggcgtc tccgggtcga
     1261 accggtggga gtagaagata tcgacgtact cgaggcccaa ccgacggagg ctctggtcga
     1321 ggctggcggt caggtatttg cgcgagcccc agttgccata gggtcccggc cacatgtcgt
     1381 agccggcttt cgacgagatg accagctcat cccgatggcc ggacaagtcc gccgcgagaa
     1441 ctcgaccgaa gttctcctcc gcggcgccgt acggcggccc atagttgttg gccaggtcga
     1501 aatgggtgat ccccaggtcg aaggcccggc gaatcaccgc tctctggctg tcgatcgacc
     1561 gatcgtgtcc gaagttctgc cacagcccga gcgacatcgc gggcaggtga agcccgctcc
     1621 gcccggaacg ccggtacagc atgtcgtcgt agcgggtggg agcgggaagg tacgtcatga
     1681 gttccattct gccactcccg tcccgcaccg ggtgacggcg ggcgcgagcg cccgccggct
     1741 tgcgtctgta tgtcgagcgc cccgtgttgg atcgcgtcgt tgcgggcctc gttgggcaaa
     1801 gagcagcggc cgccggtgtc gcgactgcgc gagcacagta ccgagcacca cccacgcggg
     1861 cagggcagcg agcgcgtagc ggggcatcga gtcgcactgg gacacgagca acggcaacag
     1921 cgtcccgcaa gaaaagaggg cgagatcgat gtctcgccgt cggacgagga gagcgaggcc
     1981 gaccagtggc atgccgagac cgagtgcgat ctggggcagg ctgacaatcg agcgcgaact
     2041 gagcagaccg aggagccgaa tgtggcttcc tgtccggcga agaaggacgg cgtgccgcgc
     2101 aggtagccag ccgggtcgcc gaggatccag gcgatcgccg accaccaggc gacgagggtg
     2161 agtgggccgg cgacgagcag cacggcctca gccagggtgg ggcggcggcg ctcgcggagc
     2221 ggcccggcca gcgccagcag cgagacggcg gggccgacga gcctggtgct gacgaccggt
     2281 tggcgcgaaa ctcggaccgc cagccttgga gcccaggata gagcacggtg atcagcccct
     2341 ggcgccgccg gtgaagttcc atgacgcgag atggagcgtt ggcacgaggt agacgctgtc
     2401 ccagccaccg aacaggaggg cgcgctcggc cccgacggcg cggaccgtcc ccggagcgaa
     2461 cgcctcgatg aacgactggg tgaaccgcag gttcgtgacc ggccgtacga tccggccccg
     2521 ctcgatgagc cagacgccgt tgcgggtcag ccccgtcacg acctgggtct tcgggtcgag
     2581 gatccgggtg taccagaagt cggtgacaag gacgccgcgg tcgacgccag cgatgagctc
     2641 atcggcggtc ttcgcgcccg cggtcatgac gaggtttgcc ccgagcgcgc cccacgtccc
     2701 gccgccctcg accgcgtgac cggtgctctc cgcgccggcc ttccgggcgg tccgccgatt
     2761 gtggagcagg ccgctcgtca cccccgccga gacgaggtcg cgccgccgct tcggcgtccc
     2821 ctcgatgtcg aacgggatgc cgaccatgtc cgggtcggcg acgtcgtcgc ggagggtgat
     2881 cgaggggtcg aactgcgttt ccccgaggcg aacgaaggag cgcccatcct ccacggcccg
     2941 gccgttgaag ccgtgaacga gcaggaagga caggatattg gcgacacact ccggctcgag
     3001 gatcacctcg taccggccag gcgacaggtc ggtcgggtcg ctcgccgaac gggccttgcc
     3061 ggcggcccgc tcgccgaccg ggcggccggc gagatcggcc agcctgacgg aaacccgccg
     3121 ggcgctgcca tcggctgtcg gcgtccgggc gatgccgtcg aggttcgccg tggtcccgcg
     3181 gccggtcagc cactggccgg cgctgttggc gaaggcgacc gtgatggcgc tcgtcgagca
     3241 gaagccagcg gtctcgagtc cacccgccgc atcgacgaag tcgcggaccc gtcgggcgcg
     3301 ttcgtcgggc ctgccggcgg cggtcgcctc gtcccagtgc ccgactggcg gcgcgtcggc
     3361 gtccgaagcc ggggtgggga cggtcaagcc gggccagtcg gggtcggccg gggcgatcct
     3421 cgccgcatca acgacgtcgg cgaccagccg acggagcgtg ccgtcgtcga ccggcccgtc
     3481 gagcgcgccg gaggcgacgc gaccgtcgag ggcgacccgg agcaggacgt ggctgacctc
     3541 ctcggcgacg ttctgatgga tgaagttggt ggcgaagcgg gtcaggctgt cgatgccgcg
     3601 gcggacggtg acctccgcct cggccggacc ggcctgctcg gcgacgagat cgaggacccg
     3661 ctcggcggtc gtcgcgggct cgctcgtcga cgagttccca ccggccgacc cgcgcccggt
     3721 cgtctcggtc gtcatccccg gacccccacc cggatcccgc ggaagcgggc cgggaccgcc
     3781 ggatgaccgg tgtggccgat ctgctggggt tggcccttgc cgcagttcgg cgtcccccag
     3841 aacgtccact cgtccggccc gccgagcatg tcgagcgagc cccagaaccg cgggctgatc
     3901 gccgtgtacg tcgggttctt gagcaaccgg ccccgcttgc cgcccttgat ctcccagccg
     3961 atctggcagc cgaactggaa gttcagccgc ttgtcgtcga tcgaccagga gcggttcgta
     4021 tccatcagga tcccgtcgtc ggtggccgcg atcatcgcgt cgagcgagtc gctgcccggc
     4081 aacagcccga cgttcgtcat ccggaccatc gggatccggt tgaagccttc cgcccggatc
     4141 gcgccggcgg accgttcgat gccggccagc gccgccgaat cccggccgct cagcgtcccg
     4201 acccagacgc cgtcacggac gatgtcgacc ggatgggccg gcgtcccctc gtcgtcgaag
     4261 ccgaagctgc cgagggcgcc gggcagggtc gcatcggcgg tgatgttcat cagctccgag
     4321 ccgaagcgaa gcgagccgag cttcggcagc tcgagccacg acgtcccggc aaaggccgcc
     4381 tcccagccga ggatccggtc gagctcgacg gcatgaccga ccgactcgtg gatctggagc
     4441 gccatctgct cgctgccgag gatcaggtcg gtcgcatcca gggacgggca ctgatcggcc
     4501 gtcaggaggg cgcgcgcctc ctcggcgatc cgcgccgcat tggccggcag atccagctcg
     4561 cggatgagct cccagccgcg ggtgccgtac tgaccccgga tccccgggta gctgcgctgc
     4621 tgcgtctcgc gctcgccgac ggccagcgcc gagatcgacg ccccgcactc gacgatgtgc
     4681 tggtcgatcc ggtgaccctc gctgctgacg agccacttgc gggtgtccca gatgcgatgg
     4741 ctcgcctcgg acacgtcggc gccgtgctcg cccatcgtcc gcgtcacgct ttcgagcaga
     4801 tcgcccttct cggcgaggga caccgaccag ggatccgcgc ggtagtcgtt ctgccatgaa
     4861 ccttgagtcg ccggctcctc gacaaggccg aggtcggcac ccggcacgag ggtcgacgcc
     4921 cgggcgacgg cggctgcctg ctctccggcc cgtcgcgccg ctgccgatcc cacgtccggc
     4981 accgcgaaga agccccagct cgacccgatc agggcccgga cgcccacacc ggcccgctcc
     5041 tcgcggtcga ggctctcgac ggcgccgttg cgggccgcca tcgcctctgt ccgcatctcc
     5101 atcacccgtg cgtccgcgta gcgggcaccg gcgttgagcg cggcctcgac cgcggcggtg
     5161 gcgacatcgt acatcgccca catgctaggc cgatgtcgac ccccgtgcga atctctggga
     5221 tcagctgtca gacgagccct tcgagccgcc gcacggcggc atccggtccg ggaagagaac
     5281 acgcgacggg cacccgcacg aaacccggcg atctctggca gttcggggac catggcccgc
     5341 cccccgacgg tgcgacgccg accctgctga cgaccgatcc accctactgc gtcccaagtt
     5401 gcaggagaca cacgatgggt cccaccggga cggcctgtgc tcgttcgagg tcctcatcga
     5461 ggactacacg ctcggcggcg acccggccct cgccctgatg gcacgggtcg tccacggggc
     5521 cgacgtgtcc gaggaccgcg acgcaacgcc ccagtcaccg ggtctccttg cgatcgacga
     5581 ccagcgccag ctggagctcg agctcccggt gtatgacgcg ctctacgcct gggcgcaacg
     5641 cgaggtcgcg tcgagggcga gttgacacgg gtggtccgcg cgacctcgag cgccgtcctt
     5701 gcccgcaacg gccgcctgct cttcgcgcga cgcggatggc tcgactcttg ggccacggcg
     5761 ctcaccctgg cccgagacgc cacgatcagc ctgcggctga tgag
//
LOCUS       DATAIW010000162         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_97761, whole genome shotgun sequence.
ACCESSION   DATAIW010000162 DATAIW010000000
VERSION     DATAIW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97761"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VHM48_05155"
     CDS             <1..1539
                     /locus_tag="VHM48_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754827.1"
                     /translation="NTTASATSGGEGASGQPDEAAAIATWERRAADLRDRRDRLAAQQ
                     ADADAARREIEGRRARAEATVALDEERIVRSEGDAVALTEREETIVEERRRLAAELSS
                     AVEREERAGAVLAEVRAADAAARDRLAASERAASQARERLRTADERLRAADRADLEAR
                     LGRDALREQFLVELAGLGELGRRRLEAEAGVIPVAVGPGPIPDREDPDPDAATTDPAG
                     SESDDTVALEAALDVVVARWATQPPAGDVPSPGRLGTLRRRYHELGAANPFAVDEYDA
                     LRQRLESLDTQQRDLRTAIDKTRLLIGELNTMIADQFRTTFAALETAFDARFKQLFGG
                     GFARLSLTDPADLAATGVEIVARPPGKKAQALAMLSGGERALTAVALLFAMLEVRPVP
                     FCVLDEVDAALDEANVGRFSDALRSLADRTQFIVITHNRGTIEAADALYGVTVGDDSV
                     SRVISLRLDEAQAIADRQGADARWSDDQLDARGSADPSADPSAVSSPDSSADAASTAL
                     AEVG"
     gene            1539..2699
                     /gene="ftsY"
                     /locus_tag="VHM48_05160"
     CDS             1539..2699
                     /gene="ftsY"
                     /locus_tag="VHM48_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX2754828.1"
                     /translation="MFWRRKREEPEAEPSTDPLESGNTADRRPPDDDDAIANDGPWEP
                     DPADFEPDAAWGPSGAPPFDPEPAPTAEPIFFDAPEPEPIQPVVEPAGSLDHGLARTR
                     GGFMSRLRGFLVGDDGSGPSWDDVEETLIAGDVGAALAMDIVERARGRRDPAGAEAAV
                     RAELTALLVPREPGWEPRPAVSGGPAILLIVGVNGTGKTTTIGKLASRYMAEGRHVLL
                     AAADTFRAAAIDQLMIWADRAGVQVVSHAPGADPGAVVYDALDAAIARGADLMIADTA
                     GRLHTKSNLMDELTKIRRIVDKRLPGAVPETFFVLDATTGQNGLHQAKAFHESVGLTG
                     IVLTKLDSTAKGGIVFAIEHDLGIPVRFVGVGEQAGDLLPFDPDAFVAALFA"
     gene            complement(2706..3071)
                     /locus_tag="VHM48_05165"
     CDS             complement(2706..3071)
                     /locus_tag="VHM48_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754829.1"
                     /translation="MPGWTIGSLLAHLAFWDRLVIERWTRATADGAVAPPGLSEALTD
                     IINAASQAQWWALPGPVAGAEAIAAAEAVDAYVATLAPVQVTTAQTAGLDRLVDRSRH
                     RAEHLDAIEAALAAAAPSR"
BASE COUNT          443 a         1174 c         1162 g          418 t
ORIGIN      
        1 aacacgaccg cgagcgcgac atccggcggc gagggagcgt cgggtcagcc ggacgaggct
       61 gcggcgatcg cgacctggga gcgccgagcc gcggacctcc gggaccggcg cgatcggctc
      121 gccgcccagc aggccgacgc cgatgccgca cgccgcgaga tcgagggccg acgggcccgg
      181 gccgaggcga ccgttgcgct ggacgaggag cggatcgttc gatccgaagg cgatgccgtc
      241 gccctcaccg agcgcgagga gacgatcgtc gaggaacgcc gacgcctggc ggccgagctg
      301 tcgtccgcgg tcgagcgcga ggagcgcgcc ggggccgtgc tggccgaggt gcgcgccgcc
      361 gatgccgccg cgcgcgaccg cctcgctgcc tccgagcgtg ccgcgtccca ggcacgcgag
      421 cgacttcgca cggccgacga gcgcctgcgg gccgccgacc gggcggatct cgaggcgcgt
      481 ctcgggcggg acgcccttcg cgagcagttc ctcgtcgagc tggccggcct gggcgagctc
      541 gggcgacggc ggctggaggc cgaagccggg gtgattccgg tggcggtcgg gccgggtccg
      601 atccccgatc gagaggaccc ggacccggac gcggccacga ccgatccggc cgggagcgag
      661 tccgacgaca ccgtggccct cgaggcggcg ctcgatgtcg tcgtcgcccg ctgggcgacc
      721 cagccgccgg ccggcgatgt cccgtcgcct ggtcgtctcg gcacgctccg tcgtcgatac
      781 cacgagctgg gggctgcgaa cccgttcgcg gtcgacgagt acgacgccct ccgccagcgc
      841 ctcgagagcc tcgacaccca gcagcgcgac ctgcggacgg cgatcgacaa gacccgcctg
      901 ctcatcggcg agctgaacac gatgatcgcg gaccagttca ggacgacgtt cgcggccctc
      961 gagacggcgt ttgacgcgcg gttcaagcag ctgttcggtg gcggttttgc gcggctgtcc
     1021 ctgaccgacc cagccgacct cgccgcgacc ggggtcgaga tcgtcgcccg accgccgggc
     1081 aagaaggccc aggcgctggc gatgctctcg ggcggcgagc gggccctgac cgcggtggcc
     1141 ctcctgttcg cgatgctcga ggtccggccg gtgccgttct gcgtcctcga cgaggtcgac
     1201 gccgccctcg acgaggcgaa tgtcgggcgg ttctccgatg cgctccggag cctcgccgac
     1261 cggacccagt tcatcgtcat cacccacaac cggggcacga tcgaggccgc cgacgcgctc
     1321 tacggcgtga ccgtcggtga cgattcggtc agccgggtga tcagcctccg tctcgacgaa
     1381 gcccaggcga tcgccgatcg gcagggcgcc gacgcccgat ggagcgacga ccagctcgat
     1441 gcgcgcggat cggccgatcc gtccgccgat ccgtccgccg tttcgtcccc ggattcgtcc
     1501 gccgacgcgg cctcgaccgc tctcgccgag gtcggctgat gttctggcga cgcaagcgcg
     1561 aggagcccga ggccgagccc tcgacggacc cgctcgagtc cggcaacacg gccgaccggc
     1621 ggccgcccga cgacgacgac gcgatcgcca acgacggacc gtgggagccc gatcccgcgg
     1681 acttcgagcc tgacgcggcg tggggcccgt ccggtgctcc gcccttcgat cccgaacccg
     1741 caccgaccgc cgagccgatc ttcttcgacg ctcccgagcc cgaacccatc cagcccgtcg
     1801 tcgagccggc cggctccctc gatcacggcc tggcgcgaac ccgcggcggg ttcatgtccc
     1861 ggctgcgcgg cttcctcgtc ggcgacgatg ggagcggccc gtcctgggac gacgtcgagg
     1921 agacgctgat cgcgggcgac gtcggagcgg ccctggcgat ggacatcgtc gagcgggcgc
     1981 gcggccgccg cgatcccgcg ggggcggagg ccgccgtccg cgccgagctg accgcgctcc
     2041 tcgtgccgcg cgagcccggc tgggaacccc gcccggcggt cagcggcggg ccggcgatcc
     2101 tcctgatcgt cggggtcaac gggacgggca agacgaccac gatcggcaag ctcgcgagcc
     2161 gctacatggc cgaggggcgt cacgtcctgc tggcagccgc cgacaccttc cgggccgcgg
     2221 cgatcgacca gctgatgatc tgggccgacc gagccggggt ccaggtcgtg tcgcacgcgc
     2281 ccggcgccga tccgggtgcc gtcgtctacg acgccctgga cgcagcgatc gcccgcggcg
     2341 ccgatctcat gatcgccgac acggccggcc ggctgcacac gaagtcgaac ctgatggacg
     2401 agctgacgaa gatccgccgg atcgtcgaca agcgcctgcc gggcgccgtc cccgagacgt
     2461 tcttcgtcct cgatgcgacg accggccaga acggactcca ccaggcgaag gcgttccacg
     2521 aatcggtcgg gctcaccggg atcgtcctga ccaagctcga ctcgacggcc aagggcggga
     2581 tcgtcttcgc gatcgagcac gacctcggga tccccgtccg gttcgtcggg gtgggggagc
     2641 aggccggcga tctgctgccg ttcgatcccg atgcgttcgt cgcggcgctg ttcgcctgag
     2701 ccgcgtcagc gagagggcgc ggctgccgcg agagcggcct cgatggcgtc gagatgctcc
     2761 gcgcggtggc gcgaccgatc gacgagccgg tccagtcccg cggtttgagc cgtcgtgacc
     2821 tggaccgggg ccaacgtcgc gacgtacgcg tcgacggctt cggctgccgc gatcgcctcc
     2881 gcgccggcca ccggaccggg aagcgcccac cactgggcct gcgacgccgc gttgatgatg
     2941 tccgtcaatg cctcgctgag gccgggtggc gccactgctc catcggccgt ggcccgggtc
     3001 cagcgctcga tcacgagccg gtcccagaac gcgagatgag cgagcaacga tccgatcgtc
     3061 cagccgggca tcgcctcgag ggtgtgacgc ggcgaggtct cgtcacgggg cgcagactga
     3121 tcgggccgct cattgactcg ctcgctgagt cagattccgg ctcagcgtgc agagctcggt
     3181 cgcgactcgg acccggg
//
LOCUS       DATAIW010000163         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_38334, whole genome shotgun sequence.
ACCESSION   DATAIW010000163 DATAIW010000000
VERSION     DATAIW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38334"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VHM48_05170"
     CDS             complement(<1..1281)
                     /locus_tag="VHM48_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX2754830.1"
                     /translation="MATDVQDVLGASVKRVEDPRFITGKGRYLDDIKLTGMVHMAILR
                     SPYAHANIRSVDTSKAKTMPGVLAVIVGADIPWNPLPMAWPAGGASGITNNISTPRIL
                     ATDSVKWTGEGVAAVVAETAEQAIDALDGIHVDYEPLATVVDAEKATQPGAVQLHDNA
                     PNNVVFDWTVGDKAGTEKALAEAEVVVRERLVNHRLIPNPMEVRGDIGLYNPGTEEYT
                     IWMSSQTPHIQRLLLTAFVTGIPEHKVRCISPDVGGAFGSKIFCYADMALVMWASKLI
                     GGRPVKWVESRRESYGSTIHGRDHITYVEIAGKRDGEITGVKVKTFANLGGRLSTIGP
                     GIPTTLYGRVLSGCYKIPNVYAEVTGVYTNTTFVDAYRGAGRPEATYVIERAMDLFAN
                     EIGMDRAAIRRKNFIPPDAFPYDNPSGLGTAVNGE"
     gene            complement(1300..1803)
                     /locus_tag="VHM48_05175"
     CDS             complement(1300..1803)
                     /locus_tag="VHM48_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2754831.1"
                     /translation="MVEHIALTVNGTPREGDVEPRRMLVQFLREDLELTGTHVGCDTS
                     QCGACTVTIDGKAVKSCTMLAVQADGANVKTIEGMQAADGTLHALQEGFWAKHGLQCG
                     FCTPGMIMAASDLLARNAEPTDDEIRHAIEGNICRCTGYHNIVAAIREAASTMRAGAA
                     APTAANV"
BASE COUNT          279 a          707 c          787 g          390 t
ORIGIN      
        1 ctcgccgttg accgccgtcc cgaggcccga cgggttgtcg tacgggaagg cgtcgggcgg
       61 gatgaagttc ttgcggcgga tcgccgccct gtccatcccg atctcgttgg cgaagaggtc
      121 catcgcccgc tcgatgacgt acgtcgcctc gggccggccg gcgccgcgat aggcgtcgac
      181 gaacgtggtg ttcgtgtaga cgccggtgac ctcggcgtag acgttcggga tcttgtagca
      241 gcccgacagg acgcggccgt agagggtggt cgggatgcct ggcccgatcg tcgagaggcg
      301 gccgccgagg ttggcgaacg tcttgacctt gaccccggtg atctcgccgt cgcgcttgcc
      361 ggcgatctcg acgtaggtga tgtgatcgcg gccgtggatc gtcgagccgt agctctcgcg
      421 gcggctttcg acccacttga ccggccggcc accgatcagc ttgctggccc acatgacgag
      481 ggccatgtcg gcgtagcaga agatcttcga gccgaaggcg ccgccgacgt cggggctgat
      541 gcagcggacc ttgtgctcgg ggatgccggt cacgaaggcg gtcagcagga gccgctggat
      601 gtgcggcgtc tggctcgaca tccagatcgt gtattcctcg gtcccggggt tgtagagccc
      661 gatgtcgccg cggacctcca tcgggttcgg gatcaggcgg tggttgacga ggcgctcgcg
      721 gacgacgacc tcggcctcgg cgagggcctt ctccgtgccg gccttgtcgc cgaccgtcca
      781 atcgaaaacg acgttgttgg gggcgttgtc gtggagctgg acggcgcccg gctgggtcgc
      841 cttctcggcg tcgacgacgg tcgccagcgg ctcgtagtcg acgtggatgc cgtcgagcgc
      901 gtcgatcgcc tgctcggccg tctcggcgac gactgctgcg acgccctcac cggtccattt
      961 cacggagtcc gtggcgagga tccggggcgt gctgatgttg ttcgtgattc cggacgcgcc
     1021 gccggccggc caggccatcg gcagcgggtt ccacgggatg tcggccccga cgatcaccgc
     1081 caggacgccg ggcatcgtct tcgccttgga cgtgtcgacc gaacggatgt tggcgtgggc
     1141 gtaagggctc cggaggatgg ccatgtggac catcccggtc agcttgatgt cgtcgagata
     1201 gcggcccttg ccggtgatga agcgcggatc ctcgacccgc ttgacggaag cgccgaggac
     1261 gtcctggacg tcggtagcca tcgctgctct cctctcggct cagacgttcg cggcggtcgg
     1321 ggcggcggcc ccggcgcgca tggtggaggc ggcttcacgg atcgcggcga cgatgttgtg
     1381 atagcccgtg cagcggcaga tgttgccctc gatcgcgtgc cggatctcgt cgtcggtcgg
     1441 ttcggcgttg cgggcgagca ggtcggaagc agccatgatc atgcccgggg tacagaagcc
     1501 gcactgcagg ccgtgcttgg cccagaaccc ttcctggagg gcgtgcaggg ttccgtcggc
     1561 ggcctgcatc ccctcgatcg tcttgacgtt ggccccgtcc gcctgcacgg cgagcatcgt
     1621 gcaactcttg acggccttgc cgtcgatcgt gaccgtgcag gcgccgcact ggctggtgtc
     1681 acagccgaca tgggtgccgg tcagctcgag gtcctcccgc aggaactgga cgagcatccg
     1741 tctcggctcg acgtccccct cgcgcggcgt tccgttgacg gtgagcgcga tgtgctcgac
     1801 cacgggtgga gcctccttcc gcgaatcggg ggccggcggt caggccggcg gggtgcgagt
     1861 gcccttgtcg atgcgtccgg gaacggcgtc catggtgacc gcgtcccggc cggcctgcaa
     1921 gccgttggtg cggcccgtga tcgaggaccg ggcccgggac ggaattcgcg cgtccccgac
     1981 cgccgcaggt gaccgcgcgc accggagttc cgaaggccgg tacttctggc cgatcgatcg
     2041 ggatcgccgc gcgttcgatt ctccgctcat gagcgctcgc acgcaccgcc tccgcgtcct
     2101 ggccatcgcc gcgacgatgg ccgtcggcct gtccggggcc gatctgtcgg cgacggccct
     2161 cgc
//
LOCUS       DATAIW010000164         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_157740, whole genome shotgun sequence.
ACCESSION   DATAIW010000164 DATAIW010000000
VERSION     DATAIW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157740"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..912
                     /locus_tag="VHM48_05180"
     CDS             184..912
                     /locus_tag="VHM48_05180"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX2754832.1"
                     /translation="MTPDEDHGIAHLAGHTGSTNHDARDGSEEADRELEPRLMPDKPR
                     TISEADRLRYEGYLAEILTALGLDLDTPGTSTTPRRFLRALFDTTSGYEGDPKLLTAF
                     PTECRGGANCEIAQVVEGPIPFFALCEHHALPFFGNVYVGYVAHEGIIGISKLTRLVH
                     VVTRRFGVQERMTYQIADELDRMMTPHGVAVYAEAHHLCTQMRGVREIHPKTRTTSYR
                     GVYVESSQLRGEFFDVSGLRRAES"
     gene            1107..1862
                     /locus_tag="VHM48_05185"
     CDS             1107..1862
                     /locus_tag="VHM48_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2754833.1"
                     /translation="MIANFVSTLDGVVAFAVDGSSGGGEVSGFSDPDRFVMGMLRAMA
                     DVVLVGAGTVRAAPTHEWTPRRVHPRSAALYATWRSRMGIASPQPTTIVATASGSLDP
                     EHPGLSAPDVPVILATTEAGAERLRAAGLAPNARIEIAGTSGQVAATRLVEIAASTGA
                     RLILCEGGPHLISDLLVAGLLDELFLTVAPQLAGRDEATHRLALVEGAAFDVATAPWA
                     DLRSVRRSGDHLFLRYRVGGRAPRQGGADPPHG"
BASE COUNT          362 a          830 c          757 g          321 t
ORIGIN      
        1 gtcccgtcag accatgcgcc gctcgtcctg gacctggacg agccgggccg cgcgttcgat
       61 ccgggctggt ccgacgcggc ggtgcggatc gctgaccggg tcgccgcacg gcgcgccggc
      121 accgaagcct gacgccgcga catccacatt cgccaacggg ccgtatcatc gatcggagga
      181 tcaatgacgc ccgacgaaga tcacggcatc gcgcacctcg cgggacacac tggctcgacg
      241 aaccacgacg cccgcgatgg gagcgaagag gcggaccgcg agctcgagcc gcggctcatg
      301 cctgacaagc cgcggacgat ctcggaagca gaccggctcc gctacgaggg ctacctcgcg
      361 gagatcctga ccgccctcgg cctcgacctc gacacgcccg gaacgagcac gaccccgcgc
      421 cgctttctcc gggcgctctt cgacacgaca tcgggctacg agggcgatcc gaagctcctc
      481 accgccttcc cgaccgagtg ccgcggcggc gcgaactgcg aaatcgcgca ggtcgtcgag
      541 ggcccgatcc cgttcttcgc gctgtgcgag caccacgctc tgccgttctt cggcaacgtc
      601 tacgtcgggt acgtcgcgca cgaagggatc atcggcatca gcaagctgac ccgcctcgtc
      661 catgtcgtga cccggcgatt cggcgtgcag gaacggatga cgtaccagat cgccgacgag
      721 ctcgaccgga tgatgacccc gcacggcgtc gcggtgtacg ccgaggccca ccacctctgc
      781 acccagatgc ggggcgtccg ggagatccac cccaagacgc gaacgacgtc gtatcgcggc
      841 gtgtacgtgg agagctccca gctgcgcggc gagttcttcg acgtcagcgg cctccgccgg
      901 gcggagtctt gacccggtcc gatctcgccc cgccgaccgg ccgcctcgaa ggaccccggc
      961 tcgaacgact gtgggacccg acggccaccg ccccgacgac gccggccggc tcgccgcgga
     1021 acttccgagg cgggcgatta cccccaggcc tccgcgatcg atacgacggc catctgcgcg
     1081 tcgcgctccg tcccgatcga ccgaccgtga tcgccaactt cgtctcgacc ctcgacggcg
     1141 tcgttgcgtt cgctgtcgat ggcagctccg gcggtggcga ggtcagcggg ttctccgatc
     1201 cggatcgctt cgtcatgggc atgctccggg cgatggccga tgtcgtcctc gtcggggcgg
     1261 gcacggtccg ggcagcgccg acgcacgagt ggacgccccg gcgagtgcat cccagatcgg
     1321 ccgcgctgta cgcgacatgg cggagccgga tgggcatcgc gagcccgcag ccgacgacga
     1381 tcgtcgcgac ggcgagcgga tcgctcgatc cagagcaccc gggtctttct gcacccgacg
     1441 tgccagtgat ccttgccacg acggaggccg gtgcggagcg cctccgggcc gcgggtctgg
     1501 cgccaaacgc gcggatcgag atcgcgggga ccagcgggca ggtagcagcg acgaggctgg
     1561 tcgagattgc cgcctcgacc ggggcgcgcc tgatcctgtg cgagggcggt ccgcacctca
     1621 tcagcgacct gctcgtcgcc ggattgctcg acgagctctt cctcaccgtt gcgccccagc
     1681 tggccggccg agacgaggcg acgcaccggc tcgcgctcgt cgagggcgca gcgttcgacg
     1741 tcgcgaccgc gccgtgggcg gacctcagat ccgtgcgccg gtccggcgac cacctcttcc
     1801 tccgctatcg ggtcggcggc cgcgcacctc gccagggagg cgccgacccg ccgcacgggt
     1861 agtcgggatc gcgagcgtgc gcgccacagc ggttctagta gtgtgtccag aacgtcggcg
     1921 accaacagag tgggcgggcc ttcgtctcgc acccaactgc atagacgtcc caatctcgaa
     1981 cccatgccac tcttgccgga gacacaatgc cccggtcgcg ccagggacca gaaagcgacg
     2041 gccgagatcc cggcaaatca tcgggctccc ctggtggcca ccgccagcga gggtcaccgg
     2101 cagaccgcct tgacgcccgg gcgtcctacg cctcggcgat taccccgatc gagatccgaa
     2161 aaccggcgtg ccgttgcacg ggaggcatga gcgtaccttg accaaccggc gcgtcgccgg
     2221 ggaaccagcg cacccattcg tcgttgaaga tgtcgaactc tgccgggtcg
//
LOCUS       DATAIW010000165        23845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_122851, whole genome shotgun sequence.
ACCESSION   DATAIW010000165 DATAIW010000000
VERSION     DATAIW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 23845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23845
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122851"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="tuf"
                     /locus_tag="VHM48_05190"
     CDS             <1..199
                     /gene="tuf"
                     /locus_tag="VHM48_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX2754834.1"
                     /translation="QFYLRTTDVTGAIGLPDGAEMIMPGDNVEMSVELITPIALEIGQ
                     RFAIREGGRTVGAGAIVTIAE"
     gene            205..513
                     /gene="rpsJ"
                     /locus_tag="VHM48_05195"
     CDS             205..513
                     /gene="rpsJ"
                     /locus_tag="VHM48_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX2754835.1"
                     /translation="MAKQRIRIRLKAYDHRLLDQSAAQIVETAQRTGADVVGPVPLPT
                     RIEKFTVLRSPFIDKDSREQFEIRTHKRLVDILDPSSKTVDALMRLSLAAGVDIEIKM
                     "
     gene            662..1285
                     /gene="rplC"
                     /locus_tag="VHM48_05200"
     CDS             662..1285
                     /gene="rplC"
                     /locus_tag="VHM48_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX2754836.1"
                     /translation="MSIGLIGRKVGVTQVFQADGTMVAVSVLAIQPNTVTRLRTDERD
                     GYVAVQLGAEPAKRLTKPEAGQLKDLPKGAQTLARIREFRVESVDDFELGQTVALGDL
                     FAEGDLVDVTGVSKGKGFAGHIKRHNFHRGPTTHGSDHHREPGSIGPGTTPGRVYKGV
                     KMAGHMGDERVTVKKLRVVRADTDKNLLLVKGSLPGPRGSLILVKKA"
     gene            1288..1911
                     /gene="rplD"
                     /locus_tag="VHM48_05205"
     CDS             1288..1911
                     /gene="rplD"
                     /locus_tag="VHM48_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX2754837.1"
                     /translation="MPQTILYDRAGKNVGSVELSDSLFAAPVNAAVLHQVVTAQLAGR
                     RTGTADTKTRGDVRGGGKKPYRQKGTGRARQGTRTAPHYRGGGVVFGPHPRSYEQRLP
                     RKMKRLALRGALTAKLGDEAIKVIDTFALEAIKTRDLAAVLGALDARGRILVVAPGRD
                     ERLELSARNLPTVEIILADSLNVVDLLKADIVVIEQPALARMEEVYA"
     gene            1908..2246
                     /gene="rplW"
                     /locus_tag="VHM48_05210"
     CDS             1908..2246
                     /gene="rplW"
                     /locus_tag="VHM48_05210"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX2754838.1"
                     /translation="MSALTAADIIMRPVISEKSIDVSTLGDRDAKAGNKYTFAVHRDA
                     NKIQIKQAIEELYAKEKVTVVSVNVLTTQAKEKSRGTKRGRIKGWTTPWRKAIVTLAP
                     GQKIEFFEGV"
     gene            2249..3076
                     /gene="rplB"
                     /locus_tag="VHM48_05215"
     CDS             2249..3076
                     /gene="rplB"
                     /locus_tag="VHM48_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX2754839.1"
                     /translation="MPLRSYKATSPGLRQMTRSTFEEITTDQPYKPLLEPQIRGSGRN
                     NAGRLTVRHRGGGEKQHYRRIDFKRDKLGVPARLATIEYDPNRSARIGLLFYRDGEKR
                     YMLLPHGLKVGDVILSGPEAEARVGNALLLANIPLGTTIHNIELVPGKGGQVVRSAGT
                     SAQLLAKEGDYATVRMPSGEMRRIPLRCMATIGQVSNLDHENQNLGKAGRARHMGQRP
                     EVRGVVMNPRDHPHGGGEGKSPTGMPPKTPWGKPAMGYRTRRAKTTGRLIVRSRHRK"
     gene            3093..3377
                     /gene="rpsS"
                     /locus_tag="VHM48_05220"
     CDS             3093..3377
                     /gene="rpsS"
                     /locus_tag="VHM48_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX2754840.1"
                     /translation="MSRSVKKGPFIELRLQGRVEEMNRRNEKKVVKTWSRASVIFPTF
                     IGHTIAVYNGKKHVPVYVTENMVGHRLGEFAHTRTYRGHGKHTERSTTLK"
     gene            3391..3729
                     /gene="rplV"
                     /locus_tag="VHM48_05225"
     CDS             3391..3729
                     /gene="rplV"
                     /locus_tag="VHM48_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX2754841.1"
                     /translation="MRVSATAKYLRGSTRKANLVVAAIKGRPVEEAAALLKFMPQHAA
                     HDVLAVLKSATANAENNHNLSAEDLIVADAQANEGPTIKRWRPRAQGRAFPIHKPMTH
                     ITIVVADREA"
     gene            3731..4414
                     /gene="rpsC"
                     /locus_tag="VHM48_05230"
     CDS             3731..4414
                     /gene="rpsC"
                     /locus_tag="VHM48_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX2754842.1"
                     /translation="MGRKVHPYGFRLGVSRTWTAKWYADKEYTDLLKEDIGIRKLVEK
                     RLANASVSGVEIERGINHVTVTIHTAKPGIVIGKGGANVEILRQQIGGLTKRRVKLEI
                     KEIRQPELDAHLVAMNIAGQLSRRIAFKKAMKQAIQRSMKAGAKGVRIAVAGRLGGSE
                     MGRREWDREGRIPLGTLRADISYGQIHAHTTYGRIGVKVWIYRGDFAPERQAPRDLNP
                     ATAVVGQGN"
     gene            4419..4856
                     /gene="rplP"
                     /locus_tag="VHM48_05235"
     CDS             4419..4856
                     /gene="rplP"
                     /locus_tag="VHM48_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX2754843.1"
                     /translation="MLMPKRVKHRKVMRGRMSGMAKGGHTVAFGDYGLATQEPAWITS
                     RQIEAARRAMTRSVKRGGKIWIRIFPDKPATKKPAETRMGSGKGAPDHWVAVVRPGRI
                     LFEMSGVDHESAVEAMRLASHKLPVATRVVVRDNVKEAADGHR"
     gene            4843..5043
                     /gene="rpmC"
                     /locus_tag="VHM48_05240"
     CDS             4843..5043
                     /gene="rpmC"
                     /locus_tag="VHM48_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX2754844.1"
                     /translation="MDIVKVRELSDTELENALREAKQDLWQARFALSTRQLKDYSTIP
                     QTRRTIARILTVQRERKLGQTA"
     gene            5057..5323
                     /gene="rpsQ"
                     /locus_tag="VHM48_05245"
                     /pseudo
     CDS             5057..5323
                     /gene="rpsQ"
                     /locus_tag="VHM48_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            5467..5835
                     /gene="rplN"
                     /locus_tag="VHM48_05250"
     CDS             5467..5835
                     /gene="rplN"
                     /locus_tag="VHM48_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX2754845.1"
                     /translation="MIQPQSRLRVADNTGARSIQCIRVMGRSLKTTAGVGDVIIASVK
                     QAIPNAAVKKGDVVRAVVVRTAKEYGRPDGSYIRFDENAAVLINNQGNPRGTRIFGPV
                     ARELRDRNYMKIVSLAPEVL"
     gene            5835..6287
                     /gene="rplX"
                     /locus_tag="VHM48_05255"
     CDS             5835..6287
                     /gene="rplX"
                     /locus_tag="VHM48_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2754846.1"
                     /translation="MAAARPRRQVTKVPEIRRGDTVVVLTGKDAGKRGTVERLVRDPQ
                     GLKKTRTKYGSRLDKMSPLVTTAVVVEGVNVSKRHTKPRQSAGTNDRTPKITQGGILD
                     LAQPMSIGKVMVVCGHCDKPTRIAHRTLDNGRRVRVCRHCGEQLETRS"
     gene            6284..6826
                     /gene="rplE"
                     /locus_tag="VHM48_05260"
     CDS             6284..6826
                     /gene="rplE"
                     /locus_tag="VHM48_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2754847.1"
                     /translation="MTARLQARYTADVVPVLQKQFEYGNPMQVPKLAKIVVNIGLGEA
                     LTNAKALDAAVGDLTKIVGQKPIVTKAKRSIAQFRLRTGNTIGAKVTLRGERMWDFLD
                     RLTTLALPRIRDFRGVPGKSFDGRGNYTLGLREQLAFPEIEYDKVDRLRGLEISIVTT
                     AKTDEESKRLLELLGMPFAS"
     gene            6845..7030
                     /locus_tag="VHM48_05265"
     CDS             6845..7030
                     /locus_tag="VHM48_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2754848.1"
                     /translation="MAKKSLIAKAKREPKFKVRGYSRCFVCGRSRAYMRRFALCRICF
                     RERALLGELPGVTKSSW"
     gene            7039..7437
                     /gene="rpsH"
                     /locus_tag="VHM48_05270"
     CDS             7039..7437
                     /gene="rpsH"
                     /locus_tag="VHM48_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2754849.1"
                     /translation="MNISDPIADMLTRVRNASRARHTEVVVPASRTKREIARILKDEG
                     FIADVREEQDGPTIVLHLTLKYVDGKVPVVSGLKRISKPGLRVYARKTDIPRVLGGLG
                     IVIVSTSQGIMTGAQARKAELGGEVLAFVW"
     gene            7448..7999
                     /gene="rplF"
                     /locus_tag="VHM48_05275"
     CDS             7448..7999
                     /gene="rplF"
                     /locus_tag="VHM48_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2754850.1"
                     /translation="MSRIGRLPIAVPTTVDVTIEGRRVTVKGPRGTLTRDLHPDMTVS
                     REEGNLVVTRPTEQKTHKQLHGLTRTLVNNMVIGVTDGYRKGLEITGVGYRATKVGEK
                     LQLNLGYSHQIEIDPPSGISFEVENPTRLAVVGIDKELVGQIAAKVRSTRKPEPYKGK
                     GVRYAGEKIRRKAGKAGKIGGKK"
     gene            8021..8383
                     /gene="rplR"
                     /locus_tag="VHM48_05280"
     CDS             8021..8383
                     /gene="rplR"
                     /locus_tag="VHM48_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2754851.1"
                     /translation="MTQVASRGAARRKRHDRIRLHLEGTESRPRLAVFRSNIHIYAQV
                     IDDASGRTLAAASTLEKELRGAKSTKTDEAAVVGRLLAERAKTAGVERVVFDRAGFRY
                     HGRIKSLAEAAREAGLEF"
     gene            8399..8941
                     /gene="rpsE"
                     /locus_tag="VHM48_05285"
     CDS             8399..8941
                     /gene="rpsE"
                     /locus_tag="VHM48_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2754852.1"
                     /translation="MARIDPNKYALEERVVQINRVAKVVKGGRRFSFSAIVVVGDGAG
                     TVGAGLGKAGEVPEAIRKGVEDAKKNLIRIPMVGTTIPHEVRQVFSASRVMLKPASQG
                     TGVIAGGAVRAVVEAAGIRDILAKTLGSTNPVNVTRCTIEALRSLHSAEELSARRGVK
                     LVSRISGQPAAVATEAGDGR"
     gene            8931..9140
                     /gene="rpmD"
                     /locus_tag="VHM48_05290"
     CDS             8931..9140
                     /gene="rpmD"
                     /locus_tag="VHM48_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2754853.1"
                     /translation="MADKLRVTQIKSTISHIARNRATVRALGLKRIGHTVEIPDNPAT
                     RGMVRQVRFLVSVEEVGGADAQEKA"
     gene            9137..10072
                     /gene="rplO"
                     /locus_tag="VHM48_05295"
     CDS             9137..10072
                     /gene="rplO"
                     /locus_tag="VHM48_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2754854.1"
                     /translation="MKLHDLRPPDGSNTARTRVGRGIAAGKGKTAGRGTKGQKARAGG
                     SIPPWFEGGQTPLHMRIPKLRGFKNRAKIDYEVVNVGDIAARIDAGAFATAGTTPKKG
                     EQMTINADILRAVGLVRNVKKPLKILGNGDLATALFVVADAYTKSATAKIEGAGGTVN
                     VLEIPVESRPALGLDSDGVAIERTARVPRTAAGRDAADREVDARARRDSDAAEAEAAA
                     TAASAEKAERPKKAPKSADPSKAKAEAPDAPAEASTPAPVADAADVTDAPDGAASADE
                     PADAPDAVVDDAAAAPDGAVDADGDAPLPTGDESA"
     gene            10079..11359
                     /gene="secY"
                     /locus_tag="VHM48_05300"
     CDS             10079..11359
                     /gene="secY"
                     /locus_tag="VHM48_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2754855.1"
                     /translation="MFESLLNAFRAPDIRRRLLYVLGILIVFRFLAHVPVPGVNQDQL
                     ASYLKDNALFGVLDLLSGGGLSSFSVVALGVNPYINASIIMQLMSGVIPSLQALQREG
                     EYGRNKINQYTRYLSVPMALLQSYGFLALLNSQGILSSGFDLANGATLTQIATLTAGT
                     MTLMWLGELITEKGIGNGISFIIFAGIVSRTPGAIGGFLATPNLPLIIGFTIIAIVSV
                     AVIIYIQEGQRRIPIQYASRVRGRRMYQGGQTFLPLRVNQAGVIPIIFAISILLFPTQ
                     IASYFTGSEVDWVKSIANAIVGFFNPRTIPYVALYFALTVGFTYFYTAFTFKPDDTAE
                     QLRKNGGFIPGIRPGRPTADYLARVVTRITIAGALFLGIVATLPTLLGLIFPSLSGIA
                     LGGTGLLIVVSVIVETMKQVEAQLLMRNYEGFIR"
     gene            11412..12098
                     /locus_tag="VHM48_05305"
     CDS             11412..12098
                     /locus_tag="VHM48_05305"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX2754856.1"
                     /translation="MTVVVLLGSPGAGKGTQAPVLSRRLGVPVLASGDLLRTALAKGT
                     ALGREADRYMSRGQLVPDETIVRVFLDRLEQPDAADGAILDGFPRTRIQAEALDRALI
                     EAGRRVDRAIYIDVPTEDLVRRMANRRICAANGHVYNLASNPPLVAGICDLDGSELVQ
                     RVDDNELTARARMSEQIPPLLEVVDHYRAAGVLQVVDGRQPIAELSENIVAAGATPVP
                     DHAPDWQPAG"
     gene            12098..12856
                     /gene="map"
                     /locus_tag="VHM48_05310"
     CDS             12098..12856
                     /gene="map"
                     /locus_tag="VHM48_05310"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2754857.1"
                     /translation="MVTRKSRREIEKMRAAGRIVAEVLALVESELRPGVSTGHLDRLA
                     ERHIRSAGAVPSFMGYGHRSNPFPASLCISIDDEVVHGIPGERLIRDGQIVSVDAGAI
                     FDGWHGDGARTFVVGEAGSEVEALVDTTRLAMMAGIAAAVPGANLGDISGAIEDAATP
                     GGYGIVRQFVGHGIGTEMHQEPQLPNYRTGVRGMKLVPGICLAIEPMLTLGGPEVSIK
                     ADGWTVVTRDGSLAAHFEHTIAVTDNGPEILTTI"
     gene            13045..13263
                     /gene="infA"
                     /locus_tag="VHM48_05315"
     CDS             13045..13263
                     /gene="infA"
                     /locus_tag="VHM48_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2754858.1"
                     /translation="MAKRDAIEVEGTVIEPLPNTMFAVELENGHRVLAHISGKLRMNF
                     IRILPGDRVRVELSPYDLTRGRITYRLK"
     gene            13311..13424
                     /gene="rpmJ"
                     /locus_tag="VHM48_05320"
     CDS             13311..13424
                     /gene="rpmJ"
                     /locus_tag="VHM48_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2754859.1"
                     /translation="MKVRASVKARCDNCKVIRRHGTVMVICTNPKHKQRQG"
     gene            13427..13798
                     /gene="rpsM"
                     /locus_tag="VHM48_05325"
     CDS             13427..13798
                     /gene="rpsM"
                     /locus_tag="VHM48_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2754860.1"
                     /translation="MARIASVDIPREKRVEIALTYIFGIGLPTAQKILTQTNVNPDTR
                     VRDLTEEQVNRLRELIDRRYKVEGDLRREVALNIKRLIEIGSYRGMRHRRNLPARGQR
                     TKTNARQRRGPKKTVGVRRKK"
     gene            13819..14214
                     /gene="rpsK"
                     /locus_tag="VHM48_05330"
     CDS             13819..14214
                     /gene="rpsK"
                     /locus_tag="VHM48_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2754861.1"
                     /translation="MAERKRVVKQRRKERKNVPQGQVHIQATFNNTIITITDLTGAVI
                     SWGSAGVAGFKGSRKSTPYAAALSADGAARRAMEHGMRQVEVYVKGPGAGREQAIRSL
                     QAAGLEVSAITDVTPIPHNGCRAPKRRRV"
     gene            14230..14856
                     /gene="rpsD"
                     /locus_tag="VHM48_05335"
     CDS             14230..14856
                     /gene="rpsD"
                     /locus_tag="VHM48_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2754862.1"
                     /translation="MARYTDSVCRLCRREGAKLYLKGSRCYTKKCAFERRPSPPGQHG
                     VRRRKVGDYGLQLREKQKVRRVYGVLERQFRNYFDRAENRPGVTGESLLRTLELRFDN
                     VVYRMGFASSRAQARQLVGHGHFSVNGVPTNIPSYQLKAGDRIEVRENRRAREPFKLA
                     KETLRSHQAPEWLSIDAAKLSGSVADLPRRDQMPLDLNEQLVVEYYSR"
     gene            14866..15858
                     /locus_tag="VHM48_05340"
     CDS             14866..15858
                     /locus_tag="VHM48_05340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX2754863.1"
                     /translation="MIELENPQIEPVEEAGTYAKYEAGPLQAGYGVTLGNALRRVLLS
                     SLDGAAVTSIQIRDVYQEFSTIPGVKEDVTQIVLNVKKLRMKSFATHPVQLRLIKSGA
                     GQVTAADIAESADVEIINPELVLMTLDTDDVTIEMDLTVERGVGYLGAERSEQLPIGV
                     IAIDAIFTPVRKVNYWVEGMRVGQMTNYDKLTIEIETDGAITPEEALSRSAEILVNQF
                     RPFVTIGMAAAVGEQATPGLAQLPPNMLDMPIEELDLPMRAYNSLKRNNIVKVGQLLQ
                     LKDDDLLRMRNFGKKSLDEMKERLRMRGFIIPETESDGLEDAAFEDEGDDEGES"
     gene            15860..16213
                     /gene="rplQ"
                     /locus_tag="VHM48_05345"
     CDS             15860..16213
                     /gene="rplQ"
                     /locus_tag="VHM48_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX2754864.1"
                     /translation="MAHCIGGRKLSRKQGPRLALFKNLTVSILRYERVQTTEAKAKEI
                     QGRVEKMITLAKRNDLTARRAVVAAFPNEPLVVTKLFDEIAPKYADRTSGYTRIVKIG
                     LRRGDAAPIVQIELV"
     gene            16235..17005
                     /gene="truA"
                     /locus_tag="VHM48_05350"
     CDS             16235..17005
                     /gene="truA"
                     /locus_tag="VHM48_05350"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX2754865.1"
                     /translation="MRYRATVEYDGTDFAGFQAQRPGIRTVQGELEAALARLGDGVRQ
                     PVDGAGRTDAGVHALGQVIAFTFTGRLTAPRLEAALNGQLPPDIAVRDVRAVAPEFHP
                     RRAARYREYRYTVWNGPRSPLHERHALGIRAPLDIAAMARAGQAFIGPHDFSAFGVVI
                     GGRSPVRNVHSVRIGRKGQLVTIDVRANAFLRGMVRRMVAVLIEVGHGKMTETEVREA
                     IAARTPARNGVSAPARGLCLRRVVLGRRLEMETTDGDD"
     gene            17012..17446
                     /gene="rplM"
                     /locus_tag="VHM48_05355"
     CDS             17012..17446
                     /gene="rplM"
                     /locus_tag="VHM48_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX2754866.1"
                     /translation="MSTKTYTVRASEIERRWYVVDAADETLGRLASRIARVLEGKHKP
                     TWTPNLDSGDHVIVLNAGRITVTSNKLESKLYIRHSGHPQGYKEETLGHLLERRPEEV
                     IRRAVKGMLPRNRLGVQQLRKLKVYAGTDHPHQAQRPEPLAR"
     gene            17457..17855
                     /gene="rpsI"
                     /locus_tag="VHM48_05360"
     CDS             17457..17855
                     /gene="rpsI"
                     /locus_tag="VHM48_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2754867.1"
                     /translation="MTVPSFFSGTGRRKTAIARVRLLPGEGEIVVNGRSLEEHFGNAV
                     NESDLRMPFRVTGTEGHYNAMIKVEGGGVTGQAGAIRHGIARALLEVDEATNRLPLRQ
                     AGLLTRDPRMKERKKYGLKRARKAPQYTKR"
     gene            18048..19013
                     /gene="argF"
                     /locus_tag="VHM48_05365"
     CDS             18048..19013
                     /gene="argF"
                     /locus_tag="VHM48_05365"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2754868.1"
                     /translation="MSTTAPDTSLRGRDLLSVADLSTAEVGRIFDRAVALKAEDRARR
                     ATGVAAATPLTGLTLAMLFQKPSLRTRVTFEAGMNQLGGHAIYLPEDTVMGARESVRD
                     VARNLERFVDAIVARTGPHEVVVELAAQASIPVINALTLREHPCQALADLFTMRERFG
                     DLRGRVLAFVGDGNNVYHSLALLGAILGLEVRLAHPADYGPNPRIVERAIELAAAAGG
                     RLVFDDDPTTIVRGADVVYTDAWTSMGQEAETEARRDAFARYRVDEALLDAAGPDTAV
                     MHCLPAHRGEEITSAVMDGPRSIIWDQSENRLHVQKALLVEVLAG"
     gene            19010..20212
                     /locus_tag="VHM48_05370"
     CDS             19010..20212
                     /locus_tag="VHM48_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2754869.1"
                     /translation="MSGSRRGSGKRAPRPGGATANGSLYERYKDALRRGHVAALRGRN
                     DAAVDAYGEAASIAPDRALPHTSIGGILVKMNRLDEAVVAYERALAMAPRDEGALRGL
                     ADVHVRTGRRTEAAATLDRLADSLDAAGRLADATDAARRALELAESRARRRQVEGFAG
                     RLRTSSSGDETAQRALDQALRLLEPPSRAVDAVEDDALLVDGATDDDRPDTEARPAAE
                     EETAEVVEEPTAEAAPVAEPTGEPEPEPEPEPEPEPEIVAEPEPEPEPAEPEPEPEPE
                     PVTEPAGLGIALGAAAEAALHAGDFAAAHDGLLAAARAHRRAGRLSAAVDACYLAIGL
                     APSDPDLHLLLAELYLDRGWRGQAIEKLLLLGRLADLDDDAAVRQRLCDLVTARLADE
                     PRVADLCA"
     gene            20335..21279
                     /gene="cdaA"
                     /locus_tag="VHM48_05375"
     CDS             20335..21279
                     /gene="cdaA"
                     /locus_tag="VHM48_05375"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX2754870.1"
                     /translation="MPQLLASIVDQIDLTSLVDIGITALLIYWLFSLIRGTRAVSLVI
                     GVSVLFAVYAAARAFDLRLLTQILQTLAVVGLFALVVVFQPELRRALERIGRVGSFNW
                     LFSPTEQRAVEHVADEVARAAALLSADGHGALIVLERDTGLEEVAETGVMLHADISVD
                     LLRTIFAPRTPLHDGAVIIREETILAAAALLPLAEMTIHTERFGTRHRAALGITEQTD
                     AVVVVVSEENSQVSLVERARIVRNLSEPQLAKAIKALLAPTDGRGRLGRRVLPDAQTG
                     GRAPRLSDLGRFVGRGRPPVVSPPPRADDDPAPARAPR"
     gene            21276..22508
                     /locus_tag="VHM48_05380"
     CDS             21276..22508
                     /locus_tag="VHM48_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX2754871.1"
                     /translation="MTRLLGAIVYNWPLKLMAIVLATLLYAGLVVSQSTFEIPAALQI
                     HPLNQPLTAVILGNLPYVSRVRYVANGDVGAGPTPDSFRATIDLSGVNPEAGSTYVKI
                     DVSSNDPRFLAVDWEPRGINVQLDPFRSYQVPVRVNKGTVPDNLDVRPAELSVSTVTV
                     SGPDSVVKFVVEARADVLIDPSGTPVDRDVPLIPVDQLGNRLTPVQVEPASVHVTIAV
                     FNNSRKKVLAVNPIVTGTPPQGYVVDTITVTPSTVTVEGNADQLSALVRADTKAISIG
                     TATGTIDTDVDLDLPAGVLPIGSKSVHLTITLKVETGTKAFAAALVPVGRQPGLEYRF
                     STLNILVNVAGPLADLDRLDPSSFTVPLDVTGLGVGVHEVMPTPNLQAGLRLLNVDPA
                     TVTVTITAPASPAPSAGG"
     gene            22511..23845
                     /gene="glmM"
                     /locus_tag="VHM48_05385"
     CDS             22511..23845
                     /gene="glmM"
                     /locus_tag="VHM48_05385"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX2754872.1"
                     /translation="MPRLFGTDGIRGVANVELKPTLAYALGRATAYQLVGQGGRLVVG
                     QDTRRSGDMFVSAITAGATSLGADVHIAGCVPTPALAFLAGTGDFAAGIMVSASHNPA
                     DDNGLKVLDSKGLKLDDGIEEEIEQLIWRTEELGSVGNAEIGRAIVDPGLLDRYREHR
                     LGLAGSISAVGLRIVLDCANGSGGVVGPEILAATGASVEVIHNEPDGININVASGATS
                     PWSLAAAVKERGADVGFALDGDADRLIAVDATGTIVDGDQVLGILALDRLDRGVLPEG
                     ALVVSVLSNGGLQAAVEAAGGQVIRTPVGDKYILEGMQVSGAGLGGEKSGHVIVLEHT
                     TSGDGIVTALEVLRVMTRAGRPLAELAERIQMLPQQQRAVRARHRDHWEGDAAIQRAI
                     RAAEERLGAGGRVLVRPSGTEPALRVMIEGPDADLVAELADSIATLAGERLN"
BASE COUNT         4067 a         8571 c         7924 g         3283 t
ORIGIN      
        1 gcagttctac ctgcgcacga ccgacgtgac cggggcgatc ggcctgcccg acggggccga
       61 gatgatcatg cccggcgaca acgtcgagat gagcgtcgag ctgatcaccc cgatcgccct
      121 cgagatcggc cagcgcttcg ccatccgcga gggtggccgg accgtcggcg ccggcgcgat
      181 cgtcacgatc gccgagtaag cgagatggca aagcagcgca tccggatccg cctcaaggct
      241 tacgatcacc gactgctcga ccagtcggcg gcccagatcg tcgagacggc gcagcgcacc
      301 ggcgccgacg tcgtcggacc ggtcccgctg ccgacccgga tcgagaagtt caccgtcctg
      361 cgttctccgt tcatcgacaa ggacagccgg gagcagttcg agatccgcac ccacaagcgg
      421 ctcgtcgaca tcctggatcc gagctcgaag acggtcgacg ccctgatgcg actctcgctt
      481 gccgcgggcg tcgacatcga gatcaagatg tgatgcacga gcgatctgaa ccagcaacct
      541 cagcggggat gtgccgctcg ctcaccgaag tccgaggcgg agctccgcga cgccatcggc
      601 ctgaggtgcg agcgcgcgca ggagccaacg cgggcatgag ccgcatgagc gagcgagtcc
      661 gatgagtatt ggattgatcg gccgcaaggt cggagtgacc caggtgttcc aggcggacgg
      721 cacgatggtg gccgtcagcg tcctggccat ccaaccgaat accgtgaccc gcctccggac
      781 cgacgagcgc gacggctacg tggccgtcca gctcggggcc gagccggcga agcgcctgac
      841 caagcccgag gcgggtcagc tcaaggacct gccgaagggc gcccagaccc tcgccaggat
      901 ccgtgagttc cgggtcgaga gcgtggacga cttcgagctc ggccagaccg tcgccctggg
      961 cgacctcttc gccgagggcg acctggtcga tgtgaccggc gtctcgaagg gcaagggctt
     1021 cgcgggccac atcaagcgcc acaacttcca ccgcgggccg acgacccacg gctcggacca
     1081 tcaccgcgag cccggctcga tcggtcccgg caccacgccg ggtcgcgtct acaagggcgt
     1141 gaagatggcc ggccacatgg gcgacgagcg cgtgaccgtc aagaagctgc gggtcgtccg
     1201 cgccgacacg gacaagaacc tcctcctcgt gaaggggtcc ctgcccgggc cgcgcggctc
     1261 gctcatcctc gtgaagaagg cctgacgatg ccgcagacca tcctctacga ccgagccggc
     1321 aagaacgtcg gcagcgtcga gctgtccgat tcgctcttcg ctgcgccggt caacgcggcc
     1381 gtcctccacc aggtggtgac ggcccagctg gccggccgcc ggaccggcac ggccgatacg
     1441 aagacccgtg gcgacgtccg cggcggcggc aagaagccgt accgccagaa gggcaccggc
     1501 cgcgcccgcc agggcacccg gaccgcgccc cactatcgcg gtggcggcgt cgtgttcggt
     1561 ccccatccgc ggagctacga gcagcggctg ccgcgcaaga tgaagcgact cgccctccgc
     1621 ggcgccctga ccgccaagct cggcgacgag gcgatcaagg tgatcgatac gttcgccctc
     1681 gaggcgatca agacccgcga cctggccgcc gtcctcggcg cgctcgacgc ccgcggccgg
     1741 atcctcgtcg tcgcccccgg gcgtgacgag cggctcgagc tgtcggcccg caacctgccg
     1801 acggtcgaga tcatcctggc cgactcgctc aacgtggtcg acctgctcaa ggccgacatc
     1861 gtggtcatcg agcagccggc cctcgcccgg atggaggagg tctacgcgtg agcgccctga
     1921 ccgccgccga catcatcatg cgacccgtca tcagcgagaa gtcgatcgac gtctcgaccc
     1981 tcggtgatcg cgatgccaag gccggcaaca agtacacctt cgccgtccac cgcgacgcga
     2041 acaagatcca gatcaagcag gcgatcgagg agctgtacgc caaggagaag gtcaccgtcg
     2101 tgagcgtcaa cgtgctcacg acccaggcca aggagaagag tcgcggcacc aagcgcggcc
     2161 ggatcaaggg ctggacgacc ccgtggcgca aggcgatcgt caccctggcc cccggccaga
     2221 agatcgaatt cttcgagggg gtctgacgat gccgcttcgt tcctacaagg ccacctcgcc
     2281 gggtctccgg cagatgaccc ggtcgacctt cgaggagatc acgaccgacc agccgtacaa
     2341 gccgctcctc gagccgcaga tccgcggctc cggccggaac aacgccggtc gattgaccgt
     2401 ccgccatcgc ggcggcggcg agaagcagca ctatcgccgg atcgacttca agcgcgacaa
     2461 gctcggcgtg ccggctcggc tcgcgacgat cgagtacgac ccgaaccgct cggcccggat
     2521 cggcctcctc ttctaccgtg acggcgagaa gcgctacatg ctcctgcccc acggcctgaa
     2581 ggtcggcgac gtgatcctct ccggcccgga ggccgaggcg cgggtcggca acgccctgct
     2641 gctcgccaac atcccgctcg gcacgacgat ccacaacatc gagctcgtcc ccggcaaggg
     2701 cggccaggtc gtccggtccg ccggcacgtc ggcccagctc ctcgccaagg aaggcgacta
     2761 cgcgacggtc cgcatgccgt cgggcgagat gcgccggatc ccgctgcgct gcatggccac
     2821 gatcggccag gtcagcaacc tcgaccacga gaaccagaac ctcggcaagg ccggccgcgc
     2881 ccgccacatg ggccagcgac cggaagtccg cggcgtcgtc atgaacccgc gcgaccatcc
     2941 gcacggcggc ggagagggca agtccccgac ggggatgccg cccaagacgc cgtggggcaa
     3001 gccggcgatg ggctaccgga cccgtcgcgc caagacgacc ggacgcctga tcgtccgatc
     3061 gcgtcatcgc aagtaaggag ggaggtagct agatgtctcg ttctgtcaag aagggcccgt
     3121 tcatcgagct ccgcctccag gggcgggtcg aggagatgaa ccggcgcaac gagaagaagg
     3181 tcgtcaagac ctggtcccgg gcgtcagtga tcttcccgac cttcatcggc cacaccatcg
     3241 ccgtctacaa cggcaagaag cacgtcccgg tctatgtgac cgagaacatg gtcggtcatc
     3301 gcctcggcga gtttgcccac acccgcacct atcgcgggca cggcaagcac accgagcgat
     3361 cgacgaccct gaagtaggag ccgaggaccc gtgcgcgttt ccgccactgc caagtacctc
     3421 cgcggctcca cccgcaaggc caatctcgtc gtcgcggcga tcaagggccg gccggtcgag
     3481 gaggccgccg ccctgctcaa gttcatgccg cagcacgcgg cccacgacgt gctggccgtc
     3541 ctcaagagcg ccacggccaa cgccgagaac aaccacaacc tgtccgccga ggacctgatc
     3601 gtcgccgatg cccaggcaaa cgagggcccg acgatcaagc gctggcggcc gcgggcccag
     3661 ggtcgggcat tcccgatcca caagcccatg actcacatca cgatcgtcgt cgcggaccgg
     3721 gaggcctgat atgggcagaa aagtccatcc gtacggcttc cgcctgggtg tctcgcgcac
     3781 ctggaccgcg aagtggtacg cggacaagga atacaccgac ctcctcaagg aggacatcgg
     3841 catccgcaag ctcgtcgaga agcggctcgc caacgcgagc gtgtcgggtg tcgagatcga
     3901 gcgaggcatc aaccacgtga cggtcacgat ccacacggcc aagcccggga tcgtcatcgg
     3961 caagggtggc gccaacgtcg agatcctccg ccagcagatc ggcggactca cgaagcgtcg
     4021 ggtcaagctg gagatcaagg agatccgcca gccggagctc gacgcccacc tcgtggcgat
     4081 gaacatcgcc ggccagctgt cgcgccggat cgcgttcaag aaggcgatga agcaggccat
     4141 ccagcggtcg atgaaggccg gcgccaaggg cgtccggatc gcggtcgccg gacgcctcgg
     4201 tggctccgag atgggtcgcc gcgagtggga ccgcgaaggt cggatccccc tcgggacgct
     4261 tcgcgccgac atcagctatg gccagatcca cgcccacacg acgtacggtc ggatcggggt
     4321 caaggtctgg atctatcgcg gcgacttcgc cccggagcgt caggccccac gtgacctgaa
     4381 cccggccacg gccgtcgtcg gccaggggaa ctgaaccgat gttgatgccg aagcgggtca
     4441 agcaccgcaa ggtgatgcgc ggccggatgt ccggcatggc caagggcggt cacacggtcg
     4501 cgttcggcga ctacgggctg gccacccagg agccggcctg gatcaccagc cgccagatcg
     4561 aggccgctcg acgcgccatg acccgctcgg tcaagcgcgg cggcaagatc tggatccgga
     4621 tcttcccgga caagccggcc accaagaagc cggccgagac ccggatgggc tcgggcaagg
     4681 gcgccccgga tcactgggtc gccgtcgtcc ggccgggccg gatcctgttc gagatgtccg
     4741 gcgtcgacca cgagtcggcg gtcgaggcga tgcgcctcgc cagccacaag ttgcccgtcg
     4801 cgacgcgggt cgtcgtccgc gacaacgtga aggaggcggc cgatggacat cgttaaggtc
     4861 cgcgagctgt ccgacaccga gctcgagaac gccctccgcg aggcgaagca ggacctctgg
     4921 caggcccgct tcgcgctttc gacccgtcaa ctcaaggact acagcacgat cccgcagacg
     4981 cggcgcacga tcgcgcggat cctgaccgtc cagcgcgagc gcaagctcgg ccagacggcg
     5041 tgagggagat cacgcgatga caggagcaac cccccaggtg cagaacaagc ggaagacgaa
     5101 ggtcggccgg gtcgtcagcg acaagatgga caagacgatc gtcgtgtccg tcgagcgcct
     5161 ggcgcgccac cctctctaca agcgggtcat ccggctgacg acgaagttca aggctcacga
     5221 cgagctgaac gacgcgcaca ccggcgacac cgtcgtgatc gaggagtcgc ggccgctctc
     5281 ggccacgaag cgctggaggc tggtctccgt cgtccagcgc gccggcgagc acggcaaccc
     5341 cgcggacatc gtggtcgacg aggccgagac gtccgaggcg atccacgccg ccgcccatcc
     5401 cggtcgtcac gcggccgacg aggatgatgc gaccgaggcc gccgagggga gcggggagac
     5461 gccggcatga tccagccgca gtcccgcctc cgcgtcgccg acaacaccgg cgcccgatcg
     5521 atccagtgca tccgggtcat gggccgctcg ctcaagacca cggccggcgt cggtgacgtc
     5581 atcatcgcca gcgtcaagca ggccatcccg aacgccgccg tcaagaaggg cgatgtcgtc
     5641 cgggcggtcg tcgtccggac ggccaaggaa tacggccgac ccgacggcag ctacatccgc
     5701 ttcgacgaga acgcggccgt cctgatcaac aaccagggca acccgcgcgg cacccggatc
     5761 ttcggcccgg ttgcccgcga gctgcgcgac cgcaactaca tgaagatcgt gtccctcgcc
     5821 ccggaggtgc tgtgatggcc gcagcccggc cccgccgcca ggtcacgaag gttcccgaga
     5881 tccgccgcgg cgacacggtc gtcgtgctga ccggcaagga cgccggcaag cgcggcaccg
     5941 tcgagcgcct cgtccgcgat ccgcagggcc tcaagaagac ccggaccaag tacggcagcc
     6001 gtctcgacaa gatgtcgccg ctcgtcacga ccgcggtcgt cgtcgagggc gtcaacgtgt
     6061 ccaagcgcca caccaagccg cgccagagcg cgggcacgaa cgaccggacg ccgaagatca
     6121 cccagggcgg catcctcgat ctcgcccagc cgatgtcgat cggcaaggtc atggtcgtct
     6181 gcgggcactg cgacaagccg acccggatcg cccatcggac gctcgacaac ggccgccgcg
     6241 tccgcgtctg ccgtcactgc ggcgagcagc tggagaccag gtcgtgaccg cccgactcca
     6301 ggcgcgctac acggccgacg tcgtgccggt gctccagaag cagttcgaat acggcaaccc
     6361 gatgcaggtc ccgaagctcg ccaagatcgt cgtcaacatc ggtctgggcg aggccctgac
     6421 caatgccaag gcgctcgatg cggcagtcgg cgacctgacg aagatcgtcg gccagaagcc
     6481 gatcgtgacc aaggccaagc gctcgatcgc tcagttccgt ctccggacgg gcaatacgat
     6541 cggcgccaag gtcaccctcc gcggcgagcg gatgtgggac ttcctcgacc gcctgaccac
     6601 gctggccctg ccccgcatcc gcgacttccg cggcgtgccc ggcaagtcct tcgacggccg
     6661 cggcaactac accctcgggc tccgcgagca gctcgccttt cccgagatcg aatatgacaa
     6721 ggtcgaccgt ctccgcgggc tggagatcag catcgtgacg acggcgaaga ccgacgagga
     6781 gagcaagcgt ctgcttgagc tcctcggcat gcccttcgcc tcgtagcgct gggggaggaa
     6841 gacgatggcc aagaagtcgt tgatcgccaa ggcaaagcgc gaaccgaaat tcaaggttcg
     6901 cggctacagc cgctgcttcg tgtgcgggcg ctcgcgcgcc tacatgcggc ggttcgccct
     6961 gtgccggatc tgcttccggg agcgcgccct gctgggcgag ctgcccggcg tcacgaagtc
     7021 gagctggtag gaatccagat gaacatctcc gatccgattg ccgacatgct gacccgcgtg
     7081 cggaacgcct cccgggcgcg ccacaccgag gtcgtcgtcc cggcgtcgcg gaccaagcgg
     7141 gagatcgccc ggatcctcaa ggacgagggc ttcatcgccg acgtccgcga ggagcaggac
     7201 ggcccgacca tcgttctcca tctgaccctc aagtacgtcg acggcaaggt gccggtcgtg
     7261 tccgggctga agcggatcag caagcccggc ctgcgggtgt atgcccgcaa gaccgacatc
     7321 ccgcgcgtcc tcggcggcct gggcatcgtc atcgtcagca cgagccaggg gatcatgacc
     7381 ggcgcgcagg cccgcaaggc cgagctcggc ggcgaagtcc tcgcgttcgt ctggtaggcg
     7441 gaacgacatg tcacgtatcg gtcgcctccc gatcgccgtc cccaccaccg tcgatgtgac
     7501 gatcgagggc cggcgggtca cggtcaaggg ccccaggggg accctgaccc gggacctcca
     7561 ccccgacatg accgtcagcc gcgaagaggg caacctcgtc gtcacccggc cgacggagca
     7621 gaagacccac aagcagctgc acggcctgac ccggaccctc gtgaacaaca tggtcatcgg
     7681 tgtgaccgac ggctatcgca agggactcga gatcaccggc gtcggctatc gcgcgacgaa
     7741 agtcggcgag aagctccagc tcaacctggg ctacagccac cagatcgaga tcgatccgcc
     7801 ctcgggcatc agcttcgagg tcgagaaccc gacccgcctc gcggtcgtcg gcatcgacaa
     7861 ggagctcgtc ggccagatcg ccgcgaaggt ccgctcgaca cgcaagccgg agccgtacaa
     7921 gggcaagggc gttcgctacg ccggcgagaa gatccggcgc aaggccggca aggccggcaa
     7981 gatcggcggg aagaagtaac ccagtgaagg aacgagcacg atgacgcagg tcgccagccg
     8041 cggcgccgct cgacgcaagc gccacgaccg gatccgcctc cacctggagg ggaccgagtc
     8101 gcggccgcgt ctcgcggtct tccgcagcaa catccatatc tacgcgcagg tcatcgacga
     8161 cgcctccggg cggacgctgg ccgctgcctc gacgctcgag aaggagctcc gcggcgccaa
     8221 gtcgacgaag accgatgagg cggccgtcgt cggtcgtctc ctcgccgagc gggcgaagac
     8281 cgccggcgtc gagcgcgtcg tgttcgatcg agccgggttc cggtatcacg ggcggatcaa
     8341 gtcgctcgcc gaagccgccc gcgaagccgg cctcgagttc tgatcaagag gagctgacgt
     8401 ggcacggatc gatcccaaca agtacgcgct cgaggagcgc gtcgtccaga tcaaccgggt
     8461 ggcgaaggtc gtcaagggcg gccgccgttt cagcttctcg gcgatcgtcg tcgtgggcga
     8521 cggagccggc acggtcggcg ccggcctggg caaggccggc gaggtccccg aggccatccg
     8581 caagggcgtc gaggacgcca agaagaacct gatccggatc ccgatggtcg ggacgacgat
     8641 cccccacgag gtccgccagg tcttctcggc gtcgcgggtg atgctgaagc ccgcctcgca
     8701 gggcacgggc gtgatcgccg gcggcgcggt ccgggcggtc gtcgaggcgg ccggcatccg
     8761 cgacatcctg gccaagacgc tcggctcgac caacccggtc aacgtgaccc gctgcacgat
     8821 cgaagcactg cgcagcctgc actcggccga ggaactgagc gcccgtcgcg gggtcaagct
     8881 cgtgagccgc atctccggcc agccggccgc ggtggcgacg gaggccggcg atggccgata
     8941 agcttcgggt cacccagatc aagagcacga tcagccacat cgcccgcaac cgggcgacgg
     9001 ttcgcgccct cgggctgaag cggatcgggc acacggtcga gatccccgac aatcccgcga
     9061 ctcgcggcat ggtccgccag gtccgcttcc tcgtctccgt cgaggaggtc ggcggggccg
     9121 acgcccagga gaaggcatga aattgcacga cttgcgtccg ccggacggat cgaacaccgc
     9181 ccggaccagg gtcggacgcg gcatcgcggc cggcaagggc aagactgccg gccgcggcac
     9241 caagggccag aaggctcgtg ccggcggttc gatcccgccg tggttcgagg gtggccagac
     9301 gccgctccac atgcgcatcc cgaagctgcg cggcttcaag aaccgggcaa agatcgacta
     9361 cgaggtcgtc aacgtcggcg acatcgcggc ccggatcgac gccggcgcgt tcgccacggc
     9421 cggcacgacc ccgaagaagg gcgagcagat gacgatcaac gcggacatcc tccgcgcggt
     9481 cggcctcgtc cggaacgtca agaagccgct caagatcctc ggcaacgggg acctcgcgac
     9541 ggccctcttc gtcgtcgccg acgcctacac caagtcggcc accgcgaaga tcgaaggcgc
     9601 cggcggaacc gtcaacgtcc tcgagatccc ggtcgaatcc cggccggcgc tcggtctcga
     9661 cagcgacggg gtcgcgatcg agcgcaccgc ccgcgtgccg cggaccgccg ccggccgcga
     9721 cgccgccgat cgcgaggtcg acgcccgcgc ccgtcgtgat tccgacgcgg ccgaggcaga
     9781 ggctgcagcc actgccgcct cggccgagaa ggccgaacgc ccgaagaagg cgcccaagtc
     9841 cgccgacccg tcgaaggcca aggcggaggc gcccgacgcc ccggccgagg cgtcgacccc
     9901 ggcccccgtg gccgacgccg ccgacgtgac cgatgcgccg gacggcgcgg cctcggccga
     9961 tgagccggcc gacgcacccg atgcggtcgt cgacgacgcg gccgcggcgc ccgacggcgc
    10021 ggtcgatgcg gacggcgatg ccccgctgcc gaccggcgac gaaagcgcgt agcgaccggt
    10081 gttcgagtcg ctcctgaacg ccttccgggc gccggacatc cggcgccgcc tgctctacgt
    10141 ccttgggatc ctcatcgtct tccggttcct ggcccacgtg cccgtcccgg gcgtcaacca
    10201 ggaccagctc gcgtcgtatc tcaaggacaa cgccctgttc ggggtcctcg acctgctgtc
    10261 cggtggcggc ctgtcgagct tctccgtcgt cgcattgggc gtgaatccgt acatcaacgc
    10321 ctcgatcatc atgcagctga tgagcggcgt gatcccgtcg ctccaggcgc tccagcgcga
    10381 gggcgaatac ggccgcaaca agatcaacca gtacacccgc tatctctcgg tgccgatggc
    10441 cctcctccag tcgtacggct tcctcgccct gctcaactcg caggggatcc tgtcgtccgg
    10501 cttcgacctc gccaacggcg cgaccctgac ccagatcgcg accctgaccg ccggtacgat
    10561 gaccctgatg tggctcggtg agctgatcac cgagaagggc atcggcaacg gcatcagctt
    10621 catcatcttc gccggcatcg tcagccgcac cccgggcgcg atcggcggct tccttgcgac
    10681 tccgaacctg cccctgatca tcggcttcac gatcatcgcg atcgtctcgg tggccgtgat
    10741 catctacatc caggaaggcc agcgccggat cccgatccag tacgccagcc gcgtccgcgg
    10801 ccggcggatg taccagggcg gccagacgtt cctgcccctg cgggtcaacc aggcaggcgt
    10861 catcccgatc atcttcgcga tcagcatcct gctcttcccg acccagatcg cctcctactt
    10921 cacgggctcc gaggtcgact gggtgaagtc gatcgcgaac gccatcgtcg gcttcttcaa
    10981 cccgcggacg attccctacg tggccctgta cttcgcgctg accgtcgggt tcacctactt
    11041 ctacacggcg ttcacgttca agccggacga caccgccgag cagctccgca agaacggcgg
    11101 gttcatcccg gggatccggc cgggccgccc gacggcggat tacctcgccc gggtcgtcac
    11161 caggatcacg atcgccggcg ccctgttcct cggaatcgtg gcgaccctgc cgaccctcct
    11221 cgggttgatc ttcccgtcgc tgtcggggat tgccctcggc ggcaccgggc tgctgatcgt
    11281 cgtcagcgtc atcgtcgaga cgatgaagca ggtcgaggcg caactcctga tgcgcaacta
    11341 cgaaggcttc atccgatgac atcgcccatc gagacggcca tccgcgacga acgagcgatc
    11401 cgtatcacgc aatgacggtg gtggttctcc tcggttctcc aggagccggg aaagggacgc
    11461 aggctccggt tctgtcacgc cgtctggggg tcccggtgct cgcgtcgggt gacctcctcc
    11521 ggacggcgct cgccaagggg acggccctcg ggcgcgaggc cgaccgatac atgagccgcg
    11581 gccagctggt gccggacgag acgatcgtgc gggtgttcct cgatcgcctc gagcaacctg
    11641 atgcggccga cggcgcgatc ctcgacgggt tcccgcggac gcggatccag gccgaggcgc
    11701 tcgaccgcgc cctgatcgag gcgggtcgcc gcgtcgaccg ggcgatctac atcgacgttc
    11761 ccaccgagga tctcgtccgg cggatggcga accgccggat ctgcgccgcc aacggccacg
    11821 tctacaacct cgcgtcgaac cctccgctcg ttgccggcat ctgcgacctc gacggctccg
    11881 agctcgtcca gcgggtcgac gacaacgagc tgacggcccg ggccaggatg tccgagcaga
    11941 tcccgccatt gctcgaagtc gtcgaccact atcgcgcggc cggtgtcctc caggtcgtcg
    12001 acggccgaca gcccatcgcc gagctcagcg agaacatcgt tgccgctggc gcaacgcccg
    12061 tccccgatca cgcccccgac tggcagccgg ccggctgatg gtcacccgca agtcccgccg
    12121 cgagatcgag aagatgcggg ccgccgggcg gatcgtggcc gaggtcctcg ccctcgtcga
    12181 atccgagctc cgaccgggcg tctccacggg ccacctcgac cggctggccg agcgccacat
    12241 ccggtcggcc ggtgcggtgc cctccttcat gggctacggc caccgcagca atcccttccc
    12301 ggccagcctg tgcatctcga tcgacgacga ggtcgtccac ggcatccccg gcgaacggct
    12361 gatccgcgac ggtcagatcg tgtcggtcga cgccggcgcg atcttcgacg gctggcatgg
    12421 cgacggcgct cggacattcg tcgtcggcga ggccggctcg gaggtcgagg ccctcgtcga
    12481 cacgactcgc ctcgcgatga tggccggcat cgccgccgcc gtcccgggcg ccaatctcgg
    12541 cgacatctcg ggcgcgatcg aggacgccgc gacgcccggt ggctatggca tcgtccgcca
    12601 gttcgtcggc cacggcatcg ggaccgagat gcaccaggag ccgcagctgc cgaactaccg
    12661 gaccggcgtc cgcggcatga agctggtccc gggcatctgt ctcgccatcg agccgatgct
    12721 gacccttggc ggcccggagg tctcgatcaa ggccgacggc tggacggtcg tcacccgcga
    12781 cggctccctc gccgcccatt tcgagcacac gatcgcggta acggacaacg ggccagagat
    12841 cctgacgacc atttgatgaa ctggcaacct gcgcgccgat ccggccatcg cccagcgaag
    12901 tccgaggctg agctccgcga ggccatcggc ctgcggttgc cgagctcgaa tttgcccggg
    12961 ccggtcatct ttgatacgct acgcgttcgt gtgtcggccc gttcgggccg gcactcatcg
    13021 tgagaaaggg aagcacacaa gcctgtggcc aagcgtgacg cgatcgaggt ggagggaacg
    13081 gtcatcgagc cgcttccgaa cacgatgttc gcggtcgagc tggagaatgg ccaccgggtg
    13141 cttgcccaca tcagcgggaa gctccggatg aatttcatcc ggatcctgcc aggggaccgc
    13201 gtccgggtcg agctgtcgcc ctacgacctg acccgcgggc ggatcaccta ccgcctgaag
    13261 taaccgcgaa ccggttcgcc ggccgccaac gaacacgagt cgaggatccg ttgaaagtca
    13321 gagcttccgt caaggcgcgc tgcgacaact gcaaggtcat ccgtcgccac ggcaccgtga
    13381 tggtcatctg caccaacccg aagcacaagc agcggcaggg gtaacgatgg cgcgcattgc
    13441 cagtgtcgac atcccgcgcg aaaagcgcgt cgagatcgcc ctcacctaca tcttcggtat
    13501 cggcctgccg acggcgcaga agatcctgac ccagacgaac gtgaacccgg acacccgggt
    13561 ccgcgacctc accgaggagc aggtcaaccg gctccgcgag ctcatcgacc gccgctacaa
    13621 ggtggaaggc gatctgcgtc gcgaggttgc gctcaacatc aagcggctga tcgagatcgg
    13681 gtcgtaccgc gggatgcgtc atcgacgcaa cctgccggcg cgcggccagc gaaccaagac
    13741 gaacgcccgt cagcggcgcg gaccgaagaa gacggtcggc gtgcggcgga agaagtaggc
    13801 cgaggtacct gaacgagcat ggccgaacgc aagcgggtag tgaagcaacg ccgcaaggaa
    13861 cggaagaatg tcccccaggg acaggtccac atccaggcga cgttcaacaa cacgatcatc
    13921 acgatcaccg acctgaccgg tgccgtgatc agctggggct cagccggcgt cgccggcttc
    13981 aagggctccc gcaagagcac gccctacgcg gccgcgctct ccgccgacgg cgccgcccgc
    14041 cgggcgatgg agcacgggat gcgccaggtc gaggtctacg tcaagggacc gggtgccggt
    14101 cgcgaacagg cgatccgctc gctccaggcc gccggcctcg aggtcagcgc gatcactgac
    14161 gtcacgccca tcccccacaa cggctgccgc gccccgaagc ggcgccgggt ctgatcgggg
    14221 agggagacca tggctcgcta caccgactcc gtctgccggc tctgccgtcg ggagggcgcc
    14281 aagctctacc tgaagggcag tcgctgctat accaagaagt gcgcgttcga gcgccgtccg
    14341 tcgccccccg gccagcacgg cgtccgtcgc cgcaaggtcg gcgactacgg gctgcaactc
    14401 cgcgagaagc aaaaggtccg ccgcgtctac ggcgtcctcg agcgccagtt ccgcaactac
    14461 ttcgaccgtg ccgagaatcg tccgggcgtg accggcgaga gtctcctccg cacgctcgag
    14521 ctgcgcttcg acaacgtcgt ctaccggatg ggcttcgcca gctcgcgtgc ccaggcgcgc
    14581 cagctcgtcg gccacggcca cttctcggtc aacggcgtcc cgacgaacat cccctcgtat
    14641 cagctcaagg ccggtgaccg gatcgaggtt cgcgagaacc gacgggcgcg tgagcccttc
    14701 aagctggcca aggagaccct ccgctcgcac caggcgccgg agtggctctc gatcgacgcc
    14761 gccaagcttt cggggtccgt cgccgacctg ccccgccgcg accagatgcc gctcgacctg
    14821 aacgagcagc tcgtggtcga gtactactcg aggtaacccg cccacatgat cgaactcgag
    14881 aacccgcaga tcgagcccgt cgaggaagcc gggacgtacg ccaagtacga ggccggaccc
    14941 ctccaggccg gctacggcgt cacgctcggc aacgccctcc gtcgcgtgct gctgtcgtcg
    15001 ctggacggcg cggccgtgac cagcatccag atccgtgacg tctaccagga gttctcgacg
    15061 atcccgggcg tcaaggaaga cgtcacccag atcgtcctga acgtcaagaa gctccgcatg
    15121 aagagcttcg cgacccatcc cgtccagctt cgcctgatca agagcggcgc cggccaggtg
    15181 accgcggccg acatcgccga atcggccgat gtcgagatca tcaaccccga gctcgtgctg
    15241 atgacgctcg ataccgacga cgtcacgatc gagatggacc tcaccgtcga gcgcggcgtc
    15301 gggtacctcg gcgccgagcg gagtgagcag ctcccgatcg gggtcatcgc gatcgatgcg
    15361 atcttcaccc cggtccgcaa ggtcaactac tgggtcgagg gcatgcgggt cggtcagatg
    15421 accaactacg acaagctcac catcgagatc gagaccgacg gcgcgatcac gcccgaggag
    15481 gctctcagcc gctcggccga gatcctcgtc aaccagttcc gcccgttcgt gacgatcggg
    15541 atggccgccg cggtcggtga gcaggccacg ccgggcctgg cccagctgcc cccgaacatg
    15601 ctcgacatgc cgatcgagga gctggacctg ccgatgcggg cctacaactc cctcaagcgg
    15661 aacaacatcg tcaaggtcgg ccagctgctc cagctcaagg acgatgacct tctccggatg
    15721 cgcaacttcg gcaagaagtc gctcgacgag atgaaggaac gcctccggat gcgcggcttc
    15781 atcatccccg agaccgagtc ggatggcctc gaggacgccg ccttcgagga cgaaggcgac
    15841 gacgagggcg agagctgaga tggcccactg catcggcgga cgcaagctca gccgcaagca
    15901 gggcccgcgg ctggctctct tcaagaacct gaccgtgtcg atcctgcgct acgagcgggt
    15961 ccagacgacc gaggcgaagg ccaaggagat ccagggtcgg gtcgagaaga tgatcacgct
    16021 cgccaagcgg aacgacctga cggcccgccg ggccgtcgtg gccgcgttcc cgaacgaacc
    16081 gctcgtcgtg accaagctgt tcgatgagat cgccccgaag tacgcggatc ggacgtccgg
    16141 ctacacccgg atcgtgaaga tcggcctgcg ccgtggtgat gcggctccga tcgtccaaat
    16201 cgaattggtc taggtcctcg agaggggacc gccggtgcgc taccgcgcaa cggttgaata
    16261 cgacggcacg gacttcgccg gcttccaggc ccaacggccg gggatccgga cggtccaggg
    16321 ggaactcgaa gccgcgctgg ctcgcctcgg agacggggtt cgccagccgg tcgacggggc
    16381 cggccgcacg gacgccggag tgcatgcgct ggggcaggtg atcgcgttca ccttcaccgg
    16441 tcgcctgacc gctccgaggc tcgaggcggc actcaatggc cagctcccgc cggacatcgc
    16501 agttcgcgac gtccgggcgg tggcaccgga gttccatcca cgtcgcgcgg cgcggtaccg
    16561 ggaataccgc tacaccgtct ggaacgggcc gcgcagtccg cttcacgagc gtcatgcgct
    16621 cgggatccgg gccccgctcg acatcgccgc gatggcacga gccgggcagg ccttcatcgg
    16681 cccgcacgac ttcagcgcct tcggggtggt gatcgggggc cgctcgccgg tccggaacgt
    16741 gcattcggtc cggatcggga ggaagggaca gctcgtgacg atcgacgtcc gggccaacgc
    16801 cttcctccgg ggaatggtcc gccggatggt ggccgtcctc atcgaggttg ggcacggcaa
    16861 gatgaccgag acagaggtcc gggaggcaat cgccgccagg acgccggctc gcaatggggt
    16921 atccgccccg gctcgcgggc tctgcctccg gcgcgtcgtc ctcggacggc ggctggagat
    16981 ggaaacgaca gacggagacg actgaggaac gatgagcacg aagacctaca cggtccgcgc
    17041 aagcgagatc gagcggcgct ggtacgtcgt cgacgcggcg gacgagacgc tcggccgcct
    17101 cgcgtcgcgc atcgcgcgcg tgctcgaagg caagcacaag ccgacctgga cgccgaacct
    17161 cgattcgggc gaccacgtca tcgtcctcaa cgccggacgg atcaccgtca ccagcaacaa
    17221 gctcgagtcg aagctctaca tccgccacag cggccacccc cagggctaca aggaggagac
    17281 cctcggtcac cttctcgagc gtcgtccgga agaggtcatc cggcgtgccg tcaaggggat
    17341 gctcccgcgc aatcggctcg gcgtccagca gctccggaag ctgaaggtct acgccggcac
    17401 cgatcacccg catcaggccc agcggcctga gccgctcgcc cgatgaggag ccctcgatga
    17461 cagttccttc cttcttctcc ggaaccggtc gccgcaagac ggcgatcgcc cgggtccgcc
    17521 tgctccccgg ggagggcgag atcgtcgtca acggccgttc gctggaggag cacttcggca
    17581 acgccgtgaa cgagtccgac ctccggatgc ccttccgggt gaccgggacc gagggtcact
    17641 acaacgcgat gatcaaggtc gagggcggcg gcgtgaccgg ccaggccggc gcgatccgcc
    17701 acggcatcgc ccgggccctg ctcgaggtcg acgaagccac caaccggttg ccgctgcgcc
    17761 aggccggcct cctcacccgc gacccgcgga tgaaggagcg caagaagtac gggctgaagc
    17821 gagcgcgcaa ggcgccgcag tacaccaagc gctaacggtc gccggcatgg gcggctaagc
    17881 gcggcgttgt gggctgcgcg cgctcactca cgcacctgcg ggtgcgctcg agcgcgtgct
    17941 cgcctccgcc ttgcgctgaa tcaaccctgc gccgtcctcc gctgcgtgct ggactccacc
    18001 gtcagtccgg ccgaccttcg atccgtgcca cccgctagca tggcggcgtg agcaccaccg
    18061 cccccgacac cagcctgcgc gggcgcgacc tgctgtcggt cgcggacctg tcgacggccg
    18121 aggtcgggcg gatcttcgat cgggcggtcg cgctcaaggc ggaggatcgc gcgcgccggg
    18181 cgaccggtgt cgccgcggcc acgccgctga ccggactgac gctggcgatg ctgttccaga
    18241 agccgagcct ccggacccgg gtcacgttcg aggcagggat gaaccagctc ggcggccatg
    18301 cgatctacct gcccgaggac acggtgatgg gcgcccgcga gagcgtccgg gacgtcgccc
    18361 gcaacctcga gcggttcgtc gacgcgatcg tcgcccggac gggcccgcac gaggtcgttg
    18421 tcgagctggc ggcccaggcg tcgatcccgg tgatcaacgc cctcaccctc cgcgagcacc
    18481 cctgccaggc gctggccgac ctcttcacga tgcgcgagcg cttcggcgac ctccgcggcc
    18541 gcgtgctcgc gttcgtcggt gacggcaaca acgtctacca ctccctcgcc ctgctcgggg
    18601 cgatcctcgg gctcgaggtt cgtctcgccc atcctgccga ctacggtccc aacccgcgga
    18661 tcgtcgaacg ggccatcgag ctcgcggccg cggccggcgg ccgcctcgtg ttcgacgacg
    18721 acccgacgac gatcgtccgt ggggcggatg tcgtctacac cgacgcctgg acctcgatgg
    18781 gccaggaggc cgagacggag gcgcgccgcg acgcgttcgc ccgttaccgg gtcgacgagg
    18841 cgctgcttga cgcggccgga cccgacacag cggtgatgca ctgtctcccg gcccatcgtg
    18901 gcgaggagat cacctccgcg gtcatggacg gcccgcggag catcatctgg gaccagtcgg
    18961 agaaccgtct tcatgtccag aaggcgttgc tggtggaggt cctcgccgga tgagcggctc
    19021 tcggcgcggc tccggcaagc gcgcgccgcg tccgggcggg gcgacggcca acgggtcgct
    19081 gtacgagcgg tacaaggatg ccctccgacg cggccacgtc gccgcgctcc ggggacggaa
    19141 cgacgccgca gtcgatgcct acggcgaggc cgcctcgatc gcgccggacc gcgccctgcc
    19201 gcacacgagc atcggcggga tcctcgtcaa gatgaaccga ctcgacgagg cggttgtggc
    19261 ctacgagaga gcgctcgcca tggcgccgcg cgacgagggc gccctccgcg gtctcgccga
    19321 cgttcacgtc cggacgggtc gacggacgga ggcggccgcc acgctggacc gcctcgccga
    19381 cagtctcgat gccgccggac gactggccga tgccaccgat gcggcccgac gagctctcga
    19441 gctggccgaa tcgcgggcga ggcgccgaca ggtcgaaggg ttcgcggggc gcctccggac
    19501 gtcgtcgagc ggcgacgaga ccgcccagcg tgccctcgat caggcgctcc ggctgctgga
    19561 gccgcccagt cgcgcggttg atgccgtcga ggacgacgcc ctcctcgtcg atggcgccac
    19621 cgatgacgac cggccggaca ccgaggcgag gcccgcggcc gaagaggaga ccgccgaggt
    19681 cgtggaggag cccacagccg aggccgctcc cgtcgccgag ccgacaggcg agccggagcc
    19741 cgagcccgag ccagagccgg agcccgagcc ggagatcgtc gccgaacctg aacccgaacc
    19801 cgaaccggcc gaaccagagc ccgaacccga gccagagccg gtgaccgagc cggccggtct
    19861 cgggatcgcg ctcggcgccg ccgccgaggc cgccctccac gccggcgact tcgccgccgc
    19921 gcatgacgga ctcctggccg ccgctcgggc ccatcgccga gccggtcggc tgtcggcggc
    19981 ggtcgacgcg tgctacctgg cgatcgggct ggcgccgtcg gatcccgatc tccacctcct
    20041 gctggccgag ctctacctcg atcgcggctg gcgtggtcag gcgatcgaga agctgctgct
    20101 cctcgggcgg ctggcggacc tcgacgacga cgcagcggtc cggcaacgac tgtgcgacct
    20161 cgtcacggca cggctcgccg acgagccgcg tgtggcggac ctgtgcgcct gatcgccccg
    20221 cccgaccgac ccggcgcgga acgcctcgcc cgcgctgcca cgctcgcgcg acatccgcgc
    20281 cgcgcgagcc cgccccgatc cggccgcggt gggccgatgt agactgcacg gacgatgccg
    20341 cagcttctcg cgtcgatcgt cgatcagatc gacctgacat cgctggtcga catcggaatc
    20401 acggcgctcc tgatctactg gctgttcagc ctgatccgtg gaacgcgggc cgtgagcctc
    20461 gtcatcggcg tcagcgtcct cttcgccgtc tacgccgcgg cccgggcgtt cgatctgcgc
    20521 ctcctgaccc agatcctcca gaccctggcg gtcgtcgggc tgttcgccct cgtcgtcgtg
    20581 ttccagccgg agctgcgccg agcgctcgag cggatcggcc gcgtcgggtc gttcaactgg
    20641 ctcttctcgc cgacggagca gcgggccgtc gagcatgtcg ccgacgaggt cgcccgggcg
    20701 gcggcgctcc tgtcggccga cggccacggc gccctcatcg tgctcgagcg cgacaccggg
    20761 ctcgaggagg tcgccgagac cggggtcatg ctccatgccg acatcagcgt cgacctcctg
    20821 cgcacgatct tcgccccgcg gaccccgctc cacgacggcg cggtgatcat ccgcgaggag
    20881 acaatcctcg ccgccgccgc cctcctgccg ctggccgaga tgaccatcca caccgaacgc
    20941 ttcgggaccc gccaccgggc cgccctcggg atcaccgagc agaccgacgc cgtcgtcgtc
    21001 gtcgtgtccg aggagaacag ccaggtcagc ctcgtcgagc gagcgcggat cgtgcgaaac
    21061 ctgagcgagc cccagctggc caaggcgatc aaggcgctcc tcgccccgac cgacggccgc
    21121 ggccggctcg gtcggcgggt cctgccggat gcccagaccg gcggtcgcgc cccgcgcctg
    21181 tccgacctcg gtcgtttcgt cggacgtggc cggccgccgg tcgtctcgcc gccaccccga
    21241 gccgacgacg acccggcacc cgcacgagcg ccccgatgac ccgtctcctc ggggcgatcg
    21301 tctacaactg gcctctcaag ctgatggcga tcgtcctcgc cacgctcctg tatgccggcc
    21361 tcgtggtctc gcagtcgacg ttcgagatcc cggcggccct acagatccac ccgctcaacc
    21421 aaccgctcac cgccgtcatc ctcggcaacc tgccgtacgt cagccgggtc cgctacgtgg
    21481 ccaacggcga tgtcggggcc ggtccgacgc cggacagctt ccgcgcgacg atcgatctca
    21541 gcggcgtcaa tccggaggcc ggctcgacct acgtcaagat cgacgtctcg tcgaacgacc
    21601 cgcgcttcct cgccgtcgac tgggagccgc gcgggatcaa cgtccaactc gacccgttca
    21661 ggtcatacca ggtcccggtc cgggtgaaca agggcaccgt gccggacaat ctcgatgtcc
    21721 gcccggccga gctcagcgtc tcgaccgtca cggtatccgg gccggactcg gtggtgaagt
    21781 tcgtcgtcga agcccgggcg gacgtgctca tcgacccgtc gggcaccccc gtcgaccgcg
    21841 acgtgccgct catcccggtc gatcagctcg gcaaccgcct caccccggtc caggtcgaac
    21901 cggccagcgt ccacgtcacc atcgcggtct tcaacaactc gcgcaagaaa gtgctggcgg
    21961 tcaacccgat cgtgaccggg acgccgccgc aggggtacgt cgtcgacacg atcaccgtga
    22021 ccccgtcaac cgtcaccgtc gagggcaacg ccgaccagct gtcggccctc gtccgggcgg
    22081 acacaaaggc gatctcgatc gggaccgcga cgggcacgat cgacacggat gtcgacctgg
    22141 acctgcccgc cggagtgctg ccgatcggct cgaaatccgt ccacctgacg atcacgctca
    22201 aggtggagac cggcacgaag gccttcgccg ccgccctcgt cccggtcggg cgtcaacccg
    22261 ggctcgagta ccggttctcc acgctcaaca tcctggtcaa cgtcgccggg ccgctggccg
    22321 acctcgatcg gctcgacccg tcgagcttca ccgtgccgct cgacgtcacg ggtctcgggg
    22381 tgggcgtcca cgaggtcatg ccgacgccga acctccaggc cgggctgcgc ctcctgaacg
    22441 tcgacccggc gacggtcacc gtcacgatca ccgcgcccgc cagcccggcg ccgtcggcgg
    22501 gcggctgacc gtgccccgcc tgttcgggac cgacgggatc cgcggcgtcg ccaacgtcga
    22561 gctcaagccg acccttgcct acgccctcgg tcgggcgacc gcctaccagc tcgtcgggca
    22621 gggcggccgg ctcgtcgtcg gccaggacac ccgtcggtcc ggcgacatgt tcgtgtccgc
    22681 gatcacggcc ggcgcaacga gcctcggcgc ggacgtccac atcgcgggct gcgtcccgac
    22741 cccggccctc gcgttcctcg ccggaaccgg cgacttcgcg gccgggatca tggtctccgc
    22801 gtcgcacaac ccggccgacg acaacgggct caaggtcctc gactcgaagg gcctcaagct
    22861 cgacgacggc atcgaggagg agatcgagca gctgatctgg cggaccgagg agctcggctc
    22921 ggtcggcaac gccgagatcg gccgggcgat cgttgacccc gggctcctcg accgttaccg
    22981 cgagcatcgt ctcggcctgg ccggttcgat ctcggccgtc ggcctccgga tcgtgctcga
    23041 ctgcgcgaac gggtcgggcg gcgtcgtcgg gccggagatc ctggccgcga ccggggcgag
    23101 cgtcgaggtc atccacaacg agcccgacgg gatcaacatc aacgtcgcgt cgggggccac
    23161 gtcgccgtgg tcgctcgcgg cggcggtcaa ggagcggggc gccgacgtcg ggttcgccct
    23221 cgacggcgac gccgaccgcc tgatcgcggt cgacgcgacc ggcacgatcg tcgacggcga
    23281 ccaggtcctc ggcatcctcg ccctcgaccg cctcgatcgc ggcgtactcc cggagggcgc
    23341 cctcgtcgtg tcggtcctct cgaacggcgg tctccaggcg gcggtcgagg cggccggcgg
    23401 ccaggtcatc cggactcccg tcggggacaa gtacatcctc gaggggatgc aggtgtcggg
    23461 ggccggcctc ggcggcgaga agagcggcca cgtcatcgtg ctcgagcaca cgacctccgg
    23521 tgacgggatc gtgaccgcgc tcgaggtcct ccgggtgatg accagggccg gtcgcccgct
    23581 cgccgagctg gccgagcgga tccagatgct gccccagcag caacgggcgg tccgggctcg
    23641 ccaccgggat cactgggaag gggatgccgc gatccagcgg gcgatccggg ccgccgagga
    23701 gcggctcggc gctggtggcc gggtgctcgt ccgaccgtcg ggcaccgagc ctgcgctgcg
    23761 ggtgatgatc gaaggccccg acgccgacct ggtcgccgag ctggccgact cgatcgcgac
    23821 cctcgcggga gagcgtctaa actga
//
LOCUS       DATAIW010000166         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212201, whole genome shotgun sequence.
ACCESSION   DATAIW010000166 DATAIW010000000
VERSION     DATAIW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212201"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHM48_05390"
     CDS             <1..311
                     /locus_tag="VHM48_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2754873.1"
                     /translation="ILQIVGVTDPLKLGFQQQAMIGVRCEASQLLEAAEVINAFPDVD
                     YLVVTAGSYDLLVEVVCDDNEALLHFLADKLRRVAGVRETGTFVYLRMLKQVYTWGTR
                     "
     gene            complement(328..1518)
                     /locus_tag="VHM48_05395"
     CDS             complement(328..1518)
                     /locus_tag="VHM48_05395"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2754874.1"
                     /translation="MTTIGIDIGGRTHVVARCRDGTSRADREILRVNQSRAGFAALDA
                     WLGRQAEPVTLVTMESSGHYWMPLASHLCRRDIPVAVVNPLAAKYFAKSRLARTKSDP
                     ADARSLAEMAMRDTPPARDPLTGVELRQAARFAMTLVAEQAKVCQRLIRLVELGFPEL
                     GELFDDPTCRTAREILRIAPTARAATRRRTSTLANANAGPGRRRLGTTKAERLQTAAV
                     DSIAVPELDAEVAFEVGLLLDQYDLLERQIEEADRRVAGLLDGETARRLQTIPGVGPS
                     IAAALLAEIGDIDRFTDFDQLLAYAGVHPAERSSGRKGANPETAWHMSKAGNAHLRAA
                     AYRMALVGIQHNPVIAAHYARKRAAGKSKMNALGHCMRKALSIVWGVWRNGRDFDPNW
                     GVEA"
     gene            complement(2134..2325)
                     /locus_tag="VHM48_05400"
     CDS             complement(2134..2325)
                     /locus_tag="VHM48_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754875.1"
                     /translation="MHQYRGHVVNYSVVGNAQQKIGSYDVDCLDAWMDQGVPWDNNGD
                     ESNEANSINAEAVCLGANQ"
BASE COUNT          401 a          897 c          904 g          430 t
ORIGIN      
        1 gcatcctcca gatcgtcggc gtcaccgacc cgctcaagct cggcttccag cagcaggcga
       61 tgatcggggt gcgctgcgag gcgtcccagc tcctggaggc tgccgaggtc atcaacgcct
      121 tccccgatgt cgactacctg gtcgtcaccg ccgggtcgta cgacctgctg gtcgaagtgg
      181 tctgcgacga caacgaggcg ctcctccact tcctggcgga caagcttcgc cgcgtggccg
      241 gcgtccggga gacggggaca ttcgtctacc tgcggatgct caagcaggtc tacacgtggg
      301 gaacgcgcta gatcccatag ggtcttgtca agcttcgacc ccccaattgg ggtcgaagtc
      361 ccggccgttg cgccagacgc cccagacgat gctgagcgcc ttgcgcatgc agtggccgag
      421 cgcgttcatc ttggacttgc cggcggcccg cttccgggcg tagtgggcgg cgatgaccgg
      481 gttgtgctgg atgccgacga gggccatccg gtaggccgcc gctcggagat gagcgttgcc
      541 ggccttgctc atgtgccaag cagtctcagg gttcgccccc ttgcgcccgg agctccgctc
      601 ggccgggtgg acgccggcat aggcgaggag ctggtcgaag tcggtgaagc ggtcgatgtc
      661 gccgatctcg gcgaggagcg ccgccgcgat cgacgggccg acgcctggga tcgtctggag
      721 gcgacgggcg gtctcgccgt cgaggagacc cgcgacgcgt cggtcggctt cctcgatctg
      781 gcgctcgagc agatcgtact ggtcgagcag gagccccacc tcgaaggcaa cctccgcgtc
      841 gagctcgggc acggcgatcg agtcaaccgc ggcggtctgg agccgttcgg ccttcgtcgt
      901 ccccagccga cgacgaccgg gcccggcgtt ggcgttggcc agggtcgacg tccgccgccg
      961 ggtcgcggcc cgcgccgtcg gcgcgatccg gagtatctcc cgagcggtcc ggcaggttgg
     1021 gtcgtcgaac aactccccga gttcggggaa gccgagctcg acgagccgga tgaggcgctg
     1081 gcagaccttc gcctgctcgg ccaccagggt catcgcgaag cgggcggcct gacgcaactc
     1141 gaccccggtg agcgggtcgc gagcgggtgg cgtgtcgcgc atggccatct cggcaaggct
     1201 gcgggcatcg gccggatcgg acttcgtccg ggcgagccgg ctcttggcaa agtacttcgc
     1261 cgcgagcggg ttgaccacgg cgaccgggat gtcgcgccgg cacaggtgcg aggcgagcgg
     1321 catccagtag tggcccgacg actccatcgt cacgagcgtc actggctcgg cctgtcgacc
     1381 gagccaggcg tcgagcgcgg cgaacccggc ccggctctgg ttcacccgga ggatctcccg
     1441 gtcagcgcga ctcgtgccat cccggcagcg ggcgaccaca tgcgtccgtc cgccgatgtc
     1501 gatcccgatc gtcgtcatcc ggacagcctc cttccgcaga ccccaggggt gcccgcccgc
     1561 ccggtcgacg tcagctgatg ggtccgatca ccggtcagag ccgcaggatc ccgatcaccg
     1621 ttcacggccg agggtcgggg gccggacact agcgccaacg gtcatctgcc ggatagcgag
     1681 gtgccggtct ccccgtggag gtcgctgcca tcatcgacct cccacaaggg ggtatatcag
     1741 cgaacacacg gacgtggtca ccttcgggcg ccgctatgtg gaccgtcggc cagaggccga
     1801 cgtcgaccga cctgatcgac gtcgggtcca cgcccggcgg ggtcgcggat gaccacgtca
     1861 tcccgcggtc cgtcgacacc tcgactccat gactcgtgat tgcccagccg atcccgtttg
     1921 gagcgaggcc agcgtcgagc aggcccgtgc caaccgcggc cgtcgtcgga atgctgcccg
     1981 tcggcgtcgg gggcggggca accgtcgccg ctgcggaggc ttcaggactc ccactcgggc
     2041 cgggcaccgg cgcgggcgcg ccgacgcacg cgatgaccac gaacagggac gagatggcga
     2101 caatcaggga ttgtcgaggc gagatggatc ggttcattga ttcgccccca gacacacggc
     2161 ctcggcgttg atcgagttgg cttcgttcga ctcgtcgcca ttgttgtccc atgggactcc
     2221 ttgatccatc catgcgtcga ggcagtcgac atcgtagctg ccgatcttct gttgggcgtt
     2281 gcccacaacg ctgtagttga cgacatgacc tcgatactga tgcactcgtt ggtccatggg
     2341 ccagttcccg ttgggaacac ccgggagatt ccagacagtg ttcggattgg cggccgggga
     2401 gccccacgcc gcgagccaca cctctgccgg cggatgggcg atcgtcgccc agtcatccgc
     2461 gaaggaaccg tacgaggatc catagacgcc cagtcagctc cgagtgtgct cgaccagccg
     2521 tcaacgtacg cctttaccgc ggccctcgct gtcgcgagag ccgttccggt gcaccccggg
     2581 gcgccagagc ccatgcccat tgcatctgcg cggccgtcgg gacggcacag ct
//
LOCUS       DATAIW010000167         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_182664, whole genome shotgun sequence.
ACCESSION   DATAIW010000167 DATAIW010000000
VERSION     DATAIW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182664"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1673)
                     /locus_tag="VHM48_05405"
     CDS             complement(<1..>1673)
                     /locus_tag="VHM48_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX2754876.1"
                     /translation="AGARLADLYPDLRAPGIRVRYALFHQRYATNTQPTWRLAQPFHA
                     IAHNGEINTVRGNRSQVRGRAGDPAGVAAAELAAAGPLVSDAGSDSQSLDELLELLVS
                     TGWDLGTALLTAMPEAQALRRAPHPQVAALRRRTAGFIAPWDGPAAIVFGDGRRVGAI
                     ADRNGLRPTAFAVTRDRLVAVCSEAGAIPLPPEETVRRGRLGPGELFLVDPARGLVLE
                     DVEAKAHLLRRLPIHDAPRVAHVDPIVGDDDSSIVLDGTPTGADLRFLAGLHAENARL
                     DLKTMALEGHEPLWSMGDDTPIPGLGRIDRPVADHLRQSFAQVTNPPIDPERERTVMD
                     LRVELGRRPALLGGPPAGSRTIRLERPFLADLAALLRAFAGPVRHLDATWSAADGAAG
                     LAAALDRLATDAVVAARGRTELLVLSDRGFSLDRLPIPSVLAAGTVDAALSAAGLRGR
                     ADVVVEAADVLDVHGAAMVLAVGATGLVPWLAVEMAAELAGTRGAEELDAAATVGNLL
                     AAFDAGLRKTLARMGISTASSYIGGKLFETIELDADLRERCFPAAPAWP"
BASE COUNT          229 a          609 c          651 g          184 t
ORIGIN      
        1 gggccacgcc ggcgccgccg ggaagcagcg ctcacgaagg tcggcgtcga gctcgatcgt
       61 ctcgaacagc ttgccgccga tgtacgagga cgcggtgctg atccccatcc gggcgagggt
      121 cttccgcaac ccggcatcga atgcggccag gaggttgccg acggtcgccg cggcatcgag
      181 ctcctcggcg ccgcgcgtcc cggccagctc ggcggccatc tcgaccgcca gccatggcac
      241 gaggcccgtc gccccgaccg cgaggaccat cgccgcacca tggacgtcga ggacgtcggc
      301 cgcctcgacg acgacgtcgg cccggccgcg cagcccggcc gcgctgagcg ccgcgtcgac
      361 cgtcccggcc gccagcacgg acgggatcgg cagccggtcc agcgagaacc cgcgatcgct
      421 caggacgagc agttccgtcc gaccgcgggc ggcgacgacg gcgtcggtcg cgagacgatc
      481 cagcgcggcg gcgagcccgg cagcgccgtc ggcggccgac caggtcgcgt cgagatggcg
      541 gaccgggccg gcgaatgccc ggagcagcgc cgcgaggtcg gcaaggaacg gacgctccag
      601 acggatcgtt cgcgaaccgg cgggcggccc gccgaggaga gccggccgcc ggcccaactc
      661 gacgcgcagg tccatgaccg tccgctcccg ctccgggtcg atcggcgggt tcgtgacctg
      721 ggcgaacgac tggcggagat ggtcggcgac cggccggtcg atccggccga gccccgggat
      781 cggcgtgtca tcgcccatgc tccagagcgg ctcgtggccc tcgagggcca tcgtcttgag
      841 atcgagccgg gcgttctcgg cgtggaggcc ggccaggaac cgcaggtccg cgccggtcgg
      901 cgtcccgtcg aggacgatcg acgagtcgtc gtcgccgacg atcgggtcga cgtgggcgac
      961 ccgcggggcg tcgtggatcg gcaggcgacg gaggagatgg gccttggcct cgacgtcctc
     1021 gaggacgagc ccgcgcgccg gatcgacgag gaacagctcg cccggcccga gccggccgcg
     1081 tcgaacggtc tcctcgggcg gcagcgggat cgccccggcc tcggagcaga ccgcaacgag
     1141 ccggtcgcgg gtcactgcga aggccgtcgg gcgcagcccg ttccgatcgg cgatcgcccc
     1201 gacccggcgc ccgtcaccga acacgatggc cgccggaccg tcccacggtg cgatgaaccc
     1261 ggcggtgcgc cggcggaggg ccgcgacctg cggatggggg gcacggcgga gcgcctgcgc
     1321 ctcgggcatc gcggtgagga gcgcggtccc gaggtcccag cccgtcgaga cgagcagctc
     1381 gaggagctcg tcgagcgact gcgagtccga gcccgcgtcg ctgacgagcg gcccggccgc
     1441 cgcgagctcc gcggccgcga ccccggccgg atcgccagcc cgcccgcgga cctgcgagcg
     1501 gttgccacgg accgtgttga tctcgccgtt gtgggcgatc gcgtggaacg gctgggccag
     1561 ccgccaggtc ggctgggtgt tggtcgcgta gcgctggtgg aacagggcgt agcggacccg
     1621 gatcccgggc gcccgcaggt ccgggtagag gtccgccagg cgggcgccgg cga
//
LOCUS       DATAIW010000168         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_97988, whole genome shotgun sequence.
ACCESSION   DATAIW010000168 DATAIW010000000
VERSION     DATAIW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97988"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VHM48_05410"
     CDS             <1..923
                     /locus_tag="VHM48_05410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX2754877.1"
                     /translation="IVVAGESLVDLLVRPDGGVTATPGGGPFNVARALGRLGLPVAYL
                     GRLSTDPFGRLLRARLVADGVDLGLTPTTDDPTLLAVVEIDDDGTATYRFHAIGSAAV
                     GLGPRDVANGLPVETTALHIGTLGLVLEPMASTIAGLVATAPPGVLVMADPNCRPSAI
                     ADPAAYRSRLATLMRRVDVVKLSADDLAWLDPERDVVDASRAILESGPAVVLLTDGGH
                     PTRIVTRRHVVEVAVPEVTVVDTVGAGDAFGAGFLAAWTRAGRGRLELEDLDAVNDAT
                     RFAIEVGAVTVGRAGADPPTLAELGASAQT"
     gene            961..1983
                     /locus_tag="VHM48_05415"
     CDS             961..1983
                     /locus_tag="VHM48_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX2754878.1"
                     /translation="MSGSILASPPIRRAARIGSVAGLVLVALVATAGPAVAASGVAWP
                     TQSRGDRGTDVLTIQHLLRGRLAAEPVPAVALPPADGQFRSSTDAAVRAFQSAHGLAP
                     DGVVAWATWGRLIQRLGRGASGEAVRALQVELVEKVGAAISVSGSFGAATEAALITFQ
                     RHMGLPRTGVVDTATWRALAWHFELPRFAATSLCDYSAGNGPANWGTAEAIATTEAVG
                     RTVFALGFGRVAVGDVSFEHGGPLPGHDTHRFGLDVDVRPLRWANDQCAVAGTTWRST
                     AYDRAATRTMILKFRVYAVGHIELIYFNDPILIGEGLTTWHTGHDDHVHVRFCETTHP
                     LSTYDC"
     gene            complement(1999..3774)
                     /locus_tag="VHM48_05420"
     CDS             complement(1999..3774)
                     /locus_tag="VHM48_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754879.1"
                     /translation="MPARPLLRSLADRFGGWTPALLGLGLIVAFLVSRLPLLDADIPQ
                     WELTVYSPIDEFTYSLPAFNLVHYGTWLHQAASWAPLEGQPTNVLQNVVVALTLKLFG
                     STFWGLRMSSILFGLVGFLSLVSIVRIQADEARRFDAVPKRLAWLVVAAACVLLLVDF
                     SSLLSARIVEPTISRIAVATLLIALVARGVFLGERHGLVRSGVFGATAAAAVSFVYIY
                     NAFLVPGALVALVWWAYRRGGWAAVGRHTIAFLVGCVVAAGLYYGLIYLVYGHSPIDW
                     YRAWISPLATSSRGNGLSLTKIASILEANIFRLDPAFVGVVLASIPVFAWTLLRRPNA
                     WMVLIAASLAAFLAQTSFVADYPERKFIMVMIFALPVAAAGVFGWRRFQAWVTADHRR
                     LVAATVWLTGAVFVAAWASPFGKVPPHGSILARIVLVAGLVGAAALVALLIVRRPRFT
                     TLAALVLAAAILVPLAYADLAFVYRRPTFTYRDAQIAATRDVGDQETAGSLSFGMQLY
                     NGSRSVLNGVTVPQAEYEADIVRFFREGGATSMYAYADPKSRTRYESLGFRLVETLPI
                     LLPRGMKLGRYVFGASGAAVSDDRG"
     gene            3892..>4262
                     /locus_tag="VHM48_05425"
     CDS             3892..>4262
                     /locus_tag="VHM48_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2754880.1"
                     /translation="MATRTPANPPVEPRSTIGSGAVSRVERAPTSWGLGDGAAIPWEY
                     APAPESRDVVTLKERYGLFIGGKDVAASDGETFVTVNPATEEPLAVVARATAADMDRA
                     VRAARSAFRRSWSTLSGRERA"
BASE COUNT          646 a         1492 c         1563 g          561 t
ORIGIN      
        1 tgatcgtcgt cgccggcgag agtctcgtcg acctcctcgt ccggcccgat ggtggcgtca
       61 ccgccacccc cggtggaggc ccgttcaatg tcgcccgggc cctcggccgt ctcggtctgc
      121 ccgtcgcgta cctcggccgg ctctcgacgg acccgttcgg gcgcctcctc cgagcccggc
      181 tcgtcgcgga cggggtcgat ctcggcctga cgccgacgac cgacgacccg accctcctcg
      241 ccgtcgtcga gatcgacgac gacgggacgg cgacctaccg gttccacgcg atcgggagcg
      301 cggccgtcgg cctgggtccg cgcgacgtgg cgaacggcct gccggtggag acgaccgcgc
      361 tccacatcgg aacgctcggg ctggtcctcg agccgatggc ctcgacgatc gcggggctcg
      421 tcgcgaccgc cccgcccggc gtgctcgtga tggccgaccc gaattgccgg ccgtccgcga
      481 tcgccgaccc cgccgcctat cgcagccgcc tggcgaccct catgcgccgg gtcgacgtcg
      541 tcaagctcag tgcggacgac ctggcgtggc tcgatccgga gcgtgacgtg gtcgacgcct
      601 cgcgcgcgat cctcgagtcg ggcccggccg tcgtcctgct gaccgacggc ggccacccga
      661 cccggatcgt gacgcggcgg catgtcgtcg aggtcgccgt gccggaagtg accgtcgtcg
      721 acaccgtcgg cgcgggcgat gcgttcgggg ccgggttcct cgcggcgtgg acgcgcgcgg
      781 gccggggccg gctggagctc gaagacctgg acgcggtcaa cgatgcgacc cggttcgcga
      841 tcgaggtcgg cgccgtgacg gtgggcaggg ccggagccga cccgccgacc ctggccgagc
      901 tgggcgcgtc cgcgcagacc tgattggcgc gtcggcgtcg ggctgatacg ctccgggccc
      961 gtgtccggat cgatcctcgc cagcccgccc attcgccgag ccgcccggat cggcagcgtg
     1021 gcgggcctcg tcctcgtcgc gctcgtcgcg accgccggac cagccgttgc cgcctccggg
     1081 gtggcctggc cgacccagag ccgcggcgat cgtggcaccg acgtcctcac gatccagcat
     1141 ctgctccgtg gccggctcgc cgccgaaccg gtgccggctg tcgcccttcc gccggccgat
     1201 ggccagttcc ggtcctcgac cgacgccgcc gttcgcgcgt tccagtcggc ccacggcctc
     1261 gccccggacg gcgtcgtcgc ctgggcgacg tggggcaggc tgatccagcg tctcgggcgc
     1321 ggtgcgagcg gggaagcggt ccgggcgctc caggtcgaac tcgtcgagaa ggtcggggcc
     1381 gccatttcgg tcagcggctc gttcggggcc gcgaccgagg ccgcattgat caccttccag
     1441 cgccacatgg gcctgcctcg gacgggcgtc gtcgacacgg cgacgtggcg ggcgttggcc
     1501 tggcatttcg agctgccgcg gttcgccgcg accagcctgt gcgactactc ggccggcaac
     1561 ggcccggcga actgggggac cgccgaggcg atcgcgacga ccgaggccgt cggccggacc
     1621 gtcttcgccc tcgggttcgg gcgggtggcc gtcggcgacg tgtcgttcga gcacggcgga
     1681 ccactccccg gccacgacac ccatcggttc gggctcgacg tcgatgtccg gccgctgcgc
     1741 tgggcaaacg accagtgcgc ggtggcgggg acgacatggc ggtcgacagc ctacgaccgg
     1801 gccgcgaccc ggacgatgat cctgaagttc cgggtatatg ccgtcggtca catcgagctc
     1861 atctacttca acgatccgat cctgatcggc gaggggctca cgacgtggca cacgggccac
     1921 gacgaccacg tccacgtccg gttctgcgaa acgacccatc cgctctcgac gtacgactgc
     1981 tgaatctcgc gaccgtcgtc agccgcgatc gtccgacacc gcagcgccgc tcgctccgaa
     2041 cacgtagcgg cccagcttca tgccccgcgg gagcaggatc ggcaacgtct cgacgaggcg
     2101 gaacccgagg gattcgtagc gggtccggct cttcgggtcg gcatatgcgt acatcgacgt
     2161 ggcgcccccc tcgcggaaga atcgcacgat gtccgcctcg tactccgctt gcggcaccgt
     2221 gacgccgttc aggacggacc gggagccgtt gtagagctgc atcccgaatg acaggctgcc
     2281 ggcggtctcc tggtccccga cgtcgcgggt ggcggcgatc tgcgcgtcgc ggtaggtgaa
     2341 tgtcggccgg cggtagacga acgccaggtc cgcataggcg aggggtacga ggatcgccgc
     2401 ggcgagtacg agcgccgcaa gcgtcgtgaa ccgtgggcgc cggacgatca gcagggcgac
     2461 gagggccgcg gcgccgacga gcccggcgac gaggacgatc cgggcgagga tcgagccgtg
     2521 cggcgggacc ttcccgaacg ggctcgccca ggccgcgacg aacaccgcgc cggtcagcca
     2581 cacggtcgcg gcgacgagcc gccggtgatc cgcggtcacc cacgcctgga agcggcgcca
     2641 gccgaacacg ccggcagcgg cgacgggcag ggcgaagatc atgaccatga tgaatttgcg
     2701 ctcggggtag tcggccacga aggacgtctg ggcgaggaac gcggcgaggc ttgcggcgat
     2761 gagcaccatc caggcattcg gtcggcgaag gagcgtccag gcgaacaccg gaatactcgc
     2821 gaggacgacg ccgacgaacg ccgggtcgag ccggaagatg ttggcctcca ggatcgacgc
     2881 gatcttcgtc agcgagagcc cgttgccgcg tgacgaggtc gcgagtggcg agatccaggc
     2941 ccggtaccag tcgatcggcg agtggccgta gaccaggtag atcaggccgt agtacaaccc
     3001 ggcggcgacg acgcatccca cgaggaaggc gatcgtgtgg cgcccgaccg ccgcccaccc
     3061 gccgcgccgg tacgcccacc agacgagggc gaccagggcg cccgggacga ggaacgcgtt
     3121 gtagatgtag acgaagctca ccgcggccgc ggccgtggcg ccgaagacgc cgctccggac
     3181 caggccgtgt cgctcgccga ggaagacccc tcgggcgacc agggcgatca ggagcgtggc
     3241 aacggcgatt cgcgagatcg tcggctcgac gatccgggcc gacagcaggg acgagaagtc
     3301 gaccagcagc aggacgcaag ccgctgcgac caccagccag gcgagccttt tcggcacggc
     3361 gtcgaaccgc cgcgcctcgt cggcctggat ccggacgatc gagacgagcg acaggaatcc
     3421 gacgaggccg aacaggatcg acgacatccg gagcccccag aacgtgctgc cgaagagctt
     3481 gagggtcagg gcgacgacga cgttctggag gacgttggtc ggctgcccct cgagcggcgc
     3541 ccacgacgcg gcctggtgaa gccacgtccc gtagtggacc aggttgaacg ccggcagcga
     3601 gtaggtgaat tcgtcgatcg gcgagtagac ggtgagctcc cactgcggga tatcggcatc
     3661 gagcaggggc aggcgcgaca cgaggaaggc gacgatcagc ccgagaccca ggagggcggg
     3721 ggtccaacct ccgaagcggt ccgcgagcga tcggaggagg ggtcgggccg gcatcgggcc
     3781 aatgatgcac tgcgccgcgc ccgacccgct cgccgcctcg ggctgctgac cgcggaccgt
     3841 tcggcagcgg ttgtgccggg tgcgacgagg gcggatgcca taatggccgt catggcgaca
     3901 cgaaccccgg cgaacccgcc ggtcgagccc aggtcgacga tcggttccgg agcggtcagc
     3961 cgggtcgaac gggcgcccac gagctggggc ctgggcgacg gcgcggcgat tccgtgggag
     4021 tacgcgccag cgcccgagtc gcgcgacgtg gtgaccctca aggagcggta cgggctgttc
     4081 atcggcggca aggacgtcgc tgcttcggac ggcgagacgt tcgtgacggt caatccggcg
     4141 accgaggagc ccctcgccgt ggtcgcccgg gccaccgcgg ccgacatgga cagggcggtc
     4201 cgcgcggcac gcagcgcctt ccgccggtcc tggagcacgc tgtccggcag ggagcgcgcc
     4261 aa
//
LOCUS       DATAIW010000169        11681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_8305, whole genome shotgun sequence.
ACCESSION   DATAIW010000169 DATAIW010000000
VERSION     DATAIW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8305"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..658
                     /locus_tag="VHM48_05430"
     CDS             383..658
                     /locus_tag="VHM48_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754881.1"
                     /translation="MAIVSALAVAVVAIGVWVFANEGPGMAWFWSVAVVSIIALAVLY
                     PYAVACGHPNEGAVAVWLALFLGPLSLIILAPWALALRSRYDREQGG"
     gene            complement(691..1653)
                     /gene="thrB"
                     /locus_tag="VHM48_05435"
     CDS             complement(691..1653)
                     /gene="thrB"
                     /locus_tag="VHM48_05435"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2754882.1"
                     /translation="MVTNWLAELDGRRVTVEVPASSANLGAGYDCLGVALGLFNRVEV
                     EVRSWSRGEIELTVDGEGVGELAADRTNRCVLGIEAAMREVRGEIPEGVGWRIEMHNE
                     IPLARGLGSSAAATVAGLVAGNSLLSEPLTSADLLRLATAIEGHPDNAAAALLGGFTV
                     CALTDDGIESLRFDAPRDLRAVLFIPEVRLATDDMRAALPAKVPLEDAVANLSRVAIG
                     VAGMAMGRFDLLRVLTVDRLHEQYRAKAYPQLPQFVAAARKAGAIGACLSGAGSTILA
                     FSDSVRTISRIEGAFGAAAADTDLPGRIAVVSPRNLGAHLVSRA"
     gene            complement(1647..2744)
                     /gene="thrC"
                     /locus_tag="VHM48_05440"
     CDS             complement(1647..2744)
                     /gene="thrC"
                     /locus_tag="VHM48_05440"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2754883.1"
                     /translation="MSPLPGVGRIARPSLVERYRSFLPVTDATPVLSLGEGMTPLVHL
                     RRLGARLGLANLHAKIEGQNPTGSFKDRGMVVAVAKAVERGARSIICASTGNTSASAA
                     AYGAAHGLEVVVVLPAGKIAMGKLLQAIVAGARVVAVDGNFDAALRVVRALAEQDDHP
                     VTLVNSVNPFRLEGQKTGAFEVCDDLGRAPDILAIPVGNAGNISAYWAGFREYAAAGL
                     VATTPAMYGFQAAGAAPLVSGRPVEQPETIATAIRIGNPASWAKAIAARDESGGRIDA
                     VTDDEILAAYRALATEEGIFCEPSSAASVAGIMKLVSVGSAAIAPDALVVCVLTGTGL
                     KDPTTAESMVGAGQVIQAEPTVGAVAVALGW"
     gene            complement(2741..3784)
                     /locus_tag="VHM48_05445"
     CDS             complement(2741..3784)
                     /locus_tag="VHM48_05445"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX2754884.1"
                     /translation="MSRAPLRVAVLGAGTVGREVIRALVERGSELIAADGAPMELVGI
                     AVRDLARARAGGLSAALLTDAPAHLVAADETDVIVELMGGDEPAHTLIAAALGAGKSV
                     VTANKHVIAHHGPELEATARRTGAALRFEAAVGGGIPVLGPLATELAANHVRAVRGIV
                     NGTTNYILTAMADEGRDYADVLAAAQAAGYAEADPSGDVEGLDAVNKLVILARLAFGV
                     WLDPSSVATCPDDGPGAGRSGITGIGAADIAAAAAAGRTIKLIAEARRDADGSISAAV
                     LPTAVPADSPLGRTGGVLNRIEVEADPVGTVAFSGPGAGGPATSSAVLGDLVAISREL
                     GSTWAGLPPAAAR"
     gene            complement(3935..4693)
                     /locus_tag="VHM48_05450"
     CDS             complement(3935..4693)
                     /locus_tag="VHM48_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2754885.1"
                     /translation="MTALPTVRPAIAVIESTAPATDEELPRGSWGAALIRDIILGGQD
                     GLVNVLGLVLGMAVATGSARVVITAGLAAMLAESIAMAGVAFTSTGAERQAGSMLRAA
                     LAGRRDQNLAARTAARAERLASAGLPPELIATIDGEVSAEAAAWQAQLDASRAALAPI
                     RETRPLRAAAVVGLSTACGSSVPLLPFIFLPIAVAPPVALAVATVVLATAGLQRARLA
                     GGSMIRSALEMVLIGVVSAIAGALIGHLIGGPVG"
     gene            4791..5816
                     /gene="trpS"
                     /locus_tag="VHM48_05455"
     CDS             4791..5816
                     /gene="trpS"
                     /locus_tag="VHM48_05455"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2754886.1"
                     /translation="MTTDLGPGAARAPLAANPLRPRTFSGIQPSGTVHLGNELGAIRN
                     YVALQDQYEAIYCIVDYHALTSTHDPAVLRQRTREMAAGLLALGLDPERCTLFVQSHR
                     PEHAELAWLLCTVTPVSWLERTPTYKEKKVQQPDDINHGLLTYPVLQAADIVIYKASL
                     VPVGRDQAAHLELSREIVRAFNSRYGETFPEPKAVYTEAPTVLGTDGVKKMSKSVGNT
                     IDILGEPDLIRKQVMSMVTDTQRILRTDPGRPEVCNVCQLHRFFGPDWEEIQEGERTA
                     RTGCVDTKKLLAERIIRHYAGARERYAELMAHPAEVDGALEAGADRLAPVAAATLAEV
                     KEKMGLR"
     gene            6101..7297
                     /locus_tag="VHM48_05460"
     CDS             6101..7297
                     /locus_tag="VHM48_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2754887.1"
                     /translation="MTDRQRRWLTAVLVLGAIVLAMIAIEIVATVFFALGDVILVFFL
                     AWLLAFILSPVVAGLTRLVPILPRIGAVVLVYATMVGAIVLVVVLVAGALAQSITAFI
                     TNVPSLRGDLPHLLAPWQDRLNAIGLDRVDIVAQAQSFLDNLATYAAQLAGPVQQLAV
                     ASLGALGNLLIVLILSLYMVVDRDAILSFFFRIVPPSMKEEARLLESSVARSFGGFLR
                     GQAILGVVYAAVAAVTSALLGLPYLAVTTAAAGGLMAIPFFGPFVAWAPPVIVAILAI
                     PAATLPTAILMGVGWMIVMNGLQPRLMQEAVGIHPIVVLGSVLIGSKIAGITGAIFGI
                     PIAAVLSAFFFHFLILNREPSPIASRAARRLEAREGRPIRVPQEPVPGVDPDVTGVSG
                     TPETSG"
     gene            7302..8396
                     /gene="surE"
                     /locus_tag="VHM48_05465"
     CDS             7302..8396
                     /gene="surE"
                     /locus_tag="VHM48_05465"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX2754888.1"
                     /translation="MSDPGRPRNPDRAPDRADEGPGDKPDMTRERARTERRDLDKERG
                     EPVAGGAARGGPRALHPNARPLHDWSTRPKILITNDDGVESRGLLALKQALEPIGDTT
                     VVAPDTNQSAVGHQKTLMRPLRIRERTLADGSLAYSVDGSPTDAVSIAFLGYFGHGFD
                     LVAAGINYGANLGDDITYSGTVSAAMEAVINGCPAFAISQEYYEHPDFALATVAASIV
                     ARNILEHGLSRGELVNVNVPGVTIAECQGIEVTRLGRRVYQDQLVERLDPRGIPYFWI
                     GGPPPSGLAIEGTDFHAVINRRIAITPIHLDLTGRRLLRRLSTWAWPSPADVAAQAVA
                     IPASPPPGRDEPVAVAAEERLGDEEQIERR"
     gene            complement(8485..10143)
                     /locus_tag="VHM48_05470"
     CDS             complement(8485..10143)
                     /locus_tag="VHM48_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2754889.1"
                     /translation="MARIESSTAATGGPVVATSRGYDAIVVGGGHNGLVTAAYLARAG
                     KRTLLLERRGHVGGAAETGELGGVRVPRLADTVGRIRPSVVKDLDLRSHGLQLVAPDV
                     RVFAPQPDGRAVTLWADQARTVDGLRASSDHDADAYPDFDRLVRSLGRFLADLAAETP
                     PDIKAPGIGDALMGLKLGRTFRGLGKHDSHTILRVLPMAVADFVAEAFESDALRAAIA
                     WRGVRYSFLGPWSAGSTAILLADGAGNDGGAAGQTVFAIGGPGALSEALAAAARAAGA
                     EIRCDADVAAITSRDNHVTGVALASGEEIPAPIVVSGLDPKRTLVGLADPVTIGPSMR
                     WRAGNYRTPGVVAKVNLVVDRLPRFTAAAGDDEQLLRGRILAAPGIDAIERAFDAAKY
                     GRTSDTPVLEATIPSLVDPTLVDGAPDGTHVVSISAQYAPFKLRGGTWDDDGRADALG
                     DKVVAVLDGLAPGFGASVRAREVITPLELERDYGLSGGHPLHGEQALDQFFLWRPFLG
                     SGRYRLPIDGLYLCASGAHPGGGITGQPGQNAAREILSDLKRRR"
     gene            complement(10143..>11681)
                     /locus_tag="VHM48_05475"
     CDS             complement(10143..>11681)
                     /locus_tag="VHM48_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2754890.1"
                     /translation="AYLARADWKVLVLERRHVLGGAAVTEEVYPGFRFSVCSYVVSLL
                     RPEIIRELDLPRHGLEILPLDGTFTPLDDDYLWRVNDHGRTIRELRRWSRSDAEAYEE
                     YGQLMVEMARFIKPILGITPPDLTSLDPRPLLPLGGLIRSFQQLPERQQAVFVQLMTM
                     SAADFLDQWFETDPLKATMSASGIIGTYLGVRSPGTAYVLLHHYMGEIDGAFRAWGIP
                     KGGTGGISNAIGSAARALGAEIRMEAPVERILVKDGRAVGVVLRDSGEEIRADVVLSS
                     VDARLTFLGLLEPGTLDGEFEEKIRRFKFRGSSGKVNMAVDRLPEFPSLPGVGEHLRG
                     AISFSPSVDEMEQAYDDAKYGHFSKRPYVDMIIPTLVDPTMAPPGKHVVSCFVQYAPY
                     KLAPELGTWDDQREAFGDAVVDRISEFSPGFRDSILFRNVQTPLDIERTTGLTEGNIF
                     QGELTLEQLFFNRPVPGYARFRTPVRDLWLCGSSTHPGGGIMGANGRIAALEVLRSLG
                     RRAA"
BASE COUNT         1679 a         4241 c         4136 g         1625 t
ORIGIN      
        1 tcggccagac gctgcacggg atggtcgaca cgctcaaccg ggcgctgccg ggcgggatgc
       61 tcgcggccgt cgtcttcgtc atcctcgtcg tcgccgcctg gcagctcgcc aagcgctgag
      121 ccctcgcctc ggccggcccg ccgcgccgcg cccgatcgcc tgcaggaagc cgtccatgca
      181 cccgagtctg cgctgtgcgg tcaatcgccc cggaccgcgc aagacctccc ccgaccgcgg
      241 tccgcgccgg tcggagacgc gacccagtcg ggccagatgg cacgatgttg cctcgagcgg
      301 aacgacgacg atcgtgggcg aacgggttca ggaggtgacg atggacgctg gccgatccga
      361 tcgtcggcgt gcgactcgcc agatggcgat cgtttccgcg ctggcggttg ctgtggtcgc
      421 gatcggcgtc tgggtattcg cgaacgaagg ccccggaatg gcctggttct ggtccgtggc
      481 ggtcgtgtcg atcatcgcgc tcgcggtcct gtatccctac gccgtcgcgt gcggacaccc
      541 gaatgagggg gccgtggcgg tctggctcgc cctcttcctc ggaccactct cgctgatcat
      601 cctggcgccc tgggcgctcg ctctccggtc acgatacgac cgggagcaag gcgggtaggc
      661 cccggcacgg atccggaact cgatgcccga tcaggcgcgc gacaccaggt gggcgccgag
      721 gttgcggggc gagacgaccg cgatccggcc gggcaggtcc gtgtccgcgg cggcggcgcc
      781 gaacgccccc tcgatccggc tgatcgtccg gaccgagtcc gagaaggcga ggatcgtcga
      841 gccggcgccg gagagacacg ccccgatcgc ccccgctttg cgggcggcag cgacgaactg
      901 cgggagttgc ggataggcct tcgcccggta ttgctcatgg aggcggtcga ccgtcaggac
      961 ccggagcagg tcgaagcgac ccatcgccat cccggccacg ccgatcgcga cccggctcag
     1021 gttggcgacc gcgtcctcga gcggcacctt ggccggcagg gcggcccgca tgtcatcggt
     1081 cgcgagccgg acctcgggaa tgaacaggac ggcccggagg tcgcgcggcg cgtcgaaccg
     1141 gagcgactcg ataccgtcgt ccgtgagggc gcagacggtg aagccgccga ggagcgccgc
     1201 ggccgcgttg tcgggatggc cctcgatcgc ggtcgcgagc cggagcaggt ccgccgacgt
     1261 caacggctcg ctgagcaggc tgttgccggc gacgagcccg gcgaccgtcg ccgcagccga
     1321 cgacccgagg ccgcgggcca gggggatctc gttgtgcatc tcgatccgcc acccgacgcc
     1381 ctcggggatc tcgccgcgga cctcccgcat cgccgcctcg attccgagga cgcagcggtt
     1441 cgtccgatcc gccgcgagct cgccaacgcc ctcgccgtcg acggtcagct cgatctcgcc
     1501 gcggctccag ctgcgaacct cgacctcgac ccggttgaac aaccccaggg ccacgccgag
     1561 acagtcgtac cccgccccga ggttggcgct cgacgccgga acctcgaccg tcacccgccg
     1621 gccgtcgagc tcggcgagcc agttcgtcac catccgagcg cgaccgccac cgcgccgacc
     1681 gtcggctcgg cctggatgac ctgccccgcg ccgaccatcg actcggcggt cgtcgggtcc
     1741 ttgagcccgg tcccggtcag gacgcagacg acgagcgcgt ccggggcgat ggcagcggaa
     1801 cccaccgaca cgagcttcat gatccccgcc acgctggccg ccgacgacgg ctcgcagaag
     1861 atgccctcct cggtggcgag agcgcggtag gcggcgagga tctcgtcgtc ggtgacggcg
     1921 tcgatccggc cgccgctctc gtcgcgggcg gcgatcgcct tggcccacga cgccgggttg
     1981 ccgatccgga tcgcggtcgc gatggtctcc ggctgctcga ccggccggcc gctcacgagc
     2041 ggcgccgcgc cggccgcctg gaagccgtac atcgccggtg tcgtcgcgac cagcccggcc
     2101 gcggcgtact cgcggaaccc ggcccagtag gcgctgatgt tgccggcatt gccgaccggg
     2161 atcgcgagga tgtcgggcgc ccggccgaga tcgtcgcaga cctcgaacgc gccggtcttc
     2221 tggccctcga gccggaacgg gttgaccgag ttgacgagcg tcaccgggtg gtcgtcctgc
     2281 tcggcgaggg cgcggacgac ccgcagcgcg gcgtcgaagt tgccgtcgac cgcgacgacg
     2341 cgagccccgg ccacgatcgc ctggaggagc ttgcccatcg cgatcttgcc ggccggcagg
     2401 acgacgacga cctcgaggcc gtgggccgcg ccgtaggccg ccgccgatgc cgacgtgttc
     2461 ccggtcgagg cgcagatgat ggagcgcgcg ccgcgctcca cggccttggc caccgcgacg
     2521 accatgcccc ggtccttgaa cgagccggtc gggttctggc cctcgatctt ggcgtggagg
     2581 tttgccagcc cgagacgcgc cccgaggcgg cggaggtgga cgagcggggt catgccctcg
     2641 ccgaggctca ggaccggcgt cgcatcggtg acgggcagga acgagcggta gcgctcgacg
     2701 aggctcggcc gggcgatccg tccgacgccg gggagcgggc tcatcgcgcg gctgccggcg
     2761 gcaggccggc ccacgtgctg ccgagctcgc gggagatcgc gacgagatcg ccgagaaccg
     2821 cgctcgaggt cgccggtccg ccggcgcccg ggccgctgaa tgccaccgtc ccgaccggat
     2881 cggcctcgac ctcgatccgg ttcaggaccc cgccggtccg gccgagcggc gaatcggcgg
     2941 gaaccgcggt cggcaggacg gctgccgaga tcgagccgtc ggcgtcgcgg cgcgcctcgg
     3001 cgatcagctt gatcgtccgg cccgcggccg ctgccgcggc gatgtcggcg gcaccgatcc
     3061 cggtgatccc ggaccggccg gcgcccggac cgtcgtccgg gcaggtcgcg accgaggacg
     3121 ggtcgagcca cacgccgaac gcgagcctgg cgaggatgac gagcttgttg accgcgtcga
     3181 ggccctcgac gtcaccgctc gggtcggcct ccgcgtagcc cgcggcctgg gctgcggcga
     3241 ggacgtcggc atagtcgcgg ccctcgtcgg ccatcgccgt gaggatgtag ttggtcgtgc
     3301 cgttgacgat gccgcggacg gccctgacgt ggttcgccgc gagctccgtc gcgagtggcc
     3361 cgaggacggg gatcccgccg ccgaccgccg cctcgaaccg gagggccgcg cccgtccggc
     3421 gggccgtcgc ttccagctcg gggccgtggt gggcgatcac gtgcttgttc gcggtcacga
     3481 ccgacttccc ggctccgagc gccgccgcga tcagggtgtg ggcaggctcg tcaccgccca
     3541 tgagttcgac gatcacgtcg gtctcatcgg ccgccacgag gtgggccgga gcatccgtca
     3601 ggagcgcggc cgacagtcct cccgctcggg cccgggcgag atcgcggacg gcgatgccga
     3661 cgagctccat gggagctcca tcggcggcga tcagctccga tccacgctcg acgagggcac
     3721 ggatgacctc gcggccgacc gtgcctgcgc cgaggacggc gacacgcagc ggcgcgcggc
     3781 tcatcggcaa tcggctcgtg acggcagagt catcgggccg aatggtagcc gagcccgaag
     3841 gccatttcgg aacaacgggc gccgcgcccc gggtcggcgc gtcgtcaaca ccgtcgaacc
     3901 tgctcctccc gctggtcgca acgggccgat cggactagcc gacgggtccg ccgatgaggt
     3961 gtccgatcag cgcgccggcg atggccgaca cgaccccgat caggaccatc tccagcgcgg
     4021 atcggatcat cgatccgccg gccaggcggg cccgctggag gcccgcggtg gcgaggacaa
     4081 cggtcgccac ggcgagggcg accggcggcg ccacggcaat cggcaggaag atgaacggga
     4141 gcagcggcac cgatgagcca caggcggtcg agagcccgac aacggcggct gcccggagcg
     4201 gccgggtctc gcggatcggt gccaatgcgg cgcgagaggc gtcgagctgg gcctgccagg
     4261 cggctgcttc ggcggagacc tcgccatcga tcgttgcgat cagctcgggc ggcagcccgg
     4321 ccgaagcgag ccgctccgcg cgggcggccg ttcgagccgc gagattctgg tcgcggcgac
     4381 cggccagggc cgcccggagc atggagccgg cctgtcgctc ggccccggtc gaggtgaatg
     4441 cgacgccggc catcgcgatg gactcggcaa gcatcgccgc gagcccggcg gtgatcacga
     4501 cccgggcgga gcccgtggcg acagccatcc cgaggacgag gccgagcacg ttcaccagcc
     4561 catcctggcc accgaggatg atgtcgcgga tcagggccgc accccagctg ccgcgcggca
     4621 gctcttcgtc cgtggcgggc gccgtcgact cgatgacggc aattgcgggg cgaacggtcg
     4681 gaagggcggt catgaccgaa gcctcgcccc tgggcccgtg tcgccacacc ggccgagcgg
     4741 cccgcggagt cgggccatcc ggcccgatgg gtccggtaca ctcgccgccc atgacgaccg
     4801 acctcggacc gggtgctgct cgcgccccgc tcgccgccaa cccgctgcgg ccgcggacct
     4861 tctccggcat ccaaccgtcc gggaccgtcc atctcgggaa cgagctcggc gcgatccgca
     4921 actatgtagc gctccaggac cagtacgagg cgatctattg catcgtcgac taccacgccc
     4981 tgacgagcac ccacgatccg gccgtcctcc gccagcgcac gcgcgagatg gccgccggac
     5041 tgctcgctct cggtctcgac cccgagcgct gcacgctctt cgtccagagc caccgccctg
     5101 agcacgccga gctcgcctgg ctgctctgca ccgtcacccc ggtcagctgg ctcgagcgga
     5161 cgccgacgta caaggaaaag aaggtccagc agccggacga catcaaccac ggcctgctca
     5221 cctacccggt cctgcaggcg gccgacatcg ttatctacaa ggcgtcgctc gtgccggtcg
     5281 ggcgggacca ggccgcccac ctcgagctca gtcgcgagat cgtccgtgcg ttcaactcgc
     5341 gctacggtga gacgtttccg gagccgaagg ccgtctacac cgaagccccg accgtcctgg
     5401 gcaccgacgg cgtgaagaag atgagcaagt cggtcggcaa cacgatcgac atcctcggcg
     5461 agccggacct gatccggaag caggtgatgt cgatggtcac cgatacccag cggatcctgc
     5521 ggaccgaccc gggccggccg gaggtctgca acgtctgcca gctccaccgc ttcttcggac
     5581 ccgactggga ggagatccag gagggcgaac gaacggcccg gaccggctgc gtcgacacga
     5641 agaagctcct cgccgagcgg atcatccgcc actacgcggg cgctcgcgaa cgctacgccg
     5701 agctgatggc ccacccggcc gaggtcgacg gcgccctcga ggccggcgcc gaccgcctcg
     5761 ccccggtcgc cgccgcgacc ctggccgagg tcaaggagaa gatgggcctg cggtaggtcg
     5821 cgacatcgga caccgcgcgc gagatcggtg gttccgtccg tttcgaatag ccgaatgtcg
     5881 agcgcgcctg cgcgatcggt cagcgcgcaa gctggtccgg gccccagcgg ctcacctcgg
     5941 atattcggct attcgcaggc agctggggcg tgtgatcggc gggagtgtcc ggcgcgggtc
     6001 gcgtctcgcc tcgacgcggg tgaccgggtg agcagcgggg aaccggcggc gtctaccatg
     6061 gcagcgggtg atggatgacg atcgtccgag gatggtgcgc gtgaccgacc gacaacggcg
     6121 ctggctgacc gcggtcctgg tgctcggcgc gatcgtcctc gcgatgatcg cgatcgagat
     6181 cgtcgcgacg gtgttcttcg ccctcggcga cgtcatcctc gtcttcttcc tggcctggct
     6241 cctcgccttc atcctgagcc cggtcgtcgc gggtctgacc cgcctcgtgc cgatcctgcc
     6301 gcggatcggg gccgtcgtcc tcgtctacgc cacgatggtc ggcgcgatcg tgctcgtcgt
     6361 cgtcctcgtc gcaggtgccc tcgcccagtc gatcacggcc ttcatcacga acgtcccgag
     6421 cctccggggc gacctgcccc atctcctcgc cccgtggcag gaccggctca acgcgatcgg
     6481 gctcgaccgc gtcgatatcg tcgcccaggc acagagcttc ctcgacaacc tcgcgacata
     6541 cgccgcccag ctggcggggc cggtccagca gctggcggtc gcgagcctcg gagcgctcgg
     6601 caacctgctc atcgtcctga tcctgtcgct gtacatggtc gtcgaccgtg acgcgatcct
     6661 gtcgttcttc ttccggatcg tgccgcccag catgaaggag gaggcgcgcc tcctcgagag
     6721 cagcgtcgcc cgctcgttcg ggggcttcct ccgcggccag gcgatcctcg gcgtcgtcta
     6781 cgccgcggtc gcggccgtga ccagcgcgct gctcgggctg ccgtacctgg ccgtcacgac
     6841 ggctgccgcg ggcggattga tggcgatccc gttcttcggc ccgttcgtcg cctgggcgcc
     6901 accggtgatc gttgccatcc tcgccatccc cgcggcgacg ctgccgaccg ccatcctgat
     6961 gggcgtcggc tggatgatcg tcatgaatgg cctccagccg cgtctgatgc aggaagcggt
     7021 cgggatccat ccgatcgtcg tcctcggctc ggtcctgatc gggagcaaga tcgccggcat
     7081 caccggggcg atcttcggga ttccgatcgc ggcggtcctg tcggcattct tcttccactt
     7141 cctgatcctg aaccgcgaac cgtcgccgat cgcgtcccgt gcggcgcgaa ggctggaggc
     7201 gcgcgagggc cggccgatcc gggtacccca ggaaccggtt cccggtgtcg acccggacgt
     7261 gaccggcgtc agcggcacgc ccgaaacgag cggctgatcc gatgagcgac cccgggcgcc
     7321 cgcggaaccc cgaccgcgcg cccgaccgcg ccgacgaggg cccgggcgac aagccggaca
     7381 tgacccgcga gcgtgcccgg accgagcggc gcgacctcga caaggagcgc ggcgagccgg
     7441 tcgccggcgg cgccgcccgg ggcggaccgc gggccctcca tccgaacgct cggccgctcc
     7501 acgactggtc gacccggccg aagatcctga tcaccaacga cgacggcgtc gagtcgcgcg
     7561 ggctgctcgc cctcaagcag gcgctcgagc cgatcggcga cacgaccgtc gtcgcgccgg
     7621 acacgaacca gagcgccgtc ggtcaccaga agaccctgat gcgaccgctc cgcatccgcg
     7681 agcggaccct cgccgacggc tcgctcgcgt actccgtcga cggcagcccg accgacgcgg
     7741 tcagcatcgc cttcctcggc tatttcgggc acggcttcga tctcgtcgcc gcgggcatca
     7801 actacggcgc gaacctcggc gacgacatca cgtactccgg aaccgtgtcg gccgcgatgg
     7861 aggccgtgat caacggttgc ccggcgttcg cgatcagcca ggagtattac gagcaccccg
     7921 acttcgccct ggccaccgtc gccgcctcga tcgtcgctcg caacatcctc gagcacggcc
     7981 tctcacgcgg cgagctcgtc aacgtcaacg tgcccggcgt cacgatcgcc gagtgccagg
     8041 ggatcgaggt gacccggctc gggcgacggg tctaccagga ccagctcgtc gagcgcctcg
     8101 acccacgtgg cattccctac ttctggatcg gcggaccgcc gccgtccggc ctcgccatcg
     8161 aggggaccga cttccatgcg gtcatcaacc gccggatcgc gatcacgccc attcatctcg
     8221 acctgacggg gcggcggctg ctccgtcggc tgagcacctg ggcgtggccg tcgccggccg
     8281 atgtcgcagc ccaggcggtc gccattccgg cttcgccgcc gccgggtcgc gacgagccgg
     8341 tcgccgtcgc ggccgaggag cggctgggcg acgaagaaca gatcgagcgc cgctgaaacg
     8401 acccaatttc atcggcctcc gcgggcctgc ggaccgggcg ttgtgacgat gccgactctg
     8461 cgcgctcgtc ctcggccgac gcgatcagcg ccgccgtttc aggtcggaga ggatctcgcg
     8521 ggcggcgttc tggcccggct ggccggtgat tccgccgccc ggatgggcgc ccgaagcgca
     8581 caggtagagt ccgtcgatcg ggagccggta gcgcccgctg ccgaggaacg gacgccacag
     8641 gaagaactgg tcgagggcct gctcgccgtg gagcgggtgg ccgccgctga ggccatagtc
     8701 gcgctcgagc tcgagcggcg tgatgacctc gcgggcacgg acgctcgcgc cgaacccggg
     8761 cgcaagcccg tcgaggacgg ccacgacctt gtcgccgaga gcgtccgccc ggccgtcgtc
     8821 gtcccaggtc ccgccgcgca gcttgaaggg cgcgtactgg gcgctgatgc tgacgacgtg
     8881 ggtcccgtcg ggtgccccgt ccacgagcgt cggatcgacg agcgacggga tcgtcgcctc
     8941 gaggaccggc gtgtcggagg tccgcccgta cttggcggcg tcgaaggctc gctcgatcgc
     9001 gtcgatcccc ggcgcggcca ggatccggcc gcggaggagc tgctcgtcgt cgccagcggc
     9061 ggccgtgaac cggggcagcc gatcgacgac gaggttgacc ttggccacga cgcccggcgt
     9121 ccggtagttg ccggcccgcc agcgcatcga cggtccgatc gtgaccgggt cggcgagacc
     9181 gacgagcgtc cgcttcggat cgaggccgga cacgacgatc ggcgccggga tctcctcgcc
     9241 cgacgcgagg gcgacgccgg tcacgtggtt gtcgcgcgag gtgatcgcgg cgacgtccgc
     9301 gtcgcagcgg atctcggcgc cggccgcccg ggcagcggcc gcgagcgcct cggacagcgc
     9361 gcccggcccg ccgatcgcga agaccgtctg gcccgccgct ccgccgtcgt tgccggcacc
     9421 gtcggcgagc aggatcgcgg tcgagcccgc cgaccacggc ccgaggaacg aataccggac
     9481 accccgccac gcgatcgcgg cccgcagcgc gtcgctctcg aacgcctctg cgacgaagtc
     9541 ggcgaccgcc atcggcagga cccgcaggat cgtgtgcgag tcgtgcttgc cgaggccgcg
     9601 gaacgtccgc ccgagcttga gccccatgag agcgtcgccg atgccgggcg ccttgatgtc
     9661 cggcggcgtc tcggccgcga ggtccgccag gaaccttccg agcgagcgga cgagacggtc
     9721 gaagtccggg tacgcgtcgg cgtcgtggtc cgacgaggcg cgcaggccgt cgaccgtccg
     9781 cgcctggtcg gcccagagcg tgactgcccg accgtcgggc tgcggggcga agacgcggac
     9841 atccggcgcg acgagctgca atccgtggga gcggaggtcg agatccttga cgaccgacgg
     9901 ccggatccgc ccgaccgtgt ccgcgaggcg cggcacccgc accccgccga gctcgcccgt
     9961 ctcggccgcg cccccgacgt ggccgcgccg ctcgaggagg agcgttcgct tgccggcccg
    10021 ggcgaggtag gccgcggtca cgaggccgtt gtgcccgccg ccgacgacga tcgcgtcgta
    10081 gccccggctc gtcgcgacga ccgggccgcc ggtcgcggcg gtcgagctct cgatcctcgc
    10141 catcacgccg ccctccggcc gagcgagcgg agcacctcga gggcggcgat ccgaccgttc
    10201 gcgcccatga tcccgccgcc cggatgggtc gacgagccgc acagccagag gtcccggacg
    10261 ggagtcctga accgggcgta gccgggaacc gggcggttga agaagagctg ctcgagggtc
    10321 agctcgccct ggaagatgtt gccctcggtc agtccagtcg tgcgctcgat gtcgagcggc
    10381 gtctggacat tccggaacag aatgctgtcc cggaagccgg gcgagaactc gctgatccgg
    10441 tcgacgaccg cgtcgccgaa ggcctcgcgc tggtcgtccc acgtcccgag ctccggcgcg
    10501 agcttgtacg gcgcgtactg gacgaagcag ctgaccacgt gcttgccggg cggggccatc
    10561 gtcgggtcga cgagggtcgg gatgatcatg tcgacgtacg gcctcttcga gaagtggccg
    10621 tacttcgcgt cgtcgtaggc ctgctccatc tcgtcgaccg acgggctgaa cgagatcgcg
    10681 ccgcggaggt gctcccccac cccgggcagg ctcgggaact ccggcagccg atcgacggcc
    10741 atgttgacct tgcccgacga cccacggaac ttgaaccggc ggatcttctc ctcgaactcg
    10801 ccgtcgaggg ttcccggctc gagcagcccg aggaacgtca gacgggcatc gaccgacgag
    10861 aggacgacgt cggcccggat ctcctcgccg ctgtcccgca ggacgacgcc gacggcccgg
    10921 ccgtccttga cgaggatccg ctcgaccgga gcctccatcc ggatctcggc gccgagagct
    10981 cgagccgccg acccgatcgc gttcgagatc ccgccggtcc cgcccttcgg gatgccccat
    11041 gcccggaagg cgccgtcgat ctcacccatg tagtggtgga ggaggacgta ggcggtgccc
    11101 ggcgagcgca ccccgaggta cgtcccgatg atccccgacg cgctcatcgt cgccttgagc
    11161 ggatccgtct cgaaccactg gtcgaggaag tccgccgcgg acatcgtcat cagctggacg
    11221 aagacggcct gctgtcgctc cgggagctgc tggaaggagc ggatcagccc gccgagcggc
    11281 agcagcggtc gtggatcgag cgaggtcagg tcgggcgggg tgattccgag gatgggtttg
    11341 atgaaccggg ccatctcgac catcagctgg ccgtattcct cgtaggcctc agcgtccgaa
    11401 cgggaccagc ggcggagctc gcggatcgtg cggccgtggt cgttgacgcg ccacaggtag
    11461 tcgtcgtcga gtggcgtgaa cgtgccgtcc agcggcagga tctccaggcc gtggcgcggc
    11521 aggtccagct cgcggatgat ctccggccgc agcaggctca cgacgtagga gcagacgctg
    11581 aaacggaagc ccgggtagac ctcctcggtc acggctgcgc cgcccaggac atgacggcgc
    11641 tccagcacca gcaccttcca gtcggctcgc gcgaggtatg c
//
LOCUS       DATAIW010000170         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_98049, whole genome shotgun sequence.
ACCESSION   DATAIW010000170 DATAIW010000000
VERSION     DATAIW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98049"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1035)
                     /locus_tag="VHM48_05480"
     CDS             complement(466..1035)
                     /locus_tag="VHM48_05480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2754891.1"
                     /translation="MNEDHVVGLPLGLRIRPETPADAEEIGRLHDLAFDDPQVARLVE
                     AIRSSSWYRPELALVADLPGQPGSPIVGHVLISGTELVTEQGDHVTILMLSPLGVLPA
                     HQGRGIGSALNRAVLALADAGSEPLVIVQGHPDYYPRFGFVRGRTIGVLPPEHLGAID
                     KAWMVRRRPGAPELRGRVTYPPAFLELDG"
     gene            complement(1032..>2192)
                     /locus_tag="VHM48_05485"
     CDS             complement(1032..>2192)
                     /locus_tag="VHM48_05485"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX2754892.1"
                     /translation="VHGFTVPAINIRAQTFDMARTIYETAEAADVGAVIIELARSEQT
                     YTFQRPVDFATAVLAGAVAAGWRGPVFLQGDHYQFNMKKYTADPEAMTEEIRRACRLA
                     IDAGYRNIDIDSSTLVDLALDGRDAQQHENYVRAAELTALIRSLETDGVTVSVGGEIG
                     EVGKENSNEEELLAYLDGYRRELDGRAPGATGISKVSVQTGTSHGGVPLRDGSVAEVK
                     LDFEVLRRLGEVARSYGLAGAVQHGASTLPDELFHRFPAVETAEIHLATGFQNALYEH
                     PAFPAELHREIESWCYANAIDERKPDQSDQQFVYTSRKKAIGPFKRALWDLATKDEIL
                     ASQRRKIGFLFTELGVNRSREMVDRYIHPVEQRTPPPDGLREAAATGAGAGG"
BASE COUNT          307 a          756 c          802 g          327 t
ORIGIN      
        1 cacgggtcac tgcctcccac gtgacgaggc ggcgcgtcgg aaccccggcg cgccgcccgt
       61 ccattgcctc catgacgatc ggcgtcagcc gatcacggcc tcgcccgtgg cgagatcggc
      121 ttccgtgtcc gcggctcgac ctcgccgcgg ttgcggccga ggatggcacc ggcccacggt
      181 gccgtcaacg ctgttgcgcc cgacacgctg ttgcgcccgt caacgctgtt gcgcccgacg
      241 ccgagcggga atgggatggc gtgcggagtg cgcaccctgt gagcactgtg acgaaggacg
      301 gagcggtggt tcgtcacctg cccagccgct gggcgaaacg gcgactgacc atccggatcg
      361 aggcgtctgg tcccgccagc cggacgcgcc ttggtcgcaa cgcgcagccg gtgcgcaggc
      421 gacggcacgc agagcggccg actccacccg ccggtcagag cgaggtcagc cgtccagctc
      481 gaggaaggcc ggcggatacg tgacgcggcc gcgaagctcc ggggcgcctg gccggcggcg
      541 gaccatccag gccttgtcga tcgccccgag gtgctccggc ggcaggacgc cgatcgtccg
      601 gccgcggacg aatccgaacc gcgggtagta gtcggggtgg ccctggacga tgacgagcgg
      661 ctcagagccg gcgtcggcga gggcgaggac ggcccggttg agcgccgacc cgatcccccg
      721 gccctgatga gcgggcagca ccccgagcgg cgacagcatc aggatcgtga cgtggtcgcc
      781 ctgctcggtc accagctcgg taccgctgat cagcacgtgc ccgacgatgg gcgagcccgg
      841 ctgacctggc aggtcggcga cgagggcgag ctctggccgg taccacgacg acgaccggat
      901 cgcctcgacg agccgggcga cctgcggatc gtcgaacgcc aggtcgtgga ggcgcccgat
      961 ctcctcggcg tcggccggcg tctccggccg gatccggagg ccgagcggca ggccgacgac
     1021 gtggtcctcg ttcagccgcc ggctccggcc ccggtcgccg cggcttcgcg gaggccgtca
     1081 gggggcggag tccgctgctc gaccgggtgg atgtaccgat cgaccatctc ccgcgagcgg
     1141 ttcacgccga gctcggtgaa caggaagccg atcttgcgcc gctgggaggc caggatctcg
     1201 tccttggtcg cgaggtccca cagcgcccgc ttgaacgggc cgatcgcctt cttgcgcgac
     1261 gtgtagacga actgctggtc ggactggtcg ggcttgcgct cgtcgatggc gttcgcgtag
     1321 caccacgatt cgatctcgcg gtggagctcg gcagggaacg ccggatgttc gtacagcgca
     1381 ttctggaacc cggtcgcgag gtggatctcg gcggtctcga cggccgggaa tcggtggaag
     1441 agctcgtcgg gcagggtcga cgccccgtgc tggacggcac cggccagacc gtaggatcgg
     1501 gccacctcgc cgagccgccg caggacctcg aaatcgagct tgacctcggc aacggagccg
     1561 tcccgcagcg gcacgcctcc gtgcgacgtg ccggtctgga cgctgacctt gctgatcccg
     1621 gtcgcgccgg gggcacggcc gtcgagctcg cgccgatagc cgtcgagata ggcgaggagc
     1681 tcctcctcgt tcgagttctc cttgccgacc tcgccgatct cgccgccgac gctgacggtc
     1741 acgccgtcgg tctcgagcga gcggatcagg gccgtcagct cggccgcccg gacatagttc
     1801 tcgtgctgct gggcgtctcg gccgtccaac gcgaggtcga cgagggtcga gctgtcgatg
     1861 tcgatgttgc ggtagccggc gtcgatcgcg aggcgacagg cccgccggat ctcctcggtc
     1921 atcgcctcgg gatccgcggt gtacttcttc atgttgaact ggtagtggtc gccctggagg
     1981 aacaccggcc cgcgccagcc ggccgcgacc gccccggcca gcacggcggt cgcgaagtcg
     2041 accgggcgct ggaaggtgta ggtctgctcg ctccgggcga gctcgatgat cacggcgccg
     2101 acgtcggccg cctcggcggt ctcgtagatc gtccgggcca tgtcgaaggt ctgggcccgg
     2161 atgttgatcg cgggcacggt gaacccgtgg ac
//
LOCUS       DATAIW010000171         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212273, whole genome shotgun sequence.
ACCESSION   DATAIW010000171 DATAIW010000000
VERSION     DATAIW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212273"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VHM48_05490"
     CDS             <1..1449
                     /locus_tag="VHM48_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2754893.1"
                     /translation="PYDFPAVERTRLANGLSLAVIHLPGRPLVAGTLVLRNGAADEPD
                     GEGGATVLAARALTEGTERYDAIALVEAGERLGAALHADAGWDAMSIGVDVPAARLEP
                     ALELLAEVALHPTFPDAEVDRLRDERLNDLLQAEADPRRRADEVFAATIYSGGSPYRR
                     PSGGLRATVETLDAARLRAAFQRGLDPARATLIVGGDLGGIDVRAIAERLLGGWGAAY
                     GAGPTGSIVAESAVRERFVRVIHRPGSVQTEIRIGHVGLPRRISDFHALSVMGAILGG
                     LFNSRLNTKLREEKGYTYGAGAGFDLRRAAGPFAARAAVNTDVTVPAIVDMLAELDRI
                     KTAPVDASELKAARDFLVGVFPLRFETPGPVVGALAGLAVHDLSDDELTRYRPAIESV
                     TIEAVLAAAGHIRTDQAAIVLVGDADAFGADLEAAGFGPVQIERDHGPQDEGRATGVQ
                     GDLEPLDEGPAGPTEGGEEPDTGSVAAEPAVD"
     gene            1567..2085
                     /locus_tag="VHM48_05495"
     CDS             1567..2085
                     /locus_tag="VHM48_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754894.1"
                     /translation="MDVKNRLVRGAATSAVALFLVSSAVLGANAIASAPRVVDATLTT
                     SDQSAEPAESAEPTEAAESAEPTEAAETAEPTDTAEPNEPTDTAEPNEDTHSGQGGQV
                     SQDGNDQGEHGTADPTSTAGHDGDNEDASDNEDANETAEPAETPEPTDDNGGDSSGSG
                     SDSSGHDGSGHD"
     gene            complement(2199..2366)
                     /locus_tag="VHM48_05500"
     CDS             complement(2199..2366)
                     /locus_tag="VHM48_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754895.1"
                     /translation="MDVAEDAMTGADDRGRFTIDEGSERVAVAGQDGVDSGAFIDDLG
                     IVGWVEWWWIG"
     gene            2331..2789
                     /locus_tag="VHM48_05505"
     CDS             2331..2789
                     /locus_tag="VHM48_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2754896.1"
                     /translation="MRACHRVLGDIHEAEDAAQEAFVIAFRSLAGWRADGPFGAWLTR
                     IAVRIALRRAQKRRDVSWIDPTHPDRPLDLPGGIDPATSSLRAERAADVRAAVARLDE
                     PYRETVALRFFGDLSLDEIATQTGRPLGTVKTHLHRGLIRLRGVVETGAL"
     gene            2786..3481
                     /locus_tag="VHM48_05510"
     CDS             2786..3481
                     /locus_tag="VHM48_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754897.1"
                     /translation="MNGELPLHPSDEERLAAAMARELESITTMTPVQPGEGFADRVML
                     AIADEPLPQPVRAFGLALLGGHLRAAASAVGDAWRTIASSPAPLFVRAQALALVLVVL
                     VASLALAGGATVGAMGLLGNGPVTPVPSGPPPTVPVASPTPSPLPQAEDTPSASPEES
                     SDAEATETPDATETPDSQETERATTRPRTAEPTEDHGGDRSGSESGSGSGSDGGDHTP
                     SPTETDNHSGGDG"
     gene            3538..>4698
                     /locus_tag="VHM48_05515"
     CDS             3538..>4698
                     /locus_tag="VHM48_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX2754898.1"
                     /translation="MPEFKSRTYANPRRLPRRNHYQEGPDFLFVATQGGRPPESTVGD
                     GPAASVRRRRAALRRWGDGEPIGAICAEVHCSRASLFRWRTRYEAGGLEALVDRPRVG
                     RASALPPAIERLILTVRLLTYWNSRRIAAEFRRRGVWPLGHGQIDRLLDRNGSHRPSY
                     VRTPGPRYERAAVNELWHIDLKGPFYLLGATGRARTCHFAALVDDHSRFLLGIRAVPT
                     KEAVWILALLEEAIELCGVPQELMTDNGTPFVAIVRTMLSRFQRSLAELAIRHIRTQI
                     DTPWTNGKIERFWATLQAEVLDRQQLVDLAEAEAAVTAYAGYYNYHRLHGELDWQTPA
                     ERFDGTPFTDRGFGSVPALAGVADLLDASIRPDLGQAHAFCGHCGPRSPASAS"
BASE COUNT          699 a         1746 c         1626 g          627 t
ORIGIN      
        1 ccgtacgact tcccggcggt cgagcggacg cgcctcgcca atggactctc gctggccgtc
       61 atccacctcc ccggtcggcc gctcgtcgca ggaacgctcg tcctccgcaa cggcgcagcc
      121 gacgagcccg acggcgaagg cggagccacg gtgctcgccg cccgcgccct gaccgagggg
      181 acggagcgat acgacgcgat cgcgctggtc gaggcggggg agcggctcgg agccgccctc
      241 catgccgacg ccggctggga cgcgatgtcg atcggtgtgg atgttcccgc cgcccggctc
      301 gaacccgccc tcgagctcct tgccgaggtc gctctccacc cgacctttcc ggatgccgag
      361 gtcgatcgcc tccgggacga gcgcctcaac gacctgctcc aggccgaggc cgatccgcgc
      421 cgccgcgccg acgaggtgtt cgcggccacg atctacagcg gtggctcccc ctaccggcgg
      481 ccgtcgggtg gcctccgggc cacggtcgag acgctcgacg ccgcgcgtct ccgggcggcc
      541 ttccagcgcg ggctcgaccc cgcgcgagcg acgctcatcg tcggcggcga cctcggcggg
      601 atcgatgtgc gcgcgatcgc cgagcgcctg ctgggtggct ggggtgcggc ctacggcgcc
      661 gggccgaccg gctcgatcgt cgccgagagc gcggtccgtg aacggttcgt ccgggtcatc
      721 caccggccgg gctcggtcca gaccgagatc cggatcggtc acgtcggcct gccgcgacgg
      781 atcagcgatt tccacgccct gtccgtgatg ggcgcgatcc tcggcgggct gttcaactcg
      841 cggctgaaca cgaagctccg cgaggagaag ggctacacgt acggggccgg cgccgggttc
      901 gacctccgtc gcgcggccgg gccgttcgcc gcccgggctg cggtcaacac cgacgtcacg
      961 gttcccgcga tcgtcgacat gctggccgag ctcgaccgga tcaagacggc cccggtcgat
     1021 gcgtcggagc tcaaggcagc gcgtgacttc ctcgtcgggg tcttcccgct ccggttcgag
     1081 accccgggac cggtcgtcgg ggccctcgcc ggcctcgccg tccacgatct ttccgatgac
     1141 gagctgaccc gctatcgccc ggcgatcgag tcggtcacga tcgaggcggt ccttgccgct
     1201 gcgggccaca tccggaccga ccaggccgcc atcgtcctcg tcggcgatgc ggacgcgttc
     1261 ggcgccgatc tcgaggccgc ggggttcggg ccggtgcaga tcgagcgaga ccacggtccg
     1321 caggacgagg gccgggcgac cggcgtccag ggcgatctgg aaccgcttga cgaagggccg
     1381 gcgggtccga ccgagggtgg cgaggagccg gacaccggtt cggtggccgc ggagcctgca
     1441 gtcgactgac cgtcgggcgg cccgacgagc cgtccggtcg cgctgaaacc tttcggctcg
     1501 ctcgtgtctc agggaaacag atggccggcg cccaagtcgg tcgccgtctc gggagatcga
     1561 tcgaccatgg acgtcaagaa ccgactcgtg cgcggcgccg cgacctccgc cgtcgccctg
     1621 ttcctcgtca gcagcgccgt gctcggcgcg aacgcgatcg ccagcgcacc gcgcgtggtc
     1681 gacgcgaccc tgacgacgag cgaccagtcc gccgagcccg ccgagagcgc cgagccgacc
     1741 gaggccgccg agagcgccga gccgaccgag gccgcggaga ccgccgagcc gacggacacc
     1801 gccgagccga acgagccgac ggacaccgcc gagccgaacg aggacaccca cagtggccag
     1861 ggtggccagg tgagccagga cggcaacgac cagggcgagc atgggaccgc cgacccgacg
     1921 tcgaccgcgg gccacgatgg cgacaacgaa gatgcgagcg acaacgaaga tgcgaacgag
     1981 acggccgagc cggcggagac gcccgagccg accgatgaca acggcggcga ctcctcgggt
     2041 agcggatccg acagctccgg ccacgacggc agcggccacg actgagcccg tctcggcccg
     2101 tactggcagc gccgtcgcac ggaacgatgg accatggacg cataccttca gggccgccca
     2161 tggccgcatc gctccccagg ggtcccgtca cgccagcatc aaccgatcca ccaccattcc
     2221 acccagccga cgatcccgag gtcatcgatg aacgcgccgc tgtcgacgcc gtcctggccg
     2281 gcgaccgcga cgcgttccga tccctcgtcg atcgtgaatc ggccgcggtc atccgcgcct
     2341 gtcatcgcgt cctcggcgac atccacgagg ccgaggatgc agcccaggaa gccttcgtga
     2401 tcgcgtttcg cagcctcgcc ggctggcgcg ccgacggtcc gttcggggcg tggttgacca
     2461 ggatcgccgt ccggattgcc ctgcgccggg ctcagaagcg gcgcgacgtt tcgtggatcg
     2521 atccgaccca tcccgatcgc ccgctcgatc tgcccggtgg catcgacccg gcgacgagct
     2581 cacttcgggc ggagcgcgcc gccgatgtcc gggctgccgt cgctcgtctc gacgagccgt
     2641 accgcgagac ggtcgccctg cggttcttcg gcgatctcag cctcgacgag atcgcgaccc
     2701 agaccggccg cccgctcgga accgtcaaga cccatctcca tcgcgggctc attcgcctgc
     2761 gcggcgtcgt cgagacgggt gcgctgtgaa cggcgagctg ccgctccatc cgtccgacga
     2821 agagcgcttg gccgcggcca tggcccgcga gctggagtca atcacgacga tgacgcccgt
     2881 ccagccgggc gagggatttg ccgatcgagt catgctcgcc atcgccgacg agccgctgcc
     2941 gcagccggtc cgggcgttcg gcctggccct gctcgggggt cacctccggg ccgccgcatc
     3001 cgccgtcggc gatgcgtggc ggacgatcgc gagctcacca gcgccgttgt tcgtccgcgc
     3061 ccaggcgctg gctctcgtcc tcgtcgtgct ggtcgcgtcc cttgcgctgg ccggcggggc
     3121 cacggtcgga gcgatgggtc tgctcggcaa tgggccagtg acgccagttc cttccggacc
     3181 gccaccgacc gtgcctgtcg ccagcccgac tccctcgccg ttgccacagg ccgaggacac
     3241 gccgagcgcg tcaccggagg agtcgagcga tgcagaggcg accgagacgc ccgacgcgac
     3301 cgagacgccg gattcacagg aaacggagcg ggcgaccacg cgaccccgga cagccgagcc
     3361 gaccgaagac cacggcggcg atcggtccgg gtcagaatcc ggctcaggat ccggctccga
     3421 cggaggggac cacaccccga gcccgaccga gaccgacaac cactcgggcg gcgacggcta
     3481 agtgaagttc cccggtgaag atgagactgg gtaccgtggc cctcgaggag ggagcggatg
     3541 ccggagttca agtctcggac gtacgccaac ccgcggcgcc tgccgcggcg gaaccactac
     3601 caggagggcc cggacttcct gttcgtcgcg acccagggcg ggcgaccacc ggagagcacc
     3661 gtcggagacg gaccggcggc gagcgtccgg cgccgtcgtg ccgcgctccg acgctggggc
     3721 gacggggagc cgatcggtgc gatctgcgcg gaggttcact gcagccgggc gagcctgttc
     3781 cgctggcgga cgcgctacga ggcgggcggc ctcgaggcgc tcgtcgatcg accgcgggtc
     3841 ggccgcgcga gcgccctgcc gccagccatc gagcggctca tcctgaccgt ccgcctgctg
     3901 acctactgga acagtcggcg gatcgccgcc gagttccgga ggcgtggggt ctggccgctc
     3961 ggccatggcc agatcgaccg gctcctcgac cggaacggca gccatcggcc gagctatgtc
     4021 cggacacccg gcccgcgcta cgagcgggcc gccgtcaacg agctctggca catcgacctc
     4081 aagggcccgt tctacctgct cggcgcgacc ggccgagcgc ggacctgtca cttcgccgcc
     4141 ctggtcgacg accacagtcg gttcctgctc gggatccggg ccgtgccgac caaggaggcg
     4201 gtctggatcc tcgccctgct cgaggaggcg atcgagctgt gcggcgtccc gcaggagctg
     4261 atgaccgaca acgggacacc cttcgtggcg atcgtccgga cgatgctcag ccgcttccag
     4321 cggagcctgg ccgagctcgc catccggcac atccggaccc agatcgacac gccctggacg
     4381 aacggcaaga tcgagcgctt ctgggcgact ctccaggccg aggtcctcga ccgtcagcag
     4441 ctcgtcgacc tggctgaggc cgaggccgcc gtcaccgcct acgccggcta ctacaactac
     4501 caccggcttc acggcgagct cgactggcag acgcccgccg aacgcttcga cggcacgccg
     4561 ttcaccgacc ggggcttcgg gagcgtgcct gccctggccg gtgtcgccga tctcctcgac
     4621 gcatccattc gtccggacct cggtcaagcc cacgccttct gcggccactg tggcccccga
     4681 tcgccagcca gcgcctct
//
LOCUS       DATAIW010000172        15056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_98121, whole genome shotgun sequence.
ACCESSION   DATAIW010000172 DATAIW010000000
VERSION     DATAIW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98121"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1452
                     /locus_tag="VHM48_05520"
     CDS             238..1452
                     /locus_tag="VHM48_05520"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2754899.1"
                     /translation="MNRYAGLDWASEIHAVCVIDETGEVLERLRIPHTRLGLAELTSR
                     LARHADPAELPVGVERSDGPLIEALRAGGFPVVVIPPHTVKAARPRFSAANVKSDPGD
                     ARLLADLVRTDGGRFRVLTPSDPATAALRRLVRTRGELVAERTRLANRLRGLLEDSWP
                     GAAEIFARVDSPIGLDFCERYPSPRSARTLGEARLVSFLVRHHYSGRRPAGELLARLR
                     AAPVVTEPAIVDEATADAVRALVACLRAILARLADLEGAIRAALAAHPDGALFRSFPR
                     AGEISAAMLLAEIGVDRERFPSAEALAAQAGAAPVTRASGKSSVVGFRWACDHRLRQA
                     LTQFADNSRHASPWADDVYRRARRRGADHPHAIRILAKAWSRVIWRCWQDRAPYDPAR
                     HGGLNRLEQTAA"
     gene            complement(1476..2153)
                     /locus_tag="VHM48_05525"
     CDS             complement(1476..2153)
                     /locus_tag="VHM48_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2754900.1"
                     /translation="MPRRLLVAVRDPVLLGRLESLAARHRLHIHSADDGAAPPDEEPP
                     VAVIAELELAGAVDRIVEWRRRWPDTVLAGSIRIPDQELWHGAIAAGADLVANRGAFV
                     PQLERLLDERLAARGPAASASRLAVRLIEREGDGLVGRLPDAPGGSIAIFRVGDRLHA
                     VRDECPHAGASLADGELVGPIITCPRHGSQFDVTSGSRERGPSDYPIRTYRVVVDGDR
                     ILVEVQA"
     gene            complement(2163..2579)
                     /locus_tag="VHM48_05530"
     CDS             complement(2163..2579)
                     /locus_tag="VHM48_05530"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2754901.1"
                     /translation="MDDAIPRLRAIDAGVLERASRVIRVVGHPLRLRLLELLEGGERN
                     VTDLVQTTGVGQAMVSQQLKILRAEGVVGDRREGPRVFYRITEPKVSRILDCIRECDL
                     PDLPGPSDVAGGLDLRVLPSSSRPSGASRRERPTTG"
     gene            2841..3080
                     /locus_tag="VHM48_05535"
     CDS             2841..3080
                     /locus_tag="VHM48_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2754902.1"
                     /translation="MTEARTADQTLDCKGLLCPMPIVKLAKAFKELVPGQVLLLEATD
                     PGSVPDVAAWSKNTGNPILSQDRDGAVMRFWIEKA"
     gene            3116..3487
                     /locus_tag="VHM48_05540"
     CDS             3116..3487
                     /locus_tag="VHM48_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2754903.1"
                     /translation="MASILYVHTGGVANPERSATPIYLATTAAAMDIEVGIYFTVNGP
                     TLLKKGAAEALIVPKYDGNGAPLKRFIDQAIDMGVKFYVCQPSLDLHGLTVDDLIDGV
                     KMIGGAAFNDMALEADRVICF"
     gene            3498..4187
                     /locus_tag="VHM48_05545"
     CDS             3498..4187
                     /locus_tag="VHM48_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2754904.1"
                     /translation="MTLEARYADVPMETKQQLFDEVKADRRFKDYLYGCYECGICVAA
                     CPSARFYDFSPRKIAQAASREDLPMFFEQLNDDIWNCSQCFSCTRCPRGNNPGGLITI
                     MREVSINNGLTSAKQALEGYGRVVYKIMSTGTQVSPDMLQPNAFPDWGPHVQEISDNL
                     ALWRRALPPDTMHTTSTAWEVDDKTLTELYLIWHMTGVLDMIKGVDPGLHMILNDVME
                     ELLEEAGYSAD"
     gene            4214..5395
                     /locus_tag="VHM48_05550"
     CDS             4214..5395
                     /locus_tag="VHM48_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX2754905.1"
                     /translation="MTVLDTRREARDERGPATPPTEDVRERVFELEARGEWLVQRVPE
                     PWIGVTTKYGRTKKIPIAKTWHHKSCGQCGHIPGYSTSIFWLIRKLGLDYSDPKDQTS
                     CTAWNYYASATSNQGAQAAVAMRNFGAAYESGYFPMIHCGTSYGHYKEVREELVHSAD
                     LRDEVRRICDKLGKPFVMPEEIVHYSEWVYAMRDEFAKRRVLDLSAIDVTVHPACHYH
                     KLVVEDAIYDPDVYGGQRTAVVSAVVTALGANVKDYSTWFDCCGFGFRHILVQRDFTR
                     SFATMRKIEVMREEANPDVVLTHDTGCVTTLDQSQFAARAHKPNVGIPVMSEAQFAAL
                     AMGAHPYRVAQLHWHSTEYRPLLEKMGIDWRERWREFEDDLERLKKNDKTYLTWEDAD
                     V"
     gene            5388..6455
                     /locus_tag="VHM48_05555"
     CDS             5388..6455
                     /locus_tag="VHM48_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2754906.1"
                     /translation="MSDTVLVVGGGPAGLEAAHAIADMSARAILVERRAVLGGTPDES
                     SYAALTPRMGSAQEALDLLRAGVQDPNVEIHLETQLTACSGSVGNFSVTLEGPAGVTT
                     ETVGSIVVATGFQHFDPGRETQMYGYYEYPDVITLPDAEKMLSEHRFVRPSNGEPPRN
                     VAFIQCVGSRDRRLGNEYCSKVCCGVASKEAIEIRRLLPDAKVYIFYIDMRMYGYWEN
                     EIYWPAQEQYNVNYIRGIVTEIVARDGRLLMKGEDTTMGRPLEVLMDVIVLSVGMEPS
                     RGTRDVAGILGVDQNKYGFIATPGDPMDPVGTSVPGIFVAGAAAGPKDLEDTMSMAGA
                     AAMRALRAIRGQARAEVGATR"
     gene            6452..7246
                     /locus_tag="VHM48_05560"
     CDS             6452..7246
                     /locus_tag="VHM48_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754907.1"
                     /translation="MTWGPAVFGGGTGIVDRDSVDSPSAVEFMREALIKISEEDIGAV
                     AELLARKHRALAPVLGADALASANAEAVRPILARTFATRRRTDEILVKVGAPVLATAI
                     RDLLHGGGGIDARFRAFDEALEPIEAPVRRDLAGECLHFLDPERYWLWSRWMWDPQTM
                     TGALPLVMVDDFDFDGPDAGAVYLRVGAATAAVIATADELGFKRMRASPFVVEVYLAA
                     IYGIYLYTVTRLRMTQEFNRVLPQLPDLVRRLLGVRRLDEAVGHGA"
     gene            7254..8537
                     /locus_tag="VHM48_05565"
     CDS             7254..8537
                     /locus_tag="VHM48_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2754908.1"
                     /translation="MMKVDRVIELEHAVVDGVDISGHWNRMFTDRSIEEYPTEAMDWV
                     TGLAGGESLRSCYQCGKCVPVCPVNHVGSYGPRKLHVKVLRGNNLLDDPDLWLCTTCG
                     NCLRVCPKEVNPLEFMPAIREQAVLEGNVPAELAKVFENLQRQGNPHGHSPRKRADWV
                     ADAGVPVPLIAEIGRPVDVLWYVSDYTSYHPRGQDAARAMARILTALGIDFAILGNEE
                     RSDADSARLAGETGLFEMLAEQNIKTFRKYEFERLVVMDPHAFNAFKNVYPRHGWEGE
                     VLHYTQFLAPLIDRIEFKSSVDVDVTYHDPCYLGRNNGEFDAPRRLLEAIPGVRLAEM
                     PHNKKNGYCCGGGGGGMWLDGFVADHVSERLSERRVREAVETVAMDGTGVLSVACPYE
                     PSRFEDAVKSTGNDGRLIVRDIVELIDQAMQPATS"
     gene            8616..9395
                     /locus_tag="VHM48_05570"
     CDS             8616..9395
                     /locus_tag="VHM48_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754909.1"
                     /translation="MELIVLLKAVPDLTEELEIDAAGTGLATEWLTWVASEWDEYAVE
                     EALQLKESFGATVTCLALDTGSVDELLATCLARGADRVLKVGSFERPPDSHTAARAFA
                     AALGGLSYDLVLTGVQAIDDVDGQVGPLLGAALDLPHVSVVTHIALATDGKSATVNQE
                     YAGGIVGEFEVDLPAILGVQTSREVPRYAPVSRVRQLLKTAEIGHLDAPADGGSGLIV
                     EAMEVPKADHQAEMIGGNADAIAARLVEILRERGLTSGSAR"
     gene            9392..10354
                     /locus_tag="VHM48_05575"
     CDS             9392..10354
                     /locus_tag="VHM48_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2754910.1"
                     /translation="MSGTILVLVEHRDGVVADATFELLGRARTLASAASVEVEAVLLG
                     SGVDDLVGQLGAADRVVLIDDPALAEFTPSGYHSALVPVVRDRAPLLVLAANTGVGMD
                     VAAGLSMATGLALAAYAIDVTVAGGSPLVTCQLYAGKINARVRFADGRGIVTLVVGTA
                     PAEPGNVAGSPPIERIGVAASDGRIRFRRFIRPEAGDVDITRESILVSVGRGIGEREN
                     LELVEELATVLGGVVSSSRPIADAGWLPRTRQVGKSGQRVKPRLYLALGISGAPEHLE
                     GMRGADLIIAVNTDPRAPIYGVANYGAVADVIDVVPALTKQLRG"
     gene            10362..12638
                     /locus_tag="VHM48_05580"
     CDS             10362..12638
                     /locus_tag="VHM48_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX2754911.1"
                     /translation="MLDASVTREVFEGLSPDGELAFYLLAALATTIFVVGLGLRLRKY
                     LRGRREDRVGSLTAFIGRAIGGVRATATSETVRKRDAYAGIFHAAVMWGFIVLFIGTV
                     ILTIDTDIVGLIAPQYHFFNGAFYVVYSFILDLLGLALLVGLAALAWRRARFHKRELD
                     YARVDLGAATTDRRPFVFGDWLFLGWLGLLGTSGFIVEGVRIVAHDFPWFEAFSPVGE
                     ILARVVAFLGLSAATAGSLHPWLWWFHAIAALGFVAYIPYAKAIHMLTDGANLALRSP
                     LAGRRLPVIDPAPRPLLAGLAPAAAGDSHIGIRDLADFSWKQLLDFDACTKCGRCHAA
                     CPAVAAGAPLSPRDLILDLRQQADASWAVRGPGMERRSDVTYGGARADAGEPVFSDVA
                     TPVTRGGLMAGGLVKAETLWACTTCLACVEACPVGIEHVPAIVGMRRSLVDQGAVEPG
                     LQTVLTSLAKQGNSFSQSGRGRAKWADGLPTPLRDARREEVDWLWFVGDFASFDVRVQ
                     EATRLVARVLQAAGVDFGILFEGERNAGNDVRRVGEEGLFEILVEHNVGQLEKARFRR
                     IVTTDPHTLNTLRHEYPDFGGRYEVWHYSQLLAALFDDGLLRTRRPIARRATYHDPCY
                     LARYNDVTRAPRDVLAAIGARLVEMPRNGTNTFCCGAGGGRIWMDDSGLRERPSEQRM
                     REAAALERVTDFVTACPKDLTMYTAAAKATGLDDRLVVSDLIELVARAIDLGDEVAEE
                     SSMDDEVALLAAAGSAGA"
     gene            12649..13179
                     /locus_tag="VHM48_05585"
     CDS             12649..13179
                     /locus_tag="VHM48_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754912.1"
                     /translation="MPDVTDAAVGAVAADAPVVADELLDRLAAAVAARVRGRRTAEER
                     LGAFAFHEGPRHGRQAGMAPLDRSTADESTPDSAAIDWRYFVLRALTALGDPDAVRIL
                     EALPPDGRPLDALVGLVEPDVPDPVAAADRIGRLAAAGLVGRDLESDHVSLLPLGEVL
                     LELVRDVARRASVADR"
     gene            13212..13505
                     /locus_tag="VHM48_05590"
     CDS             13212..13505
                     /locus_tag="VHM48_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754913.1"
                     /translation="METHAHLGDVHGDQVRTFRDEQRDKLMFALDCPFCDEGMERVAN
                     PRGREAGFLDEFRREITLVAFDLLLYHFQASHAERVQLPPVPADTGPETGGTG"
     gene            13502..13939
                     /gene="gcvH"
                     /locus_tag="VHM48_05595"
     CDS             13502..13939
                     /gene="gcvH"
                     /locus_tag="VHM48_05595"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2754914.1"
                     /translation="MTQVRGCEVPEDLYYWVEKHVWARHDGDGLLTIGVTDTAQHLAA
                     RVVAITAKKVGRQIERGQSVATLESGKWVGPVPAPVDGEIAEVNPAIATDPTIVNSDP
                     YGAGWIVKFRATDWSGQHAQLVTGAAGVEAYRAFLEAEGIACD"
     gene            13932..14408
                     /locus_tag="VHM48_05600"
     CDS             13932..14408
                     /locus_tag="VHM48_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2754915.1"
                     /translation="MIEGLPGHAGSYRGCEIPPDLLYDIERDVWVRPGPDRTLTMGMT
                     DPAQSRAGKFLHIQFKKVGRHLEAGQSAATIETAKWAGPFPSPVAGTIVDTNEAAYGA
                     DILIANRDPYGAGWIVRLAPDDPTAAISNLMTGPEAVASYEARIDLLGLNCIRCAD"
     gene            14421..>15056
                     /locus_tag="VHM48_05605"
     CDS             14421..>15056
                     /locus_tag="VHM48_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX2754916.1"
                     /translation="MNAIRLLDLGSVPAIRSQTIYHAVGYALGPETPDTIILVSPDAP
                     YVSIGRHQDADREVDLDYCREAGLPVIRREVGGGAVYLDGNQVFTQWAFRADHLPATV
                     EDRFRLYVETLVRAYRTLGIAAEYRPINDVHVAGRKIGGTGAARMDRAEVVVGSLMFD
                     FDMATMARVLKVPSEKFRDKVHQSLIDYMTTMRRELGEPPPRQVVVDAYVAA"
BASE COUNT         2293 a         5347 c         5120 g         2296 t
ORIGIN      
        1 cgctcattgg agcaccctct caactgccac gatgagggtg gccctcgggt cctgccgggg
       61 tgacgagtcc gctcgcttgt cgcgtgacga ccagcatgag atctctcgcc cccggtggcc
      121 cgggtgaccc ggatcggcca cagggaatag cgccacagag cgccgagcaa tgagcgaccg
      181 accagggttc cccaggactc cgggggcgtg accatcatcg caccggagga cgatccgatg
      241 aaccgatacg ccgggctcga ctgggcgtcc gagatccacg ccgtctgcgt tatcgacgag
      301 accggcgagg tcctcgagcg gctgcggatc ccgcacactc ggctcggcct ggccgagctc
      361 accagccggc tcgcccggca cgccgacccg gcggagctgc cggtgggggt cgagcgctcg
      421 gacggcccgc tcatcgaggc gctccgggcc ggcggcttcc cggtcgtggt catcccgccc
      481 cacacggtca aggcggctcg cccgcgcttc agcgcagcca acgtcaagag cgacccgggc
      541 gatgcgcgcc tgctggccga cctcgttcgg accgatggcg gccggttccg ggtgctcacg
      601 ccgtccgatc ccgcgaccgc cgccctgcgc cggctcgtcc ggacgcgtgg cgagctggtc
      661 gccgaacgga cccggctcgc caaccgcctg cgtggcctgc tcgaggacag ctggccgggc
      721 gcagccgaga tcttcgcccg ggtcgactcg ccgatcggac tcgacttctg tgagcgctat
      781 ccgagcccgc gctcggcccg gacgctcggc gaggcgcgtc tcgtgtcgtt cctcgtccgc
      841 caccactact ccggccgacg gccggccggc gagctcctgg cgcgcttgcg ggccgctccg
      901 gtggtgaccg agccggcgat cgtcgacgag gccacggccg atgccgtccg ggcgctcgtc
      961 gcctgcctgc gcgcgatcct cgcccggctg gccgacctgg aaggcgcgat ccgggcggcg
     1021 ctcgcggccc atcccgatgg agcgctcttt cggagcttcc cgcgggccgg cgagatcagc
     1081 gctgccatgc tcctcgccga gatcggagtc gatcgcgagc ggttcccgtc ggccgaggcg
     1141 ctggccgccc aggcgggtgc cgcgcccgtc acccgagcgt ccggcaagag ctcggtcgtc
     1201 ggcttccgct gggcctgcga ccatcggctc cggcaggcgc tcacccagtt cgccgacaac
     1261 agccgacacg ccagcccgtg ggccgacgac gtgtaccgcc gggcgcgccg gcgtggggcc
     1321 gaccacccgc atgcgatccg gatcctggcc aaggcctggt cgcgggtcat ttggcgctgc
     1381 tggcaggatc gcgcgccata cgatccggct cgccacggcg gcctcaaccg tctcgagcag
     1441 acggccgcct gacaggaggg gttgacacag ggatctcatg cctggacctc gacgaggatc
     1501 cggtctccgt ccacgacgac gcggtacgtg cgaattggat agtcggacgg cccccgctcg
     1561 cgtgatccgc tggtgacatc gaactggctg ccgtgcctgg ggcaggtgat gatcggtccg
     1621 accagctccc cgtcagccag ggatgcaccc gcatgggggc attcgtcgcg gacggcatgg
     1681 agccggtcac cgacgcgaaa gatggcgatc gagcctccgg gcgcgtcggg caaccgcccg
     1741 accagaccgt cgccttcacg ctcgatgagc ctgaccgcaa gtcggctggc gctcgccgcc
     1801 ggtccgcgag cggcgaggcg ctcgtcgagc aggcgttcga gctgtggcac gaaagccccc
     1861 cggttggcga cgaggtccgc gccggccgcg atcgcgccgt gccacagctc ctggtccggg
     1921 atgcgaatgg atcccgccag gacggtgtcc ggccagcgcc tccgccattc gacgatccga
     1981 tccacggcgc ccgccagctc gagctccgcg atcaccgcga cgggcggctc ctcatcgggt
     2041 ggtgcggcgc cgtcgtcggc cgaatggatg tggaggcggt gcctcgccgc cagtgattcg
     2101 aggcggccca gcaggaccgg gtcgcgcacg gccacgagca ggcgacgtgg catcgcaagc
     2161 tcctagcccg tcgttgggcg ttctcgtcgc gacgcgcctg acggtcggga cgacgacggc
     2221 aggacccgca ggtcgagtcc gccggcaaca tccgacggcc ccggaaggtc agggaggtcg
     2281 cactcgcgga tgcagtcgag gatccgcgag accttcggct cggtgatccg atagaagacc
     2341 cgcgggcctt cgcgacgatc gccgacgacg ccttcggccc gcaggatctt caactgctgg
     2401 ctgaccatcg cctggccgac gccggtggtc tggacgagat cggtcacgtt ccgctcaccg
     2461 ccctcgagca gctcgaggag ccggagacgg aggggatggc cgaccacccg gatgacccgt
     2521 gatgcacgtt cgagaacgcc cgcgtcgatg gcccggaggc gaggaatggc gtcgtccatg
     2581 gccggcacag tctacgtgag cccgaggcgt cgggccgccg ctcgtccttc gcgatcaggc
     2641 gcgaatcgag tccgcgatga acggccgaat cgggtgtcag gacggttcaa cgacggcctc
     2701 cggcatgcga attgacatgc cctgtggcgc ggagtatata gggacatcac gatacggcaa
     2761 tagccacgat cggcattccg gcgtcggtgc gcgtttcggg gactgcagat cgccctcgca
     2821 cccggtcacg gagggtcacg atgacggaag cccggacggc ggaccagacg ctcgactgca
     2881 agggcctgct ctgcccgatg ccgatcgtca agctggcgaa ggcgttcaag gagctcgtgc
     2941 cgggccaggt cctcctgctc gaggcgaccg acccggggtc cgtcccggac gttgccgcct
     3001 ggtcgaagaa cacgggcaat ccgatcctgt cgcaggaccg ggacggggcc gtcatgcgct
     3061 tctggatcga gaaggcatga ccgctggtcg atcgcgctag gcaggggcga tcgcggtggc
     3121 cagcatcctc tacgtccaca ccggcggtgt cgccaacccc gagcggagcg cgaccccgat
     3181 ctacctcgcg acgacggcgg cggccatgga catcgaggtc ggcatctact tcaccgtcaa
     3241 cgggccgacc ctcctgaaga agggcgcggc cgaggcgctc atcgtcccca agtacgacgg
     3301 caacggcgcg ccgctcaagc ggttcatcga ccaggcgatc gacatgggcg tgaagttcta
     3361 tgtctgccag ccgagcctcg acctccacgg gctgaccgtc gacgacctga tcgacggggt
     3421 gaagatgatc ggcggggcag cgttcaacga catggccctc gaggccgacc gggtcatctg
     3481 tttctaggga gcgccggatg actctcgagg ctcgctacgc cgacgtccct atggagacca
     3541 agcagcagct gttcgacgaa gtcaaggccg accggcgctt caaggactac ctctacggct
     3601 gctacgagtg cgggatctgc gtggccgcct gcccgtcggc ccggttctac gacttctcgc
     3661 cgcggaagat cgcccaggcg gccagccgcg aagacctgcc gatgttcttc gagcagctca
     3721 acgacgacat ctggaactgt tcgcagtgct tctcgtgcac ccgctgtccg cgcggcaaca
     3781 acccgggcgg tctgatcacg atcatgcgcg aggtctcgat caacaacggc ctgacgtcgg
     3841 ccaaacaggc cctcgagggc tacggccggg tcgtctacaa gatcatgtcg accggaaccc
     3901 aggtctcgcc ggacatgctc cagccaaacg cgttccccga ctgggggccg cacgtccagg
     3961 agatctcgga caacctcgcg ttgtggcggc gggcccttcc gccggacacg atgcacacga
     4021 catcgacggc ctgggaggtg gatgacaaga ccctcaccga gctctacctg atctggcaca
     4081 tgacgggcgt gctcgacatg atcaaggggg tcgaccctgg tctccacatg attctcaacg
     4141 acgtcatgga agagctgctc gaagaggccg gctactccgc cgactgagga ggtccacccg
     4201 tatgccgatc ccgttgaccg ttctggacac gaggcgcgag gcgcgcgacg agcgtggtcc
     4261 ggcgaccccg cccaccgagg acgtccggga gcgcgtgttc gagctcgagg ctcgcggtga
     4321 gtggctcgtc cagcgcgttc ccgagccgtg gatcggggtg acgacgaagt acggccggac
     4381 caagaagatc ccgatcgcga agacctggca ccacaagtcg tgcggccagt gcggccacat
     4441 cccgggctac tccacgtcga tcttctggct catccgcaag ctcggtctcg actacagcga
     4501 ccccaaggac cagacgtcct gcaccgcctg gaactactac gcctcggcga cctcgaacca
     4561 gggcgcccag gccgcggtcg cgatgcgcaa cttcggggcg gcctacgagt ccggctactt
     4621 cccgatgatc cattgtggga cctcgtacgg ccactacaag gaggtccgcg aggagctcgt
     4681 ccactcggcc gacctgcgcg acgaggtccg gagaatctgc gacaagctcg gcaagccgtt
     4741 cgtcatgccg gaggagatcg tccactactc cgaatgggtg tacgcgatgc gcgacgagtt
     4801 cgccaagcgc cgcgtcctcg acctgtcggc aatcgacgtg acggtccacc ccgcctgcca
     4861 ctaccacaag ctcgtcgtcg aggacgcgat ctacgacccc gacgtctacg gcggccagcg
     4921 gaccgccgtc gtgtcggccg tcgtcaccgc cctcggtgcg aacgtcaagg actacagcac
     4981 ctggttcgac tgctgcggct tcggcttccg ccacatcctg gtgcagcgcg acttcacccg
     5041 gtcgtttgcg accatgcgca agatcgaagt catgcgcgag gaggccaacc cggacgtcgt
     5101 gctgacccac gacacgggtt gcgtgacgac gctcgaccag agtcagttcg cggcccgcgc
     5161 ccacaagccg aacgtcggca tcccggtcat gtcggaggcg cagttcgcgg ccctcgcgat
     5221 gggcgcccac ccataccggg tcgcccagct ccactggcac agcacggagt accgtccgct
     5281 gctcgagaag atgggcatcg actggcgaga gcgctggcgg gagttcgagg acgacctcga
     5341 acggctcaag aagaacgaca agacatacct cacctgggaa gacgccgatg tctgacaccg
     5401 tgctcgtggt cggcggcggg ccggccggtc tcgaagccgc ccatgcgatc gccgacatga
     5461 gcgctcgcgc gatcctcgtc gaacgccggg cggtcctcgg tggcaccccc gacgagtcga
     5521 gctatgccgc tctcacgcca cgaatgggct ccgctcagga ggcgctcgac ctcctccgcg
     5581 ccggcgtcca ggatcccaac gtggagatcc atctcgagac ccagctcacc gcctgcagcg
     5641 gttcggtcgg gaacttctcc gtgaccctcg aggggccggc cggcgtgacg accgagacgg
     5701 tcggatcgat cgtcgtcgcg actggcttcc agcacttcga tccgggtcgc gaaacccaga
     5761 tgtacggcta ttacgagtac cccgacgtca tcaccctgcc cgacgccgag aagatgctca
     5821 gcgagcaccg cttcgtccga ccgtcgaacg gtgagccgcc ccgcaacgtc gcattcatcc
     5881 agtgcgtcgg ctcgcgcgat cggcgcctcg gcaacgagta ctgctcgaag gtctgctgcg
     5941 gggtcgcctc gaaggaggcg atcgagatcc ggcggctcct gccggacgcc aaggtctaca
     6001 tcttctacat cgacatgcgg atgtacggct actgggagaa cgagatctac tggccggccc
     6061 aggagcagta caacgtcaac tacatccgcg ggatcgtgac cgagatcgtc gccagggacg
     6121 gccggctcct gatgaagggc gaggacacca cgatgggccg gcccctcgaa gtcctgatgg
     6181 acgtcatcgt cctgtcggtc gggatggagc ccagccgggg gacccgcgat gtcgccggga
     6241 tcctcggggt cgaccagaac aagtacggct tcatcgccac cccgggtgat ccgatggacc
     6301 cggtcgggac aagcgttccc gggatcttcg tcgcgggcgc agccgccggg ccgaaggatc
     6361 tcgaggacac gatgtcgatg gccggcgcgg cggccatgcg ggccctccgg gcgattcgag
     6421 gtcaagcccg ggccgaagtc ggcgcgacgc gatgacctgg gggcccgccg tgttcggcgg
     6481 cggcacgggc atcgtcgacc gcgattcggt cgattcgccg agcgccgtcg agttcatgcg
     6541 cgaggcgttg atcaagatca gcgaggagga catcggcgcc gtcgccgagc tccttgcccg
     6601 caagcaccgc gccctcgccc ccgtcctcgg cgcggatgcg ctcgcctcgg ccaacgccga
     6661 ggccgtccgc ccgatcctcg cccggacgtt cgccacccgt cgccgtaccg acgagatcct
     6721 cgtcaaggtc ggagcgcccg tcctcgcgac ggcgatccgc gacctgctcc acggcggcgg
     6781 ggggatcgat gcccggttcc gcgccttcga cgaggcgctc gagccgatcg aggcgccggt
     6841 ccggcgcgat ctcgcggggg agtgcctcca cttcctcgac cccgagcgct attggctgtg
     6901 gagtcgctgg atgtgggacc cccagacgat gaccggggct ctccccctcg tcatggtcga
     6961 cgacttcgac ttcgacggcc ccgatgccgg cgccgtctat ctgcgggtcg gcgcggcgac
     7021 cgcggcggtc atcgccaccg ccgacgagct cggcttcaag cggatgcggg cgagtccgtt
     7081 cgttgtcgag gtctatctcg cggcgatcta cgggatctac ctgtacacgg tgacccggct
     7141 gcggatgacc caggagttca atcgcgtcct cccccagcta ccggatctcg tccggcggct
     7201 gctcggggtc cgtcgactcg acgaggcggt cggccatggg gcgtgagctg ccgatgatga
     7261 aggtcgaccg cgtcatcgaa ttggagcatg ccgtcgtcga cggcgtcgac atctccggtc
     7321 actggaaccg gatgttcacc gatcgctcga tcgaggagta cccgaccgag gcgatggact
     7381 gggtgaccgg cctcgccggc ggcgaatcgc tgcggagctg ctaccagtgc ggcaagtgcg
     7441 tccccgtctg cccggtcaat cacgtcggca gctacggccc gcgcaaactc cacgtgaagg
     7501 tcctccgtgg caacaacctg ctcgacgacc cggacctctg gctgtgcacg acgtgcggca
     7561 actgcttgcg cgtctgtccg aaggaggtca acccgctcga gttcatgccg gcgatccggg
     7621 agcaggccgt gctcgagggc aacgtcccgg ccgagctcgc gaaggtgttc gagaacctcc
     7681 agcgccaggg caacccgcac ggccattcgc ctcgcaagcg ggccgactgg gtcgccgacg
     7741 cgggcgtccc ggttcccctc atcgcggaga tcggccgtcc ggttgacgtc ctctggtacg
     7801 tctcggatta cacctcgtac cacccccgtg gccaggatgc cgcgcgggcc atggcccgga
     7861 tcctgacggc cctcgggatc gacttcgcga tcctcggcaa cgaggagcgg agcgacgcgg
     7921 attcggcccg gctggccggc gaaaccggat tgttcgagat gctcgccgag cagaacatca
     7981 agacgttccg caagtacgag ttcgagcgcc tcgtcgtcat ggacccgcac gccttcaatg
     8041 cgttcaagaa cgtctatccg cggcacggct gggagggcga ggtcctccac tacacccagt
     8101 tccttgcccc gctgatcgac cggatcgagt tcaagtcgag cgtcgatgtc gacgtgacgt
     8161 accacgaccc gtgctatctc ggccgcaaca acggcgagtt cgacgcgccg cgcaggctgc
     8221 tcgaggcaat ccccggcgtc cgcctggcag agatgccgca caacaagaag aacggctact
     8281 gctgcggcgg cggcggcggc gggatgtggc tcgacggctt cgtcgccgat cacgtctcgg
     8341 agcgcctgtc cgagcgccgc gtccgggaag ccgtcgagac ggttgccatg gacgggaccg
     8401 gcgtcctgtc ggtcgcctgt ccgtacgagc cgtcgcggtt cgaggacgcc gtcaagtcga
     8461 ccggcaacga cggccggctg atcgtccgcg acatcgtcga gctgatcgac caggcgatgc
     8521 aaccggcgac gtcctgaccc gatgggtcga cgacgcccgc gcgcatcgcg gggctcacac
     8581 cgcatccctg catacgcgct gttcgaggag gactgatgga gctgatcgtg ctgctcaagg
     8641 ccgtgcccga tctgaccgag gagctcgaga tcgacgccgc cgggacgggc ctggcgacgg
     8701 agtggttgac ctgggtcgcg agcgagtggg acgaatacgc ggtcgaggag gcgctccagc
     8761 tcaaggaatc gttcggggcg accgtcacct gccttgccct cgacacgggc tcggtcgacg
     8821 agctgctcgc gacctgcctg gcccgcggcg ccgatcgcgt cctcaaggtg ggttcgttcg
     8881 agcggccgcc ggactcgcac accgcagccc gggcgttcgc cgccgcgctc ggcggactct
     8941 cctacgacct ggtcctgacc ggcgtccagg cgatcgacga cgtcgacggt caggtcggcc
     9001 cgctcctcgg cgccgccctg gacctgcccc acgtcagcgt cgtgacccac atcgcccttg
     9061 ccacggatgg caagtcggcg acggtgaacc aggaatacgc cggcggcatc gtcggcgagt
     9121 tcgaggtcga cctgccggcg atcctgggcg tccagacctc gcgcgaggtt ccccgctacg
     9181 cacccgtcag ccgggttcga cagctgttga agacggctga gatcggccat ctcgacgcgc
     9241 ccgccgacgg cggctcgggg ctcatcgtcg aggcgatgga ggtcccgaag gcggaccacc
     9301 aggccgagat gatcggcggc aacgccgacg cgatcgcggc acgactggtc gagatcctcc
     9361 gtgagcgtgg actgacgtcc gggagcgccc gatgagcggc acgatcctcg tcctcgtcga
     9421 gcatcgcgat ggcgtcgtcg ccgacgcgac cttcgagctc ctcgggcggg cccgcacgct
     9481 ggccagcgcc gcgtctgtcg aggtcgaggc cgtcctcctc ggctccggcg tcgacgacct
     9541 tgtcggccag ctcggcgccg ccgaccgcgt cgtcctgatc gacgacccgg cgctggccga
     9601 gttcaccccg agcggttacc actcggcgct cgtcccggtc gtccgcgatc gggcgccgct
     9661 cctcgtcctg gccgcgaaca ccggcgtcgg gatggacgtc gcggccgggt tgtcgatggc
     9721 aacgggcctc gccctcgctg cctacgcgat cgatgtgacc gtcgccggcg gttcgccgct
     9781 cgtcacctgc cagctctacg ccggcaagat caacgcccgg gtccggttcg cggacggccg
     9841 ggggatcgtg acgctcgtcg tggggacggc gccggccgag cccggcaacg tcgccggctc
     9901 gccgccgatc gagcggatcg gggtcgcggc cagcgacggc cgtatccggt tccggcggtt
     9961 catccggccg gaggccggcg acgtcgacat cacgcgcgag tcgatcctcg tgtccgtcgg
    10021 gcgggggatc ggcgaacggg agaacctcga gctggtcgag gagctggcga cggtcctcgg
    10081 cggcgtcgtg tcctcgtcac ggccgatcgc cgacgccggc tggctgccaa ggacgcgcca
    10141 ggtcggcaag tccggccagc gggtcaagcc caggctgtac ctcgccctcg gcatcagcgg
    10201 ggcaccggaa cacctcgagg ggatgcgcgg ggcggacctg atcatcgcgg tcaataccga
    10261 tccgcgggca ccgatctacg gggttgccaa ctacggagcc gtggccgatg tcatcgacgt
    10321 cgtcccggcg ctgacgaagc agctccgcgg ctgaggcgcg gatgctggac gcgagcgtca
    10381 cgcgggaggt cttcgagggg ctgtcgcccg acggcgagct tgccttctac ctcctcgccg
    10441 ccctggccac gacgatcttc gtcgtcgggc tcggcctgcg gctgcggaag taccttcgcg
    10501 gccggcgtga ggatcgcgtc gggtccctga ccgcgttcat cggccgggcg atcggcggcg
    10561 ttcgcgcaac cgccacgagc gagacggtcc gcaagcgcga cgcgtacgcc gggatcttcc
    10621 acgccgcggt gatgtggggc ttcatcgtcc tcttcatcgg gacggtcatc ctcacgatcg
    10681 acaccgatat cgtcgggctc atcgcgccgc agtaccactt cttcaacggc gccttctacg
    10741 tcgtctactc gttcatcctc gacctcctcg ggctggccct gctcgtcggc ctggcagcgc
    10801 tggcctggcg gcgagcgcgg ttccacaagc gggagctgga ctacgcccgg gtcgacctcg
    10861 gggcggcgac cacggaccgt cgtccgttcg tgttcggcga ttggctgttc ctcggctggc
    10921 tcggcctgct cgggaccagc gggttcatcg tcgagggcgt ccggatcgtc gcccacgact
    10981 ttccatggtt cgaagcgttc tcgccggtcg gcgagatcct cgcccgggtc gttgcgttcc
    11041 tcggcctgag cgcggcgacg gccggctcgc tccacccctg gctgtggtgg ttccacgcga
    11101 tcgccgccct gggcttcgtc gcctacatcc cctacgccaa ggcgatccac atgctgaccg
    11161 acggggcgaa cctcgccctc cgatcgccgc tggccggccg ccgcctgccg gtcatcgatc
    11221 cggccccgcg acccctcctc gccggtctcg ccccggccgc cgccggcgac agccacatcg
    11281 gcatccgcga ccttgccgac ttcagctgga agcagctcct cgacttcgat gcctgcacca
    11341 agtgcggccg ctgccacgcc gcctgtccgg cggtcgcggc cggggcgccg ctgtcgccgc
    11401 gcgacctgat cctcgacctt cgccaacagg ccgacgcctc ctgggcggtc cgcggcccgg
    11461 gcatggagcg acgatccgac gtcacctacg gcggggcacg ggccgacgcc ggcgagccgg
    11521 tcttcagcga cgtcgcgacg cccgtgactc gcggtggact gatggccggt gggctggtca
    11581 aggccgagac gctgtgggcc tgcacgacct gcctcgcctg cgtcgaggcc tgcccggtcg
    11641 ggatcgagca cgtcccggca atcgtcggga tgcggcgctc gctcgtcgat cagggagccg
    11701 tcgagccggg gctccagacg gtcctcacca gtctcgccaa gcagggcaac tcgttcagcc
    11761 agtccggtcg cggccgggcg aagtgggccg acggcctgcc cacgccgctc cgcgacgccc
    11821 gcagggagga ggtcgactgg ctctggttcg ttggcgactt cgcctcgttc gacgttcggg
    11881 tccaggaggc gacccgtctc gtcgcgcgcg tcctccaggc cgccggagtc gatttcggga
    11941 tcctgttcga aggcgagcgg aacgccggca acgacgtccg ccgggtcggc gaggagggcc
    12001 tgttcgagat cctcgtcgaa cacaacgtcg gccagctcga gaaggcccgc ttccggcgga
    12061 tcgtgacgac cgatccccac accctgaaca cgctgcgcca cgagtacccg gacttcggcg
    12121 gccgctacga ggtctggcac tacagccagc tcctcgccgc cctgttcgat gacggcctgc
    12181 tccggacgcg acggccgatc gcccgtcgag cgacctatca cgacccgtgc tatctcgccc
    12241 gctacaacga cgtcacccga gcgccgcgcg acgtcctcgc cgcgatcggc gcacggctcg
    12301 tcgagatgcc gcgcaacggc acgaacacgt tctgctgcgg cgccggcggc gggcggatct
    12361 ggatggacga ctccgggctc cgcgagcgcc cgtccgagca gcggatgcgc gaggcggcgg
    12421 cgctcgagcg cgtcaccgac ttcgtgacgg cgtgcccgaa ggacctgacg atgtacacgg
    12481 ctgccgccaa ggcgaccggc ctcgacgacc ggctggtcgt gagcgacctg atcgaactcg
    12541 tcgcgcgggc gatcgacctc ggcgacgagg ttgccgagga gtcgtcgatg gacgacgagg
    12601 ttgcccttct tgcggcggcc gggtcggccg gtgcctgacg tgacggacgt gcctgacgtg
    12661 acggacgccg ccgtcggcgc ggtcgcggcc gacgccccgg tcgtggccga cgagctcctc
    12721 gaccggctgg ccgcggccgt tgcggctcgg gtccgcggcc gtcggacggc agaggagcgg
    12781 ctcggcgcgt tcgcgtttca cgaggggcca cgccacggcc gacaggcagg catggcaccg
    12841 ctcgatcggt cgacagccga cgagtcgacc ccggactcag ccgcgatcga ctggcgctac
    12901 ttcgtcctcc gcgccctcac tgccctcggt gatcccgacg ccgtccggat cctcgaggcc
    12961 ctgccacccg acggtcgacc cctcgacgcg ctggtcggtc tcgtcgagcc cgacgtcccc
    13021 gacccggtgg ccgccgccga tcggatcggt cgactcgccg cggcgggcct cgtcggtcgg
    13081 gacctcgaga gcgaccacgt gtcactcctg ccgctcgggg aggtgctgct cgagctggtc
    13141 cgcgacgtgg cgcggcgggc gagcgtcgcc gaccgatgat ctgcccgtac tgctccgcgc
    13201 ccgggacgag gatggagacg cacgcccacc ttggcgatgt ccatggtgac caggtccgga
    13261 ccttccgcga cgagcagcgc gacaagctca tgttcgccct cgactgcccg ttctgcgacg
    13321 agggcatgga gcgggtcgcc aatccccgcg gtcgtgaggc cggctttctc gatgagtttc
    13381 gccgcgagat cacgctcgtg gccttcgatc tcttgctgta tcacttccag gcatcccatg
    13441 ctgaacgcgt ccagctgccg ccggttccgg ccgacaccgg acccgagaca ggaggaaccg
    13501 gatgacgcaa gttcgcggct gcgaggtccc cgaggacctg tattactggg tggagaagca
    13561 cgtctgggcc cgccacgacg gcgatggact gctgacgatc ggggtcacgg acacggccca
    13621 gcacctcgct gcgcgggtcg tcgcgatcac cgccaagaag gtcggccgcc agatcgagcg
    13681 agggcagagc gtggccaccc tcgagagcgg caaatgggtc ggacctgtgc cggcgcccgt
    13741 cgacggcgag atcgccgagg tcaacccggc gatcgcgacc gatccgacga tcgtcaacag
    13801 cgacccgtac ggtgcgggct ggatcgtcaa gttccgggcc acggactgga gcggccagca
    13861 tgcccagctc gtcaccggcg ccgcgggtgt cgaggcctac cgcgccttcc tcgaagccga
    13921 gggcatcgcc tgtgattgag ggcctgcccg gacacgccgg ctcgtaccgc ggttgcgaga
    13981 tcccgcccga ccttctctat gacatcgagc gcgatgtctg ggtccggccg ggtcccgatc
    14041 ggacgctgac gatggggatg accgacccgg cccagtcgcg ggccggcaag ttccttcaca
    14101 tccagttcaa gaaggtcggc cgccatctcg aggccggtca gagcgcggcg acgatcgaga
    14161 ccgccaagtg ggcgggcccc ttcccgagtc cggttgccgg gacgatcgtc gacacgaacg
    14221 aggccgcgta cggggccgac atcctgatcg ccaaccgcga tccatacggg gccggttgga
    14281 tcgttcgcct cgcgccggac gacccgacgg ccgcgatctc caatctgatg accggcccgg
    14341 aggcggtggc cagctacgag gcgaggatcg atctccttgg gctgaactgc atccgctgcg
    14401 cggactgagg gaggaccgtc atgaacgcca ttcgcctgct cgacctcgga tcggtccccg
    14461 cgatccgctc gcaaaccatc tatcacgccg tcggctacgc cctcgggccc gagacgcccg
    14521 acacgatcat cctcgtgtcg cccgacgccc catacgtctc gatcggccgg caccaggatg
    14581 ccgaccgcga agtcgacctc gattactgtc gggaggccgg cctgccggtg atccgccgcg
    14641 aggtcggcgg cggcgccgtc tacctcgacg gcaaccaggt cttcacccag tgggccttcc
    14701 gggccgacca tctcccggcg accgtcgagg atcgcttccg gttgtacgtc gagacgctcg
    14761 ttcgggcgta tcgcacgctc gggatcgccg ccgaataccg gccgatcaac gacgtccacg
    14821 tcgcaggccg caagatcggc gggaccggcg cggcgcggat ggaccgggcc gaggtcgtcg
    14881 tcggcagcct gatgttcgac ttcgatatgg cgacgatggc ccgggtcctc aaggtcccgt
    14941 cggagaagtt ccgcgacaag gtccaccagt cgctgatcga ctacatgacg acgatgcgcc
    15001 gcgagctcgg cgagccgcca cctcgccagg tcgtcgttga cgcgtacgtg gccgcc
//
LOCUS       DATAIW010000173         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212317, whole genome shotgun sequence.
ACCESSION   DATAIW010000173 DATAIW010000000
VERSION     DATAIW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212317"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(541..1440)
                     /locus_tag="VHM48_05610"
     CDS             complement(541..1440)
                     /locus_tag="VHM48_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754917.1"
                     /translation="MPTRTVVLDGPLDLRRTLAIHQRGAGDPTMRLVAGRIVRATRTV
                     DGPATIALAGFADRVEAEAWGPGADRLLDAVPALIGLEDSRTGFEPHHHPLVAELDRR
                     QRGIRLGRTGAVLEALIPAILEQKVTGTEAWRGLRGLVRGWGEPAPGPFGLRLLPEPV
                     TIAAIPYHAFHPLGVERRRADLVRRVADHAGRFEEIPRLSRDAAYARLTALPGIGPWT
                     AAEVMLRTTGDPDAVSIGDFHLPNLVAFTLAGEIRGTDERMLELLEPWRGHRARVIRL
                     LETSGLRPPAFGPRHAPRSIAAI"
     gene            1679..2278
                     /locus_tag="VHM48_05615"
     CDS             1679..2278
                     /locus_tag="VHM48_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX2754918.1"
                     /translation="MRRLRVVAGMALIALLAAAAVPGSVGSQGPSPEQPIDAILFQGV
                     DAASIDGTAAMTPSLDPAYRSDGALSDTTVLLDPDERPAPAGRPDAAAIQPDAPVGSV
                     TIPQWHFDSNVSFYGPGFYGNRTACGYAMTKSLVGVAHRTLPCGTKVTFRNPANGRTV
                     TTIVVDRGPYVAGRDWDLTGGLCLALDHCYTGPLLWKLP"
     gene            2424..2690
                     /locus_tag="VHM48_05620"
     CDS             2424..2690
                     /locus_tag="VHM48_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX2754919.1"
                     /translation="MCRSIKVLRRPDEPATTGEIEAAARQFVRKVSGYRTPSQRNSAA
                     FEAAIEEIAAVSRKMLEEIGATVEVGPDHWHPGSGRARRPAAPA"
BASE COUNT          394 a          963 c          972 g          381 t
ORIGIN      
        1 accacccagc ggtcggcgaa caggcccgtg gccatgttgc cgccgacgaa cgccgccggg
       61 taaccgaaca tcttcttctg gaccgcatcc gggtacccgg ccatgaccgc cgcgaagcgc
      121 tcgacgagct ccggcgggga cttgctgaag cttggcatcg cgcgttcggc catcgccctg
      181 tctcctgcta ggtcggccta cggtagaccg atcgaccatg tcgcgggaat cccgcgtgga
      241 tcgcggcgcg ggtcgccgag ccgaagacgg cttcaagccg acccgaaggt cccgggcgga
      301 acctccgccg gacgactgtc ggtcgccgcg gcggccgcgc cggagtcccc ggagcgccgt
      361 cgggtcgccg ctgccgcgtc gttcgctccg accgggcgcg agcggcgctc gatcctggcc
      421 cagctcatgc acggcgcgac ggttcgacgc gcaacgacgt tacggctcgc gaccgccgag
      481 cggttccgga ggtcgagcgg tcgcgttctt ccgacggcgt cgggcgggcg accgggatcg
      541 tcagatcgcc gcgatcgacc gcggcgcatg ccgcggaccg aaggccggtg gccggaggcc
      601 gctcgtctcg aggaggcgga tcacgcgggc gcggtggcca cgccagggtt ccagcagctc
      661 cagcatccgc tcgtcggtcc cgcggatctc gccggccagg gtgaaggcca cgaggttcgg
      721 aagatggaag tcgccgatgc tcaccgcgtc ggggtcgccg gtcgttcgga gcatcacctc
      781 cgccgcagtc cacggcccga tgccgggcag cgcagtgaga cgtgcatagg cggcgtcgcg
      841 cgacagtcgc gggatctcct cgaaccgccc cgcgtggtcg gccacccggc ggacgagatc
      901 ggcccggcgg cgttcgaccc cgagcgggtg gaaggcgtgg tacgggatcg cggcgatggt
      961 cacgggctcc ggcaggagtc gcagcccgaa tggccccgga gcgggctcgc cccagccgcg
     1021 gaccagtccg cgcagcccgc gccaggcctc ggtgccggtg accttctgct cgaggatcgc
     1081 ggggatcagt gcctcgagaa cggcgcccgt ccgaccgaga cggatccccc gctggcgccg
     1141 atcgagctcg gcgacgagcg ggtggtgatg cggctcgaaa ccggtccgac tgtcctccaa
     1201 tccgatcagt gccgggactg cgtcgaggag ccggtcggcg cccgggcccc acgcctcggc
     1261 ctcgacgcga tccgcgaagc cggcgagcgc gatcgtggcc ggtccgtcga cggtccgggt
     1321 ggcgcggacg atccggccgg ccaccagtcg catcgtggga tcgccagcgc cacgctggtg
     1381 gatggcgagg gtccggcgga gatcgagggg tccgtcgagg accaccgttc tggtcggcat
     1441 gaccgtgaag tctgtcacgc gaggtcgccc ggcgggcagg caacggctga tgcgaggcgg
     1501 tcagaggcga ctgttgcggt agccgcaacg aggtgcgagg gcgcaccgat cgtgcgcgtt
     1561 tcgggacacc ggccgggggt cgccgtgcac gaatgctgcc tttgccacgc tcccgcaacg
     1621 catcgcaacc ctcccggcat gggcagaatc gagacaccct tggtaaggta ccgccgacgt
     1681 gcgacgcctc agagtcgtcg cgggcatggc gctcatcgcc ctgctcgccg cggccgccgt
     1741 ccccggatcc gtgggatccc agggccccag ccctgaacaa ccgatcgatg cgatcctctt
     1801 tcaaggggtg gacgcggcat cgatcgacgg gacagcggcg atgactcctt ctctcgatcc
     1861 tgcgtaccgg tctgacggtg cgctcagcga tacgacggtc cttctcgacc cggacgagcg
     1921 gcctgctcct gcaggccgtc ccgatgcggc cgcgatccag cccgacgcac ccgtcgggtc
     1981 ggtcacgatc ccgcagtggc acttcgactc gaatgtgtcg ttctacggtc ccggcttcta
     2041 cgggaaccgg accgcgtgcg gctacgcgat gacgaaatcc ctcgtcggcg tggcccaccg
     2101 gaccctgccc tgtggaacga aggtcacctt ccgcaatccg gccaacggcc gtacggtcac
     2161 gacgatcgtc gtcgaccgcg gtccgtacgt ggcggggcgc gactgggacc tgacgggcgg
     2221 gctctgcctg gccctggacc attgctacac cgggccactc ctctggaagc tcccgtagct
     2281 cgctgacgac gaccggaccg accggccgtg cagacacgct gcccaacgca gcgaagctcc
     2341 cttccgttcc ggcggggagc ttcgtcgcgc ccgctggcgg tcaatcgcgg agccagatgg
     2401 tcgaacgcgg taccctgccc ggcatgtgcc gaagcatcaa ggtccttcgc cgtccggacg
     2461 agccggcgac gaccggtgag atcgaggcgg ccgcccgcca gttcgtccgc aaggtcagcg
     2521 gctaccgcac gccgtcccag cgcaacagcg ccgccttcga ggcggcgatc gaggagatcg
     2581 cggccgtgtc gcggaagatg ctcgaggaga tcggggccac ggtcgaggtc ggaccggatc
     2641 actggcatcc gggttcgggt cgcgcgcgcc ggccggccgc cccggcgtga ccgtcgaggc
     2701 gtcgcgagga
//
LOCUS       DATAIW010000174         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_158020, whole genome shotgun sequence.
ACCESSION   DATAIW010000174 DATAIW010000000
VERSION     DATAIW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158020"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..>2515
                     /gene="topA"
                     /locus_tag="VHM48_05625"
     CDS             194..>2515
                     /gene="topA"
                     /locus_tag="VHM48_05625"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX2754920.1"
                     /translation="MPKNLVIVESPAKARTIERYLGADYRVLASYGHVRDLPENPGKG
                     KFGVDVEHDFEPEYVISDDRRKQVAEIEKAARSADAIFLATDLDREGEAIAWHVAEAA
                     HVAAAKTFRVTFNEITESAIREAFANPRQIDMDLVDAQQARRIVDRLVGYTLSPLLSR
                     KVRGGLSAGRVQSVAVRLVVEREREITAFRAREYWTIEALLATVAGERFSADVVKIDG
                     APLDVVDEATAERHRAAIAQLHPSVTKLGTRTQKRSPAPPFTTSTLQQEASRRLSFSP
                     KRTMSIAQRLYEGIATGEGQVGLITYMRTDSTAIAGVAMGEAREVIGERYGDRYTMPK
                     GRVYKTKSKGAQEAHESIRPTSFRRDPDSVASSLKPEELRLYRLIWQRAIASQMSPKE
                     LETTTIDLAEDGYELRASATKVLFDGFARVYTEGRDDDAADDDEETGRLPTLVAGDTT
                     SVEEVTPTQHFTEPPPRFTEATLIKALEEHGIGRPSTYAATISTITDRGYVRVAERRL
                     HPEPVAEIVTDLLVEHFGDYVDVTFTARMEEELDEVARGEREWVPLLRAFYGPLRDRV
                     EEKRRELKRSDFTTEPTDEVCSLGHPMVIRLGRNGRFLACSLYPEHKESRPLPGDEPP
                     PQEGTGEVCPKCNEGTLVGKRGRFGPFVGCSRYPDCDYIKKDGPPPPDPLPFEVTCPK
                     NKDGHLVPRRARRTGNVFWGCSAYPRCDFTTNNEPLGGLHDVDAGPLARKDESAICLV
                     CGSTSEVAAAAVVPGETYAGGPANPEALARPARA"
BASE COUNT          435 a          871 c          856 g          353 t
ORIGIN      
        1 gatgtcgggc agcgatccgt cactggccgt gacgatggac gacgaggccg ggcgatccag
       61 tcgaccgccg gcggcggagc ctgaacccga tctcgagatc gacgaggatt tcctgcgccg
      121 catccgcgag gtctgagccg ggtccgggat ctggtgaagg gtccgataag gacccagtgc
      181 tacactcccg gccgtgccaa agaacctggt catcgtcgaa tcgcctgcca aggcccgcac
      241 catcgagcgc tatctcggcg ccgactaccg ggtcctggcg tcctatggcc acgtccgcga
      301 cctgccggag aatcccggca agggcaagtt cggggtggac gtcgagcacg acttcgagcc
      361 ggaatacgtc atctcggacg accgtcgcaa gcaggtcgcc gagatcgaga aggccgcccg
      421 cagcgccgac gccatcttcc tcgcgacgga cctcgatcgc gagggcgagg cgatcgcctg
      481 gcatgtcgcg gaggcggccc acgtcgccgc cgccaagacg ttccgggtca ccttcaacga
      541 gatcacggag tccgccatcc gcgaggcgtt cgccaatcct cgccagatcg acatggacct
      601 cgtggacgcc cagcaggccc gtcgcatcgt cgatcgactc gtcggctaca cgctcagccc
      661 gttgttgtcg cgcaaggtcc gcggtgggct gtcggccggg cgtgtccagt cggtcgcggt
      721 gcgcctcgtg gtcgagcggg agcgcgagat caccgccttc cgggcgcgcg aatactggac
      781 catcgaggcg ctcctcgcga cggtcgccgg cgagcgtttc agcgccgacg tggtcaagat
      841 cgacggcgcg ccgctcgatg tcgtcgacga ggcgaccgcc gagcgccacc gcgccgcgat
      901 cgcccagctc catccgagcg tgaccaagct cgggacgcgg acccagaaac ggtcaccagc
      961 accgccattc acgacctcga cgctccagca ggaggcaagc cgccggctgt cgttcagtcc
     1021 gaaacggacg atgtcgatcg cccagcgcct gtacgagggg atcgcaacgg gcgagggaca
     1081 ggtcggtctc atcacctaca tgcgaacgga ctcgaccgcg atcgccggcg tggccatggg
     1141 cgaggcgcgc gaggtcatcg gggagcgcta cggcgaccgg tacacgatgc ccaagggccg
     1201 ggtctacaag accaagtcga agggcgccca ggaagcccac gagtcgatcc gcccgacgag
     1261 cttccggcgc gatccggact ccgtcgcgtc ctcgctcaag ccggaggagc tccggctcta
     1321 ccgcctgatc tggcagcgcg ccatcgcctc gcagatgtcg cccaaggaac tcgagacgac
     1381 cacgatcgac ctggcggagg acggctacga actgcgggca tccgcgacga aggtgctgtt
     1441 cgacgggttc gcccgggtct ataccgaggg ccgcgacgac gatgccgccg atgacgacga
     1501 ggagaccggg cgattgccca cgctcgtggc cggcgacacc acctccgtcg aggaggtcac
     1561 accgacccag catttcacgg agccgccgcc gcgcttcacg gaggccaccc tgatcaaggc
     1621 cctcgaggag cacgggatcg gtcgtccgtc cacgtatgcc gcgacgatct cgacgatcac
     1681 cgaccggggc tacgtccggg tcgcggaacg acgactccac cctgaaccgg tcgccgagat
     1741 cgtgaccgac ctgttggtcg aacacttcgg ggactacgtg gacgtcacgt tcacggcccg
     1801 gatggaagag gaactcgacg aggttgcccg cggcgagcgc gaatgggtcc cgctcctgcg
     1861 ggcgttctac ggtccgctgc gcgaccgggt cgaggagaag aggcgcgaac tcaagcgcag
     1921 tgacttcacg acggagccga ccgacgaggt ctgctcgctg ggtcacccga tggtcatccg
     1981 gctcggtcgg aacggtcggt tcctggcctg ctcgctctac ccggagcaca aggagagccg
     2041 cccgttgccc ggggatgaac cgccgccgca ggaggggacc ggcgaggtct gtccgaaatg
     2101 caacgagggc acgctcgtgg gcaagcgcgg ccggttcgga ccgttcgtcg gttgctcgcg
     2161 ctatcccgac tgcgactaca tcaagaagga cggaccgccg ccgccggatc cgctcccctt
     2221 cgaggtcacc tgcccgaaga acaaggacgg gcatctggtc ccgcgtcgcg cgcggcggac
     2281 cgggaacgtc ttctgggggt gttccgccta tccccggtgc gatttcacga cgaacaacga
     2341 accgctcggt gggctccacg acgtggacgc cggtccactg gcacgcaagg acgagtcggc
     2401 gatctgcctg gtctgtggct cgacgagcga ggtcgcggcc gccgccgtcg tcccaggcga
     2461 gacctacgcg ggcggcccgg cgaacccgga ggccctggcg cgtccggccc gcgcc
//
LOCUS       DATAIW010000175         6413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_182760, whole genome shotgun sequence.
ACCESSION   DATAIW010000175 DATAIW010000000
VERSION     DATAIW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182760"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VHM48_05630"
     CDS             complement(<1..986)
                     /locus_tag="VHM48_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2754921.1"
                     /translation="MADPSPGLPRPRFRSRLAARPLLVDGGMGTLLFSRGVPQRACLE
                     ELVLNQPEMVGAAHREYLEAGAELIETLSFGANRHRLAAWGLEGSVGDLNRRAARIAR
                     EAREVSGREALVGGSVGPLGSPARGMPSMSDATMRAIFREQIEALLEGGSDLIVLETF
                     SDINQLVLAVDEARLASDGPVLASLTFGEELRLTDGSSPTAAARALAAAGADAIGVNC
                     GAGPTACLDALVAMGDPADGEPARSIMPNAGLSQRLEGRFVFAASPEYFGAVTPRMLA
                     AGARILGGCCGTTPEHIAAMRLALDAVETSGASVGQASALADAPDVSTASNA"
     regulatory      complement(1135..1241)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1313..1801)
                     /locus_tag="VHM48_05635"
     CDS             complement(1313..1801)
                     /locus_tag="VHM48_05635"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2754922.1"
                     /translation="MASGNRLQPRLVLLCGLPASGKTTLARELADAYGAVLLNPDEWE
                     LALDVDPFDEGFQARLEAEFWKLTQRLLVPGTSVVLEWGFWARSERDEKREAARTLGV
                     AVELRFLDAPYDELVRRVVARHAAGGIAITESHMERYRGTFQPPTDDELALYDPPLLE
                     AN"
     gene            complement(1915..2793)
                     /locus_tag="VHM48_05640"
     CDS             complement(1915..2793)
                     /locus_tag="VHM48_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754923.1"
                     /translation="MLFRRRASLAPPAREALARVLRAPTFELVPLKNALDQAEFLPAG
                     VTVSVTASPAKGIEATIALCEQLQKRGFRAVPHLAARMVRDRSHLADLIAWLEGAGVD
                     RAFVVGGDAKEPGDYPDGLSLLRDMAEIGHPLSEIGIPCYPQGHAFIADGPLLEALHA
                     KATFASYMTTQLCFDPGAIATWLAARRAEGLELPVHVGVPGVAEPHKLLAISARIGVA
                     DTHRFLTKNLRFVARLVRSGGFYRPDALLEGLAPHITDPAARIVDLHMYTFNAVDTFE
                     AWRGSYLAKLVRAVPT"
     gene            complement(2805..4349)
                     /locus_tag="VHM48_05645"
     CDS             complement(2805..4349)
                     /locus_tag="VHM48_05645"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2754924.1"
                     /translation="MTVEESPRQRTGGRAGRQAARASHHIAREPFLTRTLAPFEVLND
                     EGLAIIEENADTILQEVGLEFRGDPDALRLLRAAGADVQGERVRFPRGMCRQIVQATA
                     PRQFTQFARNTANNVEIGGRHTVFAPNYGSPFVRDLDNGRRYGTLEDFRNFVKLAYMS
                     PFLHHSGGTVCEPVDVPVNKRHLDMVYAHMRWSDKPFMGSVTAPERARDTVEMARILF
                     GAEYLEDHAVVLSLINANSPLVWDSTMLGAARAYAEANQATLITPFILAGAMSPVTVA
                     GAAAQTLAEALGGMTFVQLVRPGAPVVLGSFASSMSMQSGAPTFGTPEPALVLYVMAS
                     LARRLGVPFRSGGNLCASKIADAQAAYESAATFQPTILAGVNFVLHAAGWLEGGLAVG
                     YEKFILDADQCGMAAVFVKGVDLSENGQALDAILTNGPGQHYLGAAHTLANFENAFYR
                     SEIADNNSFEQWQEDGSLDAAQRANAIWKRMLREYEAPPLDAGIDEALGEFIAKRKAS
                     MPDSEV"
     gene            complement(4346..>6413)
                     /locus_tag="VHM48_05650"
     CDS             complement(4346..>6413)
                     /locus_tag="VHM48_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX2754925.1"
                     /translation="ASVASDMSGEPLVIFTPSGRRGQFAPGTTVLDAARRLGVDIDSV
                     CGGRGICGRCQVEQSVGEFAKHGITSAPDHLTPFGAVEAEYRDRKGLAHERRLGCMAH
                     VRGDVVIDIPPESQVFRQVVRKGLDVRDFHLDPVVRLHYVEVEPPQLASPSGDLHRLQ
                     LALESEWGLHDLEPDLHVIRALQPALEAGKYGVTVAVHDGRTISAVWPGLHEQAYGVA
                     IDIGSTTIAGHLANLGDGAVLASAGVMNPQIRFGEDLMSRVSYAMMNEAGAGEMTVAV
                     RQALNGLLAQLANRAGIKRDAILELTIVGNPIMHHLVLGIDPIPLGSAPFALATDRAL
                     TIRADELDLLTHPGARVYVLPCIAGHVGADTAGVILAEAPHESEVIELVVDVGTNAEI
                     VLGNKDFLLAASSPTGPAFEGAQISSGQRAAPGAIERVRIDRDTLEPRVRVIGSELWS
                     DEPGFAAATADSGITGICGSGIVEVIAELFLAGVITHDGIVDGSLAARNPRIVAEGRT
                     FSYLVHDGSGEGRLRITVTQNDVRAIQLAKAALYAGVRLLMDHAGIDAVDEIRLAGAF
                     GSQIDPFHAMVLGLIPDAPLDHVRAAGNAAGTGALIALLSAAARREIEDVVRRVEKIE
                     TAVEPRFQEHFIEAMAFPHRTAPSTHLEQVTTLPQRGAGPAGGARPDGGGRRRRAVAA
                     AAKEER"
BASE COUNT          972 a         2162 c         2322 g          957 t
ORIGIN      
        1 ctggcattgg acgcggtcga gacgtccgga gcatcggcga gcgcggaggc ctggcccacc
       61 gatgctccgg acgtctcgac cgcgtccaat gccagtcgca tcgcggcgat gtgctcgggc
      121 gtcgtgccgc agcagccgcc gaggatccgg gcaccggccg ccagcatccg cggcgtcacg
      181 gcgccgaagt actcggggct ggcggcgaat acgaagcgtc cctcgagccg ctgcgacagt
      241 ccagcgttcg gcatgatcga acgagctggc tccccgtcgg ccggatcgcc cattgcgacg
      301 agtgcgtcga ggcaggccgt cggcccggca ccgcaattga ccccgatggc gtccgcgcca
      361 gccgcggcga gggctctggc tgcggccgtc gggctgctgc catcggtaag gcggagttcc
      421 tcaccgaacg tcagcgaggc gaggaccggc ccgtccgacg ccagacgcgc ctcatcgacc
      481 gcgaggacga gttggttgat gtccgagaag gtctcgagga cgatcaggtc gctaccgccc
      541 tcgagcagcg cttcgatctg ctcacggaag atggccctca tcgttgcgtc ggacatggac
      601 ggcatgccgc gcgccggcga cccgagcggc ccgacggatc cgccgaccag cgcctcgcgc
      661 ccgctcacct cccgcgcctc gcgggcaatg cgcgcagccc gacggttgag gtcaccgacg
      721 ctgccctcga ggccccaggc cgcgaggcga tgacggttgg cgccgaacga cagggtttcg
      781 atgagctccg cacccgcctc cagatattct cgatgggctg cgccgaccat ctccggctga
      841 ttcaagacca gctcctcgag acaggctcgc tggggcacgc cgcgactgaa cagaagcgtg
      901 cccatgccgc cgtcgaccag gagcggccga gccgccagac ggctgcggaa gcgcggccgc
      961 ggcaggccgg gcgaggggtc ggccatcagg cgttcggagt ccgaatgggt tcggtcggca
     1021 agatctcggc tcctcgattc gttcggcgtg ctggtgacgc cggtggatgg atcgggcgat
     1081 cgagaccgga aacggaaaga cccttcccgc tcggaagggt cctgggggag aggtccctta
     1141 tcgtgcagga gaacctgccc ggaattggca ccttgccggc gcgacgccgg taggttgtcg
     1201 tggcttcaac gggccggtcc ctccaccact ctggataagc gctcttcagt tgtgaccgca
     1261 tggtgcccga gcccgcccga ttgagcaagc gggcggtcag gtcggtagtg tctcagtttg
     1321 cttcgagtag aggggggtca tagagggcca gctcatcgtc agtcggcggc tggaaggtgc
     1381 ctcgataccg ctccatgtga ctctcggtga tggcgatgcc gccggcggcg tgacgagcca
     1441 cgacccgcct caccaactca tcgtagggcg catcgaggaa acggagttcg accgctacgc
     1501 cgagcgttcg tgcggcctcg cgcttctcgt cgcgctccga gcgagcccag aagccccatt
     1561 cgaggacaac cgaggttccc ggcacgagga gccgctgcgt aagtttccag aattccgctt
     1621 cgagcctggc ttggaagccc tcgtcgaacg gatcaacgtc caacgccaac tcccactcat
     1681 ccgggttgag cagcaccgcg ccataggcgt ccgcaagctc gcgcgcgagc gtcgtcttgc
     1741 ccgatgcagg caagccgcac agcaacacaa gcctcggttg cagcctgttg cccgacgcca
     1801 tcctggcgct cctccttcgc ggccattgtc gccggacggc ggcgaagggt gaagatttcg
     1861 gaccagcgat caggagtcag ccgagtagat ccgcgatcta ttcagccaca accgtcaggt
     1921 cggaacggcc ctgaccagct tcgcgaggta cgagccccgc caggcctcga acgtgtcgac
     1981 ggcgttgaac gtgtacatgt ggaggtcgac gatccgggcc gcgggatcgg tgatgtgggg
     2041 cgccagcccc tcgagcaacg cgtccgggcg gtagaagcca ccggagcgga cgaggcgggc
     2101 gacgaatcga aggttcttcg tcaggaaccg gtgggtgtcg gccacgccga tccgggcgct
     2161 gatcgcgagg agcttgtgcg gctcggcgac cccgggcacg ccgacgtgga ccggcagctc
     2221 gagcccctcg gcccgacgcg ccgcgagcca ggtcgcgatc gcgcccgggt cgaagcagag
     2281 ctgagtcgtc atgtagctcg cgaacgtggc cttggcgtgc agcgcctcca gcaacggccc
     2341 gtcggcgatg aacgcgtggc cctgcgggta gcacgggatc ccgatctccg aaagcggatg
     2401 cccgatctcg gccatgtcgc gcagcagcga caggccgtcc gggtagtcgc caggctcctt
     2461 ggcgtcgccg ccgacgacga acgcccggtc gacgccggcg ccctcgagcc aggcgatgag
     2521 atcggcgagg tggctgcgat cgcggaccat ccgggccgcg agatgcggca cggcgcggaa
     2581 tccgcgcttc tgcaactgct cgcagagagc gatcgttgcc tcgatcccct tggctggcga
     2641 ggccgtgacc gaaaccgtga ctccggcagg caggaactcg gcctggtcga gggcgttctt
     2701 cagcggcacg agctcgaacg tcggggcacg gaggacgcgc gccagggctt cacgcgcggg
     2761 cggtgccagt gatgctcgac gcctgaacag catgcgtgcc gaccctagac ttcggaatcc
     2821 ggcatcgacg ccttgcgctt cgcgatgaac tcgccgagcg cctcgtcgat ccccgcgtcg
     2881 agcggcggcg cctcgtactc gcggagcatc cgcttccaga tcgcgttggc ccgctgggcg
     2941 gcgtcgagcg agccgtcctc ctgccattgc tcgaagctgt tgttgtcggc gatctcgctg
     3001 cggtagaagg cgttctcgaa attggccagg gtatgggcgg cgccgagata gtgctggccg
     3061 ggtccgttcg tcaggatcgc gtcgagcgcc tggccgttct ccgagaggtc gacacccttg
     3121 acgaacaccg cggccatccc gcactggtcg gcatcgagga tgaacttctc gtagccgacc
     3181 gcgagcccgc cctcgagcca gccggcggcg tggagcacga agttgacgcc ggcaaggatc
     3241 gtcggctgga aggtggcggc cgattcgtag gcagcctggg catcggcgat cttgctcgcg
     3301 cagaggttgc cgccggagcg gaacggaacg ccgagccgcc gggcgagcga ggccatgacg
     3361 tacagcacga gggccggctc cggggtcccg aacgtcggcg cccccgactg catcgacatc
     3421 gacgacgcga agctgccgag gacgaccggc gcacccggcc ggacgagctg gacgaaggtc
     3481 atcccgccga gtgcctcggc gagcgtctgg gcagcggccc cggcgacggt caccggcgac
     3541 atcgcaccgg ccaggatgaa cggcgtgatc agggtcgcct ggtttgcctc ggcataggcc
     3601 cgggcggcgc cgagcatcgt cgaatcccag acgagcgggc tgttggcatt gatcagcgac
     3661 aggacgacgg cgtggtcctc gaggtactcg gcgccgaaca ggatccgggc catctcgacg
     3721 gtgtcgcggg cccgctccgg cgcggtgacg gagcccatga acggcttgtc gctccagcgc
     3781 atgtgggcgt agaccatgtc gaggtggcgc ttgttgaccg gcacgtcgac cggctcgcag
     3841 accgtccctc cgctgtggtg gaggaacggg ctcatgtacg cgagcttcac gaagttgcgg
     3901 aagtcctcga gcgtgccgta gcgccggccg ttgtcgaggt cgcggacgaa cggcgaaccg
     3961 tagttcgggg cgaagaccgt gtgccgcccg ccgatctcga cgttgttggc ggtgttccgg
     4021 gcgaactggg tgaactggcg gggcgcggtc gcctggacga tctggcggca catcccgcgt
     4081 gggaagcgga cccgctcgcc ctggacgtcg gcgccggccg cgcggaggag gcggagggca
     4141 tcggggtcgc cacggaactc gaggccgacc tcctggagaa tcgtgtccgc gttctcttcg
     4201 atgatcgcga ggccctcgtc gttgaggacc tcgaacggcg ccagggtgcg ggtcaggaac
     4261 ggctcgcggg cgatgtgatg gctggcccgg gcagcctgcc ggccggctcg accgccggtc
     4321 cgctggcggg gagattcctc gacagtcatc gctcctcctt ggcggctgct gcgaccgccc
     4381 gtcgacgccg tccgccgccg tcgggccggg ccccaccggc cggtccggcg ccgcgctgcg
     4441 gcagtgtcgt gacctgctcg agatgggtcg acggcgccgt ccgatggggg aaggccatcg
     4501 cttcgatgaa gtgctcctgg aaccgcggct cgaccgcggt ctcgatcttc tcgacgcgtc
     4561 gcacgacatc ctcgatctcg cgccgcgcgg ccgcgctgag aagcgcgatc agcgccccgg
     4621 tgcccgcggc attgccggcc gccctgacat ggtcgagcgg ggcatccggg atcagcccga
     4681 ggaccatcgc gtggaacgga tcgatctggc tgccgaacgc gccggccagg cggatctcgt
     4741 ccacggcgtc gatcccggcg tggtccatga ggaggcgcac gccggcgtac agggcggcct
     4801 tggcgagctg gatcgcccgg acgtcgttct gggtcaccgt gatccgcagc cggccctcgc
     4861 cggagccgtc gtggacgagg tagctgaacg tccggccctc ggcaacgatc cgcgggttcc
     4921 gggcggcgag cgagccgtcg acgatcccgt cgtgggtgat gaccccggcg aggaacagct
     4981 cggcgatgac ctcgacgatg ccggagccgc agatcccggt gatcccgctg tcggcggtcg
     5041 ccgcggcgaa ccccggctcg tccgaccaca gctccgagcc gatcacccgg actcgcggct
     5101 cgagcgtgtc gcggtcgatc ctgacgcgct cgatcgcccc tggcgcggcg cgctggccgg
     5161 acgagatctg ggcgccctcg aacgcggggc cggtcggcga ggaggcggcg aggaggaagt
     5221 ccttgttgcc gagcacgatc tcggcgttcg tgccgacgtc gacgacgagc tcgatgacct
     5281 ctgactcgtg gggcgcctcg gcgaggatca ccccggccgt gtccgcgccg acgtgaccgg
     5341 cgatgcacgg caggacatag acgcgggcgc cggggtgcgt caggaggtcc agctcgtcgg
     5401 cgcggatcgt cagcgccctg tccgtggcca gggcgaacgg cgccgagccg agcgggatcg
     5461 ggtcgatccc gagcacgagg tgatgcatga tcgggttgcc gacgatcgtg agctcgagga
     5521 tcgcgtcgcg cttgatcccg gcccggttcg cgagctgggc gagcaggccg ttgagcgcct
     5581 ggcggaccgc gaccgtcatc tcgccggcgc cggcctcgtt catcatcgcg tagctgaccc
     5641 ggctcatcag gtcctcgccg aagcggatct gggggttcat caccccggcg ctggcgagga
     5701 ccgcgccgtc gccgaggttg gcgagatgac cggcgatcgt cgtcgagccg atgtcgatcg
     5761 cgaccccgta ggcctgctcg tgcagtccgg gccagaccgc gctgatcgtc cgcccgtcgt
     5821 ggaccgcgac cgtgacgccg tacttgcccg cttccagcgc cggctggagg gcacgaatca
     5881 cgtggagatc cggctcgaga tcgtggaggc cccattccga ctcgagggcg agctggagcc
     5941 ggtgaagatc gcccgacggc gacgcgagct ggggcggctc gacctcgacg taatggagcc
     6001 ggacgaccgg gtcgaggtgg aagtcgcgga cgtcgaggcc cttccggacg acctggcgga
     6061 agacctgact ctcgggtggg atgtcgatca cgacgtcgcc gcggacgtgg gccatgcagc
     6121 cgaggcggcg ctcgtgggcg aggcccttcc ggtcgcggta ctcggcctcg acggctccga
     6181 acggggtcag gtggtcgggc gcggaggtga tcccgtgttt ggcgaactcc ccgacgctct
     6241 gctcgacctg gcagcgcccg cagatcccgc gcccgccgca gaccgagtcg atgtcgacgc
     6301 cgaggcggcg ggccgcgtcg agaacggtcg tccccggcgc gaactggccg cgccggccgg
     6361 acggcgtgaa gatgacgagg ggttcgccgg acatgtcgct cgctacgctc gca
//
LOCUS       DATAIW010000176         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_182807, whole genome shotgun sequence.
ACCESSION   DATAIW010000176 DATAIW010000000
VERSION     DATAIW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182807"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1445)
                     /locus_tag="VHM48_05655"
     CDS             complement(261..1445)
                     /locus_tag="VHM48_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754926.1"
                     /translation="MRVRTLELRLIAFALAMCWVVAAGLVLVAYRPGGPIDVAVGVTA
                     ILPAIIAFAGVRWPPVARGQRAFTVMVWLAAGSLLVLVPSIADVTGQLGGRGVQTLLP
                     SVEAGYPWVLALIGTCLFSGFGIARRRLGEAAMRRRRLVRGLGVATLLAAGTGMVFAA
                     VAFGNELALRDRVAASSRFGPTNFDRDPPPCDGPMGIGASAQISVEIEGVLDGRSMGT
                     IELGGERDRSDVRWLAYVATSRELGLHGAASIGADAWLREPFAVWRRSTAAEIGDVDL
                     DLTAFRVALSPEARAAAGTQGVGVIEGARARQCRILIDGPTFRAAFPQIVWLVGDADL
                     GHWRGHLDYWVFLDGQIGRIAGSVNGEATTIQPGALQATIRVDLAATDRGAPVSVTAP
                     TP"
     gene            1854..2051
                     /locus_tag="VHM48_05660"
     CDS             1854..2051
                     /locus_tag="VHM48_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754927.1"
                     /translation="MPTRSTYLNLIMRGLAPDEAANLTAYLAGIGVGGSHWTLRQINQ
                     LLFLREMNRTGQFAEGRDRIH"
     gene            2233..3048
                     /locus_tag="VHM48_05665"
     CDS             2233..3048
                     /locus_tag="VHM48_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX2754928.1"
                     /translation="MTGPLISFLTDFGLRDPSAAIMRGVVLSIAPDARIVDISHEVCK
                     FAIRDGALLLWCALPYLPVGFHVAVVDPGVGTARLPVAIATVRGDVLIGPDNGLLVAA
                     AGRLGGIRAVHVLESAAHRLPVVSNSFHGRDIFAPAAAHLACGVAITELGRELDPAAL
                     VPSPIREPEIRDGELGSSVVYVDTFGNVKLAGLQADLEAALGRIKAGDALELTIGGRR
                     VDTGWQTTFGEVAPGETLVYEDSYGRICLAASQANAAAIHGIVDDLEVVIRRR"
     gene            3119..>3314
                     /locus_tag="VHM48_05670"
     CDS             3119..>3314
                     /locus_tag="VHM48_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754929.1"
                     /translation="MRRSIPVTALLVSLALGACTSGATPSPSSSAAPSVAASTAPSVA
                     PSVAPSATPDACAKDNLKLTT"
BASE COUNT          502 a         1260 c         1105 g          447 t
ORIGIN      
        1 gtgggggaac cgacgatcga ctccccgaca tgggcgtatg cggctattcg aaacggcccg
       61 gcggccgcgc gcgccgacgg ctagccggga tccgcgagcg ccgcacgcga gcacattccc
      121 cgcagcgttt cgaatagccg actgtttcga ggctcatggg cggatcgggg ttggcgccga
      181 cgccggggta tgcggctatt cgaaacggta tggggcccct cgcgcgccag cggcgttcgc
      241 gcctcgcgcg actcgcgggc tcacggtgtc ggcgccgtga cggagaccgg cgcgcctcga
      301 tcggtcgccg ccaggtccac ccggatcgtc gcttgcaggg cgccgggctg gatcgtggtc
      361 gcctcgccgt tgacgctgcc ggcgatccgg ccgatctggc cgtcgaggaa gacccagtag
      421 tcgaggtggc cgcgccagtg gccgagatcg gcgtcgccga ccagccacac gatctggggg
      481 aacgcggccc ggaatgtcgg cccgtcgatg aggatccggc actgccgggc acgcgccccc
      541 tcgatcacgc ccacgccctg cgtgcctgcc gccgcccggg cctcgggcga cagcgcgacc
      601 cggaatgcgg tcaggtcgag gtccacatcg ccgatctcgg ccgcggtcga gcgccgccag
      661 accgcgaacg gctcccgcag ccaggcgtct gccccgatcg acgccgcacc atggagaccg
      721 agctcgcgac tcgtcgccac gtacgccagc cagcgcacgt ccgatcgatc ccgttcacca
      781 ccgagctcga tcgtgcccat cgatcggccg tcgaggacgc cctcgatctc gacggaaatc
      841 tgggccgacg cgccgatccc catcggaccg tcacaggggg gcggatcccg gtcgaagttc
      901 gtcggcccga accgcgacga cgccgctacc cggtcgcgaa gcgcgagctc gttgccgaag
      961 gcgaccgcgg cgaagaccat cccggtgccg gccgccagga gcgtcgcgac gcccagtccg
     1021 cgaaccagcc ggcgccgccg catggcggcc tccccgagcc ggcgccgggc aatcccgaag
     1081 ccgctgaaca ggcacgttcc gatcagggcg agaacccacg gatagccggc ctcgaccgac
     1141 ggcagcaacg tctggacccc acgtccgccg agctgtccgg tgacatcggc gatcgacgga
     1201 acgagcacca gcaacgatcc cgcggccagc cagaccatca ccgtgaacgc ccgttggccg
     1261 cgcgcgaccg gcggccagcg gaccccggcg aaggcaatga tcgccggcag gatcgccgtc
     1321 acgccgacgg ccacatcgat cggaccgccc ggccggtagg cgaccaggac gagaccggcc
     1381 gcgacgaccc agcacatcgc cagggcgaaa gcgatcaggc gcagctcgag ggtccggaca
     1441 cgcacggcgg gcagtctact cggccgccgt ctgtcgatcg ccgcagtgcg tcgccgcagg
     1501 gcgtcgccgc agggcgtcgc cgcaagtacc gggcgacatg ggaccgacgg gtcatcgttc
     1561 cgacgggcga accgaccgaa catcgcaact aaccacaccg gagcgacccg atcgccggat
     1621 ccgatggtcc gtcaccgggg cccggaacgt acccctcaat gggaccaaca gaagacggtc
     1681 agaaggacag gacggaacgg tgcaccatgg caggtcgacg cggaacccag ggtcgggctc
     1741 ggtcatcgcg cgccgactcg tcgtggccgg ttctcgtggc ggcctccgcg gccgcgtctg
     1801 ggtcgggcat cgccaccacg acctcggtcc tcaacccgag cccgagaccg gaattgccca
     1861 cccggtcgac ctacctcaac ctgatcatgc ggggcctggc gcctgacgag gcggccaacc
     1921 tcaccgccta cctcgccggc atcggcgtcg gcggatcgca ttggacgctc aggcagatca
     1981 accagctcct cttcctccgc gagatgaacc gcaccggcca gttcgccgaa gggcgcgacc
     2041 ggatccactg acgcatcggg cccgatggcc cacccgaacc gaatccaccc gtcgcggtga
     2101 cgtacctcct cccgtcaccg cggcactgct ccccgaagcg gcgccttccc tcctccaggc
     2161 gccgcttcgg ggtttcctgc gctcccttgc gggtctcgcc tccgatcgga ggtctgacgt
     2221 aggatcgcct gcgtgaccgg tccgctgatc tcgttcctga ccgatttcgg gctgcgcgac
     2281 ccgagcgccg cgatcatgcg cggcgtcgtc ctctcgatcg cccccgacgc ccggatcgtc
     2341 gacatcagcc acgaagtctg caagttcgcg atccgcgacg gggcgctcct cctgtggtgc
     2401 gccctgccgt acctgccggt cgggttccat gttgcggtgg tcgatcccgg ggtcggcacg
     2461 gcccgcctgc cggtggcgat cgcgacggtc cgcggcgacg tcctgatcgg cccggacaac
     2521 gggctcctcg tcgcggcggc agggcgcctc ggcgggatcc gggcggtcca cgtcctggaa
     2581 tccgcagcgc atcgtctgcc cgtcgtcagc aacagctttc acggtcgcga catcttcgcc
     2641 ccggccgccg cccacctggc ctgtggcgtt gcgatcacgg agctcggacg ggagctcgac
     2701 ccggctgccc tcgtgccgtc gcccatccgt gagcccgaga tccgcgacgg cgagctcggt
     2761 tccagcgtcg tgtacgtcga caccttcggc aacgtgaagc tggccggtct gcaggcagac
     2821 ctcgaggcgg ccctcggccg gatcaaggcc ggcgacgcgc tcgaactcac gatcggcggc
     2881 cgccgggtcg acacgggctg gcagacgacg ttcggcgagg tcgcgccggg cgagacgctc
     2941 gtctacgagg attcgtacgg ccggatctgc ctggccgcga gccaggcgaa cgccgcggcc
     3001 atccatggca tcgttgacga cctcgaggtg gtgatccgcc gccgctgatc cggtcggagc
     3061 ccgaccgccg atccgctaca ctccgcgggc gttcgctccg ttcacgaggt caccgctcat
     3121 gcgtcgctcg atccccgtca ctgctctcct tgtcagcctc gccctcggcg cctgcacgag
     3181 cggggccacg ccctcgccga gcagctccgc ggcgccgtcg gtcgccgcat cgaccgcgcc
     3241 aagtgttgcc cccagcgtcg ccccgagcgc gacgccggac gcctgcgcca aggacaacct
     3301 caagctcacg accg
//
LOCUS       DATAIW010000177        22782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_38718, whole genome shotgun sequence.
ACCESSION   DATAIW010000177 DATAIW010000000
VERSION     DATAIW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22782
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38718"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VHM48_05675"
     CDS             complement(<1..582)
                     /locus_tag="VHM48_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2754930.1"
                     /translation="MSQVQPPPPPASAARWLGTVRWVLPLGLAGIAAFIEWGEHIANG
                     EEEISAAFFGEVALFALVGPIAVAITLSWVVRVVEGYRATSAALESMNRSLEAIVAER
                     TSHLEAATAQLAAANDDLRQLDRLKSEFVSLVSHQLRAPLTNINGALEIVAQDADRLP
                     PGSRRTLQILTLESQRLSRLIQTILDVQRIDSGR"
     gene            845..1312
                     /locus_tag="VHM48_05680"
     CDS             845..1312
                     /locus_tag="VHM48_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2754931.1"
                     /translation="MFKKLALLGSMAILMAACGAAATASPSASPASKPAASVEESENP
                     TTSESPPAGARTVDLEIADGKITPATVPVKKGETVTFNAKNVSTVQVELIVGLKKDVD
                     ADSGDSLKEAEEIEAGASKTVTYTFDGDGPYAYGDQIADHYAKGAKGDIVLQP"
     gene            1399..2019
                     /locus_tag="VHM48_05685"
     CDS             1399..2019
                     /locus_tag="VHM48_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2754932.1"
                     /translation="MDDRRPGADRLPDGAPESVDPPVDDPPATAETGPRLSRRSFVLR
                     ALTVVGGGIAAILAAPVVGFVTAPGWRSSRAVRFLSTSVAPTLRSNDWTSVGKLLDFE
                     VGVPTYVLVDRPVVDGWAQETVPVGVQVVRQDEAEVAAFDPHCTHLGCPLAWSTGAET
                     FVCPCHGGSFNAAGEVLSGPPPRPMVRYETRVDAGEVLIGSLPEGA"
     gene            2023..3399
                     /locus_tag="VHM48_05690"
     CDS             2023..3399
                     /locus_tag="VHM48_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX2754933.1"
                     /translation="MHRIGRWVDERLNVSPLIAGFLDRKVPGNIGWFHTLGSATLFLL
                     IVQIATGIALSMSYVPSPDHAYQSILYIDQTPFGGAVRGIHHWAAGLLVLFIGLHILR
                     VFIWGAHRYPREITWLVGAGLFFIVLGFAFTGYLLPWDQKAYWATVVGTNIAGSAPLI
                     GGAVMEFLRGGEQLGAVTLARFYGIHIWVLPALLLVLVGAHLFGVIRQGIAASPRRHA
                     IVASDPDLSRREAYEREYAAEKRAGKPFWDSLLKDAVVAAILLVVVVALALTQGAPLE
                     PRADPNATNYVPRPEWYFLDMFQMLWYFTGDLEPLLIFLVFTFGAVIFLLIPFIDRGT
                     ARHPRQRPIAMGLTAVVVVAIVGLTYLGASATPAGVVKIPPTDGMAQAELNGLATFNE
                     QGCASCHQIRGLGGHTGPDLSRAGFRWEPAAIRDQIVTPKDTKMPSYDGLTQEQLDDL
                     VTYLTSLK"
     gene            3590..4882
                     /locus_tag="VHM48_05695"
     CDS             3590..4882
                     /locus_tag="VHM48_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754934.1"
                     /translation="MLSAGGAFDGSSSWRIAGHLRTLAIGVTLVLAIGCTATPAISPT
                     PNVGSASPAAGTQNPTPTSIAPTPSATDPVASWTRVPTTEALRGATMTRVVAGPAGLL
                     ALGSRDAGGFVSWWSGDGVGWTRSAYVGTAAAGGALALPDGFVILGRIGDGPDRERAM
                     TWRSSDGVQWVASPIDGLKTDPIAVGMVGGRIATFALRAPSGGEPTLAWSSSDLVTWA
                     SDVLGDGGYSDAAGITVLSDETGLAFGRWSAEPMDIAPFPPGQAAFWRSNDGRAWHRT
                     ADDPDLAAAVFVDVAQRSGSDVVAVGQRWDPAGQPDQAYAMALWSSSDGDAWDRVEVP
                     PALDPTAVPRRVVTTPTGYLLLASSPAAAPNALVASSTDGRIWTAFDPPIAFEGGQAN
                     DLAVVAGHLVAVGQTLPDGGTAADAAVWIGPTNAERTN"
     gene            complement(4908..5237)
                     /locus_tag="VHM48_05700"
     CDS             complement(4908..5237)
                     /locus_tag="VHM48_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754935.1"
                     /translation="MAHLGTWLAEAEEQLERITAGTYEGHEIDVDAVNATFLAAMAGQ
                     PWEVAWVQANAGRSRMVDEWSHLESPDDEAAWWIRKSGSDHYADHLDRLREWIAELIK
                     RRGGPRG"
     gene            complement(5412..5783)
                     /locus_tag="VHM48_05705"
     CDS             complement(5412..5783)
                     /locus_tag="VHM48_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2754936.1"
                     /translation="MDQPRPSAALGTEPIRTIDRDELKATLDRGDDVKLIMALNRWAF
                     EAKHIPGSIHFDTPEELYTAVRPDDDVIVYCSQVDCLSSVAVYRDLVRRGYRNVRRYS
                     GGLLDWEDAGLPLEGEMVGPS"
     gene            5971..6177
                     /locus_tag="VHM48_05710"
     CDS             5971..6177
                     /locus_tag="VHM48_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2754937.1"
                     /translation="MMNGLTMGLEGWLWMGVWVLVLVVTVWVLVREPHRRGRDEALDT
                     LRSRLARGEISRAEFEAARRLLES"
     gene            6203..6667
                     /locus_tag="VHM48_05715"
     CDS             6203..6667
                     /locus_tag="VHM48_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2754938.1"
                     /translation="MTTKAAARRAAQPTKTTLEKAGPAVFGLIVVAVLAVGGYLAFGD
                     FLNRPAAAAGSIDVQSSMAGFTPSEIRVKAGSTVTLDWWTQDASIHLKNGVHTMVAPD
                     LGLNESLPAESRRAVTWHVPNKPGTYDVYCDSCCGGKDSPTMHGKIVVEPAA"
     gene            6664..7521
                     /locus_tag="VHM48_05720"
     CDS             6664..7521
                     /locus_tag="VHM48_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2754939.1"
                     /translation="MSADGVPGRLPASPLLLGWPRPALALAALIAGVGSVALAYVLAV
                     PFNTAAFGGLTVPTVLVAGFADGFNPCAFALLVLFATYTLTLVNSVTSDGTPTLEARQ
                     RLLGAGSLYVAAVLITYFIIGLGLLSFLGWLGRDHLVSRGAAVLALFMALWMLKDVFL
                     PGVGPSMMAPGGTHGKMQKAMERGGLAGMLIAGVLVGICTVPCSGAIYLDITAVLHAS
                     GGGLTGVAMLALYNIAFIIPLLILLLAVSNRRVLGQLGRWNRANSPWIKAGLALVVIA
                     MSFGLMVSM"
     gene            7808..8386
                     /locus_tag="VHM48_05725"
     CDS             7808..8386
                     /locus_tag="VHM48_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2754940.1"
                     /translation="MTDDRTAGRAGIPPWTFLLIAIAALILATAALTATLIGGWNGGR
                     TTLMSSGGMMAGGMMPGGFANGAGGAGTANAPGPGGPGFVAGTSAAPRVIQVTAGPGY
                     AFRPSTISVARGETVTFLVTSMGPLVHEFMVGPADAVAADATGTPEVADITMMTSKSL
                     TFAFDGPGPYAFACHADGHYEAGMRGSILLVG"
     gene            8484..10646
                     /locus_tag="VHM48_05730"
     CDS             8484..10646
                     /locus_tag="VHM48_05730"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX2754941.1"
                     /translation="MSSPKPDENHDHRDASEHAGHPGEAAGSATVGEHGTHPAGHDVH
                     GAAGHDMSSSGHDMSSGGHDRHAGHSVAMFRDKFWLTLALTIPVVLLSADIQEWVGYR
                     IPTFPGIEYVPAIIGTIVFLYGGLVFVRGAQGELADRRPGMMTLISLAIVVAFVTSWA
                     GTLRLFEVEIWWELATLISIMLLGHWLEMRSIAQARGALSALAELLPDTAERVTADGT
                     EDVPIAALALGDIILVRPGARVAADGVVADGHADVDESMVTGESRAVAKEPGDSVVAG
                     TVAAGGSLRIRVTATGDATALSGIMRLVAAAQASASRAQALADRAAAILFYVALAAGT
                     ITLVFWWLQGDPEGALVRTATVLVIACPHALGLAIPLVIAISTSLGARNGLLVKDRLA
                     LERARTLDTVIFDKTGTLTRGAPALVDASDPEALRLAAAVEADSEHPLARAIVRGAEA
                     RNLAVPTATAFEALAGRGARARVDGHEVSVGGPRLLADLSLATLPVAADWDREGRTVL
                     HVIVDGRVAGVVAVEDEIRPESREAIDALHALGVRVAMITGDSQAVADSVARRLGIDE
                     VAAQVLPADKADAVRRFQQGGRRVAMVGDGVNDAPALATADVGIAIGAGTDVAVESAG
                     IVLVRSDPRDVVGAIELSRATYSKMIQNLIWATGYNLVAIPVAAGLFVPWGLDLPMAV
                     GAIAMSLSTIIVAANAQLLRRLELRRDPATAQPQLATA"
     gene            complement(10678..10896)
                     /locus_tag="VHM48_05735"
     CDS             complement(10678..10896)
                     /locus_tag="VHM48_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754942.1"
                     /translation="MYIVAPAFLVAGAILLARLPRPAGARIGLVVLVVALVGNVGLLL
                     ASHDRLVSKIECERSMTPLARGSAGNPC"
     gene            complement(11118..12008)
                     /gene="ppk2"
                     /locus_tag="VHM48_05740"
     CDS             complement(11118..12008)
                     /gene="ppk2"
                     /locus_tag="VHM48_05740"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX2754943.1"
                     /translation="MARGKAKHDRADGTGAAAGTGMPQPTEEGPVEVRPERPERMSNR
                     VYLAELAKLQVELATLQEWVRRKGLKVVVVFEGRDAAGKGGAIKTITLALNPRIARVV
                     ALPAPTERERTQWYFQRYVAHLPSAGEIVMFDRSWYNRAGVERVMGFATAEEVQEFYR
                     SCPEFERMLVRSGILLVKYWFSVSDGEQERRFQARIADPTKRWKLSPMDLQSRARWVD
                     YSMAKDDMFRATDIKQAPWHVVNADDKKRARLNLISHFLSLIPYEDLASEAFVLPPRE
                     PDRGYVRPPLDEQTFIPEVY"
     gene            complement(12150..13190)
                     /locus_tag="VHM48_05745"
     CDS             complement(12150..13190)
                     /locus_tag="VHM48_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2754944.1"
                     /translation="MGEGTIRTLGPLRSGTPWIVAVAVAVGLIACSSGPPAPTSSAFQ
                     PVASAAVSPQATPSSSGSIATPSAQPIPTPTSATPRPTSSADPSPTGTATTRPTTRPT
                     TRPTPDPTTAPAPTPTARPTPHPTAIPTPPPASSKVLGHGSRTSRNVALTLDMGGRVG
                     DALAIVDWLVDHDVHASIFMTGAMADNPNTDAGRTVLELIEAHPDLFTLGNHSYTHRD
                     FRNLTAAEIRDELRRTETALAARCTQDPRPFFRPPNGGYDADVLAAVGAAGYRYTVTW
                     DIDTIDWRPIANDPPGPTADQIVTKVLTRAQGGSIVLMHLGGYETFEALPRIVDGLGA
                     AGFDLVTLDEMF"
     gene            13415..14278
                     /locus_tag="VHM48_05750"
     CDS             13415..14278
                     /locus_tag="VHM48_05750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2754945.1"
                     /translation="MRSSLRHATVAALLFAFIAAGTASAAPGTSGDHSRSYYVSVGDS
                     LAAGVQPIGDPADLYRTDAGYAEQLLGIAQPQSPKLGLVKLGCPGETTTTMIAGGICS
                     YAHGSQLDEAVAFLRAHRAHVAFVTIDIGANDFPCQTAECVPAGVAAIQSNLPGILAQ
                     LRAAAGPTMPIVGMTIYNPFLAYWFAGPDGQAFARASASQLLGPVNALLRGIYEGSGD
                     GVADIERAFSSNDFDTLVDLPGAGTVPLNVARICMWTWVCAPAPYGPDNHANADGYAV
                     IARAYADVLGL"
     gene            14733..15026
                     /locus_tag="VHM48_05755"
     CDS             14733..15026
                     /locus_tag="VHM48_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2754946.1"
                     /translation="MTIATATDRTDARIEPRGPEFEFMPLEIAVDRCKGCGLCVDVCP
                     KHVLELDSTLVNPLGYHPVRLTDAAACTSCALCARICPDAVYTVFARPKGGMR"
     gene            15023..16387
                     /gene="vorB"
                     /locus_tag="VHM48_05760"
     CDS             15023..16387
                     /gene="vorB"
                     /locus_tag="VHM48_05760"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX2754947.1"
                     /translation="MTASDSVTTNRADPPAIADSALESGPGGTARPVLMKGNEAMAEA
                     AIAAGCDAYFGYPITPQAELLEVMAKRMPELGRVFVQAESELGAINMGLGAAATGARV
                     LVSSSSPGISLMAEGMSYLAGSELPLVLINVMRGGPGLGSIGPSQSDYFQATKGHGHG
                     DYRVPVLAPSTIAEAVALLADAFEIAERYRTPVMLLPDGVIGQAMEPVLPTWRTPPRI
                     KNDWELTGAAGRAPRVVRSLHLRPEDLEAHNTRLQAKYATIAEREVRWATESLDDAEI
                     LVVAYGTAARVARTAIERARRAGLAVGLLRPITLWPFPTAALAAIAPRMRAVVVVELS
                     AGQMIEDVRLAVEGRAPVFFHGRTGGMIPMPGEVLRAIARAWAMTTVRPDHRVEDSGL
                     AADTFAEEIVRAGDVVRAAAEPDPLSLIELATWAEVEGPIPTPELIEDPLDLVLAGHP
                     EMRR"
     gene            16393..17229
                     /locus_tag="VHM48_05765"
     CDS             16393..17229
                     /locus_tag="VHM48_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2754948.1"
                     /translation="MTSVAETRRIVYRPPQLLADHATHYCPGCGHGIVHRLIAELLEE
                     MDLADRTIGVASVGCSVFAYDYLAVDFVESPHGRAPAVATGVRRARPDAFVFTYQGDG
                     DLAAIGTAEIVHAAARGENISVIFVNNGIYGMTGGQMAPTTLLGQRTTSSPGGREASL
                     MGYPIPIAEMLAILPGVAYAARGTIADPGWIGKTKAMLRRSFEVQIAGAGLSIVEILS
                     TCPVGWGMTPAESMAHVTRDVVGTYPPGVIVDRLKAAADAAAVAATDAPPAPVAGSAG
                     GS"
     gene            17230..17832
                     /locus_tag="VHM48_05770"
     CDS             17230..17832
                     /locus_tag="VHM48_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2754949.1"
                     /translation="MMQQSTIFGGFGGQGLLFAGHVLAQAAVIEGKDVSWMPSYGPEM
                     RGGTASCTVIVSDEPIGSPIVDAADAVVALNPPSMAKFENLLVPGGILVLNASLIEAE
                     PRRTDIQIVPVACSAIARAAGDDRLVSVAALGALIAQRPIVTAAAIRQALADLIGHRP
                     ALLAADIVAFDRGAADAALLAATPVPRASTNGRGIADSTV"
     gene            18003..18521
                     /locus_tag="VHM48_05775"
     CDS             18003..18521
                     /locus_tag="VHM48_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2754950.1"
                     /translation="MTTTTTLDRPDQHLLDWLADHGIDHEVHPHERTVTAAATATAEG
                     VDPRTFAKVIAVATDDGRTVLLVVEASDRLDLDRARLATASSDVRLLDEIELAELAPD
                     CEVGALPPVGPLYGLSTVADHRLREDPEISFNAGSHRFAVRVERAAWERACSVIYANL
                     AADRDAGPAWAR"
     gene            18518..19255
                     /locus_tag="VHM48_05780"
     CDS             18518..19255
                     /locus_tag="VHM48_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754951.1"
                     /translation="MTRSGAAAPAHDPADATASLVWVDARQAIVLRWERGATTVTRRV
                     SDVPGHHHSTGHVRHDPSPGSGIAGPPRAAGETHRLEHLARFIEDVASALPTDGDVMV
                     IGPGTVHERLADRLHVLDVRHGGHRSVEREASEPLTERQLIARLRTLAGSPKPRRIHA
                     NPARVTVPARRASGSPARRPGRGFDPGIRRRPEAGFDEEPFGDDEPTEDLEGAGLVDA
                     DRVVTAGGAPGGAVVVSGADPGSPRST"
     gene            complement(19349..19897)
                     /locus_tag="VHM48_05785"
     CDS             complement(19349..19897)
                     /locus_tag="VHM48_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX2754952.1"
                     /translation="MTVLVTVASRHQATLEIGQAIGRALAASGIPTDVVPAEEIDAVT
                     PYEAVIIGSGVYAGNWLAPARDLIARCAMQLRERPVWLFSSGPMGDPPVPAGEPAGVA
                     ALKELIGPRGHRVFPGRLASGDLGFGEKLIVKAVRAPFGDYRDWPAIDAWAVEIAATL
                     RPAGVPEPSNPVPVGAATPGGR"
     gene            complement(19924..20130)
                     /locus_tag="VHM48_05790"
     CDS             complement(19924..20130)
                     /locus_tag="VHM48_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754953.1"
                     /translation="MKRLLGAVLAGFVALTWAVPVLAAGEVLAHAGRVLVAVGGDLVV
                     PAGEQVRGVSTSTLFRPTTAPMAR"
     gene            20353..21063
                     /locus_tag="VHM48_05795"
     CDS             20353..21063
                     /locus_tag="VHM48_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2754954.1"
                     /translation="MLIIRNVMSQPALTVHPTTPLKDVARLLVEHRISGLPVVDEGGR
                     VLGVVSEADLLLKEQGPEHVSHRHFARLLGESTETRAQLAKLDATTAGDAMSAPAITI
                     TGDRPIAEAAALMTSRGINRLPVVDDGTLVGVVSRGDLISAYVRSDEDLGDVVRNEVL
                     HRTMWLDPGLFEVVVQDGIVRIGGSVGRRSTAEVIERMTAMIPGVIDVISELTWETDD
                     RDLVAPERDLVSAYTLGR"
     gene            complement(21082..21795)
                     /locus_tag="VHM48_05800"
     CDS             complement(21082..21795)
                     /locus_tag="VHM48_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754955.1"
                     /translation="MADRTIAVDPRQVDAPTPADAFRTLTQLGFLAHSDLPDQAGSAY
                     LLVALRDRPALTHYDPEAVDYWITRDGVGRREQLTRTTPLPIADRPFAWGEIRIVDRL
                     HVGNEYLTFGGRLSAALVDGVAIAVFTSTVPLLRRGGHSQGWDEAAENVGAFFGRAKV
                     AVDYVPGFEARLAALRPEARYSAFIEDLADRYRGEPLLRETQSHLWSLVMAEEHRLAR
                     ASAGDLQEGRSLLAEMRAA"
     gene            21874..22248
                     /locus_tag="VHM48_05805"
     CDS             21874..22248
                     /locus_tag="VHM48_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754956.1"
                     /translation="MPILTFGGAGPPPDSATGRTAPPFRALRLVNGARATSTIDPALE
                     VLDMGGNRFTRSPRPRTEHLIALTVEVGQVVCPARGITDIEHCFTCSAYRGLQDGPAE
                     RLVCAPVTRSELAYVPLGFVPR"
     gene            22451..>22782
                     /locus_tag="VHM48_05810"
     CDS             22451..>22782
                     /locus_tag="VHM48_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754957.1"
                     /translation="MTGWTVLVCGDRSRGDDGAAIAAVERLPRRLRAEIRIRARSQLE
                     PDELVSVLLSGPCLILDTVHGVPPGTVVEVSLAKLLAGDGPTPASTHALPMPIVVGLA
                     RALGAPLET"
BASE COUNT         3255 a         8157 c         7918 g         3452 t
ORIGIN      
        1 cctgccggaa tcgatccgct ggacgtcgag gatcgtctgg atgaggcgtg acaggcgctg
       61 actctcgagc gtgaggatct ggagcgtgcg tcggctgccc ggcggcaggc ggtccgcgtc
      121 ctgggcgacg atctcgagcg ctccgttgat gttggtcaag ggcgcgcgga gctggtggga
      181 gacgagggat acgaactcgg acttcaaccg atcgagctgg cggaggtcgt cgttcgccgc
      241 ggcgagctgg gccgtggcag cctcgagatg gctggtccgc tcggccacga tggcctccag
      301 cgatcgattc atcgactcga gggccgctga tgtcgcccgg tacccctcga cgacgcgaac
      361 gacccagctg agggtgatcg cgaccgcgat cggtccgacc agggcgaaca gggcgacctc
      421 gccgaagaac gccgcgctga tctcctcctc gccgttggcg atgtgctcgc cccactcgat
      481 gaacgccgca atcccggcaa ggccgagggg caggacccag cgcacggtgc cgagccagcg
      541 cgccgcggag gccggtggtg gtggcggctg gacctggctc atgcccgagg gatctcctgc
      601 cgtgggccgc gattcccgag gtcggcggcg acgccgtggc ggtcgttgcg aaccacttct
      661 agcacaaggg cgggcgcggc acccccgtcg gcggtcgttc tcgacacaaa cgcgaccgcc
      721 gggcgaccgg atgaggacgg accggctcgt accttcgaac ccggagatgc gtgaagcgtc
      781 cgtcactcga cgagacgacc gcggtcgtgg ccgagtccac gggacgaaag gagacctcag
      841 accgatgttc aagaagctgg ccctgctcgg gtcgatggcg atcctcatgg ccgcctgcgg
      901 cgcggccgcc acggcgagtc cgtccgcgag cccggcgtcg aagccggcgg cgagtgtgga
      961 agagtcggaa aacccgacga cgtcggagtc gccgccggcc ggggcgcgga ccgtcgacct
     1021 cgagatcgcc gacggcaaga tcactcccgc cacggtcccc gtgaagaagg gcgagaccgt
     1081 cacgttcaat gcgaagaacg tgagcaccgt ccaggtcgag ttgatcgtcg gcctgaagaa
     1141 ggacgttgac gcagattccg gcgacagcct gaaggaggcc gaggagatcg aggcgggggc
     1201 ctcgaagacc gtgacctaca ccttcgacgg cgacgggccg tacgcgtacg gcgatcagat
     1261 cgccgaccac tacgcgaagg gcgccaaggg agacatcgtc ctccagccct gaacgtcgca
     1321 ctggatccgg gtccccgggg acgacggctc gacgggggtc gttgtccccg atcgcattcg
     1381 cgaaggaggc aggctgccat ggacgaccgt cgacccggtg cggaccggtt gcccgacggt
     1441 gcgccggagt cggtcgatcc gcccgtcgac gacccgcccg cgaccgccga gaccggaccg
     1501 cgcctgtcga ggcgcagctt cgtgcttcgg gcgctgacgg tggtcggagg cgggatcgcc
     1561 gcgatcctcg cggccccggt cgtcggcttc gtgaccgcgc ctggctggcg ctccagccgg
     1621 gccgtgcgat tcctctcgac atcggtcgcg ccgacgctcc gctcgaacga ctggacctcg
     1681 gtcgggaagc tcctcgactt cgaggtcggt gtgccgacct acgtcctggt cgaccgaccg
     1741 gtggtcgatg gctgggccca ggagaccgta ccggtcggcg tccaggtcgt ccggcaggac
     1801 gaggcggagg ttgccgcgtt cgatcctcac tgcacgcatc tcggatgccc gctcgcctgg
     1861 tcgaccgggg ccgagacctt cgtgtgcccc tgtcacggcg gctcgttcaa cgcggcgggc
     1921 gaggtgctct ccggtccgcc gccgcggccg atggtccgtt acgagacccg agtcgacgcc
     1981 ggcgaggtgc tcatcggctc attgcccgaa ggagcctgaa ccgtgcaccg catcggacgc
     2041 tgggtcgacg agcgactgaa cgtctcgccg ctgatcgcgg gattcctcga tcggaaggtc
     2101 cccggcaaca tcggctggtt ccataccctc ggcagcgcga ccctgttcct cctgatcgtc
     2161 cagatcgcga ccggcatcgc gctctcgatg agctatgtcc cgagcccgga ccacgcgtac
     2221 cagagcatcc tctacatcga ccagacgccg ttcggcggcg ccgtccgcgg gatccaccat
     2281 tgggcggcgg ggctgctcgt cctgttcatc ggactgcaca tcctgcgggt cttcatctgg
     2341 ggcgcccatc gctatccccg cgagatcacc tggctcgtcg gcgccggcct gttcttcatc
     2401 gtcctcggct tcgccttcac gggctacctc ctgccgtggg atcagaaggc ctattgggcg
     2461 accgtcgtcg gaacgaacat cgccggttcg gcgccactga tcggcggggc ggtcatggag
     2521 ttccttcgag ggggcgagca gctcggggcc gtgaccctgg cccggttcta cggcatccac
     2581 atctgggtgc ttccagccct cctgctggtc ctcgtcggag cgcacctgtt cggcgtgatc
     2641 cgccagggga tcgcggcctc acctcggcgc catgcgatcg tcgccagtga tccagacctc
     2701 tcccgccgcg aggcgtatga acgcgagtac gcggccgaga agagggccgg caagccattc
     2761 tgggactccc tcctcaagga cgcggtggtg gccgccatcc tgctggtcgt cgtcgtcgcg
     2821 ctcgcgctga cccagggcgc accactcgag ccgcgggctg acccgaatgc cacgaactac
     2881 gtgccacgtc cggagtggta cttcctcgac atgttccaga tgttgtggta cttcaccggc
     2941 gacctcgaac cgctgctgat cttcctggtc ttcacgttcg gcgcggtcat cttcctgctg
     3001 atcccgttca tcgaccgcgg aacggccagg catccccgcc agcgacccat cgccatgggc
     3061 ctgaccgcgg tggtcgtcgt cgcgatcgtc ggtctgacct atctcggggc gtcagccacc
     3121 ccggccggcg tcgtgaagat cccgccgacc gatggcatgg cccaggcgga gctcaatggc
     3181 ctggcgacgt tcaacgagca gggctgtgcc tcctgccacc agatccgcgg cctcggcggc
     3241 cacacgggtc cggacctgtc gcgagccggc ttccgttggg aacccgctgc gatccgtgat
     3301 cagatcgtca cgcccaagga cacgaagatg ccgagctacg acgggctgac gcaggagcag
     3361 ctggacgacc tggtcaccta cctcaccagc ctgaagtagg ccacacgcca ggggcagccg
     3421 gacggcgttg gagaggacct ccagcgcgcc gccggcgccg cgatcgctta cgacgtgatc
     3481 gtcggacgaa tttgtcgact tccggcactc gtcaggggct ccccgctggg tcaccgttcg
     3541 gggggttacc tgaatggatc gcgcgggtgc gcgacggagt cggttcctca tgctctccgc
     3601 gggcggagcg ttcgacggat cctcgagctg gcggatcgcc ggccacctga ggacgcttgc
     3661 catcggcgtg accttggtgc tcgccatcgg ctgcaccgcc acgcccgcca tctcccccac
     3721 cccgaacgtc ggatcggcga gccccgctgc gggcacgcag aatccgacgc cgacatcgat
     3781 cgcgcccacg ccctcggcga ccgatcccgt cgcgagctgg acgcgggtac cgacaaccga
     3841 agccctgcgc ggcgcgacga tgacccgggt ggtggccggg ccggccgggc tgctcgcact
     3901 cgggagccgc gacgcggggg gattcgtcag ctggtggtcg ggggatggcg tcggatggac
     3961 ccggtccgcg tacgtcggca cggcggccgc gggcggcgcc cttgccctcc ccgatggctt
     4021 cgtcatcctc ggcaggatcg gcgacggccc cgaccgcgag cgggcgatga catggcgatc
     4081 gtcggatggg gtccaatggg ttgcctctcc catcgatgga ctcaagacgg acccgatcgc
     4141 cgtcggaatg gttggaggcc ggatcgcgac cttcgcgctg cgagcgccga gtggcggaga
     4201 gcccaccctg gcctggtcga gcagcgatct tgtcacctgg gcgtccgatg tcctcggcga
     4261 tggcggctac tcggacgcgg ccggcatcac ggtcctgtcc gacgagaccg gtctcgcgtt
     4321 cggtcgctgg tcggcggaac cgatggacat cgctccgttc ccgccgggcc aggcggcgtt
     4381 ctggcggtcg aacgatggcc gggcctggca caggacggcc gatgacccgg atctcgccgc
     4441 ggccgtgttc gtcgacgtcg cccagcggtc cgggagtgac gtcgtcgcgg tcggccaacg
     4501 ttgggatcct gccgggcaac cggatcaggc gtacgccatg gccctctggt cctcgtctga
     4561 tggcgacgcg tgggaccgcg tggaggtgcc gccagccctg gatccgacgg cggtcccgag
     4621 gcgggtcgtg accacgccga cggggtatct cctcctcgcg tcatcacccg ccgcggcgcc
     4681 gaatgccctg gtcgccagct cgaccgatgg gcgcatctgg accgccttcg acccgccgat
     4741 cgcgttcgag ggcggccagg ccaacgacct cgcggtcgtt gccggacatc tcgtcgccgt
     4801 cggacagacg ctgccggacg gcggaacagc ggccgacgcc gccgtgtgga tcgggccgac
     4861 caacgcggag cgaacgaact gacagaagcg tgagcggcgc gggaccgcta gccccgcggt
     4921 ccaccgcgtc gtttgatcag ctcggcgatc cactcgcgaa ggcgatcgag gtggtcggcg
     4981 tagtggtccg agccggactt gcggatccac cacgccgcct cgtcgtcggg cgattcgagg
     5041 tgcgaccact cgtcgaccat tcgtgagcgg cccgcgttcg cctggaccca ggcgacctcc
     5101 cacggctgtc cggccatggc cgccaggaac gtcgcgttca cggcgtcgac gtcgatctcg
     5161 tggccctcgt aggtcccggc ggtgatccgc tcgagttgct cctcggcctc ggcaagccag
     5221 gtgcccagat gggccacgac atcgcgaacg gtccagtccg ggtcgcggta gtagcccggg
     5281 gatcaggcac tcggcagggg tcagcgagcg caccaggctc gcgacctcgt accagccggc
     5341 acgttccgcc tcgagctcgg cggggtaggg ccgatcgcgc ggccggtcga ccggcgtcgg
     5401 ctcgctcgcc gtcatgacgg gccgaccatc tcgccctcga gcggcaggcc ggcatcctcc
     5461 cagtcgagca accccccgga gtaccgccgc acgttccgat agcctcgacg gaccaggtcg
     5521 cggtagacgg cgacgctcga gaggcagtcg acctgcgagc agtacacgat gacgtcgtca
     5581 tccggtcgaa cggccgtgta gagctcctcg ggcgtgtcga aatggatcga cccggggatg
     5641 tgcttcgcct cgaacgccca gcgattgagg gccatgatca gcttgacgtc gtcgccccga
     5701 tcgagcgtcg ccttgagctc gtcgcgatcg atcgtccgga tcggttcggt cccgagggca
     5761 gcgctcggac ggggctggtc catggtcgcc atgatgctcg ccctcgagcc acccgtcgac
     5821 cgtcgcgacg cgtgcttgcc actcgctcga ccggacgtca gcttgaccgc gtcttgagga
     5881 actcttgccc attccctcac ggacgcctga cgttcgcgag cgaacctccc catacggccg
     5941 gccgcgtccc ggcctcacgg gaggtctccg atgatgaacg gtctcacgat ggggctcgag
     6001 ggatggctgt ggatgggagt ctgggtgctc gtcctcgtcg tcaccgtctg ggtcctcgtg
     6061 cgcgaacccc atcgacgcgg gcgcgatgag gcgctcgaca ccctccgttc ccgacttgcc
     6121 cgcggcgaga tcagccgcgc tgagttcgag gcggcccggc gccttctcga gtcctgaaca
     6181 cgcctggaag gagtcgaccc cgatgacgac gaaggctgct gcgcggcggg ctgcgcagcc
     6241 gacgaaaacc acgctcgaga aggcggggcc cgcggtcttc gggttgatcg tcgtcgccgt
     6301 cctcgccgtc ggcgggtacc tggcgttcgg ggacttcctg aaccggccgg ccgcggcggc
     6361 cgggtcgatc gacgtccaga gctcgatggc cgggttcacc ccgtccgaga tccgggtcaa
     6421 ggccggatcg accgtcacgc tcgactggtg gacccaggac gcctcgatcc atctcaagaa
     6481 cggcgttcac acgatggtcg cccccgatct cgggctcaac gaatcgctcc cggccgagag
     6541 ccgcagggcg gtcacctggc acgtcccgaa caagcccggc acgtacgacg tctactgcga
     6601 ctcgtgctgc ggcggcaagg acagcccgac gatgcacggc aagatcgtgg tggagcccgc
     6661 ggcatgagcg ccgacggggt cccagggcga ctgcccgcgt cgccgctgct cctcggctgg
     6721 ccacggccgg cgctcgcgct cgcggccctc atcgccggcg tcgggtcggt cgcgctcgcc
     6781 tacgtgctcg cggtcccgtt caacaccgca gcattcggcg gcctgaccgt cccgaccgtc
     6841 ctcgtcgcag ggttcgccga tgggttcaac ccatgcgcgt tcgccctgct cgtcctgttc
     6901 gcgacgtaca cgctgaccct ggtcaactcc gtcacctccg acgggacgcc aaccctcgag
     6961 gcgcgccagc gcctcctcgg agcaggatcg ctctacgtgg cggcggtcct gatcacctac
     7021 ttcatcatcg ggctcggcct gctgagcttc ctcggctggc tcgggcgcga ccacctcgtg
     7081 tcacgcgggg cggcggtcct cgccctgttc atggccctgt ggatgctcaa ggacgtgttc
     7141 ctgcccggtg tcgggccgtc gatgatggcc cccgggggaa cgcacgggaa gatgcagaag
     7201 gccatggaac gcggcggtct cgccgggatg ctcatcgcgg gcgtcctcgt cgggatctgc
     7261 acggttccct gctcgggcgc catctacctc gacatcaccg cggtccttca cgcctcgggt
     7321 ggcggactca ccggcgtcgc gatgctcgcg ctgtacaaca tcgcgttcat catcccgctt
     7381 ctgatcctcc tgctcgcggt gagcaatcgg cgggtcctcg gccagctcgg ccgctggaac
     7441 cgggcgaact cgccctggat caaggccggc ctggccctcg tcgtcatcgc gatgagcttc
     7501 ggcctgatgg tgtcgatgtg agcgagatcg agacgatccg cttcccggtg gtggggatga
     7561 cgtgcgcgtc atgcgtcaac cggatcacgc ggtctctacg aagggtggaa ggggtgtcga
     7621 gcgtcaaggt cgatctcggc cgggagaccg tgaccgtggg ccgagacccc ggtcgggtgg
     7681 ccgatggcgc actcgccgcc gcgatcgccg acgcgggcta ccaggccgac gtcgctgccg
     7741 ccgtgcccgt cgccggcagt cccggcgcgg cgtcgaggct cgtcgaccgc ttacggagga
     7801 gaactcgatg acggacgacc gaacggccgg ccgggccggc attccgcctt ggaccttcct
     7861 cctgatcgcg atcgcggcgc tcatcctcgc gaccgctgcc ctgaccgcga cgctgatcgg
     7921 cggctggaac ggaggccgca ccaccctcat gtcgagcggc ggcatgatgg ccggcggcat
     7981 gatgcccggc ggattcgcga acggcgcagg tggcgcgggc accgcaaacg cccctggtcc
     8041 gggcgggccc ggcttcgtcg ccggaacctc ggcagcgccg cgcgtcatcc aggtcactgc
     8101 cggtccgggc tacgcgttcc gtccgtcgac gatctccgtc gctcgaggcg agaccgtcac
     8161 gttcctcgtg acgtcgatgg gcccgctggt ccacgagttc atggtgggtc cggccgacgc
     8221 ggtcgccgct gatgcgacgg gcacgccgga ggtcgccgac atcacgatga tgacgtcgaa
     8281 gtccctgacg ttcgccttcg acgggcccgg gccatacgcc ttcgcctgcc acgccgacgg
     8341 ccactatgaa gccgggatgc ggggttcgat cctcctggtc ggatagtttc cgcgacctcg
     8401 tgcggtggcc ggcgcgaagc actcccttgc gctcgaaggc cgggcgtgca acgatactcc
     8461 ccgggagtat tggaatggac cacgtgagca gcccgaagcc ggacgagaac cacgaccatc
     8521 gggatgcctc cgagcacgca ggccatcccg gggaggcggc cgggagcgcg acggtcggtg
     8581 agcacggcac gcacccagcc ggccacgacg tgcacggcgc cgcgggccac gacatgagct
     8641 caagcggcca cgacatgagc tccggcggcc acgaccgcca cgctggccat tccgtcgcga
     8701 tgttccgcga caagttctgg ctgaccctcg ccctgaccat ccccgtcgtc ctgctgagtg
     8761 ccgacatcca ggagtgggtc ggctacagga tcccgacgtt cccgggcatc gaatacgtcc
     8821 cggccatcat cggcacgatc gtcttcctct acggcgggct ggttttcgtc cgcggcgccc
     8881 agggcgagct ggccgaccgc aggcccggga tgatgaccct gatctcgctg gcgatcgtcg
     8941 tcgccttcgt cacctcgtgg gccggcacgc tccgcctgtt cgaggtcgag atctggtggg
     9001 aactggcgac cctgatcagc atcatgctgc tcggccactg gctcgagatg cgctcgatcg
     9061 cccaggcccg gggcgccctc tcggccctcg ccgagctcct gccagacaca gcggaacggg
     9121 tcaccgccga cgggacggag gacgtcccga tcgcggccct cgctctgggc gacatcatcc
     9181 tggtgcgccc cggcgcaagg gtcgccgcgg atggcgtggt cgcggacggc catgcggatg
     9241 tcgacgagtc gatggtcacc ggtgagtcgc gggcggtcgc caaggaaccc ggcgactcgg
     9301 tggtggctgg gaccgtggcg gccggcggct cgctgcgcat ccgggtgacc gcgaccggcg
     9361 acgccacggc tctatcagga atcatgcgcc tggtcgcggc cgcgcaggcg tcggcctcgc
     9421 gtgctcaggc cctggccgac cgggcggcgg ccatcctgtt ctacgtcgct ctcgcggcgg
     9481 gcacgatcac gctcgtgttc tggtggctcc agggtgaccc ggagggcgcc ctggtccgca
     9541 ccgcgaccgt cctggtcatc gcctgcccgc atgccctcgg actggcgatc ccgctggtca
     9601 tcgccatctc cacgtcactc ggcgcccgca acggcctcct cgtcaaggac cggctggccc
     9661 tcgagcgggc ccgcaccctc gacacggtca tcttcgacaa gaccgggacg ctcacgaggg
     9721 gggcgccggc cctcgtggac gccagcgatc cggaggcgct ccgtctcgcg gccgccgtcg
     9781 aggccgactc ggagcacccg ctcgcccggg cgatcgtccg cggggccgag gctcggaacc
     9841 tcgccgtccc gaccgccacc gccttcgagg cgttggccgg ccgcggagcc cgggcccgcg
     9901 tcgacggcca cgaggtcagc gtcggcgggc cgcgcctcct ggcagacctg tcgctcgcca
     9961 ccctgccggt cgccgccgac tgggaccgcg agggccggac ggtgcttcat gtgatcgtcg
    10021 atggccgggt cgccggggtg gtcgcggtcg aggacgagat tcgtccggag tccagggaag
    10081 cgatcgatgc gctccatgcc ctcggcgtcc gcgtcgccat gatcaccggc gacagccagg
    10141 ccgtggccga ctcggtggcg cgccggctgg gcatcgacga ggtcgcggcg caggtgctcc
    10201 cggccgacaa ggccgacgcg gtgcggcgct tccagcaggg cggccgcagg gtggcgatgg
    10261 tcggggacgg cgtgaacgac gcgccggcgt tggcgacggc cgacgtcggc atcgccatcg
    10321 gcgcgggcac cgacgtggcg gtcgagtcgg ccgggatcgt cctcgtccgg agcgatcccc
    10381 gggatgtggt cggcgcgatc gagctgtccc gggcgaccta cagcaagatg atccagaacc
    10441 tcatctgggc gaccggctac aacctggtcg ccatcccggt ggcggccggg ctgttcgtcc
    10501 cctggggcct ggatctgccg atggccgtcg gcgccatcgc gatgagcctc tcgaccatca
    10561 tcgtcgcggc caacgcccag ctccttcgcc gcctcgaact ccggcgcgat cccgcgactg
    10621 cccaaccgca gctggcgacc gcctgagtcc accgggggcc agggcgatcc cggccgatca
    10681 gcaggggttg ccggcgctgc cgcgggcgag cggcgtcatc gaccgctcgc actcgatctt
    10741 cgagacgagg cggtcgtggc tcgcgaggag caggccgacg ttcccgacga gggccacgac
    10801 caggacgacc agcccgatcc gggcgccggc cggtcgtggc aggcgggcga ggaggatcgc
    10861 accggccacc aggaacgccg gggcgacgat gtacacgtag cgcggggctg ccgcctgcgc
    10921 ttgcggccat ccagtggcaa ggcagagccg tctcgagcag catgccgctt ccggtcagga
    10981 ccaggcccgc ggggccggac aggccgacgg cgtcgacata ttcgggccgt gtcgcgccac
    11041 ggttacccag cagacggctg gaggttcctc gccggatcca tctgccactg tccgcccgcc
    11101 gttcggtcgg atgtgcctca gtagacctcc gggatgaacg tttgctcgtc gagcggcggc
    11161 cggacatagc cacggtcggg ctcgcgcggc ggcaggacga acgcttcgct cgccaggtcc
    11221 tcgtacggga tgagcgagag gaagtggctg atcaggttga ggcgggcccg cttcttgtcg
    11281 tcggcgttga ccacgtgcca gggcgcctgc ttgatgtcgg tcgcccggaa catgtcgtcc
    11341 ttggccatcg agtagtcgac ccagcgggcc cgcgactgga ggtccatcgg gctgagcttc
    11401 cagcgcttgg tcggatccgc gatccgcgcc tggaagcgcc gttcctgttc gccgtcgctg
    11461 acggagaacc agtacttgac gaggaggatc cctgaccgga cgagcatccg ctcgaactcc
    11521 ggacagctcc ggtagaactc ctggacttcc tcggctgtcg cgaagcccat gacccgctcg
    11581 acgccggccc ggttgtacca gctgcgatcg aacatcacga tctctccagc gctcgggaga
    11641 tgggccacgt agcgctggaa gtaccactgg gtccgctccc gctccgtcgg tgccggcagg
    11701 gccacgaccc gcgcgatccg ggggttcaac gcgagcgtga tcgtcttgat cgcgccgccc
    11761 tttcccgccg cgtcacgacc ctcgaagacg acgacgacct tgagcccctt gcggcggacc
    11821 cactcctgga gcgtcgccag ctcgacctga agcttggcca gctcggcgag gtacacccga
    11881 ttcgacatcc gctccggccg ctcaggacgg acctcgacgg ggccctcctc cgtcggttgc
    11941 ggcatgcccg tgccggccgc ggcgcccgtt ccgtcggccc ggtcgtgctt cgccttgcct
    12001 ctcgccatgg ccacccctcg cgctatccgt cccgtcctgc tgcccgatga gtatgggcgc
    12061 gaagggagcg actcttcagc gacgccggca ggacctgcgg tcgagccgac tcgcctgccg
    12121 cggcgcctgc tcgggaggtc gcccgcgatt cagaacatct cgtcgagggt gacgaggtcg
    12181 aacccggccg cccccagccc gtcgacgatc cgcggcaggg cctcgaacgt ctcatagccg
    12241 ccgaggtgca tcagcacgat cgagccgccc tgcgctcgag tgaggacctt cgtcacgatc
    12301 tggtcggcgg tcgggcccgg cgggtcgttg gcgatcggtc gccagtcgat cgtgtcgatg
    12361 tcccaggtga ccgtgtaccg gtagcccgcc gcaccgaccg cggccagcac gtcggcgtcg
    12421 tagccgccgt tcggcggccg gaagaacgga cgcgggtcct gggtgcagcg ggccgccagc
    12481 gccgtttcgg tccgacgcag ctcgtcgcgg atctcggcgg cggtcaggtt ccggaagtcg
    12541 cggtgggtat aggagtggtt gccgagggtg aacagatccg ggtgcgcctc gatgagctcg
    12601 aggaccgttc ggccggcgtc ggtgttgggg ttgtcagcca tcgccccggt catgaagatc
    12661 gaggcgtgga catcgtggtc cacgagccag tcgacgatgg cgagcgcatc cccgacccga
    12721 ccgcccatgt ccagcgtcag tgcgacattc cggctcgtcc gtgagccgtg ccccaggacc
    12781 ttgctcgacg caggaggagg cgtcgggatc gccgtggggt ggggcgtggg ccgcgccgtc
    12841 ggcgttggcg ccggcgccgt ggtgggatcg ggcgtcgggc gcgtcgtcgg gcgcgtcgtc
    12901 gggcgcgtcg tcgctgtccc ggtcgggctc gggtcggccg acgaggtcgg acgcggcgtc
    12961 gcgctcgtcg gcgtcgggat cggttgggcc gacggcgtcg cgatcgagcc ggatgacgaa
    13021 ggcgtcgcct gcggcgagac cgcggccgac gcgacgggct ggaaggcgct ggatgtcggt
    13081 gccggtggcc cgctcgagca ggcgatcagc ccgaccgcga ccgcgaccgc gacgatccag
    13141 ggagttccgc tccgaagcgg cccgagcgtc ctgatcgtgc cctcgcccat tggcacacca
    13201 cccagatcag gctgccaccg cgatgcgaga tcgccaacgg ccggaggccc cgaatcctcc
    13261 ggccgaacgg cccgctcggt ctgccagcgc cgtgcgggcc cgacttgtcg cctcgccgac
    13321 aaccccgcaa cggcgttggt cgtcgggcgt cgcaggtacg ggagccatcc atactcgcgt
    13381 cgtcggcgcg gtgacggctc cagaggagct gcagatgaga tccagcctcc gccacgccac
    13441 cgtcgcggcg ctcctgttcg ccttcatcgc ggcgggcacg gcatcggccg cgcccggcac
    13501 gagcggcgac cattccaggt cgtactacgt gtccgtcggc gactcgctgg cggccggcgt
    13561 ccagccgatc ggcgatccgg ccgacctgta ccggacggac gctggctacg ccgagcagct
    13621 cctcgggatc gcccagccgc aatcgcccaa gctcggcctc gtcaagctcg gctgcccggg
    13681 cgagaccacc acgacgatga tcgccggcgg gatctgctcg tacgcccatg gatcgcagct
    13741 cgatgaggcc gtcgccttcc tccgcgcaca ccgcgcccac gtcgccttcg tgacgatcga
    13801 catcggcgcg aacgacttcc cgtgccagac ggccgaatgc gtcccggcgg gcgtggccgc
    13861 aatccagtcg aacctgccgg gcatcctcgc ccagctgcgc gccgcggccg gtccgaccat
    13921 gccgatcgtc gggatgacga tctataaccc gttcctcgcc tactggttcg ccggcccgga
    13981 cgggcaggcg ttcgcgcggg caagtgcaag ccagctcctc gggccggtga acgcgctcct
    14041 gcgcggcatc tacgaagggt ccggcgacgg cgtcgcagac atcgagcggg cgttctcgtc
    14101 gaacgacttc gacaccctcg tggacctgcc gggcgccggc acggttccac tcaacgtggc
    14161 ccggatctgc atgtggacct gggtctgcgc accggccccg tacggccccg acaaccatgc
    14221 caacgccgat ggctacgccg tgatcgcccg ggcgtacgcc gacgtcctcg gtctctgatc
    14281 cggcgtccgg taaccgagcg gaggttcagg acccgccacc cggaatgaca acgacggcgg
    14341 cgatggggtc atcgccgccg tcgagtcgtg cccggcggtg gacggcctgg gggttggacc
    14401 gtccgggaat gtgctgattg cgcgaccccc cgggatgcag cacgtctgct ggagatcggg
    14461 cccatcaaac gtcagcgccc tgatcggccg caggtgccga acggcccgac gccaccggcc
    14521 gtacgacccg agccgaccgt acccccctgc cccggcgatc gcggtgcctg gccggcgacc
    14581 gacgctctca ctctccgccc tcgggtcgcg ctatcgccgc cgccccggac ggcgttccgg
    14641 cccgcgatcg gctgaatggc ccgctcccga ccgccattca gcactgacgc ccgcggtccg
    14701 gccctcgcta aggtgcgagt ggaggtcgct tgatgaccat cgccaccgcg acggatcgca
    14761 ccgacgcgcg gatcgagccg cgcggtccgg aattcgaatt catgccgctc gagatcgccg
    14821 tcgatcggtg caagggttgc ggcctctgtg tcgacgtctg cccgaaacac gtgctggagc
    14881 tcgactcgac gctcgtcaac cccctcggct accacccggt tcgcctgacg gatgcggcgg
    14941 cgtgcacgag ctgtgcgctc tgcgcccgca tctgtcccga cgccgtctat accgtcttcg
    15001 ctcgacccaa agggggcatg aggtgaccgc ctccgacagc gtgaccacca accgagccga
    15061 tccgccggcg atcgccgact ccgcactgga gtcgggcccg ggcggcacgg cccggccggt
    15121 cctgatgaag ggcaacgagg cgatggcgga agcggcgatc gccgccggct gcgacgccta
    15181 tttcggctac ccgatcacgc cccaggccga gctcctggag gtcatggcca agcgcatgcc
    15241 cgagctcggg cgagtcttcg tccaggccga gagcgagctc ggcgcaatca acatggggct
    15301 cggagccgcc gcgaccggcg cccgcgtcct cgtctcgtcg tcgagcccgg gcatcagcct
    15361 gatggccgag ggaatgagct atctcgccgg gtcggagctg ccgctcgtcc tgatcaacgt
    15421 catgcgcggc ggcccgggtc tcggcagcat cggcccgtcg cagtcggact acttccaggc
    15481 gacgaagggc catggacatg gcgactatcg cgtgcctgtc ctcgcgccgt cgacgatcgc
    15541 cgaggccgtc gcactcctcg ccgatgcctt cgagatcgcg gagcgctatc ggacgccggt
    15601 catgctgctt cccgacgggg tgattggcca ggcgatggag ccggtcctgc ccacatggcg
    15661 aacgccgccg cggatcaaga acgattggga gctgaccggc gcggccggcc gggcgccgcg
    15721 ggtcgtccgc tcgctccacc ttcggccgga ggatctcgaa gctcacaaca ccaggctcca
    15781 ggccaagtac gcgacgatcg ccgaacgtga ggtccgttgg gccaccgagt cgctcgatga
    15841 cgcggagatc ctggtcgtgg cctacggcac cgcggcacgg gtggcgcgga cggccatcga
    15901 gcgggcccgc cgggcggggc tcgccgtcgg cctgctgcga ccgatcacgc tatggccctt
    15961 cccgacggcg gctctggcgg ccatcgcgcc gcggatgcgc gccgtggtcg tcgtggagct
    16021 ctccgccggc cagatgatcg aagacgtgcg cctcgccgtc gagggccgag cgccggtgtt
    16081 cttccacggt cggaccgggg ggatgatccc gatgccgggc gaggtcctcc gggcgatcgc
    16141 ccgggcgtgg gcgatgacga ccgtccggcc ggatcaccgg gtggaggact ccggcctggc
    16201 cgcggacacc ttcgccgagg agatcgtccg ggccggtgat gtcgtgcgtg cggcagccga
    16261 gccggatccc ctgagcctga tcgagctcgc cacctgggca gaggtcgaag gcccgatccc
    16321 gacgcccgag ctcatcgagg acccactcga tcttgtcctg gccggccatc cagagatgag
    16381 gcggtgaccg agatgacgag cgtcgccgag acccgccgga tcgtctaccg gccgccacag
    16441 ctcctggccg atcatgcgac ccactattgt cccggctgcg ggcacgggat cgtccaccgg
    16501 ctgatcgccg agctgctcga ggagatggac ctggccgatc ggacgatcgg cgtggcctcg
    16561 gtcggctgca gcgtcttcgc ctacgactat cttgcggtcg acttcgtcga atcgccccat
    16621 ggacgcgcgc cggcggtcgc gaccggtgtc cgccgggcgc ggcccgacgc cttcgtcttc
    16681 acctatcagg gtgacggcga cctcgccgcg atcggcaccg ccgagatcgt ccatgccgcc
    16741 gcccgtggcg agaacatcag cgtgatcttc gtcaacaacg ggatctacgg gatgaccggc
    16801 ggccagatgg ccccgaccac gctgctcggc cagcggacca cctcgagccc gggcggccgc
    16861 gaggcgtccc tgatgggcta cccgatcccg atcgccgaga tgctcgcgat cctgcccggc
    16921 gtcgcctatg cggcgcgtgg cacgatcgcg gaccccggct ggatcggcaa gacgaaggcc
    16981 atgcttcgcc gctcgttcga ggtccagatc gccggcgccg ggctctcgat cgtcgagatc
    17041 ctctcgacct gccctgtcgg ctggggcatg accccggcgg agtcgatggc ccatgtgacg
    17101 cgcgacgtcg tcggcaccta tccgccgggc gtgatcgttg atcgcctgaa ggccgccgcc
    17161 gatgctgctg ccgtggccgc caccgacgcc ccgcccgccc ccgttgccgg ctccgccggc
    17221 gggagctgaa tgatgcagca atcgacgatc ttcggcggct tcggcgggca gggcctgctg
    17281 ttcgccggtc acgtcctcgc ccaggcggcg gtcatcgagg gaaaagacgt ctcgtggatg
    17341 ccgtcgtacg gaccggagat gcgcggtggc acggcgtcct gcacggtcat cgtctcggac
    17401 gaacccatcg ggtcgccgat cgtcgacgcc gccgacgcgg tcgttgccct caaccccccg
    17461 tcgatggcga agttcgagaa tctgctcgtc cccgggggca ttctcgtcct gaacgcatcg
    17521 ctgatcgaag ccgagccgcg gcggaccgat atccagatcg ttccggtcgc gtgctccgcg
    17581 atcgcgcgcg cggccgggga cgaccgcctc gtcagcgtgg ccgcacttgg cgccctgatc
    17641 gcccagcggc cgatcgtgac cgcggctgcc attcgccagg ccctggccga cctcatcggg
    17701 caccggccag cactgctggc cgccgatatc gttgcgttcg accgcggcgc cgcggacgcg
    17761 gccctgctcg ccgccacacc cgtgccccga gcatcgacca acggccgagg gatcgccgac
    17821 tcgaccgtct gacccgtcca gttcgtgacc tccggccgat gcgctcgcgc catcgtcggc
    17881 ggatgctcgg tgcgtaccga tcgctcgaac tgtcgacagg actccgctcg tcgagacgtc
    17941 ccggtcgtcc cgttccgtgc ccgacccggc gagcgatcgc gcgactggag gttggtcacg
    18001 ccatgacgac gaccacgacc ctcgaccggc cagatcagca cctcctcgac tggcttgctg
    18061 accacggcat cgatcacgag gtccatccgc atgagcgaac ggtgacggcc gccgccaccg
    18121 cgacggcaga aggcgtcgat ccgcgaacct tcgcgaaggt catcgcggtc gccacggacg
    18181 acgggcggac ggtgcttctc gtcgtcgaag cgtccgaccg gctcgacctc gaccgcgccc
    18241 gcctggcgac ggcgtcgagt gacgtccggc tcctcgacga gatcgagctc gcggagctcg
    18301 cgccggactg cgaggtcgga gccctgccgc ccgtcgggcc tctgtatggc ctgtcgaccg
    18361 tggcagatca ccgcctgcgc gaggaccccg agatcagctt caacgccggc agccatcggt
    18421 tcgccgtgcg agtggagcgg gcggcctggg agcgagcctg ctcggtgatc tacgccaacc
    18481 tcgcggccga ccgggacgcc ggtccggcgt gggcccgatg acgcgctccg gcgccgcggc
    18541 tccggcccac gacccggccg acgccacggc aagtctcgtc tgggtcgacg cccggcaggc
    18601 gatcgtcctc cgctgggagc ggggcgcaac gaccgtcact cgccgggtat cggacgtgcc
    18661 gggccaccat cactcgaccg gccacgttcg tcacgatccg tccccgggca gcggcatcgc
    18721 cggtccgccg cgtgcggcag gtgagacgca tcgtctcgag cacctcgccc ggttcatcga
    18781 ggacgtggcc agcgccctgc cgaccgacgg cgacgtgatg gtgatcggcc ccggcacggt
    18841 ccacgagcga ctcgccgacc gactgcacgt cctggacgtc cggcacggcg gccatcgatc
    18901 ggtcgagcgc gaggcatcgg agccgttgac cgagcggcag ctgatcgccc gcctccgcac
    18961 cctggccggc tccccaaagc cgcgtcggat ccacgcgaat ccggcgcggg tgacggtccc
    19021 ggcgaggcgg gcctccggtt cgcccgcgag acgacccggg cgagggttcg acccaggcat
    19081 ccgccgccgg ccggaagccg ggttcgatga agaaccgttc ggcgacgacg agccgaccga
    19141 ggatctcgag ggcgccgggc tcgtcgacgc cgatcgagtc gtcaccgcgg gcggtgcgcc
    19201 gggcggcgcc gtcgtcgtca gcggggcgga tcccgggagt cctcgctcga cctgatcggg
    19261 actctggaca gccgggaaac agggtggccc gccggcatcc accggcgggc cattcaggat
    19321 gcggtcgagc gagaagcggc tgatgccatc atcgaccgcc gggtgtcgct gcaccgactg
    19381 gaacggggtt cgacggctcg ggcacgcctg cgggtcggag cgtggccgca atctccaccg
    19441 cccaggcgtc gatcgccggc cagtcgcggt agtcgccgaa cggcgcccgg accgccttca
    19501 cgatgagctt ctcgccgaac ccgagatcgc cgctcgcaag gcggccgggg aagacccggt
    19561 ggccacgcgg cccgatgagc tccttcaggg cggcgacccc ggccggttcc ccggccggaa
    19621 cgggcggatc gcccatcggc ccgctcgaga agagccagac cggacgctcg cggagctgca
    19681 tcgcgcagcg tgcgatcaag tcacgggcgg gggcaagcca gttgccagcg tacacaccgc
    19741 tgccgatgat caccgcttcg tacggcgtca ccgcgtcgat ctcctccgcg ggcacgacgt
    19801 ccgtggggat gcccgacgcg gcgagggcgc ggccgatggc ctgcccgatc tcgagcgtgg
    19861 cctgatgcct tgacgcaacc gtgacgagaa ccgtcatcga gggctccttc ctggaatggg
    19921 tggtcagcgg gccatggggg cggtggtcgg gcggaacagc gtcgacgtgg acacgccgcg
    19981 gacctgctct ccggccggaa cgacgagatc tcccccgacg gcaacgagga cgcgtccggc
    20041 gtgggccagg acctccccgg cggccaggac cgggaccgcc cacgtcagcg cgacgaatcc
    20101 ggccagcacc gcgcccagca accgtttcat gagatctcct tccgagcgcc cgttcggacg
    20161 cgcggacgcg cctggcgtcg aacggacgga tcgatcctga tcgtcgtgcc cgtgtggccc
    20221 gggcgagaag ggctggacga cccgtgggcc gtggccgaac ggcacttcct gccgccgggc
    20281 gcagagcctt gactggatcc acacgatgcc ggtgcgtggc cacgcaccga cccacccgcg
    20341 ggagcgacca cgatgctgat cattcgaaac gtcatgagcc aaccggccct gacggtccac
    20401 ccgacgactc cgttgaagga cgtcgcccgg ctcctcgtcg agcacaggat cagcggattg
    20461 ccggtcgtcg acgaaggcgg ccgcgtcctc ggcgtcgtct cggaggcgga cctcctgctc
    20521 aaggagcagg ggcccgaaca cgtcagccat cgccatttcg cccgccttct gggagagtcg
    20581 accgagactc gggcccagct cgcgaagctc gacgccacca cggccggcga cgcgatgtcg
    20641 gcaccggcga tcacgatcac cggcgaccgt ccgatcgctg aggccgcggc gctcatgacc
    20701 agccgcggca tcaaccgcct gcccgtcgtc gacgacggca ccctggtcgg cgtcgtcagc
    20761 cgaggggacc tgatcagcgc ctacgtccga tccgacgaag acctcggcga cgtcgtgcgc
    20821 aacgaggtcc tccatcggac gatgtggctc gacccgggcc tgttcgaggt cgtggttcag
    20881 gacgggatcg tccggatcgg cggctccgtc gggcgccgtt cgacggccga ggtcatcgag
    20941 cgcatgacgg ccatgatccc gggcgtcatc gatgtcatca gcgaactgac ctgggagacc
    21001 gacgatcgcg acctcgtcgc accggaacgc gacctggtct cggcctacac actcggccgc
    21061 tgaccggcca ccccggccgt ctcaggcggc gcgcatttcg gcgagcaggc ttcgtccttc
    21121 ctggaggtcg cccgctgacg cccgcgccag gcggtgttcc tcggccatga cgagcgacca
    21181 gaggtgggac tgcgtctcac gcagcagcgg ctcgccgcga tagcggtcgg cgagatcctc
    21241 gatgaacgcg ctgtagcgag cctctggtcg gagcgcggcc agccgggcct cgaagcccgg
    21301 cacatagtcg acggccacct ttgcccgacc gaagaaggcg ccgacgttct cggccgcctc
    21361 gtcccagccc tgcgaatggc ctccgcgccg gaggagcgga acggtcgagg tgaagacggc
    21421 gatggcgacg ccgtcgacga gcgcggccga cagccggcca ccgaaggtca ggtattcgtt
    21481 gccgacatgg agccggtcga cgatcctgat ctcgccccac gcgaacggcc gatcggcgat
    21541 cggcagcggg gtcgtccggg tgagctgttc gcgacgaccg acgccatcgc gagtgatcca
    21601 gtaatcgacg gcctccggat cgtagtgggt caatgccggc cgatcacgga gtgccacgag
    21661 caggtaggcc gatccggcct ggtcgggcag gtccgagtgg gccaggaacc cgagctgggt
    21721 gagcgtccgg aacgcatcgg cgggcgtcgg agcgtcgact tgccgagggt cgacggcgat
    21781 tgtccggtcg gccatggaag acctcgatcc gcgactgcca gccgaggacg gctgccggat
    21841 cgaatcctgc gcgttcgatc ggcggtcgcg aacgtgccga tcctcacgtt cggcggggcc
    21901 ggaccgcccc cggattccgc gacgggccgg acggccccgc cgttccgtgc cctccggctc
    21961 gtgaacggcg cccgggcgac ctcgacgatc gatccagcat tggaggtgct ggacatgggc
    22021 gggaaccgtt tcacacgatc ccctcgcccg cgaaccgaac acctgatcgc gctgaccgtc
    22081 gaggtcggcc aggtcgtctg tcccgcgcgg ggcatcaccg acatcgaaca ctgtttcacg
    22141 tgcagcgcct atcgcggtct gcaggacggc ccggccgagc gcctcgtctg cgcgcctgtg
    22201 accaggtcgg agctggcgta cgtacccctc ggattcgtcc cacgctaggg tgcccgagga
    22261 ggcgacggcg gggccgccgt cggtccgttg gacgcctgag gcgaaccgtg ctggacggcc
    22321 cctcccgggt ggacgaacgg ctctgcccgc ccggcggcga ccggccctag cctcggatca
    22381 tggacagcac ggccgtcgct gacgcgctcc gcggatcatc cctcgaactc ggcctcggcc
    22441 cgggatctcg gtgactggct ggacggtcct ggtctgcggc gaccggagtc gcggtgacga
    22501 cggcgcggcc atcgcggctg tcgaacgcct gccgcgccgc ctgcgagcgg agatccggat
    22561 ccgggcgcgc agccagctgg agcccgacga gctcgtctcc gttctcctgt ccggtccctg
    22621 cctcatcctt gacacggtcc acggcgtgcc gccgggtacg gtcgtcgagg tttcgctggc
    22681 gaagctgctc gccggcgacg gcccgacacc cgcttccacc catgcgctcc cgatgccgat
    22741 cgtcgtcggg ctcgcccggg ccctcggcgc gcccctcgag ac
//
LOCUS       DATAIW010000178         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_8716, whole genome shotgun sequence.
ACCESSION   DATAIW010000178 DATAIW010000000
VERSION     DATAIW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8716"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1279
                     /locus_tag="VHM48_05815"
     CDS             407..1279
                     /locus_tag="VHM48_05815"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2754958.1"
                     /translation="MTEPDLRTNPDALPDAEPPRIGRHPVNGTILYAEVRGSGPAVLL
                     IHAGGEDAEQWRPVAERLTGFTVVTYDRRGTLRSGRDAWPGGGSAQHADDAAGLLATL
                     GVDDVLVFGASSGGIPALQLALRHPELIRRALAYEPGYFRQVPGGEEFQRPANKAVAD
                     HLAAHPDDWVGAWAACRRAVARATNAGPRGFFTPPPGKEWYAEREDANAEALVVDDVP
                     ILTRELVDEKELATAPVDIRFSFGTASLPIFREIATHLASVRNATPDAIDGVGHSMPY
                     LPDAAAAYIRTGAE"
     gene            1639..>2119
                     /locus_tag="VHM48_05820"
     CDS             1639..>2119
                     /locus_tag="VHM48_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754959.1"
                     /translation="MLEAVFWGLVAASTLIVGATLAIALPWRRRMIGLGAGFGAGALI
                     SAVTLDLTRSAFEAADVPVVVVGLGAGALAFSAGNWALHRGGAVRHRKRSAGQQAGAD
                     PLGIVLGTVLDGIPESVVIGISLLTGEGVGLAFLAAVAISNLPEAISATTGLRRTGWS
                     "
BASE COUNT          292 a          759 c          738 g          330 t
ORIGIN      
        1 agcagcgcga gctcgagtca cccgctcgat cacgaggctt cccaccgaaa gggaggccga
       61 cgtcttccgc gacgggctag cgtggccggg atcggtcatc gagtcattcg agtacgagta
      121 tctcgacgag tgacccaccg attgctcccc ggctcacccg acgagccgcg cggacgctga
      181 tgcctgacga cgttccgcgc gaggctgcct ggttcgcctt ccatgacgcc ctcccggcct
      241 ggcccccgct cgggtcccgc cgtcgggatc agggcccagg acgtcgtgtc tccttcgccg
      301 acacgatgaa cccatggtga tcgcgagcca ggttctccgc acctctgacc ctcctcgttc
      361 ttgccctcac gatgacctgc tgcgctacgg cgaatacact gcctgagtga ccgagccaga
      421 ccttcggacg aacccggacg cgctgcccga cgccgagcca ccgcggatcg gtcgccatcc
      481 cgtcaacggc acgatcctgt acgcggaggt ccgcggctcc ggtccggccg tgctcctgat
      541 ccacgcgggt ggtgaggacg ccgagcagtg gcgtccggtc gctgagcgcc tgaccggctt
      601 caccgtcgtg acctacgacc gccgcgggac cctgcgcagc ggtcgagacg cctggccggg
      661 gggtggctcg gcacagcacg ccgacgacgc ggctgggctc ctcgccacac tcggcgtcga
      721 cgatgtcctc gtgttcggtg ccagttcggg cggaatcccc gcgctgcagc tggcgctccg
      781 ccacccggag ctgatccggc gcgcgctcgc ctatgagccg gggtacttcc ggcaagtccc
      841 tggcggcgag gagttccaac ggccagcgaa caaggcggtg gccgatcacc tggcagccca
      901 tccagacgac tgggtcggag cgtgggcagc ctgtcgccga gccgttgccc gagcgacgaa
      961 cgccggccca cgcggcttct tcactccgcc gcccgggaag gagtggtacg cggagcgcga
     1021 ggacgccaac gccgaggcgc tcgtggtcga tgacgtcccc atcctgaccc gcgaactcgt
     1081 cgacgagaag gaacttgcga cggctccggt cgatattcgg ttctccttcg gtacagcctc
     1141 tctgccgatc ttccgagaga tcgcgaccca cctcgcctct gttcgcaacg ctacccctga
     1201 cgccatcgac ggtgtcggtc attcgatgcc atacctcccc gacgcggctg ctgcatacat
     1261 ccgcaccgga gcggagtgac atagcgcggc gttcgcaccg cgacgtcgac gacagcgccg
     1321 gagtcgcgac acgatgacct ggtcgtgctg cttctcttca ggttggtgct tggcgatgcg
     1381 tcgactcacg cgtggcgccc gtcacccgcc gacggtgctg ccgcgatcgc cgggcggcgc
     1441 cctccgcgag ccgcggcccg acgacccgag aacgacgccg ttgaccagct cacgcacgag
     1501 gcgcgacggc gaagacggcg gaggacggcc cagccgacga agcgctcgcg tatcgcgcga
     1561 tggtagccgc gccagtggca tcctgacgcc gagcgtcggg cagccctcgg cgcgacgacg
     1621 cctgccggag gattctcggt gctcgaggcg gtgttctggg gcctggtggc ggcttcgacg
     1681 ctgattgtcg gtgccaccct ggcgatcgcc ctgccgtggc ggcggcggat gatcggcctt
     1741 ggcgccgggt tcggggcggg cgccctgatc tcggcggtga ccctggacct gacgaggtcg
     1801 gcgttcgagg cggccgacgt ccccgtggtc gtggtcgggc tgggcgccgg cgcgctcgcc
     1861 ttctcggccg gcaactgggc gctccaccgc gggggagcgg tgcgccaccg caagcggtcg
     1921 gcaggccagc aggcgggcgc cgacccgctc gggatcgtgc tcgggacggt cctcgacggg
     1981 atcccggagt cggtagtcat cgggatttcg ctcctgacgg gggagggcgt cgggctggcg
     2041 ttcctggcgg ccgtcgcgat ctccaacctg ccggaggcga tctcggcgac gaccggcctg
     2101 cgccggaccg ggtggtcga
//
LOCUS       DATAIW010000179         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212463, whole genome shotgun sequence.
ACCESSION   DATAIW010000179 DATAIW010000000
VERSION     DATAIW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212463"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1080
                     /locus_tag="VHM48_05825"
     CDS             136..1080
                     /locus_tag="VHM48_05825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2754960.1"
                     /translation="MRGLAIVLVLIEHTATSPAGYLLRIAPAMAGVTVFFVLSGYLIT
                     GLLARERPIGLRNFYARRAIRLGPALLVLITAVVVLSILGRNPFPWQPGVISSLFYVS
                     NWADVAGIPTGLLGHTWSLSIEEQFYLVWPVIMLTVPRRWLLPLSVGLAVSGCLLYFL
                     NVGPVYYSTLTNGGAILAGCAIALAERQLPRIAGLLGIALIIVAAAIVSQALAVVGAV
                     LLLLGPVDALIPIAPVGRRAYSLYLWSWPFAYLVGGAAALPLTIIAAELSYRLVEQPV
                     RHRFHGRFAPHRGRMTVDLPRSAGVVPPRPDLEEPAPV"
     gene            complement(1077..2279)
                     /locus_tag="VHM48_05830"
     CDS             complement(1077..2279)
                     /locus_tag="VHM48_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2754961.1"
                     /translation="MGAPPIWDPCLAPLEHFPMPGPKRVRVVEILATGTNGGAQEHVF
                     GLMTRMDTTHYEASVVSLSAGSAVRKLERHGFDVTVIDQPDDADAVRALTTLLARVQP
                     DIIHTHMYRADLVGTRAAMALGESGRRRPYIVSTVHSSRVRSHEDRELLRRLTPEMDQ
                     LIAVSKSIERKIAAEHRTGAPVRLIYNGVDLQRYDHTDPCCTLRDEYGMEPGAPIVGV
                     VARLEPEKGHQTLIEAWPQVLREVPAAYLLIVGEGSRRDFLEQWAAAHKVAHRVVFAG
                     RRDDIPAVTAAIDVAVLPSWREAQGLSILEAMALSRPVVASDVGGIPEMIEDGVTGLL
                     VEHDNPVALAAAIVRLLTDHAYADTLARAGHDLVHDRFCIELMVKAVEQIYDEGARAV
                     RLSESAAS"
     gene            complement(2279..3538)
                     /locus_tag="VHM48_05835"
     CDS             complement(2279..3538)
                     /locus_tag="VHM48_05835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2754962.1"
                     /translation="MTTHAPNAEPACPGSTDAGTLERNNDAAGSAAALHVERRPFGDI
                     PRATWDALAARNPWATPFSSWAFQRAWWDAYGASAHDQTLIVTAMSGAAGGAEAGDPV
                     AIVPLMHRHEVEPSDAETASKLRHGDAAHLTPVAPTAKAVFFGASYHADYATILAAPA
                     DLPAVATALVDHLGAADPSDPEHPAPWDAIDLRRLRCGDPAADALAAAFGRREISDGW
                     TLNLEREDVCPVVTFPDGLGFEDFLSTLDKKARHEIRRKIRRAEAAGELDLALAPDAL
                     ADLDEFIDLHQRRWGADGLFPPTPGGDASRVFIRRWFEESGPDGPVQLLFLTVGGRRI
                     AAGIHVVAGDTVMFYNAGIDPDARDLSAGVILAARCVQFAVEHGQRRFDFLRGNEPYK
                     YEWGATDEPIQRLLVRRDGAGSDEALG"
BASE COUNT          533 a         1307 c         1414 g          610 t
ORIGIN      
        1 gctgacgggt ggtcgtcgac tgggtcactc gcgctcatca tcgccggatc tccacggagc
       61 acccgcttgg gcctgtccca tctcctcggt cgcacgcagc cgcgatggac aacgtaccga
      121 cccgaccttg acgggatgcg cggcctcgcg atcgtcctgg tcctgatcga acacaccgca
      181 acctcgccgg cgggctacct gctccggatc gcaccggcga tggcgggagt caccgtgttc
      241 ttcgtcctgt cgggctacct catcaccggt ctcctggccc gtgagcgccc gatcgggctg
      301 cggaacttct acgcgcgtcg cgcgatccgg ctcggtccgg cgctcctcgt cctcatcacc
      361 gcggtcgtcg tcctgtcgat cctcgggcgc aacccgttcc cgtggcagcc gggcgtgatc
      421 agctcgctct tctatgtgtc caactgggcc gacgtcgccg gcatcccgac gggcctgctt
      481 ggccacacct ggtcgctctc gatcgaggag cagttctacc tggtgtggcc cgtgatcatg
      541 ctcaccgtgc cccgccgttg gctactgcca ctgtccgtcg gtctggcggt ctccgggtgc
      601 cttctctact tcctgaacgt cggaccggtg tactactcga ctctgaccaa cggcggcgcc
      661 atcctcgccg gctgtgccat cgcactcgct gaacgacagc tgccgaggat cgccggcctg
      721 ctcgggatcg cgctgatcat tgtcgctgcg gccatcgttt cgcaggcgct ggcggtcgta
      781 ggggcggtcc tcttgctact cggccctgtc gacgccctga tcccaatcgc accggtcggg
      841 cgccgagcct actccctgta cctgtggagc tggccgttcg cctacctggt gggtggcgcg
      901 gcggcgctgc cgctgaccat catcgccgcg gagctgtcgt acaggctcgt cgaacagccg
      961 gtgcgacaca ggttccacgg gcgcttcgcg ccgcacaggg gcaggatgac cgtggatctc
     1021 ccgcggtcgg cgggtgtcgt tccgccgcgg ccggatctcg aggagcccgc accggtctag
     1081 cttgccgccg actccgacag gcggaccgcc cgcgcgccct cgtcgtagat ctgctcgacc
     1141 gccttgacca tcagctcgat gcagaagcgg tcgtggacga ggtcgtggcc ggcccgggcg
     1201 agcgtgtcgg cgtaggcgtg gtcggtgagg aggcggacga tcgcggcggc cagcgccacc
     1261 gggttgtcgt gctcgacgag gagcccggtg acgccgtcct cgatcatctc cgggatcccg
     1321 ccgacatcgg acgcgacgac gggccgcgag agcgccatcg cctcgaggat cgagagcccc
     1381 tgggcctcgc gccaggacgg caggacggcg acgtcgatgg cggcggtgac ggccgggatg
     1441 tcgtcgcggc ggccggcgaa gacgacgcga tgggcgacct tgtgggccgc ggcccactgc
     1501 tcgaggaagt cgcgccgcga gccctcgccg acgatcagca ggtaggcggc ggggacctcg
     1561 cgcaggacct gcggccaggc ctcgatcaac gtctggtggc ccttctccgg ctcgagcctc
     1621 gccacgactc cgacgatcgg agcgcccggc tccatcccgt actcgtcgcg gagcgtgcag
     1681 cacgggtcgg tgtggtcgta gcgctggagg tcgacgccgt tgtagatcag gcggacgggc
     1741 gctccggtcc ggtgctcggc ggcgatcttg cgctcgatcg atttcgagac cgcgatcagc
     1801 tggtccatct ccggggtcag gcggcggagg agctcgcgat cctcgtggga gcggacgcgc
     1861 gacgaatgga ccgtcgagac gatgtacggc cggcgacgcc cactctcgcc gagggccatc
     1921 gccgccctcg tgccgacgag gtccgcccgg tacatgtggg tgtggatgat gtccggctgg
     1981 acgcgggcga gaagagtggt cagggcgcgg acggcgtcgg catcatcggg ctggtcgatc
     2041 acggtgacgt cgaagccgtg ccgctcgagc ttgcggacgg cgctgccggc cgacagcgac
     2101 acgaccgacg cctcgtagtg ggtcgtgtcc atccgcgtca tcagcccgaa cacgtgctcc
     2161 tgggcgccgc cgttcgtgcc ggtcgcgagg atctcgacga cccgcacgcg cttcgggccg
     2221 ggcatcggga aatgctcgag cggggcgaga cacggatccc agatcggcgg ggcgcccatc
     2281 agccgagagc ctcgtcggac ccggctccgt cccgccggac gagcaggcgc tggatcggtt
     2341 cgtcggttgc gccccactcg tacttgtacg gctcgttgcc gcggaggaag tcgaatcggc
     2401 gctggccgtg ctcgacggcg aactggacgc agcgagcggc gaggatgacg cccgccgaaa
     2461 ggtcacgggc atcggggtcg atgccggcgt tgtagaacat cacggtgtcg cccgcgacga
     2521 cgtggatccc ggccgcgatc cgtcgcccgc cgacggtcag gaacaggagc tggacgggac
     2581 cgtccggacc ggactcctcg aaccagcggc ggatgaagac ccgcgatgcg tcaccgcccg
     2641 gcgtcggcgg gaacaggccg tcggcacccc agcggcgctg gtggaggtcg atgaactcgt
     2701 cgaggtcagc gagggcgtcg ggcgcgagcg cgaggtcgag ctcgccggcc gcctcggcgc
     2761 gacggatctt ccggcggatc tcgtgccggg ccttcttgtc gagggtcgag aggaagtcct
     2821 cgaagccgag gccgtcgggg aacgtgacga ccgggcacac gtcctcacgc tcgaggttga
     2881 gcgtccagcc gtcgctgatc tcgcgccggc cgaacgcggc ggcaagggcg tcagcggccg
     2941 ggtcgccgca gcgcaggcgg cggaggtcga tcgcgtccca cggcgccggg tgctcgggat
     3001 cggacgggtc ggcggcgccg agatggtcga cgagggccgt cgcgaccgcc ggcaggtcgg
     3061 cgggggcggc caggatcgtc gcgtagtcgg cgtggtagga ggcgccgaag aagacggcct
     3121 tggcggtcgg cgcgaccggc gtgaggtggg cggcatcgcc gtggcggagc ttcgaggcgg
     3181 tctcggcgtc gctcggctcg acctcgtggc gatgcatcag gggcacgatc gcgaccggat
     3241 cgccggcctc cgcgccgccg gccgcgccgg acatcgcggt gacgatcagc gtctggtcat
     3301 gggcgctcgc gccgtacgcg tcccaccacg cccgctggaa cgcccaggag gaaaagggcg
     3361 tcgcccacgg attgcgagcc gcgagcgcgt cccacgtggc ccgcgggatg tcgccgaacg
     3421 ggcggcgctc gacgtgaagg gcggccgcag acccggccgc atcgttgttg cgttcgaggg
     3481 tcccggcgtc agtcgagccc ggacaggcag gctccgcgtt cggagcgtgg gtggtcacgg
     3541 ccggaggata tcaccgggtc ccccgaatcg attgcgggcg caacggagcg tgggccggcg
     3601 ctgccggact tgttcatctg atgcacggtt ccgacatccg cgttcactag atgaacctac
     3661 gcgacctgat gggcagtggc gggctccgca gacagggttc cgcggcgagt ccgaaactgg
     3721 aaacgggcga atcggttcat ctgatgacgc aattcgtgcg ggtttcggcg tctcgacggc
     3781 gcgtcgtgtc cgatcggttc atttgatgca cggcctcggc cctaccagag cgggcgcagg
     3841 aatcgcagga tgtccgtgaa gtcg
//
LOCUS       DATAIW010000180         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212471, whole genome shotgun sequence.
ACCESSION   DATAIW010000180 DATAIW010000000
VERSION     DATAIW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212471"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHM48_05840"
     CDS             complement(<1..315)
                     /locus_tag="VHM48_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754963.1"
                     /translation="MIEIAGLVHVYPDGTRALDGVDLAIGPGETLAIVGQNGSGKSTL
                     VRHLNGLLRPTEGTVRIDGRDIAGQRVARLAATVGLVFQDPDRQIFAGKVRTEVAFGP
                     RNL"
     gene            complement(312..1112)
                     /locus_tag="VHM48_05845"
     CDS             complement(312..1112)
                     /locus_tag="VHM48_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754964.1"
                     /translation="MLELRGVSYRYAGYAMAVVHDIDLALGDGEIVGLVGPNEAGKST
                     ICLLACGLAPASIGGGLTGSLVIDGQSMTGRPVHELAGRVVLGFQNPATQRSGVAATV
                     FEEVALGPMNLGLSVAATLARTRAALERLRIEHLAERDPARLSGGQGQLVAIASLLAM
                     NPRHIVLDEPTAQLDPEGTRLVGEALRALAATGTALLVAEHKTDVLDGLCGRVAVVVD
                     GRIVLDGPASRVFGDERLEGWGVEPPSRIRLSRALAGTGVDVTAALAR"
     gene            complement(1119..1925)
                     /locus_tag="VHM48_05850"
     CDS             complement(1119..1925)
                     /locus_tag="VHM48_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX2754965.1"
                     /translation="MSEADAAYLASLAPAAPSAYHRLNPLTKAVAAIVCSLGAFVLGG
                     YVGPLVILLGGIIPSAISARLLGRLLRISILLSLPIAISIVLVSVFTRAGTTVLFAIG
                     PFDATLEGVDFAGQVLVRLLVLSTAVGLFGLTTDPRALVFDLERRGMSPKLGFAAVAT
                     VEAVPSLIERAGVIQAAQRARGLDTEGRLIARFRGIMPIVGPVLLSSLTEVEERSLAL
                     EVRAFGRPGRRNLLWWPGDTSAERVVRWVLVVGFLGLVAARAAGLLPRIP"
     gene            complement(1934..3007)
                     /locus_tag="VHM48_05855"
     CDS             complement(1934..3007)
                     /locus_tag="VHM48_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754966.1"
                     /translation="MAGTWVRIGIGVLAGIAVAAIGLLALGTVAASTDPVTGGNTQVA
                     NGIMLVLAIAILVAVVVYAVVEYAQTGRIRSITSQFDTRTIVLIPIAIAINIILGQTV
                     SAALKVPVYLDSIGTILVGALAGPIPGALTGLLSNTIWTFILGGTPLGSPYAWPFAIV
                     AAEIGLLAGIFGRLGFFRSRPNTPTPKLLAGAAVALVILAALGYWGVLPAFTKGVTFF
                     GDLTTVNSFFVVVAWLIAIGLVGAGIGLVALLLVRRDLGVAFIVVCGAICGVVSALIS
                     APISAYFFGGVTGSGTDFLVAAFQQAGSTLSSAVLQQGLISDPIDKTITYIVVFTLLQ
                     VLSRRFTARFPQGEQAVGLIGSD"
     gene            complement(3259..>4138)
                     /locus_tag="VHM48_05860"
     CDS             complement(3259..>4138)
                     /locus_tag="VHM48_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2754967.1"
                     /translation="ETHGPRGIGYAVLPPPSRPLSARFGPDLIVEEARRRPGELTLVT
                     LGPMTNLAIAVLVEPDLPMLLRRWVMMGGSYRSPGNTAPTTEWNVSVDPDAAKLCIAS
                     FGRPDVTDARRVAGLPPLPLALGLDVTERAKLLPRHLAALAARAGAPTAHSRDAAIGA
                     GIDASGAGIDASGADGSGVGADASIPANPVIRFIDDALRFYMEFHSRYDGFYGAFVHD
                     PFAVAAALDPSLVRTEALSVDVELDGTLTTGETVTDWRRAWGRPPTLDVAVEADAAAF
                     FERFIERVGGLAERVG"
BASE COUNT          682 a         1480 c         1471 g          505 t
ORIGIN      
        1 aaggttccgc ggcccgaacg ccacctccgt ccgcaccttg ccggcgaaga tctggcggtc
       61 gggatcctgg aagaccaggc caaccgtggc ggcgaggcgg gcgacgcgct ggccggcgat
      121 gtcccgcccg tcaatccgga cagtcccctc ggtcggacgc aggaggccgt tgaggtggcg
      181 aacaagcgtc gacttcccgc tcccgttctg gccgacgatc gccagggtct caccgggtcc
      241 aatcgcgaga tcgacaccgt cgagtgcccg ggttccgtcg gggtagacgt ggacgaggcc
      301 ggcgatctcg atcatcgggc aagcgccgca gtgacgtcga cgccagtccc cgcgagcgcc
      361 cgggaaagcc ggattcgcga cggtggctcc acgccccagc cctccagccg ctcgtcaccg
      421 aaaaccctcg aggcgggccc gtcgaggacg atccgcccat ccacgaccac ggccacccgc
      481 ccgcacaggc cgtcgagcac gtccgtcttg tgctcggcga cgaggagcgc cgtcccggtc
      541 gcagcgaggg cgcgaagcgc ttcgccgacg agccgcgtcc cctccgggtc cagctgggcg
      601 gtgggctcgt cgagaacaat gtgacgtggg ttcatcgcca gaaggctcgc gatcgcgacc
      661 agctggccct gcccgccgga gagccgggcc ggatcgcgct ccgcgaggtg ctcgatccgc
      721 aggcgttcga gtgcggcgcg cgtgcgcgcg agcgtcgcgg cgaccgacaa gccgaggttc
      781 atcgggccca gcgccacctc ttcgaagacc gtcgcggcaa ccccggatcg ctgggtggcc
      841 gggttctgga agcccagcac gacccgcccg gccagctcgt ggaccgggcg tcccgtcatt
      901 gactgcccgt cgatgacgag cgagccggta aggccgcccc cgatcgacgc cggcgcgagc
      961 ccgcacgcga ggaggcagat ggtggacttg ccagcctcgt tcggcccgac cagcccgacg
     1021 atctcgccgt cgccgagcgc gaggtcgatg tcgtggacga ctgccatcgc atacccggca
     1081 tagcggtagg agacgccgcg gagctccagc atgggtgctc agggaatccg cgggaggagg
     1141 cccgccgcgc gagcagcaac aaggccgagg aatcccacta cgagcaccca tcggacgacc
     1201 cgttccgcgc tcgtgtcgcc cggccaccag agcagattcc gccgcccggg ccggccgaac
     1261 gcgcgcacct ccagcgccag gctccgctcc tcgacctccg tgagagacga gagcagcacg
     1321 ggaccgacga tgggcatgat cccgcggaag cgagcgatca gccgtccctc cgtgtcgagt
     1381 ccccgggccc gctgggcggc ctggatgacg cccgcccgct cgatcagcga ggggaccgcc
     1441 tcgaccgtcg cgaccgccgc gaacccgagc ttcggcgaca tgccgcgtcg ctccaggtcg
     1501 aagacgagcg cccgcggatc cgtcgtcagc ccgaacagcc cgaccgccgt cgaaagcacg
     1561 aggagccgca ccaacacctg cccggcgaaa tcgactccct cgagagtcgc gtcgaacggg
     1621 ccgatggcga acaggacggt cgtgcccgct cgggtgaaca cgctgacgag gacgatcgag
     1681 atcgcgatcg ggagggacag gaggatcgag atccggagca ggcgacccag caggcgggcc
     1741 gagatcgcgg acgggatgat gccgccgagc aggatcacga ggggcccgac gtagccgccc
     1801 agcacgaacg cgccgagcga gcagacgatg gctgcgaccg ccttggtgag cgggttcagc
     1861 cggtgatagg cgctgggcgc cgcaggcgca aggctcgcga ggtatgcggc gtcggcctcc
     1921 gacacgtcgt cgatcagtcg ctcccaatca ggccgaccgc ctgctcaccc tgggggaacc
     1981 gggcggtgaa ccgacgcgag agcacttgga ggagcgtgaa gacgacgatg taggtgatcg
     2041 tcttgtcgat cggatcggag atcaggccct gctgaagcac cgccgaggag agggtcgacc
     2101 ccgcctgctg gaacgcagcg acgaggaagt cggtcccgga gccggtgacg ccaccgaaga
     2161 agtaggcgga gatcggtgcc gagatgagcg cggagaccac cccgcatatt gctccgcaga
     2221 ccacgatgaa ggctacgccc aggtcgcgcc ggaccagcag aagggcgacg agcccgatgc
     2281 cggcgccgac cagcccgatc gcgatcagcc aggccacgac cacgaagaac gaattgaccg
     2341 tggtgagatc gccgaagaac gtgacgccct tggtgaaggc gggcagcacg ccccagtagc
     2401 ccagcgccgc caggatgacc agggccaccg ccgcccccgc caggagcttc ggcgtgggcg
     2461 tgttgggtcg cgagcggaag aagccgaggc ggccgaagat ccccgcgagc agcccgatct
     2521 ccgcggccac gatcgcgaac ggccacgcat acgggctgcc cagtggcgtg ccgccgagga
     2581 tgaacgtcca gatcgtgttc gacaggaggc cggtgagcgc tcctgggatc gggccggcca
     2641 gcgcgccaac gaggatcgtg ccgatcgaat ccaggtacac gggcaccttc aaggcggccg
     2701 agaccgtctg gcccaggatg atgttgatcg cgatcgcgat cgggatcagg acgatggttc
     2761 gggtatcgaa ctgcgacgtg atcgaccgga tccggcccgt ctgggcgtac tccacgacgg
     2821 cgtacacgac gaccgcgacg aggatcgcga tcgcgagcac gagcatgatg ccgttggcga
     2881 cctgggtgtt gccgcccgtc actgggtcgg tcgaggccgc gacggtgccc aacgccagca
     2941 ggccgatcgc ggcgaccgcg atcccggcca gcaccccgat cccgatgcgg acccatgttc
     3001 cggccactga tgtgcctccc gtcctcggtc gatcgctctc gaccctcgcg cgaaccgcca
     3061 acttcgtcgg cctccgggtt ccctccgccc acggcccggc gcgccccgaa gtctcccacg
     3121 cgggctggtc tccgcgccag tcttcgccgg gcattgggta cttggtccgc ggcccgacgg
     3181 ccccgctccg gtcgcggcgg tcgacgtgca ggtcgacgtg gggatcggcc cccggcggcg
     3241 cgtccggccg tccggtgctc agccgacgcg ctccgcgagc cccccgacgc gctcgatgaa
     3301 ccgctcgaag aaggcggcgg cgtcggcctc gaccgccacg tccagggtgg gtggccggcc
     3361 ccaggcgcgg cgccagtccg tgaccgtctc gccggtcgtg agcgtgccat ccagctccac
     3421 gtccacggag agtgcctccg tccgcacgag cgacgggtcc agcgccgccg ccacggcaaa
     3481 ggggtcgtgg acgaacgcgc cgtagaagcc gtcgtagcgg gaatggaact ccatgtagaa
     3541 gcgaagcgca tcgtcgatga agcggatgac cgggttcgcg gggatggacg cgtcggcgcc
     3601 gacaccggac ccgtccgcgc cggacgcgtc gatgccggca ccggacgcgt cgatgccggc
     3661 accgatcgcc gcgtcccggg agtgcgcggt gggtgccccg gcccgggctg ccagcgccgc
     3721 gaggtggcgg ggcagaagct tcgcgcgctc cgtgacgtcg aggcccaggg ccagcgggag
     3781 cggcggcagg ccggcgaccc ggcgggcgtc ggtcacgtcc ggccgcccga acgacgcgat
     3841 gcagagcttc gcggcatccg ggtccacgct cacgttccac tcggtcgtcg gggcggtgtt
     3901 gcccggcgac cggtagctcc cgcccatcat cacccagcgc cgcagcagca tcggcagatc
     3961 cggctcgacg aggacggcga tcgcaaggtt cgtcataggc ccgagggtca cgagcgtcag
     4021 ctcgcccggg cgccggcgcg cctcctccac gatgaggtcc ggcccgaacc gcgccgagag
     4081 cggtcggctc ggcggtggca ggaccgcgta cccgatcccc cgcggaccgt gcgtctcg
//
LOCUS       DATAIW010000181         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_98640, whole genome shotgun sequence.
ACCESSION   DATAIW010000181 DATAIW010000000
VERSION     DATAIW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98640"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VHM48_05865"
     CDS             <1..1533
                     /locus_tag="VHM48_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2754968.1"
                     /translation="RHEGAAAFMAEAHGQLTGRPAAAIGTRAVGAANLAIGIHTARQD
                     SSPMFALVGQVERQVRGREAFQEIDQASTIGGLATHAVELTAVADVPQMVGDAVRAAL
                     VGRPGPAFLSIPEDILDEELPASAQLDTSRPAPIRPEPDDVRAVLQFLAAAERPVILA
                     GGGVLRARTSTDLIRLAELLRVPIVAGWRRADVIPNDHPLYLGMAGYGSPSVVRERLE
                     RADAVLVLGCRLSEITSFGYAIPAEGQPWMHVDVEPLDDRPGLPIAVRTVRADARAFL
                     RAAVSRLQEAVLDAASADARETVNVADRTAWEAATTPDAAAWTGPGVHPAHAVTAVRR
                     LIPDDAIITTDAGNFGLWVARHFRFRRPGTFLGPTSGAMGYALPAAIAAGLVHRDRVV
                     VAFAGDGGFAMTMAELETAVREKSKVIAIVFDNERYGTIRMHQDRRGGGAEGAIATDL
                     GPIDFAAIARACGARGIRVETDAAFEQAVRQALANDRPTVIQVALDRGWVAPDRPFAP
                     GG"
     gene            1526..1876
                     /locus_tag="VHM48_05870"
     CDS             1526..1876
                     /locus_tag="VHM48_05870"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX2754969.1"
                     /translation="MADPSPRLTYHLAPAAWWDAADPHAPLSAPSLESEGFIHCTDGS
                     AAMVETANRHYVADPGDFVVLTVDLARVTSPWRVEDPGRIYPHVFGPIDRSAIVAVVL
                     APRDADGGFLPFET"
     gene            1903..>2576
                     /locus_tag="VHM48_05875"
     CDS             1903..>2576
                     /locus_tag="VHM48_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754970.1"
                     /translation="MARATKVPIDPDDAPLKRLGGGRWQSRDERFTIEPQSGTWVVVD
                     GEQIDDLGMALVRGPFPSLTAAKEAIGRARSSVPAASPLAGRIERRRREPHAEPRPNA
                     DAIGLGKTSRASSMQGRHPRAGDEDGAETGSTPGRPRAASRDRKPVSHSGEEPPEPGW
                     LTDLEPGERGRARRLIARLRADDVPDAEGLVRRDLVGDVPALAAQAIAHRLGDLGDDA
                     SPRDVA"
BASE COUNT          333 a          942 c          935 g          366 t
ORIGIN      
        1 cgtcacgagg gcgccgccgc cttcatggcc gaggcccacg gtcagctgac cgggcggccg
       61 gcagcggcga tcgggacccg ggcggtgggt gctgccaacc tcgcgatcgg gatccatacg
      121 gcgcgccagg attcctcacc gatgttcgcc ctcgttggcc aggtcgagcg tcaggtccgt
      181 ggtcgcgagg cgttccagga gatcgaccag gcatcgacga tcggcggcct cgccacccat
      241 gccgtcgagt tgacggccgt cgcggacgtg ccgcagatgg tcggcgacgc cgttcgagcc
      301 gccctcgtcg ggcgacccgg cccggcgttc ctctcgatcc cggaggacat cctcgacgag
      361 gagctcccgg cctcggcgca gctcgacacc tcgcggccgg cgccgatccg accggagccg
      421 gacgatgtcc gggcggtgct ccagttcctc gccgccgcgg agcgaccggt gatcctcgcg
      481 ggcgggggcg tcctccgggc acggacgtcg accgatctca tccgcctcgc cgagctccta
      541 cgggtgccga tcgtcgccgg gtggcgtcgc gcggacgtga tcccgaacga ccacccgctg
      601 tacctcggca tggcgggcta cggcagcccg tcggtcgttc gcgagcgcct cgagcgcgcc
      661 gacgccgtcc tcgtcctcgg ttgccggctc tcggagatca cctcgttcgg ctacgcgatc
      721 ccggccgagg gccagccctg gatgcacgtc gatgtcgagc cgctcgacga tcgaccaggt
      781 ctgccgatcg ccgtgcgcac ggtccgcgcg gacgctcggg cgttcctgcg agccgccgtc
      841 tcgcgtctcc aggaggcggt gctcgacgcc gcgtcggccg acgcgcgcga gaccgtcaac
      901 gttgccgatc ggaccgcttg ggaggcagca acgaccccgg acgcggcggc ctggaccggg
      961 ccgggcgtgc atcccgccca cgcggtcacc gcggtccgcc ggctgatccc cgacgacgcg
     1021 atcatcacca ccgatgccgg caatttcggc ctgtgggtcg ctcgccattt ccggttccgc
     1081 cggccgggga cgttcctcgg gccgacgtcc ggggcgatgg gctacgccct gccggcggcg
     1141 atcgccgcgg gcctcgtcca ccgcgatcgg gtcgtcgtcg ccttcgctgg cgacggcggg
     1201 ttcgcgatga ccatggcgga gctcgagacc gccgtgcgcg agaagtcgaa ggtgatcgcg
     1261 atcgtgttcg acaacgagcg ctacggcacg atccggatgc accaggatcg gcggggtggg
     1321 ggggccgagg gcgcgatcgc gacggacctc ggaccgatcg acttcgccgc gatcgctcgc
     1381 gcctgcggag cgcgcgggat ccgcgtcgag accgatgccg cgttcgagca ggccgtccgc
     1441 caggccctcg cgaacgaccg cccgaccgtg atccaggtcg cgctcgaccg gggctgggtc
     1501 gcgccggacc ggccgttcgc gcccggtggc tgacccgtcg ccgcggctga cctatcacct
     1561 cgctccggcg gcgtggtggg acgcggccga cccgcacgca ccgttgagcg cgccatcgct
     1621 cgagtccgaa gggtttatcc attgcacgga cggctccgcg gcgatggtcg aaaccgccaa
     1681 ccgccactac gtcgcggatc cgggcgactt cgtcgtcctg acggtcgatc tggcgcgagt
     1741 cacgagcccg tggcgggtcg aggaccccgg ccggatctat ccgcatgtct tcggcccgat
     1801 cgaccggtcc gcgatcgtcg ccgtcgtcct cgcaccgcgc gacgcggacg gcggcttcct
     1861 ccccttcgaa acctgacggg tccgggacgg tatcgtctcg tcatggctcg agcgaccaag
     1921 gtaccgatcg acccggacga cgcgcccctc aagcggctcg gtggcggacg ttggcagagt
     1981 cgggacgagc gcttcacgat cgagccccag tcggggacgt gggtcgtcgt cgacggcgag
     2041 cagatcgacg atctcggcat ggcgctcgtg cgtggcccgt tcccgagcct gaccgccgcc
     2101 aaggaggcaa tcgggagggc gcggtcgagc gtgcccgcag cctcgccgct ggcggggcgg
     2161 atcgagcggc gccgccggga accacacgcc gaaccgaggc cgaatgccga cgcgatcggg
     2221 ctgggcaaga cgtctcgcgc gtcgtcgatg cagggacgcc accctcgggc gggtgacgaa
     2281 gacggggctg agaccggttc gacgccgggc cggcctcgag ccgcgtctcg cgatcggaag
     2341 cctgtgtcgc attccgggga ggagccgcct gaacccggct ggctgaccga tctcgagccc
     2401 ggggaacgcg gccgcgcccg ccggttgatc gcccgcctcc gcgccgatga cgttcccgac
     2461 gccgagggcc tggtccggcg tgacctcgtc ggcgatgtgc cggcactggc tgcccaggcg
     2521 atcgcccacc ggctcggcga tctcggtgac gacgcgtcgc cgcgggacgt tgcgga
//
LOCUS       DATAIW010000182         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_8949, whole genome shotgun sequence.
ACCESSION   DATAIW010000182 DATAIW010000000
VERSION     DATAIW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8949"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHM48_05880"
     CDS             <1..232
                     /locus_tag="VHM48_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754971.1"
                     /translation="EKVLRSYRRVLIPEVNLGQLLMLIRSQFLIDAEGYDRVRGKPFT
                     IAEIVTQAERILGPMASRSSASDLGERANDHD"
     gene            225..1298
                     /locus_tag="VHM48_05885"
     CDS             225..1298
                     /locus_tag="VHM48_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX2754972.1"
                     /translation="MTEGANPMTDATQPALSAANAAVIQLTRKDFVSDQEVRWCPGCG
                     DYSILAQTQKQMPDFGVPKENIVFISGIGCSGRLPYYMNTYGFHTIHGRAPTLATGLK
                     AARPELMVWVITGDGDALSIGGNHVIHTMRRNVDITMVMFNNRIYGLTKGQASPTSEF
                     GKKTKSTPAGTIDTPIMPLTIALAAEATFVARSVDTHTEHLQQTLQRAGMHHGSTFVE
                     VLQNCNIFNDGAWRDFTDREVREDRMLFLEHAKPMIFGKDRDKGIRLRGLHPEVVTIG
                     ENGVTEADLLVHDEQAEEPYLGLMLSRMFWPEYPVPVGVLRAVARPTHDQLIDQQISA
                     AVAATGAGDLAEALASGETWLVE"
     gene            1312..1812
                     /locus_tag="VHM48_05890"
     CDS             1312..1812
                     /locus_tag="VHM48_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2754973.1"
                     /translation="MRCPTCGYENLLGSDVCDNCGSDLAGHDVPQSPSTFRGQLLGEH
                     LDALEIVAPETIEATADVNEAIRRMHDNAIDCVLVTEAGRLVGIFTDRDAVVKVAGLE
                     RRVRPIATLMTRDPVVLRHDDTIAVAINKMAFGGFRHIPIVEDGRPTGVVTARDVFRH
                     LVESLG"
     gene            1826..2242
                     /locus_tag="VHM48_05895"
     CDS             1826..2242
                     /locus_tag="VHM48_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754974.1"
                     /translation="MTMVESGASIRVVVLADDLIWSTRLVGHVRAAGFEAVPARSING
                     FLSGLADAGGAIVDLTSRAYDGLAAIAAARAAGVPVLAVGQHDDHVLRRDALAVGADR
                     VLAYRKLFEDGPATIARWLAASSSTPSRQPTETTTR"
     gene            2239..>3171
                     /locus_tag="VHM48_05900"
     CDS             2239..>3171
                     /locus_tag="VHM48_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2754975.1"
                     /translation="MTTATAAHPRIPAERYAERLQRLAALVESRGLAAALIAVGPDLD
                     YLTGYRAMPLERLTMLAVVAGEVPVLVVPRLEEPAARAGAQTPIEIATWMETDDPHAL
                     VAGLVDRARGRASATIAVSDRLWSLQLLRLQSAAPGARWVSATEVLRELRMVKDADEI
                     ELLRLAGAAADRVVDQISSGRLVGRTEADVAREVRERLLVEGHELAEFAIVASGPNSA
                     SPHHEASDRLIQAGEPIVLDIGGALGGYGSDTTRTLWVTGGDDGNGPTEEFRHLFGVL
                     LSAQVAATRAVRPGVAAEAIDKTARDIIDGEGYAE"
BASE COUNT          481 a         1145 c         1077 g          468 t
ORIGIN      
        1 cgagaaggtc ctccgcagct atcgccgggt cctgatcccg gaggtcaacc tcggccagct
       61 gctcatgctg atccggagcc agttcctcat cgacgccgag ggctacgacc gggtccgcgg
      121 caagccgttc acgatcgccg agatcgtgac ccaggccgag cgaatcctcg ggccgatggc
      181 gtcgcggagc tccgcctcgg acctcggaga gcgagcgaac gatcatgact gagggtgcca
      241 acccgatgac ggacgcgacc cagccggccc tcagcgcggc gaacgcggcc gtcatccagc
      301 tgacccgcaa ggacttcgtg tccgaccagg aggtccgctg gtgtcccggc tgcggcgact
      361 actcgatcct cgcccagacc cagaagcaga tgcccgactt cggcgtgccg aaggagaaca
      421 tcgtgttcat cagcggcatc ggttgctccg gccggctgcc gtactacatg aacacgtacg
      481 gcttccacac gatccatggc cgcgccccga cgctggcgac cgggctcaag gcggcccgtc
      541 cggagctgat ggtctgggtc atcaccggcg acggcgatgc cctgtccatc ggtggcaatc
      601 acgtgatcca cacgatgcgc cgcaacgttg acatcacgat ggtcatgttc aacaaccgga
      661 tctacgggct gaccaagggc caggccagcc cgacctcgga gttcggcaag aagacgaagt
      721 cgaccccggc cgggaccatc gacaccccga tcatgccgct gacgatcgcc ctggccgccg
      781 aggcgacgtt tgtcgcccgg agcgtcgaca cccacaccga gcatctccag cagaccctcc
      841 agcgggcggg gatgcatcac ggctcgacct tcgtcgaggt cctccagaac tgcaacatct
      901 tcaacgacgg cgcatggcgc gacttcaccg atcgcgaggt ccgcgaggat cggatgctct
      961 tcctggagca cgccaagcca atgatcttcg gcaaggatcg ggacaagggc attcgcctcc
     1021 gtgggctcca tcccgaggtg gtgacgatcg gcgagaacgg cgtgaccgag gcggacctcc
     1081 tcgtccacga cgagcaggcc gaggagccgt atctcgggct gatgctgtcg cggatgttct
     1141 ggcccgaata cccggtcccg gtcggcgtcc tccgcgccgt cgcgcgaccg acccacgatc
     1201 agttgatcga ccagcagatc tccgcggccg tcgccgcgac cggtgcgggc gacctcgccg
     1261 aggcgctcgc gtccggcgag acctggctcg tcgagtagcc ggaggatcgc gatgcgttgc
     1321 ccgacctgtg gatacgagaa cctgctcggc tccgacgtgt gcgacaactg cggctcggac
     1381 ctcgccggtc acgacgtccc gcagtcgccg tcgacgttcc gcggccagct gctcggcgag
     1441 cacctcgatg cgctcgaaat cgtcgccccg gagacgatcg aggcgaccgc cgacgtcaac
     1501 gaggccatcc gccgaatgca cgacaacgcg atcgactgcg tcctcgtcac cgaggccggg
     1561 cggctcgtcg ggatcttcac ggaccgcgat gcggtggtga aggtcgccgg cctcgaacgc
     1621 cgggtgcgcc cgatcgcgac cctgatgacc cgcgacccgg tcgtcctccg ccatgacgac
     1681 acgatcgcgg tcgcgatcaa caagatggcg ttcggcggct tccgccatat cccgatcgtc
     1741 gaggacggcc ggcccaccgg cgtcgtgacc gcccgcgacg tgttccgtca cctcgtcgag
     1801 tcgctcggct gacgccgggc ggtccatgac catggtcgag tccggagcgt cgatccgcgt
     1861 cgtggtgctc gccgacgacc tgatctggtc gacccgcctc gtcggccacg tccgggcggc
     1921 cggcttcgag gcggtgccgg cacgctcgat caacggattc ctgtcgggac tggccgacgc
     1981 cggcggtgcg atcgtggacc tgaccagccg ggcctacgac ggcctagcgg cgatcgccgc
     2041 ggcccgcgcc gccggcgtgc cggtcctcgc cgtcggccag cacgacgatc acgtccttcg
     2101 gcgcgacgcg ctcgcggtcg gcgccgaccg cgtcctggcc tatcgcaagc tgttcgagga
     2161 cggcccggcg acgatcgcgc gctggttggc ggcgtcttcg tcgacccctt cccgccagcc
     2221 gacggagacc acgacccgat gacgaccgcg accgcggccc atccccggat tccggccgag
     2281 cgctatgccg agcggctgca acggctcgcc gcgctggtcg agagccgcgg cctcgctgcg
     2341 gccctgatcg cggtcggacc ggatctcgac tacctgaccg gctatcgggc gatgccgctc
     2401 gagcgcctga cgatgctcgc ggtcgtcgcc ggcgaggtgc cggttctggt cgtgccccgt
     2461 ctggaggagc ccgcggcccg ggccggggcg cagacgccga tcgagatcgc gacgtggatg
     2521 gagaccgacg atccgcacgc cctcgtcgcc gggctcgtcg atcgagcgcg cggccgtgcc
     2581 tccgccacga tcgcggtctc cgaccggctc tggtccctcc agctgcttcg gctccagtcg
     2641 gccgcgcccg gcgcgcgctg ggtgtcggcg accgaggtcc tgcgcgagct gcggatggtc
     2701 aaggacgccg acgaaatcga gttgcttcgt ctggccggtg ccgccgccga tcgggtcgtc
     2761 gaccagatct cctccggtcg gctcgtcggg cggaccgagg ccgacgtcgc ccgcgaggtc
     2821 cgcgaacgcc ttctcgtcga gggtcacgag ctggcggagt tcgcgatcgt ggcgtccgga
     2881 cccaactcgg catccccgca ccacgaagcg tcggaccggc tgatccaggc cggcgagccg
     2941 atcgtgcttg acatcggcgg cgcgctcggc ggctacggta gtgatacgac ccgcaccctg
     3001 tgggtgaccg ggggcgacga cggcaacggg ccgaccgagg aattccgcca cctgttcggc
     3061 gtgctcctct cggcgcaggt cgcggcgacg cgggcggtgc gcccgggggt ggccgcggag
     3121 gcgatcgaca agacggcccg cgacatcatc gacggcgagg gctacgccga g
//
LOCUS       DATAIW010000183         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_242416, whole genome shotgun sequence.
ACCESSION   DATAIW010000183 DATAIW010000000
VERSION     DATAIW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242416"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VHM48_05905"
     CDS             <1..639
                     /locus_tag="VHM48_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2754976.1"
                     /translation="ATGRALDWFRVDVLRGATTAATLIEEAGAIPAGADGVVFLPYLA
                     GERSPLWDPTARGAFVGLGLGHGRGHLARAILEASAFAIRHVAEPILAAGAEVRAMRV
                     CGGPARSETWNQIKADVTGFTVEVPAVLETAVAGSAILAATGVGAWPDLPAAIRGMTR
                     SARRLAPNPAHRARYDATYDAYRRLHPAIAPIVRDLNAPAEGAAAAAPGPAR"
     gene            636..1445
                     /locus_tag="VHM48_05910"
     CDS             636..1445
                     /locus_tag="VHM48_05910"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2754977.1"
                     /translation="MTGPLPAPSTRPGRLVADDIRVAFRPPSRGAPPTVALDGLSLDV
                     AGGEIVALIGPNGCGKSTFLRVAAGLLAPERGRITLDNRPIAAPDDRIGLVFQEPRLL
                     PWRSVAQNITYPLELAGWSADRRATRLAELLHAVGLEAAAGLRPVQMSGGMRQRAAIA
                     RALALSPDVMLLDEPFSSLDALTRERFNLELLRLQERTGTTIVIVTHSIPEAILVADR
                     VIVMTPRPGRVAAEVRIDAPRPRSVELLDEALVSRAAAEIRAHLELSENVA"
     gene            1442..2296
                     /locus_tag="VHM48_05915"
     CDS             1442..2296
                     /locus_tag="VHM48_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2754978.1"
                     /translation="MSRDLTSTPDRPPTTVVPPRPADPVPSPIEGSRAFGRTVAVLWP
                     GVVAFVAFVAGWKLVAVIAGLGFILPPPEEVGARFIAAWADGTMEPHAWTTIVEVFLG
                     FAAGAGAGLAVGYVIARSHLVERLVSPYLVAAQAVPILALAPLLALWFGPGLLANVVI
                     CGLIVFFPVAIATMVGIRSVDRRLIELGRSLRASRRQVLVTLEIPAALPSIMGGLRVG
                     VTLAVVGAIVGEWAGADRGLGVLVNLARASLFDYPLMFATLVTIALLGIACYSLVVLA
                     ERRLVGLR"
     gene            2334..3359
                     /locus_tag="VHM48_05920"
     CDS             2334..3359
                     /locus_tag="VHM48_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2754979.1"
                     /translation="MPRRQTITARSIGVVALVGAVSAALVGACSPSASATATKLNVGL
                     GYIPSVQFAAFYRADQAGYYAAEGLAVTFQNAIDADLVPKVGAGTLDLGLSDGTSVIP
                     AVSQGIPIKYVATVYAKFPSIVFAKGSSGITDANGLVGKKLGIPGKYGSSWIMLQALL
                     ASKNHTTSDLTIVEYPQFTQGAAVSQGAVDAATGFDNNEPVQLALTGAQVTILRIDDV
                     TPLPGPGIIVGTATIASKKGAVAGFVRATLKAMNEIATSPSIGVDAAIKAVPDLAKNR
                     PLQEAVLAATIADWRPSGAAAGSPITGAVDAAAWDKTLTFMTTLGLVPKPVTTTDLVD
                     TSFAPGS"
     gene            3446..3784
                     /locus_tag="VHM48_05925"
     CDS             3446..3784
                     /locus_tag="VHM48_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2754980.1"
                     /translation="MPFRQIGSQLVKGTTPLLVLTVIGEGELYGYEIAQAVRERSGGA
                     FAPSEGSLYPTLHRLELDGALVADWRASDRGPRRRYYKLTKAGRGMLAESRTEWSALV
                     RAVDAVSSGG"
     gene            3787..5088
                     /locus_tag="VHM48_05930"
     CDS             3787..5088
                     /locus_tag="VHM48_05930"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX2754981.1"
                     /translation="MIGGADREVAGFAAGADGAGAGDHYLERVAARLRMPADAERDVI
                     DELRAHLDDARAALEAEGLDPDKAEREAMARLGSPDALGDDLRRTHHTRRRLIAAAGG
                     GVWDGLKDAITGYIVGIIVVLIVFMGLGTLAQRLLGPASLNAFGGRGVETAYSGALFS
                     IAAWWGARGLVRSMARRSLRRTAALRAPIALAGALPLTVLLLVWPLHYNWGGIAAMLA
                     LPVSWAVAALTTPEPGRRSRWSFGRPSPRLLGGGAIAVFVVPLALYLISPVTTVTTNQ
                     SWAADDVLARMPDQEHWVALGYNVVAPTVVDLQRSRWTSTPFPDRNGNVALSLDNDTI
                     DWDELADLRAEAWAALPSNRWVETLDPRAVGPYAVVPLQPWAATAAIVSVGAKPGIDA
                     YLVFVTGVDRATGKRVAIGYPEGEEVAFTGTIVDWFAWLGP"
     gene            5090..5884
                     /locus_tag="VHM48_05935"
     CDS             5090..5884
                     /locus_tag="VHM48_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754982.1"
                     /translation="MRLIRPSLFAFTAILVFGIPLAFGFLNWRNAERTGYLWNADAKP
                     HDVRVIDLWSGAVVQVGRIGPRSMVLLFHGWPDEWWVNPDTAGDPRRHDLRIEVLGAG
                     CELVSAYRAVTEADGTSNVTEISEGGDVAETEWPTQLSRSKAGALAAIADPCGDATPE
                     PRGVVVNSTRVAVDVGHGLVVPACHAVVVHPGDLLDGSVADPPTPKGAVRVAIPSMDA
                     QDERWPIEPRSVTIAADQIDDSGWVYDGSPVGPCAGRPKPGATADE"
BASE COUNT          746 a         2269 c         2121 g          884 t
ORIGIN      
        1 gcgacgggtc gcgccctcga ctggttccgc gtcgacgtcc tccggggcgc gacgacggcc
       61 gcgaccctga tcgaggaggc gggcgcgatc ccggccggcg ccgacggagt cgtcttcctg
      121 ccgtatctcg caggcgagcg ctcgccgctg tgggacccga ctgcccgcgg cgcgtttgtc
      181 ggactggggc tcgggcacgg gcgcgggcac ctcgcccggg cgatcctcga ggcgtcggcc
      241 ttcgccatcc gccacgtggc cgagccgatc ctggccgccg gcgccgaggt ccgggcgatg
      301 cgggtctgcg gcggaccggc tcggagcgag acctggaacc agatcaaggc agacgtgacc
      361 ggcttcaccg tcgaggtgcc ggccgtcctc gagaccgcgg tcgcgggctc ggcgatcctg
      421 gcggcgacgg gcgtcggagc gtggcccgac ctgccggccg cgatccgagg catgacccgg
      481 tcggcgcgcc ggctcgcccc gaacccggcg catcgtgccc gctacgacgc gacgtacgac
      541 gcctatcgac ggctccaccc ggcgatcgcc ccgatcgttc gcgacctcaa cgcaccagcc
      601 gagggcgccg cggcggccgc gccgggtccc gcccgatgac cggcccgctg cccgctccgt
      661 cgacccggcc gggccgcctc gtcgccgacg acatccgcgt cgcgttccgg ccgccgtccc
      721 gcggcgcgcc gccgaccgtg gccctcgacg gcctctcgct cgacgtcgcc ggcggcgaga
      781 tcgtggccct gatcggcccg aacggctgcg gcaagtccac gttcctgcgg gtcgccgccg
      841 gtctcctcgc tcccgagcgc ggccggatca ccctcgacaa ccgcccgatc gccgcgccgg
      901 acgaccggat cgggctcgtc ttccaggagc cgcgactcct gccgtggcgc tcggtggcgc
      961 agaacatcac gtacccgctc gagctcgccg gctggtcggc cgaccgacgg gcgacccggc
     1021 tggcggagct cctccacgcg gtcgggctcg aagccgcggc ggggctccgc ccggtccaga
     1081 tgtcgggcgg catgcgccag cgggcggcga tcgcgcgggc gctcgccctg tcaccggacg
     1141 tgatgcttct cgacgagccg ttcagctccc tcgacgccct gacccgtgag cgcttcaatc
     1201 tggagctgct ccgcctccag gagcggaccg ggacgacgat cgtcatcgtc acccacagca
     1261 tccccgaggc gatcctcgtc gccgaccggg tgatcgtcat gaccccgcga cccggccgcg
     1321 tcgccgcgga ggtccggatc gacgccccgc ggccgcgctc ggtcgagctc ctcgacgagg
     1381 ccctcgtgtc gcgcgccgcg gccgagatcc gggcccatct cgagctctcg gagaacgtgg
     1441 cgtgagccgg gacctcacat cgacacccga ccgtccgccc acgacggtcg tgccgccccg
     1501 gcccgcggac cctgtgccgt cgccgatcga gggcagccgc gcgttcggtc ggacggtcgc
     1561 ggtcctctgg cccggagtcg tcgccttcgt cgccttcgtg gccggctgga agctcgtcgc
     1621 cgtgatcgcc gggctcgggt tcatcctccc gccgcccgaa gaggtcggcg ctcgcttcat
     1681 cgccgcctgg gccgacggaa cgatggagcc gcacgcctgg acgacgatcg tcgaggtgtt
     1741 cctcggcttt gccgccggcg ctggcgccgg gctcgccgtc ggttatgtca tcgcccgctc
     1801 ccatctcgtc gagcgcctcg tgtcgccgta tctcgtcgcc gcccaggccg tcccgatcct
     1861 cgccctcgcc ccgctcctcg cgttgtggtt cggccccggc ctgctggcca acgtcgtgat
     1921 ctgcggcctg atcgtgttct tcccggtcgc gatcgcgacg atggtcggga tccgctcggt
     1981 cgaccgccgg ctgatcgagc tcggccggtc gctccgcgcc agccgccgcc aggtcctggt
     2041 cacgctcgag atcccggccg ccctgccctc gatcatgggc ggccttcgcg tcggggtcac
     2101 gctcgccgtc gtcggcgcga tcgtcggcga gtgggccggg gccgacaggg gtctcggcgt
     2161 gctcgtcaac ctggctcgcg ccagcctgtt cgactacccc ctgatgttcg cgaccctggt
     2221 gacgatcgcg ttgctcggga tcgcctgcta ttcgctcgtc gtgctcgccg agcgccgcct
     2281 cgtcggcctc cgctgaggcg cctcccatgc cccgtcaacc cgaggagaat cgcgtgcccc
     2341 gtcgtcagac catcacggct cgatccatcg gtgtcgtcgc gctcgtcggc gccgtcagcg
     2401 ccgccctggt cggcgcttgt tcgccgagcg cgtccgccac cgcgaccaag ctcaacgtcg
     2461 ggctcggcta catcccgagc gtccagttcg ccgcgttcta ccgagctgac caggccggct
     2521 actacgccgc cgagggactc gcggtcacgt tccagaacgc gatcgatgcg gacctcgtcc
     2581 cgaaggtcgg ggcgggcacg ctcgatctcg gcctgtcgga cgggacgagc gtgatccccg
     2641 cggtcagcca gggcattccg atcaagtacg tcgccacggt ctatgccaag ttcccctcga
     2701 tcgtgttcgc gaaggggagc agcggcatca ccgacgcgaa cgggctcgtc ggcaagaagc
     2761 tcgggatccc cggcaagtac ggctcctcgt ggatcatgct ccaggccctc ctcgcgtcga
     2821 agaaccacac gaccagcgat ctcacgatcg tcgagtatcc gcaattcacc cagggcgccg
     2881 cggtcagcca gggcgccgtc gacgccgcca ccgggttcga caacaacgag cccgtccagc
     2941 tcgccctcac cggggcgcag gtgacgatcc tgcggatcga cgacgtgacc ccgctgccgg
     3001 gccccgggat catcgtcggg acggccacga tcgcgtcgaa gaagggcgcc gtcgcgggct
     3061 tcgtccgggc gacgctcaag gcgatgaacg agatcgcgac ctcgccgtcg atcggcgtgg
     3121 atgcggcgat caaggccgtg cccgacctgg cgaagaaccg gccgctccag gaggccgtcc
     3181 tcgcggccac gatcgccgac tggcgcccga gcggcgccgc ggccggatcg ccgatcacgg
     3241 gcgccgtgga cgcggcggcc tgggacaaga cgctgacgtt catgacgacg ctcggcctcg
     3301 tcccgaagcc ggtgacgacg accgacctcg tcgacacgtc cttcgcgccg gggtcgtagg
     3361 gccgtcgcga aacagccctt gacggcgggt cgggcgagcc tcatcctcgc gttgtgctat
     3421 atcgatatcc gaggtagctc ccgcgatgcc gttccgccag atcgggtccc agctcgtcaa
     3481 gggcacgacg ccgctcctcg tcctgaccgt catcggcgag ggcgagctgt atggctacga
     3541 gatcgctcag gccgtccgcg agcggagcgg cggcgcgttc gccccgtccg agggctcgct
     3601 ctatccgacc ctccatcgac tcgagctcga cggcgcgctc gtcgcggact ggcgggcgag
     3661 cgatcgcggt ccgcgccgcc ggtattacaa gctcacgaag gcgggccggg ggatgctcgc
     3721 cgagagccgg acggagtggt ccgcgctcgt ccgggcggtc gacgccgtgt cgtccggcgg
     3781 ctgacgatga tcggaggggc ggatcgcgag gtcgccgggt tcgcggcggg cgcggacggc
     3841 gccggcgccg gcgaccacta cctcgagcgg gtcgcggcgc gcctccggat gccggccgat
     3901 gccgagcgcg atgtcatcga cgagctgcgc gcccatctcg atgacgcgcg cgcggccctc
     3961 gaggccgagg gcctcgaccc ggacaaggcc gagcgcgagg cgatggcccg gctcgggtcg
     4021 cccgacgcgc tcggcgacga cctccgtcgg acccaccaca cgaggcgtcg gctcatcgcc
     4081 gctgcgggcg gcggggtctg ggacggcctg aaggacgcga tcaccggcta catcgtcggg
     4141 atcatcgtcg tgctgatcgt gttcatgggc ttggggacgc tggcccagcg gttgctcggc
     4201 ccagcgagcc tcaacgcgtt cggcgggcgc ggcgtcgaga ccgcctactc cggtgcgctc
     4261 ttctcgatcg ccgcgtggtg gggcgcgcgc ggcctggtcc ggtcgatggc ccgccgctcg
     4321 ctccggcgca cggccgccct ccgcgccccg atcgcgctcg ccggggcgct cccgctcacg
     4381 gtcctgctcc tcgtctggcc gctccactac aactggggcg ggatcgcggc gatgctggct
     4441 ctgcccgtgt cgtgggccgt cgcggcgctc acgaccccgg agcccgggcg ccggtcgcgc
     4501 tggtcgttcg gccgtccatc gccgcggctg ctcggcggag gggccatcgc ggtgttcgtc
     4561 gtgcccctcg ccctgtacct gatcagcccg gtcaccacgg tgacgacgaa ccagtcctgg
     4621 gccgccgatg acgtcctggc gaggatgccc gatcaagagc attgggtcgc gctcggatac
     4681 aacgtcgtcg cgccgaccgt cgtcgacctc cagcggagcc gctggacctc gacgccgttt
     4741 cccgaccgca acggcaacgt cgcgctgagc ctcgacaacg acacgatcga ctgggacgaa
     4801 ctggccgacc tccgggccga ggcctgggcg gcgctgccga gcaaccggtg ggtcgagacg
     4861 ctcgatccac gggcagtcgg cccctacgct gtcgtgccgc ttcagccatg ggcggcgacc
     4921 gccgccatcg tgtcggtcgg cgcgaagccc ggcatcgacg cgtacctcgt cttcgtgacg
     4981 ggcgtcgacc gggcgaccgg caagcgggtg gcgatcggct accccgaggg cgaggaggtc
     5041 gcgttcacgg gcacgatcgt cgactggttt gcgtggctgg ggccatgaga tgcgcctgat
     5101 ccggccgtcc ctgtttgcct tcaccgccat cctcgtgttc gggatcccgc tggccttcgg
     5161 gttcctcaac tggcgcaatg ccgagcggac aggctatctc tggaacgcgg acgcgaagcc
     5221 gcacgacgtg cgggtcatcg acctgtggtc cggcgccgtg gtgcaggtcg gtcggatcgg
     5281 accgcgctcg atggtgctcc tcttccacgg ctggcccgac gagtggtggg tcaacccgga
     5341 cacggccggc gacccccgcc gccacgacct ccggatcgag gtcctcgggg ccggctgcga
     5401 gctcgtgtcc gcctatcggg cagtgaccga ggccgacggc acctcgaacg tgacggagat
     5461 ctccgagggc ggcgacgtgg cggagacgga gtggcccacc cagctgtcgc ggagcaaggc
     5521 cggggcgctg gccgcgatcg ccgacccgtg cggcgacgcg acgcccgagc cgcgtggcgt
     5581 cgtcgtgaac tcgacccgcg tcgcggtgga tgtcggtcac gggctcgtcg tgccggcctg
     5641 ccatgccgtg gtcgtccacc cgggcgacct cctcgacggc tcggtcgccg atccgccgac
     5701 gccgaagggc gccgtccggg tcgcgatccc gtcgatggac gcccaggacg agcgctggcc
     5761 gatcgagccc cgcagcgtca cgatcgccgc cgaccagatc gacgactccg ggtgggtcta
     5821 cgacgggtct ccggtcgggc cgtgcgcagg ccgcccgaag cccggcgcga ccgcagacga
     5881 ataggcaaca gcgctcccgg agcgcgagac gcccgtcggc cgggagcggc cgccctatga
     5941 tcggccgatg agcgagaccg tgagccccct gccggccggc ctctcgtccg cgccgccgtt
     6001 cagcctgcgg gcacgagtcg
//
LOCUS       DATAIW010000184         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_98681, whole genome shotgun sequence.
ACCESSION   DATAIW010000184 DATAIW010000000
VERSION     DATAIW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98681"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHM48_05940"
     CDS             <1..349
                     /locus_tag="VHM48_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754983.1"
                     /translation="TPVVVLDEPTTGQDARGIERVHRIVADLSSAGRTVIAISHDMRF
                     VAEAFERIIVMRDARIVLDCPPAEVFAEAAWPTLASTFLEPPLAARVGARLGLGSTPT
                     DEALLSALSTAST"
     gene            complement(619..>1647)
                     /locus_tag="VHM48_05945"
     CDS             complement(619..>1647)
                     /locus_tag="VHM48_05945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX2754984.1"
                     /translation="CKGSGQFDPIIEVVPGPGTNAGAVYASYMNGYNIMFVKSTNHGS
                     TWSAPVPVFGNVSWNDKPTIAVSNNGVDVYISFNGPTGGDPWMAQSHNSGATWTQTKL
                     VDSGRYYFDFDSDVAADGTVYFAETSILYGGGGNKGTTPSGQIEEHVFISRDRGATWE
                     NRTIASVWPGIACVAAGCTPDYYLGHIALSVDGSNNVVALYDGAPAAGGLQTIEARRS
                     TNGGVSWSAPVTLSAAGETTDAPAVESRANGDVRAWYYQTSGGGNVDAWNVWYRSSAD
                     GGATWSAAVDIADASGGAAYKTAAGFAEVYGDYGEIGITNTGKSIAIWGEGLSYTGPG
                     GVWFNRQP"
BASE COUNT          235 a          590 c          549 g          273 t
ORIGIN      
        1 cacaccggtc gtcgtgctcg acgagccgac gacgggccag gacgcgcgcg gcatcgaacg
       61 ggtccaccgg atcgtcgccg acctgtcctc ggccggtcgg accgtcatcg ccatcagcca
      121 cgacatgcgc ttcgtggccg aggcattcga gcggatcatc gtcatgcgcg atgcccggat
      181 cgtcctcgac tgcccgcccg ccgaggtgtt cgccgaggca gcctggccga ccctggcgtc
      241 gacgttcctc gagccgccgc tggccgcgcg ggtcggtgcc cggctcggcc tgggttcgac
      301 cccgacggac gaggcattgc tttcggcgct ctcgacggcg tcaacctgac cccgctgatg
      361 cctggcaagc attgatcccg gaggcggccc ggctcggatg ggccgacgaa cggccgtcgc
      421 cgcggaatcc agccagagaa ccggcccggt gatgtctatc cggcatcgat ctgcgccgta
      481 tgcgccgtat gcaccgagcg atggcaatct cagcgatcgc tcggtcaaga aacaatgtct
      541 gtcagacatc gccgcacggt ccgcacgttg tgcacggtcc gcacggtccg cacggtcgca
      601 gccgcccatc gcgatccgct acggctggcg gttgaaccag actccgccgg gcccggtgta
      661 gctgagtccc tcgccccaga tcgcgatcga cttgcccgtg ttcgtgatcc cgatctcgcc
      721 gtagtcgccg tagacctcgg cgaagcccgc cgcggtcttg tacgccgcgc cgccgctggc
      781 gtcggcgatg tcgaccgccg ccgaccacgt cgcgccgccg tccgccgagc tccggtacca
      841 gacgttccag gcgtcgacgt tgccgccgcc cgaggtctgg tagtaccagg cccggacgtc
      901 gccgttggcg cgcgactcga ccgccggggc gtccgtcgtc tcgccggccg ccgacagggt
      961 cacgggcgcg ctccaactca cgccgccgtt ggtcgatcgg cgggcctcga tcgtctgcag
     1021 gccgccagcc gccggtgcgc catcgtagag ggcgaccacg ttgttcgagc cgtccacgga
     1081 cagggcgatg tggccgaggt agtagtcggg cgtgcagccg gcggccacac aggcgatccc
     1141 gggccagacc gatgcgatcg tccggttctc ccacgtcgcc ccccggtcgc gcgagatgaa
     1201 gacgtgctcc tcgatctggc cgctcggcgt cgtgcccttg ttgccgccgc cgccgtacag
     1261 gatcgaggtt tcggcgaagt agacggtccc gtccgcagcc acgtcggaat cgaagtcgaa
     1321 gtagtaccgg ccggagtcga cgagcttggt ctgcgtccac gttgccccgg aattgtgcga
     1381 ctgggccatc cacgggtcgc cgccggtcgg gccgttgaac gagatgtaga catcgacgcc
     1441 gttgttgctg accgcgatcg tcggcttgtc gttccacgac acgttgccga agaccgggac
     1501 cggcgccgac caggtcgagc cgtggttcgt cgacttcacg aacatgatgt tgtagccgtt
     1561 catgtacgag gcgtagaccg cgcccgcgtt cgtccccggt cccgggacga cctcgatgat
     1621 cgggtcgaac tggcccgagc ccttgca
//
LOCUS       DATAIW010000185         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_69297, whole genome shotgun sequence.
ACCESSION   DATAIW010000185 DATAIW010000000
VERSION     DATAIW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69297"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VHM48_05950"
     CDS             complement(<1..955)
                     /locus_tag="VHM48_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2754985.1"
                     /translation="MDRDPAGVPNVPHAGEPPAAQPLTDILLRPAARSSRPPQAPPEP
                     ARAQVEITIDGAPVAVPAGSTILEACGAEGIDTPTLCYLENLTPVNVCRVCVVEVAGS
                     RVLVPACSRKVEAGMDVKTDSERVRHSRKMVLEFLGSSVDLSTAPAAAGYIERYGADP
                     ARYGPPSAPAAAGERDTHEAGHHHPPPTDASQTVAQPVKVDNDLYVRDYSKCILCYKC
                     VEACGEDAQNTFAIAVAGRGFDARISTEAAVSLPDSACVYCGNCIGVCPTGALMFKSE
                     FDMRAAGTWDESAQTVTETICPYCGVGCALELHVQDNTIVTV"
     gene            complement(973..2817)
                     /locus_tag="VHM48_05955"
     CDS             complement(973..2817)
                     /locus_tag="VHM48_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2754986.1"
                     /translation="MDLHVIGPLASPAERAAVDAVLGPAESGWRGGTRDTGLDGHVAR
                     GGHAARSRRDLLIPALHAVQSRIGWISQPALNYISRRLTVPPAEAYGVATFYALFATT
                     PRAPIVAHVCDDLACRLAGSEELCADLERTMGPAGAPDASGERTWLRSPCLGVCERAP
                     AAMITRAGTDAETTVVAPVDAAGIAARIGPEVAWWTEFRDHPVPQAGQAGLRLLARIG
                     VVDPTSLDDYRAHGGFRALERARAIGPQRVIDEVTAANLVGRGGAAFPTGRKWAAVAA
                     QAAQPHYLVCNADESEPGTFKDRVLMEGDPFAVVEAMAIEAFAVGAQKAYLYIRGEYP
                     LAEARIQGAIAASRGAGLLGDLDIELRRGAGAYICGEETALFESIEGKRGEPRNKPPY
                     PVEVGLFGKPTAINNVETLVNVLQIVGEDDGAAAFKAIGTDGSTGPKLFCVSGNVAYP
                     GVYEVEFGVTLGELVDLAGGLPRGRALKAILLGGAAGVFVGPEALDTPLTFEGTRAIG
                     ATLGSGVVMVFDDTADMVGALTRIARFFRDESCGQCVPCRVGTVRQEELLARLSSGVA
                     ATHGRRDDELALLGEIGQAMRDASICGLGQTASSAIESAVRRPDLVAL"
     gene            2904..3203
                     /locus_tag="VHM48_05960"
     CDS             2904..3203
                     /locus_tag="VHM48_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2754987.1"
                     /translation="MSEVTNQPARVGIRELRQNLSVYLERVRAGETLEVTDHGRPVAN
                     LEPIRPVELTGLALLEAEGAITPARSDHRTLPAPPSIPGRPLSEILQEMRDEDPR"
     gene            3200..3592
                     /locus_tag="VHM48_05965"
     CDS             3200..3592
                     /locus_tag="VHM48_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2754988.1"
                     /translation="MTSLAYVDASALVKLVLAEAESLEMVRWYVESDRVLTSRIGIIE
                     TRRAVARRPHDPVHLERVLSGIEVISVTPRLGDLAATINPAGVRTLDAIHLATALGIG
                     GSLTSFVTYDDRLADAARALGLPVVSSA"
     gene            3685..3909
                     /locus_tag="VHM48_05970"
     CDS             3685..3909
                     /locus_tag="VHM48_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754989.1"
                     /translation="MHHRRLAASTGLAGSCTTTAAGTCGIGSGAIARKVQAATFTVTA
                     VTSGTATYDPSANHDPDGDSNGTSIVVARP"
     gene            complement(3932..4408)
                     /locus_tag="VHM48_05975"
     CDS             complement(3932..4408)
                     /locus_tag="VHM48_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754990.1"
                     /translation="MTLGHRAVRTVDQRPDIVHARLLELAARLRDELPPIEAGTQAAT
                     ALGVSGRLDVEIVDRGPKRIELRTRQGRIRGDGAVDLAPEAGDRTNVTIAVEIRPEGF
                     AASLMLGVALKTMPNLERQVIDGLEANLDGLAIELARPDAEWDPAAWQPVGLPTRG"
BASE COUNT          650 a         1626 c         1640 g          653 t
ORIGIN      
        1 tcacggtcac gatcgtgttg tcctggacat gcagctccag cgcgcagccg acgccgcagt
       61 aggggcagat cgtctcggtc accgtctggg cgctctcgtc ccaggtcccg gcggcccgca
      121 tgtcgaactc gctcttgaac atcagcgcgc cggtcggaca gacgccgatg cagttgccgc
      181 agtacacgca ggccgagtcc gggagcgaga cggccgcctc ggtcgagatg cgggcgtcga
      241 agccgcgccc ggcgaccgcg atcgcaaacg tgttctgggc gtcctcacca caagcctcga
      301 cgcacttgta gcagaggatg catttcgagt agtcgcgaac gtacaggtcg ttgtcgacct
      361 tgaccggctg ggcgacggtc tgggacgcgt cggtcggcgg cgggtggtgg tgacctgcct
      421 cgtgcgtgtc gcgctcgccc gctgcggccg gtgcggacgg cggtccgtag cgcgccggat
      481 cggcgccgta gcgctcgatg tagcccgcgg cggcgggcgc ggtcgagagg tcgaccgagg
      541 agccgaggaa ctcgaggacc atcttccggc tgtggcgaac ccgctcggag tcggtcttca
      601 cgtccatccc ggcctcaacc ttgcgcgagc acgcgggcac gaggacgcgc gagccggcga
      661 cctcgacgac gcagacccgg cagacattga ccggggtcag gttctcgagg tagcagaggg
      721 tcggcgtgtc gatgccctcc gcgccacagg cctccaggat ggtcgagccg gccggaacgg
      781 ccaccggcgc gccgtcgatc gtgatctcga cctgtgctcg ggcgggctcc ggcggggcct
      841 gcggcggccg gctggaccga gcggccgggc ggaggaggat gtcggtgagc ggctgggccg
      901 cgggcggctc gccggcatgg ggcacgttcg gcacgccggc cggatcgcga tccatgctca
      961 gcccgtcgcc gctcacaggg ccaccaggtc cggacgtcga accgcgctct cgatcgccga
     1021 gctggccgtc tggccgaggc cgcagatcga ggcgtcacgc atggcctggc cgatctcccc
     1081 gagcagggca agctcgtcgt cgcgcctgcc gtgggtggcg gccacgccgg acgagagccg
     1141 ggcgaggagc tcctcctgtc gaaccgtgcc gacccggcag gggacgcact ggccgcacga
     1201 ctcgtcgcgg aagaagcggg cgatcctggt cagggcgccg accatgtcgg cggtgtcgtc
     1261 gaagaccatc acgaccccgg acccgagcgt cgctccgatc gcccgcgttc cctcgaacgt
     1321 cagcggcgtg tcgagcgcct cagggcccac gaagacgccg gccgcgccgc cgaggaggat
     1381 ggccttgagc gcgcgaccgc ggggcagccc gccggcgagg tcgacgagct cgccgagggt
     1441 cacaccgaat tcgacctcgt atacgcctgg gtacgccacg ttgcccgaga cgcagaacag
     1501 cttcgggccg gtcgagccgt cggtgccgat cgccttgaac gcggctgcgc cgtcgtcctc
     1561 gcccacgatc tggaggacgt tgacgagcgt ctcgacgttg ttgatcgcgg tcggcttgcc
     1621 gaacagcccg acctcgaccg ggtacggcgg cttgttgcgt ggttcgccgc gcttgccctc
     1681 gatcgattcg aagagcgcgg tctcctcgcc gcagatgtag gcgcctgccc ctcgccgcag
     1741 ctcgatgtcg aggtcgccga gcagacctgc gccgcggctc gccgcgatcg cgccctggat
     1801 ccgcgcctcg gccagcgggt actcgccgcg gatgtagaga taggccttct gggccccgac
     1861 cgcgaacgcc tcgatcgcca tcgcctcgac gaccgcgaac gggtcgcctt ccatgaggac
     1921 acggtccttg aaggtcccgg gctcggactc gtccgcgttg cagacgaggt agtgcggttg
     1981 ggcagcctgg gcagccaccg cggcccactt ccggccggtc gggaacgccg cgccgccgcg
     2041 tccgaccaga ttggcggccg tgacctcgtc gatcacccgc tgcgggccga tggcgcgggc
     2101 ccgttccaac gcccggaacc cgccgtgcgc ccggtagtcg tcgagcgacg tcgggtcgac
     2161 gacgccgatc cgggcgagga gccggagacc ggcctgcccc gcctggggga cgggatggtc
     2221 ccggaactcc gtccaccagg cgacctccgg cccgatccgc gcggcgattc cggcggcgtc
     2281 gaccggcgcc acgacggtgg tctcggcgtc ggtccccgcc cgggtgatca tggcggccgg
     2341 cgctcgctcg cacaccccga ggcagggcga gcgcagccac gtccgctcgc cggaggcatc
     2401 cggagcgccc gccggcccca tcgtccgctc gagatcggcg cagagttcct cggatcctgc
     2461 gagacggcag gccagatcgt cgcagacgtg cgccacgatc ggcgctcggg gcgtcgtcgc
     2521 gaacagggcg tagaaggtcg cgacgccata cgcctcggcc ggcggaacgg tcagccgccg
     2581 cgagatgtag ttcagggccg gctggctgat ccagccgatc cgcgactgga cggcgtggag
     2641 ggcagggatc aagaggtcgc gtcgcgagcg cgccgcatgc ccgcctcggg cgacatgccc
     2701 gtccaatccg gtgtcccgcg tcccgccgcg ccagcccgac tcggccgggc cgaggacggc
     2761 gtcgaccgcc gcccgttcgg ccggggatgc cagcgggccg atgacgtgga ggtccatcgc
     2821 cgcgagtgta gccgcgccgg tccgcctgcc gccgcgcatg cccgcgccgt ggcctgtgaa
     2881 tcgattgtgc taccatgtgc tacatgagcg aagtgaccaa ccagccggcg cgggtgggga
     2941 tccgagagct gcgtcagaac ctgagcgtgt acctggagcg cgtcagggcc ggtgagacgc
     3001 tcgaggtcac agaccacggc cgacccgtcg cgaacctgga gccgatccgg cccgtcgagt
     3061 tgaccggcct cgccctcctc gaagccgaag gagcgatcac accggcaagg tccgaccatc
     3121 gaacgcttcc ggcgccgcca tccatccctg gaaggccgct gtcggagatc cttcaggaga
     3181 tgcgggacga ggatcctcga tgacgtcgtt ggcctatgtc gacgcctcgg cgctcgtgaa
     3241 gctcgtcctg gctgaggcgg agtcgctgga gatggtgcgc tggtacgtcg aaagcgatcg
     3301 agtcctgacc agccggatcg gtatcatcga gactcgccgc gccgtcgctc gacgacctca
     3361 cgatccggtt catctggagc gcgttctgag cggaatcgag gtgatcagcg tcacccctcg
     3421 acttggtgac ctggctgcaa cgatcaaccc agccggcgtt cgcaccctcg atgcgatcca
     3481 tctcgcgacg gcgctcggca tcggggggag cctcacctcg ttcgtgacct acgacgatcg
     3541 gctcgccgac gccgcgcgcg ccctcgggct gccggtcgtc tcgtcggcct gagcgggtcc
     3601 ggcggggatt gccgccgcag cgattgcggc ataaggtccg gcggcgagtc cgaagccgga
     3661 cgccagttgc cagtggggga ccacatgcac caccgccgcc tcgccgcctc gaccggcctg
     3721 gccgggagct gcaccacgac ggcagccgga acgtgtggga tcgggagcgg cgcgatcgcg
     3781 cgaaaggtcc aggccgcgac attcacggtc accgccgtca ccagcgggac cgcgacctac
     3841 gacccgagcg ccaaccacga cccggacggt gactcgaacg ggacgtcgat cgtcgtcgcg
     3901 cgaccctgag gctcgtcgcc cccgccgtga ctcagccgcg ggtgggaagg cccacgggct
     3961 gccaggccgc cgggtcccac tccgcgtcgg gtctggccag ctcgatcgcg aggccgtcga
     4021 ggttggcctc gaggccgtcg atgacctgtc gctcgaggtt cggcatcgtc ttgagggcca
     4081 cgcccagcat caggctggcc gcgaagccct ccggcctgat ctcgacggcg atcgtgacgt
     4141 tcgtccggtc acccgcctcc ggcgccaggt cgacggcccc gtcgccgcgg atccgcccct
     4201 gcctcgtccg gagctcgatc cgcttcgggc cgcgatcgac gatctcgacg tcgagccggc
     4261 cgctcacccc gagcgccgtc gccgcctggg tgcccgcctc gatcggcgga agctcgtcgc
     4321 gcagccgggc tgcaagctcg agcaggcggg cgtggacgat gtccgggcgc tggtcgacgg
     4381 tccggaccgc gcgatgcccg agcgtcatgc ctcggcgatg atcgccatcg agatcaggag
     4441 gttgtcgacc tggagcgggt tgcgagcgcc ggagcgggcc ggcgggttcg tcgggaacca
     4501 gcgctgccac tcctcgttca tcccggcgaa gtcctcgggc cgggtcagga cgaaggtggc
     4561 gttcacgac
//
LOCUS       DATAIW010000186         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212586, whole genome shotgun sequence.
ACCESSION   DATAIW010000186 DATAIW010000000
VERSION     DATAIW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212586"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VHM48_05980"
     CDS             <1..786
                     /locus_tag="VHM48_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2754991.1"
                     /translation="LHNFADALTALPIWLAFIVGRRAATRRFSYGFGRAEDLAGLVVL
                     AFIAGSSVVAAYEAIDRLVHPAEVRYLWAVAIAGGIGFIGNELVAQYRIRVGRQIGSG
                     ALVADGLHARADGFTSLAVVAGAAGVALGFPAADPIAGLIIAVLILVVLKDAARDVLM
                     RIMDGVDPVIRGESEAVLAEVPGVEGVGEVRVRWIGHRLHAEAEITVDESRTVAEAHD
                     IAESARHALLHEVPHLASAIIHADPCGHGGSDPHAELAHHGAI"
     gene            910..984
                     /locus_tag="VHM48_05985"
     tRNA            910..984
                     /locus_tag="VHM48_05985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:942..944,aa:Met,seq:cat)
     gene            1188..>2017
                     /locus_tag="VHM48_05990"
     CDS             1188..>2017
                     /locus_tag="VHM48_05990"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX2754992.1"
                     /translation="MNPRFFRNGIVMLLLVVVALAVVLTVVNSTTPSSNTPYSVFLDN
                     VQSDKVASALQEGSTLTVTPKPDATKYTVVIPGLLVNQVLDDMTKAAATTKVPRPILE
                     AKQAPDNGWISILITGLLPILIIGGFIFFMMRQAQGTNNQALSFGKSRARMFLGNKTV
                     VTFADVAGVDEAKTELQEVVEFLKYPEKFNSLGARIPRGVLLVGPPGTGKTLMARAVA
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNSPCIVFVDEIDAVGRQRGAGL
                     G"
BASE COUNT          297 a          712 c          658 g          350 t
ORIGIN      
        1 ctccacaact tcgccgacgc cctcaccgcc ctcccgatct ggctggcctt catcgtcggc
       61 cgacgggccg caacccggcg cttctcgtac ggcttcggac gggccgagga tctcgccggc
      121 ctcgtcgtgc tcgcgttcat cgcgggctcg tcggtcgtcg ccgcgtatga ggcgatcgat
      181 cgcctggtcc acccggccga ggtccgctat ctgtgggccg tggccatcgc gggcggcatc
      241 gggttcatcg ggaacgagct cgtcgcccag taccggatca gggtcggccg ccagatcgga
      301 tcaggggcgc tcgtcgccga cggcctgcac gcccgcgccg atggcttcac ctcgctggcg
      361 gtcgtcgccg gtgcggccgg cgttgcgctc gggttcccgg ccgccgaccc gatcgccggg
      421 ctgatcatcg ccgtcctgat cctcgtcgtg ctcaaggacg cggcccgcga cgttctcatg
      481 cggatcatgg atggggtcga cccggtcatc cgtggcgagt ccgaggcggt tctcgccgag
      541 gtgccagggg tcgagggagt gggagaggtg cgcgtccggt ggatcggtca tcgcctccac
      601 gccgaggccg agatcaccgt cgacgagagc cggacggtcg ccgaagccca cgacatcgcc
      661 gaatcagcgc gtcatgcgct cttgcatgag gtcccgcatc tcgcatccgc gatcatccac
      721 gccgatccgt gcgggcatgg cggcagcgac ccgcacgccg agctggcgca tcacggagcg
      781 atatagccgc gccaacgctc cccacccgcg tcgctgagaa gcccactgtt atgtggtgtt
      841 cgatagttat gcacgctctt ggggcggtgc ttgccgctct ggatcacgct cgataagctt
      901 gatctcgttg gcggagtagc tcagtggtag agcaggggac tcataagccc ttggtcggtg
      961 gttcaaatcc gccctccgct accacccgac ctgatcgacg cccggcaccg cgacggttgg
     1021 ccgggcgtct cttcgttcga ccgcacatct gccggcgtcc cgcgggcgaa accctgtggt
     1081 gagcgggcga atgatccggc cggcccgtct cagggggccg tcatgaaccg acgctatact
     1141 ccacccgccg gtcgccgcgc tgcggcgcca ctgttccgga ggctccgttg aatcccaggt
     1201 tcttccgcaa cggcatcgtg atgctgctcc tggtggtcgt cgcgctcgcc gtcgtgctca
     1261 cggtcgtcaa ctcgacgacc ccaagcagca acaccccgta ttccgtattc ctcgacaacg
     1321 tccagtcgga caaggtggcg tcggcgctcc aggagggctc gacgctgacc gtcacgccga
     1381 agcccgatgc gaccaagtac acggtcgtca tcccgggcct gctggtcaac caggtcctcg
     1441 atgacatgac gaaggccgcc gccacgacca aggtgccccg gccgatcctc gaggcgaagc
     1501 aggcgccgga caacggctgg atctcgatcc tgatcaccgg gctgctgccg atcctgatca
     1561 tcggcgggtt catcttcttc atgatgcgtc aggcccaggg caccaacaac caggccctca
     1621 gcttcggcaa gagccgggcc cggatgttcc tcggcaacaa gaccgtcgtg accttcgccg
     1681 acgtcgccgg ggtcgacgag gccaagaccg agctccagga agtcgtcgag ttcctgaagt
     1741 acccggagaa attcaactcg ctcggcgccc ggatcccgcg cggggtgctg ctggtcggcc
     1801 ctccgggcac cggcaagacg ctgatggccc gggcggtcgc cggtgaggcc ggcgtcccgt
     1861 tcttcagcat ctccggctcg gagttcgtcg agatgttcgt cggcgtcggc gcgagccgcg
     1921 ttcgtgacct gttcgaccag gccaagcgca actccccgtg catcgtcttc gtcgacgaga
     1981 tcgacgcggt cggccgccag cgcggcgccg gcctcgg
//
LOCUS       DATAIW010000187         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_158805, whole genome shotgun sequence.
ACCESSION   DATAIW010000187 DATAIW010000000
VERSION     DATAIW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158805"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..1777)
                     /locus_tag="VHM48_05995"
     CDS             complement(470..1777)
                     /locus_tag="VHM48_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2754993.1"
                     /translation="MTDTAIPAVFPADLSTVADHITPVLGRYFERSWSHGEGHRLFDT
                     SGKAYLDFANGIAVTALGHHHPRVTAAIHAQVDRLIGPTGAMGFAEPVVRLADMLTAT
                     LPSSIDSLMFLNSGSEAIDGALKLARRVSGRPGIVAFRGGFHVRTFGATSVTTSSLNY
                     RTGYEPLLPGVYFAPYPAAWPEFDGAEERASEASLGVLRSLFATVIAPSQVGSIVIEP
                     VRGEGGYNPAPASFLQGLRRICDEHGILLIADEVQSGFGRTGRMWAFEHADIVPDVVV
                     LAKAIANGLPLSAIAASRDLMDRWGKGAHGSTYGGNPVACAAGIAVLETIAAEGLVEN
                     AAVRGAELRSGLERIAAEEPRIGDVRGPGLMVGVEFVIDPATREPDGATAKAAIARAA
                     DLGLLVLTCGSSSHVIRWIPPIDVTAAEISEAVEIFEETLRTI"
     gene            1886..>2145
                     /locus_tag="VHM48_06000"
     CDS             1886..>2145
                     /locus_tag="VHM48_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754994.1"
                     /translation="MTPDPLDLLRAARDDGSGGAMDAWAESGTAGLLVLRAFLEGRLR
                     LPAIGSSLHPRDAIDNTSKLVARIAEALEERRRRRVVTAFAA"
BASE COUNT          326 a          734 c          763 g          322 t
ORIGIN      
        1 gccgacatgc tgcatctcca cgtcaatacc gtgaagcggc tcggagatcg cggcgagctt
       61 ccgttctatc gggtctgcaa gcgcggcgac cgccgcttcc ggctcgatga cgtcatgaag
      121 ttcctcgccc agaaccgcta gctcacgggg cggctctgcc gcccgaacag aacgcgtcct
      181 ccatcgaggc gcccgctccg ccagcgggcg cttcgtcgcg tccgggtccg ctgcgcgata
      241 cgtccggttc gttggtgccg gacgtgttcc accaccgacc acgggccgcg ctggacgtca
      301 gcgatgtccg tccgcactcg tgtcacgcac cagcgcggat ggtgggtgac tccaatagcg
      361 cggttccgcc cgaaacggcg actgatccct gacttcgacc acccgggatg gtgattgtcg
      421 ggagatcgtt tcggtggacg gcgcaatccc gaggccggat cgccacccct cagatcgtcc
      481 ggagcgtctc ctcgaagatc tcgacggcct cgctgatctc ggcggcggtg acgtcgatcg
      541 gggggatcca gcggatcacg tgcgagctcg agccgcaggt cagcacgagc aggccgaggt
      601 ctgccgcccg ggcgatcgcg gccttggccg tggcgccgtc cggctcgcgc gtcgcggggt
      661 cgatcacgaa ttccaccccg accatcaggc ccggcccgcg gacgtcgccg atccgcggct
      721 cctccgccgc gatccgctcc aggccgctcc gcagttccgc gccgcggacc gcggcgttct
      781 cgacgaggcc ctcggcggcg atcgtctcga gaaccgcgat gccggccgca caggcgaccg
      841 gattgccgcc gtaggtcgac ccgtgggcgc ccttgcccca gcggtccatc aggtcgcgag
      901 aagcggcgat ggccgacagc ggcaggccgt tggcgatcgc cttggccagg acgacgacgt
      961 ccgggacgat atcggcatgt tcgaacgccc acattcggcc ggtccggccg aagccgctct
     1021 ggacctcgtc ggcgatcagc aggatcccat gttcgtcgca gatcctgcgg aggccctgga
     1081 ggaacgacgc cggcgccggg ttgtaaccgc cttcgccgcg cacgggctcg atgacgatcg
     1141 agccgacctg cgacggcgcg atgaccgtcg cgaacaggga ccggaggaca cccagggagg
     1201 cttcggacgc tcgctcctcg gcgccgtcga actccggcca ggcggccggg tacggggcga
     1261 agtagacgcc cggaagcaac ggctcgtagc cggtccggta gttcagggac gaggtcgtca
     1321 ccgatgtcgc cccgaacgtg cggacgtgga atccgccgcg gaacgcgacg atccccggcc
     1381 ggccggagac gcgccgggcg agcttgagtg ctccgtcgat cgcctccgag ccggagttga
     1441 ggaacatcag gctgtcgatc gacgacggca gggtcgccgt gagcatgtcg gcgaggcgga
     1501 cgaccggttc ggcaaagccc atcgccccgg tcgggccgat cagccggtcc acctgggcgt
     1561 ggatcgcggc cgtcacgcgc gggtggtgat ggccgagcgc ggtgacggcg atgccattgg
     1621 cgaagtcgag ataggccttg ccgctcgtgt cgaacaggcg gtggccctcg ccatggctcc
     1681 agctccgctc gaaatagcgg ccgaggacgg gggtgatgtg gtcggcgacg gtcgagaggt
     1741 cggccggaaa gacggccgga atggcagtgt cggtcatcga ccgagtatgc cagcgcgcct
     1801 gccccgattg ctgggccgtt cggcccggtg ggtggcgacg gagcgtctgc aggatggacg
     1861 tcgacccctg cgtcatgctt cagccgtgac acccgacccg ctcgacctcc tccgggctgc
     1921 cagggacgat ggctcgggcg gcgccatgga cgcgtgggcg gaatcgggaa cggcaggatt
     1981 gctcgtcctc cgggcgtttc tcgaaggccg cctgcgcctg cccgcgatcg gatcgagcct
     2041 gcatccgagg gacgcgatcg acaacacctc gaaattggtt gcgagaattg cggaggccct
     2101 ggaggaacga cgccggcgcc gggttgtaac cgccttcgcc gcgca
//
LOCUS       DATAIW010000188         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_212607, whole genome shotgun sequence.
ACCESSION   DATAIW010000188 DATAIW010000000
VERSION     DATAIW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212607"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VHM48_06005"
     CDS             complement(<1..728)
                     /locus_tag="VHM48_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2754995.1"
                     /translation="MEGFVAERYDAVIIGGGHNGLISAAYLARAGMKTLVLEQRHVLG
                     GAAVTEELFPGFRFSVFSYVVSLLRPEIIRELQLPRHGLDILPLDGTFTPLRPGEGPK
                     AGGGDYLWRVNDHGRTIRELRRWSAGDAEAYEEYGQLMVEMARFIKPILGITPPDLTS
                     LDPRPLLPLGGLIRSFQQLPERQQAVFVQLMTMSAADFLDQWFETDPLKATMSASGII
                     GTYLGVRSPGTAYVLLHHYMGEIDG"
     gene            912..1841
                     /locus_tag="VHM48_06010"
     CDS             912..1841
                     /locus_tag="VHM48_06010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX2754996.1"
                     /translation="MRKPLSIAVALLATLALAAPVAAITNGSPDAGEHPYVGELLFYV
                     PDVPSSRFTDPGGWFTCSGTLLSPTIVVTAGHCTFGVGLNSVSTTHNGADTDAAHGGV
                     GGNDVWISFAEVPDFNILPPSSTFTTQAARYAAWSSALNGSSEWHRATATPHPLYDDL
                     AFFVHDAGVLRLGSPVTMPTYGHVPTVDYLNRYRSQPRNEHRFETVGYGLEQVQGKKA
                     FGGDTRMQSHPKLDSLTSHPADTYMVLSNNTATGGTCFGDSGGPTFDDTSSNLVVAVT
                     SWGQSPNCGGKGGVYRLDQPDDQAFLAGFGITP"
BASE COUNT          328 a          748 c          695 g          304 t
ORIGIN      
        1 ccgtcgatct cgcccatgta gtggtggagc aggacgtagg cggtgcccgg cgagcgcacc
       61 ccgaggtacg tcccgatgat ccccgacgcg ctcatcgtcg ccttgagcgg atccgtctcg
      121 aaccactggt cgaggaagtc cgccgccgac atcgtcatca gctggacgaa gacggcctgc
      181 tggcgctccg gcagctgctg gaaggagcga atgaggccgc cgagcggcag cagcggccgc
      241 ggatcgagcg aggtcaggtc cggcggggtg atcccgagga tcggcttgat gaaccgggcc
      301 atctcgacca tcagctggcc gtactcctcg tacgcttcgg cgtcgccggc cgaccagcgg
      361 cgcagctcgc ggatcgtccg gccgtgatcg ttgacccgcc agaggtagtc gccgccgccg
      421 gccttcgggc cctcgcccgg tcggagcggg gtgaacgtcc cgtcgagcgg caggatgtcg
      481 aggccgtggc gcggcagctg gagctcgcgg atgatctcgg gccggaggag cgagacgacg
      541 tacgagaaga cgctgaaccg gaagccgggg aacagctcct cggtgacggc ggctccgccg
      601 aggacgtgac gctgctcgag gacgagcgtc ttcatcccgg cccgggcgag ataggcggcg
      661 ctgatcagcc cgttgtggcc gccaccgatg atgaccgcgt cgtagcgctc ggccacgaag
      721 ccctccatcc agcatgcata cgtttcatgc aatcgtagcc gagccgcgag cgcccgcgcg
      781 gcctcgttcc tcgtggtgcg gcgtgtcacc gaacggccga accaccggcc atcctcccga
      841 cgtactcccg gtaggatggc gcttgcccgg ccgttttccc ggccaacgcg cccctcctgg
      901 aggagagcac catgcgcaag cccctgtcca tcgcggtcgc gctcctggcg acgcttgcgc
      961 tcgccgcgcc ggtcgccgcg atcacgaacg gcagccccga cgcgggcgag cacccatacg
     1021 tcggcgagct cctgttctac gtgccggacg tccccagttc gcggttcacc gatccgggcg
     1081 ggtggttcac ctgcagcggg acactcctga gtccgacgat cgtcgtcacg gccggtcact
     1141 gcacgttcgg ggtcgggctg aacagcgtct cgaccaccca caacggcgcc gataccgacg
     1201 cggcgcatgg cggcgtcggc ggcaacgacg tctggatcag cttcgccgag gtgccggact
     1261 tcaacatcct gccgcccagc tcgacgttca cgacccaggc agcccgctac gcggcgtgga
     1321 gcagcgccct gaacgggagc agcgagtggc atcgcgcaac cgccacgccg catccgctct
     1381 acgacgacct cgcgttcttc gtccatgacg cgggcgtcct tcgcctgggc tcgccggtga
     1441 cgatgccgac atacggccat gtgccaaccg tcgactatct gaaccgctat cgctcccagc
     1501 cccgcaacga gcaccggttc gagaccgtcg gctacggcct cgagcaggtc cagggcaaga
     1561 aggcgttcgg cggcgacacg cggatgcagt cgcaccccaa gctcgacagc ctgacgagcc
     1621 atcccgccga cacctacatg gtcctctcga acaacaccgc gaccggcggg acctgcttcg
     1681 gtgactcagg cggaccgaca ttcgacgaca cgagctcgaa cctcgtggtc gcggtgacct
     1741 cgtggggcca gagcccgaac tgcggcggca agggcggcgt ctaccgcctc gaccagcccg
     1801 acgaccaggc gttcctcgcc gggttcggca tcacgccgta gccggcaagc gatcgctgcg
     1861 aagctcgaac gggccccgat ctcccggggc ccgttccttg cgcgcggacg tccgtgcggc
     1921 gcacggaatc ggcgaccgag tcggcaggga cgcgatcggc gcacgcccgc ccgtcgtcgt
     1981 cacgagtcgt cgtgacgcgg cgtcccacga gcgtgaagcg actcgctgcg gtcgtcctga
     2041 tcgccatcgt cgtcgcctgt agccccggag gttcg
//
LOCUS       DATAIW010000189        24669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_98990, whole genome shotgun sequence.
ACCESSION   DATAIW010000189 DATAIW010000000
VERSION     DATAIW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 24669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24669
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98990"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..399)
                     /locus_tag="VHM48_06015"
     CDS             complement(166..399)
                     /locus_tag="VHM48_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2754997.1"
                     /translation="MNLCIDIAPAFNGPDLDRDAGSLLGTDHGHPTAAGQDLIAATIG
                     PPDSCHFSDPGVAHGSPRMTTAPARVVPEGTFR"
     gene            503..1093
                     /locus_tag="VHM48_06020"
     CDS             503..1093
                     /locus_tag="VHM48_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2754998.1"
                     /translation="MEFADVVMKRRAVRRFEEGGVPRETLERIARLAQRAPSGGFSQG
                     QRLVVVTDPERRREIARICGEEWYEADFGPWISECAAQFVPCVSEQIYHRRYQEPDKL
                     QDDGTEIEWPIPYWWVDVGATMQNIWLAAVNEGLGCGFVGTDELDALRTSLGLPAEFT
                     PIGVMPVGIPVPDVRSPSLKRGWVPFDEFARWERWG"
     gene            complement(1235..2182)
                     /locus_tag="VHM48_06025"
     CDS             complement(1235..2182)
                     /locus_tag="VHM48_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2754999.1"
                     /translation="MSTRPVDVSARPHVAREHRLAELAVLGVMVLWAFNFIVVKSAVA
                     VLPPVGFTFLRFTIASTTLLILLRWREGSIGLPRRDLIAIGTLGALGFGVYQILWTTG
                     LKVIAAGDSALIIASTPVLVAFLAVMARSDVLTPGKLVGGIVSFAGVAAVIASGPGLS
                     LGGSLFGEAITLVAAICWAIYTAFGAPFLRRHSPLRTTAWATVAGTIVLAPIAASQLA
                     TVDASQLTPGVLGAVLYSGFLAAGVSNVIVLNGVRLIGPTRTSAYQFLVPALAVLLAF
                     LFLSEPIRPGQVVGGLVIVAGVLITRAGAPFRIVARPLR"
     gene            complement(2267..2422)
                     /locus_tag="VHM48_06030"
     CDS             complement(2267..2422)
                     /locus_tag="VHM48_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755000.1"
                     /translation="MKALKTVAVALGITLAALIVIPLIVLAGLFVWLKVTEEAEEDLL
                     ELDREGA"
     gene            complement(2480..2998)
                     /locus_tag="VHM48_06035"
     CDS             complement(2480..2998)
                     /locus_tag="VHM48_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755001.1"
                     /translation="MRIYEGSPRQDFEEVFRSIGAFIDQRGMREILLVEAPDGFIVQG
                     VVTAGAAGGSWSESMGSQSKETLTFLDDDIARFMEESVARRGKPGPEPVRDAGFYETA
                     LRVIGRYIDEQKPRDVFFFEQEGAFVVRLFLGGQAGSHHVLAEFTRDDIAQLVTQGPK
                     LRAPVMPKPAKS"
     gene            complement(3060..4031)
                     /locus_tag="VHM48_06040"
     CDS             complement(3060..4031)
                     /locus_tag="VHM48_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755002.1"
                     /translation="MTAVEPTLRPDAVGLASPEWGGFDMARTRGRGWIDVRRSFVTAV
                     RLGWLMEANWTDPLLFFIYSVAKPLASALILVVMLDVISGGAKPEYRAFVVVGSALWS
                     FVLSGVSGLAWMILDDRERYRMLKYVYVSPSDFLAVIFGRGVARIGVGAMGAAITLAV
                     GVVFLNVPFDIGRIDWPLLVVVMILGVTSILAIGLLLAAICMQTRQESWSYPEAAAGA
                     LFLVSGVVFPLAVLPTPVQALGLLTPLTWWIEGIRHALFPGGISAVGGPGALYTQLTG
                     AVSPSPAEIVLVLLATGAVATLGAVAVFRASDRRAKDRGLFDQTTGS"
     gene            complement(4036..4962)
                     /locus_tag="VHM48_06045"
     CDS             complement(4036..4962)
                     /locus_tag="VHM48_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755003.1"
                     /translation="MTAARDDGRRTIDAPGRTAFVYRPTASPQEAAIWTTRQVALTNL
                     RTVMGRAYPRVTGLFREKSWIAFEILLPFLATSAFVFVYRALQAPPQYVGFVVLGGAM
                     TAFWLNVIWMMAQQLWWEKSQGNLELYFAAPMHIMAVLFGMALGGFVMSSTRAAAVLV
                     IATTVYGVSFSIDQWALLIGVFLLTLAALYGLGMMLASLFLMWGREAWHLTQLLVEPV
                     YFVSGLNFPIARLGVLGSVAISILPLAVGLDAMRQLAFAGSAYPVGTPSPAIEALILL
                     AMVVVFIVLSRWTLATLERRARSEGRLSVRWQ"
     gene            complement(4959..6116)
                     /locus_tag="VHM48_06050"
     CDS             complement(4959..6116)
                     /locus_tag="VHM48_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755004.1"
                     /translation="MSGTAQPTPAESRPANAQPLAIDVRGIRRVYKVKPKPVVALDGV
                     DLAVEPGEFFGLLGPNGAGKTTLIKILTTLLLPSEGTARIFGFDVERQTKQIRRIMNM
                     VAGGEQSGYGILTVREQLWMFSQFYGLPARDGWNRVDELIDAVGLDEQREQRVSTLST
                     GQRQKMNFARGLLNDPWIFFLDEPTLGLDVAAARSVRELVLAWKAAVPGRTVLLTTHY
                     MAEADELCDRIAIVDRGRILALGTPDELKKRVQRESVFRLELDRLDGGVGALRQLPGV
                     VSAALAIGDQGGDDDRQPVEVNLALVDDGALGGVVGALGGLGAHILALRKSEPSLEDV
                     FVELVGRGFNEDGPAGPDDDRGDHHDRRDRHGPGNAGATDEAREREVEVVG"
     gene            6281..7279
                     /locus_tag="VHM48_06055"
     CDS             6281..7279
                     /locus_tag="VHM48_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755005.1"
                     /translation="MSNGTPSREVSPAYGSLLWTIPVRGAAGQNPFFDDLGTSLYASD
                     GWGRAPAPTLRFRRFDALTGEEQARWPCGSAVRCATLVGDGDLLVATDQRLAQLDATT
                     LVERRRWNRSVKHANSIAVAGSTAIAANWRLPTVTLVDLETGLVRRKRHGEMTAVVGR
                     PGGDPLLVGGASGGIAAIDPASGTIRRLRAAPAALDATLTPDGEALWLTTGIRVVVTE
                     RPDGASLRPGDATTRLERHPLGEGDATEIDVPMPVRTVACGVDGELWLTPGPVQGSEQ
                     FVAIRPNDADDAWRLWRAPTGQLVAALSPAARLVLTSARPPDDLTTTFRCHRLEWG"
     gene            complement(7306..8241)
                     /locus_tag="VHM48_06060"
     CDS             complement(7306..8241)
                     /locus_tag="VHM48_06060"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX2755006.1"
                     /translation="MIGDRLAVGEEVADALAEGRPVVALESTLISHGLPYPQNLDVAR
                     ASEAAVRESGAVPATVAIHDGRLLVGLDVAALEALATAPAGSVLKAARPSLALALASG
                     GWAATTVSATMIAAHAAGIRMFATGGIGGVHRGALAGSGGAAPTLDVSSDLEELGRTP
                     MVVVCAGPKAILDVPATLEYLETHGVPVVTIGQAELPGFYARTSGLASPQSTPDLAGA
                     AAIVRVHLELALGSVLVCVPAPEAEALPGDVARDAIDRAIAEADVAGIRGPATTPWLL
                     ARIAAITEGASIRANTALIVNDARVAGELAVALRA"
     gene            complement(8238..9200)
                     /locus_tag="VHM48_06065"
     CDS             complement(8238..9200)
                     /locus_tag="VHM48_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX2755007.1"
                     /translation="MPPALPARRRSRSAGSRPPRIVVLGDLMVDVVLTPARPLQSGTD
                     VPGRVVMRQGGSAANTARWLARLGARTTLVCAVGRDHEGRELVSALEGDGVRVRATRV
                     AGERTGRIGVLVAPGGERSFVADRAAADGLAPRDLSAAMFQVDLLHLPAYSLLGEPLG
                     LTGRRAIELARAGGALVSLDLSSVGPLLADGRRAAFRLVREAAPDVLFATRVEAEAIV
                     GGRDTTGLGEIARIVVIKHGRSGASVLASATTGRLAFDVATTAIEATDTTGAGDAFDA
                     GFLVAWLSAEAATRRRPAALRRAVLAAHRAAGRQLSTAARELAL"
     gene            9335..11491
                     /gene="tkt"
                     /locus_tag="VHM48_06070"
     CDS             9335..11491
                     /gene="tkt"
                     /locus_tag="VHM48_06070"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2755008.1"
                     /translation="MPTATETLDRLAIDTIRTLSIDGVQQANSGHPGAPMGAAPMAYT
                     LWTRFLRHAPTHPDWPDRDRFVLSAGHASMLLYSLLHLTGYDVSLDDLKAFRQWGSKT
                     PGHPEYGLTPGVEATTGPLGQGFTNAVGMAIAETRLAAEFNRGGHDIVDHWTYTLCSD
                     GDLQEGISSEAASLAGHLRLRKLVALYDDNHIQLDGPTSMAWSEDVVARFEAYGWHAV
                     RVEDGNDVEAIAAAIEEARADDRPSLIAVRTHIGFGSPNRQDTQKAHGAPLGPDEVRL
                     VKEAYGWDPDKTFYEPPEALDLFRRAVPAGDDLVEAWQGAFARYESTEPELAREYRRR
                     VMKREPGNGWDGGLKTYAVGEEVATRNASQDAIQALAATLPELFGGAADLSESNLTDV
                     KGADSFEADDSGRNLRFGVREHAMGGIANGIAYHGGFIPYVGTFLTFSDYMRGSVRLA
                     ALSGLHAIYVWTHDSVGLGEDGPTHQPVEHYAALRAIPNLWFVRPGDANEAAATWALA
                     VERRGDDPSGPVALSLTRQKLPILAGTAEKAREGLRRGGYVLRDPANGRTPEIILIGT
                     GSELQLAYRAAEALEADGIAARVVSLPCWERYELQDSAYRQSVLPPAVKARVAVEAGV
                     SLGWDRWTGDEGAIVALDHFGASAPAGTIFEKFGFTVDRVVGVARDVLAGRVHGPQAT
                     LEPGHLPAPRGGGGHPTLHHGDPGVERTPSSDPGHS"
     gene            11506..11967
                     /locus_tag="VHM48_06075"
     CDS             11506..11967
                     /locus_tag="VHM48_06075"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX2755009.1"
                     /translation="MRVAFAADHAGAALKDELLRRLSAAGFGHEVSDLGGDGSDPQDD
                     YPDFAARLGFAVRDGEADRGILICGSGVGASVAANKIRGVRTAVCHDTYSAHQGVEHD
                     DMNILTLGSRVIGPEPAWECVVAFLGATFSGEARHRRRLDKVLAIEASERG"
     gene            12260..14035
                     /locus_tag="VHM48_06080"
     CDS             12260..14035
                     /locus_tag="VHM48_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755010.1"
                     /translation="MPDDTLSSPALSMPDLALGDGSADYEAAVARARDEQWAERLWDR
                     DATLWSTNERVQAAIADRLGWLDAPAHFADQVAALEGFGEGIRDAGFTTAVVGGMGGS
                     SLAPDVLHRTFGTADGWLDLRVLDSTDPAAVAAAVDDLDPLATLWIVASKSGTTTEPL
                     AFQADAWDRAERALRAHAAPRNEHAGELMVAITDPGRSEEAIPHHDDLREVFLNPPDI
                     GGRYSALTYVGLVPASLIGLDLDALLASALAMAAACHEPDPAANPGVSLGLAIGTLAS
                     AGRDKLTFIADPTIDSFGAWAEQLIAESTGKHGTGIVPVDREPLGDLAAYGNDRVFVR
                     LSLAGDESAGADSFADAAAAAGHPVIRIALADPIDLGAEAFRWEFATAVAGAVLGIDP
                     FDQPNVEEAKDLTRKVLATAEPGEAPPASAPALVSDPAAGLALHGDAALRLTAGDGSL
                     VGELARHLARRKPDAYLALQAFIAPSAAAGAALDRIRTLLRDATRCATTAGFGPRFLH
                     STGQLHKGGTPSGWFLQLTSDHPVDRPIPGWPYTFGRLIDAQAEGDFAAIESHDLPIL
                     RVHLTDPEQGLVALEAALREALGRG"
     gene            14032..14997
                     /gene="gnd"
                     /locus_tag="VHM48_06085"
     CDS             14032..14997
                     /gene="gnd"
                     /locus_tag="VHM48_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX2755011.1"
                     /translation="MRAASCAARTWPRPTTKGGPVAMRIGFVGLGRMGANMVRRLLKD
                     GHEVVAYNRTAEKTKEIEGEGAIGSYSIAELVAKLEKPRAVWIMVPAGDATEAQIEEL
                     MEHLEPGDTIIDGGNTNFHDDQRRHAALAEKGVNYVDAGTSGGIWGLQVGYCLMVGGD
                     EAAVKPLEPVFLSLAPKDGYLHVGGAGAGHYVKMVHNGIEYGLMQAYAEGFEILHASS
                     YPLDLAGIADLWMQGSVVRSWLLELAARAFKANGQDLDHLKGYVADSGEGRWTIQEAI
                     DHDVPAPVITLSLLTRFRSRQDDSYGAKVLAALRNEFGGHAVKTE"
     gene            15322..17031
                     /gene="zwf"
                     /locus_tag="VHM48_06090"
     CDS             15322..17031
                     /gene="zwf"
                     /locus_tag="VHM48_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2755012.1"
                     /translation="MTDTAVPKRRKTSSKPDAPEVGPETAPDKQPHASPRAKDAPRTM
                     KDLRIARQSRRKAAPRADDNPLREGLRLERVPDPSCLVLFGATGDLAHRKVIPALHQL
                     WRTNLLPHDFVVLAIGRRDYDDDTLRAEFRASAEKYSRVLPIDDTAWRAFAARIRYQK
                     LDFANPADYDSLVTVLAGIDKEQNSRGNHLYYLATQPSAFAEIVAQLGRVGLDHELHE
                     GGWRRIVIEKPFGHDLNSAIRLNREVGKVFRESQVYRIDHYLGKETVRNLLVFRFGNG
                     IFEPIWNRRHIDHIQITVAETIGVENRGAFYEETGASRDFLQNHLLQLMSLVAMEPPA
                     TFDADALRDEKVKVIRAIGEMTTDQMRTDVVRGQYGPGWVAAKAVQGYRSEPEVDPVS
                     ETETYIAARFEVDDWRWSGVPFYLRTGKRLPKRATEIAIQFKEVPHRLFRDSGSDPDP
                     NLLAIRIQPDEGIMLRFGAKVPGLGIDVRSVTMDFTYGSAFTVDSPDAYETLILDALL
                     GDASLFTRADEVEGAWARVTPIIDAWMDEGPPQFPNYEAGTWGPEAADGLLAREGRKW
                     RRI"
     gene            17016..18305
                     /locus_tag="VHM48_06095"
     CDS             17016..18305
                     /locus_tag="VHM48_06095"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2755013.1"
                     /translation="MAKNLTTGGAVIDTPSRPNEPILRWKSRAHSIEEVEQELARIWH
                     EANLDLSVDMDGDHVNDRHIGARTSVMNLVVIAARPEVGERCAATIQRLVGRHPSRTT
                     IVQPADPDGPSWLDAQIVAHCIMPRADAPETCAETVHLTCGGESGRHLDAIVAPLLIH
                     DLPVTVWWPDEPSFGSAPVNDLLVMADRLVVDGSGWHGTGLNQLRRMAATADRLPLAI
                     SDFAMVRQSRWREAIAAIFDIPDFLPYLRHLRRIAVTYGVRDEFGAETTNIVKPLYHA
                     AWLASRLGMQVTKPLAPMTAGVARKPPSRGTGGRLTTLANPSAGLAATLRADRTDVAV
                     VIRPVLSTMPAGTTLRVELLADWRGSELRADVTAEQEAVHVRVWQDGVHALERGFNAP
                     RRTDIDLLAEAIESGGRDPVAVGTLALAAQLAGGPLT"
     gene            18302..19090
                     /gene="pgl"
                     /locus_tag="VHM48_06100"
     CDS             18302..19090
                     /gene="pgl"
                     /locus_tag="VHM48_06100"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2755014.1"
                     /translation="MTEPTVVTLAGADACSLAAAERIIEILDVAIDDHGEAHWVTTGG
                     STPAAIYQHLAQTSLRDELDWRKVHLWWTDDRFVPPDHPLSNAKIANDILLSAHRAGE
                     AGTGGVPIPSDQVHPFETNVAIGEGHDQDWCAHRYAEDIQANGPDTNDDGWPVFDLIL
                     LGIGPDGHILSVFPDSAAFDRREWALGVPAPTHVEPHVPRVTLNPAIVPAAAELLVVC
                     HGDGKAEILRDILRGPRDERALPAQLARRPGATWILDREAARLL"
     gene            19087..19623
                     /locus_tag="VHM48_06105"
     CDS             19087..19623
                     /locus_tag="VHM48_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755015.1"
                     /translation="MSAGSDDRAEHGPADDGSTDGWPADRGLVVRRATAADADAIADV
                     YLASFHATYSFPLAHTDDEVRRWIREVVIVSPEAWVAVDDERVVGMLVVGSGALDQLY
                     VAPDRLGTGIGRRLLDTARERSPQGLTLYTFQVNDRARRFYERNGFVTEAFGDGTAND
                     ERQPDVRYAWRPGSVPSR"
     gene            19620..20471
                     /locus_tag="VHM48_06110"
     CDS             19620..20471
                     /locus_tag="VHM48_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2755016.1"
                     /translation="MTAAGSERWRTFQAPDGVPIAVFRSGDGPPLVLVHGATADHTTW
                     RTSGPLLAARHTLHAVDRRGRGASGDGGPDEPYAIEHEFDDLAAVLDTIAAEAGGPID
                     VVGHSYGGRIALGAALRTASLRRLVCYEGAPPVPGRGYQDTDAGTVRRVEALVTAGDR
                     DEALATFMRDIVGMSEPDLAAFRADPIWLRRAAAIDTTIRELRAETSPAGSLEALGAV
                     AQPVLQVLGGASTEPFAAATLALDARLRNGRVVTIAGARHAAHHTHPAEFVAVIEAFL
                     DDPDMAD"
     gene            20477..20737
                     /locus_tag="VHM48_06115"
     CDS             20477..20737
                     /locus_tag="VHM48_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX2755017.1"
                     /translation="MDWLYWIILGFIAGALSGAVVGGRTARGCLPNVVVGVLGAIVGG
                     WLAQQLGFERGPGFIAAVIVAVVGAIVVRVILEAISSDDRRR"
     gene            20869..22038
                     /locus_tag="VHM48_06120"
     CDS             20869..22038
                     /locus_tag="VHM48_06120"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2755018.1"
                     /translation="MSDPISGVAPVAAPEPSAAPASPAPATDERPYSPGLEGVLAGET
                     SLSKVDGANGRLVYRGYRIGDLVERGTYPAVANLLWTGDWDPDARLATGPIPGPVLAI
                     LRALPRTAKPMDALRTAVSAWGATTGPTYPPTVDQARALTAFSPSALAAFVRIRDGKD
                     PIEPDPSLNLVEGFLYQLTGEIPEPATVRALDAYFIVGAEHGFNASTFTARVVTSTNS
                     DIASAVCGAIGTMKGPLHGGAPSEVVDQLSKMGTVEHAEEWLRDALDHHERLMGFGHR
                     VYRAYDPRAAALRKVAEGMEHKPDWLQLAIQVEDMALRLLAEKYPDRPLKTNVEFYAA
                     PVLMGVGLTPDLFPATFSLARHAGWTAHALEQAATNRLIRPDVRYIGPAERDLPA"
     gene            complement(22042..23043)
                     /gene="corA"
                     /locus_tag="VHM48_06125"
     CDS             complement(22042..23043)
                     /gene="corA"
                     /locus_tag="VHM48_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX2755019.1"
                     /translation="MPNLPPLPRPRLRRPHPAGDLDAPGIAEVDDPRVVEASGLRWIH
                     IEDPRLAHREWLEQHYDFHPLDWEDVYSRNQRSKLDVYDDYLFLVMHFPFFEKASGRL
                     LVAEVDLFVGPDYLITLTEHPLPPLQAMFERMESREELRESTFSKGSGYLLYKIVDAN
                     VDAAFPMLRKMGNKLDRLEDDILEGRSGEIVRDISNAKQEIINFRRIVRPQRAVLRDL
                     ERTKQRYLAEEMEIYFDDISDAAERIWDTLENYKEVVEALESTNESVLTHRLNDSFRI
                     LTAASVILLPLTLIASVYGMNVPVPGENEPYSFFVVVALMGLMLAVLVILFRRRGWL"
     gene            complement(23417..24316)
                     /locus_tag="VHM48_06130"
     CDS             complement(23417..24316)
                     /locus_tag="VHM48_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2755020.1"
                     /translation="MAHSHSAAGRHAGALAAAIGIGVVVLALELIAGIAANSLALLAD
                     AGHVFADVSGMALSLVAIRLAAQPPTDGRSFGLYRLEMLAAALNAVVLLGIAVVVGVE
                     AIRRFAAPPDVQPSVVVLVAALALMANLVSLRLLAPGQRESLTMRGAYLEIAGDLMGA
                     FAVLVAGLVILTTGWRPADAVASLVVAVLIVPRTVRLLRDSVDVLLEATPKGVDLSDV
                     ERHVREAPGVIDVHDLHAWTITSGMNVVSAHVVLGPEARPGDILDHLGTCLSADFDIN
                     HSTFQLETPEHVLWEARAEQTRH"
     gene            complement(24402..>24669)
                     /locus_tag="VHM48_06135"
     CDS             complement(24402..>24669)
                     /locus_tag="VHM48_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2755021.1"
                     /translation="PGRAGGMAFSIRPRAQGGLARLSERELDVVRLVVDGRSNDEIGA
                     RLGIGPKTVESHLRRLFERFDLASRTELATRALREGWLEVPPGT"
BASE COUNT         3794 a         8897 c         8625 g         3353 t
ORIGIN      
        1 cgttgatgta ggggtcgagc ttcaggatcg agacccggaa accgcgtgcc ttgagcaggc
       61 ggccgacgct ggcggcggtg atgcccttgc ccagagagga ggtcacgcct ccggtcacaa
      121 agacatactt cgccatgggt caggtcctcc ggggtttttc tcatgctatc ggaacgtgcc
      181 ctccgggaca accctcgcgg gcgcggtcgt catgcgcggt gagccgtgag cgactcccgg
      241 atcactgaaa tggcacgaat ccggcggccc gatcgtggct gcgatcaggt cctggccggc
      301 cgccgtcggg tggccgtggt ccgtgcccaa tagcgagccc gcgtcccggt cgagatcggg
      361 accgttgaac gccggcgcga tgtcgatgca gaggttcacc ggatcagggg cccaatgtcg
      421 cccggacttt caattcccgt tccctgcgag gatgatgcgg gtcgttcgag cgcggcccgg
      481 tacatgactc atgaggtggg cgatggagtt cgcggacgtc gtgatgaagc gccgggcggt
      541 ccgccggttc gaggagggtg gcgtcccgcg cgagaccctc gagcggatcg cccggctcgc
      601 ccagcgcgcc ccgtcgggcg ggttcagcca gggccagcgc ctggtggtcg tgacggatcc
      661 ggagcggcgt cgcgagatcg cccggatctg cggcgaggag tggtacgagg ccgacttcgg
      721 gccgtggatc agcgagtgcg ccgcccagtt cgtcccgtgc gtctccgagc agatctacca
      781 ccgtcgctac caggagcccg acaagctcca ggacgatggc acggagatcg aatggccgat
      841 cccctactgg tgggtcgacg tcggggcgac gatgcagaac atctggctcg cggcggtcaa
      901 cgaggggctc ggctgcggtt tcgtcggcac cgatgagctc gacgccctcc ggacgtcgct
      961 tggccttccg gccgagttca ccccgatcgg ggtcatgccg gtcggcatcc ccgtgccgga
     1021 cgtccggtca cccagcctca agcgcggctg ggtgccgttc gatgagttcg cccgctggga
     1081 acgctggggc tgaccacgac cgattctgcg gatcggcgac acatgtcggt gtcgccgatc
     1141 cgcctggacg acgaccccga cgcggtcctt cggaaggacg gcgagcaacg cgccacaggc
     1201 tggcatgctg cctgcgtggc cgagctcgaa acgttcaacg gagggggcga gccacgatcc
     1261 ggaacggcgc accggcgcgg gtgatcagga cgccggcaac gatcaccagg ccgccgacga
     1321 cctggcccgg ccggatcggc tccgacagga agaggaacgc cagcagcacg gccaacgccg
     1381 ggacgaggaa ctggtaggcc gatgtccggg tcggcccgat caggcggacg ccgttgagga
     1441 cgatgacatt ggataccccg gccgcgagga acccggagta cagcaccgcc ccgaggacgc
     1501 ccggcgtcag ctgcgacgcg tcgacggtcg cgagctggct ggccgcgatc ggcgcgagca
     1561 cgatcgtccc ggcgaccgtc gcccaggccg tcgtccggag cggggagtgg cggcgcagaa
     1621 acggcgcccc gaatgcggtg tagatcgccc agcagatcgc cgccacgagc gtgatcgcct
     1681 cgccgaacag cgagccgccg aggctcaggc ccggaccgct ggcgatcacg gccgccacgc
     1741 cggcgaacga cacgatccct ccgacgagct tgcccggcgt caggacatcg gagcgggcca
     1801 tgacggcaag gaacgcgacc aggaccgggg tcgaagcgat gatcagcgcc gagtcgcccg
     1861 cagcgatcac cttcaggccg gtcgtccaca ggatctggta gacgccgaag ccgagcgcgc
     1921 cgagcgtgcc gatcgcgatc aggtcgcgcc gcggcaggcc gatcgagccc tcccgccagc
     1981 ggagcaggat caggagcgtg gtggaggcga tcgtgaagcg gaggaaggtg aacccgaccg
     2041 gcggcaggac cgcgaccgcc gacttgacga cgatgaagtt gaacgcccag aggaccatga
     2101 cgccgaggac cgccagctcg gcgaggcggt gctcacgcgc gacatgcggg cgagcagaca
     2161 cgtcgaccgg tcgagtggac acgtcgacgc ccgtcggagt tccgacgggc gttcgcggtt
     2221 ccgtggccgt cgcgcccgtc ggcgctccgg cctcaggcac gtcgattcac gcgccttcgc
     2281 gatcgagctc gagcaggtcc tcctccgcct cctcggtgac cttcagccag acgaacagcc
     2341 cggcgaggac gatgagcggg atgacgatga gcgcggcaag ggtgatgccg agggcgacgg
     2401 caacggtctt gagagccttc atggtcgggt cctccgtacg gtcggggtga tggcaatcga
     2461 tcgaggtggg gggcgcccct caggatttcg ccggtttggg catgaccggc gcccggagct
     2521 tcgggccctg ggtcacgagc tgggcgatgt cgtcgcgggt gaactcggcg aggacgtggt
     2581 gcgaccccgc ctgaccgccc aggaacagac gcacgacgaa cgcgccctcc tgctcgaaga
     2641 agaagacgtc gcgaggcttc tgctcgtcga tgtagcggcc gatcacccgg agggccgtct
     2701 cgtagaaccc ggcgtcgcgg acgggttccg gacccggctt ccctcgccgg gcgaccgatt
     2761 cttccatgaa ccgggcgatg tcgtcgtcga ggaaggtcag cgtctccttg gactgcgagc
     2821 ccatcgactc ggaccacgat ccgccggccg ccccggcggt caccacgccc tggacgatga
     2881 acccgtccgg cgcctccacg aggaggatct cgcgcatccc gcgctggtcg atgaaggccc
     2941 cgatcgagcg gaagacctcc tcgaagtcct ggcgcggact gccctcatag atgcgcatcg
     3001 agcctccctc tggcggcggg tctgtcgcgg tgtccggcgc gatccgcggt cgctgtcgat
     3061 caggagccgg tcgtctggtc gaagagcccg cgatccttcg ctcgccggtc gctcgcccgg
     3121 aacacggcca ctgccccgag tgtagccacc gccccggtcg ccagcaacac gaggacgatc
     3181 tcggcggggc tcggcgacac cgcgccggtc agctgggtgt acagggcgcc cggtccgccg
     3241 acggccgaga tcccgccggg gaagagcgcg tggcggatcc cctcgatcca ccaggtgagc
     3301 ggcgtcagga gtccgagggc ctggaccggc gtcggcagga ccgccagggg gaatacgacg
     3361 ccggagacga ggaacagcgc accagccgcg gcttcggggt atgaccatga ctcctgccgg
     3421 gtctgcatgc agatcgcggc caggagcagg ccgatggcga ggatcgaggt gaccccgagg
     3481 atcatcacga ccacgaggag cggccagtcg atccgcccga tgtcgaacgg gacattgagg
     3541 aagacgacgc cgaccgcgag cgtgatcgcg gcacccatcg ccccgacgcc gatccgggcg
     3601 acgccgcggc cgaagatgac cgcgaggaag tcgctcgggc tgacgtagac gtacttgagc
     3661 atccggtagc gctcccggtc gtcgaggatc atccaggcga ggccgctcac gccgctcagc
     3721 acgaacgacc agagtgcgct cccgacgacg acgaacgcgc ggtattcggg tttggcgccg
     3781 ccgctgatca cgtcgagcat gacgacgagg atcagggccg acgcgagcgg cttggcgacc
     3841 gagtagatga agaacaggag cgggtcggtc cagttcgcct ccatgagcca gccgagccgg
     3901 acggcggtca cgaagctgcg ccggacgtcg atccagccgc ggccacgggt ccgggccatg
     3961 tcgaaaccgc cccattccgg ggacgcgagg ccgacggcgt ccggtcggag cgtcggctcg
     4021 acggcggtca tcgagtcact gccagcggac cgacaggcgg ccctcgctgc gggcccgccg
     4081 ctcgagagtg gcgagggtcc agcgcgagag gacgatgaac acgacgacca tcgcgagaag
     4141 gatcagcgcc tcgatcgccg gcgacggcgt cccgaccgga taggccgagc cggcgaacgc
     4201 gagctggcgc atcgcatcga gcccgaccgc gagcggcagg atgctgatcg ccaccgagcc
     4261 gaggacgccg agccgggcga tcgggaagtt caggcccgac acgaagtaga ccggttcgac
     4321 gaggagctgg gtcaggtgcc aggcctcgcg cccccacatc aggaagaggc tcgcgagcat
     4381 catcccgagc ccgtacagcg ctgcgagggt cagcaggaac acgccgatca ggagcgccca
     4441 ctggtcgatc gagaagctga ctccgtagac ggtcgtcgcg atcacgagga ccgcggccgc
     4501 ccgggtcgag ctcatgacga atccgccgag ggccatcccg aagaggacgg ccatgatgtg
     4561 catcggcgcg gcgaagtaga gctcgaggtt gccctggctc ttctcccacc acagctgctg
     4621 ggccatcatc cagatcacgt tcagccagaa cgcggtcatc gccccgccga gcacgacgaa
     4681 cccgacgtac tgcggcggcg cctgcaacgc gcgatagacg aaaacaaacg cggaggtcgc
     4741 gaggaacggc aacaggatct cgaaagcgat ccagctcttc tcgcggaaca agccggtgac
     4801 ccgtggatag gcccgaccca tgaccgtccg gaggtttgtc agcgcgacct ggcgcgtggt
     4861 ccagatggcg gcctcctggg ggctggcggt cgggcggtag acgaaggcgg ttcgacccgg
     4921 agcgtcgatc gttcgacggc cgtcgtcgcg cgccgcggtc atccgacgac ctcgacttcg
     4981 cgctcgcggg cctcgtcggt cgcgcccgcg ttgccgggtc cgtgtcgatc gcggcggtcg
     5041 tgatgatcgc cacggtcgtc gtcgggaccc gccgggccgt cctcgttgaa gccgcgcccg
     5101 acgagctcga cgaagacgtc ctcgagcgac ggctcggact tgcggagggc aaggatgtgg
     5161 gcaccgagcc cgccgagcgc accgacgacc ccgccgaggg ctccgtcgtc gaccagcgcg
     5221 aggttgacct cgaccggctg gcgatcgtca tcgccgccct gatcgccgat cgcgagggcg
     5281 gcgctgacga cgccgggcag ctgccggagg gcgccgacgc cgccgtcgag ccggtcgagc
     5341 tcgaggcgga agacggactc gcgctggacc cgcttcttca gctcgtcggg tgtgccgagg
     5401 gccaggatcc ggccgcggtc gacgatcgcg atccggtcgc acagctcgtc ggcttcggcc
     5461 atgtagtggg tcgtcagcag gaccgtccgc cccgggaccg ccgccttcca ggcgaggacc
     5521 agctcgcgga ccgagcgggc ggcggccacg tccaacccga gcgtcggctc gtcgaggaag
     5581 aagatccacg ggtcgttgag gaggccgcgg gcgaagttca tcttctggcg ctggccggtc
     5641 gatagggtgc tgacccgctg ctcgcgctgc tcgtcgagcc cgaccgcgtc gatcagctcg
     5701 tcgacccggt tccagccgtc gcgcgccggc aggccgtaga actggctgaa catccagagc
     5761 tgctcgcgca ccgtcaggat cccgtagccc gactgctcgc cgccggcgac catgttcatg
     5821 attcggcgga tctgcttggt ctggcgctcg acgtcgaagc cgaagatccg ggccgtgccc
     5881 tccgacggca ggaggagcgt cgtcaggatc ttgatcagcg tcgtcttgcc ggcgccgttc
     5941 gggccgagca ggccgaagaa ctcgcccggc tcgaccgcaa ggtcgacgcc gtcgagggcg
     6001 acgaccggct tcggcttgac cttgtagacg cgtcggatcc cgcggacgtc gatcgccagc
     6061 ggctgggcgt tcgcggggcg cgactcggcg ggcgtgggct gggcggttcc ggacacgggg
     6121 ctccccgggg agacggctgg gtggaccgcg gagtctacgt caggtcgccg gacttgggaa
     6181 gcgcgccgtc gtccagctgt gagatatcca tcgaacacgg gtgccgctcg ccgcgccccg
     6241 ggcgttgacg cccggcgacc cgcaaaccac catcgtcttg atgagcaacg gcacgccgtc
     6301 acgcgaggtg tcccccgcct acggatcgct cctgtggacg atcccggttc gcggcgcggc
     6361 cggccagaac ccgttcttcg acgacctcgg cacgtcgctc tacgcgtcgg acggctgggg
     6421 gcgggctccg gcgccgacac tccgctttcg ccggttcgac gccctgaccg gggaggagca
     6481 ggcccgctgg ccgtgcggct cggccgtccg ctgcgccacg ctcgtcggcg acggcgacct
     6541 gctcgtcgcg acggatcagc ggcttgcgca actggacgcc acgaccctcg tcgagcgccg
     6601 ccgctggaac cgatcggtca agcacgcgaa ctcgatcgcg gtcgcgggct cgaccgccat
     6661 cgctgcgaac tggcggttgc cgacggtcac gctcgtcgac ctggagaccg ggctcgtccg
     6721 gcgcaagcga cacggcgaga tgacggcggt cgtgggacga cccggcggcg atcccctgct
     6781 ggtcggcggc gcaagtggtg gcattgccgc catcgatccg gcgagcggca cgatccggcg
     6841 gttgcgtgcc gccccggcgg cgctcgacgc cacgttgacg ccggacggcg aggccctctg
     6901 gctgacgacc ggcatccggg tcgtcgtcac cgagcgaccc gacggggcga gcctccgtcc
     6961 gggcgacgcg acgacccgtc tggaacggca cccgctgggc gagggtgacg cgaccgagat
     7021 cgacgttccc atgccggttc ggaccgtggc ctgcggcgtc gacggcgaac tctggctcac
     7081 acccggaccg gtccagggat cggagcagtt cgtggcgatt cggccgaacg atgcggacga
     7141 cgcgtggcgg ctctggcggg cgcccacggg gcagctcgtc gcggcgctat ccccggccgc
     7201 ccggctggtg ttgacgtcgg cccgcccgcc ggacgacctg accacgacct tccgctgtca
     7261 ccggctcgag tggggctgac ggtccgcggt ccgacggatc gggggtcagg ctcgcagggc
     7321 gaccgcgagc tcgcctgcga cccgcgcgtc attgacgatc agcgcggtgt tggcgcggat
     7381 ggatgcgccc tcggtgatcg cggcgatccg ggcgaggagc cagggggtgg tcgccgggcc
     7441 gcggatgccg gcaacgtcgg cctcggcgat ggcacggtcg atggcgtcgc gtgccacgtc
     7501 accggggagt gcctcggcct ccggcgccgg cacgcacacg agcacgctgc cgagcgccag
     7561 ctcgagatgc acgcggacga tcgccgccgc gcccgcgaga tcgggcgtcg actgcggtga
     7621 cgcgaggccg gacgtgcgag cgtagaaccc gggcagctcc gcctggccga tcgtcacgac
     7681 cggcacgccg tgcgtctcga ggtactcgag cgtggccggc acgtcgagga tcgccttcgg
     7741 gccggcgcag acgacgacca tcggcgtccg gccgagctcc tcgaggtccg acgagacgtc
     7801 gagcgtcggc gcggcgccac ctgagccggc cagagccccg cggtggaccc cgccgatccc
     7861 gcccgtcgcg aacatccgga tcccggcggc atgggccgcg atcatcgtcg ccgacaccgt
     7921 cgtcgccgcc cagccgccgc tggcgagggc caacgccagg ctcggccggg cggccttgag
     7981 cacggaaccg gccggcgcag tcgcgagtgc ctcgagcgcc gccacatcga gcccgaccag
     8041 gagccggccg tcgtggatcg ccaccgtcgc cggcacggct ccgctctcgc gaaccgcggc
     8101 ctcggacgcc cgtgcgacgt ccagattctg ggggtacggc aacccgtggc tgatcagcgt
     8161 cgactcgagg gcaacgaccg ggcgaccttc ggcgagcgcg tccgccacct cctcgccgac
     8221 ggccagccga tctccgatca cagcgccagc tcccgcgccg ccgtcgacag ctgccgtccc
     8281 gccgcccggt gcgccgccag caccgctcgc cggagcgcgg ccggccggcg acgcgtcgcc
     8341 gcctccgcac tcagccaggc caccaggaac cccgcatcga acgcatcgcc ggcgccggtc
     8401 gtgtcggtcg cctcgatcgc cgtcgtcgcc acgtcgaagg ccagccgccc ggtcgtggcc
     8461 gaggccagga cggacgcgcc ggaccggccg tgcttgatca cgacgatcct ggcgatctcg
     8521 ccgagccccg tcgtgtcccg gccgccgacg atcgcctccg cctcgacgcg tgtcgcgaag
     8581 aggacgtccg gcgctgcctc gcggaccagg cgaaacgccg cccggcgtcc gtcggccagc
     8641 agcggcccga ccgacgacag gtcgaggctg acgagggcgc caccggctcg ggccagctcg
     8701 atggcccgcc ggccggtcag gccgagcggc tcgccgagca gcgaatacgc cggcaggtgg
     8761 aggagatcga cctggaacat cgccgcgctg aggtcgcgcg gggccagacc atcggcggcc
     8821 gctcgatcgg cgacgaacga ccgctcgccg ccgggcgcga ccagcacgcc gatccgcccg
     8881 gtccgctccc cggcgacgcg ggtcgcgcgg acacggacgc cgtcaccctc gagcgcggac
     8941 acgagctcgc gaccctcgtg gtcccgtccg accgcgcaga cgagcgtcgt ccgggcaccc
     9001 agccgggcca gccagcgggc ggtgttcgcg gccgagccac cctggcgcat caccacccga
     9061 ccaggcacat cggtgccgct ctgaagcgga cgagccgggg tgagcacgac gtcgaccatc
     9121 aggtcgccga ggacgacgat ccgcggcggt cgcgagcccg cggagcgcga gcgtcgccgg
     9181 gcgggaagag cgggaggcat ggtgcgagga tagccgcgcc cgaggcggcg cggcagacac
     9241 ggcaggaccg ggtcagcgac ccgcacgcct acaatcaacc ccgacgcagc gacccggaca
     9301 cgacccgatc cggaccaccg ggagcaccac ctccatgccg acagccaccg agaccctcga
     9361 ccgcctcgcg atcgacacga tccggaccct ctcgatcgat ggcgtccagc aggccaactc
     9421 gggccaccca ggcgccccga tgggcgcggc gccgatggcc tacaccctct ggacccggtt
     9481 cctgcgccac gctccgacgc acccggactg gccggaccgc gaccgcttcg tcctcagcgc
     9541 cggtcacgcc tcgatgctcc tctattcgct cctccacctg accggctacg acgtctcgct
     9601 cgacgacctc aaggcgttcc gccagtgggg ctcgaagacg cccggccacc cggaatacgg
     9661 cctgacgccc ggcgtcgagg cgacgaccgg tccgctcggc cagggattca cgaacgcggt
     9721 cgggatggcg atcgccgaga cccgcctcgc ggccgagttc aaccgcggcg gccacgacat
     9781 cgtcgatcac tggacgtaca cgctctgctc cgacggcgac ctccaggagg gcatctcttc
     9841 ggaagccgct tcgctcgccg gtcacctccg cctgcgcaag ctcgtcgccc tctacgacga
     9901 caaccacatc cagctcgacg gcccgacctc gatggcctgg tccgaggatg tcgtcgcccg
     9961 cttcgaggcc tacggctggc acgccgtccg ggtcgaggac ggcaacgatg tcgaggcgat
    10021 cgcggccgcg atcgaggagg cccgggccga cgaccggccg agcctgatcg cggtccggac
    10081 ccatatcggc ttcggcagcc cgaaccgcca ggacacccag aaggcgcacg gcgcgccgct
    10141 cgggcccgac gaggtccggc tcgtcaagga ggcctacggc tgggatcccg acaagacgtt
    10201 ctacgagccg cccgaggcgc tcgatctgtt ccgccgcgcg gtccctgccg gcgacgatct
    10261 cgtcgaggcc tggcagggag ccttcgcccg ttacgagtcg acggagccgg agctggcaag
    10321 ggagtaccgc cgtcgggtga tgaagcgcga acccgggaac ggttgggatg gcggcctcaa
    10381 gacctacgcc gtcggcgagg aggtcgccac ccggaacgcc agccaggacg cgatccaggc
    10441 tttggcagcg acgctcccgg agctgttcgg cggggcggcc gacctgtccg agtcgaatct
    10501 gaccgacgtc aagggcgccg attcgttcga ggccgacgac tccggacgca acctccgctt
    10561 cggcgtccgc gagcatgcga tgggcgggat cgccaacggg atcgcctacc acggcgggtt
    10621 catcccgtac gtcggcacat tcctgacctt cagcgactac atgcgcggct ccgtccggct
    10681 ggctgccctg agcggcctcc acgccatcta cgtctggacg cacgattccg tcgggctggg
    10741 cgaggacggc ccgacccacc agccggtcga gcattacgcc gcgctgcggg cgatcccgaa
    10801 cctgtggttc gtccggcccg gcgacgcaaa cgaggcggcg gcgacctggg ccctcgcggt
    10861 cgaacgacgc ggcgacgatc cgtccgggcc ggtcgcgctg tcgctgaccc gccagaagct
    10921 gccgatcctc gcgggcacgg ccgagaaggc gcgcgaggga ctccgccgcg gcggctacgt
    10981 cctgcgcgac cccgccaacg ggcgcacgcc cgagatcatc ctgatcggga ccggctcgga
    11041 gctccagctg gcctaccgcg ccgccgaggc cctcgaggcc gatggaatcg cggcccgggt
    11101 cgtgtcgctc ccctgctggg aacgatacga gctccaggac tccgcctacc gtcagtccgt
    11161 cctgcctccc gccgtgaagg cccgggtcgc agtcgaggcc ggcgtgtcgc tcggctggga
    11221 ccgctggacc ggcgacgagg gcgcgatcgt cgccctcgac catttcggtg cgtcggcgcc
    11281 ggccggcacc atcttcgaga agttcgggtt cacggtcgat cgcgtcgtcg gcgtcgcccg
    11341 cgacgtgctt gccgggaggg tccacggccc gcaggcgacc ctggagcccg gccatcttcc
    11401 ggcgccgcgc ggcggcggcg gacatccgac cctgcatcac ggcgaccccg gcgtcgagcg
    11461 cacgccgtcg tccgatccgg gccacagctg agccggggcc gaacgatgcg cgtcgccttc
    11521 gccgccgatc atgccggcgc cgccctcaag gacgagctgc tccggcggct gtcggccgcc
    11581 ggcttcggcc acgaggtgag cgacctcggc ggcgacggct ccgacccgca ggacgactac
    11641 cccgacttcg ccgcgcgtct cggcttcgcc gttcgcgacg gggaggccga ccgcggcatc
    11701 ctgatctgcg gctcgggtgt cggcgcgagc gtggcggcca acaagatccg cggggtgcgg
    11761 actgccgtgt gccacgacac gtactcggct catcaggggg tcgagcacga cgacatgaac
    11821 atcctgaccc tcggctcgcg ggtgatcggc cccgaacccg cgtgggaatg cgtcgtcgcc
    11881 ttcctcggcg cgaccttcag cggcgaggcc cgtcaccgcc gccggctcga caaggtcctc
    11941 gcgatcgaag cgtccgagcg cggctgagca cactgacgac ccgccgccgg cgagcgatcg
    12001 ggcccgatgt ttccaaggca ttgcttacga gggcaacgtc gcgaaaagcc gccctgatcg
    12061 acttcagccg gccgaacgat gcgtgaccga catcgaccga cccggaacac gcgtcctgag
    12121 ccgacgtgcc ggcccttcgc gatcaagacc atgcatctca agcatcggcg gcggtcacca
    12181 gccatcagag ctgcgaagcg cgccggttgc gacgatcacg caaggtgcgc ccgaacgcgg
    12241 tcggactacc atggacgcga tgcctgacga caccctctcc agccccgccc tctccatgcc
    12301 cgacctcgct ctcggcgacg gctccgcgga ttacgaagcc gccgtcgccc gcgcccggga
    12361 cgagcaatgg gccgaacggc tctgggatcg cgacgcgact ctgtggtcga ccaacgagcg
    12421 ggttcaggcg gcgatcgccg accgtctcgg ttggctcgac gccccggccc acttcgccga
    12481 ccaggtcgcc gcgctcgagg gattcggtga agggatccgc gatgccgggt tcacgaccgc
    12541 ggtcgtcggg gggatgggcg gcagcagcct cgccccggac gtcctccacc ggaccttcgg
    12601 gaccgccgac ggctggctcg acctccgcgt cctcgactcc accgacccgg cggccgtcgc
    12661 cgcggcggtc gacgatctcg atccgctcgc gaccctctgg atcgtcgcca gcaagtcggg
    12721 cacgaccacc gagccgctcg ccttccaggc cgacgcctgg gaccgcgccg aacgggccct
    12781 tcgcgcccac gccgcgccgc gcaacgagca cgccggcgag ctgatggtcg cgatcaccga
    12841 ccccggccgc agcgaagagg cgattcccca ccacgacgac ctccgcgagg tctttctcaa
    12901 cccgcccgac atcggcggcc gctactccgc gctgacgtac gtcgggctcg ttccggccag
    12961 tctcatcggg ctcgatctcg acgccctgct ggcgtcggcg ctggcgatgg ccgcggcctg
    13021 ccacgagccc gaccccgcgg cgaacccggg cgtgagcctc ggcctcgcga tcgggacctt
    13081 ggcgagcgcc ggccgcgaca agctgacgtt catcgccgac ccgacgatcg acagcttcgg
    13141 ggcatgggcc gagcagttga tcgccgagag cacgggcaag cacgggaccg ggatcgtccc
    13201 ggtcgaccgg gagccccttg gcgacctcgc ggcgtacggc aacgatcgcg tcttcgtccg
    13261 cctgtcgctc gctggtgacg agtccgccgg cgccgattcc ttcgccgacg cggccgccgc
    13321 ggccggtcat ccggtgatcc ggatcgccct cgccgatccg atcgatctcg gcgccgaggc
    13381 cttccgctgg gagttcgcga ccgcggtcgc cggcgccgtc ctcgggatcg acccgttcga
    13441 ccagccgaac gtcgaggagg cgaaggatct cacgaggaag gtcctggcca cggccgaacc
    13501 cggggaggcg ccgcccgcat ccgccccggc gctcgtcagc gacccggccg ccgggctcgc
    13561 cctccacggc gatgccgccc tccggctgac cgccggcgac ggctccctcg tcggcgagct
    13621 ggcccgtcac ctggcccggc gcaagcccga cgcgtacctc gcgctccagg cattcatcgc
    13681 gccgtcggct gccgccggcg cagcgctgga tcgaattcgc accctgctcc gcgatgcgac
    13741 gcggtgcgcc accacggccg ggttcggacc acgtttcctg cactcgaccg gccagctcca
    13801 caagggcggg acgcccagcg gctggttcct ccagctcacg tctgaccacc cggtcgaccg
    13861 gccgatcccg ggctggccgt acacgttcgg ccggctgatc gacgcccagg ccgaaggcga
    13921 cttcgccgcc atcgaatcgc acgacctgcc gatcctgcgc gtccacctca ccgatccgga
    13981 gcaagggctc gtcgctctcg aggcggcact gcgcgaggcg ctcggacggg gctgagggca
    14041 gcatcctgcg cggcccggac gtggccgcgg ccaacaacca agggaggacc agtcgcgatg
    14101 cggatcgggt tcgtcggtct cggtcggatg ggcgccaaca tggtccgccg cctgctcaag
    14161 gatggccacg aggtggtggc ctacaaccgc accgcggaga agaccaagga gatcgagggc
    14221 gagggcgcga tcggctcgta ttcgatcgct gagctcgtcg ccaagctcga gaagccgcgc
    14281 gcagtctgga tcatggtccc ggcgggcgac gcgaccgagg cccagatcga ggagctgatg
    14341 gagcacctcg agccgggcga cacgatcatc gacggcggca acacgaactt ccacgacgac
    14401 cagcgccgcc acgcggccct tgccgagaag ggcgtgaact acgtcgatgc cggcacgtcg
    14461 ggcgggatct ggggcctcca ggtcggctac tgcctgatgg tcggcggcga cgaggcggcc
    14521 gtgaagccgc tcgagccggt cttcctctcg ctcgccccga aggacggcta cctccacgtc
    14581 ggtggagccg gcgccggcca ttacgtgaag atggtccaca acggcatcga gtacggcctg
    14641 atgcaggcct acgccgaggg cttcgagatc ctccacgcct cgtcgtatcc gctcgacctc
    14701 gccgggatcg ccgatctctg gatgcagggc tcggtcgtcc gctcctggct gctggagctc
    14761 gcagcgcgcg cgttcaaggc gaacggccag gacctcgacc acctcaaggg ctacgtcgcc
    14821 gactcgggtg agggccgctg gaccatccag gaagcgatcg accacgacgt cccggccccg
    14881 gtcatcacgc tctcgctgct gacccggttc cgctcccgcc aggacgattc gtacggcgcg
    14941 aaggtgctgg ccgcgctccg gaatgaattc ggcggccatg ccgtgaagac ggaatgaacg
    15001 acatacgacc tgtggaatcg gcacgtgctc accgaggtcc ggggcggagc tcggcggcgc
    15061 catcggcccg aggtgggcat gcggtgaaga ccgagtgaac atgaccgagg ggctcacctc
    15121 gccagatacc accgggtgtg gtgggagtcg ccagccaggc gccgaatcgg gtcgaggcga
    15181 gctgaaaaga ctgaggagtt tgccgtcggc ggctgacagg tccgcagtgg cggctcttga
    15241 gaccactcga ggcttggcgt tcaccacggg ggatggtgga tggcgagacc gggatcggat
    15301 cacggctcgg acggcgaaac gatgaccgac accgcggtcc cgaagcggcg gaagacgtcc
    15361 tcgaagccgg acgcgcccga ggtcggcccc gagaccgcgc cggacaagca gccgcacgca
    15421 tcgccgcgag cgaaggacgc gccacggacg atgaaggacc tccggatcgc ccgccagagc
    15481 aggcgcaagg ctgcgcccag ggccgacgac aacccgctcc gcgagggtct ccgcctcgag
    15541 cgcgtcccgg acccgagctg cctcgtcctg ttcggcgcca ccggcgacct cgcccaccgc
    15601 aaggtcatcc ccgcgctcca ccagctctgg cggacgaacc tgctgccgca cgacttcgtg
    15661 gtcctcgcga tcgggcggcg cgactatgac gacgacacgc tccgggccga attccgggcg
    15721 tcggccgaga agtactcgcg cgtcctgccg atcgacgaca ccgcgtggcg tgcctttgcc
    15781 gcccggatcc gttaccagaa gctcgacttc gcgaacccgg ccgactacga ctccctcgtg
    15841 acggtcctcg cggggatcga caaggagcag aactcgcgcg gcaaccacct ctactacctc
    15901 gcgacccagc cgtcggcctt cgccgagatc gtcgcccagc tgggccgcgt cggcctcgat
    15961 cacgagctcc acgagggcgg ctggcggcgg atcgtcatcg agaagccgtt cggtcacgat
    16021 ctcaactcgg cgattcgcct gaaccgcgag gtcggcaagg tcttccgcga gtcgcaggtc
    16081 tatcggatcg atcactacct cggcaaggag accgtccgca acctcctggt cttccgtttc
    16141 ggcaacggga tcttcgaacc gatctggaac cgccgccaca tcgaccatat ccagatcacc
    16201 gtcgctgaga cgatcggagt cgagaaccgc ggcgcgttct acgaggagac cggcgcgagc
    16261 cgcgatttcc tccagaacca cctcctccag ctgatgagcc tggtcgcgat ggagccgccc
    16321 gcgacgttcg acgccgacgc cctccgcgac gagaaggtca aggtcatccg ggcgatcggc
    16381 gagatgacga ccgaccagat gcggaccgac gtcgtccgcg gtcagtacgg acccggctgg
    16441 gtggcagcca aggcggtcca gggctaccgt tcggagcccg aggtcgatcc cgtgtccgag
    16501 accgagacct acatcgcagc ccgattcgag gtcgacgact ggcgctggtc cggcgtcccc
    16561 ttctacctcc ggaccgggaa acgcctgccg aagcgggcga ccgagatcgc gatccagttc
    16621 aaggaggtcc cccaccgcct gttccgcgat tcgggcagcg atccggaccc gaacctgctc
    16681 gcgatccgga tccagcccga cgaggggatc atgctccgct tcggcgccaa ggtcccgggc
    16741 ctcggcatcg acgttcgctc cgtgacgatg gacttcacct acggctcggc gttcacggtc
    16801 gattcacccg acgcctacga gacgctgatc ctcgatgccc tcctcggcga cgcgtcgctg
    16861 ttcacccggg ccgacgaggt cgagggagcg tgggcccgcg tcaccccgat catcgacgcc
    16921 tggatggacg agggtccgcc gcagttcccc aactacgagg ccggcacctg gggtcccgaa
    16981 gcagccgacg ggctcctcgc ccgcgaggga cggaaatggc gaagaatctg accacgggcg
    17041 gggcggtcat cgacaccccg agccgcccca acgagccgat cctccgctgg aagtcgcgcg
    17101 cccacagcat cgaggaggtc gagcaggagc tggcccggat ctggcacgag gcgaacctcg
    17161 acctgagcgt cgacatggac ggcgaccacg tcaacgaccg ccacatcggc gcccggacca
    17221 gcgtgatgaa cctcgtcgtc atcgccgccc ggccggaggt cggcgaacgc tgcgcggcca
    17281 cgatccagcg gctcgtcggg cgccacccgt cgcggacgac gatcgtccag ccggccgacc
    17341 cggacggccc gtcctggctc gacgcccaga tcgtcgccca ctgcatcatg ccccgggcgg
    17401 atgccccgga gacctgcgcg gagacggttc acctgacctg cggcggcgag tcgggccgcc
    17461 atctcgatgc gatcgtcgcc ccgctcctca tccacgacct gccggtcacg gtctggtggc
    17521 cggacgagcc gtcgttcggt agtgctccgg tcaacgacct cctggtgatg gccgatcgac
    17581 tcgtggtcga tggatccggc tggcacggca cgggcctcaa ccagctgcgc cggatggccg
    17641 cgacagccga ccgcctgccg ctggcgatct cggatttcgc gatggtccgc cagtcgcgct
    17701 ggcgcgaggc gatcgcggcc atcttcgaca ttccggactt cctgccctac ctccgccacc
    17761 tccggcggat cgcggtgacc tacggcgtcc gcgacgagtt cggcgccgag acgacgaaca
    17821 tcgtcaagcc gctgtaccac gccgcctggc tcgcatcgcg actcgggatg caggtcacga
    17881 agcccctcgc gccgatgacc gccggggtgg cgcgcaagcc gccgagccgc ggcaccggcg
    17941 gtcggctcac cacgttggcc aatccgagcg ccggtctcgc cgccaccctc cgcgccgatc
    18001 ggaccgatgt cgcggtcgtg atccggccgg tgctctcgac gatgccggcg ggcacgacgc
    18061 tccgggtcga gctgctcgcc gactggcgcg gctccgagct tcgggccgac gtcaccgccg
    18121 aacaggaggc cgtccacgtc cgggtgtggc aggacggcgt ccatgccctc gagcgtgggt
    18181 tcaatgcgcc gcgccggacc gatatcgacc tgctcgccga ggcgatcgag tcgggcggcc
    18241 gcgatcccgt cgccgtcgga acgctcgccc tcgccgccca gctcgccgga ggtccgctga
    18301 catgaccgag ccgacggtcg tcaccctcgc cggagccgac gcctgctcgc tcgcggcggc
    18361 cgagcggatc atcgagatcc tcgatgtcgc catcgacgac cacggcgagg cgcactgggt
    18421 gacgaccggc ggatcgacgc ccgccgcgat ctatcagcac ctggcccaga cctcactccg
    18481 cgacgagctc gactggcgga aggtccacct gtggtggacg gacgatcgct tcgtcccgcc
    18541 ggaccatccc ctctcgaatg ccaagatcgc caacgacatc ctgctcagcg cccaccgcgc
    18601 cggggaggcg gggaccggcg gtgtcccgat tccgagcgac caggtccacc cgttcgagac
    18661 gaacgtcgcg atcggcgagg gccacgacca ggattggtgc gcccatcgct acgccgagga
    18721 tatccaggcc aacggccccg acacgaacga cgacggctgg cccgtcttcg acctgatcct
    18781 cctcgggatc gggccggatg gtcacattct ctcggtgttc ccggactcgg ccgcgttcga
    18841 tcgtcgcgaa tgggcactcg gcgtcccggc gccgacccac gtcgagccgc acgtgccgcg
    18901 ggtcaccctc aacccggcga tcgttcccgc cgccgccgag ctcctcgtcg tctgccacgg
    18961 cgacggcaag gcggagatcc tccgggacat cctgcgtggt ccgcgtgacg agcgcgccct
    19021 gccggctcag ctcgcccgcc ggccgggcgc cacgtggatt ctcgatcggg aggccgctcg
    19081 gctgctgtga gcgccgggtc cgacgaccgc gccgagcacg ggccggccga cgacgggtcg
    19141 accgacggct ggcctgccga tcgcgggctg gtcgtccgtc gggcgaccgc ggccgatgcc
    19201 gacgcgatcg cggacgtcta cctcgcctcg ttccacgcga cgtactcgtt ccccctggcc
    19261 cacaccgacg acgaggtccg gcgttggatc cgcgaggtcg tgatcgtcag cccggaggcc
    19321 tgggtcgcgg tcgacgacga gcgcgtcgtc gggatgttgg tggtcggctc gggcgccctc
    19381 gaccaactct acgtggcgcc ggatcgactg ggcacgggga tcgggcggcg gctgctcgac
    19441 acggccaggg agcgctcccc gcagggtctg accctgtaca ccttccaggt caacgatcgg
    19501 gcccgccgct tctacgagcg gaacggcttc gtcacggagg cgttcggcga cggcacggcc
    19561 aacgacgagc gccagccgga cgtgcgttac gcctggcggc ccgggtcggt cccgtcgcga
    19621 tgacggcggc gggcagcgag cgctggcgca cgttccaggc gccggacggc gtcccgatcg
    19681 cggtcttccg atccggtgac gggccacccc tcgtgctggt ccacggcgcg acggccgacc
    19741 acacgacatg gcggaccagc ggacccctgc tcgccgcccg ccacacgctc cacgcggtcg
    19801 atcgtcgcgg tcggggcgcg tcgggcgatg gcggcccgga cgagccctat gcgatcgagc
    19861 acgagttcga cgacctggcc gccgtcctcg acacgatcgc cgcggaggcc ggtgggccga
    19921 tcgatgtcgt cgggcactcg tacgggggcc ggatcgcgct cggggccgcc ctacggaccg
    19981 cgagcctgcg ccggctcgtc tgctacgagg gggcgccgcc ggtcccggga cgcggctacc
    20041 aggacacgga cgcgggcacg gtccggcggg tcgaggcgct cgtcaccgcg ggcgaccgcg
    20101 acgaggcctt ggcgacgttc atgcgcgaca tcgtcgggat gagcgagccg gacctggccg
    20161 ccttccgggc cgacccgatc tggttgcgcc gtgccgccgc gatcgacacg acgatccgtg
    20221 agctccgcgc ggagaccagc ccggccggtt cgctcgaggc gctcggcgcg gtcgcccagc
    20281 cggtcctcca ggtcctcggg ggcgcgagca cggagccgtt cgcggcggcg acgctcgccc
    20341 tggacgcgcg gctgcggaac gggcgcgtcg tcacgatcgc cggcgcgcgg cacgcggccc
    20401 accacaccca tccggccgag ttcgtcgccg tgatcgaggc cttcctcgac gatccggaca
    20461 tggcagactg acggccatgg actggcttta ctggatcatc ctcgggttca tcgcgggagc
    20521 gctgtcgggc gcggtcgtcg gcggccggac ggcccgcggc tgcctgccga acgtcgtggt
    20581 cggggtcctc ggcgcgatcg tcggcggctg gctcgcccag cagctcgggt tcgaacgcgg
    20641 tccggggttc atcgccgcgg tgatcgtcgc cgtcgtcggg gcgatcgtcg tccgcgtcat
    20701 cctcgaggcg atcagctcgg acgaccgccg ccgctgatcg ggcgccgccc acgcgcaacc
    20761 ctgacggtgc cgccctcccc gatcccccgc gaaggggccg accggcccgg cgcctaccag
    20821 ccgtccggcc cgtcggcgcg tggcgcacga ggcgactacc atccgacgat gagcgatccc
    20881 atctctggcg tggcgcccgt cgcggcccct gaaccgagcg ccgctcctgc cagcccggct
    20941 cccgcaaccg acgaacggcc gtactcgccg ggcctcgagg gcgtcctcgc cggcgagacc
    21001 tcgctctcca aggtcgacgg cgccaatggc cgcctcgtct atcgcggcta ccggatcggc
    21061 gatctcgtcg agcgcggcac ctaccccgcg gtcgcgaacc tgctctggac cggcgactgg
    21121 gatccggacg cgcggctcgc caccggcccg atcccgggac ccgtcctcgc gatcctccgc
    21181 gccctgcccc ggacggcgaa gccgatggac gcactccgga cggcggtctc ggcctggggc
    21241 gcgacgacgg gcccgaccta tccgccgacc gtcgaccagg cgcgcgcact gaccgccttc
    21301 tcgccgtcgg ccctggcggc gttcgtccgg atccgcgacg gcaaggaccc gatcgagccc
    21361 gatccctcgc tgaacctcgt cgaaggattc ctctaccagc tcacgggcga gatcccggag
    21421 cccgcgaccg tgcgggccct cgacgcctat ttcatcgtcg gtgccgagca tggcttcaac
    21481 gcctcgacgt tcacggcccg ggtcgtgacg tcgacgaact cggacatcgc atcggccgtc
    21541 tgcggcgcca tcgggacgat gaaggggccg ctccacggcg gcgccccgtc cgaggtcgtc
    21601 gaccagctga gcaagatggg caccgtcgag cacgccgagg aatggctgcg cgacgccctc
    21661 gaccaccatg agcggctcat gggcttcggc catcgggtct accgcgccta cgacccgcgg
    21721 gcggccgctc tgcgcaaggt cgccgagggc atggagcaca agcccgactg gctgcagctc
    21781 gcgatccagg tcgaggacat ggcgctgcgc ctcctggccg agaagtaccc ggaccgcccg
    21841 ctgaagacga acgtcgagtt ctacgcggcg ccggtcctga tgggcgtcgg gctcacgccg
    21901 gacctcttcc cggcgacgtt ctcgctcgct cgccacgccg gctggaccgc ccacgccctc
    21961 gagcaggcgg cgaccaaccg cctgatccgt cccgacgtcc gctacatcgg ccccgccgag
    22021 cgcgatctcc ccgcctgatc cttagagcca gccgcggcgc cggaacagga tgacgaggac
    22081 cgcgagcatc aggcccatca acgcgacgac gacgaagaac gagtacggct cgttctcccc
    22141 ggggaccggc acgttcatcc cgtagaccga cgcgatgagg gtcagcggca ggaggatgac
    22201 cgaggccgcg gtcaggatcc ggaacgagtc gttcaggcga tgggtcagga ccgactcgtt
    22261 cgtcgactcg agggcctcga cgacctcctt gtaattctcg agggtgtccc agatccgctc
    22321 ggcggcgtcc gagatgtcgt cgaagtagat ttccatctcc tcggcgagat agcgctgctt
    22381 cgtccgctcg agatcgcgca ggaccgcgcg ctgaggccgg acgatccgcc ggaagttgat
    22441 gatctcctgc ttggcgttcg aaatgtcgcg gacgatctcg cccgatcggc cttcgaggat
    22501 gtcgtcctcg agccggtcga gcttgttgcc catcttgcgc agcatcggga acgcggcgtc
    22561 gacgttggcg tcgacgatct tgtagagcag gtagcccgag cccttcgaga aggtcgactc
    22621 gcggagctcc tcgcgcgatt ccatccgctc gaacatcgcc tggagcggcg gcagcgggtg
    22681 ctcggtgagc gtgatcaggt agtcgggccc gacgaagagg tcgacctcgg cgacgagcag
    22741 ccgacccgac gccttctcga agaacgggaa gtgcatgacg aggaagaggt agtcgtcgta
    22801 gacatcgagc ttcgagcgct ggttgcggga atagacatcc tcccagtcga gcgggtggaa
    22861 gtcgtagtgc tgctcgagcc actcgcggtg ggcgagacgg ggatcctcga tgtggatcca
    22921 gcggaggccg gaggcctcga cgacgcgcgg gtcgtctacc tccgcgatgc cgggcgcgtc
    22981 gagatcaccg gccggatggg gtcggcgaag ccgcgggcgt gggagcggcg gcaggttcgg
    23041 catcgactgg gaggcttcat gatgcgatcg gccgcatcgt agcctgtcgg tccggctgtc
    23101 gtctcgcccc cttgtccgac tccgccaggc gcgccggtcg agatcttgcg tcacccgcac
    23161 gattccgggt cgacgttgga tcccatgcac gcgtcggacc cgccgctcgt ggattcgtcc
    23221 acgctgttgc gccagctgca aggagtggcc ggggcagccg ccatccgcct ccgattgcgc
    23281 agtctcgttg catccgatgc accgaatcga cctgccacgg cccgaagctt cggcgggatc
    23341 agacgacgcg ggcacccgat gcaacgaccg accgggcgtc acgccctcat cggccgcgat
    23401 ggcgcgacgg ccggcgtcag tgtcgcgtct gttcggctcg ggcctcccac aggacgtgtt
    23461 cgggcgtctc gagctggaag gtcgaatggt tgatgtcgaa gtcggccgac aggcacgtcc
    23521 cgaggtggtc gaggatgtcg cccggccttg cctcggggcc gagaacgaca tgtgccgaaa
    23581 ccacgttcat cccgctcgtg atcgtccagg cgtgaagatc gtggacatcg atgaccccgg
    23641 gcgcctcgcg gacgtgccgc tcgacgtcgg agaggtcgac ccccttcggc gtggcctcga
    23701 ggaggacgtc gacgctgtcg cggagaaggc ggaccgtgcg gggcacgatc agaaccgcga
    23761 cgacgagcga cgcgacggca tcggccggcc gccagccggt cgtgaggatc acgagccctg
    23821 cgacgagcac cgcgaacgct cccatgaggt cgccggcgat ctcgagatag gcgccacgca
    23881 tcgtcaggct ctcccgctga ccgggtgcca ggagccggag ggagacaagg ttcgccatga
    23941 gggcaagcgc tgccacgagg acgacgactg acggctggac gtccggcggt gcagcaaacc
    24001 ggcggatggc ctctacgccg acgacgacgg cgatcccgag gaggaccacc gcgttgagcg
    24061 cggccgcaag catttcgagg cggtataacc cgaacgaacg gccgtcggtc gggggctggg
    24121 cggcgagacg gatcgccacg agggacagcg ccatacccga tacgtcggcg aagacgtgac
    24181 cggcatcggc gagaagggcg agcgagttgg ccgcgatccc ggcgatcagc tcgagggcga
    24241 ggacgacgac gccgatcccg atcgctgccg cgagagctcc ggcatggcga ccggccgcag
    24301 agtgcgagtg agccacggac cgagcgtagc gtgttctcgt ccgtgaagcg gcgaacatcg
    24361 tccggattcg tccgacacca gacgtcggtc gtttgcccgg ttcacgtgcc gggcggcacc
    24421 tcgagccagc cctcgcggag ggcgcgcgtc gcgagctcgg tccgggacgc gaggtcgaac
    24481 cgctcgaaca ggcggcgcag gtgcgactcg acggtcttcg ggccgatccc gagccgggcg
    24541 ccgatctcgt cgttcgagcg gccgtcgacg acgagccgga cgacgtcgag ctcgcgctcg
    24601 ctgaggcggg cgaggcctcc ctgggcgcgc gggcggatcg agaacgccat tccgcccgcg
    24661 cgcccaggg
//
LOCUS       DATAIW010000190         7671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_69668, whole genome shotgun sequence.
ACCESSION   DATAIW010000190 DATAIW010000000
VERSION     DATAIW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69668"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2561)
                     /locus_tag="VHM48_06140"
     CDS             complement(<1..2561)
                     /locus_tag="VHM48_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2755022.1"
                     /translation="MRRPDAWYVGEWSRLAKAVFGADVDADLAVDPARARDAVRQALR
                     QRAQAEFGAAIGRGETLERALARSVSDLATLPEYHAAWAMAEGIGRVPEGAEASAIGH
                     AVLLHRRRQLHRAWTIVRELSDEALARSIPVEAVDAALAAGTAEGRRRALAIATSATG
                     MGAAVLVDLAGRLLAFEERRAAAELVTALRRRPPGDLDERRRHALTLIERWLEPSAMS
                     VPAGAVPIGVIGYGSPDPVLTSGNLGDSIQTLALVGNLVRLSNVTFTGDAGLGELATE
                     LQGRIPAELRIPDVAGAVHLVGVDRDFSSADAIPPGTWLVAFGWHMHPLFDLRYDFPY
                     HTDIRPVFLSFHVNRLDMLSDEAREYLRRHGPIGCRDWSTVFLLLSAGIDAFFSGCLT
                     MTIDGLYPSREAAYAGSGMVGIIDLPPDAAGRDAQDVRVYSHQSDAIRSMSLTEGLRA
                     ADAAMAAYQRDLARAVTGRLHAYLPLTSVGVPVEFRTGSPGDVRFAGLTGFQPGDARL
                     AELRDGIRGLLAPILAKVVGGADEAEVYATWRDLTRDRVAEARARFQAPIEDPPTTID
                     IDAAVATSMAASRRFGPHEEVDPATITDVVLSFDQNLTLSAAVLVESIVANASGPVRL
                     WVLARGLSGAYQEWLAGAFPALPMTFVPCDGISYGPRGRPRRVPARITVSTMDRLLLP
                     AMLEDVARVVYLDVDMLMLDDICRLAGMDLGGRPVGARDSTVSEASEWQRAGRALAEP
                     LASELRRRMGVRHGYGNPALNAGLLVMDLDRMRRDGFTPTSLGLVERYGLHDQDTMLA
                     YVGPDRAVIEPRWNAMPVLEDVRDPSLIHWASFGKPWEPELTYGQDRWLEYAARL"
     gene            complement(2908..3696)
                     /gene="radC"
                     /locus_tag="VHM48_06145"
     CDS             complement(2908..3696)
                     /gene="radC"
                     /locus_tag="VHM48_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX2755023.1"
                     /translation="MDEGWSAVDAAPDAREARHDRPRGRAIRELPASERPRERLAMRG
                     AAGLSAAELIGLLWGSGSRGRSAVDLAEDALARHDGLTGLARAGELELEALPGIGVAK
                     AAQLTAAFELGRRLMADWPTGRWTIRAPHDVADRLMLQMGRLEREELRVVLLNTKNVV
                     LRVTTVYQGNVSSSLVRVGELFRDAVRLNASGVILVHNHPSGDPTPSPDDLHLTAEAL
                     AAGRLLDIDLLDHLVVGHDAYVSLRDRGVSFDRPGRGRMAGEAS"
     gene            complement(3933..5012)
                     /locus_tag="VHM48_06150"
     CDS             complement(3933..5012)
                     /locus_tag="VHM48_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2755024.1"
                     /translation="MSEKPPANCGEPEPGEAEPGEAAPGVDVDVDADSGPEEPPPPSR
                     PGLTTFTIEGRAAPGLFVVGWLASISGLALVLIGAVSPSALFLYFLGPVVLTIGLVAG
                     AGSQAIERRARGSAYAGPSPYLVFATIVAAVFAVGAVVGLGLRLVLGSTEVPGYLVNL
                     VGVAIQATVFLGIIRLTVVGTEALTWTDMGWRRLDTNAIRNLLFGAALAIPVIGLTAV
                     VADALVAIFNQVPPSPLPATGLATGLVVQLIAGAVIAPIAEETVFRGFAISAWQRTVG
                     ERGALVRASVVFALAHVITVSGDSLAQVGGLIAVGFATRIPVAFALGWLYLRTRSIWA
                     PLGLHMAFNGILLILAEYVRMNGAA"
     gene            complement(5058..5753)
                     /locus_tag="VHM48_06155"
     CDS             complement(5058..5753)
                     /locus_tag="VHM48_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2755025.1"
                     /translation="MPDILAGIGSAVAGFFADPVVKFVVYSILAYIVVLWLASAYWVF
                     RDMQQRTENAILPYLAASLVIVFLPVFPAAIFVYKIIRPHEKIGEVYERNLAEEALLA
                     EVESIKSCPTCARRVNDEWIICPTCRTRLNRVCPNCSRLVGLDWSLCAWCGKDFERPA
                     TAIASSAGRPTAISSLPGGEETGRIARGAPLEVEAEVESDRPAMSRSMPQTRTTSPRG
                     SSPRSAPDVLPEG"
     gene            complement(5887..7011)
                     /locus_tag="VHM48_06160"
     CDS             complement(5887..7011)
                     /locus_tag="VHM48_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755026.1"
                     /translation="MATKTVVCPECGSAAAPGRYACTECGALLAAVALTPRGGAPTAE
                     PEPEIGIDDGDGPPAADASGDDERGPAAILAATTDVDVADDLPASGGAIVAADAPAEP
                     NVHHPGGDLEFDENAPLAAAAHSMTAPDVLHDLADEPPMEDDASLATAPPTTAPSWPP
                     PGDRGPIARPDQRTPAGTYLPPSAVLPPLDAGFAGGGAGGSPAPGGAGMPPVPGVATR
                     SSDQASPASSWAGSGSAALSDALGSARVTAAASRRAIAAGAGLAALGMLLPWVNTLPG
                     ASPFAGYLDRWGLAGPGLWLVFLGLVVLTAVAVSSGRAASWPIGLPAVATAAFLVGLV
                     WPYAVGGFGRSIGIWVIVVGAIVLGGGGLLDRHSEAEPTV"
     gene            complement(7055..>7671)
                     /locus_tag="VHM48_06165"
     CDS             complement(7055..>7671)
                     /locus_tag="VHM48_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755027.1"
                     /translation="SVQIFGVAYRELVNPTDASPIVLRVIAGASGPVAMIVVAWILGE
                     LVGGMAVRRIVLGGEPVLGAVVRSAADLVRRPGGAVLAPLVTMAVLVLDLAAVLTVVM
                     IVWTEVRTRLIHPVAEPLATGLALATLGAAWCLALLVTGLIDAWRSAAMTFEMERRAA
                     ASTARSVAGGDEGSHPGPSGGGTIGASAHRRPGGWSADDRGGSL"
BASE COUNT         1158 a         2757 c         2813 g          943 t
ORIGIN      
        1 tgcagtcgcg cggcgtactc gagccagcgg tcctggccgt aggtcagctc cggctcccag
       61 ggcttgccaa agctcgccca gtggatgagg gacgggtcgc ggacatcctc gaggaccggc
      121 atcgcgttcc agcgcggctc gatgacagcc cggtccggcc cgacgtacgc gagcatcgtg
      181 tcctggtcgt gcagcccgta ccgttcgacc aggccgagag aggtcggggt gaagccgtcg
      241 cgccgcattc gatccaggtc catcaccagc aggcccgcat tcagggcagg gtttccatac
      301 ccgtggcgca cgcccatccg tcgtcgcagc tcgctcgcca gcggctccgc gagcgcccga
      361 ccggcgcgct gccactcgct ggcctcgctc accgtggagt cgcgggcgcc gaccggccga
      421 ccgccgaggt ccatcccggc gagccggcag atgtcgtcga gcatgagcat gtcgacatcg
      481 agatagacga cccgggccac atcctcgagc atcgccggca ggagcagccg gtccatcgtc
      541 gagacggtga tccgcgccgg cacccgacgc ggtcggccgc gcgggccgta gctgatgccg
      601 tcgcatggca cgaacgtcat cggcagggcc gggaatgcgc cggccagcca ctcctggtac
      661 gcgccgctga ggccgcgggc gaggacccac aggcggaccg ggcctgaggc gttggcgacg
      721 atcgactcga cgagcaccgc ggccgacagc gtgaggttct ggtcgaagct caggacgacg
      781 tcggtgatcg tggctggatc cacctcctcg tgcgggccga agcggcggct ggctgccatg
      841 ctggtggcga cggccgcgtc gatgtcgatg gtcgtgggcg gatcctcgat gggcgcctgg
      901 aaccgggccc tggcttcggc gacccgatcg cgggtgaggt cgcgccaggt ggcgtagacc
      961 tccgcctcat cggctccgcc gacgaccttg gcgaggatcg gggcgaggag accgcggata
     1021 ccgtcgcgga gctccgccag gcgggcgtcg cccggctgga agcccgtcag gccggcgaac
     1081 cgcacgtcac cggggctgcc ggtccggaac tcgaccggca cgcccacgga cgtcagcggg
     1141 aggtaggcgt gcagccgccc cgtcaccgcg cgggccagat cccgctggta cgcggccatg
     1201 gcggcgtccg cggcgcggag cccctcggtc agggacatgg accggatcgc gtcggactga
     1261 tggctgtaga cgcgcacatc ctgcgcgtcg cggccggccg cgtcgggcgg caggtcgatg
     1321 atcccgacca tgccgctgcc ggcatacgcc gcctcgcggg acgggtagag cccatcgatc
     1381 gtcatcgtca ggcagcccga gaagaacgcg tcgatcccgg cgctgaggag caggaacacc
     1441 gtgctccagt cgcggcaacc gatgggcccg tgccggcgca ggtattcccg ggcctcgtcg
     1501 ctgagcatgt cgagccggtt cacatggaag gacaggaaca cgggccggat gtcggtgtgg
     1561 taagggaagt cgtagcgcag gtcgaagagc gggtgcatat gccagccgaa cgcgaccagc
     1621 cacgtgcccg gcgggatcgc gtcggcgctg ctgaaatccc ggtcgacccc gacgagatgg
     1681 acggcaccgg cgacgtccgg gattcgcagc tccgccggta tgcgtccctg cagctcggtt
     1741 gccagctcgc cgaggccagc atcgccagtg aaggtcacgt tgctcagtcg gacgaggttc
     1801 ccgaccagcg ccagcgtctg gatggagtcg ccgaggttgc ccgaggtcag gacgggatcc
     1861 ggcgatccat agccgatcac cccgatcggg acggcgccgg caggaaccga catggccgac
     1921 ggctcgagcc agcgttcgat cagggtcagt gcgtggcgtc ggcgctcgtc caggtcgccg
     1981 ggaggacggc ggcgcaacgc ggtcacgagc tcggcggctg cccggcgttc ctcgaacgcg
     2041 aggagcctgc cggccaggtc gacgagcacc gccgcgccca tcccggttgc cgaggtcgcg
     2101 atggcgagcg cccgccggcg gccctcggcg gtcccggcgg ccagggcggc gtcgacggcc
     2161 tcgaccggga tggatcgagc gagcgcctcg tcgctgagct cccggacgat cgtccacgcg
     2221 cgatggagct ggcggcggcg gtggaggagg acggcatggc cgatcgccga cgcctccgcc
     2281 ccctccggca cccgtccgat gccctcggcc atggcccacg cggcgtggta ttcggggagc
     2341 gttgccaggt cggagacact tcgggccagc gcccgctcca gggtctcgcc tcgcccgatc
     2401 gccgcgccga attcggcctg ggcccgctgc cgcagggcct gcctgaccgc atcgcgcgca
     2461 cgggcggggt ccaccgccag gtccgcgtcg acgtccgccc cgaagacggc cttcgcgagc
     2521 cgggaccatt ccccgacgta ccaggcatcc ggccgtctca tggcgctcgc cgaggtcctc
     2581 acggcaccgg cagctcgctg acgcgccgtc gatggcggtc gacctgccac atggcggatc
     2641 acggacgtca cacgtcgccg cacccggcgc agggcgtcgg accgggtctc ggtgcgccgg
     2701 aggtcggcga gcaggcggcg gatccgggcg ctccgcgtgg cccgtgcggg cttggtcacc
     2761 gtcgggtcgc cggagccgct ggaggctgca cgacgcgcga tcccgaccga tggagcactg
     2821 gccacctctg cctcggccgc ggcctcccga cggctcggcg ggaagtgtag ccgccaaacc
     2881 cctcgcccac ccggaagggg cccctgctca gctcgcctcg ccggccatgc gcccgcgccc
     2941 gggtcggtcg aacgagacgc cgcggtcgcg caacgagacg taggcgtcgt ggccgacgac
     3001 caggtggtcg aggaggtcga tgtcgagcag gcgaccggcg gccagcgcct cggcggtcag
     3061 gtggagatcg tccggcgacg gcgtgggatc tccgctcgga tggttgtgaa ccaggatcac
     3121 gccgctcgcg ttgagccgga ccgcgtcccg gaacagctcg ccgacgcgga ccagcgacga
     3181 cgacacgttg ccctggtaga cggtcgtgac gcgcaggacg acgttcttgg tgttgagaag
     3241 gacgacccgc agctcctccc gctcgagccg gcccatctgg agcatcagcc ggtcggcgac
     3301 gtcgtgcggc gcgcggatcg tccagcgacc ggtcggccag tcggccatga gccgccgccc
     3361 gagctcgaac gcggcggtga gctgggcggc cttcgcgacg ccgatcccgg gcagggcctc
     3421 cagctcgagc tcgcccgcgc gagccagccc ggtcaggccg tcatgccggg cgagggcgtc
     3481 ctccgcaaga tcgaccgccg agcggccgcg cgagccggaa ccccacagca gcccgatcag
     3541 ctcggccgcc gacagccccg ccgcgccgcg catcgccagc cgctcgcgcg gccgctcgga
     3601 ggccggcagt tcgcggatcg cccggccacg gggccgatcg tggcgcgcct cgcgcgcgtc
     3661 cggcgcggca tcgaccgcgc tccacccttc gtccatcgcc tgcctcgctg ctgcgcggcg
     3721 gtcaggagtc cccggacccg tacgacgaaa gcccgccgat tccttccgcc atccggatcc
     3781 gggaacacga cgaaccctag cgggcggcgc tcacccgccg atcaaccggg cctgcaccgt
     3841 cggttcgccg ccacgcgact gcgttcgtcg cgccggggcg aggaccacgg gcggcaacgc
     3901 cgccatcgtc gttccagcgc gacagcccgc gctcaggccg cgccattcat ccggacgtac
     3961 tcggcgagga tgaggaggat cccgttgaag gccatatgaa ggccgagcgg cgcccagatc
     4021 gaacgggtcc gcaggtacaa ccagccgagc gcgaaggcca ccgggatccg ggtcgcgaag
     4081 ccgacggcga tcagtccccc gacctgcgcg aggctatcgc cgctcacggt gatgacgtgg
     4141 gccagggcga acacgaccga ggcgcggacg agtgcgccgc gctcgccgac cgtccgctgc
     4201 caggccgaga tcgcgaagcc gcggaagacg gtttcctccg cgatcggggc gatgacggca
     4261 ccggcgatca gctggaccac gaggccggtg gcgagtccgg tcgccggcaa cggcgagggc
     4321 gggacctggt tgaagattgc gaccagcgcg tcggccacga ccgcagtgag tccgatcacc
     4381 gggatggcca gcgcggcacc gaacagcagg ttgcggatgg cattggtgtc gagccgccgc
     4441 cagcccatgt ccgtccaggt caatgcctcg gttccgacga ccgtcaggcg gatgatgccg
     4501 aggaagacag tggcctggat cgcgactccg accaggttga cgagatagcc cggcacctcc
     4561 gtcgatccga ggaccaatcg aaggccgagt cccacgacgg caccgacggc aaagaccgcg
     4621 gcgacgatcg ttgcgaacac caggtacggg gatgggccgg cgtacgccga cccgcgcgcg
     4681 cgacgctcga tcgcctggct gcccgcgccc gccacgagcc cgatcgtcag caccacgggc
     4741 ccgaggaagt agaggaacag cgccgacggc gagaccgcgc cgatcaggac gagggcaagc
     4801 ccggagatcg atgcgagcca gccgaccacg aagagaccgg gcgcggcccg gccctcgatc
     4861 gtgaaggtgg tgaggcccgg ccgcgagggc ggcggcggct cctcggggcc ggagtcggcg
     4921 tcgacatcga catcgacgcc gggcgcggcc tcgccgggct cggcttcgcc gggctcgggt
     4981 tcgccgcagt tcgcgggggg cttctcagac atggatccgg cgtccgggaa atcgtcgtcg
     5041 acgcccgcct gcgttcctca accttcgggc aggacgtccg gtgcggaccg cggcgacgag
     5101 cctcgcggcg aggtcgtccg ggtttgcggc atcgagcggg acatggccgg ccgatccgac
     5161 tcgacctcgg cctcgacctc gagcggcgcg ccccgggcga tccgacccgt ctcctcgcca
     5221 ccggggagcg acgagatcgc cgtcggccgg ccggccgacg aggcgatggc cgtcgccggg
     5281 cgctcgaagt ccttgccgca ccaggcgcac agcgaccagt cgagcccgac gaggcgactg
     5341 cagttcgggc agacccggtt caggcgcgtc cggcacgtcg gacagatgat ccattcgtcg
     5401 ttcacccgcc gggcgcaggt cggacaactc ttgatcgatt cgacctcggc gaggagcgcc
     5461 tcctcggcca ggttccgctc gtacacctcg ccgatcttct cgtgcggccg gatgatcttg
     5521 tagacgaaga tcgcggccgg gaagaccggc aggaacacga tcacgaggct ggccgcgaga
     5581 tacggcagga tcgcgttctc ggtccgctgc tgcatgtcgc ggaagaccca gtaggcactg
     5641 gccagccaca ggacgacaat gtaggcgagg atcgagtaga cgacgaactt gacgaccggg
     5701 tcggcgaaga agcccgcaac tgccgaaccg attcccgcga gaatgtctgg catgcggctg
     5761 ggttgcccgc ctccgttgcg ttcgcggctg gtcgatggct gccggcggac cgcgtcccgc
     5821 ccggcgaggc ggccgacgtc cggcgcagtg tagcaggggt ccgatcggcg acccgtccgt
     5881 ggacccctag acggtcggtt ccgcttcgga gtgacgatcg agcaggccgc cgccgccgag
     5941 cacgatggcg ccgacgacga tgacccagat cccgatcgag cgaccgaacc cgccgacggc
     6001 gtagggccag acgagaccga cgagaaacgc ggccgtcgca acggccggca gcccgatcgg
     6061 ccaggatgcc gcccgccccg acgaaacggc gaccgcggtc aggacgacca ggccgaggaa
     6121 gacgagccag aggcccgggc cggccaggcc ccagcgatcg aggtagccgg caaatgggct
     6181 cgcgccgggc agcgtgttga cccatggcag gagcatgccg agcgcggcca gcccggcgcc
     6241 ggcggcgatc gcgcgacgcg aggccgcggc ggtgacccga gcggacccga gggcgtccga
     6301 gagcgcggcc gagccgctgc cggcccagga cgacgccggg gaggcctggt cggaggagcg
     6361 cgtggcgacg cctggaacgg gcggcatccc cgctccacct ggggccggcg aaccgcccgc
     6421 gccgccgccc gcgaatccgg cgtcgagcgg cgggaggacg gccgacggcg gcaggtacgt
     6481 acccgcgggc gtccgctggt cgggcctggc gatgggcccg cggtcgcccg gtggcggcca
     6541 ggacggggcg gttgtcggcg gggcggtcgc cagggaagcg tcgtcctcca tcggcggctc
     6601 gtccgcgagg tcgtggagaa cgtcgggtgc ggtcatcgaa tgggctgccg cggcgagggg
     6661 ggcgttctcg tcgaactcga ggtcgccacc cgggtggtgg acgttgggct cggcgggtgc
     6721 gtcggccgcc acgatggcgc caccagaggc cggcaggtca tcggccacgt cgacatcggt
     6781 cgtcgccgcc aggatggccg ccgggcctcg ctcatcgtcg ccagatgcgt ccgccgcggg
     6841 cggcccgtca ccgtcgtcga tcccgatctc gggctcgggc tccgccgtgg gcgctccgcc
     6901 gcgaggcgtc agcgcgacgg cggcaagcag ggcgccgcac tccgtgcagg cgtaccggcc
     6961 gggagcggcc gccgacccgc actccgggca gacgaccgtt ttcgtcgcca tgaccacctc
     7021 gctctgacct ccggccgacg ttcgccgacc ggtcctacag gctgccacct cgatcgtcag
     7081 ccgaccaacc ccccggacgg cgatgtgcgg atgccccgat tgtccctccg ccggacggcc
     7141 cgggatggga cccttcgtcg cccccggcga ccgatcgggc cgtcgaggct gcggcccgtc
     7201 gctccatctc gaacgtcatc gccgcgctgc gccaggcatc gatcaggccc gtgacgagga
     7261 gtgccaggca ccaggcagcg ccaagtgtcg cgagcgcgag cccggtcgcg agcggctcgg
     7321 cgaccgggtg gatgagtctc gtccgaacct ccgtccagac gatcatcacg acggtcagga
     7381 ccgcggcgag gtccaggacg aggaccgcca tcgtgacgag cggcgccaac acggctccgc
     7441 ccggccggcg gacgagatcg gccgccgagc gtacgaccgc acccaggacg ggctcgcccc
     7501 cgaggacgat ccgccgcacg gccatgccac cgacgagttc gccgaggatc cacgccacga
     7561 cgatcatcgc cacgggaccc gaggcgccgg cgatgacgcg gagcacgatc gggctggcgt
     7621 cggtcgggtt gaccagctcg cggtacgcga caccgaagat ctggacggaa c
//
LOCUS       DATAIW010000191         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_159069, whole genome shotgun sequence.
ACCESSION   DATAIW010000191 DATAIW010000000
VERSION     DATAIW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159069"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHM48_06170"
     CDS             <1..408
                     /locus_tag="VHM48_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755028.1"
                     /translation="VMPDIEYAFLADSAETQPGQKFHVLGGGVSRIGGRTFPLRHPHL
                     ALVLGLLVTAPEVDREHEVRFVLLDPDGGEVAGATGNLQAHGHTDGRDTVLTFSIDFW
                     NLTFAAPGDYSFRILVNGSERKRLPLLVSAPDA"
     gene            401..787
                     /locus_tag="VHM48_06175"
     CDS             401..787
                     /locus_tag="VHM48_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755029.1"
                     /translation="MPDRETRPRGIGHHDTGSRSTLDRRGPARHELPAVDEAGLFAGR
                     QADRIVAAAEAAGATRWLRYFEAMPDRLRDDEPRSLRQVALRARAAYGPKDSVRDALP
                     ADVTEPFLESIDRLIKQLNRRDAGAA"
     gene            complement(820..1407)
                     /locus_tag="VHM48_06180"
     CDS             complement(820..1407)
                     /locus_tag="VHM48_06180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2755030.1"
                     /translation="MPERTASPAFPPDGRVPILRGELVYLRAGERSDIPMFMRWFNDL
                     RTTRTLLFIGPMGTAAEEAWFTQMSEHHGKDRWFFVICRLEDDRPVGSTDLHEVDLRN
                     GNASLGIAIGDPADTGKGYGSDALRSLLGFAFDQLRLERIELDVYDFNDRARGLYERV
                     GFRHEGTLRHALFSDGAFHDVHRMSILRGEWAARP"
     gene            1637..>1826
                     /locus_tag="VHM48_06185"
     CDS             1637..>1826
                     /locus_tag="VHM48_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755031.1"
                     /translation="MVGNRRSSSSGRAAVLALAFALVASAVGMTGPAGPALVTAAGPP
                     RTPGPAHLRPEPGKKGHIN"
BASE COUNT          255 a          671 c          629 g          271 t
ORIGIN      
        1 gtcatgccgg acatcgaata cgccttcctc gccgactccg ccgagaccca gccgggccag
       61 aagttccacg tcctcggcgg cggcgtgtcg cggatcggcg gccggacgtt cccgcttcgc
      121 cacccgcacc tggccctcgt cctcggtctg ctggtcaccg cgccggaggt cgaccgcgag
      181 cacgaggtcc gcttcgtcct cctcgacccc gacggcgggg aggtcgcggg cgcgaccggc
      241 aacctccagg cccacggcca cacggacggt cgggacacgg tcctgacgtt ctcgatcgac
      301 ttctggaacc tgaccttcgc cgcacccggt gactactcgt tccggatcct cgtcaacggc
      361 tcggagcgga agcgcctgcc gctcctcgtg tccgcgcccg atgcctgacc gggagacccg
      421 cccccgcggg atcgggcacc acgacacggg ctcgcgttcg accctcgatc ggcggggacc
      481 ggcccgccac gagctgccgg ccgtcgacga ggcagggctg ttcgcgggtc gccaggccga
      541 tcggatcgtg gccgccgccg aggcggccgg cgcaacccgc tggctccgct atttcgaggc
      601 gatgccggac cgcctccggg acgatgagcc tcgttcgctc cgccaggtgg cgctgcgcgc
      661 cagggccgcc tatggcccga aggattctgt ccgcgacgcg cttccggccg acgtgaccga
      721 gcccttcctc gagtcgatcg accggctcat caagcagctc aaccggcgcg acgcgggcgc
      781 cgcctgagct cgatcaaccg gtcgccgccg gaggcggcgt cacggccgtg cggcccactc
      841 gccgcgaagg atcgacatcc gatggacgtc gtggaaggcg ccatcgctga acagcgcgtg
      901 acgcagggtg ccctcgtggc ggaacccgac ccgttcgtac aggccccgcg cgcgatcgtt
      961 gaagtcgtag acgtccagct cgatccgctc cagccgcagc tggtcgaagg cgaaaccgag
     1021 caggctgcgg agagcgtcgg aaccgtagcc cttgcccgtg tcggcgggat cgccgatcgc
     1081 gatcccgagg ctggcattcc cgttccggag gtcgacctcg tggagatccg tcgacccgac
     1141 ggggcggtca tcctcgagcc ggcagatgac gaagaaccag cggtccttgc catggtgttc
     1201 ggacatctga gtgaaccacg cttcctcagc cgcagtcccc atcgggccga tgaagaggag
     1261 cgtccgggtc gttcgcagat cgttgaacca gcgcatgaac atcgggatgt cggagcgctc
     1321 gccggctcgg aggtagacca gctcgccgcg caggatcggc acgcgaccgt cgggcgggaa
     1381 cgccgggctc gcggtccgtt cgggcatggc ctccagtcta gcggccgctc cctcgctcgc
     1441 gacgctcgcg ctcctcttct ggtgacgccg tcgtcgtcaa gccattgacg cggagcgtgg
     1501 accgcggtct atagtcgcgg acaacgtcgg acgcgcgcct tccgtcccag tcgcccaaga
     1561 ccgcgccagc cggcatcgga ggggttcagc cggttgcgcc gggggcgagt tgcagcctgg
     1621 gtagggaggc tcgctggtgg tggggaaccg tcggtcgtct tcgtcgggcc gggccgcggt
     1681 gctcgctctg gccttcgccc tcgtcgcctc ggcggtcgga atgaccggac cggccgggcc
     1741 tgcgctggtc acggccgctg gcccgcccag gacgccgggc cccgcccatc tccgccccga
     1801 gcccggcaag aaggggcaca tcaacc
//
LOCUS       DATAIW010000192         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_69787, whole genome shotgun sequence.
ACCESSION   DATAIW010000192 DATAIW010000000
VERSION     DATAIW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69787"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHM48_06190"
     CDS             <1..295
                     /locus_tag="VHM48_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755032.1"
                     /translation="VVRESELYGYQIAQRIRERSDGAFVPSEGSLYPTLHRLEADGAL
                     TATWREGERGPRRRWYRITPSGVATLAAHEAEWEAFSDAVTRVASVAREPSGA"
     gene            288..1490
                     /locus_tag="VHM48_06195"
     CDS             288..1490
                     /locus_tag="VHM48_06195"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX2755033.1"
                     /translation="MPDPGERDAFLASVRSRLDLPDAVARDILEELSGHLEDAAAELR
                     AAGLSADDAERRAIGRLGDARALGTELARARRGRRQLLAAVGGGIRAVLVEGIRTWVF
                     LAVLASLTAILALPAASIVRLLLGLRSTSSMLVGMPGSLATVGAVVGGFAYLGWVLPA
                     RFAGPAVRSVRGVRRAVALVGLAVGSAILWFLVPVAMDPVLVVGLPLGPVAFALTALR
                     APERPTIRVGFVPAVIGAGILFLPMTLLALAATDPPLDNWMADISPIGDVPAATDIEN
                     TALAITWSFSWSVYRGPAEVTVNAGDSAATVAARLPTLRVEVWPARAVDGVVRFGSAP
                     LVAQGARTEPITTLHYTLPALRDPLTTMAFVVGIAPDGRRVILDEKLDLTRTPPWTGT
                     LAEWWTGG"
     gene            1647..2390
                     /locus_tag="VHM48_06200"
     CDS             1647..2390
                     /locus_tag="VHM48_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755034.1"
                     /translation="MDDLAVGRLLRLARSRRRLRQSDVARIAGVHQGTVSLAERGQLG
                     ALTVRTTRAIAAALEIGLQFEPRWRGPALDRLRDAGHAAAVEAVAAELARHGWDVLIE
                     VGFSRYGERGSVDVVGWRATESALLIVEVKTSLVDLQATLGAIDRKRRIVPGELSRER
                     GWRPTAIGVALVVVDSSTARDVVTRHRSIFDSAFPSRTIAVRQWLESPTGDLRGLWFL
                     RPTRKVRTTGRIDSSPTLPGASRVRPRRS"
     gene            2539..>2727
                     /gene="ffs"
                     /locus_tag="VHM48_06205"
     ncRNA           2539..>2727
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHM48_06205"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          339 a          972 c          998 g          418 t
ORIGIN      
        1 cgtcgtgcgc gagagcgagt tgtacggcta ccagatcgcc cagcggatcc gcgagcggag
       61 cgacggagca ttcgtgccga gcgaagggtc gctctacccg acccttcacc ggctcgaggc
      121 ggacggtgcc ctgacggcga cgtggcgcga gggcgagcgc ggccctcgcc ggcgctggta
      181 ccggatcacg ccctccggcg tcgcgacgct cgccgctcac gaggccgagt gggaggcgtt
      241 cagcgatgcg gtgacgcggg ttgcgtcggt tgcgcgggag ccgtccggtg cctgaccccg
      301 gcgagcgcga cgccttcctc gcgtcggtgc gctcccgcct ggacctgccc gacgcggtcg
      361 cgcgcgacat cctggaggag ctctccggcc acctcgagga cgcagccgcc gagctgcggg
      421 ccgccggcct ctccgccgac gatgccgaac gacgagcgat cggccgactg ggcgacgcgc
      481 gggcgctggg gacggagctg gcgcgggccc gtcgcggacg acgccagctg ctggcggccg
      541 tgggcggcgg gattcgggcg gtcctcgtgg agggaatccg gacctgggtc ttcctcgcgg
      601 tgctcgccag cctgacggcg atcctggcgc tgcccgcggc gtcgatcgtc cggctccttc
      661 tcggtcttcg atccacgtcg agcatgctcg tcggcatgcc cggcagcctc gcaaccgtcg
      721 gcgcggtcgt cggcgggttc gcctatctcg gatgggtcct gcctgcccgg ttcgccgggc
      781 cggcagtacg gtcggtgcgc ggtgtccggc gggccgtcgc gttggtgggg ctcgcggtcg
      841 ggtcggctat cctctggttc ctggtgcccg tcgcgatgga cccggtgctg gtcgtcggcc
      901 tgccgctcgg cccggtggca ttcgctctta ccgcgctccg ggcgccggag cggccaacca
      961 tccgcgtggg cttcgtcccg gcggtgattg gcgccggcat cctgttcctg ccgatgacgc
     1021 tgcttgcgct cgcggcgacc gacccgcctt tggacaactg gatggccgac atctccccga
     1081 tcggggacgt gcccgccgcc acggacatcg agaacaccgc cctggcgata acctggtctt
     1141 tctcatggtc cgtctaccgc ggcccggccg aggtgacggt gaatgccggc gattccgcgg
     1201 caacggtagc cgcccgcctc ccgacgctcc gggtcgaggt ctggccggcg cgcgccgtgg
     1261 acggcgtcgt ccgattcggg tccgccccgc tggtcgccca gggcgcgcgg acggagccga
     1321 tcacaactct gcactacacg ctgccggcgc tgcgcgaccc cctgacgacg atggccttcg
     1381 tcgtggggat cgctcccgac gggcgccggg tcatcctcga cgagaaactg gatctcaccc
     1441 ggacgccgcc ctggaccggc acgctcgcgg agtggtggac ggggggctga ccctcggtcc
     1501 gggcgcgacg gcggagccgg ggacgcgccg tctcggtccg cggccgcgtc gtctcggccc
     1561 gcggccgcgt cgcggccgtg cgtcagtgca tcggcggtgc aggagccggt aacggcgagg
     1621 caatgccggc gcgccatgat cgacccgtgg acgatctcgc agtcggtcgg ctgctccgtc
     1681 tcgctcgcag tcgacgccga cttcgacagt ctgatgtcgc ccggatcgcg ggcgtccacc
     1741 agggcaccgt ctcgctcgcc gaacgcggac agctcggtgc gctgacggtc cggacgacgc
     1801 gtgcgatcgc cgcggccctc gagatcggac tgcagttcga gccgcgctgg cgcggtcccg
     1861 cactggaccg gttgcgcgac gccggtcacg ccgcggcagt ggaggcagtc gcggccgagc
     1921 tcgcccggca cggctgggac gtgctcatcg aggtcggctt cagccgctac ggcgagcgcg
     1981 gcagcgtcga cgtggtcgga tggagggcaa ctgaaagtgc gctcctgatc gtcgaggtca
     2041 agacgtcgct cgtcgacctc caggcgacgc tcggcgcgat cgatcggaag cgccggatcg
     2101 tgccgggaga actgtcacgt gaacgaggat ggcggccgac cgccatcggc gtcgctctcg
     2161 tggttgtgga ctcgtcgacc gcgcgggacg tggttacccg ccaccgatcg atcttcgact
     2221 ccgcgttccc gagcaggacc atcgcggtgc gtcagtggct ggagagcccg accggagacc
     2281 ttcgaggcct gtggtttctc cgacctacgc gcaaggttcg tactacggga cggatcgact
     2341 cgtcgccaac gcttccgggc gcttcacggg tccggccgcg ccgctcctga gcacgacgcg
     2401 ggcagcccag cccccgggac aggaccccag ctgctcgggg tcctccgtag tacgaactgg
     2461 gcgcgtattt cggaagaacc ggccggcgtc cccgcagggc cgaaggccct cccgggtccc
     2521 cctgctatga tcccgctgcc gtgctaggcg gggaactagc ggtgccctgt acctgcaatc
     2581 cgctccagca gggctgaatt cccccccgag gtcttcgtct tcgagagccg ccggcgcgaa
     2641 cgacgccgag gatcgggtcc cgcgcagcgg cgacacgcga accgcgtcag gtccggaagg
     2701 aagcagcgct aagcgaggct cgtcggg
//
LOCUS       DATAIW010000193        11614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_159183, whole genome shotgun sequence.
ACCESSION   DATAIW010000193 DATAIW010000000
VERSION     DATAIW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159183"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHM48_06210"
     CDS             complement(<1..226)
                     /locus_tag="VHM48_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755035.1"
                     /translation="MSVDAMENVPPEDWPVVLANLGRALRTADHLYLTVEEVSDADID
                     AAFSANRSHGWPAVRGEIIEGDTAGYHYYPG"
     gene            complement(596..1690)
                     /locus_tag="VHM48_06215"
     CDS             complement(596..1690)
                     /locus_tag="VHM48_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755036.1"
                     /translation="MIAIQVLAFLLGAGAAIRVVFSAVRTFVVPRGDNDGLSRFFFRA
                     MRKGFDLLARPSRPYEFRDRVMAYYGPISLVALPAFWLVVLAAAFATMFWAIGLPLGE
                     AFVVSGSSLLTLGFARPAVPGGDLLAFAEAAIGLALVALLVSYLPTIYGAFSRRELLV
                     NLLEVRADSPPSPIVMITRMDRLSGLAVLHDLWISWEKWFAELEETHTSLPVLVFYRS
                     QQNNHSWVNAAGAMMDAAALIRSAVAVESDVQADLMIRAGYLALRRIAAYFRIAFDPN
                     PAPTSATSIDRARFEEALTVLEDAGVPLVEDRDQAWLDFNGWRVNYDTVLRALERLTM
                     APTPWWERPMRSAYATDEPALIEAAPTVVA"
     gene            1965..2111
                     /locus_tag="VHM48_06220"
     CDS             1965..2111
                     /locus_tag="VHM48_06220"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX2755037.1"
                     /translation="MLWTILVILVVLWALGLVGSIGGGLIHLLLVLAVIVLAVQLLQG
                     RRSA"
     gene            complement(2218..2982)
                     /locus_tag="VHM48_06225"
     CDS             complement(2218..2982)
                     /locus_tag="VHM48_06225"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2755038.1"
                     /translation="MNDSTFSIAGQVALITGATGGIGADLAAAFGRAGARVGVAGRRM
                     DAAEAVVERIRGDGGEAAAVELDLTDRASIDEAVEATVQRFGRIDILVNNAGLGTNHD
                     AIDATEAEWDDLFAVNVRGLFFACQSAARRMLPQGSGRIINMASQAGLVGIERHAAYS
                     ASKAAVIGLTRVLALEWSPHGVTVNAVAPTWTYTPGTAERLDEPAFLASVLARIPAGR
                     VATTDEIAGAFLYLASPAAAMVTGSVLTIDGGWTAR"
     gene            3054..4085
                     /locus_tag="VHM48_06230"
     CDS             3054..4085
                     /locus_tag="VHM48_06230"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2755039.1"
                     /translation="MTERILSPRALNRALLARQLLLEGSPLSITDAIERIGGLQTQYA
                     PSGYVGLWTRLAAFRREALTEALDDRSVVQATLLRTTIHMVSRREFWLYAMGIREARR
                     AWALRLGPNAAVEASLPAAADRIRAALAAGPRTVRELDGLVTGFVGKASLWVDLVRVP
                     PSGTWERRRADRLGLAEDWVGPPDATEDEGLAHLVRAYLRAFGPAPWRDIALWAGMSG
                     ADVRRGGEGVRLVRYRDEAGKELVDLPNAPLPDPDTPAPVRFLPHWDAVLLVHARRTG
                     ILPEEHRGKVFQTRNPFSVGTVLVDGRVVAGWSLRDGRIVVDPYEEIAAADRRAVEDE
                     GAALEAFHA"
     gene            4283..4603
                     /locus_tag="VHM48_06235"
     CDS             4283..4603
                     /locus_tag="VHM48_06235"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX2755040.1"
                     /translation="MAKYLFRASYTAAGAAGVLKDGGSSRAKAVEALVASVGGTIESL
                     YWSFGADDFFMIADVPDSHAAAALSLTVGASGGASVTTSELLSAADVDDIAGRRVTYR
                     PPGA"
     gene            complement(4659..4862)
                     /locus_tag="VHM48_06240"
     CDS             complement(4659..4862)
                     /locus_tag="VHM48_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755041.1"
                     /translation="MVEYGNGVGQATGAAGGTGGGQTMDAGAAFGQFVGNAVHTISTM
                     PPTQLALLVVIVVVGLVIFKRAF"
     gene            complement(4939..5127)
                     /locus_tag="VHM48_06245"
     CDS             complement(4939..5127)
                     /locus_tag="VHM48_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755042.1"
                     /translation="MIPRGTRHQFRTLVAPSRILLLIVPAGLEGFFREMGSEIAAGRS
                     PLEALTALSARYGSHPLD"
     gene            5258..6436
                     /locus_tag="VHM48_06250"
     CDS             5258..6436
                     /locus_tag="VHM48_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2755043.1"
                     /translation="MRHLRRLLARLPWVPDRGVDGQAAIEEPPPPSEPAIAATPGALP
                     PPVRDRAPFAVLLLAAIGILWISRGVLGPFIVAAVLAYAFSPLVSAGQRRLRWPRIAV
                     VGLGYAIAAAIVAVLVLVLADRVAREFQLLAGSGPDSLARTLRELVGSDKLAIGGQQI
                     AVADIAREIQTRIGGLIASPGDALHIAGQVGEVGLQMILAVIVTFYFLVDGTMFMDRA
                     IAILPAGHRARTTEVLARIHVILGKWLRGQLLLIALVAAVAYVGLGPVLHLPYALALA
                     VLTGILEIIPLIGPLVATAIVAVDAFARDGAGLAAAVIVFYFVLRQVEDQVVMPVVIG
                     RAVHLHPVVTIFAVLVGLSLYGILGGLLGVPVAAAINVVFRELYASPAEDAEPESTGT
                     "
     gene            6433..7026
                     /locus_tag="VHM48_06255"
     CDS             6433..7026
                     /locus_tag="VHM48_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755044.1"
                     /translation="MSPDGKASPEPSIDAGRVRLRRLATAELSVDELQAIRALLDAAF
                     GDDEDGRFTDDDWQHSLGGMHFVLDVDGRLVAHASVVEREIHVDGWPLRTGYVEAVAT
                     APGFQGAGFGSIVIADVTAFVRERYEFGVLGTGRHHFYERLGWLTWEGPLLVRTADGS
                     RRTPEDEGFLLVLRTPTTPPIDIHAPISCDWRPGDVW"
     gene            7076..7453
                     /locus_tag="VHM48_06260"
     CDS             7076..7453
                     /locus_tag="VHM48_06260"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX2755045.1"
                     /translation="MSEAPDRPADIEAWFGRLPEDQQSALRGLWATIAAAAPTAVETI
                     GYGVPALHYRGRALVSIGAAKAHCSLFVQSTAVMEAHAAELAGYPTSKGAVRFTPETP
                     LPDDLVATLVRARIAEVDRALER"
     gene            complement(7495..7872)
                     /locus_tag="VHM48_06265"
     CDS             complement(7495..7872)
                     /locus_tag="VHM48_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755046.1"
                     /translation="MSSWLVPVVGFVIGIAMAAALLGRDASPIQAVIAFAIMAGYALG
                     LRFLQSRSDVASVLSGLPPDERWASINLQALSLTAQLLAVVLVLAFLVTVFARGDATT
                     FAWLGAVLGATYLGGLVWYRSHS"
     gene            complement(7865..8065)
                     /locus_tag="VHM48_06270"
     CDS             complement(7865..8065)
                     /locus_tag="VHM48_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755047.1"
                     /translation="MTNEIRSLREGRGLSQGQLAAELGVSRQTINAIETGRYLPSLPL
                     AFAIARYFARRIEEVFHDRADV"
     gene            complement(8185..8811)
                     /locus_tag="VHM48_06275"
     CDS             complement(8185..8811)
                     /locus_tag="VHM48_06275"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEX2755048.1"
                     /translation="MTASRRSLVVVSAGLGQPSSTRLLADRLIAAVERHLGDAGLEPR
                     VEIIELREHAKDLTNHLLTGFPSPKLQATIDAVLGADGLIAVSPIFSATYSGLFKLFF
                     DVVARDGLAGMPTLIAATGGTVRHSLALEHGFRPLFAYLNAATVATSVFAAAEDWGTA
                     GVAADGSLVERIDRAARELAAAISRREPRPATDRFADPLPFEDLLRSQ"
     gene            complement(8808..9932)
                     /locus_tag="VHM48_06280"
     CDS             complement(8808..9932)
                     /locus_tag="VHM48_06280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2755049.1"
                     /translation="MQFGVFSVGDITPDPTTGRAVGETERIQIMMTVALKAEEVGLDV
                     FATGEHHNPPFISSSPTTMLGFIAARTQRLLLSTATTLITTNDPVKIAEDYAMLQHLA
                     DGRVDLMMGRGNTGPVYPWFGQDIRNGIPLAYEHYALLHRLWREDVVDWEGKLRTPLT
                     GFTSVPRPLDGVPPFVWHGSIRSPEIAEQAAYYGDGFFHNNIFWPPDHVKRMVELYRR
                     RWEHYGHGPADQAIVGLGGHVFMRKNSQDAIREFRPYFDHAPVYGGGPSLEDYMAQTP
                     MTIGSPQEVIDRTMSFRDIVGDYQRQLFLADHAGLPLKTVLEQMDMLGELVVPVLRRE
                     FAALKPAHVPEAPVHPRVAAARVADDAPVDAPSPATAVPA"
     gene            10151..11056
                     /locus_tag="VHM48_06285"
     CDS             10151..11056
                     /locus_tag="VHM48_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755050.1"
                     /translation="MIVRRWRSPSDDDPRRSIAIASPRRFWYTKRGRSNPLRRIAGAS
                     DPLEAVSEDARRALVLHDRALVIDLIELTLNHGPFIVRSAGGLAGAEAILTEWRPHLC
                     VVDMDHDDSARLLRHIGASNTLTRSLIPVLGLTRRGDLKTKLRAFDLGVDDIVTLPFS
                     PEELLARAIVVSRRASGVDRAIVPTITVGEMVIDIVNREVRAGQSVVHLSGIEQSLLY
                     LLASRAGKVVTRDEILDVIWGTDFVAESNIVDRHVRSLRIKLQDDYRHPRFIATVPGR
                     GYRFIPTFSNHGWDVTDGSAPPTTA"
BASE COUNT         1709 a         4075 c         4092 g         1738 t
ORIGIN      
        1 gtcccgggta gtagtggtag ccggcggtgt cgccttcgat gatctcgcca cggaccgcag
       61 gccacccgtg cgaccggttc gcgctgaagg cggcatcaat gtcggcgtcg ctcacctcct
      121 cgacggtcag gtacagatga tcggcggtcc ggagggcccg gccgagattg gcgaggacga
      181 ccggccagtc ttccggcggg acgttctcca tggcgtcgac ggacatcgcc gcgtcgaact
      241 cgtggtcgaa aggccatctc ctgagagccc gacgtgctcc agtcggccgg cgatgccgtt
      301 cttgcgcgcc tgagcgagcg tggcgctgga ctggtcgatg ccaaccacgc gccggccgga
      361 ggcgtgcacc atctcgaaat agcgtcccgt cccgcacggg gcgtcgagga cgatcccgcc
      421 ggccgggcag gtcgcgagca accgctccac gaatgatcgg tgcgaggtgg cgggatagag
      481 gtgctcgtcg tatgccgctg cttccgcgtc gtactcggcg gtctccgctg cccggcgcgc
      541 agcaagccac gcagcccggg cccggtcgtc gttcgcggcc gccatctccg gaccttcagg
      601 cgaccacggt cggcgcggcc tcgatcagcg ccggctcgtc ggtcgcgtag gccgagcgca
      661 tcgggcgctc ccaccacggc gtcggcgcca tcgtcaggcg ctcgagcgcg cgcaggacgg
      721 tgtcgtagtt gacccgccag ccattgaagt cgagccaggc ctggtcgcga tcctcgacca
      781 acggcacgcc ggcatcctcg agaaccgtca gggcttcctc gaaccgcgca cggtcgatac
      841 tggtcgcgct cgtcggggca gggttcgggt cgaaggcgat ccggaagtac gccgcgatcc
      901 ggcgcagggc gaggtagccc gcccggatca tgagatcggc ctggacgtcg ctctcgaccg
      961 cgaccgccga ccggatgagg gcagcggcgt ccatcatcgc ccccgccgcg ttgacccacg
     1021 agtggttgtt ctgctgcgac cgatagaaga ccaggaccgg cagcgaggtg tgcgtctctt
     1081 cgagctcggc gaaccacttc tcccagctga tccacaggtc gtgcaggacg gcaaggccgg
     1141 acaggcggtc catccgggtg atcatcacga tcggcgacgg aggcgaatcc gcccggacct
     1201 cgagcaggtt gacgagcagc tcgcgacgcg agaacgcgcc atagatcgtc ggcaggtagg
     1261 aaacgagcag agcgacgagg gcgagcccga tcgccgcctc cgcgaacgcg agcagatcgc
     1321 cgcccgggac cgccggccgg gcgaatccga gcgtcaacag ggacgaaccg ctcacgacga
     1381 acgcctcgcc gagcggcagg ccgatcgccc agaacatcgt cgcgaatgcc gctgcgagca
     1441 cgacgagcca gaaggccggg agtgccacga gcgagatggg tccgtagtag gccatgacgc
     1501 ggtcgcgaaa ctcgtacggt cgcgacggcc tggcgaggag gtcgaagcct ttccgcatgg
     1561 cgcggaagaa gaatcgtgac agcccgtcgt tgtcgccacg gggaacgacg aacgtgcgga
     1621 cggcgctgaa gacgacccgg atcgccgcgc cagcgccgag gaggaaggca aggacctgga
     1681 ttgcgatcat ccgcgcagga tacctaccgc cacgaccgcc gggcgctcgc cggtcgagcg
     1741 ttcgggctac ccgagtcatt cctcgacgct ccgccgccgg gcgaagaccg cccggctgtc
     1801 gccggatcaa gtcgtgccgg ctggcgggcg gcgcaactgc aatgacgatg ccagcgtttg
     1861 gacggtcgcc ggtctgaccg tcaacgtgct ggtgccgaag atgggaagcg acgcgatccg
     1921 catccgctcc gggagacggc gcgcacgctc gattggagag ctccatgctc tggaccatcc
     1981 tcgtgatcct tgtcgtcctc tgggcgctcg gcctcgtcgg aagcatcggc ggcgggctca
     2041 tccacctgct cctggtgctg gccgtgatcg tccttgccgt ccagctcctc cagggccgcc
     2101 gctcggcctg aacgcggcga cggcccgtgg tttcggccgg gccgtcgtca acggacggcg
     2161 tgcctcctgc gcgaggttcg cccggttcgc aacgtcgagc cgacgagaat ctggcgccta
     2221 gcgcgccgtc cagccgccgt cgatcgtcag gaccgagccg gtgaccatcg ccgcggctgg
     2281 cgacgcgagg tacaggaagg ccccggcgat ctcgtcggtc gtggcgaccc gaccggccgg
     2341 gatgcgggcc aggaccgagg cgaggaacgc cggctcgtcg agccgctcgg ccgtgcccgg
     2401 ggtgtacgtc cacgtcggcg cgacggcgtt gaccgtgaca ccatggggcg accactcgag
     2461 ggcgaggacg cgggtcaggc cgatcaccgc cgctttgctc gcggagtagg cggcgtggcg
     2521 ctcgataccg acgaggcccg cctgcgacgc catgttgatg atccgcccgg aaccctgggg
     2581 cagcatccgc cgggccgccg actggcaagc gaagaacagg ccgcggacgt tcaccgcgaa
     2641 caggtcgtcc cattcggcct ccgtcgcgtc gatcgcgtcg tggttcgtgc cgagcccggc
     2701 gttgttgacc aggatgtcga tccgcccgaa ccgttggacg gtcgcttcga ccgcctcgtc
     2761 gatcgaggcg cgatcggtca ggtcgagctc gactgcggcc gcctcgccgc cgtcaccacg
     2821 gatccgctcg acgaccgctt cggccgcgtc catccgccgg ccggccacgc cgacccgcgc
     2881 tccggcccgc ccgaaggcgg ccgccaggtc ggccccgatc ccgccggtgg cgccggtgat
     2941 cagcgcgacc tggccggcga tcgagaaggt cgaatcgttc atcgcatgag ggtacccagc
     3001 ggccggctcg tccgtgacgc gacggcgact cgccaccccg gcagaatggc atcgtgaccg
     3061 agcgcatcct ttcgccgcgc gccctgaacc gggcgctcct cgctcgccag ctcctcctcg
     3121 aaggctcgcc gctgtcgatc acggacgcga tcgaacggat cggcggactc cagacgcagt
     3181 acgcgccgtc aggctacgtc ggtctctgga cccggctcgc cgccttccgt cgagaggccc
     3241 tcacggaagc gctcgacgat cggtcggtgg tccaggcgac gctcctgcga acgacgattc
     3301 acatggtgtc gcggcgcgag ttctggctct acgcgatggg gatccgcgaa gcgcgccggg
     3361 catgggcact tcgcctcggg ccgaatgcgg cggtcgaggc atccctgcct gctgccgccg
     3421 accgcatccg ggccgcactc gccgccggcc cgcgcaccgt cagggagctc gatggactcg
     3481 tcaccgggtt cgtcggcaag gccagcctct gggtcgacct cgtccgggtc ccgccgtccg
     3541 ggacgtggga gcgtcggcga gccgatcggc tcggtctcgc cgaggactgg gtcgggcccc
     3601 ccgacgcgac cgaggatgag ggcctcgccc acctcgtgcg cgcatatctc cgcgcgttcg
     3661 ggcccgcgcc gtggcgcgac atcgcgctct gggccgggat gtccggcgcg gacgtgaggc
     3721 ggggagggga gggcgtgagg ctggtccgct atcgcgacga ggccggcaag gagctcgtcg
     3781 acctgcccaa cgcgccgctg ccggaccccg acacgccggc gcccgtccgc ttcctgccgc
     3841 attgggatgc ggtcctgctc gttcacgccc ggcgaaccgg catcctgccc gaggagcacc
     3901 gcggcaaggt gttccagacg aggaatccgt tctccgtcgg gacggtcctc gtcgacggcc
     3961 gcgtcgtggc cggttggtcg ctgcgtgacg gccggatcgt ggtcgacccg tacgaggaga
     4021 tcgccgcggc cgaccgtaga gcggtcgagg acgagggcgc cgcactcgag gccttccacg
     4081 cctgacgggc gcgcccgttc gcggggcgat cgcccgggct cgaccgggct ccacatccgg
     4141 agcgggatgc ctgctggttc ccgcggcctg gcacggtctg gtacgacctg tgtggcgaga
     4201 ctccgtcgtt cgtgcgactt cgtgggcgta ggatcgaccg acgccgctga cgtccagcgg
     4261 cgaccacgaa accgaggacg agatggcaaa gtatctgttc cgggcgtcgt acacggcggc
     4321 cggtgcggct ggagtcctca aggacggtgg gtcgtcgcgg gcgaaggccg tcgaggcgct
     4381 cgtcgcgagc gtcggcggca ccatcgagtc gctgtactgg tcgttcggtg ccgacgactt
     4441 cttcatgatc gcggacgtgc ccgattcgca cgcggcggcg gccctgtcgc tgacggtcgg
     4501 cgccagcggc ggcgcgagcg tgacgacgtc ggagctgctg tcggcggcgg acgtcgacga
     4561 catcgccggc cgacgggtga cctaccggcc gcctggcgcc tgaccgggac gttcgtcgag
     4621 ccggccggcg cgatccgacc ggctcagacg gcgggccttc agaatgcgcg cttgaagatg
     4681 acgaggccga cgacgacgat cacgacgagg agcgcgagtt gggtcggcgg catcgtcgag
     4741 atcgtgtgga ccgcatttcc gacgaactgc ccgaaggcgg cgccggcgtc catcgtctga
     4801 ccgccaccgg tgccgccggc ggcaccggtt gcctggccca cgccgttccc gtactcgacc
     4861 atgcccggca tcatgcgctg acccgttcgc cgtgacaaga ccgtggagtc gacggtggcg
     4921 ggtgaagtgc ggtcccggtc agtccagcgg gtgcgagccg tagcgcgccg agagcgcagt
     4981 cagcgcctcg agcggcgacc gcccggccgc gatctcgctg cccatctcgc ggaagaaccc
     5041 ttcgagaccc gccggcacga tcaggagcag gatcctcgat ggcgcgacga gggtccggaa
     5101 ctggtgacgc gtgccgcgcg ggatcacgat ggaagcgccc gcttcgagct cgacctcctc
     5161 atgtcccgaa tgaacacgta cctgccctcg aggacatgga acaactcctc gtggtggcgg
     5221 taaacgtgcg ggggcggcag gactgtactc tcgcgccatg cggcacctcc ggcgactcct
     5281 ggccaggctc ccgtgggtgc ccgatcgagg ggtcgacggg caggccgcga tcgaggagcc
     5341 gccgccgccg tccgagccgg cgatcgccgc gaccccgggg gcactcccac cgccggtccg
     5401 cgatcgggcg ccgttcgcgg tcctcctgct cgctgcgatc gggatcctgt ggatttcgcg
     5461 gggcgtcctc gggccgttca tcgtcgccgc cgtcctggcc tacgccttca gcccactggt
     5521 cagcgcgggt cagcggcggc tccgctggcc gcggatcgcg gtggtcgggc ttggctacgc
     5581 aatcgcggcg gcgatcgtcg ccgtcctcgt cctcgtgctg gcggaccggg tcgcccggga
     5641 gttccagctc ctcgccggct ccgggccgga ctccctggcg aggacgctgc gtgagctcgt
     5701 cggcagcgac aagctcgcga tcggcggcca gcagatcgcc gtcgccgaca tcgctcgcga
     5761 gatccagacg cggatcggtg gcctgatcgc atccccgggc gacgcgctcc acatcgccgg
     5821 ccaggtcggc gaggtcggtc tgcagatgat cctcgcggtc atcgtcacgt tctacttcct
     5881 cgtcgacggg acgatgttca tggaccgcgc gatcgccatc ctgccggccg gtcaccgggc
     5941 ccggacgacc gaggtcctcg ctcggatcca cgtcatcctc ggcaagtggc tgcgcggcca
     6001 gctcctgctg atcgccctcg tcgcggccgt ggcctacgtc ggacttgggc ccgtcctcca
     6061 cctgccctac gcgctggccc tcgcggtgct gaccgggatc ctcgagatca tcccgctcat
     6121 cggaccgctg gtggcgacgg cgatcgtggc cgtcgacgcg ttcgcccgcg acggcgccgg
     6181 cctcgcggcc gcggtcatcg tcttctactt cgtcctccgc caggtcgagg accaggtcgt
     6241 catgccggtc gtgatcgggc gggccgtcca cctccacccg gtcgtcacga tctttgccgt
     6301 cctcgtcgga ttgagcctgt acggcatcct cggcggcctg ctcggcgtgc cggtcgccgc
     6361 cgccatcaac gtcgtcttcc gcgagctgta cgcgagcccg gcggaggatg cggagcccga
     6421 gtcgaccgga acgtgagccc ggacgggaag gcatcgcccg agccatcgat cgacgccggg
     6481 cgggttcgcc ttcgtcgtct cgcgaccgcc gagctcagcg ttgacgagct ccaggcgatc
     6541 cgggcgctgc tggacgccgc attcggggac gacgaggacg ggcgattcac cgacgacgat
     6601 tggcagcatt ccctcggcgg gatgcatttc gttctcgacg tcgatgggcg gctcgtggct
     6661 catgcctccg tcgtcgagcg cgagatccac gtcgacggat ggccactccg caccggctac
     6721 gtcgaggcgg tcgcaaccgc gcctggattc cagggagccg ggttcggatc gatcgtcatc
     6781 gccgacgtga ccgccttcgt ccgcgagcgc tacgagttcg gcgtgctcgg gaccggtcgc
     6841 caccacttct acgagcggct gggctggctc acctgggagg gaccgctcct cgtcaggacg
     6901 gccgacggct ctcggcgcac gccggaggac gagggcttcc tgctggtcct gcggacgccg
     6961 acgacgccgc cgatcgacat ccacgccccg atcagctgcg actggcgccc cggcgacgtc
     7021 tggtaggcgg tcgatgccgg cgtgccgcta cgatcgacgg catggagggc acccgatgag
     7081 cgaagctccc gatcgacccg cggacatcga ggcctggttc gggcgattgc cggaggacca
     7141 acagtccgcc ctgcgcgggc tgtgggcgac gatcgctgcc gccgcgccaa ccgcggtcga
     7201 gacgatcggt tacggcgtcc cggcgctcca ctaccgcggc cgggccctgg tctcgatcgg
     7261 tgcggcgaag gcccactgct cgctcttcgt ccagagcacc gccgtcatgg aagcccacgc
     7321 ggcagagctg gcgggatacc cgacctccaa gggcgccgtc cgcttcacgc cggagacgcc
     7381 gctcccggac gacctcgtcg ccactctcgt ccgggcgcgc atcgcggagg tcgatcgagc
     7441 gctcgaacgg tgagagcccg gccgctcacg tgagggcact cccgttgcgg ccgctcagga
     7501 gtggctgcga taccagacga ggccgccgag gtaggtcgcg ccgaggaccg caccaagcca
     7561 cgcgaacgtc gtcgcgtcgc cgcgagcgaa cacggtcacg aggaacgcca gaaccaggac
     7621 gacggcgagc agctgggccg tcagcgacag ggcctgcagg ttgatcgacg cccagcgctc
     7681 gtcaggcggc agccccgaga ggacgctggc gacgtccgag cgggactgga ggaaccgcag
     7741 gccgagggcg tagccggcca tgatcgcgaa cgcgatcacc gcctggatcg ggctggcatc
     7801 gcggccgagc agggccgccg ccatcgcgat gccgatgacg aacccgacca ccggcacgag
     7861 ccagctagac atccgcgcgg tcatggaaga cctcctcgat ccgacgtgcg aaatagcggg
     7921 cgatcgcgaa cgcgagcggc agcgagggca ggtaccgacc ggtctcgatc gcgttgatcg
     7981 tctggcgcga gaccccgagc tccgcggcaa gctgcccctg gctcaggccg cgaccttcgc
     8041 ggaggctccg gatctcgttc gtcatgctgt caagttacct tgacagcata gcgcggtgac
     8101 aaggtccttt gtcaccttcg atcgggcgga atggctcgga tggccgaggt cggcgaggcg
     8161 ccgggtcgtt gacgtcgcgg atccctactg cgacctgagc aggtcctcga agggcagcgg
     8221 atccgcgaac cggtccgtcg ccgggcgcgg ctcgcgccgc gaaatcgccg ccgcgagctc
     8281 ccgggccgcg cgatcgatcc gctcgaccag gctcccatcg gcggccacgc cggcggttcc
     8341 ccagtcctcg gccgcggcga agaccgacgt cgcgaccgtc gctgcgttga ggtaggcgaa
     8401 gagcggccgg aagccgtgct cgagggcgag cgaatggcgg accgtgcccc cggtcgccgc
     8461 gatcagggtc ggcatcccgg ccagcccgtc ccgtgcgacg acatcgaaga agagcttgaa
     8521 caggccgctg tacgtggcgc tgaagatcgg gctgaccgcg atcaggccgt cggcgccgag
     8581 gacggcgtcg atcgtcgcct ggagcttcgg gctcggaaag cccgtcaaca ggtggttcgt
     8641 caggtccttg gcgtgctcgc gcagctcgat gatctcgacg cgcggctcca ggccggcgtc
     8701 gccgaggtgc cgttcaaccg ccgcgatcag acggtcggcg aggaggcgcg tcgacgacgg
     8761 ctgaccgagc cctgcgctga cgacgacgag cgaacgcctc gaggccgtca tgccggcacg
     8821 gccgtcgccg gtgacggcgc atcgaccggt gcgtcgtctg ccacccgcgc ggcagcgacg
     8881 cggggatgaa cgggcgcctc cgggacgtgc gcgggcttga gggcagcgaa ctcccgacgg
     8941 aggaccggca cgacgagctc gccgagcatg tccatctgct cgaggaccgt cttgagcggc
     9001 agcccggcgt ggtcggcgag gaacagctgg cgctggtagt cgcccacgat gtcccggaat
     9061 gacatcgtcc gatcgatcac ctcctgggga ctcccgatcg tcatcggcgt ctgggccatg
     9121 tagtcctcca gcgacggtcc gccgccgtac accggagcgt gatcgaagta cggccggaac
     9181 tcgcggatcg catcctgcga gttcttgcgc atgaacacgt gcccgcccag acccacgatc
     9241 gcctgatccg ccggcccgtg gccgtaatgc tcccagcgcc gacggtacag ctcgaccatg
     9301 cgtttcacgt ggtcgggcgg ccagaagatg ttgttgtgga agaagccatc gccgtagtag
     9361 gccgcctgct cggcgatctc cgggctgcgg atcgagccgt gccagacgaa cggcggcacg
     9421 ccgtccaggg ggcgggggac cgaagtgaag cccgtcagcg gcgtccggag cttgccctcc
     9481 cagtcgacga cgtcctcgcg ccagagccgg tggaggaggg cgtagtgttc gtacgccagt
     9541 gggatcccgt tgcggatgtc ctggccgaac cacgggtaga cggggccggt gttgccgcga
     9601 cccatcatca ggtcgacccg gccgtcggcc aggtgctgga gcatcgcgta gtcctcggcg
     9661 atcttgaccg gatcgttggt cgtgatcagg gtcgtcgcgg tcgacagaag gagtcgctgc
     9721 gtccgggccg cgatgaagcc gagcatcgtc gtcggcgagg aggagatgaa cggcgggttg
     9781 tggtgctcgc cggtggcgaa gacgtcgagc ccgacctcct cggccttgag ggcgaccgtc
     9841 atcatgatct ggatccgctc ggtctcgccg accgcgcgtc ccgtcgtggg gtcgggcgtg
     9901 atatcgccga cggaaaagac cccgaattgc atctcgactc ctggtgctcg acgcgacccg
     9961 tgccgcgccc cggctcgatg ttccatcgtg cctctcgcag cctacgatat cccaatgtcg
    10021 ttgcacatgc aacgacctgc cccgaggtgc gtcacggacc caggttggac ccggcgatgc
    10081 tcggttcgga ggcgcgccac ggcgggctcg gccgtcggca acccgggtgc tgaaggtagc
    10141 ggcctcggcc gtgatcgtcc ggcgatggcg gtcgccttcg gacgacgatc cgcgtcgatc
    10201 gatcgcgatc gcgagccctc gccgattctg gtataccaag cgtgggcggt cgaacccgct
    10261 ccgtcgcatc gcaggagcat cagacccgtt ggaagccgta tcggaagacg cccgtcgagc
    10321 gctcgtcctg cacgaccgtg cgctggtgat cgatctgatc gagctgacgc tcaaccacgg
    10381 cccgttcatc gtgcggtcgg ccggcggtct tgccggcgcc gaggcgatcc tcaccgagtg
    10441 gcgtccgcac ctgtgcgtcg tcgacatgga tcatgacgac agcgcccgcc tgcttcgcca
    10501 catcggcgct tcgaacacgt tgaccaggag cctcattccg gtgctcggcc tgacccgccg
    10561 cggcgacctg aaaaccaagc tccgcgcctt cgatctcggc gtcgacgaca tcgtgacctt
    10621 gccgttctcg ccggaggagc ttcttgctcg ggccatcgtc gtgagccgcc gcgcttcggg
    10681 cgtcgaccgc gcgatcgtgc cgacgatcac cgtcggcgag atggtgatcg acatcgtgaa
    10741 ccgcgaggtc cgggctggtc agtcggtcgt ccacctgagc ggaatcgagc agagcctcct
    10801 gtacctgctc gccagccgag ccggcaaggt cgtgacccgc gacgagatcc tcgacgtgat
    10861 ctggggcacg gacttcgtcg ccgagagcaa catcgtcgac cgccatgtcc ggagcctccg
    10921 gatcaagctc caggacgatt accgtcatcc gcgcttcatc gcgacggtgc cgggtcgcgg
    10981 gtaccggttc atcccaacgt tctcgaacca tggctgggac gtgaccgatg ggtcggctcc
    11041 gcccaccacc gcgtaggcgc gcggcctgcg gcgccggatc cgtcgctcga tgaccgcgtt
    11101 gcagcggacg gccatgacgg cccgtaatga cgcccgggca cgaaggctgg atcgtcgcgc
    11161 ctgacccgca gcgcgcaccc cctgggcccg gtcaacaact tcgcaggacg ggtccgacac
    11221 gaggacgggc ccgtcctgca cctacagcag gctcggcggg agccgattgc gacggggtgg
    11281 acgacgggtc gtcggccgtg gtggcggcgg cccgtctccc attgcccggt agcagttgcc
    11341 cggcccgcgc ccggcccatg ggcccgcgac gcgaagctcg ggcgagcgcg gacctgggtc
    11401 ggcggcgtcg acggacccgg tcgatcgggg atttcctacc gtccccttcg ggccgaatgg
    11461 cacccgtccg cgaacctccg ctccggcgat ggtctgaggg ccaaccgcac cgaggagcgc
    11521 gcatgtccag tcaaccggtc cgtcccctcc gccgccagcg ccgtgccgcc gcgctcgccg
    11581 acaagcgggt tgatccgcgg cgcacggcgg gcga
//
LOCUS       DATAIW010000194         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_159348, whole genome shotgun sequence.
ACCESSION   DATAIW010000194 DATAIW010000000
VERSION     DATAIW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159348"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..886
                     /locus_tag="VHM48_06290"
     CDS             278..886
                     /locus_tag="VHM48_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755051.1"
                     /translation="MILRIVSGRVPAGRLDAVVEAYRRDYVPIARQTVGLDRFLVAAR
                     LVPDGGHEFAAMTIWTSVEAALAAYDGNLAAARTLDGQGHGETLTRVDYYEIDAGGAR
                     RRTGAATRLRLTAGTVARGLDADIQQELRRHLPDLPVEAIEAYVGRRVLGGAVEIALV
                     TTWSAAPAGVSLDAPLWPSIADRYDTFRIVVHDVLLDGAGAG"
     gene            999..1628
                     /locus_tag="VHM48_06295"
     CDS             999..1628
                     /locus_tag="VHM48_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX2755052.1"
                     /translation="MATTTRRSTSNRNGSSNGNGSARQKVSAQPRMGQTAPELQRFGT
                     LRQLPIALSAEARAESCQLLNEILADTMVLQALYKKSHWNVAGPTFYQLHLLFDKHSG
                     EQLELIDELAERVQMLGGISVGDPRHAAELTSIPRPPDGAEEVPVILSRLLDAHETII
                     EKVRDGLDKTEESKDLGTNDLLMGDVLRRHEMQVWFLAEHIVEVPLVEA"
     gene            1973..3682
                     /locus_tag="VHM48_06300"
     CDS             1973..3682
                     /locus_tag="VHM48_06300"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX2755053.1"
                     /translation="MTHRTAAQLLVECLAAEGCEYVFSVPGEETMGVLDALGVVAAAG
                     TGPRHVTTRHEQGAAFMADVYGRLTGNASVAMSTLGPGATNLITGVADAFLDRAPMVA
                     ITGQASSDKLHKEAHQVVDIVRMIEPVTKWNTRVERVEAIPEIVRKAFRIATLAKPGP
                     THIELPEDVAAAPVDDAARPLVPGKAYFPEPTDEAIAHAAEVIATSQRPLVLAGNGVL
                     RRHASPELRAFARGLHVPVAATFMGKGAMDDRSHLSLMAVGLQARDHVLTGFDRADLV
                     ICIGYDLVEYAPAHWNSDGRKRIVHIDTQPAEVDAQYQPEVELIGDIDGSLRRLLTAV
                     QPRGISGRDAGERHEAREALVHSDLRTALLAELAGSAGSREDALPITPQRAIADLRAA
                     LGPEDIVVSDVGAHKVWVARLYQAYEPNTVIISNGFAAMGISLPGAIAAKLVHPDRKV
                     VALCGDGGFLMNSQELETAKRIGANVTVVVWRDDGYGLIDWKQRNEFGRPFGVEFGNP
                     DFVDYARSFGIAGFRPTSAGDLLPTLRRALDVDGPSLVEVPIDYRENLRLTEHLGALA
                     GGA"
     gene            complement(3694..>4486)
                     /locus_tag="VHM48_06305"
     CDS             complement(3694..>4486)
                     /locus_tag="VHM48_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2755054.1"
                     /translation="TVWIPDELLWRPPDGQVQGWWEGVAMTGAVAAATSQVKVGTWIL
                     SALHRNPGITAKAVETLDEISGGRFVFGLGSGHAGRQAHAFGLPEDHVYGRFAEAVEI
                     IVPLLRHGRADFEGTFHAARDLEQRPVGPRPGRIPIMIGAKGPKMLRLAALHADIWSW
                     YVEERSDLEEFGPRLAALEAVCVEVGRDPATIGKSAGIVVEPTSVTGAADVLAAPVRG
                     SAEEIADALRAFGAGGFTQIEVLLWPRTLAALDAMAPVLELLDAD"
BASE COUNT          685 a         1631 c         1531 g          639 t
ORIGIN      
        1 ggacacccga agaattacgt ggtcctcgca gagaagccgg tcgaagaatc gtcgaagcag
       61 cgggccggcg tcgtcaacaa gtagagttgg caccgcgcgc tcggatccct ccgggcgggc
      121 cattcgtctc cagccgcgcg cgacccaggg atggtcgggg cagcggtcgg cggcggacct
      181 gagggaatgg cggccgccgc gtcggggcgt tcgccgggtc agacagggcg ggcatccgcg
      241 gtgggacgcg cgatggtcgc ccgtgatgag ggcgccagtg atccttcgta tcgtcagcgg
      301 acgggttccg gccgggcgcc tcgacgccgt cgtcgaggcc tatcggcgcg actacgtccc
      361 gatcgctcgc cagacggtcg ggctggatcg gttcctggtc gctgcgcgac tcgtgccgga
      421 cggtggtcac gaattcgctg cgatgacgat ctggacgtcg gtcgaggccg ccctcgccgc
      481 ctatgacgga aacctggctg ccgcccggac gctcgacgga caggggcatg gcgagacgct
      541 gacccgggtc gactactacg agatcgacgc gggcggagcc cgacgccgaa ccggcgcggc
      601 gacgcggctc cgcctgaccg ccgggacggt cgcccgtggg ctcgacgccg acatccagca
      661 ggagcttcgt cgccaccttc ccgaccttcc ggtcgaggcg atcgaggcat atgtcggtcg
      721 gcgggtcctc ggcggcgcgg tcgagatcgc cctcgtcacg acctggtcgg cggcgccggc
      781 gggcgtctcc ctggatgcgc ccttgtggcc gtccatcgcg gatcgctacg acacgttccg
      841 gatcgtggtc cacgacgtcc tgctcgacgg cgccggcgcc ggctgatcgc cacgcttctg
      901 cagcgccgtc cggcagatcc gttgtgccgc cgtcgagtgc cgtcgggcag cctgaggacg
      961 atgcaggcgc ggcccgacgc cgcccgatgg aggacctcat ggcaacgaca actcgacgtt
     1021 caacgtccaa ccgcaacggc tcgtccaacg gcaatggctc ggcgcgccag aaggtgtcag
     1081 cccagccccg gatgggccag accgcgcccg agctccagcg attcgggacg ctccgacagc
     1141 tgccgatcgc cctctccgcc gaggcccgcg ccgagagctg ccagctcctc aacgagatcc
     1201 tggccgacac gatggtcctt caggcgctgt acaagaagtc gcactggaac gtcgccggtc
     1261 cgaccttcta ccagcttcat ctcctcttcg acaagcattc cggcgaacag ctcgagctca
     1321 tcgacgagct tgcggagcgg gtccagatgc tcggtggcat cagcgtcggc gacccgcgtc
     1381 acgcggcgga gctgacctcg atcccgcgcc cgcccgacgg cgccgaggag gtgccggtga
     1441 tcctgtcgcg gctgctcgat gcccacgaga cgatcatcga gaaggtccgc gacgggctcg
     1501 acaagaccga ggagtcgaag gacctcggca cgaacgacct gctgatgggc gacgtcctcc
     1561 gccgccacga gatgcaggtc tggttcctcg ccgagcacat cgtcgaggtc ccgctcgtcg
     1621 aggcctgacc cgcggtccag ggcgcgtcgc cgcgtcgccc ggctcccgat tcttcttgcc
     1681 ccacgggcgt atccaacaag gtcgacccgt gacgagtgcg cgcagccact tcgatgcagc
     1741 gcggtcgggg tcgttcgcgg gccgaagcgc cgtcccgcga gcacgcgagc caacgccggc
     1801 ccccagccag ttcgaatagc cgaatatcca gggccgtccg ctggtgagcc accgcgcaca
     1861 ttcggctatt cgaaacgtgg tggccgactc actccgtgga caaacggcga atccgcctga
     1921 catctcgcgt cgcagcagtg ggcgctccgg ctgtggctgg tacgctcgcg aggtgactca
     1981 ccggaccgcc gcccagctgc tcgtcgaatg cctcgccgcg gagggctgcg agtatgtctt
     2041 ctcggtgccc ggcgaggaga caatgggggt cctcgacgcg ctgggcgttg tcgcagcagc
     2101 aggcaccgga ccccgtcacg tcacgacgcg ccatgagcag ggcgccgcat tcatggccga
     2161 cgtctacggc aggctgactg gcaacgcgtc cgtcgcgatg tcgaccctcg gcccgggcgc
     2221 gacgaacctg atcaccggcg tcgccgacgc tttcctcgat cgggcgccga tggtcgcgat
     2281 caccggccag gccagctccg acaagctcca caaagaggcc catcaggtcg tcgacatcgt
     2341 ccggatgatc gagccggtga cgaagtggaa tacccgcgtc gagcgcgtcg aggcgatccc
     2401 cgagatcgtc cgcaaggcct tccggatcgc gaccctcgcc aagccgggcc ccacccacat
     2461 cgagctgccg gaggacgtcg cggcggcacc cgtcgacgac gccgcccggc ccctcgttcc
     2521 cggcaaggcc tacttccccg agccgaccga cgaggcgatc gcccacgcgg cggaggtgat
     2581 cgcgacctcg cagcggccac tcgtcctggc cggcaacggc gtcctgcggc ggcatgcgtc
     2641 gccggagctg cgggcgttcg cgcgcggtct tcacgtcccg gtcgcggcga ccttcatggg
     2701 caagggcgcg atggacgatc gctcccacct gtcgctgatg gccgtcgggc tccaggcccg
     2761 cgaccacgtc ctgaccgggt tcgatcgggc ggacctcgtg atctgcatcg gctatgacct
     2821 cgtcgagtac gcgccggctc actggaattc cgacggccgc aagcggatcg tccacatcga
     2881 cacccaaccc gccgaggtcg acgcccagta ccagcccgag gtcgagctga tcggcgacat
     2941 cgacggctcg ctccggcggc tcctcaccgc ggtccagccc cgtggaatca gtggccgcga
     3001 cgccggggag cggcacgaag cgcgcgaggc cctcgtccac tcggacctca ggaccgccct
     3061 cctggccgag ctcgcgggca gcgccggatc gcgcgaggac gccctgccga tcaccccgca
     3121 gcgggcgatc gccgatctcc gcgcggccct cggtccggag gacatcgtcg tgtccgacgt
     3181 cggcgcccac aaggtctggg tcgcccggct ttaccaggca tacgagccga acaccgtgat
     3241 catctcgaac gggttcgcgg cgatgggaat cagcctgccg ggcgcgatcg cggcaaagct
     3301 cgtccatccc gaccggaagg tcgtcgccct gtgcggcgac ggcggcttcc tgatgaacag
     3361 ccaggagctc gagaccgcca agcggatcgg ggcgaacgtg acggtcgtcg tctggcgcga
     3421 cgacggctac ggcctgatcg actggaagca gcgcaacgag ttcgggcggc cgttcggggt
     3481 cgagttcggc aaccccgact tcgtcgacta cgcccgctcg ttcgggatcg ccgggttccg
     3541 gccgacctcc gcgggggacc tgctgccgac gctgcggcga gccctggacg tcgacggccc
     3601 gtcgctcgtc gaggtcccga tcgactaccg cgagaacctc cgcctgaccg agcacctcgg
     3661 cgcgctcgcg ggcggcgcct gaccgatccg ggatcagtcc gcgtcgagga gctcgagcac
     3721 cggcgccatc gcgtcgagcg cggccagcgt ccgtggccag agcagaacct cgatctgcgt
     3781 gaagccgccg gcgccgaacg cccggagcgc atcggcgatc tcctcggccg agccgcgcac
     3841 cggcgccgcg agcacgtccg cggctccagt gaccgaggtc ggctcgacca cgatgccggc
     3901 cgacttcccg atcgtcgcag gatcccggcc gacctcgacg cagactgcct cgagcgccgc
     3961 gagtcgcggc ccgaactcct cgaggtcgct ccgttcctcg acgtaccagc tccagatatc
     4021 ggcatggagg gcggccaggc ggagcatctt cggacccttg gcgccgatca tgatcgggat
     4081 ccggccgggg cgcggtccga cgggccgctg ctcgaggtcg cgcgccgcgt ggaaggtccc
     4141 ctcgaagtcc gcgcggccat ggcgcaggag cggcacgatg atctcgaccg cctccgcgaa
     4201 gcggccgtag acatggtcct cgggcagccc gaaggcatgc gcctggcgac cggcatgccc
     4261 ggagccgagg ccgaacacga agcggccgcc gctgatctca tcgagcgtct cgaccgcctt
     4321 cgccgtgatc ccggggttcc ggtggagcgc ggacaggatc cacgtgccga ccttcacctg
     4381 tgaggtcgcg gcggccactg cgccggtcat cgcgacgccc tcccaccagc cctgcacctg
     4441 accgtcgggc ggacgccaca acagttcgtc cggtatccag acggtg
//
LOCUS       DATAIW010000195         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183336, whole genome shotgun sequence.
ACCESSION   DATAIW010000195 DATAIW010000000
VERSION     DATAIW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183336"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VHM48_06310"
     CDS             <1..263
                     /locus_tag="VHM48_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2755055.1"
                     /translation="LELVARHTIDVLVSDVVMPTMTGPELAREVLNRYPRVGVVLVSG
                     YTAESLELTELMSRGAQFVDKPFSATDLLSAVDRALESRVAP"
     gene            327..1436
                     /locus_tag="VHM48_06315"
     CDS             327..1436
                     /locus_tag="VHM48_06315"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2755056.1"
                     /translation="MPTLDTIRQAPKALLHDHLDGGLRPATVVDLAREFGYDGLPTDD
                     VDELAAWIRRGADRKSLELYLETFACTVGVMQQRAAIVRVAAECAEDLAADGVAYAEV
                     RYAPELSTERGLTLDEVVEANLEGFRIGMDRAAEAGHPISMKMIVTAMRQAARSVEVA
                     ACALRHRDEGVVGFDIAGPEDGYRPTRHLDAFDLVRRENFHITIHAGESFGLPSIWEA
                     LQFCGAERLGHGVRIVDDIHVRDDGSVELGRLATFVRDRRVPLEMCPTSNVHTGAAAT
                     IAEHPIDLLRRLRFRVTLNTDNRLMSGVSLSSEFAALDAAFGVGLGEMEWLTINAMKS
                     AFAPFDERLRLINEVVKPGYAHLRAIESRVVGTSG"
     gene            1672..2964
                     /locus_tag="VHM48_06320"
     CDS             1672..2964
                     /locus_tag="VHM48_06320"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX2755057.1"
                     /translation="MSDSSLVLPEPTGDAPAVANAMASAALAPTTLVLEAPAPVGAVT
                     TTTASEAVPINAADRARLDSMVESYLDAVSNLDPHGTEFGDKVKDIGKLGDDDIRASA
                     SVSNRLLDKPLAAMQNGGLTETSSVGRSLLSLRRQVEDLDPAKQGDLLSPRKLLGLLP
                     FGAGDKLRHYFDKYRSSQHQLEGIIKSLDHGQQELERDNDSIEQEKVNLWAVMGRLRQ
                     YAYLAAALDDALAARIATIEPTDPERAKVLSEDMLFSVRQKNQDLLTQLAVSVQGYLA
                     LDVIRRNNLELIKGVQRATTTTISALRTAVIVAQAMADQKLVLDQITALNTTTSNLIE
                     STSEMLHQQSSAINEQAASATVDLARLQAAFTNIYATMDEIDTFKVQALDTMQKTVTA
                     LTTEITKSQAYLDRVRTDDTPGGPIDALGPGSIGADPG"
     gene            complement(3461..>3976)
                     /locus_tag="VHM48_06325"
     CDS             complement(3461..>3976)
                     /locus_tag="VHM48_06325"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755058.1"
                     /translation="DRLVGIKSREIYEAPAATILHAAHGALEGLTLTKDTLRFNRLVA
                     SELAQLTYDGLWFSALHRDLRGYVASAQRVVSGEVRARLDHGSTVVVGRRSPMSLYDK
                     NLATYDTGDAFDHAAAVGFISIFGLPLRVEAARHGAVGAHYGAAWTWTDPLLADLPTT
                     VTDPAEAVAAG"
BASE COUNT          638 a         1425 c         1350 g          563 t
ORIGIN      
        1 ccctcgagct cgtcgcgcgc cacacgatcg acgtcctcgt cagcgacgtg gtaatgccga
       61 cgatgacggg acccgagctc gcccgcgaag tgctgaaccg gtatccgcgg gtcggcgtgg
      121 tcctcgtctc gggctacacg gccgagtcgc tggaattgac cgagctcatg agccggggtg
      181 cgcagttcgt cgacaagccg ttctcggcga ccgacctgtt gtcggcggtc gatcgggcgc
      241 tggagtcgcg ggtcgcgccc tgagtccgtc ggccggcgcg cggtcgtacc atcggtgggc
      301 acccgatcga gaagacgaga cgaacgatgc ccaccctcga cacgatccgc caggctccca
      361 aagccctcct ccacgaccat ctcgacggcg ggttgcggcc cgcgaccgtc gtcgacctcg
      421 cccgcgagtt cggctatgac gggctcccca cggatgacgt cgacgagctc gccgcgtgga
      481 tccggcgtgg cgccgaccgc aagtcgctcg agctctacct cgagaccttc gcctgcacgg
      541 tcggggtcat gcagcagcgc gccgcgatcg tccgggtcgc cgccgagtgc gccgaggacc
      601 tcgccgccga cggcgtcgcg tacgccgagg tccgctacgc cccggagctc tcgaccgagc
      661 gcgggctgac gctcgacgag gtcgtcgagg ccaacctcga ggggttccgg atcgggatgg
      721 accgggccgc cgaggccggc cacccgatca gcatgaagat gatcgtcacc gcgatgcgcc
      781 aggccgcacg ctcggtcgag gtcgcggcct gtgccctccg ccaccgcgac gagggcgtcg
      841 tcggcttcga catcgccggg cccgaggacg gctatcgccc gacccgccac ctcgacgcgt
      901 tcgatctcgt ccgccgcgag aacttccaca tcacgatcca cgccggcgaa tcgttcgggc
      961 tgccgtcgat ctgggaggcg ctccagttct gcggcgcgga gcggctcggc cacggtgtcc
     1021 ggatcgtcga cgacatccac gtccgcgacg atggctcggt cgagctcggg cgactggcca
     1081 cattcgtccg ggaccggcgc gtaccgctcg agatgtgccc gacctcgaac gtccacaccg
     1141 gggcagcggc gacgatcgcg gagcacccga tcgacctcct ccgccggctc cggttccggg
     1201 tcacgctcaa caccgacaac cggctgatga gcggcgtctc gctctcgtcc gagttcgccg
     1261 ccctcgatgc ggcgttcggg gtcggcctcg gcgagatgga gtggctgacg attaacgcga
     1321 tgaagagcgc gttcgcgccg ttcgacgagc gcctccgcct gatcaacgag gtcgtgaagc
     1381 cgggctacgc ccacctccgc gcgatcgaga gccgggtcgt cggcacgagc ggttgaggcg
     1441 agcggccggc tgggtcccgg ttcggacgtc cggcgaccga accggcgatg gagcggcgag
     1501 ggcagggcga gtccacgtac cgagatatcg cttgcggggc ggcgcaacac gtgatctgat
     1561 tcgacccgag ccgggacggg cggatggagc ccactctcaa tgcgcacggc ccagtgcggc
     1621 tccgacctga cgaggagccc gatgcgcacc gccgcctgaa ggagcccgtc gatgagcgac
     1681 tcatccctcg tcctgccgga gccgaccggc gatgcgccgg cggtcgcgaa tgcgatggcc
     1741 tccgctgcgc tcgccccgac gacgctcgtc ctcgaggcgc cggcgccggt cggtgccgtc
     1801 acgacgacga ccgccagcga ggcggtgccg atcaatgccg ccgatcgggc caggctcgac
     1861 tcgatggtcg agagctatct cgacgccgtc tcgaacctcg atccccacgg caccgagttc
     1921 ggcgacaagg tcaaggacat cggcaagctc ggcgacgacg acatccgggc gtcggcgtcg
     1981 gtgtcgaacc gcctcctcga caagccactg gccgcgatgc agaacggcgg cctgaccgag
     2041 acgtcgtcgg tcggccggtc gctcctgagc ctccgtcgcc aggtcgagga tctcgatccg
     2101 gcgaagcagg gcgacctgct cagcccgcgc aagctcctcg gtttgctgcc gttcggcgcc
     2161 ggcgacaagc tccgccacta cttcgacaag taccgctcga gccagcacca gctcgagggg
     2221 atcatcaagt cgctcgatca cggccagcag gagctcgagc gggacaacga ctcgatcgag
     2281 caggagaagg tcaacctctg ggcggtcatg ggtcgcctcc gccagtacgc ctatctggcg
     2341 gcggcgctcg acgacgccct cgccgccagg atcgcgacga tcgagccgac cgatcccgag
     2401 cgggcgaagg tcctcagcga ggacatgctc ttctccgttc gccagaagaa ccaggacctg
     2461 ctgacccagc tcgccgtcag cgtccagggc tatctggccc tcgacgtgat ccgccgcaac
     2521 aacctcgagc tgatcaaggg cgtccagcgc gcgacgacga cgacgatctc ggcgctccgg
     2581 accgcggtga tcgtggccca ggcgatggcc gaccagaagc tcgtcctcga ccagatcacc
     2641 gccctgaaca cgacgacctc gaacctgatc gagtcgacgt ccgagatgct ccaccagcag
     2701 tcgagcgcga tcaacgagca ggccgcctcg gcgacggtcg atctcgcccg gctccaggcc
     2761 gcgttcacga acatctacgc gacgatggac gagatcgaca cgttcaaggt ccaggccctc
     2821 gacacgatgc agaagaccgt caccgcgctc acgaccgaga tcacgaagtc gcaggcttat
     2881 ctcgaccgcg ttcgaaccga cgacacgccg ggcggcccga tcgatgcgct tggcccgggt
     2941 tcgatcgggg ccgatccggg ctgaccgatt ggattgacgg cgccgacgca catgccgttt
     3001 gtccggcgtg gcgcgagccg cagttccgct ggtcttcctg ccagcagttc cgctggtctt
     3061 cctgccatct gttcgcgagg cgcgcaacgc atccggcctt gcgagcccgt tcctgccagg
     3121 ggattgccca ggccgccaag tgccaggtca tttcctgacc aacggcgccg tcgcggccac
     3181 ctggcaggca ctcgaccgcc gctcgacgga ccgcgccgag tctcaggctg gcagacgcgc
     3241 gccgaggacg acatctgacc tgcggttgat ggcacgcggc gcctcagtga gcaccacgag
     3301 cccaaatgtc aggaagggcg cccggtggca gaacggcgcc cggtggcaga acggcgcccg
     3361 gtggcagaag ggcgcccggt ggcagaacgg cgcccggtgg ctgcggcgcg cgcgggcacg
     3421 gccaggggat cggcgccccc caacgcgacc ggtcggttcc tcagcccgcg gcgaccgcct
     3481 ccgccggatc ggtgacggtc gtcggcaggt ccgcgaggag cggatcggtc cacgtccagg
     3541 ccgcgccgta gtgggcgccg accgccccgt ggcgggcggc ctcgacccgc agcggcaggc
     3601 cgaagatcga gatgaacccg accgcagcgg cgtgatcgaa ggcgtcaccg gtgtcgtagg
     3661 tcgcgagatt cttgtcgtac agcgacatcg gtgagcgccg ccccacgacc accgtcgagc
     3721 catgatcgag ccgggcgcgg acctcgccac tgaccacgcg ctgagccgac gccacgtagc
     3781 cgcgcaggtc acggtgcagc gccgagaacc acagcccgtc gtacgtcaac tgggccagtt
     3841 cgctcgcgac caggcggttg aagcggaggg tgtccttggt gagcgtcagc ccctcgagcg
     3901 cgccgtgtgc cgcgtggagg atcgtcgccg caggcgcctc gtagatctcg cggctcttga
     3961 tcccgacgag gcggtc
//
LOCUS       DATAIW010000196         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_99537, whole genome shotgun sequence.
ACCESSION   DATAIW010000196 DATAIW010000000
VERSION     DATAIW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99537"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHM48_06330"
     CDS             <1..392
                     /locus_tag="VHM48_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX2755059.1"
                     /translation="LSDDFPIRLTTGRRLDSYNTGVQTGGYTSPLRRGESLDISPEDA
                     ADYGLADGERVRVVSRRGHVETPIRIDESLRPGLTFMTLHFQDDVATNLLTIDATDPK
                     SGTAEFKATAIRIERLAQPVSPAGAGR"
     gene            389..610
                     /locus_tag="VHM48_06335"
     CDS             389..610
                     /locus_tag="VHM48_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755060.1"
                     /translation="MNGSPDAPPPDPLVRRLAGACLLGLSLAILLVAGPIGLASSSST
                     ALAATVAGAALGASGLTLLLRPEGRGQGS"
     gene            620..1048
                     /locus_tag="VHM48_06340"
     CDS             620..1048
                     /locus_tag="VHM48_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2755061.1"
                     /translation="MTIPARLNIVTIGVADVARSVRFYEALGWQRCSSSMDEIAWFRT
                     ADSYLGIFGWQDLADDANLLEPTRGSFGGITLAINIESSDAVDAALAEAAAAGGTILK
                     PGTELPFGYGGYFADPDGHPWEVCYNPSFPIGPDGRITID"
     gene            1059..1445
                     /locus_tag="VHM48_06345"
     CDS             1059..1445
                     /locus_tag="VHM48_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX2755062.1"
                     /translation="MKIVATADAPSGNAAGRFSYSQAVVAGGLVFVAGQGPFDPATGA
                     IVGTTIQEQTRQALANVRAILEAAGSSMDRVVNVTFVLTRPEDFAGMNEEWQRWFPAN
                     PPARSGARHPLQVDNLLISMAIIAEA"
     gene            1442..1918
                     /locus_tag="VHM48_06350"
     CDS             1442..1918
                     /locus_tag="VHM48_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755063.1"
                     /translation="MSIEHRATRTVDQRPEVVHDRLLELAARVRDETPPVEPGTQIST
                     LLGMSGDLGIEVADRGPRRIELRTTQGRIRGEGVAELAATADGRTTITLDGAVKAQGF
                     AANLMLGVALKAMPSLEKDIVDGLEKGLDELAVELAKSDADWNAADWRPSQLPARA"
BASE COUNT          307 a          785 c          731 g          294 t
ORIGIN      
        1 agctcagcga cgacttcccg atccgcctca cgaccggccg ccgcctcgac tcgtacaaca
       61 cgggcgtcca gaccggcggc tacacgtcgc cgttgcggcg cggcgagtcg ctcgacatct
      121 cgccggagga cgccgccgac tacgggctgg ccgacggtga gcgcgtccgg gtcgtgtcgc
      181 gacgcggcca tgtggagacc ccgatccgga tcgacgagtc gctccggccg ggcctgacgt
      241 tcatgaccct ccatttccag gacgacgtcg cgacgaacct cctcacgatc gacgcgaccg
      301 acccgaaatc cgggaccgcc gagttcaagg cgaccgcgat ccggatcgag cgccttgccc
      361 agccggtgtc accggccggc gccggacgat gaacgggagt ccggacgcgc cgccacccga
      421 tccgctcgtt cgtcgcctcg ccggggcgtg cctgctcggc ctgtccctcg ccatcctgct
      481 cgtcgccggt ccgatcggtc tggcatcgag ctcctccacc gcgctcgcgg cgaccgtcgc
      541 gggcgcagcg ctcggagcca gcggcctgac ccttctcctc cggccggagg gtcgcggcca
      601 ggggtcgtag actgccggga tgaccattcc cgctcgcctc aacatcgtca cgatcggcgt
      661 cgccgacgtc gcccgatccg tccgcttcta cgaggccctc ggctggcaac ggtgctcgtc
      721 ctcgatggat gagatcgcct ggttccggac cgccgacagc tatctcggga tcttcggctg
      781 gcaggacctc gccgatgacg ccaacctcct cgagccgacc cgcggctcgt tcggcgggat
      841 caccctcgcg atcaacatcg agtcgtccga cgcggtcgac gccgcgctgg ccgaagctgc
      901 ggctgccggc ggcacgatcc tgaaacccgg cacggagctc cccttcggct atggcggcta
      961 cttcgccgat ccggacggcc atccctggga ggtctgctac aacccatcgt tcccgatcgg
     1021 tccggacggc cggatcacga tcgactgagg agctcaccgt gaagatcgtc gcgaccgccg
     1081 acgccccgag tggcaacgcc gccgggcgct tctcgtacag ccaggccgtg gtggcgggcg
     1141 gcctggtctt cgtcgccggc cagggcccgt tcgatcctgc gaccggcgcg atcgtcggaa
     1201 cgacgatcca ggagcagacc cgccaggccc tggccaacgt gcgggcgatc ctcgaggcgg
     1261 ccggcagctc gatggaccgc gtcgtcaacg tcaccttcgt cctgacccgg cccgaggact
     1321 tcgccgggat gaacgaggag tggcagcgct ggttcccggc gaacccgccg gcccgctccg
     1381 gcgcccgcca cccgctccag gtcgacaacc tcctgatctc gatggcgatc atcgccgagg
     1441 catgagtatc gagcatcgcg ccacccggac cgtcgaccag cgcccggagg tcgtccacga
     1501 ccggctcctc gagctcgccg cgcgcgtccg cgacgagacg ccgcccgtcg agcccggcac
     1561 ccagatctcg acgttgctcg ggatgtcggg cgacctgggg atcgaggtcg ccgaccgcgg
     1621 gccgcgacgg atcgagctcc ggacgaccca ggggcggatc cgcggcgaag gggtggcgga
     1681 gctcgccgcg acggccgacg gccggacgac gatcacgctc gacggcgcgg tcaaggccca
     1741 ggggttcgcg gccaacctga tgctcggggt cgcgctcaag gcgatgccga gcctcgagaa
     1801 ggacatcgtc gacggactgg agaagggcct cgacgagctg gctgtcgagc tcgccaagag
     1861 cgacgcggac tggaacgccg cggactggcg gccgtcgcag ctgccggcgc gcgcctgacc
     1921 gatcgccggc cggcgccgcc gggcgccggg cgaccggacg ccggccgcgc cggagaaacc
     1981 agggtcgagc gagcctacgg tcgggcgatc gtgatcgacg tgccggtcga atcgccgtcc
     2041 gggtcgtggt tggcggacgg atcgtaggtc gcggttccgc tcgtgaccga cgtcacggtg
     2101 aaggtcgcgg cctggac
//
LOCUS       DATAIW010000197         5931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_242811, whole genome shotgun sequence.
ACCESSION   DATAIW010000197 DATAIW010000000
VERSION     DATAIW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242811"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            492..692
                     /gene="rpmF"
                     /locus_tag="VHM48_06355"
     CDS             492..692
                     /gene="rpmF"
                     /locus_tag="VHM48_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2755064.1"
                     /translation="MGVPKRRVSHARQGERRAHLALSVPELVECDHCHEKKLPHHVCP
                     NCGWYQGRQAVELKQTATDTSS"
     gene            694..1860
                     /gene="plsX"
                     /locus_tag="VHM48_06360"
     CDS             694..1860
                     /gene="plsX"
                     /locus_tag="VHM48_06360"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX2755065.1"
                     /translation="MVAALDRPFPSTAGSALDDGRGVTIAVDAMGGDHGPSEVVPGAL
                     DHALANPQDRVILVGDEPTLRRIAGALPPNVSIVHASEVVGMDEHPAMALREKKDSSI
                     LVATDLVKRGEADAVVTAGHSGAGMGAAVLRLGRLRGVDRPALAVPLTTEAGTLVLLD
                     IGANTDSSAENLAQYARMGSIFSERVLGVTNPRVALLSIGEEKGKGDLRIQQATELLD
                     QTDLNFVGNIEGKDLTRHQADVVVCDAVLGNVVIKFFEGLSTYIFDMLRREFRGSLRG
                     KVAGLLMRPGIARIRAVFDYERVGGSPLLGVKGTVIITHGRAKRRMIGYGVGVAATMA
                     RTRVPELIAEALRPAPDTSEAEAPAGSSTGDAPAGPPSAGPPSTGAAAAVESAS"
     gene            1857..2171
                     /locus_tag="VHM48_06365"
     CDS             1857..2171
                     /locus_tag="VHM48_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2755066.1"
                     /translation="MSGPELTIGRGVIAEMVRLAAFEIPGVARVSRGGPAWRTALGGP
                     PVVTRLSEDGVRVRVWIIARPGQQLVTLAGDVRTAVGAAVERLLGLRLESVTVTVDGV
                     GG"
     gene            2155..2592
                     /gene="nusB"
                     /locus_tag="VHM48_06370"
     CDS             2155..2592
                     /gene="nusB"
                     /locus_tag="VHM48_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2755067.1"
                     /translation="MASEAETGADRIRALRAGRRLALAAVFEAEFGQRTADAVLERHL
                     AETEGDPRAADLARSLVANVIAHRDTIDARIVTTAPQYPVGGLARMDRALLRCAIGEV
                     LHSPTPPRVAIAEWVELARTYSGDPMRRLMNGVLGRIANTVDG"
     gene            2624..2860
                     /gene="acpP"
                     /locus_tag="VHM48_06375"
     CDS             2624..2860
                     /gene="acpP"
                     /locus_tag="VHM48_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2755068.1"
                     /translation="MSTTYERLKKLVVEQLGVEEDEVKPEASFVDDLNADSLDLVELI
                     MSLEEEFGTEISDEDAEKIRTVQDAVDYIDERST"
     gene            2885..3634
                     /gene="rnc"
                     /locus_tag="VHM48_06380"
     CDS             2885..3634
                     /gene="rnc"
                     /locus_tag="VHM48_06380"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX2755069.1"
                     /translation="MRPADALAERLGLPVGDSDLREQALVHTSWLHEHPDAAAGHNER
                     LEFLGDAVVNLAISEALFAAHPADDEGMLSARRASIVSTVGLARLAGRIDLGPSLFLG
                     EGEAQRGGRRRPSILASALEALAGALYLDLGYDLTRDWLLAIAAPEISLDAPLSSLKS
                     PKSRLQEYTQRRTGSRPEYHVVDASGPDHEKLFQVEVLVDGRPLGVGVGPSRRVAETA
                     AAQRAIETLRTEVAERPLGSSPATTPAETAQ"
     gene            3631..>5931
                     /locus_tag="VHM48_06385"
     CDS             3631..>5931
                     /locus_tag="VHM48_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755070.1"
                     /translation="MSAPARLLALRLQGFKSFAERTIVEFGPGISAIVGPNGSGKSNL
                     ADALRWSLGEQGRALRSRKSEDVIFAGSEKRSALGMADVTLVLDNADGLLPVDFSVLE
                     LGRRLYRSGENDYLLNKQRIRLRDLVDLLDGAHLADNAFLFIGQGMVDQALALRPEER
                     RPLFEEVAGVRRHERRRRKAEEQLAESETNLARVEDILAELRPQARRLAAQAEQQTTR
                     LSAGEELASALLAAAHVRWHEAAGQAAAAATRVERERAEADRAMTDLQAAEETAAAIG
                     AELGVRAATERERLASHETARGAVTELRLREARLAGDLEALARDRTRLRTERETAEAS
                     LAVQRRGLAIAIPPRDHDLDASLAEAERLLAEANGELAALRTASRARGEEAAAIRRAE
                     AARQSEAETARRRLADGLRRLDAEQAAAAEVAARRAELEGRHAGAGAALEGALTASAG
                     ATTTRETTRASAAEADGRRHSAADRAGAAGATRVAQEARLESLDGRIREDDERGIARA
                     AKRRGGRRLDEELVVEPTMRAAVESTLDELARAYLIDEAAAGSLAAERGMLVIRERAT
                     GPGPQSTSDVVTRRFLERLAAAGGTPLATAIRRDPGGTVTRILARTVVVDDLATALDL
                     QAHLPAGWAIATRDGAAVVTELAVRLGRGDAPLERRAEADRLRSDVARLRAEADAAAT
                     ESAAAERAAATTRTALEAATTEEARADADRRRAEEAERGAARELEAVAREAAWHAAQV
                     ERLAADVERARESLAAHESTGLWGNTT"
BASE COUNT          823 a         2185 c         2153 g          770 t
ORIGIN      
        1 ccgacaggac tgtccggggc tgtgcatcgt ctgcggcctg ccgctcgacg agggcgacca
       61 cgatcacccc gacgacgatg tcgaccctcg gctggaggcg ctccgggggt tcgtcgcgga
      121 ctgaccggcg ccagctcgga acccacgcgg ccccgcgcga cgagcaggct tcgacgtccg
      181 ttgcacgcca ggcgggcatt gcgacgaacg ggaggccgct cagcctctga tgtgcccgct
      241 cggtgggcag gatggcgaag gaggaaccgt tttcggggcc ggatcagccg ttgcttcgtc
      301 gtaacgctcg ccacgcgtgc aatcgggtcg ggaggcggat tgcgagggcc gctgagcccg
      361 caccaactcg cgcgggaacg gcgcttccgg tacactcggc catcggccct gccagtccgg
      421 ctgggcgtct cggcgcgtcc cacatcccgg ccgcccgacc cgaacgctga catcgaagga
      481 gaccacggac cgtgggagtt cccaagcgac gcgtgtcgca tgcccgccag ggtgagcgtc
      541 gcgcgcacct ggcgctgagc gtgccggagc tcgtggagtg cgatcactgc cacgagaaga
      601 agctgccgca ccacgtctgc ccgaactgcg gctggtatca gggccgccag gccgtcgagc
      661 tcaagcagac cgcgaccgac acctcctcct gaggtggtcg ccgcgctcga tcgcccgttt
      721 ccgtcgaccg ccggctccgc cctcgatgac gggcgtgggg tgacgatcgc ggtcgacgcg
      781 atgggtggcg accacggacc gagcgaggtc gttccgggag cgctcgatca cgctctggcg
      841 aatccccagg atcgggtgat cctcgtcggc gacgagccga cgctccgtcg catcgccggc
      901 gctctgccgc ccaacgtctc gatcgttcac gcgtcggagg tcgtcgggat ggacgagcac
      961 ccggcgatgg ccttgcgcga gaagaaggac tcctcgattc tcgtcgccac cgacctcgtg
     1021 aagcggggcg aggccgacgc cgtcgtcacc gccggtcact ccggcgccgg gatgggcgcc
     1081 gccgtcctgc gtctcggccg gctgcgcggc gtcgaccggc cggcgctcgc cgtgccgctc
     1141 acgaccgagg ccgggacgct cgtcctcctg gacatcgggg cgaacaccga ctcgagcgcc
     1201 gagaacctcg cccagtacgc ccggatgggc tcgatcttca gcgagcgcgt cctcggcgtg
     1261 acgaacccgc gcgtcgcgct cctgtcgatc ggcgaggaga agggcaaggg cgacctccgg
     1321 atccagcagg cgaccgagct cctcgaccag accgacctga acttcgtcgg caatatcgag
     1381 ggcaaggacc tgacccgcca ccaggccgac gtcgtcgtct gcgacgcggt cctcggcaac
     1441 gtggtgatca agttcttcga gggcctctcg acgtacatct tcgacatgct ccggcgcgag
     1501 ttccgcggct ccctgcgcgg caaggtcgcc ggcctcctga tgcggccggg gatcgcccgg
     1561 atccgggccg tgttcgacta cgagcgggtc ggcggatcgc cgctcctcgg ggtcaagggg
     1621 accgtgatca tcacccacgg tcgggcgaag cggcggatga tcggctacgg cgtcggcgtc
     1681 gccgcgacga tggcccggac ccgcgttccc gagctcatcg ccgaggccct tcgcccggcg
     1741 cccgacacga gcgaggcgga ggcgccggcc gggtcgtcga ccggggacgc tcccgccgga
     1801 ccgccgtcgg ccgggccacc ctcgaccgga gccgccgcgg ccgtggagtc cgcctcatga
     1861 gcgggcccga gctcacgatc gggcgcggcg tgatcgccga gatggttcgc ctggccgcct
     1921 tcgagatccc cggtgtggcc cgggtctcgc gcggcgggcc cgcctggcgg accgctctcg
     1981 gcgggccgcc ggtcgtgacg cgcctgtcgg aagatggcgt tcgcgtccgt gtctggatca
     2041 tcgcccggcc tgggcagcag ctcgtcaccc tcgccggcga cgtgcggacc gcggtcggtg
     2101 ccgccgtcga gcgcctgctc ggactccgcc tggagtcggt caccgtgacc gtcgatggcg
     2161 tcggaggctg agactggcgc tgaccggatc cgggccctcc gggcgggccg ccggctggcg
     2221 ctcgccgcgg tgttcgaggc ggagttcggg cagcggaccg ccgacgccgt cctcgagcgc
     2281 cacctcgccg agaccgaggg cgatccacgg gcggccgacc ttgcccgcag cctcgttgcc
     2341 aacgtcatcg cccaccgtga cacgatcgat gcccggatcg tcacgaccgc gccccagtat
     2401 ccggtcggcg gtctggcccg gatggaccgc gccctgctcc gttgcgccat cggtgaggtg
     2461 ctacactcgc cgacgccgcc ccgggtggcg attgccgaat gggtcgagct cgcgcgcacc
     2521 tacagtggag acccgatgcg gcgcctgatg aatggcgttc tcgggcggat cgccaacacg
     2581 gtcgacgggt agtcggcttc cagctccgag gagggagttc ccagtgtcca caacgtacga
     2641 gcgactgaag aagctcgtgg tcgagcagct tggcgtcgaa gaagacgagg tcaagcccga
     2701 agcgtcgttc gtcgacgacc tgaatgcgga ttccctcgat cttgtcgagc tcatcatgag
     2761 cctcgaggaa gagttcggga ccgagatctc cgacgaggac gcggagaaga tccgcaccgt
     2821 ccaggacgcg gtcgactaca tcgacgagcg gtccacctag aaccgcgcca tcgacccggg
     2881 agccgtgcga cccgccgacg cgcttgcgga acggctgggc ctccccgtcg gcgattccga
     2941 cctccgcgag caggccctcg tccacaccag ctggctgcac gagcatcctg acgcggccgc
     3001 cggccacaac gagcgcctcg agttcctcgg cgacgccgtc gtcaacctcg cgatctcgga
     3061 agccctgttc gccgcccatc cggccgacga cgaggggatg ctcagcgccc gccgggcgtc
     3121 gatcgtctcg accgtcgggc tcgcccggct cgccggccgg atcgacctcg ggccatccct
     3181 cttcctcggc gagggcgagg cccagcgcgg tggtcggcgc cggccctcga tcctcgcctc
     3241 ggcgctcgaa gcgctggccg gcgccctcta tctcgatctc ggctacgacc tgacccgcga
     3301 ctggctgctg gccatcgccg ctcccgagat cagcctcgac gcgccgctct cgagcctgaa
     3361 gagtccgaag agcaggctcc aggaatacac ccagcggcga accggctcgc ggcccgagta
     3421 ccacgtcgtc gacgcgtccg ggccggacca cgagaagctg ttccaggtcg aggtcctcgt
     3481 cgatgggcgg ccgctcggcg tcggggtcgg gccgtcgcgc cgcgtcgccg agactgcagc
     3541 cgcccaacgg gccatcgaga ccctccggac cgaggtcgcc gaacgaccgc tggggtcgag
     3601 cccggccacg acgcccgccg agacggcgca gtgagcgctc ccgcccgact ccttgctctc
     3661 cgcctccagg gcttcaagtc gttcgccgag cggacgatcg tcgagttcgg gccggggatc
     3721 agcgcgatcg tggggcccaa cggctcgggc aagagcaacc tagccgacgc cctccgctgg
     3781 tcgctcggcg agcagggccg ggccctccgc agccgcaagt ccgaggacgt gatcttcgcc
     3841 ggctccgaga agcgttcggc cctcggcatg gccgacgtga cgctggtcct cgacaacgct
     3901 gacgggttgc tgcctgtcga cttcagcgtc ctcgagctcg gtcggcggtt gtaccggagc
     3961 ggcgagaacg actacctgct gaacaagcag cggatccggc tgcgcgatct cgtcgacctg
     4021 ctcgacggcg cccacctggc ggacaacgcg ttcctgttca tcggccaggg gatggtcgac
     4081 caggccctcg ccctgcggcc cgaggagcgg cggccgctgt tcgaggaggt cgccggggtc
     4141 cggcgccacg agcgacgccg gcgcaaggcc gaggaacaac tcgctgaatc ggagacgaac
     4201 ctcgcccggg tcgaggacat cctcgccgag ctgcgaccgc aggcccgcag gctggccgcc
     4261 caggccgagc agcagacgac gcggctgtcg gccggcgagg agctggcctc ggcgctcctt
     4321 gccgccgccc acgtccgttg gcacgaggcg gccgggcagg ctgccgccgc cgcgactcgg
     4381 gtcgagcgcg agcgggcgga ggccgatcgg gcgatgaccg acctgcaggc cgccgaggag
     4441 accgcggccg cgatcggcgc cgagctgggg gtgcgcgcgg ccaccgagcg ggagcggctg
     4501 gcgagccacg aaaccgcccg cggcgccgtc acggagctcc gtctccgcga ggctcgcctg
     4561 gccggcgacc tcgaggcgct cgcccgcgac cggacgcgac tccggactga gcgcgagacc
     4621 gccgaagcgt cgctcgccgt ccagcgtcgc ggtctcgcga tcgcgatccc gcctcgcgac
     4681 catgatctcg acgcgtcgct ggcggaggcg gagcggctgc tggccgaggc gaacggcgag
     4741 ctggccgcgc tccggacggc cagtcgggcc cgcggcgagg aggcagccgc gatccgccga
     4801 gccgaggctg cccgacagtc cgaggcggag accgctcgcc gccggctggc cgacggtctg
     4861 cgccggcttg acgccgagca ggcggccgcg gccgaagttg cggctcgccg ggcagagctc
     4921 gaggggcgcc acgccggggc cggtgccgcg ctggaaggcg cgctgaccgc gtccgccggc
     4981 gcgaccacga cccgggagac gacccgggcg tcggccgccg aggccgacgg tcgtcggcac
     5041 agcgcggccg accgggccgg tgccgccggc gcgacccggg tggcccagga ggcccgcctc
     5101 gagtcgctcg atggccggat ccgcgaggat gacgagcgcg ggatcgcccg ggccgccaag
     5161 cgtcgcggcg gtcggaggct cgacgaggag ctggtcgtgg agccaacgat gcgagccgcc
     5221 gtcgaatcta cgctcgacga gctcgcccgg gcgtatctca tcgacgaagc ggccgccggg
     5281 tcgctcgcgg ccgagcgggg gatgctcgtc atccgcgagc gggcgaccgg tccgggcccg
     5341 cagtcgacgt ccgatgtggt cacgcgacgt ttcctcgagc gcctcgccgc cgcgggcgga
     5401 acgccgctcg cgacggccat tcggcgcgat cccggcggga cggtgacgcg gatcctggcc
     5461 cggaccgtcg tggtcgacga tcttgccact gcgctcgatc tccaggcaca cctgccggcc
     5521 ggctgggcga tcgcgacacg ggacggagcg gcggtcgtca ccgagcttgc cgtccgtctc
     5581 ggccgcggcg acgccccgct cgagcgacgc gcggaggccg accggctccg gtccgacgtg
     5641 gcacgactcc gggccgaggc cgatgctgcg gcgaccgaat cggccgccgc cgagcgggcg
     5701 gccgccacga cgcggactgc cctggaggcc gccacgaccg aggaggctcg cgcggacgcg
     5761 gatcgccgtc gcgccgagga ggccgagcgc ggcgccgccc gcgagctcga ggccgtcgcc
     5821 cgcgaggccg cctggcacgc cgcccaggtc gagcgcctgg ctgccgacgt cgaacgagcc
     5881 cgcgaatccc tcgccgccca tgagtcgacc gggctgtggg gcaacacgac c
//
LOCUS       DATAIW010000198         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183387, whole genome shotgun sequence.
ACCESSION   DATAIW010000198 DATAIW010000000
VERSION     DATAIW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183387"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VHM48_06390"
     CDS             complement(<1..208)
                     /locus_tag="VHM48_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755071.1"
                     /translation="MTVPDDRAVGGLITAGMTVDVLLSAQINIPANVTPPGKYYTDKA
                     TKITYQNMLVLAKTGSGYVVKATLA"
     gene            240..644
                     /locus_tag="VHM48_06395"
     CDS             240..644
                     /locus_tag="VHM48_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755072.1"
                     /translation="MTVSPGSRIENPVVPPGKYYTDKATKITYQNMLVLAKTGSGYVV
                     KATLAQAEEITHLVATGNAQFSMLMRPPSDVRFADASKLGETTNLIIERYGLPVPQNY
                     PVGNGPIQTAAPTATPSLPAAPSGSPGPSAAP"
     gene            complement(826..1470)
                     /gene="coaE"
                     /locus_tag="VHM48_06400"
     CDS             complement(826..1470)
                     /gene="coaE"
                     /locus_tag="VHM48_06400"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2755073.1"
                     /translation="MGDANPTTRAGSVRHTVRIGLTGPIGCGKSTVAGWLADRGAVVI
                     DADLVAREVSAPGQPAHDAILAVFGDGVRVEDRSLDRAALARIVFADPDALRRLEAIV
                     HPLVRARIVAEIETAEAAGAAAVVVEAIKLVEGGLAALCDEVWLVTCEPMVQRDRVIA
                     RGATAEEADRRIAAQASLVERVRPAATRVLDTSGPLVDARSRVAAAWDAALSSR"
     gene            complement(1470..3563)
                     /locus_tag="VHM48_06405"
     CDS             complement(1470..3563)
                     /locus_tag="VHM48_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2755074.1"
                     /translation="MSILRLERVRREVGTFVILDDITAAIAVDDRVGLVGPNGAGKTT
                     LLRLGAGRDEPDEGVVHRKRGLTIGLLAQESHFDEAFMSAPDLRAAVRHGAAHLEAMA
                     ARLAEMEHAGQVVAPGYADLQHQFDILGGYTLDLRVDEALSGLGFTRDEWAKPPTALS
                     GGEQTRATLARLVVAQPDLLLLDEPTNHLDIGAIEWLEEHLRRRHGALLVASHDRAFL
                     DATVNRIWELRDRKLTIFRGDYSAYHRQRGERDVRSVKDSDTQADQIEREKELVQRYR
                     SHRKFSKMHEHEARLEKLQAERVESPKATKKLRLPAGALAGSGPSRSGEIVIRVEGLV
                     VGYLPGRGAVNADGAPATEPKRVATVPFLAATRGDRIGIVGPNGAGKTTLLRTIAGDL
                     PPLDGALSFGNAVQPGYLAQLRDAAIPGATVLDALLEAIPVTAGEARGFLARFLFRGD
                     DVFKEVGKLSGGERSRLELALLGIMPSNLLLLDEPTNHLDIPAREAIEAFMHDSPATL
                     LVVSHDRRLLQTVCTSLWVVDDGLAVPFAGGWSEWRAAVADGWTVHAAAEREAARLRG
                     GAHGGRSAVTSSRTDARATSAGGIAVAERPRRSSVAVRSAAGPAVASTPPSRPAARRE
                     KLSKDAYRRQRTQVDAELTRLGLRKTQLELSMGSPAIAANFVEMRRVASELADVEAAL
                     GAAEDAWLDLEERAP"
BASE COUNT          502 a         1336 c         1319 g          490 t
ORIGIN      
        1 gggcgagggt cgccttgacg acatagcccg acccggtctt ggcgaggacg agcatgttct
       61 ggtaggtgat cttcgtcgcc ttgtcggtgt agtacttgcc cgggggcgtg acgttggcgg
      121 ggatgttgat ctgggccgag aggaggacgt cgaccgtcat cccggcggtg atcaggccgc
      181 cgacggcgcg atcgtcgggg accgtcatcg agaccgccct ccacgccacc gaagtatcgg
      241 tgacggtctc ccccggatcg aggatcgaga acccggtcgt cccgcccggc aagtactaca
      301 ccgacaaggc gacgaagatc acctaccaga acatgctcgt cctcgccaag accgggtcgg
      361 gctatgtcgt caaggcgacc ctcgcccagg ccgaggagat cacccacctc gtcgcgaccg
      421 gcaatgccca gttcagcatg ctgatgcggc cgccgtcgga cgtccgcttc gccgatgcga
      481 gcaagctcgg cgagacgacc aacctgatca tcgagcgcta cggcctgccg gtcccgcaga
      541 actacccggt cggaaacggt ccgatccaga ccgcggcccc gacggcgacc ccgtcgctcc
      601 cggcggcgcc gtcagggagc ccgggcccca gcgcggcgcc ctgacccgag cgtgactccg
      661 cgaacgggac gctgccgggt caagcgcccc cagtcggagt cgatggcgct cacgcggttc
      721 atgccttcgc gccgctgacc cggcgcggcc tcccggccgc ccggcccgcc cccgcgggcc
      781 gggcggccac tccatatctc gctcgcggtc agcttcagcc gacgctcagc ggctgctcag
      841 cgcggcgtcc caggccgcgg ccacccgcga ccgcgcatcg accagcggcc ctgaggtgtc
      901 gagcacccgg gtcgcggccg gtcggacgcg ctcgacgagg ctggcctggg cggcgatccg
      961 gcgatcggcc tcctcagctg tcgctccccg ggcgatcacg cgatcccgct ggaccatcgg
     1021 ctcgcaggtc acgagccaga cctcgtcgca gagcgccgcc agcccgccct cgacgagctt
     1081 gatcgcctcg acgacgaccg ctgccgctcc ggccgcctcg gcggtctcga tctcggccac
     1141 gatccgggcc cggacgaggg ggtggacgat cgcctcgaga cgacgcagcg catcgggatc
     1201 ggcgaagacg atccgggcca gggccgcccg atcgaggctg cggtcctcga cccgtacgcc
     1261 atcgccgaag acggcgagga tcgcgtcgtg agcgggctga ccgggggccg aaacctcgcg
     1321 agcgacaagg tcggcgtcga tcacgacggc gccgcgatcc gccagccagc cggcgaccgt
     1381 actcttgccg cagccgatcg gcccggtcag cccgatgcgg accgtgtgcc ggactgagcc
     1441 ggcgcgggtc gtcgggttcg cgtcgcccat cacggcgccc gctcctcgag atccagccac
     1501 gcgtcctcgg ccgcgccaag cgccgcctcg acgtcggcca gctcgctcgc gacccgtcgc
     1561 atctcgacga agttcgcggc gatcgccggc gagcccatcg acagttcgag ctgggtcttt
     1621 cgcaggccga ggcgggtgag ctcggcgtcg acctgggtgc gctggcggcg gtaggcgtcc
     1681 ttggacagct tctcgcgacg ggcggcaggg cgcgaaggcg gggtgctcgc cacggccggt
     1741 cccgcggccg atcgaacggc gaccgacgac cgacgcggtc gctcggcgac cgcgatcccg
     1801 ccggccgacg tcgctcgcgc atctgtccgg ctgctggtca ccgccgatcg accgccgtgg
     1861 gcaccgccac gaaggcgggc cgcctcgcgt tcggcggccg cgtggaccgt ccagccgtcg
     1921 gccaccgcgg cgcgccattc gctccagccg cccgcgaacg gcaccgcgag cccgtcgtcg
     1981 acgacccaca gggacgtgca gacggtctgg agcaggcgcc ggtcgtgcga gacgacgagc
     2041 agcgtcgccg gcgagtcgtg catgaacgcc tcgatcgcct cgcgggccgg gatgtcgagg
     2101 tggttcgtcg gctcgtcgag gaggagcagg ttcgacggca tgatcccgag cagcgccagc
     2161 tcgagccgcg accgctcgcc gcccgacagc ttgccgacct ccttgaagac gtcatcgccg
     2221 cggaacagga agcgggcgag gaagccacgc gcctcgcccg cggtcaccgg gatcgcctcg
     2281 agcagcgcgt cgaggaccgt ggccccggga atcgccgcgt cgcgcagctg ggcgagatag
     2341 ccgggctgga cggcgttgcc gaagctgagc gcgccgtcga gcggtggcag gtcgcccgcg
     2401 atcgtccgca gcagcgtcgt cttgccggcg ccgttcggcc cgacgatccc gatccggtcg
     2461 ccacgggtcg cggcgaggaa cggcaccgtc gcgacgcgct tcggctcggt cgcgggcgca
     2521 ccgtccgcgt tcacggcgcc acgaccgggc aggtagccga cgacaaggcc ctcgacccgg
     2581 atgacgattt cgcccgatcg cgacgggccg ctgccggcga gggcgcccgc cgggaggcgc
     2641 agcttcttcg tcgccttcgg cgactcgacc cgctcggcct ggagcttctc gagacgagcc
     2701 tcgtgctcgt gcatcttgct gaacttgcgg tgcgatcggt agcgctggac gagctccttc
     2761 tcccgctcga tctggtcggc ctgggtgtcg gagtccttga ccgaccggac gtcgcgctcc
     2821 ccgcgctggc ggtggtaggc gctgtaatcg ccgcggaaga tcgtcagctt ccgatcgcgc
     2881 agctcccaga tccggttgac cgtcgcgtcg aggaacgccc ggtcgtggga cgcgacgagg
     2941 agcgcgccgt ggcgccggcg gaggtgctcc tcgagccact cgatcgcgcc gatgtcgagg
     3001 tggttggtgg gctcgtcgag gagcagcagg tccggctggg cgacgacgag gcgggcgagg
     3061 gtcgcccggg tctgctcgcc acccgacagg gcggtcggcg gcttggccca ctcgtcgcgg
     3121 gtgaagccga gaccggacag ggcttcatcg acgcgcaggt cgagcgtgta gccgccgagg
     3181 atgtcgaact ggtgctggag gtcggcgtag ccgggcgcca cgacctgccc ggcgtgctcc
     3241 atctcggcaa ggcgggccgc catggcctcg agatgcgcgg ccccgtggcg gaccgcggcc
     3301 cgcaggtccg gggcggacat gaacgcctcg tcgaaatggg attcctgggc cagcaacccg
     3361 atcgtcaggc cgcgcttgcg gtggaccacg ccctcgtccg gctcgtcgcg gcccgcgccg
     3421 aggcgcagga gggtcgtctt cccggccccg ttcggcccga ccaggccgac ccggtcgtcg
     3481 accgcgatcg cggccgtgat gtcgtcgagg atgacgaagg tcccgacctc gcgacgcacg
     3541 cgctcgagac gaaggatcga catcggctca gccgcccacg gacgcgcggg cggccgcacg
     3601 ccgggtcgcg gcggcgcgcc gggtccgctc ggcgcgggtc gctcgca
//
LOCUS       DATAIW010000199         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_99647, whole genome shotgun sequence.
ACCESSION   DATAIW010000199 DATAIW010000000
VERSION     DATAIW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99647"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VHM48_06410"
     CDS             <1..1432
                     /locus_tag="VHM48_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX2755075.1"
                     /translation="AQPRQSARGGVYDRFRERVIFPIRDASGGAVGLGGRILPADGGT
                     ESGQGISEVGGGAPRRHPPEDKDHGPKYLNSPATPLFDKSRTLYLIDRAKGPIRKNGQ
                     AVIVEGYTDALMAHQAGFDNVVASLGTALTPGQVALLTRYATKIALAYDVDAAGEKAG
                     TFGAQALESLIGQLAVADTGVELDEVRVVRLPDGKDPDEVVRETPDRWREEVRTAQPI
                     VDYLIDHHARAFDLKTPGGKTRFVKAIVPTLRAIPNPVMRDAYLQTIHRVSGVEERTV
                     LEVLHQRPEAVAVHGRITADAVIGAADALPVDRILQAITPVEEELLRLILLVPDQQLR
                     ASDEIGPDQLPSTPARELFRAIIVQRAPDDHGVNPPFSMSALVAGLDDEIRALAQAIL
                     ARPSPDLAHISAERIAYAVDRCLLRLERIRLDERADWVGAEILAAESRGERESVTRLV
                     DLQRQTNESRRAIDRQIEQATVLVRT"
     gene            1488..3227
                     /gene="rpoD"
                     /locus_tag="VHM48_06415"
     CDS             1488..3227
                     /gene="rpoD"
                     /locus_tag="VHM48_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX2755076.1"
                     /translation="MADPLDKQLVEAVLARPRRAKADVEEARLATRNLRATKVVLPQV
                     ADDEDNDDDDLELGAEPGSDGVVVADGGDVGVEVIGEDGVVVDNKMPSDAPAKLDPKE
                     VDEPTAEELDAISADMIGIDDPVRMYLKEIGKVALLTAEEEVVLAKAIELGEQLVEAP
                     WKGIVSLHEWTRHDTERKTRTTKLQHRLPLGDEAHRLVTDAIHAPEAADLLVAPPDFH
                     LVKAARDAQSDGTKDLLKEAKKLLATYAHKLDADAFLTVLDWSYLAVHNGDLDSRDNV
                     GLRAIYDWTRDDVAFPALERWVTAGNDADLLKKMGFDPEIPLNTKLALRKGELVRIGR
                     DAREQLTSANLRLVVSIAKKYIGRGMSFLDLIQEGNIGLIRAVEKFDFEKGFKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMVETINRLIRVSRQLLQELGREPTVEEIAEAMSK
                     GQEVQVTPEKVREIIKVSQEPVSLETPIGEEEDSHLGDFIEDRGALAPAEAASHQLLK
                     EQVEAVLDSLTGRERRVLQLRFGLEDGRARTLEEVGREFNVTRERIRQIEAKALRKLR
                     HPSRSRKLKDYLE"
BASE COUNT          552 a         1184 c         1136 g          441 t
ORIGIN      
        1 cgctcagccg cgccagtcgg cccggggcgg cgtgtacgac cgcttccgcg agcgggtcat
       61 cttcccgatc cgcgacgcca gcggcggcgc ggtcggtctc ggcgggcgga ttcttcccgc
      121 ggacggcggg acggagagcg ggcagggcat ctccgaggtc ggaggcggag ctccgcgacg
      181 ccatccgccc gaggataagg accatgggcc caagtacctc aactcgcccg cgacgccgct
      241 cttcgacaag tcccggacgc tctacctgat cgaccgggcc aagggtccga tccggaagaa
      301 cggccaggcg gtgatcgtcg agggctacac cgacgccctg atggcccacc aggcgggttt
      361 cgacaacgtc gtcgcgtcgc tcgggacggc cctgacgccg ggccaggtcg cgctcctcac
      421 ccgctacgcg acgaagatcg ccctcgccta cgacgtcgac gcggccggcg agaaggcggg
      481 gacgttcggc gcccaggcgc tcgagtcgct gatcggccag ctcgcggtcg ccgacaccgg
      541 cgtcgagctc gacgaggtcc gcgtcgtccg cctgcccgac ggcaaggatc ccgacgaggt
      601 cgtccgcgag acgcccgatc gctggcgcga ggaggtccgg accgcccagc cgatcgtcga
      661 ctatctcatc gaccatcacg cccgcgcgtt cgatctcaag acccccggcg gcaagactcg
      721 cttcgtcaag gcgatcgtgc cgactctccg ggcgatcccc aacccggtca tgcgcgacgc
      781 ctatctccag acgatccatc gcgtgtcggg cgtcgaggag cggacggtcc tcgaggtcct
      841 ccatcagcga cccgaagccg tggccgtcca cggccggatc accgccgacg ccgtgatcgg
      901 cgcggccgat gccctcccgg tcgaccggat cctccaggcg atcaccccgg tcgaggagga
      961 gctcctccgg ctgatcctgc tcgtccccga ccagcagctc cgggcgtccg acgagatcgg
     1021 gcccgaccag ctgccctcga ccccggcccg cgagctgttc cgggcgatca tcgtccagcg
     1081 cgcgcccgac gaccatggcg tcaacccgcc gttctcgatg tcggccctgg tggccggtct
     1141 cgacgacgag atccgggccc tcgcccaggc gatcctggcc cggccgtcgc cggacctggc
     1201 ccacatcagc gccgagcgga tcgcctacgc cgtcgaccgc tgcctgctcc ggctcgagcg
     1261 gatccggctc gacgaacggg ccgattgggt cggcgccgag atcctcgcgg ccgagagccg
     1321 cggcgagcgc gagtccgtga cccgcctcgt cgacctccag cgccagacca acgaatcgcg
     1381 gcgggccatc gatcgccaga tcgagcaggc caccgtgctg gtccgaacct gacccatgcc
     1441 cctgactgaa ctctcgacct cgcaacccga tacccggagg cacactcatg gctgaccccc
     1501 tcgacaagca gctcgtcgaa gcggttcttg ctcgtccgcg gcgggccaaa gccgacgtcg
     1561 aggaggcccg cctggcgacc cgcaacctgc gggcgaccaa ggtcgtcttg ccgcaggttg
     1621 cggacgacga ggacaacgac gacgacgatc tcgagctggg cgccgagccc gggtccgacg
     1681 gggtcgtcgt ggccgatggc ggcgatgtcg gcgtcgaggt gatcggcgag gacggcgtcg
     1741 tcgtcgacaa caagatgccg agcgacgccc cggccaagct cgacccgaag gaggtcgacg
     1801 agccgaccgc ggaggagctc gacgcgatca gcgccgacat gatcgggatc gacgacccgg
     1861 tccggatgta cctcaaggag atcggcaagg tcgccctcct cacggccgag gaggaggtcg
     1921 tcctggccaa ggcgatcgag ctcggcgagc agctggtcga ggcgccgtgg aaggggatcg
     1981 tctcgctcca cgaatggacc cgccacgaca ccgagcgcaa gacccggacg acgaagctgc
     2041 agcatcggct gcccctgggc gacgaggccc atcggctcgt gacggacgca atccacgccc
     2101 cggaagcggc cgacctgctc gtcgcgccgc ccgacttcca cctcgtgaag gccgcccggg
     2161 acgctcagtc ggacgggacg aaggacctcc tgaaggaagc caagaagctg ctcgcgacct
     2221 acgcccacaa gctcgacgcg gacgccttcc tgacggtcct cgactggtcc tacctcgcgg
     2281 tccacaacgg cgatctcgac tcgcgcgaca acgttggcct gcgggcgatc tacgactgga
     2341 cccgcgacga cgtggcgttc ccggccctcg agcgctgggt cacggccggc aacgacgccg
     2401 atctgctcaa gaagatgggc ttcgaccccg agattccgct caacaccaag ctggccctcc
     2461 gcaagggcga gctcgtccgg atcggacgtg atgcgcgtga gcagctgacc tcggccaacc
     2521 tgcggctggt ggtctcgatc gcgaagaagt acatcggccg ggggatgtcg ttcctcgacc
     2581 tgatccagga aggcaacatc gggctgatcc gggcggtcga gaagttcgac ttcgagaagg
     2641 gtttcaagtt ctcgacgtat gccacgtggt ggattcgcca ggcgatcacc cgggcgatcg
     2701 ccgaccaggc ccggacgatc cggatcccgg tccacatggt cgagacgatc aaccgcctga
     2761 tccgcgtctc gcgccagctg ttgcaggagc tggggcgcga gccaacggtc gaggagatcg
     2821 ccgaggcgat gtcgaagggc caggaggtcc aggtcacccc cgagaaggtc cgcgagatca
     2881 tcaaggtgtc gcaggagccg gtctcgctcg agaccccgat cggcgaggag gaggactctc
     2941 acctcggcga cttcatcgag gaccgggggg cgttggcgcc cgctgaggcg gcgtcgcacc
     3001 agctgctcaa ggagcaggtc gaggcggtgc tggattcgct gaccggtcgc gagcggcgcg
     3061 tccttcagct ccggttcggg ctcgaggacg gccgggcgcg gaccctcgag gaggtcggcc
     3121 gcgagttcaa cgtgacccgc gagcggatcc gccagatcga ggcgaaggcc ctccgcaagc
     3181 tccgccaccc gagccggagc cgcaagctca aggactacct cgagtaggtt cggagttccg
     3241 gtctcgcggc ggcggcccag gcgcagggcg gcagcccggg cgcaggacgg aggcccgggc
     3301 gcaggacggg cgc
//
LOCUS       DATAIW010000200         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_10097, whole genome shotgun sequence.
ACCESSION   DATAIW010000200 DATAIW010000000
VERSION     DATAIW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10097"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VHM48_06420"
     CDS             <1..682
                     /locus_tag="VHM48_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2755077.1"
                     /translation="VGALCRQRERGSDARPDDQRIRHPNRHAGAQPDADPDADRSPTI
                     GEPADDGARIIAVQTNPLYGPVPVTRARDITIDSPAVGIVQVQLLLPSRFDAQPEKRW
                     PALYVLGGSSGTHRSWTQQTDIEALTAPEDLLVVIPDQGAEIGGGGYADWWNGGKGGP
                     QMWETFHVVELPQLLERNWHVGDGRAIEGPSSGGLGAIGYAERHPGMFLFVGSWSAPG
                     PEWPNLLP"
     gene            679..1026
                     /locus_tag="VHM48_06425"
     CDS             679..1026
                     /locus_tag="VHM48_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755078.1"
                     /translation="MSPGEGHFNLLGDPVEQADLCRAHDPMINAAALRGTTLYIAYGN
                     GELRPFDIGPDNPTGPGEREYGIESAAFVKRLHELKIPVTVYAYGPGTQTTPYMVRDL
                     HVSFPLVLKVLGL"
     gene            1152..>3125
                     /gene="dnaE"
                     /locus_tag="VHM48_06430"
     CDS             1152..>3125
                     /gene="dnaE"
                     /locus_tag="VHM48_06430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2755079.1"
                     /translation="MTTIAPNDFTHLHVHSEFSLLDGLGRITDLVDSAAAQGFDSLAI
                     TDNGALYGAVAFYQAAKAKGIKPIIGVETYVARRSMTEKEGKADAQPFHLILLAKDLV
                     GYRNLCRLITDAHVDGYYYKPRIDHEHLAKYSEGLVGLSACLGGEIPRALEIEDWASA
                     RSLAAEYQDILGKGNFFLELQDHGMPEQRVLNEKLLRLAPETGVPLVVTNDLHYVHEA
                     QSAAQDVLLCIGTGNNIDTPGRMKFATNDFWLKSAAQMAALFPDQREAILNTRRIAEM
                     TEIELPLGQLRIPHFPVPEGHTVETWLRAECQRGLAERYGTVTPELQKRLDYELGVIL
                     SMGYAGYFLIVADFVRFAREQRIQTTCRGSAPGSIVTYTLYITPVDPIQYGLPFERFL
                     NPDRVTMPDIDVDFEDERRDEVINYVSRKYGSDHVAQIITFGTMLARAAIRDVGRVLG
                     HSYGEVDRIAKAVPNQLGIKLDEALTMSPDLKGQVDADPAVKRIIDFARQLEGVARNA
                     STHAAGVVISREPLTELMPLQKATNSDALMSQYEMHAIEALGLLKFDFLGLSNLTILR
                     HAVDLVRHERGIEIDLDHIPLDDATTFALLASGETTGIFQLESAGMRRYIRDLQPTSV
                     LDLAAMVALYRPGPMDNIPAYIRRKHGQEKVTYL"
BASE COUNT          531 a         1154 c          987 g          453 t
ORIGIN      
        1 tgtcggcgcg ctgtgccggc aacgcgaacg tggctccgac gcccggcccg acgaccagcg
       61 catccgacac cccaaccgcc acgcgggcgc ccagccggac gccgatcccg acgccgaccg
      121 ctctccgacc atcggcgagc cggccgacga tggcgcgcgc atcatcgcgg tccaaacgaa
      181 cccgctctac ggtcccgtcc cggttacccg ggcaagagac atcacgatcg attcgccggc
      241 cgtcggcatc gtgcaggtcc agctgctcct cccgagccgg ttcgatgccc agccggagaa
      301 acgctggccg gcgctgtacg tgctcggcgg ctcctccggg acacaccggt cctggacgca
      361 gcagacggac atcgaggcgc tgaccgcccc agaggacctg ctggtcgtca ttcccgatca
      421 aggtgccgag atcggtggtg gcgggtacgc ggactggtgg aatggcggca agggcggccc
      481 gcagatgtgg gagaccttcc acgtggtcga gctgccccag ttgctcgagc ggaactggca
      541 tgtcggcgac ggacgtgcga tcgagggccc gtcgtcgggc ggtctcgggg cgatcgggta
      601 tgccgagcgt catccgggga tgttcctgtt cgtcgggtca tggagcgcac ctggacccga
      661 atggcccaac ctccttccat gagccctggg gaaggccatt tcaacctctt gggtgacccc
      721 gtcgagcagg ccgacctgtg cagggcgcac gacccgatga tcaacgccgc ggccctccgg
      781 ggcaccacgc tctacatcgc ctacggcaac ggcgagctga gaccgttcga catcggtccg
      841 gacaacccga ccggtcccgg cgagcgggag tacgggatcg agagcgcggc cttcgtcaag
      901 cggctccacg agctgaagat cccggtgacc gtctacgcct acgggccggg cacacagacc
      961 acgccctaca tggtgcgcga ccttcatgtc tcattcccgc tcgtcctcaa ggtgctcggc
     1021 ttgtagggct gccggccgcg gacggcgatc caacagcggg agctcgcggt gacgcgcgcg
     1081 tgtcgaccgc tcgtcgcggc cgcctcgtaa cgcccctccc gccccccgag ccgggctacc
     1141 attggcctcc cgtgacgacc atcgcgccga acgatttcac gcacctccac gtccactccg
     1201 agttcagcct cctcgacggg ctcgggcgga tcacggacct cgtcgattcg gcggccgcgc
     1261 aggggttcga cagcctcgcg atcaccgaca acggcgccct ttacggcgcc gtcgccttct
     1321 accaggcggc gaaggcgaag gggatcaagc cgatcatcgg cgtcgagacc tacgttgccc
     1381 gccggtcgat gaccgagaag gagggcaagg ccgacgccca gcccttccac ctgatcctcc
     1441 tggccaagga tctcgtcggc taccgcaacc tctgccggct catcaccgac gcccacgtcg
     1501 acggttacta ctacaagccc cggatcgacc acgagcacct cgcgaagtac agcgagggcc
     1561 tcgtcggcct gtcggcctgc ctcggcgggg agatcccgcg ggccctcgag atcgaggact
     1621 gggcgagcgc ccggagcctc gccgccgagt accaggacat cctcggcaag ggcaacttct
     1681 tcctcgagct ccaggaccac gggatgccgg agcagcgcgt cctcaacgag aagctgcttc
     1741 gcctggcacc cgagaccggc gtgccgctcg tggtgaccaa cgacctgcac tacgtccacg
     1801 aggcgcagag cgccgcccag gacgtcctcc tttgcatcgg gaccggcaac aacatcgaca
     1861 cgccgggccg gatgaagttc gcgacgaacg acttctggct caagtcggcc gcccagatgg
     1921 cggcgttgtt ccccgaccag cgcgaggcga tcctcaacac ccgccggatc gccgagatga
     1981 ccgagatcga gctgccactc ggccagctcc ggatcccgca tttcccggtc cccgagggcc
     2041 acacggtcga gacatggctt cgggccgagt gccagcgcgg cctcgccgag cgctacggga
     2101 cggtgacgcc ggagctccag aagcggctcg actacgagct cggcgtgatc ctctcgatgg
     2161 gctacgccgg ctacttcctg atcgtcgccg acttcgtccg gttcgcccgg gagcagcgga
     2221 tccagaccac ctgccggggc tcggcgccgg gctcgatcgt gacctacacg ctgtacatca
     2281 cgccggtcga cccgattcag tacggcctgc cattcgagcg cttcctcaac cccgaccggg
     2341 tgacgatgcc ggacatcgac gtcgacttcg aggacgagcg ccgcgacgag gtcatcaact
     2401 acgtgagccg caagtacggc tcggaccacg tcgcccagat catcaccttc ggcacgatgc
     2461 tcgcccgggc cgcgatccgc gacgtcggcc gggtcctcgg ccacagctac ggcgaggtcg
     2521 accggatcgc gaaggccgtg ccgaaccagc tcggcatcaa gctcgacgag gcactgacga
     2581 tgtcacccga cctgaaaggg caggtggacg ccgacccggc cgtcaagcgg atcatcgact
     2641 tcgcccgcca gctcgagggc gtcgcccgca acgcctcgac ccacgccgcc ggggtcgtga
     2701 tcagccgcga gccgctgacc gagctgatgc cgctccagaa ggcgacgaac tccgacgccc
     2761 tgatgagcca gtacgagatg cacgcgatcg aggcgctcgg cctgctcaag ttcgacttcc
     2821 tcggcctctc gaacctgacg atcctgcgcc acgccgtcga cctggtccgg cacgagcgcg
     2881 gcatcgagat cgacctcgat cacatcccgc tcgacgacgc gacgacgttc gcgctcctcg
     2941 cctcgggtga gacgaccggc atcttccagc tcgagtcggc cgggatgcgc cgctacatcc
     3001 gcgacctcca gccgacctcg gtcctcgacc tcgcggcgat ggtcgcgctc taccggccgg
     3061 gcccgatgga caacatcccg gcctacatcc ggcgcaagca cggccaggag aaggtcacct
     3121 acctc
//
LOCUS       DATAIW010000201         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_272737, whole genome shotgun sequence.
ACCESSION   DATAIW010000201 DATAIW010000000
VERSION     DATAIW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272737"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VHM48_06435"
     CDS             <1..810
                     /locus_tag="VHM48_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX2755080.1"
                     /translation="KEYFGSWRTFPDTCDWSWPETKPVGFHQTYLGIDVFEGAYQYDG
                     MRIVPGWGGSMFEALMPAMFVPEDQWGKGSWAINHPLTVAAQIHHGLVEAGYGYWGFS
                     PSDKPEGGYAAYGVDAIGMNPDGYPSNEDNTFVDHGFAGCPGAHEAKPDPAPSAYTNG
                     VVTPHASFLALRYAPKASLANLAKLEHDFKIYDKWGFRDSVNVGTGAISNGYLSLDQG
                     IIMAAIGNALGHDMLLHAFATPQFERVLEPIVSMERFNAAPRDRVARLDQP"
     gene            822..>1616
                     /locus_tag="VHM48_06440"
     CDS             822..>1616
                     /locus_tag="VHM48_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755081.1"
                     /translation="MTTRRPPSRSPGGTQPVAHAADLPAATTAGAARIAWFATRGALT
                     DRDRPAPPTTLPPPDDVRAVRGRGQVTIDWSPVVGAAGYLVHRGQTADGPFEPIDLDV
                     GDILAVPHGPYLDTTGTPGTAAWYAVSSLPTIETEGGALSPPVAARAGSDNGAVAVRV
                     EAGRVVGRVARPLRPMIGSEHLALLLRGAGPGGSDVGAELAEAFRIVGRELGVEAVRA
                     HAILDDSLGVYRDAGEGPIHDFSTVDEVLDRLLDTGLRPIVELSFMP"
BASE COUNT          246 a          587 c          547 g          236 t
ORIGIN      
        1 aaggagtatt tcgggtcctg gcgcacgttc cccgatacct gcgactggag ctggccggag
       61 accaaaccgg tcggcttcca ccagacgtac ctcggcatcg acgtcttcga aggtgcctac
      121 cagtacgacg gcatgcgcat cgtcccgggc tggggcggca gcatgttcga ggcgctcatg
      181 ccggccatgt tcgtccctga ggaccagtgg ggcaagggca gctgggcgat caatcatccc
      241 ctgaccgtgg ccgcccagat ccatcacggg ctcgtcgagg ccggctatgg ctactgggga
      301 ttctccccgt ccgacaagcc cgagggcggc tacgcggcgt atggggtcga cgcgatcggg
      361 atgaacccgg acggctatcc atcgaacgag gacaacacgt tcgtcgacca cgggttcgcc
      421 ggctgcccgg gcgcgcatga agcgaagccc gacccggcgc cgtcggcgta cacgaacggc
      481 gtcgtgacgc cgcacgcatc gttcctcgcc ttgcgctacg cgcccaaggc gtcgctcgcg
      541 aacctggcca agctcgaaca cgacttcaag atctacgaca agtggggatt ccgcgactcg
      601 gtgaacgtcg ggacgggcgc gatctcgaac ggctacctgt cgctcgacca gggcatcatc
      661 atggccgcca tcgggaacgc gctcggccat gacatgctcc tgcacgcgtt cgcgaccccg
      721 cagttcgagc gggtgctgga gccgatcgtc tcgatggaac ggttcaacgc cgccccacgc
      781 gaccgtgtcg ctcggctcga ccagccgtag acctcgagcc gatgacgaca cgccgcccgc
      841 ccagccggtc gcccggcggg acccagccgg ttgcgcacgc cgccgacctg ccagccgcga
      901 cgaccgccgg cgccgcgcga atcgcctggt tcgcgacacg tggcgccctg acggaccgcg
      961 accggccggc gccgccgacc acgctcccgc cgccggatga cgtccgggcg gtacgtggcc
     1021 gcgggcaggt aacgatcgat tggtcgccgg tcgtgggtgc cgccggctac ctcgtccacc
     1081 ggggccagac cgccgacggt ccgttcgagc cgatcgacct ggacgtcggc gacatcctcg
     1141 cggtgccgca cggcccctac cttgacacga cgggcacccc tggcacggcg gcgtggtacg
     1201 cggtctcgag cctgccgacc atcgagacgg agggaggggc gttgtcgccg ccggtcgcgg
     1261 cccgagccgg gagcgacaac ggcgccgtcg cggtccgcgt cgaagccgga cgcgtcgtcg
     1321 gtcgtgtcgc gcgaccattg cgtccgatga tcggctccga acacctcgcc ctcctcctcc
     1381 gcggggccgg gcccggcgga tcagacgtcg gcgcggaact ggccgaggcc ttccggatcg
     1441 tgggccgcga actcggcgtc gaggccgtgc gggcccatgc catcctcgat gactcactcg
     1501 gcgtctaccg cgacgcgggc gaagggccca tccacgactt ctcgacggtc gacgaggtcc
     1561 tggaccggct actcgatacc ggtctgcggc cgatcgtcga gctctcgttc atgccg
//
LOCUS       DATAIW010000202         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_99756, whole genome shotgun sequence.
ACCESSION   DATAIW010000202 DATAIW010000000
VERSION     DATAIW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99756"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VHM48_06445"
     CDS             <1..1434
                     /locus_tag="VHM48_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755082.1"
                     /translation="AGVFVDRLDRRLVLILTNVLRGLAFIGMFFVGTNLVLIYLLNII
                     VSTITVFFAPAEAAMIPNLVPRKQLLSANGIFTLTLNAAFALGFTLVGPLIVKIFGAS
                     ALIIVVAALYFVAAGFCSTLPSAPPPSSNAGPKGARGRVREAENAVGSVLLQLREGLA
                     YIRGHPEIRWSLTYLGIAASLVGVLGVLGPNFAQKTLGLGPEDFVVVVLPLGVGIVMG
                     ILLLNAYGRLLPRRRVIEGGLIALGIFIAAMAGSGTISKFLGSSAARTGVDLSLVTSL
                     LSIVVAVAFFAGISYAAVAIPAQTQLQEDLPEDVRGRVFGVLNMLVSVASFLPILIVG
                     PIADLLQALTGQGTTIVLIIVAILIAASGIASIRLRGPLRAVERDMRATVGTDRDPFV
                     TALGVEARAGAIDLGGEPIADPGPLAGDQADAGPIVLPAADPITAAQGALDLDVDTRA
                     IEIGDQPGEDDQMAEDAAGIVDRSRPA"
     gene            1443..2465
                     /locus_tag="VHM48_06450"
     CDS             1443..2465
                     /locus_tag="VHM48_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX2755083.1"
                     /translation="MPRVAVVFTGGTISMRHDPVAGGNVPVLGGAALLETAPGLDAIA
                     DLVPIDHGLTPASHFTFEALFGISVPVRDALADPTIDGAVVVQGTDTIEETAFFLDLL
                     HRGPKPIVVTGAMRSASEPGYDGPANLTAAVRVASSVEVGRADVGAVVVLGGTIEAAD
                     DVTKTHASAFDTFRGLNRGSLGRVEDDRIVLTSERGPRRQVATERAAARVFLVTATVA
                     TDGTPIAALHAAGADGFVVAATGAGNTAPALLAAAEAAIADGLPVVLTTRCPIGAAGA
                     GYAFPGGGATWLRAGAILAGHLGGPKARIALALGLGAGLGRDGLAALLADPVPIHDPE
                     RYWVDR"
     gene            2465..4114
                     /locus_tag="VHM48_06455"
     CDS             2465..4114
                     /locus_tag="VHM48_06455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2755084.1"
                     /translation="MPLDVLVTGGRIATLAGDRGFGWVEAVGITDGRVAFAGSAIDLE
                     TRADPHTLRVEMDPDEVAIPGLTDAHLHLAEGGLSLDRVDLTATATLDDGLAAIAAAH
                     VALVDRDAWLEGHGWHNDRWGVWPTADDLERVAPGRAVALWAHDHHALWVSRRALEIG
                     GIDAARTDPDGGIIRRDADGAPTGVLHESAARIVMDRVPPASAEQYEASIARLARDLV
                     GLGVVAVHDPGALSIQEGLGRGIAAYRILDERGDLPIRVHASIRSEQIVTAAGLELRS
                     GDPLGPPGGRARFGWLKLFADGTLASRTAALLGPIEPEVGRPLPPGTERGIWLTDPEE
                     VAAFARQAADAGIATQIHAIGDRSCRVSLDALEPTAGRTALMPRLEHVQMLHPDDRPR
                     FARAGIAASIQPVHVRADAPSARLLWGDRAETRGYPMRSLLEAGAVVAFGTDAPVEPI
                     DPWPGLSMAVTRIDGTWPAGAEPFGPDERLTLAEALRAACVAPAVTAAEPDRGRLVPG
                     QRADLAILPASALSHPIEPGGALATARPRLVMIDGEVAFER"
     gene            4211..>5061
                     /locus_tag="VHM48_06460"
     CDS             4211..>5061
                     /locus_tag="VHM48_06460"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX2755085.1"
                     /translation="MHCHANARLTPRGRGEVFAAVEAGMTVSAACLAFRLSRRTYYRW
                     LPRWRADGAAGLLDRSSRPRRSPQRLSVAAERQIAGLREATGFGPDRIGALLGLPAST
                     CHRALGRLGYLERVRVSEPVVRYEIAEPGGLLHLDTKKLGRLGAGPGHRATGDRTRRN
                     RGIGWEVLHVAIDAATRLVYAELLADEKGRTTARFLVRAVRWFRARGIRVVRLLTDNG
                     SPYRSRVFGRARRWLGLRHSRTRPYRPQTNGKAERWIRTVLSECLYVEVFHSPGERRL
                     ALERFID"
BASE COUNT          627 a         1858 c         1806 g          770 t
ORIGIN      
        1 gccggggtct tcgtcgaccg cctcgaccgg cgcctggtcc tgatcctcac gaacgtcctg
       61 cgcgggctgg cgttcatcgg catgttcttc gtcgggacga acctcgtcct gatctacctc
      121 ctgaacatca tcgtctcgac gatcaccgtc ttcttcgccc cggccgaggc ggcgatgatc
      181 ccgaatctgg tgccgcgcaa gcagctcctc tcggcgaacg ggatcttcac gctcacgctg
      241 aacgcggcgt tcgccctcgg gttcacgctg gtcggcccgc tgatcgtcaa gatcttcggc
      301 gcgtccgcgc tcatcatcgt cgtcgcagcc ctctatttcg tcgccgccgg cttctgctcg
      361 acgctgccgt cggcgccgcc gccctccagc aacgccggcc cgaagggcgc ccgcggtcgc
      421 gtccgcgaag ccgagaacgc tgtcggatcg gtgctcctcc agctgcgcga gggcctcgcc
      481 tacatccgcg gccatccgga gatccgctgg tcgctgacct acctcggcat cgccgcctcg
      541 ctggtcggcg tcctcggcgt cctcgggccg aacttcgcgc agaagaccct gggcctcggg
      601 ccggaggact tcgtcgtggt cgtcctgccg ctcggggtgg ggatcgtgat gggaatcctg
      661 ctgctcaacg cctacggccg cctgcttccc cgccggcgcg tgatcgaggg tggattgatc
      721 gccctcggca tcttcatcgc cgccatggcg ggctccggga cgatcagcaa gttcctcggt
      781 agctcggcgg cccggaccgg cgtcgacctg tcgctcgtga catcgctcct ctcgatcgtg
      841 gttgctgtcg ccttctttgc cgggatctcc tacgccgcgg tcgccatccc cgcccagacc
      901 cagctccagg aggatctccc ggaggatgtc cgggggcgcg tcttcggcgt cctgaacatg
      961 ctcgtgtcgg ttgcgagctt cctgccgatc ctcatcgtcg gcccgatcgc cgacctcctc
     1021 caggccctga cgggtcaggg gaccacgatc gtcctgatca tcgtcgcgat cctgatcgcg
     1081 gcctcgggga tcgcctcgat ccgtctccgg gggccgttgc gggccgtcga gcgcgacatg
     1141 cgggccacgg tcggcaccga ccgcgacccg ttcgtgaccg cgctcggggt cgaagcgcga
     1201 gcgggcgcca tcgacctcgg cggcgaaccg atcgcggatc ccggcccgct ggcgggcgac
     1261 caagcggacg ccggtccgat cgtcctgccc gcggcggacc cgatcaccgc ggcccagggc
     1321 gcgctcgatc tcgacgtcga cacccgtgcg atcgagatcg gcgaccagcc tggcgaggac
     1381 gatcagatgg cggaggatgc ggccgggatc gtcgaccgca gccggccggc ctgagtcgac
     1441 cgatgccgcg cgtcgccgtc gtcttcaccg gggggacgat cagcatgcgc cacgatccgg
     1501 tcgcaggtgg caatgtcccg gtcctcggcg gcgcggccct gctcgagacc gcacccggcc
     1561 tcgacgcgat cgccgacctc gtcccgatcg accacggcct gaccccggcg agccatttca
     1621 cgttcgaggc gctgttcggg atctcggtgc cggtccggga tgccctcgcg gatcccacca
     1681 tcgacggcgc ggtcgtcgtc cagggaacgg acacgatcga ggagacagcg ttcttcctcg
     1741 atctccttca tcggggcccg aagccgatcg tcgtgaccgg cgcgatgcgg tccgccagcg
     1801 aacccggcta cgacggcccg gcgaacctga ccgcggcggt ccgggtggcg tcgtccgtcg
     1861 aggtcgggcg ggcagatgtc ggtgcggtcg tcgtcctcgg cggaacgatc gaggccgccg
     1921 acgacgtgac caagacgcat gcctcggcgt tcgacacgtt ccgcggcctc aaccgcggat
     1981 cgctcggtcg ggtcgaggat gaccggatcg tgctgacctc ggagcgggga ccacggcggc
     2041 aggtcgcgac ggaacgggcg gcggcgcggg tcttcctcgt gaccgcgacc gtggcaacgg
     2101 acggaacgcc gatcgcggcc ctccacgcgg cgggcgccga cggcttcgtc gtggccgcga
     2161 ccggcgccgg gaataccgca ccggccctgc tcgcggcggc ggaagccgcg atcgccgatg
     2221 ggttgcccgt cgtgctgacc acgcgctgcc cgatcggggc ggcaggggcg ggctatgcct
     2281 tccccggtgg cggtgccacc tggttgcgcg caggcgcgat cctggcgggt catctcggcg
     2341 gcccgaaggc ccggatcgcg ctggcgctcg gcctgggcgc ggggctcggt cgcgacggcc
     2401 tcgcggccct gctcgctgat ccggtcccga tccacgatcc ggagcgctac tgggtggacc
     2461 gctgatgccc ctcgacgtcc tcgtgaccgg tggccggatc gcgaccctgg ccggcgatcg
     2521 cggcttcgga tgggtcgaag cggtcggcat caccgatggc cgggtggcgt tcgccggatc
     2581 ggcgatcgat ctcgagacgc gggcggatcc ccacaccctc cgggtcgaga tggatcccga
     2641 cgaggtcgcg atcccgggcc tgaccgacgc ccacctccat ctcgccgagg gcggcctctc
     2701 gctcgaccgg gtcgacctga ccgcaaccgc gacgctcgac gacggcctgg ccgcgatcgc
     2761 ggcggcccat gtggcgcttg tggatcggga cgcctggctc gaaggccatg gctggcacaa
     2821 tgaccgctgg ggcgtctggc caacggccga cgatctcgag cgggtcgcgc cgggccgggc
     2881 ggtcgcgctg tgggcccacg accaccacgc gctctgggtg agccggcggg cgctcgagat
     2941 cgggggcatc gatgcggctc gcacggaccc cgacggcggg atcatccggc gcgatgcgga
     3001 cggcgcgccg accggtgtcc tccacgagtc tgccgccagg atcgtgatgg accgggtgcc
     3061 gcctgcctcg gccgagcagt acgaggcatc gatcgcccgg ctcgcccgcg acctcgtggg
     3121 actcggggtc gtcgcggtcc acgacccggg ggcgctgtcc atccaggagg gcctggggcg
     3181 ggggatcgcc gcataccgga tcctcgacga gcgcggcgac ctgccgatcc gggtccatgc
     3241 atcgatccgc tccgaacaga tcgtgacggc agcagggctg gagctgcgga gcggcgaccc
     3301 gttgggcccg cccggtggcc gcgcccggtt cggctggctg aagctgttcg ccgacggcac
     3361 gctcgcgtcg cggacggcgg cgctcctcgg gccgatcgag ccggaggtgg gccggcccct
     3421 gccgcccggc accgagcgcg ggatctggct gactgacccc gaggaggtcg cggccttcgc
     3481 ccggcaggcg gccgatgccg ggatcgcgac ccagatccac gcgatcggcg accggagctg
     3541 tcgggtgtcg ctcgacgcgc tcgagccgac cgccggccgg accgccctga tgccgcggct
     3601 cgagcatgtc cagatgctcc acccggacga tcgacctcga tttgcccggg ccgggatcgc
     3661 cgcctcgatc cagccggtcc acgtccgggc cgacgcgccc agcgcacggc tcctgtgggg
     3721 cgatcgagcg gagacgcgcg gctatccgat gcggtcgctg ctcgaggccg gtgccgtcgt
     3781 ggcgttcggg accgacgccc cggtcgagcc gatcgacccg tggcccgggc tgtcgatggc
     3841 cgtgacgcgg atcgacggga cgtggccggc cggcgccgaa ccgttcggtc cggacgagcg
     3901 gctgaccctc gcggaggcgc tccgggcggc ctgtgtcgcg ccggccgtca ccgccgccga
     3961 gccggatcgc gggcgcctgg tccccggtca gcgggcggat ctcgcgatcc tgccggcgag
     4021 cgccctgtcc catccgatcg agcccggtgg cgcgctggcc acagcccgtc cccgcctcgt
     4081 gatgatcgac ggcgaagtcg cgttcgagcg ctaggtctgt tctcccacga ggttggtgac
     4141 acacgaggag cccgtcacca gttaggttga aggcgctgac ctatcaactg acctgggagg
     4201 gctcctcggg atgcattgtc acgccaacgc ccgtctgaca ccacggggcc ggggcgaggt
     4261 cttcgcggcc gtcgaggcgg ggatgaccgt gagcgccgcc tgtctcgcct tccggctgag
     4321 ccgccggacc tactaccgct ggctgccgcg ctggcgggcc gacggcgcgg cgggcctcct
     4381 cgaccggagc tcgcgaccac ggcgcagccc gcagcgcctg tcggtcgcgg ctgaacgcca
     4441 gatcgccgga ctacgcgagg cgaccggctt cggtcccgac cggatcggtg ccctgctcgg
     4501 tctgccggcc tcgacctgcc atcgagccct cggtcggctc ggctatctcg agcgggtccg
     4561 ggtcagcgag ccggtcgtcc gctacgagat cgccgagccc ggcggcctgc tccacctcga
     4621 caccaagaag ctcggccggc tgggcgcggg tcccggccac cgcgcgaccg gcgatcggac
     4681 gaggcgcaac cgggggatcg gctgggaggt cctccatgtc gcgatcgatg ccgccacccg
     4741 gctcgtctac gccgagctgc tggccgacga gaagggccgg acgacggccc gcttcctggt
     4801 ccgggcggtg cgctggttcc gggcgcgcgg gatccgggtc gtccggctcc tgaccgacaa
     4861 cggatcgccc tatcgcagcc gggtgttcgg tcgagcccgg cgctggctcg gcctgcgcca
     4921 cagccggacc cggccgtatc ggccccagac caatggcaag gccgagcgct ggatccggac
     4981 ggtcctgtcc gaatgcctct acgtcgaggt cttccactcg cctggcgagc gtcgacttgc
     5041 cctcgagcgg ttcatcgact a
//
LOCUS       DATAIW010000203         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_10223, whole genome shotgun sequence.
ACCESSION   DATAIW010000203 DATAIW010000000
VERSION     DATAIW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10223"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..356)
                     /locus_tag="VHM48_06465"
     CDS             complement(87..356)
                     /locus_tag="VHM48_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755086.1"
                     /translation="MIVARGFGRFAEPARLVLAVLRDGPRTAVHLFDAVRALDGPVGP
                     GTLYAAIARLERRGLIEGRAGSDGRRSYLLTDLAIRIGAVPGGAG"
     gene            complement(358..921)
                     /locus_tag="VHM48_06470"
     CDS             complement(358..921)
                     /locus_tag="VHM48_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX2755087.1"
                     /translation="MRVLRRLILSIGMLGVALAVVGAFAAQSLAGGATQAGATIVDAS
                     GATVGWARLVEDASGIVHVNVHVAGLTPGLHGIHIHSIAACTPTFAAAGGHYNPLVRQ
                     HGLLNTSGAHAGDLPNLIVNGDGIGHLDATTDRVTLKNGPTTLFDTTSGAVGSSFIIH
                     ANEDDQLTDATNGGSGGRIACGVIVAR"
     gene            1032..1826
                     /locus_tag="VHM48_06475"
     CDS             1032..1826
                     /locus_tag="VHM48_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755088.1"
                     /translation="MTSTRLWDRAADLPMVAGRRPRPEIVALPAAGLPTVAELFTFMR
                     DAELRFDALRMRIEERSFGVAGEQVAIVEVALRHPTDARVTTSQPALGTTANYELWIS
                     DGEVVRTYSAPHRLGTERPVRRSVVGISGRESRDLPGTSRVYAPLTALPMETLPEVFV
                     HPAGYCQNVLSTGACRIVGLDAVGGREAIVVECDHPRTIEIGADRPDYRIRISVDRAD
                     GVILRLEESIGGIVTRDARVTEYEPDAPLSPNTFDFTFPTGTTMLY"
     gene            complement(1916..3541)
                     /locus_tag="VHM48_06480"
     CDS             complement(1916..3541)
                     /locus_tag="VHM48_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2755089.1"
                     /translation="MAGSDTDRLHQMGYAQELRRGMRTFSNFAVSFTIISILSGCLTL
                     FKFGMLAGGPGVMTLGWLVVGFFVILVALGMAEVASSYPTAGGLYYWAAKLADEAGAN
                     GARWSWFVGWFNLVGQVAVTAGIDFGLSLFTQAFLNYLFGYPLDPPYLILVYGIMLSI
                     HALLNTFGIRIVALLNDLSVWWHLVGVAAIVLVTVVLNQHTRTDIGSVFTTFVDRTGS
                     TPEAPWPGPLLAGIPLYVMLIGLLNAQYTLTGYDASAHMSEETHDAARSAPKGIIYSV
                     VVSVIAGFILLVAMNVGITPDKAFQVTDATGAVTTLNGYDHAFGALVPPAQIWIDAVG
                     QAGGMIILFLVIGAQFFCGMSSVTANSRMIYAFARDGAIPASNFWHRINPKTRTPTNS
                     IWLAAIGAFILGLPYLWSPVAYFAITSIAVIGLYVAYVSPVFLRVRAGARFQTGPWTL
                     GRFGRPIGIVATIWVAIIVVLFMLPQYLPQVDDKGLVWANLNFTPVVFLVVLGGAAIW
                     YAVSAKNWFKGPRVQGTPEELAAIERDLDLAEG"
     gene            3693..4451
                     /locus_tag="VHM48_06485"
     CDS             3693..4451
                     /locus_tag="VHM48_06485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2755090.1"
                     /translation="MGDRLAGKVTIVTGAGSGIGRATAIRFAEEGARVTCVDIDAATV
                     GAVAAEIGNAAFAVAADVSDPRQVRAYTDATVERWGGLHAVFNNAGVNIPGVFHEVPD
                     EVVDRTLNVNVKGCIYGCRYAIPHMLRGGGGSLINTSSVNGLVAEPFLAIYATSKGAI
                     VMLSKGIALDYAKQGIRCNAIAPGWVDTPINYAHAEMLGGLSEVYATIDSFQPIGRPG
                     EPREIANVALFLASDESSFVTGSVIVADGGMTAK"
     gene            4505..>5727
                     /locus_tag="VHM48_06490"
     CDS             4505..>5727
                     /locus_tag="VHM48_06490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2755091.1"
                     /translation="MSPAKHGDHRATHANRDHPRLQELQAMVRDGRIDTLVVAITDMQ
                     GRLMGKRVQAQAFLEGVIDHGAHFCTYLLGTDMEMNTPEGFALMNWETGYGDWIAEPV
                     WDTLRVLPWLDKTALVLSDTFTDEDHQEIAVSPRTILKRQIARAADHGLTIKAGSEFE
                     YYVLKESWEESARLGWGIPQRFGYYNEDYHLLQATKAEPLHRLLRNQMTEARVPIEFS
                     KGEAAPGQHEVNIRYDHVLESADRSVIFKHGAKEIAWLNGWGITFMSKPDHTWTGSSG
                     HLHMSIWNEDGSKSLTFDPAAGRPYGMSDTFARFVAGMMALSRELSVFIAPNINSYKR
                     YASLSWAPVNIVWGRDNRTTGFRLVGHGPALHVEDRFPGGDMNAYLTYAAMIGAGLHG
                     IDQGLELVDEFKGNG"
BASE COUNT          992 a         1933 c         1975 g          827 t
ORIGIN      
        1 ggccgggcgc tcggcgagga ggtcgaggat ctccgcctcg taccgttcac gccagcgacg
       61 tgggtacagc cgaatgagcg aacgcatcag ccagctcctc caggcaccgc gccgatccgg
      121 atggcgaggt cggtcagaag atacgaccga cgtccatcgg aaccggccct cccttcgatc
      181 aggccgcgtc gctcgagccg ggcaatcgcg gcatagagcg tgcccggtcc gaccgggccg
      241 tcgagggcac gaacggcgtc gaacaggtgg accgcggtcc gaggcccatc gcgaagcacg
      301 gcgaggacaa gcctcgccgg ctccgcgaac cgcccgaacc cgcgggccac gatcatgtca
      361 gcgggccacg atcacgccgc atgcgatccg gccgccgctg ccgccattcg tcgcatcggt
      421 gagctggtcg tcttcattgg cgtggatgat gaacgagctg ccgaccgcgc cgctggtcgt
      481 gtcgaacagc gtggtcgggc cgttcttcag cgtcacccgg tcggttgtcg catcgaggtg
      541 tccgatcccg tcaccgttga cgatcaggtt cggcaggtcg cccgcgtggg cacccgacgt
      601 gttcaggagt ccgtgctggc gaacgagcgg gttgtagtgg ccgccggccg ccgcgaacgt
      661 gggcgtgcag gcagcgatcg aatggatgtg gatcccatgg agccccgggg tgaggccggc
      721 gacatggaca ttgacatgga cgatccccga ggcgtcctcg accaggcgag cccagccgac
      781 ggtcgcaccg gacgcgtcga cgatggtggc acccgcctgg gtcgccccgc cggcgagcga
      841 ctgggcggcg aatgccccca cgacggccaa ggcgaccccc agcatgccga tggacaggat
      901 gagtcgacgc aggacacgca tagccgggct ccctctactc gcaggatgat ataccgcctg
      961 tcgatatatc gcgttgcgat atatacgacg gcggcgccgc gcgtgtcaag accccggcta
     1021 caatcgccgc catgacctcg acccgcctct gggatcgcgc cgccgacctg ccgatggtgg
     1081 ccggtcgccg cccgcggccc gagatcgtcg cgctgcccgc cgccgggctg ccgaccgtgg
     1141 ccgagctgtt cacgttcatg cgcgatgccg agctgcgctt cgacgccctc cggatgcgga
     1201 tcgaggagcg cagcttcggc gttgccggcg agcaggtcgc gatcgtcgaa gtcgcccttc
     1261 gccacccgac cgacgcccgg gtcacgacga gccagccggc cctcgggacc accgccaatt
     1321 acgagctctg gatctccgac ggcgaggtcg tccggacgta cagcgctccc caccgcctgg
     1381 gcaccgagcg gccggtccgc cgcagcgtcg tcggcatcag cggccgcgaa tcgcgcgacc
     1441 tgcccgggac gagccgcgtc tacgcgccgc tgacggccct gccgatggag accctgccgg
     1501 aggtgttcgt ccacccggcg ggctactgcc agaacgtgct ctcgaccggg gcgtgccgga
     1561 tcgtcggcct cgacgcggtc ggcgggcgcg aggcgatcgt cgtcgagtgc gaccacccgc
     1621 ggacgatcga gatcggcgcg gatcgaccgg attaccggat ccggatctcc gtcgaccggg
     1681 ccgacggcgt gatcctccgc ctcgaggagt cgatcggcgg catcgtcacc cgggacgccc
     1741 gggtcaccga atacgagccg gacgcgccgc tcagccccaa cacgttcgat ttcacgttcc
     1801 cgaccgggac cacgatgctc tactgaccgc ctcgggcggt cgcgggtccg acgcccgtca
     1861 cgaatcggaa cgcccggccg gtggccgggc gttcgtcgtt gacggagacg gcgggtcagc
     1921 cctcggcgag gtccaggtcg cgctcgatcg cggccagctc ctccggggtg ccctggaccc
     1981 tcgggccctt gaaccagttc ttcgccgaca ccgcgtacca gatcgcagca ccgccgagga
     2041 cgacgaggaa cacgaccggg gtgaagttga ggttcgccca cacgaggccc ttgtcgtcaa
     2101 cctgcggcag gtactgcggc agcatgaaca ggacgacgat gatcgcgacc cagatcgtcg
     2161 cgacgatccc gatcgggcgg ccgaagcgtc cgagcgtcca ggggccggtc tggaaccgcg
     2221 ccccggcccg gacgcggagg aataccggtg agacgtacgc gacgtacagc ccgatgaccg
     2281 cgatcgaggt gatcgcgaag tacgcgaccg ggctccacag gtacggtagg ccgaggatga
     2341 aggccccgat ggcggcgagc cagatcgagt tcgtcggggt ccgggtcttg gggttgatcc
     2401 ggtgccagaa gttgctggcc gggatcgctc cgtcacgggc gaacgcgtag atcatccgcg
     2461 agttcgccgt gaccgacgac atgccgcaga agaactgggc gccgatgacg aggaacagga
     2521 tgatcatgcc gcccgcctga ccgacagcgt cgatccagat ctgggcaggc gggacgagcg
     2581 cgccgaacgc gtggtcgtat ccgttgaggg tcgtgaccgc gccggtcgcg tccgtcacct
     2641 ggaaggcttt gtccggggtg atgccgacat tcatcgcgac gaggaggatg aagcctgcga
     2701 tcaccgagac gaccaccgag tagatgatcc ccttcggcgc cgaccgcgcc gcgtcgtggg
     2761 tctcctcgga catgtgcgca gacgcgtcgt accccgtcag ggtgtattgc gcgttgagca
     2821 gaccgatgag catcacgtac agcgggatgc ccgccagcag tggaccgggc cacggtgcct
     2881 cgggagtgct gccggtccgg tcgacgaacg tcgtgaagac ggatccgata tcggtccggg
     2941 tgtgctggtt caggacgacc gtcacgagga cgatcgccgc gacgccgacg aggtgccacc
     3001 acaccgacag gtcgttgagg agcgcgacga tccggatgcc gaacgtgttc agcagggcgt
     3061 ggatcgagag catgatcccg tagacgagga tcaggtaggg gggatcgagc ggatagccga
     3121 acaggtagtt caggaaggcc tgggtgaaca gggagaggcc gaagtcgatg ccggccgtga
     3181 cggcgacctg gccgacgagg ttgaaccagc cgacgaacca gctccagcgg gcaccgttgg
     3241 cgccggcctc atccgcgagc ttcgcagccc agtaatacag gccgccggcg gtcggatagc
     3301 tcgacgcgac ctcggccatg ccgagcgcga cgaggatcac gaagaagccg acgaccagcc
     3361 agccgagcgt catcacgccg gggccgccgg cgagcatccc gaacttgaac agcgtcaggc
     3421 agcccgacag gatcgagatg atcgtgaagg agaccgcgaa gttggagaag gtccgcatcc
     3481 cgcggcgcaa ctcctgggcg taccccatct ggtggagtcg atcggtatcc gaacctgcca
     3541 tcccaatcct ccatgcgatg cacgtgacgg ttcgccggga cgcgagggac tcccccgccg
     3601 caacaatagg cgtcggggat cggcagtttc aaccgatcgc gacgcgccgc tcgcgatgac
     3661 ccgtctggcg cgccacggtc cggaggtggg cgatgggcga tcgactggcc ggcaaggtga
     3721 cgatcgtgac cggcgcgggt tccggcatcg ggcgggcgac ggcgatccgg ttcgcggagg
     3781 agggtgcccg ggtcacctgc gtcgacatcg acgcggcgac ggtcggggcg gtggccgcgg
     3841 agatcgggaa cgcagcgttc gccgtcgccg cggacgtctc ggatccacgc caggtccggg
     3901 cgtataccga cgcgaccgtc gagcgctggg gcggcctcca cgccgtgttc aacaacgccg
     3961 gggtcaacat cccgggcgtc ttccacgagg tcccggacga ggtcgtcgat cgaaccctga
     4021 acgtcaacgt caagggctgc atctacggct gccgctacgc gatcccccac atgctccgcg
     4081 gcggtggcgg atccctgatc aacacgagct cggtcaacgg cctcgtcgcc gagccgttcc
     4141 tggcgatcta cgcgacctcg aagggcgcga tcgtcatgct cagcaagggg atcgccctcg
     4201 actatgccaa gcagggcatc cgctgcaatg cgattgcccc gggctgggtc gacaccccga
     4261 tcaactacgc ccacgcggag atgctcggcg gcctgagcga ggtctatgcg acgatcgact
     4321 cgttccagcc gatcggtcgc cccggcgagc cgcgcgagat cgccaatgtc gcgctcttcc
     4381 tcgcctcgga cgagtcgtcg ttcgtcaccg gcagcgtcat cgtcgccgac ggcgggatga
     4441 ccgcgaagta gcgtccacca cgcagcgaag cagcgaggcc gcgagagcgg cgcggagggt
     4501 tcccatgtcc ccggcgaagc acggcgatca tcgcgcgacc cacgccaatc gcgaccaccc
     4561 gcggctccag gaactccagg cgatggtccg cgacggccgg atcgataccc tcgttgtcgc
     4621 gatcaccgac atgcagggcc ggctcatggg caagcgcgtc caggcccagg cgttcctcga
     4681 gggcgtcatc gatcacggcg cccatttctg tacctacctc ctcggcaccg acatggagat
     4741 gaacacgccc gagggcttcg ccctgatgaa ctgggagacg ggctacggcg actggatcgc
     4801 cgaacccgtc tgggacacgc tccgggtgct gccctggctc gacaagacgg cgctggtgct
     4861 gtccgacacg ttcaccgacg aggaccacca ggagatcgcg gtctcgccgc ggacgatcct
     4921 caagcgccag atcgccaggg ccgcggacca cggcctgacg atcaaggcgg gctcggagtt
     4981 cgagtactac gtcctcaagg agtcgtggga ggaatcggcc aggctcggct gggggatccc
     5041 ccagcggttc ggctactaca acgaggacta ccacctcctc caggcgacga aggccgagcc
     5101 tctccatcgg ctgctccgca accagatgac ggaggcgcgg gtcccgatcg agttcagcaa
     5161 gggcgaggcg gcgccgggcc agcacgaggt caacatccgt tacgaccacg tcctcgaatc
     5221 ggccgaccgg agcgtgatct tcaagcacgg ggccaaggag atcgcctggc tcaacggctg
     5281 gggcatcacc ttcatgtcga agcccgacca cacctggacg ggctcatcgg gccatctcca
     5341 catgagcatc tggaacgagg atggatcgaa gtcgctgacg ttcgatccgg ccgcggggcg
     5401 gccgtatggc atgtcggata cgttcgcccg tttcgtcgcc gggatgatgg ccctgagccg
     5461 ggagttgtcg gtcttcatcg ccccgaacat caactcctac aagcgctacg cgtcgctgtc
     5521 ctgggcgccg gtcaacatcg tgtggggccg cgacaaccgg acgacgggct tccggctggt
     5581 cggccacggg ccggcgctcc atgtcgagga ccgcttcccg ggcggcgaca tgaacgccta
     5641 cctgacgtac gccgcgatga tcggggcggg gctgcacggg atcgaccagg gcctggagct
     5701 cgtcgacgag ttcaagggca atggcta
//
LOCUS       DATAIW010000204        14059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_40407, whole genome shotgun sequence.
ACCESSION   DATAIW010000204 DATAIW010000000
VERSION     DATAIW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14059
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40407"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1233)
                     /gene="rlmN"
                     /locus_tag="VHM48_06495"
     CDS             complement(19..1233)
                     /gene="rlmN"
                     /locus_tag="VHM48_06495"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX2755092.1"
                     /translation="MPELRIAPDPSAPLRDPRPGLSGLASGELAAWFADRGEPAYRAR
                     QVGDAVWGRTATSVEDIRTLPVALREAVEAAFRFDTVGDTELRLSDGGLTEKGLHRLS
                     DGTLIESVLMHYPARAGQRERHTLCISSQGGCAVGCPFCATGELGFGRDLDTAEIVDQ
                     VRHAARRLAVDGKRLTNVVFMGMGEPLLNLDRVLAAVDALNDPRRFGLGARHITVSTS
                     GVVPGIRRLTALGPQFTLAVSLHAARDPLRDVLVPLNRRWTVAEVVAAAREHAAATGR
                     RVTYEATMIDGINDTETDAHAMAELLRGDHAHVNLIPMNPVAHTPWSASPDAIIERFA
                     AILAAAGVTATIRRNRGAEIGAACGQLAAERAGEPAPAVVARRRARLVDESAHALRGE
                     RSDEPVPAGVGE"
     gene            complement(1243..2310)
                     /gene="fmt"
                     /locus_tag="VHM48_06500"
     CDS             complement(1243..2310)
                     /gene="fmt"
                     /locus_tag="VHM48_06500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2755093.1"
                     /translation="MTDGAAEGDTDLRSAAEEHVRVRTVFLGSGRFAQPILGRLAAHP
                     AIDIVAVVTAPPRPVGRRQVETATPVESAARELGLEVLTPARLRDPAALADILGLEPA
                     LIVLADYGQIVPAALLDLPHGALNLHPSLLPRHRGATPIPAAILAGDLETGVSLMRMD
                     AGLDTGPLVAVEHVSLAGDETAPALEARLAIVAAGLLARVLDPWLAGELEPEPQSVDG
                     ASLTRPLRRRDGRIDPDLPAVLLERRVRAHLPWPGSFVEIDGDRLVVLDAAVTASDAD
                     DEPGRLVPDERGLALATRAGRLLLDEVQPAGGRPMSGEAFVRGRPSVLGRTVRRAPGV
                     ADDGRAVPNGSRSGDPSIEPA"
     gene            complement(2322..2861)
                     /gene="def"
                     /locus_tag="VHM48_06505"
     CDS             complement(2322..2861)
                     /gene="def"
                     /locus_tag="VHM48_06505"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2755094.1"
                     /translation="MSVRPIVLLGDPRLRLKGLPVDSFGRFLHELLGDMTDTMRHAPG
                     VGLAAPQVGEALQACVIEVRDTLYELVNPRIVRAAGDDRDLEGCLSLPGYYAYVTRSE
                     KVWVVAQNRNGKKIKVAGSGLLGRALQHELDHLEGKLFIDYLASMDELIPVGADDDET
                     DDGDDEHPTHTREAARALA"
     gene            complement(2989..3486)
                     /locus_tag="VHM48_06510"
     CDS             complement(2989..3486)
                     /locus_tag="VHM48_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755095.1"
                     /translation="MDETQNGPTGSNGPDPATDASPAGGSADETFGWTAQEDEAAAPG
                     GDARAAAERMLGQLQSMIDTVATQAAPVVRQIGAKAAELAAVAADRAGPLAHKAADAT
                     GHATEKLAERSRTWAADLRSKAADAAGGSADAEAASSASAAIDGADDATEETARDQPA
                     EGERV"
     gene            complement(3672..6182)
                     /gene="priA"
                     /locus_tag="VHM48_06515"
     CDS             complement(3672..6182)
                     /gene="priA"
                     /locus_tag="VHM48_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX2755096.1"
                     /translation="MTDQTAFDLGTDPRNGEPGEPGEPGAPLRHVEVAIDAAGGGGAR
                     RYTYAVPPSLANLEPGEAVLVEFGRRQALGVILGDAPPPDGIVAKPIAGRVRADGPLL
                     PPLALALAGWIADHYLAPPALVLRAMLPPGLLERLELLAERTAALPPPGLPAPDLDLL
                     DQLEHGPRPIRNLVAAEGRPALVRRLRAMADGGLVTLDWTLLAAGAGPRYERWIRLTA
                     AGRETARALAGDVRPAGRPLGPRQAAAMAELAEGPADGLPGAPLGDHHGTGALASLAR
                     RGLVEVDVRERPRRPLAQRPVGLRGGRPADADLTPPQAEAVALTRAAIAAGDPTPLLL
                     DGVTGGGKTAIYVEAIVASLERGRPALILVPEIALAMPLVDRLRSDLAVRVALVHSGL
                     GDGERADEWRRIRAGDVDIVVGTRLAILAPLADLGLIVVDEEHDAAYKSDRTPRIQAR
                     DVALRLAELAGAAIVLGSATPAVESIGRVREGRYRRVVLPSRPTGSRPVVEVVDLREE
                     LRAGNRGLLSGRLVEAIEALDRSAGDQAILTLNRRGTASVVLCRDCGHVQACPDCERP
                     LVYHQAGTTLRCHHCGRATPIATRCPNCRSPRIRYLGGGTQRVEEEVRRRFPELRVGR
                     LDRDVVERRGAAERVIDAFATGLLDVLVGTTLVTKGLDIPAVTLVGVVSADVALNLPD
                     ERAAERTYQLLAQAAGRAGRGDRPGLAIIQSYQPEHAAIRAVADDDGAAFYDSELALR
                     RRFGSPPFGRLVKLTVGIVDRDAAEREAAAMAERLRERARQRGADSAVLGPAPAYIAR
                     RADRWRWNLVLRGSDPASLLDGGLEPPWSVDVDPETLL"
     gene            complement(6235..6963)
                     /gene="gmk"
                     /locus_tag="VHM48_06520"
     CDS             complement(6235..6963)
                     /gene="gmk"
                     /locus_tag="VHM48_06520"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2755097.1"
                     /translation="MTDEPLALPADDALAASAALDADRHVSDDPGRPDYTVDGAPGAM
                     LIIISGPSGVGKDTIIDALRRHERERGLEDRRHYVVTVTTRAPRDGEVDGVDYHFVSR
                     DAFLRLRDDRGFLEANEVHGNWYGSPRDQVRAALATGKDVMLKIDVQGAQVVKEQVSQ
                     ALLIFVIPPSMSELFARLRARATETADELELRQRNAALEISRQRDYDKIVTNETGQVE
                     QTAAKINKIIAKEHRRHPTRRVRV"
     gene            complement(6980..7969)
                     /locus_tag="VHM48_06525"
     CDS             complement(6980..7969)
                     /locus_tag="VHM48_06525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX2755098.1"
                     /translation="MTPPGTHALQVPADALAQRVDRYVADVTGLSRSHVQKLISAGNL
                     TADGVPLKANAVIGPGTALQLIVPEPAPLDLLPEPDIPVRIVYEDADLLIIDKAAGLV
                     VHPSPGHSGGTLVNALLGLEGGGGFGGIAGVQRPGIVHRLDRDTSGLLMVARTDAAQH
                     SLMAQLKARRVKKTYLALVMGEVSAEVGRIEAPIGRDTKHRTKMAVVPDGRASTTGYR
                     VRERFRGWTLLELDLVTGRTHQIRVHLEAIGHPIAGDPVYGTGTSRKGPRVTGDAEPL
                     GRLFLHAWRLQLTSPSSGELIRAEAPLPDELEDVLEALRDDGTAALSGPEGRR"
     gene            complement(7966..8622)
                     /gene="lspA"
                     /locus_tag="VHM48_06530"
     CDS             complement(7966..8622)
                     /gene="lspA"
                     /locus_tag="VHM48_06530"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2755099.1"
                     /translation="MTDDSPTTAVGDRFVEPAPGASAAPRSARRRWPLFVGLAVAIVV
                     VDQLAKALVTRSLAPGQSVDVVGDLVRIVFGQNTGALFGMFKDNAAMFGVVSLVVVGL
                     IVAYHARSAASLYLTVTLGFLLGGAIGNMLDRLRLGFVVDFVDVGIGPTRFYTFNVAD
                     SAISLAILLLFIAAVRPSLVDGGARTSESPPPAAPTSTDEDWGDGLEPADRSPDEPAR
                     "
     gene            complement(8619..11975)
                     /locus_tag="VHM48_06535"
     CDS             complement(8619..11975)
                     /locus_tag="VHM48_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2755100.1"
                     /translation="MFRPVSAKPDLVAQEHEILAEWRERRTFERLRAQNAGGPHWSFL
                     DGPITANNPMGVHHAWGRAYKDLYQRFHAMLGQDQRYQNGFDCQGLWVEVNVERDLGF
                     TSKRDIEDYGIAEFVSLCKQRVLTFAARQTEQSIRLGMWMDWNDADELRRLRDLLAAD
                     PSATATIAGPDGPVSDTVEMLVGRLGMPDVGGSYFTFSNENNDLIWGFLKEVHRRGWL
                     YKGHDTMPWCARCGTGISQMEMNEGYADRDDPGLTVRLPLVDRPGEDLLVWTTTPWTL
                     TSNVAAAVGEGLRYVKVRQGEAIHWLGKGTMKQALAGPFEVLEERPGTDLVGWRYDGP
                     FDDLAAVRKAFAAGASAIAPDAPYEHRIVAWDDVGEEEGTGIVHIAPGCGAEDHTLGK
                     KLGLPMIAPLDDSGIVIDGFGSLTGRDVRDVTDPIIEHLKREGRFYRLETITHRYPHC
                     WRCQTPLVFRLVDEWYISMGPVYDRPRAELTKAEVDASLRYQIMETVDQIRWIPGFGY
                     DRELDWLINMHDWMISKKRYWGLALPIYDCKACGTFDVVGGREELHERAVDGWDRFDG
                     HTPHRPFIDEVTIACPSCGAPVERIRDVGNPWLDAGIVPFSTLHFREDPAFWEQWFPA
                     DFITESFPGQFRNWFYSMLAMSTVLRREPPFKSIFGYALVFGEDGRPMHKSWGNAIEF
                     DEAADRMGVDVMRWMFAKARPEENILFGWHAADEARRELLVLWNVYAFLVTYARLANW
                     TPGQGDATPPTRDRPALDRWIMSRTAGTAAEVEIRLGDYDAVGATRAISRLIDELSTW
                     YLRRSRKRFVHGGPDAASAFGTLDGTLVILARIMAPILPFMSETIYQNLVDPSIDERS
                     PDSVHLTKFPRNMEALRDEALEVSMAVVIRAVELVRTLRAQAGLRTRQPIAHLWLALP
                     GSAMVEREALLALLADEVNVKTIELIGDESALVERRVKPLLPKIGKKHGSAIPAIMAA
                     ARENAVEYHPDGSVTLGGVTLAPDEVEILASPRPGTAVAHDEGLVVVIDTELTDDLRA
                     EGDARELQRAIQDLRKEAGLALDDRIELWVDGLAESVAAHLDAVAEETLSDVVQRGAP
                     PTGPEITAGSIEIAGAPVGIAVRRIDGGAS"
     gene            12079..13908
                     /locus_tag="VHM48_06540"
     CDS             12079..13908
                     /locus_tag="VHM48_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2755101.1"
                     /translation="MQRAQEPRPRARSPFVAAVLSLLFPGLGHAYAGAYQRALGFAAP
                     PILIGALLAGVFLRMDAPKLLEFFLTNGVIQGIFIVNLVVLIYRLVAIVDAYRVTAFL
                     NAVATSGGGRLGRPRLVFNPLSVAGLLAAILIMSGGHVVVARYDLLANDVLDDPCVFI
                     SNDTDTPPSCDASPSASTSGSAGPTDVPLDSPTPSLSAPPVQGSALPAVTIPPWNGTD
                     RLNILLIGSDQRPNEGTYNTDTLIVVSIDPVTKQVAMFSLPRDSTDIPLPPGRLATAY
                     GGVYPNKINSLFTAVRGRPDLVAGTNATRGYNALKAVLGNLYQLDIKYFVEVNFDGFK
                     KVVDAMGGVTINVQVPVIDDSYPADSGRHARIYIPSGIQHMTGAQALIYARSRHGSDD
                     FDRGYRQQRVLTSLREQANIPNLIPRIPELYKAFRQTVRTDIPRAELAKLAGLAGSVG
                     TKDIRSYVFSFPRYGTQTPPSAPVYKYLPNVNRIRAAVANAFKVDPALEDTRAALGEE
                     HGTIWVLNGGSPVGMASSLAAYLEFYELTASAPSGKPDATGLTTTKIVVYNGAEARLP
                     ETIAFLEQKFKVKVQLKQDPAVSVDVIITTGSSTPNLAPPATN"
BASE COUNT         1950 a         5004 c         5076 g         2029 t
ORIGIN      
        1 agccagcacc ggccatcgct actcccccac cccggccggg accggttcgt cgctgcgctc
       61 gccgcggagg gcgtgggcgc tctcgtcgac gagccgcgcc cggcgccgcg cgacgacggc
      121 cggtgctggc tcgccggccc gctccgcggc gagctggccg caggctgccc cgatctccgc
      181 cccacggtta cgccggatcg tggccgtcac gccggccgct gcgaggatcg cggcaaaccg
      241 ctcgatgatc gcgtccgggg aggccgacca gggcgtgtgg gcgaccgggt tcatcgggat
      301 caggttgacg tgggcgtggt cgccgcgaag cagctcggcc atggcgtggg cgtcggtctc
      361 ggtgtcgttg atcccgtcga tcatcgtcgc ctcgtacgtc acccggcgcc cggtcgcggc
      421 ggcatgctcg cgggcggccg cgacgacctc ggccaccgtc cagcggcggt tgagcgggac
      481 gagcacgtcg cgcagcggat cgcgggcggc gtggagcgac acggccagcg tgaactgcgg
      541 gccgagcgcg gtcagccgcc ggatcccggg cacgacgccg gacgtggaca ccgtgatgtg
      601 gcgcgcgccg agcccgaacc gccgtgggtc gttgagggcg tcgaccgcgg cgaggacccg
      661 gtcgagattg aggagcggct cgcccatgcc catgaacacg acgttcgtca ggcgcttgcc
      721 gtcgaccgcg agacgtcggg ccgcgtggcg gacctggtcg acgatctcgg cggtgtcgag
      781 gtcccgcccg aagccgagct caccggtggc acagaacggg cagccgacgg cgcagccgcc
      841 ctggctcgag atacacagcg tatgccgctc gcgctggccg gcccgggcgg gatagtgcat
      901 gagcaccgac tcgatgagcg tcccgtcgga caggcggtgc aggcccttct cggtcaggcc
      961 gccgtcggac aggcggagct ccgtgtcgcc gacggtgtcg aagcggaagg ccgcctcgac
     1021 ggcctcgcgc agcgcgactg gcagcgtccg gatgtcctcg accgaggtcg ccgtccggcc
     1081 ccacacggcg tcgccgacct ggcgggcccg gtacgccggc tcgccacgat ccgcgaacca
     1141 ggcggcgagc tcgccgctcg caaggccgga caacccggga cgggggtcgc gaagaggagc
     1201 ggacgggtcg ggagcaatcc ggagttcggg cacggagcga gtctacgcgg gctcgatcga
     1261 tggatcgccc gaacgcgaac cgttcgggac cgcacgtcca tcgtcggcga cgcccggcgc
     1321 ccggcgaacg gtcctaccga gaaccgaggg tcgcccgcgc acgaacgcct cgccgctcat
     1381 cgggcggccg ccggcgggct gcacctcgtc gaggaggagg cgaccagccc gggtcgcaag
     1441 ggccaggcct cgctcatccg gaacgagccg gccgggctcg tcgtccgcgt ccgacgcggt
     1501 gacggccgca tcgagcacga ccaggcgatc cccgtcgatc tcgacgaagc tcccgggcca
     1561 cggcaggtgg gcgcgcaccc ggcgctcgag gagcaccgcc ggcaggtccg ggtcgatcct
     1621 cccgtcgcgg cgccgcagcg gccgggtcag gctggcgcca tccaccgatt gcggttccgg
     1681 ctcgagctcg cccgcgagcc acgggtcgag gactcgggcg agcaggcctg ccgcgacgat
     1741 cgcgaggcgc gcctccagag caggggcggt ctcgtcgccg gccagcgaca cgtgctcgac
     1801 cgcgacgagg gggccggtgt cgagacccgc gtccatccgc atgaggctca ccccggtctc
     1861 gaggtcgccg gccaggatcg cggcgggaat cggggtcgca ccacgatgcc gcggcagcag
     1921 cgacgggtga aggttcagcg cgccgtgcgg gaggtccagg agcgcggccg ggacgatctg
     1981 cccgtagtcg gcgaggacga tcagcgccgg ctcaagcccg aggatgtcgg cgagggcggc
     2041 gggatcgcgc agccgggcgg gcgtcaggac ctcgagaccg agctcgcggg cggccgactc
     2101 gacgggggtc gccgtctcga cctggcggcg ccccaccggt cgaggcggtg ccgtgacgac
     2161 ggccacgatg tcgatcgcgg ggtgggcggc gagacggcca aggatcggct gtgcgaagcg
     2221 gccgctaccc aggaagacgg tccggacgcg gacgtgctcc tccgcagccg aacgaaggtc
     2281 ggtgtccccc tcggccgcac cgtcggtcat cgacgggtcg atcacgcgag ggcccttgca
     2341 gcctcccggg tgtgggtcgg gtgttcgtcg tcgccgtcgt cggtctcgtc gtcgtcggca
     2401 ccgaccggga tgagctcgtc catcgaagcg agatagtcga tgaagagctt gccctcgaga
     2461 tgatcgagct cgtgctggag ggcccgcccg agcaggccag aaccggcgac cttgatcttc
     2521 ttgccgttcc ggttctgggc gacgacccag accttctcgc tgcgggtgac ataggcgtaa
     2581 tagcccggca gcgacaggca gccctcgagg tcgcggtcgt cgccggcggc acggacgatc
     2641 cgcgggttga ccagctcgta cagcgtgtcg cggacctcga tcacgcaggc ctggagggcc
     2701 tcgccgacct gcggcgcagc cagcccgacg cccggcgcgt ggcgcatggt gtcggtcatg
     2761 tcgccgagca gctcgtgaag gaacctgccg aagctgtcga cgggcaggcc cttcaaccgc
     2821 agccgcgggt ccccgaggag gacgatcggt cggacgctca tggccggcag tatagcgggg
     2881 ctcgggcggc cctccggccc gcccgatagg ccggccggtg gcctgtcgat ccggggcccg
     2941 gaaccggccc gcgccgaggc gacaggccgt cccggggccg attcccggtc agacgcgctc
     3001 gccctcggcc ggctgatcgc gggccgtttc ctcggtcgcg tcgtccgcac cgtcgatcgc
     3061 ggcgctggcg ctgctcgcag cctcggcatc agccgacccg cccgccgcat cggcggcctt
     3121 gctgcggaga tccgcggccc acgtgcgcga gcgctcggcc agcttctcgg tggcgtgccc
     3181 ggtcgcgtcc gccgccttgt gggccagggg gcccgcccga tccgcggcca ccgccgcgag
     3241 ctctgccgcc ttcgccccga tctggcggac gaccggcgcc gcctgggtcg cgaccgtgtc
     3301 gatcatcgac tggagctggc ccagcatccg ttccgcggcc gcgcgggcgt cgccaccggg
     3361 cgcggcggct tcgtcctcct gggcggtcca accgaaggtc tcgtcggcgc tgccgccggc
     3421 cggtgatgca tccgtcgcag ggtcggggcc gttcgatccg gtcggcccgt tctgtgtctc
     3481 gtccatgtcg tcctccgttc gtcgagtgcg ggtcctattg tcgcccggat cgggcggcca
     3541 cgctcagcga cgatctgcgc gagaaagtga cggcggctgt ctggatgccg cgcatcagtc
     3601 gcaggcggcg gctgttgtgc ccggttcccg tttcgggatc gcgcgagccg cgcgtcaggg
     3661 gcggatgcgg ctcagagcag ggtttcgggg tcgacgtcga ccgaccacgg cggctcgagc
     3721 ccgccgtcga ggaggctcgc cggatccgat ccgcggagga cgagattcca cctccagcgg
     3781 tcggcccgtc tggcgatgta cgccggggcc gggccgagga ccgcgctgtc cgcgccccgc
     3841 tggcgggccc gctcacggag acgttcggcc atcgccgccg cctcccgctc ggccgcgtcg
     3901 cgatcaacga tcccgacggt cagcttgacg aggcggccga acggcggtga gccgaagcgg
     3961 cggcgaaggg caagctcgct gtcgtagaag gccgcgccgt catcgtcggc gaccgcccgg
     4021 atcgcggcat gctcgggctg gtacgactgg atgatcgcga gcccggggcg gtcgccacgt
     4081 cccgctcgcc cggccgcctg ggcgaggagc tggtacgtcc gctccgccgc ccgctcgtcg
     4141 ggcaggttca gcgccacatc ggccgagacg acgccgacga gcgtgacggc cgggatatcg
     4201 agacccttcg tgaccagggt cgtcccgacg aggacgtcga ggaggcccgt cgcgaaggcg
     4261 tcgatgaccc gttccgcggc gccacgccgt tcgacgacgt cgcgatcgag gcgcccgacc
     4321 cgcagctccg ggaaccgccg ccggacctcc tcctcgaccc gctgggtgcc gccgccgaga
     4381 taacggatcc gcggcgatcg gcaattcggg cagcgggtcg cgatcggcgt ggcccggcca
     4441 cagtggtggc agcggagcgt cgttccggcc tggtggtaga cgaggggccg ctcgcagtcg
     4501 ggacaggcct ggacgtgccc gcaatcacgg cacaggacga ccgacgcggt cccccgtcgg
     4561 ttgagcgtca ggatcgcctg gtcgccggcg ctccggtcga gcgcctcgat cgcctcgacg
     4621 agccgtcccg agagcaggcc gcgatttccg gcccggagct cctcacggag gtccacgacc
     4681 tcgacgacgg gccggctgcc ggtcggacgc gacggcagga cgacccgccg ataccggccc
     4741 tcgcgcaccc ggccgatgct ctcgacggcg ggcgtggcac taccgaggac gatcgcggcg
     4801 ccggccagct cggccagccg gagggcgacg tcgcgcgcct ggatccgcgg cgtccggtcg
     4861 ctcttgtagg cggcatcgtg ctcctcgtca acgacgatca gcccgagatc ggcgagcggg
     4921 gcaaggatcg cgaggcgggt gccgacgacg atgtcgacgt cgcccgcccg gatccgccgc
     4981 cactcgtcgg cccgctctcc gtccccgaga ccggagtgga cgagggccac ccggaccgcc
     5041 aggtcggatc ggagccggtc gacgagcggc atcgcaaggg cgatctccgg gacgaggatg
     5101 agggccggcc ggccgcgctc gagcgacgcg acgatcgcct cgacgtagat cgcggtcttc
     5161 ccgccaccgg tcactccgtc gagcaggagc ggtgtcgggt cgccggccgc gatcgccgca
     5221 cgggtgagcg cgacggcctc ggcctggggc ggggtcaggt cggcgtcggc gggtcggcct
     5281 ccgcgaagcc cgaccgggcg ctgggcaagg ggccggcgcg ggcgctcgcg gacatcgacc
     5341 tcgacgaggc cgcggcgggc gaggcttgcc agggcgccgg tcccgtgatg gtcaccgagc
     5401 ggcgccccgg gcaggccgtc cgccgggccc tcggccagct ccgccatcgc cgccgcctgg
     5461 cgtggtccga gtggccggcc agccggccgc acgtcgcccg ccaacgcccg tgctgtctcg
     5521 cgcccggccg ccgtcagccg gatccagcgc tcgtagcgcg ggccggcccc agcggcgagc
     5581 agcgtccagt cgagggtgac gaggccgccg tcagccatgg cccgcaaccg ccggacgagg
     5641 gccggccgtc cctccgcggc gacgaggttg cggatcggtc gtggcccgtg ctcgagctgg
     5701 tcgagcaggt cgagatcggg tgccggaagt ccgggcggcg gcaacgcggc cgtccgctcg
     5761 gcgagcagct cgagccgctc cagcaagccc gggggcagca tcgcccgcag gaccagggcc
     5821 ggtggcgcca ggtagtgatc ggcgatccag ccggcgagcg cgagggcgag cggcgggagg
     5881 agcggtccgt ccgcccggac ccgtcccgcg atcggcttgg cgacgatccc gtcgggcggt
     5941 ggcgcgtcgc cgaggatgac gccgagcgcc tgacgccgcc cgaactcgac gaggacggct
     6001 tcgccgggct cgagattcgc aagcgaaggc ggtacggcgt acgtgtagcg cctggcgcca
     6061 ccgcccccgg cggcgtcgat cgcgacctcg acgtggcgga ggggcgcgcc gggctcgccg
     6121 ggctcgccgg gctcgccgtt gcgaggatcg gtccccaggt cgaaggcggt ctggtctgtc
     6181 atgacgcggt ggggctccgc tcgcggtccg gacggacggc ggtcccggcg ctcgctagac
     6241 ccggacgcgg cgggtcgggt ggcgccgatg ctccttggcg atgatcttgt tgatcttcgc
     6301 cgccgtctgt tcgacctgtc cggtctcgtt ggtgacgatc ttgtcgtagt cccgttgtcg
     6361 ggagatctcg agggcggcgt tccgctggcg cagctcgagc tcatcggcgg tttcggtcgc
     6421 cctcgcccgg agccgtgcga acagttcgct catcgatggt ggaatgacga agatcaggag
     6481 cgcctggctg acctgctcct tgacgacctg ggcaccctgg acgtcgatct tgagcatcac
     6541 gtccttgccg gtcgcgagcg ccgcccggac ctgatcgcgg ggcgagccat accagttgcc
     6601 gtggacctcg ttcgcctcga ggaagccgcg gtcgtcgcgg agccggagga acgcgtcacg
     6661 gctgacgaag tggtaatcga cgccgtcgac ctcgccgtcc cgcggtgcgc gggtcgtgac
     6721 cgtcacgaca tagtgacggc gatcttcgag ccctcgctcg cgctcgtgcc gacggagggc
     6781 gtcgatgatc gtgtccttgc cgaccccgga cggtcccgag atgatgatca gcatcgcccc
     6841 cggagcgccg tcgacggtgt agtcgggccg ccccgggtcg tccgagacgt ggcggtcggc
     6901 gtcgagggcc gccgacgcgg cgagggcgtc gtcggccggc agggccagcg gctcgtcggt
     6961 cactggacta ccctcgcggt catcggcgcc cctccggtcc cgagagggcc gcggtcccgt
     7021 cgtcgcgcag cgcctcgagc acgtcctcca gctcgtccgg cagcggcgcc tccgcccgga
     7081 tcagctcgcc gctgctcggc gaggtcagct gcagccgcca ggcgtgcagg aacagccgtc
     7141 cgagcggctc ggcatcgccg gtgacccgcg ggcccttccg cgaggtgccc gtgccgtaca
     7201 cgggatcgcc cgcgatcggg tggccgatcg cctcgaggtg gacccggatc tggtgggtcc
     7261 gcccggtgac gaggtccaac tcgagcagcg tccagccgcg gaatcgctcg cggacgcggt
     7321 agccggtcgt cgacgcccga ccgtcgggga cgacggccat cttcgtccgg tgcttcgtgt
     7381 cgcgcccgat cggcgcctcg atccggccga cctccgccga cacctcgccc atcacgaggg
     7441 cgaggtacgt cttcttgacc cgccgcgcct tcagctgggc catgagcgag tgctgggcgg
     7501 cgtccgtccg ggcgaccatc agcaggccgc tcgtgtcgcg gtcgaggcga tggacgatgc
     7561 ccggtcgctg gacgccggcg atcccgccga acccaccgcc gccctccagg ccgaggaggg
     7621 cgttgacgag ggtcccgccg ctgtgacccg gcgacggatg gacgacgagc ccggcggcct
     7681 tgtcgatgat caggaggtcg gcgtcctcgt acacgatccg gaccgggatg tccggctcgg
     7741 gaaggaggtc gagcggcgcg ggctccggga cgatcaactg gagcgcggtc ccgggcccga
     7801 tcaccgcgtt ggccttgagc gggaccccgt cggcggtcag gttgcccgcg ctgatcagct
     7861 tctggacgtg gctccgtgac aggccggtga cgtccgcgac atagcgatcg acgcgttggg
     7921 cgagtgcatc ggccggaacc tgcagggcgt gggtaccggg cggggtcatc gggccggctc
     7981 gtcgggcgag cggtcggcgg gctcgagacc gtcaccccag tcctcatcgg tgctggtggg
     8041 cgccgcgggc gggggtgatt cgctggtccg cgccccgccg tcgacgaggg acggccggac
     8101 ggcagcgatg aacaggagga ggatcgcgag gctgatcgcg ctgtcggcca cgttgaacgt
     8161 gtagaaccgg gtcgggccga tcccgacgtc gacgaagtcg accacgaagc cgaggcgcag
     8221 gcggtcgagc atgttgccga tcgccccgcc gagcaggaac ccgagcgtca ccgtcaggta
     8281 gaggctggcc gccgaccggg cgtgataggc gacgatcagc ccgacgacga cgagcgagac
     8341 gacgccgaac atcgcggcat tgtccttgaa catcccgaac agggcgcccg tgttctggcc
     8401 gaacacgatc cggacgaggt cgcccacgac gtcgaccgat tggccagggg cgagggagcg
     8461 ggtcacgagg gccttcgcca gctggtcgac gacgacgatc gccacggcga ggccgacgaa
     8521 caggggccag cgccggcggg cgctccgcgg cgccgccgac gctccgggtg ccggctcgac
     8581 gaaccgatcg ccgaccgcgg tcgtcgggct gtcgtcggtc acgacgctcc cccgtcgatc
     8641 cggcggacgg cgatcccgac gggggccccg gcgatctcga tcgaaccggc ggtgatctcc
     8701 gggccggtcg gcggcgcgcc gcgctggacc acgtcggaaa gcgtctcctc ggcgacggcg
     8761 tcgaggtggg ccgcgaccga ctcggcgagt ccgtcgaccc acagctcgat ccggtcgtcg
     8821 agggccaggc ccgcctcctt gcggagatcc tggatcgccc gctggagctc gcgcgcatcg
     8881 ccctcggcgc ggaggtcgtc ggtcagctcc gtgtcgatga cgacgaccag gccctcgtcg
     8941 tgggcgacgg cggtgccggg tcgcggcgac gccaggatct cgacctcgtc aggcgcgagg
     9001 gtgacgccgc cgagcgtgac cgagccgtcg gggtggtact cgaccgcgtt ctcgcgggcg
     9061 gccgccatga tcgcggggat cgccgatccg tgcttcttgc cgatcttcgg cagcagcggc
     9121 ttcacccgcc gctcgaccag cgccgactcg tcgccgatga gctcgatcgt cttgacgttg
     9181 acctcgtccg cgaggagcgc caggagagcc tcgcgttcga ccatggcact ccctggcagg
     9241 gcaagccaga ggtgagcgat cggctggcgc gttctgaggc cggcctgcgc gcgcagggtc
     9301 cgaacgagct cgaccgctcg gatcacgacg gccatcgaga cctcgagcgc ttcgtcccgg
     9361 agagcctcca tgtttcgcgg gaacttcgtc aggtggacgc tgtccggcga gcgttcgtcg
     9421 atcgatggat ccacgaggtt ctggtagatc gtctcggaca tgaagggcag gatgggggcc
     9481 atgatccggg cgagaatgac gagcgtgcca tcgagcgtac cgaaggccga ggcggcgtcg
     9541 ggcccgccgt gcacgaagcg cttccgcgat cgccggaggt accacgtcga gagttcgtcg
     9601 atcaggcggc tgatcgctcg cgtggccccc accgcgtcgt agtccccgag tcgtatctcg
     9661 acctcggcgg cggtacccgc cgtccgcgac atgatccaac gatcgagcgc cggccgatcc
     9721 cgggtcggcg gcgtggcgtc gccctgacct ggcgtccagt tggcgagtcg cgcatacgtg
     9781 acgaggaacg cgtagacgtt ccacaggacg aggagctcgc gtcgcgcctc gtccgccgca
     9841 tgccagccga agaggatgtt ctcctcgggc cgggcctttg cgaacatcca gcgcatgacg
     9901 tcgacgccca tccgatcggc cgcttcgtcg aactcgatcg cgttgcccca gctcttgtgc
     9961 atcggccggc cgtcctcgcc gaagacgagg gcgtagccga agatcgactt gaacggcggt
    10021 tcgcggcgga ggaccgtgga catcgcgagc atcgagtaga accagttgcg gaactggccg
    10081 ggaaagctct cggtgatgaa gtcggccggg aaccactgct cccagaacgc cgggtcctcg
    10141 cggaaatgga gggtcgagaa cggcacgatc cccgcgtcca gccacgggtt gccgacgtcc
    10201 cggatccgct cgaccggcgc cccgcacgat gggcaggcga tcgtcacctc gtcgatgaac
    10261 ggccggtgcg gggtgtgccc gtcgaaccgg tcccaaccgt cgaccgctcg ttcgtggagc
    10321 tcctcgcggc cgccgacgac gtcgaacgtc ccgcacgcct tgcagtcgta gatcggcagc
    10381 gccaggcccc agtagcgctt cttgctgatc atccagtcgt gcatgttgat cagccagtcg
    10441 agctcgcggt cgtagccgaa gcccgggatc cagcggatct ggtcgacggt ctccatgatc
    10501 tggtagcgga ggctggcgtc gacctcggcc ttcgtcagct cggcccgcgg ccggtcatac
    10561 accggaccca tcgagatgta ccactcatcg acgaggcgga agacgagcgg cgtctggcag
    10621 cgccagcagt gcggatagcg gtgggtgatc gtctccagcc ggtagaagcg gccttcgcgc
    10681 ttgagatgct cgatgatcgg gtcggtcacg tcccggacgt cgcgcccggt cagcgacccg
    10741 aagccgtcga tcacgatccc gctgtcgtcg agcggcgcga tcatcggcag gccgagcttc
    10801 ttgccgaggg tgtggtcctc cgcgccgcag cccggcgcga tgtggacgat cccggtcccc
    10861 tcctcctcgc cgacgtcgtc ccaggcgacg atccggtgct cgtacggggc atcgggcgcg
    10921 atcgctgagg cgccggcggc gaacgccttg cggaccgccg ccaggtcgtc gaacggtccg
    10981 tcgtaacgcc agccgacgag atccgttccg ggacgctcct cgagcacctc gaacggaccc
    11041 gcgagggcct gcttcatggt ccccttgccg agccagtgga tcgcctcgcc ctggcggacc
    11101 ttgacatacc gcagcccttc cccgacggcg gctgcgacat tcgaggtcag cgtccagggg
    11161 gtcgtggtcc agaccaggag atcctcgcct ggccggtcga cgagcggaag gcggaccgtc
    11221 agcccggggt cgtcgcggtc ggcgtagccc tcgttcatct ccatctgcga gatgcccgtc
    11281 ccgcagcgag cgcaccacgg catcgtgtcg tggcccttgt agagccagcc gcgacggtgg
    11341 acctccttga ggaagcccca gatgaggtcg ttgttctcgt tgctgaacgt gaagtaactg
    11401 ccgccgacgt ccggcatccc gagccgcccg acgagcatct cgaccgtgtc gctgaccggg
    11461 ccgtccggac ccgcgatcgt ggccgtcgcg ctgggatccg cggcgagcag gtcacgcagc
    11521 cggcgcagct cgtccgcgtc gttccagtcc atccacatcc cgagtcggat gctctgctcg
    11581 gtctggcggg cggcgaaggt caggacccgc tgcttgcaga gcgagacgaa ctcggcgatc
    11641 ccgtagtcct cgatgtcgcg cttgctggtg aagccgaggt cccgctcgac gttgacctcg
    11701 acccacagcc cctggcagtc gaagccgttc tggtagcgct ggtcctggcc gagcatcgcg
    11761 tggaagcgct ggtacagatc cttgtaggcg cggccccaag cgtgatggac gcccatcggg
    11821 ttgttggccg tgatcgggcc gtcgaggaag ctccagtgcg gaccgccggc attctgggca
    11881 cggaggcgct cgaacgtccg acgctcgcgc cactcggcaa ggatctcgtg ctcctgcgcg
    11941 acgaggtcgg gtttcgcgct gacgggtcgg aacatcgggc ctcggctggg gtgatcccgg
    12001 ggacggtcgg cgtcggcgcg ttgggaacgc gggcggcctc gacgacggcc gacgggtctc
    12061 cgagtatcct accctgcgat gcagcgcgcg caggaaccgc ggccgagggc aagatcgccc
    12121 ttcgtcgccg ccgtcctctc cctcctcttt ccgggcctcg gtcacgcgta cgccggggcc
    12181 taccagcgcg ccctcgggtt cgcggcgccg ccgatcctga tcggggcgct cctcgccggc
    12241 gtcttcctgc ggatggacgc cccgaagctg ctcgagttct tcctgaccaa cggggtcatc
    12301 cagggcatct tcatcgtcaa cctcgtcgtc ctgatctacc gactcgtcgc gatcgttgac
    12361 gcctaccgcg tcacggcctt tctcaacgct gtcgcgacga gcggcggcgg gcgcctcggg
    12421 cggccgcggc tcgtcttcaa cccgctctcg gtcgcgggcc tcctcgccgc gatcctgatc
    12481 atgtcgggcg gccatgtcgt tgtcgcccgc tacgaccttc tcgccaacga cgtcctcgac
    12541 gatccgtgcg tcttcatctc gaacgacacc gacaccccgc ccagctgcga tgcctcgccg
    12601 tcagcctcga cgtccggctc ggccgggccg accgacgtgc ccctcgattc gccgacgcca
    12661 tcgctgtcgg caccgccggt ccaggggtcg gccctgccgg cggtcacgat tccgccgtgg
    12721 aacggcaccg accggctcaa catcctgctg atcggctcgg accagcggcc gaacgaaggc
    12781 acctacaaca ccgacaccct gatcgtcgtg tcgatcgacc cggttaccaa gcaggtggcg
    12841 atgttcagcc tgccgcgtga ctcgaccgac atcccgttgc cgcccgggcg gctggcgact
    12901 gcgtatggtg gggtctatcc caacaagatc aattcgctgt tcaccgccgt ccgcggtcgg
    12961 cccgacctgg tcgccgggac gaacgcgacg cggggttaca acgcgctcaa ggccgtcctc
    13021 ggcaacctct atcagctcga catcaagtac tttgtcgagg tcaacttcga cggcttcaag
    13081 aaggtcgtcg acgcgatggg cggggtgacg atcaacgtcc aggtgcccgt catcgacgac
    13141 agctacccgg ccgattccgg tcgccacgcc cggatctaca tcccgtccgg catccagcac
    13201 atgaccggcg cccaggccct gatctacgcc cgatcgcgcc acggctccga cgacttcgac
    13261 cgcggctacc gccagcagcg cgtcctcacc tcgctccgcg agcaggccaa catcccgaac
    13321 ctcatcccgc ggattcccga gctgtacaag gccttccggc agacggtccg gacggacatc
    13381 ccgcgggcgg agctggccaa gctggcggga ctcgccggca gcgtcggcac caaggacatc
    13441 cgctcctatg tgttctcgtt cccgcgctac gggacgcaga ccccgccgag cgcgccggtc
    13501 tacaagtacc tgccgaacgt caacaggatc cgggccgcgg tcgccaacgc gttcaaggtc
    13561 gatccggcgc tcgaggacac ccgggccgcc ctcggtgagg agcacggcac gatctgggtc
    13621 ctcaacggcg gcagcccggt cgggatggcc agctcgctcg cggcatatct cgagttctac
    13681 gagttgacgg catccgcgcc ctccgggaag ccggacgcga ccggcctgac gaccacgaag
    13741 atcgtcgtct acaacggtgc cgaggcacgc ctcccggaga cgatcgcgtt cctcgagcag
    13801 aagttcaagg tcaaggtcca gctgaaacag gacccggccg tgtcggtcga cgtgatcatc
    13861 acgaccgggt cgtcgacccc gaacctcgcc ccgcccgcga cgaactgagg tctcccgcca
    13921 gggtcgatgg ccgtcgcgcc tttgcggccg gtcgcggcgt tctccctgac tcagtggctg
    13981 agtcagatca gcggtgagcg cgctcggcat cgtcggagtt gcgaccgagc cacgcacgct
    14041 gaagcccgcg ctgactcgc
//
LOCUS       DATAIW010000205         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_242924, whole genome shotgun sequence.
ACCESSION   DATAIW010000205 DATAIW010000000
VERSION     DATAIW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242924"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VHM48_06545"
     CDS             complement(<1..799)
                     /locus_tag="VHM48_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2755102.1"
                     /translation="MDPKSRFYTGTRGGLAVRAFVVAGPGRAAVEEVEPPAATPGQVV
                     IDVERVGLCGTDAEFFTGDMAYLHQGHARYPLRLGHEWSGTVSAVGEGVDPGWIGRRT
                     TGDTMLGCGGCRRCLAGRAHLCEDRFEVGIRRGWAGALAERMAVPATSLHGLPDAVDP
                     VAGALVEPGANALRAVLAAAVGRGGRLLILGGGTIGLLAAQLAVADGGEVHLMGRSAR
                     SLDFARELGLDRVWRLETLPRLPFDAVIDASNAPDLPALALDLVEPGG"
     gene            822..2537
                     /locus_tag="VHM48_06550"
     CDS             822..2537
                     /locus_tag="VHM48_06550"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX2755103.1"
                     /translation="MPTRRSSQWYAGTDRNAFIHRAWMRRGLPADAFDGRPHIAIANT
                     ASDLTPCNAHLDEVAASVKEGIWEAGGIPLNLPVVSLGETQVRPTAMLWRNMAAMAIE
                     EMLRANPIDGVVLLGGCDKTIPALLMAAASVDLPAVLIPGGPMLSGTFRGVALGCGTD
                     VWKLSEEVRAGGLSEQEFLQSESSMIRSRGHCNTMGTASTMAVVAEALGMTIPGLAGT
                     PAPDSRLLEASHATGRLAVELVEQDRRPSAIVSRGSFRNAIVALAAVGGSTNAVIHLL
                     AIAGRLGVELTLDDFDRIGAGVPLLVDLQPAGRFLMDDLHRAGGLLAVLREVRELLDP
                     DAMTVTGRPLAEHLADARIWDEEVIRPRASPLLADAGIAVLRGNLAPDGAVIKPAAAS
                     PELLRHRGRVLVFDSIEDFNARIDDPRLDVDANTVLVLRGCGPRGYPGMPEVSNMPLP
                     AKLLTQGVRDMVRICDGRMSGTAYGTVVLHVAPEAAAGGPLGLVETGDVIVLDVPARR
                     VDVDVPQTELDGRRRSPEMVARFAAPTRGWERLYVDHVLQADTGADLDFLLGSSGDRV
                     ARDSH"
     gene            2564..>2828
                     /locus_tag="VHM48_06555"
     CDS             2564..>2828
                     /locus_tag="VHM48_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755104.1"
                     /translation="MNCRTDGRAEIVPVVESYPAPPLAETSRDHRVRVLHQRVFVERY
                     ADVSYARFAMRGPVTVEIEVESPIASHTIFPAERVAAPSVDGNV"
BASE COUNT          390 a         1032 c         1026 g          380 t
ORIGIN      
        1 gcccgccggg ctcgacgagg tcgagcgcga gcgcaggcag gtcgggcgcg ttcgacgcgt
       61 cgatgaccgc gtcgaatggg agccggggca gcgtctcgag acgccagacg cggtcgagcc
      121 caagctcgcg ggcgaagtcg agcgaccgcg cggatcggcc catgaggtgg acctcgccgc
      181 cgtccgcgac agcgagctgc gcggccagga gcccgatcgt gccgcccccg aggatgagca
      241 ggcggccgcc ccgcccgacg gcggccgcca ggaccgcccg gagcgcgttc gcgccgggct
      301 cgacgagggc accggcgacc gggtcgaccg cgtcgggcag tccgtggagc gatgtcgccg
      361 ggaccgccat ccgctccgcg agggcgccgg cccagccccg gcggatcccg acctcgaagc
      421 ggtcctcgca gaggtgcgcc cgcccggcga ggcagcgccg gcacccgccg caaccgagca
      481 tcgtgtcgcc cgtcgtccgg cggccgatcc agccggggtc gacgccctcg ccgaccgccg
      541 acaccgttcc ggaccactcg tggccgaggc gcagcgggta gcgggcgtga ccctggtgga
      601 gataggccat gtcaccggtg aagaactcgg cgtccgtgcc gcacagaccc acacgctcga
      661 cgtcgatgac gacctggccc ggcgtggcgg cgggcggctc gacctcctcg accgcggcac
      721 ggcccggccc cgcgacgacg aaggccctca ccgcgagccc gccgcgggtt ccggtgtaga
      781 accggctctt cggatccatc tcattcggcg aatataggtc agtgccgact cgccgcagct
      841 cgcagtggta cgcgggaacc gaccgcaacg ccttcatcca tcgggcgtgg atgcgccgcg
      901 gactgccggc cgacgccttc gacggccggc cgcacatcgc gatcgcgaac accgcgtccg
      961 acctgacgcc gtgcaatgcc catctcgacg aggtcgctgc gagcgtcaag gaggggatct
     1021 gggaggccgg cggcatcccc ctgaacctgc ccgtcgtgtc gctcggcgag acgcaggtcc
     1081 ggccgacggc aatgctctgg cggaacatgg ccgcgatggc gatcgaggag atgctccggg
     1141 ccaacccgat cgacggggtc gtcctcctcg ggggctgcga caagacgatc ccggccctcc
     1201 tcatggcggc cgcctcggtc gacctgccgg ccgtcctcat tcccggcggt ccgatgctga
     1261 gcggcacgtt ccggggagtc gcgctcggct gcgggacgga cgtctggaag ctcagcgagg
     1321 aggtccgcgc cggcgggctg tcggagcagg agttcctgca gtccgagtcc tcgatgatcc
     1381 gcagccgcgg ccactgcaac acgatgggca cggcctcgac gatggcggtg gtcgcggagg
     1441 ccctcgggat gacgatcccg ggcctggccg gcacgcccgc ccccgacagc cggctgctcg
     1501 aagcgtcgca cgccacgggg cgcctggccg tcgagctggt cgagcaggat cgccggccga
     1561 gcgcgatcgt cagtcgcggc tcgttccgca acgcgatcgt cgcgctggcc gcagtcggcg
     1621 ggtcgacgaa tgcggtcatc cacctgctcg cgatcgccgg ccggctcggg gtcgagctga
     1681 ccctcgacga cttcgaccgg atcggggccg gcgtgccgct cctcgtcgat ctgcagccgg
     1741 ccggccgatt cctgatggac gatctccatc gggccggcgg cctcctggcc gtcctgcggg
     1801 aggtccggga gctgctcgat ccggacgcca tgaccgtgac cgggcgaccg ctcgcagagc
     1861 acctggccga tgcccggatc tgggacgagg aggtcatccg gccccgggcg agcccgctcc
     1921 tggccgatgc cgggatcgcg gtcctgcgcg gcaacctggc gcccgatggt gcggtcatca
     1981 agccggccgc cgcctcaccg gagctgctcc ggcatcgcgg acgagtcctc gtgttcgact
     2041 cgatcgagga cttcaacgcc cggatcgacg acccgcgcct cgacgtggat gcgaataccg
     2101 tgctcgtgct gcgcggctgc ggtccgcggg gctacccggg gatgccggag gtctccaaca
     2161 tgccgctgcc ggcgaagctc ctgacccagg gcgtccgcga catggtccgg atctgtgacg
     2221 gccggatgag cgggaccgcg tacggcacgg tcgtgctgca cgtggcgccc gaggcggccg
     2281 cgggcgggcc gctcggcctc gtcgagaccg gcgatgtcat cgtcctggac gtgccggccc
     2341 gtcgagtgga cgtggacgtc ccgcagacgg agctcgacgg acgccggcgg tcgccggaga
     2401 tggtcgcacg cttcgccgcc ccgacccgtg gctgggagcg cctgtatgtc gaccacgtcc
     2461 tccaggcgga cacgggtgcg gacctcgatt tcctgctcgg ctcgagcggc gaccgcgtcg
     2521 cgcgcgattc gcactgatcg ctggggccag gtcgcccgtc accatgaatt gcaggactga
     2581 tggacgggcg gaaatcgttc ccgtcgtcga gagctacccg gcgccgccgc tcgccgaaac
     2641 gagccgcgac caccgcgtgc gcgtcctcca ccagcgggtg ttcgttgagc gctacgccga
     2701 cgtgagctat gcccggttcg ctatgcgcgg gccggtcacg gtcgagatcg aggtcgagag
     2761 cccgatcgcg agccacacga tcttccccgc cgagcgcgtt gccgcgccca gtgtcgacgg
     2821 caatgtcc
//
LOCUS       DATAIW010000206         7119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183485, whole genome shotgun sequence.
ACCESSION   DATAIW010000206 DATAIW010000000
VERSION     DATAIW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183485"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..435)
                     /locus_tag="VHM48_06560"
     CDS             complement(250..435)
                     /locus_tag="VHM48_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755105.1"
                     /translation="MRPPRPAAQPVNEPLKHVIAEYKHQAQTITCVCGWHGKSSSPDG
                     RTSEWTTHLAANRLKPR"
     gene            complement(612..1193)
                     /locus_tag="VHM48_06565"
     CDS             complement(612..1193)
                     /locus_tag="VHM48_06565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2755106.1"
                     /translation="MPTRLVLARHGETAWSRAGQHTGRTDIPLTDVGREQARRLGAVL
                     AGRSFSRVFSSPLSRASETARLAGFDTGVEIDPELQEWDYGIYEGRRRVDIARDDPGW
                     TIWSRPIPGGESLAALGRRVDRVIARLLPLGGDVLVFSHGHFLRVLAARWIEAPPVTA
                     SRLELWTATVSELGWEADRRVVEIWNATAHPAG"
     gene            complement(1232..1816)
                     /locus_tag="VHM48_06570"
     CDS             complement(1232..1816)
                     /locus_tag="VHM48_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX2755107.1"
                     /translation="MTGLAAAFAVGVVVAIVLFVVVATYNDVVALQRRIDKAWANIEV
                     ALQQRFDQLPALVDAVRGVMTFERDVLAEVTAARAAFSPTAPIPDQAATSAATTRAVR
                     SLFATVERYPEIKSATNVLALQSEIERLESMIADRRELYNDQVYRYNTRIAQLPGVLL
                     ASPFGWTARVFFDADPAADRPPSSLTDGEAEPGA"
     gene            complement(1813..2895)
                     /locus_tag="VHM48_06575"
     CDS             complement(1813..2895)
                     /locus_tag="VHM48_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755108.1"
                     /translation="MIHVPLPPGTVFAGLAAVGVVAGLVVLIRGFGGYRAAGRISGTS
                     VSRIASLAVGEVLVSGTAEAIELTLISPLQSIPCVYYRSRITETRDDNQRDLFKEDRA
                     VGFRVRDASGAVRIFPNGARFDVPDRYDESTSSWSGGPVGLQPRTGPAYAPGPEDREA
                     QIAALLTVKAPLNWSLLDRAGLPLAATGSQAHYREARIELGDTVTVVGRVLPFADLDD
                     PSGANLVDGSGIDSADPEIAADLAEARAAGLLAPTPEEAWGNAAIEGFGIGKPVRTPE
                     LDPAADRPPPPDPTLAARAAAAFEIAPDALILATSEEVPMLISLGAPTQVAARAEGQF
                     VLGLLGAALAIGSAMALAVMVQGGLK"
     gene            complement(2990..3961)
                     /locus_tag="VHM48_06580"
     CDS             complement(2990..3961)
                     /locus_tag="VHM48_06580"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX2755109.1"
                     /translation="MHIDRRLVGFGLFLITVGGIMVAVRQGLLSADAASQAWTLWPLI
                     LVGVGLSIVLAGRPGAAIGGLVLALTFGAMLGGIAATGTFPGVGFCGGDRGHGSTFPG
                     SSGDLGPNARVTVVQDCGDLTIGTVAGTSWNVSGESRDGRQPRIDRGSDTLRITAPDG
                     GPFDLGRSSGWDVILGRESTIDLTVTMNGGQSRVNLAGASVGAFRMDANAGSIELDLR
                     EVAAFGDATIDVNFGSATLRLPNRSMTATLAVNAGSAAICLPAGAGLRVKLDSAAASN
                     DFDDHGMVETNGAWETPGYGVAAVRLDLRADVNAGSLALDPPRQCAG"
     gene            4084..4659
                     /locus_tag="VHM48_06585"
     CDS             4084..4659
                     /locus_tag="VHM48_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755110.1"
                     /translation="MRLSEWRASAPARDAAGPKVAAVVDPVLLALGAGPDPHCWVAWG
                     EEPANRFTVFVPTEPGLIVCFVRVTVAGEGPRATTKLVRWSRVSIGELGVETQAGHRM
                     LSFQLESQVLRGVDAEADRAAAFALSVIAAIDGRPLPPIEETRSGRKPAGRPSTAGTT
                     KAKAGTLPTTGAVKPGGPAKARSKAPSNANR"
     gene            4697..6277
                     /locus_tag="VHM48_06590"
     CDS             4697..6277
                     /locus_tag="VHM48_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2755111.1"
                     /translation="MFDLDGVIVDSEIWWDEARRDFARAHGRAWTIDDRHAVMGANSG
                     QWSATMRERLALDLDAATIEAAVVDAMVERYAREGPPTIDGAVEAVRRIADGRATALA
                     SSSHRRVIDAALAATDLAGVFRAVVSSDDVAHGKPAPDVFLEAARRLGADPASTLVVE
                     DSLNGLRAARAAGMTTVLVPNHSVPPADGAETFADLVIARLRDLDVDALGAGGAEAGA
                     PDPAPVAAAYPPPILTSGGAPIATPAVPSGGPAANHPSVPWGPDGWLAAGPHGTDGVH
                     PFRRTLRAWLSRIASWLIVRAYLRLRIEGRDRLPGTPAIYCFNHLSWADPFLLMAILP
                     MRPRLTFFGPKEEDMSVGGRNRLMTWTGATIPYRPGKDNLIEATRRVHAVLAAGGVIA
                     IAGEGRIQPFESQLRPLNEGAAYFALRERVPLVPIAIHGTSWLAFGRRARVVVGEPLA
                     PAGRPNRENVDALTARCWAALHELVRDEPERARPGPIGRWVSEQFNDWPEGDRAAAEA
                     AARAAADEPPGDARPSRS"
     gene            6355..6720
                     /locus_tag="VHM48_06595"
     CDS             6355..6720
                     /locus_tag="VHM48_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755112.1"
                     /translation="MATTGLSADPSVYRERLAEQSDDQIDAWASELMRDIAIRRGVVK
                     VVADFRQAAGLDESSFERVFAFGGGPPAVIGRDRDGRLIVPAITLFALVPGIRSQVAD
                     GRDRLVEYLVANFGELVYV"
     gene            6818..>7119
                     /locus_tag="VHM48_06600"
     CDS             6818..>7119
                     /locus_tag="VHM48_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2755113.1"
                     /translation="MLAGGGIDLAIRIGLAMTLLQLGIGTVNDIVDAPRDAGHKAGKP
                     IPAGLVPAPAARTAAVACFFSGILLAAWVSIVTGVLALVVIGIGLAYDLRLKGTAWS"
BASE COUNT          981 a         2583 c         2576 g          979 t
ORIGIN      
        1 cggtcgtaga cccggccgac gtcagtcgcc ggtcgccctc gacgcctgcg ttcatcgtgg
       61 cgatcgagat cgtggccgac ccggaccgtc tggccgagct ggaggttgtc gtcctcgggg
      121 cctgacgcct gtcgtcaggg cgggcgcggg accggctgag gcggacgagc ccggaatcga
      181 acgaccggcg cggatcgcgc cggtcggatg gtcgtcgaag tgtcgccggc gtcggccgcg
      241 gccggagggt cagcggggct tgagccggtt tgcggccaga tgggtggtcc actcggaggt
      301 ccggccgtcc ggcgaagagc tcttgccgtg ccagccgcac acgcaggtga tcgtctgggc
      361 ctggtgcttg tactcggcga tcacgtgctt caacggctcg ttgacgggct gggcggcggg
      421 acgcggagga cgcatggttc cgttggacac gaaggtgtga cctcccaggc tggatgcggg
      481 cgaggcaggt cggtccgcga gggccatcga cctgacgagg aagcacgcgg cggttgcgcg
      541 aacaagaatt ctactctgaa ccaggcctcc gcgccgggct cggggacgat cggcgccgtc
      601 gccggtcggc gtcagccggc cgggtgcgcc gtcgcgttcc agatctcgac gacgcgccgg
      661 tccgcctccc agccgagctc cgagaccgtc gccgtccaca gctcgagccg actcgcggtg
      721 acgggcggag cctcgatcca gcgggcggcc aggacccgca ggaagtggcc atgcgagaag
      781 acgaggacgt cgccgccgag cggcagcaga cgagcgatca ctcgatcgac ccgccggccg
      841 agcgcagcga ggctctcgcc gccggggatc ggccggctcc agatcgtcca gccgggatcg
      901 tcacgggcaa tgtcgacgcg ccgcctgcct tcgtagatgc cgtagtccca ttcctggagc
      961 tcgggatcga tctcgacgcc ggtgtcgaag ccggcgagac gcgcggtctc gctcgcgcgc
     1021 gacagcgggc tcgagaagac ccggctgaac gaccgacccg cgaggacggc gcccagccgg
     1081 cgtgcctgct cgcgccccac gtcggtcagc gggatgtccg tccggcccgt gtgctggccc
     1141 gccctcgacc atgcggtctc gccgtgccgg gcgagcacga gtcgggtcgg cattggctcc
     1201 gggcccggcg actcgccgcc cgcccgagcc gtcacgcgcc gggctccgcc tcgccgtcgg
     1261 tcagcgatga gggcggccgg tcagcggccg gatcggcatc gaagaacacg cgcgccgtcc
     1321 agccgaacgg gctcgcgagc aggacgcccg gcagctgcgc gatccgggtg ttgtagcgat
     1381 agacctggtc gttgtagagc tcgcgccggt ccgcgatcat cgattcgagc cgttcgatct
     1441 cgctctgcag ggcgaggacg ttggtggcgg acttgatctc cggatagcgc tcgacggtcg
     1501 cgaagaggga tcggaccgcc cgagtcgttg ccgccgaggt cgccgcctgg tccgggatcg
     1561 gggccgtcgg cgagaacgcc gcgcgcgccg cggtcacctc ggccaggacg tcgcgctcga
     1621 acgtcatcac gccgcggacg gcgtccacga gcgccggcag ctggtcgaac cgctgctgga
     1681 gtgccacctc gatgttcgcc catgccttgt cgatccggcg ttgcagggcg acgacgtcgt
     1741 tgtacgtcgc gacgacgacg aacaggacga tcgcgacgac gaccccgacc gcgaaggccg
     1801 ccgcgaggcc ggtcacttca ggcccccctg gaccatgacc gcgagcgcca tcgccgaccc
     1861 gatcgcaagc gccgccccca gcaacccgag gacgaactgc ccctccgcgc gggccgcgac
     1921 ctgcgtcggc gcgccgagcg agatgagcat cgggacctcc tcggacgtgg ccaggatgag
     1981 cgcgtcggga gcgatctcga aggctgccgc ggcgcgggcg gcgagagtcg gatccggcgg
     2041 cggcgggcgg tcggcggcgg ggtcgagctc cggtgtccgg accggcttgc cgattccgaa
     2101 cccctcgatc gcggcgtttc cccacgcctc ctccggggtc ggcgcgagga ggccggcggc
     2161 gcgcgcctcg gccaggtcgg cggcgatctc cgggtcggcg gagtcgatcc cgctgccgtc
     2221 gacgaggttc gcgcccgacg ggtcgtcgag atcggcgaag ggcagcaccc gcccgacgac
     2281 cgtgaccgtg tcgccaagct cgatccgcgc ctcgcgatag tgggcctggc tgcccgtcgc
     2341 cgcgagcggc agcccggcgc ggtcgaggag cgaccagttc agcggggcct tgaccgtcag
     2401 gagtgccgcg atctgagcct cgcgatcctc ggggcccggc gcgtatgccg gcccggtgcg
     2461 cggctggaga ccgaccggac cgccgctcca tgacgaggtg gattcgtcgt agcgatcggg
     2521 gacgtcgaag cgcgcgccat tcggaaagat ccggacggcg ccgctcgcgt cgcggacccg
     2581 gaagccgacc gcccgatcct ccttgaacag gtcgcgctgg ttgtcgtcac gcgtttcggt
     2641 gatccgcgac cggtagtaga cgcaggggat gctctggagc ggcgagatca gggtcagctc
     2701 gatcgcctcg gctgtgccgc tcacgaggac ctcgccgacc gccaggctgg cgatccgcga
     2761 caccgacgtg ccgctgatcc ggccggccgc gcgatacccg ccgaagccac ggatgaggac
     2821 gaccaggccc gcgacgacgc caacggcggc gagtccggcg aagaccgtcc cgggcggcaa
     2881 tgggacgtgg atcatcgcgg gcgtcgacgg agcggacgca gcccgcggac acgcgaatcc
     2941 cagaaaccgc gaacgctcgc gcccccgggc cgaggcaccg gcaaccccgt cagcccgcgc
     3001 actgccgcgg cggatcgagg gcgaggctgc cggcattgac gtcggcccgg aggtccagcc
     3061 ggacggcggc gaccccgtag ccgggcgtct cccacgcgcc gttcgtctcg accatcccgt
     3121 ggtcgtcgaa gtcgttcgag gcagcggcgc tgtcgagctt gacccgcaga ccggcccccg
     3181 ccgggagaca gatcgcggcc gagccggcgt tcaccgcgag cgtggccgtc atgctcctgt
     3241 tcggcaggcg gagcgtggct gacccgaagt tgacatcgat cgtcgcgtcg ccgaacgcgg
     3301 cgacctcgcg gaggtcgagc tcgatcgagc ctgcgttggc atccatccgg aacgccccga
     3361 cactggctcc ggcgaggttg acccggctct gaccgccatt catcgtgacc gtcaggtcga
     3421 tcgtcgactc gcgccccagg atcacatccc agccgctgga ccggccgagg tcgaagggtc
     3481 ctccgtcggg tgccgtgatc cgcagcgtgt ccgagccgcg atcgatccgc ggttggcggc
     3541 cgtctcgcga ctcacccgag acgttccagc tcgttccggc gacggtgccg atggtcaggt
     3601 caccgcaatc ctggacgacg gtcacccggg cattcggccc gaggtcgccg ctgctgccag
     3661 ggaatgtgct gccatggccg cggtcgccgc cgcagaaccc gacgcccggg aacgtgccag
     3721 tggcagcgat gccaccgagc atcgcgccga acgtcagcgc gaggacgagg ccgccgatcg
     3781 cggccccggg tcggccggcg aggacgatcg agagcccgac gccgacgagg atcaacggcc
     3841 acagcgtcca tgcctggctg gcggcgtccg cgctgagcag gccctggcgg accgcgacca
     3901 tgatcccgcc gaccgtgatc aggaacaggc cgaaaccgac caaccgccga tcgatatgca
     3961 tcccgtccaa tcctccagcc ggttcgcgac ggcaacggta caggactgtg ctagcctcgg
     4021 ccggaccggt ccgcccgcgg gatcgccgcg gatcgcggct ggcagcccga cccggaggtg
     4081 gtgatgcgac tcagcgaatg gcgggcctcg gccccggcca gggacgccgc cggacccaag
     4141 gtcgctgccg tcgtcgaccc ggtcctgctg gccctcgggg ccggtccgga tccccattgc
     4201 tgggtcgcct ggggcgagga gcccgccaac cggttcacgg tcttcgtccc gaccgaaccg
     4261 ggcctcatcg tctgcttcgt ccgggtcacc gtcgcggggg agggcccgcg ggccacgacg
     4321 aagctcgtcc gctggagccg ggtgtcgatc ggcgagctcg gcgtggagac ccaggccggc
     4381 caccggatgc tgagcttcca gctcgagtcc caggtactcc gcggggtcga tgccgaggcc
     4441 gatcgggctg ccgcattcgc cctgagcgtg atcgccgcca tcgacggccg gccgctgcca
     4501 ccgatcgaag agacccgctc ggggcggaag ccggcgggcc ggccgagcac cgccggcacg
     4561 acgaaggcaa aggccggcac cctgccgacc acgggcgccg tcaagcccgg cggtccggcg
     4621 aaggcccgtt cgaaggcgcc ctccaacgcc aaccgctgac ccgtcggccg gcccgccggc
     4681 gccgatccgg gcggtcatct tcgatctcga cggtgtcatc gtcgactcgg agatctggtg
     4741 ggatgaggcc cgcagggact tcgcccgggc acacggacgg gcctggacga tcgacgatcg
     4801 gcacgccgtc atgggtgcga actcgggcca gtggtccgca acgatgcgcg agcgtctcgc
     4861 cctcgacctc gatgcagcga cgatcgaggc agccgtcgtc gacgccatgg tcgagcgcta
     4921 cgcgcgcgag ggcccaccga cgatcgacgg ggcggtcgag gccgtccggc ggatcgccga
     4981 tggccgggcg accgcgctcg cgtcctcatc gcaccgccgg gtcatcgacg cggccctcgc
     5041 ggcgacggac ctggccggcg tgttccgtgc cgtcgtctcc tccgacgacg ttgcccacgg
     5101 caagccggcg ccggacgtct tcctcgaggc ggcccgacgg ctcggcgccg atccggcctc
     5161 gacgctggtg gtcgaggatt cgctcaacgg gctgcgggcc gcgcgggcgg cgggcatgac
     5221 gacggtcctc gtcccgaacc actcggtgcc tccggcagac ggtgccgaga cgttcgccga
     5281 cctcgtcatc gcccgactgc gggacctcga tgtcgatgcg ctgggcgccg gcggcgcgga
     5341 ggcgggagcg cctgacccgg cgccggtcgc cgctgcctac ccgccaccga tcctcacctc
     5401 cggcggcgct ccgatcgcga ccccggcagt gccctcgggc ggcccggcgg ccaaccatcc
     5461 gtccgtgccg tggggcccgg acggatggtt ggccgccggg ccccacggca cggacggggt
     5521 ccatccgttc cgacggaccc tccgagcctg gttgtcgcgg atcgcgtcgt ggctcatcgt
     5581 ccgggcctat ctccgcctcc ggatcgaggg ccgggatcgg ctgccaggga cgccggcaat
     5641 ctactgtttc aaccatctca gctgggccga cccgttcctc ctcatggcca tcctgccgat
     5701 gcgcccgcgg ctgacgttct tcggcccgaa ggaggaggac atgtcggtcg gtggccggaa
     5761 ccggctgatg acctggaccg gggcgacgat cccgtatcgg ccaggcaagg acaacctgat
     5821 cgaggcgacg cggcgggttc acgcagtcct cgcggccggc ggggtcatcg cgatcgcggg
     5881 ggagggaagg atccagccgt tcgagtcgca gctccggccg ctgaacgagg gcgccgcgta
     5941 cttcgcgctg cgtgagcgcg tgccactcgt accgatcgcg atccacggca cgtcctggct
     6001 ggccttcggg cggcgggccc gcgtcgtcgt cggcgagccg ctcgcgccgg ccggccggcc
     6061 gaaccgcgag aacgtcgacg cattgacggc ccgttgctgg gcagccctcc acgagctcgt
     6121 ccgcgacgag ccggagcgtg cccgtcccgg cccgatcggg cgctgggtct ccgagcagtt
     6181 caacgactgg cccgagggcg accgggccgc tgccgaggcc gccgcccgcg ccgcggcgga
     6241 cgaacccccc ggcgacgccc ggcccagccg gtcctgaccg gtccctgacc ggtccctgac
     6301 ccacgacgac tctggcatac ttgctgcggc cgatccccga accaggagta cgacgtggcg
     6361 acgaccgggc tttcggccga ccccagcgtg tatcgcgagc ggctcgccga gcagagcgac
     6421 gatcagatcg acgcctgggc gtcggagctg atgcgcgaca tcgcgatccg ccgcggcgtg
     6481 gtcaaggtcg tggccgactt ccgccaggcg gctgggctcg atgagagcag tttcgaacgg
     6541 gtgttcgctt tcggcggtgg tccaccggcg gtcatcggcc gcgaccgcga tgggcgcctg
     6601 atcgtgccgg cgatcactct cttcgcgctg gtgcccggta tccgctcgca ggtcgccgac
     6661 ggacgcgacc ggctcgtcga atacctcgtc gcgaacttcg gcgagctcgt ctacgtctga
     6721 ccccgagccc gccgtcgcga cgaggctgct cggctggatc cggctggccc accccttccc
     6781 gtcggtcctc gacggcctcg tcagcggcac gatcgccgtg cttgccggcg gcgggatcga
     6841 tctcgcgatc cggatcggcc tggcgatgac cctgctccag ctcgggatcg ggaccgtcaa
     6901 cgacatcgtc gacgcgccgc gcgacgccgg ccacaaggcg ggcaagccga tcccggccgg
     6961 gctcgtcccg gcgccggccg cccggacggc agcggtcgcc tgcttcttct ccgggatcct
     7021 ccttgcggcg tgggtctcga tcgtcaccgg cgtcctcgcc ctggtcgtga tcgggatcgg
     7081 tcttgcctac gacctgcgcc tgaagggaac agcctggtc
//
LOCUS       DATAIW010000207        11859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183673, whole genome shotgun sequence.
ACCESSION   DATAIW010000207 DATAIW010000000
VERSION     DATAIW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183673"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VHM48_06605"
     CDS             complement(<1..1460)
                     /locus_tag="VHM48_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2755114.1"
                     /translation="MTIRVRPVDRGRSSRDPNRRTLYTNIAFAITVVVAILILVVVGV
                     TTWYSAHLAAAATVGGQTITKDQFVERATVEVFRLQQLASRVTAEVLAGHLTEAEGQS
                     RIDAINNERDDSKGAFSSTIIEKLIDTSLQARFAAELGVVVTPEQVDQRIVDDKTRKE
                     QRHVWVIAVAPALNSGTTDPTDAQKAAARKTADDTLAAIKGGKTFEEEAKAVSTDATK
                     VAGGDLGWLDSTSSEDPDWQTAVFKLDNNGLTDVILGADGTYRIGRVSEIAPAVVDAV
                     WDQKLADAKISQAAYRAAITSEVMRTVLGDKIVADASASGPQKQVQELYIKAPSTTLA
                     DGAIKVRHILYSPKGDPSGASTVPDTDPSWAAAQLKAQAAYDKVKADPRQFDIIARAE
                     SDETQDRGDDGTGGKLPYFDPTSPIDGAFAAAIFKEGLKPGDLLPPFKSSFGWHVVQL
                     MYAPPDLDELTKLRTQAMAAGADFGQLVRDYSEGPKA"
     gene            complement(1703..2800)
                     /locus_tag="VHM48_06610"
     CDS             complement(1703..2800)
                     /locus_tag="VHM48_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX2755115.1"
                     /translation="MGRLGRTIVRRVARLDPLAYVPRGRRLSGARPVVGLVGFYGHGN
                     YGDELFLEVFREHLGADFTLRSLLDPATGSSIAERLGAGIRESDAIVIGGGDILIPWS
                     TSSRYWERTYLRRPVFVAGVGVPTWREPVPRVVEGLRSFLTDPNVQFIGTRDPESTAW
                     IEEHLRPVAPVATAPDLVCGLSLPAVERPAGQPIFGVAVRSRKEPDDLSHVRRLCERA
                     AEMGYRVRRIVLATGRVRERDDVATEGLGLPDTELVSSDDLDVISRAIGECSAITSMK
                     FHGVVVATMYGVPAIATMPTTKTRNFMRRIDRLDLLAAYSDPELPSFLRPDLAPIADE
                     TRATLRAGAVAHLVDLRDRILVATNEGHRAG"
     gene            2992..3798
                     /locus_tag="VHM48_06615"
     CDS             2992..3798
                     /locus_tag="VHM48_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755116.1"
                     /translation="MTLVRLGIGELLSRRRLIRYLVQADLKKKGADTLLGNIWWVIDP
                     LLQMLIYVVLVSVIFDKGEKDYPLFIFCAILPWKWFTSSVNEGISSVVGMERLIKQIN
                     FPKLVLPVAGVMAGVANFFFGLIPLGVLIALFYPSHATTYLVLIPVVAFVQLLFTLPI
                     AIVLSAINVFYRDVGNLSRHVLRLAFYVSPGIYSILLIQKAAEKYPVLGPFILGNPWT
                     ILFTSYRDVIYEGRAPDWAGLGALSLISLVMLALAILIFKRLEPTFAKVL"
     gene            3800..4657
                     /locus_tag="VHM48_06620"
     CDS             3800..4657
                     /locus_tag="VHM48_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755117.1"
                     /translation="MTNEHGDSPASKVAINVEGLGIRYNLKLTRKATVRQSLTRRVKF
                     EGTDSDFWALRDVTFKVVHGESLGVIGPNGAGKSTLLQVLAGIITPSAGVVEVDGHIS
                     SLLSLGAGFDGDLSGRDNIRLAGAFMGLEHSVVEKRLPGMVEFADLGQFIDAPIKTYS
                     SGMKARLGFAIATSVEPDILLLDEVLSTGDAAFRDKSKARVLELVTEAKAIVLVTHDM
                     GWVSEYCNKAMLLEKGRIVAVGSPDDVVALHREHSEERKAARVAAGVMPLPPPSIPPA
                     SPAATSARP"
     gene            4760..6421
                     /locus_tag="VHM48_06625"
     CDS             4760..6421
                     /locus_tag="VHM48_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2755118.1"
                     /translation="MSFALAGYALLRGSRPRGDGLSTYHEPRINRVAFNRAESALDAG
                     DLETALERARELAHAHPDSIRVLRLRRDIESKQGELSAQARTLHRIQVLDPSPETLRA
                     ERMLLGRIVETSAGWLPRIPGPARPVVPHDGVVLNLLKESLPYLTNGFTMRSRYNLLA
                     VRDAGMQPHVVTNLGFPRLLGGSVASPVPAVEMVDGVPHHRLDLGPNHLPGDRPFDVV
                     LEEQAWLTAAIARQIAPSIIHAGSGHRGYETALVGAALRAHIRRPLVYEVRSFFESTW
                     SADAQWNERGEQYHRRHNAETAAMRGADHVVTIAEAMRDDIIARGVDPDRVTVIPNGV
                     DAEAFTPGPPDPALRRRYGLEGRFTFGYVSNLDHPRENQELLVEATAILLKRGRAVTC
                     LIIGDGKRRAEIEGIAKKAGVGDRVIFTGRVPHEAVPSHYALLDAFVVPRRDERAART
                     VTPLKPYEALAMERPLVVADLPALTEIAEPDARGLAFPAGDAAALATALERLIDDPAL
                     GRRMGVAGREWVARERSWIANGPRFRTVYDQVLERWDRTSSGDGG"
     gene            6421..7602
                     /locus_tag="VHM48_06630"
     CDS             6421..7602
                     /locus_tag="VHM48_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2755119.1"
                     /translation="MDGQLHSLSQAPGEFTDEAIAALRLLSAGEREPLLLAYHPIARM
                     NPFHALLYQRSWQSGMAAVRILRDETFPELTEVARLGFSTVLHLHWLNLVLAQSGSAK
                     EARKDRVAFLARLDRYRDVGGRLVWTVHNVLPHGMRFEDEEAQLSADIAERCDVIHTM
                     AAATPEIVAPWFRIPPEKILQVPHPSYIGAYEDRISREQARHELGLSPDENVHVVLGA
                     IRPYKGLTELLDAWDAIAGDGTPRRLVIAGGPSEETGVAELLDRAAAHPSVLLHAGPV
                     APADVQIYLRAADAAVLPYVRSLNSGALLLALTFGLPAVVPSGGGLAEVVDERFARSF
                     AIEDPASLVAALRDIETLEPLAARAAALEVAASHAPAVVSAQFVEGLRARLAAPASET
                     S"
     gene            complement(7676..8890)
                     /locus_tag="VHM48_06635"
     CDS             complement(7676..8890)
                     /locus_tag="VHM48_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2755120.1"
                     /translation="MSSSEAPAAPSGGSGAAAALDRIPLAEPTIGGNAAAYLQACLDT
                     NYVSSVGPFVERFETEFAAAVGSRFAVACASGTAAIHLALRVLGVGPGDEVFVPTLTF
                     VASANPVAYVGATPVFVDSEEATFNLDPALVVGELDRRARLGLRQPAAIEVVDLVGHP
                     AAIEPIVEAAEWHGVTVFEDASEALGATYVGGTYGGRHVGTIGRVGCFSFNGNKLITT
                     GGGGMLVTDDEALARHARSLSTQARLPGRAYDHAEVGYNYRLTNVAAALGVAQLEQLP
                     ELLAARRANAAHYDNAIADLPGLRPAPRAPWADPSFWLYTASLDPATAGIERDAVLTG
                     MAEAGIEARPIWTPLHLTKLYADAPRLGGSVAERIFANAFSLPSSSSLSDASRDRVVA
                     RLRELLGTSPAG"
     gene            complement(8877..9878)
                     /locus_tag="VHM48_06640"
     CDS             complement(8877..9878)
                     /locus_tag="VHM48_06640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2755121.1"
                     /translation="MAELSLRDTPVLVTGADGFIGSHLVERLVAEGARVRAFCIYNSR
                     GSAGWLDELDPATRAAIDIRLGDIRDARFVERATEGIEVVFHLAALIAIPYSYTAVES
                     FIDTNVRGTMHVLEAAGRAGVRRIIQTSTSEVYGTPDTLPIRETHPLRAQSPYAASKV
                     AADQLALAYHHSFGAPVVVLRPFNTYGPRQSERAVLPTMLRQLLAGRTEIRLGRLDPR
                     RDLTFVVDTVDGFVRAATAAAIEGDTIQLGTGRADSIGELFEMACRVLGVEARAVVDP
                     ARLRPDASEVLVLRSDPTHAREVLGWEAGTSLEDGLAATIDWLRTQPGLAEVDRVQL"
     gene            complement(9871..10917)
                     /locus_tag="VHM48_06645"
     CDS             complement(9871..10917)
                     /locus_tag="VHM48_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2755122.1"
                     /translation="MTDALAACLVPETGTIREAMVALDRGAGRIALAVAGDGRLTGVV
                     TDGDIRRALLGGASLDAPVGPILTRSYLLVAPGDGRADVLELMRARRISAIPVVDGAG
                     FPVGLHLLHEFLERVSRTNWAVVMAGGQGTRLRPLTDTIPKPMLRVAGRPILERIVLH
                     IVGIGIERIFVSVNYLGDVIESHFGDGSAFGARIEYLREDRPLGTAGALGLLPEPPTE
                     PLLLLNGDLVTSADLGGLLAFHDAGRFDSTIGFRRYLHTIPFGAIERDGDRVVGLEEK
                     PTVEREVNTGMYALNPTVVGLVRAGEPTSMPDLIATALDAGRSVGAFEIEDDWVDVGQ
                     REQLVRARGGGGGG"
     gene            complement(10910..11578)
                     /locus_tag="VHM48_06650"
     CDS             complement(10910..11578)
                     /locus_tag="VHM48_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEX2755123.1"
                     /translation="MTARSLAIVGGGEHAVVVLEAARSRPGTWNVVGFSDRSRSSRLA
                     TREPGIEDFGDDGVFADRLAGDPDHAPALVLGIGAGTRPGDRAATVSRFEPDAEWATI
                     VHVTASVSPSARLGPGTVVGAAAVVQAGVVAGRHVIVNSGAIVEHDVVLGDFSHVAPG
                     AAIGGGTRIGQGAFVGLGARVRDHVVIGDRATIGMGAVVVGDVGPDRTIVGAPAREID
                     DADD"
     gene            complement(11575..>11859)
                     /locus_tag="VHM48_06655"
     CDS             complement(11575..>11859)
                     /locus_tag="VHM48_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX2755124.1"
                     /translation="DHVRLAAPAVDLPAVDVGDRQAGRRRETNVIEAPLEAERVAAAL
                     RRALDPTFRAGLANARPSSLDGRAGERIAAIIGAWRPQIPPRKPPINLDP"
BASE COUNT         1694 a         4218 c         4212 g         1735 t
ORIGIN      
        1 gccttcgggc cctccgagta gtcacggacc agctggccga agtccgcgcc ggcggccatg
       61 gcctgggtcc tgagcttggt cagctcgtcg aggtccggcg gcgcgtacat cagctggacg
      121 acgtgccagc cgaacgagct cttgaacggc gggagcaggt ccccgggctt caggccctcc
      181 ttgaagatcg ccgccgcgaa cgccccgtcg atcgggctcg tcgggtcgaa gtagggcagc
      241 ttgccgcccg tcccgtcgtc gccgcgatcc tgcgtctcgt cgctctcggc ccgggcgatg
      301 atgtcgaact gcctggggtc ggccttgacc ttgtcgtagg ccgcctgcgc cttgagctgg
      361 gccgctgccc aggacgggtc ggtatcgggg accgtcgagg cgcccgacgg atcgcccttc
      421 ggcgagtaca ggatgtggcg gaccttgatc gcaccgtcgg cgagagtcgt gctcggggcc
      481 ttgatgtaca gctcctggac ctgcttctgg ggaccggagg cgctggcgtc ggcgacgatc
      541 ttgtcgccga ggaccgtccg catgacctcg ctcgtgatcg cggcgcgata ggcggcctgg
      601 ctgatcttgg cgtcggcgag cttctggtcc cacaccgcgt cgaccacggc cggcgcgatc
      661 tcgctgaccc gcccgatccg gtacgtgccg tcggcaccga ggatgacgtc ggtcaggccg
      721 ttgttgtcga gcttgaagac ggcggtctgc cagtcgggat cctccgagga tgtgctgtcg
      781 agccagccga ggtcgccgcc ggccaccttc gtcgcgtcgg tcgagaccgc cttggcctcc
      841 tcctcgaacg tcttgccgcc cttgatcgcg gcaagggtgt cgtcggccgt cttcctggcc
      901 gcggccttct gggcgtcggt cgggtcggtc gtgccgctgt tgagcgcggg cgcgacggcg
      961 atcacccaga cgtggcgctg ctccttgcgc gtcttgtcgt cgacgattcg ctggtcgacc
     1021 tgctcgggcg tgaccacgac gccgagctcc gcggcgaacc tggcctggag cgacgtgtcg
     1081 atcagcttct cgatgatcgt cgagctgaag gcgcccttcg aatcgtcgcg ctcgttgttg
     1141 atggcgtcga tccggctctg gccctcggct tcggtcaggt gcccggccaa gacctccgcg
     1201 gtgacccggc tcgcgagctg ctggaggcgg aagacctcga ccgtcgcccg ctcgacgaac
     1261 tggtccttcg tgatcgtctg gccaccgacc gtcgcggcag ccgcgagatg ggcgctgtac
     1321 caggtggtca ccccgaccac gacgaggatg aggatcgcga cgaccacggt gatcgcgaac
     1381 gcgatgttcg tgtagagcgt gcggcggttc gggtctcggc tcgaacggcc gcggtcgaca
     1441 ggcctgactc ggatggtcat ggaggattgc ggtcctgatc gtgaaggatg gacggcggtg
     1501 tggccaccgg acgggccgac atcgtaccgt acccggcctg cccgcctcgt gggcgcgcgg
     1561 gttggtggcc cactcgcggg cggcggcgcg cctcatcgcg ggcggcggcg cgcctcgcgg
     1621 ctcgcgtagc cagttcggat agccgaatat cccgggatcg tcgcggacct gcgctgggtt
     1681 gcgttcggag gacgccgggc gatcagccgg cgcggtggcc ctcgttcgtg gcgaccagga
     1741 tccgatcccg caagtccacg aggtgggcga cggcgccggc ccgcaaggtg gcccgtgtct
     1801 cgtccgcgat cggggcaagg tccggccgga ggaacgacgg gagctcgggg tcgctgtatg
     1861 cggccaggag gtcaagccgg tcgatcctgc gcatgaagtt gcgggtcttg gtggtcggca
     1921 tcgtggcgat cgccggaacg ccgtacatcg tcgcaacgac gacgccgtgg aatttcatgc
     1981 tcgtgattgc cgagcactcg ccgatcgcgc ggctgatgac gtcgaggtcg tcggacgaga
     2041 cgagctccgt atcgggcagc ccgaggccct ccgtggcgac gtcgtcgcgc tcgcggacgc
     2101 ggccggtggc gaggacgatc cggcgaacgc ggtagcccat ctcggcggcc cgctcgcaca
     2161 gtcgccggac atggctgagg tcatcgggct ccttccggct ccggacggcg acaccgaaga
     2221 tcggctggcc cgccggccgc tcgacggctg ggaggctgag cccgcagacg agatccgggg
     2281 ctgtcgcgac cggagcgacc ggacgcaggt gctcctcgat ccacgcggtg ctctcggggt
     2341 cgcgcgtccc gatgaactgg acattggggt cggtcaggaa cgagcgcagc ccttcgacga
     2401 cgcggggcac cggctcgcgc caggtcggga cgccgacccc ggccacgaag accggtcggc
     2461 gcaggtaggt ccgctcccag tagcgcgagg aggtcgacca cgggatcagg atgtcccctc
     2521 caccgatgac gatcgcgtcc gactcgcgga tgccggcgcc cagacgctcg gcgatggagc
     2581 tcccggttgc cgggtcgagg agcgagcgaa gcgtgaagtc ggctccaagg tgctcccgga
     2641 acacctcgag gaagagctcg tcgccatagt tcccgtggcc gtagaagcca accaggccga
     2701 cgaccggacg cgcgcccgac aggcgccggc cgcggggaac gtaggcgagc ggatcgagac
     2761 gggcgacgcg ccggacgatg gtccgaccga ggcggcccat aaagcgcacg ggggctggct
     2821 tcatggtcga atggtgccat cccggcgagc gcggcgcggc cgatctcgca cggtcctgcc
     2881 gacggacggg tcgaccggcc cggcggagac tgcatgctat tctcggccga tatggcagtc
     2941 gcttccgagg caccggcgtc ctttcggtcg tcggcgatcc gtccaggacc gttgaccctc
     3001 gtccggctag gaatcggcga actgctctcg cgcaggcgcc tgatccgcta cctcgtccag
     3061 gcggatctga agaagaaggg cgccgacacc ctcctgggca acatctggtg ggtcatcgac
     3121 ccgctcctcc agatgctcat ctacgtcgtc ctcgtgtcgg tcatcttcga caagggcgag
     3181 aaggactacc cgctcttcat cttctgcgcg atcctgccct ggaagtggtt cacctcgtcg
     3241 gtcaacgagg ggatctcgtc ggtcgtcggg atggagcggc tgatcaagca gatcaacttc
     3301 cccaagctcg tcctgcccgt cgcgggggtc atggccgggg tcgcgaactt cttcttcggg
     3361 ctgatcccgc tcggcgtcct gatcgccctg ttctacccga gccacgccac gacctacctg
     3421 gtcctgatcc cggtcgtcgc cttcgtccag ctcctgttca cgttgccgat cgcgatcgtg
     3481 ctcagcgcga tcaacgtctt ctaccgcgac gttggcaacc tgtcccggca cgtcctccgg
     3541 ctcgccttct acgtctcgcc cgggatctac tcgatcctcc tcatccagaa ggccgccgag
     3601 aagtacccgg tcctcggccc gttcatcctg ggcaacccgt ggacgatcct cttcacctcg
     3661 taccgcgacg tgatctacga gggcagggcg cccgactggg ccgggctcgg cgccctcagc
     3721 ctgatctcgc tcgtcatgct cgccctggcg atcctgatct tcaagcggct cgagccgacg
     3781 ttcgccaaag tcctgtgaga tgacgaacga gcatggcgat tccccggcct cgaaggtcgc
     3841 gatcaacgtc gagggtctgg ggatccgcta caacctgaaa ctgacccgca aggcgacggt
     3901 ccgccagtcg ctgacccggc gcgtcaagtt cgaggggacc gacagcgact tctgggcgct
     3961 ccgcgacgtg acgttcaagg tcgtccacgg cgagtcgctc ggcgtcatcg ggccgaacgg
     4021 agccggcaag agcaccctcc tccaggtcct ggccgggatc atcacgccgt cggccggggt
     4081 cgtcgaggtc gacggccaca tctcgagcct cctgtcgctc ggcgccggct tcgacggcga
     4141 cctgagtggg cgcgacaaca tccgcctggc cggggcgttc atgggcctcg agcactcggt
     4201 cgtcgagaag cgcctgccgg ggatggtcga gttcgccgac ctcggccagt tcatcgacgc
     4261 cccgatcaag acctactcgt cgggcatgaa ggcccggctc ggcttcgcga tcgcgacctc
     4321 ggtcgagccc gacatcctcc tgctcgacga ggtcctctcg accggcgacg ccgcgttccg
     4381 cgataagtcg aaggcgcgcg tcctcgagct cgtcacggag gcgaaggcga tcgtcctcgt
     4441 cacccacgac atgggctggg tcagcgagta ctgcaacaag gcgatgctcc tcgagaaggg
     4501 ccggatcgtc gcggtcggca gccccgacga cgtcgtcgcg ctccaccgcg agcactccga
     4561 ggagcgcaag gccgcccggg tggccgccgg cgtgatgccg ctccccccgc cgagcattcc
     4621 gcccgcatca ccagcggcaa cgtccgcccg accctgagcc gcggaccgca tgcccatcga
     4681 cgatcggttt gcccggcgcg cccagcgcgt cgtccggcgt ctggcaggtc ggctcgggtc
     4741 gccccgggac gaggccctga tgagcttcgc gctggcgggc tatgcgctgc tccggggtag
     4801 ccggccgcgc ggcgacggcc tgtcgaccta ccacgagccg cggatcaacc gggtcgcatt
     4861 caaccgcgcg gagtcggccc tcgacgcagg cgatctcgag acggcgctgg agcgggcaag
     4921 ggagctcgcc cacgcccacc ccgacagcat ccgcgtcctt cgcctccggc gggatatcga
     4981 gtcgaagcag ggcgagctca gcgcccaggc gcggaccctc caccggatcc aggtcctgga
     5041 cccgagcccc gagacgctcc gggcggaacg gatgctgctc ggccggatcg tcgagacatc
     5101 ggccggctgg ctgccccgga tccccggacc ggcccggccc gtcgtcccgc acgacggcgt
     5161 cgtcctgaac ctcctcaagg agtccctgcc gtacctgaca aacggcttca cgatgcgttc
     5221 gcgctacaac ctgctggctg tccgcgacgc cgggatgcag ccgcacgtgg tcaccaacct
     5281 cggcttcccg cgtctccttg gcggctcggt tgcctcgccg gtgccggcgg tcgagatggt
     5341 cgacggggtt cctcaccacc ggctcgatct cggcccgaac catctgccgg gtgatcgccc
     5401 cttcgacgtt gtcctcgagg agcaggcctg gctgaccgcg gcgatcgcgc ggcagatcgc
     5461 gccgtcgatc atccatgccg gatccggcca ccgtggctac gagacggccc tcgtcggcgc
     5521 cgcgctgcgg gcccacatcc ggcggccgct cgtgtacgag gtccgctcgt tcttcgagtc
     5581 gacctggtcg gccgacgcgc aatggaacga gcgtggcgag cagtaccacc ggcggcacaa
     5641 cgccgagacg gccgcgatgc gcggcgccga ccacgtcgtc acgatcgcgg aagcgatgcg
     5701 cgacgacatc atcgcccgcg gcgtcgaccc cgatcgggtg acggtcatcc ccaacggcgt
     5761 cgacgccgag gccttcaccc ccgggccgcc cgacccagcc ctgcgccgga ggtacgggct
     5821 cgagggccgc ttcacgttcg gctacgtgag caacctcgac catccgcgcg agaaccagga
     5881 gctcctggtc gaggcgaccg cgatcctcct caagcgcggc cgcgcggtca cctgcctgat
     5941 catcggcgac ggcaagcgcc gcgcggagat cgaggggatc gcgaagaagg ccggcgtcgg
     6001 cgatcgcgtc atcttcaccg ggcgggtgcc gcacgaggcc gtcccgagcc attacgccct
     6061 gctcgacgcg ttcgtcgtgc cgcgccgcga cgagcgggcg gctcgcacgg tcacgccgct
     6121 caagccctac gaagcgctcg cgatggaacg gccgctcgtc gtcgccgacc tcccggcact
     6181 gaccgagatc gccgagccgg acgcgcgcgg actggccttt ccggccggcg acgcggccgc
     6241 cctggcgacg gcgctggaac ggctcatcga cgatccggcg ctcggccggc ggatgggcgt
     6301 ggcgggccgc gagtgggtcg cccgcgaacg gagctggatc gccaacggac cccggttccg
     6361 caccgtctac gaccaggtgc tggagcgctg ggaccggacg tcgtccggag acggcggctg
     6421 atggacggcc agcttcacag cctgagccag gcgccgggcg agttcaccga cgaggcgatc
     6481 gccgcgctcc ggttgctgag cgccggcgag cgcgaaccgt tgcttctcgc ctaccacccg
     6541 atcgcccgga tgaacccgtt ccacgcgctt ctgtaccagc ggagctggca atccgggatg
     6601 gccgccgtac gcatccttcg cgacgagaca tttccggagc tcacggaggt cgcccgactc
     6661 gggttctcga cggtcctcca cctccactgg ctcaacctgg tcctggccca gtcgggctcc
     6721 gcgaaggagg cgcgcaagga ccgcgtcgcg ttcctcgccc ggctcgatcg ctaccgcgac
     6781 gtcggtggcc gcctcgtctg gaccgtccac aacgtccttc cccacgggat gcggttcgag
     6841 gacgaggaag cccagctcag cgccgacatc gcggagcgct gcgacgtgat ccacacgatg
     6901 gccgcagcga ccccggagat cgtcgcgccg tggttccgga tcccgccgga gaagatcctc
     6961 caggtcccgc acccgagcta catcggggcg tacgaggacc ggatcagccg cgagcaggct
     7021 cgccacgagc ttggtctctc gccggacgag aacgtccacg tcgtgctcgg cgcgatccgg
     7081 ccctacaagg gcctgacgga gctgctcgac gcgtgggacg cgatcgccgg cgacgggacg
     7141 ccacgccggc tcgtcatcgc cggcggcccg agcgaggaga cgggcgtggc cgagctcctc
     7201 gatcgagcgg cggcccaccc atcggtgctg ctccatgccg ggccggtggc gcccgccgac
     7261 gtccagatct atctccgggc ggccgacgcc gccgtcctgc cgtacgtccg gtcgctgaac
     7321 tcgggcgccc tcctgctcgc gttgacgttc gggctgcccg ccgtcgtgcc gtcgggcggc
     7381 ggcctggccg aggtcgtcga cgagcggttc gcccggtcgt tcgcgatcga agatccggca
     7441 agcctcgttg ccgcgctgcg ggacatcgag acgctcgagc cgctggcggc ccgcgccgcg
     7501 gcgctcgagg tcgcagcgag ccatgccccg gccgtcgtct cggcgcaatt cgtggagggg
     7561 ctgcgggcgc gtctcgcagc acctgcttcg gagacctcct agccccgacg gacgcgtgac
     7621 ggggggcgcc ggttgccgtc tggctacgtt tcagcgcgag tcgggtgacg acgaactagc
     7681 ctgcgggcga cgtcccgagc aactcgcgga ggcgggcgac gactcggtca cgtgaggcgt
     7741 cgctgaggct cgacgaggac ggcagcgaga aggcgttggc gaagatccgt tcggcgacgg
     7801 atccaccgag ccgcggcgca tctgcgtaca gcttggtcag gtggagcggt gtccagatcg
     7861 gccgggcctc gatcccggcc tccgccatgc cggtcagcac cgcatcccgc tcgatcccgg
     7921 cggtcgcggg atcgagcgac gcggtgtaga gccagaacga cgggtcggcc cacggggcgc
     7981 gcggcgcggg ccgcaggccg ggcaggtcgg cgatcgcgtt gtcgtagtgg gcggcgttcg
     8041 cgcgacgggc ggcaaggagc tcgggcagct gctcgagctg ggcgacgccg agcgcggccg
     8101 cgacgttcgt gaggcggtag ttgtagccga cctcggcatg gtcgtaggcc cggccgggca
     8161 ggcgagcctg cgtcgagagg ctccgggcgt gccgggcgag cgcctcgtcg tcggtgacga
     8221 gcatcccgcc gccgccggtc gtgatcagct tgttgccgtt gaagctgaag cagcccaccc
     8281 gcccgatcgt tccgacgtgg cggccgccgt acgtgccgcc gacataggtc gcgccgagcg
     8341 cctccgaggc gtcctcgaag acggtgacgc cgtgccattc ggccgcctcg acgatcggct
     8401 cgatggcggc cggatggccg accagatcga cgacctcgat cgccgccggc tggcgcagac
     8461 cgagccgggc gcgccgatcg agctccccga cgacgagggc cggatcgagg ttgaaggtcg
     8521 cctcctccga atcgacgaag acgggcgtcg caccgacata ggcgacggga ttggccgacg
     8581 cgacgaacgt cagggtcggg acgaagacct cgtcgcccgg cccgacgccg aggacgcgca
     8641 gggcgagatg gatcgccgcg gtcccgctgg cgcaggcgac ggcaaatcgc gagccgaccg
     8701 cggccgcgaa ctcggtctcg aagcgctcga cgaacgggcc gaccgacgag acgtagttcg
     8761 tgtcgaggca cgcctggagg taggcggcgg cgttgccgcc gatcgtcggt tcggcgaggg
     8821 gaatgcggtc gagcgcggct gccgcgcccg atccgcccga cggtgccgcg ggcgcctcag
     8881 agctggacac ggtcgacctc ggccagcccc ggctgggttc gcagccagtc gatcgtcgcc
     8941 gccaggccgt cctcgaggct cgttccggcc tcccagccga gcacctcgcg agcgtgcgtc
     9001 gggtcggatc ggaggacgag gacctcgctc gcgtccggtc ggaggcgggc cgggtccacg
     9061 accgcccgtg cctccacgcc gagcacgcga caggccattt cgaacagctc gccgatcgaa
     9121 tcggcgcggc ccgtgccgag ctggatcgtg tcgccctcga tggccgcggc cgtcgccgcc
     9181 cggacgaacc cgtcgaccgt gtcgacgaca aaggtcaggt cgcgccgcgg atcgagccgg
     9241 ccgaggcgaa tctccgtccg tcccgcgagg agctggcgga gcatcgtcgg caggaccgcc
     9301 cgctccgact ggcgcgggcc gtacgtgttg aacggccgga gcacgacgac cggcgcgccg
     9361 aagctgtggt ggtaggcgag cgcgagctgg tccgccgcaa ccttggacgc ggcgtacggc
     9421 gactgcgccc gaagcgggtg ggtctcccgg atcgggaggg tgtccggggt cccgtagacc
     9481 tcgctcgtcg acgtctggat gatccgccgg acgcccgccc gcccggccgc ttcgaggacg
     9541 tgcatcgtgc cccggacgtt cgtgtcgatg aacgactcga ccgccgtgta cgagtacggg
     9601 atcgcgatca gggcggcgag gtggaagacg acctcgatcc cctcggtcgc gcgctcgacg
     9661 aaccgcgcgt cgcggatgtc accgaggcgg atgtcgatcg cggctcgtgt cgcgggatcg
     9721 agctcgtcga gccagcccgc cgagccacgc gagttgtaga tgcagaaggc gcgaacccgg
     9781 gcgccctcgg ccacgagccg ctcgacgagg tggctgccga tgaagccgtc ggcgccggtg
     9841 accaggaccg gcgtgtcgcg gagcgagagc tcagccaccg ccgccaccgc cgcgtgccct
     9901 cacgagctgc tcgcgctggc cgacgtcgac ccagtcgtcc tcgatctcga aggcgccgac
     9961 gctccggccg gcatcaagag cggtcgcgat caggtccggc atcgaggtcg gctcgcccgc
    10021 ccggacgagg ccgacgacgg tcgggttcag ggcgtacatc ccggtgttga cctcgcgctc
    10081 gaccgtcggc ttctcctcga gcccgacgac ccggtcgccg tcgcgctcga tcgcgccgaa
    10141 cgggatcgtg tggagataac ggcggaaccc gatcgtcgaa tcgaatcggc cggcgtcgtg
    10201 gaacgcgagc aggccgccga ggtcggccga ggtgaccagg tcgccgttga gcaggagcag
    10261 cggctcggtc ggcggctccg gcaggaggcc gagcgcgccg gcggtcccga gcggccggtc
    10321 ctcgcggagg tactcgatcc gcgccccgaa tgcgctcccg tcgccgaaat ggctctcgat
    10381 gacgtcgccg aggtagttga ccgagacgaa gatccgttcg atcccgatcc cgacgatgtg
    10441 gaggacgatc cgctcgagga tcgggcggcc cgcgacccgc agcatcggct tggggatcgt
    10501 gtcggtcagc ggccgcagcc gggtcccctg gccgccggcc atgacgacgg cccagttggt
    10561 ccggctcact cgctcgagga actcatggag gagatggagc ccgacgggga agccggcgcc
    10621 atcgacgacc ggtatcgcgc tgatccggcg agcccgcatc aactcgagga cgtcggcccg
    10681 gccgtcgccc ggcgcgacca agaggtacga gcgggtcagg atcggtccca ccggcgcgtc
    10741 gagcgaggcg ccgccgagca gtgctcgccg gatgtccccg tcggtcacga cgccggtcag
    10801 ccggccgtcg ccggcgaccg cgagcgcgat ccggccggcc cctcggtcga gcgcgaccat
    10861 cgcctcgcgg atcgtgccgg tctcggggac gaggcaggcg gcgagcgcat cagtcatcgg
    10921 cgtcgtcgat ctcgcgtgcc ggggcgccca cgatcgtccg atccggaccg acgtcgccga
    10981 cgacgacggc ccccataccg atcgtcgcgc ggtcaccgat cacgacatgg tcccgcaccc
    11041 gcgccccgag tcccacgaag gcgccctgcc cgatccgggt cccaccgccg atcgccgcac
    11101 ccggggcgac atgggagaag tcgccgagaa cgacatcgtg ctccacgatc gctccgctgt
    11161 tgacgatcac gtgacgcccg gcgacgaccc cggcctggac gacggcagcc gcgccaacca
    11221 ccgttccggg accgaggcgc gcggaaggcg agaccgaggc cgtgacatgg acgatcgtgg
    11281 cccactcggc gtcaggttcg aaacggctga ccgtcgcggc ccggtcgccg ggacgcgtcc
    11341 cggcgccgat cccgaggacg agggccggcg cgtggtcggg atccccggcc aggcgatccg
    11401 cgaagacgcc gtcgtcgccg aagtcctcga tcccgggctc gcgggtcgcc aggcgtgagg
    11461 aacgcgaccg gtcgctgaac ccgacgacgt tccacgttcc cgggcgactg cgggccgcct
    11521 cgaggacgac gaccgcgtgc tcgccgcccc cgacgatcgc cagcgaccgc gccgtcacgg
    11581 gtcgaggttg atcggcggct tgcgcggggg gatctgcggt cgccatgcgc cgatgatagc
    11641 cgcgatgcgc tcgccggccc gcccatcgag cgagctcgga cgggcgttcg cgagccctgc
    11701 ccggaaggtc ggatcgagcg cccggcgaag ggccgcggcc acccgctcgg cttccagcgg
    11761 cgcctcgatc acgttcgtct cgcgccgccg gccggcctgg cgatcgccga catcgaccgc
    11821 gggcaggtcg acggccggcg ccgcgagacg aacgtgatc
//
LOCUS       DATAIW010000208         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_40963, whole genome shotgun sequence.
ACCESSION   DATAIW010000208 DATAIW010000000
VERSION     DATAIW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40963"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VHM48_06660"
     CDS             <1..404
                     /locus_tag="VHM48_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755125.1"
                     /translation="AALFVIPPALGDGNGRWAIPVAILFAVGHGAALVGVARSRDWGR
                     NLTVFVAELGGGLAILDGVALAILDGVALLVGARPFGPDAAAGPGLVAWTAAVYTLLG
                     IAAGRVPVVARLGPIERRRVVYGPSFAEIVR"
     gene            462..842
                     /locus_tag="VHM48_06665"
     CDS             462..842
                     /locus_tag="VHM48_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX2755126.1"
                     /translation="MVTELTLFDAAGGMPFFEALVGRFYDGVADDPILRPLYPEADLA
                     GARHRLTLFLAQYWGGPQAYDAERGHPRLRMRHAPFAIGPAERDAWLGLMRDAIDAAG
                     AAPDVAERLHIYIDMAAEAMRNRD"
     gene            938..2728
                     /gene="guaA"
                     /locus_tag="VHM48_06670"
     CDS             938..2728
                     /gene="guaA"
                     /locus_tag="VHM48_06670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2755127.1"
                     /translation="MAEAEQDPSIDEAAPAGAQHSPEAQRGKTVHGEAANAGHRVLAP
                     DDAEVEAYLETALNAPAPNRSATDRPDTAEPDSVVILDFGSQFAQLIARRVRELHVYS
                     ELIPHDTPWAEIERRNPKAIILSGGPMSVYDEGAPKPDAAIWSGRIPVLGICYGAQLM
                     ALELGGDVLPTTKREYGPASVTITDGEGLFVGLDRDQPVWMSHGDSITRLPEGFTSTA
                     QTDSTAFAGLQAPSRNLYGIQFHPEVVHTPKGRDVLRNFVVGIAGASPTWTPANFIEQ
                     TVAGIRERVNAQAGATASEGLVICALSGGVDSAVAAALVHRAVGDRLTCIYVDHGLMR
                     KKESELLRVTFERDLGMKLVMVDARKRFLARLAGVEDPEQKRKIIGDEFIRVFEEEAE
                     RIKGDGRIDFLTQGTLYPDVIESATSETKAAQKIKTHHNVGGLPADLSFQLIEPLRYL
                     FKDEVRAVGLEMGLPEAMVLRQPFPGPGLAIRIIGEVTAERLDTLREADWIVIDEIKA
                     AGLYRSLWQSFAILTPVRSVGVMGDGRTYANVVAIRAVTSEDGMTADWAKLPYDVLGK
                     ISSRIVNEVPGVNRVVYDISSKPPATIEWE"
     gene            2901..3368
                     /locus_tag="VHM48_06675"
     CDS             2901..3368
                     /locus_tag="VHM48_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755128.1"
                     /translation="MTGGSVPAPRCNWCSALLPSDHEEVCPSCGATLIGDGDTSVPGL
                     TAIDAEAILRGARAAKTKPRGGLLGWISGDSDDGGEASASPGSLAPPPAEVRREMLRL
                     ELEAQVANAQAEVESMAADDAVEHGRSLATEAADSTEGDDATPAGAAEETPPA"
     gene            3390..3794
                     /locus_tag="VHM48_06680"
     CDS             3390..3794
                     /locus_tag="VHM48_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755129.1"
                     /translation="MRGVVSLLGLVLAVIACGPSTSPSTSTSADATALPNGVTRVWPS
                     SGPAVPDVAASLTLNTHCGLENAVIDFGGTLWDVVGYPGRFPNPPLGIGDPVDKGSVT
                     LVLARNAVFQSASGLRLDLVPHSGPKDVFVCY"
BASE COUNT          587 a         1446 c         1401 g          593 t
ORIGIN      
        1 cggccgccct gttcgtcatc cctccggccc tcggcgacgg caacggtcgc tgggcgatcc
       61 cggtcgcgat cctgttcgcc gtcggccatg gggcggcgct cgtcggggtt gcccggagcc
      121 gcgactgggg ccgcaacctc acggtgttcg tggccgagct cggcggcggc ctcgcgatcc
      181 tcgacggcgt cgccctcgcg atcctcgacg gcgtcgccct cctggtcggt gcccggccgt
      241 tcggtcctga tgcggcggcc ggccccggcc tcgtcgcctg gacggccgcg gtctacacgc
      301 tgctcggcat cgccgcgggg cgggtcccgg tggtcgcccg actcggcccg atcgagcgcc
      361 gccgggtcgt gtacggcccg agcttcgccg agatcgtccg ctagcacgac agcttgctgg
      421 agcgccgggt cgcgggcaac cgagcctcct agaatctccg ggtggtcacc gagctcacgc
      481 tgttcgacgc tgccggcggg atgccgtttt tcgaggcgct cgtcggccgg ttctacgacg
      541 gggtggcgga cgatccgatc ctgcgcccgc tgtacccgga ggccgacctg gccggcgctc
      601 gtcaccgcct gacgctcttc ctcgcccagt actggggcgg gccacaggcc tatgacgcgg
      661 agcgtggcca tccccggctc cggatgcgcc acgcgccgtt cgcgatcggg ccggccgagc
      721 gcgatgcctg gctcggcctg atgcgcgacg cgatcgacgc agcgggcgcc gcgcccgacg
      781 tggcagaacg gctccacatc tacatcgaca tggccgccga ggcgatgcgc aaccgcgact
      841 gacgaagttc gacgctggtc ggaccgtggc cgtcacgccg ggtagactgc gcggaacggg
      901 cggatcggtc ggcggagggt gaccattggc ggacggcgtg gccgaggcgg agcaggatcc
      961 atcgatcgat gaggcggcgc cggcgggcgc gcagcattca ccagaggctc aacgcggcaa
     1021 gaccgtccac ggcgaggcgg cgaacgccgg ccatcgcgtc ctggcccccg atgacgccga
     1081 ggtcgaggcc tatctcgaga ccgccctgaa cgctcccgcg ccgaatcgct cggcgacgga
     1141 tcggcccgac accgccgagc ccgattccgt cgtcatcctc gacttcggga gccagttcgc
     1201 ccagctgatc gcgcgtcgcg tccgcgagct ccacgtctac tcggagctga tcccgcacga
     1261 cacgccgtgg gccgagatcg agcggcgcaa ccccaaggcg atcatcctct cgggcggccc
     1321 gatgtcggtc tacgacgagg gcgccccgaa gccggatgcc gcgatctggt cggggcggat
     1381 cccggtcctc ggcatctgct acggcgccca actgatggcg ctcgagctgg gcggtgacgt
     1441 cctcccgacc accaagcgcg aatacggacc ggcctcggtc acgatcaccg atggcgaggg
     1501 cctgttcgtc ggcctcgacc gcgaccagcc ggtctggatg agccacggcg actcgatcac
     1561 ccggctgccg gagggcttca cctcgaccgc ccagaccgac tcgaccgcct tcgccggcct
     1621 ccaggcgccg tcccgcaacc tctacggcat ccagttccac cccgaggtcg tccacacccc
     1681 gaagggtcgc gacgtcctgc gcaacttcgt cgtcgggatc gccggcgcgt ctccgacctg
     1741 gacgccggcc aacttcatcg agcagacggt cgccgggatc cgcgagcggg tcaacgccca
     1801 ggccggcgcc accgcctccg agggcctcgt catctgcgcc ctctcgggcg gggtggactc
     1861 ggccgtcgcg gccgcgctcg tccaccgcgc cgtgggcgat cgcctgacct gcatctacgt
     1921 cgaccacggt ctgatgcgca agaaggagtc ggaactcctc cgggtcacct tcgagcgcga
     1981 cctcgggatg aagctcgtca tggtcgacgc gaggaagcgg ttcctcgccc ggctggccgg
     2041 cgtcgaggac cccgagcaga agcggaagat catcggcgac gagttcatcc gggtcttcga
     2101 ggaggaggcc gagaggatca agggcgatgg ccggatcgac ttcctgaccc agggcacgct
     2161 ctatcccgac gtcatcgaga gcgccacgtc cgagacgaag gccgcccaga agatcaagac
     2221 ccatcacaac gtcggcgggc tgccggccga cctgagcttc cagctgatcg agccgttgcg
     2281 ctacctgttc aaggacgagg tccgggcggt cgggctcgag atgggactgc ccgaggcgat
     2341 ggtcctccgc cagcccttcc cgggtcccgg gctcgcgatc cggatcatcg gcgaggtcac
     2401 cgccgagcgg ctcgacacgc tccgcgaggc cgactggatc gtcatcgacg agatcaaggc
     2461 ggccgggctg taccggagcc tgtggcagag cttcgcgatc ctgacgccgg tccgttcggt
     2521 cggggtcatg ggcgacggtc ggacctacgc caacgtcgtc gcgatccggg ccgtcacctc
     2581 cgaggacggg atgaccgccg actgggcgaa gctgccgtac gacgtcctcg gcaagatctc
     2641 gagccggatc gtgaacgagg tccccggcgt caaccgggtc gtctacgaca tctcgtccaa
     2701 acctccagcg accattgagt gggaatgatc tctccgaggt ccgaggctga gctccgcgaa
     2761 gccatcggcc cgaggattcg cgtgggaaca ggctcgcggt cgcgttctcc cgataggcac
     2821 gaatcgcgag cgtcctagcc cgatcggggg gttcgcgccg gaacctgagg cgttagagtg
     2881 gcgccatgct ggatgaagcg atgaccgggg ggtccgtgcc cgccccacga tgcaactggt
     2941 gctcggcgct cctgccgtcc gatcacgagg aggtctgccc gtcctgcggg gcgaccctca
     3001 tcggcgacgg tgacacctcc gttccgggcc tgaccgcgat cgacgcggag gcgatcctgc
     3061 gcggcgcccg cgccgcgaag accaagccac gtggcggcct gctcggctgg atcagcggcg
     3121 acagcgacga cggcggcgag gcgagcgcct cgcccggttc gctcgctcca ccgcctgccg
     3181 aggtccgccg cgagatgctc cgcctcgagc tcgaggcgca ggtggccaat gcccaggccg
     3241 aggtcgagtc gatggcggcc gatgatgccg tcgagcacgg ccgatcgctg gcgacggagg
     3301 cggcagactc gactgaaggt gacgacgcca ctcccgccgg cgccgccgaa gagacgccgc
     3361 ccgcctgatc gaacgctcct ggtggcgcga tgagaggggt cgtttcactg ctcgggctgg
     3421 tgctcgcggt gatcgcgtgc ggcccgtcga catcgccgtc aacgtcgaca tcggccgacg
     3481 cgaccgccct gccgaacggc gtcacccgcg tctggccgtc gtctggaccg gcggtccccg
     3541 atgtcgccgc gtcgctgacc ctcaacacgc actgcggcct cgagaacgcc gtgatcgact
     3601 tcggggggac gctctgggac gtggtcgggt atcccggccg gttcccgaat ccgccgcttg
     3661 ggatcggcga ccccgtggac aagggatcgg tcacgctcgt cctggcgcgc aacgccgtct
     3721 tccagtcggc atccggcctg cggctcgacc tcgtgcccca ctccgggccg aaggacgtgt
     3781 tcgtctgcta ctgaccggcg ctcgctgcct cgcccgattg cccatacgcc agccgagatg
     3841 gcgttagcca gaacgatgca cgcggatcga cggacaaggg cgtgaatcgt cgccatccga
     3901 caccggtggc ggcgtatggg gattcccggg cgagcaggac aacgacgcgc ggggttgagc
     3961 cgatccaacg ccgccggggc tggcctatgg gagaaaccgc cgcgatccgg ccccggcggc
     4021 gttggat
//
LOCUS       DATAIW010000209         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_41008, whole genome shotgun sequence.
ACCESSION   DATAIW010000209 DATAIW010000000
VERSION     DATAIW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41008"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHM48_06685"
     CDS             <1..489
                     /locus_tag="VHM48_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2755130.1"
                     /translation="PEWPALFTREEARIRATLGARVSRLEHTGSTSVPGLAAKPIIDI
                     TMTVADVNDEPAWVPDLEAAGYVLVIRESEPDWYDHRVFKGPDTNVNLHTFSAGCVEL
                     ERMVGFRDWLRTHDEDRELYESTKRKLLKREWTFVQNYADAKTEVVDAITARAGLPGP
                     RA"
     gene            707..1810
                     /gene="aroF"
                     /locus_tag="VHM48_06690"
     CDS             707..1810
                     /gene="aroF"
                     /locus_tag="VHM48_06690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2755131.1"
                     /translation="MTREEAHRMFVVMARTAGEAEILGVKSAILGEGLTPYDHVGAER
                     TVIAVIGEIGARKAALMSRLGGLSGVEAVTPISRPFKLTSREFHPENTVIRVLDAVIG
                     DGSLTMMAGPCSVESREQLFETADAVAAAGATILRGGAFKPRTSPYSFQGLGVEALRY
                     LAEARERTGLPVITEVMEPNQVDIVAEYADILQIGTRNMQNYSLLTAVGRVARPVMLK
                     RGYGATVEEWLMAAEYIVSSGNPNVVLCERGIRTFETYTRNTMDLAAVPLVHHLSHLP
                     VIVDPSHATGKRWLVAPLAIGGVAVGADGVMVEVHPDPDAALSDAEQQLTLDQFRAMM
                     AELVPVHEHVRAMYAAPIDAAVAGSAGAGPAKH"
BASE COUNT          288 a          630 c          650 g          255 t
ORIGIN      
        1 ccggagtggc cagctctgtt cacgcgcgag gaggcgcgga tccgggcgac cctcggcgct
       61 cgggtcagcc ggctggagca caccggctcg acgtcggtcc cgggtctcgc cgcgaagccg
      121 atcatcgaca tcacgatgac cgtggccgac gtcaacgacg agccggcctg ggtgccggac
      181 ctggaggcgg cgggctacgt cctcgtgatc cgggagagcg agcccgactg gtatgaccac
      241 cgcgtgttca aggggccgga cacgaacgtc aacctccata cgttctcggc cggctgcgtc
      301 gagctcgagc ggatggtcgg gttccgcgat tggttgcgga cccacgacga ggaccgcgag
      361 ctgtacgagt cgacgaagcg gaagctcctg aagcgcgagt ggacgttcgt ccagaactac
      421 gcggacgcga agaccgaagt cgtcgacgcg atcacggccc gggccggcct gccgggacca
      481 cgcgcgtgac ggccgacggt cgtcgaacgc gcgcgccgcg cctggccgcg ccgtcgccgg
      541 gcgcgacggc gcgttgacac cgcgcccggt gcgccgtacc gtccgctgca cgcagatgaa
      601 cgccaccttc tacgttcgac gcccggcatg accgaccccg gagcctaggc ccgggatcgc
      661 gggtcgtgcc gggtggaagc agaggtcgcg acctctgctt ttttgtttga ccagagagga
      721 agcgcaccgg atgttcgtcg tgatggcccg gaccgcgggc gaggccgaga tcctcggcgt
      781 gaagagcgcc atcctcggcg agggcctcac gccgtacgac cacgtcggcg ccgagcggac
      841 cgtcatcgcc gtgatcggcg agatcggcgc ccggaaagcg gcgctgatga gccgtctcgg
      901 cgggctcagc ggggtcgagg ccgtgactcc gatcagccgg ccgttcaagc tgacctcgcg
      961 cgagttccat cccgagaaca cggtcatccg ggtcctcgat gcggtgatcg gcgacggctc
     1021 gctgacgatg atggccgggc cctgctcggt cgagagccgc gagcagctgt tcgagactgc
     1081 cgatgcggtc gccgcggccg gggcgacgat cctccgcggc ggcgccttca agccacggac
     1141 gagcccgtac agcttccagg ggctcggcgt cgaggccctc cgctatctcg ccgaggcgcg
     1201 cgagcggacc gggctgccgg tgatcaccga ggtcatggag ccgaaccagg tcgacatcgt
     1261 cgcggagtac gccgacatcc tccagatcgg cacccggaac atgcagaact actcgctctt
     1321 gaccgcggtc gggcgggtgg cgcgaccggt gatgctcaag cgcggctacg gcgcgacggt
     1381 cgaggaatgg ctgatggcgg ccgagtacat cgtcagctcc ggcaacccga acgtggtcct
     1441 ctgcgaacgc ggcatccgga ccttcgagac ctacacccgc aacacgatgg acctcgccgc
     1501 agtgccgctc gtccatcacc tcagccacct gccggtgatc gtcgacccga gccacgcgac
     1561 cggcaagcgc tggcttgtcg cgccgctggc gatcggcggc gtggcggtcg gggccgacgg
     1621 ggtcatggtc gaggtccacc cggatccgga cgcggccctg tccgacgccg agcagcagct
     1681 gaccctcgac cagttccggg cgatgatggc cgagctcgtg cccgtccacg agcacgtccg
     1741 ggcgatgtac gcggcgccaa tcgatgctgc cgtggccggc agcgccggcg ccggcccggc
     1801 gaagcattga tccacgatgc gaa
//
LOCUS       DATAIW010000210         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_183744, whole genome shotgun sequence.
ACCESSION   DATAIW010000210 DATAIW010000000
VERSION     DATAIW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183744"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VHM48_06695"
     CDS             complement(<1..610)
                     /locus_tag="VHM48_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2755132.1"
                     /translation="MASDADPGTRAPSATARVDLHCHTSASFDSLVDPAAVIARAVTR
                     GLTHIAITDHDVIDGALEAVAAAPPGIHVLVGCESHTVEGDLVFVFPERPIPKGLAAR
                     EAIIAAWEQGALVGVPHPYDPARRSLLLDPANQPLLALVDWIEGWNGRVGRQADNDHA
                     SAAARRRHLPAVAVSDAHTLLEIGTMFTTMTGDPSSPAGLLAA"
     gene            complement(613..1548)
                     /locus_tag="VHM48_06700"
     CDS             complement(613..1548)
                     /locus_tag="VHM48_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2755133.1"
                     /translation="MPQTDQPTPNAAALDSIEGIIDQFRERDYICDRSLATAVFLGLR
                     LGRPLLLEGEAGVGKTELAKVLAASLGAKLIRLQCYEGLDVNAAVYEWNYPRQMLEIR
                     LLEARGEASTANAHDIFGPDFLIRRPLLQALEASADGEPPVLLIDEIDRADEEFEAYL
                     LEILSDFQVTVPEIGTIKADKPPRVILTSNRTREVHDALKRRCLYHWIDYPTAQKEFE
                     IVIARVPDAPERLAHEVVAFVHRLREADLTKVPGIAETLDWAAALLSLGSRELTGEVV
                     DETLGVVLKYEEDIRRIRGTTARAYLSEAGAAAGG"
BASE COUNT          237 a          635 c          691 g          267 t
ORIGIN      
        1 gtgccgccag gagtccggcg ggactcgatg ggtcgcccgt catcgtcgtg aacatcgtgc
       61 cgatctcgag cagcgtgtgg gcgtcggaca ccgcgaccgc cgggaggtgc cggcgacggg
      121 ccgccgcaga tgcgtggtcg ttgtccgcct gccggccgac gcgcccgttc cagccctcga
      181 tccagtctac gagcgcgagc agcggctggt tcgccggatc cagcaggagc gacctgcgtg
      241 ccggatcgta gggatgcggg acgccgacga gcgcgccctg ctcccacgcc gcgatgatcg
      301 cctcgcgggc ggcaaggccc ttcgggatgg gtcgctcggg gaagacgaac accaggtcgc
      361 cctcgacggt atggctctcg caccccacga ggacgtggat cccgggtggg gcggccgcga
      421 ccgcctcgag cgctccgtcg atgacgtcgt gatcggtgat cgcgatgtgg gtcaggcccc
      481 gagtgaccgc ccgggcgatg accgcggccg ggtcgacgag gctgtcgaag ctggcgctcg
      541 tatggcagtg gaggtcgacc cgcgcggtcg ccgacggcgc ccgggtcccc ggatcggcgt
      601 ccgacgccac ggtcaaccgc cggccgccgc tccggcttcg ctcaggtagg cccgggcggt
      661 cgtcccccgg atccggcgga tgtcctcctc gtacttgagg acgacaccga gcgtctcgtc
      721 gacgacctcg ccggtcagct cgcgtgagcc gaggctcagg agcgcggccg cccagtcgag
      781 cgtctcggcg atccccggga ccttggtcag gtcggcctcg cgcagccgat ggacgaaggc
      841 gacgacctcg tgggcgagcc gctcgggcgc atcggggacg cgggcgatga cgatctcgaa
      901 ctccttctgg gccgtcgggt agtcgatcca gtgatagagg cagcgccgct tcagggcgtc
      961 gtggacctcg cgggtccggt tcgaggtcag gatgacccgc ggcggcttgt cggccttgat
     1021 cgtgccgatc tccgggaccg tcacctggaa gtccgacagg atctcgagca ggtacgcctc
     1081 gaactcctcg tcggcccggt cgatctcgtc gatcagcaga accggcggct cgccgtcggc
     1141 gctcgcctcg agggcctgga ggagcggccg gcggatgagg aagtccggcc cgaagatgtc
     1201 gtgggcgttc gccgtcgacg cctcgccacg cgcctcgagc agccggatct cgagcatctg
     1261 gcgcgggtag ttccactcgt agaccgcggc gttgacgtcg aggccctcgt agcactggag
     1321 ccggatcagc ttcgcgccga ggctggccgc caggaccttc gccagctccg tcttgccgac
     1381 gccggcctcg ccctcgagga gcagcggacg gccgagccgg aggccgagga agaccgccgt
     1441 cgcgagggac cggtcgcaga tgtagtcacg ctcgcggaac tggtcgatga tcccctcgat
     1501 cgagtcgagg gcggcggcgt tcggcgtggg ctggtcggtc tggggcatgg cggcgatgct
     1561 acaccgcgac ccggccgtcc gggcgtggcg tcggcgccgt cccgccgagc gccgcgcact
     1621 gcgatcaggc gtttgcgccg cggccgtccc attcgcgcgt cgcgccgccg ttcggtcgcc
     1681 cggtgcccga accgagtgcg gaggcggcgt ggctcagccg tccggtgcgt cgagcccgcg
     1741 cgcgtacggc gggaagcgga agcgcatctc gcgggtcagg accccgccgt ggccgaggtg
     1801 cgacaccgtc cggagggtcg ggggctcgcc
//
LOCUS       DATAIW010000211         8456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_41114, whole genome shotgun sequence.
ACCESSION   DATAIW010000211 DATAIW010000000
VERSION     DATAIW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41114"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..762)
                     /locus_tag="VHM48_06705"
     CDS             complement(154..762)
                     /locus_tag="VHM48_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX2755134.1"
                     /translation="MMFERLFGGREMDPATAERIPPGQYKTDKFPVLHYGSVPRTDLA
                     TWDFKVYGEVDSPFTLSWEQFRALPRKTVHTDIHCVTRWTKLDTEWEGVPIQEILRLA
                     QVRPTATHVVSHAEQGYTANVPLSVLDDDDVLLADTFDGQPLELEHGWPLRLFVPKKY
                     FWKSSKWVRGLEFLDHDQLGFWERYGYHNDADPWKEERFSDG"
     gene            840..1706
                     /locus_tag="VHM48_06710"
     CDS             840..1706
                     /locus_tag="VHM48_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2755135.1"
                     /translation="MADRSITWDRVGFRLVDRPSDVAAGDAGSAPLAGRPKVVIVMPA
                     YNAARTLERTYADIPHDIVDAIILVDDVSRDETVEVARQLGLDVIIHQQNLGYGGNQK
                     TCYEAALMQGADIVVMLHPDYQYDATRIPELIEPIVRGEKHLMLGSRFLGDPLAGGMP
                     RWKYISNRFLTTVENVAFRLRLSEYHTGLRAYSRRLLETIPYGLNSNDFVFDQELIAQ
                     VVAAGMGRRIGEIAVPTRYFEEASSVGFKRSVVYGLSTLRVVARYLLHRIGLRRSAQL
                     TARRPQPPNTTG"
     gene            1724..2701
                     /locus_tag="VHM48_06715"
     CDS             1724..2701
                     /locus_tag="VHM48_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2755136.1"
                     /translation="MRRGPILRALVGGAISIAALWFVLRGTDLARTADLLGRADLRWV
                     ALAAAFMTLDLAFRALRWQRLLRPIASVRYRPMLGYLLIGYLANNILPARLGELVRSH
                     YLGDREGISRAAALGTVVVERVVDLVACVVIASAALLLLSVRGIVANAVLVGAAVTGL
                     LLVVLAIGIVAHRLPGLDRIRSMIDGYPRVRDLARSLQGGLAVAARPRTVGEAILASG
                     ASWTMAVLAFAAIGQAIGIQLSMAQAALIVSGVALASAVPAGPSNLGTFELAAQELGK
                     AVGLEQSSALALGILAHVALLVVTSIGGAIAFARLGWRGGSESTTDDVA"
     gene            complement(2724..3239)
                     /locus_tag="VHM48_06720"
     CDS             complement(2724..3239)
                     /locus_tag="VHM48_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755137.1"
                     /translation="MDLLQDLAPVSESYATLPIPNAFNWQDSTSELGDGEWYLVAFRS
                     IRRADADEQRLTQFDELAHQEAATSPGFVQYFKGPRATDGSCLSFCLWQSRADARAAS
                     SRPDHVRAVSLIDEMYETYALEFHRVARQSGGQLTFEPYDKVPAHGAPAVKLSHRTGA
                     PHLPIQPSPAV"
     gene            complement(3290..4087)
                     /locus_tag="VHM48_06725"
     CDS             complement(3290..4087)
                     /locus_tag="VHM48_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2755138.1"
                     /translation="MTTARDRPPEEAHEDTSFARGLRVLLTIADRGEIRADELSALLE
                     TPISTIYRYLRTLAEFGFIDRQGAGYRLGPRLIIGNGSTVTAEELIRTADPVLHLLAD
                     ETGETAVIVRRIGMSAVCLHEVPSTQPLRVTIAPASALPLQRGAFGKALLAFAPDDVL
                     DEVLGQEPPPGPPGPDPDRLRAELAEVVTSGVARSVGEVIAGTVTIAVPIFREDGIVA
                     AIGVIGPEGRCGLAWRTRVARLLPGAASSVVGALGTGPSSQDSRYPE"
     gene            complement(4071..4457)
                     /locus_tag="VHM48_06730"
     CDS             complement(4071..4457)
                     /locus_tag="VHM48_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX2755139.1"
                     /translation="MPGPDHTAHAGLTYLSLAAQSLTVEARLLAGRVSFKVVSTAHAK
                     GIEDRTTMAPLAARRLAEMVELGGMIVAIERAVRDHVLFLKMSDHVPDVSALAAAARR
                     DALGRAVLDGNGAAGSSRGNGDDDRA"
     gene            complement(4521..5516)
                     /locus_tag="VHM48_06735"
     CDS             complement(4521..5516)
                     /locus_tag="VHM48_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2755140.1"
                     /translation="MRLNRTIALGASLLMVLGACSSGATPSPSASKAASAGVPPSVSS
                     GGASPSVAGATPDIKIGSDDFYESAVVAEMWAQVLEKNGYKVDRHFNIGARQVRQPAL
                     EQGQIDMVPEYVGSGLTYYDKTMTTGDGQQNHDKLQTIVTGKGGGLTVFSISPGQDQN
                     AFVVRADTATQFSLAKISDLVAQQATLTWGLPADCDTNASCSGALKDGYGFTYPPAKR
                     KALNACSGPMAEALQGKSIDIAELCSTQPAILQFGFKLLEDDKKTQGAENLAAMVRND
                     YLAKVGDKAAFQALIDAVTAKLTTEELTKIGVEIQVNHKDVPVEATDWLKANGLL"
     gene            complement(5635..6372)
                     /locus_tag="VHM48_06740"
     CDS             complement(5635..6372)
                     /locus_tag="VHM48_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755141.1"
                     /translation="MSDLIGWFVDPAHFSGTGSIQQRLLEHILLSGASILVATVVGLP
                     IGLYIGHTRRFANLAINIANIGRALPSYALVVMILPVSLTLSPEYGLDPIPTFVAMTL
                     LAIPPILVGAYAGLRSVDADLVEAGRGMGLREGQILGNIELPLASPIIVGGFRTATLQ
                     VIATATIGAIVSGGGLGRFIVDGIHNQDYTTVVGGVILVASLAIGVDLVLALVQRRVT
                     PRGLQLQQARRGQPPDVAMPIPTADPA"
     gene            complement(6372..7034)
                     /locus_tag="VHM48_06745"
     CDS             complement(6372..7034)
                     /locus_tag="VHM48_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755142.1"
                     /translation="MTISIDWGWLSTHVGAMGLRFIQHFQLVAIAVTLGFVISLVLAI
                     WAYRQPRVYPPLTAITGLLYTIPSLAAFAILRPITGLTIVTALIPLTTYTLLILVRAN
                     VAGFNSVPAEVLEAADGMGYTRRQRLLRVELPLALPLMIAGIRLATVTTIGLATVASI
                     LGETFGGFGFFITEGLDRFFLTEIYLGAVLSIVMAFGADLLLVWIERLVTPWARTRAG
                     TA"
     gene            complement(7038..>8456)
                     /locus_tag="VHM48_06750"
     CDS             complement(7038..>8456)
                     /locus_tag="VHM48_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755143.1"
                     /translation="GNGGSSGTLSRSIRMAEILMWEAVSLTIRDAVLSALTRETTSDP
                     PARRIAAPMADQHRQAAVVEFDHVTKRYDVPTGKDKGKPTPGAVNDLSLKVPAGKICI
                     LVGPSGCGKTTSLKMVNRLIEPTAGKIVIDGVDAGTRELTELRRSIGYVIQQVGLFPH
                     QTIGENVATVPRLLGWPKARLKERSEELLGLVGLDPAKYRDRFPSQLSGGERQRVGVA
                     RALAADPPIMLMDEPFGAVDPIVRDRLQNEFLRLQETIAKTILFVTHDIDEAIKMGDL
                     VAVFQTGGILAQFGRPLDILAAPASEFVARFVGQDRGLKRLSLLRVSDVPIRLAVTAR
                     PGDAAADARRRALDDPLRYLLLIDAGNKPIGWVAHGDIPADGTLTEAMANPSSPQLNK
                     RTTLKDALSMMLDADVQTGVVVDRTGAVQGLLSIATIAEKMREGEHAAAFDQVPAAET
                     GEIDEPPPDDQDADPAASAASA"
BASE COUNT         1271 a         2838 c         3017 g         1330 t
ORIGIN      
        1 tggtcccgac agcgacccag ccgagcgggg tcgcggcgac atcgaacagg gttgcacgcc
       61 gccacgaatg cggccacggc cagcgtcacc agccatcgga gccgaacggc cgccccgttc
      121 gccgaatcgg cggcccgcgg ctcgggtcac ggttcagccg tcgctgaagc gctcttcctt
      181 ccacgggtcg gcgtcgttgt ggtagccgta gcgctcccag aaaccgagct ggtcgtggtc
      241 gaggaactcg aggccgcgga cccacttcga gctcttccag aagtacttct tcgggacgaa
      301 gaggcggagg ggccagccat gctcgagctc gagcggctgg ccgtcgaacg tgtcggcgag
      361 gaggacgtcg tcgtcgtcga ggacgctcag cggcacgttc gccgtgtagc cctgctcggc
      421 atgagagacg acatgggtcg cggtcggccg gacctgggcg aggcgaagga tctcctggat
      481 cgggacgccc tcccactcgg tgtcgagctt cgtccagcgg gtgacgcagt ggatgtcggt
      541 gtggacggtc ttgcggggaa gggccctgaa ctgctcccag ctcagggtga agggactgtc
      601 gacctcgccg tagaccttga agtcccaggt cgcgaggtcc gtccgcggca ccgagccgta
      661 atggaggacc gggaacttgt cggtcttgta ctggccgggc gggatgcgtt cggcggtcgc
      721 cgggtccatc tcgcggccgc cgaagaggcg ttcgaacatc atgtctccga gtctgcgggc
      781 aggcgccatc gttgcacatc gggcgccgga gtcgatgtcg ggccgatgac gaccggccca
      841 tggcggaccg ttcgatcacc tgggatcgag taggcttccg cctcgtggac cgcccaagcg
      901 acgtcgccgc cggtgacgcc ggatccgccc cgctcgccgg ccgaccgaag gtcgtgatcg
      961 tcatgcccgc ctacaacgcg gccaggacgc tggagcggac ctacgccgat attccgcacg
     1021 acatcgtgga cgcgatcatc ctcgtcgatg acgtctcgcg ggacgagacc gtcgaggtcg
     1081 cccggcagct cgggctcgac gtcatcatcc accagcagaa cctgggctac ggtggcaacc
     1141 agaagacctg ctacgaggcg gccctcatgc agggcgccga catcgtcgtc atgctccacc
     1201 cggactacca gtacgacgcg acccggatcc cggagctgat cgaaccgatc gtccgcggcg
     1261 agaagcacct gatgctcggc agccggttcc tgggcgatcc cctcgcgggt ggcatgccgc
     1321 gctggaagta catctcgaac cggttcctga cgaccgtcga gaacgtcgcg ttcaggctgc
     1381 gcctgtccga gtaccacacc ggcctgcggg cctacagccg gcgcctgctc gagacgatcc
     1441 cgtacgggct caacagcaac gacttcgtct tcgaccagga gctgatcgcc caggtcgtcg
     1501 cggccgggat gggccgccgg atcggcgaga tcgcggtccc gacccgctac ttcgaggagg
     1561 ccagctcggt cgggttcaag cggagcgtcg tctacgggct gtccacgctc cgcgtcgtcg
     1621 cccgctacct cctccaccgg atcggcttgc gccggagcgc ccagctgacc gcccgccggc
     1681 cgcagccgcc gaacacgacc ggctgagccg gtccgccccc ctgatgcgcc gcggccccat
     1741 tctccgagcg ctggtcggcg gcgcgatctc gatcgcggcc ctctggttcg tcctccgcgg
     1801 caccgatctc gccaggaccg ccgacctcct gggccgagcc gacctccgct gggtcgccct
     1861 tgccgccgcc ttcatgacgc tcgacctggc cttccgggcc ctgcgctggc aacgcctcct
     1921 ccggccgatc gcgagcgttc ggtatcggcc catgctcggc tacctgctca tcggctatct
     1981 cgccaacaac atcctcccgg cccgactcgg tgagctcgtc cgcagccatt acctggggga
     2041 ccgggagggg atcagccgag cggcggccct cgggacggtc gtcgtcgagc gggtcgtcga
     2101 cctcgtggcg tgcgtcgtca tcgcctcggc ggcgctcctg ctgctcagcg tccgcggcat
     2161 cgtcgccaac gcggtcctcg tcggggcagc cgtcaccggc ctgctcctcg tggtgctcgc
     2221 gatcgggatc gtggcccacc ggctgcccgg cctcgaccgg atccgctcca tgatcgatgg
     2281 ctacccgcgg gtccgcgatc tcgcccggag cctccaggga ggactggcgg tcgcggcccg
     2341 gccccgaacc gtcggcgagg cgatcctcgc gagcggcgcc tcatggacga tggccgtcct
     2401 cgccttcgcc gcgatcggcc aggcgatcgg gatccagcta tcgatggccc aggcggccct
     2461 catcgtgtcg ggcgtggccc tcgcgagcgc ggtcccggca ggaccgtcga acctcgggac
     2521 gttcgagctg gccgcacaag agctcggcaa ggcggtcggc ctcgagcaat cgtcagctct
     2581 cgcactcggc atcctcgccc acgtcgccct cctcgtggtg acctcgatcg ggggcgcgat
     2641 cgcgttcgcg aggctcggct ggcgaggtgg atcggagtcc acgaccgacg acgttgcctg
     2701 agcgcgggcg tccggcggaa gcctcagacc gcgggtgagg gctggatcgg caggtgcggc
     2761 gccccggtgc ggtgcgacag cttgacggcc ggcgcgccgt gggcgggcac cttgtcgtac
     2821 ggctcgaagg tcagctggcc gccggactgg cgggcaacgc ggtggaactc gagcgcgtac
     2881 gtctcgtaca tctcgtcgat caagctcacg gctcggacgt gatcgggtcg gctggacgcg
     2941 gcccgggcgt cggcccggct ctgccacagg cagaacgaga ggcacgagcc atcggtcgcc
     3001 cgtggtccct tgaagtactg gacgaagccg ggcgaggtcg ccgcttcctg atgggcgagc
     3061 tcatcgaact gcgtcagccg ctgctcgtcg gcatcggccc gccggatcga gcggaaggcg
     3121 acgaggtacc actcgccgtc accgagctcg gaggtcgaat cctgccagtt gaaggcgttc
     3181 gggatcggca gggtcgcata gctctcggac accggagcga gatcctgcag caggtccatg
     3241 gctgcggagc atagggccgt gcccaggatt gtcaaatact gagatcgggc tattccggat
     3301 atcgggagtc ctgggacgaa ggtcctgtcc cgagcgcgcc gacgacgctg ctcgcggcgc
     3361 cggggagcag gcgcgccacc cttgtccgcc acgcgagccc gcaccgcccc tctgggccga
     3421 tcaccccgat cgcggccacg atcccgtcct cccggaatat gggaaccgcg attgtcacgg
     3481 tgccggcaat cacctctccc accgaccgcg ccacgccgct ggtcacgacc tcggcaagct
     3541 ccgctcgcaa ccggtccggg tccggtccgg gcggtcccgg cggaggttcc tggccgagca
     3601 cctcgtcgag cacatcgtcg ggtgcgaatg cgaggagcgc cttgccgaac gcgcctcgct
     3661 ggagcggcag cgccgatgcc ggtgcgatcg tgacccggag cggttgggtc gaggggacct
     3721 catgcaggca gaccgccgac atgccgatcc gccggacgat cacggccgtc tcgccggttt
     3781 cgtcggcgag gaggtggaga accgggtcgg cggtccggat cagctcctcg gcggtgacgg
     3841 tcgatccgtt gccgatgatc agtcgcgggc cgagccgata gccggcgccc tggcggtcga
     3901 tgaagccgaa ttcggccagg gtccggaggt agcgatagat cgtcgagatc ggggtctcga
     3961 ggagggcgct cagctcatcg gcccggatct cgccgcgatc cgcgatcgtc aggaggacac
     4021 ggagaccgcg ggcgaaggac gtgtcctcat gggcctcctc cggcggtcga tcacgcgcgg
     4081 tcgtcatcgc cattcccccg tgatgacccg gcggccccgt ttccatcgag gactgcccgg
     4141 ccgagagcgt cgcgccgagc ggcggctgcc agcgccgaga cgtccggcac atggtcgctc
     4201 atcttgagga acaggacatg gtcgcggact gcccgctcga tcgcgacgat catgccaccg
     4261 agctcgacca tctcggcgag ccgccgggcg gcgagcggcg ccatcgtcgt gcggtcctcg
     4321 atccctttgg cgtgggcggt cgagaccacc ttgaacgaga cacgcccggc gagcagacgc
     4381 gcttccacag tcagactctg ggcggcgagg ctgaggtacg tcaagcccgc gtgcgcggtg
     4441 tggtccgggc ccggaatcgt cgggaacgaa tgaacccgcc ggcgtggccg gcgggttcac
     4501 gagattggcg gccggggaga ctagagcagg ccgttcgcct tgagccagtc ggtggcctcg
     4561 acgggcacgt ccttgtggtt gacctggatc tcgacgccga tcttggtcaa ttcctcggtc
     4621 gtcagcttgg ccgtgacggc gtcgatcagc gcctggaagg ccgccttgtc gccgaccttg
     4681 gcgagatagt cgttgcggac catcgcggcg aggttctcgg cgccctgggt cttcttgtcg
     4741 tcttcgagga gcttgaatcc gaactggagg atggccggct gggtcgagca cagctcggcg
     4801 atgtcgatcg acttgccttg cagggcctcg gccatcggcc cggagcaggc gttgagcgcc
     4861 ttgcgcttgg cgggcggata cgtgaagccg tagccgtcct tgagcgcgcc cgagcatgag
     4921 gcattggtgt cgcagtccgc cggcagtccc catgtcaggg tcgcctgctg ggccacgagg
     4981 tcgctgatct tcgcgaggct gaactgggtg gccgtgtcgg cacggacgac aaaggcgttc
     5041 tggtcctggc cggggctgat gctgaagacg gtcaggccgc cacccttgcc cgtgacgatc
     5101 gtctgcagct tgtcatggtt ctgctggccg tcgccggtgg tcatcgtctt gtcgtaatac
     5161 gtgagaccgg atccgacgta ctccgggacc atgtcgatct ggccctgctc gagcgcgggc
     5221 tggcgaacct gccgggcgcc gatgttgaag tggcggtcga ccttgtagcc gttcttctcg
     5281 aggacctgcg cccacatctc ggccacgacg gccgattcgt agaaatcatc cgagccgatc
     5341 ttgatgtccg gcgtcgcgcc ggcgacggac ggcgaggctc cgccgctcga aaccgacggt
     5401 ggcacgccgg cggacgcggc cttcgaggca gacggcgacg gtgtcgctcc cgacgagcag
     5461 gcgccgagca ccatcagcag cgacgcccca agggcgatcg tgcggttcaa ccgcatgggt
     5521 ttctcctcct tcattgaacc gttcccgctc cggggccacg gagcggctag ggtagcagca
     5581 tcgtgcgatc gtgcagcccc tcgtggtcgg ggccgcgatg ggccttcgat cgggtcaggc
     5641 cggatcggcc gtcgggatcg gcatggcgac atccggcggc tggccgcgtc gcgcttgctg
     5701 gagctggaga ccacgtggcg tcacgcgccg ctggaccagc gccagcacga gatcgacgcc
     5761 gatcgccagc gaggcgacga ggatgacgcc gccgacgacc gtcgtgtagt cctggttgtg
     5821 gatcccgtcg acgatgaacc ggcccaggcc accgccggac acgatcgccc cgatcgtcgc
     5881 cgtcgcgatc acctggaggg tcgccgtccg gaagccgccg acgatgatcg gcgaggcaag
     5941 cggcagctcg atgttcccga ggatctggcc ctcgcgcagg cccattccgc ggcccgcctc
     6001 cacgaggtcg gcgtcgaccg agcgcagccc cgcgtaggca cccacgagga tcggcgggat
     6061 cgccagcaac gtcatcgcca cgaacgtcgg gatcgggtcg agaccgtact ccgggctcag
     6121 ggtcagggac accggcagga tcatcacgac gagcgcgtac gacggcagcg cgcgcccgat
     6181 gttcgcgatg ttgatcgcca ggttggcgaa ccgccgcgta tggccgatgt acaggccgat
     6241 cggcaggccg acgaccgttg ccacgaggat cgacgcaccg gacagcagga tgtgctcgag
     6301 gaggcgctgc tggatgctgc cggtgccgct gaaatgggcc ggatcgacga accacccgat
     6361 gaggtcgctc atcaggccgt tcccgcccgg gtgcgggccc agggtgtgac gaggcgctcg
     6421 atccagacca gcaggagatc ggcgccgaac gccatcacga tcgacaggac ggcgccgagg
     6481 taaatctcgg tcaggaagaa ccgatcaagc ccctcggtga tgaagaaccc gaagccgccg
     6541 aatgtttcac cgaggatgga ggcgacggtg gcgaggccga tggtggtgac cgtcgcgagg
     6601 cggattcccg cgatcatcaa gggcagggcc agcggaagct cgaccctgag gagacgttgc
     6661 cgcctcgtgt accccatgcc atcggccgcc tcgaggacct cggccgggac cgagttgaag
     6721 cccgcgacgt tcgcgcgaac gaggatgagg agcgtgtagg tcgtcagcgg gatgagcgcg
     6781 gtgacgatcg tgaggcccgt gatcgggcgc aggatcgcaa acgcggcgag gctcgggatc
     6841 gtgtagagca gtccggtgat cgcggtgagc ggtggataga cgcgcggctg gcgataggcc
     6901 cagatcgcga ggacgagcga gatcacgaag ccgagcgtca cggcgatcgc gacgagctgg
     6961 aaatgctgga tgaaccgcag gcccatcgcc ccgacgtggg tcgacagcca gccccagtcg
     7021 atcgagatgg tcacggctca ggcgctcgcc gccgacgctg ccgggtcggc atcctgatcg
     7081 tccgggggcg gctcatcgat ctcgccggtc tccgccgccg ggacctggtc gaaggcggcg
     7141 gcgtgctcgc cctcgcgcat cttctcggcg atcgtggcga tcgacaggag gccctggacc
     7201 gcgccggtcc ggtcgacgac gacgccggtc tggacgtcgg cgtcgagcat catcgagagg
     7261 gcgtccttga gcgtcgtccg cttgttcagc tggggcgagc tcgggttggc catcgcctcg
     7321 gtgagggttc cgtcggcggg gatgtcgccg tgggcgaccc agccgatcgg cttgttgccg
     7381 gcgtcgatca ggagcaggta gcggagcggg tcgtcgaggg cacggcggcg ggcatcggcg
     7441 gcggcgtcgc cgggtcgggc ggtgacggcg aggcggatcg ggacgtcgga tacccggagc
     7501 agggacagcc gcttcaggcc gcggtcctgg ccgacgaagc gggcgacgaa ctccgacgcg
     7561 ggcgcggcaa ggatgtcgag cgggcgtccg aactgggcga ggatgccgcc ggtctggaag
     7621 acggcgacga ggtcgcccat cttgatcgcc tcgtcgatgt cgtgggtgac gaagaggatc
     7681 gtcttggcga tcgtctcctg gagccgcagg aattcgttct ggaggcggtc gcggacgatc
     7741 gggtcgacgg caccgaaggg ctcgtccatg agcatgatcg gcggatcggc cgcaagcgcc
     7801 cgggcgacgc cgacgcgctg gcgctcgccg cccgacagct ggctcgggaa ccggtcgcgg
     7861 tacttcgccg ggtcgagccc gacgaggccc agaagctcct cggaacgctc cttgaggcgg
     7921 gctttcggcc agccgaggag ccgcggcacg gtcgcgacgt tctcgccgat cgtctggtgc
     7981 gggaacaggc cgacctgctg gatgacgtag ccgatgctcc gccggagctc ggtcagctca
     8041 cgcgtcccgg cgtccacgcc gtcgatgacg atcttgcccg ccgtcggctc gatcaggcgg
     8101 ttgaccatct tgagcgaggt cgtcttgccg cagccggacg ggccgacgag gatgcagatc
     8161 ttgccggctg ggaccttcag cgacaggtcg ttcacggcac cgggcgtcgg cttgcccttg
     8221 tccttgccgg tcggcacgtc gtagcgcttc gtcacgtggt cgaactcgac gaccgcggcc
     8281 tggcgatgct ggtcggccat cggcgctgcg atcctcctgg cgggcgggtc ggacgtggtt
     8341 tcgcgggtga gcgccgacag taccgcatcg cggatcgtca gcgatactgc ctcccacatg
     8401 aggatctccg ccatccggat cgaacggctg agggtgccgc tcgacccgcc gttccc
//
LOCUS       DATAIW010000212         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_11040, whole genome shotgun sequence.
ACCESSION   DATAIW010000212 DATAIW010000000
VERSION     DATAIW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11040"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHM48_06755"
     CDS             <1..313
                     /locus_tag="VHM48_06755"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX2755144.1"
                     /translation="SAPFREALAEVAIVIDDEPTAQQRRENDLADDEMLYGLYEGVPR
                     TEYGADWAGVPNRITLFRLPLEEDFADPAELADEVRITVIHELAHHLGIDDDRLDELG
                     L"
     gene            complement(328..1365)
                     /locus_tag="VHM48_06760"
     CDS             complement(328..1365)
                     /locus_tag="VHM48_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX2755145.1"
                     /translation="MTTADRLVGLDDILASRVRIAARVHRTPLLSSATAARFVAESGG
                     PRLADGRLYLKAEHLQKTGSFKARGMTNRIASLDPAQRARGAITMSAGNAGQAYAWAG
                     HEAGVPVTVVMAVGANPTKVAACRGYGAEVVLEGEHVGEAFQALERIRDERGLTFVHP
                     FEDAPVIAGHGSVGLEILDDLPEVDLVVVGVGGGGLISGTAAALKERRPAIRVYGVEP
                     VDAAAMSLAFEAGAPVTIQPRTVADGLAAPFAGILTLAMCRRYLDGLVLLDDPTILAG
                     VRFALERMKQVLEPAGAAALAAVLFGRIPIADGERVAVVASGGNIDVARLSELLAAAA
                     PLPGPGPAPST"
     gene            1465..1779
                     /locus_tag="VHM48_06765"
     CDS             1465..1779
                     /locus_tag="VHM48_06765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2755146.1"
                     /translation="MLDTAPLVSSSEFDRQRYAAVGRALADPKRLCVLESLAAGEVSV
                     SDLSTRVGCQVPNMSQHLAVLRSAGLVQSRRDGSTVFYRLADVRVLEAYRLIQSLAGQ
                     GG"
     gene            1842..2201
                     /gene="trxA"
                     /locus_tag="VHM48_06770"
     CDS             1842..2201
                     /gene="trxA"
                     /locus_tag="VHM48_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2755147.1"
                     /translation="MSIQHATDDTFEQLVLKNDKPVVVDFWATWCGPCKMVAPEMEKI
                     AAKYEGSVDVVKVDVDANPRISQAFNIMSIPTIAFFRPGQQPMGVAGARPMEALEAQF
                     GLAQYATEAPKTAATEA"
BASE COUNT          363 a          828 c          791 g          308 t
ORIGIN      
        1 gtcggcgccg ttccgtgagg ccctcgcgga ggtcgcgatc gtcatcgatg acgagccgac
       61 cgcgcaacag cggcgcgaga acgacctcgc cgacgacgag atgctctacg gcctctacga
      121 gggcgtgccg cggacggaat acggcgccga ctgggcgggc gtgccgaacc ggatcaccct
      181 tttccgactg ccgctcgagg aggactttgc ggacccggcc gagctggccg acgaggtccg
      241 gatcaccgtc atccacgagc tcgcccacca tctcgggatc gacgacgacc ggctcgacga
      301 gctcggcctc tgatcggggc gccgcggtca ggtcgacggc gcaggtccgg gtcccggcag
      361 cggcgccgca gccgccagca gctcactcag gcgtgcaaca tcgatgttgc cgcccgacgc
      421 gacgacggca acccgctcgc catcggcgat cgggatccgc ccgaacagga ccgccgcgag
      481 ggccgccgcg ccggccggct cgagcacctg cttcatccgc tcgagggcga accggacgcc
      541 ggccaggatc gtcgggtcat cgagcaggac gagcccgtcg aggtagcgcc ggcacatcgc
      601 gagggtgagg atgccggcga acggcgcggc caggccatcg gcgaccgtcc ggggctgaat
      661 cgtcacgggc gcgccggcct cgaaggcgag gctcatcgcc gccgcgtcga ccggctcgac
      721 gccgtacacg cggatggcgg gccgacgctc cttgagggcg gccgcggttc ccgagatcag
      781 cccgccaccg ccgacgccga cgacgaccag gtcgacctcg ggcaggtcgt cgaggatctc
      841 cagaccgacc gagccgtgac cggcgatgac cggagcatcc tcgaacgggt gaacgaacgt
      901 caggccgcgc tcgtcgcgaa tccgctcgag agcctggaat gcctcgccga cgtgctcacc
      961 ctcgaggacg acctcggcgc cgtagccgcg acaggcggcg accttcgtcg ggttggcccc
     1021 gaccgccatg acgaccgtga ccgggacgcc ggcttcgtga ccggcccagg cgtaggcctg
     1081 cccggcattc cccgccgaca tcgtgatcgc gccgcgggcc cgctgcgccg gatcgagcga
     1141 ggcgatccgg ttcgtcatcc cgcgggcctt gaacgagccc gtcttctgga gatgctctgc
     1201 cttcaggtag agccggccgt cggcgagccg cggcccgccg gactcggcca cgaaccgcgc
     1261 cgctgtcgcg ctcgacagga gcggcgtccg atggacccgg gcggcgatcc gcacgcgtga
     1321 ggccaggatg tcgtcgagcc cgacgaggcg gtcggcggtc gtcatcggcc gagtatgccg
     1381 tcctcgggcg ggacgggagt cagaaccgca cgattgccta ttccatactt gcgtgtttgc
     1441 gcaaacagtg acatgatcga cgcgatgttg gacaccgccc ctctcgtctc gtcgagcgag
     1501 ttcgatcgcc agcggtacgc cgcggtcggt cgggcgctgg ccgacccgaa gcgcctctgc
     1561 gtgctcgagt cgctggccgc cggcgaggtg tcggtgagcg atctctcgac gcgggtcggg
     1621 tgccaggtgc ccaacatgag ccagcacctc gccgtcctcc gcagcgcggg tctcgtccag
     1681 agccggcgcg acgggtcgac ggtcttctac cggctcgccg acgtccgcgt cctcgaggcc
     1741 tatcggctca tccagagcct tgccggccag ggcggctgac gaaccccgat cgtccgaagc
     1801 agcacccgcg cgcatctcgc gggaagaaag gaaccaaccc catgtccatc cagcacgcga
     1861 cggacgacac cttcgagcag ctcgttctca agaacgacaa gccggtcgtc gtcgacttct
     1921 gggccacctg gtgcggacca tgcaagatgg tcgccccgga gatggagaag atcgccgcca
     1981 agtacgaggg ctcggtcgac gtcgtcaagg tcgacgtcga cgccaacccc cggatctcgc
     2041 aggcgttcaa catcatgagc atcccgacga tcgccttctt ccggcccggc cagcagccga
     2101 tgggcgtggc gggcgcccgg ccgatggaag cgctcgaagc ccagttcggc ctggcccagt
     2161 acgcgaccga ggctcccaag accgccgcga cggaggcctg accgcccgaa ctcgctgcaa
     2221 cagcgaccga cgccgcccgg atccgggcgg cgtcggtcgc tgttgcagcg agttcgggcg
     2281 gtcaggcctc
//
LOCUS       DATAIW010000213         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_41237, whole genome shotgun sequence.
ACCESSION   DATAIW010000213 DATAIW010000000
VERSION     DATAIW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41237"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VHM48_06775"
     CDS             <1..417
                     /locus_tag="VHM48_06775"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX2755148.1"
                     /translation="IAAPRAKVWAFVIDPNQVGQCGPGVESIEVIDDTHFKATAKVGI
                     GFISARFIVNMEMAELTPPDQAIIKAHGQAPGSAVDATASMNLSDGENGGTRMDWSAD
                     VAIAGSLASLGARLIEGTANKMIGQTFDCMKSKLEA"
     gene            435..>1933
                     /locus_tag="VHM48_06780"
     CDS             435..>1933
                     /locus_tag="VHM48_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2755149.1"
                     /translation="MGSAAHPARLLGGLADAVPHLVWVATPDGVVREYSRRIEAYAAA
                     GVETDDGWRWESMVHPDDVPGTVAAWSAALAGGSEYEHEHRLRMADGTWRWHLSRGIA
                     MPAPGGEGSLWYGTATDIHAVRDAEERLRRTQSALAVAMRGGRMGWWQRDLATEVVTW
                     SPELEALFGLEPGAFQGDESRFLSLVHEDDRPAIGAAVAEAIATGGDYVVEFQFQRSD
                     ATWGWMEGRGRATYTDGRPSVLSGIGIDITERKQAEEAIRAREERLRLAAEAGEFGLY
                     DYDLEHGGMYWSPELQRILGTDGASSPEDAAIHPDDREATLERFAAAQSPLSGGSFDF
                     EYRMVRPDESIRWVSTHGQTYFSEPEPSPTRRPLRSIGVVVDVTERKQADELRDVFVG
                     MLSHELRTPVTAIYGGSQVLRREHIDEATRQEIIADIISESERLERLVENLLVLARAE
                     RHAVVGGQDPVLIRPILTRVVAEKRRRWPGSVLEVEVEPGLPPVTGDDAS"
BASE COUNT          292 a          660 c          713 g          268 t
ORIGIN      
        1 atcgccgcgc cccgcgccaa ggtctgggcg ttcgtcatcg atccgaacca ggtcggccag
       61 tgcggccccg gcgtcgagtc gatcgaggtc atcgatgaca cccatttcaa ggcgaccgcc
      121 aaggtcggga tcgggttcat cagtgcccgt ttcatcgtga acatggagat ggccgagctg
      181 acaccgccgg accaggcgat catcaaggcc cacggccagg cgcccggcag cgcagtcgat
      241 gccacggcct cgatgaatct cagcgacggg gagaacggcg gcacccggat ggattggagc
      301 gcggatgtcg cgatcgccgg gagcctcgcc tcgctcggtg cgcggctcat cgagggcacg
      361 gcgaacaaga tgatcgggca gacgttcgac tgcatgaaat cgaagctcga ggcttgacgg
      421 aggcgggctc gaccgtgggc tccgcggcgc acccggcgag gctcctgggt gggcttgccg
      481 acgcggtccc gcacctggtc tgggtggcga cgccggacgg cgtcgtgcgt gagtacagcc
      541 gccggatcga ggcctacgcc gccgccggcg tcgagaccga cgacgggtgg cgctgggagt
      601 cgatggtcca tcccgacgat gtcccgggca ccgtcgccgc ctggtccgcg gcgctcgccg
      661 gagggtcgga atacgagcac gagcatcggc tgcggatggc cgacggaacg tggcgctggc
      721 acctgagtcg cgggatcgcg atgccggccc cgggcggcga gggctccctg tggtacggga
      781 cggccaccga catccacgcc gtgcgcgacg ccgaggaacg gctgcggcgc acccaatctg
      841 ccctggcggt cgcgatgcgc ggcggccgga tgggctggtg gcagcgcgac ctcgccaccg
      901 aggtcgtgac gtggagcccg gagctcgagg cgctcttcgg cctggagccg ggcgcgttcc
      961 agggcgacga gagccggttc ctgtcgttgg tccacgagga tgaccggccc gcgatcggag
     1021 ccgccgtcgc cgaagcgatc gccaccggcg gcgattacgt cgtcgagttc cagttccagc
     1081 ggtccgatgc cacctggggc tggatggagg gtcggggccg ggccacgtat accgacggcc
     1141 ggccgtcggt gctctccggc atcggcatcg acatcaccga gcgcaagcag gccgaggagg
     1201 cgatccgcgc ccgggaggag cgcctccggc tcgccgccga ggcaggcgag ttcggcctgt
     1261 acgactacga cctcgagcat ggcgggatgt actggtcgcc cgagctccag cggatcctgg
     1321 gcacggacgg cgcgagctcg cccgaggatg cggccatcca tccggacgac cgcgaggcga
     1381 ctctcgaacg tttcgccgcg gcgcagtcgc ccctgagcgg cggatcgttc gacttcgagt
     1441 accggatggt ccggcccgac gagagcatcc gctgggtctc gacccatggc cagacctact
     1501 tctcggaacc cgagcccagc ccgacccggc ggccgctccg ctcgatcggg gtcgtcgtgg
     1561 acgtcacgga gcggaagcag gccgacgagc tccgcgacgt cttcgtcggg atgctcagcc
     1621 acgagctccg gacgcccgtc accgcgatct acggcgggag ccaggtcctc cgtcgtgagc
     1681 acatcgacga ggcgacgcgg caggagatca tcgccgacat catcagcgag agcgagcgtc
     1741 tcgagcggct cgtcgagaac ctcctggtcc tcgcccgggc cgagcggcat gccgtcgtcg
     1801 gtggccagga cccggtcctg atccggccga tcctgacccg ggtcgtcgcg gagaagcggc
     1861 ggcgctggcc gggctccgtc ctggaggtcg aggtcgagcc gggtctgccg ccggtcaccg
     1921 gcgacgatgc ctc
//
LOCUS       DATAIW010000214         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_124137, whole genome shotgun sequence.
ACCESSION   DATAIW010000214 DATAIW010000000
VERSION     DATAIW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124137"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHM48_06785"
     CDS             <1..622
                     /locus_tag="VHM48_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX2755150.1"
                     /translation="TINRMNDLVSTFRLEGSILLDGQNLYADGVDAVELRRRVGMVFQ
                     KPNPFPKSIFDNVAFGARINGYTGNIGELVEESLRRAALWDEVRDKLKQSGLSLSGGQ
                     QQRLCIARAIATEPEVILMDEPCSALDPRATLQIEELMAELKTQYTIVIVTHNMQQAA
                     RASDRTAFLTMGDDRAGYVVEQDRTVEIFTNPKNQLTEDYVSGRFG"
     gene            648..1412
                     /gene="phoU"
                     /locus_tag="VHM48_06790"
     CDS             648..1412
                     /gene="phoU"
                     /locus_tag="VHM48_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2755151.1"
                     /translation="MTDDRPPNTSQREASQAVAEALTVAADAVAHMNEAHPPRPGLDQ
                     EMRQVKDGILRMGSLVEEAIRAAIDALARHDAAAALAVIIADSRINEMQRDVSAMITK
                     AIATQSPVARDLRFLLSLDHVGYELERMGDHAASVAKQVRKLAPEPPLKRYVDLPAMG
                     ELAATLVGEILRALVDIDVVAAREVAARDDDIDLLYHRTFDEVVNLMRADPDNVERGT
                     RILFAAHYLERIGDRVTNIAEDVVFLASGQIEDLNP"
     gene            1409..1858
                     /locus_tag="VHM48_06795"
     CDS             1409..1858
                     /locus_tag="VHM48_06795"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX2755152.1"
                     /translation="MSGRPIRVLFVCTANASRSILAEALLRRVGGDAYEVHSAGIEPT
                     VVHPLTRRVLEAAGFDHEWAAAKHVNDFAGQPFAYVVTLCDDARLVCPIFPGADQSMH
                     WGYPEPASVEGDEAARLLAFERVFTSLAERVRQFVVVTGRKASAVPG"
     gene            1865..2305
                     /locus_tag="VHM48_06800"
     CDS             1865..2305
                     /locus_tag="VHM48_06800"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX2755153.1"
                     /translation="MGDADAAPIRILVLCTGNSCRSILAEALFRELGGPRIAIESAGS
                     QPAGAVNPRTIQVLDEAGIDHAWARSKSMTEFLDQEFDHVLTVCDDAAEACPVFPGPA
                     VRTHWSIPDPAGATGTEEEQVTVYRATLADLRGRIAPFLATLER"
     gene            2302..2814
                     /locus_tag="VHM48_06805"
     CDS             2302..2814
                     /locus_tag="VHM48_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2755154.1"
                     /translation="MSPTDRRHDLQLHLLRHADAGDPAGWAGDDALRPLSEKGELQAE
                     RLGSFLAEIGFQSDAIVSSPKVRARRTAEIVGGAIGVGVQLDERLAGGFDPETLDAIL
                     ADAGNPGRPVVVGHDPDFSQVLGWLADADGLTMKKGAFARVDVRGVVADGRGTLRWLV
                     PPDLLDRERR"
     gene            complement(2818..3180)
                     /locus_tag="VHM48_06810"
     CDS             complement(2818..3180)
                     /locus_tag="VHM48_06810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2755155.1"
                     /translation="MPRAKTTLIDPDVQLLQALGHPTRLSIVRELTGGGEVCACDFTS
                     CCDVSQPTVSHHLRILKEAGVIEPERRGTWIYYRIVPSAADRLRALAGELLGAPQVIP
                     AGTLRRTSRGPAAPSARA"
     gene            3265..3459
                     /locus_tag="VHM48_06815"
     CDS             3265..3459
                     /locus_tag="VHM48_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755156.1"
                     /translation="MDTNLAAWMIAGGPRIELKATQRDREQLYALRESQRVERVSLID
                     RLRGIARARQADTDLVCCPA"
     gene            3503..4381
                     /gene="arsM"
                     /locus_tag="VHM48_06820"
     CDS             3503..4381
                     /gene="arsM"
                     /locus_tag="VHM48_06820"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX2755157.1"
                     /translation="MLDQAATTSDQDRIHDEVRARYAEAAVAAELHRSDSAAQASCCG
                     PDGTAVFGEILYDAADRAQLPEAAVLASLGCGNPIAVADLRPGERVLDLGSGGGIDVI
                     LSARRVGPTGRAIGLDMTDEMLALAQRNAAEAGLDNVEFVRGTIESIPLPAASVDVVI
                     SNCVINLAADKAAVFAEIARVLRPGGRVGVSDVVADDTLSPADRAERGSYSGCIAGAL
                     SFAEYRAGLAAVGLVDVSIEPTHAVADGLHGAIIKATRPLEPVAELPAMPVLPRAVAM
                     LDASAGGCGCGSEGCC"
BASE COUNT          677 a         1569 c         1517 g          658 t
ORIGIN      
        1 gacgatcaac cggatgaacg acctcgtctc gaccttccgc ctcgagggca gcatcctgct
       61 cgacggccag aacctgtacg ccgacggcgt cgacgcggtg gagctccgcc gccgggtcgg
      121 gatggtcttc cagaagccga accccttccc caagtcgatc ttcgacaacg tcgcgttcgg
      181 agcgcggatc aacggctata cgggcaacat cggcgagctc gtcgaggaga gcctccgtcg
      241 cgccgccctc tgggacgagg tcagggacaa gctcaagcag agcggcctgt cgctgtccgg
      301 cggccagcag cagcgcctgt gcatcgcccg ggcgatcgcg accgagcccg aggtcatcct
      361 catggacgaa ccgtgctctg cgctcgatcc gcgggcgacc ctccagatcg aggaactgat
      421 ggccgagctg aagacgcagt acacgatcgt cattgtgacc cacaatatgc agcaggccgc
      481 gcgagcctcc gatcggaccg cgttcctgac gatgggcgat gaccgggccg gctatgtcgt
      541 cgaacaggac cgaacggtgg agatcttcac caatccgaag aaccagctga ccgaggacta
      601 cgtctcggga cggttcgggt gagcggatcg caaacgaggg ccgaccgatg actgacgacc
      661 gaccgcccaa cacatcgcag cgcgaggcgt cccaggcggt cgccgaggcg ttgacggtcg
      721 cggccgatgc ggtggcccat atgaatgagg cgcatccgcc ccgccccggc cttgaccagg
      781 agatgcgcca ggtcaaggac gggatcctcc ggatgggctc cctggtcgag gaggccatcc
      841 gggccgcgat cgacgctttg gcgcgacacg acgcggcggc tgccctggcc gtgatcatcg
      901 ccgactcgcg gatcaacgag atgcagcggg acgtctcggc catgatcacc aaggcgatcg
      961 ccacccaatc gccggttgcc cgggatctcc gcttcctgct gtccctggat cacgtgggtt
     1021 acgagcttga gcggatgggc gaccacgccg catcggtcgc gaaacaggtt cgcaagctcg
     1081 cgccggagcc gccgctgaag cgctatgtcg acctgccggc catgggcgag ctcgccgcca
     1141 cactcgtcgg agagatcctg cgggccctcg tcgacatcga cgtcgtggcc gcccgtgagg
     1201 tggccgcgcg cgatgacgac atcgacctgc tctaccaccg gacgttcgac gaggtcgtca
     1261 acctgatgcg ggcggatcct gacaacgtcg agcgcggcac gcggatcctc ttcgccgccc
     1321 actacctcga gcggatcggc gatcgagtca ccaacattgc cgaggatgtg gtgttcctcg
     1381 ccagcggcca gatcgaagac ctcaacccat gagtggccgg cccatccggg tgctgttcgt
     1441 gtgcaccgcc aacgcgtcgc gctcgatcct cgccgaggcg ctcctgcggc gtgtcggcgg
     1501 cgacgcgtac gaggtccatt cggccggcat cgagccgacc gtggtccatc cgctcacccg
     1561 ccgggtgctc gaggcggcgg ggttcgacca tgagtgggcc gcggccaagc acgtcaacga
     1621 cttcgccggc cagcccttcg cctacgtcgt gaccctctgc gacgacgccc ggctcgtctg
     1681 cccgatcttc cccggtgccg accagtcgat gcactgggga taccccgagc cggcctcggt
     1741 cgagggggac gaggccgccc ggctgctcgc cttcgagcgg gtgttcacgt cgctcgccga
     1801 gcgcgtccgt cagttcgtcg tggtcacggg tcgcaaggcg tcggccgtcc cgggctgatc
     1861 ccgaatgggt gacgcggacg ctgcgccgat ccggatcctc gtcctgtgca ccggcaacag
     1921 ctgccgctcg atcctggctg aggcgctgtt ccgcgagctc ggcggtccgc ggatcgcgat
     1981 cgagtcggcc gggagccagc cggcgggtgc cgtcaatccg cgaacgatcc aggtcctcga
     2041 cgaggccggg atcgaccatg cctgggcccg ctcgaagtcg atgaccgagt tcctcgatca
     2101 ggagttcgac catgtcctga ccgtctgcga cgatgccgcc gaggcctgcc cggtcttccc
     2161 gggcccggcg gttcggaccc actggagcat ccccgatccg gccggggcca ccggcaccga
     2221 ggaggagcag gtcacggtct accgggcgac cctggccgac ctccgcgggc ggatcgcgcc
     2281 attcctcgct accctcgaga ggtgagcccg accgaccgcc gccatgacct ccagctccat
     2341 ctcctgcgcc atgccgatgc cggcgatccc gcgggctggg ccggcgacga cgcgctccgg
     2401 ccgctgagcg agaagggcga gctccaggcg gagcggctcg gctcgttcct cgccgagatc
     2461 ggtttccaat cggacgcgat cgtcagctct ccgaaagtcc gggcgaggcg cacggcggag
     2521 atcgtcgggg gcgcgatcgg ggtcggcgtc caactcgacg aacggttggc cggcgggttc
     2581 gatccggaga ccctcgacgc gatcctggcc gacgcgggca acccgggccg gcccgtcgtc
     2641 gtcggccacg atccggactt cagccaggtc ctcggctggc tggccgacgc cgatggcctg
     2701 acgatgaaga agggcgcctt cgctcgggtc gacgtccgcg gggtggtggc cgacgggcgc
     2761 gggaccctgc gctggctcgt gccgccggac ctgctcgacc gcgagcggcg ctgacagtca
     2821 ggcgcgcgcg gacggtgcag cggggccgcg gctggtcctg cggagggtgc cggccgggat
     2881 gacctggggc gcaccgagga gctcgccggc gagcgcccgg agccgatccg ccgccgacgg
     2941 cacgatccgg tagtagatcc aggtcccccg ccgctcaggc tcgatcacgc ctgcttcctt
     3001 gaggatccgg agatggtgcg agaccgtcgg ctggctgacg tcgcagcacg aggtgaagtc
     3061 gcaggcgcag acctcgccgc cgccggtcag ctcgcggacg atcgacagcc gggtcgggtg
     3121 gccgagcgcc tggaggagct ggacatcggg gtcgatcagg gtcgtcttcg cacggggcat
     3181 gagggcattc tatcgacgcc gatctatatt gacaactatc aatatatggc gctaggatga
     3241 gcacatacca gaaaggagtg ccccatggac acgaatcttg ccgcctggat gatcgccggc
     3301 ggcccccgga tcgagctcaa ggcgacgcag cgcgatcgag agcagctgta cgccctccgc
     3361 gagagccagc gcgtcgagcg cgtcagcctg atcgaccgcc tgcgtggcat cgcccgggcc
     3421 cgccaggccg acaccgacct ggtctgctgc cccgcctgat cgccagaccg cgccacaacg
     3481 ccacccgagg agacccacca cgatgctcga ccaggccgcc acgacctccg accaggaccg
     3541 gatccacgac gaggtccgcg cccgttatgc cgaagccgcc gtcgccgctg aactccaccg
     3601 ttccgattcg gccgcccagg cttcgtgttg cggacccgac ggaacggcgg tcttcggcga
     3661 gatcctgtac gacgcggccg atcgcgccca gcttcccgag gcggcggtcc tcgccagtct
     3721 cggctgcggc aatccgatcg cggtcgccga tcttcgaccg ggcgaacggg tcctcgacct
     3781 cggctcgggc ggcgggatcg acgtgatcct ctcggcccgc cgggtcggcc cgaccggtcg
     3841 tgcgatcggg ttggacatga ccgacgagat gctcgccctg gcccagcgca acgctgccga
     3901 ggccggtctc gacaacgtcg agttcgttcg cggcacgatc gaatcgatcc cgctcccggc
     3961 tgcctcggtc gacgtcgtca tcagcaactg cgtcatcaat ctcgcggctg acaaggcggc
     4021 ggtgttcgcc gagatcgccc gggtcctccg gcccggtggt cgggtcggcg tgagcgacgt
     4081 cgtcgccgac gacaccctca gcccggcgga ccgcgccgag cgcggctcct acagcggctg
     4141 cattgccgga gccctctcct tcgccgagta ccgggcgggc ctggccgcgg tcggactggt
     4201 cgacgtgtcg atcgagccga cgcacgcggt cgccgacgga ctccatggcg cgatcatcaa
     4261 ggcgacccga ccgctcgagc ccgttgccga gctgccggcg atgccggtcc tgccgcgggc
     4321 cgtcgcgatg ctcgatgcat cggccggcgg ctgcggctgc ggctccgagg gctgctgctg
     4381 agcctctacg gggctcgtcg gggctcagca gcagccctcg g
//
LOCUS       DATAIW010000215        28737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_100830, whole genome shotgun sequence.
ACCESSION   DATAIW010000215 DATAIW010000000
VERSION     DATAIW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 28737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28737
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100830"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHM48_06825"
     CDS             complement(<1..204)
                     /locus_tag="VHM48_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755158.1"
                     /translation="MRSADADWTDRTVPLTVRDADVDDAASVRDVAEAAWRDTYAGLL
                     SASTIETFIDGAYSIDRLQRRIDR"
     gene            complement(185..652)
                     /gene="smpB"
                     /locus_tag="VHM48_06830"
     CDS             complement(185..652)
                     /gene="smpB"
                     /locus_tag="VHM48_06830"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX2755159.1"
                     /translation="MADEKTIALNRRARHDFTIDETFEAGIVLTGTEIKSIRAGKVNL
                     SDAYARIERDEAWLIGAHIAPFEQANRYNHEPKRIRKLLLHRSEIDELLGRTKAKGQT
                     IVPLRLYLTGKGRAKLELGLARGKNLYDRRRDIADRDAKRDIAREMADAQRGR"
     gene            complement(971..2404)
                     /locus_tag="VHM48_06835"
     CDS             complement(971..2404)
                     /locus_tag="VHM48_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2755160.1"
                     /translation="MLPSDSSVPDPGLTTDPAVGPPSDALDADHADPAGAGPIDAVPT
                     QPVPAPAGGRPSSSVRGPILAIAMVVVSVLAGGALFVSGFLVGQRSIEQPGTPVASEQ
                     AFQPFWDSYDTITKRFALGGETQQSLIDGAIKGMVDSLGDPYSAYLTPDQYSQGLQDL
                     SGQFEGIGAEIGTHDAKGVSSDCTTLGPDCQLVVVTPIEGSPSEKAGVKAGDTIVAVD
                     GTSLDGLTVDGARNKIRGKKGTEVVLSIVRDKAAPIDIKVVRDVIVSKEVIARDLAEG
                     TIGYIAVTGFSDNSQTKFHDALQADLKAGKKAIILDLRGDPGGYVTAARSIASEFIKD
                     GPIFWEQDADGAQVETDSTGQGIATDASIKLIVLVDKGSASASEIVAGALQDRKRATI
                     VGETSFGKGTVQQWIQLQNGSALKLTIAKWLTPDKHWIHHVGIIPDVPVATPADAGPT
                     NDPVLDKAVELLTKASASLPSALSRAA"
     gene            complement(2536..3426)
                     /locus_tag="VHM48_06840"
     CDS             complement(2536..3426)
                     /locus_tag="VHM48_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2755161.1"
                     /translation="MIGFVVFSLRRAWQGFWRNALMSLAATATMVLMLLLLSGFWIIQ
                     TGLLSALDFTESKVEVQVNMKVDASQVDITNLATTIKALPGVAGATLVTREEALARFQ
                     QSMKNQGREDLSKVLDSNPFPPSYEIKLTDPSFASPVAAALQPNLNPAVRNVLDTKDT
                     AKNVKTVTDIMRTAGSVILVVIGVIVLFIIVNTIRLAVVARAEEIEIMRLVGASDAFI
                     RWPFVFEGAMVGLFGAAITLIGLYFLADPIGGFMVDFFRVLPLSFGSLARDLVVIVTS
                     AGLGLGVLGSWLSVRTYLIR"
     gene            complement(3490..4269)
                     /gene="ftsE"
                     /locus_tag="VHM48_06845"
     CDS             complement(3490..4269)
                     /gene="ftsE"
                     /locus_tag="VHM48_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX2755162.1"
                     /translation="MTLAPVPTATTRSVRERLLRRGRDRSAVDRTVLVLHDVTKTYPN
                     GKTALRDVDLVIPEGDFVFLVGPSGAGKSTIIKLLIRDEIATQGQVILDGHDLAKLPR
                     RAVPKVRRKIGIVFQDFKLLPTKNVWENVAFALEVTGSPRRKIRPAVDRVLALVGLTA
                     QRNQIPSQLSGGEQQRTAIARALVHDPRLIIADEPTGNLDPLISWEIIQLLLRINELG
                     VTVLMATHDADVVTALRKRVVALEEGRVIRDEVGGAYHRSD"
     gene            complement(4409..5428)
                     /locus_tag="VHM48_06850"
     CDS             complement(4409..5428)
                     /locus_tag="VHM48_06850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755163.1"
                     /translation="MTAGPRGGTEILTTSGHRIRRATPDDLPTCAAIWRESLNDYLGR
                     LAQPEIPDDLASLLRLYGHLHATDPTTFLVAAAPEARPSGDGTGEPALIDAFVVVVRR
                     DDLWYLSMLFVRPAAQARGLGRALLAAVAPEGSSDSSASVTRATSTDTAQPISNGLYA
                     SLGMVPRIPLLRLVGLPTRDGGFGPLPAGVEAIAFAEVGDAGDGLGRSALAAELDALD
                     RDAAGFSRTADHRFHAREGRIGFLFRDRGGRAVAYGYSSEAGRVGPIAVREAALLGPV
                     VGHLVHEVRPRGAFGLWVPGTAGEAVVPLLRAGFRFDGFPVLLCWDRPFADFSRYIPM
                     SPGLL"
     gene            complement(5425..6504)
                     /gene="prfB"
                     /locus_tag="VHM48_06855"
     CDS             complement(5425..6504)
                     /gene="prfB"
                     /locus_tag="VHM48_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX2755164.1"
                     /translation="MFDLEVKERRVSQLEALTSEPGFWDDQRSAQKVLREANGLRSDV
                     ELWRGFEGRADDLLTTLELVEESGDAELTAELDREVAALEADFGRERTALLFSGEYDQ
                     GGAILTISAGAGGTEATDWAEMLLRMYLRWAERHRFKTEIVDQQEGEQAGIKSATVEI
                     DGRAAYGWMRAERGVHRLVRISPFDSQKRRQTTFALVEVLPEADDDIEIELNWDEIRV
                     DTFRSQGAGGQHVNKTDSAVRLTHLPTSIVAQSQNERSQTQNKEMAIRVLKARLLERA
                     LEEQEAEVRKLKGEHVEAGWGNQIRSYVLHPYQMVKDLRTAHETGNTQAVLDGDLDAF
                     MQAELERLATGGGLAGGPPDEADEE"
     gene            complement(7017..7613)
                     /locus_tag="VHM48_06860"
     CDS             complement(7017..7613)
                     /locus_tag="VHM48_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX2755165.1"
                     /translation="MIRNLGRLLLAGIVAAVLVAGWASYRIWDVGNHDDRPRADAIVV
                     LGAAQYNGRPSVILKARLDHAIDLYRDGVARYLIVTGGKLDLDITTEAATARAYAIGR
                     GVPDAAILAEDRGRTTLESLRGAATIIHVRGLQTAIFVSDRTHMLRVLRIARDEGITA
                     YGSPTATSPSDLTFDDRLRSTAHELGGLTLYFVTGETP"
     gene            complement(7747..10746)
                     /gene="secA"
                     /locus_tag="VHM48_06865"
     CDS             complement(7747..10746)
                     /gene="secA"
                     /locus_tag="VHM48_06865"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX2755166.1"
                     /translation="MRFLSRFVDSNDREIRRIQPLVDEINELETEIAALSDEAIRERF
                     GLIREEIHEAAVPGEPTEEELTHPDLERRREMKKDRHKREQAALQAALDEVLPDVFAM
                     AREAMKRTLGMRHFDVQLMGGMVLHQGKISEMKTGEGKTFVPTLAAALNSLTGRGVHV
                     VTPNDYLARRDPQWMGPVFHFLGISLGMITHDESFVFEPDYPTSDERLINLRPVSRRD
                     AYAADVTYGTNNEFGFDYLRDNMVTDLADRVQGERSFAIVDEVDNILIDEARTPLIIS
                     GQAEESGDLYYTFARLVPKLTGRREGEEEGGDYFVDLKDKAVSPTEEGIDKIEKLVGV
                     ENLYDADPRLARHFEQALRAHALYRRDRDYIVKDGEIIIVDEFTGRQMPGRRWSEGLH
                     QAVEAKEGVKVQRESVTMATITFQNYFRLYDKLAGMTGTAMTEAEEFHKIYELEVVSI
                     PTHKDMIRDDEADLVFRNELGKFNALIDEIAEMNEAGRPVLVGTVSVEKSEVLSTMLQ
                     RRGIKHETLNAKFHEKESGIVAQAGRTGAITIATNMAGRGTDILLGGNPAGLASENLH
                     KRGLNPAEVDKETYAAALAEARTTTDEDHERVVGVGGLHIIGTERHDSRRIDNQLRGR
                     AGRQGDPGSSRFYLSLEDDLMKRFASDRVAGLMERMGLEDDVAIESRLVSKTIESAQS
                     RVEGFNFDMRKRVVEFDDVINRQRETIYAERDKVLRNEDLGETVRTFLDEDVRTLVAD
                     HLSGDTPDDWGMEALAAALKAMGLDGPGTSEDELWEIGSREEILEHLLDMAEARLEAR
                     AAEIGEADWSLVERHVLLRTIDSLWVEHLTEIDDMRRGIGLRGYAQQDPLNEFRREAF
                     ELYGELRGLIRHQVAATIFRVTVTRTPPPGESDLDQSLAAGARALTAAASGGSRPGAA
                     TSTSGSSATSAGGGRPAGPRPVGPVVAGGLPRDPMAAARLETSGNGRSVPAGQAKPGF
                     TPTGARIGRNDPCWCGSGQKYKKCHGR"
     gene            complement(11291..12064)
                     /locus_tag="VHM48_06870"
     CDS             complement(11291..12064)
                     /locus_tag="VHM48_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2755167.1"
                     /translation="MRSRPALLAIVVILLLAVGGGAATWALFLRGDNVAPLSLPSRAP
                     TSSSGSAAASPAAGASGATTSLPPASAAAVDAASMPGTWTIGADSVVGYRVRERLASL
                     SADSDAVGRTSTITGTATIAASGGTLSVAAADFSVDMTTIKSDRQMRDNRLRNDGIQT
                     DSFPTSTFKLTTPVALPAAATTGAQVSVTLHGDLTLHGVTKTVDIPAKAQLNGSLIQV
                     VGSLDFPFSDFAINAPNIGGFVAVEDHGTLEFLVNLAKS"
     gene            complement(12165..12416)
                     /locus_tag="VHM48_06875"
     CDS             complement(12165..12416)
                     /locus_tag="VHM48_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755168.1"
                     /translation="MSLQLHRPDGKGGVEPRPVSQPDWRRGMLSRRWGLNRRDGQLPE
                     MKNPEMNPTSTAMAVIFWVGLGALTFVILVAGYGIGFWH"
     gene            complement(12413..13540)
                     /locus_tag="VHM48_06880"
     CDS             complement(12413..13540)
                     /locus_tag="VHM48_06880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX2755169.1"
                     /translation="MRPLVREDAVYADQFEIYHGNSNPELARKIARWLGTECGKAEVF
                     QFANENIFVKILDNVREKDVFLVQPTSHPVNQSIMELLIMIDAFKRASAGRITAVIPF
                     YAYGRSDKKDQPRVPITARLIADMITVAGADRVLTMDLHQGQIQGFFNIPVDELTAVH
                     LLSNYFIHKHLTDVVVVTDLGFAKRARTFAELLDAPLAIIEKRRIGNLDRAELMNVIG
                     DVRGKRAIIVDDEIDTGGTLTEIVRALEREGVTEIYACATHGVLSDPAVDRIRDSNLL
                     EVVLTDSVPLPAIKRLPKVTTLSVAPLIGEAIKRIHRGESVGALFSSEVSFTQEMLLW
                     EDGQQRRMDTRQEPDHGSDPGADDDTEDDPVLLGATGQGDR"
     gene            complement(13548..16112)
                     /gene="gyrA"
                     /locus_tag="VHM48_06885"
     CDS             complement(13548..16112)
                     /gene="gyrA"
                     /locus_tag="VHM48_06885"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX2755170.1"
                     /translation="MTDDLGDIRAIRIEDEMRVSYLDYAMSVIVARALPDVRDGLKPV
                     HRRILYTMGEMGLSSTSSFRKCAAIVGEVMGKYHPHGDVALYDALVRLAQDFSMRYPL
                     VDGQGNFGSVDGDSAAAMRYTEARLTGIAAEMLDDIDKETVDYQDNYDGTQKEPSVLP
                     AKLPNLLINGSSGIAVGMATNIPPHNLNEICDATVALIDDPDITSDDLSRYVLGPDFP
                     TGGTIYRFEQQRNPLTGENETVDAIRHMYAHGRGRVVMRAQVAFEEVRGDRMAIIISE
                     LPYQVNKATLLEKIADLVKDKKIDGISDLRDESDRDGMRVYIEIKRDANPHKVLNNLF
                     KHTPMQLAFNMNMLALVDGQPQTLPLKSVLVHYVDHRRNIVRRRTEFDLGKARARAHI
                     LEGLKIALDNLDAVIRTIRESADVELARTNLMSRFDLSELQAQAILDMRLARLAALER
                     KKIEDEYLNVIQLIAELEDILANPARVLGIIKDELAELKKKFGGDRRTRVADDTSREM
                     TDEDLIADEDVVVTISGRGYIKRQPVATYRRQHRGGKGIIGHVTREEDAVEYLIVANT
                     HDWALFFTNRGRVFSAKVHTIVDASRQAKGTPIINLPGVQVEAGEVPVATIVLPNFEP
                     GHYLVMATRRGHIKKTPLEQFERVRSTGIRAVSIHDNDELAWVDVSSGADDVILATAQ
                     GMLARFHEDEVRPMGRDAAGVIGIRLLKKEGDSVVAMTVVEPGADLLVLSETGYGKRV
                     GLTEFRKKHRGGQGVRLIALEGRKTGLVAAVQQVTEEDEELLLISAGGQVIRTETNSI
                     NRYSSGARGVIVMRLAEGDRVVAIAAFRSGLAERDGIEDNGGPEPGGSGPVPGQDA"
     gene            complement(16170..17135)
                     /locus_tag="VHM48_06890"
     CDS             complement(16170..17135)
                     /locus_tag="VHM48_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX2755171.1"
                     /translation="MAPTMSPITARAVVFVADRRPDAEALGRALADELGDPERFADVL
                     RAGLAALADAEYLAGAQRIAPGIGDLHGVRWPLIEAVKRGFRDATRRERTSSWLFIAD
                     RLLREPQLEARWFAFGLLERLIVDDTERAWQLLRRAARDAGDWITVDSLAHPVGKGIL
                     NEPYRWAELEQLVYSPSRWERRLVGSTIATIPFVDRRRGRQPEVVTHGLGLVRELIGD
                     TAPDVQKALAWALRSMAAIDRAATTAFLDGEADLAASSSDGNRAWVVRDALPKLDPAD
                     ADRIRERLAGIRRRAGGPATSRASQTAQRFGAGLLGRPLPEPPLA"
     gene            complement(17135..17680)
                     /locus_tag="VHM48_06895"
     CDS             complement(17135..17680)
                     /locus_tag="VHM48_06895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2755172.1"
                     /translation="MDGERVTDEAGRVVTVPGSGLTIEVAPHHCFACGALNEHGIHLD
                     LHVDGDRCWTELILPDRFQGWDGIAHGGIICTILDEVMAWSLAATDNWGLTARMSVDF
                     KRPVRLGVAIRGEGWITAVRRRIVDTTARIVDPATGEVLATATATYVAADASRKAELQ
                     ARYRFRLAPASDGSRAGGSPA"
     gene            complement(17721..19694)
                     /gene="gyrB"
                     /locus_tag="VHM48_06900"
     CDS             complement(17721..19694)
                     /gene="gyrB"
                     /locus_tag="VHM48_06900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX2755173.1"
                     /translation="MTEEATQPQAATAGKTSRRTKAAGGSEYNASSIQVLEGLEAVRR
                     RPGMYIGSTDARGLHHLVWEVVDNSIDEAMAGHATTILVTVKVDGTVVVVDDGRGVPV
                     GKHSTGKDALEVVHTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSSWMRVESARDGT
                     VWSQEYERGKPKTAVIRVGPSGGRRGTTTAFRADADMFETTDYSFELISQRLRESAYL
                     TKGVWITLVDERVDRERSFYFEGGLISFVRHLNRNKEVLHQRPIYCEKREGSTAVEVA
                     LQYNDTYTENVLAFANNINTVDGGTHVTGFRAALTTSLNDWARRASVLKDNDANLSGD
                     DVREGLTAVVSVKMTDPQFEGQTKAKLGNAEVKGQVQTALAEALGQYLDENPGDGRRI
                     IEKCLTAARARDAARKARDLVLRKGALDGLSLPGKLADCQERDPARSELYIVEGDSAG
                     GSAKQGRDRRFQAILPLRGKLLNVEKARLDKIVASENVRPLIIALGAGIEGIGDSFDI
                     AKLRYHRIIIMTDADVDGAHIRTLLLTFFYRFMPQVIENGYLYIAQPPLYRVSTGKIT
                     KYAQSEKERDRIVKEMDRKNVSVQRFKGLGEMNADQLWDTTMNPETRTLLRAEIDDAA
                     TADAIFTMLMGEKVAPRKDFIKTEARKVRNLDV"
     gene            complement(19691..20554)
                     /locus_tag="VHM48_06905"
     CDS             complement(19691..20554)
                     /locus_tag="VHM48_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2755174.1"
                     /translation="MTVRSERQSGLGRGLSSLIPQRSQHAPTSEIPIARIRPNPRQPR
                     QRMDQGELDSLAASIREHGVLQPILVTETLDGYQLVAGERRFRASQQAGLERIPAVIR
                     QLADRDQLELALVENLQRADLGPMEEAHAYHSLASEFAMTHDQIAQVVGRAKSTITNT
                     IRLLDLEPSVQEALLDGRLSAGHARAIGGLPTEQQARVVGSVVDQGFSVRQTEELVRR
                     LREPRVAATPAASRRAVDPDLERVEDELRRHLGTKVTLARSRKGGRIIIEFYSDEELG
                     QLYDRLIGGPA"
     gene            complement(20551..21417)
                     /locus_tag="VHM48_06910"
     CDS             complement(20551..21417)
                     /locus_tag="VHM48_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755175.1"
                     /translation="MAQTIACANQKGGVGKTTTVVNLAAYLALAGERVLIVDLDPQGN
                     ATSGFGIDRSALEVTLYDVLLGNTAMAETVLPTVVSACSIVPASVSLAGAEVELATAD
                     QRERRLTKLLEPVLDAYDYVLIDCPPSLGLLTVNALTAADSVLIPTQCEYYALEGLTQ
                     LIATLNLVRDHLNPALAIKGVVLTMFDARTNLSADVAAEVRNHLGAAVFDTIIPRSVR
                     LSEAPSFGLPIALYRDNSRGAIAYAALAAELRRRDGHGSPITSTDLATPSGSPLLTST
                     ATTAALAVGVPG"
     gene            21830..22333
                     /gene="mreD"
                     /locus_tag="VHM48_06915"
     CDS             21830..22333
                     /gene="mreD"
                     /locus_tag="VHM48_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX2755176.1"
                     /translation="MTLLVAAIAATVAALLESTITQYLRVGDTAQPHLVFILAVIWTV
                     AAGLDSGLVWAFVGGIALDSLAQRPLGSTAFALLITVGATSVLARALTRVRPIVTIIA
                     TALLSLVYSMTLVLLFSVLRPPAALSDPLRIVLPSVAYDVVVAAILGPLIVSIRDRFT
                     DEERAPR"
     gene            22330..24252
                     /gene="mrdA"
                     /locus_tag="VHM48_06920"
     CDS             22330..24252
                     /gene="mrdA"
                     /locus_tag="VHM48_06920"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2755177.1"
                     /translation="MNPYLDGQPIAKRPGSRFLVFGIAAILAISGLTTRLFYLQIVSG
                     GQFAALTQVNREVEQAIPSSRGLIFDRAGRVLVKNVLTYAVKLRPVDLPEERRDEVVA
                     RLSALLKMNIADINGEIDGNPGSRFDLVPIASDVPESTANLISESGDALPGVEVVTEA
                     RRQYPEGKLVSQLLGYTGPVSADQLDTLKKQGYLADDLLGKTGLEAYYESNLRGLYGS
                     ETVERDAAGRKLQVLERTREPQDGDSLKLTIDVKEQQYAQKALQWGMRAAGLKRGVVI
                     VMNPQTGEVLAMVSLPTYDDNAFAKGISSKDFSRLLNDKNKPLLNHAIQAHYPPGSTY
                     KIVAGSGALADNKISATSLVHTRGFLILGRTKFWEWNHRGWGAIPIKLGFAHSSDTFF
                     FQLAGMLGIERLAHWAHQYGFGAPTGIDLPGEVAGIVPTNAWKQEHFGEPIYPGETYQ
                     AGIGQGYDVVTPIQLINAYAALANGGKLYSPQLVREIVGPDGSVVRRFTPKLIRKLAV
                     PRSDLKTMREAARNVLLVRHTYNLVDLPIVIGGKSGTAEFGLRDSKGRLPFHSWFVAF
                     VPKHAAKTASDPTGMKAIAREDSNLIVLAFAYDSRTKGNAATEIVKYYLQLHFHIKHD
                     YRNFYLLQRGNFYQSN"
     gene            24299..25459
                     /gene="rodA"
                     /locus_tag="VHM48_06925"
     CDS             24299..25459
                     /gene="rodA"
                     /locus_tag="VHM48_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX2755178.1"
                     /translation="MGVIRTEPARMTDWAARSIGAAWRAFDLQLAAYAALLAILGLVM
                     AYTNSVEQGQAVLSGGTTFVRGLMWTGIALIAFVVTTAFDYKWLKTFAWPLYLLQIVL
                     LIATLAVGHGVGGSSRWISFGPFDFQFSELAKILMIVVLANYLGARQGRLRSLGSILG
                     ACILVGPPFALVMLQPDLGSSLVLLAILAGMLFMSGARMLWLTILTVVGMVPLPLIWQ
                     YVLHDYQKQRLTAFFNPASDTQGAGFQLFQSQIAVGSGGLLGKGLTNGSQNQASLLPV
                     QESDFVAAIYLEELGFIGAMILLILFGALIWRLLVSGWRSKDPFGMMFASGLASMILF
                     QLVVNLGMVIGIMPITGIPLPFISHGGASLISLAVGLGIVQSINIRQTRAEW"
     gene            25530..26717
                     /locus_tag="VHM48_06930"
     CDS             25530..26717
                     /locus_tag="VHM48_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2755179.1"
                     /translation="MWLLVALLAAAVAVAIVTRRSALPYTVGLVLFGLTLSLIGPAVA
                     FPVSPKLLLAIVLPGLVFEAAFRTDLAILRRSAGWIFLLAIPGVVVVATIVAAILSAT
                     TTLTFTESFLVGAMVAATDPAAILGTFRQIRVPIRLETIVEMESLVNDGTGIVLFAVA
                     LEMLEGGGSIGGSVVVFAVSLVGSAILGALLGAVAARIVRGIDEHLTQLTITVVLAYG
                     TYLAADAVGLSGVITTLVAAGAFGTIARGDLTSQTRDTIDVVWEFAAFLLTAGVFLLV
                     GLAIAPSTLALAAEPIVWGVLAILIARAIVVYGLLGIPATLFARRGRQDLAIPRPWLH
                     VLFWAGLRGAVSVALALSIPSDVPNRELIQGVTFGIVLFTLIVQGSTAGLVVRRTGAS
                     TTS"
     gene            complement(26764..27474)
                     /locus_tag="VHM48_06935"
     CDS             complement(26764..27474)
                     /locus_tag="VHM48_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2755180.1"
                     /translation="MTDGPDPYKVLQVDSEAEDEVIQAAYRRLAQKYHPDVATGPDAD
                     ARMAAINAAWEILRDPAKRAAHDLARRLAARNAERAAAAGRSAAHQPAHASSRSPAPG
                     TSGSDRGTSPGPAADPPRRADPPRRAETVSSDWTSGRSSQGGGYDPARMGAPDGLGAA
                     GPPPGDPSGSVLNFGRFAGWSIGEIARRDLDYIEWLDRMPIGRPYRDELDVILRRSGR
                     RKSASAEAQERRGLFRRR"
     gene            complement(27479..28660)
                     /locus_tag="VHM48_06940"
     CDS             complement(27479..28660)
                     /locus_tag="VHM48_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2755181.1"
                     /translation="MAPTDQARAGFGPLSPRVVLVLAGSAVVAVVLHAGSSALGPFVV
                     GLVLAYLLDMPVERMSRIGLPRWLAVLIVYAVVVVLVVEGGVMAMRPLSDEFATFIRE
                     FPKFTAQVTDIYAHLDLLPAVRHAIDTWLADLGSGIAGIDPAAVLPVVSGIAGIVGSI
                     VGYVLIPVWIFYLIKDRPALTAAAERSMPDAWWPDVSAVSSLVLRVFGQWVRGQLVLG
                     VTVGAATFAGLLLLSVTVDPIFGRFAILLSVVAGVLELLPIIGPIIAAIPAVLLALTA
                     GLDVAVAAVILYTGIQQLENYFLVPKIQGDAVELHPSAVMFALVIGGAIAGLLGAILA
                     LPISAAARDVFRYLFHRFDDPPATPDEAVARIRARPTIVQPVPEPAADAADAEDAELE
                     A"
BASE COUNT         4230 a         9634 c        10055 g         4818 t
ORIGIN      
        1 cctgtcgatc cggcgctgaa gccgatcgat cgagtaggcg ccgtcgatga aggtctcgat
       61 tgtcgaagcc gacagcaagc cggcataggt gtcacgccat gccgcttcag ccacgtctcg
      121 cacggaggcg gcgtcgtcca cgtccgcatc acgcacggtc aacggcacgg ttcggtcggt
      181 ccagtcagcg tccgcgctgc gcatcggcca tctcgcgggc gatatcgcgc ttggcgtcgc
      241 ggtcggcgat gtcgcggcgc cggtcgtaca ggttcttgcc gcgcgccaga ccgagctcga
      301 gcttggcccg gcccttgccg gtcaggtaca gccggagcgg gacgatcgtc tggcccttgg
      361 ccttcgtccg gccgagcagc tcgtcgatct ccgagcggtg gaggagcagc ttccggatcc
      421 gcttcggctc gtggttgtag cggttcgcct gctcgaacgg ggcgatgtgg gcgccgatca
      481 gccacgcctc atcgcgctcg atcctggcgt atgcgtcgga caggttgacc ttgccggccc
      541 ggatcgactt gatctccgtc ccggtcagga cgatgcccgc ctcgaacgtc tcgtcgatgg
      601 tgaagtcgtg acgcgcccgc cgattgaggg cgatcgtctt ctcgtcagcc acgtgggttc
      661 cttgtggtca cgccgccttg ggcgcagaag tgtcgttcat ttcggccgtg cccgcgactg
      721 cgttcgacag ccgaacgtct cgccccagag cgcaggaaag ccggccctag ggccggcttc
      781 cttagcgttc ccacccacag gggtgaccga ctagacggcc atctcctgcg aggtggtgca
      841 gctacgcgta ctgtcgatca ctgcacatca cctcctttcg ttccgcgcca ccgtacagga
      901 accgcgggca gcgtgcaagc actccgcgaa cgcgcagatt cgcagccgtt gcgcgtcgtc
      961 gccaggtgcc tcaggccgcc cgcgacaggg cgctcggaag ggatgcggac gccttcgtca
     1021 ggagctcgac ggccttgtcg aggaccggat cgttggtcgg tccggcgtcg gcgggcgtcg
     1081 cgaccggaac gtccgggatg atcccgacat ggtggatcca gtgcttgtcg ggggtcagcc
     1141 acttggcgat cgtcagcttc agcgccgagc cgttctggag ctggatccac tgctggacgg
     1201 tgcccttgcc gaatgatgtc tcgccgacga tcgtcgcccg cttccggtcc tggagagcac
     1261 ccgccacaat ctcgctggcc gaggcggagc ccttgtcgac gaggacgatg agcttgatcg
     1321 acgcgtcggt cgcgatcccc tggccggtcg aatcggtctc gacctgggcg ccgtcggcgt
     1381 cctgctccca gaagatcggg ccgtccttga tgaactcgct cgcgatcgac cgcgccgccg
     1441 tgacataccc gcccgggtcg ccgcgcaggt cgaggatgat cgccttcttg ccggccttga
     1501 ggtcggcctg cagggcgtcg tggaacttcg tctgcgagtt gtcggagaag ccggtgaccg
     1561 cgatgtaccc gatggtccct tcggcgaggt ccctggcgat gacctccttg gagacgatga
     1621 cgtcgcggac aaccttgatg tcgatcggcg ccgccttgtc acggacgatc gagaggacga
     1681 cctcggtccc cttcttgccg cggatcttgt tgcgggcgcc gtcgacggtc aggccgtcga
     1741 gcgacgtgcc gtcgaccgcc acgatcgtgt cgccggcctt gaccccggcc ttctccgacg
     1801 gcgagccttc gatcggggtg accaccacga gctggcagtc gggtccgagg gtcgtgcagt
     1861 cagacgacac acccttcgcg tcatgcgtcc cgatctcggc cccgatccct tcgaactggc
     1921 ccgacaggtc ctgcagcccc tggctgtact ggtcgggggt caggtaggcc gagtacgggt
     1981 cgccgagcga gtcgaccatg cccttgatcg cgccgtcgat caggctctgc tgggtctcgc
     2041 cgccgagggc gaaccgcttg gtgatcgtgt cgtagctgtc ccagaacggc tggaacgcct
     2101 gctccgaggc gacgggcgtg cccggctgct cgatgctccg ctggccgacg aggaagccgg
     2161 acacgaagag cgcgccgccg gcgaggacgg agaccacgac catggcgatc gccaggatcg
     2221 gcccgcgcac gctcgaggag ggacggccgc cggccggagc cggaacgggc tgggtcggca
     2281 ccgcgtcgat cgggccggcg ccggcaggat ccgcgtgatc cgcgtcgaga gcgtccgatg
     2341 gcgggccgac cgccggatcg gtggtgagcc cgggatcggg cacggaggag tcggaaggga
     2401 gcatcgagga cgtggttctc cgctttgatg agccggcggg cttgacaggt tacacccgcg
     2461 gttgagcgat gctacgttcg gtggacggcc gtcggatggt ccgtgaacgg aacttcacgc
     2521 aaatgtggtg cgccgttacc ggatgagata ggtccggacc gaaagccacg acccgagcac
     2581 gccgagaccg agaccagccg acgtcacgat gaccacgagg tcgcgcgcca gcgagccgaa
     2641 gctcagtggc aggacccgga agaagtcgac catgaagccg ccgatcgggt cggccaggaa
     2701 gtacagcccg atcagggtga tcgcggcccc gaacagcccg accatggcgc cctcgaacac
     2761 gaatggccag cggatgaatg cgtcggacgc gccgacgagc cgcatgatct cgatctcttc
     2821 cgcgcgcgcg accaccgcga ggcggatcgt gttcacgatg atgaacagca cgatcacgcc
     2881 gatcacgacg aggatcacgc tgccggccgt ccgcatgatg tcggtgaccg tcttgacgtt
     2941 ctttgccgtg tccttcgtgt cgaggacgtt cctgactgcg gggttgaggt tcggctgcag
     3001 cgcggccgcg accgggctgg cgaagctcgg gtcggtcagc ttgatctcgt agctcggcgg
     3061 gaacgggttc gagtcgagga ccttggagag gtcctcgcga ccctggttct tcatgctctg
     3121 ctggaagcgc gcgagggcct cctcgcgggt gaccagggtg gcccccgcga ctccgggcaa
     3181 tgccttgatc gtcgtggcca ggttcgtgat gtcgacctgg ctggcgtcga ccttcatgtt
     3241 cacctggacc tcgaccttcg actcggtgaa atcgagggcc gacagcaggc cggtctggat
     3301 gatccagaag ccggacagca ggaggagcat caggaccatc gtcgccgtcg cggcgaggga
     3361 catcagggcg ttccgccaga atccctgcca ggcgcggcgg aggctgaaca cgacgaagcc
     3421 gatcatcggc tgccgctcga caggcgcggg cggcaagggc gacaggctgg gttcggcgtc
     3481 atcagcggct caatccgaac ggtggtaggc gccgccgacc tcgtcgcgga tcacccggcc
     3541 ctcctcgagg gcgacgacgc gcttgcggag ggcagtgacg acgtcggcgt cgtgggtcgc
     3601 catgaggacc gtgaccccga gctcgttgat ccgcaggagc agctggatga tctcccagct
     3661 gatcagcggg tcgaggttgc cggtcggttc gtcggcgatg atcagccgtg ggtcgtggac
     3721 gagcgcccgc gcgatcgccg tccgctgctg ctcgccgccc gacagctggc tcgggatctg
     3781 gttgcgctga gcggtcagcc cgacgagggc gaggacccga tcgaccgcgg gccggatctt
     3841 gcggcgcggt gagcccgtga cctcgagggc gaacgcgacg ttctcccaga cgttcttggt
     3901 cggcaggagc ttgaagtcct ggaagacgat cccgatcttg cgccggacct tcgggaccgc
     3961 ccgccgcggc agcttcgcga ggtcgtggcc gtcgaggatc acctggccct gggtcgcgat
     4021 ctcgtcgcgg atcagcagct tgatgatcgt cgacttgccg gcgccgctcg gtccgacgag
     4081 gaagacgaag tcgccttccg ggatcacgag atcgacatcg cgaagtgccg tcttcccgtt
     4141 gggataggtc ttcgtgacgt cgtgcagcac gaggacggtg cggtcgaccg cggaccgatc
     4201 gcgaccgcga cggaggagtc gctcgcgcac ggagcgcgtc gtggcggtcg ggacgggggc
     4261 gagggtcaag agcggctcca cggtctggca gtcggctggc cggcgcgatg gtcggccagc
     4321 gggactccgt ccgggggcat gtcaggggga gccacgtcga tcccgaggct accacctcgg
     4381 gccaatcgcg tcgcgcgcgg ccgccgtctc agagcagccc gggcgacatc gggatgtagc
     4441 gggagaagtc ggcgaacggg cgatcccagc acagcagcac cgggaagccg tcgaaccgga
     4501 agccggcgcg gaggagcggg acgaccgctt cacccgccgt gcccggaacc cacaggccga
     4561 aggcgccgcg tggcctgact tcgtggacga gatgtccgac gaccggtccg agcagagccg
     4621 cctcgcggac ggcgatcggc ccgacacgac cggcctccga gctgtacccg taggcgacgg
     4681 cccgtccccc ccggtcccgg aagaggaaac cgattcggcc ttctcgggca tggaaccggt
     4741 gatccgcagt ccggctgaac ccggcggcgt cgcggtccag agcgtcgagc tcggcggcca
     4801 gggcggatcg accgaggccg tccccggcgt cgccgacctc cgcgaaggcg atcgcctcga
     4861 cgccggccgg cagcggcccg aacccgccgt cgcgcgtcgg caggccgacg agccggagga
     4921 gcgggatccg ggggaccatc ccgagcgagg cgtacagccc gttcgagatc ggctgggcgg
     4981 tgtcggtcga ggtcgcccgc gtcaccgacg cgctcgaatc gctcgaccct tcaggggcga
     5041 ccgccgccag gagcgcccgg cccagcccgc gtgcctgggc ggccggccgg acgaagagca
     5101 tcgagaggta ccacaggtcg tcgcgccgga cgacgacgac gaatgcgtcg atcagggcgg
     5161 gctcgccggt cccgtcgcca gacggccggg cttccggcgc cgcggcgacg aggaacgtgg
     5221 tcgggtcggt cgcatggagg tggccgtaca gccgcaggag tgacgcgagg tcgtccggga
     5281 tctccggctg ggcgagccga ccgaggtaat cgttcagcga ttcccgccag atcgcggcac
     5341 aggtcgggag gtcatcgggt gtcgcccgcc gaatgcgatg gcccgacgtg gtgaggatct
     5401 ccgtgccacc gcgtgggccg gcggtcattc ctcgtcggcc tcgtccggcg ggccgccggc
     5461 gagaccgccg cccgtcgcca gtcgctccag ctcggcctgc atgaacgcat cgagatcgcc
     5521 gtcgaggacc gcctgggtgt tgccggtctc gtgggccgtc cggaggtcct tgaccatctg
     5581 gtacgggtgg aggacgtagc ttcggatctg gttcccccag ccggcctcga catgctcgcc
     5641 cttgagcttg cgaacctccg cctcctgctc ctcgagggcc cgctcgagaa gacgcgcctt
     5701 cagcacccgg atcgccatct ccttgttctg ggtctggctc cgctcgttct ggctctgggc
     5761 gacgatactc gtcgggaggt gggtcagccg gaccgcggag tcggtcttgt tgacatgctg
     5821 gccgccggcc ccctgggagc ggaaggtgtc gacccggatc tcgtcccagt tgagctcgat
     5881 ctcgatgtcg tcgtcggctt ccggcaggac ctcgaccagg gcgaacgtcg tctggcgccg
     5941 tttctgcgag tcgaacgggc tgatccggac gagacggtgg acgccgcgct cggcccgcat
     6001 ccagccatag gcggcccgcc cgtcgatctc gaccgtggcg ctcttgatgc cggcctgctc
     6061 gccctcctgc tggtcgacga tctcggtctt gaaccggtgg cgctccgccc agcggaggta
     6121 catccggagg agcatctccg cccagtcggt cgcctcggtc ccgccggccc cggcgctgat
     6181 cgtgaggatc gccccaccct ggtcgtactc accggagaag aggagggcgg tccgctcgcg
     6241 accgaagtcc gcctcgagcg cggcgacctc ccgatcgagc tcggcggtca gctcggcatc
     6301 gccggattcc tcgacaagct cgagggtcgt caacaggtcg tcggcgcggc cctcgaagcc
     6361 gcgccacagc tcgacgtcgg agcggaggcc gttcgcctcg cggaggacct tctgggcgga
     6421 ccgctggtcg tcccagaagc cgggctcgct ggtcagcgcc tcgagctgcg agacgcggcg
     6481 ttccttgacc tcgaggtcaa agatgccccg aggtcgaccg aatccgctcg accaggtcgc
     6541 gaaccgtgga ggcgtccact cagttgcgag cctggaggtc ctggagcagg atcggacgga
     6601 gctcgatcag acgcagggcc gacttgctcc gggcgacgac ggggttcacg cagggacagt
     6661 agctgttgtg agggcagacg ccgcagttgc cgtcacagtc gagggccgtc ccgtaactgc
     6721 acgagcggca gtcacgttca caggcaatga gatcgacgaa tgacgcttcc tcggcctgct
     6781 tcaactcgtc cctctctctg aactggcgca gggttggggg cgcggcgcat gacgtggctc
     6841 aggggattct gaccgatcca ggggcctgcg cggattgggc ggagtatagg gccttggtct
     6901 cctccggcca agagggagaa cccggaagtt tcgcggttcg agccgatttg gccatcaccc
     6961 gggggggccg aatggaggcc cgatcaacga cgtcgcgaat ccacgacgcc cgggactcag
     7021 ggcgtctcgc cggtgacgaa gtacagcgtc agcccgccga gctcgtgggc cgtcgatcgg
     7081 agccgatcgt cgaaggtcag gtcgctcggg ctcgtcgccg tcggtgagcc gtacgccgtg
     7141 atcccctcgt cgcgggcgat gcgcaggact cgcagcatgt gggtccggtc ggacacgaag
     7201 atcgcggtct gcagaccgcg aacgtgaatg atcgtggccg cgccgcgaag cgattcgagc
     7261 gtggttcggc cacgatcctc ggcgaggatc gcggcgtccg gcacgccgcg accgatcgcg
     7321 taggcgcggg cggtggccgc ctcggtcgtg atgtcgaggt cgagctttcc gccggtcacg
     7381 atcaggtagc gcgccacgcc gtctcggtac agatcgatgg cgtggtcgag acgagccttc
     7441 aggatcaccg atggccggcc gttgtactgg gcagcgccga ggacgacgat cgcatcggcc
     7501 ctcggccggt cgtcgtgatt gccgacgtcc cagatccgat agctcgccca gccggcgacg
     7561 agcacggcgg cgacgatacc ggcgaggagc agtcggccga ggttccggat catgccggcc
     7621 gcgacggccg gagccccggc agcggatcgc gcgggaccgc gaccagtcgg acgccgtcga
     7681 gcgggacgtt gacgttgacg agtcgggcgc cgtcgagcgg gacgtcagcg tcggtgggcc
     7741 gccggttcag cgcccgtgac acttcttgta cttctgaccg gagccgcacc aacacggatc
     7801 gttccgtccg atgcgggcgc ccgtcggggt gaatccgggc ttcgcctggc cggccggcac
     7861 gctccgtccg ttgccgctcg tctcgaggcg ggcggccgcc atgggatcgc gcggcaggcc
     7921 gcccgccacc accgggccga cgggcctggg accggccggg cgtccaccgc ccgcggacgt
     7981 cgcgctggag ccgctcgtcg aggtcgccgc gccaggccgc gaacccccgc tcgcggccgc
     8041 ggtgagggcg cgggcgccgg cggcgaggct ctggtcgagg tccgactcgc caggcggcgg
     8101 cgtccgggtc acggtgaccc ggaagatcgt cgcggcgacc tggtggcgga tgaggccgcg
     8161 cagctcgccg tagagctcga acgcctcccg gcggaactcg ttgaggggat cctgctgggc
     8221 gtagccgcgg aggccgatgc cgcgccgcat gtcgtcgatc tcggtcaggt gctcgaccca
     8281 cagggagtcg atcgtccgga gcaggacgtg gcgctcgaca aggctccagt cggcctcgcc
     8341 gatctccgcg gcgcgggcct cgagccgggc ctcggccatg tcgagcaggt gctcgaggat
     8401 ctcctcgcgg ctgccgatct cccagagctc gtcctcggat gtcccggggc cgtcgagacc
     8461 catcgccttg agcgccgcag cgagcgcctc catgccccag tcgtcgggcg tgtcgcccga
     8521 caggtggtcg gccacgagcg ttcggacgtc ctcgtcgagg aacgtccgga ccgtctcgcc
     8581 gaggtcctcg ttgcgcagca ccttgtcgcg ttcggcatag atcgtctcgc gttggcggtt
     8641 gatcacgtcg tcgaactcga cgacgcgctt gcgcatgtcg aagttgaagc cctcgacccg
     8701 gctctgggcg ctctcgatcg tcttgctgac gagacggctc tcgatcgcga cgtcgtcctc
     8761 gaggcccatc cgctccatca gcccggcgac gcggtccgag gcgaaccgct tcatcaggtc
     8821 gtcctcgagg gacaggtaga agcgcgacga gccgggatcg ccctggcggc cggctcggcc
     8881 gcggagctgg ttgtcaatcc ggcggctgtc gtgacgctcg gtgccgatga tgtgcaggcc
     8941 gccgacgccg accacgcgct cgtggtcctc gtcggtggtc gtcctcgcct cggcgagggc
     9001 cgcggcgtag gtctccttgt cgacctcggc cgggttcaag ccgcgcttgt ggaggttctc
     9061 cgaggcaaga cccgccgggt tgccgccgag gaggatgtcc gtgccgcgcc cagccatgtt
     9121 ggtcgcaatc gtgatcgccc cggtccggcc ggcctgggcg acgatccccg attccttctc
     9181 gtggaacttc gcattgaggg tctcgtgctt gatccctcgc cgctggagca tcgtcgagag
     9241 gacctcggac ttctcgacgc tgaccgtgcc gacgaggacg ggccggccgg cctcgttcat
     9301 ctcggcgatc tcgtcgatca gggcattgaa cttgccgagc tcgttgcgga acaccagatc
     9361 cgcctcgtcg tcgcggatca tgtccttgtg ggtcgggatc gagacgacct cgagctcgta
     9421 gatcttgtgg aactcctcgg cctcggtcat cgccgtgccg gtcatgccgg cgagcttgtc
     9481 gtagaggcgg aagtagttct ggaaggtgat cgtggccatc gtcacgctct cgcgctggac
     9541 cttgacccct tccttggcct cgaccgcctg gtggagacct tccgaccagc gccggcccgg
     9601 catctggcga ccggtgaact cgtcgacgat gatgatctcg ccgtccttga cgatgtagtc
     9661 gcgatcgcgc cggtagaggg cgtgggcgcg cagggcctgc tcgaagtggc gggccagacg
     9721 cggatcggcg tcgtacaggt tctcgacgcc gacgagcttc tcgatcttgt cgatcccctc
     9781 ctcggtcggc gagaccgcct tgtccttgag gtcgacgaag tagtcgccgc cctcctcctc
     9841 gccctcccgc cggcccgtga gcttcgggac gagacgggcg aacgtgtagt agagatcgcc
     9901 cgactcctcg gcctggccgc tgatgatcag cggggtccgc gcctcgtcga tcaggatgtt
     9961 gtcgacctcg tcgacgatcg cgaacgagcg ttcgccctgg acccggtcgg cgaggtccgt
    10021 gaccatgttg tcccggagat agtcgaagcc gaactcgttg ttcgtgccgt aggtcacgtc
    10081 ggccgcgtag gcatcgcggc ggctgaccgg gcggaggttg atcaggcgtt cgtcgctcgt
    10141 cgggtagtcc ggctcgaaga cgaacgactc gtcgtgggtg atcatcccga ggctgatgcc
    10201 gaggaagtgg aagaccgggc ccatccactg cgggtcacgc cgggcgaggt agtcgttcgg
    10261 ggtgacgacg tggacgccgc ggccggtcag cgagttgagg gccgccgcca gggtcgggac
    10321 gaaggtcttg ccctcgcccg tcttcatctc cgagatcttt ccctggtgga ggaccatgcc
    10381 gcccatcagc tggacgtcga aatgacgcat ccccagtgtc cgcttcatcg cctcgcgggc
    10441 catcgcgaag acgtccggga ggacctcgtc gagggccgcc tggagcgctg cctgctcccg
    10501 cttgtggcgg tccttcttca tctcgcggcg gcgttcgagg tcggggtggg tgagctcctc
    10561 ctcggtcggc tcgcccggca cggcggcctc gtggatctcc tcgcggatca ggccgaaccg
    10621 ctcgcggatc gcctcgtcgg agagggcggc aatttcggtt tcgagttcgt tgatctcgtc
    10681 gaccaggggc tggatacgtc ggatctcgcg gtcgttcgag tcgacgaaac gggaaagaaa
    10741 tcgcatggcc gatgaagtat agggggagtg cctcccgggc ccggccgccg tgtcggtgcc
    10801 tcgcgggcag tcgaccggca caaggcccgc gccccagctg ttcatcagac gcacgaatcg
    10861 ggatcgagcg tgcattggat gaaccgatgc gacaggtcag gcgacgcctt ggacgcgggt
    10921 cggtccgatt cccggcccgc gcgacgggat cggtcgagtc tgctcatcac gtgcgccagg
    10981 gacgacacct ctcgagtcaa cgcggcatcc cgtcgtcgca taggttcact ggatgcacgg
    11041 ggcggccccg cgctgcgctc ggacgccggg gcaggccgtt gtcggatctc cgccggtcag
    11101 gcgagcgtcg actggcctct cggtcgggcg cggcaaatcc ccgccgcgca tgcgagcatc
    11161 gcctacgcga tgcacccctc acctcttgct cgtgcatgac gcgccggggc gcccggaagc
    11221 cagcgacgcg caccgcgccg cgctcgccgg gacgcaagaa ccgggcaggc aggcggggct
    11281 cgcgacggtc tcagctcttc gccaggttca ccaggaattc gagcgtgcca tggtcctcga
    11341 ccgcgacgaa gccgccgatg ttcggggcgt tgatcgcgaa gtcggagaac gggaagtcga
    11401 gcgagccgac gacctggatc aggctcccgt tgagctgcgc cttggccggg atgtcgaccg
    11461 tcttcgtcac gccgtggagg gtcaggtcgc cgtggagcgt gacgctgacc tgggcgcccg
    11521 tcgtcgcggc ggcgggcagt gcgaccgggg tcgtgagctt gaacgtcgag gtcgggaagc
    11581 tgtcggtctg gatcccgtcg ttgcgcaggc ggttgtcgcg catctggcga tcgctcttga
    11641 tcgtggtcat gtcgacggag aaatcggcgg cggcgacgct cagcgtgccg ccgctcgcgg
    11701 cgatggtcgc cgttccggtg atcgtgctgg tgcggccgac cgcgtcgctg tcggccgaca
    11761 ggctggcgag ccgctcgcgg acccggtagc cgacgaccga gtcggcgccg atcgtccagg
    11821 ttcccggcat gcttgccgcg tcgacggccg ccgcggatgc gggcgggagc gaggtcgtgg
    11881 cgccggacgc acccgccgcg gggctggcgg cggccgatcc ggacgacgag gtgggcgccc
    11941 ggctcggcag gctgagcggt gcgacgttgt cgccacgaag gaagagggcc caggtggcgg
    12001 caccaccacc gacggccagc aggaggatga cgacgatggc gagcagggcg ggtcgggatc
    12061 tcatggcccg atcgttcgga acgccggtga ggggtttctg agagtcaccg aggcacatcc
    12121 tcgaccgatg gcttcgcgga gctcggcctc ggacctcgga gagctcagtg ccagaaccca
    12181 atcccgtagc cggccacaag gatcacgaac gtcagcgcgc cgaggccgac ccagaagatg
    12241 acggccatcg cggtcgaggt cgggttcatc tccgggttct tcatctcggg cagttgcccg
    12301 tcgcgccggt tcaaccccca gcgacgagac agcatgcccc gccgccagtc gggttgactg
    12361 accgggcgcg gctcgacgcc gcccttgccg tccggtcggt ggagctggag gctcatcgat
    12421 ctccctgtcc tgtcgcgccg aggaggactg gatcatcctc cgtgtcgtcg tccgcgcccg
    12481 ggtcggatcc gtggtcgggc tcctggcgtg tgtccatccg gcgctgctgg ccgtcctccc
    12541 agaggagcat ctcctgggtg aacgagacct cgctgctgaa tagagcgccg accgactcgc
    12601 cgcgatggat ccgcttgatc gcctcgccga tcagcggagc gaccgacagg gtcgtgacct
    12661 tcggcagccg cttgatcgcg ggcagcggga ccgagtcggt caggacgacc tcgagcaggt
    12721 tggagtcgcg gatccggtcg accgccgggt cgctcaggac cccgtgggtg gcgcaggcgt
    12781 agatctcggt gacaccctcg cgctcgagcg cccggacgat ctcggtcagc gttccgccgg
    12841 tgtcgatctc gtcgtcgacg atgatcgccc gcttgccgcg gacgtcgccg atgacgttca
    12901 tcagctcggc ccggtcgagg ttcccgatcc gccgcttctc gatgatcgcg aggggcgcgt
    12961 cgagcagctc cgcgaacgtc cgcgcccgct tggcgaagcc gaggtcggtg acgacgacca
    13021 cgtcggtcag gtgcttgtgg atgaagtagt tcgagaggag atggacggcg gtcagctcat
    13081 cgaccgggat gttgaagaag ccctggatct ggccctggtg gaggtccatc gtgaggaccc
    13141 gatcggcacc ggcgacggtg atcatgtcgg cgatcaggcg agccgtgatc gggacgcgcg
    13201 gctggtcctt cttgtccgac cggccgtagg cgtagaacgg aatgaccgcg gtgatccgcc
    13261 cggcgctcgc ccgtttgaag gcgtcgatca tgatcagcag ttccatgatc gactggttga
    13321 ccgggtgcga tgtcggctgg acgaggaaga cgtccttctc gcggacgttg tcgaggatct
    13381 tgacgaagat gttctcgttc gcgaactgga agacctcggc cttgccgcac tccgtgccga
    13441 gccagcgggc gatcttccgg gccagttccg ggttcgagtt gccgtgatag atctcgaact
    13501 ggtccgcgta caccgcgtcc tcccgaacga gcggtctcac gacgccgtca cgcgtcctgc
    13561 cccggaaccg gcccgctgcc gcccggttct gggccgccat tgtcctcgat gccatcgcgc
    13621 tccgccagcc cggaccggaa cgccgcgatc gccacgaccc gatcgccctc ggcgaggcgc
    13681 atgacgatca cgccgcgggc acccgacgag tagcggttga tggagttcgt ctcggtccgg
    13741 atcacctggc cgccggcgct gatcaggagc agctcctcgt cctcctcggt gacctgctgg
    13801 accgccgcga cgagacccgt cttcctgcct tcgagggcga tcagccggac gccctggccg
    13861 ccccgatgct tcttgcggaa ctcggtcagc ccgacgcgct tcccgtagcc ggtctcggac
    13921 aggacgagga ggtcggcacc cggctcgacg accgtcatcg cgaccacgct gtcgccttcc
    13981 ttcttgagga ggcggatccc gatgacgccg gccgcgtcgc gacccatcgg ccggacctcg
    14041 tcctcgtgga accgggcgag catgccctgg gcggtcgcga ggatcacgtc gtcggccccg
    14101 ctcgagacgt cgacccaggc gagctcgtcg ttgtcgtgga tcgagaccgc ccggatgccg
    14161 gtcgagcgga cccgttcgaa ctgctcgagc ggcgtcttct tgatgtggcc gcggcgggtc
    14221 gccatgacga ggtagtggcc cggctcgaag ttcggcagga cgatcgtcgc gaccggcacc
    14281 tcgccggcct cgacctggac gcccggcagg ttgatgatcg gcgtgccctt ggcctggcgg
    14341 ctggcgtcga cgatcgtgtg gaccttggcg ctgaacaccc ggccgcggtt ggtgaagaac
    14401 agcgcccagt cgtgggtgtt ggccacgatc aggtactcga ccgcatcctc ctcgcgggtg
    14461 acgtggccga tgatcccctt gccgccccga tgctggcggc gataggtcgc gaccggctgg
    14521 cgtttgatgt aaccgcggcc gctgatcgtg acgacgacgt cctcgtcggc gatcaggtcc
    14581 tcgtcggtca tctcgcggct ggtgtcgtcg gcgacgcgcg tccgccggtc gccgccgaac
    14641 ttcttcttca gctcggcgag ctcgtccttg atgatgccga ggacgcgggc gggattggcg
    14701 aggatgtcct ccaactcggc gatgagctgg atgacgttga ggtactcgtc ctcgatcttc
    14761 ttgcgctcga gggcggcgag gcgggccagc cgcatgtcga ggatcgcctg ggcctggagc
    14821 tcgctcaggt cgaagcgact catcaggttc gtccgcgcca gctccacgtc ggccgactcg
    14881 cggatcgtcc ggatcaccgc gtcgaggttg tcgagggcga tcttgaggcc ctcgaggatg
    14941 tgggcccgcg cccgggcctt gccgaggtcg aactcggtcc gccggcggac gatgttgcga
    15001 cggtggtcga cgtagtggac gaggacgctc ttgagcggga gcgtctgggg ttggccgtcg
    15061 accagggcca gcatgttcat gttgaacgcc agctgcatcg gcgtgtgctt gaacaggttg
    15121 ttcagcacct tgtgcgggtt ggcgtcgcgc ttgatctcga tgtagacgcg catcccgtcg
    15181 cgatcggatt cgtcgcggag gtcgctgatc ccgtcgatct tcttgtcctt gacgaggtcg
    15241 gcgatcttct cgagcagggt cgccttgttc acctggtagg gcagctcgct gatgatgatc
    15301 gccatccggt cgccgcgaac ttcctcgaac gcgacctggg cgcgcatgac gacccggccg
    15361 cggccatggg catacatgtg gcggatcgcg tcgacggtct cgttctcacc ggtcagcggg
    15421 ttccgctgct gctcgaagcg atagatcgtg ccgccggtcg ggaagtccgg cccgaggacg
    15481 tacctcgaga ggtcgtccga ggtgatgtcg gggtcgtcga tcagggcgac ggtggcgtcg
    15541 cagatctcgt tgaggttgtg aggcgggatg ttcgtcgcca tgccgacggc gatgccgctg
    15601 ctgccgttga tcaggaggtt cggcagcttc gccgggagaa cggacggctc cttctgggtg
    15661 ccgtcgtagt tgtcctggta gtcgaccgtc tccttgtcga tatcgtcgag catctcggcg
    15721 gcgatgccgg tcaggcgcgc ctccgtgtag cgcatcgccg ccgcgctgtc gccgtcgacc
    15781 gagccgaagt tgccctggcc gtcgacgagc gggtagcgca tcgagaagtc ctgggcgagg
    15841 cggacgagcg cgtcgtagag ggcgacgtcg ccgtgcgggt ggtacttgcc catgacctcg
    15901 ccgacgatcg cggcgcattt ccggaaggag ctcgtcgagc tgagccccat ctcgcccatc
    15961 gtgtagagaa tgcggcgatg gacgggcttc aggccgtcac ggacatccgg cagcgcgcgc
    16021 gccacgatca cgctcatcgc gtagtcgagg tagctcaccc gcatctcgtc ctcgatgcgg
    16081 atggcgcgga tgtcgccgag atcgtcggtc acgggtcaga ctctcctaac gaacgttcgt
    16141 gtgggcggtc ggttgctgca tcctgggact catgccagcg gcggctcggg cagcggccgt
    16201 ccgagcaggc cggcaccgaa ccgctgggcc gtttgggagg cgcgcgacgt ggccggaccg
    16261 cccgcgcgtc gccggatgcc ggccaggcgt tcgcggatcc ggtcggcatc ggccggatcg
    16321 agcttcggca gggcgtcgcg gacgacccag gcgcggttgc cgtcagacga cgacgcggcc
    16381 aggtcggcct ctccgtcgag gaaggcggtg gtcgcggcgc gatcgatggc ggccatcgag
    16441 cggagggccc aggcgagagc cttctggacg tcgggcgcgg tgtcgccgat caactcccgg
    16501 acgaggccga gaccgtgcgt caccacctcg ggctggcggc cgcgccggcg gtcgacgaac
    16561 gggatcgtcg cgatcgtgct cccgacgagc cggcgctccc agcgcgatgg gctgtagacg
    16621 agctgctcga gctccgccca acggtacggc tcgttgagga tgcccttgcc gacgggatgg
    16681 gcgaggctgt cgaccgtgat ccagtcgccg gcgtcgcggg cggcgcgccg gaggagctgc
    16741 caggcgcgct cggtgtcgtc gacgatcagc cgctcgagaa gcccgaacgc aaaccagcgg
    16801 gcctcgagct gcggctcacg gaggaggcgg tcggcgatga acagccagct cgacgtccgc
    16861 tcccggcggg tcgcgtcgcg gaatccgcgc ttgaccgcct cgatcagcgg ccagcgaacg
    16921 ccgtggagat cgccgatccc gggtgcgatc cgctgggcgc cggcgaggta ctccgcgtcg
    16981 gccagcgccg cgaggccggc acgaaggacg tcggcgaagc gctcggggtc gcccagctcg
    17041 tcggcgaggg cccgaccgag cgcctcggca tccggccggc ggtcggcgac gaacaccacg
    17101 gcgcgcgccg tgatcgggct catggtcggc gccatcacgc ggggctcccg ccggccctcg
    17161 aaccgtcgct cgccggcgcc aatcggaacc ggtagcgtgc ctggagctcc gccttgcgcg
    17221 aagcatccgc ggccacatac gtcgcggtcg cggtcgcgag gacctcgccc gtggccggat
    17281 cgacgatccg ggcggtcgta tcgacgatgc gacggcggac cgccgtgatc cagccctcac
    17341 cgcggatggc gaccccgagg cggacgggcc gtttgaagtc gacgctcatc cgcgcggtca
    17401 ggccccagtt gtcggtggcc gcgagcgacc aggccatcac ctcgtcgagg atcgtgcaga
    17461 tgatcccgcc gtgggcgatg ccgtcccagc cctggaagcg gtccggcagg atcagctcgg
    17521 tccaacaacg atcgccatcg acatggaggt cgaggtggat cccgtgctcg ttgagggcgc
    17581 cgcaggcgaa gcagtggtgc ggggcaacct cgatcgtcag cccactgccg gggacggtca
    17641 cgacgcggcc ggcctcgtcc gtgacccgct caccgtccac cggttcgctg gtcgtggcta
    17701 tcgcccgtgc gtcgcgaacg tcagacgtca aggttccgga ccttgcgcgc ctcggtcttg
    17761 atgaagtcct tgcggggcgc gaccttctcg cccatgagca tcgtgaagat cgcgtcggcc
    17821 gtcgccgcgt cgtcgatctc ggcccgcagc agagtccgcg tctccgggtt catcgtcgtg
    17881 tcccagagct ggtcggcatt catctcgccg aggcccttga accgctggac ggacacgttc
    17941 ttgcggtcca tctccttgac gatccggtcg cgttccttct cggactgggc gtacttggtg
    18001 atcttcccgg tcgagactcg gtagagcggc ggctgggcga tatacaggta gccgttctcg
    18061 atgacctgcg gcatgaaccg gtagaagaac gtcagcagca gggtccggat gtgggcgcca
    18121 tcgacgtcgg cgtcggtcat gatgatgatc cggtggtagc gcagcttcgc gatgtcgaag
    18181 ctgtcgccga tcccctcgat cccggcgccg agggcgatga tcaacggccg gacgttctcg
    18241 ctcgcgacga tcttgtcgag gcgggccttc tcgacgttga ggagcttgcc gcggagcggc
    18301 aggatcgcct ggaagcgccg gtcacgaccc tgtttcgccg agccaccggc cgagtcgccc
    18361 tcgacgatgt agagctcgct ccgggccggg tcacgctcct ggcagtcggc cagcttgccg
    18421 ggcagggaca gcccatcgag cgcgcccttg cgaaggacga gatcacgggc cttccgggcg
    18481 gcatcacgag cccgcgcagc ggtgaggcac ttctcgatga ttcgtcgtcc gtccccggga
    18541 ttctcgtcga gatactgtcc gagcgcctcg gcgagggccg tctggacctg gcccttgacc
    18601 tcggcgttgc cgagcttggc cttggtctgg ccctcgaact gggggtcggt catcttgacg
    18661 ctgacgaccg ccgtcaggcc ttcgcggaca tcgtcaccgg acaggttggc gtcgttgtcc
    18721 ttgagcacgc tcgcccggcg ggcccagtcg ttcagcgacg tcgtgagcgc cgcccggaac
    18781 cctgtgacgt gcgtcccgcc gtcgaccgtg ttgatgttgt tcgcgaacgc gaggacgttc
    18841 tcggtgtacg tgtcgttgta ctggagggcg acctcgacgg cggtggatcc ttcccgcttc
    18901 tcgcagtaga tcggccgctg gtggaggacc tccttgttcc ggttcaggtg ccgaacgaac
    18961 gagatcagcc cgccttcaaa gtagaaggag cgctcgcgat cgacccgctc gtcgaccagg
    19021 gtgatccaga cgcccttcgt gagatatgcc gactcccgca ggcgctgcga gatgagctcg
    19081 aacgagtagt cggtcgtctc gaacatgtcg gcatccgcgc ggaacgccgt cgtggtgccg
    19141 cgccggccac cgctcgggcc gaccctgatc accgctgtct tcggtttccc gcgctcgtat
    19201 tcctgggacc agaccgtgcc atcgcgcgcc gattcgacgc gcatccacga cgacagggca
    19261 ttcacgacgc tcacgccgac gccgtgcaga ccgcccgaca ccttgtaccc gccgccgccg
    19321 aacttgcctc cggcgtggag caccgtgtgg acgacctcga gagcgtcctt gcccgtggag
    19381 tgcttgccga ccggaacacc gcggccgtcg tcgaccacga cgaccgtgcc gtccaccttg
    19441 acggtgacga ggatcgtcgt ggcatggccg gccatggcct cgtcgatcga gttgtcaacg
    19501 acctcccaga cgaggtggtg caggccccgg gcatcggttg agccgatgta catccccggc
    19561 cgacgtcgga cggcctcgag gccctcgagc acctggatgc tggaggcgtt gtactcggag
    19621 ccacccgcag ccttcgtccg cctcgaggtc ttccccgcgg tcgcggcttg cggctgggtg
    19681 gcttcttcgg tcacgcaggt cctccgatca aacggtcgta gagctgtccc agctcctcgt
    19741 cgctgtagaa ctcgatgatg atccggccac ccttccggga gcgggcgagc gtgaccttgg
    19801 tgccgagatg ccgtcgcagt tcgtcctcca cgcgttcgag atccgggtcc acggctcggc
    19861 gcgacgccgc cggcgtggcg gccacccgtg gttcacgaag gcggcgcacg agctcctcgg
    19921 tctggcggac cgagaaccct tgatcgacca cactcccgac gacccgcgcc tgttgctcgg
    19981 tcggcagtcc accgatcgcc cgggcatgtc ccgcgctcag ccgcccgtcc agcagggcct
    20041 cctggacgct gggttcgagg tccagcagtc gaatggtatt ggtgatcgtc gacttggcgc
    20101 ggccgacgac ctgggcgatc tggtcgtggg tcatcgcgaa ttcggaggca agcgagtgat
    20161 aggcgtgggc ctcctccatc ggcccgaggt ccgcccgctg gaggttctcg acgagcgcca
    20221 gctcgagctg gtcgcgatcg gcaagctggc ggatcaccgc tgggatgcgc tcgaggccgg
    20281 cctgctgact ggctcggaag cggcgctcgc cggcgacgag ctgatagccg tcgagcgttt
    20341 cggtcacgag gatcggctgc aggacgccgt gctcccgaat gcttgccgcg aggctgtcga
    20401 gctcgccctg gtccatccgc tgccgcggct ggcgcgggtt cggtcggatc cgcgcgatcg
    20461 ggatctcgct cgtcggcgca tgctggcttc gctgcgggat cagcgacgac aaaccgcggc
    20521 ccagccctga ctggcgctcg gagcgaacgg tcatcccggg actccgacgg cgagcgcggc
    20581 agtcgtcgcg gtcgaggtga gcagaggcga gccggacggg gtggcgaggt cggttgacgt
    20641 gatcggtgag ccgtggccgt cgcggcgtcg caactccgcc gccagcgctg cgtaggcgat
    20701 cgccccgcgc gagttgtcac ggtacagcgc gatcgggagc ccgaagctcg gcgcctctga
    20761 caggcgaacg ctacgcggaa tgatcgtgtc gaatactgcc gcgccgaggt ggttgcgaac
    20821 ctcggccgcg acgtccgccg agaggttggt tcgggcgtcg aacatcgtga gcacgacccc
    20881 cttgatcgcg agggccgggt tgaggtggtc ccgcacgaga ttgagcgtgg cgatgagctg
    20941 tgtcaggcct tccagggcgt agtactcgca ctgggtcggg atcaggaccg aatcggctgc
    21001 cgtcagcgca ttgacggtca gtaggccgag ggacggcggg caatcgatga ggacgtagtc
    21061 gtacgcgtcg aggactggtt ccaggagttt cgtcaggcgt cgttcgcgtt gatcggccgt
    21121 cgccagctcg acttccgctc cggcaagcga caccgaggcc ggcacgatcg agcacgcgct
    21181 gacgacggtc ggcaggacgg tctcggccat ggccgtgttg ccaaggagga cgtcgtacag
    21241 ggtgacctcg agagccgaac ggtcgatgcc gaagccgctc gtggcattgc cctgcgggtc
    21301 caggtcgacg atcaggacgc gctctccggc gagcgcgaga taggctgcca gattgaccac
    21361 ggtcgtggtc ttgccgacgc cgcccttctg attggcacag gcgatcgtct gagccactca
    21421 gaaccgccga atcgatgcgc tcacgaagat gaattctagc atcggaaggc cctcagcccc
    21481 ggtccggatt gagttccgat gatcgcgcgg acgcattcac ggccgctcac cggacggttc
    21541 acggtccatg accccgtccc atgcgcggtg atgcggtggc ggtggggttt cacgagaaac
    21601 ctcgcatccg ggaggccggc gacgacgggt cgacaacatg gcaagcaatg gtttcacgtg
    21661 aaaccgggcc aacagcccag ttgtccgccg gcgctcgatt gattcgggcc attgccgcgt
    21721 tcgacgctcg cgtgggatac tccgcgtgaa cgtcaccgcc gccgcaggtc gtcggaagtc
    21781 acgcacgatg cagggcgcga ccctgggctc gccgaggatc gaaccgacca tgaccttgct
    21841 cgtcgccgcg attgcagcga cggtggccgc actactcgaa tcgaccatca cgcaatacct
    21901 tcgggtgggc gacacggccc agccgcatct cgtcttcatc ctggcggtca tctggaccgt
    21961 cgccgctgga ctcgacagcg ggctcgtctg ggccttcgtc gggggcatcg ccctcgactc
    22021 cctcgctcag cgtccgctcg ggagcacagc gtttgccttg ctcattaccg tcggcgccac
    22081 gagcgttctc gcccgagcgt tgacccgggt ccgaccgatc gtgaccatca tcgcgactgc
    22141 cctcctgagc cttgtctatt ccatgaccct tgtcctcctc ttctcggtcc ttcgcccgcc
    22201 ggcggcgctc tcggatccgc tccggatcgt tctcccgagc gtcgcatacg acgttgtcgt
    22261 ggccgcgatc ctcgggccgc tgatcgtgtc gatccgtgac cggttcacgg acgaagagcg
    22321 agcgccccga tgaacccgta cctcgacggc cagccgatcg cgaagcggcc cggctcccgg
    22381 ttcctggtgt tcgggatcgc cgcgatcctg gcgatcagtg gcctgacgac ccgcctcttc
    22441 tatctccaga tcgtgagcgg cggccagttc gcggccctga cccaggtcaa ccgcgaggtc
    22501 gaacaggcga ttccatcctc gcgcggcctg atcttcgacc gcgccggtcg cgtgctcgtg
    22561 aagaacgtgc tgacctacgc ggtcaagctc cgacccgtcg acctgcccga ggagcgacgc
    22621 gacgaggtcg tcgcgaggct gtcggcgttg ttgaagatga acatcgcgga catcaacggg
    22681 gagatcgacg gcaacccggg atcgcggttc gatctcgtgc cgatcgcatc cgatgttccg
    22741 gagtccaccg ccaacctcat ctccgagtcg ggcgacgcct tgccgggtgt cgaagtcgtc
    22801 accgaggctc gccgccagta tcccgagggc aagctcgttt cgcagctcct cgggtacacg
    22861 ggtcccgtct cggccgacca actcgacacc ttgaagaagc agggctacct ggccgacgat
    22921 ctcctgggca agaccgggct cgaggcctac tacgagagca acctgcgcgg gctctacggc
    22981 agcgagaccg tcgagcgcga cgcggcaggg cggaagcttc aggtcctcga acggacccgg
    23041 gaaccccagg acggcgactc gctcaagctg acgatcgacg tgaaagaaca gcagtacgcg
    23101 cagaaggcgc tccagtgggg gatgcgtgca gcgggcctga agcgcggcgt cgtcatcgtc
    23161 atgaatcccc agacgggcga agtgctggca atggtcagcc tgccgaccta tgacgacaac
    23221 gcgttcgcca agggaatcag ctccaaggac ttcagcaggc tcttgaacga caagaacaag
    23281 ccgctcctga accacgccat ccaggcccac tacccaccgg gctcgacgta caagatcgtc
    23341 gcggggagcg gggccctcgc ggacaacaag atctcggcga cgagcctggt ccacacccgt
    23401 ggcttcctga tcctgggcag gaccaagttc tgggaatgga atcaccgcgg gtggggcgcg
    23461 atcccgatca agctcggctt cgcccattcc agcgacacct tcttcttcca gcttgcgggg
    23521 atgctcggca tcgagcgcct cgcccattgg gctcaccaat atgggttcgg cgcccccacg
    23581 ggcatcgatc ttcccggtga ggtggccggg atcgtaccga ccaacgcctg gaagcaggag
    23641 cacttcggcg agcccatcta tccaggcgag acctaccagg ccgggatcgg tcaggggtac
    23701 gacgtcgtca cgccgatcca gctgatcaac gcctacgccg cccttgccaa cggcggcaag
    23761 ctctattctc cgcagctcgt gcgcgagatc gtcgggccgg acgggtcggt cgtgcgccgg
    23821 ttcacgccca agctgatccg caagctcgcc gttccgcgga gcgacctcaa gacgatgcgc
    23881 gaggcggctc gcaacgtcct gctcgtccgc cacacctaca atctcgtcga cctgccgatc
    23941 gtgatcggcg gcaagtccgg aacggccgag ttcggtctcc gcgacagcaa gggtcgcctg
    24001 ccgttccact cgtggttcgt ggccttcgtt cccaagcatg ccgccaagac cgcgagcgac
    24061 ccgaccggga tgaaggcgat cgcgcgtgag gattcgaatc tcatcgtcct cgccttcgcg
    24121 tacgactcgc ggacgaaggg caacgcggcg accgagatcg tgaagtacta cctccagctc
    24181 cacttccaca tcaagcacga ctaccgcaac ttctacctgc tgcagcgcgg caacttctac
    24241 cagtcgaact gatggcgatg tcgacgagcc agggacgtga ccgccaggag gatctggcat
    24301 gggcgtgatc cgcaccgagc cggcccggat gaccgattgg gctgcccgct cgatcggtgc
    24361 ggcatggcgg gcgttcgatc tgcagctggc ggcgtacgcc gcgctcctcg cgatcctcgg
    24421 cctggtgatg gcctatacca acagcgtgga gcagggccag gcggtcctga gcgggggcac
    24481 gacgttcgtc cgcggcctga tgtggaccgg tatcgcgctc atcgcgttcg tcgtcacgac
    24541 cgccttcgat tacaagtggc tcaagacctt tgcctggccg ctctacctcc tccagatcgt
    24601 gctgctgatc gcaaccctcg cggtcggcca cggcgtcggg ggttcgtcgc gctggatttc
    24661 gttcggcccg ttcgacttcc agttcagtga gctggcgaag atcctgatga tcgtggtcct
    24721 ggcgaactac ctgggagccc gccagggccg actccggtcg ctgggctcca ttctgggggc
    24781 gtgcatcctc gtcgggccgc ccttcgccct ggtcatgctg cagcccgacc tcggcagctc
    24841 cctcgtgctc ctcgcgatcc tcgccggaat gctgttcatg tccggcgccc ggatgctctg
    24901 gctgacgatc ctcaccgtcg tcgggatggt gccgctgccc ctgatctggc aatacgtcct
    24961 gcacgactac cagaagcagc ggctgacggc gttcttcaac cccgcgagcg acacccaggg
    25021 cgccggcttc cagctcttcc agtcgcagat cgcggtcggt tcaggcgggc tgctgggcaa
    25081 ggggctcacg aacgggtccc agaaccaggc cagcctcctg ccggtccagg agagcgactt
    25141 cgtcgccgcc atctatctgg aggagctcgg cttcatcggc gcgatgatcc tgctgatcct
    25201 cttcggggcg ctgatctggc gcctgctcgt ctcaggctgg cggtcgaagg acccgttcgg
    25261 gatgatgttc gccagcggtc tcgcctcgat gatcctgttc cagctcgtgg tgaatctcgg
    25321 gatggtcatc gggatcatgc cgatcaccgg catcccgctg ccgttcatct cccacggcgg
    25381 ggcatcgctg atcagcctcg cggtcggtct cggcatcgtc cagagcatca acatccgaca
    25441 aacgcgcgcc gaatggtgaa tcgcagcggg accttgccca gcgcacgacc ggagcgcgtt
    25501 cgctgatcga ggtcggcgag ctcgggccga tgtggctgct ggtcgccttg ctcgcggccg
    25561 cggtcgcggt cgccatcgtg acccgccgct cggccctgcc gtacaccgtc ggactcgtcc
    25621 tcttcgggtt gaccctgtcc ctcatcggcc cggccgtcgc gttcccggtc agcccgaagc
    25681 tgctcctggc catcgtgttg ccggggctcg tcttcgaggc cgcgttccga acagacctcg
    25741 cgatcctccg gcgatcggcc ggctggatct tcctgctcgc gatcccgggc gtggtcgtgg
    25801 tcgcgaccat cgttgccgcc atcttgtcgg cgacgaccac cctgacgttc acggaatcct
    25861 tcctggtcgg cgcgatggtg gccgcgaccg atcccgcggc gatcctcggc acgttccgcc
    25921 agatccgggt gcccatccgg ctcgagacga tcgtggagat ggagagcctg gtcaacgacg
    25981 ggaccggcat cgtcctgttc gccgtggcgc tcgagatgct cgaggggggt ggctcgatcg
    26041 gcggctcggt cgtggtgttc gccgtcagcc tggtcgggag cgcgatcctc ggggccctgc
    26101 tcggggccgt ggccgctcgt atcgttcgcg gcatcgacga gcacctgacg cagctcacga
    26161 tcacggtcgt cctcgcctac ggcacctacc tcgccgccga tgcggtcggc ctgtcgggcg
    26221 tgatcacgac gctcgtcgcc gcaggcgcat tcgggaccat tgcgcggggc gacctgacca
    26281 gccagacccg ggacaccatc gacgtggtct gggagttcgc ggcgttcctc ctgacggccg
    26341 gtgtgtttct cctcgtcggg ctggcaatcg cgccgtcgac cctggcgctc gcggcggaac
    26401 cgatcgtgtg gggcgtcctc gcgatcctca tcgctcgggc gatcgtcgtg tacggactgc
    26461 tcgggatccc agcgacgctg ttcgcccgac ggggccgaca ggacctggcc atcccccgac
    26521 cgtggctgca cgttctgttc tgggccggcc tgcggggtgc ggtgtcggtc gcccttgccc
    26581 tctcgatccc gagcgacgtt ccgaaccgcg agctgatcca gggcgtcacc ttcgggatcg
    26641 tcctgttcac gctgatcgtc cagggatcga cggccggcct cgtcgtgcgc cgcaccggcg
    26701 cgtcgacgac gagctgacgg cggcggcgtc agcaccgatc gcgcggcgcg acacggctcg
    26761 cgatcagcgc cggcggaaca gccctcggcg ttcctgagcc tcggccgagg cactcttccg
    26821 ccggcccgac cgccggagga tgacgtcgag ctcgtcacga tagggccgcc cgatcggcat
    26881 ccggtcgagc cactcgatgt aatcgaggtc gcgccgggcg atctcgccga tcgaccagcc
    26941 ggcgaaccgc ccgaagttca ggacgcttcc ggacgggtcg cccggcggcg ggcctgcggc
    27001 gccgaggccg tcgggcgctc ccatccgggc cgggtcgtag ccaccgccct ggctcgagcg
    27061 accgctcgtc cagtcgctcg agaccgtttc agcgcggcgt ggtggatcgg cgcggcgtgg
    27121 cggatcggcg gcggggccgg gggaggtgcc tcggtcgctg ccggacgtgc ccggcgcggg
    27181 cgacctcgac gaagcgtgcg ctggctggtg tgccgcggaa cggcccgcgg ccgcggctcg
    27241 ctcggcgttg cgcgcggcga ggcgacgggc gagatcgtgg gcggcgcgct tcgccggatc
    27301 gcgaaggatc tcccacgcag cgttgatcgc cgccatccgg gcgtccgcat cgggcccggt
    27361 tgcgacgtcg gggtggtatt tctgggcgag cctgcggtag gccgcctgga tgacctcgtc
    27421 ctcggcctcg gaatcgacct ggagaacctt gtagggatcg gggccgtcgg tcatcgcctc
    27481 aggcctcgag ctcggcgtct tcggcgtcgg cggcgtcggc ggcgggttcg ggcaccggtt
    27541 ggacgatggt cggacgcgcc cggatgcgcg ccaccgcctc gtccggggtg gccggcgggt
    27601 cgtcgaagcg atggaagaga tagcggaaga catcgcgggc cgcggcactg atcggcaggg
    27661 cgaggatcgc gccgagcaat cccgcgatcg ccccgccgat cacgagcgcg aacatcaccg
    27721 cgctcgggtg gagctcgacc gcgtcgccct ggatcttcgg cacgaggaag tagttctcga
    27781 gctgctggat tcccgtatag aggatcaccg ccgcgaccgc cacgtccagg ccggccgtca
    27841 gggccagcag caccgccggg atcgccgcga tgatcggacc gatgatcggc agcagctcga
    27901 gaacaccggc aacgaccgac aggaggatgg cgaagcggcc gaagatcggg tcgaccgtca
    27961 cgctcaggag gagcaggccg gcgaacgtcg ccgccccgac cgtgacgccg aggacgagct
    28021 ggccgcggac ccactggccg aagacgcgca ggaccaggct gctcactgcg ctgacgtcgg
    28081 gccaccaggc atcgggcatc gaccgctcgg cggcggccgt gagagccgga cggtccttga
    28141 tcaggtagaa gatccagacc gggatgagga catagccgac gatcgatccg acgatcccgg
    28201 cgatcccgct gacgaccggc aggaccgcgg ccggatcgat cccggcgatc cctgagccga
    28261 gatcggcgag ccaggtgtcg atggcgtggc ggacggccgg cagcaggtcg aggtgggcgt
    28321 agatgtcggt cacctgcgcg gtgaacttcg ggaactcgcg gatgaacgtc gcgaattcat
    28381 cggacaacgg ccgcatcgcc atgacgccgc cctcgacgac caagacgacc acgacggcgt
    28441 agacgatcag gaccgccagc cagcggggga gcccgatccg cgacatccgt tcgaccggca
    28501 tgtcgaggag ataggcaagc acgaggccga cgacgaacgg cccgagggca gagctcccgg
    28561 cgtggaggac gacggccacc accgccgagc cggcaaggac gaggacgacc cgcggactga
    28621 gcggcccgaa gccggctcgc gcctggtcgg tcggcgccaa tacccgctcc tccatcgctc
    28681 gggcagacta gcagacgtga ttgggcccac cgaccccacg ccgccgcggc ggtgctg
//
LOCUS       DATAIW010000216         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_100834, whole genome shotgun sequence.
ACCESSION   DATAIW010000216 DATAIW010000000
VERSION     DATAIW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100834"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..920)
                     /locus_tag="VHM48_06945"
     CDS             complement(144..920)
                     /locus_tag="VHM48_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755182.1"
                     /translation="MLRRRTIAGERIFRTAEVLAYRCDLASVEVNAVVEARFGTGDAV
                     DRGIDRTIALFDGRPFLWWVGEDDTPSDLSDRLDRRGVIFLDEIPGMAMDLADLAEPV
                     DSRPSGLEIEPVLDAAAMEAFHSVLVQGFSEDFVDPSVNAAIGASSVRAAIEMSYREP
                     NGLPTRWIGRVDGRPVTTTRLHTAAGVAGIYAVVTAEGARRRGYGEAITRRVLRVARD
                     GGLRIAVLQASPAGRGVYERIGFREQCRLRLHEWPATAFP"
     gene            complement(1064..1438)
                     /locus_tag="VHM48_06950"
     CDS             complement(1064..1438)
                     /locus_tag="VHM48_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755183.1"
                     /translation="MTVRVGALLWPQTNSWPVLRDAEIRDDRAGLDSLWTWDHLMAIV
                     GPSEQPILEGWTTLAALASITERPTLGLMVGANTFRNPGLTAKLRPTVEAGLDHLIVD
                     AMAPYDAETLERLPEVRKLLDG"
     gene            complement(1500..>1704)
                     /locus_tag="VHM48_06955"
     CDS             complement(1500..>1704)
                     /locus_tag="VHM48_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755184.1"
                     /translation="FEQMAKLVRPEDFDGRLLISSDPDQHRAQIQRFLDLGFDQIYLH
                     NVGRNQAEWIDVFGREVLPKLTA"
BASE COUNT          251 a          597 c          620 g          236 t
ORIGIN      
        1 agccggctgc ctcggccgcg atggcggcat cgcggaagtc cgaccaggtg gtctgctggg
       61 accagagctg agcaccgaac cgaatcgtca tgtcgacatc atccggcatc gagcccgggg
      121 gcgtgccggc tggccggcgc cggtcaggga aacgccgtcg ccggccactc gtggaggcgg
      181 agccggcact gctcgcgaaa cccgatccgc tcgtagactc cgcgcccggc cggagacgcc
      241 tggaggaccg cgatccggag gccgccgtca cgggcgaccc gcaggacccg gcgggtgatc
      301 gcctcgccgt agccgcgccg ccgggcgccc tcggccgtga cgacggcata gatcccggcc
      361 acgcccgccg ccgtgtggag ccgggtcgtc gtcaccggtc ggccgtcgac cctcccgatc
      421 cagcgggtcg gcagtccgtt cggctcccga tagctcatct cgatcgccgc tcggacgctg
      481 gacgcgccga tcgcggcatt gaccgagggg tcgacgaagt cctccgaaaa tccctggacg
      541 agcacggagt ggaaggcctc catcgccgcg gcgtcgagga ccggctcgat ctccagcccg
      601 gacggtcggc tgtcgaccgg ctcggcaagg tcggcgaggt ccatcgccat gcccgggatc
      661 tcgtcgagga agatgacgcc acgacggtcg aggcggtcgc tcaggtcgga cggcgtgtcg
      721 tcctcgccga cccaccacag gaacggccgg ccgtcgaaca gcgcgatcgt ccggtcgatc
      781 ccccgatcca ccgcatcgcc cgtgccgaac cgcgcctcga cgaccgcatt gacctccacg
      841 gacgcgaggt cgcatcggta ggcgagcacc tcggcggtcc ggaagatccg ctcgccggcg
      901 atggtccggc ggcggagcac cgaacggacg agatcctcgt ggatcgcgtc ccgaagcgtg
      961 gcgggagacg ggtccgttcg cggttcggag gcgacggccg cgggcacggt ttcgcccgcg
     1021 acggccgtcc ccgccgccgg ttccgttgca cgcgccgccg gcgtcatccg tcgaggagct
     1081 tgcggacctc gggcaggcgc tcgagggtct cggcgtcgta gggcgccatc gcgtcgacga
     1141 tgaggtgatc gaggcccgct tcgaccgtcg ggcggagctt cgccgtcagg cccggattgc
     1201 ggaaggtgtt ggcgccgacc atcagcccga gcgtcggccg ctcggtgatc gaggccaggg
     1261 cggccagcgt cgtccagccc tcgaggatcg gctgctccga cggcccgacg atcgccatga
     1321 ggtggtccca ggtccagagg ctgtcgagcc cggcccggtc gtcccggatc tcagcgtcgc
     1381 gaaggaccgg ccagctgttc gtctggggcc agagcagggc gccgacgcga acggtcatcg
     1441 agatcctccg atcggttggg caggcacgcc ggctcgagcg cgatgaaggc ggccgagcgt
     1501 cacgccgtga gcttgggcag gacctcgcgg ccgaacacgt cgatccactc cgcctggtta
     1561 cgaccgacgt tgtggaggta gatctggtcg aagccgagat cgaggaatcg ctggatctgg
     1621 gcccggtgct ggtccgggtc ggacgagatc agcagccggc cgtcgaagtc ctcgggccgg
     1681 acgagcttcg ccatctgctc gaag
//
LOCUS       DATAIW010000217         6760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_160821, whole genome shotgun sequence.
ACCESSION   DATAIW010000217 DATAIW010000000
VERSION     DATAIW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160821"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..4083)
                     /locus_tag="VHM48_06960"
     CDS             complement(127..4083)
                     /locus_tag="VHM48_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2755185.1"
                     /translation="MASGFRRSLARRGGRTVAALVAIVVVGQAGLVPTATALTHLVAE
                     AQAVRIGAGDREPPSIDAAERGPTVDLAAFDGAPVALDPVAAPDPGDPADPGDPGGSD
                     AADSYAGLQPSIQYEEAERHATDRIAFTPGNRVTVGFQPRPGDHWSVGGLSPTTLPAG
                     RLDGRTIRAQGASAVIKRPSRPDRGPATTVPATPVPADRAADSAVDLPSDTATPVAAT
                     SAAFQAPLGAGAATPTIQPDAVVKPDGLRREIFGFLPYWEVNSSSLRLDYSKISTVAY
                     FGVGADGAGNLQKRNSDGSITVGWSGWTSSSMTSVISAAHASHTRVVLTVQSFGWSTS
                     GLTRQKSLLGSSTHRLNLARQIAAAVRDRGADGVNLDFEPLASTYDNEFTALVRTIRA
                     ELNKVHSGYQITFDTLGSIGNYPIADATAPGGADAIFIMGYDYRSASSNPVGSIAPLT
                     RTGYDIRDTILAYTARVAPSKLILGVPYYGRAWSTATGALRATNTSGTKYGASTAVVY
                     DTAADVLAQHGRHYDATEGVAWTAYQRENCTSTYGCVTSWRELYVDDATAIGAKYDLV
                     NTYRLRGAGIWALGYDGTRPELWAAIQRKFVTDTTPPIAGVRTLPARQVNPGFTVTWT
                     GRDDVAVASYDVQVSVDGGAWVTWLAATKATSAAWSGFDTHAYAFRVRARDPKGNLGT
                     WNVTSTSPSAGAGLAAGGFGIVRVDALSIRSAPGTAATVIGQYASGDVVAIVAGPRSV
                     DGYTWFQVVGPLTEWGAVAARDPAAWIPTSGGRMSPAKAPNTTRVGAAIGGLGFGNAG
                     PASIGSAAAAVAHRAFSPNGNHSGDTLALDWTNDRAFDSLSLRVFKADGTLLGSVPVN
                     QLAAGARQAAWDGKVGGSTLPNGRYLVSLVGTAGGRAFYNPVTSFRSAALAAYGVTID
                     TVAPTITSSSVSSALISPNGDGILDTIRVTLGATGADGWTFSASPLAGSTAGAPVTTR
                     SGTGGSAAVAWNGRTNGGAVVPDGTYRLTLSATDHAGNRTPRAWTVRVDHTPAAIQAT
                     ATPATFSPNGDGAADTTRLGWTASERISGLARVVHGTTVIRSWTVSGAMAGSIAWNGR
                     TAAGTVVADGTYTFRVTGRDAAGNLAVRTVPITVDRTLSSVRWSRSAFFPQDGDALTP
                     TAAFSFSLKRGAVVTVGIYSGSTLVRMVCTGRSFAAGAHSWTWDGKDSHGAFVARGTY
                     SVRVTARSWVGTSVVSRSILVDAFRIALSATSVSAGQTLIVTVTTTEGLRSAPVIAFS
                     RPGRAVVTRTATALGSGQYRATFVVAAGTAGKATIRVTGRDSGGGLNTSTRSVTIR"
     gene            complement(4284..6590)
                     /locus_tag="VHM48_06965"
     CDS             complement(4284..6590)
                     /locus_tag="VHM48_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX2755186.1"
                     /translation="MRNLAAAIVIALITALGIAGPVAAVQTSTAKVVIIVGATHGATA
                     GYRSNADAAYNEAIKYTSHVTKVYSPNATWSKVKAAVYNANIVIYFGHGNGWPSPYTY
                     DPTYTTKDGMGLNADLNGDGKLSDYENKYYGEPYMAQLHLAPNAIVLLHNLCYASGNS
                     EPGGAAPSVGVARQRIDNYAAGFLKGGARAVIADGHMGPPNYLAGLFTTSRSIVDLWR
                     SMPNYHGHESSFASTRSPGYMDYSDPDNATSSFYRSLVVKPAMTTTAVTNAVGDTGLD
                     PASLVVPGRASVEAPAAPLFATATAIATTDAPSLLSLPAGTRLKTLATAIPAAGGSPA
                     VIQVVGLDDAGIAGYVSAADLAPRDSRAPVLIGIDSGAGRFSPNDDGRSDEQTMTGIF
                     SEIVDWTVDVKDADGTVLDTTTGHGRTFSVPWDGLVDGIAVRDGSYAWTARGIDAWQN
                     GTARGSGTVVVDTVGVDVSAISPDGSSVGVFSPNGDGVADTVSTTVSVPEAGSIVGWV
                     TDGDGTTVRSWTATSIAGANPMIWDGRAANRAYVPDGDYTLHVTARDRTGTNGAGRTR
                     DVRVIGFLSAVRSSVRAFYPQDADRLATTTALSFALAAPATVTWTIRDAANNIVVTHL
                     QDAAVAAGTQTWTWDGRTAAGALLPLGAYSSSVTASDGTRTISQTAKFDMNAFAIATS
                     TSTPHRGSKMTVVVTSAEPLSSGVIMYVAQTGISTWHVTLTKIDSRTYKATITLKTGG
                     SAGTVRLTVRGRDLDGRTQSSIRTLALS"
BASE COUNT          922 a         2276 c         2537 g         1025 t
ORIGIN      
        1 aggttctcgg cctcgttgta cgtcggcagg acgatccatg cgccgcggcc ggccgcggcc
       61 tcattggacg gatgctgagt cgatggcccg gcggcggtcg tcatcgcccg ccagtgtatc
      121 gggcggtcat cgaatcgtga ctgacctcgt gctcgtgttg agcccaccgc cgctgtcccg
      181 gccggtcacc cggatcgtgg ccttcccagc cgtgcccgct gccaccacga acgtggcccg
      241 gtactggccc gacccgagcg cggttgccgt ccgcgtcacg accgcacgtc ccggccggct
      301 gaacgcgatg acgggtgcgg agcgcagccc ctcggtcgtg gtcacggtca cgatcagcgt
      361 ctgaccggcc gagaccgacg ttgcggagag cgcgatccgg aaggcgtcga cgaggatcga
      421 tcggctcacg accgacgtcc cgacccagct ccgcgccgtg acccggaccg agtacgtgcc
      481 acgggccacg aatgcgccat gcgaatcctt gccgtcccac gtccagctgt gcgcgccggc
      541 ggcgaaggag cggcccgtgc agaccatccg gacgagggtc gagcccgagt agatgccgac
      601 cgtcacgaca gcgccccgct tcaggctgaa cgagaacgca gccgtcgggg tcagggcgtc
      661 gccgtcctgc gggaagaacg cggagcggct ccagcgaacg ctcgagagcg tccggtcgac
      721 ggtgatcggg acggtccgga ccgcgaggtt tccggcggcg tcgcgacccg tgacgcggaa
      781 cgtgtaggtg ccgtcggcga ccacggtacc ggcggcggtc ctcccgttcc aggcgatcga
      841 cccggccatc gcgccgctga cggtccagga ccggatcacc gtcgtgccgt gaacgacgcg
      901 ggcgaggccg ctgatccgct cggacgcggt ccatccgagc ctcgtcgtgt ccgccgcgcc
      961 atcaccgttc ggagagaatg tcgccggcgt cgccgtggcc tggatcgcgg ccggtgtgtg
     1021 gtcgacccgg acggtccagg ctcgcggcgt tcggtttccg gcatggtcgg tcgcgctgag
     1081 ggtcaggcgg taggtcccgt ccggcacgac ggcgccgccg ttcgtccggc cgttccaggc
     1141 gaccgcggca ctgccgcctg ttccggagcg cgtggtgacc ggcgccccgg ccgtcgaacc
     1201 ggcgagcggc gacgcgctga acgtccagcc gtcggcgccg gtcgcgccca gggtgacccg
     1261 gatcgtgtcg aggatcccgt cgccgttcgg cgagatcaac gcgctgctga ccgacgagga
     1321 ggtgatggtc ggcgcgaccg tgtcgatcgt caccccgtac gcggccagcg cggcagagcg
     1381 gaacgatgtc accgggttgt agaacgcgcg gccgccggca gtgccgacca gcgagacgag
     1441 atagcgtccg ttcggcaggg tcgaaccacc gaccttgccg tcccaggcgg cctgccgggc
     1501 gccggctgca agctggttga ccggcacgct gccgagcagc gtcccatccg ccttgaagac
     1561 gcgcagggac aggctgtcga acgcccgatc gttcgtccag tcgagggcga gcgtgtcgcc
     1621 cgaatggttg ccgttcggcg agaaggctcg atgggcgacc gcggccgccg ccgacccgat
     1681 gctcgccggg ccggcgttgc cgaacccgag gccgccgatc gcggccccga cccgggtcgt
     1741 gttgggcgcc ttggccggcg acatccgacc gccgctcgtc gggatccagg cggccgggtc
     1801 cctggccgcc accgcgcccc actccgtgag ggggccgacg acctggaacc aggtgtagcc
     1861 gtcgacggag cgcggcccgg cgacgatcgc gacgacgtcg ccgctcgcgt attgaccgat
     1921 gacggtcgcc gccgtcccgg gcgcggaccg gatcgacagg gcgtcgaccc ggacgatgcc
     1981 gaaccctccg gcggcgaggc ccgcgccggc gctcggcgat gtcgaggtca cgttccacgt
     2041 cccgaggttg cccttcgggt cgcgagcccg gacgcggaac gcgtaggcat gggtgtcgaa
     2101 gccggaccag gcggccgacg tggccttcgt tgccgccagc caggtcaccc aggcgccgcc
     2161 gtcgactgag acctggacgt cgtagctggc aacggcgacg tcgtcgcggc cggtccaggt
     2221 caccgtgaag cccggattca cctggcgagc cgggagcgtc cggacgccgg cgatcggtgg
     2281 cgtcgtgtcg gtgacgaact tgcgctggat cgccgcccag agctccggcc gcgtcccgtc
     2341 atagccgagc gcccagatcc ccgccccgcg cagccggtag gtattgacga ggtcgtactt
     2401 ggcgccgatc gcggtcgcgt cgtcgacgta gagctcgcgc cacgacgtca cgcaaccgta
     2461 cgtcgaggtg cagttctcgc gctggtatgc ggtccacgcg accccctcgg tcgcgtcgta
     2521 gtggcggccg tgctgggcga ggacgtcggc cgcggtgtcg tagacgaccg cggtcgaggc
     2581 gccgtacttc gtgccgctcg tgttcgtcgc gcggagggcg ccggtggccg tcgaccaggc
     2641 ccggccgtag tacgggacgc cgaggatcag cttcgagggc gccacccggg cggtgtaggc
     2701 gaggatcgtg tcgcggatgt cgtagccggt ccgggtgagc ggcgcgatcg agccgaccgg
     2761 attcgaggac gccgagcggt agtcgtagcc catgatgaag atcgcgtccg caccacctgg
     2821 cgccgtggca tcggcgatgg ggtagttgcc gatcgagccg agcgtgtcga aggtgatctg
     2881 gtagccgctg tggaccttgt tcaactcggc ccggatcgtc cggacgaggg cggtgaactc
     2941 attgtcgtag gtcgaggcga ggggctcgaa gtcgaggttc accccgtcgg cgccgcggtc
     3001 gcggacggcc gccgcgatct gcctcgcgag gttcaggcga tgggtcgacg agccgagaag
     3061 gctcttctgg cgcgtcagcc cgctcgtgct ccagccgaag ctctggaccg tcaggacgac
     3121 acgcgtgtgg gaggcgtggg cggccgagat gacgctcgtc atcgacgagc tggtccagcc
     3181 gctccaaccg accgtgatcg aaccgtcgct gttgcgcttc tggaggttgc ccgcgccgtc
     3241 cgctcccacg ccgaagtagg cgaccgtcga gatcttgctg tagtcgagcc ggaggctgct
     3301 cgagttgacc tcccaatagg gcaggaaccc gaagatctcg cggcgcaggc cgtcgggctt
     3361 gacgaccgcg tccggctgga tcgtcggcgt cgcggcgcca gctccgagcg gcgcctggaa
     3421 cgcggcgctc gtcgcggcca ccggcgtcgc ggtgtcgctc ggcagatcga ccgctgaatc
     3481 ggcggcccga tcggctggga cgggcgtcgc cgggacggtc gtcgccgggc cacggtccgg
     3541 tcgggacggt cgcttgatga cggccgatgc cccctgggcg cggatcgttc gaccgtccag
     3601 gcggccggcc gggagggtcg tcgggctcag gccgccgacg gaccagtggt cgccggggcg
     3661 gggctggaag ccgacggtca cgcggttgcc cggggtgaag gcaatccggt cggtcgcatg
     3721 ccgttcggct tcctcgtact ggatcgacgg ctggaggcct gcgtacgaat cggcggcgtc
     3781 ggatccgccg ggatcgccgg gatcggcggg atcgccggga tcgggcgcgg cgaccgggtc
     3841 gagcgcgacg ggcgcgccgt cgaacgcggc cagatcgacg gttgggccgc gctcggcggc
     3901 gtcgatcgac ggcggttcgc gatcgcccgc tccgatccgg acggcctgcg cctcggcgac
     3961 gaggtgggtc aacgcggtcg cggtcgggac gagcccagcc tggccgacga ccacgatggc
     4021 gacgagcgct gcgacggtcc gaccaccgcg tcgagcgagg gagcggcgga atccggatgc
     4081 cacgaatgtc ctctcgaacg gtttccccat cgccccggcg acggctcttc gacggtgcga
     4141 gcgtggaacc tgtcgccacg caccggactc gatcgaccat acgcccgcca ggcaacctcg
     4201 gaaaccggcc cgatggtcct gatcgtgtgc agtacggtgg ggtccgatgg ggtctgggac
     4261 cctccgtccc gcgaggcctg cgctcagctc agggcgaggg tcctgatcga cgactgggta
     4321 cgcccgtcga ggtcgcgacc ccggacggtc agacggacgg tcccggcgga gccgccggtc
     4381 ttgagcgtga tcgtcgcctt gtacgtccgg ctgtcgatct tcgtcagggt cacgtgccag
     4441 gtcgagatgc ccgtttgcgc gacatacatg atcacgccgc tcgagagggg ctcggcggag
     4501 gtcacgacga cggtcatctt gctgccgcga tggggagtcg aggtcgaggt ggcgatcgcg
     4561 aacgcgttca tgtcgaactt ggcggtctgg ctgatcgtcc gggtgccgtc agacgccgtg
     4621 accgacgagg agtacgcccc gagcggcagg agcgcgccgg cggcggtgcg tccgtcccag
     4681 gtccatgtct gcgtgccggc cgccaccgca gcgtcctgga ggtgggtcac gacgatgttg
     4741 ttcgccgcgt cacggatcgt ccaggtcacg gtcgcgggcg cggcgagggc gaaggacagc
     4801 gccgtcgtcg tcgcgagccg gtcggcgtcc tgcgggtaga aggcccgcac ggacgagcgg
     4861 accgcgctca ggaagccgat gacgcggaca tccctggtcc tgcccgcccc gttggtgccg
     4921 gtccggtcgc gggcggtgac atggagcgtg tagtcgccgt cggggacgta cgcgcggttg
     4981 gccgcccggc cgtcccagat catcgggttg gcgccggcga tcgacgtcgc ggtccacgat
     5041 cgtacggtcg tgccgtcgcc gtccgtgacc cagccgacga tcgagcccgc ctcgggcacg
     5101 gacaccgtcg tcgagaccgt gtccgcgacg ccgtcgccgt tcgggctgaa gacgccgacg
     5161 ctgctgccat cggggctgat cgcgctgacg tcgacgccga ccgtgtcgac gacgaccgtg
     5221 ccgctacctc tggccgtgcc gttctgccag gcatcgatgc cgcgtgcggt ccaggcgtac
     5281 gacccgtccc ggacggcgat gccgtcgacg agcccgtccc acggcacgct gaaggtgcgg
     5341 ccgtggcccg tcgtcgtgtc gagcacggtg ccgtcggcgt ccttgacatc gaccgtccag
     5401 tcgacgatct cggagaagat cccggtcatc gtctgctcgt ccgatcggcc gtcgtcgttc
     5461 ggcgagaacc gcccggcacc cgaatcgatc ccgatcagga ccggcgcccg gctgtcgcgc
     5521 ggcgccaggt cggcggcgga gacatagccc gcgatgccgg cgtcgtcgag gcccacgacc
     5581 tggatgaccg ccgggctccc gcccgccgcc ggaatcgcgg tcgcgagggt cttgagccgg
     5641 gtgcctgccg gcaggctgag cagactcggc gcgtcggtcg tggcgatggc ggtcgcggtg
     5701 gcgaagagcg gggccgcggg cgcctcgacg ctcgcccggc ccggaacgac caggctggcc
     5761 gggtcgaggc cggtatcgcc aaccgcgttg gtgaccgcgg tcgtcgtcat ggcgggcttc
     5821 acgacgagcg agcgatagaa gctgctggtg gcattgtcgg gatcggagta gtccatgtaa
     5881 cccggcgagc gggtcgacgc gaatgacgat tcgtggccgt ggtagttcgg catcgagcgc
     5941 cacaggtcga cgatcgaccg gctggtggtg aacaggccgg cgaggtagtt tggggggccc
     6001 atgtggccgt cggcgatgac ggcccgggcg ccgcccttga ggaagcccgc ggcgtagttg
     6061 tcgatccgct ggcgggccac tccgaccgac ggggccgcgc cgccgggctc ggagttgccc
     6121 gaggcgtagc acaggttgtg gagcaggacg atcgcgttcg gcgccaggtg gagctgggcc
     6181 atgtacggct cgccgtagta cttgttctcg tagtcggaca gcttgccgtc accgttcagg
     6241 tcggcattga gccccatccc gtccttggtc gtgtacgtcg ggtcgtaggt gtaggggctc
     6301 ggccagccgt tgccgtggcc gaagtagatg acgatgttgg cgttgtagac ggccgccttg
     6361 accttcgacc acgtcgcgtt cgggctgtag accttcgtga cgtgggaggt gtacttgatc
     6421 gcttcgttgt aggcggcgtc ggcgttcgat cggtagcccg ccgtcgcgcc gtgggtcgcc
     6481 ccgacgatga tcaccacctt ggcggtgctc gtctggaccg cggccaccgg accggcgatc
     6541 ccgagggcgg tgatgagggc gatgacgatg gcggcggcga ggttgcgcaa tgcagctccg
     6601 aagcgaaacg aacggccgcg ggcgcgagct gccggcggac gtggatgaat gccctcgaag
     6661 cgtcctcctc gcccgcgccg aaaccaatcg cgggatggtc ccgccggtca tgggcccttc
     6721 ggacggcgcc ggatacggag atgggtggag gggtgcggtc
//
LOCUS       DATAIW010000218         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_124193, whole genome shotgun sequence.
ACCESSION   DATAIW010000218 DATAIW010000000
VERSION     DATAIW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124193"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..2135)
                     /locus_tag="VHM48_06970"
     CDS             complement(411..2135)
                     /locus_tag="VHM48_06970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755187.1"
                     /translation="MADLPLLVFGPQSLDYDFGSTHPLTPRRFGPGMELLRAVGAEPG
                     LAPEPATDDELLMAHTRHYLDVVKRFSEFDHGWMEPEAGIGVGGDDPPFHGMHDAAAA
                     VAGGSLRAIEAILRGDVEHAFHPGGGLHHAMPSRASGFCIYDDPALAIAWARREGMRV
                     LYVDLDVHHGDGVETIHAADPGVLTLSFHESGRYLFPGTGFTDEIGVGAAAGTVVNVP
                     LEPDTGESAWLDAVRSLLPLLAAAFGPDLIVSQHGADSHAWDPLAHLRNTTTAMGEAA
                     RLVDAIAHRVAGGRWLATGGGGYGVYSVVPRSWSLVWLAAAHREVPDRLPAAWRERWA
                     GEAARYGTAALPTTFIDPPNAGDPASRSQDAAERLSRTTAATVREVLVPGLLRSAADR
                     GWWDPLALVESDGSGAVREPDGAATASILPGVDLAIWEHLVPAAQVIPPARTTDARAL
                     ISAALRSGARVTAAVVDGRVAVGLAVSGPDTDGSEDLLALGVAPAFRRQGLAARLLAA
                     HVEAARPGRSIRARVGVAERDAVEPLEHATRIAIARRLLEGAGFTVEPAPDRIGRLDP
                     LAIAAIRR"
     gene            2155..2583
                     /locus_tag="VHM48_06975"
     CDS             2155..2583
                     /locus_tag="VHM48_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755188.1"
                     /translation="MERVYTTPLLDKLGIRPGMRVAIVGVLEDDDEVLDDEGPFRERL
                     AGRTSDITDGRPEPETDIVILAADTTAELVALADLRARIRPTGAIWVVSRKGRAATLR
                     DVDVIAAAREHRLVDNKVVSFSTRRTALRLVIPVALRLKG"
     gene            2602..2772
                     /locus_tag="VHM48_06980"
     CDS             2602..2772
                     /locus_tag="VHM48_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2755189.1"
                     /translation="MGSDVLVALIVVLVVVLIWRGPKTLPKIGQALGRGVREARTEAA
                     KLRDGKREDPPA"
     gene            2828..3430
                     /locus_tag="VHM48_06985"
     CDS             2828..3430
                     /locus_tag="VHM48_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX2755190.1"
                     /translation="MNEAIPPSVESLLERPGPGPHRTTIVVDGAAVDTLADLPPLRDR
                     LLFVGLNPSPVSVEAGHYFQGRLGQLFWRRLMTASILPPDTAIASADDALVAAGHGLT
                     DLLKVPTPRDEASDATLRAGVGPLWQKVAIWRPAAIVFVYKRAAEIVAARPLAEPWGQ
                     LAGVAMSGRPCFLMPGPYAPQAEVAAALNFVRNLGASLPR"
     gene            complement(3416..>4276)
                     /locus_tag="VHM48_06990"
     CDS             complement(3416..>4276)
                     /locus_tag="VHM48_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2755191.1"
                     /translation="ALTPADHASGAVGAASRALGDNLPPNEALAVHRAALRELREEAG
                     VTLDGIERLAPIAHWSTPAFMARRFSTWFFVADLPPEAVPAFAPDEVAAHRWVTPSQA
                     LDQVAAGEIDMWVPTTCVLERLIETGASKSSEVADRITLGPVAPPRVDSETPTVVRFT
                     FGAVGALPGRTGATTLYGRRELVLVDPGDPSDAALNLIGAAVQRRGGTINAIVLTATD
                     PDHAAAAQSLAIPLEIPILVAPGAGRHLPYPTVELGDGDLLPADVELRVWLGPPGSGR
                     LEIAPDSAGE"
BASE COUNT          611 a         1570 c         1538 g          557 t
ORIGIN      
        1 acccggtcct cttcaccatg gcgatcggcc tgatgctcgc ctggaaggtc gccggctaca
       61 tcggcgtcga ccgctacctc ctcccgatgc tcggcacacc gtggcgagcc aaggtggaca
      121 ccggacgacc gattgcggcg aatcccgcac caagctgacc aacggctctc ccgcaccaag
      181 gacccggccc ccgaccgggt cctttggatt ccttgttccg cgttcaaggc cgcccagctc
      241 cggcgcaccc ggcgcggtcg gccgatgccg tgcgggcact gatggaacca gacttgccgt
      301 ggcccgaagc cgcgatcatc tgggccgatg tgaccggcga ccgcgctcgg atcctgccag
      361 gcccgccacg cggagtcggg ttgggagcct gatctgtcgc gcgtgttcgc tcagcgccgg
      421 attgccgcga tcgccagcgg atcgaggcgg ccgatccggt cgggggccgg ctcgaccgtg
      481 aagccggcgc cctcgagcag acggcgggcg atggcgatcc tggtcgcatg ctcgagcggc
      541 tcgacggcgt cgcgctccgc gactccgacc ctggcgcgaa tcgaccggcc cggtctggcc
      601 gcctcgacat gcgccgccag cagccgggcg gcaaggccct gccggcggaa cgcaggtgcg
      661 acgccgagcg cgagcaggtc ctcggaaccg tcggtgtcgg gaccagacac ggccagcccg
      721 accgcgaccc ggccgtcaac gaccgccgcc gtcacccggg ctcccgatcg gagcgccgcg
      781 gagatgagcg cgcgggcgtc cgtcgtccgc gccggtggga tcacctgcgc ggccggaacc
      841 aggtgttccc agatcgcgag atcgacgccg ggcaggatcg acgcggtcgc ggcgccgtcc
      901 ggctcgcgga cggcgcctga accgtcgctc tcgaccaggg caagcggatc ccaccagccg
      961 cggtccgcgg cggaccggag gagccccggc acgagcacct cccggaccgt ggccgcggtc
     1021 gttcgcgaca gccgttccgc cgcgtcctgc gatcgcgacg ccggatcgcc cgcattcggg
     1081 ggatcgatga acgtcgtcgg cagcgcggcg gtcccgtagc gggcggcctc gccagcccag
     1141 cgctcgcgcc aggcagcggg cagccggtcc gggacctcgc gatgagcggc cgcgagccag
     1201 accagcgacc acgatcgggg cacgaccgaa tacaccccgt agccaccgcc cccggtcgcc
     1261 agccagcgcc cgcctgccac acggtgcgcg atggcatcga ccagccgggc ggcctcgccc
     1321 atcgcggtgg tcgtgttgcg aagatgggcc aatggatccc aggcgtggct gtcggcgccg
     1381 tgctgggaca cgatgaggtc gggcccgaac gctgccgcca gcaagggaag cagcgagcgg
     1441 acggcgtcca gccaggccga ttcgcccgtg tcgggctcca gcgggacgtt gacgacggtt
     1501 cctgccgccg ctcccacacc gatctcgtcg gtgaacccgg tgccggggaa gaggtatcgg
     1561 ccgctctcgt ggaacgacag cgtcaggacc cccgggtccg cggcatggat cgtctcgacg
     1621 ccgtcgccgt gatggacgtc gagatcgacg tacaggactc gcatgccctc gcggcgggcc
     1681 cacgcgatcg ccagtgcagg gtcgtcgtag atgcagaagc ccgacgcccg cgacggcatc
     1741 gcgtggtgga ggccgccgcc cgggtggaag gcgtgctcga cgtcgccgcg gaggatcgcc
     1801 tcgattgccc gaagggagcc gccggcgacc gcggctgccg cgtcgtgcat cccgtggaac
     1861 ggcggatcgt cgccaccgac cccgatcccc gcctctggct ccatccagcc gtggtcgaat
     1921 tccgagaagc gcttgacgac gtcgaggtag tggcgggtgt gggccatcag caactcgtcg
     1981 tcggtcgccg gctccggagc gaggccgggc tcggcgccga cggcgcggag gagctccatc
     2041 cccgggccga accggcgtgg cgtgagggga tgggtcgatc cgaagtcgta gtcgagcgac
     2101 tgcgggccga agacgagcag cggcaggtcg gccatggatg gcacgatagc ggcgatggaa
     2161 cgggtctaca ccacgcccct cctcgacaag ctgggaatcc ggccggggat gcgggtcgcg
     2221 atcgtcggag tgctcgagga cgacgacgag gtcctcgacg atgaaggacc gttccgggaa
     2281 cggctcgcgg ggcgaacgag cgacatcacc gacggccgcc cggagccgga gaccgacatc
     2341 gtcatcctgg ccgccgacac gaccgccgag ctcgtggcgc tcgcggacct tcgcgcccgg
     2401 atccggccga ccggtgccat ctgggtcgtg tcgcgcaagg gcagggccgc gacgctccgc
     2461 gacgtcgacg tcatcgccgc cgcccgcgag catcgcctgg tcgacaacaa ggtcgtctcg
     2521 ttctcgaccc gccggacggc gctccgcctc gtgatcccgg tggcgctccg actgaagggt
     2581 tgaggcgagt accatccgcc catgggatcg gatgtcctcg tcgccctcat cgtcgtgctg
     2641 gtcgtcgtcc tgatctggcg cggcccgaag accctgccga agatcggtca ggccctcggg
     2701 cgcggcgtgc gcgaagcgcg cacggaggcc gccaagctgc gcgacggcaa gcgcgaggac
     2761 ccgcccgcct gagcgcgacc cgacccaggc gtcggctcgc cgtccgccag accgctacgc
     2821 tgacgccgtg aacgaagcca tcccgccgag cgtcgagagc ctcctcgagc ggcccggccc
     2881 cggcccgcat cggacgacga tcgtggtcga tggcgccgcc gtcgacacgc tcgccgacct
     2941 gccgccgctg cgcgaccgcc tgttgttcgt cgggctcaac ccatcgccgg tcagcgtcga
     3001 ggccggccac tacttccagg gtcgcctcgg ccagctcttc tggcgtcgcc tgatgaccgc
     3061 ttccatcctg ccgcccgaca ccgcgatcgc gtcggccgac gatgcgctcg ttgccgccgg
     3121 gcacggcctg acggatctgc tcaaggtccc caccccacgc gacgaggcgt ccgacgccac
     3181 gctccgggcg ggcgtcgggc cactctggca gaaggtcgcg atctggcgcc cggcggcgat
     3241 cgtcttcgtc tacaagcggg ccgccgagat cgtcgcggcc cgtccgctgg ccgagccgtg
     3301 gggccagctc gcaggcgtgg cgatgtcggg ccggccgtgc ttcctgatgc cggggccgta
     3361 cgcgccgcag gccgaggtgg ccgccgccct caacttcgtg cggaacctcg gggcgtcact
     3421 cccccgctga gtccggagcg atctcgagcc ggcccgagcc cggcgggccg agccaaacgc
     3481 ggagctcgac gtcggccggc agcagatccc cgtcgccgag ctcgaccgtc gggtacggga
     3541 gatgccggcc cgcccccggt gcgacgagga tcgggatctc gagagggatc gcgagcgact
     3601 gtgcggccgc cgcgtgatca ggatccgtgg ccgtcaggac gatcgcgttg atcgtgccgc
     3661 cgcgtcgctg gacggcggcg ccgatcaggt tcagcgccgc gtccgaagga tcgccgggat
     3721 cgacgaggac gagctcgcgg cgcccgtaca gggtggtcgc gccggtccga cccggcagcg
     3781 ccccgaccgc tccgaacgtg aaccggacga cggtcggcgt ttccgagtcc accctcggtg
     3841 gggccaccgg cccgagcgtg atccgatcgg cgacctccga ggatttcgat gcgccggtct
     3901 cgatgagccg ctccaggacg cacgtggtcg gcacccacat gtcgatctcg ccggcggcga
     3961 cctggtcgag ggcctgtgac ggcgtcaccc agcggtgagc ggcaacctca tcgggggcga
     4021 acgctggaac cgcctccggc ggcaggtcgg cgacgaagaa ccacgtcgag aagcgccgcg
     4081 ccatgaacgc cggcgtcgac caatgggcga tcggcgcgag ccgctcgatc ccgtcgaggg
     4141 tgacgccggc ctcttcgcgc agctcgcgca gcgccgcccg atggacggcg agcgcctcgt
     4201 tcgggggcag gttgtcgccg agcgcgcgtg acgcagctcc gaccgccccg gatgcgtgat
     4261 cggccggcgt gagcgc
//
LOCUS       DATAIW010000219         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_11317, whole genome shotgun sequence.
ACCESSION   DATAIW010000219 DATAIW010000000
VERSION     DATAIW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11317"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VHM48_06995"
     CDS             complement(<1..199)
                     /locus_tag="VHM48_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755192.1"
                     /translation="MSRTLRARLGAGLLGLALLLPLAAAPASAAAADFPAGKTGYHTY
                     AEMVADVNAVVAAHPTIAQKVA"
     gene            349..>1706
                     /locus_tag="VHM48_07000"
     CDS             349..>1706
                     /locus_tag="VHM48_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2755193.1"
                     /translation="MTTESQPRLKITYATLRADNERLHLEYEAGLAEAKTLLDGHHRN
                     FVDGRERDGEGESTVRSPIDRDMVLGHFSIGTRQDVKDAVAAARRAQPAWFALGWEKR
                     IEILKRAAELISQRQMVYGGLMAIEVGKTRLEALGEVEEAADLIRYYAKTAEDNAFYD
                     HPMDSLGDATVHTRSILRPHGVFAVISPFNFPMALAAGPTGAAMLAGNTVVFKPSSAA
                     PISGVKLVEAYRDAGVPDGVINLVMGPGETVGAELRENPGIDGIVFTGSYEVGFDLYR
                     TFSSRFPKPTIVEMGGKNPAVVSRHADLEEAAEGIMRSAFGFGGQKCSANSRVYVEKP
                     VHDELVRLLVEKTEAITIGDPLLRENWLGPIIDQRAVDRHQQASAEARRDGRVFTGGE
                     RLSDRGMDRGFYVEPTIVGNLPADHRVFRDELFAPFTAVAAVDSFDEAIRLANDSVYG
                     LTA"
BASE COUNT          273 a          621 c          578 g          234 t
ORIGIN      
        1 tcgccacctt ctgcgcgatc gtcgggtggg cggcgacaac ggcattcacg tcggcgacca
       61 tctcggcata cgtgtggtag ccggtcttgc cggccgggaa gtcggccgcg gccgcgctgg
      121 ccggagccgc ggcgagcggg aggaggaggg ccaggccgag caggccggca ccgagacgag
      181 cgcgcagggt tcgagacatg cgctcaggat gcccccgcga cgcgccgagc gccaacccac
      241 cgaacggtca cggtcgcgaa ggggaccaac gggtcgcccg catccgggcc cgcgctagac
      301 tgatcgcaac gtcccgatgc cccctccgct ccccgaggtt cccgaccgat gacgaccgag
      361 tcgcagccgc gtctcaagat cacctacgcc accctccgcg ccgacaacga gcggctccac
      421 ttggagtacg aagcgggcct cgccgaggcc aagacgctcc tcgacggcca tcacaggaac
      481 ttcgtcgatg gccgcgagcg cgacggcgag ggcgagtcga ccgtccgctc gccgatcgac
      541 cgcgacatgg tcctcggcca cttctcgatc ggcacccgcc aggacgtcaa ggacgcggtc
      601 gcggccgcac gccgcgccca gcccgcctgg ttcgcgctgg gctgggagaa gcggatcgag
      661 atcctcaagc gcgccgccga gctgatcagc cagcgccaga tggtctacgg cggcctgatg
      721 gcgatcgagg tcggcaagac ccggctcgag gcgctcggcg aggtcgagga ggcggctgac
      781 ctgatccgct actacgcgaa gacggccgag gacaacgcct tctacgacca tccgatggac
      841 agccttggcg acgcgaccgt ccacacccgt tcgatcctcc ggccgcacgg cgtgttcgcg
      901 gtgatcagcc cgttcaactt cccgatggcc ctcgccgccg gcccgaccgg ggccgcgatg
      961 ctggccggca acaccgtcgt cttcaagccg agctcggccg cgccgatctc gggtgtcaag
     1021 ctcgtcgagg cctatcgcga cgcgggcgtc cccgacggcg tgatcaacct cgtcatggga
     1081 cccggtgaga ccgtcggcgc cgagctgcgc gagaaccccg gcatcgacgg gatcgtgttc
     1141 acggggtcct acgaggtcgg cttcgacctg tatcgaacct tctcgtcgcg cttccccaag
     1201 ccgacgatcg tcgagatggg cggcaagaat cccgcggtcg tgtcgcgcca cgccgacctc
     1261 gaggaggcgg ccgaggggat catgcggtcc gcgttcgggt tcggcggcca gaagtgctcg
     1321 gccaacagcc gggtctatgt cgagaagccc gtccacgacg agctcgtccg gctcctcgtc
     1381 gagaagaccg aggcgatcac gatcggcgac ccgctcctcc gcgagaactg gctcggcccg
     1441 atcatcgatc agcgcgcggt cgaccgccac cagcaggcca gcgccgaggc acgtcgcgac
     1501 ggccgcgtct tcaccggcgg cgagcgcctg tccgatcgcg ggatggaccg cggcttctat
     1561 gtcgagccga cgatcgtcgg caacctgccc gccgaccacc gggtattccg cgacgagctg
     1621 ttcgcgccgt tcacggcggt ggccgcggtc gactcgttcg acgaggcgat ccggctcgcc
     1681 aacgactccg tctacgggct gaccgc
//
LOCUS       DATAIW010000220         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_160865, whole genome shotgun sequence.
ACCESSION   DATAIW010000220 DATAIW010000000
VERSION     DATAIW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160865"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHM48_07005"
     CDS             <1..423
                     /locus_tag="VHM48_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2755194.1"
                     /translation="ASPSLACLSALARALDVPIAWFLVDETRPPQVTRAADRRWREMP
                     GGRTSRIDARGSSDISIVELQSEPGRSTGVHTHPGDEHHLVTAGRFRMTQGGHTVELE
                     PGDYLRWDGMIPHDAEAIGDQTAAMLVIRLNRHGEPHE"
     gene            820..1989
                     /locus_tag="VHM48_07010"
     CDS             820..1989
                     /locus_tag="VHM48_07010"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX2755195.1"
                     /translation="MKVGVARESAAGERRVALVPEVLGKLTAAGLEILVEQGAGDGAA
                     IPDSAYAEAGATIVPTADLYGQSDIVLRVQKPSDSEVKGMHQGQVVIGILQPLIDPQT
                     AAALAKQGVTAISLDAIPRTLSRAQTMDALSSQANVGGYKAVLIAANEYGRYFPLLTT
                     AAGTAKPANVLILGIGVAGLQAIGTARRLGAVVSAYDVRPETAEQAESLGAKFLKLKT
                     TIDATGAGGYARELTPDERAAQQAELNEAIGGMDIVITTAQIPGRKPPVLVTADAVSR
                     MKSGSVIVDMAASALGGNCELSKAGQTITSDNGVRIVAPDNLPATMPAGASAFYARNI
                     STLLLGMIKDSNLHLDFEDEVTKATVITMDGAVIADPVKKLLEPAPAASPSGDPS"
     gene            1986..2315
                     /locus_tag="VHM48_07015"
     CDS             1986..2315
                     /locus_tag="VHM48_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX2755196.1"
                     /translation="MSPDEVRAFLTNFTVFTLAILCGFAVISKVPATLHTPLMSGANS
                     IHGIVLVGSMFIAVNATDLLSQLLSLIAVAFATMNVVGGYVVTDRMLQMFKRKPVAAP
                     KADETEG"
     gene            2320..>3062
                     /locus_tag="VHM48_07020"
     CDS             2320..>3062
                     /locus_tag="VHM48_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX2755197.1"
                     /translation="MIDINVLDPLVRIAWLVGAVGFVIGLVRMNSPATARNGNLMSAG
                     GMAVAIAATALYLLLRPSSATGNNLLIGWLIIIVGIAIGGGLGLYTARTVKMTAMPQL
                     VSLFNAVGGGAAALIAIDGYLRISSAPGLQENVAAVLDIVIGSITFTGSLIASGKLMG
                     RIPGKPIMIPGGQLVTGGLALVVVVGAIFLWLGNYSLAAMALILAASLVFGVTMTLPI
                     GGADMPVVISLLNSFTGTAVAMAGFVLENP"
BASE COUNT          478 a         1137 c          980 g          467 t
ORIGIN      
        1 gcgtctccgt cgctcgcctg cctgtcggcg ctcgcccgcg ccctcgacgt cccgatcgcc
       61 tggttcctcg tcgatgagac gcggccaccg caggtcacgc gcgcggcgga tcgacgctgg
      121 cgcgagatgc ccggcggccg caccagccgg atcgacgccc ggggctccag cgacatctcg
      181 atcgtcgagc tccagtcgga gccgggtcgc tcgacggggg tccacacgca tcccggcgac
      241 gagcaccacc tcgtgaccgc cggccggttc cggatgaccc agggcggcca taccgtcgag
      301 ctcgagccag gcgactacct ccgctgggac ggcatgatcc cgcacgacgc ggaggcgatc
      361 ggcgaccaga ccgcggcgat gctggtcatc cgcctgaacc gtcacggcga accgcacgaa
      421 tgaagaggac gcccaggcac cgtccggctc gaggccgatg ttcattggac gaacctacgc
      481 gacgagcccg gaggtcgacg ggcatccggc gcgccgttct cgttcatcca gtgcacgaac
      541 ccagccgatg acgccagttc gcgggtctga gcgaccgctc gagggcccgg ttgctgctgg
      601 tcgtgctcca tcggttcatc caatgcacca actcgtcgat tccgttcatc cgatgcacca
      661 aacggacgag tccggccacg gcacgggtcc cggacgcggc ccctccctcg ccgccaccga
      721 atcgcaacgt cgaccggcca cgcgctcgtc tcgctgtcgg tatccttcgg acgtccccat
      781 gcaagcccgc acatcgatcg acaacgtcag gtggtgccca tgaaagtcgg cgtcgccagg
      841 gagtccgcgg ccggagaacg gcgcgtggcc ctggtacccg aagtgctcgg caagttgacg
      901 gccgccgggc tcgagattct cgtcgaacag ggcgccggcg acggcgccgc gatccccgac
      961 tccgcgtatg ccgaggcggg tgccacgatc gtcccgaccg cggacctcta cggccagtcg
     1021 gacatcgtcc tccgcgtcca gaagccctcg gactccgagg tcaaggggat gcaccagggc
     1081 caggtggtca tcggcatcct ccagccgctg atcgatccgc agaccgcagc ggccctcgcg
     1141 aagcagggcg tgaccgcgat cagcctcgac gcgatcccgc ggaccttgtc gcgggcccag
     1201 acgatggatg ccctgagctc gcaggccaat gtcggcggct acaaggcggt cctgatcgcg
     1261 gccaacgagt acggccgcta cttcccgctc ctgacgaccg ccgcgggcac cgccaagccc
     1321 gccaacgtcc tgatcctcgg gatcggcgtg gccggtctcc aggcgatcgg gacagcccgc
     1381 cgcctcggcg ccgtggtctc cgcctacgac gtccggcccg agacggccga gcaggccgag
     1441 tcgctcgggg ccaagttcct gaagctcaag acgacgatcg acgcgaccgg cgccggcggc
     1501 tacgcccgcg agctgacccc cgacgagcgg gccgcccagc aggccgagct caacgaggcc
     1561 atcggcggaa tggacatcgt catcacgacg gcccagatcc ccggtcgcaa gccaccggtc
     1621 ctcgtgaccg ccgatgcggt cagccggatg aagtcgggct cggtcatcgt cgacatggcg
     1681 gcctccgccc tcggcggcaa ctgcgagctg tcgaaggccg gtcagacgat cacctccgac
     1741 aacggcgtcc ggatcgtcgc gccggacaac ctgccggcga cgatgccggc cggcgcctcc
     1801 gcgttctacg cccgcaacat ctcgaccctc ctgctcggga tgatcaagga cagcaacctc
     1861 catctcgatt tcgaggacga ggtcacgaag gcgactgtca tcacgatgga cggcgcggtc
     1921 atcgccgacc ccgtcaagaa gctgctcgag cccgcgccgg cggcgagccc ctccggagac
     1981 ccctcgtgag ccccgacgaa gtccgcgcct tcctcaccaa cttcaccgtc ttcaccctcg
     2041 cgatcctgtg cggcttcgcc gtcatctcga aggtccccgc gacgctccac accccgctga
     2101 tgtccggcgc gaactcgatc cacgggatcg tcctcgtcgg ctcgatgttc atcgcggtca
     2161 acgccaccga cctgctcagc cagctcctgt cgctgattgc cgtcgccttc gcgacgatga
     2221 acgtcgtcgg cggctacgtg gtcaccgacc ggatgctcca gatgttcaag cgcaagcccg
     2281 tcgcggcccc gaaggccgac gagacggagg gctgaggcga tgatcgacat caacgtcctc
     2341 gacccgctcg tcaggatcgc ctggctggtc ggcgccgtcg gattcgtcat cggcctcgtc
     2401 cggatgaact cgccagccac cgctcgcaac ggcaacctca tgtcggccgg cggcatggcg
     2461 gtcgccatcg cggcgacggc cctgtacctg ctgctgaggc cgtccagcgc gaccggcaac
     2521 aacctcctga tcggctggct gatcatcatc gtcgggatcg ccatcggcgg cgggctcggg
     2581 ctgtacaccg cccggaccgt gaagatgacc gcgatgccgc agctggtgtc gctgttcaac
     2641 gcggtcggcg gcggagccgc ggccctgatc gcgatcgacg gctacctgcg gatcagctcg
     2701 gcgcccgggc tccaggagaa cgtcgcggcg gtcctcgaca tcgtcatcgg ctcgatcacc
     2761 ttcaccggct cgctcatcgc gtcgggcaag ctgatgggcc ggatccccgg caagccgatc
     2821 atgatccccg gcggtcagct cgtcacgggt ggcctggccc tcgttgtggt cgtcggtgcg
     2881 atcttcctct ggctcggcaa ctacagcctc gcggcgatgg ccctgatcct ggcggcgtcg
     2941 ctcgtgttcg gcgtgacgat gacgctgccg atcggtggcg cggacatgcc ggtcgtcatc
     3001 tcgctcctga actcgttcac cgggacggcc gttgcgatgg ccggcttcgt cctcgagaac
     3061 cc
//
LOCUS       DATAIW010000221         9448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_101149, whole genome shotgun sequence.
ACCESSION   DATAIW010000221 DATAIW010000000
VERSION     DATAIW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101149"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..2145
                     /locus_tag="VHM48_07025"
     CDS             34..2145
                     /locus_tag="VHM48_07025"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEX2755198.1"
                     /translation="MNSAAFDEFGRMTANLGLEAVPATPAGQNILLYPYIAPPTEVID
                     GTNLPKGDVNIAPIASGTDGTQIWKITHNGVDTHPIHFHLFNVQLLNRVTWDNIIIPT
                     EPSELGWKETIRVSPLEDTIVAVRPITPTLPFEVPNSIRPLSPMMPLGANLDPTGIMV
                     GPNGQLTTITNALVNFGWEYVYHCHILSHEEMDMMRPVLLALPPIAPGGLVFNQTSLT
                     SGTLTFNDNSFTETSFLVQKSLDGTTWTDVPDPTGAMNSPLDQPNVHGPRSLDDTAYD
                     PNVATSYRVVARNTVGATDALLAGGYSGTTVQSVSPALLVGVLTPTTTTLTSDVNPST
                     FGQNVTFTATVAATTGIPTGTVQFSIGGTNVGAPVALNASGVATLATSTLSIASHSVE
                     AVYFGSTFYDTSLGTLNQVVNKAPSTTALTSSLNPSFFGQSVTFTATVGPATATGTVV
                     FNINGLDGAPVALVAGKATFTTTTLPAGDNAVIATYLGDANYLTSNATLTQVVSPLIA
                     TTTTLTSSPNPSVFGQSVTFTATVSPVAATGTVVLTIDGVAGAPIALNASGVATFATT
                     ALTVGNHAVVAMYGGNATYATSASTTLTQVVNKATTTTTVRSSDTNGSVGDTIRLTAT
                     TTAVAPGAGTRTGTVEFKISNGAGIKVLAAAVPVPANGVTVFSWTLLASQVGTWTVTA
                     TYSGDANFAGSLGTLANQRVR"
     gene            2837..4498
                     /locus_tag="VHM48_07030"
     CDS             2837..4498
                     /locus_tag="VHM48_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX2755199.1"
                     /translation="MSTTDPLSLTEAKRLAGNADTLLLRATRPADLETPIGAFLRLDD
                     GTTPAYLLESVEGGERLGRYSFLGVGPRRLLEVRDGIARTQTRPVTVESYSPDLPVDV
                     AEAPDPLEAIRRFVPRRRVVPTEGMPRFTGGAVGALAYDAVTAFEPTVALPDRDPVGV
                     PTAAFIETDLVLVFDHLTHTLSAIASLHTETSDLEGRYRIAEQAIFEVLERTARPSAA
                     EIAGPGRPVENGDAGPAAGSGGLREDAIETSLGRDAYIHAVEVAKEAIASGEAIQVVL
                     ARRQSFELPTDPATGRPLDGISLYRALRRVNPSPYLFFVRTPLFEVVGASPELLLQVE
                     GDKLTTHPIAGTRPRGADEREDAILAEQLQRDPKERAEHIMLVDLGRNDLGRVSRPGT
                     VTVSKYMEVEKYSHVLHLVSHVEGRLKPELDALDALRSVFPAGTLSGAPKVRAMQLIA
                     AAEGERRGLYGGAVGYLGYDGNLDTAITIRSAVLRKGQAHIHTGAGIVAGSVPEKEFE
                     ETEHKAAALRRAIELAAAPADAPRTRSDQPVGAAPAGVATAGDGR"
     gene            4495..5211
                     /locus_tag="VHM48_07035"
     CDS             4495..5211
                     /locus_tag="VHM48_07035"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX2755200.1"
                     /translation="MILVIDNYDSFTFNLVQALQAAGADVRVVRNDAIDRAGVEALAD
                     DPGVDLRGIVVSPGPGDPSSAGVSVATIEVARDRAIPLLGVCLGMQSMAVAFGASIVR
                     APTLVHGEASEVTHDGAGLLAGMPPAFMAARYHSLAVDVATLPAELRVTAMSEVDRVI
                     MGIRHVSLPLEGVQFHPESVLTPEGPHLLANFLRQAGEGEASRLDDATGSFATAGLAS
                     APGGAPMASTSANLPAEAVR"
     gene            5208..6260
                     /gene="trpD"
                     /locus_tag="VHM48_07040"
     CDS             5208..6260
                     /gene="trpD"
                     /locus_tag="VHM48_07040"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX2755201.1"
                     /translation="MSDEVRTALSAVVDGRTLTLDEARDAMGSVMDGLATPALLAALL
                     VALRMRGETVEELAGFATAMRERALRVDAPPDAIDVVGTGGDQSGTFNVSTTAALVVA
                     ASGVPVAKHGNRAITSRAGSADVLDTLGVRIDHDPLSAGVALREIGFAFMFAPGFHPA
                     MKHAGPTRREIGVRTAFNLLGPLTNPARVSRLLVGTGDPAAAPKLAEVLRILGAERAF
                     VVHGTGVDELPLDGTGVLYDITPAGVERRTVDAAALGLGRVATTKLAGGTPAENATIV
                     ESILRGEPGARRDVVLLNAGAALMVAGAVDDLAAGIDRAALTIDAGLATQLLAALRAE
                     RRAAEAARPVVEGAPA"
     gene            6257..9157
                     /gene="trpB"
                     /locus_tag="VHM48_07045"
     CDS             6257..9157
                     /gene="trpB"
                     /locus_tag="VHM48_07045"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX2755202.1"
                     /translation="MTVDDRPELDRLPPPDDDTPDPADAAAAGTRNVVEEIAERRRAD
                     IRDEMARLTLDEHLAIAEATPAPRPILDRLAAPGLHLIAEIKRSSPSAGEIAAPDEDI
                     VARARAYEAGGASAISVLCEPHWFGGSVEDLRRVRAAVSIPVLAKEFVVEEIQLPHLR
                     AAGADLVLLLAVLHPPRRLADLVERALEIGLEPLVEAHDEEELKAALATKTRIIGINN
                     RDLRTLAVDTGQAIRLRSLVPDDRLVVAESGVRDAATVAAWRAVGFDAALVGEALVRA
                     GDPAAAARAFVSAGRLPTDPANRAATAFVKVCGITDAEGVLAAVRSGADAIGLNLVPG
                     TPRELTLAEAIPLARIARSLAANPPRIVAITADADRDRIAEIVAALDPDAVQFNGDET
                     TDDLAAAGRAAWKALRVASNDDAATVVGRAREFLDAGAERILLDAAGGPHPGGTGIRI
                     ATSLAAAVAREVPILLAGGLDPANVAGALREVPALGVDSASGTERPPVVGQRRTKDPF
                     RVALFVKRAHAARLDRPNLAVRPTPVPAGLLTADAAGRWGLERQFGGRYVPETLMAAL
                     EQLEDAYQAVCHDPVFWSELRELLARYAGRPTPLYRADRLAYEALERAVALDPTARLP
                     HRLRLYLKREDLAHTGAHKINNALGQALLTRRLGKERVIAETGAGQHGVATATACALL
                     GLPCVVFMGAVDIERQQPNVLRMRALGAEVRPVTSGTATLKDAVNDAMRDWVTNVETT
                     HYVLGSAMGPHPYPTIVRDLQRRIGDEAAAQVAEVEGRLPDLALACVGGGSNAIGLLD
                     RFIGEPSVRLAVAEAAGDGIETGKHAAALAGGTPGILHGSRSMMLQDADGQVLEAHSA
                     SAGLDYPGVGPQIAALAAAGRLEVQAATDGEAFEAMRWLSRTEGILPALESAHALAVL
                     PRILAGAGAGASGGAAHLPGAWPEEAVVLVGLSGRGDKDLSQFGRWIEEHEA"
     gene            9154..>9448
                     /locus_tag="VHM48_07050"
     CDS             9154..>9448
                     /locus_tag="VHM48_07050"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2755203.1"
                     /translation="MSAIPASVGGAALPTGTNDTTGAHRIAAAFAAARSAGRIALIPY
                     VVAGYPDAAAGLRIAIAAIDAGADLLEVGLPYSDPLADGATLQRASQVALAAGA"
BASE COUNT         1398 a         3544 c         3207 g         1299 t
ORIGIN      
        1 gtcctgatcg agccgaaggc gctccacgac gagatgaact cggccgcctt cgacgagttc
       61 ggacggatga cggccaacct cggcctcgag gccgtgcccg ccacgcccgc cgggcagaac
      121 atcctgctct acccgtacat cgcgcctcca accgaggtca tcgacgggac caacctgccg
      181 aagggcgacg tcaacatcgc gccgatcgcg tcggggaccg acggcaccca gatctggaag
      241 atcacgcaca acggcgtgga cacccacccg atccacttcc acctgttcaa cgtgcagctg
      301 ctcaaccggg tgacgtggga caacatcatc atccccaccg agccgtcgga gctgggctgg
      361 aaggaaacga tccgcgtcag cccgctcgag gacacgatcg tcgccgtgcg accgatcacc
      421 ccgacgctgc cgttcgaggt cccgaacagc atccggccac tgagtccgat gatgccgctc
      481 ggcgcgaacc ttgacccaac cgggatcatg gttggaccga acggccagct cacgaccatc
      541 accaacgcgc tcgtcaactt cggttgggag tacgtgtatc actgccacat cctcagccat
      601 gaggagatgg acatgatgcg gcctgtgctg ctcgcactgc cgccgattgc cccaggtggc
      661 ctggtcttca accagacgag cctgacctcg gggacgttga ccttcaacga caactcgttc
      721 accgagacgt cgttcctcgt tcagaagtct ctcgacggca caacgtggac cgatgttccc
      781 gacccaacgg gggccatgaa ctcgccactt gatcagccga atgttcacgg accccggtcg
      841 ctggacgaca cggcctacga tccgaatgtt gcgacctcct accgagtcgt ggcccgaaac
      901 acggtcggcg caaccgacgc gctcctcgcg ggcgggtatt cgggcacgac cgtgcagtcc
      961 gtctcgcccg cgctgctcgt tggtgtgctg acgcccacca cgacaacgct cacgagcgac
     1021 gtcaacccat cgactttcgg gcagaacgtg accttcacgg ccacggtcgc tgccaccaca
     1081 gggattccga ccgggacggt gcagttcagc atcggcggta ccaacgtcgg cgcacccgtc
     1141 gcgctcaacg cctctggcgt ggcgacgctt gcgaccagca cgctgtccat tgccagccac
     1201 tccgtggaag ccgtctactt tgggtccacc ttctacgaca cgagccttgg aacgctgaat
     1261 caggtcgtga acaaggcgcc ctcgacaact gccctcacca gtagcctcaa cccgtcgttc
     1321 ttcggccaga gcgtgacctt cacggccacg gtcggaccgg cgacggccac gggtacggtc
     1381 gtgttcaaca tcaacggtct tgatggcgct ccggtggcac ttgtcgccgg gaaggctacc
     1441 ttcacgacca ccacccttcc ggctggcgac aacgccgtga tcgcgaccta ccttggcgat
     1501 gcgaactacc tgaccagcaa cgcgacgctg acacaggtcg tcagcccgtt gatcgctacg
     1561 acaaccaccc tgaccagcag ccccaacccg tcggtcttcg gacagagcgt gaccttcacg
     1621 gccacggtca gcccggtggc cgctacggga acggtcgtgc tcaccatcga cggcgtcgct
     1681 ggtgctccga tcgccctcaa cgcatccggt gtggcgacgt tcgccaccac agctctgacc
     1741 gtaggtaacc acgccgtggt tgccatgtat ggcggcaacg ccacctacgc gaccagcgct
     1801 agcacgacgc tgacgcaggt cgtgaacaag gcgacgacca cgaccacggt caggagcagc
     1861 gacaccaacg gtagtgtcgg tgacacgatc cgcctcacgg ccaccaccac cgcggtcgca
     1921 ccgggagccg gaacgcgcac cggcaccgtc gagttcaaga tcagcaacgg cgcgggcatc
     1981 aaggtccttg ctgctgctgt cccagttccg gcgaacggcg tcaccgtctt cagctggacg
     2041 ttgctcgcct cgcaggttgg gacgtggacc gtcacggcca cgtatagcgg cgacgccaac
     2101 ttcgccggaa gccttggcac tctcgccaac cagcgagtga ggtagctacc ccacaacccg
     2161 tagcagaccc ctatcccccg gcgccggttc cccccaccgg cgccggggag ccacccggag
     2221 ccctcggctc cgtccgtcaa ccattagaac accgagatga cgtgcccatc ctcgccagcc
     2281 tggagtcacc cggcccgcct gggccgggtg acgggccatc cgtgaccggc ggcgcacaag
     2341 ctgatcgtct ccctcgtctt ctcgatgaga tcgagagtga caaagtcgct gtccccttgg
     2401 ctcggacaga atcgctgtcc ttcgacacca tccagatacg tccttgacgc ccgggagcac
     2461 ggaacctaga ctccgccgac atcacgaaga acgggctgtg aacgggacga gtccccgtcg
     2521 ggcgtcggca gagagatccg gtggatggtg gaaaccggag cgacgacggc ggcaaatgga
     2581 cccgggagcc tccggaccga acggcgagcg gcgcggcgac gcggagcgga cccagtaggc
     2641 tccggcgcag agcgccagcg atagagggca gcgccgatcg gcaggaccgc gcaagcggcc
     2701 acccgcccgt cggtcatgag agtgcggcat cgccgaatga gggtggcacc gcggaaggca
     2761 cgacctttcg tcccgggacg aattggtcgt ttcttgattc tcgggcgcac gaacgagccc
     2821 cggaggccgc aacacgatga gcaccaccga tccgctcagc ctgaccgagg cgaagcgcct
     2881 ggccggcaac gccgacacat tgctcctgcg cgccacccgc ccggccgacc tcgagacccc
     2941 gatcggcgcc ttcctccgcc tcgatgatgg caccacgccg gcctacctcc ttgaatcggt
     3001 cgagggcggc gagcgcctcg ggcggtactc gttcctcggc gtcggtccgc gccgactgct
     3061 cgaggtccgc gacgggatcg cccggacgca gacacggccg gtgaccgtcg agagctacag
     3121 cccggacctg ccggtcgacg ttgccgaggc gcccgatccg ctcgaagcga tccgccgctt
     3181 cgtcccgcgc cggcgggtcg tgccgaccga ggggatgccg cgcttcacgg gcggcgcggt
     3241 cggcgccctt gcctacgacg cggtgaccgc cttcgagccg accgtcgcac tccccgatcg
     3301 cgatcccgtc ggcgtcccga ccgcggcatt catcgagacc gacctcgtcc tcgtgttcga
     3361 ccacctgacc cacaccctga gcgcgatcgc gtcgctccac accgagacgt ccgatctcga
     3421 gggtcgctac cggatcgccg agcaggcgat cttcgaggtg ctcgagcgga ccgcccggcc
     3481 gagtgccgcc gagatcgccg gaccggggcg accggtcgag aacggcgacg cgggtccggc
     3541 agcggggtcc ggcggcctcc gcgaggacgc gatcgagacg agcctcggcc gggacgccta
     3601 catccatgcc gtcgaggtcg ccaaggaggc catcgcgtcg ggagaagcga tccaggtcgt
     3661 gctcgcccgg cgccagtcgt ttgagctgcc gaccgacccc gcgaccggcc ggccgctcga
     3721 cgggatctcg ctctaccgcg ccctccgccg ggtcaacccg agcccgtacc tgttcttcgt
     3781 ccggacgccg ttgttcgagg tcgtcggagc cagcccggag ctcctcctcc aggtcgaggg
     3841 cgacaagctc acgacccacc cgatcgcggg cacccggccg cgcggggccg acgagcgcga
     3901 ggacgcgatc ctcgccgagc agctccagcg cgatccgaag gagcgggccg agcacatcat
     3961 gctcgtcgac ctcggccgga acgacctcgg ccgggtgtcg cggcccggga cggtgaccgt
     4021 gagcaagtac atggaggtcg agaagtacag ccacgtcctt catctcgtgt cccatgtcga
     4081 gggccggctc aagccggagc tggatgcgct cgatgcgctc cgctcggtct tcccggccgg
     4141 gaccctgagc ggcgcgccga aggtccgggc gatgcagctg atcgcagccg cggagggcga
     4201 gcggcgcggg ctgtatggcg gcgcggtcgg ctacctcggc tacgacggca acctcgacac
     4261 ggcgatcacg atccggagcg ccgtcctgcg caagggccag gcccacatcc acaccggcgc
     4321 cgggatcgtc gccgggagcg tgccggagaa ggagttcgag gagaccgagc acaaggcggc
     4381 ggccctccgc cgggcgatcg agctggcggc cgcacccgcc gatgccccgc gaacgcggtc
     4441 tgaccagccg gtcggagcgg cgccggctgg agtggcgacg gccggggacg ggcgatgatc
     4501 ctcgtcatcg acaactacga cagcttcacg ttcaaccttg tccaggccct ccaggcggcc
     4561 ggcgccgacg tccgggtcgt tcgcaacgac gcgatcgacc gggccggggt cgaggccctc
     4621 gccgatgacc ccggcgtgga cctccgcggg atcgtcgtct cgccgggccc gggcgacccg
     4681 agctcggccg gtgtctcggt cgcgacgatc gaggtcgccc gcgaccgggc aatcccgctc
     4741 cttggcgtgt gcctcgggat gcagtcgatg gcggtcgcgt tcggggcctc gatcgtgcgc
     4801 gccccgaccc tcgtccacgg cgaggcgtcc gaggtgaccc acgacggtgc cgggctgctg
     4861 gccgggatgc cgccggcgtt catggcggcg cgctaccact cgctggcggt cgacgtcgcg
     4921 acgctcccgg cggagctccg ggtgaccgcg atgagcgagg tcgaccgggt catcatgggg
     4981 atccgccacg tctcgctgcc cctcgagggc gtccagttcc atcccgagtc ggtcctgacc
     5041 ccggagggcc cgcatctcct cgccaacttc ctgcgccagg ccggcgaggg cgaggcgtca
     5101 cgtctcgatg acgcgacggg ctcgttcgcg acggcgggtc tcgcctcggc tccgggcggc
     5161 gccccgatgg catccacctc ggccaatctg ccagccgagg cggtccgatg agcgacgagg
     5221 tccggacggc gctgagcgcg gtcgtcgatg gccggacgct gaccctcgac gaagcccgcg
     5281 acgcgatggg ctcggtcatg gacggcctgg cgacaccggc cctcctggcg gccctcctcg
     5341 tcgccctccg gatgcgcggc gagacggtcg aggagctggc gggcttcgcg accgcgatgc
     5401 gcgagcgcgc cctccgggtc gatgcgccgc ccgacgcgat cgacgtcgtc ggcaccggcg
     5461 gcgaccagag cgggaccttc aacgtgtcga ccaccgccgc tctcgtcgtc gccgcgagcg
     5521 gcgtcccggt cgccaagcac ggcaaccggg cgatcacatc ccgcgccggc tcggccgacg
     5581 tcctcgacac cctcggcgtc cggatcgacc acgacccgct gtcggccggg gttgctctgc
     5641 gcgagatcgg attcgccttc atgttcgccc cgggcttcca cccggcgatg aagcacgccg
     5701 gcccgacccg ccgcgagatc ggcgtccgga ccgcgttcaa cctgctcggc ccgctcacga
     5761 acccggcccg ggtgtcgcgc ctcctggtcg ggaccggcga tcccgctgcc gcgccgaagc
     5821 tcgccgaggt cctccggatt ctcggcgcgg agcgtgcctt cgtcgtccac ggaaccgggg
     5881 tcgacgagct gccgctcgac gggacggggg tcctctacga catcacgccc gccggcgtcg
     5941 agcggcggac cgtcgatgcc gctgcgctcg gcctgggccg ggtcgccacg acgaagctcg
     6001 ccggcgggac gccggccgag aacgcgacga tcgtcgagtc gatcctccgc ggcgaacccg
     6061 gcgcacggcg ggacgtcgtc ctcctgaatg ctggcgccgc cctcatggtc gccggtgccg
     6121 tcgacgacct ggccgccggg atcgaccggg ccgcgctgac gatcgacgca ggtctggcca
     6181 cgcagcttct cgctgcgctc cgcgccgagc gacgggctgc cgaggcggct cgcccggtgg
     6241 tcgaaggagc gccggcatga cggtcgacga tcgccccgag ctcgatcgat tgccgccgcc
     6301 ggacgacgac acgcccgatc cggccgacgc tgccgcggcc gggacccgca acgtcgtcga
     6361 ggagatcgcc gagcgccgcc gcgccgacat ccgcgacgag atggctcgcc tgacgctcga
     6421 cgagcacctc gcgatcgcgg aggccacacc agccccgcgc ccgattctcg atcggctggc
     6481 cgcgcccggc ctccacctga tcgcggagat caagcgatca tcgccgtcgg ccggcgagat
     6541 cgcggcgccg gacgaggaca tcgttgcccg ggcccgcgcc tacgaggccg gtggcgccag
     6601 cgcgatctcg gtgctgtgcg agccgcactg gttcggcggc tcggtcgagg acctccgccg
     6661 ggtgcgggcg gccgtctcga tcccggttct cgccaaggag ttcgtcgtcg aggagatcca
     6721 gctgccccac ctccgggccg ccggcgcgga tctcgtcctc ctgctcgcgg tccttcatcc
     6781 gccgaggcgc ctcgccgacc tcgtggagcg tgcgctcgag atcggcctcg agccgctggt
     6841 cgaggcccac gacgaggagg agctcaaggc ggccctcgcg acgaagacgc ggatcatcgg
     6901 gatcaacaac cgcgacctgc ggacgctcgc ggtcgacacc ggtcaggcga tccggctgcg
     6961 ttcgcttgtg cccgacgacc gcctcgtcgt cgccgagtcc ggggtccgcg acgccgccac
     7021 ggtcgcggcc tggcgggcgg tcgggttcga cgccgcgctc gtcggcgagg ccctggtccg
     7081 ggccggcgat ccggccgcgg cagcccgcgc gttcgtgtcc gccggccgcc tgccgaccga
     7141 ccccgcgaac cgcgcggcca cggcgttcgt gaaggtctgc gggatcaccg acgcggaggg
     7201 cgtcctggcc gcggtccgca gcggcgccga cgcgatcggg ctgaacctcg tgccgggcac
     7261 cccgcgtgag ctcaccctcg ccgaggcgat cccgctggcc cggatcgccc gctcgctggc
     7321 ggccaacccg ccgcggatcg tcgcgatcac cgccgacgcg gatcgcgacc ggattgccga
     7381 gatcgtggcc gccctcgatc cggacgcggt ccagttcaac ggcgacgaga cgaccgatga
     7441 cctcgccgcg gccggacgag cggcctggaa ggctctccgg gtggcgagca acgacgatgc
     7501 cgcaacggtc gtgggccggg cgcgcgagtt cctcgacgcc ggcgccgagc ggatcctcct
     7561 cgacgccgcc ggcggtccac acccgggcgg caccgggatc cggatcgcca cgagcctcgc
     7621 tgccgcggtt gcgcgcgagg tgccgatcct cctcgcgggt gggctggatc cggccaacgt
     7681 cgccggtgcc ctgcgcgagg tccccgcgct cggggtcgac tcggcctcgg gcaccgaacg
     7741 cccgccggtc gtcggccagc gccggacgaa ggacccgttc cgcgtcgccc tgttcgtcaa
     7801 gcgggcgcac gccgcccgcc tcgaccgccc gaatctcgcg gtccgcccga caccggttcc
     7861 ggccggcctc ctcaccgccg acgccgccgg tcgctggggc ttggagcgcc aattcggcgg
     7921 ccgctacgtt cccgagaccc tgatggcggc cctcgaacag ctggaggacg cgtaccaggc
     7981 cgtttgccac gatcctgtct tctggtccga gctccgcgag ctcctggccc gctacgccgg
     8041 ccgcccgacc ccgctctacc gcgccgaccg cctcgcctac gaggccctcg agcgggcggt
     8101 cgccctcgac ccgacggctc gtctcccgca tcgcctccgg ctctacctga agcgcgagga
     8161 cctcgcccat accggggccc acaagatcaa caacgccctc ggccaggcgc tcctcacccg
     8221 gcggctcggc aaggagcgcg tcatcgccga gacgggcgcc ggccagcacg gcgtggccac
     8281 cgcgacagcg tgtgccctgc tcgggctgcc atgcgtcgtc ttcatgggcg cagtcgacat
     8341 cgagcgccag cagccgaatg tcctgcggat gcgggcgctc ggggccgagg tccggcccgt
     8401 gacgtccggt accgcgaccc tcaaggatgc cgtcaacgac gcgatgcgcg actgggtgac
     8461 gaacgtcgag acgacccact acgtcctggg atcggcgatg ggcccgcacc cctacccgac
     8521 gatcgtccgc gacctccagc ggcggatcgg cgacgaggcg gcggcccagg tcgccgaggt
     8581 cgagggacgg ctgcccgacc ttgccctggc ctgcgtcggc ggcggctcga acgcgatcgg
     8641 gctgctcgac cggttcatcg gcgagccgtc ggtgcggttg gccgtggccg aggcggctgg
     8701 cgacgggatc gagaccggga agcacgcggc tgcgctcgcc ggaggcacgc ccgggatcct
     8761 gcacgggtcg cgctcgatga tgctccagga tgccgacggc caggtgctcg aggcgcactc
     8821 ggcctcggcc gggctcgact acccgggcgt cggtccgcag atcgctgcgc tcgccgcggc
     8881 gggccggctc gaggtccagg ccgcgaccga cggcgaggcg ttcgaggcga tgcgctggct
     8941 gtcgcggacc gaggggatcc tgccggccct cgagagcgcc cacgcgctcg cggtgctgcc
     9001 gcggatcctg gccggcgccg gcgccggcgc gagcggcggt gccgcgcact tgcccggcgc
     9061 ctggcccgag gaggcagtcg tgctcgtcgg gctgtccggg cgcggcgaca aggacctgtc
     9121 ccaattcggc cgctggatcg aggagcacga ggcgtgagcg cgattcccgc gtcggtgggc
     9181 ggcgccgctc tgccgaccgg cacgaacgac acgaccggcg cccaccggat cgccgccgcc
     9241 ttcgccgcgg ctcgcagcgc cggccggatc gccctgatcc cgtacgtcgt ggccggctat
     9301 ccggacgccg ccgcgggcct gcggatcgcc atcgccgcga tcgacgccgg ggcggacctc
     9361 ctcgaggtcg gcctgccgta ttcggatccg ctcgccgacg gcgccaccct ccagcgggcc
     9421 tcacaggtcg cgctcgcggc gggggcga
//
LOCUS       DATAIW010000222         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_41862, whole genome shotgun sequence.
ACCESSION   DATAIW010000222 DATAIW010000000
VERSION     DATAIW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41862"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VHM48_07055"
     CDS             <1..600
                     /locus_tag="VHM48_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2755204.1"
                     /translation="AVGAVPIADEVQVGFGRVGSAVWAFEAQGARPDIVTMGKPIGNG
                     HPLGAVVTTRAIADAFNNGMEYFNTFGGNPVSAAIGLAVLDVIADEGLQEHARFTGER
                     LLAGLREVAARHEPIGEVRGMGLFVGFELVRDRATREPDAALATELVNRAAERGVLLS
                     TDGPDHDVIKIKPPMVFSAADADRLVETVDTVLTEVGSS"
     gene            complement(620..1195)
                     /locus_tag="VHM48_07060"
     CDS             complement(620..1195)
                     /locus_tag="VHM48_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX2755205.1"
                     /translation="MFRRFLGLDRQPDPRLPVGDTAAPLPDSAAETATARHNPARLEA
                     LPPDEARFLACFAYVMSRAANADFDISAEETALMERFAVEHGGLDTAQAVLVVEMAKL
                     QARVNGGTEDFVVTREFRSISTPAQRLDLLRCCFAIGAADGTISAAEAGTINEIAREL
                     DLELNDVNAARAEFHEQLSAVQALRRDVGGS"
     gene            complement(1207..1689)
                     /locus_tag="VHM48_07065"
     CDS             complement(1207..1689)
                     /locus_tag="VHM48_07065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2755206.1"
                     /translation="MSADATPSLQQRYAPRGRCFGCGPANPIGLHVESRPDPDDPGVL
                     VASWTPLADHEAFAGVVNGGILGTLFDCHANWTATWHLMRARGADRPPTTVTLDYAIR
                     MRRPTPSDRPVDLRAWVVEAGEDRATVEAEIASGGVVTATGRATFVAVKPDHPAYDRW
                     "
     gene            1823..>2167
                     /gene="lepB"
                     /locus_tag="VHM48_07070"
     CDS             1823..>2167
                     /gene="lepB"
                     /locus_tag="VHM48_07070"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2755207.1"
                     /translation="MDHETRETPAPEGATRGEKTGHRGGRLGCAVEIVETLVLTLVIF
                     VVIQNFVAQPFKVEGSSMERTFVESDYVLVDRLTPRWSPYARGQVVVFQPPETVSDRK
                     EPFIKRVIGVGGD"
BASE COUNT          306 a          741 c          799 g          321 t
ORIGIN      
        1 gctgtcggcg cggtcccgat cgccgacgag gtccaggtcg ggttcgggcg ggtcggttcg
       61 gccgtatggg cgttcgaggc gcagggcgcc cgtccggaca tcgtcacgat gggcaagccg
      121 atcggcaacg gccatcccct cggcgcggtc gtcacgaccc gcgcgattgc cgacgcgttc
      181 aacaacggga tggagtactt caacacgttc ggcggcaatc ccgtgtcggc cgcgatcggg
      241 ctcgccgtcc tcgacgtcat cgccgacgag gggctccagg agcacgctcg gttcaccggg
      301 gagcggctgc tcgccggcct ccgcgaggtc gccgcccggc acgagccaat cggcgaggtc
      361 cgcgggatgg gcctcttcgt cgggttcgag ctggtccgcg accgcgccac ccgcgagccc
      421 gacgccgcgc tcgcgaccga gctcgtcaac cgcgccgccg agcgcggcgt cctcctcagc
      481 accgacgggc ccgaccacga cgtgatcaag atcaaaccgc cgatggtctt ctcggccgcc
      541 gacgccgatc gcctcgtcga gacggtcgac accgtgctca ccgaggtcgg gtcgtcctga
      601 tcgaccaggc cgcggcgcgt cagctcccgc cgacatcgcg gcgcagcgcc tggaccgccg
      661 acagctgctc gtggaactcg gcccgggcgg cgttgacgtc attcagctcg agatcgagct
      721 cccgggcgat ctcgttgatc gtcccggctt cggcggcgct gatcgtgccg tccgccgccc
      781 cgatcgcgaa gcagcagcgc aacaggtcga gccgctgggc gggggtcgag atcgagcgga
      841 actcgcgggt caccacgaag tcctccgtgc cgccgttgac ccgggcctgg agcttggcca
      901 tctccacgac gaggacggct tgggcggtgt cgagcccgcc gtgctcgacc gcgaagcgct
      961 ccatcagggc ggtctcctcg gcgctgatgt cgaagtcggc gttggcggcc cggctcatca
     1021 cgtaggcgaa gcaggcgagg aagcgggcct cgtccggcgg gagggcttcc aggcgcgccg
     1081 gattgtggcg cgcggtcgcg gtctcggcgg cggaatcggg caggggtgcg gcggtgtcgc
     1141 cgaccggcag acgcggatcc ggttggcgat cgaggcccag gaatcgtcgg aacatggagg
     1201 gatctcctac cagcggtcgt atgcgggatg gtctggcttg acggcgacga acgtcgcccg
     1261 accggtcgcg gtgaccaccc cgcccgaggc gatctccgcc tcgaccgtcg cccggtcctc
     1321 acccgcctcg acgacccacg cccgcaggtc gaccggccgg tccgacgggg tcgggcggcg
     1381 catccggatc gcgtagtcga gggtgaccgt cgtcggcgga cggtcggcgc cgcgggcgcg
     1441 catcaggtgc caggtcgcgg tccaattggc gtggcagtcg aagagcgtcc cgaggatgcc
     1501 gccgttgacg acgccggcga aggcctcgtg gtcggcgagg ggcgtccagc tggcgacgag
     1561 aacgccagga tcgtcgggat ccgggcggct ctcgacgtgc agcccgatcg ggttggccgg
     1621 cccgcagccg aagcaccgac cgcgcggggc ataccgctgc tgcaggctcg gggttgcgtc
     1681 ggcgctcatc gccgcgagta tcgcagccat ccgcggatcg gtcccgtcgt ggtcggagcg
     1741 cagtcgtggt cggagcgcct tgacgctgcc aactggtcca ccgacgatgg gagcgaggac
     1801 attccgtcag cttggggggc ccgtggatca cgagacgcgc gagacgcccg cgcccgaagg
     1861 cgcgactcgc ggggagaaga ccggtcaccg cggcggccgc ctcggctgcg cggtcgagat
     1921 cgtcgagacc ctcgtcctca cgctcgtcat cttcgtcgtg atccagaatt tcgtggcgca
     1981 gccgttcaag gtggagggga gttcgatgga acggacgttt gtcgagagcg actacgtcct
     2041 cgtggatcgg ctgacgcctc ggtggagccc ctacgctcgc ggacaagtgg tcgtgttcca
     2101 gcctccggag accgtgagcg atcgcaagga accgttcatc aagcgcgtga tcggggttgg
     2161 cggcgac
//
LOCUS       DATAIW010000223         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_161334, whole genome shotgun sequence.
ACCESSION   DATAIW010000223 DATAIW010000000
VERSION     DATAIW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161334"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VHM48_07075"
     CDS             complement(<1..241)
                     /locus_tag="VHM48_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755208.1"
                     /translation="MPLDAPYQVAPLQPGSNGSWSAPSYDVEPGVAVFAGRRLAEDRR
                     ARPELDPVADSDSVPAGPDAAPGRPIRGNHGGRTRT"
     gene            783..1154
                     /locus_tag="VHM48_07080"
     CDS             783..1154
                     /locus_tag="VHM48_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755209.1"
                     /translation="MGRLAMAILGWPPLGLFAAAAIDQSTGCGRYAASCPEMSSPGTW
                     ILQVSILLFLLALPAVAAWSAHGAIAALGVGLPSAIVLSAAGGSNVRETSGPILLAVI
                     AIAYLVGVAYAVVAGRRPAPA"
     gene            1269..1991
                     /locus_tag="VHM48_07085"
     CDS             1269..1991
                     /locus_tag="VHM48_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX2755210.1"
                     /translation="MNRRHDAQDLSSAAQQYLLALRVMAGDGSHVRAAQVARRLGVST
                     QAASEMFRRLVADGLVGQADGRDLVLTTAGRAAADAIFRRHALLEWLLTSVVGLGWAE
                     SDEEAARLQGALSPRVEARLDEMLGHPPTCPHGNPIDAETARQRPRGTPLSEVDSGQR
                     ATIYRITEEAEEDAGLLSYLEARALTPGASVSVLARSESLDSLTLEGPRGRATLGLRP
                     ASLVHVLPGDADPKLFHRVPAR"
     gene            2048..>2211
                     /locus_tag="VHM48_07090"
     CDS             2048..>2211
                     /locus_tag="VHM48_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX2755211.1"
                     /translation="MPDPTIRVRIAPSPTGPLHIGTARTALFNFLFARHVGGTFLFRL
                     EDTDVARSTIA"
BASE COUNT          274 a          869 c          782 g          286 t
ORIGIN      
        1 cggttcgggt ccgcccgcca tggttcccgc ggatcggtcg cccgggggct gcgtccggcc
       61 cggccggaac cgagtcggag tcggcaaccg ggtcgagttc gggccgggcc cggcggtcct
      121 cggccagacg ccgaccggcg aacaccgcga cccccggctc gacgtcgtac gagggcgccg
      181 accaggagcc gttcgaaccc ggctggagcg gcgcgacctg gtacggcgcg tcgagtggca
      241 cgtactggac gatcaacccg aaggaatacg ccgacccgcg caagcacggt ccggaatatc
      301 aagcccgcgc caggcgcgcg cgcgccggga tcgtcgtcga cgcggccggg gttgcgatgc
      361 cgagcgcaac cgatgcggac gggccggcgg cggacgggcc ggcggccgac gtcgacccgg
      421 acaaagccga tcgtcgcgac ggatcgccgg acgctcgccc tctcgaggca gacccggacc
      481 cgcggcccga cggcgaggca ccggccaccc gctcgggcga ccctccgcca cgccccgacg
      541 ccgcggactg ggcgagccgg cgccccggtg gccgccgcca ctggacagac ggccgcccgg
      601 ctgcgggttc gaccgagggt gcgacgggtg acgggctcgg aacccgcccg gacgaacccg
      661 gccgaacgcc cggagatccg ggccgcggcg gcccaccggc gggtggactc cgctcgtgga
      721 tcgaacagcc ggccggcgac gatgccttcc gtgggccgca cctccgccgg cccacgaccc
      781 ccatgggccg cctcgcgatg gcgatcctcg gctggccgcc gctcgggttg ttcgcggccg
      841 ccgcaatcga ccaatcgacc ggctgcggcc gctacgccgc ctcctgtccc gagatgtcgt
      901 cgcccgggac ctggatcctg caggtctcga tcctgttgtt cctgctcgcc ctgccggcgg
      961 tggccgcgtg gtctgcccat ggcgcgatcg cggcgctcgg cgtcgggctg ccatcggcca
     1021 tcgtcctcag cgcggccggc ggctcgaacg tgcgcgaaac gagcggtccg atcctgctgg
     1081 ccgtcatcgc gatcgcctac ctcgtcggcg tggcctacgc cgtcgtcgcc gggcggcgcc
     1141 cggcgccggc ctgacacccg gcggtcgccg tcggatcctc cgtcgacccg ccgcggcggg
     1201 cgcgcccagc acgacgcccg gtcgcgagct ccgttggcgg ccgtccggtg ccgcgtacca
     1261 tgaggcgcgt gaaccgccgc cacgacgccc aggacctgtc gagcgcggcg cagcaatacc
     1321 tcctggcgct ccgcgtcatg gcgggcgacg gctcccacgt ccgggcagcg caggtcgccc
     1381 gacgtctcgg cgtctcgacc caggccgcga gcgagatgtt ccgccggctg gtcgccgatg
     1441 gtctggtcgg ccaggcggac ggtcgcgacc tggtcctcac gacggccggc cgggcagccg
     1501 ccgatgccat cttccgacgc catgccctgc tcgagtggct gctgacgtcg gtggtcgggc
     1561 tcggatgggc cgagtcggac gaggaggcag cccggctcca gggtgcgctc tcgccgcgcg
     1621 tcgaggcccg cctcgacgag atgctcggcc atccgccgac ctgtccccac ggcaacccga
     1681 tcgatgccga gaccgcccgc cagcggccgc gcggcacgcc gctcagcgag gtcgacagcg
     1741 gccagcgggc gacgatctac cggatcaccg aggaagcgga ggaagatgca ggcctcctct
     1801 cctatctcga ggcccgggcg ctgacgccgg gcgcgtcggt cagtgtcctc gcccggtcgg
     1861 aatcgctcga ctcgctcacc ctcgaaggcc cgcgtggccg ggcgacgctc gggctccggc
     1921 cggcttcgct ggtccatgtc ctgcccggcg acgcggatcc caagctcttc catcgggtcc
     1981 ccgctcgctg accgtcgaag gacgaattcc ggcccccggc tgagccgccg ctgctacgct
     2041 ctcgtccatg cctgatccca cgatccgcgt ccggatcgca ccgagcccga ccggccctct
     2101 tcacatcggt acggcccgga cggcgctgtt caacttcctg ttcgcccgcc acgtcggggg
     2161 gaccttcctg ttccggctcg aggacaccga cgtcgcccgc tcgaccatcg c
//
LOCUS       DATAIW010000224         5812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_71992, whole genome shotgun sequence.
ACCESSION   DATAIW010000224 DATAIW010000000
VERSION     DATAIW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71992"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VHM48_07095"
     CDS             <1..1030
                     /locus_tag="VHM48_07095"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX2755212.1"
                     /translation="RPPRPATPRIEAVRVPDRPARSVEVDLRVDLGRGLILPNPILVA
                     SGTFGYGIEYGDVVDVDRLGAICCKGTTLRPRIGNMTPRVTETPGGMLNSIGLQNPGV
                     DAVIEKYAETWAGWKVPVIVNVAGESVADYVEVVRRLEGVPGVAGIELNISCPNIGRG
                     GLQFAIDAGAAGEVTAAVRRATDLPLLVKLSPNVADVRPIARAIADAGADALTAINTL
                     SGIAIAPSRTKPLLGNIYGGLSGPAIKPVALRIVYEVSQIVDIPVVAIGGVTELGDVL
                     DFLAVGAVAVQVGTAIFADPTLPVRLVDELTEVCRARGLSTYADLIGTALPRRTGAPS
                     SKGVEYRP"
     gene            1125..1730
                     /locus_tag="VHM48_07100"
     CDS             1125..1730
                     /locus_tag="VHM48_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755213.1"
                     /translation="MHVVRSFAAAATVILAVTLTACGGGGTPAPANPSAGGGGAAASG
                     TGSQASQATGGSPSANPSQASNGGGGFSGDPCSLVSAADVSGIFGGSPVGFKVNEYGA
                     CTFEINGTLKAGSGGAVGGQCQVKIEDDHTSFDTAKMLFGDAVKKVDGLGADAWSFGG
                     FVHVQIGAQDLIVGGVFVGNYDRDLLASETTALTKLILTRI"
     gene            1779..2462
                     /gene="pyrE"
                     /locus_tag="VHM48_07105"
     CDS             1779..2462
                     /gene="pyrE"
                     /locus_tag="VHM48_07105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX2755214.1"
                     /translation="MTTIKPAIELGARAALANRTEELFRSSGALREGHFQLKSGRHGD
                     AYVEKFAVLSDPAATSELCAFWSTAFANDEDGEDTRVDLVAGPTTGGVILAFETGRQL
                     GVRAIFAEEVRNADGSTRREFRRGFRIEPGERVLLVDDILTTGGSLLAMLPAIEAMGG
                     EIVECVVLVDRSSGGTNTLTSPTTGRVYPLRSLWRLDLPTYEPGTATCPPCANGTPLH
                     TPGSSGTGA"
     gene            2459..2818
                     /locus_tag="VHM48_07110"
     CDS             2459..2818
                     /locus_tag="VHM48_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755215.1"
                     /translation="MTDATPATPGPRRLLALAVAVVAVVAVAVAAATAFGGPARQVET
                     GIVVAVEATSLSSVQGFSIRTVDGRTVDFRVGSIENAATFPPGHLSEHKVSLAPIRVT
                     FVDRDGAHVAVRIEDAP"
     gene            2815..3312
                     /locus_tag="VHM48_07115"
     CDS             2815..3312
                     /locus_tag="VHM48_07115"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2755216.1"
                     /translation="MTDGRTRIPSLGPRGEGWVGAQLILFVAIAGFGVRDLEGHGLGA
                     GMPFIVPAAGLALAAVGLLLAIRAIRDLGRSLSPFPRPPQGATLVDTGAYRFVRHPIY
                     TGLVLAAAGWSLASGSLWAAASSLALLAVLDAKSRREEAWLTETFAGYPAYRARTKRL
                     IPFVY"
     gene            complement(3326..4261)
                     /locus_tag="VHM48_07120"
     CDS             complement(3326..4261)
                     /locus_tag="VHM48_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755217.1"
                     /translation="MTPRPHLAAFVAALAVVIAACGSPSGPTPIPGKSLAPAPSGSAA
                     APVLPVPVSSELRIGENRVVFSLLDPSGQKPAATPDRSLAIGYTGPNGEAIPSASLTF
                     IWAIEGVSGVYIGHATFPSAGQWTAAFTTDAPGSPKVTLPFTFDVKATTQVVWPGDPA
                     PSVTTPTIADVGGDVAKISSDSKPVKRFYETSEDAALASKKPFVLIFATPKFCQTATC
                     GPTLDRLKPVAAAHPELTFINVEPYQLQFADGSLQPVMTGGNLTPVAATTAFKLSTEP
                     YVFVVGADGKVSASFELVFSPDEIEAAIKTVEAAG"
     gene            4599..>5812
                     /locus_tag="VHM48_07125"
     CDS             4599..>5812
                     /locus_tag="VHM48_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX2755218.1"
                     /translation="MTRFQKLAAATVVTAILLVTIGVIVRATGSGLGCPDWPLCHGQL
                     IPAVDDYKAWIEWIHRSVAVIIGFEVIGLAVLAFLDHRDRRVLLGATVVAVLLVGFQA
                     WLGRETVRLGNSGESVTAHLASAMALVGLLVWILARSLYAARLAGSGSQRFTLVAAFG
                     AGSVFALLLFGSQVTATSQWFVFPDWPLMNGGAFPTLTDANSAQVLHRWIAVIVGLIV
                     AVVFIAAWRIRPRPRSIVRLAAIAAALFPIQAVVGGLQILTGLSGWTQTLHLALGAVV
                     WALLVALVSVSYLEGRSRVTEMAGGSGEPAGSRRDGDEAATPRTRTDTVRAYIALTKP
                     RIIELLLVTTVPAMVLATRAVPGIQVGHWLWLTVWTLVGGTLAAGSANAINCYLDRDI
                     DLLMARTRRRPLP"
BASE COUNT          783 a         2092 c         2052 g          885 t
ORIGIN      
        1 tcgaccgcca cgccccgcca cgccgcgcat cgaggctgtc cgggtgccgg atcggccggc
       61 gcgcagcgtc gaggtcgacc tgcgcgtcga tctcggccgt gggctgatcc tgccgaaccc
      121 gatcctcgtc gcgtcgggca ccttcggata cgggatcgaa tacggggacg tcgtcgacgt
      181 cgatcggctc ggcgcgatct gctgcaaggg aacgaccctg aggccgcgga tcggcaacat
      241 gacgccgcgt gtcaccgaga cgcccggcgg catgctcaac tcgatcgggt tgcagaaccc
      301 gggcgtcgac gccgtcatcg agaagtatgc cgagacctgg gccggctgga aggtgccggt
      361 gatcgtcaac gtcgcgggcg agtcggtcgc cgactacgtc gaggtcgtcc gccggctcga
      421 aggcgtgccg ggagtggccg ggatcgagct gaacatctcc tgcccgaata tcgggcgggg
      481 cgggctccag ttcgcgatcg atgccggcgc cgccggtgag gtgacggccg ccgtccgacg
      541 ggcgaccgat ctgccgctgc tcgtcaagct ctcgccgaac gtggccgacg tccggccgat
      601 cgcccgggcc atcgccgacg ccggcgccga cgcgctcacc gcgatcaaca ccctgtccgg
      661 aatcgcgatc gcgccgtcgc gcaccaagcc gttgctcggc aacatctacg gcggcctgtc
      721 cggcccggcg atcaagccgg tggccctccg gatcgtctac gaggtcagcc agatcgtcga
      781 catcccggtg gtcgcgatcg gtggcgtgac tgagcttggc gatgtgctgg acttcctcgc
      841 ggtcggcgcg gttgccgtcc aggtgggcac cgcgatcttc gccgacccga cgctgcccgt
      901 ccggctcgtc gacgagctga cggaggtgtg ccgggcacgc gggctgagca cctacgccga
      961 cctcatcggg acggccctgc cccgccggac gggcgccccg tcgtcgaagg gcgtggagta
     1021 tcgtccatga cggcgcggct gaacggcgtc gacggaccct gagcccgtcg gtttccgaag
     1081 cgcgccgatc ccgaccgtca tcgtccgcaa ccggggagtc ctcgatgcac gttgttcgat
     1141 cgttcgcggc ggccgcgacc gtcatcctcg ccgtcaccct cacggcctgc ggcggcggcg
     1201 gcacaccggc ccctgcgaat ccctcggccg gcggcggtgg cgcggccgca tcgggcacgg
     1261 gcagtcaggc ttcccaggcg accggtgggt ccccctcggc gaacccgtcg caggcgtcga
     1321 acggcggcgg ggggttctcc ggcgacccgt gctcactcgt ctccgctgcc gacgtcagcg
     1381 ggatcttcgg cggctcaccg gtcggtttca aggtcaacga gtacggcgcc tgcacgttcg
     1441 agatcaacgg cacgctcaaa gccggctccg gcggtgccgt cggcggtcag tgccaggtga
     1501 agatcgaaga cgaccacacg tcgttcgata cggccaagat gctgtttggt gatgccgtca
     1561 agaaggtcga cggcctcggc gccgacgcgt ggtcctttgg cggattcgtc cacgtccaga
     1621 tcggcgctca ggacctgatc gtgggcggcg tcttcgtcgg caactacgac cgcgaccttc
     1681 tggcgtccga aacgaccgca ctcacgaagc tgatcctgac ccgcatctga ccccccgtcg
     1741 gtgcggtcgt cgcggcccga aggctagact tcgaggtcat gacgaccatc aagcctgcca
     1801 tcgaactcgg tgcgcgcgcc gccctcgcca accggaccga ggagctgttc cgctcgtccg
     1861 gcgccctgcg ggaaggccac ttccagctga agagcggccg gcacggcgat gcctatgtcg
     1921 agaagttcgc ggtgctgtcc gatcccgccg cgacgagtga gctgtgcgct ttctggtcga
     1981 ccgcgttcgc caatgacgag gacggcgagg acacaagggt cgacctcgtc gccggcccca
     2041 cgaccggtgg cgtgatcctg gcgttcgaga cgggccgaca gctcggggtc cgggcgatct
     2101 tcgcggagga ggtccgcaac gccgacgggt cgacgcgacg cgaattccgg cgcggcttcc
     2161 ggatcgagcc cggtgagcgc gtcctgctcg tcgacgacat cctcacgacg ggcggctcac
     2221 tgctggcgat gctgccggcg atcgaggcga tgggcggcga gatcgtcgaa tgcgtcgtcc
     2281 tggtcgaccg cagcagcggc gggacgaata cgctgacctc gccgacgacg ggacgggtct
     2341 acccgctgcg ctcactctgg cgcctcgacc tgccgaccta cgagcccggc accgcgacct
     2401 gcccgccctg cgccaacggc acgccgctcc acactccggg gagcagcggg accggcgcgt
     2461 gaccgacgca actcccgcga caccggggcc gcgccggctc ctcgccctgg cggtcgcggt
     2521 cgtcgcggtc gtcgcggtgg ccgtggcggc ggcgaccgcg ttcggcgggc cagctcgaca
     2581 ggtcgagacc gggatcgtgg tcgccgtcga ggcgacgagc ctcagctccg tccaggggtt
     2641 ctcgatccgg acggtggatg gccggacggt cgacttccgg gtcggctcga tcgagaacgc
     2701 ggcaaccttc ccgccgggcc acctgtccga gcacaaggtc agcctcgccc cgatccgggt
     2761 gacgttcgtc gatcgcgacg gcgcccacgt ggcggtccgg atcgaggacg cgccgtgacc
     2821 gacggccgca cgcggatccc gtccctcggc ccgcgcggcg agggctgggt cggcgcccag
     2881 ttgatcctgt tcgtcgccat cgccgggttc ggcgttcggg acctcgaggg acacgggctc
     2941 ggggccggta tgccgttcat cgtccccgct gcgggcctcg cgctcgcggc ggtggggctg
     3001 ctgctcgcca tccgcgctat ccgcgacctc ggccggagcc tcagcccgtt cccgcggccg
     3061 cctcagggcg ccacgctcgt cgacaccggc gcctaccgat tcgtgcgaca cccgatctac
     3121 accgggctgg tgctggcggc cgccggctgg agcctcgcct cgggctcgct ctgggccgca
     3181 gcgtcgtccc tcgcgctgtt ggccgtcctc gacgccaagt cgcggcggga ggaggcctgg
     3241 ctgaccgaga ccttcgccgg ctaccccgcc taccgggccc ggacgaagcg gctgattccg
     3301 ttcgtgtact gatcgcccgg atcggtcagc cggccgcctc gaccgtcttg atcgccgctt
     3361 cgatctcgtc gggcgagaac acgagctcga aggacgcgct gaccttcccg tcggccccga
     3421 cgacgaacac gtacggctcg gtcgagagct tgaacgcggt cgtcgccgcg accggcgtca
     3481 ggttgccacc cgtcatcacc ggctgaagcg acccgtcggc gaactggagc tggtagggct
     3541 cgacgttgat gaaggtcagc tccgggtgcg ccgcggcgac gggcttgagg cggtcgaggg
     3601 tcgggccgca ggtcgcggtc tggcagaact tcggtgtcgc gaagatgagg acgaacggct
     3661 tcttcgaggc gagcgcagcg tcctccgagg tctcgtagaa ccgcttcacc ggcttggagt
     3721 cgctcgagat cttcgccacg tcaccgccga cgtcggcgat ggtcggcgtc gtgaccgagg
     3781 gcgccggatc gcccggccag acgacctggg tcgtcgcctt cacgtcgaat gtgaagggca
     3841 gggtcacctt gggcgagccg ggtgcgtcgg tcgtgaacgc cgccgtccac tggccggccg
     3901 acgggaatgt cgcgtgcccg atgtacacgc cgctgacccc ctcgatcgcc cagatgaacg
     3961 tcaacgacgc ggacgggatc gcctcgccgt tggggccggt gtagccgatc gccaggctgc
     4021 ggtcgggtgt cgccgcgggc ttctggccgg acgggtcgag cagggagaag acgacccggt
     4081 tctcgccgat ccgcaactcg ctcgagaccg gcaccggcag gacgggcgcg gcggccgagc
     4141 ccgagggcgc cggcgcgagc gacttgcccg ggatcggcgt gggtcccgag ggggagccgc
     4201 acgccgcgat cacgacggcc agggcggcga cgaacgccgc gagatgaggt cggggggtca
     4261 tggtccgtag cctagaacgg gacgctcacc tccgctttgc acgagcgccg ccaacacccg
     4321 agcgtcgcca agagaccgcg cccggcgcac ttcgaatagc cgaatacgac ggggacaggc
     4381 ggcgcccggt gcgcgtccgt cctgggctca tcccgacctg cgagattcga atcattcggc
     4441 tatccgcagc gcagcggcgg gcgagttccc agggcctgcg gacggccagc cgcgcgagac
     4501 tggcccgcac aagcgctcac gctagaatgg cgggcgtgcg gccgcggtcc ggcccggccg
     4561 cgcccggcat tccgaccccg cgttccccga ggaggctcgt gacccgcttc cagaagctcg
     4621 ccgcggcaac cgtcgtgaca gcgatcctgc tcgtcacgat cggggtcatc gtccgggcga
     4681 ccggatcggg actcggctgc ccggattggc cgctgtgcca tggccagctc atccccgccg
     4741 tcgacgacta caaggcgtgg atcgaatgga tccaccggag cgtcgcggtg atcatcggct
     4801 tcgaggtcat cgggctggcc gtcctcgcct tcctcgacca ccgcgaccgg cgcgtcctcc
     4861 tcggggcgac cgtcgtcgcg gtcctcctcg tcggcttcca ggcctggctc gggagggaga
     4921 ctgtccggct cggcaactcc ggcgagtcgg tcaccgccca tctcgcctcg gcgatggccc
     4981 tcgtcgggct gctcgtctgg atcctggccc gctcgctgta cgccgcccgg ctcgccggct
     5041 cgggcagcca gcggttcacg cttgtcgccg cgttcggcgc cggctcggtg ttcgccctgc
     5101 tcctgttcgg gtcgcaggtc acggcgacct cccagtggtt cgtcttcccg gactggccgc
     5161 tgatgaacgg cggggcgttc ccgacgctca ccgacgccaa ctcggcccag gtcctccacc
     5221 gctggatcgc cgtcatcgtc ggactcatcg tcgctgtcgt cttcatcgcg gcctggcgca
     5281 tccggccccg cccccggtcc atcgtccggc tggccgcgat cgcggccgcg ctgttcccga
     5341 tccaggcggt cgtcggcggc ctccagatcc tgacgggcct gtcgggctgg acgcagacgc
     5401 tgcacctcgc gctgggggcg gtcgtctggg cgctgctcgt ggcgctggtc agcgtgtcgt
     5461 acctcgaagg ccgctcccgg gtcaccgaga tggcaggcgg aagcggcgaa ccggcaggca
     5521 gccgccggga cggcgacgag gcggcgacgc cccggacgcg gacggacacc gtccgggcct
     5581 acatcgcgct gaccaagccg cggatcatcg agctcctgct cgtgacgacc gtcccggcga
     5641 tggtcctcgc gacccgcgcg gtaccgggca tccaggtcgg gcactggctg tggctgaccg
     5701 tctggacgct cgtcggaggg acgctcgcgg cgggcagcgc gaatgcgatc aactgctacc
     5761 tcgaccgcga catcgacctg ctcatggccc ggacgcggcg tcgccccctg cc
//
LOCUS       DATAIW010000225         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_243622, whole genome shotgun sequence.
ACCESSION   DATAIW010000225 DATAIW010000000
VERSION     DATAIW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243622"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..613)
                     /locus_tag="VHM48_07130"
     CDS             complement(149..613)
                     /locus_tag="VHM48_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2755219.1"
                     /translation="MARPTAWLVLAGAVVAVLIAGIVAVGMFNGSRTGAEGTTVQARG
                     GHWTNITADRLATMLGQKDFTLLNVKTPYSGEIARTDLYIPYDQLTARTAELPADKTA
                     MVVVYCRTGHESAIAAQTLLDLGYSNIDNLDGGMTAWTAGGRQLVTLDRTGG"
     gene            917..1291
                     /locus_tag="VHM48_07135"
     CDS             917..1291
                     /locus_tag="VHM48_07135"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2755220.1"
                     /translation="MPSATNDIDELQASLLRSLASAHRIRILHLLGERPCEVNEVARE
                     LEMSQATASQHLGAMRGVGLVEAVRDGRNVSYRVSDPQILVACGLMRDVLVRRLSRLG
                     DLAAAVHESTLVLVSSSQEGHP"
     gene            1288..1695
                     /locus_tag="VHM48_07140"
     CDS             1288..1695
                     /locus_tag="VHM48_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX2755221.1"
                     /translation="MSDNLSIVLFSGTDDKLHAAATITAGAAAMGTPVSVLLMYWALD
                     AFRADRIANDHGLAYDASRPRWNEPVEHVGDSPWQDTFRQAKDLGDVTIRACAGSLAT
                     LGLDAAALDPLVDDAGGIAAFLMAADGGQILFI"
     gene            1726..2091
                     /locus_tag="VHM48_07145"
     CDS             1726..2091
                     /locus_tag="VHM48_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2755222.1"
                     /translation="MADQEKLVIVATHGPDDPERATIPFVMACAALASDVAVVMGFQA
                     DGVRLVQQGEAEKVQAPEFPPLAKLLDDFRELGGTLLVCSPCVKSRGISESLVPGAEI
                     VAAARFVAEVTSATNALVY"
     gene            2154..2429
                     /locus_tag="VHM48_07150"
     CDS             2154..2429
                     /locus_tag="VHM48_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2755223.1"
                     /translation="MTTMTAPLSDTDLIALETAKVVDARGSACPGPLLEAKKAMNTVQ
                     IGQTIEIWSSDPATKTDIGAWSAKVGHTYLGTLPGEGYERTFVVRQK"
     gene            2477..2923
                     /locus_tag="VHM48_07155"
     CDS             2477..2923
                     /locus_tag="VHM48_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX2755224.1"
                     /translation="MTTLAPERPQEAERTFVPRILVFSTNNISDPGIDLAGSAHMTYS
                     PGVMVISLPCTSGIRPSWIRHAIEHGFDGVFIASDGDECAYLADCSTRASRIVAEAQE
                     LLVKDGHQAARLKMAAICSVCAEPFTRHVEQFGQALAALGPSVVTS"
     gene            2920..>5559
                     /locus_tag="VHM48_07160"
     CDS             2920..>5559
                     /locus_tag="VHM48_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2755225.1"
                     /translation="MSDYDVLVVGAGIGGMESALKLGDMGYRVMLVEKEASVGGKMIL
                     LSKVFPTLDCASCISTPKMAASIHHPNVTTLTYCEVEGIEPVGEGRFHATIRRKARFV
                     DEAACTGCQKCETACTVSVPDQFNADLVARHAAYIAFPQAVPKKAVIERAGSSPCSFT
                     CPAGIQAHGYVSLIRSGEYEKAFQLVLDATPLVGTLGRACYAPCEVDCTRGSLEGPLP
                     IRRLKRFIADEHYDRFDGPGVAIPEPNGHRVAIVGSGPAGLTAAWQLARKGYGVKIFE
                     AAPKAGGFLRLAIPAYRLPSEVVERDIANVTAIGVEIAVDTPVEDLEGLRGDGYDAVL
                     VATGTPLSTRMGVPGEQLEGVLSGVAFLRSVKLGEGIDLTDRRVVVIGGGNVAMDTAR
                     TARRLGARDVSVVYRRTRAEMPAHHVEAADTEKEGVGFVYQTAPLAVLDDDHGSTRAL
                     RCSRMTPGTPDASGRSRPEPVPGSEFEIDCDVIITAIGMAPDSAAFASQVGATRTGSI
                     VVDERTLQTDVPWLFAAGDVVSGASDITRATGQGQRAAFMIDRWIQDRTLEGFDLRLP
                     VVDKDEVLARQKAYRLALPAAPSEVIEAAPTDFREVELPMTEAEARAGAGLCLDCGVC
                     SECGECVDACPVENCIDLRARDQISEIEVGAVVVATGFELFAADLKPQYGFGTFKNVI
                     TGMQMDRLLAPTRPFNTILRPGDGKVPERIAYVMCTGSRDETVGNPLCSRFCCMYSIK
                     QNQLIMGALPIADVTVHYMDIRAAGKRYDEFYEQAKAMGANYVKGRVAKITEKDDGDL
                     VLRYEDIEHGGQLVEAEYDLVVLAVGVQPNSDAQALFPEGGLSLDEFQYVAETDEDAN
                     PGQTSIPGVYVAGAASGAKDIADS"
BASE COUNT          872 a         1981 c         1866 g          840 t
ORIGIN      
        1 acgacgcgcg ccgcgcggcc ccagcgcgga tcgacccgtc catcgggcgt gatgggaatg
       61 cagatgagca catcgtctcc ttgtcaccgt gttgtctggg cgtcggatgg atcggcaccc
      121 gacccggagc tcgccgcccg cgcccgcctc agccgccagt ccgatcgagg gtgaccagct
      181 gtctgccgcc ggccgtccag gcggtcattc ctccatcaag gttgtcgatg ttggagtaac
      241 cgaggtcgag cagcgtctgg gcagcgatcg cactctcgtg tccggtccgg cagtagacga
      301 cgaccatcgc ggtcttgtcg gccggcagct cggccgtccg tgcggtgagc tggtcgtacg
      361 ggatgtagag gtcggtccgg gcgatctcgc cgctgtatgg cgtcttcacg ttgagcaggg
      421 tgaagtcctt ctggccgagc atcgttgcca ggcggtcggc cgtgatgttc gtccagtggc
      481 cgccgcgcgc ctgcacggtc gttccctcag cgcccgtcct cgatccgttg aacattccga
      541 cggcgacgat gccggcgatc aggacggcca cgaccgcgcc cgcgaggacc agccaggccg
      601 tcggacgcgc caagcggcgg gatcgcccgc ccggcgccga tcgagagggc gacgggaccg
      661 acgcacggcc gtgcctggcg gcccggtgtt cacgttctgc tgaccgcgcc gatcgcggga
      721 tgcctcgctt gctcacgctc gcctccagtg tcccgagtgg agcgttgccg atcggcgcgg
      781 tcggccggac cgtccaatcg gcccacaatc ccgtgactaa tggcccacag cctatattcg
      841 tcttagacat atattggcgt tgaaccactg cggcgcacgg aaggcgagac agcccggccg
      901 ccgcgaggag cagcacatgc caagcgcgac gaatgacatc gatgagctcc aggcgtcgtt
      961 gctccggtcg ctcgcgagcg cccaccgcat ccgcatcctc cacctgctcg gtgagcggcc
     1021 ctgcgaggtc aacgaggtcg cccgcgagct cgagatgagc caggcgaccg cctcccagca
     1081 tctcggcgcg atgcgcggcg tcggcctcgt cgaggcggtc cgcgacggac gcaacgtgag
     1141 ctaccgggtc agcgacccgc agatcctcgt ggcctgcggc ctcatgcgcg acgtcctcgt
     1201 ccgccgtctg tcgcggctgg gcgacctggc cgcagcggtc cacgagtcga ccctcgtcct
     1261 cgtctccagc agtcaggaag gtcatccgtg agcgacaacc tgtccatcgt cctgttctcg
     1321 ggcaccgacg acaagctcca cgccgctgcc acgatcacgg cgggcgcggc cgcgatgggg
     1381 acgcccgtca gcgtcctcct catgtactgg gcgctcgatg ccttccgcgc cgatcggatc
     1441 gcgaacgatc acggtctcgc ctacgacgcc tcccgtcccc gctggaacga gcccgtcgag
     1501 cacgtcggcg acagcccgtg gcaagacacg ttccgccagg cgaaggacct cggcgatgtg
     1561 accatccggg cctgcgccgg gtcgctcgcg accctcggcc tcgatgccgc cgcgctcgac
     1621 ccgctcgtcg acgacgcggg tgggatcgct gcgttcctca tggccgccga tggtggccag
     1681 atcctcttca tctgaaccag cggacatagg gaaaggagcc tcgtcatggc cgaccaggag
     1741 aagctcgtca tcgtcgccac tcacggcccg gatgacccgg agcgggcgac gatcccgttc
     1801 gtgatggcct gcgccgcgct cgcttcggac gtcgccgtcg tcatgggctt ccaggccgat
     1861 ggtgtccgtc tcgtccagca gggtgaggcc gagaaggtcc aggcaccgga gttcccaccg
     1921 ctcgccaagc ttctcgacga cttccgcgag ctcggcggca cgctcctcgt ctgctcgcca
     1981 tgcgtcaaga gccgcgggat cagcgagtcg ctcgtgccgg gcgcggagat cgtcgccgcg
     2041 gcccgcttcg ttgcggaggt gacgtcggcc accaacgcgc tcgtctactg agccagcacc
     2101 gttccggccg cgcgatcggc gcggccgacc aggcaccgaa ggagaccatc gagatgacca
     2161 ccatgaccgc accgctgagt gacaccgacc tcatcgcgct cgagaccgcg aaggtcgtcg
     2221 acgcccgcgg cagcgcctgc ccgggcccgc tcctcgaggc caagaaggcg atgaacaccg
     2281 tccagatcgg tcagacgatc gagatctggt cgagcgatcc ggcgaccaag accgacatcg
     2341 gtgcctggtc ggcaaaggtc ggccacacct acctcggaac cctgccgggc gagggctacg
     2401 agcggacctt cgtcgtccgc cagaagtagc ggcccgccag ccggctgatg cccgtgccgc
     2461 caaggagtca tgtgcgatga ccacgcttgc gccggaaagg ccgcaggagg cggaacgaac
     2521 cttcgttccg cgcatcctgg tcttctcgac caacaacatc tccgacccgg gcatcgacct
     2581 ggccggaagc gcccacatga cctattcgcc gggtgtcatg gtcatcagcc tgccctgcac
     2641 cagcgggatc cggccgagct ggatccgcca cgccatcgag cacggcttcg acggcgtgtt
     2701 cattgcctcc gacggcgacg agtgcgcgta cctggccgat tgctccacgc gagcctcgcg
     2761 gatcgtcgcc gaggctcagg agctcctggt caaggacggc caccaggcgg cgcggctgaa
     2821 gatggccgcg atctgctcgg tctgcgcgga gccattcacg agacacgtcg agcaattcgg
     2881 ccaggcgctt gccgcgctgg gcccgtcggt ggtgacgtca tgagcgacta cgacgttctc
     2941 gtcgtcggcg ccgggatcgg cggcatggag tccgccctca agctcggcga catgggctac
     3001 cgggtcatgc tcgtcgagaa ggaagccagc gtcggcggga agatgatcct gctcagcaag
     3061 gtcttcccga ccctcgactg cgcgagctgc atctcgaccc cgaagatggc cgcgtcgatc
     3121 caccacccga acgtcacgac cctgacctac tgcgaggtcg agggcatcga gcccgtcggc
     3181 gagggccggt tccacgccac gatcaggcgc aaggcgcggt tcgtcgacga ggcggcctgc
     3241 accggctgcc agaagtgcga gacggcctgc acggtctcgg tccctgacca gttcaacgcc
     3301 gacctggtgg cccgccatgc ggcgtacatc gccttccccc aggccgtgcc caagaaggcg
     3361 gtcatcgagc gagcgggttc gtcaccgtgc tcgttcacct gccctgccgg catccaggcc
     3421 cacggctacg tctcgctcat ccggagcggc gaatacgaga aggcgttcca gctcgtgctc
     3481 gacgccacgc ccctcgtcgg cacgctcggt cgcgcgtgct acgcgccgtg cgaggtcgac
     3541 tgcacccgcg gctcactcga aggtcccctc ccgatccgac ggctcaagcg cttcatcgcg
     3601 gacgagcact acgaccggtt cgacggcccc ggggtggcga tccccgagcc gaacggccac
     3661 cgtgtcgcga tcgtcggctc cgggccggct ggcctgacgg cggcctggca gctcgcccgc
     3721 aagggctacg gcgtgaagat cttcgaggcg gcaccgaagg ccggcggctt cctccgcctc
     3781 gccatccccg cctatcgcct gccctcggag gtcgtggagc gggacatcgc gaatgtcacg
     3841 gccatcggcg tcgagatcgc cgtcgacacg cccgtcgagg acctcgaagg gctccgtggg
     3901 gatggctacg acgcggtcct cgtggcgacc gggacgccgc tctcgacccg gatgggcgtg
     3961 cccggcgagc agctcgaggg cgtcctgagc ggcgtcgcat tcctgcgctc ggtgaagctc
     4021 ggcgagggga tcgacctgac cgaccggcgc gtcgtcgtca tcggcggcgg caacgtcgcg
     4081 atggacactg cgcggaccgc ccgccggctc ggcgcccgcg atgtcagcgt cgtctatcgc
     4141 cgcacccggg cagagatgcc ggcccatcac gtcgaggccg ccgacaccga gaaggagggc
     4201 gtcgggttcg tctaccagac ggcgccgctc gcggttctcg acgacgacca cgggtcgacg
     4261 cgggccctcc gctgcagccg gatgaccccg ggaaccccgg atgcatctgg tcggagccgg
     4321 cccgagccgg tcccgggcag cgagttcgag atcgactgcg atgtgatcat caccgcgatc
     4381 ggcatggccc ccgacagcgc ggcgttcgcg agccaggtcg gggcgacgag gaccgggtcg
     4441 atcgtcgtgg acgagcggac gctccagacc gacgtgccat ggctgttcgc cgccggcgac
     4501 gtcgtatcgg gtgcatcgga catcacccgg gcgaccggcc agggccagcg ggccgcgttc
     4561 atgatcgatc gctggatcca ggatcggacg ctcgagggct tcgacctccg cctgccggtc
     4621 gtcgacaagg acgaggttct ggcccgccag aaggcgtacc gcctggcgct gcctgcggct
     4681 cccagcgagg tcatcgaggc cgcaccgacc gatttccgcg aggtggagct gccgatgacc
     4741 gaggccgagg cacgcgcggg cgccgggctg tgtctcgact gcggcgtctg ctcggagtgc
     4801 ggcgagtgcg tcgacgcctg cccggtcgag aactgcatcg acctccgcgc ccgcgaccag
     4861 atcagcgaga tcgaggtcgg ggcggtcgtc gtggcgaccg gcttcgagct cttcgccgcc
     4921 gacctcaagc cgcagtacgg cttcggcacg ttcaagaacg tcatcaccgg catgcagatg
     4981 gaccgcctgc tcgctccgac ccggccattc aacacgatcc tccggccggg tgacggcaag
     5041 gtccccgagc ggatcgccta tgtcatgtgc accggatccc gcgacgagac cgtcggcaac
     5101 ccgctctgct cgcggttctg ctgcatgtac tcgatcaagc agaaccagct gatcatgggc
     5161 gccctgccga tcgccgacgt gacggtccac tacatggaca tccgggccgc cggcaagcgc
     5221 tacgacgagt tctacgagca ggccaaggcg atgggcgcca actacgtcaa gggacgggtg
     5281 gcgaagatca ccgagaagga cgacggcgac ctggtcctgc gctacgagga catcgagcac
     5341 ggggggcagc tggtcgaggc cgagtacgac ctcgtcgtgc tggccgtcgg ggtccagccc
     5401 aacagcgatg cccaggccct cttcccggag ggcggcctgt cgctcgacga gttccagtac
     5461 gtcgccgaga ccgacgagga cgccaacccg ggtcagacca gcatcccggg cgtgtacgtc
     5521 gccggcgccg catccggcgc caaggacatc gccgattcg
//
LOCUS       DATAIW010000226         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_101484, whole genome shotgun sequence.
ACCESSION   DATAIW010000226 DATAIW010000000
VERSION     DATAIW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101484"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VHM48_07165"
     CDS             complement(<1..1008)
                     /locus_tag="VHM48_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2755226.1"
                     /translation="MTTTNPDPAMTPEEILAARRQRLGPSLSLSYRTPLHIVRGEGAF
                     LYDAGGRAYLDCVNNVAHVGHAHPRVVEAGVAQMRLLNTNTRYLHENVVRYAERLTAL
                     LPGHLAVCFFTNSGSEANELALRLARAATGRPDVATVDVAYHGNTQRLIEISPYKFDG
                     PGGAGRRDDVQVVPLPDPYRGRHRGPGPEVGAVYLAEARAVLDSSAVAGHPAGTLIAE
                     AIPSVAGQVVPPPGWLAGLFDAARAVGAVPIADEVQVGFGRVGSAVWAFDAQGARPDI
                     VTMGKPIGNGHPLGAVVTTRAIADAFANGMEYFNTFGGNPVSAAIGLAVLDVIADEGL
                     QE"
     gene            complement(1005..2003)
                     /locus_tag="VHM48_07170"
     CDS             complement(1005..2003)
                     /locus_tag="VHM48_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2755227.1"
                     /translation="MNELVGAVLTRPSIDTAGAASVLLRQWGLVGDLRSLPSERDRNF
                     AVVVDGVERFVLKLSNANEDPAFLDFQHRVMDRLAGAGVPCQRAIRTTAGREIVDIGS
                     DGSPILARLLGWLPGRPLAAIPSAHRSSALLTDLGRTMGRTAAALDGFDHPAAHRRFH
                     WSAEQSLEVIAAHAPAITDPDRVALLDRWRRRLEPLAAILPALRHGVIHNDANDHNVL
                     VADDGASISGLLDLGDAVWSVVVNELAVACAYAALGDADPVATIETVRSGFEEALPLA
                     DDERDLLVELVALRLATSVALSAHQSSLAPDDPYLTVSEAPAWDLLERLIAVEPVR"
     gene            complement(2059..>4650)
                     /gene="dnaE"
                     /locus_tag="VHM48_07175"
     CDS             complement(2059..>4650)
                     /gene="dnaE"
                     /locus_tag="VHM48_07175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2755228.1"
                     /translation="VLGHSYGEIDRIAKAVPNQLGIKLDEAIVTSSQLREMYEGDPAV
                     HRVVDFAKQLEGVARNASTHAAGVVISREPLTDLMPLQRATNSDSLMTQYEMHGVEAL
                     GLLKFDFLGLSNLTILRQAVDLILQERGGERIDIDHIPLDDTRTFELLSSGETTGVFQ
                     LESAGMRRYIRELRPTSVYDLAAMVALFRPGPMDHIPAYIRRKHGLEPVSYLHPLLQP
                     FMEKTYGIFVYQEDIMAAAIALGGFTGPEADTLGYAIRKKKSDVLRAQKGKFVSQAAE
                     RGVEPGVIDAVFKAFEPFERYGFNKAHATCYGLIAYQTAYLKANYTVEYMTAVLTAFR
                     SNEEKVAAAIAECRRMGIEIKLPDVHHSDVEFTVEGPAIRFGLLAVKNVGQSAIESIV
                     AARKEGGPFKSLTDLCSRIDLRLVNRKVLEALAKVGALNALGHPAQVLLGLDDAINAG
                     QAATRDRLSGQTSLFDLGADDATALERPLPFATEVPVRERLRWEKELLGLYLSEHPMG
                     EVAEQVGQYVTAFSSDLKDESLDGQRVVIGGIVTGFRRVITKANATMGVATLEDLQGT
                     IEVVVFPRMYELTAATWTEGTILLVAGRIDHRGDDVSLLADLAVDWDGAITRGPEAFA
                     REVSAGDRGRGGRRGGGANGNGNGWSNGGGPGQRPATDRPAPVPVMATSPMPEPGWGP
                     VGVAVGPGAPPPAAARPDEIRPGVPRVSPLRAEAQIGGDLPRIVPAEPIPTYDEPAGF
                     VGAAASGGGFDDADVEPALPDEARARAAQASQAPTSQIASQPDQVLHVRFGGATSDRL
                     VLAMETFKNLLRERPGGTRVVLHVPAPTGGGSLPMELSRRVAYDAELLSEVRRRLGEG
                     LVDLTLG"
BASE COUNT          670 a         1613 c         1710 g          657 t
ORIGIN      
        1 ctcctggagc ccctcgtcgg cgatgacgtc gagcacggcg agcccgatcg cggccgacac
       61 gggattgccg ccgaacgtgt tgaagtactc catcccgttg gcgaacgcgt cggcgatcgc
      121 gcgggtcgtc accacggcgc ccaggggatg gccattgccg atcggcttgc ccatcgtgac
      181 gatgtccgga cgggcaccct gcgcgtcgaa cgcccatacg gccgaaccga cccgcccgaa
      241 cccgacctgg acctcgtcgg cgatcgggac cgcgccgaca gcgcgggcgg catcgaacag
      301 gccggcgagc cagcccggcg gcgggacgac ctgaccggcg acgctcggga tcgcttcggc
      361 gatcagggta cccgccgggt ggcccgccac ggccgacgaa tccagcaccg cccgggcctc
      421 ggccagatag accgcgccga cctccgggcc ggggccgcga tggcggccgc ggtacggatc
      481 cggaagcggc acgacctgga cgtcgtcacg ccggccggca ccgcccggac cgtcgaactt
      541 gtacgggctg atctcgatga gccgttgggt gttgccgtgg taggcgacgt cgacggtcgc
      601 gacatccggc cgcccggtcg cggctcgggc gagccggagc gccagctcgt tcgcctcgct
      661 gcccgaattc gtgaagaagc agaccgcgag gtggcccggc aggagcgccg tcagacgctc
      721 ggcgtagcgg acgacgttct cgtggaggta gcgcgtgttc gtgttcagga ggcgcatctg
      781 ggcgacgccc gcctcgacga cccgggggtg ggcgtggccg acgtgggcga cgttgttgac
      841 gcagtcgaga tacgcccggc caccggcgtc atacaggaag gcgccctcgc cgcggacgat
      901 atggagcggt gtccgatagc tgagcgaaag cgatggtccg aggcgctgcc ggcgggcggc
      961 caggatctcc tcgggcgtca tcgcgggatc ggggttcgtc gtcgtcaccg gacgggctcg
     1021 actgcgatca gccgctcgag gaggtcccac gccggcgcct ccgagacggt caggtacgga
     1081 tcgtcggggg ccaggctcga ctggtgggcg ctgagcgcga cgctggtcgc gaggcggaga
     1141 gccacgagct cgacgaggag atctcgctcg tcgtccgcga gcggcagcgc ctcctcgaag
     1201 cccgaccgga cggtctcgat cgtcgccaca gggtctgcat cgccaagcgc ggcgtaggcg
     1261 caggcgacgg ccagctcatt gaccacgacc gaccagacgg cgtcgccaag gtcgagcagg
     1321 ccgctgatcg atgcgccgtc gtcggcgacg agcacgttgt ggtcgttcgc gtcgttgtgg
     1381 atgaccccgt ggcggagcgc cggcaggatt gccgccagcg gctcgagacg ccgccgccag
     1441 cgatccagca gggcgacccg gtcggggtcg gtgatcgcgg gggcatgggc cgcgatcacc
     1501 tcgaggctct gctcggcgga ccagtggaac cggcgatgag ccgccggatg atcgaagccg
     1561 tcgagcgcgg ccgcggtccg gcccatcgtg cgtccgaggt ccgtcagcag agccgaggag
     1621 cggtgcgcgg acgggatcgc ggcgagcggc cgacccggca gccagcccag gagccgggcg
     1681 aggatcggcg atccgtcgga cccgatgtcg acgatctcgc gcccggcggt cgtccggatc
     1741 gcccgctggc acgggacacc cgcgccggcc agccgatcca tcacccggtg ctggaagtcg
     1801 aggaaggctg ggtcctcgtt cgcgttcgac agcttcagga cgaaccgctc gacgccgtcg
     1861 acgacgacgg cgaagttccg gtcgcgctcg ctgggcaggg agcgcaggtc gccgaccagc
     1921 ccccactggc ggagcaggac cgaggccgcg ccggcggtgt cgatcgatgg ccgggtgagg
     1981 accgcgccga cgagctcgtt cacccggaca gcgtaccggg ctcgtggtgc gcggcgtcgg
     2041 gtggcgggcg accgagcctc aacccagggt caggtcgacc agtccctcgc cgaggcggcg
     2101 gcggacctcg gagagcagct cggcgtcgta ggcgacgcgc cggctgagct ccatcggcag
     2161 cgagccgcca ccggtcggcg ccgggacatg gaggacgacg cgggttccgc cgggtcgctc
     2221 gcgcagcagg ttcttgaagg tctccatcgc gaggaccagc cggtcggagg tcgcgcctcc
     2281 gaacctgaca tggagcacct ggtcaggctg gctcgcgatc tgggaggtcg gcgcctgcga
     2341 cgcctgggcg gcccgggcgc gcgcctcgtc ggggagggcc ggctcgacgt ccgcatcgtc
     2401 gaacccgccg cccgacgcgg ccgcgccgac gaagcctgcc ggctcgtcgt aggtcgggat
     2461 cggctcggcc gggacgatcc gcggcagatc gccgccgatc tgcgcctcgg ctcggagcgg
     2521 ggagacccgc ggcacgccgg gccggatctc gtctggtcgt gctgcggccg gaggcggggc
     2581 gccggggccg accgcaacgc cgaccgggcc ccagcccggc tccggcatgg gcgaggtagc
     2641 catgacgggg acgggcgccg ggcggtcggt cgccgggcgc tggccgggac cgccgccatt
     2701 cgaccagccg ttgccgttgc cgttggctcc cccgccccgc cgcccgccgc gcccgcgatc
     2761 gccggccgag acctcgcgcg cgaacgcctc gggtccgcgg gtgatcgcac cgtcccagtc
     2821 gaccgcgaga tcggccagca gcgagacgtc gtcgccgcgg tggtcgatcc ggccggcgac
     2881 gagcaggatc gtgccctccg tccaggtggc cgccgtcagc tcgtacatcc tcgggaacac
     2941 gacgacctcg atcgtcccct ggaggtcctc gagcgtcgcc acgcccatcg tcgcgttcgc
     3001 cttcgtgatc acccgccgga agccggtcac gatcccgccg atcacgaccc gctggccgtc
     3061 gagcgactcg tccttgaggt cgctgctgaa ggcggtcacg tactggccga cctgctcggc
     3121 gacctcgccc atcggatgct ccgacaggta caacccgagc agctccttct cccagcgcag
     3181 ccgctcccgg accggcacct cggtggcgaa gggcaacggc cgctccaggg cggtggcatc
     3241 gtcggccccg aggtcgaaca gcgaggtctg gccgctcagc cggtcgcggg tcgccgcctg
     3301 accggcgttg atcgcatcgt cgaggccgag gagcacctgg gcgggatggc cgagggcgtt
     3361 cagggcgccg accttggcga gcgcctcgag gaccttgcgg ttgacgaggc ggaggtcgat
     3421 ccgcgagcag aggtcggtca gcgacttgaa cgggccgccc tccttgcggg cggcgacgat
     3481 cgactcgatc gcgctctggc cgacgttctt gaccgccagc agcccgaacc ggatcgccgg
     3541 cccctcgacg gtgaactcga cgtcggagtg gtggacgtcg gggagcttga tctcgatgcc
     3601 catccggcgg cattcggcga tcgcggccgc gaccttctcc tcgttcgagc ggaaggccgt
     3661 caggaccgcg gtcatgtact cgaccgtgta gttcgccttc aggtaggccg tctggtaggc
     3721 gatcaacccg taacaggtcg cgtgggcctt gttgaagccg taccgctcga acggctcgaa
     3781 ggccttgaac acagcgtcga taacgcccgg ttccacacct cgctccgcgg cctgggacac
     3841 gaacttgccc ttctgggccc ggaggacgtc cgacttcttc ttgcggatgg catagcccag
     3901 ggtgtccgcc tcgggacccg tgaagccgcc cagcgcgatg gcggcggcca tgatgtcctc
     3961 ctggtagacg aagatcccgt acgtcttctc catgaacggc tgcagcagcg ggtggaggta
     4021 gctcaccggc tccaggccat gcttccggcg gatgtacgcg gggatgtggt ccatgggccc
     4081 cggccggaac agcgccacca tggccgccag gtcatagacc gaggtgggcc gcagctcccg
     4141 gatgtagcgg cgcatgcccg cggactccag ctggaacacg cccgtcgtct cacccgatga
     4201 cagcagctcg aacgtcctgg tgtcgtccag cgggatgtgg tcgatgtcga tgcgctcacc
     4261 accgcgttcc tggaggatga ggtcgaccgc ctggcgcagg atggtcaggt tcgagaggcc
     4321 caggaagtcg aacttcagca ggcccagtgc ctccacgcca tgcatctcgt actgggtcat
     4381 gagcgagtcg gagttggtcg cgcgctggag cggcatgagg tcggtgagcg gctcacggct
     4441 gatcacgacg ccggcggcgt gcgtggacgc gttccgcgcc acgccctcca gctgcttcgc
     4501 gaagtccacg acgcggtgga cggcggggtc gccctcgtac atctcgcgga gctgcgacga
     4561 cgtgacgatg gcctcgtcga gcttgatccc gagctggttg gggaccgcct tggcgatccg
     4621 gtcgatctcg ccgtagctgt ggccgaggac
//
LOCUS       DATAIW010000227         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_11940, whole genome shotgun sequence.
ACCESSION   DATAIW010000227 DATAIW010000000
VERSION     DATAIW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11940"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VHM48_07180"
     CDS             complement(<1..688)
                     /locus_tag="VHM48_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX2755229.1"
                     /translation="MSRARRAGPAALSSALVLALVVASLATAAHRPFPRSSSAASPGG
                     GTAAVAWPASTLLISEIQTGGASASDEFAELANAGPIAVDLIGLELVYATSTGSTVTR
                     KATWASSRILEPGRHLLVANAAGIVAGSADATYSGGFAGTGGAVVLRPVGGSPIDAVA
                     WGDATNAFVEGVAASAPAAGSSIERRPGGASGNGIDTNDNAADFQVATPNPQNLASAP
                     TPDPGASPSPT"
     gene            1065..1856
                     /locus_tag="VHM48_07185"
     CDS             1065..1856
                     /locus_tag="VHM48_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755230.1"
                     /translation="MARAKRTDRAEARRRHRATFADPLAEGDLASTADDAISASAPAR
                     ADGASRARSRSGAATPPARGSTPPPPRPSIPGAFRSSFRPVDLRGDIRALPQIVTHWS
                     LYLSVILAGLSVALVPFLGAGSIAGVMFGYFSGAAPFGTAFIAGLFAPRASYLVGILA
                     ALASAGFMALAFSVGPFGGTLQGMTDANLAPLSESSAKSILLSEALFTGVPLAALVAA
                     AGAWYRRFLNRANPNRARPSNPGGRRPDGKVPKKPQQRPMLARRR"
     gene            complement(2001..4328)
                     /locus_tag="VHM48_07190"
     CDS             complement(2001..4328)
                     /locus_tag="VHM48_07190"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX2755231.1"
                     /translation="MPDALAPRTAIRPGRGDDEGGPGDTPASVAWLRDGRGTGYAVDR
                     ERLVAPALIDDGQGGRRAIDPESGLPESPPRKTDRLGRPNHWDPPAPPTQAMGRGGQS
                     SVARVAPEPPPQARGGTTRDLSDWERWLEFCARIDGFPRHLSIHSGGMLVTAAPLIDI
                     APLERATMPDRVVVQFDKRDVEALKLIKLDLLGLGMLAAIDETLQLIEHDCAVCLDLD
                     RLPEDVAEVFAMLQAADTVGVFQVESRAQMQTLPKSRPSCLDDLVVEVAIIRPGPIQG
                     NAVHPYLRRKQGLEEVTYLHPSLEPVLRDSKGVILYQEQVMRIAIEVAGFTPAASDGF
                     RRAMGTWRSTREMEKLHAQFHDGCMRQPGMTEEVAEELFRQVAAFASFGFAKSHAAAF
                     ARTAYESSFLKLFYPAQFLVGLINAQPMGFYPVEVLVNDAKRHGVAVLPVDINRSTYK
                     TTTEWVGRPGWALGEGDDGPGEALPGDAGIARRPAPVRSSGCLIPSDEARGRWAAESA
                     VGWGVRLGLGLVKGIGEEHEALLDAELEHGSYRSLADVVERTGLPEETLERMIRVGAL
                     DSLGRPRRELLWQLREVAGASRGRLDGRSTKGGAFTRAASRPMALRLPATEAPTLPGL
                     TESERIGDAYAVVGLDARRQVIELYRPALDRLGAVPNAALDDRKPGRVRIGGLVVTRQ
                     HPMTAKGTVFLALEDETGMVNVTLWPDAWARLRGIVRRHALLLVDGELQRESKVVNVI
                     AHEVRPLIEAAASAGAPAQPQGVKQLGMAGMRRMG"
     gene            4611..>4822
                     /locus_tag="VHM48_07195"
     CDS             4611..>4822
                     /locus_tag="VHM48_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755232.1"
                     /translation="MASRSSASDLRERTAGPGRAGRLAPWRHLPDERHPGIQLAHPIH
                     ELLAQVPFDAGCILGPRQSPVAGLSLP"
BASE COUNT          644 a         1818 c         1675 g          685 t
ORIGIN      
        1 gcgtcgggct cggcgatgct cccgggtcgg gcgtcggcgc cgacgcaagg ttctgcggat
       61 tcggcgtcgc aacctggaag tcagcggcgt tgtcgttcgt gtcgatcccg tttccgctcg
      121 ctccgccggg ccgccgttcg atgctcgagc cggccgccgg tgccgacgcg gcaacacctt
      181 cgacgaaggc attcgtggcg tcaccccagg cgacggcgtc gatcggcgat ccgccgaccg
      241 gtcgcagcac gaccgctccg ccggtccctg caaagccccc tgagtaggtc gcatctgccg
      301 atccggcgac gatccccgcg gcgttcgcga cgagcagatg gcgtccgggt tcgaggatcc
      361 gggaggatgc ccaggtcgcc ttccgcgtga cggtcgagcc ggtcgaggtg gcgtagacga
      421 gctcaaggcc gatcaggtcg accgcgatcg ggccggcgtt cgcgagctcc gcgaactcat
      481 cggaagccga tgctccgccg gtctggatct ccgagatcag gagtgtcgac gccggccacg
      541 cgacggcggc ggtccctccc ccgggcgagg ccgcggatga agagcggggg aacggccggt
      601 gggccgccgt cgcaagcgaa gcgacgacca gggcgaggac cagggcagac gacagggcag
      661 caggccccgc ccgtcgggcg cgcgacatcg acgacctccc ggcggggttg tcggaacgag
      721 ggccgcggtt cgaggacggc ccgttggggc ggaacacggt tgtagcaggg gccgcttgtc
      781 gcggcatcga tcgccgctgc accggggatc catcgtccgt ttgcggcggg tcgacactgg
      841 atgcaccgcg gatcaccacc gactgcaggg cgcctgcacc acgtctgcac gacggatcta
      901 cccgagccag cgccgattcc acgggcgtcg acgaccccgc tcgatcgcgc ccggatcgtc
      961 gaacgcgtac gatgctggaa cccgtcgcgt ccacccgacc gccgtaccgg catccgtacc
     1021 cggcccgcgc cctcaaccat gggcgcccat cacctggagg caccgtggcc cgagccaagc
     1081 gcaccgaccg agccgaggcc cgccgacgcc accgcgcgac cttcgccgac ccactcgccg
     1141 agggcgacct cgcgtcgacc gccgacgacg ccatcagcgc gagcgctccg gcgcgcgcgg
     1201 acggcgcctc gcgggcccgc agccggtccg gcgcggcgac gccacccgcg cgcgggtcga
     1261 cacccccgcc gccgcgcccc agcatccctg gcgccttccg gtcgtccttc cgccccgtcg
     1321 acctgcgtgg cgacatccgg gccctgccgc agatcgtcac ccactggtcg ctctaccttt
     1381 cggtcatcct cgcgggcctg tcggtggcgc tcgtcccgtt cctcggcgcg ggatcgatcg
     1441 ccggggtgat gttcggctac ttctcgggcg ccgccccctt cgggaccgcg ttcatcgcgg
     1501 gcctcttcgc cccgcgggcg agctacctgg tcgggatcct cgcggccctc gcgtcggccg
     1561 gattcatggc cctggcgttc tcggtcggac cgttcggagg gacgctccag ggcatgaccg
     1621 atgcgaacct cgcaccgctg tcggagtcct cggccaagtc gatcctgctg agcgaagcgc
     1681 tgttcacggg cgttccgttg gccgcgctgg tcgcggccgc aggcgcgtgg taccgacggt
     1741 tcctgaatcg ggcgaacccg aaccgtgctc ggccgtcgaa cccgggcggc cgccgtcccg
     1801 acggcaaggt cccgaagaag ccccagcagc gcccgatgct ggctcggagg cgatagggct
     1861 gtcgcgcctg gacgacgctg gcggcgttgc cgcctgcggt cctcgcaagc tggcatccga
     1921 gcccgcttgc tgcggtcctc ggggtcgcct tgcgctcgac cacgcatgcg cgacgaacgc
     1981 agtcgccggc tgtccgggcg tcagcccatt cggcgcattc cggccatgcc gagctgcttg
     2041 acgccctggg gctgagcggg cgcgcccgcg ctcgccgcgg cctcgatcag cggccggacc
     2101 tcgtgggcga tgacgttgac gaccttcgac tcccgctgga gctcgccgtc gacgagcagc
     2161 agggcatgac gccggacgat gccgcggaga cgggcccagg catcgggcca cagcgtgacg
     2221 ttgaccatcc cggtctcgtc ctcgagggca aggaagaccg tgcccttggc cgtcatcgga
     2281 tgctgacggg tgaccacgag gccgccgatc cggacccgcc ccggcttccg gtcgtcgagc
     2341 gccgcgttcg ggaccgctcc gagccgatcg agcgccggac ggtacagctc gatcacctga
     2401 cgcctcgcat cgagcccgac gaccgcatag gcatcaccga tccgctcgga ctccgtcagc
     2461 ccgggcaggg tcggcgcctc ggtcgccggc aggcggaggg ccatcgggcg tgacgccgcg
     2521 cgggtgaagg cgccgccctt cgtcgagcgt ccgtcgagcc ggccgcggct cgcgcccgcg
     2581 acctcgcgga gctgccagag aagctcgcgc cgcggccgcc cgagcgaatc gagcgccccg
     2641 acccggatca tccgctcgag cgtctcctcc ggcagcccgg tccgctcgac gacatccgcc
     2701 agcgagcggt acgacccgtg ttccagctct gcgtcgagca gcgcctcgtg ctcctcgccg
     2761 atccccttga ccagcccgag cccgagccgg acgccccagc cgacggcgct ctcggcggcc
     2821 catcgacccc gcgcctcgtc cgacgggatc aggcagccgc tggagcggac cggggccggt
     2881 cgccgcgcga tgcccgcgtc accggggagt gcctcgccgg gtccgtcatc gccctcgccc
     2941 agcgcccagc ccggccgccc gacccactcg gtggtcgtct tgtacgtgct ccggttgatg
     3001 tcgaccggga ggaccgccac cccgtgccgc ttggcatcgt tgacgaggac ctcgaccggg
     3061 tagaagccca tcggctgggc gttgatcagc ccgacgagga actgggccgg gtagaagagc
     3121 ttcaggaacg acgactcgta ggcggtccgg gcgaaggccg ccgcgtgcga cttggcgaac
     3181 ccgaacgagg cgaacgcggc gacctgccgg aagagctcct cggcgacctc ctcggtcatc
     3241 ccgggctggc gcatgcagcc gtcgtggaac tgggcgtgga gcttctccat ctcgcgggtc
     3301 gagcgccacg ttcccatcgc ccggcggaac ccgtcgctgg cagccggcgt gaacccggcg
     3361 acctcgatcg cgatccgcat cacctgctcc tggtacagga tcacgccctt cgaatcccgc
     3421 aggaccggct cgaggctcgg atggagatag gtcacctcct cgaggccctg cttccggcgc
     3481 aggtacgggt ggacggcgtt gccctggatc ggacccggcc ggatgatcgc gacctcgacc
     3541 acgagatcgt cgaggcacga cgggcgcgat ttcggcagcg tctgcatctg ggcccggctc
     3601 tcgacctgga agaccccgac cgtgtcggcc gcctggagca tcgcgaagac ctcggccacg
     3661 tcctcgggca gccggtcgag gtcgaggcag acggcgcagt cgtgctcgat cagctggagc
     3721 gtctcgtcga tcgcggccag catccccagc ccgagcaggt cgagcttgat caacttgagc
     3781 gcctcgacgt cgcgcttgtc gaactggacg acgacccggt cgggcatcgt cgcccgctcg
     3841 agcggcgcga tgtcgatcag cggcgccgcg gtcacgagca tcccgccgct gtggatgctc
     3901 aggtgccgcg ggaagccgtc gatccgggcg cagaactcga gccagcgctc ccagtcggac
     3961 agatcgcggg tcgtgccgcc gcgggcctgc ggcggcggtt ccggcgcgac gcgggcaacg
     4021 ctcgattgcc cgcccctccc catcgcctgg gtcggaggag ccggtgggtc ccagtggttc
     4081 ggccggccca accggtcggt cttgcgtggc ggcgactctg gcagcccgct ctcgggatcg
     4141 atcgctcgcc ggcctccctg cccgtcgtcg atcagcgccg gagcgaccag ccgctcgcgg
     4201 tcgactgcat agcccgtccc gcgcccgtcg cgcagccagg cgacgctcgc cggcgtatcg
     4261 ccgggaccac cctcgtcgtc gccgcggccc ggccggatcg cggtccgagg ggcgagcgcg
     4321 tcgggcaacg ctcgctcccc ccgctccgca ctgatatcga tcccatcggt attccaggga
     4381 ctggatcgac cgaaagccgc gtccggagcg tcgatcgacg gtccggcaaa cgtggccaga
     4441 cggtttgcga gcgtggcagg cgatccgtcg gctcctcgat ctcgcgcctg cgggccgtcc
     4501 aatcccccgg atatcgatgg cgttgatgtg gactcgagga gggctccgac ggcagcgccg
     4561 agcgggatct ccgccagcca gccgaacggc cgtggcgcat cctcgggccg atggcgtcgc
     4621 ggagctccgc ctcggacctc cgagagcgca ccgctggccc gggccgggcc gggcggctgg
     4681 cgccatggcg gcaccttccc gacgagcggc atcctggcat tcagctggcc catcccatcc
     4741 acgagctgct cgcccaggtc cccttcgacg cgggttgcat cctcgggccg cggcaatcgc
     4801 ccgttgccgg cctctccctc cc
//
LOCUS       DATAIW010000228         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_124496, whole genome shotgun sequence.
ACCESSION   DATAIW010000228 DATAIW010000000
VERSION     DATAIW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124496"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VHM48_07200"
     CDS             <1..1605
                     /locus_tag="VHM48_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2755233.1"
                     /translation="TGVALGADAINPVNGERIPIFVADYVLGGYGTGAIMAVPAHDER
                     DYAFALKYGLRIRRVVAAPGDEDRELDIPYLAHSPGERLVDSGPYSGLPADEGGKAIV
                     GELEARGKGKAAVTYRLRDWLISRQRYWGTPIPVIYCERDGIVPVPEEDLPVRLPDTV
                     DYRGSGENPLNRDETFLNVTCPQCGGPAKRETDTMDTFVDSSWYWFRYLSPEKAGAPI
                     EREIVKRWTPVNQYTGGSEHAVMHLLYAREWTKMMRDIGLVEQDEPFKRLFNQGQILG
                     LDGERMSKSRGNTQDPDELVARYGADTIRLFLMFMGPWDQGGPWSPTGIGGVHRFLNR
                     LWTIALDPHGREPGDPDSGSLPAGVDEAAARLQMRQAAHRTLRGVTADYEAFHFNTMI
                     AKLMELGNLLMRFRGSVVAGSEEWDEAVRLSLLMLAPAAPHITEELWSRRLAAAGARW
                     SSIHQETWPAVDEAAVVEATREVPVQVNGKLRDKVVVPADTDAAGLEAAVLASDRIQA
                     VLAGREPLKIIQAGGGKLVNIVVRDA"
     gene            1608..2066
                     /locus_tag="VHM48_07205"
     CDS             1608..2066
                     /locus_tag="VHM48_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755234.1"
                     /translation="MPHVGELRAPRPEPGPGPRPAGVAAVSWGEGRIDRFWIGPEGDL
                     LHAAFDGERWLETESLGGTPASTPGATAWAVDQLQVFAVFADGELWNRYWDGTSWHAW
                     ESLGGVLDPSAGAAASSWSADRIDVWAVGLDGRTWHRLWDGSRWVDWEQL"
     gene            2153..2590
                     /locus_tag="VHM48_07210"
     CDS             2153..2590
                     /locus_tag="VHM48_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755235.1"
                     /translation="MAATVAVGCGAVPRDRSAAAARAEQAIIRPAVDHALADAGVTGH
                     MGDRDRIAFRGHDLTLIEGQLVREDVRSWRTFLYDGSIGTAEQLRFRTDSGSVAFSKP
                     TITAIRVDGAPAILVTLYVISEGAVGAEGGELIYYRTIADGRA"
     gene            2587..3528
                     /locus_tag="VHM48_07215"
     CDS             2587..3528
                     /locus_tag="VHM48_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2755236.1"
                     /translation="MTAIQVGALCWNQYTDWPSLLAAGVRAEDLGYDSLWTWDHLYPI
                     VGSSDGPIHEGWLTLAAWAQATERIRIGLMVGANTFREPALTAKMATTLDHISGGRAT
                     LGIGAAWFEEEHTAYGLEFGDGFPERLRWLAEALPIMRGMLRGETATAKGPHYQARSV
                     RNDPPPIQDRLPLLIGGGGEQVTLKLVARYADANNVGGGIDQVRRKEAVLLRHCETVG
                     RDPAEIERTTGLGVVVIRDSRAKARKASDAIFARNGQADPWKNQPVGTPEDVAAFIAP
                     FLEIGYRHVIAGFPNPYDEESMTRLATDVRPLLGASV"
     gene            complement(3462..4160)
                     /locus_tag="VHM48_07220"
     CDS             complement(3462..4160)
                     /locus_tag="VHM48_07220"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX2755237.1"
                     /translation="MATSARAFVRRHTRLRPVPGLEGIRLHLADEVLPLWHAVQVETN
                     DPDAALPYWAFAWGGGLAIAHYLAEHPEAVAGRRVFDLASGSGLCAVAASLAGASSAT
                     AADIDAFAAAAIELNTRANGHRVAVVARDILDEEPPDADVILAGDVCYEAGLAERVLP
                     WLQRARARGLDVLLGDPGRRYLPASELVELARYDVRTTTELEDLELKKGWVYTLAPSS
                     GRTSVASRVIDSSS"
     gene            complement(4170..4823)
                     /locus_tag="VHM48_07225"
     CDS             complement(4170..4823)
                     /locus_tag="VHM48_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX2755238.1"
                     /translation="MNATAIAHRPATSDHVHDAVPLETAFDFLNTLELENGALVERLT
                     SLDTAATWLADAAVVPDATAITAVGRSAVDREAALRRLVTARTALRDVAHAVAHEEAP
                     PRAAIDEVNRALAGRERVELVPADDGVRIGHSHVGDAIDHVLARIAEPIVREIGSGHD
                     DRVRICANDTCRWIFYDESRAGRRRWCDMATCGNRAKARRHRARRKETGEVEAADAR"
     gene            5084..5287
                     /locus_tag="VHM48_07230"
     CDS             5084..5287
                     /locus_tag="VHM48_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755239.1"
                     /translation="MQSNYYAFLALELARERVAEANAQRLAALAHPGEPWTAGIRRAI
                     ARIALAVARAADDEVGRVPLTTH"
     gene            5457..6200
                     /locus_tag="VHM48_07235"
     CDS             5457..6200
                     /locus_tag="VHM48_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2755240.1"
                     /translation="MDEPAPWRALEDHPPAAGNGATGPVPLTDPGGASATRIPWLVAA
                     FATAAVVAGIAIWLVASSGRGSVVVEGGGPAPGGSARDRGAIAIAAAASTGPALIVDV
                     QGAVVRPGIVRLPAGARVADAIAAAGGFGPRVAADRVGTMLNLAALVKDGDQVIVPSR
                     DDPAAAPTPGTTGAGGPGGATTGAGPLDLNRATAAELDALPGIGPVTAAKIIAARGEQ
                     AFASVDDLRSRKILGAATFDKVKDLLVVR"
BASE COUNT          900 a         2228 c         2344 g          863 t
ORIGIN      
        1 accggcgtcg cgctcggcgc cgacgcgatc aacccggtca acggcgagcg gatcccgatc
       61 ttcgtcgccg actacgtgct cggcggctac gggaccggcg cgatcatggc cgtccccgct
      121 cacgacgaac gcgactacgc gttcgcgctg aagtacggcc tgcggatccg gcgggtcgtc
      181 gcggctcctg gcgacgagga ccgcgagctc gacatcccgt acctcgccca ttcacccggc
      241 gagcggctgg tcgacagcgg accgtacagc gggctgccgg ccgacgaggg cggcaaggcg
      301 atcgtcggcg agctcgaggc acgcggcaag ggcaaggccg ccgtcacata ccgcctgcgc
      361 gactggctga tcagccggca gcgctactgg ggaacgccga tcccggtgat ctactgcgag
      421 cgcgacggga tcgtgccggt gcccgaggaa gacctcccgg tccggctgcc cgacaccgtc
      481 gactaccgcg gcagcggcga gaacccgctc aatcgcgacg agacattcct caacgtgacc
      541 tgtccgcagt gcggcggccc cgcgaagcgc gagaccgaca cgatggacac gttcgtcgac
      601 tcatcgtggt actggttccg ctacctgtcc ccggagaagg ccggtgcgcc gatcgagcgg
      661 gagatcgtca agcgctggac gccggtcaac cagtacacgg gcggctccga gcacgcggtc
      721 atgcatctgc tctacgcccg ggagtggacg aagatgatgc gcgacatcgg tctcgtcgag
      781 caggacgagc cgttcaagcg cctgttcaac cagggccaga tcctgggcct ggacggcgag
      841 cggatgtcga agtcgcgcgg caacacgcag gacccggacg agctcgtcgc acggtacggc
      901 gccgacacga tccgcctgtt cctgatgttc atgggcccct gggaccaggg cgggccgtgg
      961 agcccgaccg ggatcggcgg cgtccatcgg ttcctgaacc ggctctggac gatcgcgctc
     1021 gaccctcacg gccgcgagcc cggcgacccg gacagcggct cgctgccggc cggcgtcgac
     1081 gaggccgcgg cccgcctcca gatgcgccag gcggcgcatc ggaccctgcg cggggtgact
     1141 gcggactacg aggcgttcca cttcaacacg atgatcgcca agctcatgga gctcgggaac
     1201 ctcctgatgc gcttccgggg atccgtcgtc gccgggtccg aggaatggga cgaggccgtt
     1261 cgcctgtcgc tgctgatgct ggccccggcc gccccgcaca tcaccgagga gctctggtcg
     1321 cggcggctgg ccgcggccgg cgcgcggtgg tcgtcgatcc atcaggagac atggccggcc
     1381 gtggacgagg cggccgtcgt cgaggccacc cgcgaagtcc cggtccaggt caacggcaag
     1441 ctccgcgaca aggtcgtcgt cccggccgac actgacgccg ccgggctcga ggcggccgtc
     1501 ctcgccagcg accggatcca ggccgtcctc gccggacggg agccgctgaa gatcatccag
     1561 gccggcggcg gcaagctcgt caacatcgtc gtccgggacg cctgaccatg ccccacgtcg
     1621 gtgagctccg ggctccccgc cccgaacccg gacccggccc gcgcccggcc ggggtcgccg
     1681 ccgtgtcgtg gggcgagggg cggatcgacc ggttctggat cgggcccgag ggcgacctgc
     1741 tccacgcggc gttcgacggc gagcgctggc tcgagaccga gtcgctcggc gggacgccgg
     1801 cctcgacgcc gggcgcgacc gcgtgggccg tcgaccagct ccaggtgttc gccgtcttcg
     1861 ccgatggcga gctgtggaat cgctactggg acgggacgag ctggcacgcg tgggagtcgc
     1921 tcggcggtgt gctggacccg tcggcgggcg cagcggcgtc gtcgtggtcg gccgaccgga
     1981 tcgacgtctg ggcggtcggg ctcgacggcc ggacatggca ccgcctttgg gacggcagtc
     2041 gttgggtcga ctgggagcag ctctgaccgt ccccgctccg gtcgagcgtc atgacaggga
     2101 gacaggacac ccggctgtgc atgcggacca tcgggctctg acgttgctgg cgatggccgc
     2161 gaccgtcgcg gtcgggtgcg gagcggtgcc gcgcgaccgg tcggccgcgg cggcgcgggc
     2221 cgagcaggcg atcatccggc cggcggtcga ccacgcgctg gccgacgccg gagtgacggg
     2281 tcacatggga gatcgcgatc gaatcgcctt ccgtggtcac gacctcacgc tgatcgaagg
     2341 ccagctcgtc agggaggatg tccggtcgtg gcggacgttc ctctacgatg gatcgatcgg
     2401 gaccgcggag cagctgcgtt tccggaccga ttccggcagc gtcgcgttct cgaagccgac
     2461 gatcacggcg atccgcgtcg atggcgcgcc ggcgatcctg gtcacgttgt acgtcatctc
     2521 tgaaggcgcg gtgggcgcgg agggcggtga gctgatctac taccggacga tcgcggacgg
     2581 tcgcgcatga ccgccatcca ggtcggcgcg ctgtgctgga accagtacac cgactggccg
     2641 tccctcctcg cggccggcgt ccgcgccgag gacctcggct acgacagcct ctggacgtgg
     2701 gaccacctct acccgatcgt gggcagcagc gacggcccga tccacgaggg ctggctgacg
     2761 ctggcggcgt gggcccaggc gaccgagcgg atccggatcg ggctgatggt cggcgcgaac
     2821 acgttccgcg agccggctct gaccgcgaag atggcgacga ccctcgacca catctcgggt
     2881 ggtcgggcga cgctcgggat cggcgcggcg tggttcgagg aggagcacac cgcctacggc
     2941 ctcgagttcg gcgacggctt cccggagcgg ctgcgctggc ttgcggaggc gttgccgatc
     3001 atgcgcggga tgctccgcgg cgagacggca accgccaagg gcccgcatta ccaggcgcgg
     3061 tcggtccgca acgacccgcc gccgatccag gaccggctgc cgttgctgat cggtggtggc
     3121 ggcgagcagg tgaccctcaa gctcgtcgcc cggtacgccg acgcaaacaa tgtcggcggc
     3181 gggatcgacc aggtccgccg caaggaggcg gtcctcctcc gtcactgcga gaccgtcggg
     3241 cgcgatccgg ccgagatcga gcgcacgacc gggctcggcg tcgtggtgat ccgcgactcg
     3301 cgcgcgaagg cccgcaaggc ctccgacgcg atcttcgccc ggaacggcca ggcggacccg
     3361 tggaagaacc agccggtcgg gaccccggag gatgtcgcgg cgttcatcgc cccgttcctc
     3421 gagatcggct atcgccatgt gatcgcgggc ttcccgaacc cctacgacga ggagtcgatg
     3481 acccggctcg cgaccgacgt ccggccgctg ctcggcgcga gcgtgtagac ccagcccttc
     3541 ttgagctcca ggtcctcgag ctcggtcgtc gtccggacgt cgtagcgggc cagctcgacg
     3601 agctcgctcg cgggcaggta gcggcggccc gggtcgccga gcaggacatc gagcccgcgg
     3661 gcacgcgccc gctgcagcca gggaaggacg cgctccgcga ggccggcctc gtagcagacg
     3721 tcgccggcga ggatcacgtc ggcatccggt ggctcctcgt cgaggatgtc gcgggcgacg
     3781 actgcgaccc gatggccgtt ggctcgcgtg ttgagctcga tcgccgcggc cgcgaaggca
     3841 tcgatgtcgg cggcggtcgc gcttgacgcg ccggcgagcg acgccgcgac cgcgcagagc
     3901 ccggagccgg aggccaggtc gaagacgcga cgcccggcga cggcctccgg gtgttcggcg
     3961 aggtagtggg cgatcgccag cccgcctccc caggcgaacg cccagtacgg cagggcggcg
     4021 tcgggatcgt tcgtctcgac ctggacggcg tgccacagcg gcagcacctc gtcggccagg
     4081 tggaggcgga tcccctcgag cccggggacc ggccgaaggc gcgtgtggcg gcggacgaag
     4141 gcgcgggcgg aggtggccat ggccgcactc tagcgagcgt cggccgcttc gacctccccg
     4201 gtctccttcc ggcgggcccg atggcgccgg gccttggccc ggttgccgca ggtcgccatg
     4261 tcgcaccagc ggcggcggcc cgcccgcgac tcgtcgtaga agatccagcg acacgtgtcg
     4321 ttggcgcaga tccggacccg gtcgtcgtga ccgctgccga tctcgcggac gatgggttcc
     4381 gcgatccggg cgaggacgtg gtcgatcgcg tcgccgacgt ggctgtggcc gatccggacg
     4441 ccgtcgtcgg cgggaacgag ctcgacccgc tcgcgaccag cgagggcgcg attgacctcg
     4501 tcgatcgcgg cacgcggcgg cgcctcctcg tgggcgaccg cgtgggccac gtcgcggagg
     4561 gcggtccggg cggtgacgag gcggcggagg gcggcttcgc gatcgaccgc ggagcgcccg
     4621 acggccgtga tcgcggttgc gtcaggcacg acggcggcat ccgccagcca ggtcgccgcc
     4681 gtatcgagcg aggtcaaccg ctcgacgagt gcaccgttct cgagctcgag cgtgttcagg
     4741 aaatcgaagg ccgtctcgag ggggaccgcg tcgtggacgt ggtcgctcgt cgcgggcctg
     4801 tgggcgatgg cggtggcgtt cacctaacca ccctatatcg cttgacaggt tacgcgcaag
     4861 aggctaaggt acgaagtaac cacaacaaca cgagacaccg gttacgacac cggagacggg
     4921 agagtccgat ggacgccttg atcgccctcg ccgccatcct cgccggcctc atcgggctcg
     4981 acatcgccgc cctgtgctgg ggcaccgaca gccgcgactc gttcatcgat gaccacgtcc
     5041 gctgaccggc cgacaccgcc ggttcgctcc aggagaacat gacatgcagt ccaactacta
     5101 cgccttcctc gccctcgagc tcgcccgtga gcgcgtcgcc gaggcgaacg cccagcggct
     5161 ggctgctctc gcccacccgg gcgagccgtg gacggccggg atccggcgcg cgatcgcgcg
     5221 gatcgccctg gccgtcgccc gggcggccga cgacgaggtc gggcgggtcc cgctgacgac
     5281 ccactgacgc gtgtccggat ccgcgatacg cgagtgcagc taagcggcag ataagtcgcg
     5341 acggctaagg tgccgccaga cgccccgccg acgtcggaag tgcccccaac gtcgcggggc
     5401 gtcgagctgt ccgccacggc tcgtcggtcc cgcgctcctc ccgccgagga ggaacgatgg
     5461 acgaaccggc gccgtggcgc gccctcgagg atcacccgcc ggccgcaggc aacggcgcca
     5521 ccgggccggt tccgctgacc gatccgggag gtgcgagcgc gacccgcatc ccgtggctcg
     5581 tcgcagcgtt cgcgacggcg gcggtcgtcg ccgggatcgc gatctggctc gttgcgtcga
     5641 gcggccgggg ttcggtcgtc gtcgagggcg gcggcccggc ccccggcgga tccgcccgtg
     5701 atcgtggcgc gatcgcgatc gctgcggcgg cgtcgaccgg gccggccctc atcgtggacg
     5761 tccagggtgc cgtcgtgcgg cccggaatcg tccgcctgcc agccggagcc cgcgtcgccg
     5821 acgcgatcgc ggcggccggt ggcttcgggc cacgcgtggc cgcggaccgc gtcggcacga
     5881 tgctgaacct ggcggccctc gtcaaggacg gcgaccaggt gatcgtgccg tcgcgcgacg
     5941 acccggcggc tgcgccgaca cccggaacga cgggcgccgg agggccggga ggcgcgacga
     6001 ccggcgccgg acctctggac ttgaatcggg cgacggcagc cgagctcgat gcgctgcccg
     6061 ggatcgggcc ggtcacggcc gccaagatca tcgcagctcg cggcgaacag gcgttcgcgt
     6121 cggtcgacga cctgcggagc cggaagatcc tcggcgccgc gacgttcgac aaggtcaagg
     6181 acctcctcgt cgtccgatga gcgcccgacg tcggcggctt tcgggggatc gatcgtgatc
     6241 gcgagcggct ggctggcgat cggggccgtc ctcgccgcgc tcgtcgcgcc gggcgtcgga
     6301 tcgccggtcg tggccatcgc ggcggcggcc accgc
//
LOCUS       DATAIW010000229         6960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_243689, whole genome shotgun sequence.
ACCESSION   DATAIW010000229 DATAIW010000000
VERSION     DATAIW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243689"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2634
                     /gene="ligD"
                     /locus_tag="VHM48_07240"
     CDS             <1..2634
                     /gene="ligD"
                     /locus_tag="VHM48_07240"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX2755241.1"
                     /translation="TGRFVVQRHRATRLHYDVRLEVDGVLVSWAVPKGPTLDSAVRRM
                     AVHVEDHPIEYFDFEGVIPHGQYGAGDVIVWDWGTWTPEAETPDPVAAIERGELKFVL
                     AGQKVRGRFTIVRTSRRPGSAPTTAFEDDSEQWLLIKKRDAAAVPGWDAEDYPQSVRT
                     GRTNDEVKANRDAIWISEQPAAIAGIDLAGAVERAMPDRIEPMLATLATKAFDDPDWL
                     FEIKWDGYRVQAVVRDGKARLWTRNLKDAETYFPNLLSPPTWIDAREAIVDGEVVALD
                     ADGRPDFSLLQVRISQRRGTATTNGPAAGALVYQVFDLLYLDGRSLLGVPLEDRKRLL
                     RSVLRETARVRYASHVVGDGEAFMAAAKSRGLEGIVAKLRRSRYEPGKRTPAWLKLKI
                     RPEQELVVGGWTPGEGNARDLGAVAVGVYEEGKLRFAGKVGSGFTAATRKRLLAAMAP
                     LVAEAPPFDPPPPRDYRGRWGGDLNGVTWIRPELVIRAELGGWTRDGHVRQTSFKGIE
                     PGRDPRLVVREEAVSSARAVAEAEASEPESGENRPMSTKKGSRATAFPDAPEAAELER
                     LATMPAEGVWHVGGERGDDLKLTNLDKVLFGPPPSDPGAPPITKRELIAYFARIAPAM
                     LPHLAERPLNLQRFPNGAGGPSFWQKDIPTSAPGWLRRWHEVGVDDRKANDHLIADRV
                     ATLCWLGNQAAFEVHAWTARLEDPFRPTFALIDIDPGSKTTWDETLVLARLYRTALAH
                     LGVRGYPKTTGKRGIQVWIPIEPRYTFADTSGWVEKISRAIGQTVPDLVSWDWAKANR
                     GGKARLDYTQNATIKTLVAPYAVRPSPGAPVSAPISWDELDEPDLRPDGWTIRTIVER
                     VERVGDLFAAAQTDAQVLPAV"
     gene            complement(2651..3322)
                     /locus_tag="VHM48_07245"
     CDS             complement(2651..3322)
                     /locus_tag="VHM48_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755242.1"
                     /translation="MNERTVPEFRPSTHGFHFANAWPPGPTVRFGPLDPRIVGVGDAS
                     TGLCGGMVYTIADLYAAGIPVPPDREPPANGSPRFASIVRRQVQSLAFLTVPLRFWLR
                     MALGSSFGRDRARSTFDREWPKARAWLDAGRLLPIGLIRVASTNPRNLTKNHQVIAWG
                     YAEDGRGVTLRIYDPNWPDRDDVAITIHLDNALRPGGLSQATGESLLGWFVLPYSPAD
                     PRAWR"
     gene            complement(3365..5233)
                     /locus_tag="VHM48_07250"
     CDS             complement(3365..5233)
                     /locus_tag="VHM48_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755243.1"
                     /translation="MRELQIGVSRHSNSGRIGRARFALVAGLAVLALGGGPLVAPVEA
                     APSQPAAAGGAAPKVVLIVGPAGGATPYYRRLADEAAAAALKLTPNVVKVYSPDATWT
                     NVKAALQGASVVVYLGHGNGWPSIYRDALYPVTQDGFGLNPHAGAADSHQYFGEKQIG
                     DEIKLAPDAVVIFSHLCYASGNTEPGLAEGTLDQAQQRVDNYAAGFFRAGAGAVIADA
                     YLAPAYYVRSVLRGTRSIDAIWKNAPNHNDHFLRFASQRTKGAIAQMDPDAVDSGFHR
                     SIVLKAKLTSAEVIGNAVRGPRTPIPVLEPTLAGLGMTFGAPDLTTPPTAGSTTTLTL
                     PVASEAAALLPAKLMVGIRWDRLDAGAATDPATGAGTAPAPPAPSPAPSSGSPTPKPA
                     AGGTGTATVGGDDPGTTAPDVPAIDLVVPEQPGEVVAPVAAKRLRTGGISVRVTVPST
                     PGLYRLVATVHQPDGVAYDAATQALVPALVVRVTGPISATYGAPTTATAVAGASFALN
                     VRVTNLGRSAWGHPAQSQSVSAAELEPASRARLVARWVDLGGAAAAPVPPAGGVVNSI
                     LPAGLAPGASVNAAFVLTAPAAPGEYLLVLDVVDPVTGSLAAAGVPPGIVRVTVTR"
     gene            5552..6208
                     /locus_tag="VHM48_07255"
     CDS             5552..6208
                     /locus_tag="VHM48_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755244.1"
                     /translation="MTRQRLIGTLLVTAMVAACGSTATPAPSSNAGPSASDVVSAAAS
                     LPPSPSPSPSPSPTPTPSPTPTLAPTPAPTPVPWKTYTSKRFHYTMKYPPDWVVTPGS
                     ATLSDQYDNFGYPYIYVTRDLVSTSVSISRTVTFEISYFKSHYHAKLISSQAIKLAGY
                     SGRLLTFSGTDNGLKVIIKEVIVGKGKVGYFITMFGNATAAAADRTLFRKMYLTWRPT
                     "
     gene            6305..6835
                     /locus_tag="VHM48_07260"
     CDS             6305..6835
                     /locus_tag="VHM48_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2755245.1"
                     /translation="MSQPDPSTATVVEPRLRRIHEVAAELGLTTRTIRYYEELGLLEP
                     AGRSGGDYRQYDADDVERLRFIKGLRDDAGFSLAEIGRLLEDEAARERNRARYRTTSD
                     ATERRAIVDDALARVERQIETLRDKMSRLAAMIDEAEDRRTHLLIHNAGLEAGVPPAP
                     HKHGRPVGTAVAGTDR"
BASE COUNT         1075 a         2414 c         2497 g          974 t
ORIGIN      
        1 acgggccgtt tcgtcgtcca gcgccaccgc gcgacgcggc tccactacga cgtccggctc
       61 gaggtcgacg gcgtcctcgt cagctgggcc gtgccgaagg gcccgaccct cgactccgcg
      121 gtccgacgga tggcggtcca cgtcgaggat cacccgatcg agtacttcga cttcgagggc
      181 gtcatcccgc acggccagta cggggccggt gacgtcatcg tctgggactg gggaacctgg
      241 actcccgagg cggagacgcc cgatccggtc gccgcgatcg agcgcggcga gctgaagttc
      301 gtcctcgccg gccagaaggt ccgtggccgg ttcacgatcg tccggacgag ccgccgcccg
      361 ggcagtgcgc cgacgaccgc gttcgaggac gacagcgagc agtggctcct gatcaagaag
      421 cgcgatgcgg cggctgtgcc cggctgggac gccgaggatt acccgcagag cgtccggacc
      481 ggccggacga acgatgaggt caaggccaat cgtgacgcga tctggatcag cgagcagccg
      541 gccgcgatcg ccgggatcga tctcgcgggc gcggtcgagc gggcgatgcc ggaccggatc
      601 gagccgatgc tcgcgaccct cgcgacgaag gccttcgacg acccggactg gctgttcgag
      661 atcaagtggg atggctatcg cgtccaggcc gtcgtccgcg acgggaaggc aaggctctgg
      721 acccgcaacc tcaaggacgc cgagacgtac ttcccgaacc tgctttcgcc gccgacctgg
      781 atcgacgccc gcgaggcgat cgtggacggc gaggtggtcg ccctcgacgc cgacgggcga
      841 ccggatttct ccctgctcca ggtacggatc tcgcagcgac gcgggacggc gaccacgaac
      901 gggcctgccg ccggagcgct cgtctaccag gtgttcgatc tcctgtatct cgacggtcgg
      961 tcgctcctcg gcgtcccgct cgaagaccgc aagcgcctgc ttcggagcgt gctccgggaa
     1021 accgctcggg tccgctatgc atcgcacgtc gtcggcgatg gtgaggcgtt catggccgcc
     1081 gcgaagagcc gcggcctcga ggggatcgtg gccaagctcc gtcgctcgcg ctacgaaccc
     1141 ggcaagcgga ccccggcctg gctgaagctg aagatccggc cggagcagga gctcgtcgtc
     1201 ggcggctgga cgccgggcga gggcaatgcc cgcgatctcg gggcggtcgc cgtcggcgtg
     1261 tacgaagagg gcaagctccg gttcgcgggc aaggtcgggt cggggttcac ggccgcgacc
     1321 cgcaagcgcc tcctcgccgc gatggcgccg ctcgtcgcgg aggccccgcc gttcgacccg
     1381 ccgccgccgc gcgattaccg cggccgctgg ggcggcgacc tcaacggggt cacctggatc
     1441 cggccggaac tcgtgatccg ggccgagctc ggcgggtgga cccgcgacgg ccacgtccgc
     1501 cagacctcgt tcaaggggat cgagccgggg cgcgatccgc ggctcgtcgt gcgcgaggaa
     1561 gcggtctcgt cggcgcgtgc agtggccgag gcggaagcga gtgagcccga gtcgggggag
     1621 aatcggccga tgagcacgaa gaagggatcc cgcgcgaccg catttccgga tgcgcccgaa
     1681 gccgctgagc tcgagcgact cgcgacgatg ccggccgaag gcgtctggca cgtcgggggc
     1741 gaacgcggcg acgacctcaa gctgacgaac ctcgacaagg tcctgttcgg gccgccgccg
     1801 tccgatccag gtgcgccgcc gatcacgaaa cgcgagctga tcgcctactt cgcccggatc
     1861 gcgccggcga tgctgccgca cctcgcggag cgcccgctca acctccagcg gtttcccaac
     1921 ggcgccgggg gcccgagctt ctggcagaag gacatcccaa catcggcgcc gggctggctc
     1981 cgccgctggc acgaggtcgg cgtcgatgac cgcaaggcga acgaccacct catcgccgat
     2041 cgggtggcga ccctgtgctg gctgggcaac caggccgcgt tcgaggtcca cgcctggacc
     2101 gcccgcctcg aggacccgtt ccgcccgaca ttcgccctga tcgacatcga tccggggtcg
     2161 aagacgacct gggacgagac cctggtcctc gcccggctct accggacggc tctcgcccat
     2221 ctcggcgtcc gcggctatcc gaagacgacg ggcaaacgcg ggatccaggt ctggatcccg
     2281 atcgagcccc gctacacgtt cgccgacacg agcggctggg tcgagaagat ctcgcgggcg
     2341 atcggccaga cggtcccgga cctcgtctcg tgggactggg cgaaggcgaa ccgtggcggc
     2401 aaggcccgtc tcgactacac ccagaacgcc acgatcaaga cgctcgtcgc gccgtacgcc
     2461 gtgcgtccct cgcccggggc gccggtctcg gcgccgatca gctgggacga gctcgacgag
     2521 ccggacctgc ggccggacgg ttggacgatc cggacgatcg tcgagcgggt cgagcgggtc
     2581 ggggacctgt tcgcggccgc ccagacggac gcccaggtcc tgccagcggt ctgacctgcg
     2641 ggaccgcccg tcagcgccag gcgcgcggat ccgccggcga gtacgggagg acgaaccagc
     2701 cgaggagcga ctcgcccgtg gcctggctca ggccgccggg gcgcagtgcg ttgtcgaggt
     2761 ggatcgtgat cgcgacgtcg tcgcggtccg gccagttcgg gtcgtagata cggagggtca
     2821 ccccccgccc gtcctcagcg tagccccagg cgatcacctg gtggttcttc gtgaggttcc
     2881 gcgggttggt cgacgcgacc cggatcagcc cgatcgggag gaggcggccc gcgtcgagcc
     2941 aggcccgtgc cttcggccat tcgcgatcga acgtcgagcg ggcgcggtcg cggccgaacg
     3001 agctgccgag ggccatccgc agccagaacc ggagcggcac ggtcaggaac gcgagcgact
     3061 ggacctggcg gcgaacgatc gaggcgaagc ggggtgagcc gttggccggc ggctcgcggt
     3121 ccggcggaac cgggatgcct gcggcgtaca ggtcggcgat cgtgtagacc atgccgccgc
     3181 acaggccggt cgaggcgtcc ccgaccccga cgatccgcgg atcgagcggc ccgaaccgga
     3241 ccgtcggccc cggcggccac gcgttcgcga agtggaatcc gtgggtgctc ggcctgaact
     3301 cggggacggt ccgttcgttc acgctggaaa tcctagaccc gcgcggtcgg cggcgcgttc
     3361 ggcgctatcg ggtcacggtc acccggacga tgccgggcgg cacgcctgcc gcggccagcg
     3421 acccggtcac ggggtcgacg acgtcgagga cgaggaggta ctcgccgggc gcggcaggcg
     3481 ccgtcaggac gaacgcggcg ttgaccgatg cgccgggcgc caatcctgcc ggcaggatgg
     3541 agttcacgac gccgccggct ggcggtaccg gcgccgccgc ggcgccgccg aggtcgaccc
     3601 aacgggccac cagcctggcg cgactggcgg gctcgagctc ggccgcgctc accgactggg
     3661 actgggccgg atggccccac gcgctgcggc ccagattggt gacccggacg ttgagcgcga
     3721 acgaggcgcc ggcgacagcg gtcgcggtgg tcggcgcccc ataggtcgcc gagatcggac
     3781 cggtcacccg gacgacgagg gccgggacga gcgcctgggt cgcggcgtca tacgcgacgc
     3841 cgtcgggctg gtggacggtt gccacgagcc gatacaggcc aggggtcgac ggaacggtca
     3901 cccgcaccga gatcccgccg gtccgcaggc gcttcgccgc gaccggggca acgacctcgc
     3961 cgggctgttc gggcacgacg agatcgatcg ccggaacgtc gggggcggtc gtgcctggat
     4021 cgtcaccgcc gaccgtcgcg gtccccgttc cgcccgccgc gggcttgggt gtcggcgacc
     4081 ccgatgacgg tgccggtgac ggtgccggtg gggcgggcgc cgtgcccgcg cctgtcgccg
     4141 gatccgtggc cgcgccggca tcgaggcggt cccagcggat gccgaccatc agcttggccg
     4201 gaaggagggc ggctgcctcg ctcgccacgg gcagggtcaa cgtcgtcgtc gagccggcgg
     4261 tcggtggcgt cgtcaggtcg ggcgcgccga acgtcatgcc gagccctgcc agggtcggct
     4321 cgagcaccgg gatcggcgtc cggggcccgc ggacggcatt gccgatgacc tcggcgctcg
     4381 tcagcttcgc cttgagcacg atcgaacggt ggaacccgga gtcgaccgcg tcgggatcca
     4441 tctgggcaat cgcacccttc gtccgctggc tggcgaaccg caggaagtgg tcgttgtggt
     4501 tcggcgcgtt cttccagatc gcgtcgatcg agcgcgtccc gcggaggacg gatcggacgt
     4561 agtacgccgg cgcgagatag gcatcggcga tgaccgcgcc ggccccggcc cggaagaacc
     4621 cggccgcata gttgtcgacg cgttgctgag cctggtcgag ggttccctcg gcgaggccgg
     4681 gctccgtgtt gccgctcgcg tagcagaggt ggctgaagat cacgacggcg tcgggcgcga
     4741 gcttgatctc gtcgccgatc tgtttctcgc cgaagtactg gtgcgagtcg gcggcgcccg
     4801 cgtgcggatt cagcccgaac ccgtcctggg tcacggggta gagggcatcg cggtagatgc
     4861 tcggccagcc gttgccgtga ccgagataga cgacgaccga cgctccctgg agggcggcct
     4921 tgacgttcgt ccaggtcgca tcgggcgagt agaccttgac gacgttcggg gtcagcttca
     4981 gcgcagccgc agcggcctca tcggccagcc gccggtagta gggagtcgcc ccgcccgccg
     5041 gcccgacgat gagcacgacc ttgggagcgg ccccgccggc cgcagccggc tgtgacgggg
     5101 ccgcctcgac cggcgcgacc agcggtccac cgccgagcgc gagcacggcg aggccggcca
     5161 cgagcgcgaa gcgagcacgg ccgatccggc cgctgttcga atgtcgagac acgccgatct
     5221 gcaactccct caagtcgact gaacgatagt cctcaggatg cgagcggacc ataacaggag
     5281 aacattcagt ttccgttacc cgaaggttcg cccgcgctca tcggtgactt tgcgcaccac
     5341 caacctggtc gccggatcga cggagtcatt caccggcgag ggcattccga tggacgtgga
     5401 ccggccggtc ttgcgcatgg acagcgaccc atccagactg gacctgccga gcccacggcg
     5461 cctgcagcca acgaccaggc cgaactcgaa gcgtgacgaa ctgcgccttg accgagaggt
     5521 cggggccaag caccggggag gaggaggggt catgactcga caacggctga tcggcacgtt
     5581 gctcgtcacc gccatggtcg ccgcgtgcgg gagcaccgcg acgcccgctc cctcgtcgaa
     5641 tgccggaccg tcggcgtccg acgtcgtcag cgccgccgcg tctctgccgc cttcgccgtc
     5701 gccctcgccg tcaccaagcc cgactccgac cccgtcgccg acgccgacgc tcgccccgac
     5761 gcctgctccg acgccggtcc cctggaagac ctacacgtcg aagcgcttcc actacacgat
     5821 gaagtacccg cccgattggg tcgtgacgcc cggatccgcg acgctgtccg accagtacga
     5881 caacttcggg tatccgtaca tctacgtgac gcgcgacctc gtgtcgacgt cggtgtcgat
     5941 cagccggacc gtcaccttcg agatctcgta cttcaagagc cactaccacg cgaagctgat
     6001 ttcgagccag gcgatcaagc tcgccgggta ttcgggccgg ttgctcacct tcagcgggac
     6061 cgacaacggc ctgaaggtga tcatcaagga ggtgatcgtg ggcaagggca aggtcggcta
     6121 cttcatcacg atgttcggca acgcgacagc cgcggctgcc gaccggaccc tgttccggaa
     6181 gatgtacctg acgtggaggc cgacgtaaac ctgggtgatc ggactggcgt tgatggcgtc
     6241 gagagcggcg tcgcacgcag gctaaccttt acgtcaacgg caaggttgtg gtagactcgg
     6301 ggggatgagc cagcccgacc cctcgaccgc gaccgtggtc gagccgcgtc tccgtcgcat
     6361 ccacgaagtc gccgcggagc tcgggctcac gacgcgcacg atccgctact acgaggagct
     6421 ggggctgctc gagccggccg gtcggtcggg cggcgactac cggcagtacg acgcggacga
     6481 tgtcgagcgg ctgcgcttca tcaagggcct gcgcgacgat gccggcttca gcctcgccga
     6541 gatcggccgg ctcctcgagg acgaagcggc ccgcgagcgg aaccgggcgc gctatcggac
     6601 gacctccgac gcgaccgaac gccgggcgat cgtcgacgac gctcttgccc gggtcgagcg
     6661 ccagatcgag acgctgcgcg acaagatgag tcgcctcgcc gccatgatcg acgaggccga
     6721 ggatcgtcgc actcatcttc tgatccacaa tgccgggctc gaggccggcg tcccaccggc
     6781 gccccacaag catggtcggc cggtcggcac ggcggtcgcc ggaaccgatc gatgacgatc
     6841 tcgtttcgtg gcgcgcgggc cctccgccac cggaactacc ggctgttctt cgcgggccag
     6901 gcgatctcgc tcgtcgggac gtggatgcag caggtcgccc agggctggct cgtcctccag
//
LOCUS       DATAIW010000230         8372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_42304, whole genome shotgun sequence.
ACCESSION   DATAIW010000230 DATAIW010000000
VERSION     DATAIW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42304"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VHM48_07265"
     CDS             complement(<1..995)
                     /locus_tag="VHM48_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2755246.1"
                     /translation="MTAPAITLAQPRRRRRFSPDTRTALFYLLPAFLVMGVITFYPLL
                     FQTWMSFTDFGLKNLSGKVAPNLVGADNYVQIVQSKLNIPNFEFLRLLFFNLFWAFSN
                     VVIHVILGVGIAILLNTNGLRFKKVYRALFILPVIIPPIIVATVWRHLFDQDNGAINM
                     VLQGIGSLFKIPPDAFRIDWLGQVDDPIPFLPLPLAYFALLTANTWLGWPLNSVVATG
                     ALQSVPPDLYEAAEMDGANAWQRFKVVTLPFLRPAMLPYAIYGFVVTFNLFYLSYFMS
                     NGGPFGRTELFVTEAYKLVNDFQLYGVAAAFSVILFFLLLGITLVTNRLARATAS"
     gene            complement(1124..2386)
                     /locus_tag="VHM48_07270"
     CDS             complement(1124..2386)
                     /locus_tag="VHM48_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2755247.1"
                     /translation="MNTKLRVLSTLGALAIVVGACSSGATPTPAGSTGTGASQPAPST
                     AGGSTAPATGLKGALTVWASYGSGAGTEPTAFKTLTDKISAANPDLKLTIQDIKFDDL
                     FKKFELEAASGGGPDLFIAPNDSLGKEARAGLFLDITTMLDGKLGNDSDVSVAGSKVD
                     GKLYMVPESLKAVAMYYDKSKIATPPATTDALLAGVKDGSIKAGFDAHSSYHSFGWWA
                     GFGGKLMDDSGKCVADTTGVADAYKYFQDLQTAGAKWYEKYDDLASDFKSGKIDLIVD
                     GPWASGGYKDALKDNLAVAPMPAGPKGPSAPLTGVDGWYINTNAKDSALAVNFALEMV
                     KTENEQVFVDTAGHIPADKTIKITDPITQKFAEAVATGFARPQVQQLDNFWGNFDNAL
                     NLVIQKHEDPTKAATDACKAMNTANKIP"
     gene            complement(2531..3562)
                     /locus_tag="VHM48_07275"
     CDS             complement(2531..3562)
                     /locus_tag="VHM48_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2755248.1"
                     /translation="MTIANVAERAGVSKTAVSFAFNNPERLNVATASRILDIANELGY
                     RPDPVARMLTQRRTDSIGILTPQDLSIIFTNPFFGAFSAGIAAVAEERGYSLHFISPL
                     QGSLSRAIHRAIVDGVVAIGLTDDHPEIDEIRRAGLPMVLVDSATSGDSASIDVDDEG
                     GARLAATHVIELGHRDVAILAIDGPRSTAGGGHGVVERRMRGYRETLAGAGIDLPTDA
                     IAVGAASIDGGIEAFDRIWDSGRRPTAVLAMSDAMAIGAIRAIHQRGSSVPGEISVVG
                     FDDVDLAASTDPPLTTVHQPVQGKGEAAVRLLLSVMEGGHSAPVHRRLETRLVIRSST
                     GPVSQAGRR"
     gene            3815..5998
                     /locus_tag="VHM48_07280"
     CDS             3815..5998
                     /locus_tag="VHM48_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX2755249.1"
                     /translation="MTRRISLALTLHNHQPVGNFGWVIAETFERAYRPMVDALERHPG
                     IRVALHYTGPLLAWLRAERPEFIARLAMLAERGQIEIVGGGWYEPVLAALPERDRVGQ
                     LVRMADELETTFGRRPRGAWLAERVWEPDLPTALVSAGYDWTVLDDAHFRAAAIPEDD
                     LWGSYTTDDQGKVLTVFGTEQGLRYRIPFAEVDDVIGYLRDHATEDGERLGTMGDDGE
                     KFGAWPTTWDHCWGPGRWVERFFEALDANADWLTTIPPSDWTGGHRPVGRVYLPTGSY
                     AEMGEWALPADEGLAFGSALRRARAEHRPEARWLRGAIWRNFQVRYREINDIHKQMLA
                     ASDLVEAMPAGPVRTTATDHLLAGQSNDCYWHGLFGGIYLPDLRVAALARLIAAEDLA
                     VGHGAASGVRRDVDLDGRDEVVLAGPGQFVTVKLEEGGGIPRWDLRAAGHPLGAVMRR
                     RPEAYHETLRDHESAGDHEPAEQTEGGAPASIHEIVMVKQEGLLAHLVYDAYERRSGL
                     IRILPTDTTPDQAAAGAAAELGDLRDGTWSLERLDAEEVFARRTGAIRTGNGSAAVPV
                     EATRTIAIGGGRLDPWLELSLEIVHRGTTDDPPIDALVAVEWSTMLLGGGHNPSAWLE
                     AGGERTAHDATRSASAICRIAAGNDFLGIAVETTTDHPVDAWIAPIETVSNSEAGFEL
                     VYQGSSTLLVEPLRLRSGERWSLRITQQASVTAERFPLAGAANTE"
     gene            5995..7398
                     /locus_tag="VHM48_07285"
     CDS             5995..7398
                     /locus_tag="VHM48_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX2755250.1"
                     /translation="MSRGRLAVHAHFYQPLRLDPWTCRVPTEPSAAPFHDWNQRVAAE
                     CYRPNAERGNLRRISWNLGPTLASWMDAEDPATYRRFIADSAPAIAQSYHHAILPLAS
                     AADRRTEIAWGIRDFELRFGRRPAGLWLPETAVDLATLREAVRQGIDYTILAPWQAAE
                     PAIDTRRPYRVDLGGDEGIVAFFYDAGLSASVSFEPEATFDADRFARERVVPRLRGGP
                     APVDAPLAVLATDGELYGHHQQFRDLFLQRLLVPDPNAADRGFDIVTLDALVAEASND
                     GLAAIHIVDRTSWSCQHGVARWSGECPDAADGRWKGALRAAFERLAGAIDVVTAAAFA
                     ELPGDVDADAARDAYVEVVSGHVPPATFTADRWPGADDAARGRALDLLEAQRWRLAMF
                     ASDGWYWDDPIRSETRQVLRAAARAVRLVDTTAGTRLERRLVDDLATFVSPSRGLDGA
                     GIYRMALADVDQPPPAA"
     gene            complement(7448..8035)
                     /locus_tag="VHM48_07290"
     CDS             complement(7448..8035)
                     /locus_tag="VHM48_07290"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2755251.1"
                     /translation="MCASCFDFIIRHHGGAEIECSLLFADIRGSTAMAERMSPAQFRE
                     RLDRFYATATAVVFDHDGGVDKFIGDELMALFYPLLSGDGHVARAIEAARALLLATGH
                     AEPLGPWLPVGAGVHTGPAWVGAVGDGSHTELTAVGDTVNVTARLAAAAGAGEILVTV
                     AAAQAAGLDPRLERRWLQLKGKRAPTEVVSLSAGS"
     gene            7997..>8372
                     /locus_tag="VHM48_07295"
     CDS             7997..>8372
                     /locus_tag="VHM48_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755252.1"
                     /translation="MMADDEVEAAGAHRRILVRLSLPEHPHHGRPDAGKGSRAELAPR
                     AFRDPLEEAPQLSFGGIATRQKVAPERFIVTCGLSRRWHLNGHGMPLTQARSAGIDAI
                     STANVDPGAVARLAGPVVQPGIQ"
BASE COUNT         1253 a         2897 c         2941 g         1281 t
ORIGIN      
        1 tagctcgcgg tcgcccgtgc caaccggttg gtgaccaggg tgatcccgag aagcaggaag
       61 aagaggatca ccgagaaggc cgccgcgacg ccgtacagct ggaagtcgtt gacgagcttg
      121 tacgcctcgg tcacgaacag ctccgtccga ccgaacgggc cgccgttgga catgaagtac
      181 gacaggtaga acaggttgaa cgtcacgacg aagccgtaga tcgcgtacgg cagcatcgcc
      241 ggccggagga acggcaacgt cacgaccttg aagcgctgcc aggcgttcgc gccgtccatc
      301 tcggccgcct cgtagagatc cggcgggacg ctctgcaggg cacccgtggc gaccaccgag
      361 ttgaggggcc agccgagcca cgtgttcgcc gtgagcaggg cgaaataagc gagcggcagc
      421 gggaggaacg ggatcgggtc gtcgacctgg ccgagccagt cgatccggaa ggcgtccggc
      481 gggatcttga acagcgagcc gatcccctgg agaaccatgt tgatcgcgcc gttgtcctgg
      541 tcgaacagat gtcgccagac cgtcgcgacg atgatcggcg ggatgatgac ggggaggatg
      601 aagagggccc gatagacctt cttgaagcgc agcccgttgg tgttcagcag gatcgcgatc
      661 cccacgccga ggatgacgtg gatgacgacg ttggagaagg cccagaagag gttgaagaag
      721 agcagccgca ggaactcgaa gttcgggatg ttcagcttgc tctggacgat ctggacgtag
      781 ttgtccgctc cgacgaggtt cggggcgacc ttgccgctga ggttcttcag cccgaagtcg
      841 gtgaacgaca tccaggtctg gaagagcaac gggtagaacg tgatcacgcc catcaccagg
      901 aacgccggca acaggtagaa cagggccgtc cgcgtgtccg gagagaagcg ccggcggcgg
      961 cggggttgcg ccaacgtgat cgctggggcg gtcatgaccg tgtcctcgtg accagggctc
     1021 ggaaaggccg ggggtgcctg ccgtacggat cgtacgacct ggcgggtctg ctgggttggc
     1081 cgcccgggcg ccggcggagc cgacgcccgg gcaggcgttc agatcacggg atcttgttgg
     1141 ccgtgttcat cgccttgcag gcgtcggtcg cggccttggt cgggtcctcg tgcttctgga
     1201 tgacgaggtt cagggcgttg tcgaagttgc cccagaaatt gtcgagctgc tggacctgcg
     1261 gacgggcgaa cccggtcgcg accgcttcgg cgaacttctg ggtgatcgga tcggtgatct
     1321 tgatcgtctt gtcggccggg atatgacccg cggtgtcgac gaagacctgc tcgttctcgg
     1381 tcttgaccat ctcgagggcg aagttgaccg cgagggccga gtccttggcg ttggtgttga
     1441 tgtaccagcc gtcgacgccg gtcagcggag ccgatgggcc cttcggaccg gccggcatcg
     1501 gagcgaccgc gaggttgtcc ttgagggcgt ccttgtagcc gcccgaggcc cacggcccgt
     1561 cgacgatgag gtcgatcttg ccggacttga agtcgctggc gaggtcgtcg tacttctcgt
     1621 accacttggc gccggcggtc tgcagatcct ggaagtactt gtaggcatcg gcgacgccgg
     1681 tcgtgtcggc gacgcacttg ccgctgtcat ccatcagctt gccgccgaac ccagcccacc
     1741 agccgaagga atggtacgag ctgtgggcgt cgaagccggc cttgatcgaa ccgtccttga
     1801 caccggccag gagcgcgtcc gtcgtcgccg gcggggtcgc gatcttggac ttgtcgtagt
     1861 acatggcgac ggccttgagg gactcgggaa ccatgtagag cttgccgtcg accttggagc
     1921 ccgcgaccga cacgtcggag tcgttgccga gcttgccgtc gagcatggtg gtgatgtcga
     1981 ggaacaggcc ggcgcgggct tccttgccga ggctgtcgtt cggggcgatg aagaggtccg
     2041 gcccgccgcc gctcgcggcc tcgagctcga acttcttgaa caggtcgtcg aacttgatgt
     2101 cctggatcgt cagcttgaga tccgggttgg cggccgagat cttgtcggtc agggtcttga
     2161 aggcggtcgg ctcggtgccg gcgccggagc cgtacgaagc ccagaccgtc agcgcgccct
     2221 tgagaccggt ggccggggcg gtgctgccgc ccgcggtgct gggagcgggc tggctggcac
     2281 cagtgccggt cgagccggcc ggcgtcggcg tcgcgccgct cgaacaggcg ccgaccacga
     2341 tggccaaagc gccgagggtg ctgagcaccc gaagcttggt attcatctcg agtgaatctc
     2401 ctcctggttg atggatggcc ggccggaccg gcccgttgcc gccgtcgtga gcggcgggac
     2461 ctcgttcgat cgcgcggcga ccgctggtcg ggtccgcgac tcgttcaggc tgacttcggt
     2521 ctgcccgccg tcacctcctg cctgcctgcg acacgggtcc ggtcgaagac cggatcacga
     2581 ggcgcgtctc gagccggcga tgcacgggcg cggaatgccc gccctccatc accgacaaca
     2641 gcagccggac ggccgcctca cccttgccct ggacgggctg gtgaacggtc gtcaggggtg
     2701 gatcggtcga cgcggcaagg tcgacgtcgt cgaacccgac gacgctgatc tcgccgggga
     2761 ccgagcttcc ccgctggtgg atcgcgcgga tggcgccgat cgccatcgcg tcgctcatgg
     2821 ccaggacagc cgtcggccgt cggccgctgt cccagattcg atcgaacgcc tcgatcccgc
     2881 cgtcgatgct ggcggcgccg acggcgatcg catccgtcgg caggtcgatc ccggcaccgg
     2941 ccagggtctc gcgatagccg cgcatgcggc gctcgacgac gccgtgtccg ccgcccgcgg
     3001 tcgagcgcgg cccgtcgatg gcgaggatcg ccacgtcgcg gtggccgagc tcgatgacgt
     3061 gcgtggccgc gaggcgcgcg ccaccctcgt cgtcgacgtc gatcgaggcg gagtcaccgc
     3121 tggtcgccga gtcgacgagg accatcggca gaccggcccg ccggatctcg tcgatctcgg
     3181 ggtggtcgtc ggtcagcccg atcgcgacga cgccgtcgac gatcgcccgg tggatcgccc
     3241 gcgacagcga gccctgcagg ggcgagatga agtggaggct gtagccgcgc tcctcggcga
     3301 ccgccgcgat gccggcgctg aaggcgccga agaacgggtt ggtgaagatg atcgaaagat
     3361 cctggggggt caggatgccg atcgagtcgg ttcgccgctg ggtcagcatc cgggcgaccg
     3421 ggtcgggccg atagccgagc tcgttggcga tgtcgaggat ccgcgacgcc gtcgcgacgt
     3481 tgagccgttc cgggttgttg aacgcgaacg agacagccgt cttcgagacg cctgcccgct
     3541 ccgccacgtt cgcgatcgtc acccgtcggg cgacctgatc caaaaccgct cctcctcggg
     3601 ccgcagcccg ccgccgcctc ttcgatcctt cgtcccgccc gacccgtcgg tcgcgggttg
     3661 cggggtgtgt atcattgggc ggcaaaaccg gtttagcaaa ccggtttacg gaccgtagca
     3721 gagacgccct cgcgacgtca atgggcgtca ttcgcaacaa tctgcaacga ctgccgccgc
     3781 gacccccgcc tcgccgattc caagaggaga ccgcgtgacc cgccggatct cgctggccct
     3841 gaccctccac aaccatcaac cggtcggcaa cttcggctgg gtgatcgccg agaccttcga
     3901 gcgcgcctat cgcccgatgg tcgacgccct cgaacgtcac cccggcatcc gggtcgcgct
     3961 ccattacacg gggccgctcc tcgcctggct gcgagccgaa cgccccgagt tcatcgcccg
     4021 tctcgcgatg ctcgccgagc gcggccagat cgagatcgtc ggcggcggct ggtacgagcc
     4081 cgttctcgcc gccctgcccg aacgcgatcg cgtcggccag ctcgtccgca tggccgatga
     4141 gctcgagacg accttcggcc gccggccgcg cggggcgtgg ctggcggagc gggtctggga
     4201 accggatctc ccgacggccc tcgtctcggc cggctacgac tggaccgtcc tcgacgacgc
     4261 ccacttccgg gcagccgcca tccccgagga cgatctctgg ggctcgtaca ccaccgatga
     4321 ccagggcaag gtcctgaccg tcttcggcac cgagcagggt ctccgctacc ggatcccctt
     4381 cgccgaggtc gacgacgtca tcggctacct ccgcgatcac gccaccgagg acggcgagcg
     4441 tctcgggacg atgggtgacg acggcgagaa gttcggcgca tggccgacga cgtgggacca
     4501 ctgctgggga cccggccgct gggtcgaacg gttcttcgag gcgctcgacg ccaacgccga
     4561 ttggctgacg acgataccgc cgtccgactg gaccggcggc catcgaccgg tcgggcgggt
     4621 ctacctcccg accggctcgt acgccgagat gggcgaatgg gccctgccgg cggacgaggg
     4681 actcgcgttc gggtcggccc tccgccgcgc ccgggcggag caccgccccg aggcccgctg
     4741 gctgcgcggc gcgatctggc gcaacttcca ggtccgctac cgtgagatca acgacatcca
     4801 caagcagatg ctcgcggcaa gcgacctggt cgaggcgatg ccggccggac cggtccggac
     4861 gaccgccacg gaccatctcc tggccggcca gtccaacgac tgctactggc acggcctgtt
     4921 cggcgggatc tacctgcccg acctccgggt cgcggcgctc gcccggctga tcgccgccga
     4981 ggatctcgcg gtcggtcacg gagcggcctc cggcgtgcgc cgggacgtcg acctcgacgg
     5041 ccgcgacgag gtcgtcctcg cggggcctgg ccagttcgtg acggtcaagc tcgaggaggg
     5101 cggcgggatc ccgcgctggg acctccgggc ggccggtcat cctctcgggg cggtgatgcg
     5161 ccgccggccg gaggcctatc acgagacgct tcgcgaccac gagtcggccg gtgatcacga
     5221 accggcggag cagacggagg gcggcgctcc cgcctcgatc catgagatcg tcatggtcaa
     5281 gcaggagggc ctcctcgccc acctcgtgta cgacgcctac gaacgacggt ccggcctgat
     5341 ccggatcctg cccaccgaca cgacgcccga ccaggccgcc gccggggcgg ccgccgagct
     5401 cggcgacctg cgggatggca cgtggtcgct cgagcgcctc gacgccgagg aggtgttcgc
     5461 ccgccggacc ggagcgatcc ggaccgggaa cgggtcggcg gccgtgccgg tcgaagccac
     5521 ccggacgatc gccatcggcg gcggacggct cgatccgtgg ctcgagctgt cgctcgagat
     5581 cgtccaccgc gggacgaccg acgatccgcc gattgacgcc ctcgtcgcgg tcgagtggtc
     5641 gacgatgctc ctcggcggcg gccacaaccc gtcggcctgg ctggaagccg ggggcgagcg
     5701 gacggcacac gatgcgactc gctcggccag cgcgatctgc cggatcgcgg ccggcaacga
     5761 tttccttggc atcgccgtcg agacgaccac ggaccacccg gtcgatgcct ggatcgcccc
     5821 gatcgagacc gtgtccaact ccgaggcggg gttcgagctc gtctaccagg gcagctcgac
     5881 cctgctcgtc gagccgttgc ggctccggtc gggcgagcgc tggtcgctgc ggatcaccca
     5941 gcaggcctcg gtcaccgccg aacgcttccc gctggccgga gcggcgaaca ccgagtgagt
     6001 cgcggtcggc tcgcggtcca tgcccacttc taccagccct tgcggctgga cccgtggacc
     6061 tgtcgcgtcc cgaccgagcc gtcggcggcg ccgttccacg actggaacca gcgagtcgcc
     6121 gccgagtgct accggcccaa tgccgaacgc ggcaacctcc gccgcatctc gtggaacctc
     6181 ggaccgacgc tcgcatcctg gatggacgcc gaggaccccg cgacctaccg gcgcttcatc
     6241 gccgacagcg ccccggcgat cgcccagtcc taccaccatg cgatcctgcc gctcgcctcg
     6301 gccgcggatc ggcgcaccga gatcgcctgg gggatccgcg acttcgagct ccgattcggt
     6361 cgccgcccgg ccgggctctg gctgcccgag accgccgtcg acctggcgac cctgcgcgag
     6421 gccgtccgcc agggcatcga ctacacgatc ctggccccgt ggcaggcggc cgaaccggcg
     6481 atcgacacgc gtcgccccta ccgggtcgac ctcggcggag acgaggggat cgtcgccttc
     6541 ttctacgacg ccgggctgtc ggcctcggtc tcgttcgagc cggaggccac gttcgacgcc
     6601 gaccgcttcg cccgcgagcg cgtcgtgccc cgcctgcgcg gcggaccggc gcccgtcgac
     6661 gcgccgctcg ctgtgctcgc gaccgacggc gagctgtacg gccatcacca gcagttccgg
     6721 gacctgttcc tgcagcggct gctcgtcccg gacccgaacg cggccgatcg cgggttcgac
     6781 atcgtgaccc tcgacgcgct tgtggccgag gcttcgaacg atggtcttgc ggcgatccac
     6841 atcgtcgacc gcacttcgtg gagctgccag cacggcgtcg ctcgctggag cggcgaatgc
     6901 ccggacgcgg ccgacggtcg ctggaagggc gcgctgcggg ccgccttcga acggctggcc
     6961 ggagcgatcg acgtcgtcac cgcggccgca ttcgccgagc tgcccggcga cgtcgacgcc
     7021 gacgccgcgc gggacgcata tgtcgaggtc gtgagcggtc acgtcccgcc ggcgacattc
     7081 acggccgatc gctggccggg cgccgacgac gcggcgcgcg gacgtgcgct cgacctgctc
     7141 gaggcccagc gctggcgtct cgcgatgttc gccagcgacg gctggtactg ggacgacccg
     7201 atccgatccg agacgcgcca ggtgctccgg gcggctgccc gcgctgtccg actcgtcgac
     7261 acgacggccg ggacgcggct ggagcggcgc ctcgttgacg acctcgcgac gttcgtgtct
     7321 ccgtcgcgcg gcctcgatgg ggccgggatc taccggatgg ccctcgccga cgtcgatcag
     7381 ccgccgcccg ccgcttgacc cggccccgct ccgggctcgg accagcggct ggtcgggacg
     7441 gacgccctca ggatccggcc gagaggctga cgacctccgt gggcgcccgc ttgcccttga
     7501 gttggagcca ccgacgctcg aggcgagggt cgagaccagc cgcctgcgcg gccgccaccg
     7561 tgaccaggat ttcgccggcg cccgccgccg ccgcgagccg ggccgtcacg ttgacggtgt
     7621 ctccgaccgc cgtgagctca gtgtgggatc cgtcgccgac cgcgccgacc caggccgggc
     7681 cggtgtggac cccggccccg accggcagcc agggtcccag gggttcggcg tggccggtgg
     7741 cgaggagcaa cgcccgggcc gcctcgatcg cccgcgcgac gtggccatct ccgctgagca
     7801 gcgggtagaa gagggccatc agctcgtcgc cgatgaactt gtcgacaccc ccgtcgtgat
     7861 cgaacacgac cgccgtcgcc gtggcgtaga agcgatccag gcgttcgcgg aactgggccg
     7921 gtgacatccg ctcggccatc gcggtcgacc cgcgaatgtc ggcgaacaag aggctgcact
     7981 cgatctcggc gccgccatga tggcggatga tgaagtcgaa gcagctggcg cacatcgtcg
     8041 gattctggtc cgactgtcgc ttcccgagca tccgcatcac gggcgccccg acgccggcaa
     8101 agggagccgc gcagagcttg caccgcgggc cttccgggat ccgcttgaag aggcgccgca
     8161 gctttcgttc ggtggcatcg ccacgcgtca gaaagtcgcg ccagaacgct tcatcgtgac
     8221 gtgcgggctc tcgagacgct ggcatctcaa cggacacggt atgcccctca cgcaagctcg
     8281 atctgccgga atcgatgcga tttcgacagc aaacgttgat ccgggcgcgg tggcgcggct
     8341 ggccgggccg gtcgtgcagc cgggaatcca ac
//
LOCUS       DATAIW010000231         4776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_213929, whole genome shotgun sequence.
ACCESSION   DATAIW010000231 DATAIW010000000
VERSION     DATAIW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213929"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VHM48_07300"
     CDS             complement(<1..762)
                     /locus_tag="VHM48_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755253.1"
                     /translation="MPARKTRAKVASRAGAKPRAEEKAAADAAAAAALSQAAAGAADV
                     TRGEDEATIAATYSALSAAAARRGGRDAAEGAATLDMADEVAAGGALAAALSTDEFRR
                     GMDLAGIAGQVQVAADLVRGVGQSTLAAFLGATSQQLRVLATEALGRATEGALVAHGA
                     DHLAGQLAALGRTEMGEGRDEYASSDALGAASAEMAAAAVRSAAAGAAELAAATAMGG
                     MAQALEHDGPKPPSGAGAVDQGSPGKAAATRRASKP"
     gene            1054..1407
                     /locus_tag="VHM48_07305"
     CDS             1054..1407
                     /locus_tag="VHM48_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX2755254.1"
                     /translation="MKVKLVTFGEIQIEGDRYTSDVVIDGGVTRRRRKGPSKALRHLF
                     GHTPLSIAEDVPWGGKRLIIGTGADGALPVAPEVYQEGARRRIAIEAVPTRDACRLLA
                     GLKPEDVYAVLHVTC"
     gene            complement(1743..1816)
                     /locus_tag="VHM48_07310"
     tRNA            complement(1743..1816)
                     /locus_tag="VHM48_07310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1780..1782),aa:Glu,seq:ttc)
     gene            complement(1891..2277)
                     /locus_tag="VHM48_07315"
     CDS             complement(1891..2277)
                     /locus_tag="VHM48_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755255.1"
                     /translation="MSDRRRGPDRAGTASRERPAESWRLGPIPITVTGVLIVVALILS
                     LGYVGYAITVRDPSQIPLLASGLVVLGLVFVAIAAVGARATWRSSVRGSDARAFGHAI
                     VGGFAALVAAGCIAGAVILILVNNGF"
     gene            2332..3396
                     /locus_tag="VHM48_07320"
     CDS             2332..3396
                     /locus_tag="VHM48_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2755256.1"
                     /translation="MRIAQLAPTYERVPPRTYGGTEIIVHLVTEELVARGHDVTLFAS
                     GDSLTSAVLRSVTPVPMRYGETSAEGLGHAEYLQLANAQAAFRAAADGAFDLVHNHAG
                     IEGLVLAADSRTPVISTMHNPFAPSTRPIWDAYPWFHHAVSAASAATFPTRGALPPIL
                     HGIDVRSFVFEPSPAAGYLLFLGRFSKAKGADRAVEVARRTGRRLILAGKIDAADAEH
                     VRATIAPLFDGEQIEYVGEVDRTVKRDLLAGADALLFPIEWDEPFGLVMIEALSSGTP
                     VIGFRRASVPEVIEDGRTGFVVDDVDGMVEAIARIGEIDRRVCRSVAEERFSVARMVD
                     DVEAMYRSVLDRVGAGAAGA"
     gene            complement(3408..4016)
                     /locus_tag="VHM48_07325"
     CDS             complement(3408..4016)
                     /locus_tag="VHM48_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755257.1"
                     /translation="MSDLPFSLPPKTAAVLASALDRERKIERTLEALGPIAGRDAVLV
                     GGGPDETARWAAAGARLTSVETLLDDRGQALPDGSADAIVSAWSGFRGVDPAELAAAD
                     RVLRPTGHLLVIHDYGRDDVSRLRGDLSEYGTWSRRNGPFLSNGFRVRVVHCFWTFDT
                     LDQAREFLGAAFGPEGSAFGEALQRPRLSYNVAVYHRTRGGT"
     gene            4091..4462
                     /locus_tag="VHM48_07330"
     CDS             4091..4462
                     /locus_tag="VHM48_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755258.1"
                     /translation="MVRQFLAMPGGLRLFLVYALTILFGIGISLRYVIDQAIGAPVSG
                     PGVVVMVLLAYTIFTTTLVIQRKQAARGLALGLASLTLPLTVYLLAAGLIPQTLFFAA
                     LAALLFRGLLRDEVRAWLNEE"
BASE COUNT          678 a         1706 c         1686 g          706 t
ORIGIN      
        1 cggcttcgac gcacgccgag tcgcggccgc cttgccagga gagccttggt cgaccgcgcc
       61 ggcgccggat gggggcttgg gcccgtcgtg ctccagcgcc tgggccatgc cgcccatcgc
      121 cgtcgcggcg gcgagttctg cggctcctgc ggccgccgat cggacggctg ccgcggccat
      181 ttcggcactc gcggcgccaa gcgcatcgga ggacgcgtac tcgtcgcgcc cctcacccat
      241 ctcggtccgt ccgagcgccg cgagctgacc ggcgaggtga tccgctccgt gggccaccag
      301 agcgccctcg gtggcccggc cgagcgcctc ggttgccagg acgcgcagct gctggctcgt
      361 cgctccgaga aacgccgcga gcgtcgactg gccgacaccc cggacgagat ccgccgccac
      421 ctggacctgg ccggcgatgc ccgcaagatc catcccgcgg cgaaactcgt cggtgctgag
      481 cgccgcggcc agtgctccgc cggcggcgac ctcgtcggcc atgtcgaggg tcgcagcgcc
      541 ctcggccgcg tcgcgcccac ctcgccgggc ggccgcggcg ctcagggccg aataggtggc
      601 tgcgatggtc gcctcgtctt cgccgcgggt cacgtcggcg gctcctgccg cggcttgact
      661 cagcgcggcc gcggccgccg cgtcggcggc tgccttctcc tcggcgcgag gcttggctcc
      721 ggcccgggac gcgaccttgg ccctagtctt ccgagcaggc atctcgtcct cctggaggtc
      781 ggccgccaca ttcggccgtc atcaggaccg atcccttggc gacttcatgt gcccggcaga
      841 ggcccccggt tggcgcgatc tgaccaaacc ccaaaaggga tcgtcgtcga cgtgcggagc
      901 gggggccgca cctatcctag cgctccggcc aaatctcacg tccgttgact ggatgcgcga
      961 ggcgcgatct gctcatcgtg ctgccggtcg tcgccgcggc gcggacgggg gcgcgtcggt
     1021 tcccatttga ttcgggggtg ggagaatgtc gacgtgaagg tgaagctggt tacgtttggc
     1081 gaaatccaga tcgagggcga ccggtacacg tccgacgtcg ttatcgacgg tggagtcact
     1141 cgccggcgtc gcaagggccc ctccaaggcg ctccgacacc tgttcgggca caccccgctg
     1201 tcgatcgccg aggacgttcc ctggggcggg aagcggctca tcattggaac gggggctgac
     1261 ggcgcgcttc cggtcgcacc cgaggtctac caggaaggcg cgcggcgacg gatcgcgatc
     1321 gaagcagtcc cgacccgcga tgcctgccgc ctccttgcgg ggctgaagcc ggaggacgtc
     1381 tacgcggtcc tccacgttac ctgctgagcg gccacttcgc tgcacgatgc ttccatcgtg
     1441 ttcctactcg ggccactcca cgatggctca ggccgcgatc gccgtggccc tgacccgcag
     1501 cagcgtggcg ataagctcgt ccggaccgcc tcgcggctct gcccgacgac gaccggatcc
     1561 gctcgtttga tgcctaccgt gcgcgcgtcc tcgggttgcc cgatgcgatc gccgtggcct
     1621 cgcccgctcg tcgggaggag ctgtgccgga tcgtgatcga acggatcgtc gtcagagacc
     1681 ggcaggtcga ggaaatcgcc tggacgccac ccgctcgacc gttcttcgaa agacagcggg
     1741 tgtgccccca aggggattcg aacccctgat ctccaccttg aaagggtggc gtcctaggcc
     1801 tctagacgat gagggcgagg ccgggccgag tgtatcaggg gacccctgtg cgccggccga
     1861 tctcgtccgg atcgcgcggc ggcggcgcga tcagaagccg ttgttgacca ggatcaggat
     1921 gaccgcgccg gcgatgcacc cggcggcaac cagggcggcg aacccgccga cgatcgcgtg
     1981 gccgaatgcc cgggcgtcgc tgccgcgaac gctcgagcgc cacgtcgcac gggcgccgac
     2041 agcggcgatc gcgacgaaga cgaggccgag gacgacgagg ccggaggcca gcagcgggat
     2101 ctgcgaggga tcgcgaacgg tgatcgcgta gccgacatac ccgagcgaca ggatcagcgc
     2161 gaccacgatg aggacaccag tcaccgtgat ggggatcggg ccgagccgcc agctctccgc
     2221 cgggcgctcg cggctggcgg ttcccgctcg gtccgggcca cgtcgtcgat cgctcatcag
     2281 cggggagggt agcagggcgg atacgatcga cgcgtcacag ggggatatcg aatgcggatc
     2341 gcgcagcttg cgccgaccta tgagcgggtg ccgccgcgga cctacggcgg gaccgagatc
     2401 atcgtccatc tcgtgaccga ggagctcgtc gcccgcggtc acgatgtcac gctgttcgcc
     2461 agcggcgact cgctgacgtc ggccgtcctg cgcagcgtca ccccggttcc gatgcgctac
     2521 ggcgagacat ccgccgaggg cctcggccac gcggagtacc tccagctcgc caacgcccag
     2581 gcggcgttcc gcgcggccgc cgacggcgcg ttcgacctcg tccacaacca cgccgggatc
     2641 gagggcctgg tcctggcggc cgactcgcgg accccggtca tctcgacgat gcacaacccg
     2701 ttcgcgccat cgacccgccc gatctgggac gcctacccgt ggttccatca cgccgtctcg
     2761 gcggcgagtg cggcgacgtt cccgacgcgc ggcgccctgc caccgatcct ccatgggatc
     2821 gacgtccggt cctttgtgtt cgagccgagt cctgcggccg gctacctgct gttcctcggc
     2881 cgattcagca aggcgaaggg cgccgaccgg gcggtcgagg tggcccgccg caccggccgg
     2941 cgtctgatcc tggccggcaa gatcgacgcg gccgacgccg agcacgtccg ggcgacgatc
     3001 gcgccgctgt tcgatggcga gcagatcgag tacgtcggcg aggtcgatcg aaccgtcaag
     3061 cgcgacctgc tcgccggtgc cgatgccctc ctgttcccca tcgaatggga cgagccgttc
     3121 gggctggtga tgatcgaggc gctcagctcc gggacaccgg tcatcggctt ccgccgggcg
     3181 agcgtgcccg aggtgatcga ggacggccgg accgggttcg tggtcgacga tgtcgacggc
     3241 atggtcgagg cgatcgcacg gatcggcgag atcgaccggc gcgtctgccg gtcggtggcg
     3301 gaggaacgct tcagcgtcgc ccgcatggtc gacgacgtcg aggcgatgta tcgctccgtg
     3361 ctcgatcggg tcggtgcggg tgcggcgggc gcctgagcgg gatctggcta cgtgccgccg
     3421 cgtgtccggt gatagaccgc aacgttgtac gagagtcgag gccgctgcag cgcttctccg
     3481 aacgccgaac cctcggggcc gaaggccgcg ccgaggaact ccctggcctg gtcgagcgtg
     3541 tcgaatgtcc agaagcagtg gacgacccgg acccggaagc cgttcgacag gaacggcccg
     3601 ttgcgccggc tccaggtgcc gtactcggag aggtcgccgc gcaaccgcga gacgtcgtcg
     3661 cggccgtagt cgtggatgac gaggaggtgg ccggtcggtc gaaggacgcg gtccgccgcg
     3721 gccagctcgg cgggatcgac gccgcggaac cccgaccagg cgctgacgat cgcgtcggcc
     3781 gagccatcgg gcagggcctg cccgcgatcg tccagcagcg tctccacgct cgtcaagcgg
     3841 gcgcccgcgg cggcccagcg ggccgtctcg tcgggcccgc cgccgaccag cacggcatcg
     3901 cgaccggcga tcgggccgag cgcttcgagg gtgcgctcga tcttgcgctc ccgatcgaga
     3961 gcggaagcga gcacagccgc ggttttcgga ggcaggctga acgggaggtc cgacacggag
     4021 cggaaggata acccgagccg gcctgcgagc ccgacgtgcg ttcccctccg tgtcatgtca
     4081 cgatccggac gtggtccggc aattcctggc gatgcctgga gggctccgcc tcttcctcgt
     4141 ctatgccctc acgatcctgt tcgggatcgg gatctcgctc cgctacgtca tcgaccaggc
     4201 gatcggcgcg ccggtcagtg gtcccggggt cgtcgtcatg gtcctgctcg cctacacgat
     4261 cttcacgacc accctggtca tccagcgcaa gcaggcggcg cgcgggctcg ccctcgggct
     4321 ggccagcctc acactccctc tgaccgtgta cctgctcgcc gccgggctga tcccccagac
     4381 gctcttcttc gcggcgcttg cggcgctcct cttccgcggg ctgctccgcg acgaagtaag
     4441 ggcctggctc aacgaggagt gacggcacgc accgccagtc ggccgcgggc cggcctccgg
     4501 actgacgcca cgggccggtt gacggtaccc ttgccggact tgcgcggggc ccatggtcct
     4561 gcgcgcacgc gcgcgaacgg gcggcgcgtg ccttcccgac ccgaactgag acgaggcacg
     4621 accgaaccgt gagcaaggaa tcacgccacg cggccaggct ggcgcgcgag agccgtcgcg
     4681 ccggaaccac ggcccgccac tcggaccccg gcgccggacc gaccgacgcc gccgagggcg
     4741 gagtcgtcgg cggctccccg acgcggccga ccggcc
//
LOCUS       DATAIW010000232         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_12413, whole genome shotgun sequence.
ACCESSION   DATAIW010000232 DATAIW010000000
VERSION     DATAIW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12413"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="VHM48_07335"
     CDS             <1..1751
                     /locus_tag="VHM48_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2755259.1"
                     /translation="RQVLTARENARLLTDRARSAARFQAIIQNANDVIAVVDPEGIIT
                     YVTPSVMRLLGRPAEGLPGQGLDTLLEPQDVPLALALLRTARTRPGTSDTIQCQVRDV
                     QENARHIEMNVTNLLGESVVEGLVVTMRDVTERRRFEEQLRDQALHDPLTGLANRVLI
                     ADRIDHALRRRRRLAGATPTLLYLDLDDFKEVNDSLGHPVGDQVLVEVARRLSRAIRA
                     EDTPARLGGDEFAVLIDESRSVEEVVAVADRILADLRTPIDVAGTTVAIRASIGIVRR
                     DAGPEPEDFLRDADIAMYEAKREARGGHRLFESAMFAATVERASQETDLRAALAAGQF
                     EVVYQPLFDLSDNQLAGVEALLRWNHPTRGLVMPLQFIPLAERTGEIIPIGRWVIEQA
                     CLTVGGWSGINEARQLRASVNVSARQLEPRLVEDVAEILRRTGFPAKRLVLEIAESVI
                     AAERPGVMDVLAALRSLGVRISIDDFGTGYAPLSVLRHLPVDELKIDRSFINALSDQG
                     DTSLVKAIIKLSHDLALATVAVGIEGEEQLALLRTLGYDIGQGFLLARPGPASAIEDR
                     IRRELDPSSGARVPGA"
     gene            complement(2025..2735)
                     /locus_tag="VHM48_07340"
     CDS             complement(2025..2735)
                     /locus_tag="VHM48_07340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2755260.1"
                     /translation="MTAIDPKAVLGDVSGGRVLDVATGTGSFVRFLLDGLRDHAEVVG
                     IDVNPERGQAFAAAIGDRPDVRFEQMDAHHLVFPDGSFDTVCVSNSLHHFADPAPVLA
                     EMLRVLRPGGHLVVNEMCRDGQSETQTTHVLLHHWWAAVNRLRGEVHRETYERGRIVE
                     IVEGLGLSDLRIVDLADPDEDPHDPETTAELEAAIDRYAVLAEGHPELQARGEALRTR
                     LREVGVRGATQIVATGRR"
BASE COUNT          400 a         1061 c          991 g          428 t
ORIGIN      
        1 tgcgccaggt gctgaccgcc cgggagaacg cccgcctcct cacggaccgt gcccggagcg
       61 ccgcccggtt ccaggcgatc atccagaacg cgaacgacgt gattgcggtg gtcgatccgg
      121 aggggatcat cacctacgtc acgccgtcgg tgatgcgtct tcttggccgg cccgccgagg
      181 gcctccccgg ccagggactc gacaccctgc tcgagcccca ggacgtccca ctcgcgctcg
      241 cgctcctccg gacggcgagg acccggcccg gcacgagcga cacgatccag tgccaggttc
      301 gggacgtgca ggagaatgcg cgtcacatcg agatgaacgt gacgaacctc ctcggtgaat
      361 cggtcgtcga gggcctcgtc gtgacgatgc gcgacgttac cgagcgccgc cggttcgagg
      421 agcagctccg cgatcaggcg ctccacgatc ccctgacggg cctcgcgaac cgcgtcctca
      481 tcgcggaccg gatcgaccac gcgctccggc gccgtcggcg tctcgccggc gccaccccca
      541 cgctgctcta cctcgacctt gacgatttca aggaggtcaa cgattcgctt ggccacccgg
      601 tcggcgacca ggtcctcgtc gaggtcgccc ggcgcctcag ccgcgccatc cgggccgagg
      661 acacgcccgc ccgcctgggt ggtgacgaat tcgcggtcct gatcgacgag tcgcgctccg
      721 tcgaggaggt agttgccgtc gccgatcgga tcctggccga cctgcgcacc ccgatcgacg
      781 tcgccgggac gacggtcgcc atcagagcca gcatcgggat cgtccggcgg gatgctggcc
      841 ccgaaccgga ggacttcctc cgcgacgccg atatcgcgat gtacgaggca aaacgcgagg
      901 cgcgcggcgg ccaccggctc ttcgagagtg cgatgttcgc cgcgacggtc gaacgcgcaa
      961 gccaggagac ggatctgcgg gccgccctgg ctgcgggcca gttcgaggtg gtctaccagc
     1021 ccctcttcga cctgtccgac aatcagcttg ccggcgtcga ggcgctcctc cgctggaacc
     1081 acccgacgcg ggggctggtg atgccgctgc agttcatccc gcttgcggag cgcactggcg
     1141 agatcatccc gatcggccgc tgggtcatcg agcaggcgtg cctgacggtc ggcggatgga
     1201 gcgggatcaa tgaggcccgc cagctccgcg cgagcgtgaa cgtatcggcc cggcaactcg
     1261 agccgcggct cgtcgaagac gtcgccgaga tcctgcggcg gaccgggttc cccgccaagc
     1321 gcctcgtcct ggagatcgcc gagagcgtca tcgcggcgga gcgcccgggc gtgatggacg
     1381 tcctggccgc gctgcgatct ctgggcgtgc gcatctcgat cgacgatttc ggtaccggtt
     1441 atgcgccgct cagcgtcctg cgccacctgc ccgtcgacga gctcaagatc gaccgctcct
     1501 tcatcaatgc cctcagcgac cagggcgata cgagcctcgt caaggcgatc atcaagctca
     1561 gccacgacct cgccctggcg accgtggccg taggcatcga aggcgaggag caactcgcgt
     1621 tgctccgcac gcttggctat gacattggcc agggcttcct ccttgcgcgc ccgggcccgg
     1681 cgagcgcgat cgaggaccgc atccgccgcg agctcgatcc ctcgtccggc gcccgcgtcc
     1741 ccggcgcctg atcggcgccc actccgctcg tgatcggccg tcggctcggc cccccgcctt
     1801 gttccagcgg cgagccgctc caacgggcac cgtgttcgtt gcgtgtcctc ggccgtcccg
     1861 cgcgctgacc ggccgacgcg ggccgctcgg cactggcgcg ggcgcacggc gggcgctcgg
     1921 gatcacccca cggccggtgt cggagtacct gcggaccctg gccgggccgg ccgccgcctg
     1981 acccgctggc gccgaccctg cggcgggcgc ccgacgacgg ccggtcagcg ccgtccggtc
     2041 gccacgatct gggtcgcgcc ccggacgccg acctcccgga gccgcgtccg cagggcctcg
     2101 ccgcgcgcct ggagctccgg gtgtccttcg gcgagcacag cgtaccggtc aatcgccgcc
     2161 tccaactcgg ccgtggtctc cggatcgtgg gggtcttcgt ctgggtcggc caggtcgacg
     2221 atccgcaggt cggagagccc gaggccttcg acgatctcga cgatccgtcc ccgttcgtag
     2281 gtctcccggt gcacctcgcc gcggagccgg ttcacggccg cccaccagtg gtgcaggagg
     2341 acgtgcgtgg tctgggtctc ggactggccg tcgcggcaca tctcgttgac gacgaggtgg
     2401 ccgccggggc gaaggacgcg aagcatctcg gccagcacgg gggcagggtc cgcgaagtgg
     2461 tggagggagt tcgagacgca gacggtgtcg aacgagccat ccgggaagac gaggtggtgg
     2521 gcatccatct gctcgaaccg cacgtcgggg cggtccccga tcgccgccgc gaacgcctgg
     2581 ccccgctccg ggttcacgtc gatcccgacg acctccgcgt gatcccggag gccgtcgagc
     2641 aggaagcgga cgaagcttcc tgtcccggtg gcgacgtcga gcacgcgccc gccggacacg
     2701 tcgccaagga cggccttcgg gtcgatcgcc gtcatcgtcg ctccgccgcc aggcctggcg
     2761 cctggtccgg gagcgcgatc gggcgcccgg tcaggcgccc gactccctac caccgctggt
     2821 ggacgagcgg cttcgcgcgc ttctcgtaga gctcgcggat ccggtccatc gtcgccggcg
//
LOCUS       DATAIW010000233         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_42945, whole genome shotgun sequence.
ACCESSION   DATAIW010000233 DATAIW010000000
VERSION     DATAIW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42945"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VHM48_07345"
     CDS             <1..185
                     /locus_tag="VHM48_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755261.1"
                     /translation="PIPADDPLLSLDNCLVVPHIASASRATRSKMAEMTAANLLAGVR
                     GDRIPTPVNPEVYDRA"
     gene            complement(448..870)
                     /locus_tag="VHM48_07350"
     CDS             complement(448..870)
                     /locus_tag="VHM48_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2755262.1"
                     /translation="MAAPSNQIRIGRAAEMLGVTVDTVRRWADEGRLTVVRSEGGQRL
                     VPIEEVTRLTGARRQAAIDRPIVAQSARNRFAGIVTRIEKDRVAAVVEVQAGPHRLVS
                     LMTAEAVIEMGLKVGDEAICVVKSTNVVVEIPSPREPR"
     gene            930..1004
                     /locus_tag="VHM48_07355"
     tRNA            930..1004
                     /locus_tag="VHM48_07355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:962..964,aa:Lys,seq:ttt)
     gene            complement(1106..1708)
                     /locus_tag="VHM48_07360"
     CDS             complement(1106..1708)
                     /locus_tag="VHM48_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755263.1"
                     /translation="MEGNHMATENPGDLQQPAPDVPPPPVAPAPASPAPAPASPAPAP
                     AGPAFNLTAADFKFTRASIVMVAAGIITVVGYFLPWASVSGKVLGQNLGSSVAGSDFL
                     VALLGIAMIAGGAVFAYHPPQFKIAWIAVGIIAIIALVVMLRYFGDYNNAKSALGGLG
                     GLAGISVDLGLGFLIVVVGAIVAIVGAALGRGETLGRRMF"
     gene            complement(1960..2289)
                     /locus_tag="VHM48_07365"
     CDS             complement(1960..2289)
                     /locus_tag="VHM48_07365"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2755264.1"
                     /translation="MSVPLPVVCEPRFDLRAPDVTGAVAAAALLSDRTRASILRMLAD
                     GPTCVCEIATALGERDNNVSNHLARLRDAGLVRASRHEANARFLYYERDDEAVARARA
                     ALDHVLR"
BASE COUNT          374 a          859 c          877 g          338 t
ORIGIN      
        1 agccgatccc ggccgacgat ccgctgctgt ccctcgacaa ctgcctcgtc gtgccgcaca
       61 tcgcgtccgc ctcgcgggcg actcggtcga agatggccga gatgaccgcg gcgaacctcc
      121 tggcgggcgt ccgcggcgac cgcatcccga ccccggtcaa cccggaggtc tacgaccggg
      181 cgtgattgcg ggggcgcgtc ggcccactgc ggctcgcgct ggctcgcgct ggctcgcgcg
      241 ggctcgcgcg gatgcctcgc ccggcgcggc ggcgcactga gcccgcgcgc gactcgtcgc
      301 ccagcgcgcc cgaatcgaga tcgttcaccg caagcagaac aacgccgtcg gtctgcggcg
      361 ctgatcgaca ttggccgaga tcattccgcg tggcgtgaac gaaggcgaaa tgcggtatct
      421 ggggtgacca acccgtgggc ggccgaccta ccgcggctcc cgcggcgacg ggatctcgac
      481 gacgacgttc gtcgacttca ccacgcagat cgcctcgtcg ccgaccttga gtcccatctc
      541 gatgacggcc tcggccgtca tcaggctgac gaggcgatgc ggcccggcct ggacctcgac
      601 gaccgcggcg acccgatcct tctcgatccg ggtcacgatc ccagcgaacc ggttgcgggc
      661 ggactgggcg acgatcgggc gatcgatcgc ggcctggcgt cgtgcgccgg tcaggcgggt
      721 gacctcctcg attggaacga gccgctgacc gccctcggag cggacgacgg tcaagcgccc
      781 ctcgtcggcc cagcgccgga cggtgtcgac ggtcacgccg agcatctccg ccgcgcggcc
      841 gatccggatc tggttcgacg gggcagccat gtcgagcgat tgtatgggtg gcagctcggt
      901 cgccgtctcg tcggctacgc ttcgcgctcg ggccggtagc tcagtggcag agcaggggac
      961 ttttaatccc ttggtcgtgg gttcgaatcc cacccggctc gccacgcatc cctcgcacgc
     1021 ccggtcctgg ggaagccgcg ccgtgacgca acggcccgcc ggtcgccgcc gtccggcggg
     1081 cgcgttcgcc gctgttgaag gagggctaga acatcctgcg gccgagcgtc tccccacggc
     1141 cgagcgccgc gccgacgatc gcgacgatgg ctccgaccac gacgatcagg aagccgagcc
     1201 cgaggtcgac cgagatcccc gcgaggccgc ccagtccgcc gagcgccgac ttggcgttgt
     1261 tgtagtcgcc gaagtacctg agcatgacca cgagggcgat gatcgcgatg atcccgaccg
     1321 cgatccacgc gatcttgaac tgcggcgggt ggtaggcgaa caccgcgccg ccggcgatca
     1381 tggcgatgcc aagcagagcg acaaggaagt ccgacccggc caccgagctg ccgaggttct
     1441 gcccgaggac cttgccgctc acgctggccc atggcaggaa gtagccgacg accgtgatga
     1501 tgccggcggc gaccatcacg atgctcgccc gggtgaactt gaaatcggcg gcagtcaggt
     1561 tgaacgccgg gccggcgggt gccggggccg gcgacgccgg agccggggcc ggcgacgccg
     1621 gagccggcgc gaccgggggt ggtggaacat ccggcgccgg ctgctgcaag tcgcccggat
     1681 tctcggtcgc catgtggttc ccttccatcg ttccctcctg gatgtgacga gtatcaccgc
     1741 cgcgtcaaca gcggcgtgac gccagaccgc atcttgagcg gtcatccagg gctcgaagac
     1801 ggcgatggcc gtgtcgttgt gccggatccc gtcagcgccc cgaggatgcc cgctgcgggc
     1861 gactcggcct ggagcgctgc gacgaggaat gcgaaggtaa cgcaggaacg aggcgaggca
     1921 gcccgaacgg cggatggccg cgctgagcgg tgagggtggt catcggagga cgtgatcgag
     1981 cgcggcgcgg gccctcgcga ccgcctcgtc gtcccgctcg tagtagagga atcgggcgtt
     2041 ggcctcgtgg cggctggcgc ggaccaggcc cgcatcgcgc aacctggcca ggtggttgct
     2101 gacgttgttg tcccgttctc cgagggcggt cgcgatctcg cacacgcagg tcggcccatc
     2161 ggccagcatc cgcagaatcg aggcgcgggt ccggtccgac agaagcgcgg ccgcagccac
     2221 ggcgcccgtg acatcggggg cgcgaaggtc gaagcgcggc tcgcagacga ccggcagcgg
     2281 aacgctcacc gcctggtctc cggcaggggc atgccgccgg cgacgatcgg cagggcccgg
     2341 acgccgaaca ggcgcggcgc caggcgcatc gccgcccaga cgaggaacag catcaccggg
     2401 acctcgatga gcgggcccac caccgtcgcc agcgccaccg tcggcgag
//
LOCUS       DATAIW010000234        13040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_12874, whole genome shotgun sequence.
ACCESSION   DATAIW010000234 DATAIW010000000
VERSION     DATAIW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13040
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12874"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHM48_07370"
     CDS             complement(<1..332)
                     /locus_tag="VHM48_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755265.1"
                     /translation="MGAMSRIARLALAGALVGGALGMPGPTRSPVMAVAVTSTAPTIA
                     QLIGQKLVVRMDGLSPSPALLGRIQRGEVGGVILFGANITTAGALRQLTATLRAAAAA
                     GGRPRLFIA"
     gene            complement(320..997)
                     /locus_tag="VHM48_07375"
     CDS             complement(320..997)
                     /locus_tag="VHM48_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEX2755266.1"
                     /translation="MPRDRASRVLDEFSAVTSAAPRPDSPAERIVMRSRFPVATLSMA
                     SLVVVAVAVAAIVLGRSGPSPAAGSSPAPSTVPSAPVSVASAESTIGPCAPTELSARV
                     TMWEGAAGQRIADVELTNRGTTDCLLDALARPQLVGGDGGVLIDGKNPSTSGQLTVAP
                     GAVLKSLVAAGNYCKSAPIPPVSVAFVFADGRRLVADPVSPADVTLPPCNGAGVPATI
                     SMHAWAP"
     gene            complement(997..1473)
                     /locus_tag="VHM48_07380"
     CDS             complement(997..1473)
                     /locus_tag="VHM48_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2755267.1"
                     /translation="MAIAEPTDLAFERAFAEHWTDVFRFALAWTNDWTAAEDLAQDAY
                     LRLWNQRDRLDWSRPVMPWLLVATRRLATDRFRILRRRLLAPTHAGALDTDPALRARW
                     LDVCVAMSDLSSLERTALVMTAVQGATYDEAGRVLGTTPGALRAAVSRARAKLENA"
     gene            1556..2299
                     /locus_tag="VHM48_07385"
     CDS             1556..2299
                     /locus_tag="VHM48_07385"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX2755268.1"
                     /translation="MTTPSTGPRILGIVGGTGPESTIDYYRSLIAVWRRRRPDGSYPR
                     VIINSVESGRVIRLLADGEYDAAGRELGAALGQLAAAGCGRALLASNLSHMAFDRLDP
                     PPPIPLIHIVDAARDAAVAAGHRRLGLIGTRFVMQAHLYQDRFGTDGIAIVVPLPDEQ
                     EMVHAIYFGELVHGEIRDDSRERLVAFIAAMRDRDGIDGLILGGTELALTLKAPLYAG
                     VPILDTAHIHVEAAVDWLLADDGVAADGG"
     gene            2366..2776
                     /locus_tag="VHM48_07390"
     CDS             2366..2776
                     /locus_tag="VHM48_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2755269.1"
                     /translation="MADTREMAAKFEEVLKAGDFARMGELIQEYATDDFVEEWPQSGE
                     RLTRAAALRMGETYAQKTGTSPSFTYKRMLGGGDVFIIEGTIDYGDGVPVSYVGVGEL
                     RDGKVARMTEYFANPFEAPAWRADFVERMEPVKA"
     gene            complement(2766..4046)
                     /locus_tag="VHM48_07395"
     CDS             complement(2766..4046)
                     /locus_tag="VHM48_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755270.1"
                     /translation="MPGTRRRRSARSRCSCSNRSRSRRTEPGEHGESRPDLTPARSRT
                     AAVLGCLSFFLIGWSGLLVASLIRSIEHDFGQTDAGMGFFYLVNAAVYGTGVLGGTLV
                     TQRFGRRPVLAGGAGLVALGLVVLATAETWSIFLVAVIPFGIGCGTLDGAGNGLILDL
                     FPDTRGRALNLLHFCFSAGALASPAVVGRAVAAGVPWRSLLLATAIVAVPVAVLFVRA
                     AMPTGRHVRGIDGARPGRRLLLDRVLLVLGLAIACYVAAEVGVSNWLVRFLAAAPLDV
                     ATTSLSLYWAGLTVSRIAAARIGDRFDHTTFAAVASIATGALILLAIVAPSLELSIVL
                     FAAAGVASGPIFPLIVAIGGDRFPHRSAAVGGYLAGAAVVGGISYPPVMGFLSVTIGL
                     PAAMAGTGALAILAAVVLAGARRPRSVGADPVRP"
     gene            complement(3961..4419)
                     /locus_tag="VHM48_07400"
     CDS             complement(3961..4419)
                     /locus_tag="VHM48_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2755271.1"
                     /translation="MPLNGDYAPGTSAWARKQSEIYEATGGVQGAALHGKPVVVLTSI
                     GAKTGNLRKTALMRVEHGGVYAVVASLGGAPSHPVWYHNLKADPRVELQDGPTKRDYT
                     AREVSGDEKARWWARAVEAWPDYARYQEKTERQIPVFVLEPIQEPEDGTR"
     gene            4673..4855
                     /locus_tag="VHM48_07405"
     CDS             4673..4855
                     /locus_tag="VHM48_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755272.1"
                     /translation="MALLHPNLLERVSAHRDGLRVLVMLVAVLAAMAVLTVAFGVTHT
                     GPSYELVPDPAAGLPF"
     gene            5011..5142
                     /locus_tag="VHM48_07410"
     CDS             5011..5142
                     /locus_tag="VHM48_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755273.1"
                     /translation="MAPSCENRRDPASSRRIKAMDKKAKTPKKPKQTKTKDGAAKGK"
     gene            complement(5218..6408)
                     /locus_tag="VHM48_07415"
     CDS             complement(5218..6408)
                     /locus_tag="VHM48_07415"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEX2755274.1"
                     /translation="MTGRIAVLALSVSLVIGACIPPTSQPPSSPVESSAGPAASPVVG
                     SSQPPRSPAPTPPTVLALSVGQRPSGPWAVTFQRNGSEAIREVYVLAPACEQASCDID
                     ATIQTFAGDPIGTGVFRFVDGMYRYEANHQDTVACDDGLGLVPDGATAISHTTLVIAG
                     YRPVGTAVVSVDIRGTRTVEVAPRGGSACAAASLDYTANGAKTEFAAAPTPTPKPPAA
                     PKVPVIGDAFFGSNATVVTYGVAGSTIPQIIDSIQASGPWSAWIHARAEGLTKAVPKY
                     RFVLAQTAGQCRINVTAKPAILFTYTITLPRWSRPKTVDAATVRWWAGELQRVATHER
                     HHVEIYRDGATRMTDAVARSNCANLSGRLAAIVKDIDTQQCEFDLKEYGSALGLSLSS
                     CLKQ"
     gene            complement(6515..7057)
                     /locus_tag="VHM48_07420"
     CDS             complement(6515..7057)
                     /locus_tag="VHM48_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX2755275.1"
                     /translation="MRALVVYESMFGNTKAVALAIADGIRPTKEVDSFEVSEVHATLP
                     FDVDLLVVGGPTHAHGLTSSKTRADASERAGIRLVSRGSGIREWLDALLPGGTEVAAT
                     AFDTRIKGPELLWGSAAKGAAKRLKTLGFRVLTPTSFVIGGPAGDPFDRVSDDEIRRA
                     RNWGHTLAMAVDGAPVGTSR"
     gene            complement(7384..8103)
                     /locus_tag="VHM48_07425"
     CDS             complement(7384..8103)
                     /locus_tag="VHM48_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2755276.1"
                     /translation="MTIANATATDPRGRYIETTPAPDLGAIKQRQQATWSSGDDHMIG
                     TQITIVSELLIEALDLHSTERVLDVATSSGNAALAAARRGCDVVGLDYVPSLLDRARR
                     RADADGLEAEFVAGDAEDLPFADASFDVVSSVFGAMFAPDQEQTAREFARVCRPGGRI
                     GLVAHTPEGFIGQLFKVNAGHVPPPTGLRSTVLWARKSAWASSSAMRSRRSAWRGGNT
                     CSVTARPRPTSTTGDGSTGRR"
     gene            complement(8270..9019)
                     /locus_tag="VHM48_07430"
     CDS             complement(8270..9019)
                     /locus_tag="VHM48_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX2755277.1"
                     /translation="MEATDWQTIVRQNHPRYRSARRRSHWAMLFIGLATAAIALEILV
                     VARGFSFVEHFGSVTLAEITTWERNLNTVSGLYIASIVPAAIAFLAWQSRVVDNIPAL
                     SSTDPWVTPRWSIALWFVPLASFVAPYRIVADAWRRLAGRPAQRNAGLVLIWWILWLG
                     GGLTNRIVQAFTQPPETAPALSSLLTWITGAAVAQVVAGLLLVWIIVEIERRSADRAA
                     ANARSDLESPPADLAMETPAIEPRAIEPSAD"
     gene            9114..10052
                     /locus_tag="VHM48_07435"
     CDS             9114..10052
                     /locus_tag="VHM48_07435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2755278.1"
                     /translation="MSVWFGYHMPSFTFPGVPASGLFDHLATLASAAEDAGFSLVTVM
                     DHFYQIIGVGPETDPMLEAYTALAALSQRTRRVRLGALVSGVTYRNPALLAKTVTTLD
                     VLSGGRALLGIGAAWNESEHEGYGFDFPRVGERMDRLDEALTIIRAMFTEERPTFDGE
                     HYRIHDALNVPRPIQSGGPRILVGGGGEQRTLRIAAKHADLTHWFPLGLETLRHKNEV
                     LEGYCAEIGRDPATIERTMATPVLVAPNEAAAKAMLERIPPERRGHVEIGPPEQAAEG
                     LRPYIEAGFTGFTFNNPIVSTPESIHAVGELLRLIA"
     gene            complement(10120..10899)
                     /locus_tag="VHM48_07440"
     CDS             complement(10120..10899)
                     /locus_tag="VHM48_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX2755279.1"
                     /translation="MDAITLTVRLPGSRARWIAAGLATGLLVATIASPSLGPRSTLAA
                     DPTSPPQHTISVSGVGTVVVAPDVADLRLGVTFNRPTVKAARADAAAAMTKVIAALKA
                     LGIADKDIRTSTISLQPTYDYTTNTNPPRITGYSFSNGVAVTVRDLDKVGDAIDDSLA
                     AGATTLDGVSFRVDDPAKAEQQARTDAMSQARAHADTLAAAAGVAITGVVSISETSSP
                     TPYPIFYGAATAGAAARDVATPVQAGTTDVTITVGVVYLIG"
     gene            complement(10994..11584)
                     /locus_tag="VHM48_07445"
     CDS             complement(10994..11584)
                     /locus_tag="VHM48_07445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755280.1"
                     /translation="MTIELPARLQMTIAALDAEAYVAAIPGLALLVIDAVAGGASVNF
                     MAGVTEQEATAWWTGRIPEIADGSTTAFVARLPDETGEGGPGAVVGSTLLIRSRNANS
                     PHRAEVGKVLVQRTARRRGLGRALMLATEERARADGRWLLILDTEAGSAADAFYRSLG
                     WHELGTMPNHAYRSDGVLAPTTYFWKDLRATARNGP"
     gene            complement(11574..12530)
                     /locus_tag="VHM48_07450"
     CDS             complement(11574..12530)
                     /locus_tag="VHM48_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2755281.1"
                     /translation="MTRRGLVLFGLMSVIWGIPYLFIRVAVAEISPAALVLARTSIAA
                     AILLPYAIARVDLRRVLARWRWVVAFAAIEIALPWVALGSAEQHVSSSLAGLLIAGVP
                     LVGTTFALATGGTDRLGRTGLVGLGIGLVGVAAIVWGDFATSDPSALLEIGVVVVCYA
                     VGPAILARRLGGLPSVGVMAISLALCAAAYVPITAFQWPSIVPSVNVVGSIAILAVVC
                     TAAAFLVFAALIAEIGPVRATVITYVNPAVAAVLGVLVLHEKLTPAMAIGFGLVIAGS
                     TLATRPSRAAVDARPARDTDRVALRRARRAALSCRRRCGRDD"
     gene            12640..>13040
                     /locus_tag="VHM48_07455"
     CDS             12640..>13040
                     /locus_tag="VHM48_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX2755282.1"
                     /translation="MAHDLERFLVAQEYLYDGVLDELRRGQKTGHWIWFIFPQIAGLG
                     RSALSQQFAISSLGEARAYVAHPVLGVRLRECAAALLATTPRTAEQILGSIDAMKVRS
                     CMTLFHRAAPDEPVFSQVLDRYYAGAADEATV"
BASE COUNT         2073 a         4448 c         4653 g         1866 t
ORIGIN      
        1 gcgatgaaga gacgtggccg gccacctgct gcggccgccg cccggagggt cgcggtcaac
       61 tgccgcaggg cgccggcggt cgtgatgttg gcgccgaaga ggatgacgcc gccgacctcg
      121 ccgcgctgga tccggccgag cagcgcgggg ctcgggctta ggccgtccat tcgcacgacg
      181 agcttctggc cgatcagctg ggcgatggtc ggggccgtgg aggtcacggc caccgccatc
      241 acgggggatc gcgtcggtcc gggcatcccg agcgccccgc cgacgagtgc gccggcgagc
      301 gcgaggcggg cgatccggct catggcgccc acgcatgcat cgagatcgtg gcgggtacac
      361 cggcgccgtt gcacggtggg agcgtcacgt cggccgggct gaccgggtcc gcgacgagtc
      421 gccggccatc ggcgaacacg aaggcaacgc tgaccggcgg gatcggcgca gacttgcagt
      481 agttgccggc cgcgacgagc gacttcagga ccgccccggg cgcaaccgtc agctggccgc
      541 tcgtcgatgg gttcttgccg tcgatcaaca cgcctccgtc gccgccgacc agctgcggcc
      601 gggccagggc gtcgagcagg cagtccgttg ttcctcgatt ggtcaactcc acgtcagcga
      661 tccgctgacc ggccgctcct tcccacatgg tgacgcgggc cgacagctcg gttggagcgc
      721 acggaccgat ggtcgattcg gcagaggcga ccgagacagg cgcgctgggc accgtggatg
      781 gcgcgggcga cgagccggca gccggactcg gcccggagcg gccgagcacg atggctgcga
      841 ccgccaccgc gacgaccacc aggctggcca tggacaaggt cgcaacggga aatcggctgc
      901 gcatgacgat cctctcggcg ggggagtcgg gtcggggcgc cgcgctcgtc acggcgctga
      961 actcatcgag cacgcggctc gctcggtccc tcggcatcag gcgttctcca acttcgctcg
     1021 ggctcggctg accgcggcgc gaagcgcccc gggtgtcgtc ccgagcactc ggccggcttc
     1081 gtcgtaggtc gcgccctgga ccgcggtcat gacgagggcg gttcgttcga gcgatgacag
     1141 gtcgctcatg gcgacgcaca catcgagcca gcgggcacgg agggcaggat ccgtgtccag
     1201 cgcgccggca tgggtcggcg cgagcagccg tcggcgaagg atccggaacc ggtccgtggc
     1261 caggcgtcga gtggcgacca ggagccacgg catgacgggc cgactccagt cgaggcgatc
     1321 gcgctggttc cacagccgga gatatgcgtc ctgggccagg tcctccgccg cggtccagtc
     1381 gttggtccag gcgagggcga accggaacac gtcggtccaa tgctccgcga acgcacgctc
     1441 gaaggccaga tcggtcggtt cggcgatggc cacgatggtt gtcacaccca gatacacgac
     1501 ggcgcggtgc caaacgcacg gacgccccag actggcacag cgtaggatcc gcgcgatgac
     1561 gacgccatcg accgggccgc gcatcctcgg gatcgtcggg gggaccgggc ccgagtcgac
     1621 catcgactac taccgctcgc tgatcgcggt ctggcgccgc cgccggcccg acggcagcta
     1681 tccgcgcgtg atcatcaaca gcgtcgagtc cgggcgggtg atccgcctcc tcgctgacgg
     1741 ggagtacgac gcggcagggc gcgagctggg agcggcgctc ggccagctcg cggcggccgg
     1801 ctgcggtcgg gcgctcctcg cctcgaatct ctcgcacatg gcgttcgacc ggctcgatcc
     1861 acctccgccg attccgctga tccacatcgt cgatgccgcc cgcgacgcag ccgtcgccgc
     1921 cggccatcgc cgtctcggac tcatcgggac gcgcttcgtg atgcaggcgc acctctacca
     1981 ggatcgattc ggaacggacg gcatcgcgat cgtcgtcccg ctgccggacg agcaggagat
     2041 ggtccacgca atctatttcg gcgagctcgt ccacggcgag attcgcgatg acagccgcga
     2101 gcggctcgtc gccttcatcg cggcgatgcg cgaccgggac gggatcgacg gcctgatcct
     2161 cggcggaacc gagctggccc tgacgttgaa ggcgcccctg tacgccggtg ttccgatcct
     2221 cgacacggcc cacatccatg tcgaggccgc cgtcgactgg ctgctcgccg acgatggagt
     2281 cgccgcagat ggtggataac tcgcttgtcg cgcggcgtca tgaggtgcaa tctgggccgt
     2341 ccacgacacg cacgagggac attcgatggc tgacaccagg gagatggcgg cgaagttcga
     2401 ggaggtcctc aaggccggtg acttcgcgcg gatgggcgag ctgatccagg agtacgccac
     2461 cgacgacttc gtcgaggaat ggccccagtc gggcgagcgc ctgacccggg cggcagcgct
     2521 gcggatgggt gagacgtacg cgcagaagac gggaacgagt ccgagcttca cctacaagcg
     2581 gatgctcggc ggcggcgacg tcttcatcat cgagggcacg atcgactacg gcgacggcgt
     2641 cccggtcagc tacgtcgggg tcggcgagct ccgggacggc aaggtcgcgc ggatgaccga
     2701 gtacttcgcc aacccattcg aggcgccggc ctggcgcgcc gacttcgtcg agcggatgga
     2761 gcccgtcaag gcctgacggg atcggctccg accgagcggg gacggcgcgc tccggcgagg
     2821 accacggccg cgaggatcgc cagcgcccct gtcccggcca tcgcggccgg cagcccgatc
     2881 gtcaccgaca ggaaccccat gaccggggga tagctgattc cgccgacgac cgcggctcct
     2941 gcgaggtagc cgccgacggc agccgaacga tgagggaatc gatcgccgcc gatcgcgacg
     3001 atgagcggga agatcgggcc ggaggccacg ccggccgccg cgaacaggac gatcgatagc
     3061 tcgagcgacg gcgccacgat cgcgaggagg atcagggcgc ccgtcgcgat gctggcgacg
     3121 gccgcgaagg tggtgtggtc gaagcggtcg ccgatccggg cggccgcgat ccggctgacg
     3181 gtcaggcccg cccagtagag gctgagcgag gtcgtggcaa cgtccagcgg ggccgccgca
     3241 aggaagcgca cgagccagtt cgatacgccg acctccgcgg cgacgtagca ggcgatggcc
     3301 aggccgagca cgaggaggac gcggtcgagg agcaggcgac ggccgggccg ggcaccgtcg
     3361 atcccgcgga cgtgtcgtcc ggtcggcatc gcggcccgca cgaacagcac ggcgaccggg
     3421 acggcgacga tggccgttgc gagcagcagg gagcgccacg gaacgcccgc cgcgacggca
     3481 cgcccgacga ccgccggcga ggcgagggcg cccgcgctga agcagaaatg gaggaggttc
     3541 agcgcccgac cgcgggtgtc ggggaacagg tcgaggatca gcccgttccc cgctccgtcc
     3601 agcgtcccgc agccgatccc gaacgggatg acggcgacga ggaagatcga ccaggtctcg
     3661 gccgtggcga gcacgaccag tccaagcgcc accagccccg ctccgccggc gagcaccggt
     3721 cggcgaccga accgctgcgt gaccaaggtg ccgccgagga cgccggtccc gtacacggcc
     3781 gcgttgacga ggtagaagaa ccccatgccg gcgtccgtct ggccgaagtc gtgctcgatc
     3841 gatcggatca gggacgccac gaggagtccc gaccaaccga tgaggaagaa cgagaggcag
     3901 ccgaggaccg ctgcggtcct cgacctggcc ggcgtcaggt ccggccgact ttcgccatgc
     3961 tcaccgggtt ccgtcctccg gctcctggat cggttcgagc acgaacaccg ggatctggcg
     4021 ctccgtcttc tcctggtacc tggcatagtc gggccaggcc tcgactgcgc gcgcccacca
     4081 gcgtgccttc tcgtcgccgc tgacctcgcg tgccgtgtag tcgcgcttgg tcggtccatc
     4141 ctggagctcg acgcgggggt cggccttgag gttgtgatac cagacgggat ggctcggtgc
     4201 gcccccgagg gacgcgacga ccgcgtacac gcccccgtgc tcgacgcgca tcagggcggt
     4261 cttgcgcagg ttgccggtct tcgccccgat cgaggtcagc accaccacgg gcttgccatg
     4321 caatgcagcg ccctgcacgc cgcccgtcgc ctcgtagatc tcgctctgct tgcgagccca
     4381 ggcggaggtg ccgggtgcgt aatcgccgtt cagtggcatg gaccgattct tccacggatc
     4441 ggcgcgtccc gtgaccgccc atgggctgtt cgaaattcgg gccgggcttg cctggctgca
     4501 cgggtggcgg agcggtcaaa tcacgtgcgc tccggcactt gaacggtgcg gtatcgatag
     4561 ccagattagc cgggtagaga tcagcgccag cggcgtgccg acgcgcgagt cgatcgggtt
     4621 gccgatccgc gcgggctgac ggatcccgat ttgtcgaccc gacggaggcc ccatggctct
     4681 tctacacccg aacctcctgg aacgcgtctc ggcgcaccgg gacgggctcc gcgtgctggt
     4741 catgctcgtc gcggtgcttg cggctatggc cgtcctgacc gtggccttcg gcgtgaccca
     4801 caccgggccg agctacgaac tggtgcccga tccggcggcc ggcctgccgt tctgacttgc
     4861 gccttgaagc atcccctcgc ctgatcgttc gatcgcgcct gaccgcggtc gatgaccggg
     4921 tcacgtcggc gtgcgctcgc tcgcgcgatc ccgcgccagg ttgacttggc tcgacccgaa
     4981 tacgtcccat gtcatgttgt ccgtgccgcg atggctccgt cgtgcgagaa tcggcgggac
     5041 ccggcgtcat ctcggaggat caaggcgatg gacaagaagg cgaagacccc caagaagccc
     5101 aagcagacca agacgaagga cggcgcggcg aagggcaagt agcggcgaca actggcgccc
     5161 gcaacagcac ggccaggtgg cgtccgcacg tggcgcggac atccagccgc caggcggtca
     5221 ctgcttgagg caggacgaga ggctcaggcc gagagcgctc ccgtactcct tcaggtcgaa
     5281 ctcgcattgc tgcgtgtcga tgtccttgac gatcgccgcg agccgacccg acaggttcgc
     5341 gcagttgctg cgggcgacgg cgtccgtcat cctggtggcg ccgtcacggt agatctcgac
     5401 atggtggcgc tcatgcgttg caacccgctg caactccccg gcccaccagc ggaccgtcgc
     5461 ggcgtcgacc gtcttcggcc ggctccacct cggcagcgtg atcgtgtacg tgaacaggat
     5521 ggccggcttc gcggtgacgt tgatgcgaca ttggccggcg gtctgcgcga gcacgaagcg
     5581 gtatttcggc accgccttgg tcaggccttc cgcacgggcg tggatccatg cgctccaggg
     5641 cccgcttgcc tggatcgagt cgatgatctg cgggatcgtg ctccccgcca caccgtacgt
     5701 gacgacggtg gcgttcgacc cgaagaatgc gtcgccgatc accggcacct tgggcgcagc
     5761 aggcggtttc ggagtcggcg tgggcgccgc cgcgaactcg gtcttggcgc cgttcgcggt
     5821 gtagtccaga gacgcggcgg cacaggcgct tccgcccctc ggggcgacct cgacggtgcg
     5881 ggtgccgcgg atgtcgaccg agacgacggc cgttcccacc ggtcgatagc cggcgatcac
     5941 gagggtcgtg tggctgatgg cggtcgcgcc gtccgggacg aggccgagcc cgtcgtcgca
     6001 ggcgaccgtg tcctggtgat tggcttcgta gcggtacatg ccgtcgacga accggaagac
     6061 gccggttccg atcggatcgc cggcgaacgt ctggatcgtg gcgtcgatgt cgcacgacgc
     6121 ctgctcgcag gccggcgcga ggacgtagac ctcgcggatg gcctcgctgc cgttccgttg
     6181 gaacgtgacc gcccacgggc ctgagggacg ttgcccgacg ctgagggcga gaaccgtcgg
     6241 cggagtcggg gccggcgatc gcggtggctg gctcgagccg acgaccggtg acgccgcagg
     6301 acctgcagag gattcaacag gggacgacgg tggctgggac gtcggcggga tgcacgcccc
     6361 gatcacgagg ctgacggaga gcgcgagaac tgcgattcga cccgtcatga gccccacccc
     6421 gacctgccag cgacgatcgg tcgatcctac ccgacgatct tccgcccgtc gcccctgacg
     6481 aaatgcggca gtaggggtcg gggtcctcct ggcctcagcg cgacgtgccg accggcgctc
     6541 cgtccacggc cattgcgagg gtgtgtcccc aattgcgggc acgccggatc tcgtcgtcgg
     6601 acactcgatc gaacggatca ccggccggtc cgccgatgac gaagcttgtc ggcgtcagga
     6661 cccggaaccc gagcgtcttg agacgcttcg ccgcgccttt cgcggcagag ccccagagca
     6721 gctccgggcc cttgatccgc gtgtcgaagg ccgtggccgc gacctcggtc ccaccgggca
     6781 gcagggcatc gagccactcc cggatcccgg agccgcggga gacgagccgg atcccggcac
     6841 gttcggaggc gtcggcgcgc gtcttcgagc tcgtcaggcc atgggcgtgg gtcggtccgc
     6901 cgacgacgag caggtcgaca tcgaacggga gcgtcgcgtg gacctccgac acctcgaagg
     6961 agtcgacttc tttcgttggg cggatcccgt cggcgatcgc gagggccacc gccttcgtgt
     7021 tgccgaacat cgattcgtag accaccaggg cgcgcatctc cggatcctcc acacgaccgc
     7081 cacgaggcaa ccgaggaccc ggccggcacg gaacggatcg cctcgctgcg acaggccaag
     7141 ggtggccagc gacggcgaca cgaccaagag ccatgcggcc cccgcaaatg ggacacttgg
     7201 ccgcgtcccg accgctcggg ccgggacgcg atcctgcgac ctggcgtcgg acgatgacgg
     7261 tctcgaggta ctccgtcggc acgaccatgg tcccgtcgtc ggctcgattg aaccgcgcga
     7321 tgagatcgag caggtcggcc tcgagggctc ggtgtccggc ttcgccgacc gcctcgaacg
     7381 ccttcagcgt cggcccgtag aaccgtcgcc agtagtcgag gtgggcctcg ggcgagcggt
     7441 aacggaacac gtattgccgc ctcgccacgc ggatctccgc gatcgcatcg ccgaagagct
     7501 cgcccaggcg ctcttccgtg cccacagaac cgttgagcgc aggcccgtcg gaggcgggac
     7561 gtgaccggcg ttcaccttga agagctggcc gatgaagccc tcgggggtgt gggcgacgag
     7621 cccgatccgg ccgcccggcc ggcagacgcg ggcgaactcg cgggccgtct gctcctggtc
     7681 cggtgcgaac atcgccccga acaccgagct gacgacatcg aagctggcat ccgcgaacgg
     7741 caggtcctcg gcgtcgccgg cgacgaactc ggcctcgagg ccgtctgcgt cagcccggcg
     7801 ccgagcccgg tccagcagcg agggaacgta gtcgagcccg accacgtcgc aaccccggcg
     7861 agccgccgcc agcgcggcat tgccgctgct cgtcgcgaca tcgaggacgc gctcggtcga
     7921 atggaggtcg agcgcctcga tgagcagctc cgacacgatc gtgatctgcg tcccgatcat
     7981 gtggtcgtcg ccgctcgacc aggttgcctg ctggcgctgc ttgatcgcgc ccaggtccgg
     8041 tgccggcgtg gtctcgatgt accggccccg tgggtcggtc gccgtggcgt tcgcgatggt
     8101 catgggatac ccctctgtcg tagcctcgct cgcgttcgga gcgcgggggc gaaagcctcg
     8161 gcctaaaggg ttggcgccga catgccccgt gttcgctacc ttcggcgagc cgggccttca
     8221 tcttcgcgac cctgcgagat gtcagcgggc gcgggaacgg cgctcgcgct caatcggcgg
     8281 acggctcgat cgcgcgtggc tcgatcgcgg gtgtctccat cgccaggtcg gccggcggcg
     8341 actccagatc gctcctcgcg ttcgcggcgg cgcgatcagc gctgcgtcgt tcaatctcca
     8401 cgatgatcca gacgagcagg agcccggcga cgacctgggc gaccgccgcg cccgtgatcc
     8461 atgtgagcag cgagctcaac gccggtgccg tctctggcgg ctgcgtgaag gcctggacga
     8521 tccggttggt gagcccgcca cccagccaca ggatccacca gatgagcacc agacccgcgt
     8581 tgcgctgggc cggccggccc gccaggcgcc gccacgcgtc ggcgacgatc cggtacggcg
     8641 cgacgaagct cgcgaggggc acgaaccaca gggcgatcga ccagcgcggc gtgacccacg
     8701 ggtctgtgct cgaaagggcc gggatgttgt cgacgacacg cgactgccac gcgaggaagg
     8761 cgatcgcggc ggggacgatc gaggcgatgt acagcccact gacggtgttg aggttgcgct
     8821 cccaggtcgt gatctcggcc agcgtcacgc tgccgaagtg ctccacgaag gagaagccac
     8881 gagcaacgac gaggatctcg agggcgatcg cggccgtcgc caggccgatg aagagcatcg
     8941 cccagtgtga ccgccgccgg gcagaccgat accgcggatg gttctggcgg acgatggtct
     9001 gccagtcggt cgcctccatt cgagcctccg tttacttacc ctcacccggc cgagcggcgt
     9061 cgcggcggaa gcatggcatc atcgacctcg aagatcccgc gcaggagcac ctgatgtccg
     9121 tctggttcgg ttaccacatg cccagcttca ccttccccgg cgtgccggcc agcgggctgt
     9181 tcgaccatct cgcgaccctc gccagcgctg cggaggatgc cggattcagc ctcgtgacgg
     9241 tgatggacca cttctatcag atcatcgggg tcggcccgga gaccgacccg atgctcgagg
     9301 cgtataccgc gctcgccgcg ctgagccagc gaacgcgccg tgtccgtctc ggcgcgctcg
     9361 tgagcggggt cacgtatcgg aacccggcgc tcctggcgaa gacagtgacg accctcgacg
     9421 tcctgtcggg cggacgagcg ctgctcggca tcggcgccgc ctggaacgag agcgagcatg
     9481 agggctacgg attcgacttc ccacgcgtgg gcgagcggat ggaccgtctc gatgaggccc
     9541 tcacgatcat ccgggcgatg ttcaccgaag agcgcccgac attcgatggc gagcattacc
     9601 ggatccacga cgcgctgaat gtgccccgcc cgatccagag cggcgggccg cggatcctcg
     9661 tcggcggggg aggggaacag cgaaccctcc ggatcgcggc gaagcatgcg gacctgaccc
     9721 actggttccc cctcggcctc gagacgctgc ggcacaagaa cgaggtcctc gagggctact
     9781 gcgccgagat cggtcgggat cccgccacga tcgagcggac catggcaacg ccggtcctgg
     9841 tcgcgccgaa cgaggcggcg gcgaaggcca tgctcgagcg gatcccgccc gagcgtcgcg
     9901 gccatgtcga gatcggcccg cccgagcagg cggccgaagg cctgcggccc tacatcgagg
     9961 cagggttcac cggtttcacc ttcaacaacc cgattgtctc gacccccgag tcgatccacg
    10021 ccgtcggcga gctgcttcgg ctcatcgcct gacgccccga gcgtcgaccc gggcggtccg
    10081 ccgacggagc gcccgggtca ggccaagcgg gtccctgcgt cagccgatga ggtagacgac
    10141 cccgaccgtg atcgtgacgt cggtcgtccc ggcctggacc ggcgtagcga cgtcgcgtgc
    10201 tgcggctccg gctgtcgcgg ccccgtagaa gatcgggtac ggggtcgggg aggacgtctc
    10261 gctgatcgag acaacaccgg tgatcgctac gccggccgct gcggcgaggg tgtcggcatg
    10321 ggccctcgcc tggctcatcg cgtcggtccg agcctgctgc tcggccttgg ccggatcatc
    10381 gacgcggaac gacacgccgt cgagcgtcgt ggccccggcg gcgagagagt cgtcgatcgc
    10441 gtcgccgacc ttgtcgaggt cgcgcaccgt caccgccacc ccgttcgaga atgaatagcc
    10501 ggtgatccgc ggtgggttcg tgttggtggt gtagtcgtag gtcggctgga gggagatggt
    10561 cgatgtccgg atgtccttgt cggcgatgcc gagggccttg agcgccgcga tcaccttggt
    10621 catcgcggct gcggcatcgg cccgggcggc cttgacggtc ggtcggttga acgtgacgcc
    10681 aaggcgaagg tcggcgacgt cgggagcgac gaccaccgtc ccgacaccgg acaccgagat
    10741 cgtgtgttga ggcggagagg tcgggtccgc agcgagcgtc gagcgcggac cgagcgacgg
    10801 cgacgcgatc gtcgcgacga ggagcccggt ggcgagacca gccgcgatcc agcgggcgcg
    10861 agagccaggg agtcggacgg tcagggtgat cgcatccatg aggagtgtct ccgggaggcg
    10921 gttcggagaa ccgcgcttgg cccggagacg ccagacgagg cgccggggtt tccgatctgt
    10981 cggcgttcca tcgtcacgga ccgttccgcg cggttgcccg caggtctttc cagaaatagg
    11041 tcgtcggggc gagcaccccg tccgagcggt aggcgtggtt cggcatcgtg ccgagctcgt
    11101 gccagccgag cgagcgatag aaggcatcgg ccgcgctgcc tgcctcggtg tcgagaatca
    11161 ggagccaccg gccgtccgcg cgggcacgtt cctcggtggc cagcatgaga gcccggccga
    11221 ggccgcgacg gcgggccgtg cgttggacga ggaccttgcc gacctcggcc cggtgcgggg
    11281 agttggcgtt gcgcgatcgg atgagcaggg tcgagccgac gacggcgccg ggcccgcctt
    11341 ccccggtctc gtcgggcagg cgggcgacga atgccgtcgt ggatccgtcg gcaatctcgg
    11401 gtatccgtcc cgtccaccac gccgtcgctt cctgctcggt caccccggcc atgaagttga
    11461 ccgaggcgcc tcccgcgacc gcatcgatca cgagcaacgc caggccgggg atcgccgcga
    11521 cgtaggcctc ggcgtcgagc gcggcgatcg tcatctggag ccttgccgga agttcaatcg
    11581 tcacgcccgc atcttcgccg acatgagagg gcggcgcgac gggcgcgcct gagggcgacg
    11641 cgatcggtgt cgcgagccgg gcgcgcgtca acggccgccc gcgacgggcg cgtcgccagc
    11701 gtcgagcctg cgatcaccag tccgaagccg atcgccatcg ccggggtgag cttctcgtgg
    11761 aggacgagca cgccgaggac agccgccacg gccgggttga cgtaggtgat gaccgtcgcc
    11821 cggaccgggc cgatctcggc gatgagcgcg gcgaacacga ggaacgcagc agccgtacag
    11881 acgacggcca ggatcgcgat cgagccgacc acgttcacgg acgggacgat cgacggccac
    11941 tggaaggccg tgatcggcac gtacgccgct gcgcagaggg caagcgagat cgccatcacc
    12001 ccgacggacg ggagcccgcc gagacggcgc gccaggatgg ccggcccgac cgcatagcag
    12061 acgaccacga ccccgatctc gagcagggct gaaggatccg atgtcgcgaa gtcgccccag
    12121 acgatcgccg cgacgccgac caggccgatc ccgaggccga cgaggcccgt ccggccgagc
    12181 cggtcggtcc cgccggtggc gagcgcgaac gtcgtgccga cgagcgggac gccggcgatc
    12241 aacagccccg caagcgagct cgagacatgc tgctccgcag atccgagcgc cacccacgga
    12301 agggcgatct cgattgcggc gaacgcgacg acccagcgcc agcgggcaag caccctccgc
    12361 aggtcgaccc gggcgatggc gtagggaagc aggatcgccg cggcaatcga cgtgcgggcg
    12421 aggacgagtg cggccggcga gatctcggcg accgcgaccc ggatgaacag gtaggggatg
    12481 ccccagatga ccgacatcag gccgaagagg acgagcccgc gtcgcgtcac ccgaacaggc
    12541 tcgggcacga cgtcacgccg gcgatcttcg cagaattccc gccggccggt cggcaaccgt
    12601 gatccgatcg aaacgctgcc gttgtcggac aatggcgcgg tggcgcacga tctcgagcgg
    12661 ttcctcgtcg cccaggagta cctctacgac ggcgtcctcg atgagctacg gcgcggacag
    12721 aagacgggcc actggatctg gttcatcttc ccgcagatcg caggtctcgg acgcagcgcg
    12781 ctgtcgcaac agttcgccat cagctcgctc ggcgaggcgc gcgcctatgt cgcccatccg
    12841 gtcctgggag tgcgcctgcg cgaatgtgcg gcggccctgc tggcgacgac ccctcggacc
    12901 gcggagcaga tcctcggttc gatcgacgcc atgaaggtgc gctcctgcat gaccctgttc
    12961 caccgggcgg cgcccgacga gccggtgttc agccaggtgc tcgaccgcta ctacgctgga
    13021 gcggctgacg aggcgacggt
//
LOCUS       DATAIW010000235        12809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_162360, whole genome shotgun sequence.
ACCESSION   DATAIW010000235 DATAIW010000000
VERSION     DATAIW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162360"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VHM48_07460"
     CDS             <1..237
                     /locus_tag="VHM48_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755283.1"
                     /translation="LLPSLREYAARYGLTRDRLALARPDALVMHPGPMNEGVEIAAGR
                     RVNEPGARDLEVGHDRADRGSGSDRHGRAVPASR"
     gene            complement(207..1244)
                     /locus_tag="VHM48_07465"
     CDS             complement(207..1244)
                     /locus_tag="VHM48_07465"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2755284.1"
                     /translation="MTLIDREPQAQEALEVPVVDSPPDAQPVGWHHRHLLDVNALSWP
                     DIELVMRTTDAMREVLARPIAKVPALRGRNVTILFYEASTRTRVSFEVAAKNLSADVV
                     NIAAATSSVAKGESLIDTVKTVEALGAQMLVMRHSVSGAPYLAAEIFSGSVLNGGDGW
                     HAHPTQALLDLYTMRGRFEGGSLVGRKVVILGDILHSRVARSNIWTLTAAGADLWLCG
                     PATLLRGFEAWAGRGAADGRRFHVTSSIDEALRDADVVMALRIQKERMSGGLLPSLRE
                     YAARYGLTRDRLALARPDALVMHPGPMNEGVEIAADVAAGTQSLIEEQVRNGVAVRMA
                     LLYLLAGTARP"
     gene            complement(1241..1804)
                     /gene="pyrR"
                     /locus_tag="VHM48_07470"
     CDS             complement(1241..1804)
                     /gene="pyrR"
                     /locus_tag="VHM48_07470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2755285.1"
                     /translation="MSGRQIMTADEIRRATVRISHEIVEKQAGTSGLALVGIQRRGVP
                     LARRIADAIAEHEGVSVPVGALDITFYRDDLSLVALQPVVKGTDLPFDLNGATVVLVD
                     DVLYTGRTIRAAMDALVDFGRPLAIRLAVLVDRGHRELPIRADHVGKNVPTSREEVVR
                     VQLEEIDGEDSVNIDRAAEAVTAAPVG"
     gene            complement(1940..2401)
                     /locus_tag="VHM48_07475"
     CDS             complement(1940..2401)
                     /locus_tag="VHM48_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755286.1"
                     /translation="MLDPSAFQSVQVPAFASTDLAVEPLDAAHRGAGPIDSTTLLTEP
                     GKEPKRVVPTRPSVAVPGAHSGSALKPPRYKISGFATFYANGTTAMRLPRGTTVIICG
                     AGGCIERVVNDYGPAANAGLGRIVDLYTPDFFAICGCASWSGTARVTVSVY"
     gene            complement(2714..4540)
                     /locus_tag="VHM48_07480"
     CDS             complement(2714..4540)
                     /locus_tag="VHM48_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2755287.1"
                     /translation="MNVVRGRARRPDDWPSSHVRARADLSDRLDSPLDPTEAGWLELH
                     LETCPDCRSSADAYTAQRLELRALRDRIPSPPRDLWARTAAAIESESRFRDNRGRTAV
                     RLGRRALAPSALLAAALVAVVAVGLLTSSQWPFGGDGARSSALIADGAGPSTFTADAT
                     GASPTLIPVTQKIAYLSRESGGSFSYKTKNVDKVCPTTSPGPCDDTNATVDRPVKLDP
                     NASTVFGSRDDKRLIVINDPSSASSGTISVVPLGSEPPDSTPVPSATPTPASATPASA
                     PPTTGPTATPTVTPAVGPGSPSPSATPTASVSPSPSVVVTPSPSGAIEIAHDVDLVGQ
                     SAAYSPSGDWFAFTARPVNGTFGPDIYVWKVGEPMANRVTGDHRSVFGSWSGEIMVGS
                     TIFAITKGGSTALRTDLVPASYLLDPATTTVTSLPQTGQAWRPAVDPSGRLAVYWAGT
                     VRATTTPGFAPDAGRLVLGDWSTGTSAPTDGALPTSLVGDQGAARHETTIEAGRIDDW
                     DARWDSTGTHLAVWIANAQNPAVGRLSLYSVDSFDGKIDLKAPLLDGKPAIAGYSISK
                     GQLVWAEPSGEGSTAGKILLLAWTDEGVGTVGTVTGPVIVIR"
     gene            complement(4537..5187)
                     /locus_tag="VHM48_07485"
     CDS             complement(4537..5187)
                     /locus_tag="VHM48_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2755288.1"
                     /translation="MVGALDTRIGVSRGFGGRAAMSGAWEHDAAAGFVEDLFAAHHGE
                     IYAYLIRMLRDPELAADLTQDAFIKAYRNYNVLEKPENARAWLYQIAHRVALDHIRRQ
                     KIVRFLPWTGESHGAAPSTERLVMDAHLSGDLQRALAKIPERQRAALLLAEIHDLTGL
                     ELAAALGVSHVAARALLTRARESLRQALAVERQADAEAEADAASAAGTDRHSGTAR"
     gene            complement(5309..6313)
                     /locus_tag="VHM48_07490"
     CDS             complement(5309..6313)
                     /locus_tag="VHM48_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755289.1"
                     /translation="MAAGLFELRKDAITGWWVATVVDRAFHRDRFARAADPIDDGGDC
                     QNCRLPAGDGVRIRTLKDFAFHLVGTDEQARELDGSVAQVSLQDARASGSWRTIVAPP
                     GEHRPLHAVGSGTIVALLTAARTALIDARRTALTDHLQVVQNWGGQAGARTNHLCLDL
                     YDLPQIPHRIAEELGGSARFVIREGECPWCRLVRDESNRPERLIWQDNDTVAFAPFAS
                     RSPFEVWVVPRRHDADFGRASDGDIAATGEALRQVLGSLSVTLDGPPYNLVLHTSPLR
                     EQVDATYHWHWEIHPRLREIAGLELGTGLPVNPVSPEDAVEELLVRTRRGSTGAGASG
                     "
     gene            complement(6315..7115)
                     /gene="lipB"
                     /locus_tag="VHM48_07495"
     CDS             complement(6315..7115)
                     /gene="lipB"
                     /locus_tag="VHM48_07495"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX2755290.1"
                     /translation="MDSTARRDDFRPIDRPETGPLEAVWLGRADYRSVWALQKELAAA
                     RARGAIGDRLLLVEHAAVLTLGRQADESHVRATPTELAARGIELLRVERGGEVTYHGP
                     GQLVAYPIIALSRRGLLLRPLVRALEAAMISTCAAFGVDTARRDGHPGCWIDPGGPAR
                     RKIGALGLRVERGVSYHGIALNVDPDLDDFDLIDPCGMPGVVSTSIAAEAGRPRERPS
                     TSAVAEAAVVFARAFAGQIGVVLDGFGSAGAEGSSSASDPSGALATVG"
     gene            complement(7181..7957)
                     /locus_tag="VHM48_07500"
     CDS             complement(7181..7957)
                     /locus_tag="VHM48_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755291.1"
                     /translation="MATLVAAALATTLAGCDLMIATTTPRPSRLIATPEPTPSPTPTE
                     LDESPTLRPDPSGTGPDLLDSANALADLDSYRVSIVSRGLVPATPAGGAVTMTSTLIQ
                     GSEPAAEFSMAGVDGFAAGRLRAVVIGDRAWLKEGTGPWVKSPGGAADFDAAFTTLSP
                     IDLVGGFDGLSAALRRIGPASRNGQRTIHYHTDSGDAAAAAAGLTNGSVDAWLATRGG
                     DLVSLAIDGTWDLDGTPTRVVLMIEVTRINDRTNNVAPPI"
     gene            8182..9174
                     /locus_tag="VHM48_07505"
     CDS             8182..9174
                     /locus_tag="VHM48_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2755292.1"
                     /translation="MGTVPSHRAHAPRSRISFRLAGLATLVGFVVLAAACGGGPSRSS
                     APSADPTKDKLAQVLARGTLVLSTDLDYAPQSFAVKGATRAASSKCGPNQLTAPEVSG
                     YDAETGKLVAAAMGVEPCFVDVPFDQVIAGNWGDRWDVAWGSGALTTSRMKALYVTQP
                     YYSTPANYFVLASSKYQAPADLAGKQIGACAGCTHELYLRKTLSLPGVTLTLDVTDPK
                     IVTFASEVPGLDALAAGKIDAFLCSQPVGAGAIADGKPVRMLEKTAFFTQKTGYVDRG
                     LTLVPGPFLDRVNAAIRDLHAQGKLKALSLDAFKTDYATSAAAFDLASVGQKIP"
     gene            9193..12423
                     /locus_tag="VHM48_07510"
     CDS             9193..12423
                     /locus_tag="VHM48_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2755293.1"
                     /translation="MADRISRPWRRSLRSRLVAYFVVLSAITILVVGGVVYIRATDDL
                     THAVYDRLDSVAANKADSLDRWVDEQTRNVVYVGSIPGFGDDARAYLDPAGAAATRSA
                     ADARLRQDLLTIVRGTTDAEEIFILDLGGTIRLSTLAEHEGSTQANEAFYVDGSSHTT
                     VQNVYTSTLTSRPTITVATPLFDQNGGGQRVAVLAANLSLARVDRIILERTGLGDSGR
                     TYLVGTDHRLIQGANAQGAQGIVDSEGIRNVLEPRSGQGLYADDRGTPVVGVYRWLGN
                     RGAGLLAEISQDEALGSARQLALTIGLVGLVSTLLLAAGIWVVARRVTRPILSLAATA
                     SRVAGGDLDAVSGIRSDDEVGALATAFDGMTAELRENVATLERRVAERTAELTRQRLY
                     YASLVEVSPVAVVTMDLEERVSAWNPAATTLFGYVPDEAIGKRIDELILRSDELRAEG
                     QDLAREALDGRVQRIGHRMRKDGSLVDVEILMVPLVVDDERLGFYAIYHDISELQAAR
                     READDANQAKSSFLAAMSHEIRTPMNAVIGMSGLLMDTPLDAEQRDYADTIRTSGEAL
                     LTIINDILDFSKIEAGRIELDAAPFGLAGTIEGALDVMGPIAAKKGVELVYAVDPDLP
                     ATVIGDSGRLRQIVLNLLSNAIKFTEQGEVVLSVNGGRDPAAEVTAAAGAEPSDPTRS
                     TWELRLDVHDTGIGIPPDRIGRLFQSFSQADASISRRFGGTGLGLAISRRLAELMGGS
                     LSAESTGIAGEGSTFHMVIHLSEAGEPSAGATAGSPDAEATAGARAGIVGRRVLVVDD
                     NATNLRILVAQLERLGLDVTATTHSREALDLATVAPADFAAVLSDYRMPDLDGAGLAA
                     AIHAAAMPSPPPVVILSSAGQRERDAPGVAAFLSKPVKPGPLRDAVVAVLTGHAVQEA
                     VRPERSATDPELGVRHPLRILLAEDNAVNQKLALRLLERMGYTADVAVNGLEAIEALE
                     RAPYDVVLMDVQMPELDGLEASRRIRSRWPDGNLRIVAMTANAMEGDRDACIAAGMDD
                     YVSKPIRPDELAAALAAAGPPEHGSPDARRAAAARSIAETDAAATA"
BASE COUNT         1936 a         4470 c         4590 g         1813 t
ORIGIN      
        1 ctgctgccgt cgctgcgcga gtacgcggcg cgctacgggc tgacgcgcga ccggctggcc
       61 ctggctcggc cggacgcgct ggtcatgcat ccggggccga tgaacgaggg cgtcgagatc
      121 gcggccggac gccgggtcaa cgagccaggc gcgcgggatc tcgaggtcgg ccacgaccgc
      181 gccgatcgcg gatccggcag cgaccgtcat gggcgggccg tcccggcgag caggtagagc
      241 agcgccatcc ggacggcgac cccgttgcga acctgctcct cgatcagcga ctgggtcccc
      301 gccgcgacgt cggccgcgat ctcgacgccc tcgttcatcg gccccggatg catgaccagc
      361 gcgtccggcc gagccagggc cagccggtcg cgcgtcagcc cgtagcgcgc cgcgtactcg
      421 cgcagcgacg gcagcagacc gccggacatc cgctccttct ggatccggag cgccatgacg
      481 acgtcggcat cgcgcagcgc ctcatcgatc gacgacgtga cgtggaaccg ccgtccgtcg
      541 gcggcgcccc gcccggccca tgcctcgaag ccgcggagca gcgtcgcggg gccgcacagc
      601 cagaggtccg ctccggccgc ggtcagcgtc cagatgttcg agcgggcaac ccgggagtgg
      661 aggatgtcgc cgaggatcac gaccttgcgg ccgacgagcg agccgccctc gaaccggccg
      721 cgcatcgtgt acaggtcgag cagggcctgg gtcgggtggg cgtgccagcc gtcgccgccg
      781 ttgaggacgg agcccgagaa gatctccgcc gcgagatacg gcgcaccgga cacggaatgc
      841 cgcatcacga gcatctgggc cccgagcgcc tcgaccgttt tgaccgtatc gatgagcgac
      901 tcgcccttcg cgaccgacga ggtcgccgcg gcgatgttga cgacgtcggc cgagaggttc
      961 ttggccgcga cctcgaacga cacccgggtc cgggtcgacg cctcgtagaa gaggatcgtc
     1021 acgttccggc cacggagcgc cgggaccttc gcgatcgggc gggcgagaac ctcgcgcatc
     1081 gcgtccgtcg tccgcatgac cagctcgatg tccggccacg acagcgcatt gacatcgagc
     1141 agatggcgat ggtgccagcc gaccggctgt gcgtccggcg gcgaatcgac aaccgggacc
     1201 tcgagggctt cctgcgcctg gggctcccga tcgatcagcg tcatccgacc ggcgccgcag
     1261 tcacggcctc ggccgcccgg tcgatgttga cgctgtcctc gccgtcgatc tcctcgagct
     1321 ggaccctgac gacctcctcg cgggaggtcg ggacgttctt gccgacgtga tcggcccgga
     1381 tcggaagctc gcggtggccg cggtcgacga gaaccgcgag gcggatcgcc aatggccgcc
     1441 cgaagtcgac gagggcatcc atcgccgccc ggatcgtccg cccggtgtag aggacgtcgt
     1501 cgacgagaac gacggtcgcg ccgttgaggt cgaagggaag gtcggtgccc ttgacgacgg
     1561 gctgcagggc gaccagcgac agatcgtcgc ggtagaacgt gatgtcgagg gcgccgaccg
     1621 ggacggacac cccttcgtgc tcggcgatcg catcggcgat ccgacgggca agcggaacgc
     1681 cacggcgctg gatgccgacc aaggccagac cgctcgttcc cgcctgcttc tcgacgatct
     1741 cgtgcgagat ccggaccgtc gctcgacgga tctcgtccgc cgtcatgatc tgccgaccgc
     1801 tcattgctcc ttcccgggcc tcgcggggct ctcatgcggc ctcacggggc cgctcgaagg
     1861 gtcgcgagaa ggatacccga cggggaggcc gctccgggcg cgaccgggcc gccgactcgg
     1921 gtcccggcgg cgtgcccgct cagtagaccg agacggtcac ccgcgccgtc cctgaccacg
     1981 acgcgcagcc gcagatcgcg aagaagtccg gggtgtacag atcgacgatc cgaccgagcc
     2041 cggcattggc cgcgggcccg tagtcgttga ccacccgctc gatgcagccg ccggcgccgc
     2101 agatgatcac ggtcgtcccc cgcggcagcc gcatcgccgt cgtgccgttg gcatagaacg
     2161 tcgcgaagcc actgatcttg taccgcggcg gcttgagggc ggacccactg tgagcccccg
     2221 gcacggcgac ggatggtcgg gtcgggacga cccgcttcgg ctccttgccg ggctccgtca
     2281 ggagggtcgt cgaatcgatc gggccggcgc cacgatgggc ggcgtcgagc ggctcgaccg
     2341 cgagatcggt cgacgcgaac gccgggacct ggaccgactg gaacgctgac gggtcgagca
     2401 cagaggcggg cgttggcccg gcagatccgg cgatacccgg caacgcaccc agggcgaggg
     2461 cggcggtgag cgccgcgcct gcaaccatac gggagggtcg cacttgcacc gaacaataca
     2521 agatcgtcgg ctatcgcgct ggaacgatga gggcggcgtt gccacctcgg gccgatggcg
     2581 tcgcggtgct ccgccgcgga cctcggtgag cccagaggcg gcgccgtgcc atcctcgccg
     2641 gcgagacgaa cgtcagtggc gggctctctc gccttcgcga cgcacgaggc cgcggctcga
     2701 ggaccgcgac gggtcagcgg atgacgatga ccggccccgt gacggtcccg acggtcccga
     2761 ccccttcgtc ggtccaggcg agcaacagga tcttgccggc cgtcgagccc tcgccggacg
     2821 gttccgccca gacgagctgg cccttcgaga tcgaataccc ggcgatcgcc ggtttgccgt
     2881 cgaggagcgg tgccttcagg tcgatcttgc cgtcgaacga gtcgaccgag tagaggctca
     2941 ggcggccgac agccgggttc tgcgcgttag cgatccagac cgcgaggtgg gtcccggtgc
     3001 tgtcccagcg cgcgtcccag tcatcgatcc gaccggcctc gatcgtggtc tcgtgacggg
     3061 ccgcaccctg gtcgcccacg agggacgtcg gcagcgcgcc atcggtcggc gcgctggtgc
     3121 cggtgctcca gtcgccgagg accagccggc cggcgtcggg tgcgaaaccc ggcgtcgtgg
     3181 ttgcccggac ggttcccgcc cagtagacgg cgagacggcc ggacggatcg accgccgggc
     3241 gccaggcctg gccggtctgc ggcagggagg tgacggtcgt cgtggcggga tcgagcaggt
     3301 aggaggccgg caccaggtcg gtccgtagcg ccgtgcttcc gcctttcgtg atcgcgaaga
     3361 tcgtgctgcc gaccatgatc tcgcccgacc aggacccgaa gacggagcgg tgatcgcccg
     3421 tgacccggtt ggccatcggc tcgccgacct tccagacgta gatgtccgga ccgaacgtgc
     3481 cgttgacggg acgggcggtg aaggcgaacc agtcgccgct cggcgagtag gccgcggact
     3541 ggccgacgag gtcgacatcg tgggcgatct cgatcgcacc gctcggggaa ggggtcacca
     3601 cgaccgacgg agacggcgag acgctggcgg tcggggtcgc gctcggcgac ggacttcccg
     3661 ggccgacggc tggcgtcacc gtcggagtgg cggtcgggcc cgtcgtcggc ggtgccgatg
     3721 ccggggtcgc cgacgcgggc gtcggcgtcg cgctcgggac cggcgtcgaa tcggggggct
     3781 cgctgccgag cgggaccacg gaaatggtgc cgctgctcgc ggaggaggga tcgttgatca
     3841 cgatcaggcg cttgtcatcg cgcgagccga agacagtcga cgcgttcggg tcgagcttga
     3901 ccgggcgatc gactgtggcg ttggtgtcgt cgcacggccc gggcgatgtc gtcgggcaga
     3961 ccttgtcgac gttcttggtc ttgtacgaga aggagccgcc cgattcgcgg gagagatagg
     4021 cgatcttctg ggtgaccgga atcaaggtcg gactcgcgcc ggtggcgtcg gcggtgaacg
     4081 tggacggccc cgcgccatcg gcgatcaggg ccgacgacct cgcgccgtcg ccaccgaagg
     4141 gccactggga ggaggtcagc agtccgacag cgacgacggc gacgagagcc gccgcgagaa
     4201 gagcagaagg tgcgagcgcg cggcggccga gccgaacggc cgtccggcca cggttgtcgc
     4261 ggaacctgga ttcggattcg atcgcagccg ccgtgcgcgc ccagaggtcc ctcggcgggg
     4321 acggaatccg gtcgcgcaga gcccgaagct cgagccgctg ggcggtgtac gcgtcggcgc
     4381 tggaccgaca gtccggacac gtctcgagat gaagctcgag ccagcccgcc tcggtcggat
     4441 cgagcggtga gtccagacga tccgacaggt cggcgcgagc ccggacgtgg ctcgagggcc
     4501 aatcgtcggg gcggcgggcg cgtccgcgaa cgacattcat cgggctgtgc ccgaatggcg
     4561 gtcagtgccg gctgccgacg ccgcatcggc ctcggcttcg gcgtcggcct ggcgttccac
     4621 ggcgagggcc tgacgaaggc tctctcgcgc tcgggtcagc agcgcccggg cggcgacgtg
     4681 gctgactccc agagcggcgg cgagctcgag accggtcagg tcgtggatct cggcgagcaa
     4741 cagggcggcc cgctggcgtt cggggatctt cgcgagggcc cgctggagat cgcccgagag
     4801 gtgggcgtcc atgaccaggc gctcggtcga gggtgcggcg ccgtggctct cgccggtcca
     4861 tggcaggaag cggacgatct tctggcggcg gatgtggtcg agggccacgc gatgggcgat
     4921 ctggtacagc caggcccggg cgttctccgg cttctcgaga acgttgtagt tgcggtaggc
     4981 cttgatgaag gcgtcctggg tgagatcggc ggcaagctcc gggtcgcgaa gcatgcggat
     5041 caggtaggcg tagatctcgc cgtgatgcgc cgcgaacagg tcctcgacga acccggcggc
     5101 ggcatcgtgc tcccaggcgc cgctcatggc cgctcgccca ccgaatcccc ggctgacgcc
     5161 gatccgggtg tcgagcgcgc cgaccatgca gcctccgagt ccagtgatga cgggatcggt
     5221 gcgagcggcg atcgccgtgc gctccgtggc ccagagacga ggtggctcat cgatgtgtga
     5281 cgccgtccgc ccctcggcgt tacatccatc agcccgaggc ccccgcaccc gtcgacccac
     5341 gccgcgtccg gaccagcagc tcctcgaccg cgtcctcggg tgacaccggg ttgaccggaa
     5401 ggccggtccc gagctcgagg ccggcgatct ctcgcaggcg agggtggatc tcccagtgcc
     5461 agtgataggt cgcgtcgacc tgctcgcgca gcggcgaggt gtgcaggacg aggttgtacg
     5521 gtgggccgtc gagcgtgacc gacagcgacc cgagaacctg gcgcagcgcc tcgccggtcg
     5581 cggcgatgtc gccgtccgaa gcacggccga aatcggcgtc gtggcggcgc gggacgaccc
     5641 acacctcgaa cggtgagcgc gacgcgaacg gtgcgaaggc gacggtgtcg ttgtcctgcc
     5701 agatgaggcg ctccggacgg ttcgactcgt cgcggacgag ccggcaccac gggcattcgc
     5761 cctcgcggat cacgaaacgg gcggacccgc cgagctcctc ggcgatccgg tgcgggatct
     5821 gcggcaggtc gtacaggtcg aggcacagat ggttggtccg tgcgccggcc tggccgcccc
     5881 agttctggac cacctggaga tgatcggtga gggcggtccg ccgcgcgtcg atcagggccg
     5941 tccgggcggc tgtgaggagg gcgacgatcg tcccgctccc gacggcgtgg agcggccggt
     6001 gctcaccggg cggagcaacg atcgtccgcc agctgccgct cgcccgggcg tcctggagcg
     6061 agacctgggc gacgctgccg tcgagctcac gcgcctgctc gtcggttccg acgaggtgga
     6121 acgcgaagtc cttgagggtc cggatccgca ccccgtcacc cgccggcaga cggcagttct
     6181 ggcagtcgcc accgtcgtcg atcgggtcgg cggcgcgggc gaagcggtca cgatggaagg
     6241 ctcggtcgac gacggtcgcg acccaccacc cggtgatcgc gtccttccgg agctcgaaga
     6301 gaccggcggc cacctcagcc gaccgttgcg agtgcgcccg atggatcgga cgcggacgac
     6361 gaaccctcgg cgccggcgga tccgaagccg tcgagcacga ccccgatctg tccggcgaac
     6421 gcccgtgcga acacgacggc ggcctccgca accgcggacg tcgagggccg ctctcgcggt
     6481 cgaccggcct cggccgcgat cgaggtcgag accacgccgg gcatcccgca gggatcgatc
     6541 aggtcgaaat cgtcgagatc ggggtcgacg ttcaaggcga tcccgtggta gctcacgccg
     6601 cgctcgacac ggagccccag cgctccgatc ttgcgccgcg ccgggccgcc aggatcgatc
     6661 cagcaccccg gatggccgtc ccgtcgagcg gtgtcgacgc cgaacgcggc gcaggtcgag
     6721 atcatcgccg cctcgagggc ccggaccagg ggccggagca gcagtccgcg tcgtgacaga
     6781 gcgatgatcg ggtaggccac cagctggccc gggccgtgat atgtcacctc gccgccgcgc
     6841 tcgacccgga ggagctcgat cccgcgcgcc gccagctcgg tcggggtcgc acggacgtgg
     6901 gattcgtcgg cctggcgtcc gagcgtgagg acggccgcat gttcgacgag gaggagccgg
     6961 tcaccgatcg ctccgcgggc tcgagcggcc gcgagctctt tctggagcgc ccagacggag
     7021 cggtagtctg cacgaccgag ccagaccgcc tcgagcgggc ctgtctcggg gcgatcgatc
     7081 gggcggaagt cgtcgcggcg ggctgtcgag tccaccgccg cacgatagca ggggaaggcc
     7141 cgcgggccgt tcgtcgacgc gatccgctcc ctgcgttgcg tcagatgggt ggcgcgacgt
     7201 tgttcgttcg atcgttgatg cgggtgacct cgatcatcag gacgacccgg gtcggcgtgc
     7261 cgtcgaggtc ccacgtcccg tcgatggcca ggctcacgag gtcgccaccc cgcgtcgcga
     7321 gccacgcgtc gaccgaaccg ttcgtcaggc cggccgcggc ggcggccgca tcacccgagt
     7381 cggtgtggta gtggatggtc cgctgaccgt tcctgcttgc cgggccgatc ctgcggagcg
     7441 cggccgacag gccatcgaag ccgccgacga gatcgatcgg tgagagcgtc gtgaacgccg
     7501 catcgaaatc ggcagcgccg cctgggctct tgacccacgg accggtcccc tccttcaacc
     7561 acgcccgatc gccgatcacg accgcccgca gacggccggc cgcgaagccg tcgacgccgg
     7621 ccatgctgaa ttccgccgcg ggctcgctgc cctggatcag ggtcgacgtc atcgtcacgg
     7681 cgccaccggc cggtgtcgcc gggaccaggc cgcgactcac gatggagacg cggtacgagt
     7741 cgaggtcggc gagggcgttc gcggaatcga gcagatcggg gccggtgccg gacggatccg
     7801 gacgcagcgt cggactctcg tcgagttcgg tcggcgtcgg cgatggcgtc ggctcggggg
     7861 tggcgatcag ccgtgatggg cgtggagtcg tcgttgcgat catcaggtcg cagccggcca
     7921 gcgtcgttgc gagggccgct gcgaccaggg tcgccacgaa ggggatgcga cggacgaacg
     7981 aggaacgtgg acgcatcgat gccccggtgg ggagcgaggg aacggtgaac gggagcatag
     8041 caaggctgtg ccggcgaccg gctccggcgg tcgttggatg accgacagat gcgaaagggc
     8101 cgttcacgac ggcaaatcat ctcgtaccat cgccggactc gccgggtcga accgtcactt
     8161 gccggggacg aaggagttcg cgtgggcact gttccgagcc accgcgcgca cgcgccacgg
     8221 tctcgcatct cgttccgcct tgccgggctg gcaacgctcg tcggtttcgt cgtcctcgcc
     8281 gcagcatgcg gcggtgggcc gtctcgctcg tcggctccga gcgcggatcc gaccaaggac
     8341 aagcttgccc aggtcctggc gcgggggacc ctggtcctct cgaccgacct cgactacgcc
     8401 ccacagtcct tcgcggtcaa gggcgcaacc cgggccgcct cgtccaagtg cggcccgaac
     8461 cagctgacgg cgccggaggt ctccggctac gacgccgaga ccgggaagct ggtcgcggcc
     8521 gccatgggcg tcgaaccctg cttcgtcgat gtgccgttcg accaggtcat cgccggcaac
     8581 tggggggacc gctgggatgt cgcgtgggga tccggcgccc tgacgacctc ccggatgaag
     8641 gccctctacg tgacgcagcc ctattactcg acgcccgcga actacttcgt cctcgcctcc
     8701 tcgaagtacc aggcgcccgc cgacctggcc ggcaagcaga tcggcgcctg cgcggggtgt
     8761 acgcatgagc tctacctccg caagaccctg tcgctgcccg gcgtgaccct gacactggac
     8821 gtgaccgacc cgaagatcgt cacgttcgcg agcgaggtgc ccggcctcga cgcactcgcc
     8881 gccggcaaga tcgacgcgtt cctgtgcagc cagcctgtcg gggccggcgc aatcgccgac
     8941 ggcaagcccg tccgaatgct cgagaagacc gcgttcttca ctcagaagac gggctacgtc
     9001 gatcgcggcc tgacgctggt gcccgggccc ttcctcgacc gggtcaatgc ggcgatccgc
     9061 gacctgcacg cccagggcaa gctcaaggcc ttgtcgctcg acgccttcaa gaccgactac
     9121 gcgacctcgg ccgcggcatt cgacctcgct tccgtcggcc agaagatccc gtaggggagc
     9181 ggcatcccgt ccatggccga tcgcatcagc cgaccctggc ggcgaagcct ccggtcgcgg
     9241 cttgtcgcct acttcgtcgt cctgtcggcg atcacgatcc tcgtcgtcgg cggcgtcgtg
     9301 tacatccggg ccaccgacga cctgacccat gcggtctacg accgtctcga ctccgtcgca
     9361 gcgaacaagg ccgactcgct cgatcgctgg gttgacgagc agacgcggaa cgtcgtgtac
     9421 gtcgggtcga tcccgggatt cggcgacgac gcgcgggcct acctcgaccc ggcgggcgcg
     9481 gccgcaaccc ggtccgcggc cgatgcccgg cttcggcagg atctcctgac catcgtccgc
     9541 ggcacgaccg acgccgagga gatcttcatt ctcgacctcg gggggacgat ccgcctgtcg
     9601 accctcgccg agcacgaagg ctcgacgcag gcaaacgagg cgttctatgt cgacggctcc
     9661 tcgcatacga cggtccagaa cgtctacacc tcgaccctga cgagtcgccc gacgatcacc
     9721 gtcgcgacgc ccctgttcga tcagaacgga ggtggccagc gagtggccgt cctggcggcg
     9781 aacctcagcc tcgccagggt cgaccggatc atcctcgagc gaacggggct cggcgactcg
     9841 ggccggacct atctcgtcgg aaccgaccat cgtctgatcc agggggccaa tgcgcagggc
     9901 gcccagggca tcgtcgactc ggaggggatc cggaatgtgc tggagccccg gagcggtcag
     9961 ggtctctacg ccgacgaccg cgggacgccc gtcgtcggcg tctatcgctg gctgggaaac
    10021 cgcggcgccg gcctccttgc cgagatcagc caggacgagg ccctcggatc ggcgcgccag
    10081 ctcgccctga cgatcggcct cgtcggtctc gtctcgacgc tcctgctggc agccgggatc
    10141 tgggtcgtcg cccggcgggt cacacgcccg atcctgtcgc tcgccgcgac cgccagccgg
    10201 gtcgccggtg gcgacctcga cgcggtgtcc gggatccgct ccgacgacga ggtcggggcg
    10261 ctcgcgaccg cgttcgacgg gatgaccgcc gagctccgcg agaacgtcgc gactctcgag
    10321 cggcgcgtgg ccgagcggac ggccgagctg acccggcaac gcctctacta cgcgtccctg
    10381 gtcgaggtca gcccggtcgc cgtcgtcacg atggacctcg aggagcgggt ctcggcctgg
    10441 aacccggcgg cgacgacgct cttcggctac gtgcccgacg aggcgatcgg gaaacggatc
    10501 gacgagctga tcctgcgctc cgacgagctc cgagccgagg gccaggacct cgcccgcgag
    10561 gcgcttgacg ggcgcgtcca gaggatcggc catcggatgc gcaaggacgg ctcgctcgtc
    10621 gacgtcgaga tcctgatggt gccgctcgtc gtcgacgacg agcgcctcgg gttctatgcg
    10681 atctaccacg acatcagcga gctccaggcg gcccgccgcg aagccgacga tgcgaaccag
    10741 gcgaagagct cgttcctcgc ggcgatgagc catgagatcc ggaccccgat gaacgccgtc
    10801 atcgggatga gcgggctcct gatggacacc ccgctcgatg ccgagcagcg cgactatgcg
    10861 gacacgatcc ggacctccgg ggaggcgctc ctgacgatca tcaacgacat cctcgacttc
    10921 tcgaagatcg aggccggccg gatcgagctc gacgcggcac cgttcgggtt ggccggcacg
    10981 atcgagggcg cgctcgatgt catgggcccg atcgccgcga agaagggcgt cgaactcgtc
    11041 tacgccgtcg atcccgacct gccggcgacc gtcatcggcg actcgggtcg gctccgccag
    11101 atcgtcctga acctgctgtc gaacgcgatc aagttcaccg agcagggcga ggtcgtcctg
    11161 agcgtgaacg gcgggcgcga cccggcggcc gaggtcaccg cggcggccgg cgcggagcca
    11221 tccgatccga cccggtcgac gtgggagctc cggctcgacg tccacgacac cgggatcggg
    11281 atcccgccgg accggatcgg gcggctgttc caatcgttca gccaggcgga tgcgtcgatc
    11341 tcgcggcggt tcggcggaac gggcctcggg ctcgcgatca gccggcgact ggccgagctg
    11401 atgggcgggt ccctgtcggc cgagagcacg ggcatcgccg gcgagggcag caccttccac
    11461 atggtcatcc acctgtcgga ggcgggcgag ccgtcagccg gagcgacggc cgggtcgccc
    11521 gatgccgagg cgaccgcggg ggcccgcgcc gggatcgtcg gccgtcgtgt cctcgttgtc
    11581 gacgacaacg ccacgaacct ccggatcctc gtcgcccagc tcgaacgcct cggtctggac
    11641 gtgaccgcga cgacccattc ccgcgaggca ttggacctgg caaccgttgc acccgccgac
    11701 ttcgcggccg tcctctccga ctaccggatg ccggatctgg atggcgccgg gctcgccgcc
    11761 gccatccacg ccgcggcgat gccgagcccg ccgccggtcg tgatcctgtc gtcggccggc
    11821 cagcgcgaac gcgacgcgcc cggggtggct gcgttcctgt cgaagccggt caagcccggt
    11881 ccgctccgtg atgccgtcgt cgcagtgctg accggacatg ctgtccagga ggcggtgcga
    11941 cccgagcggt ccgccacgga cccggaactc ggcgtccggc atccactgcg gatcctcctt
    12001 gccgaagaca acgcggtgaa ccagaagctg gccctccggc tcctcgagcg gatgggctac
    12061 acggcggatg tcgcggtcaa cggcctcgag gcgatcgagg ccctcgaacg cgctccgtac
    12121 gacgtcgtgc tcatggatgt ccagatgccg gagctcgacg gcctcgaggc gtcccgccgg
    12181 attcgatcgc gatggccgga cgggaacctc cggatcgtgg cgatgacggc gaacgccatg
    12241 gaaggcgatc gcgacgcgtg cattgcggct gggatggacg attacgtctc caagccgatc
    12301 cggccggacg agctggcggc ggccctcgcg gccgcgggcc cacccgagca cgggtccccc
    12361 gacgcccgcc gcgcagcggc cgcgcgctcg atcgcagaga cggatgccgc ggccaccgcc
    12421 tgaccgaacg actcccctcg ggcgccgacc agcgcgcccc cacagcagtc acgatcgccg
    12481 tcgcgtcagc atcgacgccg tcgatgattc ctgaccgccg cgtcgtctgc cacgggaccc
    12541 gatgtggtgg agcccggcaa cgagcctgcg aaggaccggt cgcgcgcacg cgcgggacgt
    12601 cggatcgccg acaaccgggc atccgcgcga cacaacatcc ccgctccgac gtcctccggt
    12661 catgcacccc gatcgtggtg ccaccagagg agcggccatg accctcggtg tccgaagcca
    12721 tcgccagcct cgaccctcgc cgtggtccgg tcccgcggtg tttcagacgt cagccgtggc
    12781 aaccgccatg acccggcgaa cgcccacag
//
LOCUS       DATAIW010000236         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_162383, whole genome shotgun sequence.
ACCESSION   DATAIW010000236 DATAIW010000000
VERSION     DATAIW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162383"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            707..988
                     /locus_tag="VHM48_07515"
     CDS             707..988
                     /locus_tag="VHM48_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755294.1"
                     /translation="MAGRIGDDRDVRAGRSGRRRRPTARPSGACPRTLPYGSWLEVSG
                     HFDDPAAAGCVRGWTPDEADSISPETAAEQVFSCREQFVITAVRPVAAP"
     gene            complement(1011..1964)
                     /locus_tag="VHM48_07520"
     CDS             complement(1011..1964)
                     /locus_tag="VHM48_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX2755295.1"
                     /translation="MSGDGAPARPATPGHVWVDGRIVSAATPVLTAFDRGFQLGDAIF
                     ETLRARAGRPTELPEHVARLHRSAVGLAIVLPDHLERRLAVAIADLLAVERLGGPDGD
                     ASIRITVSRGAFFGRGLLPPDEHPAPTIVVQAWPVPPTPAAHLSDGLHLVPSSVRRDP
                     ENPLAAMKTTSRADYVFARIEARTAGADDALFLTIDGYLSEATSANLFLVRGGASGAH
                     ATGEGDAPELATPSLACAILPGTTRDWILRWGASAGLRPVETWLTTRDLAEADEAFLS
                     SSVAGILPVTRFAGEPIGTGRPGSWTLRARADREAFMRSPG"
     gene            complement(1961..3448)
                     /locus_tag="VHM48_07525"
     CDS             complement(1961..3448)
                     /locus_tag="VHM48_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX2755296.1"
                     /translation="MIHLVGDRSVAPAPSVRLEPLAEIAGARPIDVAEAFRDLPGLAL
                     LESARPGRNARWTFLTADPVAVLTEPAAGGDPFAVARRLLARLAPSAPARHQGGPDAP
                     PFLGGLVGFLAYELADALERLPSPAADDQDLPLLRLALHDWTIAWDRRTGGAWLAGRA
                     LDGDVDRLKRRLAGVHDRIRPGRLVRAAALSPADDRAADGITFISSLDRAAYEAGVEA
                     VRARIAGGDIYQANLTRRLSAPFDGDPWPLYRRLRTGDPSLFSAYLDLGRGAPGHGSL
                     LGRAPARPRAILSASPEPFLAISPRGTVSTDPIKGTRPRGRTRAEDRALARELLASGK
                     DRAENVMIVDVLRNDLGRVCIPGTVRVPRLCRLERTATVQHLVSTVTGRLAPGRDPFD
                     LLRASFPGGSITGAPKIRAMEILRELEPTRRGPYTGALGWIGPDGSMATSILIRTLVA
                     DGSRLSLHVGGGITWQSDPAAEWDETVAKARGPLAAIGGREVGSE"
     gene            3574..>4468
                     /locus_tag="VHM48_07530"
     CDS             3574..>4468
                     /locus_tag="VHM48_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2755297.1"
                     /translation="MRARDLGIVIGRGRPGPANAITDVAGVRVGHATIVRGDGPLVVG
                     EGPVRTGVTMIVPHDGDPWTEPVYAGSHTLNGNGELTGLEWIREAGHLTGPIGITNTH
                     SVGVVRDAIIRNAVRILPFGTPYWALPVVGETWDGLLNDIDGFHVTADHVDEALAAAR
                     GGPVDEGAVGGGTGMVCHEFKGGIGTASRVVGADWGGFTVGVLVQANYGRRDWLRVDG
                     VPVGEAIPIEEIASPYTGTEDDDQKPGPPPGSGSIIVIVATDAPLLPHQCERLAQRAG
                     LGVARMGGTGAHSSGDLFLCFA"
BASE COUNT          624 a         1599 c         1693 g          552 t
ORIGIN      
        1 ccgcgccgga acggcgacga tgcctttcgc tgtcatgctg cgtcgagaga ccatggggat
       61 cgccctcgac gaggtgaccc ctgaccgtca cctgcggcgt cgcggtcgag ccgaccccga
      121 tcgggctgtt cgcagtcacg ctgtcggtcc actgggatgc cgagccaggg tatgccgcgg
      181 gcacctatcg ggaggtcatc gacctcgacg gccagggcgc gctcggcgcc gagcggacgc
      241 tgaccgacag ctccttcccc gcgactcgtc ccgcggtcac gcccggtccg gcgacgacgc
      301 cgatcgccct cgagccgggc agcctggtcg aggtcctcta cccgggggtg acgttctacc
      361 gggaggcgaa ccgcaacgac gggctgtccg acctgccgca cggcgcgcgg ctgtggatcg
      421 tcggcgggcc gacgatcgtc gacggtgagc agtggtatcg cgtccaatgg cagccgaccc
      481 cgacgtacga cgggatcccg gggtggatgc ccgcaaccct cgacggccat cccgtggtcg
      541 cccccgcggc cccacgctgc ccgtccgggg agatcgacgc cgtcggcctc gtcggcctga
      601 tcccggcgga gcgcctcgcc tgccttggtg gccgggacct gacgctcggg ccggtgaccc
      661 tcgcgctgcg gtcgaacggg acggcacagg cgaccggatc accgtcatgg ctggccgaat
      721 cggcgacgat cgcgatgtac gggccggacg gtccggacgg cgtcgacggc ccactgctcg
      781 tccgtccggg gcctgtcctc ggaccctgcc gtacggatcg tggctcgagg tcagcgggca
      841 cttcgacgac ccggcggcgg ccggatgcgt ccgcggctgg accccggacg aggccgactc
      901 gatctcgccg gagaccgccg ccgagcaggt cttcagctgt cgcgagcagt tcgtgatcac
      961 cgccgtccgg ccggtcgccg cgccctgagg actcgctcgt ccgacctcga tcagccgggg
     1021 ctgcgcatga acgcctcgcg gtcggcccgg gcgcggagcg tccacgaccc gggccgcccc
     1081 gtcccgatcg gctcgccggc gaaccgggtc acgggcagga tccccgcgac ggagctcgag
     1141 aggaaggcct cgtcggcctc ggcgaggtcg cgggtggtga gccaggtctc gaccggtcgg
     1201 aggccggccg aggcacccca gcggaggatc cagtcgcggg tcgtgccggg caggatcgcg
     1261 caggcgagcg acggcgtcgc cagctccggt gcgtcgccct cgcccgtggc gtgagcgccg
     1321 ctcgcgccgc cgcgaacgag gaacaggttg gccgacgtcg cctccgagag gtagccgtcg
     1381 atcgtgagga acagggcgtc atcggcgccg gccgtccgtg cctcgatccg ggcgaagacg
     1441 tagtcggcac gggacgtcgt cttcatcgcc gcgagcgggt tctcggggtc ccggcggacc
     1501 gagctcggga cgaggtggag gccgtccgag agatgcgcgg ccggcgtcgg cgggaccggc
     1561 cacgcctgga ccacgatcgt cggcgccgga tgctcgtcgg gcggaagcag gccgcgcccg
     1621 aagaacgccc cacgcgacac ggtgatccgg atactggcgt cgccgtcggg tccgcccagc
     1681 cgctcgacgg cgaggaggtc ggcgatcgcg accgcgagcc ggcgctcgag gtggtcgggc
     1741 aggacgatgg ccagaccgac ggccgagcga tgcaggcgag cgacatgctc gggaagctcg
     1801 gtcggccggc cggcgcgggc gcggagcgtc tcgaagatcg cgtcgcccag ctggaacccg
     1861 cgatcgaacg cggtcaggac cggcgtcgcg gccgacacga tccgcccgtc gacccagacg
     1921 tggccgggcg tcgcgggacg ggccggagcc ccatcgccgc tcattcgctg ccgacctcgc
     1981 gcccgccgat cgccgccagc ggcccgcgcg ccttggctac ggtctcgtcc cactcggcgg
     2041 cgggatcgct ctgccaggtg atcccgcccc cgacgtggag cgacaggcgc gacccgtcgg
     2101 cgacgagggt ccggatgagg atcgaggtcg ccatcgatcc gtccggcccg atccagccga
     2161 gggcgccggt gtacggcccg cgccgagtcg gctcgagctc gcgcaggatc tccatcgccc
     2221 ggatcttggg ggcgccggtg atcgagccgc cggggaacga ggcgcgcagc aggtcgaacg
     2281 ggtcgcggcc cggcgcgaga cggccggtca cggtcgagac gagatgctgg acggtggccg
     2341 tccgctcgag ccggcagaga cgcggcaccc ggaccgtgcc cgggatgcag acgcggccga
     2401 ggtcgttccg gaggacgtcg acgatcatca cgttctctgc ccggtccttg cccgaggcga
     2461 gcagctcccg ggcaagggca cggtcctcgg cgcgggtccg gccgcgcggt cgggtgccct
     2521 tgatcgggtc ggtcgagacg gttccgcgcg gcgagatcgc gaggaacggc tcgggcgacg
     2581 ccgagaggat cgcccggggc cgagccggag cacgaccgag caaggagccg tggcccggcg
     2641 cgccacggcc gaggtccagg tatgccgaga acagtgacgg atcacccgtt cgcagacgcc
     2701 ggtacagcgg ccacgggtcg ccgtcgaacg gtgcgctcag ccggcgggtg aggttggcct
     2761 ggtagatgtc accgccggcg atccgggcgc ggacggcctc gactcccgcc tcgtatgccg
     2821 cccggtcgag cgatgagatg aaggtgatcc catccgccgc cctgtcgtcg gccggcgaga
     2881 gggcggccgc ccggacgagc cggccgggcc ggatccggtc gtggacgccg gcgagtcgcc
     2941 gcttcaggcg atcgacatcg ccatcgaggg cccgcccggc cagccatgcg ccgcccgtcc
     3001 ggcgatccca ggcgatcgtc cagtcatgga gcgcgagccg gagcagcggc aggtcctggt
     3061 cgtcggccgc cggcgatggc aggcgttcga gcgcgtccgc cagctcatag gcgaggaatc
     3121 cgaccaggcc gccgaggaac ggcggcgcat ccggcccacc ctggtgtcgt gccggcgcgc
     3181 tcggagcgag acgggcgagg aggcgacgcg cgaccgcgaa cgggtcgcca ccggccgccg
     3241 gctcggtcag gaccgcaacc gggtcggccg tcaggaacgt ccagcgggca ttgcgtcccg
     3301 gcctggcgct ctcgaggagg gccaggcccg ggaggtcgcg gaatgcctcg gcgacatcga
     3361 tcggtcgcgc gccggcgatc tccgcgagcg gctcgagccg cacggacggg gccggcgcga
     3421 cggaacggtc gcccaccaag tggatcaccc ggcgatgata gggaccgcga gccgaagcgg
     3481 ctccagacgg tccgatcgac gagcggcgga cgggaatacc gaggcaaccc gccggtcgcc
     3541 ggccggcgca gcgcagacgc gggaggcaac cgggtgcgag cgagagacct cgggatcgtc
     3601 atcggtcgcg gccggccggg cccggccaac gcgatcaccg atgtcgccgg cgtgcgcgtc
     3661 gggcatgcca cgatcgtccg cggtgacggg ccgctcgtcg tcggcgaggg tcccgtccgg
     3721 accggcgtca cgatgatcgt cccccacgac ggcgacccct ggaccgagcc ggtctacgcc
     3781 ggcagccaca ccctcaacgg caatggtgag ctgaccgggc tcgaatggat ccgcgaggcc
     3841 ggccacctga ccggcccgat cgggatcacg aacacccact cggtcggggt ggtccgcgac
     3901 gcgatcatcc gcaacgccgt ccggatcctg ccgttcggga cgccgtactg ggcgcttccg
     3961 gtcgtcggcg agacgtggga cgggctgctc aacgacatcg acgggttcca cgtgacggcc
     4021 gatcacgtcg acgaggccct ggccgccgcc cgcggcggac cggtcgacga gggcgcggtc
     4081 ggcggcggaa ccgggatggt ctgccacgag ttcaagggcg gcatcgggac ggcttcccgg
     4141 gttgtcggcg cggattgggg cgggttcacc gtcggcgtcc tcgtccaggc caactacggc
     4201 cggcgcgatt ggctccgggt cgacggcgtg cccgtcggcg aggcgattcc gatcgaggag
     4261 atcgcgagcc cctataccgg gaccgaggac gacgaccaga aaccgggccc gccaccgggt
     4321 tccggctcga tcatcgtcat cgtcgccacc gatgctccgc tcctgccgca ccaatgcgaa
     4381 cggctggccc agcgggctgg cctcggggtc gcccggatgg gcggaaccgg tgcccattcg
     4441 agcggcgacc tgttcctctg cttcgcaa
//
LOCUS       DATAIW010000237         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_214169, whole genome shotgun sequence.
ACCESSION   DATAIW010000237 DATAIW010000000
VERSION     DATAIW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214169"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2443
                     /locus_tag="VHM48_07535"
     CDS             <1..2443
                     /locus_tag="VHM48_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2755298.1"
                     /translation="KTLPNGAANPAYDDVDLNAAADGRVAQRSAFIRTAYQESDEVLT
                     LARSLIGKDPTTFVSSDHGFAPQFLAIDASLPLVQLGLLSRPQTSNCRPATGETIGKA
                     KACWAGGTVQIYLNLAGRDPAGGGLTQVAAGDVNATVAAIKAKYQGLSDPNDWTHDSI
                     AENWKMIDRVYTKAEARYIPNGPNGSTADMSSPTRTGDVVAFAYPPYQFDAETPGTLV
                     AASKFFGQHGYVPDVQVLSANVNMRATFLAGGQSIAKGQVSARTIDLAPTLSYMLGIP
                     EPQMSEGRVLTGAIKGGPAIKPISIVGLSDFHGQLDQSTLAFDGINATVGGAANLATM
                     FDEEFANLPGPGLLLAAGDNVGASPPNSALLQDMPAIDAENAWGLDATSYGNHEFDYG
                     VARLKAQQARAHFPFLATNIVETATGKAPDWVTPSKVFTVNGIKVGVIGAELQNTPEL
                     VAAGNTAGLSFLPEAARIKAESQRLKARGVIVQVVVIHQGTNVGLNPIGNAAGVTWQG
                     PILAIADALQDTTVDAMIVGHTHRISNLMQGHILVTEGFNAGASYSVLQLMVTGGDVT
                     WAGGATRVAKNIGVAARADVKAIVDDANAQTAVLRNQVIGTQLTDITRAPDRLHESEM
                     GNMVADSMRLRYPGIDAAYINSGGLRANLVVTPPSAGELAGQITWGEMFSVLPFGNRS
                     IIETLTGAQLQTAFLNGFTPFCDALFAGGTGRFPQISGLKVQFHCSGTTPVVDGMWKA
                     PNGPSGTLTEIGPTDTLRIVTLDFMYTGGDGYTVLAGGTNVASPGDDMLQVAIDYVTA
                     HSPVDPQVEGRIVGP"
     gene            2780..>3057
                     /locus_tag="VHM48_07540"
     CDS             2780..>3057
                     /locus_tag="VHM48_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755299.1"
                     /translation="MFGSLTASRRGFRVHRVLIALQVVLLLFAMVAPVGTIAAEPSAP
                     PTESPAPSTDPSADPTATPSADPSADPSPAATPDPTATPDPTADPTATP"
BASE COUNT          506 a         1137 c          964 g          450 t
ORIGIN      
        1 gaagacgctc ccgaacggcg ccgcgaaccc cgcctatgac gacgtcgacc tgaacgctgc
       61 ggccgacggc cgcgtcgccc agcgatccgc gttcatccgg acggcctacc aggagtccga
      121 cgaggtcctg accctggccc gatcgctgat cggcaaggat ccgacgacct tcgtctcctc
      181 cgaccacggt ttcgcgccgc agttcctcgc gatcgacgcg agcctgcctc tcgtccagct
      241 gggcctcctg tcgcggccgc agacctccaa ctgccgaccc gccacgggcg agaccatcgg
      301 caaggcgaag gcctgctggg ccggtggcac cgtccagatc tatctcaacc tggccggccg
      361 cgatccggcc ggcggcggcc tgacccaggt ggccgcgggc gacgtcaacg cgaccgtcgc
      421 cgcgatcaag gccaagtacc agggcctgtc cgatcccaac gactggaccc acgactcgat
      481 cgccgagaac tggaagatga tcgatcgggt ctatacgaag gccgaggccc gctacatccc
      541 gaacggtccc aacggaagta cggccgacat gtcgagcccg acccggaccg gcgacgtcgt
      601 cgccttcgcg tacccgccgt accagttcga cgccgagacg ccgggcacgc tggttgccgc
      661 atcgaagttc ttcggtcagc acggctacgt ccccgacgtc caggtcctgt cggccaacgt
      721 gaacatgcgg gcgaccttcc tggccggcgg ccagagcatc gccaagggtc aggtctccgc
      781 tcgcacgatc gacctcgccc cgaccctgtc gtacatgctc gggatccccg agccgcagat
      841 gagcgaaggt cgcgtcctga ccggcgcgat caagggcggt ccggcgatca agccgatctc
      901 gatcgtcggc ctcagcgact tccacggcca gctcgaccag agcacgctcg ccttcgacgg
      961 gatcaacgcg acggtcggtg gagccgccaa cctcgcgacg atgttcgacg aggagttcgc
     1021 gaacctgccc ggcccgggcc tgctcctggc agctggtgac aacgtcgggg catcgccgcc
     1081 gaactcggcg ctcctccagg acatgccggc gatcgacgcc gagaacgcct ggggcctcga
     1141 cgcgacgtcc tatggcaatc acgagttcga ctacggggtc gctcggctga aggcccagca
     1201 ggcgcgtgcc cacttcccgt tcctcgccac caacatcgtc gagaccgcga cgggcaaggc
     1261 gcccgactgg gtgaccccgt cgaaggtctt caccgtcaac ggcatcaagg tcggtgtgat
     1321 cggcgccgag ctccagaaca cgccggagct cgtggccgcc ggcaacaccg ccgggctgtc
     1381 tttcctgccc gaggcggcgc ggatcaaagc cgagtcgcag cgtctgaagg cccgtggcgt
     1441 catcgtccag gtcgtcgtga tccaccaggg cacgaacgtc ggcctgaacc cgatcggcaa
     1501 cgccgccggc gtgacctggc agggcccgat cctcgccatc gcggatgccc tccaggacac
     1561 gaccgtcgac gcgatgatcg tcggccacac ccaccggatc tccaacctga tgcagggcca
     1621 catcctggtc acggaaggat tcaatgcggg cgcgagctac tcggtcctgc agctgatggt
     1681 caccggcggt gatgtcacgt gggccggcgg cgcgacccgc gtcgccaaga acatcggcgt
     1741 ggccgctcgc gcggacgtca aggcgatcgt cgacgacgcc aacgcccaga cggcggtcct
     1801 ccgcaaccag gtcatcggga cccagctcac cgacatcacg cgggcacccg accgactcca
     1861 cgagtcggag atgggcaaca tggtcgcgga ctccatgcgc ctgcgctatc ccggcatcga
     1921 cgccgcgtac atcaactcgg gtggcctgcg agccaacctc gtcgtgaccc cgccatccgc
     1981 cggcgagctg gcgggccaga tcacctgggg cgagatgttc agcgtgctgc cgttcggcaa
     2041 ccgctcgatc atcgagaccc tcaccggcgc ccagctccag acggcgttcc tgaacggctt
     2101 caccccgttc tgcgatgcgc tgttcgccgg cggcaccggc cgcttcccgc agatctccgg
     2161 cctgaaggtc cagttccact gcagcggcac gacaccggtc gtggacggca tgtggaaggc
     2221 accgaacggc ccgagcggaa cgctgacgga gatcggtccg acggacacac tccggatcgt
     2281 caccctcgac ttcatgtaca ccggcggcga tggctacacc gtcctcgccg gcggcacgaa
     2341 cgtcgcctcg ccgggcgacg acatgctcca ggtcgcgatc gactacgtca ccgcccactc
     2401 gcccgtcgac ccgcaagtcg aaggacggat cgtcggcccg taaccaccct cgacccgcac
     2461 tgaacgcgag agccggcccg gcgggccggc tctcgcgatt cccggcgcga cttccgcggc
     2521 tcatggccca ttcggatccc gatggcctac ccgggcggca tccccggccc ggtgcctgtc
     2581 ggcaccggat gttgaggccg ggtgaagttg ggaatggtta cgtcgacatg cgactggtac
     2641 cgtcagcgcg tcccgtatgg gaccgtactt ttcgccggtc gtatggccgg aagtcatgat
     2701 ccgatggcga tcgccatcac cgagcgcgca ccgggctgga gtcaaggggt cgactcgtcc
     2761 gggcagggga gtcatatcga tgttcggttc gctgaccgct tcacggcggg gttttcgtgt
     2821 ccatcgtgtt ctcatcgccc tgcaggtcgt cctcctgctc ttcgcgatgg tcgcgcccgt
     2881 gggcacgatc gcagcggagc cgtcggcgcc accgaccgag agcccggcgc cgtcgaccga
     2941 cccgtcggcc gatcccacgg cgacaccctc ggcggatccg tcagccgatc cctcgcccgc
     3001 ggccacgccc gacccgaccg ccacgccgga tccgaccgcc gatcccacgg cgacccc
//
LOCUS       DATAIW010000238         4893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_244105, whole genome shotgun sequence.
ACCESSION   DATAIW010000238 DATAIW010000000
VERSION     DATAIW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244105"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..424)
                     /gene="rplT"
                     /locus_tag="VHM48_07545"
     CDS             complement(74..424)
                     /gene="rplT"
                     /locus_tag="VHM48_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX2755300.1"
                     /translation="MARVKRGVTSHKRHKRLLNAAEGRMGAKSRLIKASREANLHALA
                     YATRDRKQRKRQMRELWIVRINAAARLNGMTYGAFVAGLKKAEIELDRKTLADVAVRD
                     AGTFTRIVEAVRGA"
     gene            complement(440..637)
                     /gene="rpmI"
                     /locus_tag="VHM48_07550"
     CDS             complement(440..637)
                     /gene="rpmI"
                     /locus_tag="VHM48_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX2755301.1"
                     /translation="MPKIKSHKAAAKRIGVTGSGKFVRVKAWSGHHLEIKSSRRTRRY
                     AGKAVMVGVHAKQVRRLLPYL"
     gene            complement(645..1538)
                     /gene="infC"
                     /locus_tag="VHM48_07555"
     CDS             complement(645..1538)
                     /gene="infC"
                     /locus_tag="VHM48_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX2755302.1"
                     /translation="MLSFRLDWRPGRCAFACRSNHREHVRLKGSAIARDLRVNQMIRI
                     PQVRVVDEEGAQLGVMPTPQALAMAQERGLDLVEVAPMAAPPVVKFLDFGQYKYELTK
                     REKENKRRQRSVTFKEVRLSPKIGTGDFDTKVHRAIEFLEDGDRIKVTVRFRGRELTH
                     PELGRNMLAKFTDRIKEHGVAERTPLLEGKSMHLTVASVHKPKEHEAPTRTRPTDGVA
                     PAAPAPDEPESAAITADKPQPAAPAPAAAAVQEAPVEPAPVAAVAAAVAPKKTAIDAA
                     PAAAARTSDPSGPQAPAATGE"
     gene            complement(1676..2197)
                     /locus_tag="VHM48_07560"
     CDS             complement(1676..2197)
                     /locus_tag="VHM48_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755303.1"
                     /translation="MDEAHAALVDAFVLLLRGLGWVVEVEVSFSIWGERGSIDILAFR
                     PLFGALLIVEIKSVIADSQATLHGLDRKARLARDVIQGRGWDVRHVSRLLVIGATATA
                     RRRVARLAATYDAALPERGTVTRRWLKRPDRPLAGLLFLANASHGSTRKATVARERVR
                     RPKSRSDDSQAGA"
     gene            complement(2460..4373)
                     /gene="thrS"
                     /locus_tag="VHM48_07565"
     CDS             complement(2460..4373)
                     /gene="thrS"
                     /locus_tag="VHM48_07565"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2755304.1"
                     /translation="MTDAKVDSKAKAKDAAPDPTAVATDEADSTELEEALFDAPERGS
                     ADELLDAGAHSDLDAMRHSTAHVMAEAVLDLFRGTQLGIGPAIVDGFYYDFKLDRPLT
                     PADLAAIEERMAASVAADHPFVRRELPPDEGRALFAERNQPFKVEILDDLAAKARSDG
                     TPMPETTLYQHGPFEDLCKGPHVASTARIGPFKLLAISGAYWRGDEKRPALQRIYGTV
                     WQSQEDLDAYLWRRAEAKKRDHRRLGVALDLFSFHDVSPGSAFWHPKGQMIWRTLEGA
                     MRELQSKRGYQEISTPILVDKKLWQQSGHWDLYDANMFRLTSEDHDFALKPMNCPEST
                     FIYKSKVRSYRDLPLRLNEYGRLHRNERSGTLSGLTRVRQFIQDDAHVYVRPDQLADE
                     IEAMLGEVHEAYSWVGLTPRFAFATKPDKAIGDPALWERAEALVKAALDKAGGDYKVK
                     PRDGTFYAPKIDIYIDDALGREWQMATIQADLTMLPERFDLHYIDEHGQPQRPIAIHR
                     AIYGSLERFIGILVEHFAGAFPFWLAPVQAVVIPIADRHIEAAEELAVVLRSRGLRVD
                     VDGSDSRMQNKIRIAQEQKIPYMVVLGDREIGAREGTLRRRGAEKGEPQETLGWDALA
                     DRLAEESRSRSVT"
     gene            complement(4646..4722)
                     /locus_tag="VHM48_07570"
     tRNA            complement(4646..4722)
                     /locus_tag="VHM48_07570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4686..4688),aa:Val,seq:gac)
BASE COUNT          674 a         1678 c         1691 g          850 t
ORIGIN      
        1 ccgacgccga gggaatcctc gggccgatgc gctccggaga gcgcgctcgg acctcggagg
       61 gtggatcgcc agatcaggcg ccgcggactg cttcgacgat ccgggtgaac gtgccggcgt
      121 cgcggaccgc gacatcggcg agggtcttcc ggtcgagctc gatctcggcc ttcttcaagc
      181 cggccacgaa cgcgccgtag gtcatcccgt tgagacgggc cgccgcattg atccgcacga
      241 tccacagttc gcgcatctgg cgcttccgct gcttgcggtc gcgggtggcg taggcgaggg
      301 cgtggaggtt ggcctcgcgc gaggccttga tcaggcgcga cttcgcgccc atccggccct
      361 cggccgcgtt gaggaggcgc ttgtggcgct tgtggctggt gacgccgcgc ttgactcgtg
      421 ccatgaggtc gatccgctcc tagaggtacg gcagcaggcg tcggacctgc ttcgcgtgga
      481 cgccgaccat gacggccttg ccggcatagc gtcgggttcg gcgggacgac ttgatctcga
      541 ggtggtggcc gctccaggcc ttcacccgga cgaacttgcc gctgccggtc acgccgatcc
      601 gcttcgcggc tgccttgtgg ctcttgatct ttggcaccgt ggcctcactc tcctgtggct
      661 gccggtgcct gcggccccga cgggtcggac gttcttgcgg cggccgcggg ggccgcatcg
      721 attgccgtct tcttcggtgc gacggccgcc gccacggcgg ccaccggcgc cggttcgacc
      781 ggagcctcct gcacggcggc tgcggccggt gccggcgcgg ccggctgcgg tttgtcggcc
      841 gtgatcgcgg cggactccgg ctcgtccggc gccggagcgg ccggagccac gccgtcggtc
      901 ggacgggtcc tggtcggcgc ctcgtgctcc ttgggcttgt ggaccgacgc gaccgtgagg
      961 tgcatcgact tgccttcgag caacggagtg cgttccgcga cgccgtgttc cttgatccgg
     1021 tccgtgaact tcgcgagcat gttccgaccg agctccgggt gggtcagctc ccggccgcgg
     1081 aagcggacgg tcaccttgat ccggtcgcca tcttcgagaa actcgatcgc ccgatggacc
     1141 tttgtgtcga agtcgcctgt gccgatcttc ggcgagaggc ggacttcctt gaaggtcacc
     1201 gagcgctgcc ggcgcttgtt ctccttctcg cgcttggtca gctcgtactt gtactgaccg
     1261 aagtcgagga acttcacgac cggcggggcc gccatcgggg cgacttcgac gaggtccaac
     1321 cctcgctcct gcgccatggc gagagcctgg ggcgtgggca tcacgcccag ctgtgctccc
     1381 tcttcatcga cgacgcggac ctgtgggatc cggatcatct ggttgacgcg caggtctcgt
     1441 gctattgctg accccttcaa acgaacgtgt tcccgatggt tcgaccggca ggcgaacgca
     1501 cagcgcccgg ggcgccagtc gagccggaac gatagcacag gccccgaatc cggggcaatc
     1561 ggtcagggcc ccgtggcggc ggccgactgt gccgcgcccg tcccgtcgtt tcgctcattc
     1621 gacagccagg ttcgcccaat ccacccgccg cccggctgcc gtgctgtcac agcgatcatg
     1681 cgccggcctg gctgtcgtct gagcgcgatt tcggcctccg aacccgctcc ctggcgacgg
     1741 tcgccttcct tgtgctgcca tggctggcat ttgcgagaaa cagcagcccg gcgagcggcc
     1801 gatcaggcct cttcagccag cgccgagtca ccgtccctcg ttcgggtagt gccgcgtcgt
     1861 acgttgcagc caaccgggcc acgcgtcgcc gcgcggtcgc cgtcgcgccg atgacgagca
     1921 gccggctgac atggcgcacg tcccatccgc ggccctggat cacgtcgcgc gcgagtcgcg
     1981 ctttgcgatc caacccgtga agcgtcgcct gggagtcagc gatgaccgac ttgatctcga
     2041 cgatgagcag ggcgccgaac agtggacgga aggcgagaat gtcgatcgac ccgcgctcgc
     2101 cccagatcga gaacgaaacc tcgacctcga cgacccaacc aagccctcga aggagcagca
     2161 cgaacgcatc gaccagtgcg gcatgcgcct cgtccaccag ccgatcgagt cgctcgcctc
     2221 gccatcgaag gtagaggtcg acgtccgccc cgagcgcagc cgccgccctg acgagcgacc
     2281 cgaccgtcac gccgtccagc tcgccccggt cgatcgccgc gaccaacgaa cgcgagacgc
     2341 cggcgcgcgc cgagacgtcc tcctggcgtc gttcctgacg gatccgaagc gcccggaagt
     2401 gccgtccaaa tcggagagcg tccacgatcc catcgtgacg ccggccgctt cacgcccgct
     2461 tacgtgaccg agcggctgcg cgactcctcg gccagccggt cggcgagggc atcccagccg
     2521 agggtctcct ggggctcgcc cttctcggcg ccgcgccggc ggagcgtacc ctcgcgggcc
     2581 ccgatctcgc ggtcgccgag gacgaccatg tacgggatct tctgctcctg ggcgatccgg
     2641 atcttgttct gcatccggct gtcggagccg tcgacgtcga cccgcaggcc gcgcgaccgg
     2701 aggaccacgg cgagctcctc ggccgcctcg atgtggcggt ccgcgatcgg gatcacgacg
     2761 gcctgcaccg gtgcgagcca gaacggaaac gcgccggcga aatgctcgac gaggatcccg
     2821 atgaagcgct cgagcgagcc gtagatcgcc cggtggatcg cgatcggtcg ctggggctgg
     2881 ccgtgctcgt cgatgtagtg gagatcgaac cgttccggga gcatcgtgag gtcggcctgg
     2941 atcgtcgcca tctgccactc gcggccgagg gcatcgtcga tgtagatgtc gatcttgggc
     3001 gcgtagaacg tcccgtccct gggcttgacc ttgtagtcgc caccggcctt gtcgagggcg
     3061 gccttcacga gcgcctcggc ccgctcccag agcgcggggt caccgatcgc cttgtccggc
     3121 ttcgtcgcga acgcgaaccg cggggtcaag ccgacccacg agtacgcctc gtggacctcg
     3181 ccgagcatcg cctcgatctc gtcggccagc tggtccggtc ggacgtagac gtgggcgtcg
     3241 tcctggatga actggcggac gcgggtcagc cccgacagcg tgcccgaccg ctcgttgcgg
     3301 tggagcctgc cgtattcgtt gaggcgcagg ggcaggtcgc ggtatgagcg gaccttcgac
     3361 ttgtagatga aggtcgactc ggggcagttc atcggcttga gggcgaagtc gtggtcctcc
     3421 gacgtgagcc ggaacatgtt cgcgtcgtag aggtcccagt ggcccgactg ctgccagagc
     3481 ttcttgtcga cgaggatcgg cgtgctgatc tcctggtagc cgcgcttcga ctggagctcg
     3541 cgcatcgccc cctcgagggt gcgccagatc atctgaccct tcgggtgcca gaaggccgaa
     3601 ccgggactga cgtcatggaa gctgaagagg tcgagggcga cgccgagccg gcgatggtcg
     3661 cgcttcttcg cctcggcccg ccgccagagg taggcgtcga ggtcttcctg gctctgccag
     3721 accgtcccgt agatccgctg cagggcgggc cgcttctcgt ccccgcgcca atacgcgccg
     3781 gagatcgcga ggagcttgaa tgggccgatc ctggcggtgc tcgcgacgtg cggccccttg
     3841 cacagatcct cgaacgggcc gtgctggtac agggtcgtct cgggcatcgg tgtgccgtcc
     3901 gacctggcct ttgccgcgag gtcgtcgagg atctcgacct tgaacggctg gttgcgctcg
     3961 gcgaagaggg ccctcccctc gtcgggcggc agctcccggc ggacgaaggg atggtcggcg
     4021 gcgaccgagg cggccatccg ctcctcgatc gcagccaggt cggccggcgt aagcgggcgg
     4081 tcgagcttga agtcgtaata gaaaccgtcg acgatcgccg gaccgatgcc gagctgggtg
     4141 ccccggaaca ggtcgaggac ggcctcggcc atgacgtggg ccgtggagtg gcgcatggcg
     4201 tcgaggtcac tgtgggcgcc ggcgtcgagg agctcgtcgg ccgagccgcg ctcgggcgcg
     4261 tcgaacaggg cttcctcgag ctcagtgctg tcggcctcgt cggtcgcaac cgcggtcggg
     4321 tcgggggcgg cgtccttcgc cttggccttg gagtcaacct tcgcgtctgt catggctcgt
     4381 ccctccggga acaacaaaac ctctcgcctg atcgacagga cgagaggttc actcccgcgg
     4441 tcccacccgc cttcgcgtcg gtcgagccag tttcccggtt cgccgtccgc gctcttgtcg
     4501 ccgcgctaac gggcggatcc cggaccggtt cgccggtcgc tcacgggtgg tgcccggttc
     4561 gtcggtcgcc accgggctcg cagctcgtcc cggctctctg gaggcgacgg cgatgggtct
     4621 gtcccgatcg tcgcgttcgt tgatgtggtg ggcgacactg gactcgaacc agtgacctct
     4681 tgcatgtcaa gcaagtgctc taaccagctg agctagccgc ccgggcggct ccggcgtcgg
     4741 accctggctg ggggccggcc gacggaacct caccatcatg cttcggtcgt ctcgtcgctc
     4801 ggccccagtt gaatgcgagg cgttccagtc ctcacccggg gctccgccgc gggacggcga
     4861 ggcgcgatgg ctgcggcgca ttgtagccga ggc
//
LOCUS       DATAIW010000239         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_102758, whole genome shotgun sequence.
ACCESSION   DATAIW010000239 DATAIW010000000
VERSION     DATAIW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102758"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /gene="upp"
                     /locus_tag="VHM48_07575"
     CDS             complement(<1..415)
                     /gene="upp"
                     /locus_tag="VHM48_07575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX2755305.1"
                     /translation="MTPPTLHVSSHPAILHKLAILRDEKTEPKKFREVVRELSWLLGY
                     EALADVKVRPVTVRTPIEEMEGAELADRIGLVPILRAGLGMVDAMLELMPTAEVWHLG
                     LFRDERTLQPVEYYNKLPDSASVDLCLILDPMLATG"
     gene            714..1925
                     /locus_tag="VHM48_07580"
     CDS             714..1925
                     /locus_tag="VHM48_07580"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX2755306.1"
                     /translation="MTTIDGPVGSTARTTAATRGAERAPRSARAAPARPTRRTAPILV
                     RQMRGGVEESVHRGDVVEVDVEGALIRGLGDPDRVANLRSCVKPFGVVALIEAGGIEA
                     FDLEPAEIALMASSHSGEDLHVRTLQAMYRRAGVSQGLIATGSEGMPLDALTAARLAR
                     DGEKASPVRHMCSGQHSVFLLLSRLKGWDPERYWLDDHPAQVAYRAVVARAFRTTPGR
                     LRTAIDGCGVATYAFPLREIARAYALLADPSAVTTADTRSSLAPALTRVRDAMLAHPE
                     MIGGTRDRLDTSLMKAAPGRVVSKGGMEALRGLAILGGARPGAGGAGRGNETTAAASG
                     IALKIEDGDGHERGTWAATVEVLAQAGVLDGQALRMLARYHRPADLDPHGRTGAEAIP
                     DFELAPLGELL"
     gene            2026..>2365
                     /locus_tag="VHM48_07585"
     CDS             2026..>2365
                     /locus_tag="VHM48_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2755307.1"
                     /translation="MAPNADPWRTLGLAPGATQDEIRRAYRRLAKVHHPDAAGDAELP
                     RFLAIQAAYEQLAGPGRVRRVGARPPGRPTDPGPATDPREPWRADPNRARASGRADGR
                     RTPGARPTGPG"
BASE COUNT          318 a          861 c          863 g          323 t
ORIGIN      
        1 cgcctgtcgc gagcatcggg tcgaggatca ggcagaggtc gaccgacgcc gagtccggca
       61 gcttgttgta gtactcgacc ggctggagcg tccgctcgtc gcggaacagc ccgaggtgcc
      121 agacttcggc ggtcggcatg agctcgagca tcgcgtcgac catcccgagc ccggcccgca
      181 ggatcgggac gaggccgatc cggtcggcga gctccgcgcc ctccatctcc tcgatcggcg
      241 tccggacggt caccggccgg accttgacgt cggcgagggc ctcgtacccg agcagccacg
      301 acagctcgcg cacgacctcg cggaacttct tgggctccgt cttctcgtcg cgcaggatcg
      361 cgagcttgtg gaggatggcg ggatggctcg agacatggag cgtgggcggg gtcaagacgg
      421 gcctcccggc agtgggcgtg ccgccgcggc gacgacggga tcgacggtcc ccgaaatcgt
      481 agcaaggccg acgggaccgg ggcggctcat cgaacgcgct tccgaccgct tcggcgggat
      541 cgcgacgccg gcgagccttc aaccgtgtac cgtccgtcgc ggctggttcg atccggatgg
      601 ctttctcgag tgcccgcgag atgtcgttcg tggtcgatcc ggccatttgg tcgcccggac
      661 ggacgcggat cgatggtcgc gacgcgaccg cggcgcgcta tcctcccggg atcgtgacga
      721 ccatcgacgg cccggtaggc tcgaccgccc gcaccaccgc agcgacgcgc ggcgccgagc
      781 gtgccccccg gtctgcccga gccgcgcccg cccggccgac ccgccgaacg gccccgatcc
      841 tggtccgcca gatgcgcggc ggcgtcgagg agagcgtgca ccgcggcgat gtcgtcgagg
      901 tcgacgtcga gggcgccctg atccgcgggc tcggcgaccc ggaccgcgtg gcgaacctgc
      961 gcagctgcgt caagccgttc ggggtcgtcg ccctgatcga ggccggcggg atcgaggcgt
     1021 tcgacctcga gccagccgag attgcgctca tggccagctc gcattcgggc gaggacctcc
     1081 acgtcaggac tctccaggcg atgtaccgga gagccggcgt cagccagggg ctgatcgcga
     1141 ccggttcgga gggcatgccg ctcgacgccc tgaccgccgc ccgcctcgcc cgcgacggcg
     1201 agaaggccag cccggtccgt cacatgtgct cgggccagca ctcggtgttc ctgctcctgt
     1261 cgcgtctcaa gggttgggac cccgagcgct attggctcga tgaccacccg gcccaggtcg
     1321 cgtatcgggc ggtggtcgcc cgggcgttcc ggacgacgcc cggtcggctc cggaccgcga
     1381 tcgatggttg cggcgtcgcc acctatgcct tcccgctgcg cgagatcgcc agggcctacg
     1441 cgctcctggc cgacccgtca gcggtcacga ctgccgacac acggtcttcc ctcgcgcccg
     1501 ccctgacccg tgttcgcgac gcgatgctcg cccatcccga gatgatcggc gggacccgcg
     1561 accggctcga cacctcgctg atgaaggccg cgcccggtcg cgtcgtcagc aagggcggga
     1621 tggaagcgct gcgcggcctt gcgatcctcg gtggggcccg gcctggtgcc ggtggtgccg
     1681 gccgcggcaa cgagacgact gccgcagcga gcggcatcgc gctcaagatc gaggacggcg
     1741 acgggcacga gcgcggaacc tgggcggcca cggtcgaggt tctcgcccag gcaggtgtgc
     1801 tcgacggtca ggccctccgg atgctcgccc gctatcaccg gccggccgat ctcgatccac
     1861 acggccggac cggggccgag gcgatcccgg acttcgagct ggctccgctc ggcgagttgc
     1921 tctagccacg agcggatcga tgggggccgg aggcctgccg ccgggcggga gtccggtccc
     1981 actcagccct cgacgcattc acgcggctca agcatgcttc ggccgatggc accgaacgcg
     2041 gacccgtggc ggaccctggg actggcgcct ggcgcgaccc aggacgagat ccggcgcgcg
     2101 tatcggcggc ttgccaaggt ccatcatccc gacgcagccg gggacgccga gctgccgcgc
     2161 tttctcgcga tccaggcggc gtatgagcag ctcgccgggc cggggcgcgt ccgacgggtc
     2221 ggcgctcggc cgcccggccg cccgacggat ccgggcccgg cgaccgatcc gcgggaaccg
     2281 tggcgggcgg acccgaaccg ggcgcgggcc tcgggccggg ccgacggccg acggaccccc
     2341 ggcgcgcgcc cgacgggtcc aggag
//
LOCUS       DATAIW010000240         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_274054, whole genome shotgun sequence.
ACCESSION   DATAIW010000240 DATAIW010000000
VERSION     DATAIW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274054"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VHM48_07590"
     CDS             <1..829
                     /locus_tag="VHM48_07590"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEX2755308.1"
                     /translation="GLIGWPKTSGSRGIHVNVRIERRWTFPEVRRAALALARDVERRV
                     PDLATSRWWKEERHGVFIDYNQNAKDRTVASSYSVRPRPDARVSTPLTWDEVPDVDPA
                     AFTVRTVPGRFARLGDPHAAIDEHAGSLDALLELSARHEAQGLGDAPWPPNYAKTLDE
                     PPRVAPSKRRMPSKPLIEIGRAATQAEAMAGLARWKERHPDVWPHLAPADMLVDAMRG
                     RSTTWTRIRLNLEHVPPDQRPAQEALDPDYDPWAGVDWPDRAGQPERAPRKKPSAGD"
     gene            complement(922..1605)
                     /locus_tag="VHM48_07595"
     CDS             complement(922..1605)
                     /locus_tag="VHM48_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2755309.1"
                     /translation="MELYSTDVNDVKSPTGSLRAERAAVTRRRITGAARTLFATRGYA
                     ATTLSQIAIAAGVAVQTVYAVFGSKAGILRALREGLVNDPAADAAFGAAVAETSPSRA
                     LELFARSIRLRWETGHDLVAIHADAASADPMIRDEVDRVLAARRRGIAELAQSLVQHE
                     AALRDAGRPTAIIDALTLPELYAELVDVHGWTADAYEAWLATALRRLAIETSDDDPGG
                     VRGRPSWST"
     gene            1656..2087
                     /locus_tag="VHM48_07600"
     CDS             1656..2087
                     /locus_tag="VHM48_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2755310.1"
                     /translation="MSIETNIAATRRLIQDGFTGGDLAVCDALVAPDCLEHQRGLKPG
                     IEGTKDTIRTLHSWFSDFELTIVDLVASDDTVWIRNRATGVNTGSVLGRPATGRPFDI
                     TVIDVIRFADDKMVEHWGVPDQLGLLIQLGLFGRPEPAAVA"
     gene            2654..3169
                     /locus_tag="VHM48_07605"
     CDS             2654..3169
                     /locus_tag="VHM48_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2755311.1"
                     /translation="METIGARGEADVFVGLVERHLDPAYRLARAILLADQEAEDAVQD
                     ACLAAWRERRSLRDHARFDAWFDRILINRCRDRLRVRQRQRVRAIALEGQTQWRHTMD
                     VGAADSELDSAVDDLDPDHRIVVLLRFWQDLRLEDIAERLDIPLGTVKSRLHYALRAI
                     RSRLEATDGRA"
     gene            3156..>3936
                     /locus_tag="VHM48_07610"
     CDS             3156..>3936
                     /locus_tag="VHM48_07610"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755312.1"
                     /translation="MDEREFEARLGARLHRRFDPAEPTEALGGRVLDAMRAEPGPTRT
                     LRSIGPLAAAAVIVVAVVAVALQYGIPGPVATPSPVPSLSPATAVPGTTIVYQVQPAD
                     GMMPGAAGLKAALQVLEKRLSATGAVGSSVQVRGTDEIVVDLPGIQDATDFRKLLGQT
                     GNLRFVPLPPETYGSQSSPGPQEAVVGKPLPVDEVPLFGGESISQAYPTTGSANPTGS
                     ADVPALGFRLDSKAATMFADYTSKSIGQFFAIVLDGVVITAP"
BASE COUNT          596 a         1429 c         1357 g          554 t
ORIGIN      
        1 gggcctcatc gggtggccga agacgtcggg cagccgcggc atccacgtca acgtccggat
       61 cgagcgccgg tggaccttcc ccgaggttcg gcgggccgcg ttggcgctgg cgcgcgacgt
      121 cgagcgccgc gtgccggatc ttgcgacaag ccgctggtgg aaggaggagc gccacggggt
      181 cttcatcgac tacaaccaga acgccaagga ccggaccgtc gcgagctcct actccgtgcg
      241 gccgcggccc gacgccaggg tgtcgactcc gctcacctgg gacgaggtcc cggacgtgga
      301 tcccgcggcc ttcaccgttc ggaccgtccc cgggcggttc gcacgcctgg gcgacccgca
      361 cgccgcgatc gacgagcacg ccgggagcct tgacgccctc ctcgagctgt ccgcgcgcca
      421 cgaggcgcag ggcctgggcg acgcaccgtg gccgccgaac tacgcgaaga cgctcgacga
      481 gccaccccgc gtcgcgccct cgaaacggcg gatgccatcg aagccactga tcgagatcgg
      541 gcgcgccgcg acccaggcgg aggcgatggc cggcctcgca cgctggaagg agcgccaccc
      601 ggacgtctgg ccgcacctcg caccggcgga catgctcgtg gacgccatgc gcggccgatc
      661 caccacgtgg acgcggatcc gcctgaacct ggagcacgtc ccgcccgacc agcggccggc
      721 ccaggaggcc ctcgatcccg actacgaccc atgggcgggg gtggactggc cggatcgcgc
      781 cggccaacca gagcgggcac cgcgcaagaa gccgtccgcc ggcgactgac ggcatccagg
      841 gaccctacaa cacggaaatc catcggtgga gccggtcgac gtcgtcgttc aggccggcga
      901 cgtaaccgtc cagggacatc gtcaggtcga ccatgacggt cgtccccgga cgcctcccgg
      961 gtcgtcgtca ctcgtctcga tggcgagccg gcggagggcg gtcgccagcc aggcctcgta
     1021 tgcgtccgcc gtccatccgt ggacgtcgac caactctgca tacaattccg gcagggtcag
     1081 cgcatcgatg atggccgtgg gccgcccagc gtcgcggagc gcggcctcat gctggaccaa
     1141 cgattgggcc agctcagcga tcccacgccg acgggccgcc agcaccctgt cgacttcgtc
     1201 gcggatcatc gggtcggcgg aggccgcatc ggcgtggatg gccacaaggt cgtggccggt
     1261 ctcccagcga agtcggatcg agcgcgcgaa caactcgagc gcccgcgacg ggctcgtctc
     1321 agcgaccgca gcgccgaacg ccgcgtcggc ggccggatcg ttgaccaggc cctcgcggag
     1381 cgcccgaagg atgccggcct tcgaaccgaa aacagcgtag acggtctgga cggcgacgcc
     1441 ggccgcgatc gcgatctggc tcaacgtcgt cgcggcgtat ccccgggtcg cgaacagcgt
     1501 gcgggcagct cccgtgatcc gccggcgggt gaccgccgcc cgttcggccc tgaggcttcc
     1561 ggtcgggctc ttgacgtcgt tcacgtcggt agagtataac tccatgtcaa tagagtatta
     1621 ctctattgag cgcctgcacc ggaaggaaca ctgccatgtc tatcgagacg aacatcgctg
     1681 cgacccgccg cctgatccaa gacgggttca cgggcggcga cctcgctgtc tgcgacgcgc
     1741 tggtcgctcc ggactgcctc gagcaccagc gcgggctcaa gcccgggatc gagggtacaa
     1801 aggacacgat ccggaccctc cattcgtggt tcagcgactt cgaattgacg atcgtcgacc
     1861 tcgtcgccag cgacgacacc gtctggatcc gcaaccgggc gaccggcgtg aacaccggta
     1921 gcgtcctcgg ccggccggcg acgggccggc cattcgacat cacggtcatc gatgtcatac
     1981 ggttcgctga cgacaagatg gtcgagcact ggggggtccc ggatcagctc ggattgctga
     2041 tccagcttgg gctgttcggg cggccggagc ctgccgcggt cgcctgactc ggtcggccgg
     2101 gaccggggcg tcgggccgcc tggctccgcg ggtcgtccac tcgaaccggc gcgggacccg
     2161 ctcccaggtc tcccgatgcg ggcgtcaatc gcgccgcctt ggaggtcgac gacgacgatt
     2221 ccgtcccagc cgagcgctcc cgggagcagg tcggtcacga ccgccttcta gagcgatgcc
     2281 gaatggtcgg ccagtcatat cggaggggcc tgccgcgcgg ttggcccaac cctccttcga
     2341 tccagtgatc ccgactgcga cccgcagcca ggcgggcaag ggcgacggcg cgggtcagga
     2401 acgaccgatc ccgggtggct cgcatcacga cctggtgacg cgcccgccga ccaggttgtt
     2461 ccgccccgcg gctcgagccg gcgggacctg cggccagaga ccgcgactcc gaccgtgggg
     2521 tcttcggggc ggctctcatt gcggcagcgc cccctgcagt cccgcgacga cggatgaacc
     2581 tttcggaacg ccccaatcgt cagatggggc ggacgcggcg cgggcccaac tgcgcagcag
     2641 ctcgggagac ctcgtggaga ccatcggggc tcgcggagag gcggacgtct tcgtcggcct
     2701 ggtcgaacgc cacctcgatc ccgcctaccg gctcgcccgg gcgatcctgc tcgccgacca
     2761 ggaggccgag gacgccgtcc aggacgcgtg cctcgccgcc tggcgcgagc gacgctcgct
     2821 gcgcgaccac gcccgcttcg acgcctggtt cgaccgtatc ctcatcaacc gctgccggga
     2881 tcgcctccgc gtgcggcagc gccagcgggt tcgagccatc gcgctcgagg gccagacgca
     2941 gtggcgccac acgatggatg tgggcgccgc cgactcggag ctcgacagcg ccgtcgacga
     3001 cctcgacccc gaccaccgga tcgtcgtgct cctgcgcttc tggcaggacc tgcggctgga
     3061 ggatattgcc gaacggctgg acatcccgct ggggaccgtg aagtcgcgcc tgcactacgc
     3121 gctgcgcgcg atccgaagcc gcctggaggc caccgatgga cgagcgtgag ttcgaggcgc
     3181 gcctgggcgc gcgcctccac cgccggttcg acccggcgga gccgaccgag gcactcggcg
     3241 gacgagtcct cgacgccatg cgcgccgagc ccggtcccac ccggaccctg cggtcgatcg
     3301 ggccgctcgc ggcggccgcg gtcatcgtcg tcgccgtcgt cgccgtcgcc ctgcagtacg
     3361 gtatcccggg accggtcgcg acgccgtccc ccgtgccctc gctgtcaccc gccaccgccg
     3421 tcccgggcac gacgatcgtc taccaggtcc agccggccga tgggatgatg cccggcgccg
     3481 cgggcctgaa ggccgccctc caggtcctgg agaagcggct ctcggcgacg ggcgccgtcg
     3541 ggtccagcgt gcaggtgcgg ggcaccgacg agatcgtcgt cgacctcccg gggatccagg
     3601 atgcgactga cttccggaag ttgctcgggc agactggcaa cctgaggttc gtgccgctcc
     3661 caccggagac gtatgggtcg cagtcttcgc cgggaccgca ggaggcggtg gtgggcaagc
     3721 cgctgcccgt cgacgaggtg ccactcttcg gcggcgagtc gatctcgcag gcgtatccga
     3781 cgacgggctc cgcgaacccg acgggctccg cggacgttcc tgccctcggc ttccggctcg
     3841 actccaaggc cgcgacgatg ttcgccgact acacgagcaa gagcatcggc cagttcttcg
     3901 cgatcgtcct tgacggcgtc gtgatcaccg ccccga
//
LOCUS       DATAIW010000241         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_102799, whole genome shotgun sequence.
ACCESSION   DATAIW010000241 DATAIW010000000
VERSION     DATAIW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102799"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VHM48_07615"
     CDS             complement(<1..925)
                     /locus_tag="VHM48_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX2755313.1"
                     /translation="MRATATDDRLAARPAASRARPFAWARGRVAALALGGFVALAVAL
                     VLGVGLGTVAIAPGETLGILVHRLLGLDVGGTWSPATETIVWDLRLPRVLTAMTVGAG
                     LAVAGTTFQGLLRNPLADPYVLGTASGAALGAAIAVVIPFRATILGFGLIHALAFVGA
                     LAAVFTVYRLSRINGLSPLTSLLLTGYAVGSLLAAGLAMAMYLSGAALRQIFAYLLGG
                     FDTAGWSQLGVTLPLVAVGSGLILLRARSLDGLLLGEDAALHLGIDVRKERAILLALA
                     SLVTAAAVAVSGLIGFVGLVVPHVVRLAVGPG"
     gene            complement(974..2077)
                     /locus_tag="VHM48_07620"
     CDS             complement(974..2077)
                     /locus_tag="VHM48_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2755314.1"
                     /translation="MPAFRSSSHRPTGRRGPRLPFVLLAVLAVLVAACGGAATARPST
                     PPDATGALLPPATATAPASAYPVPSLALAFPMTLTDDEGTAVELKAEPKKIVSLTPAT
                     TETLFALGVGDRIVGKVEDITPYPPGANAIPVVAKFGEVDVEKIVALGAEVVIAGGNS
                     FNPPPKIAQLRKLGVPVLVVYAPDIAGVLKDVELTGDVVGRSAEARDLTASMQAAFDQ
                     VQAATADFPKPRVFYELDASQKIYTAADSSFLAEMITLAGGEPITTGSTTNFEIPLEK
                     LVTADPELILLGDAAYGTTAATVKARNGWAGMSAVKAGAIVAVDDVLISRPGPRLVNG
                     LRALALAIHPDAAVPAPPAPAASASAASASPAT"
     regulatory      complement(2201..2340)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2343..2510
                     /locus_tag="VHM48_07625"
     CDS             2343..2510
                     /locus_tag="VHM48_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755315.1"
                     /translation="MDGLAMSADGTRLAVVRRGETSTTIDIHVRRDDAWASVRTVILP
                     GVDSASVAWLD"
BASE COUNT          429 a          913 c         1035 g          331 t
ORIGIN      
        1 caccagggcc gacggccagc cggacgacat gcggcacgac gagcccgacg aagccgatca
       61 gcccgctgac cgcgaccgca gcggcggtca cgagggacgc gagcgcgagc aggatcgccc
      121 gctccttgcg gacgtcgatc ccgagatgga gcgcggcatc ttctccgagc aggaggccgt
      181 cgagcgaccg cgcgcgcagg aggatcaggc ccgagccgac cgcgacgagg ggcagtgtca
      241 ccccgagctg cgaccagccc gcggtgtcga agccgccgag gagataggcg aagatctggc
      301 ggagggcggc acccgagagg tacatcgcca tcgccaggcc ggcggccagg agggacccca
      361 ccgcatatcc cgtcaacaag aggctcgtca gcggcgagag cccgttgatc cgcgacaacc
      421 ggtagacagt gaagaccgcg gccagcgcgc cgacgaacgc gagggcgtgg atcaacccga
      481 acccgaggat cgtcgcccgg aagggaatga cgaccgcgat cgcggctccg agcgcggctc
      541 cgctcgcggt gcccagcacg tagggatcgg cgagggggtt gcggagaaga ccctggaacg
      601 tggtcccggc gacggccagc cccgccccga cggtcatggc ggtcagcacc cgtggcagcc
      661 gcaggtccca gacgatcgtc tcggtcgcgg gcgaccacgt cccgccgaca tcgagcccaa
      721 ggagccgatg gacaaggatc ccgagcgttt cgccgggcgc gatcgccacg gtcccgaggc
      781 caaccccgag gacgagggcg acggccagcg cgacgaaacc gccgagcgcg agggcggcca
      841 cccggccccg agcccaggcg aaggggcggg cgcgcgacgc ggccgggcgc gccgccagcc
      901 ggtcgtcggt cgcggtggcc ctcatcgccg gtcctcggcg gatcgcgcgg tcatcgactc
      961 gtccgtcctc ccctcatgtc gcgggcgagg ccgacgcggc ggaggcagat gccgccggag
     1021 ccggcggtgc cgggacggcc gcgtccgggt ggatggcgag ggccagggcg cgcaggccgt
     1081 tgacgaggcg cggcccgggc cggctgatca ggacgtcgtc gacggccacg atggcgccgg
     1141 ccttgaccgc gctcatcccg gcccagccgt tgcgcgcctt gaccgtcgcc gcggtcgttc
     1201 cgtacgcggc atcgccgagc aggatgagct ccggatcggc agtcacgagc ttctcgagcg
     1261 ggatctcgaa gttcgtcgtg ctgccggtcg tgatcggctc gccgccggcg agagtgatca
     1321 tctcggcgag gaacgagctg tcggccgcgg tgtagatctt ctgcgacgcg tcgagctcgt
     1381 agaagacgcg cggcttcggg aagtccgccg tcgccgcctg gacctggtcg aaggcggcct
     1441 gcatcgaggc ggtcaggtcg cgcgcctcgg ccgatcggcc gaccacatca ccggtcaact
     1501 cgacgtcctt gagcacgccg gcgatgtccg gggcgtagac gacgaggacg ggcacgccga
     1561 gcttgcggag ctgggcgatc ttgggcggcg ggttgaagct gttgccgccc gcgatcacga
     1621 cttcggcgcc aagggcgacg atcttctcga cgtcgacctc gccgaacttc gcgacgacgg
     1681 ggatcgcgtt cgcgcccggc ggatacggcg tgatgtcctc gaccttgccg acgatccggt
     1741 cgcccacgcc gagcgcgaag agggtctcgg tcgtcgccgg ggtcagcgag acgatcttct
     1801 tcggctcggc cttcagctcg acggccgtgc cctcgtcgtc ggtcagcgtc atcgggaagg
     1861 cgagggcaag cgagggcacc ggatacgcgc tggccggcgc cgtggcggtc gcgggcggga
     1921 gcagcgcgcc ggtcgcatcg ggcggagtcg acggccgcgc cgtggcggcg ccgccgcacg
     1981 cggcaacgag gacggccagg accgcgagaa ggacgaaggg gaggcgaggg ccgcggcggc
     2041 cggtggggcg gtggctggac gagcggaagg cgggcacgtc gggagctcca ggtcgaagga
     2101 aggagcgggg gccggacacg gcgaacccct cgcttcggac aggaaggagg ggttgattca
     2161 gccggacgtg cgcatcgttg cctgcacctt tctctcgaag gtagagcatc gaccgcgggc
     2221 ccggcgaccg gacttccacc tcgccatccg acgggtggtc accgtagcgg gactgtgccg
     2281 gattctcacc ggcttcgcga ccccgcggca aatcgaggta gcccgaggat gctaccacgg
     2341 cggtggacgg gctcgcgatg agcgccgacg ggacgcgcct ggcggtcgtt cggcgcggcg
     2401 agacgagcac gacgatcgac atccacgtcc gccgcgacga cgcgtgggcg agcgtccgga
     2461 cggtgatctt gcccggcgtc gactcggcgt cggttgcctg gctcgactga ccaggtccgg
     2521 gcaacgagaa gggcgtcgac tcgcggtcga cgccctcgtc gggtgaagcg ccggcgcgac
     2581 cggcgcgcgc tcaggccggg acggcagcct gggctgagaa gatcgattcc tgcgcggatt
     2641 caggcagcat cacgccgacg atcttctgga gtgtcaggac gtggacgcac gtttgccaac
     2701 tgtcgaag
//
LOCUS       DATAIW010000242         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_184756, whole genome shotgun sequence.
ACCESSION   DATAIW010000242 DATAIW010000000
VERSION     DATAIW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184756"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1817
                     /locus_tag="VHM48_07630"
     CDS             189..1817
                     /locus_tag="VHM48_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755316.1"
                     /translation="MNDGAPDPPATVRLGRWQVDRRLLVFIGLAVLARLAFWLITDRV
                     WEDALITVTHARNAVAGVGLTHHPGEPVTHGFTSAVSVLIPLAGEIVVKGSGIFVLRV
                     ASILAAIATIVFADRICRRLGLGRWPTAMALLYLAFDQNQIFYGMAGMETQVAVAVLL
                     WSVDAVMAGTVLRTGLSFGLAVLTRPDFLLWNAAATLDLGARDWRRAARIVVIGALVL
                     APWILFTTLYYGSPLPNTILAKSAHFVSPLPAHLDPASLVGWLQTALTDHVSALSRTF
                     APFYEDTLVSAAPLPLDLAYFVSILVWLLAILGAIRTWRIPGWRAAVLFAAAFTLYRV
                     ALLPGAYFDWYVPPHTAILIILAGAGVHSLRPTPRFDPRPILAVAVAVAFAIHTPFSL
                     GMERKLQADIEVTVRTRVGQELAGLIRPGETLATESAGYYEYYSNATMLDYPGLTSVR
                     ARQAMQSLPPDHKTIIDFLDVVQADWMALRTAEWQGLQQDYPATAARYDVVDQVESPP
                     GSIAFDADGRPRIEFAGYAKDASSWQIVILHRVR"
     gene            complement(1999..3270)
                     /locus_tag="VHM48_07635"
     CDS             complement(1999..3270)
                     /locus_tag="VHM48_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755317.1"
                     /translation="MTATTGSLAPVTTRSSFLTRFDRPAQVGVGVAILAMTIYWLSNR
                     LFDATRGDFFYLADAFLHGRVWLDTRLGFQDVIVRDGHIFVPFAPFPAVALMPIVAIF
                     GPVVADQWETGINAALAASTVGLAWWFAGRAGVRSLVDRFFLVVLLGFSTQIWWVTTR
                     GGVWHTGHLIATILTLACLIELWGSRRAWLIGLLAGAAFMTRAPLAFAIPFYALLLAG
                     DRIWEPRRWPWRTWFELALGFLPMLAFFFWYNAARFGSPLESGYGLALLPDWLEQQRK
                     LGLFSTAHLQMNINYLFLRVPRRIDEFPFFQPDGLGMSIFITSPGLLYAVRARWRESL
                     TWWLALAALIVLIPTLLYYGGGWLQYGYRYALDSIPLVWALCALAAVRDEEARDSVAL
                     EGSAIGTGWRILIGLGVLVGLGGVYWAYNLH"
BASE COUNT          539 a         1249 c         1208 g          501 t
ORIGIN      
        1 ctcgtacatc agcccggcgg cgccggacag gatgaagatc gcaagcacga ggcggggatg
       61 gcgcacggtt cctccagagg ggccggggtc gcgaggcagt gtcccatgcc gcagccgatc
      121 cgtcccgggg cgaacgggac gattgggtgg gcctcgatcg gccggaggca tcggctaggc
      181 tgcaccccgt gaacgacgga gcgcccgacc cacctgccac ggtccgtctt ggccgctggc
      241 aggtcgaccg ccgcctcctc gtcttcatcg gcctggccgt cctcgcccgg ctcgcgttct
      301 ggctgatcac cgaccgcgtc tgggaggacg cgctgatcac ggtgacccac gctcgcaacg
      361 cggtcgccgg ggtcgggctg acccatcacc cgggcgagcc cgtgacccac ggcttcacgt
      421 cggccgtgtc ggtcctgatc ccgctcgccg gcgaaatcgt cgtcaagggc agcgggatct
      481 tcgtgctgcg ggtcgcgtcg atcctggcgg cgatcgcgac gatcgtgttc gccgaccgga
      541 tctgccgccg gctgggtctc gggcgctggc cgactgcgat ggccctcctc tacctcgcgt
      601 tcgaccagaa ccagatcttc tacgggatgg ccgggatgga gacccaggtc gcggtcgcgg
      661 tcctgctgtg gagcgtcgac gcggtgatgg ccgggacggt gctgcggacc gggctgagct
      721 tcggcctagc cgtcctgacc cgcccggact tcctcctctg gaacgccgcg gcgaccctcg
      781 atctcggcgc gcgcgattgg cggagagccg cccggatcgt cgtcatcggc gccctcgtcc
      841 tcgccccctg gatcctcttc acgacgctct actacggctc accgctcccc aacacgatcc
      901 tcgccaagtc ggcccatttc gtgtcgccgc tccccgccca cctcgaccct gcgtcgctcg
      961 tcggctggct ccagaccgcc ctcaccgacc acgtcagcgc cttgagccgg acgttcgcgc
     1021 cgttctacga ggacacgttg gtttcggcgg ccccgctgcc gctcgacctg gcgtactttg
     1081 tgtcgatcct cgtctggctg ctcgcgatcc tcggcgcgat ccggacctgg cgcatccccg
     1141 gctggcgcgc ggcggtcctg ttcgcggcgg cgttcaccct ctatcgcgtc gccctgctgc
     1201 cgggcgccta cttcgactgg tacgtcccgc cgcacacggc gatcctcatc atcctcgcgg
     1261 gcgccggggt ccactcgctg cgaccgaccc cccggttcga cccccgcccg atcctcgccg
     1321 tcgccgtcgc cgtcgccttc gcgatccaca ccccgttctc gctcgggatg gagcggaagc
     1381 tccaggccga tatcgaggtc accgtccgga cccgcgtcgg ccaggagctg gccgggctga
     1441 tcaggcccgg cgagacgctc gcgaccgagt cggccggcta ctacgagtac tactcgaacg
     1501 cgacgatgct cgactacccg gggctcacct cggtccgggc gcgccaggcg atgcagagcc
     1561 tgccgcccga ccacaagacg atcatcgact tcctcgacgt ggtccaggcc gactggatgg
     1621 ccctccggac ggcggagtgg caggggctcc agcaggacta cccggcgacc gccgcccgct
     1681 acgacgtcgt cgaccaggtc gagagcccgc ccggctcgat cgcgttcgac gccgacggcc
     1741 ggcccaggat cgagttcgcc ggctatgcca aggacgcttc gagttggcag atcgtcatcc
     1801 tgcatcgggt ccgttgatcg tggcgtcggc cggcgcgcta cacgccaggc tcagcgccag
     1861 acacaccccc gctggccttt cgaatagccg aatgaatcga gattcgcggt attcggctat
     1921 tcgcaacgcg tgggcggatg gcaacggctg ccgcacacga acggcgcgcc ggacgggcga
     1981 gccgcggccg ggcaggcctc agtggaggtt gtaggcccag tagacgccgc cgaggccgac
     2041 gagcacgccg agcccgatca ggatccgcca gcccgtcccg atcgccgagc cctcgagggc
     2101 caccgagtcg cgggcctcct cgtcgcggac ggctgccagc gcgcacagcg cccagacgag
     2161 cgggatcgag tcgagcgcgt agcggtagcc gtactggagc cagccgccgc cgtagtagag
     2221 cagcgtcggg atcagcacga tcagggccgc cagggccaac caccaggtca gcgactcgcg
     2281 ccaccgggcc cgcacggcat acaggagccc cggtgacgtg atgaagatcg acatcccgag
     2341 cccgtccggc tggaagaacg ggaactcgtc gatccggcgc ggcaccctca ggaacaggta
     2401 gttgatgttc atctggaggt gggcggtcga gaagagaccg agcttgcgct gctgctcgag
     2461 ccagtccggc agcagggcga gcccgtagcc ggactcgagc ggtgacccga accgggcggc
     2521 gttgtaccag aagaagaacg cgagcatcgg caggaacccg agggcgagct cgaaccacgt
     2581 ccgccacggc cagcgccgcg gctcccagat ccggtcgccg gccaggagca gggcgtagaa
     2641 cgggatcgcg aacgcgagtg gggcgcgggt catgaaggcc gccccggcca gcaggccgat
     2701 cagccacgcc cggcgcgacc cccacagctc gatcaggcag gcgagggtca ggatcgtcgc
     2761 gatcaggtgg ccggtgtgcc agacgccgcc gcgggtcgtc acccaccaga tctgggtcga
     2821 gaagccgagc aggacgacga ggaagaaccg gtcgaccagc gagcgcacgc cggcccgccc
     2881 cgcgaaccac caggccagcc cgacggtcga ggcggccagc gcggcgttga tcccggtctc
     2941 ccactggtcg gcgacgaccg gcccgaagat cgccacgatc ggcatgaggg cgaccgccgg
     3001 aaacggcgcg aacgggacga agatgtggcc gtctcggacg atgacgtcct ggaaaccgag
     3061 ccgcgtgtcg agccagaccc ggccgtggag gaacgcgtcg gcgaggtaga agaagtcgcc
     3121 gcgggtcgcg tcgaacaacc ggttcgacag ccagtagatc gtcatcgcca ggatcgcgac
     3181 gccgaccccg acctgggcgg gccggtcgaa gcgggtcagg aacgatgagc gcgtcgtgac
     3241 gggcgcgagc gatccggtgg tggccgtcat gggccgccag tgtgccacct gcacctgctt
     3301 cgatgagcga tcggcagcgc gaccgatggc gtcgtaccag ggtcgcgccc gaaccgcggt
     3361 gatcaggtcg ggcgaaggat tgcgatcgtg gttccgttgc tgtcgccgtc ggggtcgtgg
     3421 ttggccgccg gtgtgtaggt tccgccggat cgggtcacgt tcgtcacggt gaacgtcacg
     3481 ctcgcaacgg ccgaccg
//
LOCUS       DATAIW010000243         5557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_244240, whole genome shotgun sequence.
ACCESSION   DATAIW010000243 DATAIW010000000
VERSION     DATAIW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244240"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VHM48_07640"
     CDS             <1..1604
                     /locus_tag="VHM48_07640"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX2755318.1"
                     /translation="DARAAFREQIEGLLEGGADAIVLETFSDLGSLLLAVDEARRASD
                     VPVIASLTFGEELVLADGTGPAAAAAALGRAGADAVGVNCGAGPTPCLDALEAMGEPA
                     EGEPARSIMPNAGLSQRLEGRFVFAASPEYFGTVTPRMLAAGARILGGCCGTTPEHIA
                     AMRLALDADAVAGTADGRDAELVGQGAAGAPAAPAPSIVVVPGAGPTASLAERAARPT
                     RADDAPPPTRLAALLAARRFVVSVEIDPPRSIRIERTIEAARLLRDAGVDLVNVSDSA
                     MARVRMSALSVAFGIQHDLDLECLVHCTTRDRNLMALESELLGAHALGVRNVIALTGD
                     PPRIGDYPTGTGVWDVDSIGLIEILGRLNRAEDPSGGSIGQRAGFTIACALDSTAADA
                     ATEWDRLERKLAAGAHLVMTQPLYSVGQVESMLEEAHRRFGPGGFPVPLLLGVLPLVS
                     TRHAEFLHNEVPGITIPDDARAAIRDAGERGSEVGIEMADRLLAAVEGEVAGTYIMPS
                     FVRYEQCADLVRWIRARHPAVAVAS"
     gene            1667..5386
                     /gene="metH"
                     /locus_tag="VHM48_07645"
     CDS             1667..5386
                     /gene="metH"
                     /locus_tag="VHM48_07645"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX2755319.1"
                     /translation="MAALPGILRERIVVLDGAMGTMLQAYGFDEADFRGDRFRDHPRD
                     LRGNSDLLCLTQPAAVSAIHAGYLAAGAEILSTNSFTATRIAQADYGLDPATVRELNV
                     AAARLARDAADAAERADPDRPRFVAGSLGPTNRTASMSADVADPAARSVTWDELALAY
                     RESAAGLIEGGADLLLIETIFDTLNAKAAIFAVQSLFDEIGQRLPLIISGTIVDASGR
                     TLSGQTVEAFWHSIRHADPLIVGLNCALGPRQLREHLDVLSRVADRPVSAYPNAGLPN
                     ELGGYDETPEEMAAAIGEWAQHGLLNVAGSCCGSTPAHVAAIAEAVAGLPPRSIPEPT
                     GMTRLAGLAPLVIPPPGNAFVNVGERTNVTGSRKFARLVAAGLEDEAVDVAREQVANG
                     AQLIDVNMDEAMLDGPAAMARFLRRIAAEPDIATVPVMVDSSKWSVIEAGLHQLQGKG
                     VVNSISLKEGEAEFLRQARLCRRYGAAVVVMAFDEQGQAESVERRVAVLRRAYELLTG
                     VVGFAPEDIILDANIFAIATGMEEHNAYAVSFIETVKRLKEEFPGTRTSGGVSNVSFA
                     FRGNDRVREAIHSVFLYHAIRAGLDMAIVNAGVLPIYDDIEPDLRERVEDVVLNRRPD
                     ATERLLDLAQRYAGESGMERAAEDLAWRERPVEERLTHALVAGIDAFIAEDTEEARVA
                     AGRPLDVIEGPLMAGMNVVGDLFGAGRMFLPQVVKSARVMKKAVAVLIPYLEAERAGT
                     GKRSGTIVTATVKGDVHDIGKNIVGVVLGCNDYEVVDLGVMVPAARILEKAIEIDADL
                     IGLSGLITPSLDEMAHVASEMERQGFTIPLLIGGATTSRMHTAVKIAPNYSGPVVHVL
                     DASRAVGVAGALVQADRRDAFVAGIREEYETVRRERAGRQAKEVRLTIAEARANRVPI
                     DWAQVRPPRPTFLGPRTFTDYPLAELVDFIDWTPFFATWELRGAYPAILDDPRLGTAA
                     RDLHRDALALLERIVADGRLKASAVVGFWPANAVDGDDIVVWRDEDRRVPLATFRTLR
                     QQMAKLAGRPNVALADFVAPTETGISDFIGAFAVTAGHGVEEIVAEFEAANDDYSAIL
                     AKALADRLAEAFAEHLHQRVRRELWGYAPDEALSNADLIAEHYAGIRPAPGYPACPDH
                     TEKATLFELLEAEPRAGIRLTESFAMWPGASVSGYYLWNPASAYFGVGRIGRDQLADY
                     AARKGTPLADAERWLAPNLEDDPGGEDAPPTARPD"
BASE COUNT          752 a         2066 c         1957 g          782 t
ORIGIN      
        1 ccgacgcccg ggcggccttc cgcgagcaga tcgagggcct cctcgagggt ggcgccgacg
       61 cgatcgtcct cgagaccttc tcggatctcg ggtcgctgct gctcgccgtc gacgaggcac
      121 gccgggcgtc ggacgtcccg gtgatcgcct ccctgacgtt cggcgaggag ctcgtcctgg
      181 ccgacggcac cggcccggcg gccgcggccg cggcgctggg ccgcgcgggt gccgatgcgg
      241 tcggggtgaa ctgcggggcg gggccgacac cgtgtctcga tgcgctcgag gcgatgggtg
      301 aaccggccga gggcgagccc gcccgctcga tcatgccgaa cgcggggttg tcgcagcggc
      361 tcgagggacg cttcgtgttt gccgccagcc ccgagtactt cgggacggtg acgccgcgga
      421 tgcttgcggc cggtgcccgg atcctcggcg gctgctgcgg gaccacgccc gagcacatcg
      481 cggcgatgcg gcttgccctc gacgcggacg cagtggcggg gaccgcagac gggcgcgacg
      541 ccgagctcgt tggtcaaggg gcggccggcg cgccggccgc gccggcaccg tcgatcgtcg
      601 tggtcccagg ggcaggccca accgcctccc tggccgagcg agccgcccgc ccgacccggg
      661 ccgacgacgc accgcccccg acccggctgg ctgcgttgct cgccgcgcgc cgcttcgtcg
      721 tgtcggtcga gatcgacccg ccccgaagca tccggatcga gcgcacgatc gaggcggccc
      781 ggctcctgcg cgacgccggt gtcgacctcg tcaacgtctc cgactcggcg atggcccggg
      841 tccggatgag cgccctgtcg gtggcgttcg ggatccagca cgacctcgac ctcgagtgcc
      901 tggtccattg cacgacgcgc gaccggaacc tgatggccct cgagtcggag ctgctcggcg
      961 cccacgccct cggcgtccgc aacgtgatcg ctctgaccgg cgatccgccg aggatcggcg
     1021 actacccgac cgggaccggg gtctgggatg tcgattcgat cggcctgatc gagatccttg
     1081 gccggctgaa ccgggccgag gacccatccg gcgggtcgat cggccagcgg gccggcttca
     1141 cgatcgcctg tgccctcgac tcgacagcgg ccgacgccgc gaccgagtgg gaccggctcg
     1201 agcgcaagct ggccgccggc gcgcacctcg tcatgaccca gccgctgtac tccgtcgggc
     1261 aggtcgagtc gatgctcgag gaggcgcacc ggcggttcgg gccgggcggc tttccggtgc
     1321 ccctgctcct cggagtgctc ccgctcgtct cgacccgcca cgccgagttc ctccacaacg
     1381 aggtccccgg gatcacgatc ccggacgacg cgcgagcggc gatacgcgac gccggcgagc
     1441 gcgggtcgga ggtcgggatc gagatggccg accgcctcct tgcggccgtc gagggcgagg
     1501 tcgccgggac atacatcatg cccagcttcg tgcgctacga gcagtgcgcg gatctcgtcc
     1561 gctggatccg ggcgcggcat ccggccgtcg ccgtggcgtc gtgaccgctg ccgcgccgcc
     1621 cgccgccgag ccgggcttga gctcgccgtt cgatcgcgcc tctcgcgtgg ccgcgctgcc
     1681 gggcatcctc cgcgagcgga tcgtggtcct cgacggggcg atgggaacga tgctccaggc
     1741 ctacgggttc gacgaggccg acttccgcgg cgatcgcttt cgcgaccacc cgcgcgacct
     1801 ccgcggcaac agcgacctcc tgtgcctcac ccagcccgcg gcggtctcgg cgatccacgc
     1861 cggctatctc gccgccggcg ccgagatcct cagcacgaac tcgttcacgg cgacgcggat
     1921 cgcccaggcg gactacgggc tcgacccggc gaccgtccgt gagctgaacg tcgcggccgc
     1981 ccggctcgcc cgcgacgcgg cggacgcggc ggaacgggcc gatccggatc gcccgcgttt
     2041 cgtggccggc tccctcggcc cgacgaaccg gacggcatcg atgagcgcgg acgtcgccga
     2101 tccggccgcc cgcagcgtga cgtgggacga gctcgcgctc gcctatcggg aatccgccgc
     2161 cgggctgatc gaaggcggcg ccgatctcct gctgatcgag acgatcttcg acacgctcaa
     2221 cgcaaaggcg gcgatcttcg cggtccagtc gctgttcgac gagatcggcc aacgcctgcc
     2281 gctgatcatc tcggggacga tcgtggacgc gtcggggcgg accctgtccg gtcagacggt
     2341 cgaggccttc tggcacagca tccgccatgc cgacccgctg atcgtcgggc tcaactgtgc
     2401 ccttggcccg cgtcagctgc gcgagcatct cgatgtcctc tcgcgggtcg ccgaccggcc
     2461 ggtgtcggcg tatcccaacg ccggcctgcc caacgagctc ggcgggtatg acgagacccc
     2521 cgaggagatg gccgccgcga tcggcgaatg ggcccagcac ggcctgctga acgtcgccgg
     2581 cagttgctgc gggtcgacgc cggcccacgt cgccgcgatc gcggaggccg tcgccggcct
     2641 gccgccgcgc tcgatcccgg agccgaccgg catgacccgc ctcgccggcc ttgccccgct
     2701 ggtcatcccg cccccgggca acgcgttcgt gaatgtcggg gagcggacga acgtcaccgg
     2761 ctcgcggaag ttcgcccggc tcgtcgccgc cggtctcgag gacgaggcgg tcgacgtcgc
     2821 ccgggagcag gtcgcgaacg gcgcccagct catcgacgtg aacatggacg aggcgatgct
     2881 cgacggtccc gccgcgatgg cccggttcct gcgccggatc gcggccgagc cggacatcgc
     2941 caccgtcccg gtcatggtcg acagctcgaa gtggtcggtc atcgaggccg gtctgcacca
     3001 gctccagggg aagggcgtcg tcaactcgat ctccctcaag gagggcgagg ccgagttcct
     3061 ccgccaggcc cggctctgcc ggcggtacgg tgcggcggtc gtcgtcatgg ccttcgacga
     3121 gcagggccag gccgaatccg tcgagcgtcg ggtggcggtc ctccgccgcg cctacgagct
     3181 gctgacaggc gtcgtcgggt tcgcccccga ggacatcatc ctcgacgcca acatcttcgc
     3241 gatcgcgacc ggcatggagg agcacaacgc ctacgcggtg tcgttcatcg agaccgtcaa
     3301 gcggctgaag gaggagttcc cggggacgcg gacctccggc ggcgtgtcga acgtgtcctt
     3361 cgccttccgc ggcaacgacc gggtccgcga ggcgatccac tcggtcttcc tctaccacgc
     3421 gatccgggcc ggcctcgaca tggcgatcgt gaacgccggc gtcctgccga tctacgacga
     3481 catcgagccg gatctccgcg agcgggtcga ggacgtcgtc ctcaaccgcc gcccggacgc
     3541 caccgagcgt ctgctcgacc tcgcccagcg gtacgcgggc gagagcggca tggagcgtgc
     3601 cgccgaggac ctggcgtggc gcgagcgacc ggtcgaggag cggctcaccc acgccctcgt
     3661 cgccggcatt gacgccttca tcgccgagga caccgaggag gcccgcgtgg cggccggccg
     3721 gccgctcgac gtcatcgagg gcccgctgat ggcagggatg aacgtcgtcg gcgacctgtt
     3781 cggcgccggc cggatgttcc tgccccaggt cgtcaagagc gcccgggtca tgaagaaggc
     3841 cgtggccgtc ctgattccgt atctcgaggc ggagcgggcc ggcacgggca agcgatccgg
     3901 gacgatcgtg accgcgacgg tcaagggcga cgtccacgac atcggcaaga acatcgtcgg
     3961 ggtcgtcctc ggctgcaacg actacgaggt ggtcgatctc ggggtcatgg tcccggcggc
     4021 gaggattctc gagaaggcga tcgagatcga cgcggacctc atcggactgt ccgggctgat
     4081 cacgccgtcg ctcgacgaga tggcccacgt cgcctcggag atggagcgcc agggcttcac
     4141 gatcccactg ctcatcggcg gcgcgacgac ctcgcggatg cacaccgcgg taaagatcgc
     4201 gccgaactac tcgggtccgg tcgtccacgt cctcgatgcg tcgcgggcgg tcggcgtggc
     4261 cggcgccctc gtccaggcgg accgtcgcga cgcgttcgtc gccggcatcc gcgaggagta
     4321 cgagaccgtc cgccgagagc gggccggccg gcaggcgaag gaggtgcggc tcaccatcgc
     4381 cgaggcccgc gccaatcgcg tcccgatcga ctgggcccag gttcgccccc cgcggccgac
     4441 gttcctcggc ccgcggacct tcaccgacta ccccctcgcc gagctcgtcg acttcatcga
     4501 ctggacgccg ttcttcgcga cctgggagct gcgcggtgcc tatcccgcga tcctcgacga
     4561 cccgcggctg ggcacggcgg ctcgcgatct ccaccgggac gcgctcgccc tcctcgagcg
     4621 gatcgttgcc gacggccggc tcaaggcgag tgccgtcgtc ggcttctggc cggccaacgc
     4681 cgtcgacggg gacgacatcg tcgtgtggcg tgacgaggac cgccgcgtgc cgctcgcgac
     4741 cttccggacc ctccgccagc agatggccaa gctggcgggt cgcccgaacg tcgccctggc
     4801 cgacttcgtc gccccgaccg agaccgggat ctccgacttc atcggcgcct tcgccgtcac
     4861 cgccggccac ggtgtcgagg agatcgtcgc cgagttcgag gccgccaacg acgactactc
     4921 ggcgatcctc gccaaggccc tcgccgaccg cctggccgag gccttcgccg agcacctcca
     4981 ccagcgcgtc cggcgcgagc tgtggggcta cgcccccgac gaggccctct cgaatgccga
     5041 cctgatcgca gagcactacg cgggcatccg gccggcgccc ggctatccgg cctgtcccga
     5101 ccacacggag aaggcgacgc tgttcgagct cctcgaggcc gagccacggg ccgggatccg
     5161 gctgaccgag tcgttcgcga tgtggccggg cgcgtcggtc tccggctact acctctggaa
     5221 ccccgccagt gcctatttcg gcgtcgggcg gatcggtcgc gaccagctcg ccgactacgc
     5281 ggcccgcaag ggaaccccgc tcgccgacgc ggagcgctgg ctcgccccga acctcgagga
     5341 cgacccggga ggcgaagacg ccccaccgac ggcccgaccc gactgaggcg tcctcggcca
     5401 ggccgctcgc tcgacgcccg ccatcgcggc gccgcgcgtg gctcggttcg ggcgtcacca
     5461 cggcccggcg ctcgccgtaa cctgagggaa cgccgaccgc ggctgagggg aggcacatgc
     5521 gtcgcacaac cgggctcgtc gtcgcggtcc tcctggc
//
LOCUS       DATAIW010000244         8183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_13499, whole genome shotgun sequence.
ACCESSION   DATAIW010000244 DATAIW010000000
VERSION     DATAIW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13499"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4502)
                     /gene="rpoC"
                     /locus_tag="VHM48_07650"
     CDS             complement(<1..4502)
                     /gene="rpoC"
                     /locus_tag="VHM48_07650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX2755320.1"
                     /translation="MLEVNNFSAIRISLASPDQIREWSKGEVTKPETINYRTLKPEKD
                     GLFDERIFGPTKDWECYCGKYKRIRYKGIICDKCGVEVTRSKVRRERMGHIQLASPVS
                     HIWYFKGTPSRLGILLDISPRNLERILYFALYIVTNVDEEARKRALLALEDEAAGRGG
                     KAGEALAELEDRLRADVNRTKDELTAALAVTKADLESQRTVRTEEVVTAAQAVQAQLA
                     ALKTGEAEDTIVFAPTGEVVVAAGAKGGKEATAALRKIVSAETERVTAELKQRERDEE
                     RAVEQKVADLSASVDETLRNEREQLSGGAQAAKDEVKKLRDEIEGLKPMQTLGELELR
                     GLEERHGSGAKGGRLFNAGMGAEAVREIISRMDLEELSRSLHVEVRTSSGQRRKKAIK
                     RLRLIEAFRRSGTRPDWMILSVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNN
                     RLKRLLELGAPEIIIRNEKRMLQEACDALIDNGRRGRAIAGTGNHRLKSLSDMLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVMRQLVEKGFAHN
                     IKSAKRIVERVRPEVWDVLEEVIKDHPVLLNRAPTLHRLGIQAFMPVLVEGSAIQIHP
                     LVCTAFNADFDGDQMAVHVPLSTAAQEEARTMMLSTANLLSPADGSPVVAPTQDMVLG
                     CYYLTMDPIGKQPKRVRIFATEDEAILSYQLKEKTGVTLHEPVEAEVRTWDAEAGQLV
                     LRRVRTTVGRIIFNQILPDQMRFKDEVMKRSDLKDLVDECYRLLGPDETAHLVDGIKS
                     VGFDFATRGGMTIGLFDIEVPKDKPARLAAADASVAEIDRQFQRGLITDDERYEQVVD
                     VWQKTTNAMSDEMMKGLKVDGPVTMMTASGARGNKGNIGQLGAMRGLMADPSGRIIDV
                     PVRSNFREGMTVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVAQDVITRDDDCG
                     TEEGSWITREDSTDEKEFRRRLVGRLAAAPLPDSSVKLKKGAEQPLVVDRNIEITEEI
                     AVRIEAAGIQEVLVRSPLTCEARYGVCRACYGRNLATGDMVASGEAVGIIAAQSIGEP
                     GTQLTMRTFHTGGVAGLDITAGLPRVEELFEARVPKGKAEISHIDGIVEIIRSDTGTK
                     VKVVSTEVYDTTLGLPKGAEMLAAAGDSVEANQVIARIADGDTGGPTDVASPVKGFLV
                     KGDDGLAIRAEDVVEREYTIPHNAKLLVENGQEIRAGDGITDGPINPQEYLETRGKDA
                     VQRYLVKEVQKVYRSQGVTINDKHIEIIVRQMLRKVRIDQPGDVDLLPTELIDRLDFE
                     EENNKVLSEGGEPATAQTVLLGVTKASLNTSSFLAAASFQETTRVLTEAAINGAKDHL
                     IGLKENVIIGKLIPAGTGAPANVAARKERDRRAALEALAGEAIEDTGSEYNPFLEEGG
                     RPADEAAGLALAATIAGASEEGEFNPFLSDDEASDDEDDATATDNPFVAVGASGGAAD
                     AGADKAS"
     gene            complement(4613..8092)
                     /locus_tag="VHM48_07655"
     CDS             complement(4613..8092)
                     /locus_tag="VHM48_07655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2755321.1"
                     /translation="MLSAESSNRTAVALARTRYARIPEVLPIPNLIELQLDSFQWFIE
                     KGLRELFDEISPIKDFTAKVMELQFLDYEFGEPKYSEEECRTKDLTYSRPLYVNVELL
                     IKETGEIQRQRVYMGDYPWMTNQGTFVINGAERVVVSQLVRSPGVYYSELEDPASGRM
                     LFSAKVIPNRGAWLEFETNNKDQLWVKVDRKRKIAATTLLRAVGYEENDSIAALFSGV
                     DADPDHPFTANTLDKDLTKTQQEALIEVYKKLRPGDPPTGDNARQLVESLFFNFRRYD
                     LGRVGRYKFNKKLDGVAGRMGIELPREQRTITREDIAAIVGNLIESNRGLHPKDDIDH
                     LGNRRIRANGELIQNAFRIGLLRMERVVRERMTIQEIDKATPNALINIRPVVAAMKEF
                     FGGSQLSQFMDQTNPLAELTSKRRLSALGPGGLSRERAGFDVRDVHHSHYGRICPIET
                     PEGPNIGLIGSLATYGRINSYGFIETPYRKVRRTVGWDDPNIATYEAGADLLTKGGET
                     ILKTGQPFTTAAIAELKKQKANAFPIRPIVTADIEYLPADEEEEHVVAQANAPLDAEG
                     HFLAERIPSRYRDTFPEARAGQIEYMDVSPKQVVSVATALIPFLEHDDANRALMGSNM
                     QRQAVPLLEPESPIVGTGMEERAARDSGQVVVARRGGVVTSVTAERIRIETDAGELDE
                     YKLQKFVRSNQGTCINQRPIVDVGHRVADGQALADSSSTQEGELALGRNVLVAFMSWE
                     GGNYEDAIVISDRLVRDDLFTSIHIEKHEIESRDTKLGPEEITRDIPNVGEESLKDLD
                     EEGIVYIGAEVRPGDILVGKITPKGETELTAEERLLRAIFGEKAREVKDSSLRLPHGE
                     RGKVVEIREFRRELNDDLQPGVNRLVRVSVAQKRKISVGDKMAGRHGNKGVIAKILPA
                     EDMPFLPDGTPVDIILNPLGVPSRMNIGQILETHLGWALHEKGQKAATAVFDGATEDQ
                     IKAELREARHKVYGVEKALPEDGKIDLRDGRTGEIFDRPITVGYIYMLKLHHLVEDKI
                     HARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQELLTVKSDDVLGR
                     VQTYEAIVKGEEIQPPRVPESFKVLIKELRSLGLNAEILDQNDEEIQLAEDDVSGYML
                     PDLGGINLAGFED"
BASE COUNT         1100 a         2677 c         2873 g         1533 t
ORIGIN      
        1 gacgccttgt cggcgcccgc atcggccgct cccccgctcg cgccgacggc gacgaacggg
       61 ttgtcggtgg cggtggcatc gtcctcgtcg tcgctcgcct cgtcatcgga gaggaacggg
      121 ttgaactcgc cctcctccga cgcgcccgcg atcgtcgccg cgagagccag gccggcggcc
      181 tcgtcggccg gccggccgcc ctcctcgagg aacgggttgt actcgctgcc ggtgtcctcg
      241 atcgcctcgc cggcgagggc ctcgagggcc gcccggcggt cgcgctcctt gcgggccgcg
      301 acgttggccg gcgcgccggt cccggcgggg atcagcttgc cgatgatgac gttctccttg
      361 agcccgatca ggtggtcctt ggcgccgttg atggccgcct cggtgagcac ccgggtcgtc
      421 tcctggaagg acgccgcggc gaggaaggac gaggtgttga gagatgcctt ggtcaccccg
      481 aggagcacgg tctgggccgt cgccggctcg ccgccctcgg acaggacctt gttgttctcc
      541 tcctcgaagt cgagccggtc gatcagctcg gtcgggagca ggtcgacgtc gcccggctgg
      601 tcgatccgga ccttccggag catctggcgg acgatgatct cgatgtgctt gtcgttgatc
      661 gtgacgccct ggctccggta gaccttctgg acttccttga ccaggtagcg ctggacggcg
      721 tccttgcccc gggtctcgag gtactcctgc gggttgatcg ggccgtccgt gatcccgtca
      781 ccggcccgga tctcctggcc gttctcgacg agcagcttgg cgttgtgggg gatcgtgtat
      841 tcgcgttcga cgacgtcctc ggcccgaatg gcgagcccgt cgtcgccctt gaccaggaag
      901 cccttgaccg gcgacgcgac atcggtcggc ccgccggtat cgccgtcggc gatccgggcg
      961 atgacctggt tggcctcgac cgaatccccg gccgcggcca gcatctcggc gcccttcggc
     1021 aggcccagag tcgtgtcgta gacctccgtg ctcaccacct tgaccttggt cccggtgtcg
     1081 ctgcggatga tctcgacgat cccgtcgatg tggctgatct cggccttgcc cttggggacg
     1141 cgggcctcga acagctcctc gacgcggggc agaccggcgg tgatgtccag gccggcgacg
     1201 ccgccggtat ggaacgtccg catggtcagc tgggtgcccg gctcgccgat cgactgggcg
     1261 gcgatgatcc cgacggcctc cccggaggcg accatgtcgc cggtcgcgag gttacggcca
     1321 tagcacgccc ggcagacgcc gtagcgagct tcgcaggtca gcggtgagcg gacgaggact
     1381 tcctggatcc cggccgcctc gatgcggacc gcgatctcct cggtgatctc gatgttgcga
     1441 tcgacgacga gcggctgctc cgcgcccttc ttgagcttga cggacgaatc cggcagcggc
     1501 gcggcggcca ggcggccgac gagccggcga cggaactcct tctcgtcggt cgagtcctcg
     1561 cgggtgatcc agctgccttc ctcggtgccg cagtcgtcat cgcgggtgat gacgtcctgg
     1621 gccacgtcga cgaggcgtcg ggtcaggtag ccggagtcgg ccgtccggag ggcggtgtcg
     1681 gcgaggccct tgcgggcgcc gtgggtcgag atgaagtact cgaggacggt catcccctcc
     1741 cggaagttgc tccggacggg gacgtcgatg atccggcccg acgggtcggc catcagtccg
     1801 cgcatcgcgc ccagctggcc gatgttcccc ttgttgccgc gggctccgga cgcggtcatc
     1861 atcgtgaccg ggccgtcgac cttgaggccc ttcatcatct cgtcggacat cgcgttcgtc
     1921 gtcttctgcc agacgtcgac gacctgctcg tagcgctcgt cgtcggtgat caggccgcgc
     1981 tggaactggc gatcgatctc ggcgaccgag gcgtcggctg ccgcaaggcg ggccggcttg
     2041 tccttcggga cctcgatgtc gaacaggccg atcgtcatcc cgccacgggt ggcgaagtcg
     2101 aagccgacgc tcttgatccc gtccacgagg tgggcggtct cgtcgggccc gagaaggcga
     2161 tagcactcgt cgacgaggtc cttgaggtcg gaccgcttca tgacctcgtc cttgaaccgc
     2221 atctggtccg gcaggatctg gttgaagatg atccggccga ctgtcgtccg gacgcgacgg
     2281 aggacgagct ggcccgcctc ggcgtcccag gtccggacct cggcctcgac cggctcgtgg
     2341 agcgtgacgc cggtcttctc cttgagctga taggacagga tcgcctcgtc ctcggtcgcg
     2401 aagatccgga cgcgcttggg ctgcttgccg atgggatcca tcgtcaggta gtagcagccg
     2461 aggaccatgt cctgggtcgg cgccacgacc ggcgagccgt cggcaggcga caggaggttg
     2521 gcggtcgaca gcatcatcgt ccgcgcttct tcctgggcgg ccgtcgagag cggcacgtgg
     2581 accgccatct ggtcgccgtc gaagtccgcg ttgaacgcgg tgcagacgag cgggtggatc
     2641 tggatcgccg agccctcgac gaggaccggc atgaacgcct ggatgccgag gcgatggagg
     2701 gtcggggcgc ggttcagcag gaccggatgg tccttgatga cctcttcgag gacgtcccag
     2761 acttccggcc ggacgcgctc gacgatgcgc ttggcgctct tgatgttgtg ggcgaagccc
     2821 ttctcgacga gctggcgcat gacgaacggc ttgaacagct cgagcgccat cttcttgggc
     2881 aggccgcact ggtggagctt gagctccggc ccgacgacga tgaccgaccg gcccgagtag
     2941 tcgacgcgct tgccgagcag gttctggcgg aaccggccct gcttgccctt gagcatgtcg
     3001 gacaggctct tgaggcggtg gttgccggtg ccggcgatcg cccggccacg gcggccgttg
     3061 tcgatcaggg cgtcgcacgc ctcctggagc atccgcttct cgttgcggat gatgatctcg
     3121 ggcgcaccga gctcgaggag ccgcttgagg cggttgttgc ggttgatcac ccgccgatag
     3181 aggtcgttca ggtcggaggt cgcgaagcgc ccgccgtcga gctggaccat cggccgcagg
     3241 tccggcggga tcaccggcag gaccgacagg atcatccagt cgggccgggt gccgctgcgt
     3301 cggaaggcct cgatcaggcg gagccgcttg atcgccttct tgcgccgctg gccggagctc
     3361 gtccggacct cgacgtggag ggatcgcgag agctcctcga ggtccatccg gctgatgatc
     3421 tcgcggacgg cctccgcgcc catcccggcg ttgaacaggc ggccaccctt ggcgccggag
     3481 ccgtggcgct cctcgagccc acgcagctcg agctcgccga gcgtctgcat cggcttgagg
     3541 ccctcgatct cgtcgcggag cttcttgacc tcgtccttcg ctgcctgggc tccgcccgag
     3601 agctgctcgc gctcgttgcg gagggtctcg tcgacgctgg cgctgaggtc ggcgaccttc
     3661 tgctcgaccg cccgctcctc gtcgcgctcg cgctgcttga gctcggcggt gacccgctcg
     3721 gtctcggccg acacgatctt gcggagggcg gcggtcgcct ccttgccgcc cttggcgccg
     3781 gcggccacga cgacctcgcc ggtcggggcg aagacgatcg tgtcctcggc ctcgccggtc
     3841 ttgagggcgg cgagctgggc ctggacggcc tgggccgccg tcacgacctc ctcggtccgg
     3901 acggtgcgct ggctctcgag gtcggccttc gtcacggcga gggcggcggt cagctcgtcc
     3961 ttggtcctgt tgacgtccgc gcggaggcgg tcctcgagct cggcgagcgc ctcgccggcc
     4021 ttgccgccgc gtcccgcggc ctcatcctcg agggcgagga gggcgcgctt gcgggcctcc
     4081 tcgtcgacgt tcgtgacgat gtacagggcg aagtagagga tgcgctcgag gtttcgcggg
     4141 ctgatgtcga ggaggatgcc gagtcggctc ggggtgccct tgaaatacca gatgtggctg
     4201 accgggctgg ccagctggat gtggcccatg cgctcacggc ggaccttcga gcgggtgacc
     4261 tcgacgccgc acttgtcgca gatgatgccc ttgtagcgga tccgcttgta cttgccgcaa
     4321 tagcactccc agtccttggt cggaccgaag atgcgttcgt cgaagagacc atccttttcc
     4381 ggcttgagcg tccggtagtt gatggtctcc ggcttcgtca cctcaccctt tgaccactcc
     4441 cggatctggt ccgggctggc gagcgagata cggatcgcgc tgaaattatt gacctcgagc
     4501 atggttctcc tccgcgccgc cgcctggccc ggcggtgcga tgcgacctca ggacgcgtga
     4561 ggccggtccg gtcgggcctc cacccgacca gtgcgcaatg aatgcagagc cgtcagtcct
     4621 cgaacccggc gagattgatc ccgccgagat ccgggagcat gtagccggag acgtcgtctt
     4681 cggcgagctg gatctcctcg tcgttctggt cgaggatctc ggcgttcaat ccgaggctgc
     4741 ggagctcctt gatcaggacc ttgaaggatt ccgggacgcg cggcggctgg atctcctccc
     4801 ccttgacgat ggcttcatag gtctggacgc gtccgaggac gtcgtccgac ttcacggtga
     4861 gcagttcctg aaggatgttc gcggcgccgt acgcctcgag tgcccagacc tccatctcgc
     4921 cgaaccgctg gccgccgaac tgggccttgc caccgagcgg ctgctgggtg atcaggctgt
     4981 acgggccggt cgagcgggcg tggatcttgt cctcgacgag gtggtggagc ttgagcatgt
     5041 agatgtagcc gacggtgatc gggcggtcga agatctcgcc ggtccggccg tcgcgaaggt
     5101 cgatcttgcc gtcctccggg agggccttct cgacgccgta gaccttgtgg cgggcctccc
     5161 gcagctcggc cttgatctgg tcctccgtcg cgccgtcgaa gaccgcggtg gcggccttct
     5221 ggcccttctc gtgaagggcc cagccgagat gtgtctcgag gatctggccg atgttcatcc
     5281 ggctcggcac gccgagcggg ttgaggatga tgtcgaccgg cgtcccgtcc ggcaggaacg
     5341 gcatgtcctc cgccggcagg atcttggcga tgacgccctt gttgccgtgg cggccggcca
     5401 tcttgtcgcc gacgctgatc ttccgcttct gggcgaccga gacccggacc aggcggttga
     5461 cgcccggctg gaggtcgtcg ttgagctcgc gccggaactc gcggatctcg acgaccttgc
     5521 ctcgctcgcc gtgcggtagg cggaggctcg agtccttcac ctcgcgggcc ttctcaccga
     5581 agatcgcccg caggaggcgc tcctcggcgg tcagctcggt ctcgcccttg ggcgtgatct
     5641 tgccgacgag gatgtcgccg gggcggacct cggcgccgat gtacacgatg ccctcttcgt
     5701 cgaggtcctt gagggactcc tcgccgacgt tcgggatgtc gcgggtgatc tcctcgggcc
     5761 cgagcttggt gtcgcgcgac tcgatctcgt gcttctcgat gtggatgctg gtgaagaggt
     5821 cgtcgcggac gaggcggtcg ctgatgacga tcgcgtcctc gtagttgccg ccctcccagc
     5881 tcatgaacgc cacgaggacg ttccggccga gggcaagctc gccctcctgg gtcgacgagc
     5941 tgtcggcgag cgcctggccg tccgcgacgc ggtggccgac gtcgacgatc gggcgctggt
     6001 tgatgcaggt gccctggttg ctgcggacga acttctggag cttgtactcg tcgagctcac
     6061 cggcatcggt ctcgatccgg atccgctcgg ccgtcacgct ggtgacgacg ccgccgcggc
     6121 gggcgaccac gacctggccg gagtcacgcg cggcgcgctc ctccatcccg gtcccgacga
     6181 tcggcgactc cggctcgagg agcgggactg cctggcgctg catgttcgag cccatgaggg
     6241 cgcggttggc gtcgtcgtgc tcgaggaacg ggatcagcgc ggtggccacg gacacgacct
     6301 gtttggggct gacgtccatg tattcgatct ggccggcacg cgcctcgggg aacgtgtcgc
     6361 gatagcgcga cgggatgcgc tcggcgagga agtggccctc ggcgtcgagc ggggcgttgg
     6421 cctgggcgac gacgtgctcc tcctcctcgt cggccgggag gtactcgatg tcggcggtca
     6481 cgatcgggcg gatcgggaag gcattggcct tctgcttctt cagctcggcg atcgccgcgg
     6541 tcgtgaacgg ctggccggtc ttgaggatcg tctcaccgcc cttggtcagg aggtcagcgc
     6601 cggcctcgta ggtggcgatg ttcgggtcat cccagccgac cgttcgcctg accttgcgat
     6661 agggggtctc gatgaagccg tagctgttga tccggccata ggtcgcgagc gacccgatca
     6721 acccgatgtt cgggccctcg ggcgtctcga tcgggcagat ccggccgtag tggctgtggt
     6781 ggacgtcgcg gacgtcgaac ccggcgcgct cgcgcgacag gccgcccggc ccgagggcgc
     6841 tcaggcgccg cttcgaggtg agctcggcga gcgggttcgt ctggtccatg aactgggaca
     6901 gctggctgcc gccgaagaac tccttcatcg cggccacgac cggccggatg ttgatgaggg
     6961 cgttcggggt cgccttgtcg atctcctgga tcgtcatccg ctcgcggacg acccgctcca
     7021 tccggaggag gccgatccgg aacgcattct ggatcagctc gccgttggcc cggatccggc
     7081 ggttgccgag gtggtcgatg tcgtccttgg gatggaggcc ccggttcgac tcgatcaggt
     7141 tgccgacgat cgccgcgatg tcctcgcgcg tgatcgtccg ctgctcgcgc ggcagctcga
     7201 tgcccatccg cccggcgacg ccgtcgagct tcttgttgaa cttgtagcgg ccgacccgac
     7261 cgaggtcgta gcggcggaag ttgaagaaca ggctctcgac cagctggcgg gcgttgtcgc
     7321 cggtcggcgg atcgcccggg cggagcttct tgtagacctc gatcagggct tcctgctggg
     7381 tcttcgtcag gtccttgtcg agggtgttgg ccgtgaacgg atgatccggg tcggcgtcga
     7441 cgcccgagaa gagcgcggcg atggagtcgt tctcctcgta gccgacggcc cgcaggaggg
     7501 tcgtcgccgc gatcttgcgc ttgcgatcga ccttgaccca gagctggtcc ttgttgttgg
     7561 tctcgaactc gagccacgcg ccacggttcg ggatgacctt ggcactgaac agcatccgcc
     7621 cggacgccgg gtcctcgagc tcgctgtagt agacgccggg cgagcggacg agctggctga
     7681 ccacgacgcg ctcggcgccg ttgatcacga acgtgccctg gttggtcatc cacgggtagt
     7741 cgcccatgta gacccgctgg cgctggatct cgccggtctc cttgatcagg agctcgacgt
     7801 tgacgtacag cggccgcgaa tacgtcaggt ccttcgtccg gcactcctcc tcggagtact
     7861 tgggctcgcc gaactcgtag tcgaggaact ggagctccat caccttggcg gtgaagtcct
     7921 tgatcgggct gatctcgtcg aagagctccc ggagaccctt ctcgatgaac cactggaacg
     7981 agtccagctg gagttcgatc aggttcggga tcggcaggac ttcagggatg cgggcgtagc
     8041 gcgtacgcgc cagcgccaca gccgtccggt tcgacgactc agcagacagc atgaaacacc
     8101 actccttgac ggaggcttgg agaaaaggaa agggccgcgc tcacagaagg agtaggatac
     8161 cgcgatttcg tcgagtgtca aac
//
LOCUS       DATAIW010000245         6937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_244445, whole genome shotgun sequence.
ACCESSION   DATAIW010000245 DATAIW010000000
VERSION     DATAIW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244445"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1014)
                     /locus_tag="VHM48_07660"
     CDS             complement(16..1014)
                     /locus_tag="VHM48_07660"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2755322.1"
                     /translation="MKIGIDLGTANVLVYVKGKGVVITEPSVVAVSDDNRIVAVGEEA
                     REMIGRTPGNITAIRPMKDGVIADYVITEAMLRFFINKATKGHIGFSRPEVMISVPAG
                     VTSVEKRAVRDAALKAGAKEAYLIEEPLAAAIGANVPISGPSGNMVIDIGGGTSEIAV
                     IALGGIVVSTSLRVGGNKFDEHIATYIRKKYNLMIGERTAEEVKIQIGTALPLERELQ
                     MEVRGRDLIAGLPRTIPITSSEVMEAIEAPLQQLVAAVRTVLEQTPPELSSDIIDKGM
                     VMSGGGSLLRNIDKLLTQVTGVPCHVAENSLNCVAIGTGLALEHFDFFKKSLVSQV"
     gene            complement(1011..2357)
                     /gene="murA"
                     /locus_tag="VHM48_07665"
     CDS             complement(1011..2357)
                     /gene="murA"
                     /locus_tag="VHM48_07665"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX2755323.1"
                     /translation="MADARPFHWEFTPEPVTREVFRIEGGVPLRGEVPISGSKNAALK
                     MLAAATLTGERCRFTNVPEIEDVRVMTETLRDLGVVVDHPAPNTYEVASGDVDWLFVP
                     LEAAAKMRASFILLGPLLSRFGRVIISNPGGDRIGRRPVDLHIEAMRTLGAQIDYRNG
                     YFFATAPGRLRGTEVRFPFVSVMGTENAMLAATLADGHTVIRPAAQEPEVDDLILFLQ
                     KMGADVERTFPDTIEIQGKRRLRGAEHHVVPDRIEAGTFAVAGAVTGGQVTLTGAPCD
                     QMTALLDVFERVGVEARCDLDTITIDGRNVPEGGFRAVDIVTSPYPGLATDIQPPTCV
                     LLTQARGTSTVHEAIFEDRLEWLTELNRMGARIEIKDNQHATIHGRTPLHGADAEIGD
                     LRAGASLILAALAADGESTIHGAHHVQRGYENIERKFADLGARIARLEEGTVSTDS"
     gene            complement(2364..2792)
                     /locus_tag="VHM48_07670"
     CDS             complement(2364..2792)
                     /locus_tag="VHM48_07670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX2755324.1"
                     /translation="MPLQLEIVTPEKLAYRDEVDSVQLPGSEGELGVLPHHAPLITTL
                     GAGELRLRKGNDEESFAIVGGFLQVLPDKVVVMAETADMASEIDLEKAQEARRRAEQM
                     LEGGHVEGADLAAARASLQAALLRIRVAERRHREGPRRRG"
     gene            complement(2796..4208)
                     /gene="atpD"
                     /locus_tag="VHM48_07675"
     CDS             complement(2796..4208)
                     /gene="atpD"
                     /locus_tag="VHM48_07675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2755325.1"
                     /translation="MASAAPAATGQVIQITGPVVDIEFPAGRLPAIYNAVAIQRKGMP
                     DLVCEVQQHLGNNWVRAVAMTTTDGLARGVDVLDTGGPISVPVGEVTLGRVFDVLGNA
                     IDGKEPVDRSKTLPIHRSAPAFDQQTTEVEVFETGLKIIDLICPFKKGGKIGIFGGAG
                     VGKTVIIQELIRNVAQEHAGVSVFAGVGERSREGNDLIKEMTESGVIEKTAFVFGQMN
                     EPPGARQRVGLTGLTMAEYFRDEEHRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGY
                     QPNLATEMAALQERITSTKDGSITSLQAVFVPADDYTDPAPATTFAHLDSTIRLERSI
                     AELGIYPAVDPLTSTSRVLDPLVVGQEHYDVAQETKRVLQRYRDLQDIIAILGIDELS
                     AEDKAVVGRARRLQRYASQPFFVAEVFTGRPGAYVSIKDTVASFKEILDGKVDDLPEQ
                     AFFLAGTIEDVRKNAEQLAG"
     gene            complement(4233..5084)
                     /gene="atpG"
                     /locus_tag="VHM48_07680"
     CDS             complement(4233..5084)
                     /gene="atpG"
                     /locus_tag="VHM48_07680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX2755326.1"
                     /translation="MASQRDIRRRIQSSKNIKQITRAMQFVAASKLKRAQEATLASRP
                     YAEKLDEVLADLAIVLDGDDHPLLVRREGGKRLIVLITTDRGLAGGLNTNTIRFAAQQ
                     ITEKGGDLTMVTVGRKGRDAMRRARVPLAAHFEGFGDRPTFADVLPLARLITDDYLAG
                     TYARVDIVYSHFVSTLTQKASMVELLPVVPPADTTGIPGNQFIFEPNPAAVLEQLVPR
                     YVATRLFQAVLEGKASEESSRMVAMKNATENAEELIEDLTLAYNKVRQSNITREMIEI
                     ASGAKAG"
     gene            complement(5274..6809)
                     /gene="atpA"
                     /locus_tag="VHM48_07685"
     CDS             complement(5274..6809)
                     /gene="atpA"
                     /locus_tag="VHM48_07685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX2755327.1"
                     /translation="MAIRSDEITSIIKSSIDSFDAATETRSVGTVVEVGDGIAQIYGL
                     DGALASEMLEFPGGVMGMALNLEEETVGAVILGDPRAIKEGDTVKTTGRVVEVPVGQG
                     LLGRVVDPLGRPLDDKGPIAATKTRPVERIAPGVIVRKSVDTPVQTGIKAIDALIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGKGLVCIYVAIGQKLSTVAQTVAILENNGAM
                     GHTIVVVAGAEDPAPLQYLAPYAGVTMGEEVMENGVEVEGEGLVKDALCVYDDLSKHA
                     WAYREMSLLLRRPPGREAYPGDVFYLHSRLLERAARMNDDNGGGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIFLETDLFNAGQRPALNIGISVSRVGSAAQTKAMKKVAAPLK
                     LDLAQYRSLAAFAQFASDLDKATRDQLTRGEKLSEIVKQPQYQPLAVEKQVAILYAAT
                     KGKLDDVPTNRVKDFETGLYRFLETDQPQILEELGKTQALTDELSAALDKALDDFRKQ
                     FNA"
BASE COUNT          980 a         2264 c         2493 g         1200 t
ORIGIN      
        1 cggccatcga acctcctaga cctgggacac gagcgacttc ttgaagaagt cgaagtgctc
       61 gagggccagc cccgtcccga tggcgacgca gttgagggag ttctccgcca cgtggcaggg
      121 aacgccggtc acctgggtca gaagcttgtc gatgttgcgc agcaacgagc cgccgcccga
      181 catgaccatg cccttgtcga tgatgtccga gctcagctcg ggcggggtct gctcgaggac
      241 cgtccggaca gccgcgacga gctgctggag cggagcctcg atggcctcca tcacctcgga
      301 gctggtgatc gggatcgtcc gcggcaggcc ggcgatcagg tctcgcccac ggacctccat
      361 ctggagctcg cgctcgagcg gcagggcggt cccgatctgg atcttcacct cttcggccgt
      421 ccgctcgccg atcatcaggt tgtacttctt gcggatgtac gtcgcgatgt gctcgtcgaa
      481 cttgttgccg ccgacccgga gcgaggtcga gacgacgatc ccgccgaggg cgatgaccgc
      541 gatctcgctc gtcccgccgc cgatgtcgat gaccatgttg cccgacgggc cgctgatcgg
      601 gacgttggcg ccgatcgcgg ccgccagcgg ctcctcgatg aggtaggcct ccttggcgcc
      661 ggccttgagg gcggcgtcgc ggaccgcccg cttctcgacc gaggtcaccc cggccgggac
      721 gctgatcatc acctcgggcc gggagaagcc gatgtggccc ttcgtcgcct tgttgatgaa
      781 gaagcggagc atcgcctccg tgatgacgta gtcggcgatg accccgtcct tcatcgggcg
      841 gatggcggtg atgttgccgg gggtgcgccc gatcatctcg cgggcttcct cgcccaccgc
      901 gacgatccgg ttgtcgtcgg acaccgcgac cacggatggt tccgtgatca cgactccctt
      961 gcccttgacg tagacgagga cgttggccgt gccgaggtcg atgccgatct tcatgagtct
     1021 gtggaaacgg tcccttcttc gaggcgcgcg attctggcgc ccaggtctgc gaacttgcgt
     1081 tcgatgttct catacccccg ctggacgtgg tgtgcgccgt gaatcgtcga ctcgccatcg
     1141 gcggcgaggg cggcgaggat gagcgatgca ccggcccgca ggtccccgat ctcggcgtcg
     1201 gccccgtgga ggggcgtccg gccgtggatc gtggcgtgct ggttgtcctt gatctcgatc
     1261 cgggcgccca tccggttgag ctctgtcagc cattcgaggc gatcctcgaa gatcgcctcg
     1321 tggaccgtcg aggtgccccg cgcctgggtc aggaggacgc aggtcggcgg ctggatatct
     1381 gtcgcgagac cgggataggg ggaggtcacg atgtcgacgg cacggaaccc accctcgggc
     1441 acgttgcggc cgtcgatcgt gatcgtgtcg aggtcgcacc gggcttcgac gccgacgcgc
     1501 tcgaagacgt cgagcagggc ggtcatctgg tcgcacggtg caccggtcag ggtgacctgg
     1561 ccgccggtga cggcgcccgc gacggcgaac gtcccggcct cgatccgatc gggcacgacg
     1621 tggtgctcgg cgccgcgcag gcggcgcttg ccctggatct cgatcgtgtc ggggaatgtc
     1681 cgctcgacgt cggcgcccat cttctggagg aacaggatca ggtcgtcgac ctccggttcc
     1741 tgggccgccg gccggatcac cgtgtggccg tcggcgaggg tcgccgccag catcgcgttc
     1801 tcggtgccca tgaccgacac gaacgggaag cggacctccg tgccgcggag ccgccccggg
     1861 gccgtcgcga agaagtagcc gttgcggtag tcgatctgcg cgccgagcgt ccgcatcgcc
     1921 tcgatgtgga ggtcgaccgg acggcggccg atccggtcac cgccggggtt gctgatgatc
     1981 acccgcccga accggctgag gagcgggccg aggaggatga acgacgcacg catcttggcc
     2041 gccgcctcga gcggcacgaa cagccagtcg acgtcgcccg aggcgacctc gtaggtgttc
     2101 ggtgccgggt ggtcgaccac gacgccgaga tcgcgcaggg tctcggtcat gacccggaca
     2161 tcctcgatct cgggaacgtt ggtgaaccgg cagcgctcgc cggtcagggt cgccgcggcg
     2221 agcatcttga gggccgcgtt cttggagccg ctgatcggca cctcgccgcg caggggcacg
     2281 ccgccctcga tccggaagac ctcgcgggtc accggctctg gggtgaactc ccagtggaac
     2341 ggacgagcgt ctgccatgga ccgctagccg cgccggcgcg ggccctcgcg atggcgacgc
     2401 tcggcgacgc gtatccggag cagggccgcc tggagcgacg ctcgggcggc ggcgaggtcg
     2461 gcgccttcga cgtggccgcc ctcgagcatc tgctcggccc ggcggcgcgc ctcctgggcc
     2521 ttctcgaggt cgatctccga cgccatgtcg gcggtctcgg ccatcaccac gaccttgtcc
     2581 ggcagcacct ggaggaagcc gccgacgatc gcgaacgact cctcgtcgtt gccctttcgc
     2641 agccgcagct cgcccgcccc gagcgtcgtg atcagcggcg cgtggtgggg caggacgccg
     2701 agctcgccct cgctgccggg cagctggacg ctgtcgacct cgtcgcggta ggcgagcttc
     2761 tcgggcgtga cgatctcgag ctggaggggc atggctcagc ccgccagctg ctcggcgttc
     2821 ttgcggacgt cctcgatcgt gccggcgagg aagaacgcct gctccggcag gtcgtcgacc
     2881 ttgccgtcga ggatctcctt gaagctcgcg acggtgtcct tgatcgagac gtacgcaccc
     2941 gggcggccgg tgaagacctc ggccacgaag aacggctggc tcgcatagcg ctggaggcgg
     3001 cgggctcggc cgacgaccgc cttgtcctcg gccgacagct cgtcgatccc gaggatcgcg
     3061 atgatgtcct ggagatcgcg gtagcgctgg aggacgcgct tggtctcctg ggcgacgtcg
     3121 taatgctcct ggccgacgac gagcgggtcg aggacgcgcg aggtcgaggt cagcgggtcg
     3181 acggccgggt agatcccgag ctcggcgatc gagcgctcga gacggatcgt cgagtcgaga
     3241 tgggcgaagg tcgtcgccgg agccgggtcg gtgtagtcgt ccgccgggac gaagaccgcc
     3301 tggagcgagg tgatcgagcc gtctttcgtc gaggtgatcc gctcctgcag ggcggccatc
     3361 tcggtcgcga ggttcggctg gtagccgacc gcggagggca tccggccgag cagtgcggac
     3421 acttcggagc ccgcctgggt gaagcggaag atgttgtcga tgaagaggag gacgtcgcga
     3481 tgctcctcgt cgcggaagta ttcggccatc gtcaggccgg tcaggccgac ccgctggcgg
     3541 gcgccgggcg gctcgttcat ctggccgaac acgaaggccg tcttctcgat gacccccgat
     3601 tcagtcatct ccttgatcag gtcgttgccc tcgcgtgagc gctcgccgac gccggcgaac
     3661 accgacacgc cggcgtgctc ctgggcgacg ttccggatca gctcctggat gatgaccgtc
     3721 ttgccgacgc cggcgccacc gaagatcccg atcttgccgc ccttcttgaa tgggcagatc
     3781 aggtcgatga tcttgaggcc ggtctcgaag acctcgacct cggtcgtctg ctggtcgaag
     3841 gccggggcgc tgcggtggat cggcagggtc ttgctgcggt cgaccggctc cttgccgtcg
     3901 atcgcgttgc cgaggacgtc gaacacccgc ccgagcgtca cttcgccgac cgggaccgag
     3961 atcgggccgc cggtgtcgag gacgtcgacg ccgcgggcaa gcccgtcggt cgtggtcatc
     4021 gcgaccgccc ggacccagtt gttgccgaga tgctgctgga cctcgcagac gaggtcgggc
     4081 atgcccttcc gctggatcgc gacggcgttg tagatcgccg gcaggcggcc ggccgggaac
     4141 tcgatgtcga cgacgggacc ggtgatctgg atcacctggc cggtcgcggc gggtgcggcg
     4201 ctcgccatgg ggttcgttgc ctcctgctcg cgtcagccgg ccttggcgcc ggacgcgatc
     4261 tcgatcattt cgcgggtgat gtttgactgg cggaccttgt tgtaggcgag ggtcagatcc
     4321 tcgatcagct cctcggcatt ctcggtcgcg ttcttcatcg cgaccatccg gctcgattcc
     4381 tcgctcgcct tgccctcgag gacggcctgg aacaggcggg tcgcgacgta gcgcggcacg
     4441 agctgttcga gcacggccgc gggattcggc tcgaagatga actggttgcc tggaatgccg
     4501 gtcgtatccg cgggcggaac gacgggcagg agctcgacca tcgacgcctt ctgggtcagg
     4561 gtcgagacga agtggctgta gacgatgtcg acccgggcat aggttccggc gaggtagtcg
     4621 tcggtgatga gccgggcgag gggcagcacg tcggcgaagg tcggccggtc gccgaacccc
     4681 tcgaagtggg ccgccagcgg gactcgggcg cggcgcatcg cgtcgcgccc cttgcggccg
     4741 acggtgacca tcgtcaggtc gccgcccttc tcggtgatct gctgggcggc gaagcggatc
     4801 gtgttcgtgt tcaggccgcc ggcgaggcca cggtccgtcg tgatcagcac gatcaggcgc
     4861 ttgccgccct cgcgccggac gagcagcgga tggtcgtcgc cgtcgaggac gatcgcgagg
     4921 tcggcgagga cttcgtcgag cttctcggcg tagggccgtg aggcgagggt ggcctcctgg
     4981 gcgcgcttga gcttcgaggc ggccacgaac tgcatcgccc gggtgatctg cttgatgttc
     5041 ttcgacgact ggatgcgtcg acgaatatcg cgctggctag ccacggctcg cctccacgca
     5101 cgggaccggc cgatcggtgt ggctgatcaa tccggccaca accacgctgg gttgtcgtcc
     5161 cggatcgccg accaacgaca tcctgagcgg ccggccgcgc tcacctgcgc ccgaatcgat
     5221 cagcggggct gatcgggtcg ggggcgcagc gtgggccgcc atggcgtcga tcatcaggcg
     5281 ttgaactgct tgcggaagtc gtcgagggcc ttgtcgaggg ccgccgacag ctcgtcggtc
     5341 agggcctgcg tcttgccgag ctcctcgagg atctggggct ggtcggtctc gaggaagcga
     5401 tacaagccgg tctcgaagtc cttgacccgg ttggtcggga cgtcgtcgag cttgcccttc
     5461 gtggccgcat acaggatggc gacctgcttc tcgaccgcga gcggctggta ctgcggctgc
     5521 ttgacgatct cggacagctt ctcgccccgg gtcagctggt cgcgggtggc cttgtccagg
     5581 tcggacgcga actgggcgaa ggccgccagg ctgcggtact gggcgagatc cagcttgagg
     5641 ggcgccgcga ccttcttcat cgccttggtc tgggcagcgg agccgacgcg cgacaccgag
     5701 atgccgatgt tcagggcggg ccgctggccg gcgttgaaca ggtcggtctc gaggaagatc
     5761 tggccgtcgg tgatcgagat gacattcgtc gggatgtagg ccgagacgtc gccggcctgg
     5821 gtctcgatga tcggcagggc cgtcagcgag ccgccgccgt tgtcgtcgtt catccgggcg
     5881 gcccgctcga gcagccggga atggaggtag aagacgtcgc ccggatacgc ctcgcggccg
     5941 ggcgggcggc ggaggagcag ggacatctcg cgataggccc aggcgtgctt gctgaggtcg
     6001 tcgtagacgc acagggcgtc cttgacgagg ccctcgccct cgacctcgac accgttctcc
     6061 atgacctcct cgcccatcgt cacgccggcg tacggggcga gatactggag cggcgccgga
     6121 tcctcggcgc cggcgacgac gacgatcgtg tggcccatcg cgccgttgtt ctcgagaatg
     6181 gccaccgtct gggcgaccgt cgagagcttc tggccgatcg ccacgtagat gcagacgagg
     6241 cccttgccct tctggttgat gatcgtgtcg atcgcgatcg cggtcttgcc cgtctggcgg
     6301 tcgccgatga tcagctcgcg ctggccgcgg ccgatcggga tcagggcgtc gatcgccttg
     6361 ataccggtct ggaccggggt gtcgaccgac ttgcggacga tgacgccggg ggcgatccgc
     6421 tcgaccgggc gggtcttcgt cgcggcgatc gggcccttgt catcgagcgg tcgaccgagc
     6481 gggtcgacga cccgaccgag cagcccctgg ccgacgggga cctcgacgac gcggccggtg
     6541 gtcttgacgg tgtcgccttc cttgatcgcc cggggatcgc cgaggatgac cgcgccgacc
     6601 gtctcctcct cgaggttgag ggccatgccc atgaccccac ccgggaactc gagcatctcc
     6661 gacgcgaggg cgccgtcgag cccgtagatc tgggcgatgc cgtcaccgac ctcgacgacc
     6721 gttccgacgc tgcgcgtctc ggtcgccgcg tcgaacgagt cgatcgacga cttgatgatg
     6781 ctggtgatct cgtcggaacg aatggccatg ttcgggttct cctacagggc gcccgaggcg
     6841 agctggttcc gtagccgctc gaggcggccc cggacgctcc cgtcgatgag tcggtcgccg
     6901 acgcggacga tcagcccgcc gagcaggtct gcgtcga
//
LOCUS       DATAIW010000246         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_214561, whole genome shotgun sequence.
ACCESSION   DATAIW010000246 DATAIW010000000
VERSION     DATAIW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214561"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VHM48_07690"
     CDS             complement(<1..1229)
                     /locus_tag="VHM48_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX2755328.1"
                     /translation="MYVVILAGGGGTRLWPLSRQERPKPFLPLVDDRSLLQHTVDRIA
                     PLVGSDDVFCVADRRYGQLIRDQVPDVGLIVEPAGRNTAAAIALAGMVIDRPDDEVMI
                     VLPADHWIADEARFRDVLRVAAERLATGSFEIEQPLVTLGVRPTFPSTDYGYLRPDTM
                     RGTKIDGVPANPLLGFEEKPTEARARELINLPGIAWNAGMFAWQRSAIRAALEKYTPL
                     PMLIDQAVGSELALANAYERIAPISIDRAVMESAAADHRVVMGAMDVGWSDLGSWTAL
                     LAAIAGDRVAGATGRVVQPGETIQLGPDDLVVRPADGHLTVEVGPTVAGGSGGTIVAD
                     GVWAHLAGARHLIDEVRGLLERVDHHSGERDRGRRVPPGRLDDQPDVRDLVADQLTVA
                     PVGDAEDVVRPDERRDSV"
     gene            complement(1449..2006)
                     /locus_tag="VHM48_07695"
     CDS             complement(1449..2006)
                     /locus_tag="VHM48_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2755329.1"
                     /translation="MAATELQLGPRIRSLRQARQVTLRELAERAGVTESFLSQVEREV
                     TSPSIASVQRIARALDLGIAELFVDEPPIGRIVRRAERRRIVYPGLDAVDEFLTAGRD
                     GRLQVILTTLEPGGGTGDEAYTHESDEEVVIVLEGRLELWVGDEFHLLADGDSITYSS
                     RQPHRNRNPGPGRAVILFCATPPSF"
     gene            2290..3435
                     /locus_tag="VHM48_07700"
     CDS             2290..3435
                     /locus_tag="VHM48_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755330.1"
                     /translation="MGARPAGATGTAHPDVEMVGVTKRFGDVVAVDHMNLAIPSASFT
                     SLLGPSGCGKTTTLRMIAGFEQPTEGEILLAGKPIAGVPPYQRNVNTVFQHYALFPHM
                     DVAQNVGYGLRQRKVGKAEEKRRVAEALELVRLVGYGKRRTWEMSGGQQQRVALARAL
                     VNHPTVLLLDEPLGALDLKLRKEMQLELKALQQEVGITFVYVTHDQEEALTMSDMIVV
                     MSEGVIQQQGGPEELYEHPVNRFVADFIGTSNPIPGRIVSVDTATRRAVIDSDRGLRL
                     HGRVTALDAMPAAGDQVVVAIRPERLRIEPAETAVTEDGSNAVEGRVRQGTYLGDQTE
                     YRVDTAEAGEMIVRRQNAAGANNGPGLGPGDRVVISWREEANLVLIS"
BASE COUNT          560 a         1251 c         1304 g          515 t
ORIGIN      
        1 accgaatcgc gccgctcgtc ggggcggacg acgtcttctg cgtcgccgac cggcgctacg
       61 gtcagctgat ccgcgaccag gtcccggacg tcgggttgat cgtcgagccg gccgggcgga
      121 acacggcggc cgcgatcgcg ctcgccggaa tggtgatcga cccgctcgag caggccccgg
      181 acctcgtcga tgaggtggcg ggcgccggcg aggtgggccc acacgccgtc ggcgacgatc
      241 gtaccgcccg agccgccggc gacggtcggt cccacctcca cggtaaggtg cccgtcggcc
      301 ggccggacga ccaggtcgtc gggaccgagc tggatcgtct caccgggctg gaccacccga
      361 cccgtggccc cggcaacacg gtcgccggcg atcgccgcga ggagcgcggt ccagctgccg
      421 agatcgctcc aaccgacatc catcgcgccc atcacgaccc gatggtcggc ggcggcgctt
      481 tccatgacgg ctcgatcgat cgagatcggg gcgatccgct cgtaggcgtt ggcgagggcg
      541 agctccgagc cgaccgcctg gtcgatgagc atcggcagcg gggtgtactt ctcgagcgcg
      601 gcccggatcg cgctgcgctg ccaggcgaac atgccggcgt tccaggcgat gccgggcagg
      661 ttgatcagct cgcgggcccg cgcctcggtc ggcttctcct cgaacccgag cagcgggttg
      721 gccgggacgc cgtcgatctt cgtcccgcgc atcgtgtcgg ggcggagata gccgtagtct
      781 gtcgacggaa aggtcggtcg gacgccgagc gtcacgagcg gctgctcgat ctcgaacgag
      841 ccggtcgcga gccgttcggc cgcgacgcgc aggacgtccc ggaagcgggc ctcgtccgcg
      901 atccagtggt cagccggcag gacgatcatg acctcgtcgt cgggacggtc gatcaccatt
      961 ccggcgagcg cgatcgcggc cgccgtgttc cgcccggccg gctcgacgat caacccgacg
     1021 tccgggacct ggtcgcggat cagctggccg tagcgccggt cggcgacgca gaagacgtcg
     1081 tccgacccga cgagcggggc gatgcgatcg accgtgtgct ggagcaacga ccgatcgtcg
     1141 acgagcggca ggaagggctt cgggcgctct tgccggctga ggggccacaa ccgggtcccg
     1201 cctccgccgg ccaggatgac gacgtacatg gccgcccatg ataccggtgg cacgaggccg
     1261 gccggcgggc actcggagtc gtcgcgctcc agccccgact caccccgcga gtcaagatcg
     1321 gcgtcagcga gcgtggatgc gtcgtgaatc ggacggagcc gtgcgtgctg atgccggatt
     1381 tgactcaggg cgtgagtcag ccggtcgagg tccgcggcga ccgcacgatt cgagcctgaa
     1441 gcggtgggtc agaagctcgg cggcgtggcg cagaagagga tgacggcccg gcccggtccc
     1501 ggattgcggt tgcggtgcgg ctggcgggac gagtaggtga tcgagtcgcc gtcggcgagg
     1561 aggtggaact cgtcgccgac ccacagctcc aggcggccct cgaggacgat cacgacctcc
     1621 tcgtccgact catgggtgta cgcctcgtcg cccgtcccgc cgcctggctc gagcgtcgtc
     1681 aggatcacct ggaggcggcc gtcacgaccc gcggtcagga actcgtcgac ggcatcgagg
     1741 cccggataca cgatccgccg ccgttcggcg cggcggacga tccgtccgat cggtggctcg
     1801 tcgacgaaca gctcggcgat gccgaggtcg agggcgcggg cgatccgctg gaccgacgcg
     1861 atcgatggac tcgtcacctc gcgctcgacc tgggagagga agctctcggt cacgccggct
     1921 cgttcggcca gctcgcggag ggtcacctgg cgggcctgcc ggagcgaccg gatccggggc
     1981 ccgagctgaa gctccgtggc tgccatcgtc ctgcaaccct ccgatcgtcc ggggcggcga
     2041 ccaacgaggc tgaagtgtag cttcagtgat tcgtgcgact tcccagcgca tgggtcgaac
     2101 cggattcgaa tatcgtgcgc ccactgcgtt gacgctgccg aactgcgccg tctaccatcc
     2161 ccgcacgctg tagcatcgct acaccgacgc ccggttgccg cacccattcc gctgcgcaga
     2221 gaggggccag tgaccgttcc cgcgaccgcc gccgatcctg gaccatcccc tgccgccggg
     2281 tcggccgcca tgggcgcgcg ccccgccggg gcgacgggca cggcccaccc ggacgtcgag
     2341 atggtcggtg tcacgaagcg attcggcgat gtcgtcgccg tcgaccacat gaatctcgcg
     2401 atcccgtccg cgtccttcac cagcctgctc ggcccgtccg gctgtggcaa gacgacgacg
     2461 ctccggatga tcgccgggtt cgagcagccg accgagggcg agatcctcct cgccggcaag
     2521 cccatcgccg gcgtgccgcc gtaccagcgc aacgtcaaca cggtcttcca gcactacgcg
     2581 ctcttccccc acatggacgt tgcccagaat gtcggctacg ggctccgcca gcggaaggtc
     2641 ggcaaggccg aggagaagcg ccgcgtcgcg gaggcgctcg agctcgtccg gctcgtgggc
     2701 tacggcaagc gccggacgtg ggagatgtcg ggcggccagc agcagcgggt cgccctcgcc
     2761 cgggcgctcg tgaaccatcc gaccgtgctc cttctcgacg agccgctcgg cgcgctcgac
     2821 ctgaagctcc gcaaggagat gcagctcgag ctcaaggccc tccagcagga ggtcgggatc
     2881 acgttcgtct acgtgaccca cgaccaggag gaagccctga cgatgagcga catgatcgtc
     2941 gtgatgagcg agggcgtgat ccagcagcag ggcggccccg aggagctcta cgagcacccc
     3001 gtcaaccgct tcgtcgccga tttcatcgga acgtcgaacc cgatccccgg tcgaatcgtg
     3061 agtgtcgaca cggccacacg gcgggcggtc atcgactcgg atcgtggcct ccggctgcat
     3121 ggccgggtca ctgcgctcga cgccatgccg gccgcaggcg accaggtcgt ggtcgcgatc
     3181 cggcccgagc gactgcggat cgagcccgcc gagacggccg tgaccgagga cggctcgaat
     3241 gccgtcgagg gccgcgtccg gcagggcaca tatctcggcg accagaccga atatcgggtc
     3301 gacacggcgg aggcgggcga gatgatcgtc cgccgccaga acgcagcggg ggccaacaat
     3361 ggaccagggc tcggaccggg cgaccgggtc gtgatctcct ggcgcgagga ggccaatctg
     3421 gtcctcatca gctgagggct cccgaccgcg caccctgcgc ggtcggagag tcggcgtggc
     3481 ggcggggtga gccgtcttcg ggacggcagg aggagacatc gtggcagacc aggatctcga
     3541 gcacatcctg cggcggatgt cgcaggcacg ggtgaaccgc cgggggttca tggcgggcgg
     3601 ggccatggcc gggatggcgg cgttcatcgc
//
LOCUS       DATAIW010000247         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_274432, whole genome shotgun sequence.
ACCESSION   DATAIW010000247 DATAIW010000000
VERSION     DATAIW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274432"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>4026
                     /locus_tag="VHM48_07705"
     CDS             271..>4026
                     /locus_tag="VHM48_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2755331.1"
                     /translation="MPASPVRSRRSSAVSRFAAALGAAALLGSLIPAAIAAPIVASGS
                     VAQAASTPAAASHTPDPTSVTIAGNLQPALGCPGEWQADCAATHLLYDPNDQVWQGSF
                     DVPAGSWEYKSALNDSWTESYGKSDGNNVTLPLGAPRTVKFYYDHKTHWVTDNVNSVI
                     AIAAGSFQDDLGCSGEWQENCLRSWLQDPDGDGVYTFVTTAIPAGSYATKVTINESWS
                     ENYGQGGVPGGSDIAFTVPENGAKVTFRYVAATHVLTVSAGHGHDGNVEWDGLRHDSR
                     DTTYRTPGGAVPAGTAVTIRFRTFHGDATGVKVRFYSLRLAGQQVVPMSIAAGGAPCG
                     ADVPAGETCDFWSVTMPQAIASQPDNLWYRFIVTDGTDTDFYGDDTPALDGGLGAPSD
                     DVIDQSWALMQFVPGFTAPSWAKDAVIYQIFPDRFRNGRKDNDPKTGDIRYDDPVIKL
                     PWSVKPEGYCRNYADGATNCPWRFDTTPPADSPTKESPRGRDYFGGDLKGVDQQLAYL
                     DVLGVTAIYFNPIFDAGSNHSYDTQDYTKVDPYFGTQKDFDNLIKHAKSLGIRVILDG
                     VFNHMSSDSPLFDRYGHYATVGACESTASPYRSWFVFTNVAAGTGTCAGSAGPKSASY
                     EGWFGFDSIPVLRKDQAAVQAYFLTAPDSIAKRWLQSGASGWRLDVSGDASFPNGYWE
                     SFRSVVKATNPQALTISETWQKDSTLLRMLRGDRLDTTMNYRLRDAVLALLAPQAFDS
                     KGFADSGHQISVSDFADRLASVREDYADATYYSLMNLLDSHDTERLLWTLTPGVATTA
                     AKELNAANVAAGKLRVKLASLIQFTVPGAPTIYYGDEVGMTGADDPDDRRTYPWADLG
                     GHPDTTLLAHYTALANLRTAVPALRDGDFRVLLADDPAETVAYGRKTGSQVAILVLNR
                     SGSTRLIDIPVGGYVPDGIALARRFGVGVAATGSVTVAGGGVTVSLPPMSGLVLASGT
                     VDLTPPPAPTNLHVTGEAANQLSIAWTASAGATGYDVWVSPLSGGGYIKANTAPVSGT
                     TFTIDGLDNARTYYIVVRALDAAGNASAPSNEAHGLPHLVIGWANLQWPPTLIHVVSA
                     TNRTDNVYGQVWIDGATNQPGATPSLTAQLGFGPDNSDPDGNPAWQWVEAAFNTDAGN
                     NDEFVASLLPEATGTFDYAYRYSVTAGRDWVYADLDGIGNGYSTGQAGDLTVIPSADT
                     TAPAIPTGLHVGSASPAGIELAWDAVGGDASLYGYEVLRGDASGGPFT"
BASE COUNT          653 a         1510 c         1289 g          574 t
ORIGIN      
        1 gagtccgggt cgcccgacgc tgagggcgcg ccgccggcgc ctgcatcgcc gccccaccgc
       61 aactaaacgc ggactaaacg gccaccggcc gttgacaccg gcgcagcccg gacgcatcct
      121 gaccgggcgg cccgcttaac cggtttagta gcacgcgatc acgtgccggc cgctcggcac
      181 cgccagagcc gtgcgccaga tcccgacgca cgaacgcgcc atccgcccag cctcgccagt
      241 gctcggtcag gtccatggag gttccgttcg atgcccgctt cgcccgttcg ctcccgtcgt
      301 tcgtcggcgg tctcccggtt cgccgccgct ctcggggcgg ccgccctgct gggctccctg
      361 atcccggcgg cgatcgcggc tccgatcgtc gcgtcgggct cggtcgccca ggccgcgtcg
      421 actcccgcgg cggcgtcgca cacgccggac ccaaccagcg tgacgattgc gggaaatctc
      481 cagccggcgc tcggctgccc gggcgagtgg caggccgatt gcgccgcgac ccacctcctc
      541 tacgacccga acgaccaggt ctggcagggc tcgttcgacg tccccgccgg ttcgtgggag
      601 tacaagtcag cgctaaacga ctcctggacc gagagctacg ggaagagcga cggaaacaac
      661 gtcacgctgc cgctcggcgc gccgcgaacc gtgaagttct actacgacca caagacccac
      721 tgggtgaccg acaacgtcaa ctcggtcatc gcgatcgcgg ccggcagctt ccaggacgac
      781 ctcggctgta gcggtgagtg gcaggagaat tgcctccgct cgtggctcca ggatccagac
      841 ggcgacggcg tctacacgtt cgtcacgacc gcgatccccg ccggcagcta cgcgacgaag
      901 gtcacgatca acgagagctg gtcggagaac tacggccagg gtggcgttcc cggcggcagc
      961 gacatcgcgt tcaccgtccc ggagaacggc gccaaggtca cgttccgcta cgtcgcggcg
     1021 acccacgtcc tgaccgtcag cgccggccat ggccatgacg gcaacgtcga gtgggacggc
     1081 ctccgccacg actcacgcga cacgacctac cggacgcccg gtggcgcggt cccggccgga
     1141 accgcggtga cgatccgctt caggacgttc cacggggacg cgaccggcgt caaggtccgc
     1201 ttctacagcc tcaggctggc gggccagcag gtcgtcccga tgtcgatcgc ggccggcggc
     1261 gccccgtgcg gcgcggacgt tcccgcgggc gagacgtgcg acttctggag cgtgacgatg
     1321 ccccaggcga tcgccagcca gccggacaac ctgtggtacc ggttcatcgt gaccgacggc
     1381 accgacacgg atttctacgg cgacgacacg cccgccctcg acggcgggct cggcgccccg
     1441 agtgacgacg tcatcgatca gagctgggct ctcatgcagt tcgtccccgg cttcaccgcg
     1501 ccgagttggg cgaaggacgc ggtcatctac cagatcttcc cggaccgctt ccgcaacggc
     1561 cgcaaggaca acgacccgaa gaccggcgat atccgctacg acgacccggt catcaagctc
     1621 ccgtggtcgg tgaaacccga gggctactgc aggaactacg ccgacggcgc gacgaactgc
     1681 ccatggcggt tcgacacgac cccgccggcc gacagcccga ccaaggagag cccacgcggc
     1741 cgggactact tcggcggcga cctcaagggc gtcgaccagc agctcgccta cctcgatgtg
     1801 ctcggcgtca ccgcgatcta cttcaacccg atcttcgacg ccggctcgaa ccattcgtac
     1861 gacacccagg actacacgaa ggtcgacccg tacttcggaa cgcagaagga cttcgacaac
     1921 ctgatcaagc acgccaagtc gctcgggatc cgggtgatcc tcgacggcgt gttcaaccac
     1981 atgagctcgg acagcccgct gttcgaccgg tacgggcatt acgcgaccgt cggggcttgc
     2041 gagtcgacgg catcgccgta tcggtcgtgg ttcgtcttca cgaacgtcgc ggccgggacg
     2101 ggcacgtgcg ccggttcggc cggcccgaag tcggcgagct acgaaggctg gttcggcttc
     2161 gattcgatcc ccgtcctgcg caaggaccag gccgcggtcc aggcgtactt cctgaccgcg
     2221 cccgatagca tcgccaagcg ttggctccag tccggcgcgt ccggctggcg gctcgacgtg
     2281 tcgggcgacg cgtcgttccc gaacggctac tgggaatcgt tccggtcggt cgtcaaggcc
     2341 acgaacccgc aggcgctgac gatcagcgag acctggcaga aggactcgac gctgctccgg
     2401 atgctccgcg gcgaccgcct cgacacgacg atgaactacc gcctccgcga cgcggtcctg
     2461 gcgctgctcg ccccgcaggc cttcgactcg aagggcttcg ccgacagcgg ccaccagatc
     2521 agcgtcagcg acttcgccga ccgcctcgcc tcggtgcgcg aggattacgc cgacgcgacg
     2581 tactactcgc tgatgaacct cctcgacagc cacgacacag agcgcctcct ctggacgctc
     2641 acgccgggcg tcgcgacgac ggcggccaag gagctgaacg cggccaacgt cgcggccggc
     2701 aagctccgtg tcaagctcgc gtcgctcatc cagttcacgg tccccggcgc accgacgatc
     2761 tactacggcg acgaggtggg catgacgggc gccgacgacc ccgacgatcg gcggacctat
     2821 ccgtgggccg acctcggcgg ccatccggac acgaccctcc tcgcccacta caccgccctc
     2881 gccaacctcc gtacggccgt cccggcgctg cgcgacggcg atttccgggt gctcctcgcc
     2941 gacgatcccg cggaaaccgt ggcctacggc cggaagaccg gcagccaggt cgcgatcctc
     3001 gtcctcaacc gctccggttc cacccgcttg atcgacatcc cggtcggcgg ttacgtgccg
     3061 gatggcatcg cgctggcccg gcgcttcggc gtcggcgtcg ccgcgaccgg ctccgtcacg
     3121 gtggccggcg gcggcgtgac cgtgtccctg ccgccgatgt ccggcctcgt cctcgccagt
     3181 ggcacggtcg acctgacccc gccgccggcc ccgaccaacc tccacgtcac cggcgaggcg
     3241 gccaaccagc tgtcgatcgc gtggacggct tccgccggag ccacgggcta cgacgtctgg
     3301 gtcagcccgc tcagcggcgg cggctacatc aaggcgaaca ccgcgccggt gtccggcacg
     3361 acgttcacga tcgacggcct cgacaacgcc cggacctact acatcgtcgt gcgggcgctg
     3421 gacgccgccg gcaacgcgtc ggccccgtcg aacgaggcgc acggcctgcc ccatctcgtg
     3481 atcggctggg cgaacctcca gtggccgccc acgctgatcc atgtcgtcag cgcgaccaat
     3541 cgaaccgaca acgtctacgg ccaggtctgg atcgacggcg cgaccaatca gccgggcgcc
     3601 acgccgagcc tcaccgccca gctcgggttc gggccggaca actccgatcc cgacggcaac
     3661 ccggcgtggc agtgggtcga agcggcgttc aacaccgatg ccggcaacaa cgacgagttc
     3721 gtcgcctcgc tcctgcccga ggcaacgggc acgttcgatt acgcctaccg ctattcggtc
     3781 acggctggcc gcgactgggt ctacgccgac ctcgacggga tcggcaacgg ctactcgacc
     3841 ggacaggccg gcgatctgac cgtgatcccg agcgcggaca ccaccgcccc ggcgatcccg
     3901 acggggctcc atgtcgggtc ggcatcgccc gccggcatcg agctcgcctg ggatgccgtc
     3961 ggcggtgatg ccagcctcta cggctacgag gtcctccgcg gcgacgcttc gggcggtccg
     4021 ttcacc
//
LOCUS       DATAIW010000248         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_185140, whole genome shotgun sequence.
ACCESSION   DATAIW010000248 DATAIW010000000
VERSION     DATAIW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185140"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VHM48_07710"
     CDS             complement(<1..720)
                     /locus_tag="VHM48_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755332.1"
                     /translation="MSTDRSPHEPGTRPSGSAISWRLISAVAAVLLAFVACAPARPTP
                     RDSANGSDVAHASEPAPTGRGRPVESDELGWVKLDGVDLRGAFVASGAASSSTTVLLG
                     ADSATGALASWTTHDGAAWERHWLDGSTFGGGIPQVVVAGGPGFIALGWDIEPDPADR
                     STIWTSVDGIDWQQDPDASGRFEGGYVGYATVGSTVIVATCCAAGAPSFHVSDDGLAW
                     AVAAVPAGPLDGPMHLAANSLE"
     gene            916..2775
                     /locus_tag="VHM48_07715"
     CDS             916..2775
                     /locus_tag="VHM48_07715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2755333.1"
                     /translation="MPTIDADPSLTVPEPLPTPEDGAAFRGLGLDERLVTALAALGYE
                     EPTDIQREAIPSLIAGRDILAEAPTGTGKTAAFALPMVQRLGANRSPGGRPRALVLTP
                     TRELAIQVAEAIHRYGQPFGMRVLPVYGGQAIGQQLRGLARGVDVLVATPGRAVDHLT
                     RASIALDAIEVVVLDEADEMLDMGFAEDLEAILSATPPTRQTALFSATISAPIAKIAK
                     RHLREPVRVRIDRERRSRETAAKVRQVAYVVRRPDKSVALGRILDLEDPTSALVFCRT
                     RGEVDELAQRLSAQGHDTAALHGGMVQEQRDRVMGRFREGSIDVLVATDVAARGLDIE
                     HVSHVVNYDIPANPDAYIHRIGRTGRAGREGVAITLVEPREHRLLRNIETAIRTRLEV
                     AQIPTVADLRERRLEVLRSSLREAILGDGLDRFRAVVEPLAEEFDVLDVALAAVSLAE
                     AAAGRDGDNAATEIAPASLFGESRSPHGGGPVRPDRSGPGSGRPGSGPRRGPGGPRDG
                     GGPSGPGGSGRAPGRDDGAWTRLWIGAGRETGVRPGDLVGSITNEAGVPARAIGSIQI
                     SGRFSIVEVAEGAADDIVRAMRGVLLRGKPVQIRRDREAAAPFVQPRPPRTGI"
     gene            2944..>3127
                     /locus_tag="VHM48_07720"
     CDS             2944..>3127
                     /locus_tag="VHM48_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755334.1"
                     /translation="MILIAVVALVAACGATVQPSPPVASSSTAPSLATGSVDPAESAP
                     DPGDGGQFELPTPGCPS"
BASE COUNT          412 a         1188 c         1125 g          402 t
ORIGIN      
        1 ctccaggctg ttggccgcca ggtgcattgg cccgtcgagc ggcccggcgg gcacggcggc
       61 aacggcccag gcgaggccgt cgtcggaaac gtggaaggac ggcgcgccgg ccgcgcagca
      121 ggtcgccacg atgaccgtcg agccgacggt cgcgtacccg acgtagccgc cctcgaatcg
      181 cccgctcgcg tcggggtcct gctgccaatc gattccgtcg accgaggtcc agatcgtcga
      241 ccgatccgcc gggtccggct cgatgtccca gccgagggcg atgaacccgg gcccacccgc
      301 gaccacgacc tgcgggatgc ccccgccgaa cgtcgaacca tcgagccagt gccgctccca
      361 ggcagcgccg tcgtgggtcg tccacgacgc gagggcgccc gttgcgctgt cggcgcccaa
      421 caggactgtg gtcgacgagg acgccgcccc gctcgccacg aatgccccgc gcaggtcgac
      481 gccatcgagc ttcacccagc cgagctcgtc gctctcgacc ggccgtccgc ggccggtcgg
      541 cgcgggctcc gacgcgtggg cgacgtccga cccgttcgct gagtcccgcg gtgtcggcct
      601 cgccggcgcg caggcgacga aggcgagcag gacggcggcc acggcgctga tgagccgcca
      661 gctgatcgca ctcccggacg ggcgcgtccc cggctcatgc ggcgaccggt cggtcgacat
      721 gggatggctc cctcggatga cgtagtcgga tcggccacgc gtgatctggg gacgctcgac
      781 cggagcgtac attgcgttgg cggtttcagt gtcggcgggc cgacaggcgg gctgcgcgcc
      841 gggccaacgc cggacgccgg gcggccggtc cgcgtgaccg cggccccgat ccgcgatcca
      901 gccgatactg ggcaggtgcc caccatcgac gccgacccga gcctgaccgt ccccgagcct
      961 cttcccactc cggaggacgg cgccgcgttc cgtgggctcg gcctcgacga gcggctcgtg
     1021 accgcgctcg cggcgctcgg ctacgaggag ccgaccgaca tccagcgcga ggcgatcccg
     1081 agcctcatcg ccggccggga catcctcgcc gaggccccga ccggcaccgg caagaccgcg
     1141 gcgttcgccc tgccgatggt ccagcgcctc ggcgccaacc gatcgccggg cggccggccg
     1201 cgcgccctcg tcctgacccc gacccgcgag ctcgcgatcc aggtcgccga ggcgatccac
     1261 cgctacggcc aacccttcgg gatgcgcgtc ctgcccgtgt acggcggcca ggcgatcggc
     1321 cagcagctgc gcggcctcgc ccgcggcgtc gacgtcctcg tggccacgcc gggtcgggcg
     1381 gtcgaccacc tgacccgggc gagcatcgcc ctcgacgcga tcgaggtcgt cgtcctcgac
     1441 gaggccgacg agatgctcga catgggcttc gccgaggacc tcgaggcgat cctgtcggcg
     1501 acgcctccga cccgccagac ggcgctcttc tcggcgacga tctccgcgcc gatcgcgaag
     1561 atcgcgaagc gccacctgcg cgagcccgtc cgcgtccgga tcgaccgcga gcgacgcagt
     1621 cgcgagaccg cggccaaggt ccgccaggtt gcgtacgtcg tccggcggcc cgacaagtcg
     1681 gtggcgctcg ggcgaatcct cgacctcgag gacccgacgt cggccctcgt cttctgccgt
     1741 acccgcggcg aggtcgacga gctcgcccag cggctgtcgg cccagggcca cgacacggcg
     1801 gccctccacg gcgggatggt ccaggagcag cgcgaccggg tcatgggccg cttccgcgag
     1861 ggatccatcg acgtcctcgt tgccaccgat gtcgccgccc gcggcctcga catcgagcac
     1921 gtctcgcacg tggtgaacta cgacatcccg gccaaccccg atgcgtacat ccatcgcatc
     1981 gggcggacgg gtcgggccgg ccgcgaaggc gtcgcgatca ccctcgtcga gccgcgcgag
     2041 catcgcctgc tgcggaacat cgagacagcg atccggacgc gtctcgaggt cgcccagatc
     2101 ccgaccgtcg cggacctgcg ggagcgccgc ctggaggtgc tccggagcag cctccgcgag
     2161 gcgatcctcg gcgacggcct ggaccggttc cgggcggtcg tcgagccgct cgcggaggag
     2221 tttgacgtcc tcgacgtggc gctggccgcg gtgagcctcg ccgaagccgc ggcgggccgc
     2281 gacggagaca acgcggcgac ggagattgcc ccggcttccc tgttcggcga gtcgcgatcg
     2341 ccgcacggcg gtgggccggt acgcccggac cgtagtggac cgggcagcgg cagaccgggc
     2401 agcggcccgc ggcgcggacc gggcggtcct cgggatggcg gcggtccgtc cgggcccggt
     2461 ggctccggtc gggcgccggg tcgcgacgac ggcgcgtgga cccgtctctg gatcggtgcc
     2521 ggccgggaga ccggcgtccg gccgggcgat ctcgtcggct cgatcacgaa cgaggccggc
     2581 gtccccgccc gggcgatcgg ttcgatccag atctccggtc gcttctcgat cgtcgaagtg
     2641 gccgagggcg cggccgacga catcgtccgg gcgatgcgcg gcgtcctgct ccgcggcaag
     2701 cccgtccaga tccgccgaga ccgcgaggcg gccgcgccgt tcgtccagcc ccgcccgccc
     2761 cgaaccggaa tctgatcgtc gtcgggcctg gctcgaccgc tcgccgagca tccgacggaa
     2821 cccgattcgt cacacccgcg gtccggcggc gtcttggggg ggggtgacga ccgaccgacg
     2881 atcgaagagg agaccgcgat ggggccgatc ggccggcaag ggatcaggtc cggttccgga
     2941 gccttgatcc tgatcgccgt cgtggcgctg gtcgcggcct gcggagcgac cgtccagccg
     3001 tcgccgccgg tcgcgtcgtc gtcgactgct ccgtccctgg cgaccggatc ggtcgatcca
     3061 gccgagtcgg cacccgaccc gggcgacggc ggccagttcg agctcccgac cccaggctgt
     3121 ccatccc
//
LOCUS       DATAIW010000249        10382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_74426, whole genome shotgun sequence.
ACCESSION   DATAIW010000249 DATAIW010000000
VERSION     DATAIW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74426"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM48_07725"
     CDS             <1..235
                     /locus_tag="VHM48_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2755335.1"
                     /translation="STVEVKAGGNFTLHFDNQDSAPHNVAIYTDSSASSAVSVGEIVA
                     SGKKDQVVPAPKAGTYFFRCDVHTNMTGTIVAK"
     gene            611..1120
                     /locus_tag="VHM48_07730"
     CDS             611..1120
                     /locus_tag="VHM48_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755336.1"
                     /translation="MTSPFVTFVRFMPRASQRTRRLLVAGAFLGFPLLNLGYATLVAP
                     GRLSSTVWAPIAIALFSATLIGVVAIYGYARGRADMKAGLDERQRQVRDQAWIHAYEL
                     LILAVTVVVGAMALVTSFGGPLTIGMSELLPLAIGIGLYLPLLPSAMLTWSEPDVPAE
                     LDDEPAAQR"
     gene            1117..1401
                     /locus_tag="VHM48_07735"
     CDS             1117..1401
                     /locus_tag="VHM48_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755337.1"
                     /translation="MTRRPATPIHNRIAVLRAERGLSRQDLADALDINYQTVGYLERG
                     EYNPSLDLAFAIAALFELPIEAVFSATPFEPMSARLYGRTTDPGEPTTVA"
     gene            1394..2284
                     /locus_tag="VHM48_07740"
     CDS             1394..2284
                     /locus_tag="VHM48_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2755338.1"
                     /translation="MLEITDLSKRYGPVVALDGATFSVRPGRLVGFLGPNGAGKTTAM
                     RSIFGLARPDHGTIAWQGRPIDRAHRLRFGYMPEQRGLYPRMKVADQLSYFAQQHGMP
                     AKAAKAATTRWLDGLGLGDRAGARLEALSHGNQQRVQLGAALVHDPVLLVLDEPFSGL
                     DPLGIATMTGILRERAAAGVGVVFSSHQLDLVEDVCEDVVIINRGRIVAAGAIDDLRA
                     ASARRHLEVEVMGNGNAWLAGRSDLVVVEQRGDFARLLVPADVDLAAVLAAASAAGAV
                     RRFAFQPPTLSEIFMEVVRS"
     gene            2281..3420
                     /locus_tag="VHM48_07745"
     CDS             2281..3420
                     /locus_tag="VHM48_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755339.1"
                     /translation="MSRRRAIWLVARRELIERGRSRAFLLSLLLSVAIIVAGVFLPSL
                     VGGGANAQHLGIVGTPPAGLVDGIERSAKNVGIGLTVDGVADLQAGEGRLRDGSLDAV
                     LVVPPDGSTPTYVVKSRGNQLLQQVVSASFTLQPQIAFRELEPGDPNRDISFVFANIG
                     VILLFISIFTFGSWVLTGVVEEKQSRVVEVVLSTIEPRDLLIGKVLGIGVLGLVQLVV
                     MVTVGLGAGVAGGRFSLPSTTGSAVAMLFVWFILGYAFYSTALGVLGALASRMEEASN
                     ASSPVSFIAMGGYFFSLIVALNDPGGTAARIATFIPPVSPMVVPLRAALGAIEPWEII
                     ASAALMVAAIWVLFVVGGRVYSGAVLQTGARMKLRDAWRASGRGG"
     gene            complement(3424..4422)
                     /locus_tag="VHM48_07750"
     CDS             complement(3424..4422)
                     /locus_tag="VHM48_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2755340.1"
                     /translation="MVIPPAIAALPRFGLIDRPSPLTPLPRFSAALGGGAEIWIKRED
                     LLPLAFGGNKLRNLEFLVGAALADGADTLITSGRRWSNHCRLTAAAGARAGLPVHLVL
                     TGPPPLRGDEGPNQRLDEMLGATVHVSATDDRAERAAVVERIAADVRAGGGHPFVIGV
                     GGTGPIGAAGQVLAGLEVADQLGAAGIGAATVVLPSATGGTQAGVLAGLALAAPPLRD
                     VLGIAVAAPAAELRPTILGILEGLAPLVGGVLHGDAVVLEDDQLGDGYGRPTAAADEA
                     TRLLARTEGILVDPIYTAKALAGLVARVRDGRLSGPVVFWHAGGTPGIFERLPSGG"
     gene            4476..5723
                     /locus_tag="VHM48_07755"
     CDS             4476..5723
                     /locus_tag="VHM48_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2755341.1"
                     /translation="MPVDRPRRVLYRDAALADGRSDRLQLGISVLVDGDRVAWIRPTD
                     AEEDPTAASSLPAPEVVDAGGTTIVPGMVDGHSHLTMPGGSHWIDRGADPPEEAVATA
                     ARNAALMTQSGVRWARDVGSAVARDPVDGRVRALTLGVRDRSRGRAGWPNIRAAGTWI
                     GGPGALGDLGLEAADGDVLVAAAEGQLDDGADFIKLYLDGADPTVSPWSIAEIERVVA
                     AVHARGARVTAHSGRLDGATKGVAGGVDALEHGFELDAAVAAEMARRGTFLVSTLAVF
                     RSWESFGATTTIERFTGPEGRERIAARRERAEESVRIAHRAGVRIATGTDFGGGSTRA
                     NQLAWEVEALVAAGLQPWEALGAATWRGGELLGEPDAGVLRDGGPADFSLVHGDPLSD
                     PTALWRVWRVSWSADPVVVRGEA"
     gene            5731..6114
                     /locus_tag="VHM48_07760"
     CDS             5731..6114
                     /locus_tag="VHM48_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2755342.1"
                     /translation="MATRIRPDERSTSTGQNADMERSIAISAERVGSVGLYSSVVTTA
                     PSARTRIHHHGECETSIWIASGRARYTFGPTGLEESMEAEAGDFVYIAAGEVHVEENA
                     SDTEPLVVVISRNCPGSVVHYLDEG"
     gene            6111..7127
                     /locus_tag="VHM48_07765"
     CDS             6111..7127
                     /locus_tag="VHM48_07765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2755343.1"
                     /translation="MSLVDLEPLDGLLHRHGLGGATEEAFPNDGWSGAAMTRLRNGDG
                     RAYILKRDSLERDWIARATADRPVPREAWFAAHGPALPWPVRAPYLGAGWDDSAHEAG
                     ILMPDLSSVLFDWNSTLTVEQLDRVIDALAVLHSHPWGPALDASAAHWTPWQERLTLI
                     CRPSLERAGPAREAVADRLLPGWDAWDRLATPTARAVIADLAADPGPLLDALAREPAT
                     LLHGDLKLANVGIAADGAVELVDWQMVMVAPVAVELGWFLVSNVNALPEPAGDVLARY
                     WTRRSMDGARQDDLTILVGLLIRGWRKGADAVAGVTLASGVSAADDLAWWCERAVEAA
                     DRIL"
     gene            complement(7131..7781)
                     /locus_tag="VHM48_07770"
     CDS             complement(7131..7781)
                     /locus_tag="VHM48_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755344.1"
                     /translation="MSTRERPADRGRRRASEAFQLLGRDARSARIGAGLSLRVVAAAT
                     GTSHQQLLRFERGRIDQVSVQDVGAWCATVGLDLVVRAFPAGDPIRDAGQQRLLDRLR
                     PHIHASLTWRTEVPLPIRGDLRAWDLLIEGRGWRIAVEAETVLDDLQALERRLHRKQR
                     DGGIDVLILLVADTRRNRRAVEAAPASFAGFSHDARATLHALRGGTHPGHSAIVFL"
     gene            complement(8203..9006)
                     /locus_tag="VHM48_07775"
     CDS             complement(8203..9006)
                     /locus_tag="VHM48_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2755345.1"
                     /translation="MNAALFGHLWRSQRTKLLIVCVAMAAWSVFLPVIYQSFGSQMRA
                     LVDSGIIPRELMNFGGGDVFSLGGAIALGYVHPISIILIAVFAIGFTTAAIAGERQRG
                     TLEVLLARPLSRRTVYTTELAAVLVFIGLVLAAASVGTVIGATLAGVLDELAVERLPM
                     LWLNGMLLWGAFAAIALAASVSFDRFTPAIGVTLAIVVVMYFLDILGSLWPDAKGLQA
                     YSLFHYLSARDILSGTVSLSGFTLLAAVGAAAVAYALAVFPRRDLAAPS"
     gene            complement(9003..9917)
                     /locus_tag="VHM48_07780"
     CDS             complement(9003..9917)
                     /locus_tag="VHM48_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755346.1"
                     /translation="MIRALEDLTISVAEGEIFGFLGPNGAGKSTAIRLLLGFIHPTRG
                     GGSVLGLNIVRDSVAIRSRVGYLPGGIALYDSLNGERLLDYLGELTGRPSTRRAELLD
                     RLEMSARTLTRPVRDYSRGMRQKIGIIQALQHDPELAILDEPTEGLDPLMQRAFYAIL
                     DDLKAAGRTIFFSSHILSEVERVCDRVAIVRHGRLVALQDVASLLEHRKRNVELRAID
                     GDLPALDGIAGVSGLGRTADGRLTCQLEGDVGPFLGAIAGHRITDLTIEAAHLEEAFL
                     ELYEDAESEDDDGPATALPVGPPVGTAR"
     gene            10101..>10382
                     /locus_tag="VHM48_07785"
     CDS             10101..>10382
                     /locus_tag="VHM48_07785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2755347.1"
                     /translation="MPKTVPTGPGLELGSLPFSKVVEANGFVFLAGQIGDAPGSNGPV
                     PGGIEAETRATLENVGRLLKAVGLDYDDVVKCTVYIVDFDEFAAMNTVYR"
BASE COUNT         1477 a         3707 c         3703 g         1495 t
ORIGIN      
        1 gtcgacggtc gaggtgaagg ccggcggcaa cttcaccctc cacttcgaca accaggactc
       61 ggcgccgcac aacgtcgcga tctacaccga cagctccgcc tcgagcgcgg tctcggtcgg
      121 cgagatcgtc gcgtcgggca agaaggacca ggtcgtcccc gcccccaagg ccggcaccta
      181 cttcttccgc tgcgatgtcc acacgaacat gacggggacg atcgtcgcga agtaggcgat
      241 cgagcggctc cgcagccacc gaacgaaccc cgggctccgg cccggggttc ttcgattccc
      301 ggtgcggttc cgccgcgatc cggccacatt gccacccacg gaccgttggg ctcgatcctg
      361 cagcccggcg agggttcacc tgcggtcgcc gattggtccg gtcgcctgga ccgaggcgtg
      421 attcgggaac aacaagtggc cgggaccggt cgcgcaggcg aagcgagccg tccggatctg
      481 tcccagtggt ctgacggtgg gaatctggcc aggtccaagg tcccgtctcg tgaaactatt
      541 ggccatattg acagactgtt gtattgatcg tacatttcgt ggcatagacc gccaccttga
      601 ggtccacgcc atgacatcgc ccttcgtcac attcgtccgg ttcatgcccc gggcatcgca
      661 acgtacccgc cgccttctcg tggcgggtgc gttcctgggg tttccccttc tcaacctcgg
      721 atacgcgacg ctcgtcgcgc cggggcgcct gtcctcgacg gtctgggcgc cgatcgcgat
      781 cgccctgttc tcggcgacgc tcatcggggt cgttgccatc tacggctacg ctcgaggcag
      841 ggctgacatg aaggccggcc tcgacgagcg ccagcgccag gtccgggacc aggcctggat
      901 ccatgcctac gagctcctga tcctcgcggt caccgtcgtg gtcggcgcaa tggccctcgt
      961 gacctcgttc ggcggcccgc tgaccatcgg catgagcgag ctgctgccac tcgccatcgg
     1021 gatcgggctg tacctcccgc tgcttccgtc agccatgctg acctggagcg aacccgacgt
     1081 gcccgcggaa ctcgacgacg aaccggccgc ccagcgatga cccgccgccc ggcgaccccg
     1141 atccacaacc ggatcgccgt cctccgcgcc gagcgaggac tgtcgcgcca ggacctggcc
     1201 gacgccctcg acatcaacta ccagacagtc ggctatctcg agcgcggcga gtacaacccg
     1261 agcctcgatc tcgcattcgc gatcgccgcg ctcttcgagc tgccgatcga ggccgtcttc
     1321 tcggccacgc cgttcgagcc gatgtcggcc agactgtacg gacgaacgac cgatcccggg
     1381 gagccaacga ccgttgcttg agatcaccga cctgtcgaaa cgctatggac ccgtcgtcgc
     1441 cctcgacggc gcgacgttct cggtgcggcc ggggcggctg gtcggcttcc tcggcccgaa
     1501 cggggccggc aagacgaccg cgatgcgcag catcttcgga ctcgcccggc ccgaccacgg
     1561 gacgatcgcc tggcagggcc gcccgatcga ccgggcgcat cggctccgct tcggctacat
     1621 gcccgagcag cgcgggctgt atccgcggat gaaggtcgcc gaccagctga gctacttcgc
     1681 ccagcagcac ggcatgccgg cgaaggcggc aaaggcggcg acgacccgct ggctcgacgg
     1741 gctcgggctc ggcgatcggg ccggcgccag gctcgaggcg ctctcccacg gcaaccagca
     1801 gcgggtccag ctcggggcgg ccctcgtcca cgaccccgtg ctgctcgttc tcgacgagcc
     1861 gttcagcggc ctcgacccgc tcgggatcgc gacgatgacg ggcattctgc gcgagcgcgc
     1921 cgcggccggc gtcggcgtcg tcttctccag ccatcagctc gacctcgtcg aggatgtctg
     1981 cgaggacgtc gtgatcatca accgcggccg gatcgtcgcc gcgggggcga tcgacgacct
     2041 gcgcgccgcg tccgcccggc gtcacctgga ggtcgaggtc atgggcaacg gcaacgcgtg
     2101 gctcgccggc cgatcggatc tggtcgtggt cgagcaacgc ggcgacttcg cccgcctcct
     2161 cgtcccggcc gatgtcgacc tggccgccgt cctggccgcg gcctcggcgg ccggagcggt
     2221 tcgccggttc gccttccagc cgccgaccct gtccgagatc ttcatggagg tcgtccggtc
     2281 atgagccgcc gccgcgcgat ctggctcgtg gcccggcgcg agctcatcga gcgcggccgg
     2341 agccgggcgt tcctgctctc gctcctgctg tcggtcgcga tcatcgtcgc cggcgtcttc
     2401 ctgccgtccc tcgtcggcgg cggcgcgaac gcccagcacc tcggcatcgt cgggacgccg
     2461 ccggcggggc tcgtcgacgg gatcgagagg tcggcgaaga atgtcggaat cggcctgacg
     2521 gtcgacgggg tggcggacct gcaagccggc gagggccgcc tgcgcgacgg ctcgctcgat
     2581 gccgtcctgg tcgtgccgcc cgacggctcg acgccgacct acgtcgtcaa gagtcggggc
     2641 aaccagctcc tccagcaggt cgtctccgcg agtttcacgc tccaaccgca gatcgcgttc
     2701 cgcgagctcg aacccggcga tccgaaccgt gacatctcgt tcgtgttcgc caacatcggg
     2761 gtgatcctgc tgttcatctc gatcttcacg ttcgggtcgt gggtcctgac cggagtcgtg
     2821 gaggagaagc agagccgggt cgttgaggtc gtgctctcga cgatcgagcc gcgcgacctg
     2881 ctgatcggca aggtcctcgg gatcggggtg ctcgggctcg tccagctcgt cgtgatggtc
     2941 acggtcgggc tcggcgcggg ggtggccggc ggtcgcttca gcctgccgtc gacgaccgga
     3001 tcggcggtcg cgatgctgtt cgtctggttc atcctcggct acgcgttcta ctcgaccgcg
     3061 ctcggcgtgc tcggcgcgct tgcgtcgcgg atggaggagg cctcgaacgc ctcgagcccg
     3121 gtcagcttca tcgcgatggg cggctacttc ttctcgctga tcgtcgcgct caacgatccg
     3181 ggcgggaccg ccgcgcgaat cgcgaccttc atcccgccgg tctctccgat ggtcgtgccg
     3241 ctgcgggccg ccctcggggc gatcgagcca tgggagatca tcgcgtcggc ggcgctcatg
     3301 gtcgcggcga tctgggtcct gttcgtcgtc ggcggacggg tctactccgg tgcggtgctc
     3361 cagacgggcg cccggatgaa gctgcgcgac gcgtggcggg cgtccggccg cggcggctga
     3421 ggctcaccct ccggacggca accgctcgaa gatcccgggc gtgccgccgg cgtgccagaa
     3481 cacgaccggc ccgctgagcc ggccgtcgcg gacgcgggcg acgagcccgg ccagcgcctt
     3541 ggccgtgtag atcgggtcga cgaggatgcc ctccgtccgg gcgagaagcc gcgtggcctc
     3601 gtccgccgcc gccgtcggac ggccgtatcc gtcgccgagc tggtcgtcct cgagaacgac
     3661 cgcatcaccg tggagcaccc cgccgacgag cggcgcaagc ccctcgagga tcccgaggat
     3721 cgtcggtcgc agctccgccg cgggcgcggc gaccgcgatg ccgagaacgt cgcgcagcgg
     3781 cggcgcggcc agggccagac cggcgaggac gcccgcctgg gtaccgcccg tcgccgacgg
     3841 caggacgacg gtggcggcgc cgatgccggc ggcgccgagc tggtccgcga cctcgagtcc
     3901 ggcgaggact tgccccgccg cgccgatcgg gcccgtccct ccgacgccga tcacgaacgg
     3961 gtgaccgccg ccggcgcgga cgtcggcggc gatcctctcg acgaccgccg cccgttccgc
     4021 ccggtcgtcg gtcgcgctga cgtggacggt cgccccgagc atctcgtcga gccgctggtt
     4081 cgggccctcg tcgccgcgga gcggcggcgg gccggtcagg acgagatgga ccggcagccc
     4141 ggcgcgtgcc ccggcggcgg cggtcaggcg gcaatggttc gaccagcgcc ggcccgaggt
     4201 gatcagcgtg tcggctccgt cggccagcgc ggccccgacc aggaactcga ggttccgcag
     4261 cttgttgcca ccgaacgcga ggggcaggag gtcctcgcgc ttgatccaga tctcggcgcc
     4321 gccgccgagg gcggccgaga accgcggcag cggcgtcagc ggcgacggtc gatcgatcag
     4381 gccgaaccgc ggcagggccg cgatcgcggg cggaatcacc atcgaccgat cgtagccggt
     4441 gcgcggtccg cccacggtgc tagtctcgcg acgtcatgcc ggtcgaccgc cctcgccgcg
     4501 ttctctatcg cgatgccgcc ctcgccgacg ggcggagcga ccgcctccag ctcgggatca
     4561 gcgtcctcgt cgacggcgac cgggtcgcct ggatccggcc gaccgacgcg gaggaggacc
     4621 cgacggccgc gtcatcgctg ccggcaccgg aggtcgtcga cgccggcggg accacgatcg
     4681 tcccgggcat ggtcgacggc cacagccacc tgacgatgcc gggcggctcg cactggatcg
     4741 atcgcggcgc ggatccgccc gaagaggccg tcgccaccgc cgcgcgcaat gccgcgctca
     4801 tgacccagtc gggcgtccgc tgggcgcgcg atgtcggatc ggccgtcgcc cgcgacccag
     4861 tcgacggccg cgtgcgggcg ctgactctcg gcgtccgcga ccggtcgcgc ggtcgtgccg
     4921 gctggccgaa catccgcgcg gcgggcacgt ggatcggcgg gcccggtgcg ctgggcgacc
     4981 tcggcctgga ggccgccgat ggcgacgtgc tggtcgcggc cgcggagggc cagctcgacg
     5041 atggcgccga cttcatcaag ctctacctcg acggcgccga cccgacggtc agcccgtggt
     5101 cgatcgccga gatcgaacgg gtcgtcgctg cggtccacgc tcgcggcgcc cgggtcaccg
     5161 cccacagcgg ccgcctcgac ggggcgacga agggcgtcgc cggcggcgtc gacgccctcg
     5221 agcacgggtt cgagctcgac gctgcggtcg ccgccgagat ggcccggcgc ggcacgttcc
     5281 ttgtctcgac gctcgcggtc ttccgctcct gggagagctt cggggcgacg acgacgatcg
     5341 agcggttcac cggtcccgaa gggcgggagc ggatcgccgc ccgccgcgag cgggccgagg
     5401 agtcggtccg gatcgcccac cgggccggcg tccggatcgc gaccggcacg gacttcggcg
     5461 gaggctcgac gcgggcgaac cagctcgcct gggaggtcga ggcgctcgtc gccgcgggcc
     5521 tccagccgtg ggaggcgctc ggcgcggcga cgtggcgcgg cggggagctc ctcggcgagc
     5581 ccgacgccgg cgtcctccgc gacggcgggc cggccgactt ctcgctcgtc cacggcgacc
     5641 cgctgtcgga cccgacggcc ctctggcggg tctggcgcgt ctcgtggtcg gcagacccgg
     5701 tcgtcgtccg gggcgaggcc tgaccgaccc atggccaccc ggatccggcc cgacgagcgc
     5761 tcgacctcga ccggccagaa cgccgacatg gagcgctcga tcgcgatctc ggccgaacgc
     5821 gtcggctcgg tcgggctgta ttcgtccgtc gtgacgacgg cgccgagcgc ccggacccgg
     5881 atccaccacc acggcgagtg cgagacgtcg atctggatcg cctccgggcg ggcccgctac
     5941 acgttcggcc cgaccgggct cgaggagtcg atggaggcag aggccggcga cttcgtgtac
     6001 atcgccgccg gcgaggtcca cgtcgaggag aacgcgtccg acacggagcc actcgtggtc
     6061 gtgatcagcc ggaattgtcc gggctcggtc gtccactacc tcgacgaggg atgagcctgg
     6121 tcgacctcga accgctcgac ggcctcctgc accggcacgg cctcggcggc gcgaccgagg
     6181 aggccttccc gaacgacggg tggtcgggag cggcgatgac gcggctccga aacggtgacg
     6241 ggcgggcgta catcctcaag cgtgactcgc tggagcgcga ctggatcgcc cgggcgacgg
     6301 ccgatcggcc cgtcccgcgc gaggcctggt tcgccgccca tggcccagcg ctgccctggc
     6361 cggtccgggc gccatatctc ggggccggct gggacgacag cgcacacgag gccgggatcc
     6421 tgatgccgga cctgagcagc gttctcttcg actggaactc cacgctgacg gtcgagcagc
     6481 tcgatcgcgt gatcgacgcg ctcgcggttc tccactccca cccgtgggga ccggctctcg
     6541 acgcgagcgc ggcccactgg acgccgtggc aggaacgcct gaccctgatc tgccggccgt
     6601 ccctggagcg cgcgggtccg gctcgcgagg cggtcgccga tcggcttctg cccggctggg
     6661 atgcctggga ccgactggcg acgcccacgg cccgggcggt catcgccgac ctggcggctg
     6721 acccggggcc gctgctggac gccctcgccc gggagcccgc gacgctcctc cacggcgacc
     6781 tcaagctggc caacgtgggc atcgccgcgg acggcgccgt cgagctcgtc gactggcaga
     6841 tggtcatggt tgcaccggtc gccgtcgagc tcggctggtt cctggtatcc aacgtgaacg
     6901 ccctgcccga gcccgccggc gacgtgctcg ctcgctactg gacgcgtcga tccatggatg
     6961 gcgcccgtca ggacgacctg acgatcctcg tcggcctcct catccggggc tggcggaagg
     7021 gcgcagacgc agttgccggg gtcacgctcg cgtccggcgt cagcgccgcc gacgacctcg
     7081 cctggtggtg cgaacgcgcg gtcgaggcgg ccgatcggat cctctgacga ctagaggaac
     7141 acgatcgcgc tgtgaccggg atgcgtgccg ccgcggaggg cgtgaagcgt cgcccgggcg
     7201 tcgtggctga aaccggcaaa cgaagccggc gcggcctcga cggcacggcg gttccgacgg
     7261 gtgtcggcga cgagcaggat gagcacgtcg attccgccgt cccgttgctt gcggtgcagg
     7321 cgtcgctcga gggcctggag atcgtcgaga acggtctccg cttcgacggc gatccgccac
     7381 cctcgaccct cgatgagcag gtcccatgct cgcaggtcgc cgcggatcgg cagcgggacc
     7441 tcggtccgcc acgtgaggct cgcgtggatg tggggccgaa ggcgatccag gagccgctgc
     7501 tggccggcgt cgcggatcgg atcgcctgcc ggaaaggcgc gcacgacgag atcgagcccg
     7561 accgtcgcgc accaggcgcc gacatcctga acggagacct gatcgatcct gccgcgctcg
     7621 aagcggagga gctgctgatg ggatgtgccc gtcgccgcgg cgacgacgcg caggctcaat
     7681 cctgcgccga ttcgcgcgga acgagcatcg cggccgagga gctggaatgc ttcgctggct
     7741 cggcgccggc cgcgatctgc cggccgctcc ctcgtgctca ccccgtgatg atgacggccg
     7801 ccgatttgtt gccggttatt tggtcgctct cggccgagcc ggtcggccga tcctggtccg
     7861 cctcacggac caagcgacaa ccgggctgcc ggcttgggct ggttcgagcc cgccagtcgt
     7921 cgtgtggtcc acgtcacgga ccacgggacg accgaaggcc cgagcgtgcc ctcgatgcgt
     7981 gacgtattgg cgccctggcc gtcgcttggt ccgtcgcgcg gaccacgggc cggtgaccag
     8041 gccccactcc cgcggcgccg gtcggccgat cctggtccgc gacgcgcgga tcggcattga
     8101 gcgccgcgaa cggcccccgg tccgggtcgc gccactcgcg gaggacgagc cgctcggtgg
     8161 tccggcgcgg gatcggcggg ccctccatcg gggcggcgac gctcagctcg gggcggcgag
     8221 atcgcgccgg gggaagacgg caagtgcata ggcgacggcg gcggccccca cggccgccag
     8281 gagcgtgaac ccggacaggc tgaccgttcc ggagaggatg tcgcgggcgg acaggtaatg
     8341 gaacagcgag tacgcctgga gacccttcgc atccggccag agcgatccca ggatgtcgag
     8401 gaagtacatg acgacgacga tggccagggt caccccgatc gccggcgtga accggtcgaa
     8461 cgagaccgag gccgcgaggg cgatcgcggc gaaggcgccc cacagcaaca tgccgttgag
     8521 ccagagcatc ggcagtcgct cgaccgccag ctcgtcgagg accccggcga gggtcgcccc
     8581 gatcaccgtt ccgaccgatg cggcggccag gaccaggccg atgaagacca ggaccgcggc
     8641 aagctcggtc gtgtagacgg tgcggcggga cagcggccgg gcaaggagga cctcgagcgt
     8701 gcctcgttgg cgttcgcccg cgatcgcggc ggtcgtgaag ccgatcgcaa agacggcgat
     8761 caggatgatc gagatcgggt ggacatagcc gagggcgatc gccccgccga ggctgaagac
     8821 gtcgccgccg ccgaagttca tcagctcgcg cgggatgatc ccggagtcca cgagggcccg
     8881 catctggctc ccgaacgact ggtagatcac gggcaggaac acgctccagg ccgccatcgc
     8941 gacgcagacg atcaggagct tggtccgctg cgaccgccag agatgaccga acagagcggc
     9001 gttcatcgcg cggtgccaac tggcggtccg acgggcaagg ccgtcgccgg gccgtcgtca
     9061 tcctcggact cggcgtcctc atagagctcg aggaacgcct cctcgagatg ggccgcctcg
     9121 atcgtgaggt ccgtgattcg atggccggcg atcgcgccaa ggaacggccc gacgtcgccc
     9181 tcgagctggc aggtcagccg cccatcggcc gtccggccga gcccgctgac accggcaatc
     9241 ccgtcgagcg ccggcaggtc gccgtcgatc gcccgcagct cgacgttccg cttgcggtgc
     9301 tcgagcaggc tggcgacgtc ctggagggcg accagccggc cgtgacgaac gatcgcgacc
     9361 cggtcgcaca cccgttcgac ctcggacagg atgtgcgacg agaagaagat cgtccgaccg
     9421 gccgccttga ggtcgtcgag gatcgcgtag aacgcccgct gcatgagcgg gtcgaggccc
     9481 tcggtcggct cgtcgaggat cgccagctcc gggtcgtgct ggagcgcctg gatgatcccg
     9541 atcttctggc gcatcccgcg cgaatagtcg cggaccgggc gggtgagcgt gcgggcggac
     9601 atctccaggc gatccagcaa ctctgcccga cgcgtcgatg gccggccggt cagctcgccg
     9661 aggtagtcga gcagccgctc gccgttgagc gagtcgtaca gtgcgatccc gcccggcagg
     9721 taaccgaccc ggctccggat cgcgacgctg tcacggacga tattgagccc gagaacgctg
     9781 ccgccgcccc gcgtcgggtg gatgaagccg agaaggagcc ggatcgccgt cgacttgccg
     9841 gcgccgttcg gcccgaggaa gccgaagatc tcgccctcgg cgacgctgat cgtcaggtcc
     9901 tcgagggccc ggatcacacc gaccggatga gaaccgggac ggtgaacccg cgactcccgg
     9961 tggccatagt gcttcgaaag gccgttcgtg ctgagggcat ccatacgagg gcaaggctag
    10021 cgtgtcgcga cgtcgctcga ctggcgggac ccgtagactc gaagcctccg accgcacgcc
    10081 accacgagga gccgtcaccg atgccgaaga ccgtcccgac cggacccggg ctcgagctgg
    10141 gcagcctgcc gttctcgaag gtcgtcgagg ccaacgggtt cgtcttcctc gccggccaga
    10201 tcggcgacgc gccaggctcg aacgggccgg taccgggcgg catcgaggcc gagaccaggg
    10261 caaccctcga gaatgtcggc cggctgctca aggcggtcgg actcgattac gacgatgtcg
    10321 tcaagtgcac ggtctacatc gtcgacttcg acgagttcgc ggcgatgaac accgtctacc
    10381 gc
//
LOCUS       DATAIW010000250         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_104030, whole genome shotgun sequence.
ACCESSION   DATAIW010000250 DATAIW010000000
VERSION     DATAIW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104030"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHM48_07790"
     CDS             <1..627
                     /locus_tag="VHM48_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755348.1"
                     /translation="YGLLGPNGSGKTTLIRLLTGLGRADAGTAEVLRARMPSRVTLGR
                     IGYMTQSDGVYPALTVAENVRFFGTVYGRPDAAAALAALEIVALADRANTITANLSGG
                     QRRRLSLACALVHEPAVLFLDEPTVGVDPLLRVEFWAHFRRLADTGTTIVVSSHVMDE
                     ADRCDDLLFMRAGRVIARGSGAELRSEAGTADLEAAFLRFAGDGSDQR"
     gene            624..1754
                     /locus_tag="VHM48_07795"
     CDS             624..1754
                     /locus_tag="VHM48_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755349.1"
                     /translation="MSARRIAALFRRVAAEIRRDRPSLGILFMAPILLTGLVSFILRE
                     SQPPAITADIVNVAGGPTGVAAALHTGLVAGGATVIDVADEATARSDVEHGRASLAIV
                     LPADPAAGLTVVTLGLDAPGEASQLGVVQKAVQGAVASVTGATARPVSHATIFGQPTD
                     DPVASFAPAIVGFFAYFFVYVLTGVSFLRERTGGTLERLLATPVTRAEIVTGYTLGFG
                     LFATFQVIVLLLWALGTVNVPAIGPLPAFAIGLGIPAAGSPILAFLVVLLLAVGAVSL
                     GIFLSTFARTELQVIQFIPLVLAPQFLLSGVIFPVSSLPAVLQPLVGFMPLNYAVDGL
                     RQVFIRGADLGVGALQLDLAILAAIVVFFGVVASLTIRRDIV"
     gene            complement(1789..2262)
                     /locus_tag="VHM48_07800"
     CDS             complement(1789..2262)
                     /locus_tag="VHM48_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755350.1"
                     /translation="MDATGDPWAWVMAIFGSIAVWFLGLVLISILVAGIGVVVFGPRE
                     IDSLIIPAMATFGVLSVLITAPATGLHPVTLVWVSAAVGIGSAGIQRVLIDRWGELTT
                     CRPGLRLASGVAAGAAVGGVLGFVFYSRAAATVVVGIALVGAVLGGMLSLRGDDR"
     gene            complement(2262..>2540)
                     /locus_tag="VHM48_07805"
     CDS             complement(2262..>2540)
                     /locus_tag="VHM48_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755351.1"
                     /translation="PIERLVRVRIGPVRIDGLKSGRVRALKAPEVRGLGGGGGRSRGD
                     NRAEEEPDIVTTPTAPVARPARPARPVRTAAPPASGPRIRSRRDGRAP"
BASE COUNT          328 a          956 c          862 g          394 t
ORIGIN      
        1 tacggcctgc tcggcccgaa cggctcgggc aagacgaccc tgatccggct cctgaccggg
       61 ctcggccggg ccgacgcggg cacggccgag gtgctgcgtg cccggatgcc gtcgcgagtg
      121 acgctcggcc ggatcggcta catgacccag agcgacggcg tctacccggc gctgacggtc
      181 gccgagaacg tccgcttctt cgggactgtc tatggccggc ccgatgcggc cgccgcgctc
      241 gccgccctcg agatcgttgc cctcgccgac cgggcgaaca cgatcacggc gaacctgtcg
      301 ggcggccagc ggcggcggct gtccctcgcc tgcgcgctcg tccacgagcc ggcggtcctg
      361 ttcctcgacg agccgaccgt cggtgtcgat cccctcctca gggtcgagtt ctgggcccat
      421 ttccggcggc tggccgacac cgggacgacg atcgtcgtgt cgagccacgt catggacgag
      481 gccgaccgtt gcgacgacct gctgttcatg cgggccggcc gcgtgatcgc ccgcggcagc
      541 ggggccgagc tgcggtccga ggccgggacc gccgatctcg aggccgcgtt cctccgattc
      601 gccggcgatg ggtcggacca acgatgagtg cccgtcggat cgctgcgctc ttccgccgcg
      661 tggctgccga gatccggcgc gaccgcccgt cgctcgggat cctcttcatg gccccgatcc
      721 tgctcaccgg ccttgtctcg ttcatccttc gcgagagcca gccgccggcg atcaccgccg
      781 atatcgtcaa tgtcgcgggc ggcccgaccg gggtggccgc ggcgctccat accggcctcg
      841 tcgcgggcgg cgcgaccgtg atcgacgtcg ccgacgaggc caccgcccgc tccgacgtcg
      901 agcatggtcg tgcctcgctc gcgatcgtcc tcccggccga tcccgcggcc ggtctcaccg
      961 tcgtcacgct gggtctcgat gcacctggcg aagcctcgca gctcggggtc gtccagaagg
     1021 ccgtccaggg cgccgttgcg tcggtcaccg gggcgaccgc ccgaccggtg agccacgcaa
     1081 cgatctttgg ccaacccacc gacgaccctg tcgccagctt cgcgccggcg atcgtcgggt
     1141 tcttcgcgta cttcttcgtg tacgtcctga cgggcgtcag cttcctccgc gagcggaccg
     1201 gcgggaccct cgagcggctg ctcgcgacgc cggtcacccg ggccgagatc gtgaccggct
     1261 acacgctcgg gttcggactg ttcgcgacgt tccaggtgat cgtcctgctc ctgtgggcgc
     1321 tcgggaccgt caacgtgccc gcgatcgggc cactgccggc gttcgcgatc ggactcggca
     1381 tcccggccgc cggcagcccg atcctcgcgt tcctcgtggt cctgctgctc gcggtcggcg
     1441 ccgtcagcct cgggatcttc ctctcgacct tcgcccggac cgagctgcag gtgatccagt
     1501 tcatcccgct cgtcctggcc ccgcagttcc tcctgtcggg cgtgatcttc ccggtcagct
     1561 cgctgccggc ggtgctccag ccgctggtcg gcttcatgcc gctcaactac gcggtggacg
     1621 ggctccgcca ggtgttcatc cgcggcgcgg acctcggcgt cggcgcgctc cagctcgatc
     1681 tcgcgatcct ggccgcgatc gtggtgttct tcggcgtcgt agcgtccctc acgatccggc
     1741 gcgacatcgt ctgaccgtgg cccaggccct cggtgacaag cttcgcagtc accgatcatc
     1801 acctctcagg ctcagcatgc cgccgaggac cgccccgacc agtgcgatgc cgacgacgac
     1861 ggtcgccgct gcacgagaat agaacacgaa gccgagcacg ccgccaacgg ccgcgccggc
     1921 cgcgacgccg ctggcgaggc ggagccctgg ccggcaggtc gtgagctcgc cccaacgatc
     1981 gatgagcacg cgctggatcc cggcggagcc gatgccgacg gcagccgaca cccagacgag
     2041 ggtgaccgga tgtaaccccg tcgccggcgc ggtgatcaag acgctcagga cgccgaaggt
     2101 cgccatggcc gggatgatca gcgaatcgat ctcccggggg ccgaacacga cgacgccgat
     2161 tcccgccacc aggatcgaga tcaggaccag tccgaggaac cacaccgcga tcgaaccgaa
     2221 gatggccatg acccaggccc acggatcgcc ggtggcgtcc atcagggggc gcggccgtcc
     2281 cgtcgtgatc ggattcgcgg accgctggcc ggtggagcgg ccgtccggac gggtcgcgca
     2341 ggccgggccg gccgggcaac cggcgctgtc ggggtcgtca cgatgtccgg ctcctcctcg
     2401 gcccgattgt cgccgcgtga gcgaccaccg ccgccgccga gcccgcgcac ttcgggcgcc
     2461 ttcagggcgc ggacgcggcc gctcttcagg ccgtcgatcc ggacggggcc gatcctgacc
     2521 cggacgagcc gctcgatcgg
//
LOCUS       DATAIW010000251         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_44724, whole genome shotgun sequence.
ACCESSION   DATAIW010000251 DATAIW010000000
VERSION     DATAIW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44724"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHM48_07810"
     CDS             complement(<1..428)
                     /locus_tag="VHM48_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX2755352.1"
                     /translation="MYIPDHFRPSDDDVAELLANIRAADLVTATADGLLATMLPLIFE
                     APGVSEGAGEHGRLLGHLARNNRQWKVPPLGEAMAIVRGPDAYISPAWYAATREHGRV
                     VPTWNYITAHVHGRLVVHDDPAWVEANVRALSDRHEAARA"
     gene            502..588
                     /locus_tag="VHM48_07815"
     tRNA            502..588
                     /locus_tag="VHM48_07815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:536..538,aa:Leu,seq:taa)
     gene            complement(608..1174)
                     /locus_tag="VHM48_07820"
     CDS             complement(608..1174)
                     /locus_tag="VHM48_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2755353.1"
                     /translation="MGRLIYSMSVSLDGFVETPSRSLDWVQVDDELHAFFNEDARVLS
                     GFLYGRRMYELMADYWPTAEADPSATPTMVDFARIWKATPKIVFSSTLDRVDWNSRLV
                     RDDAVAEVARLKAQPGFDMDVGGPTLAGALMRAGLIDEVRLFLQPVVLGTGLPFFPPL
                     EDRIGLKLLETRTFGSGVVLVRYETISA"
     gene            complement(1365..2588)
                     /locus_tag="VHM48_07825"
     CDS             complement(1365..2588)
                     /locus_tag="VHM48_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755354.1"
                     /translation="MPASTILLISADPAAGETISSVLTGVGYTVTTSTEPADAFAKVT
                     ANQLVIVDVVSGDKSAADVCREIRATPSMVAIPVLCVSQTDEVEERIHFLEAGADDVM
                     AKPFDARELEARVEALLLRFQRSKDRSNVVSADGITVSRTKRVVVVHSPKGGVGTSTI
                     ATNIAMAAAQRKPDRVVIVDLDLQFGQVATHLNIDPRQTLADVVRDEAALREAELLRT
                     YSTKHDSGLHVLSAPTGPELADTITADHVDRILTTLLETYDQVVIDTSSWIDERTLRA
                     FEHAETVLFVVTPEIAALKAMTALVEYLSEAGTVAGKTTFVLNNTFGREILKARDVEQ
                     ALGTKVGAELPYDPFLYLKAVNEGVPIVLGAPRSIAAERLVKLSRTAFGADGIVVPTT
                     AEDRKQGGRFSFRRR"
BASE COUNT          408 a          871 c          981 g          413 t
ORIGIN      
        1 ttcgcccgag ccgcctcgtg gcgatcgctg agggcccgga cgttggcctc gacccaggcc
       61 gggtcgtcgt ggacgaccag ccgaccatgg acgtgggccg tgatgtagtt ccacgtcggg
      121 acgacccgcc cgtgctcgcg cgtcgcggcg taccaggccg gcgagatgta ggcgtcaggc
      181 ccgcgcacga tggccattgc ctcgccgagc ggcgggacct tccattggcg gttgttccgg
      241 gcaagatggc caagcaggcg accgtgctcg ccggccccct ccgacacgcc cggcgcctcg
      301 aagatcagcg ggagcatcgt cgcgagcagc ccgtcggccg tggccgtgac gaggtcggcc
      361 gcgcggatgt tggcgaggag ctcagcgacg tcgtcgtcgc tcggccggaa gtggtcgggg
      421 atgtacacgc cgccagtatc gcgccaccgg cgcgcgggcg gctcccgtca agggctgcgg
      481 ccgggcgtta ggctgacgca ggccggagtg gcggaacagg cagacgcggc ggtcttaaac
      541 accgctgatc gcaagatcgt ccgggttcga accccggctc cggcaccaga agtctcgctt
      601 gtcggtgtca agcgctgatc gtctcgtagc gcaccaagac gacgcctgac ccgaaggtcc
      661 gggtctcgag cagcttcagc ccgatccgat cctcgagcgg cggaaagaac ggcaagccgg
      721 tgccgagaac cacgggctgc aggaacaggc ggacctcgtc gatcagtccg gcccgcatca
      781 acgcaccggc gagggtcggg ccgccgacgt ccatgtcgaa cccgggctgg gccttgagcc
      841 gggcaacctc cgcgacggcg tcgtcacgga cgaggcggct gttccagtcg acccggtcga
      901 gcgtcgacga gaacacgatc ttcggcgtcg ccttccagat ccgggcgaag tcgaccatcg
      961 tcggcgtcgc ggacggatcg gcctcggccg tcggccagta gtcggccatg agctcgtaca
     1021 tccgccggcc gtacaggaag ccgctcagga cgcgcgcgtc ctcgttgaag aacgcgtgca
     1081 gctcgtcgtc gacctggacc cagtcgagcg accggctcgg ggtctcgacg aagccgtcca
     1141 gcgacaccga catcgagtag atgagtctgc ccacggccgg aatcgtaccg cgctccgggc
     1201 cgcgcctgat cggtgtcgtc gatcagatcg gaggcggcca cgctccgcgg ctgcgcagat
     1261 cggtcgggat cacgtcgcgg cgggtggccg gccacgctcg gccaggcgga aaccgatcaa
     1321 cgacggcgat cgcgggcggg ggcgtgccgc tcgctcgccg ccggtcagcg gcggcggaac
     1381 gagaagcgac caccctgctt gcggtcctcg gccgtggtcg ggaccacgat cccgtcggct
     1441 ccgaacgccg tgcgcgacag cttcaccagc cgttcagcgg cgatggatcg tggcgcgccg
     1501 aggacgatcg gcacaccctc gttgacggcc ttgaggtaga ggaacgggtc gtacggcagc
     1561 tccgccccga ccttggttcc gagggcctgc tcgacatcgc gggccttcag gatctcccga
     1621 ccgaatgtgt tgttcaggac gaaggtcgtc ttcccggcga cggtgcctgc ctcgctcagg
     1681 tactcgacga gggcagtcat cgccttgagc gcggcgatct ccggggtgac gacgaagagg
     1741 acggtctcgg cgtgctcgaa ggcgcgcagg gtccgctcgt cgatccacga gctcgtgtcg
     1801 atcacgacct ggtcgtaggt ctcgaggagc gtcgtcagga tccggtcgac gtgatccgcg
     1861 gtgatcgtgt cggcgagctc ggggccggtc ggcgccgaca ggacgtgcag gccgctgtcg
     1921 tgcttggttg aatacgtgcg cagcagctcg gcctcgcgga gcgcggcttc gtcgcggacc
     1981 acgtcggcga gggtctggcg tgggtcgatg ttgaggtgcg tggcgacctg gccgaactgg
     2041 aggtcgagat cgacgatgac gacgcggtcc ggcttgcgct gcgccgcggc catcgcgatg
     2101 ttcgtcgcga tcgtcgacgt cccgacgccg cccttggggc tgtggacgac gacgacccgt
     2161 ttggtccgcg agacggtgat cccgtcggcc gaaacgacgt tgctccggtc cttcgagcgc
     2221 tggaagcgaa ggaggagtgc ctcgacgcga gcctcgagct cgcgggcgtc gaacggcttg
     2281 gccatgacgt cgtccgcgcc ggcctcgagg aagtggatcc gctcctcgac ctcgtcggtc
     2341 tggctgacgc agagaacggg gatcgcgacc atcgacgggg tcgcgcggat ctcgcggcag
     2401 acatcggccg ccgacttgtc gccgctgacg acgtcgacga tgacgagctg attggcggtg
     2461 accttggcga acgcgtcggc cggttcggtc gaggtcgtga ccgtgtagcc gacaccggtc
     2521 aggacggacg agatcgtttc gcccgctgcg ggatcggccg agatgagcag gatggtgctt
     2581 gcgggcacgg atcctccggg gggcgggatg gtccgatgat cgatttggca accgatccgg
     2641 agaccggtgg ccgagaggat acatcgggcg ggc
//
LOCUS       DATAIW010000252        41809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_125515, whole genome shotgun sequence.
ACCESSION   DATAIW010000252 DATAIW010000000
VERSION     DATAIW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 41809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41809
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125515"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VHM48_07830"
     CDS             complement(<1..334)
                     /locus_tag="VHM48_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2755355.1"
                     /translation="MSESSKKSPSLPPRWFIRSFWVVQRAAYSVTGGRLGLRTATTDR
                     WGMLRLRTVGRRSGEERTAILGYFEDGPDLVTMAMNGWADPEPAWWLNLQAHPDVTVD
                     LPGGSRAVH"
     gene            complement(403..627)
                     /locus_tag="VHM48_07835"
     CDS             complement(403..627)
                     /locus_tag="VHM48_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755356.1"
                     /translation="MVKPTLVTNSIRSLRFANGEMTQADLADRVGVTRQTIIAIEQGK
                     YSPTLEMAFRIARVFRVPLDEAFQYADEQE"
     gene            complement(621..1049)
                     /locus_tag="VHM48_07840"
     CDS             complement(621..1049)
                     /locus_tag="VHM48_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755357.1"
                     /translation="MTFLEKSNWVVLVVALPTLLVYVAVVAPQVLGKPIAEVSWVQPM
                     IFAIVGFIVANVLGNVVAAASNPSEADKNDERDREIDHVGERVGNWLIIAGSIAALVL
                     AMTRADHFWIANAIFLGGIAGALLSSVTKIAAYHGSFQRW"
     gene            complement(1277..2242)
                     /locus_tag="VHM48_07845"
     CDS             complement(1277..2242)
                     /locus_tag="VHM48_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2755358.1"
                     /translation="MLGIWRLQLLREVARRGTLKAAAQAMSITPSAVSQQLRILEQEA
                     GAKLLEPHGRLVRLTDAGRMLVRHADTITAAIAAAEADLAATRSEVVGTLSVAAIPTA
                     ARAIMPRVLTALGLSHPKLKLMLRGLESSESLLALQMDEVDLAIVDEYDEATQVHAQG
                     IETIEILRDPLYVALSPASDLPVAGVALADLRDQSWIMDNETSTIFQVTQRACRRAGF
                     DPQVRSHCKDYSVILALVGAGLGVGVVPGLALYDRVIRARVATLQPPLERIVVAAIRS
                     ERRSHPAVVALLAELAAFASAYGNPDSDGSASGLPPAIEDRRILA"
     gene            complement(2296..4677)
                     /locus_tag="VHM48_07850"
     CDS             complement(2296..4677)
                     /locus_tag="VHM48_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX2755359.1"
                     /translation="MRATLDPAVDRFAAAEADFDRYQVLLDIVDECLDVTLNERQSGH
                     PGGSRSKGHLLLATLLSGAMRWDVTRPWRRFADRFVLSAGHTVPLVYATLAVLNEGLR
                     ARHEATGDAAFAFPDEGRWALTWEDLRTFRHRGGLPGHAEMAGKTLLFKANTGPSGHG
                     MPFAAGQALALRMAGADDVKVFVVEGEGGLTPGSTHETLNSAFGLGLSNLILLIDWND
                     FGIDERAASTVIHGTPEDWVAPHGWRVVGTPDGMSWGPVTRALLDVAHGDNPEGLPSA
                     AWFKTRKGRGYGKLDAASHGTPHGLNSPAFWAGRSPFMARYGVTYEGVDRPAPSDSAE
                     RAAQAEANLRIAISVLRRDPALVTWLSDRLVAIAATVPDELPGVHLGRPDSAAIFADP
                     RFTDARAYPEAMFKAPGDRTPNRAALGTWGAWVNAIAKQDYGRPLFVVASADLAESTN
                     IAGFGKDFGDAPGFGWYERDANPTGVVLPQEITEFANAGIIAGMASVNFAEDPFADFD
                     GFWGACSTYASFSYLKYGPMRLFSQMAQDTELGLGKVLWVAGHSGPETAEDSRTHFGI
                     FETGVTQLFPNGAVIDLHPWEHNEVPVMLAAALATDVPIVALHLTRPPVEIPDRAALG
                     MPSHFEAARGAYVLRAYRPDQPRMGTVFVRGTVTTANLVTVLPELDVRNLNVKVVAAL
                     SPQLFARQDASYREATASTADRWDAMAITNGAFRLMADWVEGPAAREYSLAADWDDRW
                     RTGGSMDEVADEAHLSAGHVLAAIERFATERDARRASLRSISEAIGSGAGGVL"
     gene            complement(4674..5858)
                     /locus_tag="VHM48_07855"
     CDS             complement(4674..5858)
                     /locus_tag="VHM48_07855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2755360.1"
                     /translation="MRLASRMEQIGTETAFEAAARAKALEATGREVIHLEIGEPDFDT
                     PSNIRDAAKRALDAGATHYAPYPGIPDLRTAIAEDFSRRRGMAVAPASVFVTVGGKGV
                     MYYAIMALVEAGDEVIVPDPGYPIYASVTRFAGGTPVPIPIRQENDFRLDIDELASLV
                     TAKTRMIVLNSPANPTGGVLTRDDLERVAAIAREHDLVVLSDEIYSRILYEGEHVSIA
                     TLPGMAERTIVLDGFSKTYAMTGWRLGYAILPESLALAFGRLIINSVSSATTFAQVGA
                     VEALRGPQDAVDAMVAEFRARRDLVVDGLNSLPGITCLRPKGAFYVFPDVSGTGRTGD
                     EIADMLLHDAGVSVLSGTAFGEVGTKHIRLSYANSRDAISRAIDKMRTALEPVAAGSR
                     RR"
     gene            complement(5985..7544)
                     /locus_tag="VHM48_07860"
     CDS             complement(5985..7544)
                     /locus_tag="VHM48_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755361.1"
                     /translation="MSAIKRIEMRAIVKRFPGVLANDHVDFDLNAGEVHALLGENGAG
                     KSTLVRQLYGLYRPDEGEILIDGQPVVFHSPSDAIRAGIGMIHQHFMLVPTLTVAENV
                     ALGLKSSRGFRLDLDVVADRIAELSRTYRLKVDPSAYVWQLAVGEQQRVEIIKALYRG
                     ASVIILDEPTAVLTPQEVDDLFITLRRMASEGHALTFISHKLHEVIAISDRVTALRDG
                     RMAGTRPTAGATREELAKMMVGREVRALAPQPMNSRDVRLRIRDLHAIGDRGAEALRG
                     VSLDVSGGQIVGLAGVSGNGQRELAQCLAGLRKVTAGQIEIETRDMTQSSLNERIEAG
                     LAYVPEERMRDGAIREFSVQENVFLHEHADPRFTHGIFLDFGAMKAHARELVTRFSIK
                     TPSLDTPLKNLSGGNIQKVILARELWRKPRILIAAQPTRGVDIGATEYIHERLLEQRD
                     AGTAILLISEDLDEIQELSDTIAVMYEGQIVGTVVRGSASPETLGLLMAGVVPGETRP
                     GRGPAPTVAAG"
     gene            complement(7648..8706)
                     /locus_tag="VHM48_07865"
     CDS             complement(7648..8706)
                     /locus_tag="VHM48_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEX2755362.1"
                     /translation="MKIRKLLLVAAALPIIVAACGTPAASAPSVAPQSASPAAATASQ
                     SAAPPAAKLFRVALLMPSSTTDLAFSQSMYQALVAMQGKAGGQANFEIKFTENMFSVP
                     DAQAAIRDYASQGFDLVVAHGSQYGAGVEEIAKDFPKVSFAWGTDVNTFGLPNVFAYT
                     AAAEQGGYVNGVLAATLTKSRTIGVTGPVEVGDAKTYIAGFSQGVAAVDPAIKISKTW
                     TGSFSDVAAMTEAAKAHITAGADVLTGSSQSVVGSIGAAKEAGNVLWFGTQADQASLA
                     PKLVVASQVYDWTVILGDMIPKIQSGTLGGETYTLQLSNGGLKIAFNPGYALSAEAKA
                     VADKAIADITSGAVKVTP"
     gene            complement(8795..9724)
                     /locus_tag="VHM48_07870"
     CDS             complement(8795..9724)
                     /locus_tag="VHM48_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755363.1"
                     /translation="MNEAVLATVVAVLAASLRLATPYLYASLGEMFAQRSGVLNLGVD
                     GIMLVGAFVAFYGTLTTGSVWVGVAGAMVAGLAMGLLMSVVSVTLKAEQGISGIGLSL
                     FGLGLSSLLFKVLVGTVKTVDGFRPIRLPLLGDIPYLGEIFFTQSPPVYVAFLLVPIS
                     WYVLERTPWGLTVRAVGQNPAAADTLGVNVTLTRYVCVCLGAVFAGLAGASLSIASVN
                     LFQENLTAGAGYIAVALVYFGGWRPVGILMGALLFGTVTAIQLRMQVLGIAIPSDFAV
                     MLPYVLTIVVLTFSANRARQPAALNQPFERGGA"
     gene            complement(9721..10872)
                     /locus_tag="VHM48_07875"
     CDS             complement(9721..10872)
                     /locus_tag="VHM48_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2755364.1"
                     /translation="MATGTRLPSGVRNGRGRDVLGTVLRTALPLVAVAAALLVGAVML
                     AVLGADPVRAYGALFEGAFGSTSGITQTLVKATPLLLVGLGICIAFRASVINIGAEGQ
                     IILGAVFATWFALAFRTWPGWLLIPSTIVIGFVAGAFWGFVPGILKARMGVNEILTTV
                     MMNAIALQFMNYMIRGPLIDPAGVTAGQYLAQSEKLPEQVWLARLIPQTLLHAGIFVA
                     LALAIVTYVFLWRTTIGYRIRAVGLNPDASRYAGIRVPRYQALSLTLAGGLAGVAGAI
                     EVIGVQHRLLDGVTSGYGFSGIVTALFGGLHPLGTIPASFIFGGLLVGADKMQREVQV
                     PSSLIQAILGLIVLFVVASQYWARRRSNRTNDAPTTPAAAALGAGDGQA"
     gene            complement(10980..11927)
                     /locus_tag="VHM48_07880"
     CDS             complement(10980..11927)
                     /locus_tag="VHM48_07880"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX2755365.1"
                     /translation="MTSPLMVVAIGGNALIKDKQHQSVPDQMAALEETSHHLAALVED
                     GWRMVIGHGNGPQVGFILRRSELAAHELHEVPLDVCGADTQGAIGYELQQSLGHDFAR
                     LGLDRQAVTVVTQVQVDLADPAFAAPSKPIGSFLDDETAARRQAEGATIVEDAGRGKR
                     VVVASPKPMRIVELPAVRTLAAADFIVITVGGGGIPVATDAQGNLHGVPAVIDKDFAS
                     SLLASELGADLFVIATAVDRVALDFGKPEQRWLDRMTLAEAKQYLAEGTHFAAGSMAP
                     KIEACIWYLERGGREAVITDPPHLREAVRGTAGTHIVQE"
     gene            complement(11995..12972)
                     /locus_tag="VHM48_07885"
     CDS             complement(11995..12972)
                     /locus_tag="VHM48_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2755366.1"
                     /translation="MQTGLRGRDLISLQEWKTEEVETILEVAARLKLDRALGRPHKYL
                     EDKVLAMLFFFSSTRTRASFEAGMAQLGGHAQFIESRTTQISHGDTAREIGEILGRYN
                     DGLAIRNVDWGVGNKYMRDVAAASRVPVLNMQCDLFHPHQILADLLTMREKKGELRGR
                     TLTVSWAYAASYQKPMSVPIDLVLAATRFGMNVRLVHPPEYKLVPEYVDQAAENARLS
                     GGSLELMDDFDAGFAGSDVIYAKSWGPLLVAKNDEEGATIGAQYTDWITDERRMALAA
                     DDSIFMHPLPADRNVEVTDAVIDGPHSVVYDEAENRLHVQKAVMALTMH"
     gene            complement(13006..13959)
                     /gene="pyrB"
                     /locus_tag="VHM48_07890"
     CDS             complement(13006..13959)
                     /gene="pyrB"
                     /locus_tag="VHM48_07890"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX2755367.1"
                     /translation="MRSFLGRDILSLKDFSRDEFFRVFQVADDLRPFARDRRNTDLLK
                     NKTMLTAFYQPSTRTRLSHEAAMHRLGGQVLGFSDSKMTRAGDFYQESIKDTFHMLEY
                     YADVIAIRHFQQGAPAEAAKWSSVPVINCGDGWGEHPTQVLTDLYTTFLEKGTLDGLT
                     WVLVGDMRMRTMHSILYALSQFDAKAIVVGPPDMSLLPEFKAEIDALNVDYEEASDVR
                     DVIGEADVIYMEPVVQADYTASREERGDKVGLTPAEYCVTRELLKDKAKRSSIILHSL
                     PRMDELLADVDATRHQRYWAEAFNGVVLKMGMLALILGAAE"
     gene            complement(13956..15353)
                     /locus_tag="VHM48_07895"
     CDS             complement(13956..15353)
                     /locus_tag="VHM48_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2755368.1"
                     /translation="MTAQPEVRGRPATEGRFPTWFACSGCGTRPADGAPVPLVCPASV
                     AGDGIDHVLVRHLDPAAGPFAVTDDPNPFVAYRRLFHAWHAARAAGWSDEDYVAMVER
                     LDNAVAEVDGHGFRVTPFARNDALSVALGLSQAGGVWVKDETGNVSGSHKARHLFGTL
                     LELEVGAGATADAPLAIASCGNAALAAAVVARAADRRLLVFIPTDADPAVVTRLRSLG
                     AELTVCDREPGVAGDPTYLRLLEAVAEGAVPFTCQGNLNAFAVEGGETLGFEMASAVG
                     QLDGPSRIDRVVIQVGGGALASSVIAGFANAGEATMTLNQPQFDTVQTRGAFPLARAH
                     ERVAARVTSGETIEAALAFAANHRPEFMWPWEETPHSIAHGILDDETYDWRAVVAGML
                     RTGGAPIVVDEATLHAANDLARASTGIDVDHTGSSGLAGLLHLARSGALDPDENVVVC
                     FSGITRHLEKERTRS"
     gene            complement(15350..16849)
                     /locus_tag="VHM48_07900"
     CDS             complement(15350..16849)
                     /locus_tag="VHM48_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2755369.1"
                     /translation="MGQQGLERDVVDSAVYERAAMRFRDAKIRMPTFAQLADPGTIPA
                     DVEAGLAGVDPDAPDARNLFRVHWYNDADRRSRAGVPQHLVLPTSLTGVAAPIIIALG
                     DRFPMIAAHKVLAAYACLAPRIVTGQFDPIEQRAIWPSTGNYCRGGVAISRIMGCRGV
                     AVLPEGMSQERFDWLEQWVTDPSDIIRTPGTESNVKEIYDKCAELAADSANVILNQFA
                     EFGNHLGHYLVTGRALEAIVEDQRTAQPNITAAAFVSATGSAGTIAAGDWLKDRYGTK
                     IAAVEALECPTLLENGFGEHNIQGIGDKHVPLIHNVMNTDLVIAISDVATDQVLSVFN
                     TDAGRTYLAERRGVPAALIAELGSFGISSICNILAAIKTAKVLDLGPDEAVVTVATDG
                     AAMYLSEQAKITARDFAGGFDAVAAAETFGRHVLGAGEDHLLELTRADRDRIFNLGYF
                     TWVEQQGTPLAEFEARRSQDWWRDLRSLIPAWDEMIDEFNDRTGASSLS"
     gene            17096..19795
                     /gene="xdh"
                     /locus_tag="VHM48_07905"
     CDS             17096..19795
                     /gene="xdh"
                     /locus_tag="VHM48_07905"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HEX2755370.1"
                     /translation="MTTRTIGCDRAALEFAASEGREPMEISFSLNGQPVSVAVRDEER
                     LIDVLRDRFGLRSMKDGCAPEGSCGACTVLVDGRAAVSCAQDAGRVQGRAVVTLEGLP
                     EATRDLFAGAFVAAGAAQCGYCTPGIVMKAEATLNREPAPTREALARSLAGNLCRCTG
                     YVKVLDAIETVAAVRRGEVPLPTAAVEAGVGARADRYDGRDLVLGEFPFVRDMTVPGM
                     LHGAVTLAAHPRAVVKRIDTSRAEQVPGVIRVATWRDVPGQRNQGLIFPDWPVLVAEG
                     ETTRYVGDVLAAVAADTRHAAREAAALVEVEYEVLSPVVNVMAALAEDAPRVHAAGNL
                     LATTEIRIGDVDAALAGAAHVVSETFRTQAVEHAFLEPEACLAVPEPGDDASSRLRPR
                     LRIFSQGQGVWEDRHQIASLLGLEEPEVRVTQVATGGGFGGKEDLSVQGHAALLAFLT
                     GRPVHLALSRAESLRLHPKRHPLVMDYTAGCDAEGNLLGVRARIVGDTGAYASVGDKV
                     LERAAGHACSAYRVPTVDIEARTVYTNNPPCGAFRGFGAVQVAFAMEGILDLLAEKVG
                     IDGWEIRWRNALEVGDRFGTGQKLGPGVGLKKTLLAVRDAYRSAPFAGIACGAKNVGV
                     GNGMRESGRAILRPEADGTVTLYHSWTEMGQGIHTVLRQILVDELGVSADQVRIIVDT
                     ERELNTGETTASRATTLGGQAVIDAARRLRAEAGDRPLSQFEGREFYGEYVVDWTTKS
                     NVPEPITHFAYGWATQVVILDEDGRLERVIAAQDVGRVMNPTAMEGQVEGGIHMGLGM
                     ALSEDLGLRDGMPVGTLKQLGIIPPSGMPPVECILVEEHQPEGPYGAKGAGEAVLVPT
                     PGAVAGALFAYDGIHRTTLPMRDTVAARAAAPNVARLLARAQA"
     gene            19821..20843
                     /locus_tag="VHM48_07910"
     CDS             19821..20843
                     /locus_tag="VHM48_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2755371.1"
                     /translation="MTATTPGLVCAHHHLYSALARGMPAPPNVARNFQEILEQVWWRL
                     DTALDLEMIRWSAMLGALEALESGTTAIVDHHESPNAIEGSLDVIADACAEVGVRSLV
                     AYGITDRHGADGARRGLAENERFIRAGGRGLVGIHAAFTCSDESLASAAELAEDLGVG
                     VHIHVCEGIGDIDAPRRLAGLSRDDWLLAHCVHLPTDHELRGTILHNPMSNLNNAVGY
                     ADPARFSNPVALGTDGIGGNVLESFRLAYVLQRSVDVTSSPDTAWVWLGTGWDLVPEA
                     REDTVTWSYDPMDPWHIAFTPGIRPLEITVGGEVVLRDGRPTRVDADEIRAKAREQAT
                     RLHARL"
     gene            20902..24312
                     /locus_tag="VHM48_07915"
     CDS             20902..24312
                     /locus_tag="VHM48_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2755372.1"
                     /translation="MADRFHPISMEQLTHWVFTELEQKDSLFNVPRSAFFVPRPEHRY
                     RVQESGVELETPFGVAAGPHSQMAQNIVAAWLVGARVIELKTIQTLDELDVNKPCIDV
                     TDEGYNVEWSQELRVHESFDEYLRAWVLIHALHRRFGWPGETPGVLFNMSVGYNLEGI
                     LKPNVQWYFDAMADASAWLPQYVDIVARHEPAVRDLAIPARLSNTITLSTMHGCPPDE
                     IEKISEYLLVDKGLHTSVKCNPTLLGPEGVRQIVNADLGFTDVPIPDAAFGHDLKYAD
                     AVPMFRRLQGVAAGRGLGFGLKLSNTLEVENRRTVFDRDPTMYLSGRPLHAVTTNLAQ
                     RLTEEFEGGLALSFAGGADCFNVADLLRSGMRTITVASDLLKTGGYLRLLQYTEKTDE
                     AFDAVGATSTADFIVRSAATAGLPGVAGTNPATVAAAARFNLRTYADAVRRDWRYRKD
                     SFQMDRSKTPRGLGAFDCIEPPCVDKCPIDQAVPLYMGAVRSGDIAEAIRITRTDNPL
                     PSILGRACDHLCETTCIRTHLDQPLAIRHIKRFIMDQEAIADARVGAGPPPTVRVAII
                     GAGPAGLAAARDLARDGIGVTIFEAQPYAGGMVGGAIPEYRLPQARLDQDLVPLERLG
                     VEFRYGQRAGVDFTLDQLQADGFDAIFVAVGAQVAKHLNLPGDDADGIIDGVTFLRGV
                     REGRPIAVGAHVGVIGAGDTAMDCARSARRLGAETVSLIYRRTVDQMPADPEEILESA
                     EEGVAFVEHASPAGLHVEDGRLAGLVCTRTEYRGERDASGRKVPFDVPDSEFEIGLET
                     LIVAISQHSMLDFFGDRPPRLTRAGFIVVDPVTFETSIPGVFAGGDVAAHGPSSIVRA
                     AADGKRVAAAIAAVHGMTRPVAATAAEPIDIHGLVVRRARREYRVPISVVPVEDRMGF
                     TETTLGYTQEEAMVEASRCLDCHQICSLCVGVCPNMALMTYESEPIATRLPALTVRGG
                     AVVAGDAQRPFAATQRLQIAVLTDFCNECGNCVTACPTSGSPYRDKPRLFLDRADFLA
                     QESNAFMVLGSGLMEGRFEGETHRIAVDGRIDYRAPTFSAMLEPASLELIEATPNGAR
                     EGDDLSLEPAALMATLLAGLRRSMPHLPVAATDGTFVREPEPPVRPGA"
     gene            24922..26298
                     /locus_tag="VHM48_07920"
     CDS             24922..26298
                     /locus_tag="VHM48_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX2755373.1"
                     /translation="MNAGQPERALFHWTLKREGIVAPDERLPWPQTIALGVQHIFAMF
                     GATVLAPILMGFDPNTAILFSGIGTLIFFVIVGGRIPSYLGSSFSFIAVVIAATQYSG
                     SGANPNLGVALGGIIAAGALYALIGVIVMAVGYRWIEVLMPPVVTGAVVAVIGLNLAP
                     VAISNLVSSDAAAPQSVSQFATFVGLLTVVAVGIVAVRVPGLFRRLPILLGGIFGYVA
                     YLVLANGLGWGKAIDFTALGNAAWFGAPNLTAPSFEPSALTLIAPVAIILVAENLGHV
                     KAVAAMTGRNLDPYLGRAFLGDGVATMIAGAGGGTGVTTYAENIGVMAVTKIYSTLVF
                     VIAGVVAIAMGFSPKFGALIGTIPAPVLGGLSIVLFGMIAATGARIWIQNKVDFSNNR
                     NLITAAVALTLGAGNLAITIGDFQLGGIGTATFGAIIVYQLLREPRSTVSVETTPLVE
                     PTGAATPA"
     gene            26534..27706
                     /locus_tag="VHM48_07925"
     CDS             26534..27706
                     /locus_tag="VHM48_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX2755374.1"
                     /translation="MARQDGLYAALLERGVTRRDFLKFSAAMSAALALPAGYAPRIAA
                     AVTSAPRVPVIWLEGQDCAGNTEAFLRASHPTVAELVLDTLSVDYHETIMAAAGQQAE
                     AARLATMKAFPHGYIAVVEGSVPVADGGVYCTIGGKAFSATVREVCDGALATIAVGSC
                     AFDGGLPAAAGGTTGAIGAGQAVPGAKIINLPGCPMNVQNLTATIVHFLTFKQWPATD
                     VLDRPLFAYGQLIHDQCERRAHFERGEYVQAWGDDASRKGWCLYQMGCKGPETFANCP
                     TARFNDGTSWPVKAGHGCVGCTMPNFWDQMSPFYRRLPTVGPFATDVTADQVGLGLVG
                     VVTALTAVHAAGSAVRKRRTEARVRAELAAVSAPVTAGTTPQSTPPDEQTEKDGQA"
     gene            27703..29412
                     /locus_tag="VHM48_07930"
     CDS             27703..29412
                     /locus_tag="VHM48_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2755375.1"
                     /translation="MTRIAVDPITRIEGHLRIEVEVANGVVTDAWSSGTMFRGLELIL
                     KGRDPREAWVFAQRACGVCTTVHALASVRSVENALGLRIPRNARLVRNLIAGAQYVQD
                     HVIHFYHLHALDWVDVVSALKADPDATSRLAQSLSDHPLSSAAYFKGVRDRLNTFVQS
                     GQLGPFANGYWGHPAYKLPPEANLLAVAHYLEALDWQRQVIRIHALLGSKNPHPQTYA
                     VGGMALSLDPNSPTALNARGLSEIAELIAEAKRFVDEVYIPDVLAIAGFYKDWAAIGK
                     GIGNYMSYGEFPEDDSGAEAAFYLPRGRIMGRDLGKVESVDQIGVTETVDHSYYTYDG
                     GDATPLHPWSGQTHPKYAGPTPPFETLEGHDKYSWLKAPRYQDEPMEVGPLARMLVAY
                     GSGQPDVKAAVDGVLGKLGVGPEALFSTLGRIAARAIETQLIVHRLERWLTDLTDNLA
                     TGDLAIADTTKWDPATWPAEAQGWGFEEAPRGALGHWVRIKDRAIDNYQLVVPSTWNG
                     SPRDPKGRRGAWEEALVGTPVADPNQPVEILRTVHSFDPCMACAVHVYDPKAGSSVQI
                     RVV"
     gene            29423..30262
                     /gene="cybH"
                     /locus_tag="VHM48_07935"
     CDS             29423..30262
                     /gene="cybH"
                     /locus_tag="VHM48_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HEX2755376.1"
                     /translation="MTTPVVTADPIVRTVPAPARPVEEKLPPTMERVRIYVWQVPVRL
                     THWVTFGTLIALAVTGSYIADPFLITPGQDTMRLVRFVHIVAAYSFVASGIVRTYWLF
                     AGNQFSRWTAFVPTNRHHLRELARQTQWYLFLRRDLPGILGHNALASGTYLVVFFLFL
                     LQTATGFALVGIHGTQPWATLLGWLPGVLFGEQGVRLIHHLLMWAIIAFAIHHVYSAL
                     LVDHIERNGLLSSIFSGSKFVPRWRIVEARDGGIELDSIVRRQDIEESRAEALDKAMR
                     GEE"
     gene            30259..30765
                     /locus_tag="VHM48_07940"
     CDS             30259..30765
                     /locus_tag="VHM48_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX2755377.1"
                     /translation="MSGPATPLVVGIGNVLLRDEGVGVAVVRELERRAAEGRLDLPPG
                     TRLVDGGTLGLDLLPLLSSASMLVLVDAVDLGRPPGTVCVIRDAAIEGTLAGHVSPHQ
                     VGVADLVAAARLIGVFPATASLVGIQPALIEIGLELSDEVEAAVGEAMERVCAELHRG
                     PDAAAAAA"
     gene            30765..31103
                     /locus_tag="VHM48_07945"
     CDS             30765..31103
                     /locus_tag="VHM48_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HEX2755378.1"
                     /translation="MHEAGLAAEIAVRLQEGRRTGRAGRPRVLVRGGHDASADFDASL
                     RFHLALVAPGLSAGLEIVHLPVARLCASCGRGFEDAEVFATCPSCGGAALPVSTGEEA
                     VVEWSPEMAG"
     gene            31108..31404
                     /locus_tag="VHM48_07950"
     CDS             31108..31404
                     /locus_tag="VHM48_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2755379.1"
                     /translation="MCVAFPGRVVEVDDVGAVVDTDGRLRRVSTLFLPDVAVGDWVAV
                     MADTIVERLEEAHALEVQQILRDAIALEVAEENGQPRADEMPVPGAPRVTTGTI"
     gene            31502..32554
                     /gene="mgrA"
                     /locus_tag="VHM48_07955"
     CDS             31502..32554
                     /gene="mgrA"
                     /locus_tag="VHM48_07955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX2755380.1"
                     /translation="MTDLPQTERPPFVAAPTRYDAMPYRRVGRSGLDLPAISLGLWQN
                     FGENRPLAVQRAIVRLAFDRGITHFDLANNYGPPFGAAEETFGRIYASDLRPYRDELV
                     ISTKAGYDMWSGPYGDRGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDPATPMEETLG
                     ALDTAVRSGKALYAGISSYSADRTRKAAAILAGLGTPLLIHQPSYSMLNRWIEGGLLD
                     VLGETGVGCIAFTPLAQGLLTDRYLDGVQAGSRVTAKDSFAPEFLSEANLAKVRALNE
                     IAKARGQSLAQMAIAWILRDPRVTSAVVGASSVEQLTDTLGALDQLDFSPDELAAIDR
                     HATESGVNLWAASSDA"
     gene            32724..32897
                     /locus_tag="VHM48_07960"
     CDS             32724..32897
                     /locus_tag="VHM48_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755381.1"
                     /translation="MDAHLEMGESNITYEDVKRAADENGRSVAETLDIMERTKAKDRD
                     DHPQEYATPNAGS"
     gene            complement(32894..33445)
                     /locus_tag="VHM48_07965"
     CDS             complement(32894..33445)
                     /locus_tag="VHM48_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755382.1"
                     /translation="MPIPSGTIATGRLELVPLRIADADEMVAVLADPGLYAFIGGGPP
                     THDELVARFEAQVRGRSPDGREEWHNWVVRERPSGAATGFVQATVVDVGGAGAGRGAE
                     IAWLIGVPWQGRGYASEAARGLVAWLEAAGVRSILAHVHPDHLGSAAVAERAGLAPTD
                     ELVDGERVWRRIVRASAGTGAAD"
     gene            complement(33489..34250)
                     /locus_tag="VHM48_07970"
     CDS             complement(33489..34250)
                     /locus_tag="VHM48_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755383.1"
                     /translation="MTSVEHVDAKGFEIERIRSAIAGLGLSPERLQATAEHLGTMAES
                     RGATFRERLLAMTRDRTETRPKRRMPHGLTVALRTAGMVWAALMLSTLAVAIASRSTA
                     RKTMAALQPEADEIHVRTALGPMAFTSRAAAFRGGVIDCWYGGGFVDLREATLHPDGA
                     VLRVRAVFGGGQIVVPESWRISARVRGIGGLQDVRTAADLAADAPHLTIVGLALFGGF
                     AVQSDLPEEEIAELEKAVARMSGGAPLAVEAVPTV"
     gene            complement(34506..37034)
                     /locus_tag="VHM48_07975"
     CDS             complement(34506..37034)
                     /locus_tag="VHM48_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2755384.1"
                     /translation="MTSSRAVPPRVGARPAADPQRGLSTAEAEARTLAGEANEFHTPT
                     SRSYLEIVRDNSYPFINGPLLLVSLALIGFGATTEAVMTGLPVFLNIAIGVVQGSRAK
                     RQLDRVALLSEAPATVVRDGVERQVPPAAVVLGDVVVANRGDEIQLDGRIVGDATASV
                     DESALTGESNPIHKSAGDTVQSGSAVLSGVARYEVEAVGADTYANRILAQAKGRRVVR
                     TPLQSEIARTFIVVGALIALAAGVVVLTLPTAITDAAQETVFAAAVLITLVPQGLALM
                     LTVTYAAAALRISRLGALTQRPSAIEAISRIDTFCTDKTGTLTTQQITFRDLRRLVTA
                     GMSTADLERVVAAVAASGTTPNRTTYALATAFPGPGLAVAEEIPFSSALRWSAIRFAA
                     GDAHHPASAGTWLLGAPSVLAPAMRDRADEVRSRATASAETGQRVLVLARASAGAALA
                     VDDQPRLPDRLEPIALLTFEEELRPEAPATIDGLRARGIDVKIVSGDDPVTVQAIGRR
                     LGLGGGTNAASGLDLADLGDDELADLAIRTTIFGRVDPHLKARLVTVLKRRGRFVAMT
                     GDGVNDILPVRIADVGVAMQSGSPATRGVADLVLVRDDFSILPEAIRDGQRIVAAMAA
                     TLTVLLARTFYVLLIIVGAALLQLPFPFTPRQNSILALVTVGVPILVLALWVRPVPSR
                     SGLLGQTLRISIPLSLGVAAVGLPVYADAIAIGAPTDVARTMLTTMATFLGIGALVLI
                     PIAADDSGRIRMPARVRTALLVGGLVVAYLAVLATDAGRDIFQLSALPIGIHLSLGLI
                     ALAWTGAVVRIHRTRIVARVNDRLVLGWRRFVTRRPAGAADASP"
     gene            complement(37176..37952)
                     /locus_tag="VHM48_07980"
     CDS             complement(37176..37952)
                     /locus_tag="VHM48_07980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2755385.1"
                     /translation="METVISKDGTKLAVDVQGSGPAIVLVSGGSVDRGSNAGLADALK
                     ADFTTYNYDRRGRGDSGDTTPYAVEREIEDIEAVIALAGGKAHLYGSSSGAGLAFEAA
                     AAGAPVDKLVLWEPPYNLDPAGRPPADSVEQLDRLVAEGRRGDAATFFMVEMVRLPAD
                     FAAFAKTQPWWAAQEAIAHTLAYDARVMRDYSVPTATAARVTVPTLILTGGASFPFFR
                     PTAEALVAALPDGRTAVLEGQRHDVDATVLGAAMAAFLRS"
     gene            complement(38000..38737)
                     /locus_tag="VHM48_07985"
     CDS             complement(38000..38737)
                     /locus_tag="VHM48_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2755386.1"
                     /translation="MAHPFEVRVERTVNATPEQVWEAVSTAAGLDAWFMGENDVEPRL
                     GGAVRTTLHGFTMDSTVTTWDPPHRFANTSPEADDGRLMTFAFEIEGRAGGTTTLRFV
                     HSGFLPGDDWEQEYDALKIGDPAYVAKLVEYIEYFRGRIAVPVTLWGPQVSRQRAWAA
                     FTDALGVTREPVVGQPAHFRAEGLPDLAGEIDYVSPDFIGLRTADGMYRFIHGMGTAA
                     LGHHIFQPVDREQTEAAWQAWVDRTFA"
     gene            complement(38739..39368)
                     /locus_tag="VHM48_07990"
     CDS             complement(38739..39368)
                     /locus_tag="VHM48_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2755387.1"
                     /translation="MLDVTVIENAAAAAASLDPVRSRLLAELSTPGSATTLALKVGLP
                     RQQVNYHLRTLERHGLVELVEERRKGNMTERLMRATAASYVISPAALAAVQPDPERSP
                     DRLSAFWMLALAARLVRDVGSLLIGARKAGKRIATFALDGEIRFASAADRSAFAEELS
                     HAVAALVARYHDESAAGGRPHRIVVAIHPSVPAATDLGAPVTPEIEEES"
     gene            complement(39429..39851)
                     /locus_tag="VHM48_07995"
     CDS             complement(39429..39851)
                     /locus_tag="VHM48_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX2755388.1"
                     /translation="MTPTPIVLAVYGTLRRGERNHAFLAGSTFLGRGSVLGRLFEMPR
                     SAARAYAYPALVLGGPSRVVVELYDIPDPAALAAADDLEAFDPADEAGSQYVRRVVGI
                     VDGPVAAAWVYLYNGPPADMGEVIPDGDWVTFLARGGA"
     gene            39875..40138
                     /locus_tag="VHM48_08000"
     CDS             39875..40138
                     /locus_tag="VHM48_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755389.1"
                     /translation="MSAGRIVTGTSPVDLRALGKPAAGPHAVRAGRARATPEGRLVGG
                     VDIDPMLLYAVITIAFLVLGILALGYGVDSRDGFEDDDWRRRS"
     gene            40236..40697
                     /locus_tag="VHM48_08005"
     CDS             40236..40697
                     /locus_tag="VHM48_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755390.1"
                     /translation="MTDWNAFAAGAPSIAAAGRRLIERSGIGEGLLATVRGDAPPRIH
                     PVHVRIVDGRLLTFVVVGSAKSADLEADGRYALHAHQDPAVPHEFLVRGLATEVRDAD
                     LRATAAGEWSFEVDDGYRLYELSIDHAVFGERATADDWPPVYTAWRAGPSA"
     gene            40768..>41809
                     /locus_tag="VHM48_08010"
     CDS             40768..>41809
                     /locus_tag="VHM48_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755391.1"
                     /translation="MIDPSSLRRRAPSIDRSSDRLVAVIAIGAAVSFLIAAVLAMFLP
                     VVDRRGAWLPIHLALAGGATTAIAGVMPFFAAAFAAAPPSDARLRMSAVLAVAAGAIG
                     VSVGVVGSQPDVAVRGGVAFIAGVVVTGVATIRPLARALGPSRGLVSQGYVVALAEVA
                     VGAILATLYVGGWAPMLAAWGQVKPAHAWLNLVGFVSLVVATTLLHFFPTVVGARITV
                     RPSARLTVVGLAVGAPVVALGFAIGSDLLARIGALASAAGAIGLAVNAARTWQTRARW
                     TTDPGWHRFAMGALVAAITWFVVGIAIAVGRVLVAGASPAAFSIETVGAPLVAGWMGL
                     AILGSATHLLPAV"
BASE COUNT         6185 a        14547 c        14847 g         6230 t
ORIGIN      
        1 cgtggacggc gcgcgaccca ccgggcagat cgacggtgac gtcgggatgc gcctgcaggt
       61 tgagccacca ggcgggctcg ggatcggccc agccgttcat ggccatggtg accaggtcgg
      121 gaccgtcctc gaagtagccg aggatcgccg tccgctcctc gccgctgcgg cgcccgaccg
      181 tccgcaggcg cagcatgccc cagcggtcgg tcgtcgcggt ccgcaggcca agccggccgc
      241 ctgtgaccga ataggcggca cgctgcacca cccagaacga gcggatgaac cagcgcggcg
      301 gcagagacgg tgacttcttc gacgattcgg acatggtgtg ctcccgatcg gtgtgagggt
      361 ggttgtgacg tccggcggag gccggggctg gcggtcggct ggtcattcct gttcgtccgc
      421 gtactggaag gcctcgtcga gcggcacgcg gaagacccgg gcgatccgga aggccatctc
      481 gagcgtgggc gagtacttgc cctgctcgat ggcgatgatc gtctggcggg tcacgccgac
      541 tcgatcggcc aggtccgcct gggtcatctc gccgttggcg aacctgagcg agcggatcga
      601 gttggtgacg agggtcggct tcaccatcgc tggaacgacc cgtggtaggc cgcgatcttg
      661 gtgacggagc tgagaagggc tcccgcaatg cccccaagga agatcgcgtt ggcgatccag
      721 aagtggtccg ccctggtcat cgccaggacg agcgcggcga tcgagccggc gatgatcagc
      781 cagttgccga cccgctcacc gacgtggtcg atctccctgt cgcgctcgtc gttcttgtcc
      841 gcctcgctgg ggttcgaggc cgcggccacg acgttgccca ggacgttcgc cacgatgaag
      901 ccgacgattg cgaagatcat cggctggacc caggagacct cggcgatggg cttgccgagc
      961 acctgcggcg cgaccacggc cacgtagacg aggagcgtcg gcagcgcgac gacgagcacg
     1021 acccagttgc tcttctccag gaatgtcatc ggcgtctccc atgtcaagaa ttgttgacat
     1081 gagcaatgta caagatcgcc gacatattgt caatgatctt tgacattggt ccttgcgctc
     1141 accacgaggg tcccggctcg caggaacgtg gcgggtctgc tgcgagaacg caccgaacgt
     1201 cgcgtcggaa cggcgcgaga acgtgcggaa gggcctcggc ggtctccggc gcggcggtct
     1261 ccggcgcccg ggtccgctac gcgaggatcc gtcggtcctc gatcgccggc ggcagtcccg
     1321 aggcagagcc atcgctgtcc ggattcccgt acgcggaggc gaaggccgcc agttccgcca
     1381 gcagcgccac aaccgcggga tgggaccgcc gttccgagcg gatcgcggca acgacgatcc
     1441 gctcgagggg cggctgcagc gtggcgacgc gcgcgcggat gacgcggtcg tacagcgcga
     1501 ggcccgggac gacgccgact ccgagcccgg ccccgacgag ggcaaggatc accgagtagt
     1561 ccttgcagtg cgagcgcacc tgtggatcga acccggctcg ccggcacgcg cgctgggtga
     1621 cctggaagat cgtgctcgtc tcgttgtcca tgatccacga ttgatcgcgg aggtcggcga
     1681 gggcgacccc cgccacgggc aggtcggaag cgggcgagag cgcgacgtac agcggatcgc
     1741 gaaggatctc gatcgtctcg atcccctggg cgtgcacctg tgtcgcctcg tcgtattcgt
     1801 cgacgatcgc gaggtcgacc tcgtccatct ggagcgcgag caggctctcg ctggattcga
     1861 ggccgcgcaa catcagcttg agcttcgggt ggctgagccc gagtgcggtg aggacccgcg
     1921 gcatgatcgc ccgggcagcg gtcgggatgg cggcgacgct gagcgtcccg acgacttcgc
     1981 tgcgggtcgc ggcgaggtcg gcttcggcgg cggcgatggc cgcggtgatc gtgtcggcgt
     2041 ggcggacgag catccggccg gcatcggtca ggcggacgag tcgtccatgc ggttcgagca
     2101 gcttcgcacc ggcttcctgc tcgaggatcc ggagctgctg cgagaccgcg gatggcgtga
     2161 tcgacatcgc ctgggcggcc gccttcaggg tgcctcgtcg ggcgacttcg cggaggagtt
     2221 gcagacgcca gatcccgagc atcaaccgtt ccgatccgac tcgtatgcgt tcacgaatcg
     2281 ttccatccgc tgagcctaca gcacgcctcc cgcaccggac ccgatcgcct cgctgatcga
     2341 gcggagcgat gcgcgtcggg catcgcgttc ggtcgcgaag cgctcgatcg cggccaggac
     2401 gtgcccggcc gacaggtgcg cctcgtcggc gacctcgtcc atcgagccac ccgttcgcca
     2461 gcggtcgtcc cagtcggcgg ccagcgaata ctcgcgtgcc gccggaccct caacccagtc
     2521 ggccatcagg cggaaggccc cgttcgtgat cgccatcgcg tcccatcggt cggccgtcga
     2581 ggccgtcgct tcccggtacg aagcgtcctg gcgcgcgaac agctgcgggc tcagggcagc
     2641 gaccaccttg acgttcaggt ttcgcacgtc gagctcgggc aggaccgtga ccaggttggc
     2701 cgtcgtcacc gtgccacgaa cgaagaccgt tcccatccgc ggctggtccg gccgataggc
     2761 gcggagcacg tacgcacccc gggcggcctc gaagtgcgac ggcatcccga gggcagcccg
     2821 gtcggggatc tcgaccggtg gccgggtgag gtgcagggcc acgatcggca cgtcggtcgc
     2881 gagcgcggcc gcaagcatca cggggacctc gttgtgctcc catgggtgca ggtcgatgac
     2941 cgcgccattc gggaagagct gggtgacccc ggtctcgaag atgccgaagt gggtgcgcga
     3001 atcctcggcg gtctcggggc ccgagtggcc cgccacccaa aggacctttc cgagcccgag
     3061 ctcggtgtcc tgggccatct gcgagaagag gcgcatcggg ccgtacttca ggtagctgaa
     3121 cgaggcatag gtcgagcacg cgccccagaa gccgtcgaag tcggcgaacg ggtcctccgc
     3181 gaagttgact gacgccatcc cggcgatgat cccggcattc gcgaactcgg tgatctcctg
     3241 cggcaggacg acacccgtcg ggttcgcgtc gcgctcgtac cagccgaacc ccggcgcgtc
     3301 gccgaagtcc ttgccgaacc cggcgatgtt cgtcgattcg gccaggtcag ccgaggcgac
     3361 cacgaagagc ggccgcccgt agtcctgctt cgcgatcgcg ttgacccacg cgccccaggt
     3421 gcccagggcg gcgcggttcg gcgtgcgatc gcccggcgcc ttgaacatgg cctccgggta
     3481 agcccgggca tcggtgaacc gggggtccgc gaagatcgcg gcactgtcgg gccgcccgag
     3541 gtggacgccg gggagctcgt cggggacggt ggcggcgatc gcgacgaggc gatcggagag
     3601 ccacgtcacg agcgccgggt cccggcggag gacggagatt gcgatgcgca ggtttgcctc
     3661 ggcctgggcc gcgcgctccg cggaatcgct cggggcgggc cggtccacgc cctcgtaagt
     3721 gaccccgtag cgcgccatga acgggctgcg gccagcccag aaggcggggc tgttgaggcc
     3781 gtgcggcgtg ccgtgcgagg ccgcgtccag cttgccgtac ccacggccct tgcgcgtctt
     3841 gaaccaggct gcggatggaa gcccctcagg attgtcgccg tgggccacgt cgaggagcgc
     3901 gcgggtcacc ggtccccagg acatcccgtc cggggtcccg acgacgcgcc acccatgcgg
     3961 cgccacccag tcctcgggcg tgccgtggat caccgtcgac gccgcccgct cgtcgatccc
     4021 gaagtcgttc cagtcgatca gcaggatgag gttcgagagg ccgagcccga aggccgagtt
     4081 gagcgtctcg tgcgtggagc cgggcgtcag accgccctcg ccctcgacca cgaagacctt
     4141 gacgtcgtcg gcgccggcca tccggagcgc gagcgcctgc cccgccgcga acggcatccc
     4201 gtggcccgac ggcccggtgt tcgccttgaa caagagcgtc ttgccggcca tctcggcatg
     4261 cccgggcagg ccgccacgat gacggaacgt gcgcaggtcc tcccaggtca gcgcccaccg
     4321 gccctcgtcc ggaaaggcga aggcagcgtc gcccgtggcc tcgtgccggg cacggaggcc
     4381 ctcgttgagc acggcgagcg tcgcgtacac gagcgggacc gtgtgaccgg cggacaggac
     4441 gaaccggtcc gcgaagcggc gccacggccg ggtgacgtcc cagcgcatcg caccggacag
     4501 gagcgtggcg aggaggagat ggcctttcga gcgcgagccg ccgggatgtc ccgactggcg
     4561 ctcgttgagg gtcacgtcga ggcactcgtc gacgatgtca agaaggacct ggtagcggtc
     4621 gaagtcggcc tcggcggccg cgaagcggtc gaccgccgga tcaagcgttg ccctcatcgt
     4681 cgtcggcttc cggccgcgac gggctcgagg gcggtccgca tcttgtcgat cgcgcggctg
     4741 atcgcgtcgc ggctgttcgc gtaggaaagg cggatgtgct tcgtaccgac ctcaccgaat
     4801 gccgttcccg acaggaccga gacgccggcg tcgtggagca gcatgtccgc gatctcgtca
     4861 cccgtccggc ctgtccccga gacatcggga aagacgtaga aggcgccctt tgggcggagg
     4921 catgtgatgc ccgggagcga gttgaggccg tcgacgacga ggtcgcgccg agcacggaac
     4981 tcggcgacca tggcgtcgac cgcgtcctgc gggccgcgca acgcctcgac ggcgccgacc
     5041 tgcgcgaacg tcgtggccga gctgaccgag ttgatgatca gccgcccaaa ggcaagggcg
     5101 agcgattcgg gcaggatcgc gtagccgagc cgccagccgg tcatggcata ggtcttggag
     5161 aaaccgtcga ggacgatcgt ccgctcggcc atgcctggca aggtcgcgat ggagacgtgc
     5221 tcgccctcgt agaggatccg actgtagatc tcgtcggaca ggaccacgag gtcgtgctcc
     5281 cgcgcgatgg ccgcgacgcg ctcgaggtcg tctcgggtga gcaccccgcc ggtcgggttg
     5341 gccggcgagt tgaggacgat catcctggtc ttcgccgtga cgagcgaggc gagctcgtcg
     5401 atgtcgagcc ggaagtcgtt ctcctggcgg atcgggatgg ggaccggcgt gccgccggcg
     5461 aaccgggtga cgctggcgta gatcgggtac ccggggtcgg ggacgatcac ctcgtcgcct
     5521 gcttcgacca gggccatgat cgcgtagtac atgacgccct tgccgcccac ggtcacgaat
     5581 acgctggcgg gcgcgacagc catgccgcgc cgcctcgaga agtcctcggc gatcgccgtc
     5641 cgcaggtccg ggattcccgg gtacggggcg taatgcgtgg cgcccgcatc gagcgcgcgc
     5701 ttggcggcat cgcggatgtt cgaaggcgtg tcgaagtcgg gctcgccgat ctccaggtgg
     5761 atcacctcgc ggccggtggc ctcgagtgcc ttggcgcgag cggccgcctc gaaggccgtc
     5821 tcggttccga tctgttccat gcgcgacgcg agccgcatcg attgctcctt ctcatcaggt
     5881 tgcaccggtg ccggagcgcg tgaatcgagc gggcgctccg cgcgctgaca gtgcgcgctc
     5941 atgatcagtt acgtcaagtg aattgttctt tgataatcaa ttagctaacc tgctgctacg
     6001 gtcggcgccg ggccgcggcc cggtcgagtc tcgccgggaa cgacacccgc catcaggagc
     6061 cccagggttt cgggcgatgc gctgccgcgg accacggtcc cgacgatttg cccctcgtac
     6121 atgaccgcga tcgtgtcgga gagctcctgg atctcgtcga ggtcctcgga gatcagcagg
     6181 atcgccgtgc ccgcgtcgcg ctgctcgagg agccgctcgt ggatgtattc ggtcgcgccg
     6241 atgtccacgc cacgggtggg ctgggccgcg atgaggatcc tcggcttgcg ccacagttcg
     6301 cgggccagga tcaccttctg gatgtttccg ccggacaggt tcttgagcgg ggtgtcgagc
     6361 gagggcgtct tgatcgagaa ccgcgtcacg agctcgcgcg cgtgggcctt catggcgccg
     6421 aagtcgagga agatgccgtg ggtgaaccgt ggatcggcgt gttcgtgcaa gaagacgttc
     6481 tcctgcacgc tgaactcccg gatcgcccca tcccgcatgc gttcctcggg cacgtacgcc
     6541 agccccgcct cgatccgttc gttgagtgac gactgggtca tgtcccgcgt ctcgatctcg
     6601 atctgaccag cggtcacctt gcgcaggccg gccaggcact gggcgagctc gcgctggccg
     6661 ttccccgaca cgccggcgag gccgacgatc tggccaccgg acacgtccag gctgacgccc
     6721 cgcagggcct ccgccccccg gtcgccgatc gcatggaggt cgcggatccg gagccggaca
     6781 tcgcggctgt tcatcggctg cggggcgagc gccctgacct cacgcccgac catcatcttg
     6841 gccagctcct cgcgggtggc gcccgcggtg gggcgtgtgc cggccatccg cccgtcgcgg
     6901 agggcggtca cgcgatcgct gatcgcaatc acctcgtgca gcttgtgcga gatgaatgtg
     6961 agcgcgtgtc cctcgctcgc catgcgcctc agggtgatga acaggtcgtc gacctcctgg
     7021 ggcgtgagca cggccgtcgg ctcgtcgagg atgatcacgc tggcgccgcg gtagagcgcc
     7081 ttgatgatct cgacgcgctg ctgctcgccg accgcgagct gccagacata ggccgacgga
     7141 tcgaccttga ggcggtaggt gcgggacagc tcggcgatcc gatcggccac cacgtcgagg
     7201 tccaggcgga acccccggga cgacttgagc cccagggcga cgttctccgc cacggtcagt
     7261 gtcgggacga gcatgaagtg ttgatggatc atcccgatcc cggcccggat ggcgtccgat
     7321 ggggaatgga agaccaccgg ttggccgtcg atcaggatct cgccctcgtc gggccggtag
     7381 agcccgtaaa gctggcggac gagcgtgctc ttgcccgccc cgttctcacc gagcagcgcg
     7441 tggacctcac ccgcgttgag gtcgaaatcg acgtgatcgt tggcgaggac gcccggaaag
     7501 cgcttgacga tggcccgcat ctcgatgcgc ttgattgcac tcatgggtct gcttgcgtcc
     7561 gttgtcatcg actggcgata tgaggcttgg cgggcccggg ggcgggcgat gcatgggggc
     7621 actcccgcgc cccgggcccg gtgcgcgtta cggggtgacc ttcaccgcgc cgctcgtgat
     7681 gtccgcgatg gccttgtcgg caaccgcctt ggcctcggcg gacagggcgt aacctgggtt
     7741 gaacgcgatc ttgagaccgc cgttcgagag ctggagggtg taggtctcac caccgagcgt
     7801 gcccgactgg atcttgggga tcatgtcgcc gaggatcacg gtccagtcgt acacctgcga
     7861 ggcgacgacc agcttgggcg caagcgatgc ctggtcggcc tgggtgccga accagagcac
     7921 gttcccggct tccttggcgg ccccgatcga gccaacgacc gactgtgacg agcccgtcag
     7981 cacgtccgcg ccggcggtga tgtgggcctt ggcggcctcg gtcatcgctg cgacgtccga
     8041 gaacgatccg gtccaggtct tggagatctt gatcgccggg tcgaccgcag cgacgccttg
     8101 gctgaagcca gcgatgtagg tcttggcatc gccgacctcg accgggcccg tgacgccgat
     8161 cgtgcgcgat ttcgtgaggg tggcggcgag gacgccattg acgtatccgc cctgctcggc
     8221 ggcggcggtg taggcgaaga cgttcggcag gccgaaggtg ttcacgtcgg tgccccacgc
     8281 gaacgaaacc ttcgggaagt ccttggcgat ctcctcgacg ccggcgccgt actgcgagcc
     8341 gtgcgccacg accaggtcga acccctgcga cgcatagtcg cgaatcgcgg cctgcgcgtc
     8401 gggcacgctg aacatgttct cggtgaactt gatctcgaag ttcgcttggc cgccggcctt
     8461 gccctgcatg gcgacgagcg cctggtacat cgactggctg aaggccaggt cggtcgtgga
     8521 gctcggcatg aggagggcga cgcggaacag cttggccgcc ggcggtgcgg ccgattggga
     8581 tgcagtcgct gccgcaggtg acgccgattg gggcgcgacg gagggagcgg aggccgcggg
     8641 cgtgccgcat gccgccacga tgattggcag cgcggctgcc acgaggagca gtttccggat
     8701 cttcatgggt tccttcttgc gaatcaggtc gcatggtctg ccagggtgtt ggctgggtct
     8761 cgcgatgtcg atgattgcct cccgggcccg ggtctcaggc ccctccgcgt tcgaatggtt
     8821 ggttgagcgc cgccggctgg cgggcccggt tggcggaaaa ggtgaggacg acgatcgtca
     8881 ggacgtacgg cagcatcacg gcgaagtcgg acgggatcgc gatgcccagg acctgcatcc
     8941 ggagctggat cgcggtgacc gtgccgaaca ggagggcgcc catgagaatc ccgacgggcc
     9001 gccagccgcc gaagtagacg agcgcgaccg cgatatagcc ggccccggct gtcaggttct
     9061 cctggaagag gttgaccgag gcgatcgaga gcgacgcccc ggccaggccg gcgaacaccg
     9121 cgccgaggca gacgcacacg tagcgcgtga gcgtgacgtt gacgccgagc gtgtccgcgg
     9181 cggccgggtt ctgcccgacg gcgcggacgg tgaggcccca cggcgtccgt tccaggacgt
     9241 accagctgat cggcacgagc aggaaggcga cgtagaccgg cgggctctgg gtgaagaaga
     9301 tctcgccgag gtacgggatg tcgccgagca gcgggaggcg gatcggccgg aagccatcga
     9361 ccgtcttgac ggtgccgacg aggaccttga agagcaggct cgaaagcccg agcccgaaga
     9421 gcgagagccc gatcccgctg atgccctgct ccgccttgag agtgacgctg acgaccgaca
     9481 tcagcaggcc catggccaga cccgcgacca tcgcaccggc cacgccgacc cagacgctgc
     9541 cggtggtcag cgtcccgtag aaggcgacga atgccccgac gagcatgatc ccgtcgacgc
     9601 caaggttgag gaccccggac cgctgggcga acatctcgcc cagggatgca tacaggtacg
     9661 gggtcgccag ccggagtgag gcggccagga cggccacgac ggtggcgagg acggcctcgt
     9721 tcatgcctgg ccgtctccag cgccgagggc ggccgccgcc ggcgtcgtcg gggcgtcgtt
     9781 cgtgcggttc gatcgccgtc gcgcccagta ctgcgaggcc acgacgaaca gcacgatcag
     9841 gccgaggatc gcctggatga gcgacgaggg cacctggacc tcgcgctgca tcttgtcggc
     9901 gccgaccagc aggcccccga agatgaagga ggccgggatc gtcccgagcg ggtggagccc
     9961 gccgaacagg gcggtcacga tcccgctgaa gccgtagccg ctcgtgacgc cgtcgagcag
    10021 gcggtgctgc acgccgatca cctcgatcgc gccggccacg cccgcgagcc cgccggccag
    10081 cgtgagcgac agcgcctgat agcgcggcac ccggatcccc gcgtagcgcg acgcgtccgg
    10141 gttcaacccg accgcgcgga tccggtagcc gatcgtcgtt cgccacagga agacgtaggt
    10201 cacgatcgcc aaggcgagcg cgacaaagat ccccgcgtgg agcagggtct gcggaatgag
    10261 ccgggcgagc cagacctgtt cgggcagctt ttccgattgg gccagatact ggccggccgt
    10321 gacgccggca ggatcgatca gcggcccgcg gatcatgtag ttcatgaact ggagtgcgat
    10381 cgcgttcatc atcacggtcg tgaggatctc gttgaccccc atccgggcct tgaggatccc
    10441 cggcacgaag ccccagaacg ccccggccac gaagccgatg acgatcgtgg acgggatcag
    10501 cagccagccc ggccaggtgc ggaacgcgag tgcgaaccag gtcgcgaaga ccgccccgag
    10561 gatgatctgg ccctccgcgc cgatgttgat cacgctggcc cggaaggcga tgcagatccc
    10621 gagccccacg aggaggaggg gcgtggcctt gaccaatgtc tgggtgatcc ccgacgtgct
    10681 cccgaacgcg ccctcgaaca gcgcgccgta ggcccggacc gggtcggcgc ccagcacggc
    10741 gagcatcacc gcgccgacga gcagtgccgc cgcgacggct acgagcggga gcgccgtccg
    10801 caggaccgtc ccgaggacgt cgcggccgcg accgttgcgc acgccggacg gcaagcgggt
    10861 gccggtggcc acgcggctac cgacccacca actgggtcac cgccgggcgc tccaccatcg
    10921 tgcgatccgg accgtgtccg tccgcgggcg aacgtgcggc accgacgact tcatgccggt
    10981 cactcctgga cgatgtgggt cccggcggtg ccgcggaccg cctcgcggag gtgcggcggg
    11041 tcggtgatga cggcttcgcg gccgccgcgt tcgaggtacc agatgcaggc ctcgatcttg
    11101 ggcgccatgc tgccggccgc gaagtgggtg ccctcggcga ggtactgctt cgcctcggcc
    11161 agggtcatcc tgtccagcca gcgctgctcg ggctttccga agtcgagcgc gacccggtcc
    11221 accgcggtgg cgatcacgaa caggtcggcc ccaagctcgc tcgccaggag cgagctggcg
    11281 aagtccttgt cgatcacggc gggcacgcca tggaggttgc cctgggcatc ggtggcgacc
    11341 gggatcccgc caccgccgac cgtgatcacg atgaagtcgg ctgcggcgag ggtccggacg
    11401 gctggcagct cgacgatccg catgggcttc ggcgacgcga cgacgacgcg cttgccccgt
    11461 cccgcgtcct cgacgatcgt cgcgccctcg gcctggcgcc gggccgccgt ctcgtcgtcg
    11521 aggaacgacc cgatcggctt cgagggcgcc gcgaaggccg ggtcggccag gtcgacctgc
    11581 acctgcgtca ccacggtcac ggcctgccga tcgagcccga gccgggcgaa atcatggccg
    11641 agcgactgct ggagctcata gccgatcgcg ccctgcgtgt cggcgccgca cacgtcgagc
    11701 ggcacctcgt ggagctcgtg tgcggccagc tcggaacggc ggaggatgaa cccgacctgc
    11761 ggcccgttgc cgtggccgat gaccatgcgc cagccgtcct cgacgagcgc ggccaggtga
    11821 tggctcgtct cttcaagcgc ggccatctga tcgggcaccg actggtgctg cttgtccttg
    11881 atcagcgcgt tgccgccgat cgccacgacc atgagcgggc tcgtcatcgc cattctcccc
    11941 ggcacgtcgg ccggtctgct gctgatggtc cctcgccctg ggcggttacc cgcctcagtg
    12001 catcgtgagc gccatcacgg ccttctggac atggagccgg ttctcggctt cgtcgtacac
    12061 gaccgagtgc ggaccgtcga tgaccgcgtc ggtcacctcg acgttccggt cggccgggag
    12121 gggatgcatg aagatggagt cgtccgccgc gagcgccatt cgccgttcgt cggtgatcca
    12181 gtcggtgtac tgcgcgccga tcgtcgcgcc ctcctcgtcg ttcttggcga cgaggagcgg
    12241 accccagctc ttggcgtaga tgacatcgga gcccgcgaag ccggcatcga agtcatccat
    12301 cagctcgagg ctgccgcccg acagccgggc gttctcggcg gcctggtcga cgtactccgg
    12361 gacgagtttg tactcgggcg gatggaccag ccggacgttc atgccgaacc gggtcgcggc
    12421 caggacgagg tcgatcggga cgctcatcgg cttctggtag ctggccgcgt acgcccagct
    12481 gacggtcagc gtccggccgc ggagctcgcc cttcttctcc cgcatcgtca gcaggtcggc
    12541 gaggatctgg tgtggatgga agaggtcgca ctgcatgttg aggacgggga cgcgactggc
    12601 cgcggccacg tcgcgcatgt acttgttgcc gacgccccag tcgacgtttc ggatggcaag
    12661 cccgtcgttg tagcgaccga ggatctcgcc gatctcgcgc gccgtgtcac cgtgcgagat
    12721 ctgggtcgtc cgggattcga tgaactgggc gtgcccgcca agctgtgcca tccccgcctc
    12781 gaacgacgcg cgggtgcgcg tgctcgagaa gaagaacagc atcgcgagga ccttgtcctc
    12841 gaggtacttg tgcggccgcc ccagggcgcg gtccagcttc aggcgggccg ccacctcgag
    12901 gattgtctcg acctcctcgg tcttccattc ctggagcgag atgaggtctc ggccacgaag
    12961 gcccgtctgc atggccgtgt tccttccgat tcggggttcc gggggttact cggccgcgcc
    13021 gaggatgagg gcgagcatgc ccatcttcag cacgacgccg ttgaaggctt ccgcccagta
    13081 gcgctggtgg cgggtggcgt ccacgtcggc cagcagctcg tccatccgcg gcagcgagtg
    13141 gaggatgatc gagctgcgct tggccttgtc cttgaggagc tcacgggtca cgcagtactc
    13201 ggccggcgtg aggccgacct tgtcaccgcg ctcctcgcgc gaggcggtgt agtcggcctg
    13261 gacgaccggc tccatgtaga tgacatccgc ctcgccgatg acgtcacgga cgtccgaggc
    13321 ctcctcgtag tccacgttga gcgcgtcgat ctcggccttg aactcgggca ggaggctcat
    13381 gtccgggggg ccgacgacga tcgccttggc gtcgaactgc gacagcgcgt acaggatcga
    13441 atgcatcgtc cgcatccgca tgtcaccgac caggacccag gtgagcccgt cgagcgtgcc
    13501 cttctcgagg aacgtcgtgt acaggtcggt aaggacctgg gtcgggtgct cgccccagcc
    13561 gtcgccgcag ttgatgaccg gcacggacga ccacttggcc gcctcggccg gtgcgccctg
    13621 ctggaaatgc cggatcgcga tgacgtccgc gtagtactcc agcatgtgga acgtgtcctt
    13681 gatcgactcc tgatagaagt cgccggcgcg cgtcatcttc gagtcggaga agccgaggac
    13741 ctgaccaccg agacggtgca tcgcggcctc gtgcgacagg cgcgtccgcg tcgacggctg
    13801 gtagaaagcc gtcagcatcg tcttgttctt gagcaggtcg gtgttccggc ggtcacgcgc
    13861 gaatgggcgg aggtcatccg cgacctggaa gacgcggaag aactcgtccc tgctgaagtc
    13921 cttgagggac aggatgtcgc ggccgaggaa acttctcatg atctggtgcg ctccttctcg
    13981 aggtggcggg tgataccgga aaagcagacg acgacgttct cgtcggggtc gagcgcgccg
    14041 ctgcgcgcga ggtggaggag accggcaagc ccggacgagc cggtgtggtc gacgtcgatc
    14101 ccggtcgacg cgcgggcgag gtcgttggcg gcgtggagcg tcgcctcgtc gacgacgatt
    14161 ggtgcgccgc cggtgcgcag cattccggcc acgacggcgc gccagtcgta cgtctcgtca
    14221 tcgaggattc cgtgggcgat gctgtgcggc gtctcctccc acggccacat gaactcgggg
    14281 cgatggttcg cggcgaaggc gagcgcagcc tcgatggtct cgccgctcgt cacgcgcgcc
    14341 gcaacgcgtt cgtgggcccg tgcgaggggg aacgcgccgc gcgtctggac ggtgtcgaac
    14401 tgcggctggt tgagggtcat ggtggcctcg ccggcgttgg cgaaaccggc gatgaccgac
    14461 gaggcgaggg cgccgccgcc gacctggatc acgacccggt cgatccggga cgggccgtcc
    14521 agctgtccga cggccgaggc catctcgaag ccgagcgtct cgccaccttc gaccgcgaag
    14581 gcattgaggt tcccctggca ggtaaagggc acagcgccct cggcaaccgc ctccaggaga
    14641 cgcaggtagg tcgggtcgcc agccacgccc ggttcccggt cgcagaccgt cagctctgcc
    14701 ccgagggagc ggaggcgcgt gacgaccgcg ggatcggcat cggtcgggat gaagacgagc
    14761 agccggcggt cggccgcgcg cgcgacgacc gcggccgcga gcgccgcgtt gccgcaactc
    14821 gcgatcgcga gcggtgcatc ggcggtcgcg ccagcaccga cctcgagctc gagcagtgtc
    14881 ccgaagaggt gacgggcctt gtgcgatccc gacacgttgc cggtctcgtc cttgacccag
    14941 acgcccccgg cctgcgacag gccgagcgcg acggagaggg cgtcatttcg cgcaaacggc
    15001 gtgacccgga acccgtggcc gtcgacctcg gcgaccgcgt tgtccaaccg ctcgaccatc
    15061 gccacgtagt cctcgtcgct ccaacccgcg gcgcgcgcgg cgtgccaggc gtggaacagg
    15121 cgccggtacg cgacgaacgg gttcgggtcg tcggtgaccg cgaagggtcc ggccgccggg
    15181 tcgaggtgtc ggacgaggac atggtcgatc ccgtcgccgg cgacgctcgc cggacagacg
    15241 agcgggaccg gtgcgccgtc ggccggccgc gtgccgcagc cggagcaggc gaaccaggtt
    15301 gggaaccggc cctcggttgc gggccggccg cggacctcgg gttgggcggt catgacagtg
    15361 agctggcgcc cgttcggtcg ttgaactcgt cgatcatctc gtcccaggca gggatgagag
    15421 accgcaggtc gcgccaccag tcctgggatc ggcgggcctc gaactcggcg agcggcgtgc
    15481 cttgctgttc gacccaggtg aagtatccga ggttgaagat ccggtcgcga tcggcacgcg
    15541 tcagctcgag caggtggtcc tcgcccgcgc ccaggacatg ccgcccgaag gtctcggcgg
    15601 ccgcgacggc atcgaagccg ccggcgaagt cgcgggccgt gatcttggcc tgctccgaga
    15661 ggtacatcgc cgcaccgtcc gtggcgaccg tgacgacggc ctcatcgggg ccgaggtcga
    15721 ggaccttggc agtcttgatc gcggcgagga tgttgcagat gctcgagatg ccgaacgagc
    15781 cgagctccgc gatgagcgcc gccggcacgc cacgccgctc ggcgaggtac gtgcggcccg
    15841 cgtcggtgtt gaagacggac agcacctggt cggtggcgac gtccgagatc gcgatgacca
    15901 ggtccgtgtt catcacgttg tggatgagcg gcacgtgctt gtcgccgatg ccctggatgt
    15961 tgtgctcgcc gaagccgttc tccaggagcg tcgggcactc gagtgcctcg accgcggcga
    16021 tcttcgtccc gtaccggtcc ttgagccagt cccccgccgc gatcgtcccg gccgatccgg
    16081 tcgcggatac gaacgccgcc gccgtgatgt tcggctgggc cgtgcgctgg tcctcgacga
    16141 ttgcctcgag cgcccgaccg gtcacgaggt agtggccgag gtggttcccg aactcggcga
    16201 actggttgag gatcacgttc gcggagtccg cggccagctc agcgcacttg tcgtagatct
    16261 ccttcacgtt gctctcggtg cccggcgtcc ggatgatgtc ggaggggtcc gtcacccact
    16321 gctccagcca gtcgaagcgc tcctggctca tgccctcggg caggaccgcg acgccgcggc
    16381 accccatgat ccggctgatc gccacgccac cgcggcagta gttgccggtg gacggccaga
    16441 tcgcgcgctg ctcgatcggg tcgaactggc cggtgacgat ccgcggcgcc aggcaggcgt
    16501 acgcggcgag aaccttgtgc gcggcgatca tcgggaagcg gtcgccgagg gcgatgatga
    16561 tcggggcggc gacgccggtc agcgaagtcg gcaggaccag gtgctgcgga acgccggccc
    16621 gcgaacggcg gtcggcatcg ttgtaccagt ggacccggaa caggttccgg gcatcgggcg
    16681 catccgggtc gacgccggcg agtccggcct cgacatcggc cggaatcgtg cccggatcgg
    16741 cgagctgcgc gaacgtcggc atccggatct tcgcgtcccg gaaacgcatc gcagcgcgtt
    16801 catagaccgc gctatcgacg acgtcccgct cgagtccctg ctgtcccatc gtccatcctc
    16861 gacaacacca ttggccggga gcggatcagg cccgctcgac gccgaacttg gccgggaacg
    16921 gcggctcatc gcgtgccacg atgcttggca gggatgctac cattccgcca ccgatcctgt
    16981 aaccttaatt taactaacgg cagggaatac gtaaccttat ctgtacctag cgcctcacga
    17041 cgaatcgggc tgcagacgcg aatcgggccg cgcgagaaga tggttcgatc cccggttgac
    17101 gactcgaaca atcggctgcg atcgagccgc gctcgagttc gcggccagcg aaggcaggga
    17161 accgatggag atctcgttct cgctcaacgg ccagcccgtt tcggtcgcgg tgcgcgatga
    17221 agagcgcctg atcgacgtcc tgcgcgaccg gttcggcctc aggtcgatga aggacggctg
    17281 cgccccggag ggctcgtgcg gcgcctgcac ggtgctggtg gacgggaggg ccgcggtctc
    17341 ctgcgcccag gacgccgggc gcgtccaggg tcgcgcggtg gtgacgctcg aagggctgcc
    17401 cgaagcgact cgtgacctct tcgccggggc gttcgtggcg gcgggcgccg cccagtgcgg
    17461 gtactgcacg cccgggatcg tcatgaaggc cgaggcaacg ctgaaccgcg agcccgcccc
    17521 gacgcgcgag gcccttgccc gctcgctcgc gggcaacctg tgccgctgca ccggctacgt
    17581 gaaggtgctc gacgcgatcg agacggtggc ggccgtccga cgcggcgagg tgccgctccc
    17641 gacggctgcg gtcgaggccg gggtcggggc ccgcgcggac cgctacgacg ggcgcgatct
    17701 cgtcctgggc gagttcccgt tcgtccgcga catgacggtc ccgggcatgc tccacggtgc
    17761 cgtcaccctg gcggcccatc cgcgcgcggt cgtgaagcgg attgacacgt cacgtgccga
    17821 acaggttcca ggtgtgatcc gcgtcgccac ctggcgcgat gtcccgggcc agcgcaacca
    17881 gggcctcatc ttcccggact ggccggtact ggtcgccgag ggcgagacga cccgctatgt
    17941 cggcgacgtg ctcgcggccg tcgcagccga cacgcgccat gcggcccgcg aggccgccgc
    18001 gctggtcgag gtggagtacg aggtgctgtc gccggtcgtg aacgtgatgg cggctctcgc
    18061 cgaggacgcg ccccgggtcc acgcggcggg caacctgctc gcgacgaccg agatccggat
    18121 cggcgacgtc gatgccgccc tcgcgggtgc cgcccacgtg gtgagcgaga cgttccgcac
    18181 gcaggccgtc gagcacgcat tcctcgagcc cgaggcctgt ctcgcggttc ccgagccggg
    18241 cgacgacgca agcagccggc tccgcccgcg tctgcggatc ttctcgcagg gtcaaggcgt
    18301 gtgggaggac cggcaccaga tcgcctcgct gctcgggctc gaggagcccg aggtgcgggt
    18361 cacccaggtc gccacgggcg gcgggttcgg cggcaaggag gacctcagcg tccagggtca
    18421 cgccgccctg ctcgcgttcc tgacgggtcg accggtccac ctcgctctgt cccgcgccga
    18481 gagcctgcgg ctccatccga agcgccaccc gctcgtcatg gactacacgg ccggctgcga
    18541 tgccgagggc aacctgttgg gcgtgcgcgc ccggatcgtc ggcgataccg gcgcctacgc
    18601 gagcgtcggc gacaaggtcc tcgaacgggc ggccggccac gcctgctccg cgtaccgcgt
    18661 ccccacagtc gacatcgagg cgcggaccgt ctacacgaac aacccgccgt gcggcgcctt
    18721 ccgcggcttc ggcgcggtcc aggtcgcctt cgcgatggag gggattctcg acctccttgc
    18781 cgagaaggtc ggcatcgatg gctgggagat tcgctggcgc aacgccctcg aggtcgggga
    18841 ccggttcggc acgggccaga agctgggtcc gggcgtcggg ctcaagaaga ccctcctcgc
    18901 ggtccgcgac gcctatcgat ccgcgccgtt cgcggggatc gcctgcggcg ccaagaacgt
    18961 gggcgtgggc aacgggatgc gggagagtgg ccgggcgatc ctccgaccgg aggcggacgg
    19021 gacggtgacc ctgtaccact cgtggaccga gatgggccag ggaatccaca ccgtcctgcg
    19081 ccagatcctc gtcgacgagt tgggcgtctc ggccgaccag gtccggatca tcgtcgacac
    19141 cgagcgcgag ctcaacaccg gtgagacgac cgcctcgcgg gccacgacgc ttggcggaca
    19201 ggccgtgatc gatgcggcgc gccgcctgcg cgccgaggcc ggcgatcgac cgctgagcca
    19261 gttcgagggc cgcgagttct acggcgagta cgtcgttgac tggacgacga agagcaacgt
    19321 ccccgagccg atcacccact tcgcctacgg ctgggcgacc caggtcgtca tcctcgacga
    19381 ggacggccgg ctggagcgtg tgatcgcggc ccaggacgtg ggtcgagtca tgaacccgac
    19441 cgcgatggag ggccaggtcg aaggcggcat ccacatgggc ctcggcatgg ccctgtccga
    19501 ggacctgggg ctgcgcgacg gcatgccggt ggggacgctc aagcagctcg ggatcatccc
    19561 gccgtcgggc atgccgccgg tcgagtgcat cctcgtcgag gagcatcagc ccgagggacc
    19621 ctacggtgcg aagggcgccg gtgaggcggt ccttgtcccg accccgggcg ccgtcgccgg
    19681 cgcactcttt gcctacgacg gcatccatcg caccaccctg ccgatgcgcg acacggtcgc
    19741 ggccagggcc gccgccccga acgttgcccg gctccttgcc cgggcgcagg cctgatccgg
    19801 cccgggagga cgatctggcc atgaccgcaa ccacccccgg cctcgtctgc gcgcaccacc
    19861 atctgtattc ggcgctcgcc cgcggaatgc cggcgccgcc gaacgtggcg aggaatttcc
    19921 aggagatcct cgagcaggtc tggtggcggc tcgacacggc gctcgacctc gagatgatcc
    19981 gctggtcggc gatgctcggc gccctcgagg cgctcgagag cggcaccacc gcgatcgtcg
    20041 atcatcacga gtccccgaac gcgatcgagg gcagcctcga cgtgatcgcg gatgcctgcg
    20101 ccgaggtcgg cgtgcggtcg ctcgtggcct acggcatcac cgatcgccac ggcgccgacg
    20161 gtgctcggcg cggcctggcc gagaacgagc ggttcatccg ggcggggggc cggggcctcg
    20221 tcggcatcca cgccgccttc acctgctccg acgagtcgct ggcctctgcc gccgagctcg
    20281 ccgaggacct gggcgtcggc gtccacatcc acgtctgcga ggggatcggc gacatcgacg
    20341 cgccgcgccg cctcgcgggc ctcagccgcg atgactggct cctggcccat tgcgtccacc
    20401 tgccgaccga ccacgagctc cgcggcacga tcctccacaa cccgatgtcg aacctcaaca
    20461 acgccgtcgg ctacgccgac cccgcccggt tttcgaaccc ggtcgcgctg ggcacggacg
    20521 ggatcggcgg caacgtcctc gagtcgttcc ggctggccta cgtgctgcag cgatcggtcg
    20581 acgtcacgtc gagcccggac acggcctggg tttggctcgg caccggctgg gacctcgttc
    20641 cggaggcccg cgaagacacc gtgacctggt cgtacgaccc gatggacccc tggcacatcg
    20701 cgttcacgcc gggcatccgg cccctcgaga tcaccgtggg cggtgaagtc gtcctccgcg
    20761 atggccgccc gacccgcgtc gatgcagacg agattcgggc gaaggcgcgc gaacaagcca
    20821 cccgcctcca cgcgcgcctc tgaccgtccc cccggcgacg cgccgaccat cacgaaacac
    20881 tccacgacga ggacgaatcc catggccgac cgtttccatc ccatctcgat ggagcagctc
    20941 acgcactggg tcttcaccga gctcgagcag aaggattccc tcttcaacgt cccgcgctcg
    21001 gcgttcttcg taccgcgccc ggagcaccgt taccgggtcc aggaatccgg cgtcgagctc
    21061 gagacgccgt tcggcgtcgc tgccgggccg cattcgcaga tggcccagaa catcgtcgcc
    21121 gcgtggctcg tcggcgcccg cgtcatcgag ctcaagacga tccagacgct cgacgagctt
    21181 gacgtcaaca agccctgcat cgacgtcacc gacgagggct acaacgtcga gtggtcgcag
    21241 gagctgcggg tccacgagtc cttcgacgag tacctccggg catgggtcct gatccacgcg
    21301 ctgcaccggc ggttcggctg gcccggcgag accccgggcg tgctcttcaa catgagcgtt
    21361 ggctacaacc tcgaggggat cctcaagccg aacgtccagt ggtacttcga cgcgatggcg
    21421 gacgcctccg cctggctgcc gcagtacgtc gacatcgttg cccggcacga acccgcagtt
    21481 cgtgacctcg cgatcccggc gcgcctgtcg aacacgatca cgctctcgac gatgcacggc
    21541 tgcccgcccg acgagatcga gaagatctcc gagtacctcc tcgtggacaa ggggctgcac
    21601 acgtcggtca agtgcaatcc cacgctgctc ggtccggagg gcgtccgcca gatcgtcaac
    21661 gccgacctcg gcttcacgga cgtgccgatc cccgatgccg ccttcggcca cgacctcaag
    21721 tacgccgacg ccgtgccgat gttccggcgc ctgcaggggg tcgccgccgg tcggggactc
    21781 ggcttcgggc tcaagctgtc gaacacgctc gaggtcgaga acaggcgaac cgtgttcgac
    21841 cgcgacccga cgatgtacct gtccgggcgc ccgttgcacg cggtcacgac gaacctggcc
    21901 cagcggctga cggaggaatt cgaaggcgga ctggcgctgt cgttcgcggg cggcgccgac
    21961 tgtttcaacg tcgcggacct gctgcgctcg ggcatgcgca cgatcacggt cgcctcggac
    22021 ctgctcaaga ccggcggcta cctccggctg ctccagtaca ccgagaagac cgatgaggcg
    22081 ttcgacgccg tcggggcgac ctcgaccgcc gacttcatcg tccggtcagc ggcaacggcc
    22141 ggcctcccgg gcgtggccgg gaccaacccg gccaccgtgg ccgcggcggc tcgcttcaac
    22201 ctgcgcacgt atgccgacgc ggttcgccgg gactggcgct atcgcaagga ctcgttccag
    22261 atggaccgat ccaagacacc gcgcgggctt ggcgcgttcg actgcatcga gccgccctgc
    22321 gtcgacaaat gcccgatcga ccaggcggtc ccgctctaca tgggcgccgt ccgcagcggc
    22381 gatatcgcgg aagcgatccg gatcacccgc acggacaacc cactgccgtc gattctgggc
    22441 cgggcgtgcg accacctgtg cgagacgacc tgcatccgga cgcacctcga tcagccgctc
    22501 gcgatccgcc acatcaagcg cttcatcatg gaccaggagg ccatcgccga cgcccgggtc
    22561 ggcgccgggc cgcccccgac cgtccgggtc gcgatcatcg gtgcgggtcc cgcaggactg
    22621 gccgccgcac gcgacctggc ccgcgatggg atcggggtga cgatcttcga ggcacagccg
    22681 tatgcgggcg gcatggtcgg cggcgcgatc cccgagtacc ggctcccgca ggcccggctc
    22741 gaccaggacc tcgtgccgct cgagcgcctc ggggtggagt tccggtacgg ccagcgggcg
    22801 ggcgtcgact tcacgctcga ccagctgcag gcggacggct tcgacgcgat cttcgtcgcg
    22861 gtcggggcgc aggtggcgaa gcacctcaac ctgcccggcg acgacgcgga cggcatcatc
    22921 gacggcgtca cgttcctgcg cggcgttcgc gaaggccggc cgatcgcggt cggcgcgcac
    22981 gtcggcgtga tcggcgccgg cgacaccgcg atggactgcg cccgctcggc gcgccgcctc
    23041 ggggcggaga cggtgtccct gatctaccgg cgcacggtcg accagatgcc ggccgacccg
    23101 gaggagatcc tcgagagcgc cgaagagggg gtcgccttcg tcgagcacgc gagccccgcc
    23161 ggcctgcacg tcgaggacgg ccggctggcc ggcctcgtct gcacccggac cgagtaccgc
    23221 ggtgaacgcg acgcctcggg ccggaaggtg ccgttcgacg tcccggactc cgagttcgag
    23281 atcggcctgg aaacgctgat cgttgcgatc agccagcatt cgatgctcga cttcttcgga
    23341 gaccggccgc ccaggctgac ccgcgctggc ttcatcgtcg tcgacccggt caccttcgag
    23401 acctcgatcc cgggggtctt cgccggtggc gacgtggcgg cgcacggacc ctcgtcgatc
    23461 gtgcgagcgg cggccgacgg caagcgtgtc gccgccgcga tcgcggccgt ccacggcatg
    23521 acgcgaccgg ttgccgccac cgcggcggag cccatcgaca tccacgggct ggtcgtccgg
    23581 cgcgcccggc gcgaataccg cgtcccgatc agcgtcgttc ccgtcgagga ccgcatgggc
    23641 ttcacggaga cgacgctcgg gtacacgcag gaagaggcga tggtcgaggc ctcacgctgc
    23701 ctcgactgcc atcagatctg cagcctgtgc gtgggcgtct gcccgaacat ggccctgatg
    23761 acctatgaat cggaaccgat cgcgacgagg ctgccggccc tcacggtgcg cggtggggcg
    23821 gtcgttgccg gcgatgccca acggccgttc gccgcgacgc agcggctgca gatcgcagtc
    23881 ctgaccgact tctgcaacga gtgcgggaac tgtgtgaccg cctgcccgac gtcgggctcc
    23941 ccgtaccgcg acaagccgcg cctgttcctc gaccgcgccg acttcctcgc gcaggaatcg
    24001 aacgcgttca tggtgctcgg cagtgggctg atggagggtc gattcgaggg cgagacccac
    24061 cggatcgcgg tcgacggccg gatcgactac cgcgccccga ccttcagcgc catgctcgag
    24121 cccgcgagcc tggagttgat cgaggccact ccgaacggag cccgcgaggg cgacgacctt
    24181 tcgctcgaac cggccgcgct gatggcgacc ctgctggcgg gtctgcgccg gtcgatgccg
    24241 cacctcccgg tcgccgcgac cgatgggacg ttcgtacgcg aacctgagcc gcccgtcagg
    24301 ccgggagcct gagaccgcga gaggcaattg acacgccccg gcgcaggcgg tcgagctgac
    24361 ggcgctccgc acgcctgaag gcgatcagga tggtgcacct gccttttttc gcgatgttcg
    24421 ccagacaccc ccgttctcgg acggcgcgat gcgtccgaac tgaggtcgac acgacggccc
    24481 gtgaacgtga aatcgagccg acacgggcga atggccaagc gctatagcgc ctgagtctgg
    24541 atcaagcgcg ctcggccatg aggccgtgat ctgttcccgc gatcgggcga cggcgccatc
    24601 gggacggtgt cgttcctgaa actcctgctg gaggcagacg cggggtccaa accggggctg
    24661 tcgccgttgc ctggcgtcgg cgactggccc accccgatgt cgggcacgat cagctggtcg
    24721 tgcggaccgt gggccacccc ggtctcgtcg ggcgcgccgc cgccgtcggt cgctccgtaa
    24781 cgcattcggc cggccttcaa caggccgatc ggcccctccc gtgccatcgg ttttctgaca
    24841 ccctcgcccg aggcgtccca caagacctcc cacgcccggc ctgtgagccg ggtggggcca
    24901 gcaggcgagg agatcgatcg catgaacgcc gggcagcctg aacgcgccct cttccactgg
    24961 accctcaagc gcgaggggat cgtcgcgccc gatgagcggt tgccgtggcc gcagaccatc
    25021 gccctcggag tccagcacat cttcgcgatg ttcggtgcga cggtcctcgc cccgatcctc
    25081 atgggcttcg acccgaatac ggccatcctg ttctcgggga tcgggacgct gatcttcttc
    25141 gtgatcgtcg gcggacggat cccgagctat ctcgggtcca gcttctcgtt catcgccgtc
    25201 gtcatcgcgg cgacccagta ttccggctcc ggcgccaacc cgaacctcgg cgtcgccctc
    25261 ggcgggatca tcgccgcggg cgcgctgtac gccctgatcg gcgtgatcgt catggccgtc
    25321 ggctaccgct ggatcgaggt cctgatgccg ccggtcgtga ccggcgccgt ggtggccgtc
    25381 atcgggctca acctggcccc cgtcgcgatc agcaatctgg tgtcgagcga cgccgccgcc
    25441 ccgcagagcg tcagccagtt cgcgacgttc gtcggcctgc tgacggtcgt cgccgtcggg
    25501 atcgtcgcgg tccgggtgcc cgggctattc cgccgcctgc cgatcctgct gggcgggatc
    25561 ttcggctatg tcgcctacct ggtcctcgcc aacggcctcg gctggggcaa ggcgatcgac
    25621 ttcaccgccc tgggcaacgc ggcctggttc ggcgcaccga acctgaccgc accatcgttc
    25681 gagccgagcg ccctgaccct gatcgcgccg gtcgcgatca tcctcgtcgc ggagaacctc
    25741 ggccacgtca aggcggtggc ggcgatgacc ggccgcaacc tcgatccgta cctcgggcgg
    25801 gcgttcctcg gtgacggcgt cgcgacgatg atcgccggcg ccggcggcgg gacgggcgtg
    25861 acgacctacg ccgagaacat cggcgtgatg gccgtcacga agatctactc gactctcgtg
    25921 ttcgtgatcg cgggcgtcgt cgcgatcgcg atgggcttct ctccgaagtt cggggcgctg
    25981 atcgggacga tcccggcgcc ggttctcggc ggcctgtcca tcgtcctgtt cgggatgatc
    26041 gcggccaccg gcgcccggat ctggatccag aacaaggtcg acttctcgaa caatcgcaac
    26101 ctgatcacgg ccgcggtcgc gctcacgctc ggcgcgggca acctcgcgat cacgatcggc
    26161 gacttccagc tcggggggat cgggaccgcg acgttcggcg cgatcatcgt ctaccagctg
    26221 ctgcgcgagc cgcggtcgac cgtctcggtc gagacgaccc ccttggtcga gccgaccggc
    26281 gccgcgacgc cggcctgacg cccgccaccg gacgaagccg cgacccgcgc gcgcgaggag
    26341 cgctgcgggt cgcgggtcgc gcctcgggcg cggcgcatgg cccgtgacat tccgcccgtc
    26401 catggcggga cctttggcac gtgtcccgcc ccgcctgccg gcgcctcctt gactgagcgt
    26461 cccatgacca gcggtggccg ccggcaagac ggatggcgca ccgctcgaga tcagcctcgg
    26521 atggggtcga gccatggcgc gtcaagacgg tctctatgcc gcgctactgg agcgcggggt
    26581 cacgcgacgt gacttcctga agttctcggc ggcgatgagc gccgccctcg ccctcccggc
    26641 cgggtacgcg ccgcggatcg cggcggccgt gacgtcggcg ccgagggtcc cggtgatctg
    26701 gctggagggg caggactgcg ccgggaacac cgaggcgttc ctccgggcct cccatccgac
    26761 cgtcgccgag ctggtcctcg acacgctctc ggtcgactac cacgagacga tcatggcggc
    26821 ggcgggccag caggccgagg cggcccggct ggccacgatg aaggcgttcc cgcacggcta
    26881 catcgcggtt gtcgagggat cggtgcccgt ggccgacggc ggggtgtact gcacgatcgg
    26941 cggcaaggcc ttcagcgcca ccgtgcgcga ggtgtgcgat ggagccctcg cgacgatcgc
    27001 ggtgggctcg tgcgcgttcg acggcgggct gccggccgcg gccggcggga cgaccggcgc
    27061 gatcggcgcc ggccaggcgg tccccggcgc gaagatcatc aacctgccgg gctgcccgat
    27121 gaatgtccag aacctgacgg cgacgatcgt ccacttcctg accttcaagc agtggccggc
    27181 gacggacgtc ctcgatcggc cgctcttcgc ctacggccag ctgatccacg accagtgcga
    27241 gcggcgggcc cacttcgagc ggggcgaata cgtccaggcc tggggagacg atgcatcccg
    27301 caagggctgg tgcctttacc agatgggctg caagggcccc gagacgttcg cgaactgccc
    27361 gaccgcgcgc ttcaacgacg gcacgagctg gccggtcaaa gccggccacg gctgcgtcgg
    27421 ctgcacgatg cccaacttct gggaccagat gagcccgttc taccggcgcc tgccgacggt
    27481 cgggccgttc gcgaccgacg tcacggcgga ccaggtcggg ctcgggctgg tcggcgtcgt
    27541 cacggccctg accgcagtgc acgcggcggg aagcgccgtc cggaagcgac ggaccgaggc
    27601 ccgggtccgg gccgagctgg ctgccgtcag cgccccggtg acggcgggga cgacccctca
    27661 gtcgacgccg cccgatgagc agacagagaa ggacggacag gcatgacccg catcgcggtc
    27721 gatccgatca cccggatcga gggacacctc cggatcgagg tcgaggtcgc caacggcgtc
    27781 gtcaccgacg cctggagctc cgggacgatg ttccgggggc tggagctgat cctcaagggc
    27841 cgcgacccgc gcgaggcgtg ggtcttcgcc cagcgcgcct gcggcgtgtg cacgaccgtc
    27901 cacgccctgg cgtcggtccg gtcggtcgag aacgccctcg gcctgcggat cccgcggaat
    27961 gcccggctcg tccgcaacct gatcgccgga gcccagtacg tccaggacca cgtcatccac
    28021 ttctaccacc tccacgccct cgactgggtc gacgtcgtct ccgcgctgaa ggcggacccg
    28081 gatgcgacat cccgtctcgc ccagtcgctg agcgaccatc cgctctcgag cgccgcctac
    28141 ttcaagggcg tccgggaccg gctcaacacc ttcgtccagt ccggccagct cggaccgttc
    28201 gcgaacggct actgggggca tccggcgtac aagctcccgc ccgaggcgaa cctgctggcc
    28261 gtcgcccact acctcgaggc gctcgactgg cagcgccagg tcatccggat ccacgccctg
    28321 ctcgggtcga agaacccgca cccgcagacg tacgccgtcg gcgggatggc cctctccctc
    28381 gaccccaact ccccgaccgc gctcaacgcc cgcgggctgt ccgagatcgc cgaactgatc
    28441 gccgaggcga agcggttcgt cgacgaggtc tatatccccg acgtcctcgc gattgccggc
    28501 ttctacaagg actgggcggc catcggcaag ggcatcggca actacatgtc ctacggcgag
    28561 ttcccggagg acgactcggg tgccgaggcg gcgttctacc tgccgcgcgg ccggatcatg
    28621 ggtcgcgatc tcggcaaggt ggagtccgtc gaccagatcg gggtcaccga gacggtcgac
    28681 cactcctact acacctacga tggcggcgac gcgacgccac tccatccgtg gtccggccag
    28741 acgcacccga agtacgcggg gccgaccccg ccgttcgaga cgctcgaggg ccacgacaag
    28801 tacagctggc tgaaggcgcc gcgctaccag gacgagccga tggaggtcgg gccgctcgcc
    28861 cggatgctcg tcgcctacgg ttccggccag cccgacgtca aggccgcggt cgacggcgtg
    28921 ctcggcaagc tcggcgtcgg accggaggcg ctgttcagca cgctcggccg gatcgccgcc
    28981 cgggcgatcg agacccagct catcgtccac cgcctcgagc gctggctgac cgatctgacg
    29041 gacaacctcg cgacgggcga cctcgcgatc gccgacacga cgaagtggga cccggccacg
    29101 tggccggccg aggcccaggg ctggggcttc gaggaggcgc cccgcggcgc cctcgggcat
    29161 tgggtcagga tcaaggaccg ggcgatcgac aactaccagc tcgtcgtgcc ctcgacctgg
    29221 aacggctcgc cacgcgaccc gaagggacgg cgcggggcgt gggaggaggc cctcgtcggc
    29281 acgcccgtcg ccgatccgaa ccagcccgtc gagatcctgc gcaccgtcca ctccttcgat
    29341 ccgtgcatgg cctgcgcggt ccacgtctac gaccccaagg ccggcagctc cgtccagatc
    29401 cgggtcgtgt gaggcggatg caatgacgac gcctgtggtc acggccgacc cgatcgtgcg
    29461 gacggtcccg gcgccggccc ggccggtcga ggagaaactg cccccgacga tggagcgggt
    29521 ccggatctac gtctggcagg tcccggttcg actcacccac tgggtcacgt tcggcaccct
    29581 gatcgcgctc gcggtcacgg gctcctacat cgcggatccg ttcctgatca cgcccggcca
    29641 ggacacgatg cgtctcgtcc ggttcgtcca catcgtcgcc gcctacagct tcgtcgcgtc
    29701 cgggatcgtc cggacctact ggctgttcgc gggcaaccag ttctcgcgct ggacggcatt
    29761 cgtccccacc aaccggcacc atctccgcga gctggcgagg cagacccagt ggtacctgtt
    29821 cctgcgccgc gacctgcccg gcatcctcgg ccacaacgcg ctcgcgagcg ggacgtacct
    29881 cgtggtcttc ttcctgttcc tgctccagac agcgaccggc ttcgccctcg tcgggatcca
    29941 cggcacccag ccctgggcga cgctcctggg ctggctgccg ggcgtgctgt tcggtgagca
    30001 gggcgtccgg ctgatccacc acctcctcat gtgggcgatc atcgcgtttg cgatccacca
    30061 cgtgtacagc gccctcctcg tcgaccacat cgagcgcaac ggcctcctct cgagcatctt
    30121 cagcggctcg aagttcgtgc cgcgctggcg gatcgtcgag gcccgcgacg gcggcatcga
    30181 gctcgattcg atcgtccgcc gccaggacat cgaggagtcg cgcgccgagg ccctcgacaa
    30241 ggcaatgcga ggcgaggaat gagcggcccg gcgacgccgc tcgtcgtggg catcggcaac
    30301 gtgctcctgc gcgacgaggg cgtcggcgtg gcggtcgtcc gcgagctcga gcggcgcgcg
    30361 gccgagggtc ggctcgacct cccgccgggc acccggttgg tcgacggcgg cacgctcggg
    30421 ctcgatctcc tgccgctcct ctcgagcgcc tccatgctcg tcctcgtgga tgcggtcgac
    30481 ctcggacggc cgcccggaac ggtctgcgtg atccgcgacg cggccatcga gggcactctc
    30541 gccggccacg tgtcccccca ccaggtcggc gtcgccgacc tggtcgcggc tgcccgcctc
    30601 atcggggtct tcccggcgac cgcgtcgctc gtcggcatcc agcccgcgct gatcgagatc
    30661 ggcctcgagc tgagcgacga ggtcgaggcg gccgtcggcg aggccatgga gcgcgtctgc
    30721 gccgagcttc atcggggacc cgacgccgca gcggcggccg cctgatgcac gaggccggtc
    30781 tggccgcgga gatcgccgtc cggctgcagg agggccgccg gaccgggcgc gccgggcggc
    30841 cgcgggtcct ggtccgcggc ggccacgacg cgtccgcgga cttcgacgcg tccctccggt
    30901 tccacctcgc gctcgtggcg ccggggctct cggccgggct cgagatcgtc cacctgcccg
    30961 tcgcccggct gtgcgcctcc tgcggccgcg gcttcgagga cgccgaggtc ttcgcgacgt
    31021 gcccgtcgtg cggcggcgcc gcattgccgg tgtcgaccgg cgaagaggcc gtggtcgaat
    31081 ggtcgccgga aatggcgggc tgatcggatg tgcgtcgcgt tcccgggccg ggtggtcgag
    31141 gtcgacgacg tcggggcggt cgtggacacc gacggccgcc tgcggcgggt gtcgacgctg
    31201 ttcctgcccg atgtcgcggt tggcgactgg gttgcggtca tggccgacac gatcgtggag
    31261 cggctcgagg aggcgcatgc cctcgaggtt cagcagatcc tgcgcgacgc gatcgccctc
    31321 gaggtggccg aggagaacgg gcaaccgcgt gcggacgaga tgccggtccc gggcgccccg
    31381 cgggtgacga caggcaccat ctgaatcgcg ccgcagggcc cgcccgctct gatgacgcct
    31441 gcgtcccggg tggtgatgcc gataccatga tccccccacg atcgcaccca gcgagacgcc
    31501 gatgaccgac ctgccgcaga ccgagcgacc acccttcgtc gccgcgccga cccgctacga
    31561 cgccatgccc taccgccgcg tcggacggag tggcctcgac ctcccggcga tctcgctcgg
    31621 gctctggcag aacttcggcg aaaaccgccc gctggcggtc caacgggcga tcgtccggct
    31681 cgcattcgac cgggggatca cccacttcga cctggccaac aactacggcc cgccgttcgg
    31741 cgccgccgag gagacgttcg gccggatcta cgcctcggac ctgcggccgt accgcgacga
    31801 gctcgtgatc agcacgaagg ccggctacga catgtggtcc gggccgtacg gcgaccgcgg
    31861 ctcgcgcaag tacctgctcg ccagcctcga ccagagcctc aagcggatgg gcctcgagta
    31921 cgtcgacatc ttctattcgc atcgggtcga cccggcgacg ccgatggagg agaccctcgg
    31981 cgcgctcgac acggcggtcc gttcgggcaa ggcgctgtat gccgggatct cgtcgtactc
    32041 ggccgaccgc acgcggaagg ccgccgcgat cctcgccggc ctcgggacgc ccctgctcat
    32101 ccaccagccg tcgtactcga tgctcaaccg ctggatcgag ggcggcctgc tcgacgtcct
    32161 cggcgagacc ggtgtcggct gcatcgcgtt caccccgctc gcccagggcc tcctgacgga
    32221 tcgctacctc gatggcgtcc aggccggctc gcgggtcacg gccaaggatt cgttcgcgcc
    32281 ggagttcctg agcgaggcga acctggccaa ggtccgggcg ctgaacgaaa tcgcgaaggc
    32341 gcgtggccag tccctcgccc agatggcgat cgcctggatc ctgcgcgatc cgcgggtgac
    32401 gtcggcggtc gtcggggcga gctcggtcga gcagctgacc gacaccctcg gcgcactcga
    32461 tcagctcgac ttcagccccg acgagctggc cgcgatcgat cgccacgcga ccgagagcgg
    32521 cgtcaacctc tgggcggcgt cgagcgacgc ctgacgcgtc ggccgcgaga actgcaaccc
    32581 atctgcgatc gaacgcccgg gcatcctggg gcgcacgact ccaccgacgg cgcacgatcg
    32641 accgcgcgtg ccgcgaacgc gggacggacc ggcgagcctg acccggctgg aggatcgatg
    32701 cgatgtgcct gacctgcgga tgcatggacg cccacctcga gatgggcgag tcgaacatca
    32761 cctacgagga cgtgaagcgg gccgccgacg agaacggccg gtcggtcgcc gagacgctcg
    32821 acatcatgga gcgcaccaag gcaaaggatc gcgacgacca tccccaggaa tacgcgacgc
    32881 cgaacgcggg atcctagtcc gcggcgcccg ttccggcgga cgccctgacg atccgccgcc
    32941 agacccgctc gccgtcgacg agctcgtcgg tcggggcgag cccggcccgc tcggccacgg
    33001 cggccgatcc gaggtggtcg gggtggacgt gggcgaggat cgaccgcacg ccggcggcct
    33061 ccagccacgc caccagcccg cgggcggcct cgctggcata gccacggccc tgccacggaa
    33121 cgccgatcag ccaggcgatc tcggcacccc gcccggcgcc ggcaccgccc acatcgacca
    33181 cggtggcctg gacgaagccc gtggccgcgc ccgacggacg ttcccggacg acccagttgt
    33241 gccattcctc gcgtccgtcc ggcgaccggc cacggacctg tgcctcaaag cgcgcgacca
    33301 actcgtcgtg ggtcggcggc ccgccgccga tgaaggcgta caggccggga tcggcgagca
    33361 cggcgaccat ctcgtccgcg tcggcgatcc gcagcggcac gagctcgagg cgcccggtgg
    33421 cgatcgtccc ggaggggatg ggcatcggcc gggcaaggct acccgagccc ggccgatccg
    33481 cggttcggtc agacggtggg gaccgcctcg accgccagcg gcgccccgcc cgacatccgg
    33541 gcgacggcct tctcgagctc ggcgatctcc tcctccggaa ggtccgactg cacggcaaag
    33601 ccgccgaaca gggcgagacc gacgatcgtc aggtgcggcg cgtccgccgc cagatcggcc
    33661 gccgtgcgaa cgtcctggag cccgccgatg ccgcggacgc gggcgctgat gcgccacgac
    33721 tcgggcacga cgatctggcc gccgccgaac accgcccgga cgcgaaggac ggccccgtcc
    33781 ggatggaggg tcgcctcgcg caggtcgacg aacccgccgc cgtaccagca gtcgatgacg
    33841 ccgccgcgga atgcggcggc gcggctcgtg aaggccatcg gaccgagcgc cgtccgcacg
    33901 tggatctcgt ccgcctcagg ctggagcgcc gccatcgtct tgcgggcggt ggaacgcgac
    33961 gcgatcgcca cggccagggt cgagagcatc agcgccgccc agaccatccc cgccgtccgg
    34021 agggcgacgg tcagcccgtg cggcatgcgc cgcttcgggc gagtttccgt ccggtcgcgg
    34081 gtcatggcaa gcaaacgctc ccggaaggtc gctccacggc tctccgccat ggtcccgagg
    34141 tgctcggccg tcgcctggag ccgctccggc gacagcccga gcccggcgat ggccgatcgg
    34201 atccgctcga tctcgaaccc cttggcgtca acgtgttcca cggatgtcat gacgaacctc
    34261 cagtcctcgc cggcgcgggc gcccggaacg gcggcctcgg gtatgtcgag gacgtcgtcc
    34321 ggcgcatttc gacgctgcgg ctggcaggtc gcgcggccgg gctgcgaggg cgatcgtcgg
    34381 tcaacccggt cgcggatacc agtgccgtcg gacccggaac ggcggagggg tgcgcctgcg
    34441 gtgccacggc cgggtccctc cgatccggtc gccggcccgg aagccagcgg acccggcggg
    34501 tcggcctacg gtgacgcgtc ggccgcgccc gccgggcggc gggtgacgaa ccgccgccaa
    34561 cccagaacca gccggtcgtt cacccgggcg acgattcgcg tccggtggat ccgcacgacc
    34621 gcgccagtcc aggcgagcgc gatcagcccc agcgacaggt ggatcccgat cgggagggcc
    34681 gagagctgga agatgtcgcg ccccgcgtcg gtcgccagga cggcgaggta ggccacgacc
    34741 agcccaccga ccagcagcgc cgtccggacc cgggccggca tccggatccg gccgctgtcg
    34801 tccgccgcga tcgggatgag cacgagggcg ccgatgccga ggaacgtcgc catcgtggtc
    34861 agcatcgtcc gtgcgacatc ggtcggggcg ccgatggcga tggcgtcggc atagacgggc
    34921 aggccgacgg cggcgacgcc gagggacagc gggatcgaga tgcggagcgt ctggccgagg
    34981 aggccggacc gcgacggaac cgggcgaacc cagagggcga ggacgaggat cggcacgccg
    35041 accgtgacca gcgcgaggat cgagttctgg cggggcgtga acgggaacgg gagctgcagg
    35101 agcgcggcgc cgacgatgat cagcaggacg tagaacgtcc gggcgaggag gacggtcagg
    35161 gtcgccgcca tcgccgccac gatccgctgg ccgtcgcgga tggcttcggg caggatcgag
    35221 aagtcgtcgc ggacgaggac gaggtcggcc acgccacggg tcgccgggct gccgctctgc
    35281 atcgccacgc cgacgtcggc gatccggacc ggcaggatgt cgttcacgcc gtcgccggtc
    35341 atggccacga accgcccgcg tcgcttgaga accgtcacga ggcgggcctt caggtgcgga
    35401 tcgacgcgcc cgaagatcgt ggtccgaatc gcgaggtccg ccagctcgtc atcgccgagg
    35461 tcggcgagat cgagaccgga cgccgcgttc gtcccgcctc cgagcccgag ccgccgcccg
    35521 atcgcctgga ccgtgaccgg atcgtcgccg gacacgatct tcacgtcgat cccacgagcc
    35581 cggaggccgt cgatcgtggc cggggcctcg gggcggagct cctcctcgaa cgtcagcagg
    35641 gcgatcggtt cgaggcgatc ggggaggcgc ggctggtcgt cgaccgcgag ggcggcgccg
    35701 gccgatgcac gggcgaggac gaggacgcgc tggcccgtct ccgcgctcgc ggtggcccgc
    35761 gaccggacct cgtccgcacg atcgcgcatc gcgggcgcca ggaccgacgg ggcgccgagc
    35821 agccatgtcc cggccgatgc ggggtggtga gcgtcgcccg cggcgaatcg gatcgcggac
    35881 cagcgcagcg ccgacgagaa cggaatctcc tcggcgaccg ccaggcccgg accggggaac
    35941 gcggtcgcga gggcataggt cgtccggttc ggtgtcgtcc cgctcgccgc caccgcggcg
    36001 acgacccgct cgagatcggc cgtgctcatg ccggcggtga ccagccgacg aaggtcgcgg
    36061 aacgtgatct gctgtgtcgt cagggttccg gtcttgtcgg tgcagaaggt gtcgatcctg
    36121 ctgatcgcct cgatcgcgct cgggcgctgg gtcagcgccc ccaggcggga gatccggagg
    36181 gccgccgcgg cgtacgtcac ggtcagcatc agggcgagtc cctggggaac gagcgtgatc
    36241 agcaccgcgg cggcgaacac ggtctcctgc gccgcgtccg tgatcgccgt cgggagcgtc
    36301 aggacgacga cgcccgcggc aagggcgatc agggccccga cgacgatgaa cgtccgggca
    36361 atctccgact ggagcggcgt gcggacgacg cggcggccct tggcctgggc gaggatccgg
    36421 ttggcatacg tgtcggcgcc gaccgcctcg acctcgtagc gcgcgacccc gctgaggacg
    36481 gcgctgccgg actggacggt gtcgcccgcc gacttgtgga tcgggttcga ctcgccggtc
    36541 agggcggatt cgtcgaccga cgccgtcgcg tcgccgacga tccggccgtc gagctggatc
    36601 tcgtcgccgc ggttggcgac gacgacgtcg ccgagaacga ccgcggcggg cgggacctgg
    36661 cgctcgacgc cgtcgcgaac gaccgtcgcc ggcgcctcgc tcaggagcgc gacgcggtcg
    36721 agctggcgct tggctcgcga tccctggacg accccgatcg cgatgttgag gaagaccggc
    36781 agtccggtca tcacggcctc ggtcgtggcg ccgaagccga tcagggccag ggacaccagg
    36841 aggagcggcc cgttgatgaa cggatagctg ttgtcacgga cgatctcgag gtacgaccgg
    36901 ctcgtcgggg tgtggaattc gttggcctcg ccggcgagcg tccgggcctc ggcctccgcc
    36961 gtcgacaggc cgcgctgcgg atccgcggcg ggacgcgcgc cgacgcgcgg tggaacggca
    37021 cggctcgacg tcatggaccc atcgtcgccg ttgcggccgc ggccggcatc gggccgttcg
    37081 gccccgtcgg acgcggaggc gccccgcgag cgagcgagtc gccccggggc gccgatgacg
    37141 gtgtggtcgg ccgggcgatc ccgtccggcc gggagtcagc tcctgaggaa cgccgccatc
    37201 gcggccccga ggaccgtcgc gtcgacgtcg tggcgctggc cctcgaggac cgcggtccgg
    37261 ccgtccggca acgcggcgac gagcgcctcg gcggtcggtc ggaagaacgg gaagctcgcc
    37321 ccgccggtca ggatcagggt cgggacggtg acccgggccg cggtcgcggt cgggaccgag
    37381 tagtcgcgca tgacccgggc gtcgtaggcg agggtgtggg cgatcgcttc ctgggcggcc
    37441 caccacggct gggtcttcgc gaaggcggcg aagtcggccg gcaggcggac catctcgacc
    37501 atgaagaacg tggccgcgtc accgcgccgg ccctcggcga ccagccgatc gagctgttcg
    37561 acggaatcgg ccggcggccg ccccgccgga tcgaggttgt agggcggctc ccacaggacg
    37621 agcttgtcga ccggggcgcc ggcggctgcg gcctcgaacg cgagcccggc gcccgacgac
    37681 gagccgtaca ggtgagcctt gccaccggcc agggcgatga cggcctcgat gtcctcgatc
    37741 tcgcgctcga cggcgtaggg agtcgtgtcg ccgctgtcgc cgcggccgcg ccggtcgtag
    37801 ttgtaggtcg tgaagtccgc cttcagggcg tcggcgagac cggcgttcga gccgcggtcg
    37861 accgagccgc cactgacgag gacgatcgcg gggccgctgc cctggacgtc gacggcgagc
    37921 ttcgtgccgt ctttcgagat gaccgtttcc atcgggatgc tcctttgctc gtggcgggtg
    37981 tcgggtagtg ggtgggcgct caggcgaagg tgcggtcgac ccaggcttgc caggctgcct
    38041 cggtctgctc gcggtcgacg ggctggaaga tgtggtggcc gagcgcggcc gtccccatcc
    38101 catggatgaa gcggtacatc ccgtccgccg tccggaggcc gatgaagtcg ggcgacacgt
    38161 agtcgatctc gccggcgagg tccggcaggc cctcggcccg gaagtgcgcc ggctgcccga
    38221 cgaccggctc gcgggtcacg ccgagcgcgt ccgtgaaggc cgcccatgcc cgctggcgcg
    38281 agacctgcgg accccagagg gtgaccggca ccgcgatccg gccgcggaag tactcgatgt
    38341 actcaacgag cttcgcgacg taggccgggt cgccgatctt cagcgcgtcg tactcctgct
    38401 cccagtcgtc accgggcagg aagccgctgt gaacgaaccg cagcgtggtg gtgccaccgg
    38461 cgcgcccctc gatctcgaag gcgaacgtca tgagccggcc gtcgtcggcc tccgggctcg
    38521 tgttggcgaa tcggtgcggc gggtcccacg tcgtgaccgt cgagtccatc gtgaacccgt
    38581 gcagcgtcgt ccggaccgcg ccgccgagcc gcggctcgac gtcgttctcg cccatgaacc
    38641 aggcgtcgag gccggcagcg gtggagaccg cctcccagac ctgctccggc gtggcgttga
    38701 cggtccgctc gacacggact tcgaacgggt gggccatctc aggattcctc ctcgatctcg
    38761 ggagtcactg gcgcgccgag gtcggtcgcg gccgggacgc tggggtggat ggcgacgacg
    38821 atgcggtggg gtcggccgcc ggccgccgat tcgtcgtgat acctggcgac gagggccgcc
    38881 acggcatgcg acaactcctc ggcgaacgcc gaccgatcgg cggccgaggc gaaccggatc
    38941 tcgccgtcga gggcgaaggt cgcgatgcgc ttaccggcct tgcgggcgcc gatgaggagc
    39001 gacccgacgt cgcggacgag ccgcgccgcc agcgcgagca tccagaacgc cgacaggcga
    39061 tccggggatc gctccgggtc cggctggacc gcggcgaggg ccgcgggcga gatgacgtac
    39121 gaggccgcgg tcgcgcgcat cagccgctcg gtcatgttgc ccttgcgacg ttcctcgacg
    39181 agctcgacga ggccatggcg ctcgagggtc cggaggtggt agttgacctg ctggcgcggc
    39241 aggccgacct tgagcgccag ggtcgtggcc gacccgggtg tcgacagctc ggcgagaagc
    39301 cgcgaccgga ccggatccaa cgaagccgcc gccgccgccg cgttctcgat gaccgtcacg
    39361 tcaagcacgg cgtcatcctg tcaccgacaa attcatttgt caatacagga attatcgtca
    39421 aggcggtgct acgcgccgcc acgcgccagg aacgtcaccc aatctccgtc ggggatcacc
    39481 tcgcccatgt ccgcaggcgg cccgttgtag aggtagaccc aggccgcggc aaccgggccg
    39541 tcgacgatgc ccaccacacg ccggacgtac tgcgaacccg cctcgtcggc aggatcgaac
    39601 gcctcgaggt cgtcggcagc ggcgagcgcc gccgggtcgg ggatgtcgta cagctccacg
    39661 acgacccggc tcggaccgcc gaggacgagc gccggatagg cgtaggcgcg ggcagccgag
    39721 cgcggcatct cgaacaggcg accgaggacc gagccgcgcc cgaggaaggt cgaacccgcg
    39781 aggaaggcgt ggtttcgctc gccgcgccgg agcgtcccgt agacggcgag gacgatgggc
    39841 gtcggggtca tccgagcgag tgtggcacgt cgccgtgagc gcgggccgaa tcgtcacggg
    39901 aacgagcccg gtcgatctcc gggcccttgg caagccggcg gccggaccgc atgctgtccg
    39961 cgccgggagg gcgcgagcca cgccggaggg acggctcgtc ggaggtgtcg acatcgatcc
    40021 gatgctcttg tacgcggtca tcacgatcgc cttcctcgtc ctcgggatcc tcgctctcgg
    40081 ctacggcgtg gacagtcgcg acgggttcga ggacgacgac tggcggcggc gcagctgagc
    40141 acgatcgaac cggctcccga actggatcga cccgatcggc ggcctcagcc gatcgcgccg
    40201 cgggcgacaa tggcgtgacc acacaggagt gacggatgac cgactggaat gcctttgctg
    40261 ccggcgcccc gtccatcgcg gccgccggac gccgcttgat cgagcgatcc gggatcggcg
    40321 agggcctcct cgccacggtc cgcggcgacg ccccgccgcg gatccacccg gtccacgtcc
    40381 ggatcgtcga cgggcggctg ctcacgttcg tcgtcgtggg atctgcgaag agcgcggatc
    40441 tggaggcgga cggccgctat gccctgcacg cccaccagga cccggccgtc ccccacgagt
    40501 tcctcgtccg cgggctggcg accgaggtgc gcgacgcgga tctgcgggcg accgctgccg
    40561 gagaatggtc gttcgaggtc gacgacggct accggctgta cgaactgtcg atcgatcacg
    40621 ccgtcttcgg cgagcgcgcc accgccgacg actggccgcc ggtctacacc gcatggcggg
    40681 ccggcccatc cgcttgagcg acgggacgca ccatcgcgcc gacgcgatgc ggtcgggtcg
    40741 cccgatcgag gttacgctgc agggcacgtg atcgacccca gctccctccg gcgacgagcg
    40801 ccgagcatcg atcgttcgtc ggaccggctg gtcgccgtga ttgcgattgg cgcggccgtc
    40861 tcgttcctca tcgccgctgt cctggcgatg ttccttccgg tcgtcgatcg gcgaggcgcc
    40921 tggctcccca tccacctcgc cctcgccggc ggcgcaacga ccgcgatcgc cggcgtcatg
    40981 ccgttctttg cagcggcctt cgccgccgcg ccaccgagcg acgcccggct gcggatgagt
    41041 gcggtcctgg ccgtcgcggc cggcgcgatc ggtgtctcag tcggcgtcgt cgggtcgcag
    41101 ccggatgtcg cggtccgggg tggcgtcgcg ttcatcgccg gagtcgtcgt gacgggcgtc
    41161 gccacgatcc ggcccctggc gcgcgccctc ggcccgagtc gcggcctcgt gagccagggc
    41221 tatgtcgttg ccctcgccga ggtcgcggtc ggggcgatcc tcgcgacgct gtacgtcggc
    41281 ggctgggcgc cgatgctcgc ggcatggggc caggtcaaac ccgcccatgc atggctcaac
    41341 ctcgtcggat tcgtgtcgct ggtcgtcgcg accacgctgc ttcatttctt tcccacggtc
    41401 gtcggggcgc ggatcacggt ccggccgagc gcccggctca cggtcgtggg gctggcggtc
    41461 ggcgcaccag tggtggcgct cgggttcgcc atcggttccg atctgctcgc ccggatcgga
    41521 gcgctcgcca gcgccgcggg ggcgatcggc ctcgcggtca acgccgcccg gacctggcag
    41581 acacgcgctc gctggacgac cgatccgggc tggcaccggt tcgcgatggg agcgctggtc
    41641 gcggccatca cgtggttcgt cgtcgggatc gcgatcgcgg tcgggcgggt actcgtggcg
    41701 ggcgcgtcgc cggcggcgtt ctcgatcgag acggtgggcg cgccactcgt cgcgggctgg
    41761 atgggcctcg cgatcctcgg ttcggcgacc cacctcctgc cggccgtgc
//
LOCUS       DATAIW010000253        14512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_244761, whole genome shotgun sequence.
ACCESSION   DATAIW010000253 DATAIW010000000
VERSION     DATAIW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244761"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VHM48_08015"
     CDS             <1..773
                     /locus_tag="VHM48_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2755392.1"
                     /translation="LGLAAAAAITLIVTGGAIAPIRRSFAAQRRFVAFASHELRTPAA
                     LIRANADVIEREGLALPAGRPLVADIIAESDRLAHLVGDLLELASIEASGLAIDARPV
                     DLAELASATAREAQPLAAGRGVHVVARADPSSVTIVGGDRNRLVQLLLILLDNAFDHS
                     PAGGTVTIAVRRADRNVELAVSDSGPGIPASDRERVFEPFTGLPGGRRDRAGGTGLGL
                     SIARRIVSAHRGTIRVDDTRAGGGATLLVTIPALTAAP"
     gene            965..1255
                     /locus_tag="VHM48_08020"
     CDS             965..1255
                     /locus_tag="VHM48_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2755393.1"
                     /translation="MAFVIAQPCIDHADQSCVAVCPVDCITADPSVDRKFYIDPDACI
                     DCGSCESACPNGAIFALDRLPAEWADFAWVDAAWYRDPDAARAVVDEFEPAA"
     gene            1323..2066
                     /locus_tag="VHM48_08025"
     CDS             1323..2066
                     /locus_tag="VHM48_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2755394.1"
                     /translation="MAGPPTLAANARIVEREDLTDSIARFVVRPDGELARVEPGQYLT
                     LGLVIDGRLLQRPYSTATAPGERDALEFLVRRVPGGAFTPHLWKLRAGDRLRIGRPKG
                     MFTRIPGDLRTHLLVATGTGLAPFKAMVEASGREPVRPRTVLVHGVSRLAELAWRDRF
                     ERLAAANELDYVPTISRPADPANDGWTGRTGRAESVLDAVWTTAGLEASGTVAYLCGN
                     PSMIAAAREILLGRALADDAIRSEHYWPG"
     gene            complement(2125..2625)
                     /locus_tag="VHM48_08030"
     CDS             complement(2125..2625)
                     /locus_tag="VHM48_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2755395.1"
                     /translation="MRTLTSRDPLRNELLRLLDGLDAHMSFEEAVRGFPDEAMNLRPP
                     SVDYTPWHLVEHLRLTQADILDYLTNSDYTGLEWPREYWPDPTASATRTMWDASVAGF
                     LADRAAIGALLADDGRDLHAVLPGTPGHTLLREVRIVADHNAYHVGEFAILRQVMGTW
                     PATEAG"
     gene            2742..4157
                     /locus_tag="VHM48_08035"
     CDS             2742..4157
                     /locus_tag="VHM48_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2755396.1"
                     /translation="MIPDLGLLVIDPGELVAARSQMAFTLGFHIILACLGVGLPSLVL
                     VANAIGLRRRDPIALLLARRWSLVMAVTFAVGAVTGTVLSFEMGLLWPGLMGRFGGAF
                     GIPFAIEGLFFFLEAIFIAIYIYGWERLSPRAHLISGIPVALSGLGGTFSVIAANSWM
                     NQPAGFAIGPDGRVTAVDALAVIFNRATWYEVPHMLLAAYLVTGFLFASVYAVGLMRG
                     SRDRYVRLGFAIPFVAAAIAAPVQVVVGDVAARAIFEDQPAKFAAMELITKSGPNQPV
                     TIGGVLVGDEVVGGLSIPNLGSLLAGFSPDTPITGFDAIPIDQRPPATIVHLAWDAMV
                     GIGTALVGLAVWGLILRLRRRDHATARWFLRATALAGVGAVVALEAGWMVTEVGRQPW
                     VVYLVLRTADAVSQSGGLRITFAAVIGLYSVLGVATLVILREMTRRWAAADVAAGGRA
                     TDARDASRDEAHVPYGPRGER"
     gene            4154..5197
                     /locus_tag="VHM48_08040"
     CDS             4154..5197
                     /locus_tag="VHM48_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2755397.1"
                     /translation="MTLVDVTAVVLWLGVTTYAVFGGADFGAGFWDLVSGRGDQGERS
                     RGLIATAIGPVWEANHTWLIFDLVILWTAFPVAFAAIMSSLFVPLSLAAVGIVLRGAA
                     FAFRPVVGSAAGRRVTTAIFAASSVMTPFFLGAAGGGIASGRVTLDPTGGDLVGSWLN
                     PTSVLVGLMAVAACAYLAAVFLVADARRLGQADLAALFLRRARVAAVVTGGLAVVGIL
                     VLAADAPVLADELIRRGWPLVIASSLLGGAALVAIGQRAPFGTRPLAVGAVVAIIWGW
                     GVAQYPDILPRTLSLADAAAPSGSLGSLLVVFVAAALLIAPSLVLLFWLAQDNRLPGH
                     VPEAVADPAARVD"
     gene            complement(5203..5946)
                     /locus_tag="VHM48_08045"
     CDS             complement(5203..5946)
                     /locus_tag="VHM48_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2755398.1"
                     /translation="MSSHRTAMVDEIASVHPDHRSSPLVAASDRRPAARSAAGRRAGP
                     HELPYGPFLQRGLPAISRAFRILNRFYVLPAIKLGLGPLHANPLTGSWMLLRTTGRRT
                     GLAREVALGYALLDGNVYCSAGFGARTGWYQNLLANPRVEFILPSGGFAGVAEPVTDP
                     AELDRGWRALIRALGVLGLGFVCSADASPETLAAKTTNLPLIRIRTVGLAGGPADPGG
                     RLWVVVTVVGLVWLAGRIRRNVRERDGIG"
     gene            complement(6112..6657)
                     /locus_tag="VHM48_08050"
     CDS             complement(6112..6657)
                     /locus_tag="VHM48_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX2755399.1"
                     /translation="MAQPSIVREDRWHPGEEAWAMEDEISITEIEEQRIVSIRGRFEQ
                     RTIPEFLGKAFGEVFGRLGLLGVSPAGPPFVIYHQFGPQEVDAEVCVPVSGSVEACGR
                     IRARIIPAMTVARTLHVGPYEELGGAYIALDAWIELGGLEAVGPLQERYLNRPGDEVM
                     PADYQTEIEIPILAATAAVLV"
     gene            6954..8102
                     /gene="asnA"
                     /locus_tag="VHM48_08055"
     CDS             6954..8102
                     /gene="asnA"
                     /locus_tag="VHM48_08055"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEX2755400.1"
                     /translation="MATTTTLTDKEADLAGPGIGDYAELAKVLPTDYRSLLTPRETQA
                     AIFAVKGYIEQNLCRELDLMMVQVPLIVDAESGVNDMLDRDGSRTPVSFHISNDRERH
                     PIDAQVVQAATKWKRIALRQFGLAPGEGICTDMRAVRKDYFLDHDHSAYVDQWDWERV
                     ITADQRDLETLEATVRTIWRVLRGAEQDVQGLFPQLRTERYPDLPEELTFIHAEDLLE
                     RFPDLPRKQRETLILQEYPAVFIIGIGWRLADGYPHEMRAADYDDWATETTAPDGHLY
                     HGLNGDILVWNPVTRRRHELTSMGIRVDAASLRRQLEITGQLDWLELPYHRGIASGEL
                     PLSIGGGIGQSRTQMLLLKKAHLGEVSVTVWPEILKEMCRERHIDVLE"
     gene            8166..8483
                     /locus_tag="VHM48_08060"
     CDS             8166..8483
                     /locus_tag="VHM48_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755401.1"
                     /translation="MPLLFLFACALAGVTTLVAVVTLSRGVSGGVIAGGLLAMYLLDA
                     VSRIDAGLDWLGAFSAFRYLRSTSAIDRGMAPVGEVALFGAIALATWTVAILAFRRRD
                     LVA"
     gene            complement(8574..9056)
                     /locus_tag="VHM48_08065"
     CDS             complement(8574..9056)
                     /locus_tag="VHM48_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755402.1"
                     /translation="MIHPTPTPSQAVRPTPTPFACTALPYNRSSGAVAVDVRDVRVGS
                     HPGYDRIVFETAGTARPEVRIEVVKPPFKLDPSDLPVTIAGSSFIQIKLTGVAVETVP
                     SAARDMVVNAPVLLELRQTAGYEGDATWIAGLSGRACSRISLLSGPTRLVVDVARTGS
                     "
     gene            9429..9851
                     /locus_tag="VHM48_08070"
     CDS             9429..9851
                     /locus_tag="VHM48_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755403.1"
                     /translation="MPSIRFPIRVGRRSRLPLRLLFGVRADDSWVDLGDGPDGALEVR
                     FGWSHFRTQMANVLSWQIEGPFRWITAIGVRKSIRHGDVTFGGSPHGGVRIDFRTPVH
                     WTIFNVPAIYLPADDLEALAAELERRGIPGHDARSRVS"
     gene            complement(9973..11229)
                     /locus_tag="VHM48_08075"
     CDS             complement(9973..11229)
                     /locus_tag="VHM48_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2755404.1"
                     /translation="MDRRPRIPWIGRPSARRAPGPVGRVVIALVAAASVVGAVVLVGA
                     PATGGPGRLASATPSAADPTSAPLLAGHEVYGFVPYWEIDDTIAAHLRATDATTIALF
                     SVTNTSKGAIATTLKGYRAIDGPIGREIVTDAHRLGRRVDMTWTSFGRPRNDALFRSE
                     DLQNRVIASLVALRADLDVDGIAVDVEEIGDTDIPAYGGFVGRLRTALRASDPTATVT
                     ATTGAGPRGAALAFAASLAGADRIFLMGYDYRTVSSEPGASAPISRGDGVDRSIVWSL
                     DLYAAAGVPAGRTLLGLPLYGLAWPTATPEPGSPKTGRGSVWVPRRNLATLLNPAASA
                     SYDPVEVVAFLAVPDGTTWQAIYYDSPRSLRPKLGLANDLGLAGAGFWALGYERGLPD
                     YTALIADFRAGRSMTVGPVTVGPVTP"
     gene            complement(11458..11670)
                     /locus_tag="VHM48_08080"
     CDS             complement(11458..11670)
                     /locus_tag="VHM48_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755405.1"
                     /translation="MKRKRGDAASWTPLHAVTGDDDPLRREEHDGADPAQNDEQRLAH
                     RQVRSRAAIGGEVPTGLPGRGGNAPT"
     gene            12387..13694
                     /locus_tag="VHM48_08085"
     CDS             12387..13694
                     /locus_tag="VHM48_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2755406.1"
                     /translation="MATSTLPRTRNRPSLAKEDLDLAREVGARLKAARTRAGLTQREL
                     AEPRYTKAYISALENGLIKPSMAALRFLARRLGTVPSAFLADEDTHWQRLDAELRLAA
                     GDWRAAADRFQAILDGGPSGMARGLSLLGLAEANYRLGLSRETITCASEALELLTAAN
                     RRADAQRATYWLAAGHIASDDPARARMLFEELLAATAVEDGDPDLRVRILVALATVEV
                     YAGESSAAIGLLEEARAIGADLDDRRRASLLHSLAQSYRMAGDLEGAVRTGIESLALF
                     RAVDARAETASIENELALTYLGLGNLAAAEQHAREARLGMERDPDQFRLAHLGDTDAR
                     IALARGDLELAGRQATAAAELAALLGNQKALVDALLTLARTARRQGDPALAAATLERA
                     ATAAEDGPLARLRLVLGEWSELAADNGDHATAYELSRRALALR"
     gene            complement(13763..14431)
                     /locus_tag="VHM48_08090"
     CDS             complement(13763..14431)
                     /locus_tag="VHM48_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2755407.1"
                     /translation="MIRPTTGARRASSRIAQRARTRRQIVLGLAVAGSLLGSLAAAPG
                     VAAATTTRTAPSSSVALATTTLDKPWFSVERFYLGLVNCTRTGGWVLSDGTCRGYGSG
                     HYSAYVAPIAYSYGLSDKVSRPYAKLLVTRALCSHTADGDPGHRLRRAGFTRWNWGEN
                     IGCRDGYSTAKAAVLASHLKFQAEKSTNGGHWRNLKNPAYHWIGIGIWRSSTRTRLVV
                     DFYG"
BASE COUNT         1993 a         5159 c         5152 g         2208 t
ORIGIN      
        1 cgctcgggct ggccgctgcc gctgcgatca cgctgatcgt gaccggtggc gcgatcgccc
       61 cgatccgccg ctcgttcgcg gcccagcgtc ggttcgttgc cttcgcctcg catgagctgc
      121 ggacgccggc ggcgctcatc cgcgcgaacg ccgacgtgat cgaacgcgaa gggctggcgc
      181 tgcccgccgg ccgcccgctc gtcgccgaca tcatcgccga gtccgatcgg ctcgcccacc
      241 tcgtcggcga cctgctcgag ctcgcgtcga tcgaggcgag cggactggcg atcgacgcac
      301 gacccgtcga cctcgcggag ctggccagcg cgaccgcccg ggaggcgcag cccctcgccg
      361 cagggcgtgg cgttcacgtc gtcgctcggg ccgatccgtc gtccgtgacc atcgtgggcg
      421 gcgatcggaa tcgcctcgtc cagctgctgc tgatccttct cgacaacgcg ttcgaccact
      481 cgccggccgg tgggacggtc accatcgcgg ttcgccgggc cgaccggaat gtcgagctgg
      541 ccgtgtcgga ttccggcccg gggatcccgg cgtcggatcg cgagcgcgtc ttcgagccct
      601 tcaccggcct gcccggtggg cgtcgtgatc gagccggcgg aaccgggctc ggcctgtcga
      661 tcgcgcgccg gatcgtgtcc gcccatcggg ggacgatccg ggtcgacgac acgcgggccg
      721 gcggcggcgc gacgctgctc gtgacgatcc ctgccctgac ggccgcgccg taatacggcc
      781 gcgcggcgag cgccccggat ggggagatcc acatccgggt cacacgcttg ccacatcgac
      841 cggatcgacc gtagggcaca gcggaggttc cggatgaggc gactggcgtc cgacgatcgc
      901 ctgaaacccg gggcctccgc gtcgactcaa cgagtcgtcc gagtcctgct ccaggagcgt
      961 caccatggcc ttcgtcatcg cccagccgtg catcgatcac gcggatcagt cctgcgtggc
     1021 ggtctgcccg gtcgactgca tcaccgccga cccgagcgtc gaccggaagt tctacatcga
     1081 tccggatgcc tgcatcgact gcggctcctg tgaatcggcc tgcccgaacg gagcgatctt
     1141 cgccctggat cgcctgccgg cggagtgggc cgacttcgcc tgggtcgacg cggcgtggta
     1201 tcgcgatccg gacgccgccc gagccgtggt cgacgaattc gagccggcgg cctgacgtgg
     1261 ccatcggacc gacgatcgag cgggcccttc cgccgcgccg cccactcccc ccgaggccgg
     1321 agttggcggg cccgccgacc ctcgccgcca acgcccggat cgtcgagcgt gaggacctga
     1381 cggactcgat cgccaggttc gtcgtccgac cggacggcga gctggcgcgg gtcgagccgg
     1441 gccagtacct gacgctcggg ttggtcatcg acggccggct cctccagcgg ccgtactcga
     1501 ccgcgacggc accgggcgag cgcgacgcgc tcgagttcct cgtccggcgc gtacccggcg
     1561 gggcgttcac cccgcacctg tggaagctgc gcgccgggga tcggctccgg atcgggcgcc
     1621 cgaagggcat gttcacgagg atcccgggtg acctccggac gcacctgctc gtcgcgaccg
     1681 gaaccggcct ggctccgttc aaggcgatgg tcgaggcgag cggccgggag ccggtccggc
     1741 cgcggacggt cctcgtccat ggcgtatcgc ggctggccga gctcgcctgg cgggaccggt
     1801 tcgagcggct ggccgccgcg aacgagctcg actacgtccc gacgatctcc cggccggccg
     1861 acccggccaa cgacggctgg accgggcgga cggggcgggc ggagtcggtc cttgatgcgg
     1921 tctggacgac ggccggcctg gaagcctccg ggacggtcgc ctatctctgt ggcaacccgt
     1981 cgatgatcgc ggcggcccgc gagatcctcc tcggtcgcgc cctggcggat gacgcgatcc
     2041 ggagcgagca ctactggccg ggctgagcct ggccgcggcg cgtccgtccg gacccgatcg
     2101 ttcgcacgct gcggtcgcga gccgtcaacc ggcctcggtg gccggccagg tccccatcac
     2161 ctggcggagg atcgcgaact cgccgacgtg gtaggcgttg tggtcggcga cgatccggac
     2221 ctcgcgcagc aacgtgtggc cgggcgtgcc cggcagcacc gcgtgcaggt cgcggccgtc
     2281 gtcggcgagg agcgctccga tcgccgcgcg gtcggccagg aagccggcga cgctcgcatc
     2341 ccacatcgtc cgggtcgcgc tggcggtcgg atccggccag tactcgcgcg gccactcgag
     2401 cccggtgtag tcggagttcg tcaggtagtc gaggatatcg gcctgggtca gccggaggtg
     2461 ctcgacgagg tgccacgggg tgtagtccac gcttggcggc cggaggttca tcgcttcgtc
     2521 cggaaaccct cggaccgcct cctcgaagga catgtgggcg tcgaggccgt cgagcagccg
     2581 gagcagctcg ttgcgcagcg gatcgcgact cgtgagggtc ctcattcgat cttctccagg
     2641 gtgacggcag catgaccgac ggtcccgacc atgatgccgc agtacgatga acccgtcgtg
     2701 aacgcacctg ttcgactcga ccgaaccgcc cggggacgcg cgtgattccg gacctcgggc
     2761 tcctcgtgat cgaccccggc gagctcgtcg ccgcgcgctc gcagatggcg ttcacgctcg
     2821 gcttccacat catcctcgcc tgtctcgggg ttgggttgcc gagcctcgtc ctcgtcgcca
     2881 acgcgatcgg cctgcgccgt cgcgacccca tcgccctcct cctcgcccgg cgctggtcgc
     2941 tcgtgatggc ggtgaccttc gcggtcggcg ccgtgacggg aacggtgctg agcttcgaga
     3001 tgggcctgct ctggccgggc ctgatgggcc ggttcggcgg cgccttcggg atcccgttcg
     3061 cgatcgaggg cctgttcttc ttcctcgagg cgatcttcat cgcgatctac atctacggct
     3121 gggaacggct gtccccgcgg gcccacctga tcagcgggat cccggtcgcg ctgagcggtc
     3181 tcggcgggac gttctcggtc atcgccgcga acagctggat gaaccagccg gccgggttcg
     3241 ccatcgggcc cgacgggcgg gtcaccgcgg tcgacgcgct ggcggtgatc ttcaaccggg
     3301 cgacctggta cgaggtcccc cacatgctcc tcgcggcata tctcgtgacc ggcttcctgt
     3361 tcgcctcggt ctacgcggtc ggcctgatgc gagggagccg cgaccgttat gtccggctcg
     3421 ggttcgccat cccgttcgtc gcggccgcga tcgccgcgcc tgtccaggtc gtcgtcggcg
     3481 acgtcgcggc gcgggcgatc ttcgaggacc agcccgccaa gttcgcggcg atggagctga
     3541 tcacgaagag cggcccgaac cagccggtga cgatcggcgg cgtcctcgtc ggcgacgagg
     3601 tcgtcggcgg gctctcgatc ccgaacctcg gctcgctcct ggccggtttc agcccggaca
     3661 cgccgatcac cggcttcgat gcgatcccga tcgaccagcg gccaccggcc acgatcgtcc
     3721 acctcgcctg ggacgcgatg gtcggaatcg ggacggccct cgtcggcctt gcggtgtggg
     3781 gcctgatcct gcgcctccgt cgccgcgatc atgcgacggc gcgctggttc ctgcgagcaa
     3841 ccgcgctggc gggcgtcggt gccgtcgtcg cgctcgaggc cggctggatg gtcactgagg
     3901 tcggacgcca gccgtgggtc gtctacctcg tgctccggac cgccgatgcc gtctcccagt
     3961 cgggcgggct ccggatcacg ttcgcggccg tgatcgggct gtacagcgtc ctcggcgtgg
     4021 ccacgctcgt gatcctgcgg gagatgaccc ggcggtgggc cgcggccgat gtcgccgcgg
     4081 gcggccgcgc caccgacgcc cgggatgcgt cgcgggacga ggcacatgtc ccctatggcc
     4141 cgcgcggcga gcgatgaccc tcgtcgacgt caccgccgtc gtcctgtggc tgggggtcac
     4201 gacctacgcg gtcttcggcg gcgccgactt cggggccggc ttctgggacc tcgtttcggg
     4261 tcggggcgac cagggcgaac ggtcgcgcgg cctcatcgcg accgcgatcg ggccggtctg
     4321 ggaagcgaat cacacctggc tgatcttcga tctcgtgatc ctctggacgg cattcccggt
     4381 ggcgttcgcg gcgatcatgt cgagcctgtt cgtgccgctc agcctggcag cggtcgggat
     4441 cgtcctgcgc ggcgccgcgt tcgcgttccg gccggtcgtc ggcagcgccg cgggccggcg
     4501 ggtcacgacg gcgatcttcg ccgcatcgtc ggtcatgacg ccgttcttcc tcggagccgc
     4561 cggcggcggg atcgccagcg ggcgggtgac gctcgacccg accggcggag acctcgtcgg
     4621 gagctggctc aatccgacct cggtcctcgt cgggctgatg gccgtcgccg cctgcgccta
     4681 cctcgccgcg gtgttcctgg ttgccgacgc tcgacgcctc ggccaggccg acctcgcggc
     4741 gctgttcctg cggcgggcgc gcgtcgcggc cgtcgtcacg ggcggcctgg cagtggtcgg
     4801 catcctcgtc ctggcggcgg acgcaccggt cctcgccgat gagctgatcc gtcgcggctg
     4861 gccgctggtc atcgcctcca gcctgctcgg cggcgcggcg ctcgtcgcga tcgggcagcg
     4921 ggcgccgttc gggacgcggc cgctcgcggt cggcgccgtg gtcgcgatca tctggggctg
     4981 gggtgtcgcg cagtaccccg acatcctgcc gcggacgctg tccctggccg acgcggccgc
     5041 gccgtccggc tccctcggct cgctgctcgt cgtcttcgtc gcggcggcgc tcctgatcgc
     5101 tccgtcactc gtccttctgt tctggctggc ccaggacaac cggctcccgg gccacgtgcc
     5161 agaggctgtc gccgacccgg ccgcccgggt cgactgatcg cctcagccga tcccgtcccg
     5221 ttcgcgaacg ttacgccgga tccggccggc caaccagaca aggcccacga cggtcacgac
     5281 gacccagagt cgcccgcctg gatccgccgg accgccggcg aggccgacgg tccggatccg
     5341 gatcaggggc aggttggtgg tcttcgccgc gagcgtttcg ggcgacgcgt cggcgctgca
     5401 gacaaagccc aggccaagga cgccgagggc gcggatcagg gccctccagc cacgatcgag
     5461 ctcggccggg tcggtgaccg gctcggccac gccggcaaac ccgccggacg gcaggatgaa
     5521 ctcgacgcgc ggatttgcca ggaggttctg gtaccagccg gtccgcgcgc cgaacccggc
     5581 cgagcagtac acgttgccgt cgaggagtgc gtagccgagg gcgacctcgc gggcgagccc
     5641 ggtccgtcgc ccggtggtcc ggagcaacat ccacgatccc gtcaacggat tggcatggag
     5701 cggaccgaga ccgagcttga tcgccggcag gacgtagaac cgattcagga tgcggaaggc
     5761 gcgactgatg gccggcagcc cgcgttggag gaacggcccg tacggcagct cgtgcggtcc
     5821 agcgcgccgg ccggcggcgg atcgggcggc gggccggcgg tcgctcgcgg ccaccaacgg
     5881 cgacgatcgg tggtcggggt ggacactggc gatctcatcg accatggcgg tccggtggga
     5941 cgacatggtc acgacgatcg cccatgcggc gccggctgag gagggtgatt cggcccgttg
     6001 tgaccgggcc ggccagccac gtctggacgg tccgcggcag ccggcgcaca gctcatgctt
     6061 cgcgccgacg gccccggtcc cgccgtggcg ggaccgggat cggcaccgtc gtcagacgag
     6121 gaccgcggcg gtcgcggcga ggatcgggat ctcgatctcc gtctggtaat cggctggcat
     6181 gacctcgtct ccgggtcggt tcaggtaccg ctcctggagc gggccgacgg cctcgagacc
     6241 acccagctcg atccacgcgt cgagggcgat gtaggcacct ccgagctcct cgtacggacc
     6301 gacgtggagc gtccgcgcga ccgtcatcgc cggaatgatc cgggcccgga tccgcccgca
     6361 ggcctcgacc gacccggaga cgggcacgca gacctcggcg tcgacctcct gcggcccgaa
     6421 ctggtggtag atcacgaacg gcggtccggc cggcgacaca ccgagcaggc ccaaccggcc
     6481 gaacacctcg ccgaacgcct tgccgaggaa ttccggaatg gttcgctgct cgaatcggcc
     6541 ccgaatcgag acgatccgct gctcctcgat ctcggtgatc gagatctcgt cctccatcgc
     6601 ccaagcctcc tcgccgggat gccagcggtc ctcgcggacg atcgagggtt gcgccacggg
     6661 atggcgatgc ggcccgcccg acacgactcg tccggcgtgg tcgagtctcg ccggtggagc
     6721 ggcgcgccgc cagagccatc ggacccgtcg atgcgcaaac gcgcgttcca ggtctccggc
     6781 ggggctgacc ggccgcagcc gatcgggctg atcggcactc gcggccgacc cgctcgcgcc
     6841 ggacgctggg cctgcgcgat gaccgggttc ggcagcggat cgatccggtc gggacgcgcg
     6901 gttccgtgac gcggcacagg ctcgccgatc cggacgttcg acaaggggac gacatggcca
     6961 cgaccacaac cctgacggac aaggaggccg acctcgccgg ccccggcatc ggcgactacg
     7021 ccgagctcgc gaaggtcctt ccgaccgact accggtcgct gctcacgccg cgcgagacac
     7081 aggccgcgat cttcgcggtg aagggctaca tcgaacagaa cctctgccgc gagctcgacc
     7141 tgatgatggt ccaggttccg ttgatcgtcg atgccgagtc gggcgtcaac gacatgctcg
     7201 accgggatgg ctcgcggacg ccggtctcgt tccacatctc gaacgaccgc gagcgccacc
     7261 cgatcgacgc ccaggtcgtc caggccgcca cgaaatggaa gaggatcgcc ctccgtcagt
     7321 tcgggctggc tccgggtgag gggatctgca ccgacatgcg ggctgtccgc aaggactact
     7381 tcctcgatca cgaccacagc gcctacgtcg accagtggga ctgggagcgc gtgatcaccg
     7441 cggatcagcg ggatctcgag acgctcgagg cgacggtccg cacgatctgg cgcgtcctgc
     7501 gcggtgcgga gcaggacgtc caggggctgt tcccgcagct tcggaccgag cgctacccgg
     7561 acctgcccga ggagctcacc ttcatccacg ccgaggacct cctcgagcgc ttcccggacc
     7621 tgccgcgaaa gcagcgcgag acgctgatcc tccaggagta cccggcggtc ttcatcatcg
     7681 ggatcggctg gcgcctcgcc gacggctatc cgcacgagat gcgggccgcc gactacgacg
     7741 actgggcgac cgagaccaca gcaccggacg gccacctgta ccacggcctc aacggcgaca
     7801 tcctggtctg gaacccggtg acccgccgcc gccacgagct gacctccatg gggatccggg
     7861 tggatgcggc atcgctccgt cgccagctgg agatcaccgg ccagctcgac tggctcgagc
     7921 tgccctatca ccgcgggatc gccagcggcg agctgccgct cagcatcggc ggcgggatcg
     7981 gtcagtcgcg gacccagatg ctcctgctca agaaggccca cctcggcgag gtcagcgtga
     8041 ccgtctggcc ggagatcctc aaggagatgt gccgggagcg gcacatcgac gtcctcgagt
     8101 agccgtcctc gatcgtcggg gccgtcgtcg gcgccggctt cgacgcggtc cgcttcctgg
     8161 tcgaggtgcc gttgctgttc ctgttcgcct gcgccctcgc cggcgtgacg acgctcgtgg
     8221 ccgtcgtcac cctgagtcgc ggcgtcagcg ggggtgtcat cgccggcggg ctgctggcga
     8281 tgtacctgct cgatgcagtg tcccggatcg acgccggcct tgactggctc ggcgcgttca
     8341 gcgccttccg ctacctgcga tcgacctcgg cgatcgaccg gggcatggcg cccgtcggcg
     8401 aggtcgcgtt gttcggggcg atcgcccttg cgacgtggac cgtggcgatc ctggcgttcc
     8461 gccgccgcga cctggtcgcc tgaccgtcca cccgcagctc accgcgggaa cccgttcccg
     8521 gacggggccc ccgcggttgt cacagactgc tgtcgatggg tcgaggtcgt tgctcaactg
     8581 ccggttcgcg cgacatcgac gacgagacgg gtcggtccgc tcaggaggct gatccgactg
     8641 caggcccggc cagagagccc ggcgatccag gtcgcatcgc cctcgtatcc cgccgtctgc
     8701 cggagttcca ggaggaccgg ggcgttgacg accatgtcgc gcgccgccga gggcacggtc
     8761 tcgacggcaa ccccggtgag cttgatctgg atgaacgacg agccggcgat cgtgaccggg
     8821 agatcgctcg ggtcgagctt gaacggcggc ttcacgacct cgatccggac ctccggacgc
     8881 gccgtgcccg cggtttcgaa gacgatccgg tcatagccgg gatggctccc gacccggacg
     8941 tccctgacat cgaccgcgac cgcgccgctg gatcggttgt acggcaatgc cgtgcaggcg
     9001 aatggcgttg gcgtggggcg gacggcctgg cttggcgtcg gggttggatg gatcacggat
     9061 ggggtcgggc tcgacgccga gctcgcgctg gtcgcgggtc ccgagccggc cgacgccacg
     9121 accgatgcac tcggcgaacc gagcgccggg ttggtcgacg ctgatggacc ggcactcggg
     9181 ccgcacgccg tgaagaggag cgccaagagc acggtcccgc gactccaacc tcgtccggct
     9241 cggacgggcc gctcgactgc tggctccgga ttcacgtgtc tcatcactca cctccgccgg
     9301 cccgcgccgg ctcgataccg cgctcagtct gggtccgatc ggccaggagt tcgagagcca
     9361 tccggctcga tgtgccggaa cgcggctcgc gctcggcccg aatcgacgag tcgagctacc
     9421 ctccggccat gccttcgatc cgatttccga tccgcgtcgg gcgccgttcc cgcctgccgc
     9481 tgcgcctgct cttcggcgtt cgagccgacg attcgtgggt cgatctgggc gacggcccgg
     9541 acggcgcgct cgaggtccgg ttcggttggt cccatttccg gacccagatg gcgaacgtgc
     9601 tcagctggca gatcgagggt ccgtttcggt ggatcaccgc gatcggcgtc cggaagagca
     9661 tccgccatgg cgacgtgacc ttcggcggca gcccccacgg cggggtccgg atcgacttcc
     9721 ggacgcccgt ccactggacg atcttcaatg tcccggcgat ctaccttccg gccgacgacc
     9781 tcgaggcgct cgcggcggag ctggaacgtc gcgggatccc ggggcatgac gccaggtcgc
     9841 gcgtcagctg atctcgatcg cctgcccgac gccggacgtc gacggcacga ccaggtgctg
     9901 gcgaacgagc gtcgcccgat tgaggtcggt cgacgtcagg acgctggccg cggccgtggc
     9961 cggctcgggc tgtcagggcg tcaccgggcc gaccgtcacg gggccgaccg tcatcgaccg
    10021 gccggcccgg aagtcggcga tcagggccgt gtaatcgggc aggcctcgct catacccgag
    10081 cgcccagaac ccggccccgg cgaggcccag gtcattggcg aggccgagct tcggtcggag
    10141 gctccgcggc gagtcgtaat agatcgcctg ccacgtcgtg ccgtcgggaa cggccaggaa
    10201 ggcgacgacc tcgaccgggt cgtaggacgc gctggccgcc ggattgagca gggttgcgag
    10261 gttgcggcgc gggacccaga cgctaccgcg accggtcttc ggcgatccgg gctccggcgt
    10321 ggcggtcggc caggcgaggc cgtagagcgg caggccgagg agcgtgcgtc cggccgggac
    10381 gccggcggcc gcgtacaggt cgagcgacca gacgatcgag cgatcgacgc cgtcgccgcg
    10441 cgagatcgga gccgaagcgc cgggctcgct cgacaccgtc cgatagtcgt agcccatgag
    10501 gaagatccga tcggcgccgg cgaggctcgc cgcgaacgcg agggcggcac cacgcggacc
    10561 ggcgcctgtc gtcgccgtca cggtcgcggt cgggtcggaa gcccggagcg cggtccgcag
    10621 tcgaccgacg aatccgccgt acgccgggat gtcggtgtcg ccgatctcct ccacatcgac
    10681 cgcgatcccg tcgacgtcga ggtctgcccg gagagccacg agcgacgcga tcactcgatt
    10741 ctggagatcc tccgagcgaa acagggcatc gttcctgggc cgcccgaagc tcgtccaggt
    10801 catgtcgacc cgtcgcccga gccggtgtgc gtcggtgacg atctctcggc cgatcggacc
    10861 gtcgatcgcg cggtagccct tgagggtcgt cgcgatcgcc cccttggacg tgttggtcac
    10921 cgagaagagg gcgatcgtcg tcgcgtccgt cgcccggagg tgggcggcga tcgtgtcgtc
    10981 gatctcccag tacggcacga agccgtagac ctcgtgaccc gcgagcaagg gagccgaggt
    11041 ggggtccgcg gccgacgggg tcgcgctggc aagccgcccc ggaccacccg tcgctggagc
    11101 gccgacgagg accaccgcgc cgaccacgct cgcggcagcg accagcgcga tgacgacccg
    11161 accgaccgga ccgggcgcgc ggcgggcgct cggtcgaccg atccacggga tgcgaggtcg
    11221 cctgtccacg atcccagtct ggcgcctcga ctcgatccgg tccacgaacg cgatcactcc
    11281 tccgggagtc aaccgcttct tcgccccatc ttcttgaccg gcatggaccg gcgaagtcaa
    11341 gtattctcaa cttgctccag tccggcgttg gggggcgctg gactgcgagc ccgacgagat
    11401 ggagagacac ccttgcctga tcggatcacg cgcatcacgc cacgttcggg cacgggatca
    11461 ggtgggcgca ttgccgcccc gaccgggaag gccggtcggg acctcgccgc cgatcgcggc
    11521 gcgggaccgg acctggcggt gggcgagacg ctgctcgtcg ttctgcgcgg gatcggcgcc
    11581 gtcatgttcc tcacgacgca gcgggtcatc gtcgcccgtg acggcatgga gcggcgtcca
    11641 cgaagcggcg tcaccgcgtt tccgcttcac accattcgcc accttcgcct cgagcttggc
    11701 agcgctccgt cgggccggat cgccgtctgg accgacgcgg gccaagaggc cgtgagcatg
    11761 ttcttcgatg cccgatcact ggatcgagcc catgagctga ttgatgtcgc gaggccgctc
    11821 atcgctcgac agcgacgcgg ccccggagcg cgaccggtcc gtcccccgaa cggaccggtc
    11881 gactccggat cccacgaccc cgcctgactg gacacggttc ccgtcgggac cgccgtcgat
    11941 caccaccatt gatcctcgcg ccgcgggtca cgggcgtttg ttcttcatcg gtgtgatcgc
    12001 atcggcggtc cgtccgggtc ccgtcgtccg ggcgggtttg ccattcctca ggacggactg
    12061 tttcccgcct gatcgttggc gcgggtccgc gcccgccgga tgatcagcgc gggatcggac
    12121 ccgcaccgct ttcggccagc gcggttgcaa cgccggccag gagggtcgct gcggcgagga
    12181 tcgcagagat gacaagggtt cgggctcgat ggaaacgcac ggttggtgct ccttctccgc
    12241 ccgggcccgt ggcgcgagga cgcaccacta tcgtaggcgt tccagccgga ttcacaagta
    12301 tttattgacg caacggatcg ccaccgccgg tcgatcgcgt gcgcggagtc aataaccgct
    12361 tggtgggacg gcgtatgatc cgtgccatgg cgacctcaac gcttccccgg acccgcaatc
    12421 gtccaagcct cgcgaaggag gatctcgatc tggcccgcga ggtcggagcc cgcctcaagg
    12481 ccgcccggac ccgggccggc ctgacccagc gggagctggc cgagccgcgc tacacgaagg
    12541 cgtacatcag cgcgctggag aacggcctga tcaagccgtc gatggcggca ctccgattcc
    12601 tcgcccgccg cctgggcacc gtgccgagcg cattcctcgc ggacgaggac acccactggc
    12661 agcgcctcga cgccgagctc cgtctcgcgg ccggcgattg gcgggccgcg gcggatcgct
    12721 tccaggcgat cctcgacggc ggcccatccg gcatggcccg gggcctgtcc ctgctcggtc
    12781 tcgccgaggc aaactaccga ctcggtctct cgcgcgagac gatcacgtgc gcgtcggagg
    12841 cgctcgagct cctcacggcc gcgaatcgtc gcgctgacgc gcaacgggcg acctattggc
    12901 tggcggccgg ccacatcgcc agtgatgacc cggctcgggc gcgcatgctc ttcgaggagc
    12961 tcctcgcggc gaccgccgtc gaggacggtg accccgacct gcgcgtccgg atcctcgtcg
    13021 ccctcgcgac cgtcgaggtc tatgccgggg aatcgagcgc ggcgatcggc ctcctcgagg
    13081 aggcccgcgc gatcggggcc gacctcgacg accggcggcg ggcctcgttg ctgcactccc
    13141 tcgcccagtc gtatcggatg gcgggcgatc tcgagggtgc cgtccggacc gggatcgaga
    13201 gccttgccct gttccgcgcg gtggatgccc gcgccgagac ggcatcgatc gagaacgagc
    13261 tcgcgctgac ctatctcgga cttggcaacc tggccgcggc cgaacagcat gcccgggagg
    13321 cgcgcctggg catggagcgg gatccggatc aattccggct cgcccacctc ggcgacacgg
    13381 acgcccggat cgcgctggcc cgcggcgacc tggagctggc gggtcgccag gccacggccg
    13441 ccgccgagct cgccgccctg ctcggcaacc agaaggcgct cgtcgacgcc ctcctgacgc
    13501 tggcgcgcac ggcccgccgg cagggcgatc cggcgcttgc agccgcaact ctcgagcggg
    13561 cggcgacggc ggccgaggac ggaccgctcg cccggcttcg actcgtcctc ggcgaatgga
    13621 gcgagctggc ggccgacaac ggcgaccatg ccaccgccta cgagctgagc cgtcgagcgc
    13681 tggccctacg ctgagacgac cgtccggtcc gtgcggatcg ggcgggcccc cgaaggtcgg
    13741 gcccgcgtga cctggaggcg gctcagccgt agaagtcgac gaccagtcgg gtccgggtgc
    13801 tcgatcgcca gatgccgatg ccgatccagt ggtaggccgg gttcttcagg ttccgccaat
    13861 ggccgccgtt ggtcgacttc tcggcctgga acttgaggtg cgacgcaagg acggcggcct
    13921 tggccgtcga atagccgtcg cggcagccga tgttctcgcc ccagttccag cgggtgaagc
    13981 cggctcgacg aagccggtgg cccggatcgc cgtcggcggt gtgagagcac agcgctcggg
    14041 tcacgaggag cttggcgtac ggccgggaca ccttgtccga caagccgtag gaataggcga
    14101 tcggggcgac gtacgcgctg tagtggccgc tgccgtagcc gcggcacgtc ccgtcgctga
    14161 ggacccaacc cccggtccgg gtgcagttca cgagcccaag atagaaccgt tcgaccgaga
    14221 accacggctt gtcgagggtg gtcgtcgcca gggcgacgct ggacgatggg gcagtccgcg
    14281 tcgtcgtcgc ggcagcgaca cctggcgcag cggcgaggga tccgagcagc gacccggcga
    14341 cggccagccc gaggacgatc tgccggcgcg tccgtgcgcg ctgagcgatg cgagaggacg
    14401 cccgacgggc gccggtcgtg ggacgaatca agactcgtgc ctcctgtctg ccggacgtcg
    14461 cggcgaggac gcgacgacgg tggtcgaggc cgccgggcag gcgcaggggt tg
//
LOCUS       DATAIW010000254         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_104271, whole genome shotgun sequence.
ACCESSION   DATAIW010000254 DATAIW010000000
VERSION     DATAIW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104271"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1303
                     /locus_tag="VHM48_08095"
     CDS             245..1303
                     /locus_tag="VHM48_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2755408.1"
                     /translation="MYAVILAGGGGTRLHPLSRPECPKPFLPLLDDRSLLQGTVERLA
                     GLTDDITVVTDRRYERLVREQVPSVAVLLEPLGRNTAAAIALAALTIDRHQDDVMLVV
                     PADQTVERADLSRDVLRIAGEHLATGAFGVEDPLVTLGVTVDRPATDYGYLIPDLGRG
                     ADIDGLHAYPLVRFEEKPKPARAEELTRQEGVAWNAGIFLWRRRAIIAALGRYTGLLQ
                     SLGPMVATPSMLEHAYEAIQKPVSIDYAVMEGAAQNGQVVMASMDVGWSDLGSWSALL
                     TALGARGSGGVVQAGETVEVEHDDLIVRRIGGRLGVIAPPERGRMTATQPIAILRGTG
                     PDRPLVERLLERCSEPED"
     gene            1305..>1771
                     /locus_tag="VHM48_08100"
     CDS             1305..>1771
                     /locus_tag="VHM48_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755409.1"
                     /translation="MTLTGAAPTPTTIVFGTDGWRARIADEFTYDNVRRCAEGVAHYV
                     VERGETARGVVVAYDRRFSSEFFAMAAAEVLLAHEIPVAFAVHAVPTQMSSYEVVQRG
                     AAAGIVITASHNPWVDNGFKVKAPTGAAAGAEILGVIERRLAVNGGTGIDRRP"
BASE COUNT          274 a          633 c          609 g          255 t
ORIGIN      
        1 accacggaca gatcaccgtc ggctgagcgt gaccgaggcc attccgccag cgaatcggcg
       61 ggatcgtctc ctgcgcgggc tatccgagac gcgtttgttc tcctgggaag aacgagcgcc
      121 ggaagaacga gcgcccgaag aacggcacgc cccgaagcac gaacacccga accacgagcg
      181 ccccgaagaa cgagcacccc gtcgagcttc cggccgacgc ttccgcccct agactaggcg
      241 gtgcatgtac gcagtcatcc tggccggcgg cggagggacc cgcctccatc cactgagccg
      301 acccgagtgc cccaagccct tcctgccgct ccttgatgac cggtcgctcc tccagggcac
      361 cgtggaacga ctcgccgggc tgaccgacga catcacggtc gtgaccgatc ggcgctatga
      421 gcgtctcgtg cgcgagcagg tcccgagcgt ggccgtcctg ctcgagccac tcggcaggaa
      481 caccgccgca gccatcgcac tcgccgcgct gacgatcgac cgccaccagg acgacgtcat
      541 gctcgtcgtg ccggccgacc agaccgtcga gcgggccgat ctgtcccggg acgtcctgcg
      601 catcgcgggc gagcatctcg cgaccggcgc gttcggtgtc gaggacccgc tcgtgacgct
      661 gggcgtgacc gtggatcggc cggcgacaga ctacggctac ctcatcccgg acctcggtcg
      721 cggggccgac atcgacgggc tccacgccta cccgctcgtt cggttcgagg agaagccgaa
      781 gcctgcccgg gccgaggaac tgactcgcca ggagggcgtc gcctggaacg ccgggatctt
      841 cctgtggcgt cgccgggcga tcatcgcggc gctcggtcgg tataccggcc tcctccagtc
      901 gctcggtccg atggtcgcca ccccatcgat gctcgaacac gcttacgagg cgatccagaa
      961 gccggtctcg atcgactatg cggtcatgga gggcgctgcg cagaacggcc aggtggtcat
     1021 ggcctccatg gacgtgggct ggtcggacct cggatcctgg tcggcactcc tcaccgcgtt
     1081 gggtgcccgc ggaagcgggg gcgtcgtcca ggcgggcgag acggtcgagg tggagcacga
     1141 cgatctgatc gtccggcgga tcggcggacg gctcggggtc atcgcgccgc ccgagcgcgg
     1201 taggatgacc gccacgcagc cgatcgcgat cctgcggggg accggtcccg accgcccgct
     1261 cgtggagcgg ctcctcgaac gctgctccga accggaggat tgacgtgacc ctgaccggcg
     1321 ccgcgcccac gccgaccacg atcgtgttcg gcacggacgg ctggcgcgcg cggatcgccg
     1381 acgagttcac ctacgacaac gtccggcgct gcgccgaggg cgtcgcccac tatgtcgtcg
     1441 aacggggcga gacggcccgg ggtgtcgtcg tcgcctacga ccgccgcttc tcgtcggagt
     1501 tcttcgcgat ggccgcggcg gaggtgctcc tggcccacga gatcccggtc gcgttcgcgg
     1561 tccacgccgt cccgacccag atgagttcct acgaggtggt gcagcgcggc gccgcggccg
     1621 ggatcgtcat cacggccagt cacaacccgt gggtggacaa cggcttcaag gtcaaggccc
     1681 cgaccggcgc cgcggccggc gcggagatcc tgggcgtcat cgaacggcgg ctcgccgtca
     1741 atggcgggac cggcatcgat cgccggccgt t
//
LOCUS       DATAIW010000255         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_125669, whole genome shotgun sequence.
ACCESSION   DATAIW010000255 DATAIW010000000
VERSION     DATAIW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125669"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VHM48_08105"
     CDS             complement(<1..412)
                     /locus_tag="VHM48_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2755410.1"
                     /translation="MTMRSARRAIPFASLGLFAAAVIGTLIGFNILILHLTSDPLTDI
                     RTYYDAGTRLNQGLPLYAQAASTNDAEFSRYPPLLAILFRPLALLPFEAAAAIWETIV
                     VGSLVATIAVLRPGRRGWLVFGMLALPIIWSLTIG"
     gene            complement(612..1625)
                     /locus_tag="VHM48_08110"
     CDS             complement(612..1625)
                     /locus_tag="VHM48_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2755411.1"
                     /translation="MELIRRIRSVLPGGAAGTAGIRSRVVLRSLWGAVAVLWALGGIR
                     IFLFHISTDPFNDLHVYYDAAARLNAGLPLYPPGARIYPPLFSILFRPLALLPFELAA
                     VIWEAILLAALGLTLWRIGIRRPATWWAVLLLSIGIGWTLAIGQVEAIVALLLTIGSP
                     FTVALAGNIKLYPLAAGIYWLARRDWQALGRLAGWTAALVLLQLILDPADTLAFPGTL
                     APSQVASEVSSSGNLSPFVISPVVWLVCVAIEVAVAWRFGRTRWGWAAAVGLAVLAPP
                     RVFSYNLMTLLACLGGPDMVRADGDAGRASSPARLRLGVRRRDRDRDVCSPRTSQPTH
                     HPR"
     gene            1786..3483
                     /locus_tag="VHM48_08115"
     CDS             1786..3483
                     /locus_tag="VHM48_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2755412.1"
                     /translation="MRTPIALYLLALIARLVVIGHFPDPAYPDSYYYVDVARNLAAGH
                     GFNVDFVWIFAEVGGKIPALPVLPIPSNAHWMPLASLVQVPFIWLLGPTAVASAVPFA
                     LIGALAAPLTWAIARDAGTSRQVAVGAAVLVALPVMLLVFMVQPDNFSLYQPLVAGAL
                     WLAARGLRGDARAFALAGLLVGLATLSRNDGVFVGATVGLAFLWDRWRAWRSGGSRRP
                     AIPLWSAVACAALFAVVIAPWIVRQLAVFGSISPSTASGKVLFIRTIEEWNSITTPAN
                     LSWLLGQGIGPLVLSRIGGLIAAVSIFTTLACGVVLVLPLVVGAWSRRRSVWFGPVFV
                     YAAILFSFSALVSAVHVPGGTFIHSAVALVPHAFILVLEGIAVGVGWIAARRRAWNRE
                     TATRVFTTTAVGFGAVLTILVSFAVHSGWAQKRADRASLGAALAVFADADARVMSIDA
                     SGIEYATGHGGVVSPNDPIDAIREAATAYRTEWLILERDSIVAALAPVLTGQARPAWI
                     GAPVLTIAAADGGAPRAALYPICLSPADARCTIVAGGPVVASGPVVAGLAAQPEARP"
     gene            3564..>4112
                     /locus_tag="VHM48_08120"
     CDS             3564..>4112
                     /locus_tag="VHM48_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755413.1"
                     /translation="MFPKPEDTAYYVDVARNLLGGRGLVSDALWSYQTQPLVVPREAF
                     EVWLPLPTFAAAVPMAILGATFAAAQVSSVLIGALVPVLAWRLAADVAEERRLPIGRA
                     RTLAVGTGLTAAVSLPLILHSTLPDSTMPFAALTLGAGLLITRIRHFVEERREDAVHS
                     GAAARPVGFLVGLGVVLGLAALT"
BASE COUNT          556 a         1500 c         1449 g          607 t
ORIGIN      
        1 ggccgattgt caggctccag atgatcggca gcgccagcat cccgaacacc agccagcccc
       61 gtcggccggg ccgcaggacg gcgatcgtcg ccaccagcga cccgaccacg atcgtctccc
      121 agatcgccgc cgcggcctcg aacgggagga gggccagcgg ccggaagagg atcgccagga
      181 gcggcgggta gcgagagaac tcggcgtcgt tcgtgctcgc cgcctgggcg tacagcggca
      241 ggccctggtt cagccgcgtc ccggcgtcgt agtaggtgcg gatgtcggtc agcgggtcgc
      301 tggtcaggtg gaggatcagg atattgaagc cgatcagcgt cccgatgacg gcggccgcga
      361 acaggccgag cgaggcgaat gggatcgccc gccgtgcgga gcgcatcgtc acgaggcccg
      421 agcccctgcc acgatcgagc aagcgtccgc gtggttccgc cgggcacgac gatccatcgc
      481 ccggagaacg ggcgcaacga tcgagaattc gacgagtgtc cactggaacg gcatcgcgtc
      541 gccgatcgaa agcctgtccg ctgcgtcaga ggcggcgatc gtgaactcag ccacaagcgc
      601 ctggagccat gtcaccgggg gtggtgggtc ggctggctgg tccgcggcga gcagacatcg
      661 cggtcacgat cacggcgccg cacgccgagc cggagccggg ccggcgacga agctcgtccg
      721 gcgtccccat ccgcgcgcac catgtcaggc cctccgaggc aggccaggag cgtcatcagg
      781 ttgtacgaaa agacgcgcgg cggagcgagc acagccaggc cgaccgccgc ggcccatccc
      841 caacgggtgc gcccgaacct ccacgcgacg gccacttcga tggcgacgca gaccagccag
      901 acgactggcg agatgacgaa cggcgacagg ttcccgctcg atgagacctc ggaggcgacc
      961 tggcttgggg cgagcgtgcc gggaaaggcc aacgtgtcgg ccggatcgag gatcagctgg
     1021 aggaggacga gggccgccgt ccagccggcg agtcgcccga gggcctgcca gtcgcgacgg
     1081 gccagccagt agatccccgc cgccagcggg tagagcttga tgttcccggc cagcgcgacg
     1141 gtgaacggac tcccgatcgt gagcagcagg gcgacgattg cctcaacctg gccgatcgcg
     1201 agcgtccagc caatcccgat gctcaacaac aggaccgccc accacgtcgc cggcctgcgg
     1261 atcccgatgc gccagagtgt caggccgagt gccgcgagga ggatggcctc ccagatcaca
     1321 gcggcgagct cgaacggcag gagcgcgagc ggacggaaca ggatcgagaa gagcggagga
     1381 tagatgcgag cgcccggcgg atagagcggg agacctgcgt tcaagcgcgc ggcggcgtcg
     1441 tagtagacgt ggaggtcatt gaacgggtcg gtcgagatgt ggaacaggaa tatcctgatt
     1501 cccccgaggg cccagaggac cgccacggcg ccccagagcg agcgcaacac gacccgggat
     1561 ctgatcccgg ccgtcccggc ggcaccgccc ggcaataccg accgaatacg gcggatgagc
     1621 tccatgagcg ggaagtctac gcggggacgt ggacccgccg accacccgaa ggtcacccgg
     1681 atcgcccctc atcaggcgcg ccgcgtctcg cgaccacggt ccgggcggcc tagtccattc
     1741 gtaccagtcg gaacgcgccc gacccggtgc tacgctgcac cggccgtgcg gaccccgatc
     1801 gccctctacc tgctcgccct gatcgcgcga ctggtggtca tcggccactt cccggacccg
     1861 gcctaccccg attcgtatta ctacgtggac gtcgcccgga acctcgccgc cggccacggc
     1921 ttcaacgtcg acttcgtctg gatcttcgcc gaggtcggcg ggaagatccc ggcgctcccg
     1981 gtcctgccga tcccgtcgaa cgcccattgg atgccgctcg cctcgctcgt ccaggtcccg
     2041 ttcatctggc tcctcggccc gacggcggtg gcatcggccg tgccgttcgc cctgatcggc
     2101 gccctggccg cgccgctgac ctgggcgatc gcccgcgacg ccggcacgag ccgccaggtg
     2161 gccgtcggcg cggccgtgct ggtcgccctg ccggtgatgc tcctcgtgtt catggtccag
     2221 ccggacaact tctcgctgta ccagccgctc gtcgccggcg cgctctggct cgctgcgcgc
     2281 gggcttcgcg gcgacgcccg ggcgttcgcg ctcgcgggcc tgctcgtcgg gctcgcgacg
     2341 ctgtcgcgga acgacggggt cttcgtcggg gcgaccgtcg ggctggcgtt tctctgggat
     2401 cgctggcggg cgtggcgttc cggtggttcg cgccggcccg cgatcccgct gtggtcggcc
     2461 gtcgcctgcg cggcgctctt cgccgtcgtc atcgccccgt ggatcgtccg ccagctcgcg
     2521 gtcttcggct cgatctcgcc ctcgaccgcg tccggcaagg tcctcttcat ccggacgatc
     2581 gaggaatgga actcgatcac gacgccggcc aacctgtcgt ggctcctcgg ccaggggatc
     2641 gggccgctgg tcctgagccg gatcggcggc ctgatcgcgg cggtcagcat cttcacgacc
     2701 ctcgcctgcg gcgtcgtcct cgtcctgcca ctcgtcgtcg gggcgtggtc gcgtcggcgc
     2761 tcggtctggt tcggcccggt cttcgtctac gccgcgatcc tgttctcgtt ctccgccctg
     2821 gtgtcggcgg tccatgtccc gggcggcacg ttcattcact cggcggtcgc gctcgtgccc
     2881 cacgcgttca tcctcgttct cgaggggatc gcggtcggcg tcggctggat cgccgcccgc
     2941 cgccgggcgt ggaaccgcga gaccgcgacc cgcgtgttca ccacgaccgc ggtcgggttc
     3001 ggcgccgtgc tgacaatcct tgtctcgttc gccgtccaca gcggatgggc ccagaagcgg
     3061 gccgaccggg cgagcctcgg ggcggccctc gccgtcttcg ccgatgcgga cgcccgggtg
     3121 atgtcgatcg acgcgtccgg gatcgagtac gcgaccggcc acggcggagt cgtcagcccg
     3181 aacgacccga tcgacgcgat ccgcgaggcg gcgacggcct accgcacgga gtggctgatc
     3241 ctcgagcgcg acagcatcgt ggccgccctc gccccggtcc tgacggggca ggcgcgtccg
     3301 gcgtggatcg gcgcgcccgt cctgacgatc gcggccgcgg acggcggtgc gccgcgggcc
     3361 gccttgtatc cgatctgcct gagcccggcc gacgcccgct gcacgatcgt cgccggcgga
     3421 ccggtcgtcg ccagcggacc ggtcgttgcc gggctcgccg cgcagccgga ggcccggccc
     3481 tgagccgtcg cgaggcggtc gcgtcggccg cggccgtctt cctgatcgcg ctggcgatcc
     3541 gctggtacgc ggccagcctc gtcgtgtttc cgaagcccga ggacaccgcc tactacgtcg
     3601 acgtcgcccg caacctcctc ggcgggcggg gcctcgtcag cgacgccctg tggagctacc
     3661 agacccagcc gctcgtcgtc ccgcgcgagg ccttcgaggt ctggctgccg ctgccgacgt
     3721 tcgccgcggc ggtgccgatg gcgatcctgg gcgcgacgtt cgccgcggcg caggtcagct
     3781 cggtcctgat cggcgcgctc gtcccggtcc tcgcctggcg gctcgccgcc gacgtcgccg
     3841 aggagcgccg gctgccgatc ggccgcgccc ggaccctcgc ggtcgggacg ggactcacgg
     3901 ccgcggtctc gctgccgctg atcctccatt cgaccctgcc ggattcgacc atgccgttcg
     3961 cggccctgac cctcggcgcg gggctcctga tcacgcggat ccggcatttc gtcgaggagc
     4021 gccgcgagga cgcagtgcac tccggcgcgg ccgcccggcc ggtcggtttc ctcgtcgggc
     4081 tcggcgtcgt cctcggcctg gccgccctga cc
//
LOCUS       DATAIW010000256         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_125704, whole genome shotgun sequence.
ACCESSION   DATAIW010000256 DATAIW010000000
VERSION     DATAIW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125704"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..>2969)
                     /gene="uvrA"
                     /locus_tag="VHM48_08125"
     CDS             complement(430..>2969)
                     /gene="uvrA"
                     /locus_tag="VHM48_08125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2755414.1"
                     /translation="RVRVDGEMVDITDAPKLDKYKRHSIEVVVDRYIVRHADAPDGAE
                     RADDGRPIDPETGQPLPDPDKSRLADSIETALRLGEGVVLIAPASRDDEPPSFEERRY
                     SERFTCPYDGFTMDELEPRNFSFNSPHGACPACTGLGTRLEIDPDLVIPDKAKSLQQG
                     ALVPWAKMPNDASWRLKILEAICTSHGWDFKQPIRDLPPEAIEYILRAEKDEKVLVRY
                     RHERGENTYKATFEGIITNLERRYKETDSEYIKTELEKYMVTRPCPTCHGRRLKPEVL
                     AVTVDHQAISDVAVLSITDALDWIAALPPKLSDRERTIAVQVLKEITARLGFLVDVGL
                     DYLTLDRASVTLSGGEAQRIRLATQIGTTLMGVLYILDEPSIGLHQKDNAKLIATLTR
                     LRDLGNTVLVVEHDEETIRTADWVIDIGPGAGEHGGEIIANGTLEDLLAEPRSITGAF
                     LRGERSVAVPARRRKGNLKKLVIKGAREHNLRDVDFTVPLGTFTAITGVSGSGKSTLV
                     TDVLYRALARELNGSRETVGAHDSLKGAEHIDKIIEIDQSPIGRTPRSNPATYTGLFG
                     PIRELFAGVPESRVRGYQPGRFSFNVKGGRCENCKGDGILKIEMQFLPDVYVTCEVCK
                     GKRYNREALEIHYKGHSISDVLEMTIAEANDFFSAVPNVRTKLQTLVDVGLGYVHLGQ
                     PATTLSGGEAQRVKLATELSRRATGRTLYVLDEPTTGLHFADVEKLLEVLHRLVDGGN
                     TVLVIEHNLDVIKTADWIVDLGPAGGARGGLIIAEGTPEKVAATEGSATGEYLDRVLR
                     GEPLVPLSDVSFADEAGRGKGSNGTNGHGPAVKIGPSRKRAAAASTR"
BASE COUNT          412 a          978 c         1064 g          515 t
ORIGIN      
        1 gacgagggcc aggtgcgggt ggcgaagcgg gaacgtccgg ccgccgatcc gcgacacgcc
       61 gccgccgagg acgtggaact tctggcccgg ctgggtctcg gcggagtcgg cgaggaaggc
      121 gtattcgatg tccggcatga ccgcatcgta gccggtcgtg ctctgccgtc ggttgccgcc
      181 cgctcctcgt ctcgcgcgcc gctcggcgcc cggcaggatt cacgccccac gtgcacatgc
      241 gacgtgctgg accacgaggt gggcgttgga cgaggtcttc cggcccgaat ccgagcggaa
      301 ttggtcagtt tggcacgcgg cgcgagaaga atcggcagac cttgcgcggg gaggtgcaga
      361 actggtcgca tgtgcacgct cctggagaat cgccgcgccg gggccggggc gaccgccgcg
      421 tcactgcgtc tagcgcgtcg aagcggccgc cgcgcgcttc cggctcggcc cgatcttgac
      481 cgccggtccg tggccgttcg tgccgttcga gcccttgccg cggccggcct cgtcggcgaa
      541 cgacacgtcg gacagcggca cgagcggctc acccctgagc acccggtcga ggtattcgcc
      601 cgtggcggag ccctccgtcg cggccacctt ctccggcgtg ccctcggcga tgatcaggcc
      661 gccccgcgcg ccgccggccg gcccgaggtc gacgatccag tcggccgtct tgatcacatc
      721 gaggttgtgc tcgatgacga ggacggtgtt gccgccgtcg acgagccggt ggaggacctc
      781 gaggagcttc tcgacgtcgg cgaaatggag cccggtcgtc ggctcgtcga ggacatagag
      841 cgtcctgccg gtcgcccgcc gcgagagctc ggtcgcgagc ttgacccgct gcgcctcgcc
      901 gcccgagagg gtcgtcgccg gctgaccgag atggacgtag ccgagcccga cgtcgacgag
      961 cgtctggagc ttggtccgga cgttcggaac ggccgagaag aagtcgttcg cctcggcgat
     1021 cgtcatctcc agtacgtcgg agatcgagtg gcccttgtag tggatctcga gggcctcgcg
     1081 gttgtagcgc ttgcccttgc agacctcgca ggtgacgtag acatccggca ggaactgcat
     1141 ctcgatcttg aggatcccgt cgcccttgca gttctcacag cggccgccct tgacgttgaa
     1201 gctgaagcgc ccgggctggt agccccggac ccgcgactcg gggacgcccg cgaagagctc
     1261 acgaatcggg ccgaagagtc ccgtatacgt ggccgggttg gaacgcggcg tccggccgat
     1321 cgggctctgg tcgatctcga tgatcttgtc gatgtgctcc gcgcctttca gcgagtcatg
     1381 tgccccgacg gtttcccgcg agccgttgag ctcgcgggcg agggcccggt acaggacgtc
     1441 ggtcacgagg gtcgacttgc cgctgcccga cacgccggtg atggcggtga acgtgccgag
     1501 cggaacggtg aagtcgacat cccgcaggtt gtgctcgcgg gcgcccttga tcacgagctt
     1561 cttgaggttg cccttgcgcc gcctggccgg caccgcgacc gatcgctcac cgcggaggaa
     1621 ggcgccggtg atcgaacgcg gctcggcgag gagatcctcg agcgtcccgt tggcgatgat
     1681 ctcgccgccg tgctcccctg ccccgggccc gatgtcgatc acccagtcgg ccgtccggat
     1741 cgtctcctcg tcgtgctcca cgacgaggac ggtgttgccg aggtcgcgca gccgggtcag
     1801 ggtcgcgatc agcttcgcgt tgtccttctg gtggagcccg atcgacggct cgtcgaggat
     1861 gtagaggacg cccatcaggg tcgtgccgat ctgggtcgcc aggcggatcc gctgggcctc
     1921 cccacccgac agcgtcaccg acgcccgatc gagggtcaga tagtccagcc cgacatcgac
     1981 gaggaagccg aggcgggccg tgatctcctt gaggacctgg accgcgatcg tccgttcgcg
     2041 gtcggagagc ttcggcggaa gggctgcgat ccagtcgagc gcgtcggtga tcgagaggac
     2101 cgcgacatcg gagatcgcct ggtgatcgac cgtgaccgcg aggacctcgg gcttcaggcg
     2161 ccggccatgg caggtcgggc acggccgggt gaccatgtac ttctcgagct cggtcttgat
     2221 gtactcggag tccgtctcct tgtagcgccg ctcgaggttc gtgatgatcc cctcgaacgt
     2281 cgccttgtag gtgttctcgc cgcgctcgtg gcgatagcgg acgaggacct tctcgtcctt
     2341 ctcggctcgc aggatgtact cgatcgcctc gggcggcagg tcccggatcg gctgcttgaa
     2401 gtcccagccg tgcgaggtgc agatcgcctc gaggatcttg agccgccacg acgcgtcgtt
     2461 cggcatcttg gcccacggca ccagggcgcc ctgctggagg ctcttcgcct tgtccgggat
     2521 gacgaggtcc gggtcgatct cgaggcgcgt cccgagcccg gtgcaggccg ggcaggcgcc
     2581 gtggggcgag ttgaacgaga agttgcgcgg ctcgagctcg tccatcgtga acccgtcgta
     2641 cggacaggtg aaccgctcgc tgtagcggcg ctcctcgaag gacggtggct cgtcgtcgcg
     2701 tgacgccggg gcgatgagga ccacgccttc cccgagtcgc agggcggtct cgatcgagtc
     2761 ggccaggcgc gacttgtccg ggtccggcag cggctggccg gtctcgggat cgatcggccg
     2821 gccatcgtcg gcgcgctcgg cgccgtcggg tgcgtccgcg tgacggacga tgtagcggtc
     2881 gacgacgacc tcgatgctgt ggcgcttgta cttgtcgagc ttcggggcgt cggtgatgtc
     2941 gaccatctca ccgtcgaccc ggacgcgga
//
LOCUS       DATAIW010000257         7572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_45219, whole genome shotgun sequence.
ACCESSION   DATAIW010000257 DATAIW010000000
VERSION     DATAIW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45219"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHM48_08130"
     CDS             <1..328
                     /locus_tag="VHM48_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2755415.1"
                     /translation="IDILKIDRTFVSSVGDVVSPIARAIVDLGRTLGLQVIAEGIEHI
                     EQAHWLADMGCRYGQGYLYARPMGAAAIEALLAEGDGLARPIEPDRALAPAADASPRL
                     RVVGDE"
     gene            398..1165
                     /locus_tag="VHM48_08135"
     CDS             398..1165
                     /locus_tag="VHM48_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2755416.1"
                     /translation="MNELRLLSAFVQGLVAGVVALVVIALAAAVQSRAGVTFAELLTA
                     FAISVAVGAVSFLLPRIVEWQVRRSIGRGAAGSFAVHAVFGSGGPAGGAIDPPDAGDR
                     GTDRFDRFSDRARRVLTLAQDEAQRFNHAYIGTEHILLGLVREGDGVAAGVLTSMGID
                     LVKVRTAVEFIVGRGDRPVVGEVGLTPGAKRAIELAIDEARVLGHRHIGTGHLLLGLV
                     RAGDGMADEVLASFGVTLERTRTAVLAAIGDGKDAER"
     gene            complement(1219..1686)
                     /locus_tag="VHM48_08140"
     CDS             complement(1219..1686)
                     /locus_tag="VHM48_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755417.1"
                     /translation="MYPWIVLLHIAGAFMFAMSHGVAMWMLSELPKQRDPRRISALLD
                     LSGASLNGVYVGLLLLLVGGIWAGIDRGWFRFGWIWAAIVVLVAIVLAMYAIATPFYK
                     RLRIAVGIRAAGYPKDAPDPIPLPDAEVLAIAAASPVALLSAVGSAGCWSSSG"
     gene            complement(1711..2265)
                     /locus_tag="VHM48_08145"
     CDS             complement(1711..2265)
                     /locus_tag="VHM48_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755418.1"
                     /translation="MGAMPEPGPQHLAGRLLLASGALAAVGVAFLIAMAVAFAIGAGS
                     AGLAFGRINDVLVLVSYLLAAPSVVALRVLLRPQARRVGDIVALIGLTAIAAIVVLQF
                     LLIVGALTFEQQVGPVSLALLVLGGWFVLAGSIGSTSGVLPRGARMGLLAATYIGYPI
                     WAFWISRRLGSGNVGPTMIHPDGG"
     gene            complement(2301..2930)
                     /locus_tag="VHM48_08150"
     CDS             complement(2301..2930)
                     /locus_tag="VHM48_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755419.1"
                     /translation="MISDGVRIGAALLVAGPLIGTVCMFYPPFFRVWTVGRAEHLALV
                     VAHRRAWTMANVGFFVGTALTAGGLAALAGSLDLAAGPRAILVGGTAAYVIGGALWSS
                     VLAIRNRTTPLLGEMVASGTPTEPGEALLGAALGGLFAAFMVTTGASIMAIAAGLGVG
                     GAVAAPVASIAALIAALSIVGFIAVGDMLPAVLYPPTLVVGIAILAGWT"
     gene            complement(2930..3364)
                     /locus_tag="VHM48_08155"
     CDS             complement(2930..3364)
                     /locus_tag="VHM48_08155"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX2755420.1"
                     /translation="MIRSFARLAHPIASGGFVACAIVQVFLAGLGVFDDPRAFITHRE
                     FGYAFGWLTLIVLILALVGRSPRRITGLSILLLVQFALQSVFVALRGSLPAIAALHPL
                     NGFALLGVGIVLTRLAWQVRRADQTAPAGVPVGSLVTTRDAG"
     gene            complement(3385..3795)
                     /locus_tag="VHM48_08160"
     CDS             complement(3385..3795)
                     /locus_tag="VHM48_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755421.1"
                     /translation="MHKIVRSLVLALALGVIPLSAVAAHECVVVNRSATGNLHATVSG
                     VWMTVTLHDIYEETEFFGFPDLSEAQVAYAAALASSWGVPDSFTFRSDKTLLEHASGW
                     LKGDHATDGKGIDHFFDVYGERIVGALYAALANA"
     gene            complement(3929..4603)
                     /locus_tag="VHM48_08165"
     CDS             complement(3929..4603)
                     /locus_tag="VHM48_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755422.1"
                     /translation="MSEALDRVRVLIADDHASFRSGLQALLGTAADLLVVGEAADGDE
                     AVAAAIAVQPDVILMDLTMPGIDGIEATRRILDAAPHVAILVVTMGADDESVFAAIRA
                     GARGYVLKGAQRNELLRAIRSVAAGEAIFGPGIARRLVGYFSRPAPPAVERFPELTVR
                     EREILDLIAAGRSNAEITSRLGLSPKTVRNHVSNVFAKLDVRDRAEAIVRGREAGLGG
                     KNVRPG"
     gene            complement(4600..6924)
                     /locus_tag="VHM48_08170"
     CDS             complement(4600..6924)
                     /locus_tag="VHM48_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2755423.1"
                     /translation="MPRTSRLRRIATWLALAGSLGLGVGFIVVRATAPSDGARVEFYG
                     DGWSAAGIRIAPIDAPAPGLGAGNTVTTVAEQPIEAWLGQALAPWSRSVDRPATVGPI
                     SYRLADGASTRTVDVRWADPALGGTLLEGWSVILFSLAVAAIAAFVFARRPDEPAATA
                     LMIAACGAAGSSVPWLLGVTVSDVVVGWPFLLHALVTGPLYMLLWPAGLHLALVFPEP
                     ASIVRHRRWAIPAMYGGSLAAYGAAMLAARLGSASNLDWVATWPTIQVAIVVPLLTAA
                     LAMFVRSYRGALDPAFRTRIRWAMLGGGASAFLGLVGFMLPELILQRPLLPASWIGLT
                     ALPLPLGLALGILRDRLFDIDVVVRRTLVYGGLSVGVVACYLVVVSGIGVALGGQSGY
                     GASLFATGVAALVALPLRDVLQRAVSRLLYGERDEPVLAIRRLGQRLELATDPDRAFP
                     AIVDTVADSLRLPYVALEVIDETGRPAPTAERGRPSAEVVTLDLGHGSELVGRLLLGT
                     RAGERGFRPDELRLLEDLGRQAGAAIHALRLRDDLVRSRERLVFAREEERRRLRRDLH
                     DGLGPSLASIGLRAEAATATMATDPAEARRLLDELGVEVQTTLADVRRLVEGLRPPAL
                     DELGLVGAIRAQAARLEGGGTRSAILITVVASPLPLPDLPAAVEVAAYRIVAEALTNA
                     ARHAEAATCRVHLESGEELTIEIVDDGRGVPRGVVHGTGLESMSARAAELGGSIRIER
                     RRGGGTRVVARLPIVGRSPDAAVAPDGATGTVPR"
     gene            complement(7054..>7572)
                     /locus_tag="VHM48_08175"
     CDS             complement(7054..>7572)
                     /locus_tag="VHM48_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2755424.1"
                     /translation="FAQLIHYDDKVIHTEFSALMSKVMTNGNGRVKFPINEPASGKKK
                     SQIQEFLDYYLDAGTQHIALRTEDIVGTVRALRGLGVEFLGMPHAYYEQLAVRVGDVG
                     VPIDTLEELGIEADRDEEGYLLQIFTRPVQDRPTFFFEIIERHGSRGFGVGNFKALFE
                     AIEIEQARRGNL"
BASE COUNT         1190 a         2721 c         2705 g          956 t
ORIGIN      
        1 gatcgacatc ctcaagatcg atcggacgtt cgtctcgtcg gtcggcgatg tcgtgtcacc
       61 gatcgcccgt gcgatcgtcg atctcggccg gacgctcggg ctgcaggtca tcgccgaggg
      121 gatcgagcac atcgagcagg cccactggct ggccgacatg ggttgccggt acggccaggg
      181 ctacctgtac gcgcgaccca tgggcgctgc cgcgatcgag gccctcctcg ccgagggaga
      241 cggcctcgct cgcccgatcg agccggaccg ggctctcgca ccggcggccg acgcgagtcc
      301 gcgactgcgc gtcgtcgggg acgagtagtc ctcgatcgga cccgtcccgc gcgcggcggt
      361 gtcgcatgcc ccgagcgctg ctctctagac tggatcggtg aacgaactgc gtctcctttc
      421 ggcgttcgtc caggggctcg tcgcgggcgt cgtggccctc gtcgtcatcg cgctggcggc
      481 ggcggtccaa tcccgcgccg gcgtgacgtt cgccgagctg ttgacagcgt tcgcgatcag
      541 cgtggcggtt ggggcggtgt cattcctgct gccgagaatc gtcgagtggc aggtccggcg
      601 gtcgatcggg cgcggagctg ccggctcctt cgcggtccat gccgtcttcg ggagcggcgg
      661 tccagcgggc ggcgcgatcg atccgcccga cgcaggcgac cgcgggaccg accggttcga
      721 ccggttctcg gatcgggcgc ggcgcgtcct gactcttgcc caggacgagg cgcagcggtt
      781 caaccacgcc tacatcggga ccgagcacat cctcctcggc ctcgttcgcg aaggcgatgg
      841 ggtcgccgcc ggagtcctca cgtcgatggg catcgacctg gtgaaggtcc gcaccgcggt
      901 cgagttcatt gtcggccgcg gggatcggcc ggtggtcggc gaggtcgggc tgacgccggg
      961 cgccaagcgg gccatcgagc tcgccatcga tgaggcgcga gtgctcggac atcgccacat
     1021 cggcaccggg cacctcctgc tcggcctcgt ccgcgcgggc gacggcatgg ccgacgaggt
     1081 gctcgcgtcg ttcggggtga cgctcgagcg cacccggacc gccgtcctcg ccgcgatcgg
     1141 ggacggcaag gacgcggagc ggtgagtcat cggattccgc ggtcggtcgc ggggcgggtc
     1201 agaacggctt gacgaccatc agccagagga tgaccagcaa cccgccgagc cgacggcgct
     1261 gagcagcgcg accgggctcg cggcggcgat ggcgaggacc tccgcgtccg gcagcgggat
     1321 cgggtccggc gcgtccttcg gatagccggc cgcgcggatc cccacggcga tccggaggcg
     1381 cttgtagaac ggtgtcgcga tcgcgtacat cgcgagcacg atcgcgacga ggacgacgat
     1441 cgcggcccag atccagccga agcggaacca gccgcggtcg atcccggccc agattccacc
     1501 gacgaggagc agtaggagcc cgacatagac cccgttaagg ctggctccgg agagatccag
     1561 gagcgcgctg atccgcctgg ggtcacgctg cttcggcagc tcgctgagca tccacatcgc
     1621 cacaccgtgc gacatcgcga acatgaacgc ccccgcgatg tggagcagga cgatccaggg
     1681 gtacacgggc agcctccaag ggggccggcg tcagccgccg tccgggtgga tcatggtcgg
     1741 tccgacgttg ccggatccga ggcgccggct gatccagaac gcccagatgg gatagccgat
     1801 atacgtcgcg gcgagaagcc ccatccgggc accgcggggc agaacgccgc tcgtgctccc
     1861 gatcgatcct gcaagcacga accagccgcc gaggacgagc aacgcgagcg agacgggccc
     1921 gacctgctgc tcgaacgtca gcgccccgac gatgagcagg aactgcagga cgacgattgc
     1981 cgcgatcgcg gtgagcccga tcagagcgac gatgtcaccc acgcgacgcg cctggggtcg
     2041 gagcagcacc cggagcgcca ccacgctcgg agcggcgagc aggtagctga cgaggaccag
     2101 cacgtcattg atccgcccga aggctagacc tgcagacccc gcgccgatcg cgaacgcgac
     2161 ggccatcgcg atcaggaagg ccaccccgac cgccgcgagg gctccgctcg cgagcagcaa
     2221 gcggccggcc aggtgctgcg gcccgggctc cggcatcgcg cccatcggcg gcagcgtcag
     2281 gtcagtcgac ccggaccggc tcacgtccag cccgccagga tggcgatccc gacgacgagc
     2341 gtcggcgggt agaggacggc cgggagcatg tcaccgacgg cgatgaagcc gacgatcgac
     2401 agcgcggcga tgagggcggc aatcgaggcc accggggccg ccacggcgcc gcccacgccc
     2461 aaccccgcgg cgatcgccat gatcgacgcg cccgtcgtca ccatgaacgc ggcgaacagg
     2521 ccgcccaggg ccgcgccgag cagggcctcc ccaggctccg tcggcgtccc ggacgcgacc
     2581 atctccccga gaagcggggt cgtccggttc cggatcgcga ggaccgaact ccagagcgct
     2641 cctccgatca catacgccgc ggtaccaccg accaggatcg cccgcgggcc ggccgcgagg
     2701 tccagggatc ctgcgagcgc ggcgagtcca ccggcggtca gcgccgttcc gacgaagaac
     2761 ccgacgttgg ccatcgtcca ggcgcggcgg tgcgccacga cgagcgcgag gtgctcggcc
     2821 cggcccacgg tccaaacccg gaagaagggc gggtagaaca tgcagactgt cccgatcagc
     2881 gggcccgcca cgagaagcgc cgcgccgatg cgcacgccgt cgctgatcat cagccggcgt
     2941 ccctcgtcgt caccagcgag ccgaccggca cgcccgccgg ggcggtctgg tcggcccgcc
     3001 ggacctgcca cgccagccgg gtcaggacga tcccgacgcc gagcagggcg aagccgttga
     3061 gcgggtgcag cgcggcgatg gccggcagac tgccgcggag ggccacgaac accgactgga
     3121 gcgcgaactg gacgaggagg aggatcgaca ggccggtgat ccggcgcggt gaccgtccga
     3181 cgagggcgag gatcagcacg atcagggtca gccagccgaa cgcgtagccg aactcccggt
     3241 gggtgatgaa agcccgcgga tcgtcgaaga cgccgaggcc ggcaaggaac acctggacga
     3301 tcgcgcaggc cacgaacccg cctgatgcga tcggatgagc gaggcgagcg aacgatcgga
     3361 tcatggtcgg acctcccgcg ccggtcaggc gttcgcgagc gctgcgtaga gcgctccgac
     3421 gatccgctcg ccgtagacgt cgaagaagtg gtcgatgccc ttgccgtccg tggcgtggtc
     3481 gcccttgagc cagccgctcg cgtgctcgag cagggtcttg tcactgcgga acgtgaagct
     3541 gtccggcacg ccccacgacg acgcgagggc tgcggcgtag gcgacctgtg cctcggacag
     3601 gtccgggaag ccgaagaact ccgtctcctc gtagatgtca tggagcgtca ccgtcatcca
     3661 gaccccggac acggtggcat ggaggtttcc ggtcgcggag cgattgacca cgacgcattc
     3721 gtgggcggcg accgcgctga gcgggatcac gccgagggcg agcgccagga cgagactgcg
     3781 gacgatcttg tgcatctggt gacctccggg actggtgcca tgccgcgggc tcgcgatggc
     3841 ccgtctcgcc cagcctcgtt cggcgcacgt cccgccgtca tgggaccggt gtcccgatcg
     3901 gtgtcccgat tcgaggcccg ctccgggctc agcccgggcg gacgttcttg ccgcccagtc
     3961 ccgcctcgcg accgcggacg atcgcctcgg cccggtcgcg gacgtcgagc ttggcgaaca
     4021 cgttcgagac gtggttgcgg acggtcttcg gcgagagccc gagccggctc gtgatctcgg
     4081 cgttcgagcg gcccgcggcg atgaggtcga ggatctcgcg ctcccgcacg gtcagctccg
     4141 ggaatcgctc gacggccggc ggggccggtc gcgagaagta tccgacgagc cggcgcgcga
     4201 tcccgggacc gaagatcgcc tcccccgcgg cgacggaccg gatggcccgg aggagctcgt
     4261 ttcgctgggc gcccttgagg acgtagccgc gcgcgcccgc ccggatcgcc gcgaacacgg
     4321 actcgtcgtc ggcgcccatt gtgaccacga ggatcgcgac atgcggcgcc gcgtccagga
     4381 tccttcgggt cgcctcgatc ccgtcgatcc ccggcatcgt gaggtccatg aggatcacgt
     4441 ccggctggac cgcgatcgcc gcggcgaccg cctcgtcgcc gtcggccgcc tcgccgacga
     4501 ccagcaggtc cgcggccgtc ccgagcagcg cttgcaaccc ggaccggaac gacgcatggt
     4561 cgtccgcgat cagcacccgg acccggtcga gcgcctcgct catcgcggaa cggtccccgt
     4621 cgcgccgtcg ggggcgacgg ctgcgtcggg cgaccgaccg acgatcggca gccgcgccac
     4681 cacccgcgtc ccaccgcccc gccgccgctc gatccggatg ctcccgccga gctccgcggc
     4741 ccgggcgctc atcgactcga gcccggtgcc gtggaccacg cctcggggaa cgccgcggcc
     4801 gtcgtcgacg atctcgatcg tcagctcctc cccgctctcg aggtgaacgc ggcaggtcgc
     4861 cgcctccgcg tggcgcgccg cgttggtcag cgcctcggcc acgatccggt aggcggccac
     4921 ctcgacggca gccggcaggt cgggtagcgg aaggggcgac gccacgaccg tgatcaagat
     4981 cgccgaccgg gtgccaccgc cctcgaggcg tgcggcctgg gcccggatcg cgccgaccag
     5041 cccgagctcg tcgagcgccg gcggccggag accctccacg agccgccgga cgtcggcgag
     5101 cgtcgtctgg acctcgacgc cgagctcgtc gagcagccgt cgcgcctccg ccggatcggt
     5161 cgccatcgtc gcggtcgccg cctcggcccg cagcccgatc gaggcgaggg atggccccag
     5221 cccgtcgtgg agatcccgcc gcagccggcg gcgctcctcc tcccgggcga aaaccaggcg
     5281 ctcgcgcgat cggacgaggt cgtcgcggag gcgcagggca tggatcgccg cgcccgcctg
     5341 gcggccgaga tcctcgagca atcgcagttc gtcggggcgg aaccctcgct cgccggcgcg
     5401 ggtcccgagc agcaacctgc cgacgagctc cgagccatgg ccgaggtcca gcgtgacgac
     5461 ctcggccgag gggcgtcctc gctcggccgt cggggccggc cggccggtct cgtcgatcac
     5521 ctcgagcgcg acgtacggca gccggagcga gtccgccacg gtgtcgacga tcgccggaaa
     5581 ggcgcggtcc gggtccgtcg cgagctccag ccgctgcccg agccggcgga tcgcgagcac
     5641 cggctcgtcg cgctcgccgt acaggagccg actgaccgcc cgctgcagga catcgcgaag
     5701 gggcagagcg acgagcgccg cgacgccggt ggcgaacagg gacgccccgt agccgctctg
     5761 gccgcccagc gcgaccccga tccccgagac gaccacgagg tagcaggcca cgacgccgac
     5821 ggacaggccg ccgtagacga gcgtccggcg gacgaccacg tcgatgtcaa acaaccggtc
     5881 gcgcaggatc ccgagcgcaa gtcccaacgg gaggggcagc gccgtcagcc cgatccacga
     5941 agccgggagc agcggccgct ggaggatgag ctccggcagc atgaagccga cgaggccgag
     6001 gaaggcgctg gcgccaccac cgagcatcgc ccagcggatc cgggtccgga aggccggatc
     6061 gagcgcaccc cggtagctcc gcacgaacat tgccaaggcg gccgtgagaa gcggcacgac
     6121 gattgcgacc tggatcgtcg gccaggtcgc gacccagtcc aggttcgagg ccgacccgag
     6181 gcgggccgcg agcatcgccg cgccgtaggc cgcgagggat ccgccgtaca tggcggggat
     6241 cgcccagcgg cggtggcgga cgatcgatgc cggctcgggg aacacgagcg cgaggtgcag
     6301 gccggcgggc cagaggagca tgtacaacgg accggtcacg agtgcatgga ggaggaacgg
     6361 ccaaccgacg actacgtcgc tgaccgtcac tcccagcagc cagggcacgc tgctccccgc
     6421 tgcgccgcag gccgcgatca tcagcgccgt ggccgccggt tcgtcgggcc gacgggcaaa
     6481 gacgaacgcg gcaatggcgg caaccgcgag cgagaacagg atcacgctcc agccctcgag
     6541 cagcgtcccc ccgagcgccg gatccgccca tcggacgtcg actgtccggg tcgacgcccc
     6601 atcggcgagg cggtagctga tcggcccgac cgtcgccggg cgatcgaccg accgactcca
     6661 cggcgccagc gcctgtccga gccacgcctc gatcggctgc tcggcgaccg tcgtcaccgt
     6721 gttgccggcg ccgaggcccg gcgccggcgc gtcgatcggg gcgatccgga tccccgcagc
     6781 cgaccagccg tcgccgtaga actcgacgcg cgcgccgtcc gacggcgcgg tggcccggac
     6841 gacaatgaag ccgacgccca gcccgagcga tcccgccaac gcgagccacg tcgcgatccg
     6901 tcgaagccgg ctcgtccgcg gcatccgatc cctccgtgac ccccgtcacg acgtccgatc
     6961 cggcggcatc ctgccgcggg cgctcagtct acggccgcag ggacgtccga cggccgccgg
     7021 actggcgagc tgacgcgggg ccgtcaggac cgtctagagg ttgccccgcc gcgcctgctc
     7081 gatctcgatc gcctcgaaga gcgccttgaa gttgccgacg ccgaagccgc gcgagccgtg
     7141 ccgctcgatg atctcgaaga agaacgtcgg gcgatcctgg acgggccggg tgaagatctg
     7201 gaggagatag ccctcctcgt cgcgatcggc ctcgatcccg agctcctcga gcgtgtcgat
     7261 cgggacaccg acatcgccga cccgcaccgc gagctgctcg tagtaagcgt ggggcatgcc
     7321 gaggaactcg actccgaggc cgcgcagcgc ccggacggtc ccgacgatgt cctcggtccg
     7381 gagcgcgatg tgctgcgtcc cggcatcgag gtaatagtcg aggaactcct ggatctggct
     7441 cttcttcttg ccggacgccg gctcgttgat cgggaatttc acccggccgt tgccgttggt
     7501 catgaccttc gacatcaggg cgctgaactc ggtgtggatg accttgtcgt cgtagtggat
     7561 cagctgggcg aa
//
LOCUS       DATAIW010000258         7793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_104888, whole genome shotgun sequence.
ACCESSION   DATAIW010000258 DATAIW010000000
VERSION     DATAIW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104888"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VHM48_08180"
     CDS             complement(<1..279)
                     /locus_tag="VHM48_08180"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX2755425.1"
                     /translation="MAIDERIRPGPTIADVDPELWAAMEGERHRQHDKIELIASENYT
                     SAAVMEAQGSWLTNKYAEGLPGRRYYGGCEFVDVAEELARERALKLFPG"
     gene            complement(384..1061)
                     /locus_tag="VHM48_08185"
     CDS             complement(384..1061)
                     /locus_tag="VHM48_08185"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX2755426.1"
                     /translation="MTARIVADDDAGRAAAADVLRSGGIVALPTDTVYGIAVALATPG
                     GIEALFAAKRRPPDKGIMLLLDDAAQAPAIGQWSESAAALADAFWPGGLTVIVPQRPD
                     VPLPPALTGETATIGLRVPDHGAPRALAAAVGPIPTTSANVSGVPEARDAAGIVAQLG
                     DAVALVLDGGPAHGGPASTVVDCTVEPPRILRVGAVPVAEVERVLGRSVVRAADGPAA
                     PSPGVGR"
     gene            complement(1058..1996)
                     /locus_tag="VHM48_08190"
     CDS             complement(1058..1996)
                     /locus_tag="VHM48_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX2755427.1"
                     /translation="MTSPRPRPTPAFPIGLIALDLDGTLIGEDLVLGPRTVAAIGAAR
                     ARGVAVSIVTGRMTTSALPFARRLGLIDPIVAYQGALVRALPPAASDPHLGRILAHRP
                     LAPAAAREVIAWAKGVGLEPHVNHLERFVIQRDDPRAEDYSTFLGARAEVVEDLRAWL
                     RFPISKVIAVGPATDPIPESILAEGRRRFAGRAAVTISHPRFLEFLAPGVSKLVGVRH
                     LARRARVPLGRVLAVGDQFNDLEMIAGVGHGAAMPSAPAQVRAAARYLAPPVGEEGTA
                     QLIEQLVLVSPETAARNADALAAAHDAATDGVDSAA"
     gene            complement(1993..2922)
                     /gene="prmC"
                     /locus_tag="VHM48_08195"
     CDS             complement(1993..2922)
                     /gene="prmC"
                     /locus_tag="VHM48_08195"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX2755428.1"
                     /translation="MATIGDCIADATARLRESGSETARLDAELLLGWAVGADRTAIVA
                     HRDAPVGADAFTRFAAAVARRATGEPVAYIRGIKEFHGLAFAVDPRGLIPRPESEAIV
                     DAAVAEIMVRLAGRPAEVGAGHGPGGDPIRVADVGTGSGAIAVAVAVALRRRRVTLGR
                     HVTIAATDISPDALDLARENAVGHAAADGMRFIEADLLPPVLPNGGAPFDLVLANLPY
                     VRSDAIAGLPIAASFEPRLALDGGSDGLDVIRSLLGLLPEALAVDGVALLEIGADQGE
                     SGPAAAAALLPGWRATVQADLAGLARVLRVERA"
     gene            complement(2954..5344)
                     /locus_tag="VHM48_08200"
     CDS             complement(2954..5344)
                     /locus_tag="VHM48_08200"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX2755429.1"
                     /translation="MSQRPDAQPPSSPTRISREATRAGDSAVDLVDARVVPIQNATDL
                     NSVQVLKHGNLYLMTDPFGDIHPDSRGLGLYRSDTRLLSCCALRVGGVRPVLLQGSMG
                     ANFRGSIQLTNPSADRNPDAKVHPGADVAGRTIGISRDRLIGNDGMEERVRIVNHAER
                     DLAFAVELELGCDAADIFEVRGYPRPERGTLLPIAMTSTRATFRYDGLDGMRRSTHVA
                     FNAAADAHGPIDGRSDDAIAQGGSVGYHWDVHLAPGEKRDLSWTIWSTTVRVGEGHVS
                     DDERAQASERALRATVATAAGQTGSAALFPEPPLISADDGTAAYHAWSRGTTAIQTDH
                     ELFNLTLARSLADLRLLINDGPGPGQRYVAAGVPWFATLFGRDALISSFQALAFRPQI
                     ALETLAVLAAYQATEVDDWRDAEPGKMLHELRLGEMAGAGELPHTPYYGSVDSTPLWL
                     ILFGATFDWTGDRALLDRYWPNAIAALDWIDRFGDKDGDGLVEYQRRSARGLLNQGWK
                     DSGNAIRDREGRGPTMPVALAEVQGYVFDAKRRMAGLATVRGETDLARRLLAEAEVLR
                     QRFEERFWVEDQRYYAMALDGEKRQLDAIGSNAGHCLWSGIVSPARARSVADRLMSPA
                     MFSGWGIRTYAEGQPGYNPIGYHTGSVWPHDTSLIAAGLKRYGFNDESNRLVGRVFEA
                     AQQFDEYRLPELFCGFDRDESAQPVPYPVACSPQAWAAGASFLFVETMLGLRAHAAAN
                     ELELHHPTLPDWIGKITLTNLRIGDGSVDLLFHRWRGTTSAEVLRKVGDLSVTIRL"
     gene            complement(5328..6452)
                     /gene="prfA"
                     /locus_tag="VHM48_08205"
     CDS             complement(5328..6452)
                     /gene="prfA"
                     /locus_tag="VHM48_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX2755430.1"
                     /translation="MSGDADRTGLDARLREVVSQYEDVQAQLSTPEVSRDPDRLRTLG
                     RELSKLEPVVEAFQALEATRAELAGAREMRETGDADPDMKAMVADEIDRLIAAEDRLL
                     EEMKVLLLPRDPNDDRDVILEIRAGAGGEEAALFAAELYRMYVRYSERHRFTPELLSL
                     NETGIGGIKEAIVQVHGDGAYSRLKFEGGVHRVQRIPATESSGRIHTSTVTVVVLPEV
                     DDIEIEIDEEKDLRIDVKRSSGPGGQSVNTTDSAVRITHLPTGLVVEIQDEKSQHKNK
                     AKAMSVLRSRLYDRQQAKQREADSAARRSMVGGGDRSDKIRTYNFPQDRLTDHRIGLD
                     LSALPRVMDGEIDRLIDALVMADQADRLAEITGGDHVAAT"
     gene            complement(6449..7447)
                     /locus_tag="VHM48_08210"
     CDS             complement(6449..7447)
                     /locus_tag="VHM48_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2755431.1"
                     /translation="MPRYSYGGQALIEGVLMRGRDALAVALRHPDGRIVYATERLDSG
                     FHGSRWARLPFLRGLIVLYETLFVGTRWLVRSASVQAAGSGEEGDEGVELGKGSIALM
                     LGLTLLAGVGIFFLLPLLIASATTSNIQNGLVQHLVEGLIRVGIFLGYLLLISRAEDV
                     RRVFQYHGAEHMTIHALEAGDPLVVDAVRKYPTAHPRCGTEFLVVVIALSIVAFSLVG
                     RQDPVVMVGSRIALIPVIAAVGYEILKWGAKHRVNPLVRAIMYPGILVQMITTRQPTN
                     DMIEVAIVSMEQALQADGEALPLGSGTLPRDRMDVAAVVAAEAAAPVTIDPPLPGA"
     gene            complement(7457..>7793)
                     /locus_tag="VHM48_08215"
     CDS             complement(7457..>7793)
                     /locus_tag="VHM48_08215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX2755432.1"
                     /translation="GHTWNFGRQRDMHNFIEAPICAGRERTKDPVHPTQKPLRVLRQL
                     VEWGSNPGDLVLDPFMGVGSTGVAAVELGRRFIGIELEEGYVAAAQRRIDATAAPEPL
                     PIGASPVTG"
BASE COUNT         1087 a         2738 c         2878 g         1090 t
ORIGIN      
        1 cccggggaac agcttcaggg cccgctcgcg ggccagctcc tcggcgacgt cgacgaactc
       61 gcagccgccg tagtagcgcc tgccgggcag gccctcggcg tacttgttcg tgagccagct
      121 gccctgcgcc tccatgacgg cggcggacgt gtagttctcc gacgcgatca gctcgatctt
      181 gtcgtgctgg cggtggcgct cgccctccat cgccgcccac agctcggggt cgacgtcggc
      241 gatcgtcggg ccgggacgga tccgctcgtc gatcgccatc ctcgcgcgcc tcctcgtacg
      301 ggtcgtcgcc ggcccgggtt ccgcccatcc tccacaccgt accgggcgcg ggtcggtccg
      361 gggcgcgcgg ggatgtggtc cgactatctt cccacgccgg gcgacggtgc cgccggtcca
      421 tcggcggccc gtaccacgct ccggccgagc actcgttcga cctccgccac cggcacggcg
      481 ccgacgcgca ggatccgcgg cggctcgacg gtgcagtcga cgaccgtcga ggccgggccg
      541 ccgtgggccg ggccgccgtc gagcacgagc gcgacggcgt cgccgagctg cgcgacgatc
      601 ccggcggcgt cgcgggcctc gggaacgccg gacacgttgg ccgaggtcgt cggaatgggg
      661 ccgacggccg cggcgagggc gcgcggcgcg ccatggtcgg ggacgcggag gccgatcgtg
      721 gcggtctcgc cggtcagcgc tggcggcagc gggacgtcgg gtcgctgcgg gacgatgacg
      781 gtcagaccgc ccggccagaa cgcgtcggcg agcgcggccg ccgactccga ccactgcccg
      841 atcgccgggg cctgcgccgc gtcgtcgagg agcagcatga tccccttgtc aggcggccgg
      901 cgcttggccg cgaacagggc ctcgatcccg cccggggtcg cgagcgcgac ggcgatgccg
      961 tagacggtgt cggtcggcag ggccacgatc ccgccgcttc ggaggacgtc cgcggccgcg
     1021 gctcggccgg cgtcgtcgtc ggccacgatc cgggcggtca cgccgccgaa tccacgccgt
     1081 cggtcgctgc gtcgtgggcg gccgcgagcg cgtcggcgtt ccgtgccgcc gtctccgggg
     1141 agacgaggac gagctgctcg atcagctggg cggtcccttc ctcgccgacc ggcggggcga
     1201 ggtagcgggc cgcggcccgg acctgtgccg gcgccgacgg catcgccgcg ccgtgcccga
     1261 cgccggcgat catctccagg tcgttgaact ggtcgccgac cgccaggacc cgccccagcg
     1321 ggacccgggc gcgccgggcg agatggcgga cgccgacgag cttcgacacc cccggggcca
     1381 ggaactccag gaatcgggga tggctgatcg tgaccgcggc ccggccggcg aaccgccgcc
     1441 ggccctcggc gaggatcgac tcggggatcg ggtcggtggc gggcccgacg gcgatgacct
     1501 tcgagatcgg gaagcggagc cacgcccgga gatcctcgac gacctcggcc cgcgcgccga
     1561 ggaacgtcga atagtcctcg gcccgcgggt cgtcgcgctg gatgacgaat cgctcgaggt
     1621 ggttgacgtg cggctccagc ccgacgccct tcgcccaggc gatcacctcg cgggcggcgg
     1681 cgggggcgag cggtcggtgg gccaggatcc ggccgagatg ggggtcggat gcggccggcg
     1741 gcagggcccg gacgagggcg ccctggtagg ccacgatcgg gtcgatcaga ccgaggcgtc
     1801 gggcgaacgg cagggcgctg gtcgtcatcc ggccggtgac gatcgagacg gccacgccgc
     1861 gcgcccgggc ggcgccgatc gcggccacgg tccgcgggcc gaggacgaga tcctcgccga
     1921 tcaacgtccc gtccaggtcg agcgcgatca ggccgatcgg gaaggccggc gtgggccgcg
     1981 gccgcgggct cgtcacgccc gctccacgcg aaggacccgc gccagcccgg cgagatccgc
     2041 ctggaccgtc gcgcgccagc ccggaaggag ggcggccgcc gcggcgggcc cgctctcgcc
     2101 ctggtcggcg ccgatctcga ggagggcgac gccgtcgacg gccagcgcct cgggcagcag
     2161 gccgagcagc gatcggatga cgtcgaggcc gtcgctgccg ccgtcgagcg ccagccgcgg
     2221 ctcgaacgac gcggcgatcg gcagtccggc gatcgcgtcc gagcggacat agggcaggtt
     2281 cgcgaggacg agatcgaacg gcgccccgcc gttgggcagg accggcggca ggaggtcggc
     2341 ctcgatgaac cgcattccgt cggctgcggc gtgaccgacg gcgttctcgc gggccaggtc
     2401 gagggcatcc ggcgagatgt cggtagcggc gatcgtcacg tggcgaccca gcgtcactcg
     2461 ccgcctgcgg agcgcgactg cgaccgcgac cgcgatcgcc ccgctgccgg tgccgacgtc
     2521 ggcgacccgg atcggatcgc ctcccgggcc gtgacccgcc ccgacctcgg cgggccgccc
     2581 ggccagcctg accatgatct ccgcgaccgc cgcgtcaacg atcgcctcgc tctccggccg
     2641 ggggatcagc ccgcgcggat cgacggcaaa cgcgagcccg tggaactcct tgatcccgcg
     2701 gatgtaggcg accggctcac cggtcgctcg gcgcgcgacc gccgccgcaa atcgggtgaa
     2761 tgcgtcggcc ccgaccggtg cgtcgcgatg ggcgacgatc gcggtccggt cggcgccgac
     2821 ggcccagccg agcagcagct cggcgtcgag gcgcgccgtc tccgagccgg actcgcggag
     2881 gcgtgccgtc gcgtccgcga tgcaatcacc gatcgttgcc atccccgttc gccccgcctt
     2941 cccgcgccgt ggatcagaga cggatcgtga ccgacaggtc gccgaccttg cggaggacct
     3001 ccgcgctcgt cgtgccgcgc cagcgatgga acagcaggtc gaccgacccg tccccgatcc
     3061 gcaggttcgt cagggtgatc ttgccgatcc aatcgggcag ggtcggatga tggagctcca
     3121 gttcgttcgc cgctgcgtgc gcccgcaggc cgagcatcgt ctcgacgaac aggaacgagg
     3181 cgccggcggc ccaggcctgc ggcgagcacg ccaccgggta cggcaccggc tgggccgact
     3241 cgtcgcgatc gaagccgcag aagagctccg gcaggcggta ttcgtcgaat tgctgggcgg
     3301 cctcgaacac gcgacccacg agacggttcg actcgtcgtt gaagccgtag cgcttgaggc
     3361 cggcggcgat cagcgacgtg tcgtgaggcc agaccgatcc cgtgtggtag ccgatcgggt
     3421 tgtagcccgg ctgaccctcg gcataggtcc ggatccccca gcccgagaac atcgcggggc
     3481 tcatcagccg gtcggcgacg ctgcgggcac gggccggcga cacgatcccc gaccacaggc
     3541 agtgcccggc gttcgagccg atcgcgtcca gctggcgctt ctcgccatcg agggccatcg
     3601 cgtagtagcg ctgatcctcg acccagaagc gctcctcgaa ccgctggcgg aggacctcgg
     3661 cctcggcgag caatcggcgg gcgaggtcgg tctcgccgcg gacggtggcg aggccggcca
     3721 tccgccgctt ggcatcgaag acatagccct ggacctcggc gagggcgacc ggcatcgtcg
     3781 ggccgcggcc ttcccgatcg cggatcgcat tgccggagtc cttccagccc tggttgagga
     3841 ggccgcgcgc cgagcgccgc tggtactcga cgagcccgtc gccgtccttg tctccgaagc
     3901 ggtcgatcca gtcgagggcg gcgatggcgt tcggccagta gcggtcgagg agggcccggt
     3961 cgccggtcca gtcgaaggtc gccccgaaca ggatcagcca gagcggcgtc gaatcgaccg
     4021 agccgtagta cggcgtgtgc ggcagctcgc cggcgccggc catttcgccc agccgcagct
     4081 cgtggagcat ctttccgggc tcggcgtcgc gccagtcgtc gacctcggtc gcctggtagg
     4141 cggcgaggac ggcgagcgtc tcgagcgcga tctgcggccg gaaggcgagt gcctggaacg
     4201 aggagatcag ggcatcccgc ccgaacaggg tcgcgaacca cgggacgccg gccgcgacat
     4261 agcgctggcc aggcccgggt ccgtcgttga tcaggagacg gaggtcggca agcgatcggg
     4321 cgagggtcag gttgaagagc tcgtgatcgg tctggatggc cgtcgtgccg cgactccacg
     4381 cgtggtaggc cgccgtgccg tcgtcggcgc tgatcagcgg cggctccgga aagagggccg
     4441 ccgatcccgt ctgaccggcg gcggtcgcga cggtcgcgcg cagggcgcgc tcgctcgcct
     4501 gcgcccgctc gtcgtcggag acatggccct cgccgacccg gacggtcgtc gaccagatcg
     4561 tccagctgag gtcgcgcttc tcacccggag cgaggtggac atcccagtgg taaccgacgg
     4621 aaccgccctg ggcgatcgcg tcatccgatc gtccgtcgat cgggccgtgg gcgtcggccg
     4681 cggcgttgaa ggcgacatgg gtcgagcgac gcatcccgtc gaggccgtcg taccgaaacg
     4741 tcgcccgggt cgaggtcatt gcgatcggca gcagcgtccc gcgttccggt cgcggatagc
     4801 cgcggacctc gaagatgtcc gcggcgtcgc aaccgagctc gagctcgacg gcgaacgcca
     4861 gatcccgctc cgcatggttg acgatccgga cccgctcctc cattccgtcg ttgccgatca
     4921 ggcggtcacg cgagatcccg atggtccgcc cggcgacgtc ggcgccggga tggaccttgg
     4981 cgtccgggtt gcggtcggcg ctcggattcg tgagctggat cgagccgcgg aagttcgcgc
     5041 ccatcgagcc ctggagaagg accggcctga cgccgccgac gcgaagggcg caacacgaca
     5101 ggagtcgggt gtccgagcgg tacaggccga ggccgcgcga atcgggatgg atgtcgccga
     5161 acgggtcggt catcaggtac agattgccgt gcttcaggac ctgcacgctg ttgaggtcgg
     5221 tcgcattctg gatcgggacg acccgagcat cgaccaggtc gacggccgaa tcgccggccc
     5281 gcgtggcctc gcgggaaatc cgggtcgggg acgagggcgg ttgcgcgtca ggtcgctgcg
     5341 acatgatcac ctccggtgat ctcggcgagg cggtcggcct ggtcggccat gacgagggca
     5401 tcgatcagcc ggtcgatctc gccgtccatg actcgtggca gggccgacag gtcgagcccg
     5461 atccgatgat cggtcaggcg atcctggggg aagttgtagg tccggatctt gtccgaccga
     5521 tcgccgccgc cgaccatcga tcggcgggcc gccgagtcgg cctcgcgctg cttggcctgc
     5581 tggcggtcgt acagccgcga ccgaagcacg gacatcgcct tcgccttgtt cttgtgctgg
     5641 ctcttctcgt cctggatctc gacgacgagg ccggtcggga ggtgggtgat ccggacggcc
     5701 gagtccgtgg tattgaccga ctggccaccg ggcccggacg agcgcttgac gtcgatccgg
     5761 aggtccttct cctcgtcgat ctcgatctcg atatcatcga cctcgggcag cacgacgacg
     5821 gtcacggtcg acgtgtggat ccggcccgac gactcggtcg cggggatccg ctggacgcga
     5881 tggacgccgc cctcgaactt gagccggctg tacgccccgt ccccgtggac ctggacgatc
     5941 gcctccttga tcccgccgat gccggtctcg ttgaggctca gcagctccgg cgtgaaccgg
     6001 tgccgctccg agtagcggac gtacatccgg tagagctctg cggcaaagag cgccgcctcc
     6061 tcgccgccgg cgccggcccg gatctcgagg atcacgtcgc ggtcgtcgtt cgggtcgcgc
     6121 ggcaggagga gaaccttcat ctcctccagg agccggtcct ccgcggcgat caggcggtcg
     6181 atctcgtcgg cgaccatcgc cttcatgtcg ggatcggcgt cgcccgtctc gcgcatctcg
     6241 cgggcgccgg caagttccgc tcgggtggcc tcgagcgcct ggaacgcctc gacgaccggc
     6301 tccagcttcg agagctcgcg gccgagcgtc cgcagccggt cgggatctct ggacacttcc
     6361 ggcgtcgaca gttgcgcctg gacgtcctcg tactggctga cgacctcgcg cagccgagca
     6421 tcgaggccgg tccggtccgc gtcgccgctc atgctcccgg cagcggcgga tcgatcgtga
     6481 ccggcgccgc ggcctcggcg gcgacgaccg cggccacatc catgcgatcg cgcggcaggg
     6541 tgcccgagcc gagggggagg gcctcaccgt cggcctggag ggcctgctcc atcgacacga
     6601 tcgcgacctc gatcatgtcg ttcgtcggct ggcgggtcgt gatcatctgg acgaggatcc
     6661 cgggatacat gatcgcccgg acgagggggt tgacgcggtg cttcgcgccc cacttgagga
     6721 tctcgtagcc gaccgcggcg atgaccggga tcagggcgat ccggctgccg accatcacga
     6781 ccggatcctg gcgcccgacg aggctgaagg cgacgatcga gagggcgatg acgacgacga
     6841 ggaactcggt gccgcaacgg gggtgggcgg tcgggtactt gcggaccgcg tcgacgacca
     6901 gcgggtcgcc cgcctcgagg gcgtggatcg tcatgtgctc ggcgccgtgg tactggaaga
     6961 cgcgccggac gtcctcggcc cgcgagatca ggagcaggta gccgaggaag atgccgaccc
     7021 ggatcagccc ctcgacgaga tgctggacga ggccgttctg gatgtttgag gtcgtggcgc
     7081 tcgcgatcag cagcggcagc aggaagaaga tcccgacccc ggcgagcagc gtcagcccga
     7141 gcatcagcgc gatcgagcct ttgccgagct cgacgccctc gtcgccctcc tcgccgctgc
     7201 ccgccgcctg gaccgacgcg ctccggacaa gccagcgtgt gccgacgaac agggtctcgt
     7261 acaggacgat cagcccgcgc aggaatggca gcctcgccca gcgtgagccg tggaagcccg
     7321 agtcgagccg ctcggtggcg tagacgatcc ggccgtcggg gtggcggagg gcgacggcga
     7381 gcgcgtcgcg accgcgcatg agcacgccct cgatcagggc ctggccgccg tacgaatatc
     7441 gaggcatccc ggaagtctag ccggtgacgg ggctggcgcc gatcggaagg ggctccgggg
     7501 ccgctgtcgc gtcgatccgg cgttgcgccg ccgccacgta cccctcctcc agctcgatcc
     7561 cgatgaaccg ccggccgagc tccaccgccg cgacgccggt cgagccgacg cccatgaacg
     7621 ggtcgaggac gagatcgccc gggttcgagc cccattcgac gagctggcgc agcacccgca
     7681 gcggcttctg ggtggggtgg acgggatcct tcgtccgctc gcggccggcg cagatcggcg
     7741 cctcgatgaa gttgtgcatg tcgcgctgcc gcccgaagtt ccaggtgtgg ccc
//
LOCUS       DATAIW010000259         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_104942, whole genome shotgun sequence.
ACCESSION   DATAIW010000259 DATAIW010000000
VERSION     DATAIW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104942"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VHM48_08220"
     CDS             <1..890
                     /locus_tag="VHM48_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2755433.1"
                     /translation="STTPLSALLFAEVCREADLPPGVVNIVTGPGEIGMALVTNPGVD
                     KVAFTGSTEIGKRIARGVAGTDKALTLELGGKAANIVFDDAPLDQAVEGIVNGIYFNQ
                     GEVCCAGSRLLAQESIVGPLIDKLKDRLATIRVGDPLDKNTDVGAINSKAQLEKITEL
                     VASGVAEGAELYQPACDLPERGYFFRPTLFTNVAQSHRIAREEIFGPVLSVLTFRTPD
                     EAVEKANNTVYGLSAGIWTDKGSRILEMAKRMRAGVVWANTFNRFDPTSPFGGYKESG
                     FGREGGMHGLHAYVRLEDR"
     gene            890..1783
                     /locus_tag="VHM48_08225"
     CDS             890..1783
                     /locus_tag="VHM48_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2755434.1"
                     /translation="MGSPARVEVRKTYKLYIGGAFPRTESGRSYLVSGADGAPLANAC
                     RASRKDVRDAVRAARKAFDGWAARTAMNRGQVLYRVAELMEGRREQFVAEVEAAEGLK
                     PTAARAVVDRAIDRWVWYAGWADKIGQVLGSSNPVAAPYFNFTIPEPTGVVGIVAPET
                     SSLLGLVSRLAPPLVTGNAVVLIASERRPLPAVTLSEVLATSDVPGGVVNVLTGRKAE
                     LVPVLAGHADVDALDTWGVPDEMRTEVEILAAEDIKRLSRRPTKVVEARFDWLDDAAT
                     ERPEWIAAFLEMKTVWHPIGG"
BASE COUNT          281 a          628 c          620 g          265 t
ORIGIN      
        1 cctccacgac cccgctctcc gcactgctct tcgccgaggt ctgccgcgag gccgacctgc
       61 cacccggggt cgtcaatatc gtgaccggcc cgggcgagat cgggatggcg ctggtgacca
      121 atcccggtgt cgacaaggtc gcattcaccg gctccacgga gatcgggaag cggatcgcca
      181 ggggcgtcgc gggcacggac aaggcgctga ccctcgagct cggcgggaag gccgccaaca
      241 tcgtgttcga cgacgcgccg ctcgaccagg ccgtcgaggg catcgtcaac ggcatctact
      301 tcaaccaggg cgaggtgtgc tgcgccggct cgcggctgct ggcccaggaa tcgatcgtcg
      361 gaccgctcat cgacaagctc aaggaccgcc ttgcgacgat ccgggtcggc gacccgctcg
      421 acaagaacac cgacgtcggg gcgatcaatt cgaaggccca gctcgagaag atcacggaac
      481 tcgtcgcctc gggcgtggcg gagggcgcgg agctctacca acccgcctgc gatctcccgg
      541 agcgcggcta cttcttccgg ccgacgctct tcacgaacgt cgcccagagc catcggatcg
      601 cgcgcgagga gatcttcggg ccggtcctgt cggtcctgac cttccggacc ccggacgagg
      661 cggtcgagaa ggccaacaac acggtctacg gcctgtcggc cggcatctgg accgacaagg
      721 gctcgcgcat cctggagatg gccaaacgga tgcgggccgg cgtggtctgg gccaacacgt
      781 tcaaccgctt cgacccgacc tcgccgttcg gtggctacaa ggagtccggt ttcgggcgcg
      841 agggcgggat gcacggcctc cacgcctacg tccggctcga ggaccgctga tggggtcgcc
      901 ggctcgcgtc gaggtccgca agacctacaa gctctacatc ggcggagcct tcccgcggac
      961 ggagtcgggc cgctcctatc tcgtctcggg cgcggatggc gcaccgctcg cgaacgcctg
     1021 tcgcgcctcc cgcaaggacg tgcgcgatgc cgtccgggcg gcccgcaagg cattcgacgg
     1081 ctgggccgcc aggaccgcca tgaaccgcgg ccaggtcctg taccgcgtgg cggaactgat
     1141 ggagggccgg cgcgagcagt tcgtcgccga ggtggaggct gccgaaggcc tcaaaccgac
     1201 ggccgcgcgg gcagtcgtcg atcgcgccat cgaccgctgg gtctggtacg ccggctgggc
     1261 cgacaagatc ggccaggtcc tggggtcgtc caacccggtc gcggcgccgt acttcaactt
     1321 cacgatcccg gagccgaccg gtgtcgtggg catcgtcgca ccggagacct cctcgctcct
     1381 tggcctcgtc agccggctgg caccgccgct ggtgaccggc aatgccgtcg tcctgatcgc
     1441 gagcgagcgg cggccactgc cggcggtgac cctgtccgaa gtcctcgcca catccgatgt
     1501 cccgggtggc gtcgtcaacg ttctgaccgg tcgcaaggcc gagctcgtgc ccgtgctggc
     1561 cggacatgcg gacgtcgatg cgctcgacac ctggggggtc ccggacgaga tgcggacgga
     1621 ggtcgagatc ctcgccgcgg aggacatcaa gcgcctgtcg cgccgcccga cgaaggtcgt
     1681 cgaggcgcgc ttcgactggc tcgacgacgc cgccacggag cgccccgaat ggatcgccgc
     1741 cttcctcgag atgaagacgg tctggcaccc gatcggcggc tgacgatggc cgtg
//
LOCUS       DATAIW010000260         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_164784, whole genome shotgun sequence.
ACCESSION   DATAIW010000260 DATAIW010000000
VERSION     DATAIW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164784"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VHM48_08230"
     CDS             complement(<1..485)
                     /locus_tag="VHM48_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755435.1"
                     /translation="MTTATPPPARPGWRRLITAERASQIRTVTVLPIIALVLAVIVGS
                     LFIYLADAIKNKGAIDLGRPVTAYLALIGGSLGGFDSIIETLKQAAPLVLGGLAVGLG
                     FKAGLFNIGVQGQLLMGVLGSVAVGVALADQPVFIAAPIAFIVGTLAGAAMGFVPGFL
                     KA"
     gene            complement(482..2107)
                     /locus_tag="VHM48_08235"
     CDS             complement(482..2107)
                     /locus_tag="VHM48_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755436.1"
                     /translation="MTNAQEIVSDQPVHAPALEMRGITKRYPGVVANDRIDLEVRPGE
                     IHALLGENGAGKTTLMNILYGLARPDEGEILIDGAPVKITGPSDAIARGINMVHQHFM
                     LVPVLSVAENILLGEETMANAIFLDEKESKRRISDLGKRFGFEIDPEIKVGDLSVGWQ
                     QRVEILKALYRNAKVLVLDEPTAVLTPQETEEIFTVLRRLAGEGHSIVFISHKLNEVL
                     AIADRITIIRRGRVVGQRLPSETDEEDLATLMVGRDVQLVVDRGESHPGDVRLQVRGL
                     KVRDDRGTEAVRGVDFEIRAGEIFGIAGVAGNGQDELVEAIVGLRKPAEGTVSLDGND
                     VTDASAREMTNLGTGYVPADRHRFGLVLSFTIADNLVLTDYYRAPYARGIVRNDDAIE
                     ERGATAVKEYDIRTPSATALAGTLSGGNQQKLVVARELDGDLRLLVLDQPTRGLDVGS
                     IEFIHRQAIKKRDAGSAVLLVSAELDEVLELSDRVAVMYRGQIVDVVDGRTADRNLVG
                     MLMATGLRHEAAIAVGAVPPAPPPAAAASGAPS"
     gene            complement(2225..3415)
                     /locus_tag="VHM48_08240"
     CDS             complement(2225..3415)
                     /locus_tag="VHM48_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2755437.1"
                     /translation="MHKRSFGLSLFGGLAIIVAACGNGATPSPSAASQPPASAPASAA
                     ASTPASAPASQAATSKLKIGVVTDIGTLNDKNFNEYSFKGAQEGAAAIGAVAPVSIVP
                     TASTEYASSIKSFIDQKFDVILTVGFNLGSATAQAAHDNPDLWFVGFDQSPICVTPEG
                     LPDATFACKGDAKTLLPHYVSLQFAEDQAGYLAGIVAAGASKSGTIGAIGGSSICAPC
                     VRYIQGYELGAKSVNAAIKVVTAYVTNDFSAAAFQDQAGGKTYAENYLAQNKDVDVLF
                     QVAGLTGNGVLDAACAAKINGIGVDVDQFLSYPNAGPCLLTSAEKNLQLAISTAIQAV
                     AAGTATPGDNLWNAANDGIAVSEGHDKASAWAADTQAKLDAALAAMKAGTLTTCPANC
                     GSAK"
     gene            3645..4574
                     /locus_tag="VHM48_08245"
     CDS             3645..4574
                     /locus_tag="VHM48_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2755438.1"
                     /translation="MTTPEGPLAPVPGGRTASGIAFDDEGSGPPLALVHAGVADRRMW
                     SGLAAELANRHRVIRHDVRGVGESLPPSGPWSHHTDLLGLLDELLITRAHVVGASMGA
                     GIAVEAALARPGAVASLVLVAPGGALMGEAPDSIRPIWAAEVAALDRGDLDAAVEINL
                     RAWVDGPLRPADAVGPEVRAFIGRMQREAFELPEWDPEHAPEHELTPPAAERLAELTC
                     PILVVVGAADDPAIITISERIVAAARRARLVVLPGVGHMLSLEEPAVFTELLSGFLAD
                     VASGRFDGEAVVVPASSGSGPGDAVLPATAFEP"
BASE COUNT          697 a         1584 c         1675 g          744 t
ORIGIN      
        1 gccttcagga agcccggcac gaagcccatc gcggcgccgg ccagcgtccc gacgatgaac
       61 gcgatcgggg cggcgatgaa gacgggctga tcggccaggg cgaccccgac cgcgaccgag
      121 ccgaggacac ccatcaggag ctgaccctgg acgccgatgt tgaacagtcc ggccttgaac
      181 ccgaggccga ccgccaggcc gccgaggacc agtggggcag cctgcttgag ggtctcgatg
      241 atcgagtcga agccgccgag cgacccgccg atcagagcga ggtacgccgt gaccggcctg
      301 cccaggtcga tggcgccctt gttcttgatc gcatcggcga ggtagatgaa gagcgacccc
      361 acgatgaccg ccaggaccag cgcgatgatc ggcaggacgg ttaccgtccg gatctggctg
      421 gcgcgctccg cggtgatcag ccgtcgccac cctggtcggg cggggggcgg cgtcgcggtg
      481 gtcatgacgg tgcccctgac gccgctgccg cgggcggcgg cgcgggcggc acagctccga
      541 ccgcgatcgc cgcctcgtgc cggagcccgg tcgccatcag cattccgacc aggttccgat
      601 cggccgtccg accgtcgacg acgtcgacga tctggccgcg atacatcacg gcgacccggt
      661 cgctcagctc gagcacctcg tcgagctcgg ccgagacgag gagtacggcg gatccggcgt
      721 cgcgcttctt gatcgcctgg cgatggatga actcgatgct gccgacgtcg aggccgcggg
      781 tcggctggtc gagcacgagc aggcgaaggt cgccgtcgag ctcgcgcgcg accacgagct
      841 tctgctggtt cccacccgag agcgtaccgg cgagcgccgt cgccgagggg gtccggatgt
      901 cgtactcctt cacggccgtc gccccccgct cctcgatggc gtcgtcgttg cggacgatgc
      961 cccgggcgta cggcgcccga tagtagtcgg tcagaacgag gttgtcggcg atcgtgaacg
     1021 agagcacgag cccgaaccgg tgccggtcgg ccgggacata gcccgtgccg aggtttgtca
     1081 tctcccgggc gctggcatcg gtgacgtcgt tgccgtcgag actgaccgtg ccctcggccg
     1141 gcttgcgcag gccgacgatc gcctcgacga gctcatcctg gccattgccc gcgacgccgg
     1201 cgatcccgaa gatctcgcca gcccggatct cgaaatcgac cccgcgcacg gcctcggtcc
     1261 cgcggtcgtc gcggactttc aggccgcgga cctgaagacg aacgtcgccc gggtgcgact
     1321 cgccgcggtc gaccacgagc tggacgtccc gcccgaccat cagcgtcgcc aggtcctctt
     1381 cgtcggtttc cgacggcaga cgctgaccga cgacccggcc gcgccggatg atcgtgatcc
     1441 ggtcggcgat cgcgaggacc tcgttcagct tgtggctgat gaagacgatg ctgtggccct
     1501 ccccggcaag gcgccgcagg accgtgaaga tctcctcggt ctcctggggc gtcaggacag
     1561 ccgtcggctc gtcgaggacg aggaccttcg cgttgcggta gagggctttg aggatctcga
     1621 cccgttgctg ccagccgacg gacagatcgc cgaccttgat ctcggggtcg atctcgaacc
     1681 cgaaccgttt gccgaggtcg ctgatccgac gcttggattc cttctcgtcg aggaagatcg
     1741 cgttcgccat cgtctcctcg ccgaggagga tgttctcggc cacggacagg acagggacga
     1801 gcatgaagtg ctggtggacc atgttgatcc cacgggcgat cgcgtcgctc gggccggtga
     1861 tcttgaccgg cgcgccgtcg atcaggatct cgccctcgtc cgggcgggcg aggccgtaga
     1921 ggatgttcat cagggtcgtc ttgccggcac cgttctcgcc gagcagggcg tgaatctcgc
     1981 ccggacggac ctcgaggtcg atccggtcgt tggcgacgac gccagggtaa cgcttcgtga
     2041 tcccgcgcat ctccagtgcc ggggcgtgga cgggctggtc gctgacgatt tcctgggcgt
     2101 tcgtcacgac gatcgcattc ctcagctgac ggagcgggga ggcggctggc cggtgtagct
     2161 gaaagggtac agaaaagcgg gccggccgag tcgcctcgac cggcccgctc tgatggtccg
     2221 aagcctactt ggccgagccg cagttggcag ggcaagtggt gagggtcccg gctttcatcg
     2281 ccgcaagagc ggcgtccagc ttggcctgcg tgtccgcagc ccaggccgag gccttgtcgt
     2341 ggccctcgga gacggcgata ccgtcgtttg cggcgttcca gaggttgtca cccggtgttg
     2401 ccgtaccggc cgcgacggcc tggatggccg tagagatcgc gagctggagg ttcttctccg
     2461 cactcgtgag caggcaggga ccggcgttcg ggtaggacag gaactgatcg acgtcgacgc
     2521 cgatcccgtt gatcttcgcc gcgcaggcgg cgtcgaggac gccgttgccc gtgaggcccg
     2581 cgacctggaa caggacgtca acgtccttgt tctgggcgag gtagttctcg gcataggtct
     2641 tgccaccggc ctggtcctgg aaggcggccg ccgagaagtc attcgtcacg taagcggtga
     2701 cgaccttgat ggccgcgttg accgacttgg caccgagctc atagccctgg atgtagcgaa
     2761 cgcacggagc gcagatgctc gacccgccga tcgcgccgat cgtcccactc ttggaagctc
     2821 ccgcggcgac gatgccggcg agatagccgg cctgatcctc ggcgaactgg agcgagacat
     2881 agtgcggaag gagcgtcttc gcgtcgccct tgcaggcgaa cgtcgcgtcg gggaggcctt
     2941 cgggggtcac gcagatcggg ctctggtcga agccgacgaa ccacagatcg gggttgtcgt
     3001 gggcggcctg ggcggtcgca ctccccaggt tgaagccgac cgtcaggatc acgtcgaact
     3061 tctggtcgat gaacgacttg atcgaggacg cgtactcggt ggaggccgtc gggacgatcg
     3121 agacgggagc gaccgcgccg atcgcggcgg caccttcctg agcgcccttg aacgagtact
     3181 cattgaagtt cttgtcgttc agcgtcccga tgtcggtgac gacgccgatc ttgagcttgg
     3241 atgtcgccgc ctgcgatgcg ggcgccgagg ccggcgttga cgcggccgcc gaggccgggg
     3301 ccgacgccgg gggctgtgac gcggcggacg ggctcggggt ggccccgttc ccgcacgccg
     3361 ccacgatgat ggccaggccg ccaaacagcg acaggccaaa cgagcgcttg tgcatgtgtc
     3421 ctcctccagc atcgagatcc gtccggccga ggaattcgga tggtccgtgg cggtctcgaa
     3481 ggaacgttcg ctccgcggag cgagcctcgg cgcatcctac cgacggcgga tcggccgggc
     3541 aaatgcgaca cgacgccgca cgggcatggt cctgatccat gacaagaggg acgcgccgac
     3601 cacaaggata ggccccggga cgggccgctg catactgcgt cgacatgacc acgcccgaag
     3661 gccctctcgc gccagtgccc ggcggccgga cggcgagcgg gatcgccttc gatgacgagg
     3721 gctcgggtcc gccgctggcg ctcgtccacg ccggcgtcgc cgatcggcgg atgtggtccg
     3781 gcctcgcggc cgagctggcg aaccgccacc gggtgatccg ccatgatgtc cgcggcgtgg
     3841 gcgagtcgct cccaccgagc gggccgtggt cgcatcacac ggacctcctc gggctgctcg
     3901 acgagctgtt gatcacgcgc gcccacgtcg tcggagcgtc gatgggcgcg gggatcgcgg
     3961 tcgaggccgc gctcgcccgc cccggagccg tcgcgtcgtt ggtcctcgtg gcccccggcg
     4021 gcgcgctgat gggcgaagcg cccgattcga tccgcccgat ctgggcagcc gaggtggcgg
     4081 ccctcgaccg tggcgatctc gatgcggcgg tcgagatcaa cctgcgggcc tgggtcgacg
     4141 gaccgctgcg cccggccgat gccgtcggcc cggaggtccg ggcgttcatc ggccggatgc
     4201 agcgcgaggc cttcgagctg ccggagtggg atcccgagca cgcgcccgaa catgaactga
     4261 cgccgcccgc ggccgaacgc ctcgccgagc tgacgtgccc gatcctcgtc gtcgtcggcg
     4321 ctgccgacga cccggcgatc atcacgatct ccgagcggat cgtggccgcg gcgcgccggg
     4381 cgcgtctcgt ggtgttgccc ggcgtcggcc acatgctcag cctggaggaa ccggccgtct
     4441 tcaccgagct gctctcgggt tttctggcgg atgtcgcgag cgggcggttc gacggtgagg
     4501 cggtcgtcgt gccggcctcg agcggctccg gaccgggcga cgccgtgctg ccggccacag
     4561 cgttcgagcc gtgagacggc caaccacgac gcgccaggct gccctccgct acatcgccct
     4621 cggggacagc tacacgatcg gcacgaccct gccgcgcgac gatgatcgct ggccggaccg
     4681 gctcgtcgtc gcgctcggcc
//
LOCUS       DATAIW010000261         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_245075, whole genome shotgun sequence.
ACCESSION   DATAIW010000261 DATAIW010000000
VERSION     DATAIW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245075"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM48_08250"
     CDS             <1..318
                     /locus_tag="VHM48_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2755439.1"
                     /translation="GRSDGGPIVVVKRGAAGALACRANEAVVRVSAMPVDPIDTTGAG
                     DSFDAGFLRAWLDGRSIPDSLELAAACGALSTGSVGGVDGQPTFEEATAAVRSWRAAR
                     GRP"
     gene            318..1292
                     /locus_tag="VHM48_08255"
     CDS             318..1292
                     /locus_tag="VHM48_08255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX2755440.1"
                     /translation="MAQARIVFVAANPSIDRLYEVDRLTVGAIHRPVALVAVPGGKGL
                     NAARAAARLGGRVTALGILAGRSGDWIEEAISGRRIDARWVRDGGETRTCVSILDRSS
                     GAMTEIYERGEPVDPAAWEALERLVADELARGDVAALAVSGSLPVGAPPDGFGRIAGL
                     ARTADPGARPVAVLADTYGPALAAVLAERPALVKINAEEAAEATGIVVTDASSAETAA
                     AVLLDRGAAGVVVTLGPAGAVVVTAVSSNRLVPDGVRGAYPVGSGDAFLGGLAVGLAR
                     GDDALEAARLGLAAGTANALVPGAGELDPLEVDRILRGTPRRTPGPTG"
     gene            complement(1339..2001)
                     /locus_tag="VHM48_08260"
     CDS             complement(1339..2001)
                     /locus_tag="VHM48_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755441.1"
                     /translation="MTRAIRILIVDDHPLLLDGLVATLSADEEIEVVGTAGDAASAVR
                     AAREHQPDLVLLDVAMPGGGIEAARQIGPASPSTRVVMLTSSENEDDLLAAMEAGAKG
                     YVLKGVAGRELRIILKSVHRGEVYVAPGLAYAMIKGLTRPRQHDPLEELTTREREVLE
                     LVAGGLSNAEIGGRLGLAEKTVKHYMTAILGKLEVGSRVEAALLAYKAGMRGGGSGPT
                     DD"
     gene            complement(1998..3485)
                     /locus_tag="VHM48_08265"
     CDS             complement(1998..3485)
                     /locus_tag="VHM48_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2755442.1"
                     /translation="MLRGRATRLKTHAGDLRRRRPATIRRPNGRLRWFDLSGLSLARR
                     YLLLSFVFVVIGGGVVALALGQLIETSAINRTTAVTALYVESFVAPELQTLATSSELS
                     QAEIDSLQRLLTDTPLGDAVVSFRIWSTDGRVLYSPFPELIGQRFDMSGERGEAALGA
                     VIGDISDLSDPENIYERRQWSHLIELYLPVRASGSSKVIAVAEFYQLPNELEAEVNRD
                     RLIAWGLVAGATVLAYLALVRVVRQGSETILNQQDELRQRVDELSTLLDQNARLSDRI
                     RHAAARSTALTELERRRIGSDLHDGPSQSLAFAMLRLDAVEGRLDASGQAGDADLLAV
                     RSAMDEALRDMRVIAAGLRTPELERASPAAIVQRAVAEHERRAGTPAAVDVRGIPDAA
                     PLATKIALHRILSEALSNAARHGGGIGIEVRATEADGDLVVEVKDAGAGFDPRQKPGE
                     GHLGLAGMRERTELLGGRFVLESGAGTGTRVRAFLPLTGPTSDET"
     gene            3706..4200
                     /locus_tag="VHM48_08270"
     CDS             3706..4200
                     /locus_tag="VHM48_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755443.1"
                     /translation="MTRRNLTTTFMVVGAGLLLFGGVTLAREAQTRDDRNNPAIATPT
                     AVATDDNGGATASPELGDDNGGNGADDSASPTASPDDHGGNGTDDGTAATASPDDHGG
                     NRGGSDDAATPRPTTRPTATATASPDDHGGGSGGHGGDDTPSPTATASPDDNGGGGHG
                     GGDN"
BASE COUNT          604 a         1555 c         1579 g          620 t
ORIGIN      
        1 ggacgatcgg acggtggacc gatcgtggtc gtcaagcgcg gtgccgccgg tgccctcgcc
       61 tgtcgtgcca acgaagccgt cgttcgtgtt tcggcgatgc cggtcgatcc gatcgatacg
      121 accggcgccg gcgattcgtt cgatgccggg ttccttcgcg catggctgga cggccgcagc
      181 attccggaca gcctcgagct cgcggccgcg tgcggggcgc tgtcgaccgg cagcgtcggc
      241 ggggtcgacg gccagcccac cttcgaggag gcgaccgcgg cggttcgttc gtggcgagcg
      301 gcgcgcggac gaccgtgatg gcgcaggccc ggatcgtgtt cgtcgcggcc aatccgtcga
      361 tcgaccgcct gtatgaggtc gaccggctga cggtcggcgc gatccatcgg cctgtggcgc
      421 tcgtcgcggt gcccggtggc aaagggctga acgcggctcg tgcggcggcc cggctcggcg
      481 gacgcgtcac tgcgctcggc attctcgccg gtcggagtgg cgactggatc gaggaggcga
      541 tcagcggccg ccgcatcgac gcccgctggg tccgcgacgg cggcgagacg cggacctgcg
      601 tctcgattct cgatcgctcc agcggcgcca tgaccgagat ctacgagcgc ggcgagccgg
      661 tcgacccagc agcgtgggag gcgctcgagc gcctcgtggc cgacgagctc gcacggggcg
      721 acgtcgccgc gctcgccgtg tcggggagcc tgccggtcgg cgctcccccg gacggatttg
      781 gccggatcgc gggcctcgcc cggacggccg atcccggggc ccgacccgtc gccgtgctgg
      841 ccgacaccta cggtccggcg ctcgcggccg tcctggccga gcgacccgcg ctcgtcaaga
      901 tcaacgccga ggaggcggcc gaggccaccg ggatcgtcgt caccgacgcg tcgtccgccg
      961 agaccgccgc ggcggtcctg ctcgatcgtg gcgctgccgg tgtcgtcgtc acgctcggcc
     1021 ccgctggggc cgtggtcgtg acggccgtca gttcgaaccg gctggtcccg gacggggtcc
     1081 gcggcgcata tccggtcggg agcggcgacg cattcctcgg cggcctggcg gtcgggttgg
     1141 cgcgcggcga cgatgcgctc gaggcagccc ggcttgggct tgcggccgga acggcgaatg
     1201 cgctcgttcc tggcgccggc gagctcgatc cgctggaggt cgaccggatc ctccgaggga
     1261 ctccacggcg gaccccggga ccgaccggct gacctgcgtc gacacgatcc tggagatcga
     1321 gcgatgcacg ggactcgatc agtcgtcggt gggaccgctc ccgccgccgc gcatgccggc
     1381 cttgtaggcg agcagggccg cctcgacccg gctgccgacc tccagcttgc cgaggatggc
     1441 ggtcatgtag tgcttcacgg tcttctcggc aagcccgagg cgaccgccga tctccgcgtt
     1501 cgagaggccg ccggcgacga gttcgaggac ctcgcgctcg cgagtcgtca gctcctcgag
     1561 cggatcgtgt tggcgaggcc tggtcaggcc cttgatcatg gcgtaggcca atcccggggc
     1621 gacatagacc tcgccgcgat ggacggactt gaggatgatc cggagctcgc gtccggcgac
     1681 gcccttgagc acgtagccct tggcgccggc ttccatggcg gccagcaggt catcctcgtt
     1741 ctccgagctg gtcagcatca ccacccgggt cgacggcgag gccgggccga tctgcctggc
     1801 ggcctcgatg cccccgcccg gcatggcaac gtccagcagg acgagatcgg gctggtgttc
     1861 ccgggcggca cggaccgccg acgcggcgtc cccggcggtg ccgacgacct cgatctcctc
     1921 gtcggccgag agggtcgcga ccagcccgtc gaggaggagc gggtggtcgt cgacgatcag
     1981 gatgcggatg gcccgggtca agtttcgtcg ctcgtcggtc cggtcagtgg caggaatgcc
     2041 cggacacggg tgccggtccc tgcccctgac tccagcacga accggccgcc cagcagctcc
     2101 gtccgctcgc gcatgccggc aaggccaagg tggccctcgc ctggcttctg cctgggatca
     2161 aaccccgcgc ccgcgtcctt cacctcgacg accaggtcgc cgtccgcctc ggtcgccctg
     2221 acctcgattc cgatgccgcc cccgtggcgg gcggcgttcg acagggcctc ggacaggatc
     2281 cggtgcaggg cgatcttcgt cgcgaggggt gcggcgtcgg ggatcccgcg gacatcgacg
     2341 gcagccggcg tgcccgcccg gcgctcgtgc tcggcaacag cgcgctggac gatcgcggcc
     2401 gggctcgcac gctccagctc gggggttcgc agtccggccg cgatcacccg catgtcccgg
     2461 agggcctcat ccatggcgct gcgaacggcc agcagatcgg catcgccggc ctggccggag
     2521 gcatcgagac gtccctcgac ggcatccagg cgaagcatgg cgaaggcgag gctctgactc
     2581 ggcccgtcgt ggaggtcgga gccgatccgg cgacgctcca gctcggtcag ggcggtggac
     2641 cgcgcggcgg catggcggat ccggtcgctc agccgtgcgt tctggtcgag cagggtcgac
     2701 agctcgtcga cacgctggcg gagttcgtcc tgctggttga ggatcgtctc gctcccctgg
     2761 cgaaccacgc ggacgagggc caggtacgcc aggaccgtgg cgcccgcgac caggccccat
     2821 gcgatcaggc gatcgcggtt cacctcggcc tcgagctcgt tgggcagctg gtagaactcg
     2881 gcaaccgcga tgaccttcga cgagccgctg gcgcggaccg gcaggtagag ctcgatcagg
     2941 tggctccact gtcgtcgctc gtagatgttc tcggggtcgc tcaggtcgct gatgtcgccg
     3001 atgaccgcgc ccagcgccgc ctcgccccgc tctccgctca tgtcgaagcg ctggccgatc
     3061 agctcgggaa acgggctgta caggacacgc ccgtccgtcg accagatccg gaacgacacc
     3121 acggcgtcgc cgagcggcgt gtcggtgagc aggcgctgga gcgagtcgat ctccgcctgg
     3181 ctgagctcgg agctggtcgc gagcgtctgc agctccggcg cgacgaagct ctccacgtag
     3241 agggcggtca ccgccgtcgt ccggttgatg gcgctcgtct cgatcagctg gcccagcgcc
     3301 agtgcgacga ccccgccgcc gataaccacg aagacgaagc tcagcaggag gtagcggcgg
     3361 gcgagggaca ggcccgagag gtcgaaccag cgcaggcgcc cgttcggtcg tcggatcgtc
     3421 gccggccgtc gtcgtcgcag atcgcctgcg tgcgtcttca gccgggtcgc tcgccctcgg
     3481 agcacgtgtc ctccagttcc gttgcgcctg gccattgtag gagcgggtcc tcggcccgag
     3541 atcggaccgg ggtcccgccg ggcatcagac caaggtccgg tcggtttcgg aaccgcggcc
     3601 cgtggtggcg gggctggccg gacggcaatg ttcgggtcag caaggaccaa gcgggttcga
     3661 ccggacccga cggccagatg tcatcccaga actgaggcat acgacatgac tcgacggaac
     3721 ctgacgacga ccttcatggt tgtcggagcc ggactcctcc tcttcggcgg agtcaccctg
     3781 gcccgcgagg cccagacccg cgacgatcgc aacaacccgg cgatcgccac cccgacggcc
     3841 gtcgcgaccg acgacaacgg cggcgcgacc gcgtcgcctg agctcggcga cgacaacggc
     3901 ggcaacggcg ccgacgacag cgccagcccg accgcatcgc ccgacgacca cggcggcaac
     3961 gggaccgacg acgggaccgc cgcaacggcc tcgcccgacg accacggcgg caatcgcggc
     4021 ggcagcgacg acgcggccac cccccggccg acgacccggc cgacggccac cgcgacggcc
     4081 tcgcccgacg accacggggg cggcagcggc ggccacggtg gcgacgacac cccgagcccg
     4141 acggccacgg cctcgcccga cgacaacggc ggcggcggcc acggcggcgg cgacaactag
     4201 agcgaccggc ctcggccaac gcggccacga acaaacagag aacgcgggct ttcgggcccg
     4261 cgttctctgt tctggcgagg gtcggccagg ctctacttct tcgcgatccg gtggcttgcc
     4321 gtggcgctgt tcggcaggta gttgccgtcg cccatgtg
//
LOCUS       DATAIW010000262         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_215219, whole genome shotgun sequence.
ACCESSION   DATAIW010000262 DATAIW010000000
VERSION     DATAIW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215219"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1436)
                     /locus_tag="VHM48_08275"
     CDS             complement(231..1436)
                     /locus_tag="VHM48_08275"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755444.1"
                     /translation="MSDDPLPIRADDPTAHLSGHLPPPSPGRRWRQFDRLVVVVFCAG
                     LLVPALLMAVRIRPAAIENRALLTAPPITLGSLIDSSWYAAVDRFIADNIAVRPLAVR
                     LRGEVYWRTGGTGNPTVVRGTGSWLFTLEEIQASCDLGAADIAAALDRTDAAFTAAGQ
                     DFRFLVAPDKHVVYPEKVDPGMAYGPSCTDDRRAAMQAALDARPGVAVDGWAAVLAER
                     AAHPDGPLLYYTEDSHWTTSGAVPAIRALIQSLGPDLWRDEDVVRGGSKRVAMELARQ
                     MGLSRAETVPFVTIRPTVRIVRTPIDLPVATNNARAVYRITASGDRPLVPGRTVIVYD
                     SFFGLNMAAVSPFFAESIWIHQGDLLNHPEIAQLVGPADRVILERVERGLYFTKIDDL
                     LRPLVRTGG"
     gene            complement(1445..2869)
                     /locus_tag="VHM48_08280"
     CDS             complement(1445..2869)
                     /locus_tag="VHM48_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX2755445.1"
                     /translation="MVFSSVTFLFFFLPAVLGAYYVVPRRGRNAVLVLASLAFYTWGA
                     GSIVFALIGSVAANFVIGLRLEQAHDRGDATRARRWLAVGVVINLALLGWFKYANFGV
                     ATWNGAVEAFGAPGIPWAAIVLPIGISFYTFHALSYLMDIHRGSARHLESPIDFVLYI
                     TFFPQLIAGPIVRFHEIRDQLVERQETVDAFAAGIYRFSLGLGKKVLIADAVAPVANA
                     AFGTPTGELTMFPAALGVIAYAVQLYFDFSGYSDMALGLALMFGIRLPENFDRPYAAR
                     SVTEFWRRWHMSLSRWFRDYLYVPLGGNRGSARATYRNLVIVFLVTGLWHGANWTFVL
                     WGAYHGILLLLERISGAGRGAGIRPAGWQIVTVVLVVLGWVLFRSPTLDYAAGYYAAL
                     LRPGLILPLDVRVALDPLATACLVFGAGSVLLPRTWVTGVRLENPLPRAHRVLRLVVI
                     GVVLPAALVFVISGTFSPFLYFQF"
     gene            3008..4336
                     /locus_tag="VHM48_08285"
     CDS             3008..4336
                     /locus_tag="VHM48_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX2755446.1"
                     /translation="MGHDARVAIIGLGYVGLPLAIAFEEAGLEVVGVDASEHRVAELR
                     AGTSPIDDISDKRLGAALAKGLRVAAPAEARLAEADAIFVCVPTPITDSKDPDLGPVL
                     SAAEMIATGIRAGQLIVLQSTTFPGTTTGPFREVLERSGLVAGRDFDLAFAPERVNPG
                     DPASASRDVPRLVGATTPDATKRAAVLLHHVNANVVELTSPDAAEMAKLLENVFRNVN
                     IAFVNQLALLCERMGLDVWEVIDAAATKPFGFMRFTPGPGVGGHCIPVDPYYLSWRAR
                     QFDFVDRFVELAGDINLAMPRHVIDLVAEALNERGRAVRGAKVGVIGVAFKPDVRDAR
                     NSPAADVLAGLVGRGAAISYHDPHVPGFVDADGARFESTSLDELLAASDVIVVLAAHR
                     AVDWKRVYGAADLIVDTVNNSRDRAVRPRQVLRLGAGWAVAPASSAVIPA"
     gene            4333..5442
                     /gene="wecB"
                     /locus_tag="VHM48_08290"
     CDS             4333..5442
                     /gene="wecB"
                     /locus_tag="VHM48_08290"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2755447.1"
                     /translation="MTDRVVHVVGARPNFMKAGPVVEALGRRGIEQLVIHTGQHYDRT
                     MSDIFFSELGLPEPDVNLGVGSGSQATQTAALLTALEPALLELDPALTIVYGDINSTL
                     ATSLVASKLHLPTAHVEAGLRSFDMTMPEEVNRRVTDILADLCFATSPEAIDNLGREG
                     VAAERIHFVGNPMIDTLLANLERFDVAAARAEYDLGGPYAVATLHRPANVDTPEAAAR
                     LVAMLDAVMAQLPVVLPLHPRGRAMLAAAGLVDGPRLRLIEPLGYIAFLSLVRGAAVV
                     VTDSGGIQEETTILGVPCLTVRPNTERPITISHGTNRLVEPDEVGPWVARILAAPPGA
                     PVLAATEPPPLWDGHAGDRIAAIVIGWLAARTPAS"
     gene            5412..>6186
                     /locus_tag="VHM48_08295"
     CDS             5412..>6186
                     /locus_tag="VHM48_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2755448.1"
                     /translation="MAGRPDARVVIRREILRHARLTRALRRIRGAIRHAGRRTGGTSA
                     GPRRYPTDFQVHPAPPAIAGDWPEPPAIDSPFPALHEPAPPSDPIRHDLALFEQLNAE
                     YAAKPVAPIAPRYDSASLSQRSRSRLTAVHDRIGLAGRTVLEVGCGAGYEVWFLAHHL
                     GCDAWGIDISPRRAWPILAGGRVHLVEGDIATRGSLPANTFDRVISFTVWEHVTHPLE
                     AITELERVMKPGGLAWVRANLYRGPTASHRTRDIRFPFPH"
BASE COUNT          954 a         2248 c         2130 g          854 t
ORIGIN      
        1 gccccggccc cgtccatggc ccgcgggcgc ccgccggaat cagccagccc tgcggtttcg
       61 aatagccgaa tacccgtgac gcttgggtgg tccgcgtggg actccgcatc gctcctgccc
      121 aggcccgtga ttcgagattc gaatcattcg gctattcgca cgggctgcgg ggaccgatct
      181 ggcgcgccgc tccgtgccgc ccagcgcgcc gccggcgccc cggaacgatg tcagccgccg
      241 gtccggacca gcggccgcag caggtcgtcg atcttcgtga agtacaggcc gcgttcgacc
      301 cgttcgagga tcacccggtc ggcgggaccc acgagctgcg cgatctccgg gtggttgagc
      361 aggtcgcctt ggtggatcca gatcgactcg gcgaagaacg gcgagacggc ggccatgttc
      421 agcccgaaga agctgtcgta gacgatcacg gtccggcccg gcacgagcgg acgatcgccg
      481 ctcgcggtga tccgatagac cgccctcgcg ttgttcgttg cgaccggcag atcgatcggc
      541 gtgcggacga tccggacggt cgggcggatc gtcacgaacg ggaccgtctc ggcccggctc
      601 aggcccatct ggcgggcgag ctccatggca actcgtttcg acccgccgcg cacgacatcc
      661 tcgtcgcgcc acaggtcggg tccgagcgac tggatcaacg cccggatcgc cgggaccgcg
      721 ccgctggtcg tccagtgcga gtcctcggtg taatagagca gcggcccgtc cggatgagcc
      781 gcccgctcgg cgaggacggc tgcccagccg tcgacggcaa cgcctggacg tgcatcgagc
      841 gcggcctgca tcgccgcccg ccggtcgtcg gtgcacgagg ggccgtaggc catccccgga
      901 tcgaccttct ccgggtacac cacgtgcttg tcgggggcga cgaggaagcg gaagtcctgg
      961 ccggcagcgg tgaatgcggc atcggtccga tcgagcgcgg cggcgatgtc ggctgccccg
     1021 aggtcgcagc tcgcctggat ctcctcgagc gtgaacagcc acgatccggt tccacggacg
     1081 acggtcggat tgcccgtccc tccggttcgc cagtagacct cgccgcgaag ccggaccgcg
     1141 agcgggcgga cggcgatgtt gtcggcgatg aagcgatcga ccgcggcgta ccagctcgaa
     1201 tcgatcaggc tcccgagggt gatggggggc gcggtcagga gcgctcgatt ctcgatcgct
     1261 gccggccgga tccgcacggc catgaggagt gcgggaacga gcaggccggc gcagaagacg
     1321 acgaccacga gtcggtcgaa ctggcgccag cgccggcccg ggctcggcgg cggcaggtgc
     1381 ccggacaggt gggcggtcgg atcatcggcg cggatcggca ggggatcgtc gctcatgctc
     1441 gctctcagaa ctggaagtac aggaacgggc tgaacgtgcc cgagatgacg aagacgagcg
     1501 ccgccggcag gaccaccccg atcaccacga gccgcagcac ccgatgggcc cgcgggagcg
     1561 ggttctccag gcggacgccc gtcacccatg tccgcggcag cagcaccgag cccgcgccga
     1621 agacgaggca ggcagtcgcg agcggatcga gggcgacgcg gacgtccagc ggcaggatga
     1681 gacccggccg caggagcgcc gcgtagtagc ccgccgcgta gtcgagggtc ggcgaccgga
     1741 acagcaccca gcccagcacg accaggacga ccgtgacgat ctgccagccg gccggtcgga
     1801 tgcccgcgcc gcgcccggct ccgctgatcc gctcgaggag cagcaggatg ccgtggtacg
     1861 cgccccacag cacgaacgtc cagttcgccc cgtgccagag cccggtcacg agaaagacga
     1921 tcacgaggtt gcggtacgtc gcccgggccg acccgcggtt gccgccgagt ggcacgtaca
     1981 ggtagtcgcg gaaccagcgc gacagcgaca tgtgccagcg ccgccagaat tcggtcacgc
     2041 tccgcgcggc atacggtcgg tcgaagttct cgggcaggcg gattccgaac atgagcgcca
     2101 gcccgagggc catgtccgaa tacccgctga agtcgaagta cagctggacc gcataggcga
     2161 tgacgccgag cgccgccggg aacatcgtca gctcgcccgt cggcgtcccg aatgcggcgt
     2221 tggccaccgg cgccaccgca tcggcgatca ggaccttctt gccgagcccg agcgagaacc
     2281 ggtagatacc ggcggcgaac gcgtcgaccg tctcctgccg ctcgacgagc tggtcccgga
     2341 tctcgtggaa ccggacgatc ggcccggcga tcagttgcgg gaagaacgtg atgtagagga
     2401 cgaagtcgat cggagactcg agatggcggg cgctgccgcg atggatgtcc atgaggtagc
     2461 tgagggcgtg gaacgtgtag aacgagatac cgatcgggag gacgatcgcg gcccacggga
     2521 tccccggggc gccgaacgcc tcgaccgcgc cgttccaggt cgcgaccccg aagttggcgt
     2581 acttgaacca gcccagcagc gccaggttga tgacgacgcc gacggccagc cagcgcctcg
     2641 cccgggtcgc gtcgccacga tcgtgagcct gctcgagccg gaggccgatg acgaagttgg
     2701 ccgcgaccga accgatcagg gcgaagacga tcgaaccggc gccccatgtg tagaacgcga
     2761 ggctggcgag gacgaggacc gcgttccgtc cgcgacgggg gacgacgtaa taggcgccca
     2821 ggacggcggg caggaagaag aacaggaacg tgaccgagga gaagaccatg cgcgggccgg
     2881 agaatacacc gggtctcgcg ccccggccgg gccggccggc catcgccgtc cgctagactt
     2941 cggcggtccc acggcgcgcc ggcaacacca tcgctcggcc gccgccctgg gccgctggag
     3001 gatcggcatg ggtcacgacg cgcgcgtcgc catcatcggg ctcggctacg tgggtctgcc
     3061 cctcgcgatc gcgttcgagg aggcgggtct cgaggtcgtc ggcgtcgatg cgagcgaaca
     3121 ccgggtggcc gagttgcgcg ccggaacatc gccgatcgac gacatcagtg acaagcgcct
     3181 cggggcggcc ctggccaagg gcctccgggt tgccgcgccg gcggaggctc gcctggccga
     3241 ggccgatgcg atcttcgtct gcgtccccac gccgatcacc gactcgaagg accccgacct
     3301 cggtccggtc ctgagcgcgg ccgagatgat cgccaccggg atccgggcag gccagctcat
     3361 cgtcctccag tcgaccacgt tccccggcac gacgaccggt cccttccgcg aggtcctcga
     3421 gcgcagcggc ctggtcgcgg gccgcgactt cgatctcgcc ttcgcccccg agcgggtcaa
     3481 tccgggcgat ccggccagcg cgtcgcggga cgtgccgcgc ctcgtcggag cgaccacgcc
     3541 ggatgcgacg aaacgagcag ccgtccttct ccaccacgtc aacgccaatg tcgtcgagct
     3601 cacctcaccc gacgcggccg agatggccaa gctcctcgag aacgtcttcc gcaacgtcaa
     3661 catcgcgttc gtgaaccagc tcgccctgct ctgcgagcgg atggggctcg acgtctggga
     3721 ggtcatcgac gcggcggcca ccaagccctt cgggttcatg cgcttcacgc ccgggccggg
     3781 cgtcggcggc cattgcatcc cggtcgatcc ctactacctc tcgtggcggg cccgccagtt
     3841 cgacttcgtc gatcgcttcg tcgagctcgc cggcgacatc aacctcgcga tgccgcggca
     3901 cgtcatcgac ctcgtggcgg aggcgctcaa cgagcgcggc cgggccgtcc gcggcgcgaa
     3961 ggtcggcgtc atcggcgtcg ccttcaagcc cgatgtgcgc gatgcccgga actcgcccgc
     4021 ggccgacgtg ctggccggcc tggtcgggcg cggggcggcg atctcctatc acgatccgca
     4081 cgtcccgggc ttcgtcgacg cggacggagc gcggttcgaa tcgacctcgc tcgatgagct
     4141 cctcgccgcc tccgacgtga tcgtcgtcct cgcagcccat cgggccgtcg actggaaacg
     4201 ggtctacgga gcggccgacc tgatcgtcga caccgtgaac aactcgcggg atcgggcggt
     4261 ccggccgcgc caggtcctgc ggctcggggc gggctgggcg gtcgccccgg cgtcgagcgc
     4321 ggtcattccc gcgtgaccga ccgggtcgtc catgtcgtcg gcgcccggcc gaacttcatg
     4381 aaggccggcc ccgtcgtcga ggccctcgga cggcgaggca tcgagcagct cgtcatccac
     4441 accggccagc actacgaccg gacgatgtcc gacatcttct tcagcgagct cggcctgccc
     4501 gagccggacg tcaacctcgg cgtcgggtcg ggcagccagg ccacccagac cgcggccctg
     4561 ctgacggccc tcgagcccgc cctgctcgag ctcgatccgg cactgaccat cgtgtacggc
     4621 gacatcaact cgacgctcgc gacctcgctc gtcgccagca agctccacct gccgacggcc
     4681 catgtggagg cgggcctgcg gagctttgac atgaccatgc ccgaggaggt caaccggcgg
     4741 gtcaccgaca tcctggccga cctctgcttc gcgacgagcc ccgaggcgat cgacaacctc
     4801 gggcgcgagg gcgtggccgc cgagcggatc cacttcgtcg gcaacccgat gatcgacacg
     4861 ctcctcgcga acctcgagcg gttcgacgtg gcggccgccc gagccgaata cgatctcggt
     4921 ggtccgtacg ccgtcgcgac gctccaccgg ccggccaacg tcgacacgcc cgaggcggcg
     4981 gcccgcctcg tcgccatgct cgatgcggtg atggcccagc tgcccgtcgt cctgcccctc
     5041 catccccgcg gccgggcgat gctcgccgcc gcgggcctgg tcgacggccc gcgtctccgc
     5101 ctgatcgagc cgctcggcta catcgcgttc ctgtcgctgg tccgtggcgc ggcagtcgtg
     5161 gtcaccgact cgggcgggat ccaggaggag acgacgatcc tcggcgtgcc ctgcctgacg
     5221 gtccggccga acaccgagcg cccgatcacg atcagccacg gaacgaaccg cctggtcgag
     5281 cccgacgagg tcggcccgtg ggtcgctcgg atcctggccg caccgccggg cgccccggtc
     5341 ctcgccgcga ccgagccgcc gccgctctgg gacggccacg cgggcgaccg catcgccgcc
     5401 atcgtgatcg gttggctggc cgcccggacg cccgcgtcgt gatccgccgc gagatcctgc
     5461 gacacgcgcg cctcacgcgg gcgctgcgcc ggatccgggg cgcaattcgc cacgctggcc
     5521 ggcgaaccgg gggaacgtcg gctggtccac gccgctatcc gaccgacttc caggttcatc
     5581 cagcgccacc ggcgatcgcc ggcgattggc ccgagccgcc ggcgatcgac tcaccgttcc
     5641 cggccctgca cgaaccggcg ccgccctcgg atccgatccg ccacgacctt gcgctcttcg
     5701 aacagctcaa cgccgaatat gccgcgaagc cggtcgcgcc gatcgcccct cgctacgact
     5761 cggcctccct gtcccagcga tcgcgcagcc gcctcacggc cgtgcacgac cggattggtc
     5821 tcgccggcag aaccgtcctc gaggtcggtt gcggggcggg ctacgaggtg tggttcctcg
     5881 cccaccatct cggctgtgac gcgtggggca tcgacatctc gccgcgccga gcctggccga
     5941 tcctggcggg cggacgcgtc cacctcgtcg agggcgacat cgccacccgc ggcagcctgc
     6001 cggcgaacac gttcgatcgg gtcatctcgt tcaccgtctg ggagcacgtc acccaccccc
     6061 tcgaggcgat caccgagctc gagcgggtga tgaagccggg tggcctcgcc tgggtccggg
     6121 cgaacctcta ccgcgggccg accgcctcgc atcgcacccg cgacatccgg ttcccgttcc
     6181 cgcatc
//
LOCUS       DATAIW010000263        14058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_45581, whole genome shotgun sequence.
ACCESSION   DATAIW010000263 DATAIW010000000
VERSION     DATAIW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45581"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VHM48_08300"
     CDS             <1..1257
                     /locus_tag="VHM48_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2755449.1"
                     /translation="IAHILKLMGRRVGMTSTDGIVVDGRLIKKGDMSGPKSAQMVLQN
                     PSVDTAVFEVARGGILREGLGYDRNDVAVVTNVTGDHLGIGGIDSIGQLANVKGVVVE
                     AVPRSGTAVLNADDGLVYRMGRHCAGKVVLFSMATRKGEDGYDRVDGHTARGNAAFIL
                     QPGPEGELIVLHHGPRTMPVLYTHLIPATFGGRARMNVANALAAAAAAWAAGAHLHDI
                     RQGLRTFSTSFFQAPGRLNLLDVGGIRVVIDYCHNVDGMRHLADFVERMMTEPQSKAG
                     RIGPSTNGRSRSGRAIGVIGIPGDRRDEDQVEFGSIAARAFDEIIVREDKNLRGRAPG
                     VSAGHVIDGVRASRAAGARTLKADKMLDEMTAVRAALRRAAPGDLVVCCVDDAIGVYR
                     EAMAASGHARGATAFADPGEFEVPEG"
     gene            1257..2342
                     /locus_tag="VHM48_08305"
     CDS             1257..2342
                     /locus_tag="VHM48_08305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2755450.1"
                     /translation="MSRLAGLDREAFRQWLAESDFSGVVHVAGDDDEEPLAIAMGQAD
                     RVAGLPNHAGTRFGIASTTKLFTGLTVARLVDRGVVRYEDRLVDLLAEDLRPLDLDPR
                     VMIHQLLSHTSGVGDYADEYDGPPYETIWESIPPTMIRGPRDMVPLLRDLPRTGDPGE
                     AARYNNGAYVLVGLALEIVSGRSFPDLVRAEVFGPLGMTTSGFWAFDGLEPDLADGYL
                     PPDPEAAPGTLATTWRTNIYSLPAIGLPDGGAQATATDLVRALDGLTGRGAVGAEFLT
                     PTTRERMIGPHAVSPVEKAGYGLGVIHGGEGPSARIGHAGEDPGFSSRCWAYRASGER
                     VVVQSNVTEGAWHPFRRLDELLAAVDH"
     gene            complement(2425..4239)
                     /locus_tag="VHM48_08310"
     CDS             complement(2425..4239)
                     /locus_tag="VHM48_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2755451.1"
                     /translation="MRLIEIRLLEGPNAYRLEPVVKVEVAIGRRRTWYGQRSPGRHAL
                     VQLGANVPARDWPLPIAELVGWLQRLRVDHGEGRGGVHVHRSSDPGHWIVTFGWSGAE
                     RARTIAEAALALSDRDVSSVRRTHLTGSQERLVARWTDRIADSRSTPPSWIRNADRRI
                     PIISITGTNGKSTVTRLITHILKVAGRRVGTTTSDGILVDERLVEPGDWTGPGGAWQV
                     LERSDIDVAVLETARGGLVLRGMGYESNDASVVTNVTSDHLDLQGIHTLPELAEVKAT
                     VVRATREGGWAVLNADDPLAAAMTRRIRANVAVFSLGVGSGGSAVVRRTVAGGGRGYI
                     LRDGWLIEIDGSRGDGGYAKAGDGAVSGSPVEHRIIEVDRVPITLGGLARHNVANALA
                     AAGAVRGIGVTLAQLRDGLADFQPSSERSPGRLNLFRLGSRIVIIDFAHNEAGVNAVL
                     DVAEGIAGGAAGRMAPVTVIIGTAGDRPDDTLRGIGRIAGKRAQRVAIKETLKYLRGR
                     SRESIVGEILGGVAAAGRTPADVPVYESETEALRAELARNGEGAARPDAPRVIVLMCH
                     EERTEVFALLTELGAHPIDVASELTTLIPRLQGRPRRA"
     gene            4330..5586
                     /locus_tag="VHM48_08315"
     CDS             4330..5586
                     /locus_tag="VHM48_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2755452.1"
                     /translation="MDTLTAPGGTVADAELTAIETAIRAAEPAYLVDLERLVNTDCGS
                     YTPAGVDEIGRFVAAFMTAHGATVEVRPDPAGTRGGTVIGTWDGPRGTAGGPRVLLIG
                     HMDTVFDPGTAAARPFAIDADGIARGPGVTDMKSGLLAGLYALQALSAVGPLPFERLT
                     YVANPDEEIGSPSSTPYIREIAAESDVCLVLECARANGDIVSSRKGILDARITVHGRA
                     AHAGVEPEKGRSAILAAADLVTRLHALNGRWDGVTVNVGVIGGGTRPNVVAEACSFEV
                     DLRAVRRSDLEAAEAEIRTMLRTLVVPDVTAELEPMARWWPMEKLERSGRLVEHARAI
                     AGRLGFEVRDAATGGASDANTTSGMGVASIDGLGPIGGMDHSPAEYLEVASIVPRTAL
                     LAGLLLAIGRDPVVAEWRAAGPAATS"
     gene            5583..5975
                     /locus_tag="VHM48_08320"
     CDS             5583..5975
                     /locus_tag="VHM48_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX2755453.1"
                     /translation="MSGERRRVSSGSPYEARYGYSRAVAVGDSCWVAGTTDAGPDGIS
                     RNPGDAAAQARAAFAIGIAALEEAGFAAADVVRTRMYIVRSGDAAAVAAVNGELFSAI
                     RPASTLVRVAGLIEPSMLVEVELEARRG"
     gene            complement(5988..6317)
                     /locus_tag="VHM48_08325"
     CDS             complement(5988..6317)
                     /locus_tag="VHM48_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX2755454.1"
                     /translation="MSTDLVPLAVLMFAVTYPSRAIGLLNPAIDRLPRRALEYLQLVG
                     PAVLAALAATQVMVAPRDDRWVFVVGLPWLSVLACLVIVAWRRSLFVGLAAAVAIAIA
                     GRAAGLP"
     gene            complement(6314..7177)
                     /locus_tag="VHM48_08330"
     CDS             complement(6314..7177)
                     /locus_tag="VHM48_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX2755455.1"
                     /translation="MRRDLRKDLPALKPDVPAQIDAAALGRARRRLTLDGLGIIVSAT
                     GFGFVFGLTARTTGHFSPIEAMAMSVIAFAGAAQFAAVGYVAAGLSWPAIGLLTFLLN
                     ARHILYSAALAPWFRGRSFVERAVAAHLLTDEAFALSIAHFRRVGRFDAIGYWYGAIV
                     TTLIPWNVATFAGVMLGDAIVNPQRLGIDVISPAAMGGLAIGLISGRRELAAAFGGAA
                     IGVAASLVSSPTVGIIAGGLFGPLVGLLIPPAQAREVATIGSPESAGRYGVPRTPGSA
                     TSDARHEDRSR"
     gene            complement(7141..7419)
                     /locus_tag="VHM48_08335"
     CDS             complement(7141..7419)
                     /locus_tag="VHM48_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755456.1"
                     /translation="MSVTSNVGQSYPYTSETEADRATRIGALVAERDGLSDQLEAETT
                     PLDSNERWWVWKCPTPGCPGLLHVAGYAAEKHALFVVCDGTCGKTFLR"
     gene            complement(7582..9249)
                     /gene="ggt"
                     /locus_tag="VHM48_08340"
     CDS             complement(7582..9249)
                     /gene="ggt"
                     /locus_tag="VHM48_08340"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2755457.1"
                     /translation="MPDRPLVPMPLVRGAHGAVVAPHHLATAAGLAILRAGGHAVDAA
                     IATNAVLGVVLPSGCGLGGDAFWLIWDEAAGRQTALNGSGRAPARADAAALRAGGLAT
                     LPRRGPLAITVPGAVRSWDDAHRRHGRLSRAEVLGPAIELAWDGFPAWDGFISAVEGT
                     LPAVVAALGPRSGFERVYRPHGRAWRPGERVKLPALAATLERLAEVGFDDFYDGEIGE
                     RQARGLAEVGSAISVDDLRSHTSTWTEPIATDYRGVRVTTHPPNSSGIVALELLNILE
                     TFEPPASSMFGPHGVEDARWVHLGVEAAKLAMADRDRWLTDPAFLDIPLDQLTSKAYG
                     EELAARIDPARASRPPASTNPRGGGTIYLASVDAEGNAVSLIQSNYMGFGSGVVDADT
                     GIHYQNRGSYFSLDPAHPNVLAPGKRTLHTLLPGMLFRAGERRPWVVTGSMGGDAQPQ
                     IHAQVVSALVDGGLDIATTVGAPRWFVDGDQHFAPPVDIRAERRYPAAVLDALEAMGH
                     PVTRTIPFNGLLGHAHAIELIDGGPATADGSLAAATDPRSAGLPAVW"
     gene            9289..10587
                     /locus_tag="VHM48_08345"
     CDS             9289..10587
                     /locus_tag="VHM48_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755458.1"
                     /translation="MRSLYAVLLGTFTLRFSTGLTGGLFLFYLSHLEKDYGGPRTDET
                     TLAVMVALFYASELVLSTPFGLLSDRLGHHRVMQIGPLFGIIASLVTWLSVNLVVIGG
                     TRLLEGASTAASVPSILGFIAMFTAGNELLRGRASARFEGATLAGIGVGAVAAGVLYD
                     GIPGVWGGLHRDAFLLNAGFYVISLLIYRYGVGEPPGEAEAVGGEHTGWRRYSAVLLS
                     SHVWLLAPTWIAINAALGLWASQSIFQLIKVPDPRFADQALMGGFVGAQIALGLGVAL
                     IVFFAGLVFWGDRFKRFRRTTIIVYGIGGGALSVIGAAIVNHGADLVPVALAAAVLAG
                     VGLFVLAGATPAALGLLADMSEAYPSDRGAIMGLYSVFLAVGQIIGSFVGAEAAKHYG
                     IDGILVATLGLLAIALVPIRRLRPFEHRVGSAAMAGSRAA"
     gene            10669..11514
                     /locus_tag="VHM48_08350"
     CDS             10669..11514
                     /locus_tag="VHM48_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2755459.1"
                     /translation="MARIVFVTDSASDLDPAVATALGIRVVPLVVTFGSDTYRAGVDL
                     STAAFWERMTAPDAPFPKTAAASPGDVKSIYDGAFAEGADAIISVHVAGTLSGAIKSA
                     EIAKSMLPDREIHIVDSTGASMAESILCFMGQELAAGGMAAAEIAEILSDRASDLQIY
                     VSLETLAYLKKGGRISGAQAAIGTLLSVKPIIGVEAGKVEIADKPRTRSKSRQRCIEM
                     ITARPIERVAILHTMAPDVEAFRDEVIRRAGLDASDVTVSIVGPSVGPHLGPGCVGAA
                     VLYQR"
     gene            12011..13276
                     /locus_tag="VHM48_08355"
     CDS             12011..13276
                     /locus_tag="VHM48_08355"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2755460.1"
                     /translation="MTIEQVAASINPWLVAQQQFDLAAERLNLDPGLRRVLREPRREL
                     TVHFPVKMDDGSVQVYTGYRIQHNLSRGPAKGGIRYHQNVSLDEVKALAMWMTWKCAV
                     VGIPYGGGKGGVIVDPKKLSQKELEALTRRYFTEIEVLIGPERDIPAPDVNTNGQIMA
                     WMMDTYSMHAGHTVPGVVTGKPISIGGSEGRNEATARGTVFCIVEAARHLGIELNKAK
                     VSVQGFGNAGSIAATLIVEEGATVVAVSDSTGGIHNPNGLDIKRVINWKKEHGTVQGF
                     PGATDISNEAVLEVECDILIPAALENQITNHNVANIKARLIAEAANGPTTPDADQAMF
                     KAGKFMIPDILCNAGGVTVSYFEWVQDLNRDHWSESVVNAKLKEIMVKAFSETLAIAR
                     REQCDMRTAAYLLAVQRVADATALRGLYP"
     gene            13603..>14058
                     /locus_tag="VHM48_08360"
     CDS             13603..>14058
                     /locus_tag="VHM48_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755461.1"
                     /translation="MLDHEEKFLFKEEILHLANAVAEPGTLDNVEDLLTDVITSPRFD
                     SEVFTLERSLQVMLGPRAGTTLVGLLTVAPEVFEEVADVNIPSEVHERLVAWRARFGL
                     AIDNALNFLNNPDGIQGHNFATQVAHVEERRVLQGRLTLVRYNNEPQFFQ"
BASE COUNT         2043 a         4980 c         5015 g         2020 t
ORIGIN      
        1 atcgcccaca tcctcaagct catgggccga cgggtcggga tgacctcgac ggacggcatc
       61 gtggtggacg gccggctgat caagaaaggc gacatgtccg ggccgaagtc ggcccagatg
      121 gtcctccaga acccgtccgt ggacaccgcc gtcttcgagg tggcgcgcgg cggcatcctc
      181 cgcgagggcc tcggctacga ccgcaacgac gtcgcggtcg tcacgaacgt caccggcgac
      241 catctcggga tcggcgggat cgattcgatc ggccagctcg cgaacgtcaa gggggtcgtc
      301 gtcgaggccg tgccgcggtc gggaacggcg gtcctcaacg cggacgacgg ccttgtctac
      361 cggatgggcc gccactgcgc cggcaaggtc gtcctgttca gcatggcgac ccggaagggc
      421 gaggacggct acgaccgcgt cgacggtcac acggcccgcg gcaacgccgc gttcatcctc
      481 cagccaggtc cggagggcga gctgatcgtc ctccaccacg ggccgcggac gatgccggtc
      541 ctgtacaccc acctgatccc cgcaaccttc ggcgggcggg cccggatgaa cgtcgccaac
      601 gctctcgcgg cggccgcggc cgcgtgggcc gcgggcgccc acctccatga cattcgccag
      661 ggcctccgga cgttctcgac ctcgttcttc caggcgccgg gccggctcaa cctgctcgat
      721 gtcggcggga tccgggtcgt catcgactat tgccacaacg tcgacgggat gcgtcatctg
      781 gccgacttcg tcgagcggat gatgaccgag ccgcagtcga aggcgggtcg gatcgggccg
      841 tcgaccaacg gtcgctcgcg gagcggtcgg gcgatcggcg tgatcgggat cccgggcgat
      901 cggcgtgacg aggaccaggt cgagttcggg tccatcgctg cccgggcctt cgacgagatc
      961 atcgttcgcg aggacaagaa cctccgcggc cgggcgccgg gcgtatcggc cggccacgtg
     1021 atcgacgggg tgcgtgcgtc gcgggccgct ggagcgcgca cgctcaaggc cgacaagatg
     1081 ctcgacgaga tgaccgcggt ccgggcggcg cttcgccggg cggccccggg cgacctcgtc
     1141 gtctgctgcg tcgacgacgc gatcggggtg tatcgcgagg cgatggccgc ctcgggtcat
     1201 gcccgcggcg cgacggcgtt cgcggatccc ggcgagttcg aggtgcccga gggctgatga
     1261 gccggctcgc cggactcgat cgcgaggcgt tccggcagtg gctcgcggag tcggacttct
     1321 ccggcgtcgt ccacgtcgcc ggcgacgatg acgaggagcc cctggcgatc gcgatgggcc
     1381 aggccgaccg ggtggccggc ctgccgaacc acgccgggac ccgattcggg atcgcctcga
     1441 cgacgaagct cttcaccggc ctgaccgtgg cgcgtctcgt cgaccgcggc gtcgttcgct
     1501 acgaggaccg cctcgtcgat ctcctcgccg aggacctccg gccgctcgac ctcgacccgc
     1561 gggtgatgat ccaccagctg ctgagccata cctcgggcgt cggcgattac gccgatgagt
     1621 acgacgggcc gccgtacgag acgatctggg agtccatccc gccgacgatg atccggggcc
     1681 cgcgggacat ggttccgctg ctgcgcgacc tgcccaggac cggagatccg ggcgaggccg
     1741 cccggtacaa caacggggcg tacgtcctcg tcggcctcgc cctcgagatc gtgtccggtc
     1801 gatcgttccc cgatctcgtc cgagccgagg tcttcggccc gctcgggatg acgacgagcg
     1861 gattctgggc gttcgacggc ctcgaaccgg acctcgccga cgggtacctg ccgcccgatc
     1921 cggaggcggc gcccgggacc cttgcgacca cctggcggac gaacatctac tcgttgccgg
     1981 cgatcgggct gccggacggc ggcgcgcagg cgacggccac cgacctcgtc cgggcgctcg
     2041 acggactgac cggccgtggc gcggtcggcg ccgagttcct cacgcccacg acccgcgagc
     2101 ggatgatcgg gccgcatgcc gtgagccccg tcgaaaaggc gggctatggg ctcggggtga
     2161 tccatggcgg agagggaccg tccgcccgga tcgggcacgc gggcgaggac ccggggttca
     2221 gctcccgctg ctgggcctac cgggcgtccg gcgaacgggt ggtcgtgcag tcgaacgtga
     2281 ccgagggcgc ctggcatccg ttccgccgcc tcgacgagct gctggccgcc gtcgaccatt
     2341 gactgccacg tgagacgagt atcgtgctac tcgtgagtag cgtgttgttg acgatcctga
     2401 cggctgttga cccgctgctg cccgtcaggc gcgccgcggg cggccctgca agcgcgggat
     2461 cagggtcgtg agctcgctcg cgacgtcgat cgggtgcgcg ccgagctcgg tcaggagggc
     2521 gaatacttct gtgcgctcct cgtggcacat caggacgatg acccgcgggg cgtcgggccg
     2581 cgcggcaccc tcaccgttcc gagccagttc ggcgcggagt gcctcggtct ccgattcgta
     2641 gaccggcacg tccgccgggg ttcggcccgc cgccgcgacg ccgcccagga tctcgccgac
     2701 gatcgactcg cggctccgcc cgcggaggta cttcagggtc tccttgatcg cgacgcgctg
     2761 ggcccgcttg ccggcgatcc tgccgatccc gcggagcgtg tcgtccggcc ggtcgccggc
     2821 cgtgccgatg atcacggtga ccggcgccat tcgcccggcc gccccgccgg cgatgccctc
     2881 tgcgacgtcg agcacggcgt tcacgccggc ctcgttgtgg gcgaagtcga tgatgacgat
     2941 ccgcgacccc aggcggaaca ggttcagtcg accgggcgaa cgctccgatg agggctggaa
     3001 gtcggccagg ccgtcgcgca gctgagccag cgtcaccccg atgccgcgaa cggctccggc
     3061 ggcagccagt gcgttggcga cgttgtggcg ggcaagcccg ccgagggtga tcgggacccg
     3121 gtcgacctcg atgatccggt gctcgaccgg cgagcccgaa actgcgccat cgcccgcctt
     3181 ggcgtagcca ccatcgccgc ggctgccgtc gatctcgatc agccagccgt cgcgcaggat
     3241 gtagccgcgc ccgccgccgg cgacggttcg gcggaccacg gccgagccgc ccgacccgac
     3301 gccgagggag aacaccgcga cattcgcccg gatccgccgg gtcatggccg cggccagcgg
     3361 atcgtcggcg ttgaggacgg cccagccgcc ttcgcgggtc gcccggacga ccgtcgcctt
     3421 gacctcggcg agctcgggca gcgtgtggat gccctggagg tccaggtggt cggacgtgac
     3481 gttggtcacc acgctcgcgt cattcgactc gtagcccatg ccgcgcagga cgaggccgcc
     3541 gcgggcggtc tcgaggacgg cgacgtcgat gtcggatcgt tcgaggacct gccaggcgcc
     3601 gcccggcccc gtccagtccc ccggctcgac caggcgctcg tcgacgagga tgccgtccga
     3661 ggtcgtcgtc ccgacccgcc gaccggccac cttgaggatg tgggtgatca accgggtgac
     3721 cgtgctcttg ccattcgttc cggtgatcga gatgatcggg atccgccggt cggcgttgcg
     3781 gatccagctc ggcggcgtcg accgggaatc cgcgatccgg tcggtccagc gggcgaccag
     3841 ccgctcctgg ctgccggtca ggtgggtccg ccggacggac gacacgtccc ggtcgctcaa
     3901 ggccagcgcc gcctcggcga tcgtccgggc ccgctccgcg cccgaccagc cgaacgtgac
     3961 gatccagtga ccgggatccg acgagcgatg gacgtggacg ccaccgcggc cctcgccatg
     4021 atcgacgcgc aggcgttgga gccagccgac gagctccgcg atcggcagcg gccagtcgcg
     4081 tgccgggacg ttggccccga gctggacgag ggcgtggcgg ccgggactgc gctggccgta
     4141 ccaggtccgt cgccggccga tcgccacctc gaccttgacc accggctcga gccggtaggc
     4201 gttcgggcct tcgagcaggc gaatctcgat gagtcgcacg gttcgggcag gataacgctc
     4261 cggaccgctc acggaacgtc cgtttcggtt gtctggcgga ccacccgacc gcccgcctag
     4321 acttgatcga tggacaccct gaccgcaccc ggcggcaccg tcgcggacgc cgagctgacc
     4381 gccatcgaaa ccgcgatccg agcggccgaa cccgcctacc tcgtcgacct cgagcggctc
     4441 gtgaataccg attgcggctc ctacaccccc gccggcgtcg acgagatcgg tcggttcgtg
     4501 gcggcgttca tgacggccca cggagcgacc gtcgaggttc ggcccgaccc cgccggcacg
     4561 cgcggcggga ccgtcatcgg gacctgggac gggccgcgcg ggaccgcagg tgggccgcgg
     4621 gtcctgctga tcggccacat ggatacggtg ttcgacccgg gcaccgccgc cgctcggccg
     4681 ttcgcgatcg acgccgacgg gatcgcccgc ggacccggcg tgaccgacat gaagtcgggg
     4741 ctcctcgccg gcctgtacgc gctccaggcg ctgtccgccg tgggtccgct gccgttcgag
     4801 cgactgacct atgtcgcgaa cccggacgag gagatcggct caccgagctc gacgccgtac
     4861 atccgcgaga tcgctgccga atcggacgtc tgtcttgtcc tcgaatgcgc ccgggccaac
     4921 ggcgacatcg tctcgtcgcg gaaggggatc ctcgatgccc ggatcaccgt tcacggccgg
     4981 gccgcccatg ccggcgtcga gccggagaaa ggtcgttcgg cgatcctcgc ggccgccgat
     5041 ctcgtgaccc gcctccacgc tctcaacggg cgctgggacg gggtcaccgt caacgtcggg
     5101 gtgatcggcg gcgggacgcg cccgaacgtc gtcgccgagg cgtgctcgtt cgaggtcgac
     5161 ctgcgggcgg tccggcgctc ggacctcgag gcggccgagg cggagatccg gacgatgctc
     5221 cggacgctgg tcgtcccgga cgtcaccgca gagctcgagc cgatggcccg ctggtggccg
     5281 atggagaagc tcgagcggtc ggggcgcctc gtcgagcacg cccgggcgat cgcgggacgg
     5341 ctcgggttcg aggtccgcga tgcggcgacc ggcggagcgt ccgacgcgaa cacgacctcg
     5401 gggatgggcg tcgcctcgat cgacgggctc ggcccgatcg gcgggatgga tcactcgccg
     5461 gcggaatacc tcgaggtcgc ctcgatcgtg ccgcggaccg cgctcctcgc cgggctgctg
     5521 ctcgcgatcg gccgcgaccc ggtcgttgcg gagtggcggg ccgccggacc ggccgcgacc
     5581 tcgtgagcgg ggagcggcgg cgcgtctcgt cgggcagccc gtacgaggcg cgctacggct
     5641 acagccgggc agtcgcggtg ggcgattcgt gctgggtcgc cgggacgacg gacgcgggcc
     5701 cggacgggat ctcgcgcaac cccggagacg cggcggctca ggcgcgagcc gcctttgcga
     5761 tcgggatcgc cgcgctcgag gaggcgggct tcgccgcggc ggatgtcgtc cgaacccgga
     5821 tgtacatcgt ccggtccggc gacgcggccg cggtcgccgc ggtcaacggc gagctcttct
     5881 cggcgatccg gccggcatcg accctcgtcc gcgtcgccgg cctgatcgag ccgtcgatgc
     5941 tcgtcgaggt cgagctcgaa gctcgccgcg gttgagctga tcgatcgtca gggcagcccg
     6001 gccgcgcggc ccgcgatcgc gatcgcgacg gcggcggcga ggccgacgaa caggctccgg
     6061 cgccaggcga cgatgacgag gcaggcgagg accgacagcc acggcaggcc gacgacgaac
     6121 acccaccgat cgtcgcgcgg cgcgaccatg acctgcgtcg cggcgagggc ggcgaggacg
     6181 gccgggccga cgagctggag atactcgagc gcgcgccgcg gcagccgatc gatcgcggga
     6241 ttgagcaacc cgatcgcccg cgacgggtag gtgaccgcga acatcagcac cgcgagcgga
     6301 acgaggtcgg tgctcatcgc gatcggtcct cgtgcctggc atccgaggtc gccgaccccg
     6361 gcgttcgcgg cacgccatat cgaccggccg attccggcga cccgatcgtc gcaacctcgc
     6421 gcgcctgggc cggcgggatg agcagcccga cgagtggacc gaacagccct ccggcgatga
     6481 tcccgacggt cggactcgag accagcgagg cggccacgcc gatggccgcg ccaccgaacg
     6541 cggcggccag ctcccgccgc ccgctgatca gcccgatcgc caacccgccc atggcggcag
     6601 gggagatcac gtcgatcccg aggcgttggg ggttgacgat cgcatcgccg agcatcaccc
     6661 cggcaaaggt cgccacgttc cacgggatca gcgtcgtgac gattgccccg taccagtagc
     6721 cgatcgcatc gaaccggccg acacgccgga aatgggcgat cgagagggcg aaggcctcgt
     6781 ccgtcaggag atgggcggcg accgcccgct cgacgaagga tcggccgcgg aaccatggcg
     6841 cgagggcggc cgaatagagg atatggcgag cgttcagcag gaacgtcagg aggccgattg
     6901 ccggccagct cagcccggcc gcgacgtagc cgaccgcagc gaactgggcc gcaccggcaa
     6961 acgcgatcac gctcatcgcc atcgcctcga tcggcgagaa gtggccggtc gtgcgggcgg
     7021 tcaggccgaa gacgaagccg aatccggtcg cgctgacgat gatccccagg ccgtcgagcg
     7081 tgagccgccg gcgagcccgt ccgagggcgg cggcgtcgat ctgggccgga acgtcgggtt
     7141 tcagcgcagg aaggtctttc cgcaggtccc gtcgcagacg acgaacagcg cgtgcttctc
     7201 agccgcgtag ccggcgacat ggaggagccc ggggcagccc ggcgtcgggc atttccagac
     7261 ccaccagcgc tcgttgctgt cgagcggcgt ggtctccgcc tcgagctggt cggacaggcc
     7321 gtcccgctcc gcgacgagcg ccccgatccg ggtggctcga tccgcctccg tctcgctggt
     7381 gtacggatag ctctggccga cgttcgaagt cacggacacg gctccaggat cgggaagggg
     7441 gaagaccggt ggcggcgtcg tgtgagcgca gcccgccacc ggtggacgcg aggataactc
     7501 caaagctgtc gcggtggaac gaccagggcg gcgttgcgcc cacggtccgt gctcaggcgc
     7561 gcggtcgcgc gcggtgctgg tctaccagac cgccggcaac ccggcgctcc ggggatcggt
     7621 ggccgcggcc agcgagccgt cggcggtggc cggtccgccg tcgatcagct cgatcgcgtg
     7681 ggcatgtccc agcaggccgt tgaacgggat cgtccgggtg acgggatggc ccatcgcctc
     7741 gagggcgtcg aggaccgccg ccgggtatcg ccgctccgcc cggatgtcga ccggcggcgc
     7801 gaagtgctgg tcgccgtcga cgaaccagcg cggcgcgccg accgtcgtcg cgatgtcgag
     7861 cccgccgtcg accagggcgg aaacgacctg ggcatggatc tgcggctggg cgtcaccgcc
     7921 catcgacccg gtcacgaccc acggccgtcg ctcgccggcc cggaacagca tcccgggcag
     7981 gagtgtgtgg agcgtccgct tgccgggcgc gaggacgttc ggatgcgcgg gatcgaggct
     8041 gaagtacgag ccgcggttct gatagtggat gcccgtgtcg gcatcgacga cgccggaccc
     8101 gaagcccatg tagttcgact ggatcaggct gaccgcgttg ccctccgcgt cgacggacgc
     8161 gagatagatc gtcccgccgc cgcgcgggtt ggtcgatgcc ggcggtcgcg acgcccgggc
     8221 ggggtcgatc cgggcggcaa gctcttcgcc gtacgccttc gaggtgagct ggtcgagcgg
     8281 gatgtcgagg aaggcggggt cggtcagcca gcggtcgcgg tcggccatgg ccagcttggc
     8341 cgcctcgacg ccgagatgga cccagcgggc gtcctcgacg ccatgcgggc cgaacatcga
     8401 cgacgccggt ggttcgaacg tctcgaggat gttcaggagc tcgagcgcga cgatcccgga
     8461 gctgttcggt ggatgggtcg tgacccgcac cccgcggtag tcggtcgcga tcggctcggt
     8521 ccaggtcgag gtgtggctgc gaagatcgtc gacgctgatc gccgaaccga cctcggccag
     8581 gccgcgggcc tggcgctcgc cgatctcgcc gtcgtagaag tcgtcgaagc cgacttcggc
     8641 cagtcgctcg agcgttgccg ccagcgcagg cagcttcacg cgttcgcccg gtcgccaggc
     8701 tcggccgtgc ggtcgataga cccgctcgaa cccggagcgc gggcccagcg ccgcgacgac
     8761 cgccgggagc gtcccctcga ccgccgagat gaagccgtcc caggccggga agccgtccca
     8821 ggccagctcg atcgccggcc cgaggacctc tgcacgcgac agccggccgt gccggcggtg
     8881 cgcgtcgtcc caggagcgga ctgcgcccgg gaccgtgatc gcgagcgggc cgcgccgggg
     8941 gagggtcgcg agaccgcccg ctcggagcgc ggcggcgtca gcacgagcgg gcgcccggcc
     9001 cgagccgttc agggccgtct gccggcctgc ggcctcatcc cagatgagcc agaacgcgtc
     9061 gccaccgagc ccgcaaccgg acggcaggac gacgcccagc accgcgttcg tcgcgatcgc
     9121 cgcgtcgacg gcgtggccgc ctgctcgcag gatcgcgaga cccgccgcgg tcgcgaggtg
     9181 gtggggcgcg acgaccgcgc cgtgggcgcc gcggacgagc ggcatcggca caagcggacg
     9241 gtcgggcatg gccggattgt agggggcggc tcccgttacg cttgcgccgt gcgatcgctc
     9301 tacgctgtcc tcctcgggac gttcacgctc cggttcagca ccggactgac cggcggcctg
     9361 ttcctgttct acctctccca cctcgagaag gattacggcg gcccgcggac cgacgagacg
     9421 accctcgccg tgatggtcgc cctgttctac gcgtccgagc tcgtcctctc aacgccgttc
     9481 gggctcctgt cggaccggct cggccaccac cgcgtgatgc agatcgggcc gttgttcggg
     9541 atcatcgcct cgctcgtcac atggctgtcg gtcaacctgg tcgtgatcgg tgggactcgg
     9601 ctcctcgagg gcgcctcgac cgcggcctcg gtcccgtcga tcctcggctt catcgcgatg
     9661 ttcaccgcgg gcaacgagct cttgcgcggg cgcgcctcgg cccggttcga gggcgcgacc
     9721 ctggccggca tcggcgtggg agccgtcgcc gccggtgtcc tctacgacgg gatccccggt
     9781 gtctggggcg gcctccaccg cgacgccttc ctgctcaacg ccgggttcta cgtcatctcg
     9841 ctgctgatct accgctacgg cgtcggcgag ccacccggcg aggccgaggc cgtcggcggc
     9901 gaacacaccg gctggcggcg ctactcggcg gtcctgctga gctcccacgt ctggctgctt
     9961 gccccgacgt ggatcgcgat caacgcggcg ctcggcctgt gggccagcca gtcgatcttc
    10021 cagctgatca aggtgcccga tccacggttc gccgaccagg ccctgatggg cgggttcgtc
    10081 ggcgcccaga tcgcgctcgg gcttggcgtc gccctgatcg tgttcttcgc cgggctcgtc
    10141 ttctggggcg atcgattcaa gcgcttccgg cggacgacga tcatcgtcta cggcatcggt
    10201 ggtggggccc tgagcgtcat cggcgcggcg atcgtcaacc acggtgcgga cctggtgccc
    10261 gtggccctcg ctgcggccgt gctggccggg gtcggcctgt tcgtcctggc cggggcgaca
    10321 ccggccgcgc tcgggctgct cgccgacatg agcgaggcct atccctcgga tcgcggcgcg
    10381 atcatgggcc tctactcggt gttcctcgcg gtcggccaga tcatcggcag cttcgtcggc
    10441 gccgaggccg ccaagcacta cggcatcgac ggcatcctcg tcgcgacgct cgggttgctc
    10501 gcgatcgcgc tcgtcccgat ccgtcgactg cggccattcg agcaccgggt cgggagtgcg
    10561 gcgatggccg gcagccgggc ggcctgaggc gcgacgcgcc cgcccgacgt gttcgggcga
    10621 atgcggtccc gtatcatcgc gacggggatc gccactctgg agggagccgt ggcccgcatc
    10681 gtcttcgtca ccgactcggc gtcggacctc gacccggcgg tcgccaccgc ccttggcatc
    10741 cgggtcgtgc cgctcgtggt gacgttcggc tccgacacgt accgggccgg cgtcgacctc
    10801 tcgacggccg cgttctggga gcggatgacc gcgccggacg caccgttccc gaagaccgcc
    10861 gcggcgagcc ccggcgacgt gaagtcgatc tacgacggcg cgttcgccga gggcgccgac
    10921 gcgatcatct cggtccacgt cgccggcacg ctgtcgggcg cgatcaagag cgccgagatc
    10981 gcgaagtcga tgctgcccga ccgggagatc cacatcgtcg attcgaccgg cgcctcgatg
    11041 gccgagtcga ttctctgctt catgggccag gagctcgcgg ccggtggcat ggccgccgcc
    11101 gagatcgccg agatcctgtc cgatcgagcg tccgatctcc agatatacgt ctcgctcgag
    11161 accctcgcgt acctcaagaa gggcggccgg atcagcggcg cccaggcggc gatcgggacc
    11221 ctgctctcgg tcaagccgat catcggggtc gaggccggca aggtcgagat cgccgacaag
    11281 ccgcggaccc gctcgaagtc gcgccagcgc tgcatcgaga tgatcaccgc ccgcccgatc
    11341 gagcgggtcg cgatcctcca cacgatggcc ccggacgtcg aggcgttccg cgacgaggtc
    11401 attcgccggg ccgggctcga cgcatcggac gtgacggtct cgatcgttgg accgtcggtc
    11461 gggccgcatc tcggcccggg gtgcgtcggt gcggcggtgc tctaccagcg ctagcgttcg
    11521 gccaggcgcg cggctcgaac ctctggagtg aggtgcgccc gctccgggtg cgagcggccc
    11581 agaggaatct cctgcattca ggcgagcgtc gcctgcggaa ggcaaccacg tgcggcaccc
    11641 aactgctcgt cacgctcgga tcggtgacgg tcgagctgtc actcaggcga gcgtcgccta
    11701 cgcgcaagag atccgcccgc gggcgtccgc aacaggcgag cgtcgcctac gcgcaggaga
    11761 tccggcccgc gggcgtccgc atgggctggg cgcagggctg aatggcccgg gttgcggtgc
    11821 ggttgcgccc gggttgcgac atggagaatg gcacgcggcc agatccggtc gtcgtggcta
    11881 tactcggggt ggctcttgac gcgccgtccg ccactttgac gctggcggcg ccccgtgggg
    11941 tcgtccggga gacgacccac atctcgggcc ggtccagggg accggtctcc acggaggtcc
    12001 gcctcacacc atgacgatcg agcaagtcgc cgcgtcgatc aacccgtggc tggtcgccca
    12061 acagcagttc gacctcgccg ccgagcgcct gaacctcgac cccggcctcc gtcgcgtgct
    12121 ccgcgagccg cgccgcgagc tgacggtcca cttcccggtg aagatggatg acgggtcggt
    12181 ccaggtctat accggctatc ggatccagca caacctcagc cgcggccccg caaagggcgg
    12241 catccgctac caccagaacg tcagcctcga cgaggtcaag gccctcgcga tgtggatgac
    12301 ctggaagtgc gcggtcgtcg gaatcccgta cggcggcggc aagggcggcg tgatcgtcga
    12361 ccccaagaag ctcagccaga aggagctcga ggcgctgacc cgacgctact tcaccgagat
    12421 cgaggtcctg atcggaccgg agcgcgacat tccggcgccc gacgtcaaca cgaacggcca
    12481 gatcatggcc tggatgatgg acacctattc gatgcacgcg ggccacacgg tcccgggcgt
    12541 cgtgaccggc aagccgatca gcatcggcgg gtccgagggt cgcaacgagg cgaccgcgcg
    12601 cggcaccgtg ttctgcatcg tcgaggcggc ccgccacctc gggatcgagc tgaacaaggc
    12661 caaggtctcc gtccagggct tcggcaacgc cggctcgatc gccgcgacgt tgatcgtcga
    12721 ggagggcgcg accgtcgtcg cggtctccga ctcgaccggc gggatccaca acccgaacgg
    12781 gctcgacatc aagcgcgtga tcaactggaa gaaggagcac gggaccgtcc agggcttccc
    12841 gggcgcgacc gacatcagca atgaggccgt cctcgaagtc gagtgcgaca tcctgatccc
    12901 ggccgccctc gagaaccaga tcaccaacca caacgtcgcc aacatcaagg cccgcctgat
    12961 cgccgaggcc gccaacggcc cgacgacccc ggacgccgac caggcgatgt tcaaggccgg
    13021 gaagttcatg atcccggaca tcctgtgcaa cgccggcggc gtgaccgtca gctacttcga
    13081 atgggtccag gacctcaacc gcgatcactg gagcgagagc gtcgtcaacg ccaagctcaa
    13141 ggagatcatg gtcaaggcgt tcagcgagac tctcgcgatc gcccgtcggg agcagtgcga
    13201 catgcggacc gccgcctatc tgctggccgt ccagcgagtc gccgacgcca ccgcgctgcg
    13261 cggcctctac ccatagatcg actcaccgcc cacctcgaag ccccggtcat cgaccggggc
    13321 ttcgtgattc ggcgtgtcat gatcgacaac ggtgggcaac ccggctccac cgccggctgc
    13381 gccccgggtg agcgttgcga cgaaggaccg atggaaatgg gccgcgccgc gcgccggaat
    13441 cggcattccg gtgccgtctt gcgcgccgag tgcgcagcgc agcgcccggc agcacggccg
    13501 ttcgccgcag tgcgcgttcc gcgcgcatcg ggcccggcct cgggcccgag aacaggcggg
    13561 actcgattct ccccggatcg ggcggctgat accatcccga ccatgctcga ccacgaagag
    13621 aagttcctgt tcaaggagga gatcctccac ctcgcgaacg cggtggcgga gcccggcacc
    13681 ctcgacaacg tcgaggacct cctgaccgac gtcatcacca gtccgcggtt cgactccgag
    13741 gtcttcaccc tcgagcggag cctccaggtg atgctcgggc cgcgggcggg gacgaccctc
    13801 gtggggctgc tgaccgtcgc gcccgaggtc ttcgaggagg tcgccgacgt caacatcccg
    13861 tcggaggtcc acgagcggct tgtcgcctgg cgggcgcggt tcgggctggc gatcgacaac
    13921 gccctgaact tcctcaacaa ccccgacggc atccagggcc acaacttcgc cacccaggtc
    13981 gcccacgtcg aggagcggcg cgtcctccag ggccgcctga cgctcgtccg ctacaacaac
    14041 gagccgcagt tcttccag
//
LOCUS       DATAIW010000264        12944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_45626, whole genome shotgun sequence.
ACCESSION   DATAIW010000264 DATAIW010000000
VERSION     DATAIW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45626"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /gene="nusA"
                     /locus_tag="VHM48_08365"
     CDS             complement(<1..1164)
                     /gene="nusA"
                     /locus_tag="VHM48_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX2755462.1"
                     /translation="MSRDFVGALLQLNAEKQVSREQLIHAVEDGIQSAYRRVAGDEDI
                     HVRIDAETGKIRVFRARLAVDEVEDPLIEFTLEEARALKADAQPGELVETEELPDDVF
                     GRIGAQTAKQIVLQRIREVERDQVFDKFANREGEMITGAVNRVEPRAIILDVGNNVEA
                     VLSTSEQSTVEHYRIGQNVKAFVLEVRRSSRGPQILVSRTHKGFLKRLFELEVPEIHS
                     GTVEIKAIAREPGSRSKVAVSSRQEGLDPVGATVGQRGARVQAVVAELGGEKIDIIPW
                     NDDASVFVANALSPAQVISVDIDEEKRIANVTVPERMLSLAIGREGQNARLAARLTGW
                     RIDIRSDVSVAAKAAEDEAAAAPAAPVAKAVVVETATTTAAAAIEKAAPKPRAK"
     gene            2018..3082
                     /locus_tag="VHM48_08370"
     CDS             2018..3082
                     /locus_tag="VHM48_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755463.1"
                     /translation="MIGWGRVSQPGRNQFNDMGAMYLSSDGINWRVTPIDAGVRRQDA
                     SEFSLVADGPAGILILGNVCCTEEERPALWLAADGGTLDRLPWPDTFGVRSEISTLAA
                     TESLYLAGGTAAGSAAVWSSPDGAAWTRIEDPAAGLGAGGLFDIEPAADGLVAVGWQD
                     VDGTSDGAVWTSPTGAEWRRLPVALLEGPLETTIYRVVPWAGGWFLIGQEGTHADRIA
                     CEGIARVASVSDPEGGNAPPPDFSCGWGVETHWLTADGRDWQRDIPIGAQPGAVIPDG
                     ALIELRLISAGGPGLVVLGEGSRVGSAGIFVSADGIRWQVTAPGGVFPGGSIPNGLAV
                     GGRTIVAVVEGPSAWIGLAR"
     gene            complement(3111..4367)
                     /gene="ispG"
                     /locus_tag="VHM48_08375"
                     /gene_synonym="gcpE"
     CDS             complement(3111..4367)
                     /gene="ispG"
                     /locus_tag="VHM48_08375"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2755464.1"
                     /translation="MTTATDALRPRRRSTVSVDVGGVLVGSAHPIVVQSMTNTDTADP
                     DATAIQVARLAHAGSQLVRITVNTEQAAAAVPEMMRKLRDNLGVEVPVIGDFHYNGHK
                     LLVEYPDMARALAKYRINPGNVGAKRHDEHFATIVQVAIDNHKPVRIGVNWGSLDPQL
                     LTDLMNANAVAAEPRDARDVMIEAMIESAMRSAELAETTGLRHDQIIISAKVSQVRDL
                     VDVYRLLAARSDYPLHLGLTEAGMGSKGIIASTAGLAILLNEGIGDTIRVSLTPAPGG
                     DRAEEVEIAQQVLQSMGIRSFLPQVSACPGCGRTTSTFFQEMAQQIQQHLKDRMPGWQ
                     ETHPGVEEMRVAVMGCVVNGPGESKHADIGISLPGTFEDPVAPVFIDGKLDRTLRGES
                     LVKEFIDILEDYVDSHYPASGVPAGV"
     gene            complement(4371..5516)
                     /locus_tag="VHM48_08380"
     CDS             complement(4371..5516)
                     /locus_tag="VHM48_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX2755465.1"
                     /translation="MTGPLGVLVTIVLFIVVLGSLVLIHEIGHFVMARLAGIRVLEFG
                     VGFPPRAKVLRSRGETLYTLNWLPIGGFVKLEGEDGDENEDPRSFARAKLPVKMIILV
                     AGVAMNLLLSLVIFTSIAWLASPVVGLTIPVVEPGSPAERAGLKANDAIVSVDGIRHD
                     IFSLASGDVIEDLRDRVGQTVTIQVVHPGGVRVDVPVTLRTAAEMKDPDGDGPLKAKG
                     ALGISVADPKAGWPTFFSGDYLQRDLPSAISVGSHETVRWFGVILGGLGDLVRQVAAN
                     PTAAPPVSGPIGIATTISDIFFGAGIVMTLYVAGILSANLALVNILPFPPLDGGRMLM
                     IVLKRIFGARISLRAEQMTYMVGFVFLFAFLIWVSGFDIVRILSGGS"
     gene            complement(5513..6733)
                     /gene="dxr"
                     /locus_tag="VHM48_08385"
     CDS             complement(5513..6733)
                     /gene="dxr"
                     /locus_tag="VHM48_08385"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2755466.1"
                     /translation="MSSPRSAEPGGRLRVAVLGSTGSIGTQTLDVLAAHPDAFEVVAL
                     AAGANAALLAEQAARFRPAAVAVSGDAPLGLPGGTRRESGSGPEALTNLATRDDVDLV
                     VVATGGIVSLRPVLAALGAGKVVATANKETLVAGGHLVMPLARALAAARDAADPFASP
                     LAWLRPIDSEHSAIWQCLVGESLGRIASLVLTASGGPFLDGPADLSAITPRQALRHPT
                     WSMGAKITIDSATLANKGLEVVEARWLYDVGDDEIEVVIHPQSVVHSAVRFVDGSLKA
                     QLGTPDMRTPIQYALTYPDRRPSPSAAVDLVAAGRLDFRAPDETRFPALRIARDAGRI
                     GQRATTALIAADDIAVARFLGGTLGFNGIPALLAAAVDRFGQGPDQAPAVDALVALDT
                     EVRAFAAGWHGAGS"
     gene            complement(6711..7523)
                     /locus_tag="VHM48_08390"
     CDS             complement(6711..7523)
                     /locus_tag="VHM48_08390"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2755467.1"
                     /translation="MSAAVLIPPLVVALLLGGPWIVAVVVAATALAAREVFQLLTGAG
                     YPSLAWLGIVFAVALVLDAAIMPAIDASGVLLVATGTILAAVGAFTRTDPRDGLATWF
                     GTVFGAFYASLLGFVLRLGHEAPALPDSAPLAGLGADRGWIVLLVLAVWSYDTGAYFI
                     GKRFGRRRFLTHISPSKTYAGLIGGVVAATIVSGLILVGLGQQAVTALIVGPLVSLAA
                     QAGDLAESMLKRAAGAKDSGQLIPGHGGVLDRVDSFLFAAPVLALYVLAAVR"
     gene            complement(7568..8374)
                     /gene="uppS"
                     /locus_tag="VHM48_08395"
     CDS             complement(7568..8374)
                     /gene="uppS"
                     /locus_tag="VHM48_08395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2755468.1"
                     /translation="MARTPLARPTDAPPTAHRPDAEVAAQPASPAAPDAERLLPDSDL
                     PRHVAIIMDGNRRWARQRGLAELDGHAAGVEAIRTLLRHAVRRGVPVVTVYAFSRENW
                     ARSDEEVRGLFALLEQAIRSETAELKTQGVRIRLLGRLDELPDDTRRSIGEALAETAG
                     GDRLLLNVAFNYAGRTELVDAVRRIVASGRAAESIDENAIADALYTAGLPDPDLVIRT
                     GGEQRLSNFLIWQAAYAELYTTEVLWPDFGADAFDAALLEFASRTRRFGR"
     gene            complement(8374..8931)
                     /gene="frr"
                     /locus_tag="VHM48_08400"
     CDS             complement(8374..8931)
                     /gene="frr"
                     /locus_tag="VHM48_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2755469.1"
                     /translation="MSSETISSADHKMNRAVDAMERDFQAFRTGRASTALVERLTVDY
                     YGTQTPLNQLAGISVPEAHQIVIQPWDRGVLGAIEKAIQKSDIGLMPNVDGTVVRLNI
                     PPLTEDRRKELVKSVHRRMEEAKVEIRNLRREAADDLKKEERDGTVGTDESHRQLEQL
                     QKTTDRWIGEVDRVGASKEQEVMEV"
     gene            complement(8928..9716)
                     /gene="pyrH"
                     /locus_tag="VHM48_08405"
     CDS             complement(8928..9716)
                     /gene="pyrH"
                     /locus_tag="VHM48_08405"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2755470.1"
                     /translation="MSDAPGATTATSPAAGEKVRYSRILLKLSGEALLGNRSYGVDPA
                     FCAFIARQVAEVHRLGVQVGIVVGGGNIFRGLAAAASGMDRATGDYIGMLATVMNGLA
                     LQDALERAGVPTRVMSAIAMNEVAEPYIRRRAVRHLEKGRVALFVAGTGNPYFTTDTA
                     AALRAVEIGAEVLLKATKVDGVYDADPQTHPNARRYAELEYADLLRDQLKVLDAAAVS
                     LCMENDLPIVVFDLNQPDNITRVATGEPVGTRISGAAGARGGTR"
     gene            complement(9713..10312)
                     /gene="tsf"
                     /locus_tag="VHM48_08410"
     CDS             complement(9713..10312)
                     /gene="tsf"
                     /locus_tag="VHM48_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2755471.1"
                     /translation="MADISAAAVKDLRERTGAGFMDCKNALVASDGDVEKAVALLREK
                     GLAAATKKAGRDAREGLVSSYIHTGGRVGVLIEVNCETDFVARTDEFQKLVRDLAIQV
                     AGMQPLYVDADRIPADVLEARKSELLSDEATQKKPEAIRPQIVEGQLKKWFSQVCLYD
                     QPFRDEERLVRDLVTEKISTIGENIRVRRFVRYALGEEL"
     gene            complement(10455..11411)
                     /gene="rpsB"
                     /locus_tag="VHM48_08415"
     CDS             complement(10455..11411)
                     /gene="rpsB"
                     /locus_tag="VHM48_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2755472.1"
                     /translation="MPNVSMRQLLEAGVHFGHQTRRWNPKMKPFIFAERNGIHIIDLA
                     QTVKHLESSLAFVRETVAHGEYVLFVGTKKQAQEPIAQEATRAGQPFVNKRWLGGMLT
                     NFSTIKKRISLLEQLEARQQAGDFERMTKKEAAKLTDEMNKLTLTVGGMRKMKRVPGA
                     IFIIDPHRERIAVTEANKLELPVVATGDTNVDPDEIDHIIPANDDAIRAIRLLCTLIA
                     DACIEGQQERAARASEPEPEVAMATEPEYDESASNELVAALAGGAALSFEPDADEDEL
                     LPGARPVAPVAETAPAAEASPEEIAAELAREAAEKEEPASAG"
     gene            complement(11805..12197)
                     /locus_tag="VHM48_08420"
     CDS             complement(11805..12197)
                     /locus_tag="VHM48_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX2755473.1"
                     /translation="MRTDAQASGDDAESEVAAGLARQGWTIMARNLRVGRGELDIVAV
                     DPGPPAELVVVEVRWRRSRAFGLAEESIDHRKRAHLRAAIGRLLEDGLPNVGRLPPLP
                     IRVDLVVVEPPIAPGESARIRHHRAIPL"
     gene            complement(12233..12820)
                     /locus_tag="VHM48_08425"
     CDS             complement(12233..12820)
                     /locus_tag="VHM48_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2755474.1"
                     /translation="MARAVAVGAGAASVREIDQLNIYHATHLAMRRAIARLGGHEHVM
                     VDGNRIAGFEALVGPYTNIVDGDAKVYSIACASVVAKVVRDRMMSLLAARYPGYGWEH
                     NQGYATLDHRLAIRAMGLTPFHRRSFLALQRTLAGDQLDFDLLGDPGASPDTHELLDR
                     ESVSVMADDAMLDAADLAALDMVAFDDAPLEPVAG"
BASE COUNT         1890 a         4399 c         4669 g         1986 t
ORIGIN      
        1 cttcgcacgt ggcttcggag cggccttctc gatggcggca gcagcggttg tcgtggccgt
       61 ctcgacgacc acggccttgg cgaccggggc tgccggagcc gcagccgcct cgtcctcggc
      121 cgccttggcc gcgaccgaga cgtcgctccg gatgtcgatc cgccagccgg tgaggcgggc
      181 ggcgagtcgg gcgttctggc cttcgcggcc gatcgccagc gacagcatcc gttcgggaac
      241 ggtcacgttg gcgatccgct tctcctcgtc gatgtcgacg ctgatcacct gggccggcga
      301 cagcgcgttg gcgacaaaga cgctggcgtc gtcgttccac gggatgatgt cgatcttctc
      361 gccgccgagc tcggcaacga cggcctggac ccgggcaccg cgctggccga cggtcgcgcc
      421 gaccggatcg agcccttcct gacgcgacga gaccgcgacc ttcgagcgcg agccgggctc
      481 gcgggcgatc gccttgatct cgacggtgcc gctgtggatc tcgggcacct cgagctcgaa
      541 caggcgcttg aggaagccct tgtgggtccg gctgacgagg atctggggac cgcggctgga
      601 gcgccggacc tcgagcacga acgccttgac gttctggccg atccggtagt gctcgacggt
      661 cgactgctcg ctggtcgaga ggacggcctc gacgttgttg ccgacgtcga ggatgatcgc
      721 ccgcggctcg acccggttga cggcgccggt gatcatctcg ccctcgcgat tggcgaactt
      781 gtcgaagacc tggtcgcgct cgacctcgcg gatccgctgg agcacgatct gcttggcggt
      841 ctgggcgccg atccggccga acacgtcgtc gggcagctcc tcggtctcga ccagttcgcc
      901 gggctgtgcg tcggccttga gggcgcgcgc ctcctcgagg gtgaactcga tgagcggatc
      961 ctcgacctcg tcgaccgcga ggcgggcccg gaagacccgg atcttgccgg tctcggcgtc
     1021 gatccggacg tggatgtcct cgtcaccggc gacccgccgg taggcggact ggatgccgtc
     1081 ctcgacggcg tggatcagct gctcccggga gacctgcttc tcggcgttga gctggaggag
     1141 ggcaccgacg aaatcgcgac tcacgatgcc tccttgctgc tgcacggttt ccggtgggcc
     1201 gacataaaga aacgtggggg tcacccacgc ctggagcggc cgttaagttg catggaacta
     1261 tacacccgga tgggtggacg gctcaaacga gaggcacgcg tgggaccggc gcacgccagt
     1321 tcgttcagtc gcgcgtccgt ctcttccttt tgacagccag gacggccata tccacgttgg
     1381 acaagcgccg ccgaccctca tccgccccaa actccgatgt ggcggtcaaa tgagagccat
     1441 tcgcgccgat cagccgttgt cgcctgcgcg gcgccgccca aggcggtcgt ggctgtcgtt
     1501 tgatcgaacg cgtcagctcg ccaggatcgc cgatcgcgcc ggataccggc gcagggcccg
     1561 gttcttccct gcgctggtga agctgccgat cgcctcatcc caaggggccg gcgggccgcc
     1621 gtcgccaccg acacggtcca tcatgcgtcc aaccggcgaa cgccactccc aaccggaggc
     1681 cctcggaccg atgacgcgaa ccgggcaacg gcacccgcgc ggagtctggc tcctcgtggt
     1741 tggggccggt ctcgtgaccg tgctcgcggc cggcttcgcg gtcagccgcc cagtggccac
     1801 accgatggat ggtccgagtt cgtcgccggg cgtcgcctcg gcaccgggct cgtcatcgga
     1861 ccttcccgtc gtcggacctc tcggcggcgt caggatcccc ccgcgcgacc cggaaccctg
     1921 gaccgcggtc gactggcgcg aggtcccgcg ggcgttcggg gccaaacccg accgcgccct
     1981 ggaccgcatc gacggcacga tcgtcggtcg acatcgcatg atcggctggg ggcgcgtctc
     2041 ccagccgggt cgcaaccagt tcaatgacat gggcgcgatg tacctgtcct ccgacggaat
     2101 caactggagg gtcacgccga tcgacgccgg cgtgcgccgt caggatgcgt cggagttctc
     2161 tctggtggcc gacggaccgg ccggcatcct gatcctcggc aacgtctgct gcaccgagga
     2221 ggaacggccg gcgctctggc tggcagccga cggcgggacg ctggatcgct tgccgtggcc
     2281 cgacacgttc ggggtccggt cggagatcag cacgctcgcg gcgacggaat ccttgtacct
     2341 cgcgggcggt acggcggcag ggtcggcggc cgtgtggtcg tcacccgacg gcgcggcgtg
     2401 gacccgcatc gaggatccgg ccgccggact tggcgccggc gggctcttcg acatcgagcc
     2461 ggccgcggac ggcctcgttg ccgtcggctg gcaggacgtc gacgggacgt ccgacggcgc
     2521 cgtctggacc tcgccgaccg gcgcggagtg gcgacgcctg ccggttgccc tgctcgaggg
     2581 tccgctcgag accacgatct accgcgtcgt cccgtgggcc ggcggatggt ttctcatcgg
     2641 ccaggaggga acccacgcgg accgcatcgc ctgtgagggg atcgcccggg tcgcctcggt
     2701 gtccgatccg gagggaggca atgcgccgcc cccggacttc agctgtggtt ggggtgtcga
     2761 gacccattgg ctcacggccg atggtcgcga ctggcaacga gacatcccga tcggcgccca
     2821 gccgggcgcg gtcatccccg atggcgcgct gatcgagtta cggttgatca gcgccggcgg
     2881 gccggggctc gtcgtgctcg gcgagggctc gagggtcggg tcggcgggga tcttcgtgtc
     2941 cgccgacggg atccgctggc aggtgacggc tccaggcggc gtgttcccag gcggcagcat
     3001 tccgaacggg ctcgcagttg gaggacggac gatcgttgcc gtcgtggagg gcccgagcgc
     3061 ctggatcggc ctggcgcgct gagccccgat acgccccggg tgacgagccg tcagacccct
     3121 gctgggacgc ccgacgccgg gtagtggctg tcgacgtagt cctcgaggat gtcgatgaac
     3181 tccttgacga ggctctctcc gcggagcgtt cggtcgagct tgccgtcgat gaagaccggc
     3241 gcgaccgggt cctcgaacgt gcccggcagc gagatcccga tgtcggcgtg ctttgattcc
     3301 cccggcccgt tcacgacgca gcccatcacc gcgacccgca tctcctcgac gccgggatgt
     3361 gtctcctgcc agccgggcat ccggtccttg agatgctgct ggatctgctg ggccatctcc
     3421 tggaaaaagg tcgacgtcgt ccggccgcag ccgggacagg cgctgacctg gggcaggaac
     3481 gagcggatgc ccatcgactg gaggacctgc tgggcgatct cgacctcttc cgcacggtcg
     3541 ccgcccggcg ccggcgtcag ggagacccgg atcgtgtcgc cgatcccctc gttgagcagg
     3601 atcgccaggc cggccgtcga ggcgatgatc cccttcgagc ccatccccgc ctcggtgagc
     3661 ccgagatgga gcggatagtc ggagcgggcc gccagcaacc ggtagacgtc gaccaggtcg
     3721 cgaacctgcg acaccttggc gctgatgatg atctggtcgt ggcggagccc cgtcgtctcg
     3781 gccagctcgg ccgaacgcat cgcggactcg atcatcgcct cgatcatcac gtcgcgggca
     3841 tcgcgcggct cggcggccac cgcgttggcg ttcatcaggt cggtcaggag ctgcgggtcc
     3901 agcgagcccc agttgacccc gatccggacc ggcttgtggt tgtcgatcgc gacctggacg
     3961 atcgttgcga agtgctcgtc gtggcgcttc gctccgacgt tgcccgggtt gatccggtac
     4021 ttcgcgaggg ccctcgccat gtccgggtac tcgaccagga gcttgtggcc gttgtagtgg
     4081 aagtcgccga tcaccgggac ttcgacaccg aggttgtcgc ggagcttgcg catcatctcg
     4141 gggaccgcgg cggccgcctg ctcggtgttg acggtgatcc ggaccagctg cgacccggca
     4201 tgggcgaggc gggcgacctg gatcgcggtc gcgtcgggat cggccgtatc ggtgttcgtc
     4261 atcgactgga cgacgatcgg atgggcgctc ccgacgagaa ccccgccgac gtcgacgctg
     4321 acggtcgaac gccgccgtgg ccggagggcg tccgtggcgg tcgtcatcgc ctacgacccg
     4381 ccggacagga tccggacgat gtcgaagccg ctcacccaga tcaggaacgc gaacaggaag
     4441 acgaacccga ccatgtacgt catctgctcg gcccgcaggc tgatcctcgc gccgaagatc
     4501 cgcttgagca cgatcatcag catccggcca ccatcgagcg gcgggaaggg caggatgttg
     4561 acgagggcaa ggttcgccga gaggatgccc gcgacgtaga gggtcatcac gatcccggcg
     4621 ccgaagaaga tgtcggaaat cgtcgtcgcg atgccgatcg ggcccgagac cggcggggcc
     4681 gcggtcgggt tggccgcgac ctgccggacg aggtcgccga ggcccccgag gatcaccccg
     4741 aaccagcgaa ccgtctcgtg gctcccgacc gagatcgcgc tgggcaggtc acgctggaga
     4801 tagtcccccg agaagaaggt cggccagccg gctttcgggt cggcgacgga gatcccgagc
     4861 gcccccttcg ccttcagcgg tccgtcgcca tcggggtcct tcatctcagc cgcggtccgg
     4921 agggtgaccg ggacgtcgac ccggacaccg cctgggtgga cgacctggat cgtgacggtc
     4981 tggccgaccc ggtcgcggag gtcctcgatc acgtcgccgc tcgccagcga gaagatgtcg
     5041 tggcggatgc cgtcgaccga gacgatcgca tcgttcgcct tcagcccggc ccgctccgcc
     5101 ggcgaacccg gctcgacgac cgggatcgtg agcccgacga ccggtgacgc cagccacgcg
     5161 atcgacgtga agatgacgag cgacaggagc aggttcatcg cgacgcccgc gacgaggatg
     5221 atcatcttga cgggcagctt cgcgcgggcg aacgagcgcg gatcctcgtt ctcgtcgccg
     5281 tcctcgccct cgagcttgac gaagccgccg atcggcagcc agttcagcgt gtagagggtc
     5341 tcgccgcgcg accgcaggac cttggcccgc ggcgggaagc cgaccccgaa ctccagaacc
     5401 cggattccgg ccaggcgggc catcacgaaa tggccgatct cgtggatcag gacgaggctg
     5461 ccgaggacga cgatgaacag gacgatcgtg acgaggacgc cgaggggacc cgtcacgaac
     5521 ccgctccatg ccagccggcg gcgaacgcgc ggacctcggt gtcgagcgcg accagcgcgt
     5581 cgacagcggg tgcctggtcg gggccctggc cgaaccgatc gaccgcggcc gcgaggagcg
     5641 ccgggatccc gttgaagccg agcgttccgc cgaggaagcg ggcgacggcg atatcgtcgg
     5701 cggcgatcag cgcggtcgtc gcccgctgtc cgatccgacc ggcatcgcgg gcgatccgca
     5761 gggcggggaa ccgcgtctcg tcgggggcgc ggaagtcgag ccggccggcc gcgacgagat
     5821 cgacagcggc tgacggcgac gggcggcggt cgggataggt cagggcgtac tggatcgggg
     5881 tgcgcatgtc cggggtgcca agctgggcct tcagggagcc atccacgaag cggaccgccg
     5941 aatggacgac gctctgggga tggatgacga cctcgatctc gtcgtcgccg acatcgtaca
     6001 gccagcgtgc ttcgacgacc tccaggccct tgttggcgag ggtcgccgaa tcgatcgtga
     6061 tcttcgctcc catcgaccag gtcgggtggc ggagggcctg ccgcggggtg atcgcggaca
     6121 agtcggcggg gccgtcgagg aatggaccgc ccgacgccgt caggacgagc gacgcgatcc
     6181 gtccgagcga ttcgccgacg aggcactgcc agatcgccga atgctccgag tcgatcggcc
     6241 tgagccaggc cagcgggctc gcgaacggat cggccgcatc gcgcgcggcc gcaagggccc
     6301 gggcaagggg catgacgagg tggccgcccg cgacaagggt ctccttgttg gccgtcgcga
     6361 cgaccttgcc ggctccgagg gcggccagca cgggccggag gctgacgatg ccgccggtcg
     6421 cgacgacgac gagatcgacg tcgtcacggg tggcaaggtt cgtcagcgct tccgggccgc
     6481 tcccggactc gcgccgggtc ccgcccggga gaccgagcgg cgcatcgccg gagaccgcga
     6541 ccgccgccgg acggaatcgg gctgcctgct cggcgaggag ggcggcgttg gcgccggccg
     6601 cgagcgccac gacctcgaat gcgtcgggat gcgccgccag gacgtcgagc gtctgcgtcc
     6661 cgatcgagcc tgtcgagccg aggacggcga cccgcagtcg cccgcccggc tcagcggacc
     6721 gcggcgagga catacagcgc caggaccggg gccgcgaaca ggaacgagtc gacgcgatcg
     6781 agaacaccgc cgtggcccgg gatcagttga ccggaatcct tggcgccggc ggctcgcttc
     6841 agcatcgact cggcgaggtc gccggcctgc gcggcgagcg acaccagcgg cccgacgatc
     6901 aatgccgtca ccgcctgctg gccgagcccg acgaggatca ggccgctgac gatcgtcgcg
     6961 gccacgacgc cgccaatgag cccggcatag gtcttcgagg gagagatgtg ggtcaggaac
     7021 cggcgccgtc cgaagcgctt gccgatgaag tacgccccgg tgtcgtacga ccagacggcg
     7081 aggacgagga gcacgatcca gccgcgatcc gccccgagcc cggcgagtgg tgcactgtcg
     7141 ggcagggccg gcgcctcgtg gccgagccgc aggacgaacc cgaggagcga tgcatagaac
     7201 gccccgaaga ccgtgccgaa ccaggtggcg agcccgtcgc ggggatcggt cctcgtgaac
     7261 gccccgacgg cggcgaggat cgttcccgtc gcgacgagca ggacgccgct cgcgtcgatc
     7321 gccggcatga tcgcggcatc gagcaccagc gcgacggcaa acacgatccc gagccaggcg
     7381 agcgacggat agcccgcgcc ggtcaggagc tggaacacct cgcgggcggc gagcgcggtc
     7441 gcggcgacga cgacggccac gatccacggc ccgccgagga gcaacgccac gacgagcgga
     7501 gggatcagga cggccgcgct cacggcgcgc tgcctgagca agccttccgg gggcggcgag
     7561 atgccgttca gcgtccgaat cggcgggtcc gactggcgaa ctccaggagc gcggcgtcga
     7621 acgcgtccgc cccgaagtcg ggccacagga cctcggttgt gtaaagctcg gcgtaggcgg
     7681 cctgccagat caggaagttc gacaggcgct gctccccgcc ggtccggatc accaggtccg
     7741 ggtccggcag gccggcggtg tacagggcgt cggcgatcgc gttctcgtcg atcgactcgg
     7801 ccgctcgacc gctggcgacg atccgccgga cggcgtcgac gagctcggtc cggccggcat
     7861 agttgaaggc gacgttgagc agcaaccggt caccgccggc ggtctcggcg agggcctcgc
     7921 cgatcgagcg ccgggtgtcg tccggcagct cgtcgaggcg gccgaggagg cggatccgga
     7981 caccctgggt cttgagctcg gcggtctcgc tccggatcgc ctgctcgagc agggcgaaca
     8041 ggccgcggac ctcttcgtcc gagcgggccc agttctcgcg gctgaaggcg tacacggtga
     8101 cgacgggaac cccgcgccgg acggcgtgcc gaaggagcgt ccggatggcc tcgacgcccg
     8161 ccgcatgacc atcgagctcg gcgagtccgc gctggcgcgc ccagcgccgg ttgccgtcca
     8221 tgatgatcgc gacatggcgc ggcaggtcgc tgtcgggaag gagccgttct gcgtcgggag
     8281 cggccggcga ggcgggctgg gcagcaacct cggcgtcagg ccgatgcgcc gtcggtggcg
     8341 cgtcggtcgg gcgggcgagg ggcgtgcgag ccactagacc tccatgacct cctgctcctt
     8401 gctggctccg acgcggtcga cctcgccgat ccagcggtcc gtcgtcttct ggagctgctc
     8461 gagctggcgg tgggactcgt cggtgccgac cgtcccgtcg cgctcttcct tcttgagatc
     8521 gtccgcggcc tcgcgacgga ggttgcggat ctcgaccttc gcttcttcca tccgcctgtg
     8581 gacgctcttg acgagctcct tgcgacgatc ctcggtcaac ggcgggatgt tgaggcggac
     8641 gaccgtcccg tcgacgttcg gcatcagccc gatgtcgctc ttctggattg ccttctcgat
     8701 ggcgccgagc acgccccgat cccacggctg gatgacgatc tggtgggcct ccgggacgct
     8761 gatcccggcc agctggttga gcggggtctg ggtcccgtag tagtcgacgg tgaggcgctc
     8821 gaccagcgca gtcgaggcgc gtccggtccg gaacgcctgg aagtcgcgct ccatggcgtc
     8881 cacggcgcgg ttcatcttgt gatcggcgga cgagatcgtc tcgctgctca tcgggtacct
     8941 ccgcgggcgc ccgcggcgcc ggagatccgg gttccgaccg gctcgccggt ggccaccctg
     9001 gtgatgttgt caggctggtt gagatcgaac acgacgatcg gcagatcgtt ctccatgcag
     9061 agcgagaccg ccgccgcatc gaggaccttg agctggtcgc ggagcaggtc cgcgtactcg
     9121 agctcagcgt agcgccgcgc gttcggatgc gtctggggat cggcgtcgta gacaccgtcg
     9181 accttcgtgg ccttgagaag gacctccgcc ccgatctcga cggcgcgcag cgcggccgcg
     9241 gtatcggtcg tgaaataggg gttgccggtg ccggccacga acagggcgac ccggcccttc
     9301 tcgaggtggc ggacggcgcg ccgccggatg tacggctcgg cgacctcgtt catcgcgatc
     9361 gcgctcatca cccgggtcgg gacgccggcc cgttcgagcg cgtcctggag ggccaggccg
     9421 ttcatcacgg tcgccagcat gccgatgtag tcgcccgtcg cgcggtccat cccgctcgcc
     9481 gcggccgcga gtccgcggaa gatattgccg ccgccgacca cgatcccgac ctggacgccg
     9541 agccggtgga cctcggcgac ctgacgggcg atgaacgcgc agaacgcggg gtcgacgccg
     9601 taggaacggt tgccgagcag cgcctcgccc gacagcttga ggaggatccg cgagtagcgg
     9661 accttctccc ctgccgcggg ggacgtcgcc gtggtcgctc cgggggcgtc gctcacagtt
     9721 cttccccgag cgcgtagcga acgaagcggc gaacgcggat gttctcgccg atcgtggaga
     9781 tcttttccgt gaccaggtct cgcaccaagc gctcctcgtc gcggaagggc tgatcgtaca
     9841 ggcagacctg cgagaaccac ttcttgagct ggccctcgac gatctggggc cggatggcct
     9901 cgggcttctt ctgggtggcc tcgtccgaga ggagctcgga cttcctggcc tcgaggacgt
     9961 cggccgggat gcggtcggca tcgacgtaca ggggctgcat cccggcgacc tggatcgcga
    10021 ggtcgcggac gagcttctgg aactcgtcgg tgcgagcgac gaagtccgtc tcgcagttga
    10081 cctcgatcag gacgccgacg cggccgccgg tgtggatgta gctggaaacg aggccctcgc
    10141 gggcgtcgcg gccggccttc ttcgtggccg cggccaagcc cttctcacgg agcagggcaa
    10201 cggccttctc gacgtcgccg tcgctcgcga cgagggcgtt cttgcaatcc atgaagccgg
    10261 caccggtccg ctcccggagg tccttgaccg ctgccgcgga gatatctgcc atgtggtggg
    10321 gtcgctcctg gttcgtggtg gcggcgggca cggggcccgc ggtcggtagc ggtggtcgat
    10381 cggggaacaa cctcacccga tcgggtgact ggcgtcgtac gcccggccgg taagccgggc
    10441 ccacgtcgcc cggttcaacc cgcgctggcg ggctcttcct tctcggctgc ttcccgggcg
    10501 agctcggcgg cgatctcctc ggggctggcc tcggcggccg gagccgtctc ggcgaccggg
    10561 gcgaccggac gcgcacccgg caggagctcg tcctcgtcgg cgtcgggctc gaaggacagg
    10621 gccgcgccac cggcgagggc cgcgaccagc tcgttcgacg cggactcgtc gtactccggc
    10681 tcggtcgcca tcgcgacctc cggctcgggc tcgctggcac gcgcggcgcg ctcctgctga
    10741 ccctcgatgc aggcgtcggc gatcagggtg cacagcagcc ggatcgcgcg gatcgcgtcg
    10801 tcattggccg ggatgatgtg atcgatctcg tcgggatcga cgttcgtgtc gccggtcgcg
    10861 acgacgggca gctcgagctt gttcgcctcg gtgaccgcga tccgctcgcg gtgcgggtcg
    10921 atgatgaaga tcgcgccggg gacgcgcttc atcttgcgca tcccgccgac cgtcagggtc
    10981 agcttgttca tctcgtcggt gagcttggcc gcttccttct tcgtcatccg ctcgaagtcg
    11041 ccggcctgct gacgggcctc gagctgctcg agcagcgaga tccgcttctt gatcgtgctg
    11101 aagttggtca gcatgccgcc gagccagcgc ttgttgacga acggctggcc ggcccgggtc
    11161 gcctcctggg cgatcggctc ctgggcctgc ttcttcgtgc cgacgaagag gacgtactcg
    11221 ccatgggcga cggtctcgcg gacgaaggcg agcgacgact cgaggtgctt gaccgtctgg
    11281 gcaaggtcga tgatgtggat gccgttgcgc tcggcaaaga tgaagggctt catcttcggg
    11341 ttccagcggc gggtctgatg cccgaagtgg acaccggcct cgagcagctg tcgcatggaa
    11401 acgttcggca cggggagagg cctcctgtcg gtcttgttcc tccgcggacc tcgtcgccgg
    11461 gtctccggtc ctggtcgccg ccgcgttgcc gcggtcgtcg tccggtccgg caccgtcccg
    11521 gtcggaacgg tccgcgtgtg atctccgtcg ccgtgcggca gtggccggcg gcgcgggtgg
    11581 gtgagcacgg tcccggcggt cccggggccg cgcggagtgt agcacggggc cgattccggg
    11641 ccggtcgggg cggcctgacg cgtgcgcggg gcgcgcgcca ccgttgatgc accagctgac
    11701 tcagatcggc agtcgcacgc tcggccgaac gcggatcgcg acccagccat tcacgccgag
    11761 cccgaatctg atgcagcagg tgacccaggg tgccccggac ctcgtcagag cgggatcgcg
    11821 cgatggtggc ggatccgtgc cgactcaccc ggcgcgatcg gtggctcgac gacgacgagg
    11881 tccacccgga tcggcagcgg cggcagcctg ccgacgttcg gcagcccgtc ctcgagcagg
    11941 cggccaatcg ccgcccggag gtgggcgcgt ttgcgatggt cgatgctctc ttcagccagt
    12001 ccgaaggcgc gcgaccgacg ccagcgaacc tcgacgacca cgagctcggc gggcggtcca
    12061 gggtcgacgg ccacgatatc gagctcgccc cggccgaccc tcaggttgcg ggccatgatc
    12121 gtccagccct gacgagcgag accagcggcg acctccgact ccgcatcgtc gccgctggcc
    12181 tgggcgtcgg tccgcatcga gcgcacccta cgcccgaccg atcaccccgg acttatccgg
    12241 cgaccggctc cagcggcgca tcgtcgaagg caaccatgtc cagtgccgcc agatcggccg
    12301 cgtcgagcat cgcgtcgtcg gccatgacgg acacggactc acgatccagc agctcgtggg
    12361 tgtcgggcga ggcgcccggg tcgccgagaa gatcgaagtc gagctgatcg ccggcgagcg
    12421 tccgctgcag ggcgaggaac gagcggcggt ggaacggggt caggcccatc gcccggatcg
    12481 cgaggcggtg gtcgagggtg gcgtacccct ggttgtgctc ccagccgtaa cccgggtagc
    12541 gggcggcgag caggctcatc atccgatcgc ggacgacctt cgcgaccacc gatgcgcagg
    12601 cgatcgaata gaccttggcg tcaccgtcga cgatgttcgt gtacggcccg acgagggcct
    12661 cgaagcccgc gatccggttg ccgtcgacca tgacgtgctc gtgcccgccg agccgggcga
    12721 tcgcccggcg catcgcgaga tgagtcgcgt ggtagatgtt cagctggtcg atctcgcgaa
    12781 ccgaggcggc accggccccg accgcgacgg cccgcgccaa cctggcgctg ggtcggtcgg
    12841 cttgagtccc ggggactgaa ttcacgagcc agcgagcgag acaccaaccc gagatcgacg
    12901 gaaccgacgt ccggccaagc gtgccatacg gtcaggcgag tctc
//
LOCUS       DATAIW010000265         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_75746, whole genome shotgun sequence.
ACCESSION   DATAIW010000265 DATAIW010000000
VERSION     DATAIW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75746"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHM48_08430"
     CDS             <1..376
                     /locus_tag="VHM48_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755475.1"
                     /translation="PYLSYAAKIEPNPEAGTVRVNRISPTGFEILEAPMPALVVATQA
                     LGEPRYPSLKGIMAARSKTLETKAVADVAVGDAAVGGAVATTAVLGSEAPPPRAATRV
                     IREPAEEAARQVVDFLAQRRII"
     gene            376..1359
                     /locus_tag="VHM48_08435"
     CDS             376..1359
                     /locus_tag="VHM48_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2755476.1"
                     /translation="MGAASPIWVHGEIAADGTLAKISTEVATLARALGEAGGRDVVGV
                     VAGADPTAAATELAAFVPRVLAVTAPATADNAAGAIVGQRLTALIERDQPAFLLTGAG
                     PEGRDVAGVVSALTGWGVLVNAVGVAWDDGPVVSMSVFGGRLTTTSRLTGDQGIITVR
                     PNVVTAEPAASTGTVEATTVDGALALPLVRILDRVEAAGVAAPIEEARIIVAGGRGVG
                     GPDGFKVVEELAAALGGAVGATRAAVDSGWIPYAQQIGQTGKIVKPALYLALGISGAI
                     QHKVGMQTAGTIVAVNRDPDAPIAEFADVVVIGDLFEVAPALLAALRARAG"
     gene            1409..1960
                     /locus_tag="VHM48_08440"
     CDS             1409..1960
                     /locus_tag="VHM48_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755477.1"
                     /translation="MQPLLVLPLLAFLILLTLFLLILRRAGRFVTATRDAERFRRQAG
                     DLAGRVEVSLGEICGRIDAVRRGQLGADQVADDLTASLDAVRRYAEEARGFHPPADGI
                     AIRNEIVAALERSARALEMVEHGRSIQASARSGGREIEAQTAIKRGYLNVLHARESIA
                     HEAKAARDLSATDEASRFQRRNA"
     gene            2042..2527
                     /gene="nrdR"
                     /locus_tag="VHM48_08445"
     CDS             2042..2527
                     /gene="nrdR"
                     /locus_tag="VHM48_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX2755478.1"
                     /translation="MRCPHCGERETRVVDSRDLDDSATIRRRRECSACATRFTTYERV
                     EAARLVIVKRDGGRQEFDREKLASGLRKALTRRPVADDAADKAADEIEAELRSQGTTE
                     VDSSRVGALAMAKLRELDQIAYIRFASVYQSFDDLEALKREVDSLFEERGPETFGKGG
                     K"
     gene            2524..3132
                     /gene="dcd"
                     /locus_tag="VHM48_08450"
     CDS             2524..3132
                     /gene="dcd"
                     /locus_tag="VHM48_08450"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX2755479.1"
                     /translation="MSVLSDRDIRAALEAGDVAIRPYDAADLQPSSVDLHLDRSFRVF
                     RNNRYPYIDVRAPQPDLTELLTINDDEPFVLHPGEFVLGQTLEWVELPNDLVARLEGK
                     SSLGRLGLLIHSTAGYVDPGWKGNLTLELSNVANLPIALYFGMKIGQISFFKMSSPVE
                     RPYGSPSLGSKYQGQSEPTASAFHRDFTGPRVPSATETERRD"
     gene            3303..3983
                     /locus_tag="VHM48_08455"
     CDS             3303..3983
                     /locus_tag="VHM48_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2755480.1"
                     /translation="MNCLLIETPAGRVLVETGIGDRLDHKNRAMRGYEGPWIVPALRT
                     AGFDPTSVDVVALTHLHFDHAGGLLAGDGSRAFPNASIVAQRAEWEIALGDNPRLVAS
                     YVQPELQLVRDWGAEGWADGERELLPGVSVLPTGGHSAGHQAIVVRAPGAAPLAFFGD
                     LAMRPWSANPRWVTAFDDFPLDSVVVKGSLFARAADEGWTVVLSHEKAHPVGRLVRDR
                     DRFLYEAE"
     gene            complement(4344..5105)
                     /locus_tag="VHM48_08460"
     CDS             complement(4344..5105)
                     /locus_tag="VHM48_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2755481.1"
                     /translation="MTARPEFTHRPPKRGLIGPFGGRQLLAGAMVILVVGVLLVVVTT
                     PLGSATPVGPNDPQATQYVLDPNARIGVRPGQIAPELEAVRADGSTFQLTDILGNPVR
                     LADLRGKAVWINFFASWCPPCQAETPILRDLADRYRNRGLVVIGISVQETSAANVAAY
                     AAKYQLGYTIAADVTGEIYALYRPPGLPTQIFIGPDGAVRSFVLAPLREAAAVAQVEA
                     ILPSPSQPTLASSVPAPSGNPGASTSPSASRSPAP"
     gene            complement(5112..>5568)
                     /locus_tag="VHM48_08465"
     CDS             complement(5112..>5568)
                     /locus_tag="VHM48_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2755482.1"
                     /translation="LDAGASASVATTTGSMSFAPAGGPGFGDRLGGRIVSSRGGWLAS
                     FGLGAIFAIGWTPCIGVILGGILGLASASATAGQGVILLLAYTAGLGLPFIAIALVYD
                     RAPALLRPLVRHGRAVSIVGGLLVVAIGLAMIFDWLRLLPQYVPFNSAI"
BASE COUNT          812 a         1992 c         1964 g          800 t
ORIGIN      
        1 gccgtacctg tcctacgccg cgaaaatcga gccgaacccc gaggccggta cggtccgcgt
       61 caaccggatc agcccgaccg gcttcgagat cctcgaggca ccgatgcccg ccctcgtcgt
      121 cgcgacgcag gccctcggcg agccgcgtta cccgtcgttg aagggcatca tggccgcccg
      181 ctcgaagacc ctcgagacga aggccgtcgc cgatgtcgcc gtgggcgatg ccgcggtcgg
      241 cggcgctgtc gcgacgaccg ccgtcctcgg gtcggaagcg ccgccgcccc gcgctgcgac
      301 ccgggtgatc cgcgaaccgg ccgaggaggc cgcccgccag gtcgtcgact tcctcgctca
      361 gcggaggatc atctgatggg ggcggcttcg ccgatctggg tccatggcga gatcgccgcg
      421 gatggaaccc tggcgaagat cagcaccgag gtcgcgacgc tggcgcgggc gctcggcgag
      481 gccggcggcc gggacgtcgt cggtgtcgtg gccggcgccg atccgaccgc cgcggccacg
      541 gagctcgccg cgttcgtccc gcgcgtcctc gcggtcacgg cgcccgccac cgccgacaac
      601 gcggcgggcg cgatcgtcgg ccaacgcctg accgccttga tcgaacgtga ccagccggcg
      661 ttcctgctga ccggggccgg acccgagggc cgcgacgtcg cgggcgtggt ctcggccctg
      721 accggttggg gcgtgctcgt caatgcggtc ggcgtggcgt gggatgacgg tccggtcgtg
      781 tcgatgagcg tgttcggcgg ccggctcacg acgacgagcc gtctcacggg cgaccagggc
      841 atcatcaccg tccggccaaa cgtcgtgacc gccgagccgg cggcctcgac aggcacggtc
      901 gaggcgacga ccgtcgacgg cgctctcgcg ctgccgctgg tccggattct cgatcgggtc
      961 gaggccgccg gcgtcgccgc cccgatcgag gaggcacgga tcatcgtcgc cggaggccgg
     1021 ggggtgggtg gtccggacgg cttcaaggtc gtcgaggagc tcgccgcggc gctcggcggt
     1081 gccgtcggcg cgacccgcgc ggccgtcgac tccggctgga tcccgtacgc ccagcagatc
     1141 ggccagaccg gcaagatcgt caagcccgcc ctgtacctcg cgctcggcat ctccggggca
     1201 attcagcaca aggtcggcat gcagacggcc ggcacgatcg tcgccgtcaa ccgcgacccg
     1261 gacgccccga tcgccgagtt cgccgatgtc gtcgtgatcg gcgacctgtt cgaagtcgct
     1321 cccgccctcc tcgccgccct ccgcgcccgg gccggctgag gcggcgggca aggcgagccg
     1381 ggtcgaccgg ggccggacgg acgacgacgt gcaacctctg ctcgtcctgc cgctgctcgc
     1441 attcctcatc ctgctgaccc tctttctcct catccttcgg cgggccggcc gcttcgtcac
     1501 cgcgacccgc gacgcggaac gcttccgtcg ccaggccggc gatctggccg gccgggtcga
     1561 ggtgtccctc ggcgagatct gcggtcggat cgacgccgtg cggcgcggcc agctcggggc
     1621 ggaccaggtg gcggacgacc tgaccgcatc actcgacgcg gtcaggcgtt acgccgagga
     1681 ggcgcgcggc ttccatccgc cggccgacgg catcgcgatc cgcaacgaga tcgtggcggc
     1741 gctcgaacgg tctgcccggg cgctggagat ggtcgagcat ggccggtcga tccaggcgtc
     1801 ggcccggtcg ggcggtcgcg agatcgaggc tcagaccgcg atcaagcggg gttacctcaa
     1861 cgtgctccac gcccgcgaat cgatcgccca cgaagcaaag gcggcgaggg acctctcggc
     1921 gacggacgaa gcgagccgct tccagcgccg caacgcctga atcgcgcccg gacacccgct
     1981 atgtagtggt atcatcggcg gacaccacaa gtggtagtgg tgcgagccat caggagcccg
     2041 gatgcgttgt cctcattgcg gtgagcgcga gacgcgcgtc gtcgactcgc gcgacctcga
     2101 cgactcagcg acgatccgcc gtcgccgcga gtgctccgcc tgcgcgaccc ggttcacgac
     2161 ctacgagcgg gtcgaagccg ctcgcctcgt catcgtcaag cgcgacggcg gtcgccagga
     2221 gttcgatcgc gagaagctcg cctccggcct gcgcaaggcc ctgacccggc gtccggtcgc
     2281 cgacgacgcc gccgacaagg ccgccgacga gatcgaggcc gagcttcgtt cccaggggac
     2341 caccgaggtc gactcgtcgc gcgtcggtgc gctcgcgatg gcgaagctcc gcgagctcga
     2401 ccagatcgcc tacatccggt ttgccagcgt ctaccagagc ttcgacgacc tcgaggcgct
     2461 caaacgcgag gtcgacagcc tgttcgagga gcgcggcccg gaaaccttcg ggaagggcgg
     2521 caagtgagcg tcctctccga ccgcgacatc cgcgccgcgc tcgaagccgg tgacgtcgcc
     2581 atccggccgt acgatgccgc cgatcttcaa ccctcgtcgg tcgatcttca cctcgatcga
     2641 tcgttccggg tcttccgcaa caaccgctat ccgtacatcg acgtccgcgc tccgcagccg
     2701 gacctgaccg agctccttac gatcaacgac gacgagccct tcgtcctcca tccgggcgag
     2761 ttcgtccttg gccagaccct cgagtgggtc gagctcccga acgacctcgt ggccaggctc
     2821 gagggaaagt cgagccttgg ccgtctcggc ctcctgatcc actcgacggc gggctatgtc
     2881 gatccgggct ggaagggcaa tctgacgctc gagttgtcga acgtcgccaa cctcccgatc
     2941 gcgctgtact tcgggatgaa gatcggccag atctcgttct tcaagatgag ctcgccggtg
     3001 gagcggccgt atggctcgcc gtcgctcggg tccaagtacc agggccagtc agagccgact
     3061 gcatcggcgt tccaccgcga cttcaccggg ccgcgcgtgc cgtccgcgac ggagaccgag
     3121 cgacgggact gaacgacgcg gtcgactggc ggcgcgacct cggcggcggg atctcggtcg
     3181 ccgtcatcca ggccgggacg ttccgctccg acgcgggcgc cctcttcggg ccggtcccgc
     3241 gcctcctgtg ggatcgctgg gcgcgcgacg agctcgaccc ggagaaccgc ctcctccagg
     3301 cgttgaactg tctgctgatc gagacgccgg ccgggcgcgt cctcgtcgaa accgggatcg
     3361 gcgatcgcct cgaccacaag aaccgggcga tgcgcggcta cgagggcccg tggatcgtcc
     3421 cggctctgcg gaccgccggg ttcgacccga cctcggtcga tgtcgtcgca ctgacccacc
     3481 tccatttcga ccacgccggc ggactcctcg cgggcgacgg ctcgcgggcc ttcccgaacg
     3541 cctcgatcgt cgctcagcgg gccgaatggg agatcgccct cggcgacaat ccacgcctcg
     3601 tcgccagcta tgtccagccc gagctccagc tcgtccgcga ctggggagcg gagggctggg
     3661 ccgacggcga gcgtgagctg ctgccgggcg tctcggtcct cccgaccggc ggtcattcgg
     3721 ccggccacca ggcgatcgtc gtccgcgcgc cgggtgccgc gccgctggcg ttcttcggcg
     3781 accttgcgat gcggccgtgg agcgccaacc cgcgctgggt cacggcgttc gacgacttcc
     3841 cgctcgactc cgtggtcgtg aagggctcgc tcttcgcccg cgccgccgac gaaggctgga
     3901 ccgtggtgct gtcgcacgag aaggcacatc cggtcggacg cctcgtccgc gaccgggatc
     3961 gcttcctcta cgaggcggag tagggaccgt ctgctcgacg ccgcggcctc cgttcggcgc
     4021 gtcaaaggac gtatcccggc ggccgtcatg agtcctggac ggttgatgac ttccggcagg
     4081 tatcaaggcg ccctgcctcg gcctaggttg tgctcagaca gacggaccgc cggacaacga
     4141 aacctgagtc cggtgccgcg tagtccagac ggcgggacgc cgaacggcta cggagccggc
     4201 attgatgccg cctcggcggt ccgtcttctt cctgtccgcg acgacctcag ccggccgcct
     4261 cgtgcgcgcc ggcttcgctg tgttcctggg ctgccggtgc gactccgggt cgagaaccgt
     4321 cgtcgcccga ctcgacagtt cgactacggc gcgggtgagc gcgatgccga cggagacgtc
     4381 gacgcgccgg ggttgcccga tggcgccggc acgctcgacg cgagggtcgg ttgggacggt
     4441 gacggcagga tcgcctcgac ctgggcgacc gccgcggcct cgcgcaacgg cgcgaggacg
     4501 aatgagcgga ccgctccgtc ggggccgatg aagatctggg tcggcaggcc gggcggccgg
     4561 tacagggcgt agatctcgcc cgtgacgtcg gccgcgatcg tgtagccgag ctggtatttc
     4621 gcagcgtagg cggcaacgtt cgccgcgctc gtctcctgga cgctgatccc gatcacgacg
     4681 agaccgcggt tccggtagcg gtcggcgagg tcccgcagga tcggcgtctc cgcctggcac
     4741 ggcggacacc atgacgcgaa gaagttgatc cagaccgcct tcccgcggag gtcggcgagg
     4801 cggaccgggt tgccgaggat gtccgtcagc tggaacgtgc tgccgtcggc tcgcacggcc
     4861 tcgagctccg gggcgatctg gcccggccgg acgccgatcc gggcgttcgg atcgaggacg
     4921 tactgggtcg cctgcggatc gttgggtccg actggagtcg ccgacccgag cggcgtcgtg
     4981 acgacgacca ggagaacgcc gacgacgagg atgaccatgg ccccggccag tagctggcgc
     5041 ccgccgaacg gcccgatcag cccgcgcttc ggcgggcgat gggtgaactc cggacgagcg
     5101 gtcacgaacg gtcagatcgc gctgttgaac ggaacgtact ggggcagcag gcggagccag
     5161 tcgaagatca tcgccaggcc gatcgcgacc acgagcaggc caccgacgat cgacacggcg
     5221 cggccgtggc ggaccagcgg ccgcaggagg gccggcgcgc ggtcatagac gagggcgatc
     5281 gcgatgaacg gcagcccgag cccggccgtg taggcgagga ggaggatcac gccctggccg
     5341 gcggtcgccg acgccgaggc cagccccagg atgccgccca ggatcacccc gatgcacggc
     5401 gtccagccga tcgcgaagat cgccccgagc ccgaagctgg cgagccagcc gccgcggctg
     5461 ctgacgatcc ggccaccgag ccggtcgccg aagcccggtc caccggccgg agcgaaggac
     5521 atcgagcccg tcgtcgtcgc gaccgaggcg gacgccccgg catcgagc
//
LOCUS       DATAIW010000266         9750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_45827, whole genome shotgun sequence.
ACCESSION   DATAIW010000266 DATAIW010000000
VERSION     DATAIW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45827"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VHM48_08470"
     CDS             complement(<1..754)
                     /locus_tag="VHM48_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX2755483.1"
                     /translation="MQLAFLGLGQIGGSIARAASVAGFVSRVSAWTPSGTAPRAARAD
                     GIVAAATVAEAIRDADLVVLAAPPLACLELIDELAGVLAGDLARDAVITDVASTKAAI
                     VERARSHGLRFVGGHPMAGRETAGYAAADPDLVRDRPWVIVNADPPDAAADARVTALA
                     TACGARPMVLTAEDHDAAVAAISHLPLVISAALVEAMTAQPEWPVARDLAAGGWAGMT
                     RLARGDPTMGAGILATNGAATATRLRALRTVID"
     gene            831..1520
                     /locus_tag="VHM48_08475"
     CDS             831..1520
                     /locus_tag="VHM48_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755484.1"
                     /translation="MTSEPKRIRVLFVEDHRLLADALSALVAREPDIEVVGVAGTVAD
                     AKLMARERLDVVLMDYHLPDGTGAEATRAIKARWPAARIVMVTALSDDETVLESIQAG
                     ADGYLTKDRAAEDVVQAIRAAHAGETLLPRSVIVEIARRVAAARERGDERRIAEPLTP
                     RELEVLRALTEGLSTPDICDQLFIAPNTLRTHVQNIMGKLRVHSKLEAVAFALRYRLV
                     EPPRPDDAVPT"
     gene            complement(1563..1811)
                     /locus_tag="VHM48_08480"
     CDS             complement(1563..1811)
                     /locus_tag="VHM48_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755485.1"
                     /translation="MPKLACRSCGRQIYTVAPIESLFAEERRCPRCGAFLNDERRDVE
                     RRGYVRRQNPADDPGPPKTKERRAEERRQARRRKPAGS"
     gene            complement(2048..2575)
                     /locus_tag="VHM48_08485"
     CDS             complement(2048..2575)
                     /locus_tag="VHM48_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755486.1"
                     /translation="MPTEGPIVAEIPTPTPGATAPDQSPIVATPRPTVHPTPRPTVNP
                     TPTPTVHPTPRPTVNPTPTPTVQPTPRPTVHPTPTPEPVVGKVHKPRPPCPGDTSGPP
                     GHNKVSPPPARPCTPVAHGKAAGSNGMVVVLPLALGGLAAAIRTRFVLASRRLVRASR
                     SRTGRRRDRRVHGQA"
     gene            complement(3020..3598)
                     /gene="thpR"
                     /locus_tag="VHM48_08490"
     CDS             complement(3020..3598)
                     /gene="thpR"
                     /locus_tag="VHM48_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2755487.1"
                     /translation="MNEEPRLFVAVPLSESARAAVAEVVERIRADEPEGRGVRWVRLD
                     GLHITLRFLGPTPESRVADVAAAVATTAAGVAPFAIRVAGADAFPPVGRPRTLWLDLD
                     RGVDDLAGLAARLDDALADLGWERERRPFRAHLTLARADGVRAGPATVAALRAAAAEL
                     AIESPIERVVLFESITGSGRARYIDRADVALG"
     gene            complement(3595..4128)
                     /locus_tag="VHM48_08495"
     CDS             complement(3595..4128)
                     /locus_tag="VHM48_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX2755488.1"
                     /translation="MSPSANDFPSVVEVVVEIPRGSRNKYEYDEAADVFRLDRVLASA
                     VHYNFDYGFITDTRAGDGDHTDALLLIDEPTFPGCRVWARPIGGLEMRDDKGEDFKVL
                     CVAVADPHQAHVEDLEHVRPHRLVEIEHFFDTYKMLEDKETDVVGWRDAARARRVLEA
                     DRATWRAERTAASDRHA"
     gene            complement(4590..6101)
                     /locus_tag="VHM48_08500"
     CDS             complement(4590..6101)
                     /locus_tag="VHM48_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755489.1"
                     /translation="MGEPQFAGVFGSVVARPVDRRLARLLVLLLAGLAVAGTSPSPPP
                     PPLATAHAEGELHLTAERPIASVDVTIQANAVLRGLKGSVVTGQVASDPVGSAPAGPA
                     IVTIVPLDDPASSVSLGSAPSVSVRLDAGCPPDDACARHYRITAVLVDPTAEDAVVSW
                     HASAEFRSDGPERPSAAPADGRLEVAADDVTTRPAVELARASLAPETIHLDRDHPLAS
                     RDLEIDQPPGLAGAGAERTWLAFLTVTAPGAPAVAGDRPAIVQVGMPGREQPLASGSI
                     GRHPIYPDRCAGTTGCPTPLRLDAILPGGPGDLAKDVTWSIDLVAYGPVGAAPPEPLA
                     ARLLGSTAIDPSAPRLTASETGAFDIAPRVPGRRNATVTLDATRLAQVDGPISGQLQL
                     RFEVTTTSTGPVQTLDLALGAGSSAGGLGAAYESVPSGRVDDLDSAVVAVECDGRSSC
                     QVDIPFSVATQVDATANVHVAWKLTALWYPDAPHRLPPDVALLLAIETASPRP"
     gene            complement(6205..7278)
                     /locus_tag="VHM48_08505"
     CDS             complement(6205..7278)
                     /locus_tag="VHM48_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2755490.1"
                     /translation="MTDTAEPTAAAQSWRLDADTGSMVPRSALDVLVDLNDKVTTGRV
                     SEYQPVPLGFTPLDKTIGTGLRAGELLLIGGAQGTGKTTMALQMARNIASGGQANVLY
                     ICFEHDEQYLLNRMIAMESALAHLPHKTGAIKIQDVRKEILGTWMAEGGGVAQLASNP
                     RLRPSLDRIARYGQNLFLLRGSQTASTVDNIRKLVQEHRRLSGDRRLVVFVDYLQKVP
                     QLPEPNSETEKVTYVVNGLKDVALSEDVPMIAIVAADKEGLKASRLRNYHLRGSSAIN
                     YEADIILILNEKYHIVAKVNIEFNPYQAQRFRDWVVISVEKNRGGQDNVDLEFEKHFE
                     FSCFDPNGRTVQEKLIEERLYND"
     gene            complement(7421..8830)
                     /locus_tag="VHM48_08510"
     CDS             complement(7421..8830)
                     /locus_tag="VHM48_08510"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2755491.1"
                     /translation="MTMTTATDQALEDFLAAHAAERLESYKEFVRIPSISALPAHAPD
                     CRRAAEWIAADLERIGMEHVEVAETGGHPLVYADWLHADDAPTVLVYCHYDVQPVDPL
                     ELWTSPPFEPVVVGNRILGRGVADDKGQLHLHLRAAEALLATRGRLAVNLRFVFEGEE
                     ESSSVNLDAWLTANRDRLTADFALISDTGFFEGNIPSITIALRGMMYAQVDVTGTAVD
                     LHSGGYGGTVENPANALARIIAGLKDEDGRILVPGFYDDVVELTTDERAMVDALPFDE
                     ADYLARLGLPELHGERGYSTLERRGSRPTLDVNGLWGGFQGEGSKTIIPAHAHAKISC
                     RLVTAQDPDRIFAAFRDHILAMAPAGVRVDVRQLGGGRPSLTPIDHPATQAAARALEA
                     TFGQAPVYIREGGSIPVCATFEATLDLPVVLLGFDPPDQRAHAPNEWMDLDNYETGIR
                     TIARMWDEVAAVGTVGLRS"
     gene            complement(8959..>9750)
                     /locus_tag="VHM48_08515"
     CDS             complement(8959..>9750)
                     /locus_tag="VHM48_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755492.1"
                     /translation="LVNEAAILAARRNKKVIGITEFTEALERIVAGPERKSRIISDAE
                     KAIIAIHEGGHAVVQRILPKCDPVAKVTIISRGMALGYTMALPMEDRYLQSKTEFEDK
                     IAGLLGGNAAERLVFGDTTTGASNDIEKATDLARRMVTEFGMSDKLGPLSFGKRDEMI
                     FLGRSMGEQRDYSDEVAKTIDEEVRAIIDHAYERAMDVLVTHRDKLNALAEKLVAEET
                     VDGDGFEALFSDLPPKPNSRETPPTVVGPNQPTPGTLPAPNPAPA"
BASE COUNT         1399 a         3344 c         3564 g         1443 t
ORIGIN      
        1 cgtcgatcac cgtccggagc gcccgcaacc gcgtcgccgt cgccgccccg ttcgtggcga
       61 ggatcccggc gcccatcgtc gggtcgccgc gagcgaggcg ggtcatcccc gcccaacccc
      121 cggcggcgag gtcccgagcg accggccact ccggctgcgc ggtcatcgcc tcgacgaggg
      181 cggccgagat gacgagcggc aggtggctga tcgccgccac cgcggcatcg tggtcctctg
      241 ctgtcaggac catcggacgg gcgccacacg cggtcgcgag cgcggtcacc cgggcatcgg
      301 cggctgcgtc cggtgggtcc gcgttcacga tcacccacgg ccggtcgcgc acgaggtccg
      361 gatccgcggc cgcgtagccc gcggtctcgc gaccggccat cgggtgccca ccgacgaacc
      421 gcaacccgtg cgaacgggct cgctcgacga tcgcggcttt cgtgctcgcc acatcggtga
      481 tcactgcatc gcgggcgaga tcgccggcca gcaccccggc cagctcgtcg atcagctcaa
      541 ggcaggccag cggtggcgcg gccagcacga ccaggtcggc gtcgcggatt gcctccgcca
      601 ccgtcgcggc ggcaacgatc ccgtcggccc gagcagcgcg cggcgcggtc ccggacggcg
      661 tccaggcgga gacgcggctc acgaatccgg cgaccgacgc cgcgcgagcg atcgacccac
      721 cgatctgacc caggcccagg aatgcgagtt gcacgccgcg agtatgtcag tccgatcgac
      781 gacgctggta tcctcgacga gacgcatgcc gcaagtcgct ggagggaatc gtgacttcgg
      841 agcccaagcg gatccgcgtc ctcttcgtgg aggaccaccg tctgttggcc gacgctctgt
      901 cggccctcgt cgcccgggag cccgacatcg aggtcgtcgg cgtggccggc accgttgcgg
      961 atgccaagct gatggcacgg gaacgactcg acgtcgtcct catggactac cacctcccgg
     1021 acggaaccgg cgcggaagca acccgcgcga tcaaggcgcg ctggccggct gcccggatcg
     1081 tgatggtcac cgccctcagc gacgatgaga ccgtcctcga atcgatccag gccggcgccg
     1141 acggctatct caccaaggac cgcgccgcgg aggacgtcgt ccaggccatc cgagccgccc
     1201 atgccggcga aacgctcctg cctcgctcgg tgatcgtcga gatcgcccgg cgcgtggcgg
     1261 cggcccgcga gcgtggcgac gaacggcgga tcgccgagcc actgaccccg cgcgagctgg
     1321 aggtcctccg cgccctgacc gaaggcctct cgacgccgga tatctgcgac cagttgttca
     1381 tcgccccgaa caccctgcgg acccacgtcc agaacatcat gggcaagctc cgggtccact
     1441 ccaagctcga ggcggtcgcg ttcgcgctcc gctatcggct ggtcgagccg ccccgacccg
     1501 acgacgccgt cccgacctga cgtgcgccgg accggacggg gccgcgccga ccctcgcgaa
     1561 cgtcaggaac cggcaggctt gcgccgtcgc gcctggcgcc gctcctccgc tcgacgttcc
     1621 ttcgtcttgg gcggtcccgg gtcgtcggcc gggttctggc ggcggacgta cccgcgccgc
     1681 tcgacatcac ggcgctcatc gttgaggaac gctccgcaac gcgggcaacg tcgctcctcg
     1741 gcgaagagcg actcgatcgg cgccacggtg tagatctggc ggccacacga tcggcaggca
     1801 agcttcggca tccgcgcatc gtagtcgacg cccgtcgccg cgggctgcga ccgccctgcc
     1861 tggccgaccc gttcaggggc ctgccgttta cgtcggatgg gggatgtgct cgcaggccgc
     1921 cgagacgaac aatccggctt gggttcgccc tcagggcgac cggccggtca ctcgggcagg
     1981 tggccgctca gcgggccgct tcgctccccc cggagcggcc cgctccccac ctccacggtt
     2041 ctcccggtca ggcttgaccg tggacgcggc ggtcgcgacg acgacccgtg cgcgaccttg
     2101 atgcgcgaac gagtcggcgc gaggcaagga cgaaccgggt ccggatcgca gccgccaggc
     2161 cgccgagggc gagcgggagg accacgacca tcccgttcga accggcggcc ttgccgtgcg
     2221 ccaccggcgt gcaagggcga gcgggtggtg gcgagacctt gttgtgaccg ggcggtccgc
     2281 tcgtgtcacc ggggcatggc ggccgtggct tgtggacctt gccgacgacc ggctccggcg
     2341 tgggcgtcgg atggacggtc ggacggggcg tcggctggac ggtcggagtg ggcgtcggat
     2401 tgacggtcgg acggggcgtc ggatggacgg tcggagtggg cgtcggattg acggtcggac
     2461 ggggcgtcgg atggacggtc ggacggggcg tcgcgacgat cggactctgg tccggggcgg
     2521 tcgcgccggg tgtcggggtc gggatctcgg cgacgatcgg gccctcggtc ggcacggcgg
     2581 acgatgcgga cggttgggcc gacggctcgg cggaggactg gtccgacggc tgggacgatg
     2641 gctcgacgac cgacgcggtg ggctcaggga cgaccgcgga gctcgtcggc gtcggctcga
     2701 cggctggaga cggagacgca gtcaccgcaa cgggtgacgg acgcggactg ccgacgatcc
     2761 cggccacttc gccgctgggt ccggcaggaa ccacgaaccc tgcagtcagc gcgacggtcg
     2821 cgaggatgag attcaggagc cccaaggcga cgaccaggcg tgcgcgctga gcggtggtca
     2881 cgacgtgttg tgggccccct ggcgttcgtc tccgggtctg cgctccggac cacccgatgg
     2941 tagcaccggc gaccttctct gcgcccggtc gggcgcccag cgcaacggat cagcgcgtcg
     3001 cgtccgaccc ggagcggcct cagcccagag cgacatcggc tcgatcgata tagcgcgccc
     3061 ggcccgatcc ggtgatgctc tcgaacagca cgacccgctc gatcggcgac tcgatcgcca
     3121 gctctgctgc ggccgcccgg agggccgcga cggtcgccgg tccggcgcgc acgccgtcgg
     3181 cccgtgccag cgtcaggtgg gcccgaaacg gacgccgctc ccgctcccag ccgaggtcgg
     3241 cgagggcgtc gtcgaggcgc gccgcgagcc cggccagatc gtcgacccca cggtccaggt
     3301 cgagccacag ggtccgcggc cgtccgaccg gcgggaaggc atcggcgccg gcgacccgga
     3361 tggcgaaggg ggcgaccccg gccgcggtgg tggccaccgc agcggccacg tccgcgacac
     3421 gggattccgg cgtcggcccg aggaaccgga gggtgatgtg gagcccgtcg aggcggaccc
     3481 agcggactcc ccgcccttcc ggctcatcgg cccggatgcg ctcgacgacc tcggcgacgg
     3541 cggcccgcgc cgattcgctc aacgggacgg cgacgaagag ccgcggttcc tcgttcaagc
     3601 gtggcggtcg gacgcggccg tccgctccgc acgccaggtc gcgcggtcgg cctccagcac
     3661 ccgacgggcg cgggcggcat cgcgccagcc gacgacgtcg gtctccttgt cctcgagcat
     3721 cttgtaggtg tcgaagaaat gctcgatctc gacgaggcga tgcggccgga cgtgctcgag
     3781 gtcctcgacg tgggcctggt gcggatccgc gaccgcgacg cacagcacct tgaagtcctc
     3841 gcccttgtcg tcgcgcatct ccaggccgcc gatcggtcgg gcccagacgc gacagccggg
     3901 gaaggtcggc tcgtcgatca ggaggagggc gtcggtgtgg tcgccgtcgc cggcccgcgt
     3961 gtcggtgatg aagccgtagt cgaagttgta gtggacggcc gaggcgagca cccgatcgag
     4021 acggaagacg tcggccgcct cgtcgtactc gtacttgttg cggctgccgc gggggatctc
     4081 gacgacgacc tcgaccacgc tcgggaagtc gttcgcgctg ggactcatcg gatcggcctg
     4141 ccacccatcg tcggagtgta gccgcggtgt ccgaaggctg cggttcgcgg ggctctcagc
     4201 ggtccccact cggccccgcg cggcgcgcac ggaaacgccc gaaggaaccg actgccaaac
     4261 gggttcgatc gtggaagcaa ccagccttga tgtgatcgcg gccggccagc tcccggaccg
     4321 gcggtcacgg agcgggcgcg ccttgcggct cgtccgaccg gaccggcggc gctgcgcggg
     4381 tcgcccctcg cctcgcctgt ccgtttgcgt tggccccttc gggcaagacg aaccatatgc
     4441 ccgggcccac ctggccacaa cgggcttatc cacgaacttc ggcggcccgc ccgcccagtc
     4501 atccacgaaa cagcgaggca tgttgacgaa atgtggaaac actggcgggg cggacgtgct
     4561 aggagaggct ctcgcgcgat cggcggcgat cagggccggg gcgatgcggt ctcgatcgcc
     4621 agcaggagtg ccacgtccgg cggcagccgg tgcggggcgt cggggtacca cagggcggtc
     4681 agtttccacg cgacatggac attggccgtc gcgtcgacct gcgttgccac gctgaacgga
     4741 atgtcgacct ggcagctcga gcgaccgtcg cactcgacgg cgacgacggc gctgtcgagg
     4801 tcgtcgaccc ggccgctcgg caccgattcg tacgccgcgc cgaggccacc ggcggaagag
     4861 ccagcgccga gcgcgaggtc gagcgtctgg accggtccgg tcgaggtcgt ggtgacctcg
     4921 aagcggagct gaagctggcc gctgatcggc ccgtcgacct gagcgagccg ggtcgcgtcg
     4981 agggtgaccg tcgcgttgcg tcggccgggc accctggggg cgatgtcgaa ggctccggtc
     5041 tcggacgccg tgagacgggg cgccgatggg tcgatcgccg tcgatcccag cagccgggcg
     5101 gccagcggct ccggcggcgc agcgccgacc ggtccgtagg caacgagatc gatcgaccac
     5161 gtcacgtcct tcgccaggtc gccgggtccg ccgggcagga tcgcgtcgag tcggagaggt
     5221 gtcggacagc cggtcgttcc tgcgcatctg tccggataga tcggatgtcg gccgatcgat
     5281 ccactcgcca acggctgctc gcgacccggc atgccgacct ggacaatcgc aggccgatcg
     5341 ccggcgaccg cgggcgcgcc gggcgcggtc acggtcagga acgccaacca ggtccgctcg
     5401 gcgccggccc ccgccagccc gggcggttga tcgatctcga ggtcgcggct cgcgagcgga
     5461 tgatcacgat cgagatggat cgtctccggc gccaggctcg cccgtgccag ctcgaccgcc
     5521 ggccgcgtgg tcacgtcgtc ggcggccacc tcgaggcgcc cgtcggcggg tgcggcagat
     5581 ggccgttccg ggccgtcgga ccggaactcc gcgctcgcgt gccaggacac gacggcgtcc
     5641 tcggccgtgg gatcgaccag gaccgcggtg atccggtagt gacgcgcgca ggcatcgtcg
     5701 ggcgggcagc cggcgtcgag gcggacggag accgacggag cggagccgag cgagaccgaa
     5761 gaggcgggat cgtcgagggg cacgatcgtg acgattgccg gcccggcggg cgcgctgccc
     5821 accggatccg acgcgacctg tccggtgacg accgagccct tgagcccgcg caggacggcg
     5881 ttggcctgga tcgtcacatc gaccgacgcg atgggccgct cggccgtgag atggagctcg
     5941 ccctcggcat gcgccgtcgc caggggcggt ggcggcgggc tgggcgaggt cccggcgacg
     6001 gcaagcccgg cgaggagcag gaccaggaga cgcgccaatc ggcgatccac cggtcgggca
     6061 acgaccgacc cgaagacccc ggcgaactgc ggctcgccca tcctcggcac ccccgcgccg
     6121 catcggcggc gctagtcgtt gtagagacgt tcctcgatcc tcgggcgaat ggcgtcgcgg
     6181 agctccgcct tcgacctcgg agagctaatc gttgtacaac cgctcttcga tcagtttttc
     6241 ctgaaccgtc cggccgttcg ggtcgaagca ggagaactcg aagtgcttct cgaactcgag
     6301 gtcgacgttg tcctggccac cgcggttctt ctcgaccgag atcacgaccc agtcgcggaa
     6361 gcgctgcgcc tggtacgggt tgaactcgat gttgaccttg gcgacgatgt ggtacttctc
     6421 gttgaggatg aggatgatgt cggcctcgta gttgatcgcc gacgagccac gcaggtggta
     6481 gttgcgcaga cgggaagcct tcaggccctc cttgtcggcg gccacgatgg cgatcatcgg
     6541 cacgtcttcg gacagggcga cgtccttgag cccgttgacg acgtacgtga ccttctcggt
     6601 ctcgctgttc ggctcgggca gctggggcac cttctggaga tagtcgacga agacgacgag
     6661 gcgccgatcg cccgacaggc ggcgatgctc ctggacgagc ttgcggatgt tgtcgacggt
     6721 gctcgcggtc tgcgagccgc ggagcaggaa caggttctgg ccgtaccgag cgatcctgtc
     6781 gagcgacggg cgaagccgcg ggttgcttgc gagctgggcc acgccgccac cctcggccat
     6841 ccacgtcccg aggatctcct tgcggacgtc ctggatcttg atcgcaccgg tcttgtgggg
     6901 caggtgggcg agggccgatt ccatcgcgat catgcgattg aggaggtact gctcgtcgtg
     6961 ctcgaagcag atgtacagga cgttggcctg gccaccggac gcgatgttgc gcgccatctg
     7021 gagcgccatc gtcgtcttgc ccgtgccctg ggcgccgccg atcaggagca gctcaccggc
     7081 ccggaggccg gtgccgatgg tcttgtcgag cggggtgaag ccgagcggaa cgggctggta
     7141 ctcgctcacc cgaccggtcg tcaccttgtc gttgaggtcg acgagcacat cgagcgccga
     7201 acgcggcacc atgctcccgg tgtcggcgtc gaggcgccag ctctgggccg cggctgtggg
     7261 ttcggcggtg tcggtcacgc tcgctcgtcc tcccggtcaa cgaattcggt gtgcatcgta
     7321 gcaccgcgtt cgagcccgac agccgcccga aagccgacgc cgggtgaccc gaaggtccta
     7381 tcgcggtgcg atctccacgt ggtctcgtcc gccacccgac tcagctgcgc agccccaccg
     7441 tgccgacggc ggcgacctcg tcccacatcc gggcgatggt ccggatgccg gtctcgtagt
     7501 tgtcgagatc catccactcg ttcggggcgt gggcacgctg atcgggcgga tcgaagccga
     7561 gcaggacgac cggcaggtcg agggtcgcct cgaacgtggc gcagaccggg atcgagccgc
     7621 cctcgcgaat gtagaccggg gcctggccga aggtcgcctc cagcgcccgg gccgccgcct
     7681 gggtcgcggg atgatcgatc ggggtcaggc tggggcgacc gccgccgagc tggcggacgt
     7741 cgacccggac gccggccggc gccatggcca ggatgtggtc gcggaacgcg gcgaagatcc
     7801 gatcgggatc ctgcgccgtc accagccggc agctgatctt ggcgtgggcg tgggccggga
     7861 tgatcgtctt gctgccctcg ccctggaagc cgccccagag gccgttgacg tcgagggtcg
     7921 ggcgggagcc gcgtcgctcc agggtcgagt agccgcgctc gccgtggagc tccggcaggc
     7981 cgagacgagc gaggtagtcg gcctcgtcga acggcagcgc gtcgaccatg gcccgttcgt
     8041 ccgtggtgag ctcgacgaca tcgtcgtaga agccggggac gaggatccgg ccgtcctcgt
     8101 ccttgagccc cgcgatgatc cgggcgaggg cgttggcagg gttctcgacc gtgccgccgt
     8161 agccgccgga atggaggtcg accgcggtcc cggtcacgtc gacctgggcg tacatcatcc
     8221 cgcgcagggc gatcgtgatc gacgggatgt tgccctcgaa gaagccggtg tcgctgatca
     8281 gggcgaagtc ggccgtgagg cggtcgcggt tggcggtcag ccacgcgtcg aggttgaccg
     8341 agctcgactc ctcctcgccc tcgaagacga acctgaggtt caccgcgagt cgcccgcggg
     8401 tcgcgagcaa cgcctcggcc gcgcgcaggt ggaggtggag ctggcccttg tcgtcggcga
     8461 cgccgcggcc gaggatccgg ttgcccacca cgaccggctc gaatggtggc gaggtccaga
     8521 gctcgagagg atcgacgggc tggacgtcgt aatggcagta gacgagcacg gtcggcgcgt
     8581 cgtcagcgtg aagccagtcg gcgtacacga gcggatggcc gccggtctcg gcaacctcga
     8641 cgtgctccat gccgatccgc tcgagatcgg ctgcgatcca ctccgcggca cgccggcaat
     8701 ccggggcgtg cgcggggagg gccgagatgc tcgggatccg gacgaattcc ttgtacgact
     8761 cgaggcgttc cgcggcatgc gccgcgagaa agtcctcgag ggcctggtcg gtggcggtcg
     8821 tcatcgtcat gacccgatgg tatcggctgg gccgcgatcg agccgggccg ccagcggggc
     8881 gcaacgccag cccgggaacg gacgaccccg ggcgttccgg cccggggtcg ccaatgtgcg
     8941 ggagaagccg tcggcggatc aggccggggc ggggttcggc gccggcagcg tgccgggcgt
     9001 cggctggttc ggaccgacca cggtcggcgg ggtctcgcgc gagttgggct tcggcggcag
     9061 gtccgagaag agcgcctcga agccgtcacc gtcgacggtc tcctcggcga cgagcttctc
     9121 ggccagcgcg ttgagcttgt cgcggtgggt gacgaggacg tccatcgccc gctcgtaggc
     9181 gtgatcgatg atcgcccgga cctcctcgtc gatcgtcttc gcgacctcgt cgctgtagtc
     9241 gcgctgctcg cccatcgagc gacccaggaa gatcatctcg tcgcgtttcc cgaacgagag
     9301 cggaccgagc ttgtcgctca tgccgaactc ggtgaccatc cgccgggcga ggtcggtcgc
     9361 cttctcgatg tcgttgctgg cgccggtcgt cgtgtcgccg aagaccaggc gctccgcggc
     9421 attgccgccg agcaggcccg cgatcttgtc ctcgaactcg gtcttcgact ggaggtagcg
     9481 atcctccatc gggagggcca tcgtgtagcc gagggccatg ccgcggctga tgatcgtgac
     9541 cttggcgacc gggtcgcact tcggcaggat ccgctggacg accgcgtggc ctccctcgtg
     9601 gatcgcgatg atcgcctttt cggcgtccga gatgatccgc gacttgcgct cgggaccggc
     9661 gacgatccgc tcgagcgcct cggtgaactc ggtgatcccg atcaccttct tgttgcggcg
     9721 cgcggccagg atggccgcct cattgacgag
//
LOCUS       DATAIW010000267         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_15934, whole genome shotgun sequence.
ACCESSION   DATAIW010000267 DATAIW010000000
VERSION     DATAIW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15934"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="VHM48_08520"
     CDS             complement(<1..1755)
                     /locus_tag="VHM48_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2755493.1"
                     /translation="MNSEGPFELDPGRLVDVLRLIADGAPNAVVAVDAEGGITFINPK
                     AATLFGYRVDELVGRPIETLLPDRLRGDHIEHRRDFLNRPRERPLGIGLELAGLRHDG
                     TEFPAEISLTPVQGPAGRIVMATVVDITARKQIEAALAESEGRFRAVLEASPNAVLAI
                     DESGLVVYANPQVAATFGFDVAELIGQPVEMLLPSRVWERHVDQRREFMEEPTARPMG
                     IGLDLSGRRANGSEFPVEISLSPVRTADGILAFATVVDITARKSLQQELLQAQKMESI
                     GRLAGGIAHDFNNMLSAIRGHVDILLDDISVAGGAIDSTEVHASLEAIRDASERAASL
                     TAQLLAFSRQQVLQPQVLDVQAVVAGLDPMLRRLIGERVQLTTVIDPATGTLLGDPGQ
                     FDQILLNLVVNARDAVSDGGEIVIGAGNATIAPDDAVEHFDVPPGEYVVVSVSDNGSG
                     IDHATREHIFEPFFTTKAVGKGTGLGLATIYGIVRQSGGHIWLYSEPGMGTTFKLYFP
                     RVDEVVDAPVRTVGGVQPAASGRLLVAEDDPAVREVIVQLLTRAGHTVLSAADGAAAL
                     ELVSRHTIDVLVSDVVMPT"
     gene            1947..>2274
                     /locus_tag="VHM48_08525"
     CDS             1947..>2274
                     /locus_tag="VHM48_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755494.1"
                     /translation="MPTWEATSHGVAWRVDLERDRLLPGRLVAGQVTVTADGETDARR
                     LVVTLVGVEAWQHEETSTNAGGHPTTRTVTSTAELPRVPVELSGPLHLARGETRTWPI
                     ELPVPPL"
BASE COUNT          346 a          785 c          818 g          325 t
ORIGIN      
        1 cgtcggcatc accacgtcgc tgacgaggac gtcgatcgtg tggcgcgaga cgagctcgag
       61 ggccgcggcg ccgtcggccg ccgagagcac cgtgtggccc gcccgggtca ggagttggac
      121 gatgacttcc cggacggccg ggtcgtcctc ggcgaccagg agccgcccgg aagccgcggg
      181 ttgaacgccg ccgaccgttc gcacgggcgc gtccacgacc tcgtcgaccc gcggaaagta
      241 gagcttgaac gtcgtgccca tcccgggctc cgagtagagc cagatgtggc cgccggattg
      301 gcggacgata ccgtagatcg tcgccagccc aaggccggtg cccttgccga ccgccttcgt
      361 ggtgaagaac ggctcgaaga tgtgctcgcg cgtcgcgtgg tcgatgccgg aaccgttgtc
      421 gctgaccgac acgacgacgt attcgccggg cggaacgtcg aaatgctcga ccgcatcgtc
      481 gggcgcgatc gtcgcgttgc cggccccgat cacgatctcg ccgccgtcgg agaccgcgtc
      541 cctggcgttg acgacgaggt tcaggaggat ctggtcgaac tggccgggat cgccgagaag
      601 cgtgccggtg gcggggtcga tgaccgtcgt cagctggacc cgctcgccga tcagccgacg
      661 gagcatcgga tcgagaccgg ccacgacggc ctgaacgtcg aggacctgcg gctggaggac
      721 ctgctggcgg ctgaacgcca ggagctgggc cgtcaggctg gcggcgcgct cggacgcatc
      781 gcggatcgcc tcgaggctcg cgtggacctc ggtcgagtcg atcgcgccgc cggcgacgga
      841 gatgtcgtcg agcaggatgt cgacgtggcc gcggatggcc gacagcatgt tgttgaagtc
      901 gtgggcgatg ccgcctgcca gccggccgat gctctccatc ttctgggcct ggaggagttc
      961 ctgctggagg gacttccggg cggtgatgtc gacgacggtc gcgaaggcga ggatcccgtc
     1021 ggccgtccgg accggagaga ggctgatctc gaccgggaac tccgagccgt tggcgcgacg
     1081 accggagaga tccaggccga tgcccatcgg ccgcgcggtc ggctcctcca tgaactcccg
     1141 gcgctggtcg acgtgccgct cccagacccg cgagggaagg agcatctcga ccggctgacc
     1201 gatcagctct gccacgtcga agccgaacgt ggccgccacc tgggggttgg cgtagacgac
     1261 gagcccggat tcgtcgatcg cgaggaccgc gttcggcgac gcctccagca ccgcccggaa
     1321 gcgtccctcc gactcggcga gtgccgcctc gatctgcttg cgggcggtga tgtcgaccac
     1381 ggtcgccatg acgatccgac cggccggacc ctggaccggc gtgaggctga tctcggccgg
     1441 gaactcggtc ccgtcgtgac gcagtccggc cagctcgagg ccgatcccca gcgggcgctc
     1501 ccggggtcgg ttcaggaagt cccggcggtg ctcgatgtgg tcgccgcgga gacggtccgg
     1561 gaggagggtc tcgatcggcc ggccgacgag ctcgtcgaca cgatatccga acagggtcgc
     1621 ggccttcggg ttgatgaaag tgatcccgcc ctcggcgtcg accgcgacga ccgcgttcgg
     1681 cgcgccatcc gcgatcagtc gcaggacgtc gaccaggcga cccggatcga gctcgaacgg
     1741 cccttcggaa ttcattgcca acccgctcgg tcaatcgcgc tggcttcgcg cgctcgatcc
     1801 actccccacc cccggccgtc ctgtaccccg agaccgggcg atactagtcc tctgttcgga
     1861 cgccgtcgat gatcgagcga ccttcgcgac gccgacgccg gcgaggaccg aaccgccggc
     1921 ccacgcgacc gccagacgag ggaacgatgc cgacctggga agccacgagc cacggcgtcg
     1981 cctggcgcgt cgatctcgag cgggaccggc tgctcccggg ccgcctggtt gcgggccagg
     2041 tcacggtcac cgcggacggc gagacggacg cccggcgact cgtcgtcacg ctggtcgggg
     2101 tcgaggcatg gcagcacgag gagacgtcga ccaacgcggg aggccatccg acgacccgga
     2161 ccgtgacgtc caccgccgag ctgccacggg tcccggtcga gctgtccggc ccgctgcacc
     2221 tggcccgggg cgagacgcgg acctggccga tcgagctgcc ggtcccgccg ctcg
//
LOCUS       DATAIW010000268         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_245390, whole genome shotgun sequence.
ACCESSION   DATAIW010000268 DATAIW010000000
VERSION     DATAIW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245390"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VHM48_08530"
     CDS             <1..1077
                     /locus_tag="VHM48_08530"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX2755495.1"
                     /translation="HMLIIKVTSPEGRSKYVAGAFPSACGKTNLAMLVPTLPGWRVET
                     VGDDICWMRPGADGRLYAINPEAGFFGVAPGTNEKTNLNAIRTLDRNCIYTNVALTDD
                     GDVWWEGLTETPPAHLIDWTGRDWTPDAGRVAAHANSRFTAPAGQDPAIAPEWQDPAG
                     VPIDAIMFGGRRATIVPLVREAFDWTHGVFMGSIMSSETTAAAIGEVGKLRFDPFAML
                     PFCGYNMADYFNHWLEVGAATRPELLPRIYYVNWFRRDADGRFLWPGFGENSRILEWI
                     FRRGDGEARATETAIGFLPEPDDLNTAGLDMAPEDLAELLRVDGPAIVAELPQFEQHY
                     ARFGDRLPASLRTELDRLRERLLA"
     gene            1074..>1894
                     /locus_tag="VHM48_08535"
     CDS             1074..>1894
                     /locus_tag="VHM48_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX2755496.1"
                     /translation="MTHPTGNAWLPAGLDRLDPAQLGGLGDMSPEQFRAAAHATVDLL
                     ADYLEHVEDFAILPPIEPGALRDQLPANPPMGPEPIGAILADVPGLVEPNATHWQHPG
                     FLAYFPTTASGPGILGEFVTAVLGQNPMLWRTSPVGTELEEVIVGWLRRALGLPDSFD
                     GLLTDTASTSSLIAIAAAREAAGLDVAALGLAGRPDLGAPVVYASEEAHSSIEKACMT
                     LGLGRIACRRIPTDGAYAMRIDALEAALAADRAAGRLPIAVVATIGTTSSTAVDPV"
BASE COUNT          270 a          685 c          668 g          271 t
ORIGIN      
        1 cacatgctga tcatcaaggt gacctcgccg gagggacgca gcaagtacgt ggccggcgcg
       61 ttcccctccg cgtgtggcaa gacgaacctg gcgatgctcg tcccgacgct gcccggctgg
      121 cgggtggaga cggtcggcga cgacatctgc tggatgcggc ccggcgcgga cggccgcctc
      181 tacgcgatca accccgaggc gggcttcttc ggcgtcgcgc cgggcacgaa cgagaagacg
      241 aacctcaacg cgatccggac gctcgatcgc aactgcatct acacgaacgt ggccctcacc
      301 gacgacggcg acgtctggtg ggagggcctc acggagactc cgcccgccca cctgatcgac
      361 tggaccggcc gcgactggac gccggatgcc ggccgcgtgg cggcccacgc gaactcccgc
      421 ttcacggcgc ccgccggcca ggacccggcg atcgccccgg agtggcagga tccagcgggc
      481 gtgccgatcg acgcgatcat gttcggcggg cggcgggcca ccattgtgcc gcttgtccgc
      541 gaagcgttcg actggacgca cggcgtcttc atgggctcga tcatgagctc cgagacgacc
      601 gcggcggcga tcggcgaggt tgggaagctg cgcttcgacc cgttcgccat gctgccgttc
      661 tgcggctaca acatggccga ctacttcaac cactggctgg aggtcggcgc cgccacgcgc
      721 ccggagctac tgccccggat ctactacgtc aactggttcc ggcgcgacgc cgatggacgg
      781 ttcctgtggc cgggcttcgg cgagaacagt cgcatcctgg agtggatctt ccggcgcggc
      841 gacggcgagg cgagggcgac cgagaccgcg atcgggttcc tgccggagcc cgacgacctc
      901 aataccgccg ggctcgacat ggcgcctgaa gacctcgcgg agttgctgcg ggtggacggc
      961 cccgccatcg ttgccgagct gccccagttc gagcagcact acgcgcgctt cggcgaccgg
     1021 ctgccggcct ccctgcgaac cgagctggac cggctccggg agcgcctcct cgcgtgaccc
     1081 atccaaccgg caacgcctgg ctcccggcgg gcctcgaccg cctggacccg gcccagctcg
     1141 ggggcctcgg cgacatgagc ccggagcagt ttcgagcggc cgcccacgcg acggtggacc
     1201 tgctcgcgga ctacctcgag cacgtcgagg acttcgcgat cctgccgccg atcgagccgg
     1261 gcgcgctgcg cgaccagctg ccagcgaacc cgcccatggg cccggagccg atcggggcga
     1321 tcctcgccga cgtgccgggt ctcgtggagc cgaacgccac gcactggcag caccccggct
     1381 tcctggcgta tttcccgacg acggcgtcgg ggccggggat cctcggcgag ttcgtcacgg
     1441 cggtgctggg ccagaacccg atgctctggc ggacctcgcc ggtcgggaca gagctggagg
     1501 aggtgatcgt cggatggctc cgtcgggcgc tcggcctgcc cgattcgttc gatgggctcc
     1561 ttaccgacac ggcctcaacg tcgtcgctca tcgcgatcgc agcggcgcgc gaggcggctg
     1621 ggctcgacgt ggccgccctc ggccttgccg gacggccgga tctcggcgcg cccgtcgtct
     1681 acgcctcgga ggaggcccac agcagcatcg agaaggcgtg catgacactg gggctgggcc
     1741 ggatcgcctg ccgccggatc ccgaccgacg gcgcgtacgc gatgcggatc gatgcgctcg
     1801 aggcggcgct tgccgcggac cgggccgccg ggcggctgcc catcgctgtc gtggcgacga
     1861 tcggtaccac ctcgtcgacc gccgtggacc ccgt
//
LOCUS       DATAIW010000269         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_76352, whole genome shotgun sequence.
ACCESSION   DATAIW010000269 DATAIW010000000
VERSION     DATAIW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76352"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHM48_08540"
     CDS             complement(<1..224)
                     /locus_tag="VHM48_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755497.1"
                     /translation="MTAADGPAVLEIYGQGIAGGIATFETVVPDWAHWHTSHRRDCRF
                     VARLDGRVVGWTALGRYSTRAVYEGVAWESV"
     gene            complement(266..1399)
                     /locus_tag="VHM48_08545"
     CDS             complement(266..1399)
                     /locus_tag="VHM48_08545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2755498.1"
                     /translation="MSHRDASPAQAGDLVFPALPDGLDTPRVVVDLARVTANIGRLQG
                     EMDRRGIALRPHAKTHKSAAIARRQIEAGATGITVGTLGEAEVFVGAGITDVFLAYPV
                     WAVGPKALRLRAVHEAASRFRVGADSVGGVDHLAAAVAGTERPLTVLVEIDPGNHRTG
                     LASPEAAVEVARAARGAGLIVEGVFSHGGHGYRIGSMASAGADEARSLGVAAEALRRD
                     GFAIGTISAGSTPTMLSAATGAVNEMRAGTYVYGDRQQWALGAIGADGCAVAVVATVV
                     SVHAERLVLDAGAKALTKDRADWLTGFGAIVGYPDLVIERVNDYHGVVAAPPGVDRPR
                     LGQVVAVIPNHVCPVIDLVDRVVAVAADGSVEEWPVDARGRSG"
     gene            complement(1396..2439)
                     /locus_tag="VHM48_08550"
     CDS             complement(1396..2439)
                     /locus_tag="VHM48_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2755499.1"
                     /translation="MKLAHVRDVHAPAGAPWRLAAGLASDDDGPAGPLHWIDLEVARR
                     RAVTGDPALAHDRALFRQPVTTLDDHLARGLRVAVLADLVEGFVPRDDGGDDPAVLDA
                     AELRFGPPVLRPPSLRDFYAFEGHVRTMWERRGGEVPETWYRLPIFYFSNVSEIRGPD
                     EPVWSPAASSELDYELEVAAVVDTPVVDLPAERGEEAIGGYTIFNDWSARDLQREETV
                     VRLGPAKGKDFAASFGPWLVTPDELDVAAARAAGASGPDLAMTAEVNGVETSRGRWSD
                     AQFSFGEMVARAAADARLRPGDLIGSGTVGSGCLLEVRDATLGRYLEPGDQVVLRIDR
                     LGALRTPIVARSR"
     gene            2556..>4873
                     /locus_tag="VHM48_08555"
     CDS             2556..>4873
                     /locus_tag="VHM48_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2755500.1"
                     /translation="MSDLPARTPTAPGGDAPGSGAPAFSVARAAAPPQHPSAVPGDIA
                     VLVVIVRLIALVATLIGAAEVVAGLAATEPRAVILGGAAVIYGVWVATRARVFAGSGR
                     ESTITSIAVMTLGLIAIAAALQPSMATAMAIASLLPAVIVTPIVTSRLVLRLLVLSGI
                     VAAWSVLVAELAPRSRLPADTQSALAFIVLVLAYAFLIISIWEVSRRLKGTASDLRSV
                     VTMSGDLAETLDPRLVGDRIAVHIARAVGADDCALSYWDRATDQVVTLGYDPPERRAA
                     LKETYHLDEFPATRRVLVEQRPVLIDADDPAADPAEAHYLASIGQRTMAMIPLVAAGR
                     TVGLVEVTSSRKAAFDSRSVELAAMLAGEAAMALENARLYEEIRHQALHDGLTGLANR
                     VLFRDRVEHAVARSRRSGTRIAVLFVDLDDFKALNDTHGHARGDEVLAISARRVTEAL
                     RPSDTAARQGGDEFAILIEDVVDDADALAVATRLADALRQPMSIGHAEVRIAASIGVA
                     LGGAGQESADDLLRNADVAMYAAKGSSRGGAEIFRPSLRDGATERAEMAARLRGIEER
                     DELRLDYQPIVELATNRIEGVEALVRWQPSDGPVLMPTEWIELAEESGDIVPIGRWIL
                     REACRQARDWQIRLARPDLRMSVNLSARQFREHDVVATVRAILDETHLPPATLTLEIT
                     ESGLMRRTQATITRLEALRALGVHLAIDDFGTGYSSLSYLERFPIDILKIDRTFVSSV
                     GDVVSPIARAIVDLGRTLGLQVIAEGIEHIEQA"
BASE COUNT          698 a         1802 c         1697 g          676 t
ORIGIN      
        1 acgctttccc aggcgacgcc ttcgtagacc gcacgggtcg aatagcgccc cagcgccgtc
       61 cagccgacga cgcggccgtc gagccgggcg acgaaccggc aatcgcggcg atgcgaggtg
      121 tgccagtggg cccagtcggg cacgaccgtc tcgaacgtcg cgatgcctcc tgcgatgccc
      181 tggccataga tctcgaggac ggcggggccg tcagcggcgg tcatcggctc gatcgacagg
      241 cgttcgcggt ccctcccctg caccgtcagc cgctccgccc gcgggcgtcg accggccact
      301 cctcgacgga tccgtcggcc gcgaccgcga ccacccggtc gaccaggtcg atgaccgggc
      361 agacgtgatt ggggatgacc gcgacgacct ggcccaggcg cggtcgatcg acgcccggcg
      421 gcgcggcgac cacgccgtga tagtcgttga cgcgctcgat gacgaggtcc gggtagccga
      481 cgatcgcgcc gaacccggtc agccagtcgg cccggtcctt cgtcagcgcc ttcgcccccg
      541 cgtccaggac gagccgctcg gcgtggactg acacgacggt cgcgacgacc gcgaccgcgc
      601 agccgtcggc gccgatcgcg ccgagggccc attgctgacg gtcaccgtag acgtaggtcc
      661 cggcgcgcat ctcgttcacc gcaccggttg ccgcgctgag catcgtcggg gttgacccgg
      721 cgctgatcgt gccaatcgcg aagccgtctc gccggagggc ctcggccgcc accccgagcg
      781 agcgagcctc atcggcgccg gcgctggcca tgctcccgat ccgatagccg tgcccgccgt
      841 ggctgaagac gccctcgacg atgaggccgg ccccgcgtgc cgcccgggcg acctcgacgg
      901 cggcctcggg cgaggcgagc cccgtccggt gattgcccgg atcgatctcg acgaggaccg
      961 tcagcggccg ctccgtgccg gcgacggcgg ccgcgagatg gtccacgccg ccgaccgaat
     1021 cggcgcccac ccggaaccgc gacgccgcct cgtggacggc acggagtcgg agcgccttgg
     1081 ggcccacggc ccagaccggg taggccagga agacgtcggt gatgcccgca ccaacgaaca
     1141 cctcggcctc gccgagcgtg ccgacggtga tcccggtcgc gcccgcctcg atctggcgcc
     1201 gcgcgatcgc cgcgctcttg tgggtcttgg cgtgcggccg gagggcgatg ccgcggcgat
     1261 ccatctcgcc ctggagccgc ccgatattcg cggtcacccg ggcgaggtcg acgacgaccc
     1321 gcggcgtgtc gaggccgtcg ggcagggccg ggaagaccag gtcgccggcc tgcgccggac
     1381 tcgcatcgcg atggctcatc gcgagcgggc cacgatcggc gtccggaggg cgcccagccg
     1441 gtcgatccgc agcacgacct ggtcacctgg ctccaggtat cggccgagcg tcgcgtcccg
     1501 gacctcgagc aggcagccgc tgccgacggt ccccgaaccg atcaggtcac cgggtcggag
     1561 ccgggcgtcg gccgccgctc gggccaccat ctcgccgaac gagaactggg cgtccgacca
     1621 gcggccgcgt gacgtctcga cgccgttgac ctcggcggtc atcgccaggt ccggaccgct
     1681 cgctccggcc gcgcgggccg ccgcgacatc cagctcgtcc ggggtcacca gccacggccc
     1741 gaaggacgcg gcgaagtcct tgcccttggc cggcccgagc cggacgacgg tctcctcgcg
     1801 ctggaggtcg cgggccgacc agtcgttgaa gatcgtgtag ccgccgatcg cctcctcgcc
     1861 tcgctcggcc gggaggtcca cgaccggcgt gtcgacgacg gcggccacct ccagctcgta
     1921 gtcgagctcc gatgacgccg ccggcgacca gaccggctcg tccggtccgc ggatctcgga
     1981 cacgttgctg aaatagaaga tgggcaggcg gtaccacgtc tccggaacct cgccgccgcg
     2041 ccgctcccac atcgtccgga cgtgaccctc gaacgcatag aagtcgcgca ggctgggggg
     2101 tcggaggacc ggcgggccga accggagctc ggccgcgtcc aggaccgccg ggtcgtcgcc
     2161 gccgtcgtcg cggggaacga agccttcgac gagatcggcg aggacggcga cccggaggcc
     2221 gcgagccaga tggtcgtcga gggtcgtgac cggctggcga aagagcgccc gatcgtgggc
     2281 cagggcggga tcgccagtca cggcccgccg tcgggcgacc tcgagatcga tccagtggag
     2341 cggtccggcg gggccgtcat cgtccgacgc gagtccggcc gccagacgcc acggtgcccc
     2401 ggccggcgcg tgcacgtcgc ggacgtgggc gagcttcatc gacggatggg cgacgtcggg
     2461 agcatggccg gagtgtaggc caccacccga tcggcgaact ccgttcgtca ttcgggggat
     2521 gacgaagccc ggacctcgat gccagcctga cggagatgag cgaccttccg gcccgcacgc
     2581 cgacggcgcc gggtggcgac gcgcccggat ccggcgcgcc cgcgttcagc gtggcgcggg
     2641 cggccgcgcc cccgcagcat ccatcggcgg tcccgggcga catcgccgtc ctcgtcgtca
     2701 tcgtccgctt gatcgcgctg gtcgccacct tgatcggagc ggccgaagtg gttgcgggcc
     2761 tggccgccac ggagccgcga gccgtcatcc tgggtggagc tgccgtgatc tacggcgtgt
     2821 gggtcgccac ccgcgcccgg gtctttgccg gatcgggccg ggagtcgacg atcacctcga
     2881 tcgcggtcat gaccctcgga ctcatcgcga tcgccgcggc cctccagccg tccatggcaa
     2941 cggcgatggc gatcgcctca ctgcttccgg cggtgatcgt caccccgatc gtcacgagcc
     3001 ggctcgttct ccgactgctc gttctcagcg ggatcgtcgc cgcgtggagc gtcctcgtcg
     3061 ccgagctcgc gccgcggagc cgacttccgg cggacacgca atcagccctc gccttcatcg
     3121 tcctcgtcct ggcctatgcc ttcctgatca tctcgatctg ggaggtcagc cgccgcctca
     3181 agggcaccgc gtcggacctg aggagcgtcg tgacgatgtc cggcgacctc gccgagaccc
     3241 tcgatcctcg cctcgtcggg gaccggatcg ccgttcacat cgcccgggcg gtcggggccg
     3301 acgactgcgc cctgagctac tgggaccggg ccaccgacca ggtcgtgacc ctgggctacg
     3361 acccgccgga acgacgcgcg gccctgaagg agacctatca cctcgatgag ttccccgcga
     3421 cccggcgggt cctggtcgag cagcgacccg tcctcatcga cgccgacgat cccgccgccg
     3481 acccggcgga ggcgcactac ctcgcctcga tcggccaacg gacgatggcg atgatcccgc
     3541 tcgtggccgc cgggcggacc gtcggcctgg tcgaggtcac gtcgagccgc aaggccgcat
     3601 tcgacagccg ctcggtcgag ctcgcggcga tgctggccgg ggaagccgcg atggccctcg
     3661 agaatgcccg gctgtacgag gagatccgcc accaggcgtt gcacgacggc ctgaccggcc
     3721 tcgccaatcg cgtcctcttc cgtgaccgcg tcgagcatgc cgtcgcgcgg agtcgacgga
     3781 gcggcaccag aatcgccgtc ctcttcgtcg atctcgacga cttcaaggcg ctcaacgaca
     3841 cccacggcca cgcgcgcggc gacgaggtgc tggcgatctc cgcgcggcgg gtgaccgaag
     3901 ccctgcggcc atccgacacg gccgcccggc agggcggcga cgagttcgcg atcctgatcg
     3961 aggacgtggt cgacgacgcc gatgcattgg ccgtcgcgac ccgtcttgcc gacgccctcc
     4021 gccagccgat gtcgatcggc cacgccgagg tgcggatcgc cgccagcatc ggggtggccc
     4081 tcggtggtgc cggtcaggaa tcggccgacg atctgctccg caacgccgac gtggcaatgt
     4141 atgccgcaaa gggctcgtca cgaggcggcg ctgagatctt ccgtccatcc ctccgcgacg
     4201 gggcaaccga gcgagccgag atggccgccc gactgcgcgg catcgaggag cgcgacgagc
     4261 tccgcctgga ctatcagccg atcgtcgagc tcgcgaccaa ccggatcgag ggtgtcgagg
     4321 cgctcgttcg atggcagcca tccgacgggc cggtcctgat gccgaccgaa tggatcgagc
     4381 ttgccgagga aagcggcgac atcgtcccga tcggccgttg gatcctgcgc gaggcatgtc
     4441 gtcaggcccg cgactggcag atccgcctcg cccggccgga tctccggatg agcgtcaacc
     4501 tgtccgcccg gcagttccgc gagcacgacg tcgtcgccac ggtccgggcg atcctcgacg
     4561 agacccacct gccgccggca acgctgacgc tcgagatcac cgagagcggc ctgatgcggc
     4621 ggacccaggc cacgatcacc cggctcgagg cgctccgggc cctcggcgtc cacctcgcga
     4681 tcgacgactt cgggaccggc tattcgtcgc tcagctatct cgagcggttc ccgatcgaca
     4741 tcctcaagat cgatcggacg ttcgtctcgt cggtcggcga tgtcgtgtca ccgatcgccc
     4801 gtgcgatcgt cgatctcggc cggacgctcg ggctgcaggt catcgccgag gggatcgagc
     4861 acatcgagca ggc
//
LOCUS       DATAIW010000270         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_215654, whole genome shotgun sequence.
ACCESSION   DATAIW010000270 DATAIW010000000
VERSION     DATAIW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215654"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VHM48_08560"
     CDS             <1..673
                     /locus_tag="VHM48_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX2755501.1"
                     /translation="WDAAVERWRTLPSDDGAVYDKSITIDASALEPMVTYGTNPGMGI
                     PITSRVPAPADLADGSARRALERALEYMDLQPGQAILGQKVDVVFVGSCTNGRISDLR
                     LAAEVLRGRTVADGVRMMIVPGSDDVKREAEREGLHEIFRAAGAEWREAGCSMCIAMN
                     GDQLSPGQYAISTSNRNFEGRQGKGGRTFLASPLTAAASAIRGEVTDPRTLPGIETMA
                     AVGGR"
     gene            676..1299
                     /gene="leuD"
                     /locus_tag="VHM48_08565"
     CDS             676..1299
                     /gene="leuD"
                     /locus_tag="VHM48_08565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2755502.1"
                     /translation="MAERFRTFTSAVIPLAAENVDTDQIVPARYLKVTTKDGLREALF
                     RDWRYDEDGGLKEPPFVMDRVAMLDRQILLVGDNFGTGSSREHAPWALTAWGIRAILS
                     TSFADIFRNNALKNGLLPIVVEAEKHRQLFELVATNPRAELTVDLEAQVIHLPGDEDL
                     PFEVDPFARLMLLNGTDEMGYLLGKLPAIEAWEAGHPARIDTRAGAA"
     gene            complement(1365..1496)
                     /locus_tag="VHM48_08570"
     CDS             complement(1365..1496)
                     /locus_tag="VHM48_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755503.1"
                     /translation="MTRTTSRPVNYRITRRMAAVHRADATAPHLGCVLCLVGRAPGR"
BASE COUNT          281 a          708 c          710 g          264 t
ORIGIN      
        1 atgggacgcg gccgtcgagc gctggcggac gttgccgagc gacgatggcg ccgtgtacga
       61 caagtcgatc acgatcgacg cctcggccct cgagccgatg gtcacctacg gcaccaaccc
      121 cgggatgggg atcccgatca cctcgcgcgt cccggccccg gccgacctcg ccgacggatc
      181 ggccaggcgc gcgctcgagc gggccctcga gtacatggac ctccagcccg gccaggcgat
      241 cctcggccag aaggtcgacg tggtcttcgt cggctcgtgc acgaacgggc ggatcagcga
      301 tctccgtctc gcggccgagg tccttcgcgg ccggacggtg gccgacgggg tccggatgat
      361 gatcgtgccc ggctccgacg acgtgaagcg tgaggccgag cgtgagggcc tccacgagat
      421 cttccgggcc gccggcgccg agtggcgcga ggcgggatgc tcgatgtgca tcgcgatgaa
      481 cggcgatcag ctgtcgcccg gccagtacgc gatcagcacc tcgaaccgaa acttcgaggg
      541 tcgccagggc aagggcggcc ggacgttcct cgcctcgccg ctgaccgcgg cggcgtcggc
      601 gatccgtggc gaggtcaccg acccgcggac cctgcccggc atcgagacga tggccgcggt
      661 cggaggccgc tgacgatggc cgagcgcttc cggaccttca ccagcgccgt cattcccctg
      721 gcggccgaga acgtcgacac cgaccagatc gttcccgctc gctacctcaa ggtcaccacc
      781 aaggacggtc tccgcgaggc cctcttccgg gactggcgct atgacgagga cggcgggctc
      841 aaggagccgc cgttcgtgat ggatcgggtc gcgatgctcg atcggcagat cctcctcgtc
      901 ggcgacaact tcgggaccgg ctcgtcgcga gagcatgcgc cgtgggccct gaccgcgtgg
      961 ggcatccggg cgatcctgtc gacttcgttc gccgacatct tccgcaacaa cgccctgaag
     1021 aacgggctgc tgccgatcgt cgtcgaggcc gagaagcatc gccagctgtt cgagctggtc
     1081 gcgaccaacc cccgggcgga gctgaccgtc gacctcgaag cccaggtcat ccacctgccc
     1141 ggcgacgagg acctcccctt cgaggtcgac ccgttcgccc ggctgatgct cctgaacggg
     1201 accgacgaga tgggctatct cctcgggaag ctccccgcga tcgaggcctg ggaggcgggc
     1261 catcccgccc ggatcgacac gcgcgccggg gccgcctgag cgccggaccg gacctcgagc
     1321 cgcccgcccg gcggagggcg ggcgcggtcg tcgggcggag gagatcagcg gccgggggcg
     1381 cgcccgacca ggcagaggac gcagccgagg tgtggggccg tcgcgtcggc gcggtggacc
     1441 gcggccatcc ggcgggtgat ccggtagttg acggggcggg aggtcgtgcg ggtcatcggg
     1501 agcggtcctt tcgatcgatg gtggctcctc tcggaacgag tcccgatcgt cgccgctcat
     1561 cgccgcggac ggcatcgccg cacgaccttg atcggcatgg acaggtgacg atgccgggcg
     1621 tcgcccggcg gggaacccgc ggtcgatcgc ggtcgtctgc cgggccatga cgaccacgcc
     1681 gaccacccgc cgaccgatcc tcgccggcgc catcgccatc gccatcgcca tcgggctcgc
     1741 cgcgtgttcg ggcgcggcgg cgccggcgac aacgccgtcg ggcgacgcca cggcatcccc
     1801 ggtgccagcc tcggcgccgc ctaacggagc cgatccgggc gcgggcgcgg gcggcggcgg
     1861 ctcagccggg aatccgggca ccggaatcgg gatcgttccc gtggaccccg cacccgtcga
     1921 tccgggagcc gggcaaccca tcctcgtccg gccggtcccg ggg
//
LOCUS       DATAIW010000271         8526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_165996, whole genome shotgun sequence.
ACCESSION   DATAIW010000271 DATAIW010000000
VERSION     DATAIW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165996"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHM48_08575"
     CDS             <1..255
                     /locus_tag="VHM48_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755504.1"
                     /translation="RAGWTAVVSHRSGETEDTTIADFVVAMGTGQIKTGAPSRSERVA
                     KYNRLLRIEGELGGGAVYLGHAALGRKTADDAAHEVGTAR"
     gene            252..839
                     /locus_tag="VHM48_08580"
     CDS             252..839
                     /locus_tag="VHM48_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755505.1"
                     /translation="MTRFVDRGAIVAATVGAGMAVTMAISFLLVIPIEPIYWLLAVPA
                     GLLIGYYANQRSERRAGPWSRIVANAAFAGLVTGLTMAALLLVVKALFFFGDNGYPDF
                     NRIDAQTRLAVPPACDSGAGCVYARYLLVGDGPEFAAAGITDASTFTGFYWAQQASST
                     GTVLVMTLLGGFGGGVLYGTFRPKPARTKSSAVPG"
     gene            complement(921..1598)
                     /gene="rpsT"
                     /locus_tag="VHM48_08585"
     CDS             complement(921..1598)
                     /gene="rpsT"
                     /locus_tag="VHM48_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX2755506.1"
                     /translation="MAKTPRTWTGARTPSGLKRVRQAERRRDILQPRRSAAKTFVANA
                     LSVATHDGDPEVAAKALADAHAALDRAAKTGAIHANAAARRKSRLTLKFNAATGGVAV
                     QTSGRVVKTTSKAQAAKAAKARIAAGKANKAKGAQTAAGKARAAVSKTARAEAAAAAS
                     AAASSTAEAPKTASKASAAKATTKKAPTKKAPTKKAPAKAKATAAKAPAKATAKKAPT
                     KKAAAKS"
     gene            1921..2838
                     /locus_tag="VHM48_08590"
     CDS             1921..2838
                     /locus_tag="VHM48_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2755507.1"
                     /translation="MLAILGGFGAALCWATSSMASARSARLIGSWATLGWVMLIGTVI
                     AVPATILGGAGADLTPTALQLLAISGVGNIVGLLLAYTAFQGGKVAVIAPILSTEGAM
                     GAVISIVFGEQVGLTAAVVLTAIAVGIVLASAGGTDEALAAEPGGLALDHGALPAADP
                     AEGPGHPDRKATLVALGAVVFFGINLYASARIGATLPIVWAVLPARLGGFVFVALPLL
                     ATRRLRLTREAVPFVIVVALTELVGTAVYAFGARDGIAVAAVTSSQFGAIAAVVSVVA
                     FGERLRRIQVAGVVVIAAGVALLAALQAS"
     gene            3169..4980
                     /gene="lepA"
                     /locus_tag="VHM48_08595"
     CDS             3169..4980
                     /gene="lepA"
                     /locus_tag="VHM48_08595"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX2755508.1"
                     /translation="MTIPQDRLRNFSIIAHIDHGKSTLADRLLELTHTIEGRQMMSQV
                     LDSMDLEREKGITIKARAVRLEYTARDGLTYGLNLIDTPGHVDFAYEVSHSLQACEGA
                     ILVVDATQGIEAQTLANVHLALRENLTLIPVLNKIDLPSADPETIMDELENVLAIPRE
                     EVILASAKDGTGVPEILEAIVARIPAPKGDPTKPVQGLIFDSHYDAYKGVIVYVRLAA
                     GTLRQHDQIRLMASGAIAEPLELGVFRPQLVPVNQLVAGEVGYVATGLKSVRDAQVGD
                     TVTTVANPAAKPLPGYQPAKSLVFAGIYPVSGEDYPLLREALERLHLNDASFTYEPES
                     SVALGFGFRCGFLGLLHMEIVQERLEREFDLDLIASAPSVEYHVKLLYGRGEVSIDNP
                     SLLPNPSDIEVLEEPWVNLSVVTPSSYIGPLMELSTNRRGLFQNMEYLDPTRVVLHFE
                     MPLAELIIDYFDQLKSRSQGYASMDYEELGYRAANLAKVDILVNGEPVDALSLIVHRD
                     KAQSAGRVLTERLKQLIPRQMFEIPIQAAIGSNVIARETVRAMRKNVLAKCYGGDITR
                     KRKLLEKQKEGKQRMKRVGSVEIPQEAFLAMLRMDEG"
     gene            4987..5754
                     /locus_tag="VHM48_08600"
     CDS             4987..5754
                     /locus_tag="VHM48_08600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2755509.1"
                     /translation="METWLPTIRTLIAIGLALLLVMLRLEAVRFGAAEYDDPVDGRAP
                     SVRRRVAWYAVGILLVVGILLVHPRAQEDFALSAGDRSQAFVYGFLYAALGTAIALGI
                     AFWRYRRLRFPDVWSYPGALLNAVVTALIDEVAFRGAVLGLLLLTGLEPTAANAIQAI
                     LYALATRLGAPGRNRWMLVMAILVGLAGGWVTAVTGGIAAAFIGHAVTRFAVFLSTGH
                     AGQFLPRGREVEEIEATRRPPKGWRVIGSRDPTSRDR"
     gene            5966..7240
                     /locus_tag="VHM48_08605"
     CDS             5966..7240
                     /locus_tag="VHM48_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX2755510.1"
                     /translation="MGAARDATPPGRDGLLTPRPPAGLYLHIPFCVSLCPYCDFVVVV
                     GAAARGPANRIGALAAALRTELELRADTLDAAYGKPGSASRPALDTVYIGGGTPTLVP
                     ADIVATLLETVDRRYGIAPDAEVTIEANPGPDERGDAAALARAGINRISFGAQSLDAG
                     ELRRLGRRHRPSDVADAVAGARAGGIGSVNLDLLYDVPDGSLATWIETLEQALALEPD
                     HLSLYALTLDDPDAEGLTGADGDHLPTTRGARRWRDAAIPAQDEDRAAGQYHHAVHRL
                     AADGWRGYEISNWARPGHESRHNLAYWERRPYEAVGPGAHAFDGATRRWNAANLARYL
                     MALAPAGDEPARLPPGGAETLDAATALAESIVLGLRTDRGLPRAAATKPPLADVFGWA
                     LAAELLTIDDGDRIVLTTRGRLLSNELFARLV"
     gene            7392..8453
                     /gene="hrcA"
                     /locus_tag="VHM48_08610"
     CDS             7392..8453
                     /gene="hrcA"
                     /locus_tag="VHM48_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX2755511.1"
                     /translation="MARRTRTSGSLDPRAESILRAVIDEYVSTAVPVGSHSLVERYGL
                     GVSSATVRNILAALEFNGLLTHPHTSAGRIPTDAGYRHFVESISSASQLPQVEQLMIR
                     HQFGQVQHASEQWFRLAATTLATANRTAGLATQAKPRAAHVRRLDLVSISDRLASLII
                     VFREGALRQVLVTLAEPVEQEDLTRAAVILDGLMAGRTAVDAERALARIDGDGPIGTL
                     VRRVGERVLAAVRDYDSTGIEEVFSDGLLHVMEAPEFERSDKLRRVFAALENRAYLGE
                     LLDRVAGTGTVQVFIGAENAPEEMRDVSLVLAPYGEPGRAVGVVGVLGPTRMAYPQAI
                     GTVRFVSTLMNELVQHIYA"
BASE COUNT         1177 a         3132 c         2904 g         1313 t
ORIGIN      
        1 cgcgccgggt ggacggccgt cgtgtcgcat cgctcgggcg agaccgagga cacgacgatc
       61 gcggacttcg tcgtcgcgat ggggaccggc cagatcaaga ccggcgcacc gtcacggagc
      121 gagcgggtcg ccaagtacaa ccggctcctc cggatcgagg gcgagctcgg cgggggtgcg
      181 gtgtacctcg ggcacgccgc tctcgggcgg aagacggcgg acgacgcggc ccacgaggtc
      241 ggtacggccc ggtgacccgg ttcgtcgacc gcggcgcgat cgtcgccgcc accgtcggag
      301 ccgggatggc ggtcacgatg gcgatcagct tcctgctcgt catcccgatc gaaccgatct
      361 attggctgct ggccgtgccg gccggcctcc tgatcggcta ctacgcgaac cagcgcagcg
      421 agcggcgggc cggtccgtgg tcgcggatcg tcgcgaatgc ggcgttcgct gggctggtca
      481 ctggcctgac gatggccgcg ctgcttctcg tcgtgaaggc gctcttcttc ttcggcgaca
      541 acggctaccc ggacttcaac cggatcgacg cccagacgcg cctggctgtc ccacccgcct
      601 gcgacagcgg agcgggctgc gtctatgcgc gctatctcct ggtcggtgac ggtccggagt
      661 tcgcggccgc aggcatcacc gatgcctcga ccttcaccgg cttctactgg gcccagcagg
      721 cctcgtcgac cggcacggtc ctggtgatga cgctcctcgg cgggttcggc ggcggcgtgc
      781 tctacggcac gttccgcccg aaaccggcca ggacgaaaag ctccgccgtt ccgggctgac
      841 ggcccgcgga ggcggggccg gacatgacga cgccccggac gaggtccggg gcgggtcgtg
      901 tcgcgcgaag aacggacggg tcagctcttg gcggcggcct tcttcgtcgg tgccttcttg
      961 gcggtggcct tggccggtgc cttggccgcg gttgccttgg ccttcgccgg cgccttcttt
     1021 gtcggcgcct tctttgtcgg cgccttcttt gtcgtcgcct tggccgcgct ggccttcgac
     1081 gccgtcttcg gcgcctcggc cgtgctcgat gcggcggccg atgcggctgc ggcagcctcg
     1141 gcgcgggccg tcttcgagac cgcggctcgg gccttgccgg cagcggtctg agcgcccttg
     1201 gccttgttgg ccttgccggc tgcgatccgg gccttggccg ccttcgctgc ctgggccttc
     1261 gaggtcgtct tcacgacgcg acccgaggtc tggacggcga cgccaccggt tgcggcgttg
     1321 aacttcaggg tcaggcgcga cttgcgacgc gcggccgcgt tcgcgtggat cgcgccggtc
     1381 ttggccgcgc gatcgagcgc cgcgtgggcg tcggcaaggg ccttcgcggc cacctccgga
     1441 tcgccatcat gcgtcgcgac gctcaaggcg ttggcgacga aggtcttggc ggcactgcgc
     1501 ctcggctgaa ggatgtcgcg ccggcgctcg gcctgacgga cgcgcttcag tcccgacggc
     1561 gttcgtgcgc ccgtccaggt gcggggcgtc ttggccaagg ggtcgaacct ctcagaaggg
     1621 ggatgtctgg ggcgtgaaca cccggcacgg gtcggtgccg agcaggacgc gcatcgtatc
     1681 agaggctcgg ccgcgtgacc aaatccgacc cgggcgagcc cggtcgggct tgtccgggaa
     1741 tcgatgggct tccggcgatt cccgcccgcg ggcgtgtcgg acgagcactg cgcccaggta
     1801 accgaatctg gcccgtgcag catctgatcg cgcacagccc gatctggtca gatacgcccg
     1861 tcccacggga attccggagc ggctcgtcgc gacccgctcg atgcggcaga cttcgccatc
     1921 gtgctggcga ttcttggcgg cttcggtgcc gcgctgtgct gggccacctc atcgatggcg
     1981 tccgcccgct cggctcgcct gatcgggtcg tgggcgacgc tcggctgggt gatgctgatc
     2041 gggaccgtca ttgctgtacc ggcgacgatc ctcggtggtg ccggcgcgga cctcaccccg
     2101 accgccctgc agctgctcgc gatctccggc gtcggcaaca tcgtcggcct gctcctcgcc
     2161 tataccgcct tccagggcgg caaggtcgct gtcatcgccc cgattctctc gaccgagggg
     2221 gcgatgggcg ccgtcatctc gatcgtgttc ggggagcagg tcggcctgac ggcggcggtc
     2281 gtgctgacgg cgatcgcggt cgggatcgtc ctggcgtcgg ccggcggcac cgacgaggcg
     2341 ctcgcggccg agccgggtgg gcttgcgctc gaccatggcg cgctcccggc ggccgacccg
     2401 gcggagggac ccggccatcc ggatcgcaag gcgacgctcg tcgccctggg cgcggtcgtc
     2461 ttcttcggga tcaacctcta tgcgagcgcc cggatcggcg cgacgctgcc gatcgtctgg
     2521 gcggtccttc ccgcccggct cggcgggttc gtcttcgtcg ccctgcccct gctcgcgacc
     2581 cggcgattgc ggctgacccg cgaggcggtc ccgttcgtca tcgtcgtggc cctgaccgag
     2641 ctcgtcggga ctgcggtcta tgcgttcggc gcgcgcgacg ggatcgcggt cgccgcggtc
     2701 acgagctccc aattcggcgc gatcgccgcg gtcgtctcgg tcgtcgcgtt cggcgagcgg
     2761 ctgcgccgga tccaggtggc gggcgtcgtg gtcatcgcgg cgggcgtcgc gctcctcgcg
     2821 gccctccagg cgagctgagg gtcgccgtcg cgtcaggccg tggccggggc tcatccggga
     2881 ttcaagcgtt cgcgaccgag atcacgcgcg aacgcccgcc ggccaccctc cgtgtcgcca
     2941 tccagcgctc ctcggtggcg tcgccacgcc tggttcgtgg cacaccgtgt gggtctcccg
     3001 cggtggtcgc cggggagagt ccggcgcggc gggagcatgg caccggccga ttcgcgaggc
     3061 aagtggaaag cgccggggaa ttgcgcggtg cgccgctccc cgatgatcga ccgcgatgtc
     3121 ccgcggccct ggccgccgcg accgtccgac cggctacact cccgcgtcgt gaccattccc
     3181 caggaccggc tgcgcaactt ctcgatcatc gcccacatcg atcacggcaa gtcgaccctc
     3241 gccgaccggc tcctcgagct gactcacacg atcgagggtc gccagatgat gagccaggtc
     3301 ctcgactcga tggacctcga gcgcgagaag gggatcacga tcaaggcccg cgccgtccgc
     3361 ctcgaataca cggccaggga cggcctgacc tacggcctca acctcatcga cacgcccggc
     3421 cacgtcgact tcgcctacga ggtgagccac agcctgcagg cctgtgaggg cgcgatcctc
     3481 gtcgtcgacg cgacccaggg gatcgaagcc cagaccctcg ccaacgtcca tctcgccctg
     3541 cgcgagaacc tgaccctgat cccggtcctc aacaagatcg atctgccctc ggccgacccc
     3601 gagacgatca tggacgaact cgagaatgtc ctcgcgatcc cacgcgagga ggtgatcctc
     3661 gcgagcgcca aggacgggac cggcgtcccg gagatcctcg aggcgatcgt cgcccgcatc
     3721 ccggccccga aaggcgatcc gaccaagccg gtccagggcc tgatcttcga cagccactac
     3781 gatgcctaca agggcgtgat cgtgtacgtc cggcttgccg cgggcaccct ccgccagcac
     3841 gatcagatcc ggctgatggc gtcgggcgcc atcgccgaac cgctcgagct cggcgtgttc
     3901 cggccccagc tcgtgcccgt caaccagctc gtcgccggcg aagtcggcta cgtcgcgacg
     3961 gggctcaaga gcgttcgcga cgcccaggtc ggcgacacgg tcacgaccgt cgcgaacccg
     4021 gcggcgaagc cgctgcccgg ctaccagcca gccaagagcc tcgtcttcgc cgggatctac
     4081 ccggtcagcg gcgaggacta cccgctcctc cgggaggcgc tcgagcggct ccacctcaac
     4141 gacgcgagct tcacctacga gccggagagc tccgtcgcgc tcgggttcgg gttccgctgc
     4201 gggttcctcg gcctgctcca catggagatc gtccaggagc ggctggagcg ggagttcgac
     4261 ctcgacctga tcgcctcggc gccatcggtc gagtaccacg tcaagctcct gtacggccgc
     4321 ggcgaggtct ccatcgacaa cccctcgctc ctgccgaacc cgtccgacat cgaggtcctc
     4381 gaggagccgt gggtcaacct cagcgtcgtg acgccatcga gctacatcgg acccctgatg
     4441 gagctctcga ccaaccgccg cggcctgttc cagaacatgg aatacctcga cccgacccgc
     4501 gtcgtgctcc acttcgagat gccactcgcc gagctgatca tcgactactt cgaccagctc
     4561 aagagccgca gccagggcta cgcgtcgatg gactacgagg agctcggcta ccgggcggcg
     4621 aacctcgcca aggtcgacat cctcgtcaac ggcgagccgg tcgacgcgct gtcgctgatc
     4681 gtccatcgcg acaaggccca gtcggccggg cgcgtcctca ccgaacgcct caagcagctg
     4741 atcccgcgcc agatgttcga gatcccgatc caggcggcga tcggaagcaa cgtcatcgcc
     4801 cgcgaaacgg tgcgggccat gcgcaagaac gtcctcgcca aatgctacgg tggcgacatc
     4861 acccggaagc ggaagctgct cgagaagcag aaggaaggca agcagcggat gaagcgggtc
     4921 ggatcggtcg agatcccgca ggaggccttc ctcgcgatgc tccggatgga cgagggctag
     4981 gagccggtgg agacctggct gccgacgatc cggacgctga tcgcgatcgg gctcgccctg
     5041 ctcctcgtca tgctccgcct cgaggcggtc cggttcgggg ccgcggaata cgacgatccg
     5101 gttgacggcc gggcgccctc ggtccgccgt cgggtggcct ggtacgccgt cgggatcctc
     5161 ctcgtcgtcg ggatcctcct cgttcacccg agggcacagg aggatttcgc gctcagcgcg
     5221 ggcgatcgct cgcaggcgtt cgtgtacggg ttcctgtacg cggcgctcgg gacggcgatc
     5281 gcgctcggga tcgcgttctg gcggtatcgt cggctgcgtt ttcccgacgt ctggtcgtac
     5341 cccggcgcgc tgctgaacgc cgtggtcacc gcgctcatcg acgaggtcgc atttcgcggc
     5401 gcggtcctgg gcctgctcct cctgaccggc ctcgagccga ccgcggcgaa tgcgatccag
     5461 gcgatcctct acgcgctcgc cacgcggctc ggagcgcccg gccggaaccg ctggatgctg
     5521 gtcatggcga tcctcgtcgg gcttgccggc ggctgggtga cggcggtgac cggcggcatc
     5581 gcggctgcgt tcatcggcca cgccgtgacc cgcttcgcgg tctttctctc gaccggccat
     5641 gccggccagt tcctgccgcg tggccgcgag gtcgaggaga tcgaggcgac acgacggccg
     5701 cccaagggct ggcgggtgat cgggtcgcga gacccgacat cgcgcgatcg atgatgccgc
     5761 agtgcgcgtc cgacgcccgc aagcgcgcga cgctgcccac gcgaacaagc actgcccacg
     5821 cgaacaagcc actgcccacg cgaacaagcc actgcccacg cgtttcggat agccgaatga
     5881 ttcgaggttc gcagtattcg gctattcgca agacgggcgc gggcatccgt tgccgtccac
     5941 gcgggccagg caccaaatga cggcgatggg cgcggcacgc gacgccacgc cgcccggccg
     6001 ggacggcctg ctcacgccgc gcccgccggc cgggctgtat ctccacatcc cgttctgcgt
     6061 atcgctctgc ccgtactgcg acttcgtcgt cgtcgtcggc gcggccgcac gcgggccggc
     6121 gaaccggatc ggggccctgg cggccgcgct ccggacggag ctcgagcttc gcgccgacac
     6181 cctcgacgct gcctacggga agcccgggtc cgcgagccgg ccggcgctcg acaccgttta
     6241 catcggcggc ggcacgccga ccctcgtgcc ggccgacatc gtggcgaccc tgctcgagac
     6301 ggtggaccgg cgttacggta tcgcccccga tgccgaggtc acgatcgagg ccaaccccgg
     6361 ccccgacgag cggggtgacg ccgcggccct cgcgcgggcc ggcatcaacc ggatctcctt
     6421 cggcgcgcag agcctcgatg ccggtgagct ccgtcggctc ggccgtcgcc accgaccgtc
     6481 cgacgtcgcc gacgccgttg cgggcgcccg agcaggcggc atcggatcgg tgaacctcga
     6541 cctcctctac gacgtccccg acggatcgct ggccacctgg atcgagacgc tcgagcaggc
     6601 cctcgcgctc gagcccgacc atctctccct gtatgccttg accctcgacg atcccgacgc
     6661 ggagggactg accggggccg acggcgacca cctgccgacg acccgcggtg cccgccgttg
     6721 gcgcgacgcg gcgatcccgg cccaggacga ggatcgcgcg gccggccagt accaccacgc
     6781 cgtccatcgc ctggcggccg acggctggcg cggctacgag atcagcaact gggcccggcc
     6841 cggccacgag agccgccaca acctcgccta ctgggagcgc cggccgtacg aggccgtcgg
     6901 gccgggcgcc cacgcgttcg acggcgcgac gcggcgctgg aacgcggcga acctggcgcg
     6961 gtacctcatg gccctggcgc cggccggcga cgaaccggcg cgcctgccgc cgggcggcgc
     7021 ggagaccctc gacgccgcga ccgccctcgc cgaatcgatc gtcctcgggc tgcgaaccga
     7081 ccgtggcctg cctcgtgcgg ccgcgaccaa accgccgctg gccgatgtct tcggctgggc
     7141 actggcggcc gagctgctga cgatcgacga cggcgaccgg atcgtgctca cgacccgtgg
     7201 ccggctgctc tcgaacgagc tcttcgcgcg cctcgtctag tcgcccgccg gcgccgacga
     7261 cgggcaaagg ccgtctcaac ccgttgacac tccgaacgcc ggactgctag catccttagc
     7321 actcagcacc caagagtgct aactcgcgaa ccctgcggcg agcccgtcgc cgcacgcacc
     7381 gaggcagcag cgtggcgcgc cgtacccgca ccagtggctc cctggatcct cgcgccgagt
     7441 cgatcctccg ggccgtcatc gacgaatatg tctcgaccgc cgttccggtc ggatcccact
     7501 cgctcgtcga gcgctacggc ctcggcgtgt cgagcgcgac cgtccggaac atcctggccg
     7561 cgctcgaatt caacggcctg ttgacccatc cccacacgag cgcgggccgg atcccgacgg
     7621 acgccggcta ccgccacttc gtcgagtcga tctcgagcgc gagccagctc ccgcaggtcg
     7681 agcagctgat gatccgccac cagttcggcc aggtgcagca cgcgtcggag cagtggttcc
     7741 gtctcgcggc cacgaccctc gccacggcca accggacggc cggactcgcg acccaggcca
     7801 agccgcgcgc ggcccacgtc cggcgactcg acctcgtctc gatcagcgac cggctcgcca
     7861 gcctgatcat cgtcttccgc gagggtgccc ttcgccaggt cctcgtcacc ctcgccgagc
     7921 ccgtcgagca ggaggacctg acccgcgccg cggtcatcct cgacggcctg atggccggcc
     7981 ggaccgcggt cgacgccgag cgggccctcg cccggatcga cggcgacggc ccgatcggga
     8041 cgctcgtccg gcgggtcggc gagcgggtgc tcgccgccgt ccgcgactat gattcgaccg
     8101 gcatcgagga ggtcttcagc gatggcctgc tccatgtcat ggaggcgccg gaattcgagc
     8161 ggagcgacaa gctccggcgc gtcttcgccg ccctcgagaa cagggcctat ctcggcgagc
     8221 tactcgaccg ggtcgcgggc accggcacgg tccaggtctt catcggcgcc gagaatgcgc
     8281 ccgaggagat gcgcgacgtg tcgctcgtcc tcgcccccta cggtgagccg ggccgggcgg
     8341 tcggcgtggt cggcgtcctc ggtccgaccc ggatggccta tccccaggcg atcggcacgg
     8401 tccggttcgt ctccaccctc atgaacgaac tggtgcagca catctatgcc tgacccgcgc
     8461 aacttccggg gccggcccca cggcggcccc agcgaacccc atcccggagg cccccgcatg
     8521 ccccat
//
LOCUS       DATAIW010000272         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_245653, whole genome shotgun sequence.
ACCESSION   DATAIW010000272 DATAIW010000000
VERSION     DATAIW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245653"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VHM48_08615"
     CDS             complement(<1..611)
                     /locus_tag="VHM48_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755512.1"
                     /translation="MDRIPFLGETLVRLSLDADEVQPGPLQPSIARAIVGRLNADRAP
                     GSRQPLPGELLALSLLHEAAHLAIVEVARRQPEAAISAALPTVRAAVGVRRAKRLLED
                     FASSFPGVDRPAPARLEDLLLVHLANDNPAAAPLRELVDDRGLPAPSLTAAMRAIERH
                     QAGFALEGEGPGKGVSLIELLREPAHRAPASLAGQLRYVREHWA"
     gene            complement(682..>1575)
                     /locus_tag="VHM48_08620"
     CDS             complement(682..>1575)
                     /locus_tag="VHM48_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2755513.1"
                     /translation="RLLPGARLAPVREMGAALRVNPNTIRAVYRRLADAGYVTSRHGA
                     GTHVADRPPQRRGAEALAGIVSEMLRRAAQAGFGADEVAAAAFAAATERKRPGPKVQV
                     LFAECTNADAGYDAARLGDAFPDLIDVEGALLDDLPDRLDRFHYDLVATTTFHADEAQ
                     ALVHARVPVVAMLVGPGYLELVHEISGLRAGTRVGLVCASERGAENIAETLSLSGTTG
                     VELISATMEDNRAIELIDRTADVILLSREAIANGLDQRFERPERVRRWSYEFDPAGLE
                     LLRRAIEHVMTSRRREPVAAG"
BASE COUNT          211 a          555 c          602 g          207 t
ORIGIN      
        1 gcccagtgct cgcggacgta gcgcagctgg ccggcgagcg aagccggggc gcggtgggcg
       61 ggctcccgca gtagctcgat cagcgacacg cccttcccgg gaccctcgcc ctcaagggcg
      121 aatcccgcct ggtggcgctc gatggcccgc atcgcggccg tcaggctcgg cgccggcagg
      181 ccgcggtcgt cgacgagctc ccgcagcggc gcggccgccg ggttgtcgtt ggcgagatgg
      241 acgaggagga ggtcctcgag acgggcgggc gccgggcgat cgacccccgg gaacgatgag
      301 gcgaagtcct ccaacagtcg cttggctcgg cggaccccga cggcggcacg gacggtcggg
      361 agcgcagccg agattgccgc ctcgggttgt cgccgtgcca cctccacgat cgccagatgg
      421 gcggcctcgt ggagcaggct cagggcgagc agctcgccgg gaagcggctg gcgcgatccg
      481 ggcgcgcggt ccgcattgag gcgcccgacg atcgcccgcg cgatcgacgg ttggagcggt
      541 cccggctgga cctcgtcggc gtcgaggctc agccggacga gcgtctcgcc gaggaacggg
      601 attcggtcca tgtcccgccg attctggcac gtctggctcg atccggaagg tgcgccatgt
      661 cggccgccgg cggcgcgggc gtcagccggc ggcgacgggc tcccgacggc gcgacgtcat
      721 cacgtgctcg atcgcccgac ggagcagctc caagccggcc ggatcgaact cgtacgacca
      781 gcggcgaacc cgctccggtc gctcgaagcg ctggtcgagc ccgttggcga tcgcctcccg
      841 cgacagcagg atgacgtccg cggtccggtc gatgagctcg atcgcccggt tgtcctccat
      901 cgtcgccgag atcagctcga ccccggtcgt cccggacagc gacagcgtct cggcgatgtt
      961 ctccgcgccg cgttccgacg cgcagacgag gccgacccgt gttccggccc gcagccccga
     1021 gatctcgtgg acgagctcga gatagccagg gccgacgagc atcgcgacga cgggcacccg
     1081 ggcgtgcacg agggcctggg cctcgtcggc gtggaaggtg gtcgtcgcga ccaggtcgta
     1141 gtggaagcga tcgagccggt cgggcaggtc gtcgagcagg gcgccctcga cgtcgatgag
     1201 atccgggaac gcgtcgccga ggcgggcggc atcgtagccg gcgtcggcgt tcgtgcactc
     1261 ggcgaagagg acctggacct tggggcccgg ccgcttccgt tccgtggccg cggcgaaggc
     1321 ggccgcggcg acctcgtcgg ctccgaaccc ggcctgggcc gcccggcgga gcatctcgga
     1381 cacgatcccg gcgagggcct ccgcgccgcg gcgctggggt gggcgatccg cgacatgcgt
     1441 cccggctccg tggcgactcg tgacgtagcc cgcgtcggcg aggcgccgat acaccgcccg
     1501 gatcgtgttg gggttcactc gcagggcggc tcccatctcc cggacgggag caagtcgcgc
     1561 accgggcagg agccg
//
LOCUS       DATAIW010000273         6551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_186231, whole genome shotgun sequence.
ACCESSION   DATAIW010000273 DATAIW010000000
VERSION     DATAIW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186231"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="VHM48_08625"
     CDS             complement(<1..2241)
                     /locus_tag="VHM48_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755514.1"
                     /translation="MTDEPIERALRDWLADHTEPDVPAGLRRHLDGLVDGGVPGAGGR
                     TAVQPVRRLVPTLATTRRGWAQAIAAGVAVALVGGGLVYGLTLRPGPAVSGSPTPGAS
                     RAPHGSASPTIAESTSVVDAGLVDPANGWALTSSDLVWTADGGATWRSIRPSDVAASS
                     IRAVRFVDARMGWLAWWSEADPRVTIERTSDGGQTWSRSHTPGDHPDGIGRVSIEAFD
                     DGTLYVQVESVHSSASCVGELDASTDSGISWLPDVGFGAFDAGCAPIGFRDHVHGWTT
                     AGPLNQILIATSDGGRTWSQVYLRNPRSYGLNIPAFQLPVFADSGRGLDGVLSVRLSP
                     PPDFNSAGRSGNLEIDRTADGGSTWQPVSGPPDPNLPFEGAIAIATLSAADWLVGGSS
                     LGSRAWITSDAGGSWTAIAAAGIDSPIASLRFVDATTGWAIDRDGALHATADAGRTWR
                     TLDPRPTALAEPSPSVPPVTGSGPFTWTPVSSEGELATYDVVQAIHRRNGGSIGIAFG
                     QEARTVHSDDGMTWILDPADPGLVAAATDHTNIVLGIADGPAGLVVVGAAANGDSSAG
                     DVRAWRSTDGLKWQAASQIGGDLNAEMRAVIGGKQGYVAVGSDGFPGASALESGSRGA
                     AVWTSADGSSWARVADQPAFLGSTMTGITATSTGYVAWGGNLPMVEVRATQPPIWTSS
                     DGRTWLRANGSIFARDVFFPLGGIVIEPERWIALGTRRGSAAEGGGFLPGAWSSTDEG
                     RSWTATQ"
     gene            complement(2238..2807)
                     /locus_tag="VHM48_08630"
     CDS             complement(2238..2807)
                     /locus_tag="VHM48_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2755515.1"
                     /translation="MARGSDDEPAAPVDPATPDDVARRAFHRLAGSSLAGSYRLAVHL
                     VGDRSDAEDVVQEALTLAWRGWPSLRDHDRFEAWFERIVVNTAYERLRKRRRKPTAHL
                     PDEVVDRGADRLAAAIDRDAIGRALDGLSPDHRVVVILRYWRDLPIEAIAERLGVPSG
                     TVRSRLHYALLELRRAIDPPLLRPEEARP"
     gene            2989..3336
                     /locus_tag="VHM48_08635"
     CDS             2989..3336
                     /locus_tag="VHM48_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755516.1"
                     /translation="MGALQTLDATTSAPASAVIWLNGRQAIIAGIRGDEQVSQVIVNR
                     GPGTELEYLAAVVRAIGDRERVMILGPNQVRLDLEREYVSIYRRPERLVDVEPAGRMD
                     AEALVEWVRRLAS"
     gene            3534..4877
                     /locus_tag="VHM48_08640"
     CDS             3534..4877
                     /locus_tag="VHM48_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX2755517.1"
                     /translation="MDRSRIIIMGAAGRDFHDFNVVYRSDPTVEVVAFTAAQIPGIAD
                     RRYPPELAGPSYPHGIPIVAERELEDLIRDQRVDTVVFAYSDVSHETVLRAASRVLAA
                     GADFTLLGPDRTMLKSTRPVLAVGATRTGAGKSQTTRYLASLLADAGLTPVVVRHPMP
                     YGNLVAQRVERFATLADLDRFDTTIEEREEYEPHIDAGRVVYAGVDYEAILRQAEMEA
                     DVILWDGGNNDFPFYRPDLFIVVADPLRPGDERHYHPGEANVRMADAVIINKIDSAEP
                     EAVAAVRATIHEFNPRAEILTARSDLTLVGPPIAGKRVVVVEDGPTLTHGGMRYGAGV
                     VAARRFGAASMADPRPAAVGSIREVLDRYPALEPLVPAMGYGPAQISELEATLNAVDA
                     DIVLSATPIDLTRVLSLNKPITRVRYDLVEAGGKPLAEVIEPILGLARTPVLAGA"
     gene            4882..5868
                     /gene="argF"
                     /locus_tag="VHM48_08645"
     CDS             4882..5868
                     /gene="argF"
                     /locus_tag="VHM48_08645"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2755518.1"
                     /translation="MNTTLAAVPPSSTDAAPRHFLTLDGLGSGGLRSLLDLTGRLKRD
                     PDAFRNRLAGGRIGMIFDKPSTRTRVSFESAAWMLGMLPIVLRPDELQLGHGETIADT
                     ARALSLYLDAITVRTFDQAHVEELAAAASIPIVNALTNEHHPCQALADVMTIEEEFGT
                     LAGIRLAFVGDGDNVCHSLIQAAGLGGFELRIATPPGYEPSPEVVAAARLNGRRTGGS
                     IKLMNEPIEAVRGADAVYADVWTSMGHETEREARMAAFAGFRVDAALMERASASAIFL
                     HCLPAHRGEEVTDEVIDGPASRVWPQAANRLHTETALLYSVLTGDAEGDRLG"
     gene            complement(5936..>6551)
                     /locus_tag="VHM48_08650"
     CDS             complement(5936..>6551)
                     /locus_tag="VHM48_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2755519.1"
                     /translation="CTSDATTQGLATRPAYEAHSEAWLLLCEAEATRLHRRADPDAWV
                     ACVLAWEHLRRPYPVAYASWREAEARLAARGDPALAETALRTAHRIAELLRAEPLARE
                     VVALATRARLTLETSEPGQAARTPAAPDDEARLGLTAREREVLTLVALGRTNRQIAAE
                     LFISENTAGVHVSNILGKLAVTGRGEAAAVAYRLGLVEPASEPA"
BASE COUNT          943 a         2345 c         2348 g          915 t
ORIGIN      
        1 ctgcgtggcc gtccacgaac ggccctcgtc ggtcgaggac caggcccccg gcaggaaccc
       61 gccgccctcg gcggcgcttc cccgacgggt gccgagcgcg atccatcgct cgggctcgat
      121 gacgatcccg ccgagcggga agaacacgtc gcgagcgaag atcgagccgt tcgcccgcag
      181 ccacgttcga ccgtcgctcg acgtccagat cggcggctgc gtcgcgcgga cctcgaccat
      241 cggcaggttc ccaccccacg cgacgtagcc ggtcgaagtc gcggtgatac cggtcatcgt
      301 tgacccgagg aacgcgggct gatccgcgac cctcgcccac gacgacccgt ccgcggacgt
      361 ccagaccgcg gcgccgcgcg agccgctctc gagcgcgctc gcgcccggga agccgtcgct
      421 cccgaccgcg acgtagccct gcttgccgcc gatgaccgcc cgcatctcgg cgttcagatc
      481 accgccgatc tgggaagccg cctgccattt caggccgtcg gtcgaccgcc acgctcggac
      541 gtctcccgcg ctgctgtcgc cattggcggc cgcgccgacg acgacgagcc cggccggccc
      601 atcggcgatc ccgagcacga tgttggtgtg gtcggtggcc gcagcaacga gaccgggatc
      661 ggccggatcg aggatccagg tcatgccgtc atcggaatga accgtccgag cctcctggcc
      721 gaacgcgatc ccgatcgagc cgccattccg ccggtggatc gcctggacga cgtcgtacgt
      781 cgcgagctcg ccctccgagg agacgggcgt ccaggtgaac gggccgcttc cggtcacggg
      841 tgggaccgag ggcgaaggtt cggcaagggc cgtcgggcgc gggtcgaggg tccgccaggt
      901 ccggccggca tcggcggtcg cgtggagcgc tccgtcgcga tcgatcgccc agccggtcgt
      961 cgcgtcgacg aaccggagcg aggcgatcgg tgaatcgatg ccggccgcgg cgatcgcggt
     1021 ccacgaaccg cccgcgtccg acgtgatcca ggcgcgtgag ccaaggctcg aaccgcccac
     1081 gagccagtcg gcggccgaga gggtcgcgat cgcgatcgcg ccttcgaacg ggaggttcgg
     1141 gtccggcgga cccgagaccg gctgccaggt cgagccgcca tccgccgtgc gatcgatctc
     1201 gaggttgccg gatcgtccgg cactgttgaa gtccggcggc ggcgacaggc gcaccgatag
     1261 gaccccgtcg agaccgcggc cagagtcggc gaagacgggg agctggaagg ccgggatgtt
     1321 gaggccgtag ctgcgcgggt tccggaggta cacctggctc caggtgcgtc cgccgtccga
     1381 ggtcgcgatc aggatctggt tcagcggtcc tgcggtcgtc cagccgtgga cgtggtcgcg
     1441 gaacccgatc ggagcgcatc ccgcgtcaaa cgcaccgaac ccgacgtcgg gcagccagga
     1501 gatgcccgaa tcggtgctcg cgtccagctc gccgacgcac gaggcggaac tgtggaccga
     1561 ctcgacctgg acgtagagtg tcccgtcgtc gaaggcctcg atcgagaccc gtccgatgcc
     1621 gtcggggtgg tccccgggcg tgtgcgatcg gctccatgtc tgcccgccgt cgctcgtccg
     1681 ctcgatcgtg acccgcgggt ccgcctcgga ccaccatgcg agccagccca tccgggcgtc
     1741 gacgaaacgg accgcacgga tcgatgacgc ggccacgtcg gacgggcgga tcgaccgcca
     1801 ggtcgcgccg ccatcggcgg tccacacgag gtcgctcgac gtcagggccc agccgttcgc
     1861 cggatcgacg agcccggcgt cgacgaccga cgtcgactcg gcgatcgtcg gcgaggccga
     1921 gccgtgcggt gccctggacg cgccgggcgt cggcgagccg ctgaccgcgg ggcccggtcg
     1981 cagggtcagg ccgtagacca gcccgccccc gaccagggcg acggcgactc ccgcggcgat
     2041 cgcctgcgcc catccccgtc gcgtggtcgc gagggtcggc acgagacggc ggaccggctg
     2101 aacggcggtc cgcccgccgg cgcccgggac gccgccgtcg accagcccgt cgagatgccg
     2161 ccgcaggccg gcggggacgt cgggctcggt gtggtcggcc agccagtccc ggagggctcg
     2221 ctcgatcggt tcgtcggtca cggccgagcc tcctcgggtc ggagcagcgg tggatcgatc
     2281 gcccggcgca gctcgagcag ggcgtagtgg aggcgcgaac ggaccgttcc ggatgggacg
     2341 ccgagccgct cggcgatggc ctcgatcggt aggtcgcgcc agtagcggag gatgacgacg
     2401 acgcggtggt cggggctcag cccgtcgagc gcccggccga tcgcgtcgcg atcgatcgcg
     2461 gcggccaggc ggtcggcgcc gcggtcgacg acctcgtcgg ggaggtgggc ggtcggcttg
     2521 cggcgccgtt tgcggagacg ctcgtacgcc gtgttgacga cgattcgctc gaaccacgcc
     2581 tcgaagcgat catggtcgcg caggctcggc cagcctcgcc aggcgagggt cagcgcttcc
     2641 tggacgacgt cctccgcgtc ggatcgatcg ccaacgaggt gaacggcgag ccggtacgac
     2701 cccgcgagcg acgagccggc cagccggtgg aaggcgcgcc gggcgacgtc gtccggggtc
     2761 gcgggatcga ccggtgcggc gggttcgtcg tcggagccgc gggccacatc acccccagga
     2821 tcgttcggct cggggagccg ctgccgattc tgacggtgga gaccacccgg atgttcacgt
     2881 gatcgagatg cggtgctcaa cgtcgggtcg ggcctcgatt tcgagccgat cggcagtgtc
     2941 gcggtggctc ccgaccggcc tacgctcgat ggaagaagga gtcgggacat gggcgcactt
     3001 cagacactcg atgccacgac atcggcgccg gcgagcgccg tcatctggct caatggtcgg
     3061 caggccatca tcgccggcat ccgcggcgac gagcaggtct cgcaggtcat cgtcaaccgt
     3121 ggccccggaa cggagctcga gtacctcgcc gccgtcgttc gggcgatcgg cgatcgcgag
     3181 cgggtgatga tcctgggccc gaaccaggtc cggctcgacc tcgaacgcga atacgtgtcg
     3241 atctaccggc gaccggagcg gctggtcgat gtcgagcccg ccggccggat ggacgccgaa
     3301 gcgctcgtcg aatgggtacg gcgtctggcc tcctgactga ctgatgtcgc gggtcgcggc
     3361 agtcggcgtt ggcaacacgc gcgttccgcg ggtgccgcgc catgtccaat gaccgccgtc
     3421 cgcgggccga agagcactgt cccctcgaca gcgttgtcgg ccacggtgat cgtgacgggt
     3481 tgtcgctgct ggcatggcgc acgttcggcc ggcagcccgg acaggaggcc acgatggacc
     3541 gttcgcggat catcatcatg ggcgcagccg ggcgcgactt ccacgacttc aacgtcgtct
     3601 atcgctccga tcccacggtc gaggtcgtcg ccttcacggc ggcgcagatc ccggggatcg
     3661 ccgatcgtcg ctatccgccg gagctggcag gccccagcta tccgcacggc atcccgatcg
     3721 tggccgagcg cgagctcgag gacctcatcc gcgaccagcg cgtcgacacc gtcgtcttcg
     3781 cctacagcga cgtcagccac gagacagtgc tgcgggcagc gtcccgcgtc ctggccgcgg
     3841 gcgcggactt cacgctgctc ggaccggacc ggacgatgtt gaagagcact cgaccggtcc
     3901 tcgccgtcgg ggcgacccga accggcgcgg ggaagagcca gacgacccgc tacctggcga
     3961 gcctgctcgc cgacgccggc ctcacgccgg tcgtcgtccg ccatccgatg ccctacggca
     4021 acctggtggc gcaacgggtc gagcggttcg cgaccctggc cgacctcgac cggttcgaca
     4081 cgaccatcga ggagcgcgag gagtacgagc cgcacatcga tgccggtcgc gtcgtctatg
     4141 ccggcgtcga ctacgaggcc atcctgcgcc aggccgagat ggaagccgac gtgatcctgt
     4201 gggacggcgg caacaacgac ttcccgttct atcgcccgga cctgttcatc gtggtcgcgg
     4261 acccgctgcg tccgggcgac gaacgccact accatccggg cgaggcgaat gtccggatgg
     4321 ccgacgccgt gatcatcaac aagatcgact cggccgaacc cgaggccgtc gccgcggtcc
     4381 gggcgacgat ccatgagttc aacccgcgag ccgagatcct cacggcgcgg tccgacctga
     4441 ccctggtcgg gccgccgatc gccggaaagc gggtcgtcgt cgtcgaagac ggcccgacgc
     4501 tcacccacgg cgggatgcgc tatggcgccg gcgtcgtcgc ggctcgtcga ttcggcgcgg
     4561 cgtcgatggc cgatccgcgg ccggccgccg tcggctcgat ccgggaagtc ctcgatcggt
     4621 atccggccct cgagccgctc gttccggcga tgggctacgg ccccgcccag atcagcgagc
     4681 tcgaggcgac cctgaacgcc gtcgacgcgg acatcgtcct gtcggcgacg ccgatcgatc
     4741 tgaccagggt cctcagcctg aacaagccga tcacgagggt ccgctatgac ctcgtcgaag
     4801 cgggcggcaa accgctcgcc gaagtcatcg agccgatcct cgggctggcc cggacgccgg
     4861 tcctcgcggg agcctgatgc catgaacacg acgctcgccg cggtgccgcc gtcgtccacg
     4921 gacgcggcgc cccgccactt cctcaccctc gacgggctcg ggtccggagg cctgcgctcg
     4981 ttgctcgatc tgaccggccg gctcaagcgc gacccggacg cgttccgcaa ccgcctggcc
     5041 ggcggtcgga tcgggatgat cttcgacaag ccgtcaaccc ggacacgggt cagcttcgag
     5101 tcggcagcgt ggatgctcgg gatgctgccg atcgtcctcc gccccgatga gctccagctc
     5161 ggtcacggcg agacgatcgc cgacacggcc cgggccctgt cgctgtatct cgacgcgatt
     5221 acggtccgga ccttcgacca ggctcacgtc gaggaactcg cggcggccgc gtcgatcccg
     5281 atcgtcaacg ccctcaccaa cgagcatcac ccctgccagg ccctggccga cgtgatgacg
     5341 atcgaggagg agttcgggac gctggccggc atccggctgg ccttcgtcgg cgacggcgac
     5401 aacgtctgcc actcgctgat ccaggcggcg ggcctcggcg gcttcgagct ccggatcgcc
     5461 acgccgccgg gctacgagcc gtcgccggag gtcgtcgccg cggcgcggct caacggccgg
     5521 cgaaccggcg ggtcgatcaa gctgatgaac gagccgatcg aggccgtccg cggcgccgat
     5581 gccgtctacg ccgatgtctg gacctcgatg ggccacgaga ccgagcggga ggcccggatg
     5641 gccgccttcg ccggcttcag ggtcgacgca gccctgatgg agcgtgcctc ggcctcggcg
     5701 atcttcctcc actgcctgcc cgcccatcgc ggcgaggagg tcaccgacga ggtgatcgac
     5761 ggaccggctt cccgcgtctg gcctcaggcc gccaaccggc tccacaccga gaccgctctg
     5821 ctctactccg tgctgaccgg agacgcggag ggcgatcggc tgggctgaac cgcggctccc
     5881 ggggccgggg aacgctccgc gcaacgggga agcccgaggc ctctgaccga cgtcgtcagg
     5941 caggttcgct ggccggctcg accaggccga gccggtaggc caccgcggcc gcctcgccgc
     6001 gcccggtgac cgcgagcttg ccgaggatgt tcgagacgtg gaccccggcc gtgttctcgc
     6061 tgatgaacag ctcggccgcg atctggcggt tcgtgcggcc gagggccacg agcgtcagca
     6121 cctcgcgctc gcgcgcggtc aggccgaggc gtgcctcgtc gtcgggtgcg gccggcgtcc
     6181 tggcggcctg gcccggctcg ctggtttcga gggtcagccg cgctcgcgtt gccagggcca
     6241 cgacctcgcg ggccagcggc tccgcccgca acagctccgc gatcctgtgg gcggtccgca
     6301 gcgccgtctc ggccagggcc gggtcgccgc gggccgcaag gcgcgcctcg gcctcgcgcc
     6361 aggaggcata ggcgacgggg tacgggcggc gaaggtgttc ccatgccagg acgcaggcga
     6421 cccacgcgtc ggggtcggcc cggcggtgaa gacgcgtcgc ctcggcctcg cacagcagga
     6481 gccacgcttc ggagtgcgcc tcgtacgcgg gccgcgtcgc gaggccttgg gtggttgcgt
     6541 ccgaggtaca g
//
LOCUS       DATAIW010000274         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_166352, whole genome shotgun sequence.
ACCESSION   DATAIW010000274 DATAIW010000000
VERSION     DATAIW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166352"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..297)
                     /locus_tag="VHM48_08655"
     CDS             complement(46..297)
                     /locus_tag="VHM48_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755520.1"
                     /translation="MTDPIQSGHSSDDAAAAARAAGLDCLRCGAAIRLDSVQAFRTGG
                     STGATHFFLGQWAEIGEDKVTLEAYVCGQCRHVEFRVPE"
     gene            complement(294..>2186)
                     /locus_tag="VHM48_08660"
     CDS             complement(294..>2186)
                     /locus_tag="VHM48_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2755521.1"
                     /translation="WCHVMERESFEDAATAADLNRDFVAIKVDREERPDLDQVYMGAV
                     QAMTGQGGWPMSVFLTPDHQPFYGGTYFPPQPHHGLPAFRDVLAGAARAWREQRLEVV
                     AAGQRIVDSLVSQNRLPTDGPAPTAELLDAAVDALQGRFDTLNGSWGGAPKFPQPMTL
                     DFLLARIAAGDGRSRTMVRYTLDRMADGGIHDQLGGGFHRYATDPIWLVPHFEQMLYD
                     NAQLARTYLRAWATLGIESYRDVAFGVLEYLLRELRRSDGTFAASQDADTDGVEGATF
                     TWTAAEIAGLLAGDALLFATTYGVRPEGNWEGRTILSRIQPAAGDDGIVDADREARLA
                     DARGRLMAVRAQRPQPARDDKALAAWNGLAIGAFADAARLLPGAEGERYRTAAVDAAS
                     AILAGLRRPDGRLGRSWKDDRATGEGVLEDYADLADGLLALYEATFDERWFVAARDLA
                     DAILDHFVDPVGGFFDTADDHESLVTRPKDPQDNATPAGGSMATRVLLRLAALTADHR
                     YHEAAERALATVTPYLERYPTGFANWLSAAALSVGGIVELAIVGDPDDPATRALLATA
                     WSGGRADLVVAVSGDPAASVVPVLADRVAIDGRPTAYVCHDFACRLPVTDPTHLSDQL
                     AEGRPR"
BASE COUNT          317 a          776 c          794 g          299 t
ORIGIN      
        1 gcgcatcccg ggcgccgccg tcatcggtcg ggggcaccgg ctcggtcatt ccggaacacg
       61 aaactcgacg tgacgacact ggccgcagac gtacgcctcg agggtcacct tgtcctcgcc
      121 gatctcggcc cattgcccaa ggaagaagtg cgtcgccccg gtgctgccgc ccgtgcggaa
      181 cgcctggacg ctgtcgagcc tgatcgcggc gccacaacgg aggcaatcga gcccggcggc
      241 ccgcgccgcc gcggcagcgt cgtcggacga atgtcctgac tggatcgggt ccgtcatcgt
      301 ggtcttcctt ccgcgagctg gtcactgagg tgggtcggat cggtgaccgg cagccgacac
      361 gcgaagtcat ggcagacata ggcggtcggc cggccgtcga tcgccacccg gtcggcgagg
      421 accggcacga ccgaagcggc cggatcgccg gacacggcga cgaccaggtc agcgcggccg
      481 cccgaccatg ccgtcgcgag cagggcccga gtcgctgggt cgtccgggtc accgacgatc
      541 gcgagctcga cgatcccgcc gaccgacagc gccgcagcgg acagccagtt ggcgaacccg
      601 gtcggatacc gctcgagata cggcgtcacg gtggccagcg cgcgttccgc cgcctcgtgg
      661 tagcggtggt cggcggtgag cgcagccagc cggagcagga cgcgggtcgc catcgagccg
      721 cccgccggcg tcgcgttgtc ctgcgggtcc ttcggccggg tgacgagcga ctcatggtcg
      781 tcggccgtgt cgaagaaccc gccgaccggg tcgacaaaat ggtcgaggat cgcgtcggcg
      841 aggtcccggg cggccacgaa ccagcgctcg tcgaaggtcg cctcgtacag ggcgagcagg
      901 ccgtcggcga ggtcggcgta gtcctcgagg accccctccc ccgtcgcgcg atcgtccttc
      961 cacgatcggc ccagccgacc gtccgggcgg cggaggccgg cgaggatcgc cgacgcggca
     1021 tcgaccgcag cagttcggta gcgctcgcct tcggcaccgg gcaggagccg cgccgcgtcg
     1081 gcgaaggctc cgatcgccaa cccgttccag gccgcgagtg ccttgtcgtc gcgcgcgggc
     1141 tggggccgct gcgcccgaac ggccatgagc cgcccgcggg cgtcggccag ccgggcctcg
     1201 cgatcggcgt cgacgatgcc gtcgtcgcct gcggcgggtt ggatccgcga caggatcgtc
     1261 cggccctccc agttgccctc ggggcggacg ccgtacgtcg tggcgaagag cagtgcgtcg
     1321 ccggcgagca atcccgcgat ctcggccgcc gtccaggtga acgttgcgcc ctcgaccccg
     1381 tcggtgtccg cgtcctggct cgcggcgaag gtgccgtccg aacgccgcag ctcccgcaac
     1441 aggtactcca gcaccccgaa cgcgacgtcg cggtaggact cgatcccgag ggtcgcccac
     1501 gcccgaaggt acgtccgggc gagctgggca ttgtcgtaga gcatctgctc gaaatgcggg
     1561 acgagccaga tcgggtcggt cgcgtagcga tggaagccac cgccgagctg atcgtggatc
     1621 ccgccgtcgg ccatccgatc gagcgtgtaa cggaccatcg tccgcgatcg cccgtcgccg
     1681 gccgcgatcc gggcgaggag gaagtcgagc gtcatcggtt gggggaactt cggcgccccg
     1741 ccccagctgc cgttgagcgt gtcgaagcgg ccctggagcg cgtcgaccgc ggcgtcgagc
     1801 agctcggccg tcggagccgg tccgtcggtc ggcaatcgat tctgcgagac gagcgaatcg
     1861 acgatgcgct ggccggcggc cacgacctcg agccgctgct cgcgccacgc ccgggcggcg
     1921 ccggcgagga catcgcggaa cgccggcagg ccgtggtgcg gctggggcgg gaaatacgtc
     1981 ccgccgtaaa agggctggtg gtcgggcgtc aggaagacgc tcatcggcca gccgccctga
     2041 ccggtcatcg cctggacggc acccatgtag acctggtcga ggtcgggccg ctcctcgcgg
     2101 tcgaccttga tcgcgacgaa gtcccggttc aggtcggcgg ccgtggccgc gtcctcgaag
     2161 ctctcgcgct ccatgacgtg acacca
//
LOCUS       DATAIW010000275         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_106540, whole genome shotgun sequence.
ACCESSION   DATAIW010000275 DATAIW010000000
VERSION     DATAIW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106540"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHM48_08665"
     CDS             <1..501
                     /locus_tag="VHM48_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2755522.1"
                     /translation="TLTRVHGVDRRIDEMTPDELELLGVPTLEAVLTTISRRAFLDVE
                     LKGDPGRGAFEGLVAGRGPGLERAVVSSFEPAALERIAGLAPLWPRWLNATDAGSTTI
                     AAAVELGCRGISVEWHALDPAALARARSADLAVAAWTVRRRPTFARLERIGVAAICVE
                     AAALDG"
     gene            complement(509..1162)
                     /locus_tag="VHM48_08670"
     CDS             complement(509..1162)
                     /locus_tag="VHM48_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755523.1"
                     /translation="MTAIRRIRDVGAERAQRIIVELGRDIRDARRLAGLSQEQVARAA
                     GISHSHLSRIEHGRVPGISIELASRIAAAVGLDLSVRTFPGGIGIRDLGQVRLLGRFR
                     ALIAPSIKGRTEVPVGSVGDSRAWDDVLVANDRRAGVEAETHVRDWQALDRKIALRRR
                     DSGIQLVILVLADTRHHRAVLREIGPAGVTNFPEPARSALATLRAGSLPAASTIVLV"
     gene            1637..2887
                     /locus_tag="VHM48_08675"
     CDS             1637..2887
                     /locus_tag="VHM48_08675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2755524.1"
                     /translation="MANIESADLVVIGAGTVGGWASWFAKTSDAGRVVVLDRGLAGQG
                     ASSRAAGIVRAQGGTPTTVALGRWSIDFYRRQAAEIGTDSGFRELGYLILAVTEEDER
                     SGRERVAMQRNEGLAVRWLSATEAAALIPTLAPVGHRGGSHLETDGHIDPPRNVRAYS
                     LAMQHAGVELRERTTFTGLRTEPRPGGGSRVVGVETSAGPIATERVILTGGPGLRAVG
                     QAAGMRIPAGAVRHTVAVLEPDEAFAVERQSMVFDIGAGLYWRLEEGGLLFGWSDPND
                     QPGEARDIDWAMYDAMRTRLAGFVPVTRDLGLRRIWAATIDFTPDHLPILGPGLTAGG
                     ERIEGVTVASAGGHGMMWGPGVARVAADLAVHGRTDLIDVTDLGLDRFDAAGRSRLAT
                     DPIALPFPVSADERIARDAPVDGR"
     gene            complement(2903..3049)
                     /locus_tag="VHM48_08680"
     CDS             complement(2903..3049)
                     /locus_tag="VHM48_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755525.1"
                     /translation="MMIVTDHEREIRRPRYDSIVPRTSLAERIASALETMVRLGRPAT
                     TQPI"
     gene            3088..>3328
                     /locus_tag="VHM48_08685"
     CDS             3088..>3328
                     /locus_tag="VHM48_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755526.1"
                     /translation="MAVESFWATGGGFVVTVAASSGCPTSTSVANVMDIRRIRSDTAT
                     DPRRSPTIPVEAQQQPSAPQPQPPADASSIATARGR"
BASE COUNT          459 a         1183 c         1212 g          474 t
ORIGIN      
        1 acgctgacac gtgtccacgg cgtcgatcga cggatcgacg agatgacacc cgatgagctc
       61 gagctgctcg gggtgccgac cctcgaggcg gtcctcacca cgatctcgcg ccgcgcattc
      121 ctcgacgtcg agctcaaggg ggaccccggg cgcggcgcgt tcgaagggct cgtcgcgggt
      181 cgaggcccgg gtctcgagcg ggcggtcgtt tcgtcgttcg agccggccgc gctcgagcgg
      241 atcgccggac tcgccccgct ctggccgcgc tggctcaacg cgaccgacgc agggtcgacg
      301 accatcgccg ccgcggtcga gctcggctgt cgagggatct cggtcgagtg gcatgccctc
      361 gacccggccg cgctcgcccg agcccgatcc gccgacctcg cggtcgccgc atggacggtc
      421 cgccggcgcc cgacgttcgc ccggctcgag cggatcggcg tcgccgcgat ctgcgtggag
      481 gcggcagcgc tcgacggctg agcgtgcctc acacgagcac gatcgtgctc gccgcaggaa
      541 gcgatccggc gcgcagggtc gcgagggccg accgagcagg ttccgggaag ttcgtcaccc
      601 cagccggacc gatttcgcgc aggacagcgc gatggtgacg cgtatccgcg agcacgagga
      661 tgaccagctg tatccccgaa tcgcgccggc ggagggcgat cttccgatcg agggcctgcc
      721 aatcacggac atgcgtctcg gcctcgacgc cggcgcgacg gtcattcgcg acgaggacgt
      781 cgtcccaggc ccgcgaatcg ccgacgctcc cgaccgggac ctcggtgcgc cctttgatgc
      841 tcggcgcgat gagcgcgcga aaccgtccca ggaggcgaac ctggccgaga tcccggatcc
      901 cgattccacc aggaaatgtc cggacagata gatcgaggcc gacggcggcc gcgatccgtg
      961 acgccagctc gatcgagata cctggaacgc gtccatgctc gatccggctg aggtggctgt
     1021 gtgagatccc cgcggcgcgc gcgacttgct cctggctcag gcctgccagg cgccgcgcgt
     1081 ctcgaatgtc acgtccgagc tcgacgatga tccgctgtgc acgttccgcg ccaacgtctc
     1141 ggatccgtcg gatcgccgtc attcgacgat gatgaccacc gccccttcac caccgattac
     1201 ctcgcgatca tccgacgctc atcgcacggc gagggtctcc tcgatgggct ggtcgatctc
     1261 gggtgaggtg aaccaggacg gcgtgttgtg catccggcgt gcaactggac gacggaccgg
     1321 tgggctcact gctgtcgtag ccggtgttgt gcgtacggcg tgcaactcga cggccgatcg
     1381 cgcgcctccc cggtccggtt gcacccctgg cgcacaaccg agcccgcgcg aacccggatc
     1441 gcgcgcctcc ccggtccggt tgcacccttg gcgcacaacg gagcgcgcgg gtcgtcgagc
     1501 gtgcacgagt cacgcaggga gggccggccc gtgcccgcgc gccagcccgt gcgcgcgcgc
     1561 tggcccgcgc gatgcgccag cccgcgcgat gcgacgggcc gcgcgaccgg gccgcgcggc
     1621 cgcctatcct ccggacatgg cgaacatcga gagcgcggac ctcgtcgtca tcggggcggg
     1681 gacggtcggc ggatgggcga gctggttcgc gaagacgtcc gacgcgggcc gggtcgtcgt
     1741 cctcgatcgc ggtcttgccg gccagggtgc gagcagccgc gccgcgggca tcgtccgggc
     1801 ccagggcggg acgccgacaa cggtcgcgct cggccgctgg tcgatcgact tctaccgtcg
     1861 ccaggccgcc gagatcggga ccgacagcgg cttccgcgag ctcggctacc tgatcctcgc
     1921 cgtcaccgag gaggacgaac gatccggccg tgagcgggtc gcgatgcagc ggaacgaagg
     1981 cctggcggtt cgctggctgt cggcaacgga ggcagcggcc ctgatcccga ccctggcgcc
     2041 ggtcggccac cgcggtggca gccacctcga gaccgacggc cacatcgacc cgccgcgcaa
     2101 cgtccgggcg tactcgctgg cgatgcagca cgcgggcgtc gagctccgcg agcggaccac
     2161 gttcaccggt ctccggaccg agccgcgccc cggcggcggg agtcgcgtcg tcggggtcga
     2221 gacgagcgcc ggtccgatcg cgaccgaacg ggtcatcctg accggcggcc ccggcctgcg
     2281 cgccgtcggc caggcggccg ggatgcggat cccggcgggt gccgttcgcc acacggtcgc
     2341 ggtgctcgag cccgacgagg cgttcgcggt cgagcgccag tcgatggtgt tcgacatcgg
     2401 cgccggactc tactggcggc tcgaggaggg cggtcttctc ttcggctgga gcgacccgaa
     2461 cgaccagccc ggcgaggccc gcgacatcga ctgggcgatg tatgacgcga tgcggacgcg
     2521 gctcgccggg ttcgtgccgg tgacgcgcga tctcgggctc cgcaggatct gggccgcgac
     2581 gatcgacttt acgcccgacc acctgccgat cctcggcccg ggtctgacgg cgggcggcga
     2641 gcggatcgag ggcgtgaccg tcgcctcggc gggcggccac ggcatgatgt ggggccccgg
     2701 cgtcgcccgg gtggccgcgg atcttgcggt ccacggccgg accgacctga tcgacgtcac
     2761 cgacctcggc ctcgatcggt tcgacgccgc tggtcgcagc cgactcgcga ccgatccgat
     2821 cgcgctgccg tttccggtca gcgcggatga gcgaatcgcg cgggatgctc cggtcgacgg
     2881 acgctgaccg ggttcgcgcc gcctagatcg gctgggtggt tgcggggcgg cccaggcgga
     2941 ccatggtctc gagggcgctg gcgattcgct cggcgagcga cgtccggggc acgatcgagt
     3001 cgtagcgcgg ccgacggatc tcgcgctcgt ggtcggtgac gatcatcacg tagtaggcgg
     3061 cgatcggggt aggcggcgat cggggtcatg gcggtcgagt ccttctgggc gacgggtggc
     3121 gggttcgtgg tcaccgtagc cgcgagctcg ggttgcccga catcgacgtc ggtcgccaac
     3181 gtcatggaca tccgtcgcat ccggtcggac accgccaccg atccccgacg atccccgacg
     3241 atccccgtcg aggctcagca gcagccctcg gcgccgcagc cgcagccgcc ggccgatgca
     3301 tcgagcatcg cgacggcccg cggcagga
//
LOCUS       DATAIW010000276        11126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_47030, whole genome shotgun sequence.
ACCESSION   DATAIW010000276 DATAIW010000000
VERSION     DATAIW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47030"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VHM48_08690"
     CDS             <1..226
                     /locus_tag="VHM48_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755527.1"
                     /translation="EVEVIGEEGGSIRVRTPDGIEGWVPRVVIVGSPARERAPSASQP
                     EPPVARRRWSFPTLRRGSTTTDPAAESPRL"
     gene            complement(316..978)
                     /locus_tag="VHM48_08695"
     CDS             complement(316..978)
                     /locus_tag="VHM48_08695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2755528.1"
                     /translation="MSENDGESSATSPIAPTLDERAADPTCPVVDAASAERIQLVARQ
                     TWMVSIATFEVPHVVRPIAPPQGWSDPLTGADGPQLWDRIVLSEQARVGRYHRPVTVA
                     FAEIVGLDRLAGQWGWDVAERALAGCARRLGLEIRSSDHIARLEQARFGILLTETTEI
                     AAINFVERARASCEPELKAVGDDVTIGFGWASPPDKGDLSDAIDLALARLAAELEAGP
                     PD"
     gene            1135..3639
                     /locus_tag="VHM48_08700"
     CDS             1135..3639
                     /locus_tag="VHM48_08700"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX2755529.1"
                     /translation="MILSQAKEGRHTAMTIRTSPSRTTTPIAPQRSAPDALVVPRGAV
                     VPGRARPAAPLRREGPAKLTGEAKYADDLVFPGAWYGATIRSTDAHARFDGFELDPTL
                     DWSKIAVVTAEDIPGDNIVSLISDDQPVLVAVGGEIQHHAEPLLLLAAADREMLRHAR
                     HGVTIRSTPLPPVFDPLESDHVFAHYEIASGDLEQGFAGADYILEGEYRVGHQEQLYI
                     ENNAMIAVPREDGGVAVHGSLQCPYYIHKALKRALLLTSEQAQVVQAETGGGFGGKEE
                     YPSIVAIHAALLARKSGKPVRMIYDRHEDLAATTKRHPAIVTHRTGVKADGTLVAQDI
                     EVVMDGGAYCTLTPVVLSRGALHAGGPYRCANVRIRARATRTNTPPNGAFRGFGAPQV
                     EFAAEIQLGRVAEALGMSPLEIRRRNLYKVGDETPTGQVLRESVAAEEVLERAAEAAE
                     FEGVAARTKKARARRAGSGHVPSSPLRTSRDRTASGIGVALAWHGAGFTGSGEVKLAS
                     VASVELAADGRVRVLTGSTEMGQGTKTILPQLVAEELGLAAADVEIAPQDTAIVPDSG
                     PTVASRTTMVVGGLVIQAARRLRADVEARNGGAAFADVRVADAAAHGPTRVDQQFEPY
                     PHVHFDDETYRGDAYPAFGWAACVATVDVDLDSGEVTVRDVVAADDVGRVIHPILAAG
                     QVEGGTLQAIGYATIEEIKLDDGRYLNDRLATYIIPTALDAPRIHAILVEAPFSGAPH
                     GAKGVGELPMDVGAPAVVAAIHDATGVWIHELPASPERILAALGMTPSPDAPAPSPAA
                     TEAAVSDPIDGGTDPRPDAPTDPQPMRGPRDPGERR"
     gene            3650..4126
                     /locus_tag="VHM48_08705"
     CDS             3650..4126
                     /locus_tag="VHM48_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2755530.1"
                     /translation="MTTWHFTLNGDPVEIEAPGMRRLLDVLREELALTGTKEGCGEGE
                     CGACTVLLDGAPVDSCLVPVCQVDGARIATVEGLAARSPEGALLDPLQQAFLEAGGAQ
                     CGICTPGMLMAARAYLDAGGGPDEGAIRTAIAGNLCRCTGYTKIVDAIAIAAGGRR"
     gene            4123..4410
                     /locus_tag="VHM48_08710"
     CDS             4123..4410
                     /locus_tag="VHM48_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755531.1"
                     /translation="MTDHEAIDAGASAPPIEADADALERAESDAAAEAAAVAPADPAT
                     AGGADEIDDVLTSNDYTVAFSPGQVAVGLAIVAGLVAFAVRRRRRARREDG"
     gene            4414..5289
                     /locus_tag="VHM48_08715"
     CDS             4414..5289
                     /locus_tag="VHM48_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX2755532.1"
                     /translation="MPVEPPIETPASLADAYAVLAASTDDAPITPIAGGTDIMVRITG
                     EIGEPPARMVDLSRLAELRGITVDGDAVILGATTTYTEIRRSDACREHLPALVEAAAT
                     IGAAQIQNRGTLGGNIANASPAGDTLPVLLALDAAIVVGGARGERTIPADEFWVAYRR
                     TALAPDELILRIRIPVTGGREARFRKIGTRRAQAISKVVLALAWREGGTGPRRPWSDV
                     RLALGSVADRPIRARATEGVLEGSAPTPETADGAAEALAAEIHPIDDVRSTADYRRVV
                     ASRALHRMIRDAGGW"
     gene            5283..5750
                     /locus_tag="VHM48_08720"
     CDS             5283..5750
                     /locus_tag="VHM48_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HEX2755533.1"
                     /translation="MVTTTPVDAGHSAAAALPPSAFVDKASQPTDDAVERGLGVAAPF
                     WLALRSGIAVAFAPVDEGWTYSGRKHGWALRLRQRDRSILYLTPLAGGLRASLALPER
                     AVPVALDAGLPATIRAIVATAVSNSEGRAVRIDVRSDEDVAGVLALARIRMAS"
     gene            5793..7292
                     /locus_tag="VHM48_08725"
     CDS             5793..7292
                     /locus_tag="VHM48_08725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2755534.1"
                     /translation="MTTTDPLNGVRPGGRRSFWLRAALAAEPSSLAADVAAPPFTGRA
                     RADVVIVGGGYTGLWTAIRIRELEPSARVVVIEQDICGGGPSGRNGGFVTGWFDELPA
                     LTRQFGEAEALRTAEALDIEIASLGPWTAAHGIDAGLVEKGFVQVSAAPAQDDAWLEA
                     TEACARLGMGDRWRALTAADVAERARSPVFRGGAFMPGAGTVQPARLARGLRRVALEQ
                     GIVIHERTTATLPADPPRRAAGAASGPIDIATISAEGSGAIEADQVVVAANAWAAAWR
                     PVGRRLLNWSSYIVLTEPIPDRLAEIGWTGGEGIADARFTLHYARTTADGRIALGGGG
                     GRAGWGGRIDRAFTHDAGAARRAAEGLRRWFPSLADVRIEDAWGGPIDITDDHRAWFG
                     SRPGGRIHHGLGYSGNGVAPAILGGRILAALATGRGRDDVAASLPIVGERATPRAFPP
                     EPFRGAGARVFREAMVRREHAEEAGEAPSRVVHEISRIPRRLGYHLGPG"
     gene            7289..7705
                     /locus_tag="VHM48_08730"
     CDS             7289..7705
                     /locus_tag="VHM48_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2755535.1"
                     /translation="MSRVPVEHVNATAYRRAADAFRAGDLATIETLVDEDVVWHVPGG
                     HPRAGDIHGRAALIDWLAALAGTGFWLAEHDVFGNDEHVCALSIMGARRPDVEVETRV
                     VSIFHFRDGRQVERWFFPEDRDAWDRIFTDPPTMTR"
     gene            7702..9270
                     /gene="aceB"
                     /locus_tag="VHM48_08735"
     CDS             7702..9270
                     /gene="aceB"
                     /locus_tag="VHM48_08735"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX2755536.1"
                     /translation="MIEIAGSAVPGVERILTPDALAFLADLQATFGVRREALLQARLD
                     RQAALDGGADLDFDPATADLRAADWTVPPAPADLDDRRVEITGPAEPKMMINAFNSGA
                     RVFMADLEDALSPTWANVIGGQVALRDAVRGQLTFTSPEGKAYRVGERPAQLVVRPRG
                     WHLEEGHVTVDGRPMSASLVDAGLYLFHNGTERIRRGTAPYFYLPKLQSAAEAALWHD
                     VFARAEERLGVPPGSVRTTVLIETIHAAFQMDEILHALGPYAAGLNAGRWDYLFSCIK
                     TFRGRADRILPERAQLTMTTPFMRAYTELLVRTCHRRGAHAIGGMAAFIPSRRDPVVN
                     DAAMARVRDDKERESRDGFDGTWVAHPDLVPLATEIFDGVLGDRPHQKERGRDEVHVT
                     AAELLDFAVPGGRVTEAGVRLNVSVALQYLDAWLTGNGAAAINNLMEDAATAEISRSQ
                     LWQWRVGGAQMEDGRFVTGGLYTAIRDEELASLGGPAGGNGHLGDAAELLDGLVLDDT
                     FTEFLTIPAGALLD"
     gene            9786..10949
                     /locus_tag="VHM48_08740"
     CDS             9786..10949
                     /locus_tag="VHM48_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX2755537.1"
                     /translation="MALPTKATHQQTRVHNERLVVRTLYDLGPISRAEVARLTGLTRT
                     TVSDVVATLLGDGVVREIGRGPSSGGKAPILLQVDDDARLVVGLDLGEEHFAGSLVNL
                     RGEIRRSVELAVDGRDGDAALALVFELLDQLLDGSAAPLLGIGIGTPGLVDSRTGTIR
                     RAVNLDWRDLPLGEIVAERYDVPVNVANDSQAAALAEYTYAGGDRVPNLIAIRVGRGV
                     GAGLILRGALFQGDGSGAGEIGHVVVDADGALCRCGRTGCLETIAGMRAVEAQAAAAT
                     GQPTDLAAIRAAIDGGEPWAVAIADDAGSALGRAIAGLIGALDIQRIVLLGPVTDLGA
                     PWLAAVRREAGSRALALLADDVDIAVARPTTNVVIRGASALLVARELGLSLVR"
     gene            10939..>11126
                     /locus_tag="VHM48_08745"
     CDS             10939..>11126
                     /locus_tag="VHM48_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755538.1"
                     /translation="MSDDDADHHVAGRLRRLAEHDRIGSPLRSELGPGEPPERVEETL
                     RAIGLTAPGELVELFGWR"
BASE COUNT         1517 a         4158 c         3962 g         1489 t
ORIGIN      
        1 cgaggtcgag gtcatcggtg aggagggggg ctcgatccgg gttcgcacgc ccgatggcat
       61 cgagggatgg gtgccgcgcg tggtcatcgt cggatcacct gcgcgggaac gagccccgtc
      121 ggcatctcaa ccggagccgc ccgtggcccg gcgccgctgg agtttcccga cactgcgccg
      181 aggatcgacg acgaccgatc ccgccgcgga gtcaccgcgg ctctgatccg ggccggctcc
      241 gactcgggtc cacccagtct ggcctgctga cgtggcgaat cctcgaacgg gtgctcggtg
      301 ccccgaacgc gcgcctcagt ccggcgggcc ggcctcaagc tcggctgcaa gtcgggccag
      361 cgcgagatcg atcgcatcgg acaggtcgcc cttgtcgggc ggactcgccc atccgaagcc
      421 gatcgtcaca tcgtcgccga cggccttgag ctccggctcg cacgacgccc gcgctcgctc
      481 gacgaagttg atcgcggcga tctccgtggt ctcggtcagg aggatcccga accgcgcctg
      541 ctcgagccgg gcgatgtgat cgctggagcg gatctcgagg ccgagacgcc gggcacaccc
      601 ggcgagcgcc cgctcggcca cgtcccaacc ccattgcccg gccaggcggt cgaggccgac
      661 gatctcggcg aacgcgacgg tgacgggccg gtggtagcgc ccgacgcggg cctgctcgct
      721 caggacgatt cgatcccaga gctgtggtcc atcggcgccg gtgagcgggt cggaccagcc
      781 ctgtgggggt gcgatcgggc ggaccacgtg gggcacctcg aacgtggcga tcgagaccat
      841 ccaggtctgg cgggccacga gctggatgcg ctcggccgag gcggcgtcga ccaccggaca
      901 cgtcggatcg gcggcgcgtt cgtcgagcgt cggcgcgatc ggcgaggtcg cgctcgactc
      961 gccgtcgttc tcggacatgg ttgcgtccct cagtcgcgtc ccatcgctcc cgatggtcaa
     1021 cgggccgtcg cgacccccgg tccggcgtga tccggatctt ccgccagttc gatgatccag
     1081 acccctcccc attggtaccg gcgtggtcct gaccgcccgg tcgggcatag ccgggtgata
     1141 ctctcgcagg ccaaggaggg acgccacacc gcgatgacga tccggacctc gcccagccgg
     1201 accaccaccc cgatcgcccc acagcgcagc gcgccagacg cgctcgtcgt gccccgcggc
     1261 gccgtcgtcc ccggccgcgc gcgaccggcc gccccgcttc gccgggaggg ccccgcgaag
     1321 ctcaccggcg aagcgaagta cgcggacgat ctcgtctttc cgggcgcctg gtacggcgcc
     1381 acgatccgct cgaccgacgc ccacgcccgg ttcgacgggt tcgagctcga tcccacgctc
     1441 gactggtcga agatcgcggt cgtcaccgcc gaggacatcc ccggcgacaa catcgtgagc
     1501 ctgatcagcg acgaccagcc ggtcctcgtc gcggtcggtg gcgagatcca gcaccacgcc
     1561 gagccgctcc tgctcctggc cgcggcggat cgcgagatgc tccggcatgc ccgccacggc
     1621 gtgacgatcc gctccacgcc cctccccccg gtcttcgacc cgctcgagag cgaccacgtc
     1681 ttcgctcact acgagatcgc ctctggtgac ctcgagcagg gcttcgccgg ggcggactac
     1741 atcctcgagg gcgagtaccg cgtcggccac caggagcagc tctacatcga gaacaacgcg
     1801 atgatcgcgg tgccgcggga ggacggcggc gtcgcggtcc acggctcgct ccagtgcccg
     1861 tactacatcc acaaggcgct gaagcgcgcg ctcctcctga catccgagca ggcccaggtc
     1921 gtccaggcgg agaccggcgg cgggttcggt ggcaaggagg aatacccctc gatcgtcgcc
     1981 attcacgccg cccttcttgc gcgcaagagc ggcaagccgg tccggatgat ctacgaccgc
     2041 cacgaggacc tcgcggcgac cacgaaacgg caccccgcga tcgtcaccca tcggaccggc
     2101 gtcaaggccg atggaacgct cgtcgcccag gacatcgagg tcgtcatgga cgggggcgcc
     2161 tactgcacgc tgaccccggt ggtgctgagt cgtggcgcgc tccacgccgg cggtccctat
     2221 cgctgcgcca acgtccggat ccgggcgcgg gcaacccgga ccaacacgcc gcccaatggc
     2281 gcattccgcg gcttcggcgc gccgcaggtc gagttcgccg ccgagatcca gctcggccgt
     2341 gtcgccgagg ccctcgggat gagcccgctc gagatccgcc gccggaacct gtacaaggtc
     2401 ggcgacgaga ccccgaccgg gcaggtcctc cgcgagagcg tcgccgccga ggaggtcctc
     2461 gaacgggccg ccgaggcggc cgagttcgag ggggtcgccg cccggacgaa gaaggcccgc
     2521 gcccgccgag ccggctcggg tcacgtgccc tcgtcgccgc tccgcacgag ccgcgatcgg
     2581 accgcgtcgg ggatcggtgt cgcgctcgcc tggcacggtg ccgggttcac gggctccggc
     2641 gaggtcaagc tcgcgagcgt tgcgtccgtg gagctggccg ccgatgggcg ggtgcgggtc
     2701 ctcaccgggt cgaccgagat gggccagggg accaagacga tccttccgca gctcgtcgcc
     2761 gaggagctgg gactcgccgc ggccgacgtc gagatcgccc cgcaggacac cgcgatcgtg
     2821 ccggactccg gtccgacggt cgccagccgg accacgatgg tcgtcggcgg cctcgtgatc
     2881 caggcggcgc gacggctgcg cgcggacgtc gaggcgcgca acggcggcgc ggcgttcgcg
     2941 gatgtccggg tcgccgatgc cgccgcccac gggccgaccc gggtggacca gcagttcgag
     3001 ccgtacccgc acgtccactt cgatgacgag acgtaccgcg gcgacgcgta cccggcgttc
     3061 ggctgggccg cctgcgtcgc cacggtcgat gtcgatctcg acagcggcga ggtcacggtc
     3121 cgcgacgtcg tcgccgccga cgatgtcggc cgggtcatcc accccatcct cgccgcgggc
     3181 caggtcgagg gcggcacgct ccaggcgatc ggctacgcga cgatcgagga gatcaagctc
     3241 gacgacggtc ggtatctcaa cgaccgcctt gcgacgtaca tcatcccgac cgccctcgac
     3301 gcgccgcgga tccacgcgat cctcgtcgaa gcgccgttca gcggcgcgcc ccacggcgcc
     3361 aagggagtcg gcgagctgcc gatggacgtc ggggcgccgg ccgtggtggc cgcgatccac
     3421 gacgccaccg gggtctggat ccacgagctg cccgcgtcgc cggagcggat tctcgccgcc
     3481 ctcgggatga cgccgtcacc ggacgcgcct gcgccgtcgc cggccgcgac cgaggcggcc
     3541 gtcagcgacc cgatcgacgg gggcacggac ccccgcccgg acgcgccgac cgatccccag
     3601 ccgatgcgcg gcccgcgcga ccccggcgaa cgtcgatgag acgaccggca tgacgacctg
     3661 gcacttcacg ctcaacggtg acccggtcga gatcgaggcg cccggaatgc gacgcctgct
     3721 cgacgtcctc cgcgaagagc tcgccctgac ggggacgaag gagggctgcg gcgaaggcga
     3781 atgcggcgcc tgcaccgtcc tcctcgacgg cgcgcccgtc gactcgtgcc tcgttcccgt
     3841 ctgccaggtc gacggggccc ggatcgcgac cgtcgagggg ttggcggcgc gctcgcccga
     3901 gggcgcgctg ctcgacccgc ttcagcaggc gttcctagag gcgggcggcg cccagtgcgg
     3961 catctgcaca cccggcatgc tgatggccgc tcgtgcgtac ctggacgccg gcggcggccc
     4021 ggacgagggc gcgatccgga cagcgatcgc cggcaacctg tgccgctgca cgggctacac
     4081 caagatcgtc gacgccatcg cgatcgcagc cggaggccga agatgaccga tcacgaggcg
     4141 atcgacgcgg gcgcctccgc cccgccgatc gaggccgatg ctgacgcact cgagcgagcc
     4201 gagtccgacg cggccgccga ggcggcggcc gtcgccccgg ccgatcccgc gaccgcgggc
     4261 ggggccgacg agatcgacga cgtcctgaca agcaacgact acaccgtcgc cttcagcccg
     4321 ggccaggttg ccgtcggact cgcgatcgtc gccggtcttg tcgcgttcgc ggtccgccgc
     4381 cggcgccggg cacgccgcga ggacggctga accgtgcctg tcgagccgcc gatcgagacg
     4441 cccgcgtcgc tggctgacgc ctatgccgtc ctcgccgcct cgacggacga tgcgccgatc
     4501 accccgattg ccggcggcac cgacatcatg gtccggatca ccggcgagat cggcgagccg
     4561 cctgcccgga tggtcgacct ctcccggctc gccgagctcc gcgggatcac tgtcgatggc
     4621 gatgccgtca tccttggcgc cacgacgacc tacaccgaga tccgtcgatc cgacgcctgt
     4681 cgggagcacc tgcccgccct ggtcgaggcg gccgccacga tcggcgccgc ccagatccag
     4741 aaccgcggga cgctcggcgg caacatcgcc aatgcgtcgc cggccggcga taccctgccg
     4801 gtcctgctcg cgctcgatgc ggcgatcgtc gtcggcggcg cgcgcggcga gcggacgatc
     4861 ccggccgacg agttctgggt cgcctatcgg cggaccgccc tcgcccccga cgagctgatc
     4921 ctccgcatcc ggatccccgt caccggcggt cgggaggcgc gcttccgcaa gatcggcacg
     4981 cgtcgggcgc aggcgatcag caaggtcgtg ctcgccctgg cctggcgcga gggcggaacg
     5041 ggaccgcggc gcccctggtc cgacgtccgg ctggccctcg gctcggtcgc cgaccggccg
     5101 atccgggctc gggcgaccga aggggtcctc gaagggtcgg cgccgacgcc cgagaccgcc
     5161 gatggggcag ccgaggcgct cgcggccgag atccacccga tcgacgacgt ccgctcgacc
     5221 gccgactacc gccgggtcgt cgcctcccgg gcgctccacc ggatgatccg cgacgcgggc
     5281 ggatggtgac gaccacgccg gtcgatgccg ggcactcggc ggctgccgcg ctgccgccga
     5341 gcgcgttcgt cgacaaggcg agccagccga ccgacgacgc agtcgagcgc gggcttggcg
     5401 tggctgcccc gttctggctc gcgcttcgat ccgggattgc ggtcgcgttc gcgcccgtcg
     5461 acgagggttg gacctactcg ggccggaagc acggctgggc gctccggctc cggcaacgcg
     5521 atcggtcgat cctgtacctg accccgctcg cgggcggctt gcgggcaagc ctggccctgc
     5581 ccgagcgggc cgtgccggtc gcgctcgacg ccggcctgcc cgcgaccatc cgggcgatcg
     5641 tcgcgaccgc ggtgtcgaac tcggagggtc gggcggtccg gatcgatgtc cgctccgacg
     5701 aggacgtggc cggggtcctg gcccttgccc ggatccggat ggcgagctga ggctcgaacg
     5761 cagcccgtga cgagcatgcc gagccgccct tcatgaccac caccgatccc ctgaacgggg
     5821 tccggccggg cggccgacgg tcgttctggc tgcgcgcggc gctcgcggcc gaaccgtcgt
     5881 cgctcgccgc ggacgtcgcc gcgccgccgt tcaccggccg tgcccgcgcc gacgtcgtga
     5941 tcgtcggcgg cgggtacacc ggcctctgga cggcgatccg gatccgggag ctcgagccgt
     6001 cggcgagagt cgtcgtcatc gaacaggaca tctgcggcgg cgggccgtcc gggcggaacg
     6061 gcggcttcgt caccggctgg tttgacgagc tgccggcact gactcgtcag ttcggtgagg
     6121 cagaggcgct gcggacggcc gaggcgctcg acatcgaaat cgcatcgctc ggtccgtgga
     6181 cggcggccca cgggatcgac gcgggcctcg tcgagaaggg cttcgtccag gtcagcgccg
     6241 cgccggccca ggacgacgcc tggctcgagg ccaccgaggc gtgcgcccga ctcggcatgg
     6301 gcgatcgctg gcgggcgctc acggcagcgg acgtcgccga gcgagcccga tcgccggtct
     6361 tccgcggcgg cgccttcatg ccgggcgccg ggaccgtcca gccggcccgc ctcgcccggg
     6421 gcctccgccg cgtcgcgctc gagcagggga tcgtcatcca cgagcggacg accgccacgc
     6481 tccccgccga cccgccgcgg cgcgctgcgg gcgcggcgtc tggcccgatc gacattgcca
     6541 cgatttccgc cgagggctcc ggcgcgatcg aggccgatca ggtcgtcgtc gccgcgaacg
     6601 cctgggccgc cgcctggcga ccggtcgggc ggcgcctcct caactggtcg agctacatcg
     6661 tcctgaccga gccgatcccc gaccgtctcg ccgagatcgg ctggaccggg ggtgagggga
     6721 tcgccgacgc acgcttcacc ctccattacg cccggaccac ggccgacggc cggatcgcgc
     6781 tcggcggcgg tggcggtcgg gccggctggg gtggccggat cgatcgagcg ttcacccatg
     6841 acgccggcgc ggcgcggcgc gcggccgagg gcctccggcg ctggttcccg agcctcgccg
     6901 acgtccggat cgaggacgcc tggggcggcc cgatcgacat caccgacgac caccgggcgt
     6961 ggttcgggtc gcggcccggc gggcggatcc atcacgggct cggctacagc ggcaacggcg
     7021 tggcgccggc gatccttggc ggccggatcc tggcggctct ggcgacgggc cgggggaggg
     7081 acgacgttgc ggcgagcctg ccgatcgtcg gcgagcgggc gacgccgcgg gcatttccgc
     7141 cggaaccgtt ccggggcgcc ggggcgcggg tgttccgcga ggcgatggtc cggcgagagc
     7201 acgcggagga ggcgggtgag gcaccttcgc gggtcgtgca tgagatttcg cgcatcccgc
     7261 gacgcctcgg ctaccacctc ggccccggat gagccgcgtg cctgtggagc acgtgaatgc
     7321 gacggcctac cgccgcgctg ctgacgcgtt ccgcgccggc gacctcgcga cgatcgaaac
     7381 gctcgtcgac gaggacgtcg tctggcatgt cccgggcggc cacccgcggg ccggcgacat
     7441 ccacgggcgt gctgcgctga tcgactggct cgcggcgctc gccgggaccg gcttctggct
     7501 ggccgagcac gacgtgttcg gaaacgacga gcacgtctgc gcgctgagca tcatgggcgc
     7561 gcgccggccc gacgtcgaag tcgagaccag ggtcgtcagc atcttccatt tccgcgacgg
     7621 ccgccaggtc gagcgctggt tcttcccgga ggaccgcgac gcgtgggacc ggatcttcac
     7681 ggatccgcct acgatgaccc gatgatcgag atcgccgggt ccgccgttcc cggcgtcgag
     7741 cggatcctga cgcccgacgc cctcgccttt ctcgccgacc tccaggcgac gttcggggtg
     7801 cggcgcgagg cgctcctcca ggctcgtctc gatcgccagg ccgcgctcga cggcggcgcc
     7861 gacctcgact tcgaccccgc gacggccgat ctccgtgccg ccgactggac ggttccgccg
     7921 gccccggccg acctcgacga ccggcgggtc gagatcacgg gcccggccga gccgaagatg
     7981 atgatcaacg ccttcaactc gggcgcccgg gtgttcatgg ccgacctcga ggacgccctc
     8041 agtcccacct gggcaaacgt gatcggcggc caggtcgccc tccgcgacgc cgttcgcggc
     8101 cagctcacct tcacgagccc cgaaggcaag gcctaccggg tgggggagcg ccccgctcag
     8161 ctcgtcgtcc ggccgcgcgg ctggcacctc gaggagggcc acgtcaccgt cgacgggcgc
     8221 ccgatgagcg cgtcgctcgt cgacgccggg ctctacctgt tccacaacgg cacggagagg
     8281 atccgccgtg ggacggcgcc gtacttctac ctgccgaagc tccagtccgc cgccgaggcc
     8341 gccctctggc acgacgtctt cgcccgggcg gaagagcgcc tcggggtccc gcccggttca
     8401 gtgcgcacga ccgtcctgat cgagacgatc cacgcggcgt tccagatgga tgagatcctc
     8461 catgctctcg gaccgtatgc cgcgggcctg aacgccggcc gctgggacta cctgttcagc
     8521 tgcatcaaga cgttccgcgg ccgggccgat cggatcctgc cggagcgggc ccagttgacg
     8581 atgacgacgc cgttcatgcg ggcgtacacg gagctgctcg tccggacgtg ccaccgccgc
     8641 ggcgcccacg cgatcggcgg catggcggcc ttcatcccgt cgcgccgcga tccggtcgtc
     8701 aacgacgcgg cgatggccag ggtccgcgac gacaaggagc gcgaatcgcg ggacggcttc
     8761 gacgggacgt gggtcgccca cccggacctc gtgccgctcg cgacggagat cttcgacggc
     8821 gtcctcggcg accggccgca ccagaaggag cgcggccgcg acgaggtcca cgtcacggcg
     8881 gccgagctgc tcgacttcgc ggttccgggt gggcgggtca cagaggccgg ggtccggctc
     8941 aacgtgtcag tggcgctcca gtacctcgac gcctggctga ccggcaacgg cgcagcggcg
     9001 atcaacaacc tgatggagga tgccgcgacg gccgagatct cccgctccca gctctggcag
     9061 tggcgggtcg gcggggcaca gatggaggac ggccggttcg tgaccggcgg cctctacacc
     9121 gccatccgcg acgaggagct ggcgtcgctc ggcggcccgg ccggcggcaa cggccacctc
     9181 ggcgacgccg ccgagctcct cgacggcctc gtcctcgacg acacattcac cgagttcctg
     9241 acgatcccgg ccggtgccct gctcgactga cgacccgacg acgatgcccg gccgctcgtc
     9301 cgccgacctt ctcgcgatgg ccgatcgcaa gtccgaggcc tttcttcggg ccgccgcgac
     9361 ccggccgtgg gcgacggtcc acctgggcga cgatgtgatc cacggcacga gcgacatccc
     9421 gctgctcggc gcgaacgcgg gacgcccgag gcccacgcgc gttggcggcg gcggccgatt
     9481 gtgtatgaga ggagcaactg gaccgcggac cggcgcggaa gcggcctgcc ggacgcgatt
     9541 catgtccgat ctgctcctca ggtggacaca tcggacgcgg ttggatcgtt caccggcgaa
     9601 ttccgggatg tcggcggtcc agttgctcgc cacgcgcaca gcggacggga acggcccgcc
     9661 acccgcccgg cgatctttgt ccaccgactt gacaaaggcc gcccggaggt ggcactttgt
     9721 ccatagactg gacgaagtcg cgcggaggag cgcggcggcc ggtcgacgga ggcccgaccc
     9781 ggccgatggc gctcccgacc aaggcgaccc atcagcagac ccgcgtccac aacgagcggc
     9841 tcgtcgtccg cacgctgtac gacctgggac cgatcagccg cgccgaggtc gcccgtctca
     9901 ccggcctgac ccggacgacc gtctcggacg tcgtcgcgac gctcctcggg gacggcgtcg
     9961 tccgcgaaat cgggcgcggg ccctcgagtg gcggcaaggc cccgatcctc ctccaggtcg
    10021 acgacgatgc ccgcctcgtc gtcgggctcg acctcggcga ggagcacttc gcgggctcgc
    10081 tcgtcaacct ccgcggcgag atccgccggt cggtcgagct tgcggtcgac ggtcgcgacg
    10141 gcgacgccgc cctggccctc gtcttcgagc tcctcgacca gctcctcgat ggcagcgccg
    10201 cgccgctgct ggggatcggg atcgggacgc ccggcctggt cgacagccgg accggcacga
    10261 tccgccgcgc cgtcaacctc gactggcgcg acctgccgct cggcgagatc gtcgccgagc
    10321 ggtacgacgt cccggtcaac gtcgccaacg attcccaggc ggcagccctc gccgagtaca
    10381 cgtatgccgg cggggatcgg gttccgaacc tgatcgcgat ccgcgtcggg cgcggcgtag
    10441 gcgccggcct gatccttcgc ggcgcgctgt tccagggcga cggctccggc gccggcgaga
    10501 tcggccatgt cgtcgtcgac gccgacggcg cgctctgtcg ctgcggccgg accggctgtc
    10561 tcgaaacgat cgccggcatg cgcgccgtcg aggcacaggc cgcggcggcc accggacagc
    10621 cgaccgacct cgcggccatc cgggccgcga tcgacggcgg cgagccgtgg gccgtcgcca
    10681 tcgccgacga cgccgggtcg gctctcggcc gggccatcgc cggcctgatc ggcgccctcg
    10741 acatccagcg gatcgtcctg ctcggcccgg tcaccgatct cggcgcgccg tggctggcag
    10801 ccgtccgtcg cgaggccggc agccgggccc tcgccctgct cgccgacgac gtcgacattg
    10861 ccgtcgcccg accgacgacc aacgtcgtca tccgtggagc gtcggcactg ctggtcgccc
    10921 gcgagctcgg cctgagcctt gtccgatgac gacgccgatc accacgtcgc cggccggctc
    10981 cgacgcctgg cggagcacga tcggatcgga tcgccgctcc ggagcgagct cggacccggt
    11041 gaaccgcccg agcgcgtcga ggagaccctc cgcgcaatcg gtctcacggc gccgggcgag
    11101 ctcgtcgagc tgttcgggtg gcgcca
//
LOCUS       DATAIW010000277         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_76993, whole genome shotgun sequence.
ACCESSION   DATAIW010000277 DATAIW010000000
VERSION     DATAIW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76993"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VHM48_08750"
     CDS             <1..1570
                     /locus_tag="VHM48_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX2755539.1"
                     /translation="GYGKTEVALRAAFKAIQDGKQVAVLVPTTVLAAQHHQTFSQRFA
                     AFPMKVRLLSRFVSVREQATTIDGLADGSVDLVIGTHRLLSKDVHFRDLGLVVVDEEQ
                     RFGVAAKEKLKQLRREVDVLTLSATPIPRTLNLALAGVRDMSVMETPPEDRLPIQTRV
                     AEASAGLVRDAILRELDRGGQVFFVHNRVETIEAQTEQLRRMLPGARFVVGHGQMAEG
                     HLESVMITFAAGEADVLVCTTIIESGLDIPNANTIVIDRADTLGLAQLYQLRGRVGRS
                     SRRAYAYLLYRRRERMSEEARKRLQAIFNASELGAGFQIALADLEIRGAGNILGGEQS
                     GHMAAVGFDLYSRLLADAVEERKALREDRPVRVERPQAVVDLPVEAHLPDDYVPEEAQ
                     KLELYRRLARSRTAGDLAAFRQELTDRFGPMPPPVVRLVEVAELRLTAEAAGVASMSR
                     EDGQLVVRFGEGMSRATAMRLLAGGGLPGVRPSEVTFASNQVRIRLPRDGSRAWGLTQ
                     AVVARLSNDGGAGS"
     gene            1703..2065
                     /locus_tag="VHM48_08755"
     CDS             1703..2065
                     /locus_tag="VHM48_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755540.1"
                     /translation="MTTAILVAGCGSTVPEPTIIAIPTSAAGGGCLDAPVGGRVITSQ
                     RWGIAIADETGRVSKLFWPNGVHGQMDGDRVALVDGSGRLVAHVGDTIRSGGGFIGAN
                     GDPDNTMMVCGEIRAIVP"
     gene            2062..2349
                     /locus_tag="VHM48_08760"
     CDS             2062..2349
                     /locus_tag="VHM48_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755541.1"
                     /translation="MTRPDRLTSDDPVHELLLRIRVAGPEELRALRGALLAARATELS
                     EVQRRAQRHNLGYGTDSQRESMTDEVADRRRRWEMLDRLLEALESATRDHR"
     gene            2415..3284
                     /locus_tag="VHM48_08765"
     CDS             2415..3284
                     /locus_tag="VHM48_08765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2755542.1"
                     /translation="MARAVGKGARVVGGRRLTGGITSSIHRLTVETRTGVRQQVVLRR
                     WTPGATDDTADAPLFVERERHVLGRLEATDVPAPRFLAADPIGESTGVPALLMSRMSG
                     RMDLAPVDQKAWLRQIAVMAVRIHELDVDVQPYRWEARAVPVPAWASKPADWRAAAEL
                     VRGPAPDHDIRFSHGDYQHFNFLWQRGRLTGVVDWVSACRGPADVDVGHCRLNLAVLY
                     SAELAADFLAAYEAEAGRRVDPYWDIRCATAPAFEDWTQFIPIQVDGRAPFDRAGMTT
                     RVDELLSAALRRL"
BASE COUNT          503 a         1215 c         1211 g          533 t
ORIGIN      
        1 cggctacggc aagaccgagg tcgccctgcg ggccgcgttc aaggcgatcc aggacggcaa
       61 gcaggtcgcg gtcctcgtcc cgacgacggt cctcgccgcg cagcaccacc agacgttcag
      121 ccagcggttc gccgcgtttc caatgaaggt ccggctcctg tcgcggttcg tgtcggtccg
      181 cgagcaggcg acgacgatcg acggcctcgc cgacggctcg gtcgacctcg tgatcgggac
      241 ccaccgcctc ctgagcaagg acgtccactt ccgcgatctc ggcctcgtcg tcgtcgacga
      301 ggagcagcgc ttcggggtcg cggccaagga gaagctcaag cagctccgtc gcgaggtcga
      361 cgtcctgacc ctgtcggcga ccccgatccc gcggacgctc aacctcgccc tcgcgggcgt
      421 ccgcgacatg tcggtcatgg agaccccgcc ggaggatcgc ctgccgatcc agacgcgggt
      481 tgccgaggcg tcggccgggc tcgtccgcga cgcgatcctg cgcgagctcg accgcggcgg
      541 ccaggtcttc ttcgtccaca accgggtcga aacgatcgag gcccagaccg agcagctccg
      601 gcggatgctg ccgggcgccc ggttcgtggt cggccacggc cagatggcgg agggtcatct
      661 cgagagcgtg atgatcacgt tcgccgccgg cgaggccgac gtcctcgtct gcacgacgat
      721 catcgagtcg ggcctcgaca tcccgaacgc gaacacaatc gtcatcgacc gggcggacac
      781 cctcgggctc gcccagctct accagctccg cggtcgcgtc gggcggtcgt cgcgccgggc
      841 ctacgcctac ctgctgtacc gccggcgcga gcggatgagc gaggaggcgc gcaagcggct
      901 ccaggcgatc ttcaacgcct cggagctggg cgcagggttc cagatcgccc tcgccgacct
      961 cgagatccgg ggagcgggca acatcctcgg cggggagcaa tcggggcaca tggccgcggt
     1021 cgggttcgac ctctactcgc ggctcctcgc cgacgccgtc gaggagcgca aggccctccg
     1081 cgaggaccgg cccgtccgcg tcgagcggcc gcaggccgtc gtcgacctcc cggtcgaggc
     1141 gcacctgccc gacgactacg tgcccgagga ggcccagaag ctcgagctgt accgccgcct
     1201 cgcccgatcc cgtaccgctg gcgacctcgc cgcgttccgc caggagctga ccgaccgctt
     1261 cgggccgatg ccgccgcccg tcgtccgcct cgtcgaggtc gccgagctgc ggctcaccgc
     1321 cgaggccgcc ggggtcgcct cgatgtcgcg cgaggacggc cagctcgtcg tccggttcgg
     1381 cgaggggatg tcgcgagcga cggcgatgcg cctgctcgcc ggcggcggtt tgccgggggt
     1441 gcgtccgtcg gaggtcacgt tcgcgagcaa ccaggtccgg atccgcctgc cgcgcgacgg
     1501 gtcgcgggcg tggggcctga cccaggccgt ggtcgcgagg ctgtccaacg acggcggcgc
     1561 cggatcgtga gcgaacgagg gattctgatc ggctgtctcg tgctggtgat cctcgtcctc
     1621 gtcgtgacct ggatcattcg atccggcctc agctcgatgt cgtactgacg tgcgggtcca
     1681 ccgcaggatc gccctggccg tgatgacgac cgcgatcctc gtggccgggt gcggctcgac
     1741 cgtgcctgaa ccgacgatca tcgccatccc gacgagcgcc gccggcgggg ggtgcctgga
     1801 tgcgccggtc gggggacggg tcatcacgtc acagcgctgg gggatcgcga tcgccgacga
     1861 gacggggcgc gtctcaaagc tgttctggcc aaacggcgtt cacgggcaga tggatggtga
     1921 tcgcgtcgcc ctggtcgacg gatccggccg tctcgtggcc catgtcggtg acacgatccg
     1981 ctcgggcggc gggttcatcg gcgcaaacgg cgacccggac aacacgatga tggtctgcgg
     2041 cgagatccgg gcgatcgtcc cgtgacccgg cccgacaggc tcacgtccga tgaccccgtt
     2101 cacgagctcc tcctccggat ccgggtggcc ggcccggagg agctgcgggc cctgcgcggt
     2161 gccctcctcg cggcccgcgc gaccgagctg agcgaggtcc aacgtcgtgc gcagcgccac
     2221 aacctcggct atgggaccga ctcccagcgc gaatcgatga ccgacgaggt cgccgaccgc
     2281 cgacgccgtt gggagatgct cgaccgattg ctcgaagcgc tggaatcagc aacccgcgac
     2341 cacaggtgac agcacgcatt gcgacggcgc atacgagaca ccaggtaaag cttgatccgc
     2401 gcacacgcga gtgggtggcg cgcgccgtcg gcaaaggcgc acgagtcgtc ggggggcgcc
     2461 gcctcaccgg cggcatcaca tcgtcgattc atcggttgac cgtcgagacg cggaccggtg
     2521 tccgacagca ggtagtcctg cggcgatgga cgcctggcgc gacggacgac acagccgacg
     2581 cgccgctgtt cgtcgagcgc gaacgccacg tgctcggccg ccttgaggcg accgacgttc
     2641 ccgctccgcg attcctcgcc gccgacccga tcggcgagtc cacgggtgtg ccggcgctgc
     2701 tgatgagccg catgtccgga cgaatggatc tggcacccgt tgaccagaag gcttggctgc
     2761 ggcagatcgc ggtgatggcg gttcgcatcc acgagttgga cgtcgacgtc cagccgtatc
     2821 ggtgggaggc gcgggccgtg cccgttccgg catgggcgag caagccggcc gactggcgcg
     2881 ccgctgccga gctggtccgc ggacccgcgc ccgaccacga catccgcttc tcgcacggtg
     2941 actaccagca cttcaacttc ctgtggcagc gagggcgcct taccggtgtc gtcgactggg
     3001 tctccgcgtg ccgcgggccg gccgacgtcg atgtcggcca ctgccggctc aatctggcag
     3061 tcctctactc ggccgagctg gccgccgatt ttctggcggc ctacgaggcc gaagcgggcc
     3121 ggcgcgtgga tccgtactgg gacatccggt gcgcgaccgc gcccgctttc gaagactgga
     3181 cccagttcat cccgatccag gttgatggcc gtgcgccgtt cgatcgggcc ggtatgacga
     3241 cgcgggtcga cgagttgctc agcgccgcgc tacggcggct ttagcagcgc ggcccacgtg
     3301 acatttgccg acgcttagcg accgcggcgt gagctagctc gggatggttt ttctcacgtc
     3361 cgagggcaat acagggtctt catcggttgc caagacccgc ccgtcacgta accgtagatc
     3421 catgattgct cggtcccggc gagtgggacc ctcgttgatg gg
//
LOCUS       DATAIW010000278         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_47117, whole genome shotgun sequence.
ACCESSION   DATAIW010000278 DATAIW010000000
VERSION     DATAIW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47117"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..801
                     /locus_tag="VHM48_08770"
     CDS             34..801
                     /locus_tag="VHM48_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2755543.1"
                     /translation="MDLGLGGLRALVGGGSSGLGAAIATVLRAEGSAVSLAARPSDRL
                     DEQARRIDAIAIPTDLSSVDGPAAAVAATVERLGGLDLLVVNSGGPPGGDFAALDDAT
                     WTVAVDGVLHSAIRLIRAGLPHLRASPSAAILIILSSSVREPIPGLTTSNVLRPGLVG
                     LIKSLTAEIAPIRINGIAPGRVATARIAHLDGLRATSTGRPIEDIEREQIGRIPLGRY
                     GEPAEVGRLCAFLLSPAASYVSGAIVPVDGGMIRALP"
     gene            complement(861..1280)
                     /locus_tag="VHM48_08775"
     CDS             complement(861..1280)
                     /locus_tag="VHM48_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755544.1"
                     /translation="MHRRPLGAGRRLAGLAAIVIIAACFLPWWQSSAEFGLTPLSNNA
                     FDGAGVLVFFAALAVIALLALPYAAGDVPVGIDRPLSFVIVTVVAWLAMGIHAVGIAT
                     RNLEALLPQRAYGLWLAVLGLVLLSRAVYDITRERRH"
     gene            complement(1283..1852)
                     /gene="hpt"
                     /locus_tag="VHM48_08780"
     CDS             complement(1283..1852)
                     /gene="hpt"
                     /locus_tag="VHM48_08780"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2755545.1"
                     /translation="MTEARIDLVADIGEVLLGEEQIAAKVAELGARIGADYGDRRLTL
                     VSVLKGSLPFMADLMRAIPSPVRIDLMEVSSYGGTSTESSGLVRILKDLSATIEGEDV
                     LIVEDIIDTGLTLNYLIRYLRGKNPKSLRICTLLDKPARRLVDIPVDYVGFTIPDQFV
                     VGYGLDYGEYYRNLRFVGVLRPEVYTSPD"
     gene            complement(1849..2502)
                     /locus_tag="VHM48_08785"
     CDS             complement(1849..2502)
                     /locus_tag="VHM48_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755546.1"
                     /translation="MTASPATGSNSGRRGRRRSTAARPGTGPTWTSPGALGSIAPQVL
                     RPGPLSLDDVAPPDRPTPAFAHASEAEMARILDFYRIRWEYEPHTFPILWNLDGTVVE
                     SFSPDFWLPDLELYLEMTTLRQKLVRKKNRKLRRIRELYPDIRVKLFYARDFRALMLK
                     FGRLALADALSGTSGQVVPERNTAAIAMLASPDDAIDRDLDGPSLDDPSTIQPGGRV"
     gene            complement(2499..2891)
                     /locus_tag="VHM48_08790"
     CDS             complement(2499..2891)
                     /locus_tag="VHM48_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755547.1"
                     /translation="MDPRDDLDSLADGVDCAACGQFVPTDRIRVLARRDDIAFVEIHC
                     PGCSSESLGIVIASEDDDSTRDSSDATIAVGPGHVYGEFGPRDLERFREAVPIGSADL
                     DLVHDLLARGGLAALVGDTEPPAAGSGR"
     gene            3005..3376
                     /locus_tag="VHM48_08795"
     CDS             3005..3376
                     /locus_tag="VHM48_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2755548.1"
                     /translation="MIGVQPVPTSTTRRTPAPPPANFRHSYSKDKAGLIRRLARMEGQ
                     VRGIARMIEREEYCVDILQQTSALRAAVDALSILVLEDHVQGCVRTAAEHGDADAYVD
                     EVIDVVRRTLGRPVRSASRSS"
     gene            3602..5233
                     /locus_tag="VHM48_08800"
     CDS             3602..5233
                     /locus_tag="VHM48_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2755549.1"
                     /translation="MIDQRPDRLPAQPPNVSAPPAFSDRRVAHRRDEDQIAHREKVLL
                     ARALDVLASDSSAEERLAGLLRLLARTVGARRAAVVADGIERRAAVAVDADEDPATAE
                     ALAAWLDVHAPRTRARRAASGRAPISFIVAAGPSSGDGPDTTGADAADAVAGSTAFGN
                     STRGRLDEDGVPDRIAPPITPHYAMLPIPSAGEVALGFEFGRPVDADRLADRLPPTLA
                     RHAAVALALVTSQLATERELANLRARDAERTTFVSTVAHELRTPLTGLRGYLELILAG
                     QVGDPDVERDFLERSRSIVGSMGELVGDLLELSRLESGTLGLEIEPFSLAEACSQVAA
                     GLLPIAIERGIHLGTALPPRLRMARGDRRRVEQILTNLAANALKFTPPGGSVEIEARF
                     DGPVAIIAVRDDGPGIPDDDRERIFERFHRIAGHERITGTGLGLSIARDLSRRMDGDL
                     DVASVPGTGSTFVLALPGPTDVAASAVASALSEALEREQQGLEELLVRRAIDAGRIRA
                     ARSGGDDPTEHAVRRSGDTRLRALPTLRSETPSPA"
     gene            5720..6394
                     /gene="rsmG"
                     /locus_tag="VHM48_08805"
     CDS             5720..6394
                     /gene="rsmG"
                     /locus_tag="VHM48_08805"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX2755550.1"
                     /translation="MLLVWNEAVNLTAIREPAAIAIRHVVDSLTGLAVLHRRGVDRFI
                     DLGSGGGFPGITLAAALPADRALLIDSIGKKVRFLATVIDAIGLGDHAAAEAVRSESL
                     AASRADRGRWPAVTARAVGPLAELAELALPLLCPGGVLVAWKRVGVDDAADRRELEAA
                     RRALDAIDREARIDVVVAVDAMTARDAPGLGELVDHRLIVVERGPGSIDPTWPREPAT
                     RRRSPC"
     gene            6424..>7115
                     /locus_tag="VHM48_08810"
     CDS             6424..>7115
                     /locus_tag="VHM48_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2755551.1"
                     /translation="MRIAVLSDIHANVVALDSVLAAIGSVDAVWQLGDVVGYGPEPDA
                     VVDRLAEIGAVGVAGNHDLAALGGTEIDWFNPDAKAAMLWTRGRMGGRTRAWLAALPT
                     TRVEGGMTLVHGSPRDPVWEYITSMPVARANLTVLETTIGLHGHTHLPMAWADRDGRI
                     DAISPGPGSSFRLDGRPALLNPGSVGQPRDGDPSASWLEIDTDAEVATWRRVGYDIEA
                     VRTAMLAAGLPGR"
BASE COUNT         1023 a         2527 c         2569 g          996 t
ORIGIN      
        1 acgctgccgg caggcagccc gtaggatgca ccgatggatc tcggcctggg cggtctgcgg
       61 gcccttgtcg gcggtggctc gagcggcctc ggcgccgcga tcgcgaccgt cctccgcgcc
      121 gagggcagcg cggtctccct cgctgcccga ccgagcgatc gtctcgatga acaggcccgc
      181 cgcatcgacg cgatcgcgat cccgaccgac ctctccagcg tcgacggccc cgcggccgcg
      241 gtcgccgcga ccgtcgagcg gctcggtgga ctcgatctcc tcgtcgtcaa ctccggtggg
      301 ccgcccggcg gcgacttcgc ggcgctcgac gacgcgacgt ggacggtcgc ggtcgacggt
      361 gtcctgcatt ccgcgattcg cctgatccgg gcgggattgc cgcacctccg ggcgtcgccc
      421 tcagcggcga tcctgatcat cctctcgagc tcggtccgtg agccgatccc cgggctcacg
      481 acctcgaacg tcctgcgacc cggcctcgtc ggcctgatca agtcgctcac cgcggagatc
      541 gccccgatcc ggatcaacgg gatcgcaccc ggccgtgtcg cgacggcccg gatcgcccat
      601 ctcgacggcc tccgggcgac ctcgacggga cgcccgatcg aggacatcga gcgcgagcag
      661 atcggccgga tcccgctcgg tcgatacggt gaaccggccg aggtcggacg gctctgcgcg
      721 ttcctgctgt cgccggcggc gagctacgtc agcggggcga tcgtgccggt cgacggcggg
      781 atgatccgcg ccctgccctg aggggagcgc ggcgacgctc gcgagccagc cgcctcgctg
      841 gtggtggcgg cgtggcggcc tcagtggcgg cgttcgcggg tgatgtcgta gacggcacga
      901 ctgagcagga cgaggccgag cacggcgagc cacagcccat acgcccgctg cggcaggagc
      961 gcctcgaggt tcctcgtcgc gatgccgacc gcgtggatcc ccatcgccag ccaggcgacg
     1021 accgtcacga tgacgaagct caggggccgg tcgatgccga ccgggacgtc gcccgcggca
     1081 tagggcaacg cgagcagggc gatcaccgca agggcggcga agaagacgag cacgccggcg
     1141 ccgtcgaacg cgttgttcga caacggcgtc aggccgaact cggcagagct ctgccaccac
     1201 ggcaggaagc aggccgcgat gatgacgatc gcggccaggc cggccaggcg tcgtccggcg
     1261 ccgaggggtc gacgatgcat cgtcagtcgg ggctcgtata cacctcgggg cgcaggacgc
     1321 ccacgaagcg gaggttgcgg tagtactcgc cgtagtcgag gccgtagccg acgacgaact
     1381 ggtcggggat cgtgaagccg acatagtcga ccgggatgtc gacgaggcgc cgggcgggct
     1441 tgtcgaggag ggtgcagatc cggagcgact tcgggttctt gccgcggaga tagcggatca
     1501 gatagttcag ggtcaggccc gtgtcgatga tgtcctcgac gatcaggacg tcctctccct
     1561 cgatcgtcgc cgagaggtcc ttcaggatcc ggacgaggcc ggacgactcg gtcgaggtgc
     1621 cgccgtagga ggagacctcc atgaggtcga tccggaccgg ggacgggatc gcccgcatca
     1681 ggtcggccat gaacggcaat gagcccttga gcaccgagac gagggtcagc cggcggtcgc
     1741 cgtagtcggc gccgatccgg gcgccgagct cggccacctt ggcggcgatc tgctcctcgc
     1801 cgaggaggac ctcgccgatg tcggcgacga gatcgatacg ggcctcggtc acacacggcc
     1861 tccaggctgg atggtcgacg gatcgtcgag cgacggaccg tcgagatcgc ggtcgatggc
     1921 gtcgtcaggt gacgcgagca tggcgatggc cgccgtgttg cgctccggga cgacctgtcc
     1981 ggacgtgccg ctcagggcgt cggccagggc cagccgcccg aacttgagca tcagcgcccg
     2041 gaagtcgcga gcgtagaaca gcttgacccg gatgtccgga tagagctcgc ggatccggcg
     2101 cagcttgcgg ttcttcttgc ggacgagctt ctggcggagg gtcgtcatct cgaggtacag
     2161 ctcgaggtcc ggcagccaga agtcgggcga gaagctctcg acgaccgtcc cgtcgaggtt
     2221 ccacaggatc gggaacgtgt gcggctcgta ctcccagcgg atccggtaga agtcgaggat
     2281 ccgggccatc tcggcctcgc tcgcgtgtgc gaacgcgggt gtcggtcggt ccggcggcgc
     2341 cacatcgtcg agggagagcg ggcccgggcg gagcacctgg ggggcgatcg atcccagtgc
     2401 gcccggcgac gtccaggtgg gaccggtccc gggtcgggcg gcggtcgagc ggcggcggcc
     2461 gcggcgaccg gagttggacc cggtcgccgg gctggccgtc accggccgga acctgcggcg
     2521 ggtggctcgg tatcgccgac gagggcggcg agtccgcccc gggcgaggag atcgtggacg
     2581 aggtcgaggt ctgcggaccc gatcggcacg gcctcgcgga accgctccag gtcccgtggt
     2641 ccgaactcgc catagacgtg accgggcccg acggcaatcg tcgcgtcgga ggaatcgcga
     2701 gtggagtcat cgtcctcgga cgcgatcacg atcccgagcg actcgctgct gcagcccggg
     2761 caatggatct cgacgaaggc gatgtcgtca cggcgggcga ggacgcggat ccggtcggtc
     2821 ggcacgaact ggccgcaggc agcacagtcg acgccgtcgg cgagcgaatc gaggtcgtcg
     2881 cgcggatcca tcgaccgcaa gtctagccgg tcggtggcgc gaccgcgatc gagccgaccg
     2941 cggctcatcg atacccccac gttgcccgag gataccccca gggggtatca tggtcggacc
     3001 tcgcatgatc ggagtccagc ccgtgccgac cagcacgacc cgccgcacac ccgccccgcc
     3061 gccggcgaac ttccgccaca gctattccaa ggacaaggcc ggcctgatcc gacgccttgc
     3121 ccggatggaa ggccaggtcc gcggcattgc ccggatgatc gagcgcgagg agtactgcgt
     3181 cgacatcctg caacagacct ccgccttgcg ggcggccgtc gacgcgctct cgatcctcgt
     3241 cctcgaagac cacgtccagg gctgcgtccg gacggcggcc gagcacggcg atgcggatgc
     3301 ctacgtggac gaggtcatcg acgtcgtccg gcgtaccctc ggccggccgg tccggagcgc
     3361 cagccgctcc agctgacagg ccccaggcgc tcggcgccgc ggccgacaag cccggaggct
     3421 ccttccggcg tggcgcgcgt catcgcctcc gcctccgttc gccacatccc acccggtact
     3481 tccgggcggg atccatcggc ggccgggtga tgtccagacc accgatcggc gcgcgacgat
     3541 gggcgatgcc gctccacccg gttcggctcg ccatcgcgac gatgatccac gccaggggcc
     3601 gatgatcgac cagcgcccgg accgcctgcc ggcccagcca ccgaacgtct ccgcacctcc
     3661 cgccttcagc gaccgtcgcg tggctcaccg tcgggacgag gaccagatcg cccaccggga
     3721 gaaggtgctc ctggcccgcg ccctcgatgt cctggcatcc gacagcagtg ccgaggagcg
     3781 tctcgccggc ctgctccgcc tcctggcccg gaccgtcggc gcgcggcgcg cggcggtcgt
     3841 cgccgatggg atcgagcgac gcgcggcggt tgccgtcgat gcggacgagg acccagccac
     3901 ggccgaggcc ctcgccgcgt ggctcgacgt ccatgcgccg cggacgcgag ctcggcgcgc
     3961 ggcctcgggt cgggcaccca tctcgttcat cgtcgcggcc ggcccgagct ccggggacgg
     4021 gccggacacg accggcgcgg acgcggccga cgccgtcgcc gggtcgaccg cgttcggcaa
     4081 ctcgacccgt ggccggctcg acgaggacgg tgtcccggac cgcatcgccc ctccgatcac
     4141 cccgcattac gcgatgctgc cgatcccgag cgcgggtgag gtcgcccttg gcttcgaatt
     4201 cggacggccc gtcgacgccg atcggctggc cgaccggctc ccgccgactc ttgcccgaca
     4261 tgctgcggtc gcgctggccc tcgtcacgag ccagctcgcc acggaacgcg agcttgcgaa
     4321 cctgcgcgcc cgcgacgcgg aacggacgac cttcgtttcg acggtcgccc acgagctgcg
     4381 gacccccctg accggcctgc gcggctatct cgagctgatc cttgccggcc aggtcggcga
     4441 tccggacgtc gagcgcgact tcctcgagcg gagccgctcg atcgtcggct cgatgggcga
     4501 gctcgtcggc gatctcctgg aactgtcgcg gctcgagtcg gggacgctcg ggctcgagat
     4561 cgagcccttc tcgctcgcgg aggcgtgcag ccaggtcgcc gccggactcc tgccgatcgc
     4621 gatcgagcga ggcattcacc tggggacggc actgccgcct cgactgcgca tggctcgcgg
     4681 cgatcgccgc cgggtcgagc agatcctgac caacctcgcc gccaatgccc tgaagttcac
     4741 gccgcccggc ggcagcgtcg agatcgaggc gcggttcgac gggccggtcg cgatcatcgc
     4801 cgtgcgcgac gacgggcctg ggattccgga cgacgaccgc gagcggatct tcgagcgttt
     4861 ccatcggatc gcgggccacg agcggatcac cggcacgggg ctcgggctgt cgattgcccg
     4921 cgatctctcg cggcgaatgg acggcgacct cgatgtcgcc tcggtgccgg gcacgggatc
     4981 gacgttcgtg ctcgccctcc ccggtccgac cgacgtggcc gcctcggcag tcgcctccgc
     5041 gctcagcgag gcgctcgagc gggaacagca gggcttggag gagttgctcg tacggcgggc
     5101 gatcgacgca ggcaggatcc gcgcggcgcg gtccggcggc gacgacccga cggaacatgc
     5161 ggtgcgccgg tccggcgaca cccgcctgcg cgccctgccg acgctccgtt cggagacgcc
     5221 gtcgccggcc tgatcgggcg gacagtcgac gcgaatcgcc aaacggcaga gacgggcagg
     5281 cccgcgtccg gccgggctgg cgggccccgt cccggccgag ttgtccacag cctgggccgg
     5341 ttcaggtgga ttcgtggata accgtgttga ctccgtcggc cggtccccct agtgtccagc
     5401 atccgcaccg aacccacctt gggtaacgag gctcgcgagg ggcccgggga aacccggccg
     5461 cgaaccggac gagccacgga aaaccggggg ccgagggcgg tgtgacgaac gacgacgccg
     5521 ggagcggagc ccggacggcc cgcgcaggca cgaccgacag gaggggtgcc tggaccgtcc
     5581 gagggacccg ctcccgacgc gcgtcgatct ctgtcctgga ctgcccgcgg cctattcgga
     5641 cgccctcgac gctgccctgg aggcgctcgg aatcgagctg acgggcgatg cgcggaccgc
     5701 gatcgacggg cacatccgcc tgctcctggt atggaacgag gcggtcaacc tgacggcgat
     5761 tcgcgagccc gcagcgatcg ccatccgtca tgtcgtcgac agtctgaccg ggctcgccgt
     5821 cctccaccgt cggggcgtcg accggttcat cgatctgggc agcggcggcg ggttcccggg
     5881 catcacgctg gcggcggcgc tgccggccga ccgggccctg ctcatcgact cgatcggcaa
     5941 gaaggtccgg ttcctcgcca ccgtgatcga cgccatcggc ctgggtgacc acgcggcggc
     6001 tgaggcggtc cgctctgaat cgctcgcggc cagccgcgcc gatcggggtc gctggccggc
     6061 cgtgactgcg cgggcagtcg gaccattggc ggagcttgcc gagctcgccc ttccgttgct
     6121 ttgtcccggc ggggttctcg tcgcctggaa gcgcgtgggc gtggatgacg ccgccgaccg
     6181 tcgagagctc gaggcagcgc ggcgggcgct cgatgcgatc gatcgcgagg cacgcatcga
     6241 tgtcgtggtg gccgttgatg caatgaccgc ccgcgacgcg ccgggcctcg gcgagctcgt
     6301 cgaccaccgc ctcatcgtcg tcgagcgtgg ccccggatcg atcgacccaa cgtggccgcg
     6361 cgagccggcc acgcgacgcc ggagcccatg ttgacgggct caggccgctg ctaggctggc
     6421 tcggtgcgca tcgcggtcct ctccgacatc catgccaacg tcgtcgccct cgactcggtg
     6481 ctcgccgcca tcggctcggt cgacgccgtc tggcagctcg gcgacgtggt cggctacggg
     6541 ccggagcccg acgccgtcgt cgaccgcctg gcggagatcg gagccgtcgg cgtggccggc
     6601 aaccacgacc ttgccgcgct cggcggcacg gagatcgact ggttcaaccc ggatgcgaag
     6661 gccgccatgc tctggacgcg cggccggatg ggcggacgga cgagggcctg gctggcggcc
     6721 ctgccgacga cccgcgtgga gggtggcatg accctcgtgc acggcagccc gcgcgacccg
     6781 gtctgggagt acatcacctc gatgcccgtc gcccgggcga acctaacggt gctcgaaacg
     6841 acgatcggcc tgcacggtca cacccacctg ccgatggcct gggcggatcg tgacggccgg
     6901 atcgacgcga tctcgcccgg tccgggatcg tcgttccgtc tcgatggtcg gccggcgctc
     6961 ctcaacccgg gcagcgtcgg acagccgcgc gacggcgatc ccagcgcgag ctggctggag
     7021 atcgacacgg acgccgaagt ggcaacctgg cgccgggtcg gctacgacat cgaggccgtt
     7081 cggaccgcga tgctcgccgc cggactacca ggtcg
//
LOCUS       DATAIW010000279         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_106645, whole genome shotgun sequence.
ACCESSION   DATAIW010000279 DATAIW010000000
VERSION     DATAIW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106645"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VHM48_08815"
     CDS             <1..560
                     /locus_tag="VHM48_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX2755552.1"
                     /translation="VAVARAPRSGRDGVATEPIAIVPDDVVVETRGAGNATRQISHII
                     APTFPADRLEVVEVYTPSGNWSSWPPHKHDVDDMPGEAVLEEIYHYQFRRPEAWAIQR
                     VYRPDRSRDALFEVRHGDVVIVPDGYHPFSATHGDDAYYLNALAGDRRTMACSFDPDL
                     DWVRATWAGMELDPRVPVVPPRRAG"
     gene            complement(1223..1996)
                     /locus_tag="VHM48_08820"
                     /pseudo
     CDS             complement(1223..1996)
                     /locus_tag="VHM48_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
BASE COUNT          284 a          747 c          734 g          255 t
ORIGIN      
        1 tcgtcgcggt ggcgcgggcg ccacgttccg gacgtgacgg cgtcgccacg gagcccatcg
       61 cgatcgtgcc cgacgatgtc gtggtcgaga cccgcggcgc cggcaacgcg actcgccaga
      121 tcagccacat catcgcgccc acgttcccgg ccgaccgtct cgaggtggtc gaggtctaca
      181 cgccgtcggg caactggtcg agctggccgc cgcacaagca cgacgtcgac gacatgcccg
      241 gcgaggcggt cctcgaggag atctaccact accagttccg tcggccagag gcgtgggcga
      301 tccagcgggt ctaccggccg gaccgctcgc gcgacgcgct gttcgaggtc cgccacggcg
      361 acgtggtgat cgtgcccgac ggctaccacc cgttcagcgc gacccacggc gacgacgcct
      421 actacctcaa cgccctcgcc ggcgaccgcc gaacgatggc ctgctcgttc gatcccgatc
      481 tcgattgggt ccgcgcgacc tgggccggga tggagctcga tccacgcgtg cccgtggtgc
      541 cgcctcgtcg agcgggctga accggggcga cgatcgcggc ggcggttcgg cggcgcaacc
      601 ggtcgagacg gcttgccaga gcccggtcga tcaccggatc gcccagcccg aggcgccgcc
      661 gacgctccgc gatggccgtc gcccgacccg tggcatcgag cgctccttcg agatcgcgga
      721 ggcggtgctc ccggagcttg gcgacctcga tccaggcctg gaccgccacc gcccccggtc
      781 ccgcggcgag atccagccac gtcgcgacgg cctcgtcgtg gcggccaagt cgtcgcaaca
      841 accgtccccg ctcgatcgcg acccgctccg tcgtccacgg tgagtcgagc ctcccggcga
      901 cgagccgctc ccaggacggt cgcggggtcc caccgccgat gtcggccggc cggcgcgggt
      961 gccaccacgg caggccgggt ggttcgtcgg acggctcgag gacggcgcgc tcctcgcttg
     1021 ccgcgatgcg gaccggctcg ctcccgacca ccgtgcgtgc cggttcgggc ctcggtccgg
     1081 caagggcggc gtcgagacag ccgagcgcct cgtcgagacg cccggcccgc ccgaaggacc
     1141 gggcgagacc ggccaggtcg ccgatcggct gtcgccgacg agcctcgcca tcggcgaagt
     1201 cgatgtcgat ccggctcaac aaccgggcga gcgagcggac atcctcgcgg ttgtggcgga
     1261 ccacgtcgat caggggccac gcctcgcccc cgcgcaggaa gtcgaggtac agccccggga
     1321 tctgccagcc atcgacgtcg ccgtggcgga tcgtcccgag gagctcggtc tccaccgtgc
     1381 gcagccgtgc gtcggtcatc cgatggcgga acacccggcg cacgaacggc agcaggtcga
     1441 gatggccggc atggggcggc gccgggaggc gggccatccg gtagcgggcg acgagcagcg
     1501 gccagtcgaa gccgcggccg ttgtatgtca cgagccaggc atcggccggg atgtgggcgg
     1561 ccaggcgggc gagcaggccc ggctcggccg gatggtccgg gagcaggagc tggacctggc
     1621 ggaatcgatc gtcctcccac catccgagac cgacgaggaa cgcgaccgtg cccgccgcag
     1681 tcgcaagccc ggtcgtctcc gtatcgaggc agacgagggg gacctcggcc ggcggcaggc
     1741 cgggcagggt ccgcagtcgg tcgcgatcga tcggcaggac gacggttgcc ggctcgaccc
     1801 gaacgacggg cccggccgga tcgaggatca gctcgccgtc gagcgcgtcg gcaagccgct
     1861 gcccgagctc gatcgagccg cgggcgacgc cgccgtcgat tcgatcgccg ggctccagcc
     1921 gcgtggctgc cggcgccgct gaccggatcg ccgcccgata gttggcgagc cgccgttcca
     1981 gcgtcgcgcc gctcatgccg ggatcgcttc gacgcccggg
//
LOCUS       DATAIW010000280         9256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_245822, whole genome shotgun sequence.
ACCESSION   DATAIW010000280 DATAIW010000000
VERSION     DATAIW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245822"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2590)
                     /gene="alaS"
                     /locus_tag="VHM48_08825"
     CDS             complement(<1..2590)
                     /gene="alaS"
                     /locus_tag="VHM48_08825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX2755553.1"
                     /translation="MSSPVTEHDPRIEPLPASEIRRRFVEFFAERGHTAVPSASLVPP
                     GDATLLFTNSGMVPFKDLLTGAEKRTYNRAVSYQRCLRVAGKHNDFEEVGRTPRHQTL
                     FEMLGNFSFGDYFKREAIHYAWDFLTKELGLPGDRLAITTYKDDEFARQVWRDEIGIP
                     PERMAAWGDVEQGDDHNFWRMADSGPCGPCSEIHFDRGIALSEGPECIPDHSEHCPRW
                     LEVWNLVFMEFDQRPDGRVPLDAPGIDTGMGLERVASAVQQVGSNYDTDLFLPILARL
                     REIVGHDAEAFEQERFSYQVIADHSRAMTFLIADGVLPSNEGRGYVLRRIIRRAVRHG
                     RLLGRRDPFLAAAAGVVIETMSEAYPLLVDRRDAVLAALEREEAQFARTLDAGTTQLE
                     AALRDLAPDAERVIGRRAEDLPVGVPQVPGDLAFRLHDTFGFPIDLTVELAAEYGVGV
                     DRAGFDAALAEQRDRSRSGKKAELAKHAELTSLYQAIQARHGDTTFLGYETTSAPGTV
                     VAILRAGIEFDELTGQGAAEVVLDRSPFYAEGGGQVGDRGELREEGEGSPLFDVEDAQ
                     KLVGGLIVLHGMLHGRLRVGETVDAIVDAERRAHTMRNHTGTHLLHRALRNVVGEAAR
                     QAGSLVTPEYLRFDFPFDRPLTTEELRAIEDQVRSVIRDDRTVTPAFMTMQQAIDAGA
                     DAFFDEKYGETVRTVRVDGFSHELCGGTHCRASGQIGSFAITAERSIGSGMRRIEALT
                     GAGADAYLRSRVVALAAATEAAGAQTAEQLADRIRSLQDELRETRRRLKAGAGTALPK
                     SSDLLARAQEVAPGIRLITFAGPYETIEALKGASKDLRGIFPSGIIALGLDADEPHLF
                     VTVSDDL"
     gene            complement(2647..6114)
                     /locus_tag="VHM48_08830"
     CDS             complement(2647..6114)
                     /locus_tag="VHM48_08830"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755554.1"
                     /translation="MSRSARGPATSFRRPAIAARLRNSAGLAIDLLDNGSVHAIRHGD
                     ILVNQVLGSPLEGGIGNVHLRRRTRAGVTSVPLLGPASTSRFRASDEGATWEGSVDGL
                     DYVCNLRLAPTEPTWFWTIRLANASGRRLSVDLVLAQDLGIATEAAVRTSELYTSQYI
                     DHTVLPDEALGFLICSRQNLAQGTAFPWVMHGCLEGAVGYLTDGFQFYGLAYKASNVP
                     VALGRRRLPNRTYQYEFALPTLQSRAVSLQPGAIGEITFFGAFLADHPDATSTADIGR
                     GRAARMAFRSLRPVTIDEPPRPRSTGFFDAPVLFESHDLGEADLERFFGSEWRHVERR
                     GGALLSFFHGRQQHVVLKAKELVMERPTGHIMRSGRDVLPSDDSLSVTTWMCGVFGSQ
                     VTIGNPSFNKLLGVCRNPLNVLKSSGQRIVLRTDRGDELLGLPSAFEMGPNSARWIYD
                     DDRFTLAIRVTTSLDAPVCRLTVDVVAGGPVELLVSHQIVLGENEDDQPGRMVVDASG
                     GRVELRPDPETRMGRRYPEATFFIVSPDADRIESIGGDRLLHGDGIDRAGAVVVVKTR
                     PISRFSIALTGSVLDARRAEQVASAYGHEPDPDADLESASATFWSELGRQATLGGAAG
                     RWADDLARLNDVVRWYLHDAMIHFTTPHGLEQYSGAAWGLRDVCQGPAEFLAATGHLN
                     ELRDVLRIVYGHQDRQTGDWPQWFMFDRYRDVRAPDSHADIIHWPIKALCDYVEATGD
                     HSILDEHVAYCDDPPLATTAQNGTATEPETIFAHTERQIAVIERDCIPGTALVVFAGG
                     DWEDTLQPVDPDIAGRLVSAWTVELAYQTLDRYRTVCERAGKRRMADRLADLCARMRD
                     DFQRHLLPDGIVAGLAEFGPDRIDYLLHPRDRTTGVAYRLLPMTRGIISGMFTADQAS
                     RHAALIERHLMFPDGARLMDRPMDYHGGPSRIFKRAESAANFGREVGLQYVHAHIRYI
                     ETMARLGRPDEAFRGLLAVCPIGLERDVPAALPRQSNAFFSSSDAAFLDRRQASRQFG
                     RIRSGRVGVKGGWRVYSSGPGIYINQLISNVLGLRAYFDDVVLDPVLPRGADGLTFDV
                     EDDGRPVRYLYHVAGAGFSPRRVDVNGRRLPADRWLDNPYRRGGLLVSRRDFSVALDQ
                     DENLVEIFI"
     gene            complement(6111..7946)
                     /locus_tag="VHM48_08835"
     CDS             complement(6111..7946)
                     /locus_tag="VHM48_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX2755555.1"
                     /translation="MTLAASARIAWFATVGAATERDREAPTDPLPPPEGVRAVQGRGH
                     VTVDWSPVDGAIGYLVHRGPAADGPFAPIDQHTGDLLAVPIGPYLDTTGALGAEAWYA
                     VSSLPTIETEGGILSPPVAVRPGRADAAVAIDVDAGHVVGRIARPWRPIIGSEHLALL
                     RHGPGPGGHDVGAELAEAFRIVRTELGVEAVRAHAILDDSLAVYRETDTGPVHDFALV
                     DDVLARLLDTGLRPIVELSFMPRDLAAEPEATVFSFAGVISPPRDLDRWTDLVRDLVS
                     HLADRFGRAEVRRWAFEIWNEPNLGLFWSGTQSDYFRLYDASVRAVKSVDSTFRVGGP
                     ATAAAGWIDDLLEHCRADEVPIDFISTHTYGMAPLDLRPIVARYGRPDLPLWWTEWGV
                     SSHHGAPINDSAWAAPLVARGMRSAAGRLHALAYWVASDHFVELGEAPALFHGGFGLL
                     TIGNLRKPRFWALAMLNRLGDDELACELDGDGAASLVEAWASADPDGRVAIAVWSGTL
                     DQTRADGEVALDRSISLTIRGLGAATYQLRHHRVDTLHSNIVRTWEDVGRPDWPDADG
                     WARLRAADRLETLGPTRAVAVDGGRLELAFDLPMPAMSLIELVPD"
     gene            complement(7943..>9256)
                     /locus_tag="VHM48_08840"
     CDS             complement(7943..>9256)
                     /locus_tag="VHM48_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX2755556.1"
                     /translation="LSSDGTRSVQTSTTNIGAYLWSTVVAERLGIIGHAEAVRRTEQT
                     IGTLETMERHQASGQFYNWYDHRDGSKLTVWPATGDPMTPILSSVDNGWLAAALQVVR
                     NTVPEVSARAGALFESMDFGFYYRPAVNRIAFHVVPDTGESPCCYDTIVSESRIASYI
                     GIAKGELPAKEYFGAWRTFPDGPVCDWSWQETKPIGVNRTYLGVNVFEGAYPYADFKV
                     VPGWGGSMFEALMPALFVPEEKWGPRSWAVNHPLTVAAQIHHGLDVAGYGYWGFSPSN
                     IPEGGYNAYGVDGIGMNPEGYPSNEDSTFTDAGFAGCRDAKPTPPPSAFTNGVVTPHA
                     AFLALRWAPDATLTNLAAIKSDFASYDQWGFRDSVNVDTGAVSDAYLSLDQGIVMASI
                     GNALADDMLRDAFATKAIRKVLQPIIGMETFGAGPRSAAAAYLNP"
BASE COUNT         1372 a         3165 c         3366 g         1353 t
ORIGIN      
        1 cgaggtcgtc gctgacggtc acgaacaggt gcggttcgtc ggcgtcgagc ccgagggcga
       61 tgatcccgga cgggaagatc ccgcggaggt ccttcgacgc gcccttcagc gcctcgatcg
      121 tttcgtatgg tccggcgaac gtgatgaggc ggatcccggg cgcgacctcc tgcgcccgcg
      181 ccaggaggtc gctcgacttc ggcaacgcgg tcccggcccc ggccttgagc cgccgtctcg
      241 tctcgcgcaa ctcgtcctgg agcgagcgga tccgatcggc gagctgctcc gccgtctggg
      301 cgccggccgc ctcggtcgcg gccgcgagcg cgacgacgcg cgaccgcagg tacgcgtccg
      361 cgcccgcgcc ggtgagggcc tcgatccggc gcatccccga cccgatcgac cgctcggcgg
      421 tgatcgcgaa gctcccgatc tggccggacg cccggcaatg ggttccgccg cagagctcgt
      481 ggctgaagcc gtcgacccgg accgtccgga ccgtctcgcc gtacttctcg tcgaagaacg
      541 cgtcagcccc ggcgtcgatc gcctgctgca tcgtcatgaa cgccggggtc acggtccggt
      601 cgtcgcggat gaccgaccgg acctggtcct cgatcgcccg gagctcctcg gtcgtcagcg
      661 gccggtcgaa cgggaagtcg aaccggaggt actccggcgt cacgagcgag ccggcctggc
      721 gggccgcctc cccgacgacg ttgcggagcg cccggtggag gaggtgggtc ccggtgtggt
      781 tacgcatcgt gtgcgcccgc cgctcggcgt ccacgatcgc gtcgacggtc tcgccgacgc
      841 gcagccggcc gtggagcatg ccgtgcagga cgatcagccc gccgaccagc ttctgggcgt
      901 cctcgacgtc gaacaggggc gatccctcgc cctcctcgcg cagctcgccg cgatcgccga
      961 cctggccgcc gccctcggcg tagaacggcg agcggtcgag gacgacctcg gccgcgccct
     1021 ggccggtcag ctcgtcgaac tcgatcccgg cgcggaggat cgcgaccacc gtcccggggg
     1081 ccgaggtggt ctcgtagccg aggaaggtcg tgtcgccgtg gcgagcctgg atggcctggt
     1141 agagcgaggt cagctcggcg tgcttcgcga gctcggcctt cttgcccgaa cggctgcgat
     1201 cgcgctgctc ggcgagggcc gcgtcgaagc cggcgcggtc gacgccgacc ccgtactcgg
     1261 cggcgagctc gacggtcagg tcgatcggga agccgaacgt gtcgtggagg cggaacgcga
     1321 ggtcgcccgg aacctgcgga acgcccaccg ggagatcctc cgcccggcgg ccgatcaccc
     1381 gctccgcgtc gggcgcgagg tcgcgcaggg cggcctccag ctgggtcgtg ccggcgtcga
     1441 gcgtccgggc gaactgggcc tcctcgcgtt cgagggcggc gaggaccgcg tcgcggcggt
     1501 cgacgaggag cgggtaggcc tcgctcatcg tctcgatcac gacgccggcc gcggcggcca
     1561 ggaacggatc gcgccgaccg agcagccggc cgtggcggac ggcgcggcga atgatccggc
     1621 gcaggacgta gccgcggccc tcgttgctcg gcaggacgcc gtcggcgatc aggaacgtca
     1681 tcgcccggct gtggtcggcg atcacctggt agctgaaccg ctcctgctcg aaggcctcgg
     1741 cgtcgtggcc gacaatctcg cggagccggg cgaggatcgg caggaagagg tccgtgtcgt
     1801 agttcgagcc cacctgctgg acggcgctcg cgacccgctc gaggcccatc cccgtgtcga
     1861 tccccggggc gtcgagcggg acccggccgt ccggccgctg gtcgaactcc atgaagacga
     1921 ggttccagac ctccagccag cgcgggcagt gctcgctgtg gtccgggatg cactccgggc
     1981 cctcgctcag cgcgatgccc cgatcgaagt ggatctcgct gcacgggccg cacggacccg
     2041 agtcggccat ccgccagaag ttgtggtcgt cgccctgctc gacatcgccc catgcggcca
     2101 tccgctccgg cgggatgccg atctcgtcgc gccagacctg acgggcgaac tcgtcgtcct
     2161 tgtaggtcgt gatcgcgagc cgatcaccgg gcagcccgag ctccttcgtc aggaagtccc
     2221 aggcgtagtg gatcgcctcg cgcttgaagt agtcgccgaa cgagaagttg ccgagcatct
     2281 cgaagagggt ctggtggcgc ggcgtccgcc cgacctcctc gaagtcgttg tgcttgccgg
     2341 ccacccgcag gcagcgctgg tagctgaccg cccgattgta ggttcgcttc tcggcgccgg
     2401 tcaggaggtc cttgaacggg accatcccgg agttcgtgaa caggagcgtc gcgtcgcccg
     2461 gggggaccag gctcgcgctc ggcaccgcgg tatggccgcg ctccgcgaag aactcgacga
     2521 agcgtcgacg gatctcggag gcgggcagcg gctcgatccg cggatcgtgc tcggtgacgg
     2581 gggacgacac ggccgcaatg gtaccgaacg gaacggcctc gtcggaccgg gacggccggg
     2641 ttcgcgtcag atgaaaatct cgacgaggtt ctcgtcctga tcgagcgcca cgctgaagtc
     2701 gcgcctcgag acgagcaggc cgccccgtcg atagggattg tccagccagc ggtcagcggg
     2761 cagtcgccgg ccgttgacgt cgacccgccg cggcgagaag ccggcgccgg ccacgtggta
     2821 gagatagcgc accggccggc cgtcgtcctc gacgtcgaac gtgaggccgt cggcgccgcg
     2881 cggcaacacc gggtcgagca cgacgtcgtc gaaatacgcg cgcagtccga gcacattcga
     2941 gatcagctgg ttgatataga tgccgggccc gctggagtag acccgccagc cgcccttgac
     3001 tccgacccga ccgctccgga tccggccgaa ctgccggctg gcctggcggc ggtcgaggaa
     3061 cgcggcgtcg gagctgctga agaaggcgtt gctctggcgc ggaagcgccg cgggcacgtc
     3121 gcgctccaac ccgatcgggc agaccgccag cagcccccgg aacgcttcgt cgggccgacc
     3181 gagccgggcc atcgtctcga tgtatcggat gtgggcgtgg acgtactgga gcccaacctc
     3241 gcgcccgaag ttggccgccg actcggcgcg cttgaagatc cgtgacggtc ccccgtgata
     3301 gtccatcggc cggtccatca gccgcgcgcc gtcaggaaac atgaggtggc gttcgatcag
     3361 ggcggcgtgc cggctggcct gatcggccgt gaacatgcca ctgatgatgc cgcgcgtcat
     3421 cggcagcagg cgatacgcca ctccggtcgt gcgatcgcgc gggtggagga ggtagtcgat
     3481 gcggtcgggc ccgaactcgg cgagtccggc gacgatgccg tcgggcagca ggtggcgctg
     3541 gaaatcgtcc cgcatccggg cgcacaggtc cgccagtcga tccgccatcc ggcgtttccc
     3601 tgcccgctcg cagacggtcc gatagcgatc gagcgtctgg taggcgagct cgacggtcca
     3661 cgcactgacg aggcgcccgg cgatgtccgg gtcgaccggc tggagggtgt cctcccagtc
     3721 gccgccggcg aacacgacca gcgcggtgcc gggaatgcag tcccgctcga tgacggcgat
     3781 ctgccgctcc gtgtgggcga agatcgtctc gggctcggtg gcggtcccgt tctgggcggt
     3841 ggtcgccagc ggcgggtcat cgcagtaggc gacatgctcg tcgaggatcg agtggtcgcc
     3901 cgtcgcctcg acgtagtcgc acagcgcctt gatcggccaa tggatgatgt cggcgtggct
     3961 gtccggggcc cgcacgtcgc ggtagcggtc gaacatgaac cattgcggcc agtcaccggt
     4021 ctgccggtcc tggtgcccgt agacgatccg gagcacgtct cgaagctcgt tcaggtggcc
     4081 ggtggcggcg aggaactcgg ctggaccctg gcagacgtcg cggagccccc atgccgcccc
     4141 gctgtactgc tccaggccgt ggggagtcgt gaaatggatc atcgcgtcgt ggagatacca
     4201 gcgcaccaca tcgttcagac gggcgaggtc gtccgcccat cgcccggccg cgcccccgag
     4261 ggttgcctgc cgcccgagct ccgaccagaa cgtcgctgac gccgactcaa ggtcggcgtc
     4321 gggatccggt tcgtggccgt aggcgctcgc gacctgctcg gctcgccgag cgtcgaggac
     4381 gctgccagtc agggcgatcg agaaccggct gatcggccgc gtcttcacga cgacgacggc
     4441 gccagcgcgg tcgatcccgt ctccatgaag gaggcggtct ccgccgatcg actcgatccg
     4501 gtcggcgtcg ggcgaaacga tgaagaaggt cgcctcgggg taccgacgac ccatccgcgt
     4561 ctcgggatcc gggcgcagct cgacccgccc gccggaggcg tcgacgacca ttcgccccgg
     4621 ctggtcgtcc tcgttctcgc cgaggacgat ctgatgactg acgagaagct cgacggggcc
     4681 gcccgcgacg acgtcgacgg tcaggcggca gaccggggcg tcgagcgacg tcgtcacccg
     4741 gatcgccagc gtgaaccggt cgtcgtcgta gatccagcgc gcgctgttgg ggcccatctc
     4801 gaaggccgac ggaaggccga gcagctcgtc gccccggtcg gtccgcagga cgatccgctg
     4861 gccgctcgac ttgagcacgt tgaggggatt ccggcagacg ccgaggagct tgttgaacga
     4921 gggattgccg atcgtgacct gcgagccgaa gacgccgcac atccaggtcg tgaccgagag
     4981 gctgtcgtcg ctcgggagca cgtcccgacc cgagcgcatg atgtggccgg tcggccgctc
     5041 catgaccagc tccttggcct tcagcacgac gtgctgctgg cggccgtgga agaaggacag
     5101 cagggctccc ccgcggcgct cgacgtggcg ccactccgag ccgaagaacc gctcgagatc
     5161 ggcctcgccg agatcgtggc tctcgaacag gacgggcgcg tcgaagaatc ccgttgagcg
     5221 cgggcgtggt ggctcgtcga tcgtgacggg tcgaaggctc cggaacgcca tccgggcggc
     5281 gcggccgcgt ccgatgtccg ccgtgctcgt cgcgtccggg tggtcggcaa ggaacgcgcc
     5341 gaagaacgtg atctcgccga tcgccccggg ttggagcgaa accgcccgcg actggagcgt
     5401 cggcagcgcg aactcgtatt gatacgtccg gttcggcagc cgcctgcgac cgagggccac
     5461 cgggacgttc gaggccttgt aggccagacc gtagaactgg aacccgtcgg tgagataccc
     5521 gacggccccc tcgaggcagc cgtgcatgac ccacgggaac gccgtgccct gggccaggtt
     5581 ctgcctcgag cagatcagga agccgagggc ctcatccggg aggacggtgt gatcgatgta
     5641 ctggctggtg tacagctcac tcgtccggac ggccgcctcg gtggcgatgc cgagatcctg
     5701 ggcgaggacc agatcgacgc tcaggcgccg tccggaggcg ttggcgagcc ggatcgtcca
     5761 gaaccacgtc ggttcggtcg gggcgaggcg gaggttgcat acgtaatcga gcccgtcgac
     5821 tgatccctcc caggtcgcgc cctcatcgga tgcccggaag cggctcgtcg acgcgggccc
     5881 gagcagcggg accgacgtga cgccggcgcg ggtccgccgc cggaggtgga cgttgccgat
     5941 gccgccctcg agcgggctgc cgaggacctg gttgaccagg atgtcaccgt gccggatcgc
     6001 atggaccgac ccgttgtcga gcaggtcgat cgccagcccg gccgagtttc gcagccgagc
     6061 ggcgatggcc ggccggcgga acgaggtggc cgggcccctg gccgaacggc tcaatcaggt
     6121 acgagctcga tgagcgacat ggcgggcatc ggcaggtcga acgccagctc gaggcggccg
     6181 ccgtcgaccg cgaccgcgcg cgttggtccg agggtctcga gccggtcggc ggcccggaga
     6241 cgcgcccagc cgtcggcatc cggccagtcg ggccgtccga cgtcttccca tgtccggacg
     6301 atgttcgagt ggagggtgtc gacgcggtgg tgtcggagct ggtaggtggc ggctcccaga
     6361 ccccggatcg tgaggctgat cgatcgatcg agggcgacct ccccgtcggc cctggtctgg
     6421 tcgagggtgc cactccagac cgcgatcgcg acccggccgt ccggatcggc cgacgcccac
     6481 gcctcgacga gcgacgcggc tccgtcaccg tccagctcgc aggcaagctc gtcgtcgccg
     6541 agccggttca gcatcgcgag cgcccagaac cgcggcttgc gaagattgcc gatcgtgagc
     6601 aggccgaagc cgccgtggaa caacgccggc gcctcgccaa gctcgacgaa atgatccgat
     6661 gcgacccagt aggccagggc gtggaggcgg ccggccgccg agcgcatgcc ccgggcgacg
     6721 aggggcgcgg cccaagcgct gtcgttgatc ggcgctccgt gatgcgagct gacgccccac
     6781 tcggtccacc acagcggcag gtcgggccgc ccatagcgag cgacgatcgg acggaggtcg
     6841 agcggggcca tgccgtaggt gtgggtcgag atgaagtcga tcgggacctc gtccgcccgg
     6901 cagtgttcga ggaggtcgtc gatccagccg gccgccgccg tcgccgggcc gccgacccgg
     6961 aacgtcgagt cgaccgactt cacggcccgg acgctcgcgt cgtacagccg gaagtagtcg
     7021 gactgggtgc ccgaccagaa cagacccagg ttcggctcgt tccagatctc gaaggcccag
     7081 cggcgcacct cggctcgacc gaagcggtcg gcgaggtggc tgaccaggtc gcggaccagg
     7141 tccgtccagc ggtccaggtc gcgcggcggc gagatgactc cagcgaagct gaacacggtc
     7201 gcctcgggct ccgccgccag gtctcgcggc atgaacgaga gctcgacgat cgggcgcagg
     7261 ccggtgtcga gcaggcgggc aaggacgtcg tcgacgagcg cgaagtcgtg gaccggcccc
     7321 gtgtccgtct cgcggtagac ggcgagcgaa tcgtcgagga tcgcgtgcgc ccggacggcc
     7381 tcaacgccga gctcggtccg gacgatccgg aacgcctcgg ccagctcggc cccgacgtca
     7441 tgcccgccgg gaccgggccc gtggcggagc agcgcgaggt gctcggatcc gatgatcggg
     7501 cgccacggcc gggcgatccg gccgacgaca tgtccggcgt cgacgtcgat cgcgacggcc
     7561 gcgtccgctc gcccgggacg cacggccacc ggtggcgaga ggatcccgcc ctcggtctcg
     7621 atcgtgggca ggctcgagac ggcgtaccac gcctccgcac cgagcgcgcc ggtcgtgtcg
     7681 aggtacggcc cgatcgggac cgccagcagg tctccggtgt gctggtcgat gggcgcgaat
     7741 ggaccgtccg cggccgggcc ccggtggacg agatagccga tcgcgccatc gaccggggac
     7801 cagtcgacgg tgacgtggcc gcggccctgg acggcccgga ccccctccgg tggcgggagg
     7861 gggtccgtcg gcgcctctcg atcgcgctcg gtcgccgcgc cgaccgtcgc gaaccaggcg
     7921 atccgggccg aagcggccag ggtcacgggt tcaggtaggc ggcggccgcg ctgcgcggtc
     7981 cggccccgaa cgtctccatg ccgatgatcg gttgaaggac cttgcggatc gctttcgtcg
     8041 cgaatgcgtc gcgcagcatg tcgtccgcga gggcattgcc gatcgaggcc atgacgatcc
     8101 cctggtcgag cgagaggtaa gcgtcggaca cggcgccggt gtcgacgttg accgagtcgc
     8161 ggaagcccca ctggtcgtag ctcgcgaagt ccgacttgat cgcggccagg tttgtcaggg
     8221 tcgcgtcggg cgcccagcgc agggcgagga acgcggcgtg cggagtgacg accccgttcg
     8281 tgaacgcgga gggcggcggc gtcggcttgg cgtcgcgaca cccggcgaag cccgcgtcgg
     8341 tgaacgtgct gtcctcgttc gagggatagc cttccggatt catcccgatc ccgtcgacgc
     8401 cgtaggcgtt gtagccgccc tccgggatgt tcgacggcga gaagccccag tagccatacc
     8461 ccgccacatc gagcccgtgg tggatctggg ccgcaacggt cagggggtgg ttcaccgccc
     8521 agcttcgcgg gccccacttc tcttcaggca cgaacagcgc cggcatcagg gcctcgaaca
     8581 tgctcccgcc ccaaccgggg acgaccttga agtccgcgta cgggtaggcg ccctcgaaga
     8641 cgttgacgcc gaggtaggtc cggttgacgc caatcggctt ggtctcctgc cagctccagt
     8701 cgcagacggg gccatcgggg aaggtccgcc acgcgccgaa gtactccttg gcgggcagct
     8761 cgcccttggc gatcccgatg tagctcgcga tccggctctc gctgacgatc gtgtcgtagc
     8821 agcacggcga ctcgcccgtg tcgggcacga cgtggaaggc gatgcgattg acggcgggcc
     8881 ggtagtagaa gccgaagtcc atgctctcaa agagcgcgcc ggcccgggcc gagacctccg
     8941 ggacggtgtt ccggacgacc tgcagggcgg ccgccagcca gccgttgtcg accgaggaca
     9001 ggatcggggt cattgggtct ccggtcgccg gccagaccgt cagcttcgac ccgtcgcggt
     9061 ggtcgtacca gttgtagaac tggccgctcg cctgatggcg ctccatcgtc tcgagcgtcc
     9121 cgatcgtctg ctcggtgcgc cggacggcct cggcatggcc gatgatcccg agccgttcgg
     9181 cgacgacggt gctccagagg taggcaccga tgttcgtcgt ggaggtctgg acactgcgcg
     9241 tgccgtccga gctgag
//
LOCUS       DATAIW010000281         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_17101, whole genome shotgun sequence.
ACCESSION   DATAIW010000281 DATAIW010000000
VERSION     DATAIW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17101"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHM48_08845"
     CDS             complement(<1..243)
                     /locus_tag="VHM48_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2755557.1"
                     /translation="MAKVTMPQLGESVAEGTVGKWLKQVGDTVEKYEPLLEVITDKVN
                     AEVPSPFEGILTEILAEEGATVPNDAEIAIIDTGDEA"
     gene            362..697
                     /locus_tag="VHM48_08850"
     CDS             362..697
                     /locus_tag="VHM48_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX2755558.1"
                     /translation="MLRPFRQVDVFTSEPYRGNPVAVVHDADGIDEATMLRFAQWTNL
                     SETTFLLPPTTPGADDRVRIFTPISEFQFAGHPTLGTCHAWLEAGGVPRDPAAIFQEC
                     GSGLIRIRR"
     gene            complement(893..1876)
                     /locus_tag="VHM48_08855"
     CDS             complement(893..1876)
                     /locus_tag="VHM48_08855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2755559.1"
                     /translation="MALKTFIEAIRDGLRDEMARDENVIILGEDVGKKGGVFLVTDGL
                     WAEFGDDRVIDTPLTESQIIGTSIGAAIGGLRPVAEIQFADFIHPAFNQLVSEAARLR
                     YRSNNGYSAPMVVRAPYGGGVHGALYHSQSVEAFYTHVPGLKVVIPSTPYDAKGLLRS
                     CIRDDDPVLFFEHKKMYRSVRGDVPDGEYTVPLGRAQITRPGTQVTVIAYGLMAHYAL
                     EAADRVADDGISVEVVDLRTLRPLDKETVLDSVRKTGKCLVVYEDNRFGGYGAEVAAT
                     IGEEAFDYLDGPVTRIAGPDVPGVPYNHVLEDWFMVNPEKIADGIRKLAAY"
     gene            complement(2094..3230)
                     /locus_tag="VHM48_08860"
     CDS             complement(2094..3230)
                     /locus_tag="VHM48_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2755560.1"
                     /translation="MAVTERSGTSPAAPLGADLGLTTDDLLEMYRLVALARAVDERMW
                     ILNRAGRIPFVISGQGHEGAQVGITWALQKGKDWIAPYYRSIATCLSFGMSARDIMTA
                     QYATASDPSSGGRQMPGHYGSHEHNLVSVSSPVATQLLHAVGLALAAKIRKTGQVALT
                     IMGEGSSNQGEVHEGLNFAAIHQLPFIFIVENNGYAISVPLARQVAGSSVAQRAAGYG
                     IPGVTVDGSDVLACYAAAREAVARARAGDGPTLIEAKVTRLTGHSSDDQQTKYRSAEE
                     LAADKSLDPVPKFRAQLVEAGVLTEEAEERIDAAIKEAVHDATEYAESQPDPDPSTAM
                     KWVYAEDWPSEMPPPWGLGDAAAPATDAAAPATDASMPAGEGAH"
     gene            complement(3230..4672)
                     /gene="lpdA"
                     /locus_tag="VHM48_08865"
     CDS             complement(3230..4672)
                     /gene="lpdA"
                     /locus_tag="VHM48_08865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX2755561.1"
                     /translation="MAVGSSNDFDLVVLGAGTGGYTAAFRAAQLGLKVALVDEDKIGG
                     TCLHRGCIPTKALLESAGFVEHVRHAKEYGLTIPGETGIDYVQVAARKDQVVKRMWTG
                     LKTLIDKNKVTWVAGRGRLEGPNKVRVTQPGEDGTPGGGGDRVLQATDIILATGSRVK
                     SLPGLTPDGERILTSDDVLRSTTIPKDIVIVGAGAVGCEFASMYHDLGTKVTLLEYFP
                     QIVPLEDREVSQALERSFTRRGMTVMTNARFDSTKVEVTKDGICLAVGPEGKEPTELR
                     AEQLLVATGRATNVEEIGLETTKVEVEKGFIKVDGRMRTKEQHVYAIGDVVGGLMLAH
                     TAAHEGIVAAHIIAGDKDVHPIDYVKQPRATYCRPEIASIGLTQQQAEERGRPIKVGK
                     VPFQAIAKAIIGGEYEGFAKIIADKETDETLGIHLIGPHATDLIAEASVAFELEATPW
                     EIGGATHAHPTLSEILGEAAMAVDGRSINF"
     gene            4848..>5051
                     /locus_tag="VHM48_08870"
     CDS             4848..>5051
                     /locus_tag="VHM48_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2755562.1"
                     /translation="MDGATLLLDLALIVGAYLCGSIPMGVVIARLTGGVDPRTVGSGR
                     TGGTNALRALGRKRAGIVVTGDLL"
BASE COUNT          725 a         1751 c         1733 g          842 t
ORIGIN      
        1 cgcctcgtca cccgtgtcga tgatcgcgat ctcggcgtcg ttgggcaccg tggccccttc
       61 ctcggccagg atctccgtga ggatgccctc gaacggcgac gggacctcgg cgttgacctt
      121 gtcggtgatg acctcgagga gcggctcgta cttctcgacc gtgtcaccga cctgcttgag
      181 ccacttgccg accgtcccct cggcgacgct ctcaccgagc tggggcatcg tcaccttggc
      241 cactctgtcg ttcctccggg tcgccgggcg gatcgtgccc gccatcgcgt tgttcggcgc
      301 ctagtgtcgc gcatcgagcc gggaagcgcc gtgcggcagc gcccgttggc agactcggcc
      361 gatgctccgc ccattccgcc aggtcgacgt cttcacctcc gagccctacc gcggcaaccc
      421 ggtcgcggtc gtccacgacg ctgacggcat cgacgaggcg acgatgcttc gcttcgccca
      481 gtggacgaat ctctcggaga cgaccttcct gcttccgccg acgacacctg gcgccgacga
      541 tcgggtccgc atcttcactc cgatctcgga gttccagttc gccggccatc cgacgctcgg
      601 gacgtgccac gcctggctcg aggccggcgg ggttccccgt gacccggcgg cgatcttcca
      661 ggagtgtggc tccggactca tccggatccg gcggtgagtc aggatgatcg cccgcagacg
      721 ggcatggatc gcgtcgaact ccggccgggc cgatcgcgaa cggggtcgcg taggccccag
      781 aggacgccgg ccaacgtcga gacggggaac aagatcgggt cgaagccgcc gaccacgaac
      841 agcacgccga tgacggcgcc gagcggggca ccgccaacgt tcgcgccgac catcagtacg
      901 ccgcgagctt gcggatgccg tcggcgatct tctcggggtt gaccatgaac cagtcctcga
      961 ggacgtggtt gtagggcacc cccgggacgt ccggcccggc gatccgcgtc accggcccgt
     1021 cgagatagtc gaacgcctcc tcgccgatcg tggcggcgac ctccgccccg tacccgccga
     1081 accggttgtc ctcgtagacg acgaggcact tgccggtctt ccgcacggag tcgagcacgg
     1141 tctccttgtc gagcggccgg agcgtccgca ggtcgacgac ctcaacgctg atcccgtcgt
     1201 cggcgacgcg gtccgcggcc tcgagagcgt aatgggccat caggccgtag gcgatgaccg
     1261 tgacctgggt ccccgggcgg gtgatctggg cgcggcccag cggaaccgtg tactcgccgt
     1321 cggggacgtc gccgcgcacg ctccggtaca tcttcttgtg ctcgaagaag agaacgggat
     1381 cgtcgtcgcg gatgcagctc cggaggaggc ccttggcgtc gtacggggtc gacgggatga
     1441 caaccttgag gcccgggacg tgggtgtaga acgcctcgac cgactgcgag tgatagaggg
     1501 cgccgtggac gccgccgccg tacggcgcgc ggacgaccat cggcgccgaa tagccgttgt
     1561 tcgagcgata gcggaggcgc gccgcctccg agacgagctg gttgaacgcg ggatggatga
     1621 agtcggcgaa ctggatctcg gcgaccggcc ggagcccgcc gatcgcggcc ccgatcgagg
     1681 tcccgatgat ctggctctcg gtcagcgggg tatcgatgac ccggtcgtca ccgaactcgg
     1741 cccacaggcc atcggtcacg aggaagacgc cgcccttctt gccgacgtcc tcgccgagga
     1801 tgatgacgtt ctcgtcgcgg gccatctcat cgcggagacc gtcgcggatc gcctcgatga
     1861 acgtcttgag ggccatcagg ccaccagccc ggcgcgggtg atgtccgcca ggcattgatt
     1921 ccagaccgag gccgatggtt tcggtcgctt ggggttgcca acgggccgat tcgaggccga
     1981 atgatgccgg tcgcgcatca attcgaccgc agacgacgcc gcggacgacc ttcgcatcga
     2041 aaacaatgcc tgtgagacat cgggctcgtg gtgggatacg ccttggacgg ccatcagtgc
     2101 gcgccctctc cggccggcat tgaggcatcg gttgccggag cggcggcatc ggttgccgga
     2161 gcggcggcat cgcccagtcc ccacggcggc ggcatctcgg agggccagtc ctcggcgtag
     2221 acccacttca ttgcggtcga cgggtccgga tccggctggg attcggcgta ctccgtcgcg
     2281 tcgtggactg cctccttgat cgcggcgtcg atgcgctcct cggcctcctc ggtcaggacg
     2341 ccggcctcga cgagctgggc ccggaacttc ggcacagggt ccagggattt gtccgccgcg
     2401 agctcctccg cggagcggta cttcgtttgc tggtcgtcgc tcgaatggcc ggtcaggcgg
     2461 gtgaccttgg cctcgatgag cgtcgggcca tcgccggcgc gggcgcgcgc caccgcctcg
     2521 cgggcggccg cgtagcaggc caggacgtcc gaaccgtcga cggtcacccc ggggatcccg
     2581 tagccagccg cccgctgggc gaccgacgac cctgccacct ggcgggccaa cggcaccgag
     2641 atggcatagc cgttgttctc gacgatgaag atgaacggca actggtggat cgcggcgaag
     2701 ttcagccctt catggacctc gccctgattc gacgatccct cgcccatgat ggtcagcgcg
     2761 acctggccgg tcttgcggat cttcgccgcc agcgccaggc caaccgcgtg gaggagctgg
     2821 gtcgcgaccg gcgacgacac cgacacgagg ttgtgctcgt gagagccgta gtgaccgggc
     2881 atctggcgac cgcccgacga cggatccgac gccgtcgcgt actgggcggt catgatgtcg
     2941 cgggcgctca tcccgaagct caggcaggtc gcgatcgagc ggtagtaggg ggcgatccag
     3001 tccttgccct tctggagcgc ccaggtgatc ccgacctgcg ccccttcatg gccctggccg
     3061 ctgatcacga acggaatacg ccccgcgcgg ttgaggatcc acatccgctc gtcgacggcg
     3121 cgcgcaaggg cgaccaagcg gtacatctcg agcaggtcgt cggtggtcag gccgaggtcg
     3181 gcgccgagcg gcgccgcggg cgacgtgccg gagcgctcgg tcacggccat cagaagttga
     3241 tcgaccgacc gtcgaccgcc atcgccgcct cgccgaggat ctccgacagc gtcggatgag
     3301 cgtgcgtcgc tccgccgatc tcccacggcg tcgcctcgag ctcgaacgcc accgacgcct
     3361 cggcgatcag gtcggtcgca tgcggcccga tcaggtggat gccgagagtc tcgtcggtct
     3421 ccttgtcggc gatgatcttg gcaaagccct cgtactcgcc gccgatgatc gccttggcga
     3481 tggcctggaa cgggaccttg ccgaccttga tcggccgacc gcgctcctcg gcctgctgct
     3541 gggtcagccc gatcgaggcg atctccggcc ggcagtaggt cgcccgcggc tgcttcacgt
     3601 agtcgatcgg gtggacgtcc ttgtcgccgg cgatgatgtg ggcggcgacg atcccttcgt
     3661 gcgcggcggt atgagcgagc atcaggccac cgacgacgtc gccgatcgcg tagacgtgct
     3721 gctccttggt ccgcatccgg ccgtcgacct tgatgaagcc cttctcgacc tcgaccttcg
     3781 tcgtctccag cccgatctcc tcgacgttgg tcgcccgccc ggtggcgacg aggagctgct
     3841 cggcgcgcag ctccgtgggc tccttgccct ccggcccgac cgccaggcag atcccgtcct
     3901 tcgtcacctc gaccttggtc gagtcgaagc gggcgttcgt catcacggtc atgccgcgcc
     3961 gggtgaagct ccgctcgagg gcctggctga cctcgcgatc ctcgagcggc acgatctgcg
     4021 ggaagtactc gagcagcgtc accttcgtcc cgaggtcgtg gtacatcgag gcgaactcgc
     4081 agccgaccgc gcccgccccg acgatcacga tgtccttcgg gatggtcgtc gagcggagga
     4141 cgtcgtcgct cgtcaggatc cgctcgccgt ccggggtcag gccgggcagg gacttcaccc
     4201 gggagcccgt ggcgaggatg atgtcggtcg cctggaggac ccggtcgccg ccgccgccgg
     4261 gcgttccgtc ctcgccgggc tgagtgaccc ggaccttgtt cgggccctcg aggcggccac
     4321 gaccggcgac ccacgtcacc ttgttcttgt cgatcagggt cttgagaccc gtccacatcc
     4381 gcttcacgac ctggtccttg cgggccgcca cctggacgta gtcgatgccc gtttcgcccg
     4441 ggatcgtcag gccgtattcc ttggcatggc ggacgtgctc gacgaagccg gccgactcga
     4501 gcagcgcctt cgtcgggatg cagccgcggt ggagacaggt cccaccgatc ttgtcctcgt
     4561 cgacgagagc gaccttcagc cccagctggg ccgcccggaa tgccgccgtg tagccgccgg
     4621 tgcccgcgcc gagcacgacg agatcgaagt cattggacga acctacggcc atgggatcag
     4681 tgcgcctcgc gggccacggt ccggcgccct cggggcgctc gggtgggcgg gtggatcgtg
     4741 tgcgccgatg gtacgcgggc cctcgcaaag cccgcaaccg gaccccgggg gcggcagtgc
     4801 tagactcgcc gccaatcggc ccggttctgc tgcatccgcg gagcgacatg gacggcgcga
     4861 ctcttctcct cgatctcgcg ctcatcgtcg gggcctacct gtgtggctcg atcccgatgg
     4921 gcgtcgtcat cgcccggttg accggcgggg tggatccgcg gaccgtcggg tcggggcgaa
     4981 ccggcggcac gaacgcgctg cgggcgctcg ggcgaaagcg ggcggggatc gtcgttaccg
     5041 gcgacctgct c
//
LOCUS       DATAIW010000282         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_186533, whole genome shotgun sequence.
ACCESSION   DATAIW010000282 DATAIW010000000
VERSION     DATAIW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186533"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHM48_08875"
     CDS             <1..264
                     /locus_tag="VHM48_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755563.1"
                     /translation="GDGATIGFAGVGVTGVSGSPFAATDAEAALVGQPPSEAVFRAAG
                     QAAAAMSQPVSDLRGPVDYKRAMVAELTVRSLRLAVERALAHA"
     gene            354..902
                     /locus_tag="VHM48_08880"
     CDS             354..902
                     /locus_tag="VHM48_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2755564.1"
                     /translation="MTTQHVAMTVNGEKREADVEPRLLLVHLLRDVFLLTGTHIGCDT
                     SNCGACTVHVNGQSAKSCTMLAVQADGAEIKTIEGMADGAKLHPLQQAFWDQHGLQCG
                     FCTPGMIMQAAWFLGENPDPSETEIRAGIAGNLCRCTGYVNIVKSIQQVAGTLAEGAS
                     APVVAAATTEGQAVSAEPAAAG"
     gene            918..3287
                     /locus_tag="VHM48_08885"
     CDS             918..3287
                     /locus_tag="VHM48_08885"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX2755565.1"
                     /translation="MTTETPLQTTPEVGGMGHSVKRKEDPRFIRGQGKYIDDLVLPGM
                     LWLDIVRSPYAHAKIKGIDASEALKVPGVAAVITGADLEKAGLHWMPTLAGDRQMVLP
                     TDTVMYQAQEVAAVVATSRYAAADGVAAVLVDYEPLEVIVDPFKALEEGAMVLRPDRA
                     ADKQTNRIWHWESGDKAKADAALAAADVRIEEDIYIPRIHVASIETCGCVADWDATNG
                     HLDLHMTSQAPHAIRTVLALVSGLPEHQIRVKTHDIGGGFGGKVPVYPGYVLAVVASL
                     TTGKPVKWIEDRSENLQADSFARDYHIHAELAATKEGKITGLKVRTIADHGYTDAAAD
                     PSKFPAGLFNVITGSYDFQNCFVEVDGVYTNKPPGGVAYRCSFRVTEAVHAIERMVDI
                     LAHDIGKDPAQLRMENFIQPEQFPYKTPTGWSYDSGNYPAALQKAMDIIDYAGLRKEQ
                     AEKLAKGELMGIGISSFTEIVGAGPSHDFDILGIKMFDSCEIRVHPTGKVIARLGVQS
                     QGQGHETTFAQIIAEELGFPVADIKIEEGDTDTAPYGLGTYASRSTPVAGAATAIAAR
                     KIKEKARHIAAHLLECSADDLEWTNGKWSVKGSPDRFKTIQEIAFAAYTNHPAGMEAG
                     LEAVDYYDPPNLTFPFGSYICVVDIDKGTGEVHVRRFVAIDDCGNIINPMIVDGQIHG
                     GLTMGLAPALYEQITYDEQGNNLAGTFMDYLLPTAVETPAWEVGKTTTPSPHHPIGAK
                     GVGESATVGAPPAIANAVVDALWHLGVRNIDIPITPQKVWAILHEKGIS"
     gene            3421..4500
                     /locus_tag="VHM48_08890"
     CDS             3421..4500
                     /locus_tag="VHM48_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2755566.1"
                     /translation="MSRSIFARAADLEAAGRPFVVATVVAVARPTSARPGATGIVHPD
                     GTIEGWVGGSCAQPVVVREALHALADGQPRLLRLSKDNPAEGRRADGIVELVMTCHSG
                     GTLEIYVEPHLPAPLLWIAGTTPIAGALAALGAVSGWRVSVFDPVAIGEDYPAAERVS
                     QETALGRVDPEASPYLVVATQGVWDEEALAAGLRRNASYVGLVASPTRANVVRQWLRV
                     ETGLADERLAALRAPAGMDIGAETAAEIAVSILAELVQVRRGRADFVAAPGPATLAGF
                     PDGAGVTPLQPVVDDIVLLDPVCGMTVDRATARHLAEHDGTVYAFCSIGCRTRFIKDP
                     PGFLAAPGTAGMATTESDAPVSSDA"
BASE COUNT          774 a         1717 c         1587 g          653 t
ORIGIN      
        1 ggcgacggcg cgaccatcgg tttcgccggg gtcggcgtca cgggggtctc cggatcgccc
       61 ttcgcggcga cggacgccga ggcggcgctc gtgggccagc cgccatccga ggcggtgttc
      121 cgggccgccg gccaggccgc cgccgcgatg agccagcccg tctccgacct ccgcggcccc
      181 gtcgactaca agcgggccat ggtcgccgag ctcaccgttc gatcgctgcg cctcgcggtc
      241 gagcgcgccc tcgcccacgc atagcgggag ccgacgacga tgaccaccca tcctcgggcc
      301 aatggcctcg cggagctcag ccttggacct cggagcgggg agatgagaat ccgatgacca
      361 cccagcacgt tgccatgacg gtcaacggcg agaagcggga ggccgacgtc gagccgcgcc
      421 tcctcctcgt ccacctcctc cgtgacgtct tcctcctgac cggcacccat atcggctgcg
      481 acacgagcaa ctgcggcgcg tgcaccgtcc acgtcaacgg ccagagcgcc aagtcgtgca
      541 cgatgcttgc ggtccaggcg gacggcgccg agatcaagac gatcgagggc atggccgacg
      601 gggccaagct ccacccgctc cagcaggcgt tctgggacca gcacggcctc cagtgcgggt
      661 tctgcacgcc gggcatgatc atgcaggcgg cctggttcct gggcgagaac ccggacccgt
      721 cagagaccga gatccgcgcc gggatcgccg gcaatctctg tcgctgcacg ggctacgtga
      781 acatcgtgaa atcgatccag caggtcgcgg gcaccctcgc cgagggcgcg tccgcaccgg
      841 tcgttgccgc cgcgacgacc gaaggccagg ccgtctcggc tgaaccggcc gcggccggct
      901 gagcggggag acatccgatg accaccgaga cgccgctcca gaccaccccc gaagtcgggg
      961 gcatgggcca ttccgtcaag cgcaaggagg atccgcggtt catccgcggc cagggcaaat
     1021 acatcgacga tctggtcctg ccgggcatgc tctggctcga catcgtccgg agcccgtatg
     1081 cccacgcgaa gatcaagggg atcgacgcca gcgaagcgtt gaaggtcccg ggggtggcgg
     1141 ccgtgatcac gggcgccgac ctcgagaagg ccggtctcca ctggatgccg accctcgccg
     1201 gcgaccgcca gatggtcctg ccgaccgaca cggtcatgta ccaggcccag gaagtggccg
     1261 ccgtcgtcgc cacctcgcgc tacgcggcag ccgacggcgt cgccgcggtc cttgtcgact
     1321 acgagcccct cgaggtgatc gtcgatccgt tcaaggcgct cgaggaaggc gcgatggtcc
     1381 ttcgcccgga ccgggcggcg gacaagcaga ccaaccgcat ctggcactgg gaatccggcg
     1441 acaaggccaa ggccgacgcg gccctcgcgg cggcggacgt ccggatcgag gaggatatct
     1501 acatcccccg gatccacgtc gcctcgatcg agacgtgcgg ctgcgtcgcc gactgggacg
     1561 ccacgaacgg tcacctcgac ctgcacatga cgagccaggc gccgcacgcc atccggacgg
     1621 tgctcgcgct cgtctcgggc cttccggagc accagatccg ggtcaagacc cacgacatcg
     1681 gcggcgggtt cgggggcaag gtgccggtgt atcccgggta cgtcctcgcg gtcgtcgcca
     1741 gcctgaccac cggcaagccg gtgaagtgga tcgaggatcg ctcggagaac ctccaggccg
     1801 attcgttcgc ccgcgactac cacatccacg ccgagctcgc ggccaccaag gaaggcaaga
     1861 tcaccggcct caaggtccgc acgatcgccg atcacggcta caccgatgcc gccgccgacc
     1921 cctcgaagtt cccggccggg ctgttcaacg tgatcaccgg ttcctacgac ttccagaact
     1981 gcttcgtcga ggtcgacggc gtctacacga acaagccgcc gggaggcgtg gcctaccgct
     2041 gctcgttccg ggtgaccgag gcggtccacg cgatcgagcg gatggtcgac atcctcgccc
     2101 acgacatcgg caaggacccg gcccagctcc ggatggagaa cttcatccag cccgagcagt
     2161 tcccctacaa gacgccgacc ggctggtcgt acgattcggg gaactacccg gccgcgctcc
     2221 agaaagcgat ggacatcatc gactacgccg ggctgcgcaa ggagcaggcg gagaagctgg
     2281 ccaagggcga gctgatgggg atcgggatca gcagcttcac cgagatcgtc ggcgcgggcc
     2341 cgtcccacga cttcgacatc ctcgggatca agatgttcga ctcctgcgag atccgggtcc
     2401 acccgaccgg caaggtcatc gcccggctcg gcgtccagag ccagggccag ggccacgaga
     2461 cgaccttcgc ccagatcatc gccgaggagc tcggcttccc cgtcgccgac atcaagatcg
     2521 aggaaggcga caccgacacc gcgccctacg ggctcgggac gtatgcgtcg cgctcgacgc
     2581 cggtcgccgg cgcggccacg gcgatcgccg cccgcaagat caaggagaag gcccgccaca
     2641 tcgccgccca cctgctggaa tgcagcgccg acgacctcga gtggaccaac gggaagtgga
     2701 gcgtcaaggg ctcgcccgac cggttcaaga cgatccagga gatcgcgttc gcggcctaca
     2761 cgaaccatcc ggccgggatg gaggccgggc tcgaggcggt cgactactac gacccgccga
     2821 acctgacgtt cccgttcggc tcgtacatct gcgtcgtcga catcgacaag ggcaccggcg
     2881 aggtccacgt ccggcgattc gtcgcgatcg acgactgcgg caacatcatc aacccgatga
     2941 tcgttgacgg ccagatccac ggcggcctga cgatgggcct cgccccggcg ctctacgagc
     3001 agatcacgta cgacgagcag ggcaacaacc tcgccgggac gttcatggac tacctcctcc
     3061 cgacagcggt cgagacgccc gcctgggagg tcggcaagac gacgacgccg tcgccgcacc
     3121 acccgatcgg ggcgaagggg gtcggggagt cggcgaccgt cggcgccccg ccggcgatcg
     3181 ccaacgccgt cgtcgacgcc ctctggcacc tcggcgtacg gaacatcgac atcccgatca
     3241 ccccgcagaa ggtctgggcg atccttcacg agaagggcat ctcctgatcg tgccgccggt
     3301 ctcaccgatc cgagccgccg tcgccgtcgg aacgcccagc gttcctggcg gcgaacggcg
     3361 ggccagcctc gtcgtcgacg tgggacgaac gatcagtgtt caatccgagg cgcggaaccg
     3421 gtgagccgca gcatctttgc ccgggcggcc gacctcgagg cggccggtcg cccgttcgtc
     3481 gtcgcgacgg tcgtcgcggt ggcccgaccg acatcggccc gccctggtgc gaccgggatc
     3541 gttcacccgg acggaacgat cgagggctgg gtcggaggca gctgcgccca gccggtcgtc
     3601 gtccgcgagg cgctccatgc cctcgccgac ggccagccgc ggctgctccg cctatcgaag
     3661 gacaatccgg cggagggccg ccgggccgac gggatcgtcg agctcgtcat gacctgccac
     3721 tccggtggca ccctggagat ctacgtggaa ccgcatctgc ccgcgccgct cctgtggatc
     3781 gccgggacga caccgatcgc cggcgcgctg gccgccctcg gtgcggtgtc cggctggcgg
     3841 gtctcggtgt tcgatccggt cgccatcgga gaggattacc cggccgccga gcgggtcagc
     3901 caggagacgg ccctcgggcg ggtcgatccc gaggccagcc cgtacctcgt cgtcgcgacc
     3961 cagggcgtct gggacgagga ggccctcgct gccggactcc gccgcaacgc ctcgtacgtc
     4021 ggcctcgtcg cctcgccgac gcgagcgaat gtcgtccgcc agtggctccg cgtggaaacc
     4081 ggtcttgccg acgagcggct cgccgccctg cgcgccccgg ccgggatgga catcggggcc
     4141 gagacggccg cggagatcgc cgtctcgatc ctcgccgagc tcgtccaggt gcgccgcggc
     4201 cgggcggact tcgtcgcggc gcccggcccc gcgaccctcg ccgggttccc cgacggcgcg
     4261 ggcgtcaccc cgctccaacc ggtcgttgac gatatcgtcc tgctcgaccc ggtctgcggg
     4321 atgacggtcg atcgggcgac ggcgcgtcac ctcgccgagc atgacgggac ggtctacgcg
     4381 ttctgctcga tcggctgccg gacccggttc atcaaggatc cgccaggctt cctcgcggcg
     4441 cccggcacgg ccgggatggc gaccaccgaa tccgacgcgc cggtatcatc ggacgcctga
     4501 ccgggcaggc gccccggttg ccactcccgc cggctcagcc ggcgtctcgc tgatccggag
     4561 gtccgcatga agttcgccgg aacggtcgac atcgccgcgc cccgcgccaa ggtctgggcg
     4621 ttcgtcatcg atccgaacca ggtcggccag tgcggccccg gcgtcgagtc gatcgaggtc
     4681 atcgatgaca cccatttcaa ggcgaccgcc aaggtcggga tcgggttcat c
//
LOCUS       DATAIW010000283         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_77692, whole genome shotgun sequence.
ACCESSION   DATAIW010000283 DATAIW010000000
VERSION     DATAIW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77692"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..660
                     /locus_tag="VHM48_08895"
     CDS             178..660
                     /locus_tag="VHM48_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX2755567.1"
                     /translation="MVLVALWMRRTPPPDGQIGETRTIDPSGDGIAPRRRWHRFGRRP
                     APTTAVAAYVALVGDLDRHPNVRREPAETPAAHAARLRADGLAELSLDLLAADYALAR
                     YAGVTLPEREDRRAVGRWRGLRRRLITHRDGPVRPGPGSSVPTPDTEPPLDLEPRRTF
                     "
     gene            complement(709..1953)
                     /locus_tag="VHM48_08900"
     CDS             complement(709..1953)
                     /locus_tag="VHM48_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755568.1"
                     /translation="MTFVAGAALSLYWIDFNLYLASLGLSTATIGVVSTIASIAGAAV
                     AFPASAASDRFGRRTIIAAGIAVCLVGLVGLLLTQALALIVVFAALWSVGQQSLQVVQ
                     APFLTEHSEPEHRNELFAAQFAIQNVTNVVAAVLGAVGAAAIASLIGLDPKGPGTYRV
                     ILVVMVVLLVASLAIVALLTDDRPRVAAGPRLVGLGEPAAFPPDPRRSRARLGIVIRD
                     RGRFARLLFPGLLISIGAGQVIPFLNIFIQRKFGLDLTSLNAVFAFTSLGTVAAILAQ
                     PTLARRFGQITSVVIVQAASIPFLAVLGFSPLIWLVILAMAVRNSLMNAGNPIFTAFA
                     MEQVTPAERASLSAAMSVLWQIGWVIGGTWYAVLQAVLGFEAGYTVNFVTIITLYSIA
                     TGLYWIWFRAADRRALATRLAA"
     gene            2260..>2768
                     /locus_tag="VHM48_08905"
     CDS             2260..>2768
                     /locus_tag="VHM48_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755569.1"
                     /translation="MRKSTSGSSSVTVLRQIETTSPFRGLGRLPDEWRSELLALGDED
                     LGRAITILLHHAAALPSAAAAGASEAIDELVGGGPIDRLRPGLDPADLVAVFAAISRD
                     ASRQDLTRVVVRLLQLFDPDDVLAVVGVDARSLDLRARLSGEFAQAAMAPPPDDKPHS
                     RGIDFDQFG"
BASE COUNT          436 a          996 c         1008 g          328 t
ORIGIN      
        1 ggctgatcac ccaccgcgac ggaccggttc gcccggggcc gggcagttcg gtgccgacac
       61 ccgacaccga gccacccctc gatctcgagc cgcgccggac gttctaggcc gccgggcagc
      121 agcggcgccg gcggcggcat cggcggcgcg ctgctgttcg gcgcgatcgt cgggatcatg
      181 gtcctggtcg ccctctggat gcgccggacg cccccgcccg acggccagat cggcgaaacg
      241 cggacgatcg atccgagcgg ggacgggatc gcgccgcgcc ggcggtggca ccgtttcggc
      301 cggcggcccg cgccgaccac cgccgtcgcg gcctacgtcg ccctcgtcgg cgacctcgat
      361 cgccacccca acgtccgtcg cgagccggcc gagacccccg cggcccacgc cgcccggctc
      421 cgggccgatg gccttgccga gttgtccctc gatctgcttg ccgccgacta tgccctcgcc
      481 cgctatgccg gcgtgacgct gccggagcgc gaggatcggc gggccgtcgg gcgctggcgg
      541 gggctccgcc gacggctgat cacccaccgc gacggaccgg ttcgcccggg gccgggcagt
      601 tcggtgccga cacccgacac cgagccaccc ctcgatctcg agccgcgccg gacgttctag
      661 gccgccgggc ggcagcggcg ccgccggcgg catcggcgcc gccaggcgtc aggcggcgag
      721 tcgcgtcgcc aacgctcgcc gatcggccgc ccggaaccag atccagtaca ggccggtcgc
      781 gatcgaatag agggtgatga tcgtcacgaa gttgacggta tagcccgctt cgaacccgag
      841 gacggcctgg agcaccgcgt accacgttcc gccgatcacc cagccgatct gccagaggac
      901 gctcatcgcg gccgacaggc tggcccgctc ggcaggcgtc acctgctcca tcgcgaacgc
      961 ggtgaagatc gggttgccgg cgttcatcag cgaattgcgg acggccatcg cgaggatcac
     1021 cagccagatc aggggcgaga agccgaggac ggccaggaac gggatgctcg ctgcctggac
     1081 gatcacgacc gaggtgatct ggccgaaccg ccgggcaagc gtcggttggg cgaggatcgc
     1141 ggccacggtc ccgaggctcg tgaaggcgaa gacggcattc agcgaggtga ggtcgagccc
     1201 gaacttgcgc tggatgaaga tgttcaggaa cgggatgacc tggccggccc cgatcgagat
     1261 cagcaggccc gggaagagga gccgggcgaa gcgcccccgg tcgcggatca cgatcccgag
     1321 ccgagcccgc gaccgccgcg ggtccggcgg gaaggccgct ggctcgccga gtccgacgag
     1381 gcgcggcccg gcggcgaccc gcggccgatc gtcggtcagg agggcaacga tggccagcga
     1441 cgcgaccagg aggacgacca tgacgacgag gatcacgcgg taggtgcccg gtcccttcgg
     1501 atcgagaccg atgagcgacg cgatcgcggc cgcgcccacc gcgccgagca cggccgccac
     1561 gacgttcgtg acgttctgga tcgcgaactg ggcggcgaac agctcgttgc gatgctcggg
     1621 ctcgctgtgc tcggtcagga acggggcctg gacgacctgg agcgactgct ggccgacgga
     1681 ccacagggcg gcaaagacga caatcagcgc cagggcctga gtcagcagca gcccgacgag
     1741 gccgacgagg cagaccgcga tgccggcggc gatgatcgtc cgccgcccga agcgatccga
     1801 ggcggcgctg gccgggaagg ccacggcggc gccggcgatc gaggcgatcg tcgagacgac
     1861 cccgatcgtc gccgtcgaca gcccgagcga cgcgaggtac aggttgaagt cgatccagta
     1921 gaggctgagc gcagcgcctg cgacgaaggt catgacgagg aacaggcgcg cgtcgcgacg
     1981 gaagcggcgg accccccgga aggccgccca cggccccctc gattgtgtcc tcaccgggcc
     2041 cgggccccga cccggacgat caaaccgcac gcagccactc ctcgggcgcc gtcgatcggg
     2101 catgtcgaca tcggcgccga gcgaagtatg cccgtcgccc gcgagccggc cgcacccgga
     2161 acggccgcgg cgagacgcgg gcgagacgcg ggcgagacgc gggcgagacg agggcgagac
     2221 gcgggcccgc cagcattcct cgatcacgct ggaggggaca tgcggaagtc cacgtccggg
     2281 tcgtcgtcgg tgacggtcct gcgtcagatc gagacaacct cgccgtttcg cggcctcggc
     2341 cgactcccgg acgagtggcg gtcggaactg ctcgctctcg gcgacgagga cctgggtcgg
     2401 gcgatcacga tcctgctgca ccacgcggca gccctgccgt ccgcagccgc ggcgggcgcg
     2461 tccgaggcga tcgacgagct ggtcggcgga ggtcccatcg accgcctgcg gcccgggctc
     2521 gatccggctg acctcgtcgc ggtcttcgcg gccatcagtc gggacgccag ccggcaggac
     2581 ctcacccgcg tcgtcgtccg gctcctgcag ctgttcgatc ccgacgacgt cctcgcggtg
     2641 gtcggggtcg acgcgcgatc gctcgacctc cgcgcccggt tgagcggaga gttcgcccag
     2701 gcagcgatgg cgccgccacc cgacgacaaa ccgcattcac gcggcatcga cttcgaccag
     2761 ttcgggga
//
LOCUS       DATAIW010000284        12783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_246112, whole genome shotgun sequence.
ACCESSION   DATAIW010000284 DATAIW010000000
VERSION     DATAIW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246112"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(582..923)
                     /locus_tag="VHM48_08910"
     CDS             complement(582..923)
                     /locus_tag="VHM48_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HEX2755570.1"
                     /translation="MHEVSLVAELVEICEQQAAGRRVDVVRVRHATTIPPEALEQAFR
                     MLTDGGSLAGARLEAEPFDIELSCGCGFDGALGHDDLAGSVAVCPSCAAVSTLHRTAE
                     LELLEVIFAGP"
     gene            complement(916..1428)
                     /locus_tag="VHM48_08915"
     CDS             complement(916..1428)
                     /locus_tag="VHM48_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX2755571.1"
                     /translation="MRDVLVIGYGNALRTDDGLGWHAAERLAADPRLAGATVLQRHQL
                     TPELALDISRAAFVVFVDASRGPAGALSVEPVEAATDATLSWSHHVDPSVLIALSRDL
                     YSRAPDAVAVSVGVASDAAGDRLSPVVEAALPDVAATVARLVAERGGAVPATAAIVEP
                     ASTTAEHGRA"
     gene            complement(1428..2849)
                     /locus_tag="VHM48_08920"
     CDS             complement(1428..2849)
                     /locus_tag="VHM48_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX2755572.1"
                     /translation="MTSQIVIDPITRIEGHSKVTIHLDDRGEVTDAQFHVTQFRGFER
                     MVQGRPVHEMPSIMARICGICPVSHLIASSRACDEIMAVEPPPTGADLRRIMNLAQMV
                     QSNALSFFHLSSPDLLFGFDGEVASRNILGVAKANPGLARDGIGVRRFGQQIIEWLGG
                     KRIHPSWVVPGGVDAPLGAETRDRILSTIPEAIAAVERALAWYKSELTSWEEEAAVFG
                     SFRSAFMGLVDQDGNVDYYDGLLRVMDADGHLLADRIDPRTYQEYLGEAVEPWSYLKS
                     VYWKTLGYPDGVYRVGPLARVIVAAQMGTPRADLELDEFRRRLGRVPASSFHYHYARL
                     IDTLHCIEKIDELLRGEDILSPRVRSTGGINRNEGIGVSEAPRGTLIHHYKVDDDGIV
                     QWANLVIATGHNNLAMNRSVKQVAQRYVKSAALTEGMLNRVEAVIRCYDPCLSCSTHA
                     LGEMALHLQLIGPDGAILDELRR"
     gene            complement(2860..3414)
                     /locus_tag="VHM48_08925"
     CDS             complement(2860..3414)
                     /locus_tag="VHM48_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755573.1"
                     /translation="MTAGTPTKAAKPRFATVWLDGCSGCHMSFLDMDERLLEIAELVD
                     IVYSPLIDVKVYPENVDICLVEGAVSSEEDLHKIRMVRERTKTLVSFGDCAVTANVPG
                     MRNPIGVRPLLERAYIENVTLNPGIPLDVVPALLPTARPVHRVVTVDVFLPGCPPSAD
                     LIHQSLVDLLEGRTPAAHGARFGR"
     gene            complement(3411..4127)
                     /gene="hoxU"
                     /locus_tag="VHM48_08930"
     CDS             complement(3411..4127)
                     /gene="hoxU"
                     /locus_tag="VHM48_08930"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HEX2755574.1"
                     /translation="MTVHTLRIDGIDVAGTDGQSILSVATENGITIPTMCHLDGLSDT
                     GSCRLCVVEVAGSPKLTPACTTAVVEGMDITTASAQLLEYRRTITEMLFLERNHVCAV
                     CVANNHCELQNLAEDFGINHFELPVINPRVGIDASHPFFAIDHNRCIMCVRCVRVCEE
                     VEGARTWGVMGSGLESRVVTDMGTPWGQSTTCTSCGKCVQVCPTGALFAKGRSIAQGS
                     KVPRPFIPYLRVIDPPEGPP"
     gene            complement(4124..5788)
                     /locus_tag="VHM48_08935"
     CDS             complement(4124..5788)
                     /locus_tag="VHM48_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoF family protein"
                     /protein_id="HEX2755575.1"
                     /translation="MNGPLPAVDRRQAVRRDVELIAQPDGPARASAYVCEAVSCLSTK
                     SHDVLLGIGEQVAERGMKDIAVKRVGCLGLCAEGPLVQIPETGEFFRRVGLDDVGPIV
                     AALAAVGDDPARVSQQPFFARQVRIATENSGRVDPESVEDYVAVGGYAALRRALTSMT
                     PAEVREEVVRSGLRGRGGAGYSAGLKWNTVAKAAGAPKYVICNADEGDPGAFMDRNIL
                     ESDPHRVLEGMAIAAFAVGASSGYVYCRGEYPLVVSRVRKAIKDATRAGYLGARIMDT
                     EFSFELDVRLGAGAYVCGEETALIASIEGGRGSPIPRPPYPAVAGLWHRPTLINNVET
                     LANIAPIVRNGGDWFASIGTATSKGTKVFAIAGRVVNTGIVEIPMGTTLREIVFDIGG
                     GIVDGRAFKAVQTGGPAGGCIPAEFLDMPVDYESLMGVGSFMGSGGMIVMDETSCMVD
                     VAKFFMDFMREESCGKCAPCRVGTTQLWMMLDAITRGEATMVDLTRMEKLAGMVQRTS
                     LCGLGQGAPNPIFSTIRHFRDEYVAHIVDRVCPAGVCKMSATAVLA"
     gene            complement(5785..6285)
                     /locus_tag="VHM48_08940"
     CDS             complement(5785..6285)
                     /locus_tag="VHM48_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2755576.1"
                     /translation="MLVARAKPSPPSDDQRWQTVETRMRRLGNRPDALIEALHSAQQA
                     FGYIDADALGYIGETLSVPPSKVFGVATFYHYFTLKPRGAHTCVVCTGTACFINGADE
                     ILASIEARIGVKPHETTADGTLSLMTGRCFGSCSLAPAAIVDGEVEAHVDVAELVARL
                     ERLEPQ"
     gene            6555..10199
                     /gene="nifJ"
                     /locus_tag="VHM48_08945"
     CDS             6555..10199
                     /gene="nifJ"
                     /locus_tag="VHM48_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX2755577.1"
                     /translation="MDHRYECLDGNEAAARIAYAASEVISIYPITPASQMAEHCDDWS
                     AARRPNLWGAVPDVIEMQSEAGAAGALHGALQKGALGTSFTASQGLLLMIPNMFKIAG
                     ELTPTVLHVAARTVATHALSIFGDHSDVMHARTTGWAMLAAGSVQEAHDFALVAHAAT
                     LRARIPFLHFFDGFRTSHEINKIAVLETDDIRALIRDEDVLAFRRRGLTPDAPVVRGT
                     AQNPDVFFQAREACNPYHLAVPGIVEEVMAELATRTGRQYNLVDYHGAADAERVIVVM
                     GSAAGAVEETVDTLVAAGEKVGMLRIRLFNPFPAEQIVAALPSTVRAIAVLDRTKEPG
                     AIGEPLYLDIVAALSEAMDGDSPPWATTPRVIGGRYGLSSKEMTPSMVKPIFEELDAA
                     RPKRHLTVGIYDDVTHLSLPIDGGFRMARPAGEVQALFFGLGADGTVGANKASVKIIG
                     EGTDLHAQGYFVYDSKKSGSVTTSHLRFGPEPIRSTYLVDDADFVACHQFGLLGKTKI
                     LELAKHGATFLLNAPYGPDEVWDHLPAAVQRMLVDKEIDFWVIDALAVAAVAGMGSRI
                     NTVMQPCFFQLARILPPEDAIRRIKGFVEKTYAKRGEAVVARNFAAIDLSLAALGHVR
                     LGAVGEDAPVAPAANVTDFVARFTTRLMAGDGDQLPVSAMPVDGTFPTGTTRYEKRAI
                     AQMIPVWDPSICIDCGKCAMVCPHATIRMKVFPTAAVADAPAAFLSKEFKSRELADHR
                     LTIQVAPDDCTGCGVCVDVCPAKSKTDSSHKAIDMAPVAAHRDIERPNWDFFQTIAPL
                     DRSLLPHDTVKGSQVLEPLFEFSGACGGCGETPYLRLVSQLFGDRMIVANATGCSSIY
                     GANLPTTPWTVNGAGRGPAWANSLFEDNAEFGLGMRLALDAQTDHARQLVLRLAPALG
                     DEIVQGLLGADQEDEAGIDRQRERVDRLRELLTAIEGPLAADAQHLLALAGDLVRKGV
                     WIIGGDGWAYDIGFGGVDQVLSSGRNVNILVLDTEVYSNTGGQSSKATPRGAVAKFAA
                     AGKSTAKKDLGAIARSYGNVFVAQVSMGANDAQTTKALLEADAWPGPSLVIAYSTCIA
                     HGIDMSKSMSHQKDAVRSGYWPLYRFHPSEIDGGKPFKLDSAKPTIPIADFVATETRF
                     AVLQRTDPERAAELATLAQADADGRWRYYEQLAGMERTVPHLVHLDPSDPVVAATPNG
                     AAKQEDEGEE"
     gene            10196..11221
                     /locus_tag="VHM48_08950"
     CDS             10196..11221
                     /locus_tag="VHM48_08950"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX2755578.1"
                     /translation="MTVDLTTRYLGLELRSPLIASAAPQNSDPGHARRLDGAGIGAIV
                     LPSLFEEEIEAEEVELNRSLEQGTEAFAEALDYFPAVPSFAGAADRYLTSIERIKSAV
                     SVPVIASLNAQTAGGWVRHARRIQDAGADALELNLYHVAADADRTAAGMEAADEALIA
                     AVRAAVTIPLAVKLSPFYTAFAHFAAGAVTAGADGLVLFNRFYQPDLDLESLDVVPRL
                     DLSQAWELRLPVRWIATLRPQLGPTVSLAATSGIQSGTDALKALLVGADVAMMTSAIL
                     RNGPYHVAVVEAELRAWMTDREYESVDQLRGSASAATAANPSAFERANYMSTIRSWVA
                     PQELTPL"
     gene            complement(11495..12271)
                     /locus_tag="VHM48_08955"
     CDS             complement(11495..12271)
                     /locus_tag="VHM48_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2755579.1"
                     /translation="MAAIFRALGDPSRRLLLDRLFDRDGQTLGELAAQLPGMTRFGVM
                     RHLDVLEAAGLIETRRVGREKHHYLNPVPIRLVADRWIGKFAEPVVGRMAALKHHLEA
                     PAMLAPDHVYSVLIRATPDRIWRAITDGVETERYYYGTRVDSDWSVGGRIVYTYPDGS
                     LAADGEVIEIEPGRRVLMSFHPRWSPEIEAEGPVRMSWQIEPAGDSVSKLTVVTSGAV
                     EGGVIQTDFAGGVEYIVSGLKTLVETGEPLMPVGAGSAAG"
     gene            12477..>12783
                     /locus_tag="VHM48_08960"
     CDS             12477..>12783
                     /locus_tag="VHM48_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755580.1"
                     /translation="MLLIAVAAGCSLLPVASPSSPGQAAREALVTDYLGALERRDAAA
                     IEAMVSPAVDASADIAQVLEQYGGVRLHELRATWLDEFGGSYVVATATGTGEDGAAYE
                     "
BASE COUNT         1946 a         4564 c         4371 g         1902 t
ORIGIN      
        1 gtagccgccg gagatgagct tggcctcgcc ctctcgctcc gagaggatct tcagagcgcc
       61 gtcgaggtcc gctggccgga cgtaatcgaa aggtgccgga atcatgcgcg accgtcctcc
      121 tccgccccgg cgtcggggcg agccatggcc cgtcagtgcg aggcgacggg ctgaattcgc
      181 gacgatcggg ccggcggaga gatcggcggc ggcgtccttg ccgtgagctc acccgggggt
      241 ccgctgctcc gaagtgtgat cgctgcccgg gtgcgggtca accggccctg agcgcggccg
      301 ggtacgggcc gtggcgccgg gtcctggcgg ccggccgtcg cgccgcgccc cgcccgcacc
      361 ttgccagatt gccaccggtg gaacgttggg cgcgttccgt cgaccccggc gaccacgaat
      421 tgccccggag cgccctgcgc tgagcgtgga acgggccatt ccgcggccct cccggtcctc
      481 gtcgcgactc cgagggccgt cgcacctgac ctaacgttcc ggggatggca atttggccgg
      541 atcggcgcct tgccggggcg cccgtcgcgc gacgcacccg gtcacggccc ggcgaagatc
      601 acctcgagca gctcgagctc ggcggtccga tggagtgtcg agaccgcggc gcacgacggg
      661 cagacggcca cggaccccgc cagatcgtcg tgaccgagcg ccccgtcgaa cccgcagccg
      721 cacgaaagct cgatgtcgaa cggctcggcc tcgaggcgcg cgccggccag gctgccgccg
      781 tcggtcagca tccggaatgc ctgctcgagc gcctctggcg gaatcgtggt cgcgtgccgg
      841 acgcggacca cgtcgacccg ccggccggcc gcctgctgct cgcagatctc gaccagctcg
      901 gccacgaggg acacctcatg cacggccgtg ctccgcggtc gtcgacgccg gctccacgat
      961 cgcggccgtc gccggcaccg ccccgccgcg ctcggcgacc agcctggcga ccgtggccgc
     1021 gacgtccggc agcgcggcct cgacgacggg cgacaggcga tcccccgccg catccgaggc
     1081 cacgccaacg ctgacggcga cggcatccgg cgcccgactg tacaggtcgc gggacagggc
     1141 gatcaacacc gacggatcga cgtgatggga ccacgacagc gtggcgtccg tcgccgcctc
     1201 caccggctcg accgacagag ctccggcggg accgcggctg gcgtcgacga agacgacgaa
     1261 ggcggcgcgg ctgatgtcga gggccagctc cggggtcagc tggtggcgct ggaggaccgt
     1321 cgcgccggcc agacgcgggt cggcggcgag gcgctcggcg gcgtgccagc caaggccgtc
     1381 gtcggtccgg agcgcgttgc catagccgat gacgaggacg tcccgcatca ccgccgcagc
     1441 tcgtcgagga tcgccccgtc cggcccgatg agctggaggt ggagggccat ctcgccgagg
     1501 gcatgggtcg agcaggacag gcacgggtcg tagcagcgga tgaccgcctc gacgcggttg
     1561 agcatcccct cggtcagggc tgcgctcttg acgtagcgct gggcgacctg cttgacgctg
     1621 cggttcatcg cgaggttgtt gtggccggtc gcgatcacga ggttcgccca ctggacgatc
     1681 ccgtcgtcgt cgaccttgta gtgatggatc agcgtcccac gcggcgcctc ggagacgccg
     1741 atcccctcgt tccggttgat gccgcccgtc gaccgcaccc gcggcgacag gatgtcctcg
     1801 ccacggagca gctcgtcgat cttctcgatg cagtggaggg tgtcgatcag tcgggcgtag
     1861 tggtagtgga aggagctggc gggcacccgg ccgagccggc gacggaactc gtcgagctcg
     1921 aggtcggccc gcggcgtgcc catctgggcg gcgacgatca cgcgggcgag cggccccacc
     1981 cggtagaccc cgtcgggata gccgagcgtc ttccagtaga ccgacttcag gtaggaccac
     2041 ggctcgacgg cctcgccgag gtactcctgg tacgttcgcg ggtcgatccg atcggcgagg
     2101 aggtggccgt cggcatccat gacccggagg agcccgtcgt aatagtcgac gttgccgtcc
     2161 tggtcgacga ggcccatgaa ggcggatcgg aacgacccga agacggccgc ctcctcctcc
     2221 cacgaggtga gctcggactt gtaccaggcg agggcccgct cgaccgcggc gatcgcctcc
     2281 gggatcgtcg agaggatccg gtcgcgcgtc tcggctccga gcggggcatc gacgccgccc
     2341 ggcacgaccc aggacgggtg gatccgcttg ccgccgagcc actcgatgat ctgctggccg
     2401 aagcgccgga cgccgatgcc gtcgcgggcc aggccggggt tcgccttggc aaccccgagg
     2461 atgttccggc tggccacctc gccatcgaac ccgaagagca ggtcgggcga cgagaggtgg
     2521 aagaagctca gggcgttcga ctggaccatc tgggcgaggt tcatgatccg gcgcaggtcc
     2581 gctccggtcg gtggcggctc gaccgccatg atctcgtcgc aggccctcga cgaggcgatc
     2641 aggtggctga ccgggcagat cccgcagatc cgggccatga tcgatggcat ctcgtggacc
     2701 ggccggccct ggaccatccg ttcgaaccca cggaactggg tcacgtggaa ctgggcgtcg
     2761 gtcacctcgc cacggtcgtc gaggtggatc gtgaccttcg agtggccctc gatgcgggtg
     2821 atcggatcaa tgacgatctg gctggtcacg cgtgagtcct caacggccga aacgggcgcc
     2881 gtgcgctgcg ggggtgcgtc cctcgagcag gtccacgagc gactggtgga tgagatcggc
     2941 cgacggcggg caacccggca ggaagacgtc cacggtcacg acgcggtgga cgggtcgggc
     3001 ggtcggcagc agggccggca cgacatcgag ggggatccca gggttgagcg tcacgttctc
     3061 gatgtaggcc cgctcgagga gcggccggac gccgatcggg ttgcgcatgc ccgggacgtt
     3121 ggccgtgacg gcgcagtcgc cgaacgagac gagcgtcttc gtccgctccc ggaccatcct
     3181 gatcttgtgg aggtcctcct cgctcgagac ggcgccctcg accaggcaga tgtcgacgtt
     3241 ctcggggtag accttcacgt cgatcagcgg gctgtacacg atgtcgacga gctcggcgat
     3301 ctcgagcagc cgctcgtcca tgtccaggaa cgacatgtgg cagcccgagc agccatccag
     3361 ccagaccgtg gcgaaccgcg gcttggcggc cttggtcggc gtcccggccg tcatggcgga
     3421 ccttccggcg ggtcgatgac gcgaaggtac gggatgaacg ggcgggggac cttgctgccc
     3481 tgggcgatcg agcggccctt cgcgaacagg gcgccggtcg ggcacacctg gacgcacttg
     3541 ccgcagctcg tgcaggtcgt cgactggccc cacggcgtgc ccatgtcggt cacgacccgg
     3601 gactcgagcc cggagcccat cacgccccat gtccgggcgc cctcgacctc ctcgcagacc
     3661 cggacgcacc ggacgcacat gatgcagcgg ttgtggtcga tcgcgaagaa cgggtgactg
     3721 gcgtcgatgc cgaccctggg gttgatgacc ggcagctcga agtggttgat gccgaagtcc
     3781 tcggcgaggt tctgcagctc gcagtggttg ttggccacgc acactgcgca gacgtggttg
     3841 cgctcgagga acagcatctc cgtgatcgtc cgccggtact cgaggagctg cgccgacgcg
     3901 gtcgtgatgt ccatgccctc gacgaccgcc gtcgtgcagg cgggcgtgag cttgggcgat
     3961 ccggcgacct cgacgacgca cagccggcac gagccggtgt cggacaggcc gtcgagatgg
     4021 cacatcgtcg ggatcgtgat cccgttctcg gtcgcgaccg acaggatcga ctggccgtcg
     4081 gtgccggcga cgtcgatgcc gtcgatccgg agcgtgtgaa cggtcacgcg agcaccgccg
     4141 ttgcggacat cttgcacacc cctgccggac agacccggtc gacgatgtgg gcgacgtact
     4201 cgtcacggaa gtggcgaatc gtgctgaaga tcgggttcgg ggcgccctgg ccgaggccgc
     4261 acaggctggt ccgctgaacc atgccggcca gcttctccat gcgtgtgagg tcgaccatcg
     4321 tcgcctcgcc ccgcgtgatc gcgtcgagca tcatccagag ctgcgtcgtc ccgacccggc
     4381 agggggcgca cttgccgcac gactcctcgc gcatgaagtc catgaagaac ttcgcgacgt
     4441 cgaccatgca actcgtctcg tccatcacga tcatgccgcc ggagcccatg aacgagccga
     4501 cgcccatgag cgattcgtag tcgaccggca tgtcgaggaa ctcggccggg atgcagccgc
     4561 cggccggtcc gccggtctgg acggccttga acgcccggcc gtcgacgatc ccgccgccga
     4621 tgtcgaagac gatctcgcgc agggtcgtgc ccatcgggat ctcgacgatg ccggtgttga
     4681 cgacccggcc ggcgatcgcg aagaccttcg tgcccttgct cgtcgccgtc ccgatcgagg
     4741 cgaaccagtc gccgccattg cggacgatcg gcgcgatgtt ggcgagcgtc tcgacgttgt
     4801 tgatcagggt cgggcggtgc cacagaccgg caacggcggg gtatggaggc cgcgggatcg
     4861 gcgaaccgcg tccgccttcg atcgaggcga tgagggcggt ctcctcgccg cacacatagg
     4921 caccggcgcc gagccggacg tcgagctcga acgagaactc ggtatccatg atcctggccc
     4981 cgaggtaccc ggcccgggtg gcatccttga tggccttgcg gacccgcgag accacgaggg
     5041 gatactcgcc gcggcagtac acgtaaccgc tgctcgcccc gaccgcgaac gcggcgatcg
     5101 ccatcccctc gaggacccgg tgcgggtcgc tctcgaggat gttccgatcc atgaacgccc
     5161 ccgggtcgcc ttcatccgcg ttgcagatga cgtacttcgg ggcgccggcc gccttggcga
     5221 ccgtgttcca cttgaggccc gccgagtatc cggcaccgcc gcggccgcgc aacccgctcc
     5281 ggacgacctc ctcgcggacc tccgccgggg tcatcgaggt gagcgcgcgc cggagcgccg
     5341 catagccgcc cacggcgacg taatcctcga cgctttccgg atcgacccgc ccggagttct
     5401 ccgtggcgat gcggacctgg cgggcgaaga acggctgctg ggagacccgc gccgggtcgt
     5461 cgccgacggc ggccagggcc gcgacgatcg gtccgacgtc atcgagcccg acccggcgga
     5521 agaactcgcc ggtttcgggg atctggacga gcgggccctc cgcgcacagg ccgaggcaac
     5581 cgacgcgctt gaccgcgatg tccttcatgc cgcgttccgc cacctgctca ccgatcccca
     5641 gcaggacgtc gtgcgacttc gtcgacaggc agctcacggc ctcgcacacg tacgcggagg
     5701 cgcgggcagg tccgtcgggc tgggcgatga gttcgacatc gcggcgcacc gcctggcgcc
     5761 ggtcgaccgc cggcagcggg ccgttcactg cggttccagc cgttccagcc gggcgacgag
     5821 ctcggccacg tccacgtgtg cctcgacctc gccgtcgacg atcgccgccg gcgcgaggct
     5881 gcacgagccg aaacaacgac cggtcatcag cgagagcgtg ccgtccgcgg tcgtttcgtg
     5941 gggcttcact ccgatcctcg cctcgatcga ggcgaggatc tcgtcggcgc cgttgatgaa
     6001 gcacgcggtg ccggtgcaga cgacgcaggt gtgggcgccg cgcggcttga gcgtgaagta
     6061 gtggtagaag gtcgcgaccc cgaagacctt ggacggcggc accgacagcg tctcgccgat
     6121 gtagccaagg gcgtcggcgt cgatgtagcc gaaggcctgc tgggcagaat ggagggcctc
     6181 gatcaacgcg tccgggcggt tcccgagccg acgcatccgg gtctcgacgg tctgccagcg
     6241 ctggtcgtcg ctgggtggcg acggctttgc gcgcgctacg agcacttgtt gaacgtctcc
     6301 ctgagtgggt cgcagcgccg ccgttcaacg tccccctggc ggcgcctgtc gtccagaccc
     6361 tatcaccgcc cgatgtgcgt cgtcacgggt ggttgggccc tccccggggg cgccgaatgg
     6421 ccccccaaac gacgccactc ggcctcgccg ccgccgtctc ggacgggcgc ataatcgtgg
     6481 ctccggggtc cgcgcacgac cggcgccgag tcgcgaccag gccctcaagc tcacggagag
     6541 gagcctgtcg aaccgtggac catcgctacg agtgccttga cggcaacgaa gccgccgccc
     6601 ggatcgccta cgcggcgtcc gaggtgatct cgatctaccc gatcaccccg gcttcgcaga
     6661 tggccgagca ctgcgacgac tggtcggccg ctcgccggcc gaacctgtgg ggcgccgtcc
     6721 cggacgtcat cgagatgcag tccgaggcag gagccgcggg cgccctccac ggggctctcc
     6781 agaagggtgc cctcgggacg tcgttcacgg cctcgcaggg cctccttctg atgatcccga
     6841 acatgttcaa gatcgccggc gagctgactc cgacggtcct tcatgtcgcg gcccggacgg
     6901 tggccaccca cgccctctcg atcttcggcg accacagcga cgtcatgcac gcccggacga
     6961 ccggctgggc gatgctcgcg gcaggttcgg tccaggaggc gcacgacttc gccctggtgg
     7021 cccacgccgc gaccctccgc gcccggatcc cgttccttca cttcttcgac ggcttccgga
     7081 cgagccacga gatcaacaag atcgcggtcc tcgaaaccga cgacatccgg gccctgatcc
     7141 gcgacgagga cgtcctcgcc ttccgccgcc gcggcctcac gccggatgcc ccggtcgttc
     7201 gcggcacggc tcagaacccc gatgtcttct tccaggcccg cgaggcctgc aacccgtacc
     7261 acctcgcggt tcccgggatc gtcgaagagg tcatggcgga gctcgccacg cgaaccggcc
     7321 gccagtacaa cctcgtcgac tatcacgggg cggcggacgc cgagcgcgtg atcgtcgtga
     7381 tgggctcggc cgccggcgcc gtcgaggaga cggtcgacac gctcgtcgcg gccggcgaga
     7441 aggtcggcat gctccggatc cgcctcttca acccgttccc cgctgagcag atcgtcgccg
     7501 ccctgccgtc gacggtccgg gccatcgccg tcctcgatcg gaccaaggaa ccgggtgcga
     7561 tcggcgagcc gctctacctc gacatcgtcg ccgcattgag cgaggcgatg gatggcgaca
     7621 gcccgccctg ggcgaccacg ccccgggtca tcggcggccg ctacggcctc tcctcgaagg
     7681 agatgacgcc atcgatggtc aagccgatct tcgaggagct ggacgcggcc cgcccgaagc
     7741 gccacctcac ggtcggaatc tacgacgacg tgacccacct cagcctgccg attgacggcg
     7801 gtttccggat ggcccgcccg gccggcgagg tccaggcgct cttcttcggc ctcggcgccg
     7861 acggcacggt cggcgccaac aaggcgtcgg tcaagatcat cggcgaaggg accgacctgc
     7921 acgcccaggg ctacttcgtc tacgactcga agaagtcggg ctcggtcacg acgtcccacc
     7981 tccggttcgg ccccgagccg atccgctcga cctatctcgt tgacgacgcc gacttcgtgg
     8041 cctgccacca gttcggcctg ctcggcaaga cgaagatcct cgagctcgcc aagcacggcg
     8101 cgacgttcct cctcaacgct ccctacggac ccgacgaggt ctgggaccac ctgcccgccg
     8161 ccgtccagcg gatgctggtc gacaaggaga tcgacttctg ggtgatcgac gcgctcgcgg
     8221 tcgccgccgt ggccgggatg ggcagccgga tcaacacggt catgcagccg tgcttcttcc
     8281 agctcgcccg gatccttccg cccgaggacg cgatccgccg gatcaagggc ttcgtcgaga
     8341 agacctatgc caagcgcggc gaggcggtcg tcgcccgcaa cttcgcggcg atcgatctct
     8401 cgctcgccgc cctcggccac gtccgactcg gggccgtcgg cgaagacgcg cccgtcgcgc
     8461 cagccgccaa cgtcacggac ttcgtcgccc ggttcacgac ccgcctgatg gccggcgacg
     8521 gcgaccagct gccggtcagc gcgatgccgg tcgacggaac gttcccgacc ggcacgacca
     8581 ggtacgagaa gcgggcgatc gcccagatga tcccggtctg ggatccgtcg atctgcatcg
     8641 actgcggcaa gtgcgcgatg gtctgcccgc atgccacgat ccggatgaag gtcttcccga
     8701 ccgcggccgt tgccgacgcc ccggcggcgt tcctcagcaa ggagttcaag tcgcgcgagc
     8761 tggcggatca tcgcctcacg atccaggtcg cgccggacga ttgcaccggc tgcggcgtat
     8821 gcgtcgacgt ctgccccgcg aagagcaaga ccgactcgag ccacaaggca atcgacatgg
     8881 cgccggtcgc cgcccatcgc gacatcgagc gaccgaactg ggacttcttc cagacgatcg
     8941 cgccgctcga ccggagcctc ctgccccacg acacggtcaa gggcagccag gtcctcgagc
     9001 cgctgttcga gttctccggg gcctgcggcg gctgcggcga gacgccctac ctccgcctcg
     9061 tcagccagct cttcggcgac cggatgatcg tggccaacgc caccggctgc tcctcgatct
     9121 acggcgccaa cctgccgacc acgccgtgga ccgtcaacgg cgcgggccgt ggcccggcct
     9181 gggccaactc cctgttcgag gacaacgccg agttcggtct cggcatgcgc ctcgcccttg
     9241 acgcccagac cgatcacgcc cgccagctcg tcctgcgtct cgccccggcc ctcggcgacg
     9301 agatcgtcca gggcctcctc ggcgccgacc aggaggacga ggccgggatc gaccgccagc
     9361 gcgagcgggt cgaccgcctg cgcgagctgc tgaccgcgat cgagggcccg ctcgcggcgg
     9421 acgcccagca ccttctcgcc ctcgccggcg acctcgtccg gaagggcgtc tggatcatcg
     9481 gcggcgacgg ctgggcctac gacatcggct tcggcggggt cgaccaggtc ctttcgtccg
     9541 gccggaacgt caacatcctc gtcctcgaca cggaggtcta ctccaacacc ggcggccagt
     9601 cctcgaaggc gaccccgcgc ggcgcggtcg ccaagttcgc cgccgccggc aagagcacgg
     9661 ccaagaagga cctcggggcc atcgcccgct cgtacggcaa cgtcttcgtc gcccaggtgt
     9721 cgatgggcgc gaacgacgcc cagacgacga aagccctgct cgaggccgat gcctggccgg
     9781 gcccgtcgct cgtcatcgcc tacagcacct gcatcgccca cggcatcgac atgagcaagt
     9841 cgatgagcca ccagaaggac gcggtccgga gtggctactg gccgctctat cgcttccacc
     9901 cctcggagat cgacgggggc aagccgttca agctcgactc ggccaagccg acgatcccga
     9961 tcgccgactt cgtcgcgacc gagacgcgct tcgccgtcct ccagcggacc gaccccgagc
    10021 gcgccgcgga gctcgcgacg ctggcccagg ccgacgccga cggacgctgg cgctactacg
    10081 agcagctcgc ggggatggag cgaaccgtcc cccacctcgt ccatctcgac ccgtcggatc
    10141 cagtggtcgc agccacgccg aacggcgcag ccaagcagga agacgaaggc gaggaatgac
    10201 cgtcgacctc accacccgct atctcgggct cgagctccgt tccccgctca tcgcctcggc
    10261 ggcgccgcag aacagcgacc cgggccacgc ccgccggctg gacggtgcag ggatcggcgc
    10321 gatcgtcctg ccgtcgctgt tcgaagagga gatcgaggcc gaggaggtcg agctcaaccg
    10381 ctcgctcgag cagggaaccg aggccttcgc cgaagccctc gactacttcc ccgccgtccc
    10441 atcgttcgcc ggtgccgccg atcggtacct gacctcgatc gaacggatca agtcggcggt
    10501 ctcggtcccg gtcatcgcca gcctcaacgc ccagaccgcc ggaggatggg tccgccacgc
    10561 acgacggatc caggacgccg gcgccgacgc gctggagctg aacctctatc acgtcgcagc
    10621 cgacgccgat cggaccgccg ccgggatgga ggccgccgac gaggccctga tcgccgccgt
    10681 ccgggccgcg gtcacgatcc cgctggccgt caagctcagc ccgttctaca ccgcgttcgc
    10741 ccacttcgcg gcgggggcgg tgaccgccgg cgccgatggc ctcgtcctgt tcaaccgctt
    10801 ctaccagccc gatctcgacc tcgagagcct cgacgtcgtg ccgcgcctcg acctgagcca
    10861 ggcgtgggag cttcgcctcc cggtgcgctg gatcgcgacc ctccgacccc agctcgggcc
    10921 gacggtctca ctggcggcga cgtccgggat ccagtcgggc acggacgccc tgaaggccct
    10981 cctcgtcggc gccgatgtgg cgatgatgac gtcggccatc ctgcgcaacg gcccgtacca
    11041 cgtcgcggtg gtcgaggcgg agctgcgggc gtggatgacc gatcgcgagt acgagtcggt
    11101 cgaccagctc cgcggcagcg ccagcgcggc gaccgcggcg aacccgtcgg cattcgagcg
    11161 ggcgaactac atgtcgacga tccgctcgtg ggtcgcgccg caggaactta cgccgctctg
    11221 agttcggggc gccgcggcgg ggcggcgcgt tcagtggatt cgaccgattt gcccatacga
    11281 cgccccggat ggcgctggac cggcgatacc cgcctggcgg cgcggatccc gttcgattcg
    11341 ttcccatacg tctgcccggg agcgcgtatg actcggattc gctcctgcgc gggcctctcg
    11401 aaccgccgaa tgccatccac ggcgacgtat gggaagaatc cggcgacggc cgaggtagac
    11461 gtggtgtccg cctcgacgtc gcgcgaggtg ccggtcagcc ggccgcggac ccggcgccga
    11521 ccggcatcag cggctcgccc gtctcgacga gcgtcttcag gcccgacacg atgtactcga
    11581 ccccgcccgc gaagtcggtc tggatgacgc cgccctcgac cgcgccgctc gtgacgaccg
    11641 tcagcttcga gacgctgtcg ccggccggtt cgatctgcca gctcatccgg accgggccct
    11701 cggcctcgat ctcggggctc cagcgcgggt ggaagctcat caggacccgc cgcccgggtt
    11761 cgatctcgat cacctcgccg tcggcggcca gtgatccatc cgggtaggtg tagacgatcc
    11821 ggccgccgac cgaccagtcg gagtcgacgc gcgtgccgta gtagtagcgc tcggtctcga
    11881 cgccgtcggt gatcgcccgc cagatgcggt cgggcgtcgc ccggatcagg accgaataga
    11941 cgtgatcggg tgcgagcatc gcgggtgcct ccaggtggtg cttcagcgcg gccatccgcc
    12001 ccacgaccgg ctcggcgaac ttgccgatcc agcggtccgc gacgaggcgg atcgggacgg
    12061 ggttgaggta gtggtgcttc tcgcggccga cccgccgggt ctcgatcagg ccggcggcct
    12121 cgagcacgtc gagatgacgc atgacgccga accgggtcat cccagggagc tgagccgcaa
    12181 gctcgccgag cgtctgtccg tcgcgatcga acagccggtc gaggagcaac cgccggctcg
    12241 ggtcgccgag agcccggaag atcgccgcca tgccgtccac gaccgcatta tgtgacttgc
    12301 tagtcacatg tcaaggggtg atcaggcccc tgtgtgttat aggcgtttgg tgggtgggtg
    12361 tcacacggcc cagtcgccaa acgcctgact ggttgccaca ccacgacacc acaggtaggt
    12421 tcggctcaat ggacacaaca ggacgaatgc cgttgatccg atccgcgctt gcgacggtgc
    12481 tgctcatcgc ggtcgcggcg ggctgctcgc ttctgccggt ggcatcgccc tcgtcccctg
    12541 gacaagctgc ccgtgaggcg ctggtcacgg actacctcgg cgcgcttgag cgtcgtgatg
    12601 ccgccgccat cgaagcgatg gtcagccccg cggtcgatgc gagcgccgac atcgcacagg
    12661 tcctggagca gtatggcggt gtcaggctgc acgagcttcg agcgacgtgg ctcgacgagt
    12721 tcggcggcag ctacgtcgtg gccacggcca ccgggaccgg ggaggatggc gctgcctacg
    12781 aga
//
LOCUS       DATAIW010000285         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_126918, whole genome shotgun sequence.
ACCESSION   DATAIW010000285 DATAIW010000000
VERSION     DATAIW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126918"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VHM48_08965"
     CDS             complement(<1..768)
                     /locus_tag="VHM48_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2755581.1"
                     /translation="MTMKSVDPAHLRSVVLTGHAGGGKTTLAEQLLFRAGAVQRLGRV
                     DDGTAHLDFEPEEQKRKESLSLAVATFEHEATRITLVDTPGYPDFVAEVISGFAAADG
                     ALFVVDASTDIGAGLEAAVALGRSSGRAACFFLNKCDRENGDASATLDRLRERFGNKI
                     APLHLAIGKAESFSGYVDLVHRKAWSFEGGKEVEIPVPAELAGEIAARRDQLLEAAAE
                     ADDEVMTKYLDGVEISDAELDTCLHRGVRDSILAPVLV"
     gene            884..1639
                     /locus_tag="VHM48_08970"
     CDS             884..1639
                     /locus_tag="VHM48_08970"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2755582.1"
                     /translation="MSGHSKWSTIKRAKGVTDAKRSAVFTKVAREISVAARQGGGDPD
                     ANYRLRLAMEKARSVNMPADNIKRTIDKATGGGDADQFEEIVYEGYGPGGVAILVEAA
                     TDNRNRTAAEVRSMFTKAGGQLAGSGAVAWQFEPRGLIAVARDGKLDADEVTLAAIDA
                     GAEDVDTELDETIEIYTAPGDLEAVRKALEGAGVPVDSAENTMVAKQTVELDSHKARQ
                     ALRLVELLEDLEDVQRVTANFDIPEDVYAEVAG"
     gene            1698..2252
                     /gene="ruvC"
                     /locus_tag="VHM48_08975"
     CDS             1698..2252
                     /gene="ruvC"
                     /locus_tag="VHM48_08975"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX2755583.1"
                     /translation="MIILGIDPGTAALGWGLVDQTAGRLRAVDYGCLVTSPDLPLGER
                     LLAIHRHVTELIALHDPAVVAVERVFFSRNAQTAIAVGHARGVILLAAAEAGRPVREA
                     TPNEVKSAIAGYGAADKQQVQRMVQIVLGMASLPTPDDAADALAIAVCIANGMGQTGR
                     SSRTTAGVLDRSSINPIVRGSTPY"
BASE COUNT          405 a          920 c          956 g          366 t
ORIGIN      
        1 gacgaggacc ggggcgagga tcgagtcgcg gacgccgcga tggaggcagg tgtcgagctc
       61 ggcgtcgctg atctcgacac cgtcgaggta cttggtcatg acctcgtcgt ccgcctcggc
      121 cgccgcttcg agcagctggt cgcgacgggc ggcgatctcg ccggcgagct cggccggcac
      181 ggggatctcg acctccttgc ccccttcgaa ggaccaggcc ttgcggtgga cgagatcgac
      241 gtagccgctg aacgactcgg ccttgccgat cgccaggtgg agcggcgcga tcttgttgcc
      301 gaatcgctcg cgcaggcgat cgagcgtcgc cgacgcgtcg ccgttctcgc ggtcgcactt
      361 gttgaggaag aagcaggcgg cccggccgct cgatcggccg agcgcgaccg cggcctccag
      421 cccggcgccg atgtcggtcg aggcgtcgac gacgaacagc gcgccgtcgg cggcagcgaa
      481 gccggagatc acctcggcga cgaagtccgg gtagccgggg gtgtcgacga gcgtgatccg
      541 ggtcgcctcg tgctcgaacg tggcgaccgc gaggctcagc gattccttgc gcttctgctc
      601 ttcgggctcg aagtcgagat gggccgtccc atcgtcgacc cgacccagtc gctggaccgc
      661 gccggcacga aagaggagct gttcggcaag cgtcgtcttg ccgccgccgg cgtggccggt
      721 caggacgacg ctgcgaaggt gggcgggatc gacgctcttc atcgtcatcg gcgggaggac
      781 ctcacgactt cagacggaac cgacaattcg gggcacggaa gcgggcggga caggcccctc
      841 cgggcgggtc cggccatcat agtcggctat actccggccc gcgatgtccg gtcattccaa
      901 gtggtccacg atcaagcggg cgaagggcgt aacggacgcc aagcgcagcg ccgtgttcac
      961 gaaggtcgcc cgcgagatca gcgtcgcggc ccgccagggc gggggcgatc cggacgccaa
     1021 ctaccgcctc cgcctggcga tggagaaggc ccgctcggtc aacatgccgg cggacaacat
     1081 caagcggacg atcgacaagg cgaccggcgg cggcgatgcc gaccagttcg aggagatcgt
     1141 ctacgagggc tatggccccg gcggcgtggc gatcctcgtc gaggcggcga ccgacaatcg
     1201 caaccggacc gcggccgagg tccgttcgat gttcaccaag gccggcggcc agctcgcggg
     1261 cagtggcgcg gtggcctggc agttcgagcc gcgcggcctg atcgcggtcg cccgcgacgg
     1321 caagctcgac gccgacgagg tcaccctcgc cgcgatcgac gccggcgccg aggacgtcga
     1381 cactgaattg gacgagacga tcgagatcta caccgcgccc ggtgatctcg aggcggtccg
     1441 caaggcgctc gaaggtgccg gcgtgccggt cgactcggcc gagaacacga tggtcgccaa
     1501 gcagaccgtc gagctcgatt cgcacaaggc gcgccaggcc ctccggctcg tcgagctgct
     1561 cgaggacctc gaggacgtcc agcgggtgac ggccaacttc gacatccccg aggacgtcta
     1621 cgccgaggtc gccgggtgac gtcgggcgcc ctgcccgtcg ggttgccggt ccatcgggca
     1681 cctgcgatcg cccgccggtg atcatcctcg gcatcgaccc cgggaccgcg gccctcggct
     1741 ggggcctcgt cgaccagacg gcgggccggc tgcgggccgt cgattacggc tgcctcgtca
     1801 cctcgccgga tctccccctc ggcgagcgcc tgctcgcgat ccatcgccac gtgaccgagc
     1861 tgatcgccct ccacgatccg gcggtggtcg cggtcgagcg ggtcttcttc tcgcggaatg
     1921 cccagacggc gatcgccgtc ggccacgcgc gcggggtgat cctgctggca gcggcggagg
     1981 ccggccggcc ggtccgcgag gcgaccccca acgaggtcaa gagcgcgatc gccggctacg
     2041 gcgcggccga caagcagcag gtccagcgga tggtccagat cgtgctcggg atggcgtcgc
     2101 tgccgacgcc cgacgacgcg gccgacgccc tcgcgatcgc ggtctgcatc gccaacggga
     2161 tgggccagac gggccgttcg tcgcggacga ccgccggcgt gctcgaccgc tcgtcgatca
     2221 acccgatcgt gcgcggctcg acgccgtact agcgggcggt gcgggaggcc ctggcgcgcg
     2281 agggcggcgg gcgggcgacg cgtcgaagcc gcgaaccggc gcggtgacgc tcgcgcgcgg
     2341 ccgaaatcgc catacgccag ccgggatgcc attagaccgg cgggaccgga cgaatgttga
     2401 ctcgattagc ctctaccggc taacgggacc cgccggcagc acgattccga ccgccggcga
     2461 cgagctaacg ccaccgtcag cggcgtatga caatcgcgag tcgtcacgcg gagacccagc
     2521 gtcatgatcg cctcggttga gggcaaggtc ggcgcgatcg cgttcgactc gctcgtcatc
     2581 gaggtcggcg gcatcggcta ccgcgtcttc gccgcgccgg ccattctcgc gaccgcccag
     2641 cccgggt
//
LOCUS       DATAIW010000286         5627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_107459, whole genome shotgun sequence.
ACCESSION   DATAIW010000286 DATAIW010000000
VERSION     DATAIW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107459"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1133)
                     /locus_tag="VHM48_08980"
     CDS             complement(72..1133)
                     /locus_tag="VHM48_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2755584.1"
                     /translation="MPPRPMLPPDDLYARLELPIDASYEVIEIAWRALLKQHHPDIAG
                     PTADELAKRINVAHDWLSDPELRARYDRERHPRRVAATSFGGRRPPPPSVRSSAPDRP
                     VRRPAVVRRPVNPAQAIARHIDRIRRLTEDELDRLSLAETAPIAFIATISRFLPADRQ
                     DALRTLEPMVQDALPPRGRWDPATRDAVVGFAQDIVLEPFLDEHLSGDFRERVRERLT
                     RGWQAAVDQPRYGPNTAAVADALRRLAALAPAERARIGGLAPRGSVPAELPWPRGLTP
                     TDDDGLRVSSALARRDALALLPETTPSLARRSLGRAIHGLVLRHAFKPAELERLAGPW
                     APLVLPVPASGPRARRRPG"
     gene            complement(1186..2889)
                     /locus_tag="VHM48_08985"
     CDS             complement(1186..2889)
                     /locus_tag="VHM48_08985"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2755585.1"
                     /translation="MRLSHLFFTTLRDDPADVEMPSHRLLLRAGYIRPLGSGIYSLLP
                     LGKRVNDRVEQVIREEQDRIGGQEMEMPVVHPADIWRASGRYDAIGQELTRFKDRNGR
                     DMVLAMTHEEVVGILLADIVKSYRQLPMMVYHFQTKWRDEPRARAGLIRVREFVMKDA
                     YSCDRDEAGLDVSYQAQYDAYVRTFERLGLKTVAVSSDVGIMGGSGAHEFMVLNPAGE
                     DVLVLCEACGYAANRQVARIEHPAPAAEDAAALEEVTTPGTKTIATLAAFLGVPETRT
                     AKAGFFVSGDGRLITAIVRGDLEFNETKLSNAVGASTGLRPATVEEIRAAGMEPGYGS
                     PIGAHDTVVVVDELVARSPNLVAGANREGVHYRNVNVGRDFTPDVSADIANAREGDPC
                     PTCGQPVILRNGIEVGNIFKLGTKFSDAAGATYLGEDGREHPIVMGSYGIGVGRNVAC
                     VVEDHHDEKGIVWPEEVAPYAAHLVAIGANKEPRVTEIAERLHAAGEAAGYWRNILYD
                     DRDESPGVKFTDAELLGMPWVLTVSPRSLAAGGVEVTNRATGERSTRSIEDVEALIRG
                     A"
     gene            complement(3229..3798)
                     /locus_tag="VHM48_08990"
     CDS             complement(3229..3798)
                     /locus_tag="VHM48_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755586.1"
                     /translation="MPIVVRRGRVVAEPRSRLLRFERPAAIPTVATAVGSEPRAPDPR
                     HELAHLALDLVVRTAEPVDLPRRWATPMFGREVDRTRAQLAPIRSRAGLIASFSREAA
                     IAVTRSADGRDQPAGPVRVAYAIRWLELGDGRRRPAWESFVAGPVAIVAGAISGVVGP
                     VSARPGVCALAGPQNPVRAGDQRKGEITT"
     gene            4217..5071
                     /locus_tag="VHM48_08995"
     CDS             4217..5071
                     /locus_tag="VHM48_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2755587.1"
                     /translation="MRRAVSILALLALVGGVAATGVAPAATADAASNRPQEYLALGDS
                     LAFGYSPLVSPASAGNFIAYPDKVSAALRERLTNAACPGETSSHLIDLAGTDNNCGLW
                     RANFPLHVAYSTTQLAFADGFLQSHPKTLVVSIDIGANDAQALVNACGGQTEVGCIQA
                     GLPVMLATLSANLDTIYGHIRNLDGYHHKLVAVTTYSTNYGDPLPTSVISMVNQVVAE
                     RTLAWGGIVADGFGAFAAASAATGGDACAAGLLIAVSSAPLTCDSHPSPAGRDLLARA
                     IVNALRTD"
     gene            complement(5345..>5627)
                     /locus_tag="VHM48_09000"
     CDS             complement(5345..>5627)
                     /locus_tag="VHM48_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004105653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2755588.1"
                     /translation="GHRLLRDGEEVAIKPKAFELLAFLLRHPGQAFTRDQLLQHVWGY
                     DYAGETRTVDVHVHWLRSTLEDDAAEPRFIHTVRGVGYVFRRPGAALRA"
BASE COUNT          802 a         1980 c         2039 g          806 t
ORIGIN      
        1 ggctaacgcc atcggggctg gcgtatggga agaaacgtcc ggcgtcccgg cgttgtctcc
       61 agtcgtgcgg ctcagccggg ccggcggcgc gcccggggtc cgctggccgg gacgggcagc
      121 acgagcggag cccacggccc cgcgagccgt tccagctccg ccggcttgaa cgcgtgacgg
      181 aggaccaggc cgtggatcgc ccggccgagc gagcgccggg ccagggacgg tgtcgtctcc
      241 gggagcaggg cgagggcgtc gcgtcgcgcc agcgccgagg agactcgcag gccgtcgtcg
      301 tcggtcgggg tcaggccgcg cggccacggg agctcggcgg gaacgctgcc gcgcggcgcc
      361 aggccaccga tccgcgctcg ttcggccggg gcgagggccg cgaggcgtcg cagcgcgtcg
      421 gcgaccgccg ccgtgttcgg gccgtaccgc ggctgatcga cggccgcctg ccagccacgc
      481 gtcagccgct cccggacgcg ctcgcggaaa tccccggaca ggtgctcgtc gaggaacggc
      541 tcgaggacga tgtcctgggc gaagccgacg accgcgtcgc gtgtcgccgg atcccagcgc
      601 ccgcgcggcg gcaacgcgtc ctggaccatc ggctcgagcg tgcggagggc atcctggcga
      661 tcggcgggga ggaagcgcga gatcgtcgcg atgaaggcga tcggggccgt ctcagccagc
      721 gacaggcggt ccagctcgtc ttcggtcagg cgccggatgc ggtcgatgtg gcgggcgatc
      781 gcctgggccg ggttgaccgg acggcgaacg acggctgggc gccggacggg ccggtcgggg
      841 gccgacgatc gcacgctcgg cggcggaggc cgacgaccac cgaaggaggt cgccgccacc
      901 cggcgcggat ggcgttcgcg gtcgtagcgg gcccgcagct ccggatcgga cagccagtcg
      961 tgggcaacgt tgatccgttt cgccagctcg tcggccgtcg gtcccgcgat atcgggatga
     1021 tgctgcttca ggagcgcccg ccaggcgatc tcgatgacct cgtacgaggc gtcgatgggc
     1081 agctcgagcc gggcgtagag gtcgtcgggc gggagcatcg gccggggagg catcgtccca
     1141 tcctatcggg gcatccgacg cgacggggcc ggtcagcgac cctggtcagg cgccccggat
     1201 cagcgcctcg acatcctcga tcgagcgggt cgatcgttcg cccgttgccc ggttcgtgac
     1261 ctcgacgccg cccgccgcga gcgagcgggg cgacacggtg aggacccagg gcatcccgag
     1321 cagctcggcg tcggtgaact tgacgccagg cgactcgtcg cggtcgtcgt agaggatgtt
     1381 ccgccagtag ccggccgcct cgccggcggc gtggagccgc tccgcgatct cggtcacacg
     1441 aggttccttg ttcgccccga tcgcgacgag gtgggcggcg tacggcgcga cctcctcggg
     1501 ccagacgatg cccttctcgt cgtgatgatc ctcgacgacg caggccacgt tccgcccgac
     1561 gccgatcccg taggagccca tgacgatcgg gtgctcgcgg ccgtcctcgc cgaggtaggt
     1621 cgccccggcc gcatcgctga acttcgtgcc gagcttgaag atgttgccga cctcgatccc
     1681 gttgcgcagg atcacgggct ggccgcaggt cgggcaggga tcgccctcac gggcgttcgc
     1741 gatgtcggcg ctgacgtcgg gcgtgaagtc gcggcccacg ttgacgttgc ggtagtggac
     1801 gccctcgcgg ttggcgcccg cgacgagatt gggcgaacgg gcgacgagct cgtcgacgac
     1861 gacgaccgtg tcgtgggcgc cgatcggcga gccgtagccg ggctccatcc cggcggcccg
     1921 gatctcctcg accgtcgcgg gtcgaagtcc agtgctcgca ccgacggcgt tggacagctt
     1981 cgtctcgttg aactcgaggt cgccgcggac gatcgcggtg atgagccggc cgtcgccgct
     2041 gacgaagaag ccggccttgg ccgttcgcgt ctccgggacg ccgaggaagg cggcgagggt
     2101 cgcgatggtc ttcgtgccgg gggtcgtgac ctcctcgagc gcggccgcgt cctcggcggc
     2161 gggcgcgggg tgctcgatcc gggcgacctg gcggttggcc gcgtagccgc acgcctcgca
     2221 caggacgagg acgtcttcgc cggccgggtt gaggaccatg aactcgtggg ccccgctgcc
     2281 gcccatgatc ccgacgtcgc tgctgaccgc cacggtcttc aaccccagac gctcgaacgt
     2341 ccggacgtag gcgtcgtact gggcctggta gctcacgtcc aggccggcct cgtcccgatc
     2401 gcagctgtag gcgtccttca tcacgaactc gcggacgcgg atgaggccgg cccgggcccg
     2461 cggctcgtcg cgccacttcg tctggaagtg gtagaccatc atcggcagct ggcgatacga
     2521 cttcacgatg tcggcgagca ggatcccgac gacctcctcg tgggtcatcg cgaggaccat
     2581 gtcgcgcccg ttgcggtcct tgaagcgagt cagctcctgg ccgatggcgt cgtagcgacc
     2641 gctcgcccgc cagatgtcgg ccggatggac gaccggcatc tccatctcct ggccgccgat
     2701 ccggtcctgc tcctcgcgga tgacctgctc gacgcggtca ttgacccgct tcccgagggg
     2761 caggagcgag tagatcccgg aacccagcgg gcggatgtag ccggcccgga ggagaagccg
     2821 gtgcgacggc atctcgacat cggcgggatc gtcgcggagg gtcgtgaaga agaggtggga
     2881 gaggcgcatc ggcgaagaat agcccgagca ggcgccgatc cgcggagccc acgccgaggc
     2941 cgacgcccag ccgcccgcca atcaggtagc cttcggccgg gtccaccggt ctgaccctgc
     3001 tgctgcttgc cgtcatcggg ctggcggtcc tcttcgacta catcaacggc ttccacgaca
     3061 cggcgaatgc gatcgcgacg tccgtctcga cgcgggccct ccgcccggag cacgccatcc
     3121 tcatgtcggc gacggcgaac ttcgtcggtg cgttggccgg gacggcggca acatcgtgat
     3181 cgcgtggatc ctgacgatcc ccgcgtcggc cctggccgcg gcggtcgcct acgtcgtgat
     3241 ctcgcccttc ctctgatcgc cagccctgac cgggttctgc gggcccgcga gggcgcacac
     3301 gcccgggcga gcggacacgg gaccgacgac accagagatc gctccagcga cgatcgcgac
     3361 cggacccgcg acgaacgact cccaggccgg ccggcgtcgc ccgtcgccga gctccagcca
     3421 gcggatggcg taggccaccc ggacggggcc tgccggttgg tccctcccgt cggcactgcg
     3481 ggtgaccgcg atggccgcct cccgcgagaa cgaggcgatc agcccggccc gcgatcggat
     3541 cggcgcgagc tgggcccgcg tccggtcgac ctcgcggccg aacatcggcg tcgcccagcg
     3601 gcggggcagg tcgaccggct cggcggtccg gacgacgagg tcgagagcca ggtgggcgag
     3661 ctcatgccgc ggatccggcg cccgaggctc gctgccgacc gccgtcgcga cggtcgggat
     3721 ggccgccggg cgttcgaacc ggaggagtcg ggaacgcggc tcggcgacga cgcggccgcg
     3781 gcggacgacg atcggcatgg gcgaagggtc gcggcggcgg atcaacggcc cgtgatcaag
     3841 caatcaacgc tcggacggga tccggccggg catcgtgggc ctcgggcgcg cggcggcgcc
     3901 cctggggcgg cgggatcagg gaatccgcct cacgtcctct ggcagactcg caccaggaga
     3961 ccagggcgca tccgcccgac gaggaccagc gacgattcgg gacaggatcg ccgccttcgt
     4021 cgcaagactc ggcctgccgc cgccctgcga cgggacgacc gggccattgg cctctggcgc
     4081 aaggcgctcg accggccgat cgtccgtgct ggaaagcggg aggttctgac gcgcggccca
     4141 tgccctggtc gcccggaccg cgccgagcca ccggcgaaac cgacccgccg atggatgcgt
     4201 ccccggaggg tttgcgatgc gtcgcgccgt ctcgatcctt gcgctgctgg cccttgttgg
     4261 cggggtggcc gccacgggag tcgctcccgc tgccacggcc gatgctgcct ccaaccggcc
     4321 gcaggaatac ctcgcccttg gcgactccct cgccttcggc tacagcccac tggtcagtcc
     4381 ggcaagcgcc ggcaacttca tcgcataccc cgacaaggtg tctgccgcgc tccgtgagcg
     4441 cctcacgaat gccgcctgcc cgggtgagac gagcagccac ctcatcgatc tcgccggtac
     4501 cgacaacaac tgcggtctct ggcgtgccaa cttcccgctc catgtggcct acagcacgac
     4561 gcagctcgcg tttgccgatg ggttcctcca gtcccatccc aagacgctgg tggtcagcat
     4621 cgacatcggg gccaacgacg ctcaggccct ggtgaatgcg tgtggcgggc agaccgaggt
     4681 cggctgcatc caggcgggct tgccggtcat gctggcaacc ctctcggcca accttgacac
     4741 catctacggg cacatccgca acctggatgg ctatcaccac aagctcgtcg cggtgacgac
     4801 ctactcgacc aactacggtg accccctccc gacgtccgtc atctcgatgg tcaaccaggt
     4861 ggtggctgaa cgcacgctgg cctggggcgg gatcgttgcc gacgggttcg gcgcatttgc
     4921 cgccgcgtcc gctgccaccg gtggggacgc ctgtgcggcg ggattgctca tcgcggtgtc
     4981 atcggccccc ctcacctgtg acagccaccc gtcgccggca gggcgggacc tgctggcgcg
     5041 ggccatcgtc aacgcgctgc gcaccgactg agccgttgga gggcgccggg cgctgcccac
     5101 ctgctctcct ctgaactcgc acgagaggat gatccccggc ggcacgacgc cggggatcat
     5161 cgattgcagc agaacccaag gcaggccgac acgcagcgcc gccaggcgag cagctcagac
     5221 agcccgccgc gccggaccga gccgtcggct gcggcggact gaggaccgtg gtctggcgac
     5281 cggggccgct caatgtccat tggccagcag atcggtttga ctcgacgttc ttgcgtccct
     5341 ggactcaggc gcgcagcgcg gcccccggcc ggcggaacac gtaaccaacg ccgcggaccg
     5401 tgtggatgaa ccgcggctcc gctgcgtcgt cctcgagcgt cgagcgcagc cagtggacgt
     5461 ggacgtcgac ggtccgcgtc tcaccggcgt agtcgtagcc ccagacgtgc tggaggagct
     5521 ggtcgcgggt gaaggcctgg cccgggtggc ggaggaggaa cgcgaggagc tcgaacgcct
     5581 tcggcttgat cgcgacctcc tcgccgtcgc gaaggaggcg atgcccg
//
LOCUS       DATAIW010000287         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_77923, whole genome shotgun sequence.
ACCESSION   DATAIW010000287 DATAIW010000000
VERSION     DATAIW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77923"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VHM48_09005"
     CDS             <1..514
                     /locus_tag="VHM48_09005"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755589.1"
                     /translation="VTPLRGLRLAAGERPPGLLLVTPAVAAHTAAVFAAWAGGAMGQP
                     GVAARSSEHFAAEFGSGMSARQFLERAGVLAASNDLLPAAAAVVDGLVALRRALARLL
                     ARPIGLSGSGPTLWALYPSTDDADLAAASVRAAVATGFITAPGEGPPFVAVTTFLIHT
                     AEPDDQRSDP"
     gene            511..921
                     /locus_tag="VHM48_09010"
     CDS             511..921
                     /locus_tag="VHM48_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2755590.1"
                     /translation="MTRQAIQTNGAPSAIGPYSQGIRSGEFVFCSGQLGLDPATGELA
                     EGIEAQAERSLRNLQSVLDAAGLTFDDVVKTTIFLADINDFATVNAVYAKFMPDPPPA
                     RSTIGVGALPKGGLVEIEAIARRPLPDAVSDRRA"
     gene            complement(1317..1745)
                     /locus_tag="VHM48_09015"
     CDS             complement(1317..1745)
                     /locus_tag="VHM48_09015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2755591.1"
                     /translation="MSNCITCDVHARRIHPAGGVIFESDLWRAEHGVDRLVRGYVVLK
                     PKRHVHELADLHPAESATLGPALQPLLRAMRSALDAERIYVCSFAETVHHLHFHLIPR
                     YADMPSLGPNLLADVFAQRWGCTVEEAEDAASRIRMALTA"
     gene            1947..>3137
                     /gene="glmU"
                     /locus_tag="VHM48_09020"
     CDS             1947..>3137
                     /gene="glmU"
                     /locus_tag="VHM48_09020"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX2755592.1"
                     /translation="MIAVVMAAGLGTRMKSRTPKLVHQLAGRPMVAYVVDAGREATGR
                     RPLVVTSPATAAVRDALGDAVDYALQAEPRGTGDAVAAALAAIPDGIDEVLVLTGDAP
                     LLEAPLLAELLEERRLDEAAIALVVVDAIDPAHLGRVIRDDAGTVERIVERKDATDEE
                     LAINEINSGLYVFDAAWLRSRIGSLSPSPKTGELYLTELVELARADGRLVVGLTVEDD
                     GRLLGINDRAELAQAEWDLRTRINSAHMRAGVTMRDPSTVYLDASVILAEDVTIEPNV
                     ILRGSTSVGAGTTIGAGSEVVDSTIGAGCLVWASIVERSVIEDGARIGPMSHLRPGSH
                     VGPDAEVGNFAELKNTMLGRGVKQHHMSYLGDAELGEATNVGAGTITANFDGRKKHRT
                     TIGKR"
BASE COUNT          467 a         1161 c         1065 g          444 t
ORIGIN      
        1 cgtcacgccc cttcgcggcc tgcgcctggc cgcgggcgag cggccgcccg ggttgctcct
       61 cgtgacgccg gccgtcgccg cccacacggc ggcggtgttc gccgcctggg cgggcggggc
      121 gatgggccaa cccggtgtcg ccgcccggag ctccgagcat ttcgcggcag agttcgggtc
      181 ggggatgagc gcgagacagt tcctcgagcg ggcaggcgtc ctcgccgcct ccaacgacct
      241 gctgccggct gccgccgccg tcgtcgacgg cctcgtcgcg ctgcgccgcg ccctggcccg
      301 actcctcgcc cggccgatcg ggctgtcggg ctcaggcccg accctctggg cgctctatcc
      361 ttcaaccgac gacgccgacc tcgccgccgc ctccgtccgg gcggccgtcg cgaccggctt
      421 catcaccgcc ccgggggagg gtccgccctt cgtggcggtc accacgttcc tgatccacac
      481 cgccgagccc gacgaccaga ggagcgaccc atgacccgcc aggcgatcca gaccaacggc
      541 gccccatcgg cgatcggccc gtacagccag gggatccgga gcggcgagtt cgtgttctgc
      601 tccggccagc tcgggcttga ccccgcgacc ggtgagctcg ccgaagggat cgaggcccag
      661 gccgagcgct ccctgcgcaa cctccagtcg gtcctcgacg cggccggcct gaccttcgac
      721 gacgtcgtca agacgacgat cttcctcgcc gacatcaacg acttcgcgac cgtcaacgcc
      781 gtgtacgcga agttcatgcc ggatccgccg cccgcccgct cgacgatcgg cgtcggcgct
      841 ctcccgaagg gcggcctcgt cgagatcgag gcgatcgccc gccgcccgct cccggacgcg
      901 gtctccgatc gccgtgcctg acgacccgat caggccgcgc gactggatct tcgggcttgt
      961 attcgtcgcg attgtcctgg cggcgttcat cgtggtcgtc tggttgaacg tcgctccgcg
     1021 ctcggtctga ccgtttcgga ggccggatcg acgggcacga cagggcgacg tgcatccaat
     1081 gaaccaatgc gacgtcccgg acacccgcgc cgtcgtccgc tgttcggatg ggcgcatcga
     1141 atgcacgaaa ccgaccgaat tcgtccgccc gaggagcgaa tcgaccctca agggcctcga
     1201 ttcggtcgga taggttcatc tgacgcacgc gaatcccgga atggcgcatt ggatgcgcca
     1261 acagagtggc gacggacggc gcggcttctg acccaagccg ctggcgccgt tgcgcgtcaa
     1321 gccgtcaggg ccatccgaat ccggctggcg gcgtcctcgg cctcctcgac cgtgcatccc
     1381 cagcgctgag cgaacacatc ggccagcagg ttgggaccga gtgacggcat gtccgcgtat
     1441 cgcgggatca agtgaaaatg gagatggtgg accgtctcgg cgaacgagca gacgtagatt
     1501 cgctcggcat cgagcgcgga tcgcatcgcg cgaagcagcg gctggagcgc gggcccgagc
     1561 gtggcggact ccgctggatg gagatcggcg agctcgtgaa cgtgtcgctt cggcttcagg
     1621 accacgtagc cgcggacgag tcgatccaca ccgtgctccg cgcgccagag atcgctttcg
     1681 aagatcaccc caccggcagg atggatgcga cgcgcatgca catcgcaggt gatgcagttg
     1741 gacacggccc gagtctatcg ccctccagcg tcggctcaac gtcggttgac ggcggcggcg
     1801 ggccgcccat accatcgcgc ctacccttcg atgacatcaa cacctcgaca cgcgccgggc
     1861 cctgaccgcg tcgggatcgc ttccccgacc ctcgcccgct cgcgtcgccc ctcgtgaacc
     1921 ctccaagtcc cggcgcgtcc ccgcgcgtga tcgccgtcgt catggcggct ggcctcggca
     1981 cgcggatgaa gtcgcggacg ccgaagctcg tccaccagct ggccggtcgg ccgatggtcg
     2041 cctacgtggt cgacgccggc cgcgaagcca ccgggcggcg cccgctcgtc gtcacgagcc
     2101 ccgcgaccgc cgccgtccgc gacgccctcg gcgatgccgt cgactacgcc ctccaggccg
     2161 agccgcgggg cacgggcgac gccgtggcgg cggccctcgc agccatcccg gacggcatcg
     2221 acgaggtcct cgtcctcacc ggcgacgcgc ccctcctcga ggcgcccctg ctcgccgagc
     2281 tgctcgagga gcgccgcctg gacgaggctg ccatcgccct cgtcgtggtc gacgcgatcg
     2341 accccgccca tctcggccgg gtcatccgcg acgacgcagg cacggtcgag cggatcgtcg
     2401 agcgcaagga cgcgaccgac gaggagctcg cgatcaacga gatcaactcc gggctgtacg
     2461 tgttcgacgc ggcctggctg cgcagccgga tcggctcact gagcccgtcg ccgaagaccg
     2521 gcgagctgta cctgaccgag ctcgtcgagc tcgcccgcgc cgacggccgg ctggtcgtcg
     2581 ggctgacggt cgaggatgac ggacgcctcc tcgggatcaa cgaccgggcc gagctcgccc
     2641 aggccgagtg ggacctccgg acccggatca acagcgccca catgcgggcc ggcgtgacga
     2701 tgcgcgaccc ctcgacggtc tatctcgacg cgtcggtgat cctcgccgag gacgtcacga
     2761 tcgagccgaa cgtgatcctg cgcggctcga cgtcggtcgg ggcggggacg acgatcgggg
     2821 ccggctcgga ggtcgtcgac agcacgatcg gcgccggctg cctggtgtgg gcgtcgatcg
     2881 tcgagcggtc ggtcatcgag gacggcgccc ggatcgggcc gatgagccac ctccgaccgg
     2941 gctcgcatgt cgggccggac gccgaggtcg gcaacttcgc cgagctcaag aacaccatgc
     3001 tcgggcgcgg cgtcaagcag caccacatga gctacctcgg cgacgccgag ctgggcgagg
     3061 cgacgaacgt cggcgccggc acgatcacgg ccaacttcga cggccgcaag aagcaccgga
     3121 cgacgatcgg caagcgg
//
LOCUS       DATAIW010000288         4061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_77964, whole genome shotgun sequence.
ACCESSION   DATAIW010000288 DATAIW010000000
VERSION     DATAIW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77964"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHM48_09025"
     CDS             complement(<1..295)
                     /locus_tag="VHM48_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2755593.1"
                     /translation="MTSVAEPTASHAPISSAPGSDPDKATIAVRNLWKVFGPAESRLA
                     SDSTTIPSAESIDATVRKYGGTIAVGDVSFDVEPGEVFVVMGLSGSGKSTLVRC"
     gene            complement(368..727)
                     /locus_tag="VHM48_09030"
     CDS             complement(368..727)
                     /locus_tag="VHM48_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755594.1"
                     /translation="MLIIWRGWGLLAILFPLISVLVANVVVDVVAGAGAFGTSSVGPG
                     LGLVAAGTLCWVTGRRVNGEPVPYLGRSVADGQPFRPRLPFLFARHNFLFVPLEAWGV
                     LFFGLGLLAVVRGVLHV"
     gene            967..3492
                     /locus_tag="VHM48_09035"
     CDS             967..3492
                     /locus_tag="VHM48_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2755595.1"
                     /translation="MSSVPTHARVVIIGAGIVGCSMAYHLARLGWRDMVLLEKGMLPN
                     PGGSTGHASNFIFLTDHSKEMAAFTIESARQYHELGVYDETGGIEVARTPARMEELKR
                     RMASSTSWGVEGSRLLTPAEVKAMVPFIDETVILGGFYTPGVGIVDSLQAGTLMRQAA
                     QEMGALTTAASVEVTGIDVEDGRVRRVHTDAGTIDADVVVIACGVWSPRIARMAGASI
                     PLTPAVHQMMDIGPVPMFEHTTREVGYPIVRDMDTNMYERQHGTGFEIGSYAHRAILM
                     DADDIPSIAASALSPTMLPFTKEDFDPQLEDSLELFPEIVGDESVGVKLAINGLLSLT
                     PDGNPIIGETPEVRGLWSVAAVWIKEAPGIAKTVAEWMTNGEPEIDPHGSDIARFYDH
                     HKTAQHIAARTTEGYNKTYGIVHPMEQWASNRPIRLSPAYDRHVELGAEFFEAAGWER
                     PFWYATNESLLGEYGDRVMPREAEWESRWWSPIINAEHLAMRDRVGLVDLSAFAIFDI
                     TGPGALAALERLCVNKVDVAVGRTVYTPLLNAAGGILADLTIMRLAHDRFRVVTGGGM
                     GMRDRKIFQDGLPADGSAQLHDQTNAFTTFGLWGPQARDVLGAAADGSADFSHKGFPF
                     LSSKTVDIDGVRTLASRISYVGELGWEIYVPIEQGLRVWDALWRAGRPHGLAPVGIGT
                     YAVTSRLEKGYRAHGAELELDFDLVEAGMARPTLKDADFVGRAAYERQRATDPAAILC
                     TLTVDDPTSSTGVKRYMLGREPILTLDGRRIVDAKGRGSYVTSAGSGPSVDKHLLMSY
                     LPPEHATAGTKLLVEYFGEHYPVTVAVAGSTPLFDPENTRIRS"
     gene            3500..>4061
                     /locus_tag="VHM48_09040"
     CDS             3500..>4061
                     /locus_tag="VHM48_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755596.1"
                     /translation="MKVLVCVKRVPATGGRIVLTPDGSDIDVRYLGFTVSPHEECAVE
                     EAVRIVEAHGGASAVLTLGPAAAEEQLRDAMAIGVDRAILLETDGRDWDPIATAGAIV
                     EAIRAKEAADGPFDLILFGNESADSGGFQVGIRVAASLDRPCVTGVKSLQPGDGRIVA
                     RREAPSGGWESFEVPLPAVVSVKEGIN"
BASE COUNT          662 a         1424 c         1418 g          557 t
ORIGIN      
        1 ggcagcggac gagggtcgac ttgccgctgc cggacaggcc catgacgacg aagacctcgc
       61 cgggctcgac gtcgaacgag acgtcgccga cggcgatggt cccgccgtac ttgcggaccg
      121 tggcgtcgat cgattcggcg ctcgggatcg tggtcgagtc gctcgccagg cgactctcgg
      181 ccggcccgaa caccttccag aggttgcgga ccgcgatcgt ggccttgtcg gggtccgaac
      241 ccggcgcgga cgagatgggc gcgtgcgagg cggtcggctc ggcaacggag gtcacggcat
      301 gctcctgctc ggtgacgcga gacgtgcggg gacggtacac caaacgagtg cccgaacggc
      361 accccgttca gacgtgcagc acgcctcgaa cgacggcgag caggccgagg ccgaagaaca
      421 gaacgcccca ggcctcgagc gggacgaaga ggaagttgtg gcgcgcgaac aggaacggaa
      481 ggcggggacg gaacggctgg ccgtctgcga cggagcggcc gaggtacggg accggctcgc
      541 cattcacccg ccgccccgtg acccagcaca gggtcccggc ggcgaccaac ccgagcccgg
      601 ggccgaccga ggacgttccg aacgctccgg cacccgccac cacgtcgacg acgacgttcg
      661 cgacgagcac cgagatcagc ggaaacagaa tggcgaggag cccccagccg cgccagatga
      721 tgagcatggc aagcgcgatc ctactgcagg gatcgattgc agatgcgcgc atcacgaccg
      781 tcacgacggc gcgtcctgcg atgagcgaca gccagcctcg ggctcgatgg acggcctgcg
      841 tcgtccgccg accggcgttc gggcggagtc ccgcagggcg catcgccatc gtcgtccatc
      901 gacggcccaa ccggcggcga gcgatacttc cgccctgccc gtcacgaacg agaggattcc
      961 cacggcatga gctcggtccc gacgcacgcc cgcgtcgtca tcatcggtgc cggcatcgtc
     1021 ggctgcagca tggcctacca cctcgcccgc ctcggttggc gcgacatggt cctcctcgag
     1081 aaggggatgc tgccgaaccc cggcggctcg accggccacg cctccaactt catcttcctg
     1141 accgatcaca gcaaggagat ggccgccttc accatcgaaa gcgcccgcca ataccacgag
     1201 ctcggcgtgt acgacgagac cggcggtatc gaggtcgcgc gaacgcccgc ccggatggag
     1261 gagctcaagc gccggatggc ctcgtcgaca tcgtggggcg tcgagggcag ccggctgctg
     1321 accccggccg aggtcaaggc gatggtcccg ttcatcgacg agaccgtgat ccttggcggc
     1381 ttctacacgc ccggcgttgg gatcgtcgac tcgctccagg ccgggacgct gatgcgccag
     1441 gccgcccagg agatgggcgc gctgacgacc gcggcgagcg tcgaggtcac cgggatcgac
     1501 gtcgaagacg gccgcgtccg gcgggtccac accgatgcgg gaacgatcga cgccgacgtc
     1561 gtggtcatcg cctgcggcgt ctggagcccg cggatcgcgc ggatggccgg cgcctcgatc
     1621 ccgttgacgc cggcggtcca ccagatgatg gacatcgggc ccgtcccgat gttcgagcac
     1681 accaccaggg aagtcggcta cccgatcgtc cgcgacatgg acacgaacat gtacgagcgg
     1741 cagcacggca ccggcttcga gatcgggtcg tacgctcacc gggcgatcct catggacgcc
     1801 gacgacatcc cgtcgatcgc cgcgtccgcg ctgtctccga cgatgctgcc gttcaccaag
     1861 gaggacttcg acccccagct cgaggactcg ctcgagctgt tcccggagat cgtcggcgac
     1921 gaatcggtcg gggtgaagct ggcgatcaac gggctgctgt cgctcacgcc ggacggcaac
     1981 ccgatcatcg gcgagacgcc cgaggtcagg ggcctctggt cggtggccgc ggtctggatc
     2041 aaggaggcgc cgggcatcgc caagacagtc gccgagtgga tgacgaacgg cgagcccgag
     2101 atcgatcccc acggctcgga catcgcccgc ttctacgacc atcacaagac cgcccagcac
     2161 atcgccgccc ggacgaccga gggctacaac aagacctacg gcatcgtcca cccgatggag
     2221 cagtgggcgt cgaaccggcc gatccgcctg tcgcccgcct acgaccgcca cgtcgagctc
     2281 ggcgcggagt tcttcgaggc agccggctgg gagcggccct tctggtacgc cacgaacgag
     2341 tccctgctcg gcgagtacgg cgaccgggtc atgccgcgcg aggccgaatg ggaatcgcgc
     2401 tggtggtcgc cgatcatcaa cgccgagcac cttgcgatgc gcgaccgggt cgggctggtc
     2461 gacctctcgg ccttcgccat cttcgacatc accgggccgg gcgcgctggc cgcgctcgag
     2521 cggctctgcg tgaacaaggt cgacgtggcc gtcgggcgga ccgtctacac gccgctcctg
     2581 aacgcggccg gcgggatcct cgccgacctg acgatcatgc gcctggccca cgaccggttc
     2641 cgggtcgtga ccggcggcgg gatggggatg cgcgaccgga agatcttcca ggacgggctg
     2701 ccggccgacg gctcggccca gctccacgac cagacgaacg ccttcacgac gttcggcctg
     2761 tggggaccgc aggcccgcga cgtcctcggc gcggccgccg acggcagcgc cgatttcagc
     2821 cacaagggct tcccgttcct gtcgtcgaag accgtcgaca tcgacggagt ccggacgctc
     2881 gcgtcgcgga tcagctacgt cggtgagctc ggctgggaga tctacgtccc gatcgagcag
     2941 ggccttcgcg tctgggacgc cctgtggcgg gccggccggc cgcacggcct ggccccggtc
     3001 gggatcggca cgtacgcggt gacctcccgg ctcgagaagg gctatcgcgc ccacggcgcc
     3061 gagctcgagc tcgacttcga cctcgtcgag gcgggcatgg cccggccgac tctcaaggac
     3121 gccgacttcg tcggccgggc ggcctacgag cgccagcgcg ccaccgaccc ggcggcgatc
     3181 ctctgcacgc tcaccgtcga cgacccgacc tcgtcgaccg gcgtgaagcg ctacatgctc
     3241 ggtcgcgagc cgatcctgac cctcgacggc cgccggatcg tcgacgcgaa ggggcgcggc
     3301 tcgtacgtga cgagcgccgg ctccggaccg tcggtcgaca agcacctcct gatgtcgtac
     3361 ctgccgcccg agcacgcgac ggcggggacg aagctcctcg tcgagtactt tggcgagcac
     3421 tatcccgtga ccgtggccgt tgccggcagt acgccgctat tcgaccccga gaacacccgg
     3481 atccggtcct gatcctgcga tgaaggtcct cgtctgcgtc aagcgggtgc cggccaccgg
     3541 cggccggatc gttctgactc cggacgggtc cgacatcgac gttcgttacc tcgggttcac
     3601 cgtcagcccg cacgaggaat gcgcggtcga ggaggcggtc cggatcgtgg aggcgcacgg
     3661 tggcgcgagc gccgtgctga cgctcggtcc ggccgcggcc gaggagcagc tccgcgacgc
     3721 gatggcgatc ggcgtcgatc gggcgatcct gctcgagacc gacgggcgtg actgggaccc
     3781 gatcgcgacg gccggcgcga tcgtcgaggc gatccgggcc aaggaggccg ccgacggacc
     3841 gttcgacctg atcctcttcg ggaacgaatc ggccgacagc ggcgggttcc aggtcgggat
     3901 ccgggttgcc gcctcgctcg atcggccgtg cgtgacaggg gtgaagtcgc tccagcccgg
     3961 cgacggccgg atcgtcgcgc ggcgggaggc gccaagcggc ggctgggagt ccttcgaggt
     4021 ccccctgccc gcggtcgtct cggtcaaaga agggatcaac c
//
LOCUS       DATAIW010000289         6761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_78008, whole genome shotgun sequence.
ACCESSION   DATAIW010000289 DATAIW010000000
VERSION     DATAIW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78008"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1354
                     /locus_tag="VHM48_09045"
     CDS             269..1354
                     /locus_tag="VHM48_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755597.1"
                     /translation="MRVAAIGVLIAVGPAAGPVIARSHTAHTAPDAVSVFAGAPAPGH
                     PFGIAVGDGRVYVSTSAGDFFADPANGGHRSDDERIFTYDEAGNLVDTTVIDTMDDAT
                     MGLFGLALDGNPGPRHQLYAADMNGRILTVGLGAHPAPPKVFSQVPEDSGLAGDWMLS
                     MWNDLVFDKAGNLYVPDDKPRIWRVGTDGTAHIWFTDPRLTGLFGFAGGPLGGRIDPT
                     GQWLYISITLPAEFPFDATIYRIRLVDHPTASDMQLVHRFVASPDPTVAPPQATGLAF
                     AKSGNLYVSLLGPNQVAVLDPAGNEIRRISDPRFFSPWGLAFLGNSLLVTNGDLEPAD
                     NPAAWKIFKVAVGETGLSLNRPKVPGN"
     gene            complement(1433..1723)
                     /locus_tag="VHM48_09050"
     CDS             complement(1433..1723)
                     /locus_tag="VHM48_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755598.1"
                     /translation="MPVLQIEHPVRDYAAWKAAFDSDPAGRERGGVRRYEIYRPVDDP
                     GFVAVDLAFDNRDAAEAFKLGLESLWRTPRASAALAGTPRARIVDVVEDREY"
     gene            1877..2497
                     /locus_tag="VHM48_09055"
     CDS             1877..2497
                     /locus_tag="VHM48_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2755599.1"
                     /translation="MIEAARALFVDLGYVATTINAIAERADVAPETVYASFGNKRSLL
                     AALVDVSIAGDATAAPILEQDWVRTMGDEPDARRRLRILAGQGRSILERRSVVDEIVQ
                     GAAAADAEIAALRDRGRAQRFEGQRRLLAVVVGTTGLRKGLDVETATDVLYALGSPET
                     WRLLVVDRGWTADEFEDWYAETLERLFLEPDSTATATAADAADPTT"
     gene            complement(2550..2948)
                     /locus_tag="VHM48_09060"
     CDS             complement(2550..2948)
                     /locus_tag="VHM48_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2755600.1"
                     /translation="MIFVDSNIPMYLVGADHPNKTAARRLLEQAIVDNEPLSTDAEVL
                     QELLHRYTAISRRDAIGPAWDAILGVVDIVHPIEQEDVARARRLVGSAASLSARDAVH
                     LAVMQRRDISRILTFDAGFDGIVGIERIRP"
     gene            complement(2945..3202)
                     /locus_tag="VHM48_09065"
     CDS             complement(2945..3202)
                     /locus_tag="VHM48_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755601.1"
                     /translation="MTKRLQVLFEDDELRELQRLARQHRMTTAEWVRRSLRAARDAEA
                     GADVGQKLAAVRAAAAFSFPTAEIGVMLDEIERGYGQADSP"
     gene            complement(3341..4783)
                     /locus_tag="VHM48_09070"
     CDS             complement(3341..4783)
                     /locus_tag="VHM48_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX2755602.1"
                     /translation="MAAPRPAPDHRVLWTFAISSIALFMAALDNLVVTTALPVIRASF
                     NANLAELEWIVNAYTLTFAVLLLTGAALGDRFGRKRLFIIGLAIFTGGSVIAALSSNV
                     TVLIVARAVQGLGGAILTPLSLTILSAAVPRERRAVALGAWGGIAGLAIAIGPLVGGA
                     ISEGLAWQAIFWINVPIGLIAIPMAYARLSETRGPSTRLDLPGLGFVSGGLLAVVWGL
                     VRANEQGWTSLEILGSFAVGIVLLASFVAWEARSAEPMLPLRLFRSRSFTAANVVSML
                     MTFGMFGSIFLLAQFFQVVQHYSPLQAGLRTLPWTIMPVFVAPIAGLLSARTGTRPLL
                     VLGMSLQAIALGWMSFVVTPTVDYGAIVPSFILAGIGMGLFFAPIANVVLSAVRPEEE
                     GKASGANNAIRELGGVFGVAVLASIFSANGGYASGQTYVDGLVPAMQVGAVVVAIGAL
                     AALALGGRTRVVVAAAEAYDDFVGVGAAAA"
     gene            complement(4861..5400)
                     /locus_tag="VHM48_09075"
     CDS             complement(4861..5400)
                     /locus_tag="VHM48_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2755603.1"
                     /translation="MTADERRDAIVAAGVEEFATGGLVGASTDAIARRAGVSQPYVFQ
                     LFGTKKELFLAVVRRCFERTRLTFERAAGDFVPGENSQCNTVLDAIGMSYHGLLADRN
                     QLLVQLQAYAACADPDVQVVVREEFGRLHRRVRELSGADPMDIHRFFAEGMLLNIAAA
                     VEFSGDPHSWILAQMEGDA"
     gene            5423..5653
                     /locus_tag="VHM48_09080"
     CDS             5423..5653
                     /locus_tag="VHM48_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755604.1"
                     /translation="MERVGEVMTHNLVTTKSVVKSNVERGRQRTARGSLTDPSMVVAF
                     AVVGLIGALLAIRGVIPLRSAATRAGAAAGAR"
     gene            5889..6263
                     /locus_tag="VHM48_09085"
     CDS             5889..6263
                     /locus_tag="VHM48_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755605.1"
                     /translation="MQTDRLFRITAIAWLATAVARVALAAVQAGTPGPGVSPVAFAIG
                     AGAGLLVAGLLWFRPGRGSAIAACAFGLFAVVGIAYLPFIGFQPWFIVLSLADLIAFV
                     LSVVCLFATRRPGRSSDADRWA"
     gene            6260..>6761
                     /locus_tag="VHM48_09090"
     CDS             6260..>6761
                     /locus_tag="VHM48_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755606.1"
                     /translation="MSLLGGIADRVTARLPTMDRRTAIVLSLAVVLVPLVFVLVVPHP
                     LEQPFGVDFQLYRDVTTRWLAGGSFFEPYQLAGPYDIRAGDVLYPPVALWLFVPFAAA
                     GGVLASLAAIGWWLVPIGITAAAVVRLRPRPAVWPLIALCLANPTTLLKIWTGNPVMW
                     SMAAMAV"
BASE COUNT         1022 a         2344 c         2381 g         1014 t
ORIGIN      
        1 ggggagggat caccgggcat tcgaacgtcc tgggaggtgg ggagtgcctg ctcattgtcg
       61 cccgcccgcg ctccgcgcgt gggccggcct gtcgattccg ccgtccccgg atcgtcgtcc
      121 tcgtgacgga agcgagacgg acatggccgg cctcgcccga agcgaggagg ggcgtccgat
      181 gcggacccac agcgatattc ggcaatccga aacagtctgg accggtgaca cagcaagggg
      241 cccgggcagt cgaccgcggt gggtcgcggt gcgggtcgcc gcgatcggcg tcctgatcgc
      301 ggtcggcccg gccgccggcc cggtgatcgc ccgcagtcac accgcgcata cggcccccga
      361 cgcggtctcg gtcttcgccg gtgctcccgc cccgggtcat ccgttcggga tcgccgtcgg
      421 cgacgggcgg gtctacgtct cgaccagtgc cggggacttc ttcgccgacc cggccaatgg
      481 cggtcaccgc agcgacgacg agcggatctt cacctacgac gaggccggca acctcgtcga
      541 cacgaccgtc atcgacacga tggatgacgc aacgatgggc ctcttcgggc tcgccctcga
      601 cggcaacccg ggtccgcgcc atcagctcta tgccgccgac atgaacgggc ggatcctgac
      661 ggtcgggctg ggcgcccacc cggctccgcc gaaggtgttc tcgcaggtcc ccgaggacag
      721 cggtctcgcg ggcgactgga tgctctcgat gtggaacgac ctcgtgttcg acaaggccgg
      781 caacctgtac gtgccagacg acaagccgcg gatctggcgg gtcgggaccg acggtaccgc
      841 ccacatctgg ttcaccgatc cgcgcctgac cgggctcttc ggcttcgccg gcggcccgct
      901 cggcggccgg atcgacccga ccggccagtg gctctacatc tcgatcacgc tgccggcgga
      961 gttcccgttc gacgcgacca tctaccggat ccgcctcgtc gaccacccga cggcgtccga
     1021 catgcagctc gtccaccgct tcgtggcatc gcccgatccg acggtcgcgc cgccgcaggc
     1081 gaccggcctc gcctttgcca agtcgggcaa cctctacgtg agcctcctcg gcccgaacca
     1141 ggtcgcggtc ctcgatccgg ccggcaacga gatccggcga atctccgatc cgcggttctt
     1201 ctcgccgtgg ggtctcgcgt tcctcgggaa ctcgctgctc gtgacgaacg gcgacctcga
     1261 gcccgccgac aaccccgctg cctggaagat cttcaaggtc gccgtcggcg agacgggcct
     1321 gtcgctgaac cggccgaagg tgcccgggaa ctgagaccgg gcctggtcat cgccttcacg
     1381 agttcgagcc cgggttcggc catgccgagc ccgggcccta ccgtggccgg atctagtact
     1441 cccggtcctc gacgacgtcg acgattcgcg cgcggggcgt gcccgccagc gccgccgagg
     1501 cgcgaggcgt gcgccagagc gattcgaggc ccagcttgaa cgcctcggcg gcgtctcgat
     1561 tgtcgaatgc gaggtcgacg gccacgaaac ccgggtcatc gacgggtcga tagatctcgt
     1621 agcggcggac tccgccccgc tctcgccccg ccggatccga atcaaaggcg gccttccacg
     1681 cggcatagtc gcggacggga tgttcgatct gcagcacggg cacgggtcgc tccacactct
     1741 cgccggcagg gccggtaact acagtgacac tgtattgacc aagtgctact gtacacatat
     1801 gccgcgggac gtcaagacgc cggccagccc ggcctggccc cgccgccggc ttcgcgccca
     1861 ggccacgcgg tcgcgcgtga tcgaagcagc gcgggccctc ttcgtcgatc tcggctatgt
     1921 cgcgacgacg atcaacgcga tcgcggagcg ggcggacgtc gcgcccgaaa cggtgtacgc
     1981 gtcgttcggc aacaagcgat cgctgctcgc cgccctggtc gatgtctcga tcgcgggcga
     2041 cgcgacggcc gcaccgatcc tcgaacagga ctgggtccgc acgatgggcg acgagcccga
     2101 tgcgcggcgc cggctccgga tcctggccgg ccagggccgg tcgatcctgg aacgacgatc
     2161 ggtcgtcgac gagatcgttc agggcgcggc ggccgccgac gccgagatcg ccgcccttcg
     2221 cgatcgtggc agggcccaac gattcgaagg ccagcgccgg ctcctcgcgg tcgtcgtcgg
     2281 cacgacgggg ctcaggaagg gcctcgacgt cgagacggcc accgacgtcc tgtacgccct
     2341 cggcagcccc gagacctggc gactcctcgt ggtcgaccgc ggctggacgg cagacgaatt
     2401 cgaggactgg tacgccgaga cgctcgagcg gctgttcctc gaaccggatt cgaccgcgac
     2461 cgcgaccgcg gccgacgccg ccgatccgac gacctgatcg cctccggcgt ccgcaacggt
     2521 cgccgtcgac gttggtcgac gtgctccacc tagggccgaa tgcgctcgat cccgacgatc
     2581 ccgtcgaagc cggcgtcaaa ggtcaggatg cggctgatgt cacggcgctg catcaccgcg
     2641 agatgcaccg cgtcccgggc tgacaggctt gccgccgaac cgacgagacg ccgggcgcgg
     2701 gcgacgtcct cctgctcgat cgggtggacg atgtcgacca cgccgaggat cgcgtcccac
     2761 gccggtccga tggcgtcgcg acgactgatc gcggtgtacc gatgcagcaa ctcctggagc
     2821 acctcggcat cggtggaaag cggttcgttg tcgacgatcg cctgctcgag caggcgccgg
     2881 gcggcggtct tgttcggatg atccgcaccg acgagataca tcgggatgtt cgagtccacg
     2941 aagatcacgg actgtcagcc tgtccgtagc cgcgctcgat ctcgtcgagc atcacgccga
     3001 tctcggcggt cggaaacgag aacgccgcgg ccgcccgaac cgccgccagc ttctggccca
     3061 catccgcgcc ggcctcagcg tcgcgggccg cccgcaggga gcgccggacc cattccgcag
     3121 tcgtcatccg atgctgacgg gccagtcgct ggagttcccg gagctcgtcg tcctcgaaga
     3181 ggacctggag tcgtttggtc atgcgaagga cactaatgga ctcatcacca tgagtcaatg
     3241 acctcgcggg catcgaggcg cctcgagtgg caccggacgc gaaggagccc gggccggcac
     3301 cgaccgaccc gggctcgatc gcgaggaaga ggaggagagg ctacgcggcg gcggcgccga
     3361 ctcccacgaa gtcgtcgtac gcctcggccg cggcgacgac gacccgcgtc cgcccgccga
     3421 gcgcgagcgc cgcgagggcg ccgatcgcga cgaccaccgc tccgacctgc atcgccggga
     3481 cgagcccgtc gacgtaggtc tggccgctgg cgtacccgcc gttggccgag aagatcgagg
     3541 cgaggaccgc gacgccgaag acgccgccga gctcgcggat cgcgttgttc gcgccggatg
     3601 ccttgccctc ttcctcgggc cggacggccg agaggacgac gttggcgatc ggggcgaaga
     3661 agagacccat cccgatgccg gcgaggatga acgacgggac gatcgcgccg tagtcgacgg
     3721 tcggcgtgac gacgaatgac atccagccga gggcgatcgc ctggaggctc atcccgagga
     3781 cgagcagcgg gcgggtcccg gtccgggccg acaggagccc cgcgatcggg gccacgaaga
     3841 ccggcatgat cgtccagggc agggtccgca ggccggcctg gagcgggctg tagtgctgga
     3901 cgacctggaa gaactgggcg agcaggaaga tcgacccgaa catcccgaac gtcatcagca
     3961 tcgagacgac gttggcggcc gtgaacgaac gtgagcggaa gaggcggagg gggagcatcg
     4021 gctcggcgct ccgtgcctcc caggcaacga acgaggcgag gagcacgatc ccgaccgcga
     4081 atgagccgag gatctcgagg ctcgtccagc cctgttcgtt ggcccggacg agaccccaca
     4141 cgaccgcgag cagaccgccg ctgacgaacc cgaggccggg caggtcgaga cgggtcgagg
     4201 ggccgcgggt ctcgctcagg cgggcatagg ccatggggat cgcgatgagc ccgatcggga
     4261 cgttgatcca gaagattgcc tgccaggcga ggccctcgga gatcgcgccg ccgacgagcg
     4321 gcccgatcgc gatcgcgaga ccggcgatgc cgccccacgc gccgagggcg accgcccgtc
     4381 gctcgcgcgg gaccgcggcc gacaggatcg tcaggctcaa cggggtgagg atcgcgccac
     4441 cgagaccctg gacggcccgg gcgacgatca ggacggtgac gttcgaggag agcgcggcga
     4501 tcaccgagcc gcccgtgaag atggccagcc cgatgatgaa gagccgcttc cgcccgaatc
     4561 ggtcgcccag ggcggcgccg gtcaggagaa gcacggcaaa cgtcagggtg taggcgttga
     4621 cgatccattc gagctcggcg aggttggcgt tgaagctcgc ccggatcacg ggcagggcgg
     4681 tcgtcacgac gaggttgtcg agggcggcca tgaacagggc gatcgagctg atggcgaagg
     4741 tccagaggac ccggtggtcg ggtgccggac ggggtgcagc cacggacata ctccttgcta
     4801 gttagcagtt gctaagttca cgaacgaaaa agggggccgg gtggatttca tgccgctcgt
     4861 tcaggcgtcg ccctccatct gggcgaggat ccagctgtgc gggtcgccgg agaactcgac
     4921 ggcggccgcg atgttgagca gcatgccctc ggcgaagaag cggtggatgt ccatcggatc
     4981 ggcgccggac agctcccgga ccctccggtg caaccgcccg aattcctcgc gaacgacgac
     5041 ctggacgtcc ggatcggcac acgcggcgta ggcctggagc tggacgagca gctggttacg
     5101 atcggcgagg agcccgtggt aggacatgcc gatcgcgtcg aggaccgtgt tgcactggct
     5161 gttctcgccc gggacgaaat cgccggctgc ccgttcgaag gtcagccggg tccgctcgaa
     5221 gcagcgccgg acgaccgcca ggaacagctc cttcttcgtc ccgaacagct ggaagacgta
     5281 cggctgcgag accccggccc gacgggcgat cgcgtcggtc gaggcgccga ccagtccacc
     5341 tgtcgcgaac tcctcgaccc cggcggcgac gatcgcgtca cgccgctcgt cggcggtcat
     5401 gcggccgggc cgggtggtgg gaatggagcg ggtcggtgag gtcatgacac acaacttagt
     5461 aactactaag tcagttgtca agagcaatgt cgaacggggt cgccagcgaa cggcccgcgg
     5521 ctcgctcacc gacccgtcga tggtggtcgc gttcgcggtc gtcggcctga tcggcgcgct
     5581 cctcgcgatc cgtggcgtca ttccgctgcg atcggcggcg acgcgcgctg gcgccgcggc
     5641 aggggcgcgt tgaccgaggg cggtctggcg ggtcacgccc gcctcgtctc ggacccgggt
     5701 cacgccgctc gcgcttcgcc gacatctggt gggtgttcgg cgggctcgcg gtcgcgagcg
     5761 tcgtcggtgc cgtggtcgtt gccgtcaagg cgatccagca cctgtcgccg acttcgcgcg
     5821 actgacctcc tgcggtcggg ccggacaacc ggcccgaccc cgatcggtcc gggctgcatc
     5881 atggtccggt gcagaccgat cgactcttcc ggatcacggc gatcgcgtgg ctcgccacag
     5941 ccgtagcccg cgtcgccctg gccgccgtcc aggccgggac gcccggcccg ggtgtgtccc
     6001 ccgtcgcgtt tgcgatcggc gccggggcgg ggctgctcgt cgccgggctg ctgtggttcc
     6061 ggccgggtcg aggcagcgcg atcgcggcct gtgcgttcgg gctcttcgcg gtggtcggga
     6121 tcgcctacct cccgttcatc gggttccagc cgtggttcat cgtgctcagc ctggccgatc
     6181 tgatcgcgtt cgtgctgtcg gtcgtctgcc tcttcgccac gcgccgcccg ggccgttcaa
     6241 gcgacgcgga ccggtgggcg tgagcttgct cggcgggatc gctgaccgcg tcacggcgcg
     6301 gctgccgacg atggatcggc gcacggcgat cgtcctttcg ctcgcggtcg ttctcgtgcc
     6361 gctcgtgttc gtgctggtcg ttccgcaccc gctcgagcag ccgttcggcg tcgacttcca
     6421 gctctatcgc gacgtcacca ctcggtggct ggccggcggt tcgttcttcg agccgtacca
     6481 gctcgccggg ccgtacgaca tccgggccgg cgacgtcctg tacccgccgg tcgcgctgtg
     6541 gctgttcgtc ccgttcgcgg cggccggcgg cgtcctcgcc tcgctcgcgg cgatcggctg
     6601 gtggctggtc ccgatcggca tcacggcggc tgccgtcgtc cggctgcggc cgcgcccggc
     6661 cgtctggccg ttgatcgcgc tgtgcctcgc caacccgacg acgctgctca agatctggac
     6721 cggcaacccc gtcatgtggt cgatggcggc gatggccgtg g
//
LOCUS       DATAIW010000290         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_276083, whole genome shotgun sequence.
ACCESSION   DATAIW010000290 DATAIW010000000
VERSION     DATAIW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276083"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VHM48_09095"
     CDS             <1..226
                     /locus_tag="VHM48_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2755607.1"
                     /translation="LHIPEDLSVVGFDDLPVAQWVGPPLTTIRQPLIDMAMVAAEMVL
                     TIARGESPAQTRIELATELVIRESTAPPRE"
     gene            complement(284..1576)
                     /locus_tag="VHM48_09100"
     CDS             complement(284..1576)
                     /locus_tag="VHM48_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2755608.1"
                     /translation="MSGPLPDEALDALVLVLDEVRLGRSRSRSELVERTGLGRAIVSQ
                     RVGELIERGLVIEGDVGPSTGGRPPRQLTFRADAGHLLVADLGATSIDVALTTLDGRI
                     LGHRDEPAGIEAGPDVGLGRVEELFDQLVATTHAVPGRLWGIGVGVPGPVEFATGRPI
                     SPPIMPGWDGYPIRERFAARYGAPVWVDNDVNVLALGEWRSGVAAGHDDVVVIKIGTG
                     IGAGIISGGRLHRGAQGSAGDVGHIQVTDDPAVICRCGNIGCLEALAGGGAVGRSGEM
                     AAREGRSSRLRTALDQHGEVTAQDVARAASSGDPVAVVLLQDAGRRIGSMLASVVNFF
                     NPSLVVIGGGVANSPDQLLASIRETVYRRSLPLATRDLLIQHSSLGGLAGVIGASSMV
                     VDELFSRDAIGRWLASGEPSGMVEPVWHGTTTAASSNG"
     gene            1792..2997
                     /locus_tag="VHM48_09105"
     CDS             1792..2997
                     /locus_tag="VHM48_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2755609.1"
                     /translation="MSRFTDIGAAVIGTGFIGTVHVEQLRRIGVDVRGVLGSSPERGA
                     ARADALSVRHAYASLDEILSDPAVHVVHVTSPNHLHVPQATAILAAGKHVVCEKPLAM
                     TAGESAGLVTQARASGLVNAVNFNIRFYPLHQHMREVVAAGALGDVRFVTGHYFQDWL
                     LHDTDWNWRLEPDKGGTLRAVGDIGSHWLDLMAFVTGQPIVSVMADLATFVTARQEPT
                     GPVETFSTDRSANTVTRPMGTEDTATLLLRFANGARGSVAVSQISAGRKNSLQWEIDG
                     SESAAWWDSETPDHLWLGHRDRPNELLLRNPALMGPAGRTAAALPGGHVEGFGDTFGA
                     LFRAIYADVLTGRANPDPPYADFAAGHDEMLVNDAIGRSAAEERWVDVDRSAVGAISA
                     SPVLSEAAS"
     gene            3020..>3199
                     /locus_tag="VHM48_09110"
     CDS             3020..>3199
                     /locus_tag="VHM48_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755610.1"
                     /translation="MRLGLLTAPFPNTPLAEVVDWTAGNGFESIEIACWPRTSGPSRR
                     YAGTSHIDVANLSQSQ"
BASE COUNT          501 a         1171 c         1075 g          452 t
ORIGIN      
        1 gctccacatc cccgaggatc tcagcgtggt cggcttcgat gacctgccag ttgcccaatg
       61 ggtgggaccg ccgctgacga cgatccgtca gccgttgatc gacatggcaa tggtcgcggc
      121 cgagatggtc ctcaccatcg cgcgcgggga gtccccggcg cagacgcgga tcgaattggc
      181 aacggagctc gtgatccggg agagcaccgc tccaccgagg gaatgacgcg accgggtcga
      241 cggggtccgc cgcgcgttca cgcgctggcc gtcgaacgcg gagtcagccg ttcgacgacg
      301 ccgcggtcgt cgtcccgtgc cagaccggct cgaccatgcc gctcggctcc ccggaggcga
      361 gccaccggcc gatggcatcg cgcgagaaca gctcgtcgac gaccatcgat gaggcgccga
      421 tcacgccggc caggccgcca agcgatgagt gctggatgag caggtcacgc gtcgccaacg
      481 gcaacgagcg gcgatagacg gtctcgcgga tcgaggcgag cagctgatcg gggctgttgg
      541 cgacgccgcc gccgatcacg accagcgacg ggttgaagaa gttcacgaca ctcgcgagca
      601 tcgagccgat ccgccggccg gcgtcctgca gcaggacgac cgcgacggga tcgcccgacg
      661 aggccgcccg ggcgacatcc tgggccgtca cttcaccgtg ctggtcgagc gccgtccgga
      721 gccgggacga tcgcccttcg cgggccgcca tctcacccga ccggccgacc gcgccaccgc
      781 ccgcgagcgc ctcgaggcag ccgatgttgc cgcagcggca gatcacggcc ggatcgtcgg
      841 tgacctggat gtggccgacg tcgccggcgc tcccctgggc gccgcggtgg aggcgaccgc
      901 cgctgatgat cccggccccg atgcccgtcc cgatcttgat gacgacaacg tcatcgtggc
      961 cggccgcgac gcccgaccgc cactcgccga gcgccaggac attgacgtcg ttgtcgaccc
     1021 agacaggggc gccgtagcgg gccgcgaacc gctcgcggat cgggtagcca tcccagccgg
     1081 gcatgatcgg cggggagatc gggcggccgg tggcgaactc gaccggaccc gggacgccga
     1141 cgccgattcc ccacagccga cccggcacgg cgtgggtcgt cgcgacgagc tggtcaaata
     1201 gctcttcgac gcggccgaga ccgacgtccg ggcccgcttc gatcccggcc ggctcgtcgc
     1261 ggtgaccgag gattcggccg tcgagcgtgg tcagggcgac atcgatgctg gtggctccga
     1321 ggtcggcgac caggaggtgc ccggcatccg cccggaacgt cagttgccgt ggcggccggc
     1381 cgcccgtcga gggacccacg tcaccctcga tgacgagtcc gcgctcgatc agctcgccga
     1441 cccgttgcga gacgatggcc cgcccgagac ccgtccgctc gacgagctcg gagcgggatc
     1501 tcgaccgacc gagccggacc tcgtcgagga cgaggaccag ggcgtcgagg gcttcgtcgg
     1561 gcagcggacc ggacatggac ccgatgatac ggcccggacg tgcgcccgga cgtgcgccga
     1621 gccacttttg cgcttgacga tccgaagtac gatgatgagc gttcgaacga tcgagcgcga
     1681 agtctcttga ccttgccgga ttcgactggc taccatcccg ttgcgctcta gaacgtctag
     1741 acaactcgcc ggaccctccc ggcacggcac ccggccaccg tcaggagctg gatgagcagg
     1801 ttcaccgaca tcggtgccgc cgtcatcggc accggattca tcgggaccgt ccacgtcgag
     1861 cagctgcgcc ggatcggcgt cgatgtccgc ggcgtcctcg gcagcagtcc ggaacgcggc
     1921 gccgcccggg ccgacgcgct cagcgtccgc cacgcctacg cgtcactcga cgagatcctc
     1981 tcggacccgg ctgtccacgt cgtccacgtg acctcgccga accatctcca cgtcccccag
     2041 gcaacagcga tcctcgcggc cggcaagcat gtcgtctgcg agaagccgct ggcgatgacc
     2101 gccggggagt ccgctgggct cgtcacgcag gcgcgcgcga gcggcctcgt caacgccgtc
     2161 aacttcaaca tccgcttcta tccactccat caacacatgc gcgaagtcgt cgcggccggc
     2221 gcgctcggcg acgttcgctt cgtcaccggc cactacttcc aggactggct cctccacgac
     2281 accgactgga actggcgcct cgagcccgac aagggcggca cgctccgggc ggtcggcgac
     2341 atcggctcgc actggctcga tctcatggcg ttcgtgactg gtcagccgat cgtctcggtg
     2401 atggctgacc tcgcgacgtt cgtgacggcc cgtcaggagc cgaccggacc ggtcgagacc
     2461 ttctcgaccg atcgatctgc gaacacggtc acccggccga tgggaaccga ggacacggcg
     2521 acgctcctcc tccgctttgc caacggtgcc cgcggctcgg tggccgtgtc gcagatcagc
     2581 gccggccgaa agaactcgct ccagtgggag atcgacgggt ccgagagcgc ggcgtggtgg
     2641 gactcggaga cgcccgacca tctctggctc ggccaccgcg accgaccgaa cgagctgctc
     2701 ctgcgcaacc cggcgctgat gggcccggcg ggtcgcacgg cggccgccct tcccggcggc
     2761 cacgtcgagg gcttcggcga cacgttcggc gccctcttcc gggcgatcta cgccgacgtc
     2821 ctcaccggcc gcgccaaccc ggacccgcca tacgccgact tcgccgccgg ccacgacgag
     2881 atgctcgtca acgacgcgat cggccgcagt gccgccgagg agcgctgggt cgatgtcgac
     2941 cggtccgccg tcggcgcgat ctccgccagc cccgtcctct cggaggcggc ttcatgagcg
     3001 tcccatcgga ggtcctttcg tgagactcgg cctgctgacc gccccgttcc cgaacacgcc
     3061 gctcgccgag gtcgtcgact ggacagccgg gaacggcttc gagtcgatcg agatcgcctg
     3121 ctggccgcgg acgtcgggac cgagccgccg ctatgccggg acgtctcaca tcgacgtcgc
     3181 caatctctcg cagagccag
//
LOCUS       DATAIW010000291         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_18121, whole genome shotgun sequence.
ACCESSION   DATAIW010000291 DATAIW010000000
VERSION     DATAIW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18121"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VHM48_09115"
     CDS             complement(<1..201)
                     /locus_tag="VHM48_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755611.1"
                     /translation="MSDPPTLRIVAERPTPGTPRPYDFPAVERTRLANGLSLAVIHLP
                     GRPLVAGTLVLRNGAADEPDGEG"
     gene            complement(198..>1523)
                     /locus_tag="VHM48_09120"
     CDS             complement(198..>1523)
                     /locus_tag="VHM48_09120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2755612.1"
                     /translation="LRLIIAVDRLAPVVAVNLWYDVGSKHEVKGKTGFAHLFEHVMFQ
                     GSRNVGKAEHMAFVQGAGGTMNGSTWLDRTNYYETLPSHQAELALWLEADRMGTLLDA
                     LNQENLDNQREVVKNEKRWSYDNRPYGSWYEKIQGHLYPPDHPYHHTTIGSMEDLDAA
                     SIEDVSAFFRTYYAPNNAVLSVVGDVEPDEIRRWTERYFGGIPANPDIPRLGDLSLPP
                     ILGAEVRETVHDRVPLPRVYVGFRAPAFGDDRLDALDIAAQVLAGGKGSRLHRRLVRD
                     ERIAQDVALVALGFVGGASICVGWATVRPGIPVERVEAALHEELDRLAREPISDDELA
                     RARALIETEELGGLSRVEERADRLSMYATLFDDPDLINQMLPRYLAITADQIQEVAAA
                     TFRTDNRVVLTYLPELPPAETATNDAEAPGDPPDVASEDDAADDEEVAA"
BASE COUNT          206 a          501 c          570 g          246 t
ORIGIN      
        1 gccttcgccg tcgggctcgt cggctgcgcc gttgcggagg acgagcgttc ctgcgacgag
       61 cggccgaccg gggaggtgga tgacggccag cgagagtcca ttggcgaggc gcgtccgctc
      121 gaccgccggg aagtcgtacg gccgcggcgt gcccggtgtc ggccgttcgg ccacgattcg
      181 cagcgtgggc ggatcgctca tgccgcgact tcctcgtcgt cggccgcgtc gtcttcggag
      241 gcgacatcgg gcggatcgcc gggtgcctcg gcgtcgttgg tcgcggtctc ggccgggggc
      301 agctcgggca ggtaggtcag gacgacccgg ttgtccgtcc ggaaggtggc ggcggcgacc
      361 tcctggatct ggtcggccgt gatggccaga tagcgcggca gcatctggtt gatcaggtcc
      421 gggtcatcga agagggtcgc gtacatcgag aggcggtcgg cccgttcctc gacccgcgac
      481 agaccgccga gctcctcggt ctcgatcagg gcccgtgccc gcgccagctc gtcgtcgctg
      541 atgggttcgc gcgcgaggcg gtcgagctcc tcgtggaggg cggcctcgac ccgttcgacc
      601 ggaatgcctg gccggacggt cgcccaaccg acgcagatcg acgccccgcc gacgaacccg
      661 agcgcgacga gggccacgtc ctgggcgatc cgctcgtcgc ggacgaggcg gcgatggagg
      721 cgcgacccct tgccgccggc caggacctgg gcggcgatgt cgagggcgtc gagccggtcg
      781 tcgccgaacg ccggcgcccg gaacccgaca tagacccgcg gcaacggcac gcggtcgtgg
      841 acggtctcgc ggacctccgc accgaggatc ggcggcagcg acaggtcgcc cagccggggg
      901 atgtccggat tggccgggat gccgccgaag tagcgctcgg tccagcgccg gatctcgtcg
      961 ggctcgacgt cgccgacgac cgacaggacg gcgttgttcg gggcgtagta cgtgcggaag
     1021 aacgcgctga cgtcctcgat cgaggccgcg tcgagatcct ccatcgagcc gatcgtggtg
     1081 tggtgatagg gatggtcggg cgggtagagg tggccctgga tcttctcgta ccacgagccg
     1141 tacggccggt tgtcgtacga ccagcgcttc tcgttcttca cgacctcgcg ctggttgtcg
     1201 aggttctcct ggttgagggc gtcgaggagg gtccccatcc ggtccgcctc gagccacagg
     1261 gcgagctcgg cctgatggga cggcagggtc tcgtagtagt tcgtccggtc gagccacgtg
     1321 ctgccgttca tcgtcccgcc ggcgccctgg acgaaggcca tgtgctcggc cttgccgacg
     1381 ttccgcgacc cctggaacat cacgtgctcg aagaggtggg cgaagccggt cttgcccttg
     1441 acctcgtgct tggatccgac gtcgtaccag aggttgaccg ccaccaccgg ggcgagccga
     1501 tcgaccgcga tgatcaggcg cag
//
LOCUS       DATAIW010000292         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_18226, whole genome shotgun sequence.
ACCESSION   DATAIW010000292 DATAIW010000000
VERSION     DATAIW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18226"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1575
                     /locus_tag="VHM48_09125"
     CDS             400..1575
                     /locus_tag="VHM48_09125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX2755613.1"
                     /translation="MLRLLHTADVHLGARHTDLGEQAAAQRERQFAAFRAAVDLAIGE
                     KVDIVLIAGDLFDSNTQPKRSVERVAAELKRLVEAKIRTVIIPGTHDVYDRSSLYRVH
                     DLAVLAGSGEDDDLVTVLTPDRPSIHLGACDVVVHGPVFGTKRAPHSPLRDLRVVAGD
                     DATWHVAMVHGSLSIPDRTDRDEVVFTREEIEATGLDYLALGHWHSAQQGKAGATTWA
                     YSGAPEPVSVTQDGAGKVLLVELNEANGKRTVTIAERVVGRTRFEKLDVDASAVADQP
                     ALVAAIAARADPDVVLDARLTGVRPDELDLSVDEIEEQLKGSFLKLRVRDASMPALTQ
                     GNLPSPDTIPGAFIRNVEGRIAELEADGTDQSSVEAAELRDVLRLGRLLLAGHEVTL"
     gene            1572..3779
                     /locus_tag="VHM48_09130"
     CDS             1572..3779
                     /locus_tag="VHM48_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755614.1"
                     /translation="MRITRLTLRDFRRYRELEIPLSPGLTIVHGPNEAGKTTIQRAIE
                     LALTRKVTSGGADMDALRSWDAGDDARPVIGLEFEIEDEERGVRTGTLEKEFRGPKGT
                     VRLEVDGTTITDPTLADQAIAEMTGIPSEAFFRSTASVRHHELADLDRDEAALRDRLQ
                     ASISGADRGTSRARRKLEKAIYDLNTKGAKNPGRLKVAEDAVEQARLAVEQGELALAQ
                     LERDRDTLSGARERRSETETSLSERRSLLEKARQAERLTADRVGAQERFERYRQAVAV
                     SEELAGLHASHPSVNPLPVLEQVIGRLRSLDAKIRELRAALSGEVEVSFDVPPEPTWR
                     PLSRIALVLVVVGVLVAGVTVGARALNVFDIGTTPELLGGAAAGIGFILAFVALWLRR
                     SYRMQAEMRDVEIDRRLRGRSQMEQELRQAETDTAQQLAGLGHEDLAAAEDLLAREQE
                     HVGRIDRLAAQLDGLVGKEPADTLPTVRDAAALEVEQKTHALEALGPIAKEPRARERL
                     EVEVRDLESGLERARDDEANARARVEANAIDAEQVAGHSERLSGWREQLGALQRRARV
                     YDRTLKEIESAEQATMKTATRFLEKQMVGDVERITGGRYRRVAVDDRTLDIQLHAPEK
                     GDWVDVRSLSQGTLDLVYLAARLGLVRLVTGDRRPPLIFDDPFVTLDDDRARRALDLL
                     REIAKDFQVIYLTTSDRYHASADAVVALAGPTEVDDGSVEAGAAVAGAGAVHG"
     gene            3724..4644
                     /locus_tag="VHM48_09135"
     CDS             3724..4644
                     /locus_tag="VHM48_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2755615.1"
                     /translation="MDRSRPAPRSRAPELSTADPAGPGLAVVVLGLASAIAWGAGDFG
                     GGWTGRRAPVLGIAIGIDLIGTVLMVGVALATGEPMPGLATLGLAALAGVLAVAGILG
                     LYTGLAVGRMGVVAPVTGVIAATLPVVVGFVGLGLPRAEVVVGIALALGAVVLVSRST
                     DHSGRRSGIEYALLGGIGLGLFNIAVGAFPEHRVAWPLAVIKAGSLIPIVAIVVLGRR
                     PWRIPRPIVPALLATAGLDLAGNGLYILATQAGRLDIAATLSSLYPVTTVVLAVILLH
                     EQVTRSHLFGIMMAALAIALIAGGSAAPVA"
     gene            4663..4968
                     /locus_tag="VHM48_09140"
     CDS             4663..4968
                     /locus_tag="VHM48_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2755616.1"
                     /translation="MTEPSAPSPIELVWLVEATYAPDAAETRVPFRAEHIARLQDLKR
                     QGIVIEAGAFTDVSATLVIVRAADEEAALAVCRDDVYLRNGVWVELRARSFGRVRDD"
     gene            5069..>5542
                     /locus_tag="VHM48_09145"
     CDS             5069..>5542
                     /locus_tag="VHM48_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755617.1"
                     /translation="MRLPTFSAAVLARLLVLGGALILVGSLLSWVVFSVQPLFGWTYR
                     NGLDLGFGIVTALAGIGILTLGARLRVERPLSAWTAVGLALVTLALVALALVVAMNHD
                     PSDGRMFDGIAPGAIVVAFGAMLAALAGWRFAIRSSAAREPGSSPRRPRWQGAALL"
BASE COUNT          771 a         2000 c         1935 g          836 t
ORIGIN      
        1 ctggcacgtt ggctggtccc gggcgaaacc ggggcggcca tgtcggcgga cgggatggtg
       61 cggttgcgtc ggaaggggcc ggcgccgcgc tatccaggtg ccgtgatgga gtccctgtct
      121 cggggcgcac ggatttgatc gatggctcga ccatacaccg atcttccgga tcgggcgttg
      181 caacagccgg gacagcagcg atcagggaga cggcgatccg gccagtgtcg cgactgcccc
      241 cgcgtctggc acaatgcgtc gaacagtcga ggcggcgagc gggagttcgg gggcggcctt
      301 gcccggtacc ggcgccgggg atgaggacgg tccctggact gggcgagcca tctcgtctgg
      361 tcgcccagcc gcgacggcac aggaggccag cccgaccgga tgcttcgact cctccacacc
      421 gcagacgtcc atctcggcgc acgccatacc gacctcggcg agcaggccgc cgcccagcgc
      481 gagcggcagt tcgcggcatt cagggccgcg gtcgatctcg cgatcggcga gaaggtcgac
      541 atcgtcctga tcgccggcga ccttttcgac tcgaacacgc agccgaagcg gtcggtcgag
      601 cgggtcgccg ccgagctgaa gcggctcgtc gaagcgaaga tccggacggt catcatcccc
      661 ggcacccacg acgtctacga ccgctcgtcc ctgtatcgcg tccacgacct ggcggtgctt
      721 gccggctccg gcgaagacga cgatctcgtg acggtcctga cccctgatcg gccgagcatc
      781 cacctcggcg cctgcgacgt cgtcgtccac ggtcccgtct tcggcacgaa gcgagcgccg
      841 cacagcccgc tccgcgacct tcgcgtcgtc gccggcgacg acgcgacctg gcacgtggcg
      901 atggtccatg gctcgctgtc gatccccgat cggaccgacc gcgacgaggt cgtcttcacc
      961 cgcgaggaga tcgaggcgac cgggctcgac tacctcgccc tcgggcactg gcactccgcc
     1021 cagcagggca aggccggggc cacgacgtgg gcatactcgg gcgctcccga accagtgtcg
     1081 gtgacccagg acggggcggg caaggtcctc ctcgtcgagc tgaacgaggc caacggcaag
     1141 cggaccgtga ccatcgccga gcgcgtggtc ggccggaccc gcttcgagaa gctcgatgtc
     1201 gacgcctccg ccgtcgcgga ccagcccgcg ctcgtcgccg cgatcgccgc cagggccgac
     1261 cccgatgtcg tgctcgatgc ccgcctcacc ggtgtccgcc ccgacgagct cgacctgtcg
     1321 gtcgacgaga tcgaggagca gctcaagggc agcttcctga agctccgcgt ccgcgacgcg
     1381 tcgatgccgg ccctgaccca gggcaacctg ccgtcaccgg acacgatccc gggcgcgttc
     1441 atccggaacg tggagggccg gatcgcggag ctcgaggccg atgggacgga tcaatcctcc
     1501 gtcgaggccg ccgagcttcg tgacgttctc cggctcggcc ggctcctgct cgccggtcac
     1561 gaggtcacgc tgtgaggatc acccggctga ccctccgcga cttccgccgc taccgcgagc
     1621 tggagatccc gctgtcgccg ggcctgacga tcgtccacgg gccgaacgag gccggcaaga
     1681 cgacgatcca gcgggcgatc gagctggccc tgacccgcaa ggtgaccagc ggcggcgccg
     1741 acatggatgc cctccgttcg tgggacgccg gggacgatgc gcggcccgtc atcggcctcg
     1801 agttcgagat cgaagacgag gagcgcggcg tccggaccgg gacgctcgag aaggagttcc
     1861 gcgggccgaa gggaacggtg cgcctcgagg tcgacgggac gacgatcacc gacccgacgc
     1921 tcgccgatca ggccatcgcc gagatgaccg gcatcccgag cgaggcgttc ttccgctcga
     1981 cggcctcggt ccgccaccat gagctcgccg atctcgatcg cgacgaggcc gccctccgcg
     2041 accgactcca ggcctcgatc agcggcgccg accgcggcac ctcgcgggcc cgccgcaagc
     2101 tcgagaaggc gatctacgac ctcaacacga agggcgccaa gaaccccggc cgcctcaagg
     2161 tcgccgagga cgccgtcgag caggcaagac tcgcggtcga gcagggcgag ctcgccctgg
     2221 cccagctcga acgcgatcgc gacaccctct cgggtgctcg cgaacggcgg tctgagaccg
     2281 agacgagcct gtccgaacgg cggtcgctcc tcgagaaggc tcgtcaggcc gagcgcctga
     2341 ccgcggatcg ggtcggcgcc caggagcgct tcgagcgcta ccgccaggct gtcgcggtct
     2401 cggaggagct cgccggcctc catgccagcc acccctcggt caacccgctg cccgtcctcg
     2461 agcaggtgat cggccggttg cggtcgctcg atgcgaagat ccgcgagttg cgcgcggccc
     2521 tgtccggtga ggtcgaggtc tcgttcgacg tcccgcccga gccgacctgg cgcccgctgt
     2581 cgcgcatcgc cctcgtgctc gtcgtcgtcg gcgtcctcgt ggccggggtg actgtcggtg
     2641 cgcgtgcgct caacgtcttc gatatcggca cgactccgga gctgctcggc ggcgccgccg
     2701 ccgggatcgg cttcatcctc gcgttcgtcg ccctctggct gcgccgcagc taccggatgc
     2761 aggcggagat gcgcgacgtc gagatcgatc gccggctgcg cggccgatcc cagatggagc
     2821 aggagctccg acaggccgag acggacaccg cccagcagct cgccgggctc ggccacgagg
     2881 atcttgcggc cgccgaggac ctgctcgccc gcgagcagga gcacgtcggc cggatcgatc
     2941 gcctggcggc ccagctcgat gggcttgtcg gcaaggagcc ggccgatacc ctgccaacgg
     3001 ttcgcgacgc ggccgctctc gaggtcgaac agaagacgca cgccctcgag gcgctcgggc
     3061 cgatcgccaa ggagccgcgc gcccgcgagc gcctcgaggt cgaggtccgc gatctcgagt
     3121 ccgggctgga acgcgcccgc gatgacgagg cgaacgcccg cgcccgggtc gaggccaacg
     3181 cgatcgacgc cgagcaggtc gccggtcaca gcgagcggct cagcggctgg cgagagcagc
     3241 tcggcgcact ccaacgacga gcccgggtct acgaccggac gctcaaggag atcgagtcgg
     3301 ccgagcaggc gacgatgaag accgcgaccc gcttcctcga gaagcagatg gtcggcgacg
     3361 tcgagcggat caccggtggc cggtaccgtc gggtggccgt ggacgatcgg accctggaca
     3421 tccagctcca cgcccccgag aagggcgact gggtcgacgt ccgatcgctc agccagggca
     3481 ccctcgacct cgtctatctc gccgcgaggc tcggcctcgt ccgcctcgtg accggcgatc
     3541 gccgtccgcc gctgatcttc gacgatccgt tcgtcaccct cgatgacgat cgagcccgac
     3601 gggccctcga tctcctccgc gagatcgcga aggacttcca ggtcatctac ctcacgacat
     3661 ccgatcgcta ccacgcctcg gccgacgcgg tcgtcgccct cgccggtccg accgaggtcg
     3721 acgatggatc ggtcgaggcc ggcgccgcgg tcgcgggcgc cggagctgtc cacggctgat
     3781 ccggccggcc cgggcctcgc cgtcgtcgtc ctcggactcg cctcggccat cgcctggggt
     3841 gccggcgact tcggtggggg atggacgggc cggcgcgcac cggtcctcgg gatcgcgatc
     3901 gggatcgacc tgatcgggac ggtcctgatg gtcggggtgg cgctcgcgac cggtgagccg
     3961 atgccgggcc tcgccacgct cggtctggcc gccctggccg gcgttctggc ggtcgctgga
     4021 atcctcgggc tgtacaccgg cctcgccgtc gggcggatgg gggtggtcgc tccggtcacc
     4081 ggcgtgatcg cggccacgtt gccggtcgtc gtcggcttcg tcggcctcgg gttgccacgc
     4141 gccgaggtcg tggtcgggat cgccctcgcc ctcggcgcgg tcgtcctcgt ctcgcgctcg
     4201 accgatcatt ccggccggcg gtcggggatc gagtacgcgc tgctcggcgg gatcgggctc
     4261 gggctgttca acatcgcggt cggggcgttc cccgagcacc gcgtcgcctg gccgctggcg
     4321 gtgatcaagg ccggatcgct gatcccgatc gtcgcgatcg tggtcctcgg ccggcggccg
     4381 tggcggatcc cgcggccgat cgtccccgcc ctgctcgcga cggccggcct ggacctcgcg
     4441 ggcaacggcc tgtacatcct cgccacccag gcgggtcggc tcgacatcgc ggccaccctg
     4501 agctcgctct acccggtgac gaccgtggtc ctcgcggtca tcctgctcca cgagcaggtg
     4561 acgcggagcc acctcttcgg gatcatgatg gccgcgctcg cgatcgccct gatcgcgggg
     4621 ggatcggccg cgccagtcgc ctgaccacca gcggagaccg ccatgaccga gcccagcgcg
     4681 ccttctccca tcgagctcgt ctggctggtc gaggcgacct atgccccgga cgcggccgag
     4741 acccgcgtgc cgttccgggc cgagcacatc gcccggttgc aggatctcaa gcggcagggg
     4801 atcgtcatcg aggccggcgc cttcacggac gtcagcgcga cgctcgtcat cgtccgcgcc
     4861 gccgacgagg aggcggccct cgccgtgtgc cgcgacgacg tctatctccg caacggcgtc
     4921 tgggtggagt tgcgggcccg ctcgttcgga cgggtcaggg acgactgagt tcggcagcaa
     4981 ggtgagaatc cggttggtgg gtaaggctct cgcccacctg cggcccccag cacttgtccg
     5041 tgccacgcca tggaccgatg ctggacgggt gagactaccg acgttcagcg ccgccgtgct
     5101 tgcccgcctt cttgtcctgg gcggcgcatt gatcctggtc ggctcgctgc tgagttgggt
     5161 cgtgttctca gttcagccgc tgttcggctg gacctaccgc aacgggctcg atctcgggtt
     5221 cggcatcgtg accgcgcttg ccgggatcgg catcctgacc ttgggtgctc gcttgcgcgt
     5281 cgagcgcccg ctgtcggcct ggaccgctgt cggcctcgcc cttgtcacgc tcgctctcgt
     5341 cgcgctggca ctcgtcgtcg caatgaacca cgatccaagc gacgggcgaa tgttcgacgg
     5401 gatcgccccc ggcgcgatcg tcgttgcctt cggcgcgatg ttggctgctc tcgcaggctg
     5461 gcggtttgcg atccggtcaa gcgcagcgcg tgagcctggc tccagtccga ggcggccgag
     5521 gtggcagggc gctgccctgc tc
//
LOCUS       DATAIW010000293         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_78470, whole genome shotgun sequence.
ACCESSION   DATAIW010000293 DATAIW010000000
VERSION     DATAIW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78470"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VHM48_09150"
     CDS             complement(<1..1113)
                     /locus_tag="VHM48_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755618.1"
                     /translation="MHDRTVVPIDPRLVVNGPLDPSLDAIRVGLAGHRRRLWMRRIVR
                     RAWLALAGIGVLEAALLGLARLVPIEILPSLVAAIAIGGLMVLLAFAVRARPSLGETA
                     VAVDGEGRLGDRVSSALSLATTYPALAGPADPAAEDTAGPISDAGEAERFVRRQRLDA
                     IGALRVAPPNLFRARLSRQPAAIALAATLLIVPFALLPNTQDRVIAEARANRDEAVKQ
                     ASRIEEVAKELESKGARPDDPRTRLATDLRELAAQLRAHPEDLKANLAKLGSVEASLH
                     SQLDPGNEQRASALTSLNRSLSRTASGDPNANKDGDPKKTADDLKNVGSKLDQMTPEQ
                     RSAMAAALAGLQSDASQASGAAGQALRDAAQAISQGQ"
     gene            complement(1191..2513)
                     /locus_tag="VHM48_09155"
     CDS             complement(1191..2513)
                     /locus_tag="VHM48_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2755619.1"
                     /translation="MTDERVTPPAEEAPQPGPMIEETTVDSAAPGAVRTESVGAHSAR
                     PRRPRPNPVRAFRSGVDGVIAVGVKELRGRVRGRRAFVVLTFYLLFLALFAWAWELRE
                     EVVYVQNAALQGGSATLASSLVGQEVFGALVLVETLLVVFLAPAFTAGAISLEREKQT
                     LDMLTATPISSLAIVVGKLLSALAYVFLLIAASIPLTAIVFVFGGVAPDDVLRAYVVL
                     IVTALGLGSMGLFISAIMQRTQGATVVTFFTVLFLTLGTMFGVLFWNTMAGVNSNTGF
                     VTGSSGVGPIKGRAPEAFLWFNPFAAQYDVICGASDGYGGWCSRLNTITNGAITVGGS
                     GTGGGIFPTTPSQPGFGFDGGGTGVVTLPAVGGAVPAPDFQGKVNVGIGGFGATDPQP
                     FGIRRDTFWPRSLQAWGVMSVLFILASIQLVSPTRRWRVLRRPFRRAA"
     gene            complement(2515..3474)
                     /locus_tag="VHM48_09160"
     CDS             complement(2515..3474)
                     /locus_tag="VHM48_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755620.1"
                     /translation="MTDIVVTRGLVKRYPGTLAVAGLDLAVGEGEIFGLVGPNGAGKT
                     TTLRILATLLSATSGHAEIAGIDVRRNPDAARRVLGFMPDVFGVYDDMKVWEYLDFFA
                     RCYGIPASRRRRMIGDLLDLVDLGEKRDAYVQGLSRGMQQRLCLAHTLVHDPEVLLLD
                     EPASGLDPRARVELRELLRELRALGKTIVISSHILPELEELCTAVAIVDHGQVLAHGK
                     VSEIERRLRFGAVLRVRILTGHSREDARNHFAADPRVASATILDDGQIELGFRGDDEA
                     SAQLLAAAVAAGIRITSFARAASDLEELFLQVTATAGQQTQVA"
     gene            complement(3471..5969)
                     /locus_tag="VHM48_09165"
     CDS             complement(3471..5969)
                     /locus_tag="VHM48_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755621.1"
                     /translation="MTRHAPRRILRLVVLLATASLLVVAALGPAAAGAVAADPPTMTA
                     RVLLQGHARLGSWIAIEVHLVNSGPSISGEIRLQGGSQGGTRYATAVQLDSPSDKTWV
                     LHAQPPSFGQQLEVVLVGGGQILLRQKVAVTIHDPGQLVVGVVAEAAPKIVGSLSLPA
                     VQNQQPAVVVPLTVADLPTRIEAWSALDRLIWQDVDASTLSKEQLTALRGWLALGGRL
                     VIVGGTGGIGSLGGFPDDILPYRPTSTVDVAAASLISLLGTPPKDAGDVPAMAGDLIR
                     GRALATSGDRVVAAQTPYGSGSVTILGIDPTVGWVAQATASRSLWPSLIPARSDGSVG
                     MTDDSQIVSAVSNLPSLALPPLGGLLLLLFGYIALIGPLNYLILWRLDRREWAWITMP
                     ILIVGFAAGAYAFGSALRGSSIIVNEVGIVRGAPDATEGSAQVYLGVFSPTRGTYQVS
                     VPGGALLSSPISGDVFGGTTASLDIVQGDPPGPSRVRDLSVGFGSLRTIRAESQAVVP
                     KVHAELALVDGTLTGTIRNDSAVKLEKPAIVLGSNVKVLKDLQPGEQVQVSLAITNQN
                     AFGQSLSDRIFGQIFFGQTGATTETARRDQTRHLVVDQLTYDPQFGNFGRLSSDGPVL
                     LAWGRDAVMDVAVEGQATNRVSNVLYYVPLAMGVHGSVAFSGDLVKSTMTETDAAFFN
                     KDPMTLSFGQGSVTMVYRPIAFDGTLTATHVRLGVSSGPDGSVNGVSGVKVAPIPDAC
                     LNVKAPAKPPANCPKALPADQFDGMPEVELFDRTGDGTWHRLPHLSQGQTYDVADAAK
                     YVDPSTGAVLVRFVNERQDPVNVYLNLSIEGTVK"
     gene            6207..>7115
                     /locus_tag="VHM48_09170"
     CDS             6207..>7115
                     /locus_tag="VHM48_09170"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX2755622.1"
                     /translation="MPQIRPFRALRYAPDSVGDVAAVVAPPYDVLTAADRARLLARHP
                     KNVVRLDAPTDEAGDAEDDRYRRAARTLAAWRSDGTLHKDPRPAIYVHEQTYLVPGTD
                     VERTQRGFFARLRLEALVPPAGVLPHERTLAGPREDRYRLLRATGVNTSPVIGLYDDP
                     GGHAAAILATLATGTAAIDVVDDDGVRHRVWIVDADGPASNAVSELVAIAAADPVTIA
                     DGHHRYETALRYRDERRMSRSCEEDPAFDYLLMLFLETNAEPLTILSTHRLVAIAQGC
                     LVAMVAIGDRHRVRGRDRHQLGHGVRS"
BASE COUNT         1097 a         2444 c         2595 g          979 t
ORIGIN      
        1 ctgaccctgc gagatcgcct gcgctgcatc gcgcagcgcc tgtccggcgg ccccgctcgc
       61 ctgcgaggca tccgactgga ggcccgccag cgcggcggcc atcgcgctcc gctgctcggg
      121 ggtcatctgg tccagcttgc tgccgacgtt cttcaggtcg tcggccgtct tcttcgggtc
      181 gccgtccttg ttcgcgttgg ggtcgcccga tgccgtccgc gacagcgagc ggttgagtga
      241 cgtcaaggcc gacgcccgct gctcgttgcc gggatcgagc tgcgaatgga gcgacgcctc
      301 gacagagccg agcttggcga ggttggcctt gaggtcctcc ggatgagctc gcaactgagc
      361 cgcaagttcg cgcaggtcgg tcgcaaggcg ggtccgcgga tcgtcgggcc tggcgccctt
      421 cgactcgagc tccttggcga cctcctcgat ccggctcgcc tgcttgaccg cttcgtcacg
      481 gttggcgcgg gcctcggcga tgacccgatc ctgtgtgttc ggaagcagcg cgaacggcac
      541 gatcaggagg gtcgccgcga gggcgatcgc cgcgggctgg cgggaaagcc gcgcccggaa
      601 caggttcggg ggcgcgacac gaagggcgcc gatcgcgtcg agccgctgac gccggacgaa
      661 ccgctcggct tcgccggcat cgctgatcgg tccggccgtg tcctccgccg cgggatccgc
      721 cggcccggcg agggctgggt aggtggtcgc gagcgagagg gcgctcgaga cgcggtcgcc
      781 gagacggccc tcgccgtcga cggccacggc cgtctcgccg aggctcggtc gtgcccggac
      841 ggcgaacgcg agcaagacca tcaggccgcc gatcgcgatc gccgcgacga gggaggggag
      901 gatctcgatg ggaacgagcc gagcgagacc gagcagtgcg gcctccagga cgccgatccc
      961 cgccagggcg agccaggccc ggcggacgat ccgccgcatc cagaggcggc ggcgatggcc
     1021 ggccagaccg acgcggatgg cgtcgagcga cggatcgagg ggcccgttca cgacgagccg
     1081 cgggtcgatc ggaacgacgg ttcggtcgtg catcggcccc gcccctcgac gccctgcggc
     1141 gcggagtccc cggccgagcc gtcccgagcg aatcgagcga ggtgacggca tcaggcggcc
     1201 ctccggaacg gccgccgcag cacgcgccag cgacgggtgg gggagacgag ctggatcgag
     1261 gcaaggatga agaggaccga catcacgccc cacgcctgga ggcttcgcgg ccagaacgtg
     1321 tcgcgcctga tgccgaacgg ctgcggatcg gtcgccccga acccgccgat gccgacgttg
     1381 accttgccct ggaagtcggg ggcaggcacg gccccaccaa ccgccggaag ggtgacgaca
     1441 ccagtgccgc caccgtcgaa tccgaacccg ggctggctcg gggtcgtcgg gaagatcccg
     1501 cccccggtcc cggagcctcc gacggtgatc gccccgttcg tgatcgtgtt caggcgactg
     1561 caccagccgc cgtacccgtc ggacgcgccg cagatgacgt cgtactgggc ggcgaacggg
     1621 ttgaaccaca ggaacgcctc cggcgcccgg cccttgatcg ggccgactcc gctcgacccc
     1681 gtcacgaacc cggtgttgct gttcacgccc gccatcgtgt tccagaacag gaccccgaac
     1741 atcgtgccga gggtcaggaa gaggaccgtg aagaaggtca cgaccgtggc gccctgggtc
     1801 cgttgcatga tcgccgagat gaagaggccc atcgatccga ggccgagcgc ggtgacgatc
     1861 aggacaacgt aggcccgcag gacgtcgtcg ggggcgacgc cgccgaagac gaagacgatg
     1921 gccgtcaacg ggatcgaggc ggcgatgagg aggaacacgt aggcgagggc gctcaggagc
     1981 ttgccgacga cgatcgccag cgacgagatc ggcgtggcgg tcagcatgtc gagggtctgc
     2041 ttctcgcgtt cgaggctgat cgcgcccgcg gtgaacgccg gggcgaggaa gacgacgagc
     2101 agcgtctcga cgagcaccag ggcgccgaag acctcctggc cgacgagcga cgaggcgagg
     2161 gtggccgacc cgccctgcag ggcggcattc tggacgtaga ccacttcctc gcggagctcc
     2221 cacgcccacg cgaacagcgc caggaacagc aggtagaacg tcaggacgac gaaggcccgc
     2281 cggccgcgga cccggccgcg gagctccttg accccgactg cgatcacgcc gtcgacgcca
     2341 gaccgaaagg ctcggaccgg gttgggacgg ggacgtcgcg gtcgcgccga atgagcgccg
     2401 accgactcgg tccggacggc gcccggcgca gcgctgtcga ccgtcgtctc ctcgatcatc
     2461 ggcccgggct ggggcgcttc ctcggcgggc ggagtgactc gttcgtcggt cacgtcaggc
     2521 cacctgcgtc tgctggccgg ccgtcgcggt gacctggagg aagagctcct cgagatcgct
     2581 cgcggcgcgg gcgaacgacg tgatcctgat tccggccgcc acggccgccg ccaggagctg
     2641 tgcagaggcc tcgtcgtcgc cgcggaaccc gagctcgatc tggccgtcgt cgaggatggt
     2701 cgccgaggcg acgcgcggat cggcggcgaa gtggttgcgc gcatcctcgc ggctgtggcc
     2761 ggtcaggatc cggacccgga gcacggcccc gaaccggagg cgccgctcga tctcgctgac
     2821 cttgccgtgg gcgagcacct ggccatggtc gacgatcgcg accgcggtgc agagctcctc
     2881 gagctcggga aggatgtggc tgctgatcac gatcgtcttg ccgagcgcgc gcagctcccg
     2941 gaggagctca cggagctcga cccgggcccg tgggtccagg ccggaagcgg gttcgtcgag
     3001 gaggagcacc tcggggtcgt ggacgagggt gtgggcgagg cagagtcgct gctgcattcc
     3061 gcgcgacagg ccctggacgt aggcgtcgcg cttttcgccg agatcgacga ggtccaggag
     3121 gtcgccgatc atccgccgtc gccgggatgc cgggatgccg tagcaccggg cgaagaagtc
     3181 gaggtactcc cagaccttca tgtcgtcgta gaccccgaac acatccggca tgaagccgag
     3241 gacgcgacgg gccgcatcgg ggttccgccg gacgtcgatg ccggcgatct cggcgtgccc
     3301 ggacgtcgcg ctgagcagcg tggccaggat ccgcagcgtc gtggtcttgc ctgcgccatt
     3361 cggcccgacc aggccgaaga tctcgccctc accgacggcg aggtcgagcc cggccacggc
     3421 caacgtcccg ggatagcgct tgacgaggcc gcgggtgacg acgatgtcgg tcacttgacc
     3481 gtcccttcga tcgacaggtt gagatagacg ttgaccggat cctggcgctc gttgacgaag
     3541 cggacgagga cggcaccagt gctcggatcg acgtacttcg ccgcgtccgc cacatcgtag
     3601 gtctgacctt gcgagaggtg cggcaggcgg tgccaggtcc cgtcgccggt ccgatcgaac
     3661 agctcgacct ccggcatgcc atcgaactgg tcggcgggca gcgccttcgg gcagttggcc
     3721 ggcggcttgg cgggcgcctt gacgttcagg caggcgtccg ggatcggggc gaccttgacg
     3781 ccactgacgc cgttgaccga gccgtccggc ccggagctca cgccgagccg cacgtgcgtc
     3841 gcggtcagcg tgccgtcgaa ggcgatcggc cggtagacca tcgtcacgct gccctggccg
     3901 aagctcagcg tcatcggatc cttgttgaag aacgcggcat cggtctcggt catcgtgctc
     3961 ttcacgagat ctccgctgaa cgcaacggac ccgtggacgc ccatcgccag cggcacgtaa
     4021 tagaggacgt tgctcacccg gttcgtcgcc tggccctcga ccgcgacgtc catgaccgcg
     4081 tcgcggcccc aggcaagaag gacgggaccg tcggacgaca gccggccgaa gttgccgaac
     4141 tgcgggtcat acgtcagctg gtcgaccacg agatggcggg tctggtcgcg gcgggcggtt
     4201 tcggtcgtcg cgcccgtctg gccgaagaag atctggccga agatccggtc cgagagcgac
     4261 tggccgaacg cgttctggtt cgtgatcgcg agcgagacct ggacctgctc cccgggctgg
     4321 agatccttga ggaccttgac gttgctgccg aggacgatcg ccggcttctc gagcttgacc
     4381 gccgaatcgt tgcggatcgt accggtcagc gtcccgtcga cgagggccag ctcggcgtgg
     4441 accttcggca ccacggcctg cgactcggcc cggatcgtcc gcagcgagcc gaacccgacc
     4501 gacaggtcgc ggacgcgcga gggtccgggc gggtcgccct ggacgatgtc gaggctggcc
     4561 gtcgtcccgc cgaacacgtc gcccgagatc ggcgaggaca ggagggcgcc gcccgggacc
     4621 gacacctggt aggtgccgcg ggtcggcgag aagaccccca ggtacacctg ggcgctgccc
     4681 tcggtcgcgt ccggagcgcc gcggacgatg ccgacctcgt tgacgatgat gctgctgccc
     4741 cggagcgccg acccaaaggc gtaggcaccg gctgcgaagc cgacgatcag gatcggcatc
     4801 gtgatccatg cccactcgcg ccggtcgagg cgccacagga tcaagtagtt gagcgggccg
     4861 atcagcgcga tgtagccgaa caggagcagg agcaggccgc cgagcggcgg gagggcgagc
     4921 gacggcaggt tgctgacggc gctgacgatc tggctgtcgt cggtcatgcc gacactccca
     4981 tcggagcggg ccgggatcag cgatggccag agggatcggc tggcggtggc ctgggcgacc
     5041 cagccgacgg tcgggtcgat cccgaggatc gtcacgctgc cgctgccgta cggcgtctgc
     5101 gcagccacga cccggtcgcc gctcgtcgcc agggcgcggc cccggatcag gtcgccggcc
     5161 atcgccggga catcgccggc gtccttcggt ggcgtcccca gcagcgagat cagcgacgcg
     5221 gcggccacat cgacggtcga ggtcggtcga tagggcagga tgtcgtcggg gaaaccgccc
     5281 agcgagccga tcccgccggt tccgccgacg atgaccaacc ggccgccgag ggccagccag
     5341 ccgcgcaggg cggtcagctg ctccttgctg agggttgacg catcgacgtc ctgccagatg
     5401 aggcggtcga gcgccgacca ggcctcgatc cgggtcggca ggtcggccac ggtcaggggc
     5461 acgacgaccg ccggctgctg gttctggacg gcggggaggc tcaggctgcc gacgatcttc
     5521 ggggcggcct ccgcgacgac tccgacgaca agctggccgg ggtcgtggat cgtgaccgcc
     5581 accttctggc gaagcaggat ctgaccgccg ccgaccagga cgacctcgag ctgctggccg
     5641 aaggacggcg gctgggcgtg gagcacccag gtcttgtcgg acgggctgtc gagctgaacg
     5701 gccgtcgcgt accgcgtccc gccctggctg ccgccctgga gccggatctc gccgctgatc
     5761 gacgggcccg agttgacgag gtggacctcg atcgcgatcc acgagccgag acgggcgtga
     5821 ccctggagga ggacccgggc ggtcatcgtc ggtggatcgg ccgcgacagc accggccgcg
     5881 gccggaccga gggcggcgac gacgagcagc gaggcggtcg ccagcagaac gacgaggcgg
     5941 aggatccggc gtggcgcgtg acgggtcacg gggactccag gtaggggaac gacttgcggt
     6001 cgcagcatcg acgtcccggc cccccgaaaa gttgcgctcc cggtcgcggg accggccaga
     6061 cggatgtccg ctcggccaga cccgaacggc gacggcacgt cgggcgcggc tatcgtacga
     6121 tgccgggagg gggcggccgt tgcggcgtca ctcgctcgtg gccagccgcc gaccgcccgt
     6181 cgcacgaccg tcgacccgag gaacccgtgc cccagatccg tccctttcgc gcccttcgct
     6241 atgcccccga cagcgtcggc gacgtcgccg ccgtggtcgc tccaccctac gacgtcctga
     6301 ccgctgcgga ccgcgctcgc ctcctggccc gccatccgaa gaacgtcgtc cggctcgatg
     6361 cgccgaccga cgaggccggt gacgcggagg acgatcgcta tcgacgcgcc gcccggacgc
     6421 tcgcggcctg gcgttcggac ggaacgctgc acaaggatcc tcgacccgcg atctacgtcc
     6481 acgagcagac ctacctggtc cccggcacgg acgtcgagcg gacccagcgc ggcttcttcg
     6541 cgcgcctgcg cctggaggcg ctggttccgc cggccggcgt cctgccccac gagcggacgc
     6601 tcgcaggccc gcgcgaggac cgttaccggc tgctccgggc gaccggcgtg aacacgagcc
     6661 cggtgatcgg cctgtacgac gatccgggcg ggcacgccgc ggcgatcctg gcgacgctcg
     6721 cgaccgggac ggccgcgatc gacgtcgtcg acgacgacgg cgtacgtcac cgggtgtgga
     6781 tcgtcgatgc cgacggaccg gcttcgaacg ccgtgtccga gctggtggcg atcgcggccg
     6841 cggacccggt gacgatcgcc gatggccacc atcgctacga gacagccctg cgctatcgcg
     6901 acgagcgccg gatgagccgc tcgtgcgagg aggacccggc attcgactac ctgctcatgc
     6961 tgttcctcga gacgaatgct gagccgttga cgatcctgtc gacccatcgc ctcgtcgcga
     7021 tagcgcaggg ctgtctcgta gcgatggtgg ccatcggcga tcgtcaccgg gtccgcggcc
     7081 gcgatcgcca ccagctcgga cacggcgttc gaagc
//
LOCUS       DATAIW010000294         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_246408, whole genome shotgun sequence.
ACCESSION   DATAIW010000294 DATAIW010000000
VERSION     DATAIW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246408"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..840)
                     /locus_tag="VHM48_09175"
     CDS             complement(10..840)
                     /locus_tag="VHM48_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755623.1"
                     /translation="MTFERNPGPPPEPGPTPVVGSAAPGSVPTPVQVRLQILSTEHWS
                     LLASRSLAWNEIFSRAGMFLSTLSGSMVALGLIGGIDGFGDPFFTFALVILPVVLFVG
                     LGTWARMGVANYHDAMTVYGMNRIRGAYLEIAPEMEPYFVMGVHDDPQGIGVTMGMPP
                     GTSMVVHLISSTPFLIVVLNAVLAGAIAALAFIRFVNASGQVTLAGAIIVALVLLGIQ
                     LGYARGRIRAGQNAVVPMFPTPDAGGARETIFGAELPRPSGRTMPGADRRDPPDRPGA
                     "
     gene            complement(898..1965)
                     /locus_tag="VHM48_09180"
     CDS             complement(898..1965)
                     /locus_tag="VHM48_09180"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX2755624.1"
                     /translation="MTDRPAPIDLRSDTVTRPTPGMREAMARADVGDDQFGEDPSINR
                     LQERVAAMLGHEAALWFPTGTMANQVAIRTLTRPGDEVLAPREAHILWHETGAAAANS
                     GVQVTEIGPAVTTGRFTADEFLAARKPRGHMLLPPTTVVDVENTHNRAGGVVIDVATS
                     TAIADAARANDVTSFLDGARLWNAAVALGVDIAELARPFDVVNVAFSKGLGAPGGSLL
                     AGSRAFVAAATRYRRMAGGAMRQVGIFAAAAEFALDHHIDRLAEDHANAHLLADRLAA
                     SPAIELDPAAVQTNIVVFRLADGAPDAPTVVAAAREAGVLVVAFSAGTIRAVTHLDVT
                     RAEVADAAELLVAAAESDAPG"
     gene            2010..4025
                     /locus_tag="VHM48_09185"
     CDS             2010..4025
                     /locus_tag="VHM48_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2755625.1"
                     /translation="MPAARPIFGRDVPDAAWRPSRDLLADARLAQFLRATGEPDVEAL
                     QARAVADPAWFWGAAVDDLGLTWQRPPDQVMDASGGPEWTRWWRGGAFNHAEAATAPR
                     AERDPDGGAIAWEGEDGEVRRLTNRQLRDTVVAAARMFVAQGVRPGDRVGIFLPMLVE
                     TVVATLALGRIGAIFSPIFSGYGAPAVAARLQDCEASVLVTADGFLRRGSVVPMKAVA
                     DEAAALAPTVRRTIVVRRLGDRAPATPWNPERDRWWDEALDGARAAIIDEGGPEPAAW
                     TDPETPYMLIYTSGTTGRPKGAVHVHGGFPIKAAQDLAHTFDLRAGDTLFWFTDLGWM
                     MGPWAISGSLLLGARLVLYEGAPDFPGPDRLWALVARHGVTHLGLSPTVVRALIPHGT
                     GPILAHDLAALRVLGSTGEPWDPDSWWWYFREVGGGRCPVVNYSGGTEVSGGIVGCNL
                     LTPIKPTSFNGPCVGTAADVVDPAGRSLRGEVGELVIRAPQPGMTRGFWLDPDRYVEA
                     YWSRIPGTWVHGDWAIVEDDGYWYIRGRSDDTLKVAGKRVGPAEVEAAATAHPSVVEA
                     AAIGVPHAIKGETIVVVCTLRRGETDDEALRAAIARRVVEDLGKTLKPEAIAVVPALP
                     KTRSGKIMRRVVRAAWLGLDPGDLSALDDPTTIDAIRRAGPTGGAIG"
     gene            4061..4525
                     /locus_tag="VHM48_09190"
     CDS             4061..4525
                     /locus_tag="VHM48_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2755626.1"
                     /translation="MSVAIEKDIVTWDALANMVAELASQVRGEYDVMLAITRGGLVPA
                     GMLAYRLGIRNILVAAVEYYDDEGKPGPHPTFLQFPADPLLRGQRVLVVDEVWDSGTT
                     IHAVTERVRQAGGEPTTAVLHYKPARSHVPGSPDHHVVTTDRWVVYPFKAGN"
     gene            4612..5688
                     /locus_tag="VHM48_09195"
     CDS             4612..5688
                     /locus_tag="VHM48_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755627.1"
                     /translation="MTRTLLLVGTRKGAFILDGGTERADWSVSDPMCEGWPIHDLQWD
                     PTTGSIYAGGGSPWFGAAVFRSDDLGATWTHSSEGLTYGDDGPKIPAVWNVTPANGSI
                     YAGVEPAGLFRSDDGGATWSHVSALRDHPSRQSPGWQPGAGGLICHTIVPHPTDPQRM
                     WIAISAVGTFATEDGGASWEARNKGVAACFMPDPHPETGQCVHKLVMAAGEPDSLYQQ
                     NHCGVYRSRDAGRSWDDISAGLSSEFGFVMGTHPRDPATAWVVPLSHPEEGRYAPDGA
                     LAVWRTRDRGETWERHGDGLPQEHAYVGVLREAFSVDRGDPAGTYFGTSTGQLYGSLD
                     EGVTWRLIADHLPSIWGVQAVEVD"
     gene            5693..5959
                     /locus_tag="VHM48_09200"
     CDS             5693..5959
                     /locus_tag="VHM48_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2755628.1"
                     /translation="MAVIVILPRSLVSLIPGAARSTEVDATTVREAIDRLDERTPGVR
                     NRLVDSGPTLREHINVFVDGTQASLDTPLRTGSTIHVIPAVSGG"
BASE COUNT          857 a         2150 c         2244 g          838 t
ORIGIN      
        1 gctcggcggt cacgctccgg gccgatcggg cggatcgcgg cggtccgctc ccggcatggt
       61 ccggccgctc ggacggggta gttcggcccc gaagatcgtc tcccgggcgc caccggcatc
      121 gggggtcggg aacatcggga cgaccgcgtt ctggcccgcg cggatccggc cccgcgcgta
      181 gcccagctgg atgccgagca ggaccagcgc gacgatgatc gcgccggcga gcgtcacctg
      241 gccgctggcg ttgacgaacc ggatgaacgc gagggcggcg atggccccgg cgaggacggc
      301 attcagcacc acgatcagaa acggcgtcga cgagatgaga tggacgacca tcgacgtccc
      361 gggcggcatc cccatcgtga ccccgatgcc ctggggatcg tcgtggaccc ccatcacgaa
      421 atagggctcc atctccgggg cgatctcgag ataggcaccg cggatccggt tcatgccgta
      481 gaccgtcatc gcgtcgtggt agttggccac gcccatccgg gcccacgtgc ccagcccgac
      541 gaacaacacg accggcagga tcacaagggc gaacgtgaag aagggatcgc cgaagccgtc
      601 gatcccgccg atcaggccga gcgcgaccat cgaacccgac agcgttgaga ggaacatgcc
      661 ggctcggctg aagatctcgt tccaggcgag gctccgcgac gcaagcagcg accagtgctc
      721 ggtcgagagg atctggagcc ggacctgaac cggggtcggc actgagccgg gcgcggccga
      781 gccgacgacc ggggtcggcc cgggctcggg cggtggaccg ggattgcgct cgaacgtcat
      841 cgctggctcc ctcagtgacg ccctcgtcga tcgatgcgca gtatcccgcg cggcgcgtca
      901 gccgggggcg tcggattcgg cggccgcgac gagaagctcg gccgcgtccg cgacctcggc
      961 ccgcgtcacg tcgaggtggg tgaccgcgcg gatcgtcccg gcgctgaacg cgacgacgag
     1021 cacgccggcc tcacgggcgg ccgcgacgac cgtcggcgcg tctggtgcgc cgtcggccag
     1081 gcggaagacg acgatgttcg tctgtacggc ggccggatca agctcgatcg ccgggctggc
     1141 ggcgagccgg tccgcgagga ggtgggcgtt ggcgtggtcc tcggcgagtc ggtcgatgtg
     1201 gtggtcgagc gcgaattccg ccgcggccgc gaagatcccg acctggcgca tcgctccgcc
     1261 ggccatccgg cggtagcggg tcgcggccgc cacgaacgct cgcgacccgg ccagcaacga
     1321 gccacccggc gcaccgagcc ctttcgagaa cgcgacgttc acgacgtcga acggccgggc
     1381 cagctcggcg atgtcgacgc cgagggccac ggcggcattc cacagccggg ccccgtcgag
     1441 aaagctggtg acatcgttgg cccgggcggc gtcggcgatc gcggtgctcg tcgccacatc
     1501 gatcaccacg ccgccggccc ggttgtgggt gttctcgaca tccacgaccg tcgtcggcgg
     1561 caggagcatg tggccgcgcg gcttgcgggc ggccaggaac tcgtccgcgg tgaagcgccc
     1621 ggtcgtgacg gccggcccga tctccgtgac ctggacgccg ctgttggccg cggcggcgcc
     1681 ggtctcgtgc cagaggatgt gggcttcgcg gggggcgagc acctcgtcgc cgggccgggt
     1741 gagggtccgg atcgcgacct ggttggccat cgtcccggtc gggaaccaga gcgccgcttc
     1801 gtgaccgagc atggccgcaa cccgctcctg gagccggttg atcgaagggt cctcgccgaa
     1861 ctggtcgtcg ccgacgtcgg ccctggccat cgcctcgcgc atgcccgggg tcgggcgggt
     1921 gacggtgtcg gagcggaggt cgatcggcgc gggtcggtct gtcatcggcg aagcgtagcg
     1981 ccgccgccgg gtggcgtagg ctcttctcga tgcccgccgc ccgcccgatc ttcggccgcg
     2041 atgtgcccga cgccgcctgg cggccgagcc gggatctcct ggcggacgcg cgcctcgccc
     2101 aattcctccg ggcgaccggc gagcccgacg tcgaggcgct ccaggcccgc gccgtcgccg
     2161 acccggcatg gttctggggt gccgccgtcg acgatctcgg gctcacctgg cagcggccac
     2221 cggaccaggt catggacgcg agcggcggcc ccgagtggac gcgctggtgg cgcggtggag
     2281 cgttcaacca cgcggaggcg gccacggcgc cgcgcgccga acgggatccg gacggcggcg
     2341 cgatcgcgtg ggagggcgag gacggcgagg tccgccggct cacgaaccgc cagctccggg
     2401 acacggtcgt cgccgcagcc cggatgttcg tcgcccaggg cgtccgaccc ggcgaccggg
     2461 tcgggatctt cctgccgatg ctcgtcgaaa cggtcgtggc aacgctggcc ctggggcgca
     2521 tcggcgcgat cttcagcccg atcttctcgg gctacggggc gccggcggtg gcggcccggc
     2581 tccaggactg cgaggcgagc gtcctcgtca cggccgacgg cttcctccgc cgcggatcgg
     2641 tcgtgccgat gaaggcggtc gccgacgaag cggccgcgct cgcaccgacg gttcgccgca
     2701 cgatcgtcgt tcggcggctc ggcgatcggg cgccggcgac gccatggaac cccgagcgcg
     2761 accgatggtg ggacgaggcg ctcgacggag cgcgggccgc gatcatcgac gaaggcggcc
     2821 cggagcccgc cgcctggacc gaccccgaga cgccctacat gctgatctac acctcgggca
     2881 ccacgggccg gccgaagggc gccgtccacg tccacggcgg cttcccgatc aaggccgccc
     2941 aggacctcgc ccacaccttc gacctccgcg ccggcgacac cctgttctgg ttcaccgacc
     3001 tcggttggat gatgggtccg tgggcgatct cgggctcgct cctgctcggc gcccggctcg
     3061 tgctgtacga gggcgcgccg gacttccccg gtccggaccg gctgtgggcg ctggtcgccc
     3121 gtcacggcgt gacccatctc ggcctgtcgc cgacggtcgt gcgggccctg atcccgcacg
     3181 ggacgggtcc gatcttggcc cacgacctcg ccgcgctccg ggtcctgggc tcgaccggcg
     3241 agccgtggga tcccgacagc tggtggtggt acttccgcga ggtcggaggc ggtcgctgtc
     3301 cggtcgtcaa ctactcgggc gggacggagg tcagcggcgg catcgtcggc tgcaacctgc
     3361 tgacgccgat caagcccacg tcgttcaacg gaccgtgtgt cggcacggcg gcggacgtcg
     3421 tcgaccctgc gggccggtcg ctccgcggcg aagtcggcga gctcgtcatc cgggcaccgc
     3481 agccggggat gacccgcggc ttctggctcg accccgatcg ctacgtcgag gcgtactgga
     3541 gccggatccc cggcacgtgg gtccacggcg actgggcgat cgtcgaggac gatggctact
     3601 ggtacatccg cggccgctcg gacgacaccc tcaaggtcgc cggcaagcgg gtcggcccgg
     3661 ccgaggtcga agccgcggcg acggcccacc cgagcgtggt cgaggcggcg gcgatcggcg
     3721 tcccgcacgc gatcaagggc gagacgatcg tcgtcgtctg cacgcttcgg cgcggtgaga
     3781 ccgacgacga ggcattgcgc gccgcgatcg cccggcgtgt cgtcgaggac ctcggcaaga
     3841 ccctcaagcc ggaggcgatc gccgtcgtgc ccgcactgcc gaagacgcga tcgggcaaga
     3901 tcatgcgacg cgtcgtccgg gcggcgtggc tcggcctcga tcccggggac ctgtcggcgc
     3961 tcgacgatcc gacgacgatc gacgcgatcc gccgggcagg cccgaccggc ggggcgatcg
     4021 gctgacggcc gggatggcga agcgaggagc ggatcgacag atgagcgtcg cgatcgagaa
     4081 ggacatcgtc acctgggacg ccctcgccaa catggtcgcg gagctcgcga gccaggtgcg
     4141 cggcgagtac gacgtcatgc tcgcgatcac ccgcggcggg ctcgttccgg ccgggatgct
     4201 cgcctaccgg ctgggaatcc ggaacatcct cgtcgcggcc gtcgagtatt acgacgacga
     4261 gggcaagccc ggtccccacc cgacgttcct ccagttcccg gccgatcccc tcctccgcgg
     4321 ccagcgggtc ctggtcgtcg acgaggtctg ggacagcggg acgacgatcc acgcggtcac
     4381 cgagcgggtt cgccaggccg gtggcgagcc gaccaccgcg gtgcttcact acaagccggc
     4441 tcgctcccat gttccgggct ctcctgacca tcacgtcgtg acgaccgatc gctgggtcgt
     4501 ctatccgttc aaggcgggca actgagaccc ggccgccgtt cgaccgatga gaacggagct
     4561 cgagggtcgt ctgtatcggc gaccaggcca cataccgctg gagggatccc gatgacccgc
     4621 acgctgctcc tcgtcggcac tcgcaagggc gccttcatcc tcgacggcgg cacggagcgg
     4681 gcggactggt ccgtgtccga cccgatgtgc gagggctggc cgattcatga cctccagtgg
     4741 gatccgacga ccggttcgat ctacgccggt ggcggctcgc cgtggttcgg cgcggcggtg
     4801 ttccggagcg acgatctcgg ggccacctgg acgcattcgt cggagggcct gacgtacggc
     4861 gacgacggcc cgaagatccc ggcggtctgg aacgtgaccc cggccaacgg ctcgatctat
     4921 gccggcgtcg agccggccgg cctgttccgg agcgatgacg gcggggcgac gtggagccac
     4981 gtctcggcgc tccgcgacca ccccagccgc cagtcgccgg gctggcagcc gggcgccggc
     5041 gggctgatct gtcacacgat cgtcccccat ccgaccgacc cgcagcggat gtggatcgcg
     5101 atcagcgcgg tcgggacgtt cgcgaccgag gacggcggcg cctcgtggga ggcgcgcaac
     5161 aagggcgtcg cggcgtgctt catgcccgat ccccaccccg agaccggcca gtgcgtccac
     5221 aagctcgtga tggcggcggg cgagcccgac agcctctacc agcagaacca ctgcggcgtc
     5281 tatcgatcgc gcgacgccgg gcggagctgg gacgacatca gcgccggcct gtcgtccgag
     5341 ttcgggttcg tgatgggcac ccacccgcgc gacccggcga ccgcctgggt cgtgcccctc
     5401 agccaccccg aggaaggacg gtatgcaccc gatggcgccc tggccgtgtg gcggacgcgc
     5461 gaccgcggcg agacatggga gcgccacggc gacgggctgc cgcaggagca tgcctatgtc
     5521 ggcgtgctgc gcgaggcgtt ctcggtcgat cgcggcgatc cggccgggac ctatttcgga
     5581 acgagcacgg gccagctcta cggctcgctc gacgagggcg tcacgtggcg gctgatcgcg
     5641 gatcacctgc cgtcgatctg gggcgtccag gccgtcgagg tcgattgacg tcgtggcggt
     5701 gatcgtcatc ctgccgcgct cgctcgtgtc gctgatcccg ggcgcggccc gatcgaccga
     5761 ggtcgatgcg acgaccgtcc gcgaggcgat cgaccgcctc gacgaacgga cgccgggcgt
     5821 gcgcaaccgt ctcgtcgaca gcggaccgac gctgcgcgag cacatcaacg tgttcgtcga
     5881 tgggacgcag gcctcgctcg acacgccgct gcggaccggc tcgacgatcc acgtgatccc
     5941 ggccgtgtcg ggcggctgag gggcgcggcc gggcgttgtc gcgtggccgg aacgagacgg
     6001 ccagcggccc gtgcgccagc ggcccgtgac gacgccgatc ttgccgcacg gccgccaatg
     6061 agagcggccg caggcgctgc gtatagccg
//
LOCUS       DATAIW010000295         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_276309, whole genome shotgun sequence.
ACCESSION   DATAIW010000295 DATAIW010000000
VERSION     DATAIW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276309"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VHM48_09205"
     CDS             <1..1330
                     /locus_tag="VHM48_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2755629.1"
                     /translation="VTVNPATEEPLAVVARATAADMDRAVRAARSAFRRSWSTLSGRE
                     RAKYMFRIARILQERSREFAVLESMDTGKPIKESRDVDIPLAAAHFWYYAGWADKLEY
                     AFPGRVAKPLGVAAQIIPWNFPLLMLAWKIAPALAAGNTVVLKPASTTPLSALLFADV
                     CRQADLPPGVVNIVPGPGAIGMSLVTHPGVDKVAFTGSTEIGRQIFKGVAGTDKGLTL
                     ELGGKAANIVFDDAPLDQAVEGIVNGIYFNQGEVCCAGSRLLAQESIVQPLIEKLKDR
                     LATLRVGDPLDKNTDVGAINSKAQLEKITELVAAGIAEGAEMYQPACDLPERGFFFRP
                     TLFTNVAQSHRIAREEIFGPVLSVLTFRTPEEAVEKANNTPYGLSAGIWTDKGSRILH
                     MVKRMKAGVVWANTFNRFDPTSPFGGYKESGFGREGGMHGLHAYVRLEDR"
     gene            1332..2222
                     /locus_tag="VHM48_09210"
     CDS             1332..2222
                     /locus_tag="VHM48_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2755630.1"
                     /translation="MAAARIDVRKTYKLYIGGAFPRSESGRSYAVTAANGDPLANACR
                     ASRKDLREAVREARKAFQPWADKTAMNRGQVLYRVAELMEGRRDQFVAEVAAAEGLRE
                     VKAGEVVDRAIDRWVWYAGWADKIAQVLGSSNPVAAPYFNFTIPEPTGVVGIVAPETS
                     SLLGLVSRLAPPLVTGNAVVVLTSETRPLPAVTLAEVLATSDVPGGVVNILTGLKKEL
                     VPVLAGHVDVDTIDVWGVPADLRTETELIAADNIKRIARRPAGVTDARFDWLDDRATE
                     RPEWIAAYLEMKTVWHPIGV"
     gene            2512..2991
                     /locus_tag="VHM48_09215"
     CDS             2512..2991
                     /locus_tag="VHM48_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2755631.1"
                     /translation="MTPGESQFVEEMGQFLGSLGMTPMSGRMWGWLLICEPVEKTAAE
                     IAAALHASRGAISGTARLLATAGLIRRSTRAGDRREYFSAPAEALDSMLASAGAIYRQ
                     MREIAERGLAATADRSPAARDRLREFHDVMTFVEQEVPRLIGRFLADRAAEKQRLAG"
     gene            3048..>3767
                     /locus_tag="VHM48_09220"
     CDS             3048..>3767
                     /locus_tag="VHM48_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755632.1"
                     /translation="MTAIIQTEKLTKFYGTHRGIVDVDLEVGNGEVFGFLGPNGAGKT
                     TTIRTLLDLIRPTSGRAFVFGIETSVDPVAIHRRIGYIPGEFALYDRLTGGQTIEYFA
                     NLRGGVDRAYQRELVDRFDVDPSRRFKEYSKGNKQKVGLVIALQHRPELLVLDEPTSG
                     LDPLVQQSFYQLVRDAQAEGRTVFLSSHILSEVERTCDRVAIIRDGLLVKVDSVEGLR
                     DLAHHEVELRFVDGVPAEAFSG"
BASE COUNT          584 a         1357 c         1251 g          575 t
ORIGIN      
        1 cgtgacggtc aatccggcga ccgaggagcc cctcgccgtg gtcgcccggg ccaccgcggc
       61 cgacatggac agggcggtcc gcgcggcacg cagcgccttc cgccggtcct ggagcacgct
      121 gtccggcagg gagcgcgcca aatacatgtt ccggatcgcc cggatcctcc aggagcgctc
      181 gcgcgagttc gccgtcctcg agtcgatgga cactggcaag ccgatcaagg agtcgcgcga
      241 cgtcgacatt ccgcttgcgg cggcccactt ctggtactac gccggctggg ccgacaagct
      301 cgaatacgcg ttcccgggtc gcgtcgcgaa gccgctcggc gtggccgccc agatcatccc
      361 gtggaacttc ccgctcctga tgctcgcctg gaagatcgca ccggcccttg cggccggcaa
      421 cacggtcgtc ctcaagcccg cctcgacgac gccgctgtcc gcgcttctct tcgccgacgt
      481 ctgccgccag gccgacctgc cgccgggcgt cgtcaacatc gtcccgggtc cgggcgcgat
      541 cgggatgtcg ctcgtcactc atcccggcgt cgacaaggtc gccttcaccg gctcgaccga
      601 gatcggccgc cagatcttca agggcgtcgc cggcaccgac aagggcctga ccctcgagct
      661 cggcggcaag gccgccaaca tcgtcttcga cgacgcgccc ctcgaccagg ccgtcgaggg
      721 catcgtcaac gggatctact tcaaccaggg cgaagtctgc tgcgcgggat cgcggctgct
      781 tgcccaggag tcgatcgtgc agccgctgat cgagaagctc aaggaccgac tcgcgacgct
      841 gcgggtcggc gatccgctcg acaagaacac cgacgtcggg gcgatcaact cgaaggccca
      901 gctcgagaag atcaccgagc tcgtggcagc ggggatcgcc gagggtgccg agatgtacca
      961 gccggcctgc gacctcccgg agcgcggctt cttcttccgc ccgaccttgt tcacgaacgt
     1021 cgcccaatcg caccgcatcg cgcgcgagga gatcttcggg ccggtcctgt cggtgctcac
     1081 cttccggacg ccggaggagg cggtcgagaa ggcgaacaac acaccgtacg gcctgtcggc
     1141 cgggatctgg accgacaagg gcagccggat cctccacatg gtcaagcgga tgaaggcggg
     1201 cgtcgtctgg gccaacacgt tcaaccggtt cgacccgacc tcgccgttcg gcggctacaa
     1261 ggagtcgggc ttcggccgcg agggcgggat gcacggcctt cacgcctacg tccggctcga
     1321 ggaccgctga cgtggccgcc gcccggatcg acgtccgcaa gacctacaag ctctacatcg
     1381 gcggggcgtt cccgcggtcg gagagcggcc gctcgtacgc cgtgaccgcc gccaacgggg
     1441 atcccctcgc caacgcctgc cgcgcctcgc gcaaggacct ccgcgaggcc gtccgcgagg
     1501 ctcgcaaggc gttccagccg tgggccgaca agaccgcgat gaaccgcggc caggtcctct
     1561 accgggtcgc cgagctgatg gagggccggc gcgaccagtt cgtcgccgag gtggccgccg
     1621 cggaagggct gcgtgaggtg aaggccggcg aggtcgtcga ccgggcgatc gaccgctggg
     1681 tctggtatgc gggctgggcc gacaagatcg cccaggtcct cggctcgtcg aacccggtcg
     1741 ccgcgccgta cttcaacttc acgatccccg agcccacggg cgtcgtcggg atcgtcgccc
     1801 ccgagacgtc gtcgctgctc ggcctcgtgt cgcgcctcgc gccaccgctc gtgaccggca
     1861 acgcggtcgt cgtcctgacc tccgagaccc ggccgctgcc ggcggtcacg ctcgcggagg
     1921 tcctcgccac ctcggacgtc ccgggcggcg tcgtgaacat cctcaccggc ctgaagaagg
     1981 agctggtccc ggtcctcgcc ggccacgtcg acgtcgacac gatcgacgtc tggggcgtgc
     2041 cggccgacct ccggaccgag acggagctga tcgccgcgga caacatcaag cggatcgccc
     2101 ggcgcccggc cggcgtgacc gatgcccgct tcgactggct cgacgaccgg gccacggagc
     2161 gcccggagtg gatcgcggcc tatctcgaga tgaagaccgt ctggcacccg atcggggtct
     2221 gatcgcttcg cagggcatgt cgatggcccg tgcgatctcc gaatgcgcgc cgggtgagca
     2281 ctacggcggc ggaagcccga tcatcgggcc ctggaaggcg tcgtcgaccc gtttgttagt
     2341 cgtcgagggc taactcggct cgaacgacgc ccgtttggct gcgtttcttc gtcgtaacgc
     2401 ccgctgggcg cgcatctcgc gcatgggcgc gccacggcgc gtcgcgaccg cactccacgt
     2461 caccaacgtt cagtattgac tgaaacttcc gaacgttcta tcgtccggtg catgacgccc
     2521 ggcgagagcc agttcgtgga ggagatgggc cagttcctcg gttccctcgg catgacgccg
     2581 atgtcgggcc ggatgtgggg ctggctgttg atctgcgagc cggtcgagaa gaccgctgcc
     2641 gagatcgccg ccgccctcca cgccagccgc ggcgcgatca gcggaacggc ccggctcctc
     2701 gcgacggccg ggctgatccg ccgctcgacc cgggccggcg accggcgcga gtacttcagc
     2761 gctccggcgg aggccctcga ctcgatgctc gcgagcgccg gggcgatcta ccgccagatg
     2821 cgggagatcg cggagcgggg cctcgccgcg accgccgacc gctcgccggc ggcccgtgac
     2881 cggctccggg agtttcacga cgtcatgacg ttcgtcgagc aggaggttcc gcgcctgatc
     2941 ggccggttcc tcgccgatcg agcggccgag aagcaacgtc tcgcgggcta gccccatcgg
     3001 catcccgccc gacctggccg gcccactgcc ggcagaaggg accatcgatg accgcgatca
     3061 tccagacaga gaagctcacc aagttctacg gcacccatcg cggcatcgtc gatgtcgacc
     3121 tcgaggtcgg aaacggcgaa gtgttcggct tcctcggccc caacggcgcc ggcaagacga
     3181 caacgatcag gaccctcctc gacctcatcc gtcccacgtc gggtcgtgcg ttcgtgttcg
     3241 ggatcgagac gagcgtcgac ccggtcgcca tccaccgccg gatcggctac atcccgggcg
     3301 agttcgcgct ctacgatcgg ctcaccggcg ggcagacgat cgagtatttc gcaaacctcc
     3361 gcggtggcgt cgatcgcgca tatcagcgcg agctggtcga ccggttcgac gtcgacccct
     3421 cgcgccggtt caaggagtac tcgaagggca acaagcagaa agtcgggctc gtcatcgccc
     3481 tccagcatcg tccggagctc ctcgtcctcg acgagccgac gtcgggcctc gacccgctcg
     3541 tccagcagtc cttctaccag ctcgtccgag atgcccaggc cgagggccgg accgtcttcc
     3601 tgtccagcca catcctgtcc gaggttgagc ggacctgcga tcgcgtcgcg atcatccgtg
     3661 acggactcct ggtgaaggtc gacagcgtcg aaggtctgcg cgacctcgcc caccacgagg
     3721 tggagctgcg cttcgttgac ggcgtcccgg ccgaggcctt cagcggc
//
LOCUS       DATAIW010000296         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_168014, whole genome shotgun sequence.
ACCESSION   DATAIW010000296 DATAIW010000000
VERSION     DATAIW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168014"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..754
                     /locus_tag="VHM48_09225"
     CDS             278..754
                     /locus_tag="VHM48_09225"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2755633.1"
                     /translation="MTEGTRKQPRLVLLCGLPASGKTTLAHEIADAYGAVRLNPDEWE
                     LALGIDPFDEEFQVKLEAEFSRLTERLIALGTSVILEWGFWARSERDEKRDLGRSLGA
                     AVELRFLDVPYDELVRRVVDRHAKGGLAITESHMGRYRGIFQPPTEDELALYDPPL"
     gene            complement(1089..1685)
                     /gene="ruvA"
                     /locus_tag="VHM48_09230"
     CDS             complement(1089..1685)
                     /gene="ruvA"
                     /locus_tag="VHM48_09230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX2755634.1"
                     /translation="MIASVEGKVGAIAFDSLVIEVGGIGYRVFAAPAILATAQPGSTI
                     KLFTYHLVREDQQSLFGFRTTEDLGFFNLLLTVTGVGPKVALAIVGSRPTPDLQLAIM
                     AQDQAVLVSIPGIGRKLADRIIFELKEKVAAAGIAASAAAGSGIGASESEVVGALQAL
                     GYSLSEAREASRIALSDTKIGGTLEDRVKAALRSLLRD"
     gene            complement(1863..3020)
                     /locus_tag="VHM48_09235"
                     /pseudo
     CDS             complement(1863..3020)
                     /locus_tag="VHM48_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          533 a         1091 c         1160 g          505 t
ORIGIN      
        1 gcgcgaggga ctcacgaacc tgaacatcga ctaccgctcg tccgtcgggg agttcccggg
       61 cgcgatgctc ccgatcgtct ccacctgggc ccgcggcgac ggcccattcg ccggagatgc
      121 ccagcggatc aagcagcgcc ggatcagctg atcggccggc gggggtggtg acgggtcagt
      181 ggcaggaatt gcggtcgatg cccgccgggg cccggtcgca ctggacacgc atcttgcctt
      241 gcggattgac ccgatggaca gtgagggggt gcctccgatg accgaaggca cgaggaaaca
      301 accgaggctg gtgttgctgt gcggcctgcc cgcctcgggc aagacgactc tcgcccacga
      361 gattgcggac gcttacgggg cggttcggct caaccccgat gaatgggaac tggccttggg
      421 cattgacccc tttgacgagg agtttcaagt caagctcgaa gcggagtttt cgcgactcac
      481 cgagcggctc atcgcgctgg gaacgtcagt gatcctggaa tggggcttct gggcgcgatc
      541 ggaacgcgac gagaagcggg atctcgggcg atcgctcggc gcagcggtcg aactccgttt
      601 cctcgatgtg ccctacgacg aactggtccg gcgggtcgtg gatcgtcacg ccaagggggg
      661 cctcgcaatc accgagagcc acatgggacg gtatcgaggt atcttccagc cgccaacaga
      721 ggacgagttg gctttgtacg acccgcctct gtaggtcagc actcggtcga gggcgtgggg
      781 atccgtctga accggctccg cccgcggccc gccgcagtcc cgggcaggca aatcgctatc
      841 gccgcgggct cagtccggcc acggccaggg tccgggccgc cgttcgctca aggtccgaca
      901 cgaagccctt gaacgcttgg tacgcctgac cgtccaagcc gatgtagatc tggcacgctc
      961 ggagtcggtc ggcgaactgc ggcacgtcca gcccgatcgt gtcgtagtgc cgagccgccg
     1021 ccccgacgat gtcgatcccg ttggagaacc ccgcaatccc ctgaaggcga gcggcaactt
     1081 gagcctcctc agtcgcggag caggctccgg agtgcggcct tgacccgatc ctcgagcgtg
     1141 ccgccgatct tcgtatcgga cagggcgatc cgcgacgcct cacgcgcctc cgacagcgag
     1201 tagccgagcg cctggagcgc cccgacgacc tcgctctccg aggcgccgat ccccgacccg
     1261 gccgccgcgc tcgccgcgat cccggctgcg gcgaccttct ccttgagctc gaagatgatc
     1321 cggtcggcga gcttcctgcc gatgccgggg atcgacacca ggaccgcctg gtcctgggcc
     1381 atgatggcga gctggaggtc cggcgtcggg cgcgagccga cgatcgcgag ggcgaccttc
     1441 gggccgacac cggtcacggt gagcagcagg ttgaagaagc cgaggtcctc ggtcgtccgg
     1501 aacccgaaga gcgactgctg gtcctcgcgg acgaggtgat acgtgaagag cttgatcgtc
     1561 gacccgggct gagcggtcgc gaggatggcc ggcgcggcga agacgcggta gccgatgccg
     1621 ccgacctcga tgacgagcga gtcgaacgcg atcgcgccga ccttgccctc gaccgaggcg
     1681 atcatgacgc tgggtctccg cgtgacgact cgcgattgtc atacgccgct gacggtggcg
     1741 ttagctcgtc gccggcggtc ggaatcgtgc tgccggcggg tcccgttagc cggtagggac
     1801 gagtgccacc acgtccgggt cgctttgccg gtcgcgctgt ggcaagtgcc accacgtccg
     1861 ggtcagcgct gccagcgcga cggtggctgg tatgggagct cggcgatgag gggctggtcg
     1921 tccggttctc ggcggcgcag cgcgtagaac cgaaggcgtc cgagatccag gtcgacctcc
     1981 gcccggacga gccgatgaag ccaatgctgg tcgacaggca agtggtggcc gaggaccgag
     2041 acccgcccgt tgtccgtcgt ccggcggagg aagacgagcc gaccgatcgg cgcccgatcg
     2101 aaatcgactt cctcgttcgg gaacggacgg cgtgcgggtg cggcctcgat ccgggccgcg
     2161 gcgcgatggc gactggccgt gacataggcg gcggaacgag cctggagatc ctcgagcgtg
     2221 gcgtcggcga agcggttcca gatcttggcc tgccagcgac cgttgaaact ttcgatcgcc
     2281 gcctgaaagc cagattcgcg cggtggcgcg aagaccggcg tgacgccgac ggccaggcag
     2341 gcccgcacga cgccgccgat tgcatcggtc gtgctcgtgc tgccgacgaa caggccgccg
     2401 ttgtcgaact gggcatagcc gggcaggccg acctctcgcc agtgttcggt gagggcaagg
     2461 atcgttcgct ggaccgtgat cgaccgctcg ggccaggcgg cggccagccc gccgtgaagg
     2521 ctgatcccgg tgaggaccga gatgttcgga ccatcctgca gccggaggcc ctcgatcgtg
     2581 tcgaagctgt ccagctcgac ctgctgctgg cggaggtccg gcaggtacca gccggcgggt
     2641 ggagccgggc gtcgtcgtcg ccgccgctcc agaacgtcgt gacgctcgag aatccggccg
     2701 atcgtccgga gcgacggaac cggcccaggc agatcggctt gctccacgag ggcggcccgg
     2761 atcgcccgag ctccgtactc gccgaggatg ctcgcctcgc gcagctccca gcggaggcga
     2821 aggacgacct cttcgacggc cgtgtcggtc cgggtcgtcc gatgtggcgt gtccgggcga
     2881 tcgctccagt cgacccgatc gagacgggtg tcagccgccc gagccaccca gcgctggacg
     2941 gtgccgagac ccaccccgtg ggagcgggcg accgatcgca gcgacgcacc ttctcgaacg
     3001 gccaccacga tcgcccgtcg ccgggtctcg cggcccgagg gagcactgct ttttcgccat
     3061 cgaaaccctc atcagtcgtt ttgaatcgga gggtagacga ctcggacctg gtggcacaca
     3121 tccgacccgg acgtggtggc actcgtccaa tccggctaac ttcgaccgga aatcagcgcg
     3181 ctgacgcgct ccgagcgccg gaatctggtc gaagagtcca ccggcgaata tccggccagg
     3241 tagcacgggc ccgccgagcg gggcgaaggg gcccggcgcg ccgtcactc
//
LOCUS       DATAIW010000297         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_168139, whole genome shotgun sequence.
ACCESSION   DATAIW010000297 DATAIW010000000
VERSION     DATAIW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168139"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VHM48_09240"
     CDS             complement(<1..388)
                     /locus_tag="VHM48_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2755635.1"
                     /translation="MTAGRRFDLVIFDCDGVLVDSERLSIRLDVELLAQLGWPMSEVE
                     VIDRFVGRTDAAMRAEIEAHLGRDIGPEWEAFADRYVRLFAKELRPVVGIVEAVDEIQ
                     AAGYATCVASSGGHEKIRRNLALTGLA"
     gene            complement(385..>2474)
                     /gene="carB"
                     /locus_tag="VHM48_09245"
     CDS             complement(385..>2474)
                     /gene="carB"
                     /locus_tag="VHM48_09245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2755636.1"
                     /translation="LGLLRRGVPEVTIQEATGIAPWFLAEMGRNVGLEGEVRAIRGRL
                     ADPLDAEAATLLATVKRAGFGDRELATMAGVEEEALRDARLALGLRPGYAMVDTCAAE
                     FAAETPYFYATYAAAGSEPEAPPVQRPAALVIGSGPVRIGQGIEFDYCAVQAADTLRQ
                     AGWQAVMINSNPETVSTDFDSSSRLYFEPLDPESVRSVIEAETGPGEELLPAVVAYGG
                     QTPLNLAAPLAAAGVPLLGSDLETIDQAEERTRFSALLDRLGIPQPEGGMAHSIEEAL
                     TLAERIGYPVIVRPSFVIGGLAIDFCYSPEDLVRQLAAATVVDPDRPVRIDRYLEGVE
                     VDVDAVSDGERVLIPGLLEHIERAGVHSGDSVGVFPPGTVSPGDQALIVTTMERIVLA
                     LGVRGLCNAQFIVREDGVYLIEVNPRASRTVPFMSKVTGVPMVELAVRIALGEGLEDL
                     GWPNGLLEPPPFVAVKAPAFSTAKLRGVDPSVGPGMQSTGEVIGISEDPRVALAKALT
                     GASLVPPRRLGPAGGPDDAPLALLSIAERDKAALPRLGSALAAVGYRLAATSGTAAVL
                     EAMGLTAQPVAKFGAAADDTAGEVAILELIASGEVRLVVNTPTPRSGAVRDAAEIRLA
                     ATAEGILCLTAIETAIAAAEALDPAIADRISEVRSLAEWVPNPGRPRRTTGWMDADSG
                     SLEGSAELAATSR"
BASE COUNT          354 a          887 c          895 g          338 t
ORIGIN      
        1 ccgcgaggcc ggtcaaggcc aggttccgcc ggatcttctc gtggccgccg ctcgacgcga
       61 cgcacgtcgc gtagcccgcc gcctggattt cgtcgaccgc ctcgacgatc ccgacgaccg
      121 gtcgcagctc cttcgcgaag aggcgcacat accgatccgc gaaggcctcc cactcgggcc
      181 cgatgtcgcg cccgaggtgg gcctcgatct ctgctcgcat tgcagcgtcc gtgcggccga
      241 cgaaccgatc gatcacctcg acctcgctca tcggccagcc aagctgcgcg agcagctcga
      301 catcgagccg gatggacagc cgctcgctgt cgaccagcac gccgtcgcag tcgaagatga
      361 cgagatcgaa ccgacggccg gcggtcaccg gctcgtggct gccagctcgg ccgatccctc
      421 gagcgatccg gagtccgcgt ccatccagcc ggtcgtgcgg cgaggccggc ccgggttcgg
      481 cacccattcc gcgagcgagc ggacctcgga gatccgatcc gcgatcgccg ggtcgagcgc
      541 ctcggcggcg gcaatggcgg tctcgatcgc ggtcaggcag aggatgccct cggcggtcgc
      601 ggcgagccgg atctcggccg cgtcgcggac ggcgccggag cggggcgtcg gcgtgttgac
      661 gacgaggcgg acctcgcccg aggcgatcag ctcgaggatc gcgacctcgc cggcggtatc
      721 gtcggctgcg gcgccgaact tggccacggg ctgggcggtc aggcccatcg cctcgagcac
      781 agcggccgta ccggatgtcg ccgcaaggcg gtatccgact gctgcgagcg cgcttccgag
      841 ccgtggcagc gccgccttgt ctcgctcggc gatcgagagc agggcgagcg gcgcgtcatc
      901 gggcccgccg gccggcccga gccgccgcgg cgggacgagc gacgcgccag tcagcgcctt
      961 cgccagggcg acccgcgggt cctcgctgat cccgatcacc tcgccggtcg actgcatccc
     1021 gggcccgacc gacgggtcga cgccgcgcag cttggcggtc gagaaggccg gtgccttgac
     1081 cgcgacgaac ggcggcggct ccagcagccc gttgggccag ccgaggtcct cgagtccctc
     1141 accgagcgcg atccggaccg ccagctcgac catcgggacg ccggttacct tgctcatgaa
     1201 gggcacggtc cgagaggcgc gggggttgac ctcgatcagg tagacgccgt cctcgcggac
     1261 gatgaactgg gcgttgcaca ggccgcggac gccgagggcc agcacgatcc gctccatcgt
     1321 cgtcacgatc agggcctggt cgccggggct gacggtgccc ggcgggaaga cgccgacgct
     1381 gtcgccgctg tggacgccgg cccgctcgat gtgctcgaga aggcccggga tcaggacgcg
     1441 ctcgccgtcg ctgaccgcgt cgacatcgac ctcgacgccc tcgaggtagc ggtcgatccg
     1501 gacaggccgg tcgggatcga ccaccgtggc cgcggcgagc tggcggacga ggtcctcggg
     1561 cgagtagcag aagtcgatcg caaggccgcc gatgacgaag ctcggccgga cgatgaccgg
     1621 gtaaccgatc cgctcggcga gggtcaacgc ctcctcgatg ctgtgggcca tcccgccctc
     1681 gggttgggga atcccgagcc ggtccagcag ggcggagaag cgggtccgct cctcggcctg
     1741 gtcgatcgtc tcgaggtcgc tgccgagaag cgggacgccg gcggcggcaa gtggcgcggc
     1801 caggttgagc ggagtctgcc cgccgtaggc gacgacggcc gggaggagct cctcgcccgg
     1861 tccggtctcc gcctcgatca cgctccggac gctctccggg tcgagcggct cgaagtacag
     1921 ccgcgagctc gagtcgaagt cggtcgagac cgtctcggga ttggagttga tcatcacggc
     1981 ctgccagccg gcttggcgga gcgtgtcagc ggcctggacg gcgcagtagt cgaactcgat
     2041 cccctgcccg atccggaccg gtccactgcc gatgacgagc gcggcggggc gctggacggg
     2101 cggcgcctcc ggctccgatc cggcggctgc atacgttgca tagaaatacg gggtctcggc
     2161 cgcgaactcg gccgcgcagg tgtcgaccat tgcataacca ggtcgcagcc caagggccag
     2221 ccgtgcgtct cgcagcgcct cttcttccac gccggccatc gtcgcgagct cgcggtcgcc
     2281 gaagccggcc cgcttgacgg tggcgaggag ggtcgccgcc tcggcgtcca gtggatcggc
     2341 gaggcggcca cggatcgccc gcacctcccc ctccagcccg acgttccggc ccatctcggc
     2401 caggaaccac ggggcgatgc cggtcgcctc ctggatcgtg acctcgggga cgccgcgccg
     2461 gagcaggccg agga
//
LOCUS       DATAIW010000298         5100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_127339, whole genome shotgun sequence.
ACCESSION   DATAIW010000298 DATAIW010000000
VERSION     DATAIW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127339"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHM48_09250"
     CDS             complement(<1..309)
                     /locus_tag="VHM48_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755637.1"
                     /translation="MKKWPVLRIIRAISVLVVLAAIVAQAKVLADAGRFDATRFFAFF
                     TIQSNLVGVAAFSWLIARPDGPRSRGIELLRAGAAVYLTVTFFVVIFLLSGVDVQLQL
                     V"
     gene            446..1834
                     /locus_tag="VHM48_09255"
     CDS             446..1834
                     /locus_tag="VHM48_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX2755638.1"
                     /translation="MVDRIPDTSILTAAELDSIRRPYRAARLLPRKAYHEPTILEWER
                     ANVLRRDWVIVGRVDEAPGPGTYFTTELDGEPLLVVRGRDNELRAFYNVCRHRGTAVV
                     EEPCGKAVRFQCPYHAWIYDLEGKLVRAKHTEDLDDFSFEAYGLQAVGCATWQGFVFL
                     NLDPGAAPLADQLGDLDEHLGRFDFSDLRAARTITYDVAANWKFIAENYSECYHCPPL
                     HPQLNKLTPYDVGGDYAPGGAWQGGWQELVAGAETMALDGGKGSKHGRPPMCGMTTED
                     ERRIFYYVIWPTAFLSIHPDYLLVHRLIPVAPDRTTVICQLLFEPATMARPDFDPTDA
                     IAFWDLTNGQDWHVCEMQQRGTKSSSWVSGRYSNQEASVHAFDLMVAERYAGLPFSAS
                     RTVRERYDVPPPKEAPIGQLDAGARARDAEHEAGRDPHEHDHPTPGRGSHEHTQARTT
                     ARSKATQATTQR"
     gene            1965..3623
                     /locus_tag="VHM48_09260"
     CDS             1965..3623
                     /locus_tag="VHM48_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755639.1"
                     /translation="MPPTASSMPSSQGPVRGPIRDVVLVASGDLRETANRLGWPAQAA
                     LEAALGSALAARGVTVRRGHPIDATTGHGFLSSQRMGMDVFASIDQDTPIVVAEAVWQ
                     YSHHVLAGLRRHRGPILTAANWSGTWPGLVGMLNINASLTKMGRSYSTIWSEDFGDGF
                     AGAALDQWVRTGAIEHDTRHVRDLDASRLAPDAATLGRRLAAELAGRMAILGVFDEGC
                     MGMYNAIIDDELLNPMGLYKERLSQSGLVAEMRLVNGSEAQAAYRWLLARGMKFRFGP
                     DGATELTEDQVLDQLRLYIAAIRIADRFGCDAIGIQYQQGLKDMAPASDLAEGLLNDA
                     DRPPVLHRDDRRELYAGRPLPHFNEVDEGSAVDALVTNRVWSALGQDPATTLHDVRWG
                     APYGDEYVWVFEISGAVPPAHLVGGYAGAVSERQPPVYFGLGGGTIKGVSRPGEIVWS
                     RVFLMDGRLHVDIGRGTVVDLPAAETERRWQATTPQWPIMHAVLHGVTRDQFMARHRA
                     NHVQVAYADGPDAASTALAAKAAMFDAMGVEVHLCGVDEAGLVA"
     gene            complement(3685..4029)
                     /locus_tag="VHM48_09265"
     CDS             complement(3685..4029)
                     /locus_tag="VHM48_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755640.1"
                     /translation="MRATYTRTASGGTSAPGHPSRRLRCDWLDDREPPIEFELPAEAG
                     PLIFGQSELWLASGRSLVVLPLPVASGPARVWDAPAGQIIQWFDPDARIAIGVNRATR
                     ENVATMTGYDLA"
     gene            4162..>5100
                     /locus_tag="VHM48_09270"
     CDS             4162..>5100
                     /locus_tag="VHM48_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2755641.1"
                     /translation="MATPTLIDHDHDPGADAASTRVAALETAAAEPIETAGSRVLVGT
                     ASWTDPTMTAAGVFYPSGADNAEERLAYYASSFPIVEVDATYYALPAERTAQLWAERT
                     PPDFTFDIKAHALMTGQGTETKRLPKAIRAALPPEVQEKTRIYAKDFPAELNDEIWRM
                     FRSALAPLEERGQLGSILLQFPKWVFPSNENRALIDDAVERLAGWRVAVEFRNGSWLN
                     EKNRDRTLAFLSERSIPFVMVDEPQGFKSSVPPDVAVTSPDLAVVRFHGRNAATWEAK
                     NITPAERFRYLYSRDELSEWVPRIRDVASQAKEVHLM"
BASE COUNT          793 a         1846 c         1742 g          719 t
ORIGIN      
        1 gacgagctgg agctgcacgt cgacgcccga cagcaggaag atcacgacga agaaggtcac
       61 ggtcaggtag accgcggcgc cggcccgcaa cagctcgatg ccgcgactcc gcggcccgtc
      121 cgggcgcgcg atgagccacg agaacgcggc gacgccgacg aggttgctct ggatcgtgaa
      181 gaaagcgaag aaccgggttg cgtcgaaccg gccggcgtcg gcgagcacct tggcctgggc
      241 gacgatcgcc gcaaggacga cgagcaccga gattgcgcgg atgatgcgaa ggacgggcca
      301 tttcttcatg cgcctgatct tccgggccgg cggcggtctg agtcaagatc gtgccctgtt
      361 tcaagagtcg tcgcgcgatg cgggacaccg gttcgcctga tggaacgcca gaggcattgg
      421 gttccggccg tgtgacgatc cggcgatggt cgaccgcatc ccggacacct cgatcctgac
      481 tgcagccgag ctcgactcga tccgtcgccc gtaccgcgcc gcccgcctcc tgccgcgcaa
      541 ggcctaccac gagccgacga tcctcgagtg ggagcgggcg aacgtcctcc gccgcgactg
      601 ggtgatcgtc gggcgggtcg acgaggcgcc cgggccgggc acctacttca cgaccgagct
      661 cgacggcgag ccgctcctcg tcgtccgcgg ccgtgacaac gagctccgcg ccttctacaa
      721 cgtctgccgc catcgcggca ccgccgtcgt cgaggagccg tgcggcaagg cggtccgctt
      781 ccagtgcccg taccacgcct ggatctacga cctcgagggc aagctcgtcc gggccaagca
      841 caccgaggac ctcgacgact tcagcttcga ggcatacggc ctccaggctg tcggctgcgc
      901 gacctggcag ggcttcgtct tcctcaacct cgatcccggc gcggcgcccc tcgccgacca
      961 gctgggtgat ctcgatgagc atctcggccg gttcgacttc agcgacctcc gggccgcccg
     1021 gacgatcacc tacgacgtcg cggcgaactg gaagttcatc gccgagaact actcggagtg
     1081 ctatcactgt ccgcccctcc acccccagct caacaagctc accccgtacg acgtcggcgg
     1141 cgactacgcc ccgggcggcg cgtggcaggg tggctggcag gaactcgtcg ccggtgccga
     1201 gacgatggcc ctcgacggcg gcaagggctc gaagcatggc cggccgccga tgtgcgggat
     1261 gacgaccgag gacgagcgcc ggatcttcta ttacgtcatc tggccgaccg cattcctgtc
     1321 gatccacccg gactacctgc tcgtccaccg gctgatccct gtcgcgccgg accggacgac
     1381 cgtgatctgc cagctcctgt tcgagccggc gacgatggcc cgcccggact tcgacccgac
     1441 cgacgcgatc gccttctggg acctgacgaa cggccaggac tggcacgtct gcgagatgca
     1501 gcagcggggg acgaagtcgt cgtcctgggt cagcggccgc tattccaacc aggaggcgtc
     1561 ggtccacgcg ttcgacctga tggtcgccga gcgctacgcc gggctgccgt tcagcgcgtc
     1621 gcgcaccgtc cgcgagcggt acgacgtccc gccgccgaag gaggcgccga tcggccagct
     1681 cgacgcgggc gcccgggcgc gggatgccga gcacgaggcc gggcgcgatc cgcacgagca
     1741 cgaccacccg acgcccggcc gcggctcgca cgagcacacg caggccagga cgaccgcccg
     1801 ctcgaaggcc acgcaggcga cgacgcagcg ctgaccggcg tcgtccggcc gctccggcgc
     1861 gctcggacgc ccggcctcgc agccctgttc cgcccgacga gcgccgtccg tcgggctgca
     1921 tgattgcggt ccgtccgtcc gcgagccaat cgggaggtcc gtcgatgcca cccaccgcgt
     1981 cctcgatgcc gagcagccag gggcccgtcc gcggcccgat ccgggacgtc gtcctcgtcg
     2041 cgagtggtga cctccgcgag acggcgaacc gcctcggctg gcccgcccag gccgcgctcg
     2101 aggcggccct cgggtccgcc ctcgccgcgc gcggggtgac ggttcggcgc ggccacccga
     2161 tcgacgcgac gaccggccac ggcttcctgt cgagccagcg gatggggatg gacgtcttcg
     2221 cctcgatcga tcaggacacg ccgatcgtcg tcgccgaggc ggtctggcag tacagccatc
     2281 acgtcctcgc cgggttgcgc cgccatcgcg gccccatcct gacggcggcc aactggagcg
     2341 ggacctggcc gggtctcgtc gggatgctca acatcaacgc ctccctgacg aagatgggcc
     2401 gctcctacag cacgatctgg agcgaggact tcggcgacgg gttcgccggc gcggcgctcg
     2461 accaatgggt ccggacgggc gcgatcgaac acgacacgcg gcatgtgcgc gacctcgacg
     2521 ccagccgcct cgctccggac gccgcgaccc tcggtcgacg attggccgcc gagctcgccg
     2581 gccggatggc gatcctcggc gtcttcgacg aaggctgcat ggggatgtac aacgcgatca
     2641 tcgacgacga gctcctcaac ccgatgggcc tctacaagga gcggctcagc cagtccgggc
     2701 tcgtcgccga gatgcgcctc gtcaacggat cggaggcgca ggccgcctac cgctggctgc
     2761 tcgcgcgagg catgaagttc cggttcggac ccgacggcgc caccgagctg accgaggacc
     2821 aggtgctcga ccagctccgc ctctacatcg cggcgatccg catcgcggac cggttcggct
     2881 gcgatgcgat cgggatccag taccagcagg gcctcaagga catggcgccc gcgtccgacc
     2941 tggcggaggg attgctcaac gacgccgatc ggccgcccgt cctccatcgc gacgatcggc
     3001 gcgagctcta cgccggtcga ccgctgcccc atttcaacga ggtcgacgag ggctcggcgg
     3061 tcgacgccct cgtcacgaac cgtgtctggt cggcgctcgg ccaggacccg gcgacgacgc
     3121 tccacgacgt gcgctggggt gcgccgtacg gcgacgagta cgtgtgggtg ttcgagatct
     3181 cgggagccgt cccgccggcg cacctcgtcg gcggctatgc cggcgcagtc agcgagcgcc
     3241 agccgccggt ctacttcggt ctcggcggcg ggacgatcaa gggcgtcagc cggccgggcg
     3301 agatcgtctg gagtcgggtc ttcctgatgg acggccggct gcacgtcgac atcggtcggg
     3361 gcacggtcgt cgacctgccg gcagccgaga ccgagcggcg ctggcaggcc acgacgccgc
     3421 agtggccgat catgcacgcg gtcctccacg gcgtgacgcg cgaccagttc atggcccgtc
     3481 accgggcgaa ccacgtccag gtcgcctatg cggacgggcc ggacgccgcg tcgacggccc
     3541 tcgccgccaa ggccgcgatg ttcgatgcga tgggcgtcga ggtccatctc tgcggcgtcg
     3601 acgaggcggg cctggtcgca tgaccgacct gctcctcgga atcgacgtcg ggatcgcggg
     3661 ctcgtagggc ggatctgggc cgtctcaggc caggtcgtac ccggtcatcg tcgcgacgtt
     3721 ctcccgcgtc gcgcgattga cgccgatcgc gatccgggca tccgggtcga accactggat
     3781 gatctggccg gccggagcat cccacacccg agccgggccg gacgccaccg gcagcggcag
     3841 gacgacgagc gaccggccgc tcgccagcca cagctccgac tgtccgaaga tcagcgggcc
     3901 cgcctcggca ggaagctcga attcgatcgg tggttcgcga tcgtcgagcc aatcgcaccg
     3961 cagacgccga ctgggatgac ccggggcgct cgttcccccg gacgcggtcc gggtataggt
     4021 ggcgcgcacg tcgacggtcg accagagcga cgtcccgtcc gggactcagg gggactgacg
     4081 caggccgccc ggcacccgtc cggaacggct cggcccgcgt tgttgacgac ttccggcagg
     4141 atcgcgagat aggctgagtc gatggcgaca ccgaccctga tcgaccacga tcacgatccc
     4201 ggggcagacg cggcgtccac gcgcgtcgcc gcgctcgaga ccgccgccgc cgagccgatc
     4261 gagacggccg gttcgcgggt cctcgtcggg accgcgtcgt ggaccgaccc gacgatgacc
     4321 gcggccggcg tgttctaccc gagcggcgcc gacaacgccg aggagcggct cgcttattac
     4381 gcgtcgtcgt tcccgatcgt cgaggtcgac gcgacctact acgccctgcc ggccgagcgg
     4441 acggctcaac tctgggccga gcgcaccccg ccggacttca ccttcgacat caaggcccac
     4501 gccttgatga ccgggcaggg gaccgagacg aagcggctcc cgaaggcgat ccgcgcagcc
     4561 ctcccgcccg aggtgcagga gaagacccgg atctacgcca aggacttccc ggccgagctc
     4621 aacgacgaga tctggcggat gttccgctcg gccctcgccc ccttggagga gcgtggccag
     4681 ctcggctcga tcctcctcca gttcccgaaa tgggtgttcc cctcgaacga gaaccgggcc
     4741 ctgatcgacg acgcggtcga acgcctggcg ggctggcggg tcgcggtcga gttccgcaac
     4801 ggctcatggc tgaacgagaa gaaccgcgac cggacgctgg cgttcctgtc ggagcgctcg
     4861 atcccgttcg tcatggtcga cgagccgcag ggcttcaaga gcagcgtccc gcccgacgtc
     4921 gcggtgacct cgccggatct cgcggttgtc cgcttccacg gccgcaacgc cgcgacctgg
     4981 gaggcgaaga acatcactcc ggcggagcgc ttccgctacc tctactcgcg cgacgagctc
     5041 tcggaatggg ttccgcggat ccgcgacgtc gcctcccagg cgaaggaggt acacctgatg
//
LOCUS       DATAIW010000299         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_168272, whole genome shotgun sequence.
ACCESSION   DATAIW010000299 DATAIW010000000
VERSION     DATAIW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168272"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VHM48_09275"
     CDS             complement(<1..180)
                     /locus_tag="VHM48_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755642.1"
                     /translation="MEIGEMDVDQLIECFTVAPRCTRDEIQVVPPSCSVIQAPAPGES
                     FGRFRNGDHRAGGMSS"
     gene            422..811
                     /locus_tag="VHM48_09280"
     CDS             422..811
                     /locus_tag="VHM48_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755643.1"
                     /translation="MTAGVRFVGAGDRVVVTDTGDTFDPVAETWGTTTKCEGAGAGTV
                     WTGRHLLGVAAAWDSRGGGNCVQLPPSPKRQPPFDDTNGREFPVGIWTGRQYITWSGG
                     TGADIVWVPKDGAVFTPKDDLGPAGAP"
     gene            complement(808..2967)
                     /locus_tag="VHM48_09285"
     CDS             complement(808..2967)
                     /locus_tag="VHM48_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2755644.1"
                     /translation="MSAYSPDPRDLEAPRAAAAGRDPLLARAARYARWDGTQQVPALD
                     ADEIIDALADDVMAEGDLAEALRRLVERGLRTSDPTRPDLAGLKELRERLRRRRDELQ
                     ERYQLRDVLADVRQELDEIVAQERAGIERRLDEAATPSKPAPPDASSTSSSNATSADA
                     ALRAMLRDAAARRIDQLDALPRDVGGRIRKLEEYDFMEPAARDRFNTLTEKLRKQTLD
                     RFVDGLSEAIQGTTPEELDANRDMVRDLNSLLEERLEGREPAQDQVDDFLAKHGRFFP
                     GARTLDEIVDQLTQRMAAMQSLLRSMTPQQRAELQSMMDALLRDDRLRWDLARLASNM
                     DQLMPDGVGESYDFSGEEPLGLEPALDRIGQLQALDALEDELATIESPSDLEGLDRDG
                     VRNLLGDDAARDLDALDALARQLEEAGYLTTKGNRLELTPRGSRRIGQKVLDDLFAKL
                     SRDAFGGHRIDRTGRGGEREETTKPYEFGDPFHLDIRGTIENALRRPENVPAERLARG
                     ESGVHLSASDFEVFRTEQLTKTSTVLLVDMSRSMLLRGCFLAAKKVAVALDTLIRTQF
                     PHDDLTVIGFAYYARELRPEALAELTWHGYEYGTNLQHGLMLARRILARQRGGNRQIV
                     VITDGEPTAHFENGQVEFSYPPTRRTIQETLREVQRCTRDGITINTFMLERSRALAEF
                     VALMTRLNRGRAFYATPEHLGEYVLVDFVSGRTRGVG"
     gene            complement(3167..4612)
                     /locus_tag="VHM48_09290"
     CDS             complement(3167..4612)
                     /locus_tag="VHM48_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755645.1"
                     /translation="MSSPSVDRKRRPETLGELRAAGWSSRTVKQELRSNLLARLAADR
                     PIVSGIVGYDDSVLPAIENAVVAGQDLVLLGERGQAKTRLARLLVGLLDDAIPVVRGG
                     ELNDDPYAPISPSGKTIVAEQGDATPIDWLPRDRRYSEKLATPDITIADLIGEVDPIR
                     VAEGRYLSDELTLHYGLIPRSNRGIFAINELPDLAERIQVGLLNILEERDVQIRGFTV
                     RLPLDLFVIASANPEDYTSRGRIITPLKDRLGSQVRTHYPRTLDHELAIVRQEKQRFI
                     GGEGEPVIAVPGFMEEVLAELTHLARRSPEISQRSGVSVRVTVANTEVLEAASLRRAV
                     RLGESVAAPRVSDLGAVVASTVGKIELESVGDDAPEERIVERLITKALFATFGRLVDI
                     DDLDDAVLSFEDGLVVETGDRVPSREYVRWMAEIPGLHEAVRRLGTFDVTDGAEEPAV
                     VASAVEFLLEGLHLSRRINKDRSGAGTVYRR"
     gene            complement(4773..5672)
                     /locus_tag="VHM48_09295"
     CDS             complement(4773..5672)
                     /locus_tag="VHM48_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755646.1"
                     /translation="MNPDDRLAFLRASTAPPRRSIVELIATGVLDADLAALAWILVEA
                     RLPLIVAGLPQGAGKTTVLEALLDFLPPTTRRIELAGETEDFGWLPEAEALGWSREAR
                     VALAAIPITPASACLVAAEISEHLPIYTWGRAARTLVRAASLGYGIAATLHADRLEEV
                     HALLRGRPIGLTDDELTYLGLILVVRAERGSDGHVRRRVVAAHYARPVGRDEHGHTQR
                     LPPAVLAHRDPTTDRLDHFAWGVMPELAIRLGRKSGDLEREQAERSAILAGLADEGAI
                     DLDTLRATIASRRASTRADAHVH"
     gene            5721..>6307
                     /locus_tag="VHM48_09300"
     CDS             5721..>6307
                     /locus_tag="VHM48_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755647.1"
                     /translation="MARSPRSAKPAKSAPIDLAASSIEIVPLTPDRWADVTALFDEGG
                     DPKTCWCMNWRVRSRDWSFATAGESRDGLRALVDDGRDPSPGLLAYAGGRAVGWVSVA
                     PREDYGRLVNSRVRPKIDDVPVWSIVCFVVSRTVRGQGLTTRLLDAAMEYARAHAAPG
                     LEAYPVDAGDGRVPAALGYTGHLSTFLAAGFRVVH"
BASE COUNT          859 a         2174 c         2333 g          941 t
ORIGIN      
        1 gctggacatg ccgccggcgc ggtggtcacc gttgcgaaat cgaccgaacg attcgcctgg
       61 cgcaggcgcc tggatgaccg agcacgaggg aggcacgacc tggatctcgt cgcgcgtgca
      121 gcgaggggcg accgtgaagc attcgatgag ctggtcgaca tccatctcgc cgatctccat
      181 cggctcgcgc tcatcgttgc cggaccggac cgggccgagg acgtgaccca ggaggcgttc
      241 ctggccgcct ggcgcgagct gccccgcctt cgcgaccccg atcggtttct gccgtggatc
      301 cggcggatcc tcgtcaaccg cgctcgcgac ggccagcgat ccgaacgcgg gcctggacgc
      361 gtgggcagtc ctcgacccgg cgaccggtca gtggcatcac ggcctggtcc cccttgaccc
      421 gatgaccgcc ggggttcggt tcgtcggcgc cggcgatcgg gtcgtcgtga cggacactgg
      481 cgacaccttc gatcccgtcg cggagacgtg gggcacgact acgaaatgcg agggtgccgg
      541 cgcgggcacc gtctggaccg ggcgccacct cctcggcgtc gccgcagcct gggactcccg
      601 tggcggcggc aactgtgtcc agctcccgcc gtcaccgaag cgacagccgc cgttcgatga
      661 caccaacggc cgcgagtttc cggttgggat ctggacggga cggcaataca tcacgtggag
      721 cggcggcacc ggcgcggaca tcgtctgggt tccgaaggac ggcgcggtgt tcacgccgaa
      781 ggacgacctc ggacctgccg gagcccctta gcccaccccg cgtgtccggc cggagacgaa
      841 atcgaccagg acgtactcgc cgaggtgctc cggtgtcgcg tagaaggccc ggccacggtt
      901 caggcgggtc atcagcgcga cgaactcggc gagcgcgcgc gaccgctcga gcatgaacgt
      961 gttgatcgtg attccgtcgc gggtacagcg ctggacctcg cggagggtct cctggatcgt
     1021 ccggcgcgtc ggcgggtagc tgaactcgac ctggccgttc tcgaaatgag cggtcggctc
     1081 gccgtcggtg atgaccacga tctggcggtt gccgccgcgc tgccgggcga ggatccggcg
     1141 ggccagcatc agcccgtgct ggaggttcgt gccgtactcg tagccgtgcc aggtcagctc
     1201 ggcaagcgcc tccggacgga gctcgcgggc gtagtaggcg aagccgatca cggtcaggtc
     1261 gtcgtgcggg aactgggtcc ggatgagggt gtcgagggcg acggcgacct tcttcgccgc
     1321 gaggaaacac ccgcgcagca gcatcgagcg gctcatgtcg acgagcagca ccgtggaggt
     1381 cttcgtcagc tgctcggtcc ggaacacctc gaagtccgaa gcggagagat ggacgccgga
     1441 ctcgccgcgc gccagccgct cggcgggcac gttctccggg cggcggaggg cgttctcgat
     1501 cgtgccccgg atgtcgagat ggaacgggtc gccgaactcg tacggcttgg tcgtctcctc
     1561 gcgctcccct ccccgcccgg tgcggtcgat ccggtggccg ccgaacgcgt cgcgtgacag
     1621 cttcgcgaac aggtcgtcga ggaccttctg gccgatccgc cggctgccgc gcggtgtcag
     1681 ctccagccga ttgcccttgg tcgtcaggta gccggcttcc tcgagctggc gagcgagggc
     1741 gtcgagcgcg tcgaggtcgc gggcggcgtc gtcgcccagc aggttgcgga cgccatcgcg
     1801 gtcgagccct tcgagatcgg atggcgactc gatcgtggcg agctcgtcct cgagggcgtc
     1861 gagcgcctgg agctggccga tccgatcgag tgccggctcg agtccgagcg gctcctcgcc
     1921 gctgaagtcg tagctctcgc cgaccccgtc aggcatcagc tggtccatgt tcgacgccag
     1981 ccgagcgagg tcccagcgca gccgatcatc gcggagcaac gcgtccatca tcgactgcag
     2041 ctccgcccgc tgctgggggg tcatcgagcg cagcagcgac tgcatcgccg ccatccgctg
     2101 ggtcagctgg tccacgatct cgtcgagggt tcgggcgccc gggaagaagc ggccgtgctt
     2161 ggcgaggaag tcgtcgacct ggtcctgggc cggctcgcgg ccctcgaggc gttcctcgag
     2221 caggctgttc aggtcgcgaa ccatgtcgcg gttggcgtcg agctcctccg gcgtcgtccc
     2281 ctggattgcc tcggagaggc cgtcgacgaa gcggtcgagg gtctgcttcc ggagcttctc
     2341 agtgagcgtg ttgaaccggt cgcgggcggc cggctccatg aagtcgtact cctcgagctt
     2401 ccggatccgg ccgccgacgt cgcgcggcag cgcgtcgagc tggtcgatcc ggcgggccgc
     2461 ggcgtcgcgg agcatcgccc tcagagcggc gtcggctgac gtcgcgttgg atgacgaggt
     2521 tgacgacgcg tcgggcgggg caggtttcga gggcgtcgcc gcctcgtcca ggcggcgctc
     2581 gatgccggct cgttcctggg cgacgatctc gtcgagctcc tggcggacgt cggcgaggac
     2641 gtcgcgcagc tggtagcgct cctggagctc gtcgcgccgg cggcggagac gctcgcggag
     2701 ctccttgagg ccggcgagat cgggccgcgt cggatcggac gtccgcaagc cgcgctccac
     2761 caggcgacgg agcgcctcgg cgaggtcgcc ctcggccatc acgtcgtcgg cgagcgcgtc
     2821 gatgatctcg tcggcgtcga gggccgggac ctgctgcgtc ccatcccagc gggcatagcg
     2881 ggcggctcgg gcgaggagcg ggtcgcgccc ggccgcggcc gcgcggggcg cctcgagatc
     2941 gcgcgggtct ggggagtagg cgctcatcgc ggcgtcgccc gcggctgatg ctgcgcaggc
     3001 attgtcttcg aagcgaggcc cacttcggag gcctccagcc ggctgttcga tgccggaccg
     3061 gcatcgaacc gaggcgaaac gggccgattc cgcggcgtgg cggcgcgttc cgcctgaatt
     3121 cgatgcttcc ggagcatcgc accggacgcg tcggcgccgc gcgcgttcat cgccggtaga
     3181 ccgtgccggc tcccgagcga tccttgttga tccggcgtga caggtgcagc ccttcgagca
     3241 ggaactcgac cgccgacgcg acgaccgccg gctcctccgc cccgtcggtc acatcgaacg
     3301 tgccgagccg gcggaccgcc tcgtggagcc ccgggatctc ggccatccag cggacatact
     3361 cgcgcgacgg gacgcgatcg ccggtctcga ccacgagccc gtcctcgaag gagaggacgg
     3421 cgtcgtcgag atcgtcgatg tcgacgagcc ggccgaacgt cgcgaacagc gccttggtga
     3481 tcagtcgctc gacgatccgc tcctcggggg cgtcgtcgcc gaccgactcg agctcgatct
     3541 tgccgacggt gctggcgacg acggcgccaa ggtccgagac gcgcggcgcg gccacggatt
     3601 cgccgaggcg gacggcccgt cgcagcgacg ccgcctcgag gacctcggtg ttggcgaccg
     3661 tgacccggac gctgacaccg gaccgctggc tgatctcggg tgagcgacgg gcgaggtggg
     3721 tcagctcggc gagaacctcc tccatgaaac ccggcacggc gatgacgggc tcgccctcgc
     3781 cgccgatgaa ccgttgcttc tcctggcgaa cgatcgcgag ctcatgatcg agggtccgcg
     3841 ggtagtgggt ccggacctgc gagccgagac ggtccttgag cggggtgatg atccggccgc
     3901 gcgacgtgta gtcctcgggg ttggcgctgg cgatcacgaa caggtcgagc ggcaggcgga
     3961 ccgtgaatcc gcggatctgg acgtcgcgct cctcgaggat gttcaggagg ccgacctgga
     4021 tccgttcggc caggtccggc agctcgttga tcgcgaagat cccccggttc gagcgcggga
     4081 tcaggccgta gtggagggtc agctcgtcgc tcaggtagcg gccctcggcg acccggatcg
     4141 ggtcgacctc gccgatcagg tcggcgatcg tgatgtccgg cgtcgcgagc ttctcgctgt
     4201 agcgccggtc acggggcagc cagtcgatcg gcgtggcgtc gccctgctcg gcgacgatgg
     4261 tcttgccgct cgggctgatc ggcgcgtacg ggtcatcgtt gagctcaccg ccgcggacga
     4321 ccgggatcgc gtcgtcgagc agccccacga gcaggcgggc gagccgggtc ttggcctggc
     4381 cgcgttcgcc gaggagcacg aggtcctggc cggcgacgac cgcgttctcg atcgcgggca
     4441 ggaccgagtc gtcgtagccg acgatcccgc tgacgatcgg ccgatcagcc gccagccggg
     4501 cgaggaggtt ggaccggagc tcctgcttga ccgtccggct cgaccagccc gcagcacgca
     4561 gctccccgag ggtctcgggt cggcgtttgc gatcgacgga cggggatgac acgggcggcg
     4621 agcgtacgac cggctcgccg gaccgtcaaa cgccgggccg ggatcgccgc aggtcaaccg
     4681 ccgagggtgg cgacgatcgc ggacccggct gtcggccgcg gcgccgcgag caccccggcg
     4741 atgacctccg ccatcggggg gatcgcgagg cgtcagtgga cgtgagcgtc cgcgcgagtt
     4801 gaggcccgcc gcgaggcgat cgtggcccgg agcgtgtcca gatcgatcgc cccttcgtcc
     4861 gccagtccgg caaggatcgc cgaccgttcc gcctgctcgc gttcgaggtc gccggacttc
     4921 cggccgagcc ggatcgccag ctcgggcatg acgccccacg cgaagtggtc gaggcggtcg
     4981 gtggtcgggt cgcggtgggc caggaccgcc ggcggaaggc gctgggtgtg gccgtgctcg
     5041 tcgcggccga ccggccgggc gtagtgggcc gcgaccaccc gccgccggac gtggccgtcg
     5101 gaaccccgct ccgcccggac gacgaggatc agcccgaggt aggtgagctc atcgtcggtc
     5161 aacccgatcg gccggccgcg gaggagcgcg tggacctcct cgagccggtc tgcgtggagg
     5221 gtcgccgcga tcccgtagcc gagcgacgcc gcccgcacca gcgtccgggc tgcccggccc
     5281 caggtgtaga tcgggaggtg ctccgagatc tccgcggcca cgaggcaggc ggaggccggc
     5341 gtgatcggga tcgcggccag cgcgactcgc gcctcgcggg accagccgag cgcctcggcc
     5401 tccggcaacc agccgaagtc ctcggtctcg cccgcgagct cgatccggcg ggtcgtcggc
     5461 ggcaggaagt ccaatagcgc ctcgaggacc gtcgtcttgc cggctccctg ggggaggccg
     5521 gcgacgatga gcggcaggcg cgcctcgacg aggatccagg cgagggccgc gagatccgcg
     5581 tcgaggacgc ccgtcgcgat cagctccacg atcgagcggc gcggcggagc ggtcgacgcg
     5641 cgcaggaacg cgaggcggtc gtcgggattc acttgccgat catgacaggc gctggccgat
     5701 cggcggtcca cactgccggc atggcccgct ccccacgatc cgccaagcct gcgaaatccg
     5761 ccccgatcga tctcgccgct tcgtcgatcg agatcgtgcc gctgacgccc gaccgctggg
     5821 ccgacgtgac cgcgctgttc gacgaaggcg gcgacccgaa gacctgctgg tgcatgaact
     5881 ggagggtccg ctcgagggac tggtcgttcg ccactgccgg tgagagccgc gacgggctcc
     5941 gcgcgctcgt ggacgacggg cgcgaccctt cgcccggcct gctggcgtat gccggtggcc
     6001 gggccgtcgg ctgggtcagc gtggcgccgc gcgaggatta cgggcggctc gtcaactcgc
     6061 gcgtccgccc gaagatcgac gacgtgcccg tctggtcgat cgtgtgcttc gtcgtctccc
     6121 ggaccgtccg cggccagggc ctgacgacgc gcctcctcga cgccgcaatg gagtacgccc
     6181 gggcgcacgc cgccccgggt ctcgaggcgt atcccgtcga cgcgggcgac ggccgggttc
     6241 ccgccgccct cggctacacc ggccacctgt cgaccttcct ggcggccggg ttccgggtcg
     6301 tccacga
//
LOCUS       DATAIW010000300         7988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_48926, whole genome shotgun sequence.
ACCESSION   DATAIW010000300 DATAIW010000000
VERSION     DATAIW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48926"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..1090)
                     /locus_tag="VHM48_09305"
     CDS             complement(545..1090)
                     /locus_tag="VHM48_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX2755648.1"
                     /translation="MTTFIALLRGINVGGNRRVPMADLRAALDEIGLDEVRTYIVSGN
                     VLFRTTRTDPVALTREIEAAIEARFGFALAVVLRSAAEMRSVVAADPLPEGSAGDPAQ
                     RYAIFLSADPAPERLAAIDRAAVAPDVFVAGDRVIHAWYRNGLQQSKLAGQLTDRGLG
                     VTATARNWNTVRKLVELAGDG"
     gene            1192..1431
                     /locus_tag="VHM48_09310"
     CDS             1192..1431
                     /locus_tag="VHM48_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX2755649.1"
                     /translation="MTEPTGRIAWIAIAPVKSMALVFLDRAELELEGIAGDRAFAVID
                     GTGRLVNGKRAGSLATVVVEHDQATGDLRPRHAVS"
     gene            1418..1978
                     /locus_tag="VHM48_09315"
     CDS             1418..1978
                     /locus_tag="VHM48_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755650.1"
                     /translation="MRFPDGTLAAGRPTIGDPIEAIFFGEPRPARAVDGPWSDALAAW
                     SGKDLRLVAMGRGEGPDRGPTTTLLSTAALTELAAAGGAAAPLDRRRFRMTFGVDGVA
                     PYAEDGWLGRDVRIGGAVVRVAGNVGRCAVTTHDPDTGRPSFDTLHVLNHSRGHLAAS
                     EPLPFGVWAEVVTPGAVALADPVEVT"
     gene            1975..2898
                     /locus_tag="VHM48_09320"
     CDS             1975..2898
                     /locus_tag="VHM48_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2755651.1"
                     /translation="MNQAPSLRLGLNLPYTEGQMDGATPRWTDIRAMAVAAEVVGFDA
                     VWISDHVGFGDPASPEPGRWSGAWDCWTLLAALAAVTTRVHLGTYVLCTAFRNPALLA
                     KMAETLDEVSGGRVILGLGAGWNEIEFSSYGYPYAERFGRFEDSLRIIAAMLRTGRAT
                     YNGRWERAADARIEPRGPRAAGLPIMVGAGGPRMLRLTAELADRWNGGMGSVAGTIET
                     LAALNEACRAVGRDPASIERSVEVLVRPGPAPADEQGEPNELRGSPIELAASLLAYRA
                     LGVGELQVQLRPNTLAAIDAFAPVIAAIRRG"
     gene            3282..4829
                     /locus_tag="VHM48_09325"
     CDS             3282..4829
                     /locus_tag="VHM48_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2755652.1"
                     /translation="MTTFLDQTPDLDLDARPGATPALDELAPTREDVAAVVEHDPTLT
                     LVAPVKKTAVAKAARDDEPEIAEHDDLLDIEDSTRLYLREIARVPLLTAEEEVMLAKT
                     MELGLRITKDPGLAVLDLHVWTANASEPTARAKHPRYAMKFADLVARIVRSAIEADEA
                     SEFLVTAPRFGLTESIEGAVGEAAELLERARNMRSVYNERLDAETFLTTLAWIHGAAS
                     RPVVRENPAMIAMRTWTRDAVGLPAVRRWLDGGHDTDAIETMAPTLVATGLTAREHLT
                     SANLRLVVANAKKYINRGMSLLDLVQEGNAGLMRGVDKFEWERGFKFSTYATWWIRQA
                     IQRGLADQSRTIRIPVHMVETMNRVTRTSRELTVELGREPTNAEIAALLSEDPKTAIT
                     TEKVEEVRSYGRNPVSLETPIGDESDTELGSLIEDPDAISPLEAVSDTMLREQVARVL
                     DSLEGREERVIRLRFGLDDGRPRTLEEVGHEFGLTRERIRQIEAHALRKLRHPSRSRK
                     LRDYVTD"
     gene            complement(4969..5496)
                     /locus_tag="VHM48_09330"
     CDS             complement(4969..5496)
                     /locus_tag="VHM48_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2755653.1"
                     /translation="MTTSAGSDSDPARESLDRALAGHGPYELFACDPALADTAAFCAA
                     YGFDPADSANTIVVIGKSDPPVYAACVVLATHRLDVNRVVKQRLRTRKASFASPDETR
                     ILTGMEIGGVTAFGLPNDLPLWVDGAVMDRERIVLGGGSRSWKVIAPPAILRTLPNVE
                     VVADLAAPRPADPAT"
     gene            complement(5547..6512)
                     /locus_tag="VHM48_09335"
     CDS             complement(5547..6512)
                     /locus_tag="VHM48_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755654.1"
                     /translation="MTIHRGSVIAATWLIGLGLVFLVRQALALDWGEAWPMLLILGGV
                     ASFVSRAFGRPRGIAGIWSFTWPVLWIALGVVLLMSTTGNLGRGPVDLISDGWPWVLV
                     GLGVWFVIGAILPFGGSPMEELVIPLAGVADAAVRIRFGAGELAVGAAAAGNLVDGHF
                     SGGVVRRDRGHGRVDLEQDTTYGLPWLDGRSDWSVGLATGVPLDLRLDTGAARARLDL
                     TDLKLRNLELHTGASETRIRLPRAAGASTVRAETGAAALIIEVPAEVAARIRSRMAIG
                     SSQVDQSRFPRTATGYESPDYATAANRVDLDLQGGVGSIRVIGVA"
     gene            complement(6509..7189)
                     /locus_tag="VHM48_09340"
     CDS             complement(6509..7189)
                     /locus_tag="VHM48_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2755655.1"
                     /translation="MNTHLYRARDDRMLAGVAGGLADLWDADPSLVRIIWALLVPLTG
                     GIALVVYIVMAIVVPEEDGSRSWGSRAPAWDAPTGSPSSAPAGGAPAPTAETASVTDA
                     AGLSPAQPASAAATIPASSGPSATDWQASRFEARDMRRAARHEARAARRASRGYDGRS
                     GAVVVGVLFILVGAWSLVRQYLPEIDWDWFWPLVLVGVGVLVLVLAVRPRSDPSKPAD
                     ANGGSTRS"
     gene            complement(7186..>7988)
                     /locus_tag="VHM48_09345"
     CDS             complement(7186..>7988)
                     /locus_tag="VHM48_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755656.1"
                     /translation="SRRTAADGPSNDPDRWSCWSVGGAAIASGHLWIGALSDCAVSGG
                     PTDQQLQRSGSFDGPSAAVRLDLTCGSLTVATSTGANWALDAAYRGPAPIVDSSSSRL
                     DLRVSGGGSARRNDWTISLPSAVGVLSITANAATATLQFPDGHVASLDVDMNAGDLRI
                     DATHGAVDVVDLSMNAGRIRLDAGSSVLGGDVSVNAGAFDLCVPDGVGLRLEVTNQLT
                     FVHNLAARGLSRSGDTWTRPAAAGAPIVTLSIDGGAASFSLNPVGGCR"
BASE COUNT         1231 a         2869 c         2774 g         1114 t
ORIGIN      
        1 aggtcaggcc gtcacggtgg aagaagtatt ccttgccgtc ctcgcccgag atgaagccga
       61 aaccgcgctc ggaaacgacc ttcttgatgg tgccggtggt cacgatgacc tccaatgtct
      121 tctcgaacgg gttgcttcag acgcacggtc agttcgagaa gtcggtcgac cacacgggag
      181 catcaccaca gcggcgattg aggaagggta gcacggatat cccgccggcc cgatctgcgc
      241 tgaggattct ggactcgatc gaacctgaca ggtcctgacc gcgccggcgt catgatcggt
      301 cgtgactgga tcaccggctc gcgcacgacg atctggcggc gccgatcatc gtcaacggac
      361 tgtggggcta ctcgctgacg atccacctgc tccacgtcgt cgaccacgcc gtccaatcct
      421 tcaccgaggc cgcggtcctg ctgacggccg ccggtctctc gccgggcgac ctcgacttcc
      481 tcgatttcca cgacacgccg gagcggcgag ctgccgcgcg cggctgatcc gtccagccgc
      541 tcggtcagcc gtcgcccgcc aactcgacga gcttgcgaac ggtgttccag ttcctcgcgg
      601 tggcggtgac gccgagcccg cggtccgtga gctgaccggc gagcttcgac tgctggagcc
      661 cgttccggta ccaggcgtgg atcacccggt cgccggcaac gaacacgtcc ggcgcgaccg
      721 ccgcccggtc gatcgcggcc aggcgttccg gcgccgggtc cgccgacagg aagatcgcgt
      781 agcgctgagc cggatcgcca gccgagccct cgggcaacgg gtcggccgcg acgaccgatc
      841 gcatctccgc cgcactccgc aggacgacgg ccagcgcgaa cccgaaccgc gcctcgatcg
      901 ccgcctcgat ctccctggtc aacgccaccg gatccgtccg cgtcgtccgg aacaggacgt
      961 tgccgctgac gatgtacgtc cggacctcgt cgaggccgat ctcgtcgagg gccgcgcgga
     1021 ggtcggccat cgggacccgc cgattgccac cgacgttgat cccgcggagg agggcgatga
     1081 aggtcgtcat cgccggatct tcgcccgtcg cggtccccct gtcacaggca ggcccgcccg
     1141 aaccggatgc cggcgacccc gcagtcggcg ccgatcgacg atcatccgcg aatgaccgaa
     1201 cccaccgggc gcatcgcctg gatcgcgatc gcgccggtga agtcgatggc gctcgtgttc
     1261 ctcgaccggg cagagctcga gcttgaaggc atcgcgggcg accgcgcctt cgcggtcatc
     1321 gacgggaccg gtcgcctcgt gaacggcaag cgcgccgggt cgcttgcgac cgtggtcgtc
     1381 gagcacgatc aggcgaccgg cgacctgcga cctcgccatg cggtttcctg acggcaccct
     1441 ggcggccggt cggccgacga tcggcgaccc gatcgaggcg atcttcttcg gcgagccgcg
     1501 acctgctcgc gccgtcgacg ggccctggag cgacgccctc gcggcctggt cggggaagga
     1561 cctgcggctc gtggcgatgg gccggggcga gggcccggat cgagggccga cgacgacgct
     1621 cctctcgacc gccgccctga ccgagcttgc ggccgccggc ggagcggcag caccgctcga
     1681 ccgtcgccgc ttccggatga ccttcggggt cgatggggtc gcgccctacg ccgaggacgg
     1741 ctggctcggc cgcgatgtcc ggatcggggg cgccgtcgtg cgtgtcgcgg gcaacgtcgg
     1801 tcgctgtgcg gtcacgaccc acgaccccga caccggccgg ccgtcgttcg acaccctcca
     1861 cgtcctcaac cacagtcgcg gccatctggc agcaagcgag cccctcccct tcggggtctg
     1921 ggcggaggtc gtcaccccgg gcgccgtggc cctcgcagat cccgtggagg tcacgtgaac
     1981 caggcaccat cgctccgcct cggcctgaac ctgccctaca cagaaggcca gatggacggc
     2041 gccacgccgc gctggacgga catccgggcg atggctgtgg ccgcggaggt ggtcgggttc
     2101 gacgccgtct ggatctccga ccatgtcggc ttcggggacc ccgcgagccc ggagcccggc
     2161 cgctggagcg gcgcctggga ctgctggacg ctgctcgcgg ccctggccgc ggtcaccacc
     2221 cgagtccatc tcgggacgta cgtcctgtgc accgcgttcc gcaacccggc gctcctcgcg
     2281 aagatggccg agacgctcga tgaggtcagt ggcggacggg tcatcctggg cctcggggcg
     2341 ggctggaacg agatcgagtt ctcctcctac ggatacccat acgcggaacg gttcgggcgc
     2401 ttcgaggatt cgctcaggat catcgccgcg atgctccgga ccggccgggc gacctacaac
     2461 gggcgctggg agcgggcggc cgatgcccgg atcgagcccc gtggcccgcg ggccgcgggg
     2521 ttgccgatca tggtcggcgc gggtggaccg cggatgctcc gcctgacggc cgagctcgcc
     2581 gaccgctgga acggcgggat gggcagcgtg gccggcacga tcgagacact ggccgcgctg
     2641 aatgaggcgt gccgagccgt cggccgggac ccggcttcga tcgagcggtc ggtcgaggtt
     2701 ctcgtccgac ccgggccggc gccggccgat gagcagggcg aaccgaacga gctccgcggc
     2761 tcgcccatcg aactcgctgc gtcgctcctc gcctaccggg ctctcggggt tggcgagctc
     2821 caggtccagc ttcgcccgaa cacgttggcc gcgatcgacg ccttcgcacc ggtcatcgcc
     2881 gcgatccgcc gcgggtgact gccatctgcc acgatccggg ctgacggatc acccggcccg
     2941 cggggccgaa tggccctgtc gcgggttgcc ctcgatcgcc atcgtccgat gcgtgagccg
     3001 cggtgacggt cgcgccggag gacccatcca ggatcgggga ccgctgtcga taccctgccg
     3061 gagtattgac aagggggttg tcaaatccgc cccgatcgag taggattcgt ccatcgctgg
     3121 cgtgtcaatg cttgacacgc cggtccgtgt gtccggctcc tggcggaagc ggatgcaccc
     3181 cgggccgcct cggcggatgc gcggtccggt tcgccagcaa gcatcccgca tcgtcctctc
     3241 cgcgcgactc gcctcgcgca tcgacaattc ctggagcgac cttgaccacc ttccttgacc
     3301 agacccccga cctcgatctc gacgcccgtc ccggcgcgac gccggctctc gacgagctgg
     3361 cgccaacccg cgaagacgtt gccgccgtcg tcgaacacga tccgaccctg accctcgtcg
     3421 cgcccgtcaa gaagacggcc gtggcaaagg cggcccgcga cgacgagccc gagatcgccg
     3481 agcatgacga cctgctcgac atcgaagact cgacccggct ttacctgcgg gagatcgccc
     3541 gcgtccccct cctcacggcc gaagaggagg tcatgctggc caagacgatg gagctcggcc
     3601 tccggatcac gaaggatccg gggctcgccg tcctcgatct tcatgtctgg accgcgaacg
     3661 ccagcgagcc caccgcgcgg gccaagcacc cgcgctacgc gatgaagttc gccgaccttg
     3721 tggcgcggat cgtgcgatcg gcgatcgaag cggacgaggc cagcgaattc ctcgtcacgg
     3781 cgccgcgctt cggcctgacc gaatcgatcg agggtgcggt cggcgaggcc gccgagctgc
     3841 tcgagcgggc tcgcaacatg cgatccgtct acaacgagcg cctcgatgcc gagacgttcc
     3901 tgacgaccct tgcctggatt catggggcag ccagtcgccc ggtggtccgc gagaaccccg
     3961 ccatgatcgc gatgcggacg tggacgcgtg acgccgtcgg cctgccggcc gttcggcgct
     4021 ggctggatgg cggccacgac acggacgcga tcgagaccat ggcgccgacc ctcgtcgcca
     4081 ccggcctcac cgcccgcgag cacctgacct cggccaacct ccggctcgtc gtcgccaacg
     4141 ccaagaagta catcaaccga ggcatgagcc tgctcgacct cgtccaggag ggcaatgccg
     4201 ggctgatgcg tggcgtcgac aagttcgagt gggagcgcgg cttcaagttc tcgacctatg
     4261 cgacgtggtg gatccggcag gcgatccagc gcggcctcgc ggaccagtcc cggacgatcc
     4321 ggatcccggt ccacatggtc gagacgatga accgcgtcac gcgaacgtcg cgcgagctca
     4381 ccgtcgagct cgggcgggag ccgacgaatg ccgagatcgc cgcgctcctc tccgaggatc
     4441 cgaagaccgc gatcacgacc gagaaggtcg aagaggtccg aagctatggc cgcaatccgg
     4501 tctcgctcga gacgccgatc ggcgacgaaa gcgacaccga gctcggcagc ctgatcgagg
     4561 acccggacgc gatctcgccg ctcgaggccg tgtcggacac gatgctccgc gagcaggtcg
     4621 cccgtgtcct cgacagcctc gagggtcgcg aggagcgggt catccgcctc cggttcggcc
     4681 tcgacgacgg ccggccgcgg acactcgagg aagtcggcca cgaattcggg ctgacccgcg
     4741 agcggatccg ccagatcgaa gcccacgcgc tgcgcaagct gcgccatccg tcgcgcagcc
     4801 gcaagctccg cgactacgtg accgactgag ggctgtcgcg cctggacgac gctggcggcg
     4861 ttgccgcctg cggtcctcgc tcacgaccat ccgaggtcgc tcgctgtggt cctcggcggc
     4921 gccttgccag cctcgcccat ccgcgacgaa cgtagttggg ggttcgggtc aggtcgccgg
     4981 atcggccggg cgcggcgccg caaggtccgc gacgacctcg acattcggca gcgtccggag
     5041 gatcgccggc ggggcgatca ccttccagct ccggctcccc ccgccgagga cgatccgctc
     5101 gcgatccatg accgcgccgt cgacccacag cggcaggtcg ttcggcagcc cgaacgcggt
     5161 cacgccgccg atctccatgc ctgtcaggat ccgcgtctcg tcgggcgagg cgaacgaggc
     5221 cttgcgggtc cggagtcgct gtttcacgac gcgattgaca tcgagtcgat gggtcgcgag
     5281 gacgacacag gcggcataga ccggcggatc cgacttgccg atcacgacaa tcgtattggc
     5341 cgagtcggcg gggtcgaagc cgtaagcggc gcagaacgcg gcggtgtcgg cgagggccgg
     5401 gtcgcaggcg aacagctcgt acgggccgtg gccggcgagc gcccggtcca gggactcgcg
     5461 agcaggatcg ctgtcggatc cggcggacgt cgtcatggcc gaacggtagc caatcccgcg
     5521 gatccgcgtg acatccgcgc cgcgcgctac gcgaccccga tgacccggat ggagcccacg
     5581 ccgccctgga ggtccaggtc gacgcggttg gcggcggtcg catagtcggg cgactcgtag
     5641 ccggtcgcgg ttcgcgggaa gcgtgactgg tcgacctggc tgctgccgat cgccatgcgg
     5701 ctccggatcc gggcggccac ctcggcggga acctcgatga tcagtgccgc cgcaccggtc
     5761 tccgcccgga ccgtgctggc cccggcggcc cgtggcaggc gaatgcgggt ctcgctcgcc
     5821 ccggtatgga gctcgaggtt ccggagcttc aggtcggtca ggtcgaggcg cgctcgcgcg
     5881 gcgcccgtgt cgagtcgaag gtcgagcggg acgcccgtcg cgaggccgac cgaccagtcc
     5941 gagcgtccgt cgagccacgg caggccgtag gtcgtgtcct gttcgagatc gacccgtccg
     6001 tgaccacgat cccggcgcac gacgccgccg ctgaagtgac cgtcgacgag attgccggcg
     6061 gcggcggctc cgaccgcgag ctcgcccgcc ccgaaccgga tcctgactgc cgcgtcggcg
     6121 acgccggcca gcgggatcac gagctcctcc atcggcgatc cgccgaaggg caggatcgcg
     6181 ccgatgacga accacacgcc gaggccgacg aggacccacg gccagccgtc ggagatcagg
     6241 tcgaccggtc cccggccgag attgccggtc gtgctcatca ggagaacgac gccgagcgcg
     6301 atccacagga ccggccacgt gaaggaccag atccccgcga tcccccgcgg gcggccgaag
     6361 gcgcgtgaca cgaagctcgc gacaccgccg agaatcagga gcattggcca cgcttcgccc
     6421 cagtcgagcg cgagagcctg acggacgagg aagacaaggc cgagtccgat cagccacgtc
     6481 gcggcgatga cggacccacg gtggatcgtc atgacctggt gctcccgccg ttcgcgtcag
     6541 ccggcttcga aggatccgat cgaggacgaa ccgcgaggac gagcacgagg acgccgacgc
     6601 cgacgaggac cagcggccag aaccagtccc agtcgatctc cgggaggtac tggcggacga
     6661 gggaccacgc gccgaccagg atgaacagga cgccgaccac gaccgcaccg gaccgtccgt
     6721 cgtagccgcg gctcgcgcgc cgcgccgccc gggcctcatg gcgggcggcc cgtcgcatgt
     6781 cgcgggcctc gaaccgtgac gcctgccaat ccgtcgccga cggtccggag ctcgccggga
     6841 tcgttgcggc ggccgaggcg ggctgcgcgg gcgacaggcc ggccgcgtcg gtcaccgacg
     6901 cagtctcggc cgtcggcgca ggcgcgcccc ctgccggagc gctgctcggc gatccggtcg
     6961 gggcgtccca ggccggcgcc cggctgcccc aggaacggct gccgtcctct tcgggaacga
     7021 cgatcgccat gacgatgtag acgaccaggg cgatcccgcc ggtcagcggg accagcaggg
     7081 cccagatgat gcgaacgagc gatggatccg cgtcccacag gtcggcgagg ccgccggcga
     7141 cccccgcgag catccggtcg tcgcgcgctc gatagagatg ggtgttcatc gacatcctcc
     7201 cacgggattc aggctgaagc tcgccgcgcc accgtcgatc gacagggtca cgatcggtgc
     7261 gccggcggct gccgggcggg tccacgtgtc gcccgagcga ctgaggccgc gggccgccag
     7321 gttatggacg aacgtgagct ggttggttac ctcgagccgc aggccgacgc cgtcgggaac
     7381 acacagatcg aaggcgcccg cgttcaccga gacgtcaccg ccgagaacgc tcgaaccggc
     7441 gtccagccgg atccgcccgg cgttcatcga caggtcgacg acgtcgacgg ccccatgcgt
     7501 cgcgtcgatc cgcaggtcgc cggcgttcat gtccacgtcg aggctggcga cgtgaccgtc
     7561 gggaaactga agcgtcgcgg tcgcggcatt ggcggtgatc gacagcaccc cgaccgcgct
     7621 cggcaggctg atcgtccagt cgttgcgccg ggcgctgccg cctccggaca cgcgaaggtc
     7681 caggcggctc gatgaggagt cgacgatcgg cgccgggccc cggtaggctg cgtcgagcgc
     7741 ccagttcgcg cccgtcgaag tcgcgaccgt gagcgatccg caggtgaggt cgaggcggac
     7801 ggccgctgat gggccgtcga acgatccgga ccgctggagc tgctggtcgg tcgggccgcc
     7861 gctgaccgcg caatccgaca gcgcgccgat ccacaggtgc ccgctggcga tggccgcccc
     7921 gccgaccgac cagcagctcc agcggtccgg atcgttcgac ggcccatcag cggccgtccg
     7981 cctcgacc
//
LOCUS       DATAIW010000301         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_168405, whole genome shotgun sequence.
ACCESSION   DATAIW010000301 DATAIW010000000
VERSION     DATAIW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168405"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..523
                     /locus_tag="VHM48_09350"
     CDS             218..523
                     /locus_tag="VHM48_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2755657.1"
                     /translation="MLSVKVSTKHQIVVPSEARARLGIEAGDRLDVRITDQAIVISKR
                     PSRASDRMRGIAAGRGWYEPDPVTYVRNLRDEWEERILEREARLGRSDVDATSADRD"
     gene            492..935
                     /locus_tag="VHM48_09355"
     CDS             492..935
                     /locus_tag="VHM48_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2755658.1"
                     /translation="MLTRHQRIAIDSNVLIYLTEGDVERADAAAAIVDSVAEGRTEGT
                     LSSAGLAEVLVGPARSGDGAGFERLAATIRDLGFGVTMLDATTAEDAAWIRGQTGMSL
                     PDSIHVACARSSGASAFVTNDRGIASRAGLEVLYLDDLIANQPPA"
     gene            1167..2363
                     /locus_tag="VHM48_09360"
     CDS             1167..2363
                     /locus_tag="VHM48_09360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2755659.1"
                     /translation="MDLTLTAAQLDLRARARAYVVDVLQPIEVEFERAGGRLPPASRD
                     QLKRAAIDARLHGGSLPPEIGGQGWSVLEQVIVHEQLGQATGGLWSFIPGAYNALIHA
                     DADQRRHYLDPSLRGERFGSYAITEPNAGSDARTLEATAIRDAATGDYILNGEKWFVT
                     GPPDETDFMIFHCLVGGGAASPAVVEPRPTLFLVDYDTPGLTMKHDPDYTHTFADRHP
                     QFVLEDVRVPASAILGGIGNADELTNEWFVEERIHIGARCAGAMERLLGLAVDWATNR
                     VQFGQRIYDFQGVSFPLADSAADASAARLLTREAAALADAGADPKVVHAKASLAKLFA
                     SEAAWRCADRVVQVFGGRGYMREFPAERYLRELRVDRIWEGTSEIQRVIVARALEKRG
                     VGRVVG"
     gene            2360..>3936
                     /locus_tag="VHM48_09365"
     CDS             2360..>3936
                     /locus_tag="VHM48_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2755660.1"
                     /translation="MTEASLLATGTGPVSPHPANPPPIDLRPLFAPRSIAVVGASARG
                     GIAQTVRNNLRVLESGTRCHFVNPRYDELDGQPCYPSIGGLPEVPDIVVVAVNPLRAA
                     PVVAAAAAAGVPAVVIPGGGVVEGGQAAAQMQRDVRDLAIRHGVALLGPNCMGLVDLT
                     TNSATYIGDVNPWLPRGGVAGIAQSGSVTDAFIHAGNRIGFSRIVGCGSEVVLDVCDF
                     LAHAIDDDQTEAVILFVEGFKRPERFLALADRALQVGKPIMAVKVGRSRQAQAAAVAH
                     SGSLAGEARATDAALAAAGVIVCEDLDRLLETAELVAGCRRMGRRVRRGRTGVVTVST
                     GEASLIADLAPRTGIDLPEVPPRARAALLADLPTLGYIGNPLDPWGATDPPTAYAAAF
                     EAFAASGAYDVLALVHDFPYRSLPSEVETALEVAAPLLAATRHRPEILPIFVSLTSGE
                     PTPEIVDAMQVGGGVPVLRGAVEAFTAIAAVGRWEAHHDRRRAGRPARADWPRLAADR
                     TLLGHDPAFVGLIGLEG"
BASE COUNT          547 a         1416 c         1382 g          591 t
ORIGIN      
        1 gatccgctgg ctgatccgga acacggagca gaagccgggt ggcggcgccg tccccgacca
       61 cgacacggcg ctcgcgctgc tgcgccagcg gttcgcccgc ggcgagatcg acgcgacgga
      121 gttcgaggag cggaagcgca ccctgggcgg ttgattcggt cccacagccc cccacgctgg
      181 ttgacggccg ctccccatct ccttagcatg acagatcatg ctaagcgtca aggtcagcac
      241 caagcaccag atcgtggtcc ccagcgaagc gcgcgcccgg ctgggcatcg aggcaggcga
      301 ccgactggat gtccggatca cggaccaggc gatcgtcatc agcaagcgtc cgtccagggc
      361 ttctgaccgc atgcgcggta tcgccgcagg tcgcggctgg tatgagcccg atccggtgac
      421 atatgtgcgc aaccttcgcg acgagtggga ggagcgaatc cttgagcgtg aagctcgcct
      481 tggtcgaagc gatgttgacg cgacatcagc ggatcgcgat tgacagcaac gtcctgatct
      541 acctcaccga aggagatgtc gaacgagccg acgcggcggc ggccatcgtc gactcggtcg
      601 ccgagggtcg gaccgaaggc accctttcga gcgcgggact ggccgaggtt ctcgtcgggc
      661 ctgcgcgttc cggcgatggc gccggattcg aacgtctcgc cgccacgatc cgtgacctgg
      721 gtttcggagt caccatgctc gatgcaacga ctgccgagga cgccgcgtgg atcaggggtc
      781 agaccggcat gagcctgccc gattcgatcc atgtcgcttg cgcgcgttcg tccggtgcct
      841 ccgccttcgt gacgaatgac cgcgggatcg cctcccgagc cggtctcgag gtgctgtatc
      901 tcgatgatct gatcgcgaac cagccgcccg cctgacggcg ggtcgagtcc tcgatgtcga
      961 tcagatccag atgttgctcg gctcgatcac ggcggtgagg gacggcattc gagctcgagc
     1021 cacaactcga tgccgcgttc gcgggcgttg tcggttcgcc gccatcggtt ccgcgtccgt
     1081 ctggtaaggc tgccgactca cgtgcgaccg tcgcacgacc cgggacgcgc ggcgcggccg
     1141 gcacccgggc cggtcatcct cgggcggtgg acctgaccct gaccgccgcc cagctcgatc
     1201 tccgggcgcg cgcccgcgcc tatgtcgtgg acgtgctcca gccgatcgag gtcgaattcg
     1261 aacgcgccgg tgggcggctc ccgcccgcca gccgcgatca gctgaagcgc gcggcgatcg
     1321 acgcccgcct gcatggcgga tcgctgccgc ccgagatcgg cggccagggc tggtcggtgc
     1381 tcgagcaggt gatcgtccat gagcagctcg gccaggcgac gggcgggctg tggtcgttca
     1441 tcccgggcgc ctacaacgcg ctcatccacg ccgacgccga ccagcgccgg cactatctcg
     1501 atccgagcct gcgcggcgag cgcttcggga gctacgcgat caccgagccg aacgcgggct
     1561 cggatgcccg gacgctcgag gcgaccgcga tccgcgacgc cgcgaccggc gactacatcc
     1621 tcaacggcga gaagtggttc gtcaccggac ctcccgacga gaccgacttc atgatcttcc
     1681 actgcctcgt cggaggcggc gccgcgagcc cggccgtcgt cgagccccgg ccgaccctct
     1741 tcctcgtcga ctacgacacg cccggcctga cgatgaagca cgaccccgac tacacccata
     1801 cgtttgccga ccgccatccg cagttcgtcc tcgaggacgt ccgggtcccg gcgtcggcga
     1861 tcctcggcgg gatcggcaat gccgacgagc tgacgaacga gtggttcgtc gaggagcgga
     1921 tccacatcgg ggcccgctgc gccggggcga tggagcgcct gctggggctg gcggtcgact
     1981 gggccacgaa ccgggtccag ttcggtcagc ggatctacga cttccagggc gtctcgttcc
     2041 cgctcgccga ctcggcggcg gacgcatcgg ctgcccgcct cctcacccgg gaagcagccg
     2101 ccctcgccga cgccggcgcc gacccgaagg tcgtccacgc caaggcgtcg ctcgccaagc
     2161 tcttcgcgtc ggaggccgcg tggcgctgcg cagaccgcgt cgtccaggtc ttcggcgggc
     2221 gcggctacat gcgggaattc ccggccgagc gctacctgcg cgagctccgg gtcgaccgga
     2281 tctgggaagg cacctcggag atccagcggg tgatcgtcgc ccgggccctc gagaagcgcg
     2341 gcgtcggccg ggtcgtcggg tgaccgaggc gtcgctgctc gcgaccggca cagggccggt
     2401 gtcaccccac cccgcgaacc cgccgccgat cgatctccgg ccgctcttcg ccccgcgctc
     2461 gatcgccgtc gtcggtgcct cggcccgcgg tgggatcgcc cagacggtcc gcaacaacct
     2521 ccgggtcctc gagagcggca ctcggtgcca tttcgtcaac ccgcgctacg acgagctgga
     2581 cggccagccg tgctacccgt cgatcggcgg tctgcccgag gttcccgaca tcgtcgtcgt
     2641 cgcggtgaac ccgctccggg cggcaccggt cgtcgccgct gccgcggcgg ccggcgtgcc
     2701 cgcggtcgtc atcccgggcg gtggcgtcgt cgagggcggg caggccgcgg cgcagatgca
     2761 gcgcgacgtg cgcgatctcg cgatccgcca tggggtcgcg ttgctcggcc cgaactgcat
     2821 ggggctcgtc gacctgacga cgaacagcgc gacgtacatc ggggacgtca acccgtggct
     2881 gccccgcggt ggcgtggccg ggatcgccca gtcgggttcg gtgaccgacg cattcatcca
     2941 tgccggcaac cggatcgggt tttcacggat cgtcggctgc gggagcgagg tcgtcctcga
     3001 cgtctgcgac ttcctcgccc acgcgatcga cgacgaccag accgaggccg tcatcctctt
     3061 cgtcgagggc ttcaaacgac ccgagcgctt ccttgccctc gccgaccggg cgctccaggt
     3121 cggcaagccg atcatggccg tcaaggtcgg ccggagccgc caggcgcagg cagcggcggt
     3181 cgcccattcg gggtcgctgg cgggcgaggc ccgggcgacc gatgccgccc tggcggccgc
     3241 gggggtgatc gtctgcgagg acctcgaccg gttgctcgag accgccgagc tggtcgccgg
     3301 ctgccgacgg atgggccggc gggtgcggcg ggggaggacc ggcgtcgtga cggtgtcgac
     3361 cggcgaggcg tcactgatcg cggacctcgc gccgcggacc gggatcgacc tgccggaggt
     3421 gccgccacgt gcacgtgccg cgctgctcgc cgaccttccg accctcggct acatcggcaa
     3481 tccgctcgac ccctggggcg cgaccgatcc gccgacggcc tacgcggcgg cgttcgaggc
     3541 gttcgccgcc tccggcgcct atgacgtcct cgccctcgtc cacgacttcc cgtatcgctc
     3601 gctgccgtcg gaggtcgaga cggcgctcga ggtcgccgcg ccgctcctgg ccgcgacccg
     3661 tcaccgcccg gagatcctgc cgatcttcgt ctcgctgacg tcgggcgagc cgacgccgga
     3721 gatcgtcgac gcgatgcagg tcggcggcgg cgtgcctgtg ctgcgggggg cggtggaggc
     3781 cttcacggcg atcgccgcgg tcggacgctg ggaggcccac cacgaccgcc gccgcgcggg
     3841 tcgaccggcg cgcgccgact ggccacggct ggccgccgac cgaacgctgc tgggacacga
     3901 tccggcgttc gtcggactga tcggcctgga ggggat
//
LOCUS       DATAIW010000302        11673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_49037, whole genome shotgun sequence.
ACCESSION   DATAIW010000302 DATAIW010000000
VERSION     DATAIW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49037"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHM48_09370"
     CDS             <1..230
                     /locus_tag="VHM48_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755661.1"
                     /translation="IAVYDEVGGILVAGDALRTNTGSLAGSDPQFTADPTAAAASVIK
                     LGRLRFETLLVGHGEPILTGASTQVAALGGG"
     gene            249..1928
                     /gene="pgm"
                     /locus_tag="VHM48_09375"
     CDS             249..1928
                     /gene="pgm"
                     /locus_tag="VHM48_09375"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX2755662.1"
                     /translation="MANSPRAGQPADPASLVDIPALLRAYDEITPDAADPTQRVAFGT
                     SGHRGSAFRGGFNEAHILAISEAICRYRELHGYDGPLFIGRDTHALSEPATRTALEVL
                     VARGVDVRIDSRDGYTPTPAVSHAIIVANRGRAVPASDSPAAAGRGLADGIVVTPSHN
                     PPDDGGFKYNPPNGGPADTDVTGWIQAEANRLLEARGNDGVAGIRRVPLEQARATGRG
                     HDFLATYVDDLSSVVDIEAIRASGLRIGVDPMGGASVAYWPAIAERYGLELTVVNARV
                     DPTFGFMTYDWDGKIRMDPSSPSAMAGLIALKDRFDIALGNDADADRHGVVTPAAGLL
                     NPNHFLSAAIAFLYGGARTWGADVGVGKTLVSSSMIDRVVADLGRRLVEVPVGFKWFV
                     AGLVDGSLGFGGEESAGASFLRRDGTVWTTDKDGIIADLLACELTARSGRDPGVAYHE
                     LTERFGSPAYRRIDAPASPEQKAALGRLAPSDVTATTLGGDPITAILTRAPGNDAPIG
                     GLKVTTANGWFAARPSGTENVAKVYAESFRGEEHLAKLLEEAQTLVASVTG"
     gene            2144..3907
                     /locus_tag="VHM48_09380"
     CDS             2144..3907
                     /locus_tag="VHM48_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2755663.1"
                     /translation="MKANARQRRNASATSDETSEAARLATSVGQVPADRLRTIFDGMF
                     DGVWLVAGDGRTTYANAAMATLLGTSPEEMRGHALSDYLDPEFWQAAAGFLKRQRIYT
                     AERMELRFRRADGEDLFGIVAGSPIATNDGIYVGTMLNVSDVTAKRGIDAQMIQNQRL
                     EAIGQFAGGIAHDFNNLLTAIQGFTQIALASLPEADPIRDDLTQVIASTERATAITRK
                     LLAFTRRQVLMPLDIDPAVVIAALLPILAPLLGEVIIDLDVAANHAWVRVDPTQLEQI
                     ILNLAVNARDAMPDGGTVRISVHNLESADPDRPDPDLTAGPFVRISVSDTGTGMDEAT
                     KARIFDPFYTTKAEGKGTGFGLSTVFGIVAQSGGQVQVETAVGAGSTFHVDLPRVVAT
                     AGMVQPVPIAASTRGTGAVLLAEDDPAVREFTRRSLEAAGYTVFAVAGAEDAIHASEH
                     WSSPIDVLVTDIQMPGMHGHELAVRIRGARPGIGIVLISGYTQDVVGKGTELGMVEFL
                     SKPFTIDALTTAVWRAADVARRRAAAAPRPGRPAAGPVRKPARAVPIRRSGETKSSRA
                     PITVPTKRRSGVAPSTRSRSG"
     gene            4070..4327
                     /locus_tag="VHM48_09385"
     CDS             4070..4327
                     /locus_tag="VHM48_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755664.1"
                     /translation="MATTIDLRVRLIPWDDPTFVHAFETAREQVRAEGVTINGPDACA
                     RVEALLRDAGFPNVRVDCVRTPDEAMAHIAHWTVRRDGPFD"
     gene            4413..4991
                     /locus_tag="VHM48_09390"
     CDS             4413..4991
                     /locus_tag="VHM48_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755665.1"
                     /translation="MIRRLGPGRASLLVIGLLALVAAIAIPALAASPSPSPGANPGKG
                     PKASEAPAVAVTLHGVVGAMKDADGDTTYSLSVDGTSVQLDAGPPWFYGDNHPLAAFV
                     GKSVTITGSRRGAEVDVETVDGVMLREPGKPPWAGGWKAVGSAHPGWSQAKADRSQQK
                     QADKQAGGVAGCWPPGHCKQNEPESSANPDLR"
     gene            complement(5004..6071)
                     /locus_tag="VHM48_09395"
     CDS             complement(5004..6071)
                     /locus_tag="VHM48_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2755666.1"
                     /translation="MTFQWVGLLVLLAIVPLVVAVYAWSLRRRRPSAARYSSLALIRD
                     AAPGSSRMRRHLPFALIAAAIVALVVALGRPAVVVGVPTNQTTIILTMDVSGSMCSTD
                     IAPTRLEAAEEAAISFVTSQQARTEIGIVAFSGFAAVVQPPTSDQTVLVDAIRSLTTG
                     RRTAIGSGILAAIDAIAEVDPTIQRSVVAGRPGSEPPPVAAGAYAPDIIVLLTDGANN
                     AGPAPVDAAQQALDRGLRVYTIGFGSVDGGSLDPICRQQFIGNEPGGGFGGGGFGGGG
                     GGRFPRGIDEPTLRQVADLTGGSYYPADSAAELERVFAELPTNLITKREALEISVGFV
                     GLGALLAAFGLLLGRAWRPLP"
     gene            complement(6088..7095)
                     /locus_tag="VHM48_09400"
     CDS             complement(6088..7095)
                     /locus_tag="VHM48_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2755667.1"
                     /translation="MTFLWPVVLIGVALVPAWFVATWRIDARRRRRIAGLGGLGRETG
                     GSARRGSSAARVATLLVTSAFVVLAVALARPQATVSLPRVEGTLVLTFDVSASMAADD
                     VAPTRMEAAKAAAKAIVERQPPGVVVGVVAFSDAGLAVQTPTSDQAAVLAAIDRLAPS
                     RGTSLGQGILASLGTIAQAESDTPANYYSNRSAEPTATPAPVAPGSHDAAAIVLFSDG
                     ENNERPDPMGAAQAAADRGIRIVTVGVGTAAGSTLDLDGFRVQSALDEAALRAVADLT
                     AGRYEAADALDPATVYDQLSEHLVVRGEALELTAVFAAVGLLLLLGGTIVSLARSGRL
                     P"
     gene            complement(7092..7997)
                     /locus_tag="VHM48_09405"
     CDS             complement(7092..7997)
                     /locus_tag="VHM48_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2755668.1"
                     /translation="MAFASAPERILQRLDWQVVRRLDGLLQGDYRSLFHGTGLDFAAL
                     REYQFGDDVRAIDWNVTARMATPYVREYHEERDITAWFLLDLSPSVDFGTVAAEREKR
                     TVLVDFAATMARLLTRRGNRVGTMVFAGTGTHIIPAGSGRNHVLRMIEDLVEQPRLAA
                     APITDLGPLLEAAHRRIRRRSLIVLVSDFIGVPGWERSLELLNRRHELLAVRLIDPRE
                     VELPDVGPMVMEDAETGEQLYVDTSDATFRRRFRAAADAREAALAAAFRRARVEATTL
                     STEDDLVRAIVRMATSRRRRRRRPT"
     gene            complement(7975..8985)
                     /locus_tag="VHM48_09410"
     CDS             complement(7975..8985)
                     /locus_tag="VHM48_09410"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2755669.1"
                     /translation="MDSVSNGRPDAPMERLLYEVKKVIVGQDHLLERMAVALLARGHL
                     LVEGVPGLAKTMAIKALAQSIGGEFKRIQFTPDLVPADLVGTRIYNQKTGEFTTSLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQERQVTIGHDTHPVPNPFLVLATQNPIETEGT
                     YALPEAQVDRFMLKVLVGYPSPTEEFAIVERMTGRLDRISQVLSTDELVRLQETVDGV
                     YVDPALVEYAVRLVSATRAPQAHGMPELQRYLTWGASPRASINLILTGRALAYVRGRE
                     YVLPQDVRDMAFDVLRHRLVLSFEALSDDISPDRILSSIVGRVPMPVVPMQEHRGVRV
                     GA"
     gene            complement(9011..9883)
                     /locus_tag="VHM48_09415"
     CDS             complement(9011..9883)
                     /locus_tag="VHM48_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2755670.1"
                     /translation="MIEQPDTTRRPEPGSPPGRWQRVRARARKTRSAVRRGAPFVAGV
                     LATFIAIAAYGALRPSPAPLSIRDVNRAVASALASQTPGPARSELVYAAARPSLVLIE
                     TDGTDAKGAAEGGLGSGVVVNAVGSVLTALHVVADATAIRLTFADGSKSTATIASQDP
                     ATDIAVLTPKAPPATLVPATLGNPGAMHIGSEAYIVGNPFGLYGSLSSGVVSGLDRSF
                     QEPAGTRVYHGLIQVDAAVNPGNSGGPLLDRDGRVVAIVTALINPTKQDVFIGIGLAV
                     PIDVAGGGAGLPPY"
     gene            10154..11434
                     /locus_tag="VHM48_09420"
     CDS             10154..11434
                     /locus_tag="VHM48_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2755671.1"
                     /translation="MSHLDDYLARTPRSRALFERATASLPGGSTRTTVYSAPYPPYAA
                     AAAGLSFTDVDGNVYRDFLGNYTSLILGHAHPAVVAAVEAQVRRGSAFAAPTETEVEL
                     AEELRRRVPSVEHLRFTSSGTEATMFAIRAARAFTGRPLIAKFDHSYHGTHDLVMAGT
                     PGVPEAIGGLIVELPWGDPDGISALLRGREGELAAVIVEPVQGAGGVRAPEPGFLEWL
                     RALCDRIGALLIFDEIISFRIAPGGAQEVYGIRPDLTTLGKIIAGGYPLAAFGGRADV
                     MAIFDARRGRAVSHGGTFNGNPVAAAAGLATLRELTPDAYVRLGDLGRRLFDRLSVAI
                     AAGGLDARAVNVGSLFQVFRGGAGAGAAFAPGAGQPSELFLGLLLEGFYLAPRGMGAI
                     PTVATEQDVDDLADAITRVLGAIERVPATAGAGS"
BASE COUNT         1710 a         4141 c         4172 g         1650 t
ORIGIN      
        1 acatcgcggt ctacgacgag gtcggcggga tcctggtcgc cggtgacgcc ctccggacca
       61 acaccgggtc gctggcaggc tccgacccgc agttcaccgc ggatccgacc gcggcggcgg
      121 cttcagtgat caagctcggc aggcttcggt tcgagacgct gctcgtcggc cacggcgagc
      181 cgatcctgac cggcgcctcg acgcaggtcg ccgcactcgg cggcggctga ctcgggtaca
      241 ttcggcacgt ggccaactcc ccgcgcgccg gccagccggc cgatcccgcg agcctcgtcg
      301 acatcccggc gctcctgcgc gcctacgacg agatcacacc ggacgcggcg gacccgaccc
      361 agcgggtcgc gttcgggacg tccgggcatc gcggctcggc cttccgcggc gggttcaacg
      421 aggcccacat cctcgcgatc agcgaggcga tctgccgcta tcgcgagctg cacggctacg
      481 acggtccgct gttcatcggc cgcgacacgc acgccctgag cgagccggcg accaggacgg
      541 ccctcgaggt cctcgtcgct cgcggcgtcg acgtccgtat cgacagccgc gatgggtaca
      601 ccccgacccc ggctgtgtcg cacgcgatca tcgtcgccaa ccgcggacgc gccgtgccag
      661 cctcggactc cccggcggcc gccggacgcg gcctcgccga cgggatcgtc gtgaccccgt
      721 cgcacaaccc gcccgacgac ggcggcttca aatacaaccc accgaacggt ggcccggccg
      781 acaccgacgt gaccggctgg atccaggcgg aggcgaaccg gctgctcgag gcgaggggca
      841 acgatggcgt ggccgggatc cggcgcgttc ccctcgagca ggctcgggcc accggtcgcg
      901 gccacgactt cctcgcgacg tacgtcgacg acctctcgtc cgtcgtcgac atcgaggcga
      961 tccgggcctc cggcctgcgg atcggcgtcg acccgatggg cggcgcttcg gtggcctact
     1021 ggccggcgat cgccgagcgg tacggtctcg agctgacggt tgtcaacgcc cgggtcgacc
     1081 cgaccttcgg attcatgacc tacgactggg acggcaagat ccggatggac ccgtcgtcgc
     1141 cgtcggcgat ggccggcctg atcgcgctca aggaccgctt cgacatcgcc ctcggcaacg
     1201 acgccgacgc cgaccgccac ggagtggtca cgccggcggc cgggcttctc aacccgaacc
     1261 atttcctgag cgcggcgatc gcgttcctgt atggcggagc acggacctgg ggtgccgacg
     1321 tcggggtggg aaagacgctg gtctcgtcgt cgatgatcga tcgggtcgtc gccgacctcg
     1381 ggcgtcgcct ggtcgaggtt cccgtcgggt tcaagtggtt cgtcgccgga ctggtcgacg
     1441 gctcgctcgg gttcggcggc gaggagagcg caggcgcatc gttcctccgc cgcgacggga
     1501 ccgtctggac gaccgacaag gacgggatca tcgccgatct cctcgcctgc gagctgaccg
     1561 cgcggagcgg acgcgatccg ggtgtcgcct accacgagct gaccgaacgg ttcggttcgc
     1621 cggcctatcg tcggatcgac gcgcccgcat caccggagca gaaggcggcg ctcggtcgcc
     1681 tcgcgccgtc cgacgtgacg gcgacgactc tcggtggcga tccgatcacc gccatcctca
     1741 cccgggcccc cggcaacgac gccccgatcg ggggcctgaa ggtcaccact gccaacggct
     1801 ggttcgccgc gcggccctcc ggcacggaga acgtcgccaa ggtctacgcc gagagcttcc
     1861 ggggcgagga gcacctcgcg aagcttctgg aggaggccca gacgctcgtc gcgagcgtca
     1921 ccggctgagg ccgcgacggc ctccagccgg tcgccgcgag cgacggatca ccttcgggat
     1981 cggcccgccc cggatgaccg actcgtcatg acaggtcaag cgccgggacg cacactctgg
     2041 aaccgaggac ccggcgtttg ccgggtggtt cgaccacggc tcgaaggccc gtggacgcag
     2101 accgctcgat tgaagtcgtc cgggggacag gggacggtgg acgttgaagg cgaatgcaag
     2161 gcagcgccgg aacgcttccg cgacgtcgga tgagaccagc gaagctgcgc gactcgcgac
     2221 atccgtcggc caggtcccgg ccgatcgact gcgcacgatc ttcgatggga tgttcgacgg
     2281 tgtgtggctg gtggccggcg atggccggac gacctatgcg aacgcggcga tggccacgct
     2341 cctcgggacg tcgcccgagg agatgcgcgg ccacgccctg tccgactacc tcgacccgga
     2401 gttctggcag gcggcggccg gcttcctgaa gcgccagcgc atctacacgg ccgaacgcat
     2461 ggagctgcgc tttcggcgag ccgatggcga agatctgttc gggatcgtcg ccggcagtcc
     2521 gatcgcgacg aatgacggca tctatgtcgg gacgatgctc aatgtcagcg acgtgaccgc
     2581 caagcgtggc atcgacgccc agatgatcca gaaccagcgt ctcgaggcga tcggccagtt
     2641 cgccggcggc atcgcccacg acttcaacaa cctgctgacc gccatccagg gcttcaccca
     2701 gatcgcgctg gcgagtcttc cggaggccga tccgatccgg gacgacctga cccaggtgat
     2761 cgccagcacc gagcgggcca cggccatcac ccgcaagctc ctcgccttca cccgccgtca
     2821 ggtcctgatg ccgctcgata tcgacccggc cgtcgtcatc gcggccctgc tcccgatcct
     2881 cgcgcccctg ttgggcgagg tcatcatcga cctcgacgtg gccgcgaacc acgcctgggt
     2941 tcgggtcgac cccacccagc tcgagcagat catcctgaat ctcgccgtca acgcccgtga
     3001 cgcgatgccc gacggcggga cggtccggat ctcggtccac aacctcgagt cggcggatcc
     3061 ggatcggccg gatcccgatc tgacggctgg gcccttcgtc cggatcagcg tgtcggatac
     3121 gggaaccggc atggacgagg ccacgaaggc caggatcttc gatcccttct acacgacgaa
     3181 ggccgagggc aagggaaccg gattcggcct gtcgacggtc ttcgggatcg tcgcccagtc
     3241 cggtgggcag gtccaggtcg agaccgcggt cggcgcgggt tcgacgttcc acgtcgatct
     3301 ccctcgtgtg gtggccacgg ccgggatggt ccagcccgtt ccgatcgcag cgtcgacgcg
     3361 cggcaccggt gccgtcctgc tggccgagga cgatccggcc gttcgcgagt tcacccggcg
     3421 cagcctcgag gctgcgggat acaccgtctt cgccgttgcc ggcgcggagg acgccatcca
     3481 cgcgagcgag cactggagct caccgatcga cgtcctggtg accgacatcc agatgcctgg
     3541 catgcatggg cacgagttgg ccgtccgcat ccgcggagcc cgccccggca tcgggatcgt
     3601 cctcatcagc gggtacaccc aggatgtcgt cggcaagggg accgagctcg gcatggtcga
     3661 gtttctcagc aagccgttca ccatcgacgc cctcacgact gcagtctggc gggcggccga
     3721 tgtcgcccgg cggcgggcag cagccgcacc cagacccggc cgtccggccg ccgggccagt
     3781 tcggaagccc gctcgcgcgg tccccattcg tcgatccggc gagaccaagt cgagccgagc
     3841 tccgatcacc gtgccgacga agcggcgttc gggcgtcgcg ccgtcaacca gatcgcgatc
     3901 cggctgaacg acccccagga cgtccggacg atcgatccgc gcgggcgccg cccccggact
     3961 caggcggacg ccagcgccct ggattcggga cacccgagcg caagaacggg ccgttcggcc
     4021 gcagcgggtc ggggcggcgc cggcgcagac tcggtctgga ggtgacgcca tggcgaccac
     4081 catcgatctt cgggtgcggc tgatcccgtg ggatgacccc acgttcgtgc atgccttcga
     4141 aacggctcgc gagcaggttc gagcggaggg cgtgacgatc aacggcccgg atgcctgcgc
     4201 ccgggtcgag gcgctcctcc gcgatgccgg cttcccgaat gtccgggttg actgcgtgcg
     4261 cacacccgac gaggccatgg cccatatcgc acactggacg gtccgccgcg atggaccgtt
     4321 cgactgaccg cggcgaaacg ccggtgcatc gcagcaccgg tcgcgcgcca cgaggcatcg
     4381 atgtcgccaa acccgacgcg tacactcggc ccatgatccg tcgcctgggt ccgggcaggg
     4441 cgtccctcct cgtcatcggg ctcctcgcac tcgtcgccgc catcgccatc ccggccctgg
     4501 ccgcctcgcc gtccccgtcg cccggcgcga atccgggcaa gggtccgaaa gcctcggaag
     4561 cgcctgcggt cgcggtcacc ctgcacggcg tcgttggcgc gatgaaggac gccgacggcg
     4621 acacgaccta cagcctcagc gtcgacggga cgagcgtcca gctcgatgcg ggaccgccct
     4681 ggttctacgg cgacaaccat cccctcgccg cgttcgtggg caagtccgtg acgatcaccg
     4741 gcagccgacg cggcgctgag gtcgacgtcg agaccgtcga cggcgtcatg ctccgcgagc
     4801 ccggcaagcc gccctgggct ggaggctgga aggcggtcgg ctcggcccat cccggatggt
     4861 cgcaggccaa ggcggatcgc tcgcagcaga agcaggccga caagcaagcc ggcggcgtgg
     4921 ccggatgctg gccgcccggc cattgcaagc agaacgagcc tgaatcgtcg gcgaatcccg
     4981 acctgcgctg atccggaccg gtctcagggc aatgggcgcc aggcccggcc gagcaggagc
     5041 ccgaacgcgg cgagcagcgc cccgaggccg acgaacccga cgctgatctc gagtgcctcg
     5101 cgcttggtga tcaggttcgt cggcagctcc gcgaacacgc gctcgagctc cgccgcgctg
     5161 tcggccgggt aatagctgcc gccggtcagg tcggcgacct ggcggagcgt cggctcgtcg
     5221 atcccgcgcg ggaaccgccc gccgccgccg ccgccgaacc cgccgccgcc gaacccgcca
     5281 ccgggctcgt tgccgatgaa ctgctggcgg cagatcgggt ccagggagcc gccatcgacc
     5341 gacccgaagc cgatcgtgta gacccgcagg ccccgatcga gcgcctgctg cgccgcgtcg
     5401 accggcgcgg gcccggcgtt gttcgcgccg tcggtcaaca ggacgatgat gtccggcgcg
     5461 taagccccgg ccgcgacggg cggtggctcg ctgcccggtc gcccggcgac gacgctccgc
     5521 tggatcgtcg ggtcgacctc ggcgatggcg tcgatcgcgg caaggatgcc gctcccgatc
     5581 gccgtccggc ggccggtcgt caggcttcgg atggcgtcga cgaggaccgt ctggtcggac
     5641 gtcggtggct ggacgaccgc ggcgaacccg ctgaacgcga cgattccgat ctcggtccgc
     5701 gcctgctggc tggtgacgaa cgagatcgct gcttcctcgg cggcttcgag ccgggtcggg
     5761 gcaatgtccg tcgagcacat gctgcccgag acgtccatcg tcaggatgat cgtcgtctgg
     5821 ttcgtcggga cgccaacgac gacagccggc cggccgaggg cgacgacgag cgcgacgatc
     5881 gccgccgcga tgagcgcgaa cggcaggtgc cggcgcatcc gtgacgaccc cggcgccgca
     5941 tcgcggatca gggcgaggct cgaataccgc gccgcactcg gccgccgccg ccgcaggctc
     6001 caggcgtaga ccgcgacgac gagggggacg atcgcaagca gcacgagcag accgacccat
     6061 tggaacgtca tcgtcggtcc tcagtgctca tggcagccga cccgagcggg cgagcgacac
     6121 gatcgttccg ccgagcagca ggagcaggcc gaccgccgcg aacacggccg tgagctcgag
     6181 cgcctcgccg cggaccacga ggtgctccga cagctggtcg tagaccgtcg ccgggtcgag
     6241 ggcgtccgcc gcttcgtacc tcccggcggt cagatccgcg accgcgcgga gggccgcctc
     6301 gtcgagcgcg ctctggaccc tgaatccgtc gagatcgagg gtcgacccgg cagccgtgcc
     6361 gacgccgacg gtcacgatcc ggataccgcg gtcggcagcg gcctgcgccg cgcccatcgg
     6421 atcgggtcgc tcgttgttct cgccgtcgct gaacaggacg atcgctgccg cgtcgtgact
     6481 ccccggcgcg accggcgcgg gtgtggcggt cggctcggcg gaacgattgc tgtagtagtt
     6541 cgccggggtg tcgctctccg cctgggcgat cgtcccgagc gacgcgagga tcccctggcc
     6601 gagcgaagtc ccgcggctcg gcgcgaggcg atcgatcgcg gccagcacgg cagcctggtc
     6661 ggaggtcggg gtctggaccg cgagacccgc gtcgctgaac gcgaccacgc cgacgacgac
     6721 gccgggcggc tggcgctcga cgatcgcctt cgccgccgcc ttcgcggcct ccatccgcgt
     6781 cggcgccacg tcgtcggcgg ccatgctggc ggacacgtcg aacgtcagga cgagggtgcc
     6841 ctcgacccgc ggcagcgaga ccgtggcctg tggccgggcg agggccaccg cgaggaccac
     6901 gaaggcggac gtgacgagga gcgtcgcgac ccgggccgcg ctgctgcctc ggcgcgccga
     6961 gccgcccgtc tcccgtccca gcccgccgag cccggcgatc cgccgccgcc gtcgcgcgtc
     7021 gatccgccac gtcgcgacga accaggccgg gacgagcgcg acgccaatga gcacgaccgg
     7081 ccacaggaac gtcatgtcgg tcgccgccgc cggcggcgac gtgacgtcgc catccggacg
     7141 atcgcccgga ccaggtcatc ctcggtcgag agcgtcgtcg cctcgacccg cgcccgccgg
     7201 aacgcggccg cgagggcggc ctcacgggcg tccgcggccg cccggaagcg ccgccggaac
     7261 gtggcgtcgg acgtatcgac gtacagctgc tcgccggtct cggcgtcctc catgaccatc
     7321 ggcccgacgt cggggagctc gacctcgcgc gggtcgatga ggcgaaccgc gagcagctcg
     7381 tggcggcggt tcagcagctc gagcgagcgt tcccagcccg gcaccccgat gaagtccgag
     7441 acgagcacga tcagcgagcg ccgccggatc cggcgatgcg cggcctcgag gagcgggccg
     7501 aggtcggtga tcggcgccgc ggcgagacgc ggctgctcga cgaggtcttc aatcatccgc
     7561 aggacgtggt tccggccact gccggccggg atgatgtggg tcccggtccc ggcgaacacc
     7621 atcgtcccga cccggttgcc gcggcgggtg agcagccgag ccatcgtcgc ggcgaagtcg
     7681 acgaggacgg tccgcttctc gcgctccgcc gcgaccgtcc cgaagtccac cgacgggctg
     7741 aggtcgagca ggaaccaggc ggtgatgtcg cgctcctcgt ggtactcgcg gacgtagggc
     7801 gtggccatcc gggcggtcac gttccagtcg atcgcccgga cgtcgtcgcc gaactggtat
     7861 tcgcgcagcg ccgcgaagtc gagcccggtc ccgtggaaca ggctccggta gtcgccctgg
     7921 agcaggccgt ccaggcgccg gacgacctgc cagtcgagtc gctggaggat gcgctcaggc
     7981 gccgacgcga acgccacggt gttcctgcat gggtacgacg ggcatcggga cgcgaccgac
     8041 gatggacgac aggatccggt cgggggagat gtcgtccgac agggcctcga atgacagcac
     8101 gagacggtga cggaggacgt cgaaggccat gtcccggacg tcctggggca ggacgtattc
     8161 ccggccgcga acgtaagcga gggcccgacc ggtgaggatc aggttgatcg aggcccgggg
     8221 gcttgcgccc caggtgaggt agcgctggag ctcgggcatg ccgtgggcct ggggcgcccg
     8281 ggtggcgctc acgaggcgga ccgcgtattc cacgagggcc ggatcgacgt agacgccgtc
     8341 gaccgtttcc tggagccgga cgagctcgtc ggtcgagagc acctgggaga tccgatcgag
     8401 ccggccggtc atccgctcga cgatcgcgaa ctcctcggtc ggcgatggat agccgacgag
     8461 gaccttgagc atgaagcgat cgacctgggc ctcgggcagg gcgtaggtgc cctcggtctc
     8521 gatcgggttc tgggtggcga ggacgaggaa cgggttcggg accgggtggg tgtcgtggcc
     8581 gatcgtgacc tggcgctcct gcatcacctc gagcagcgcg ctctggacct tggccggggc
     8641 ccggttgatc tcgtcggcga ggagcaggtt cgtgaagacg ggtccgagcg aggtcgtgaa
     8701 ctcaccggtc ttctggttgt agatccgcgt cccgacgagg tcggccggaa cgagatccgg
     8761 ggtgaactgg atccgcttga actcgccacc gatcgactgg gccagtgcct tgatcgccat
     8821 cgtcttggcg agccccggca cgccctcgac gaggagatgg ccgcgggcga ggagggcgac
     8881 cgccatccgc tcgaggagat gatcctggcc gacgatgacc ttcttcacct cgtagagcaa
     8941 gcgctccatc ggagcgtccg ggcggccgtt cgagacggaa tccatcgacg tcacctcctc
     9001 caggttcact tcagtacggc ggcaggccgg cgccgccgcc ggcgacatcg atcggcacgg
     9061 cgaggccgat cccgatgaag acgtcctgct tggtcgggtt gatgagggcc gtcacgatgg
     9121 cgacgacgcg gccatcgcga tcgagcagcg gcccgcccga gtttcccggg ttgaccgcgg
     9181 cgtcgacctg gatcaggccg tggtagacgc gagtgccggc gggctcctgg aacgagcggt
     9241 cgaggcccga aacgaccccg ctgctgagcg agccgtacaa cccgaacggg ttgccgacga
     9301 tgtaggcctc gctgccgatg tgcatcgcgc ccgggttgcc gagcgtggcc gggacgagcg
     9361 tcgcgggcgg cgctttgggc gtgaggacgg cgatgtccgt cgccggatcc tggctggcga
     9421 tcgtcgccgt cgacttcgac ccgtcggcga aggtcagccg gatcgcggtc gcgtcggcga
     9481 cgacgtggag tgcggtcagg acggagccga cagcgttgac gacgacgccg ctgcccagcc
     9541 cgccctcggc cgcacccttg gcatcggtcc cgtcggtctc gatgaggacg agcgacgggc
     9601 gggcggcggc gtagacgagc tcggatcggg cagggcccgg cgtctgcgaa gcaagggcac
     9661 ttgcgactgc ccggttcacg tcgcggatcg acagcggcgc tgggctgggc cgcagcgcgc
     9721 cgtaggccgc gatggcgatg aacgtggcga ggacgccggc gacgaacggt gcgccacggc
     9781 ggacggcgga tcgtgtcttg cgggcccgcg ctcgcacgcg ttgccagcgt cccggcgggg
     9841 atccgggctc cgggcggcga gtcgtgtccg gctgctcgat catcgacagc ggagactccg
     9901 tgggtacgcg atgtgctgga cggtcgtggc cacgacggcg cctccgcggg agcgtcccgg
     9961 tcccgcctga gaagggcctg agaacggggc aaccaacgcc gcactgagcg ccgcgccctc
    10021 gaccgtgccc tgggtgccca gtctagacgt gccgccccga tgtgttcccg agcggggatg
    10081 ccgccctcat cggtatcgtt cgacggaagc gttcgcgcgg ttttccgcga cgggcgacac
    10141 acggaggcct tcgatgtcgc acctcgacga ctacctcgcc cggaccccgc gatcgcgtgc
    10201 cctcttcgag cgggcgaccg cctcgctgcc gggcggctcg acccggacga cggtctacag
    10261 cgcgccctat ccgccctacg ccgccgccgc cgcgggtctg tcgttcaccg atgtcgacgg
    10321 caacgtctac cgcgacttcc tcggcaacta cacctcgctg atcctcggcc atgcccatcc
    10381 ggcggtcgtc gcggcggtcg aggcccaggt ccggcgcggg tcggcgttcg ccgccccgac
    10441 cgagaccgag gtcgagctgg ccgaggagct ccggcggcgg gtgccctcgg tcgagcacct
    10501 gcggttcacc tcgtccggca ccgaggcaac gatgttcgcg atccgggccg cgcgagcctt
    10561 caccggccgg cccctcatcg cgaagttcga ccactcgtac cacggcaccc acgacctggt
    10621 gatggccggc acgccgggcg tgcccgaggc gatcggcggg ctgatcgtcg agctgccgtg
    10681 gggcgacccc gacgggatct ccgcgctgtt gcgcggcagg gaaggggagc tggccgcggt
    10741 gatcgtcgag ccggtccagg gcgcgggagg cgttcgtgcc ccggagcccg ggttcctcga
    10801 atggctgcgg gccctgtgcg accggatcgg cgcgctgctg atcttcgacg agatcatctc
    10861 gttccggatt gccccgggcg gggcccagga ggtgtacggc atccggccgg acctgacgac
    10921 cctcggcaag atcatcgccg gtggctatcc gctggcggcg ttcggcggtc gcgccgacgt
    10981 gatggcgatc ttcgacgccc ggcgcggccg ggcggtcagc catggtggga cgttcaacgg
    11041 caacccggtt gccgccgccg ccgggctcgc gacgttgcgc gagctgacac cggatgcgta
    11101 cgtgcgactc ggcgaccttg gccggcggct cttcgatcga ctgtcggtgg cgatcgccgc
    11161 cggcgggctc gacgcccggg ccgtcaacgt cgggtcgctg ttccaggtgt tccggggcgg
    11221 ggccggtgcc ggcgccgcgt tcgcgccggg cgccggccag ccgtcagagc tcttcctggg
    11281 gctgctgctc gagggcttct acctggcccc gcgcggcatg ggcgcgatcc cgacggtcgc
    11341 gaccgagcag gacgtcgacg acctggccga tgcgatcact cgggtcctcg gcgcgatcga
    11401 gcgggtcccc gcgacggcgg gtgccggcag ctgatgcacg gacgcgcgag cccgatcctg
    11461 cccatacgct cgtggggctg gcattagccg gcgcggcgag acggttcgcc ggctcggtcg
    11521 tgccgatatc tgggtataca cccattcttg atgcttggcg ggcccgccag acgagccgtt
    11581 ccgcacgcta accgacacgc tcgctggcgt ataggaaact cccggcgggc acgcgaccgc
    11641 gcatcgctca cgggacccgc gccgaccgac ggg
//
LOCUS       DATAIW010000303         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_276515, whole genome shotgun sequence.
ACCESSION   DATAIW010000303 DATAIW010000000
VERSION     DATAIW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276515"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VHM48_09425"
     CDS             <1..1258
                     /locus_tag="VHM48_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2755672.1"
                     /translation="RGIAVISDLVLNHTSDQNPWFTDSLAGTGHEDWYIWSATNPGYG
                     GPDGQQVWHPGGKRWYYGQFGAGLPDLNLRNPAVTAEMEAVARFWITTVGFDGFRLDA
                     IKHLIEDGQTQVNTPETHAWLQAFRTTLHDTAPNALLVGEVYDLAEAVAPYVPEDVDM
                     AFDFGLAQATIDSIRRGDAGPLNGAFAEDARLFLPDEGASFLTNHDQSRIASQLREDP
                     AALRLAAGLLFTDARTPFVYYGEEIGLTGEKPDEEIRTPLAWTGQAPAAGFTTGTPWE
                     SLAPGWETRNVAAEAADPTSLLSAYRDLIRLHDGHRALWAGSIEPLRASDSAVAAWLR
                     VGQGEQLAVVANLSEQPVTGATLDLDRGPLCGESTARVLYQGGVADPGGVALTPAAID
                     ADGGFRNWTPLTVLPARSITVISLGG"
     gene            1264..2739
                     /locus_tag="VHM48_09430"
     CDS             1264..2739
                     /locus_tag="VHM48_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX2755673.1"
                     /translation="MSVDVPAWAADAVFYQVFPDRFARSGRVPAPGPFEAWEAPPTHA
                     GYKGGDLYGVVERLDELADLGISGLYLNPIFTAASNHRYNASDYLTVDPLLGGTEALR
                     ALLDAAHGRGIRVLLDGVFNHAGRGFFPFQHVIEAGAVSPYRDWFYLDPDVLSGQRGI
                     DPYPTGDPEGHHLRAGYRSWWNFPSLPKLRVEHPPVREFLFGVAEHWLRFGIDGWRLD
                     VPAEIEDETFWPEFRQRVRAVRSDAYLVGEVWSESPEWLTGNRFDALMNYPLGLAILG
                     FASAGQLDQAAIDGQADYKRFLHPMDGPAFGDRLRHLFALHDPATTSVQYNLIGSHDA
                     PRPRTVLAGDIAALRLATLLPLVLPGTPAIYYGDELGMEGGPDPDCRRGYPVTPDETA
                     LTMRTFVRSAVHARRDHVALRQGTVAVAAAGGQAIALSREADGERALVAINSGREPAR
                     LDVDPALLAGLAPIALPEVAAGSVVGGGTLELPAQGALVLA"
     gene            complement(2961..>4286)
                     /gene="glgP"
                     /locus_tag="VHM48_09435"
     CDS             complement(2961..>4286)
                     /gene="glgP"
                     /locus_tag="VHM48_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2755674.1"
                     /translation="VSRDSVFTIHTPVSAGNERFAVDLVRRVAGPLLDGDGRPRTGGV
                     PIDRILDIGLGPDGDRSEFDMTGFSLRLTNDANAVSLLHAQTADATWSGTAPHRIRGI
                     TNGIHVPSWIGSAMGETLARDLNADLDDLDEETEVGPFWDKLADIPTEDLWETHQREK
                     LELAIFARRRLRSQFARHGEAPTVLDEVESALDPEILTIGFARRFATYKRAGLLFSDL
                     DRLARILWHEERPVQVVFAGKAHPADRPGQQVIQEIFGRSRSERLRGRVFILEDYDIR
                     IARYLVAGVDVWLNTPRRPLEASGTSGMKAAMNGAVNVSVLDGWWDEGWVGDNGWAIG
                     NRETNPDDGAQDWSDAQDLYRILEDEIVPRYYDRDERGLPSAWVDLMRRSMASALWRF
                     STTRMLHDYVEKMYLPAAASARTAAAEVAGAAAEKRAATRAAIVANQGG"
BASE COUNT          603 a         1518 c         1522 g          643 t
ORIGIN      
        1 gcgggggatc gcggtgatca gcgacctcgt cctcaaccac acgtccgacc agaacccgtg
       61 gttcaccgat tcgctggccg ggaccggcca cgaggactgg tacatctggt cggccacgaa
      121 cccgggctac ggcgggcccg acggccagca ggtctggcac ccgggcggca agcgctggta
      181 ttacggccag ttcggggccg gcctgccaga cctgaacctg cgtaacccgg cggtgaccgc
      241 cgagatggag gcggttgccc ggttctggat caccaccgtc gggttcgacg gcttccgtct
      301 cgatgcgatc aagcacctga tcgaggacgg ccagacccag gtcaacacgc ccgagaccca
      361 cgcctggctg caggccttcc gaacgacgct gcacgacacc gccccgaacg ccctcctcgt
      421 cggcgaggtc tacgacctgg cggaggccgt cgccccgtac gttcccgagg acgtcgacat
      481 ggccttcgac ttcggccttg cacaggcgac gatcgactcg atccggcgcg gcgatgccgg
      541 cccgctgaac ggagcgttcg ccgaggatgc ccggctcttc ctgccggacg aaggcgccag
      601 cttcctcacg aaccacgatc agtcgcggat cgccagccag ctccgcgagg atccggcggc
      661 gctccgactc gccgccggtc tcctcttcac cgacgccagg acgccgttcg tgtactacgg
      721 cgaggagatc gggctgaccg gcgagaagcc cgacgaagag atccggacgc cgctggcctg
      781 gaccggccag gctccggccg ccggcttcac gaccggcacg ccgtgggagt cgctcgcacc
      841 cggctgggag acgcgcaacg tcgccgccga ggctgccgat ccgacgtcgc tcctgtcggc
      901 gtaccgcgac ttgatccgcc tccacgacgg ccatcgcgcg ctgtgggcgg gatcgatcga
      961 accgctccgg gcgtccgact cggcggtcgc ggcctggctg cgggtcgggc aaggcgagca
     1021 gctggccgtg gtggccaacc tgtcggagca gcccgtgacc ggggcgacac tcgacctcga
     1081 ccgcgggccc ctctgcgggg aatcgaccgc gcgggtcctg taccagggcg gtgtcgctga
     1141 tccgggcggg gtcgcgctga cccccgcggc catcgacgcc gacggcgggt tccgcaactg
     1201 gacgccgctg accgtgctcc cggctcggag catcaccgtc atctcgctgg gaggctgaac
     1261 cgcgtgtccg tcgacgttcc ggcatgggcg gccgatgccg tcttctacca ggtcttcccg
     1321 gaccgcttcg ctcggagcgg acgggtcccc gcgcccggtc cgttcgaagc gtgggaggca
     1381 ccgccgacgc acgctggcta caagggcggc gatctgtacg gcgtcgtcga gcggctcgac
     1441 gagctggccg atctcgggat cagcgggctg tacctgaacc cgatcttcac cgccgcctcg
     1501 aaccaccgct acaacgcgtc ggactacctc acggtcgatc cgctgctcgg tgggaccgag
     1561 gcactccgag cgctcctcga cgctgcccac gggcgaggca tccgggtcct cctcgacggc
     1621 gtcttcaacc atgccggtcg tggcttcttc ccgttccagc acgtcatcga agcgggcgcg
     1681 gtctcgccct accgggactg gttctacctg gacccggacg tcctgagtgg gcaacgcggc
     1741 atcgacccgt acccgacggg cgatcccgag gggcatcatc tccgggccgg ctaccgttcg
     1801 tggtggaact ttccgtcgtt gccgaagctg cgggtcgagc atccgccggt gcgggagttc
     1861 ctgttcggcg tggccgagca ctggctccgc ttcgggatcg acggctggcg cctggacgtc
     1921 cccgccgaga tcgaggacga gaccttctgg ccagagttcc ggcagcgggt ccgggcggtc
     1981 cggtccgacg cctatctcgt cggcgaggtc tggtcggaat cgcccgagtg gctgaccggc
     2041 aaccggttcg acgcgttgat gaactaccca ctcgggctcg cgatcctcgg cttcgccagc
     2101 gccggccagc tcgaccaggc cgcgatcgac ggccaggccg actacaagcg gttcctccac
     2161 ccgatggacg gaccggcgtt cggcgaccga ctgcgccacc tgttcgccct gcacgatccc
     2221 gcgacgacct cggtccagta caacctgatc ggcagccacg atgcgccgcg gccccggacg
     2281 gtcctcgccg gggacatcgc cgcgctgcgc ctcgcgaccc tgctcccgct cgtcctcccc
     2341 gggactccgg cgatctacta cggcgacgag ctcggcatgg agggcggacc ggatccggac
     2401 tgccggcggg gctatcccgt caccccggac gagacggcgc tcacgatgcg gaccttcgtc
     2461 cggtcggcgg tccatgcccg gcgcgaccac gtggccctgc ggcaggggac ggtcgcagtc
     2521 gcggccgcgg gtggccaggc gatcgctctc tctcgcgagg ccgacggcga gcgagccctc
     2581 gtcgcgatca acagcggccg cgagccggct cgactcgacg tcgatcccgc gctgctcgcg
     2641 ggcctggcgc cgatcgcgct acccgaggtg gccgcaggga gcgtcgtggg gggcgggacg
     2701 ctcgagcttc cggcgcaggg cgccctggtc ctggcctgag gctcgcccgc ggcgggaccg
     2761 ccacgcggtc gacgttccca tacgccagtc aagctggcgt atggcgcggt cgatcgggac
     2821 ccgggaccga agtcactccc atacgtcggc cggacgaagc agatgggaag gtttcagggc
     2881 aggacagcga tccgccgttg gcttgtgcca cccgggctgt cgcatgggaa gaaccggggc
     2941 gcgggggacg agccgcgagc tcagccgccc tggttggcga cgatcgccgc gcgggtcgcg
     3001 gcgcgcttct cggccgcggc accggccacc tcggcggcgg ccgtgcgagc ggacgccgcg
     3061 gccggtaggt acatcttctc gacgtaatcg tggagcatcc gggtcgtcga gaaccgccac
     3121 agggccgacg ccatggagcg ccgcatcagg tcgacccagg ccgacggcag gccgcgctcg
     3181 tcgcggtcgt agtagcgggg cacgatctcg tcctcgagga tccggtagag atcctgtgcg
     3241 tccgaccagt cctgggctcc gtcgtcgggg ttcgtctccc gattgccgat cgcccagccg
     3301 ttgtcgccga cccagccctc gtcccaccag ccgtcgagga cgctgacgtt gacggcgccg
     3361 ttcatcgccg ctttcatccc gctcgtcccc gacgcctcga gcggtcgacg gggggtgttc
     3421 agccagacgt cgaccccggc cacgaggtag cgggcgatcc ggatgtcgta gtcctcgagg
     3481 atgaacaccc ggccgcggag tcgctcggag cgcgatcggc cgaagatctc ctggatgacc
     3541 tgctgaccgg gccggtcggc gggatgggcc ttgccggcga agacgacctg gaccggccgc
     3601 tcctcgtgcc acaggatgcg ggccagtcga tcgagatcgg aaaagaggag gccggcccgc
     3661 ttgtaggtcg cgaaccgtcg ggcgaagccg atcgtgagga tctcgggatc gagcgcggac
     3721 tcgacctcat cgaggaccgt cggcgcttcg ccgtggcggg cgaactggct gcgcaggcgg
     3781 cgccgggcga agatcgcgag ctcgagcttc tcgcgctggt gggtctccca gaggtcctcg
     3841 gtcgggatgt ccgcgagctt gtcccagaac ggcccgacct cggtctcctc gtcgaggtcg
     3901 tcgagatcgg cgttgaggtc gcgtgcgagc gtctcgccca tcgcgctgcc gatccaggag
     3961 gggacatgga tgccgttcgt gatgccccgg atccggtgtg gcgcggtccc gctccaggtc
     4021 gcgtcggcgg tctgggcgtg gagcaggctc accgcgttcg cgtcgttggt gagccggagg
     4081 gagaagccgg tcatgtcgaa ctcggagcgg tcgccgtccg ggccgaggcc gatgtcgagg
     4141 atgcgatcga tcgggacgcc gccggtcctg ggccgaccgt cgccgtcgag gagcgggccg
     4201 gcgacgcggc ggacgagatc gactgcaaac cgctcgttgc cggccgagac cggggtgtgg
     4261 atcgtgaaga cgctgtcgcg cgagac
//
LOCUS       DATAIW010000304         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_18987, whole genome shotgun sequence.
ACCESSION   DATAIW010000304 DATAIW010000000
VERSION     DATAIW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18987"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /gene="murD"
                     /locus_tag="VHM48_09440"
     CDS             complement(<1..1486)
                     /gene="murD"
                     /locus_tag="VHM48_09440"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2755675.1"
                     /translation="MTLIGPIDLDRLSLGAIRTGALHGRPVTVLGLARSGLALARFFA
                     DAGARVTIYDGRSAADLGDAIAALGERRMILALGPDVDPATTWADADLVATSPSITPD
                     FPTTEPRLREALRALVAARAAGGDAAPALVSEADLFLRLCPAPTVGVTGTKGKTTTAS
                     LIAAILAADPAHPVVLGGNIGIPLVERLFELVPDDRVVDELSELQLPTLSRGTTVAVY
                     TNVTADHLDRHGSLEGYRRVKRRLAELVDRDGALVLNDQDQVVARYADIGSARTILYR
                     RDRPRPGGLGVVDGWIVADGVERLALSGGGVAATGLDGRIMPVAELAIPGAHNVSNAL
                     AAIAVGLLFGVEPTAIRAAASAFTGVEHRLEPVAVIDGVRFINDSQGTQPDAVAAALR
                     AFDRPIVLIAGGRAKGIDLGALAPVVVERAYAAVLIGESGPDLEAGFRAAGLARTERA
                     PDLEAAVRRADAIAREALAAAPGAGAGAGAMSATVRLSPAAASSD"
     gene            complement(1492..2475)
                     /gene="mraY"
                     /locus_tag="VHM48_09445"
     CDS             complement(1492..2475)
                     /gene="mraY"
                     /locus_tag="VHM48_09445"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2755676.1"
                     /translation="MTVELIQGLLLAFALIVILMSPYIRLLHVLGFGKQIRREGPESH
                     YIKEGTPTMGGLLIVAVVLGIYFFLRQGPDSATFAPMAALAGVGALGVFDDYLNAKTG
                     EGIRARQKMIWLTVVAFVAAWQIQDTYNITAIPVPFVGPVSIDPWVYVAFGTFAIVAA
                     ANGVNITDGLDGLSGGTLAIAFVAYMLIALLNEPFAQPNLALLCALIIGALLGFLWFN
                     VHPAQIFIGDSGALSLGATLAVTALITGQILVLPLIGIIFVIETSSVILQVGYFKVSG
                     GKRLFRMSPIHHHFELGGWDEEKITIRFWIVGILAALLGVTLFLASLDRLR"
     gene            complement(2472..2975)
                     /locus_tag="VHM48_09450"
     CDS             complement(2472..2975)
                     /locus_tag="VHM48_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2755677.1"
                     /translation="MSSTRVTADEIAAGLGGGWSAPHRVQLVRLGAVTLVDDTYNASP
                     RSMVAALDLLAGLPGRRGAVLGEMLELGDASDEGHRVVGEAAARTVDWLVVVGSGAAG
                     IAEGARAAGMDPATVVRVRDADVALEAIPPRLRDGDVVLLKASRGVALDRVVDGLRRE
                     LGDGAAR"
BASE COUNT          496 a         1101 c         1142 g          385 t
ORIGIN      
        1 tgtcggagct cgcagccgcg gggctgagcc ggaccgtcgc cgacatcgca cccgccccgg
       61 cgcccgcacc cggcgccgcg gcgagcgcct cgcgcgcgat cgcgtccgcg cgacggacgg
      121 cagcctcgag gtccggcgcg cgttccgtgc gagccaggcc ggcggcacgg aacccggcct
      181 cgaggtccgg gccgctctcg ccgatgagca cggcggcgta ggcgcgctcg acgacgaccg
      241 gagccagcgc gccgagatcg atccccttgg ctcgaccgcc ggcgatcagg acgatcggcc
      301 ggtcgaaggc gcgcagcgcg gccgccaccg cgtcgggctg ggtcccctgc gagtcgttga
      361 tgaatcggac gccgtcgatg accgcgaccg gctcgagccg atgctcgacg cccgtgaatg
      421 ccgacgccgc cgcccggatg gccgtcggct cgaccccgaa cagcaggccg accgcgatcg
      481 ccgcgagggc gttggaaacg ttgtgcgccc ccgggatggc gagctcggcc acgggcatga
      541 tccgaccgtc caggccggtc gcggcgaccc cgccgccgct gagggcgagc cgctcgacgc
      601 cgtccgcgac gatccagccg tccacgactc cgagaccgcc gggacggggc cggtcgcgcc
      661 ggtagaggat cgtccgcgcc gacccgatgt cggcgtaccg ggcgacgacc tggtcctgat
      721 cgttgaggac cagcgccccg tcccgatcga cgagctccgc caggcgccgc ttgacccgcc
      781 gatagccctc gagcgacccg tgtcggtcga gatggtccgc ggtgacgttc gtatatacgg
      841 ccacggtcgt gccgcgcgac agcgtcggga gctggagctc cgagagctca tcgacgaccc
      901 ggtcgtccgg caccaactcg aacagccgct cgaccagcgg tatcccgatg ttgccgccga
      961 ggacgaccgg gtgggccgga tcggcggcca ggatcgccgc gatcagcgag gcggtcgtgg
     1021 tcttgccctt cgtccccgtc acgccgacgg tcggggcggg acacaggcgc aggaagaggt
     1081 ccgcctcgga caccagcgcg ggggcggcgt cgccgccggc ggcacgggca gccacgagcg
     1141 cgcgcagagc ctcacgcagg cgcggctcgg tcgtcgggaa gtcgggcgtg atcgacggtg
     1201 acgtcgcgac caggtcggca tcggcccagg tcgtggccgg gtccacatca ggacccagcg
     1261 cgaggatcat ccttcgctcg ccgagcgccg cgatggcgtc gccgagatcc gccgcggacc
     1321 ggccgtcgta gatggtcacg cgggcgccgg catcggcgaa gaaccgggcc agggcgagac
     1381 cgcttcgggc gagaccgagc acggtgaccg gccggccatg caaggcgccg gtgcggatgg
     1441 cgcccaggct gaggcgatcg aggtcgatcg ggcctatcag tgtcatcgtc gtcaccgcag
     1501 ccgatccagc gaggccagga agagcgtgac gccgaggagc gcggcgagga tcccgacgat
     1561 ccagaagcgg atcgtgatct tctcctcgtc ccagccgccc agctcgaaat gatggtggat
     1621 cgggctcatc ctgaagagac gcttgccgcc cgacaccttg aagtagccga cctgcaggat
     1681 gaccgacgac gtctcgatca cgaagatgat gccgatgagc ggcagcacga ggatctggcc
     1741 ggtgatgagg gcggtgacgg ccagggtcgc ccctaacgac agggcgcccg aatccccgat
     1801 gaagatctgc gccgggtgca cgttgaacca gaggaacccg aggagtgccc cgatgatcag
     1861 ggcgcacagg agggccaggt tcggctgggc gaacggctcg ttgagcaggg cgatgagcat
     1921 gtacgcgacg aaggcgatgg ccagcgtgcc cccggacagc ccatcgagcc cgtcggtgat
     1981 gttcacgccg ttggcggccg ccacgatcgc gaaggtcccg aaggcgacat acacccaggg
     2041 gtcgatcgag accggcccga cgaacggcac cgggatcgcc gtgatgttgt aggtatcctg
     2101 gatctgccat gccgcgacga aggcgacgac ggtcagccag atcatcttct gtctggcgcg
     2161 gatcccctcc ccggtcttgg cgttgaggta atcgtcgaat acccccaggg ccccgacacc
     2221 ggcaagcgcg gccatgggcg cgaacgtcgc ggagtccggt ccctggcgga ggaagaagta
     2281 gatgcccagg acgaccgcca cgatgagcag gccgcccatc gtcggcgtgc cctccttgat
     2341 gtagtggctc tccgggccct cccggcggat ctgcttgccg aacccgagga cgtgcaggag
     2401 ccggatgtac ggcgacatga ggatgacgat cagggcgaac gcgaggagca ggccctggat
     2461 gagttcgacc gtcatcgcgc ggcaccatcc ccgagctcgc gacgcaggcc gtcgacgacc
     2521 cggtccaggg ccacgccgcg ggaagctttg agaaggacga catcgccgtc gcgcagccgt
     2581 ggcgggatcg cctcgagggc gacgtcggca tcgcggacgc ggacgaccgt ggccgggtcc
     2641 atgccggccg cccgggcgcc ctcggcgatc cctgctgcgc cggacccgac gacgacgagc
     2701 cagtccacgg tccgggcagc cgcctcgccc accacgcggt ggccctcgtc gctggcatcg
     2761 ccgagctcga gcatctcgcc caggaccgcc ccgcggcggc cgggcaggcc agccagcagg
     2821 tccagcgcgg cgaccatcga ccggggcgag gcgttgtagg tgtcatccac cagggtcacg
     2881 gccccgagac ggacgagctg gacacggtgg ggcgccgacc agccgcctcc gaggccggcc
     2941 gcgatctcat cggccgtgac cctggtggat gacacctaca acgcctcgcc acggtcgatg
     3001 gtcgccgcgc tggacctgct ggccggcctg cccggccgcc gcggggcggt cctgggcgag
     3061 atgctcgagc tcggcgatgc cagcgacgag ggccaccgcg tggtgggcga ggcggctgcc
     3121 cgga
//
LOCUS       DATAIW010000305         9705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_79192, whole genome shotgun sequence.
ACCESSION   DATAIW010000305 DATAIW010000000
VERSION     DATAIW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79192"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..501
                     /locus_tag="VHM48_09455"
     CDS             268..501
                     /locus_tag="VHM48_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755678.1"
                     /translation="MNGPTPIGPTLIGPESAWFVWSPDGAALLWLEVRALGSEAFRST
                     IQSIDRDFRQAPVTLRTVDGLIVCTPSWQRLEP"
     gene            564..1217
                     /locus_tag="VHM48_09460"
     CDS             564..1217
                     /locus_tag="VHM48_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755679.1"
                     /translation="MPKLVRATTYLAAALVVAACSSGSPAARPSSSPASAASPPASTV
                     AATAPLIPAPSVAPSAAASPTVFTSKTYGYSVMLPAGWTGVQATAVWNGTGAPGSDTP
                     EGDRFLGPAAPSSWALVTPKSKDLAGYAKEWVDATAKYHGDTCTAPPEAVDPIMIGSE
                     PATLLSFNCGILINGAVTVHKGIGYQFGFRDPSVQAATDPADRMTFLELLRSVQFPD"
     gene            1300..1905
                     /locus_tag="VHM48_09465"
     CDS             1300..1905
                     /locus_tag="VHM48_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755680.1"
                     /translation="MLDHEEKFLFKEEILHLANAVAEPGTLDNVEDLLTDVITSPRFD
                     SEVFTLERSLQVMLGPRAGTTLVGLLTVAPEVFEEVAEVRIPSEVHERLVAWRARFGL
                     AIDNALNFLNNPDGIQGHNFATQVAHVEERRVLQGRLTLVRYNNEPQFFQADASVFLG
                     LVTDVMERLGPYLEPDAVDAETLSRLRDAVALIERKTASRG"
     gene            1943..2380
                     /locus_tag="VHM48_09470"
     CDS             1943..2380
                     /locus_tag="VHM48_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755681.1"
                     /translation="MTSPSSNCDICRGEAFDAEFGRTLVWQDHLWRLSTSIRGPVVGF
                     SYLEPRRHIAHITDLDGSEAATFGSVLAKVTTLLRGEAGAELVYAMIFGERVAHLHVN
                     LAPHRAGDALTGGAGIVNLAAPAPTPGAQQAFVDRLRVAAASL"
     gene            2456..3646
                     /locus_tag="VHM48_09475"
     CDS             2456..3646
                     /locus_tag="VHM48_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755682.1"
                     /translation="MRQLYLAATGQNRGKTTASLGLFDGFLRAGLRTAFMKPVGQRTV
                     IDHGQPADEDAVLMHEIFPLAADYGAMSPVHIPRGFTKAYIAGEVVEDLGARIVAARD
                     TFRADHDILLIEGTGHAGVGAVIGLSNAVVASMLGAPAIIVSEGGVGRPIDEIVLNAS
                     LFAAHGVSVAGAIVNKVDLDAQPGIEQVLERGLALHGIRLLGVLPVRPILSNPTLGMV
                     LEGVGGETIHAGPDLDRVIDGVAIGAMEPSHMLQRVGRGTLVIVPGDREDVILTLTTA
                     HLARRFRIAGATEAARIAQIAAEATNERTANVVGHEGAAIGLILTGGYRPRDAVLAAI
                     RQADLFATIVPEDTYVVASEVHDLLVKTHPADHEKISLIKALVAEHLDIDRILAVATE
                     VPTA"
     gene            complement(3767..4756)
                     /locus_tag="VHM48_09480"
     CDS             complement(3767..4756)
                     /locus_tag="VHM48_09480"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX2755683.1"
                     /translation="MDEAPNRRALLAVAALLIGGAVLSSTVFLGGQTARILSTVGSSI
                     NNTGAGRGDSGETTDGDTTGSTGSGSDPAVGSANGGQVADAAATVPTLLIVRTGELEL
                     KVADLGAALRDGDAAVARAGGYISGSNRTAANGSESAQVTYRIPSAAWSSTLSALRGL
                     ASTVVKELVKTEEVTGQVVDLAARIANLRATEAALQAIMAKATKISDVLDVQEQLTAT
                     RGDIEKLVADKEHLVDRAAYGSLAVTYRLPVVPSPTATPVPANGWDPGDDVARASGKL
                     VRIGQTTTSIGIWLAIVGLPLAIGGLIVLFVSWQLYRLTRWLIGRRETTLREA"
     gene            complement(4842..5489)
                     /locus_tag="VHM48_09485"
     CDS             complement(4842..5489)
                     /locus_tag="VHM48_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755684.1"
                     /translation="MHPSFARRLAGFVAGLALLLSLGAPAALAKEGVEVTLVAPIPGD
                     ARPGDVVPVVFILATVSDSGAQPLRGSDVFFRLYGPTGAMTEAAGAEQSTKGTYKAMI
                     AIPAGGAARAEFGLHGSATDASGGTVDSDMIWTYDGVLVAGTAPVAVQPRFDPGPGSK
                     PAADPATTTSAPSTAPAGPSELDPRVPVVVGLAIGLAVAAGLAVGRRRRVDRLPA"
     gene            complement(5527..6432)
                     /gene="panC"
                     /locus_tag="VHM48_09490"
     CDS             complement(5527..6432)
                     /gene="panC"
                     /locus_tag="VHM48_09490"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2755685.1"
                     /translation="MTTTEGRATMPVVRTRAALRDALTTAGRPLGLVPTMGWLHAGHR
                     SLMQRARRDNATTIATIFVNPRQFNEAADYQRYPRNEARDLAICAEEGIDIVFAPGVD
                     EVYPPGFDTIVTVGAVARPLEGAARPGHFDGVATVVAILFDLIGADRAYFGQKDAQQA
                     MVIRQMARDLAIPIEVIACPTVREPDGLALSSRNVHLSPAERAAAPVLRRALLAARAR
                     WESGERSADALRAAMAEVLATESLATPAYVSVADERTLAELDHLADREPALLSLAVHF
                     GSTRLIDNESIDDGKTRGTTSRGPV"
     gene            complement(6594..7505)
                     /gene="panB"
                     /locus_tag="VHM48_09495"
     CDS             complement(6594..7505)
                     /gene="panB"
                     /locus_tag="VHM48_09495"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2755686.1"
                     /translation="MSATSEIVRRLTVGDIAKLHADGQRIAMLTAYDYPTAKLLDEAG
                     IPLLLVGDSLGRAMLGYENEIPVSMADMLHHTAAVTRGAKRALVVGDMPFLSYATADA
                     AVANAGRFLSGAGAQAVKLEGGVRTARTIEAIVKAGIPTMGHIGWTPQAANATGKVRV
                     LGKNREQARTLLHDALAVQEAGAFAVVLELVPEQLAGAITDRLRIPTIGIGAGAGCSG
                     QVQVITDLLGIETWHPKHSKPYADLRGTILAAAKAYAEDVAAGTFPGPEQTVRMDDVV
                     LDEVLGRGASDRGMEVMPEGIPLDRDL"
     gene            complement(7502..8878)
                     /gene="coaBC"
                     /locus_tag="VHM48_09500"
     CDS             complement(7502..8878)
                     /gene="coaBC"
                     /locus_tag="VHM48_09500"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX2755687.1"
                     /translation="MNPGAPIDGAEQEGADGRLAGRLIAVGVTGSIAAYKAADLVRRL
                     RDEGADVVVLMTPTATRFVGELTFAALTRHPVETDVGGGLLEDQRIGHIVIADSADAI
                     VIAPATAHWLAAMATGLAGDAVTAACLATAAPIVVAPAMDGEMYDHPATRANVARLRD
                     VFGYRIVEPEAGPLASGQSGRGRLAETAAIVDAVVAAVADRPVRAPDPTDRPPVVAEA
                     HDADLEGRHLVISAGGTAEPIDPVRFIGNRSTGKMGVALAQAALDRGARVSLVIGQIS
                     VALPSSSRMLVFKAETTAEMGGLVRAAVAEADALIMAAAVADFRPRQTAATKLGREMG
                     LTLELEPTEDILAGVAAAVPPVGDPTRPILVGFAAETGSLDRAADKLHRKGVDFLVAN
                     DVAEEGSGFGTDTNHVWILSADGEHVDLPLRSKRDVADEILDRVVAALDARDAAAQTG
                     PVTQETIA"
     gene            complement(8875..>9705)
                     /locus_tag="VHM48_09505"
     CDS             complement(8875..>9705)
                     /locus_tag="VHM48_09505"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2755688.1"
                     /translation="PYHRRMLLAVDIGNTNVTTGLFRNGSLAATRRAATDPRASADEL
                     EHLLDALLHLDDASFADVSAIACCSVVPALTAALETVAERRERPMLVAAVGTIPIAIR
                     VDRPAEVGADRLVNALAAARIHGTPAVVADFGTATTIDCVAADGAFVGGAIAPGLELG
                     LEALAARTARLPRVELRAPDRAIGRDTVSAIQSGTIFGYQALADGLIARIRGELAEAA
                     GVEPGDVRVILTGGLSAAPWARALVGVDAIDPDLTLKGLAILHAEVGGGQPLELGLAT
                     "
BASE COUNT         1382 a         3468 c         3449 g         1406 t
ORIGIN      
        1 gatgggatga cctggtcacc tgacgggcgt ttcattgcgt acctcggcct gccggatcgg
       61 ccggagacct cgccgtcggc ccaaatcaac cccgcggtcg gttccccgac tcctgacctc
      121 ttcctgatcg gtgccgacgg acgcggtgag cggaacctga ccagcacgcc cgcctccgaa
      181 agccaacctg agtggtcgcc agacggtacc tcactggcct tcgtgaccgc tgcggacgga
      241 gcaccccagc gcttgacgat catccacatg aacgggccga ccccgatcgg gccgactctc
      301 atcggccccg agtccgcgtg gttcgtgtgg tccccggatg gggcggcgct tctgtggctc
      361 gaggtccgcg cgctcggatc cgaggcattt cggagcacga ttcaatcgat cgatcgggac
      421 tttcgccagg cgccggtgac cctccggacg gtggacggcc tgattgtctg caccccgagc
      481 tggcaacggc tcgaaccgtg agggatcccg gagatgcatt cccggatctc gcctgcggca
      541 tccgccgccc agtggagaaa ctgatgccga agctcgttcg cgcgaccacc tatctggccg
      601 ccgcccttgt cgtcgccgca tgctccagcg ggtccccggc cgctcggcct tcgtcgtcgc
      661 ccgcgtcggc tgcgtcgccg cctgcgtcaa ccgtcgccgc cacagcgcct ttgattccgg
      721 ctccgtccgt ggcgccgagt gcggcagcga gccccacggt gttcacctcg aagacctacg
      781 gctactctgt gatgttgccc gcgggctgga ccggcgttca ggccacggcc gtctggaacg
      841 gcacgggggc accgggaagc gacactcccg aaggcgacag attcctcggc cccgccgccc
      901 caagttcgtg ggcgctggtg acgcccaagt cgaaggatct ggcgggctac gcgaaagagt
      961 gggttgacgc cactgccaaa taccacggcg acacgtgcac tgcgccaccg gaggcagtgg
     1021 atccgatcat gatcgggagc gagccggcga cgctcttgag cttcaactgc gggatcctca
     1081 tcaacggagc cgtcacggtc cacaagggca tcggctacca gttcgggttc cgcgacccct
     1141 ccgtgcaggc cgccacggat ccggccgatc gcatgacgtt tctggagctt ctcagatcgg
     1201 tccagtttcc ggattgaccg cgccatgtcc gagccgtttg gccggactcc agcagcgcgc
     1261 tggggtctcg ccgatcgggt ggctgctacc atccggacca tgctcgacca cgaagagaag
     1321 ttcctgttca aggaggagat cctccacctc gcgaacgcgg tggcggagcc cggcaccctc
     1381 gacaatgtcg aggacctcct gaccgacgtc atcacgagcc cgcgcttcga ctccgaggtg
     1441 ttcacgctcg agcgcagcct ccaggtgatg ctcggcccgc gagcgggcac gaccctcgtc
     1501 gggctgctga ccgtcgcgcc ggaggtcttc gaggaggtcg ccgaggtgcg catcccgtcg
     1561 gaggtccacg agcggctcgt cgcctggcgg gcgcgcttcg ggctcgcgat cgacaacgcc
     1621 ctgaacttcc tgaacaaccc cgacgggatc cagggccaca acttcgcgac ccaggtcgcc
     1681 cacgtcgagg agcggcgcgt cctccagggc cgcctgacgc tcgtccgcta caacaacgag
     1741 ccgcagttct tccaggccga cgcgagcgtg ttcctcgggc tcgtgaccga cgtcatggag
     1801 cggctcgggc cgtatctcga accggacgcg gtcgatgccg agacgctgtc gcgactccgc
     1861 gacgcggtcg ccctcatcga gcgcaagacg gccagtcgcg gttgacgtgg acgaccgcgg
     1921 gcgcgcgccc cgtcgtcagt ccatgacgag tccgagctcc aactgcgaca tctgccgcgg
     1981 cgaggccttc gatgccgagt tcggtcgcac gcttgtctgg caggaccacc tgtggcggct
     2041 gtcgacgagc atccgcggac cggtcgtcgg cttttcgtac ctcgagccgc gtcgccacat
     2101 cgcccacatc acggacctcg acgggtccga ggccgcgacc ttcggatcgg tgctggcgaa
     2161 ggtgaccacg ctgctccgcg gcgaggccgg ggcggagctg gtctacgcga tgatcttcgg
     2221 cgaacgcgtc gcccacctcc acgtgaacct ggccccgcat cgagcgggcg acgcgttgac
     2281 cggcggcgcc ggcatcgtca acctggccgc gcccgcccca accccgggcg cgcagcaggc
     2341 gttcgtcgac cgcctccgcg tcgccgcggc gtcgctctga ccgtgcgcca gtcctcgggc
     2401 cggcttcgcg gagctcagcc ttgaacctcg gagagcgaga gccggaaggc cgcccatgcg
     2461 tcagctttat ctagcagcca caggccagaa ccggggcaag acgaccgcct cgctcgggct
     2521 gttcgacggc ttcctgcggg caggcctgcg gaccgcgttc atgaagccgg tcggccagcg
     2581 gaccgtcatc gaccacggtc agccggccga cgaggacgcg gtcctcatgc acgagatctt
     2641 cccgctcgcc gccgactacg gcgcgatgag cccggtccat attccccgcg gcttcacgaa
     2701 ggcgtacatc gccggagagg tcgtcgagga cctcggggcg cggatcgtcg ccgcccgcga
     2761 taccttccgg gccgatcacg acatcctcct gatcgaaggc accggccatg ccggcgtcgg
     2821 ggcggtcatc gggctctcca acgcggtcgt cgcgtcgatg ctcggcgcgc cggcgatcat
     2881 cgtgagcgag ggcggcgtcg gtcggccgat cgacgagatc gtcctcaatg cttcgctgtt
     2941 cgcggcccac ggcgtgtcgg tcgccggggc gatcgtcaac aaggtcgatc tcgacgccca
     3001 accgggcatc gagcaggtcc tcgagcgcgg cctcgcgctc cacggcatcc gcctcctcgg
     3061 cgtcctgccg gtccggccga tcctgtcgaa cccgaccctc gggatggtcc tcgaaggcgt
     3121 cggcggcgag acgatccatg ccggaccgga tctcgatcgg gtgatcgacg gcgtcgcgat
     3181 cggggcgatg gaaccgagcc acatgctcca gcgcgtcggg cggggcacgc tcgtcatcgt
     3241 ccccggcgac cgcgaagacg tgatcctgac cctgacgacg gcccatctcg cccgtcgctt
     3301 ccggatcgcc ggcgcgaccg aggcggcccg gatcgcccag atcgccgccg aggcgacgaa
     3361 cgagcggacc gccaacgtcg tgggccacga gggcgccgcc atcgggctca tcctgaccgg
     3421 tggctaccgg ccgcgcgacg ccgtcctcgc ggcgatccgc caggccgatc tcttcgcgac
     3481 gatcgtcccg gaggacacct atgtcgtcgc gtcagaggtc cacgacctgc tcgtcaagac
     3541 gcatccggcc gaccacgaga agatctcgct gatcaaggcg ctcgtggcgg agcatctcga
     3601 catcgaccgg atcctggcgg tcgccaccga agtgccgacc gcctgagccg gcgacgatcg
     3661 gcccgcgcct cggccgtccg cctgtgcccc tggcgtgcaa ccggacgatc ggcccgcgcc
     3721 tcgacggtcc gcatgtgcct atggagcaca gtccggctcg ctcgactcag gcctcgcgga
     3781 gcgtcgtttc ccgtcgcccg atcagccagc gggtcagccg gtacagctgc cagctcacga
     3841 acaggacgat gaggccgccg atcgcgagcg gcaggccgac gatcgcgagc cagatcccga
     3901 tcgaggtcgt cgtctggccg atcctgacga gcttgccgct cgcccgagcc acgtcgtcac
     3961 cgggatccca gccgttcgcc ggcacgggcg tggcggtcgg cgagggcacg accggcaacc
     4021 ggtaggtgac ggcgaggctg ccatacgccg cccgatcgac gagatgctcc ttgtcggcca
     4081 cgagcttctc gatgtcgccg cgggtcgcgg tcagctgctc ctggacgtcg aggacgtcgc
     4141 tgatcttcgt cgccttcgcc atgattgcct ggagggcggc ctcggtcgcg cggaggttgg
     4201 cgatccgggc cgcgagatcg acgacctgcc cggtgacctc ctcggtcttg accagctcct
     4261 tgacgacggt cgaggcgagc ccgcgcaggg cgctcaaggt ggacgaccag gcggcgctcg
     4321 ggatccggta ggtcacctgg gccgactcgc tgccgtttgc ggccgtccgg ttcgagccgc
     4381 tgatgtagcc gcccgcgcgg gcgaccgcgg catccccgtc gcggagggca gcgccgagat
     4441 cggccacctt cagctcgagc tcgccggtgc ggacgatgag gagggtcgga accgttgcgg
     4501 cggcgtccgc gacctgcccc ccgttcgctg agccaaccgc gggatcggag cccgagcccg
     4561 tcgagcccgt cgtgtcaccg tccgtcgtct cgcccgagtc accccggccc gcgcccgtgt
     4621 tattgatcga ggatccgacc gtcgagagga tccgcgctgt ctggccgccc aggaagacgg
     4681 tcgaggacag gaccgctccg ccgatcagca gcgcggcgac ggccaggagg gctcggcgat
     4741 tcggggcttc atccatgggc cgatcgtcga cggtggcggc gcgctcgacc agcgatgaaa
     4801 cccctcaacg cgcgggccgg gacgccccag ccggccgacc gtcaggcggg caagcggtcg
     4861 acgcggcgcc gtcgaccgac cgccaggcca gccgcgacgg cgagaccgat ggccaggcca
     4921 acgacgactg gcacccgcgg atccagctcg ctcggaccgg ccggcgcggt cgatggggcg
     4981 gacgtcgtcg tcgccggatc cgcggcgggc ttcgagccgg gccccgggtc gaagcgcggc
     5041 tggaccgcaa cgggagcggt ccccgcgacg aggaccccgt catacgtcca gatcatgtcc
     5101 gaatccacgg tcccgcccga ggcatcggtc gcggacccgt ggaggccgaa ctcggcgcgg
     5161 gcggccccac cggccgggat cgcgatcatc gccttgtagg ttcccttcgt cgactgctcg
     5221 gcaccggccg cctcggtcat cgcaccggtc ggcccgtaca gccggaagaa cacgtccgac
     5281 ccgcgcagtg gctgcgcgcc gctgtcgctg acggtggcca ggatgaagac gaccgggacg
     5341 acgtcgccgg gccgggcatc acccgggatc ggcgcgacca gggtgacctc gacgccttcc
     5401 ttggccagtg cggccggcgc gccgagggag agcagcaggg cgagccccgc aacgaaaccg
     5461 gccagacggc gggcgaatga ggggtgcatc ggggcctccg acctcgtgat gatggtggtg
     5521 ccctccctac accggtccac gactggtggt tccacgggtc ttgccgtcgt cgatcgattc
     5581 gttgtcgatc aggcgggtcg atccgaaatg gacggcaagg gacaggagcg cgggctctct
     5641 gtcggcgaga tggtcgagct cggcgagagt tcgctcgtcg gcgacgctga cgtaggcggg
     5701 ggtggcgagc gattcggtgg ccaggacctc ggccatcgcc gcgcgcaacg cgtccgcgga
     5761 tcgctccccg gattcccagc gggcccgggc cgcgagcagc gcccgacgca ggacgggtgc
     5821 ggcggcgcgt tctgcagggg agaggtggac gttgcgtgac gagagggcca gcccgtcggg
     5881 ctcgcgcacg gtcgggcagg cgatcacctc gatcgggatg gccaggtcgc gagccatctg
     5941 gcggatcacc atcgcctgct gggcgtcctt ctggccgaaa taggcgcgat cggctccgat
     6001 caggtcgaac aggatcgcga ccacggtcgc gaccccgtcg aaatggccgg gccgcgcggc
     6061 gccctcgagc ggccgggcga cggccccgac ggtcacgatc gtgtcgaagc ccggcggata
     6121 gacctcatcg acgcccggcg cgaacacgat gtcgatgcct tcctcggcgc agatggccag
     6181 gtcgcgcgcc tcgttccgcg gatagcgctg gtagtcggcc gcctcgttga attggcgcgg
     6241 gttgacgaag atcgtcgcga tcgtcgtcgc gttgtcccga cgggcgcgct gcatcaggga
     6301 gcggtgcccg gcgtgcagcc agcccatcgt cgggaccagc ccaagcgggc gaccggccgt
     6361 ggtgagggcg tcgcggaggg cggctcgcgt ccgcacgacc ggcatcgtcg ctcggccttc
     6421 ggtggtcgtc acctgctgag gctacggcat cgggaccggg gcgcgccaag acggcgaggg
     6481 cccgccgccc gagggaggtc gatcaaaggt tgcgatcgag cggaattcct cgggccgatg
     6541 gcttcgcgga gctcagcctc ggacctcgga gggtcacgat gatgcgggtc gcctcacagg
     6601 tcacgatcga gcgggattcc ttcgggcatg acctccatgc cgcgatcgga ggcgccgcgg
     6661 ccgagcacct cgtcgaggac cacgtcgtcc atccggacgg tctgctccgg acccgggaat
     6721 gtgcccgcgg ccacgtcctc ggcataggcc ttcgcggccg cgaggatcgt gccgcgcagg
     6781 tcggcgtacg gcttcgaatg cttcgggtgc cacgtctcga tcccgagcag gtccgtgatc
     6841 acctggacct ggccgctgca gccggcgccg gccccgatcc cgatcgtcgg gatccggagg
     6901 cggtcggtga tcgcgccggc cagctgctcg gggacgagct cgagaacgac cgcgaaggcg
     6961 ccggcctcct ggaccgcgag tgcgtcgtgg agcagcgtcc gggcctgctc ccggttcttg
     7021 ccgaggaccc ggaccttgcc cgtcgcgttg gcggcctgcg gcgtccagcc gatgtgaccc
     7081 atcgtcggga tcccggcctt gacgatcgcc tcgatcgtcc gcgcggtccg gacgccgccc
     7141 tcgagcttca cggcctgggc gcccgccccg gagaggaagc gcccggcgtt ggcgacggcg
     7201 gcgtccgcag tcgcatagct caggaacggc atgtcgccga cgacgagggc gcgcttcgcc
     7261 ccgcgggtca cggccgcggt gtgatggagc atgtcggcca tcgagaccgg gatctcgttc
     7321 tcgtagccga gcatcgcccg gccgagcgaa tcgccgacga gcagaagcgg gatgccggcc
     7381 tcgtcgagga gcttcgcggt cgggtagtcg taggcggtca gcatcgcgat ccgctgcccg
     7441 tcggcatgga gcttggcgat gtcgccgacg gtcaggcgac gaacgatctc ggaggtggca
     7501 ctcacgcgat ggtctcctgc gtcacgggtc cagtctgcgc ggcggcgtcg cgcgcgtcca
     7561 gcgccgcgac gacgcggtcg aggatctcgt cggccacgtc ccgcttcgag cgcaacggca
     7621 ggtcgacatg ctcgccgtcc gccgagagga tccagacgtg gttcgtgtcg gtcccgaacc
     7681 cggagccctc ctcggcgacg tcgttcgcga ccaggaagtc gacgcccttc cgatgcagct
     7741 tgtccgcggc ccgatcgagc gagccggtct ccgccgcgaa cccgacgagg atcggccggg
     7801 tcgggtcgcc gaccggcgga accgcggcgg ccactccagc gaggatgtcc tcggtcggct
     7861 ccagctcgag ggtcagcccc atttcgcggc cgagcttcgt ggcggccgtc tggcgcggcc
     7921 ggaaatcggc gaccgcggcg gccatgatca gggcgtcggc ctcggcgacc gcggcccgga
     7981 cgagaccacc catctcggcg gtggtctcgg ccttgaagac gagcatcctc gagctcgacg
     8041 gcaaggcgac gctgatctgg ccgatgacga gactgacccg ggccccgcga tcgagcgccg
     8101 cctgggccag ggcgaccccc atcttgccgg tcgagcgatt gccgatgaac cggaccgggt
     8161 cgatcggctc ggcggtgccg ccggcgctga tcacgaggtg gcggccctcg aggtccgcgt
     8221 catgggcctc ggcaacgacg ggcgggcggt cggtcgggtc gggcgcccgg acgggccggt
     8281 ccgccaccgc cgccaccacc gcatcgacga tcgccgccgt ttccgccagc cggccccgac
     8341 cgctctggcc gctggcgagc gggcccgcct cgggctcgac gatccggtag ccgaacacgt
     8401 cgcggaggcg ggcgacgttc gcccgggtcg cagggtggtc gtacatctcg ccgtccatcg
     8461 ccggggccac gacgatcggg gcggccgtcg cgaggcaggc ggccgtgacc gcgtcgccgg
     8521 cgaggcccgt cgccatcgcg gcgagccagt gggcggtggc cggggcaatg acgatcgcgt
     8581 cggcgctgtc ggcgatcacg atgtggccga tccgctgatc ctcgaggagg ccgccgccga
     8641 catcggtctc gaccgggtgc cgggtcagcg ccgcgaacgt cagctcgccg acgaagcgcg
     8701 tcgcggtcgg ggtcatcagg acgacgacgt cggcgccctc gtcgcgcagg cggcgaacga
     8761 gatcggcggc cttgtaggcg gcgatcgagc ccgtgacgcc gaccgcgatc aggcggccgg
     8821 ccaggcggcc gtcggcgccc tcctgctcgg cgccgtcgat tggcgcaccc gggttcatgt
     8881 cgccagcccg agctcgagcg gctgtccgcc gccgacctcg gcgtggagga tggcgaggcc
     8941 tttgagcgtc aggtccgggt cgatcgcgtc gacaccgacg agggcccgtg cccacggtgc
     9001 cgccgacagg ccgccggtca ggatcacccg gacgtcgccg ggctccacgc cggccgcttc
     9061 cgcaagctcg ccgcggatcc gcgcgatcag cccatcggcg agggcctggt agccgaagat
     9121 cgtgccgctc tggattgcgc tcacggtgtc gcggccgatc gcccggtcgg gcgctcgcag
     9181 ctcgacccgt ggcagcctgg cggtccgtgc cgcgagcgcc tcgaggccca gctccaggcc
     9241 gggcgcgatc gccccgccga cgaacgcccc atcggcggcc acgcaatcga tcgtcgtggc
     9301 cgtcccgaag tcggcaacca ccgccggcgt gccgtggatc cgggccgcgg cgagggcgtt
     9361 gaccaggcgg tcggctccga cctcggccgg ccggtcgacc cggatcgcga tcgggatcgt
     9421 gccgacggcc gcaacgagca tcggccgttc gcggcgctcg gcgacggtct cgagggcggc
     9481 ggtcagcgcc gggacgaccg agcaacaggc gatcgcggag acgtccgcaa aggacgcgtc
     9541 gtcgaggtgg agcagcgcgt cgagcaggtg ctcgagctcg tcggcggacg cccggggatc
     9601 ggtcgcggcc cggcgggtgg cggcgagaga cccgttccgg aacaggccgg tcgtcacgtt
     9661 cgtgttgccg atgtccactg cgagcagcat gcgtcgatga tacgg
//
LOCUS       DATAIW010000306        13312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_187218, whole genome shotgun sequence.
ACCESSION   DATAIW010000306 DATAIW010000000
VERSION     DATAIW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187218"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1155
                     /locus_tag="VHM48_09510"
     CDS             481..1155
                     /locus_tag="VHM48_09510"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX2755689.1"
                     /translation="MAISLFLGGWLVMVAAMMLPASLPTIRQIEGVAAAFTRPRRVPA
                     SFLVAFAAVWALFGLAAFLGDMVVHHVVDTTPWLGERPYLIEAAILALAGLYQFAPLK
                     RRTLGECRDPGALVSAAALARGAARLGLRHGLACLGTSWALMLVMFGEGFGGLGWMVV
                     LTAVMVDETTGRRGHRTATVAGIVLLIAALSVLSSGTAHSAAAGSTRGAGMGSAPLAA
                     SATMGA"
     gene            1152..1505
                     /locus_tag="VHM48_09515"
     CDS             1152..1505
                     /locus_tag="VHM48_09515"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HEX2755690.1"
                     /translation="MNAPVQLAIRFLLELAAVVAAGVVGASIGSPPFGLVGGIALAAV
                     FVVAWGLFLAPKARFPQPATVRLMVGTLVMEVPAIGLAIVGSTLAGAVLAAAILANAV
                     ALAATGATDAEALFR"
     gene            1502..2419
                     /locus_tag="VHM48_09520"
     CDS             1502..2419
                     /locus_tag="VHM48_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2755691.1"
                     /translation="MTRPLRVGIQLPEVEREVRWPELVDLARTIEDAGFDAIWVGGHL
                     LYRWPDRPSRGPWEAWTMMAGLAAATSRVSIGPLVACTNFHNPALLAKQAATVDEIAG
                     GRFVLGLGAGWNVTEFGAFGFAYDHRVDRFEEAFTIIRTLLQEGQVDFDGRFYHARDC
                     ELLPRPRPGGPPLLIGSNGPRMLRIAAPHVQAWNTWYADTDNTPDGVAPLGEIVDAAC
                     RDVGRDPAAIARTVAVHVRMPGGQGRTMGGTTTTERVVPLEGSPEAIADGLRAYAAVG
                     VSEVQVVLDPIDRPSVERFAAVLPILDRG"
     gene            2634..3155
                     /locus_tag="VHM48_09525"
     CDS             2634..3155
                     /locus_tag="VHM48_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755692.1"
                     /translation="MTPDPHLKEPVAADDIYRDIGRGGVKISSVNANLGQGNPDIIKQ
                     INADLGGWPLRIVEYRSSAVLTRALAWTPGEAPGGDEAPYAFAGLNILITFGPISARA
                     PSVPEPVRARAAAALVAVLDPLLWPLAQRSVAAIPSRTPSPVATPTPTAKPTVKPTAR
                     PTAKPTAKPTKKP"
     gene            3152..3637
                     /locus_tag="VHM48_09530"
     CDS             3152..3637
                     /locus_tag="VHM48_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2755693.1"
                     /translation="MTTARRQGGRPGEDVSDLDKRIIEHLQQDGRRPFTQIALELGVS
                     EAAVRARTNRLVERGILQVVGVTDPLKLGFQQMAMIGIRCEADHLMDVAAKCAEMPEV
                     DYVVITAGTYDILIETVCEDNEGLLRFLAERLRTIEGVRDTETFVYLRMVKQSYQWGT
                     R"
     gene            3778..5124
                     /locus_tag="VHM48_09535"
     CDS             3778..5124
                     /locus_tag="VHM48_09535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2755694.1"
                     /translation="MPVEPVQAQTLILLAVVANLLVMAAVVVPPLLGRRGPLQTGSGA
                     GNAGERKTAELAAVLGEAGDVDLTDIGVVPPGAYDRVVRIVSWVFILATSTIVAVTGL
                     WRETQPAVFALLAAAGLFVLVVHDLLPPGALGTAKFVLEGSVAVTFATLLVVLTGREA
                     SPFFFTFPLIVGGAALVVTPRVTFALAAAASTAYIVAIAPPGSGGLPPMTVAIVGINI
                     TALILLAYVAMVIAREQRRARDAAIRLSTIDNLTGLFNRTFFFAAVEREIARSARSGR
                     GFCLLMMDLDELKQINDRNGHFFGDRVLRGVGEVIRSGGRRIDTSARYGGDEFVVLLP
                     ETDPTGAFVLAEKIRLGVADLRVDVAGSMIQPSISIGVVSYPEDGRTSDELMITADTS
                     MYRSKHAGKNRVTGVPHVSGVPVMDETVVSPRAGEELVAVAGKPSKPRRKPADKSV"
     gene            5133..6455
                     /locus_tag="VHM48_09540"
     CDS             5133..6455
                     /locus_tag="VHM48_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX2755695.1"
                     /translation="MADPRHPHHATADDVTPRGFATRAIQAAHRLPVVDQAPTSVPIY
                     QTVTFSAADAEELGAVANREVPGYAYGRLDNPTVVAFAAAVAELEGAEAGFAFASGMG
                     AIHAALGTVLSAGDRVVATQASYGTTRAQLTGVFGRLGVTTEFVDVTDLAAVAVALAV
                     APTRVLYAETIANPTIVVADHVALADLAHRFGALYIVDNTFASPYLCRPAELGADLVV
                     ESATKYLAGHSDVMAGVVAGATSLVHRVRDFQVDTGASLDPHAAFLAMRGLSTLALRM
                     DRHSTTAASLGAWLEAQPGVSRVWYPTLPTHPQHEVAMRQLARGGGMLAFELAGGRAA
                     GAAFIDTLTIPVRTASLGSVFTIVSHPPSTTHRQLNDAALAAAGITAGLLRCSVGLED
                     LDDLVADFARALEAARSAGDLAAAATANAGEASMVRAGEPEPAEPTPV"
     gene            6488..7945
                     /locus_tag="VHM48_09545"
     CDS             6488..7945
                     /locus_tag="VHM48_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2755696.1"
                     /translation="MTTWAETARPPTPDVFERIGRAILRLFTSVDFAVVQIIGLSLLA
                     LVGMTIKQLPTFALRSAGDYANAMADLHARYDPAFGRGIVDALERLQLFHVFTSTWFT
                     VGLIVLVISIVICTLDRTPRLWRQSKDIRVVQPDAFYDPELLDRAALTGVEAGAVRAA
                     LRRQRFVVREAEVDGVQYLYGDRHRWTKLATLLSHMGLILFLVAGVVTWQFGDEQGLI
                     VAEGDTLTVQPIGTPGLLLVKNYDFEAPGFLETGQASDFTTDLGVFQNGTEIARKTIR
                     VNDPLEIGGYNFHENGFGAAPELLISDAAGKPLWDGPVPLTDMADGLPYGTLSVPGRD
                     IGLSLLLKRDADGIGVVIVAPYRMAGVETDGSPKIEYLDQSAIAVAAGEAQVPFGLDF
                     SVGVRRFSDYVLLIAKKDPGQGIVWTAFLLLIIGLAITFYLPRRRIWARIAPSGELRL
                     VARSDRYVDLEREFGLLLDDLVARRVVADRAAPAG"
     gene            7974..9569
                     /locus_tag="VHM48_09550"
     CDS             7974..9569
                     /locus_tag="VHM48_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2755697.1"
                     /translation="MATLEDLRAAVLPGARWLAAGPDVAPSTATSLDLAWVRLMRARV
                     PAFDALEAGDLAIVPRAALAIVAPDDESVGALVRGCIDARIGGIVLVAEAAIVAGASI
                     DAALDELVETAAQAGLPVLDAGPADPTAVERSAIGFLVNHRAELERQATILETQLEQV
                     ALGGGGPDRLAAAIAGFLGRPVAIEGRRGATLAVHVPPDVPGAAAAAAAYHGRQRTAA
                     ARIPLPIPGEPASSSGALALLGERAATELERVATARIAGLVALQLARDEALGRAVETA
                     RREPLPADGPPWVVIVARQAAAAAGPDAAGASGASGADRVDRPVDADGPGPAQGHRES
                     ERREALRRDLRSLAPARRLSLRGDALSVELRIVAALDAADPDGSAIGGRIGAFLGRIV
                     AVSRPFSDATGRSSAEADARATLEAAEVLASPPSVARADRLPAYRLLGNLHNLPDGPR
                     QARALLAPLLRGRPDVRRERLATLRAVLDLPGLAEAADALGVHRNTVAYRVRRIEEAT
                     GWRLADPELRFPLALALRLVQDD"
     gene            9717..11081
                     /locus_tag="VHM48_09555"
     CDS             9717..11081
                     /locus_tag="VHM48_09555"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2755698.1"
                     /translation="MVIVREEVDGAETIATATESGIRFVQLQFTDILGLVKAVTIPIH
                     QLEGSVRHGTWFDGSSIEGFTRIAESDQYLMPDMTTFREIPWQTASGPRGTARVICDV
                     FTPNGEPFVGDPRGVLRRQVERAKKLGFVVNTGPELEFFLFRRGEGGGIEPLPHDEAG
                     YFDFSTDLAQEVRQDMVDALEAFGIRVEAAHHEVAAGQHEIDFEYADALKTADNAITF
                     KFALKAIAQQHGLYATFMPKPIHGINGSGMHTHQSLWSIADGRNAFADPDNKYGLSDI
                     ARSYMAGILAHARGMIAILAPLVNSYKRLVPGYEAPTYLTWGRTNRSALIRVPKVSPG
                     KSIEGTRIEVRCPDPSSNTYLAFAAMIAAGLDGVERGLTLGEPVEESLFEMDAARIAE
                     KGIRELPGTLGEAIDELERDPVIGAALGDHVLSHYVSAKRAEWDEYRTQVSSWELEQY
                     LERF"
     gene            11105..13252
                     /locus_tag="VHM48_09560"
     CDS             11105..13252
                     /locus_tag="VHM48_09560"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX2755699.1"
                     /translation="MTARHTAISAISDHRVASTQNYLEHAGQAIYGQYVFHEGAQREY
                     LAKPIFNHLRRTIEGLEPFDEVIADAVAHGVKEWAMAHGATHYTHWFVPMTGSTAEKH
                     DSFLNPTSDGLTIAEFSGKNLIQGEPDASSFPSGGIRATFEARGYTAWDVTSPIFLQV
                     EPNGVTMTIPTAFVSYTGEALDHKIPLLRSQEALGKQALRVLRWFGNTTAARVFTNIG
                     PEQEYFLVDRRLAQQRPDLVLTGRTLFGAASPKGQELDDQYFGSIRERILAFMMDLDR
                     ELWRLGIPAKTRHNEVAPGQFEMAPVYEPTSVGSDHNMVVMSMMRRLALQHSLMFLIH
                     EKPFAGINGSGKHNNWSMGTDEGENLLDPGNDPHANAQFLAFLVAVIRAVNVHADLLR
                     ASIADAGQDHRLGANEAPPAIMSIFLGSQLEDVIEQLDRGATSGSKKGGSANLGVMSL
                     PVLPKDATDRNRTSPFAFTGNKFEFRAVGSSAPIYWPQTVLNTAVADSLKQLADELEK
                     LDEGDFAGLTSILSGIVGANKQVLFEGNGYSEEWHAEAALRKLPNNRTTVDALPSLTT
                     AKAKKLFAGFGVLSERELSARVEIYWERYVKVGNIEANCALDIARTMILPATARYIGE
                     LSSGSSASRGITEICEKVAGLADGLVDAIHALEHAQHEAHEAASVEVEARAFVDHVIP
                     AQNALREVVDELETLVSDELWPLPKYRELLFAY"
BASE COUNT         1890 a         4862 c         4511 g         2049 t
ORIGIN      
        1 cgcgcggatc ctgatttgcc caaggcgcgg tcgacgcgtc aggggacacc tgggacttcg
       61 gagaccatga ttctgcggat gctttggcac ggcaccagat gtggggtcag cccctgtgta
      121 cagcgcatgc aggcgcaagg aactcgtgtt actcggactt cgacgcccgc tctggccgcc
      181 gcgttcgcgt ctggtcgccg atgacgggcc acgcgctttc agccaacgac ggagattccg
      241 ccgcagttcg ccaagagggt cgggtctgta gctccacaag tacgatttcg ccaattcggg
      301 aacggacttt gacgccgcaa tctccgtcca tgcggcgtcc gtcacgccgt ctgttcgaga
      361 tcgccgccgc ggtcgcgggc gacggagacg taaccgccct gtcacctcct cctcctcctc
      421 ccgcggcgtc gcgggtctgc caacgacgtg gtcaagccgc gagctcggac ttcaggcggt
      481 gtggccatct cgctcttcct cggcggctgg ctcgtgatgg tcgcggcgat gatgctgcct
      541 gcctcgctgc cgacgatccg ccagatcgaa ggcgtggcgg ccgcgttcac ccggccgcgt
      601 cgcgttccgg cttcgttcct cgtggcattc gccgctgtct gggcgctgtt cgggttggcg
      661 gccttcctgg gcgacatggt cgtccatcac gtcgtcgata cgacgccctg gctgggggag
      721 cgtccgtacc tgatcgaggc cgcgatcctc gccctcgccg gcctctacca gttcgcgccg
      781 ctcaagcggc ggacgctcgg cgagtgtcgg gaccccggcg ccctcgtgtc ggccgcagcg
      841 ttggcccggg gtgcggcccg gctcggcctc cgccatgggc tggcctgcct cgggacctcc
      901 tgggcgctca tgctcgtgat gttcggcgag ggcttcggcg gactgggctg gatggtcgtc
      961 ctgaccgcgg tcatggtcga cgagacgact ggacgacgcg gccatcgaac cgcgacggtc
     1021 gccggcatcg tgctcctcat tgccgcgctg tccgtgctgt cgagcgggac ggcccattcc
     1081 gctgccgctg gctcgactcg gggtgcgggc atgggttcgg ctccgctcgc cgcttcggcg
     1141 acaatggggg catgaacgca ccagtccagc tcgcgatccg cttcctcctc gagctcgccg
     1201 cggtcgtggc cgccggtgtc gtcggcgcct cgatcggctc gccgccgttc ggactcgtcg
     1261 gcgggatcgc gcttgccgcc gtcttcgtcg tcgcctgggg gctgttcctc gccccgaagg
     1321 cccgcttccc ccagccggcg acggtccgac tcatggtcgg gacgctcgtc atggaggtgc
     1381 cggcgatcgg actggcgatc gtcggctcga cgttggccgg cgcggtcctc gccgcggcca
     1441 tcctcgccaa cgccgtcgcg ctggcggcca cgggcgcgac cgacgccgag gcgttgttcc
     1501 gatgacgcgc ccgttgcgag tcgggatcca gctgccggag gtcgagcggg aggttcgctg
     1561 gccggagctc gtcgatctgg cgcggaccat cgaggacgcc ggtttcgacg cgatctgggt
     1621 cggcgggcat ctcctctatc gctggccgga ccgaccgtcc cgtggcccgt gggaggcctg
     1681 gacgatgatg gccggactgg ccgccgccac gagccgtgtg agcatcggcc cgctcgtcgc
     1741 atgcacgaac ttccacaacc cggccctcct cgccaagcag gctgcgaccg tcgacgagat
     1801 tgccgggggc cggttcgtgc tcggactcgg cgccggctgg aacgtgaccg agttcggggc
     1861 gttcggcttc gcgtacgacc atcgggtcga ccggttcgag gaggcgttca cgatcatccg
     1921 gaccctgctc caggagggcc aggtcgactt cgatggccgc ttctaccacg cccgcgactg
     1981 cgagctgctg ccgcgaccgc ggccgggcgg gccgccgctc ctgatcgggt cgaacgggcc
     2041 gcggatgctg cggatcgccg cgccgcacgt ccaggcgtgg aacacctggt acgccgacac
     2101 ggacaacacg cccgatggcg tcgccccgct cggcgagatc gtcgacgccg cctgtcgcga
     2161 tgtcgggcgt gacccggccg cgatcgcccg gaccgtcgcc gtccatgtcc gcatgcccgg
     2221 cggtcagggc cggacgatgg gcggcacgac gacgaccgag cgggtcgtgc ctctcgaggg
     2281 ctcgccggag gccatcgccg acggcctccg ggcgtacgcc gcggtcggtg tgagcgaggt
     2341 ccaggtcgtc ctcgacccca tcgatcggcc gtcagtcgag cgattcgcag cggtccttcc
     2401 gatcctcgat cgaggctgat cgccgcacgg tcccagcgcg aaggtacttc tgtcaggggt
     2461 tcattcgctg cgaaggtcgt gctagcatcc cgccggcgat gcgacattcg aatcatgccg
     2521 gccgggggcg ccgactgccg atctgggcag ccggcctctt cgccgtgatc gtcgccggct
     2581 gtggcgtgtc cgccgatggt tcggctccgc cctcggccgc ccctacgccg gtcgtgaccc
     2641 cggatcctca tctcaaggag ccggtcgccg ccgacgacat ctacagggac atcggtcgcg
     2701 gcggagtcaa gatcagctcg gtcaacgcca acctcggcca gggcaacccg gacatcatca
     2761 agcagatcaa tgccgatctc ggcggctggc ctctgcgcat cgtcgagtac cgctcgtcgg
     2821 ccgtcctgac gagggcgctg gcctggacgc ccggcgaggc gcccggtggc gacgaggctc
     2881 cctacgcgtt cgccgggctg aacatcctga tcacgttcgg cccgatcagc gcccgcgcgc
     2941 cgtcggttcc agagcctgtt cgcgcgcggg ccgccgcggc gctcgtcgcc gtcctcgacc
     3001 cgctgctctg gccgctcgcc cagcggtccg tcgcggcgat cccgagccgg acccccagtc
     3061 cggtggcgac gccgacgccg accgccaagc cgaccgtcaa gccgaccgcc aggccgaccg
     3121 ccaagccgac cgccaagccg accaagaaac catgaccacg gcgcgccgcc agggcggtcg
     3181 gccaggcgag gatgtgtccg acctcgacaa gcgaatcatc gagcacctcc agcaggacgg
     3241 tcgccggccg ttcacccaga tcgccctcga gctcggcgtg tcggaggcgg ccgtccgcgc
     3301 ccgcacgaac cgactcgtcg agcgtgggat cctccaggtc gtcggcgtga ccgatccgct
     3361 caagctcggc ttccagcaga tggcgatgat cgggatccgc tgcgaggcgg accacctcat
     3421 ggatgtcgcc gccaagtgcg ctgagatgcc cgaggtcgac tatgtcgtga tcaccgcggg
     3481 cacgtacgac atcctgatcg agaccgtctg cgaggacaac gagggcctgc tccgcttcct
     3541 cgccgagcgt ctccgcacga tcgagggtgt ccgcgacacc gagacgttcg tgtacctgcg
     3601 gatggtcaag cagtcgtacc agtggggcac ccgctaggag ccggtgcgcc gcggcaccca
     3661 caatccccgt tcgaccgcgg gctccgacgg cacgaacgac ggctggagga cgtccgaccg
     3721 gctacgatgc ggccaacgcg gggcgtccgc cccggcgcgt catgacgagg tccgagcgtg
     3781 ccggtcgaac cggtccaggc ccagacgctg atcctgctcg cggtcgtcgc caacctcctg
     3841 gtcatggccg cggtcgtcgt tccgccactg cttgggcggc gcggaccgct ccagaccggg
     3901 tccggcgccg ggaacgccgg tgagcgcaag acagccgagc ttgccgccgt cctcggcgag
     3961 gccggcgacg tcgacctcac cgacatcggt gtcgtgccgc cgggcgccta cgaccgcgtc
     4021 gtccggatcg tgtcgtgggt cttcatcctg gcgacctcga cgatcgtcgc ggtcaccggg
     4081 ctgtggcgcg agacccagcc agcggtcttc gccctcctcg ccgccgcggg cctgttcgtc
     4141 ctggtcgtcc acgacctcct cccgccgggc gcgctcggga cggccaagtt cgtcctcgaa
     4201 ggcagcgtgg ccgtgacatt cgcgacgctc ctcgtggtcc tgaccggtcg cgaggcgagc
     4261 ccgttcttct tcacgttccc gctcatcgtc ggcggcgcag cgctcgtggt gacgccacgg
     4321 gtgaccttcg cgcttgcggc ggcagcgtcg accgcgtaca tcgtcgcgat cgcgccaccg
     4381 ggctccggcg gcctgccacc gatgaccgtc gcgattgtcg ggatcaacat caccgctctg
     4441 atcctgctcg cgtatgtcgc gatggtcatc gcccgcgagc agcggcgggc gcgcgacgcg
     4501 gcgatccggc tctcgacgat cgacaacctg accgggctct tcaaccggac gttctttttc
     4561 gcagccgtcg agcgcgagat cgcccggagc gcgcggtcgg gtcgcggctt ctgcctgctg
     4621 atgatggatc tcgacgagct caagcagatc aacgaccgca atggccactt tttcggcgat
     4681 cgcgtgctcc gcggcgtcgg cgaggtgatc cggtccggtg gccggcggat cgacacgtcc
     4741 gcccgctatg gaggcgacga gttcgtggtc ctcctgcccg agaccgatcc gaccggcgcc
     4801 ttcgtcctcg ccgagaagat ccggctcggt gtcgcggacc tgcgcgtcga tgtcgccggt
     4861 tcgatgatcc agccgtcgat ctcgatcggg gttgtcagct accccgagga cggccggacc
     4921 agcgacgagc tgatgatcac cgccgacacc tcgatgtacc gctcgaagca cgccgggaag
     4981 aaccgggtga cgggcgtccc gcacgtctcg ggggtcccgg tcatggacga gacggtcgtc
     5041 agtccgcgcg ccggcgagga gctggtggcg gtcgctggca agccgagcaa gcctcgccgg
     5101 aagccggccg acaagtcggt gtaggatcgg gcatggccga tccccggcac ccgcaccacg
     5161 ccaccgccga cgacgtcacg ccccgcggct tcgcgacccg ggcgatccag gccgcccatc
     5221 ggctgccggt cgtcgaccag gcgccgacct cggtgccgat ctatcagacg gtcaccttct
     5281 cggcggccga cgccgaagaa ttgggcgccg tcgccaaccg cgaggtgccg ggttatgcct
     5341 acggccgcct cgacaacccg acggtcgtcg cgttcgcagc cgcggtcgcc gagctcgagg
     5401 gcgccgaggc cggcttcgcc ttcgcgtccg ggatgggcgc gatccacgcg gcgctcggaa
     5461 cggtgctgtc ggccggcgac cgggtcgtcg cgacgcaggc ctcgtacggc acgactcgcg
     5521 cccagctgac cggcgtcttc gggcgcctgg gggtgacgac cgagttcgtc gacgtgaccg
     5581 acctcgcggc cgtcgcggtc gcgctcgcgg tggcgccgac acgcgtcctc tacgccgaga
     5641 cgatcgccaa cccgacaatc gtcgtcgcgg accatgtcgc cctcgccgat ctcgctcacc
     5701 ggttcggggc gctgtacatc gtcgacaaca cgttcgcctc gccctatctc tgccggccgg
     5761 ccgagctcgg cgcggacctc gtcgtcgagt cggccacgaa atacctcgcc ggccacagcg
     5821 acgtcatggc cggcgtcgtc gccggggcca cgagcctcgt ccaccgcgtc cgcgacttcc
     5881 aggtcgacac cggcgccagc ctcgatccgc acgccgcctt cctggcgatg cgcgggctct
     5941 cgacgctggc cctccggatg gatcgccact cgaccaccgc agcctcgctg ggggcgtggc
     6001 tcgaggcgca gcccggcgtc agccgcgtct ggtacccgac cctgcccacg catccgcagc
     6061 atgaggtggc catgcgccag ctggcacgcg gtggcggaat gctggctttc gagctggccg
     6121 gcggacgcgc ggcgggggcg gccttcatcg acaccctgac gatcccggtc cggaccgctt
     6181 cgctcggcag cgtcttcacg atcgtcagcc acccgccctc gacgacccac cgccagctca
     6241 acgacgccgc gctcgcggcc gccgggatca cggccgggct cctgcgctgt tcggtcggtc
     6301 tcgaggacct cgacgatctc gtggccgact tcgcgcgagc cctcgaggcg gcccggtccg
     6361 ccggggatct cgccgctgcg gcaaccgcca acgccggtga agcgtccatg gtccgggccg
     6421 gcgagcccga gccggccgaa ccgacccccg tctgaccttt ccggaggatc acccgatcgc
     6481 cagcccggtg acgacgtggg ccgagacggc ccggccgccc acgcccgacg tgttcgagcg
     6541 gatcggccgc gccatcctgc gcctgttcac ctcggtcgac ttcgcggtcg tccagatcat
     6601 cggcctgtcg ctgctggcgc tcgtcgggat gacgatcaag cagctcccga ccttcgcgct
     6661 ccgttctgcg ggcgactatg cgaatgcgat ggccgacctc cacgcccggt acgacccagc
     6721 gttcggccgg gggatcgtcg acgcgctcga gcggctccag ctgttccacg tcttcacctc
     6781 gacgtggttc acggtcgggc tgatcgtcct cgtcatctcg atcgtcatct gcaccctcga
     6841 tcgaacgccg cggctgtggc gccagtcgaa ggacatccgg gtcgtccagc ccgatgcgtt
     6901 ctacgacccg gagctgctgg accgggcggc gctgaccggc gtcgaggccg gcgcggtccg
     6961 ggctgcgctg cggcgccagc ggttcgtggt tcgggaggcg gaggtcgacg gcgtccagta
     7021 tctctatggc gatcgccacc gctggacgaa gctcgcgacg ctgctcagcc acatgggcct
     7081 gatcctgttc ctcgtcgccg gagtggtgac gtggcagttc ggcgacgagc agggcctgat
     7141 cgtcgccgag ggcgacacgc tcacggtcca accgatcggg acacccggcc tgctcctcgt
     7201 gaagaactac gacttcgagg cgccgggctt tctcgagacg ggccaggcgt cggacttcac
     7261 gaccgatctc ggcgtgttcc agaacggcac ggagatcgcc cgcaagacga tccgggtcaa
     7321 cgacccgctc gagatcggcg gctacaactt ccacgagaac ggcttcgggg cggcacccga
     7381 gctcctgatc agcgacgccg caggcaagcc gctctgggat ggcccggtgc cgctcaccga
     7441 catggccgac ggcctcccgt acggcacgct gtccgtcccg gggcgcgaca tcggattgag
     7501 cctcctgctc aagcgcgatg ccgatggcat aggtgtcgtg atcgtcgccc cgtaccggat
     7561 ggccggcgtc gagaccgatg gctcgccgaa gatcgaatac ctcgaccaga gtgcgatcgc
     7621 cgtcgccgcc ggcgaggccc aggtcccgtt cgggctcgac ttctccgtcg gtgtccggcg
     7681 attcagcgac tacgtgctgc tgatcgcgaa gaaggacccg gggcagggga tcgtctggac
     7741 cgcgttcctc ctgctgatca tcggccttgc catcacgttc tacctgccgc gccgacgaat
     7801 ctgggcccgg atcgccccgt ccggcgagct ccggctcgtg gcccgctccg atcgctatgt
     7861 cgacctcgag cgcgagttcg gcctgttgct cgacgatctc gtcgcccgcc gcgtcgtcgc
     7921 cgaccgcgcc gccccggccg gctgaccgcc gtctggcctg agacccgaat tcgatggcga
     7981 ccctcgagga cctgcgcgct gcggtcctgc ccggtgcccg ctggctggcg gccggccccg
     8041 acgtggcgcc gtcgaccgcg accagcctgg accttgcgtg ggtccgcctg atgcgcgccc
     8101 gggtgcccgc gttcgacgcg ctcgaggcag gcgacctcgc gatcgtgccg cgggctgccc
     8161 tcgcgatcgt cgcgcccgac gacgagagtg tcggagccct cgtccgcggt tgcatcgacg
     8221 cccggatcgg cggaatcgtc ctcgtggccg aggccgcgat cgtggcgggt gcctcgatcg
     8281 atgcagcgct cgacgagctc gtcgagacgg cggcccaggc cggcctgccc gtgctcgacg
     8341 ccgggccggc cgaccccacg gcggtcgagc gcagcgcgat cggcttcctc gtcaaccatc
     8401 gagccgagct cgaacggcag gcgaccatcc tcgagaccca actcgagcag gtcgccctcg
     8461 gcggcggtgg tccggaccgg ctggccgccg cgatcgcagg cttcctcggc cggccggtcg
     8521 cgatcgaggg ccggcgcggg gcgacgcttg ccgtccacgt cccgcccgat gtcccgggtg
     8581 cggctgccgc ggcggcggcc taccacggcc ggcaacgcac ggccgcggcc cggatcccgc
     8641 tgccgatccc cggcgagcct gcatcgagca gcggagcgct cgccctgctt ggcgaacgcg
     8701 cagccaccga actcgagcgg gtcgcgaccg cacggatcgc cggcctcgtc gccctccagc
     8761 tcgctcgcga cgaggccctc ggtcgagcgg tcgagacggc gcgacgggag ccgctgccgg
     8821 ccgatggacc gccatgggtc gtcatcgtcg cccgccaggc ggcggccgcc gccggcccgg
     8881 acgcagcggg cgcgtcgggc gcatcgggcg ccgaccgcgt cgatcgaccc gtggatgccg
     8941 acgggccggg accggcccag ggtcatcggg agtccgagcg acgcgaggcg ctccgccgtg
     9001 acctgcgttc ccttgccccg gcccgccggc tttccctccg cggtgacgcc ctcagcgtgg
     9061 agctccggat cgtggccgcg ctcgacgcgg cggacccgga cggatccgcg atcggcggac
     9121 ggatcggcgc cttcctggga cggatcgtgg ccgtgtcgcg gccgttcagc gatgccacgg
     9181 gccgatcgtc cgccgaggcc gatgcccggg cgaccctcga ggcggccgag gtgctcgcga
     9241 gcccgccgtc ggtcgcccgg gccgaccggt tgcctgcgta ccggttactg ggcaacctgc
     9301 acaacctgcc ggacggaccg cgccaggcac gcgccctcct cgccccgctc ctgcgcggtc
     9361 ggcctgacgt ccgtcgtgag cgtctcgcca cgctccgggc cgtgctcgac ctgcccggtc
     9421 tcgccgaggc ggccgacgcc ctcggcgtcc accgcaacac cgtcgcctat cgcgtccgcc
     9481 gcatcgagga ggcgacgggc tggcggctgg ccgacccgga gttgcggttt ccgctcgcgc
     9541 tcgccctcag acttgtgcaa gatgactaac ttgcgtgggg ccccgcaacc ttcaccgagg
     9601 cccaacccgc agctatcctg acacgacccg ttcgtgtcat gccggagtcg tgcgttccgc
     9661 acaacactcc cgccggcgat cggcaagcag cgaaccggat cgaggagcct ggatcggtgg
     9721 tcatcgtgcg cgaggaggtc gacggagccg agacgatcgc gacggcaacg gagagcggga
     9781 tccgattcgt ccagctccag ttcaccgaca tcctcggcct cgtcaaggca gtcacgatcc
     9841 cgatccacca gctcgaggga tcggtccgcc acgggacgtg gttcgacggc agctcgatcg
     9901 aggggttcac ccggatcgcc gagagcgacc agtacctcat gccggacatg accaccttcc
     9961 gcgagatccc ctggcagacg gcgtccggtc cgcgcgggac ggcccgcgtc atctgcgacg
    10021 tgttcacccc gaacggcgag cccttcgtcg gtgatccgcg cggcgtcctc cgccgccagg
    10081 tcgagcgggc gaagaagctc ggcttcgtgg tcaacaccgg gcccgagctc gagttcttcc
    10141 tcttccggcg cggcgagggc ggcggcatcg agccgctgcc gcatgacgag gcgggctatt
    10201 tcgacttctc gaccgacctc gcccaggagg tccgccagga catggtcgat gcgctcgagg
    10261 ccttcggcat ccgggtcgag gcggcccacc acgaggttgc cgccggccag cacgagatcg
    10321 atttcgagta cgccgacgcc ctgaagaccg ccgacaacgc gatcacgttc aagttcgcgc
    10381 tgaaggcgat cgcccagcag cacggcctgt acgcgacgtt catgccgaag ccgatccatg
    10441 ggatcaacgg ctcgggcatg cacacccacc agagcctgtg gtcgatcgcc gacgggcgga
    10501 acgccttcgc cgatccggac aacaagtacg gcctctccga catcgcccgc tcgtacatgg
    10561 cgggcatcct cgcccatgcc cgcggcatga tcgcgatcct ggccccgctc gtgaacagct
    10621 acaagcggct ggtcccgggg tacgaggcgc cgacctacct cacctggggg cggacgaatc
    10681 gctcggccct catccgggtg ccgaaagtgt cgccgggcaa gtcgatcgag ggcacccgga
    10741 tcgaggtccg ctgccccgat ccgtcgtcga acacgtacct cgccttcgcg gccatgatcg
    10801 cggccggcct cgacggggtg gagcgcggcc tgaccctcgg cgagccggtc gaggagagcc
    10861 tgttcgagat ggacgctgcc cggatcgccg agaaggggat ccgggagctg ccgggcaccc
    10921 ttggcgaggc gatcgacgag ctggagcggg accccgtgat cggagccgcc ctcggcgacc
    10981 acgtcctgtc gcactacgtg tccgcgaagc gggccgagtg ggacgagtac cgaacccagg
    11041 tatcgagctg ggaactggag caatacctcg aacggttctg atcacccgca ctgataggag
    11101 acccatgacc gcacgtcaca cggccatctc ggcgatctcg gaccatcggg tcgcgtcgac
    11161 ccagaactac ctggagcacg ccgggcaggc catctacggc cagtacgtgt tccacgaggg
    11221 cgcccagcgc gagtacctgg ccaagccgat cttcaaccac ctgcggcgga cgatcgaggg
    11281 cctcgagccg ttcgacgagg tcatcgccga cgcggtcgcg cacggcgtga aggaatgggc
    11341 catggcccac ggggccaccc actacaccca ctggttcgtg ccgatgaccg gctcgaccgc
    11401 cgagaagcac gactcgttcc tgaacccgac cagcgacggg ctgaccatcg cggagttctc
    11461 cggcaagaac ctgatccagg gcgagccgga tgcgagctcg ttcccgtccg gcgggatccg
    11521 ggccacgttc gaggcccgcg gctacaccgc ctgggacgtc acgagcccga tcttcctcca
    11581 ggtcgagccg aacggcgtca cgatgacgat cccgacggcg ttcgtctcgt acacgggcga
    11641 ggccctcgac cacaagatcc cgctcctccg ctcgcaggag gcgctcggca agcaggcgct
    11701 gcgcgtcctg cgctggttcg gcaacacgac ggccgcgcgc gtgttcacca acatcgggcc
    11761 ggagcaggag tacttcctcg tggaccggcg gctggcccag caacgaccgg atctcgtcct
    11821 gaccggtcgg acgctcttcg gcgccgcgtc gcccaagggc caggagctcg acgaccagta
    11881 cttcggctcg atccgcgagc ggatcctcgc gttcatgatg gatctcgacc gcgagctctg
    11941 gcggctcggg atcccggcca agacccgcca caacgaggtg gcgcccggcc agttcgagat
    12001 ggccccggtc tacgagccga cctcggtcgg ctccgaccac aacatggtgg tcatgagcat
    12061 gatgcggcgc cttgcgctgc agcacagcct gatgttcctg atccatgaga agccgttcgc
    12121 gggcatcaac ggctcgggca agcacaacaa ctggtcgatg ggcaccgacg agggcgagaa
    12181 cctcctggac ccgggcaacg acccccatgc caacgcccag ttcctggcgt tcctcgtcgc
    12241 ggtgatccgc gcggtcaacg tccatgccga cctgctccga gccagcatcg cggatgccgg
    12301 ccaggaccat cgcctcgggg ccaacgaagc cccgccggcg atcatgtcga tcttcctcgg
    12361 ctcacagctc gaggacgtca tcgagcagct cgaccggggg gcgacctcgg gatccaagaa
    12421 gggcggctcg gcgaacctcg gggtcatgtc gctgccggtc ctccccaagg acgccacgga
    12481 tcgcaaccgg acctcgccat tcgcgttcac cggcaacaag ttcgagttcc gcgcggtcgg
    12541 ctcgtccgcc ccgatctact ggccacagac ggtcctcaac accgccgtcg ccgactcgct
    12601 caagcagctg gctgacgagc tcgagaagct cgacgagggc gatttcgccg gcctgacgag
    12661 catcctttcc gggatcgtcg gggcgaacaa gcaggtgttg ttcgagggca acggctactc
    12721 cgaggaatgg catgccgagg ccgccctccg caagctgccc aacaaccgga cgacggtcga
    12781 cgcgctgccg tcgctgacga ccgccaaggc caagaagctg ttcgccggct tcggcgtcct
    12841 gtccgagcgc gagctcagcg ctcgcgtcga gatctactgg gagcggtacg tcaaggtcgg
    12901 caacatcgag gcgaactgcg ccctggacat cgccaggacg atgatcctgc ccgcgaccgc
    12961 ccggtacatc ggcgagctct cgagcggctc gtccgcgtcg cgggggatca ccgagatctg
    13021 cgagaaggtc gcaggtcttg cggacggcct cgtcgatgcg atccacgccc tcgagcatgc
    13081 gcagcacgag gcgcacgagg cggcctcggt cgaagtggaa gcccgggcgt tcgtggatca
    13141 tgtgattccg gcccagaacg ccctgcgcga ggtcgtggac gagctcgaga ccctggtctc
    13201 ggacgagctc tggccactcc cgaagtaccg cgagctcctg ttcgcctatt gaggtcgggg
    13261 gcgcggggcc ggccgcgccc ccgacctcaa taggcgaaca ggagctcgcg gt
//
LOCUS       DATAIW010000307         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_79238, whole genome shotgun sequence.
ACCESSION   DATAIW010000307 DATAIW010000000
VERSION     DATAIW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79238"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VHM48_09565"
     CDS             <1..1169
                     /locus_tag="VHM48_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX2755700.1"
                     /translation="PGSAGSGLASPSSEPGSSVAPTGTPTPSVGPAPSGDYDPAKFGF
                     AAKGMRGEVMAFVTTSQVDDALARLDFASTSTIAFFSLVAGANGSINRDTRWRSWNGP
                     LVDQLIERAHATGTKVVISLARFSWSASQTKTSATLLASAAARGRLAKAVVDEVVRRG
                     VDGVNVDFEPIPRGQKANFTDLVRRIRAELDGRGPGYQLTFDVVGHFESYDVGSALRP
                     GGADAVYLMGYHYAGTFSTIAHGTAPLGGSRYSVADAIRGLRKVARPGQLIVGVPYYG
                     HLWPTVSGALNARTTAGGYDVLYSSARLIGAKRGTTYDPVEQVQRVAWRGRSCATCPL
                     HWFQLYYDDARSLGAKWAEFRRQGLLGTGVWTSAFEGSSGELTAALRKVWLVGG"
     gene            1336..>1599
                     /locus_tag="VHM48_09570"
     CDS             1336..>1599
                     /locus_tag="VHM48_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755701.1"
                     /translation="MHSSLIGKVEKANRYARELDRITIDRLALTFRGDNDTHHVSLDA
                     GQWHCTCHYFDSWQTCVHVLTLQKIVGVMLPESAQESIFSAQAA"
BASE COUNT          233 a          554 c          562 g          250 t
ORIGIN      
        1 ggccgggatc ggcgggatcg ggcttggcct cgccgtcgag cgaacccgga tcgagcgtcg
       61 cgccgacggg gacaccgacg ccttcggtcg gccccgctcc gtccggcgac tacgacccgg
      121 ccaagttcgg gttcgccgcg aaaggcatgc gcggcgaggt gatggcgttc gtcacgacct
      181 cccaggtcga cgacgcgctc gcgcggctcg acttcgcttc gacgtccacg atcgcgttct
      241 tcagcctcgt cgcgggcgcc aacggatcga tcaaccgcga cacccgctgg cgaagctgga
      301 acggccccct cgtcgaccag ctgatcgagc gggcccacgc gacgggcacg aaggtcgtga
      361 tctccctcgc gcggttctcg tggtcggcca gccagacgaa gacctcggcc accttgctcg
      421 ccagcgccgc ggctcggggg cgtctggcga aggcggtcgt cgacgaggtc gtccggcgcg
      481 gcgtcgacgg ggtcaacgtc gacttcgagc cgatcccgcg cggccagaag gcgaacttca
      541 ccgacctggt ccgacggatc cgggccgagc ttgacggccg cggaccgggc taccagctga
      601 cgttcgacgt cgtcggccat ttcgagagct atgacgtcgg ttcggcgttg cggcccgggg
      661 gcgccgacgc ggtctacctg atgggctacc actacgccgg gacgttctcg acgatcgccc
      721 atgggaccgc gccgctgggc ggctcgcgct actcggtggc cgatgcgatc cgcggcctgc
      781 gcaaggtcgc caggccgggc cagctgatcg ttggcgtgcc gtactacggc cacctctggc
      841 cgacggtcag cggcgcgctc aacgcccgga cgaccgcggg cggctacgac gtcctctact
      901 cgagcgcgcg cctgatcggg gcgaaacgag gcacgaccta cgacccggtc gagcaggtcc
      961 agcgggtcgc ctggcgcggc cggtcctgcg cgacgtgccc gctccactgg ttccagctct
     1021 attacgacga tgcccgttcg ctcggcgcga agtgggccga gttccggcgc cagggcctgc
     1081 tcgggacggg ggtctggacg tccgcgttcg agggttcgtc gggcgagttg acggcggcgc
     1141 tgcggaaggt ctggctcgtc ggcggctgac tccgtcgtga cccgatcgtc acctggtcgg
     1201 aggcgtttcg ggggatgacc ggaggggcgt cggggtgtat ggtggcgcca cgaccgccac
     1261 tcaccggtcg ggtgcacgac cggatacggc ggcctcgtga atgcgccccc aacggggctg
     1321 cggaggtatg cgtcgatgca cagctccctg atcggcaagg tcgagaaggc aaaccgctac
     1381 gcccgcgagc tcgatcggat cacgatcgac cggctcgcgc tcacgttccg cggtgacaac
     1441 gacacgcacc atgtcagcct cgacgccggt cagtggcatt gcacctgcca ctacttcgac
     1501 agttggcaaa cgtgcgtcca cgtcctgaca ctccagaaga tcgtcggcgt gatgctgcct
     1561 gaatccgcgc aggaatcgat cttctcagcc caggctgcc
//
LOCUS       DATAIW010000308         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_19136, whole genome shotgun sequence.
ACCESSION   DATAIW010000308 DATAIW010000000
VERSION     DATAIW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19136"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1601)
                     /gene="clpX"
                     /locus_tag="VHM48_09575"
     CDS             complement(291..1601)
                     /gene="clpX"
                     /locus_tag="VHM48_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX2755702.1"
                     /translation="MTTSSTSKGPKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINL
                     CQEIIEEEMLEAPKAKPSVAKLPNPRQIKFALDQYVISQDRAKKVLSVAVYNHYKRVN
                     AGHAVDEVELQKSNVLLVGPTGSGKTLLAQTLARVLDVPFCIADATSLTEAGYVGEDV
                     ENILLRLIQSADFDVARAQRGIIYIDEIDKIARKADNPSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGGKHPHQDFIQIDTTNVLFVCGGAFDGLEKIVEERVGRRSIGFGSDAG
                     VSSAERGKILERLMPEDLLKFGLIPEFIGRLPVIVTLNTLTAEDLVRVLTEPKNAVTR
                     QFQKFLGLDKVELVFTSGALQAAADVALERKTGARALRSIVEEALLEVMYDIPERSDV
                     RKCIITEETIREGRLPLLLTKTEVDKGVDETNYAEWLAERGETA"
     gene            complement(1624..2427)
                     /locus_tag="VHM48_09580"
     CDS             complement(1624..2427)
                     /locus_tag="VHM48_09580"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX2755703.1"
                     /translation="MSMTAPPPTSRLRPADRTHASPSFETAGRLQMLVPMVIESSSRG
                     ERAYDIYSRLLRERIIFLGDAIEDHVANLIIAQLLFLDSEDPEKDISLYINSPGGVVT
                     SGLAIYDTMQYVRAPVATICIGMAASMAAVLLAAGAPGKRYALPNGRIMIHQGTGGFR
                     GAAPDAMIQMKEWEFLVQRNHEILANHTGQPLEKVTQDTNRDYFMSPDAAKDYGIIDY
                     VYTATGDSLIARAHDLGLSGGEGGSVPNAATEEEAAKQDEKSDGDKKGK"
     gene            complement(2381..>3528)
                     /gene="tig"
                     /locus_tag="VHM48_09585"
     CDS             complement(2381..>3528)
                     /gene="tig"
                     /locus_tag="VHM48_09585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX2755704.1"
                     /translation="FRPEIETTDEPKVDKVIEELRDQNASLSPVEERGAQKGDYAVIK
                     YEGTRDGVSFEGGSAERMPLIIGEDRLIPGFEDELVGLTIGDTKGFDITFPADYGEET
                     LAGQLARFEVEVRELREKILPDLDDDFARSMGDFTDLANLREEVAKRLQRNALDKARH
                     TFSDQIIEYAVANATIDLPDVLAEQEVEVMHDEFRSAMARQGISEEAYEKVSGKSHED
                     LHADFRPDAEKRVKVLLVLSKIAEAENLTIDDAAVEAEIAKGRERYAGDSKLTKYFES
                     DRGRNYIRSTLRRSRVVEKLVDDWLAAHPDHPAIPHVEDGPADTVDDEQARSVAAVGA
                     TDPGSILQADAHDLDHDHEHHDHDHGAHDPGHHEHDRAAADEPAPAG"
BASE COUNT          494 a         1167 c         1204 g          663 t
ORIGIN      
        1 accttggccc tggcggacct gctgggacca ggcggcgaga tcgtcgccat cgatcgcgac
       61 gcagccgccc tcgccaggct tcggtcgtgg ccggccggac gatggcgatg tcggcggaca
      121 cgatcgcggt tgccatggcc cgcgtcgtcg caccagcaac gcgtcccact cgtgaaggcc
      181 gtccgaggcc ctgcgccgcc ggcccgacgc cggagatcgt cggtgaggcg ggacccggtc
      241 gttccccggg gtcgcgtgcc gccgatcagc cgatcagccg atcacgagga tcaggccgtt
      301 tcgcctcgct cggcgagcca ctccgcgtag ttcgtctcgt cgacgccctt gtcgacctcg
      361 gtcttcgtca gcagcagcgg cagccgaccc tcacggatcg tctcctcggt gatgatgcat
      421 ttgcggacgt cggatcgctc ggggatgtcg tacatcactt cgaggagcgc ctcctcgacg
      481 atcgagcgga gggcacgcgc gccggtcttg cgctcgaggg cgacatcggc ggcggcctgc
      541 agcgcgcccg aggtgaagac cagctccacc ttgtcgaggc cgaggaactt ctggaactgg
      601 cgggtgaccg cattcttcgg ctcggtcagc acgcggacga ggtcctcggc cgtcagggtg
      661 ttcagggtga cgatgaccgg cagccggccg atgaactcgg ggatcaaccc gaacttcagg
      721 aggtcctccg gcatgagccg ctcgaggatc ttgccgcgct cggcgctcga cacgccggcg
      781 tcggatccga acccgatcga gcgtcgaccc acccgctcct cgacgatctt ctcgagaccg
      841 tcgaaggcgc cgccacacac gaacagcacg ttcgtcgtgt cgatctgaat gaagtcctga
      901 tgcgggtgct tcccaccgcc ctgcggcggg acgttggcga cggttccctc gaggatcttc
      961 aggagtgcct gctggacgcc ttcaccggac acgtcgcggg tgatcgaggg gttgtccgcc
     1021 ttgcgggcga tcttgtcgat ctcatcgatg tagatgatcc cgcgctgggc ccgggcgacg
     1081 tcgaagtcgg ccgactggat gagccgcaga aggatgttct cgacatcctc accgacgtag
     1141 cccgcctccg tcagcgacgt ggcgtcggcg atgcagaacg ggacgtcgag gacgcgggcg
     1201 agggtctggg cgaggagggt cttgcccgaa ccggtcggcc cgacgagcag gacgttggac
     1261 ttctggagct cgacctcgtc gaccgcgtgg ccggcattga cccgcttgta gtggttgtag
     1321 acggcgaccg agaggacctt cttggcccgg tcctggctga tcacgtactg gtcgagggcg
     1381 aacttgatct ggcggggatt cgggagcttg gcgaccgacg gcttggcctt cggcgcctcg
     1441 agcatctcct cctcgatgat ctcctggcag aggttgatgc actcgtcgca gatgtagacg
     1501 ccctgcccgg cgatcagctt ccggacctgc tcctggctct tgccacagaa ggaacagcgg
     1561 tacggaacct tggggccctt ggacgtgctg ctggtcgtca ccggtttctc gcgggctccc
     1621 tggctacttg cccttcttgt cgccgtcgga cttctcgtcc tgcttggccg cctcttcctc
     1681 ggtcgccgcg ttcggcacgc tgccgccctc gccgccgctg aggccgagat catgcgcccg
     1741 ggcgatcagc gaatcacccg tggccgtgta aacgtagtcg atgatgccat agtccttggc
     1801 cgcgtcggga gacatgaaat agtcccgatt cgtatcctgc gtcaccttct cgaggggctg
     1861 ccccgtgtgg ttggccagga tctcgtggtt gcgctggacg aggaactccc attccttcat
     1921 ctggatcatc gcatcggggg cggcgccgcg gaacccgccg gtgccctggt ggatcatgat
     1981 ccggccgttc ggcagggcgt agcgcttgcc cggtgccccg gccgccaaca gcaccgccgc
     2041 catcgaggcc gccatcccga tgcagatggt cgcgacgggc gcccggacgt actgcatcgt
     2101 gtcgtagatc gcgaggccgc tcgtcaccac gccaccgggc gagttgatgt agaggctgat
     2161 gtccttctcg gggtcctcgg agtcgaggaa caggagctgg gcgatgatca ggttcgcgac
     2221 gtgatcctcg atcgcgtccc cgaggaagat gatccgctcc cggagcagcc gcgagtagat
     2281 gtcgtaggcg cgctcgccac ggctgctgga ctcgatgacc atcggtacga gcatctggag
     2341 tctcccggct gtctcgaagg aagggctggc gtgggttcgg tcagccggcc ggagccggct
     2401 cgtcggcggc ggcgcggtca tgctcatgat ggccgggatc gtgggcgccg tggtcgtggt
     2461 cgtggtgctc gtgatcgtgg tcgagatcgt gcgcgtcggc ttgcaggatc gaccctggat
     2521 ccgtcgcccc gacggcggcc acggagcggg cctgctcgtc gtccaccgtg tccgccggtc
     2581 cgtcctcgac atgggggatg gccggatggt ccgggtgcgc ggcgagccag tcgtcgacga
     2641 gcttctcgac gacccggctg cgccgaagcg tcgagcggat gtagttccgg ccgcgatcac
     2701 tctcgaagta cttcgtgagc ttcgaatcgc cggcgtagcg ctcgcggccc ttggcgatct
     2761 cggcctcgac cgctgcgtcg tcgatcgtga ggttctcggc ctcggcgatc ttgctcagga
     2821 ccaggagcac cttgacccgc ttctcggcgt cgggccggaa gtcggcgtgg aggtcctcgt
     2881 ggctcttgcc cgagaccttc tcgtacgcct cctcgctgat cccctggcga gccatggcgc
     2941 tgcggaactc gtcgtgcatc acctcgacct cctgctcggc gaggacgtcg ggcaggtcga
     3001 tggtcgcatt cgcgacggcg tactcgatga tctggtccga gaacgtgtgg cgggccttgt
     3061 cgagggcgtt gcgctggagt cgcttcgcca cttcctcgcg gaggttggcg aggtcggtga
     3121 agtcgcccat cgagcgggcg aagtcatcgt ccagatcggg caggatcttc tcccggagct
     3181 cgcggacctc gacctcgaag cgggccagct ggccggccag cgtctcctcg ccgtagtcgg
     3241 cggggaacgt gatgtcgaag cccttcgtgt cgccgatcgt caggccgacg agctcgtcct
     3301 cgaagcccgg gatcaggcgg tcctcgccga tgatcagcgg catccgctcg gccgagccgc
     3361 cttcgaacga gaccccgtcg cgagtgccct cgtacttgat gaccgcgtag tcgcccttct
     3421 gcgccccgcg ctcctcgacc ggcgacaacg acgcgttctg gtcgcggagc tcctcgatga
     3481 ccttgtcgac cttcggctcg tcggtcgtct cgatctccgg gcggaaat
//
LOCUS       DATAIW010000309         5893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_246766, whole genome shotgun sequence.
ACCESSION   DATAIW010000309 DATAIW010000000
VERSION     DATAIW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246766"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VHM48_09590"
     CDS             complement(<1..557)
                     /locus_tag="VHM48_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755705.1"
                     /translation="MTSDPAFGPSAGPARLGVRRGLDACASVRGTFGVLALDHRQNLR
                     HELRPDEPAAVTYDELVEFKREVVRALAPVATGTLLDPEIGAAQCIADGSLPGGAGLI
                     VAIEATGYEGPATARVSRILDGWSVAKAKRMGASAAKLLVYHHPDAANAADQEALVAR
                     VAADCRDADLALFVEPLSFSLVEGE"
     gene            complement(554..1630)
                     /locus_tag="VHM48_09595"
     CDS             complement(554..1630)
                     /locus_tag="VHM48_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2755706.1"
                     /translation="MTGDGLRLDEYRPRSSLRVAAHVIRRPRFPVVDAHNHLGPAFGG
                     DWATRPPAELIATLNEAGVEMIVDLDGGQGDALSAEIERWQEAFPDRIAVFAGLDYDV
                     WRTDPDFGETEAGRLRDAARRGARGLKVWKLLGLRARDPAGRLVAVDDPRLDPLWATA
                     ADLDLPVVIHIADPIAFFEPLDPTNERWEELRDHPDWHFWPPRSGGQPDAPGFPSFND
                     LLAAFGRLVGRHPRTTFVGAHVGCAAEDLALVGELLDASPNLFVDIAARLGELGRQPY
                     TARAFFLRHADRILFGMDMAPDPELYAIHYRFLETFDESFDYSTEPVPGQGRWQIHGI
                     GLPDDVLRRVYRDNARRVLRLETA"
     gene            complement(1627..2706)
                     /locus_tag="VHM48_09600"
     CDS             complement(1627..2706)
                     /locus_tag="VHM48_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2755707.1"
                     /translation="MNAFENLVTWRESSTSGEAIAAALDARGPEQAAADAILAPAERI
                     VITGAGSSYYLAQVAAAAARASMGRSVIAVPLSELILRPDGVLVGVGRPGGVARPGLE
                     PVVIISRSGSTSEAVDVAERMRVAGHPTVAVTCRGQSPLADRADVTLVSRAGDETAIV
                     MTRSFASMLALLLDVVARLGQDDALSAGLRRIPDRWAEARAAAEVGRELGTTDWSRIV
                     ILGGGPAQGMAAEWGLKLTETSQVATNAFEPLEFRHGPISVCEPGMLVVGLVAGPGAA
                     EEVAVVEEAARLGASTWLIARDEGEARGTTGRLSLIGGGLHPSARLPLLAHPGHALAL
                     SLALTRGLDPDAPRHLDQVVILGAE"
     gene            complement(2703..3434)
                     /locus_tag="VHM48_09605"
     CDS             complement(2703..3434)
                     /locus_tag="VHM48_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2755708.1"
                     /translation="MSPAVAPGLPLYRRIEADVRARIRDGGLQPGAQVAPEPELMAEF
                     GVSRATVRQALAGLVAEGLLEIRRGLGTYVTAPRFEHTIGGFYSFSREIERHGLQPGT
                     RVLDLRTMPAADDVAEALGVAGGTRVVALRRLRLAGPDPLVVETSHLPAARFPRLETV
                     DFSTVRLYDTLLNAYGCRPTRARETFSPVLLTADEAALLDQRRGEPALRVERVAFDQD
                     DLPIEFCRSTVRGDRYRYSVELRDR"
     gene            complement(3431..4336)
                     /locus_tag="VHM48_09610"
     CDS             complement(3431..4336)
                     /locus_tag="VHM48_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2755709.1"
                     /translation="MSATAGAVPRPVAPARRGRRLRTARRAIFVGLLIGGGLLMMAPF
                     LWMISTSLKTRAEVFATPPTLFPIVPQWHNYPDMWNALPFATFFVNSFKLAALNTVGQ
                     LISCSMAAYAFAVLRFRFREPLFALLVVTLIIPFQVVLVPNFILYRLLPHPFSSSGNW
                     IGTQEPLWVGAFLGGAFGTFLLRQFFLAIPRELGDAARVDGANPWQIYRHIYLPLARP
                     ALATLAIFTFMWSWNDLIDPIIYLRDVDSLTVTAGLSLFQGQYVGRWPLLMAGALVSV
                     LPMIVLFIVAQRHFVRGIALTGIKG"
     gene            complement(4333..5244)
                     /locus_tag="VHM48_09615"
     CDS             complement(4333..5244)
                     /locus_tag="VHM48_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2755710.1"
                     /translation="MTPRSRPAPRRGGGPLAGEGRWALIFLAPTLIGLAVLSAGPILA
                     TLAISLTKWDLLTVPKLTGVDNYAALLGDDRFLKALRNTFFYTAVSVPVGLTIAFGLA
                     IALNAKIRGIAFIRTAYFLPVVTSTIAIALVWQWIYSADSGLLNEVLGLVGIHAQKWL
                     SDPTLAMPAIVAMSIWQGLGVNVIIFLAGLQAIPSDLLDAASVDGAGSWARLRRVTLP
                     LMTPSIFFTGVLSLIGSFQVFDQIFVLIKPRPTEATITVVYFIYENGFKFFKMGYASA
                     ASWILFLIVAVFTAIYFWSQKRWVHYQ"
     gene            complement(5273..>5893)
                     /locus_tag="VHM48_09620"
     CDS             complement(5273..>5893)
                     /locus_tag="VHM48_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2755711.1"
                     /translation="DIISADHKTSLVGSAEAAGGLQFLQDLIWKDKVMPDASITDALG
                     DAFEQGQAAMEANGSWLVATHIAAGLDFGIAPLPKGPAGQATSINPTGAVVYKGTKNP
                     DAAWAFVKYLASPAAQTQLMQLKASLPANKEVLAGPFATSFDGADVLAAAIDYAHLKP
                     SFKGFNDWTTKLQTEIDANVFTEPKKTAAQALADVVPSLDQILASQ"
BASE COUNT          964 a         1989 c         2154 g          786 t
ORIGIN      
        1 ttctcgccct cgacgaggga gaaggacagg ggctccacga acagggccag gtccgcgtcg
       61 cggcagtcgg cggcgacgcg ggcaacgagc gcctcctggt ccgccgcatt ggcggcgtcg
      121 gggtggtggt agacgaggag cttggccgcc gacgcgccca tccgcttggc cttcgcgacg
      181 ctccaaccgt cgaggatgcg gctcacccgg gcggtcgccg gcccctcgta tccggtggcc
      241 tcgatcgcca cgatcaggcc ggccccgccc ggcagcgagc cgtccgcgat gcattgggcc
      301 gcgccgatct ccggatccag gagggtgccc gtggcgacgg gcgcaagggc gcgcaccacc
      361 tctcgcttga actcgaccag ctcgtcgtac gtgactgccg cgggttcgtc cggacgcagc
      421 tcgtggcgga ggttctggcg atgatcgagg gcgaggacgc cgaacgtgcc ccggacggag
      481 gcacaggcat cgagcccacg gcgtacgccg agacgagccg gcccggcgga cgggccgaac
      541 gctggatcgg aggtcatgcc gtctccaggc ggaggactcg tcgggcattg tcgcgataga
      601 cccgacggag gacgtcgtcg ggcaggccga tcccgtggat ctgccagcga ccctgaccgg
      661 gaaccggctc ggtgctgtag tcgaacgatt cgtcgaaggt ctcgaggaat cggtaatgga
      721 tcgcgtagag ctcgggatcg ggcgccatgt ccatcccgaa caggatccgg tcggcatggc
      781 ggaggaagaa cgcccgagca gtgtagggct ggcggccgag ctcgccgagc cgggccgcga
      841 tgtcgacgaa gaggttcggg ctggcgtcga gcagctctcc caccagggcc aggtcctcgg
      901 ccgcacagcc gacgtgggcg ccgacgaacg tcgtccgcgg atggcgcccg acgagccgtc
      961 cgaacgcggc gagcaggtca ttgaacgacg gaaaccccgg ggcgtccggc tggccacccg
     1021 accgaggcgg ccagaagtgc cagtcggggt ggtcgcgcaa ctcctcccag cgctcgttgg
     1081 tcgggtcgag cggctcgaag aacgcgatcg gatcggcgat gtggatgacg acgggcaggt
     1141 ccaggtcggc ggccgtggcc cagagcgggt cgaggcgcgg gtcgtcgacg gccacgaggc
     1201 ggccggcggg atcgcgcgcc cgcaggccga gcagcttcca gaccttgagg ccgcgagcgc
     1261 cccgccgggc cgcatcgcgc agccggcccg cctcggtctc accgaagtcg gggtccgttc
     1321 gccagacgtc gtaatccagg cctgcaaaca cggcaatgcg atcggggaac gcctcctgcc
     1381 atcgctcgat ctcggccgac aacgcgtcgc cctggccgcc gtcgaggtcg acgatcatct
     1441 cgacaccggc ctcgttgagc gtcgcgatca gctcggccgg cggacgcgtt gcccaatcgc
     1501 cgccgaacgc cggcccgagg tggttgtggg cgtcgacgac ggggaaacgc ggccgacgga
     1561 taacgtgcgc cgccacgcgc agggacgacc gcggccggta ttcgtcgagg cgaaggccgt
     1621 cgccggtcat tccgcgccca ggatgacgac ctggtcgagg tggcgcggtg cgtcgggatc
     1681 gagaccgcgg gtcagggcaa gactgagcgc gagcgcatgg cccgggtggg ccaggagcgg
     1741 caaccgcgcc gacggatgca gcccgccgcc gatcaggctg agacgacccg tcgtgccgcg
     1801 ggcttccccc tcgtcccgcg cgatcagcca ggtcgacgcg ccgaggcgag ccgcctcctc
     1861 gaccaccgcg acctcctccg cggcaccggg tccggcgacg agaccgacga ccagcatgcc
     1921 cggctcgcag accgagatcg gaccgtggcg gaactcgagc ggctcgaagg cgttcgtcgc
     1981 gacctggctc gtctcggtga gcttcagacc ccactcggcg gccatcccct gcgccggccc
     2041 accgccgagg atcacgatcc ggctccaatc ggtcgtgccc agttcccgcc cgacctcggc
     2101 ggccgccctt gcctccgccc atcgatcggg aattcggcgg agaccggccg acagggcatc
     2161 gtcctggccg aggcgcgcga ccacgtcgag caggagggcg agcatgctgg cgaacgaccg
     2221 ggtcatgacg atcgccgtct cgtcgccggc ccgcgacacg agcgtgacgt cggcgcgatc
     2281 cgcgagcgga ctctgcccgc gacacgtcac cgccaccgtc gggtgccccg ccacccgcat
     2341 ccgctcggcg acgtcgacgg cttccgacgt cgagccggac cgggaaatga tcacgaccgg
     2401 ttcgagaccg ggccgggcga caccgcccgg ccgcccgacc ccgacgagca caccgtcggg
     2461 ccggaggatg agctcggaca atggcacggc gatcaccgag cggcccatcg acgcgcgggc
     2521 ggctgccgcg gcgacctggg caaggtagta cgacgaaccc gcgccggtga tcacgatccg
     2581 ctcagccggg gcgagaatcg catcggccgc cgcctgctca ggaccacggg cgtcgagcgc
     2641 ggccgcgatc gcctcaccgg aggtggagct ctcgcgccag gtgacgaggt tctcgaaggc
     2701 attcatcggt cgcgcagctc cacggaatag cgatagcggt cgccgcggac cgtgctccga
     2761 cagaactcga tcgggaggtc gtcctggtcg aaggcgaccc gctcgacccg gagcgccggc
     2821 tcgccacgtc gctggtcgag gagggccgct tcatccgcgg tcagcaggac cggcgagaac
     2881 gtctcccggg cacgggtcgg gcgacagcca taggcgttga gcaatgtgtc gtagagccgg
     2941 acggtcgaga agtcgacggt ctcgaggcgc ggaaaccgcg cggccggcag gtggctcgtc
     3001 tcgacgacca acgggtcggg accggccagc cgcaaccggc gcagggcgac caccctggtg
     3061 ccgccggcca ctccgagggc ttcggcgaca tcgtcggccg cgggcatggt tcggaggtcc
     3121 aggaccctgg tccccggctg aagcccgtgc cgctcgatct cccgactgaa ggaatagaac
     3181 ccaccgatcg tgtgctcgaa ccgcggcgcg gtgacatagg tcccgaggcc gcgccggatc
     3241 tccagcaggc cctccgcgac aagcccggcg agcgcctgcc ggacggtcgc ccggctgacg
     3301 ccaaactccg ccatcagctc tggctccggt gcgacctggg cgcccggctg caggccgccg
     3361 tcgcggatcc gcgcgcgcac gtccgcttcg atccgccggt agaggggcag cccgggggcg
     3421 acggccgggc tcatcccttg atcccggtca gggcgatccc gcggacgaag tgccgctggg
     3481 ccacgatgaa gaggacgatc atcggcagga cgctgacgag cgcgccggcc atcaggagcg
     3541 gccacctccc cacgtactgg ccctggaaca gcgacaggcc ggcggtcacg gtgagcgaat
     3601 cgacgtcccg caggtagatg atcgggtcga tcagatcgtt ccaggaccac atgaacgtga
     3661 agatcgccag cgtggccagc gcgggccggg ccagcggcag gtagatgtgc cggtagatct
     3721 gccacgggtt cgcgccgtcg acccgcgccg cgtcgccgag ctcgcgtggg atcgcgagga
     3781 agaactggcg gaggaggaac gtcccgaagg cccctccgag gaacgccccg acccacagcg
     3841 gctcctgcgt cccgatccag ttgccgctcg agctgaacgg gtgggggagg agccgataga
     3901 ggatgaagtt cgggaccagg acgacctgga acgggatgat cagggtcacg acgaggagcg
     3961 cgaacagggg ctcccggaag cggaaccgga ggacggcgaa cgcgtaggcg gccatcgagc
     4021 agctgatcag ctggccgacc gtgttgagcg ccgcaagctt gaacgagttc acgaagaacg
     4081 tggcgaatgg cagggcgttc cacatgtccg gatagttgtg ccattggggc acgatcggga
     4141 acagcgtcgg cggcgtcgcg aagacctccg cccgggtctt gagcgaggtc gagatcatcc
     4201 acaggaacgg cgccatcatc aggaggccgc caccgatcag caagccgacg aagatcgccc
     4261 gacgggccgt ccggaggcgc cgtccgcgac gggcgggcgc gaccggccgg ggaacggcgc
     4321 cggcggtcgc gctcactggt agtggaccca gcgcttctgc gaccagaagt agatcgccgt
     4381 gaacactgcc acgatcagga acaggatcca cgacgccgcg ctggcgtagc ccatcttgaa
     4441 gaacttgaag ccgttctcgt agatgaaata gacgacggtg atcgtcgcct cggtcgggcg
     4501 tggcttgatg agcacgaaga tctggtcgaa cacctggaag ctgccgatca ggctgaggac
     4561 gccggtgaag aagatcgacg gcgtcatcag cggcagggtc acgcgccgga ggcgcgccca
     4621 gcttccggcg ccgtcgacgc tggcggcgtc gagcaggtcg gacgggatcg cctggaggcc
     4681 ggccaggaag atgatcacgt tcaccccgag cccctgccag atcgacatcg cgacgatcgc
     4741 gggcatggcc agggtcgggt cggacagcca cttctgcgcg tggatgccga ccaggccgag
     4801 gacctcgttc aggaggcccg agtccgcgct gtagatccac tgccagacga gggcgatcgc
     4861 gatcgtcgag gtgacgacgg gcaggaaata ggcggtccgg atgaaggcga tcccgcggat
     4921 cttcgcattg agcgcgattg cgagcccgaa cgcgatcgtc agtccgaccg ggaccgacac
     4981 ggccgtgtag aagaacgtgt tccggagcgc cttcaggaac cggtcgtcgc ccagcagcgc
     5041 ggcatagttg tcgaccccgg tcagcttcgg gacggtcagg aggtcccact tcgtcaggct
     5101 gatcgcgagc gtcgcgagga tcggcccggc cgacaggacg gccaagccga tcagggtcgg
     5161 cgccaggaag atcagcgccc agcgcccctc gcccgccagt ggcccgccgc cccgccgtgg
     5221 ggcggggcgg ctccgggggg tcatctcggc gttcgggtgt gtcgggccgg cctcactggc
     5281 tcgcgaggat ctggtcgagg ctcgggacga cgtcggccag ggcctgcgca gcggtcttct
     5341 tcggctcggt gaagacgttg gcgtcgatct ccgtctggag cttggtcgtc cagtcgttga
     5401 agcccttgaa cgacggcttg aggtgggcgt agtcgatcgc cgcggcgagg acgtcggccc
     5461 cgtcgaacga ggtcgcgaac ggtccggcga ggacttcctt gttggcgggc agcgaggcct
     5521 tgagctgcat cagctgggtc tgcgcggccg gactggccag gtacttcacg aacgcccagg
     5581 cggcgtccgg gttcttcgtg cccttgtaga ccaccgcgcc ggtcgggttg atcgaggtcg
     5641 cctggccggc cggacccttg ggcagcgggg cgatcccgaa gtcgagaccg gctgcgatgt
     5701 gggtggcgac gagccacgag ccgttggcct ccatggccgc ctggccctgc tcgaaggcgt
     5761 cgccgagcgc gtcggtgatc gacgcgtcgg gcatgacctt gtccttccag atcaggtcct
     5821 ggaggaactg gagcccgccc gccgcctcgg cgctgccgac gaggctcgtc ttgtgatcgg
     5881 cggagatgat gtc
//
LOCUS       DATAIW010000310         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_216971, whole genome shotgun sequence.
ACCESSION   DATAIW010000310 DATAIW010000000
VERSION     DATAIW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216971"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VHM48_09625"
     CDS             complement(<1..1014)
                     /locus_tag="VHM48_09625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2755712.1"
                     /translation="MCRTGLVANDAADDGGRLVTVVTSDLKGSTALGEQLDPESLREV
                     LNRYFAVMRVVFESHGGSIEKIIGDAIVAVFGLPFRHEDDPVRAVEAAAESQRALATL
                     NDELDKLWGVRLVNRTGVATGIVHFGRDAEGQHVLIGETMDTSTAMEQNAPPVEILMA
                     ESTYREVRELVEAVDMGLKSPKGSDLQIQSYQLISVLSRAAEEDAAPLEPSVGMRACQ
                     VCGEESPERYRYCVICGVSTATVVARDSRKTVTIVFANPRLHTETGDPLEPKAFTAVM
                     TRYFEAMKAALERHGGTVEKFIGDAVMAVFGLPVRHEDDALRAVRAAADMQAALPALN
                     KGFG"
     gene            1133..1840
                     /locus_tag="VHM48_09630"
     CDS             1133..1840
                     /locus_tag="VHM48_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2755713.1"
                     /translation="MDGIPDGTIGFQGLRACRLFDGVEDNQLRALAQSMRSHHYRDGE
                     TVFHQGDPADALHVVAEGAIMIVLPPAGEGEPAILATLGPGEFFGTLALLDDDPRSAT
                     AVALGKAQTLTLRRDAFLDLIDEDRTLRRALLASLSREIRVITEHVGDLHFLDLPARL
                     VRRILREADGFPRSTSGSVSVPWPYTQAELAGMIGGSRQSVNRLLADLASEGLIRLER
                     DVLTIPDPLALERSIRR"
     gene            1837..2640
                     /locus_tag="VHM48_09635"
     CDS             1837..2640
                     /locus_tag="VHM48_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2755714.1"
                     /translation="MSDGTDARRETATLALLAAVARRAGVAERLESAAGDAVLRSVVD
                     AAVALIRAEAASIALHDPTTDRLVFRVAAGDKGQGVVGLSIGPDEGVAGYVLATGQPL
                     AIAEAASDPRFGRAAAEQTGYMPRSLLAVPLADDAGTIGVLEVLDRRDGAPFDLRDMD
                     AASVFARMATVAIRATRLERDTAHLLELSLRSLDGDLAGDDIDTLVTAAVADLFDDDA
                     TATWRLADLVARVRVADPGQVDLVEEILEALVRRAEHSAPGRYRSVGRL"
     gene            2637..3455
                     /locus_tag="VHM48_09640"
     CDS             2637..3455
                     /locus_tag="VHM48_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2755715.1"
                     /translation="MTTERLPAWSEPFATENRAGLARRRPFGTMERSAVFDRVDGTGI
                     TVAIIDSGVDPDHPAIGGRLVEAVRIELGGDEPRVVPDVETDVVGHGTATAGIIHGIA
                     PGADLISVRVLGPDNRGKGLAFLAALDWAIVRGASVVNLSLSSKSEPLFADFHALADR
                     AYFANVLLVSAANNVPGPSYPSLFAAVVSVAAHDVRDPDVWFYNPAPPVEFGAHGLDV
                     DVAWRGGTRIRATGNSFAAPHLAGRAALIRAAYPDASPFEVKTILAATADDPRT"
     gene            complement(3468..3674)
                     /locus_tag="VHM48_09645"
     CDS             complement(3468..3674)
                     /locus_tag="VHM48_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755716.1"
                     /translation="MTNKRIQLPKDGLIGPGEQLTDDGGEDVEGHGFANPAPPADFSK
                     RSPSTGGDFAPTGEDDDVEGRRVR"
     gene            complement(3777..>4449)
                     /locus_tag="VHM48_09650"
     CDS             complement(3777..>4449)
                     /locus_tag="VHM48_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2755717.1"
                     /translation="IRVLIVVASSLLFVSAAFTLVAPGLGVSTWGAAAARLRGAEAAP
                     AMAVGSGRAATLADFERLAVRLGVFARLSPEQRDSFVRDATIREVPAGTRIVEHGEAA
                     TAAWFILDGSTTAGVPVEDGYRGLSTMTAGDFFGEIGALTGSTRTADVVADVDTTLIE
                     VPAAALRATMAVPEIQRVVLSTMTNRLQKTESADLPRLAGIDQADLRDLRTARPPADV
                     SNPVG"
BASE COUNT          616 a         1599 c         1544 g          690 t
ORIGIN      
        1 gccgaacccc ttgttgagcg ccggcagggc ggcctgcatg tcggccgccg cgcggaccgc
       61 gcggagcgcg tcgtcctcgt gtcggacggg cagcccgaag acggccatga ccgcgtcgcc
      121 gatgaacttc tcgaccgtcc cgccgtgccg ctcgagggcc gccttcatcg cctcgaagta
      181 gcgggtcatg acggcggtga atgccttcgg ctccagcggg tcgccagtct cggtatggag
      241 cctcgggttg gcgaagacga tcgtcacggt cttgcggctg tcgcgcgcga cgacggtcgc
      301 tgtggagacc ccgcagatga cgcaatagcg atagcgttcc ggagattcct cgccgcagac
      361 ctggcacgct cgcatcccga cggacggctc aagcggcgcg gcgtcctcct cggccgcccg
      421 gctcaggacg gagatgagct ggtacgactg gatctggagg tcggagccct tcggcgactt
      481 gaggcccatg tccacggcct ccacgagttc gcggacctcg cgatacgtcg actcggccat
      541 gaggatctcg accggcggcg cgttctgctc catggctgtc gacgtgtcca tcgtctcgcc
      601 gatgaggacg tgctggccct cggcgtcgcg cccgaaatgg acgatgccgg tcgcgacccc
      661 ggtccggttg acgagccgga cgccccacag cttgtcgagt tcatcgttga gggtggcgag
      721 ggcacgctgg ctctcggccg cggcctcgac cgcgcggacc gggtcgtcct cgtgcctgaa
      781 cggcaggccg aagacggcga cgatcgcgtc gccgatgatc ttctcgatgc ttccgccgtg
      841 cgactcgaag acgacccgca tgaccgcgaa atagcggttg agcacctcgc gcagcgactc
      901 cgggtcgagc tgctcgccga gggcggtcga acccttgagg tcagacgtga cgacggtgac
      961 gagcctgccg ccatcgtcgg ccgcgtcgtt ggcgacgagc ccggtacggc acatcccgca
     1021 cagtcgcgcg cccgcgggat tcgcctcacc gcatcgcggg cagaccggct gatccatcgc
     1081 ccaagtctag ccgcggtacg gcgccgcccg gcagtcggct agcatgcgtg cgatggatgg
     1141 catcccggac gggacgatcg gtttccaggg cctccgcgcc tgtcgcctgt tcgacggtgt
     1201 cgaggacaac caactccgcg cgctcgcgca gtcgatgcgc tcgcaccact atcgggacgg
     1261 cgagacggtc ttccaccagg gcgaccccgc cgacgcgctc catgtcgtcg cggagggcgc
     1321 gatcatgatc gtgctgccac cagcgggtga aggggagccg gcgatcctcg caacgctcgg
     1381 accgggcgag ttcttcggga cgctcgccct gctcgatgat gaccctcggt cggcgacggc
     1441 cgtcgcgctc gggaaggcgc agacgctcac cctgcgtcgg gacgcgttcc tcgatctcat
     1501 cgacgaggat cggacgcttc gtcgggcgct cctcgcatcg ctgtcgcgcg agattcgcgt
     1561 gatcaccgag catgtgggcg atctgcattt cctcgatctg cctgcccgtc tcgtccgacg
     1621 aatcctccgc gaggcggatg ggttcccgcg cagcacctcc ggttcggtca gcgtgccctg
     1681 gccatacacc caggctgagc tcgccgggat gatcggcggt tcgcgccaat cggtgaaccg
     1741 cctgctcgcg gatctcgcgt ccgaggggct gatccggctc gagcgcgatg tgctcaccat
     1801 ccccgacccg ctggcgctcg agcggtcgat ccggcggtga gcgacgggac cgacgcgcga
     1861 cgcgagaccg cgacgctcgc gctgctcgcc gcggtcgccc ggcgcgccgg cgtcgcggaa
     1921 cgactggaat cggccgccgg cgacgcagtg ctgcgatcgg tcgtcgacgc cgcggtcgca
     1981 ctcatccgcg ccgaagccgc ctcgatcgcc ctgcacgacc cgacgaccga ccggctcgtc
     2041 ttccgcgtcg cggccggtga caagggccag ggcgtcgtcg gcctgtcgat cgggccggat
     2101 gagggggtcg cgggctacgt tctcgccact ggccagccgc tcgcgatcgc cgaggccgcg
     2161 tccgaccctc gtttcggtcg ggcggcggcc gagcagacgg ggtacatgcc gcgctcgctc
     2221 ctggccgtcc cgctcgctga cgatgccggg acgatcggcg tcctcgaggt gctcgatcgc
     2281 cgcgacggag cccccttcga cctgcgcgac atggatgcgg cgtcggtctt cgcccgcatg
     2341 gcgacggtgg ccatccgggc gacccggctg gagcgagaca cggcccacct cctcgagctg
     2401 tcgctgcgtt ccctcgacgg cgatctcgct ggggacgaca tcgacacgct cgttacggcg
     2461 gccgtcgccg atctgttcga cgatgacgcg acggctacgt ggcgccttgc cgacctcgtt
     2521 gcgcgcgtcc gcgtggccga cccgggccag gtcgacctgg tcgaggagat ccttgaagcg
     2581 ctggtgcgtc gggccgagca ctcggcgccc ggccgctacc gttcagtcgg gcggctgtga
     2641 ccaccgagcg gctgccggcg tggagcgaac cgttcgcaac cgagaaccgg gccgggctgg
     2701 cccgccgccg tccgttcggg acgatggaac gcagcgccgt gttcgacagg gtggacggta
     2761 ccgggatcac ggtcgccatc atcgattccg gcgtggatcc cgaccacccc gcgatcggcg
     2821 ggaggctcgt cgaggcggtc cgcatcgagc tcggcggcga tgaaccccgg gtcgtgccgg
     2881 acgttgagac ggacgtcgtg ggccacggca cggcgacggc ggggatcatc cacggcatcg
     2941 cacccggcgc cgacctgatc tcggtccgcg tgctcggtcc ggacaaccgc ggcaagggac
     3001 tggcgttcct cgctgcgctc gactgggcga tcgtgcgcgg cgccagcgtc gtcaacctga
     3061 gcctgtcctc gaaaagcgaa ccgctctttg ccgacttcca cgcccttgcc gaccgcgcct
     3121 atttcgccaa cgtcctgctc gtctcggctg ccaacaacgt gcccggtccg tcgtatccgt
     3181 cgctcttcgc ggcggtcgtc tccgtggcgg cccacgacgt gcgtgatccg gacgtctggt
     3241 tctacaaccc ggcgccgccg gtcgaattcg gcgcgcacgg cctcgatgtc gatgtcgcct
     3301 ggcgaggcgg gacgcgcatc cgggccacgg gcaactcgtt cgcggcgccc catctcgccg
     3361 gccgtgcggc gctgatcagg gccgcgtatc ccgacgccag cccgttcgag gtcaagacga
     3421 tcctcgccgc cacggcggac gacccccgga catgacggcg gtcaggctca gcgaacgcga
     3481 cggccctcga cgtcgtcatc ctcgccagtc ggggcgaagt cgccaccggt gctcgggctc
     3541 cgcttcgaga aatcggccgg cggggccggg ttcgcgaatc cgtgaccctc gacgtcttcg
     3601 ccgccgtcat cggtcaactg ctcgccgggt ccgatgagcc cgtccttcgg aagctggatc
     3661 cgcttgttcg tcatggccct catcccttcc tgccgtggat atcacggccc tgatgcgggt
     3721 accgtagcgc atccccgagg tgcgctcgat accgggagcg acgacgggac tgtcagctag
     3781 ccgacagggt tcgacacgtc ggccggcggt cgcgccgtgc gcaggtcgcg caggtcggcc
     3841 tggtcgatgc cggccaaccg cggcaggtcc gccgattccg tcttctggag ccggttcgtc
     3901 atggtcgaga ggaccacgcg ctggatctcc ggtacggcca tggtcgcccg gagggccgcc
     3961 gccggcacct cgatcagggt cgtgtcgacg tccgcgacga cgtcagcggt ccgggtgctg
     4021 ccggtgaggg caccgatctc gccgaagaag tccccggcgg tcatcgtcga aagcccacgg
     4081 tagccgtcct cgaccgggac accggcggtc gtcgacccat cgaggatgaa ccaggccgcc
     4141 gtcgccgcct cgccgtgctc cacgatgcgt gtcccggccg ggacctcgcg gatcgtggcg
     4201 tcgcggacga acgagtcacg ctgctcgggt gatagtctgg cgaacacgcc gagacggacc
     4261 gcgaggcgtt cgaagtcggc gagcgttgcc gcccggcccg acccgaccgc catcgccggg
     4321 gccgcctccg ccccgcgcaa ccgagccgcg gcggcccccc acgtcgacac gcccaggccg
     4381 ggtgcgacga gcgtgaacgc ggccgacacg aacaggagcg aggaggcgac gacgatcagc
     4441 acccggata
//
LOCUS       DATAIW010000311         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_19281, whole genome shotgun sequence.
ACCESSION   DATAIW010000311 DATAIW010000000
VERSION     DATAIW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19281"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VHM48_09655"
     CDS             complement(<1..414)
                     /locus_tag="VHM48_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX2755718.1"
                     /translation="MTDDTESQPIAMDFEGAGDGRERALAMLLLSMADDEFVIGFSDS
                     EWTGIGPILEEDVAISSLAQDELGHAQALYGLLADAVADGRDADAWAYDRPPEGYLHA
                     RLLDHPRGDWAATIVRRYLYDTADGARLAALAASSD"
     gene            complement(411..755)
                     /locus_tag="VHM48_09660"
     CDS             complement(411..755)
                     /locus_tag="VHM48_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755719.1"
                     /translation="MAEGSGAPASVLEPWEVFRREKDGDPMRHGGSVMAPDATLALHY
                     AREMFSRRQESVQLWVVRRSDIHDLADLDLLQPPLDRSFKKPGGYVMRDKLAAARKQS
                     GQAKPKANKGLR"
     gene            complement(782..1777)
                     /gene="paaA"
                     /locus_tag="VHM48_09665"
     CDS             complement(782..1777)
                     /gene="paaA"
                     /locus_tag="VHM48_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX2755720.1"
                     /translation="MTDPLALDPVLPPRERSALPFNDDALDHDERYRHFEEHVTGGGK
                     VEATDWMPDEYRTAVLRFVEMHANSELMGVLPEREWIMRAPTLRRKLALTAKVQDEVG
                     HAQLLYRVAEDLGKPREAMIQDLLDGKTKFHNVFHYPTKSWADIGIIAWLVDAAAIVA
                     QQALRDSSYAPYARTMKKICWEESVHIMHGRDVVVTMATGTPTQRAAIQEALDRWWGP
                     LMQMHGPRSDRAKDRDIHWKIKAKTSEELRQEFIGIYVPRIRELGLTIPDAALALDPA
                     TGAWAYGEPDWNELRTVVTNHGPMSQERLDFRRANWDATQWVRDAILGNPRAAVA"
     gene            complement(1774..2364)
                     /locus_tag="VHM48_09670"
     CDS             complement(1774..2364)
                     /locus_tag="VHM48_09670"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2755721.1"
                     /translation="MIETGARRREIDEVASELFRANGYAATSIRDIARALDMQGASLY
                     AHVASKEDLLWAIVDRAASSFEMAADRAADETSGADAVERLAALVEAHVEVVLADPGR
                     TSVFVTEWRHLSGGRRAAIAGRRDAYEARFRETIADGISIGAFGATDAAIAAAFILTA
                     LNGIATWYRPEGRLTSDRIADHIVELALRSLSEDHR"
     gene            complement(2765..>2951)
                     /locus_tag="VHM48_09675"
     CDS             complement(2765..>2951)
                     /locus_tag="VHM48_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755722.1"
                     /translation="DFIVQHDGARQVVRPPGYGGGQGTLSPDRQSLIYQRSETARLTV
                     LNLQSGQIEGELSSLEF"
BASE COUNT          419 a         1003 c         1049 g          480 t
ORIGIN      
        1 atcggaggac gcggcgaggg ccgctaacct ggcgccgtcg gcggtgtcgt agaggtagcg
       61 gcggacgatc gtcgccgccc agtcgccccg cggatggtcg agcagccgcg cgtggaggta
      121 gccctcgggc ggtcggtcgt aggcccatgc gtccgcgtcc cgaccgtccg cgaccgcgtc
      181 ggcgagcagc ccgtacagcg cctgggcgtg accgagctcg tcctgggcga gcgagctgat
      241 cgcgacgtcc tcctcgagga tgggcccgat cccggtccac tcggagtcgg agaacccgat
      301 gacgaattcg tcgtcggcca tcgagagcag gagcatcgcg agcgcgcgct cccgcccgtc
      361 gccggcgccc tcgaagtcca tcgcgatcgg ctgggactcc gtgtcgtcgg tcatcgcagg
      421 cccttgttgg ccttcggctt cgcttgcccc gactgtttgc gagcggcggc cagcttgtcg
      481 cgcatcacgt agccgcccgg cttcttgaag gagcggtcga gcggcggctg gagcaggtcg
      541 aggtcggcga ggtcgtggat gtccgaccgc cggacgaccc acaactggac gctctcctgg
      601 cgccggctga acatctcgcg ggcgtagtgg agggcgagcg tcgcgtcggg cgccatcacc
      661 gaaccgccgt gccgcatcgg gtcgccgtcc ttctcgcgcc ggaagacttc ccatggttcg
      721 aggacgctgg cgggcgcgcc ggagccctcg gccacggcgc gatcgggcgg tgccgaatcc
      781 gtcacgccac ggccgcccga ggattgccga ggatcgcgtc gcggacccac tgcgtcgcgt
      841 cccagttcgc gcggcggaag tcgagccgct cctgcgacat cgggccgtga ttggtgacga
      901 cggtccggag ctcgttccag tcgggctcgc cataagccca ggcgccggtt gccgggtcga
      961 gggcgagagc ggcgtccggg atcgtcaggc cgagctcccg gatccgcggc acgtagatcc
     1021 cgatgaactc ctggcggagc tcctcgctcg tcttcgcctt gatcttccag tggatgtcgc
     1081 ggtccttggc ccggtcgctg cgcggcccgt gcatctgcat gagcggaccc caccagcgat
     1141 cgagcgcctc ctggatcgcc gcgcgctggg tgggcgtccc ggtcgccatc gtcaccacga
     1201 cgtcgcggcc gtgcatgatg tggactgact cctcccagca gatcttcttc atggtccggg
     1261 cgtacggagc gtaggaagag tcccgcagcg cctgttgggc gacgatcgcg gccgcgtcga
     1321 cgagccaggc gatgatcccg atgtcggccc acgatttcgt cgggtagtgg aagacgttgt
     1381 gaaacttcgt cttgccgtcg aggagatcct ggatcatcgc ctcgcgcggc ttcccgagat
     1441 cctcggccac gcgataaagc agctgggcgt ggccgacctc gtcctggacc ttggcggtga
     1501 gggccagctt ccggcggagg gtcggcgccc gcatgatcca ctcgcgctcg ggcaggacgc
     1561 ccatcagctc gctgttggcg tgcatctcga cgaagcgcag gaccgcggtc cggtactcgt
     1621 cgggcatcca gtcggttgcc tcgaccttgc cgccgccggt gacgtgctcc tcgaagtggc
     1681 ggtagcgctc gtcgtggtcg agggcgtcgt cgttgaacgg cagcgcgctc cgctcgcgcg
     1741 gcggtaggac ggggtcgagg gcgagcgggt cggtcatcgg tggtcctccg agagggagcg
     1801 cagggcgagc tcgacgatgt ggtcggcgat ccggtcagag gtgaggcgac cttccggccg
     1861 gtaccaggtc gcgatcccgt tgagggcggt caggatgaac gcggcggcga tggcagcgtc
     1921 ggtcgcgccg aaggccccga tcgagatccc gtcggcgatc gtctcgcgga accgcgcctc
     1981 gtacgcgtca cggcgcccgg cgatcgcggc gcgccggccg ccggacaggt gacgccattc
     2041 cgtcacgaac acgctcgtcc gccccggatc ggcgaggacg acctcgacgt gagcctcgac
     2101 gagggcggcg agccgctcga cggcgtccgc cccgctggtc tcgtcggccg cccgatccgc
     2161 cgccatctcg aacgacgagg ccgcccggtc gacgatcgcc cagaggaggt cctccttgga
     2221 cgcgacgtgg gcgtagaggc tggccccctg catgtcgagc gcccgggcga tatcgcggat
     2281 gctcgtcgcc gcgtagccgt tcgcgcggaa cagctcgctc gcaacctcgt cgatctcgcg
     2341 tcggcgcgct ccggtctcga tcacgtgtcg gctcctacct aacgatcgtt aggattgtcg
     2401 ctccggaccg atgtgccgtc aaccgttcgg acgcgactga cgcggtgccc agatctccgg
     2461 cagctggagc ggcagacgca gcgatgagcg cgcaacgacg accacagaaa gcccccacgc
     2521 cgtcttggac tgaatcgcca tgaacgcgac ggccggtagg gcgagcaggc cgccaggcga
     2581 tgtcagatgc agcctgtccc ggcgaccgcg actggccgag tcttccatag atcgttcgtg
     2641 cggcggtccg tccggccgga tgtcacaaca gagcggcgac gtccgagagg cagagcttgt
     2701 tcgactctcc agggccatgt gcggtgaagg cggcgaggat cccggctgac gagacctcgc
     2761 cgccttagaa ctccaacgag ctgagttcgc cctcgatttg acccgactgc aggttgagta
     2821 cggtcagcct cgcggtctcg ctccgttggt agatcagcga ttgccgatct gggctgaggg
     2881 tcccctgtcc gccgccgtag ccgggcggtc gaacgacctg gcgcgcaccg tcatgctgaa
     2941 cgatgaagtc c
//
LOCUS       DATAIW010000312         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_19289, whole genome shotgun sequence.
ACCESSION   DATAIW010000312 DATAIW010000000
VERSION     DATAIW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19289"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VHM48_09680"
     CDS             complement(<1..821)
                     /locus_tag="VHM48_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755723.1"
                     /translation="MARPAIAIALPGDEQPHVVDVLKEADFEPVVVGCPGDLATLLAS
                     RHDVAVAILDGESDFDESLEYYELLHEPGRDIPALMIVSPRTLDRLAGAAGRAGAHDE
                     YFTRPYSPEALRWRVEAMLIRQMTVDDGSGAVIQSGSLSAEDWSRRGAFLSVFNPKGG
                     VGKTTIAVNLAASLVAMGRKVLLVDADTVTGHIASSLGLENVRTLVDSLAETHESGPG
                     AVPETLEELVAAHSSGLKVLVLSSSPLKTGHLDPAAVADAIDRARRSFDVIVVDL"
     gene            complement(857..>1813)
                     /locus_tag="VHM48_09685"
     CDS             complement(857..>1813)
                     /locus_tag="VHM48_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755724.1"
                     /translation="VTRIQGQIASFEAEMAAFFDELLTETDLTILAARAQQMPEAPAF
                     DEMDDTAFTALMTEPAVGIPIAEPEPIAEPEPIAEPEPIAEMPTTALEAEEVTVADTA
                     PAATPSPTGDLSFDRDAAMAAIQTAAEAAASVEAAPAEPGLEAAADASVAEPDVALPS
                     APEDNTPARDPRLAMLGLTPDFAAAEAAASEAAAAEISEIPELDEENLAARLAGLVPS
                     STSGAPADAPPAAPVSSTQVIVTGLVSVASIASFKRHLSRQAGVRHVGVSSGPDGEFL
                     FTVQHQAEVALRDLIPTLPGFQARVTGGGDGIVTASAHDPED"
BASE COUNT          233 a          617 c          698 g          265 t
ORIGIN      
        1 tggagatcga cgacgatgac gtcgaaggag cggcgcgcgc ggtcgatcgc gtcggcgacc
       61 gccgccgggt cgaggtggcc ggtcttcagc gggctggacg agaggacgag caccttcagg
      121 ccggacgagt gcgcggcgac cagctcctcc agcgtctcgg ggacggcgcc cggcccgctc
      181 tcgtgggttt cggcgagcga atcgacgagg gttcggacgt tctccaggcc gagcgagctc
      241 gcgatgtggc cggtcacggt gtcagcgtcc acgagcagga ctttccggcc catcgcgacg
      301 agcgaggccg ccaggttgac ggcgatcgtc gtcttgccca ccccgccctt cgggttgaag
      361 acgctcagga acgcgccccg ccgcgaccag tcttcggccg agagcgagcc ggactggatc
      421 accgcgccgc tgccgtcgtc gacggtcatc tggcggatca gcatcgcctc cacgcgccag
      481 cggagcgcct ccggcgagta gggtcgggtg aagtactcgt cgtgcgcgcc ggcgcgcccg
      541 gccgcaccgg ccagccggtc gagcgtgcgc ggtgagacga tcatgagggc cgggatgtcc
      601 cgccccggct cgtggaggag ctcgtagtac tcgagcgatt cgtcgaagtc gctctcgccg
      661 tcgaggatcg caacggcgac gtcgtggcgg ctcgcgagga gcgtcgcgag gtcgcccggg
      721 cacccgacga cgaccggctc gaagtcagct tccttgagca cgtcgaccac gtgcggctgt
      781 tcgtcgccgg gcagtgcgat cgcgatcgcg gggcgggcca tccctccggg tcctttcgtc
      841 tcgcggactg gtgtgctcag tcctcggggt cgtgggcgct cgcggtcacg atcccgtcac
      901 cgccgccggt gacccgggcc tggaagccgg gaagcgtcgg gatcaggtcg cggagggcga
      961 cctccgcctg gtgctggacg gtgaacagga actcgccgtc cggcccggac gagacgccca
     1021 cgtggcgcac gcccgcctgg cggctgagat ggcgcttgaa gctcgcgatg ctcgcgacgc
     1081 tgacgaggcc cgtcacgatg acctgggtcg aggacacggg cgcggctggg ggcgcgtcgg
     1141 ctggggcgcc ggacgtcgac gacgggacga ggccggcgag gcgggcggcg aggttctcct
     1201 cgtcgagctc cgggatctcc gagatctcgg ccgcggcggc ttccgaggcg gcggcctcgg
     1261 ccgcggcgaa gtcgggagtc agcccgagca tcgcgaggcg cggatcacgg gccggggtgt
     1321 tgtcctccgg tgccgacggc agcgcgacat cgggctcggc cacggacgcg tcggcggccg
     1381 cctcgagccc gggctcggca ggcgcagcct cgaccgacgc cgcggcctcg gcggccgtct
     1441 ggatcgcggc catcgcggcg tcgcggtcga acgagaggtc gccggtcggg gatggcgttg
     1501 cggccggcgc ggtgtcggcg accgtcacct cctcggcctc cagggccgtc gtcggcatct
     1561 cggcgatcgg ctccggttcg gcgatcggct ccggttcggc gatcggctcc ggctcggcga
     1621 tcgggatccc gacggcaggc tcggtcatga gcgcggtgaa cgcggtgtcg tccatctcgt
     1681 cgaaggccgg cgcttcgggc atctgctgag cgcgtgcagc caggatcgtc aggtccgtct
     1741 ccgtgagcag ctcatcgaag aaggccgcca tctccgcctc gaacgaggcg atctgtccct
     1801 ggatccgggt gac
//
LOCUS       DATAIW010000313         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_246811, whole genome shotgun sequence.
ACCESSION   DATAIW010000313 DATAIW010000000
VERSION     DATAIW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246811"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VHM48_09690"
     CDS             complement(<1..245)
                     /locus_tag="VHM48_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2755725.1"
                     /translation="MSERATSDEAVRDLEIRLFGPMRVLVGGQEVDPGARRQRGVLAI
                     LALAAGEVVSVDHIIDRLWGEHAPAAAKNTIQVYIGGL"
     gene            403..1608
                     /locus_tag="VHM48_09695"
     CDS             403..1608
                     /locus_tag="VHM48_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755726.1"
                     /translation="MDPVLDRIDDWERAGLIDGSTAARLRAAEAGRADAAPSPDLAGE
                     RTPIDPVLPQSRAGHLSVSAVFGPGVTIGEMFGYLGGGFILGAWSAFAVRLAGSNVDT
                     SMIGAGALIAAVGLVVVGLALGTADPRRRRAAGVALLVAVPYVSIAAAAFLGRSRLDP
                     YLLAIVVTAVTVGAATLTRLLLPALLTTLGLVGSITGFGWAVLAYALTLVTPRFDVGF
                     PSQPPQNEVPVVIVSAVGWLLVALVLGFFAIAEDRAAPEPADDPDGHDAARRRSTLIR
                     AWAGLVAVWGLASAVSRSGYGFDFGRVIPAWIGDAAILVLALILVERAFRRDSGAFLF
                     AAGIGFITALTDFNFTYLGESRDLGLLIEGGILLAVGFAADRLRRRMPGGRSMPPPSV
                     VEPVANTAS"
     gene            complement(1631..2479)
                     /locus_tag="VHM48_09700"
     CDS             complement(1631..2479)
                     /locus_tag="VHM48_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX2755727.1"
                     /translation="MDDQRARLEDRRTADRARFARLVARPEPDIDLAIGALLIAGLGR
                     PAIDEETILGQLDAIAERVRIRLDSGDPTPAVVARLHDVLYRELGFRGPTAAEYGDPP
                     NSHIDEVIARRIGLPISLAIVELEVAARLGLALVGIGLPGHFIVGGPDGTLFDPADGG
                     RALTPDDCQALIRRAIGDGVLLHAGMLRPAGRREILARVLRNLRTARLGRRDWPAAVD
                     AIDLLLVLEPTDPEHGRDRGLLLGRMGRFGEAIAALRLYLDERPDGNDVADVRQVMGI
                     FAGRRN"
     gene            2555..2836
                     /locus_tag="VHM48_09705"
     CDS             2555..2836
                     /locus_tag="VHM48_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755728.1"
                     /translation="MDPTDETRRRPDEDANLALEPARRNRRRRAQGGLGDTIGGVIVG
                     FDYQVFRATKPPAILVESAKPVRGLSGEGGTMLSIDFPGDVPSTDDLAT"
     gene            2922..3386
                     /locus_tag="VHM48_09710"
     CDS             2922..3386
                     /locus_tag="VHM48_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755729.1"
                     /translation="MPADSPSPSIAPGLGPLRPAPAGLPEPTLAPPVVAESADPFTTL
                     RVIVLLSRIERARPVRLADLVDRLNATHLDWLFPVAVVADVAVALQSNWMADYRNGSG
                     IVLADGPYGPTIEIEDSSRVDPWIVRQAEREAAACRERLAAFSRLDRPTGEG"
     gene            3518..>3858
                     /locus_tag="VHM48_09715"
     CDS             3518..>3858
                     /locus_tag="VHM48_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2755730.1"
                     /translation="MTVSGYANDVLVDAAWARAHLDDPSVRFVEVDVDTTAYDQSHLP
                     GAVAWNWTSQLADGIRRDIASRAEFSALLSAGGIGPDTTIVLYGDNNNWFAAWAYWQL
                     KLFGQRDVRIL"
BASE COUNT          505 a         1409 c         1343 g          601 t
ORIGIN      
        1 agtccaccga tgtagacctg gatcgtgttc ttcgcggccg ccggggcgtg ttctccccag
       61 agccggtcga tgatgtgatc gaccgacacg acctcgccgg cagccagcgc gaggatggcg
      121 aggacgcctc gttgccgccg agctccgggg tcgacctcct gcccgccgac caggacgcgc
      181 atcggaccga acagccggat ctcgaggtcg cgaaccgcct catcggacgt cgcgcgttcg
      241 ctcattgagc ggcattgtgc gggtccggcc gcgtccggat tccccgtttc gaccgacgaa
      301 ggaatcgcgt ccaggtgcgg cggctcccga cacggtgcaa cgtccggggc gcaagatcgt
      361 caggtttccg aacgcagggc tccgccgaag gaggggcaag tcatggaccc ggtgctggac
      421 cgcatcgacg attgggaacg agccggcttg atcgatggct cgacagccgc gcggctgcgc
      481 gcggccgagg cgggccgcgc tgacgccgct ccatcgccgg acctggccgg cgagcggacc
      541 ccgatcgatc cggttctgcc gcaatcgagg gccggccatc tctccgtgtc cgcggtgttc
      601 gggccgggcg tcacgatcgg cgagatgttc ggatacctgg gcggcgggtt catcctgggc
      661 gcctggtcgg ccttcgcggt ccggctcgcc gggtcgaacg tcgacacgtc gatgatcgga
      721 gccggcgccc tcattgcggc cgtcggcctg gtcgtcgtcg gcctcgccct cggaaccgcc
      781 gaccctcgcc gtcggcgcgc ggccggggtc gcgctgctcg tggcggtgcc ctacgtttcg
      841 atcgcggccg ccgccttcct cggccgctcg cgtctcgacc cctacctgct cgcgatcgtc
      901 gtgaccgccg tgacggtcgg tgccgcgacc ctcaccaggc tgctcctgcc ggctctcctg
      961 acgacgctcg ggctggtcgg ctcgatcacc ggcttcggtt gggcggtcct cgcgtacgcc
     1021 ctcacgctcg tgacgccacg cttcgacgtc ggcttcccga gccagcctcc acagaacgag
     1081 gtgccggtcg tgatcgtctc ggccgtcggc tggctgctcg tcgccctcgt gctcggcttc
     1141 ttcgccatcg cggaagatcg cgccgccccg gagccggcgg acgatccgga cggtcacgac
     1201 gccgcacgcc gccgatcgac gctgatccgg gcgtgggcgg gtctggtcgc ggtgtggggc
     1261 ctggcatcgg ccgtcagccg atccgggtac ggcttcgact tcggacgggt gatcccggcg
     1321 tggatcggcg acgcggcgat cctggtcctc gcactcatcc tcgtggagcg ggcgttccgg
     1381 cgcgattccg gcgcgttcct gttcgccgcc ggcatcgggt tcatcaccgc gctcaccgac
     1441 ttcaacttca cctacctcgg tgagagtcgc gacctcggcc tgctcatcga gggtgggatc
     1501 ctgctcgccg tcgggttcgc tgccgatcgc ctccgtcgcc ggatgccggg cgggcgatcg
     1561 atgccgccgc cgtccgtggt cgagccggtc gcgaacaccg cctcctgaac cgagccgacc
     1621 gcgctcgcct tcaattccgg cggcccgcga agatgcccat gacctggcgg acgtccgcga
     1681 cgtcgttgcc atcgggtcgt tcgtcgaggt agagccggag cgcggcgatc gcctcaccga
     1741 accggcccat ccggccgagc aggaggccgc gatcgcggcc gtgctccggg tccgtcggct
     1801 cgaggacgag caacaggtcg atcgcgtcga cggcggccgg ccaatcgcgc cgcccgagcc
     1861 gggccgtcct gagattgcgc aggacccggg cgaggatctc gcgccggccg gccggcctga
     1921 gcatgccggc gtggagcagc acgccatccc cgatcgcgcg tcggatcagc gcctggcagt
     1981 cgtcgggggt cagcgcccgg ccgccatcgg ccggatcgaa cagggttccg tcgggaccgc
     2041 cgacgatgaa atgaccgggc agcccgatcc cgacgagggc gagcccgagc cgggcggcga
     2101 cctccagctc gacgatcgca aggctgatcg gcagcccgat ccggcgggcg atgacctcgt
     2161 cgatgtggct gttcggcgga tcgccatact cggccgcggt cggcccgcgg aagccgagct
     2221 cgcggtacag gacgtcgtgg aggcgagcga cgacggcggg cgtcggatcg ccggagtcga
     2281 ggcggatccg gacgcgctcg gcgatggcgt ccagctggcc gaggatcgtt tcctcgtcga
     2341 tggctggacg cccgaggccc gcgatcagga gcgcgccgat cgcgagatcg atgtccggct
     2401 ccggtcgcgc gacgaggcgg gcgaaccggg cgcgatcggc ggtgcgacgg tcctcgagcc
     2461 gcgcacgctg gtcgtccatc tgcggagtct aggacggaag cacccgggcc cgcgtctcgc
     2521 tcgtcgtctg gacaggtggg ctaccgtgca ctgcatggac ccgaccgacg agacgcgccg
     2581 tcgccccgac gaggacgcca acctcgccct cgagcccgcc cgccggaacc ggcgccggcg
     2641 tgcccagggc ggcctcggcg acacgatcgg cggtgtcatc gtcggcttcg actaccaggt
     2701 gttccgggcg accaagccgc cggcgatcct cgtggaatcg gcgaagccgg tgcgcggcct
     2761 gtccggcgag ggcgggacga tgctctcgat cgacttcccg ggggacgtgc cgtccacgga
     2821 cgacctggcg acctgagacc agcgatccgg cgggcgtcgt ggatcgtctt ggggtaggct
     2881 gcgcaattcc tggtggattc ctcggagata acgcctcgcc gatgccagcc gacagcccgt
     2941 caccgtcgat cgcgcccgga ctcggcccgc tccgcccggc gcccgccggc ctccccgaac
     3001 ccacgctggc gcccccggtc gtcgccgaat ccgccgatcc gttcacgacc ctccgggtca
     3061 tcgttctgct ctcccggatc gagcgggcac ggcccgtccg cctggccgat ctcgtcgatc
     3121 ggttgaacgc gacccacctc gactggctct ttccggtcgc ggtcgtcgcg gacgtcgccg
     3181 tcgctctcca gtcgaactgg atggccgact accgcaacgg ctcggggatc gtcctcgccg
     3241 acggaccgta cggcccgacg atcgagatcg aggacagcag ccgggtcgat ccgtggatcg
     3301 tccgccaggc tgagcgcgaa gccgccgcct gtcgcgagcg tctggccgca ttcagccggc
     3361 tcgaccggcc gaccggggag ggatagcccg atgtgcttgt gtccgcggcg ggcccgatcc
     3421 cgctgatcgt cgcccgcgac cctcgccgtt ccgttcatcc gacacgtctc gcggccgcgc
     3481 ccgttcgcct ggtcgccgca ccgaagggag cattccagtg accgtttccg gatacgccaa
     3541 cgatgtcctc gtcgacgcag cctgggctcg tgcccacctc gacgatccgt ccgtccgttt
     3601 cgtcgaggtc gatgtcgata cgaccgccta tgaccagagc catctaccgg gggccgtcgc
     3661 ctggaactgg accagccagc tggccgacgg gatccgccgc gacatcgcga gccgcgcgga
     3721 gttcagcgcc ctgctcagcg ccggcggcat cggcccggac acgacgatcg tcctctacgg
     3781 cgacaacaac aactggttcg ccgcgtgggc gtactggcag ctcaagctgt tcggccagcg
     3841 cgacgtccgg atcctcaa
//
LOCUS       DATAIW010000314         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_168920, whole genome shotgun sequence.
ACCESSION   DATAIW010000314 DATAIW010000000
VERSION     DATAIW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168920"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2218
                     /locus_tag="VHM48_09720"
     CDS             <1..>2218
                     /locus_tag="VHM48_09720"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2755731.1"
                     /translation="PDLSALPPLLAATGTFADLRKRLIAPLSASAGGGANSATNGVGS
                     VAPSRRHGRHVGLSSVPHGAKTFLAATLAGVAEGERLAWIARDAEIGDRVAEELAAWL
                     GDPGGVVVLEPRTALAYERSELVADETAARVAALSTWRSGGARILVASVQALLQHTIT
                     PDDLPAEPRRLRIGGRLRPEALLRELLGLGYSPASEVAGRGEFARRGGIVDVFPPSAP
                     LPVRIEFFGDEIDSLRAFDPTDQRTVGPLDEAILLPASEFLAPHGGAAALRARLGRAA
                     AKLPERLAADLARFEGDGSEATSAADGPVAGTEGLTSRALTVGDAAEVWAAQLAPATG
                     FDHLDPATLLILDEPGEIAEAADFLWRQADERRGELIETGDLPKDWPSTYLGPRDWKT
                     RLVASRTLELTWESEIAEGAALAARGLSSGDPFGWHEPQLPVTRAAGIAAAVERWQAD
                     GARIVLTSDQAPRLAELLDEAGHPVAVVHAPEAPPSGAVAVVERSLNGGFAGGPDGLV
                     FVTDRELFGSVRVRRPKAMRRVVPRDILERLTPGDLVVHIDHGVARYERMLRRGNPAE
                     GEDRDYLELTFAGTDKIFVPVEQITRISRYSGAEHPQLSKLGGTEWLKTKQRVKKAVA
                     DLAEELLALYASRANAPGHAFADDTPWQQEMEASFPYEETPDQLRAVVEVKLDMEAGR
                     PMDRLVVGDVGYGKTEVALRAAFKAIQDGKQVAVLVPTTVLAAQHHQTFSQRFAAFP"
BASE COUNT          293 a          849 c          794 g          282 t
ORIGIN      
        1 agccggacct gtcggccctg ccgccgctgc tggccgcgac cggcacgttc gctgacctcc
       61 gcaagcggct gatcgcgccg ctttcggcat cggcgggggg cggcgcgaac agcgctacga
      121 acggcgtcgg atcggttgcc ccgtcgcggc ggcacggccg tcacgtcggc ctgagctccg
      181 tcccgcacgg cgccaagacg ttcctcgccg cgaccctcgc cggcgtcgcc gagggcgagc
      241 gattggcgtg gatcgcccgc gacgccgaga tcggcgatcg cgtcgccgag gagctggcag
      301 cgtggctcgg cgatccgggc ggggtcgtcg tgctcgagcc gcggaccgcg ctcgcgtacg
      361 agcggagcga gctcgtcgcc gacgagaccg cggcccgcgt cgccgccctg tcgacctggc
      421 ggagcggcgg cgcgcggatc ctcgtcgcga gcgtccaggc cctcctccag cacacgatca
      481 cgccagatga tctcccggcc gaaccgcgcc ggttgcggat cggcggccgg ctccgtccgg
      541 aggcgctcct gcgcgagctc ctcggcctcg gctattcgcc cgcgtcagag gtcgccgggc
      601 ggggcgagtt cgcccggcgc ggcgggatcg tcgacgtctt cccgccgtcg gcgccgctgc
      661 cggtccggat cgagttcttc ggcgacgaga tcgattcgct gcgagcgttc gacccgaccg
      721 accagcgcac cgtcggcccg ctcgacgagg cgatcctgct gcccgcgtcg gagttcctcg
      781 cgccgcacgg gggagcggcc gcgctccgcg cgcggcttgg ccgggctgcc gcgaagctgc
      841 cggagcgact cgccgccgac ctggcccgct tcgagggcga cggctcggaa gcaacgagcg
      901 cggccgatgg tccggtcgcc gggaccgagg gcctcacgag ccgcgccctg accgtcggcg
      961 acgccgcgga agtgtgggcc gcccagctcg ccccggcgac cggcttcgac cacctcgacc
     1021 ccgcgaccct cctgatcctc gacgaacccg gcgagatcgc cgaggccgcc gacttcctgt
     1081 ggcgccaggc tgacgagcgc cgcggcgagc tgatcgagac cggcgacctg ccgaaggact
     1141 ggccatcgac ctacctcgga ccacgcgact ggaagacccg cctggtcgcc agccggaccc
     1201 tcgagctcac gtgggagtcg gagatcgcgg agggcgcggc gctcgccgcc cggggcctca
     1261 gctcgggcga tccgttcgga tggcacgagc cgcagctgcc cgtgacccgg gcggccggga
     1321 tcgccgccgc cgtcgagcgc tggcaggccg atggcgcccg gatcgtcctc accagcgacc
     1381 aggcgccccg cctcgccgag ctgctcgacg aggccggtca tcccgtcgcc gtcgtccacg
     1441 ctccggaggc cccgccgtcc ggcgccgtcg ccgtcgtcga gcgcagcctg aacggcgggt
     1501 tcgcgggcgg ccccgacggg ctcgtgttcg tcaccgaccg ggagctgttc gggagcgtcc
     1561 gtgtccgccg cccgaaggcg atgcgacggg tcgtcccgcg cgacatcctc gaacgcctga
     1621 cgcccggtga cctcgtcgtc cacatcgatc acggtgtcgc ccgctacgag cggatgctcc
     1681 ggcgcggcaa cccggccgag ggcgaggatc gcgactatct cgagctgacc ttcgccggga
     1741 ccgacaagat cttcgtcccc gtcgagcaga tcacccggat ctcgcgctac agcggcgccg
     1801 agcaccccca gctcagcaag ctcggcggga ccgaatggct caagaccaag caacgcgtca
     1861 agaaggcggt cgcggacctg gccgaggagc tcctcgccct gtacgcctcg cgagcgaacg
     1921 cgcccggtca cgccttcgcc gacgacacgc cgtggcagca ggagatggag gcgagcttcc
     1981 cgtacgagga gacgccggac cagctccggg cggtcgtcga ggtcaagctc gacatggagg
     2041 ccggccggcc gatggaccgg ctcgtcgtcg gcgacgtcgg ctacggcaag accgaggtcg
     2101 ccctgcgggc cgcgttcaag gcgatccagg acggcaagca ggtcgcggtc ctcgtcccga
     2161 cgacggtcct cgccgcgcag caccaccaga cgttcagcca gcggttcgcc gcgtttcc
//
LOCUS       DATAIW010000315         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_276758, whole genome shotgun sequence.
ACCESSION   DATAIW010000315 DATAIW010000000
VERSION     DATAIW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276758"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VHM48_09725"
     CDS             <1..180
                     /locus_tag="VHM48_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755732.1"
                     /translation="TSLSARSISASRSRPELVPVLERALGADRDAEIRRALGDVVAIA
                     HDRFRRAAGAMEVSR"
     gene            177..1010
                     /gene="pucL"
                     /locus_tag="VHM48_09730"
     CDS             177..1010
                     /gene="pucL"
                     /locus_tag="VHM48_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX2755733.1"
                     /translation="MIEVGPNSYGKSAIRLVKVVRGETPHRVRDLTIAIALEGDFAAS
                     YRDGDNSAVIATDTMKNTAYALAGEHLTGAIEDFALVLGRHFLDAPQVERVRVTIAEH
                     AWRPIGAAVDAFSRDGSETRTAAVVVGRDGAAVRAGIADLSVMKTTRSAFEGFPRDGF
                     TTLKETADRIMATKVTATWAYEPAAGLDFDASFAGVRSTFLEVMADHHSVSVQGSIWI
                     IGKAILERHPEVAEVSMSLPNLHHWTVDLSPFGIANDRAVYISTTEPHGLIEATIRRG
                     G"
     gene            1301..2563
                     /locus_tag="VHM48_09735"
     CDS             1301..2563
                     /locus_tag="VHM48_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755734.1"
                     /translation="MEARIVQLPTNEIRVDLDRIVIERLIVRDPALAAIVAGRDAEDR
                     PAFVERALKIGLTALQDATVSVDVDLVRGEFDKLLRSSEQANAKAAEALDQVLRSNFA
                     DGDGRLPRTLEKFLGDRGALRSFVGELFDETKRDSAIGRMRTLLGTYFDGDASRLALL
                     LDPTRQHSPMHQFRTEVTAGFDKLHERLTAIEAAAAARGTERARSAAKGADFEDVLEA
                     MLADVARGSGDLVDRTGCETGELLRSKKGDFVVTVDPARTGGADVRVVVEAKDRPMSV
                     RSIREELREAKQNRAAAVGLVVFSPAHAPSGIAPFDVRAGDVYCVIDPADPDPATLEA
                     ALRLARLLAITSLRDVEAEVDVEAVRAALAAVRAELDALKGIKSTLTSISSSASGLQI
                     TLDRLRDGVIARVADAEAEIRAARAAAN"
BASE COUNT          397 a          934 c          974 g          398 t
ORIGIN      
        1 acgtcactga gcgcccgctc gatctcggcg tcgcggtcgc gccctgaact cgtgccggtt
       61 ctcgagcgcg ccctgggcgc cgaccgcgac gccgagatcc ggcgggcgct cggtgacgtc
      121 gtcgcgatcg cccatgatcg gttcaggcgg gcggcgggag cgatggaggt ctcacggtga
      181 tcgaggttgg ccccaacagc tacggcaagt cggcgatccg gctggtcaag gtcgtgcgcg
      241 gcgaaacgcc ccatcgcgtc cgcgacctga cgatcgcgat cgccctcgag ggcgacttcg
      301 cggcctcgta ccgcgacggc gacaacagcg ccgtgatcgc gaccgacacg atgaagaaca
      361 cggcgtatgc cctcgccggc gagcacctca ccggtgcgat cgaggacttc gcgctcgtgc
      421 tcggacggca cttccttgac gcgccgcagg tcgagcgggt ccgggtcacg atcgccgagc
      481 acgcctggcg cccgatcggc gcggcggtcg acgcgttcag ccgcgacggc tcggagacgc
      541 ggaccgcggc ggtcgtcgtc gggcgggacg gggcagcggt ccgggccggg atcgccgacc
      601 tgtcggtgat gaagacgacc cgctccgcgt tcgagggctt tccgcgcgac ggcttcacga
      661 ccctcaagga gacggccgac cggatcatgg cgacgaaggt gacggcaacg tgggcctacg
      721 agcccgcggc cgggctggat ttcgacgcct ccttcgccgg cgttcgatcc acctttctcg
      781 aggtcatggc cgatcaccac tcggtgtcgg tccagggctc catctggatc atcggcaagg
      841 cgatcctgga gcgtcatccc gaggtggccg aggtctcgat gtcgctgccg aacctccacc
      901 actggaccgt cgacctgagc ccgttcggga tcgcgaacga ccgcgcggtc tacatctcga
      961 cgacggagcc gcacgggctg atcgaggcaa cgatcaggcg cggcggatag gcgggcggcg
     1021 aactcgtccc cgttgccgga tgtccgtcgt gcgggcgtag tggcatgtat gggtcgagaa
     1081 tcgggtcgat ggtcgaatgc gtcacgcacg aagcgcggcg gcgccgcctt gtaacgcccg
     1141 tggtgcggac atccggtgag atccgtggcc ggcgcgggga gtaacgcccg tggtgcggac
     1201 atccggcaag aacctgcggt ggtgccgacg ggcgccgcac cgagagggca gcgcgcctgt
     1261 gacacgctag ctgtcacagg cgcaatcgag catgcgagcc atggaagccc gcatcgtcca
     1321 gctcccgacc aacgagatcc gggtcgacct ggaccggatc gtcatcgagc gtctcatcgt
     1381 tcgcgatccg gcgctggccg cgatcgtcgc cggacgggac gccgaggacc ggccggcgtt
     1441 cgtcgagcgc gccctgaaga tcggcctgac cgcgctgcag gacgcgaccg tcagcgtcga
     1501 tgtcgatctc gtccgcggcg agttcgacaa gctcctccgc tcctccgagc aggcgaacgc
     1561 caaggcggcc gaggcgctcg accaggtcct ccgctcgaac ttcgccgacg gcgacgggcg
     1621 gctgccgcgg acgctggaga agttcctggg cgatcgcggc gccctgcggt cgttcgtcgg
     1681 cgagctcttc gacgagacga agcgcgacag cgcgatcggc cggatgcgga cactgctcgg
     1741 gacctacttc gacggcgatg cgtcgaggct cgcgctcctc ctcgacccga cccgccagca
     1801 ctccccgatg caccagttcc ggaccgaggt gacggccggc ttcgacaagc tccacgagcg
     1861 cctcaccgcg atcgaggcgg cagcggccgc gcgcggcacg gagcgggccc gctcggcggc
     1921 caagggcgcc gacttcgagg acgtcctcga ggcgatgctc gccgacgtgg cgcgcggctc
     1981 gggcgatctc gtcgatcgga ccggttgcga gaccggggag ctgctgcgct cgaagaaggg
     2041 cgacttcgtc gtgacagtcg atccggcccg gaccggtggt gcggacgtgc gcgtcgtcgt
     2101 cgaggccaag gaccgcccga tgtcggtccg ctcgatccgc gaggagctgc gcgaggcgaa
     2161 gcagaatcgg gccgccgctg tcgggctggt cgtgttcagc ccggcccatg cgccgtcggg
     2221 gatcgcgccg ttcgacgtcc gcgccggcga cgtctactgc gtcatcgatc ccgccgaccc
     2281 ggatccagcg acgctcgaag cggccctccg cctggcccgg ctcctcgcga tcaccagcct
     2341 tcgcgacgtg gaggccgagg tcgacgtcga ggccgtgcga gcggccctcg ccgccgtccg
     2401 ggcggaactc gatgccctca aggggatcaa gtcgaccctc acctcgatct cgtcgagtgc
     2461 gtccgggctg cagatcacgc tggaccggct gcgcgatggc gtgatcgccc gggttgccga
     2521 cgccgaggcc gagatccggg ccgctcgcgc cgcagccaac tgaccctcgg gcggcaggtc
     2581 ggcatcgacg ctggtccgtt ggcgagttgc tccgttcggg gtggaggact agggtctgat
     2641 gtggttggga cgtgcggccc atccagcgat gggaccaccg ggtcctgtca tcgaccagcg
     2701 tcc
//
LOCUS       DATAIW010000316        10899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_187377, whole genome shotgun sequence.
ACCESSION   DATAIW010000316 DATAIW010000000
VERSION     DATAIW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187377"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VHM48_09740"
     CDS             complement(<1..1257)
                     /locus_tag="VHM48_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755735.1"
                     /translation="MGVGPATHEGPARPLSGRRVLLALAATVLALAGTFAPAHVPIAR
                     AASDGLELTTGATYTISPARHVVHVALAVTARNNKPNVTSGGVVTKYFYDGARVAIQP
                     EARNIRATSTGVRLTTSTKAADGYLVLEVRFRASLFYQQSTRIDITFDLPGGAPRSKS
                     DIRVGSAFATFVAWAFGDEGSVRVVVPKGFDAEATGSDATKSTSAGTTVFRSTGISDV
                     GSWYLVVNADRESALTNERVDLSGGEHIVIRAWPEDAAWQKRVSALLTTGLPELVEQT
                     GLNWPVTGDLSIFEVHTPLLEGYAGVFLQGEDKIEISEDLDDLTILHEASHAWFNGDL
                     FDGRWINEGFADTYAAKALDGIGSPSFTPGTVSPTDKAAVRLVDWVHPGRITDAETDA
                     REQYGYDASWTVIRSIVREVGDPGMQH"
     gene            complement(1263..3305)
                     /locus_tag="VHM48_09745"
     CDS             complement(1263..3305)
                     /locus_tag="VHM48_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755736.1"
                     /translation="MNRSPARRHGLVVGLALLAAVISIPAAALLPAGAPFAVPAAAAA
                     TGGLTMTADARYTVDPARRRVHVAVALAATNHRTDTKTRRYFFDRAFLAVQPGTTGYK
                     VTTPGAHPTVHVQRRASTYTLLRIDFGKQLGAGGTRRFKLTFDITDPGGAATRTTRIG
                     TTLVSFGAWGFGSAGNPGGSVTVVFPAGFSIDVDAPLLGKPATDTFGNVTYATGRLAS
                     PLTFFAYFVADRPGAYKETTLRVTVDGKAVPITLRAWPDDPTWAKRVGSLLTRGLPAL
                     AKDIGLPWTVEQPLIVSEAISRNATGFSGRYNPPAGEIEIAYYATSFVVLHEAAHAWF
                     DGGLLADRWASEGFASYYALRAAKAIGQKKVTGDVLTPALEKSRVPLNSWSAAGEDDA
                     TVEDAEYAAALKLATLIAARAGTDGLTTVWQAIRERRAAYQPTGPRASLETSDAAPDW
                     RGLLDLLEERTAKTYDDLWKAWVVRSADEALLIDRANVGQRYAAVARQADTWLLPRVV
                     RDALRAWQFDQANELLDGASTALDDRDVVLDATAAAGLTPPSTMQTDFEGPRGFAAAS
                     AEAEAELAAINAYRQTVATRPAEPDMITRIGLWNSDPSALVAAAVAAFSAGDLRASVE
                     SSAYARTIWTTAADIGRNRVLAIGASLAAVLVAGWLLLRWFRDRSSRRHLPIADKG"
     gene            complement(3302..3919)
                     /locus_tag="VHM48_09750"
     CDS             complement(3302..3919)
                     /locus_tag="VHM48_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX2755737.1"
                     /translation="MPSSEPGALVRPADVERWLDELGLVPLARADRDGVTSWDLRLDG
                     RRRFDLPITVILDPSLALICWVHFAPPIGDAFRKSFRKLLRWNDEFPFVKFSLAEDDR
                     PVLASELALRTADRDELGLAIARSLLIADQLLEESAGWLWIGGRIPDRSDRVSRGAEL
                     IARYADRLAELIGEPTSAVSDAAASDAAASDAAASAADVVPVTPS"
     gene            complement(3973..5382)
                     /locus_tag="VHM48_09755"
     CDS             complement(3973..5382)
                     /locus_tag="VHM48_09755"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF003670.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX2755738.1"
                     /translation="MKRTMAVILAGGEGERLSILSGVRAKPAVPFGGKYRIIDFTLSN
                     CVNSDITNVVVLTQYNPRSLNDHIGLGRPWDLDRTTGGVKLLQPYIARGRVAEWYRGT
                     ADAVLQNFNVIEHDAGDVILVLAGDHIYKMDYQPFIAAHKRKRADVTIAVRQVPLAEA
                     SRMGILALDDADRVVEWQEKPKHPKSDLASMGVYVFSKRALRTWLSEDRIDFGRDVIP
                     AMLDGGARVFGYHFEGYWQDVGTIQSYWEANLALLEDHAALDLYDRDWLIHTRSEERA
                     PAQVGPTAQVHRSLISHGCVINGTVVNSVLSPGVRVDVGAVVRDSIVMFDSVIRSGAV
                     IDRAILDKEVVVGSGAIVGDGPDFDTPNRQEPGRLNTGITVVGKRSIIPRGVRIGRNV
                     KIAGDVKAADFPSRNVKSGSSVESARPRTAAARIAAANRAAETRIAVPVDASTNGKRE
                     TVPSVRTARTVVTGGGQKG"
     gene            complement(5488..6900)
                     /locus_tag="VHM48_09760"
     CDS             complement(5488..6900)
                     /locus_tag="VHM48_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX2755739.1"
                     /translation="MRPSAHTDKNPSRSAESSRTGDQPRPAAATRQGRRRPRGLMRLP
                     LLVLIPLLVGLFGGPTSPAGADELSDARARQTALAQQLKDQKRQVAEINALQADLSSQ
                     IDSTRGQLSGINADLSSVKKSITSMGVKINIVRRNYLAQVATLQQLDIQLDRITVEES
                     VMTVNLRERKALLAERLRQAYDTDRTSMLETFLSAGSFTDVLSQVSYTIDVGEQDKQL
                     AEQIVHDQATLGAVHESVIETRQATDDLRVATAAQKVKLDAALKELKVAQKELKRLEA
                     ETARALAVQRDAYRKLAANKHNLAKAIAAAGHAKAVLAKRISNLVAAAYAHGNIPSQY
                     NGTLRWPMVGTVSQDFGCTGMTWEPPLGSCPHFHQGIDIVAPYGTAVRASGNGTVVYC
                     GWNYADGADPAWIVIIGHSQSLETWYAHMLPNCPAGAGTAVHAGQVIGHEGNTGHSTG
                     AHLHWAVRFNNDFVNPRLFL"
     gene            complement(7222..7866)
                     /locus_tag="VHM48_09765"
     CDS             complement(7222..7866)
                     /locus_tag="VHM48_09765"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX2755740.1"
                     /translation="MNFLAVVLALGGAAFGVAADRVAVRWPAHDDEHPAGRSVGWRTI
                     ASGAIGAFAFWLLGERFADADPIVQAVFGLWFAALVLGFAVDLDQRLLPDELTLPIIP
                     LALFLDVIGRNPLVGTELLPAIAIAVAVPLVIYAASIPFGAGAFGLGDVKLLVGVGLM
                     SGFLRTFTGLLSGLFAAGLVLAVLLTTRRIGRRTFVPFGPFLIFGALWGIFTRV"
     gene            complement(7883..9397)
                     /locus_tag="VHM48_09770"
     CDS             complement(7883..9397)
                     /locus_tag="VHM48_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2755741.1"
                     /translation="MHLRVVTDQPWDVKADVLAIPIVGEPAFEGPLGELNKRTGGELA
                     ALAAFGELRGKRFTSSVAAPGELAAGRVLTVSAGEPTKLDRETVLHVGAAAEHRLGGR
                     KARSLAIWLTPLADVLDGGVEAVAELVARGVVEGSFEPRTLYRDHDDVATAPPILDEL
                     ILIAPGADAGAAHKAAERGVIIGQGANHAKSLSNRAANDVSPEVLAQEARDVAAKHGL
                     WIDVIEPDRARELGMGMFLAVGQGSDNPPRMIVMRSGGEGEKDALDRHLAIVGKGVCF
                     DSGGISIKPSDRMEEMKMDKIGACTVIAAIATVARLAPGTPLLAVAPAVENMPGPHST
                     RPGDVVKALNGKWVDITNTDAEGRLILGDAMTYAERLGATHIVDVATLTGAVSRAFGH
                     LITGAFGTPQGWYDDVMGAAAKAAERYWQIPLLDDYVTDMESWYGDLQNSGSAEGSLI
                     KSGLFLREFRTVPWAHLDIGGTGYFRKTTPYAPRGATGVTHATLVELALAGAKT"
     gene            complement(9473..10474)
                     /locus_tag="VHM48_09775"
     CDS             complement(9473..10474)
                     /locus_tag="VHM48_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755742.1"
                     /translation="MTRFLQRAALAVVWLLIVAVVSVGGAGLVAAMANQPATPARAEL
                     TADGDAAAVAALDGIQVELSDLTKDVERLGELGRGALTALVSSDFATLDSAVADGQGL
                     ARAIEARSGQILGEVRLLSGTGPNEALVWSPETSQRRDLALAAVNATGGLELAWTRFA
                     SGSTVANRLTILLTNHDTTAGKAAATGRDRKYAAALKTLATAQAMLDEVKTLRDALAN
                     TVDVNTLTQWIDRNAEYDAALARLYKATIAAKGKITNELKSAFAAERKAHDFLPSNTT
                     GLVIILAEIGRGGLNQAVIGIEETRAKLQAAVDRLSAPVTVEPDASDDGIEPSPSGG"
     gene            10498..>10899
                     /locus_tag="VHM48_09780"
     CDS             10498..>10899
                     /locus_tag="VHM48_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755743.1"
                     /translation="MDDLPSAPARNPAPPVRIAEYDGGSAAGPTGAADAAEQPDRRAH
                     LHAMPKAELHLHLDGSLRIETALDLARTRGVDAPRDGAGIRAVLVAPMPCRNQAELLR
                     AFDLPIALMQDADALERITAELVETKAAENVR"
BASE COUNT         1556 a         3727 c         3974 g         1642 t
ORIGIN      
        1 gtgctgcatc ccggggtcgc cgacctcccg gacgatggat cggatgaccg tccacgatgc
       61 gtcgtagccg tattgctcgc gggcgtccgt ctccgcgtcg gtgatccggc ccgggtgcac
      121 ccagtcgacg agccggaccg cggccttgtc tgtcgggctg acggtgccgg gagtgaagct
      181 cgggctgccg atcccgtcga gcgccttcgc ggcatacgtg tcggcgaagc cctcgttgat
      241 ccagcgaccg tcgaacaggt cgccgttgaa ccaggcgtgc gaggcctcgt ggaggatcgt
      301 gaggtcgtcg aggtcctcgc tgatctcgat cttgtcctcg ccctggagga acacgccggc
      361 gtagccctcg agcagcgggg tgtggacctc gaagatcgac aggtcgccgg tgaccggcca
      421 gttcagcccg gtctgctcga cgagctcggg cagtccggtg gtgagcaacg cgctgacgcg
      481 cttctgccag gcagcgtctt ctggccacgc ccggatgacg atgtgctcgc caccgctcag
      541 gtcgacccgt tcgttggtga gggcgctctc gcggtccgcg ttgacgacga ggtaccagct
      601 cccgacatcg ctgatcccgg tcgaccggaa gaccgtcgtt ccggcgctcg tcgacttcgt
      661 cgcatcggag ccggtggcct cggcgtcgaa gcccttgggg acgacgaccc ggacgctgcc
      721 ctcgtcgccg aaggcccacg cgacgaacgt ggcgaacgcg gacccgaccc ggatatcgct
      781 cttcgatcgc ggcgccccgc ccggaaggtc gaaggtgata tcgatccggg tcgactgttg
      841 gtagaagagc gacgcccgga atcggacctc gaggacgaga taaccgtcag ccgccttcgt
      901 cgacgtcgtc agccggacgc cggtcgacgt cgcccggatg ttccgcgcct cgggctggat
      961 cgcgacccgg gcgccgtcgt agaagtactt cgtgacgacc ccgccggagg tcacgttcgg
     1021 cttgttgttg cgggcggtga cggccagcgc cacgtggacg acatggcggg ccggcgagat
     1081 cgtgtaggtc gcgccggtgg tgagctcgag gccgtcgctg gcggcccgcg cgatcggcac
     1141 gtgggccggg gcgaacgtgc cggcgagggc gaggacggtc gcggcgaggg cgaggaggac
     1201 ccgccgcccc gacaacggcc gtgcggggcc ctcatgcgtc gccggtccga cccccatgac
     1261 gatcagccct tgtcggcgat gggcaagtgg cggcggctgc tccggtcccg gaaccagcgc
     1321 agcaggagcc agccggcgac gagcaccgca gccagcgatg cgccgatggc gaggacgcgg
     1381 ttgcgaccga tgtcggcagc ggtcgtccag atcgtccggg catacgccga cgactcgacg
     1441 ctcgcgcgaa ggtctccggc cgagaacgcc gcgaccgcgg ccgctaccag cgcggacggg
     1501 tcgctgttcc agagcccgat gcgcgtgatc atgtccggct cggcgggtcg ggtggcgacg
     1561 gtctggcgat acgcgttgat cgcggccagc tccgcctcgg cctcggcgga cgcggccgca
     1621 aagccgcgcg gcccctcgaa gtcggtctgc atcgtggacg gcggcgtgag cccggcggcg
     1681 gcggtcgcat cgagcacgac gtcgcggtca tcgagcgcgg tgctcgcccc gtcgaggagc
     1741 tcgttcgcct gatcgaactg ccaggcgcgc agggcgtcgc gcacgacccg cggcaggagc
     1801 cacgtgtcgg cctgccgggc gacggccgcg tagcgctggc cgacgttggc gcggtcgatg
     1861 agcagcgcct cgtcggccga ccgaacgacc cacgccttcc acaggtcgtc gtaggtcttg
     1921 gcggtccgct cctcgagcag gtcgagcagc ccgcgccagt cgggggctgc atcgctcgtt
     1981 tcgaggctcg cccgcgggcc ggtcggctga taggccgcgc gtcgctcgcg gatcgcctgc
     2041 cagaccgtcg tcagcccgtc cgttccggcc cgtgcggcga tcagggtcgc gagcttgagc
     2101 gccgcggcgt actcggcatc ctcgaccgtg gcgtcgtcct cgcccgcggc ggaccacgag
     2161 ttcagcggca cacgcgactt ctccagagcc ggagtcagga cgtcgccagt caccttcttc
     2221 tggccgatcg ccttcgcggc acgcagggcg tagtacgacg cgaacccctc gctcgcccaa
     2281 cgatcggcca ggagcccgcc gtcgaaccag gcatgggccg cctcgtggag gaccacgaac
     2341 gacgtcgcgt agtaggcgat ctcgatctcg ccggccggcg ggttgtagcg tccggagaag
     2401 ccggtcgcat tgcggctgat ggcctccgac acgatcagcg gctgctcgac cgtccagggc
     2461 aggccgatgt ccttcgccag ggccggcagg ccgcgggtga gcaggctgcc gacccgcttg
     2521 gcccaggtcg ggtcgtccgg ccaggcgcgg agcgtgatcg ggacggcctt gccgtcgacg
     2581 gtgacccgga gcgtcgtctc cttgtaggcg cccggccggt ccgcgacgaa ataggcgaag
     2641 aaggtcagcg ggctcgcgag gcggcccgtg gcataggtca cgttgccgaa ggtgtcggtc
     2701 gcgggcttgc cgagcaacgg ggcgtcgacg tcgatcgaga acccggccgg gaacacgacc
     2761 gtgacgcttc cgcccgggtt gcccgcgctg ccgaagcccc aggccccgaa gctcacgagc
     2821 gtcgtcccga tgcgggtcgt ccgggtcgcc gcgccgccgg gatcggtgat gtcgaaggtc
     2881 agcttgaagc ggcgcgtccc gccggcaccg agctgcttgc cgaagtcgat ccggagaagc
     2941 gtgtacgtgc tcgcccggcg ctggacgtgg accgtcggat gggcgcccgg ggtcgtgacc
     3001 ttgtagccgg tcgtgcccgg ctggacggcg aggaacgccc ggtcgaagaa gtaccggcga
     3061 gtcttcgtgt cggtccgatg gttcgtcgcc gcgagggcga ccgcgacgtg gacgcggcgt
     3121 ctggccgggt cgaccgtgta gcgcgcatcg gcggtcatcg tcaggccgcc ggtcgcggcc
     3181 gcggcagccg ggacggcgaa cggcgcgccg gccgggagga gggcagccgc cgggatcgag
     3241 atgacggcgg cgagaagcgc gagcccgacg acgaggccgt ggcgccgggc tggtgagcgg
     3301 ttcacgaggg ggtcaccgga acgacgtcgg cggcggatgc cgcggcgtcg gaggctgcgg
     3361 cgtcggaggc cgcggcgtcg gagaccgctg acgtcggctc cccgatcagc tcggcaagtc
     3421 gatccgcgta acgcgcgatc agctcggcgc ccctgctgac cctgtccgac ctgtccggga
     3481 tccggccgcc gatccacagc caacccgccg actcctcgag cagctggtcg gcgatcagga
     3541 gggagcgtgc gattgcgagc ccgagctcat cgcggtcggc ggtccgcagc gcgagctcgc
     3601 tcgccagcac aggccgatcg tcctccgcca gggagaactt gacgaacggg aattcgtcgt
     3661 tccagcgaag cagcttccgg aaggacttgc ggaatgcatc gccgatcggc ggggcgaagt
     3721 ggacccaaca gatcagcgcc agcgacggat cgaggatcac cgtgatcggc aggtcgaagc
     3781 gccgccggcc atcgagccgg aggtcccacg acgttacgcc gtcgcgatcg gcccgcgcca
     3841 ggggcacgag gcccagctcg tcgagccagc gctcgacatc cgccgggcgc acgagggcac
     3901 ccggctctga agaaggcatg gcgggcagtc taccgtcagt cctcgatccc ggctgccggg
     3961 acgagtccgc gctcagccct tctggccgcc cccggtgacg accgtccgcg ctgtccgcac
     4021 ggatggcacg gtctcgcgct tgccgttcgt cgaggcgtcg acggggaccg cgatccgcgt
     4081 ttcggccgca cggttggcgg cggcgatccg ggcggccgcg gttcgcggcc tggccgattc
     4141 gaccgacgat ccgctcttga cgttccggct cgggaaatcg gccgccttga cgtcgcccgc
     4201 gatcttgaca ttgcgcccga tccggacgcc gcgcgggatg atcgagcgct tgccgacgac
     4261 cgtgatgccg gtgttcagcc gtcccggctc ctgcctgttc ggggtgtcga agtccggccc
     4321 gtcgccgacg atcgcgcccg acccgacgac gacctccttg tcgaggatcg ctcgatcgat
     4381 gaccgccccc gagcggatga ccgagtcgaa catcacgatc gaatcacgga cgaccgcgcc
     4441 gacgtcgacc cggacgcccg gcgagaggac ggagttgacg acggtcccgt tgatcacgca
     4501 gccgtggctg atgaggctgc ggtggacctg cgccgtcggg ccgacctggg ccggggcgcg
     4561 ttcctcggac cgggtgtgga tcagccagtc gcggtcgtaa aggtcgagtg cggcgtgatc
     4621 ctcgagcagg gcgaggttgg cttcccagta ggactggatc gtgccgacgt cctgccagta
     4681 gccctcgaag tgatagccga agacgcgggc gccgccgtcc agcatcgccg ggatgacgtc
     4741 cctgccgaag tcgatccggt cctcgctgag ccatgttcga agggcccgct tggagaacac
     4801 gtagacgccc atcgaggcga ggtcgctttt ggggtgcttc ggcttctcct gccattcgac
     4861 gacccggtcg gcgtcgtcga gggcgaggat gcccatccgc gacgcctcgg ccaggggcac
     4921 ctggcggaca gcgatcgtga cgtcggcgcg cttccgcttg tgggccgcga tgaacggctg
     4981 gtagtccatc ttgtagatgt ggtcgccggc gaggacgagg atcacgtcgc ccgcgtcgtg
     5041 ctcgatcacg ttgaagttct ggaggaccgc gtcggccgtc ccgcggtacc actccgccac
     5101 ccggccgcgg gcgatgtacg gctggaggag cttcactcca ccggtcgtcc ggtcgaggtc
     5161 ccacggccgg ccgaggccga tgtggtcgtt caacgagcgg gggttgtact gggtcaggac
     5221 gaccacgttg gtgatgtccg agttgacgca gttcgacagg gtgaagtcga tgatccggta
     5281 cttgccgccg aacgggaccg cgggcttggc ccggacgccc gagaggatgg acaggcgctc
     5341 gccttcaccg ccggcgagga tgacggccat cgtccgcttc atatgcggat gctaacaccg
     5401 tggcgagtcg ttccggtcat cgggcgatga cggttcgatt gcgatcccgt gatcgtcgcc
     5461 ccaacgtgcg cgcgccggcg ccgcgcccta gaggaacagg cgcgggttga cgaagtcgtt
     5521 gttgaagcgg acggcccagt ggagatgggc gccggtcgag tggcccgtgt tgccctcgtg
     5581 gccgatcacc tggcccgcat ggaccgcggt cccggcaccg gcggggcagt tcggcagcat
     5641 gtgggcatac cacgtctcga gcgactggct gtggccgatg atcacgatcc aggccgggtc
     5701 cgcgccgtcg gcgtagttcc agccgcagta gacgacggtg ccgttgccgg acgcccggac
     5761 cgcggtcccg tacggggcga cgatgtcgat gccctggtgg aaatgggggc aggagccgag
     5821 cggcggctcc caggtcatgc cggtgcagcc gaagtcctgg ctgacggtac cgaccatggg
     5881 ccagcgcaac gtcccgttgt attgcgaggg aatgttgccg tgggcgtagg cggccgcgac
     5941 gaggttgctg atccgcttgg ccagcaccgc cttggcgtgg ccggcggccg cgatcgcctt
     6001 ggcgaggttg tgcttgttgg cggcgagctt gcggtacgcg tctctctgga ccgcgagcgc
     6061 tcgtgccgtc tcggcctcga ggcgcttcag ctccttctgg gcaaccttca gctccttgag
     6121 cgcggcgtcg agcttgacct tctgggcggc ggtcgcgacc cgcaggtcgt cggtcgcctg
     6181 gcgcgtctcg atcacggatt cgtggaccgc gccgagggtc gcctggtcgt ggacgatctg
     6241 ctcggccagc tgcttgtcct gctcgccgac gtcgatcgtg tagctgacct gggacaggac
     6301 gtcggtgaag gacccggccg acaggaacgt ctcgagcatc gaggtccgat cggtgtcgta
     6361 ggcctggcgc aggcgctccg ccaggagggc cttgcgctcg cgcaggttca cggtcatgac
     6421 cgattcctcg accgtgatcc ggtccagctg gatgtcgagc tgctggagcg tcgccacctg
     6481 ggcgaggtag ttgcgccgga cgatgttgat cttgacgccc atcgacgtga tcgacttctt
     6541 gaccgacgac aggtcggcgt tgatgccgct cagctggccg cgcgtcgagt cgatctggga
     6601 gctgaggtcc gcctggaggg cgttgatctc ggcgacctgt ctcttctggt ccttcagctg
     6661 ctgggcaagc gcggtctggc gcgcacgggc gtcactgagc tcgtccgcgc cggccggcga
     6721 ggtcggtcca ccgaagagcc cgacgaggag cggaatgagg acaagcaacg gcagacgcat
     6781 caggccgcgc ggccggcgtc gaccctggcg ggtcgcggct gcgggtcgcg gttgatcgcc
     6841 tgtccggctg gactcggcgc tgcgtgacgg gttcttgtcc gtgtgcgcgc tggggcgcat
     6901 cacaggagcg atgtcatcgt gcgagcaaac tccgttgttc gcgtctcgct gcgcaggact
     6961 gcgcagttgc gcggttggcg gctacgcccg ccaattcgat cgccccgacg ggcaccctca
     7021 ggacgacgac cggctttccg gcatcatacc ccgggcgcca actgtagcca agctgtaacc
     7081 cctgggccac cctgactttg gtcccatcac tgcctcggac cccaccggag tgcaccggac
     7141 ggcactgcac aattcgggtc gacttcacgg tcgatgagac cgtccatggc gtccggcgct
     7201 gggcgtcggc gcgcgaccac ctcagacccg ggtgaagatc ccccacagcg cgccgaagat
     7261 caggaacggc ccgaacggca cgaacgtccg ccgcccgatc cgccgtgtcg tgagcagcac
     7321 tgcgaggacc aaccccgcgg cgaacagacc ggacagcaga ccggtgaatg tccgcaggaa
     7381 cccgctcatc aggccaaccc cgaccagcag cttcacgtcg cccagcccga acgccccggc
     7441 accgaacgga atcgaggccg cgtagatcac gagcggcacg gcgaccgcga tcgcgatcgc
     7501 cggcagcagc tcggtgccga cgagcgggtt ccggccgatg acgtcgagga agagcgccag
     7561 cgggatgatc ggcagggtca gctcgtcggg caggagccgt tgatcgagat cgacggcaaa
     7621 gccgaggacc agcgccgcga accacagccc gaacacggcc tggacgatcg ggtccgcgtc
     7681 agcgaaccgt tcgccgagca gccagaaggc gaacgccccg atcgcgccac tggcgatggt
     7741 ccgccagccg accgatcggc cggccgggtg ctcgtcgtca tgcgccggcc agcggacagc
     7801 gacgcggtcg gccgccacgc cgaacgccgc cccgccgagg gcgaggacga cggccaggaa
     7861 gttcaccggt ccgagcctgg gctcaggtct tcgccccggc cagcgcgagc tcgacgagcg
     7921 tggcgtgggt cacgccggtc gcgccccgtg gcgcgtacgg tgtcgtcttc cggaaatagc
     7981 ccgtcccgcc gatgtcgaga tgggcccacg gcaccgtccg gaactcgcgc aggaagaggc
     8041 cgctcttgat cagcgagccc tccgcagaac cggagttctg gaggtcgccg taccagctct
     8101 ccatgtccgt gacgtaatcg tcgaggaggg ggatctgcca gtaccgctcg gcggccttcg
     8161 cggcggcgcc catcacgtcg tcgtaccagc cctggggcgt cccgaaggcc ccggtgatca
     8221 ggtggccgaa ggcgcgcgag accgcgcccg tcagcgtcgc gacatcgacg atgtgggtgg
     8281 ctccgaggcg ctccgcgtag gtcatcgcgt caccgaggat cagacggccc tcggcgtcgg
     8341 tgttggtgat gtcgacccat ttgccgttga gcgccttgac gacatcgccg ggtcgggtgg
     8401 agtgcggccc gggcatgttc tcgaccgccg gggcgaccgc caggagcggc gtacccgggg
     8461 ccaggcgggc caccgtggcg atggccgcga tcacggtgca ggcaccgatc ttgtccatct
     8521 tcatctcttc catccggtcg gacggcttga tgctgatgcc gcccgagtcg aagcagacgc
     8581 ccttgccgac gatcgcgaga tggcggtcga gggcgtcctt ctccccctca ccgccggaac
     8641 gcatgacgat catccggggc gggttgtcgc tgccctggcc gaccgcgagg aacatcccca
     8701 tgccgagctc gcgggcacgg tccggctcga tcacgtcgat ccacaggccg tgtttcgcgg
     8761 cgacgtcgcg ggcctcctgg gcgaggacct cggggctgac gtcgttggcc gcgcggttcg
     8821 agagcgactt ggcgtggttg gcgccctgcc cgatgatcac gccacgctcg gcggccttgt
     8881 gcgccgcgcc ggcatcggca ccgggagcga tgaggatcag ctcgtcgagg atcggcgggg
     8941 cggtcgcgac gtcgtcgtgg tcgcggtaga gggtcctggg ctcgaagctg ccctcgacga
     9001 ccccgcgggc gacgagctcc gcgacggcct cgacgccgcc gtcgaggaca tcggcgaggg
     9061 gcgtcaacca gatcgcgagg ctgcgggcct tgcggccacc gaggcgatgc tcggccgcgg
     9121 ctccgacgtg gaggaccgtc tcgcgatcga gcttggttgg ctcgccggcg ctgaccgtca
     9181 ggacgcggcc ggccgcgagc tcacccgggg cggcgaccga cgaggtgaac cgcttgccgc
     9241 ggagctcgcc gaaggccgcc agggccgcca gctcgccgcc ggttcgcttg ttgagctcgc
     9301 cgaggggtcc ctcgaacgcc ggctcgccga cgatcgggat ggcgaggacg tcggccttga
     9361 cgtcccatgg ctggtcggtg acgacgcgca gatgcacgga agggcactcc tggcagagag
     9421 gtcgcgccca ggttggcggg cgcggacgga gacagtcccg gcagtgtacc cgtcagccgc
     9481 cggacggcga gggctcgatg ccgtcgtcgg atgcgtccgg ttcgaccgtg acgggcgcgc
     9541 tcaggcgatc gaccgccgcc tgcagcttgg cccgcgtctc ctcgatgccg atcacggcct
     9601 ggttgagccc gccccgaccg atctcggcga ggatgatcac gagcccggtc gtgttcgagg
     9661 gcaggaagtc gtgggccttg cgctcggccg cgaaggcgct cttgagctcg ttggtgatct
     9721 tgcccttggc cgcgatcgtc gccttgtaga ggcgggcgag ggccgcgtcg tactcggcgt
     9781 tgcgatcgat ccactgggtc agcgtgttga cgtcgacggt gttggcgagg gcatcgcgga
     9841 gcgtcttcac ctcgtcgagc atcgcctggg ccgtggccag ggtcttcagc gcggccgcgt
     9901 acttgcggtc gcgcccggtc gcggccgcct tgcccgcggt ggtgtcgtgg ttcgtcaaca
     9961 ggatcgtcag ccgattggcg acggtcgagc ccgaggcgaa acgcgtccag gcgagctcga
    10021 gcccaccggt cgcgttgacc gcggcgagcg ccaggtcgcg gcgctggctg gtctcgggcg
    10081 accacacgag cgcctcgttc gggcccgttc ccgacaggag ccggacctcc ccgaggatct
    10141 ggccggaccg cgcctcgatc gcccgggcga gcccctgacc gtcggcgacg gcgctgtcga
    10201 gggtcgcgaa gtcggacgag acgagggcgg tgagcgcgcc gcgcccgagc tcgccgagcc
    10261 gctcgacatc cttcgtgaga tcggacagct cgacctggat cccgtcgagg gccgcgacag
    10321 ccgcggcgtc accgtcggcc gtgagctcgg cccgggccgg ggtcgccggc tggttggcca
    10381 tcgccgcgac gaggccggca ccgccgaccg agaccactgc cacgatcagc agccacacga
    10441 cggcgagcgc ggcgcgctgg aggaaccggg tcatcgccgg catcgtagca tcgggccatg
    10501 gacgacctgc cgagcgcgcc tgcgcgcaac cccgcgccgc ccgtgcggat agccgaatac
    10561 gacggggggt cggccgcggg cccaaccggc gcggctgacg ccgccgagca gcccgatcgc
    10621 cgcgcccacc tccatgcgat gcccaaggcc gagctccacc tccatctcga cggctcgctc
    10681 cggatcgaga ccgccctcga cctggcccgg acgcggggcg tcgacgcgcc gcgcgatggc
    10741 gccgggattc gggccgtcct cgtcgccccg atgccgtgcc gcaaccaggc ggagctcctc
    10801 cgcgccttcg atctcccgat cgccctgatg caggacgccg acgcgctcga gcggatcacc
    10861 gcggagctcg tcgagacgaa ggcggccgag aacgtccgc
//
LOCUS       DATAIW010000317         4850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_169067, whole genome shotgun sequence.
ACCESSION   DATAIW010000317 DATAIW010000000
VERSION     DATAIW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169067"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..882
                     /locus_tag="VHM48_09785"
     CDS             100..882
                     /locus_tag="VHM48_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755744.1"
                     /translation="MPHVSDVRPTSHDRHDPILVAALAADDLTGIDRDQAIAQTRSCA
                     ECATLRDDLVALARATASAPPPFATPPRDFRLTPADAARLRPTGWRRFVAALSMPRSA
                     ATRPLGVGLATLGLVGLLVGNVQLGSSAGVPQGASTTAGSLAAPLPNADMVQVPSAGA
                     AAGQVPAAAASAAPATGSSSEIYGGAVASSLPDMSRGAPITAADSSGKAAATEMLPGD
                     AQAGRDAVVGEPFRPLNVLFGAAVVVGIGLLVGSRLRGRRPV"
     gene            941..3895
                     /gene="polA"
                     /locus_tag="VHM48_09790"
     CDS             941..3895
                     /gene="polA"
                     /locus_tag="VHM48_09790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2755745.1"
                     /translation="MPTERLMLLDGNGLIYRGYFALLETPLTTSKGELVNAVFGFTNI
                     VLRALADVKPDYVAIAFDLGAPTFRHERYLEYKATRTRMPDDMRDQIPKVREVVEALN
                     IPKYEREGFEADDVIATLVGQAEAADLYTTILTGDLDMLQLVTDRTTLMVSLRGGIAN
                     TVAYDRAKIDDRWGLRPDQMLDYKSLKGDPTDNIPGVPGIGEKTASKIIKTFGTLDAM
                     YERLDELEPAKFREPLAAAKETVLESRELMRLVTAPDIQLDLPGARVGSYDRERVVRL
                     FREYEFRTLIERLPALRGEDPIAAAQALREVVARGTVGAARTPGSQPRPGDWGPGRAT
                     ARPARPEGSAELQLSLDFGAVAAADTNGNGAASQPRATPATPIDDLPQALAAAIDDPS
                     RIELHDVDGVAALVPWIAAQPAIGVGYLLDDPRPRMGSALALALVGMDGRVVAVEGVD
                     AVAALRRALEASGTPIVGHEVKPLLVARIADDQAAPGTPVAFDTQIAAYILNASLRSQ
                     TIADVAAEQLDLILPPAKDLSHAARAGLEALSAMAVREPLERRLADEALARLFVEIEL
                     PLIPVLARMEAVGVVIDREALAGLATEFGEVISRLEQEIYIDVGHEFNLGSPKQLEQI
                     LFFELNLPKGRRTKTGYSTDASVLEDLRPAHPMIDKLLEWRVYTKLRSTYVEALPSLI
                     AADGRLHTTFHQAVAATGRLSSSDPNLQNIPIRTPLGRRIRRAFVAGGPDVTLVAADY
                     SQVELRILAHVSGDEHLRDAFARNADIHRETAARVLHKAPEDVTHDERSMAKMVNFGL
                     AYGMSDFGLASRANIPRAEAQEFINSYFASYSGISYYMMAIKEQAKTQGFVTTLLGRK
                     RSIPELQARNPTLRSAGERMAINMPIQGTAADIQKIAMIRLDERLRAGAFRSRLLLSV
                     HDELLLEVPRDELDRLVPLVRETMESALALDVPLTVDVKVGDDWESMTPLTRADAIAA
                     EAAEAPAEPIAG"
     gene            3907..>4850
                     /gene="mutM"
                     /locus_tag="VHM48_09795"
     CDS             3907..>4850
                     /gene="mutM"
                     /locus_tag="VHM48_09795"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2755746.1"
                     /translation="MPELPEVETVVRDLRPLITGATIVDATTLWARTLRGMSAEAFAD
                     GVAGRRIESVGRRAKQIVIDLSGGAFLTIHLKMTGQLFVVPQGGPEDPYVRLVLFLED
                     GREIRFRDIRKFGKVGLYGADSSVAEADPFARIGPEPLADAFTVAVLRRRIRERKGRL
                     KPLLLDQSFLAGIGNIYADEALWAARLHPLRTARTLRPADERRLWLEIRRILAEAVIR
                     RGSSIDDYTAPDGDGEMQDHLAVYQQTGEPCLRCGRPIRRIVVGARATHFCSWCQRLP
                     ATDRSGARAILRTASTPERPGRRWSELPGGEGSVGLARA"
BASE COUNT          692 a         1836 c         1650 g          672 t
ORIGIN      
        1 gaccggctcg ccgaccggat ggagctgttc cgtggctgat cgacgaagtt cctgcccggt
       61 gatcgggaac cggacccgcg tggaggtcgt ctgaaccgca tgccacacgt ttccgacgtt
      121 cgcccgacgt cgcacgatcg ccacgacccg atcctcgttg ccgcgctcgc cgccgacgac
      181 ctcaccggca tcgaccgcga ccaggccatc gcccagaccc gctcctgcgc cgaatgcgcg
      241 accctccgcg acgacctcgt tgcccttgcc cgggcgaccg cgtccgcgcc gccccccttc
      301 gcgacccctc cgcgcgactt ccgattgacg ccggccgatg cggcgcggct ccggccgacc
      361 ggctggcggc gattcgtggc cgcgctctcg atgccgcgtt ccgcggccac acgcccgctc
      421 ggcgtcgggt tggccacgct cgggctggtc ggcctcctcg tcggcaacgt ccagctcggc
      481 agcagtgccg gcgtcccgca gggggcttcg actacagctg gttccctcgc agctccgctg
      541 ccgaacgccg acatggttca ggtcccgtct gctggcgccg cagcaggcca ggtgcccgcc
      601 gccgctgcgt cggccgcgcc tgccacggga tcaagctcgg agatctacgg aggggcagtc
      661 gcctcgtccc tcccggacat gagccggggc gccccgatca ccgcggccga ctcgagcggc
      721 aaggccgccg cgaccgagat gctccccggc gatgcgcagg cgggccgcga tgcggtcgtc
      781 ggcgagccgt tccgtccgct caacgtcctg ttcggggcgg ccgtcgtcgt cgggatcgga
      841 ctcctcgtcg ggtcgcggct gcgagggcgc cggcccgtct gaggcggtcg ttcgatggct
      901 ccggcgtggc cgcgcgcggg gccgcgggta cactccgcag gtgcccaccg aacgcctcat
      961 gctgctggac ggcaacgggc tgatctaccg cggctacttc gccctcctcg agaccccgct
     1021 cacgacgtcc aagggcgagc tcgtcaacgc ggtgttcggg ttcacgaaca tcgtgttgcg
     1081 ggcactcgcc gacgtgaaac ccgactacgt cgcgatcgcc ttcgacctcg gcgcgccgac
     1141 cttccggcat gagcgctacc tcgagtacaa ggccacccgg acccggatgc cggacgacat
     1201 gcgcgaccag atcccgaagg tccgcgaggt cgtcgaggcc ctgaacatcc cgaagtacga
     1261 gcgcgagggc ttcgaggcgg acgacgtcat cgccacgctc gtcggccagg ccgaggcggc
     1321 cgacctgtac acgaccatcc tgaccggcga cctcgacatg ctccagctcg tcaccgatcg
     1381 gacgacgctg atggtctcgc tccgcggcgg catcgccaac acggtcgcct acgaccgcgc
     1441 gaagatcgac gatcgctggg gcctccgacc ggaccagatg ctcgactaca agtcgctcaa
     1501 gggcgacccg accgacaaca tccccggcgt ccccgggatc ggcgagaaga ccgcctcgaa
     1561 gatcatcaag accttcggca cgctcgacgc gatgtacgag cggctcgacg agctcgagcc
     1621 ggccaagttc cgcgagcccc tggcggccgc gaaggagacc gtcctcgaga gccgcgagct
     1681 gatgcgcctc gtcaccgccc ccgacatcca gctcgatctg cccggcgccc gggtcggctc
     1741 ctacgaccgg gagcgggtcg tccgcctctt ccgcgagtac gagttccgga cgctgatcga
     1801 gcggctgccg gccctccgcg gcgaggaccc gatcgcggcc gcccaggcgc tccgcgaggt
     1861 cgttgccaga gggaccgtgg gcgcagcgcg gacgcccgga tcgcagccac ggcccggcga
     1921 ctggggccct gggcgcgcga cggcccgacc ggcccgcccg gagggcagcg cggagctcca
     1981 gctgtcgctc gatttcgggg ccgtcgctgc cgcggacacg aacggcaacg gcgcagcgtc
     2041 gcagccgcgg gcaacgccgg cgaccccgat cgacgacctg ccgcaggccc ttgccgccgc
     2101 gatcgacgac ccgtcccgga tcgagctcca cgatgtcgac ggggtcgccg cgctcgtccc
     2161 gtggatcgcg gcccagccgg cgatcggggt cggctacctg ctcgacgacc cgcggccgcg
     2221 gatgggctcg gcgctggctc tcgccctggt cgggatggac ggacgggtcg tcgcggtcga
     2281 gggcgtcgat gccgtcgccg ccctgcgccg cgccctcgag gcgagcggga ccccgatcgt
     2341 cggccacgag gtgaagccgc tgctcgtcgc ccggatcgcc gacgaccagg ccgcaccggg
     2401 gacgccggtc gcgttcgaca cccagatcgc cgcctacatc ctcaacgcgt cgctccgcag
     2461 ccagacgatc gccgacgtcg ccgccgagca gctggacctc atcctcccgc cggccaagga
     2521 cctcagccac gcggcgcggg ccggtctcga ggcgctctcg gcgatggccg tccgcgagcc
     2581 gctcgagcgc cgcctggccg acgaggccct cgcccggctc ttcgtcgaga tcgagctgcc
     2641 gctgatcccg gtcctcgcca ggatggaggc cgtcggggtc gtcatcgatc gcgaggcgct
     2701 ggccggtctg gcgacggagt tcggcgaggt gatctcccgg ctcgagcagg agatctacat
     2761 cgacgtcggc cacgagttca acctcggcag ccccaagcag ctcgagcaga tcctgttctt
     2821 cgagctgaat ctgccgaagg gtcgccggac caagaccggc tactccaccg acgcgtcggt
     2881 gctcgaagac ctccggcccg cccacccgat gatcgacaag ctcctcgagt ggcgggtcta
     2941 caccaagctt cgctcgacct atgtcgaggc gctgccgtcg ttgatcgccg ccgacggccg
     3001 gctccacacg acgttccacc aggccgtcgc cgcgaccggc cgcctgtcct cgtcggaccc
     3061 gaatctccag aacatcccga tccggacccc gctcgggcgg cggatccggc gggcgttcgt
     3121 ggccggcggg ccggacgtga cgctggtcgc cgccgactac tcccaggtcg agctgcggat
     3181 cctcgcccac gtctcgggcg acgagcatct ccgcgacgcc ttcgcccgga atgccgacat
     3241 ccaccgtgag acggccgcgc gggtcctcca caaggcgccc gaggacgtca cccacgacga
     3301 gcggtcgatg gccaagatgg tcaacttcgg tctggcctac gggatgagcg acttcgggct
     3361 cgcgtcgcgg gcaaacatcc cgcgcgccga ggcccaggag ttcatcaact cctacttcgc
     3421 gtcgtactcc gggatcagct actacatgat ggcgatcaag gagcaggcga agacccaggg
     3481 cttcgtcacg acgctgctcg gccggaagcg gtcgatcccg gagctccagg cccgcaatcc
     3541 gaccctgcgc tcggcgggcg aacggatggc gatcaacatg ccgatccagg ggacggcagc
     3601 cgacatccag aagatcgcga tgatccggct cgacgagcgt ctccgggccg gggcgttccg
     3661 ctcgcgcctg ctcctgtcgg tccacgacga gctccttctc gaggtcccgc gcgacgagct
     3721 cgaccggctc gtcccgctcg tccgcgagac gatggagagc gccctcgcgc tggacgtccc
     3781 cctgaccgtc gacgtcaagg tcggcgacga ctgggagtcg atgacgccgc tcacccgggc
     3841 cgacgcgatc gccgccgagg ccgccgaggc gccggccgaa ccgatcgccg ggtaggctct
     3901 cgcccgatgc ctgaattgcc cgaggtcgag acggtcgtcc gcgacctccg acccctgatc
     3961 accggcgcca cgatcgtcga cgcgacgacg ctctgggcgc ggacgctgcg cgggatgtcc
     4021 gccgaggcgt tcgccgacgg cgtcgccggc cggcggatcg agtcggtcgg gcggcgggcg
     4081 aagcagatcg tcatcgacct ctcgggcggc gcattcctga cgatccacct gaagatgacc
     4141 ggacagctct tcgtcgtgcc ccagggcgga ccggaagacc cgtacgtccg gctggtgctc
     4201 ttcctcgagg acggacggga gatccgcttc cgcgacatcc gcaagttcgg caaggtcggg
     4261 ctgtacgggg cggattcgtc ggtcgccgag gccgacccat tcgcgcggat cggcccggag
     4321 cccctcgccg acgcgttcac cgtggccgtg ctccgacggc ggatccgtga gcggaagggc
     4381 cgcctcaagc cgctcctcct cgaccagtcg ttcctcgccg gcatcggcaa catctatgcc
     4441 gacgaggcgc tctgggcggc ccgcctccac ccgctccgga ccgcgcggac gctccgaccc
     4501 gcggacgaac gacggctctg gctggagatc cggcggatcc tcgccgaggc ggtgatccgg
     4561 cgcggctcgt cgatcgacga ctacaccgca cccgacggcg acggcgagat gcaggaccac
     4621 ctcgcggtct accagcagac cggtgagccg tgcctgcgct gcgggcggcc gatccggcgg
     4681 atcgtcgtcg gcgcgcgggc aacccatttc tgctcgtggt gccagcgcct gccggccacg
     4741 gaccggtccg gagcgagggc gatcctccgc acggcgtcga cccccgaacg gccgggtcgg
     4801 cgctggtcgg agctgccggg cggggagggg agcgtcgggc tcgcccgggc
//
LOCUS       DATAIW010000318         6385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_127783, whole genome shotgun sequence.
ACCESSION   DATAIW010000318 DATAIW010000000
VERSION     DATAIW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127783"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VHM48_09800"
     CDS             complement(<1..490)
                     /locus_tag="VHM48_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755747.1"
                     /translation="MVESVASADVQNELGGPPRRMETGRPGRRARVDGLIVGFAALVL
                     FALWQGGRSTSPSPSVAPSAGTAGSDPVVALIDANGALATVDLNGRSVTLDAEPGTSF
                     GFPAWSPDGSRIAAVVAGADGSSISIFGAHPTDSGPSPAAPVVVYRSADVPPFYLYWT
                     PDG"
     gene            612..1478
                     /locus_tag="VHM48_09805"
     CDS             612..1478
                     /locus_tag="VHM48_09805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2755748.1"
                     /translation="MRQAHPRNQHRKRRAIGPALIAFVVGSLGFLAWGGPGFGKDSGR
                     PDIAGAMPVTSVAVVRPSLPPPAATLPPIVESRSGRPANGRPTVLLPDVEPIVVAGPG
                     PGSKAVFLGDSYTSGWNGTGLGAKGWPALVGAARGWKVVNLAIPGTGFMNPGWTSQPI
                     GSRIDAAARQKPDVVFVAGGHNDSRWSAATTAQAADRAIRRLHAALPDAVIVIVAPIW
                     QDGSPPMRCLVLRDALRRTARAIGAVFIDPLAERWFAGSSHRFIVADGFHPTNAGHRY
                     LADRIIADLSGI"
     gene            complement(1494..3665)
                     /locus_tag="VHM48_09810"
     CDS             complement(1494..3665)
                     /locus_tag="VHM48_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2755749.1"
                     /translation="MSATPDRTSHDGFRPEVEGLRGVAVLIVVLFHAGLAGVTGGFVG
                     VDVFFVISGFLITGLLLREHERTGRIAFLAFYARRARRLLPAAVVVLIVTLIAAMNLV
                     APLDRPSVGLDGAAAALSLGNIRFALASGDYFANVAAPSPFLHFWSLAVEEQFYLVWP
                     ALILIVARGVHARRRVVLALLGLVMASFVGNVVLTDLAANWAFYSLPTRAWELGLGGL
                     VAVVAGAGIVDRAPRGLASVFGWLGLAAILIATFSFDQAMAWPGSAALVPAVGAAVLV
                     ASGRGRLGPSLLLATAPLRFLGRISYSLYLVHWPILVLAPFALGGQVDELGRAGLVLL
                     SVLAATLSWAVIETPFRGPLPRLVSPPGRTVSVGLASILAVVVIAAAPSMGWGAAPAA
                     AAETTASALIDTSDASWPDDLVVPLPSTGPSHQPAPSPTSPTTNPAPSAKPAASPVPR
                     PVLPEDVAATGALSSDVRPALADARGDEERLRADGCLAFERVTVPPDCIYGVPAGAFT
                     VALVGDSHAAQWFPALDRLARHEGWRVVTFVKVACPFIDMPVRNTALKRDYRECADFN
                     EATIARLNAIRPDLTLVSMSRIAIHPLTTTDDTVAAKGAAVGRMLSRLSGRVAIIVDT
                     PYAGRDVPACLSANLADVEACAIPQAVAFTDHLGALEKAAAKAAGADRIDLTQHICIG
                     TPCPVVVNDRIVYRDIGHLTATFARSLAPALAAAIALILAR"
     gene            3848..4492
                     /locus_tag="VHM48_09815"
     CDS             3848..4492
                     /locus_tag="VHM48_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2755750.1"
                     /translation="MRATRPLPTRIAEIAVPQDEVSQATWRWAQRSLPGYLLAHSVRS
                     WCWGATIAAVEGWTFDPRILWTASLMHDLGLTRIPRNTMCFEIEGAEIARRFLERSGL
                     PAEDADRVAIAIILHMRSSVTLDDGVESVLLDRATGIDVRGDGYELIAGVRAAVVEVF
                     PRGPFDRHFLRAIEREVAIRPDCQSARLLNRTGLADWMARSPWAPDATAPDRRG"
     gene            4485..5462
                     /gene="pip"
                     /locus_tag="VHM48_09820"
     CDS             4485..5462
                     /gene="pip"
                     /locus_tag="VHM48_09820"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX2755751.1"
                     /translation="MAEVQMPEPYPSIEPYESGMLAVGDGQSIYWEASGNPHGKAAIA
                     LHGGPGSGSRPGRRRWFDPARYRIIQLDQRGCGRSTPHAGDWSTDLVTNTTHHLIADI
                     ERLREHLGIERWLVWGASWGVTLALAYAERHPERVTEMVLLSITMTRRADVQWLAHDT
                     GRFFPEEWARFRAGVPETDRDGDLVAAYDRLLNAHPDPAVRLQAARDWVAWEDAILSL
                     EEGYQVPNPRWADEQYMIGFARLVTHYFSHAAWLDDDELLRNASRLAGIPGVLVHGRL
                     DLSGPPDVAWQLARAWPGAELHFVPGGHTGDAEMSRLELEATDRFASRS"
     gene            complement(5470..>6385)
                     /locus_tag="VHM48_09825"
     CDS             complement(5470..>6385)
                     /locus_tag="VHM48_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2755752.1"
                     /translation="LGRIQRGEVGGVILFGANITTAGALRQLTATLRAAAAAGGRPRL
                     FIAVDQEGGSVKRIPWIPPTLSPPQMGRLASTSVARTQGAATGAALLDLGIDVDFAPV
                     ADVPASKASFMYLAGRTWSFDAARTAWLSNAFAQGLRMRGVVPTLKHFPGIGYATKNT
                     DSSVVKIWASRAQLSPGLAPYRRAVINDLPLIMLSNATYPAYDAVHAAGWSPAIVGTL
                     LRHDLGFTGVTITDSLDGTANARGISTRRLAAAAATAGTDMILTTGSETATQSVFAYL
                     VQQATAGAIPRATLQASYARILALKARL"
BASE COUNT          988 a         2181 c         2349 g          867 t
ORIGIN      
        1 gtccgtcggg cgtccagtag agatagaaag gcggaacgtc ggcgctccgg tagacgacga
       61 cgggggccgc cgggctcggg ccggagtcgg tcgggtgggc cccgaagatc gagatcgagg
      121 agccgtcggc gccggcgaca accgcggcga tgcgcgaccc gtccggcgac caggccggga
      181 acccgaagga tgtgccgggt tccgcatcga gcgtgaccga gcgcccgttc aaatcaacgg
      241 tggcgagggc tccgttggcg tcgatcaggg cgacgactgg atcggaaccg gccgttcccg
      301 cactcggcgc gaccgatggc gatggcgacg tcgaccggcc gccctgccac agcgcgaaca
      361 ggacaagggc ggcgaacccg acgatgagtc cgtcgacgcg ggctcgtcgt cccggtcggc
      421 ccgtttccat gcggcgcggc ggcccaccga gctcgttctg aacgtcggcg ctcgccacgg
      481 actcgaccat ctacgaacga tacgcgggcg gccggttcgt ggatgtcgat gcgttcgccc
      541 gtcgtcgagg gtcgtcgcgc ccgcgcgcta cactgcctcg acgcaatccg cgaccaccag
      601 acgaggatcg ggtgcgccag gctcatccgc gcaaccagca ccggaaacgg cgagccatcg
      661 ggccggcctt gatcgcgttc gtggtcgggt cgctcgggtt cctggcgtgg ggcggacccg
      721 ggttcggaaa ggactccggg cgaccggaca tcgcgggcgc catgcccgtg acgtcggtcg
      781 ccgtggtccg accgtcgctt ccgcctccgg cggcaaccct tccgccgatc gtcgagtcgc
      841 gctcaggccg acctgcgaac ggccgaccca cggtcctgct gccggacgtc gagccgatcg
      901 tcgtggccgg ccccggaccc gggtcgaagg ccgtcttcct cggcgattcc tacacgtccg
      961 gctggaacgg taccggcctc ggcgccaagg gctggcccgc actcgtcggt gcggctcggg
     1021 gctggaaggt cgtgaacctc gcgatccccg gaaccggctt catgaacccc ggctggacgt
     1081 ctcagcccat cggctcccgg atcgacgcgg cggctcggca gaagcccgac gtcgtgttcg
     1141 tggcgggcgg tcacaacgac tcgcgctggt ctgccgcgac gacggcccag gccgcggacc
     1201 gggcgatccg tcgtctgcac gccgctctgc cggacgcggt gatcgtcatc gtggccccga
     1261 tctggcagga cggcagcccg cccatgcgct gcctcgtcct gcgcgacgca ctgcggcgaa
     1321 cggcccgggc gatcggcgcg gtcttcatcg accccctcgc cgaacgttgg ttcgcgggtt
     1381 ccagccaccg gttcatcgtg gcggacgggt tccacccgac caacgcgggc caccgctacc
     1441 tcgccgatcg gattatcgcc gacctcagcg ggatctgaac cggatccgac cactcagcgg
     1501 gcgaggatca gagcgatcgc cgcggcgagc gctggagcga gggatcgggc gaaggtcgca
     1561 gtgagatgac cgatgtcgcg atagacgatc cggtcattga ccacgaccgg gcacggggtt
     1621 ccgatgcaga tgtgctgggt gaggtcgatg cggtccgccc cggcagcttt cgcggcggcc
     1681 ttctcgagcg ccccgaggtg gtcggtgaac gcgacggcct gggggatcgc gcaagcctcg
     1741 acatcggcca gatttgccga caggcaggcc ggaacgtcgc gcccggcgta cggcgtgtcg
     1801 acgatgatcg cgacccggcc gctgagccgg ctcagcatcc ggccgacggc cgcacccttc
     1861 gccgcgacgg tgtcgtcggt ggtcgtcaat ggatggatgg cgatccggct catcgaaacg
     1921 agcgtgaggt cgggccggat cgcgttgagc cgggcgatcg ttgcctcgtt gaagtcggcg
     1981 cactcgcggt agtcgcgctt gagggccgtg ttccggacag gcatgtcgat gaacgggcag
     2041 gcgaccttga cgaaggtgac cacgcgccag ccctcgtgcc tggcgagccg atcgagcgcc
     2101 gggaaccact gggcggcgtg cgaatcgccg accagggcga cggtgaacgc gccggccggg
     2161 acgccgtaga tgcaatcggg gggaaccgtg acccgttcga aggcgaggca cccgtcggcg
     2221 cggaggcgct cttcgtcgcc acgggcatcg gcaagggcgg gccggacgtc cgaggacagg
     2281 gcgccagttg cggcgacgtc ctccggcagg accgggcgcg ggaccgggct ggcagccggc
     2341 ttggccgagg gagcggggtt cgtcgtcggc gaggtgggcg acggtgccgg ctggtgggac
     2401 gggcccgttg acggcagcgg gacgacgagg tcgtcgggcc acgacgcatc actcgtgtcg
     2461 atcaacgcgg atgccgttgt ctcggcggcg gcggccgggg ctgcgcccca gcccatcgac
     2521 ggtgccgcgg cgatcacgac gaccgcgagg atcgatgcca ggccgaccga gacggtccgt
     2581 ccgggcggcg agacgagacg cggcaacggc ccccggaacg gggtctcgat gacggcccac
     2641 gagagagttg cggcgagcac ggacaggagg acgaggccgg ctcgaccgag ctcgtcgacc
     2701 tgaccgccga gagcgaacgg agcgagcacc aggatcggcc agtggacgag gtacagcgag
     2761 tagctgatcc gcccgaggaa cctgagtggc gccgtggcga gaaggagact cgggccgagg
     2821 cgaccacggc cggaagcgac caggaccgcc gccccgacgg cgggcacgag tgccgcgctc
     2881 cctggccagg ccatggcctg atcgaacgag aacgtggcga tgaggatcgc ggcgaggccg
     2941 agccagccga agacgctcgc caggccgcgg ggcgcccggt cgacgatgcc ggcgccggcg
     3001 acgacggcga cgaggccgcc cagccccagt tcccaggcac gggtcgggag cgagtagaac
     3061 gcccagttcg cggcgagatc ggtgagcacg acgttaccga cgaacgatgc catcaccagc
     3121 ccgaggaggg cgaggacgac ccgtcgccgg gcgtggacgc cgcgggccac gatcaggatc
     3181 agggcgggcc agaccaggta gaactgttcc tcgaccgcga ggctccagaa atggaggaac
     3241 ggcgatgggg ccgcgacgtt cgcgaagtag tccccgctcg ccagcgcgaa ccggatgttg
     3301 ccgagcgaga gcgctgccgc ggcaccgtcg agcccgaccg acggacggtc gagcggcgcg
     3361 accaggttca tcgccgcgat caacgtcacg atcaggacga cgaccgccgc cggcaggagt
     3421 cgacgggccc gtcgtgcgta gaacgcaagg aacgcgatcc gcccggtccg ctcgtgctca
     3481 cggagcagga gcccggtgat cagaaagccg ctgatgacga agaagacgtc gacaccgacg
     3541 aacccgccgg tgacgccggc cagtccggca tggaagagga cgacgatcag gaccgcgacg
     3601 ccgcggaggc cttcgacttc ggggcgaaat ccgtcgtgac tggtgcgatc cggtgttgcg
     3661 gacacgatcg ggactcctgc ggagcagcca tcctcgcgag caaccctcgt cggatggtcg
     3721 atgcagtggt gacgccacgt gcccgggcct gtgactgtca agaaactgac agctctccac
     3781 cgcaacacag cctcgacccg gggcgtcgcg gccaccgccg cgggcccggc tcggtacgat
     3841 tgcgccgatg cgcgccacgc gaccccttcc gacccggatc gccgagatcg ccgtgcccca
     3901 ggacgaggtg tcgcaggcca cctggcgatg ggctcagcgc tcgctgccgg gctacctgct
     3961 cgcgcactcg gtccggtcct ggtgctgggg tgcgacgatc gcggcggtgg agggctggac
     4021 gttcgatccg cgaatcctct ggaccgcgtc cctgatgcac gacctcggtc tcacccggat
     4081 cccgcgcaac acgatgtgct tcgagatcga gggggccgag atcgcccgcc gcttcctcga
     4141 gcgcagcggg ctgccggccg aggacgccga ccgtgtcgcg atcgcgatca tcctccacat
     4201 gcggtcgtcc gtgaccctcg acgacggcgt cgagagcgtc ctcctcgatc gggcgaccgg
     4261 gatcgacgtt cgcggcgatg gctacgaact gatcgccggg gttcgggccg ctgtcgtcga
     4321 ggtgtttccg cgcggaccgt tcgaccgcca tttcctgcgg gcgatcgagc gggaagtcgc
     4381 gatccgtccg gattgccaga gtgcccgtct gctcaatcgg actggcctgg cggactggat
     4441 ggcacggtcg ccatgggcac cggacgccac cgcaccggat cgccgtggct gaggtgcaga
     4501 tgcccgagcc gtatccgtcc atcgagccgt acgagagcgg catgctcgcc gtcggcgacg
     4561 gccagtcgat ctactgggag gcctcaggca acccgcacgg gaaggccgcg atcgcgctcc
     4621 acggcggccc aggcagcggc agccggcccg gccggcgccg ctggttcgat cccgcccgct
     4681 accggatcat ccagctcgac cagcgcggct gcggccggag cacgccgcac gccggcgact
     4741 ggtccacgga tctcgtgacg aacacgacgc atcacctgat cgccgacatc gagcggctgc
     4801 gggagcacct cgggatcgag cgctggctcg tctggggcgc gtcgtggggc gtgacgctcg
     4861 ctctcgccta tgccgagcgg cacccggagc gggtgaccga gatggtcctg ctctcgatca
     4921 cgatgacccg ccgcgccgac gtccagtggc tggcccacga cacgggcagg ttctttcccg
     4981 aggagtgggc ccggttccga gcgggtgtcc ccgagaccga ccgggacgga gacctcgtcg
     5041 cggcatacga ccgcctgttg aacgcccacc cggacccggc cgtgcggctg caggctgccc
     5101 gggactgggt ggcctgggag gacgcgatcc tgtcgctcga ggagggctac caggtcccca
     5161 acccgcgctg ggcggacgag cagtacatga tcggcttcgc gcgactcgtc acccactact
     5221 tctcgcacgc cgcctggctg gacgatgacg agcttctccg caacgcctcc cggctggccg
     5281 ggatcccggg cgtcctcgtc catggccgcc tcgatctatc cggtccgccg gacgtcgcct
     5341 ggcagctcgc ccgcgcgtgg cccggcgcgg agctccactt cgtcccgggc ggccacactg
     5401 gtgatgccga gatgtcgcgt ctcgagctcg aggcgaccga tcggtttgcc tcccgctcct
     5461 gacgcaagct cagaggcggg ccttgagggc gaggatccgg gcgtaggacg cctggagcgt
     5521 tgccctgggg atcgcgccgg cggtcgcctg ctggacgagg taggcaaaga cgctctgtgt
     5581 cgccgtctcc gatccggtcg tgaggatcat gtcggttccc gcggtggcgg cagcagcggc
     5641 gagccggcgg gtcgagatcc cccgggcgtt cgcggtaccg tcgagcgagt cggtgatcgt
     5701 cacgcccgtg aagccgaggt catggcgaag gagggttccc acgatcgccg gcgaccagcc
     5761 cgccgcgtga acggcgtcgt aggcgggata cgtggcgttc gacagcatga tcagcggaag
     5821 gtcgttgatg acggccctcc gatagggcgc cagccccggc gagagctggg cgcgtgacgc
     5881 ccagatcttc acgaccgacg agtccgtgtt cttcgtcgcg tagccgatcc cagggaagtg
     5941 cttgagcgtc gggaccaccc cgcgcatccg gaggccctgg gcgaacgcgt tcgacagcca
     6001 ggcggtacgg gcggcgtcga aggaccacgt ccggccggcc aggtacatga aggatgcctt
     6061 ggacgcgggg acatcggcca cgggcgcgaa gtcgacatcg atgccgaggt cgaggagggc
     6121 ggcgcccgtt gcggcgccct gcgtccgggc caccgacgtc gaggcgagcc ggcccatctg
     6181 cggtggcgac aacgtcggcg ggatccacgg gatccgcttg accgagccgc cttcctggtc
     6241 gacggcgatg aagagacgtg gccggccacc tgctgcggcc gccgcccgga gggtcgcggt
     6301 caactgccgc agggcgccgg cggtcgtgat gttggcgccg aagaggatga cgccgccgac
     6361 ctcgccgcgc tggatccggc cgagc
//
LOCUS       DATAIW010000319         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_49790, whole genome shotgun sequence.
ACCESSION   DATAIW010000319 DATAIW010000000
VERSION     DATAIW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49790"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..480)
                     /locus_tag="VHM48_09830"
     CDS             complement(241..480)
                     /locus_tag="VHM48_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755753.1"
                     /translation="MTGDRPGPSPSGGEPPSPAVEPRPWLERIGLAAIAMVMGAMLTV
                     MAIVSWTGGEWILAAMSAAGAVLTVGVGLVTLIRG"
     gene            complement(477..1982)
                     /locus_tag="VHM48_09835"
     CDS             complement(477..1982)
                     /locus_tag="VHM48_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2755754.1"
                     /translation="MSAVPRSVARPKPLGRTIGHVGLALTVAFAGIALGAGYWQVIRS
                     PDLSRAPDNPAVIAAARNVVRGSIVDRDGKVLASNKRSGDTGEPYRVYADRAFSTVIG
                     YASTDFGTAGLERSWGAELTGVSNRDPIGDVLRKFRSDPYDPQKLTLGISSVLQRAAI
                     AGLGDDRGAVVMLDPTTGEILALASTPTYDTSAIANPKTSRDAFAAVRDSAAKPLLTR
                     ATQGQYVPGSVFKIVTAIAGLGSGRITAATTFPEQPKAEQDGLVVGGFRIHEHAGVPS
                     RSFNLGAATEWSSNIWYALAGMRTGGDNLVKFAERMGFGAPLGFDLPTAPSQVTSGGG
                     GAPGGFSDETELANASYGQAETLVTPLQMALVAATVANGGTLMKPHLVIATTGRDGTR
                     TVSPEQMGVVIGPEEAAAIAAAMQRAVEGSIGQQFTSGAKVPGVPTAGKSGTAELGGS
                     GAPHSWFIGFAPVDHPRVAIAVIVERGGRGGARAAPLAGDLLALYFKTFGR"
     gene            complement(1979..>2356)
                     /locus_tag="VHM48_09840"
     CDS             complement(1979..>2356)
                     /locus_tag="VHM48_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2755755.1"
                     /translation="GLFLVVVERGLRIAASAADEFRSLLAAGLALVIGTQALIIAAGN
                     LKLIPLTGITLPFISYGGSSLLVNGIIVGLLLALSDRGVEPPPPPKPRSRLARMTGRV
                     TRLETPAAARAPLPNPPARTMER"
BASE COUNT          338 a          818 c          873 g          327 t
ORIGIN      
        1 tgcttgccga cgatcactcg ctcgacgttg gcgaggacct tctctccggt ctgctcaagg
       61 gtcgtcatca ggctcctcgc aggaatcgcg gactgagcgt ccggcgatgg ggggtttgca
      121 cgatagtacg tccggcaggc gtgagaggtt cgcttgtgcc ctctgacggg caccatgttc
      181 gcggtgtggc gtctcgcacg cgtcgcgggc ggcgatggcc cggcgcgtca ccgctcgctg
      241 tcagccgcgg atcagcgtga ccaggccgac gccgacggtc aggaccgcgc cagccgcgct
      301 catcgcggcg aggatccatt cgccgcccgt ccacgagacg atcgccatga ccgtcagcat
      361 ggcgcccatc accatcgcga tcgcggcgag cccgatccgt tcgagccacg ggcgcggctc
      421 caccgccggg gaaggcggct cgcccccgga cggcgacggg cccggccgat cgcccgtcac
      481 cggccgaatg tcttgaagta caacgccagc agatcgccgg cgaggggggc cgctcgggcg
      541 ccgccgcgcc cgccacgttc gacgatgacc gcgatcgcga cccgcgggtg gtcgaccggg
      601 gcgaagccga tgaaccacga gtgcggcgcc ccgctcccac cgagctcggc ggtgcccgac
      661 ttgccggccg tcggcacgcc cggcaccttg gccccgctcg tgaactgctg gccgatcgag
      721 ccttccaccg cccgctgcat cgccgccgcg atcgccgcgg cttcctcggg gccgatcacc
      781 acgcccatct gctccgggga gaccgtccgg gtcccgtccc ggcccgtcgt cgcgatcacg
      841 agatgcggct tcatcagggt gccgccattg gcgaccgtcg cggcgacgag cgccatctgg
      901 agcggcgtga cgagcgtttc cgcctggccg taggaggcgt tggcgagctc ggtctcgtcg
      961 ctgaagccgc cgggagcgcc accgccgccg ctcgtcacct gcgacggggc cgtcggcagg
     1021 tcgaagccga gcggcgcccc gaagcccatc cgttcggcga acttgacgag gttgtcgcca
     1081 ccggtccgca ttccggcgag ggcgtaccag atgttcgacg accactcggt ggccgccccg
     1141 aggttgaacg agcgcgatgg caccccggcg tgctcatgga tccggaaccc gcccacgacc
     1201 agaccgtcct gctcagcctt cggttgctcg ggaaaggtcg tcgcggccgt gatccgtccg
     1261 gaccccagac cggcgatcgc ggtcacgatc ttgaacacgg agcccggcac gtactggccc
     1321 tgggtcgccc gggtcaggag cggcttcgcg gcactgtcgc ggaccgcggc gaacgcgtcg
     1381 cgggacgtct tcgggttggc gatcgcgctc gtgtcgtagg tcggcgtcga ggcgagggcg
     1441 aggatctcgc cggtcgtcgg atcgagcatc acgacggcgc cgcgatcgtc gccgagaccg
     1501 gcgatcgcgg cccgctggag gaccgacgag atgccgaggg tcagcttctg cgggtcgtag
     1561 gggtcggagc ggaacttgcg caggacgtcg ccgatgggat cgcggttcga gactcccgtg
     1621 agctcggcgc cccaggagcg ctccagcccg gccgtgccga agtcggtcga ggcgtagccg
     1681 atcacggtgc tgaacgcccg gtcggcgtag acccgatacg gctcaccggt gtcgccgctg
     1741 cgcttgttgg aggcaaggac cttgccgtcg cgatcgacga tcgagccgcg gacgacgttg
     1801 cgggcggcgg cgatcaccgc cgggttgtca ggagcgcggg acaggtcggg cgaccggatg
     1861 acctgccagt agccggcacc aagggcgatc ccggcgaacg cgacggtcag ggccaggccg
     1921 acgtggccga tcgtccggcc caggggcttc ggtcgagcga ccgagcgggg cacggcgctc
     1981 atcgctccat cgtccgggcc gggggattcg ggagaggggc gcgagccgct gccggagtct
     2041 cgaggcgggt cacccggccg gtcatgcggg ccagccggct gcgcggcttc ggtggtggtg
     2101 gcggctcgac gccacggtcg gacagggcga ggaggagccc gacgatgatc ccgttcacga
     2161 ggagggacga gccgccgtag ctgatgaacg gaagcgtgat cccggtcaac gggatcagct
     2221 tgaggttgcc cgcagcgatg atcagcgcct gggtcccgat gacgagggcg agcccggcgg
     2281 cgagcaggga gcggaactca tccgcggccg aggcggcgat ccggaggccg cgctcgacca
     2341 cgacgaggaa cagccc
//
LOCUS       DATAIW010000320         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_19889, whole genome shotgun sequence.
ACCESSION   DATAIW010000320 DATAIW010000000
VERSION     DATAIW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19889"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            670..1008
                     /locus_tag="VHM48_09845"
     CDS             670..1008
                     /locus_tag="VHM48_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX2755756.1"
                     /translation="MTYVDRTLTCVDCGVEFIHSAADQEYYVQKGFASDPKRCASCRA
                     SRRATREGGTDVRDIGGPRGYERGDEQVAREYFAVVCSSCGNQAQVPFKPRMDRPVYC
                     SDCFRQVKPD"
     gene            complement(1604..2317)
                     /locus_tag="VHM48_09850"
     CDS             complement(1604..2317)
                     /locus_tag="VHM48_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX2755757.1"
                     /translation="MPESGPIALVGSGEFLPSMNDIDAGLLAATGHRRPRVVILPTAS
                     WPDGEDVFRRWAAMGIEHFTALGAEVEPVFVRDRFDADDDAHVQAIGEADLIYLSGGK
                     PGHLTGALIGSPVGEALLAAHRRGATLAGCSAGAMTLAARHWDTRKRRLFWPLRWRDG
                     LGLVPGATVIPHYDAFPEPMSAMVVLQAPRGLVTLGIDEETALVGRDGSWQVQGHGRV
                     TVWRGRHRQRFRAGDVFRL"
     gene            2359..3978
                     /locus_tag="VHM48_09855"
     CDS             2359..3978
                     /locus_tag="VHM48_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755758.1"
                     /translation="MTRAQRWTLLAAIVASGIVFLDSTVVNLALKRIGQELPTTTSLG
                     VLEAQAYVGSGYLAVLAALLIVAGALADRYGRRRIFIIGLASFGTISVFCGLAPTIEI
                     LIVARLLQGAAGALLVPGSLAIITAAFDGAARARAFGLWAASTSALTLLGPILGGLLV
                     DNISWRVAFLINVPLVAIALWSAIRHVVESRDPDNDGHFDWLGSVVGAVAVGGLAFGA
                     IRGQESQWADNRAWLALGIGAVSLVLFPILMAVRPRPLVPLSLFRNREFATINLATFL
                     IYGALYVSFTYTALLYQGTLGYSATGAAIIGLPSGILLATLSARVGGLTARFGSRIFL
                     VIGPLIVAAGQLWLARIPATSAPWLADLGRPASLIPPIDALIDVLPSAIAFGAGMAMV
                     VAPLTSTLMSSIPARNSGLGSAINNALSRVGQPLLGAVIFVAITASFYSALAASVPGL
                     DPADPAIRSMIVPLNPPKAGTPAAEVAAAKVASVDAFHLAALVSAGLMVAGSATSWLG
                     LRPATGRAQAMGSGATRSEGPHVDSIATGPG"
BASE COUNT          549 a         1457 c         1389 g          633 t
ORIGIN      
        1 gcgctgggcg gccgcctgat cgccgaggtc ggtgtggcgc gcgccgaggt ggacgtctgc
       61 ggtgtggagg agtcggagca tccggtcggg ctggcctcct gtgccgtcgc ggctgggcga
      121 ccagacgaga tggctcgccc agtccaggga ccgtcctcat ccccggcgcc ggtaccgagc
      181 aaggccgccc ccgacgtccc gctcgccgcc tcgactgttc gacgcattgt gccagacgcg
      241 ggggcaatcg cgacactggc cggatcgccg tctccctgat cgccctatga ccgaccgcgg
      301 tctgcgccgc gggcccgggt gcgcacggaa cgcgccaaag cgcgaacggc gaccagcgaa
      361 cgggcgggcg atcggaacat cgtctccgac gctgatcagc gcttccggca cgccgagggg
      421 tccgtcacgc tcaagaactc gtcgcggctg gtgatgctgc cgtcccggct gttgcaacgc
      481 ccgttccggg cgatcggtgt atggtcgatc catcgatcaa cccgtgcgcc ccgagacagg
      541 gactccataa cggcacctgg atagcgcggc gccggcccct tccgacgcaa ccgcaccatc
      601 ccgtccgccg acatggccgc cccggtttcg cccgggacca gccaacgtgc cagcaggaga
      661 ggaggtttcg tgacctacgt cgaccgaacg ttgacctgcg tcgactgtgg ggtggagttc
      721 atccactcgg ccgctgacca ggagtactac gtccagaagg gcttcgcctc ggacccgaaa
      781 cggtgtgcca gctgccgcgc cagccggcgg gcgactcgcg agggaggcac cgacgtccgc
      841 gacatcggcg ggcctcgggg ctatgagcgc ggggacgagc aagtcgcccg agagtatttc
      901 gccgtcgtct gctcatcatg cggcaaccag gcgcaggtgc cgttcaagcc gcgcatggat
      961 cgcccggtct actgctccga ttgcttccgg caggtcaagc ccgactgacg ctcgtcctga
     1021 cccaccccgg tcgagctcgg cggtccagac ccgagttccc ggatcacctc gccagaacgc
     1081 cccggtcaat ccgaggcgtt cgcgattcag tggcacgatc gtcggctggc gcgccttcgc
     1141 ggccgatcgc ggcgttgccg cctgcgctcg accacgcatt gctcgacgaa cgcagtcgcg
     1201 ggcatgacgg aggcgttcgc cgggccgatc gccgcccgat ttcgccatac gccactgcca
     1261 tgcgcattcg gccgggccgg cctgctcgac cggcgagctc cacgctcggc ggatgcggtc
     1321 gtttcgccat ttgcgcgctc caagtgcgta tggcgacgat caacgtggct gatgcgcgcc
     1381 tgagtggcgt atggcaattt cgccggatcc aagccgggat cccgcgaccg ctcggcgacc
     1441 gcggtgggcg acgaacgccg caccgccgcc gatcgcaact gcgttcgtcg cgctggggcg
     1501 aggatggcaa ggcgcctgcg ctcaccaggg tccgaggcgg agctccgcga cgccatcggc
     1561 ccgaggtggc aacgtcgcca ccgtcgttcc agcgcgacag cccttaaagt cggaacacgt
     1621 ctccggcgcg gaaccgctgg cggtgtcgcc cgcgccagac ggtcacccga ccgtggccct
     1681 ggacctgcca cgagccgtca cggccgacga gcgcggtctc ctcgtcgatg ccgagcgtga
     1741 ccagtccgcg tggcgcctgg aggacgacca tcgccgacat cggctcgggg aatgcgtcgt
     1801 agtgcgggat gacggtcgcg cccggcacca gcccgagccc gtcccgccag cgcagcggcc
     1861 agaagagccg tcgcttccgc gtgtcccagt gccgcgcggc aagggtcatc gccccggccg
     1921 agcaaccggc gagcgtcgcc ccgcggcgat gcgcggccag gagcgcctcg ccgaccggtg
     1981 agccaatgag ggcgcccgtc agatggccag gcttgccgcc cgacaggtag atcaggtcgg
     2041 cctcgccgat cgcctggacg tgggcgtcgt cgtcggcgtc gaagcggtcg cgaacgaaca
     2101 cgggttcgac ctcagcgccg agcgccgtga agtgctcgat gcccatcgcg gcccagcgcc
     2161 ggaagacgtc ctcgccgtcc ggccaggacg cggttggcag gatcacgacg cgcggccggc
     2221 ggtggcccgt ggccgcgagc aggccggcgt cgatgtcgtt catcgaaggc aggaactcgc
     2281 cggatccgac gagggcgatc ggtccagatt ccggcatgcg cgtgagcata tccgcggctg
     2341 tgcgacactg cccgccacat gacgcgcgcc caacgctgga cgctgctggc ggcgatcgtc
     2401 gcctccggca tcgtcttcct cgacagcacc gtggtgaacc tcgcgctcaa gcgaatcggc
     2461 caggagttgc cgaccacgac gtcgctcggt gtgctcgagg cccaggcgta cgtcggcagc
     2521 ggctacctcg ccgtcctggc cgccctgctg atcgtggccg gtgccctcgc cgaccgctac
     2581 ggccggcgcc ggatcttcat catcgggctg gcgtcgttcg ggaccatctc ggtgttctgc
     2641 gggctcgctc cgacgatcga gatcctgatc gtcgcccggc tgctccaggg cgccgcgggt
     2701 gcgctcctcg tgccgggctc gctggcgatc atcacagccg cattcgacgg cgcggctcgg
     2761 gctcgggcct tcgggctgtg ggccgcgtcg acgtcggcgc tgacgctgct cgggccgatc
     2821 ctcggcggtc tcctcgtcga caacatctcg tggcgggtcg cattcctgat caatgtcccg
     2881 ctcgtggcga tcgccctgtg gtcagcgatc cgccacgtcg tcgaatcgcg cgaccccgac
     2941 aacgacggcc acttcgactg gctcggctcg gttgtcggtg ccgtggcggt cggcgggctc
     3001 gcattcggcg cgatccgcgg ccaggaatcg caatgggccg acaaccgggc atggctcgca
     3061 ctcggcatcg gggcggtgag cctggtgctg ttcccgatcc tgatggcggt ccgaccgcgg
     3121 ccgctcgtcc cgctttcgct gtttcgcaat cgcgagttcg ccacgatcaa tctcgccacg
     3181 ttcctgatct acggcgccct gtacgtgtcg ttcacttaca ccgccctgct ctaccaggga
     3241 acgctcggct acagcgcgac cggagccgcg atcatcggcc tgccatccgg catcctgctc
     3301 gcgaccctgt cggcccgcgt cggcggcctg accgcccggt tcggttcgcg gatcttcctc
     3361 gtcatcgggc cgctgatcgt cgccgccggg cagctctggc ttgcccggat cccggcgacc
     3421 tcggcaccgt ggctcgccga tctcggccgg ccggcgtcgc tgatcccccc gatcgacgcc
     3481 ctgattgacg tcctgccctc cgcgatcgcc ttcggggccg gaatggcgat ggtcgtggcg
     3541 ccgctgacga gcaccctgat gagctcgatc ccggcgcgga actcgggtct cggatcggcg
     3601 atcaacaacg ccctgtcgcg ggtcggccag ccgttgctcg gagccgtgat cttcgtcgcg
     3661 atcacggcgt cgttctactc tgcgctcgcg gcgagcgtgc cgggcctcga tccggccgat
     3721 ccggcgatcc gcagcatgat cgtgccgctc aacccgccga aggccggaac tccggcggcc
     3781 gaagtcgcgg cggcgaaggt cgcctcggtc gatgccttcc acctcgccgc gctggtctcg
     3841 gccgggttga tggtcgcggg ctcggcgacg tcatggctgg ggttgcggcc ggcgaccgga
     3901 cgagcacagg cgatgggatc cggcgcgacc cggtcagagg gaccgcacgt ggattcgatc
     3961 gccaccgggc cgggctgagc ggcgatcgga atcggctgaa ccgagcgggg cgcgcccgac
     4021 gccgcggc
//
LOCUS       DATAIW010000321         6640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_127958, whole genome shotgun sequence.
ACCESSION   DATAIW010000321 DATAIW010000000
VERSION     DATAIW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127958"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VHM48_09860"
     CDS             <1..351
                     /locus_tag="VHM48_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755759.1"
                     /translation="KTFWIELARRRGARLLAVEVILSDDDLHRDRLAGRVRAFAIAEP
                     TWETVTLRREEWVAWPFAPLVIDSIEPLDVNVARVLEAARTIIAEQPDRSVADRAASE
                     DPGRNETPEGTPGP"
     gene            complement(533..1114)
                     /locus_tag="VHM48_09865"
     CDS             complement(533..1114)
                     /locus_tag="VHM48_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755760.1"
                     /translation="MATRLRFGGRGQGADGAAVVWSVAEGSRGRRWREVRNAGHAVAS
                     SLLLETDPAGQFAHLELSTPSGLLTLHPEGDGTLHGHAIVSDGIEHVAGLPWDADGVV
                     MLEGSTVCRAAAIGVLTGALEPFASKAHLAVVIPPTLWLEVKPIRIERIDATTWRFDA
                     EAPFAVDRHGLPLLHGGETWPLERDEDPAERHR"
     gene            complement(1122..2492)
                     /gene="hflX"
                     /locus_tag="VHM48_09870"
     CDS             complement(1122..2492)
                     /gene="hflX"
                     /locus_tag="VHM48_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2755761.1"
                     /translation="MPKPSAIDLTPPVEKAFLLAVDTGDDAGWTAVESLSELANLATT
                     AGANVVGAEWQNRRHVDPNWYVGKGKAEELTQAKSETGFDVLVTDDELSPSQQKALES
                     LLNVKVIDRSRLILDIFALHAQTHEGRLQVELAQLEYQLPRLTKLWTHLSRTGGGIGT
                     RGPGESQLETDRRLIRTRISKMKERVEQVRQQRQTAARGRDRRMMATVGIVGYTNAGK
                     STLLNTLVGSEVAKAEDKLFATLDPTSRQVKLGDGQTAIVTDTVGFIHKLPHQLVDAF
                     RATLEEVNRADVLIEVVDASDPHLREHRATVQTVLDELGAGAKPRLVAFNKADLVEKT
                     ALDGGAPAPALAGSVLVSALTGFGMDTLRAGIAALLASMWVDVDVHVPYAAGELLARI
                     RERGTVELDYGERDVRVHGRAAPSMAGELVAVAERWQESLAGAGTGGDGTSGDGSGHD
                     HRTGGG"
     gene            complement(2629..3606)
                     /gene="miaA"
                     /locus_tag="VHM48_09875"
     CDS             complement(2629..3606)
                     /gene="miaA"
                     /locus_tag="VHM48_09875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX2755762.1"
                     /translation="MSVGARAGQPTAAPPLLVVAGATATGKTGLAIALAERLVGEGIV
                     AEVISADSRQVFRGLDIGTAKATLDERRGISHHGLDLVEPDQPFSLADFVAHADSALA
                     GIADRGGLAILAGGTGLYLRAIARGIPTDALPSDADVRARLEAEFLAVGLDPLVERLR
                     AVAPERAATIDTRNPRRVVRALEIAALDGGRGRLPEPRGYNGPVAWIGLSVDRSTHLK
                     WIATRARAQFDAGLVEEARALRERFSPELPAFSAIGYREAWAVLDGRLDREQAITEDA
                     RRNAAFAKRQRTWFRAEPDITWLDADGSPGELALVAARGLLDATRPAGR"
     gene            complement(3757..5415)
                     /gene="miaB"
                     /locus_tag="VHM48_09880"
     CDS             complement(3757..5415)
                     /gene="miaB"
                     /locus_tag="VHM48_09880"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2755763.1"
                     /translation="MTLTRPTGRTLPVLDLVAPSSVAPAAPPAAPPEADDARLAEYRP
                     DTPTLPRFHVWTLGCQMNRSDSEEMAGRLLAAGCAEASSMDTADLVVINTCAIREAAE
                     QKVIGRQGVLKQLKAANPGMRVVLTGCAVREAERDGLRRRFPAVDLFLRPDEEAELVD
                     RLGLASAQGVVGMSSAATTVVGRATVGAADRLPRARAAAVEVGAVARDSAISAWLPIV
                     YGCDKTCTYCIVPFSRGPERSRPFDDVVAEARALAAAGYREVTLLGQNVNSYGHDLPP
                     DARFADVETERRVGRRLDLQGRPDLAALIRAIDAIRTADGVPAMARLRFVTSHPWDLS
                     DRLIEAMADCPSMCAHLHLPVQSGADAVLHRMGRQYTIEHYLERLARIRDAVPGIAIS
                     TDVIVGFCGETDAQFRSTLRLIETVRYDQVFAAAYSERPGTPATRLADDVPADVKRAR
                     LNELLALQEGIGLERNRAWIGREVEVLVDSVTPPRSHDHEHESDGGPGDAAAAVPTGP
                     RVAGRSRQNKLVHLAGGPELVGRLVTVRINHAGPYALRGVITGV"
     gene            complement(5447..6355)
                     /locus_tag="VHM48_09885"
     CDS             complement(5447..6355)
                     /locus_tag="VHM48_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2755764.1"
                     /translation="MAEKRPARHGRRPRPSAATGGSTVDLHSHTLRSDGVLQPVELVR
                     AAAAAGIKRLAITDHDTLSAYRELILGRAMPAPIELIPGVEINALAKGIPLAEGELHI
                     LGFGMDHTDDAFEAALAKQRSARKIRFERTVARLREIGLPIDEHLTHIDLTRDDALGR
                     PTIARALIVAGHATSVEDAFNRLIGWGGPGYVAREGMGPREAIDAIRGAGGVPVLAHF
                     SEAPDQVPLLRELVALGLVGLEVFYISFSPETVTAVGAVAAELGLLATGGSDYHGDTS
                     TYAEAHEALHVPDSVGDRLHEAMADR"
     gene            complement(6358..>6640)
                     /locus_tag="VHM48_09890"
     CDS             complement(6358..>6640)
                     /locus_tag="VHM48_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX2755765.1"
                     /translation="VSAVVGTHTHVVTGDERTLPGGTAYQTDLGMTGPVWSVIGFDPA
                     TVLPRFINALPTRFAVGDGPVIFNAAQIDIDPATGRALAIERIQRLIEV"
BASE COUNT          906 a         2333 c         2450 g          951 t
ORIGIN      
        1 aagacgttct ggatcgagct ggcccgccgc cgcggcgcac ggctgctcgc cgtcgaggtc
       61 atcctgtccg acgacgatct ccaccgggac cgcctggcag gtcgggtccg cgcgttcgcg
      121 atcgccgagc cgacgtggga gacggtcacc ctgcgccgcg aggagtgggt cgcctggccg
      181 ttcgcgccgc tggtgatcga ctccatcgag ccgctggacg tcaacgtggc tcgagtgctc
      241 gaggcggccc ggacgatcat cgccgagcaa ccggatcgga gcgtggcgga tcgtgccgcg
      301 tccgaggatc ccgggcgcaa cgaaacgccc gaaggaactc cgggtccgta gcctccactt
      361 ccaatgcaag caccggaagc gtcgctttcg atctcgtggc cccttcgggc aagacctact
      421 gtagaccgcc ccatccggcc tgcctaggga cttgtccacg actttcgcgg atcggtcggc
      481 caattcggtc gggttatcca cgttccgtcc acgtcggggc tcaccaggcg cgtcaacggt
      541 gccgttcggc agggtcctca tcacgttcga ggggccaggt ctcgcccccg tgcaggagcg
      601 gcaggccgtg ccgatcgacc gcgaacggcg cctcggcgtc gaaccgccag gtcgtcgcat
      661 cgatccgctc gatccggatc ggcttgacct cgagccacag ggtcggcgga atgacgacgg
      721 cgagatgggc cttcgacgcg aacggctcga gcgcgcccgt gaggaccccg atcgcggctg
      781 cccggcagac ggtcgagccc tcgagcatca ccacgccgtc ggcgtcccac gggagccccg
      841 ccacgtgctc gatgccgtcg ctgacgatcg cgtggccgtg gagggttcca tcaccttccg
      901 gatggagggt gaggagaccg gacggggtcg agagctcgag gtgggcgaat tgcccggccg
      961 gatcggtctc gagcaggagc gacgaggcga ccgcatgacc ggcattgcga acctcccgcc
     1021 agcgccgacc gcggctgccc tccgccaccg accacacgac cgctgcgccg tccgcgccct
     1081 ggccgcggcc gccgaaccgc agacgcgtcg ccatgcccgg ctcagccgcc cccggtccgg
     1141 tgatcgtggc cggacccgtc gccgctggtt ccatcgccgc cggttccagc gccggcaagc
     1201 gactcctgcc agcgttcggc gaccgcgacg agttcaccgg ccatcgacgg cgccgcccgg
     1261 ccgtggaccc ggacatcgcg ctcgccgtag tcgagctcga ccgtgccccg ctcgcggatc
     1321 cgggcgagga gctcgcccgc ggcgtacggg acgtggacgt cgacatcgac ccacatcgag
     1381 gcgaggagcg cggcgatccc ggcccgcagc gtgtccatcc cgaagccggt cagggcgctg
     1441 acgaggaccg agcctgcgag ggcgggagcg ggcgctccgc catcgagcgc cgtcttctcg
     1501 acgaggtcgg ccttgttgaa ggccaccaat cggggcttgg cgccggcgcc gagctcgtcg
     1561 aggacggtct ggacggtcgc ccggtgctcg cgcagatgcg gatccgaggc gtcgacgacc
     1621 tcgatgagca cgtcggcccg gttgacctcc tcgagcgtcg cacggaacgc gtcgacgagc
     1681 tggtgcggga gcttgtggat gaagccgacc gtgtcggtga cgatcgcggt ctggccgtcg
     1741 ccgagcttga cctggcgcga ggtcgggtcg agcgtcgcga acagcttgtc ctcggccttg
     1801 gcgacctcgc tgccgacgag ggtgttgagg agggtcgact tgccggcatt cgtgtagccg
     1861 acgatgccga cggtcgccat catccgccgg tcgcggccac gggcggccgt ctgacgctgc
     1921 tggcggacct gctcgacccg ttccttcatc ttcgagatcc gggtccggat cagccgccgg
     1981 tcggtctcga gctggctctc gccgggtccg cgcgtcccga tcccgccgcc ggtccgcgac
     2041 aggtgggtcc acagcttcgt cagtcgcggc agctggtact cgagctgggc gagctcgacc
     2101 tggagacggc cttcgtgggt ctgggcgtgg agggcgaaga tgtcgaggat cagccggctg
     2161 cggtcgatga ccttgacgtt gagcaggctc tcgagcgcct tctgctggct cggtgacagc
     2221 tcgtcgtcgg tgacgaggac atcgaacccg gtctcgctct tcgcctgcgt cagctcctcg
     2281 gccttgccct tgccgacgta ccagttgggg tcgacgtggc ggcggttctg ccactcggcg
     2341 ccgacgacgt tggcgccggc ggtcgtcgcg aggttggcga gctcggacag cgactccacc
     2401 gcggtccagc cggcgtcatc gccggtgtcg accgcgagca ggaatgcctt ctcgacgggt
     2461 ggggtgagat cgatcgcgct gggcttgggc acggccgaag gatacgcggg cctcgcgctg
     2521 ctggcgaccg tggcgccgtc acggcggccg agccgcgccc ggcggccccc gcctggctcc
     2581 ggcggagcag ccggccgcct gagtcgggtg accgcaggcg gcggtccgtc agcggcccgc
     2641 gggcctcgtc gcgtcgagca atccccgcgc cgcgaccagg gcaagttcgc ccggtgaccc
     2701 gtcggcgtcc agccaggtga tgtcgggttc ggcgcggaac cacgtccgct gacgcttcgc
     2761 gaaggcggcg ttgcggcggg cgtcctccgt gatcgcctgc tcgcggtcga gccggccgtc
     2821 gaggacggcc caggcttcgc ggtagccgat cgccgagaag gccggcaatt ccgggctgaa
     2881 tcgttcgcgg agggcgcgcg cctcttcgac cagaccggca tcgaactggg cccgggcgcg
     2941 ggtggcgatc cacttcaggt gtgtcgagcg gtcgaccgag agcccgatcc aggcgaccgg
     3001 tccgttgtag cctcggggct ccgggagccg tccgcggccg ccgtcgaggg cggcgatctc
     3061 gagggccctg acgacgcgac gagggttgcg cgtgtcgatc gttgccgcgc gctccggggc
     3121 gaccgcccgc agccgctcga cgagtgggtc gagcccgacc gcgagaaact cggcttcgag
     3181 tcgcgcccgc acgtccgcgt cggacgggag cgcatcggtc gggattcccc gggcgatggc
     3241 ccgcaggtac aggcccgtgc cgccggcgag gatcgcgaga ccgccccgat cggcgatgcc
     3301 ggccagcgcg ctgtcggcgt gggcaacgaa gtcggccagg ctgaacggct ggtcgggttc
     3361 gacgaggtcg aggccgtgat gggaaatccc gcgccgttcg tccagcgtcg ccttggccgt
     3421 cccgatgtcg agaccgcgaa acacctggcg cgagtcggct gagatgacct cggcgacgat
     3481 cccctccccc accaagcgtt cagcgagcgc gatcgccagg ccggtcttgc cggtcgcggt
     3541 cgcgccggcg acgacgagga gcggcggggc cgcggtcggt tgccccgcgc gggcgccaac
     3601 agacacctgg cgaccctgcg ctggcgcggc cggttgcccc gcgcgggcgc caacagacac
     3661 ctggcgcccc tttcgaatag ccgaatactg cgaatctcga atcattcggc tattggaaac
     3721 ggcagcgcgg gccagttcgg cagcgcagct cggcgctcac acgccggtga tcacgccgcg
     3781 cagcgcgtac ggcccggcgt ggttgatccg gaccgtgacg aggcgaccga cgagctccgg
     3841 accaccggcg aggtggacca gcttgttctg gcggctgcgg ccggccacgc gcgggccggt
     3901 cggcacggcc gccgcggcgt cgcccgggcc gccgtcggac tcgtgctcat ggtcatggct
     3961 ccgtggcggc gtgacgctgt cgaccaggac ctcgacctcg cgcccgatcc aggcccggtt
     4021 gcgctcgagc ccgatcccct cctggagcgc cagcagctcg ttcagacgcg cccgcttgac
     4081 gtcggcgggg acatcgtcgg cgagacgcgt cgccggcgtt ccgggccgct ccgagtaggc
     4141 cgcggcgaac acctggtcgt agcggaccgt ttcgatgagg cggagcgtcg agcggaactg
     4201 cgcgtctgtc tcgccgcaga atccgacaat cacgtcagtc gagatcgcga tcccggggac
     4261 cgcgtcgcgg atgcgcgcca ggcgctcgag atagtgctcg atcgtgtact ggcgacccat
     4321 ccgatgcagg acggcgtcgg cgccggactg gaccggcagg tggagatggg cgcacatcga
     4381 tgggcagtcg gccatcgcct cgatcaaccg gtcgctgagg tcccacgggt gggaggtcac
     4441 gaaccggagc cgcgccatcg cgggcacacc atcggcggtc cggatcgcgt cgatcgcccg
     4501 gatcagggcg gccaggtccg gccggccctg gaggtcgagt cggcgcccga cccggcgctc
     4561 ggtctcgaca tcggcgaacc gagcgtcggg cggcaggtca tgaccgtacg agttgacgtt
     4621 ctggccgagg agggtgacct cgcgatagcc ggcggccgcg agcgcgcgcg cctctgcgac
     4681 gacgtcgtcg aacggtcggc tccgctcggg gccgcgactg aacgggacga tgcaataagt
     4741 gcaggtcttg tcgcagccgt agacgatcgg cagccaggcg ctgatcgcgg agtcgcgggc
     4801 gacggcgccg acctcgacgg ccgcggcgcg cgccctcggc agtcggtctg ccgcgccgac
     4861 cgtcgcccgg ccgaccaccg tcgttgctgc gctgctcatc ccgacgacgc cctgggccga
     4921 cgccagcccg agccgatcga cgagctcggc ctcctcgtcc gggcgcagga acaggtcgac
     4981 cgccgggaag cggcggcgga gcccgtcgcg ctcggcttcg cggaccgcac agccggtcag
     5041 gaccacgcgc atccccgggt tggcggcctt cagctgtttc agaaccccct gccggccgat
     5101 caccttctgc tcggcggcct cacggatcgc gcaggtgttg atgaccacga ggtccgcggt
     5161 gtccatcgac gacgcctcgg cgcaaccggc cgcaagcagc cggccggcca tctcctcgga
     5221 atcgctccgg ttcatctggc agccaagcgt ccagacgtgg aagcgcggca gggtcggcgt
     5281 gtccgggcgg tactcggcga gccgcgcgtc gtcggcctcg ggcggagcgg cgggcggagc
     5341 ggcgggcgcc accgacgacg gcgcgacgag gtcgagcacg ggcagggtgc ggccggtcgg
     5401 tcgggtgagg gtcatcgccc gcgcatggta tccggccggc ccgcgttcag cgatcggcca
     5461 tcgcctcgtg gagccggtcg ccgaccgaat cggggacgtg gagcgcctcg tgggcttcgg
     5521 cataggtcga ggtgtcgccg tgatagtccg acccgccggt cgcgagcagg cccagctccg
     5581 cggcaaccgc gccgacggcg gtcaccgtct cgggcgagaa cgagatgtag aagacctcca
     5641 gcccgacgag gcccagcgcg acgagctcac gcaggagcgg cacctggtcg ggcgcctcgg
     5701 aaaagtgggc gaggaccggg acgccgccgg cgccgcggat cgcgtcgatc gcctcccgcg
     5761 gccccatccc ttcccgggcg acgtatcccg ggccgcccca gccgatcagt cggttgaacg
     5821 cgtcctcgac gctcgtcgcg tgacccgcga cgatcagggc ccgcgcgatc gtcggtcggc
     5881 cgagggcgtc gtcgcgggtc aggtcgatat gggtgagatg ctcgtcgatc ggcagcccga
     5941 tctcgcgaag ccgggccacg gtccgctcga accggatctt gcgcgccgac cgctgcttgg
     6001 ccagggccgc ctcgaaggca tcgtcggtgt ggtccatccc gaagccgagg atgtggagct
     6061 cgccctccgc cagcgggatc cccttggcga gggcgttgat ctcgacgccg gggatcagct
     6121 cgatcggggc cggcatggcc cggccgagga tcagctcgcg gtacgccgag agcgtgtcgt
     6181 ggtcggtgat cgccaggcgc ttgatccccg ctgccgcagc cgcccggacc agctcgaccg
     6241 gttggaggac gccgtcggag cggagggtgt gcgagtggag atcgacggtc gaaccgcccg
     6301 tggcggcgga gggtcgcggg cggcgcccgt gccgggcagg gcgcttctcc gccaccctca
     6361 gacctcgatc aggcgctgaa ttcgctcgat ggcgagggcc cgcccggtgg cgggatcgat
     6421 gtcgatctgg gcggcgttga agatgaccgg accgtccccc accgcgaacc gggtcggcag
     6481 ggcgttgatg aagcgcggca ggacggtcgc cggatcgaag ccgatgacgc tccagaccgg
     6541 gccggtcatc ccgagatcgg tctggtaggc cgtcccgccc ggcagggtcc gctcgtcgcc
     6601 ggtgacgacg tgggtgtggg tcccgacgac ggcgctgacc
//
LOCUS       DATAIW010000322         4657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_127978, whole genome shotgun sequence.
ACCESSION   DATAIW010000322 DATAIW010000000
VERSION     DATAIW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127978"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHM48_09895"
     CDS             <1..367
                     /locus_tag="VHM48_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755766.1"
                     /translation="TSDKWHRLAFRPSDTMAAFASVHSKNDLTYLGPVKSGGKTYYKV
                     EFRSAIVNPVMIPASNLTETVLTSPKLTLLVDAAGRPIKGTAEIDGRGRVSGQLQEIV
                     IDLTVTFTKLGQAVSIKAP"
     gene            complement(473..2074)
                     /locus_tag="VHM48_09900"
     CDS             complement(473..2074)
                     /locus_tag="VHM48_09900"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2755767.1"
                     /translation="MSRRAAGPRSSERWPRARSGERIGRPTRGSGRRPWPRFPEANPL
                     AKAMMAAVLVFFPVMANVTRGLVQVESGALELMRSYAASEWTVLRKVRVPNALPYSFT
                     AMKIATTLSLIGAIVGEYFGGATSVLGRRIVESASALKFDVTWAARQEESVDKKRGFA
                     LTAAAAVVFAACGSGATPVPASRTPGSVAPTTAAVRLQLQWVPQAQFAGYFAAEKQGY
                     YKAENLTVAMVPGGPDVVPQVEGSKAEGPEFTISWVPKVLEAREGGSDLVDIAQVFQR
                     SGTLSVTWKDTGLDEPCKLAGKKVGTWGFGNEFEVTAGLLTCGLTPGLENNGDKTKQF
                     QQVTQAFDMTAFLSRDIDAAEAMIYNEFAQVLEAPDPATGTLYSKDQLNIINWNDYRV
                     AMLQDAIFARKAWLDKGNNKDVAVRFVRASLKGWIYCRDHPDDCVQYTTDAGSQLGTG
                     HQKWMLNEVNALIWPSELGVGTLDPVLWSQTVRVARSADIIKTDPSVDAYATDIVKAA
                     LAGITDDALGKGFTKGTVEVTAGGK"
     gene            complement(2013..2876)
                     /locus_tag="VHM48_09905"
     CDS             complement(2013..2876)
                     /locus_tag="VHM48_09905"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2755768.1"
                     /translation="MTTQNPPGPVVRISGVDKTFRRGDQVVTKALEGIDLEIAQGEFV
                     SLIGPSGCGKSTLLRIVGDLTSPSSGTVTVNGKPAERARRDRDYGMVFQAPVLFDWRT
                     VEDNVKLPLEILGQDAATRTKRAREMLELVELGDFLKHHPYQLSGGMQQRVAIARALA
                     FEPSILLMDEPFGALDEMTRERMNDEVLRIWEQTGTTILFVTHSIPEAVFLSSRVVVM
                     SPRPGRITNVIDIDVPRPRNEDTRETRRYFELVTEVREALRAGGGTLDDSEPAGGGPA
                     VERAMAEGAVG"
     gene            complement(2902..3057)
                     /locus_tag="VHM48_09910"
     CDS             complement(2902..3057)
                     /locus_tag="VHM48_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755769.1"
                     /translation="MAGRRRRALTWVLLVVAVLVVWEGLKFLGGTPWRAPGALPGPNN
                     PVLSAAG"
     gene            complement(3119..4459)
                     /locus_tag="VHM48_09915"
     CDS             complement(3119..4459)
                     /locus_tag="VHM48_09915"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2755770.1"
                     /translation="MAVETTAQPSMSTEEIVRLSRAHTFFSWSAQAAIDPIAIDRAEG
                     VYLYTPEGQRILDFNSQLMSVNIGHGDRRVIDAITAQATKLQYVQPAFATEPRARLGR
                     KLAEILPGDLDKVFFTLGGAEAIENAIKLARAFTGRHKILARYRAYHGATLGAMTLTG
                     DPRRWANEPALVGVVRYPDTHRWGEAEPRPAAEALQGLEEVIRYEGGHTIAAIFLETI
                     VGTNGILIPPDGYIQGVRELCDRHGILMVADEVMAGFGRTGKWFAIQHWDTVPDLLTM
                     AKGLTSSYLPLGAVAMNHRIAEHFDTNFYYGGLTYSSHAISCAAALANIAVYEEDGLI
                     ENAARLDGVMRRHHEDLAARHPSVGAHRNLGLFGILDLVRSRDPWTPLTPFNGTSDEM
                     KAIGKYFRENGLYTMIANNSIHTNPPLCITEEQLGEGFAIIDRALDIADQAVTG"
BASE COUNT          686 a         1586 c         1628 g          757 t
ORIGIN      
        1 cacgagcgac aagtggcatc gcctcgcctt ccgcccgtcg gacacgatgg ccgccttcgc
       61 ctcggtccac agcaagaacg acctgaccta cctcggtccg gtgaagtccg gcggcaagac
      121 ctactacaag gtcgagttcc gatccgcgat cgtgaacccg gtgatgattc ccgcgagcaa
      181 cctgaccgag accgttctga cctcgcccaa gctgaccctg ctggtcgatg cggcgggccg
      241 accgatcaag gggacggccg agatcgacgg tcgtggccgg gtgtcgggtc agctccagga
      301 gatcgtgatc gacctgacgg tcacattcac caagctcggc caggcggtct cgatcaaggc
      361 tccctgagag ggcggcgatc ccgccgggaa tcgatcggcc ggcccctcgc gggaccggcc
      421 gagtcgcgga cgtgatgggt cggcgcgggt gccgcgcccc cgcgccggtg agctacttgc
      481 cgccggcggt gacttcgacg gtccccttgg tgaagccctt gccgagggcg tcgtccgtga
      541 tgcccgcgag ggcagccttc acgatgtcgg tcgcgtaggc gtcgaccgac gggtcggtct
      601 tgatgatgtc ggcactcctg gcgacccgga cggtctgcga ccacaggacc ggatcgagcg
      661 tcccgacacc gagctccgac ggccagatga gggcgttgac ctcgttcagc atccacttct
      721 ggtggccggt tccgagctgg ctgcccgcgt ccgtcgtgta ctggacgcag tcgtccggat
      781 gatcgcgaca gtagatccag cccttcagcg aggcccggac gaaacgaacc gcgacgtcct
      841 tgttgttgcc cttgtcgagc cacgccttgc gggcgaagat cgcgtcctgg agcatcgcga
      901 cgcggtagtc gttccagttg atgatgttga gctggtcctt ggaatagagc gtacccgtgg
      961 ccggatcggg cgcctcgagg acctgcgcga actcgttgta gatcatcgcc tcggccgcgt
     1021 cgatgtcgcg ggagaggaag gcggtcatgt cgaacgcctg ggtgacctgc tggaactgct
     1081 tggtcttgtc gccattgttc tcgaggccgg gcgtcaggcc gcaggtgagc aaaccggccg
     1141 tgacctcgaa ctcgttgccg aagccccacg tgccgacctt cttgccggcg agcttgcacg
     1201 gttcgtcgag accggtgtcc ttccacgtca ccgacagggt gccggagcgc tggaagacct
     1261 gggcgatgtc gacgaggtcc gagccgccct cgcgagcttc gaggaccttc ggcacccagc
     1321 tgatcgtgaa ctcaggcccc tcggccttcg agccctcgac ctggggcacg acgtccggtc
     1381 cgccggggac catcgcgacg gtcaggttct cggccttgta gtagccctgc ttctcggccg
     1441 cgaaatagcc ggcgaactgg gcctgcggca cccactggag ctggagcctg accgccgccg
     1501 tcgtcggggc gacactgccc ggcgtccgtg acgccggcac cggggtggcc ccgctgccac
     1561 acgcggcgaa gacgacggcc gccgccgcgg tcagcgcgaa accgcgcttc ttgtccacgg
     1621 actcctcctg cctcgccgcc caggtcacgt cgaacttcag ggcgctcgca ctctcgacga
     1681 tccgccgccc gaggaccgac gtcgccccgc cgaaatactc gccgacgatc gccccgatca
     1741 ggctcagcgt ggtcgcgatc ttcatcgccg tgaaggagta cggcaacgcg ttcggaacac
     1801 ggaccttgcg cagaaccgtc cactcgctcg cggcgtacga tcgcatcagc tcgagcgcgc
     1861 ccgactcgac ctggaccagg ccgcgggtga cgttggccat caccgggaag aagacgagga
     1921 ccgcggccat catcgccttg gccagcgggt ttgcctcggg gaaccgcggc cacggccggc
     1981 ggccggatcc acgagtgggg cgaccgatgc gctcacccga ccgcgccctc ggccatcgct
     2041 cgctcgaccg cgggcccgcc gcccgccggc tcactgtcgt cgagggtgcc gccgccggcg
     2101 cggagcgcct cgcggacctc ggtcacgagc tcgaagtagc gtcgcgtctc gcgcgtgtcc
     2161 tcgttgcgcg gccgcggcac gtcgatgtcg atgacgttgg tgatccggcc gggtcggggg
     2221 ctcatgacca cgacacgcga cgacaggaac acggcctccg ggatcgaatg ggtcacgaac
     2281 aggatcgtgg tgccggtctg ctcccagatc cggaggacct cgtcattcat ccgctcgcga
     2341 gtcatctcgt cgagcgcccc gaacggctcg tccatcaaga ggatcgacgg ctcgaaggcg
     2401 agcgcccggg cgatcgcgac gcgctgctgc atcccgcccg acagctggta cggatgatgc
     2461 ttcaggaagt cgccgagctc gacgagctcg agcatctcgc gggctcgttt cgtccgggtc
     2521 gcggcgtcct gaccgaggat ctcgagcggc agcttgacgt tgtcctcgac ggtcctccag
     2581 tcgaacagga ccggcgcctg gaagaccatc ccgtagtcgc ggtcgcgccg cgcccgctcg
     2641 gcgggcttgc cgttgaccgt gaccgtgccg ctcgacggcg aggtcaggtc gccgacgatc
     2701 cggaggagcg tcgacttgcc gcagcccgac ggtccgatca gggagacgaa ctcgccctgg
     2761 gcgatctcga ggtcgatccc ttcgagcgcc ttcgtcacga cctggtcacc gcgacggaac
     2821 gtcttgtcga cgccgctgat ccggacgacg ggcccgggcg ggttctgggt ggtcatggac
     2881 ggcgtgctcc tgtcaaggtg gtcaacccgc ggcggagaga accgggttgt tcggcccggg
     2941 cagggcgccg ggcgcgcgcc agggcgtgcc gccgaggaat ttcagcccct cccagacgac
     3001 cagcacggcg acgacgagca ggacccacgt cagggcccgg cggcgccgtc cggccaccgg
     3061 acgctccagc gcgacggcgg tccgtccacc tcgggtcgcc gtcgcggtcg ccatcgggct
     3121 agccggtcac cgcctggtcg gcgatgtcga gcgcccgatc gatgatcgcg aagccctcgc
     3181 ccagctgctc ctcggtgatg cacaggggcg ggttcgtgtg gatcgagtta ttggcgatca
     3241 tcgtgtagag gccgttctcg cggaagtact tcccgatcgc cttcatctcg tcgctcgtgc
     3301 cgttgaacgg tgtgagcggc gtccacggat cccggctccg gacgaggtcg aggatcccga
     3361 acaggccgag gttgcgatgg gccccgaccg aggggtggcg ggccgccagg tcctcgtgat
     3421 ggcggcgcat caccccgtcg aggcgggcgg cgttctcgat caggccgtcc tcctcgtaga
     3481 ccgcgatgtt ggccagcgcc gccgcgcacg agatcgcgtg gctcgagtag gtcaggccgc
     3541 cgtaatagaa gttcgtgtcg aagtgctcgg cgatcctgtg gttcatcgcg accgccccga
     3601 gcggcaggta cgacgaggtc aggcccttgg ccatcgtcag caggtccggg acggtgtccc
     3661 agtgctggat cgcgaaccac ttgcccgttc gcccgaagcc ggccatcacc tcgtcggcga
     3721 ccatcaggat gccgtgccgg tcgcacagct cgcggacgcc ctggatgtag ccgtcgggcg
     3781 ggatgaggat gccgttggtg ccgacgatcg tctcgaggaa gatcgccgcg atcgtgtggc
     3841 cgccctcgta gcggatgacc tcctcgaggc cctggagcgc ctcggcggcc gggcgcggct
     3901 cggcctcgcc ccagcgatgg gtgtccgggt agcggacgac gccgacgagg gccggctcgt
     3961 tggcccagcg gcgcgggtcg ccggtcaggg tcatcgcccc gagcgttgcg ccgtggtagg
     4021 cccggtagcg ggcgaggatc ttgtggcggc cggtgaaggc ccgggcgagc ttgatcgcgt
     4081 tctcgatcgc ctcggcccca ccgagggtga agaagacctt gtcgaggtcg cccggcagga
     4141 tctcggcgag cttcctgccg aggcgggctc gcggctcggt tgcgaaggcc ggctggacgt
     4201 actggagctt cgtcgcctga gcggtgatcg catcgatcac gcggcggtcg ccgtggccga
     4261 tgttgaccga catcagctgg ctgttgaagt cgaggatccg ctggccctcg ggggtgtaga
     4321 gatagacgcc ctctgcccgg tcgatcgcga tcggatcgat cgcggcctgg gcggaccacg
     4381 agaagaacgt atgagcgcgg gagagccgga cgatctcctc ggtggacatg ctcggctgag
     4441 cggtggtttc gacggccatg gcggctgacc tcggtgctgg tggcggtcgc cgccgaggca
     4501 cgacctcgtc aggcggcagc gggtgacgga ctgaccattc tcggggaccc gcgggagacc
     4561 attcggtcgg tccgccgggc tcgatccggc acacgattgt cccacaccgg caaccgcgcc
     4621 cgaccccgct tccggttgtg tcgccgcccc acgatgc
//
LOCUS       DATAIW010000323         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_109959, whole genome shotgun sequence.
ACCESSION   DATAIW010000323 DATAIW010000000
VERSION     DATAIW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109959"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VHM48_09920"
     CDS             complement(<1..375)
                     /locus_tag="VHM48_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2755771.1"
                     /translation="MPEVDVRLVDVVKKFGDAIAVDRINLEVRGGEFFSLLGPSGCGK
                     TTTLRMIGGFEQPTSGLIELQGQDVTWLPPFKRNVNTVFQNYALFPHLTIYENVAFGL
                     RRKGVKGGEIKSRVTEMLQLVEL"
     gene            1298..2377
                     /locus_tag="VHM48_09925"
     CDS             1298..2377
                     /locus_tag="VHM48_09925"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX2755772.1"
                     /translation="MPTLTEGAILEALSQVQEPELGRDIVTLQMVKDIVIEGTTAAFT
                     IELTTPACPLKDEIEGNTRAALTAIGVTDVTLTWGSMVRRSAPRQAEQLLPNVKNVIA
                     VGSGKGGVGKSTVSVNLAVALAQAGAAVGLLDADITGPNIPMMLGVDGQPKAAPNNKI
                     SPLERHGVKCISIQFFVPEGQPIVWRGPLVGGAIQQFLRDVDWGDLDYLVVDLPPGTS
                     DAQLTLAQSVPLGGAVLVTTPQEVALSDVGKALAMFKRLSVPILGLVENMTAFVCPHC
                     GELTEIFGRGGGERFCEEHQIDFLGGVPLDITVRQGGDVGVPAVAQREPGPAARALTA
                     VAGAVAARMSVRAASADPQPLLSIS"
     gene            2521..>4694
                     /locus_tag="VHM48_09930"
     CDS             2521..>4694
                     /locus_tag="VHM48_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2755773.1"
                     /translation="MRCRACGAVVGANARFCEQCGTRLHAPDSSAPYGGSTIGVDPPP
                     TARKTTRSFARTKAMASPPGSDRLAQDPSATGAAGDRRIVTALFADLVDYVRLVAEHD
                     PEEVRRRVEGALVAMVDAIQSFDGTREKFIGDAVFAVFGWPIAHDDDALRATHCALAI
                     RAGLARLEDPSGEVLQVRIGIATGEVVAAPREVPDAQDWSLTGPAVTTAARIQGVAQP
                     GEILLDEATIRAARRGLAIEDLGERLLRGQTRPIRIGRLLGEAGFQPWHPLAGNLVGR
                     NEELAIVRSALDDLHTNGRGATILVEGEAGIGKSRLLAALEQDARRLGIAWAWVDNVS
                     YGSSEPFRFARALSQAMADEHQTDSGTFLRRMLFSPDVDPADARRWAGGIAAVARAAS
                     FSGWEAEADLAPSDPADVARDVRAAAARYFERLVEADGPRIVVVDDLHWLDPSSVGVF
                     EELLAASARLPLLILVGSRADAARPGFLARGGVATITLTGLGEDETGELARAVAGATV
                     EAADVQRLHARTGGNPLFIGETVRAIVDEGAITSDGRLAIGDSADSVVPVTLRALLGS
                     RIDALSTDTRTVLRVGSVLGMSFREGTIEGVLGEPVDAAVYERLAEAAMIVPVDASGG
                     WRFCHPLIHDAAYRSLLATDRRTLHTRVADRIEAAQPDPPIGAIARHRAAAGDADRAI
                     PLLIRAAEQAQDLGAAAEAAAYFTSAADLAVGPTADALRERAS"
BASE COUNT          677 a         1683 c         1664 g          670 t
ORIGIN      
        1 cagctccacg agctggagca tctcggtgac gcggctcttg atctcgccgc ccttgacgcc
       61 cttgcggcgc agcccgaagg cgacgttctc gtagatcgtc aggtgcggga agagggcgta
      121 gttctggaag accgtgttga cgttgcgctt gaacgggggc agccaggtca cgtcctgacc
      181 ctgcaactcg atgagcccgg acgtgggctg ctcgaagccg ccgatcatcc gcagggtcgt
      241 cgtcttgccg cagcccgacg ggccgaggag gctgaagaat tcgccgccgc gcacctcgag
      301 gttgatccgg tcgaccgcga tggcgtcgcc gaacttcttg acgacatcga cgagccggac
      361 gtcgacctca ggcactcgcg cactcccgaa gcacggggcc acttctggcc ggccgaaaca
      421 cgaccggctt gggcgaccga gggttgccgt tccgcggacc tcctccgaac gcgggcggca
      481 ggccgaggat aggtgccgcc gcgagagggt gtcaacaatg cgaggtgcgc gcggtcaccg
      541 ggttgggcgg ttcgaatatc gttccgtgcg accagcgatc ccacaccgaa tcgcacgcga
      601 gcccagtgtc gcgtcggcgc ggccgcccgg ctggtggaga tgactggacg agacgtccag
      661 gaagaaccac gtcgacctgc ctgacatcgc gagcggcccg gaggcgtcgg ttcggctcac
      721 cagaagcgcg ggttcgctgc ccgggagccg atttctgcgg actcgcggga tggggcgtcg
      781 aagcgtccga gctctcggtg gcgcccgccc ggtcgcctca ccgtggtctc caggtcgccc
      841 cgtccagccg tgccggtcgt tggtcccgtt catgacaggt tcggttcatc agatgaacgg
      901 aatcgaccgc gccgttcatt ggatgaaccc atggagcatg gcgggccgtt cggcccatcc
      961 ggaatgactc aggacgcccc tgttcgggtc gagatggatc atcagatgag cgaaatcgac
     1021 ccgaatcggg tgacgatcgc gccccggatg tcgcataggt tcatcggatg aacgtcggcc
     1081 gggccgtgca cggaacggct ggcggcgaca ctcgcccacc gggacatcga ctccgccgcg
     1141 ccgcgccgcg ccgcgctgag ccaggcccgc catggccgaa gcgtggcggc tcgttccggc
     1201 ggtccgcggt aggatgccga tatcgacgca tcggctgaca gaagggccgc acccgtccgg
     1261 cgccgctcag ccacacgatc cagaggttcc ccaagcgatg ccgacgctca ccgaaggcgc
     1321 gatcctcgaa gcgctcagcc aggtccagga gcccgagctc gggcgggaca tcgtcaccct
     1381 ccagatggtc aaggacatcg tgatcgaggg cacgaccgcc gccttcacga tcgagctgac
     1441 gacgccggcc tgcccgctca aggacgagat cgagggcaac acccgggcgg ccctgacggc
     1501 gatcggcgtc accgacgtca cgctcacgtg ggggtcgatg gtccggcgct cggcgccgcg
     1561 ccaggcggag cagctcctgc ccaacgtcaa gaacgtcatc gccgtcggat ccggcaaggg
     1621 cggcgtcggc aagagcaccg tcagcgtcaa cctcgcggtc gcgctcgccc aggccggcgc
     1681 ggcggtcggg ctgctcgatg ccgacatcac cggcccgaac atcccgatga tgctcggcgt
     1741 cgacggccag ccgaaggcag ccccgaacaa caagatcagc ccgctcgagc ggcacggcgt
     1801 caagtgcatc tcgatccagt tcttcgtccc cgagggccag ccgatcgtct ggcgcggccc
     1861 gctcgtcggt ggcgcgatcc agcaattcct gcgtgacgtc gactggggcg atctcgacta
     1921 tctggtggtc gacctgccgc cgggcacgtc cgacgcccag ctgacgctcg cccagtcggt
     1981 cccgctcggc ggcgcggtcc tcgtcacgac gccccaggaa gtcgccctca gcgacgtcgg
     2041 caaggccctc gcgatgttca agcgcctgtc ggttccgatc ctcggcctcg tggagaacat
     2101 gaccgcgttc gtctgcccgc attgcggaga gctcacggag atcttcggtc gtggcggtgg
     2161 cgagcggttc tgcgaggagc accagatcga cttcctcggt ggcgtcccgc tcgacatcac
     2221 cgttcgccag ggcggcgacg tcggcgttcc ggccgttgcc cagcgcgagc ccggcccggc
     2281 ggcccgggcc ctgaccgcgg tcgccggtgc ggtcgcggcc cggatgagcg tccgggcggc
     2341 cagcgcggat ccgcagccgc tcctgtcgat cagctgagcg ccgggcgcta gactcgcacg
     2401 gcgggcggag gatcgctcgc gcctgacgaa ccggacgatc cgccggaacg ggacggacgc
     2461 cccaacggcc gccaaggccg gctcgagcac ggatcagagc acattgagga ggcggccccg
     2521 gtgcgctgtc gggcgtgtgg cgcggtggtc ggggcgaacg cgcgcttctg cgagcagtgc
     2581 gggacccgtc tccacgcccc cgacagctcg gcaccatacg gtggctcgac gatcggtgtc
     2641 gacccgccgc ccaccgctcg aaagaccacc cggagctttg cccggaccaa ggcgatggcg
     2701 tcgccgcccg gctccgaccg cctggcccag gatccgagcg caaccggcgc ggccggcgac
     2761 cgacggatcg tcacggccct gttcgccgat ctcgtcgact atgtccggct cgttgcggag
     2821 cacgacccgg aagaggtccg gcggcgggtc gagggagcgc tcgtcgcgat ggtcgacgcc
     2881 atccagagct tcgacgggac gcgcgagaag ttcatcggcg acgccgtttt cgcggtcttc
     2941 ggctggccga tcgcgcatga cgacgacgca ctccgggcga cccattgcgc tctggcgatc
     3001 cgggccggcc tcgcccgtct cgaggacccg tccggcgagg tgctccaggt ccggatcggg
     3061 atcgcgaccg gggaggttgt ggcggctccg cgtgaggttc ccgacgccca ggactggtcc
     3121 ctgacgggcc ctgccgtcac aaccgcggcg cgcatccagg gcgtggccca accaggcgag
     3181 atcctgctcg acgaggcgac gatccgggcc gcccggaggg gcctcgcgat cgaggatctc
     3241 ggcgagcgcc tgctccgcgg ccagacgaga ccgatccgga tcgggcggct gctcggcgag
     3301 gcgggcttcc agccgtggca tccgctggcg ggcaacctcg tcggcaggaa cgaggagctg
     3361 gcgatcgtcc ggagcgccct cgacgacctg cacaccaacg ggcgaggcgc gacgatcctc
     3421 gtcgagggcg aggccgggat cggcaagtcc cgcctcctgg ctgccctcga gcaggacgcc
     3481 cgacgcttgg gtatcgcctg ggcctgggtc gacaacgtgt cgtacgggtc gtcagagccg
     3541 ttccggttcg cacgggcgct gtcccaggcg atggccgacg agcaccagac cgactcgggg
     3601 accttcctgc ggcggatgct gttctcgccc gacgtcgacc cggccgatgc ccgccgctgg
     3661 gccggcggga tcgcggcggt cgcccgagca gcttccttct cgggctggga ggccgaggcc
     3721 gacctcgctc cctccgatcc ggccgatgtc gcccgcgacg tccgagccgc ggccgcccgg
     3781 tacttcgagc ggctcgtgga ggccgacggc ccgcggatcg tcgtcgtcga cgacctccac
     3841 tggctcgatc cgtcgagcgt cggcgtgttc gaggagctgc tcgccgcgtc ggctcgcctg
     3901 ccgttgctca tcctcgtcgg cagccgggcc gacgcggcgc gtcccggctt cctcgcccgc
     3961 ggcggcgtcg cgacgatcac cctcaccggg ctcggcgagg acgagaccgg cgagctcgcg
     4021 agggcggtcg ccggggcgac cgtcgaggcc gcggatgtcc agcgcctgca tgcccgcacg
     4081 ggaggcaatc cgctgttcat cggcgagacc gtccgggcca tcgtggacga gggcgcgatc
     4141 acgagcgacg gacggctcgc gatcggcgat tcggcggatt cggtcgtccc ggtcaccctg
     4201 cgggcgttgc tcggcagccg gatcgacgcg ctgtcgacgg acacccggac ggtgcttcgc
     4261 gtcgggtcgg tcctcggcat gagcttccgc gaggggacga tcgagggagt cctcggcgag
     4321 cccgtcgatg ccgctgtcta cgagcgtctc gccgaggcgg cgatgatcgt cccggtcgac
     4381 gcgtccggag gctggcggtt ctgccatccg ctgatccacg acgcggccta tcgcagcctc
     4441 ctcgcgaccg accgacggac gctccacacc cgggtcgccg accggatcga ggccgcccag
     4501 cccgatccgc ccatcggcgc gatcgcccgt caccgggcgg ccgccggcga cgccgaccgg
     4561 gccatccccc tgctgatccg ggccgccgag caggcgcagg atctgggcgc cgcggccgag
     4621 gccgccgcct acttcacctc cgcggccgac ctggccgtcg gaccgacggc cgacgcgctg
     4681 cgggagcgag cgtc
//
LOCUS       DATAIW010000324         8827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_217401, whole genome shotgun sequence.
ACCESSION   DATAIW010000324 DATAIW010000000
VERSION     DATAIW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217401"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHM48_09935"
     CDS             <1..242
                     /locus_tag="VHM48_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755774.1"
                     /translation="WGLEVTGCGPIGGTELVVTARVGRAAPVEVGTCSEGSYSSSMMA
                     LPLPPDGTTVALLTSGGTTKSQVRISGFQWRGERP"
     gene            complement(278..2443)
                     /locus_tag="VHM48_09940"
     CDS             complement(278..2443)
                     /locus_tag="VHM48_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2755775.1"
                     /translation="MFPVFADVPAYPLVFVIFWGSAAIFALAIARHLRVFAAARPAVE
                     QPRPFGQIGRRLIGLIEYAFVQRKMFKDPRAGLMHAGIFWGFVLLTIGTANIVTGGII
                     QTVIAAPFDGLLWAAVSAMQNVVAVIVIGSILWAYERRLISRPTRLTYNRDALLILAL
                     IGGVVATELLAQVFQFARYGDDPGAFVAAALAGPLRSAVGPGLLEAGFVVLWWAHIVI
                     VAVFLVDLPFSKHLHIATAFPNIWYRKLAPRGELPAMNLEREDATFGLKSLQDLGWKD
                     LLDGFTCTECGRCQAACPANFTGKPLNPKALIMGIRHMAVEAEHGLDLIPNNPLVRET
                     YGLGGTAPDAARMAAPIVDDAIPYDAVWDCVTCGACVEACPVLIEHVDKIVGLRRNLV
                     LEESRFPQELTGAFRGMEGQGNPWGQPASTRTDWTRGLPFEVPTVAAMAAAPGGLDDL
                     EVLYWVGCAAAFDERNKRVARSVATCLHAAGVQFAILGQEESCTGDPARRMGNDYVFQ
                     ILASGNVETLNRYGMGQRTIVTACPHCFNTISNEYGQLGGSFRVVHHSVYLQGLLASG
                     RLRVAEDGAGAAGSVTFHDSCYMARYNGVIAAPRDVLGAVPGLELREMEKSGRNSFCC
                     GAGGGRMWMEETRGTRINAERTRQALETGAATVATSCPFCMVMMKDGLADAAASSPAG
                     AGSPAGAAGVVSARDIAEILADVIEPDPIPAGRRLPVLQ"
     gene            complement(2443..3063)
                     /locus_tag="VHM48_09945"
     CDS             complement(2443..3063)
                     /locus_tag="VHM48_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX2755776.1"
                     /translation="MSDGPPPARTVLVLTTSDRSAAGTRDDTSGAVLADRLTALGFVV
                     ERAVVADDREEIEQALRDASDIHPLIVTTGGTGLTPRDVTPQATLAVIDYEVPGLAEA
                     IRAEGRTKTPFAALSRAVVGVRGHTLIVNTPGSPKGAAESLEAIVPVLDHALETLAGP
                     HDHAARSAGAVATSLDPDGDAPADSTEDQHAFGRREAPSSEPEEAD"
     gene            complement(3060..3662)
                     /gene="moaC"
                     /locus_tag="VHM48_09950"
     CDS             complement(3060..3662)
                     /gene="moaC"
                     /locus_tag="VHM48_09950"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX2755777.1"
                     /translation="MAREIPPRTERRRLTHVDRSGRPRMVDVSDKPSTARRAIAEALV
                     AVSAETMSMVIDGGGAKGDVLGVAELAGVMGGKRTSELIPLCHPLALTDLVVAVTPDR
                     AAGVLRVRAEAATTGPTGVEMEALTAASVAALTIYDMVKGVERGVEIRAVRLISKTGG
                     KSGEWHRPADPDAAREPDQRRPGDRAAGRIGGGNRRGPKA"
     gene            complement(3743..5098)
                     /gene="glp"
                     /locus_tag="VHM48_09955"
     CDS             complement(3743..5098)
                     /gene="glp"
                     /locus_tag="VHM48_09955"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX2755778.1"
                     /translation="MTVGAIGDRLLSVEEARAAVLAAIAGPTEPEMAYLAEALGRILA
                     DDVMSLTALPPWDNSAMDGYAIRAADVATATDIDPIRLEVVGEVRAGTAPETAVERGT
                     AIRIATGAPIPPRADAVVQVELTTPADAAGAPTGQRGRDAAGPLPAAVLVHEAIRAGT
                     AIRKVGDDLPEAMVILGAGTRLGPAAIALAAGSGNETVHVHRRPRVGILATGDEVRAA
                     GRALGPAGIPDANGPGLSALVEEAGGHSLALGIAADRLEDVRARLEAALHGDLDAIVV
                     SGGVSVGPYDVVRAAFAEIGRMELWRVAVQPGKPFAFGVVDRPAGGRTLLFGLPGNPV
                     SSFVTFELFVRPAIRRLAGLPAARLVRSSDRAVLVDEASKSLGRRAFLRVVAERDDAG
                     APIRDERGRVRVRLAGGGRGQGSHVLSALAIAEALAIVPETVDSVPVGGEVELWWLDR
                     D"
     gene            complement(5095..6222)
                     /locus_tag="VHM48_09960"
     CDS             complement(5095..6222)
                     /locus_tag="VHM48_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2755779.1"
                     /translation="MNGVPIARILGFEIRLHLSWLFIVAIITVTVASRLAQFQPEMDP
                     ALTWAIGLLGSLGFLATVIAHELAHAVVARRDGAVGRVLVVHFVGSAPIDVIASSPRT
                     EATTAIAGPLTSLVLGLILIALAVGSLSLGSWAGPVADVLIIIGMLDLLLAGISVVPA
                     FPLDGARLVRAAAWARTGNQRAGTKAAGVVGKYAGRATQIFALAIILAGETPTGIMVG
                     LVGWFLVASARSVDRWLVLDDLIQGMSVGEAMEQDLSTISPQLTLDTFAASILDGTVT
                     PALPVLHDDVLVGIIGLAQVRAVAQQKWPSTRTQDVMIGKPDMPTASPDDRLTDALER
                     LRQSHLDGLPVLDGETLRGVLTRRSIAALVHARAEARGQAR"
     gene            complement(6219..6962)
                     /locus_tag="VHM48_09965"
     CDS             complement(6219..6962)
                     /locus_tag="VHM48_09965"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEX2755780.1"
                     /translation="MTADSFSARSVPERLAAAATAVAASIVVIAIAILPFLTPAWVSF
                     EQGRTHAASLAGYTQADLAVATNAILHDLVLGPPDFAVEIAGAPVLEQREREHMRDVR
                     GVFAGFFLLAAIAAAGLVVLVAGARRMGHTERAWSAIASGMRWLILAIVVAGVIASVA
                     FDAVFEIFHRLFFPAGSFTFDPRTDHLVQLFPFDFWSETTIVLGVVIVAVAALISVGA
                     GRLASRTRAVAIGSPAVDPVGAGRAGVAR"
     gene            complement(7255..7641)
                     /locus_tag="VHM48_09970"
     CDS             complement(7255..7641)
                     /locus_tag="VHM48_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEX2755781.1"
                     /translation="MPPIGELTRIEQVHLDRLEHQGIFTTGLLLEVSETPTRRQTLAD
                     QVDASTNEVLTWRDEALMLNLASFGPEDHQLLMQAGIDGLHDILGLDLDAFRDRIRRG
                     ATELNMDPPDDLTTSGWWDQARTLED"
     gene            7763..8218
                     /locus_tag="VHM48_09975"
     CDS             7763..8218
                     /locus_tag="VHM48_09975"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2755782.1"
                     /translation="MSLFVLLKIVHILSAITALGANLTYPYWLRRAGRDRDRVLDAID
                     GISRLDRRIANPAYVLAFVSGAGMILTGFYSFGTLWVASAIALFVLVTILGITVYGPA
                     LRRQRAEAERDIASSAYAAIERRQNLLGLFVTALVVGIVVLMVSKPTLG"
     gene            8373..8822
                     /locus_tag="VHM48_09980"
     CDS             8373..8822
                     /locus_tag="VHM48_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2755783.1"
                     /translation="MSLDDNKALVRRFIDEIFVQGLPETVDELLAADFVAHTWPSTGN
                     PRADLKGAIERTSKGLADPRFTIEDMIAEGDQVAVRLTAEATHAGEFMGMPPSGKRYS
                     IGEIHVFRIRDGMVTEHWHQFDQMGMMKQLGAMPGGAPADGPAADSR"
BASE COUNT         1320 a         3165 c         3154 g         1188 t
ORIGIN      
        1 catgggggct cgaggtgacc ggctgtggtc cgatcggagg aacggagctc gtcgtcacgg
       61 cccgggtcgg cagggccgcc cccgtcgagg tcgggacgtg ctccgaaggc agctactcgt
      121 cgagcatgat ggccctgccg ctcccacccg atgggaccac ggtcgctctc ctcacgagtg
      181 gcggcacgac gaagtcgcag gtccggatct ccggattcca atggcgcggt gagcgcccct
      241 gatcgctcac cggcgaggac cgcgaagccc tcgcgcccta ctgcaggacc ggcagccggc
      301 ggccggccgg gatcgggtct ggctcgatca cgtcggcgag gatctcggcg atgtcgcggg
      361 cactgacgac gccggcggcg ccggccgggg aaccggcccc agccggcgac gaggcggcgg
      421 cgtcggcgag gccgtccttc atcatcacca tgcagaacgg gcagctcgtc gcgaccgtgg
      481 cagcacccgt ctcgagcgcc tggcgcgtcc gctcggcgtt gatccgcgtc ccgcgcgtct
      541 cctccatcca catccggccg ccgcctgcac cgcaacagaa cgagttgcga ccgctcttct
      601 ccatctcgcg gagctcgagg ccgggcaccg cgcccagcac gtcccgtggc gcggcgatga
      661 ccccgttgta gcgagccatg tagcaggagt cgtggaaggt caccgaacct gccgcgcccg
      721 cgccgtcctc ggcgacgcgc agccgcccgg acgccaggag cccctgcagg tagaccgagt
      781 ggtggaccac ccggaacgac ccgcccagct ggccatactc gttcgagatc gtgttgaagc
      841 agtgcggaca ggccgtgacg atcgtccgct ggcccatccc gtagcggttc agcgtctcga
      901 cgttcccgct ggcgaggatc tggaagacgt agtcgttgcc catccggcgg gccggatcgc
      961 cggtgcacga ctcctcctgg ccaaggatcg cgaactgcac gccggcggca tggaggcatg
     1021 tcgcgaccga ccgcgccacc cgcttgttgc gctcgtcgaa cgccgcggcg cagcccaccc
     1081 agtacaggac ctcgaggtcg tcgaggccgc ccggtgccgc cgccatcgca gccacggtgg
     1141 ggacctcgaa cggcaggccg cgggtccagt ccgttcgcgt cgaggccggc tggccccacg
     1201 ggttgccctg gccctccatg ccgcggaacg cgcccgtcaa ttcctgcggg aagcgcgact
     1261 cctcgaggac gaggttccgc cgcagcccga cgatcttgtc gacgtgctcg atcagcacgg
     1321 ggcaggcctc gacgcacgcg ccgcacgtga cgcagtccca gaccgcgtca taggggatgg
     1381 cgtcgtcgac gatcggcgcc gccatccggg ccgcgtccgg cgctgtgccg cccagcccgt
     1441 acgtctcgcg gacgagcggg ttgttcggga tcaggtcgag cccgtgctcc gcctcgacgg
     1501 ccatgtgacg gatccccatg atgagggcct tgggattgag cggcttgccc gtgaagttgg
     1561 cgggacaggc ggcctggcag cggccgcact cggtgcaggt gaagccgtcg aggaggtcct
     1621 tccagccgag gtcctggagg gacttcaggc cgaacgtggc gtcctcccgc tccaggttca
     1681 tggccggcaa ctcaccgcgg ggcgcgagct tgcgatacca gatgttcggg aacgcggtcg
     1741 cgatatggag atgcttgctg aacggcaagt ccacgaggaa taccgccacg atcacgatgt
     1801 gcgcccacca caggacgacg aagccggcct cgagcaggcc cggcccgacc gccgaccgga
     1861 gcggcccggc gagcgcggcg gcgacgaacg ctcccggatc gtcgccgtag cgcgcgaact
     1921 ggaagacctg ggcgaggagc tcggtcgcga caaccccacc gatcaacgcg aggatcagga
     1981 gcgcgtcgcg gttgtaggtg aggcgcgtcg gccgtgagat caggcgccgc tcgtaggccc
     2041 acaggatcga gccgatcacg atcaccgcga cgacgttctg catcgcgctg accgcggccc
     2101 acagcaggcc gtcgaacggg gccgcgatga cggtctggat gatgccgccc gtgacgatgt
     2161 tcgcggtccc gatcgtcagc aggacgaagc cccagaagat gcccgcgtgc atcaggccgg
     2221 ctcgcgggtc cttgaacatc ttcctctgga cgaaggcgta ttcgatcagc ccgatcaacc
     2281 gtcgcccgat ctggccgaac gggcgcggct gctcgacggc gggtcgcgcc gccgcgaaga
     2341 cgcgcaggtg gcgggcgatc gcgagggcga agatcgcggc cgacccccag aagatgacga
     2401 agaccagcgg gtaggcgggc acgtccgcga agaccgggaa catcagtcgg cttcctccgg
     2461 ttcactggac ggggcctcgc ggcggccgaa ggcatgctga tcctcggtgc tgtcggccgg
     2521 cgcgtcgccg tccgggtcga gcgaggtcgc cacggcaccg gccgaccgcg ccgcgtgatc
     2581 gtgcggcccg gccaacgtct cgagggcgtg gtcgaggacc gggacgatcg cctcgagcga
     2641 ctccgccgca cccttcggcg agcccggcgt gttcacgatc agggtgtgcc cgcggacccc
     2701 gacgaccgcc cggctcagcg cggcgaacgg ggtctttgtc cggccctcgg cgcggatcgc
     2761 ctcggccagg ccggggacct cgtagtcgat gacggcgagc gtcgcctggg gcgtcacgtc
     2821 gcgcggggtg aggcccgtcc cgccggtcgt cacgatgagc ggatggatgt ccgaggcatc
     2881 gcgcagcgcc tgctcgatct cctcccgatc gtcggcgacg accgcccgtt cgaccacgaa
     2941 gccaagggcc gtcaaccgat ccgccagcac tgccccgctc gtgtcgtcac gggtccccgc
     3001 cgcgctccgg tcgctcgtgg tgaggaccag cacggtcctg gcgggcggcg ggccatcgct
     3061 catgccttcg ggcctcgtcg attgcctccg ccgatccggc ccgcggcccg gtcacccggg
     3121 cgccgctggt ccggctcgcg cgccgcgtcg gggtcggccg ggcggtgcca ctcgccgctc
     3181 ttgccaccgg tcttcgagat caggcgaacg gcacggatct cgaccccgcg ctcgacgccc
     3241 ttgaccatgt cgtagatcgt cagggccgcg acggacgcgg cggtcagggc ctccatctcg
     3301 accccggtcg gcccggtcgt cgctgcctcc gcccggaccc gcaggacgcc ggcggcccgg
     3361 tccggcgtca ccgcgacgac gaggtcggtc agggcgagcg gatggcagag cgggatcagc
     3421 tcgctcgtcc gcttgccgcc catcaccccg gccagctcgg ccacgccgag gacatcgccc
     3481 ttcgctccgc ccccgtcgat gaccatgctc atcgtctcgg ccgacaccgc gacgagcgcc
     3541 tcggcgatgg cccgccgcgc agtggacggc ttgtcggaca cgtcgaccat cctgggccgg
     3601 ccggatcggt cgacatgggt caggcgacgg cgctcggtgc ggggcgggat ctcgcgggcc
     3661 atggctgcct ccagtatcgg tcgatcggac gggccgcgag cggcctgcgg acgcggggcg
     3721 cgggtgacgg gagtggatcc ggtcagtcgc ggtcgagcca ccacagctcg acctcgccgc
     3781 cgactggcac ggaatcgacg gtctcgggca cgattgcgag cgcctcggca atcgcgagcg
     3841 ccgacaggac gtggcttccc tgcccgcggc cgccgcccgc gagccggaca cggacgcgac
     3901 cgcgctcgtc acggatcggc gcaccggcgt cgtcccgctc cgcgaccacc cgcaggaagg
     3961 cgcgtcggcc caggctcttc gacgcctcgt cgaccagcac cgcccggtcc gacgaccgga
     4021 cgagccgcgc cgccggcaac ccggcgaggc gacggatggc gggacggacg aacagctcga
     4081 acgtcacgaa gctcgacacg gggttcccgg gcagcccgaa cagcaaggtc cgcccgccgg
     4141 cgggccggtc gacgacgccg aatgcgaacg gcttgccggg ctggacggcc acccgccaca
     4201 gctccatccg cccgatctcg gcgaaggccg cgcggacgac gtcgtacggc ccgaccgaca
     4261 cgcccccgga caccacgatc gcgtcgaggt ctccgtgcag cgcggcctcg agacgggccc
     4321 ggacgtcctc gagccggtcg gcggcgatcc cgagcgccag cgagtggccg cccgcctcct
     4381 ccaccagcgc actgagcccg ggcccgttcg catccgggat cccggcgggg ccgagcgccc
     4441 tgcccgcggc gcggacttcg tcgccggtgg cgaggatccc gaccctgggc ctgcgatgga
     4501 cgtggaccgt ttcgttgccg gagcccgcgg ccagcgcgat cgcggccggc ccgaggcggg
     4561 tccccgcgcc caggatcacc atcgcctccg gcaggtcgtc gcccaccttc cggatcgcgg
     4621 tcccggcccg gatcgcctcg tggacgagga cggcggccgg gagtggaccg gcggcgtcgc
     4681 ggccccgttg cccggtcggc gcgccggccg catccgcggg cgtggtcagc tcgacctgga
     4741 cgacggcgtc ggcacggggc gggatcgggg cccccgtcgc gatccggatc gccgttcccc
     4801 gctcgaccgc ggtctctggc gcagtgccgg cccggacctc gccgacgacc tcgaggcgga
     4861 tcgggtcgat gtccgtggcc gtcgcgacat ccgcggcccg gatcgcatac ccgtccatcg
     4921 cggagttgtc ccacggcggc agggctgtca ggctcatcac atcgtcggcc aggatccggc
     4981 cgagcgcctc cgccagatag gccatctcgg gctcggtcgg gccggcgatc gccgccagga
     5041 cggctgcgcg cgcctcctcg accgagagaa gtcgatcgcc gatcgccccc accgtcaccg
     5101 ggcctgcccg cgcgcctcgg cccgggcgtg gaccagcgcg gcgatggagc gccgcgtcag
     5161 gacaccgcgc agcgtctcgc cgtcgagcac cggaagtcca tcgagatgcg actggcgcag
     5221 tcgctcgagc gcgtcggtca ggcggtcgtc cgggcttgcc gtcggcatgt cgggcttgcc
     5281 gatcatcacg tcctgggtgc gagtggacgg ccacttctgt tgggcgacgg ctcggacctg
     5341 ggccaggccg atgatcccga ccagcacgtc atcgtgcagc accggcagcg ccggcgtgac
     5401 cgttccgtcg aggatgctgg ctgcgaaggt gtcgagcgtc agctgtggcg agatcgtcga
     5461 gagatcctgc tccatcgcct cgccgacgct catcccctgg atcaggtcgt cgagcaccaa
     5521 ccagcgatcg accgaccggg ccgacgccac gaggaaccag ccgaccaggc caaccatgat
     5581 cccggtgggc gtctcaccgg ccaggatgat ggcgagggcg aagatctggg ttgctcgccc
     5641 ggcgtatttc ccgacgacac cggcggcctt cgtccccgca cgctggttgc cagttcgagc
     5701 ccacgccgcc gcccggacga gccgggcgcc gtcgagcggg aatgccggaa cgacgctgat
     5761 gccggccagg agcaggtcga gcatgccgat gatgatgagg acgtcggcca cgggcccggc
     5821 ccacgatccg aggctcagcg aacccaccgc gagcgcgatg aggatcaacc cgaggacgag
     5881 gctcgtgagt gggccggcga tcgcggtggt ggcctccgtg cggggcgagc tggcgatcac
     5941 gtcgatcggc gccgagccga cgaaatggac gacgagcacg cggccgaccg cgccatcgcg
     6001 gcgggcgaca acggcatgcg cgagctcgtg ggcgatgacg gtcgcgagaa acccgagcga
     6061 gccgaggagc ccgatcgccc aggtcagggc cggatccatc tccggctgga actgggccag
     6121 acgactggcg accgtgaccg tgatgatcgc gacgatgaag agccacgaca ggtggaggcg
     6181 gatctcgaag ccgaggatcc gggcgatcgg gacgccgttc atcgggcgac cccggctcgg
     6241 ccggcgccga ccgggtccac cgccggcgac ccgatggcaa cggctcgggt tcgactcgcg
     6301 aggcgcccgg caccgaccga gatgagcgcg gcgacggcca cgatgacgac gccgaggacg
     6361 atcgtcgtct ccgaccagaa gtcgaacggg aagagctgga cgagatggtc cgtccgggga
     6421 tcgaacgtga aggaccccgc gggaaagaac aaccggtgga agatctcgaa tacggcgtcg
     6481 aacgcgaccg aggcgatcac cccggcgacg acgatcgcga ggatcagcca gcgcatcccg
     6541 gacgcgatcg ccgaccacgc ccgctcggtg tggcccatcc gccgcgcacc ggcgacgagg
     6601 acgacgaggc ccgccgccgc gatcgcggcc agcaggaaga acccggcgaa caccccgcgg
     6661 acatcacgca tgtgctcacg ctcgcgttgc tcgaggacgg gtgcgccggc gatctccacc
     6721 gcgaagtcgg gtggcccgag cacgagatcg tggaggatgg cgttggtcgc gacagcgagg
     6781 tcggcctgcg tgtagcccgc caggctggcg gcgtgcgtcc gaccctgctc gaacgacacc
     6841 caggcggggg tcaggaaggg caggatcgcg atggcgatca cgacgatcga cgcggcgacg
     6901 gccgtcgccg ccgcggccag gcgctccggc acggaccgtg cgctgaagga gtctgcggtc
     6961 accgccgcat ggtagaccgg gcggacggcc tgcgccgccg aaccgccgaa gccgatgggg
     7021 cgctggttcg tcattcacca tcggccgtgg tgcgtgtcgg ccgcggtcac ccggattcga
     7081 ccatgcctgc ccggacgatc ccgcctgaca ccatcccccc gatcgtggtt gacgcaggaa
     7141 tcgaccatcg gcgaccgagt cgaaggccgt ggggacgtca cgatccggac ttcaaccacc
     7201 gggggtggtg agtgacacga acgacgccgc cgcgtggcgg gcccgcgggt ccggtcagtc
     7261 ctcgagggtc cgcgcctggt cccaccagcc cgaggtcgtg agatcgtcgg gcgggtccat
     7321 gttcagctcg gtggcgcccc gccggatcct gtcgcggaac gcatcgaggt ccagcccgag
     7381 gatgtcgtgc aggccgtcga tcccggcctg catcaggagc tggtggtcct ccggcccgaa
     7441 gctcgcgagg ttgagcatca gggcctcgtc gcgccaggtc aggacctcgt tggtcgacgc
     7501 atcgacctgg tcggcgagcg tctggcgtcg ggtcggcgtc tcgctgacct cgagcagcag
     7561 gccggtggtg aagatgccct ggtgctcgag ccggtcgagg tggacctgct cgatccgggt
     7621 cagctctccg atcggtggca cggtgacagt tggacctcag cgacggaacg ggtgatcgac
     7681 tcgatcatgc cacggcggtc gcgtcgggtg cacgtcggcc gctaccatcg accccgattc
     7741 gtgccgaccc ggagggaccg ccgtgagtct gttcgtgctc ctgaagatcg tccacatcct
     7801 gtccgcgatc accgccctcg gggcgaacct cacgtatccg tactggctcc gccgagccgg
     7861 gcgagaccgc gaccgggtgc tcgacgcgat cgatgggatc tctcgactcg accgccggat
     7921 cgcgaacccg gcgtacgtcc tggcgttcgt cagcggagcc gggatgatcc tgaccggctt
     7981 ctactccttc ggcacgttgt gggtggcgag cgcgatcgcg ctgttcgtgc tcgtgacgat
     8041 cctcgggatc acggtctacg gcccggcgct ccgccgacaa cgcgcggagg cggagcgcga
     8101 catcgcttcg tcggcgtacg ccgcgatcga gcgccgccag aacctgctcg gcttgttcgt
     8161 gaccgcgctg gtcgtcggga tcgtcgtgct catggtctcc aagccgacgc tcggatgaac
     8221 ccgtccgtcg acggctcgtc ggcgatccgc ccgcgtcggc tcgtggcggg agacacggtg
     8281 tcgcggggcc gtcgcttcgg cccggcggac cgagaggcgg cgttgaggac gcggccccca
     8341 ggctgggcga cacgcaacaa ggaggaatac ccatgagcct cgacgacaac aaggcgctcg
     8401 tccgccggtt catcgacgag atcttcgtcc agggcctgcc cgagacggtc gacgagcttc
     8461 tcgcggccga cttcgtggcc cacacctggc cgtcaaccgg caacccgagg gccgacctca
     8521 agggggcgat cgagcgcacc tcgaagggcc tcgccgaccc caggttcacg atcgaggaca
     8581 tgatcgccga gggcgaccag gtcgccgttc gcctcaccgc cgaagcgacc cacgccggcg
     8641 agttcatggg gatgccgccg agcggcaagc gctattcgat cggcgagatc cacgtcttcc
     8701 ggattcgcga cggcatggtc accgagcact ggcaccagtt cgaccagatg gggatgatga
     8761 agcagctcgg ggcgatgccc ggtggcgcgc cggccgacgg tccggcggcg gactcgcgct
     8821 gaccccg
//
LOCUS       DATAIW010000325         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_187683, whole genome shotgun sequence.
ACCESSION   DATAIW010000325 DATAIW010000000
VERSION     DATAIW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187683"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..113)
                     /locus_tag="VHM48_09985"
                     /pseudo
     CDS             complement(1..113)
                     /locus_tag="VHM48_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(490..1431)
                     /locus_tag="VHM48_09990"
     CDS             complement(490..1431)
                     /locus_tag="VHM48_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755784.1"
                     /translation="MRSRRRIGLAAVAVLVPALLGASSITTLARLTDADTSSKAIATD
                     TLDPPTTLAATGGSSASLTWTATTDTYASGYEVRRATVSGGPYSLITTVTPRTTVATT
                     DAPGSNGTYYYVLRSAFQNWRSVNSNQASATITLGSTPTGFKTCTTTSNAADTGGDGD
                     GYESVPTKACVTDGSIATDANTGTNTSLVCLDAGKDRHRFWDFTLGVPATVSAVGGIQ
                     VRTDVGLNNNSGTSQICAQLSWDAGTTWTAARSVAVANVGVTTYTLGATNDTWGRSWL
                     GSDFSNATFRVRLIDTSDRTSKDFRLDGLAVQVTYTP"
     gene            complement(1435..2271)
                     /locus_tag="VHM48_09995"
     CDS             complement(1435..2271)
                     /locus_tag="VHM48_09995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2755785.1"
                     /translation="MRILAGATTVLRRIVDLGLVILIVVVLLGVILGKGAPLVGRQSI
                     VIGGGSMEPTIGLGAVIVVRPVAPASLAVDDVVSMQVGPDRATYTHRIVALVDRPDGR
                     WIRTKGDANAAPDPTLVPASAVIGRVEFVIPLAGYLLALLSLPMGIMFVLGLAATLLA
                     MAWLLESLEPEPRPVRRPAGADAIDPLPEPTSAGLPAAASVLDPELLDGEPIAARPVR
                     IADSSLGGATWAFAAPALAPAISGSLARPTVRAQIERSREVRQRRARWLIGRGHSRSA
                     AD"
     gene            complement(2446..3036)
                     /locus_tag="VHM48_10000"
     CDS             complement(2446..3036)
                     /locus_tag="VHM48_10000"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEX2755786.1"
                     /translation="MHSQLTDHQVERRRKRRRGIVALLGAVSILTIGAGSISLAQFTD
                     SDSSTWAFTTGTIDINSNPAVLTAVSGMMPGGSSTQALTISNDGSGDLRYAMSVAATN
                     ALGSALQLTVKAEDAGGGCAAFTGASVLATTTLNGAGWGSSAQGFQAGDRNLTAGTNE
                     ILCFRVSLPLATDDTYQGASSAATFTFAAEQTANNP"
     gene            complement(3394..4218)
                     /locus_tag="VHM48_10005"
     CDS             complement(3394..4218)
                     /locus_tag="VHM48_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2755787.1"
                     /translation="MTDEPVLLREPTPWGIRLTLNRPAKLNAISAELRDALTAAISEA
                     TADARVRVIVIAGAGRAFCSGYDLSESAPATAWDWRDVLARDVEATLAVWSCPKPVIA
                     QVHGYALAGGLELAMACDLIVAAEGAQLGEPEIRFGSAPVTLLMPFVIGQKRTRELLL
                     TGDLIGAVEAERIGLINRVVPVDRLVEEVDTLAEKLARVPPDVMAPTKLMLNRAMDAA
                     GFAAAVEMGLDLQSFVNMSETAREFDAIVRREGLKAALAWRDRRYEERLAARPTED"
     gene            complement(4215..>4930)
                     /locus_tag="VHM48_10010"
     CDS             complement(4215..>4930)
                     /locus_tag="VHM48_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2755788.1"
                     /translation="VGEPAVGEPALGEPAADLAAGPLALPEVESLVRLEAAGLPVLRQ
                     VRVADADGAVAAATELGFPVVLKIDVVGLVHKSDAGAVRLGLTNGAAVRSAAAELLAL
                     PLPAGAVRRGLLVARQLSGPELIVGGRRDPTFGPIVIVGLGGILADVLDDVAIRLAPL
                     DPGTAEAMLDDLRGAAILAGARGRAAIDRSAVAQAIVRLGDLLVADPTILEIDCNPLI
                     SGVDGTAAVDALVVLEGEP"
BASE COUNT          734 a         1670 c         1829 g          697 t
ORIGIN      
        1 atccaggacg ccgaggtcgg ggtgaagtgg tggtgaaagt gcttgtgacg ggcgagccac
       61 cgttggatct cgggcgtctt gcgggtcgac acgttgtcaa ggacgacgtg gatctggcgg
      121 cgcggatagg tccgggccac cgtagctgcg gccatcaagt acctacaagc ctttagtcga
      181 gcgggacact agaggatcat cagcggtcgc tgacgccgtg catcctgcga cgaaggtcgg
      241 gaggcccggg acgaggctgc ctgccatcag gtcggtcacg ttgtctttgg gcccaatgcg
      301 aacgacacgc agttcaaatc gccgtgcata cgtctggacg acgccccgcc gtcgccgtcg
      361 atcctcggct ctccgttcgg ccagggccct cctcgcgacg aggtgggcct gatcgagcac
      421 atggcgcgcc ctgcgcacgt gccctcatgc ggcggacatt gcgccgctct atcagcgagg
      481 caggcgtggc tacggcgtgt acgtcacctg cacggcgaga ccgtcgaggc ggaagtcctt
      541 cgaggtccga tccgaggtgt cgatcaggcg aacgcggaag gtggcgttcg agaagtcgga
      601 cccgagccac gaccggcccc atgtgtcgtt ggtggcaccg agggtgtagg tcgtaacccc
      661 gacattcgcg accgccacgg acctggccgc cgtccacgtg gtccccgcgt cccacgagag
      721 ctgagcgcag atctggctcg tgccgctgtt gttgttcagg ccgacatcgg tccggacctg
      781 gatgccgccc acggcgctga ccgtcgccgg gacgcccagg gtgaagtccc agaaccggtg
      841 gcggtccttg cccgcatcga ggcagacgag gctcgtgttc gtgccggtgt tggcgtcggt
      901 cgcgatcgac ccgtcggtca cgcacgcctt cgtcgggacg gattcataac catcgccgtc
      961 accgccggtg tccgcggcgt tcgaggtcgt ggtgcacgtc ttgaagccgg tcggggtcga
     1021 cccgagcgtg atcgtcgccg acgcctggtt gctgttgacg ctccgccagt tctggaacgc
     1081 gctgcgcagg acgtagtagt acgtcccgtt cgagccgggc gcgtcggtcg tggcgacggt
     1141 cgtccgcggc gtgaccgtgg tgatcaggct gtacgggccg cccgacacgg tcgcccgccg
     1201 gacctcgtag ccggacgcat acgtgtcggt cgtggcggtc caggtcaacg aggcgctcga
     1261 acccccggtc gcggcgagcg tcgtcggcgg atcgagcgtg tcggtcgcga tcgccttgct
     1321 gctcgtgtcc gcgtcggtca ggcgggccag ggtcgtgatc gacgacgctc cgaggagcgc
     1381 cgggacgagc accgcaacgg cggcaaggcc gatgcggcgg cgggatcgca tcagtcagtc
     1441 ggccgcgctg cggctgtgtc cgcgaccgat cagccagcgg gctcgccgct ggcggacctc
     1501 gcgactccgt tcgatctggg cgcggaccgt cggtcgtgcg agcgacccgg agatcgccgg
     1561 cgcgagagcc ggagcggcga acgcccacgt ggccccgccg aggctcgagt cggcgatgcg
     1621 gaccggtcgg gcggcgatcg gctcgccgtc gaggagctcg ggatcgagca ccgacgccgc
     1681 ggccggcagg cccgcggacg tcggctcagg cagaggatcg atggcgtcgg cgccggcggg
     1741 acggcgcacc gggcgaggct cgggctccag ggattcgagc agccaggcca tggccagcag
     1801 cgtcgccgcc aggccgagca cgaacatgat gcccattggc agcgacagca gcgccagcag
     1861 gtacccggcg aggggaatga cgaactcgac ccggccgatg accgcgctgg ccggcacgag
     1921 ggtcggatcg ggcgcggcgt tggcgtcgcc cttcgtccgg atccagcgtc cgtcggggcg
     1981 gtcgacgagc gcgacgatcc ggtgggtata ggtcgcccgg tccggtccga cctgcatcga
     2041 gacgacgtcg tcgaccgcga gggaagccgg ggcgacgggt cggacgacga tcaccgcgcc
     2101 gagcccgatc gtcggctcca tcgagccgcc gccgatgacg atcgactggc ggccgacgag
     2161 cggcgcaccc ttgccgagga tcacgccgag cagcacgacg acgatcagga tgacgaggcc
     2221 gaggtcgacg atccggcgaa gaaccgtggt ggcgccggcg aggatcctca tcgttgattc
     2281 ctccaggaac gcgatgggct gctgttcacc tgcgagccgg cccgacggtc cgtgacgcca
     2341 gggcgggcgc catgccggcc aacccgcagg tcggtccgga agcgaccggg ctgcggctcg
     2401 cccacgagga cgagccgcag ccggtggtgc agccggagac tgcggttacg ggttgttggc
     2461 ggtctgctcg gccgcgaagg tgaacgtggc ggcagagctc gcgccctggt aggtgtcgtc
     2521 ggtcgcgagc ggcaggctga cccggaagca gaggatctcg ttggtcccag cggtcaggtt
     2581 gcggtcgccc gcctggaagc cctgggcgga gctgccccag cccgcgccgt tgagcgtcgt
     2641 cgtcgcgagc acggaggcgc ccgtgaaggc ggcgcatccg ccgcccgcgt cctcggcctt
     2701 gaccgtcagc tggagggccg atccgagagc attggtggcc gcgaccgaca tcgcgtagcg
     2761 gaggtcgccg gagccgtcgt tcgagatcgt cagcgcctgg gtcgacgagc caccgggcat
     2821 catcccgctg accgcggtga ggacggccgg gttggagttg atgtcgatcg ttccggtcgt
     2881 gaacgcccag gtcgacgaat cgctgtcggt gaactgggcg agggaaatcg agcccgcgcc
     2941 gatcgtcagg atcgagacgg ccccgagcag ggcgacgatg ccgcggcggc gctttcggcg
     3001 acgctcgacc tggtgatccg tgagctggct gtgcatgaac gtgggtcctt tcccgaagcg
     3061 ttgatgaggc ctgaccgccg gcggaggccg gtcgtggatg cgcgttcccc ccactggacg
     3121 tgcggccggt tcgggcgagg ggtttcgcgg tgcccgaacg ggtgacgccg ttcaggggga
     3181 gtgaacgacc tgaacgacgt acgtcgctac gggactacgg gaccaatcga acccgtacct
     3241 tggtcccatc cagtcgagtg gggacgcggc cgaagagcag cggtgcgcgg gctgcgccct
     3301 gccggcgccc gtgggcacac cgttggggag tgaaccgggc cgatggtccg tcctcgaatc
     3361 agggcgcctg cggcggttcg gccgccgacc gaatcagtcc tcggtcgggc gggcggcgag
     3421 ccgctcctcg tagcgccgat cccgccacgc cagcgccgct ttcaggccct cgcggcggac
     3481 gatcgcgtcg aactcgcggg cggtctcgct catgttgacg aaggactgga gatcgagccc
     3541 catctcgacc gcggccgcga acccggccgc gtccatcgcc cggttgagca tcagcttcgt
     3601 cggggccatc acgtcgggcg ggacgcgcgc gagcttctcg gcgagtgtgt cgacctcctc
     3661 caccagacga tcgaccggca cgacccgatt gatcaggccg atccgctcgg cctcgacagc
     3721 accgatcagg tcgccggtca ggaggagctc gcgcgttcgc ttctggccga tcacgaacgg
     3781 catcagcagc gtgacaggcg ccgacccgaa ccggatctcc ggctcgccga gctgagcgcc
     3841 ctcggcggcg acgatcaggt cgcaggccat ggcgagctcg agcccgccgg cgagtgcata
     3901 gccatgcacc tgggcgatca ccggcttcgg gcaggaccag accgcgagcg tcgcctcgac
     3961 atcgcgggcg aggacgtcgc gccagtccca ggcagtcgcc ggcgctgatt cggacaggtc
     4021 gtagccggag cagaacgccc ggcccgcccc ggcgatgacg atcacccgga cacgcgcgtc
     4081 ggccgttgcc tcggagatcg ccgcggtcag cgcgtcgcgc agctcggcgc tgattgcgtt
     4141 gagcttggcc ggccggttca gggtcaggcg gataccccac ggcgtcggct cgcggaggag
     4201 gaccggctcg tcggtcacgg ctcgccctcc agcacgacga gggcatcgac ggccgccgta
     4261 ccgtccacgc ccgagatcag cgggttgcaa tcgatctcga ggatcgtcgg gtccgcgacg
     4321 aggaggtcac cgagccggac gatcgcctgc gccaccgcgg accggtcgat cgccgcccgg
     4381 ccgcgagcgc cggcgaggat cgccgcgccg cgcaggtcgt cgagcatcgc ctcggcggta
     4441 ccggggtcga gcggggccaa ccggatcgcg acatcgtcga ggacgtccgc caggatcccg
     4501 ccgagaccga cgatcacgat cggcccgaag gtcggatcgc gccggccacc gacgatcagc
     4561 tccggaccgc taagctgccg cgcgacgagc aggccacgcc ggacggcgcc cgcgggtagc
     4621 ggcagggcca gcagctctgc cgcggcggac cggacggcgg caccgttggt cagccccagg
     4681 cgaaccgccc cggcatcgct cttgtggacc agcccgacga cgtcgatctt gaggacgacc
     4741 gggaagccga gctcggtggc ggccgcaacc gcaccgtctg cgtcggcgac ccggacctgc
     4801 cgcagcaccg gcaggcctgc cgcctcgagc cgaacgaggc tctcgacctc gggcagggcc
     4861 agtggaccgg cggcgaggtc ggcggcaggc tctccgaggg cgggctctcc gacggcgggc
     4921 tctccgacgg
//
LOCUS       DATAIW010000326         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_50526, whole genome shotgun sequence.
ACCESSION   DATAIW010000326 DATAIW010000000
VERSION     DATAIW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50526"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..644)
                     /locus_tag="VHM48_10015"
     CDS             complement(366..644)
                     /locus_tag="VHM48_10015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755789.1"
                     /translation="MPVWSIVCFVVSRTVRGQGLTTRLLDAAMEYARAHGAPGLEAYP
                     VDAGDGRVPAALGYTGHLSTFLAAGFRVVHEIDSPQATVRRVIVRRDA"
     gene            complement(765..1841)
                     /locus_tag="VHM48_10020"
     CDS             complement(765..1841)
                     /locus_tag="VHM48_10020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2755790.1"
                     /translation="MRDSGWFAHGRRLFRLVDEDVRALLPAIRWVGTFLLVVWFAWQT
                     YDRVTFFTNQGFPVGIDATIYYRGVVAWLDGGDPWDAAVVVNGWSFHYAGSPVTTVLM
                     APAALVSEVTFTRIWVLATWASAVWTLRRLNLPLWWLLFPPIGEALFSANPQIVVLAL
                     LVANHPLASAVATGLKVYAFIPLVGEGRWRHAWAGVGLVGATAVIASDLWIDYIGKLG
                     FISRRLMDESTKGGSAFYYPELLAVTVLALVLLALRDRRTAGWLAVPAVWPASQFHYS
                     TMALPVMSPFLAVFLALPATHAAPIAIVLEAGRRLIQPLVMRLVASLAGPPETVDVGD
                     DAMPAPKMTGPDRPAEYHPRHDRE"
     gene            1970..2803
                     /locus_tag="VHM48_10025"
     CDS             1970..2803
                     /locus_tag="VHM48_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755791.1"
                     /translation="MIDGVLDAELAGLAWLLVEARLPLIVAGLHRGAGKTTLFEALLD
                     FLPSTARRIDLAGATEEFAWLPEAGALGWPAAGERAEPSSIPITPATGFLVAAELSEH
                     LPTHTWGRAARTLVRAASIGYGIGATVHADRLEDVHDLLRGSPVGLTDDELTYLGLIL
                     IVRATSEPDGSVRRRVVAAHYARPVSRDEHGHTQRPAPAVLASRDEGADHLEHFAWGV
                     MPELAIRLGRKAGDIERDQFERAALLTALAQDRTTGVDEVRAAIRDARRLPTPAMDHL
                     H"
     gene            2860..>3384
                     /locus_tag="VHM48_10030"
     CDS             2860..>3384
                     /locus_tag="VHM48_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755792.1"
                     /translation="MPSPSVDRQRRPETLGLLRAAGWSSRTVKQELRSNLLARLAADR
                     PIVSGIVGYDESVLPAIENAVVAGQDLVLLGERGQAKTRLARLLVGLLDDAIPIVRGG
                     ELNDDPYAPISPSGKAIVAEHGDATPIDWLPRDRRYSEKLATPDITIADLIGEVDPIR
                     VAEGRYLSDELTLHY"
BASE COUNT          555 a         1184 c         1183 g          462 t
ORIGIN      
        1 cctggctgcg caagtcgagt ggcgggcggt cgccgcgccg atcggcagtc taggggtccg
       61 gcgccgcgct cgtcaacccc gtcggatgac ggttcggaca cgcgacacat gacgtagcgc
      121 caatcccgtg acgcccagcg gcgcgtcgcg tgccgtcctc gcgcatctga tgcgccaatt
      181 gcggcattgc gttcatcaga tgaacctacg cgacggactg gacctccccg agtctcgacc
      241 cactcccgaa tcggccggat tcggtccggg tccgtgcatc tgatgcgcca gatcggacgc
      301 tcgatcgccc agaaggctcc aggcacgtcg gataggttca tcgggtgcac atcggtgtgt
      361 ggggctcagg cgtcgcggcg gacgatgacc cggcggaccg tcgcctgcgg cgaatcgatc
      421 tcgtggacga cccggaagcc ggctgccagg aaggtcgaca ggtggccggt gtagccgagg
      481 gcagcgggaa cccggccgtc gcccgcgtcg acggggtacg cctcgagacc cggggcgccg
      541 tgcgcccggg cgtactccat ggcggcgtcg aggaggcgcg tcgtcaggcc ctggccgcgg
      601 acggtccggg agacgacgaa gcacacgatc gaccagacgg gcacgtcggg agtcgccagt
      661 acatgcacca gcatgttttc gggtcgccgc cctcgccgaa cagcgcggcg acgtcgtccc
      721 agcggtccgg tgtcagggga acgatgctga tcgaggcggg gacgtcactc tctgtcatgt
      781 cgtggatggt actcagccgg tcggtctggg cctgtcatct ttggcgcggg catcgcgtcg
      841 tctcccacgt cgacggtttc gggaggtccc gcgagcgagg ccacgaggcg catgaccagc
      901 ggttggatca accggcgacc ggcctcgagg acgatggcga tcggtgcggc atgggtcgcc
      961 ggcagggcca ggaagaccgc caggaacggc gacatcaccg gcagcgccat cgtcgagtaa
     1021 tggaactggc tcgctggcca gacggcagga accgccagcc agccggcagt tcgtcgatcg
     1081 cgcagagcca ggagcaccag ggcaagcacg gtcacggcca acagctcggg gtagtagaag
     1141 gccgatccgc ccttggtcga ctcgtccatc aggcggcgcg agatgaaccc gagcttgccg
     1201 atatagtcga tccacaggtc ggaggcgatg acggccgtgg ctcctacgag gccaacaccg
     1261 gcccaggcgt gccgccagcg cccctcgccc accagcggga tgaatgcata caccttgagg
     1321 ccggtcgcga cggccgaggc gagcggatgg tttgccacca gtagggcaag gaccacgatc
     1381 tgggggttgg ccgagaagag cgcctccccg atcggcggga agagcagcca ccacagcggc
     1441 aggttcagac gacggagcgt ccagaccgcg gacgcccagg tggcgaggac ccagatccgg
     1501 gtgaatgtca cctcgctcac gagagccgcc ggggccatca ggacggtcgt cacgggcgag
     1561 cccgcgtagt ggaaggacca gccattgacg acgacggcgg catcccatgg atcgccaccg
     1621 tccagccagg cgacgacgcc ccgatagtag atcgtggcat cgatcccgac cgggaagccc
     1681 tggttggtga agaacgtcac ccggtcgtat gtctgccacg cgaaccagac gaccagcagg
     1741 aacgttccga cccaccggat tgccggaagg agggcgcgca catcctcgtc gacgagccgg
     1801 aacagccgac ggccgtgggc gaaccacccg gagtcacgca tcaccgcgaa tgctgcggtg
     1861 agccgttcgt ccggtcaacc caacgatggc atcatcactg gcgatggacg gcaaccgggc
     1921 gagccctcga acgcaagtgg cgccgccgcg ccgctccatt gtcgagctga tgatcgacgg
     1981 cgtcctcgac gcggagctgg ccggcctcgc ctggctgctc gtcgaggcgc gcctgccgct
     2041 gatcgtggct ggtctgcacc gcggcgccgg caagacgacg ctgttcgagg ccctcctcga
     2101 tttcctgcca tcgaccgctc gacggatcga cctcgcgggc gcgaccgagg agttcgcgtg
     2161 gctacccgag gcaggggcgc tcggctggcc cgcagctggc gagcgggccg agccgtcgtc
     2221 gatcccgatc acgcccgcca caggcttcct cgtcgccgcc gagctgtcgg agcacctgcc
     2281 gacccacacc tggggtcggg cggcgcggac gttggttcgg gcggcgtcga tcggctacgg
     2341 gatcggagcg acggttcacg ccgatcgcct cgaggacgtc cacgatctgc tgcgcggcag
     2401 cccggtcggc ctgacggacg acgagctgac ctacctcggt ctgatcctga tcgtccgggc
     2461 aaccagcgag ccggacggct cggtccgtcg ccgggtcgtc gccgcccact acgctcgacc
     2521 ggtgagtcgc gacgagcacg gccacaccca gcgaccggcg ccggcggtcc tcgcgtcccg
     2581 cgacgaggga gcggatcacc tggagcactt cgcctggggg gtgatgcccg agctcgcgat
     2641 ccggctcggc cggaaggccg gcgacatcga gcgggatcag ttcgagcgag cggcattgct
     2701 gaccgccctg gcccaggacc gcacgaccgg tgtcgacgag gtccgggcgg cgatccgcga
     2761 tgcccgccgg ctcccgacac cagcgatgga ccacctccac tgagacgttc ggcacctgac
     2821 gtttgacggt ccgcggagcc ggtcgtacgc tcgccgcccg tgccatcccc gtccgtcgat
     2881 cgccaacgcc gacccgagac cctcggcttg ctgcgcgccg cgggctggtc gagccggacc
     2941 gtcaagcagg agctccggtc caacctcctc gcccggctgg cggccgatcg gccgatcgtc
     3001 agcgggatcg tgggctatga cgagtcggtc ctgcccgcca tcgagaacgc ggtcgtcgcc
     3061 ggccaggacc tcgtcctcct cggcgagcgc ggccaggcca agacccggct cgcgcggctg
     3121 ctcgtcggcc tgctcgacga cgcgatcccg atcgtccgcg gcggcgagct gaacgacgat
     3181 ccgtacgcgc cgatcagccc gagcggcaag gcgatcgtcg ccgagcacgg cgacgccacg
     3241 ccgatcgact ggctgcctcg cgaccggcgc tacagcgaga agctcgcgac gccggacatc
     3301 acgatcgccg acctgatcgg cgaggtcgac ccgatccggg tcgccgaggg ccgctacctc
     3361 agcgacgagc tgaccctcca ctac
//
LOCUS       DATAIW010000327         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_247325, whole genome shotgun sequence.
ACCESSION   DATAIW010000327 DATAIW010000000
VERSION     DATAIW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247325"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1128)
                     /gene="dnaJ"
                     /locus_tag="VHM48_10035"
     CDS             complement(7..1128)
                     /gene="dnaJ"
                     /locus_tag="VHM48_10035"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2755793.1"
                     /translation="MATELDYYTTLGVARTATDAEIKKAFRKLAQQWHPDVNTEPVAQ
                     ERFKEINEAYQVLSDAQRRQTYDMFGKAGLGGDGGAGFDPSGFGSFSDIFDAFFGGSA
                     AAGARRGHPTAGSDLRYDIRITFEEAVRGADKEIEFPVYGRCETCTGTGAKPGTQPVA
                     CDNCGGRGEIRSTRRTMLGQMVNVTACPKCHGEGKLVTEPCEACHGEGRTERKRTLRV
                     SIPAGIDEGHQIRLSNEGEVGVRGGPAGSLYVAVHVTPHPKLRRDGTELSYEADISIA
                     QAALGTTIQVPTVDGQEPLEIKPGTQPGTEIRLRGRGVPHLRRTGSKGDLHVFVNVVV
                     PAKLTKRQRELLAEYAADAGESVAPPHGGILDKVRDALG"
     gene            1181..2032
                     /locus_tag="VHM48_10040"
     CDS             1181..2032
                     /locus_tag="VHM48_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX2755794.1"
                     /translation="MMPADSTWPPRLTVALTFDHDSISDGVRRGDTPVKLSHAEFGVR
                     VGAPRILALLASRGIVSTWFVPGHTLETFPESTAAILAGGHEIGCHGWFHEDCATLGR
                     DEERELVERCAVAVELATGVRPKGWRAPYWSLGEASLGIVEAAGFAYDSSLMADDFRL
                     YRVRQGDRHSVAEGSRFGREGALVEVPVSWALDDWPHFEPSSTGDRDGLSAPSKVLEI
                     WTEELRYAWAHAPGSLLTITMHPECIGRGHRMAMLERFIDAAAALDGVVFDRLDHYVA
                     NWQTRGS"
     gene            complement(2544..>2926)
                     /locus_tag="VHM48_10045"
     CDS             complement(2544..>2926)
                     /locus_tag="VHM48_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX2755795.1"
                     /translation="ALRSAYIASAVRCAPPANKPTPEERATCQPFLVRGLALLADVRV
                     LVALGAIAWESAFRTIAALTGEDARPRPKFLHGAVARVGGYVVVGSYHPSQQNVFTGR
                     LTAPMCKAVLDRACQLVAEVGTVD"
BASE COUNT          410 a          982 c         1062 g          472 t
ORIGIN      
        1 gaggtttcag ccaagcgcgt cgcggacctt gtcgaggatc ccgccatggg gcggcgcgac
       61 ggactcgccg gcgtcggcgg cgtactcggc gaggagctcc cgctggcgct tcgtgagctt
      121 cgccgggacg acgacattga cgaagacgtg gagatcgccc ttcgagccgg tccggcggag
      181 gtgcggcacg ccgcggccgc ggaggcggat ctcggtgccg ggctgggtgc ccggcttgat
      241 ctcgagcggt tcctggccgt cgacggtcgg cacctggatc gtcgtcccga gcgccgcctg
      301 ggcgatcgag atgtcggcct cgtacgagag ctcggtgccg tcacgtcgga gcttcggatg
      361 cggggtgaca tggaccgcga cgtagagcga gcccgccggt ccgccgcgga cgccgacctc
      421 gccctcgttc gagagccgga tctggtggcc ctcgtcgatg ccagccggga tcgacacccg
      481 gagggtccgc ttgcgctcgg tccggccctc gccgtggcag gcctcgcagg gctcggtgac
      541 gagcttgccc tcgccgtggc acttcgggca ggccgtgacg ttgaccatct ggccgagcat
      601 cgtccggcgg gtcgaccgga tctcgccgcg gccaccgcag ttgtcgcagg cgaccggctg
      661 ggttccgggc ttggcgcccg tgccggtgca cgtctcgcag cggccgtaga ccgggaactc
      721 gatctccttg tcggcgccgc ggacggcttc ctcgaacgtg atccggatgt cgtaccggag
      781 atccgagccg gcggtcgggt ggccgcgccg cgcgccggcg gccgcgctgc cgccgaagaa
      841 cgcgtcgaag atgtcgctga acgagccgaa gccgctcggg tcgaagccgg caccgccatc
      901 cccgccgaga cccgccttgc cgaacatgtc gtaggtctgg cggcgctggg catccgacag
      961 gacctggtag gcctcgttga tctccttgaa ccgctcctgg gcgaccggtt ccgtgttgac
     1021 gtccgggtgc cattgctggg cgagcttgcg gaacgccttc ttgatctcgg cgtcggtcgc
     1081 ggttcgcgcg acgccgagcg tggtgtagta gtcgagttcg gtggccatct gggcgggaag
     1141 tctacgggag gaccgtcggt cgggtggcta cgatgagtcg atgatgcctg ccgactccac
     1201 ctggcctcct cgtctgaccg tcgccctcac cttcgatcac gactcgatct cggacggcgt
     1261 ccgtcgcggc gacacgccgg tcaagctgtc gcacgcggag ttcggggtgc gggtcggggc
     1321 gccgcggatc ctggccctgc tcgcttcgcg cgggatcgtc tccacgtggt tcgtgccagg
     1381 gcacaccctg gagaccttcc cggagtcgac ggcggcgatc ctcgcgggcg ggcacgagat
     1441 cggctgtcac ggctggttcc acgaggattg cgcgacgctc gggcgggacg aggagcgcga
     1501 gctggtcgag cggtgcgcgg tcgcggtgga gctcgcgacc ggcgttcgac cgaagggctg
     1561 gcgggcgccc tactggtcgc tcggcgaggc ctcgctgggg atcgtcgagg cggccggctt
     1621 cgcctacgac tcgtcgctga tggcagacga cttccggctc taccgcgtgc gacagggcga
     1681 ccggcattcc gtcgccgagg gatcgcggtt cgggcgcgag ggcgcgctcg tcgaggtgcc
     1741 ggtctcctgg gcgctcgacg actggccgca tttcgagccg tcgtcgacgg gcgatcgcga
     1801 tgggctatcg gcgccgtcga aggtgctcga gatctggacg gaggagctgc gctacgcgtg
     1861 ggcccacgcc ccaggcagcc tcctgacgat cacgatgcac cccgagtgca tcggacgcgg
     1921 ccaccggatg gcgatgctgg aacggttcat cgacgcggcc gccgcgctcg acggcgtcgt
     1981 cttcgaccgc ctcgaccact acgtggcgaa ctggcagacg cgagggtcgt aagtcgcggc
     2041 gttctggacg ggcggtcgtt gcgctggcga cgacgccggt aggtcgcgtt cattcaatga
     2101 accgatgcga cgaggttgcc cgcgatcgct cgtgaattcg cttggaacgg atcatcaaac
     2161 gagcggaatc ggcaagccga gcctctcgag cgtgccgcgg accgcgtctg actccgaatc
     2221 gtggccaacg acgtcgcatt ggttcatctg atgaacgcga ttcacgggct cgtgcgtctg
     2281 atgcgccatc gcggatcgat cggcggtcca aggtcgaacc acgcgcctca accggcgcga
     2341 cggagtcgat cggcgttgcc gaagttgccg aagcgagcgg acttgttacc agcatggcgc
     2401 agaggccatg cccaagggct ggcttcgcag gttcgaaaac ggtcggcaac gccgcgcttc
     2461 tgccgagcgg ccgaggcaag ctctgaggcg accacgcctc gaactcggac gcgacccgac
     2521 agacgggtcc ttcgttgctc cgcctagtcg acggtcccga cctcggccac aagctggcat
     2581 gcccggtcga ggaccgcttt gcacatcggt gccgtgagtc ggcccgtgaa gacattctgt
     2641 tgtgacgggt ggtacgagcc cacgacgaca tacccgccga cgcgcgccac cgccccgtgc
     2701 aggaacttcg gtcggggccg cgcgtcctca ccagtcagcg cggcgatcgt ccggaacgcc
     2761 gactcccacg cgatcgcgcc gagcgcgacc agcacccgga cgtccgcgag gagcgcaagc
     2821 ccccggacga ggaacggctg gcaggtcgcc cgctcctcgg gcgtcggctt gttcgccggc
     2881 ggtgcgcagc ggaccgccga cgcgatgtag gcgctacgca acgcca
//
LOCUS       DATAIW010000328        11717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_128229, whole genome shotgun sequence.
ACCESSION   DATAIW010000328 DATAIW010000000
VERSION     DATAIW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128229"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VHM48_10050"
     CDS             <1..613
                     /locus_tag="VHM48_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2755796.1"
                     /translation="RAEEFYVDQLGFVVEQEMLPAFARIRRDDLTLWLAGPASSAARP
                     MPDGGQPGPGGWNRFVIEVDDLAAVVESLGAAGAVFRNDVVNGPGGRQILVEDPDGDP
                     ARIEANVAAMTATALALHRRGHLPVMGEWFALPLIEAAESAGDDPDDAFDAIFHPIAE
                     QVLARCDACLRIGGPSEGADRMVATARRLGKAVFTGLDEVPAP"
     gene            795..1586
                     /locus_tag="VHM48_10055"
     CDS             795..1586
                     /locus_tag="VHM48_10055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX2755797.1"
                     /translation="MSEAISEATFSKSEMMIVAAARELAGQRVCFVGVGLPNIAVNLA
                     QRTVAPELELVYEAGVFGARPARLPLSIGDPTIVTGATAATSMFELFAFYLQGGLIDV
                     GFLGAAQIDRFGNINTTVIGDYDHPKTRLPGSGGACEIAINARQVFVIMRQSARSFVE
                     TIDFRTSPGNLGGAEQSERIRREQGWLGRGPSVVVTDLGIYHFDETGEMRLDSLHPGA
                     TVEQVRATVGWDVRIAANVAATPPPTADELRLIRDELDPGGAYTK"
     gene            complement(1743..3104)
                     /locus_tag="VHM48_10060"
     CDS             complement(1743..3104)
                     /locus_tag="VHM48_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755798.1"
                     /translation="MRTDPLPRLVLIGLTTLSIIACSSAAMPPTPASPSSSSPSSPSS
                     PSSMPGTSEEPAVAWLLVGRRGEPDLHLVLATTGEVAMDIPDGSPRSHWDRIVTATAD
                     GAATIVRDIVVQPGFGGPELRIDGHWRLPTVGLDPIPAGRSLDGSTIALVERAYDPSA
                     GVSRFAIVDHFLLDAVQTAGDAPLRLVRVIELRGAFEYDTLSPDGRILYVVQHLDGEA
                     GGHYQVRAVDVPTGLMRNGVIVDKTHPDETMAGSPVAQVRRPDGLVLTLYRGPEHPFI
                     HALASMEAWAVCIDLPVGDAHNPDAGLDWGLAPSADGSSVFAVNASLRLVVEIDPTGL
                     AVRRSASIGSSAAAPIVLAKFGHGDVGPVGRRLVVSPDGTMLFAAGADGISAIRTRDL
                     AAVRHDLAGSAIDSVGITPDGRVLFALVRGGTIVALDAATGRDLGTVPGNDYDRLLAV
                     APW"
     gene            complement(3184..4077)
                     /locus_tag="VHM48_10065"
     CDS             complement(3184..4077)
                     /locus_tag="VHM48_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755799.1"
                     /translation="MTDPDLEGALRDLGGSLATPFIAGGAGVLDPARRARLRIERGAA
                     SVRRQPRWAGLVPSPRRPFGRGVGLGLVLLVVLAAVAGAIGLGLPGIRILQVPSGSAV
                     ATAVAPSGSPTFSAPSRSPGTSASPAPGSPFPAGAGTLGGGLGLGDPIAVSGASSAVD
                     IPVLLPSAQGVGSPVSAWLLDGRLALVWRVSSALPATREPGVGLILSEFRGSLEPGYF
                     EKIIGPDTTVDPVTVDGVTGYWISGAPHEIVFVGTDGQPVFDSRRIVGDTLIWARGDV
                     TYRLESGLDRAAAIALAETLR"
     gene            complement(4094..4606)
                     /locus_tag="VHM48_10070"
     CDS             complement(4094..4606)
                     /locus_tag="VHM48_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2755800.1"
                     /translation="MAYEDLVRRHQAVAVRTAHLFAPDGDAEDAVQEAFVKAHAALGR
                     FRAGAPFRPWLLRIVANEARNRRRSAGRRAGLVLRSVEDRRPDDAAPSPESAILVDER
                     RLWLLAGVNALRDEDRDVIAARYFLDLSEAETSEILGLPRGTVKSRLSRGLVRLREQL
                     AATDEGSPHG"
     gene            4893..6236
                     /locus_tag="VHM48_10075"
     CDS             4893..6236
                     /locus_tag="VHM48_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2755801.1"
                     /translation="MTGRVFRRSLDPALPVAVEAFGSTIRDTAGREYLDAAGGAIVVN
                     VGHGRASVARVLGEQSARLSYAHGSAFTTEPLEAYAAEIGPLLPVDDPAIYPVSGGSE
                     AIETALKLARAYHLARGETERWIVYARWGSYHGNTLGALDLSGRRPLRRPYEGWLGRF
                     RHVSAAYPYRGNDPDSQALATADELAAELDRAFSAAEPGSVAAFVAEPIVGATLAAAV
                     PPDDYWPKIADVCRRHGVLLIADEVMTGFGRTGRWFGLDHWGVRADLLVAAKGATSGY
                     WPFGFVAAAGPIHDTVTGHGGFVHGFTYSHAPAGAAVAREVLRILEDEDLVEASRVKG
                     DRLRNLLIDRLGGHEHVGEIRGRGLMVGIELVADRETRRAFPRAARLTETVVRAARDA
                     GVLVYSGTGNANGVDGDTILLGPPFVVTDAELERIVDTVATAIEAAAVDVATSTG"
     gene            complement(6648..7175)
                     /locus_tag="VHM48_10080"
     CDS             complement(6648..7175)
                     /locus_tag="VHM48_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755802.1"
                     /translation="MNDRNVSDNSGVRAWAWRWGPDEDRRPGLPWIGIFLVVFGLLLL
                     VERMLPGQRLGDVAVLAAGLASLAAWILRRGTIALYAGAFLTALALPGTIQALGQPLG
                     PGWGTLFFGLAFLLVAAVRASQGGGVGWQALYGGILVLIGGSEVVKPDVADVAWPLIL
                     VAVGLLLLVRGGRPG"
     gene            complement(7546..9918)
                     /locus_tag="VHM48_10085"
     CDS             complement(7546..9918)
                     /locus_tag="VHM48_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2755803.1"
                     /translation="MLFSRWGAFVYRFRRPIALITVIVAIASVTLASRVTDALVSGGW
                     SDPHSDSALVSTRLADEFGVGRGSIVALFQGDAATDARSTTFQQMIATSLGRLAADPL
                     ADDVIGFAQTQDARFISTDGHAAYAVVRLTITDEQSANEMPKIRGLIDKPSGATLELA
                     GVGPFTEDQAHQSEKELVQAETVSAPFLLLILLAVFASVVAAGLPLLVAALAIPTTLA
                     GVYLVAQTTELSIYVQNVATMLGLALAIDYSLFMVSRFREELRRGRTVAQAVETTVAT
                     SGKAVTFSGLAVAIGLSGLLLFQPAALRSFGIGGSIVVASSVFYALTFLPAVLGMLGP
                     RVNALSLAGLFAAIGRRLGRPARPVSAAPDRSRWAGVARAVMARPLAFLIPAVLVLLL
                     AGTPFLRLSQGIPNAATLPAGLESRDAAVTLATDFQAGETSPIAILATVDGDPLTAAN
                     IARLTQFASLVDGVAGIDRIEGPFSLVDPQTGVVMSPDQLAALYAAPADQLPAQLVAA
                     RDGLKQAYVRGSTVRLDAVSPLDPVSPAGGAVIPAVRAIHVEGVTSAVGGLAAEGHDF
                     MVSQGNTVPWAVAVTLFASGAILFLLFGSVVIPAKAIVMTLLSITASFGALVWIFQEG
                     NLSDVLGFTSPGFTVAGNPIIMFSILFGLSMDYEVLLLSRIQEAYRRTADNTASVAEG
                     LSRTAGVITGAALIMVTVFSAFALAESITIKSIGVGMAIAVAVDATIIRVLLVPATMR
                     LLGRWNWWAPGPMGRLADRLGFSHVEDDPDAADGGEKGDAGRRSTGLAGS"
     gene            10072..10764
                     /locus_tag="VHM48_10090"
     CDS             10072..10764
                     /locus_tag="VHM48_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2755804.1"
                     /translation="MPRVTAKHEQEVRDRIVRAALAVFAKHGFHRATMQDIVRASGLS
                     VGAIYTYFKSKSDLILAGCDLITDQELAQLGGRLAAVTGYRDRIAAAIGFWFDNLAVE
                     QSERGSGFLILQAWAEADTDPAIREMLLRRRRETVTAVVLVLQEGVMRAELPAWLDIP
                     SVGHALAALLDGMLIEAAEDGGAYRRSDAERRVLALLETLLAAKDAAPPERITPAIAQ
                     PYESARAGRVAS"
     gene            10761..>11717
                     /locus_tag="VHM48_10095"
     CDS             10761..>11717
                     /locus_tag="VHM48_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755805.1"
                     /translation="MTDAGSSTGSSSVGRVQTVLGPIEPADLGWVLPHEHTAIALWHI
                     PNRWDYWELRRDEPIIVEELAAFRAAGGGTLVDVTLDGVGRDPEWLAAISRTTGLHLV
                     MGAGWYRGAHYPAEALIDRRSVDALAAVIVRDAMVGVGETGIRAGIIGEIGTDKPWLS
                     AQEERVHRACARAARRTGLAITTHAVQSRVGLDQLDVLEAEGADLSRVVIGHADSNPS
                     LDYHLAIVGRGATVEFDFLGMSFTPLERHGESRIVDNLRELLARGHVSRILLSQDVCH
                     DSQLRRYGGNGYTYLADTFLPRLRAAGVSEDEIRTITVDNPRR"
BASE COUNT         1759 a         4166 c         4232 g         1560 t
ORIGIN      
        1 ccgggccgag gagttctacg tcgaccagct cggcttcgtg gtcgagcagg agatgctgcc
       61 ggcgttcgcc cggatccgcc gcgacgacct gacgctgtgg ctcgccggtc cggcctcgtc
      121 cgcggcgcga ccgatgcccg acggcggcca gcccggaccc ggcggctgga accggttcgt
      181 gatcgaggtc gacgacctcg ccgcggtcgt ggagagtctc ggtgccgccg gggccgtgtt
      241 ccgcaacgat gtcgtcaacg gaccgggcgg caggcagatc ctcgtcgagg acccggacgg
      301 cgatcccgct cggatcgagg ccaacgtcgc ggcaatgacg gcgacggccc tcgccctcca
      361 ccgtcgcggg cacctgccag tgatggggga gtggttcgcg ctgccgctga tcgaggcggc
      421 cgagtccgcc ggcgacgacc cggacgacgc gttcgacgcg atcttccacc caatcgccga
      481 acaggtcctg gcccgctgcg acgcctgcct ccggatcggc ggcccgtccg aaggcgcgga
      541 ccggatggtc gcgacggccc gccgcctcgg caaggccgtg ttcaccggcc tcgacgaggt
      601 cccggcgcca tgaccccggc tggttcggcg acggctgcgc acatggtgcg cgctacgacg
      661 gctggcgtcg tcgccgaacg tcagcatgcg cgccgcgcga gcaaaacgat gccggacgac
      721 gcgatccggc cgctcggcgt cgagctcctt cgtcacagtg cgcacgcggc gcgcacggat
      781 cggccggcgc ggcgatgagc gaggcgatca gtgaggcgac cttcagcaag tccgagatga
      841 tgatcgtcgc ggcggcccgc gagctcgccg gccagcgcgt ctgcttcgtc ggtgtcgggc
      901 tcccgaacat cgcggtcaac ctcgcccagc ggaccgtcgc ccctgagctc gagctcgtct
      961 acgaggccgg cgtcttcggt gccaggccgg cccgcctgcc gctgagcatc ggcgatccga
     1021 cgatcgtcac cggcgccaca gccgccacga gcatgttcga gctcttcgcc ttctacctgc
     1081 agggcgggct gatcgacgtc gggttcctcg gcgcggccca gatcgaccgg ttcggcaaca
     1141 tcaacacgac cgtcatcggc gactacgacc acccgaagac gcggctgccg gggtccggcg
     1201 gcgcctgcga gatcgcgatc aacgcccgcc aagtgttcgt gatcatgcgc cagagcgcac
     1261 ggagcttcgt ggagacgatc gacttccgga cgagccccgg caatctcggc ggggccgaac
     1321 agagcgagcg catccggcgc gagcaaggct ggctcgggcg tggcccgagc gtcgtcgtga
     1381 ccgacctcgg gatctaccac ttcgacgaga ccggcgagat gcggctcgat tcgctccacc
     1441 cgggagcgac cgtcgagcag gtccgcgcga cggtcggctg ggacgtcagg atcgcggcca
     1501 acgtcgctgc gacaccgccg ccaaccgcgg acgaactccg cctgatccgc gacgagctcg
     1561 atccgggcgg cgcgtacacg aaatagcgcc gcggtccgcc gcagcgctca cgtccccggt
     1621 ggtcgaccgg ggttcaagat gggtgtcggg catcccgttc cggcacccat ctagggcacg
     1681 atcatcgggc gttcggacct tccgcggagg ggccgaacgg cccgttcggg gagggccatt
     1741 tctcaccacg gggctaccgc gagcagccgg tcgtagtcgt tgcccgggac ggtccccagg
     1801 tcgcgcccgg tggcggcgtc gagagcgacg atcgtgccgc cgcggacgag agcgaacaac
     1861 accctgccgt cgggcgtgat ccccaccgaa tcgatcgccg acccggcgag gtcatgacgc
     1921 acggccgcga gatcgcgggt ccggatcgcg ctgatgccgt ccgcacccgc ggcgaacagc
     1981 atcgtgccgt cgggcgacac gaccaggcgt cgcccgactg gcccgacgtc gccatgaccg
     2041 aacttggcga ggacgatcgg cgcggcggcg ctcgacccga tcgacgcgga ccggcgcacc
     2101 gcaagcccgg tggggtcgat ctcgaccacg aggcggaggc tcgcgttgac cgcgaacacg
     2161 gacgagccgt ctgcgctcgg ggccagcccc cagtccaggc cggcgtccgg attgtgggcg
     2221 tcgccgaccg gcaggtcgat gcagacggcc cacgcctcca ttgaggcgag ggcgtggatg
     2281 aacggatgct ccggtccccg gtagagcgtc aggacgagcc cgtccgggcg ccgcacctgg
     2341 gcgaccggcg agcccgccat cgtctcgtcg gggtgggtct tgtcgacgat gaccccgttg
     2401 cgcatcagcc cggtagggac atcgacggcc cgcacctggt agtggccgcc cgcctcacca
     2461 tcgagatgct ggacgacgta gagaatccgg ccgtcgggcg agagggtgtc gtattcgaag
     2521 gcgccgcgca actcgatcac gcggacgagt cggagaggcg cgtcgccggc ggtctggacc
     2581 gcatcgagga ggaagtggtc gacgatcgcg aagcggctca cgccggccga agggtcgtag
     2641 gcacgctcga cgagggcgat cgtcgaaccg tcgagagacc gaccggccgg gatcgggtcg
     2701 agcccgaccg tcggcaggcg ccagtggccg tcgatccgca gctccggtcc gccgaagccg
     2761 ggttggacga cgatgtcccg gacgatcgtc gccgctccat cggccgtcgc ggtgacgatg
     2821 cggtcccagt gggatctcgg gctgccgtcg ggaatgtcca tcgcgacctc accggtcgtg
     2881 gccaggacca gatggaggtc cggctcgccg cgccgaccca ccaggagcca ggctacggcc
     2941 ggctcctcgc tggtccccgg catcgaggac ggcgaagacg gcgatgacgg cgatgacgac
     3001 gacggagacg ccggggtcgg cggcattgcg gcgctgctgc aggcgatgat cgacaaggtc
     3061 gtgaggccga tcagcacgag gcgcggcaga ggatcggtgc gcatcggtct cgactccttc
     3121 gtccggcgga cccgccgtcg ggtcgttacc cggtctacac cgcgagcgcc gggccggttc
     3181 ctgtcagcgg agcgtttcgg cgagggcgat cgctgccgcg cggtccagcc ccgactcgag
     3241 ccggtacgtc acgtcgccgc gcgcccagat gagcgtgtca ccgacgatcc gccggctgtc
     3301 gaagacgggc tgcccgtccg tcccgacgaa cacgatctcg tgcggcgcgc cgctgatcca
     3361 gtagccggtg acgccatcga ccgtaaccgg gtcgacggtc gtgtcggggc cgatgatctt
     3421 ctcgaagtag ccgggctcga gcgagccgcg gaattcggag aggatgagtc cgacgcccgg
     3481 ctcgcgggtc gctggcaggg cactgctgac cctccagacc agcgccaacc tgccgtcgag
     3541 cagccacgcg ctgaccggtg atccgacgcc ctgcgcagat ggcagaagca cggggatgtc
     3601 gaccgcggac gaggcgccgg aaacggcgat cgggtcgccg aggcccagtc cgccgccgag
     3661 cgttccggca cccgccggga aggggcttcc aggggccgga ctcgcggatg tgcccggtga
     3721 ccgcgacggc gcgctgaagg tcggcgatcc gcttggggca acagccgtgg cgacggccga
     3781 tccgctcggc acctgcagga tccggatccc gggcagcccg agcccgatcg ccccggcgac
     3841 cgcggcgagg acgacgagca gaaccagccc aagcccgacc ccgcgcccga acgggcggcg
     3901 cgggctcggc acgagcccgg cccaacgtgg ctgccggcga acggatgccg cgccgcgctc
     3961 gattcgcaga cgcgcccggc gcgccggatc gaggacgccc gcgcccccgg cgatgaacgg
     4021 tgtcgccagc gacccgccga ggtcgcgaag cgcgccctcg aggtcgggat cggtcatcga
     4081 catgcgggcc cggtcaacca tggggcgacc cttcgtcggt cgccgcgagc tgctcgcgga
     4141 gccggaccaa tccgcgcgac aggcgcgact tcaccgtccc gcggggcagc ccgaggatct
     4201 cggaggtctc ggcctccgaa aggtcgagga agtagcgggc cgcgatcacg tcacggtcct
     4261 cgtcgcggag ggcgttgacc ccggcgagga gccagagccg ccgctcgtcg acgaggatcg
     4321 ccgactcggg cgatggggcc gcatcgtccg gccggcgatc ctcgacggag cggaggacca
     4381 ggccggcgcg acggccggcc gatctccgtc gattgcgggc ctcattggcc acgatccgga
     4441 gcagccacgg ccggaatggc gcaccggccc ggaagcggcc cagcgccgca tgggccttga
     4501 cgaaggcctc ctggacggcg tcctcggcgt cgccgtcggg cgcgaagagg tgcgcggtcc
     4561 ggacggcgac agcctgatgc cggcggacga ggtcctcgta ggccatgacg tcgcctttcc
     4621 gtgcgcggtc gatcagctcg gcgtcgcgac gcccgtcgtc ttccagtgga cggccctcca
     4681 cagtcaaccg tactgctaca ccggccggcc gccgatggtt ccctgcgcct cgagccgcgg
     4741 gggcgggcat gatccttcac gcgctgcccg gggcgcggtc ggccgctgac tcacgggcgg
     4801 cccggctgcc ggcgcgtcgg gtcgagcgcc gcccgaggcc gatccgctgc tacgatcgac
     4861 ccgatgaccc cgtcctctcg accccttgtc gcgtgaccgg tcgcgtcttt cggcgctcgc
     4921 tcgatcccgc cctgccggtc gcggtcgagg cattcggctc gacgatccgc gacaccgccg
     4981 gtcgcgagta cctcgacgcc gccggcggtg cgatcgtcgt caacgtcggc cacggtcgcg
     5041 cgtcggtcgc ccgcgtcctc ggggagcagt cagcccgcct gtcctatgcc cacggctcgg
     5101 cattcacgac cgagccgctc gaggcgtatg ccgcggagat cgggccgctg ctgccggtcg
     5161 acgaccccgc gatctatccg gtcagcggcg gctccgaggc gatcgagacc gccctgaagc
     5221 tcgcccgggc ctaccacctc gcccggggcg agacggaacg ctggatcgtc tatgcccgct
     5281 ggggcagcta ccacggcaac acgctcggcg cgctcgacct gtcgggccgg cgacccctcc
     5341 gccgaccgta cgagggctgg ctcggccggt tccgccacgt ctcggctgcc tacccgtacc
     5401 gcggcaacga tcccgacagc caggcgctcg cgaccgccga cgagctcgcc gccgagctcg
     5461 atcgggcctt ctccgcggca gagccgggct ccgtcgcggc cttcgtcgcc gagccgatcg
     5521 tcggcgccac gcttgccgcc gccgttccgc cggacgacta ctggccgaag atcgccgatg
     5581 tctgccgtcg ccacggggtc ctcctcatcg ccgacgaggt gatgaccggc ttcggacgga
     5641 ccgggcgctg gttcgggctc gatcactggg gcgtccgggc cgacctcctc gtcgccgcga
     5701 agggcgcgac ctcgggctac tggccgttcg ggttcgtcgc tgccgccggc ccgatccatg
     5761 acacggtcac cgggcacggc ggcttcgtcc acgggttcac ctattcgcac gccccggccg
     5821 gcgccgccgt cgcccgcgag gtcctccgga tcctcgagga cgaggacctc gtcgaggcga
     5881 gccgggtcaa gggcgatcgg ctccgcaacc tgctgatcga ccgcctcggc ggccacgagc
     5941 acgtcggcga gatccgcggc cgagggctga tggtcgggat cgagctggtc gcggatcgcg
     6001 agacgcgtcg tgcgtttccg cgcgccgccc gcctgaccga gaccgtcgtt cgcgcggccc
     6061 gcgatgcggg tgtcctcgtc tactccggca ccggcaacgc gaacggcgtc gacggcgaca
     6121 cgatcctgct cgggccgccg ttcgtcgtga ccgacgccga gctcgaacgg atcgtcgata
     6181 cggtcgcgac ggcgatcgag gcggcggccg tcgacgtggc gacctccacc ggctgagggc
     6241 gcaggcgcca accgcgggcc ccgtggcgag cgcacgtctc gcatcgaccc gttctgcgcg
     6301 gttcgcgagg acggatcaga cccggacccg ggggccagcc tcggacaagt ccatcgacgg
     6361 tggactgtcg aatgccatgg tggcaacatc agcatccacg gcagaccggg ccgcgcgacg
     6421 aggtcgactt ccgccttcac gccgaccggc cgaacgtgat cgtcgagagg gggaaggggc
     6481 tgggtcgcgt gaccagccgt tcgagtgacc gccgcggtgt cggttcgggt tcgcggcacg
     6541 cccaccgacc ctcgtggatc gggttcgcgc gggcgccgag ccggctgaac gagctgcaat
     6601 gaccggggcc gcggcgttga tcgagtgatc ggcggcggcc gatccggtca gccgggacgg
     6661 ccgccccgaa ccagcaggag caggccgacg gcgacgagga tcaacggcca cgcgacatcc
     6721 gcgacgtccg gcttgacgac ctccgagccg ccgatcagga cgaggatccc accgtacagc
     6781 gcctgccagc cgactccacc gccctgcgac gcacggaccg cggcgacgag caggaaggcg
     6841 agcccgaaga acagcgtgcc ccagccggga ccgagcggct gaccgagcgc ctggatcgtg
     6901 cccggcaggg cgagcgcggt caggaaggcg ccggcgtaga gggcgatcgt tccccgccgc
     6961 aggatccagg cggcgagcga ggcaagcccc gccgcgagca ccgcgacatc gccgagccgc
     7021 tggcccggca gcatccgctc gacgagcagc agcaatccga agacgacgag gaagatcccg
     7081 atccagggca gacctgggcg ccgatcctcg tccggtcccc agcgccacgc ccaggcccgg
     7141 acgcccgagt tgtccgacac gtttcgatca ttcatctgac cctcctccgt cgccacgccg
     7201 ctgagggaac ttgccggcca cggcagcagt gtctctgatc aacgagcctc atgggcggcc
     7261 gcgggggaag tccaggagct cccattgcag cgtggcggtc agccacgcca gatatcgctc
     7321 cgacgtccag cctcggccga tcacgagaag atcgaagacg gccagtgagc aatacgtcca
     7381 gatcaggtcg cgcgcctcct cgactgaaag gccgggtcga aggacgcccc gctccgcgag
     7441 ccggctcgcg agccggccct ggcctgcgtg tcggctcgcc tcgatctcgc gccacagggt
     7501 tgccagctcg ggatcgatcg cggtcgctgg gagcggcccg cccggtcacg atccggcgag
     7561 gccggtcgac ctccggccgg cgtcgccctt ttcgcctcca tcggctgcgt ccggatcgtc
     7621 ctcgacgtgg ctgaagccga gccggtccgc gagccggccc atcgggcccg gcgcccacca
     7681 gttccagcgg cccagcagac gcatcgtggc cggaacgagg aggacccgga tgatcgtggc
     7741 gtcgaccgcg accgcgatcg ccatgccgac cccgatgctc ttgatcgtga tcgactcggc
     7801 cagcgcgaac gcggagaaga ccgtgaccat gatcagtgcg gcgccggtga tcacgccggc
     7861 cgtcctcgac agcccctcgg cgaccgaggc ggtgttatcg gcggtccgcc gatatgcctc
     7921 ctggatccgc gaaaggagca ggacctcgta gtccatcgac aggccgaaca ggatgctgaa
     7981 catgatgatc gggttgccgg ccacggtgaa gcccggcgag gtgaagccca ggacgtcgga
     8041 caggttgccc tcctggaaga tccagacaag ggcgccgaag ctggccgtga tcgacagcag
     8101 ggtcatcacg atggccttcg ccgggatcac gaccgagccg aagagcagga acaggatcgc
     8161 gccactcgcg aacagggtca ccgcaaccgc ccacgggacc gtgttgccct ggctgaccat
     8221 gaagtcgtgg ccctcggccg cgagtccgcc gaccgcgctc gtgacgcctt cgacatggat
     8281 ggcccggacc gccgggatga cggcgccgcc ggccgggctg accgggtcga gcgggctgac
     8341 ggcgtcgagt cgcacggtcg agccgcggac gtaggcctgt ttgagcccgt cgcgggccgc
     8401 gacgagttgt gccgggagct ggtcggccgg ggcggcgtac agggcggcga gctggtccgg
     8461 gctcatcacc accccggtct gcgggtcgac gaggctgaac gggccctcga tccggtcgat
     8521 gccggcgacg ccgtcgacca ggcttgcgaa ctgggtcagg cgagcgatgt tggccgccgt
     8581 cagcgggtcg ccgtcgaccg tggcgaggat cgcgatcggt gacgtctcgc cggcctggaa
     8641 gtcggttgcg agcgtgaccg cggcgtcgcg gctctcgagg ccggccggca gcgtcgctgc
     8701 gttcgggatg ccctggctga gacgcaggaa cggggtgccg gcgaggagga ggacgaggac
     8761 cgccgggatc aggaaggcga gtggacgggc catgaccgcg cgggcgacgc ccgcccagcg
     8821 cgagcggtcc ggtgccgccg acaccggcct cgccggccgg ccgagccgcc gcccgatcgc
     8881 cgcgaacagg ccggccaggc tcagcgcgtt gacccgcgga ccgagcatcc cgaggacggc
     8941 cggcaggaac gtcagcgcat agaagaccga cgaggccacg acgatcgagc cgccgatccc
     9001 gaacgagcga agggcggccg gctggaacag gagcaatccg gacagcccga tcgcgacggc
     9061 gagcccggag aaggtcacgg ccttgccgct ggtcgcgacc gtcgtctcga cggcctgggc
     9121 caccgtccgc ccgcggcgca gctcctcgcg gaagcggctg accatgaaca gcgagtagtc
     9181 gatcgcgagc gcaagcccga gcatcgtcgc cacgttctgg acgtagatcg agagctcggt
     9241 cgtctgggcg acgagataga cgccggcgag ggtcgtcggg atcgcgaggg cggcgacgag
     9301 cagcggcaga ccggcggcga cgaccgaggc gaagaccgcg agcaggatca gaaggaggaa
     9361 cggggccgag accgtctcgg cctggacgag ctccttctcc gactggtggg cctggtcctc
     9421 ggtgaacggg ccgacgccgg cgagctcgag ggtggccccg ctcggcttgt cgatcaggcc
     9481 gcggatcttc ggcatctcgt tggccgactg ctcgtcggtg atcgtgagcc ggacgaccgc
     9541 gtacgcggcg tgaccgtcgg tgctgatgaa gcgcgcatcc tgggtctggg caaagccgat
     9601 gacgtcgtcg gcgagcgggt cggcggccag gcgccccaag gatgtcgcga tcatctgctg
     9661 gaaggtggtt gagcgggcat ccgtcgcggc gtcgccctgg aacagcgcga cgatcgagcc
     9721 gcggccgacc ccgaactcgt cggccagccg ggtcgagacc agggccgagt cggaatgcgg
     9781 gtcggaccag ccgcccgaga ccagggcatc ggtcaccctg gacgcgagcg tgaccgacgc
     9841 gatggcgacg atcacggtga tcagggcgat cgggcggcgg aagcggtaga cgaatgcgcc
     9901 ccagcgggag aacagcatcg ggatgcccct tcgatcggac ggttgcggcc gcggcggcac
     9961 ggtggtgtgg gtccctcggg aacatagaga atgagcattc gttttgtcaa gacttgtcgt
    10021 tgataatcgt cctgagtacg ctaggggagc gaccattctc caggagccgc catgccccgt
    10081 gtcaccgcca agcacgagca ggaagtccgc gaccggatcg tccgggcggc tctcgccgtg
    10141 ttcgccaagc acggcttcca ccgggcgacg atgcaggaca tcgtccgcgc ttcgggtctg
    10201 tcggtcggcg ccatctacac gtacttcaag agcaagtcgg acctgatcct cgccggctgc
    10261 gacctgatca ccgaccagga gctcgcccag ctcggtggcc ggctggccgc ggtcaccggc
    10321 taccgcgacc ggatcgcggc ggcgatcggg ttctggttcg acaacctcgc ggtcgaacaa
    10381 tccgagcgcg gctcgggctt cctgatcctg caggcctggg ccgaggccga caccgatccg
    10441 gccatccgcg agatgctcct ccggcgccgt cgcgagaccg tcacggcggt ggtcctcgtc
    10501 ctccaggagg gcgtgatgcg ggccgagctg ccggcctggc tggacatccc gtcggtcggt
    10561 cacgccctgg ccgccctgct cgacgggatg ctgatcgagg ccgccgagga tggcggggcc
    10621 taccgccgct ccgacgccga acggcgggtc ctcgccctgc tcgagacgct gctggcggcg
    10681 aaggatgccg ccccgcccga gcggatcacg cctgccatcg cgcagccgta cgagtcggcc
    10741 cgggccggcc gggtcgcctc gtgaccgacg cggggtcgag caccggctcg tcctccgttg
    10801 ggcgcgtcca gaccgttctc gggccgatcg agccggcgga tctcggctgg gtgttgcccc
    10861 acgagcacac cgcgatcgcg ttgtggcaca tcccgaaccg ctgggactac tgggagctcc
    10921 gtcgcgacga gccgatcatc gtggaggagc tggcagcatt ccgggcggcc ggcggtggga
    10981 ccctcgtcga cgtgaccctc gatggggtcg ggcgcgaccc cgaatggctg gcggccatct
    11041 cccgcacgac gggactgcac ctcgtcatgg gcgcgggctg gtatcgcggc gcccactacc
    11101 cagccgaggc gctgatcgat cggcgatcgg tcgacgcgct tgccgccgtg atcgtccgcg
    11161 acgcgatggt cggggtgggc gagaccggga tccgggccgg gatcattggc gagatcggga
    11221 ccgacaagcc gtggctgtcg gcgcaggagg agcgggtcca tcgggcgtgc gcccgggccg
    11281 cccgccggac cgggctggcg atcacgaccc acgcggtcca gtccagggtc ggtctcgacc
    11341 agctcgacgt cctcgaggcg gagggcgcgg acctgtcgcg cgtcgtcatc ggccatgccg
    11401 actcgaaccc gtcgctggac taccacctgg cgatcgtcgg gcgcggcgcg accgtcgagt
    11461 tcgacttcct cgggatgagc ttcaccccgc tcgagcgtca cggcgagagc cggatcgtcg
    11521 acaatctccg cgagctgctc gcccgcggcc acgtgtcgcg gatcctcctg tcgcaggacg
    11581 tctgccacga cagccagctc cgccgctacg gcggcaacgg ctacacctac ctcgcggaca
    11641 ccttcctgcc gcggctccgg gccgccggcg tgtccgagga cgagatccgg acgatcacgg
    11701 tcgacaaccc gcgccgg
//
LOCUS       DATAIW010000329         6150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_110535, whole genome shotgun sequence.
ACCESSION   DATAIW010000329 DATAIW010000000
VERSION     DATAIW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110535"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHM48_10100"
     CDS             <1..222
                     /locus_tag="VHM48_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755806.1"
                     /translation="VLTAALAAAGFAPGASPQPVAAADSVAATTPEPTVQVDTVYVAA
                     PAKQQTITVHKVVKSTGGEESEGESGGDN"
     gene            295..690
                     /locus_tag="VHM48_10105"
     CDS             295..690
                     /locus_tag="VHM48_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755807.1"
                     /translation="MSRPLPPRPTGPRPVAAATPELPPRRAVPAVERKARPDPGPLRL
                     AFGLTGMAAATALLSAFLVPATSADAGAGTTNLVTVADGPTPSVQHVTRYVQLQPGQT
                     APPHAVVNQAPTPKPRIVVVTTRQSGAKP"
     gene            765..1748
                     /locus_tag="VHM48_10110"
     CDS             765..1748
                     /locus_tag="VHM48_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2755808.1"
                     /translation="MVSLARSLMGGQVAVHVVPGDAFDAHGHFAGPHAAADPDAVLDR
                     IARWADRLTRFTATSELSRLNANARVEVAVGPTLAAVLDWGREAEVLTDGIVDVGLLD
                     ERIAAEGRPETAAHRAGSTPSRSLASRRWSLDRRARGAVVRRPAGLRFDLDGIAKGWI
                     ADRALTSLDGHAAAVVDADGDIAIRLGRGVEWTFGVADPHHTGHDLVELRLRRREGVT
                     DRDEPSSRRAADRFGLATSGTSVHRWDRAGGHAHHLIDPRTGRSAVTDLVQATVLAGS
                     ARVAEALAKAAVIVGSEAAFALLDRAGVDGAILLTDRGRLLIHPATLRWLA"
     gene            1745..2506
                     /locus_tag="VHM48_10115"
     CDS             1745..2506
                     /locus_tag="VHM48_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755809.1"
                     /translation="MTFRGLDARSRWLRWGLLAIGIGIVVGVAGPRAIAALATTWETK
                     RAMLPWVFERLFAFLAYLAMTGSVVYGLLLSTKILDAIAHRPITFALHQDLASIGVGF
                     AAVHGMLLALDKSVPFTLAQIVVPGLAPYAPVWVAAGQLSLFLMAVVIASFYLRRRIG
                     QRAWRALHYVTFLAFVGATAHGIMAGTDSGSSWAWGLYAGSSIAVVFLLVYRIALSAS
                     RRRSRPDGAARRVTAAPEVGRAPLVRAATEASLEA"
     gene            complement(2543..4876)
                     /locus_tag="VHM48_10120"
     CDS             complement(2543..4876)
                     /locus_tag="VHM48_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2755810.1"
                     /translation="MTTPIQPIEMIAFPIEGMTCASCVNRITRFLRKVDGIEDASVNL
                     ATESATVRYDGARVDVAAIVAAVEAAGYVARVERVGTDAADVAAEPATDEPSFADRHR
                     ADLSRRLTVAAVLSTPLLLGLAKMTIAPWLPEFLANPWFQLAFAAPVQVYAGGPFYRG
                     AWNALRHRSADMNTLVAVGTSAAFGYSLAATLVPDFFVAVGLGMDGGQPPLYYDTSAA
                     IITLILLGRFLEARARSHTSDAIRRLIGLAPRTARVVRAGVEADIPIEQVIPGDIVLV
                     RAAERIPVDGTVAAGRSSVDESMITGESMPVGKKLGDEVIGGTVNGAGSFTYQATRVG
                     ADTVLARIVRLVQEAQGSRAPIQRLADAVTGVFVPVVLGIAALTFLAWLAFGPQPAFN
                     YALVSAITVLIIACPCALGLATPTSIMVGTGKGAEHGVLFRNAESLERLQHVRTVVLD
                     KTGTLTEGRPRVTDIVRADGAPEETVLLALVAAAERGSEHPLGEAIVRHVRDAGIEIR
                     ETTAFEAVAGQGVRAAVDGREVLVGRREFVGSADPFLVAAAERAASMGRTPVFVAVDG
                     RPAAVLAVADTLKAGSAAAVAELRRLGLTVVMLTGDNATTAGAIAAQAGVDRVVADVR
                     PEDKAAQVRALQAAGQVVAMVGDGVNDAPALASADVGIAIGTGTDIAIESATVTLMSG
                     DLRGLVTAIALSRATMRNIKENLFWAFAYNVVLIPVAMGVLYPFTGTLLNPILAAGAM
                     AFSSVTVVSNALRLRRFAPPVSGVEPRRTAVPTPALP"
     gene            complement(4932..>6150)
                     /locus_tag="VHM48_10125"
     CDS             complement(4932..>6150)
                     /locus_tag="VHM48_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2755811.1"
                     /translation="AAGYEVGRTSWLERDAQVWATAAGGLLVLVLIAVLAQATGIAGL
                     ASGAGTLSNGGVVVALLLGLAAGVSTCMALVGGVVLALSAANETRRSATAGDVGIVER
                     LRPGLVFVGGRIAGYAVLGAALGALGSSISLPTRFVAVLMIAVAIVMTLIGIRLTGLS
                     PRVAAWSPTLPAGLAGRLGLGSGSVTGYSDTRAALLGAASFFLPCGFTQAVQIYALST
                     GSPVIAGVIMAVFAIGTAPGLLALAGLPAIMPDRARPTLLRVIGVVVIGFAFVNAGAG
                     LRLAGFALPSVGPSVALAAALPPVANSADGRQTLYTFQDAGGYRPGNVAIHAGTPTRW
                     VITSLDSQSCATFLRIPGLDMAVTLHTGRNVIDLPAMPAGSLSYACAMGMYGGTITIL
                     DPPAGAVGATNGG"
BASE COUNT          875 a         2199 c         2270 g          806 t
ORIGIN      
        1 gtcctgaccg cggcgcttgc cgcggccggc ttcgcgcccg gcgcctcacc ccagcccgtc
       61 gcggccgccg actccgtcgc cgcgacgaca ccagaaccga ccgtccaggt cgacaccgtg
      121 tatgtcgccg ctccggcgaa gcagcagacg atcacggtcc acaaggtcgt caagtcgacc
      181 ggcggcgagg agtccgaagg cgagtcgggg ggcgacaatt agatgaacga tctccgaaat
      241 gacgatcgcc cggagccgcg cgatgggtct ggggcgcccg tgagccggcc gccgatgagc
      301 cggccgcttc cgccgcgacc gaccggcccg cggcccgtcg ccgcggcgac gcccgagctc
      361 ccgcctcgcc gggcagtccc ggccgtcgag cgcaaggcgc gacccgatcc gggaccgctc
      421 cgcctcgcct tcgggctcac cgggatggca gccgcgaccg cgctgctcag cgcgttcctc
      481 gtcccggcga ccagcgcgga cgcgggtgcc ggaacgacga acctcgtcac cgtcgccgac
      541 gggccgacac catccgtcca gcacgtgacc cgctatgtcc agctccagcc ggggcagacc
      601 gccccgccgc acgccgtcgt caaccaggcc ccgacgccga aaccgcggat cgtcgtcgtc
      661 acgacccggc aatccggagc caagccatga tcgacgcgat cgcacgtccc gaccgagccg
      721 cctcgcagac ggcgcggatc tcggctccgt ccgagcctcc ggcgatggtc tctctcgcgc
      781 gctcgctcat gggcgggcag gtcgccgttc acgtcgtccc gggcgacgcg ttcgacgcgc
      841 acggccattt cgcagggccc cacgccgccg ccgaccccga tgccgtcctc gaccggatcg
      901 cccggtgggc cgatcgcctg acgcggttca cggcgacctc cgagctctcg cgcctcaacg
      961 ccaacgcacg ggtggaggtc gcggtcgggc cgacgctcgc ggccgtcctg gattggggtc
     1021 gggaggccga ggtcctgacc gacgggatcg tcgacgtcgg attgctcgac gagcggatcg
     1081 ccgcggaagg acggccggag acggccgctc accgtgccgg ctcgacgccg tcgaggtcgc
     1141 tcgcgtcacg tcgatggtcg ctcgatcgac gagcgcgcgg cgccgtcgtc cgccggccgg
     1201 ccgggctgcg gttcgatctc gacggcatcg ccaagggctg gatcgccgat cgggccctca
     1261 cctcgctcga cggccacgcc gcggccgtcg tcgatgccga cggggacatc gccatccggc
     1321 tcggtcgcgg cgtcgagtgg acgttcggcg tcgccgaccc gcaccacacc ggacacgatc
     1381 tcgtcgagct gcgattgcgc cgccgcgaag gtgtcaccga tcgcgacgag cccagcagcc
     1441 ggcgggcggc cgaccggttc ggccttgcca cgtcgggcac gagcgtccat cgctgggacc
     1501 gcgcgggggg ccatgcccat cacctgatcg accccaggac gggacggtcg gccgtgaccg
     1561 atctcgtcca ggcgaccgtc ctcgccggat ccgcacgggt ggcagaggcg cttgccaagg
     1621 cggcggtgat cgtcggctcg gaggcggcgt tcgcgctgct ggatcgggcc ggggtcgacg
     1681 gcgccatcct gctgaccgat cggggccggc tgctcattca tcccgcgacc ctgcggtggc
     1741 tggcatgacg ttccggggtc tcgatgcgcg cagtcgatgg ctccgctggg gcctcctcgc
     1801 gatcggcatc gggatcgtcg tcggggtagc cgggccgcgg gcgatcgcag ccctcgcgac
     1861 gacctgggag acgaagcggg ccatgctgcc gtgggtcttc gagcgtctct tcgcgttcct
     1921 ggcgtacctg gcgatgaccg ggtcggtcgt ctacggactc ctgctgtcga ccaagatcct
     1981 cgatgcgatc gcccatcgcc cgatcacctt cgcgctccat caggacctcg ccagcatcgg
     2041 cgtcgggttc gcggccgtcc acggcatgct gctggccctc gacaagagcg tgccgttcac
     2101 gctcgcccag atcgtcgtgc cgggcctggc tccgtacgcg ccggtctggg tggcggccgg
     2161 gcagctgtcc ctgttcctca tggccgtggt catcgcgagc ttctacctgc ggcgccggat
     2221 cggccagcgc gcctggcggg cgctccacta cgtcacgttc ctcgccttcg tgggcgccac
     2281 cgctcacggc atcatggccg ggaccgacag cggatcgagc tgggcgtggg ggctgtacgc
     2341 cggatcctcg atcgcggtcg tcttccttct cgtctaccgg atcgcgctgt cggcgagccg
     2401 acgacggtcc aggcccgacg gcgccgcgcg tcgcgtgaca gccgcccccg aggtcggccg
     2461 ggcgcccctg gtccgggctg ccacagaggc ctcgctggaa gcctgactcg ttcgcgaccg
     2521 gccgagctgc ggcaggcgcg gtctacggaa gggccggggt cgggaccgct gtccgccgcg
     2581 gctcgacccc ggagaccggc ggggcgaacc ggcgcagccg gagcgcattc gagaccacgg
     2641 tgaccgagct gaacgccatc gctccggccg cgaggatcgg gttcaggagc gtgcccgtga
     2701 atgggtacag cacgcccatc gccaccggaa tgagcacgac gttgtaggcg aacgcccaga
     2761 agaggttctc cttgatgttg cgcatcgtgg cccgcgatag ggcgatcgcg gtcacgaggc
     2821 cgcgcaggtc gccgctcatg agcgtgaccg tggcggactc gatcgcgatg tcggtgccgg
     2881 tgccgatggc gatcccgacg tcggccgagg cgagggccgg agcgtcgttg accccgtcgc
     2941 cgaccatcgc gaccacctgg cccgcggcct ggagcgcccg gacctgggcc gccttgtcct
     3001 cgggtcggac gtcggcgacg acgcggtcga cgcccgcctg ggcggcgatc gcgccggccg
     3061 tcgtcgcgtt gtcaccggtc agcatgacga ccgtcagccc gagccggcgg agctcggcga
     3121 ccgcggctgc ggatccggcc ttcagcgtgt cggcaaccgc gaggacggcg gcgggccggc
     3181 cgtcgacagc cacgaagacc ggcgtccggc ccatcgacgc cgctcgctcg gcggccgcga
     3241 cgaggaatgg atcggcagac ccgacgaatt cgcgccgacc gacgagcacc tcgcgcccgt
     3301 cgacggcggc gcgcacgccc tggccggcca cggcttcgaa ggccgtggtc tcgcgaatct
     3361 cgatcccggc atcgcgaacg tggcggacga tcgcctcgcc cagcggatgc tccgagccac
     3421 gctcggctgc ggcgacgagc gcgagaagga ccgtctcctc gggtgcgcca tcggcccgga
     3481 cgatgtcggt cacgcgcggc cggccctcgg tgagcgtgcc ggtcttgtcg agcacgaccg
     3541 tccggacgtg ctggagccgc tcaagcgatt ccgcgttccg gaacaggacc ccgtgttcgg
     3601 cgcccttgcc ggtcccgacc atgatcgagg tgggggtggc gagcccgagg gcgcagggac
     3661 aggcgatgat caggacggtg atcgcactga cgagcgcgta attgaacgcc ggctggggcc
     3721 cgaaggcgag ccaggcgagg aaggtcaggg ccgcgatccc gaggacgacg gggacgaaca
     3781 cgccggtcac cgcatccgcg agccgctgga tcggtgcccg cgagccctgc gcctcctgga
     3841 cgaggcgcac gatccgggcg aggaccgtgt cggctccgac gcgggtcgcc tggtaggtga
     3901 acgagccggc gccgttgacg gtgccgccga tgacctcgtc gccgagcttc ttgccgacgg
     3961 gcatgctctc gccggtgatc atgctctcgt cgaccgacga ccggcccgct gcgacggtcc
     4021 cgtcgaccgg gatccgctcg gcggcccgga cgagcacgat gtcacccggg atgacctgct
     4081 cgatcgggat gtctgcctcg acgccggcac ggacgacgcg cgccgtgcgc ggtgcgaggc
     4141 cgatgagcct gcggatcgcg tcggaggtat ggctgcgggc gcgagcctcg aggaagcggc
     4201 cgagcaggat cagagtgatg atcgcggccg aggtgtcgta gtagagcggg ggctgcccgc
     4261 cgtccatccc gaggccgacc gcgacaaaga agtccgggac cagggtcgcc gcgaggctgt
     4321 acccgaacgc ggcgctggtg ccgaccgcga ccagcgtgtt catgtcggcg gagcggtggc
     4381 gcagcgcatt ccacgcgccc cgatagaagg ggccgccggc gtagacctgg accggcgcgg
     4441 cgaaggcgag ctggaaccat gggttcgcca ggaactccgg cagccagggc gcgatcgtca
     4501 tcttcgcaag cccgaggagc aggggggtgc tgaggactgc cgccacggta agccgccggc
     4561 tgaggtctgc acgatgccgg tcggcgaacg acggctcgtc ggtcgcgggc tcggccgcga
     4621 cgtcggcggc gtcggtgccg acgcgttcga cgcgcgcgac gtagccggcc gcctcgacgg
     4681 cggcgacgat cgccgcgacg tcgacccgtg ccccgtcgta gcgcacggtc gccgactcgg
     4741 tcgcgagatt cacgctcgcg tcctcgatcc cgtcgacctt gcgcaggaag cgcgtgatcc
     4801 ggttgacgca cgacgcgcag gtcatgccct cgatcgggaa ggcgatcatc tcgatcggct
     4861 ggatgggcgt cgtcacgaca ggctcctgtg ggttcgagtt gcgttcggcg gatgacaggt
     4921 tggtggtcac gtcagccgcc attcgtggcg ccgaccgcgc ccgccggcgg gtcgaggatc
     4981 gtgatcgtcc cgccgtacat ccccatcgcg caggcgtagg acagggaccc cgcgggcatt
     5041 gccgggagat cgatcacgtt gcgtcccgtg tggagggtca cggccatgtc gagcccgggg
     5101 atccgcagga acgtggcgca ggactgggag tcgagcgacg tgatcaccca gcgcgtcggg
     5161 gtgccggcgt ggatggcgac gttgcccggg cggtacccgc cggcgtcctg gaaggtgtag
     5221 agggtctgcc tgccgtccgc ggaattggcg acggggggca gggccgcagc gagggcgacg
     5281 ctcgggccga ccgacgggag cgcgaacccg gcgagccgca gcccggcgcc ggcattgacg
     5341 aacgcgaagc cgatgacgac gaccccgatg acgcgcagca gggtcgggcg ggcacggtcc
     5401 ggcatgatcg cgggcaatcc ggcgagcgcg aggaggccgg gcgcggtccc gatcgcgaag
     5461 acggccatga tcacgccggc gatgaccggc gatccggtcg agagggcgta gatctggacg
     5521 gcctgggtga agccgcatgg caggaagaac gaggccgcac cgaggagcgc agcgcgggtg
     5581 tcggaatatc cggtgacgga gccgctgccg agcccgagcc tgcccgcaag accggccggg
     5641 agcgtcgggg accaggccgc gacccgcggc gaaagcccgg tcaggcggat cccgatcagg
     5701 gtcatgacga tcgcgaccgc gatcatgagg acggcgacga accgggtcgg gagggagatg
     5761 ctcgaaccga gcgcgccgag agccgccccg agcaccgcgt aaccggcgat ccggcccccg
     5821 acgaacacga ggcccggtcg caggcgctcg acgatcccca cgtcgccggc cgtcgcggac
     5881 cgccgcgtct cgttcgccgc ggagagggcg aggacgacgc cgccgacgag ggccatgcac
     5941 gtcgataccc cggcggcgag cccgagcagg agcgcgacga cgacgccgcc gttgctcagg
     6001 gttcccgccc cggacgcgag cccggcgatc cccgtcgcct gggcgaggac cgcgatcagg
     6061 acgaggacga ggagcccgcc ggcggcggtc gcccagacct gggcgtcgcg ctcgagccat
     6121 gacgtccggc ccacctcgta ccccgcggcc
//
LOCUS       DATAIW010000330         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_247501, whole genome shotgun sequence.
ACCESSION   DATAIW010000330 DATAIW010000000
VERSION     DATAIW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247501"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..684
                     /locus_tag="VHM48_10130"
     CDS             226..684
                     /locus_tag="VHM48_10130"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2755812.1"
                     /translation="MPSWFPILLVTHIVLAVGLFLPSILLPFALRTRRAAADSPHVFV
                     RFLLWMQAHGTLVIGLGLAVTGTALVLLLGASLLRQPWLILALSIYAANLLLAFLVQR
                     PNLRRLVGIRAATDDRLWLDRAKRQRYVSYAMAALVGTIGFLMSTKPQLW"
     gene            695..2137
                     /gene="mtaB"
                     /locus_tag="VHM48_10135"
     CDS             695..2137
                     /gene="mtaB"
                     /locus_tag="VHM48_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX2755813.1"
                     /translation="MGDAASTRPIRTAAITNLGCKVNQSEMEGAARRLRERGIAIVDG
                     DRPADLVLVNTCTVTAEADAKSRHAVRRARRVSPDAAVVVTGCSVQVGREAFAAADPD
                     ARLVDNRSKDALLEELDVLLGPGGPGGPGRRGGLVDRALPTLSGVAIEGIADERASVE
                     RTRAFVKVQDGCSFFCTYCIIPSARGPERSLAPEVVMADVRRALGAGHREIVLTGINI
                     GTYDGGWSERGFRGAHRRSELTLAGLVRRLLDETPVERIRLSSIEPQHVDDALLRAWV
                     DGAPRTMPHVHLPLQSGDDGVLRRMGRRYTGAGYAAMVARVRDAIPGVAVHGDVIVGF
                     PTEEAAAWARSLAFIRSIEFAGIHVFRYSGRPGTPATRMTGQVDEATKKARAGALLAV
                     AADARERWAGGRVGGVGDVLFESRLDDGRWVGHAADHTSVAVRPTDGASLENALGRVR
                     LDAVDPDRRDRLVGRILSMSPRRGVPVHAS"
     gene            2127..2492
                     /locus_tag="VHM48_10140"
     CDS             2127..2492
                     /locus_tag="VHM48_10140"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX2755814.1"
                     /translation="MPADRPSEPDCLFCRIVRGEVPAKTVVDDDLVLAFHDIAPRSPT
                     HVLVIPKTHIPSAADLTEDDGPLLGRLFATAARIARDAGIASDGYRIVSNVGRWGGQT
                     VDHFHLHLMGGRAFGWPPG"
     gene            2522..3049
                     /locus_tag="VHM48_10145"
     CDS             2522..3049
                     /locus_tag="VHM48_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755815.1"
                     /translation="MLALVAALGLAGCGIGANGSPASFAPASVGPATNVTGATAQTRG
                     EIAVALASISSVAIQFGDATRPYRPPESGRLRDAPRAVYQVVMPNLPDAGFVVVYEFA
                     DATAASDAGNEEAGFLGTGPARVNFPRDAQHVLRQVGPTLILYTWSPSESTDRTASTV
                     AAVLATLGIGFSVPN"
     gene            complement(3033..>3914)
                     /locus_tag="VHM48_10150"
     CDS             complement(3033..>3914)
                     /locus_tag="VHM48_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2755816.1"
                     /translation="DRSGQRARFDRLRGIARHLPYPVRRVLFRRVHRRLGGGMRLFVS
                     SGAFLPPALQQAWEDLGIVVVQGYGSSENGFGTCTTPQDHGLGTVGRAMPPVELRVAD
                     DGEILFRGPTLFKGYWHDPETTARAIDADGWYHTGDIGHLDADGRLIMSGRTKDRIVL
                     PNGFKVYPEDLENALRVAGLRDAIVVESAPGRIEAIVLRGAADEELADSGRRTASTDD
                     AERAVVDSSVKRANAALGPQQRIVGWRFWPDDDFPRTHTLKIRRDPIRAWAAGEGPQD
                     AASAIPQPDGTDVSSGR"
BASE COUNT          485 a         1426 c         1402 g          601 t
ORIGIN      
        1 gctccgtccg ggcggcgtgc tactcgcgtc ggggatcttc gtcgaccggg agagcgaggt
       61 cggcgctgcg ttcgaggcgg tcgggctggc cgtcacggtg cggacggccg agggcgaatg
      121 ggtcgcactc gaggcaacgc ggcccgtgta ggctgcgcga tcgaaggtcc gatccggctg
      181 gtccggatcc tggtcgatcg ctgacccgcc ctacaatcgg gcggcatgcc cagctggttc
      241 ccgatcctgc tcgtcaccca catcgtgctc gcggtgggtc tcttcctgcc gtcgatcctc
      301 ttgccgttcg ccctccggac gcggcgggcc gctgccgaca gcccgcatgt cttcgtccgg
      361 ttcctgctct ggatgcaggc ccacgggacg ctggtgatcg ggctcggcct ggccgtcacg
      421 gggaccgcgc tggtcctcct gctcggggcg tcgctgctcc gccagccgtg gctgatcctc
      481 gccctgtcga tctatgccgc gaacctgctc ctcgcgttcc tcgtccagcg cccgaacctg
      541 cgccggctcg tcgggatccg ggccgcgacg gacgaccgcc tctggctcga tcgggcgaag
      601 cggcagcgct acgtcagcta cgcgatggcg gccctcgtcg gaacgatcgg attcctgatg
      661 agcacgaagc cgcagctgtg gtgaggtcct gacgatgggc gacgcggcgt ccacccgccc
      721 gatccggacg gcggccatca cgaatctcgg ctgcaaggtc aaccagtccg agatggaggg
      781 tgcggcgcgg cgcctgcggg agcgcggaat cgcgatcgtc gacggcgatc ggccggccga
      841 cctcgtcctc gtcaacacct gcaccgtgac ggccgaggcc gacgcgaagt cgcgccatgc
      901 ggtccggcgc gcccggcgcg tcagcccgga cgcggcggtc gtcgtgaccg gctgctcggt
      961 ccaggtcggg cgcgaggcgt tcgccgccgc cgatcccgac gcccgtctgg tcgacaaccg
     1021 ctccaaggac gcgctgctcg aggagctcga tgtgctgctc gggcccggcg gcccgggagg
     1081 gccgggccgc cggggcgggc tggtcgaccg ggccctgccg accctgtcgg gtgtcgcgat
     1141 cgaggggatc gccgacgagc gggcatcggt cgagcggacc cgggcgttcg tcaaggtcca
     1201 ggacggctgc tcgttcttct gcacctactg catcatcccg agcgcccgcg gccccgagcg
     1261 cagcctcgcc ccggaggtgg tcatggccga cgtccgccgg gcgctggggg ccggtcatcg
     1321 cgagatcgtc ctgaccggga tcaacatcgg gacgtacgac ggcggctggt ccgaacgcgg
     1381 gttccggggc gcccaccgcc gctcggagtt gacgctcgcc gggctcgtcc ggcggctcct
     1441 cgacgagacg ccggtggaac ggatccggct gtcctcgatc gagccccagc acgtcgacga
     1501 cgcgctgctc cgggcgtggg tcgatggcgc gccgcggacg atgccccacg tccacctgcc
     1561 gctccagtcg ggcgacgacg gcgtgctccg gcggatgggc cggcgctaca cgggggccgg
     1621 gtatgccgcg atggtcgcgc gcgtccggga cgcgatcccc ggcgtcgcgg tccacggcga
     1681 cgtgatcgtc gggttcccga ccgaggaggc ggcggcgtgg gcgcggtcgc tggcgttcat
     1741 ccgctcgatc gagttcgccg gcatccacgt gttccgctac tccggccgac caggaactcc
     1801 cgcgacccgg atgaccggcc aggtcgacga ggcgacgaag aaggcgcgcg cgggcgcgct
     1861 ccttgcggtg gccgccgacg cgcgcgagcg ctgggcgggc ggtcgagtgg gaggcgtcgg
     1921 cgacgtcctg ttcgagtcac gtctcgacga cggccggtgg gtcggccacg ccgccgacca
     1981 cacgtcggtc gcggttcgtc ccacggacgg tgcctcgctc gagaacgcgc tcggccgcgt
     2041 ccgcctcgat gccgtcgatc cggatcggcg cgatcggctc gtcggccgca tcctgtccat
     2101 gtcaccccgt cgcggagtcc ccgtccatgc cagctgaccg accatccgag cccgattgcc
     2161 tgttctgccg gatcgtccgg ggcgaggtgc cggcgaagac cgtcgtcgac gacgacctcg
     2221 tcctcgcctt ccacgacatc gccccgcgca gcccgaccca cgtcctcgtg atcccgaaga
     2281 cgcacatccc ctcggccgcc gacctgaccg aggacgacgg tccgctcctc gggcggttgt
     2341 tcgccacggc tgcgcgcatc gcccgtgacg ccgggatcgc gtcggacggc taccggatcg
     2401 tcagcaacgt cgggcgctgg ggcggtcaga cggtcgacca cttccatctc cacctgatgg
     2461 gcggtcgggc gttcggttgg ccgccgggat gagaagggcc gccgcttcga ccacgttcgt
     2521 cttgctcgcg ctcgttgctg cgttgggcct ggccggctgc gggatcggtg cgaacgggag
     2581 cccggcctcg ttcgcgcccg cgagcgtcgg ccccgcaacg aacgtcaccg gcgcgaccgc
     2641 ccagactcgt ggcgagatcg ccgtggcgct ggcgtcgatt tcgtcggtgg ccatccagtt
     2701 cggcgacgcg acccggccgt atcgcccacc cgagtcgggg cggctgcgcg acgcgccgcg
     2761 ggccgtctac caggtggtca tgccgaacct gcccgacgcg ggcttcgtcg tcgtctacga
     2821 attcgccgac gcaacagctg cctccgacgc cggcaacgag gaggccggct ttctcgggac
     2881 cggccccgcc agggtgaact tcccacggga tgcccagcac gtcctccgtc aggtcggccc
     2941 gaccctgatc ctgtacacgt ggtcgccgtc cgagtcgacc gaccggaccg catcgaccgt
     3001 cgccgccgtg ctcgcgaccc tgggaatcgg gttcagcgtc ccgaactgac gtcggttcca
     3061 tctggttgcg ggatcgcgga cgcggcgtcc tgcggccctt cgcccgcggc ccaggcccgg
     3121 atcgggtccc gccggatctt caacgtgtgc gtccggggga agtcgtcgtc cggccagaat
     3181 cgccagccga cgatccgctg ctgcggcccg agcgccgcgt tcgcgcgctt caccgacgag
     3241 tccacgaccg ctcgctcggc gtcgtcggtg gaggccgtcc gcctgccgct gtcggcaagc
     3301 tcctcgtcgg cagcgccgcg gaggacgatg gcctcgatcc tgccgggcgc cgattcgacg
     3361 acgatcgcgt cgcgcagccc cgccacgcgc agcgcgttct cgagatcctc cgggtagacc
     3421 ttgaagccgt tcggcaggac gatccgatcc ttcgtccggc cgctcatgat cagtcgtccg
     3481 tcggcgtcga ggtggccgat gtcgccggtg tggtaccagc cgtcggcgtc gatcgcgcgc
     3541 gccgtcgtct cggggtcgtg ccagtagccc ttgaacaggg tcggcccgcg gaagaggatc
     3601 tcgccgtcgt cggcgacccg gagctcgacc ggcggcatcg cccggccgac cgtcccaagc
     3661 ccgtgatcct gcggtgtcgt gcaggttccg aagccgttct cgctgctgcc gtagccttgg
     3721 acaacgacga ttccgaggtc ctcccaggcc tgttgcaggg ccggcggcag gaacgcgccg
     3781 gacgagacga agagacgcat cccgccgccg agccggcggt ggaccctccg gaagagcaca
     3841 cgccggaccg ggtaggggag gtggcgggcg atgccacgca ggcgatcgaa gcgggcccgt
     3901 tgtccggatc gatc
//
LOCUS       DATAIW010000331         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_51031, whole genome shotgun sequence.
ACCESSION   DATAIW010000331 DATAIW010000000
VERSION     DATAIW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51031"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VHM48_10155"
     CDS             <1..619
                     /locus_tag="VHM48_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755817.1"
                     /translation="IWAAPAQPAAGSDPFERPAAGMPVEAAPVRAKGNGGMLVNLLLG
                     VALVIAVGGVAFAVGRATAPASTATGRTGLGANGGGLFQGGPDASGAPGGTFNGGFGV
                     GGGGVSIEGTVTAVSADSITLQLASGQTITIPTDAQTTYHQRTATTAAAVTSGSTIIV
                     QLQGGRGAFGNGNGNGGQGGGAQASGAPGRTLGPASTVTVIPSGG"
     gene            complement(639..1448)
                     /locus_tag="VHM48_10160"
     CDS             complement(639..1448)
                     /locus_tag="VHM48_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755818.1"
                     /translation="MSDLLRLSSGPAVAVVDLEHGGRLASLVIDGCEVLVTTGWGPFS
                     WGSFPMVPFAGRLRDGWFAFGGRTHQLPLNQPPFALHGLVGERPWTRCPEAAGPNAIG
                     CELVAPWPYRGRAIQSFDLADDRLDVSLSLEAGEPQPATIGWHPWFRRRLEPAGSGDP
                     VQLEIDPGRMYRRGPDGLPTGELIAPGPHPWDDCVTDLRRPPIVRWPGALEIELTSTA
                     DRWVVYEASPDGICAEPQTGPPDGLNLAPQVVRPGEPLRIEMTWRWRPNPG"
     gene            complement(1445..2743)
                     /locus_tag="VHM48_10165"
     CDS             complement(1445..2743)
                     /locus_tag="VHM48_10165"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755819.1"
                     /translation="MTPSPRTERSYRALLAVPGLPRILVSMQLSRIAQAMVGVALVLF
                     TLAEYDSPALTGLVTFASVFPGLVVAPIAGALLDRHGRTRLVILDYAVALLALVLIGA
                     LALAHALPVPVLLAIAIVSSLTSILSHTGLRSLFPLIVPERLWERVNAIDSNGYLVAT
                     ILGPPLAAALVTIVGGATTLILIGIVYGGATVAMIGAPDPVSATASTGRLLVDAWDGV
                     KYTLRNPTLRGLGVSISLLNLSGGMVTIVVPLIVIDRLGAPEVVVGLVFAGSGVAGMV
                     AALIAGRFDSRGREWNLLVLPMAGIALADLLLLAGAGAAETLTGLCFVGLGLALGGAL
                     NGPMDVGLFTIRQRRTDPAWMGRAFAVSMALNFVGYPIGAALAGGLAAVSIEAAIIVG
                     AAACLVAGVAAALMIPRHVARTQVAVDERTARDDPALTSR"
     gene            2938..4053
                     /gene="ugpC"
                     /locus_tag="VHM48_10170"
     CDS             2938..4053
                     /gene="ugpC"
                     /locus_tag="VHM48_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2755820.1"
                     /translation="MATVTFEHVTKKYGDVLAVNDLNLAINDGEFMVLVGPSGCGKTT
                     SLRMIAGLEEITNGDLKIGDRVVNDVPPKDRDIAMVFQSYALYPHMTVRDNMAFGLKL
                     RKLPKADIERRVNEAAGTLSLEKLLDRKPKELSGGQRQRVALGRAIVREPAVFLMDEP
                     LSNLDAKLRVQTRAEIARLHQRMGTTFVYVTHDQVEAMTMGTRIAVMSDGLLQQVGTP
                     QVLYDQPTNRFVAGFIGSPSMNFLDVTFDSSGGTPKLAGPGGWTAALPHRFAASVGAT
                     SGRKLVAGFRPEHLELDPVEGDTVHLPGKADVVEYLGNEELLHVTAAEKDIVAIVGSD
                     HRVKPGDMLDLILPLTKLHLFDTESGLSLTSERETAA"
     gene            4146..4763
                     /locus_tag="VHM48_10175"
     CDS             4146..4763
                     /locus_tag="VHM48_10175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2755821.1"
                     /translation="MSIGPTDAELIERVVATRVLHRGRYLEFRVDTIERADGTRSERD
                     IAGHPGAVAIVALDSDDRVLLVRQWRTPAEQVLLEIPAGTLDIDQATGAAEDPDLAAR
                     RELEEETGYRAGRWERLTSFWSAPGFTSELMHLYLATELVAAHPDERLGPDEDEHLRL
                     ERRPLVDALAAVDRGEIADAKSIVGLLWLARRQSADAAGRRRHGT"
     gene            4778..5677
                     /locus_tag="VHM48_10180"
     CDS             4778..5677
                     /locus_tag="VHM48_10180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755822.1"
                     /translation="MTTMGVRTFRRGDEPGIIAVIDAGLSVDRLPGITRQDLLHGVER
                     MAGDPEGTLVATENGAIVGFCTPRMDELTIHPAHRRRGHGRRLVTEWLTRRRAAGEPD
                     LVLHGPDRPAAAGFIAALGFVRRSSLWLFELAPDAAVPAPAFPADVRVRTYRDGDLAR
                     FVEVANASFADHPSPVLFTERGVGHVHGLSDFDPDAILLVFASDDPDRAIGWAKAEYE
                     LVDGTAERRGFVSFVGIVPAWRGRGLGRALLRWAIERCRAAGARTIELSVEAANDRAL
                     GLYRSTGFTAEVEWPHYALATGA"
BASE COUNT          898 a         2100 c         2103 g          761 t
ORIGIN      
        1 gatctgggcg gcgccggccc agccggccgc gggctccgat cccttcgagc ggcccgccgc
       61 gggcatgccc gtcgaggcgg ccccggtccg cgccaagggc aacgggggaa tgctcgtgaa
      121 cctccttctc ggcgtcgccc tggtgatcgc ggtcggcggc gtcgccttcg cggtcggccg
      181 agccaccgcg ccggcctcga ccgcgaccgg tcggaccggc ctcggggcca acggcggcgg
      241 gctcttccag ggcggaccgg atgcgagcgg cgcgccgggc ggcacgttca acggcgggtt
      301 cggcgtgggt ggaggcggcg tctcgatcga gggcaccgtc accgccgtga gcgccgattc
      361 gatcacgctc cagctcgcga gcgggcagac gatcacgatc ccgaccgacg cccagacgac
      421 gtatcaccag cggacggcga cgaccgcggc cgccgtgacc tccggcagca cgatcatcgt
      481 ccagctccag ggcgggcggg gcgcgttcgg caacggcaac ggcaacggtg gccagggcgg
      541 tggcgcgcag gcgagcggcg ccccgggccg gacgctcggg ccggcgagca cggtgaccgt
      601 gattccgtcg ggcggctgag ccgacccggc cgtcgtcgtc agcctgggtt cggccgccaa
      661 cgccaggtca tctcgatccg caggggctcg cccggccgga cgacctgcgg cgcaaggttc
      721 agtccgtcgg gcggcccggt ctgcggctcg gcgcagatcc cgtccggcga cgcctcgtag
      781 acgacccatc gatcggcggt cgaggtcaac tcgatttcga gcgcgcccgg ccagcggacg
      841 atcggcggcc ggcgcaggtc cgtcacgcag tcatcccacg gatgcgggcc gggcgcgatg
      901 agctcgccgg tcgggaggcc gtcagggccc cggcggtaca tccgcccggg atcgatctcg
      961 agctggaccg ggtctccgga gccggccggc tcgagccgac ggcggaacca cggatgccag
     1021 ccgatcgttg ccggctgtgg ttcgcccgcc tcgaggctca acgacacatc gagccgatcg
     1081 tccgcaaggt cgaacgactg gatcgcccgg ccgcggtacg gccagggcgc caccagttcg
     1141 cagccgatcg cgttcggacc cgctgcctcg ggacagcgcg tccagggtcg ctcgccgacc
     1201 aggccgtgaa gcgcgaacgg cggctggttg aggggcagct gatgcgtccg gccaccgaag
     1261 gcgaaccacc cgtcccgcag acgcccggcg aacggaacca tcgggaacga accccaggag
     1321 aacggacccc agccggtcgt gacgaggacc tcgcaaccgt cgatgacgag cgaggcaagg
     1381 cggccgccgt gctcgaggtc gacgacggca accgcggggc ccgaagacag gcggagcagg
     1441 tcgctcatcg cgaggtcaac gccggatcat cccgggcggt ccgctcgtca accgcgacct
     1501 gcgttctcgc gacgtggcgc gggatcatca acgcggccgc cacgccggcg acgaggcagg
     1561 ccgccgcgcc gacgatgatc gcggcctcga tggagactgc cgcaagaccg ccggcgaggg
     1621 cggctccgat cggatagccg acgaagttca aggccattga tacggcgaac gcgcgaccca
     1681 tccatgcggg atcggtccgg cgctggcgaa tcgtgaacag cccgacgtcc atcggcccgt
     1741 tgagcgctcc cccgagcgcc agccccagcc cgacgaagca caggccggtc aaggtctcgg
     1801 ctgcccccgc cccggccagg aggaggaggt cggcgagcgc gatcccggcc atcggcagga
     1861 ccagcaggtt ccattcccgg ccgcggctgt cgaagcgacc ggcgatcagg gcggccacca
     1921 tgcccgcgac ccctgacccg gcgaacacca gcccaaccac gacctccggg gcgcccaacc
     1981 ggtcgatgac gatcagcggg acgacgatcg tgaccatgcc gcccgacagg ttgagcaggg
     2041 agatcgagac cccgaggccg cgcaaggtcg ggtttcggag cgtgtacttg acgccgtccc
     2101 acgcgtcgac cagcaggcgt ccggttgacg ccgtcgccga caccgggtcc ggggcgccga
     2161 tcatcgcgac cgtcgccccg ccgtacacga tcccgatgag gatcagggtc gtcgccccgc
     2221 cgacgatggt gacgagggcc gcggccagcg gcggtccgag gatcgtcgca acgaggtaac
     2281 cgttcgagtc gatcgcgttg acccgttccc acagacgctc cgggacgatg agcgggaaga
     2341 ggctgcggag ccccgtgtgg gacaggatcg aggtcaggga gctgacgatc gcgatcgcga
     2401 ggaggaccgg gaccggcagt gcgtgggcca gggccagagc gccgatcagg acgagggcca
     2461 gcaaggcgac ggcgtagtcg aggatgacca ggcgcgtccg accgtgacga tcgaggaggg
     2521 cgccggcgat cggcgccacg acgagcccgg gaaagacgct ggcgaacgtc acgagtccgg
     2581 tcagcgcggg cgagtcgtac tcggcgagcg tgaacagcac gagcgccacc ccgaccatcg
     2641 cctgggcgat ccggctgagc tgcatcgaga ccaggatccg gggcaggccg ggaaccgcga
     2701 gcagggcgcg gtaggagcgt tcggtccgtg ggctcggagt cacctgccgg atgctatcgg
     2761 tggttggcgt cgggcgctgg tgatcgcgtt ctgcaacgga tcgtgtgcct cgacaagggt
     2821 ccgcccggtg gtacgatccg ggcactcgag ccggctcctc gtacggggtc cgtccacgac
     2881 gccgaatggc ggctggatgc agggacccgc gatgccccgg tggagtgagg tgcacgcatg
     2941 gccaccgtga cattcgagca cgtcaccaag aagtatggtg acgtcctcgc tgtcaacgac
     3001 ctgaacctcg ccatcaacga cggcgagttc atggtcctcg tcgggccctc cgggtgcggc
     3061 aagacgacga gcctccggat gatcgccggc ctggaggaga tcacgaacgg ggacctcaag
     3121 atcggcgacc gggtcgtcaa cgatgtcccg cccaaggacc gcgatatcgc gatggtcttc
     3181 cagagctatg cgctctatcc ccacatgacc gtccgcgaca acatggcgtt cgggctgaag
     3241 ctgcgcaagc tgcccaaggc cgacatcgag cgccgggtca acgaggcggc tgggacgctg
     3301 agcctcgaga agctcctcga tcgcaagccg aaggagctgt ccggcggcca gcgtcagcgc
     3361 gtcgcgctcg gtcgggcgat cgtccgcgaa ccggccgtgt tcctcatgga cgagccgctc
     3421 tccaacctcg acgccaagct gcgcgtccag acccgggccg agatcgcccg cctccaccag
     3481 cggatgggga cgacgttcgt gtatgtcacc cacgaccagg tcgaggcaat gacgatgggc
     3541 acccggatcg cggtcatgag cgacggcctg cttcagcagg tcgggacgcc gcaggtcctc
     3601 tacgaccagc cgacgaaccg cttcgtggcc ggcttcatcg gcagcccgtc gatgaacttc
     3661 ctcgacgtga cgttcgactc gtcgggcggg acgccgaagc tcgccggacc gggcggctgg
     3721 accgccgccc tgccccatcg cttcgccgcc tcggtcgggg ccacgtcggg ccgcaagctc
     3781 gtggccggct tccggccgga gcacctcgag ctcgacccgg tcgagggcga cacggtccat
     3841 ctgccgggca aggcggacgt cgtcgagtat ctcgggaacg aggagctgct ccacgtgacc
     3901 gcggccgaga aggacatcgt cgcgatcgtc ggctccgacc atcgggtcaa gccgggcgac
     3961 atgctcgacc tcatcctgcc gctcacgaag ctccacctgt tcgacaccga gtccggcctc
     4021 agcctgacgt ccgagcgcga gacggcggcc tgaccgagag aaccgatcgg accggccccg
     4081 ccggtccgat caccagtccg acgacgggcg cggcgccgga tccggcaacc gcgcccgaag
     4141 accccatgag catcgggccg accgatgccg agctcatcga gcgcgtcgtc gccacgcgcg
     4201 tgctccatcg cggccgttac ctcgagttcc gggtcgacac gatcgagcgg gccgacggca
     4261 ctcgctccga gcgcgacatc gccggccacc cgggtgcggt ggccattgtc gcgctcgatt
     4321 ccgacgaccg cgtcctgctc gttcgccagt ggcggacgcc agccgagcag gtcctgctcg
     4381 agatcccggc gggcaccctc gacatcgacc aggcgacggg cgccgccgag gaccccgacc
     4441 tcgcggcccg gcgcgagctc gaggaggaga ccggctaccg cgccgggcgc tgggagcggc
     4501 tgacctcgtt ctggtcggcg cccgggttca cgagcgagct gatgcacctg tacctcgcaa
     4561 ccgagctcgt cgccgcccat cccgacgaac gcctcgggcc cgacgaggac gagcatctcc
     4621 gcctcgagcg gcgcccgctc gtcgatgccc tggccgcggt cgatcggggc gagatcgccg
     4681 acgcgaagtc gatcgtcggc ctcctctggc tggcccgccg gcaatcggcg gacgcggccg
     4741 gacgccggcg ccacgggacc tgagccgctc cccggcgatg acgaccatgg gagtccgcac
     4801 cttccggcgg ggcgacgagc ccgggatcat cgccgtgatc gacgcggggc tgtcggtgga
     4861 ccggttgccg gggatcaccc gccaggacct cctccacggc gtcgagcgga tggcgggcga
     4921 tcccgagggc accctggtcg cgaccgagaa cggcgcgatc gtcggcttct gcacgccgcg
     4981 gatggacgag ctgacgatcc acccggcgca tcgtcgccgc gggcacggcc ggcggctggt
     5041 cacggagtgg ctgacgcggc gcagggcggc cggcgagccc gacctcgtcc tccatggccc
     5101 ggatcgtccg gccgccgccg ggttcatcgc cgcgctcggg ttcgtccgcc gctccagcct
     5161 ctggctgttc gagctcgcgc ccgatgctgc agtcccggcg ccggcctttc ccgccgacgt
     5221 ccgcgttcgg acctatcgcg acggcgacct ggcccggttc gtcgaggtcg cgaacgcctc
     5281 gttcgcggat catccgtcgc cggtcctgtt caccgaacgg ggggtcggcc atgtccacgg
     5341 actgtccgac ttcgaccccg acgcgatcct cctcgtcttc gcctccgacg atccggaccg
     5401 cgcgatcggc tgggccaagg ccgaatacga gctcgtggac gggaccgccg agcgacgcgg
     5461 gttcgtctcg ttcgtcggca tcgttccggc ctggcgcggg cgaggtctcg gacgcgcgct
     5521 gctccgctgg gcgatcgagc gttgccgcgc ggccggggcg cggacgatcg agctgagcgt
     5581 cgaggcggcc aacgaccggg cgctcggcct ctatcgctcg acgggcttca cggccgaggt
     5641 cgaatggcct cactacgcgc tcgcgaccgg cgcctgaccg ggatcgcctc gcgccgatcg
     5701 gcggtcggct actcgttgcc caggcgggcg agcgccccgg cgtagacctt cgaggtcggg
     5761 ctgagggcga ccgccacgcg aaggtgcgtt cgcgcttcgt cacgccgacc caactgcttg
     5821 aggctctggc ccagggcgaa atgggcgtaa tgggccgacg ga
//
LOCUS       DATAIW010000332         9737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_187986, whole genome shotgun sequence.
ACCESSION   DATAIW010000332 DATAIW010000000
VERSION     DATAIW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187986"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHM48_10185"
     CDS             <1..582
                     /locus_tag="VHM48_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755823.1"
                     /translation="RDEQRLFTALDGLLGQVTEEQAETALNILAPGSDGASRYREGFP
                     AAGAEELYELVNSDWGFRMPTLHLAEAQIAAGGRAHVYELTWAAPGMGGILGACHGLD
                     VPLVFGNADRGQPAMLIGDSPNPEAEMLSARMRAAWTAFATHGDPGWIAYDTQRRLVQ
                     LFDTRPTIAADPEETSRLIWQDHAFPALPLIGR"
     gene            complement(652..727)
                     /locus_tag="VHM48_10190"
     tRNA            complement(652..727)
                     /locus_tag="VHM48_10190"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:Val,seq:cac)
     gene            complement(772..1053)
                     /locus_tag="VHM48_10195"
     CDS             complement(772..1053)
                     /locus_tag="VHM48_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEX2755824.1"
                     /translation="MADIDVRFAVRLTPRSGSDHVDGVVDGVLRAHVAAPAVGGAANQ
                     SLLRLLAEELGVPRRSVRLVAGAAGRQKLVVIDGLEPAALIARYPGLRV"
     gene            complement(1057..1299)
                     /locus_tag="VHM48_10200"
     CDS             complement(1057..1299)
                     /locus_tag="VHM48_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2755825.1"
                     /translation="MGVLVANLLQIAFLAVWLLVLGRVFLSWFDPAGRSPVGSFVISA
                     TEPILAPIRRVLPPTGALDLSPLVVLVVLSILWQVV"
     gene            complement(1359..2087)
                     /locus_tag="VHM48_10205"
     CDS             complement(1359..2087)
                     /locus_tag="VHM48_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX2755826.1"
                     /translation="MVAPATADLSGFAAARERVLANIADACARVGRDPADITLVAVSK
                     TVPAERLVAAVAVGLTTLGENRVQEAETKVGVVDGATWHMIGPLQSNKARRAVAAFAV
                     IESVDSVELAERLDRMANEVRPGERLPVLLQVNVDADAAKSGFDPDGLAAALPRLADL
                     EHLELRGLMTVGRLVERAEDARSTFVALRELSVRLRQLDRGGSPALGPALSMGMSDDY
                     QVAIEEGATIVRVGRALFGERHHP"
     gene            complement(2100..3329)
                     /gene="ftsZ"
                     /locus_tag="VHM48_10210"
     CDS             complement(2100..3329)
                     /gene="ftsZ"
                     /locus_tag="VHM48_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2755827.1"
                     /translation="MALRSDSENFALIRVIGVGGGGSNAVNRMIRAEMMGVEFIACNT
                     DAQALLQSDAPHKIRIGDKITRGLGAGGDAAIGARAAEEDSEKIAQALADSDMVFITA
                     GLGGGTGSGAAPIVAQLAKDAGALTIGVVTKPFSFEGAKRKLIAEKAAEELKAQVDTL
                     ITIPNDRLKDVVQKNTSIIDAFRVVDDVLRQGVQGISDIITMPGLINLDFADVRAIMK
                     DAGSALMGIGRASGENRAVEAARMAIASPLLEVNITGAQGILFNITGSSNLSLFEVTE
                     AAEEIRAAADAEANIIFGTSFNDRLGEEVMITVIATGFDGSRKRDTTRHGQAAQPAAD
                     QHSIRAPRERDFLEELERQRIESSDGSADHGREERPAAAVMAPRVERTVGEAVPVKRP
                     AAYDADDLEIPSFLRRK"
     gene            3686..4357
                     /locus_tag="VHM48_10215"
     CDS             3686..4357
                     /locus_tag="VHM48_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755828.1"
                     /translation="MGLQYRAIRAAASVLLVAAAVGTGGMAGMTSTLAADPAMVALEG
                     TVVDGDGTPLAGIHLVISEELPPDGGIAAFQTMTGADGTFAAEVYAWGTADAPASLAI
                     TTPADETVEIVGKTCSQTWGVELDADQEVGLADGAPAPLLLTATTTLLGEVCGTTATP
                     PPDRQGAGGGQPGLTPPPTDTFVAPIEGSPDRLGPALTIGFMVGLLAAAALMLRPHPG
                     SRRRD"
     gene            complement(4529..4942)
                     /locus_tag="VHM48_10220"
     CDS             complement(4529..4942)
                     /locus_tag="VHM48_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX2755829.1"
                     /translation="MNSIRLQVGTGSERGFTDLTGACRRFVAERAGDADGLLSVFVPH
                     ATAGLVILELGAGSDDDAMAALDRLLPRDDRWRHRHGSAGHGADHVLPLLVSPSLTVP
                     VIGGELALGTWQSIALLDPNADNPERTVLLSFLAG"
     gene            complement(5015..6247)
                     /gene="ftsA"
                     /locus_tag="VHM48_10225"
     CDS             complement(5015..6247)
                     /gene="ftsA"
                     /locus_tag="VHM48_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX2755830.1"
                     /translation="MEREAVLVGIDVGTSKVCTLIGEVSRDGRMTVMGHGTVVSSGLK
                     KGVVVNIDQTVRSIAESVERAERLSGWKIDRAFVAVGGQHVESLNSPGQVAVSGPRKE
                     VSREDVNRAVEVARAVSIPSSQVVLHVERRGFKVDGQEGVKDPLGMSALRLEVEVHIV
                     SGAATAVQNLSKCVNAAGVKIDELVASGLASADAVLSETEKELGVAVADIGAGTIDLA
                     MFSDGSPFRTAVLPIGGNNVTNDVALGLKTSLQVAEELKIQHGNCNLAAVEDDEEITV
                     SVLGEDAGRTLQRRELCEIIEARMRETFELLRTEMARGGAGLLPAGIILTGGGAQLAG
                     TAELARDVLGMPVRIAAPTGIGGLVDTILNPGYSTAVGLLLWGASTLAEDVPVRYESA
                     PAMGGLGRIRDALRSIFP"
     gene            complement(6317..6640)
                     /locus_tag="VHM48_10230"
     CDS             complement(6317..6640)
                     /locus_tag="VHM48_10230"
                     /inference="COORDINATES: protein motif:HMM:NF018632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX2755831.1"
                     /translation="MFLPLAGLLLGVLAGLVLNVNVGFEFTRYTAVAILAAMDSVLGA
                     VRAELDGVYDNRIFISGFLTNAIVAVILTFIGDRLSLDLYLVALINFGFRIFNNVALI
                     RRHFV"
     gene            complement(6717..7577)
                     /locus_tag="VHM48_10235"
     CDS             complement(6717..7577)
                     /locus_tag="VHM48_10235"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2755832.1"
                     /translation="MTKGPPRRYRTAAGTPRRQPRVRRASAGLSPIRAAAILAMLISA
                     GSIYGLAATSAFGFERLDVSGLALTSDDAIRTQVGLAPGTNLVGLATEPIAARLRELP
                     SVRDAEVSVGLPDVLQVVVAERRPIIVWAVGDRRFAVDDGGLLFADVTTDPSQATQGT
                     PIVIDERTSAGGLGVASTLDPIDLDAATRLGSLTPSQVGSHASIFRVHVTDDHGFTIS
                     SGSGGWLAIFGFYGRSQRTPALITGQVQALGGLLAGREDTVQTVILADDREGTYIPKP
                     TPRPSATPKP"
     gene            complement(7582..8700)
                     /locus_tag="VHM48_10240"
     CDS             complement(7582..8700)
                     /locus_tag="VHM48_10240"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX2755833.1"
                     /translation="MTSHPPIVLLLGGPSAEHDVSIVSGTAIAEALIGAGDDVRQVLI
                     DLDSGWWWLPRDHRRDGRPGAAYDDPTALGGEGPLTAGAALERLAAADPPPLVFIALH
                     GPFGEDGTVQAMLEAAGLAYTGSGVTASALGMDKAVFKRLVRGIGLPVVDWLEVRSAP
                     WRDDPAAVLAELDAFAAGTGDERLMVKPSGLGSSVGITLAHGAGEYPAALDLAFRHDE
                     VALAEAYLAGARDLEVSVIGNDHAHLELYGPGEILAGHEFYDYAAKYTPGLSETSIRA
                     EVPAPMKASILKIARDAYRAIGAEGFARIDFLVAGERVFLSEINTIPGFTPISLFPTM
                     PADGGYAFADVCARVVDLALERHAARARERLTAADLPR"
     gene            complement(8697..>9737)
                     /gene="murB"
                     /locus_tag="VHM48_10245"
     CDS             complement(8697..>9737)
                     /gene="murB"
                     /locus_tag="VHM48_10245"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX2755834.1"
                     /translation="DRPRGPGMNAAAGETEAPATPPFDPLAIGTAIQRRIGVKTSRDE
                     PLARFTTMRVGGPADLFATVHNIHELRAIVRFARSRGIALTLLGRGSDVVIADAGIRG
                     LVVQNRSEGSRIEGDRYVAESGLPMARAATETQAAGLTGLEFGLAIPGTVGGAVWANA
                     GAHDGDVAGILESADVLLGDGTEARLTAADLEFHYRDSRLKHQPLEVVLAATFRLATA
                     DPATIAARLDDIKRWRREHQPLGVPSAGSTFRNPPGDSAGRLIDALGWKGRTRGGATV
                     SEKHANFIVNDRHGPAADVRGLIDDVAAAVERETGIVLEPEVVFLGDWTAPDPGDAPP
                     AAAANPTTETGR"
BASE COUNT         1437 a         3436 c         3508 g         1356 t
ORIGIN      
        1 cgcgacgaac agcggttgtt caccgccctc gacgggttgc tcggccaggt gacagaggag
       61 caggcggaga cggccctgaa catcctcgcc cccgggtcgg acggcgcgag ccggtaccgg
      121 gagggcttcc ccgccgcggg agcggaggaa ctgtacgaac tggtcaactc cgactgggga
      181 ttccgcatgc cgaccctcca cctcgccgag gcccagatcg cggccggcgg gcgtgcccac
      241 gtctacgagc tgacctgggc cgcgccgggg atgggtggca tcctcggggc ctgccacggc
      301 ctcgacgtgc cgctcgtctt cggcaacgcg gaccgcggtc agcccgccat gctgatcggc
      361 gacagcccga accctgaagc agagatgctg tccgcgcgga tgcgcgccgc gtggacggcg
      421 ttcgccaccc acggcgaccc cggctggatc gcctacgaca cgcagcgacg cctcgtgcag
      481 ctcttcgata cccgcccgac gatcgccgcc gatccggagg agacctcccg tctgatctgg
      541 caggaccacg cctttcctgc cctgccgttg atcggccggt agcacctcct ggaagcagag
      601 agccgcagac agaaacaggg cccgaaggcc ctgcacctgt tggggtcacg atggtgggcg
      661 atactggatt cgaaccagtg accccgcgga tgtgaaccgc gtgctctaac cactgagcca
      721 atcgcccgaa ggaccgcgac gccgctcagg acgtccggta caacccgccg atcatacccg
      781 caggcccgga tagcgggcaa tgagcgccgc cggctcgagg ccgtcgatca cgaccagctt
      841 ctggcgtccg gccgcgcccg cgaccaggcg gacggagcgg cgcggcacgc cgagctcctc
      901 ggcgagcagt cgcagcaggg actggttggc cgcaccgccc accgccgggg ccgcgacgtg
      961 cgcccggagc acgccgtcga ccacgccgtc gacgtggtcg gacccggatc ggggagtcaa
     1021 tcggacggcg aaacggacgt cgatgtcagc caggactcac acgacctgcc acaggatgct
     1081 caggacgacg aggacgacga ggggcgacag gtcgagcgcg cccgtcggcg gcaggacacg
     1141 tcggatcggg gcgaggatcg gctcggtggc ggagatcacg aaggatccca ccggtgaccg
     1201 tcccgccggg tcgaaccacg aaaggaagac gcggccgagc acgagcaacc agaccgcgag
     1261 aaaggcgatc tggagcaggt tcgcgacgag aacgcccacg acccgagtct accagccctt
     1321 gtcgcgccga cccgccagaa gcccatgcgg ccgccggctc acggatgatg ccgctcgccg
     1381 aacagtgccc ggccgacccg cacgatcgtg gcgccctcct cgatcgcgac ctggtagtcg
     1441 tcgctcatgc ccatcgaaag cgccgggcca agcgcgggcg agccaccccg gtcgagctgc
     1501 cgtagacgca ccgacagttc gcggagcgcg acgaaggtcg accgggcatc ctcggcccgc
     1561 tcgacgagcc ggccgacggt catcagtccg cgcagctcga gatgctcgag atccgcgagt
     1621 cgcggcagcg ccgcggccag accgtcgggg tcgaacccgg acttggcggc gtcagcatcg
     1681 acgttgacct ggagcaggac cggcaggcgc tcgcccggcc ggacctcgtt ggccatccgg
     1741 tcgagacgtt cggcgagctc gaccgagtcg accgactcga tgacggcgaa ggccgcgacc
     1801 gcccgcctgg ccttgttcga ctggaggggc ccgatcatgt gccaggtcgc gccatcgacg
     1861 acgccgacct tcgtctccgc ttcctggacg cggttctcgc cgagcgtcgt caggccgacc
     1921 gcgactgctg cgacgaggcg ctcggcgggg accgtcttcg atacggcgac cagcgtgatg
     1981 tccgccggat cgcgcccgac gcgggcgcac gcgtcggcga tgttcgcgag cacacgctcg
     2041 cgggcggccg cgaagcccga gagatcagca gtggcgggcg cgaccatggg cccgcagtgc
     2101 tacttccggc ggaggaagct cgggatctcg agatcatcgg cgtcgtaggc cgccggccgc
     2161 ttgacgggca cggcctcacc gaccgttcgc tcgacgcgcg gcgccatgac ggctgccgcc
     2221 ggtcgctcct cgcgaccatg gtcggccgag ccatcgctcg actcgatccg ctggcgctcg
     2281 agctcctcga ggaaatcgcg ctcgcgcggg gcgcggatcg agtgctggtc ggcggccggc
     2341 tgggcagcct ggccgtggcg ggtcgtgtcg cgcttgcggc tgccatcgaa gccggtggcg
     2401 atgacggtga tcatcacctc ctcgccgagc cggtcgttga agctggtccc gaagatgatg
     2461 ttcgcttccg cgtcggccgc ggcccggatc tcctcggccg cctcggtgac ctcgaacagg
     2521 ctgaggttcg acgacccggt gatattgaac aggatgccct gggcgccggt gatgttgacc
     2581 tcgagcaggg ggctcgcgat cgccatccgg gcggcctcca cggcccggtt ctcgccgctc
     2641 gcccggccga tccccatcag ggccgatccg gcgtccttca tgatcgcccg gacgtcagcg
     2701 aagtcgaggt tgatcaggcc cggcatcgtg atgatgtcgc tgatcccctg gacgccctgg
     2761 cggaggacgt cgtcgaccac gcggaacgca tcgatgatcg aggtgttctt ctggacgacg
     2821 tccttgagtc ggtcgttcgg gatcgtgatg agcgtgtcga cctgggcctt gagctcctcg
     2881 gccgccttct ccgcgatgag cttgcgcttg gcgccttcga agctgaacgg cttggtgacg
     2941 acgccgatcg tcagtgcgcc ggcatccttc gcgagctggg cgacgatcgg ggcggcgccg
     3001 gagccggttc cgccaccgag cccggcggtg atgaacacca tgtccgagtc ggccagggcc
     3061 tgggcgatct tctccgaatc ctcctcggcc gcccgcgctc cgatcgccgc atcgccgccg
     3121 gcgccgaggc cgcgggtgat cttgtcgccg atccggatct tgtgcggcgc atccgactgg
     3181 aggagagcct gcgcgtccgt gttgcaggcg atgaactcga cgcccatcat ctcggcccgg
     3241 atcatccggt tgacggcgtt gctgccgccc ccgccgacgc cgatgacgcg aatgagcgcg
     3301 aagttctcgg aatcggaccg caacgccatc gtcgtcctcc ggggtctgta gctcgtggag
     3361 acggaccatc cagcgggaat ggcgaccgtc gggggtggtt tgcgaggcag acccgtggac
     3421 cacgcatcgt ctgcgttcct ctggtccctc gggctgccgg gagtctacca ccgggccgtc
     3481 gggatcgggc accgggatcg gcgcccgccg gccgatccgg aggcgctcgt ccgcctgagg
     3541 tccaccgcga accgttgacc aggtgcaccg tgggaggtgc gccagccgac cgtccggcga
     3601 tagggcatgc gccctatgga cacgctcggg cgggcgctgc gatcctgccg ggcgtaccgg
     3661 cactggtact tcggggagag cgcgcgtggg acttcagtac cgagcgatcc gtgcggcggc
     3721 gtccgtcctg ctggtcgctg ccgccgtggg caccggcggc atggccggca tgacgtccac
     3781 gctggcagcc gacccggcga tggtcgctct cgaaggcacg gtcgtcgacg gcgacggcac
     3841 gccgctcgcc ggcatccacc tggtcatctc cgaggagctg ccgcccgacg gcggcattgc
     3901 cgcattccag acgatgaccg gggccgacgg cacctttgcg gccgaggtct acgcctgggg
     3961 gaccgccgac gcaccggcca gcctcgcgat cacgacaccc gccgacgaga cggtcgagat
     4021 cgtcggcaag acctgcagtc agacctgggg tgtcgagctt gatgccgacc aggaggtcgg
     4081 ccttgccgac ggagcgccgg ccccgctcct cctgacggcc acgaccaccc tgctcggcga
     4141 ggtctgcggc acgaccgcga caccgccgcc cgacagacag ggcgcggggg gcggtcagcc
     4201 cggcctgacc ccgccgccga ccgatacctt cgtcgcgccg atcgaaggct cgccggaccg
     4261 tctcgggccg gccctgacga tcgggttcat ggtcgggctc ctcgcagccg cggccctgat
     4321 gctccggccg cacccggggt cgcgccgccg ggactgatcc ccgcccggat cgactccgtg
     4381 tgaacgagcg tggtccggtc gcagttctga cgggatccgg cggaatcggc gatctggtcg
     4441 tgctcattcg aaacggactg gtccagctgg gcatcacgcg cgccatcggg ctcgccgccc
     4501 ggaccacgga tcgctacccg cgagatctct accccgcgag gaagctgagc agcaccgtcc
     4561 gctcggggtt gtccgcgttg gggtcgagca gggcgatcga ttgccatgtc ccgagggcca
     4621 gctcgccgcc gatcacgggc accgtcagcg acggcgagac gagcagcggc aggacgtgat
     4681 ccgcgccgtg gcccgccgag ccgtggcgat gccgccagcg atcgtcgcgc ggcagcagcc
     4741 ggtcgagcgc ggccatcgca tcgtcgtcgg acccggctcc gagctcgagg atcacgagcc
     4801 cggccgtcgc gtgcggcacg aacaccgaga gcagaccgtc cgcatccccc gcccgctcgg
     4861 ccacgaaccg ccggcacgcg ccggtcaggt ccgtgaaccc gcgttcggac cccgtcccga
     4921 cctggagcct gatggagttc atgcgcagct ctccgaggtc cgaggccgag ctccgccagg
     4981 ccatcggccc gaggaactcc gcgaagccat tgccctaagg aaagatgctg cgcagggcgt
     5041 cgcggatccg gccgaggccg cccatcgccg gcgccgactc gtaccgcacc ggcacgtctt
     5101 cggccagcgt cgatgcaccc cacaggagca gcccgaccgc cgtcgagtag cccgggttca
     5161 ggatcgtgtc gacgaggccg ccgatcccgg tcggggcggc gatccggacc ggcatgccga
     5221 ggacgtcgcg ggccagctcg gccgtgcccg ccagctgcgc cccgccgccg gtcaggatga
     5281 tcccggccgg cagcagtccg gcgccgccgc gggccatctc ggtccgcagc agctcgaagg
     5341 tctcccgcat ccgggcctcg atgatctcgc acagctcacg tcgctggagc gtccggccgg
     5401 catcctcgcc gaggacgctg accgtgatct cctcgtcgtc ctcgaccgcg gcgaggttgc
     5461 agttcccgtg ctggatcttc agctcctcgg cgacctggag gctcgtcttg aggccgagcg
     5521 cgacatcgtt cgtgacgttg ttcccgccga tcggcaggac cgcggtccgg aacggcgagc
     5581 cgtcggagaa catcgcgaga tcgatcgtcc cggcaccgat gtcggcgacc gcgacgccga
     5641 gctccttctc ggtctcggac agaaccgcgt cggccgacgc gaggcccgag gcgacgagct
     5701 cgtcgatctt gacgccggca gcgttgacgc acttggagag attctggacg gcggtcgcgg
     5761 cgccgctgac gatgtggacc tcgacctcga ggcggagggc gctcatcccg agcgggtcct
     5821 tgaccccctc ctggccgtcg accttgaagc cgcgccgctc gacatggagg acgacctggc
     5881 tcgacgggat cgagacggcc cgggcgacct cgacggcgcg gttgacgtcc tcgcggctga
     5941 cctccttgcg ggggccgctg accgcgacct ggccaggcga gttgaggctc tcgacatgct
     6001 ggccgccgac ggccacgaac gcccgatcga tcttccagcc cgacagccgc tccgcccgct
     6061 cgaccgactc cgcgatcgag cggaccgtct ggtcgatgtt gacgacgacg cccttcttca
     6121 ggccggacga gacgaccgtg ccgtggccca tgaccgtcat ccggccgtcg cggctgacct
     6181 cgccgatcaa ggtacagacc ttggacgtgc cgacatcgat cccgaccagg accgcttctc
     6241 tctccaccgt cgtcgtccgt gcctcctgcg ttggccgccc gtccgcgtcg cggaccgggc
     6301 cggcggtcga tgtttcctag acgaagtggc gccggatgag cgccacgttg ttgaagatgc
     6361 ggaacccgaa gttgatcagg gccaccaggt acaggtccag gctgagccga tcgccgatga
     6421 acgtgaggat caccgcgacg atcgcattgg tgaggaagcc actgatgaag atccgattgt
     6481 cgtagacgcc gtcgagctcg gcccggaccg cgccgaggac cgaatccatc gcggcgagga
     6541 tcgccacggc cgtgtagcga gtgaactcga aaccgacgtt gacattgagg acgagcccgg
     6601 ccagaacgcc caggaggaga cccgccaagg gtaggaacac gcctcgccgc cctcattccg
     6661 gtgcgtttgg gcaaggctac ccgacacacg cggcggctgc cagccgagcc gatcgatcac
     6721 ggtttcggcg tcgcgctggg gcgaggggtc ggcttcggga tgtaggtccc ttcccggtca
     6781 tcggcgagga tgacggtctg gacggtgtcc tcgcggccgg ccagcagccc gccgagggcc
     6841 tggacctggc cggtgatcag ggccggcgtc cgctggctgc ggccgtagaa accgaagatc
     6901 gccagccagc cgccgctgcc gctgctgatc gtgaagccgt gatcgtcagt gacgtggacc
     6961 cggaagatcg acgcatggct gccgacctgg ctcggggtca gcgacccgag gcgggtcgcc
     7021 gcgtcgaggt cgatcgggtc gagggtcgag gccaccccaa gaccgcccgc cgatgttcgc
     7081 tcgtcgatca cgatcggcgt gccctgggtc gcctgcgacg ggtcggtcgt gacgtcggcg
     7141 aacaggagcc cgccgtcgtc gacggcgaac cggcgatcgc cgacggccca gacgatgatc
     7201 gggcgacgct cggccacgac cacctggagc acgtccggca ggccgacgga aacctccgcg
     7261 tcgcgaaccg atggcagctc ccggaggcgg gccgcgatcg gctcggtggc caggccgacg
     7321 aggttggtgc ccggcgcgag cccgacctgg gtccggatcg cgtcgtcgct cgtcagggcc
     7381 aggccgctca cgtcgagccg ctcgaaaccg aatgccgagg tggcggcgag gccgtagatc
     7441 gaccccgccg agatcagcat cgcgaggatc gccgccgccc ggatcggtga caggccggcc
     7501 gacgcgcggc ggacgcgcgg ctggcgacgc ggggtgccgg ccgcggtgcg atagcgacgg
     7561 ggcgggccct tggtcatcgg ctcagcgtgg caggtcggcg gccgtcagcc gctcgcgggc
     7621 ccgggcggca tggcgctcga gggcgaggtc cacgaccctg gcgcagacgt cggcgaaggc
     7681 gtagccgccg tcggccggca tcgtcgggaa gaggctgatc ggggtgaaac ccgggatggt
     7741 gttgatctcg gacaggaaga cccgctcgcc cgccacgagg aaatcgatcc gggcgaagcc
     7801 ttcggctccg atcgcccggt aggcatcgcg ggcgatcttg aggatcgatg ccttcatcgg
     7861 cgcggggacc tcggcccgga tcgaggtctc cgacaggccg ggcgtgtact tggccgcgta
     7921 gtcgtagaac tcgtggccgg cgaggatctc gcccgggccg tagagctcga gatgggcgtg
     7981 gtcgttgccg atgacgctga cctcgaggtc gcgggcgccg gcgaggtagg cctcggcgag
     8041 ggcgacctcg tcgtgacgga atgcgaggtc gagcgcggcg ggatactccc ccgccccgtg
     8101 ggccagggtg atcccgaccg acgacccaag ccccgacggc ttgaccatca gccgttcatc
     8161 gccggtgccg gcggcgaacg cgtcgagctc ggcgaggacg gcggccggat cgtcgcgcca
     8221 gggggccgac cggacctcga gccaatcgac gaccggcagg ccgatcccgc ggacgagccg
     8281 cttgaagacc gccttgtcca tgccgagagc ggacgccgtg acccccgagc cggtgtaggc
     8341 gaggccggcg gcctcgagca tcgcctggac cgtgccgtcc tcgccgaacg ggccatggag
     8401 ggcgatgaac acgaggggcg gcgggtcggc ggcggcaagc cgctcgagag ccgcgccggc
     8461 ggtcagcggg ccctctccgc cgagggcggt cggatcgtcg taggcggcgc cgggccggcc
     8521 gtcacggcga tgatcgcgcg gcagccacca ccagccgctg tcgaggtcga tcaggacctg
     8581 gcggacgtcg tcgccggcgc cgatcagggc ctcggcaatg gccgtacctg acacgatcga
     8641 gacgtcgtgc tcggcggacg ggccgccgag gaggaggacg atcggcggat gggaggtcat
     8701 cggccggtct ccgtcgtggg gttcgccgcg gcggccggcg gcgcgtcgcc gggatcgggc
     8761 gcggtccagt cgccgaggaa cacgacctcg ggctcgagaa cgatgccggt ctcgcgctcg
     8821 acggctgcgg cgacgtcgtc gatcaggccg cggacgtcgg ccgccgggcc gtgccggtcg
     8881 ttgacgatga agttggcgtg cttctccgac accgtggcgc cgccgcgggt ccggcccttc
     8941 cagccgagcg cgtcgatcag gcggcccgcc gagtcgccgg gcgggttgcg gaacgtcgag
     9001 cccgccgacg ggacgccgag gggctggtgc tcgcgccgcc agcgcttgat gtcgtcgaga
     9061 cgagcggcga tcgtggccgg gtcggccgtg gcgagccgga acgtcgcggc caggacgacc
     9121 tccaggggct ggtgtttcag ccggctgtcg cgatagtgga attcgagatc ggcggcggtg
     9181 aggcgagcct ccgtgccgtc gccgagcagg acgtcggccg actcgaggat ccccgcgacg
     9241 tcgccgtcat gggcgcccgc gttggcccag acggcgcccc cgaccgtgcc cgggatcgcg
     9301 aggccgaact cgagtccggt caacccggct gcctgtgtct cggtcgccgc ccgggccatc
     9361 ggcaggccgg actcggcgac gtagcgatcg ccctcgatcc gcgagccctc cgagcggttc
     9421 tggacgacga ggccccggat cccggcatcg gcgatgacca cgtcgctgcc ccgcccgagc
     9481 agggtgaggg cgatgccacg cgaccgggcg aagcggacga tcgcccgcag ctcgtggatg
     9541 ttgtggaccg tcgcgaacag gtcggccgga ccgccgacgc gcatcgtcgt gaatcgggcg
     9601 agcggctcat cgcgcgacgt cttgacgccg atccggcgct ggatggcggt tccgatcgcg
     9661 agcgggtcga acggcggcgt cgccggggcc tcggtctcgc cggcggccgc gttcatcccc
     9721 gggccccgcg ggcgatc
//
LOCUS       DATAIW010000333         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_188004, whole genome shotgun sequence.
ACCESSION   DATAIW010000333 DATAIW010000000
VERSION     DATAIW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188004"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..2870
                     /locus_tag="VHM48_10250"
     CDS             150..2870
                     /locus_tag="VHM48_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2755835.1"
                     /translation="MPVSARLLALHAAATIEPTKPGPAVGDDVGPSSRRSVPDHNVAR
                     PMGPIVVIVGGAILSAIFIAFATLDVLDLAGLWELAHWTVSIGAALIATSVAWRAASG
                     HERQIRAWVLIAVGVWAVGRVLAVLDGPLGLMASPSPADLVMVATVVPGIGWLIASVD
                     PRLGRTARTAALLDITAIIVALAGIVVVTFGRTAEEIGGLAGSVALAYPVTFITGAGA
                     VSMVAFMGAGRRTIPISTLAMAAGLALFGLAGIALLADPRSSHGVVGDPVRALVSIAP
                     LAVAAGSAFGRPAIVRVRANTADSRSFLMPIATASFAALVILYLDDRLDGIAEIGMRV
                     AISITIVLIIVRQTMLLDDRRVAAERERVATLRAEAASADAGRIAQLLATAEERFRNL
                     VEQVPAAVYLDRLDLDQLAISETVYVSPRVEGLTGFSPAEIAADNELWFERIHVEDRE
                     QVLTEWDRHCRARTPFSVTYRFLTKDGRTIWLAEDAAILESDGHAFSQGMILDVTDRR
                     ETEASLRQAQKMEAVGQLAGGVAHDFNNLLTVITGHAGLLRETIDVNDPTRIDVDAIA
                     TAAASSADLVGQLLAFGRRTMLRPEIVDIGTVVSDVLPMLRRLLPEHIDVVTRLDPDL
                     PAIRADPGQVQQVVLNLAINGRDAMPAGGTLTIATEARVVSPSEASRHLGLAPGSYVR
                     LTIADEGVGMDAATRDRIFEPFFTTKAAGRGTGLGLATVYGIVKQSGGYIAIDSAVDR
                     GSTFSVFLPVTTEQVAVRPPSIPVRAPRGHERIVLCEDEPAVRDLVAAVLRRSGYSVV
                     SATNPADALLLIADRQSPADLLLTDVVMPGMSGPDLARRASVLRPDLRVVLMSGYALD
                     AADRDAVGRQAVFLAKPFSPSALARTVRDALDDVPGVDGALDLGEVAARG"
     gene            complement(2892..3365)
                     /locus_tag="VHM48_10255"
     CDS             complement(2892..3365)
                     /locus_tag="VHM48_10255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2755836.1"
                     /translation="MAGASDPRDLDGDFGHRTTSEVRFADTDAMGHVNNAVYLTFVEL
                     ARIGWWMAATGEPLEREAGRAEGLILAEAEIAFRSPLFFGETVVVETRASRIGRTSLG
                     LDHRLTASRPGGSVRLVATCRSVIVHYDYVATTPIPWPAELIECIEAFEGRLPRA"
     gene            complement(3392..>3997)
                     /locus_tag="VHM48_10260"
     CDS             complement(3392..>3997)
                     /locus_tag="VHM48_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2755837.1"
                     /translation="TMAVVRENLFWAFAYNVILIPVAMGLLVPFGIALSPALAAAAMA
                     LSSVAVVTNSLRLRSFDARPDAVRRAPGRGPVARLRRGWYLVAVAVASLTLAGGVIAA
                     DRAIDAGAVSLEVHAHDVRFEPADLSVPAGRFVVLRFTNDDPVFHDWMIEGLANVDAG
                     ARPGQTQRLRFRIDTPGTYHIVCSVEGHAEAGMVGTLVVTP"
BASE COUNT          568 a         1446 c         1389 g          594 t
ORIGIN      
        1 cccgcgaccc ccgcgaccga gggtcgccgc ggggctcgcc gccgccatcg ggcgcgaccc
       61 tgcgaccata tgcctacgtc gttcggatgg ccggtccacc cccaaacggg gagtgccgga
      121 tcacctgtcg ggggcctatc gtcgggaaca tgcccgtatc cgcgcgcctg ctcgcgctcc
      181 acgccgccgc gacgatcgag ccgaccaaac ccgggccggc ggttggcgac gatgtcggcc
      241 catcctccag gcgctcggtc ccggaccaca acgtcgcccg acccatgggg ccgatcgtcg
      301 tcatcgtcgg cggcgccatc ctcagcgcga tcttcatcgc cttcgccacg ctcgacgtcc
      361 tcgacctggc cggactgtgg gagctggctc actggaccgt ttcgatcggg gccgcgctga
      421 tcgccacgtc cgtcgcctgg agagcggcct cgggccacga gcgccagatc agggcgtggg
      481 tcctgatcgc ggtcggcgtc tgggcggtgg gacgcgtcct ggccgtgctc gatggaccgc
      541 tcgggctgat ggcctcgccg agtcccgcgg acctcgtgat ggtcgcgacc gtggtcccgg
      601 gcatcggctg gctcatcgcg agcgtcgatc cccggctcgg gcgaaccgcg cggaccgcgg
      661 ccctgctcga catcaccgcg atcatcgtcg ccctcgccgg catcgtcgtc gtcaccttcg
      721 gccggaccgc cgaggagatc ggcggactcg ccggttccgt ggcgcttgcg tatcccgtga
      781 ccttcatcac cggtgccggt gcggtgagca tggtcgcctt catgggcgcc ggtcgccgca
      841 ccatcccgat ctcgacgctg gccatggccg ccgggttggc gctgttcggc cttgccggga
      901 tcgccttgct ggccgacccg cggtccagcc acggcgtcgt gggcgatccg gtccgggcgc
      961 tggtctcgat cgcgccgctt gcggtcgcgg ccgggagcgc gttcgggcga ccggccatcg
     1021 tgcgggttcg cgcaaacacc gcggattcga ggagcttcct catgccgatc gcgacagcct
     1081 cgttcgcggc tctcgtgatc ctgtacctcg acgaccggct cgacgggatc gccgagatcg
     1141 ggatgcgcgt cgcgatctcg atcacgatcg tcctgatcat cgtccgccag acgatgctcc
     1201 tggacgaccg gcgcgtggct gccgaacggg agcgggtggc aacgctccgg gccgaggccg
     1261 catcggccga cgcgggtcgg attgcccagc tcctggctac cgccgaggag cgattccgca
     1321 acctcgtcga acaggtgccc gcggcggtct atctcgaccg gctcgatctg gaccagctcg
     1381 cgatctcgga gaccgtctac gtcagcccgc gggtcgaggg gctgaccggt ttcagcccgg
     1441 ccgagatcgc ggccgataac gagctttggt tcgagcgcat ccacgtcgag gatcgggagc
     1501 aggtgctcac cgagtgggac cgacactgcc gtgcgcgtac tccgttcagc gtcacctatc
     1561 gattcctgac gaaggacggc cgcacgatct ggctcgccga ggacgccgcc atcctcgaat
     1621 cggatggcca cgccttctcg cagggcatga ttctcgacgt gacggaccgg cgggagaccg
     1681 aggcgtcgct ccgccaggct cagaagatgg aggccgtcgg ccagctcgcc ggcggcgtcg
     1741 cccacgactt caacaacctg ctgaccgtga tcacgggcca cgccggactt ctgcgcgaga
     1801 ccatcgacgt caacgacccg acgcggatcg acgtcgacgc gatcgcgacc gccgccgcca
     1861 gctcggcgga tctcgtcggg cagctcctcg cattcgggcg acggacgatg ctgcggcccg
     1921 agatcgtcga catcgggacg gtcgtgagcg atgtcctgcc gatgctccgg cgtctcctgc
     1981 ccgagcacat cgacgtcgtc acccggctcg atcccgacct cccggccatt cgcgccgacc
     2041 ccggtcaggt ccagcaggtc gtgctcaacc tggcaatcaa cggacgggac gcgatgccgg
     2101 ccggcgggac cctgacgatc gcgaccgagg cacgggtcgt ctcgccgtcg gaggcgagcc
     2161 gccacctcgg cctcgcgccc ggcagctatg tccggctgac gatcgccgac gagggcgtcg
     2221 gcatggatgc cgcgacccgc gatcggatct tcgagccgtt cttcaccacc aaggccgccg
     2281 ggcgcgggac aggtctcggc ctcgcgaccg tgtacggcat cgtcaagcag agcggtggct
     2341 acatcgcgat cgacagcgcc gtcgatcgcg gctcgacgtt cagcgtgttc ctccccgtca
     2401 ccaccgagca agtcgccgtc cggccgcctt cgatcccggt ccgggcgccg cgcggtcacg
     2461 agcggatcgt cctgtgcgag gacgagccgg ccgtccgcga tctcgtggcc gcggtccttc
     2521 gccgctccgg ctacagcgtc gtgagcgcca cgaacccggc cgatgcgctg ctcctgatcg
     2581 ccgatcgcca gtcgcccgcc gacctgcttc tgaccgatgt cgtcatgccc ggaatgtccg
     2641 ggccggacct cgctcgtcgt gccagcgtgc tgcgacccga cctgcgggtg gtcctgatgt
     2701 cgggctacgc gctcgacgcc gccgaccgcg acgccgtggg gcgacaggcg gtattccttg
     2761 ccaagccctt cagtccgtcg gcgctggccc gaacggtgcg cgacgcgctc gatgatgttc
     2821 ccggggtcga cggcgcgctc gatctcggcg aggtcgcggc acgaggctga gctcggctgg
     2881 cgatccctgc gtcaggcccg cggcaagcgg ccctcgaacg cctcgatgca ctcgatcagc
     2941 tcggccggcc aggggatcgg ggtcgtggcg acgtagtcgt agtggacgat cacgctccgg
     3001 cacgtcgcga cgagtcggac cgagccgccc ggacgggatg ccgtgaggcg atggtcgagt
     3061 cccagactgg tccggccgat gcggctcgcc cgcgtctcga cgacgacggt ctccccgaag
     3121 aacaggggcg aacggaaggc gatctcggcc tccgcgagga tcaggccctc ggctcgaccg
     3181 gcctcccgtt cgagcggctc gccggtcgcg gccatccacc agccgatgcg cgccaactcc
     3241 acgaacgtca ggtagaccgc gttgttgacg tgacccatcg cgtcggtgtc ggcaaagcgg
     3301 acctcgctgg tggtgcgatg gccgaagtcg ccgtcgaggt cgcgcgggtc ggacgcgccc
     3361 gccaccgcca ctcgcccacg cggcgccgcg gtcacggcgt cacgaccagg gtgccgacca
     3421 tgcccgcctc ggcatggccc tcgaccgaac acacgatgtg gtaggtgccg ggcgtgtcga
     3481 tccggaaccg gagccgctgc gtctggcccg gtcgggcgcc cgcatccacg ttcgcgagcc
     3541 cctcgatcat ccagtcgtgg aacacgggat catcgttcgt gaaccggagg acgacgaagc
     3601 ggccggccgg cacggacagg tcggccggct cgaaacggac gtcgtgggca tgaacctcga
     3661 gggacaccgc gccggcgtcg atcgcccggt cggcggcgat gacgccgccg gcgagggtca
     3721 atgaggcgac cgcaacggcg acgaggtacc agccccgccg gagccgggcg accgggcccc
     3781 gtccgggtgc tcggcgaacg gcatccggcc gggcgtcgaa cgagcgcagc cgaagcgagt
     3841 tggtgacgac cgccaccgac gaaagggcca tggcggccgc ggcgagggcg gggctcaggg
     3901 cgatcccgaa cgggacgagg agacccatcg cgaccgggat gaggatcacg ttgtaggcga
     3961 aggcccagaa caggttctcg cggaccacgg ccatcgt
//
LOCUS       DATAIW010000334         9771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_21115, whole genome shotgun sequence.
ACCESSION   DATAIW010000334 DATAIW010000000
VERSION     DATAIW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21115"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VHM48_10265"
     CDS             <1..949
                     /locus_tag="VHM48_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HEX2755838.1"
                     /translation="AHALAEGERVGYPLLIKPAAGGGGKGMRMVRTPRELEASLNVCR
                     SEARAAFGDDSLYLECWLDETRHVEVQVAVDRYGRGIHLGERDCSVQRRHQKILEEAP
                     TPSLSVEARNELCTRAVRAVVSAGYENVGTLEFLVDRDGSFYFIEINCRIQVEHPVTE
                     MLTGIDMVAMQIRIAAGEPLGFSQADIVSRGHAIEFRINAEDPEREFRPAAGVVERYL
                     APGGPGVRMDSHLYAGYEVPPFYDSLLGKLIVWGPTRQAAIERGHVALAELMVEGLVT
                     NVPIHQALLSSEAFLEGRMTTNLLDRVGAAAFLAAAARA"
     gene            1245..1775
                     /gene="fabZ"
                     /locus_tag="VHM48_10270"
     CDS             1245..1775
                     /gene="fabZ"
                     /locus_tag="VHM48_10270"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX2755839.1"
                     /translation="MTDAATGTPPKPSLPKPSLPKLTTPKLTTIHSTAEIQALIPHRW
                     PILLVDRIVEYDAEAKRIVGIKGVAATEWFFQGHFPGLPVMPGVIQVEALAQTMAVYV
                     AKQPGFGDRIGLFAGIDDCRFKRVVVPGDVLRLEITMEKLGSRFGRAKAVASVDGEVS
                     CEATLSFVIPGEGVLR"
     gene            1772..2518
                     /locus_tag="VHM48_10275"
     CDS             1772..2518
                     /locus_tag="VHM48_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2755840.1"
                     /translation="MTARVAIISDVHGNLLALTAVRAALKKDRPDHVVIAGDHVFQGP
                     DPAGVVDALREMEANGATIIQGNTDVAVADFDYGAAFPWFTDGVPDMVRDAAEWAHDA
                     LGDERVSWLRRLPSERRLMLDDTMLLGCHASPGSQTQGFDIQLDPNVILERISRTDAR
                     LICCGHTHLPEIRDLGWKMIINAGSAGYVFDGDPTASWALVEVDGTEVRAEIRRTEFD
                     TMIVANAISERGLVGDVYRAATVRTGKLVR"
     gene            2532..3821
                     /gene="fabF"
                     /locus_tag="VHM48_10280"
     CDS             2532..3821
                     /gene="fabF"
                     /locus_tag="VHM48_10280"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:TIGR03150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX2755841.1"
                     /translation="MTEQRRPNPDWPAEPRRVVVTGMGMLTALGRDVATTWAGMVAGR
                     SGVAPITLFDPERLGCRIAGEVKDFDSSHVLDKKEMRRTDRYIQLGLVAAREAMDQAG
                     LPERMEGEEAERTGVIFGTGLGGVGTLVEGISINALRGPDRISPFFIPMGIPNVGAGM
                     IAISFGMTGPNFTTVSACATGGHAIGEAWETIRRADADMMVAGGAEAGIREPLVGGFD
                     SMHALSRRNDDPEGASRPFDQGRDGFVPGEGSGALVLEELEHARARGATIYAEIIGYG
                     ATADASHITLPAPGGIGAVRAAERAMRKAGVVPADVVHVNAHATSTPEGDRAELQAIR
                     TTFGEHAGSVTVTSNKSMIGHTLGAAGAIEAIVTIQTIRESCVPPTINLIDPDPAGEG
                     LDLVANVARRQDVPIALSNSFGFGGQNTALIIRRWTE"
     gene            3818..4474
                     /locus_tag="VHM48_10285"
     CDS             3818..4474
                     /locus_tag="VHM48_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2755842.1"
                     /translation="MTEHDEEVDPPIELDHEHDADGNVVPRPGLTNGDGGGVDPAGAA
                     ARPDLDPGDASLLALVDRLGSLLDRSELSELAVEAGGTRLVLRTPAAIATTVIAGPAW
                     SGEPDAGAALAASATGGGSDASTVGSAQTGGRTEAPARPSVKAPLTGVWYSSPAPGSA
                     PFVAVGREVAVGQVIGLIEAMKLFNEIKSDLAGRVVRVVPESGALVKAKQPLIEVEPL
                     "
     gene            4471..5694
                     /locus_tag="VHM48_10290"
     CDS             4471..5694
                     /locus_tag="VHM48_10290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX2755843.1"
                     /translation="MNDARPAGTAASTTTRAHVTGWGRYAPAHVLTNADLEKLVDTSD
                     EWIQSRTGIRERRVAAAHETTASMAAVAGLRAIRTAGLEPDDIDLILLATLTPDYWMP
                     STAALVKEAIGNTKATAFDVMAACSGFVYGFATAQAYIQAGLAKHVLVIGAELLTRFL
                     DYTDRGTCILFGDGAGAVVLSASDEPGGALGVEMTTEPRGAYMIWLPAGGSKAPPSAG
                     TISRGEHYVRMEGRETYRFATKTMATTALESIRRSGLEPGDIDLFIPHQANVRIIEAV
                     AKGLDLPMDKMFINLDKYGNTSAASVPIALAEAVNEGRVAIGDNVTIVAFGAGFTSGA
                     VTIEWTADPARGPIGDAAVRPEDVNVRLPVDWDSVDPIPEALAEIMRRPGPVDVPLDD
                     VVPGEPEPAHREVHA"
     gene            5691..6440
                     /gene="fabG"
                     /locus_tag="VHM48_10295"
     CDS             5691..6440
                     /gene="fabG"
                     /locus_tag="VHM48_10295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX2755844.1"
                     /translation="MIDLSGKIALVTGGSRGIGKAIALRLATQGADVAFSYRGNEAAA
                     ASTTAEIEGLGRRALAVQADVSDADAAAGLVKAVLEAFGKVDILVNNAGITRDDLIMR
                     MSVEAFREVLETNLFGAFYMTKAVTRPMLKAKGGRIVNITSVSGQAGQTGQANYSAAK
                     AGLIGLTKATARELASRTITCNAVAPGFVLTELTKDLPEALQSQITAATPLGRFGTTD
                     EIANAVAFLASDEAAFITGQVLAVDGGLVMM"
     gene            complement(6481..6897)
                     /locus_tag="VHM48_10300"
     CDS             complement(6481..6897)
                     /locus_tag="VHM48_10300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2755845.1"
                     /translation="MHTTASFDIPVPPDRVVAYLSRPRNLLIANHVGPVIEQSDPPVR
                     RGSWSVHQFDQLRVRVEYIALERDEVAVAISSTGRGSGGVNGSFRYWLAPVDASRGTR
                     VSVEAETPRGLIRDPIGRLLWPLLWHRIRDRMAREA"
     gene            7205..8701
                     /locus_tag="VHM48_10305"
     CDS             7205..8701
                     /locus_tag="VHM48_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2755846.1"
                     /translation="MQIRQFRVEGLGHLSTLVADETAGVAAVVDPRRDVDCYLAAARE
                     LDVRITHVVETHLHNDYVSGGRDLAALTGSRHVIGAGAELRHEHGPVRDGETFDVGSL
                     RFTSHDTPGHTPEHVAYSVADLDRGEDPALLFSGGSLLVGAVGRTDLLGEEHARDYAR
                     AMHRSLHEVVLAHDDHVAVHPTHGAGSLCATGIASTPTTTIGYERRHNALLRVTDVEA
                     FATRLLRGQPAVPHYFARMRPTNQAGPVLLGGRVPEPRPLSLDGARSALAAGALLLDL
                     RPPADHAVSHVPGSLSIPLGPSFGTWLGWVVEPARPLVLILERTDDWDEAVRQALRIG
                     AEGSIVGHLRGGLGTWSDAGAPLESNGRLSVDELAATIERAAPADAPLVIDVRQANEY
                     ETGHVPGSIHIAAGSLPDRLAELPRQRPIATICASGLRSSIAASILRSAGFDDVSWVA
                     SGVSAWRAAGHPTVRGDQPTRPTATSRPGAAGTGARAGRDAGTVAAHD"
     gene            8832..>9771
                     /locus_tag="VHM48_10310"
     CDS             8832..>9771
                     /locus_tag="VHM48_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2755847.1"
                     /translation="MSAHPPTRPRRRDTRASGRPSGARSLAAPGIAPAGTSTVTPALA
                     SPVRATDESIAALFARQLEVLQAASARLSRAEGSEAVGRAVVEETGRIIDYHNARVYV
                     LEAGGDLVPIAFEGRVGEYEKVDLELLRTRLGVGFTGWVGLHGEPLLVRDANDDPRGA
                     SIPGTDDVDESMLVVPMRYDDAVVGVITLSKLGLDQFDGHDLRLLSILADQAATALES
                     ARNLARSDALAGELRLLLDVSSQLAVSLDSHQVANILARHLATVMDVEECAISWWDRP
                     HDRVASIGYYPPVPVAELTPFFDISDYPSTKHVLEDQ"
BASE COUNT         1469 a         3588 c         3374 g         1340 t
ORIGIN      
        1 cgcccacgcc ctcgccgagg gggagcgggt cggctacccg ctcctgatca agccggcggc
       61 gggcggcggt gggaagggca tgcggatggt ccggacgccg cgcgagctcg aggcctcgct
      121 caacgtctgc cgctccgagg cgcgtgccgc attcggcgac gattcgctct acctcgagtg
      181 ctggctcgac gagacgcgcc acgtcgaggt ccaggtcgcg gtcgaccgct atggccgagg
      241 catccacctc ggcgaacgcg actgcagcgt ccagcgccgt caccagaaga tcctcgagga
      301 ggccccgacg ccgtcgctct cggtcgaggc ccgcaacgaa ctctgcaccc gcgccgtccg
      361 ggccgtcgtg tcggccggct acgagaacgt cggaaccctg gagttcctcg tcgaccgcga
      421 cggcagcttc tacttcatcg agatcaactg ccgaatccag gtcgagcacc ccgtcaccga
      481 gatgctgacg gggatcgaca tggtcgcgat gcagatccgc atcgctgccg gcgagccgct
      541 cggcttcagc caggccgaca tcgtgagccg cggtcacgcg atcgagttcc ggatcaacgc
      601 cgaggatccc gagcgcgagt tccggcccgc cgccggcgtc gtcgagcggt acctcgctcc
      661 cggcggcccg ggcgtccgca tggactccca cctctacgcc gggtacgagg tgccgccctt
      721 ctacgactcg ctcctcggca agctcatcgt ctggggtccg acccgccagg ccgcgatcga
      781 gcgcggccac gtcgcgctcg ccgagctgat ggtcgaaggc ctcgtgacga acgtcccgat
      841 ccaccaggcg ctcctgtcca gcgaggcgtt cctcgagggt cggatgacga cgaacctgct
      901 cgaccgggtc ggcgccgccg ccttcctcgc cgcggcggcc cgggcgtgag cacctcggcc
      961 ggccagtggt ccgccagccg gaccaacggg cgaacgagca ccgccgcggg cccgtccgga
     1021 cacgttccgg cctcccattg ggccgccagc cggaccaacg agcgaacgag cgccgccgag
     1081 cgccgatcga gatgcgtccc ggcctctcct tggtccgcca ggcggaccga gggcacgtcg
     1141 acggtgtggg cccaccgggc gcgccgcctg tgcgtacctg cacccacccg gccgcatgac
     1201 ggacacgtcg ggcggcacac tgacaccccc acctggagct gcccgtgacc gatgccgcga
     1261 ccggcacccc gccgaagccg tccttgccga agccgtcctt gccgaagctg accacgccga
     1321 agctgaccac gatccattcg accgccgaga tccaggcgct catcccgcat cgctggccga
     1381 tcctgctggt cgaccggatc gtcgaatacg acgccgaggc aaagcggatc gtcgggatca
     1441 agggcgtggc cgcgaccgag tggttcttcc agggtcactt ccccggtctg cccgtcatgc
     1501 ccggcgtcat ccaggtcgag gcgctcgccc agacgatggc cgtctacgtc gcgaaacagc
     1561 cggggttcgg ggaccggatc ggcctgttcg ccggcatcga cgattgccgc ttcaagcgcg
     1621 tcgtcgtgcc cggcgatgtc ctccgcctcg agatcaccat ggagaagctc ggcagccggt
     1681 tcgggcgggc aaaggcggtc gcttcggtcg acggtgaggt ctcctgcgag gcgacgctca
     1741 gcttcgtcat ccccggcgaa ggcgtcctgc gatgacagcg cgggtcgcga tcatctccga
     1801 tgtccacggc aacctgctgg cgctgaccgc ggtccgggcc gccctcaaga aggaccgccc
     1861 cgaccacgtc gtcatcgccg gcgatcacgt gttccaggga ccggacccgg ccggcgtggt
     1921 cgacgccctc cgcgagatgg aggcgaacgg ggcgacgatc atccagggca acaccgatgt
     1981 cgccgtcgcg gacttcgact acggcgcggc gtttccgtgg ttcaccgacg gcgtgccgga
     2041 catggtccgc gacgcggccg agtgggccca cgacgcgctc ggcgacgagc gcgtgtcctg
     2101 gctgcgccgg ttgccgagtg agcgacgcct gatgctcgac gacacgatgc tcctcggttg
     2161 ccacgcctca cccggatcgc agacccaggg cttcgacatc cagctcgacc cgaacgtcat
     2221 cctcgagcgg atctcgagga cggacgcccg gctgatctgc tgtggccaca cccacctgcc
     2281 cgagatccgc gatctcggct ggaagatgat catcaacgcc ggctcggcgg gctacgtctt
     2341 cgacggcgac ccgaccgcgt cgtgggccct cgtcgaggtc gacgggaccg aggtccgggc
     2401 cgagatccgg cggacggagt tcgacacgat gatcgtcgcc aacgcgatct cggagcgcgg
     2461 cctggtcggc gacgtctacc gggcggccac ggtccggacg ggcaagctcg ttcgatgacg
     2521 gagcagcgcc gatgaccgag cagcgccgac cgaacccgga ctggccggcc gagccgcgcc
     2581 gggtcgtcgt gaccgggatg gggatgctga cggcgctcgg ccgggacgtc gccacgacct
     2641 gggccgggat ggtcgccggc cggtccggcg tcgccccgat caccctgttc gatcccgagc
     2701 gactcggctg ccggatcgcc ggcgaagtca aggacttcga cagctcccac gtcctcgaca
     2761 agaaggagat gcggcggacg gatcggtaca tccagctcgg cctcgtcgcc gcccgcgagg
     2821 cgatggacca ggcgggcctg cccgagcgga tggagggcga ggaggccgag cggaccgggg
     2881 tgatcttcgg gacggggctc ggcggcgtcg ggacgctcgt cgaggggatc tcgatcaacg
     2941 ccctccgcgg tccagaccgg atcagcccgt tcttcatccc gatggggatc ccgaacgtcg
     3001 gcgcggggat gatcgcgatc agcttcggga tgacgggccc gaacttcacg acggtgtccg
     3061 cctgcgcgac gggcggccac gcgatcggcg aggcctggga gacgatccgc cgggccgatg
     3121 cggacatgat ggtcgccggc ggggcggagg ccggcatccg cgagccgctc gtcggcgggt
     3181 tcgactcgat gcacgccctg tcccgacgga acgacgaccc ggaaggcgcg tcgcggccct
     3241 tcgaccaggg acgcgacggg ttcgtgcccg gcgaaggctc gggagcgctc gtcctcgagg
     3301 agctcgagca tgcccgggcc cgcggcgcga cgatctacgc cgagatcatc ggctacggcg
     3361 cgaccgccga cgcgtcgcac atcaccctgc cggccccggg tggcatcggc gccgtccgcg
     3421 ccgccgagcg agccatgcgc aaggcgggcg tcgtgccggc cgatgtcgtc cacgtcaacg
     3481 cccacgcgac ctcgaccccc gaaggcgacc gggcggagct ccaggcgatc cggacgacat
     3541 tcggggagca tgccggctcg gtcacggtca cgagcaacaa gtcgatgatc gggcacaccc
     3601 tcggcgcggc cggggcgatc gaggccatcg tcacgatcca gacgatccgc gagagctgcg
     3661 tcccgccgac gatcaacctc atcgatccgg acccggccgg cgagggcctc gatctcgtcg
     3721 ccaacgtggc gcgccggcag gacgtcccga tcgcgctgtc caactccttc ggcttcggcg
     3781 gccagaacac ggccctgatc atccgccggt ggaccgaatg accgagcacg acgaagaggt
     3841 cgacccgccg atcgagctcg accacgagca cgacgcggac gggaacgtcg tcccccggcc
     3901 cggcctgacg aacggcgacg gcgggggcgt cgatcccgcc ggggcagcgg cgcggccgga
     3961 cctcgacccg ggcgacgcgt cgctcctggc cctcgtcgac cggctcggat cgctgctcga
     4021 tcggagcgag ctgtcggagc tggcggtcga ggccggcggg acgcgtctcg tcctccgcac
     4081 gcccgccgca atcgcgacga cggtgatcgc cggcccggcc tggagcggcg agccggacgc
     4141 gggcgcggcc cttgcggcgt ccgcgaccgg gggaggctcg gacgcgtcga cggtcggatc
     4201 cgcacagacc ggtggtcgga ccgaggcacc ggcccgcccg agcgtcaagg cgccgctgac
     4261 cggtgtctgg tacagctcgc cggcacccgg ctcggcgccc ttcgtggcgg ttgggcgcga
     4321 ggtggccgtc ggccaggtga tcggcctgat cgaggcgatg aagctcttca acgagatcaa
     4381 gtccgatctt gccggacgag tggtcagggt ggtgcccgag agcggcgccc tggtcaaggc
     4441 gaagcagccg ctgatcgagg tagaaccgct gtgaacgacg cccgaccggc cggaaccgcg
     4501 gcctcgacca cgacccgcgc ccacgtgacc ggctggggcc gctacgcacc tgcccacgtc
     4561 ctgaccaacg ccgacctcga gaagctcgtc gacacgtccg acgagtggat ccagtcgcgg
     4621 accgggatcc gcgagcggcg ggtggcggcg gcccacgaga cgaccgcgtc gatggctgcc
     4681 gtggccgggc tccgggcgat ccggaccgcc ggcctcgagc ccgacgacat cgacctgatt
     4741 ctcctggcga cgttgacgcc ggactactgg atgccgtcga cggccgccct cgtcaaggag
     4801 gcgatcggca acacgaaagc caccgcgttc gacgtcatgg ctgcgtgcag cgggttcgtc
     4861 tatggcttcg cgaccgccca ggcctacatc caggccggtc tcgcgaagca cgttctcgtg
     4921 atcggcgcgg agctgctgac gcggttcctc gattacaccg accgaggcac ctgcatcctg
     4981 ttcggtgacg gcgccggcgc cgtcgtccta tcggcgtccg acgagcccgg cggcgcgctc
     5041 ggcgtcgaga tgacgaccga gccgcgtggc gcctacatga tctggctgcc ggccggcggg
     5101 tcgaaggcgc cgccgtcggc gggcacgatc tcgcgcggcg agcactacgt ccggatggaa
     5161 ggccgcgaga cctatcgctt cgcgacgaag acgatggcga cgaccgccct cgagtcgatc
     5221 cggcggtccg gcctcgagcc cggcgacatc gacctgttca tcccccacca ggcgaacgtc
     5281 cggatcatcg aggcggtggc caaaggtctc gacctgccga tggacaagat gttcatcaac
     5341 ctcgacaagt acggcaacac gtccgcggca tcggtcccga tcgccctcgc cgaggcggtc
     5401 aacgaaggcc gtgtcgcgat cggcgacaac gtcacgatcg tcgccttcgg ggcgggcttc
     5461 accagcggcg ccgtcacgat cgaatggacc gccgacccgg cccgcggccc gatcggcgat
     5521 gcggccgtgc ggccggagga cgtgaacgtc cggctaccgg tcgactggga ctcggtcgac
     5581 ccgatcccgg aggccctcgc cgagatcatg cgccggccgg gcccggtcga cgtcccgctc
     5641 gacgacgtcg tcccgggtga gccggagccc gctcaccggg aggtccacgc gtgatcgacc
     5701 tctcgggcaa gatcgccctg gtgacgggcg gatcgcgcgg gatcggcaag gcgatcgccc
     5761 tccggctcgc gacccagggc gccgacgtgg ccttcagcta tcgcggcaac gaggcggcgg
     5821 ccgcctcgac gaccgccgag atcgagggcc tgggccggag ggcgctggcg gtccaggcgg
     5881 acgtgtccga tgccgacgcc gcagccgggc tcgtcaaggc cgtcctcgag gccttcggca
     5941 aggtcgacat cctcgtcaac aacgccggca tcacccgcga cgacctgatc atgcggatga
     6001 gcgtcgaggc gttccgggag gtcctcgaga cgaacctctt cggcgcgttc tacatgacca
     6061 aggccgtgac ccggccgatg ctcaaggcca agggcgggcg gatcgtcaac atcacctcgg
     6121 tcagcggcca ggccggccag accggccagg cgaactactc cgcggcgaag gccggcctca
     6181 tcggcctgac caaggcgacg gcccgcgagc tcgcgtcgcg gacgatcacc tgcaatgccg
     6241 tcgcgcccgg gttcgtcctg accgagctca ccaaggacct gcccgaggcc ctccagtccc
     6301 agatcacggc ggccacgccc ctcgggcggt tcgggacgac cgacgagatc gccaacgctg
     6361 tcgccttcct cgccagcgac gaggctgcgt tcatcaccgg gcaggtgctt gccgtcgatg
     6421 gcggcctggt gatgatgtaa gtcgacgcct cgtcgttcga cccgccttca gggctggtga
     6481 tcaagcctcg cgcgccatgc gatcgcggat ccgatgccaa agaagcggcc acagcaatcg
     6541 gccgatcggg tcgcggatca ggccccgtgg cgtttccgcc tcgaccgaga cccgcgttcc
     6601 ccgcgaggcg tcgaccggcg caagccaata cctgaacgat ccgttcacgc cgcctgatcc
     6661 acgaccggtg gagctgatcg ccacagcaac ctcgtcgcgc tccagagcga tgtactcgac
     6721 acggacgcgc aactggtcga actgatggac tgaccaggat cctcgccgga ctggcggatc
     6781 ggactgttcg atcaccgggc cgacgtgatt ggcgatgagc aggtttctgg gtcgcgagag
     6841 atacgcgaca acccgatcgg gcggaaccgg aatgtcaaag ctggcagtgg tgtgcacgtg
     6901 ccgagactac gtgaccgacc gatccccgcg agcccgcgat cccgctcgaa gtcggtcccg
     6961 ccggaatgtc cgcaccacgg gcgttgcgac cgacaaggct cgactcgagc gattggctgg
     7021 cgtcgcgccg cgcgtggccg cgtgtggcca cgcgcgagcc gggcatcgca ttgcccgcac
     7081 cacggacatt ccgcatgttc gatcgagtga aataggcgta acgcccgcac gacggacatt
     7141 ccggaaccgt gaggtcgggc ccggtcagag cccctgccca atccgcggtg ctacgctcga
     7201 tccgatgcag attcgccagt tccgggtcga agggttgggt catctgtcga ccctcgttgc
     7261 cgacgagacg gccggggtgg ccgccgtcgt tgacccgcgc cgtgacgtcg attgctacct
     7321 cgccgcggcc cgcgagctcg acgtccggat cacccacgtc gtcgagaccc atctccacaa
     7381 cgactacgtc tcgggcggtc gcgacctggc ggcgctgacc ggatcgcggc acgtcatcgg
     7441 cgccggcgcc gagctccgcc acgagcatgg cccggtccgg gacggcgaga ccttcgacgt
     7501 cgggtcgctg cggttcacca gccacgacac gccgggccac acccccgagc acgtcgccta
     7561 ctcggtcgcc gaccttgacc gaggcgagga ccccgcgctc ctgttcagtg gcggctcgct
     7621 gctcgtcggc gcggtcgggc ggaccgatct cctcggcgag gagcacgctc gggactacgc
     7681 ccgggcgatg caccgctcgc tccacgaggt cgtgctcgcc cacgacgacc acgtcgccgt
     7741 ccacccgacc cacggtgcgg gctccctctg cgcgacgggc atcgccagca ccccgacgac
     7801 gacgatcggc tacgagcggc gccacaacgc gctgctgcgg gtcacggacg tcgaggcgtt
     7861 cgcgacgcgg ctgctgcgcg gccagccggc ggtcccgcac tacttcgccc ggatgcgacc
     7921 gaccaaccag gcgggcccgg tgctgctggg cgggcgcgtc ccggagccgc ggccgctcag
     7981 cctcgacggg gcgcggagcg cactcgcagc cggtgccctt ctcctcgacc tccgcccgcc
     8041 cgccgaccac gccgtctcgc acgtccccgg ctcgctgtcg atcccgctcg ggccgtcctt
     8101 cgggacgtgg ctcggctggg tcgtcgagcc cgctcgtccg ctcgtcctga tcctcgagcg
     8161 gaccgacgac tgggacgagg ctgtccgcca ggccctccgg atcggcgccg agggatcgat
     8221 cgtcggacac ctccgcggcg gcctcgggac gtggtcggat gccggcgcac cactcgaatc
     8281 gaacggccgg ctcagcgtgg atgagctggc cgcgacgatc gagcgtgcgg cgccggccga
     8341 cgcgccgctc gtcatcgacg tccgccaggc caacgaatac gagaccgggc acgtccccgg
     8401 cagcatccac atcgccgcgg gctcgctccc ggaccgcctt gccgagctgc cgcgacagcg
     8461 gccgatcgcg acgatctgcg cgtcgggtct gcgctcgagc atcgccgctt cgatcctccg
     8521 atcggccggg ttcgacgacg tctcgtgggt cgcgagtggc gtttccgcct ggcgtgccgc
     8581 cggccacccg acggtccgcg gcgatcagcc gacccgtccc acggcgacga gccggccggg
     8641 cgcggccggg accggcgccc gcgccggcag ggacgcaggg acggtcgccg cgcacgactg
     8701 acgcggatca cagggaccat gtcggtcagt cgcggccacg ccccccggtc ggggcggtac
     8761 gttcgccctc gatcgggggt acctcgggcc ggaagaggcg cccgcgcgaa cgtgggatgg
     8821 tcgacccggc catgagcgcg catccgccca ccagaccgcg gcgtcgcgac acgcgcgctt
     8881 ccggacgtcc ctccggggcg cggtcgcttg cggcgcccgg cattgcgccg gcgggcacat
     8941 cgaccgtcac gccggccctc gcgtcaccgg tccgggcgac cgacgaatcg atcgcggcgc
     9001 tcttcgcccg ccagctcgag gtgctccagg cggcctcggc gcgtctcagt cgagccgagg
     9061 gcagcgaggc cgtcgggcgg gcggtcgtcg aggaaaccgg ccggatcatc gattaccaca
     9121 acgcgcgcgt ctacgtcctc gaagcgggcg gcgacctcgt cccgatcgcc ttcgaaggcc
     9181 gggtcggcga atacgagaag gtcgacctcg agctcctccg gacgcgactc ggggtcggct
     9241 tcaccggctg ggtcgggctg cacggcgagc cgcttctcgt acgtgacgcg aacgacgacc
     9301 cgcgcggtgc gtcgatcccc ggcaccgacg acgtcgacga atcgatgctc gtcgtgccca
     9361 tgcgttacga cgacgccgtg gtcggcgtga tcaccctctc gaagctcggc ctcgaccagt
     9421 tcgacggtca cgacctgcgc ctcctgtcga tcctcgccga ccaggcggcg accgccctcg
     9481 aatccgctcg caacctcgcc cgcagcgacg cgctcgcggg cgagctgcgg ctgctcctcg
     9541 acgtgagcag ccagcttgcg gtcagcctcg actcgcacca ggtcgcaaac atcctcgccc
     9601 gccatctcgc gaccgtgatg gacgtcgagg aatgcgcgat ctcgtggtgg gaccggcccc
     9661 acgaccgagt cgccagcatc ggctactacc cgcccgtgcc ggtcgcggag ctcacgccgt
     9721 tcttcgacat ctcggactac ccgtcgacca agcacgtgct cgaggaccag g
//
LOCUS       DATAIW010000335         5347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_170637, whole genome shotgun sequence.
ACCESSION   DATAIW010000335 DATAIW010000000
VERSION     DATAIW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170637"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VHM48_10315"
     CDS             <1..444
                     /locus_tag="VHM48_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2755848.1"
                     /translation="ARRAGQPAATRSLPVALLVALILYVNEIPDRRGDARVGKRTLPV
                     RFSKAAVIGGYRGAAVAAYVIVVAGVVAGLLPIPTLLALLTIPLAVQVSRGLEPNYDN
                     PYGLMAIMGVNVKVHLYAGLLLLAGYAVVLILGAVAPSVSLFVGR"
     gene            complement(532..1881)
                     /locus_tag="VHM48_10320"
     CDS             complement(532..1881)
                     /locus_tag="VHM48_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2755849.1"
                     /translation="MPRRSLALLATVTLLLGLAAPAAQAKTAAAIDAAGASALCESLF
                     SGTYDVSSSEPLAACQWDMSLINANDATHARATGKGVRVGDIDSGVDLTHPDIVPNLD
                     LGDSCSFIFDHDPTALPQEMANGDCSNKAAVQDYSGHGTHTASTIAAPINGIGIAGVA
                     PEATIVAIKACTWVGYCFADSVAAALRYAGDLRLDVVNMSLFADPYLYYCGSDAEQRA
                     LYKSLQSAARYAQQRGVLIIAAAGNQADDMTHPTTDIISPDWPPDSAVERVVHNNCKR
                     APTELPGVLAISATGPIGIPGYTLNITTYSDVGMGVVGAAAPGGDYFIATGTVQDAIL
                     AAWTSTDDGTFDFYSTLTQYPGLVVLDHGARYIEINGTSMAAPHATGVAALIKQLHPR
                     WGPAAVSAALQRTASPLACPADWQPLYPEDLRLRCYGGGGRTSFFGNGLVDAWAASH"
     gene            2341..3081
                     /locus_tag="VHM48_10325"
     CDS             2341..3081
                     /locus_tag="VHM48_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755850.1"
                     /translation="MDTVAFGRGFRALRLRKRLRQDDLAAAAGVSRGAIARIEQGHAD
                     KVTIETLEQVAKPLGARVVCRLSWNGEYLDRLLDADHAAIVEQVVRFLAAAGWLVATE
                     VSFNVWGERGSIDVLAFRPDTRILLVIEVKSVVPDVQATLVTLDRKERLAPEIARERG
                     WDAVAVARLLVIRESRTTRRRIEEHTATFGNAFPDRARTIRTWLARPEAGRPLRGLWF
                     LSNESQAVAMQRVRRRPRLPERGPEPGP"
     gene            complement(3296..5263)
                     /gene="dnaK"
                     /locus_tag="VHM48_10330"
     CDS             complement(3296..5263)
                     /gene="dnaK"
                     /locus_tag="VHM48_10330"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX2755851.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPTVIASAEGGRTVPSVVAFTK
                     TGERLVGQLAKRQAVTNPANTIYSIKRFMGRRWDDSETKRAKELVSYTVEKDAKSDGV
                     VVKVTDKTYTPPEISAMILQKLKADAEAYLGEKITEAVITVPAYFDDTQRQATKDAGR
                     IAGLEVRRIINEPTASALAYGLDKKHDEKIAVYDLGGGTFDISILELGEGVFEVKATN
                     GDTHLGGDDFDQRVIEWLLAEFKKDQGIDLAKDPQALQRLKEAAEKAKIELSSTSSTE
                     INLPYVTADASGPKHLVVTLTRAKLEDLVRDLVEKTRKPVEAALKDSGLKAGDIDEVI
                     LVGGMTRMPAVVEAVKAMFGGKEPHKGVNPDEVVGIGAAIQAGVLAGDVKDVLLLDVT
                     PLSLGIETLGGVMTKLIDRNTTIPTSKSQVFSTASDNQGQVEIHVLQGEREFAADNKT
                     LGRFILDGIPPAPRGVPQVEVTFDIDANGILDVKAKDRATSKEQQVRITASSMLSKDD
                     VDKMVREASEHADEDKQRREEVETRNQAEQLTYQAERTIKDLGDKVSSEDKVTVENQV
                     TSLREALKGSDMSAITAGATALAETLNKVSTAAYQAAAAEASANGSSSEGAEPGTEGA
                     EPEGEKAGAGARSGGPADSSDDTVEGEFKEV"
BASE COUNT          711 a         1783 c         1934 g          919 t
ORIGIN      
        1 gcgcgacgag cagggcaacc ggcagcgacg cgatcgctgc cggttgccct gctcgtcgcg
       61 ctgatcctgt acgtcaacga gatccccgat cgccggggcg acgcgcgcgt cggcaagcgg
      121 accctcccgg tccgcttctc gaaggcggcg gtcatcggcg ggtatcgtgg cgccgccgtc
      181 gcggcgtacg tgatcgtcgt cgcgggcgtc gtcgccgggc tcctgccgat cccgacgctg
      241 ctcgccctgc tcaccatccc gctcgccgtc caggtgtcgc gcggcctcga gccgaactac
      301 gacaacccgt acggcctgat ggcgatcatg ggcgtcaacg tgaaggtcca cctgtacgcc
      361 ggcctgctcc tgctggccgg gtacgccgtg gtcctgatcc tgggcgcggt ggcgccgtcg
      421 gtgagcctct tcgtcggtcg gtagcggtcc gcggtcgcgg acgacccgaa atctgaagcg
      481 cccggccgtc gatcggccgg gcgcttcgtc gcatgctgag cgagcgatgg gtcagtggct
      541 ggcggcccag gcgtccacca gtccgttgcc gaagaacgag gtcctgccgc caccgccata
      601 acagcggagc cgcaggtcct ccgggtacag cggctgccag tcggccgggc aggccagtgg
      661 cgaggccgtc cgctggaggg cggcggagac ggccgccggc ccccagcgcg gatggagctg
      721 cttgatcaac gccgcgacgc cggtggcatg cggggccgcc atcgaggtcc cgttgatctc
      781 gatgtagcgc gctccgtggt cgagcacgac gaggcccggg tactgcgtga gggtggagta
      841 gaagtcgaag gttccatcgt cggtcgaggt ccaggccgcg aggatcgcat cctggacggt
      901 cccggtcgcg atgaaatagt cgcctccggg cgccgcggca ccgacgacgc ccatcccgac
      961 gtcggagtac gtggtgatgt tcagggtgta gccggggatg ccgatcgggc ccgtcgcgga
     1021 gatcgcgagg acgccgggca gctcggtggg ggcgcgcttg cagttgttgt ggacgacccg
     1081 ctcgaccgcg gaatccggcg gccagtccgg cgagatgatg tcggtcgtcg ggtgggtcat
     1141 gtcgtcggcc tggttgccgg ccgcggcgat gatcaggacg ccgcgctgct gggcgtacct
     1201 ggcggccgac tggaggctct tgtacagggc ccgctgctcg gcgtcgctgc cgcagtaata
     1261 caggtagggg tcggcgaaca ggctcatgtt gacgacgtcc aggcggaggt cgcctgcgta
     1321 gcggagcgcg gccgcgacgg agtcggcgaa gcagtagccg acccacgtgc aggccttgat
     1381 cgcgacgatc gtcgcctcgg gggcgacgcc ggcgatcccg atgccgttga tcggcgcggc
     1441 gatcgtcgag gccgtgtggg tgccatggcc ggaatagtcc tggacggcag ccttgttcga
     1501 gcagtcgccg ttggccatct cctggggcag cgccgtcggg tcgtggtcga agatgaacga
     1561 gcacgagtcg ccgagatcga ggttcgggac gatgtcgggg tgggtcaggt cgacgccgct
     1621 gtcgatgtcg ccgacacgga cgcccttgcc ggtcgcccgg gcgtgggtcg cgtcgttcgc
     1681 attgatcagc gacatgtccc actggcaggc ggcgagcggc tcgctcgacg agacgtcgta
     1741 cgtgccgctg aacagcgatt cgcacagcgc cgacgcgccg gcggcatcga tggcggcggc
     1801 ggtcttcgcc tgggctgcgg gcgcggccaa cccgagcagg agcgtgacag tcgccaggag
     1861 cgcgagggat cgacggggca cggtcgtctc ctccgggccg attcggccca cgccggctcg
     1921 cgcgcggccc gcggcggctg ccggcacctc ctcatgcgag ggcgggcctg agacgcggat
     1981 cgttgcgcgt cggcaccggt ctggcaagcc caagggcacg tccgcggtgc gggcgagcac
     2041 catcgttcgg cccggccggt gtggcgatca gtcatccgta actgagaacc tccccggtac
     2101 cggcgacgca gaacgccgtc cgttccaggc cgtactgatc gttcgagatc ggagcgggat
     2161 cgaagccccg cgcctttggc agccggaacc cgatcgacat gtcaatcgag ccacttcgac
     2221 cgatccccat cccgtccaac cggaagcagc ccacgggcgc gtcggcggct cggtacggcg
     2281 ggagaccgac ctgccctctg cagtaagtcg ccgcgaatgg cgcacggcca tcatccacca
     2341 atggacaccg tcgccttcgg tcgcggcttc agagccctgc gactccgcaa gcggcttcgg
     2401 caggacgacc tcgcggccgc agccggcgtc tcccgcgggg ccatcgcccg gatcgagcag
     2461 ggccatgctg acaaggtcac gatcgagaca ctggagcagg ttgccaagcc gctgggcgcc
     2521 cgagtggtct gccgcctttc gtggaatggc gagtacctgg atcggttgct cgacgcggac
     2581 cacgccgcga tcgtcgaaca ggtcgtgcgc tttctggcag ccgcgggttg gctcgtcgcg
     2641 accgaggtgt cgttcaacgt ctggggcgaa cggggatcga tcgacgtcct cgccttccgt
     2701 cccgacaccc ggatcctcct cgtcatcgag gtcaagtcgg tcgtgccgga cgtgcaggcg
     2761 acgctggtga cgctggatcg caaggagcgc ctcgctccgg agattgctcg tgagcgcggg
     2821 tgggatgcgg tcgccgttgc tcgactgctg gtcatccgag agagccggac gacgcggcgg
     2881 cggatcgagg aacacacggc cacgttcggc aacgcgtttc cggatcgagc ccgcacaatc
     2941 cgcacctggc tggcgcgacc ggaggctgga cgaccgctcc gcgggctgtg gttcttgtca
     3001 aacgaatctc aggcggtcgc aatgcagcgg gtacggcggc gtccgcgtct ccctgagcgt
     3061 ggcccggagc cgggtccctg acaggcgaat ctcaggcggt cgcatgacac gtcggcgccg
     3121 cctaccgacc gggtgggatt cgcatgacaa gtcggcccgg cgggatcggc cgccgacggt
     3181 cgcccggggc cggaggatgt ggatcggccg ccgcggcacg ggcagaatcg aagcgcccga
     3241 ccggggagcc cggccgggcg cgacgttctt gcggtcagcg gcgagtcgcc gcggttcaga
     3301 cctccttgaa ctcgccctcg accgtgtcgt cggaggagtc cgccgggccg ccggagcgag
     3361 ccccggcgcc ggccttctcg ccctcgggct ccgcgccctc ggtgccgggc tcggcgcctt
     3421 cggacgacga cccgttggcc gaggcctcgg cggccgccgc ctggtaggcc gcggtcgaga
     3481 ccttgttgag cgtctcggcg agggccgtgg ctccggccgt aatcgcgctc atgtcggagc
     3541 ccttgagggc ctcgcgcagc gaggtcacct ggttctcgac ggtcaccttg tcctcggacg
     3601 acaccttgtc gccgaggtcc ttgatcgtcc gctcggcctg gtaggtcagc tgctccgcct
     3661 ggttgcgggt ctcgacctcc tcgcgccgct gcttgtcctc gtcggcgtgc tcggacgcct
     3721 cgcggaccat cttgtcgacg tcgtccttcg agagcatcga ggacgcggtg atccggacct
     3781 gctgctcctt gctggtcgcg cggtccttgg ccttgacgtc gaggatgccg ttcgcgtcga
     3841 tgtcgaacgt gacctcgacc tggggcacgc cgcgcggcgc cggcgggatc ccgtcgagga
     3901 tgaaccggcc gagcgtcttg ttgtccgcgg cgaactcgcg ctcgccctgg aggacgtgaa
     3961 tctcgacctg gccctggttg tccgaggccg tcgagaacac ctgcgacttg ctcgtcggga
     4021 tcgtcgtgtt gcggtcgatg agcttggtca tcacgccgcc gagcgtctcg atcccgagcg
     4081 acagcggcgt cacgtcgagg agcaggacat ccttgacgtc accggccagg acgccggcct
     4141 ggatcgcggc gccgatgccg acgacctcgt ccggattcac gcccttgtgc ggctccttgc
     4201 cgccgaacat cgccttgacc gcctcgacga ccgccggcat ccgggtcatg ccgccgacga
     4261 ggatgacctc gtcgatgtcg ccggccttga gcccggagtc cttgagcgcg gcctcgaccg
     4321 gcttgcgggt cttctcgacg aggtcgcgga cgaggtcctc gagcttcgct cgagtcaggg
     4381 tcacgacgag gtgcttcggg cccgatgcgt cggccgtgac gtagggcagg ttgatctcgg
     4441 tcgaggaggt cgagctcaac tcgatcttcg ccttctcggc ggcctccttc aggcgctgga
     4501 gggcctgggg gtccttcgcc aaatcgatcc cctggtcctt cttgaactcg gccagcagcc
     4561 actcgatgac gcgctggtcg aagtcgtcgc cgcccaggtg ggtgtcgccg ttggtcgcct
     4621 tgacctcgaa gacgccttcg cccagctcga ggatcgagat gtcgaaggtg ccgccgccga
     4681 ggtcgtacac cgcgatcttc tcgtcgtgct tcttgtcgag gccgtaggcc agcgccgacg
     4741 cggtcggctc gttgatgatc cggcggacct ccaggccggc gatccgcccg gcgtccttgg
     4801 tcgcctggcg ctgggtgtcg tcgaagtacg cggggacggt gatgaccgcc tcggtgatct
     4861 tctcgccgag gtaggcctcc gcgtccgcct tcagcttctg gaggatcatc gcgctgatct
     4921 cgggcggcgt gtaggtcttg tcggtgacct tgacgacgac gccgtcgctc ttggcgtcct
     4981 tctcgaccgt gtagctgacg agctccttcg cccgcttggt ctcgctgtcg tcccagcggc
     5041 ggcccatgaa gcgcttgatc gagtagatcg tgttggcggg gttcgtgacc gcctggcgct
     5101 tggccagctg gccgacgagg cgctcgccgg tcttggtgaa ggcgacgacc gacggaacgg
     5161 tccgaccgcc ctctgccgat gcgatgacgg tcggctcgcc gccctccatg acggcgacga
     5221 cgctgttcgt cgtgccgagg tcgatgccga tgatcttgcc catggggttc gtgtctcctg
     5281 tcggtgggtt cggggtcagt gggtggatga gtgcaggtgg ttggcgggat cagttggggc
     5341 gggcggc
//
LOCUS       DATAIW010000336         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_110976, whole genome shotgun sequence.
ACCESSION   DATAIW010000336 DATAIW010000000
VERSION     DATAIW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110976"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="hisS"
                     /locus_tag="VHM48_10335"
     CDS             complement(<1..800)
                     /gene="hisS"
                     /locus_tag="VHM48_10335"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX2755852.1"
                     /translation="MPTFRAPRGTRDVLPAERAAFIRLERIGADLAARYGYRPIETPL
                     FEQTAVFERGVGAVTDIVEKELFRIAPRTEDGETWALRPEPTAGIVRAYVQHGMQTLP
                     QPVKLTLTGPMFRYDRPQAGRYREFWQFDIEAIGDPGPAVDAEIIELADRFYREAGIR
                     EPEILLNSIGDRDCRPAYVAELAAYYGGHLERLPSLERERLERSPLRLLDSKEPVMVE
                     LNAGAPSITDHLCGPCAEHFAGVRAHLDAVGVAYRLEPRLVRGLDYYTR"
     gene            1063..1326
                     /locus_tag="VHM48_10340"
     CDS             1063..1326
                     /locus_tag="VHM48_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755853.1"
                     /translation="MRDSYLTSADDRRGVLSIADAIRYAETVMAAIELDIDPWLAQLD
                     EAFGNDAIHGRWGPLPGHRSASDRLPLPTRSKGVLGADEADEA"
     gene            1372..2118
                     /locus_tag="VHM48_10345"
     CDS             1372..2118
                     /locus_tag="VHM48_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2755854.1"
                     /translation="MGPHGVSVRVLAGYLAGIALGTMSITLLFLGMRAVMDVGGACAD
                     GGPYVSAQPCPTGVPLAMVGGMFGLFGSAGLTVWFGSRIGKAAASIVALGWPALFLSL
                     GLNFLDYAFHPPDNEATPVWGWLIPGVLFWLMGGAPLAVGILAWREARAGRPVNRLSN
                     QVAASIPDRPIVFGTRFTAPRPPEPARVEFAPGSWGATRPTPSVEPPTEPSDLVDDLT
                     RLAELHEAGDLSDEEFATAKRDRLGQETRG"
     gene            complement(2467..>3864)
                     /locus_tag="VHM48_10350"
     CDS             complement(2467..>3864)
                     /locus_tag="VHM48_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX2755855.1"
                     /translation="KSAEFAEIYDVYAIRILVDDVRDCYAALGTVHALWRPIPGQFDD
                     YIAVPKNNRYQSLHTAVIALDGKPLEIQIRTHAMHQVSEVGIAAHWRYKDGTKSDREY
                     DAKLAWLRQLMEWQRDVSESDATEFVEGIKLDIFQDQVFVFTPKGDIKDLPAGATPLD
                     FAYRIHTDVGHRTIGAKVNNRLVPLDYRLKNGDIVEIVTTKGEHGPSRDWMNVVRTSH
                     AREKIRQWFKRKDRDENIIHGRESLERELRRLARKSLQAVGNDKIAEVGRTIYNHDTL
                     DDFYAAIGYGAVSPQQVVGRLGVLDDTEVGLPTVAPPNLPARTGGVRVKGVGDLLVRF
                     AKCCHPIPGDPIVGFITRGKGVTVHLRSCPTVLNEREVPRLIEVEWEAAPAQTYPIAI
                     RVEAYDRTGLLNDITQVVAENKVNIVAAAVHTNPDHSALVTATLQVASVSQLARVMSR
                     IEGLKDVINVQRDLN"
BASE COUNT          526 a         1319 c         1385 g          634 t
ORIGIN      
        1 cgcgtgtagt agtcgaggcc gcggaccagt cgcggttcga gacgataggc gaccccgacg
       61 gcgtcgagat gcgcccggac gccggcgaaa tgctcggcgc acggcccgca gaggtggtcg
      121 gtgatcgacg gcgccccggc attcaactcg accatgaccg gctccttcga gtcgagcagg
      181 cggaggggac tgcgctccag ccgttcgcgc tcgagcgacg gcagccgctc gaggtggccg
      241 ccgtagtagg cggcgagctc ggcgacgtag gcgggccggc agtcccggtc gccgatcgag
      301 ttcaggagga tctcgggttc gcggatcccg gcctcgcggt agaagcggtc ggcgagctcg
      361 atgatctcgg cgtcgaccgc cggtccgggg tcgccgatgg cctcgatgtc gaactgccag
      421 aactcgcggt agcgtccggc ctgcggacga tcgtagcgga acatcggccc ggtcagagtc
      481 agcttcacgg gctgcggcag cgtctgcatc ccgtgctgga cgtaggcccg gacgatgccg
      541 gcggtcggct ccgggcgaag ggcccaggtc tcgccgtcct cggtccgcgg cgcgatccgg
      601 aagagctcct tctcgacgat gtcggtcacg gcgccgacgc cccgctcgaa gaccgcggtc
      661 tgttcaaaga gcggcgtctc gatcgggcga tagccgtagc gcgcggcaag atccgcgccg
      721 atccgctcga gccggatgaa cgcagctcgc tcggcgggca ggacgtcgcg ggtgccccgc
      781 ggggcgcgga aggtcggcat cgttcgcgaa gtctagcggg cgcccatcgg acccgaccgg
      841 ccgacgtatt cgggcagccg acgggcggtc ggggtgactg ggtccgtcgt tcggacgagg
      901 tccgtgcggg accaccgccg atggcgcacc accggtcctg cggatatcgc cgggcgagct
      961 ctgcggacat ggttgggctg tccggccaac caggccggcc tggaggcgtg aacgaacatg
     1021 ggctggactg agcgccaagc cttcttcgaa cggatcgtcg caatgcgtga cagctatctg
     1081 acgtccgccg acgaccgccg tggcgtcctg tcgatcgcgg acgcgatccg ctatgccgag
     1141 acggtcatgg cggcgatcga gctcgacatc gatccgtggc tcgcccagct ggacgaggcg
     1201 ttcggcaacg acgcaatcca tggccgctgg ggcccgttgc ccgggcatcg ctcggcatct
     1261 gatcgattgc cattgccgac tcgctccaag ggtgtcctcg gcgcggacga ggccgacgaa
     1321 gcctgacccg ccagggcacg cccaccctca ccggagccgt atgatccggg cgtgggtcca
     1381 cacggggtgt ccgtccgcgt cctcgccggc tatctcgccg ggatcgcgtt gggcacgatg
     1441 tcgatcacgc tgctcttcct cgggatgcgc gccgtcatgg atgtcggggg cgcctgcgcc
     1501 gacggcggcc cgtacgtcag cgcccagcct tgcccgactg gtgtcccgct cgcgatggtc
     1561 ggagggatgt tcgggctgtt cgggtcggcc gggctcaccg tctggttcgg gtcgcggatc
     1621 gggaaggccg ccgcgtcgat cgttgccctc ggctggccgg cgctcttcct ctcgctcggg
     1681 ctgaacttcc tcgattacgc cttccacccg cccgacaacg aggccacgcc ggtgtggggc
     1741 tggctcatcc ccggtgtcct cttctggctg atgggcgggg cgccgctggc cgtcgggatc
     1801 ctggcgtggc gggaggcccg cgcgggcagg cccgtcaacc ggctctccaa ccaggtcgcc
     1861 gcgagcatcc ccgaccggcc gatcgtgttc ggcacacgct tcacggcgcc acggccgccc
     1921 gagcctgccc gggtcgagtt cgcgccaggt tcatggggcg caacgcggcc gacaccgtcc
     1981 gtcgagccac cgaccgaacc gtccgacctc gtcgacgacc tcacccgtct cgccgaactc
     2041 cacgaggcgg gcgacctcag cgacgaagag ttcgcgaccg ccaagcgcga ccgcctcggg
     2101 caggagacac gaggatgagc gagcgcgtcg gtcgcgccct gcccctgctg atcgtcgggg
     2161 cggcgctcat cggggtctgg ctcggcgccg caatcttcgc ctcgatcagc taggcctcgt
     2221 cgccgcacgc cgcgcccgcg gccgctcgct gcgcacccgg cgcgcactac gacgaaggag
     2281 tgcctcgcaa accacctggg acggccgttc cgacgtcatt ctgcccgccc actgagcatc
     2341 gcagcggctg aaaccggtcc cgttcgtcgc aacgcccgcc cagtgtgcag tcgtgctccg
     2401 ggcggcgcgc cccgcgcgcc cggcgcgcgc gcaagtgccg gctcgtggcg agcgggcttc
     2461 gtcgcatcag ttcaagtccc gctggacgtt gatcacgtcc ttcaatccct cgatccggct
     2521 catcacccgg gccagctggg agaccgaggc aacctggagg gtcgcggtca cgagcgcact
     2581 gtggtccggg ttcgtgtgga ccgccgcggc gacgatgttg accttgttct cggccacgac
     2641 ctgggtgatg tcgttgagga gcccggtgcg gtcgtaggcc tcgacccgga tcgcgatcgg
     2701 gtaggtctgg gccggcgcgg cctcccattc gacctcgatc aggcgcggca cctcgcgctc
     2761 gttgaggacc gtcggacacg accggaggtg gaccgtcacg cccttgccgc gggtgatgaa
     2821 cccgacgatc gggtcgcccg ggatcgggtg acagcatttc gcgaaccgga cgagcaggtc
     2881 gccgacgccc ttgacccgga cgccgcccgt ccgcgccggg aggttcggcg gcgccacggt
     2941 cggcagcccg acctcggtgt cgtcgaggac gccgagccgg ccgacgacct gctggggact
     3001 gaccgccccg tagccgatcg ccgcatagaa gtcgtcgagg gtgtcgtggt tgtagatcgt
     3061 ccggccgacc tcggcgatct tgtcgttgcc gaccgcctgg aggctcttgc gagccaggcg
     3121 ccggagctcg cgctcgagcg actcgcggcc gtggatgatg ttctcgtcgc ggtccttgcg
     3181 cttgaaccac tggcggatct tctcccgggc gtgggaggtc cggacgacgt tcatccagtc
     3241 gcgtgacggg ccgtgctcgc ccttcgtcgt gacgatctcg acgatgtcgc cgttcttgag
     3301 ccggtagtcg agcgggacga ggcggttgtt gaccttcgcc ccgatcgtcc ggtggccgac
     3361 gtcggtatgg atccggtagg cgaagtcgag cggggtcgcc cccgccggca ggtccttgat
     3421 gtcgcccttc ggggtgaaca cgaagacctg gtcctggaag atgtcgagct tgatcccctc
     3481 gacgaactcg gtcgcgtccg actcgctgac gtcgcgctgc cactccatga gctgacgcag
     3541 ccaggcgagc ttggcgtcgt actcgcggtc ggacttggta ccgtccttgt agcgccagtg
     3601 ggcggcgatc ccgacctcgc tgacctggtg catcgcatgg gtccggatct ggatctcgag
     3661 cggcttgccg tcgagggcga tgaccgcggt gtggagcgac tggtagcggt tgttcttggg
     3721 gaccgcgatg tagtcgtcga actggccggg gatcgggcgc cacagggcgt ggacggtgcc
     3781 gagcgcggcg tagcagtcgc ggacgtcgtc gacgaggatc cggatcgcgt agacgtcgta
     3841 gatctcggcg aactcggccg attt
//
LOCUS       DATAIW010000337         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_51473, whole genome shotgun sequence.
ACCESSION   DATAIW010000337 DATAIW010000000
VERSION     DATAIW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51473"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VHM48_10355"
     CDS             complement(<1..231)
                     /locus_tag="VHM48_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX2755856.1"
                     /translation="MPNAAAVYARISSDPDGDRLGVTRQVEDCTGFAERRGWPVREVY
                     IDDDRSAYSGRVRPEYRRMLDDIRSGDVDAVIV"
     gene            complement(305..721)
                     /locus_tag="VHM48_10360"
     CDS             complement(305..721)
                     /locus_tag="VHM48_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2755857.1"
                     /translation="MSTNVVDALAHVPMLSGLDRKHLERLAKDFTERTFPAGAVIVRQ
                     GDDRGIGFFVIADGEAIVTVDGAEVSRLGPGTHFGAIALIADRVRTATVTAGTDLHCY
                     VMTLWDFRSFVQGDAEVAWKLLEQLAQMVQKASAQH"
     gene            863..1102
                     /locus_tag="VHM48_10365"
     CDS             863..1102
                     /locus_tag="VHM48_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755858.1"
                     /translation="MADPSEPTSHIEHYANGRLKMTGFHLAGEMHGDWEWYRLDGSLM
                     RTGSFDRGRQSGPWRTFDRAGRMVKETDFGEAAKG"
     gene            1249..1857
                     /locus_tag="VHM48_10370"
     CDS             1249..1857
                     /locus_tag="VHM48_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755859.1"
                     /translation="MNEPNDVWSPAGATTDDLAAPAAPLRTGRRATGRQKAKLAGALI
                     LGGVAGVAILGPLSVLAASPSPAASSGATTGTTGSGGSTGSGISGAAGTNDQRGPGGP
                     GGFGRNEAVSDESVVAKAIGISEADLKTALAGGQTVAAVAKAHSVDIQVVIDALVADG
                     QSELDAAVKAGTLTQAQADAEKANLTQRATDQANGTFGGGRH"
     gene            2005..3591
                     /locus_tag="VHM48_10375"
     CDS             2005..3591
                     /locus_tag="VHM48_10375"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX2755860.1"
                     /translation="MFVPLSVLEFRDRAALFFGDKVGIIDGERQFTYRAFAERTHRLA
                     NALRGIGVGAGDRVSFITYNTHQLLEAYYGVLEAGAVLNPINIRLAAHEIAFILDHAA
                     SRVVVFHHDFLPLIEQIGPRLASRPTFVVLEGDAGGLVDHEYEALLAGGSADPLHPEI
                     DENGLAELFYTSGTTGMPKGVAMTHRELYLHSLVAEVGLGFDQDDVVLHVVPLFHVNG
                     WGTPHFVTMIGGRHVMLRRFDPGALMELVERHRVTRLLAVPTIFNAILNHRERSSRDL
                     TSLRQVIIGGAPASPALVASLEAELGVQAIVGYGLTETSPIITLAQPRSFLTEHEPAE
                     RRQERQSMTGWAIPGVAVRVVDGDGHDVRPDGEQIGEIVVRSNLVMDGYYRDPDATAK
                     AIRGGWLHTGDMATIDEAGYVLIKDRSKDIIISAGENISSVEIEVALGAHPAVLECAV
                     IAAPDDKRGEVPVAFVVLKPDQTVSASDLRAFCREGLAGFKVPRQIQFRDALPKGGTG
                     KILKAELREPFWVGRSSRVN"
     gene            complement(3664..3879)
                     /locus_tag="VHM48_10380"
     CDS             complement(3664..3879)
                     /locus_tag="VHM48_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755861.1"
                     /translation="MAQLKIDAINPVRTDTWTTFLWVARMAPWELTCGFCRTRFTSIG
                     VFFRSSAVCPACGTRNLLPNSSRPRRG"
     gene            complement(3883..>4121)
                     /locus_tag="VHM48_10385"
     CDS             complement(3883..>4121)
                     /locus_tag="VHM48_10385"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2755862.1"
                     /translation="FFGSAHTLERFETAFYRPILSDWRNYETWLDDGAQTATQRANRI
                     WKQLLAEFEPPPLDPAVAEAIAAFVARRKRQISG"
BASE COUNT          658 a         1455 c         1385 g          623 t
ORIGIN      
        1 gacgatgacg gcgtcgacgt cgcccgatcg gatgtcgtcg agcatccgcc ggtattcggg
       61 ccggaccctc ccggagtagg cgctccggtc gtcgtcgatg tagacctcgc ggaccggcca
      121 gccgcggcgc tcggcgaacc cggtgcagtc ctcgacctgg cgggtgacgc cgaggcggtc
      181 accgtccggg tcggacgaga tgcgggcata gacggcagcg gcattcggca ccgggataca
      241 ttaacacacg ctattgtcgc aatgcgtgct gtcacacggc ggttacgccg ggacgagagc
      301 cgcgtcagtg ctgggcggat gccttctgga ccatctgggc gagctgctcg agcagcttcc
      361 aggcgacctc cgcgtcgccc tggacgaacg atcggaagtc ccacagggtc atgacgtagc
      421 agtggaggtc ggttcccgcg gtgacggtcg cggtccggac acggtcggcg atcagcgcga
      481 tcgcgccgaa atgggtgccg ggacccagac gcgagacctc ggccccgtcg accgtgacga
      541 tcgcctcgcc gtcggcgatg acgaagaacc cgatgccgcg atcgtcgccc tgccggacga
      601 tgaccgcacc ggcgggaaac gttcgctccg tgaagtcctt ggcgagcctc tcgaggtgtt
      661 tgcgatccag acccgagagc atcggcacgt gcgcaagcgc atcaaccaca ttcgtcgaca
      721 tggcgggatg cttcaccccg gctcgacgtc tgtcaatggc gagtcgcacc gtgggacgcg
      781 gaaggcgtcg attccgctgt cgaaaccagc ggaccggacg cccgaccacc tcgccgacgg
      841 tcagcgttgg taccgtctgt ccatggccga cccatccgag cccacatccc acatcgagca
      901 ctacgccaac ggccggctga agatgaccgg cttccacctc gccggcgaga tgcacggtga
      961 ctgggagtgg taccgcctcg acggcagcct gatgcggacc gggtcgttcg atcgggggcg
     1021 ccagagcggt ccgtggcgga ccttcgatcg agccggccga atggtcaagg agaccgactt
     1081 cggcgaggcc gcgaagggct gagcggccgc cccgacgatc cgcgaaatcg ccccggcagg
     1141 cgtaatccga gcgacaggtg cgcgcggcga gactctgaca tggacgcaac gacgatgtcc
     1201 ggccgggcga tccgcgccgg ttccagaacc tgctcgggag atctctccgt gaacgaaccc
     1261 aatgacgtct ggtcccctgc cggggccacg acggacgacc tcgccgcacc cgcggctccg
     1321 cttcgaaccg gtcggcgtgc caccgggcgc cagaaagcga agctcgcggg cgcgctgatc
     1381 ctcggcgggg tcgccggcgt ggcgatcctc ggaccgctct cggtcctggc agcttcgccg
     1441 tctccggccg cgtcgtcagg cgcgacaacg gggaccaccg gctccggcgg atcgaccggc
     1501 agcggaatct ccggcgcggc cggaacgaac gaccagcgcg gtcccggcgg tcccggcggc
     1561 ttcggtcgca acgaggccgt cagcgatgaa tcggtcgtcg ccaaggcgat cgggatcagc
     1621 gaagccgacc tcaagacggc cctcgccggt ggccagaccg tggccgctgt ggccaaggcc
     1681 cacagcgtcg acatccaggt cgtcatcgac gccctcgtcg ccgacggcca gagcgaactc
     1741 gatgccgccg tcaaggccgg cacgctgacc caggcccagg ccgatgccga gaaggccaac
     1801 ctgacccagc gggcgaccga ccaggccaac ggcaccttcg gcggcgggcg ccactgaccc
     1861 atcgatctcc tctcctcctc tcctcccggc cggccgcgtc atccgcggcc ggccgctcca
     1921 tgttcgagcc gtcatcgccg tcccgcgccg gatcgtcgag cgccggcggg cggagcgggg
     1981 gatgatgcgt cgcgaggagg tgccatgttc gtcccgctgt cggtgctcga gttccgcgac
     2041 cgagccgcgc tgttcttcgg cgacaaggtc ggcatcatcg acggcgagcg ccagttcacc
     2101 tatcgggcgt ttgccgagcg aacgcatcgg ctcgccaatg ccctgcgcgg gatcggcgtc
     2161 ggagccggcg accgtgtctc gttcatcacc tacaacaccc accagctcct ggaggcgtat
     2221 tacggggtcc tcgaggcagg cgcggtcctg aacccgatca acatccggtt ggcggcgcat
     2281 gagatcgcct tcatcctcga tcacgccgcc tcccgggtgg tggtcttcca tcacgatttc
     2341 ctgcccctga tcgagcagat cggcccgcgc ctcgccagcc ggcccacctt cgtcgtcctc
     2401 gagggcgacg ccggcggcct cgtcgatcac gagtacgagg cgctcctggc cggcggctcg
     2461 gccgacccgc tccatccgga gatcgacgag aacggcctgg ccgagctctt ctacacgagc
     2521 ggcacgaccg gcatgcccaa gggtgtcgcg atgacccatc gcgagctgta cctccacagc
     2581 ctcgtggccg aggtcgggct cggcttcgac caggacgatg tcgtcctcca tgtcgtgccg
     2641 ctgttccacg tcaatggctg gggaacaccg catttcgtga cgatgatcgg cggccgccat
     2701 gtcatgctcc ggcggttcga cccgggcgcc ctcatggagc tcgtcgagcg ccaccgcgtc
     2761 acccgtctgc tcgccgttcc cacgatcttc aacgcgatcc tcaaccaccg cgagcgatcc
     2821 agccgtgacc tgacgagcct ccgccaggtc atcatcggcg gcgcccccgc ctcgccggcc
     2881 ctcgtcgcat cgctggaggc ggagctcggc gtccaggcga tcgtcggcta cggcctcacc
     2941 gaaacgtcgc cgatcatcac cctcgcccag ccacggagct tcctcaccga acacgagccg
     3001 gccgaacgcc gccaggagcg gcagtccatg accggctggg ccatcccggg tgtcgctgtc
     3061 cgcgtcgtcg atggggacgg gcacgacgtc cgacccgacg gcgaacagat cggcgagatc
     3121 gtcgtccgct cgaacctcgt catggacggc tactaccgcg atcctgacgc caccgcgaag
     3181 gccatccgcg gcggttggct ccacaccggc gacatggcca cgatcgacga agccggctac
     3241 gtgctgatca aggatcggtc gaaggacatc atcatcagcg ctggcgagaa catctcctcg
     3301 gtcgagatcg aggtcgccct cggcgcgcat ccggccgtcc tcgaatgtgc cgtgatcgcg
     3361 gcgccggacg acaagcgggg cgaggttccc gtggcgttcg tcgtcctcaa gcccgaccag
     3421 actgtgtcgg ccagcgacct gcgggcgttc tgtcgcgaag ggttggccgg cttcaaggtg
     3481 ccgcgccaga tccagttccg cgacgccctg ccgaagggcg gcacgggcaa gatcctgaag
     3541 gccgagctgc gcgaaccgtt ctgggtcgga cggagctcgc gcgtcaactg agggtcacag
     3601 gttgcgaacc gacggcggct cacgcgcgag tcggctgctg agcgccgtcc ggaaggatgc
     3661 cgctcagcct cgtcggggcc tgctcgaatt cgggagcagg ttcctcgttc cgcaggccgg
     3721 gcagaccgcg gagctgcgga agaacactcc gatgctcgtg aaccgcgtcc ggcagaagcc
     3781 gcaggtcagc tcccagggcg ccattcgcgc gacccacagg aaggtcgtcc acgtgtcggt
     3841 gcggaccggg ttgatcgcgt cgatcttgag ctgggccatc ggtcagccgc tgatctggcg
     3901 cttgcggcga gcgacgaatg cggcgatggc ctccgcgacg gccgggtcga gtggcggcgg
     3961 ctcgaactcg gcgaggagct gcttccagat tcggttcgcc cgctgggtcg ccgtctgcgc
     4021 cccgtcgtcc aaccaggtct cgtagttccg ccagtcggac aggatcggcc ggtagaaggc
     4081 ggtctcgaac cgctcgagcg tgtgggccga cccgaagaag t
//
LOCUS       DATAIW010000338         6796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_21367, whole genome shotgun sequence.
ACCESSION   DATAIW010000338 DATAIW010000000
VERSION     DATAIW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21367"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VHM48_10390"
     CDS             complement(<1..552)
                     /locus_tag="VHM48_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2755863.1"
                     /translation="MAEAVRLRILAGVLVAALIGLPLVRLVAVAVGRGVPEALASLTD
                     PAVAGAIGNSVWTAAVVAILAVIGGTVVALATERGGLPGRSWLRAGILLPLLCPPFVT
                     AFGWTRAYGPRGLADQIAGIEVPGLYGPLGIVLVLAVSAMPLAWLIVAGALASRVEPD
                     VERAARAAGAGPWTAIRTVTLPLL"
     gene            complement(545..1603)
                     /locus_tag="VHM48_10395"
     CDS             complement(545..1603)
                     /locus_tag="VHM48_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2755864.1"
                     /translation="MSIASTATGRRHRSPLVLFTALALIAAACSAAVTPVPTATNSSP
                     STAPGGPSTTPIASGTITLYTSVTQNTVDAVVTGFTDAHPGTDVEVFRAPTAEVAARI
                     ATDLASGGLKADVLWLTDPLSIAAYADQGLLQTWTPPSAAAIPAAYRTDTFFGTRLLN
                     MVLVHNTSRADGPADFSDLLAEDFRGSIAIPDPGFAGSAYAALAWFDQADGYGMAFYR
                     ALKDHGAAQVKSPDDVTTGVAEGQYQVGMTLDNSARTAIKKGSPIAMLWPSGGAIAIY
                     SPIAAVDASVNRASADSFVDFSLSLPGQALIAGTGWQPVVGPGGPPVVGPQVSPDWAA
                     AFGRQKELLASYRAIFGG"
     gene            complement(1760..2614)
                     /locus_tag="VHM48_10400"
     CDS             complement(1760..2614)
                     /locus_tag="VHM48_10400"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX2755865.1"
                     /translation="MNPLADPTMVPPATPALPDPSLPPADAVEIPTNGAILFARYAYP
                     PNELGYCGPPDHKALLDYGAEGTVDPGLVELARGFAGAWPYLEFIAGATGLKSPLDRR
                     VVEAYWLGGELLDRIDMVRFGNSLLDRFRRRAGRGWGYLAEAIPEGAVPNHAFHVFGV
                     YPWVGLLKTGRASTPLEHLERCRIRWGQVVSADGDEVTVLSRPLTYDGTDIGLGERRL
                     ETARRSIDGVGFLDPFRPGDWVSLHWGWVCDRLSRRQLQLLRQSTLRQFEITNRRVVH
                     SGPRDGIG"
     gene            complement(2611..2964)
                     /locus_tag="VHM48_10405"
     CDS             complement(2611..2964)
                     /locus_tag="VHM48_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2755866.1"
                     /translation="MCLGIPGRIIERSPDHANLASVEVEGIVREINIALLEGDPQPGD
                     WVLIHLGFALETMTEEEARDAMATAAILAEGGTDDPLVPFRFADEDDTLAAPGFGRAS
                     PPTVADAAVREEARS"
     gene            complement(3080..3904)
                     /locus_tag="VHM48_10410"
     CDS             complement(3080..3904)
                     /locus_tag="VHM48_10410"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX2755867.1"
                     /translation="MGQLPPAAPGRDLLRPLRLFALFFRIGALNELQYRANLAVQLFQ
                     SAIALGTGLAVLGLVFGQTTNLGGWSQSELLAVMGVHILMGGVIRTAIQPNMERLMTD
                     IRKGTLDFVLTKPEDAQLMVSVREFRIWQGVDVLVGAIVLAVAVAQLGTGIGLGAGLV
                     FASTLLLGGVMVYCFWLLVTTAAFWVIRMDEIHELFEGLYQSGRWPVTIYPGWLRISL
                     TFLVPIAFAVTVPAEALTSRLTIETLALAAAFAVGLFLVTRWFWRFGLRNYSGASA"
     gene            complement(3904..4752)
                     /locus_tag="VHM48_10415"
     CDS             complement(3904..4752)
                     /locus_tag="VHM48_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX2755868.1"
                     /translation="MTTTAPTIGRGPLAAARGWVGLYTTQMRIAILSQIQYRVSNYFY
                     MIGMVTEPVIYLVVWSTVARQQGGAVGGFTPGEFAAYYIVWTLVRNMNIALTPWAWEE
                     RVRGGDLAVMLLHPVHPIHTDLAGLAGWKAVVILLWLPIAAFLSLIFQPTFTIEPIDV
                     AVFLVAIWGAYVLRSMFLWLLGLVTFWTTRVGPVFDIFFVAELLLSGRLVPLSLMPDW
                     AQRLADVLPFKWTFSFPIEALIGRLPPDQLLGGLAMQVLWIAISASIVAVCWRLAVRR
                     FSAVGG"
     gene            complement(4761..5780)
                     /locus_tag="VHM48_10420"
     CDS             complement(4761..5780)
                     /locus_tag="VHM48_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2755869.1"
                     /translation="MEAQPVPDPAIRVAELRKTFRVPVREAGLGAAVRSLFRREHREV
                     HAVDGVSFAIEPGEVVGFLGPNGAGKTTTLKMLSGLLYPTSGEATVLGHTPSRRQGDF
                     LRRITMVMGNRNQLQWDLPALDSFELIRAIYRLEPAAFRATRDEFIELLDLGDLVTKP
                     VRNLSLGERMKMEFVGALLHRPQVLFLDEPTLGLDVTMQKRIRSFVADYNRRFGATVL
                     LTSHYMADVQALCRRVIVIHHGRILFDGALAELSTRFDSTKTIGVTLSDGIADLSAYG
                     EVLSTDDGRTVLRVSRADAPDVTTRLLRDLPVADLTIEDPPIEDVIERVFADARDERA
                     TAVPG"
     gene            6139..6606
                     /locus_tag="VHM48_10425"
     CDS             6139..6606
                     /locus_tag="VHM48_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2755870.1"
                     /translation="MDQTERTALIERYRTGTADVESALVGITDAELDRRPGEPDAWTA
                     REIAHHLADSEAMAYIRLRRLLAEDAPLIAGYDEPEWARRLHYDRPIASSLAVLAAVR
                     ASSLELLERLAAADWARAGTHSDSGSYGVEDWLRIYADHAHHHADQIRAARGG"
BASE COUNT         1084 a         2345 c         2491 g          876 t
ORIGIN      
        1 gagcagcggc agggtcacgg tccggatcgc cgtccacggc cccgcgccgg ctgcccgcgc
       61 cgcccgctcc acgtccggct cgacccggct ggcgagggca cccgccacga tcagccaggc
      121 cagaggcatg gccgacaccg ccagcaccag gacgatcccg agcggaccgt acagcccggg
      181 cacctcgatg cccgcgatct ggtcggcgag tccgcgtggc ccgtaggcgc gggtccagcc
      241 gaacgccgtc acgaatggcg ggcagagcaa cggcaggagg atccccgccc ggagccagct
      301 gcggcccggg agcccgccgc gctcggtcgc gagggcgacg accgttccgc cgatgacggc
      361 gaggatggcc acgaccgccg cggtccagac gctgttcccg atcgcgcccg cgacggccgg
      421 atcggtgagg ctggcgaggg cctccgggac gcctcgcccg acggcgacgg cgacaagccg
      481 gacgagcggc agcccgatca gtgcggcgac gaggaccccg gcgaggatcc tcaggcgtac
      541 ggcttcagcc accgaagatg gcgcggtacg aagccagcag ctccttctgc cggccgaacg
      601 ccgccgccca gtccgggctc acctgcggcc cgacgaccgg cgggccgccc ggcccgacga
      661 cgggctgcca gccggtcccg gcgatcaggg cctggccggg cagtgacagg ctgaagtcga
      721 cgaacgagtc ggcgctcgcc cggttgaccg acgcgtcgac cgcggcgatc gggctgtaga
      781 tggcgatcgc gccgccggac ggccagagca tcgcgatcgg cgagcccttc ttgatcgccg
      841 tccgggccga gttgtcgagc gtcatcccga cctggtactg gccctcggcg acgcccgtcg
      901 tgacgtcgtc cggcgacttg acctgggcgg cgccgtggtc cttcagggcc cggtagaagg
      961 ccatcccata gccgtcggcc tggtcgaacc aggcgagggc ggcgtaggcc gagccggcga
     1021 agcctgggtc cgggatcgcg atcgaacccc ggaagtcctc ggcgagcagg tcgctgaagt
     1081 cggccgggcc gtcggcacgg ctcgtgttgt ggaccaggac catgttcagc agccgcgtcc
     1141 cgaagaacgt gtccgtccgg tacgcggcag ggatcgccgc ggcgctcggc ggggtccagg
     1201 tctggagcag gccctgatcg gcgtaggcgg caatcgaaag cgggtcggtc agccacagga
     1261 cgtccgcctt gagcccgccc gacgcgaggt cggtcgcgat ccgtgccgcc acctcggccg
     1321 tcggcgcccg gaagacctcg acgtcagttc ccgggtgggc gtcggtgaag cccgtgacca
     1381 cggcgtcgac cgtgttctgg gtgaccgagg tgtagagggt gatcgtgccg ctcgcgatcg
     1441 gcgtggtcga cgggccgccg ggcgccgtcg atggcgagct gttggtcgcg gtcggcaccg
     1501 gggtgacggc cgcggaacaa gccgcggcga tcagcgccag agcggtgaac aggacgaggg
     1561 gggaacgatg ccggcgcccg gtcgccgtcg atgcgatgga catgaaggag ctcctcgtgg
     1621 cgaagcggac gtcatcgggg acggctgaat gccgagaacc cggcttccac gcttgcccgc
     1681 gctggggcgt catcgatgct gctggtccac cacctccgct gtcagcgtcc aaccggcccc
     1741 ctcgttcggg accaagggct cagccgatcc cgtcgcgcgg cccgctgtgg acgacccgcc
     1801 ggttggtgat ctcgaactgg cgcagcgtcg attggcggag cagctggagc tgacgccggc
     1861 tcaaccggtc acagacccag ccccagtgga gcgacaccca gtcgcccggt cggaacggat
     1921 cgaggaagcc gactccgtcg atcgaccgcc gcgccgtctc cagccgccgc tcgccgagcc
     1981 cgatgtcggt cccgtcgtag gtcagcgggc gggacaggac cgtcacctcg tcgccgtcgg
     2041 ccgaaacgac ctggccccag cggatccggc acctctcgag gtgctcgagc ggggtggacg
     2101 cgcggcccgt cttcagcagg ccgacccacg ggtagacgcc gaagacgtgg aacgcatggt
     2161 tcgggaccgc gccctcgggg atcgcctcgg cgagatagcc ccagccgcga cccgcccgcc
     2221 gccggaaccg atcgaggagc gagttgccga agcggaccat gtcgatccga tccagcagct
     2281 caccgccgag ccagtaggcc tcgacgacgc gccggtcgag cgggctcttc agcccggtcg
     2341 ccccggcgat gaactcgagg tacggccagg cgccggcgaa gccgcgggcg agctcgacga
     2401 ggccggggtc gaccgtcccc tcggccccgt aatcgaggag cgccttgtgg tcgggcggac
     2461 cgcagtaccc gagctcgttg ggtggatagg cgtagcgggc gaagaggatc gccccgttcg
     2521 tcgggatctc gaccgcgtcg gccggcggca ggctgggatc gggcagggcc ggcgtcgccg
     2581 gtgggaccat cgtcgggtcg gccagcgggt tcacgagcga gcttcctccc gaacggccgc
     2641 gtctgcgacg gtcgggggag atgcccggcc gaacccgggc gccgcgagcg tgtcgtcctc
     2701 gtcggcgaag cggaagggca cgagcggatc atccgtcccg ccctcggcca ggatcgccgc
     2761 tgtcgccatg gcgtcgcgcg cctcctcctc cgtcatcgtc tcgagggcga acccgagatg
     2821 gatcaggacc cagtcgccgg gttgcggatc gccttcgagg agggcgatgt tgatctcccg
     2881 gacgatgccc tcgacctcga cgctggccag gttggcatga tcaggcgatc gctcgatgat
     2941 tcggcccggg atcccgaggc acatcaggct gcgctccttc cggaacgacc ctgccacatc
     3001 tcgcgaccac gcaggcaccg ccggttgtca cattccatca tgaccggtcc cgggtccgag
     3061 gggacgaacc ggcccccggt caggccgacg cgcccgagta gttgcgcagc ccgaagcgcc
     3121 agaaccagcg ggtcacgagg aagaggccga cggcgaaggc cgccgcgagg gcgagcgtct
     3181 cgatcgtcag gcgcgacgtc agggcctcgg ccgggacggt gaccgcgaac gcgatcggga
     3241 cgaggaacgt caggctgatc cggagccagc ccggatagat cgtgacgggc cagcggccgg
     3301 actggtacag accctcgaac agctcgtgga tctcgtccat ccggatcacc cagaacgccg
     3361 cggtcgtcac gagcagccag aagcagtaga ccatcacgcc gccgaggagc agcgtgctcg
     3421 cgaagaccag accggcaccg agcccgatac ccgtcccgag ctgggccacc gccacggcca
     3481 ggacgatggc gccgacgagg acgtcgacgc cctgccagat ccggaactcg cggacgctga
     3541 ccatcagctg ggcatcctcc ggcttggtca ggacgaagtc cagggttccc ttccggatgt
     3601 cggtcatcag gcgctccatg ttcggctgga tggccgtccg gatgactccg cccatgagga
     3661 tgtggacccc catcaccgcg agcagttcgg actggctcca gccaccgagg ttcgtcgtct
     3721 ggccgaagac gagccccagg acggcgaggc cggtgccgag cgcgatggcc gactggaaca
     3781 gctggaccgc gaggttcgcc cggtactgga gctcgttcag cgcgccgatc cggaagaaca
     3841 gcgcgaacag gcggaggggg cgcagcaggt cgcgtcccgg tgcggcgggc gggagctggc
     3901 ccatcagccg cccaccgccg agaagcgccg gaccgcgagc cgccagcaga ccgcgacgat
     3961 cgacgcgctg atcgcgatcc acaggacctg catcgcgagg ccgccgagga gctggtccgg
     4021 aggcagccgc ccgatcagcg cctcgatcgg gaagctgaag gtccatttga acggcaggac
     4081 gtcggccagg cgttgggccc agtcgggcat cagcgacagc ggcaccaggc ggccggacag
     4141 caggagctcg gcaacgaaga agatgtcgaa gaccggcccg acccgggtcg tccagaacgt
     4201 cacgaggccg agcagccaga ggaacatcga gcgcaggacg taggcgcccc agatcgcgac
     4261 caggaacacg gcgacatcga tcggctcgat cgtgaacgtc ggctggaaga tcagcgacag
     4321 gaacgcggcg atcggcagcc agagcaggat gacgaccgcc ttccaaccgg cgagcccggc
     4381 caggtcggtg tgaatgggat ggaccgggtg aaggagcatc acggcgaggt ccccgccgcg
     4441 gacgcgctcc tcccaggccc atggcgtgag cgcgatgttc atgttccgga ccagggtcca
     4501 gacgatgtag tacgcggcga actcgccggg cgtgaagccg ccgaccgctc cgccctgctg
     4561 ccgagcaacg gtcgaccaga cgacgaggta gatgaccggc tcggtgacca tcccgatcat
     4621 gtagaaatag ttcgagacgc ggtactggat ctggctcagg atcgcgattc gcatctgggt
     4681 cgtgtacagg ccgacccagc cgcgcgcagc agccagcggc ccccgcccga tcgtcggcgc
     4741 cgtcgtcgtc atcgtcgggg tcagccggga accgcggtcg cccgttcgtc ccgggcgtcg
     4801 gcaaagaccc gctcgatgac gtcctcgatc ggcggatcct cgatcgtcag gtcggcgacc
     4861 ggcaggtcgc gcaggaggcg cgtcgtgacg tccggggcat cggcgcgcga gacgcgcagg
     4921 accgtccggc cgtcgtcggt cgagaggacc tcgccgtagg ccgagaggtc ggcgatgccg
     4981 tcgctcaggg tgaccccgat cgtcttcgtc gagtcgaacc gggtcgagag ctcggccagg
     5041 gcgccgtcga acaggatccg gccgtggtgg atgacgatca cccgtcggca gagggcctgg
     5101 acgtcggcca tgtagtggct cgtgaggagg acggtcgcgc cgaaccgccg gttgtagtcg
     5161 gcgacgaacg agcggatccg cttctgcatc gtgacgtcga ggccgagcgt cggctcgtcg
     5221 aggaacagga cctgcggccg gtggaggagc gccccgacga actccatctt catccgctcg
     5281 ccgagcgaca ggttgcggac cggcttggtg acgaggtcgc cgaggtcgag gagctcgatg
     5341 aactcgtcgc gggtcgcccg gaaggccgcc ggctcgaggc ggtagatcgc ccggatcagc
     5401 tcgaacgaat cgagggccgg gaggtcccac tggagctgat tgcggttgcc catgaccatc
     5461 gtgatccggc gcaggaagtc gccctggcgg cgagaaggcg tgtggccgag gaccgtggcc
     5521 tcaccgctcg tcgggtagag gagaccgctg agcatcttga gggtcgtcgt cttgccggcg
     5581 ccattcgggc cgaggaagcc gacgacctca ccgggctcga tcgcgaagga cacgccgtcg
     5641 acggcgtgga cctcccgatg ctcgcgtcgg aacaggctcc gcacggcagc tccgagaccc
     5701 gcctcgcgga cggggacgcg gaaggtcttg cggagctcgg cgacgcggat cgcgggatcg
     5761 ggcacgggct gggcctccat gggcacgggg agggttccgt agggtaccgc gtcactgggt
     5821 gagcgccacc cccggcgcgg tcgccggcgc cggtcgtggt ccgacctggc caaacggtcg
     5881 cccgggggct cttgagccag gtttccgcgt gcaactggcg cgatcgggag ctcgagtttg
     5941 ccctcgccgg ctaacggagc tcgtcgaatc gcgggcagga gccacgggag ctgacacaag
     6001 agtccaccag tgactgtttg gccagattcc gtagatcgag tttgctcgtc accgggcgcc
     6061 ggcgccccgt cgccggcgcc cgacgctcga cggctggtgc ccggccggtt cggcgatact
     6121 cgctcctgag gaggacccgt ggaccagacc gaacgaaccg cactcatcga gcgctaccgg
     6181 accgggaccg ccgacgtcga gtcggccctc gtcggcatta ccgacgcgga gctggaccgc
     6241 cggcccggcg agcccgacgc gtggacggcg cgcgagatcg cccatcatct cgccgacagc
     6301 gaggcgatgg cctacatccg gctccgccgc ctcctcgccg aggacgcgcc gctgatcgcc
     6361 ggctacgacg agcccgagtg ggcgcggcgg ctgcactacg accggccgat cgcgtcgtct
     6421 ctggcggtgc tggcggccgt ccgggcatcc agcctggagc tcctcgagcg ccttgccgcg
     6481 gccgattggg cgcgggcggg cacgcacagc gactccggct cgtacggggt cgaagactgg
     6541 ctccggatct acgccgacca cgcccaccat cacgccgacc agatccgggc ggcccgaggc
     6601 ggctgaaagc gtggtcgtcc ggcgccggcg agccgtcgcg cgtcagtccg ggacggccgc
     6661 ggctgcgaac gcggccatcg acgggtaggt cgcatccggg cgggcatcgg ccgggggtgt
     6721 cgcgccgcca cccgggcggt cgtggcggcg atcgatccag accgagcgga agccgagccg
     6781 ctgggccggc gcgtga
//
LOCUS       DATAIW010000339         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_217972, whole genome shotgun sequence.
ACCESSION   DATAIW010000339 DATAIW010000000
VERSION     DATAIW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217972"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHM48_10430"
     CDS             complement(<1..431)
                     /locus_tag="VHM48_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755871.1"
                     /translation="MRALQTAGPSRRWLLAAALGSMLAMVCVAAFLPAIAFGGVWSSI
                     AVIPIGAFIVVGLADRDAIVARRAIIEYAGVQAGRRPQGYPTDREAADAWLADPDHAG
                     ENPVRRAYILVLAERADDARAELDRVDESDLSDVASAERLRA"
     gene            complement(470..1276)
                     /locus_tag="VHM48_10435"
     CDS             complement(470..1276)
                     /locus_tag="VHM48_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2755872.1"
                     /translation="MTDLIRLERSGPNDAVARVTLSRPDVHNAFNASLIAELRRTFTM
                     LAREGPTDLRVVVLAGDGPSFCAGADIDWMRAAMQLDVEGNEQDAMAFADTLEAIDIC
                     PVPVIARVHGPALGGGSGLCAVSDLVIAEAGAKFGFTETRLGILPAVISPFVIAKIGE
                     TNARALFPGGRRFDAIRAQRIGLVHEVVEGVEALDAAVDQAVADILASGPTAVRAAKA
                     IVREVRGLGHGSTKWHTARVIARQRVSAEAQEGFSAFTEKRRPAWAPDPE"
     gene            complement(1286..2605)
                     /locus_tag="VHM48_10440"
     CDS             complement(1286..2605)
                     /locus_tag="VHM48_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2755873.1"
                     /translation="MTSGPPTGGVVQVNVSPGGVPKLPVAEARVGRNGLDGDAHDHDA
                     VHGGPHRAVCLFASEAMERVRADGHPGVGPGSVGENLTTAGIELSLLDVGTRLAIGGE
                     VVLEISGPANPCDVIKGAFSGGKSGRISILLHPSDSRMYARVEREGIVRPGDSIGVLP
                     ARPETAAAVHRELDLLDSVERDAWTAMWRAAAAAGIDVRILERGDFAAAASPDLPGSV
                     FNRAFGMRQVPIHRPTMEAHFRAAGTPGWLVVAADDQAFAGRELEWPVGVHVAPIEEV
                     LGRIEGGAAPAADGLEIRRVDPDDVDDVARWADLFIAGFGIEGPLADAWRQFNPFLVR
                     SRGYHQFIGSLDGRDIAASALFNRRRVAWLGAGTVLPAARGRGIQRALIIDRVQHALE
                     AGSTRVMATAEIDTVSAANLERLGIHRIWTRGHARVDPVATHESPGR"
     gene            complement(2602..>3235)
                     /locus_tag="VHM48_10445"
     CDS             complement(2602..>3235)
                     /locus_tag="VHM48_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755874.1"
                     /translation="EEATMITFWSETPGRRRREIAADVATWAWVATWSVIAFRIHAAI
                     AGFAEAGHALERGGAAIRDAGTQVGSSLEGLPLVGQGAGEVARRAFGGASEPFVFVGG
                     EFVDLITLIARVLAILVLAVAIVPWLSRYLPWRAARLAELRSATAAIRGRPRGVTREQ
                     IDRLLASRALHRLSWAELLDHSRDPLGEFAAGRYKALARAELESVGLLPR"
BASE COUNT          491 a         1192 c         1146 g          406 t
ORIGIN      
        1 gcccgaagtc gctcggccga ggcaacgtcg gacaggtcgg actcgtcgac ccgatcgagc
       61 tcggcccgag catcgtcggc ccgctcagcc aggacgagga tgtaggcgcg cctgacagga
      121 ttctccccgg cgtgatccgg atcggcgagc caggcatcgg cggcctcgcg gtccgtcggg
      181 tatccctggg gccggcgtcc cgcctggacc ccggcgtact cgatgatcgc ccggcgagca
      241 acgatggcgt cccgatcggc gaggccgacg acaatgaacg ccccgatcgg gatgactgcg
      301 atcgaactcc agacaccgcc aaatgcgatc gcgggcagga aggccgcaac acagaccatc
      361 gcgagcatgc tcccgagggc ggccgcgagc agccaccggc ggctgggtcc ggccgtctgg
      421 agggcccgca ccccgggatt cggagctcga cgccgacgca ggatggcggt cactcgggat
      481 cgggcgccca cgcgggccgg cgcttctcgg tgaaggcgct gaacccttcc tgggcctcgg
      541 ccgacacgcg ctggcgggcg atgacccggg cggtgtgcca tttcgtcgag ccatggccga
      601 gcccgcggac ctcgcggacg atcgccttgg ccgcgcgcac cgccgtcggg ccggaggcga
      661 ggatgtcggc gaccgcctgg tcgacggccg cgtccagcgc ctcgacgcct tcgacgacct
      721 cgtggacgag cccgatccgc tgcgcccgga tcgcgtcgaa gcggcggccg ccggggaaca
      781 atgcccgggc gttggtctcg ccgatcttcg cgatcacgaa cggcgagatc acggccggca
      841 ggatcccgag tcgcgtctcc gtgaatccga acttcgcgcc ggcctcggcg atgacaaggt
      901 cgctgaccgc gcagagcccg gagccgccac cgagcgcggg cccgtgcacc cgggcgatca
      961 ccggcacggg gcagatgtcg atcgcctcga gggtgtcggc gaatgccatc gcgtcctgct
     1021 cgttgccctc gacgtcgagc tgcatcgcgg cccgcatcca gtcgatgtcg gcgccggcgc
     1081 agaacgacgg accgtcgccg gcgaggacga cgacgcggag atcggtcggg ccttcgcggg
     1141 ccagcatcgt gaaggtccgc cgcagctccg cgatcaggct cgcgttgaag gcgttgtgga
     1201 catcggggcg ggagagcgtc actcgggcca ccgcgtcatt cgggccgctg cgctcgaggc
     1261 gaatgagatc agtcaccggc gcatcctacc ggccgggcga ctcgtgagtg gccaccgggt
     1321 cgacccgggc gtggccgcgc gtccagatcc ggtggatccc cagccgttcg aggttggcgg
     1381 ccgacacggt gtcgatctcg gcggtcgcca tcacccgggt cgacccggcc tcgagagcat
     1441 gctgaacccg atcgatgatc agggcccgct ggatcccgcg gccgcgcgcc gcgggcagga
     1501 cggtcccggc gccgagccag gcgacccgcc gtcggttgaa cagtgccgag gccgcgatgt
     1561 cgcgcccatc gagcgagccg atgaactggt ggtagccgcg tgaccggacg aggaacgggt
     1621 tgaactgccg ccaggcatcg gcaagcggcc cctcgatccc gaaaccggcg atgaacaggt
     1681 cggcccagcg cgccacatcg tcgacgtcgt ccgggtcgac ccgccggatc tccaggccgt
     1741 cggcggccgg cgcggcgccg ccttcgatcc gcccgaggac ctcctcgatc ggtgccacat
     1801 ggaccccgac cggccactcg agctcccggc ccgcgaacgc ctggtcgtcg gccgcgacaa
     1861 cgagccagcc gggcgtcccg gccgctcgga agtgcgcctc catcgtcggc cggtggatgg
     1921 gcacctggcg catcccgaag gctcggttga agacagagcc gggcaggtcg ggcgaggcgg
     1981 ccgccgcgaa atcgccgcgc tcgaggatcc ggacatcgat cccggcagcg gccgccgccc
     2041 gccacatcgc ggtccacgca tcacgctcga cgctgtcgag aaggtcgagc tcgcgatgga
     2101 cggccgcggc cgtctcgggt cgcgccggca agacgccgat cgaatcaccg ggccggacga
     2161 tgccttcgcg ctcgacacgc gcgtacatcc gcgagtcgga cgggtggagc aggatcgaga
     2221 tccgacccga cttgccgccg ctgaacgcac ccttgatcac gtcgcacggg ttggccggcc
     2281 cggagatctc gagaacgact tcgccgccga tcgccagccg cgtcccgaca tcgagcaggg
     2341 agagctcgat accggccgtc gtcaagttct cgccgaccga cccggggccg acccccggat
     2401 ggccgtcggc ccggacccgc tccatcgcct cgctcgcgaa caggcagacc gcccgatgcg
     2461 gcccgccgtg gacggcgtcg tggtcatggg cgtcgccgtc gagcccgttg cgtccgaccc
     2521 gcgcctcggc caccggcagc ttgggcaccc cgcccggcga gacgttgacc tggacgacgc
     2581 cgcctgtcgg cggtcccgac gtcatcgcgg cagcagtccg acagactcga gctcggcccg
     2641 ggccagggcc ttgtaccggc cggccgcgaa ttcgccaaga gggtcccggc tatggtcgag
     2701 cagctccgcc caggacaggc ggtggagtgc ccggctggcg aggaggcgat cgatctgctc
     2761 gcgggtcaca ccgcgcgggc gtccgcgaat ggcggccgtg gccgaccgga gctcggcgag
     2821 gcgcgccgct cgccacggca gatagcggct cagccacgga acgatcgcaa ccgccaggac
     2881 gaggatcgcg aggactctcg cgatcagcgt gatcaggtcg acgaactcgc cgccgacgaa
     2941 cacgaacggc tcactggcgc caccgaaggc ccgtcgggcg acctcgccgg cgccctggcc
     3001 gaccagcggc aggccctcga gggaggaccc gacctgggtt cccgcatcgc ggatcgccgc
     3061 cccgccacgc tccagggcat ggcctgcctc ggcaaagccc gcgatcgcgg catggatccg
     3121 gaacgcgatg acggaccagg tcgcgaccca cgcccaggtc gcaacgtccg ccgcgatctc
     3181 ccgtcgtcgc cggccgggcg tctcactcca gaatgtgatc atcgtcgcct cctcg
//
LOCUS       DATAIW010000340         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_21424, whole genome shotgun sequence.
ACCESSION   DATAIW010000340 DATAIW010000000
VERSION     DATAIW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21424"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHM48_10450"
     CDS             complement(<1..278)
                     /locus_tag="VHM48_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755875.1"
                     /translation="MAQLQDIWKKFNPRERLSAIGAGLIILAWIVGIFSYGLGSSTIA
                     LLGAIAVLAILYIKYAPTMNVTWPAPVSLLILGISGVVALLALLDLLT"
     gene            397..1149
                     /locus_tag="VHM48_10455"
     CDS             397..1149
                     /locus_tag="VHM48_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2755876.1"
                     /translation="MTTPRDWDAATYDRVADPQTRWGLAVLDRLPLHGDETVLDAGCG
                     SGRVTELLAERLPRGRVIALDASPSMVEEARRRLVGFGARIAYLVADLGRPFALPGSE
                     PVDAILSTATFHWVADHDALFRNLAAVVRPGGRLVAQCGGAGNVASIMAILATIGDGW
                     LGGVHFATPEATTARLAAAGFVDIEAWLQPEAAAFDPGEPFETFLRTVVLGNHLARLP
                     EEEHDAFVRAVATRLPEPVIDYVRLNITATRG"
     gene            complement(1373..1927)
                     /locus_tag="VHM48_10460"
     CDS             complement(1373..1927)
                     /locus_tag="VHM48_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2755877.1"
                     /translation="MTTVELIHAGYIRDDSRVGSSISLIRDGDAIIVADPGLVASHAR
                     ILDPLRERGIAPGDVTHVTFSHHHPDHTMNAALFPNAEVIDFWARYRNDLWLDHPGDG
                     WDITDHVQLWLTPGHTEEDLSLIVHADDAVYALTHLWWRQDRTPEIDPYAPDQAILET
                     HRARVLAVADIVIPGHGEAFRVDR"
     gene            2026..3678
                     /locus_tag="VHM48_10465"
     CDS             2026..3678
                     /locus_tag="VHM48_10465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2755878.1"
                     /translation="MTQTGAPADLVLTGGHAHTVDLLRPRAEAVAVRGERIVAVGSAA
                     DVADLVGPRTRVVNLAGRLLLPGFQDAHIHPIFGGVDRLQCDLRVAHGRDGVLAAIRA
                     YVATNPDDQWVVGSGWSMADFENGTPRREDLDAIIPDRPAFFPNRDGHSTWVNSRALT
                     LARIDRDTADPADGRIERDPDGTPTGTLHEGAVRAVERLMPEATADLMAHGLRIAQDY
                     LHSLGITAWQDAIVRPEDQAVYRAAAEAGWLTARVEGAMWWDRERGDEQVDELIERSR
                     TGSFGRLRVNSVKLMQDGVLETFTGAMIDPYLDGQGGTTTRRGISFIDPERLPGWVTR
                     LDAAGLQPHFHAIGDRAVRECLDAVEAARSANGRTDTRPHIAHIQVIHPDDIARFATL
                     DVVANAQPLWACHEGQMDVLTIPFLGPERTTWQYPFRSLLSAGSRLAMGSDWSVSTPN
                     PLEEMEVAVTRVYPESRGSTAPFLPRQALTLDQAIRAFTAGSAYVNHLDAETGTIEVG
                     KLADLSVIDRDLFAPDAGPIGDGRVLGTFVGGVAVFEDPTLG"
     gene            3842..4027
                     /locus_tag="VHM48_10470"
     CDS             3842..4027
                     /locus_tag="VHM48_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755879.1"
                     /translation="MNSLSHLYVAAHQNEMLNLAANERLARTQPATERPIHLGAALKS
                     VWSLLSGPAERPMTSRP"
     gene            complement(4142..4753)
                     /locus_tag="VHM48_10475"
     CDS             complement(4142..4753)
                     /locus_tag="VHM48_10475"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX2755880.1"
                     /translation="MTAPLDVAASGIVLAGGRSSRYGSDKLAARVEGATLLDRSVAAL
                     AAVAVEIVVVVAPGDDRVPAPIDRPLRLVADPELHGGPLVGLLAGLEAVDQPIALVVG
                     GDMPSLRADVLRLMLRTLVTADEAFGAVILERRGRGEPLPAVVRTGSGTDVARRLVAD
                     GERSLRSLLERLPTRIIDEAAWRPLDPDAATLHDVDRPSDLES"
     gene            complement(4750..>6541)
                     /locus_tag="VHM48_10480"
     CDS             complement(4750..>6541)
                     /locus_tag="VHM48_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX2755881.1"
                     /translation="AHLTLPDGTELDFVDVPGHDRLVGNMLVGAGEIDAAMLVIAADD
                     GPRAQTREHLELLDALGIDDGLIVVTKSDVAGPERVAEVVAAAGALVASTTLGGVPVL
                     AVSATTGEGLAALVTALVDLRDRIAIRSAGDIPDAGSRLAIDRAFSIRGRGTVVTGTL
                     RGTPIERGTLLRVVPGPADRSIRVREVQIHGRAVDRAGPGRVALNVAGDGVDGLGRGA
                     VLTTDPRVVASDRWLVALRRATAFGAGTASAAGRGPGLPADRTRIAVHLGTARVAGVV
                     GRSGRDGVDLDGGEGSAILRLDRPIAVAPGDRFVVRRPSPVETLAGGRVLDPQPARGI
                     ARRRTTVERLRALATAGAGSDAWRSARAELHGVVLDSSPGIAPDVAAAIDDVLLAAVE
                     GRPGIRISELVRAGEVELRRRVGTIGPPGAAAGDAAHRIVAGRLDDLIGADRLSRDAD
                     RVRPAGVAAVGPSPALATAMDRLVAALSTVSPPPLTSAARAAGCPPEGIRLLEQANRI
                     VRLDDDLAWAFPAWRDLAARAIELARGGPLTPAAYRDATGTSRKYVMALLEDLDRRAI
                     LRRTPAGHVPGPRAPQLAAADAASPAGAPR"
BASE COUNT          909 a         2383 c         2362 g          887 t
ORIGIN      
        1 cacgtcagca ggtcgagcag cgccagcagc gccacgacgc cgctgatccc gaggatcaac
       61 agggagacgg gcgccggcca ggtgacgttc atcgtcggcg cgtacttgat gtacaggatg
      121 gccagaacgg cgatcgctcc gagcaacgcg atcgtgctcg acccgaggcc atagctgaag
      181 atgccgacga tccaggcgag gatgatcagc cccgcgccga tcgccgagag tcgctctcgc
      241 ggattgaact tcttccagat gtcctggagt tgcgccattg atcaccctcc gttgtgttcc
      301 gtcgcggatc ggcggactgc gtccccgtct cttagggtag cgacgcggag ccgcggaatc
      361 cagacctgac gatcgttccc cctccatact tccccggtga ccacgccgcg cgactgggat
      421 gccgcgacct acgaccgggt cgccgatccg cagacccgct ggggactcgc ggtcctggac
      481 cggttgccgc tccatggcga cgagacggtc ctggatgccg gctgcggctc cgggcgggtc
      541 accgagctcc tggccgagcg tctgccgcgc ggtcgcgtga tcgcgctcga cgcctcgccg
      601 tcgatggtcg aggaggcccg tcgacggctg gtcggtttcg gtgctcggat tgcctacctg
      661 gtcgccgacc tcggacggcc attcgcgctg cccggctccg agcccgtcga cgcgatcctt
      721 tcgaccgcga ccttccactg ggtcgccgat cacgacgcac tcttccggaa cctcgccgcg
      781 gtggtccgtc ccgggggccg gctcgttgcc cagtgtggcg gcgccggcaa tgtcgcctcg
      841 atcatggcca tcctcgccac gatcggcgat gggtggctcg gcggggtcca cttcgcgaca
      901 cccgaagcga caacggctcg gctcgcggct gccggcttcg tcgacatcga ggcctggctc
      961 cagccggagg cggccgcgtt cgacccgggt gagccgttcg agacgttcct ccggacggtc
     1021 gtcctcggca accacctcgc gcgcctgccg gaggaggagc acgacgcatt cgtccgcgcg
     1081 gtggcgacgc gcctgcccga gccggtgatc gactacgtcc gcctgaacat cacggcgacg
     1141 cgcggctgag cgcagtcccc gccagggggc cccggactcg tccgattcgg ccgttggccg
     1201 cagtcatcct cgctgccggg cgccgtgcgg ccaggctccc cggaagacgt cctcccatca
     1261 ggcacatccc atccgtaccg ggcactcagg aggggaattg catcaggtac ggcccggccg
     1321 ttcgggcagg gccgccggaa tgtgctcggg tcgccggcgg actggtcggc tctcagcggt
     1381 cgacgcggaa tgcctcgccg tggcccggga tgacgatgtc ggcgaccgcc aggacccgcg
     1441 cccgatgcgt ctcgaggatc gcctggtccg gcgcgtacgg atcgatctcg ggtgtccgat
     1501 cctggcgcca ccagagatgg gtcagggcat agaccgcatc gtccgcgtgg acgatcagcg
     1561 acaggtcctc ctcggtgtgg ccgggggtca gccagagctg gacgtggtcg gtgatgtccc
     1621 agccgtcacc ggggtgatcg agccagaggt cattgcggta gcgggcccag aagtcgatga
     1681 cctcggcgtt cgggaacagg gccgcgttca tcgtgtggtc ggggtggtgg tggctgaacg
     1741 tcacatgcgt gacgtcgccc ggcgcgatcc cgcgctcgcg gagcggatcg aggatccggg
     1801 cgtgcgaggc gacgaggccc gggtccgcga cgatgatcgc gtcgccgtcg cggatcaggc
     1861 tgatgctgct gccgacccgg ctgtcgtcgc ggatgtaccc ggcatggatg agttcgacgg
     1921 tcgtcatcgg cgcagcatag gcgctcccac gacccgccga cctcgtgccg tctcgcggac
     1981 cgggccgccc ggtcgatcga tggatcgggg tgtcatcctc gcgccatgac gcagaccggg
     2041 gctcccgccg acctcgtcct gaccggcggc catgcccaca ccgtcgatct cctgcggccg
     2101 cgggccgagg ccgtcgccgt gcgtggcgag cggatcgtcg ccgtcgggtc ggcggcggac
     2161 gtcgcggacc tcgtcgggcc ccggacccgg gtcgtgaacc ttgccgggcg gctcctgctc
     2221 ccggggttcc aggacgccca catccacccg atcttcggcg gcgtcgaccg gctccaatgc
     2281 gatctccgcg tggcgcacgg tcgcgacggc gtgctggcgg cgattcgtgc ctacgtggcc
     2341 acgaaccccg acgaccagtg ggtcgtcggc agcggctggt ccatggccga cttcgagaac
     2401 ggcacgcccc gtcgcgagga tctcgacgcg atcatcccgg atcgacccgc gttcttcccg
     2461 aatcgcgacg ggcactcgac gtgggtcaac agccgggcgc tcaccctcgc ccggatcgat
     2521 cgggacacgg cggaccccgc cgacggacgg atcgagcgcg atccggacgg gacaccgacg
     2581 gggacgctcc acgagggcgc cgtgcgcgcg gtcgagcgcc tgatgcccga ggccacggcc
     2641 gacctgatgg cccacggcct gcggatcgcc caggactatc tccattcgct cgggatcacc
     2701 gcctggcagg atgcgatcgt ccggcccgag gaccaggctg tctatcgcgc cgcggccgag
     2761 gccggctggc tgactgcccg ggtcgaagga gcgatgtggt gggatcgcga gcgcggcgac
     2821 gagcaggtcg acgagctgat cgagcgatcc cggaccggct cgttcgggcg gctccgggtg
     2881 aacagcgtga agctgatgca ggacggggtc ctcgagacgt tcaccggcgc gatgatcgat
     2941 ccgtacctcg atgggcaggg cgggacgacg accaggcgcg ggatcagctt catcgatccc
     3001 gagcgcctgc cgggctgggt gacccggctc gacgcggccg ggctccagcc gcatttccac
     3061 gcgatcggcg accgggccgt ccgcgagtgc ctcgacgccg tcgaagccgc ccggtcggcc
     3121 aacggccgga ccgacacccg cccgcatatc gcccatatcc aggtcatcca ccccgacgac
     3181 atcgcccggt ttgcgaccct cgacgtcgtg gccaacgccc agccgctgtg ggcgtgccac
     3241 gaaggccaga tggacgtcct cacgattccc ttcctggggc ccgaacggac gacctggcag
     3301 tacccgttcc gctcgctcct gtcggccggg tcccgactcg cgatgggctc ggactggagc
     3361 gtgtcgacgc cgaatccgct cgaggagatg gaggtcgcgg tcacccgggt ctacccggag
     3421 agccgcgggt cgaccgcgcc gttcctgccc cggcaggcgc tgacgctcga ccaggcgatc
     3481 cgggcgttca cggccggatc ggcctacgtg aaccacctcg acgccgagac gggcacgatc
     3541 gaggtcggca agctcgccga tctgtcggtc atcgaccgcg acctgttcgc gcccgacgcg
     3601 ggtccgatcg gcgacggccg agtgctcgga accttcgtcg gcggcgtggc ggtcttcgag
     3661 gatcccacgc tcggttgacg cggcctgcgc cggccccgga tcggtccgtc agcgcggcgt
     3721 acgcgccatg ggccggatga cgtaggcgaa ttgtggtcgc gcgacggtgg gtgcggaacc
     3781 cgccacgtct gatgatctcg tcgtgatccc gagtgacggg atcggaaagg agagatcaga
     3841 cgtgaacagc ctcagccacc tctacgtcgc ggctcaccag aacgagatgc tcaacctcgc
     3901 cgcgaacgag cgactcgccc ggacgcagcc cgccaccgag cgcccgatcc acctcggcgc
     3961 cgcgctcaag agcgtctggt cgctcctgag cggcccggcc gagcggccga tgacgtcccg
     4021 accctgacca gcgacccata ccggggctga cctccacagc ctcctccgct ccctcctcca
     4081 cgcgaacgcg tccgtcctcc ggcgggcgcg ttcggcgttt ccggagatgt cgtcggggcc
     4141 atcacgattc caggtcggac ggacggtcga cgtcgtggag cgtcgctgca tcggggtcga
     4201 gcgggcgcca ggcggcctca tcgatgatcc gggtcggcag gcgctccaac aggctccgca
     4261 ggctccgctc gccgtcggcg accaggcgtc gggcgacatc ggtccccgat ccggtccgga
     4321 cgaccgccgg caacggctcg ccacgaccgc gccgttcgag gatcacggca ccgaacgcct
     4381 cgtcggccgt gaccagggtc cggagcatca ggcggaggac gtcggcccgc aggctcggca
     4441 tgtcgccgcc gaccacgagg gcgatcggct ggtcgaccgc ctcgagcccg gccaggagcc
     4501 cgacgagcgg tccaccgtgc agctcgggat cggcgacgag ccggagcggc cgatcgatcg
     4561 gagccggcac gcgatcgtca cccggggcga cgaccaccac gatttcgacc gcgacggccg
     4621 cgagcgcggc cacggatcga tcgagcagcg tcgcgccctc gacgcgggcc gcgagcttgt
     4681 cggacccgta acggctcgat cgcccaccgg cgaggacgat cccgctggcg gcgacgtcca
     4741 gcggagcggt catcggggcg cgcccgcggg gctcgcggcg tccgccgcgg cgagctgcgg
     4801 cgcccgcggt cccggcacgt ggccggcggg ggtccgccgc aggatcgccc gtcgatcgag
     4861 gtcctcgagg agggccatga cgtacttccg gctcgttccc gtcgcgtcgc ggtaggcggc
     4921 gggcgtcagg gggccgccgc gggccagctc gatggcgcgc gcggccaggt cccgccaggc
     4981 cgggaacgcc caggccaggt cgtcgtccag ccggacgatc cggttcgcct gctcgaggag
     5041 gcggatccct tccggcggac agccggcggc gcgggcggcc gaggtcaacg gcggcgggct
     5101 gaccgtcgag agcgccgcga cgagacgatc catggcggtc gcgagcgccg gggacgggcc
     5161 gaccgcggcc acacccgctg gccggacgcg gtccgcgtcc cggctcagcc gatcggcgcc
     5221 gatgaggtcg tcgagccggc cggcaacgat tcgatgggcc gcatcgccgg cggcggcacc
     5281 gggaggtccg atcgtgccga cccggcggcg gagctccacc tcgcccgccc ggaccagctc
     5341 ggagatccgg atcccgggcc gtccctcgac cgcggccagg agcacgtcgt cgatggcggc
     5401 ggccacgtcc ggggcgatcc ccggcgagga gtcgagcacc acgccgtgca gctcggcgcg
     5461 agcgcttcgc cacgcgtccg agccggcgcc agccgtggcc agggcgcgca gccgctcgac
     5521 ggtcgttcga cgtctcgcga tgccgcgagc cggttgcgga tcgaggacgc gaccgccggc
     5581 cagggtctcg accggcgacg ggcggcggac gacgaagcga tcgcccggcg cgacggcgat
     5641 cgggcgatcg agccgcagga tcgccgatcc ttcgccgccg tcgaggtcga ccccgtcgcg
     5701 gccgctccgc ccgacgacgc cggccacccg tgcggttccg agatggacgg cgatccgtgt
     5761 ccggtctgcc ggcaggccgg ggccacggcc cgcggcggag gcggtccccg ctccgaacgc
     5821 ggtggcccgc cggagcgcga cgagccatcg gtccgacgcg acgaccctcg gatcggtcgt
     5881 caggacggcg ccccgcccga ggccgtcgac gccgtcgccg gcgacgttca gcgccacccg
     5941 tccggggccc gcccgatcga cggcccgacc gtggatctgg acctcgcgaa cccggatgct
     6001 ccgatcggcc gggccgggga cgacgcgcaa gagcgtaccg cgctcgatcg gcgtgccgcg
     6061 gagcgtcccg gtgacgaccg tcccccggcc gcggatcgag aaggcccgat cgatcgcgag
     6121 acggctgccc gcgtccggga tgtcgccggc cgatcggatc gcgatccgat cgcggaggtc
     6181 gaccagcgcc gtgacgagcg ccgcgagccc ctcgccggtc gtcgccgaaa ccgccaacac
     6241 ggggacaccg cccagggtcg tcgacgcgac gagagcgccg gcggccgcga cgacctcggc
     6301 gacgcgctcg ggaccggcga cgtcggactt ggtcacgacg atgagcccgt cgtcgatccc
     6361 gagcgcgtcg agcagctcga gatgctcgcg ggtctgggcc cgcggaccgt cgtcggccgc
     6421 gatcaccagc atcgcggcgt cgatctcgcc cgcgccaacg agcatgttgc cgacgagccg
     6481 gtcgtgcccg ggaacgtcga cgaagtcgag ctccgtcccg tcgggcaggg tcaggtgggc
     6541 g
//
LOCUS       DATAIW010000341         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_111394, whole genome shotgun sequence.
ACCESSION   DATAIW010000341 DATAIW010000000
VERSION     DATAIW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111394"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1130
                     /locus_tag="VHM48_10485"
     CDS             186..1130
                     /locus_tag="VHM48_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2755882.1"
                     /translation="MTAVAGAKTARPAAVARKRRSSRGRMSGRQQILLQILCLLITAT
                     VLFPILWIFSLALDPRNVSRPDGLIPPGASLAAFARVIERPTSDNIGLLELARNSFML
                     ASSVALFSVLIGVLSAYAFSRLRFRGREVLMIVILGVLMLPAVATIAPLFIMLNGVKL
                     DLPILGHVALRTSLIGVGLAVVSGTLPFAIWNLKGYLDTIPKELEEAAAVDGCTRNQT
                     FFKIVLPLATPALAVTGFLGFIAGWTEFYFVSIFLNDPSQGFTLSLALNKMVGAFGQT
                     PWSDFAAFSILFAAPVSIVFFFFQKYIVGGLAVGGVKG"
     gene            1308..1661
                     /locus_tag="VHM48_10490"
     CDS             1308..1661
                     /locus_tag="VHM48_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755883.1"
                     /translation="MPFPFLPGGVIVAATVLIGALTAFGLVLRAMDRAVLGLRDMAVS
                     SLVSGLRGWSNGRQASAGMESPRSTTSPWSVASRPESVPAPRAAPAGRPETFDPPEMI
                     DLGSRPIEEPVRRRR"
     gene            2003..>2337
                     /locus_tag="VHM48_10495"
     CDS             2003..>2337
                     /locus_tag="VHM48_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2755884.1"
                     /translation="MTAHLDALNDGDPATSDDLGIGGIWLMPIAASPSYHGYDVTDER
                     AVEPDYGTIDDLKALVAAAHARGIAVISDLVLNHTSDQNPWFTDSLAGTGHEDWYIWS
                     ATNPGYGGP"
BASE COUNT          309 a          852 c          788 g          388 t
ORIGIN      
        1 gctgttcgtg accgaggcgt acaagctcgt caacgacttc cagctgtacg gcgtcgcggc
       61 ggccttctcg gtgatcctct tcttcctgct tctcgggatc accctggtca ccaaccggtt
      121 ggcacgggcg accgcgagct attccgaatg aacaggctca gccacgacgc ccgagggagg
      181 aagcgatgac cgccgttgcc ggcgccaaga cggcccgtcc cgcggccgtg gcgcggaagc
      241 gaagatccag ccgtggacgg atgagtggca ggcagcagat cctgctccag atcctgtgcc
      301 tgctgatcac cgcgaccgtg ctcttcccga ttctctggat cttctcgctg gcgctcgatc
      361 cacggaacgt ttcccggccg gacggcctga tcccgcccgg cgcctcgctg gccgcattcg
      421 cccgggtgat cgagaggccc accagcgaca acatcggcct gctggagctg gcccgcaaca
      481 gcttcatgct cgcgagcagc gtggcgctct tctcggtcct gatcggcgtc ctgtccgcct
      541 acgccttctc ccgcctccgg ttccgcggcc gggaggtcct gatgatcgtg atcctcgggg
      601 tgctgatgct gccggccgtc gccacgatcg cccccctgtt catcatgctc aacggggtga
      661 agctggacct gccgatcctc ggtcacgtgg cgctccggac gtcgctcatc ggggtcgggc
      721 tggcggtcgt gtcggggacg ctgccgttcg cgatctggaa cctcaagggc tatctcgaca
      781 cgatccccaa ggagctcgag gaagcggcgg ccgtcgacgg ctgcacccgc aaccagacgt
      841 tcttcaagat cgtcctgccg ctcgccacgc ccgccctggc ggtcaccggc ttcctcggct
      901 tcatcgccgg ctggaccgag ttctacttcg tctcgatctt cctcaacgac ccgagccagg
      961 ggttcaccct gtcgctcgcc ctcaacaaga tggtcggggc gttcgggcag acgccgtggt
     1021 cggacttcgc cgccttctcg atcctgttcg cggcaccggt ctcgatcgtg ttcttcttct
     1081 tccagaagta catcgtcggc ggcctggcgg tcggcggggt caagggctga gcggatcgtc
     1141 gcccggcggg acggccggga cggccgggac accacgcgac gggcgctccg ccgcgggcca
     1201 tgaggacctt cagcactcga cagcgagcgt cgggcgggcg atcatcgggc cagcgactga
     1261 cgatccaccg acgtcggtgg atcgggtcgt cccagaggga ggtcgacatg cccttcccgt
     1321 tcctgccggg tggcgtcatc gtggcggcga ccgtgctgat cggcgcgctg acggcattcg
     1381 ggctcgtgct ccgggcgatg gaccgggcgg tcctcgggct ccgcgacatg gccgtgtcga
     1441 gcctcgtgtc cggcctgcgc gggtggagca acggccgtca ggcctcggcg ggcatggagt
     1501 caccgcgctc gacgacgagc ccgtggtccg tcgcgagtcg acccgaatcc gtgccggctc
     1561 cccgggccgc gccggcgggc cgccccgaga ccttcgatcc gcccgagatg atcgacctcg
     1621 gcagccgtcc gatcgaggag cccgttcgcc gtcgccgctg acagtcgacg ccccgtcggg
     1681 acgccctctc cggtcggcgg cgcggtcccg gcttgtcggc gtcgtcgcca tcgtcgccgc
     1741 gctcgtcgca gcctgctcgc cggcgcccgg ctcaccgccg ccgtccgtcg gcccgagcgg
     1801 cggcgcgtcg agcgcgagtg cgtcggccgg ggcatcggcc gctgccggcg ccccgccgag
     1861 ctgcccgccg gtttccgcca cgagcgcgac cgcgcctgca ggactcggtc agcccggctg
     1921 gtggcacggc cgcgtcttct acgagatctt cgtccggagc ttcgccgact ccgacggtga
     1981 tgggatcggc gacctgcgcg gcctgacggc ccatctcgac gccctcaacg atggcgatcc
     2041 tgcgacgagc gacgacctcg gcatcggcgg gatctggctg atgccgatcg ccgcatcgcc
     2101 gagctaccac ggctacgacg tcaccgacga gcgggcggtc gagccagact acggcacgat
     2161 cgatgacctc aaggcgctcg tcgcggcggc gcacgcgcgg gggatcgcgg tgatcagcga
     2221 cctcgtcctc aaccacacgt ccgaccagaa cccgtggttc accgattcgc tggccgggac
     2281 cggccacgag gactggtaca tctggtcggc cacgaacccg ggctacggcg ggcccga
//
LOCUS       DATAIW010000342         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_51705, whole genome shotgun sequence.
ACCESSION   DATAIW010000342 DATAIW010000000
VERSION     DATAIW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51705"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..749)
                     /locus_tag="VHM48_10500"
     CDS             complement(384..749)
                     /locus_tag="VHM48_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755885.1"
                     /translation="MLVALTASRPPWLTLIVSPDTFRGNSTGSVVGAVGGIAAEADAD
                     SADGADGAAAGADLAAAGFALEARPGRLPLVRALGVIVLRAASSAASRAASASAPARV
                     SSLVGSGVSRSAIGSFSHA"
     gene            754..1122
                     /locus_tag="VHM48_10505"
     CDS             754..1122
                     /locus_tag="VHM48_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755886.1"
                     /translation="MHQGGIGRARATDIAVTQGGVGAARADRVSVELGGIGAALTGEL
                     TITQGAAGTVVARDARIEQSIVRTLVANVVHFERPSGVLFLIARKVDGNVKVLLDWRG
                     AVAFGAAFGVITSLFRRRRR"
     gene            1217..1888
                     /locus_tag="VHM48_10510"
     CDS             1217..1888
                     /locus_tag="VHM48_10510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2755887.1"
                     /translation="MIDPSARVHATADIEPDVQIASGTSVDQRTRIGGGARLGADCSV
                     GRDVFIDAAVVIGDRVQVHHRAMIYEGVGIADSVFIGPGAILTNDRYPRAVASTGDLA
                     RAADRPIVPSTLAEGCTIGAGAVVVAGVAIGSWAMVGAGAVVTRDVPNHALVVGSPAH
                     RIGWVCACGARLVDSKGDPMAAEPGRYAAHQELICPSCGRTYAYIADAETLDERSGPR
                     QGAPA"
     gene            complement(1912..3240)
                     /locus_tag="VHM48_10515"
     CDS             complement(1912..3240)
                     /locus_tag="VHM48_10515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX2755888.1"
                     /translation="MHLFLIGAGHVGLVTAVGLARLGHRLTVADIDETRIAGLAKGVP
                     PVFEPGLEEAIAQAAAAGLLTFTTDLTPPTDCAFSIVTVSTPTGPDGPLSTANVERVV
                     ATLVEKTGPEHTIVVRSTLPVDGPARLLAIGATRPDRPAIVTNPEFMREGSALADFDR
                     PDRLVVGWIEPRDEAAAKAVLDLYAGIVAPTLVADARSVALIKLATNVFLAAKVAYAN
                     ELARICDVIGADVDTVADGIGLDPRIGRSFLTAGPGYGGSCLPEQAIALSLITAARGI
                     PTPLIEAVSRSNETHQRAIVARLGALLGNGDDRAPAGGSGRGLAGARIALLGLAFKAN
                     TDDVRESPALSLARYVREAGATVVGTDPRAIAKARRADPELMTADTVVDAVAGADAVL
                     VATEWPEFADLDWTDLAARMAGELVYDTRDILPADRVMAAGLRYVSLGRG"
     gene            3390..>4176
                     /locus_tag="VHM48_10520"
     CDS             3390..>4176
                     /locus_tag="VHM48_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755889.1"
                     /translation="MKVAIVAANTFQYDARQLRSAAALTSDGHTVTLVGLAGPGLPTR
                     ERLDGGIELRRVAVDGTIASAFRPLPPAGRRAVARLLGIDPTATALPPGRPRGLDRLR
                     APFRRVAEILAHARRVGPWSAAVVAAVPDADVFACKALIALPVIRAAARRTGGRFVYD
                     IADIHTEAARLARMPGWFRALVRGRERRWMREAAGLTAVSPDVADEVVRLFGVARPTV
                     VLNCPPAWRPGEVLPAPTGRLRALAIVPSTATRRSSIPPSSRSR"
BASE COUNT          549 a         1573 c         1520 g          534 t
ORIGIN      
        1 ctcgatggcg gcgtgctgcc gagcccggcg aggaggccgg agtcagccgc gctggagcct
       61 ggccgcccgg aggcgagggc ggcgaacatc ccgaggccga gaccaccgag gatcgcgagg
      121 attccgagcg cgacgacggc agcgcgactc atcggacggt gagcgggtcg atcatgcgtt
      181 gagcccctcg aaacggccgc cgtgacggac gcgatccgga ccggtgtcgg cgcgcctcgc
      241 ggcgggacgg tgacggcgtc gcgggcgcag catacgctgc ggccgcctgc cgggcactcg
      301 acgggtcggc cggccggttc gtgacggcgc cggtggagca cggtacgctg acagccgacg
      361 tcccgccggg caacccggac cgatcacgcg tgggagaacg aaccgatggc cgagcgagac
      421 acccccgagc cgacgagcga cgacaccctc gccggtgccg aggccgacgc tgctcgcgag
      481 gcggccgacg aagcggcgcg caagacgatc acacccaggg cccgcaccag cggcaagcgt
      541 cccggccggg cctcgagcgc gaagcccgcg gccgccaggt ctgcgccggc ggccgcgcca
      601 tccgcgccat ccgcgctatc cgcgtcggcc tccgccgcga tgccgcccac cgcgccgacg
      661 acgctccccg tcgagttgcc acggaacgtg tcgggcgaga cgatcagcgt gagccagggc
      721 ggcctcgacg cggtgagcgc cacgagcatc gacgtgcatc agggcggcat cggccgcgcc
      781 cgggcaaccg acatcgccgt cacccagggc ggcgtcggcg cggctcgggc ggaccgcgtc
      841 tcggtcgagc tcggcgggat cggcgcagcg ctgaccggcg agctgacgat cacccagggc
      901 gcggccggga cggtcgttgc ccgcgacgcc cggatcgagc agtcgatcgt ccgcacgctc
      961 gtcgcaaacg tggtccattt cgaacgcccg agcggcgtcc tgttcctcat cgcccgcaag
     1021 gtcgacggca acgtcaaggt gctcctcgac tggcgcggtg ccgttgcctt cggggccgcg
     1081 ttcggggtca tcacgtcgct gttccgccgc cgccgccgct aggaccggtt tccgggtcgc
     1141 cctcggctat actcgcggcc cgatccgacc ccgagccgcc cggcggcggc catccacctg
     1201 gagccttcga ctgcccgtga tcgatccttc tgcgcgcgtc cacgcgaccg ccgacatcga
     1261 acctgacgtc cagatcgcgt cgggcacgag cgtcgaccag cgaacgagga tcggtggcgg
     1321 cgcccgcctc ggtgccgact gcagcgtcgg ccgcgacgtg ttcatcgatg ccgccgtcgt
     1381 gatcggcgac cgggtccagg tccaccatcg ggcgatgatc tatgagggcg tcggcatcgc
     1441 cgacagcgtg ttcatcgggc caggcgcgat cctgaccaac gaccgctacc cgcgcgccgt
     1501 cgcctccacc ggcgacctcg cccgggccgc cgaccgaccg atcgtcccga gcacgctggc
     1561 cgagggttgc acgatcggtg ccggcgcggt cgtcgtcgcc ggcgtggcga tcgggtcctg
     1621 ggcgatggtc ggagccgggg cggtcgtcac ccgcgacgtc ccgaaccacg cgctcgtcgt
     1681 cggcagcccg gcccaccgga tcggctgggt ctgcgcctgc ggcgcgcgtc tcgtcgacag
     1741 caagggcgat ccgatggccg ccgaacccgg ccgctacgcc gcccaccagg agctcatctg
     1801 cccgtcatgc ggccggacgt acgcctacat cgccgacgcc gagacgctcg atgagcggag
     1861 tggaccgagg cagggcgccc ccgcctgatc gccggccctt tccgggccgc gtcagccccg
     1921 cccgagcgac acgtagcgca ggccggcggc catgacccgg tcggccggaa ggatgtcccg
     1981 ggtgtcatac accagttcgc ctgccatccg cgccgcgaga tcggtccagt cgagatccgc
     2041 gaactcgggc cattcggtcg cgaccagcac ggcgtcggcg cccgcgaccg cgtcgacgac
     2101 ggtgtcggcc gtcatcagct ccgggtccgc tcgccgagcc ttggcgatcg cccgcgggtc
     2161 ggtcccgacg acggtggcac cggcctcgcg aacgtagcga gcgagcgaca gggccggcga
     2221 ttcacggacg tcgtcggtgt tcgccttgaa cgccaggccg agcagcgcga tccgggcgcc
     2281 ggcgagaccg cggcccgagc cgccggccgg ggcgcggtcg tcgccgttcc cgaggagcgc
     2341 ccccagccgc gccacgatcg cccgctggtg cgtctcgttc gaccggctga ccgcctcgat
     2401 cagcggggtc gggatcccgc gcgccgccgt gatcagcgac agggcgatcg cctgctcggg
     2461 gaggcacgag ccgccatagc ccgggccggc ggtcaggaac gaacgaccga tccgcgggtc
     2521 gagcccgatc ccgtcggcga cggtgtcgac gtccgccccg atgacgtcgc agatccgggc
     2581 gagctcgttg gcataggcaa ccttggcggc gaggaacacg ttggtggcga gcttgatgag
     2641 ggcgaccgaa cgcgcgtccg cgacgagggt cggcgcaacg atcccggcgt agaggtcgag
     2701 cacggccttc gccgcggcct cgtcgcgcgg ctcgatccag ccgacgacca ggcggtccgg
     2761 tcgatcgaag tcggcgaggg cagaaccctc gcgcatgaat tccgggttcg tgacgatcgc
     2821 cggccggtcc ggccgggtcg ccccgatcgc gaggaggcgc gccgggccgt cgacggggag
     2881 cgtcgagcgg acgacgatcg tgtgctcggg gccggtcttc tcgacgagcg tcgccacgac
     2941 ccgctcgacg ttggcggtcg agagcggccc gtcgggcccg gtcggcgtcg agaccgtcac
     3001 gatcgagaat gcgcaatcgg tcggcggggt cagatcggtc gtgaaggtga gcagaccggc
     3061 ggcggcagcc tgggcgatcg cctcctcgag gccaggctcg aacacgggcg ggacgccctt
     3121 ggcgaggccg gcgatccggg tctcgtcgat gtcggcgacg gtcaggcgat gaccgagccg
     3181 ggcgagtccg acggcggtca cgaggccgac gtggcccgcg ccgatcagga acagatgcat
     3241 gggtgctccc gggacatggg tgggagtccc attctatcgt cggccggacg gcgaccggcc
     3301 gggcggaccg gccggggcga ctcgggtacg cttcggcgac caatgacccc tccgctgtcc
     3361 gcatccggct cctccgtcac gctcgtccgg tgaaggtcgc gatcgtcgcc gcgaacacgt
     3421 tccagtacga tgcccgccag ctccgctccg ctgccgcgct gacctccgac ggccacaccg
     3481 tcaccctcgt cgggctcgcc ggcccgggcc tgccgacacg cgagcggctc gatgggggga
     3541 tcgagctgcg ccgggtggct gtcgacggaa cgatcgcgag cgcgttccgg ccgcttccac
     3601 ccgccggccg tcgcgcggtc gcccggttgc tcggcatcga cccgaccgcg accgccctgc
     3661 cgccgggccg tccacgcggt ctcgaccgcc tccgggctcc gttccgccgc gtcgccgaga
     3721 tcctcgccca tgcccgccgc gtcgggccgt ggagcgcggc cgtcgtcgcg gccgtccccg
     3781 acgccgacgt cttcgcctgc aaggccctga tcgccctgcc cgtcatccgc gccgcggccc
     3841 gtcggacggg cggccggttc gtctacgaca tcgccgacat ccacaccgaa gccgctcgcc
     3901 tggcgcggat gccgggctgg ttccgggccc tcgtccgcgg ccgcgagcga cgctggatgc
     3961 gggaggccgc cgggctcact gcggtcagcc cggacgtcgc cgacgaggtc gtccgcctgt
     4021 tcggggtcgc ccgcccgacc gtcgtcctga actgtccgcc ggcgtggcgg cccggcgagg
     4081 tcctgcccgc cccgaccggg cggctgcgag cgctcgcgat cgttccgtcg actgccaccc
     4141 ggcgcagctc gatcccccca tcgagccgct cgcgtg
//
LOCUS       DATAIW010000343         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_171215, whole genome shotgun sequence.
ACCESSION   DATAIW010000343 DATAIW010000000
VERSION     DATAIW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171215"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VHM48_10525"
     CDS             <1..585
                     /locus_tag="VHM48_10525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2755890.1"
                     /translation="GMESGSQIVGVVARLEAEKGHPTLLDAWPGVLRAVPDAYLLIVG
                     EGSRRDALEAQARELRIAHRIVFTGRRDDVPAVTAALDVAVLPSYREAQGLTILEAMA
                     LSRPVVASNVGGIPEMVEDGVTGILVPPHDAEALTTAIVRLLTDHPYADMLGRAGHDL
                     VHDRFCIELMVQAVESIYDEGAGIVRPSEVAAAV"
     gene            complement(643..1074)
                     /locus_tag="VHM48_10530"
     CDS             complement(643..1074)
                     /locus_tag="VHM48_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755891.1"
                     /translation="MSPRRIGRTATCDRADATLRLRHAESFLVVADLVLDQSDDPALA
                     LTSVAASLAVLAGIAAADAACCVALGRRARGQNHEEAVALVRTIAPNGEAMARDLSRL
                     LALKDDAQYGVLIVSSERARSSVGWARRLLEATREVVAPAG"
     gene            complement(1071..>1513)
                     /locus_tag="VHM48_10535"
     CDS             complement(1071..>1513)
                     /locus_tag="VHM48_10535"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX2755892.1"
                     /translation="ATLYVANRSHLAWPMLEALVGLRSGLLGRIREVVAGWEVAPLHV
                     SLFGSAARRDGGAESDIDMLIVRPDDVAEGDETWETQIDTLRDQVVAWTGNRCQAFDI
                     GRARLREHLAAHDPLVENWLRDEVLLAGEPLRSLIDNLAVVARS"
BASE COUNT          223 a          526 c          550 g          214 t
ORIGIN      
        1 gggatggagt ccgggtcgca gatcgtcggt gtcgtcgcgc ggctggaagc ggagaagggc
       61 cacccgacgc tgctcgacgc gtggcccggc gtcctgcgcg ccgtccccga cgcctacctg
      121 ctgatcgtcg gcgaggggag ccgccgcgac gccctcgagg cgcaggcgcg cgagttgcgg
      181 atcgcccatc ggatcgtctt taccggacgc cgtgacgacg tgccggccgt gaccgcggcc
      241 ctcgacgtcg cggtcctgcc gtcctatcgc gaggcgcagg ggctgaccat cctggaggcg
      301 atggcactct caaggccggt cgtcgcgtcc aacgtcggcg gcatcccgga gatggtcgag
      361 gacggcgtca ccgggatcct ggtcccgccc cacgacgccg aggcgttgac gaccgccatc
      421 gtccggctgc tgaccgacca cccgtatgcc gacatgctcg gccgggcggg ccacgacctg
      481 gtccacgacc gcttctgcat cgaattgatg gtccaggccg tcgagtccat ctacgacgag
      541 ggcgccggta tcgtgcgccc gagcgaggtc gccgccgccg tctgagcgac gggccgtcgc
      601 ctgcgcgtcg ccctgcggcc actggacagc cgtgggcctg cgtcagccgg ccggtgcgac
      661 cacctcacgc gtcgcctcga ggagccgacg cgcccaaccg accgaggatc gggcacgctc
      721 ggacgagacg atgaggactc cgtactgagc gtcgtccttg agcgcgagga gacggctgag
      781 atcgcgagcc atcgcctcgc cgttcggcgc aatggtgcgg acgagcgcga cggcctcttc
      841 atggttctgc ccgcgggcgc gacggccgag cgcgacgcag cacgcggcgt cggccgcggc
      901 gatccctgcg aggacggcga gcgacgccgc cacgcttgtg agtgcgagag ccgggtcgtc
      961 cgactggtca agaacgagat cggcgacgac gaggaacgac tccgcgtggc ggagccggag
     1021 ggtcgcgtcg gcgcggtcgc acgtcgccgt tcggccaatt cggcgaggac tcatgacctg
     1081 gccacgacag cgaggttgtc gatgagcgag cggagtggct cgcccgcgag gaggacctcg
     1141 tcgcggagcc agttctcgac gagcggatcg tgggccgcga ggtgctcacg gagccgggct
     1201 cggccgatgt cgaacgcctg gcaccggttg cccgtccagg ccacgacctg gtcgcggagg
     1261 gtatcgatct gcgtctccca ggtctcgtct ccctccgcca cgtcgtcagg gcggacgatc
     1321 agcatgtcaa tgtcgctctc cgcgcctccg tcgcgtcgcg cggcactacc gaacagcgac
     1381 acgtggaggg gggcgacctc ccagccggcg acgacctcgc ggatacgccc caggagacca
     1441 cttcggaggc cgacgagagc ctcgagcatc ggccatgcga ggtgtgatcg gttcgcaacg
     1501 tacagggtcg cgt
//
LOCUS       DATAIW010000344         8484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_248025, whole genome shotgun sequence.
ACCESSION   DATAIW010000344 DATAIW010000000
VERSION     DATAIW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248025"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VHM48_10540"
     CDS             <1..893
                     /locus_tag="VHM48_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2755893.1"
                     /translation="PTHPYLYVNFTDRNGNTAIDRFTVSANPNVASHASGLRLLTIAQ
                     PYSNHNGGNLAFGPDGYLYIGMGDGGSAGDPGNRAQNINSPLGKMLRIDVNHGSGTRH
                     YRSPATNPYVGRTGLDEIWSRGLRNPWRWSFDRLTGQLWIGDVGQSRWEEVDRSSAHG
                     TTPAGRAVNYGWNVLEGRACYKPATGCSTSGKQPPQTVYGHAVSGTTNCAVTGGFVYR
                     GSAYPVLAGGYVFGDYCSGRIWVMSPLATAPVTATLVRDATAGPHLAISSFGEDEAGE
                     LYVCDLAGGAIYRVTASARP"
     gene            complement(1005..2297)
                     /locus_tag="VHM48_10545"
     CDS             complement(1005..2297)
                     /locus_tag="VHM48_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2755894.1"
                     /translation="MNRSSVDPPATAPDDDAPDDDAGPAGTAPTGALAAFMPAGPHED
                     AKARIAAAVDAARTEIIELSHRIHAHPEPAYEEVQAATWVATVLRGHGYAVEHPAGSL
                     ATAIRATRAGGRGGAGPRIGILAEYDALPGLGHGCGHNTMAASGVGAAIALAAVADEL
                     PGEIVFLGTPAEERGSGKKTMIDDGLFDGVDAALLFHPCDRNHVESQPLASEDVDVVF
                     HGLQAHAAADPWRGRNALDAMILLFSSVGLWRQQLRTEARVHGIIQEGGTAANIIPER
                     TRAWFMLRSPDQAYYEVMKTRFRSLVDAAALATETTVEISFSGGAMTMKPNATLEARW
                     VANAAAYGIVDQGPDGNSGSTDMGNVSWVCPTIHPELSIAPEGTPGHSILFRDAAVTP
                     AADATTLLAATLVAQTAYELFADPELVAAAWRDFRGEG"
     gene            complement(2335..2826)
                     /locus_tag="VHM48_10550"
     CDS             complement(2335..2826)
                     /locus_tag="VHM48_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755895.1"
                     /translation="MTAEDASLTLGFDEPDLGANDGEPGRWPGIAWNTLDSLVMEARD
                     RLTNLGFFAGAGVVWILVALVVTTRDPRLDPAAGFVGALLIGLAIGLTVVPLFWLAIF
                     ARHRRIAYRGDWTRAVRRGAWVAVVTAVFVILRLQGAFVPALALFILALVVLAEAVLS
                     VER"
     gene            complement(2823..3227)
                     /locus_tag="VHM48_10555"
     CDS             complement(2823..3227)
                     /locus_tag="VHM48_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2755896.1"
                     /translation="MQTTSDPVGGLAVLVVDDEPLIRAYVARALTLAGFEVAVAASGR
                     EALRLVADGRVRPAVVVTDIEMPEMTGVELAARLLALRPTLRIVMMTGDLARAEAARG
                     HPSIVDEVLVKPMRTEDLVNAVRPTPDAARIP"
     gene            complement(3368..4213)
                     /locus_tag="VHM48_10560"
     CDS             complement(3368..4213)
                     /locus_tag="VHM48_10560"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2755897.1"
                     /translation="MTKRLAGITAITFDFGNTLVPVSRSALRRVVELTADAICERWPD
                     LERTAFLAAWAEERERQFREEVPQFREVDLAERLIRVFARLRGMDPPGPDERWDQSRA
                     AEHADGAAVEWGVEQYSRAFVDGLPASPAVAAMLGRLAADRDLAILSNWPLAATIDRF
                     AEAHGWSPHLRAIVVSQRVGTIKPQPAIFEAARTAMGNPDPGSILHVGDDWAADIVGA
                     RAVGWRTAWLLARSDESPLPISERDGSRDPDLELASLDELESHLAAPDSSSANGGDGD
                     RVAAR"
     gene            complement(4210..7371)
                     /locus_tag="VHM48_10565"
     CDS             complement(4210..7371)
                     /locus_tag="VHM48_10565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2755898.1"
                     /translation="MAPDPTALDDTMSRPRTRREPVPLEQIPVWDGFLADGPTTPSGD
                     PPAGLDSLLGGLNPEQLKAVTHGDGPLLVVAGAGTGKTQVITRRIAWLIATRRARPSE
                     ILALTFTEKAAAEMQVRVDQLVPYGYTDTTIGTFHAFGDRLIREYALELGLPSDIRVL
                     SRAETVIFLREHLFAFELERYRPLGDPTRFLAALATLFSRCKDEDVSPEAYAAFADGL
                     ASAAAAAPDDEVLAEEAGRQRELARAYARYQELLETNGCIDFGDQVALALRLVRTSPA
                     ARAEIQARFRYVLVDEFQDTNRAQSELVSLVADRHRNLTVVGDDDQSIYRFRGAAISN
                     ILGFRDRYRNARTVVLRRNYRSRPAILEAAHRLVRFNDPDRLEVQAGISKRLVAERGI
                     DPAGRTVRLETHPSGHDEADRIATEIAGRISTGTAPRDIAILVRANGHADPILRSLDA
                     AAVPWRFSGTSGLYARPEVRRLLAFLRAIADLGSSVDVYGVATGEPYHLGGEDLTAIV
                     NSARRRNRTLWEVIEEIDRQPGILRISAASRTAVARLVTDLRELSTIAHERPAGEVLY
                     AFLRRSGTLSRLAAEATVGAEEQLRNVARFFDIVRSQSALLADDRATFVAGHLQTLIE
                     AGDDPATADLDPDSDAVAVLTVHKAKGLEFPIVYLPGLVAGRFPIPNRRDALALPAVL
                     GDVVDGPDEEVQLREERRLFYVAMTRARDELILSHAVDYGGVGVRRLSPFVLEALDLP
                     PTAVDPVVRAANAIDRIEAANLTSTPVRPSTGRGPTGEPLTLSFSGIDAWLSCPLKYK
                     LGHVVRVPVPPHHAMIYGSALHRAVQEFHRRHARGEVMTEDELIAALEAAWTNEGFLS
                     RDHEDARLAAGRETLRRFRAAQLVPGAVIPAYVEREFSFALGGDRIRGRWDRVDIEPA
                     GDPQADVDAGDRPDPSADTVAPTLELTGREQVTITDYKTSDVRDPVKARQRARESLQL
                     QIYAMGYEALTGRLPDAVQLHFLDTGVTGRVAVDPKRLARGRQKIAAAAAGIRARDFS
                     PKPDRSTCGYCPFKTICPSSVAV"
     gene            complement(7409..7957)
                     /locus_tag="VHM48_10570"
     CDS             complement(7409..7957)
                     /locus_tag="VHM48_10570"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX2755899.1"
                     /translation="MDLNAFVAPFVGAAIVGLAVVLLGLLLLTIVQTRRIGRLRSRLD
                     SLTRGADGASLGEVLDTHLDKVFAVARELDELSARSAVLEATGRRTIQRVGLVRFNPF
                     EDTGGNQSFALALTDAAGNGFIVSSLHSRTGTRVYAKAVSAGRSDGALSQEETEALRL
                     AQAGQAAVARDQHVRDRVAGPA"
     gene            8278..>8484
                     /locus_tag="VHM48_10575"
     CDS             8278..>8484
                     /locus_tag="VHM48_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755900.1"
                     /translation="MTRAEEARAAELEAAILAEEKAADASRQTRERARRPSVESVGGV
                     SYTSIPLSVRAADEYGYVKRDIRRI"
BASE COUNT         1251 a         2973 c         3079 g         1181 t
ORIGIN      
        1 acccgaccca cccctatctg tacgtgaact tcaccgatcg caacgggaac acggcgatcg
       61 accgtttcac ggtcagcgcc aaccccaacg tggcaagcca tgccagcggc ctgcggctcc
      121 tgacgatcgc ccagccgtac agcaaccaca acggcgggaa cctcgcattc ggtccggacg
      181 gttacctgta catcgggatg ggtgacggcg gcagcgccgg cgatccgggc aaccgcgccc
      241 agaacatcaa ctcgccgctc ggcaagatgc tccggatcga tgtcaatcac ggatccggga
      301 cgcgccacta ccggtccccg gcgaccaacc catacgtcgg gcggaccggc ctcgacgaga
      361 tctggtcgcg cgggctccgc aatccgtggc gctggtcatt cgatcgcctg acgggccagc
      421 tctggatcgg cgatgtcggc cagagtcgct gggaggaggt cgaccgatcg agcgcgcacg
      481 gcacgacccc ggccgggcgg gccgtcaact acggctggaa cgtcctcgag ggtcgagctt
      541 gctacaagcc cgccaccggc tgttcgacca gcggcaagca gccgccgcag actgtctatg
      601 gccacgccgt cagcggcacc accaactgcg cggtcaccgg cggcttcgtc tatcgcggct
      661 cggcctatcc cgtcctcgcc ggcggatatg tcttcggcga ctactgctcg ggacggatct
      721 gggtgatgtc gccgctggcc acggcgccgg tgacggcgac cctcgtccgc gatgccacgg
      781 cgggcccgca cctggcgatc agctcgttcg gtgaggacga ggcgggcgag ctgtatgtct
      841 gcgacctggc cggcggcgcg atctaccggg tcaccgccag cgccaggccc tgaggcgccg
      901 ccgtcgagtg gcgcggtcga ggggcgcggt cgaggggcgc ggtcgagggg cgccggcccg
      961 gccgacgccg gcctgctcgt gccgggtcga gcccgggccg ggaatcagcc ctcgccccgg
     1021 aagtcgcgcc acgcggctgc cacgagttcc ggatcggcga agagctcgta ggccgtctgg
     1081 gcgacgaggg tggcggcgag cagtgtcgtg gcgtccgcgg ccggcgtcac agcggcgtcg
     1141 cggaacagga tcgagtggcc tggcgtgccc tccggcgcga tcgacagctc ggggtggatc
     1201 gtcgggcaga cccaactgac gttgcccatg tccgtgctcc cggagttgcc atccggaccc
     1261 tggtcgacga tcccgtaggc cgcggcgttg gccacccacc gggcttcgag cgtggcgttc
     1321 ggcttcatgg tcatcgcgcc accgctgaag gagatctcga cggtggtctc ggtcgcgagc
     1381 gccgccgcat cgacgaggga gcggaagcgg gtcttcatca cctcgtagta ggcctggtcc
     1441 gggctgcgca gcatgaacca cgcccgggtc cgctcgggga tgatgttggc cgccgtcccg
     1501 ccctcctgga tgatcccgtg gacacgggct tcggtccgga gttgctgacg ccacagcccg
     1561 accgagctga acagcaggat catcgcgtcg agggcgttcc tgccccgcca gggatcggcc
     1621 gcggcatggg cctgcaggcc gtggaagacg acgtcgacat cctcggatgc gaggggctgc
     1681 gactcgacgt gattgcggtc gcacggatgg aagagcaggg cggcgtcgac accgtcgaac
     1741 aggccgtcat cgatcatcgt cttcttgccg ctcccccgtt cctcggccgg cgtgccgagg
     1801 aacacgatct cgccgggcag ctcgtcggcg acggctgcga gggcgatcgc ggcgccgaca
     1861 ccggacgcgg ccatcgtgtt gtgaccgcag ccgtgtccga ggcccggaag cgcgtcgtat
     1921 tcggccagga tgccgatccg cggaccggca cctccccgcc cgccggcgcg ggtggcccgg
     1981 atcgcggtgg cgagcgatcc cgccgggtgc tcgacggcgt atccgtggcc gcgcagaacc
     2041 gtggcgaccc aggtggcggc ctggacctcc tcgtatgccg gttcggggtg ggcgtggatg
     2101 cggtgcgaga gctcgatgat ctcggtccgc gcggcgtcga cggcggccgc gatccgagcc
     2161 ttggcgtctt cgtgcgggcc ggccggcatg aacgcggcca gggcgcccgt cggggccgtc
     2221 cccgcggggc cggcatcgtc atccggggca tcgtcatccg gggcggtggc aggcgggtcg
     2281 acagaggaac gattcacgcg acctcccttg gggcagccag gccgctcctc gtggtcagcg
     2341 ctccacggac aggacggcct cggcgaggac gacgagggcc aggatgaaca gggcgagggc
     2401 cgggacgaag gccccttgca ggcggaggat gacgaacacc gcggtcacga cagcaaccca
     2461 ggcaccacgt ctgacggccc gggtccagtc gccacgatag gcgatccgtc ggtgtcgggc
     2521 gaagatcgcg agccagaaca ggggcacgac cgtcaggccg atcgccagtc cgatcaggag
     2581 cgcaccgacg aacccggcgg ccggatcgag ccgcgggtcc cgcgtggtca cgacgagagc
     2641 cacgaggatc cacacgacgc cggcgccggc gaagaacccg agattcgtca gtcgatcgcg
     2701 cgcttccatc acgagggagt ctagggtgtt ccacgcgatg cccggccagc gcccgggctc
     2761 cccgtcgttc gcgccaaggt ccggttcatc gaacccgaga gtgagcgatg cgtcctccgc
     2821 ggtcacggga tgcgtgccgc gtcgggcgtc ggccgcaccg cgttcacgag gtcctcggtc
     2881 cgcatcggct tgacgaggac ctcgtcgacg atcgacggat gaccgcgcgc ggcttcggcc
     2941 cgggccaggt cgccggtcat catcacgatt cggagcgtcg gcctcagggc gaggagccgg
     3001 gcggcgagtt cgacgccggt catttcgggc atctcgatat ccgtgacgac gacggccggt
     3061 cggacccgcc catcggcgac gagccgcagc gcttcccgcc cgctcgcggc cacggcgacc
     3121 tcgaaaccgg ccagggtgag ggcgcgggcg acgtaggccc ggatcagcgg ctcgtcatcg
     3181 acgacgagca cggcgaggcc gccgaccggg tcggacgttg tctgcacacc cgcaggtcgc
     3241 tgcgtcgagc cgccgcccat cggcgatctg gtcctccgtg cccgagaggg tcgtcgattc
     3301 tgccggccga cgtggctggc ggtgcatgct cgcaccgcgg ccgggggtcg ccaatcgggc
     3361 gaatgcccta tcgggcggcg acccggtccc cgtcgccgcc gttcgcgctc gaactgtccg
     3421 gtgccgcgag atgcgactcg agctcgtcga gcgatgccag ctcgaggtcc ggatcgcgcg
     3481 acccgtcgcg ctcgctgatc ggaaggggcg actcgtccga tcgtgcgagg agccaggccg
     3541 ttcgccatcc gacggccctc gcaccgacga tgtcggcagc ccagtcgtcg ccgacatgca
     3601 ggatcgaccc gggatccggg ttgcccattg ccgtccgcgc cgcctcgaag atcgccggct
     3661 gtggcttgat cgtcccgacc cgctggctga cgacgatcgc tcgaaggtgt ggcgaccagc
     3721 cgtgggcctc ggcgaagcgg tcgatggttg ccgccagcgg ccagttcgac aggatggcca
     3781 ggtcgcgatc ggcggccaat cgaccgagca tggcggcgac cgcgggcgac gccggcaacc
     3841 cgtcgacgaa ggcccggctg tattgctcga cgccccattc cacggccgcg ccatccgcat
     3901 gctcggcggc ccgggattgg tcccagcgct cgtccgggcc cggcggatcc atgccacgta
     3961 gcctggcgaa cacgcggatc agtcgttcgg cgaggtcgac ctcgcggaac tgcggcacct
     4021 cctcgcggaa ctgccgctcc cgctcctcgg cccaggccgc caggaaggcg gtccgctcga
     4081 ggtcaggcca tcgctcgcag atcgcgtcgg ccgtgagctc gacgactcga cggagggccg
     4141 atcggctgac cggcacgagg gtattgccga agtcgaaggt gatcgcggtg atccccgcga
     4201 gccgcttcgt cacaccgcca cgctggacgg gcagatcgtc ttgaacgggc agtaaccaca
     4261 ggtcgaccgg tcgggcttcg ggctgaagtc gcgggcgcgg atcccggccg ccgcggctgc
     4321 gatcttctgc cgacccctgg ccaggcgctt cgggtcgacc gcgacccgcc cggtcacgcc
     4381 cgtatcgagg aagtgcagct ggaccgcatc gggcaggcgg ccggtcagcg cctcgtagcc
     4441 catcgcgtag atctggagct ggagcgactc gcgggcccgc tgacgggcct tgaccggatc
     4501 gcggacgtcg ctcgtcttgt agtcggtgat cgtgacctgc tcgcgtccgg tcagctcgag
     4561 tgtcggcgcg acggtgtccg cggacgggtc cggccgatcc ccggcgtcca cgtccgcctg
     4621 cggatccccg gccggttcga tgtcgacccg gtcccaccgg ccccggatcc ggtcgccgcc
     4681 gagggcgaag ctgaactcgc gctcgacgta cgccgggatg acagcacccg ggacgagctg
     4741 ggcggcccgg aagcggcgca gcgtctcgcg cccggcggcg aggcgggcgt cctcgtggtc
     4801 gcgcgacagg aatccctcgt tcgtccaagc ggcctccaac gcggcgatga gctcgtcctc
     4861 ggtcatgacc tcgccgcggg catgacggcg atggaactcc tgcacggccc ggtggagcgc
     4921 cgacccgtag atcatcgcgt gatgcggggg gaccggaacc cggacgacgt gaccgagctt
     4981 gtacttcagc gggcagctga gccaggcatc gatcccggag aacgagaggg tgagcggttc
     5041 gccggtcgga ccgcgcccgg tcgacgggcg gacaggggtc gaggtgaggt tcgcagcctc
     5101 gatccgatcg atggcgttcg cggcccggac gacgggatcg accgcggtcg gcggcaggtc
     5161 cagcgcctcg aggacgaacg gcgacagccg gcggaccccg acgccgccgt agtcgaccgc
     5221 gtgcgacagg atgagctcgt cgcgggcgcg cgtcatcgcg acgtagaaga gtcgccgctc
     5281 ctcacgcagc tggacctcct cgtccggtcc gtcgacgacg tcgccgagca ccgccggcaa
     5341 ggcgagggcg tcgcgccgat tcgggatcgg gaagcggccg gcgacgagac ccggcaggta
     5401 gacgatcggg aactccagcc ccttggcctt gtgcacggtc agcacggcga ccgcgtcgga
     5461 atcgggatcc agatccgcgg tggccgggtc gtcgcccgcc tcgatcaagg tctggagatg
     5521 cccggccacg aacgtcgccc ggtcgtccgc gagcagcgct gactgcgatc ggacgatgtc
     5581 gaagaagcga gcgacgttgc ggagctgctc ctcggccccg accgtcgcct cggcggcgag
     5641 ccggctcagc gtgccggagc gccgcaggaa cgcgtagagg acctcgccgg ccggacgctc
     5701 gtgggcgatg gtcgacagtt cccgcaggtc cgtcacgagc cgggcgaccg ccgtccgcga
     5761 cgcggccgag attcgcagga tcccgggctg gcggtcgatc tcctcgatta cctcccagag
     5821 cgtccggttg cgccgccgtg cgctgttcac gattgcagtc aggtcctcac caccgaggtg
     5881 gtagggctcg ccggtcgcga ccccgtagac gtcgaccgat gatccgagat cggcgatcgc
     5941 ccggaggaag gcgagcagtc gccggacctc cggccgggcg tacagtcccg acgtgccgga
     6001 gaaccgccac ggcaccgcgg cggcatcgag gctgcgcagg atcgggtcgg cgtggccgtt
     6061 ggcgcggacg aggatcgcga tgtcgcgcgg cgcggtcccg gtcgagattc gacccgcgat
     6121 ctcggtcgcg atccggtccg cctcgtcgtg accggacgga tgagtctcca ggcggacggt
     6181 ccgcccggcc ggatcgatcc cccgctccgc gaccaggcgc ttgctgatgc cggcctggac
     6241 ctccagccgg tccggatcgt tgaaccggac gagccggtgg gccgcctcga ggatcgcggg
     6301 tcgcgagcgg tagttgcgcc ggaggacgac ggtccgggcg ttccgatagc ggtcgcggaa
     6361 cccgaggatg ttgctgatcg ccgcgccgcg gaagcggtag atcgactgat cgtcatcgcc
     6421 gaccaccgtc aggttgcggt ggcggtcggc gaccagcgag accagctccg actgggcgcg
     6481 gttcgtgtcc tggaactcgt cgaccaggac atagcggaag cgcgcctgga tctcggctcg
     6541 cgcggccggc gaggtgcgga cgagtcgcag ggccagggcg acctgatcac cgaagtcgat
     6601 gcagccgttc gtctcgagga gctcctggta tcgagcgtag gcccgagcca gctcgcgctg
     6661 gcggccggcc tcctccgcca ggacctcgtc gtccggcgct gctgctgccg cggacgcgag
     6721 cccgtccgca aacgcagcgt atgcctccgg cgagacatcc tcgtccttgc accggctgaa
     6781 cagcgtcgcg agggcagcga ggaaccgggt cgggtcgccg agtggacggt agcgctccag
     6841 ctcgaaggcg aacagatgct cccgcaggaa gatcaccgtc tccgctcggc tcaggacccg
     6901 gatatccgac ggcaatccga gctcgagggc gtactcccgg atgagccggt cgccgaacgc
     6961 atgaaaggtc ccgatcgtcg tgtccgtgta gccgtacgga acgagctggt cgacccggac
     7021 ctgcatctcc gctgccgcct tctcggtgaa ggtgagggcg aggatctccg acggccgcgc
     7081 ccggcgggtc gcgatcagcc aggcgatgcg ccgggtgatg acctgggtct tgccggtccc
     7141 ggcgccagcc acgacgagca gcgggccgtc gccgtgcgtc acggccttca gctgctccgg
     7201 gttgagcccg ccgagcagcg aatccaggcc ggccggggga tcacccgacg gcgtcgtcgg
     7261 accatccgcg agaaagccgt cccagaccgg gatctgctcg aggggaacgg gctcgcggcg
     7321 cgtgcgcgga cggctcatcg tgtcatccag cgcggtcgga tcgggggcca tgacacgatc
     7381 atcgtccggc cccgtgtcac ctagcctgtc aggccggtcc ggcgacccgg tcgcgaacgt
     7441 gctggtcgcg tgccacggcc gcttggccgg cctgggccag gcgcagggcc tcggtctcct
     7501 cctgcgacaa cgcgccgtcc gatcggccgg cgctgacggc cttcgcatag acgcgcgtac
     7561 cagtccggga atggaggctg ctcacgatga agccgttgcc ggccgcgtcc gtcagggcca
     7621 gggcgaagct ctggttgccg ccggtgtcct cgaacgggtt gaaccggacg agcccgaccc
     7681 gctggatcgt ccgccgaccc gtcgcttcca ggaccgccga ccgggccgat agctcgtcga
     7741 gctcgcgggc gaccgcgaag accttgtcga gatgggtgtc gagcacctcg ccgaggctgg
     7801 cgccgtcggc gccgcgggtg agcgaatcca gccggctccg gaggcgaccg atccggcgcg
     7861 tctggacgat cgtgagcagc agcagcccga gcagcacgac ggcgagcccg acgatggcgg
     7921 ctccgacgaa cggcgcgacg aaggcattga ggtcgatcgc ttgggtcctc cgtgcgagcg
     7981 tccgcggcga actcgggcga cgggcggccc gagtctagca ggtgatcctg cacggtcgat
     8041 caccgcgggc cggtcggcga cggacggctg cggtccgccg cgccgggccg aatcgagcgt
     8101 ggcctcgccg tcgggcggcg gccgatgcta tcctccggcc gcaccccacg taccactcgg
     8161 agtttccacc gaccgatggc caagcgctcc cgcctcgcgg cccgacccgg ccagcgacga
     8221 cccctccagc ggccggcagc gcgaccgacc gaaccaaccg accgtccgct cggcagcgtg
     8281 acccgggcgg aagaggcgcg cgcagctgag ctcgaggcag cgatcctcgc cgaggagaag
     8341 gctgcggacg catcgcgcca gacccgggag cgggcacgcc ggccgagcgt cgaatccgtc
     8401 ggcggcgtca gctacacctc cattccgctg tcggtccggg cggccgacga gtacggctac
     8461 gtgaagcgcg acatccggcg gatc
//
LOCUS       DATAIW010000345         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_128807, whole genome shotgun sequence.
ACCESSION   DATAIW010000345 DATAIW010000000
VERSION     DATAIW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128807"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VHM48_10580"
     CDS             complement(<1..1477)
                     /locus_tag="VHM48_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2755901.1"
                     /translation="MNRRLLTAVGATALLLGSALPAATIAASPAPSRQFTRLDVSKID
                     PSIGPAMLANKTVTVMVQLKGKQASTGKATKAQQVAAAKALKASQDKIKPAIVRAGGK
                     VLGQYQYAYNGIKVRIAGRAVAALAAMPGVAAVRNVPTYTIDNVNSVPFTGAPAAWTD
                     LGVTGAGQTIAVIDTGIDYTHANFGGAGTVAAFEANDSTVIEPGSFPTAKVIAGWDFA
                     GDGYDATGDNGSVTPVPDPDPLDCFGHGSHVAGTSAGEGVLADGSTFAGPYNASIYGS
                     NTFAIGPGVAPEASLVDLKVFGCEGSTDLVVDALNWVADYNAHHADAIDVVNMSLGSS
                     FGRNDNPDAVATNNLVDAGVVVVASAGNSGPNAYITGSPAAATQAISVAAEDTLATFP
                     GAVIDFATATDITNANNQNAYPNLPVSGTLKVVFSAPGVVSLGCTAADYGTLPANTIV
                     AIQRGVCAFVDKGAAAQAAGAIGIIDINRDDIANPAELPTFI"
     gene            complement(1722..2648)
                     /gene="mdh"
                     /locus_tag="VHM48_10585"
     CDS             complement(1722..2648)
                     /gene="mdh"
                     /locus_tag="VHM48_10585"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX2755902.1"
                     /translation="MARNKVTVIGAGNVGATTAQRIAEAGLADVVLIDIVEGLPQGKG
                     LDLAEAAPVVGHDSRIVGTNDYADTAGSDIVVVTSGIARKPGMSRDDLMQTNAGIVRA
                     VVEQAAKHSPDAILIIVTNPLDAMCHVAFKASGFPRERVLGMAGVLDSARFRSFIADE
                     LGVSVADTHAFVLGGHGDTMVPLSRYSTVAGVPITELLSPDRVKALEARTANGGAEVV
                     ALLKTGSAFYAPAASSFEMVDAILRDRKRVLPCAVLLQGEFKTHDLFVGVPVVLGRGG
                     MEKVFEIELTEDEQAAFDHSAAAVQELVDKLG"
BASE COUNT          435 a         1003 c         1182 g          503 t
ORIGIN      
        1 cgatgaacgt cggcagctcg gccgggttgg cgatgtcatc gcggttgatg tcgatgatcc
       61 cgatcgcgcc ggccgcctgg gccgccgcgc ccttgtcgac gaacgcgcag acgccgcgct
      121 ggatggcgac gatcgtgttc gcgggcaggg tcccgtagtc ggccgccgtg cagccgaggc
      181 tgacgacgcc cggggcgctg aagacgacct tcagggtgcc gctcacgggc aggttcgggt
      241 aggcgttctg gttgttggcg ttggtgatgt cggtggcggt ggcgaagtcg atcaccgcgc
      301 cggggaacgt cgcgagggtg tcctccgcgg cgacgctgat cgcctgggtc gccgctgccg
      361 gcgagccggt gatgtaggca ttcgggccgg agttgccggc cgaggcgacg acgacgacgc
      421 cggcgtcgac gaggttgttc gtcgcgaccg cgtccgggtt gtcgttgcgg ccgaacgacg
      481 agccgagcga catgttgacg acgtcgatcg cgtcggcgtg gtgggcgttg tagtcggcga
      541 cccagttgag ggcgtcgacg accagatcgg tcgatccctc gcagccgaag accttcaggt
      601 ccacgaggct ggcctcgggc gcgacgcccg gcccgatcgc gaacgtgttg ctgccgtaga
      661 tcgacgcgtt gtagggcccg gcgaaggtcg agccgtcggc gaggacgccc tcaccggcag
      721 aggtgccggc gacgtgcgag ccgtggccga agcagtcgag cgggtcggga tccggcaccg
      781 gcgtgaccga accgttgtca ccggttgcgt cgtagccgtc gccggcgaag tcccagccgg
      841 cgatcacctt ggcggtcggg aaggagccgg gctcgatcac cgtcgagtcg ttggcctcga
      901 aggccgcgac cgttcccgcg ccgccgaagt tggcgtgggt gtagtcgatg ccggtgtcga
      961 tgacggcgat cgtctggccg gcgccggtca cgccgaggtc ggtccacgcg gcgggagcgc
     1021 cggtgaacgg gacgctgttg acgttgtcga tcgtgtaggt agggacattc cggacggcgg
     1081 cgacgccggg catcgcggca agagccgcga ccgcgcggcc ggcgatccgg accttgatcc
     1141 cgttgtaggc gtactggtac tggccgagga ccttgccgcc ggcgcggacg atggccggct
     1201 tgatcttgtc ctggctggcc ttgagggcct tggcggcggc gacctgctgg gccttcgtgg
     1261 ccttgccggt gctcgcctgc ttgcccttga gctggaccat cacggtgacc gtcttgttgg
     1321 ccagcatcgc gggcccgatc gacggatcga tcttgctgac gtcgagccgc gtgaactgcc
     1381 ggctcggggc cggggatgcg gcgatcgtgg ccgcgggcag ggcgctgccg agcagcagcg
     1441 ccgtcgcacc gacggcggtg agcagcctcc ggttcatgtg cctcccttca ggcaatgcct
     1501 gaatctcctc cgtcgcgcgg gcacggcccg aacggcgcca gcggctcccg accgcgggtg
     1561 ggcgacgttg cggacccccg tcacgacact gaacgccgac cctcccgagg tcggcgcttg
     1621 cgtgtgagga ccttacgacc gtcgaccggt cgggtcaatt cggggtggtc gcggaaggtt
     1681 cgatcgaccg gagttggcgg tgcggcgatg caccacggcc gtcagccgag cttgtcgacg
     1741 agctcctgga cggcggccgc agagtgatcg aacgcggcct gctcgtcctc ggtcagctcg
     1801 atctcgaaga ccttctccat cccgccgcgg ccgaggacga ccggcacgcc gacgaacagg
     1861 tcgtgggtct tgaactcgcc ctggaggagg acggcgcacg gcagcacgcg cttccggtcg
     1921 cgcaggatcg cgtcgaccat ctcgaacgat gatgccgcgg gggcgtagaa cgccgagccc
     1981 gtcttgagca gggcgacgac ctccgcgccg ccgttggccg tccgcgcctc gagcgccttg
     2041 acacgatcgg gggacagcag ctccgtgatc gggacgccgg cgaccgtcga gtagcgcgac
     2101 agcgggacca tcgtgtcgcc gtggccgccg aggacgaagg catgggtgtc cgcgacgctg
     2161 acgcccagct cgtcggcgat gaacgaccgg aaccgggcgg agtcgaggac gccggccatc
     2221 ccgaggacgc gctcccgcgg gaagcccgac gccttgaagg cgacatggca catcgcgtcg
     2281 agcgggttgg tgacgatgat caggatcgcg tccggcgagt gcttcgcggc ctgctcgacg
     2341 accgcccgga cgatgccggc gttggtctgc atgaggtcgt cccggctcat gccgggcttg
     2401 cgggcaatgc ccgaagtgac gacgacgatg tcggagccgg cggtgtcggc gtagtcgttc
     2461 gtgccgacga tccgcgagtc gtggccgacg acgggcgcgg cctcggcgag gtcgaggccc
     2521 ttgccctgcg gcaggccttc gacgatgtcg atcaggacga cgtcggcaag gcccgcctcg
     2581 gcgatccgct gggcggtggt cgcaccgaca ttgccggcgc cgatgacggt gaccttgttc
     2641 ctggccatgg cgtgatggtc ctcggctatg gagcggggtt cgggggatgt gcggccattc
     2701 tacggaaggt tgccctccgg aggtccggtg ccggttgcat tggcgaccgc acgatgtcct
     2761 gtccgcggcg acgagccgcg acacgcgacg gatccggttg ttcgtcccga gctgccgatc
     2821 gacggatgac ggaggcgaga actgcggctc ggggcagctg acgtcgcgag cttcctgcca
     2881 gatggtcgat tgacgatcat gtcgcgacgg gaccgctggc cattcctgtc agatggcacc
     2941 cgtggcgtgc aagagccagc gacgatgggt gccggcgacg gtccgggccc ggttctctgg
     3001 cgcagattcg atcggcggcg acgttcggtc tcggcatgtg tcctgccagc gcggcatctg
     3061 gcagggatcc gacggcccca gccgtcccgt ggcccgccac accgacaaat ggcaggaagg
     3121 cgt
//
LOCUS       DATAIW010000346         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_128828, whole genome shotgun sequence.
ACCESSION   DATAIW010000346 DATAIW010000000
VERSION     DATAIW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128828"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VHM48_10590"
     CDS             <1..1418
                     /locus_tag="VHM48_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755903.1"
                     /translation="RRVVLYHGIPVLAVQNITDVGHLRDERFDRGEDRMLVAAGLEHK
                     TAGEIADAYEAAFHADAALLNLLPAHVFPRATEHIPEMIELAQALVDAGHAYVSEEGN
                     VYYAVGSFPDYGALSGNTLDALRAGHRGEVEGDKRDAADFALWKAAGEGRMLKWPTPR
                     WGDGFPGWHLECSAMALRHLGPRFDVHTGGIDNVFPHHEDEIAQSAPIVGGPPARVWV
                     HGEFLNVGGKKMAKSAGNIERIADVAARGIDPLAFRYLCLTSRYRHKLEYTEASLAAA
                     AAGLASLRAGLAALGPPPTEGPWVAPVALRAGAAAARPVGTAEGTVGNGDGAAGVLSD
                     RAHAPGAPLSAAGQSLHDRFSAALDDDLDLPTALAVVRETLRADLPADERRWLVLDAD
                     FVVGLDLDRVVGDRPAADVPIEVADLLTERTAARTERDFAAADALRDRIEQLGYVVID
                     GPEGQTVSPARPGPGASRSGR"
     gene            complement(1372..2316)
                     /locus_tag="VHM48_10595"
     CDS             complement(1372..2316)
                     /locus_tag="VHM48_10595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2755904.1"
                     /translation="MARTRIPDDVLSLAHDRAAARGAQDWAAADRIRAEIEAAGWKIV
                     DRGTDFALEPAHPPTVADGEVVRYGSSEAVPSRLGEPASGIVTVILVATDWPADLDRT
                     LAGLGSHAADGTSIVVVADGPAADQDAGLPADCDGIEVIRTTERLGTAAAWNIGIRRA
                     SGPVIVVLDTSVEPTGDVVGPLVAALDDSTVGVAGGFGIVSDDLRKFDDAPPGDVTAI
                     EGYAIAFRRADAAVRGPLDERFRFYRNLDIWWSLVLRDEGEESAPRRAVAVPIPATRH
                     EHRGWSSLSEAERDRLSKRNFYRIIDRFGWRRDLAAPG"
     gene            2654..4330
                     /locus_tag="VHM48_10600"
     CDS             2654..4330
                     /locus_tag="VHM48_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2755905.1"
                     /translation="MADDVNALAQEYFDYLLMVWPTWGHMMGNYQHADLYDDASRAGE
                     DEEIRRRREFADRAEAIPADDLSAQDRITREMIAFDGRRNADILEARFAEFGADPIFG
                     PVAGLPVYMPKLPIPNADVAEAMIAKTRRIATNFRDMSDRSLEGVARNRTPAAFAVDD
                     TVAQIDRWLARPIGDDPLLNTAEPTGVADADTWRARLRDVIEAEVRPAMAQYRDTLRD
                     QVRSHARDDEHVGLTWLADGDDAYGRMIRYHTTLERSAQEIHEIGLEQVAKLADEYRV
                     LGQEVLGTSDVPEIFRRLRDDPALHHTSGREIVAASKTALAKATAAMGDWFGILPKAG
                     CDVEETQSGAIAFYFPPAKDGSRPGVFFMNTSDPTGWGRYQIEATSYHEGVPGHHLQL
                     AISTELTAVPEFRKRAFIAAYGEGWGLYTERLADEMGLYSTPLDRMGMLEADSMRACR
                     LVVDTGMHALGWSRPKAVDYMTANSPMRVSQIVAEIDRYAVTPGQALAYMIGRLEIQR
                     MRREAEEALGERFAIKGFHDTVLGSGLMPLPVLDRVVREWADGQRAAVLA"
     gene            4362..4838
                     /locus_tag="VHM48_10605"
     CDS             4362..4838
                     /locus_tag="VHM48_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2755906.1"
                     /translation="MNPHDILMYGQRQIDELIDRLRPGDWEAIALGTWTTKDLVGHLG
                     AFESRFVEILTVFLGEEPATNLWQQPLDTFNDDQAAVRREWSVEAILGELRDAHAQSM
                     ALVPRIPAERWREVGTIPWYGPEYALDDLVVYLMYGHKREHSPQLETVLDRTVARP"
     gene            4930..5163
                     /locus_tag="VHM48_10610"
     CDS             4930..5163
                     /locus_tag="VHM48_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755907.1"
                     /translation="MNRTETGRLIALLHAQQREARNRSGEIRGSPSWTESSARLDDLN
                     DQIMHLGVLRSTRDDGDRRAVDPGRRAASDRAG"
     gene            5160..5471
                     /locus_tag="VHM48_10615"
     CDS             5160..5471
                     /locus_tag="VHM48_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755908.1"
                     /translation="MTRGTRSQIHRPPLGGDVFRRPVVDYLAAEQALSRLGAFDPEAA
                     ADVLVGAVLMQGLIDVLSDRPAAEGARRLDAITSTILSGLSPVTAQPDDPSTEPMRRS
                     R"
BASE COUNT          850 a         2012 c         1958 g          764 t
ORIGIN      
        1 tccggcgcgt cgtcctgtac cacggcatcc cggtcctggc ggtccagaac atcaccgacg
       61 tcgggcacct ccgcgacgag cggttcgacc gcggcgagga ccggatgctg gtggcggcgg
      121 gcctcgagca caagaccgcc ggcgagatcg ccgacgcgta cgaggcggcc ttccacgccg
      181 acgccgcgct gctcaacctg ctgccggccc acgtgttccc gcgggcgacg gagcacatcc
      241 ccgagatgat cgagctggcc caggcgctcg tcgatgccgg ccacgcgtat gtcagcgagg
      301 agggcaacgt ctactacgcg gttggctcgt ttcccgacta cggggcgctg tccggcaaca
      361 ccctcgacgc gctgcgcgcc ggacaccgcg gcgaggtcga gggcgacaag cgggacgccg
      421 ccgacttcgc cctgtggaag gctgccggcg agggtcggat gctcaagtgg ccgacgccgc
      481 gctgggggga tggcttcccg gggtggcacc tcgagtgctc ggcgatggcg ctccgacacc
      541 tcggcccacg gttcgacgtc cacaccggcg gcatcgacaa cgtgtttcct caccacgagg
      601 acgagatcgc ccagtcggcc ccgatcgtcg gcgggccgcc ggcccgggtc tgggtccacg
      661 gcgagttcct caatgtcggc gggaagaaga tggccaagtc agccggcaac atcgagcgga
      721 tcgccgacgt ggccgcgcgc ggcatcgacc cgctggcgtt ccgctatctg tgcctcacgt
      781 cgcgctatcg ccacaagctc gagtacaccg aggcctcgct cgccgcggcc gcggccgggc
      841 tggcgtcgct ccgggccggt ctcgcggcgc tggggccgcc gccgaccgaa gggccgtggg
      901 tcgctcctgt cgccctgcgt gccggcgcgg cggctgcccg gcccgtcggc acggccgaag
      961 gaaccgtggg caacggcgac ggtgcggcgg gcgtcctgag cgaccgtgcc cacgccccgg
     1021 gcgcgccgct gtcggccgca ggccagtctc tgcatgaccg gttcagcgcg gccctcgacg
     1081 acgacctcga cctcccgacg gcgctggccg tcgtccggga gacgcttcga gccgacctcc
     1141 ccgccgacga gcggcgctgg ctcgtcctcg acgccgactt cgtggtcggg ctggatctcg
     1201 atcgggtcgt cggggaccgg cccgcggccg acgtcccgat cgaggtcgcg gacctgctga
     1261 ccgagcggac cgctgcccgc accgagcgcg acttcgcggc tgctgatgcg ctccgcgacc
     1321 ggatcgaaca gctcgggtac gtcgtgatcg acgggcccga aggccagacc gtcagcccgg
     1381 cgcggccagg tcccggcgcc agccgaagcg gtcgatgatc cggtagaagt tgcgcttgct
     1441 caggcgatcc cgttcggcct cggacaggga cgaccagccg cggtgctcgt ggcgggtcgc
     1501 cgggatcggg acggccacgg cgcgccgcgg ggcgctttcc tccccttcat cgcgaaggac
     1561 gaggctccac cagatgtcga ggttccggta gaagcggaac cgctcgtcga gggggccgcg
     1621 gacggcggcg tcggcccggc ggaaggcgat cgcgtaaccc tcgatcgcgg taacgtcgcc
     1681 gggtggagcg tcgtcgaact tgcggaggtc gtcgctgacg atcccgaacc cgccggccac
     1741 cccgaccgtc gaatcgtcca gggcggcgac gagagggccg accacgtcgc cggtcggctc
     1801 gacggacgtg tcgaggacga cgatcaccgg tccgctcgcc cggcggatcc cgatgttcca
     1861 ggcggccgcg gtccccagtc gctcggtcgt gcggatgacc tcgatcccgt cacagtcggc
     1921 cggcaggccc gcgtcctggt cggccgccgg cccgtcggca acgacgacga tcgaggtgcc
     1981 gtccgcagcg tgcgagccca ggccggcgag cgtccggtcg agatcggcgg gccagtcggt
     2041 cgcgacgagg atgaccgtca cgatgccgct cgcgggttcg ccaaggcgcg atgggaccgc
     2101 ctcagacgat ccgtagcgca cgacctcgcc gtcggcgacg gtcggtggat gcgccggctc
     2161 gagggcgaag tccgtgccac ggtcgacgat cttccagccg gcggcctcga tctcggcccg
     2221 gatccgatcg gccgccgccc agtcctgcgc gccgcgcgcc gcggctcggt cgtgggcaag
     2281 ggagagaacg tcatcgggaa tccgggtgcg ggccacggcg gaaggatagc ccgcaggtcg
     2341 gatgcgcctc gctatagacg cgcctcgcta ccgtccggcg actgaaccgc accgggcgcg
     2401 tgctcagccc gcccggtagg gtgccgtctc gtcgatgttg gcgacgaact ccggcaaccc
     2461 gacatgcccg agcacctggc gccacgcgcg agctcggatc gagtgaagcg gagctgctcg
     2521 acgagcgggt ggatcgacgg ggttgccatg gtcggaatct ggcccgccac gggccgcccc
     2581 gcaacccagc cgacgcgcgt acgatcgagg tcacacaacc agagcccgga tgcgcgcacg
     2641 gagacatcga ccaatggccg acgacgtcaa cgccctcgcc caggagtact tcgactacct
     2701 cctgatggtg tggccgacct ggggtcacat gatgggcaac taccagcacg ccgacctcta
     2761 cgacgatgcc agccgagcgg gcgaggacga ggagatccgc cgccgtcgcg agttcgccga
     2821 ccgggccgag gcgatcccgg ccgacgacct gtccgcccag gaccggatca cccgcgagat
     2881 gatcgcgttc gacggacgcc gcaacgccga catcctcgaa gcccgcttcg ccgagttcgg
     2941 ggccgatccc atcttcgggc cggtcgccgg cctgcccgtc tacatgccga agctgccgat
     3001 cccgaacgcc gacgtggccg aggcgatgat cgccaagacc cgccggatcg cgacgaactt
     3061 ccgcgacatg agcgaccgat cgctggaggg ggtcgcccgg aaccggacgc ccgccgcctt
     3121 cgccgtcgac gacaccgtcg cccagatcga ccgctggctc gcccgtccga tcggcgacga
     3181 ccccctcctg aacacggccg agccgacggg cgtggccgat gccgacacct ggcgggcgcg
     3241 actccgcgac gtgatcgagg ccgaggttcg cccggccatg gcgcagtacc gggacacgct
     3301 ccgcgaccag gtccggtcgc acgcccggga cgacgagcac gtcgggttga cctggctggc
     3361 ggacggcgac gacgcctacg gtcggatgat ccgctaccac acgacgctcg agcgctcggc
     3421 ccaggagatc cacgagatcg gcctggaaca ggtcgcgaag ctcgccgacg agtaccgcgt
     3481 cctcggccag gaggtgctcg gaacgagcga cgtgcccgag atcttccgtc gtctgcgcga
     3541 cgacccggca ctccaccata cgagcggccg ggagatcgtc gccgcgtcga agaccgcgct
     3601 ggcaaaggcg accgcggcga tgggcgactg gttcgggatc ctgccgaagg ccggttgcga
     3661 tgtcgaagag acccaatccg gcgcgatcgc gttctacttc cccccggcga aggacggcag
     3721 ccgacccggc gtgttcttca tgaacacgtc cgatccgact ggctggggtc gctaccagat
     3781 cgaggcgacc tcctatcacg agggcgtccc cggccatcac cttcaactcg cgatctcgac
     3841 cgagctgacc gccgtcccgg aattccgcaa gcgtgccttc atcgccgcct atggcgaggg
     3901 ctggggcctc tacacggagc ggctcgccga tgagatgggc ctctattcca ctccgctcga
     3961 ccggatgggc atgctcgagg ccgattcgat gcgggcatgc cggctcgtcg tcgataccgg
     4021 gatgcacgcc ctcggctgga gccgcccgaa ggcggtcgac tacatgaccg cgaactctcc
     4081 gatgcgggtc agccagatcg tggcggagat cgatcgctac gcggtgacgc cgggccaggc
     4141 gctcgcctac atgatcggcc ggctcgagat ccagcggatg cggcgcgagg ccgaggaggc
     4201 cctcggcgaa cggttcgcga tcaagggctt ccacgacacg gtgctcggct ccgggctcat
     4261 gccgctgccg gtcctcgacc gggtcgttcg cgaatgggcg gacggccagc gagccgcggt
     4321 cctggcgtga tcgatccgaa cgcgttcgtg ggcgcgtcac gatgaacccc cacgacatcc
     4381 tgatgtatgg ccagcgccag atcgacgagc tcatcgatcg gctccggccc ggcgactggg
     4441 aggcgatcgc gctcgggacc tggaccacga aggacctcgt cgggcacctc ggcgcgttcg
     4501 agtcccgctt cgtcgagatc ctgacggtgt tcctcggtga agagcccgcg acgaacctct
     4561 ggcagcagcc actcgacacg ttcaacgacg accaggccgc cgtccgtcgc gagtggtcgg
     4621 tcgaggcgat cctcggggag cttcgcgacg cccacgccca gtcgatggcg ctggtcccgc
     4681 ggatcccggc cgagcgctgg cgtgaggtcg gcacgatccc gtggtacggg ccggagtacg
     4741 ccctcgacga tctcgtggtc tacctgatgt acggccacaa gcgcgaacac agtccccaac
     4801 tcgagaccgt cctcgaccga actgtcgcgc ggccgtagcg tccgccgccg ctggtggtct
     4861 gaccctgtcg gcagcgcggc cgtagcgttc cgcacgggtc gatcggaccc cgggaaggac
     4921 ggcgacatcg tgaatcggac ggagaccgga cggctgatcg ccctcctgca cgctcaacag
     4981 cgcgaggccc gcaatcgatc cggcgagatt cgtggtagcc cgagctggac cgaatcgtcg
     5041 gcccgactcg acgacctgaa cgaccagatc atgcacctcg gggtgctgcg atcgacccgc
     5101 gacgacggcg atcgccgcgc agtggatccc ggccgacggg cggcgagcga ccgggcgggt
     5161 tgacacgggg aacccgctcg cagatccatc gaccgcccct cggcggtgac gtgttccgcc
     5221 ggccggtggt cgactacctc gccgccgaac aggccctcag ccggctcgga gcgttcgatc
     5281 ccgaggcggc ggccgacgtg ctggtcggcg cggtcctgat gcagggcctg atcgatgtcc
     5341 tcagcgatcg accggcggcc gagggcgccc gccggctcga cgcgatcacc tccacgatcc
     5401 tgtccgggct ctcgcccgtc acggcgcaac cggacgaccc ctcaaccgaa cccatgcgga
     5461 ggagtcgatg acagctgcac ccctgcccga gccgaccacc gagacgaccg gtcgggtgat
     5521 ccggacgccg tggcgatacg acctcggcct gtggctcggg tcgtggggcg gcgagggccg
     5581 gttc
//
LOCUS       DATAIW010000347         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_188535, whole genome shotgun sequence.
ACCESSION   DATAIW010000347 DATAIW010000000
VERSION     DATAIW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188535"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VHM48_10620"
     CDS             complement(<1..1344)
                     /locus_tag="VHM48_10620"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX2755909.1"
                     /translation="MTQSTTRPKTPSAAGAAPAIGRIVRANLPTAELYEDAVRAGEGL
                     IAADGPLVVRTGKHTGRSPKDKFIVREPGSAAKIWWGDVNRPIALEHYDRLRARLMAY
                     LADRPVYAQDCFIGAHPAHRRSLRVYTETAWASIFARNLFRRPSAAELADFAPNFTII
                     DVPSFQADPTTEGVRTGTAILVNLAAMEIIIVGTEYAGEIKKSAFTVMNYLMPDEGVL
                     PMHSAANVGKDGESVVFFGLSGTGKTTLSADPERSLIGDDEHGWGVDGVFNFEGGCYA
                     KTIRLSPMYEPDIFQTTRRFGTILENVDLKPATRELDLDSERFTENTRGAYPLHFIGN
                     ADPTGMAGQPTNVVLLTADAFGVLPPISRLTHDQAAYHFISGYTAKLAGTEIGVKEPQ
                     ATFSACFGAPFMPRHPGEYAAMLAERLERYGVRAWLVNTGWTGGPYGTGERMNINH"
     gene            complement(1610..>1811)
                     /locus_tag="VHM48_10625"
     CDS             complement(1610..>1811)
                     /locus_tag="VHM48_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2755910.1"
                     /translation="AEATRLFTLAESLGGVESLVEIPATMTHASVAGSKLEVDPALVR
                     LSVGIEHVDDLIRDLGNALDHA"
BASE COUNT          248 a          570 c          698 g          295 t
ORIGIN      
        1 gtggttgatg ttcatccgct cgcccgtgcc gtacggtccg ccggtccagc cggtgttcac
       61 gagccaggca cggacgccat agcgctcgag gcgctcggcg agcatcgcgg cgtactcgcc
      121 cgggtggcgc ggcatgaacg gggcgccgaa gcaggccgag aaggtcgcct gcggctcctt
      181 cacgccgatc tcggttccgg cgagcttggc ggtgtagccg ctgatgaagt ggtaggccgc
      241 ctggtcgtgg gtgaggcgcg agatcggcgg caggacgccg aacgcatcgg ccgtgaggag
      301 cacgacgttc gtcggctggc cggccatccc ggtcggatcg gcattgccga tgaagtggag
      361 cgggtaggcg ccgcgtgtgt tctcggtgaa gcgctcggag tcgaggtcca gttcgcgggt
      421 cgccggcttc aggtcgacgt tctcgaggat cgtcccgaac cggcgggtcg tctggaagat
      481 gtccggctca tacatcgggc tgaggcggat cgtcttggcg tagcagccgc cctcgaagtt
      541 gaagacgccg tcgacgcccc aaccgtgctc gtcgtcgccg atcaagctcc gctccggatc
      601 ggcggagagg gtcgtcttgc ccgtcccgct gagcccgaag aagacgaccg actcgccgtc
      661 cttgccgacg ttcgccgccg agtgcatcgg caggacgccc tcgtcgggca tcaggtagtt
      721 catgacggtg aacgccgact tcttgatctc gccggcgtac tcggtgccga cgatgatgat
      781 ctccatcgcg gcgaggttga cgaggatcgc ggtcccggtc cggacgccct cggtcgtcgg
      841 gtcggcctgg aacgacggga cgtcgatgat cgtgaaattc ggagcgaagt cggcgagctc
      901 tgcggcggac ggccgacgga acaggttgcg ggcgaagatg ctggcccagg cggtctcggt
      961 gtagacgcgg aggctgcgcc gatgggccgg gtgggcgccg atgaagcagt cctgggcgta
     1021 gaccggccgg tcggccaggt aggccatgag ccgggcgcgg agccgatcgt agtgttcgag
     1081 ggcgatcggg cgattgacgt cgccccacca gatcttcgcg gcggagcccg gctcccggac
     1141 gatgaacttg tccttcggcg agcggccggt gtgcttgccc gtccggacga cgagcggccc
     1201 gtctgcggcg atcaggccct cgccggcccg gaccgcgtcc tcgtacagct cggcggtcgg
     1261 caggttggcc cggacgatcc ggccgatggc cggggcagcg ccggccgcgg atggggtctt
     1321 cgggcgggtc gtggactgcg tcatgggatc ggactccttg cgcgcgggcg atcactcccg
     1381 atcgaccggc cgcgcgtggc tcgcgggctc cgggcgccgc ggcggcgccc gaggtgagcg
     1441 aggaagtcgg cttccgcagt cgctgttcgc ggcatcgtat cgcaaccggg gcgcaacctg
     1501 tccgcaaccc gaacgtcctg ggacgtgtca gctgtgtgtc aggttcacgg ggccggcgcg
     1561 gacgcctttc cgttcggtga cgaggcgtcg ccgatgccgg gctgggcggt caggcgtggt
     1621 cgagggcgtt gccgaggtcg cggatcaggt cgtcgacgtg ctcgatgccg accgagaggc
     1681 gaaccagggc ggggtcgacc tcgagcttcg agccggcgac cgacgcgtgg gtcatcgtcg
     1741 ccgggatctc gacgagcgac tccacccctc cgagggattc ggcgagggtg aagagccggg
     1801 tggcctcggc g
//
LOCUS       DATAIW010000348         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_218309, whole genome shotgun sequence.
ACCESSION   DATAIW010000348 DATAIW010000000
VERSION     DATAIW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218309"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VHM48_10630"
     CDS             complement(<1..862)
                     /locus_tag="VHM48_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755911.1"
                     /translation="MSIEPPQRPRRPRTSRALTGDAIAPLYDPAFEHDACGIGFVADA
                     GGTRLGEVLPLALGGLAALAHRGAFGADGASSDGAGVSIPLDPTVVEALVPGSGLAAG
                     RPAILQLFLPRTGAGARNGAARRLVAERLAADGLEVVHWRTVPTDPSALGGAARACRP
                     AFVQAFVRRPSDGRGSPLTDAAFERRLVLVRRRIETAAQAAGLGDAVAIPSASCRTLV
                     YKGLVAGARLADLYPDLRAPGIRVRYALFHQRYATNTQPTWRLAQPFRSIAHNGEINT
                     VRGNRSQVRVE"
     gene            complement(1009..>2212)
                     /locus_tag="VHM48_10635"
     CDS             complement(1009..>2212)
                     /locus_tag="VHM48_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2755912.1"
                     /translation="LMSTALVMIMLPGLALFYGGLVRRKNVLSTIMHSFFGLAIVSVV
                     WVLIGFSLAFGPDANLFGIPGLVGNLDFVGFMNVGLEPSTVYATSIPFVLFAAFQLMF
                     AAITPALITGAFAERKRFASFVLFTILWSIVVYSPIAHWVWASDGWLFKLGALDFAGG
                     TVVHASSGLSALIVALLIGKRVASAEKIEPHDIPMTVLGAGLLWFGWFGFNAGSALTA
                     GGLAASAFIVTNTAAAAATLTWVGASYLMHRKVSVVGAACGAVAGLVAITPASGFVTP
                     GGAILIGLAAGGLCYSATLLRERIKIDDALDVFAVHGVGGVFGALATGVLATSAVQEK
                     YFGLIDGNAGQVVTQLIAVGAVIAYAVVATFVIIKLVDAILGIRISAKDEEMGIDLST
                     HGEVAYSA"
BASE COUNT          378 a          725 c          846 g          263 t
ORIGIN      
        1 cctctacccg gacctgcgag cggttgccac ggaccgtgtt gatctcgccg ttgtgggcga
       61 tcgagcggaa cggctgggcc agccgccagg tcggctgcgt gttggtcgca tagcgctggt
      121 ggaacagggc gtaacggacc cggatcccgg gcgctcgcag gtccgggtag aggtccgcca
      181 ggcgggcacc ggcgacgagg cccttgtaga cgagcgtccg gcatgaggcc gaggggatgg
      241 cgacggcgtc accgagcccg gcggcctgcg cggcggtctc gatccggcgg cggacgagga
      301 cgagccgtcg ctcgaaggcc gcgtcggtca gcggacttcc gcgcccatcc gacgggcgac
      361 ggacaaacgc ctggacgaag gcagggcggc aggcgcgtgc ggcgccgccg agcgccgacg
      421 gatccgtcgg cacggtccgc cagtgaacga cttcgaggcc gtcggcggcg agacgctccg
      481 ccacgagtcg acgggccgcg ccgttccggg cgccggcacc ggtccgcggc aggaagagct
      541 gcaggatcgc cggccgcccg gccgcgaggc cgctgccggg gacgagtgcc tcaacgacgg
      601 tcggatcgag cgggatcgac acgcccgcgc cgtcggacga cgcgccgtcc gccccgaacg
      661 cgccgcgatg ggccagggca gcgagaccgc cgagggccag cggcaggacc tccccgaggc
      721 gcgtgccgcc ggcgtcggcg acgaacccga tgccgcaggc gtcgtgctcg aaggcggggt
      781 cgtagagcgg ggcgatcgcg tcgccggtga gggcgcggga ggtccgcgga cggcgaggtc
      841 gctgtggtgg ctcgatggac atcgatggcg ctccgtcggt ggagtcggag tggagtcgga
      901 gtggagtcga accgaccgcc cgggaggagc cgggcggtcg gtcgttgtcg ttcgaagggc
      961 gatggtcggg ccggcggtcg cctcgggagg gcgcccgccg cgccgggcct acgccgagta
     1021 ggcgacctcc ccgtgggtcg agagatcgat ccccatctcc tcgtccttgg ccgagatccg
     1081 gatgccgagg atcgcgtcga cgagcttgat gatcacgaac gtcgcgacga cggcgtaggc
     1141 gatgaccgcg ccgacggcga tcagctgggt cacgacctgc cctgcgttgc cgtcgatcag
     1201 gccgaagtac ttctcctgga cggcgctcgt cgcaaggaca cccgtcgcga gggccccgaa
     1261 gacgccaccg acaccgtgga cggcgaagac gtcgagcgcg tcgtcgatct tgatccgctc
     1321 gcggaggagg gtcgcgctgt agcagaggcc gccggcggcg aggccgatca ggatcgcgcc
     1381 gcccggagtc acgaagcccg acgcgggcgt gatcgcgaca agcccggcga cggcgccgca
     1441 ggcggcaccc acgacgctga ccttgcggtg catgaggtag ctcgcgccga cccaggtgag
     1501 agtcgcggcc gcggcggcgg tgttcgtgac gatgaacgcg ctggccgcga gcccacccgc
     1561 agtgagtgcc gagccggcgt tgaagccgaa ccagccgaac cacaggaggc cggcgccgag
     1621 gacagtcatc ggaatgtcat gcggctcgat cttctcggcg ctcgcgaccc gcttgccgat
     1681 caggagggcg acgatcaggg ctgacaggcc ggagctggcg tggacgaccg tgccgcccgc
     1741 gaagtcgagc gcgccgagct tgaacagcca gccgtcggac gcccagaccc agtgggcgat
     1801 cggggagtag acgacgatcg accagaggat cgtgaacagg acgaacgagg cgaaccgctt
     1861 gcgctcggcg aacgcgcccg tgatcagggc cggcgtgatt gcggcgaaca tcagctggaa
     1921 ggcggcgaac agcacgaagg ggatcgacgt cgcgtagacc gtgctgggct cgagcccgac
     1981 gttcatgaac ccgacgaagt ccaggttgcc gaccaaaccc ggaatgccga acaagttggc
     2041 atcaggcccg aacgccagcg agaacccgat gaggacccag acgacgctga cgatcgcaag
     2101 cccgaagaag ctgtgcatga tcgtcgagag cacgttcttg cggcgaacga ggccgccgta
     2161 gaagagggcc agccccggca acatgatcat cacgagcgcc gtggacatca gc
//
LOCUS       DATAIW010000349         6343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_112084, whole genome shotgun sequence.
ACCESSION   DATAIW010000349 DATAIW010000000
VERSION     DATAIW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112084"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VHM48_10640"
     CDS             complement(<1..767)
                     /locus_tag="VHM48_10640"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX2755913.1"
                     /translation="MRVRRGLLFWGLFLIPLGGISLLVRAGAVDPDRLSEAWRLWPLI
                     LIGIGLAILLGPGRTAVVGTAIVAVVLGSVGGAAIASGHLWIGALSDCAVASGPTDQQ
                     LQRSGSFDRSAAVRLDLRCGSLALTTTTGSQWKLDAAYRGTGPIVDASGSRLDLRVSG
                     GGGERRNDWTVSLPSAVGVLSITANGATATLQFPDGHVASLDVDMNAGDLRIDATHGA
                     VDVVDLSMNAGRIRLDAGSSVLGGDVSVNAGAFDLCVP"
     gene            complement(1023..3080)
                     /locus_tag="VHM48_10645"
     CDS             complement(1023..3080)
                     /locus_tag="VHM48_10645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2755914.1"
                     /translation="MTTALRPDGGFDADPSGALRPFDPMPVSILRATVSRRALAVSWT
                     LVAQIGVAGLAAALYLVNLTVSGFANTYYSAAALSASQSWSAWFFGSIDASNFITVDK
                     PPLATMVMGLSVRLFGLSSWSVLLPEALMGVGTVLVLMAIVRRTMGSPAAIIAGLVAA
                     LTPAAVLIFRYNNPDALLTLLLVGSAYAFVRSLERGSLRWVALAAILVGLAFNTKYLQ
                     GWLVLPAFAAVWAIAAPGGVRHRILGLAVALAATIVASGWWILVMELLPVSARPFVGG
                     STNGTAIDLVLGYDGLGRIFGGSGGPGGGGAGAGGFSGTPGLFRLFNAQLGGQGAWFL
                     PLAGLGLGVGLWARRHAPRTDLRRAAYLMWGLWLAIHVVVFSFMSGVIHSYYVVVMAP
                     AIGALVGGGVVELWAMRARAPHRPWAGILLAGGLAATAAIAWLLLGRTPDFVPGLAVG
                     VVAVTGAAALVIALPARVVARPIQLAAIALGFAVLLAGPATYALDTMQTAYSSGDPAA
                     GPQAVGQGGNGAGFGGPGGPGGQLPSGFTPGGAPGGATGGANGGLPGGTGAGGAFGGS
                     GDQAVNSSLTAYLVANRGTASWIVAVTSANQAGSIELATGEPVMAMGGFTGSDPAPTL
                     EQFQAFVASGQLRFVIVGGQDGGPGGGTDSTARTAWIQANCRAVTSVSSSLYDCAGLT
                     TGG"
     gene            complement(3146..3688)
                     /locus_tag="VHM48_10650"
     CDS             complement(3146..3688)
                     /locus_tag="VHM48_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX2755915.1"
                     /translation="MTLLARDRSGTVSRTHRPTVVEHVDSPPSTSDGRATNPSGTVEP
                     RGLRSQLLRFGAIGVASTLAWAGLYTLLRGAGLGSVAANGLALVVTTVANTAANRRLT
                     FGVTGREGLARDHGAGLLAFGLAIGLTTATATILDRAAPGANRLVELAVLSLANVAAT
                     AGRFVLLRAWVGRASPLRLT"
     gene            complement(3690..4037)
                     /locus_tag="VHM48_10655"
     CDS             complement(3690..4037)
                     /locus_tag="VHM48_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755916.1"
                     /translation="MKHDPRPHIHRRRRAIGRLRSMTTGAAIAGFAGTVGFGVLAAAT
                     WSGNATAADAAGGSTTTLNGTNDTNGTTGTSGTSRTRIVPNTGGTAQQPTTTTPRVQR
                     GSGTGHASTGGSH"
     gene            complement(4132..5358)
                     /locus_tag="VHM48_10660"
     CDS             complement(4132..5358)
                     /locus_tag="VHM48_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2755917.1"
                     /translation="MSAVSALPSVRRTSIRVALAATAVVAVAYFAVAVAVVVIVQRNL
                     TDQVDQRITSSFGRVPHDGDGGGGPYEPPPPERFGGAPFVRWSVESDGSILTEPSNPA
                     LPVEYASVAAPVTATIGGYEFRIAGRQNGDDWLVIGQSLDAVTETRGTVVLAELVIAP
                     LLLGIVFLGAVAIGRRVAAPIEVSRQRQLDFTADASHELRTPLAVIEAHTSLALAQDR
                     DVGWYRSAFSRIDHESKRMRRMLEDLLWLARFDASKRPPNIEPVDLAVMAAQTVDRFG
                     AIAETRHLDIDLDAPADGAVIAAPPEWLDRLLGVLLDNACKYAPEGGRVDITVTVDGS
                     RVALTVDDSGPGIPEAERTRIFDRFHRATDAQGGAGLGLAIADAIVRATGGRWKVASA
                     PGGGARMGISWSRALA"
     gene            complement(5355..6023)
                     /locus_tag="VHM48_10665"
     CDS             complement(5355..6023)
                     /locus_tag="VHM48_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755918.1"
                     /translation="MRILVAEDDPGLRDVIVLGLADSGYHVDAVDRGDDAIDQLKFYD
                     YDVAILDWRMPGAEGIEVVEWARRNRRPTAILLLTARDTPADRIRGLDSGADDYLVKP
                     FDFGELLARVRALQRRPRGVDGPVIERGRLVLDPVTRVVTVDGRELGLTSTEYRILEL
                     LMRRAPAVVDRKAIAEHGWADETEPLGSNAIDVQLSRIRAKLPNAGIRIVTVRGAGYR
                     LEDA"
     gene            complement(6028..>6343)
                     /locus_tag="VHM48_10670"
     CDS             complement(6028..>6343)
                     /locus_tag="VHM48_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2755919.1"
                     /translation="FRSARTARVGTSEPATMRTDLVVDRIACDGFGMCAELLPELIEL
                     DDWGYPIVAAGGVPDALLDHAKRAVAVCPVLALRLARVPIAPPERGAAGWLGNRSPSV
                     RR"
BASE COUNT          973 a         2222 c         2367 g          781 t
ORIGIN      
        1 ggaacacaca gatcgaaggc gcccgcgttc accgagacgt caccgccgag aacgctcgaa
       61 ccggcgtcca gccggatccg cccggcgttc atcgacaggt cgacgacgtc gactgcccca
      121 tgcgtcgcgt cgatccgcag gtcgccggcg ttcatgtcca cgtcgaggct ggcgacgtga
      181 ccgtcgggaa actgaagcgt cgcggtcgcg ccattggcgg tgatcgacag caccccgacc
      241 gcgctcggca ggctgaccgt ccagtcgttc cgccgttcgc caccgccacc cgacacgcgc
      301 agatcgagcc ggctccctga ggcgtccacg atcggcccag tgccccgata ggccgcgtcg
      361 agcttccact gactgcccgt cgtggtcgtg agggcaagcg aaccgcagcg gagatcgagg
      421 cggaccgctg ccgacctgtc gaaggagccc gaccgctgga gctgctggtc cgtcggaccg
      481 ctggcgacgg cgcaatccga cagcgcgccg atccacaggt gcccgctggc gatggccgcc
      541 ccgccgaccg agcccaggac cacggccacg atcgccgtcc cgacgaccgc tgttcggccc
      601 gggccgagca ggatcgccag cccgatcccg atcaggatga gcggccacag ccgccacgct
      661 tcggacaggc gatcggggtc gacggcaccg gcccggacca ggagggagat cccgccgagc
      721 gggatcagga acaagcccca gaagagcagg ccgcgtcgaa cgcgcatggg cgcccaccat
      781 ccaattcttg tgctggatgc gtgcgtcgcc tcccgcgacg acgccgccgc cacgtcgggc
      841 ggccgcgtca tcctaggaag tcgcggcctc gacgtctgct gccgcatgac cttggccggt
      901 cgggccgttc gtcacgatcg gtgcgcagcc ggagggcggc tcatcggcgg gcggaatacg
      961 cgatcggccg ccatcgcact catggcgcga tggcggccgt gaccgggccg gactgggggg
     1021 cgtcagccgc cggtggtcag gccggcacag tcgtagaggc tcgagctgac gctcgtgacc
     1081 gcgcggcaat tggcctggat ccacgccgtc cgtgccgtcg aatcggtgcc tccgccgggt
     1141 cccccgtcct gcccgccgac gatcacgaag cggagctggc cgctcgcgac gaacgcctgg
     1201 aactgctcga gggtcggggc cgggtcgctg ccggtgaagc cgcccatcgc catgacgggc
     1261 tcaccggtcg cgagctcgat cgagccggcc tggttggccg aggtcacggc cacgatccag
     1321 ctggcggtgc cgcggttggc gacaaggtag gcggtcagac tggagttcac ggcctgatcg
     1381 ccggaccctc cgaacgcccc gccagcgccg gttccgccgg ggagtccacc gttcgcgccg
     1441 ccggtggcgc cacccggggc gccgcccggg gtgaagccgc tcggcagctg gccgcccgga
     1501 ccgcccggac cgccgaagcc agcgccgttg ccgccctgcc cgacggcctg gggtcccgcg
     1561 gcggggtcgc cgctgctgta cgcggtctgc atcgtgtcga gggcataggt cgccggtcca
     1621 gcgaggagga cggcgaaccc gagcgcgatc gccgcgagct ggatcggtcg agccacgacg
     1681 cgcgccggga gggcgatcac gagggccgcc gccccggtca ccgcgacgac gccgaccgcc
     1741 aggcccggca cgaagtccgg tgtccgcccg agcaggagcc aggcgatcgc cgccgtcgcc
     1801 gccaacccgc cggcgaggag gatcccggcc cacggtcgat ggggcgcgcg agcgcgcatc
     1861 gcccacagct cgacgacgcc gccgccgacg agggcgccga tggcgggcgc catcacgacg
     1921 acgtagtagg aatggatcac gcccgacatg aagctgaaga cgaccacgtg gatcgccagc
     1981 cacaggcccc acatcaggta ggcggcgcgg cgaaggtccg ttcgcggcgc gtgccgccgc
     2041 gcccagagcc cgacgccgag gccgagcccg gcgagcggca ggaaccacgc gccctggccg
     2101 ccgagctggg cgttgaagag gcggaacagg cccggcgtgc cgctgaagcc gccagcgccg
     2161 gctccaccac cgccgggacc gccgctgccg ccgaagatcc ggccgaggcc gtcatagccg
     2221 aggacgaggt cgatcgcggt cccgttggtc gagccgccga cgaacggccg ggccgacacg
     2281 ggcaacagct ccatcacgag gatccaccag ccgctcgcga cgatcgttgc cgccagggcg
     2341 accgccaggc ccaggatccg gtggcggacg ccgccgggtg cggcgatcgc ccagacggcg
     2401 gcgaacgccg gcaggacgag ccagccctgc aggtatttcg tgttgaaggc caggccgacc
     2461 aggatcgcgg ccagggccac ccagcgcagg ctgccccgct cgagcgagcg aacgaacgcg
     2521 tacgccgagc cgacgagcag gagggtcagg agggcgtccg ggttgttgta gcggaagatc
     2581 aggaccgcgg ccggcgtcag cgcggcgacg agcccggcga tgatcgccgc gggcgagccc
     2641 atcgtccgcc ggacgatcgc catcaggacg aggaccgttc cgacgcccat cagcgcctcc
     2701 ggcaggagga cgctccacga gctcaggccg aacagccgga ccgacaggcc catgaccatc
     2761 gtcgcgagcg gcggcttgtc gaccgtgatg aagttgctcg cgtcgatcga accgaagaac
     2821 caggccgacc agctctgcga tgcggacagc gcggccgcgg agtagtaggt gttggcgaag
     2881 ccgctcacgg tcaggttgac gagatacagg gccgccgcga gtccggcaac accgatctgg
     2941 gcgacgagcg tccacgacac ggcgagcgcg cgccgcgaca cggtggcgcg caggattgag
     3001 acgggcatgg gatcgaaggg ccggagggct cccgacgggt cggcatcgaa gccgccgtcc
     3061 gggcgcaggg cagtcgtcat cagggacgtc cttttcgtaa cgggatgcga gtggcggaag
     3121 gaggggtcgc ccgaggcgac cgacgtcagg tgaggcgcag gggcgacgcg cgcccgaccc
     3181 acgcccggag gagaacgaag cggccggcgg tcgcggccac gttggccagg ctgaggacgg
     3241 ccagctcgac gagccggttc gcgccaggcg cggcccgatc gaggatcgtc gcggtcgccg
     3301 tggtgagccc gatcgcgagt ccgaatgcga ggaggccagc gccgtggtcg cgggcgagtc
     3361 cctcgcgacc ggtcacgccg aaggtcagcc gtcgattggc cgcggtgttg gcgacggtcg
     3421 tcacgacgag ggcgagtcca ttcgcggcga cgctgcccag gccggctcca cggaggaggg
     3481 tgtagaggcc ggcccaggcg agcgtgctcg cgacaccgat ggcgccgaac cggaggagct
     3541 gcgatcggag accgcggggc tccaccgtcc cggatggatt ggtcgcccga ccgtcggagg
     3601 tcgacggggg ggagtcgacg tgctcgacga cggtcggacg atgggtgcgc gagacggtcc
     3661 cggaccgatc acgcgcaagg agcgtcatgt cagtgcgatc cacccgtcga cgcgtgaccc
     3721 gtgccgcttc cacgctggac ccgcggggtc gtggtcgtcg gctgctgggc ggtcccgccg
     3781 gtgttcggga cgatccgggt gcggctcgtg cccgacgtgc cggtggtccc gttcgtgtcg
     3841 ttcgtgccgt tcagtgtcgt cgtggatcca ccggcggcat cggccgcggt cgcgttgccc
     3901 gaccaggtcg cggcggcgag gacgccgaag cccaccgtac cggcgaaacc ggcgatggcg
     3961 gcaccggtcg tcatcgatcg caggcggccg atcgcgcgac gacggcggtg gatgtgcgga
     4021 cggggatcgt gcttcatgac cgcagtttca tcgatgctcc ctttcggaag gattacgcga
     4081 tcgctccgaa cagcggaaga tttcgtcacc cgcggacgcc tgcgggccgc ctcaggcgag
     4141 ggcacgactc cagctgatcc ccatccgggc gcccccgccc ggcgcgctcg cgaccttcca
     4201 gcggccgccc gtcgcccgga cgatcgcatc ggcgatcgcg agcccgaggc ccgccccgcc
     4261 ctgggcatcg gtcgcgcggt ggaaccgatc gaagatccgg gtccgctcgg cttccgggat
     4321 gccgggcccg ctgtcgtcga ccgtcagcgc cacccgcgag ccgtcgacgg tcaccgtgat
     4381 gtcgacgcga ccgccttcgg gcgcgtactt gcaggcgttg tcgaggagga caccgagcag
     4441 gcggtccagc cactccggcg gggcggcgat gaccgcgccg tccgctggag catcgagatc
     4501 gatgtcgaga tggcgcgtct cggcaatcgc cccgaaccgg tccacggtct gggccgccat
     4561 gaccgcgagg tcgacgggct cgatgttggg aggtcgcttc gatgcgtcga agcgggcaag
     4621 ccagaggaga tcctcgagca tccgccgcat ccgcttcgac tcgtggtcga tccggctgaa
     4681 cgccgagcgg taccagccga cgtcgcgatc ctgggcgagg gcgaggctcg tgtgggcctc
     4741 gatgacggcg agcggggttc gcaattcgtg ggaggcgtcg gcggtgaagt cgagctggcg
     4801 ctgacgggag acctcgatcg gggcggccac tcgccgcccg atcgcgaccg cgccgaggaa
     4861 gacgatcccg agcaggagcg gcgcgatgac gagctcggca aggacgaccg tgccgcgcgt
     4921 ctccgtcacg gcgtccagcg actggccgat gacgagccag tcgtcgccgt tctggcggcc
     4981 cgcgatccgg aactcgtagc cgccgatcgt ggccgtgacg ggcgccgcga cgctcgcgta
     5041 ctcgacgggc aaggccggat tcgacggctc cgtcaggatc gagccgtccg attcgaccga
     5101 ccaccgcacg aacggcgcgc cgccgaatcg ttcgggcggc ggcggctcgt acggaccgcc
     5161 gcccccgtcg ccgtcgtgcg gaacgcgacc gaacgacgac gtgatccgct ggtcgacctg
     5221 gtcggtcagg ttgcgctgga cgatgacgac gaccgcgacg gccacggcga agtaggcgac
     5281 ggcgacgacc gcggtcgccg cgagggcgac ccggatcgag gtccgccgga cgctcggcag
     5341 ggcggagact gcgctcacgc gtcctcgagc cggtagcccg ccccgcggac ggtgacgatg
     5401 cggatcccgg cgttcggcag ctttgcccgg atccggctga gctggacgtc gatggcattc
     5461 gagccgagcg gctcggtctc gtccgcccag ccgtgctccg cgatcgcctt gcgatcgacg
     5521 accgccggcg cgcgacgcat caggagctcg aggatccggt actcggtcga ggtgaggccg
     5581 agctcgcgac cgtcgaccgt gacgacccgg gtgacgggat cgagaacgag ccggccgcgc
     5641 tcgatgacgg gcccatcgac gccgcgcggc cggcgttgga gggcccggac gcgggcgagc
     5701 agctcgccga agtcgaacgg cttgacgaga tagtcgtcgg cgccgctgtc gagaccgcgg
     5761 atccgatcgg ccggcgtgtc acgggccgtc agcaggagga tcgcggtcgg gcggcggttg
     5821 cgccgggccc actcgacgac ctcgatcccc tcggcaccgg gcatccgcca gtcgaggatc
     5881 gcgacgtcgt agtcgtagaa cttcagctgg tcgatcgcgt cgtcaccgcg atcgacggca
     5941 tcgacgtggt agccgctgtc ggcgaggccg aggacgatga cgtcccggag ccccgggtcg
     6001 tcctcggcga cgaggatccg catggcgtca gcgtcgcacg gatggggacc ggttgccgag
     6061 ccatccggca gcgccgcgct ccgggggcgc gatcgggacc cgggcgaggc ggagggccag
     6121 gaccgggcag acggcgacgg cccgcttggc atggtcgagg agggcgtccg gcacgccgcc
     6181 cgcggcgacg atcgggtagc cccagtcatc gagctcgatc agttccggca gcagctcggc
     6241 gcacatgccg aagccgtcgc aggcgatccg atcgacgacc aggtcggttc gcatcgtcgc
     6301 gggttcggag gtgccgacgc gggcggtccg cgcggagcgg aac
//
LOCUS       DATAIW010000350         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_171809, whole genome shotgun sequence.
ACCESSION   DATAIW010000350 DATAIW010000000
VERSION     DATAIW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171809"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /gene="mraW"
                     /locus_tag="VHM48_10675"
     CDS             <1..503
                     /gene="mraW"
                     /locus_tag="VHM48_10675"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX2755920.1"
                     /translation="VGPLQALRRGAQANRIANAIVAARSTAPVGSAEELAALIERVAP
                     GNPRVRRRIHPATRVFQALRIAVNAELDALQDALAASIDLLRPGGRLVVLSYHSLEDR
                     IVKRFFAAERRGCVCPPEVPICVCGKNPRLRLLTRPSLTPTADEIAANPRARSARLRA
                     AERLAA"
     gene            541..738
                     /locus_tag="VHM48_10680"
     CDS             541..738
                     /locus_tag="VHM48_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755921.1"
                     /translation="MSKRHQSSRRRSYGRRQHEVHEREGNRRHNPTEEMELDSFGVAS
                     QADPFAFLDPRAPRIRFAIGE"
     gene            739..1119
                     /locus_tag="VHM48_10685"
     CDS             739..1119
                     /locus_tag="VHM48_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755922.1"
                     /translation="MAVYSGARPRSSFLPQRSRVIDPPTLPRRRIRGAVRARRQTNRL
                     AFVLGGIVLAFLLAFFSLAQTVRVSATGYDLERLGRERDALLVRKQELTTDLNRLGQE
                     PAVRKLAIDAGMSQLAAPIVVGAP"
     gene            1136..2968
                     /locus_tag="VHM48_10690"
     CDS             1136..2968
                     /locus_tag="VHM48_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2755923.1"
                     /translation="MGRTDSRGRALILLIAFAVVGSALFARLAYWQVGQRDRLADEAF
                     QQTTIREETPSRRGDIYDRSGVVVLATTIERERLIANPSEISAARRAAVADAIVSILG
                     LTGADADDLVARMTSAKKYVVLAHALDTTTADRIRAAVASKQIAAVALEPEPVRVYPQ
                     AGGGPNTTLAAKILGFVNREGAGQYGVEQFYQDSLAGRPKVVRAQRDIASRSIPDTTL
                     VKDAGTPGEDLRLTIDAGLQLSLEQELLAAWAADRAKSVSAVVIDPYSGEVYAEASYP
                     SYDGNDYAAIAASDPARFIDPVVSASYEPGSVFKMLTVVAAMEKHQVTTRTRVNDTGI
                     LVLDHGRTHIDDADHKAKGIMTFEDAIAWSRNVVAAKTAIGLDKNLRTASAILHSAWT
                     RFGFGKPTGVDVSGEVGGIVHDPAINAWRQIDLANGAFGQGVAVTPLQLASAYSTMVN
                     GGRTIQPHVVKAIGIVERPSTVGAQVIDADLSHTLVGMMRHVVSTVPFYRDRTLIPGY
                     DVGGKTGTAQIWNPKADHGRGAWKHNIFNYSFIGYIGRETGRPELVIAIRIEEGKPTV
                     AKLGQLEMPVMSFELFRRIAHDAITTPDLVPERPGPADSTTATR"
     gene            2995..>4008
                     /gene="murF"
                     /locus_tag="VHM48_10695"
     CDS             2995..>4008
                     /gene="murF"
                     /locus_tag="VHM48_10695"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX2755924.1"
                     /translation="MTDRRDLSRPTDGVLDADAGLTADELAAITGGTLLTRSERRILG
                     GAVDSRAVEPGNLFVALPGERTDGHDFVGAALDAGAAGVLVGRAPASADLAGRDATVV
                     VVDDPLRALQAVATAWRVRFDPLVVGITGSIAKTSTKEAVAAVLGAAMPTLRNEGNLN
                     NEIGLPLTVLRLRAHHRAAVLEMGMYVGGEIADLARIGRPEIGVVTAVQPVHLSRIGT
                     IDAVEQAKGELVEALPGDGVAILNADDERVRRMAARTGARSMTYGFAADADVRAEQVE
                     SRGADGMAFDLVTDVGRRPVVIPALGRLAVHNALAGAAVGLAAGVPLATIDAGLTGGW
                     SAPH"
BASE COUNT          582 a         1494 c         1381 g          551 t
ORIGIN      
        1 ctgtcggccc tcttcaagcg ctacggcgag gagcccaggc caaccggatc gcgaacgcga
       61 tcgtcgcggc tcgctcgacc gccccggtcg gcagcgccga ggagctggcc gccctgatcg
      121 agcgcgtggc ccccggcaac ccccgcgtcc ggcgccggat ccatccggcg acccgggtct
      181 tccaggcgct ccggatcgcg gtcaacgccg agctcgacgc tctccaggac gccctcgccg
      241 cgtcgatcga cctgctccgg ccgggcggcc gcctcgtcgt cctgagctac cactcgctgg
      301 aggaccggat cgtcaagcgc ttcttcgccg ccgagcggcg cggctgcgtc tgcccgcccg
      361 aggttccgat ctgcgtctgc gggaagaacc cgaggctgcg gctgctgacc cggccgtcgc
      421 tgaccccgac ggccgacgag atcgcagcca accctcgtgc ccggagcgcc cgcctgcggg
      481 ctgccgagcg cctcgctgcc tgacccatcc atcgagccac cgaagagagg aggagtcacc
      541 gtgagcaagc gacaccagtc gagccgtcgc cggtcctatg gacgtcgtca gcacgaggtc
      601 cacgagcggg agggcaaccg ccgccacaac ccgaccgagg agatggagct cgactcgttc
      661 ggtgtcgcgt cccaggccga cccgttcgcg ttcctcgatc cgcgggcgcc gcgcatccga
      721 ttcgcgatcg gcgagtaggt ggccgtctac tcgggggccc gccctcgctc cagctttctg
      781 ccgcagcggt cgcgcgtcat cgatccgccg accctgccgc gacgacggat ccgtggcgcc
      841 gtccgcgccc ggcgacagac gaaccggctg gcgttcgtcc tcggcggcat cgtcctcgca
      901 ttcctgctcg cgttcttctc gctggcccag accgtccgcg tgtcggccac cggctacgac
      961 ctcgaacgcc tcggccgcga gcgcgacgcg ctcctcgtcc gcaagcagga gctgacgacc
     1021 gacctcaacc ggctcggcca ggaaccggcc gtccgcaagc tggcgatcga cgccggcatg
     1081 tcccagctgg cggcgccgat cgtcgtcggc gcgccctgac cgaccggatc gacgaatggg
     1141 ccggaccgac tcgcgtggcc gcgcgctgat cctcctgatc gccttcgcgg tcgtcggatc
     1201 ggccctgttc gcgcgcctgg cgtactggca ggtcggccag cgcgatcgcc tcgccgatga
     1261 ggcgttccag cagacgacga tccgtgagga gacaccgagc cggcgcggcg acatctacga
     1321 tcggtccggc gtggtcgtcc tggcgacgac gatcgagcgc gagcggttga tcgcgaaccc
     1381 gtcagagatc tccgccgccc gacgtgccgc ggtcgccgac gcgatcgtct cgatcctcgg
     1441 actgaccggg gccgacgccg acgacctggt cgcccggatg acgtccgcga agaagtacgt
     1501 cgtcctcgcc catgccctcg acaccacgac cgcggaccgg atccgggcag cggtggcgtc
     1561 gaagcagatc gcggcggtcg ctctcgagcc ggagccggtg cgggtctacc cgcaggccgg
     1621 cggcggtccg aacacgaccc tcgcggccaa gatcctgggc ttcgtcaacc gcgagggagc
     1681 aggtcagtac ggagtcgaac agttctacca ggactcgctg gcgggccggc cgaaggtcgt
     1741 ccgggcccag cgcgacatcg cctcgcgctc gatccccgac acgacccttg tcaaggatgc
     1801 ggggacgccc ggagaggacc tccgcctgac gatcgacgcc ggcctccagc tgtcgctgga
     1861 gcaggagctc ctcgcggcgt gggcggcgga ccgggcgaag tccgtgtcgg ccgtcgtcat
     1921 cgacccgtac agcggcgagg tgtacgccga agcctcgtat ccgtcctacg acggcaacga
     1981 ctacgccgcc atcgcggcga gcgatccggc gcggttcatc gacccggtcg tcagcgcgag
     2041 ctacgagccg ggctcggtgt tcaagatgct gacggtcgtg gcggcgatgg agaagcacca
     2101 ggtcacgacc aggacgcgcg tcaacgacac cggcatcctc gtcctcgatc acggccggac
     2161 ccacatcgac gacgccgacc acaaggcgaa ggggatcatg acgttcgagg acgccatcgc
     2221 ctggtcgcgc aacgtcgtcg cggcaaagac cgccatcggc ctcgacaaga acctccggac
     2281 ggcctcggcg atcctccatt cggcgtggac gcgcttcggg ttcggcaagc cgaccggcgt
     2341 cgacgtctcc ggcgaggttg gcgggatcgt ccacgacccc gcgatcaatg cctggcgcca
     2401 gatcgacctc gccaacggcg ccttcggcca gggcgtggcg gtgacgcccc tccagctcgc
     2461 cagtgcctac tcgacgatgg tcaacggcgg ccggacgatc cagccgcatg tcgtgaaggc
     2521 gatcggcatc gtcgagcgac catcgacggt cggcgcccag gtgatcgacg ccgacctgtc
     2581 gcacacgctc gtcgggatga tgcgccacgt ggtctcgacg gtcccgttct accgcgaccg
     2641 gaccctgatc ccgggctacg acgtcggcgg caagaccggg accgcccaga tctggaaccc
     2701 gaaggccgac cacggtcgcg gggcctggaa gcacaacatc ttcaactact cgttcatcgg
     2761 ctacatcggc cgcgagaccg gccggcccga gctcgtcatc gcgatccgga tcgaggaggg
     2821 caagccgacg gtggccaagc tcggccagct cgagatgccc gtgatgtcgt tcgagctctt
     2881 ccggcggatc gcccacgacg cgatcacgac gccggacctc gtgcccgagc gcccgggccc
     2941 ggccgattcg acgactgcga cccggtgatg gccgccgatg cgacaatggc ccgcgtgacc
     3001 gaccgccgtg acctgtcgcg cccgaccgac ggcgtcctcg atgcggacgc gggcctcacc
     3061 gccgacgagc tggcagcgat caccggtggg acgctgctta cccgctccga acgtcggatc
     3121 ctcggcggcg ctgtcgattc gcgcgcggtc gagcccggca acctcttcgt cgccctgccc
     3181 ggcgagcgga ccgacggcca cgacttcgtc ggcgccgcgc tcgatgccgg cgcggccggc
     3241 gtcctcgtcg gccgggcgcc cgcatctgcc gacctcgccg gacgcgatgc cacggtcgtc
     3301 gtggtcgacg acccgctccg cgccctccag gcggtcgcga cggcctggcg ggtccggttc
     3361 gatccgctcg tcgtcgggat caccgggtcg atcgccaaga cctcgacgaa ggaagcggtc
     3421 gcggccgtcc tcggcgcggc gatgccgacg ctccgcaacg agggcaacct gaacaacgag
     3481 atcggcctgc cgctgaccgt gctccggctg cgcgcccatc atcgggcggc ggtgctcgag
     3541 atggggatgt acgtcggcgg cgagatcgcc gacctggccc ggatcggccg gccggagatc
     3601 ggggtcgtga ccgcggtcca gccggtccac ctgtcgcgga tcgggacgat cgacgcggtc
     3661 gagcaggcca agggcgagct cgtcgaggcg ctccctggcg acggcgtcgc gatcctcaac
     3721 gccgacgacg agcgggtgcg ccggatggcc gcgaggacgg gcgcccgctc catgacctac
     3781 gggttcgcgg ccgatgccga cgtccgcgcc gagcaggttg agtcgcgcgg tgccgacggg
     3841 atggcgttcg acctcgtgac ggatgtcggc cggcggccgg tcgtcatccc cgccctcggc
     3901 cggctggccg tccacaacgc gctggcgggg gcggcggtcg ggctcgccgc gggcgtgccg
     3961 ctcgcgacga tcgacgccgg cctcaccggc ggctggagcg ccccgcac
//
LOCUS       DATAIW010000351         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_128951, whole genome shotgun sequence.
ACCESSION   DATAIW010000351 DATAIW010000000
VERSION     DATAIW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128951"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHM48_10700"
     CDS             <1..282
                     /locus_tag="VHM48_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755925.1"
                     /translation="TGASAAGRREPEADPARRAPNRPLLIGGALTSAALSIAGAAFVA
                     WRLGRPGRASTPMARAARVVRTARLTSIQPGTLRGLRRSSATPAARRFD"
     gene            284..1318
                     /gene="accD"
                     /locus_tag="VHM48_10705"
     CDS             284..1318
                     /gene="accD"
                     /locus_tag="VHM48_10705"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX2755926.1"
                     /translation="MPIKLPSFRPRRDAYPPDLWTKCPSCGEMLFNKQLDKVLRVCPS
                     CGHHFRLSAEARIGMLLDHASFVERDPGLESEDPLGFVDQKTYPDRVAAAQLATGMRD
                     AAVWGTGTIDDRPIVVCVMDFGFMGGSMGAVVGEKVTRAAELALMSRVPLLIVAASGG
                     ARMQEGTLSLMQLAKTIGALERLRAAGVPFLSLLSDPTTGGVFASFAVLGDVNIAEPN
                     ALIGFAGARVSAGTIAQELPPGFQRSEFLFEHGYLDRIIHRSELRSEVSALLRFLVAR
                     PAPIPADGPDGDPLGLPSFRPLSFLSNLAERVLPSESEITGPSNGPSGGLSNGHAAGK
                     PSSPEETPRG"
     gene            1311..2339
                     /locus_tag="VHM48_10710"
     CDS             1311..2339
                     /locus_tag="VHM48_10710"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX2755927.1"
                     /translation="MVDRILGRGRDAAARTQADDAAPSQSDRDPKAETWSRVQLARNL
                     RRPRILDLLPELADEFVELHGDRLFGDDAAVVAGFARIGDRRVAVVGQQKGADTEENI
                     RRNFGMPHPEGYRKAMRVMELAERLGIPIVTFVDVPGAHPGPESEERGIAEAIARSVG
                     LMSRLRTPIVVVITGEGGSGGALAIAVGDVVIALENAVYSVISPEGCASILWRTADEA
                     ATAAVAMKMTAPDQQALGIIDIVIAEPGEGAHTDHAETARRIRTVVVEQLDALAAIPL
                     DALVEARYRRFRALGAYREVEAPPPVIAERPGLADRLRNLLDPGRWSGISSIPAGRRD
                     EPPARDEV"
     gene            2345..3031
                     /locus_tag="VHM48_10715"
     CDS             2345..3031
                     /locus_tag="VHM48_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755928.1"
                     /translation="MTDDRRPLPSAPGSHEPRTAEARAADHAAIDRLAEDLVPALIAK
                     LSASGLGEIEVAEETWTIRVRRPGDATAGHGRRATDRASRSQPGHAGHGHAPGGFEGH
                     RSARDAKSTVGALPGSSNGARPQTTHEAPASGGHRPAESGADPHRVVATSPAVGVFQP
                     RPDVKPGTRVRGGDRLGSVDMLGIPQEVLAPADGILGASLVEAGQAVEYGQELVVIEL
                     AARTADRSEA"
     gene            3031..>3771
                     /locus_tag="VHM48_10720"
     CDS             3031..>3771
                     /locus_tag="VHM48_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2755929.1"
                     /translation="MFKKILIANRGEIALRILRACRTLGVEAVVAYSEADRESLPVQL
                     ADEAICIGPADARRSYLSAPAVISAAIVTGCDAIHPGYGFLSEDDGFAEVVRAHDLTF
                     IGPPANVLERFASKEATRRLLGSLGLPTIPGSNGILRDDAHALAEGERVGYPLLIKPA
                     AGGGGKGMRMVRTPRELEASLNVCRSEARAAFGDDSLYHERWLDETRHVEVQVAVDRY
                     GRGIHLGERDCSVQRRHQKILEEAPTPSL"
BASE COUNT          499 a         1444 c         1303 g          525 t
ORIGIN      
        1 accggcgcgt ccgccgccgg gcgacgtgag ccggaagccg accccgcccg acgcgccccg
       61 aaccggcccc tcctgatcgg cggtgccctc acctcggccg ccctgtccat cgccggcgct
      121 gcgttcgtcg cctggcggct cggccggccc ggtcgggcgt cgacgccgat ggcccgggcg
      181 gcgcgcgtcg tgcgcactgc tcgcctgacg agcatccagc ctggaaccct ccgaggtctg
      241 aggcggagct ccgcgacgcc agcggcccgg agattcgact gaaatgccga tcaagctgcc
      301 gtccttccgg ccccgccgcg acgcctatcc gcccgatctc tggaccaagt gcccgtcgtg
      361 cggcgagatg ctcttcaaca agcagctcga caaggtcctc cgggtctgcc cgtcgtgcgg
      421 ccaccatttc cgcctctcgg ccgaggcgcg gatcgggatg ctcctcgacc acgcgtcgtt
      481 cgtcgagcgc gaccccggcc tggaatcgga ggaccccctc gggttcgtcg accagaagac
      541 ctacccggac cgggtcgccg cggcccagct cgcgaccggg atgcgcgatg ccgccgtctg
      601 gggcaccggg acgatcgacg atcggcccat cgtcgtctgc gtgatggact tcgggttcat
      661 gggtgggtcg atgggcgccg tcgtgggcga gaaggtgacc cgcgccgccg aacttgcgct
      721 catgtcacgg gtcccgctgc tgatcgtcgc ggcgtccggt ggcgcgcgga tgcaggaagg
      781 gacgctgtcg ctcatgcagc tcgccaagac gatcggcgcg ctcgagcggc tgcgggcggc
      841 cggcgtgccg ttcctgagcc tcctcagcga cccgaccacc ggcggcgtct tcgcctcgtt
      901 cgccgtcctc ggcgacgtca acatcgccga gccgaacgcc ctgatcgggt ttgccggtgc
      961 ccgcgtctcg gcgggcacga tcgcccagga gctgccgccc ggcttccagc gctccgaatt
     1021 cctcttcgag cacggctatc tcgaccggat catccatcgc tccgagctcc gttcggaggt
     1081 ctcggcgctc ctccgcttcc tggtcgcgcg cccggcgccc atcccggccg acgggcccga
     1141 cggcgacccg ctcggcctcc cgtccttccg gccgctgtcg ttcctgtcca atctcgccga
     1201 acgtgtgctg ccgagcgagt cggagattac gggcccctcc aacggcccct ccggcggttt
     1261 atccaacggc cacgccgccg gaaagccctc gagtcccgag gagacgcccc gtggttgacc
     1321 ggatcctcgg gcgcggccgc gacgccgctg ctcggacgca ggcggacgac gcggcgcctt
     1381 cgcagagcga tcgcgacccg aaggccgaga cctggtcacg cgtccagctg gcccgcaacc
     1441 tgcgccggcc gcggatcctg gatctcctgc ccgagctggc ggacgagttc gtcgagctcc
     1501 acggcgatcg gctgttcggc gacgacgccg ccgtcgtggc cgggttcgcc cggatcggcg
     1561 atcggcgggt cgcggtcgtc ggccagcaga agggcgccga caccgaggag aacatccggc
     1621 gcaatttcgg gatgccccat cccgagggct accgcaaggc gatgcgggtc atggagctcg
     1681 ccgagcgcct cgggatcccg atcgtgacct tcgtcgacgt tccgggtgcg catccgggcc
     1741 cggagtccga ggagcgcggc atcgccgagg cgatcgcccg ctccgtcggg ttgatgagcc
     1801 gcctccgcac cccgatcgtc gtcgtgatca ccggcgaagg ggggtccggc ggcgcgctgg
     1861 cgatcgcggt cggtgacgtg gtgatcgcgc tcgagaacgc ggtgtactcg gtgatcagcc
     1921 ccgaaggctg cgcatcgatc ctgtggcgga ccgccgacga ggccgcgacc gccgccgtcg
     1981 cgatgaagat gacggcgccc gaccagcagg ccctcgggat catcgacatc gtcatcgccg
     2041 agcccggcga aggtgcccac accgaccatg ccgagaccgc ccgccggatc cggacggtcg
     2101 tcgtcgagca gctcgacgcc ctcgccgcca tcccgctcga cgcgctcgtc gaagcacgct
     2161 accgtcggtt ccgagcgctg ggcgcctatc gcgaggtcga ggcaccgcca ccggtgatcg
     2221 ccgagcgacc ggggctcgcc gaccgtcttc gcaacctgct cgacccgggc cgctggtccg
     2281 gcatctcatc gattcccgcc ggccgtcgcg acgagccgcc ggcccgtgac gaggtctgat
     2341 ccctgtgacc gatgaccgcc gcccgcttcc gtccgcgccg ggatcgcacg aaccgcgcac
     2401 ggccgaggcc cgggcggccg accacgccgc gatcgaccgc ctcgccgagg acctcgtccc
     2461 ggccctgatc gcgaagctgt ccgcgagcgg tctcggggag atcgaggtgg ccgaggagac
     2521 ctggacgatc cgtgtccgcc ggcccggcga cgcaacagcc ggccacggcc gtcgggcgac
     2581 cgaccgtgcg tcgcgctcgc agcccgggca cgccggacac ggccacgcgc cgggcggctt
     2641 cgagggtcac cgcagtgccc gcgacgcgaa gtcgaccgtc ggcgcgctgc cgggctcctc
     2701 gaacggcgcc aggccccaga cgacccacga ggctccggcg agcggcggcc atcgtcccgc
     2761 cgagagcggc gcggatccgc atcgcgtcgt cgcgacgtcg cccgcggtcg gcgtcttcca
     2821 gccgcgaccc gacgtcaagc cgggcacccg cgtccgcggc ggtgaccggc tcgggtccgt
     2881 cgacatgctc ggcatccccc aggaggtcct cgccccggcc gacgggatcc tcggcgccag
     2941 cctcgtcgag gccggtcagg ccgtcgagta cggccaggag ctcgtcgtca tcgagctggc
     3001 ggcccggacg gccgaccgga gcgaggcctg atgttcaaga agatcctcat cgcgaaccgc
     3061 ggcgagatcg cccttcggat cctgcgcgcc tgccggacgc tcggcgtcga ggcggtcgtc
     3121 gcgtacagcg aggcggaccg tgagtcgttg ccggtccagc tggccgacga ggcgatctgc
     3181 atcggcccgg ccgacgcccg ccgttcgtat ctctcagccc ccgcggtcat cagcgccgcg
     3241 atcgtcaccg gctgcgatgc gatccatccg ggctacggct tcctgtccga ggacgacggg
     3301 ttcgccgagg tcgtccgggc ccacgacctg acgttcatcg gcccgccggc caacgtcctc
     3361 gagcggttcg ccagcaagga ggcgacgcgc cgcctgctcg gctcactcgg cctgccgacg
     3421 atccccggct cgaacgggat cctccgcgac gacgcccacg ccctcgccga gggggagcgg
     3481 gtcggctacc cgctcctgat caagccggct gcgggcggcg gtgggaaggg catgcggatg
     3541 gtccggacgc cgcgcgagct cgaggcctcg ctcaacgtct gccgctccga ggcgcgggcc
     3601 gcattcggcg acgattcgct ctaccacgag cgctggctcg acgagacgcg ccatgtcgag
     3661 gtccaggtcg cggtcgaccg ctacggccga ggcatccacc tcggcgaacg cgactgcagc
     3721 gtccagcgcc gtcaccagaa gatcctcgag gaggccccga cgccgtcgct c
//
LOCUS       DATAIW010000352         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_52555, whole genome shotgun sequence.
ACCESSION   DATAIW010000352 DATAIW010000000
VERSION     DATAIW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52555"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /gene="gltA"
                     /locus_tag="VHM48_10725"
     CDS             <1..1441
                     /gene="gltA"
                     /locus_tag="VHM48_10725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX2755930.1"
                     /translation="AKERMAIDRVVMPEQDATARAGNFREVNLGLTYQLAMLEAERCL
                     QCPKPYCVGGCPVAVNIPRFIKLLREGDLPAAAESLLDDNALPCVTGRVCPQESQCEG
                     VCLRAKKGKSVAIGSLERFVADWAQKNPDSVTHTKPARNGKSVAIVGSGPAGLTAAGE
                     LAKVGYEVTIFEAFHAPGGVLIYGIPEFRLPKDIVQAEVERLRDAGVVIEPNSIIGKT
                     FTLAELRERFDAIFVAVGAGLPVFMDVPGENFKGVYSANEYLTRVNLMGAWNEDTNTP
                     VLHGQRVVVVGGGNVAMDSVRTARRLGAAEATIVYRRGAEELPARAEEAHHAHDEGVI
                     FDYLAAPVEVLGTEDRWVRAIRCIRMELGEPDASGRRRPTPIPGSEFEIPCDIVVVAI
                     GTKSNPLLTSTAPELTINEWGYITTDEFGMTSMPGVFAGGDIVRGAATVILAMGDGKR
                     AAHSIDAWLRGAYPPADEVSAAEALARPA"
     gene            1566..4658
                     /locus_tag="VHM48_10730"
     CDS             1566..4658
                     /locus_tag="VHM48_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2755931.1"
                     /translation="MARPTRRDRLSVAEATATRTLTSTTTVPPDQSADGPRRNAAQPF
                     DTAAAWIESPTSVRHLFDEIDLLAVALDRDGRILFVNRALAAATGLEASAVVGRPWFE
                     VLAPAPERARLAALSAQFEDPHDPIRYTSGLITPAGIRTVEWTTAPLRDPADRIVGSM
                     AIGVDLTASAAARAARGRLEVAVEATADAVIITDPDGRITFVNPAFERVTGWRRDEAI
                     GANPNILHSGRQSKAFYTAMWATLKSGRTWHGELVNKRRDGTLFTEEATITPVLATDG
                     SLLQYIGVKRDVTVARAWQSHLDQPAIAQATMSAMIADLVPGTTPESTAEAICDRVLT
                     LPGIVFAGLALFSHGDRVHSLAAVALDRVPIRQVDPTGPWSASDRRRARTLLRLAQAG
                     PSIERRMPDVVSPMSDTFARLGVTALAEAPITSLRRTIGFLQVGAIGADGESRLRASL
                     AALGAFGAVASVLLGPQLGARAQTEERQGTIRRLIDSRAFRPVFQPIVALASGATVGF
                     EALTRFSDGTPPDAQFAAAVSSGLGVQLELATLEVALAASTTLPRDAWLAVNVSAALL
                     EDPARLAYLLWKAGSRSIVLEITEHEAIEDYTRLRCQIDQIGLPVRLAVDDAGAGYSS
                     LRHILELRPDIVKLDRSLVVDIDGDPVRQGLVAGLRHFASIARATVVAEGIETRAEYA
                     VLVDLGIELGQGYLLGKPGPAREVRNHQFKASERRATQVRSGAAAADSPDIVHRVNAI
                     LWEADGTDERMTFVSEGAASVTGHAPDRWLKERRFWENHIHPDDRPAMLDAMGDAMHS
                     AEGVSLEYRFRMSDGTYRWFEDLVEILPGDGGHTRVVGVMIDVTDRRRLEAQLALRAT
                     HDSLTGLLNREGFQQVLVQSGLLHEGGYSAVIFLDLDEFKIVNDSLGHRAGDELLQLI
                     GGRLRGAVRDSDAVARFGGDEFIVFATAMDERALIDLAQRLVDVIAEPSDIAGRLMAH
                     PVSAGIAFVPPGEPPEQAIRNADLAMYVAKRCGGQRLRIFDPSMHQAAIDRFDRSSAT
                     "
     gene            complement(4784..>5330)
                     /locus_tag="VHM48_10735"
     CDS             complement(4784..>5330)
                     /locus_tag="VHM48_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2755932.1"
                     /translation="WKELEGVHNRGDFDLGSHAKASGENLEYFDQAADEHFIPWVVET
                     AAGADRAAFTFLIDAYREEEVRAETRVVLGFHPDLAPYKVAVLPLLKKRPEIVSICHA
                     LRADLATDMMAVYDDTAAIGKLYRRQDEIGTPWCITVDVDSLDDNAVTIRDRDTMTQE
                     RVPLEGVKRLIVDKLAAARAV"
BASE COUNT          817 a         1901 c         1787 g          825 t
ORIGIN      
        1 cgccaaggag cggatggcga tcgatcgggt cgtcatgccc gagcaggacg ccaccgcccg
       61 ggcgggcaac ttccgcgagg tcaacctcgg gctgacctac cagctcgcga tgctcgaagc
      121 cgagcgctgc ctccagtgcc cgaagcccta ctgcgtcggc ggctgccccg tcgcggtcaa
      181 catcccgcgc ttcatcaagc tcctccggga gggcgacctg cccgccgccg ccgaatcgct
      241 tctcgacgac aacgccctgc cctgcgtgac cggccgggtc tgcccccagg agagccagtg
      301 cgagggcgtc tgcctccgcg ccaagaaggg caagtcggtc gcgatcggca gcctcgagcg
      361 gttcgtcgcg gactgggcgc agaagaaccc agacagtgtg acccacacga agccggcccg
      421 gaacggcaag tcggtcgcga tcgtcggctc cggaccggcc ggcctgacgg ctgccggcga
      481 gctggcgaag gtcggctacg aggtcacgat cttcgaggcc tttcacgccc ccggcggcgt
      541 cctcatctac ggcatcccgg aattccgcct gccgaaggac atcgtccagg ccgaggtcga
      601 gcgtctgcgt gacgccggcg tcgtgatcga gccgaactcg atcatcggca agaccttcac
      661 gctggccgag ctgcgggagc gtttcgacgc catcttcgtc gccgtcggcg ccgggctgcc
      721 ggtcttcatg gacgtcccgg gcgagaactt caagggcgtc tactcggcca acgagtacct
      781 gacccgggtc aacctgatgg gcgcctggaa cgaggacacc aacacacccg tcctccacgg
      841 ccagcgggtg gtcgtcgtcg gcggcgggaa cgtcgcgatg gacagcgtcc ggacggcccg
      901 ccggctcggc gccgccgagg cgacgatcgt ctaccgccgc ggcgccgagg agctgccggc
      961 ccgggccgag gaggcccacc acgctcacga cgagggcgtc atcttcgact atctcgcggc
     1021 gccggtcgag gtgctcggca ccgaggaccg ctgggttcgg gcgatccgct gcatccggat
     1081 ggagcttggc gagcccgacg cgtcgggtcg acgccggccc accccgatcc cgggctcgga
     1141 gttcgagatc ccgtgcgaca tcgtcgtcgt cgcgatcgga acgaagtcca atcccctgct
     1201 cacctcgacc gcgcccgagt tgacgatcaa cgagtggggc tacatcacga ccgacgagtt
     1261 cgggatgacg agcatgcccg gtgtcttcgc cggcggcgac atcgtccgcg gcgccgcgac
     1321 cgtcatcctg gccatgggtg acggcaagcg cgcggcccat tcgatcgatg cctggctccg
     1381 cggcgcatat ccgccggcgg acgaggtgag cgcggccgag gcgctggcac ggcccgcctg
     1441 accactgcgc aggtgtacgt acccgggggg tgacgttcga cgcatagccg cgtcctgcat
     1501 cctgggcgaa ggttgccgtg aacgagacgg cctgcccgtc gttcgcagat ccgaccgagg
     1561 cccacatggc acgccccacc cgtcgcgacc gcctgagcgt ggcggaagcg acggccacac
     1621 gcaccctgac gtcgacgacc acggttccgc ccgatcaatc agctgatggg ccgcgtcgaa
     1681 atgccgccca gcccttcgac acggccgccg cctggatcga gtcgccgacg agcgttcgcc
     1741 acctcttcga cgagatcgat cttctcgccg ttgccctcga tcgagacgga cggatcctgt
     1801 tcgtgaaccg ggcgcttgcc gccgccaccg gcctcgaggc atcggcggtc gtcggtcgac
     1861 cctggttcga ggtgctggca ccggccccgg agcgcgcccg attggcggcg ctctcggcgc
     1921 aattcgagga cccgcacgat ccgatccgct acacgagcgg cttgatcacc ccggccggca
     1981 tccgcaccgt ggaatggacg accgcgccgc tccgcgatcc ggcggaccgg atcgtcggat
     2041 ccatggccat cggggtcgat ctgaccgcga gcgccgcggc acgagccgct cgcggccgtc
     2101 tcgaagtcgc cgtcgaagca acggccgacg ccgtgatcat cactgacccc gacggacgaa
     2161 tcaccttcgt caatccggcg ttcgagcggg tcaccggttg gcggcgagac gaggcgatcg
     2221 gcgcgaatcc gaacatcctc cactcgggcc gccaatcgaa ggcgttctac accgcgatgt
     2281 gggcgaccct gaagtcgggc cggacgtggc acggcgagct cgtcaacaag cgccgggatg
     2341 gaacgctgtt caccgaggag gcgacgatca cgccggtgct cgccaccgac ggatccctgc
     2401 tgcagtacat cggggtcaag cgtgatgtca cggttgcgcg agcgtggcag tcacacctcg
     2461 atcaaccggc catcgcccag gcgacgatgt ccgccatgat cgcggatctc gtccccggaa
     2521 cgactcccga gtcgaccgcc gaggcgatct gcgatcgggt cctcaccctg ccgggcatcg
     2581 tctttgccgg tctcgccctc ttcagtcacg gcgaccgggt gcactcgctc gcggccgtcg
     2641 ccctcgatcg cgttccgatc cggcaggtgg acccgaccgg accctggtca gcctcggatc
     2701 ggcggcgggc ccggacgctc ctccggctgg cgcaggcggg accgtcgatt gaacggcgga
     2761 tgccggatgt cgtgtcgccg atgtccgata cgttcgcccg actcggagtc accgcactcg
     2821 cggaggcgcc gatcacatcg ctccgccgga cgatcggctt tctccaggtc ggcgccatcg
     2881 gtgctgacgg cgagagcaga ttgcgtgcct ccctggccgc cctcggcgcg ttcggcgccg
     2941 tcgcgtccgt tctgctcggt ccgcagctcg gtgcgcgggc gcagaccgag gaacggcaag
     3001 gcacgatccg cagattgatc gacagtcgag cgttccggcc cgtcttccag ccgatcgtcg
     3061 cgctggcatc gggagccacg gtcgggttcg aggcgctcac gcggttctcc gacggaacgc
     3121 cgccggacgc gcagtttgcc gcagcagtga gctcggggct cggcgtgcag ctggagctcg
     3181 cgacgctcga ggtcgcgctc gccgcatcga cgaccctccc ccgcgatgcc tggctcgccg
     3241 tcaacgtgtc ggccgcgttg ctcgaggacc cggcccgcct cgcctatctc ctctggaagg
     3301 ccggatcgcg atcgatcgtc ctcgagatca ccgagcacga ggcgattgag gactacacgc
     3361 gactccgatg ccagatcgat cagatcgggc tcccggtccg gctggcggtc gacgatgccg
     3421 gggccggcta ctcgtcgctc cggcacatcc tcgagctgcg gccggacatc gtgaagctgg
     3481 accgatcgct cgtcgtcgac atcgatggcg atcctgttcg ccagggcctc gttgccggac
     3541 tgcgccactt cgcctcgatc gcccgagcga cggtcgtggc ggaggggatc gagacccgcg
     3601 ccgagtacgc ggtcctggtc gacctcggga tcgagctcgg acagggctac ctcctcggga
     3661 agccgggccc ggctcgagag gtgcggaacc atcagttcaa ggcgagcgag cgacgggcca
     3721 cccaggtccg atccggtgcc gcggccgccg actcaccgga catcgttcat cgggtgaacg
     3781 ccatcctctg ggaagccgac gggacggacg aacggatgac gttcgtatcg gagggtgccg
     3841 cgagcgtgac cggtcatgct ccggatcgat ggctcaagga gcggcggttc tgggagaacc
     3901 acatccaccc cgacgatcga cccgccatgc tcgacgccat gggcgacgcg atgcacagcg
     3961 ccgaaggcgt gtccctcgag taccgtttcc ggatgtcgga tggaacctac cgctggttcg
     4021 aggacctcgt cgagatcctg cccggcgatg gcggccacac ccgagtcgtc ggcgtcatga
     4081 tcgacgtcac cgaccgtcgc cggctcgaag cgcagctcgc cttgcgcgcc acccacgact
     4141 ccctcaccgg gctcctcaat cgcgagggat tccagcaggt cctcgtccag tccgggctgc
     4201 tccacgaagg cggatactcg gctgtgatct tcctcgatct ggacgaattc aagatcgtga
     4261 atgacagcct cggccatcgc gccggcgacg agctcctgca gctgatcggc ggtcgccttc
     4321 ggggggctgt ccgcgactcg gacgcggtcg cgcgattcgg cggtgacgag ttcatcgtct
     4381 ttgcgacagc catggacgaa cgcgcgctca tcgacctcgc ccagcgcctg gtcgacgtca
     4441 tcgccgagcc gtccgacatt gccgggcggt tgatggcgca tccggtcagc gccggaatcg
     4501 cattcgtccc gcccggtgaa ccgcccgagc aagccatccg gaacgcggac ctggcgatgt
     4561 acgtcgcaaa gcgctgcggc ggccagcgcc tccgcatctt cgatccctcg atgcaccagg
     4621 ccgccatcga tcgcttcgat cggtcctcgg ccacctgaac gagtcgtcag gccggatcga
     4681 agcccgcggt cgctgagtca ccggacccgc cgaaccggtt ggcccggaac gccgcgatcg
     4741 gccaggtcat cctgcgcggc cgcgaggcgc ccgatcagcc ggctcagacc gcccgcgcgg
     4801 ccgccagctt gtcgacgatc aggcgcttga cgccctcgag cgggacccgc tcctgggtca
     4861 tcgtgtcgcg gtcgcggatc gtgaccgcgt tgtcgtcgag ggagtcgaca tcgaccgtga
     4921 tgcaccacgg cgttccgatc tcgtcctggc ggcggtagag cttgccgatc gccgcggtgt
     4981 cgtcgtagac cgccatcatg tcggtcgcga ggtcggcccg gagggcgtga cagatcgaga
     5041 cgatctccgg tcgtttcttg agcagcggca ggaccgcgac cttgtacggc gcgaggtccg
     5101 ggtggaagcc caacaccacg cgggtctcgg cgcggacctc ctcctcgcgg taggcgtcga
     5161 tcaggaacgt gaacgccgcc cggtcggccc cggccgcggt ctcgacgacc cacgggatga
     5221 agtgctcgtc ggccgcctgg tcgaagtact cgaggttctc gccgctggcc ttcgcatgcg
     5281 acccgaggtc gaagtcaccg cggttgtgga cgccctcgag ctccttccag
//
LOCUS       DATAIW010000353         4115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_171915, whole genome shotgun sequence.
ACCESSION   DATAIW010000353 DATAIW010000000
VERSION     DATAIW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171915"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..846)
                     /gene="efp"
                     /locus_tag="VHM48_10740"
     CDS             complement(289..846)
                     /gene="efp"
                     /locus_tag="VHM48_10740"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX2755933.1"
                     /translation="MISTGELKKGVAIELDGELWQILDYHHIKMGRGSAQVKITLRNI
                     KRGQTVERSFQAGTKWPRASMEKRPVQFLYKDDDDYHFMDNDTYDQFLLSGEQLGDAA
                     QYMKDGMTLDRTSYQGETIGVELPVTVDLVVRETEPGFAGDTQTGARKPATTETGLVV
                     TVPIFVEEGDTIRIDTRTGEYQTRV"
     gene            complement(947..2185)
                     /locus_tag="VHM48_10745"
     CDS             complement(947..2185)
                     /locus_tag="VHM48_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2755934.1"
                     /translation="MSADSTRVAGLPQPADFAPPPTADDHARWTVADAAARPHRLARL
                     RARFEGAGIDAYFGLRREHMRYLTGFTLADGEEKVAGTSGQFLVSGDEVLLLADSRYT
                     IQAGREAPDARIVDGPRDLAAGWASLVASLGARRVGVEAALLPHATWRRLEAAAPDVE
                     LVPVEGWIEADRAIKEPSELERVAAACAVADRALAALLSKVRPGATEHDLALELEWLM
                     RTGGAEAVAFEPTCLVGANAALPHGAPSDTAVRSGAVVLFDFGAQVGGYRSDMSRTLF
                     VGEPAERDLAVYQLVARAQAAAIAELEACVEGELALPSGRDLDGVARRLIEADGRWPA
                     YGHGLGHGIGLQTHEGPSLGAWALDSPLPSPTVFSVEPGIYLEGETGVRIEDLILLDA
                     DATRVERLTRFPREPLVVGG"
     gene            complement(2182..3585)
                     /gene="mltG"
                     /locus_tag="VHM48_10750"
     CDS             complement(2182..3585)
                     /gene="mltG"
                     /locus_tag="VHM48_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX2755935.1"
                     /translation="MTIRSGGRARERSQAHLAEHGADDEVWQPEYATDTGRGGRNGGG
                     RNGGGHNGGGGNNGWNGNRRRGIPGILKFLIFALVLGGIVILLLVTALRPLIRSAVVG
                     WATDNPAALGVPFVADLMEEDLGSKLTDPASTDTTQVSFVVVDGETASSIAAKLEQGG
                     FLADRRAFIFLATEQDLTKDLAAGDFILRKSMTPQQVVDALLKPEQVHYIEIALRTGL
                     RLEQITAKLETIDGLTMDPADFYELAKHPTAKLLGDYPWLKAVLPKGASLEGFLWPAT
                     YKVLPDTTAEELIRDMLDGFNGAVGDLMKVPADRKLTFYQVLSLASMVNREAVLDVDR
                     PLIAGVFQNRTNPKLFGNLHLGSDPTIFWINDTLQLAKMDIATWKTYTFWKGPLPELL
                     PANLPDDLAGYNTYTHQGLIPGPICTPTLADIEAALNPDLKYLYFLALPDGSGKTVYA
                     KTKAEHDKLVAKYYPKP"
     gene            complement(3582..>4115)
                     /gene="ruvX"
                     /locus_tag="VHM48_10755"
     CDS             complement(3582..>4115)
                     /gene="ruvX"
                     /locus_tag="VHM48_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX2755936.1"
                     /translation="RWTGQSGTVSRARQRIERAMTRILGLDLGEKRIGVAIADTQGIA
                     MPLTTLRRAATHAEDAIAIARLLADQGATEIVVGLPLEARGNDGAQARITRDWVDAVQ
                     PLLDAPVRYRDERLTSHLAEQRLGPMKRGRSGGPPSRTQRDAYRARVDREAAAIILQD
                     ELDARATKPIQDMEARS"
BASE COUNT          568 a         1391 c         1497 g          659 t
ORIGIN      
        1 gctcaaccgg acccgagctg ctggaagcgc cagcggtcgt cgtcgcccat gtcacggatt
       61 gactctcgcg ctcgcaccgg agatcggtgg ttcgcgggga ggaccaagtg acgaatgact
      121 gcggccgcga ctgccctttc cgattgctcc accgacggtt ggtccggatg gcggaccatt
      181 gggcggctga acaagccacg agggctcgtt cgcgcacgcc cagacgtcgc ttggtccgcc
      241 gggcggacca ctgtccgggc gcgcttgggc gtgggccgcg acggaccctc agacccgggt
      301 ctggtactcg ccggtccggg tgtcgatgcg gatcgtgtcg ccctcctcga cgaagatcgg
      361 gacggtcacc acgaggccgg tctcggtcgt cgcgggcttg cgggcgccgg tctgggtgtc
      421 gccggcgaag ccgggctcgg tctcgcgcac gacgaggtcg acggtcaccg gcagttcgac
      481 cccgatcgtc tcgccctggt agctcgtccg gtcgagcgtc atcccgtcct tcatgtactg
      541 ggcggcatcg cccagctgct cgcccgagag gaggaactgg tcgtaggtgt cgttgtccat
      601 gaagtggtag tcgtcgtcgt ccttgtacag gaactggacc gggcgcttct ccatcgatgc
      661 gcgcggccac ttggtcccgg cctggaagct ccgctccacg gtctggccgc gcttgatgtt
      721 gcgcagcgtg atcttcacct gggccgagcc gcgacccatc ttgatgtggt ggtagtcgag
      781 gatctgccag agctcgccgt cgagctcgat ggcgacgccc ttcttcaatt caccggtgct
      841 gatcatgtcg gcgttggctc ctggggcggc cgccggtgcc gagctgggcg caccgaaacc
      901 ggccgatcgt tctcttgttc gggcgccgat tgtagcggac gggcggtcag ccgccgacga
      961 cgagcggctc gcgcgggaac cgcgtcaggc gctccacccg ggtcgcgtcg gcgtcgagaa
     1021 ggatgaggtc ctcgatccgg acaccggtct cgccctcgag gtagatcccg ggttcgaccg
     1081 agaagaccgt cggcgacggg agcgggctgt cgagggccca ggcgcccagc gatggcccct
     1141 cgtgcgtctg gaggccgatg ccatgaccga gcccgtggcc gtacgcgggc cagcgaccgt
     1201 ccgcctcgat caggcggcgg gcgacgccgt cgaggtcgcg cccggacggc agggccaact
     1261 cgccctcgac gcacgcctcg agctcggcga tcgcggccgc ctgggccctt gcgacgagct
     1321 ggtacaccgc aagatcgcgc tccgcgggtt cgccgacgaa gagcgtccgg gacatgtcgc
     1381 tgcgatagcc gccgacctgg gcaccgaagt cgaacaggac gacggcgccc gatcggaccg
     1441 cggtgtcgga cggggcgccg tgtggcaggg ccgcgttggc tccgacgagg caggtcggct
     1501 cgaacgcgac cgcctcggcg ccgccggttc gcatcagcca ctcgagctcg agggcgaggt
     1561 cgtgttcggt cgcccccggc cggaccttgg aaaggagcgc cgccagggcc cgatccgcga
     1621 ccgcgcaggc agccgcgacc cgctccagct cggacggctc cttgatcgcc cgatcggcct
     1681 cgatccagcc ttcgaccggc accagctcga cgtccggcgc ggccgcctcc aggcgccgcc
     1741 acgttgcgtg cggcaggagc gccgcctcga caccgacccg gcgtgccccg agcgacgcga
     1801 cgagggatgc ccagccggct gcgagatcgc gcggcccgtc gacgatccgc gcgtccggtg
     1861 cctcacgtcc ggcctggatc gtgtagcgcg agtcggcgag gagcaggacc tcgtcccccg
     1921 agacgaggaa ctgcccggac gtcccggcga ccttctcctc gccgtcggcc agggtgaagc
     1981 ccgtcaggta gcgcatgtgc tcgcggcgga gcccgaagta ggcatcgatc cccgctcctt
     2041 cgaagcgggc acgcaggcga gcgagacggt ggggcctcgc ggccgcgtcc gccaccgtcc
     2101 agcgagcgtg atcgtcggcc gtcggcggcg gcgcgaagtc ggcgggctgc ggcaggccag
     2161 cgaccctcgt ggagtcggcg ctcacggctt cgggtagtac ttggccacga gcttgtcgtg
     2221 ctccgccttg gtcttcgcgt agacggtctt gcccgagcca tccggtaacg ccaggaagta
     2281 gaggtacttg aggtcggggt tcaacgccgc ctcgatatcg gccagggtcg gcgtgcagat
     2341 cgggcccggg atcaggccct ggtgggtgta ggtgttgtag ccggcaaggt cgtccggcag
     2401 gttcgccggg agcagctcgg gcagcggacc cttccagaac gtgtaggtct tccaggtcgc
     2461 gatgtccatc ttcgcgagct ggagcgtgtc gttgatccag aagatcgtcg ggtcggagcc
     2521 caggtggagg ttgccgaaca gcttcgggtt cgtccggttc tggaagacac ccgcgatcag
     2581 cggccgatcg acatcgagga cggcctcgcg gttgaccatc gaggcgaggc tcaggacctg
     2641 gtagaaggtc agcttccggt cggcggggac cttcatcagg tcgccaactg caccgttgaa
     2701 gccgtcgagc atgtcgcgga tcagctcctc ggccgtcgtg tccggcagga ccttgtaggt
     2761 cgccggccag aggaagccct cgagcgacgc acccttcggc aggaccgcct tgagccacgg
     2821 gtagtcgccg agcagcttcg ccgtcggatg cttggcgagc tcgtagaaat cggccggatc
     2881 catcgtcagc ccgtcgatcg tctcgagctt ggccgtgatc tgctcgagcc ggaggcccgt
     2941 ccggagggcg atctcgatgt aatggacctg ctcgggcttg aggagcgcgt cgacgacctg
     3001 ctggggcgtc atcgacttgc ggaggatgaa gtcgccggcg gcgaggtcct tggtcaggtc
     3061 ctgctcggtc gcgaggaaga tgaaggcccg acggtcggcg aggaagccgc cctgctcgag
     3121 cttggccgcg atcgacgagg ctgtctcgcc gtcgacgacg acgaacgaga cctgggtcgt
     3181 gtcggtggag gcgggatccg tcagcttcga gccgagatcc tcctccatga ggtcggcgac
     3241 gaacggcacc ccgagcgccg ccgggttgtc ggtcgcccag cccacgaccg cgctccggat
     3301 caacggccga agcgccgtca ccagcaggag gatgacgatt ccgccgagga ccagggcgaa
     3361 gatcaggaac ttcaggatgc ccgggatccc ccgccggcgg ttgccgttcc agccgttgtt
     3421 gccaccaccg ccgttgtgac ctcccccgtt gcggcccccg ccgttgcggc ctccgcgacc
     3481 ggtgtcggtc gcgtactccg gctgccagac ctcgtcatcg gcgccgtgtt cggcgagatg
     3541 ggcctgggag cgctcgcggg ctcgtccccc cgagcggatc gtcatgagcg ggcctccatg
     3601 tcctggatcg gcttggttgc ccgggcgtcg agctcgtcct ggaggatgat cgccgcggcc
     3661 tcgcggtcga cgcgcgctcg atacgcgtcg cgctgggttc ggctcggggg accgccggaa
     3721 cggccgcgct tcatcgggcc gagacgctgt tcggcgagat ggctcgtcag gcgctcgtca
     3781 cggtagcgga ccggagcgtc gagcaacggc tggacggcgt ccacccagtc gcgcgtgatc
     3841 cgggcctggg cgccgtcgtt gccacgggcc tcgaggggca ggccgacgac gatctccgtc
     3901 gcgccctggt cggcgaggag acgggcgatc gcgatcgcgt cctccgcgtg ggtcgccgcg
     3961 cgacggaggg tcgtcagggg catggcgatc ccctgcgtgt cggcgatcgc gacgccgatc
     4021 cgcttctcgc cgaggtcgag cccgaggatc cgggtcatgg cgcgctcgat ccgctggcgg
     4081 gcgcgcgaga cggtgccgga ctggccggtc caccg
//
LOCUS       DATAIW010000354         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_129007, whole genome shotgun sequence.
ACCESSION   DATAIW010000354 DATAIW010000000
VERSION     DATAIW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129007"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VHM48_10760"
     CDS             <1..738
                     /locus_tag="VHM48_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755937.1"
                     /translation="AAVFVDRVLYGGPDQQRLALGALAGATALGALVSGFAVRTFSLR
                     IVTLAGLLLSAATLGLMAGWTPAVPIGTVAVVLGAFGFGFGLTVTPRSTAAVESAGRA
                     SFGMASAIVTVARMIGMAIGVAILTAYGSTTIDRLYDRLFSAANPDGWKDVIPAELRD
                     RPLRDGLVVDALEAWAAREASGILVGMFLVAGVVTIIAIPPGLALGTRTRRLTAGRTA
                     TGAVAAGGAGGSDGLDGEEGLDSTIAL"
     gene            842..1837
                     /locus_tag="VHM48_10765"
     CDS             842..1837
                     /locus_tag="VHM48_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX2755938.1"
                     /translation="MTPTPLGAHKRGSVRIVSCHGDKASESTDLADLPRLLAAPDHRI
                     WVDLAKPDEDQVKTAADALGLHPLIAEDIRERNQRSKVETFDEDVVHIVLFALRYSGE
                     AAAEEIDFVLGKGYLLTVHDGVLASSTAAPLRFGVDHALQKGPDYLFYAIADTIVDGY
                     FPVLDKIGDDIDDLQDRVMEKASDFTLQRLFAMKRELVDLRRATSPAREIFNQLTNRE
                     IATIDPAHIVYYRDVYDHLIRVTDELDNYRDLVSGTLDIYVSIVNNDLSRIMKRLTGV
                     TVILAGIGAVAGIFGMSEAGAAFQGAEAGGFWLVTLGVVLVALGAAAFLRRIDWI"
BASE COUNT          259 a          673 c          636 g          285 t
ORIGIN      
        1 gccgccgtgt tcgtcgaccg cgtcctgtac ggcggcccgg atcagcagcg gctcgccctt
       61 ggtgcgctgg cgggtgcgac ggcgctcggg gcgctcgtgt cggggttcgc ggtccgcacg
      121 ttctcgctcc ggatcgtgac cctcgccggc ctgctgctca gcgcggcgac tctcgggctg
      181 atggcgggct ggaccccggc ggtgccgatc gggacggtcg ccgtcgtcct cggcgcgttc
      241 gggttcgggt tcggcctgac cgtgacgccg cgcagcaccg ccgcggtcga aagcgccggg
      301 cgcgcgtcgt tcgggatggc cagcgccatc gtcacggtgg cccggatgat cgggatggcc
      361 atcggcgtcg cgatcctgac ggcctacgga tcgacgacga tcgaccgcct ctacgatcgc
      421 ctcttctcgg ccgccaatcc ggacggctgg aaggacgtga tcccggccga gctccgcgac
      481 cggccgctcc gcgacgggct cgtcgtcgac gctcttgaag cctgggcggc gagggaggcg
      541 tcggggatcc tcgtcgggat gtttctcgtc gcgggagtcg tgacgatcat cgcgatcccg
      601 ccgggcctcg ccctcggcac ccggacgcgt agactcacgg ccgggcggac cgcgaccggt
      661 gcggtcgcgg cgggcggagc gggaggcagc gacggactcg atggcgaaga aggtctcgat
      721 tcgacgatcg ccctctgacg cggacggacc ccgctcgcat cgagggtccg gtggcgcgcc
      781 gtcccgcggc gacgccctcg agcggacccg ggacccggga tccggcgacg gcgaccccca
      841 cttgacgccg acccccctcg gggcccacaa gcgtggctcc gtccggatcg tctcgtgtca
      901 cggcgacaag gcgagcgagt cgaccgacct cgccgacctg cccaggctgc tcgctgcccc
      961 cgatcaccgg atctgggtcg acctcgccaa acccgacgag gatcaggtca agaccgcggc
     1021 cgacgcgctc ggcctgcacc cgctgatcgc agaggacatc cgcgagcgga accagcgctc
     1081 gaaggtcgag acgttcgacg aggacgtcgt ccacatcgtc ctcttcgccc tccgctactc
     1141 cggcgaggcc gcggccgagg agatcgattt cgtcctgggc aagggctacc tgctcacggt
     1201 ccacgacggc gtcctcgcct cgtcgacggc cgcgccgctc cggttcggcg tcgatcacgc
     1261 gctccagaag ggccccgact acctcttcta cgcgatcgcc gacacgatcg tcgacggcta
     1321 cttcccggtc ctcgacaaga tcggcgacga catcgacgac ctccaggacc gggtgatgga
     1381 gaaggcctcg gatttcacgc tccagcgcct gttcgcgatg aagcgcgagc tcgtcgatct
     1441 ccggcgggcg acatcgccgg ctcgcgagat cttcaaccag ctgaccaacc gcgagatcgc
     1501 gacgatcgac cccgcccaca tcgtctacta ccgcgacgtg tacgaccatc tgatccgggt
     1561 caccgacgag ctcgacaact accgcgacct cgtgtcgggc acgctcgaca tctacgtgtc
     1621 gatcgtcaac aacgacctgt cccggatcat gaagcgcctc accggggtca cggtgatcct
     1681 cgccggcatc ggggcggtcg cggggatctt cgggatgagc gaggcgggcg ccgcgttcca
     1741 gggggccgag gccggcgggt tctggctcgt cactctcggg gtcgtcctgg tcgcgctggg
     1801 cgccgcggcg ttcctccgcc ggatcgactg gatctgagac cccgacgacc gcc
//
LOCUS       DATAIW010000355         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_112463, whole genome shotgun sequence.
ACCESSION   DATAIW010000355 DATAIW010000000
VERSION     DATAIW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112463"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..226)
                     /locus_tag="VHM48_10770"
     CDS             complement(14..226)
                     /locus_tag="VHM48_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755939.1"
                     /translation="MARARCSSAIGRERDRAELQLVVEAANRTIAILNNEAPTDRQHR
                     RLLDLERELASLARAHEGGDARDGAP"
     gene            complement(278..505)
                     /locus_tag="VHM48_10775"
     CDS             complement(278..505)
                     /locus_tag="VHM48_10775"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX2755940.1"
                     /translation="MSESSDPRDQASRGDPGQPPTRKALIDQRIREAQEAGAFDDLPF
                     RGDRIPLADDSAAGAWALAHHILKNANMVPP"
     gene            644..2014
                     /locus_tag="VHM48_10780"
     CDS             644..2014
                     /locus_tag="VHM48_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755941.1"
                     /translation="MSITIRAVGHAGRRGATRRVPNAAALLGTIDEWLQGATGDALHG
                     HVPGARTPDGAIEIVLHPAARPIRIEATDLGEVLASAMTVPVGPGYHTYVASLLDRMG
                     DELGIAWAKLATPPPGAAGRVAATSPSAAVAAVDPAASIDPTGAFGSGDRHDAERGHL
                     GWLRSALQAVRDARRQGATGLHLATPPGTRFTFDGAVATVLGPRDDAWLERALGDPRV
                     AADVWPWVADAMDARYLLGRALSLLWLEVRWRPPIGVDEVKLVDEVLTTLRRAYPLEP
                     GLAWPWQEWRELFQLRGQPDPATRALLDRGAGPLVGGTAAEGHVPIGYRRRPVTIIHE
                     GWALEVPGSFTSHRTSEEWLGGEAGRSITLAGTETAEGGRPMTADRFLQEVAGHLGVD
                     AMEHDDGVVRGRAKLASDPSSGVEVATVEGFSAVRGRGAAIRIEIDDPQDWKWALDTW
                     RALRPA"
     gene            2116..>4102
                     /locus_tag="VHM48_10785"
     CDS             2116..>4102
                     /locus_tag="VHM48_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX2755942.1"
                     /translation="MVDDEIIVSATDLVGFLECGHLTTLELGRLDGLWDKPHQREDPE
                     VVLLQERGDAHELAYLERLTSEGRTVHLGTKEELTTPDRLQAAEADTIAAMRRGVDVI
                     YQATLFDGRWRGHADFLLRVDGPSDLGDWHYEVADTKLARSVKGSALLQVCVYSDRLA
                     TVQGRRPERIHVVTGDSETHTLRLDDFAAFYRAVKLRFEDRVFGDGRHDASAALTYPD
                     PVDHCRVCVWFPTCIDRRRSDDHPSIVAGMTRAATQRLQEAGIPTRRTLAVLDPVTGV
                     PDLNPRTLGRLREQARIQVAGEDQRALLCELIEPIAEEPGRGLALLPEPAPLDLFFDI
                     EADPWIENGGLEYLLGLVELVGGSPLYRPLWGHDRAGEREAFEAFIDLVIDRLRRDPG
                     MHIYHYAGYEAGAIKRLMQRHGTRVDEVDRLLRGGVLVDLYNVVRQGIRASVESYSIK
                     KIEKFYLGAREGPVTEAGFSVVAYETWLRDGDGQHLTDLADYNRDDCVSTWMLRDWLE
                     DRRREAIDRGWPMDRPTIQDGLPSVAQTAQQAETARRVDALTADVPADRSLASDEQRS
                     RWLLAQLLDWHGRDAKPEWWNFYRLNELGVDDLIESGEGLGGLVFEADLEERGRGGRV
                     RRYRFTPQDHRIRVGRPAIEPDGESGLDGGEVIAIDDI"
BASE COUNT          583 a         1465 c         1468 g          586 t
ORIGIN      
        1 gacgaggcga cgatcagggc gcgccgtctc gcgcatcgcc gccctcgtgg gcacgggcca
       61 ggctcgcgag ctcccgctcg aggtccaaca gccgtcgatg ctggcggtcc gtcggcgcct
      121 cgttgttgag gatcgcgatc gtccggttgg cggcctcgac gacgagctgc agctcggccc
      181 ggtcgcgctc ccggccgatc gcgctcgagc accgggcccg cgccacgatc tcatcccttc
      241 gggccagcag gtcgcagacc tcgcgatcgg tcgcgatcta gggcgggacc atgttggcgt
      301 tcttcaggat gtggtgggcc agcgcccacg ccccggcggc gctgtcgtcc gcgagcggga
      361 tccgatcgcc gcggaacggc aggtcgtcga acgcgccggc ttcctgggcc tcccggatcc
      421 gctgatcgat gagcgcctta cgggtcggcg gctgccccgg atctccgcgg ctggcctggt
      481 cgcgaggatc ggaggactcg ctcacgggcg gagaatagcg gacgtccgac gcagcgtgtg
      541 accggcgggg tgggacgatc ggcaggtcgc gatcccttcc acggacatca gcgagcaggt
      601 atcgtcggac gcaggagcgc cagtccatcg gggaggtcac gcgttgtcga tcacgatccg
      661 ggccgtcggc cacgccggcc gacgcggggc gacccgtcgc gtcccgaatg cggccgccct
      721 gctgggcacg atcgacgaat ggctccaggg cgcaacgggc gacgccctcc acggccacgt
      781 ccccggggcg cggacgcccg atggtgccat cgagatcgtg ctccatccgg ctgcccgccc
      841 gatccggatc gaggcgaccg acctcggcga ggtcctcgcc agcgcaatga ccgtgccggt
      901 cgggccgggc taccacacgt acgtcgcgag cctgctcgat cggatgggtg acgagctcgg
      961 gatcgcctgg gcgaagctcg cgacgccgcc gcccggcgcc gccggacggg tcgcggccac
     1021 atcaccgtcg gccgcggtcg cggccgtcga tccggccgcc tcgatcgacc ccacgggggc
     1081 cttcggctcc ggcgaccgcc acgatgccga gcgcggccac ctcggctggc tccgatctgc
     1141 cctgcaggcc gttcgcgacg cccgccgcca gggcgcgacg ggccttcatc tcgcgacgcc
     1201 gccgggcacc cggttcacgt tcgacggcgc cgtggccacc gtcctcgggc cgcgcgacga
     1261 tgcctggctc gaacgcgccc tcggcgaccc gcgggtcgcg gccgacgtct ggccctgggt
     1321 cgccgacgcg atggacgccc gctacctgct cgggcgcgcc ctgagcctcc tctggctcga
     1381 ggtccgctgg cggccaccga tcggcgtgga cgaggtcaag ctcgtcgacg aggtcctcac
     1441 cacgctgcgc cgggcatacc cgctcgagcc cggcctggcg tggccctggc aggaatggcg
     1501 agagctcttc cagctccgtg gccagcccga tccggcaaca cgggcgctcc tcgatcgcgg
     1561 ggcgggtccg ttggtcggtg gcacggcggc ggagggtcac gttccgatcg gctaccgtcg
     1621 ccgtcccgtg acgatcatcc acgagggctg ggcgctcgaa gtcccgggct cgttcacgag
     1681 ccaccggacg agcgaggagt ggctgggcgg tgaggccggt cggagcatca ccctggccgg
     1741 aaccgagact gccgagggcg gccgaccgat gaccgccgac cggttcctcc aggaggtggc
     1801 ggggcacctc ggcgtcgatg ccatggagca cgacgacggc gtcgtccggg gccgagcgaa
     1861 gctggcgtcc gatccgagct ccggcgtcga ggtcgcaacg gtcgaggggt tcagcgcggt
     1921 ccgcggccgc ggcgcggcga tccggatcga gatcgacgat ccgcaggact ggaagtgggc
     1981 gctcgacacg tggcgggcac tgcgaccggc ctgaacgagg agcgtggcgg tcaccgcagg
     2041 ccccggtcgt tcggccgcac agccgttcgt gacaggcgag gtggcacagg cgcgggctaa
     2101 ggttgccctg tgcaattggt cgacgacgag atcatcgtca gcgccacgga cctcgtcggc
     2161 ttcctcgagt gcggccatct cacgacgctc gagctcgggc ggctcgacgg cctgtgggac
     2221 aagccgcacc agcgcgagga tcccgaggtc gtcctcctcc aggagcgcgg cgatgcccac
     2281 gagctcgcgt acctggagcg cctgacgtcc gagggccgga ccgttcatct cggcacgaag
     2341 gaagagctga cgacgcccga tcgcctgcag gcagccgagg cggacacgat cgctgcgatg
     2401 cgccgcggtg tcgacgtgat ctaccaggcc accctgttcg acggccgctg gcgcggtcac
     2461 gccgacttcc tgctccgggt cgacgggccg tcggacctcg gcgactggca ctacgaggtc
     2521 gccgacacga agctcgctcg ctcggtcaag ggctcggcgc tgctccaggt ctgcgtctac
     2581 tcggatcggc ttgcgaccgt ccaggggcga cgcccggagc ggatccacgt cgtgaccggc
     2641 gattcggaga cccacaccct ccgcctcgac gacttcgccg cgttctaccg ggccgtcaag
     2701 ctgcggttcg aggatcgggt gttcggcgac ggccgccacg acgcatcggc ggccctgacc
     2761 tacccggatc cggtcgacca ttgccgggtc tgcgtctggt tcccgacctg catcgaccgc
     2821 cgccgcagcg acgatcaccc atcgatcgtg gccggtatga cccgcgccgc cacccagcgg
     2881 ctccaggaag ccggcatccc gacgcgacgg acgctggcgg tcctcgatcc cgtgacgggc
     2941 gtcccggatc tgaacccgcg gacgctgggc cggctccgcg agcaggcccg gattcaggtc
     3001 gcgggtgagg accagcgggc gctcctgtgc gagctgatcg aaccgatcgc ggaggagccg
     3061 ggccgcggcc tggccctgct cccggagccg gcgccgctcg acctgttctt cgacatcgag
     3121 gccgacccgt ggatcgagaa cggcggtctc gagtaccttc tcgggctcgt cgagctggtc
     3181 ggaggatcgc cgctctaccg cccgctctgg ggccacgacc gggcgggcga gagagaggcg
     3241 ttcgaggcct tcatcgatct cgtgatcgat cgcctccgcc gcgatcccgg catgcacatc
     3301 taccactacg ccggctacga agcgggcgcg atcaagcgcc tgatgcagcg ccacgggacg
     3361 cgcgtcgacg aggtcgaccg attgctccgc ggcggggtcc tcgtcgacct ctacaacgtc
     3421 gtccgccagg gaatccgggc gtcggtcgag tcctactcga tcaagaagat cgagaagttc
     3481 tacctcggcg cccgcgaagg cccggtcacc gaggccggct tctcggtcgt cgcctacgag
     3541 acctggctcc gcgacgggga cgggcagcac ctcaccgatc ttgccgacta caaccgcgac
     3601 gactgtgtct cgacctggat gctccgcgac tggctggagg atcgccgacg agaagcgatc
     3661 gatcgtggct ggccgatgga ccgcccgacg atacaggacg gcttgccgag cgtcgcccag
     3721 accgcccagc aggccgagac cgcgcgacgg gtcgatgcgc tcacagccga tgtcccggcc
     3781 gatcggtcgc tcgcgtccga cgaacagcgg agtcgctggc tcctcgccca gctgctcgac
     3841 tggcacggtc gcgacgcgaa gcccgagtgg tggaatttct accggctcaa cgagcttggc
     3901 gtggacgacc tgattgaatc cggcgagggt ctcggcggac tcgtattcga ggcggatctc
     3961 gaggagcgcg gccggggcgg gcgtgtccgt cgctatcgat tcaccccgca ggaccaccgg
     4021 atccgggtcg gccggccggc gatcgagccg gacggcgaga gcggactcga tggcggcgag
     4081 gtgatcgcga tcgacgacat cg
//
LOCUS       DATAIW010000356         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_278224, whole genome shotgun sequence.
ACCESSION   DATAIW010000356 DATAIW010000000
VERSION     DATAIW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278224"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VHM48_10790"
     CDS             <1..283
                     /locus_tag="VHM48_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755943.1"
                     /translation="SDDVIAEGLRRAGRPALGAAWVNRLTWEQYEAAFIAAGFEIRAL
                     SFTTYPLDVPFYRRFEDVLGRYPIADLERGFFQVVLEKSGRSGGMAPGA"
     gene            complement(286..2166)
                     /locus_tag="VHM48_10795"
     CDS             complement(286..2166)
                     /locus_tag="VHM48_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755944.1"
                     /translation="MIILLANTLVALVGSGLLALALPLSRGLRVVACLLIGQAWITAV
                     LLVTGAGLGRLDGASITAVNLVVAAIAWLASRPSTRGRPETAARVRTMIGSALRRAPS
                     LIREQPAVAVVAIVVGGALAWKLIEAVRLPVLDFDGFSYHLVTVDVWLQSGAIGRVPQ
                     RIWSDGYPANGELVTLWLMAFTRSDVLANLTAMSAVPLAMAATAGLARTLGASRSRAI
                     LVGLMIAGTPAVLALSASTYVDIWAMADIAAVACFGAVAVRSRWPDARVPIVLTGVAI
                     GLAVGTKLSMAVPGAAIALAIVLAGAVRVGPGASRLWPAVRNGLLVGLPAVVLGGYWY
                     AKNLLVFGNPFWPFDVGPFHGLGDFRLITQTPDEFVGLPAFVAILRSWFADIGLRSYD
                     YDNRIGGFGVVWSLCLVLAVVGFARSRPALRPYVALVAAAMALTVLTMPMGWWPRYTL
                     FLVVLVGALAAVGLTRLPRRPAAVLVSAVVVLTSWSTLVTTWRSNFAPINDKPALVPV
                     AMVRLMAGDGPLRTDLGLWARCATVTGLPAGSVVAADGFNLIHATVGHELERRALPPI
                     AMSADPAVLYQHLRDAGATHVVLGPASRGLGAAEGRPDLFAALGTTCRDAHVFALVSS
                     GG"
     gene            complement(2298..3350)
                     /locus_tag="VHM48_10800"
     CDS             complement(2298..3350)
                     /locus_tag="VHM48_10800"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HEX2755945.1"
                     /translation="MPTLKSRLGDWQLDHVHAWSDREERIRAEAVQLVDRSTKVATIG
                     SCFAQELAKVMGTVAIQGGMHPGGLFYSTATIRQELERIAGGWPERAADVPWQVDGGL
                     VDPFRDYDTRYPDEAALLASRAEADAAADALFRGARVVVVTLGLIETWRSPTTGNTYR
                     QIPHPAVFPTLGAVFHRLTVAEMLDDLERIRTVVRDHLGAELIVTTSPVPLHTTFTPL
                     DVRVANVESKSRIRAAVSEFVDRHPDVRYFHSYEMVVTAERQSDYFRDDGRHVHRHAV
                     QYIVSRFLDLFGDRTLQLPDVDTSWITPISKTAAIPSSEARPRAAAARPAPASFPRRA
                     ARSLRRRFGALIGAGR"
     gene            3561..>4495
                     /locus_tag="VHM48_10805"
     CDS             3561..>4495
                     /locus_tag="VHM48_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755946.1"
                     /translation="MVLDTTWTPASWPPVPATEARSAAASPGIIGIRDVAERVLSRLD
                     LIAETSAHLDRWAATSGVVEQLTIRDTSFWFYVRLRHWLWLEERILWAAIADAIVREH
                     RPARIVCALGTDDALIDVLRLVTARDGIELDEEQPPEPGPDNAAPAASTAPSRAPKTG
                     FRHSALARLARRVRDRFRGPPPPPPRVVRQQMTAARLVRVHSLVDALAAEPEARLLVV
                     HEHARQRVETPDGPRSMNPYLDPIVDRLRGTSLEPIVLDIRARAGQDAAWERIGAGVD
                     ERMLPADALAIDRTPPPDAMLANAGATASATAPVEV"
BASE COUNT          725 a         1589 c         1590 g          591 t
ORIGIN      
        1 cagcgacgat gtcatcgccg aggggcttcg gcgggccggc cggcccgcgc tcggcgcggc
       61 ctgggtcaat cgtctgacct gggagcagta cgaggcggcg ttcattgctg cagggttcga
      121 gatccgcgct ctgtccttca cgacgtaccc gctcgacgtg ccgttctacc gccggttcga
      181 ggacgtcctg ggccgctatc cgatcgccga tctcgagcgc gggttcttcc aggtcgtgct
      241 ggagaaatcg ggccgatccg ggggcatggc gcccggcgcc tgatcctatc cgccactcga
      301 gaccaacgcg aagacgtgag cgtctcgaca cgtcgtccca agggcggcga acaggtccgg
      361 tcgaccttcg gcggcgccga ggcccctgct ggccggaccg agcacgacat gggtcgcccc
      421 cgcgtctcga aggtgctggt acaggaccgc cggatccgcg ctcatcgcga tcggtgggag
      481 ggcgcgccgc tcgagctcat gaccgaccgt cgcgtggatg aggttgaacc cgtcggcggc
      541 gacgaccgac ccggcgggca gaccggtcac cgtcgcgcat ctcgcccaca ggccgagatc
      601 cgtccggagc gggccgtcgc cagccatcaa ccggaccatg gccacgggga cgagcgccgg
      661 cttgtcattg atcggcgcga agttcgatcg ccaggtcgtg accagggtcg accaggaggt
      721 caacacgacc acggccgaaa cgagaacggc cgcgggtcgg cgcggcagtc gggtcagccc
      781 gacggcggcg agggccccga cgagaacgac gaggaagagc gtgtagcgcg gccaccagcc
      841 catcggcatg gtcaggaccg tgagggccat cgctgctgcg acgagcgcga catacggacg
      901 cagcgctgga cggctccgcg cgaacccgac gaccgccagc accaggcaca acgaccagac
      961 gaccccgaaa ccaccgattc ggttgtcgta gtcgtagctc cggaggccga tgtcggcgaa
     1021 ccacgaccgg aggatcgcca cgaacgcggg caatccgacg aattcgtccg gcgtctgcgt
     1081 gatgagccgg aagtcgccca ggccgtggaa cggaccgaca tcgaacggcc agaacgggtt
     1141 gccgaacacg agcaggttct tggcatacca gtagccaccc aggacaacgg ccggcaggcc
     1201 gacgagcagg ccgttgcgga ccgccggcca cagtcgggag gctcctggcc cgacccggac
     1261 tgcgccggcc aggacgatgg cgagggcgat cgctgccccc ggcacggcca tcgacagctt
     1321 cgtgccgacc gcgagaccga tggcgacgcc ggtcagcacg atggggactc tcgcgtctgg
     1381 ccacctcgac cggacggcaa ccgccccgaa gcaggccacg gccgcgatat cggccatggc
     1441 ccagatgtcg acataggtcg atgcggacag ggccaggacc gccggcgtgc cagcgatcat
     1501 gaggccgacg aggatcgccc ggctgcgcga cgcgcccagc gtccgggcga gcccggccgt
     1561 tgccgccatc gccaggggca ccgcggacat tgcggtcagg ttggcgagga cgtcggacct
     1621 ggtgaacgcc atcaaccaga gcgtgacgag ctcgccgttg gcgggatagc cgtccgacca
     1681 gatgcgttgc ggaactcgtc cgatcgcccc gctctggagc cagacgtcga cggtcacgag
     1741 gtgatacgag aacccgtcga agtcgagaac cggcagccgc accgcctcga tgagcttcca
     1801 cgccagcgct ccgccgacga cgatcgcgac gaccgcgacc gccggctgct cgcgaatgag
     1861 ggacggagcc cgtcggaggg ccgatccgat catcgttcgg acccgcgcgg ccgtctccgg
     1921 tcgcccacgg gtggagggtc ggctggcaag ccaggcgatc gccgcgacga cgagattgac
     1981 ggccgtgatg gacgcgccgt cgagccggcc aaggccggcc ccggtgacga gcaggaccgc
     2041 cgtgatccag gcctgcccga tgagaaggca ggcgacgacc cgcagcccac ggctgagcgg
     2101 gagggcaagc gcgagaagtc cactgccgac cagggcgacg agggtattcg cgagcaggat
     2161 gatcatgccg ccgtcgatcc tacgctgacc ggacgcaatc tcgacccgat cggcgccgcc
     2221 gccgcctggc cccctgatca gctccgggtc ggtcctgctg agcggcgggc gtcagcggtg
     2281 agcgtcagtg gcgaccgtca gcggccggcg ccgatgagcg ctccgaacct ccgacgcagg
     2341 ctccgggcgg cgcgccgcgg gaacgaggcc ggggcgggcc gcgctgccgc ggcgcgcggg
     2401 cgggcctccg acgacgggat cgctgcggtc ttcgagatcg gcgtgatcca ggacgtgtcg
     2461 acatccggca gctggagcgt ccgatcgccg aacaggtcga ggaaccgcga cacgatgtac
     2521 tggacggcgt ggcgatggac atgccggccg tcgtcgcgga agtagtcgct ctggcgctcg
     2581 gcggtcacga ccatctcgta cgagtggaag tagcgcacgt cgggatggcg atcgacgaac
     2641 tccgacacgg cagcccggat ccggctcttc gactccacgt tcgcgacgcg gacgtcgagc
     2701 ggggtgaacg tcgtgtggag cgggaccggg gaggtcgtga cgatcagctc cgcgccgaga
     2761 tggtcacgga cgacggtccg gatccgctcg agatcgtcaa gcatctcggc gacggtcagg
     2821 cgatggaaga ccgcgccaag ggtcgggaac acggccgggt gcgggatctg gcgatacgtg
     2881 ttgcccgtcg tcgggctgcg ccacgtctcg atcaggccca gcgtgaccac gacgaccctg
     2941 gcgccccgga agagcgcatc ggctgcggcg tccgcctcgg ccctgctggc gaggagggcg
     3001 gcctcgtccg ggtagcgggt gtcgtagtcg cggaacgggt cgaccaggcc accatcaacc
     3061 tgccacggca cgtcagcggc tcgctcgggc cagcccccgg cgatccgctc gagctcctgg
     3121 cggatcgtgg ccgtcgagta gaacaggcca ccgggatgca tcccgccctg gatggcaacc
     3181 gtgcccatga ccttcgccag ctcctgggcg aagcagctcc cgatcgtggc gaccttggtc
     3241 gatcggtcga cgagctggac ggcttcggcg cggatccgct cttctcgatc cgaccaggcg
     3301 tggacgtgat cgagctgcca atcgccgagg cgactcttga gggtcggcat ggctgagagg
     3361 ataccgccca ccggcgaaga tgcggcacgc ggccgaagcg agccgatatg cttcgtcgat
     3421 gcggaccgcg atcacgcgcc atcgagatgg tccatcctga cggcggatcc cgtcccggcg
     3481 gcctgatgca tcccgcggcg tcaggcggcg cgatccgatt cacccaggtc ccgccggatc
     3541 ccggcgcggc tgatctggcg gtggtcctcg acacgacgtg gacgcccgcc tcctggccgc
     3601 ccgttccggc gacggaagct cgatcggccg ctgcgtcgcc cggcatcatc ggcatccgcg
     3661 atgtcgccga acgggtcctg tcgcggctcg atctcatcgc cgagacgtcg gcccacctcg
     3721 accgctgggc cgcgacctcc ggcgtcgttg agcagctcac gatccgcgac acgtcgttct
     3781 ggttctacgt ccgcctccgc cactggctgt ggctcgagga gcggatcctg tgggccgcga
     3841 tcgccgacgc aatcgtccgg gagcaccgcc cggcgcggat cgtgtgcgcg ctcggaaccg
     3901 acgacgccct gatcgacgtg ctccgcctcg tcaccgctcg cgacggcatc gagctggacg
     3961 aggagcagcc acccgagccc ggacccgaca acgccgcgcc ggccgcatcg accgccccga
     4021 gtcgtgcgcc gaagaccggt ttccgccatt cggccctggc ccgactcgct cgtcgggtgc
     4081 gagaccggtt ccgcgggcca ccaccgccgc caccgcgcgt cgtccggcag cagatgacgg
     4141 cggcccgcct cgtccgggtc cactccctgg tcgacgccct ggccgccgag cccgaggcac
     4201 gcctgctcgt tgtccacgag cacgctcggc aacgggtcga gacccccgat ggcccacgct
     4261 cgatgaaccc gtacctcgat ccgatcgtcg accgcttgcg gggaacgagc ctggagccga
     4321 tcgtcctcga catccgggcc cgagcggggc aggacgcggc gtgggagcga atcggtgccg
     4381 gcgtggacga gcgcatgctc ccggcggacg cactggcgat cgatcggacg ccgccacccg
     4441 acgccatgct cgccaacgcc ggcgccacgg cctccgccac ggctccggtc gaggt
//
LOCUS       DATAIW010000357         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_188785, whole genome shotgun sequence.
ACCESSION   DATAIW010000357 DATAIW010000000
VERSION     DATAIW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188785"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..696
                     /locus_tag="VHM48_10810"
     CDS             202..696
                     /locus_tag="VHM48_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755947.1"
                     /translation="MSRAPAGWDVDSPVQAEVFVVWLDGDRLLLTGPDGPQPWILQLG
                     ATEHPVEVVDRIVRDVVGPPILVHSTSWRRDRDAVLLSFVVVIDRARVGAMASVPIER
                     AELARSAATAAPRAIAVSAVVEHGLRHLAWLVQDDPVVAAELPTPWLPLLAAYVPEPF
                     RSLG"
     gene            712..909
                     /locus_tag="VHM48_10815"
     CDS             712..909
                     /locus_tag="VHM48_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755948.1"
                     /translation="MDEARQFTNRCACGWEVSGSEDVVVDATIDHGRRIHNMEATRDE
                     VLDALHEAATTTDPVNEAATG"
     gene            1035..1373
                     /locus_tag="VHM48_10820"
     CDS             1035..1373
                     /locus_tag="VHM48_10820"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2755949.1"
                     /translation="MPKPLYQAKAEFFRTLGHPARIRVLELLSERDHAVHELLDRIEI
                     EPSNLSQQLAVLRRAGLITQRRDRGEVLYAVSVPEVRDLLIAARSILLVLIEDQDGLK
                     TDLQPTGGRG"
     gene            1423..>2041
                     /locus_tag="VHM48_10825"
     CDS             1423..>2041
                     /locus_tag="VHM48_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2755950.1"
                     /translation="MALIAALLGLAAVAAVAPPSRVNPRALSLALSALACAVILGLGV
                     TILATGTTVAATAGQVLGFTLIDVRYDALSAVFLVALGGVGVASSLFGIGHDAHDPGP
                     ADRTAAAYPIFLASLALVFGAADAFAFLFAWELMALSSAVLVVGSRPSAEVARAGYVY
                     LAMTHLATAALIVAFAILTAAAGSTSFAGFAAAAGALPPLGRDVVF"
BASE COUNT          260 a          768 c          702 g          311 t
ORIGIN      
        1 ggacggcgcc gaaccagccg accacctccg cgggcgtcgc cagtggcggg ccgacgaggc
       61 ggctgttgcg cagccgggcg tgggcgatcg cgcggttcgt cacctccgga gtctacgagc
      121 cgaggccacc gggaccgaca tgaatcccgt cgacggcggc aacatctgcg gctcgatcgc
      181 cgcgctcgct acgatgacga ggtgagccgc gcacccgccg gttgggatgt cgacagcccg
      241 gtccaggccg aggtcttcgt cgtgtggctc gatggcgatc gactcctcct gaccggcccc
      301 gacggaccgc agccgtggat cctccagctc ggagcgaccg agcacccggt cgaggtcgtc
      361 gaccggatcg tccgcgatgt ggtcggcccg ccgatcctcg tccactcgac ctcgtggcgg
      421 cgcgatcgcg acgcggtgct cctgagcttc gtcgtggtga tcgaccgggc ccgggtcggt
      481 gccatggcca gcgtcccgat cgagcgcgcc gagctggccc ggtccgcggc gaccgccgcg
      541 ccgcgcgcca tcgcggtcag cgccgtcgtc gagcacggcc tgcgccacct cgcgtggctc
      601 gtccaggacg acccggtcgt cgccgcggag ctgccgacgc cgtggctgcc gctgcttgcg
      661 gcctacgtcc ccgaaccgtt ccgctcgctc ggctgaagga gggtcatcgc catggacgaa
      721 gcgcgccagt tcacgaaccg ctgcgcctgc ggctgggaag tcagcgggtc cgaggacgtg
      781 gtcgtcgacg cgacgatcga ccacggccgg cggatccaca acatggaggc gactcgtgac
      841 gaggtgctcg atgcgctcca cgaggcggcc acgacgaccg atccggtgaa cgaggcggcg
      901 accggctgac gaaggccgcg gtcaccgact cgggcgactg agcccgacac caggtggcag
      961 gtccatccgc gggctaacat gaacgttcct tcatgtgatc aagtcgtgga gtcgaggcac
     1021 ctacccgagg ccgtatgccg aagcccctgt accaggccaa ggccgagttc ttccggaccc
     1081 tgggccaccc tgcccggatc cgcgtcctcg agctcttgag cgagcgcgat cacgccgtgc
     1141 acgagctgct cgaccggatc gagatcgagc cgagcaacct cagccagcag ctcgccgtcc
     1201 tccgccgggc cggcctgatc acccagcggc gcgatcgcgg cgaggtgctc tacgccgtca
     1261 gcgtcccgga ggtccgcgat ctcctcatcg ccgcccgttc gatcctgctc gtcctgatcg
     1321 aggaccagga tggcctgaag acggacctcc agccgaccgg cggacgcggg tgatccagcc
     1381 cgtcaccggc gacctggcgg cgttcggcga cgggacacgc ccatggcatt gatcgcggcc
     1441 ctcctcgggc tggcggccgt cgccgccgtc gccccgccga gccgggtcaa cccgcgggcc
     1501 ctgtcgttgg ccctgtccgc cctggcctgc gccgtcatcc tcgggctcgg cgtgacgatc
     1561 ctcgcgaccg ggaccaccgt ggccgcgact gccggacagg tcctcggctt caccctgatc
     1621 gacgtccggt acgacgccct gtcggccgtg ttcctcgtgg ccctcggcgg cgtcggcgtc
     1681 gcgagctcgc tgttcgggat cggccacgac gcccatgacc ccggcccggc cgaccggacg
     1741 gcggccgcct acccgatctt cctcgccagc ctcgccctgg tgttcggtgc ggccgatgcg
     1801 ttcgccttcc tgttcgcctg ggagctgatg gcgctgagct cggcggtcct cgtcgtcggg
     1861 tcacgcccga gcgccgaggt cgcccgcgcg ggctacgtgt atctcgccat gacccacctc
     1921 gcgacggccg ccctgatcgt cgccttcgcg atcctgaccg ccgctgccgg ctcgacgagc
     1981 tttgccgggt tcgcggccgc ggccggtgca ttgccgccgc tcgggcggga cgtcgtgttc
     2041 g
//
LOCUS       DATAIW010000358         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_53055, whole genome shotgun sequence.
ACCESSION   DATAIW010000358 DATAIW010000000
VERSION     DATAIW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53055"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VHM48_10830"
     CDS             <1..550
                     /locus_tag="VHM48_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755951.1"
                     /translation="VLLANELSDRLPADTWGDVTRVAVRATSIGYGLAALIDGDSLDD
                     VFEALRRPPVRLVDDELSHLGVVLVLRRVDGGDQRVVAAHYVRPVARDMHGHVQRLGP
                     AVLATWDPDTDAFEHFGWGVTPELALRVGRRAGDFEIEVDRRREYLAELVATSVTGPN
                     EARSAIRAYRPAPAFDAPGSRG"
     gene            592..2034
                     /locus_tag="VHM48_10835"
     CDS             592..2034
                     /locus_tag="VHM48_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755952.1"
                     /translation="MDPFAQAKPQSTTLAELRSSGWRSRTVKEELRANLLARLASGAQ
                     VFPGVVGYEDSVVPAIENAILAGHDLVFLGERGQAKTRMARLLVGLLDPWLPIVRGGE
                     LNDDPYAPISPAARAIVVEGGDATPIDWLPRDRRYAEKLATPDITIADLIGEVDPIKV
                     AEGRYLSDELTLHYGLIPRANRGIVAINELPDLAERIQVGLLNILEERDVQIRGFVVR
                     LPLDLYMVASANPEDYTSRGRIITPLKDRLGSQIRTHYPRTLEHEMAIVRQEKHRFEA
                     ADGVPTVVVPAFMEELVAELTHLARRSPEISQRSGVSVRVSVANAEVLEAAALKRAIR
                     LGEPVGAPRVSDLGAVVASTVGKVELESAGDDAPEERIVERLITKALHATFGRHVSID
                     ELEGVVEAFEDGFTVETGDRIPSRDYVAWMREVPGLADSVRRLGAFDVTDGAEEPAVV
                     ASAVEFLLEGLHLARRLNKDRVPGGTVYHR"
     gene            2031..>2892
                     /locus_tag="VHM48_10840"
     CDS             2031..>2892
                     /locus_tag="VHM48_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755953.1"
                     /translation="MTAYSPDPRDLGPEAPGDSPGGRAARRGEVADALARASRYSRWD
                     GSQHVAELDADEILDALADDVMAEGDLAEAIRRLMDRGWRTGDPSRGDMPGLRDLLER
                     LERQREELLDRYKLTDVLGDIRREIDEIVTEERTGVERRLDEAARPGASDEDARLRSM
                     LRDAAARRLDQLDALPSDIGQRIRGLQDYDFLEPNARERFDDLVKRLQGQVLDQFVSG
                     MSDAIRSMSPKDLAAIDRDEVRDLLGPDAARDLAALDDLARRLEEAGYLTRDGERLEL
                     TPRGSRRIGQK"
BASE COUNT          422 a         1009 c         1033 g          428 t
ORIGIN      
        1 cgtgctgctc gcgaacgaac tctccgaccg tctgccggcg gacacctggg gcgatgtcac
       61 gagggttgcc gtgcgtgcca cctcgatcgg ctatggcctg gcggccctga tcgacggcga
      121 ctcactggac gatgtcttcg aggcgctccg tcggccgccg gtccggctgg tcgacgacga
      181 gctgtcgcat ctcggcgtcg tcctcgtcct gcggcgcgtc gacggtggcg accaacgggt
      241 cgtcgcggcg cactacgtcc ggcctgtcgc acgcgacatg cacggccatg tccagcggtt
      301 gggtcccgcg gtcctcgcga cgtgggaccc cgatacggac gccttcgagc acttcggctg
      361 gggcgtcaca ccggagctgg cgctgcgcgt tggccgccgg gcgggcgact tcgagatcga
      421 ggtggatcgt cgtcgcgagt atctcgccga gctggtcgcc acgtccgtga ccgggccgaa
      481 cgaggctcgt tccgcgatcc gcgcctaccg cccggctccc gcgtttgacg ctcctgggag
      541 tcgcgggtag gctcgcggtc cgagcccggc ccttcgcagc tggagcgcac cgtggatccc
      601 ttcgcacaag ccaagcccca gtcgaccacc ctggccgagc tgcgatcgtc cgggtggcgg
      661 tcgcgaaccg tcaaggagga gctccgggcg aacctgctgg cccgcctcgc gtctggggcg
      721 caggtcttcc cgggcgtcgt cggctacgag gattcggtcg tccccgcgat cgagaacgcg
      781 atcctggccg ggcacgatct ggtcttcctc ggtgagcgcg ggcaggccaa gacccggatg
      841 gcgcggctcc tcgtcggcct tcttgacccg tggctgccga tcgtccgcgg cggggagctg
      901 aacgacgacc cgtacgcgcc gatcagcccg gccgcgcgcg ccatcgtcgt ggagggcggt
      961 gacgccaccc ccatcgattg gctcccacgc gaccggcgct acgcggagaa gctcgcgacc
     1021 ccggacatca cgatcgccga cctcatcggc gaggtcgacc cgatcaaggt cgcggagggc
     1081 cgctatctat ccgacgagtt gaccctccat tacggactca tcccgcgagc caaccgcggg
     1141 atcgtggcca tcaacgaact gccggacctg gccgaacgca tccaggtggg cctgctcaac
     1201 atcctcgagg agcgcgacgt ccagatccgt ggcttcgtgg tccgcctgcc gctcgacctg
     1261 tacatggtcg cgtcggcgaa cccggaggat tacacgtcgc gcggccggat catcacgccg
     1321 ctcaaggacc ggctggggtc gcagatccgg acccactacc cgcggacgct cgaacacgag
     1381 atggcgatcg tgcgccagga gaaacaccgc ttcgaggcgg cggacggcgt gccgacggtc
     1441 gtcgtgccgg cgttcatgga ggagctcgtc gcggagctga cccaccttgc gcgccgctcg
     1501 ccggagatca gtcagcggtc cggggtgagc gtccgggtct ccgtggccaa cgccgaagtc
     1561 ctggaggcgg cggcactcaa acgggccatc cggctcggtg agccggtcgg ggcgccacgg
     1621 gtgtcggacc tgggcgcggt cgtcgcctcg acggtcggca aggtcgagct cgaatcggcc
     1681 ggcgatgacg cgccggagga gcgcatcgtc gagcgcctca tcaccaaggc gctccatgcc
     1741 accttcggcc ggcacgtgtc catcgacgag ctggagggcg tcgtcgaggc gttcgaggac
     1801 ggcttcacgg tggagaccgg cgatcggatc ccatcacgtg actacgtcgc gtggatgcgc
     1861 gaggttccgg gactggccga ctcggtccgg cggctcggcg ccttcgacgt gaccgatggt
     1921 gcggaggagc cggcggtcgt cgcctccgcc gtcgaattcc tgctcgaagg tctgcacctg
     1981 gcgcgccggc tcaacaagga ccgagtcccg ggcgggacgg tctaccaccg gtgaccgcgt
     2041 actccccgga tccgcgggac ctggggccgg aggcgcccgg agacagtccg ggcggacgcg
     2101 ccgcgcggcg tggcgaggtg gccgatgccc tcgcccgtgc gtcccggtat tcgcgttggg
     2161 acggcagtca gcacgtcgcc gagctcgatg ccgacgagat cctcgatgcg ctcgccgacg
     2221 atgtcatggc cgagggcgac ctggccgagg cgatccgtcg actcatggac cgcggctggc
     2281 ggacgggcga tccgtcgcgc ggcgatatgc ccggccttcg ggatctgctc gagcggcttg
     2341 aacggcaacg cgaggagctc ctcgaccggt acaagttgac cgatgtgctc ggtgacatcc
     2401 ggcgcgagat cgacgagatc gtcacggagg aacggaccgg cgtggagcgc cgactcgacg
     2461 aagcggccag accgggcgcg tccgacgagg acgcccgact gcgctcgatg ctccgggacg
     2521 cggccgctcg caggctcgac cagctcgacg ctctgccgtc ggacatcggg cagcggatcc
     2581 gcggcctgca ggactacgac ttcctcgaac cgaacgcccg cgaacggttc gacgacctcg
     2641 tgaaacgcct ccaggggcag gtcctggacc agttcgtgtc ggggatgtcc gacgcgatcc
     2701 ggtcgatgag tccgaaggac ctcgccgcga tcgatcgaga cgaggtccgc gatctgctcg
     2761 gccccgacgc ggcccgggat ctcgcggcgc tcgacgacct cgcgcggcgg ctcgaggagg
     2821 ccggctacct cacccgcgac ggcgagcggc tcgagctgac cccacgcggc agtcggcgga
     2881 tcggccagaa gg
//
LOCUS       DATAIW010000359         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_172586, whole genome shotgun sequence.
ACCESSION   DATAIW010000359 DATAIW010000000
VERSION     DATAIW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172586"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VHM48_10845"
     CDS             <1..819
                     /locus_tag="VHM48_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2755954.1"
                     /translation="LSRIGTIDAVEQAKGELVEALPGDGVAILNADDERVRRMAARTA
                     ARTMTYGFAADADVRAEQVVSRGADGMAFDLVTDVGRRPVVIPGLGRLAVHNALAGAA
                     VGLAAGVPLATVVAGLTGGWSAPHRGELVRAGGVTIVDDSYNASPGSVEAALELLGGM
                     AGRRIAVLGEMLELGDEHEAGHERVGAAAAAVVDRLVVVGKGAGGIARGAHAAGLEGD
                     AMARVVDREAARAQLLAELRPGDVVLVKASRGIALDLLVDDLVAALGPVWEPAS"
     gene            816..1799
                     /gene="mraY"
                     /locus_tag="VHM48_10850"
     CDS             816..1799
                     /gene="mraY"
                     /locus_tag="VHM48_10850"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2755955.1"
                     /translation="MKIELIQGLLLAFAIVVILMPPYIRLLRATGFTKQIRIEGPDSH
                     QIKHGTPTMGGALLIGVILALYLLLRWPPEGGIYAPLGALAGVGLLGAFDDYLNAKTG
                     EGIKVRQKLLWQTVFAFAFAYLIQRTYNIDQIAVPFVVGAVPISPPIYVLFAAFAIIA
                     ASNGVNITDGLDGLAGGTLVFAFVAFLLVALLNVPAQPNLAFLCALIIGALLGFLWFN
                     VHPAQIFMGDSGSLSLGATLAVIALITGQILVLPLIGLIFVIETASDIIQIGYFKLSG
                     GKRIFRMAPLHHHFELAGWDEEKITLRFWIVGILAGLLGVTLFLASIQKLA"
     gene            1808..>3314
                     /gene="murD"
                     /locus_tag="VHM48_10855"
     CDS             1808..>3314
                     /gene="murD"
                     /locus_tag="VHM48_10855"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2755956.1"
                     /translation="MTTGRPLDLDRLTIEDVRAGAFRGRIVTVLGLARSGIAAARFLH
                     DAGARVRVYDVRGHDELKDAFDQLGDREVEPWIGPRMDRASSWAGAALIVASPSVNEH
                     FPTTDEFMRFALRDLRLRREAGDPTAPPLVSEADLFLRLCPAPTIGVTGTKGKTTTSS
                     LIAAVLAADPSHPVVLGGNIGIPLVERLPELTPEHRVVIELSELQLPTLARGTTVAVY
                     TNVTSDHLDRHGSVAGYRAAKRRLAELVDPEGALVLNADDPVVTAYAGLGTAPSILYR
                     LDRPMAGGVGVVDGWIVAAGVVRLPMAGGGGAATGPGGRIMPVGELAIPGRHNVSNAL
                     AAVTTGLLFGVAPDAIRRAAAEFTGVEHRLEPVGLIDGVRFVNDSQGTQPDAVIAALR
                     AFDGPIVLIAGGRDKGVDLSGLWPVAAERAAAAVLIGESGPDLEAGFRAAGLARTAHA
                     GTLEEAVRIADAIARAALGAGAPAPATVLLSPAAASFDMFVDYAARGRAFKE"
BASE COUNT          414 a         1229 c         1160 g          511 t
ORIGIN      
        1 ctgtcgcgga tcgggacgat cgacgcggtc gagcaggcca agggcgagct cgtcgaggcg
       61 ctccctggcg acggcgtcgc gatcctcaac gccgacgacg agcgggtccg ccggatggcc
      121 gcccggaccg ccgcccggac gatgacctac gggttcgcgg ccgatgccga cgtccgcgcc
      181 gagcaggttg tgtcgcgcgg tgccgacggg atggcgttcg acctcgtgac cgacgtcggt
      241 cggcggcccg tcgtgatccc gggcctcggc cggctggccg tccacaatgc gctggcgggg
      301 gcggcggtcg ggctcgccgc aggcgtgccg ctcgcgacgg tcgtcgccgg cctcaccggc
      361 ggctggagcg ccccgcaccg aggggaactg gtccgcgcgg gcggagtcac gatcgtcgac
      421 gacagctaca acgcctcgcc gggctcggtc gaggcggcgc tcgagttgct gggcgggatg
      481 gcgggccggc ggatcgccgt cctcggcgag atgctcgagc tcggggatga gcacgaggcg
      541 gggcacgagc gggtcggcgc ggcggccgcc gcggtggtcg atcggctcgt cgtcgtcggg
      601 aagggagccg gggggatcgc gcgcggcgca cacgctgccg ggctcgaggg tgacgccatg
      661 gcgcgggttg ttgaccgcga ggcggcccgc gcgcaactcc tcgccgagct tcggcccggt
      721 gatgtcgtcc tcgtcaaggc gtcgcgcggg attgccctcg atctcctggt cgacgacctc
      781 gtggccgccc tcggcccggt ctgggagccc gcctcgtgaa gatcgagctg atccagggcc
      841 tgctcctggc gttcgcgatc gtcgtgatcc tgatgccgcc gtacatccgg ctgctccgag
      901 cgaccggctt taccaagcag atccggatcg agggccccga cagccaccag atcaagcatg
      961 gcaccccgac gatgggcggc gcgctgctca tcggggtcat cctcgccctg tacctgctgc
     1021 tccgctggcc gcccgagggc ggcatctacg ccccgctcgg cgcgctcgcc ggggtcggcc
     1081 tgctcggcgc gttcgacgac tacctgaacg ccaagaccgg cgaggggatc aaggtccgcc
     1141 agaagctcct ctggcagacc gtgttcgcgt tcgcgttcgc ctacctgatc cagcggacct
     1201 acaacatcga ccagatcgcc gttccgttcg tcgtcggtgc cgtccccatc tcaccgccga
     1261 tctacgtgct cttcgccgcg ttcgcgatca tcgcggcgag caacggcgtg aacatcaccg
     1321 acgggctcga cggcctggcc ggcgggacgc tcgtgttcgc cttcgtcgcc ttcctgctcg
     1381 tcgccctgct caacgtcccg gcccagccga acctcgcatt cctgtgcgcc ctgatcatcg
     1441 gggccctgct cggcttcctc tggttcaacg tccatcccgc ccagatcttc atgggcgact
     1501 cgggctcgct gtcgctcggg gcgaccctcg cggtcatcgc cctgatcacc ggccagatcc
     1561 tcgtcctgcc gctgatcggg ctgatcttcg tgatcgagac cgcgtcggac atcatccaga
     1621 tcggctactt caagctgagc ggcgggaagc ggatcttccg gatggcgccg ctccaccacc
     1681 atttcgagct ggccggctgg gacgaggaga agatcacgct ccggttctgg atcgtcggca
     1741 tcctcgccgg gctcctcggg gtcacgctct tcctcgcctc catccagaag ctcgcgtgag
     1801 cacggccatg acgaccggcc ggccgctcga cctcgatcgc ttgacgatcg aggacgtccg
     1861 cgccggcgcg tttcgggggc ggatcgtgac cgtcctcggg ctcgctcggt ccggcatcgc
     1921 tgccgcccgg ttccttcatg acgccggcgc ccgcgttcgg gtctatgacg tgcgcggcca
     1981 cgatgagctc aaggatgcgt tcgaccagct cggggatcgc gaggtcgagc cctggatcgg
     2041 gccccggatg gatcgagcgt cgtcatgggc aggggcggca ctcatcgtcg cctcgccatc
     2101 ggtgaacgaa cacttcccga cgaccgacga gttcatgcgg ttcgcgctcc gcgacctccg
     2161 ccttcggcgc gaggccggcg atccgacggc accaccgctc gtctccgagg ccgacctgtt
     2221 cctgcgcctc tgcccggcac cgacgatcgg ggtcaccggg acgaagggca aaacgacgac
     2281 gtcctcgctg atcgcggcgg tcctcgccgc tgacccgtcc cacccggtcg tgctcggcgg
     2341 gaacatcggg atcccgctcg tggagcgact gccggagctg acccccgagc accgggtcgt
     2401 catcgagctc tccgagctcc agctgccgac cctggcgcgg ggcaccacgg tcgcggtcta
     2461 cacgaatgtc acctccgacc atctcgaccg gcacggctcg gttgccggtt accgggcggc
     2521 caagcgccgg cttgcggagc tcgtcgaccc cgagggcgct ctcgtcctca acgcggacga
     2581 cccggtcgtc accgcctacg ccggtctcgg gacggcgccc tcgatcctgt accgcctcga
     2641 ccggccgatg gccggcgggg tcggcgtcgt cgacggctgg atcgtggccg cgggtgtggt
     2701 ccgcctgccg atggccgggg gcggcggcgc ggcgaccggg cccggcggcc ggatcatgcc
     2761 ggtcggcgag ctggcgatcc ccggccgcca caacgtgtcg aatgcactcg ccgcggtcac
     2821 gaccggcctc ctgttcgggg tcgcccccga cgccatccgc cgggcggccg ccgaattcac
     2881 cggcgtcgaa caccgcctcg agccggtcgg gctgatcgac ggcgtccgct tcgtcaacga
     2941 ctcgcagggc acccagccgg acgcggtcat cgccgccctc cgggccttcg acggcccgat
     3001 cgtgctgatc gccggcggcc gcgacaaagg ggtcgacctg tccgggctct ggccggtcgc
     3061 ggccgagcgt gccgcggccg ccgtcctgat cggcgagagc ggcccggatc tcgaggcggg
     3121 cttccgcgcc gccggcctcg cccggacggc gcacgcaggc acgctcgagg aggccgtccg
     3181 aatcgcggac gcgatcgccc gcgcggcgct cggcgccggc gcacccgccc ccgcgaccgt
     3241 gctgctgagc ccggcggccg cgagcttcga catgttcgtc gactacgccg cccgcggacg
     3301 ggcgttcaaa gagg
//
LOCUS       DATAIW010000360         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_248527, whole genome shotgun sequence.
ACCESSION   DATAIW010000360 DATAIW010000000
VERSION     DATAIW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248527"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHM48_10860"
     CDS             <1..423
                     /locus_tag="VHM48_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755957.1"
                     /translation="MVPVDRGSRQHLKIMQLLAAVDGDGTTPLVECLVNSAGRLRRGM
                     TAIVITPSTDPGWVRPIAALRARGIGSVVVTLDALAADQLERAERKRAGEPVAEPDQA
                     ASDERAKHARAVRHALAEYELKVHTLTPGRSLAEALGS"
     gene            420..2717
                     /locus_tag="VHM48_10865"
     CDS             420..2717
                     /locus_tag="VHM48_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX2755958.1"
                     /translation="MSHSMRARLAAAPAEGWISLFLVAVLAVAVAWSLDDAALVLGQR
                     AWTDFLPWVSLGGVAAGFIGARARWSRPVAHLIGAAFAALIVPLIIGSILDPAGSPAA
                     RYQATAASSLDAVLDFAVRGLPVTRETGHYLLVLGLLCWANGQFAASSVFRHGRPIGP
                     IIVLGTVLVANMSATVHDQIWFLVLFSMAGLFLLTRLHALDERATWVRRRIGDPTTVG
                     SLYLRGGTVFILVAIFGSLALTATARSAPLAGFWDDARPGLVEISQWLQRIIPAAPDA
                     KNIGVPSFGQQVTIGGTWSTSNDPALEILRKAGDNRHVYWRATTYDNFTLFGWSSTDP
                     VDEARPAGTSILESTADSIPETAARTTETFRIVPQSRLFKVAFSPIDPLSIDRDTTLT
                     TSGDGGFFQAIEIAPGVPYTVTASTQELADIGGGVTQNRLRAAGRDYPDEIVAEYTDV
                     PVGAMGRAATKLLADLKARTKQAGADTPFDLATAIVNELQSTRYQYSTNLPGVCDRDS
                     STVECFAVHKIGFCEHYASTMAILLREDGIPTRLVEGFLPGQLDVATGRETIRTGAAH
                     AWVEVYFPGIGWYGFDPTGGLADPEPIPVGSVQPVPSATPRPSLALPSINPGGRIGEI
                     GPRRAGPVATTGGPASGGPLIAVAIVLIAVVLLGAFLAYRRGPRGVTTPDGVYASVAA
                     LARRFGFGPRPTQTTFEYAAALGDILPGVRPELHTVATAKVEVAYGRRELDDDRLHAL
                     RDSYRRLRVGLLRLAFRRRDRPRRR"
     gene            complement(2725..>3358)
                     /locus_tag="VHM48_10870"
     CDS             complement(2725..>3358)
                     /locus_tag="VHM48_10870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2755959.1"
                     /translation="PIPAGPPLAIVHSPLGRATETAAAIGAAAAGPDAFDRPIPLRSD
                     RRFLEIGQGAWEGLLSTEIAAKDGERLAAWRRTPTLAWAPGGERLADVGARVAPGLTD
                     LLAELASAGRPGSIDAPQVAGYRDRVPDQPWTILVGHDGVFKVILLTLFGLPLERFWM
                     WSSDLCGISVIELRAGRPVVRAMNLTEHLASLLDEEAQAETEARARTGAL"
BASE COUNT          447 a         1258 c         1156 g          497 t
ORIGIN      
        1 atggtgccgg tcgaccgggg cagccgccag cacctcaaga tcatgcagct cctggctgcc
       61 gtcgacggcg acggcacgac cccgctggtc gaatgcctgg tcaactcggc gggtcgcctg
      121 cgccggggga tgaccgcgat cgtgatcacc ccctcgaccg acccgggctg ggtccggcca
      181 atcgccgcgc tccgcgcccg cggcatcggg tcggttgtcg tcacgctcga cgcgctcgcc
      241 gccgaccagc tcgaacgggc ggagcgcaag cgggccggcg aaccggtcgc cgaacccgac
      301 caggccgcct ccgacgaacg cgccaagcac gcccgggccg tccgccacgc cctcgccgaa
      361 tacgagctga aggtccacac cctgacgccc ggccgctcgc tcgcggaggc cctggggtca
      421 tgagccactc gatgcgggcg cgcctcgccg ccgccccggc ggaaggctgg atcagcctct
      481 tcctggtcgc cgtcctcgcg gtggccgtgg cgtggtcgct cgacgatgcc gcccttgtcc
      541 tcggccagcg cgcgtggacc gacttcctgc cgtgggtctc gctcggcggg gtcgctgccg
      601 ggttcatcgg cgcgcgggcc cgctggagcc gccccgtggc ccacctgatc ggggcggcgt
      661 tcgctgcgct catcgtgccg ctgatcatcg gctcgatcct cgacccggcc ggctcgcccg
      721 cagcccgcta ccaggcgacc gcggcctcgt cgctcgacgc ggtgctcgac ttcgcggtcc
      781 ggggcctgcc cgtgacgcgc gagaccggcc attacctcct ggtcctcggc ctgctgtgct
      841 gggcgaacgg ccagttcgcg gcctcgagcg tcttccgcca tggtcgcccg atcggcccga
      901 tcatcgtcct cggcacggtc ctcgtcgcca acatgtcggc gaccgtccac gaccagatct
      961 ggttcctcgt cctgttctcg atggccggcc tgtttctcct gacccggctg cacgccctcg
     1021 atgagcgggc aacctgggtc cgtcgccgga tcggcgatcc gacgaccgtc ggttcgctgt
     1081 acctccgcgg cggcaccgtc ttcatcctcg tcgccatctt cggctcactc gcgttgacgg
     1141 cgaccgctcg ctccgcgccg ctcgcgggct tctgggacga cgcccggccc ggcctcgtgg
     1201 agatcagcca gtggctccag cggatcatcc cggcggctcc ggacgccaaa aacatcggcg
     1261 ttccgtcctt cggtcagcag gtgacgatcg gggggacctg gtcgacctcg aacgatccgg
     1321 ccctcgagat cctgcgcaag gcgggcgaca atcgtcatgt ctactggcga gccacgacct
     1381 acgacaactt caccttgttc ggctggtcct cgaccgatcc ggtcgacgaa gcccgaccgg
     1441 ccggcaccag catcctcgag agcacggccg attcgatccc ggagacggcc gcgcggacga
     1501 ccgagacgtt ccggatcgtg ccccagtcca ggctgttcaa ggtcgcgttc agcccgatcg
     1561 atcccctctc gatcgaccgt gacacgacgc tgacgacgtc cggtgatgga ggcttcttcc
     1621 aggcgatcga gatcgcgccg ggggttccct acacggtcac cgcgtcgacc caggagctgg
     1681 ccgatatcgg cggcggcgtg acccagaacc gcctccgggc cgccggtcgg gactacccgg
     1741 acgagatcgt cgcggagtac acggatgttc cggtcggggc gatgggacgt gccgccacca
     1801 agttgctcgc tgacctcaag gcgcggacga agcaggccgg agcggacacg ccattcgacc
     1861 tcgcgaccgc gatcgtcaac gagctccaga gcacccgtta ccagtacagc accaacctcc
     1921 ctggcgtgtg cgaccgcgac agctcgaccg tcgagtgctt cgccgtccac aagatcggct
     1981 tctgcgagca ctacgcgagc acgatggcga tcctgctccg agaggacggg atcccgaccc
     2041 ggctcgtcga gggcttcctg ccgggtcagc tcgacgtcgc gaccggccgc gagacgatcc
     2101 ggaccggggc cgcccatgcc tgggtggagg tgtatttccc gggcatcggc tggtacggct
     2161 tcgacccgac cggcgggctg gccgatcccg agcccatccc cgtcggatct gtccagccgg
     2221 tccccagcgc gacgcctcgg ccgtcgctcg cgcttcccag catcaatccg ggcggccgga
     2281 tcggtgagat cggcccgcgt cgggccggtc cggtggcgac gacgggcggt ccagcgagcg
     2341 gcggccccct gattgcggtc gccattgtgc tcatcgccgt cgtcctgctc ggggcgttcc
     2401 tggcctaccg ccgggggccg cgcggggtca ccacgcccga cggggtctac gcctcggtgg
     2461 cggcgctcgc ccgacgcttc ggtttcggtc cacgcccgac gcaaaccacg ttcgaatacg
     2521 cggcggcgct cggcgacatc ctgccgggtg tccggccgga gctgcacacc gtcgcaacgg
     2581 ccaaggtcga ggtggcgtac ggccgacgcg agctcgacga cgaccggctg cacgccctgc
     2641 gcgactcgta ccggcggttg cgcgtcgggc tgctccggct cgcgttccga cgacgcgacc
     2701 ggccgcggcg gcgctgactc ctcgtcagag cgcgcccgtc cgggctcgag cctccgtctc
     2761 cgcctgcgcc tcctcgtcga gcaggctcgc gagatgctcg gtcagattca tcgcccgaac
     2821 gaccgggcga ccggcccgga gctcgatgac cgagatcccg cacaggtcgg acgaccacat
     2881 ccagaaccgc tcgagcggca gcccgaacaa ggtcaggagg atgaccttga agacgccgtc
     2941 gtggccgacg aggatcgtcc acggctgatc gggcacgcga tcgcgatatc cggcgacctg
     3001 gggggcgtcg atcgagcccg ggcggccggc ggacgcgagc tccgcgagga ggtcggtcag
     3061 gccgggcgcg acccgggctc cgacgtcggc cagccgctct ccgcccggtg cccacgcgag
     3121 ggtcggggtt cgtcgccacg cggccagtcg ctcgccgtcc ttcgcggcga tctcggtcga
     3181 gagcaggccc tcccacgcgc cctggccgat ctccaggaag cgccggtcgg atcggagcgg
     3241 gatcgggcgg tcgaaggcgt cgggcccggc ggcggcggcg ccgatcgcgg cggcagtctc
     3301 ggtcgcccgg ccgaggggcg aatggacgat ggcgagcggc gggcccgcgg ggatgggc
//
LOCUS       DATAIW010000361         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_53344, whole genome shotgun sequence.
ACCESSION   DATAIW010000361 DATAIW010000000
VERSION     DATAIW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53344"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VHM48_10875"
     CDS             <1..250
                     /locus_tag="VHM48_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755960.1"
                     /translation="GDTFGALFRAIYADVLTGRANPDPPYADFAAGHDEMLVNDAIGR
                     SAAESRWVDVDRTSLGGAGLPPAGAASPSFPVLSEVPS"
     gene            247..1161
                     /locus_tag="VHM48_10880"
     CDS             247..1161
                     /locus_tag="VHM48_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2755961.1"
                     /translation="MRLGLLTAPFPNTPLADVVDWTAGHGFESIEIACWPPATGPTRR
                     YAGTSHIDVANLSQGEATDLAAGIRGSGLVISGLGFYPNPLHPDPDHRATVIAHLKHV
                     ITAAEKMDVPLVNTFMGADAAKTQDQNWEDALRIWPDIVSFAADHGRKITIENCPMLF
                     SNDEWPGGHNLATTPRIWRRIIEQWGGTIGLNFDPSHLILQMIDIPRFIREFGPHILH
                     VQAKDLWIDREGLYERGVFSMGMGWQVPRLPGLGDVDWAPLFGALWRAGYDGDVIIEH
                     EDRDFEKTDELVKRGFLLARDVLRPYIK"
     gene            1158..1916
                     /gene="deoC"
                     /locus_tag="VHM48_10885"
     CDS             1158..1916
                     /gene="deoC"
                     /locus_tag="VHM48_10885"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2755962.1"
                     /translation="MSKEHVPFTVDNLTERDLAKAIDHSLLRPELDDAFVEAGCRLAA
                     EYDVASVCVRPVDVHRAVAILDGTDVKVGTVIGFPHGNSTTATKVYEAHRALEDGAVE
                     LDMVIDIGALKSGRDRDVQADIAAVVEVAHAGGAILKVIFENHYLTPDEIVRACRLAE
                     AAGAEFVKTSTGFAASGATHEDLALMRQSVSDHIQVKAAGGVRTLDALIDVMNLGVTR
                     IGATATAAIIDDFRARKAGAGPLAATATAGEAGY"
     gene            1916..2971
                     /locus_tag="VHM48_10890"
     CDS             1916..2971
                     /locus_tag="VHM48_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2755963.1"
                     /translation="MGEVRIGMLGSGFIGVFHADGLRYIRDTRVVANWGAGPERREAF
                     AARFGSRPLATMEAVCADPEVDLVVVSLPNHLHLDAVRAAAANGKGVACTKPLGRNGD
                     EAAEMLRLVTEAGVFHAYLENVVFNAEMIRMREIVESGAIGRLTTFRAREGHSGPHAA
                     HFWDADLAGGGALLDMASHGAEAARAMFGKDLAVRDVFAWGDTLVHGGRTTLEDNAVM
                     LIRFEDGRVATMDVSWSSKGGLESRFEAYGDGGRIVQDIISSPLRAYIERPAGYLGEK
                     VDADTGWVYPVPNETYVHGHDAMMADVVTAFREGRAPTETFEDGLIVNTILDAAYRSI
                     GSGHWESARVPSAASTR"
     gene            2968..>3305
                     /locus_tag="VHM48_10895"
     CDS             2968..>3305
                     /locus_tag="VHM48_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2755964.1"
                     /translation="MTTTSVPGLPWMTAAAALLELVATTQAAAIETASQWSAEAIAAD
                     GLVHLFGTGHSRIPVEEMFPRYGSYPGFNPIVELSMTFHTQVVGANGQRQAMFIERTP
                     GLAEVILSNFS"
BASE COUNT          506 a         1215 c         1100 g          484 t
ORIGIN      
        1 cggcgacacg ttcggcgccc tcttccgggc gatctacgcc gacgtcctca ccggccgcgc
       61 caacccggac ccgccatacg ccgacttcgc cgccggccac gacgagatgc tcgtcaacga
      121 cgcgatcggc cggagcgctg ccgagtcgcg ctgggtcgat gtcgatcgaa cgtccctggg
      181 cggcgccgga ctccccccag ccggcgccgc ctctccctcg ttccccgtcc tgtcggaggt
      241 cccgtcgtga gactcggtct gctgaccgca ccattcccga acacgcccct tgctgacgtc
      301 gtcgactgga cggccgggca cggcttcgag tcgatcgaga tcgcctgctg gccgccggcg
      361 accggcccca cccgccgcta tgccgggacc tcgcacatcg acgtggcgaa cctgtcgcag
      421 ggcgaggcca ccgacctcgc cgccgggatc cgcggctccg gcctcgtgat ctccgggctc
      481 gggttctatc cgaaccccct ccaccccgat ccggaccatc gggccacggt catcgcccac
      541 ctgaagcacg tgatcacggc cgccgagaag atggacgtcc cgctcgtgaa cacgttcatg
      601 ggcgccgacg ccgcgaagac gcaggaccag aactgggagg acgcactccg gatctggccg
      661 gacatcgtgt cgttcgcggc ggaccacggc cggaagatca cgatcgagaa ctgcccgatg
      721 ctcttcagca acgacgagtg gccgggcggc cacaacctcg cgacgacgcc gcggatctgg
      781 cgccggatca tcgagcagtg gggcggcacc atcgggctga acttcgaccc gagccacctg
      841 atcctccaga tgatcgacat cccgcgcttc atccgtgagt tcgggccgca catcctccac
      901 gtccaggcaa aggacctctg gatcgaccgc gaggggctgt acgagcgcgg cgtgttctcg
      961 atgggcatgg gctggcaggt cccccgcctc cccggcctcg gcgacgtcga ttgggcaccc
     1021 ctcttcggcg ccctctggcg tgccggctac gacggcgacg tgatcatcga acacgaggac
     1081 agggacttcg agaagaccga cgaactggtc aagcgcggat tcctcctggc gcgggacgtc
     1141 ctccgcccct acatcaagtg agcaaggaac acgtgccctt caccgtcgac aacctcaccg
     1201 aacgcgacct cgccaaggcg atcgaccact ccctgctgcg tcctgagctc gatgacgcgt
     1261 tcgtcgaggc gggctgccgc ctcgccgccg agtacgacgt cgcctcggtc tgcgtccgtc
     1321 cggtggacgt ccaccgggcg gtcgccatcc tcgacgggac cgacgtcaag gtcggcaccg
     1381 tcatcggctt cccgcacggc aactcgacaa cggcgacgaa ggtctacgaa gcgcatcggg
     1441 cgctcgagga cggggcggtc gagctcgaca tggtgatcga catcggcgcc ctgaagtccg
     1501 gtcgcgaccg cgacgtccag gccgacatcg ccgccgtcgt cgaggtcgcc cacgcgggcg
     1561 gcgcgatcct caaggtgatc ttcgagaacc attacctgac tcccgacgag atcgtccgcg
     1621 cctgccgtct ggccgaggcg gcgggcgccg agttcgtcaa gacctcgacc ggcttcgcgg
     1681 cgtcgggcgc gacccacgag gacctcgcgc tgatgcgcca gagcgtgtcg gaccacatcc
     1741 aggtcaaggc ggccggcggc gtccggacgc tcgatgccct gatcgacgtg atgaacctcg
     1801 gggtgacgcg gatcggcgca accgccacgg ccgccatcat cgatgacttc cgggcccgca
     1861 aggccggcgc cggcccgctc gccgccaccg ccaccgcagg cgaggccggc tactgatggg
     1921 cgaggtccgg atcgggatgc tcggctcggg gttcatcggc gtcttccacg ccgatgggct
     1981 gcgctacatc cgcgacaccc gggtggtcgc caactggggc gccggcccgg agcggcgcga
     2041 ggcgtttgcc gcccgtttcg gcagccggcc gctcgcgacg atggaggcgg tctgcgcgga
     2101 tcccgaggtc gacctcgtcg tcgtgtcgct gccgaaccat ctccatctcg acgcggtccg
     2161 cgccgcggcg gcgaacggca agggcgtcgc ctgcaccaag ccgctcggac gcaatggcga
     2221 cgaggcggcc gagatgctcc ggctcgtcac cgaggccggc gtgttccacg cgtacctcga
     2281 gaacgtcgtc ttcaacgccg agatgatccg gatgcgcgag atcgtcgagt ccggggcgat
     2341 cggccggctg acgaccttcc gcgccaggga gggtcacagc ggcccgcacg ccgcgcattt
     2401 ctgggacgcc gacctggccg gtggcggcgc gctcctcgac atggcgtcgc atggtgccga
     2461 ggctgcccgc gcgatgttcg gcaaggacct cgcggtgcgc gatgtgttcg cctggggcga
     2521 cacactcgtc cacggcggtc ggacgacgct cgaggacaac gcggtcatgc tcatccggtt
     2581 cgaggacgga cgggtcgcga cgatggatgt ctcgtggtcg tcgaagggcg ggctcgagag
     2641 ccgcttcgag gcctacggcg acggcggccg gatcgtccag gacatcatct cgagcccgct
     2701 ccgcgcgtac atcgaacggc cggccggcta tctcggcgag aaggtcgacg ccgacacggg
     2761 ctgggtctac ccggtcccga acgagaccta cgtccacggc cacgacgcga tgatggccga
     2821 cgtcgtgacc gcgttccgcg agggccgcgc cccgaccgag acgttcgagg acggcctgat
     2881 cgtcaacacg atcctcgacg ccgcctaccg ctcgatcggg agcggccact gggagtcggc
     2941 ccgggtcccg tccgccgcct cgacccgatg acgacgacgt cggttcccgg gctgccctgg
     3001 atgacggcgg ccgccgccct cctcgagctg gtcgccacga cccaggcggc cgcgatcgag
     3061 accgccagcc agtggtcggc tgaggcgatc gccgccgacg gcctcgtcca cctcttcggc
     3121 accggccact cgcggatccc ggtcgaggag atgttcccgc gctacggctc gtacccgggc
     3181 ttcaacccga tcgtcgagct gtcgatgacc ttccacaccc aggtcgtcgg cgcgaacggc
     3241 cagcgccagg cgatgttcat cgagcggaca cccggtctcg ccgaggtgat cctgtcgaac
     3301 ttctc
//
LOCUS       DATAIW010000362         5835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_189016, whole genome shotgun sequence.
ACCESSION   DATAIW010000362 DATAIW010000000
VERSION     DATAIW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189016"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VHM48_10900"
     CDS             <1..300
                     /locus_tag="VHM48_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2755965.1"
                     /translation="HNDLVLDPERHEVTIAGSMVQLTHVEFRLLQSLLEADGRVLSRD
                     QLLDAVWGAEQSEVLDRTVDVHIRRLRDKLGDDPDAPRYVATVRGAGYRSAPVAG"
     gene            327..1412
                     /locus_tag="VHM48_10905"
     CDS             327..1412
                     /locus_tag="VHM48_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2755966.1"
                     /translation="MRSRGIATRIALAALASAAIGLVILALGVTVVGGQVFTDLMMKA
                     GDSAEHARTMYDQSVTTVVIAAVLVAGLASVGLAIVMGRMLARPLRAVGVAARRIADG
                     DYAARVPRDGPEEIASLADSFNQMAASLEEQERMRRDFIANAAHELRTPLTNLQGYLE
                     ALRDGVIVADRSTYESLWDEAERLVRLSRSLDSLAEGDAATSPPDLQSIDLAAAVRSA
                     VELAQPAIERAGLRLSLDVPASLPARANPDHLAQVLANLLSNAVRYTPAGGLVAVRAA
                     RRPGDLLVSVTNSGEPIPPDDLARVFERFYRVEKSRDQARGGAGIGLAIVKQLVEAGG
                     GRVGAESTDGKTSFWFSLPAQPVVRSS"
     gene            complement(1542..2444)
                     /locus_tag="VHM48_10910"
     CDS             complement(1542..2444)
                     /locus_tag="VHM48_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2755967.1"
                     /translation="MSIGLELGLIAAVCWGSTDVSAAIAGRRLGSLGVTAIVQATSLA
                     AILALCLVRGSWFPTKIEDVVVAVVTGVVAAGAYVSFFTALRIGPLSVVSPVVAAYGG
                     LTVILAVLVRGETLTSLQAVGAALATLGVILTGLVSDGGWRNTKVAGKGVGFAIVALV
                     CFAILTIGLAGPIRSAGWLPVLLTARIANAATVALVMLVVMVFRPRAAAPLLAQATDR
                     PPGRLPTAVLAAIAGGALDIAGFVAFAFGLEQAPAWILGLASSFGPVVSVVVAVVLWG
                     ERLQPSQWIGLAGIGLGLVAVALP"
     gene            2527..2602
                     /locus_tag="VHM48_10915"
     tRNA            2527..2602
                     /locus_tag="VHM48_10915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2560..2562,aa:Ala,seq:cgc)
     gene            2691..3218
                     /locus_tag="VHM48_10920"
     CDS             2691..3218
                     /locus_tag="VHM48_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX2755968.1"
                     /translation="MKGRSPRILLIGMMGSGKTTIGRELGRRTGWPFLDNDALVRELT
                     GREPAAIDADDGEDALHAAEARAFQMATDRSGPAIVAVAGWIVDDDAARARLAAAGHV
                     VWLRARPDSLRTRIGSGAGRRADAQDVGWIEARATEREPRYRETADQVVDVDDLDPAA
                     IAAAILDAIGLPSAG"
     gene            complement(3207..3989)
                     /locus_tag="VHM48_10925"
     CDS             complement(3207..3989)
                     /locus_tag="VHM48_10925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX2755969.1"
                     /translation="MAPPYLLRRSPRSRRLRITIDPEHGLVVTVPLATRRGWAHPERM
                     IDDFLRDREPWIRRHLLQQARQRAELAARGGLADGASIRYLGELHRLRVASSGTGRRS
                     TVARLVTLAGPELHVGLLPRDVERVDRVLEAWFKDQARFAIDGTIADHAGALEVSPAA
                     IAIRDPRSRWGSASRQGRLMFSWRLVLAPPEALETVVVHELAHLKVFGHGPRFWAVVA
                     SRRPDHLVWRRWLRSHSLELHGALTASTTAGVDPVALESSAG"
     gene            complement(4018..>5835)
                     /locus_tag="VHM48_10930"
     CDS             complement(4018..>5835)
                     /locus_tag="VHM48_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2755970.1"
                     /translation="RVERAQQALERKNGSEVELSKSGEIVPVMAPPVLPPQPRTPARE
                     ALLLEIKHHLVTEVIAAFDTLLDVNATDVRTKMEGIVDRTIAVHGFAVTRDERLRLVD
                     ELADDVTGFGPLEPLLHDETITEVMVNGPNHVYIERAGKIERVDSSFLNDEHVLRIID
                     RIITPMGRRIDEMSPRVDARLPDGSRVNAIVEPLSLVGPVITVRKFSSKPFTVDDLIR
                     FGTATAEMFEFLRVCIEARLNLFVSGGTGSGKTTTLNVLSSFIPNDERIVTIEDAAEL
                     QLRQDHVITLESRPPNLEGEGEITIRHLLRNAMHMRADRLIVGECRAGEALDMLQAMT
                     TGHDGSLSTGHANTPKDMLRRLETMVLMTGYELPLRAIREQMASAVDLIVHTSRLKDG
                     TRKIVNITEVYGIEDDEILTQDIFAFEQTGFEDGKIQGELKPTGVRPTFMDQFHKAGI
                     KLPPGEYGIPPEDLAKPSRISSTKGRWGAGDSSAGPIAPTLPLGYGKAVKAGGMVYVS
                     AMGPIDPDTGQVVGSVITAQSRQCLANLKAKLEAAGSSLDKVVWANWSLRDPTEFDLF
                     NEEWVRWFPGDGPVGQSTQMTPLQRRAGFRVSIGVIAEA"
BASE COUNT          878 a         2053 c         2082 g          822 t
ORIGIN      
        1 cacaacgacc tcgtcctcga ccccgagcgc cacgaggtca cgatcgccgg gtcgatggtc
       61 cagctgaccc atgtcgagtt ccgcctgctc cagtccctcc tcgaggcgga cgggcgggtc
      121 ctcagccgcg accagctcct cgacgccgtg tggggcgccg agcagtccga agtcctcgat
      181 cggaccgtcg atgtccacat ccgccgcctg cgcgacaagc tcggcgacga cccggatgcg
      241 ccgcgttacg tcgcgaccgt ccgcggcgcc ggctaccgct ccgcgccggt ggcgggctga
      301 gcccggtcgt ggcgacggcc acgaggatgc gcagccgagg gatcgccacc cggatcgctc
      361 tcgccgcgct ggcctcggcc gcgatcgggc tcgtgatcct ggccctcggg gtgaccgtcg
      421 tcggcggcca ggtgttcacc gacctgatga tgaaagctgg cgactcggcc gagcacgccc
      481 ggacgatgta cgaccagtcg gtcacgaccg tcgtgatcgc cgcggtcctg gtcgcgggcc
      541 tcgccagcgt cggactggcg atcgtcatgg gccgaatgct ggcgcggccg ctgcgcgcgg
      601 tcggggtcgc cgcccgccgg atcgccgacg gcgattatgc cgcccgggtc ccgcgcgacg
      661 ggccggaaga gatcgcgagc ctcgccgatt ccttcaacca gatggccgcc agcctcgagg
      721 agcaggagcg gatgcggcgc gacttcatcg ccaacgctgc ccacgagctg cggaccccgc
      781 tgacgaacct gcagggctat ctcgaggcgc tgcgcgacgg cgtgatcgtc gccgaccgct
      841 cgacgtacga atcgctctgg gacgaggccg agcggctggt ccggctgtca cgatcgctcg
      901 attccctcgc cgagggcgat gcggcgacga gcccgccgga cctccagtcg atcgacctcg
      961 cggccgctgt ccggtcggcg gtcgagctcg cccagccggc gatcgagcgg gcgggcctgc
     1021 ggctctcgct cgacgtcccc gccagcctgc cggcccgggc caaccccgac cacctcgccc
     1081 aggtcctcgc caacctgctc tcgaacgcgg tccgctacac gccggccggc gggctggtcg
     1141 cggtccgcgc cgcgcgtcga cccggcgatc tgctggtctc ggtcacgaac agtggcgagc
     1201 cgatcccgcc tgacgatctc gcccgggtct tcgagcgttt ctaccgggtc gagaagtcgc
     1261 gcgaccaggc ccgcggcggg gccggcatcg gcctcgcgat cgtcaagcag ctcgtcgaag
     1321 ccggcggcgg ccgggtcggg gccgagtcga ccgacggcaa gacgagcttc tggttcagcc
     1381 tcccggcgca gcctgtcgtc cgatcgtcgt gagccccccg aaactccttt cgagcgtcaa
     1441 cgagcgcggg gccgacgcgc atcgcgcgat ttcgtgcccg gccgtgcgtg cagatcgccg
     1501 acttgagtcc gagcagactc agaccgagac gactcggagt ctcacggaag cgcgaccgcc
     1561 actaacccga gaccgatccc ggcaaggccg atccactggc tcggctggag gcgttcgccc
     1621 cacaggacga ccgccacgac cacgctcacg accggcccga acgagctggc caggccgagg
     1681 atccaggcgg gcgcctgctc gagcccgaac gcgaacgcca cgaagcccgc gatgtcgagc
     1741 gcaccgccgg cgatggccgc gaggactgcg gtcggcagtc gcccgggagg tcgatcggtc
     1801 gcctgggcga ggagcggcgc ggccgctcgc ggtcggaaca ccatgacgac gagcatgacg
     1861 agcgcgacgg tcgccgcgtt ggcgatccgg gcggtcagga ggaccggcag ccagccggcg
     1921 ctccggatcg ggccggccag gccgatcgtc aggatcgcga aacagaccag ggcgacgatc
     1981 gcgaacccga cgcccttgcc ggcgaccttc gtgttccgcc aaccgccatc cgacaccagg
     2041 ccggtcagga tgacaccgag cgtcgcgagc gcggcgccga ccgcctggag cgacgtcagc
     2101 gtctcgcctc gaacgaggac ggcgaggatg accgtcaggc cgccgtacgc agcgaccacc
     2161 ggactcacga ccgacaacgg gccgatgcgc agggccgtga agaacgacac gtaggcccct
     2221 gccgcaacga cgcccgtcac gaccgcgacg acgacgtcct cgatcttcgt cgggaaccac
     2281 gagccgcgga cgaggcacag ggcgaggatc gcggcgaggc tcgtcgcctg gacgatcgcg
     2341 gtgacgccga ggctcccgag acgccgcccg gcgatcgccg ccgagacgtc ggtcgagccc
     2401 cagcagaccg ccgcgatcag gccgagctcg agaccgatgg acaccgccgg agggtagtcg
     2461 gacggggggt ccgagagagg ccccgtgcta cacttcggga ccgtttgcgc ctccgcgcga
     2521 cgccgtgggg atgtagctca gctggcagag caccgcgttc gcatcgcggg ggtcaggggt
     2581 tcgagtcccc tcatctccac caccttcctc accctgaccc agccctgatg cgcgcgtccg
     2641 acgcgggacc gacccgaccg gcgtgccgct ggtcacgcca tgatggccgc atgaagggcc
     2701 ggtccccgcg gatcctcctg atcggcatga tgggttcggg caagacgacg atcgggcgcg
     2761 agcttggccg acggaccggc tggccgttcc tcgacaacga cgctctcgtc cgcgagctga
     2821 ctggacgcga gccggcggcg atcgatgcgg acgacggcga ggacgccctc cacgccgcgg
     2881 aggcacgggc gttccagatg gccaccgacc gctcgggccc ggcgatcgtc gcggtggcgg
     2941 gctggatcgt cgacgacgac gccgcgcgag cccgactcgc cgcggccggg cacgtcgtct
     3001 ggctccgggc gcgacccgat agcctgcgga cacggatcgg atcgggcgcg ggtcggcgtg
     3061 cggatgcgca ggacgtcggg tggatcgagg cgcgggcgac cgagcgcgag ccacgctatc
     3121 gcgagaccgc cgaccaggtg gtcgatgtcg acgacctcga tcccgcggcc atcgccgccg
     3181 cgatcctcga cgcgatcggg ctgccgtcag ccggctgacg actcgagcgc gaccggatcg
     3241 acaccggcgg tcgtcgacgc ggtcagggcg ccgtgcagct cgagcgaatg cgaccgcagc
     3301 cagcgccgcc agacgaggtg gtccggtcgg cgcgaggcca cgaccgccca gaaccgcggc
     3361 ccgtggccga acaccttcag gtgggcgagc tcgtggacga cgacggtctc gagcgcctcc
     3421 gggggagcca gcaccagcct ccagctgaac atcagtcggc cctgccgcga ggcgctcccc
     3481 catcggctgc gcgggtcacg gatggcgatc gcggccgggc tgacctcgag cgcgccggca
     3541 tggtcggcga tcgtcccgtc gatcgcgaac ctggcctgat ccttgaacca cgcttcgagg
     3601 acccggtcca cccgttcgac atcacgcggg agcagcccga cgtggagctc cggaccggca
     3661 agggtcacca accgggcaac ggtcgatcga cggcctgtgc cggacgacgc gacccgcagt
     3721 cgatggagct cgccgaggta ccggatcgac gctccgtcag ccaggccgcc gcgggccgcg
     3781 agctcggctc gctggcgcgc ctgttgcagg agatgacgcc ggatccacgg ctcccgatcg
     3841 cgcaggaagt cgtcgatcat ccgctccggg tgagcccagc cgcggcgcgt cgccagggga
     3901 accgtgacga cgagaccgtg ctcggggtcg atcgtgatcc ggagccggcg cgaccgagga
     3961 gagcggcgga ggagatacgg cggcgccatc gaagccgatg gtgccacacg gggctggcta
     4021 cgcctcggcg atcaccccga tcgagacacg gaagcccgcc cggcgctgga gcggcgtcat
     4081 ctgggtcgat tgaccgaccg gaccgtcacc cgggaaccag cgcacccatt cctcgttgaa
     4141 caggtcgaac tcggtcgggt cgcgcaacga ccagttggcc cagacgacct tgtcgagcga
     4201 gctcccggcc gcctcgagct tggccttgag gttcgccagg cattggcgtg actgggccgt
     4261 gatgacgctg ccgacgacct ggccggtgtc gggatcgatc ggtcccatcg ccgatacgta
     4321 gaccatcccg ccggctttga ccgccttgcc gtagccgagg ggcagggtcg gggcgatcgg
     4381 ccccgcagac gaatcgccgg cgccccagcg acccttcgtc gagctgatcc ggctcggctt
     4441 ggcgaggtcc tcgggcggga tcccgtattc gccgggcggg agcttgatgc cggccttgtg
     4501 gaactggtcc atgaaggtcg gacggacgcc ggtcggcttc agctcgccct ggatcttgcc
     4561 gtcctcgaag ccggtctgct cgaacgcgaa gatgtcctgg gtcaggatct cgtcgtcctc
     4621 gatgccgtag acctcggtga tgttgacgat cttgcgcgtg ccgtccttga gccgggacgt
     4681 gtggacgatc aggtcgaccg cggacgccat ctgctcgcgg atcgcccgga gcggcagctc
     4741 gtagccggtc atcaggacca tcgtctcgag ccgtcggagc atgtccttcg gggtgttggc
     4801 gtggccggtc gagagcgagc cgtcgtggcc ggtcgtcatc gcctggagca tgtccagcgc
     4861 ctcgccggca cggcactccc cgacgatgag ccggtcagca cgcatgtgca tcgcattccg
     4921 gagcagatgg cggatcgtga tctcgccctc gccctcgagg ttcggcgggc gcgattcgag
     4981 ggtgatgacg tggtcctggc ggagctgcag ctcggccgcg tcctcgatgg tgacgatgcg
     5041 ctcgtcgttg gggatgaagg acgagagcac gttgagggtc gtcgtcttgc cggagccggt
     5101 gccgcccgag acgaagaggt tgaggcgggc ctcgatgcag acccgcagga actcgaacat
     5161 ctcggccgtg gctgtgccga agcggatgag gtcgtcgacg gtgaacggct tcgaggagaa
     5221 cttgcggacc gtgatcaccg ggccgacgag cgacagcggt tcgacgatcg cattgacgcg
     5281 cgagccgtcc ggcaggcggg cgtcgacccg cgggctcatc tcgtcgatgc ggcggcccat
     5341 cggggtgatg atccggtcga tgatccggag gacgtgctcg tcgttgagga acgagctgtc
     5401 gacccgttcg atcttcccgg cccgctcgat gtagacgtgg ttcgggccgt tgaccatgac
     5461 ctcggtgatc gtctcgtcat ggaggagcgg ctcgagcggc ccgaagccgg tgacgtcgtc
     5521 ggccagctcg tcgacgaggc ggagccgttc atcgcgggtc accgcgaacc cgtggaccgc
     5581 gatcgtccgg tcgacgatcc cctccatctt ggtgcggacg tcggtcgcgt tgacgtcgag
     5641 gagcgtgtcg aacgcggcga tcacctcggt caccaggtga tgcttgatct cgagcagcag
     5701 cgcctcccgg gcgggggtcc gcggctgggg cggcagcacc ggaggagcca tgaccggcac
     5761 gatctcgccg gacttggaca gctcgacctc gctgccgttc ttgcgttcga gtgcctgctg
     5821 cgcccgttcc acccg
//
LOCUS       DATAIW010000363         9872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_189086, whole genome shotgun sequence.
ACCESSION   DATAIW010000363 DATAIW010000000
VERSION     DATAIW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189086"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VHM48_10935"
     CDS             complement(<1..521)
                     /locus_tag="VHM48_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2755971.1"
                     /translation="MLSGRPGGQGAPRILIVDDDPNLLVLLADQLRADGFDIQTARDG
                     DEALRRLKSGWPDLLIIDMMMPRMDGLTLAREIKGRADLPIIVLSAIDAGDSKADLLE
                     EVAEDYVTKPYHYPELRARIHRILRRLGDKVPRQSLALGANLTLDLHRREATVAGDTV
                     QLTPTESRLLYALA"
     gene            complement(768..1577)
                     /locus_tag="VHM48_10940"
     CDS             complement(768..1577)
                     /locus_tag="VHM48_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755972.1"
                     /translation="MTDRQLLLRVGAIVKDLRLAIRWSQRELAARSGVSQSLISAIEN
                     GRVRAMTFVTIQTLVETMGGRLIVDVARPFLGDRPLQGDAAHARCVSFVAGRLRRAGW
                     QVATEIEIGGDRSRGWIDVLAFDPRTGVVLVIEVKTEIHDVGGIERALGWYEREAWTA
                     ARRRGWQPRSTVGFLVLLATAANDERLQENRSAFAAGFPTRARSLMELVERAPQSGRV
                     GRGLAMVDPLSRRRAWLRPTVTDGRRSPAPHADYVSFLAKIQRRPRGRAVG"
     gene            complement(1780..2634)
                     /locus_tag="VHM48_10945"
     CDS             complement(1780..2634)
                     /locus_tag="VHM48_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755973.1"
                     /translation="MSPTRPAPKRATKPSPKPKRKRARPDPAIAWAKRIARYRPRLLE
                     DTLDSLGELYGPQIWRRRLDPTSELILTILTQNSADINAEKAFIALRAAWPSGRAEEI
                     HEPGAGWGGGGLPPGAPPDWIAVENAPLPQLVDVIRPGGLAPQKAPRIQATLRKIREE
                     RGDHSLEFLGDLAPLAARDWLTSIPGIGKKTASVLLLFCFNQPLMPVDRHVERVAKRI
                     GLLPARAPADLAHEQFIAMLAGAPERSYETHVLLIHHGREICQARSPKHDLCPIRGRC
                     RMVDPKAP"
     gene            complement(2631..3791)
                     /locus_tag="VHM48_10950"
     CDS             complement(2631..3791)
                     /locus_tag="VHM48_10950"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX2755974.1"
                     /translation="MTDAAGRSFVILIAPDSFKGSLTSVEVARALAAGWARARPRDEI
                     LLAPLADGGEGTTVAIEAAGGWTWRVADATDPIGRAISGRWLQSVDGGRAIVEMAAAS
                     GLSRLAGSELDPLGATTRGTGELLVAALDAGVRDITLGIGGSATNDGGAGILRALGAQ
                     VSDAAVDPTDVDLAGLDPRLAETRLRIACDVSNPLLGERGAAATYGPQKGATPDDVAE
                     LDRRLGRSADALEEAAGRRERDTAGAGAAGGTGFGLLCLADRFASLELVPGIDVVMAA
                     ADFDGKLARADLVITGEGRIDAQTAFGKTALGVARRASEARVACIAVGGGVEPDGIEA
                     LRPLGAVVVPVTERPQSVAEAMAAGPAPLERCGERLARVIDLGRRLGASSGS"
     gene            complement(3788..5770)
                     /locus_tag="VHM48_10955"
     CDS             complement(3788..5770)
                     /locus_tag="VHM48_10955"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX2755975.1"
                     /translation="MNAAARGPLPARILIPVANPATAEELIRLGAALLDPRAGELTAL
                     GIVEVPEGMPLSEGATRARHARRLLQRVLDFAPDGTTIHPIVRIGRHAAEGIIEAAAE
                     QEADLIIFGWGGKAPAGREGGSVISPTIDEVVRDAPADIAVVKQRGSKEIRRIVVPVR
                     GGPHAELAVRYADAIARRTDAIVSVLHFVPPGITLAVRAQAEHALAQFVRQHLKGRGE
                     GIVREATNVRNAILKEAERADLVVMGASAQPAGTDGDTYLFGALPEAIATRAKPTVIV
                     VKTRELIGRATFEQLASRAETLAAADRAAEEARAVPVRVERWFGESNFHHAEFADLRR
                     LVQLKEKQGVTVSLVLPTLNEEETIGPIVRRAMREMMGRVPLIDEILVIDSVSTDRTR
                     EIAEAEGARVVQHPDILSRYGSFIGKGEALWKSVHETTGDIIVWADTDVRNWHPRMVY
                     GTLGPLLHEPRLQYVKGYYQRPIVEGGVLKEGGGGRVTELVARPLINLFFPELSGMIQ
                     PLSGEYAGRRALLESIPFFTGYAVEIGHLIDATERVGIEGLGQVDLERRVHRNQELEG
                     LSRMSFVILQAVMKRLEERRKARLFAELGSTMKLPRSGRGRLSLEVIELADQERPPMI
                     RIPEYLERRRGVEGSDGASGHGRAGALSDELEPVRR"
     gene            complement(5767..7296)
                     /locus_tag="VHM48_10960"
     CDS             complement(5767..7296)
                     /locus_tag="VHM48_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755976.1"
                     /translation="MPAQGLTPAVRDEIARLGHADIMVGIPSFKNAATIGYVVRAAQA
                     GLVQYFPDLRPVLVNSDAGSPDGTSRVVIDTEPPDYVEQILLVRPTNKLSRVSLTYPE
                     IDGVGGKGAALRTIFEIAAALEVQALVVVDSDLRSIIPEWIELLAGPILKGGYDYVAP
                     LYARYKYDGTITNTVTYPLTRALYGNRIRQPIGGDFGVSGDLVKHYLTLDDWTEDISK
                     FGIDIWMTTSALTGGFAVCQARLGAKVHDPKDPGADLGPMFRQVVGTILRLAATHPDK
                     WLAIRGSHDIPAYGFERIIDPPPLEVNTLRLLTEFHTGSLTLADTWRRMFAPPNLEMV
                     LVLAHEAGKLVDAARARLGIAGDGSSTNATTLEMADALSAFQFSDDLWARLIYDLVIC
                     ARSAEPPIENLVAALVPIYFGRVGSFVIENRHQTTDEAEDRVERQARAFEHLKPYLVE
                     RWEASVAADDTAAATAMAAPTAIAASNGASPAGAQTATPPASAAKPARRASAGPSDPI
                     R"
     gene            complement(7615..8313)
                     /locus_tag="VHM48_10965"
     CDS             complement(7615..8313)
                     /locus_tag="VHM48_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755977.1"
                     /translation="MIATDAASAAATWRPQLFGGKRANSIVDPIIEPLWSGPRILAFV
                     DGPTIQLTDIEGGLVDSKPDIIDALREAVAGGTALVEAYLTPEPFQDQALIAARDPVT
                     LPKPGEAMTRMLVGTRGDRKDRLVKRQEEARQWTVDSTTDRLALVAVDLLWLDDESLC
                     DVPQLERKRILESVLPESDLIRVGIHVKPPIDAWLGSWRSFGFRRLAYKGANSRYVPG
                     QKNPEWALAQIPAR"
     gene            8444..9811
                     /locus_tag="VHM48_10970"
     CDS             8444..9811
                     /locus_tag="VHM48_10970"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755978.1"
                     /translation="MAASKDVPVARSGRGLGALSPSGRAAEPFAWSLYDFANTIFSYA
                     VVSTAIGLWATDGSRFGERDGNLVVSIAVAISVALNAIVSPILGALSDRGGRRLPFLL
                     FFTVLCIVPTALIGPSPAIVGIALFAVANFGYQAALIYYDATLKTVSWPETRGRLSGI
                     GVAVGYCGTIFVGLVLILLKVPIEGRFLVAAILFAVFSVPIFVVVKEQGGVGRISRSD
                     VTGSWSQLRTTIDHARAVPGLGRFLLGRFFYSDAVNTIIVVMTVVTVYAKGLSESTAQ
                     IVLLGLTVVAVLASFGWGRLVDAWGPKRTLMVILASWAVGLVLGAVSLGVDGPIGFVA
                     FLVAGAILGSGLGGVQVADRVFMVRLSPPERLGEFFGLYGLVGKGSQVIGQMLYGLTL
                     FLLLDTFKVGAYQLAVLTLLVTMAIGYRLIQPVSDTWAGAHETDGTPAPPERLAPSAA
                     PIEPR"
BASE COUNT         1353 a         3422 c         3570 g         1527 t
ORIGIN      
        1 gcaagcgcat agaggaggcg cgactcggtc ggcgtcagct ggacggtgtc gccggcgacg
       61 gttgcctcgc gccgatggag atccagggtc aggttggccc cgagggccag gctctgccgt
      121 gggaccttgt cgccgagccg ccgcaggatc cggtggatcc gggctcgcag ctccgggtag
      181 tggtagggct tggtgacgta gtcctccgcg acctcctcga ggaggtcggc cttggagtcg
      241 ccggcatcga tcgccgacag gacgatgatc ggcaggtcgg cccgaccctt gatctcgcgg
      301 gccagggtca ggccgtccat ccgcggcatc atcatgtcga tgatgaggag atccggccag
      361 ccgctcttga ggcggcggag ggcctcgtcg ccatcgcgcg ccgtctggat gtcgaagccg
      421 tcggcgcgga gctggtcggc gagcaggacc aggagattcg ggtcgtcgtc gacgatcagg
      481 atccgcggcg ccccctgtcc gcccggacgt ccgctcagca tcgccggctc agcatggccg
      541 gagcatagcc gacgcccgct gccgcgcgaa ctgcgcgccc gcgcgcggtc cccaccccgc
      601 cccgcgcgaa ctgcgcgccc gcgcgcggtc cgcaccccgg aggctgatca gtgtggttga
      661 tcgattcacg gcccggtgag cgtgacggat acctcagatc ggcgatctgg cgctggggtg
      721 ggcgagccgc cgtgcgcggc cgccgcggaa tcgatcagcg agaacgatca gccaactgcg
      781 cggcctcggg gtcgccgctg gatcttcgcc aggaacgaca cgtaatcggc gtgcggagct
      841 ggcgatcgcc ggccgtccgt cacggtgggc cgcaaccatg cccgacgtcg gctcagcggg
      901 tcgaccatcg cgaggccgcg acctactcgc ccggactgcg gcgctcgttc gacaagctcc
      961 atgagggacc gcgcccgcgt cggaaacccc gccgcgaacg cggaccgatt ctcctggagc
     1021 cgctcgtcat tggcggccgt ggcgaggagc accaggaatc cgacggtaga tcgcggctgc
     1081 caccctcgac gacgcgcggc tgtccatgcc tcccgctcat accagccgag cgcgcgctca
     1141 atgcctccga cgtcgtggat ctcggtcttg acctcgatga cgaggacgac tccggttcgg
     1201 gggtcgaacg cgagcacgtc gatccagcca cgggaccggt cgccgccgat ctcgatctcg
     1261 gtcgcgacct gccagccggc gcgccggagt cgaccggcta cgaacgagac gcatcgagcg
     1321 tgggccgcgt cgccctgaag cggccgatcg ccgaggaacg gccgtgcaac gtcgacgatg
     1381 agacggccgc ccatggtctc gacgagcgtc tggatggtca cgaatgtcat ggcgcgaacc
     1441 cggccattct cgatggccga gatcagggac tgtgacacgc ccgatctggc cgccagctcg
     1501 cgctggctcc accggatcgc cagtcgcagg tccttgacga tcgcgccgac ccgcaggaga
     1561 agttgtctgt cggtcatggc ggaggttgta gaggacggcc cttatccggc gattgactgc
     1621 gctccgattg ctgccactga tcgatctcgt gacccgcgag cgcggcgcat gccgcgttcc
     1681 cgcgtcgagg tgtctgagca ggcgcgcggt cacgctcgct gagccggaaa ccgatcggtg
     1741 aacgcgatca cgcggcgcgg cacggcgcga cgacccggat cagggggcct tcgggtcgac
     1801 catccggcag cggccgcgga tcgggcagag gtcgtgcttt gggctgcggg cctggcagat
     1861 ctcgcggccg tgatggatca gcaggacgtg cgtctcgtac gatcgctcgg gggcgccggc
     1921 gagcattgcg atgaactgct catgggcaag gtccgccggc gccctggccg gcagcagccc
     1981 gatccgcttg gcgacccgct cgacgtggcg gtcgaccggc atcaggggct ggttgaagca
     2041 gaacaggagg aggaccgacg cagtcttctt gccgatgccc gggatcgatg tcagccaatc
     2101 gcgggcggcc agcggcgcca gatcgccgag gaactccagc gagtggtcac cacgctcctc
     2161 gcggattttg cggagcgtgg cctggatgcg cggcgccttc tgcggggcga gcccgcccgg
     2221 ccggatgacg tccaccagct gcggcaacgg cgcgttctcg accgcgatcc agtccggtgg
     2281 cgcgccgggt ggcaacccgc ccccacccca gccggccccc ggttcatgga tctcctcggc
     2341 acggcctgat ggccaggccg cccggagcgc gatgaaggcc ttctcggcgt tgatgtcggc
     2401 gctgttctgg gtgagaatcg tgaggatcag ctccgaggtc gggtcgaggc gccgccgcca
     2461 gatctgcggc ccgtagagct cgccgagcga atcgagggtg tcctccagca gccgcggccg
     2521 atagcgcgcg atgcgcttcg cccacgcgat cgcgggatcg ggcctggctc gtttccgctt
     2581 gggcttcgga gacggcttgg tcgcgcgttt cggcgcgggt cgcgtcggac tcatgaaccc
     2641 gatgatgcgc caagacggcg cccgaggtcg atcacccgcg caagccgctc gccgcagcgt
     2701 tcgaggggcg ccgggccggc ggccatcgcc tccgcgacgc tctgcggccg ctcggtgacc
     2761 ggtaccacga ccgcaccgag gggtcgcagt gcctcgatcc catcgggttc gactccgccg
     2821 ccgacggcga tgcaggcaac cctggcttcg gaggcacggc gcgccacccc gagggccgtc
     2881 ttgccaaacg cggtctgggc gtcgatccgg ccctcgcccg tgatcacgag gtcggcccgg
     2941 gcgagcttcc cgtcgaaatc ggcggcggcc atgaccacat cgatgccggg aaccagctcg
     3001 agggaggcga accgatcggc aaggcagagg aggccgaacc ccgtcccgcc ggcggcgcct
     3061 gcacccgccg tgtcgcgctc ccgccggccg gccgcctcct cgagcgcgtc cgcggaccgg
     3121 ccgagtcgcc ggtcgagctc cgcgacgtcg tcgggcgtgg cgcccttctg cggtccgtag
     3181 gtcgcggcgg cgccgcgctc tccgagcagc gggttcgaaa catcgcaggc gatccgaagt
     3241 cgcgtctcgg caaggcgcgg atcgagcccg gccagatcca cgtcggtcgg gtcgaccgcg
     3301 gcgtccgaaa cctgggcgcc gagggcacga aggatcccgg cgccgccgtc gttggtggcg
     3361 cttccgccga tcccgagcgt gatgtcgcgg acgccggcat cgagggctgc gacgagcagc
     3421 tcgcccgtgc cgcgggtcgt cgcgcccagc ggatcaagct ccgagccggc gagacgtgag
     3481 agcccggacg ctgccgccat ctcgacgatc gcccgcccgc cgtcgacgga ctgcagccag
     3541 cgaccggaga tcgctcgtcc gatcgggtcc gtggcatcgg ccacccgcca cgtccagccg
     3601 ccggccgcct cgatggcgac ggtcgtgccc tcgccaccgt ccgcgagcgg cgcgagcagg
     3661 atctcgtcgc gcggccgcgc ccgtgcccag ccggcggcca gtgcccgcgc gacctcgacc
     3721 gaggtcagcg atcccttgaa cgagtccggg gcgatcagga tgacgaagga acgcccggcg
     3781 gcgtcggtca ccgtcggacg ggttcgagct cgtcggagag cgcgcctgcc cgaccgtggc
     3841 ccgaggcgcc gtccgagccc tcgacaccgc gccgccgctc gagatattcc gggatccgga
     3901 tcatcggcgg ccgctcctgg tccgccagct cgatcacctc gagcgacagc cggccacggc
     3961 cggagcgcgg cagcttcatc gtcgagccga gctccgcgaa caggcgcgcc ttgcgtcgtt
     4021 cctcgagccg cttcatcacc gcctggagga tcacgaacga catccgcgac aggccttcga
     4081 gctcctggtt gcggtggacc cgccgctcga ggtcgacctg gccgaggccc tcgatcccga
     4141 cccgctcggt ggcgtcgatg aggtgcccga tctcgaccgc gtaaccggtg aagaagggga
     4201 tcgattcgag gagcgcccgc cgcccggcat attcgccgct gagcggctgg atcatcccgg
     4261 acagctccgg aaagaacagg ttgatcaggg gccgggccac gagctcggtg acgcggcccc
     4321 cgccgccctc cttgaggacc ccgccttcga cgatcgggcg ctggtagtag cccttgacgt
     4381 actgcaggcg cggctcgtgg agcagcggcc cgagcgtccc gtagaccatc cgcggatgcc
     4441 agttgcggac atcggtgtcg gcccagacga tgatgtcgcc ggtggtctcg tggaccgatt
     4501 tccagagcgc ctcgcccttg ccgatgaacg agccgtagcg cgacaggatg tcgggatgct
     4561 ggacgacgcg ggcgccctcg gcctcggcga tctcgcgggt ccgatcggtc gagaccgagt
     4621 cgatcacgag aatctcgtcg atcagcggga cccggcccat catctcgcgc atcgcccgac
     4681 ggacgatcgg cccgatcgtc tcctcctcgt tgagggtcgg caggacgagc gagaccgtga
     4741 cgccctgctt ctccttgagc tggacgagcc ggcggaggtc ggcgaactcg gcgtggtgga
     4801 agttcgactc gccgaaccag cgctcgaccc ggacggggac ggctcgcgcc tcctcggccg
     4861 cgcggtcggc cgcagcgagg gtctcggcac gcgacgcgag ctgctcgaag gtggcccggc
     4921 cgatcaactc gcgggtcttg acgacgatga ccgtcggctt ggcccgcgtc gcgatcgctt
     4981 cgggcagggc tccgaagagg taggtgtcgc cgtcggtgcc ggctggctga gcggacgccc
     5041 ccatgaccac gaggtcggcc cgttcggctt ccttgaggat cgcgttgcgg acgttggtcg
     5101 cctcacggac gatcccctcg ccgcggccct tgaggtgctg ccggacgaac tgggcgaggg
     5161 cgtgctcggc ctgggcgcgg acggcgagcg tgatgccggg tgggacgaaa tggaggacgc
     5221 tgacgatggc gtcggtccgg cgggcgatcg cgtcggcata gcggaccgcg agctcggcat
     5281 gtggcccgcc gcggaccggg acgacgatcc gccggatctc cttgctgccg cgctgcttga
     5341 cgaccgcgat gtcggccggc gcgtcgcgga cgacctcatc gatcgtcggc gagatgaccg
     5401 agccgccctc ccgaccggca ggcgccttgc cgccccagcc gaagatgatc aggtcagcct
     5461 cctgctcggc cgccgcctcg atgatcccct ccgcggcgtg gcggccgatc cggacgatcg
     5521 ggtggatcgt ggtgccgtcc ggggcgaagt cgagaacccg ctggaggagt cgccgggcgt
     5581 ggcgggcccg ggtcgcgcct tcgctgagcg gcatgccctc gggcacctcg acgatgccga
     5641 gggcggtgag ctcgccggcc cgcgggtcga ggagggcggc accgagccgg atcagctcct
     5701 cggccgtggc cgggttggcg accgggatca ggatccgggc gggcagcggg ccacgggcgg
     5761 cggcgttcat cggattgggt cgcttggacc ggccgaggcg cggcgggcgg gcttggcagc
     5821 cgaggccggc ggcgtggccg tctgcgcgcc ggcgggcgat gcgccgttgg acgccgcgat
     5881 ggccgtcgga gcagccatcg ccgtggccgc ggcggtgtcg tcggcggcga cggacgcctc
     5941 ccagcgctcg acgagatacg gcttgaggtg ctcgaacgcg cgcgcctggc gctcgacgcg
     6001 atcctcggcc tcgtcggtcg tctggtggcg gttctcgatc acgaagctcc cgacccgccc
     6061 gaagtagatc gggacgaggg cggcgacgag attctcgatc ggcggctcgg ccgagcgggc
     6121 gcagatcacg aggtcgtaga tcagccgtgc ccacaggtcg tcggagaact ggaatgcgga
     6181 cagcgcatcg gccatctcga gggtcgtcgc gttggtcgac gagccgtcac cggcgatccc
     6241 gagccgcgcc cgggccgcat cgacgagctt gcccgcctcg tgggcgagga ccaggaccat
     6301 ctcgagattc ggtggcgcga acatccgccg ccaggtgtca gcgagggtca gcgagccagt
     6361 gtggaactcg gtgaggaggc ggagcgtgtt cacctcgagc ggcggcgggt cgatgatccg
     6421 ttcgaagccg taggccggga tgtcgtggct gccgcgaatc gcgagccatt tgtcgggatg
     6481 ggtcgccgcg agccggagga tcgtcccgac gacctgtcgg aacatcgggc cgaggtcggc
     6541 gccggggtcc tttgggtcgt ggaccttcgc cccgagccgg gcctggcaga cggcgaaccc
     6601 gccggtcagg gccgaggtcg tcatccagat gtcgatgccg aacttgctga tgtcctcggt
     6661 ccagtcgtcg agcgtcagat agtgcttgac gagatcgccg ctgaccccga agtcgccccc
     6721 gatcggctgg cggatccggt tgccgtagag ggctcgggtc agcgggtagg tgacggtgtt
     6781 ggtgatcgtc ccgtcgtact tgtagcgggc gtagagcggc gcaacgtagt cgtagccgcc
     6841 cttgaggatc ggcccggcca ggagctcgat ccattcgggg atgatcgagc gcaggtcgct
     6901 gtcgacgacg acgagcgcct ggacctcgag ggccgcggcg atctcgaaga tcgtccgcag
     6961 ggctgccccc ttgccaccga cgccgtcgat ctcggggtag gtcaggctca cccgcgagag
     7021 cttgttcgtc gggcgcacga ggaggatctg ctcgacatag tcgggcggtt cggtgtcgat
     7081 cacgacccgg ctcgtgccgt caggcgagcc ggcgtcgctg ttgaccagca cgggccgcag
     7141 gtccgggaag tactggacga gaccggcctg cgccgcgcgc acgacatagc cgatcgtggc
     7201 ggcgttcttg aagctcggga tgccgaccat gatgtcggcg tggcccagcc gggcgatctc
     7261 gtcgcggacc gccggcgtca atccttgggc aggcaccacg tgaccgtacc accgatcggc
     7321 gactgtcgcg cctggacgac gctggcggcg ttgccgcctg cggtcctcgc tcacagccat
     7381 ccgaggctgc tcgctccggt gctcgcggcc gctttgcgct gacgcactca tgcgcgacga
     7441 acgcagtcac ggggccgatt cggatcggcg actgtcgcgc ctgggcacgc atcgcggcgg
     7501 tgcgccctgc gctccgcgcg acttcgggcc gatggattcg cggagctcct ccgcgggctt
     7561 cggtgagccg gcccgaggtc cgctctcgca gactcgcggc acggagcgat gatgtcagcg
     7621 ggcggggatc tgcgcgaggg cccactccgg gttcttctgg ccgggcacgt agcggctgtt
     7681 cgcgcccttg taggccagcc gccggaagcc gaacgagcgc cacgatccga gccacgcgtc
     7741 gatcggcggc ttgacgtgga ttccgacccg gatgaggtcg ctctccggga ggactgactc
     7801 gaggatgcgc ttgcgctcga gctgcgggac gtcgcagagc gactcgtcgt cgagccagag
     7861 caggtcgacc gcgaccaggg cgaggcgatc cgtggtgcta tcgacggtcc actggcgggc
     7921 ctcttcctga cgcttcacga gccggtcctt ccggtcgccg cgggtcccga cgagcatccg
     7981 ggtcatcgcc tcgccgggct tcgggagcgt caccgggtcg cgggctgcga tgagcgcctg
     8041 gtcctggaac ggctcggggg tcaggtacgc ctccacgagg gccgttccac ccgccaccgc
     8101 ctcgcgaagg gcgtcgatga tgtccggctt gctgtcgacg agaccacctt cgatgtccgt
     8161 cagttggatc gtcgggccgt cgacgaaggc aaggatccgc gggccgctcc agagcggctc
     8221 gatgatcggg tccacgatcg aattcgcgcg tttgccgccg aacagctggg ggcgccaggt
     8281 cgctgcagcg gacgcggcat cggtggcgat catcgaccga tgatacgcgt cgatccctcg
     8341 gctgcgttca cgggtgctcg ccgtcgcgac ccggtcgatg ggccgacccg ctcggcgccg
     8401 attccaccgc cacgtgcccg cggcgcgcgc ctatgatccg gcgatggctg ccagcaagga
     8461 cgtccctgtc gcccgctccg ggcgcgggct cggcgccctc agcccgagcg gccgcgcggc
     8521 cgagccattc gcctggtcgc tgtacgactt cgccaacacg atcttctcgt acgccgtggt
     8581 gtccaccgcc atcgggctct gggcgaccga cgggagccgc ttcggcgaac gtgacggcaa
     8641 cctcgtcgtc tcgatcgcgg tcgcgatcag cgtcgccctc aacgcgatcg tctcgccgat
     8701 cctgggcgcg ctcagcgacc gcggcggacg gcgcctgccg tttctcctgt tcttcaccgt
     8761 gctctgcatc gtcccgaccg cgctcatcgg gccgagcccc gcgatcgtcg gaatcgccct
     8821 gttcgccgtc gccaacttcg gctaccaggc cgcgctgatc tactacgacg caacgctcaa
     8881 gacggtgagc tggcccgaga cgcgcggccg gctgagcggg atcggggtgg cggtcgggta
     8941 ctgcggcacg atcttcgtcg ggttggtgct catcctcctg aaggtgccga tcgaaggccg
     9001 cttcctcgtg gccgcgatcc tctttgccgt gttctccgtg ccgatcttcg tcgtcgtcaa
     9061 ggagcaggga ggggtcggtc ggatcagccg ctcggacgtg accggatcgt ggtcgcagct
     9121 ccggacgacg atcgatcacg cgcgcgccgt gcccggtctc ggacgcttcc tgctgggacg
     9181 cttcttctac tccgacgcgg tcaacacgat catcgtggtc atgaccgtgg tcacggtcta
     9241 cgccaaggga ctgagcgagt ccacggccca gatcgtcctg ctcggactga ccgtcgtcgc
     9301 cgtcctcgcc agcttcggtt ggggtcggct cgtcgacgcc tgggggccga aacggacgct
     9361 gatggtgatc ctcgcgtcgt gggccgtcgg gctcgtgctt ggcgccgtgt cgctcggcgt
     9421 cgacgggccg atcggcttcg tcgcgttcct ggtcgccggg gcgatcctcg gcagcgggct
     9481 cggcggcgtc caggtcgccg accgggtgtt catggtccgc ctctcgccgc ccgagcggct
     9541 gggcgagttc ttcggtctgt acggcctcgt cgggaagggc tcgcaggtca tcggccagat
     9601 gctctacggg ctgaccctgt tcctcctgct cgacacgttc aaggtcgggg cgtaccagct
     9661 cgcggtgctc acgcttctcg tgacgatggc catcggctac cggctgatcc agccggtctc
     9721 cgacacgtgg gccggcgccc acgagacgga cggcacgccg gcgccgcccg agcggctcgc
     9781 cccgtctgcg gccccgatcg agccgcgctg aggtctgatc aggcgatccg cgcgccggtc
     9841 gcgtcgaggt gggcccgtcg ccacgcccag gc
//
LOCUS       DATAIW010000364         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_53629, whole genome shotgun sequence.
ACCESSION   DATAIW010000364 DATAIW010000000
VERSION     DATAIW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53629"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VHM48_10975"
     CDS             <1..191
                     /locus_tag="VHM48_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755979.1"
                     /translation="FVPVVDAVAMFGDQRVGDPDVETILVNRFYLRGVEQIDKRTGLK
                     PQKLPGSPLGGITWQDRS"
     gene            complement(432..1676)
                     /locus_tag="VHM48_10980"
     CDS             complement(432..1676)
                     /locus_tag="VHM48_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2755980.1"
                     /translation="MARPAIVIALPPGERGPVTGELRAAGFEAISIGMPDELEALLAA
                     RHDIAVAILDGENDFDMSLEYYGLLHEGARNIPALMVMSPQAIDKMFNPSSADEFFSR
                     PYSADSIRWRVEAMCIRSVTIDDGSGPIIQGGSMEMGDWSRRATVIAIFNPKGGVGKT
                     TIATNLAAALQLHYHEKVLLVDADTVTGHVTTSLGLEQVRTVADSWREQVDDGIAESL
                     TELATPHPSGMSVVSLTSSPLNTEILEPTRIADAITTSRPTYDFIIVDLHPSYSALNR
                     AIFEKADKILVPVTPDVPALRAAVQLREVAIELGVQDRLALIVNRANSGVSVADMEKT
                     VGMPALALIRSGGLLFVRAANEGRTVIELYPRERITEDFDALAERLVRTFHTETLAQP
                     VVAQKAGGFRLPFGRAKEAARA"
     gene            complement(1740..>2234)
                     /locus_tag="VHM48_10985"
     CDS             complement(1740..>2234)
                     /locus_tag="VHM48_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755981.1"
                     /translation="AVADVAIEIMGNHEDEGEADPRFAALGLTPDYDAAEAEALAAAG
                     TSDEEIPEIGDDALAARLAGLVPGKNKGNAADSATTQVVVVGLVSVASIASFKRHLGR
                     VPGVQSVGVSSGPDGEFIFAVNHGADVVLRDAIPSLPSFQARVTGSSDGVIHVTAHDP
                     EADA"
BASE COUNT          341 a          734 c          800 g          359 t
ORIGIN      
        1 tgttcgtgcc ggtcgtcgat gcggtggcca tgttcggcga tcagcgggtc ggcgaccccg
       61 atgtcgagac catcctcgtg aaccgcttct acctccgggg cgtcgagcag atcgacaagc
      121 ggaccggcct caagccccag aagctgcctg gctctccgct cggcggaatc acctggcaag
      181 accgctccta gtagacgcct cccagggcgg ctccaccagt cgtgaccctg aaggcagtcg
      241 ttttcgatgt cggggagacg atcgtcgacg agacgcgggc ctggtcggcc tgggccgact
      301 ggcgctcagc cgctcaacat cgtcatcagg gcgccgaccc gcctgggctg aaccggcaac
      361 cggaacgcgg agtcagctcc cgcccaccga ggtccgaggc cgagttccgt cgaggccatc
      421 ggcccgaggt ctcaagctcg ggcggcttcc ttcgcgcgac cgaacggcag gcggaatccg
      481 cccgctttct gggccacgac cggctgggcg agcgtctcgg tgtggaacgt ccggacgagc
      541 cgctcggcca gagcgtcgaa gtcctcggtg atccgctccc gcgggtacag ctcgatgacc
      601 gtccgaccct cgttggctgc gcggacgaag agcaggccac ccgatcggat cagggcgagc
      661 gccggcatcc cgaccgtctt ctccatgtcg gccaccgaca cgccgctgtt ggcccggttg
      721 acgatgagcg cgagccgatc ctggacgccg agctcgattg cgacctcgcg gagctggacc
      781 gcagcgcgaa gggcgggaac gtccggagtc accggcacga ggatcttgtc ggccttctcg
      841 aagatcgccc ggttgagggc gctgtacgac gggtggaggt cgacgatgat gaagtcgtag
      901 gtcgggcgtg acgtcgtgat cgcgtcggcg atccgcgtcg gctcgaggat ctcggtgttg
      961 agcggcgacg acgtgagcga tacgaccgac atccccgacg gatgcggggt ggccaattcg
     1021 gtcagtgatt cggcaatgcc gtcgtcgacc tgctcacgcc agctgtcggc cacggtccgg
     1081 acctgctcga gcccgagcga ggtcgtcacg tggccggtca ccgtgtcggc gtcgacgagg
     1141 aggaccttct cgtggtagtg gagctggagg gcggccgcga ggttcgtcgc gatcgtggtc
     1201 ttgccgacgc cgcccttcgg attgaagatc gcgatgaccg tggcccgtcg agaccagtcg
     1261 cccatctcca tggagccgcc ctggatgatc gggcccgagc cgtcgtcgat ggtgaccgaa
     1321 cggatgcaca tcgcctcgac acgccagcgg atcgagtcgg ccgagtaggg tcggctgaag
     1381 aactcgtctg cggagctcgg gttgaacatc ttgtcgatgg cctgcggcga catgaccatc
     1441 agggcgggga tgttccgtgc tccctcgtgg agcaggccgt agtactcgag cgacatgtcg
     1501 aagtcgttct cgccgtcgag gatcgcgacc gcgatgtcgt ggcgggccgc caggagggcc
     1561 tcgagctcat cgggcatgcc gatcgagatc gcctcgaagc cagccgcccg gagctcgccg
     1621 gtgacgggcc cgcgttctcc cggcggaagg gcgatgacga tcgctggtcg tgccacgatc
     1681 tatgactccc cgggttctcg cggtggtgag gtggacgcgg gggggccggg cccggccggt
     1741 tacgcgtccg cctccggatc atgagcggtg acgtgaatca cgccgtcgga cgagccggtg
     1801 acgcgggcct ggaagctcgg cagggacggg atcgcatcgc ggaggacgac atcggcgccg
     1861 tggttgacgg cgaagatgaa ctccccgtcg ggccccgacg agacgccgac cgactggaca
     1921 cccggcaccc gcccgaggtg gcgcttgaag ctggcgatgc tcgcgacgct gacgagcccg
     1981 acgacgacaa cctgcgtcgt cgccgagtca gcggcgttgc ccttgttctt gcccgggacg
     2041 agacccgcca ggcgagcagc gagcgcgtcg tcgccgatct cggggatctc ctcgtccgac
     2101 gtgccggcgg cagcgagggc ctcggcttcg gcggcgtcgt agtcgggcgt caggccgagg
     2161 gccgcgaagc gcggatccgc ctcgccctcg tcctcgtgat tgcccatgat ctcgatcgcg
     2221 acgtcggcga cggc
//
LOCUS       DATAIW010000365         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_53667, whole genome shotgun sequence.
ACCESSION   DATAIW010000365 DATAIW010000000
VERSION     DATAIW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53667"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VHM48_10990"
     CDS             complement(<1..635)
                     /locus_tag="VHM48_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755982.1"
                     /translation="MTEPREPASDVTEPIAIPPDGADASAVAPGAMPEADTDAARAEA
                     EAAAMAADELGGEDEPDGAEDDDEPAEDEAEGDEDEEADEPADDEGIGDESAAGAAAA
                     LASGSVPGRVRVGRATKPTKVVTPATIIDPSIKVTDQASKIFVIGTVLVFVLIFAYGA
                     LLGVGGSFTPYVAPTPSPTPIATPTPRATRTARPRTTARATASGWRSASATV"
     gene            complement(742..>1873)
                     /locus_tag="VHM48_10995"
     CDS             complement(742..>1873)
                     /locus_tag="VHM48_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755983.1"
                     /translation="AYVDRWNQRDVLVVSDILRAAVVLIMPIAAVTNVYLVYPLIFLV
                     TSISIFFRPARVAVLPRIVDEDDLLTANSALWAGETFADIIGYPLAGLFVGFLGAALP
                     LAFWIDAATYGASAILIASIAIPTVQRTRHSAMVQPVAAVAARPIEAGSENDDPGARA
                     GHDETAEAPSGRRSVLADMREGYDFLRHEPVLFANTIQAAFAQFAIGALTALTPIFVR
                     DFLGPANGIDPKAAYSFLEASIGIGNLVGGFAIGLVGMHLAKGRLVIGGYVLFGLFLI
                     GFALSGSLVVDFGLLLGVGIANMIFVIPSQVLFQQRTPQELIGRVVSFRFALVFGSMT
                     MAMAIGGVLGQVVGIVPVLVVSGIASILAGLAGLLLPAMRDA"
BASE COUNT          292 a          656 c          665 g          260 t
ORIGIN      
        1 accgtcgccg acgccgatcg ccaccccgac gccgtcgccc gcgccgtcgt cctcggtcgc
       61 gccgtccggg tcgcccgcgg cgtcggggtg gcgatcggcg tcggcgacgg tgtcggtgcg
      121 acgtacggcg tgaacgagcc gccgaccccg agcaatgccc cgtaggcgaa gatcaggacg
      181 aagacgagca ccgtaccgat gacgaagatc ttcgacgcct gatcggtgac cttgatcgaa
      241 ggatcgatga tcgttgccgg cgtcacgacc ttggtcggct tcgtcgcccg acccacgcgc
      301 acacgaccgg gcaccgaacc cgaggcaagc gccgccgcgg cgcccgcggc cgactcgtcg
      361 ccgatccctt cgtcgtccgc gggctcgtcg gcttcctcgt cctcgtctcc ctcggcttcg
      421 tcctccgcgg gctcgtcgtc gtcctcagcc ccgtcgggct cgtcctcgcc gccgagctcg
      481 tcggccgcca tggcggccgc ctcggcctcc gcccgggccg cgtcggtgtc ggcctcgggc
      541 atcgcgccgg gcgcgaccgc agatgcatca gcgccgtcgg gcgggatcgc gatcggctcc
      601 gtcacgtcgg acgcaggctc cctcggttcg gtcatccggt cgtgtctctc ctgtgctgga
      661 cgctcggcgg ctcgagccgc gctggccggc gtgttggatc gcggcagcgg cgtcggggtg
      721 cggccgccag tgtaccgaag gtcaggcgtc gcgcatcgcg ggcaggagca ggccggccag
      781 gccggcaagg atcgaggcga tgccgctgac gacgagcacg ggcacgatcc ccacgacctg
      841 gccgagaacg ccaccgatcg ccatggccat cgtcatcgac ccgaacacca gcgcgaaccg
      901 gaagctcacg accctcccga tcagctcctg tggggtccgc tgctggaaca ggacctggct
      961 cgggatcacg aagatcatgt tcgcgatgcc gacgccgagg agcagcccga agtcgacgac
     1021 caggcttccg ctcagggcga acccgatcag gaacaggccg aacaggacgt agccgccgat
     1081 cacgagccgg cctttggcga ggtgcatccc gaccaggccg atcgcgaacc cgccgacgag
     1141 attgccgatc ccgatcgacg cctcgaggaa cgagtaggcc gccttcgggt cgatgccgtt
     1201 ggccggcccg aggaaatcgc ggacgaagat cggggtcagg gcggtcaggg cgccgatcgc
     1261 gaactgggcg aaggccgcct ggatcgtgtt ggcgaacagg acgggctcgt ggcgcaggaa
     1321 gtcgtatccc tcgcgcatgt cggcgaggac gctcctccgc ccggaagggg cctcggccgt
     1381 ttcatcatga ccagccctcg cgcccgggtc gtcgttctcc gagcccgcct cgatcggccg
     1441 ggcagcgacg gcagcgaccg gctggaccat cgccgaatgc cgggtccgct ggacggtcgg
     1501 gatcgcgatc gacgcgatga ggatcgcgga ggctccgtag gtcgccgcat cgatccagaa
     1561 cgccaggggc agggcggccc cgaggaagcc gacgaagagg ccggccagcg ggtagccgat
     1621 gatgtcggcg aaggtctcgc cggcccacag cgccgagttg gcggtcagca ggtcgtcctc
     1681 gtcgacgatc cgtggcagga ccgcgacccg ggccgggcgg aagaagatcg agatcgaggt
     1741 gacgaggaag atcagcgggt agaccaggta gacgttcgtg actgccgcga tcggcatgat
     1801 caggacgacc gccgcccgga ggatgtcgct cacgaccagc acgtcgcgct ggttccagcg
     1861 gtcgacgtag gct
//
LOCUS       DATAIW010000366         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_129418, whole genome shotgun sequence.
ACCESSION   DATAIW010000366 DATAIW010000000
VERSION     DATAIW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129418"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1354)
                     /locus_tag="VHM48_11000"
     CDS             complement(95..1354)
                     /locus_tag="VHM48_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX2755984.1"
                     /translation="MSSDPDELITVLDAAELGPRRPVGVLRRRPGRRTVISFEYARSW
                     LDGPGSFAIDPSLALGPAERFFGDGLLPGVLSDTAPDRWGRGLLERREAAAAREEGRP
                     IRVLDDWDFLVGVADETRMGALRIGRTPSGPFLDGSRGVPPMTRLRSLEFAASELERS
                     PHTPIDDPAVALLIAPGSSLGGARPKANFVADDGDLWIAKFPSRQDRRDVGRWEYLLI
                     LLARDAAITVPECRTLSLSTAGTTFATRRFDRHGADRRLYASAMTLTGKHDGDEASYL
                     DLALAVADHVDPASIAADLEQLFRRLVFNVMVGNRDDHLRNHGFLRTGAGWRLAPAFD
                     LNPSPDVFEHSLAIDEGSHEPDLAVAIQTCPLYRLSNSDAVRVVRDVAKAVAGWRSRA
                     RAIDLPGEEIDRMASVISATSVESSSR"
     gene            complement(1344..1691)
                     /locus_tag="VHM48_11005"
     CDS             complement(1344..1691)
                     /locus_tag="VHM48_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2755985.1"
                     /translation="MVIVCFMTRPARPAFPAMIRRAEALGGRLRLARLRRRMTESDMA
                     ARVGVSRPTIRRLEAGSLAVSYGVLARVLEVLSLGDQIDLIASEDDLGEQLNDSRLGR
                     PRRSSRSTLADEL"
     gene            complement(1923..>3899)
                     /locus_tag="VHM48_11010"
     CDS             complement(1923..>3899)
                     /locus_tag="VHM48_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755986.1"
                     /translation="IRFALAVLALAPATVLMGATLPTLTRQLSQDRHLSAAFGRLYAA
                     NTLGAIVGTLVAGLLLIELFGLTGTLVIGAACSATAGAVALLLSRGAEPVAPVAVPAS
                     VRWSAKQAEAAEARRPTLALLLAFVSGLTSLGYQVLWTRLLSSGTGNSTYVFTLILGM
                     FLIGITIGATLFAITRRWIQRPLALIAGAQVVVGAIAIAGLVLVIGKPGPIDPGKALE
                     AAQAILFPIILVVLPATIVMGFTFPAASTLLGDDPARIAGNAGRLLAANTAGAIIATF
                     AIPFFVIPAVGSPNAVALLALVNVATAVAIVISSSGREALSRTARLATSAGALVVTVA
                     IVSGLTTAGAIVDPSIARIHDVGAKLFASHEDEIASVQAGETVTAQLWVTGTAMTLLT
                     VDAKMMPVLPLTLRPDSKTAATVAFGMGSAFRGALIAGLKTEAVELVPSVPEMFPYFY
                     PDAAAVLANPNGKVIVTDGRNHIELTTETYDIIVTDPPPPIFSSGASVISSLEYYQAG
                     HARLNPGGIMMQWVPFGATIDEFQAHLRTFRSVFPHVTMVFGPGGYGLYMLGSDQPMV
                     FDDATTREVLGRPGILADISSAYDSPAKTIDAWVARIDSLRWIADDQVDRFVGSGPLI
                     TDDHPLPEYFLLRRLFNQKAPYAGPGLLLSMTRG"
BASE COUNT          658 a         1296 c         1409 g          536 t
ORIGIN      
        1 actgagaaca gcaggaacgc cgaccagccg atgagcccac cgaaggcgag cacggtcgaa
       61 cgtcgttgcc ggcgttctcc gtggtcgcgg gctgtcacct cgacgacgac tcgacgctgg
      121 tcgccgagat gactgacgcc atccggtcga tctcctcgcc cggaaggtcg atcgcccgcg
      181 ccctgctccg ccagcccgcg acagccttcg cgacgtcacg cacgacccga acggcgtcgg
      241 agttcgagag ccggtacaac gggcacgtct ggatggccac cgcgagatcg ggctcgtgcg
      301 agccttcgtc gatggcgagg gagtgctcga acacgtcggg ggaagggttc agatcgaacg
      361 cgggcgcgag tcgccagccg gcgccggtcc ggagaaagcc gtggttccgg agatgatcgt
      421 cgcgattgcc gaccatcacg ttgaacacca ggcgccggaa gagctgctcc agatccgccg
      481 cgatcgacgc cggatcgaca tggtccgcga cggcaagggc gaggtcgaga tagctcgcct
      541 catcgccgtc gtgcttgccg gtgagggtca tcgccgaggc gtacaagcgg cgatcggctc
      601 cgtggcggtc gaaccgtcgc gttgcaaagg tcgtgccggc tgtcgacagg gacagggttc
      661 tgcattccgg gaccgtgatc gcggcgtcgc gagccagcag gatgagcagg tactcccagc
      721 gcccgacatc ccgtcgatcc tgtctcgacg ggaacttggc gatccacaga tcgccatcgt
      781 cggcgacgaa gttcgccttc ggtcgggccc cgccgagcga gctgcccgga gcaatgagga
      841 gcgcgaccgc tgggtcgtcg atcggggtgt gcggggatcg ctcgagttcc gatgcggcga
      901 actccagcga acggaggcgg gtcatcggtg ggacgccgcg gctgccatcg agaaacggcc
      961 cgctcggcgt ccgaccgatg cgcagagcac ccatccgcgt ctcgtcggcg acgccgacca
     1021 ggaagtccca gtcgtcgaga acgcggatcg gacggccttc ctcgcgcgcg gcggccgctt
     1081 cgcgacgctc cagcaaaccg cgaccccaac gatccggggc ggtatccgag agcacgcccg
     1141 gcagcaggcc gtcgccgaaa aatctctcgg ccgggccgag ggccagcgaa gggtcgatcg
     1201 cgaacgagcc cggcccgtcc agccaggatc gcgcgtattc gaagctgatg acggtccgtc
     1261 gtccgggtcg cctgcgcaga acgccgaccg gtcgccgcgg cccgagctcg gcggcgtcga
     1321 gcaccgtgat cagctcatcg gggtcagagc tcatcagcca gagtcgaccg gctcgatcgc
     1381 cggggtcgac cgagcctcga gtcgttcagc tgctcgccga gatcgtcctc gctggcaatg
     1441 aggtcgatct ggtcgcccag cgacaggacc tcgagcaccc gggcaagcac cccatagctc
     1501 acggcgagcg atcccgcctc gagacgacga attgtcgggc gcgaaacacc gaccctggcg
     1561 gccatatcac tctcggtcat cctccgccgc aaccgggcga gtcgaagacg tccgccgagt
     1621 gcctccgcgc ggcggatcat tgccgggaag gcaggccgcg ccggtctggt catgaaacat
     1681 actattacca ttaagaccgt tactgtcaat agtatgttct ggcgtctacg ggacctgcaa
     1741 cgtcagcgct gatcgacagt ccgccgccca cgcgctgcgg atagccgaat gattcgagat
     1801 tcgcagtatt cggctattcg aagggccatg gcggacgaag tccggcgcga ggtgggcgcg
     1861 aggtggacac gaggttcggc gcgaggtgga cacgaggttc ggcgcgagac gccctcgtgc
     1921 cctcaaccgc gggtcatcga gagcagcaga cccgggccgg cgtacggcgc cttctggttg
     1981 aacagccggc gcaggaggaa gtactccggc agcgggtggt cgtcggtgat cagcggaccc
     2041 gaaccgacga accggtcgac ctggtcgtcg gcgatccagc gcaggctgtc gatccgggcg
     2101 acccaggcgt cgatcgtctt cgccggcgag tcgtaggcgg acgagatgtc ggccaggatc
     2161 ccgggccggc cgaggacctc gcgggtcgtc gcgtcgtcga agaccatcgg ctggtcggag
     2221 cccagcatgt acagcccgta gccccccggc ccgaacacca tcgtcacgtg cgggaacacc
     2281 gaccggaacg tccgcaggtg ggcctggaac tcgtcgatcg ttgcgccgaa cgggacccac
     2341 tgcatcatga tcccgcccgg gttcaggcgg gcgtggccgg cctggtagta ctcgagcgac
     2401 gagatcaccg acgcgcccga gctgaagatc ggcggcggcg ggtcggtgac gatgatgtcg
     2461 tacgtctccg tggtcagctc gatgtggttg cggccgtcgg tgacgatcac cttcccgttg
     2521 gggttggcca ggaccgcggc ggcgtccggg tagaagtacg ggaacatctc gggcaccgac
     2581 ggaacgagct cgacggcctc ggtcttcagg ccggcgatca gggcgccgcg gaacgccgag
     2641 cccatcccga acgcgaccgt cgccgcggtc ttggagtcgg gccggagggt gagcggcagg
     2701 accggcatca tcttcgcgtc gaccgtgagc agcgtcatcg cggtcccggt cacccagagc
     2761 tgggcggtca ccgtctcgcc cgcctggacg gacgcgatct cgtcctcgtg gctcgcgaac
     2821 agcttcgcgc caacgtcgtg gatccgggcg atgctcggat cgacgatggc accggccgtc
     2881 gtcaggccgc tgacgatcgc gaccgtgacg acgagcgcgc cggcgctcgt cgcgagacgg
     2941 gccgtccgcg acagcgcttc gcggccggag ctgctgatga cgatcgcgac cgcggtcgcg
     3001 acgttgacga gggcgagcag ggcgacggcg ttgggcgagc cgaccgccgg gatcacgaag
     3061 aacgggatcg cgaacgtcgc gatgatcgcg cccgcggtgt tggcggcgag gagccggccg
     3121 gcattgccgg cgatccgggc cgggtcgtcg ccgagcagcg tcgacgcggc ggggaatgtg
     3181 aagcccatga cgatcgtcgc cggcaggacg acgaggatga tcgggaagag gatcgcctgg
     3241 gccgcctcga gggccttgcc gggatcgatc gggcccggtt tgccgatgac gaggacgagc
     3301 ccggcgatcg cgatcgcgcc gacgacgacc tgggccccgg cgatcagggc gagcggccgc
     3361 tggatccagc gtcgggtgat cgcgaacagc gtggcgccga tcgtgatccc tatcaggaac
     3421 atcccgagga tcagcgtgaa gacataggtc gagttcccgg tgcccgacga caggaggcgc
     3481 gtccacagga cctggtagcc gagcgaggtc aggcccgaca cgaaggccag caggagcgcg
     3541 agggtcggcc ggcgggcctc ggcggcctcg gcctgcttcg cgctccagcg cacggaggcc
     3601 ggcacggcaa ccggcgcgac cggctctgcc ccgcggctga gcaggagggc gaccgcgccc
     3661 gccgtcgccg agcacgccgc cccgatgacg agcgtgccgg tcagcccgaa cagctcgatc
     3721 aggagcagcc cggcgacgag cgtcccgacg atcgccccga gcgtgttcgc cgcgtacagc
     3781 cggccgaacg ccgccgagag atggcgatcc tggctgagct ggcgggtgag cgtcgggagc
     3841 gtggcgccca tcagcacggt cgccggcgcg agggcgagga ccgccagggc gaaccggat
//
LOCUS       DATAIW010000367        12541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_113495, whole genome shotgun sequence.
ACCESSION   DATAIW010000367 DATAIW010000000
VERSION     DATAIW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113495"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VHM48_11015"
     CDS             <1..816
                     /locus_tag="VHM48_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2755987.1"
                     /translation="QLAPVPYLATVAALTERMRISAFVHNNDLRHPAVLAQELASLDV
                     LSGGRLDVAIGAGWNEPEYQAIGLPFDPIKVRQARLAEAITVIKGCFGPEAFSFSGEF
                     YTITEYDAYPKPVQEPHVPFLIGGGGRSTLELAAREADIVGLAPRILSGQRPDPRSIT
                     WTATEEKIGWVREVAGHRFGGLSFNVYPSQWPITVTDDLHGEARKVIDRMKERTGVEM
                     TEQEVIDSPHIFIGSIDRFVDKFSELRERLGINSFLVGSLDELGPVVERLAGT"
     gene            complement(841..1356)
                     /locus_tag="VHM48_11020"
     CDS             complement(841..1356)
                     /locus_tag="VHM48_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755988.1"
                     /translation="MRPADIAALFDHLYWVRDRVLAASNDPAAPFIDQASPTLRDLRT
                     TLVHELDVEWSWRERLAGPDPTSFSADDEDLVATDFANVAAIRDRWATDEAAMRTWLA
                     SLDKAALAASCRAEGSGGRHPLWFHLQHLYSHAIQQFADAAVVLTAAGRSPGELDFLQ
                     FVEDRERSAGS"
     gene            1672..2043
                     /locus_tag="VHM48_11025"
     CDS             1672..2043
                     /locus_tag="VHM48_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEX2755989.1"
                     /translation="MSRTQRQRGHDRRAETFRCIHCRLDVPLAAAGTAHRNHCPGCLW
                     SRHLDVDPGDRASDCQASMEPIAITVRRDGEWVLIHRCQGCGTLSSNRTAGDDNPLLL
                     VRIAVKPLAQPPFPLDRLALL"
     gene            2162..3214
                     /locus_tag="VHM48_11030"
     CDS             2162..3214
                     /locus_tag="VHM48_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2755990.1"
                     /translation="MTDGQITRVGFIGAGVMARSHLADMLARDDTTVAAVCEPSPAAY
                     AQVVELFDRHGRAAPANEPDWQRFVATFADQLDAVFILTPHAFHFAQATACLEAGLDV
                     LLEKPMVLTADEADALIATRDRTGRLLVVAFQGSLSPQVREASRLLRSGELGSILNIS
                     ATAWQNWSTITTGTWRQEPVLSGGGFLFDTGAHMLNTVADLAGEDFAEVAAWLEDDGR
                     PVDIRATVMGRLVSGALVTMNACGSAIPSCHSEIRVFTSGAILKTGIWGETLEIQRLG
                     ASGLRKVRAVAGIHVWEQFLNVRSGRIPNPSPPEVGLRMARLWDAIRASAAMAGAVVR
                     LPASEPVLTGTAAAGR"
     gene            3211..3933
                     /locus_tag="VHM48_11035"
     CDS             3211..3933
                     /locus_tag="VHM48_11035"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX2755991.1"
                     /translation="MTRVTVWSEHRQERSDPPVAAIYPDGIHGAIAAGLRDAGFEVRT
                     ATLDEPEHGLTEAVLAATDVLTWWGHVAHDEVDDAIVDRVQRRVLDGMGLIVLHSGHH
                     SKIFRRLMGTTADLKWREAGERERIWVVDRSHPIADGLGDSFVLDEEEMYGEAFDVPA
                     PDQLVLISWFQGGEVFRSGCCWSRGRGRVFYFRPGHETYPTYFQPMVRRVIANAVSWA
                     VGPRGAPIVVANQDAPEGLERG"
     gene            complement(3951..4475)
                     /locus_tag="VHM48_11040"
     CDS             complement(3951..4475)
                     /locus_tag="VHM48_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2755992.1"
                     /translation="MPASPPRPEPATLEADRSGPGRGAVEAIHVTSGGGEPMHSIERV
                     RAIAGVGLEGDRYATGAGHYSGDTKVDRHVTLIEAEEIEALAVGSGITLEPGATRRNV
                     TTRGIRLNELVGRRFRIGAVACEGTRLCEPCQYLTDLLGKPVLTALVHRAGLRARILT
                     DGDIALGDEVVVLG"
     gene            4547..6028
                     /locus_tag="VHM48_11045"
     CDS             4547..6028
                     /locus_tag="VHM48_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2755993.1"
                     /translation="MTDGTHPNRAHDDGLWSPGRRALTIGLVLNVTIVASEALAVGTI
                     LPIVAKDLGGIDLYGWVFSGFFLGNLLGIVVAGILIDRGGLTRPFLMGIGLFAIGLLV
                     GGLAPSMEILVIARLIQGFGAGAIPAVAYVSIGRAMPAHLRPRMFATLSTAWILPGVI
                     GPVAASAVTGLLNWRIVFLGLLPLIAIAATLTLPAIRAVAAAPAAAAGTPASARIAAG
                     RVRLPLAFVLVVGAGLVVSGLSNVSPIPLVLIVIGIAIGAPAFARLTPRGTIRAATGL
                     PTAILLRGFLTFAFFCADAYVPLVIQGWRGDSAYMAGFTYMAATLAWTGGSWVNAHHI
                     GRLGPGRFLRVGFVVVLVGIVGFLAVLLPWVPPAAGIVIWGVAGFGMGLAYSTPSLIV
                     LSEAPAGEQGAATAGLQLSDVLGASLGTGIGGALIAFGSRAGSPGWEGLAATFAVAGV
                     VALIGLALTTRLGARTAAGLPPTHLGTATAWATSETGRDPAVR"
     gene            6096..6617
                     /locus_tag="VHM48_11050"
     CDS             6096..6617
                     /locus_tag="VHM48_11050"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX2755994.1"
                     /translation="MSAEELRAKIREIPDFPKPGILFYDITTLLKDRAAYKEAIDLML
                     APYQGEKIDVVVGMESRGFIFSSAMAYQLKAGLVPVRKLGKLPAETITVEYALEYGSN
                     TLEIHRDAIEPGQKVLIVDDLLATGGTVKGTIELIERLKGDVIGLAFLVELDFLKGRD
                     RLDGRRVTSVIKY"
     gene            6677..8239
                     /gene="mtnA"
                     /locus_tag="VHM48_11055"
     CDS             6677..8239
                     /gene="mtnA"
                     /locus_tag="VHM48_11055"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX2755995.1"
                     /translation="MADAIRDSDHDPPAAPDDDGSAVVDPAGAVDPAAVHISTGAVEP
                     AADGDPTNLARRRFFRQFAGELIQTAATVAGAAQALQRASAEAAGAILDPVAAAARLE
                     AAPVSVGTTASGLTDRGSAADSPGGPTGFRTPFREDDGVLHLIDQRKLPDQLVEVEVR
                     NAPEAATAIRQMVVRGAPAIGQVAAIGLALSADRSKTTQPFARRAILRGGAAALRAAR
                     PTAVNLGWAVERMMAAFEAVGDLSEDGPAVAAALRAEADAIVSEATTDHGRLAEFGLA
                     VLPVKEFGPLRILTHCNTGPLACGQFGTALGIVQAAHHAGREVTVWVDETRPYLQGAR
                     LTAWELAQAGVPHTLIPDVAAGFLMSRGEVDVILVGADRVAANGDTANKVGTYSLAVL
                     AARHRIPFYVCAPTNSVDLTTPDGDSIEIEERSADEVLLIRGVAIAPPGTAVRNPSFD
                     VTPAELITGIVTEEGVVTAPYEAGLGAAIAAAKLRWAPRPLIPPTPRPPVERADDGAT
                     AAGPGITTGVPD"
     gene            8239..9102
                     /locus_tag="VHM48_11060"
     CDS             8239..9102
                     /locus_tag="VHM48_11060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2755996.1"
                     /translation="MASVAATGRRARGFTIRSVTDRGQLRAFLETDRLLAAYAICDLE
                     DKEFGKTRWGGAFEGDRLVALALEYSGLTPQPLFVMGRAEGVEAILRDVVRPRAAYVA
                     VLPEALPAVETVYRLDPGPQMVRMWVDRGRFRPYPADVRRLLPADIGELNRLYQLGFA
                     SWLPSSSIADGVYFGIRVGGRLVAAAGTHVISREARLAVVGNVLTHVDYRGRGFATAV
                     TGAVTAELLRTSDQVVLNVRSDNPPALQAYRRLGYTEHVRFEERLAHRLGSPWPDFTA
                     PIRRLFSRREN"
     gene            9108..10421
                     /gene="ahcY"
                     /locus_tag="VHM48_11065"
     CDS             9108..10421
                     /gene="ahcY"
                     /locus_tag="VHM48_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2755997.1"
                     /translation="MTDTAAPPQGAPPPATAGLPAHDVTDLGLASEGVRRIDWAEREM
                     PVLRLIRERFEREQPLAGIRIGACLHVTTETANLMRTLKAGGAEVFLAASNPLSTKDD
                     VAAALVAEYGIATFARRGEDGDTYYAHLNAIADTKPQITMDDGCDLVALLHRERPAQV
                     KEVLAGTEETTTGVIRLKAMAADKALGFPVVAVNEAQTKHLFDNRYGTGQSTIDGILR
                     ATNILLAGRNVVVAGYGWVGKGIASRMSGMGAHVAVVEVDPVRALEALMDGFRVMTVG
                     EAAPWGQLFVTATGNVNVFRREHFAAMQEGAVMANSGHFDAELDLKALREMAEGHVRD
                     ARENVQEFDIGGRKINLIAEGRLVNLGAAEGHPAAVMDMSFANQALSAEYVVQHHAEL
                     TPQVYIVPEAIDAEVARLKLAAMGITLDDMTAEQKEYVSSWQHGT"
     gene            10426..12429
                     /locus_tag="VHM48_11070"
     CDS             10426..12429
                     /locus_tag="VHM48_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2755998.1"
                     /translation="MSDVHRAAPYGSWVSPFPIELLSRGAVTFGEIGASGGACWWLEG
                     RAEEGGRQVLVRREADGTLTRLTPEGFNARCRVHEYGGGAYLIDGDLVVVSDFTTGRL
                     QRVTAPGVLEPLTPAGKAWRFADLSLDRGRNRILAIREDHEPATIARHGEAENAVVAI
                     DLADGSVTVLAEGADFFSSPRLSPDGSRLAFLRWNHPNLPWDGTELCLADLGADGCPA
                     KERVVAGSPADWIAQPRWSPAGVLHFVAEPDGWMNLMRLGAGDRVERASEPIEAEFAF
                     PDWVFSLANYAIAADGTIIGAGRSGGRDRLYRIGPDGSATTFDLPFNEFGYVAIDGST
                     AVFRAAAADRPWAIVELDLATAATTELRRSIELVVDPADASLGELVEFPTEGDRTAFG
                     ILYRPKHQTCSGPVDARPPLIVTSHGGPTAEASTAFAVLIQLFTSRGLAVLDVDYGGS
                     TGYGMAYRKRLEGEWGVVDLADCVAGARWLAAEGIVDGQQLAIRGGSASGYTTLCAVT
                     FSDVFSAGTSYFGIGDLETFARTTHKFESRYLDRLIGPYPERKDLYHDRSPLNFADRI
                     ACPVLIMQGAEDRIVPPAQAEQIVDVLWERRLPHAYILFPGEDHGFRSAANIIRSFEA
                     ELSFYGQVFGFTPADAIEPIAVEFLDGGPATADGAGPRRATPA"
BASE COUNT         1726 a         4570 c         4366 g         1879 t
ORIGIN      
        1 cagctggcac cggtgccgta cttggcgacc gtggccgccc tgaccgagcg gatgcggatc
       61 agcgcgttcg tccacaacaa cgatctccgc cacccggccg tcctcgccca ggagctcgcg
      121 tcgctcgacg tcctgtcggg cggtcggctg gacgtcgcga tcggggccgg ctggaacgag
      181 cccgagtacc aagcgatcgg cctgccgttc gacccgatca aggtccgcca ggcgcgcctc
      241 gccgaggcga tcaccgtgat caagggctgc ttcgggcccg aggcgttctc gttcagcggc
      301 gagttctaca cgatcacgga gtacgacgcc tacccgaagc cggtccagga gccgcatgtc
      361 ccattcctga tcggcggcgg cggccggtcg accctcgagc tggcggcccg cgaggcggac
      421 atcgtcgggc tggcgccgcg catcctgtcc ggccagcggc ccgatccgcg ctcgatcacc
      481 tggaccgcga ccgaggagaa gatcggctgg gtccgtgagg ttgccggcca ccgcttcggc
      541 gggctgtcgt tcaacgtcta cccgtcgcag tggccgatca cggtgaccga cgacctccac
      601 ggcgaggcgc gcaaggtgat cgaccggatg aaggagcgga ccggggtcga gatgaccgag
      661 caggaggtca tcgactcgcc ccacatcttc atcggctcga tcgaccgctt cgtcgacaag
      721 ttcagcgagc tccgcgagcg gctcgggatc aactcgttcc tcgtcggcag cctcgacgag
      781 ctcggtccgg tggtggagcg gctggcaggg acctgacccg cccgcggagt cggcgggccg
      841 tcagctgcct gcgcttcgct cgcgatcctc gacgaactgg agaaagtcga gctcgccggg
      901 cgatcggccg gcggccgtga gaacgaccgc ggcatcggcg aactgctgga tcgcgtgact
      961 gtagagatgc tggaggtgga accagagcgg atggcggccg cccgatccct cggcccgaca
     1021 gctcgccgcc agtgcagcct tgtccaggct cgccagccac gtccgcatcg cggcctcatc
     1081 ggtcgcccac cgatcgcgga tcgcggcgac gttggcgaag tcggtcgcga cgaggtcttc
     1141 gtcgtcggcg gagaaggatg tcgggtccgg gccggcgagc cgctcgcgcc agctccactc
     1201 gacatcgagc tcgtggacga gcgtcgttcg gaggtcgcgg agcgtcggtg atgcttgatc
     1261 gatgaagggc gcggcaggat cgttgctggc cgcgagaacg cgatcgcgga cccagtagag
     1321 atgatcgaag agggcggcga tgtcggcagg tcgcacgccg tcagtgtggc gcatccccgg
     1381 ccgaacgtcc gttcgaccct tgacgtcatc cgggcacaac gtcatgctgc gccacggttc
     1441 gcagatcgca gggcccgggt ccagagccgg cgagccggaa aggagagcgt ttcatgttcg
     1501 cagccgccgt tgccgccgcg tccgcgtcac gcctccggga gaccgttcgc tcgtagctca
     1561 gcccgttgcc gagcccgaga ccgtccccgc ggggacggtc gttccatgtc cgggagcgtg
     1621 acgccgttcg atccgacggc ttccccctgc gtacccggag accgaatccg attgtcacga
     1681 acccaacgcc agcgcggcca cgaccgtcgc gccgagacct tccgctgcat ccattgccgg
     1741 ctcgacgtcc cgcttgccgc ggccggcacc gcccaccgca accactgccc gggctgtctc
     1801 tggtcgcgtc acctcgacgt cgatccgggc gaccgcgcgt ccgactgcca ggcctcgatg
     1861 gagccgatcg ccatcaccgt ccgcagggac ggcgagtggg tcctcatcca tcgctgccag
     1921 ggctgcggca cgctctcgtc caaccggacg gcgggcgacg acaacccgtt gctcctggtc
     1981 cggatcgcgg tcaagccgct ggcccagcca ccgtttcctc tcgaccggct cgcgctcctc
     2041 tagaagcgcg agccgcacgg gcacgacctg ggccggacgg gcaagacccg tcgcgcgtcg
     2101 atccggtagg gtcatcggcg acaaaacctg acgcgatcac agaccgagga gacggcagcc
     2161 gatgaccgac gggcagatca cacgagtcgg gttcatcggc gcgggcgtca tggcccgcag
     2221 ccatctcgcg gacatgctcg cccgcgacga cacgaccgtc gccgccgtat gcgagccttc
     2281 cccggcggcc tacgcccagg tcgtcgagct gttcgaccgc cacggccgcg ccgccccggc
     2341 caacgaaccc gactggcaac ggttcgtcgc gacgttcgcc gatcagctcg acgccgtgtt
     2401 catcctgacc ccccacgcgt tccatttcgc ccaggcgacg gcgtgtctcg aggccggcct
     2461 cgacgtcctc ctcgagaagc cgatggtcct caccgccgac gaggcggacg ccctgatcgc
     2521 gacccgcgac cggaccgggc ggctgctggt cgtcgccttc cagggcagcc tctcgccgca
     2581 ggtccgcgag gcgtcccgcc tcctccgctc cggcgagctc gggtcgatcc tcaacatcag
     2641 cgcgacggcc tggcagaact ggtcgacgat cacgaccggc acgtggcgcc aggagccggt
     2701 cctgtccggt ggcggcttcc tgttcgatac cggagcccac atgctgaaca ccgtggccga
     2761 cctcgccggg gaggactttg ccgaggtcgc ggcctggctc gaggacgacg gccggccggt
     2821 tgacatccgg gccacggtga tgggccggct cgtctccggg gcgctcgtga cgatgaacgc
     2881 ctgcggaagc gccatcccat cgtgccattc cgagatccgg gtcttcacga gcggggcgat
     2941 cctgaagacg gggatctggg gcgagaccct ggagatccag cggttggggg cgagcggcct
     3001 gcgcaaggtc cgggcggtcg ccgggatcca tgtctgggag cagttcctca acgtccggag
     3061 cggccggatc cccaatccga gcccgcccga ggtcggcctc cggatggcgc ggctgtggga
     3121 cgcgatccgg gcgtccgcgg cgatggccgg cgccgtcgtc cggctgccgg cgagcgagcc
     3181 ggtcctgacg gggaccgcgg cggccggccg atgacccgcg tcacggtctg gagcgagcac
     3241 cgccaggagc ggtccgaccc gccggtggcg gcgatctatc ccgacgggat ccacggcgcg
     3301 atcgccgcgg ggctccgcga cgcgggcttc gaggtccgca cggccaccct cgacgagccg
     3361 gagcacggcc tgaccgaggc ggtcctcgcc gcaaccgacg tcctgacgtg gtggggccac
     3421 gtcgcgcacg acgaggtcga cgacgcgatc gtcgaccggg tccagcgccg ggtcctcgac
     3481 gggatgggcc tgatcgtcct ccactccggg caccactcga agatcttccg ccggttgatg
     3541 ggcacgaccg ccgacctgaa gtggcgcgag gcgggcgagc gcgagcggat ctgggtcgtc
     3601 gaccggtcgc atccgatcgc cgacggcctc ggggattcgt tcgtcctcga cgaggaggag
     3661 atgtacgggg aggcgttcga cgtgcccgcc ccggaccagc tcgtcctcat cagctggttc
     3721 cagggcggcg aggtgttccg gagcggctgc tgctggagcc ggggacgcgg tcgggtcttc
     3781 tacttccggc ccggccacga gacgtatccg acgtacttcc agccgatggt tcgacgggtg
     3841 atcgcgaacg ccgtgagctg ggccgtcggt ccgcgcgggg ccccgatcgt cgtcgccaac
     3901 caggacgcgc cggaggggct ggaacgaggc tgacgggcgc ggtccgtccc tcagccgagg
     3961 accaccacct cgtcgccgag cgcgatgtcg ccgtcggtca ggatcctcgc ccgcagtccg
     4021 gcccggtgga cgagcgccgt caacaccggc ttcccgagaa ggtccgtcag gtactggcac
     4081 ggctcgcaca ggcgcgttcc ctcgcacgcg acagcgccga tccggaatcg tcgtccgacg
     4141 agctcgttga ggcggatccc gcgggtcgtc acgttccgcc gcgtcgcgcc cggctccagc
     4201 gtgatcccgg acccgacggc aagcgcctcg atctcctcgg cctcgatcag ggtgacgtgc
     4261 cggtcgacct tcgtgtcgcc cgagtagtga cccgctcccg tcgcgtagcg gtcgccttcg
     4321 aggccgacac cggcgatcgc ccgcacccgc tcgatcgaat gcatcggctc gccgccgccc
     4381 gatgtcacgt ggatcgcctc gacggcgccg cgtccgggtc cggaacgatc cgcctcgagc
     4441 gtcgccggct cgggacgggg tggcgaagcg ggcatcggcg acattgtcgc gatcgagtcg
     4501 acgttcgtcc acggcgccgc gctggcggac cgggcattct ggcagcgtga ccgacggaac
     4561 ccacccgaac cgcgcccatg acgacggtct ctggtcgccc ggccggcggg cgctgacgat
     4621 cgggctcgtc ctgaacgtca cgatcgtcgc gtcggaggcc cttgcggtcg ggacgatcct
     4681 gccgatcgtc gcgaaggacc tcggtggcat cgacctctac ggctgggtct tctccgggtt
     4741 cttcctcggc aatctcctcg gcatcgtcgt cgccgggatc ctgatcgacc gcggcgggct
     4801 gacccgaccg ttcctcatgg ggatcggcct gttcgcgatc gggctgctgg tcggcggcct
     4861 ggcgccctcg atggagatcc tcgtgatcgc ccgcctcatc cagggcttcg gcgccggggc
     4921 gatccccgcg gtcgcctacg tctcgatcgg cagggcgatg cccgcgcacc tccgtccgcg
     4981 gatgttcgcg acgctctcga ccgcgtggat cctgcccggc gtgatcgggc cggtcgcggc
     5041 atcggcggtg accggcctct tgaactggcg gatcgtgttc ctgggccttc tgccgttgat
     5101 cgcgatcgcc gcgacgctga ccctgcccgc gatccgcgcc gtggccgcgg ctccggccgc
     5161 ggccgccggg accccggcat cggcgcggat cgcggccggt cgcgtccgcc tgccgctcgc
     5221 cttcgtcctc gtcgtcggtg cgggcctggt cgtctcgggc ctgtcgaacg tgtcgcccat
     5281 cccgctcgtc ctgatcgtca tcgggatcgc gatcggcgcc ccggccttcg cccgcctcac
     5341 gccacgcggc acgatccggg ccgcaactgg tctgccgacc gcgatcctgc tccgcggttt
     5401 cctgacgttc gccttcttct gcgccgatgc ctacgtaccg ctggtgatcc agggatggcg
     5461 cggcgactcg gcgtacatgg ccgggttcac ctacatggcg gcgacgcttg cctggacggg
     5521 cggctcgtgg gtcaacgctc atcacatcgg gaggctcggg ccgggtcggt tcctgcgcgt
     5581 cggcttcgtc gtcgtgctgg tcgggatcgt cgggttcctc gcggtcctcc tgccgtgggt
     5641 cccgccggcc gccgggatcg tgatctgggg tgtcgccggc ttcgggatgg gcctcgccta
     5701 ctcgacgccg tcgctcatcg tcctcagtga ggcaccggcc ggcgagcagg gggcggccac
     5761 ggccggcctc cagctgtccg acgtcctcgg agcgtccctc gggacgggga tcggcggagc
     5821 cctgatcgcc ttcggatcgc gcgccggcag ccccggctgg gagggcctgg cggcgacgtt
     5881 cgcggtcgcc ggggtggtcg ccctgatcgg cctggcgctg acgacccgcc tcggggcgcg
     5941 gaccgcagcc gggttgccgc cgacccatct cgggaccgca acggcctggg cgacgagcga
     6001 gacggggcgc gacccggccg tgcggtagac ttgcgacccg tccgccgcac cggaacccga
     6061 cctgcggacc gcccagctgg aggaaccctc gaccgatgtc cgccgaggag cttcgcgcaa
     6121 agatccgcga gatcccggac tttcccaagc ccggcatcct cttctacgac atcacgaccc
     6181 tgctgaagga tcgcgctgcc tacaaggagg cgatcgacct gatgctcgcg ccctaccagg
     6241 gcgagaagat cgacgtcgtc gtcggcatgg agtcgcgcgg gttcatcttc agctcggcca
     6301 tggcctacca gttgaaggcc gggctcgtgc cggttcgcaa gctgggcaag ctgccggccg
     6361 agacgatcac cgtcgagtac gcgctcgaat acgggtccaa cacgctcgag attcatcgcg
     6421 acgcgatcga gcccggccag aaggtcctga tcgtcgatga cctcctcgcc accggcggca
     6481 ccgtcaaggg cacgatcgag ctgatcgagc ggctgaaggg tgacgtcatc gggcttgcgt
     6541 tcctcgtcga gctcgacttc ctcaagggcc gtgaccggct cgacggccgg cgggtcacct
     6601 ccgtcatcaa gtactgagag cccgggccgg cccagtcgcg tcgcgacatc ccgacccgat
     6661 tgccagcgag tccgccttgg ccgacgcgat ccgcgactcc gaccacgacc cgcccgccgc
     6721 tccggatgat gacggctcgg ccgtcgtcga cccggcaggc gcggtcgatc cggcggccgt
     6781 ccacatctcg accggtgccg tcgagccggc cgccgacggc gacccgacga acctcgcccg
     6841 acggcggttc tttcgccagt tcgcaggcga gctgatccag accgccgcga cggtcgccgg
     6901 cgcggcgcag gccctccagc gggcatcggc ggaggcggcc ggcgccatcc tcgatcccgt
     6961 agcggcggcc gcgcggcttg aggccgcccc ggtctcggtc ggcacgaccg cctccggcct
     7021 gactgaccgg gggtcggcgg cggattcgcc cggcggcccg accggcttcc ggacgccgtt
     7081 ccgcgaggac gacggcgtgc tccacctgat cgatcagcgg aagctcccgg accagctcgt
     7141 cgaggtcgag gtccgaaatg cccccgaggc cgcgaccgcg atccgccaga tggtcgttcg
     7201 tggcgctccc gcgatcggcc aggtcgccgc gatcggcctg gccctgtcgg ccgaccgctc
     7261 gaagaccacc cagccattcg cccggcgggc gatcctccgc ggcggcgcgg cggccctccg
     7321 cgccgcccgg cccacggcgg tcaacctcgg ctgggcggtc gagcggatga tggccgcgtt
     7381 cgaggcggtc ggcgatctga gcgaggacgg tccggccgtg gcggccgcgt tgcgggccga
     7441 ggccgacgcg atcgtcagcg aggcgaccac cgaccatggc cggctggccg agttcggcct
     7501 cgcggtgctg ccggtgaagg aattcgggcc gctccggatc ctgacccact gcaacaccgg
     7561 gccgctcgcc tgcggccagt tcggaacggc gctcgggatc gtccaggccg ctcaccacgc
     7621 cgggcgcgag gtcaccgtct gggtcgacga gacgcggccg tacctccagg gcgcccgcct
     7681 caccgcttgg gagctggccc aggccggcgt gccccacacc ctgatcccgg acgttgccgc
     7741 ggggttcctg atgagccgcg gcgaggtcga cgtcatcctg gtcggagccg accgggtggc
     7801 ggccaacggc gacacggcca acaaggtcgg gacgtattcg ctcgcggtcc tggcggcccg
     7861 ccatcggatc ccgttctatg tctgcgcgcc gacgaactcg gtcgacctga ccacgccgga
     7921 cggcgactcg atcgagatcg aggagcgctc cgccgacgag gtgctgctga tccgcggcgt
     7981 cgcgatcgcg ccgcccggga ccgccgtgcg caatccgtcc ttcgacgtga cgccggccga
     8041 gttgatcacc gggatcgtca ccgaagaggg cgtcgtgacc gcgccgtacg aggccgggct
     8101 cggtgccgcg atcgcggcgg cgaagcttcg ctgggcgccg cggcccctga tcccgccaac
     8161 gccgcgtccg cccgtcgaac gggccgacga cggagccacg gccgccggcc ccggaatcac
     8221 caccggagtt cccgactgat ggcgagcgtc gccgcgacag gtcgtcgggc ccgcggcttc
     8281 acgatccgat cggtcaccga ccgcggccag ttgcgggcct tcctcgagac ggaccggctc
     8341 ctcgccgctt acgcgatctg cgacctcgag gacaaggaat tcggcaagac ccgctggggc
     8401 ggcgccttcg agggcgaccg gctcgtcgcc cttgccctgg agtactcggg cctgaccccg
     8461 cagccgctct tcgtgatggg ccgggccgag ggggtcgagg cgatcctccg cgacgtcgtc
     8521 cggccgcggg cggcctacgt cgccgtcctg cctgaagcgt tgcccgccgt ggagaccgtc
     8581 taccgcctcg accccggtcc gcagatggtc cggatgtggg tcgatcgcgg ccggttccgc
     8641 ccgtatccgg cggatgtccg acgcctcctg cccgccgaca tcggcgagct caaccggctc
     8701 taccagctcg gctttgcctc gtggcttccg tcgtcctcga tcgccgacgg ggtctacttc
     8761 gggatccgcg tcggtggccg gctggtggcg gccgccggga cccatgtcat cagccgcgag
     8821 gcccgcctcg ccgtcgtcgg caacgtcctg acccatgtcg actaccgtgg ccgtggcttc
     8881 gcgaccgcgg tcaccggtgc ggtgaccgcc gagctcctcc ggacgtcgga ccaggtcgtc
     8941 ctcaacgtcc gttccgacaa tccgcccgcg ctccaggcat accggcgcct cggctacacc
     9001 gagcacgtcc ggttcgagga gcgcctcgcc catcgcctcg gttcgccgtg gcccgacttc
     9061 acggcgccga tccgccgcct cttcagccgc agggagaact gatcccgatg accgataccg
     9121 cagctccgcc tcagggcgct ccgccccccg ccacggccgg cctgccggcc cacgacgtga
     9181 ccgacctcgg gctcgcgtcg gagggcgtcc gccggatcga ctgggccgag cgggagatgc
     9241 ccgtcctgcg cctgatccgc gagcgcttcg agcgcgagca accgctcgcc gggatccgga
     9301 tcggagcgtg cctccacgtc acgaccgaga ccgccaacct gatgcggacc ctcaaggccg
     9361 gcggggccga ggtgttcctc gccgccagca acccgctctc gaccaaggac gacgtcgccg
     9421 cggctctcgt ggccgagtac ggcattgcga ccttcgcccg ccgcggcgag gatggggaca
     9481 cgtactacgc gcacctcaac gcgatcgccg acacgaagcc gcagatcacg atggacgacg
     9541 gctgcgatct cgtggctctc ctgcaccggg agcgcccggc ccaggtcaag gaggtcctcg
     9601 ccgggaccga ggagacgacg accggcgtca tccggctcaa ggcgatggcc gccgacaagg
     9661 ccctcggctt cccggtggtc gcggtcaacg aggcccagac caagcacctg ttcgacaacc
     9721 gctacgggac cggccagagc acgatcgacg ggatcctccg ggcgacgaac atcctcctcg
     9781 ccggccggaa tgtcgtcgtc gccggctacg gttgggtcgg caagggcatc gcgtcgcgga
     9841 tgagcgggat gggcgcgcat gtcgcggtgg tcgaggtcga tccggtgcgc gccctcgagg
     9901 cgctgatgga cggcttccgg gtgatgacgg tcggcgaggc cgccccatgg ggccagctgt
     9961 tcgtgaccgc gaccggcaac gtcaacgtct tccggcgcga gcacttcgcc gcgatgcagg
    10021 agggcgccgt catggccaat tcgggccact tcgacgccga gctggatctc aaggcgctgc
    10081 gcgagatggc cgagggccac gtccgcgacg cgcgcgagaa cgtccaggag ttcgacatcg
    10141 gcggccgcaa gatcaacctg atcgcggaag gccggctggt caacctcggc gcggccgagg
    10201 gccacccggc cgcggtgatg gacatgagct tcgccaacca ggcgttgtcg gccgaatacg
    10261 tcgtccagca ccatgccgag ctgaccccgc aggtgtacat cgtgcccgag gcgatcgacg
    10321 ctgaagtcgc ccggctcaag ctggccgcga tggggatcac cctcgacgac atgaccgccg
    10381 aacagaagga gtacgtctcc tcgtggcagc acggcacctg agtcggtgag cgacgtgcat
    10441 cgcgcggcgc cctacgggtc atgggtctcg cccttcccga tcgagctgct gagcaggggg
    10501 gccgtcacct ttggcgagat cggtgcctcc gggggcgcct gctggtggct cgagggtcgg
    10561 gccgaggagg gcggccgcca ggtcctcgtc cggcgcgagg ccgacgggac tctgacccgc
    10621 ctgacgccgg agggcttcaa cgcccgctgt cgcgtccacg agtacggcgg tggggcgtac
    10681 ctgatcgacg gcgacctggt cgtcgtctcc gacttcacca cgggtcggct ccagcgggtc
    10741 accgcgcccg gtgtgctcga accgctcacg ccggccggga aggcgtggcg attcgccgac
    10801 ctgagcctcg atcgtgggcg caaccggatc ctcgcgatcc gggaagacca cgagccggcc
    10861 acgatcgccc gtcatggcga ggccgagaac gctgtcgtcg cgatcgacct cgccgacggc
    10921 tcggtcacgg tcctcgccga gggcgccgac ttcttctcgt cgccgcggct ctcgccggac
    10981 ggcagccggc tcgccttcct gcgctggaac cacccgaacc tgccgtggga cggcaccgag
    11041 ctctgccttg ccgatctcgg cgccgatggg tgcccggcga aggagcgcgt cgtcgccggc
    11101 agccccgcgg actggatcgc ccagccacgc tggtcgccgg ccggagtcct ccatttcgtc
    11161 gccgagcccg acggctggat gaacctgatg cgcctcggcg cgggcgatcg ggtcgagcga
    11221 gcgagcgagc cgatcgaggc cgagttcgcg ttcccggatt gggtgttcag cctcgcgaac
    11281 tacgcgatcg cggcggacgg cacgatcatc ggcgccggcc ggagcggcgg gcgcgatcgg
    11341 ctgtaccgga tcggcccgga cggatcggcg acgacgttcg acctgccgtt caacgagttc
    11401 ggctatgtcg cgatcgatgg ctcgaccgcc gtcttccggg cggccgccgc cgatcggccg
    11461 tgggccatcg tcgagctcga cctggcgacc gccgcgacca ccgagctgag gcgatccatc
    11521 gagctcgtgg tcgatcccgc cgacgcctcc ctcggtgagc tcgtcgagtt cccgaccgag
    11581 ggggaccgga ccgcgttcgg gatcctctat cggccgaaac accagacctg cagcggtccg
    11641 gtcgatgccc ggccgccgct gatcgtgacc agccacggcg gcccgacggc cgaggcctcg
    11701 accgcgttcg ccgtcctgat ccagttgttc acgagccgcg gtctcgccgt cctcgacgtc
    11761 gactacggcg gctcgacggg ctacggcatg gcctatcgca agcggctcga aggggagtgg
    11821 ggggtcgtcg atctcgccga ctgcgtcgcg ggcgcccgct ggctggctgc cgagggcatc
    11881 gtcgacggtc agcaactcgc gatccgcggc ggcagtgcca gcggctacac gaccctgtgc
    11941 gccgtgacct tcagcgacgt gttctccgcg gggacgagct acttcgggat cggcgacctc
    12001 gagacgttcg ccaggacgac ccacaagttc gagtcgcgct acctcgaccg gctgatcggg
    12061 ccgtaccccg agcggaagga tctctatcac gaccgctcgc cgctgaactt cgccgaccgg
    12121 atcgcctgcc ctgtcctgat catgcagggc gcggaggatc ggatcgtccc gcccgcccag
    12181 gccgagcaga tcgtcgacgt gctctgggag cggcggctgc cccacgccta catcctcttc
    12241 cccggcgagg atcacgggtt ccgcagcgcc gccaacatca tccgctcgtt cgaggccgaa
    12301 ctgtcgttct acggccaggt cttcgggttc acgccggccg acgcgatcga gccgattgcc
    12361 gtcgagttcc tcgatggcgg gccggccacg gcggatgggg ccggcccgcg acgcgcgacg
    12421 ccggcctgat tcgtcgcgcg gcggatcatg gccgcagctc caccgcgatg atcgatgccg
    12481 gtccgttcat cggaagcgtg atcgccgtga tccgggtccg atcagccagg tcaacgagga
    12541 t
//
LOCUS       DATAIW010000368         6840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_173252, whole genome shotgun sequence.
ACCESSION   DATAIW010000368 DATAIW010000000
VERSION     DATAIW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173252"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2209)
                     /locus_tag="VHM48_11075"
     CDS             complement(<1..2209)
                     /locus_tag="VHM48_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2755999.1"
                     /translation="MDARSIGLLEFPLVRERLAEKTSFAPSRRLAEALLPESDPVLVA
                     RGLDETDQARSLLSERPGVGIGAARDIGPAVERAARGGRLDAAQFLELADTLDATPRL
                     QTSLADDRRPLLHAIARELHALPAIRSTLARSFDPAGELLDTASPRLGGLRAAVRVAY
                     DRLRRRLDSLVGSELGGALQDPIITLRNGRYVVPVKAEARAKVKGIVHDASGSGATLF
                     IEPLVAVELGNAWREAQIAAQEEIDRILDELSAFVAANAIQLRETLGALARFDFWAAK
                     AGLAADMDGTRAETVPRPEVILLGARHPGLTGRVVPTDIRLGDGYTALVVTGPNTGGK
                     TVTLRTLGLLSLMHQAGLHVPAETGSRLPIFRDVFADIGDEQSIAQSLSTFSGHLRSI
                     TRIVEAAGPGTLVLLDELGAGTDPTEGSALAQALLDHFIKAGVLVAATTHYAELKAYA
                     HTTAGARNASVEFDLETLSPTYKLTIGLPGGSQAFAIAERLGLDAGIVADARSRLSEN
                     QRAFEETLARIRATEGETSEALDRARAAESRATDALRTADEERRRARRERDEAVSAAR
                     VEAERLVDDLRDEVRATRQALERETVTAPSLDAAVARAEAQLARLTDEPALEPEPVAV
                     APEAPHVWQLGDHARSRSGGWEGRIAALEKSGTRATLEAGEMRISVATDDLDLIATPP
                     PIDHDRTIGGNARITTARVAAGRDPRTGRWSAPRTVTPGNGGGVGVGSMRLDRA"
     gene            complement(2213..2926)
                     /locus_tag="VHM48_11080"
     CDS             complement(2213..2926)
                     /locus_tag="VHM48_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2756000.1"
                     /translation="MTRRPERDRLLELPTLAIAAQLLGWRLVRAADDAGGLRVGRVVE
                     IEAYIGEDDRACHARFGRTARNDVMYGPPGRAYVYLVYGMHDCLNIVTEPAGSPAALL
                     VRAVEPLEGADRMRASRARRGAARRRSAPAPGAIDGRSDRPTLATHRLAAGPGLVCAA
                     FDLDRTLTGHDLFDPAAAIRLEPPPAAEAAPIVVSGPRVGVAYAGIPWTALPWRFAIA
                     ANPAVSRPPIEPASGRRPR"
     gene            complement(2949..3545)
                     /locus_tag="VHM48_11085"
     CDS             complement(2949..3545)
                     /locus_tag="VHM48_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX2756001.1"
                     /translation="MNIGEFIANLNRFDLVVVLLVFGMFVLGFVQGTIRRILGLASML
                     FSFLFAANLRDPLGNYLTQNWYGFPDGYAVMLGFGIVFVAATVAFTLVIQGFYHKQNL
                     FQKATFADELIAGALGIVQALFLVGCIIIILDTFFRVPGIPKSDTEFGWIRSLFDEMN
                     KSGTADLFRSRLIPGFIAVFGLLIPTDIQKFFVTVPPG"
     gene            complement(3635..6166)
                     /gene="pheT"
                     /locus_tag="VHM48_11090"
     CDS             complement(3635..6166)
                     /gene="pheT"
                     /locus_tag="VHM48_11090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2756002.1"
                     /translation="MRVPLSWLRDYVDVDLAPEALAERLTLLGMEVKGIERWGADWRN
                     VVVGELLSVEKHPRADRLSLTRVRVGDGEPLDIVCGATNIAAGQRVPVALPGAVLPGE
                     RRIERTEKMGVVSNGMLCSGDELNLTGDADGILILATDAPIGMALTELYGDVVLDVDV
                     KPNRGDGLSLVGLAREVGAITGAPLRWPECDPAEDERDVANLLAVEVRDSDWCPRFVG
                     RWVDGLTVGPSPDRVQMRLRAAGQRPISNVVDVSNYVMLELGKPIHAYDADHVAERDG
                     RRQLVVRRAAAGERLETLDHVTRELDPDTLVIADDRAALGIAGIMGGADSEVADRTSR
                     VIVESAIFDPIVIRRTGQRYGLRSEASLRFEKGQELRLARIGADRAARLVAEWAGGSV
                     ARGRVDSAAMEPEPARVAFRPARVNRLLGTTFSTAEQVALLARVGVATDAGSSDAGPT
                     GGRARHDIVVAGAPKALAVDAGSDEVVVAIVPSWRRDIAIEADVSEEVARIGGYDAIP
                     AILPHTPMPAWRPSPLELRDRVRETLAGAGLTETVSHALISPRLATTFTWAAELPPVA
                     GGTPALGRTIHVTNPLSADHSVLRPALVGSLVEIVSTNLRRGREDVAIFEIGKGYGRD
                     GDATREWWRLGLAMAGAFEEPSWNRPRREADLDDAKGVIELVCHRLGFDAPVWTVLDG
                     EPLLHPGRAARVSATRNGAIALGGVLGELHPAAADTVDLRGARLVVAELEIAGLAGGR
                     PADVHVAAPPRHPAAQRDIAIVVSETVAAATIAAAIRDATGSQLVSVRLFDIYRGTPL
                     GPGEKSLAWRLVFQADERTLTEPEIEASVGRITSAVARAGGRIRT"
     gene            complement(6171..>6840)
                     /locus_tag="VHM48_11095"
     CDS             complement(6171..>6840)
                     /locus_tag="VHM48_11095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2756003.1"
                     /translation="QFGFVTHEGPEVEDDLTNFQMLNIAPDHPARDLWDTLYVDIDGQ
                     LLRTHTSPGQIRVMTSTAPPIRALLPGRCYRYEAIDASHASEFFQVEGLMVDEGTTMG
                     DLKGLLDQFAKALYGADKRTRFRPGYYPFTEPSVAFDVECLVCGGSGCPACSRTGWMT
                     ILGAGMVHPVVLQYGGLDPERYQGFAFGMGVERIANLRHSVGDLRLYMENDLRFLDQF
                     VRAG"
BASE COUNT          977 a         2395 c         2543 g          925 t
ORIGIN      
        1 gcgcccggtc gagccgcatc gatccgactc cgaccccgcc gccgttcccc ggagtcaccg
       61 ttcgcggggc ggaccagcgc ccggttcggg gatcccgccc cgcggcgacg cgtgcggtcg
      121 tgatccgggc gttcccgccg atcgtccggt cgtggtcgat cggcggcggg gtcgcgatca
      181 ggtcgagatc gtcggtcgcc acgctgatcc gcatctcgcc ggcttcgagc gtggcccgcg
      241 tgccgctctt ctcgagcgcc gcgatccgac cctcccagcc cccgctccgg ctgcgcgcgt
      301 ggtcgccgag ctgccagaca tggggggcct ccggggcgac ggcgacgggc tccggctcca
      361 gcgccggctc gtccgtgagc cgggcaagct gcgcctccgc ccgggccacc gccgcatcga
      421 gcgatggtgc ggtgaccgtt tcccgttcga gggcctggcg cgtcgctcgg acctcgtcgc
      481 ggaggtcgtc gacgagccgc tcggcctcga cccgggctgc gctgacggcc tcatcgcgtt
      541 cgcggcgcgc gcggcggcgt tcctcgtcgg cggtccgcag ggcgtcggtc gcccgcgatt
      601 cggcggctcg cgcacgatcg agcgcctcgc tggtctcgcc ctcggttgcg cggatccggg
      661 cgagcgtctc ttcgaacgca cgctggttct ccgacaggcg cgagcgggcg tcggccacga
      721 tcccggcgtc gagcccgagt cgctcggcga tcgcgaaggc ctggctgccg ccgggcaacc
      781 cgatcgtcaa cttgtaggtt ggcgacagcg tctcgagatc gaactcgacc gaggcgttcc
      841 gggcgccggc ggtcgtatga gcgtaggcct tgagctcggc atagtgggtc gtcgccgcga
      901 cgaggacgcc cgccttgatg aaatggtcga gcagggcctg ggccagcgcc gagccctcgg
      961 tcggatccgt cccggcgccg agctcgtcga gcagcaccag ggtgccgggt ccggccgcct
     1021 cgacgatccg ggtgatcgag cggagatgac ccgagaacgt cgagaggctc tgggcgatcg
     1081 actgctcgtc gccgatgtcg gcgaagacgt cgcggaagat cggcagacgc gagccagtct
     1141 cggccgggac gtggaggccg gcctggtgca tcaggctgag gagcccgagg gtccgcaggg
     1201 tgacggtctt gccgccggtg ttcgggccgg tcaccacgag cgccgtgtag ccgtcgccga
     1261 ggcggatatc cgtcgggacg acccgaccgg tgaggcccgg gtggcgggcg ccgagcagga
     1321 tgacctccgg ccgcggcacg gtctctgccc gcgtcccgtc catgtcggcc gcgagcccgg
     1381 ctttggccgc ccagaaatcg aaccgggcga gggcgccgag cgtttcgcgg agctggatgg
     1441 cgttggccgc gacgaacgcg gagagctcgt cgaggatccg gtcgatctcc tcctgggccg
     1501 cgatctgggc ttcgcgccag gcattcccga gctcgaccgc cacgagcggc tcgatgaaca
     1561 gcgtggcgcc gctgccggac gcgtcgtgga cgatcccctt gaccttcgcc cgggcctccg
     1621 ccttgaccgg cacgacgtag cggccgttgc ggagggtgat gatcgggtcc tggagagcgc
     1681 cgccgagctc cgagccgacc agagaatcca accgccgccg gagccggtcg taggcgaccc
     1741 ggacggccgc tcgcaggcca ccgagccggg gcgaggccgt gtcgagcagc tcaccggccg
     1801 gatcgaagct gcgggccagc gtcgagcgga tcgccggcag ggcgtgaagc tcgcgggcga
     1861 tggcgtgcag gagcggccgg cggtcgtcgg cgagcgaggt ctggaggcgg ggggtcgcgt
     1921 cgagggtatc ggccagctcg aggaactggg cagcgtcgag acgtccgccg cgcgctgccc
     1981 gttcgaccgc cggcccgatg tcgcgggccg ctccgatccc gacgcccggc cgctccgaga
     2041 ggagcgagcg ggcctggtcg gtttcatcga ggccgcgggc cacgaggacg gggtcgctct
     2101 ccggaaggag cgcctccgcc agccggcgcg atggcgcgaa ggacgtcttc tcggcgaggc
     2161 gctcgcggac gagcggaaac tcgaggaggc cgatcgagcg ggcgtccatc ggtcagcggg
     2221 ggcgtcggcc ggacgcgggc tcgatcggcg ggcgtgacac ggccgggttg gcggcgatcg
     2281 cgaaccgcca cggcagcgcc gtccacggga tgccggcgta ggcgacgccg acccgcggtc
     2341 cgctgaccac gatcggcgcc gcctcggcgg cgggcggcgg ttcgaggcgg atggccgcgg
     2401 ccgggtcgaa gaggtcgtgg ccagtcagcg tccggtcgag gtcgaaggcc gcgcagacga
     2461 gccccggtcc ggcggccagc cggtgcgtgg ccagcgtcgg tcggtccgac cgtccgtcga
     2521 tcgccccggg cgccggcgca ctccgtcggc gcgctgcgcc gcgacgagct cgggaggcgc
     2581 gcatgcggtc cgcgccctcg agcggctcga cggcccggac gagcagggcg gcaggcgatc
     2641 cggccggttc ggtgacgatg ttcaaacagt cgtgcatgcc gtagacaagg tacacgtagg
     2701 cccggcccgg ggggccgtac atgacgtcgt tgcgcgccgt ccgcccgaac cgcgcatggc
     2761 aggcgcggtc gtcctcgccg atgtacgcct cgatctcgac gacccgtccg acgcggagcc
     2821 cgcccgcatc gtcggcggcg cggacgaggc gccagcccag cagctgcgcc gcgatcgcga
     2881 gggtcggcag ctcgaggagg cgatcgcgtt ccggtcgccg ggtcatcgcg tcccggaccg
     2941 acgccggatc agcccggcgg gaccgtgacg aagaacttct ggatgtcggt cgggatcaac
     3001 agcccgaaga cggcgatgaa gcccgggatc aggcgtgacc ggaacaggtc cgcggtgccg
     3061 gacttgttca tctcgtcgaa caggctgcgg atccagccga actcggtgtc ggacttcggg
     3121 atgccgggga cccggaagaa cgtgtcgagg atgatgatga tgcagccgac caggaacaac
     3181 gcctggacga tcccgagcgc gccggcgatg agctcgtcgg cgaacgtcgc cttctggaac
     3241 aggttctgct tgtggtagaa gccctggatc acgagggtga acgcgacggt cgcggcgacg
     3301 aacacgatcc cgaagccgag catgaccgcg tacccgtcgg gaaagccgta ccagttctgg
     3361 gtgaggtagt tgccgagggg gtcgcgcagg ttggcggcga acaggaatga gaagagcatc
     3421 gacgcgagcc cgagaatccg cctgatcgtg ccctggacga acccgagcac gaacatcccg
     3481 aagacgagca ggacgaccac caggtcgaac cggttgaggt tggcgatgaa ttccccgatg
     3541 ttcacggacg ctccctctga acgagacccc cgaccggctc acgtcgcctg agagtcacag
     3601 cgtagcagcg cggaatcggc aggggcaaca ggactcaggt cctgatccgg ccgccggccc
     3661 gagcgaccgc ggacgtgatc cggccaaccg acgcctcgat ctcgggctcg gtcagggtcc
     3721 gctcatcggc ctggaagacg agccgccagg caaggctctt ctcgcccggg ccgaggggcg
     3781 tcccgcggta gatgtcgaac aatcggaccg acacgagctg ggacccggtc gcgtcgcgga
     3841 tcgccgcggc gatggtggcc gcagcgaccg tctccgacac gacgatcgcg atgtcgcgct
     3901 gggccgccgg gtgccggggc ggggcggcca catggacgtc ggccggccgg ccaccggcca
     3961 gcccggcgat ctcgagctcg gcgacgacca ggcgggcacc gcgcaggtcg acggtgtcgg
     4021 cggctgccgg atgcagctcg ccgagcaccc cgccaagcgc gatggccccg ttgcgggtcg
     4081 cagacacccg ggcggcgcgg cccggatgga gcagcggctc accgtccagg acggtccaga
     4141 cgggggcgtc gaatcccagc cggtggcaga cgagctcgat gacgcctttc gcgtcgtcga
     4201 gatccgcttc ccgccgtggt cggttccagg acggttcctc gaacgcgccc gccatcgcga
     4261 ggccgaggcg ccaccactcg cgggtcgcgt ccccatcgcg accgtagccc ttgccgatct
     4321 cgaagatcgc gacgtcctcc cggccgcgtc gcaggttcgt cgacacgatc tcgacgaggc
     4381 tgccgacgag cgcggggcgg agcaccgaat ggtcggccga cagcggattc gtgacgtgga
     4441 tcgtccgacc gagggcaggc gttccaccgg cgaccggcgg cagttcggcg gcccaggtga
     4501 acgtggtcgc aagtctcggc gagatgagcg catggctgac ggtctcggtc aggccggcgc
     4561 cggcgagcgt ctcgcggacg cgatctcgaa gctcgagagg cgagggccgc caggcgggca
     4621 tcggggtgtg cggcaggatc gccggaatcg cgtcataccc gccgatccgg gcgacctcct
     4681 cgctgacatc ggcctcgatc gcgatgtcgc gccgccagct cgggacgatc gcgacgacga
     4741 cctcgtcgga cccggcatcg acggccagtg ccttcggggc acccgccacc acgatgtcgt
     4801 ggcgcgcccg accgccggtc ggcccggcat cagacgatcc ggcgtcggtc gcgaccccga
     4861 cccgcgccag gagggccact tgctccgctg tcgagaatgt cgtcccgagg agccggttga
     4921 cgcgcgccgg gcggaacgcc actcgggccg gctccggctc catggcggcg ctgtcgacgc
     4981 gcccgcgagc gaccgatccg ccggcccact cggcgaccag ccgggccgcc cggtcggccc
     5041 cgatcctggc gaggcggagc tcctggccct tctcgaagcg gaggctggcc tcggagcgaa
     5101 ggccgtagcg ctggccggtc cggcggatga cgatcgggtc gaagatcgcc gactcgacga
     5161 tgacccggct cgttcgatcc gccacctcgg agtccgcgcc gcccatgatc ccggcgatcc
     5221 cgagcgcggc ccggtcgtcg gcgatgacga gggtgtctgg atcgagctcg cgcgtcacgt
     5281 gatcgagcgt ctcgaggcgc tcgccggcgg ccgctcgccg gacgacgagc tggcgccgcc
     5341 catcgcgctc ggccacgtga tcggcgtcgt aggcgtggat cggcttgccg agctcgagca
     5401 tgacgtagtt gctgacgtcg accacgttgc tgatcgggcg ctggccggcg gcccgcaggc
     5461 gcatctggac acggtccggc gacggcccga cggtcagccc gtcgacccag cggccgacga
     5521 agcgcgggca ccagtcgctg tcgcggacct cgacggccag caggttcgcg acgtcgcgct
     5581 cgtcctcggc cgggtcgcac tccggccagc ggagcggtgc gccggtgatc gcgccgacct
     5641 cacgggcaag gccgaccagc gagaggccat cgccgcggtt cggcttgacg tcaacgtcga
     5701 ggacgacgtc gccgtacagc tcggtcagtg ccatcccgat cggggcgtcg gtcgcgagga
     5761 tcaggatccc gtcggcgtcg cccgtcaggt tcagctcgtc gcccgagcag agcatcccgt
     5821 tgctgacgac gcccatcttc tcggtccgct cgatccgccg ctcgccgggc agcaccgcgc
     5881 cgggcagggc aaccgggacg cgctgcccgg ccgcgatatt cgtggcgccg cagacgatgt
     5941 cgagcggctc gccgtcaccg acccggaccc gggtcagcga caggcggtcg gcacgcggat
     6001 gcttctcgac gctgaggagc tcgccgacga cgacgttccg ccaatcggcg ccccaccgct
     6061 cgatcccctt gacctccatc ccgagcaggg tcagccgctc cgcgagcgcc tccggcgcga
     6121 gatcgacatc gacgtagtcg cggagccagg acaggggcac tctcatggcc ctatcccgcc
     6181 cgcacgaact ggtcgagaaa gcggaggtcg ttctccatgt acagccgcag gtcgccgacc
     6241 gagtggcgca ggttggcgat ccgttcgacg cccatcccga acgcgaaccc ctggtagcgc
     6301 tccgggtcga ggccgccgta ctggagcacg accggatgga ccatcccggc gccgaggatc
     6361 gtcatccagc cggtccgcga gcaggccggg cagcccgagc cgccgcagac gaggcactcg
     6421 acgtcgaacg ccaccgacgg ctcggtgaac gggtagtagc cgggccggaa gcgggtccgc
     6481 ttgtcggcgc cgtagagggc cttcgcgaac tggtcgagga ggcccttgag gtcgcccatc
     6541 gtcgtgccct cgtcgaccat caggccctcg acctggaaga actcggaggc gtggctcgcg
     6601 tcgatcgcct cgtaccggta gcagcggccc ggcaggaggg cccgaatcgg cggggccgtg
     6661 gacgtcatca cccggatctg gccgggcgag gtgtgggtcc gcaggagctg gccgtcgatg
     6721 tcgacgtaga gcgtgtccca caggtcgcgc gccgggtggt ccggcgcgat gttcagcatc
     6781 tggaagttcg tgaggtcgtc ctcgacctcg ggtccctcgt gggtgacgaa cccgaattgg
//
LOCUS       DATAIW010000369         5015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_113632, whole genome shotgun sequence.
ACCESSION   DATAIW010000369 DATAIW010000000
VERSION     DATAIW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113632"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VHM48_11100"
     CDS             complement(<1..654)
                     /locus_tag="VHM48_11100"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2756004.1"
                     /translation="MVGPAARLPLRRSAVPLDGRRVHLRPAEPADAPAFTTILSDPTV
                     HPWWQAADPAADARQLADRADHVAVWAIEEAGVIVGVIQAHEETDPRYRHAGIDIVLA
                     GSAQDRGLGSDAVRTVARWLIDVRRHHRLTIDPAAANERAIRAYRRVGFRPVGIMRRY
                     EGDLDGTWHDGLLMDLLADDLDDRPTPTDASDDDLIGSVTLAADEDEAYLGTGAAWRV
                     "
     gene            complement(648..1712)
                     /locus_tag="VHM48_11105"
     CDS             complement(648..1712)
                     /locus_tag="VHM48_11105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX2756005.1"
                     /translation="MSVPRPVVVRQPELVDPPADHAPDHEHAHAHAHAHEALAPHGGR
                     YDSILDAIGHTPLVAIPRMSPNPAVRIYAKLEMVNPTGSVKDRVAKYLVDDLETRGLL
                     HADSIILEPTSGNTGIALAMIGRRKGYRVALVMPDNVTNERRLMAALYGAEVIDSPGH
                     LGSNGAIALAKHLVSKDPRFVMPYQYGNDANPRAHEETTGPEILADCPEIDVFVAGLG
                     TSGTLMGVGRFLRREKPGVRIVAAEPLPGDNVQGLRSLEEGFVPEILDPAMLDAKYLV
                     SNLDAIAALRDLVEREGIFGGPSCGAVLVAAAREARTMTSGTIVALLPDGGWKYLSAG
                     TFSRDFADMADDLEGGVSWW"
     gene            complement(1716..1988)
                     /locus_tag="VHM48_11110"
     CDS             complement(1716..1988)
                     /locus_tag="VHM48_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2756006.1"
                     /translation="MSVVKIPTVLRPQVGGNKEVELAGATVGEVVDALTTAYPSLKSQ
                     LLTVDGELNRFVNVYVNGQDVRYLDGLATVVAERDEVRLLPAMAGG"
     gene            complement(1985..3187)
                     /gene="moeB"
                     /locus_tag="VHM48_11115"
     CDS             complement(1985..3187)
                     /gene="moeB"
                     /locus_tag="VHM48_11115"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX2756007.1"
                     /translation="MPRTFADLLSEARAQIREVTPQQVDALPPGDAVLVDVREESEWE
                     QGYLPGAIHISKGYVEQAIEAEVPDRDRQVILYCAGGIRSLFAAQALERLGYTNVASM
                     SGGFQQWKSAGFRWETPVILSPEQKQRYSRHLLIPEVGAAGQARLLGSKALFIGAGGL
                     GSPAALYLAAAGVGTIGIVDFDVVDLSNLQRQILHTNDRVGERKVESAKKTINALNPD
                     VTVIPHEEMLVAGNVDRIIAGYDVIVDGTDTFETRYLLNDAAVAKNIPVIHASVFRFE
                     GQLTTFIPYEGPCYRCLYPTPPPPELAPGCSVAGVLGVVPGIMGMLQANEALKVLLGI
                     GDTLSGRLLLFDALDATFTELKLRRDPNCPVCSTEAVAARAEGRPLAIPSFAAPAADA
                     PFALGGPA"
     gene            complement(3385..3783)
                     /locus_tag="VHM48_11120"
     CDS             complement(3385..3783)
                     /locus_tag="VHM48_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2756008.1"
                     /translation="MDDLYRDYILEHYRRPHNFGVIEEPTASFEGSNPLCGDRITMQL
                     GIENGVVERVGFTGRGCAISQASASLLTDEIKGKPVADVVGFRADDLLDLLGIDISPA
                     RLKCAMLSHDTLQKALAETETAPASEGATA"
     gene            complement(3866..4039)
                     /locus_tag="VHM48_11125"
     CDS             complement(3866..4039)
                     /locus_tag="VHM48_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756009.1"
                     /translation="MAIQHEVLATPGEAPDVVALTLIRAALRRTRLAASRIAPSKSPQ
                     WMPRTDTRAQDLD"
     gene            complement(4075..>5015)
                     /locus_tag="VHM48_11130"
     CDS             complement(4075..>5015)
                     /locus_tag="VHM48_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756010.1"
                     /translation="DAVSAFTALGLAGTPAGSAWWLDVETSNSWRSDVTLNVAALSGA
                     VYYLGSVAQVGSLGFYSTSYQWGVITGGTSAFSAYPSWVAGAADPAGAAANCIGSGFT
                     GGGVALAQYPSGGFDANLRCSTATPVLTTITVAPANVLVQAGTTQQFTATGVDQFGQP
                     LSPQPPFTWSVSGGGTISNGGLFTAGSTGGGPFMMTATSGSMSGTASVTVTSLVADFS
                     LSVSPPSVSVRRGRTATYTVTVKPSNGFSGSVTLGLSGQPSGSTVTFSPNSTTGTSTL
                     SVRTPSSGPRGTFTLTITGKSSTLTRTATARLSVTK"
BASE COUNT          710 a         1660 c         1863 g          782 t
ORIGIN      
        1 gacgcgccag gcggctcctg tgccgaggta ggcctcgtcc tcgtcggccg cgagggtgac
       61 gctaccgatc aggtcgtcat ctgatgcgtc cgtcggcgtt ggccgatcgt ccagatcgtc
      121 ggcgagtagg tccatcagga gcccatcgtg ccacgtcccg tcgaggtcgc cctcgtagcg
      181 gcgcatgatc ccgaccggcc ggaagccgac ccggcggtag gcccggatcg cccgctcatt
      241 ggcggccgcg ggatcgatcg tcagacggtg gtggcggcgc acgtcgatga gccagcgggc
      301 gacggtccgg accgcgtcgg agccgaggcc gcgatcctgg gcggagccgg ccaggacgat
      361 gtcgatcccg gcatggcggt agcgcgggtc cgtctcctcg tgggcctgga tgacgccgac
      421 gatcacgccg gcttcctcga tggcccatac cgcgacgtgg tcggcccggt cggccagctg
      481 gcgcgcgtcg gcggcagggt cggctgcctg ccaccacgga tggaccgtcg gatccgacag
      541 gatcgtcgtg aacgcgggtg cgtcggccgg ctcagccggc cggaggtgga cccgccggcc
      601 gtcgagcggc acggccgagc ggcgcagcgg cagacgggct gccgggccta ccaccagctg
      661 acgccgccct cgaggtcgtc ggccatgtcg gcgaagtcgc ggctgaacgt gcccgcgctc
      721 aggtacttcc agccaccgtc gggcaggagc gcgacgatcg tgccggacgt catcgtccgg
      781 gcctcgcgcg ccgccgcgac gagcaccgct ccgcatgacg gaccgccgaa gatcccctcg
      841 cgttcgacga ggtcgcgcaa tgcggcgatc gcgtcgaggt tgctgacgag gtacttcgcg
      901 tcgagcatgg ccgggtcgag gatctcgggc acgaagccct cctcgagcga tcgcaggccc
      961 tggacgttgt cacccgggag cggctcggcg gcgacgatcc ggaccccggg cttttcccgt
     1021 cgcaggaacc gcccgacgcc catcagggtc ccgctcgttc cgagcccggc gacgaacacg
     1081 tcgatctccg ggcagtcggc caggatctcc gggccggtcg tctcctcgtg ggctcgcggg
     1141 ttcgcgtcgt tgccgtactg gtacggcatc acgaaccgcg ggtccttcga cacgaggtgc
     1201 ttcgccaggg cgatcgcgcc gttgctcccg agatgccccg gcgagtcgat gacctcggcc
     1261 ccgtacaggg cggccatgag gcgtcgctcg ttggtgacgt tgtcgggcat gacgagcgcg
     1321 acccggtagc ccttccgccg gccgatcatc gcgagcgcga tcccggtgtt gcccgaggtc
     1381 ggctcgagga tgatcgagtc agcgtggagc aggccgcgcg tctcgaggtc gtcgacgagg
     1441 tatttcgcga cgcggtcctt caccgacccg gtggggttca ccatctcgag cttggcgtag
     1501 atccggacgg ccgggttcgg cgacatccgc gggatcgcga cgagcggcgt gtggccgatc
     1561 gcgtcgagga tgctgtcgta gcgcccgccg tgcggagcga gcgcctcgtg ggcatgggca
     1621 tgggcgtggg cgtgttcgtg gtcaggagcg tgatcggcgg gcgggtccac gagctcgggc
     1681 tggcggacga cgaccggcct cggcacggac atcgttcagc cgccggccat cgccggcagg
     1741 aggcggacct cgtcccgctc ggcgacgacg gtcgccaggc cgtcgagata gcggacgtcc
     1801 tggccgttga cgtagacgtt gacgaagcgg ttgagctcgc cgtcgacggt caggagctgg
     1861 ctcttcaggc tcggatacgc ggtcgtgagc gcgtcgacca cctcgccgac cgtcgccccg
     1921 gccagctcga cctccttgtt gcccccgacc tggggacgga ggacggtcgg gatcttgacg
     1981 acgctcacgc gggacctccg agggcgaacg gcgcatccgc ggccggcgcc gcgaacgacg
     2041 ggatggcgag gggccggccc tcggcccggg cggcgaccgc ctccgtcgag cagaccgggc
     2101 agttcggatc gcgccggagc ttgagctcgg tgaaggtcgc gtcgagggca tcgaagagga
     2161 gcagccggcc ggacagggtg tcaccgatcc cgagcaggac cttgagcgcc tcgttggcct
     2221 ggagcatgcc catgatcccg ggcacgacgc cgaggacgcc ggccaccgag cagccgggcg
     2281 cgagctcggg cggtggcggg gtcgggtaga ggcagcggta gcacggcccc tcatacggga
     2341 tgaaggtcgt gagctggccc tcgaaccgga agacgctggc gtggatcacc gggatgttct
     2401 tcgcgaccgc cgcgtcgttg aggaggtagc gcgtctcgaa cgtgtcggtc ccgtcgacga
     2461 tcacgtcgta gccggcgatg atccggtcga cgttgcccgc cacgagcatc tcctcgtggg
     2521 ggatgaccgt cacgtcgggg ttgagcgcgt tgatcgtctt cttggccgac tcgaccttgc
     2581 gctcgcccac ccggtcgttc gtgtggagga tctgccgctg gaggttggac aggtcgacga
     2641 cgtcgaagtc gacgatgccg atcgtaccga cgccggcggc cgcgaggtag agcgccgccg
     2701 gggagccgag accgccggcg ccgatgaaca gggccttcga tccgagcagg cgggcctggc
     2761 cggccgcgcc gacctcgggg atcagcaggt gacggctgta gcgctgcttc tgctccggcg
     2821 agaggatgac cggcgtctcc cagcggaagc ccgcggactt ccactgctgg aacccgcccg
     2881 acatcgaggc gacattcgtg tagccgagcc gctcgagcgc ctgggccgcg aacaggctcc
     2941 ggatcccgcc ggcgcagtag aggatgacct ggcgatcgcg gtcggggacc tcggcctcga
     3001 tcgcctgttc gacgtagccc ttgctgatgt ggatcgcgcc gggcagatag ccctgctccc
     3061 actccgactc ttcgcggacg tcgacgagga cggcgtcgcc cggcgggagg gcgtcgacct
     3121 gttggggtgt gacctcgcgg atctgggccc gcgcctcact gaggaggtcg gcgaacgtgc
     3181 gtggcatggc tgatggggtg ctccgttgac gacaggctgc cggctggtcg tgcggcaggg
     3241 cgaggagaag aacgacgagg atggctgccg gctatgccgg ccggcgacac tcgcagcaat
     3301 cctcgggccg ctggagtcgc ggagctcctc cgcggacctc ggagggcgaa tgccgaccca
     3361 tcggggaggt tgccagtgcg ccgatcatgc tgttgctcct tccgatgccg gtgccgtttc
     3421 ggtctcggcg agcgccttct ggagggtgtc gtggctgagc atcgcgcact tcaggcgggc
     3481 cgggctgatg tcgatcccga gcaggtcgag gaggtcgtcg gcccggaagc cgaccacgtc
     3541 cgcaacgggc ttgcccttga tctcgtcggt caggaggctg gcgctggcct ggctgatcgc
     3601 gcagccgcgg cccgtgaacc cgacccgctc gacgacgccg ttctcgatcc cgagctgcat
     3661 cgtgatccgg tcgccgcaca gcgggttgct gccctcgaag gacgcggtcg gctcctcgat
     3721 gaccccgaag ttgtggggcc gtcggtagtg ctcgaggatg tagtcgcggt agaggtcgtc
     3781 catcggtttc cggttcagct gtcgtcccgg gtcaatcccg aggaatttcg tcgggattga
     3841 tgctatggcc gcgcgccggt cgtcgtcaat cgagatcctg ggcgcgggtg tcagttcgcg
     3901 gcatccactg aggcgatttg ctcggggcga tccgcgatgc agctaatcgg gttcgtcgaa
     3961 gagccgcccg gatgagcgtc agcgcgacca cgtccggcgc ctcgccgggc gtcgcgagga
     4021 cctcgtgctg gatcgccatc gcggacgagc cgccagaccg ggcgacgagc gcttctactt
     4081 cgtgacgctg agcctggcgg tggctgtcct cgtgagggtg ctgctcttcc ccgtgatggt
     4141 cagcgtgaag gtacccctcg gcccggacga cggcgtcctc acgcttagcg tcgatgtgcc
     4201 ggtggtgctg ttcggcgaga aggtgacggt agagcccgac ggctggccgc tgaggccaag
     4261 ggtgactgac ccggagaagc cgttggacgg cttgacggtc acggtgtagg tagcggtacg
     4321 accgcgccgg acggataccg aaggtgggct cacggagagc gagaagtccg ccaccaggct
     4381 cgtgaccgtc acgctcgccg taccgctcat gctgccgctc gtcgccgtca tcatgaacgg
     4441 tccgccgccg gtcgagccgg cggtgaacag gccaccgttg ctgatcgtgc cgccgccgct
     4501 caccgaccag gtgaacggcg gctgcgggct caacggctgg ccgaactgat cgacgccggt
     4561 ggccgtgaac tgctgggtcg tgcccgcctg caccaagacg ttggcaggcg cgaccgtgat
     4621 ggtggtgagg acgggggtgg ccgtcgagca gcggaggttc gcgtcgaacc cacccgaggg
     4681 gtactgggcg agtgcgacgc cgccccccgt gaacccagag ccgatgcagt tcgcggccgc
     4741 gcccgccggg tccgctgctc cggcgaccca gctcgggtac gcgctgaagg cgctcgtccc
     4801 gccggtgatc acgccccact ggtagctcgt cgagtagaag ccgagcgacc cgacctgggc
     4861 gacgctgccg aggtagtaca cagcaccgga gagcgcggcg acattgaggg tgacgtcgga
     4921 ccgccagctg ttcgacgtct cgacgtcgag ccaccacgcg ctgcccgccg gagtacccgc
     4981 gaggccgagc gcggtgaacg cagagaccgc gtcgg
//
LOCUS       DATAIW010000370         6873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_189304, whole genome shotgun sequence.
ACCESSION   DATAIW010000370 DATAIW010000000
VERSION     DATAIW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189304"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="eno"
                     /locus_tag="VHM48_11135"
     CDS             complement(<1..791)
                     /gene="eno"
                     /locus_tag="VHM48_11135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2756011.1"
                     /translation="MNTVIELVDAREILDSRGNPTIEVDVILGDGSVGRAAVPSGAST
                     GAHEAVELRDGDKARFGGKGVLTAVRNVTERIAPELYGMDAADQAGVDDLLRELDGTP
                     NKATLGANAILGVSLACAHAAAAAHDEPLYRYLGGVGARILPVPMFNILNGGKHAQDS
                     TDFQEFMVMPVGSETFSEALRAGAEIFAALRGILHDEGHTTGQGDEGGFAPSLPSNAA
                     AVEVILRAIEKAGYRPGEDVAIALDPATSSILVEGTGIDDVTGQYR"
     gene            complement(788..2032)
                     /locus_tag="VHM48_11140"
     CDS             complement(788..2032)
                     /locus_tag="VHM48_11140"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2756012.1"
                     /translation="MEKLTIRDFDAAGKRVFVRVDFNVPLADGKVTDDSRIRAALPTI
                     TTLLNAGARVILASHLGRPDGKVQDGLRLRPVGERLSQLLRRNVPVTGDALGAGTEDA
                     VRRMRNGEAILLENLRFHAAEEKNDPAFAKALASYAEIYVNDAFGTAHRAHASTVGIA
                     SFLPAYAGLLMEREIAALSSLLEHPRRPFAAILGGAKVSDKIGVIDHLLTKIDLLILG
                     GGMANTFLLAQGKSVGKSLAEHDRVDDARRILAAAEKAGVKVLLPVDVVIAKEVTRGT
                     EYKTVGTEKFPASWHIVDLGEESRTAIEVALADARTVLWNGPVGVFEIPSFAHGTRAI
                     ARFLAGRAEHGVTVVVGGGDSVAAIEHEGLTAKMTHISTGGGASLEFLEGRELPGIAV
                     LQDRPADEPAAKAKKAKAGAKA"
     gene            complement(2178..3200)
                     /gene="gap"
                     /locus_tag="VHM48_11145"
     CDS             complement(2178..3200)
                     /gene="gap"
                     /locus_tag="VHM48_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2756013.1"
                     /translation="MTVRVGINGFGRIGRQSLKALIERTPDVEVVAVNDLVETSLNAL
                     LFKHDSTYGAFPGTVDHTDDALIIDGREIKVLKEKDPAALPWGDLGVDIVLESTGIFT
                     TAEKAGAHIAAGAKKVIISAPAKGEDITIVLGVNESKYDPASHHIISNASCTTNCLAP
                     AAKVVHDLLGIQRGLMNTIHSYTNDQRILDVAHKDPRRARAAGLNIIPTTTGAAKALA
                     LVIPDLKGKFDGFSLRVPTPTVSVVDFTADVARPTSVEELNAAFRTAAEGPLKGILGV
                     SDDPLVSTDFRGDARSSIIDAASTMVLDGTMVKVIAWYDNEWGYSCRVADLIGFVAAR
                     LPVAAA"
     gene            complement(3547..3687)
                     /locus_tag="VHM48_11150"
     CDS             complement(3547..3687)
                     /locus_tag="VHM48_11150"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX2756014.1"
                     /translation="MAEAYCVKDKMKVEVVNPEKITMKNGKPALQGTCPKCGGKVFKI
                     GG"
     gene            complement(3749..4609)
                     /locus_tag="VHM48_11155"
     CDS             complement(3749..4609)
                     /locus_tag="VHM48_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX2756015.1"
                     /translation="MRDEVLPNADPRVALPIGERQYHIGLGPGDLAEYILLPGDPDRT
                     SRIAARLENVEFERRHREFASVTGTYRGTRVSVVSTGIGTDNVEITVAEILAITERPT
                     FVRVGSCGSLQPEISLGDLIVSTGAVRLESTTSFFVHDGFPAVADYEAVLALIEAAEQ
                     LGHRTHVGLTATAPGFFGAQGRPIPQLPIRYPDLAEEMARQRVMNFEMEASALLVLAT
                     LARCRAGVVCAVYAQRTTGAFIEGEAKDAAEAACVETGLEGLLILAEMDRQKRAAGTD
                     RWRPSLWNRT"
     gene            complement(4755..6761)
                     /locus_tag="VHM48_11160"
     CDS             complement(4755..6761)
                     /locus_tag="VHM48_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX2756016.1"
                     /translation="MVDSRVTVGGVVTAGAGRLGTPALIAIQDSTAGIVVRLADTTPR
                     PSVGTWIELTGTLADPYGQLELRAIAGVRNVGPAALPAPAAVDGATLVEGVEGRLVSV
                     EGVAQGRPVKSTSGDLTFVVTTAHGPVRIAADASAGLSTSSVATGDRLRLTGVAGQRA
                     SHKDALDGYRIWIRGTSDIVRLGSPSPSTTPSPSPTSTAGHSTTSDTIRTIAAAILAG
                     SGTLTIEGSVTTSASLLDATNRRLIVQDRTAAIEVLLPAGEGAPPIGTRIRVNGEVGR
                     AYGAPRIRAATIRRLGTAVVSPLELRVAPGAAHEWRLVRVRGDVVEIHRSGDRWTAEL
                     LVGGTRVPIVGLAGAAIPSAALGAGRTATVVGIVRRPYPSASDRRFAIMPRSSRDLTV
                     GAAADDGSAAAGSAGPGSSGAGATGAGSTGVTGPDAGSGSAAGGPRDIDLAALAAHVG
                     ETVRVGGLVQSVDGDGFRLDDGTAIASVRLRAAAVDIAGSVLVGDALSATGRVELAPS
                     GGAAIVAVEDPAGIALVGDLGAVDPTDSTGAGTDEALTGPSAASDGPVAEPASRITAG
                     LADPAIPEVGALGIVLVALASLTLTMLRRRRSRRRMAARIADRLAAIGGAPGLVRSIA
                     AAALAGGATRPAAIALGGPSVARNGPEPGGDTGRSNGHSEAEGR"
BASE COUNT          942 a         2367 c         2501 g         1063 t
ORIGIN      
        1 cggtactggc cggtgacgtc gtcgatcccg gtgccctcga cgaggatcga gctggtcgcc
       61 gggtcgagcg cgatcgcgac gtcctcgccg ggccggtagc cagccttctc gatcgcccgg
      121 aggatgacct cgaccgcggc cgcgttcgac ggcagcgacg gggcgaaccc gccctcgtcg
      181 ccctggcccg tcgtgtgacc ctcgtcgtgg aggatcccgc gcagcgcggc gaagatctcg
      241 gccccggccc gcagcgcctc ggagaacgtc tccgacccga cgggcatgac catgaactcc
      301 tggaagtcgg tcgagtcctg ggcgtgcttg ccaccgttga ggatgttgaa catcgggacc
      361 ggcaggatcc gcgcgccgac accgccgagg tagcggtaga gcggctcatc gtgggccgcc
      421 gccgcggcgt gggcacaggc gagcgacacg ccaaggatcg cattcgcccc cagggtcgcc
      481 ttgttcggcg tgccatcgag ctcgcggagg aggtcgtcga caccggcctg gtcggcggcg
      541 tccatcccgt acagctccgg cgcgatccgc tcggtcacgt tgcggaccgc cgtcaggacg
      601 cccttgccgc cgaaccgtgc cttgtccccg tcgcgcagct cgaccgcctc atgggcgccc
      661 gtcgacgcgc ccgatggaac ggccgcccga ccgaccgatc cgtcgccgag gatcacgtcg
      721 acctcgatgg tcggatttcc gcgcgagtcg aggatctcgc gtgcgtcgac gagctcgatg
      781 accgtgttca cgccttcgct cctgccttgg ccttcttcgc tttcgcggcc ggctcgtcgg
      841 ccggtcgatc ctggagaacg gcgatcccgg gcagctcgcg gccctcgagg aactcgagcg
      901 aggcgccgcc gccggtgctg atgtgggtca tcttcgcggt caggccttcg tgctcgatcg
      961 cggcgacgct gtcaccgcca ccgacgacga ccgtcacgcc atgctcggcc ctgccggcga
     1021 ggaaccgggc gatggcccgc gtcccgtggg cgaaggacgg gatctcgaag accccgaccg
     1081 gcccgttcca gagcaccgtt cttgcgtcgg cgagggcgac ctcgatcgcg gtgcggctct
     1141 cctcgccgag gtcgacgatg tgccacgagg ccgggaactt ctcggtcccg acggtcttgt
     1201 actcggtccc gcgggtgacc tccttggcga tcacgacgtc gacgggcagg agcaccttga
     1261 ccccggcctt ctcggccgcg gcgaggattc ggcgagcatc gtcgacgcga tcgtgctcgg
     1321 ccaggctctt gccgacggac ttgccctggg cgagcaggaa cgtgttggcc atcccgccgc
     1381 cgaggatcag gaggtcgatc ttcgtgagga ggtggtcgat caccccgatc ttgtcgctga
     1441 ccttggcccc gccgagaatc gcggcgaagg gtcgcctcgg gtgctcgagg aggctgctca
     1501 aggcggcgat ctcacgctcc atcaggagac cggcgtaggc cggcaggaac gaggcgatgc
     1561 cgacagtcga ggcatgggcg cggtgggcgg tcccgaatgc gtcgttgacg tagatctcgg
     1621 cgtacgaggc gagcgccttc gcgaacgccg ggtcgttctt ctcctcggcc gcgtggaagc
     1681 ggaggttctc gaggagaatc gcctccccgt tccgcatccg cctgacggcg tcctccgtcc
     1741 cggccccgag cgcgtcgccg gtcacgggca cgttccgccg gaggagctgg ctgaggcgct
     1801 cgccgacggg ccgcaggcgc aggccgtcct ggaccttgcc gtccggacgc cccagatggc
     1861 tcgcgagaat gacccgcgcg ccggcgttga ggagggtcgt gatcgtcggt agcgcggccc
     1921 ggatccgcga atcgtcggtg accttgccgt ccgcgagcgg gacgttgaaa tcgacgcgca
     1981 cgaagacgcg cttgcccgcg gcgtcaaagt cgcggatcgt cagcttttcc atcttgtcct
     2041 gcgctggagc ggcggatcgg cggacccgcg gcggggatgg cgtggatggc tcaggccgtg
     2101 cggccgtcgt ccgtcgtccg tcggcccggg tgaaggtgcc cgggccggcg ctggccgacg
     2161 cgacggacga gccggtctca ggctgcggcg acgggcagcc gggcagcgac gaagccgatc
     2221 aggtcggcga cgcggcagct gtagccccac tcgttgtcgt accaggcgat gaccttgacc
     2281 atggtgccgt cgaggaccat ggtcgaggcg gcgtcgatga tcgaggagcg ggcgtcgccg
     2341 cggaagtcgg tcgagacgag cggatcgtcg ctgaccccga ggatgccctt gagcgggcct
     2401 tcggcggcag tccggaacgc ggcattgagc tcctcgaccg aggtcgggcg ggcgacgtcg
     2461 gcggtgaagt cgacgacgct gacggtcggg gtcgggacgc gcaggctgaa gccgtcgaac
     2521 ttgcccttga ggtccgggat gaccagggcg agcgccttcg ccgcgccggt cgtcgtcggg
     2581 atgatgttca ggcccgcggc gcgggcccga cgcggatcct tgtgggcgac gtcgaggatg
     2641 cgctggtcgt tcgtgtagct atggatcgtg ttcatcaggc cgcgctggat gccgagcagg
     2701 tcgtggacga ccttggctgc gggagccagg cagttcgtcg tgcagctggc gttgctgatg
     2761 atgtggtggc tggccgggtc gtacttgctc tcgttgacgc cgaggacgat cgtgatgtcc
     2821 tcgcccttgg ccggggcgct gatgatcacc ttcttggcgc cggccgcgat gtgggcgccg
     2881 gctttctcgg cggtggtgaa gatgccggtc gactcgagga cgatgtcgac gccgaggtcg
     2941 ccccacggga gcgcggccgg atccttctcc ttgaggacct tgatctcgcg cccgtcgatg
     3001 atcaatgcgt cgtcggtgtg gtcgaccgtg cccgggaagg cgccgtacgt cgagtcgtgc
     3061 ttgaagagca gggcgttgag cgatgtctcg acgagatcgt tgacggccac gacttcgacg
     3121 tccggggtcc gctcgatcag ggccttgagc gactgacggc cgatccgtcc gaagccgttg
     3181 atgccgacgc gcacagtcat gattcctgtc ctccgcggtc acgagcgccg gctggcgccc
     3241 ggtgttgtcc gtcatccggg tcagcgagag gtcgggcgcc atgtcgggcg cacgcgaggc
     3301 ctcccgtcgt acggtgccat cgtacgacgt cgcggagtca gagccgtccg gcccggaggt
     3361 tccgggccgg ttgtcgatag gttgcgcctc ggcgtcgtgc cgcatcgagg gtcgcgaccg
     3421 ggagcgagtc gagaccgggt cggggccgat ttcgggaccg cgacggcggc gcagccgccc
     3481 cgggaatcga gcgacccccg ccgttgccga cgggggtcgc gttcgacgtt cgaactcggg
     3541 actgcgtcag ccgccgatct tgaagacctt gccaccgcat ttcgggcagg tgccctggag
     3601 cgccggcttg ccgttcttca tcgtgatctt ctcgggattc acgacttcga ctttcatctt
     3661 gtccttgacg caataggctt cagccatgga gcgggctcct cctcgggtca gcgtggcgtc
     3721 acgggaaccc tcgtcccgtg tccgcttctc acgttcggtt ccagagcgac ggtcgccagc
     3781 gatccgtccc tgctgctcgc ttctgtcgat ccatctcggc caggatcagg agcccttcga
     3841 gccccgtctc cacgcacgcc gcctctgccg cgtccttggc ttctccctcg atgaacgctc
     3901 cggtggtgcg ctgggcgtag accgcacaga ccacaccggc ccgacatcgg gcgagagttg
     3961 ccaggaccag aagcgccgac gcctccatct cgaagttcat gacgcgctgg cgcgccatct
     4021 cctccgccag gtcagggtaa cggatgggca gttgcgggat ggggcgccct tgcgccccga
     4081 agaaccccgg tgccgtggcc gtcaacccga cgtgggtccg atgaccgagc tgctccgctg
     4141 cctcgatgag cgccaggacc gcctcgtagt cggcgaccgc tggaaatcca tcgtggacga
     4201 agaagctcgt cgtcgactcg agccggactg ctccggtcga gacgatcagg tccccgaggg
     4261 agatctccgg ctggaggctc ccgcaggatc cgacccggac gaaggtcggc cgttccgtga
     4321 tcgccaggat ctccgccacc gtgatctcga cattgtccgt accgatcccg gtcgagacga
     4381 ccgagacccg ggtgccgcga tacgttccgg tgactgacgc gaactcccgg tggcgccgct
     4441 cgaactcgac gttttcgaga cgcgcggcga tccgtgaggt ccggtcgggg tcaccgggga
     4501 ggaggatgta ctcggccagg tcacccggtc cgagcccgat gtggtattgc cgttctccga
     4561 tggggagggc tacgcgggga tccgcgttcg gcaagacctc gtccctcact ctggcgtgct
     4621 tcgcaggcgc gaaactcggg cgaggataga cccgcattct cgcgagcgtc aagcacggcc
     4681 acgcacgaac cacggacgaa ccacgcactg gccacgctcc gcggggccgg cgcgcaccga
     4741 tcggcgaccg ccgctcaccg gccctccgct tcgctgtggc cgttgcttcg accggtatca
     4801 cctcccggtt ccggtccgtt gcgcgcgacc gacgggcccc cgagggcgat cgccgcgggc
     4861 cgtgtcgctc ctccagccag ggccgccgcc gcgatgctcc gcacgagtcc tggagcacca
     4921 ccgatggcgg cgaggcgatc ggcgatccgg gcagccatgc gtcggcgcga acgccggcgg
     4981 cggagcatcg tcagcgtcag cgaggccagc gcgacgagga cgatcccgag ggcaccgacc
     5041 tcggggatgg cgggatcggc aagtccggcc gtgatcctgg aggcgggttc ggcaacgggg
     5101 ccgtctgacg ccgcggacgg gcctgtcaag gcctcgtcgg tgcccgcacc ggtcgagtcg
     5161 gttggatcga ccgctccgag gtcgccgacg agggcgatcc ccgccgggtc ttcgaccgcg
     5221 acgatcgccg cgccaccgga cggagcgagc tcgacgcgcc cggtcgcgct caacgcgtcg
     5281 cccacgagga ccgaacccgc gatgtccacc gcggccgcgc ggagtcgcac cgaagcgatc
     5341 gcggtcccgt cgtcgaggcg aaatccgtcg ccgtcgaccg actggacgag gcccccgacc
     5401 cggaccgttt cgccgacatg cgccgcgagt gccgccaggt cgatgtcacg cggaccgccg
     5461 gcggccgagc cggatccggc atcggggccg gtcacacccg tcgagccggc gccggtcgcg
     5521 ccggcccccg acgagcccgg tccagccgac ccggcggcgg ccgagccgtc gtcggctgca
     5581 gcgcccaccg tcaggtcgcg ggatgaccgc ggcatgatcg cgaatcgccg atccgacgcc
     5641 gacgggtacg gtcggcggac gatcccgacg actgtcgctg ttcgaccggc cccgagcgcc
     5701 gccgacggga tggcggcgcc ggcgagaccg acgatcggga cgcgcgtgcc gccgacgagc
     5761 aactcggccg tccagcggtc gcccgagcgg tggatctcga cgacgtcgcc ccggacccgg
     5821 acgagccgcc actcgtgcgc cgcaccgggc gcgacgcgga gttcgagcgg cgagacgacg
     5881 gccgtgccga gacgtcggat ggtcgcggcg cggatcctcg gggcgccgta cgcccggccg
     5941 acctcgccgt tgacacggat ccgggtgccg atcggtgggg cgccctcgcc cgccgggagc
     6001 agaacctcga tggccgcagt ccggtcctgg acgatcagcc ggcggttcgt ggcatcgagc
     6061 agggacgcgg aggtcgtgac cgagccctcg atcgtgagcg tcccgctgcc cgcaaggatc
     6121 gcggcggcga tcgtgcggat ggtgtcgctc gtcgtcgagt gcccggcggt cgatgtcggc
     6181 gacggcgacg gcgtggtgga cggcgagggc gaaccgagtc ggacgatgtc gctcgtgccg
     6241 cggatccaga tccggtagcc gtcgagtgcg tccttgtggc tggcccgctg gccggcaacg
     6301 ccggtcaggc ggaggcggtc tccggtcgcg accgagctcg tcgagagtcc ggcggacgcg
     6361 tcggccgcga tccggaccgg gccgtgggcc gtcgtcacga cgaaggtgag gtcgccgctc
     6421 gtcgacttga ccggccgtcc ctgggcgaca ccctcgaccg agacgagccg accttcgacg
     6481 ccctcgacga gcgtcgcgcc gtcgactgcg gcgggcgccg gcaacgctgc cggtccgaca
     6541 ttccgaacgc cggcgatcgc ccgcagctcg agctggccgt aggggtccgc gagcgtcccg
     6601 gtcagctcga tccaggtgcc gaccgaggga cgcggcgtgg tgtccgcgag acgcacgacg
     6661 atccccgctg tcgagtcctg gatcgcgatg agggccggcg tcccgagccg accggccccg
     6721 gccgtcacga caccgccaac ggtgactcgg ctgtcgacca cggacgatcg tgctgccgcg
     6781 atcggactgg ccggtgcggc ggacggcgtg gacgtcggag tcggcgcggg tgtcggggtc
     6841 ggaacgggtg tcggggtcgg aacgggtgtc ggg
//
LOCUS       DATAIW010000371         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_23874, whole genome shotgun sequence.
ACCESSION   DATAIW010000371 DATAIW010000000
VERSION     DATAIW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23874"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VHM48_11165"
     CDS             complement(<1..360)
                     /locus_tag="VHM48_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756017.1"
                     /translation="MTLDRDAAATEALLTDLYLDALLAGVVLDAAAAGTPHDAVGRLD
                     PAARRAAERLRHDLVRVHPSFRFEERLATRLAEAAVALRVPVAAGAEGRIVAFRPGRP
                     IDLDPDLLPTDASPDPSD"
     gene            complement(357..1025)
                     /locus_tag="VHM48_11170"
     CDS             complement(357..1025)
                     /locus_tag="VHM48_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756018.1"
                     /translation="MNVSGRVTAVSAVRPARIAHLDRDRPLVEAAQRDLLKFDALYRR
                     YLAQVYSYAFYELGDHHEAEDATERTFLAALANLGRFEERARPADGEGASTFRVWLFQ
                     IARNAVANQRRGRRRHPQAPLEAAALVADPLDIEGAVTIRDEAAAAWRAVGRLPSDRR
                     RALVLRFVEEMSTAEIAGILGRSEGAVRVLIHRALRTVARDLGGRAASGPVDASASPD
                     AERP"
     gene            complement(1054..1932)
                     /locus_tag="VHM48_11175"
     CDS             complement(1054..1932)
                     /locus_tag="VHM48_11175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2756019.1"
                     /translation="MTRVAAIPALRRHERFASVGSTSDVVRAWLAAGEPEICLALADE
                     QTAGRGRNGRTWTAPPGAGLLVSLGFRPSWLAADRAWRLAATVSLAMADAAEEVAGLP
                     DRSVRLKWPNDLVIATDSAGRALGPLGLDDAGTGGRSAATVRKLGGVLGETEGLGTDD
                     PRVIVGIGLNADWPAAKFPAELTGTMTSLRSASGERPIDLALLLDAFTSRLETRVEAL
                     FGGRFDVADWVERQITTGRRIDLVRPDETVETVLARGVDGVGGGLRIAAPTDADPDTE
                     RTVVVGEVRHVRLARV"
     gene            complement(1929..3236)
                     /locus_tag="VHM48_11180"
     CDS             complement(1929..3236)
                     /locus_tag="VHM48_11180"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2756020.1"
                     /translation="MPAAPLVVQKFGGSSLADADRIRRVARRIARERAAGSDLVVIVS
                     AMGDTTDELLGLAAAITDTPDERELDMLLATGEHQSATLVSMALHALGVAAISLSGPQ
                     AGITTDGHYGRARIASIEPARVRTELERGKVVIVAGFQGRAAGEDVAAADITTLGRGG
                     SDTTAVALAARLGADRCQIFTDVRGIYTADPRLVPDARQLAIIGYEEMLELAHQGAQV
                     MQTRAVELGWVNDVVIEVLSSFEDSPGTLIKEDPLVEQRNKVRGLAHDRNVAKVTIVA
                     VPDRPGIARSLFDPLAEAGVNVDMIVQNVGHGGETDLSFTVPQAELGKTKKALESVVR
                     DLGAREMTTDASVAKVSIVGAGLHNAPGYAARMFGTLADAGVNIEMISTSEVRITCMI
                     AEEELETALRALHDAFELARPESAAVESATASQNTAGGSSASR"
     gene            complement(3416..>4055)
                     /locus_tag="VHM48_11185"
     CDS             complement(3416..>4055)
                     /locus_tag="VHM48_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756021.1"
                     /translation="GELLRIAWADLVAVAEAVGARLDLDIRWQGERIDRLLDERHSAT
                     ADVLVGLLRRYGWMAEVEVSFAIYAERGSIDVVGRHPLTRMIATFEVKASIGDANQTI
                     IGVDRKARLAPEIARERGWVCRGVARFLVIADSTTSRSRVTRHAEIFGTAFPLRGRDC
                     VAWLRSPTTPPPSGLFFVNVPNDHTTGATRQRVRRSAVHRAAPSGTARSGEP"
BASE COUNT          563 a         1431 c         1487 g          574 t
ORIGIN      
        1 gtcgctcggg tccgggctcg cgtcggtcgg gagcaggtcg ggatcgagat cgatcggcct
       61 gcctggtcgg aaggcgacga tccgaccttc cgcgccggca gcgacgggca ctcgcagcgc
      121 tacggcggcc tcggcgaggc gcgtcgcgag gcgctcctcg aagcggaacg acggatggac
      181 acggacgagg tcgtggcgga ggcgctcggc cgcgcgacgg gcggccggat cgagccggcc
      241 gaccgcgtcg tgcggcgtgc cggccgccgc tgcatcgagg accacgccgg ccagaagggc
      301 gtcgagatac aggtccgtca gcagggcctc ggtcgcggcc gcatcacgat cgagggtcat
      361 ggccgctcgg cgtcaggcga ggccgaggcg tcaaccggcc ccgacgcggc gcgcccgcca
      421 agatcgcggg cgacggtccg cagggctcga tggatcaaga cccgaaccgc gccctccgac
      481 cggccgagga tcccggcgat ctcggcggtc gacatctcct cgacgaaccg caggacgagc
      541 gctcgccgcc gatcggacgg taggcggccg acggcccgcc atgccgcggc cgcctcgtca
      601 cggatcgtga cggcaccttc gatatcgagc gggtcggcga cgagcgcagc cgcctcgagc
      661 ggggcctggg gatgccgccg ccgcccgcgg cgctggttgg cgaccgcgtt ccgggcgatc
      721 tggaacagcc agacgcggaa cgtcgacgct ccctcgccgt cggccggtcg ggcccgctcc
      781 tcgaaccggc cgaggttcgc gagcgcggcg agaaatgtcc gttcggtggc gtcctcggcc
      841 tcgtgatgat cgccgagctc gtagaaggcg tagctgtaca cctgggccag atacctgcgg
      901 tacagggcgt cgaacttgag gaggtcgcgt tgggccgcct ccaccaacgg ccggtcgcga
      961 tcgagatggg cgatccgggc cgggcgcacg gccgagaccg ctgtcacccg tccggagacg
     1021 ttcatccttc ccaaacccgg tcgagccatc ccgttacacc cgggccagtc gaacatgccg
     1081 gacctcgccg acgacgacgg tccgttcggt gtcgggatcc gcgtccgtcg gagcggcgat
     1141 ccggagcccg ccgccaaccc cgtccacgcc ccgggcaaga accgtctcga cggtctcgtc
     1201 cggccggacc aggtcgatcc ggcgaccggt cgtgatctgg cgctcgaccc agtccgcgac
     1261 gtcgaagcgg ccgccgaaca gggcctcgac ccgggtctcg aggcgggagg tgaatgcgtc
     1321 gagcaggagg gcgaggtcga tgggccgctc gccggacgcc gaccgaagtg aggtcatcgt
     1381 tccggtgagc tccgccggga acttcgccgc gggccagtcg gcgttcaggc cgatccccac
     1441 gatcacgcgc gggtcgtccg ttccgaggcc ctccgtctcg ccgaggaccc cgccgagctt
     1501 gcggacggtg gcagccgagc gtccgcccgt gcccgcgtcg tcgaggccga gcgggcccag
     1561 ggctcggccg gccgaatcgg tcgcgatcac gaggtcattc ggccacttga ggcggacgga
     1621 ccggtccggc aggccggcga cctcctcggc cgcgtcggcc atcgcgagcg acaccgtggc
     1681 ggccaggcgc caggcccggt cggcggcgag ccacgacggc cggaagccga gcgagacgag
     1741 gagtccagcg cccggtggag cggtccacgt ccggccgttc cggccgcgac ccgcggtctg
     1801 ctcgtcggca agggcgaggc agatctcggg ctcaccggcg gccagccatg cccggacgac
     1861 gtcgctggtc gagccgacgc tggcgaaccg ctcgtggcgg cgcagggcgg gtatcgcggc
     1921 gacgcgggtc acctggacgc ggagctcccg ccggcggtgt tctggcttgc cgttgccgat
     1981 tcgacggccg ccgactcggg ccgcgcgagc tcgaaggcgt cgtgcaacgc gcgcagcgcc
     2041 gtctcgagct cttcctcggc gatcatgcac gtgatccgga cctcggatgt cgagatcatc
     2101 tcgatgttca cgccggcgtc ggcgagcgtc ccgaacatcc gcgcggcata gcccggggcg
     2161 ttgtgcagcc cggcgccgac gatgctgacc ttcgcaaccg aggcgtcggt cgtcatctcc
     2221 cgggcgccga gatcgcggac gacgctctcg agcgcctttt tcgtctttcc gagctcggcc
     2281 tggggaaccg tgaacgacag gtctgtttcg ccgccgtggc cgacgttctg gacgatcatg
     2341 tcgacattga ccccggcctc ggccagtggg tcgaacaggc tgcgggcgat ccccggccgg
     2401 tccgggaccg cgacgatcgt gaccttggcg acgttccgat cgtgggcgag gccacggacc
     2461 ttgttccgct gctccacgag cgggtcctcc ttgatcagcg ttccggggga gtcctcgaat
     2521 gagctgagga cctcgatgac cacatcgttg acccagccga gctcgaccgc ccgcgtctgc
     2581 atcacctggg caccctggtg ggcgagctcg agcatctcct cgtagccgat gatcgccagc
     2641 tggcgggcat cggggacgag ccgcgggtcg gccgtgtaga tcccgcggac gtcggtgaag
     2701 atctggcagc ggtcggcgcc gagacgggcg gcgagggcga ccgcggtcgt gtcgctgcca
     2761 cctcgaccga gggtcgtgat atcggcggcc gcgacgtcct cgcctgcggc ccggccctgg
     2821 aagccggcaa cgatgacgac cttgcctcgc tcgagctccg tccggacgcg agccggctcg
     2881 atcgaggcga tccgggcccg gccgtagtga ccgtcggtcg tgatcccggc ctgcggtccg
     2941 ctgaggctga tcgccgcgac gccgagggca tgcagggcca tcgaaacgag cgtcgcgctc
     3001 tggtgctcgc cggtcgcgag gagcatgtcc agctcgcgct cgtcgggcgt gtcggtgatc
     3061 gcggcggcga ggccgaggag ctcgtcggtg gtatcgccca tcgcggacac gatgacgacg
     3121 agatccgacc cggccgctcg ctcgcgggcg atccgccgcg cgacgcgacg gatccggtcc
     3181 gcgtcggcga ggctggagcc cccgaatttc tggacgacga gcggcgcggc aggcatcgag
     3241 gcattctacc gctcgtgctg cgggtgcccg ggtggcggga tggttgcggg ccccgtcgtg
     3301 gcaccggtcc ggtcatcctg cgcgtccggc ggcctgctcg gcgccggatg tccgcggtgc
     3361 gggcgttaca aaggcggtcc cgtccgcgcg ccgccggatg tccgcggtgc gggcgttacg
     3421 gctcaccgga ccgggccgtc ccgctcggcg ccgcccgatg gacggcgctc cgccggactc
     3481 tctggcgcgt agcgcccgtg gtgtggtcat tcggcacatt cacaaagaac aggccgctcg
     3541 gtggcggggt cgtcggcgag cggagccagg ccacgcagtc tcgaccgcgg agcggaaatg
     3601 cggtcccgaa gatctccgcg tggcgcgtga ccctggaccg cgatgttgtg ctgtcggcga
     3661 tcacgaggaa tcgagcaacg ccgcgacaga cccagccccg ctcccgggcg atctccggtg
     3721 ccagtcgtgc cttccgatcg acgccgatga tcgtctggtt ggcgtcgccg atcgatgcct
     3781 tgacctcgaa ggtcgcgatc attctcgtca gcggatggcg accgacgaca tcgatcgagc
     3841 cgcgctccgc gtagatcgcg aacgacacct cgacctcggc catccacccg tagcgtcgga
     3901 gcagaccgac gagcacgtcg gcggtggcgg aatgccgctc gtcgaggagc cggtcaatcc
     3961 gctcaccctg ccagcggatg tccaggtcca gccgggcgcc gacggcttcg gcgaccgcga
     4021 cgaggtccgc ccaggcgatc cggagcagtt caccg
//
LOCUS       DATAIW010000372         8473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_54415, whole genome shotgun sequence.
ACCESSION   DATAIW010000372 DATAIW010000000
VERSION     DATAIW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54415"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="glgP"
                     /locus_tag="VHM48_11190"
     CDS             complement(<1..1059)
                     /gene="glgP"
                     /locus_tag="VHM48_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2756022.1"
                     /translation="MPTRVIIPTIPGALFRLPPQLEGLRALAYNLYWAWHPRTRVIWS
                     RIDRKVWTRHRNPIPVLAGPVDWSRLLDDTSFMAEVRDVLRDFNRYMANGSDRWYVRH
                     HAHELPGPVAYFCAEYGLYESLGIYSGGLGVLAGDHLKTTSDMSLPFVGIGLMYRRGY
                     FHQTIDADGHQEHAYPDYDLSRLPVGRVQGRDGEPLIVSVALPGRDLFIAVWAVQVGR
                     TPVLLLDTDIPENGDADRPITHILYVRGREMRLHQELVLGVGGVRVLRALGIEPAVWH
                     LNEGHSAFLLAERARELVAGGEDLDAAWQRVSRDSVFTIHTPVSAGNERFAVDLVRRV
                     AGPLLDGDGRPRTGGVPID"
     gene            1366..2847
                     /locus_tag="VHM48_11195"
     CDS             1366..2847
                     /locus_tag="VHM48_11195"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX2756023.1"
                     /translation="MPLRIAMIAAECEPWAKTGGLGDVVDALARALGHVPGATDGDVE
                     VFLPRYRSVPVPAAVAGSRVVRVPDPQAPDGVTEMTIVDVAANGYRLRLVDHPPAFGR
                     DGMYGPAAGGDFTDNAWRYGLFCRAALETLRSEPERPVDILHLHDWHSGPAAIQRDAW
                     AADDPILGRAAIVMTVHNLAYHGWTGRGDLGQLGLVPGGGVLPVDADGVDLIREGIVR
                     AEIANTVSPGFAREALTPAGSFGLADALAAKGDRFVGILNGLDTVVWDPATDVDLAAP
                     FSAGDLDGKAACRADLLERLGFDPADDGAVLGMIGRLDPQKGFDLIHDAAPRLLRAGA
                     RIVVQGSGDHYLAAGLRALAAEHPEGVALIERFDRAMARRIYAGADFFLMPSRFEPCG
                     TGQMISLRYGTPPVVRRTGGLADSVIDERTRPGEGTGFSFDDATPDALAKACEAAMAM
                     RSAGGAPWDSLIGRGMAVDFDWDRGSAPRYAALYERALAIRRS"
     gene            complement(2849..3751)
                     /locus_tag="VHM48_11200"
     CDS             complement(2849..3751)
                     /locus_tag="VHM48_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2756024.1"
                     /translation="MTVSTGLLAPPLTAGRADLPPTVVAALDDPVSPELGVVEAAGIP
                     FATRAWGDPTAAPILLIHGVTASSRIWWRVGPALAAGLERRVISVDQAGHGLTRQWTG
                     HHRFADNAADLVAFADAAGLARADLRVVGHSWGGMTAAALPGVGLVPEVLVLLDPPAI
                     PVASIATMLDDPVERRYDDLDEAIAALGRLHPTWPFGDVVAKAQALTQFDEPAVRAIL
                     TQNGNWDGGLAGLAAPAAAAVQIWLIRGEPSAGGLIPDEEANRIAERIGADHVITIAD
                     GAHAPMRLRPEAATLALLRALESA"
     gene            3831..4220
                     /locus_tag="VHM48_11205"
     CDS             3831..4220
                     /locus_tag="VHM48_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756025.1"
                     /translation="MANQFVVELKNEPGGLAILAEALAERGIDLRAIGGGGIGETGHI
                     IMTTADDDGARAVLETGNYTFIEGESILTEVDDRPGGMARLSRALADAGVNIYGHLFL
                     GRWGDRAMFAFVVDDPDKARPILEHKG"
     gene            4291..4629
                     /locus_tag="VHM48_11210"
     CDS             4291..4629
                     /locus_tag="VHM48_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756026.1"
                     /translation="MDATEDDALTTAADEIESLAGKWLAAEHELAAGSGNPGQSEINA
                     RALSERYDEAIRTASREDLRLAWEAARKIQGEQEMGSEAWANARRLSELLRREYQAAD
                     PETVADDPAD"
     gene            complement(4694..5707)
                     /locus_tag="VHM48_11215"
     CDS             complement(4694..5707)
                     /locus_tag="VHM48_11215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2756027.1"
                     /translation="MDARYSSLSPIADRGPLDSTWAHRQWYDPDAAGFNELTWVIEPP
                     PAAPSEARDVVDPQAILQALPAAALIVAFDGRIVAANGLADLLFGVRLTDRSIDDLVP
                     DGRRPLHALLRRAFSRTGETRSIGVHGALPARRADGSDFHVDLSLAAIQTAAGAAILV
                     IVVDTTAGAAPVDELARQALTDSLTGLGNRAALLAALTRRFGRPASERPLIVLAIDLD
                     GLREANRKHGHAAGDDLLRRYAARLTATVRTGDVVARTGGDEFAVLCSGSMAAGRAIA
                     ARLSGSAVERRSHPSTRVRLTASIGIAARRGHERSKTLLRRADAALLAAKAAGPHQVV
                     EAD"
     gene            6004..7410
                     /locus_tag="VHM48_11220"
     CDS             6004..7410
                     /locus_tag="VHM48_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2756028.1"
                     /translation="MHGNRLFLGLVSALAIALVACAAPAAPSWTYAPPAAGASASPNA
                     APAAMPMPGVSSAAPAGGAAIAAGGEIAIEAFDLGFKPAAVAVAAAGSYPVTFHNTGS
                     TLHDLTFADGTKLSADPGATVKGTVNVPASGLGFLCSIPGHAAAGMAGTIALAGAAPA
                     PAASGAPAAAGAALADPAAPAYVLRDAAAPALLAGTVHDVDLPIVEKDITVAKGFVVH
                     AWTFGGSVPGPTIRVHLGDTVRVHLTNNGTMSHSIDFHASQTAMNHQMVEIKPGATFT
                     YTFTADYAGVWMYHCGTAPALHHIANGMFGMVIVEPKGGLPKVDEEIALVQSEWYLGE
                     QKQPADYTKANAAAPAPDFVVFNGIANQYKDNPIVVTTKGRVRVFVLDAGPNIDSSFH
                     VVGTIFDTVIKEGVILAKGNAQGWGSQAVDLSPAQGAIIEFSPREDGMYPLVTHAFNF
                     VGKGAIGIFMAGDGDPLN"
     gene            7418..7861
                     /locus_tag="VHM48_11225"
     CDS             7418..7861
                     /locus_tag="VHM48_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2756029.1"
                     /translation="MRLELTKRADYAIRAVVALAAAAPDERLSVRRIAADQGIPVRFL
                     PQVMSDLGGAGLVEGIPGRTGGYRLARPAGRISLLEVIEAVEGDSHRETCVMRGGPCQ
                     LNGVCDVHRVFAATQEGMIRQLGAATIATLRKEDRKASSAAGLGG"
     gene            7858..8025
                     /locus_tag="VHM48_11230"
     CDS             7858..8025
                     /locus_tag="VHM48_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756030.1"
                     /translation="MNLVPSVMLSSSGVVLAAVAFYRRDDPAWRRGMVWIMGLAIMQV
                     VLGVVSALLIR"
     gene            8165..>8473
                     /locus_tag="VHM48_11235"
     CDS             8165..>8473
                     /locus_tag="VHM48_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756031.1"
                     /translation="MDVADELVVVRDGLRRIRAAEPEVAGVEAQAEERRVEPAISRAW
                     IDPIRPAPTTPIRTRPVITGSSPPRRRSSGDTVRDRGDPAAIRIGAGRARQPPDLRAA
                     R"
BASE COUNT         1239 a         2996 c         3069 g         1169 t
ORIGIN      
        1 atcgatcggg acgccgccgg tcctgggccg accgtcgccg tcgaggagcg ggccggcgac
       61 gcggcggacg agatcgactg caaaccgctc gttgccggcc gagaccgggg tgtggatcgt
      121 gaagacgctg tcgcgcgaga cgcgctgcca ggcggcgtcg aggtcctcgc cgccggccac
      181 gagctcgcgg gctcgctctg cgagcaggaa cgcggagtgg ccctcgttga ggtgccagac
      241 ggccggctcg atcccgaggg cacgcagcac gcgcaccccg ccgacgccga ggaccagctc
      301 ctggtggaga cgcatctcac ggccgcggac gtagaggatg tgggtgatcg gccggtcggc
      361 atcgccgttc tcggggatgt cggtgtcgag gaggaggacc ggcgtccgcc cgacctggac
      421 ggcccagacc gcgatgaaga ggtcgcggcc gggcagggcg acgctcacga tcagcggttc
      481 cccgtcgcga ccctggacgc gcccgaccgg gagccgggac aggtcgtagt cgggataggc
      541 gtgctcctgg tggccgtcgg cgtcgatcgt ctggtggaag tagccgcgcc ggtacatcag
      601 cccgatgccg acgaacggca gcgacatgtc cgaggtcgtc ttcaggtggt cgccggcgag
      661 gacgccgagg ccgccggagt agatcccgag cgattcgtac aggccgtact cggcgcagaa
      721 gtaggcgacg ggcccgggca gctcatgggc gtggtgacgg acgtaccagc ggtcggatcc
      781 gttcgccatg tagcggttga agtcgcggag gacgtcgcga acctcggcca tgaagctcgt
      841 gtcgtcgagc aggcggctcc agtcgaccgg gccggccagg acggggatcg ggttgcggtg
      901 gcgggtccag accttccgat cgatccggct ccagatcacg cgggtccgcg gatgccacgc
      961 ccagtacagg ttgtaggcga gggcgcggag cccctcgagc tggggaggga ggcggaacaa
     1021 ggcgcccgga atcgtcggaa tgatgacgcg cgtcggcatg agtctggatg atagtcgctg
     1081 gcagggtcgg tgtgaaccgg tttacgccgc cccgcccggt ccccggagtc cgcgcgcctc
     1141 tcgtctgatc tcgcggggga tcattcccgt ctggatgaac gtggccgggg tcgcgattgg
     1201 gcgggaaccg gtggcaccac gggagggggc cgtggtcact cgccaccagg tgatctccgg
     1261 ggagatcacg gcgcgctgcg gtgccgcgcg ggaaccggcc cgggagcgcc agccaggcac
     1321 cccagccacg cagtaggcaa cgccgcagcc gattacgatg ggccgatgcc gctccggatc
     1381 gcgatgatcg ccgccgaatg cgagccgtgg gccaagaccg gcggcctagg cgacgtggtc
     1441 gatgccctcg cccgggcgct cggccacgtg cccggcgcga ccgacgggga cgtcgaggtc
     1501 ttcctgccgc gttaccggtc ggtgccggtg ccggccgcgg tcgcgggctc gcgcgtcgtc
     1561 cgcgtcccgg acccccaggc acccgacggg gtcaccgaaa tgacgatcgt cgacgtcgcc
     1621 gcgaacggct accggctgcg cctcgtcgat cacccgccgg cgttcggtcg cgacgggatg
     1681 tacgggccgg cggccggcgg cgacttcact gacaacgcct ggcgctacgg cctcttctgt
     1741 cgggccgccc tcgaaaccct ccggtcggag cccgagcggc cggtcgacat cctccatctc
     1801 cacgactggc acagcggtcc ggctgcgatc cagcgcgatg cgtgggcagc ggacgatccg
     1861 atcctcggtc gggcggcgat cgtgatgacc gtccacaacc tggcgtacca cggctggacc
     1921 gggcgcggcg atctcggcca gctggggctg gtcccgggtg gcggggtcct gcccgtcgat
     1981 gccgatggcg tcgatctcat ccgcgagggg atcgtccgag cggagatcgc caacaccgtc
     2041 tcgcccgggt tcgcccgcga ggcgctgaca cccgcaggga gcttcgggct ggccgatgcg
     2101 ctcgcggcca agggcgatcg gttcgtcggg atcctcaacg gcctcgacac ggtcgtctgg
     2161 gatcccgcga ccgacgtcga cctggctgcg ccgttctcgg ccggtgatct cgacggcaag
     2221 gcagcctgcc gggccgacct gctggagcgg ctcgggttcg atccggccga cgacggcgcc
     2281 gtgctcggga tgatcggccg gctcgatccc cagaagggct tcgacctgat ccacgacgcc
     2341 gcgccgcggc tgcttcgcgc cggcgcccgg atcgtcgtcc aggggtccgg cgaccattac
     2401 ctcgcggccg ggttgcgggc cctcgcggcc gagcatcccg agggtgtcgc gctgatcgag
     2461 cggttcgacc gggcgatggc ccggcggatc tacgccggag cggatttctt tctcatgccg
     2521 tcgcgcttcg agccatgcgg gaccggccag atgatctcac tccggtacgg gacgccgccg
     2581 gtcgttcgcc ggacgggcgg tctggccgac agcgtgatcg acgaacggac gcggccgggg
     2641 gagggaaccg ggttctcctt cgacgacgcc acgccggatg cgctggcgaa ggcctgcgag
     2701 gcggcgatgg cgatgcggtc ggccggtggg gcgccctggg actcgctcat cgggcgcggg
     2761 atggccgtcg acttcgactg ggatcggggc tcggcgccgc gctacgccgc cctgtacgag
     2821 cgagccctcg cgatccgccg cagctgagtc aggccgactc gagcgctcgc agcagggcca
     2881 gggtggccgc ctccggccgc aatcgcatcg gagcgtgggc accgtcggcg atcgtgatga
     2941 cgtgatcggc gccgatccgt tcggcgatcc ggttcgcctc ctcgtccggg atcaggccac
     3001 cggccgaggg ctcgccgcgg atgagccaga tctggactgc cgctgcggct ggagcggcga
     3061 ggccggccag cccgccgtcc cagttgccgt tctgggtgag gatcgcccgg acggccggct
     3121 cgtcgaactg ggtcagggcc tgggccttgg cgacgacatc gccgaacggc caggtcgggt
     3181 gcaggcgtcc gagcgcggcg atcgcctcgt cgaggtcgtc gtagcggcgc tcgaccgggt
     3241 cgtcgagcat cgtcgcgatc gacgcgaccg ggatcgccgg tgggtcgaga aggaccagga
     3301 cctccgggac caggccgacg ccaggcagcg cggccgcggt catcccgccc cagctgtggc
     3361 cgacgacccg gaggtctgcc cgggcaagtc ccgcggcgtc cgcgaacgcg acgaggtcgg
     3421 cggcgttgtc ggcgaagcga tgatggccgg tccactgccg ggtgagccca tggcccgcct
     3481 ggtcgaccga gatgacccgg cgctccagcc cggccgcgag cgccgggccg acccgccacc
     3541 agatccgcga cgacgccgtg acgccatgga tcagcaggat cggcgccgcc gtcgggtcgc
     3601 cccaggcgcg cgtcgcgaac gggatcccgg ccgcctcgac gaccccgagc tcgggtgaaa
     3661 ccggatcgtc gagcgcggca accacggtcg gcggcaggtc ggctcgaccg gcggtcagcg
     3721 gcggagccag gaggccagtc gagacggtca ccgcccgacc gtatcacgtc accccgcggt
     3781 tcgagccagc ggctagaatc gccgctcagc ccagatcgcc ggaggtcccc atggccaacc
     3841 agttcgtcgt cgagctgaag aacgaacccg gcggtcttgc catccttgcc gaggctctcg
     3901 ccgagcgtgg catcgatctg cgggcgatcg gcggcggcgg gatcggcgag accggccaca
     3961 tcatcatgac cacggccgac gacgacggcg cgcgggctgt cctcgagacc ggcaactaca
     4021 cgttcatcga gggcgagtcg atcctgaccg aggtcgacga tcgacccggc ggcatggccc
     4081 gcctgtcgcg cgcccttgcc gatgccgggg tcaacatcta cggccacctc ttcctcggcc
     4141 gctggggcga tcgggcgatg ttcgccttcg tcgtcgacga cccggacaag gcccgcccga
     4201 tcctcgaaca caagggctga ccgccgtcag gcgcgtggcc gcccggctcg tggccggcgg
     4261 tgatcagcct cgatcggcta ccgttgggtc gtggacgcga ccgaggacga cgccctgacg
     4321 accgccgccg acgagatcga gagcctcgcc ggcaagtggc tggccgccga gcacgagctc
     4381 gccgccggaa gcggcaaccc gggtcagagc gagatcaacg cccgggccct cagcgagcgc
     4441 tacgacgagg cgatccggac cgccagccgc gaggacctcc gacttgcctg ggaagccgcc
     4501 cgcaagatcc agggcgagca ggagatgggc tcggaggctt gggcgaacgc ccgacgcctc
     4561 tcggagctgc tccgtcgcga gtaccaggcc gcggacccgg agaccgtcgc ggacgacccc
     4621 gccgactgac ccgcgttcgg cacccacccg cgaccggagc gcgatccggt ccgtcgcccg
     4681 ggctcccgga cgctcagtcc gcctcgacga cctggtgggg gccggcggcc ttcgcggcca
     4741 ggagggccgc gtccgctcgc cgcagcaacg tcttcgatcg ctcgtgtccg cggcgggccg
     4801 cgatgccgat cgacgcggtc aggcggaccc tcgtcgacgg atgggaccgg cgctcgactg
     4861 ccgatccgga cagcctggcc gcgatggccc ggccggcggc catcgacccg ctgcacagga
     4921 cggcgaactc gtccccgccg gtccgggcca cgacgtcgcc ggtgcggacc gttgcggtca
     4981 ggcgcgcagc atagcggcgc agcaggtcgt ctcccgccgc gtgaccgtgc ttccggttcg
     5041 cctcgcgcag gccatcgaga tcgatggcga gcacgatgag cggtcgctcc gacgcgggtc
     5101 ggccgaaccg ccgggtcagt gccgccagca gcgccgcccg attgccgagt cccgtgagcg
     5161 agtcggtcag cgcctggcgc gccagctcgt cgacgggggc ggccccggcg gtcgtgtcga
     5221 cgacgatcac caggattgcg gcgcccgcgg cggtctggat ggcggcgagg ctgaggtcga
     5281 cgtgaaagtc ggagccgtcg gctcgccgtg ccggcagggc tccgtggacg ccgatcgatc
     5341 gggtttcgcc cgttcgcgag aaggctcgac gaaggagagc gtggagcggc cgccgcccat
     5401 ccggcacgag gtcgtcgatg ctccggtcgg tgagccgaac tccgaacagg agatccgcga
     5461 gcccgttcgc ggccacgatc cggccatcga acgcgacgat cagggcggcc gccgggaggg
     5521 cctgcagaat cgcctgtgga tcgacgacgt cgcgcgcctc cgacggcgcg gccgggggcg
     5581 gttcgatcac ccacgtcagc tcgttgaacc cggcggcatc ggggtcgtac cactgtctat
     5641 gggcccaagt cgaatccagc ggtcctcgat ccgcgatcgg cgacagggag ctgtatcgcg
     5701 cgtccatccg agcctcctca acgcgccgat gtgcccccca gaagcgcgtc cccaccggag
     5761 agggcagctt cgcgtccgag gacgcgggaa catgcccgga cccggaatcg tacgcgcgac
     5821 ggaccgatcg cgccagtgcc ggcgggacgt gcgaacgtga agcgacccgg tcccgaacgg
     5881 gcgacggccg caagaagggg ccatttggca cgggtgtgtg cacgcgatcc tgggcgcaaa
     5941 ctcacactaa atcagtgtga gttttgcagg agccccgtcg atgctcgggg ccaggagatc
     6001 gagatgcacg gcaaccgact gttccttggc ctcgtgagcg ctcttgcgat cgcgctcgtc
     6061 gcctgtgccg ccccggctgc cccgagctgg acgtacgccc cgcccgccgc cggcgcgtcg
     6121 gcatcgccga acgcggcccc tgcggcgatg ccgatgcccg gggtgagcag cgcggcaccg
     6181 gccggcggcg cggcgatcgc ggccggtggc gagatcgcca tcgaggcgtt cgacctcggc
     6241 ttcaagcccg ccgccgtcgc ggtcgcggcc gccggatcct atccggtcac cttccacaac
     6301 acgggttcca ccctccacga cctgacgttc gccgacggca ccaagctgtc ggccgacccc
     6361 ggcgccaccg tcaagggcac ggtcaacgtt ccggcgtccg ggctcggctt tctctgctcg
     6421 atccccggcc acgcggcagc cgggatggcc gggaccatcg cgctggccgg cgccgcgccg
     6481 gcaccggccg cgtccggcgc acccgccgcc gccggcgcag ccctggctga cccggccgcg
     6541 cccgcctacg tcctgcgcga tgccgcggcc ccggccctcc tggccggcac ggtccacgac
     6601 gtcgacctgc cgatcgtcga gaaggacatc accgtcgcga aggggttcgt cgtccacgcc
     6661 tggacgttcg gtgggagcgt tcccggtcca acgatccggg tccacctcgg tgacaccgtc
     6721 cgcgtccacc tgacgaacaa cggcacgatg tcgcactcga tcgacttcca cgcaagccag
     6781 accgccatga accaccagat ggtcgagatc aagccgggag caacgttcac gtacaccttc
     6841 accgccgact acgccggggt ctggatgtac cactgcggca cggcgccggc actccaccac
     6901 atcgccaacg gcatgttcgg gatggtcatc gtcgagccga agggcggctt gccgaaggtc
     6961 gacgaggaga tcgccctcgt ccagagcgag tggtatctcg gcgagcagaa gcagccggcc
     7021 gactacacga aggcgaacgc cgccgcgccg gcgcccgact tcgtcgtctt caacggcatc
     7081 gccaaccagt acaaggacaa cccgatcgtg gtcacgacca agggtcgggt ccgcgtgttc
     7141 gtcctcgatg ccggcccgaa catcgacagc tcgttccacg tcgtcgggac gatcttcgac
     7201 acggtcatca aggaaggcgt gatcctcgcc aagggcaacg cccagggctg gggcagccag
     7261 gccgtggatc tctcgccggc ccagggcgcg atcatcgagt tcagcccccg ggaggacggg
     7321 atgtacccgc tcgtgaccca cgccttcaac ttcgtcggca agggcgcgat cgggatcttc
     7381 atggccggcg acggcgaccc gttgaactga gccggacatg cggctcgagc tcaccaagcg
     7441 cgcggactac gcgatccggg ctgtcgtcgc actcgccgcg gccgccccgg acgaacgcct
     7501 gagcgtgcgt cggatcgccg ccgatcaggg tatcccggtc cgtttcctgc cccaggtcat
     7561 gtccgacctg gggggagcgg ggctcgtcga aggcattccc gggcgaacgg gcggctaccg
     7621 tctcgcccgc ccggccggca ggatctcgct cctcgaggtc atcgaggcgg tcgagggcga
     7681 cagtcaccgc gagacctgcg tcatgcgcgg cggaccgtgc cagctgaacg gagtctgcga
     7741 cgtgcatcgc gtcttcgcgg ccacccagga gggcatgatc cgccagctcg gcgcggccac
     7801 gatcgcgacc ctgcggaaag aggaccggaa agcgtcatcg gcggctggac tcggcgggtg
     7861 aacctcgttc catcggtcat gttgtcgagc tccggcgtgg tgctcgccgc cgtggccttc
     7921 taccgccgcg acgatcctgc gtggcggcgc ggaatggtct ggatcatggg gctggccatc
     7981 atgcaggtcg tgctcggagt cgtgtcggcc ctgctgatcc gctgaatgag gcggcggctt
     8041 cggacgggct gccgaccgcg ggtcagaggc cgagctggcc gaagacgccc cggctcaacg
     8101 cctcctcgag gcccttcccc tcggccttga cgtcgtccat caccttcgtc aggcagtcct
     8161 tgatgtggac gtcgcggatg agctcgtggt agtccgcgac ggcctgcgtc ggatccgcgc
     8221 cgccgaaccg gaagtggccg gcgtcgaggc acaggccgag gagagacggg tcgagccggc
     8281 gatcagccgg gcgtggatcg accccatccg gccggcgccg accacgccga tccgcacgcg
     8341 cccggtcatc accggatcct cgccgccgcg acgtcgctca tcgggcgata cggtacgcga
     8401 tcgcggcgat ccggccgcga ttcgcattgg agcggggcga gctcgtcagc cgcccgactt
     8461 gagagcggcg agg
//
LOCUS       DATAIW010000373         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_54442, whole genome shotgun sequence.
ACCESSION   DATAIW010000373 DATAIW010000000
VERSION     DATAIW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54442"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="ccsA"
                     /locus_tag="VHM48_11240"
     CDS             <1..532
                     /gene="ccsA"
                     /locus_tag="VHM48_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2756032.1"
                     /translation="SIGFIPTGVALALLLYAASLPSNISQLVPALQNPPLITIHVGLA
                     VLSYGIFATSFAAGVGYLLQGQHDRFAWLPSHKVLDEVAYRAVITGFPIFATMIILGS
                     WWASIAWSRYWGWDPKETAALITWLSYAVYLHARNQKSWAGRPAALLLVVAFGMVIVT
                     YSGSLWFNGLHSYSGL"
     gene            645..1151
                     /locus_tag="VHM48_11245"
     CDS             645..1151
                     /locus_tag="VHM48_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756033.1"
                     /translation="MLRLEGRKRIEQAVDWTARHHHVGPFAVGLAATLAVLLPIPVGG
                     SVFTLAADNPVVGPLTGILGVVLSCTVYPMYAGTAVAGYVVGRSSTDRRAWRLAVGGS
                     LIPIAIPLLLLWYEALRNYGGSSLVLPVMIPLLYIPFQLGHRRGLQTAQRLPAPAVEL
                     VSVPEPTN"
     gene            complement(1896..2741)
                     /locus_tag="VHM48_11250"
     CDS             complement(1896..2741)
                     /locus_tag="VHM48_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756034.1"
                     /translation="MPVSMTENLHAEILSVAPLQTAERLAALGRTLDAVPELAPEKMG
                     PRDPPRTRITSVEQQLVEWAPKIMPGDYYNQFMVRAKPHASGLLWVTGDIWAFPPIAP
                     HQLDLDVDETWFAEPDRAARLEVFADLFRRACESMDAFWGGAELTSLRRQRNDFVRAA
                     QVAGTLALPTLGSGWDVREHALWDIPWLLYLGPAFVARWGDRLSALGVRRDPTANGGL
                     VIWSTPHPFVFEPLAREMLDYAWKRPFHEALGDGAIVHESWRDPGRGVVVPTYEDHRR
                     ATAGG"
     gene            complement(2855..>3134)
                     /locus_tag="VHM48_11255"
     CDS             complement(2855..>3134)
                     /locus_tag="VHM48_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756035.1"
                     /translation="AAPVAASIGRPSRVAPFVSGRSASAFFDATPPAAWPAATVCGCI
                     SFSTAATTRGGATAAGQLASSVVVSGAVIAVGVAAGALAARLLGRDGR"
BASE COUNT          449 a         1123 c         1104 g          458 t
ORIGIN      
        1 ctcgatcggc ttcatcccga ccggcgtcgc gctggccctg ctcctctacg ccgcgagcct
       61 gccatcgaac atcagccagc tcgtgccggc cctccagaac ccgcccctga tcacgatcca
      121 cgtcgggctg gcggtgctgt cgtacggcat cttcgcgacg tcgttcgccg ccggcgtcgg
      181 ctacctgctc cagggccagc acgatcggtt cgcctggctg ccgagccaca aggtcctcga
      241 cgaggtcgcc tatcgcgcgg tgatcacggg cttcccgatc ttcgccacga tgatcattct
      301 cggctcgtgg tgggcctcga tcgcctggtc gcgctactgg ggctgggatc cgaaggagac
      361 cgcggccctg atcacgtggc tgtcgtacgc ggtctacctc cacgcccgca accagaagtc
      421 gtgggccggc cggccggccg cgctcctgct ggtcgtcgcg ttcgggatgg tgatcgtcac
      481 ctactcgggc tcgctctggt tcaacggcct gcactcctac agcgggctgt agatcgacgt
      541 ccagactccg acacgcctcg ccgtcacgca tagggcgtca aggcgtctcc gaggtacggc
      601 cacaggcttc ccgccttcat gcagggccga agcaggagcg cgcgatgctc agacttgaag
      661 ggcgcaaacg cattgagcag gccgtcgatt ggacggcacg ccaccatcac gtgggcccgt
      721 ttgccgtcgg actggccgcg accttggccg tgctcctacc gattccggtc gggggcagcg
      781 tcttcacgct cgccgccgac aacccagtgg taggacccct gactggcatc ctgggcgtcg
      841 tcctgagctg caccgtctat ccgatgtacg ccgggaccgc cgtcgccggg tatgtcgtcg
      901 ggcggagcag cactgatcgc agggcctggc ggctggctgt cggcggctct ctgatcccga
      961 tcgcgatccc gctgctcttg ctgtggtacg aggcgttgcg aaactacggc ggaagcagcc
     1021 tcgtgctccc ggtcatgatt ccgttgctct atatcccgtt ccagctcggt catcgacgag
     1081 gcctccagac tgcccaacga ctgccagcgc cggctgtcga gctggtctcg gttccggaac
     1141 ccacgaactg acgcccacga ctgggcgtcg ccgctcgtct actgcgcggg cggcctggcc
     1201 gatccatgag tctgggcgcg ccgtgccccc gccgcccgga cgcccggaat cgaacgagcc
     1261 ccgaccgatc ggccgagacc cgcagcgcga ggcgagatcg cactgccgcc acccgagacg
     1321 gatcctgatt cgccggacca tcgcgccgaa ccgcaagtca tccatgcctg agtctggccc
     1381 ggcggtcttg tctcgcacgt atcggaggcc ttcgagtggg tggccggctc cccgttcaca
     1441 agccgaccgc agttggccaa atggtcatcc ccggaccgta agggctggtc cgcgggcctc
     1501 gagggccggc ccgatcgccc ggtcacgcac agtcgagcct cggcgctggc gtcctggctc
     1561 ggaatcggag ggtcaaccgg tcctaacggt ccgtggatgg cagtcgggcc agccgaatag
     1621 cgtcatggct cggtgggggc ctcgagggat ccggtgcggc ccggcagggt caggcgggct
     1681 cgatgggact cgggcgcggc tcgccggggc ctcgacgggg cctcgacggg gcctcggcac
     1741 ggctcaccaa ggctcggcgg cgggcggcgg cgggcggcgg cgcccaaccg ctacgcctcg
     1801 ccgatggccg acggaagtat tcggctattc gaaggggcag cggcccctct cgtccggcga
     1861 ccgcccgaac gccagggcgt cagggcgtca gggcgtcagc cgccggccgt cgcccggcgg
     1921 tgatcctcgt acgtcggcac gacgaccccg cggcccggat cgcgccacga ctcgtggacg
     1981 atcgcgccgt cgcccagagc ctcgtggaac ggccgcttcc aggcgtagtc gagcatctcc
     2041 cgcgcgagcg gctcgaagac gaacggatgc ggggtcgacc agatgacgag cccgccgttg
     2101 gccgtcggat cgcggcggac gccgagcgcc gacagccgat cgccccagcg cgccacgaac
     2161 gccggcccga gatagagcag ccacgggatg tcccagaggg cgtgttcgcg cacgtcccac
     2221 ccgctcccga gtgtcgggag agcgagcgtc ccggcgacct gcgccgcgcg cacgaagtcg
     2281 ttccgctgcc gccggaggga ggtcagctcg gcgccgcccc agaacgcgtc catcgattcg
     2341 caggcgcgcc ggaagaggtc ggcgaagacc tcgagacggg ccgcccggtc gggctcggcg
     2401 aaccacgtct cgtcgacgtc gagatccagc tggtgcggtg cgatcggtgg aaacgcccag
     2461 atgtcgccgg tgacccagag caggccgctg gcatgtggct tcgcgcggac catgaactgg
     2521 ttgtagtagt cgccgggcat gatcttcggc gcccactcga cgagctgctg ctcgaccgag
     2581 gtgatccggg tccggggcgg atcccgcgga cccatcttct ccggggcgag ctccgggacg
     2641 gcgtcgagcg tccggccgag ggcggccaga cgctcggccg tctggagcgg cgcgacggac
     2701 aggatctcgg cgtgaaggtt ctcggtcatc gagacgggca cgcgtcgatt ctgacggatc
     2761 gcccggcccg tgtcggccgc cagcgcaatc ctcggacgtc cgcgagcgcg tcatgacccg
     2821 gcgcgcgtct cgcgaccgcc ccgcgcgcaa cccgtcagcg gccgtcgcgg ccgaggaggc
     2881 gggcggcgag cgcgccggcg gcgactccga cggcgatcac ggcgccgctg acgacgaccg
     2941 aagaggccag ttgcccggcc gcggtggcac cgccgcgggt cgtcgcggcc gtgctgaagg
     3001 agatgcagcc gcacacagtg gccgcgggcc aggcggccgg cggcgtcgcg tcgaagaacg
     3061 ccgatgccga gcggcccgac acgaagggcg caaccctcga ggggcgcccg atcgaggcgg
     3121 caaccggcgc ggcg
//
LOCUS       DATAIW010000374         6553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_173758, whole genome shotgun sequence.
ACCESSION   DATAIW010000374 DATAIW010000000
VERSION     DATAIW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173758"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1797
                     /locus_tag="VHM48_11260"
     CDS             418..1797
                     /locus_tag="VHM48_11260"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEX2756036.1"
                     /translation="MTSSSTRSFTVDRALVRELKNHEDAVFRERHPRSIALRERGRAS
                     MPNGVPMAWHASSYHDLAPWAAEGHGARFTDVDGVTYSDFNIADLSMFCGYAPDPLVR
                     AVSDRIARGNQFLLPTEDAIVVSEELARRYGLPKWQYTSAASQANTEAIRVARVATGR
                     DKVLLFDGHYLGHFDQALVELDDDGRLVAEELGVPATVTEGTIVVPLNDVEALDRALE
                     TRQIALVLTEPALTNNFGLILPDDGWHDALRTLTREHGTLLAIDETHTQVVGPGGLTR
                     QWGLEPDFVTCGKSIAGGVPFGAWGMTDEIADVVRQAKGPDGERSDLVAIGGTLFGNA
                     LAMAAVRAVLTEILTPAAYASTQRLGARLADGMRASVAAAGLPWHIHHLGPRAGYTYR
                     DTAVRNAAEARAVQDDLLTRLVRVWLANRGVWEAIVGAGPVCSVPATDADVDGYLAGW
                     ATLIERLSR"
     gene            complement(1787..3088)
                     /locus_tag="VHM48_11265"
     CDS             complement(1787..3088)
                     /locus_tag="VHM48_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756037.1"
                     /translation="MAPSPPPARSTDSRPRPLFALREFRALLGARSTNALAVSALATV
                     VAFQTYDITRDPLALGWLGLVEAIPALSLVLLGGHLADRRDRRTIVVISSGIVTLCAI
                     VLAALSAAGALTLVGILAVIFATGVASGFERPALTALEAQVVPRDEAARGISMLSSVS
                     QGGGILGPAVGGIAIVLVGIPLTYAGIAVLLAISTACLAVIARKPIPARVEGESMIRS
                     LLGGIRYVARTPALVGSMALDLFAVFFGGAIALLPIFATDILQVGAVGLGLLRTAPSV
                     GALLVMLVATRRPPSRHAGRMLLLCVAGFGVSMIIFGASTIFAVSLAALFFAGITDGI
                     SMIIRSTILRVLSPEQIRGRVASVNWVFIGASNELGAFESGVAARLFGTVPSVIGGGI
                     LTLAVVGVTAWLVPSLRSLDIDRAAPGDAGTAGPGSPAVSG"
     gene            3262..4206
                     /locus_tag="VHM48_11270"
     CDS             3262..4206
                     /locus_tag="VHM48_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756038.1"
                     /translation="MSARLTGFAALAASVVIVVGCGSSPAASSASPASTPGSSAPASA
                     PRSGPPSASPSASPSAATATVLLKVTSEGGFINPSANLNALPIVEVLSDGRILTVGPV
                     DAIAPAPLLPTVQVRATGAAGAAAILAAIRQAGLDQRSTAGPGIPGDSGTDIFSVTVD
                     GETTETRLAGAGLGVGGPGGPGLGGSADPGKAAAFDLLNKLLDPAETWGGGAIQPSSY
                     APTGYRVFVIPGAPSVDPATPQTPVAWPLSTALDAFGTAAAPDRGIAGLRQGAVLGPD
                     AVTLGPILARATVETAFTSGGKSFTLAVRPLLPDELGG"
     gene            4444..5604
                     /locus_tag="VHM48_11275"
     CDS             4444..5604
                     /locus_tag="VHM48_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2756039.1"
                     /translation="MFDEDVEPVRQPRRHRPGPELGGAVGTVHALLPVRFFFGATFLY
                     AGLDKLIDPRFLDPSSPASIQAQMIIFIRASPIAALVRLGQPLAVPIGLAIAIAEIAI
                     GLGALTGLAYRAAAWGGAILSLIFFLTASWATRPYYYGPDLPYAIGWVALASAGHGGL
                     FVSRRLLAWAGGRGSEHALDPRGRGSRRPLRGVPAEADATDSLPLRDRRTVLQAGVLG
                     FFALIAASVAVPFRISRAGGAPADGGPDASAGVAGGSPGASSGPGRSSAPQPSAALGG
                     LAVARVADLAKTGARAFTVPFSAPAPFPAGDPAVIVKLADGSFVAFDAVCTHEGCTVQ
                     WDRVDRVLLCPCHSAAFDPAHDAAVLQGPTNQPLASIPIVVDAASGTISLRA"
     gene            complement(5662..5997)
                     /locus_tag="VHM48_11280"
     CDS             complement(5662..5997)
                     /locus_tag="VHM48_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756040.1"
                     /translation="MVRRHRRFELLQVTLDETPIGSCTIDAWEDDDGHEQWTARVLMK
                     TGHGSTEGVLHGSTRAGEPLAGQVRFAADQQGPRGGRTVLVELHGQGPLEPAPPIEVA
                     ATTDHPPAT"
     gene            complement(6159..>6553)
                     /locus_tag="VHM48_11285"
     CDS             complement(6159..>6553)
                     /locus_tag="VHM48_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2756041.1"
                     /translation="PAPTHPGSTMTITRLDPTHPLWLGGPTLTVIDEETGHPLSLPVA
                     TVELDGAHYLVSPDGETDWVRNLRAAGEGVLRRGRTGEPVEAKEISGGEHDRVVTAYR
                     DQMGERTSEPFEALPNPADHPVFRIEPR"
BASE COUNT          884 a         2414 c         2344 g          911 t
ORIGIN      
        1 atctcgaagc cgtgctcggc gaggacgcgc ttcgcctcgt cgacgatccg cccgatcagc
       61 tcgggctcga ggatgttcag gccgggtcgc atcgccgctc catcatgtcc gaccgatggt
      121 cggggctgac acgccgaaaa cccggccagt tgcacgccgc ggatcgttgc ggctcgtgca
      181 tgccgcccac gcagcagcga cggcccctgt cagaacgacg ttttcggtgc gtccgggcac
      241 cgcaggcgtc ggaatccgca cctcaccgga ttgccccgct ccgccccgaa ctcgcgcaaa
      301 gacgatcgcc gggccctcga gcccggcgat cccgtgtcgt gcgcgacggt ctgcctcgtc
      361 ggccaccatc gcaacatccg gccaccgttg tggcgacgct cgctacactc ccgcccgatg
      421 acctcctcct cgacgcgatc gttcaccgtc gatcgggcgc tcgttcgcga gctgaagaac
      481 catgaggatg ccgtctttcg cgagcgccac ccgcgctcga tcgcgctccg cgaacggggc
      541 cgggcatcga tgcccaacgg cgttcccatg gcctggcacg cgagctccta ccacgacctg
      601 gcgccgtggg cggcggaggg gcacggcgcc cggttcaccg acgtcgacgg cgtcacgtac
      661 agcgatttca atatcgccga cttgtcgatg ttctgcggtt acgcccctga ccccctcgtc
      721 cgggccgtct ccgatcggat cgcgcggggg aaccagttcc tccttccgac cgaggacgcg
      781 atcgtcgtgt cggaggagct ggcccgtcgc tacgggctgc cgaaatggca gtacacctcg
      841 gcggccagcc aggcgaacac cgaggcgatc cgggtcgccc gggtggcgac cggtcgcgac
      901 aaggtcctgc tcttcgacgg ccactacctg ggtcacttcg accaggccct cgtcgagctc
      961 gacgacgacg gtcgcctggt ggcggaggag ctcggcgtcc ccgccacggt gacagagggc
     1021 acgatcgtcg tcccgctcaa cgacgtcgag gcgctcgacc gggcgctcga gacgcgccag
     1081 atcgcgctcg tcctgaccga gcccgcgctc acgaacaact tcgggctgat cctgcccgat
     1141 gacggctggc acgacgccct ccggaccctg acccgcgagc acgggacgct gctcgcgatc
     1201 gacgagaccc acacccaggt cgttggtccg ggcgggctga cccgccagtg ggggctcgag
     1261 ccggacttcg tcacttgcgg caagtccatc gccggcggcg tgccgttcgg ggcctggggc
     1321 atgaccgacg agatcgccga cgtcgtgcgc caggcgaagg gccccgatgg cgagcgatcg
     1381 gacctggtcg cgatcggcgg gacgttgttc ggcaacgccc tcgccatggc cgcggtccgg
     1441 gccgtcctga ccgagatcct cacgccggcg gcctatgcct cgacccagcg gctcggcgcc
     1501 cgcctcgccg acgggatgcg ggcgtcggtt gcggccgccg gtctgccctg gcacatccat
     1561 catctcggac cgcgagccgg ctacacgtat cgcgacaccg cggtccgcaa cgccgccgag
     1621 gcgcgggcgg tccaggacga cctgctgacc aggctcgtcc gcgtgtggct ggccaaccgc
     1681 ggggtctggg aggcgatcgt cggcgccgga ccggtctgct ccgtgccggc gaccgatgcc
     1741 gacgtcgacg gctatctcgc cggctgggcc acgctgatcg agcgcctcag ccgctgacgg
     1801 cgggcgaccc cgggcccgcc gtcccggcgt cgccgggcgc cgcgcggtcg atgtcgagtg
     1861 accgcagcga cggcaccagc caggccgtca cgccgaccac cgcgagcgtg aggatcccgc
     1921 cgccgatcac gctcgggacg gtcccgaaga ggcgcgccgc gacgccgctc tcgaacgccc
     1981 cgagctcgtt cgacgcgccg atgaagaccc agttgaccga tgcgacccgg ccccggatct
     2041 gctccggcga gaggacccgc aggatcgtgc tgcggatgat catgctgatc ccgtcggtga
     2101 tcccggcgaa gaacagcgcg gcgagtgaga ccgcgaagat cgtcgatgcg ccgaagatga
     2161 tcatgctgac gccgaagccc gcgacgcaca ggaggagcat ccgtccggca tggcggctcg
     2221 gcggccggcg cgtcgcgacg agcatgacca gcagcgcccc gaccgagggc gccgtccgca
     2281 gcaatcccag cccgaccgcg ccgacctgga ggatatcggt cgcgaagatc ggcagcaggg
     2341 cgatcgcccc gccgaagaag accgcgaaca ggtcgagcgc catcgagccg acgagggccg
     2401 gcgtccgggc cacgtagcgg attccgccga ggaggctccg gatcatcgac tcgccctcga
     2461 cccgggccgg gatcggcttg cgggcgatca ccgccaggca ggccgtcgag atcgccagca
     2521 ggaccgcgat gcccgcgtac gtcagcggga tgccgacgag cacgatcgcg atgccaccca
     2581 cggcgggccc gaggatcccg ccgccctggc tgacgctgct cagcatcgag atgcctcggg
     2641 ccgcctcgtc gcgcggcacg acctgcgcct ccaatgccgt cagtgccggg cgctcgaagc
     2701 cgctcgcgac accggtcgcg aagatcaccg cgaggatgcc gaccaaggtc agcgcgcccg
     2761 ccgccgacag ggcggcgagg acgatcgcac acagggtcac gatcccgctc gagatgacga
     2821 cgatcgtccg ccggtcgcgg cggtcagcga ggtggccccc gagcaggacc agggacaggg
     2881 cggggatggc ctcgacgagc ccgagccagc cgagcgccag cgggtcgcgg gtgatgtcgt
     2941 aggtctggaa ggcgacgacg gtcgcgaggg cgctgaccgc gagggcgttg gtcgagcgcg
     3001 cgccgagcaa ggcgcggaat tcgcggagcg cgaacaacgg gcgcggacgg ctgtccgtgg
     3061 agcgcgcggg cggtggggag ggagccaccg gccgatggta cgcaacgccg ccgatctgcc
     3121 tcgctcaccg gtccggctcc cccaccggtc cggctcggct gccggcccgg ccgggaccgg
     3181 cggaaccgga ggccgcagcg cggcgtctgc ccgcggaacg cggcgccgcc gcgagccaag
     3241 caccagagtt gtggggtctc gatgtccgct cgtctcacgg ggttcgcggc gctcgccgcg
     3301 tcggtcgtca tcgtcgtcgg ctgcgggagc agccccgccg cgagcagcgc atcgcccgcg
     3361 tccacgcccg gctcgtcggc cccggcgtcg gcgccacgat ccggcccgcc gtcggcatca
     3421 ccgtcggcat caccgtcggc cgcgacggcc acggtcctgc tcaaggtgac gtcggaaggt
     3481 gggttcatca acccgtcggc gaacctgaat gcgctgccga tcgtcgaggt gctgagcgac
     3541 ggccggatcc tgaccgtggg ccctgtcgat gcgatcgcac cggcgccgct cctgccgacc
     3601 gtccaggtcc gcgccacggg cgccgccggc gccgccgcca tcctcgccgc gatcaggcag
     3661 gccggtctcg atcagcggtc gaccgctggt cccggcatcc cgggcgactc cggaacggac
     3721 atcttctcgg tcacggtcga cggtgagacc accgaaactc gactcgccgg cgcggggctc
     3781 ggcgtcgggg gtccgggcgg cccgggcctc ggaggcagcg cggatcccgg caaggccgcg
     3841 gcgttcgacc tgctgaacaa gctcctcgat cccgccgaga cctggggcgg cggtgcgatc
     3901 cagccgtcgt cgtacgcgcc gaccggctat cgcgtgttcg tcatcccggg cgccccgagc
     3961 gtcgatcccg cgacgcccca gacaccggtc gcgtggcccc tctcgaccgc gcttgacgcg
     4021 ttcggcacgg cggccgcgcc ggatcgcggg atcgcgggcc tccgccaagg ggcggtgctc
     4081 gggcctgacg cggtgacgct cgggccgatc ctggccaggg cgacggtcga aaccgccttc
     4141 acgtccggcg gcaagagctt cacgctcgcg gtccgccccc tcctcccgga cgagctgggc
     4201 ggctgacagc gtgccacggg cggttcgggt cggaaggaca gcctgagccg atcggcgcag
     4261 aatccgcccg tgggctgacc cgcagccagg gccaacgggc gggcgccatc ggaaggagcg
     4321 attcgacgga aatggccagg gccaggtgac ctcggaatga gtcggcgcga ccggaaccgc
     4381 ctgcgagggc aggtaccgag gcccgatccg aatgcccctg gtgcggttcc cggccgccat
     4441 gccgtgttcg acgaggacgt cgagccggtc cgccagccgc ggcgccaccg acccgggccc
     4501 gagctcggcg gcgcggtcgg gaccgtccac gccctgctcc cagtccgctt cttcttcggc
     4561 gcgaccttcc tgtacgccgg cctcgacaag ctgatcgacc cgcgcttcct cgacccgtcg
     4621 agtccggcgt cgatccaggc ccagatgatc atcttcatcc gggcatcgcc gatcgcggcg
     4681 ctcgtccgcc tcggccagcc gctggcggtc ccgatcgggc tcgcgatcgc gatcgcggaa
     4741 atcgcgatcg ggctcggcgc cctgaccgga ctcgcctatc gggctgcggc gtggggtggc
     4801 gcgatcctgt cgctcatctt cttcctcacc gcgtcatggg cgacccgccc gtactactac
     4861 gggccggacc tgccgtacgc catcggctgg gtcgccctgg ccagcgccgg ccacggaggc
     4921 ttgttcgtct cgcgtcgcct cctcgcctgg gcgggcggtc gtggttccga gcatgcgctg
     4981 gatcctcgcg gtcggggctc gcgccggccg ctgcgtggcg tgccggccga ggcggacgcg
     5041 acggacagcc ttccactccg cgaccgccgg acggtgttgc aggccggcgt gctcgggttc
     5101 ttcgccctga ttgcggcatc agtggcggtg ccgttccgga tctcccgcgc cggaggggcg
     5161 cccgcggacg gtggaccgga cgcctcggcc ggtgtcgcgg gtggctcgcc cggcgcgtcg
     5221 agcggcccgg gacgatcgag tgcaccacag ccgtcggcgg cgctcggcgg cctcgccgtg
     5281 gcccgtgtcg ccgatctcgc gaagaccgga gcgcgggcgt tcaccgtgcc gttctcggcg
     5341 ccggcaccgt tcccggccgg cgatccggcg gtcatcgtca agctggccga tggatccttc
     5401 gtcgccttcg acgcggtctg cacccacgaa ggctgcacgg tccagtggga tcgggtcgat
     5461 cgggtcctgt tgtgtccatg ccacagcgcc gcgttcgacc cggcccacga cgccgccgtg
     5521 ctccagggtc cgacgaacca gccgctcgca tcgattccga tcgtcgtcga cgcggccagc
     5581 gggacgatct cgctgcgggc gtgatcgcgg cgatccgcgc ggaaggctga cgcgacacgg
     5641 gctgcgtcgc gcagccctcg gtcaggtcgc tggcgggtgg tcggtcgtcg cggcgacctc
     5701 gatcggtggc gccggttcga gcggaccctg gccgtggagc tcgacgagga cggtccggcc
     5761 accgcgcggg ccctgctgat cggcggcaaa gcggacctgg ccggccagcg gttcgccagc
     5821 gcgggtcgag ccgtggagga cgccctcggt cgagccatgg ccggtcttca tcaggacgcg
     5881 ggcggtccat tgctcgtggc cgtcgtcgtc ctcccaggcg tcgatcgtgc aactgccgat
     5941 cggcgtctcg tcgagggtga cctgaagcaa ctcgaagcgg cgatgcctgc gaaccacgac
     6001 tccacggtat caggcccggg cccgaaggcg gtccggccga tatcggcgct cgacgcaagc
     6061 gcctccccgg gtcggtcgag ggggacgcgc atccgcgcac tccctccctc ggcagccggc
     6121 cgcgctgctc gaccgatggg ccgaggtcca accagggctc agcgcggctc gatccggaag
     6181 accgggtgat cggccgggtt cggcagggcc tcgaacggtt cgctcgtccg ttcgcccatc
     6241 tggtcgcgat aggccgtgac gacgcggtcg tgctcgccgc cgctgatctc cttcgcctcg
     6301 accggctcgc ccgtccgacc ccttcgaagg acgccctcgc cggcggcgcg caggttgcgg
     6361 acccagtcgg tctcgccgtc gggggagacg aggtaatgcg ccccgtcgag ctccacggtc
     6421 gcgaccggga ggctgagtgg gtgccccgtc tcctcgtcga tgacggtcag ggtcggcccg
     6481 cccagccaca gcgggtgggt ggggtcgagg cgggtgatcg tcatcgtgga cccggggtgc
     6541 gtcggtgccg gtg
//
LOCUS       DATAIW010000375         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_114216, whole genome shotgun sequence.
ACCESSION   DATAIW010000375 DATAIW010000000
VERSION     DATAIW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114216"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1973)
                     /locus_tag="VHM48_11290"
     CDS             complement(81..1973)
                     /locus_tag="VHM48_11290"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX2756042.1"
                     /translation="MAEGIVYLDVDDEITSAASRIRNAPGTKVALVVPYGSRIATSRM
                     NFRLLSREALVSNRRLSIVSGDAAARSLAASAGLPVFGSVPEYESSLVRPAPESGPSG
                     VGPAGAAAAVAGSLVASETVAAVGRAMGDHAATDPIAEPTPAAMADAPGRKSQRPKRP
                     LPDESATIIQAAPAGLADGIRSPREIEHGPATDDDGEMDLDHSAAAGGRSRAPLVAAI
                     GLIGLAVIVLAVGAYLFLPSASIALTPRRDAIGPIPLTVSADPGATEVDATNNVIPAV
                     RLDVPVEASRTFTTTGSKVKETTATGSVTFTNYDPTSSNTVPSGSIVSTEGGLRFRTL
                     STLIVPRAAFTPPSTTVPSQASVQVQAAQPGTGGNVPANAIRVVPPGESPLFLAVANP
                     NPTSGGTHTETPQVTKAEVDKAVADLQHDLEAAFRQSVAAGANAPPDTTLFPATAALG
                     SSTPDLDPKTLVGQAVDTFDLGLSAHGTVIAVDPRPVTAIAQAQLEAQVTKDHRLIID
                     SVIIEVGAGTVGEDDQVTFQATARATQVAVVDANALRSLVKGKTAAEAQAALVPFGNA
                     TVTLWPDWVATVTSVDVRLSVTVEDGAVNGGGGASPPPSASPRRTGGPASPAPSRAPA
                     SGSSAP"
     gene            complement(1996..>3275)
                     /locus_tag="VHM48_11295"
     CDS             complement(1996..>3275)
                     /locus_tag="VHM48_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEX2756043.1"
                     /translation="RDPLTVAFLRRFWQRDEGESLAACTALDIGTEFAKALVFEIGDD
                     GKGTVRGVGRKRQGLSHMSSGTVADIAAVVDNCSVALQEAEEMAGFRPSQVVIGIAGE
                     LVKGFTTNHSQERKRPDAPITEAELQKLIDTVQREALREAERSITWETGLPNVDVRLV
                     HAAIVGASIDGYALSNPVGFQGRHVKIGIFNAFAPLVHLGALQTVASQLDLELLEIVA
                     EPYAVARVLGSDHIRQAGALFVDVGGGTTDVALVRQGGIEGTRMFALGGRAFTKSLAD
                     RLELPFPRAESAKVDYARGLPVDRREEIGSIIGEDVEVWAAGVELVMEELGAGDLLPG
                     RIYLCGGGSRLPEIEAALRAERFWKRLSFARPPEVTIMAPDQIETIVDATELLVDQQD
                     VTPLGLAYQAIELQTTEAPLDAALRRVLRAMKV"
BASE COUNT          452 a         1158 c         1196 g          469 t
ORIGIN      
        1 caggggcatg gcgatcccct gcgtgtcggc gatcgcgacg ccgatccgct tctcgccgag
       61 gtcgagcccg aggatccggg tcatggcgcg ctcgatccgc tggcgggcgc gcgagacggt
      121 gccggactgg ccggtccacc ggtccgccgc ggcgacgccg acggcggtgg gctggcgccc
      181 ccgccgccgt ttacggcacc gtcctcgacc gtcacgctga gccggacgtc gacgctcgtg
      241 acggtggcga cccagtcggg ccagagcgtc acggtcgcgt tcccgaacgg gacgagggcg
      301 gcctgcgcct cggcggcggt cttgcccttc acgaggctgc gaagggcatt cgcgtcgacg
      361 accgcgacct gggtcgcccg ggccgtggcc tggaaggtga cctggtcgtc ctcgccgacc
      421 gtgcccgcgc cgacctcgat gatgaccgaa tcgatgatca accggtgatc cttggtcacc
      481 tgggcctcga gctgggcctg cgcgatcgcc gtgaccggcc gcgggtcgac cgcgatcacc
      541 gttccgtggg cgctcagccc gaggtcgaac gtgtcaaccg cctggccgac cagggtcttc
      601 gggtcgagat ccggcgtcga cgagccgaga gccgcagtcg ccggaaacag ggtggtgtcc
      661 ggcggggcgt tggcgccggc cgcgacggac tggcggaacg cggcctcgag gtcgtgctgg
      721 agatcggcga cggccttgtc cacctccgcc ttcgtcacct gcggcgtctc cgtgtgcgtg
      781 ccaccgctcg ttggattcgg gttggccacg gccaggaaca gcggactctc gcccggcggc
      841 acgacccgga tcgcgttggc cggcacgttg ccgcccgtcc cgggttgggc cgcctgcacc
      901 tggaccgacg cctgcgacgg gacggtcgtc gacggcggcg tgaacgcggc acgagggacg
      961 atcagcgtcg acagcgttcg aaaacgcagc cctccctcgg tcgagacgat gctgcccgac
     1021 gggacggtgt tgctcgatgt cgggtcgtag ttggtgaacg tcaccgatcc ggtcgcggtc
     1081 gtctccttca ccttgctgcc cgtggtcgtg aacgtccggg acgcctcgac cggcacgtcg
     1141 agccggacgg ccgggatgac gttgttcgtt gcgtcgacct ccgtcgcacc cgggtcggcc
     1201 gagacggtca gcggaatcgg gccgatggca tcgcggcgcg gcgtgagcgc gatcgaggcg
     1261 gagggcagga acaggtacgc cccgacggcc aggacgatga ccgccaatcc gatcaacccg
     1321 atggcggcaa cgaggggggc ccggctgcgg ccgccggccg ctgccgagtg atccaggtcc
     1381 atctcgccgt cgtcgtcggt cgccggaccg tgttcgatct cgcggggaga ccgaatgccg
     1441 tcggcgaggc ccgccggggc ggcctggatg atcgtcgccg actcgtcggg cagcgggcgc
     1501 tttgggcgct gcgacttccg accgggcgcg tcggccatcg cggcgggcgt cggctcggcg
     1561 atcggatccg tcgcggcatg gtcacccatg gcgcggccga ccgcagccac ggtctcgctc
     1621 gccaccagac tgccggcaac cgcggctgca gcgccggccg gccccacccc gctcggcccg
     1681 gactcggggg ccggccggac gagggacgac tcgtattccg ggacggagcc gaacacgggc
     1741 aggccggccg aggccgcgag cgaacgggct gccgcgtccc cggacacgat cgagaggcgc
     1801 cgattgctga ccagggcctc ccgcgagaga agccggaagt tcatccgcga cgtcgcgatc
     1861 cgcgagccgt acgggacgac gagcgcgacc ttggtccccg gggcgttccg gatccgcgac
     1921 gcggccgacg tgatctcgtc gtcgacgtcg agatagacga tgccttctgc catgccggcc
     1981 gaagcctagc ccttcctaga ccttcatcgc gcggagaacc cggcggaggg ccgcatcgag
     2041 cggggcctcg gtcgtctgga gctcgatcgc ctggtaggcg aggccgagcg gcgtgacgtc
     2101 ctgctggtcg accaggagct cggtggcgtc gacgatcgtc tcgatctggt cgggggccat
     2161 gatcgtgacc tccggcgggc gggcgaacga cagccgcttc cagaaccgct cggcgcggag
     2221 ggccgcctcg atctccggca gtcgcgagcc gccgccgcag aggtagatcc ggcccggcag
     2281 caggtcccct gccccgagct cctccatgac cagctcgacc ccggccgccc agacctcgac
     2341 atcctcgccg atgatcgagc cgatctcctc gcgccggtcg acgggcaggc cgcgggcgta
     2401 gtcgaccttg gccgattcgg cccgcgggaa cggcagctcc agccgatcgg cgaggctctt
     2461 ggtgaacgcc cggccgccga gggcgaacat ccgggtcccc tcgatcccgc cctggcggac
     2521 gagggcgacg tcggtcgtcc cgccaccgac atcgacgaac agggccccgg cctggcggat
     2581 gtggtcggag ccgagcaccc gggcgacggc gtacggctcg gccacgatct cgaggagctc
     2641 gaggtcgagc tgcgaggcga ccgtctggag cgcaccgaga tggaccagcg gggcgaacgc
     2701 gttgaagatc ccgatcttga cgtggcggcc ctggaagccg accgggttcg acagggcgta
     2761 accgtcgatc gaggcgccga cgatcgctgc gtggacgagc cggacgtcga cgttcggaag
     2821 cccggtctcc caggtgatcg agcgttcggc ctcgcggagc gcctcccgct ggaccgtatc
     2881 gatcagcttc tggagctcgg cctcggtgat cggggcatcc ggccgcttgc gctcctggct
     2941 gtggttggtc gtgaacccct tcacgagctc gccggcgatc ccgatgacga cctggctcgg
     3001 ccggaagccg gccatctcct cggcctcctg gagcgcgacc gagcagttgt ccaccacggc
     3061 ggcgatatcg gccacggtgc ccgaggacat gtggctcagt ccctggcgct tccgacccac
     3121 gccgcggacg gtgcccttcc cgtcgtcgcc gatctcgaac acgagcgcct tggcgaactc
     3181 ggtcccgatg tcgagggccg tgcaggcggc cagcgactcg ccctcgtcgc gctgccagaa
     3241 ccgacggagg aaggccacgg tcagaggatc ccggg
//
LOCUS       DATAIW010000376         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_173929, whole genome shotgun sequence.
ACCESSION   DATAIW010000376 DATAIW010000000
VERSION     DATAIW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173929"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2152)
                     /locus_tag="VHM48_11300"
     CDS             complement(<1..2152)
                     /locus_tag="VHM48_11300"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX2756044.1"
                     /translation="MPRRLAPRLFIGLVTALLIPAVATAATGDVPIRIPDQAVYDLAG
                     TFDQGTEQSAENLARTVRSIADADIVVVSESVDEGFSAADAATRATTLRLAMGVGDNL
                     DGGGLVVYFGIEPSGCGGQIALNGTADFAAGALPPAVATAIVETDIGPLVTTCDLDSA
                     LLVGMSRIATAVLTADTGTGTAGGGGGTGTGSGQPAVAAGPPFPDNAEGVGVYDHAGL
                     LRPDTIASAEKTIDAIATRTGAQVVVYTQVVEDGRTTEQADQDARALMDQWGVGRKGF
                     DDGLVILFDMYPGLVHGQVILYGGPGFIATFLDNAQKQAIFENDMLPRIKAGDFDGAV
                     LVAIQRVDDAATPEHARALDQARQVNAVIGLVVAPLLAVLLVGSAAWSWLRFGRDPVY
                     LDDPSIHMAGPPEALTPAGAVFVLAGGSSRRALTTALLDLASRGELAFRQESHLLGLK
                     KQVGIETRPADPDPGTRARQLRNAARALGPAERLVRDKLASLGGGADGYIEPDELLKF
                     GASVPDFNKALEEEAVARGWFRETPSKAVARWMVRAAIAGVVGVVAILAGVNVPSSGV
                     LLIGIGLVVGAIIVGVMAQSMPAVSLPGAMIRAMLAAYRRTLKKTMDQARSMDQVVAE
                     AGLPWLETPDQAVVWSTALGLHAEIETVLGRALDDQRDGRVAPGAVWLPTWYGTSAGG
                     AAGFAGDFAGAGSGSGGIFSSSAIPDFGGMMSALG"
     gene            complement(2341..>2632)
                     /locus_tag="VHM48_11305"
     CDS             complement(2341..>2632)
                     /locus_tag="VHM48_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756045.1"
                     /translation="PALEPPRTIDGRPLPVEPPEPPVAVHGPICESTDALGEHDLPPL
                     RRGDLVAIRDAGAYAASLSSTYNGRPRPPQVILTGDGRLVLVRRRGSVAALG"
BASE COUNT          377 a          934 c          973 g          348 t
ORIGIN      
        1 atcccagggc ggacatcatt ccgccgaagt ccgggatcgc cgagctcgag aatatcccgc
       61 cgctgcccga gccggcgccc gcgaagtcgc ccgcgaaccc ggccgcccca ccggccgacg
      121 tgccgtacca ggtcggcagc cacacggcgc cgggcgcgac gcgaccgtcg cgctggtcgt
      181 cgagcgcccg gccgaggacc gtctcgatct ccgcgtgcag cccgagcgcg gtgctccaga
      241 cgaccgcctg gtccggcgtc tcgagccacg gcaggcccgc ctcggcgacg acctggtcca
      301 tcgatcgtgc ctggtccatc gtcttcttca gcgtccggcg ataggcggcg agcatcgccc
      361 ggatcatcgc tccgggcagc gagaccgccg gcatcgactg ggccatcacc ccgacgatga
      421 tcgcgccgac cacgagcccg atgccgatca ggagcacgcc gctgctcggg acgttgacgc
      481 cggcgaggat cgcgacgacc ccaacgaccc cggcgatcgc ggcccggacc atccagcggg
      541 cgaccgcctt cgacggtgtc tcccggaacc agccgcgggc aacggcctcc tcctcgaggg
      601 ccttgttgaa gtccggcacc gacgccccga acttcaggag ctcgtccggc tcgatgtagc
      661 cgtcggcgcc gccgccgaga ctggcgagct tgtcgcggac gagccgctcg gccggcccga
      721 gggcgcgggc cgcattgcgg agctggcgcg cccgggtccc cggatccgga tcggccggcc
      781 gcgtctcgat cccgacctgc ttcttgagcc cgagcaggtg cgattcctgg cggaacgcca
      841 gttcgccccg gctggcgaga tcgagcaggg ctgtcgtgag ggcccggcgc gatgatccgc
      901 cggcaaggac gaagaccgcg ccggcggggg tgagcgcctc gggcggaccg gccatgtgga
      961 tcgacgggtc gtcgagatag acgggatcgc ggccgaagcg tagccacgac caggcggcgc
     1021 tgccgacgag gaggaccgcg agcagcggcg cgacgacgag cccgatgacg gcattgacct
     1081 ggcgcgcctg gtcgagcgcg cgggcgtgct ccggcgtggc ggcgtcgtcg acgcgctgga
     1141 tcgcgacgag gaccgcccca tcgaaatcgc cggccttgat ccgcggcagc atgtcgttct
     1201 cgaagatcgc ctgcttctgg gcgttgtcga ggaacgtcgc gatgaacccc gggccgccgt
     1261 acaggatcac ctggccgtgg acgaggcccg ggtacatgtc gaacaggatg acgaggccgt
     1321 cgtcgaagcc cttgcggccg acaccccact ggtccatcag agcccgggcg tcctggtcgg
     1381 cctgctcggt cgtccggcca tcctccacga cctgggtgta gacgacgacc tgggcgcccg
     1441 tccgggtggc gatcgcgtcg atcgtcttct cggccgaggc gatcgtgtcc ggacggagga
     1501 gaccggcgtg gtcgtagacg ccaacgccct cggcgttgtc ggggaacggt gggccggcgg
     1561 cgaccgcggg ctggccggac cccgtgccgg tgccgccccc gccgccagcc gtcccggtgc
     1621 cggtgtcggc ggtcaggacg gcggtcgcga tcctgctcat cccgacgagg agcgcgctgt
     1681 cgagatcgca ggtcgtgacg agcggcccga tgtcggtctc gacgatggcg gtcgcgaccg
     1741 ccggcggcag ggcgcccgcg gcgaagtcag cggtgccgtt gagggcgatc tggccgccac
     1801 agccagacgg ttcgatgccg aagtacacga cgagcccgcc gccgtcgagg ttgtcgccga
     1861 cgcccatcgc caaccgcagc gtggtggcac gggtcgccgc atcggccgcc gaaaacccct
     1921 cgtcgaccga ctcggagacg accacgatgt cggcgtcggc aatcgagcgg acggtccggg
     1981 cgagattctc ggccgattgc tcggtgccct ggtcgaaggt tcccgccagg tcgtagaccg
     2041 cctggtccgg gatccggatg ggcacgtcgc ccgtggccgc cgtggcgacg gccgggatca
     2101 gcagggccgt gacgaggccg atgaacagcc gtggcgcgag gcgacggggc acgggcagag
     2161 cctagcgcct cgggactggc gcgcccggcc gcgcccgcgt gcgatcaccg tggctgatca
     2221 atttcgccac gaatgagcac ggcccgatca cccgctggac gcggatccgt cggagtggcc
     2281 agtgagtcac gctcattcat cccgaatctg atcagcggcc gtgatcatcg tccggcgcga
     2341 tcagccgagg gcggcgaccg agcctcggcg cctgacgagg acgagccggc cgtccccggt
     2401 gaggatcacc tgcggcggtc ggggccggcc gttgtacgtc gacgacagcg aggcggcgta
     2461 cgcgcccgcg tcgcggatcg caacgagatc gccacgtcgg agtggtggca ggtcgtgctc
     2521 gccgagggcg tcggtcgact cgcagatcgg accgtggacc gcgaccggcg gctcgggcgg
     2581 ctcgaccggg agggggcggc catcgatcgt ccgtggcggc tcgagcgcgg gt
//
LOCUS       DATAIW010000377         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_24490, whole genome shotgun sequence.
ACCESSION   DATAIW010000377 DATAIW010000000
VERSION     DATAIW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24490"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1169)
                     /locus_tag="VHM48_11310"
     CDS             complement(177..1169)
                     /locus_tag="VHM48_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2756046.1"
                     /translation="MDRRRAVGLVLIVISAAGFGSGTVLSKPIYATGLDWLQLLAWRF
                     TIGAALAWAWLLVSPARRAAVRRLGRRPALVAIGLGVWYTGNAATYYAGLETVPASLA
                     GVLVYLYPAIVAILSVRYATRLAGRRPWVALGIALVGVVLALGGIDLRTPPPIGGIAL
                     ILASPLIYAGWIVLSARLAGERSEHLAPESGGAGRVDDAAAASALMITATAVMFVGGA
                     LATGRPYLPSEIPGDAWPYLAAIGFAGTFLAIQTFYAGSRRIGAAQAALVSTVEPLII
                     VTLAWIALHETLAPIQLIGAGLIIVGVLVAQTAERPSGAPEPASPLDAEVPAGG"
     gene            complement(1231..1473)
                     /locus_tag="VHM48_11315"
     CDS             complement(1231..1473)
                     /locus_tag="VHM48_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756047.1"
                     /translation="MDERESDPTGAFRDGGGATGTEHATTIPATGNGEPVTEATGVES
                     EARERAIDAIAVTSDVADPDAQDAVVVDSGEGTPAS"
BASE COUNT          228 a          549 c          575 g          192 t
ORIGIN      
        1 cccgtggtcg acgtcacgtg cgcgtccgga cgtggcccgt cgtcggccgg tcgaaggccc
       61 gaatcgccga cacgcaccat cccggatggt gaatggcagg cggatcggcg cggccggctg
      121 ccgtttcctg agtcgcacca cgccggatgg tgaatggcag aactgacgcg cggcgctcag
      181 cccccggccg gcacttccgc gtccaatggc gaggccggct ccggtgcgcc gctcggacgc
      241 tctgcggtct gggccacgag gacgccgacg atgatcaggc cggcgccgat cagctggatg
      301 ggtgccaggg tctcgtgcag ggcgatccag gcgagcgtca cgatgatcaa cggctcgact
      361 gtgctcacga gggccgcctg ggccgccccg atccggcgcg agcccgcgta gaacgtctgg
      421 atcgcgagga acgtcccggc gaacccgatc gcggccagat acggccacgc gtcgcccggg
      481 atctcgctcg ggaggtacgg ccggcctgtc gccagcgcac ccccgacgaa catgaccgcc
      541 gtcgcggtga tcatcagggc gctcgcagcc gcggcgtcgt cgacccgacc cgcaccgccg
      601 ctctcgggcg cgagatgctc ggaccgttcg cccgccagcc tcgccgacag gacgatccaa
      661 ccggcgtaga tcagcggcga tgcgaggatg agggcgatcc cgccgatcgg tggcggtgtg
      721 cggagatcga tcccgccgag ggcaaggacg acgccgacga gggcgatccc gagcgcgacc
      781 cacgggcgtc tgcccgccag gcgcgtggcg tagcggaccg acaggatggc cacgatcgcc
      841 gggtagaggt agacaagcac gccggcgagc gacgcgggga ccgtctcgag gccggcgtag
      901 taggtcgccg cgttgccggt gtaccagacc ccgagaccga tcgcgacgag ggccggacgg
      961 cggccgagcc tgcggacagc cgcgcgccgt gccggcgaga cgagaagcca cgcccaggcg
     1021 agcgccgctc cgatcgtgaa tcgccaggcg aggagctgga gccagtcgag accggtcgcg
     1081 tagatcggct tcgagagaac cgtgcccgag ccgaacccgg cggccgagat gacgatgagg
     1141 acgaggccca cggcccggcg gcgatccacc gccggaggct agcagccagc gtcgggagac
     1201 gaccgaccgt cgcgcgaccc ggcggtcggg tcagctcgcc ggcgtcccct ccccggagtc
     1261 gacgaccacg gcatcctggg cgtccggatc ggcgacgtcg ctcgttacgg cgatggcgtc
     1321 gatggcgcgc tcgcgggcct cggactcgac cccggtcgct tcggtgaccg gctctccgtt
     1381 gcccgtcgcc gggatcgtcg tcgcatgctc ggtgccggtc gcgccgccgc catcgcggaa
     1441 cgctccggtc gggtcactct cgcgctcgtc catggtcgat ctccttgtcg cgggctggcg
     1501 ggggagcatg cacccggggc agttgcgccg gatcgggcga cggc
//
LOCUS       DATAIW010000378         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_249176, whole genome shotgun sequence.
ACCESSION   DATAIW010000378 DATAIW010000000
VERSION     DATAIW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249176"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VHM48_11320"
     CDS             complement(<1..292)
                     /locus_tag="VHM48_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756048.1"
                     /translation="MIDDAGPQQDGEDASPSLTMPEKVVAEIPPTAADDKALSAADLA
                     PTADLASESVAHPADIDAAPALAPTADLAPTVNLTTPADLSQPADLSQPADLS"
     gene            complement(289..1011)
                     /locus_tag="VHM48_11325"
     CDS             complement(289..1011)
                     /locus_tag="VHM48_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756049.1"
                     /translation="MNRTDSLRHIGARLAPGRVLLASARLWQSVRPTTRRGAALALAG
                     IVAGSGLLAVVFLVGAAMAWSPYVESVIHPDGNARTWAGLDRTFAGAGLCSQCHQVES
                     ARAASARHAGIGCESCHGALLGHALPGQDQTSSTTGPALPTDSVCIRCHTTVIGRPAD
                     LLQINLAGHYVPECLQCHDPHTGISRRPPVVLHPLVDLPACVTCHGPDGFKARSQRHP
                     VVSGDEPCLLCHAQGRGPESRS"
     gene            complement(1014..2648)
                     /locus_tag="VHM48_11330"
     CDS             complement(1014..2648)
                     /locus_tag="VHM48_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX2756050.1"
                     /translation="MPRSRGGIVALLLVLGGIGAGLTFGASAAIGWTETADFCGRCHQ
                     MGPELAAYEAGPHRDVACAECHVEPGVGGWVKAKLNGTKQLIQVVTGLYPKPVPPPDH
                     SNLPPVSVSCIRCHSKDRLAASAVVTTTGYAEDEGNTREFIGLMIRPGGGDTFDVNRS
                     VHWHVLQDVEFVAADQNSQKIDYVRVARPNGTVEEFLSQDQIRDSADVKPDIKRVIAE
                     RAPRRMDCLECHNRAGHPIPNPRKGIDQALSTGRLDITLPFLKREGMSLLVADYPTVE
                     AADAAIASLRGFYTLHYPDVANRQAPEIAAAVTELQRLYRLVAMPDMKVSAKTYPDNL
                     GHTDFPGCFRCHDGGHYLVRNGAATKEVIPATCDTCHTFPQLGPGVASIPFGKPPDTH
                     DDRLWVFDHRNVATSIDPGGQSCGQCHAKDYCENCHKTGAITVKHDEMLLNHAAVIRI
                     SGNAACAYCHQRPYCSRCHSEDVLPANGLGGIDPGGTGAIDVDVGPTGSLPTRARTRR
                     SATGLLPVRAPTRRASSWRARTRRARIRRSARRPEG"
     gene            complement(2808..3398)
                     /locus_tag="VHM48_11335"
     CDS             complement(2808..3398)
                     /locus_tag="VHM48_11335"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2756051.1"
                     /translation="MNDSIGPRPGRLLLGARTRTALVVAVTAVAIGVTAFLIDRPAAA
                     GGFTPSGITSGAPPQAGEKVPDFEARTPDGKTVKLSDFAGRPLWLSFGATWCPDCRAE
                     AADVEAAYLANQAKNLAILTVWVNDDSTNVTTFAAKNGFTFAMVLDPDQRIADGYRLL
                     GYPTHFFVDPEGIIQVVRLGRLTPDDMARSITEIAN"
     gene            complement(3424..4314)
                     /gene="ccsB"
                     /locus_tag="VHM48_11340"
     CDS             complement(3424..4314)
                     /gene="ccsB"
                     /locus_tag="VHM48_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX2756052.1"
                     /translation="MFKMSEYTFVAAALCLGLASLIYLAYAVSGLRAGMLRTAGGPGG
                     ASSTVTFASGPYTSELGRYGTIIGWLAMVTLTVSLIFRTIATGHGPFANMYEFSIAFA
                     WGILVAYYWFERRYSQRILAVVAMPVALGLMLYAVTIPSTIEPLVPALQNNLLLTIHV
                     AVAIVAYGSFTVGFGAALLYLVQPEQGRWGLPKPKILEEIGYRAIVVGFPFLTLTVIL
                     GAVWAEVAWGKYWSWDPKETASLLTWLIYATYLHARVVRGWRAKRAAWLLMLGFAATL
                     FTYFGNLYFGGLHSYAGLAQ"
     gene            complement(4335..5807)
                     /locus_tag="VHM48_11345"
     CDS             complement(4335..5807)
                     /locus_tag="VHM48_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2756053.1"
                     /translation="MAQTLQTSSAPGDARITGAIPAGAASAGLLDGILDGLWHGLTSM
                     RFAIALTLIAAALSFVGALVMQAPAGVLADANAKAAWIAQVRPKYGAFTDVFDALQVF
                     NIFNSLWFRLVGGLLVASITACTAHRTPAVWRTATKPTVDVGEAFFEHAPQHEAMVVH
                     RSADEALAAVRTVLGKRRFRTLVHNDGTMHLYADRNRWGPFGSLVGHLAFVVILAGAM
                     IGGALGFQNPDFTIAEGSTLTVPSATGLSIRLDAFRDSYDPATGMPLDYASQVVLFED
                     GTEVARHTIRVNDPLRYDGISFFQSFFGPAVILSATADGGSPLFSGGVPLAWSADDSD
                     RAIGTLDLPGTGKVAWVAGTSGGNDTVVRPGQLLVEIYQADGAGTLVERKTIDQGVAT
                     TVGGVTFTFQREEKFTGLAISRDPGAPLIWLGSLLLFGGFVLVLMFPHRRLWGRIAPR
                     PDGGATLLVGSIGRKDTTAGIEFTELVNDLRAVLHAPART"
     gene            complement(5819..6571)
                     /locus_tag="VHM48_11350"
     CDS             complement(5819..6571)
                     /locus_tag="VHM48_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2756054.1"
                     /translation="MTGLTIVGLLIAFGGGILSFASPCCLPLIPAYVSYMVGTTGGSV
                     RPDGRPDRRPALAHGLSFVLGFSLVFVAFWASIGLVGYVLAGNARILHQLGGGLLVFL
                     GLQTAGVINVRLLWSDTRAMPVMGGLGSGGVRLAAQPSYGRSAAFGALFAAGWSPCIG
                     PILGGIIGLASASASVGEGTALLIAYAAGLAVPFLAVALGADAVAQRLGWVGRHHQAV
                     SRLTGLLLVAIGLLMITNLLGRLAQVTSPFGL"
     gene            complement(6568..>6883)
                     /locus_tag="VHM48_11355"
     CDS             complement(6568..>6883)
                     /locus_tag="VHM48_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2756055.1"
                     /translation="LGLGAAAFNGGDSTAAEAAWKKVVAIDPKSVEAHYDLGLLYLNL
                     NDLVSVKREWELVVQLDPGSDVAKNVQDHLDAIAAQASPGVGSSSSPAAPPAVPSGSA
                     RP"
BASE COUNT         1071 a         2253 c         2553 g         1006 t
ORIGIN      
        1 gcgacaggtc agccggttgc gacaggtcag ccggttgcga caggtcagcg ggggtcgtca
       61 ggttgacggt gggtgccagg tcggcggtgg gtgccagggc gggcgccgcg tcgatgtccg
      121 caggatgcgc caccgactca cttgcgaggt cggcggtcgg cgccagatcg gccgcactca
      181 gggctttgtc atcggcggcg gtcggcggga tctcggcgac gaccttctcg ggcatcgtga
      241 ggctgggcga cgcgtcttcg ccgtcttgct gcgggccggc gtcgtcgatc acgatcggga
      301 ctccgggcca cgcccctgag cgtgacacaa gaggcagggt tcgtcgcccg acaccacggg
      361 atgacgttgc gatcgggcct tgaatccatc cggcccgtga caggtgacgc aggccggcag
      421 gtcaacgagt ggatggagga cgaccggcgg tcgccgcgag atcccggtgt gcgggtcatg
      481 gcactgcagg cactccggga cgtaatggcc ggcgaggttg atctgaagaa ggtcggcagg
      541 acgcccgatc acggtcgtgt ggcagcggat gcagacgctg tcggtcggca gcgccggccc
      601 cgttgtcgat gaggtctggt cctggccggg cagcgcgtgc ccgagcaggg ctccatgaca
      661 gctttcgcag ccgatgccgg cgtgccgcgc ggacgccgcc ctggcgctct cgacctgatg
      721 acattgcgag cagagtccgg cgcccgcgaa ggtccgatcg aggcccgccc acgtccgggc
      781 gttgccgtcg gggtggatca cggactcgac gtatggcgac caggccatcg cagcaccgac
      841 gaggaaaacg acggccagca gtccggaccc ggcgacgatc ccggcgagag cgagggccgc
      901 tccccggcgg gtcgtcggtc ggacgctctg ccacagacgc gccgacgcga gcaggacacg
      961 gcccggggcg agccgtgcgc cgatgtgtcg aagcgagtcc gtgcggttca cggtcagccc
     1021 tcgggccttc gagcggatcg ccggatcctg gctcgcctgg tcctggctcg ccagctgctg
     1081 gctcgcctcg tcggcgctcg cacgggcaac agcccggtcg cggatcgcct ggtccgagct
     1141 cgggtcggca gcgaaccggt cggaccgacg tcgacgtcga tcgcccccgt gccgccggga
     1201 tcgatgccgc ccagaccgtt cgccggcagg acatcctcgc tgtggcagcg agagcagtac
     1261 gggcgctgat ggcaataggc gcaagccgcg ttgccgctga tccggatcac cgccgcatgg
     1321 ttcaggagca tttcgtcgtg cttcacggtg atggcgccgg tcttgtggca gttctcgcag
     1381 tagtccttgg cgtggcattg gccgcacgat tggccgccgg gatcgatgct cgttgcgacg
     1441 ttccggtgat cgaagaccca caaccggtcg tcatgagtgt cgggcggctt gccgaacggg
     1501 atgctcgcga cgccagggcc gagctgcggg aaggtgtggc acgtgtcgca ggtcgccggg
     1561 atgacctcct tcgtcgccgc cccgttcctg accaggtagt ggccgccgtc gtggcagcgg
     1621 aagcaacccg ggaaatcggt atggcccagg ttgtccggat aggtcttggc gctgaccttc
     1681 atgtccggca tcgcgacgag gcgatagagc cgctggagct cggtgaccgc cgcggcgatc
     1741 tccggcgcct ggcgattggc gacatcgggg taatgcaagg tgtagaagcc gcgcaatgac
     1801 gcgatcgcgg cgtcggcggc ttcgaccgtc ggatagtccg cgacgagcag gctcatcccc
     1861 tctcgcttca ggaacgggag ggtgatgtcg agccggccgg tgctgagcgc ctggtcgatc
     1921 cccttgcgcg ggttcgggat cggatgaccg gcccggttgt ggcactcgag gcagtccatc
     1981 cggcgcggcg ctcgctcggc gatgacccgc ttgatgtcgg gcttgacgtc ggcgctgtcg
     2041 cggatctggt cctgggacag gaattcctcg accgtgccgt tggggcgggc gacccggaca
     2101 tagtcgatct tctggctgtt ctggtccgcg gccacgaact cgacgtcctg gaggacgtgc
     2161 cagtgaacgc tgcgattgac gtcgaacgtg tcgccgccgc cgggccggat catcagcccg
     2221 atgaactcgc gggtattgcc ctcgtcttcc gcgtagccgg tcgtcgtcac gaccgccgat
     2281 gcggccaggc ggtccttgga gtggcagcga atgcacgaca cggagaccgg cggcaggttc
     2341 gagtggtcgg gcggcggcac gggcttcggg tagaggccgg tgaccacctg gatcagctgc
     2401 ttggtgccgt tgagcttggc cttgacccag ccgccgacgc ccggttcgac gtggcactcg
     2461 gcgcaggcga catcgcgatg cggtccggcc tcgtaggcgg cgagctcggg gcccatctgg
     2521 tggcaccgtc cgcagaagtc ggcggtctcg gtccagccga tggccgcgga ggcaccgaag
     2581 gtcagtccgg cgccgatccc gccaaggacg aggaggaggg cgacgatccc gcctcggctt
     2641 cgtggcatcc gcaggcgccg gaaccgacgg cgcccgtgcg gggccgcggt ggggggctcg
     2701 gcggatggcc ccggagcgac gtcagtcgcc ggaagcgcgg gcgccgcggc cgtggtggtc
     2761 ggatcccctc gcgacgcgtc cggcgacagc tcgggggtcg gcatcggtca gttcgcgatc
     2821 tcggtgatcg acctggccat gtcgtcgggt gtgagccggc ccaggcgcac gacctggatg
     2881 atcccttccg ggtcgacgaa gaagtgggtg gggtagccga gcaggcggta gccgtcggcg
     2941 atccgctggt ccgggtcgag gaccatcgcg aacgtgaagc cgttcttggc ggcgaacgtc
     3001 gtgacgttcg tggagtcgtc attgacccag accgtcagga tcgcgaggtt ctttgcctga
     3061 ttggcgaggt acgcggcctc cacgtcggcg gcttctgccc gacagtccgg gcaccatgtc
     3121 gccccgaagg acaaccagag cggccgaccg gcgaagtccg acagcttgac cgtcttgccg
     3181 tcgggcgtcc gggcctcgaa gtccgggacc ttctcgccgg cctgcggtgg agcaccgctc
     3241 gtgatgcccg acggtgtgaa cccgcccgcg gccgcgggcc gatcgatcag gaacgcggtg
     3301 accccgatcg cgaccgcggt caccgccacg acgagcgcgg tccgagtccg ggcgccaagg
     3361 agaaggcgcc ccggcctggg tccgatgctg tcgttcatgt cacgcctccg acgtggcccg
     3421 accctactgg gcgagcccgg cgtagctgtg cagcccgccg aagtagaggt tgccgaagta
     3481 ggtgaagagc gtggccgcga agccgagcat caggagccac gcggctcgct tggcccgcca
     3541 gccgcggacg acgcgggcgt ggagataggt cgcgtagatg agccacgtca ggagcgacgc
     3601 ggtctccttc ggatcccagc tccagtactt gccccacgcc acctcggccc agacggcccc
     3661 gaggatgacg gtcagggtca ggaacgggaa gccgacgacg atcgcgcggt agccgatctc
     3721 ctcgaggatc ttgggcttcg gcaggcccca gcggccctgc tcgggctgga cgaggtagag
     3781 aagcgccgct ccgaagccga ccgtgaagga gccgtaggcg acgatcgcga ccgccacgtg
     3841 gatcgtgagg aggaggttgt tctggagggc cgggaccagc ggttcgatgg tcgaggggat
     3901 cgtgaccgcg tagagcatga gcccgagggc gaccggcatg gccacgaccg cgaggatccg
     3961 ctggctgtag cgccgctcga accagtagta ggcgacgagg atgccccacg cgaacgcgat
     4021 ggagaactcg tacatgttgg cgaacgggcc gtggccggtg gcgatggtcc ggaagatcag
     4081 cgagaccgtc agcgttacca tcgcgagcca gccgatgatc gtgccgtagc ggccgagctc
     4141 cgaggtatac ggtccgctcg cgaaggtcac cgtgctcgat gccccgcctg ggccaccggc
     4201 cgtccggagc attccggctc gcaggccgga gacggcgtac gcgaggtaga tcaggctcgc
     4261 caggccgagg cacagggccg cggcgacgaa cgtgtactcc gacatcttga acatgacgtg
     4321 gattcccttc cttgctaggt ccgcgcgggc gcgtgcagca ccgcgcgcag gtcgttgacg
     4381 agttcggtga attcgatgcc ggccgtggtg tcttttcggc cgatcgagcc cacgaggagc
     4441 gtggcgccac cgtcgggtcg cggggcgatc cggccccaca gccggcggtg cgggaacatc
     4501 aagacgagga cgaagccgcc gaagagaagg agcgatccga gccagatcag cggtgcgccg
     4561 ggatcgcgcg agatcgccag gccggtgaac ttctcctcgc gctggaacgt gaacgtgacg
     4621 ccgccgaccg tcgtggccac gccctggtcg atggtcttgc gctcgaccag cgtgccggcg
     4681 ccgtcggcct ggtagatctc cacgaggagc tgtcccggcc gcacgacggt gtcattgcca
     4741 ccgctcgtgc cggccaccca ggcgaccttg ccggtgcccg gcagatccag cgtgccgatg
     4801 gcgcgatcgc tgtcgtcggc gctccaggcc aggggaactc caccgctgaa gagcggcgag
     4861 ccgccgtccg cggtggcgct caggatcacc gccggtccga agaacgactg gaagaagctg
     4921 atcccgtcgt agcgcagcgg gtcgttgacc cggatcgtgt ggcgggcgac ctcggtccca
     4981 tcctcgaaca ggacgacctg gctcgcgtaa tcgaggggca tccccgtcgc ggggtcgtag
     5041 ctgtcgcgga aggcgtccag acggatcgac aggccggtcg ccgacggcac cgtcagggtg
     5101 ctgccttcgg cgatcgtgaa gtccggattc tggaagccaa gggcgccgcc gatcatcgct
     5161 ccggccagga tcacgacgaa cgcgaggtgg ccgacgagcg atccgaacgg gccccagcgg
     5221 ttccgatccg cgtagagatg catcgtcccg tcgttgtgga ccagggtccg gaagcgccgc
     5281 ttgccaagga ccgtccggac ggccgccagg gcctcgtcag cggatcgatg gacgaccatc
     5341 gcctcgtgct gtggcgcatg ctcgaagaag gcctcgccga cgtcgaccgt cggcttggtc
     5401 gcggtccgcc agaccgccgg cgtgcggtgc gccgtgcagg ccgtgatgct cgcgaccagg
     5461 aggccgccga cgagccggaa ccacagcgag ttgaagatgt tgaacacctg gagggcgtcg
     5521 aacacgtcgg tgaaggcgcc gtacttcggg cgcacctggg cgatccaggc ggctttggcg
     5581 ttggcgtcgg cgagcacgcc cgccggggcc tgcatgacaa gcgctccgac gaacgacagg
     5641 gccgccgcga tgagggtcag ggcgatggcg aaccgcatcg atgtcaggcc gtgccagagg
     5701 ccatcgagga tcccgtccag cagcccggcc gaggccgcgc cggcggggat cgcaccggtg
     5761 atccgggcgt cccccggggc tgagctggtc tgcagcgtct gagccatggt tcccttcctc
     5821 acaagccgaa cggcgaggtg acctgcgcca gccggccgag caggttggtg atcatcagga
     5881 gtccgatggc gaccagcagg aggccggtca accgggaaac cgcctggtgg tggcggccga
     5941 cccagccgag tcgctgcgcc accgcgtcgg cgccgagtgc cacggcgagg aacgggacgg
     6001 ccaggcctgc ggcgtaggcg atcagtaggg cggtcccctc accgacactg gcgctcgccg
     6061 aggcgagccc gatgatcccg ccgaggatcg gcccgatgca cgggctccac ccggcggcga
     6121 acaacgcgcc gaaggcggcc gaccggccgt aggacggctg cgcggcgagt cggacacccc
     6181 cgctgccgag gccgcccatc accggcatcg cccgcgtgtc gctccagaga agccggacgt
     6241 tgatgacgcc cgcggtctgg aggccgagga agaccaggag gcctccgccg agctggtgaa
     6301 ggatccgggc attcccggcc aggacgtacc cgacgaggcc gatcgacgcc cagaaggcga
     6361 cgaagacgag cgagaagccg agcacgaacg acagcccgtg agcgagcgcc ggccggcggt
     6421 cgggccggcc gtccgggcgg acgctcccgc ccgtcgtccc gaccatgtag ctgacatagg
     6481 ccgggatcag cggcaggcag cacggcgagg cgaacgacag gatcccgccg ccgaaggcga
     6541 tcaggaggcc gacgatcgtc aggccggtca tggcctggcg ctcccgctcg ggaccgccgg
     6601 cggagcggcg ggggagctgc tcgagccgac gccgggcgag gcctgggccg cgatcgcgtc
     6661 gaggtgatcc tggacgttct tcgcgacgtc cgagcccgga tcgagctgga cgacgagctc
     6721 ccactcgcgc ttgacgctga cgagatcgtt gaggttgagg tacagtaacc cgaggtcgta
     6781 gtgcgcctcg acggacttcg ggtcgatggc gacgaccttc ttccaggccg cctcggcagc
     6841 ggtcgagtcc ccgccgttga aggccgccgc gccgagtccg agg
//
LOCUS       DATAIW010000379         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_114536, whole genome shotgun sequence.
ACCESSION   DATAIW010000379 DATAIW010000000
VERSION     DATAIW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114536"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1568)
                     /locus_tag="VHM48_11360"
     CDS             complement(<1..>1568)
                     /locus_tag="VHM48_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756056.1"
                     /translation="TGISDVGSWYLVVNADRESALTNERVDLSGGEHIVIRAWPEDAA
                     WQKRVSALLTTGLPELVEQTGLNWPVTGDLSIFEVHTPLLEGYAGVFLQGEDKIEISE
                     DLDDLTILHEASHAWFNGDLFDGRWINEGFADTYAAKALDGIGSPSVTPGDVSPTDKA
                     AVRLVDWVHPGRITDAETDAREQYGYDASWTVIRSIVREVGDPGMQHVLTAAEDHQIA
                     YVGSGEPEVVGGSNDWRRLLDLLDELGRSAKADDVFRRWVVTDAQAGMLDERASARTA
                     YAALARAGGDWQAPYYVRGRMSDWDFPAATTRIAEAIALLARRDQIAAIAVPLGVTPP
                     ADLRTAYQGARDSLDDANHVADSETAAVRALATATVAVAAPRAPLVALGLLGTTPEIV
                     LAAARSAFSAGDADAGARAAAVTALIDGAVEVGRGRLIAAIAGVVVAVILLVLALVLL
                     RRRRRRQRASALAGPGADAGAAGGEPPYATLADQSNRALDADPTAARPTSVDPMDAPD
                     PPDSPDPPDPPGPP"
BASE COUNT          208 a          562 c          571 g          227 t
ORIGIN      
        1 tcgggcggac cgggcgggtc tggcgggtct ggcgaatcgg gcgggtctgg cgcgtccatc
       61 gggtcgacgg acgtcggtcg tgccgcggtc gggtccgcgt cgagcgcacg attcgactgg
      121 tcggcgagtg tagcgtacgg cggctcgccg ccagcggcgc ccgcatccgc gcccggtccc
      181 gccagtgccg acgcccgctg ccgtcgccga cggcggcgaa gaagaacgag ggcgaggacg
      241 aggaggatca ccgccaccac cacgcccgcg atcgccgcga tcaaccggcc gcgcccgact
      301 tcgaccgcgc catcgatcag ggccgtcacg gccgccgccc gtgccccggc gtcggcgtcg
      361 cccgcactga acgccgaccg ggcggcggcg aggacgatct cgggcgtcgt cccgagcagg
      421 ccgagcgcga cgagcggggc ccgcggcgcc gcgaccgcca cagtcgccgt ggccagggct
      481 cggaccgcgg ccgtttcgct gtcggcgacg tggttcgcgt cgtccaatga gtcgcgagcc
      541 ccctggtagg cggttcgcag gtcggccggc ggcgtgacac cgagcggcac ggcgatcgcc
      601 gcgatctggt cacgacgcgc gagcagcgcg atcgcctcgg cgatccgcgt cgtggcggcg
      661 gggaaatccc agtcgctcat ccggccgcgg acgtagtacg gcgcctgcca gtcgccgcca
      721 gcgcgggcga gcgccgcgta cgcggtccgg gcagaggccc gttcgtcgag catcccggcc
      781 tgggcatcgg tcacgaccca gcgccggaag acgtcatcgg ccttcgccga acgaccgagc
      841 tcgtcgagca ggtcgagcag tcgccgccag tcgttcgacc cgccgacaac ctccggctcg
      901 ccgctcccga cataggcgat ctggtggtcc tcggcggccg tgaggacgtg ctgcatcccg
      961 gggtcgccga cctcccggac gatggatcgg atgaccgtcc acgatgcgtc gtagccgtat
     1021 tgctcgcggg cgtccgtctc cgcgtcggtg atccggcccg ggtgaaccca gtcgacgagc
     1081 cgaacggcgg ccttgtcggt cgggctgacg tcgccgggcg tgacactcgg gctcccgatc
     1141 ccatcgagcg ccttcgccgc gtacgtatcg gcgaagccct cgttgatcca gcgcccgtcg
     1201 aacaggtcgc cgttgaacca ggcgtgggag gcttcgtgga ggatcgtgag atcgtcgagg
     1261 tcctcgctga tctcgatctt gtcctcgccc tggaggaaca cgccggcata gccctcgagc
     1321 agcggggtgt ggacctcgaa gatcgacagg tcgccggtga ccggccagtt cagcccggtc
     1381 tgctcgacga gctcgggcag cccggtggtg agcaacgcgc tgacgcgctt ctgccaggcg
     1441 gcgtcttctg gccacgcccg gatgacgatg tgctcgccgc cgctcaggtc gacccgttcg
     1501 ttggtgaggg cgctctcgcg gtccgcgttg acgacgaggt accagctccc gacatcgctg
     1561 atcccggt
//
LOCUS       DATAIW010000380         8256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_85104, whole genome shotgun sequence.
ACCESSION   DATAIW010000380 DATAIW010000000
VERSION     DATAIW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85104"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3018)
                     /locus_tag="VHM48_11365"
     CDS             complement(<1..3018)
                     /locus_tag="VHM48_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2756057.1"
                     /translation="MPREPGDERSRRGTGQRAERRSASDPFARLGRFAVRRRWAIVLV
                     WAVILLVAIPLAPRVVGVLRAGGFILDDLESARAKALLQAELASPPSAVVVVYHSAVG
                     TAGTPPFEAAAAAAIQHVPEAPYVVRILPHTLSPRQVSTDGHTAYDIVFMSIAPDDSP
                     AALPGIRERLAQPAGLEVQLAGGPAFYGDVQSVSESDLRRSEVVSLPLAALALLLVFG
                     SVAAAALPLIVGGAAVVVALAAIFLVGSVTPMSIFVLNLATLLGLGLGVDYSLLMTSR
                     FREEMAARAADPPAERVAGAVEATVETAGRAVFFSGLTVLLGLIGLVLFEFMILRSVG
                     IAGAIVVLLAASSALTLLPALLAIVGPRIDALAIRRVIPRDDPNGPWARLARRVMRHP
                     VAVLVPTLGVLLVLGSPFLHVRFNAPDSTILPASVPSRAAFDRLANAFGEGEFAPIVL
                     AIRTAGAATSTDNVAALYDYSRRLAADPRIRRIDSFVDVDPRLRKDQYQLLYADPNGP
                     RDRFVATALAATTNGNLTAFTITTPFGPNRDEGRSLVTALRDPAGPLAPPAGMTVLVG
                     GGAADVSDVVGRVAQDFPRTGLFIVVTTYLVLFMLLRSVVLPAKALVMNTLSIVASFG
                     ALVWIFQDGNLSALLGFQPLGFVETTQPVILFCVLFGLSMDYEVFLLTRMKEQWDRTG
                     DNQEAVARGLERSGRIVTSAALIVVVVAGSFAFADIVLIKALGLGMALAVALDATVVR
                     ALLVPATMRLLGHWNWWLPAALRRVPKIETAGIVGLIALALIAGPLLAGCTTAGAPIL
                     ANPAAPHPAPPALTPAPSAVADPQAIALPRDDAPHDRLTEWWYYTGHLHTADGRRFGF
                     EDVIFRAERGGFPVTWASHLAITDEIGNAFHYAQRTEIGPQVDRLGSGGTFSLAVADL
                     AGGTPWTMSGSGGTDILHADFSPGEAAAAGSPGGLGLGLGLTSAKPAALHNTNGWIDF
                     GAGGSSYYYSRTSMVANGSLTIDGSRLEVTGTAW"
     gene            3128..3640
                     /locus_tag="VHM48_11370"
     CDS             3128..3640
                     /locus_tag="VHM48_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2756058.1"
                     /translation="MTIDASERDPRARAGSTDATGDWAAISAWARDLAARIGSEQPTQ
                     HIAIFVPDDDGGRLRLAAQVWGAGEDTGEVIVGQWLVPLEGSVCGRVFRTGLAALCAD
                     VGMDPDYRAFPGGRTRSSLTVPVGPPDGVAAVINLEAPWVGAFSIRDYERMTEEAAAA
                     YASFPVRRVA"
     gene            3763..4083
                     /locus_tag="VHM48_11375"
     CDS             3763..4083
                     /locus_tag="VHM48_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756059.1"
                     /translation="MQLFTIFFSRPVEFGVATAITVLMLVILLRAAISSEGPSGLGRF
                     MARPTSKFVFGFLFLAWAVVFGIGLQLVPHEGANSPYGGLGLVAMFSGFFIMMGFIWS
                     VIGE"
     gene            4186..5928
                     /locus_tag="VHM48_11380"
     CDS             4186..5928
                     /locus_tag="VHM48_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756060.1"
                     /translation="MTTSRLRSTSHRPTARRGTIRAAATAIASAAILLAGVLPTSVAA
                     AGDLTPPTGSVEYWTIDADTQLAEFRFHYTDPESGLDHITVVCDGSAPITIPYSATVL
                     WAIHDGTGGCTTAYGDHDVWIEVFNGDGLGAPGTGFTVTNGPSLHLTVSTAVTGHPIT
                     ITPVFPSDFTVPAGDGCRWEFRWGTNRALDQGFADETFGGLLFDVRASDGGCGPWTFT
                     LPWVPYRQFDVRVDVFHQDPDGGFVEGPGAQHRFTAAADGTDRRVLTSNLPIAQVLPS
                     TYTPIVGTPVTYTRYLVGGATVCCNANWVARLGNGENPIVWTQNGGSTFTFTPSATGN
                     LLVSWAREGSTGLVLSGYYDPPVRYRDTTAPVATAPRERIRPMAASETVPIAIEWGGT
                     DRGWGIASFQLQRSLNGGGWISVTLPSVTATSVGINGVVGTTLRFRVRAKDKAGNVGS
                     WVYGSTFRVSRVSDASTTIRYSTGWTATARPGAYGGMLHATAGAGAAMSYTFFGRDVA
                     WIASRGPDYGRAKIYLDGAYIGSIDLFATSPEARRIVFARHWTSVGTHTLRIVNVATS
                     GRPWIDVDGFAVLR"
     gene            6043..6294
                     /locus_tag="VHM48_11385"
     CDS             6043..6294
                     /locus_tag="VHM48_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756061.1"
                     /translation="MTAVLASRDLRNGNEGVLRFDPRTGTYQVGMLESGSLPVSRAHL
                     VLVNARTGDIVGFVERDWDYAVDGFPSARAGTSGPLDRP"
     gene            6392..8056
                     /gene="ablA"
                     /locus_tag="VHM48_11390"
     CDS             6392..8056
                     /gene="ablA"
                     /locus_tag="VHM48_11390"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX2756062.1"
                     /translation="MDADEIKTAVARQAAERAAAELPFVNRRDPAQMLGPDGRPAVSR
                     RAPIWEDVPDEKWNDWRWQLSHRVNDLEEIEQILNLTEDEREGLSAKDKFRVDITPYF
                     ISLIDPDDPNDPIRRQVIPLGREQQAFTAMMEDSLAEDRHSPVPGLVHRYPDRVLMLV
                     TTQCASYCRYCTRSRIVGDPTQNFNRKDHEAQLDYLRRTPQVRDVLISGGDGLTLAPK
                     LFESILRGLREIPHIEIIRIGSRVPVFLPQRIDDELCAMLEKYHPLWINLHFNHPNEI
                     TPEVSRAVDKLTKAGLPVGNQSVLLAGVNDCVHIQRSLVHKLVENRIRPYYLYQCDLV
                     EGSGHFRTPVGKGLEIMEGLRGHTSGYAVPTYVIDAPGGGGKIPVMPNYLISYSDHKV
                     VLRNYEGYITTYEEPPTYERHDEAACSYCQSQRSEPGQSGVLGLLQGERMWIEPAGFE
                     LTHSRGNTQAHRLQDPAKWVPFGVGAIEGQATRPLRVLEAGEHLLDDFDRAATETNAG
                     IVEPGDLESEEERAAHARDAAPAYSPGEEPRPRDGEPTGSAHGELL"
     gene            complement(8053..>8256)
                     /locus_tag="VHM48_11395"
     CDS             complement(8053..>8256)
                     /locus_tag="VHM48_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2756063.1"
                     /translation="IEDRIDHDLDAGSTAGLIVEIEELVREEPFRERLWRQLMLAQYR
                     SGRQAEALATFQRARREPGAGDA"
BASE COUNT         1283 a         2925 c         2897 g         1151 t
ORIGIN      
        1 ccaggccgtg ccggtcacct cgagtctgga tccgtcgatc gtgagggagc cattcgcgac
       61 catcgacgtc cgcgagtagt agtacgacga gccgccggca ccgaagtcga tccagccgtt
      121 cgtgttgtgg agtgccgccg gcttcgccga ggtcagcccg agcccgagcc cgagcccgcc
      181 cgggctgcct gccgcagcgg cttccccggg cgagaagtcc gcgtgcagga tgtccgtgcc
      241 gccgctgccg gacatcgtcc acggcgtccc gcccgccagg tccgcgacgg caagactgaa
      301 ggtgccaccg ctcccgagcc ggtcgacctg cggcccgatc tccgtccgtt gggcgtagtg
      361 aaaggcgttg ccgatctcat cggtgatcgc gaggtgcgag gcccacgtca cggggaaccc
      421 gcctcgctcc gcccggaaga tcacgtcctc gaacccgaag cggcggccat ccgccgtgtg
      481 gagatggccc gtgtagtacc accactcggt caggcggtcg tgcggcgcat cgtcgcgggg
      541 cagggcgatc gcctgcgggt cggcgaccgc cgagggcgcc ggcgtgaggg cgggcggcgc
      601 cggatgcggt gcggccggat tggcgaggat cggcgctccg gcggttgtgc accccgcgag
      661 cagggggccg gcgatgaggg cgagggcgat caggcctacg atccccgccg tctcgatctt
      721 cggcacccgc cggagggcgg ccggcagcca ccagttccag tgcccgagga gccgcatcgt
      781 ggccggcacg aggagcgccc ggacgacggt cgcgtcgagg gccaccgcga gcgccatgcc
      841 gaggccgagc gccttgatca ggacgatgtc ggcgaaggcg aaggagccgg cgaccacaac
      901 gacgatcagc gccgccgagg tgacgatccg gccgcttcgc tcgaggcccc gagccacggc
      961 ctcctggttg tcgccggtcc ggtcccactg ctccttcatc cgcgtcagga ggaacacctc
     1021 gtagtccatc gacagcccga agaggacgca gaacaggatg accggctggg tcgtctcgac
     1081 gaacccgagc ggctggaaac cgagcagcgc cgagaggttg ccgtcctgga agatccagac
     1141 gagcgcgccg aagctcgcga cgatcgagag cgtgttcatc acgagcgcct tggccggcag
     1201 cacgaccgag cgcaggagca tgaacaggac gagataggtc gtgacgacga tgaagagccc
     1261 tgtccgcggg aagtcctggg ccacccgacc gacgacgtcg ctgacgtctg ccgcgccgcc
     1321 gccgacgagg acggtcatcc cggccggcgg cgcgagcggg cccgccgggt cgcgcagggc
     1381 ggtgaccagc gagcggccct cgtcgcgatt ggggccgaac ggcgtcgtga tcgtgaacgc
     1441 ggtgaggttg ccgttggtcg tggctgcgag ggcggttgcg acgaaccggt cgcgcggccc
     1501 gttcgggtcg gcgtagagca gctggtactg gtccttgcgg agacgcgggt cgacatcgac
     1561 gaacgagtcg atccggcgga tccgcggatc ggccgcaagc cgccgcgagt agtcgtagag
     1621 cgccgcgacg ttgtcggtcg acgtcgccgc gccagcagtc cggatcgcga ggacgatcgg
     1681 cgcgaactcg ccttcgccga acgcgttcgc caggcgatcg aaggcggccc gcgacgggac
     1741 gctggccggc aggatcgtcg agtccggggc gttgaaccgg acgtgcagga agggcgagcc
     1801 gagcaccagg aggaccccga gcgtcgggac gagaacggcc accgggtggc gcatcacccg
     1861 gcgggccagc cgtgcccacg gtccgttcgg gtcgtcgcgt gggatcaccc ggcggatcgc
     1921 gagggcgtcg atccgcggtc cgacgatcgc cagcagggca ggcagcagcg tgagggccga
     1981 cgacgcggcg agcaggacga cgatcgcgcc cgcgatcccg acggagcgaa ggatcatgaa
     2041 ctcgaacagg acgaggccga tcagcccgag gagcacggtc agacccgaga agaacacggc
     2101 tcgcccggcc gtctcgaccg tcgcctcgac ggcgccggcc acccgctcgg ccggtggatc
     2161 ggcagcccgg gccgccatct cctcgcggaa gcggctcgtc atcaggagcg agtagtccac
     2221 cccgagaccg agcccgagca gcgttgcgag gttgaggacg aagatgctca tcggggtcac
     2281 gcttccgacg aggaagatcg ccgccagggc gaccacgacc gccgccccgc cgacgatgag
     2341 cggcagcgcg gccgccgcga ccgagccgaa cacgagcagg agggcgagcg cggccagcgg
     2401 cagcgacacg acctcgctcc ggcggaggtc ggattccgag accgattgga cgtcgccgta
     2461 gaaggccggg ccgccggcca gctggacctc gagcccggcg ggctgggcaa gccgctcacg
     2521 gatcccgggc agtgcggcgg gcgaatcgtc gggcgcgatc gacatgaaca cgatgtcgta
     2581 ggcggtgtgg ccgtcggtcg agacctggcg cgggctcagg gtgtgcggca ggatccggac
     2641 aacgtacggg gcctccggga cgtgctggat ggcagcggcc gccgcggcct cgaacggcgg
     2701 cgttccggcc gttccgacag cgctgtggta gacgacgacg accgccgacg gcgggctcgc
     2761 cagctcggcc tggaggagcg ccttcgcccg cgccgactcg agatcgtcga ggatgaaacc
     2821 gccggcgcgg aggacgccga ccacgcgcgg agcgaggggg atcgcgacga ggaggatgac
     2881 cgcccagacc aggacgatcg cccaccggcg gcggacggcg aaccggccga gacgagcgaa
     2941 gggatcggag gcggaccgac gctcggcccg ctgaccggtc cctcgtcgcg agcgttcgtc
     3001 gccgggttcg cggggcacgg acggaccgat acgtcctttt ggggaggtcg acatgcgtgc
     3061 gctcatgcta tacagacaac cggatcacgc atcccgtgtg ggagctgggc gggcgcccga
     3121 cggagagatg accatcgacg cctcagagcg cgatccgcgc gcacgcgctg gatcgaccga
     3181 cgcgaccggg gactgggctg ccatctcggc atgggcccgg gatctcgccg cacgcatcgg
     3241 cagcgagcag ccgacccagc acatcgcgat cttcgtcccc gacgacgatg gcgggcgact
     3301 ccgcctggcc gcccaggtct ggggtgccgg cgaggacacc ggtgaggtga tcgtcggcca
     3361 atggctcgtg cccctcgagg gctcggtctg cggtcgggtc tttcggaccg ggctggcggc
     3421 cctctgcgcc gacgtcggga tggaccccga ctaccgcgcc ttcccgggcg ggcggacgcg
     3481 atcatccctg accgtcccgg tcggtccgcc cgacggggtc gcggcggtca tcaatctcga
     3541 ggcgccgtgg gtcggggcgt tctcgatccg tgactacgag cggatgaccg aagaggccgc
     3601 cgcggcatac gcatcgttcc ccgtccggcg cgtcgcctga ccgacggcca gcccccgcgt
     3661 ccgaggcttc ccgagatccg gacgcgcacg ccgaggccct gaccgcgccc ggccatctgt
     3721 tagcatctgc cggcacgtcc gccagccaca gcgaggcccc cggtgcagct gttcacgatc
     3781 ttcttctcgc gacccgtcga gttcggcgtt gccaccgcga tcaccgtcct gatgctggtg
     3841 atcctgctgc gggcggcgat ctcgtcggag ggtccgtcgg gcctcggccg gttcatggca
     3901 cggcccacgt cgaagttcgt tttcggcttc ctgttcctcg cctgggccgt cgtgttcggg
     3961 atcggcctcc agctcgttcc gcacgagggg gccaactcgc cctacggcgg tctcggcctg
     4021 gtcgcgatgt tcagcggttt cttcatcatg atgggcttca tctggtcggt gatcggcgag
     4081 tagcgcgagc ctgaccgggc gtgcgatcac gacccggcca cgcgacctgg atggtacggt
     4141 cctcgcggag tccgcctctg gtgcgggcga gaggggagca cgccgatgac cacgagccgc
     4201 ctccgttcga cgagccaccg cccgaccgca cgccgcggca cgatccgggc ggccgccacg
     4261 gcgatcgcat cggccgcgat cctgctggcc ggggtcctac cgacatccgt ggccgctgcg
     4321 ggcgacctga ccccgccgac cggcagcgtc gagtactgga caatcgacgc cgacacgcag
     4381 ctcgccgaat tccgcttcca ttacacggat ccggagagcg gcctcgacca catcaccgtc
     4441 gtgtgcgacg gctccgctcc gatcacgatc ccgtactcgg ccaccgtcct gtgggcgatc
     4501 cacgatggaa ccggcgggtg cacgaccgcc tacggcgatc acgacgtgtg gatcgaagtc
     4561 ttcaacggag acggcctcgg agcccccggc acgggtttca ccgtcacgaa cggcccgagc
     4621 ctgcatctca cggtctccac cgcggtcacc ggccacccga tcacgatcac gcccgtcttc
     4681 ccgtccgact tcacggtccc cgccggcgac ggctgccgct gggagttccg atggggcacc
     4741 aaccgggcgc tcgatcaggg tttcgcggac gagacgttcg gcggcctgct cttcgatgtt
     4801 cgtgcctccg acggcggctg tggtccatgg acgttcacgt tgccgtgggt cccttaccgc
     4861 cagttcgacg tgcgggtcga cgtcttccac caggatcccg acggcgggtt cgtggagggt
     4921 ccgggtgcgc agcaccgatt cacggcggcg gccgacggca cggaccgccg agtcctgacc
     4981 tcgaacctgc cgatcgcgca ggtgttgccg agcacgtaca caccgatcgt cgggacgccg
     5041 gtcacgtaca cgcgctacct cgtcggtggg gccaccgtgt gctgcaacgc caactgggtg
     5101 gcccgcctcg gcaacggcga gaacccgatc gtctggaccc agaacggcgg atcgacgttc
     5161 accttcacgc catccgcgac ggggaacctg ctcgtgagct gggcacgcga aggctcgacc
     5221 ggcctcgtgc tgtccggcta ctacgacccg ccggtccgct accgcgacac gacggcaccg
     5281 gtcgcgaccg cgcctcgtga gcggatccgc ccgatggcgg ccagcgagac tgtcccgatt
     5341 gcgatcgagt ggggcgggac ggatcgcggc tggggcatcg cctccttcca gctccagcgg
     5401 agcctgaacg gcggcggctg gatctcggtg acgctgccga gcgtgaccgc cacgtccgtc
     5461 ggcatcaacg gggtcgttgg cacgacgctg cgcttccggg tccgggccaa ggacaaggcc
     5521 ggcaatgtcg gcagttgggt ctacggttcg acgttccggg tcagccgcgt ctccgacgcg
     5581 agcacgacga ttcgctactc caccggctgg acggcgaccg cccgccccgg cgcatacggc
     5641 gggatgctcc acgcgacggc cggcgcgggt gcggcgatga gctacacgtt cttcggacgg
     5701 gacgtggcct ggatcgcgtc gcgcggcccc gactacggcc gggcgaagat ctacctcgac
     5761 ggcgcgtaca tcgggagcat cgacctgttc gcgacctcgc ccgaggcgcg ccggatcgtg
     5821 ttcgcccgtc actggacgag cgtcgggacg cacaccctcc ggatcgtcaa cgtggcgacg
     5881 agcggccggc cctggatcga cgtcgacggg ttcgctgtcc tccgctgaca ttcgaaacgc
     5941 aggcaacagg acgcggctgt tctcgagacc tggatcgccg gactgcccga cgccctgctc
     6001 gaccctgccg aggcggccga catcgcgctc accgatcccc gcttgaccgc ggtgctcgcg
     6061 agccgcgatc tccggaacgg caacgagggg gtgctgcggt tcgatccgcg cacgggcacc
     6121 tatcaggtcg ggatgttgga gtcgggcagc ctgccggtgt cgcgggctca cctcgtgctc
     6181 gtgaacgcgc ggaccggcga catcgtcggg ttcgtcgagc gggactggga ctacgccgtc
     6241 gacggcttcc cgtcggcccg ggccgggact tccggccctc tggaccgccc gtgacgcgcc
     6301 gcgctatgat ccgctgactt gcagactgca tagactttat gcgttcgccg cgctcgagtg
     6361 gccggcgacg cgcaccagga ggccgcccga gttggacgcc gacgagatca agaccgcggt
     6421 tgcccgccag gctgccgagc gcgccgccgc cgagctgccg ttcgtcaatc ggcgcgaccc
     6481 cgcccagatg ctcgggcccg acggccgtcc cgccgtcagc cgccgcgcgc cgatctggga
     6541 ggacgtcccg gacgagaagt ggaacgactg gcgctggcag ctgagccatc gagtcaacga
     6601 tctcgaggag atcgagcaga tcctcaacct gaccgaggat gagcgtgagg gcctgtcggc
     6661 gaaggacaag ttccgggtcg acatcacgcc gtacttcatc tcgctcatcg accccgacga
     6721 cccgaacgat ccgatccgcc gccaggtgat cccgctcggg cgggagcagc aggccttcac
     6781 cgcgatgatg gaggactcgc tcgccgagga ccgccactcg ccggtcccgg gcctcgtcca
     6841 tcgctacccg gatcgcgtcc tgatgctcgt caccacccag tgcgcgagct actgccgcta
     6901 ctgcacccgg agccggatcg tcggcgaccc gacccagaac ttcaaccgca aggaccacga
     6961 ggcccagctc gactacctcc gccggacccc gcaggtccgc gacgtcctga tcagcggcgg
     7021 cgacggcctg accctcgccc cgaagctgtt cgagtcgatc ctgcgcggcc tccgcgagat
     7081 cccccacatc gagatcatcc ggatcggttc gcgcgtgccg gtcttcctgc cgcagcggat
     7141 cgacgacgag ctctgcgcga tgctcgagaa gtaccacccg ctctggatca acctccattt
     7201 caaccacccg aacgagatca ccccggaggt gtcgcgcgcg gtcgacaagc tgacgaaggc
     7261 cggcctgccg gtcggcaacc agagcgtcct gctcgccggc gtcaacgact gcgtccacat
     7321 ccagcgctcg ctcgtccaca agctcgtcga gaaccggatc cggccgtact acctctatca
     7381 gtgcgacctg gtcgagggct ccggtcactt ccggacgccc gtcggcaagg gcctcgagat
     7441 catggagggg ctgcgcggcc acacctcggg ctacgcggtt ccgacctacg tgatcgacgc
     7501 gcccggcggt ggcggcaaga tcccggtcat gccgaactac ctgatcagct acagcgatca
     7561 caaggtcgtg ctccggaatt acgagggcta catcacgacc tacgaggagc cgccgaccta
     7621 cgaacgccac gacgaggcgg cctgctccta ttgccagagt cagcgctccg agcccggcca
     7681 gtcgggcgtg ctgggcctgc tccagggcga gcggatgtgg atcgagccgg ccggcttcga
     7741 actgacccac agccgcggca acacccaggc ccaccgcctc caggacccgg caaaatgggt
     7801 gccgttcggg gtcggggcga tcgagggcca ggcgacccgt ccgctccgcg tcctcgaggc
     7861 cggcgagcac ttgctcgacg acttcgatcg ggcggcgacc gagaccaacg ccggaatcgt
     7921 cgagccgggc gatctcgagt cggaggagga gcgggccgcc cacgcccgtg acgccgcccc
     7981 ggcctactcc cccggggagg agccgcgccc gcgcgacggc gagccgaccg ggtcggccca
     8041 cggcgaactc ctctaggcgt cgccagcacc gggctcccgg cgggcgcgct gaaaggttgc
     8101 cagtgcctcc gcctgccgac cgctccggta ctgggcgagc atcagctgcc gccagaggcg
     8161 ttcccggaac ggctcttcgc gcacaagttc ctcgatctcg acgatcaggc cggcggtcga
     8221 tccggcgtcg aggtcgtggt cgatccggtc ttcgat
//
LOCUS       DATAIW010000381         5832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_174387, whole genome shotgun sequence.
ACCESSION   DATAIW010000381 DATAIW010000000
VERSION     DATAIW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174387"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VHM48_11400"
     CDS             <1..1128
                     /locus_tag="VHM48_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756064.1"
                     /translation="AAVLLITRIRAAVEARRPRPIAQLIGLGVVLGLAALTRNEAAWL
                     ALAWVAFAWLLPVARRERIVLIAVPAVVALAIFAPWMIRDWLAFGSPLPGQALTNALS
                     IDGTDIFAWSDPPTVGRYLAVGAGRLVDMRVIGIEHNLFEVLLLPGAPLSVIGAVALP
                     WMLRTRALQPLVAVSVAIFLVTSLLFPVSTTWGTFLHAAGAVHVLLMISALLALDRLI
                     VAIGRRRGWTRPVAWLAPALTVSGALLFSAVLLPSFGAGSVSTERTFEALDRQMTAAG
                     LPIDGPGPVITDAPIWLPYVAGGTALALPFEPAASVLDLANRFRSTTVIVLSRDHPFG
                     ATLAAGGPATECFVPVDIGVPADPVAATALTGVRVFRVVCP"
     gene            1152..2273
                     /locus_tag="VHM48_11405"
     CDS             1152..2273
                     /locus_tag="VHM48_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2756065.1"
                     /translation="MDAARSSDDIRTVRDVRIDGLRAEASEAVGYSANTLRTVSQRYR
                     TTYADEFSRWQALRDELDAIERGRPESHGHRPDYEDRGPDEAAISAAEAGAEDARVRA
                     LRSDVDTVGRELGRQQTELAKLEIALRSLETTWLFLERGDASLISDGEHVPTDVQMRI
                     VEAQESERSRLAQEVHDGPAQTLSNAIFQVEYIERVIETDLRAARTELRFLRDLLRRE
                     LGSVRTFISQLRPPVLDELGLDGAISDAIGRTTALTGLAISSDLTASPDRLTGTQQTV
                     VLRILQEALQNVRKHGAASAVTVASAIDGGDWVLTVRDDGRGFDVGAVAARGRRNFGL
                     QFMRERAELISAAFEVHSRPDGGTLVRLAIPVGAEETSS"
     gene            2270..3007
                     /locus_tag="VHM48_11410"
     CDS             2270..3007
                     /locus_tag="VHM48_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756066.1"
                     /translation="MSYSGQERRRGGPDRRADGERTRILIVDDHALFRVGIANILGRE
                     SDFEVVGEADDSRTAIDRALETSPDIILMDLSLPPPGGIETTQRIKRELPSTGIIVLA
                     ATEDEDALFDSIKAGAAAFILKDVGPDDLVTIIRRVVNGEYLINDKVFSKPAVASRVL
                     KEFRELAVYGQEAAPIFAPLSPREVEILDNIAQGMTNKQVAYALSISEQTVKNHMSSI
                     LRKLSVNDRTQAVVYAMRQGWIRMPED"
     gene            3017..4285
                     /locus_tag="VHM48_11415"
     CDS             3017..4285
                     /locus_tag="VHM48_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2756067.1"
                     /translation="MSSSQRNSTAASTGGGGDLAARLGTEIAALDSELTEIEMLVAQA
                     QSEATRHEQRRVQTTEKLATGVNLPPADLVALNTQLVSLTRRAAVMEAQVEVLEGKRK
                     TLARFRDSLIDIAEAFGAALPVEGSGSGGSAGSGGSGGPGGSGGHTAPRLTAGEGTGS
                     PMSRIVLNAQEDLRREIARAMHDGPAQSLTNIVLQAQIVERLLGRDPDGAHGELRLLV
                     SMVQQTLEATKSFIFDVRPMVLDDLGLVPTLRRAARERGRRAGISVEFDSVGADRRID
                     VDLESSLFRIIDEALTGYLSGRPDRIAIRLDWSEDAVEARVNAERDPTKQMADADHEV
                     AEAVKAVAATDKDLPPALESMMADRKERAEARSVAAREAAIVALPVSTWREIQQRATT
                     TGIVAELSDGGGTLHVRVDLETVGGADEAS"
     gene            4266..4484
                     /locus_tag="VHM48_11420"
     CDS             4266..4484
                     /locus_tag="VHM48_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756068.1"
                     /translation="MLTKRPDRALVAGGLAWARAGRALNDRRGQGVVEYGLILSLSAG
                     LAVILLVFFGGTLSAVLKAVGDAIDAAS"
     gene            5358..5528
                     /locus_tag="VHM48_11425"
     CDS             5358..5528
                     /locus_tag="VHM48_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756069.1"
                     /translation="MALIHQFLASFRRDEEGQGLAEYALILALIAIVAIIALIFLGGQ
                     VSKILSTVGSSV"
BASE COUNT          834 a         2071 c         2046 g          881 t
ORIGIN      
        1 gccgcggtcc tgctgatcac gcggatccgg gccgcagtgg aggcccgccg accgcgcccg
       61 atcgcgcagc tcatcgggct cggcgtggtc ctcggcctgg ccgccctgac ccgcaacgag
      121 gcggcctggc tggccctcgc ctgggtcgcc ttcgcctggc tgctgccggt cgcgcggcgc
      181 gagcggatcg tcctgatcgc cgtgccggcc gtcgtcgccc tcgcgatctt cgcgccctgg
      241 atgatccgcg actggctcgc gttcggcagc cctctgccgg gccaggccct gacgaacgcc
      301 ctgtcgatcg acgggaccga catcttcgcc tggagcgacc cgccgaccgt gggccgctat
      361 ttggcggtcg gggccggccg gctggtcgat atgcgggtca tcggcatcga gcacaacctg
      421 ttcgaggtcc tgctcctgcc gggtgcgcca ctctcggtca tcggtgccgt ggccctgccg
      481 tggatgctcc ggacgcgggc cctccagccg cttgtggcgg tctcggtggc gatcttcctc
      541 gtgacgagcc tgctcttccc ggtctcgacg acatggggca cgttccttca cgccgcgggc
      601 gccgtccacg tgctgctgat gatctcggcc ctgctcgcgc tcgaccggct gatcgtcgcg
      661 atcggccgcc gccgcggctg gacccggccc gtcgcctggc tcgccccggc gctgacggtc
      721 tcgggcgccc tgctcttctc cgccgtcctg ttgccgagct tcggcgccgg ctccgtctcg
      781 accgagcgga ccttcgaggc gctcgatcgc cagatgaccg ccgcgggcct gccgatcgac
      841 ggacccggcc cggtgatcac cgacgcgccg atctggctgc cgtacgtcgc cgggggcacc
      901 gcgctcgcgc tgccgttcga gccggcggcc agcgtcctcg acctcgccaa ccggttccgc
      961 tcgacgacgg tcatcgtcct cagccgcgac cacccgttcg gggcgaccct cgcggccggc
     1021 gggccagcca ccgaatgctt cgtgccggtc gacatcggcg tgcccgccga tcccgtcgcg
     1081 gcgacggccc tgaccggcgt gcgggtcttc cgcgtcgtct gcccatgacc ggcccgtata
     1141 ctccggggcg aatggacgcg gccaggtcca gtgacgacat ccgaaccgtc cgcgacgtgc
     1201 ggatcgacgg tctccgcgcg gaagcgtcgg aggcggtcgg ttacagcgcg aacacgctca
     1261 ggaccgtcag ccagcgctac cggaccacct acgccgacga gttctcccgc tggcaggcgc
     1321 tccgcgacga gctcgacgcg atcgaacgcg gccgacccga gtcccacggc caccggcccg
     1381 attacgagga tcgcggcccg gacgaggcgg ccatcagcgc ggctgaggcc ggcgccgagg
     1441 atgcccgggt ccgtgccctg cggagcgacg tcgacacggt cggtcgcgag ctcggccgcc
     1501 agcagaccga gctcgccaag ctcgagatcg cgttgcgcag cctcgagacg acctggctct
     1561 tcctcgaacg cggcgatgcg agcctgatca gcgacggtga gcacgttccg accgacgtcc
     1621 agatgcggat cgtcgaggcc caggagtcgg agcgctcgcg cctcgcccag gaggtccacg
     1681 acggcccggc ccagacgctc tcgaacgcga tcttccaggt cgagtacatc gagcgcgtga
     1741 tcgagaccga cctgcgcgca gcccggacgg agctgcgctt cctgcgcgac ctgctccgcc
     1801 gcgagctcgg ctcggtccgg acgttcatca gccagctccg gccgccggtc ctcgacgagc
     1861 tcggcctcga cggcgcgatc tcggacgcca tcggccggac cacggcgctc acggggctgg
     1921 ccatcagctc cgacctgacc gcctccccgg atcggctgac cggcacccag cagacggtcg
     1981 tcctgcgcat cctgcaggaa gcactgcaga atgtacgcaa gcacggcgcg gcctcggccg
     2041 tcacggtcgc gagcgcgatc gacggtggcg actgggtcct gacggtccgc gacgatggtc
     2101 gcgggttcga tgtcggagcg gtcgcggctc gtggtcgacg caacttcgga ctgcaattca
     2161 tgcgagagcg cgcggaactg atcagtgccg ccttcgaggt gcattcacga ccggacgggg
     2221 ggaccctcgt ccggctcgcg atcccggtgg gagcagagga gaccagctca tgagctattc
     2281 gggacaggaa cgtcgccgag gtggaccgga tcgacgggcc gacggcgagc ggacgcggat
     2341 cctgatcgtc gacgaccatg cccttttccg cgtcgggatc gccaacatcc tcgggcgcga
     2401 gtccgacttc gaggtcgtcg gcgaggcgga cgacagccgg acggcgatcg atcgggcgct
     2461 cgagacgtcg ccggacatca tcctgatgga cctctcgctg ccgccccccg gcgggatcga
     2521 gacgacccag cggatcaagc gcgagctgcc ctcgaccggg atcatcgtcc tcgccgcaac
     2581 ggaggacgag gacgccctgt tcgactcgat caaggcaggc gccgcggcgt tcatcctcaa
     2641 ggacgtcggc ccggacgatc tcgtcacgat catccgccgg gtcgtcaacg gcgagtactt
     2701 gatcaacgac aaggtcttct ccaagccggc cgtcgcgtcg cgggttctca aggagttccg
     2761 tgagctcgcg gtctacggtc aggaggccgc cccgatcttc gcgccgctct cgccgcgcga
     2821 ggtcgagatc ctcgacaaca tcgcccaggg catgaccaac aagcaggtcg cctacgccct
     2881 ctcgatcagt gagcagaccg tcaagaacca tatgagctcg atcctgcgca agctgtcggt
     2941 caacgaccgg acccaggccg tcgtctacgc gatgcgccag ggctggatcc ggatgccaga
     3001 ggactgatcg gccgccgtgt cctcatccca acgcaattcc accgccgcct ccaccggcgg
     3061 cggtggtgac ctggccgccc ggcttggcac ggagatcgcg gcgctcgaca gtgagctgac
     3121 cgagatcgag atgctcgtcg cccaggccca gtccgaggcg acccgccacg agcagcggcg
     3181 ggtccagacg accgagaagc tggcgacggg cgtcaacctg ccgccggcgg acctcgtcgc
     3241 cctcaacacc cagctcgtgt cgctgacccg gcgggcggcc gtcatggagg cccaggtcga
     3301 ggtgctcgag ggcaagcgca agacgctggc ccgctttcgc gacagcctga tcgacattgc
     3361 cgaggctttc ggggcggcgc tgccggtgga gggcagcggg tccggcgggt ccgcggggtc
     3421 cggcgggtcc ggcggccctg gcgggtccgg cgggcacacg gcgccgcgcc tgaccgccgg
     3481 cgaaggcacc ggctcgccga tgtcgcggat cgttctcaac gcccaggagg acctccgccg
     3541 cgagatcgcc cgggcgatgc acgacggacc ggcgcagagc ctcacgaaca tcgtcctcca
     3601 ggcgcagatc gtcgaacgat tgctcggccg cgatcccgac ggggcccacg gcgagctgcg
     3661 gctcctggtc tcgatggtcc agcagaccct cgaggcgacg aagagcttca tcttcgacgt
     3721 ccgcccgatg gtcctcgacg atctcggcct cgtcccgacg ctccggcggg cggcgcgcga
     3781 gcgggggcgg cgagccggga tctcggtcga gttcgactcg gtcggggcgg atcggcggat
     3841 cgacgtcgat ctcgagagca gcctcttccg gatcatcgac gaggccctga cgggttacct
     3901 ctcggggcgg cccgaccgga tcgcgatccg cctcgactgg tccgaggatg ccgtcgaggc
     3961 ccgcgtcaac gccgagcgcg acccgaccaa gcagatggcc gacgcggacc acgaggtcgc
     4021 cgaggcggtc aaggccgtcg ccgcaaccga caaggacctg ccgcccgcac tcgagtcgat
     4081 gatggcggac cggaaggagc gggcggaggc caggtcggtc gcggcgcgcg aggcggcgat
     4141 cgtcgctctg ccggtctcga cgtggcggga gatccagcag cgggcgacca cgacggggat
     4201 cgtcgccgag ctgagcgacg gcggcgggac cctccatgtc cgggtggacc tcgagacggt
     4261 cggtggtgct gacgaagcgt cctgaccgcg cgctcgttgc cggcggcctc gcctgggcgc
     4321 gcgccggccg cgccctgaac gaccggcgcg gccagggcgt cgtcgaatac gggctgatcc
     4381 tgtcgttgtc ggccggcctg gccgtgatcc tgctcgtctt cttcggcgga acgctgagcg
     4441 ccgtcctgaa ggcagtcggc gacgccatcg acgccgcctc ctgaggggct gtcgcgcctt
     4501 cgcggccgat cgcggcgttg acccctgcgg tcctcgctca cgggcatccg agcctgctcg
     4561 ctgcggtcct cggggtcgcc ttgcgctcga ccaggcatgc gcgacgaacg cagtcgcgtg
     4621 gcgatgggcg ggttgtcgcg ccttcgcgac gaacgccacc ggccgagcaa gccggacccg
     4681 accgcctgcg ctcaggcgag gctcgcctgt tgtcgcacgg acggcgcccg atttcttgcg
     4741 ctcaggcgac gctcgcctcg ggcgagcgtc ctggcggatg ggctgagcgt ggcgtgctcg
     4801 cgaccaagca ccgatctcgc gtgcaacagg cgacgctcgc ctgggcgcaa aggaaacgac
     4861 cgacgccggt caagctcacc gcgcagccgc gcgacgtggg acgcacgatc aggtggcctt
     4921 ctcggcgggc gcgttgcgtg gggcgcacgt gtcgggggtt cgatggccgg tcgagcgcgg
     4981 agatcgagcg gttcgttgtg cccggcgaaa cgaaacgcgc gggcacgggc ggcgatgccc
     5041 ctcgacgggg tcggatgttg cgccggccga aggggaacga ccgctcggcc tggtctggct
     5101 cgcgaaaccg tgcgacgtgt gcgtccggcg caacgcgttg gcgggatctt gcgtgggatg
     5161 cacgatcgtg acgggatggc ttccgcgacg gcccggggag gcggaggatc ggcgatggtg
     5221 cgcatccggt ccgcgtctgc accggtccgt cagaggcttg tcattggtac gaataggcta
     5281 gtgcagttcc cggggcctct ctgcaaagat gacgcagccc tcactcgggg gtgggtactc
     5341 atccaggagg cagccgcatg gcgcttattc accagttcct cgcctcgttc cgccgggacg
     5401 aggaaggcca gggtctcgcg gagtacgcgt tgatcctcgc cctcatcgcg atcgttgcga
     5461 tcatcgccct catcttcctg ggcggccagg tcagcaagat cctcagcacg gtcggcagct
     5521 cggtctagtc cggagagcca ccggctcaag caacgaaggg gccgttggac agactgtcca
     5581 gcggcctctt tgcttccgca ggacgttcag ctcgggccgc gctttgcgca ccgtgcacgt
     5641 cacgctacga taggggtcga tccacgaccg ggggcacggg tcgccgatca gatcgagcac
     5701 aggcatcggc cgagtgctca tgagcaaagg agggcgtcag cccaatgtcc attcttcaca
     5761 cggtcttcgc ctcgcttcgt cgggacgagg aaggccaggg tctcgcggag tacgcgttga
     5821 tcctcgccct ca
//
LOCUS       DATAIW010000382         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_85197, whole genome shotgun sequence.
ACCESSION   DATAIW010000382 DATAIW010000000
VERSION     DATAIW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85197"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1993)
                     /gene="recJ"
                     /locus_tag="VHM48_11430"
     CDS             complement(152..1993)
                     /gene="recJ"
                     /locus_tag="VHM48_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX2756070.1"
                     /translation="MIEPRFAWRVAEAVTPTVDLLEAGARLGLGLRAIGLLAGRGLTT
                     VDELDAFFAEPMTALHDPRLLPDAEAFLTRLRAARSDGEKVMVFGDFDADGLTGLAIL
                     VRALRAWGMDPLPYVPSRLDEGHGLSDKALDAAREAGVTLIVTVDCGSTSLPEIAAAR
                     ASGIDVLVTDHHRLPPVLPVALAVVNPQRADSRYPERRLAGSGVAFKLAQLLLADEPG
                     GPAAALELCDLATIGSIADLVPILGETRAIVRLGLARIAARPRPGIAALLAAASVVPS
                     AVDIETLSFAVAPRINAAGRVGESAAAAALLMTDDREEADRLAAELETANRTRRDLTS
                     QAMGEARAAIAAIDPASIAGTGGLAAEAPADPVAIHATIVRGPWPVGIVGLVASRLVD
                     DHGRPAIVGADLGDTIRASCRSDGRLDIAATLDACGPIFLRHGGHAGAAGFEIEAGRW
                     PEFIATFDALAARAIPNDPRLPLAIDFALPALEVDYALHRDLRRLDPCGQGNPEPLLI
                     VQGLTVTRVRAASGGHTQLTLRRRLDVLDGIAFGRPDLAETVHDGDRVDVVARLTSRT
                     FGGYESLQLDIRDVASSGLHDAAWKLDARAVDGTPAVTGSLALPVAS"
BASE COUNT          298 a          775 c          859 g          257 t
ORIGIN      
        1 gggatgttcc cgttcatcag gctcagggtg agcgcggcga tgacgacgag cgcgaccacc
       61 gcgacgatcg gtccgacgaa gcccgaaacc gggccgatgc cgtacgggtc gcgcggtgcc
      121 cgcggcgtcc gggccggcgc cccgttgcgg gtcacgaggc gaccgggagc gccaggctcc
      181 ccgtcacggc cggggtgccg tcgaccgccc gggcgtcgag cttccaggcg gcgtcgtgga
      241 ggccgctgct cgccacatcc cggatgtcga gctggagcga ctcgtagccg ccgaacgtcc
      301 ggctggtcag gcgagcgacg acgtcgacgc gatccccgtc atgcacggtc tcggcgaggt
      361 ccggtcgccc gaaggcgatc ccgtcgagga cgtcgaggcg gcggcggagg gtgagctggg
      421 tgtggccgcc gctggcggcg cggacgcggg tcacggtcag accctggacg atcaggagcg
      481 gctcggggtt gccctggccg cacgggtcga ggcgacgcag gtcgcgatgg agcgcgtagt
      541 cgacctcgag ggccggcagg gcgaagtcga tcgccagcgg caggcgcggg tcgttcggga
      601 tcgcacgcgc ggcgagcgcg tcgaacgtgg cgatgaactc cggccaccgc ccggcctcga
      661 tctcgaagcc ggcggcgccg gcgtggccgc cgtggcgcag gaagatcgga ccgcacgcgt
      721 cgagggtggc agcgatgtcg agccggccgt cgctccggca cgacgcccgg atcgtgtcgc
      781 cgaggtcggc cccgacgatc gccggccggc catggtcgtc gacgagcctg gacgcgacga
      841 ggccgacgat gccgaccggc cacggcccgc ggacgatcgt cgcgtggatc gcgaccgggt
      901 ccgccggcgc ctcggccgcg agtccaccgg tccctgcgat cgacgccgga tcgatggccg
      961 cgatcgccgc ccgcgcctcg cccatcgcct gactcgtcag gtcgcgccgc gtgcggttgg
     1021 cggtctcgag ctccgcggca aggcgatctg cctcctcgcg gtcgtcggtc atgagcaggg
     1081 cggcggcggc cgcggactcc cccacccgac cggccgcatt gatccgcggc gcgacagcga
     1141 acgagagggt ctcgatgtcg accgccgacg gcacgacgga ggcggcggcg aggagggccg
     1201 cgatcccggg ccgcggccgg gccgcgatcc gggccagccc gagccggacg atcgcccgcg
     1261 tctcgccgag gatcgggacg aggtcggcga tgctgccgat cgtcgccagg tcgcacagtt
     1321 cgagcgccgc ggccgggccg cccggttcgt cggcgaggag cagctgggcg agcttgaacg
     1381 ccacgccgct gccggccagg cgccgctccg ggtagcggct gtcggcccgc tgcgggttga
     1441 cgaccgccag cgccaccggg aggaccggtg gcaggcgatg gtggtcggtg acgaggacgt
     1501 cgatgccgga cgctcgcgcg gcggcgatct cgggcaggct cgtcgagccg caatcgaccg
     1561 tgacgatgag cgtgacgccg gcctcgcgag ccgcgtcgag ggccttgtcg gacagtccgt
     1621 ggccctcgtc gagccggctc ggaacgtacg gcagcgggtc catgccccag gcgcggaggg
     1681 cacggacgag gatcgccagg cccgtcaggc cgtcggcatc gaagtcgccg aagaccatca
     1741 ccttctcgcc atcggatcgt gccgcgcgca ggcgagtcag gaacgcctcg gcatcgggca
     1801 gcaggcgggg atcgtggagc gcggtcatcg gctcggcgaa gaaggcgtcc agctcgtcca
     1861 cggtcgtcag cccgcggccg gccaggagcc cgatcgcgcg caggccgagt ccgagacggg
     1921 cgccggcctc gaggagatcg acggtcgggg tgacggcctc ggcgacccgc caggcgaagc
     1981 gcggttcgat cacccgatca ggatgccacg ggtcggtcac ctgcgggtcg tcgttcgctg
     2041 cacggcggct tcacgcccgc tcaccgggcc ctgcacggcg acttcacgcc ggctgacgtg
     2101 cggcgcacag caccttcacg cggagccacc acgacccggc gcggccgatc aggagacggc
     2161 gcggcgcggc cggggagctc gtgacgaac
//
LOCUS       DATAIW010000383         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_130088, whole genome shotgun sequence.
ACCESSION   DATAIW010000383 DATAIW010000000
VERSION     DATAIW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130088"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VHM48_11435"
     CDS             complement(<1..932)
                     /locus_tag="VHM48_11435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2756071.1"
                     /translation="MASPKIGYAAMLEQFAPQEVVGYSADAEQHGFTGVMAADHFQPW
                     VPQQGEAAFVWNVMTAIGERTKGDVGPGVACPSFRMHPAIIAQASATLAAMYPGRHWL
                     GLGSGEALNEHIVAGYWPETPERIARMFEAIEIIKKLFGSRDKDVKHEGKFFKLESTR
                     LWTMPDEAPPIYIATAGPVTAKKTGQYAEGLITVGAPESKLEMIRAKCEEGCRDAGKD
                     PADFRYILQLHLSWAPTDEEAMANALLEWPNGGMKFPKQDIRSPLDFEQMAKLVRPED
                     FDGRLLISSDPDQHRAQIQRFLDLGFDQIYLHNVG"
     gene            complement(935..1381)
                     /locus_tag="VHM48_11440"
     CDS             complement(935..1381)
                     /locus_tag="VHM48_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2756072.1"
                     /translation="MEPAQPTRTGHALLDDRLRAFLDQPLFCALASVDPDGAPRQAVI
                     WYRLEDDGRILLNSRAGRRWPANLARDGRVALAITAPDGYTWLGLTGRVDAIDDDVER
                     AREDIVALAHRYYPDGPTASSIATFRSQPRITFRIAIDGVHDHLED"
     gene            complement(1439..2635)
                     /locus_tag="VHM48_11445"
     CDS             complement(1439..2635)
                     /locus_tag="VHM48_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2756073.1"
                     /translation="MRFLLGTKPHDGDQRLLWSRDVRFAHRLLVALPDCRSYELDAGI
                     VVIGAAGDLTDARAAEIASAVHSPAYGLVSLDPALPEGERHLVALSSAVQQSGLAERI
                     GMAQSSQMIQSKMLDYFFTLKNVHAVAFQPIVELGSGRLVEYECLFRPQMPGLQTSIS
                     SVVQAAVDTGRTIELDGFIVRIILGRVGALQAARRAANLPAIHVAINFTPASLLDGQF
                     EATAFATRVKDAGLDPRQITLECTEQQAVSEVGPLVKQVKAFRRLGFGFAIDDAGAGY
                     ASFSLIAALRPSVIKIDREIASGIARDDAKQALVEAFVSFGGRIGARLLAEGIERRAD
                     LAMLTTLGVDLGQGYLIGKPAAEPAEPRVMDTLRLDAARLAIGRRVREVHPRSGRRSF
                     PADRPA"
     gene            complement(2786..3496)
                     /locus_tag="VHM48_11450"
     CDS             complement(2786..3496)
                     /locus_tag="VHM48_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX2756074.1"
                     /translation="MSDATPGGIAATLTLADWRRSISSLYADVRRLAETDPAAALDHW
                     RAVREGLYRDHPQSPLPADRRATFRAQHFGHDPSLRFELAVEALEQPQRDGAADPDPV
                     RVSRLDFGSGASLAIDLPVSAGGAMAFRRFGAVTIPFRAGPRRLELYWMEGYAGGLFL
                     PFRDATNGAETYGAGRYLLDTAKSADLGGDRAAGTLILDFNFSFQPSCAFDPQWACPL
                     APPANRLDLPVRAGERLA"
     gene            complement(3534..3947)
                     /locus_tag="VHM48_11455"
     CDS             complement(3534..3947)
                     /locus_tag="VHM48_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756075.1"
                     /translation="MTAPIDETQPHASPDPAAPVAPSAAPVPSPAPSPAAAAPPSPDS
                     RPALRADPDRPADSGWREPAWFPPRADRERHRDRRSGTFTIVVGLVLIAIGAWYFLDG
                     TLGIALPRIRWGSLWPVVLIVIGGLILLRSFRRKA"
     gene            4161..5009
                     /locus_tag="VHM48_11460"
     CDS             4161..5009
                     /locus_tag="VHM48_11460"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX2756076.1"
                     /translation="MADTAEIGVFGGSGFYSLLDDVREIKVDTPYGPPSDSFFLATVG
                     GRNVAFLPRHGRRHTIPPHRINYRANVWAMRSLGVKAVISPCAAGSLQRHVKPGDFVL
                     CDQFVDRTYGREGTFFDGPIVTHVSSAEIYDPELRRVAAEVIREHGISFHERGTIVII
                     NGPRFSTRAESKWFSDAGWEVISMTQYPEAYLCRELGMAVVNIALITDYDAGVLEGTE
                     AVDARSVLEVFSQNAERIQKVVLDMIGRFPADLDALGARAALEHTRGDGHATSPDDIR
                     LFETGL"
     gene            5307..6146
                     /locus_tag="VHM48_11465"
     CDS             5307..6146
                     /locus_tag="VHM48_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2756077.1"
                     /translation="MTERLPIGVSIRAIRAEPGWWLESARRLDDAGYAGVWSWDHFMG
                     RGKVGAPVVESWTILAMAAATTSRVTVGTFVANVMNRHPALLARMASTLQIASGGRLV
                     LGIGIGGAPKEHAAYGMDFPEAPERVARLEEAVAVLRALWTGGPVTRASAYYPLADAT
                     AFPVPDPPPPIIIGGETPAGARLAGRLGDGWSTFDDNFERNLPLYLEALAAAGRPRDG
                     QQVIVGFQGDWLGESDIAETEWVREPRAAWERWHEVGADGAIVLARSTADIDALVDAA
                     ARW"
BASE COUNT          894 a         2146 c         2296 g          898 t
ORIGIN      
        1 ccgacgttgt ggaggtagat ctggtcgaag ccgagatcga ggaatcgctg gatctgggcc
       61 cggtgctggt ccgggtcgga cgagatcagc agccggccgt cgaagtcctc gggccggacg
      121 agcttcgcca tctgctcgaa gtcgagcggg ctccggatgt cctgcttggg gaacttcatg
      181 ccgccgttgg gccactcgag cagcgcgttg gccatcgcct cctcgtcggt cggcgcccag
      241 gagaggtgga gctggaggat gtacctgaag tcggcagggt ccttgccggc gtcgcggcag
      301 ccctcctcgc acttggcccg gatcatctcg agcttcgact ccggcgcgcc gacggtgatc
      361 agcccctcgg cgtactggcc ggtcttcttc gcggtgaccg gcccggcggt cgcgatgtag
      421 atcggcggcg cctcgtcggg catcgtccac agccgggtcg actcgagctt gaagaacttg
      481 ccttcgtgct tgacgtcctt gtcgcggctg ccgaagagct tcttgatgat ctcgatcgcc
      541 tcgaacatcc gggcgatccg ctcgggcgtc tcgggccagt agccggccac gatgtgctcg
      601 ttgagcgcct cgccgctgcc gagcccgagc cagtgccggc cggggtacat cgcggccagg
      661 gtcgcggagg cctgggcgat gatcgccggg tgcatccgga atgacgggca cgccacaccg
      721 ggtccgacat cgcccttcgt ccgctcgccg atcgcggtca tcacgttcca gacgaatgcg
      781 gcttcgccct gttgggggac ccacggctgg aagtggtcgg cggccatgac gccggtgaag
      841 ccatgctgct ccgcgtcggc cgagtagccg accacctcct gcggcgcgaa ctgctcgagc
      901 atcgcggcgt agccgatctt cggtgaggcc atcgtcagtc ctccaggtgg tcgtggacgc
      961 cgtcgatggc gatacggaag gtgatccgcg gctgtgaccg gaacgtggcg atgctcgacg
     1021 cggtcgggcc gtcggggtag tagcggtggg cgagagcgac gatgtcctcg cgggcccgct
     1081 ccacatcgtc gtcgatcgcg tcgacgcggc cggtcagccc gagccaggtg tagccgtcgg
     1141 gcgcggtgat cgcgagtgcg acgcgtccat cccgtgcgag gttcgccggc caacgccgtc
     1201 cggcccggct gttcagcagg atccgaccat cgtcctcgag gcggtaccag atcacggcct
     1261 ggcggggcgc tccgtcgggg tcgacggacg cgagggcgca gaacagcggc tggtcgagga
     1321 aggcccggag gcggtcatcg agaagggcgt ggccggtcct cgtgggttgg gcaggctcca
     1381 tcgctcgatg gtacgcgagg tcgccggagc aggccgccga ggccggtcgg aaccgtggtc
     1441 aggccggccg gtccgccggg aaggaccgcc gtccagatcg gggatggacc tcgcgaaccc
     1501 gacgcccgat cgcgagacgg gcagcgtcga ggcggagggt gtccatcacc cgcggttctg
     1561 cgggctcggc ggccggcttg ccgatcagat agccctgtcc gagatcgacc ccgagggtcg
     1621 tcagcatcgc gaggtcggcc cgccgctcga tgccctcggc gaggagccgc gcgccgatcc
     1681 ggccgccgaa cgagacgaag gcctcgacga gtgcctgctt ggcgtcgtcg cgagcgatgc
     1741 ccgatgcgat ctcccggtcg atcttgatca ccgacggtcg cagggcggcg atcaggctga
     1801 agcttgcata gcccgcgccg gcgtcgtcga tcgcgaaccc gaacccgagg cggcggaagg
     1861 ccttgacctg tttcacgagc ggtccgacct cggagaccgc ctgctgttcg gtacattcga
     1921 gggtgatctg gcgcgggtcg agcccggcgt ccttgacccg cgtcgcgaag gcggttgcct
     1981 cgaactggcc gtcgagcagg ctggccgggg tgaagttgat ggccacgtgg atggcgggca
     2041 ggttcgccgc gcggcgggcc gcctggagcg cgccgacccg accgaggatg atccggacga
     2101 tgaacccgtc gagctcgatc gtccggcccg tgtcgaccgc cgcctggacc accgagctga
     2161 tcgacgtctg gagcccgggc atctgcggac ggaagaggca ctcgtattcg acgagccggc
     2221 ccgagccaag ctcgacgatc ggctggaacg cgaccgcgtg gacgttcttg agcgtgaaga
     2281 agtagtcgag catcttcgac tgaatcatct ggctcgattg ggccatcccg atccgctcgg
     2341 cgaggcccga ctgctggacg gccgacgaca acgcgacgag atggcgttcg ccctcgggca
     2401 gggccggatc gaggctgacg aggccgtagg ccggagaatg gacggccgac gcgatctcgg
     2461 cggcacgggc gtcggtcagg tcgccggccg cgccgatgac gacgatcccc gcatcgagct
     2521 catacgagcg acagtcgggc agcgccacga gcagccgatg ggcgaagcgg acatcgcgtg
     2581 accacaggag ccgctggtcg ccgtcgtgag gtttcgtccc gagaaggaat cgcaccaggg
     2641 tccctgtgtt ccagagtcgc gcgcgcgccg gggcgaacgc cccggtcgtc tccgcgctga
     2701 tcgtacgccc gggtgtcggc gcatcggacc caccgacggt ccgggttcga cgtagtccgg
     2761 aagtcccggg tccgggtcgg cgacctcagg cgagccgctc accggcccgg accgggagat
     2821 cgagtcggtt cgccggcggc gccagggggc acgcccactg ggggtcgaat gcgcacgacg
     2881 gctggaagga aaagttgaag tcgaggatca gggtgccggc cgcccggtcg ccgccgaggt
     2941 cggcgctctt cgccgtgtcg agcaggtagc gcccggcgcc gtaggtttcg gccccgttgg
     3001 tcgcgtcgcg gaacggcagg aacaggccgc cggcgtagcc ctccatccag tacagctcga
     3061 gccggcgcgg accggcgcgg aacgggatcg tcaccgcccc gaagcggcgg aacgccatgg
     3121 ccccgccggc gctgacgggc aggtcgatcg cgaggctcgc gcccgagccg aagtcgagcc
     3181 ggctgacacg gactgggtcc gggtcggccg caccatcccg ttgcggctgc tcgagcgcct
     3241 cgacggcgag ctcgaagcgg agcgacgggt catggccgaa gtgctgcgcc cggaacgtcg
     3301 cccgccggtc cgccggcagg ggcgactgcg ggtggtcgcg atagaggcct tcgcggaccg
     3361 cgcgccagtg gtcgagggct gccgcgggat cagtctccgc gagccggcgc acatcggcgt
     3421 agaggctgct gatcgatcgg cgccaatcgg cgagggtcag cgtcgccgcg atcccgccgg
     3481 gcgtggcatc gctcaccgct cgagatcgcc gggagcgccg gccacgggcc gactcaggcc
     3541 tttcgtcgga acgaacggag gaggatcagc ccgccgatca cgatcaggac gaccggccag
     3601 aggctgcccc accggatccg cggcagggcg atgcccagcg ttccgtcgag gaagtaccag
     3661 gcgccgatcg cgatcaggac cagcccgacc acgatcgtga aggtgccgga ccggcggtcg
     3721 cgatgccgct cgcggtcggc gcgtggcggg aaccaggccg gctcacgcca gccactgtcg
     3781 gctggtcggt cgggatcggc ccggagcgcg ggccgggagt ccggcgaggg cggggccgcg
     3841 gcggcgggcg agggcgccgg cgagggcacg ggcgccgccg aaggcgcgac cggagccgcc
     3901 gggtccgggc tcgcatgcgg ctgggtctcg tcgatcgggg cggtcatcgg caggctcctc
     3961 tggcgcggga catcgtagcc gggtcgggat cgcgccggcc agccgcggac ggccgtcgcc
     4021 cgatcaggcg tgcgacaatg cccgccgaac gacgacggcg gatggaccgc cgttccccgg
     4081 gatccggacc gtgtcgcgcg gcgcgcggtc gacggtcctc gggcacgaat cagcacgaag
     4141 tgaggaggca ggcgacggtg gtggcggaca cagcggagat cggcgtcttc gggggctccg
     4201 gcttctactc cctcctcgac gacgtccgcg agatcaaggt cgacacgccc tacggcccgc
     4261 catcggactc gttcttcctc gcgaccgtcg gcggtcggaa cgtcgctttc ctgccccgtc
     4321 acggccggcg tcacacgatc ccgccccaca ggatcaacta ccgggcaaac gtctgggcga
     4381 tgcgctcgct cggggtcaag gcggtcatct cgccgtgcgc cgccggatcg ctgcagcgcc
     4441 acgtcaagcc cggcgacttc gtcctgtgcg accagttcgt cgaccggacg tacggccgtg
     4501 agggaacctt cttcgacggc ccgatcgtga cccacgtctc gtcggccgag atctacgacc
     4561 cggagctccg gcgcgtcgcc gcggaggtca tccgcgagca cgggatctcg ttccacgagc
     4621 gcggcacgat cgtgatcatc aacgggcccc gcttctcgac ccgggccgag tcgaagtggt
     4681 tcagcgacgc cggctgggag gtcatctcga tgacccagta tcccgaggcg tacctgtgcc
     4741 gcgagctggg catggcggtc gtcaacatcg ccctgatcac cgattacgac gcgggcgtcc
     4801 tggagggcac cgaggccgtc gacgcgcgct cggtcctcga ggtcttctcg cagaacgccg
     4861 agcggatcca gaaggtcgtg ctcgacatga tcgggcgctt cccggccgat ctcgacgcgc
     4921 tcggcgcgcg ggccgccctc gagcacaccc gcggcgacgg ccatgcgacc tcgcccgacg
     4981 acatccgcct gttcgagacg gggctctagc cgcaacgtgg atctggtcgc cagccgctcg
     5041 acccgcgacc cgtcggcgcc ggcccctgcc gcgccggtat ctggccagat actcaccacc
     5101 agactcttcg gccttgtttg ggtcccgaat cgcgccgcag ttagccgtcg gctgctaacg
     5161 ggaggcttca tcgatggcaa atcggtagac agatccggct caggagtccc ccgaggaata
     5221 tccggccaag aagcgggggt cggtagcggc cgcgacgccg cgatccgccg cggggtcctg
     5281 gtcacggacg ccggcgcgac cgaccgatga ccgagcgact gccgatcggg gtctcgatcc
     5341 gcgcgatccg ggcggagccg ggctggtggc tcgagagcgc ccgccggctc gacgacgcgg
     5401 gttacgccgg ggtctggtcg tgggaccact tcatgggccg cggcaaggtc ggcgctccgg
     5461 tcgtcgagag ctggacgatc ctggcgatgg ccgccgcgac gacgagccgg gtcaccgtcg
     5521 gcacgttcgt cgccaacgtc atgaatcgcc acccggccct ccttgcccgg atggcgtcga
     5581 ccctccagat cgccagcggt ggacgactcg tcctcgggat cgggatcggc ggggcgccga
     5641 aggagcacgc ggcctacgga atggacttcc cggaggcgcc cgagcgggtg gcccggctgg
     5701 aggaggcggt cgccgtcctc cgcgcgttgt ggaccggcgg cccggtcacc cgtgcctccg
     5761 cgtactaccc actcgccgac gccaccgcct tccccgtccc ggacccgccg ccgccgatca
     5821 tcatcggcgg tgagacgccc gccggggccc ggctcgccgg tcggctcggg gacggctggt
     5881 cgaccttcga cgacaacttc gagcggaacc tgccgctcta tctcgaggcc ctcgcggcgg
     5941 ccggtcggcc gcgcgatggt cagcaggtca tcgtcgggtt ccagggcgat tggctgggcg
     6001 agtccgacat cgccgagacc gagtgggttc gcgagccgcg cgccgcgtgg gagcggtggc
     6061 acgaggtcgg cgccgacggg gcgatcgtcc tcgcccgctc gacggccgac atcgacgccc
     6121 tcgtcgacgc ggccgcccgc tggtagcgtt ggcctcgccc acgggccgcc ccgggcgcgg
     6181 ggacgagccc ggtcgagccc gatccgtggc accatcgagc ctgcgatgac cgcc
//
LOCUS       DATAIW010000384         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_55255, whole genome shotgun sequence.
ACCESSION   DATAIW010000384 DATAIW010000000
VERSION     DATAIW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55255"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1077)
                     /locus_tag="VHM48_11470"
     CDS             complement(127..1077)
                     /locus_tag="VHM48_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756078.1"
                     /translation="MPRRPGAAIDLGSTSVHLLIARPERTGLVVLEDESAFLGLGAAV
                     DGPGALGAAGRTTLVATVASYVERARAHGALHPIVMGTEPLRRLSDALRIVAEVSARA
                     GEPLAVLEHEEEAMLTLVGLTAGRAVKGDLLVVDIGGGSSELVEIGPGRLARAAGVQV
                     GAGRLTRRIVDRDPPSVEHVAALYRAADEAFDWTPTRPPVEVVFVGGTASNLLKLLRR
                     VGSPDRGDELDRVALGIVRDIVTATPAEMLAATYGMREARIRLLAAGAAIIETVLDRV
                     GMASGRVVDTGIREGAIIAADRAGDSWRDRLDELAHGWIA"
     gene            complement(1122..1754)
                     /locus_tag="VHM48_11475"
     CDS             complement(1122..1754)
                     /locus_tag="VHM48_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756079.1"
                     /translation="MNARTSDAGEGSTAAAAPSSNGRNQGRRAGAITPAAALARHVEW
                     LEFALAAARSEETWRAGRLEKATKKSREKRTARLSEVRDEIAELAALVDAVHRLQAQG
                     TQRTGRGPGRPRGSGARRTRAAAPPKAANRASATGSAAATAADAAPAPAATTPATATP
                     AVATAERAVSATRKKASTTKARAAAAKPADKATARRGPRPAAGPSADGQA"
     gene            2148..2813
                     /locus_tag="VHM48_11480"
     CDS             2148..2813
                     /locus_tag="VHM48_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756080.1"
                     /translation="MILAGHHAGVPSPGPDVDIVVAADGPAFVGFLHELKACLALLTC
                     PPGGPTEILAAVTDRRQRPALRIILLNDPADVTGRLEALALGFDDALPASIDPEELTG
                     RALLLANGNRGVAGHERQIPVAPGVILDLVGRRVRRGGVDVHLRPKEFALLSVLASDP
                     GRVFSRTELVDRAWGPAYDGGSRTIDVHIRWLRAKIERDPARPSHVVTVRGTGYRLDP
                     FVD"
     gene            3005..4015
                     /locus_tag="VHM48_11485"
     CDS             3005..4015
                     /locus_tag="VHM48_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2756081.1"
                     /translation="MIERSRRLGAVMIMAGLLATACSSGATPSPSSSGGAAPSTGASA
                     AAPGSQAAALSGSVNIDGSSTVYPITEAVAEEFQKANKGVKVTVNFAGTGGGFKKFCN
                     GETDMNDASRPIKADDAGEGAGCKAKGIEWLELGIATDALSVVVSKDNSFVTCLTTAQ
                     LKTMWDQGSTAKSWKDIDPSFPDEPLKLFGPGPDSGTFDYFTEVINGKAKQSRSDYTP
                     SEDDNALVTGVAGDKDALGYFGFAYLEENLDKIKAVAIDSGGGCIEPSADNVNGGKYK
                     PLARPLFIYPAVSALKRPEFAAFVQYYLANVNTYVDETGYIEALPAVLDKSKADLAAA
                     LK"
     gene            4100..5011
                     /gene="pstC"
                     /locus_tag="VHM48_11490"
     CDS             4100..5011
                     /gene="pstC"
                     /locus_tag="VHM48_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX2756082.1"
                     /translation="MTAASRPLTRRRPMSERIIETLLLVAASVGVLTTIGIVIVLMIE
                     AVQFFGLVNPIDFLTGTVWSASILPFRFGVIPLVVSTLEVALLALIVAVPLGLLAAVY
                     LAEYATLRTRNIVKPILETIAGIPTIVLGFFALNFITPDLLKPLLPTIGTFNILSAGL
                     VVGLLITPLIASLSEDALRAVPRGLREGAFAMGATRFEVVRRVVFPAALSGVMASIIL
                     AMSRAVGETMAVVLAAGFNPKLTIDPRDQGGTMAAFIAQITSGDTPQDSIQFKSLFAV
                     ALVLFAITLLLNLISNRIVARYRNKYA"
     gene            5008..5910
                     /gene="pstA"
                     /locus_tag="VHM48_11495"
     CDS             5008..5910
                     /gene="pstA"
                     /locus_tag="VHM48_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX2756083.1"
                     /translation="MSALTTPATGSVPRFDPRIAKRRMTGTIFLGACIAAIGILLIAL
                     VALLVDVVSKGVPWLDIDFLTGVPSRRAATAGILPALVGSLEISLLTGLISVPVGVGA
                     AIYLQEYARDTFLTRTLRTNIANLAGVPSIIYGIFGLAIFVRALSLGRSPLAAALTLA
                     LLIMPIVIIATIEALRAVPDSQREGAYALGATRWQTVRGSVLPAAAPGIVTGVILAIA
                     RAIGEAAPLIVIGASAFLVFLPTPLQQGFSVLPIQVFQWSSRPQADFQGIAAATILVM
                     LFLTAVLNVVAIVVRNRLARNIQW"
BASE COUNT          826 a         2231 c         2010 g          956 t
ORIGIN      
        1 gtcccgggga cgagcgacga gatcctcgtc tcggggctcg ccacccactg agcatcccaa
       61 gcaccgtccg agcccggtcg gctgaccccg accgggctcg ttcgattcag ccggtgcgtg
      121 cgaacctcag gcgatccagc cgtgggcgag ctcgtcgagc cgatcgcgcc agctgtcgcc
      181 ggcccgatcg gcggcgatga tcgcgccttc ccggatcccg gtgtcgacga cccgccccga
      241 cgccatcccg acccggtcca ggacggtctc gatgatcgcc gcacccgcgg ccaggagccg
      301 gatccgggcc tcgcgcatcc cgtaggtcgc ggcgagcatc tcggccgggg tcgcggtcac
      361 gatgtcgcgg acgatcccga gcgctacccg gtcgagttcg tctccccggt ccggactgcc
      421 gacccgccgc agcagcttga gcaggttgga agctgttccg ccgacgaaga cgacctcgac
      481 tggcggccgg gtcggggtcc agtcgaacgc ctcgtcggcc gcgcggtaga gcgccgccac
      541 gtgctcgacc gaaggcggat cgcgatcgac gatccggcgg gtgagccggc cggcgccgac
      601 ctggacaccg gccgcccggg ccagcctgcc cggcccgatc tcgacgagct cgctcgatcc
      661 tccgccgatg tcgaccacga gcagatcgcc cttcacggct cgcccggccg tcagtccgac
      721 gagggtgagc atcgcctcct cctcgtgctc gaggacggcc agcggctcac ccgcgcgggc
      781 ggatacctcg gcgacgatcc tgagcgcgtc gctcaggcgt cggagtggct ccgtgcccat
      841 gacgatcggg tggagcgctc cgtgggcccg tgcccgctcg acgtacgacg cgaccgtcgc
      901 caccagcgtc gttcgcccgg ccgcgccgag ggcacccggt ccgtcgaccg cggcgcccag
      961 cccgaggaat gccgattcat cctcgaggac gaccagcccg gttcgctccg ggcgggcgat
     1021 gaggaggtgg acggacgtcg agccgaggtc gatcgcggcg ccgggtcgcc tgggcacgcg
     1081 ggcacgcggc gctcaggcca gcgcgtgcgt tcggcgagcg atcacgcctg accgtcggct
     1141 gacgggccag cggcaggtcg cggtccacgc cgggccgtgg ccttgtcagc cggcttggcc
     1201 gcggccgcgc gggccttcgt ggtcgatgcc ttcttgcggg tcgccgagac cgcgcgctct
     1261 gcggtcgcta cggccggggt cgcggtggcg ggagtcgtgg cggcgggagc cggagcggca
     1321 tcggcggccg tggcggcggc cgatccggtg gccgacgccc ggttcgcagc cttgggcggc
     1381 gccgccgctc gcgtccggcg ggcgccggag ccgcgcggtc gaccgggtcc tcggccggtg
     1441 cgctgcgttc cctgagcctg gagcctgtga accgcatcga cgagcgccgc gagctcggcg
     1501 atctcgtcgc ggacctcgga gagccgcgcg gtgcgcttct cccgactttt cttcgtcgcc
     1561 ttttcgagcc gaccggcccg ccatgtctcc tccgagcgcg cagccgcgag ggcgaactcc
     1621 agccactcga cgtggcgagc gagggccgcg gcgggggtga tggcgcccgc ccgccgaccc
     1681 tggttgcgcc cattgctcga cggtgcggcg gctgcggtcg agccctcccc ggcgtcgctc
     1741 gtgcgtgcgt tcatgaatgt ctctccttct tggaccccgg cgtcattctc gcccatcacg
     1801 gggctccccg tcgcggttcc gcatcgtttc gcgttcggcg aggccggccg gaagcgaccg
     1861 cgaccgagcc agttggcagc tcgttgaccc gttgcaaacc aaacgaaaac gtcggcccgc
     1921 cacggttgag gccgggacct gcgacgcagc tcggacctgc gctggtcccg ctcctcctcg
     1981 gccggtgggt catggaccca tcggccgatc gccaccgacg ccatggaccc gagcacggtc
     2041 gccggggcga cccgaacgat gagcgaggtc ctccgatcga tcccgttgtc gcgacttcga
     2101 cgaaccctct ggccggtccg ccggcgccgg gggcctcgca gacctggttg atccttgccg
     2161 gtcaccatgc gggcgtccca tcgccggggc ccgatgtcga catcgtcgtc gcggcggacg
     2221 gaccggcgtt tgtcggcttc ctccatgagc tcaaggcgtg cctggccctg ttgacctgcc
     2281 cgccgggcgg cccgaccgag atcctcgccg ccgtgaccga tcgacgccag cggcccgccc
     2341 tccggatcat cctcctcaac gaccctgccg atgtgaccgg ccggctcgag gcgctcgccc
     2401 tcggcttcga cgacgccctc ccggcgtcca tcgaccccga ggaactgacc ggccgggccc
     2461 tcctgctggc gaacgggaat cggggtgtcg ccggccacga gcggcagatc ccggtcgctc
     2521 cgggcgtcat cctcgacctg gtcggccggc gcgtccggcg cgggggcgtc gatgtccatc
     2581 tccgaccgaa ggagttcgcg ctgctctcgg tcctggcgtc cgatccgggc cgggtcttca
     2641 gccggaccga gctcgtggac cgcgcgtggg gtccggccta tgacggtggc tcgcgaacca
     2701 tcgacgtcca catccgctgg ctgcgggcca agatcgaacg agatcccgct cgaccgagcc
     2761 acgtggtcac cgtccgcggc acgggctacc gcctcgatcc attcgtggac tgaccactgc
     2821 gcccacgatc gggatcgggc cttggccccc gtgccgttca tcaatcgtta acgcgcggca
     2881 aaggtccggt tcatccagaa gctggacggt tcaggcgttg ccacgcattg ccacgcgccc
     2941 gagctccgtc ggagtcgggg ccacaacatc cgtcggtcgt gcgccggcgg cagaggagga
     3001 cacgttgatc gaacgaagcc ggcgccttgg cgccgtgatg atcatggccg gcctgctggc
     3061 cacggcctgc agctcaggcg ccacgccgag cccatcgtcg agcggcggcg ctgctcccag
     3121 caccggagcc tcggcggcgg cccccggatc gcaggcggcg gctctgtcgg gctccgtcaa
     3181 tatcgacgga tcgagcacgg tctatccgat caccgaggcc gtggcggagg agttccagaa
     3241 ggccaacaag ggcgtcaagg tcacggtcaa cttcgccggc accggcggcg gcttcaagaa
     3301 gttctgcaac ggcgagaccg acatgaacga cgcctcgcgg ccgatcaagg ccgatgacgc
     3361 cggtgaaggc gccggctgca aggcgaaggg catcgaatgg ctcgagctcg gcatcgccac
     3421 ggacgccctg tcggttgtgg tcagcaagga caactccttc gtcacctgcc tcacgacggc
     3481 ccagctcaag acgatgtggg accagggcag cacggccaag tcgtggaagg acatcgaccc
     3541 gtccttcccg gatgagcccc tcaagctgtt cgggcccggt ccggactccg gcacgttcga
     3601 ctacttcacc gaggtcatca acggcaaggc caagcagagc cggtcggact acacgccctc
     3661 cgaggacgac aacgcgctcg tcaccggcgt cgccggcgac aaggacgcgc tcggctactt
     3721 cgggttcgcc tacctcgagg agaacctcga caagatcaag gcggtcgcga tcgacagcgg
     3781 cggcgggtgc atcgagccct cggccgacaa cgtcaacggc ggcaagtaca agcccctcgc
     3841 ccgcccgctc ttcatctacc cggcggtcag cgccctcaag cgccccgagt tcgccgcctt
     3901 cgtccagtac tacctcgcca acgtcaacac gtatgtcgac gagaccggct acatcgaggc
     3961 cctcccggct gtcctcgaca agtccaaggc agacctggcg gccgcactca agtagcgttg
     4021 cgatccagtc tcgacgcacc ggcccggagc ttggatcggg ccggtgcgtc tcctcgattc
     4081 cgggtagggt gcacgtgaaa tgaccgccgc gagccgcccg ctcaccagac gtcgaccgat
     4141 gtcggagcgg atcatcgaga cgctcctcct ggtcgccgcc agcgtcggcg tgctgaccac
     4201 gatcgggatc gtcatcgtgc tgatgatcga ggcggtccag ttcttcggcc tggtcaaccc
     4261 gatcgacttc ctgactggca cggtctggtc cgcctcgatc ctgccgttcc ggttcggggt
     4321 cattccgctc gtcgtctcga cgctcgaggt cgccctgctc gccctgatcg tggccgtccc
     4381 gctcggtctg ctggcggccg tgtacctcgc ggagtacgcg accctccgca cccgcaacat
     4441 cgtcaaaccc atcctcgaga cgatcgccgg catcccgacg atcgtcctcg gcttcttcgc
     4501 cttgaacttc atcacgccgg atctcctgaa gccgctcctc ccgaccatcg gcacgttcaa
     4561 catcctgtcg gccgggctcg tggtgggcct gctgatcacg cccctcatcg cgtcgctatc
     4621 ggaggatgcc ctgcgggccg ttccgcgcgg actccgcgag ggcgcgttcg ccatgggggc
     4681 gacccggttc gaggtcgtcc ggcgggttgt ctttccggcg gccctgtcgg gcgtgatggc
     4741 ctcgatcatc ctggcgatgt cgcgggccgt cggcgagacg atggccgtcg tgctggccgc
     4801 cggcttcaac ccgaagctca cgatcgatcc gcgcgaccag ggcgggacga tggcggcgtt
     4861 catcgcccag atcacgagcg gagacacgcc ccaggactcg atccagttca agtcgctttt
     4921 cgccgtcgcg ctcgtcctgt tcgcgatcac cctgctcctg aacctcatca gcaaccggat
     4981 cgtcgcccgc taccggaaca agtacgcatg agcgcactga cgactcccgc gacgggatcc
     5041 gtgccgcgct tcgatccgcg catcgccaag cgcaggatga ccgggacgat cttcctcggg
     5101 gcgtgcatcg ccgcgatcgg catcctcctg atcgcgctgg tcgcgctcct ggtggacgtc
     5161 gtctcgaagg gcgtgccgtg gctcgacatc gacttcctga ccggcgtgcc gtcgcgccgg
     5221 gccgcgacag ccggcatcct gcccgccctg gtcggctcgc tggagatctc cctgctgacc
     5281 ggcctgatct cggtccccgt cggcgttggc gccgcgatct acctccagga gtacgcccgc
     5341 gacacgttcc tcacccggac cttgcgcacg aacatcgcga acctggccgg cgtgccgtcg
     5401 atcatctacg ggatcttcgg gttggcgatc ttcgtccgcg cgctgagctt gggacggtcg
     5461 cccctggcgg cggcgctcac gctcgccctg ttgatcatgc cgatcgtcat catcgccacg
     5521 atcgaggcgc tccgggcggt cccggactcg caacgggagg gcgcctacgc cctcggggcg
     5581 acccgctggc agacggttcg cggatccgtc ctgcccgccg cagcgccggg catcgtcacc
     5641 ggggtcatcc tggcgatcgc ccgggccatc ggcgaggcgg caccgctcat cgtcatcggc
     5701 gcttccgcgt tcctggtctt cctgccgacg ccgctccagc agggcttcag cgtgctgccg
     5761 atccaggtct tccagtggtc gagccggccc caggccgact tccagggcat cgccgccgcc
     5821 acgatcctcg tgatgctgtt cctgacggcc gtcctgaacg tcgtggccat cgtcgtccgc
     5881 aaccggctgg cccggaacat ccagtggtag aggagtcgtc cgtgaacgcg tccatgaccg
     5941 aggcgccgcc gcatgccgcg cagccggcgt cggcggcgat cgccagcgac ggtccgccac
     6001 gcgtccgcat cgccctcgac gga
//
LOCUS       DATAIW010000385         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_174503, whole genome shotgun sequence.
ACCESSION   DATAIW010000385 DATAIW010000000
VERSION     DATAIW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174503"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1034)
                     /locus_tag="VHM48_11500"
     CDS             complement(69..1034)
                     /locus_tag="VHM48_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2756084.1"
                     /translation="MASRSSRLDWLIFLALGFFWGSSYLFIKIGVDNGLQPFTLIMFR
                     LLIGLALLATVVGFAREPLPRDPRMYGHLFVMGVINIAIPFSLITFAEQTVDSSLASV
                     INSGVPLFVIVIAAIFLKGETVTLNRVAGLVVGFIGVAILVGLDVTNLNSADTVGELA
                     LVGATISYAAGNVYAKAHVHGLRPMIPAVLQVFFGLLVTGTLAFLTEHPLAVVPAPDA
                     ILAVVWLGLLGSGLAYLCYFRILQHWGATRTSMVAYLLPVYGLALGAIVLSEPITRST
                     LVGAALVIGGVALVNARFGTRPLFTRGASSTATPDGAARVGSSRS"
     gene            1269..>2200
                     /gene="argH"
                     /locus_tag="VHM48_11505"
     CDS             1269..>2200
                     /gene="argH"
                     /locus_tag="VHM48_11505"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2756085.1"
                     /translation="MRLWGGRFATDSDEQVAAFGRSIEIDAEMALDDLDGSVAHVRGL
                     GRAGILTEAEVAVLVDGLADLRRDVENGKLRWDPALEDVHLNLEAALTERLGPVAGKL
                     HTGRSRNDQVATDLRLWTRRAIDRLDRALLGFEDALVGLAERDGDAVMPGSTHTQPAQ
                     PVLFGHHLLAYVEMAERDRGRLADARRRLNLSPLGAGALAGAGYALDREATAADLGFD
                     GVTANSLDAVGDRDFVVEILAAVALGMVHLSRLAEEIAWWSNPRFGFVRVADAYSTGS
                     SMMPNKRNPDPAELIRGRTAAAIGAVTGVLALLKG"
BASE COUNT          356 a          751 c          789 g          304 t
ORIGIN      
        1 tcccggtgcc ggatctcccg cgcgagcatc cgcatcgcct gcgccggcac ctgtgcccgc
       61 gagcgggtct acgaccggct cgacccgacc cgggctgcgc cgtctggcgt ggccgtcgac
      121 gatgcaccac gcgtgaacag cggccgtgtg ccgaaccgcg cgttcacgag ggcgacgccg
      181 ccgatgacga gcgccgcccc gacgagcgtg ctccgcgtga tcggctcgct caggacgatt
      241 gccccgaggg cgagaccgta gaccggcagc aggtacgcga ccatcgaggt tcgagtcgca
      301 ccccagtgct ggaggatccg gaagtagcac aggtacgcga ggccggagcc gaggaggccg
      361 agccagacga ccgcgaggat ggcgtcgggt gcgggaacga cggccagcgg atgctcggtc
      421 aggaacgcga gcgtgcccgt cacgagcagt ccgaagaaga cctggagcac cgccgggatc
      481 atcggccgga ggccgtggac atgggccttg gcgtagacgt tgccggcggc gtacgagatc
      541 gtcgccccga ccagggccag ctcaccgacc gtgtcggccg agttgaggtt cgtcacatcg
      601 agcccgacca ggatcgccac gccgatgaac ccgaccacga ggccggcgac ccggttgagc
      661 gtcacggtct cgcccttgag gaagatcgcg gcgatgacga tgacgaagag cggcacgccg
      721 ctgttgatca ccgatgcgag cgatgagtcg accgtctgct cggcgaaggt gatgaggctg
      781 aagggaatgg cgatgttgat cacgcccatc acgaagagat ggccgtacat ccgcggatca
      841 cgcggcaacg gctcacgggc gaagccgacg accgtcgcga gcagcgcgag accgatgagg
      901 agccggaaca tgatcagcgt gaacggctgg aggccgttgt cgacgccgat cttgatgaac
      961 aggtagctgc tgccccagaa gaagccgagg gcgaggaaga tcagccagtc gagacgcgag
     1021 ctgcgggatg ccatgggacc tcatgatcgc tgattgcttg gcctagcgca atgggccaac
     1081 tcgagctggg cgccgggcag tcaaccgata gaacacctgg gccggcgtcg ccgttgcagg
     1141 acggtggcag cggtcctcgc gatcctcgca tcggatcctg ccggatggcg gcatgattgc
     1201 ggtcgcgggg tcgggccgat cggcccggtc ggcaggctgc ggcggtgggc gagcgatgag
     1261 gaggtcggat gcggctctgg ggcggacggt tcgcgaccga cagcgacgag caggtcgcgg
     1321 ccttcgggcg ttcgatcgag atcgacgccg agatggccct cgacgacctc gacggctcgg
     1381 tggcccatgt ccgtggtctt ggccgggcag gaatcctgac cgaggcggag gtggcggtcc
     1441 tcgtcgacgg cctcgccgac ctgcggcgtg acgtcgagaa cggcaagctt cgctgggatc
     1501 ccgcgctcga agacgtccat ctcaacctcg aggccgccct gactgaacgc ctgggcccgg
     1561 tcgccggcaa gctccacacg gggcgctcgc ggaacgacca ggtcgcgacc gacctgcgcc
     1621 tctggacccg ccgcgccatc gatcgcctcg accgcgccct gctgggcttc gaggacgccc
     1681 tcgtcggtct cgccgagcgc gacggcgacg cggtcatgcc gggctcgacc cacacccagc
     1741 cggcccaacc ggtcctcttc ggtcaccacc tgctcgccta cgtcgagatg gccgagcgcg
     1801 atcgcggccg cctcgccgac gcccgccggc gcctcaacct cagcccgctc ggtgcgggcg
     1861 ccctggccgg cgccggctac gccctcgatc gcgaggcgac cgccgcggat ctcgggttcg
     1921 acggggtgac ggccaactcc ctcgacgcgg tcggcgatcg cgacttcgtc gtcgagatcc
     1981 tggccgcggt ggcgctcggg atggtccacc tcagccgtct cgccgaggag atcgcgtggt
     2041 ggtcgaatcc gcgcttcggc ttcgtccgcg tcgccgacgc gtactcgacc ggcagctcga
     2101 tgatgccgaa caagcgcaac cccgatccgg ccgagttgat ccggggccgg accgcggccg
     2161 cgatcggcgc cgtcaccggc gtcctggctc tcctcaaggg
//
LOCUS       DATAIW010000386         6687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_174647, whole genome shotgun sequence.
ACCESSION   DATAIW010000386 DATAIW010000000
VERSION     DATAIW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174647"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VHM48_11510"
     CDS             complement(<1..1514)
                     /locus_tag="VHM48_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756086.1"
                     /translation="MSRFNAWSLLAVFGIGVFLAGLELMITATALPSILLDLALTDQL
                     GKPAFSELRHASWIVNGYLLAYVVAMPLAGRLADVWGARRLFLAALVIFVIGSLVAGR
                     GQTLDELIVGRIIQGIGGGVLVPVGTAAASHLFEGHDRPRALGIIGGLTFLGMAAGPF
                     LGAAILGGFSVSGGLDRLGVADPSALRDALTPAWRWVFYVNVPIGLAALIIAWAASHG
                     WETPRRRAGPDVVGAVVWSVALAAALGATTLIGVTDIGGPDPLQVGVALVVLAVVATL
                     VTVVRGLRRPDPFLDPRLFRSMAFSSAALVSLLTGFAFATAIIGAAVFVDRVLYGGPD
                     QQRLALGALAGATALGALVSGFAVRAFSLRIVTLAGLLLSAATLWLMAGWTPAVPIGT
                     VALVLGAFGFGFGLTVTPRSTAAVESAGRASFGMASAIVTVARMIGMAIGVAILTAYG
                     STTIDRLYDRLFSAANPDGWKDVIPAELRDRPLRDGLVVDALEAWAAREASGILV"
     gene            1859..2227
                     /locus_tag="VHM48_11515"
     CDS             1859..2227
                     /locus_tag="VHM48_11515"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2756087.1"
                     /translation="MTLSPSPARVRSTIANAVLQPDELRELRLVHKALADVNRLRIVR
                     TLAVAEATVAELVAQVRLSQPLVSWHVGRLRAAGLVATRRIGRETICRLRPEAFVAFA
                     ARQADILGIAAAVDRERAAS"
     gene            2224..2940
                     /locus_tag="VHM48_11520"
     CDS             2224..2940
                     /locus_tag="VHM48_11520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2756088.1"
                     /translation="MIGSVVSPELRARVRGLALPVALAFGKMGLTPNALTVLGFGISV
                     VAAIAATQQAWILAGLLVIFGGVFDLFDGALARATNRVSRLGAFLDSVFDRAGEAVLY
                     LGIAIGCLDLNYHPGAILAATAMGASFMVSYTRAKSESLGFTPGTGMANVGVAPREVR
                     LILLTIGLLITGVLGGLGSYDDAMFGLTWDAGKPELVAALGLITILATITTIQRIVHV
                     TRQTPPESIGAATPGGDQHP"
     gene            2937..4172
                     /locus_tag="VHM48_11525"
     CDS             2937..4172
                     /locus_tag="VHM48_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX2756089.1"
                     /translation="MSTSNGANGASANGHKPGKKIRVAIVGVGNCASSLVQGRYYYEN
                     AKVEDTVPGLMHVELGGYHVRDIEFVAAFDIDKNKVGKDLSEAIYQKPNNTYVFQQVP
                     RLDVKVERGMTHDGLGKYLSQIIEKAPGPTADVVGILKERQVDVVVSYLPVGSEEATK
                     WYVEQALRAGVGFVNAIPVFIGREPYWQRRFAEEGLPVIGDDIKSQVGATISHRVLTR
                     LFMDRGVRLDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLDYEIDPDNVHV
                     GPSDHVPWLEDRKWCYIRMEGTTYGDVPLNAELKLEVWDSPNSAGVITDAIRCLKLGL
                     DRGLKGTLVAPSSYFMKSPPIQIHDDISHNRLEAFIRGEDNETLVGTENLTKAQLKKL
                     ASTAPVASAAVKKAAAAAR"
     gene            4169..5356
                     /locus_tag="VHM48_11530"
     CDS             4169..5356
                     /locus_tag="VHM48_11530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756090.1"
                     /translation="MTGRKGLEGPHRRREQGPQADGVAGLRFGLRRVTGRFGGRTVER
                     LAVTAYAAAVWLVAHVPAGLARWVIGTASQAGYLLWPTKRAWSNANFGRVAGLPPDDR
                     RVRRLALAAYREYATYLVEVMRLESLSVAEAVSRVVQTDLDQIEAAWKSAPGGGLIFV
                     LGHVGNNEAVAAGVADRGWPINVVADDSSFPEMFERFRRLREAYGVHVIPWRNLREIY
                     TVLKNREMLALLIDWGYRADGVPVRMFEAWTTLPAGPATLAAKTKSLILPVAIRRGDD
                     EMFHVSFSPTIAVASASDADVARATQEIATALEATIAAAPEQWYSFKPMWPPTDEEAA
                     GLAARAARMLAGLPEPRPGSPAADMPAQAGEAPAQAGEAPAGEAPAADVPGGEPATGF
                     APS"
     gene            5353..6375
                     /locus_tag="VHM48_11535"
     CDS             5353..6375
                     /locus_tag="VHM48_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2756091.1"
                     /translation="MTDALPTPAAEGSAAARDRGTPVQRVRVLGLRGLSWLACRLPER
                     LAVALAEVVGWLWYKLAPARAAQARRNLARVVAALDARGQGSARVRAAAHDARALERL
                     VVAAFRHDTRYYLEILRVPSLRPEVFERSVVVETPDAVEQAFVGGPVIFVSPHLGPIE
                     LPALYLANRSGRTFVAPMETVDDPPLQGWFEASRGRLGVRIVGLRAARRALLAAVRAG
                     EPIGIVADRDIAGGGLDVPLFGAPAPLPIGPALIAIETGAPLFAVGVRRLADRRVAGR
                     LLAVPVAAEGTRRERIAATLAAMAAAFESLIADAPEQWSAVFFPIWPDLEADAAPTEA
                     ASTTAR"
     gene            6372..>6687
                     /locus_tag="VHM48_11540"
     CDS             6372..>6687
                     /locus_tag="VHM48_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756092.1"
                     /translation="MTNRLGRADLHIHTLASDGIDSIATILDHIERSTDLDVVAITDH
                     ERIDAALAARTMAHELGQRAEIVVGEEISTLGGHLLALFIEERVRPLRGLRTTIAEIH
                     EQA"
BASE COUNT         1042 a         2468 c         2295 g          882 t
ORIGIN      
        1 acgaggatcc ccgacgcctc cctcgccgcc caggcttcaa gagcgtcgac gacgagcccg
       61 tcgcggagcg gccggtcgcg gagctcggcc gggatcacgt ccttccaacc gtccggattt
      121 gccgccgaga acaggcgatc gtagaggcgg tcgatcgtcg tcgatccgta ggccgtcagg
      181 atcgcgacgc cgatggccat cccgatcatc cgggccaccg tgacgatcgc gctggccatc
      241 ccgaacgacg cgcgcccggc gctttcgacc gcggcggtgc tgcgcggcgt cacggtcagg
      301 ccgaacccga acccgaacgc gccgagaacg agggcgaccg tcccgatcgg caccgccggg
      361 gtccagcccg ccatcagcca gagagtcgcc gcgctgagca gcaggccggc gagcgtcacg
      421 atccggagcg agaacgcgcg gaccgcgaac cccgacacga gcgccccgag cgccgtcgca
      481 cccgccagcg caccaagggc gagccgctgc tgatccgggc cgccgtacag gacgcggtcg
      541 acgaacacgg cggcgccgat gatcgcggtc gcgaacgcga agcccgtcag cagcgacacc
      601 agcgcggccg acgagaacgc catgctccgg aagagacgcg gatcgaggaa cgggtcagga
      661 cgacgcaggc cgcgaacgac ggtgacgagg gtggcgacga ccgcgaggac cacgagggcg
      721 acgccgacct ggagcgggtc cggcccgccg atgtcggtga cgccgatcag ggtggtcgcg
      781 ccgagggcgg cggccagggc gacgctccag acgaccgccc cgaccacgtc cgggccggct
      841 cgccggcgcg gcgtctccca gccgtgcgag gcggcccagg cgatgatcag ggccgcgagc
      901 ccgatcggga cgttgacgta gaacacccag cgccaggccg gggtcagggc atcgcggagt
      961 gcgctcgggt cggccacgcc gaggcgatcg aggccaccgg acacgctgaa cccgccaagg
     1021 atcgccgcgc ccaggaatgg ccctgccgcc atcccgagga acgtcagccc gccgatgatc
     1081 ccgagcgccc gcggccggtc gtggccctcg aacaggtgcg atgccgccgc ggtcccgacc
     1141 gggacgagga cgccgccgcc gatcccctgg atgatccggc cgacgatcag ctcgtcgagc
     1201 gtctggccgc ggcccgccac cagcgacccg atgacgaaga tcacgagcgc cgcgaggaac
     1261 agccgccggg cgccccagac gtcggccagc cgacccgcca gcggcatcgc cacgacatac
     1321 gccaggaggt agccgttgac gatccacgac gcgtggcgga gctcggagaa ggcgggcttg
     1381 ccgagctggt cggtcagcgc gaggtcgagc aggatcgacg gcaacgcggt cgccgtgatc
     1441 atcagctcga ggcccgcgag gaagaccccg atcccgaaga cggcgaggag gctccaggcg
     1501 ttgaaccggc tcaccctcgc agggtacagg cgcgggccgt ggtccggccg gccgtgcccc
     1561 tgccgatatc gatgccattg ctctcggccg gccagatcgg ccctcaggag acgcaattct
     1621 ggctcgtagc ggcgcgatcg accgcgccgt gcgtggcatc gatggaatcg agccgtaatg
     1681 agcacgccaa gcccggagat atcgatgggg tttcatgttg cggtccagag gcgcgccgtc
     1741 ggcccgacac cgggcaagag cagggttgac agcctgcacc ggacctcgtt aggatcaacc
     1801 cccattgata taaaccgccg ttgatccgac ggccggcatc accgcccgca gtggagcctt
     1861 gaccctgagc ccgtctcccg cccgcgttcg cagcacgatc gccaacgccg tcctccagcc
     1921 ggacgagttg cgcgagctgc ggctcgtcca caaggcgctc gccgacgtca accgcctccg
     1981 gatcgtccgg accctcgccg tcgcggaggc gacggtcgcc gagctcgtcg cccaggtccg
     2041 cctgtcgcag ccgctcgtct cgtggcatgt cgggcgcctg cgggccgccg gtctcgtcgc
     2101 gacgcgcagg atcggccggg agaccatctg ccgtctccgg cccgaggcat tcgtcgcctt
     2161 cgcggcacgc caggcggaca ttctcggcat cgccgcggcg gtcgatcgcg agcgggcggc
     2221 ctcgtgatcg gctcggttgt cagcccggag ctccgggcgc gtgtccgcgg cctcgccctc
     2281 cccgtcgccc tcgccttcgg gaagatggga ctgacaccca atgccctgac agtcctcggc
     2341 ttcgggatct ccgtcgtcgc cgccatcgcc gccacccagc aggcgtggat cctggccggc
     2401 ctcctcgtca tcttcggcgg cgtcttcgac ctcttcgacg gcgccctcgc ccgcgccacc
     2461 aaccgggtga gtcggctcgg cgccttcctc gactcggtct tcgaccgggc cggcgaggcg
     2521 gtcctctatc tcggcatcgc gatcggatgc ctcgacctca actaccaccc cggggcgatc
     2581 ctggcggcga ctgcgatggg cgcgtcgttc atggtcagct acacacgggc aaagtcggag
     2641 agcctcggct tcacgccggg gaccggcatg gccaacgtcg gtgtcgcccc acgcgaagtc
     2701 cgtctcatcc tgctgacgat cgggctcctg atcaccggcg tcctcggcgg cctcggcagc
     2761 tacgacgacg cgatgttcgg gctgacctgg gacgccggca aacccgagct ggtggccgcc
     2821 ctcggcctga tcaccatcct cgccacgatc accacgatcc agcgaatcgt ccacgtcacc
     2881 cgccagaccc cgccggaatc gatcggcgca gccaccccag gaggagacca gcacccgtga
     2941 gcacttcgaa cggagccaac ggcgcgagcg ccaacggcca caagcccggc aagaagatcc
     3001 gcgtcgcgat cgtcggcgtc ggcaactgcg cgagcagcct cgtccagggc cgctactact
     3061 acgagaacgc caaggtcgag gacactgtcc cgggcctgat gcacgtcgag ctcggcggct
     3121 atcacgtccg cgatatcgag ttcgtcgccg ccttcgacat cgacaagaac aaggtcggca
     3181 aggacttgtc cgaggcgatc taccagaagc cgaacaacac ctacgtcttc cagcaggtcc
     3241 ccaggctcga cgtcaaggtc gaacgcggga tgacccacga cggcctcggc aagtacctca
     3301 gccagatcat cgagaaggcg cccggcccga cggccgacgt cgtcggcatc ctcaaggagc
     3361 gccaggtcga tgtcgtcgtc tcgtatctgc cggtcggcag cgaggaagcg acgaagtggt
     3421 acgtcgagca ggcccttcgg gccggcgtcg gattcgtcaa cgcgatcccg gtgttcatcg
     3481 gccgcgagcc ctactggcag cgccgcttcg ccgaggaagg gctgccggtc atcggtgacg
     3541 acatcaagag ccaggtcggg gcgacgatca gccaccgcgt cctgacccgc ctgttcatgg
     3601 accgcggcgt ccggctcgac cggacctacc agctgaactt cggcggcaac accgacttcc
     3661 tgaacatgct cgagcgcgag cgactcgagt ccaagaagat cagcaagacc aacgcggtca
     3721 catcgatgct cgactacgag atcgacccgg acaacgtcca cgtcggcccg agcgaccacg
     3781 tgccgtggct cgaggaccgc aagtggtgct acatccggat ggagggcacg acgtacggtg
     3841 acgtcccgct caacgccgag ctcaagctag aggtctggga cagccccaac agcgccggcg
     3901 tcatcaccga cgcgatccgc tgcctcaagc tcggcctcga tcgcgggctg aagggcaccc
     3961 tcgtcgcccc gtcgagctac ttcatgaaga gcccgccgat ccagatccac gacgacatct
     4021 cgcacaaccg cctcgaggcg ttcatccgcg gcgaggacaa cgagacgctc gtcgggaccg
     4081 agaacctgac gaaggcccag ctcaagaagc tggcatcgac ggcgcccgtc gcctcggccg
     4141 ccgtcaagaa ggcggcggcc gcagcccggt gacgggccgc aagggactcg aagggcccca
     4201 tcgccggcgc gagcagggtc ctcaggccga tggcgtcgcg gggctccgct tcggacttcg
     4261 gagggtcaca ggacggttcg gcggccgaac agtcgagcgg ctggccgtca ccgcctacgc
     4321 ggcggcggtc tggctcgtcg cccacgtgcc ggccgggctc gcccgctggg tgatcggcac
     4381 cgcgagccag gcgggctacc tgctctggcc gacgaagcgg gcatggtcga acgccaactt
     4441 cgggcgggtc gcggggttgc cgccggacga tcgtcgggtg cgccggctgg cgctcgcggc
     4501 ctaccgcgaa tatgcgacct atctcgtgga ggtcatgcgg ctcgagtcgc tgtccgtcgc
     4561 ggaggccgtc agccgtgtcg tccagaccga tctcgaccag atcgaggcgg cctggaagtc
     4621 ggcgcccggc ggtggcctga tcttcgtcct gggccacgtc ggcaacaacg aggcggtcgc
     4681 ggccggcgtc gccgaccgcg gctggccgat caacgtcgtg gctgacgact cgagctttcc
     4741 cgagatgttc gagcgcttcc gccgcctgcg cgaggcgtac ggcgtccacg tcatcccttg
     4801 gcggaacctc cgcgagatct acacggtcct caagaaccgc gagatgctcg ccctgctgat
     4861 cgactggggc taccgcgccg acggcgtgcc ggtccggatg ttcgaggcgt ggacgaccct
     4921 gcccgccggc cccgcgaccc tcgctgccaa gacgaagtcg ctgatcctgc cggtcgcgat
     4981 tcgacgcggc gacgacgaga tgttccacgt ctcgttctcg ccgacgatcg cggtcgcgtc
     5041 cgcgtccgac gccgacgtcg cccgggcgac ccaggagatc gcgaccgccc tcgaggcgac
     5101 gatcgccgcc gcgccggagc agtggtacag cttcaagccg atgtggccgc cgacggacga
     5161 ggaggccgcc gggctcgccg cccgcgccgc ccggatgctc gccggcctgc ccgagccgcg
     5221 gcccggaagc ccggccgccg acatgccggc acaagcgggc gaagcgccgg cccaagcggg
     5281 cgaagcgccg gcgggcgaag cgccagcggc cgacgtcccc ggcggcgaac cggcgaccgg
     5341 gttcgcccca tcgtgaccga cgcgctcccg acaccggccg ccgaggggtc cgcggccgct
     5401 cgcgaccggg gcacgcccgt ccagcgggtg cgggtcctgg gtctgcgtgg cctgtcgtgg
     5461 ctcgcctgtc gactccccga gcggctcgcc gtcgccctcg ccgaggtcgt cggctggctc
     5521 tggtacaagc tcgccccggc ccgcgcggcg caggccaggc gaaacctcgc ccgggtcgtg
     5581 gccgccctcg acgcgcgcgg gcagggatcc gcgcgagtcc gggccgccgc ccacgacgcg
     5641 cgcgccctgg agcgcctcgt cgtggccgcg ttccgccacg acacgcgcta ctacctcgag
     5701 atcctccgcg tcccgagcct ccgtccggag gtcttcgagc ggagcgtcgt cgtcgagacg
     5761 cccgacgcgg tcgagcaggc attcgtcggt ggcccggtga tcttcgtgtc cccgcatctc
     5821 ggcccgatcg agctgcccgc gctgtacctc gcgaaccgct ccggccggac gttcgtcgcc
     5881 ccgatggaga ccgtcgacga cccacccctg cagggctggt tcgaggcatc ccgggggcgg
     5941 ttgggggtcc ggatcgtcgg cctgcgggcg gcgcgccggg cgctgcttgc cgcggtccgg
     6001 gcgggcgagc cgatcgggat cgtggccgac cgcgacatcg ccggtggcgg cctcgatgtg
     6061 cccctcttcg gagcgccggc accgctgccg atcggaccgg ccctgatcgc gatcgagacc
     6121 ggggcgccgc tcttcgccgt cggggtccgc cgcctcgcgg accgccgcgt ggcgggcagg
     6181 ctgctggccg tgccggttgc cgccgagggc acccgtcggg agcggatcgc cgcgacgctc
     6241 gccgcgatgg ccgcggcctt cgagtcgctg atcgccgatg caccagagca gtggtcggcg
     6301 gtgttcttcc cgatctggcc ggacctcgaa gcagacgccg cgccgaccga agcggcatcg
     6361 acgacagccc gatgaccaat cgcctcggtc gcgccgacct ccacatccac accctggcgt
     6421 cggacgggat cgactcgatc gcgacgatcc tcgatcacat cgaacgctcg accgatctcg
     6481 atgtcgtcgc gatcaccgac cacgagcgga tcgacgcggc cctcgccgcc cggacgatgg
     6541 cccacgagct cggccagcgg gccgagatcg tggtcgggga ggaaatctcg acgctcgggg
     6601 gccatctgct ggctctgttc atcgaggagc gggttcgacc gctccgcggc ctccggacga
     6661 cgatcgccga gatccacgag caggcgg
//
LOCUS       DATAIW010000387         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_55554, whole genome shotgun sequence.
ACCESSION   DATAIW010000387 DATAIW010000000
VERSION     DATAIW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55554"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VHM48_11545"
     CDS             complement(<1..1374)
                     /locus_tag="VHM48_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2756093.1"
                     /translation="MQPTTNAALSGPLTVAAGDGAAQSIDLQGGTRRFQRANREAAGG
                     ATGAGGPTRPRGVFDPDLLKAALPNAVRKLDPRQLIRNPVMFVVELTATLVTIIAIAN
                     LTGIQPAAPGTAGLGFQVQIGIWLWFTVLFATYAEAVAEARGRAQAATLRRTRSETTA
                     HRRRDDGSIEAVGSSELRRGDVIVVEAGETIASDGDVIEGLGFVNEAAITGESAPVLK
                     EPGTDIRSSVTGGTTLVSDGLVIVVTADPGETFLDRMISLVEGAKRQRTPNEIALAIL
                     LAGLTIIFLMATVTLRPFGAFAGTTVDTVALVALLVCLIPTTIGGLLSAIGIAGMDRV
                     ARFNVLAMSGRAVEASGDVDVILLDKTGTITYGNRLAASITAAPGVAEVDAVRAALLA
                     SIRDTTPEGRSIVEPARKRLAELGAAAGPDDAAGLAALDATIEEEIPFRAETRTSGVR
                     TTTGTTLK"
     gene            complement(1388..3097)
                     /gene="kdpA"
                     /locus_tag="VHM48_11550"
     CDS             complement(1388..3097)
                     /gene="kdpA"
                     /locus_tag="VHM48_11550"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX2756094.1"
                     /translation="MPVNDFLTLALLAVAILLITPFLGTYIHKVMEGERTFLSPVIRP
                     VERLVYRLSGIDETAEQGWRSYAASVLVFAFLAIVVGYVMLRLQDVLPLNPSGVAAQT
                     PDLALNTAVSFETNTNWQNYGGETGASHLIQATLLAVRNFTSAATGLAVAIALVRGLV
                     RRNSKTLGNYWVDLTRGVLYILLPISIVGAVILVWQGVPQTLDGPAAVTTLQGASQNI
                     ALGPIASQEWIKELGNNGGGFLNANSAHPFESPTGLTNFLEMLAILMAPFALTYTFGR
                     YAGNKRQGWTLFAAMATILVVGAVVAMHNEASPNTLLPGGIDQALGNMEGKETRFGAG
                     VGGLFAAVTTGTSTGAINAWHDSFAPLGGLVPLFNMELGEITPGGIGAGLYGMLVIGA
                     ILAVFIAGLMVGRTPEYLGKKIEAYEIKMAMIVALVLGASILGFTALATALPEGVAGP
                     LNHGPHGFSEILYAFSSQTGNNGSAFGGLTGNTPFYNVTGAIAMLIGRYGMIIPILAL
                     AGSLAAKRRVAPSLGTFPTTGPLWTVLVIGVVIIVGALTFFPALALGPIAEQFLQNAG
                     KAF"
     gene            complement(3101..3190)
                     /locus_tag="VHM48_11555"
     CDS             complement(3101..3190)
                     /locus_tag="VHM48_11555"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEX2756095.1"
                     /translation="MNALDILAGVAALFTFVYLLVALLRPEEF"
     gene            complement(3273..3695)
                     /locus_tag="VHM48_11560"
     CDS             complement(3273..3695)
                     /locus_tag="VHM48_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2756096.1"
                     /translation="MTESGGPIHRPAAGAPILIVEDDDPLREILARHLRARGHQIHEA
                     ASAEDAADALRGGLRPGLVLLDINLPGETGWSLLRGDALTIAGDPPVIVASATTVDPR
                     RLREHRVAGYLPKPFPLQTLLEAIERLTKPSESTEVPE"
BASE COUNT          559 a         1212 c         1414 g          561 t
ORIGIN      
        1 cttgagggtg gttccggtgg tcgtccggac cccgctggtg cgggtctcgg cccggaacgg
       61 gatctcttcc tcgatcgtcg cgtccagcgc ggccagcccg gccgcgtcgt cgggtcctgc
      121 ggcggcaccc agctcggcga ggcgcttccg ggcgggctcg acgatcgagc gcccctcggg
      181 ggtcgtgtcg cggatcgagg cgaggagggc ggcccggacc gcatcaacct cggcgacgcc
      241 gggcgccgcc gtgatcgagg ccgccaggcg gttgccgtag gtgatcgtcc ccgtcttgtc
      301 gaggaggatg acgtcgacgt cgcccgacgc ctcgacggcg cggccgctca tcgcgaggac
      361 gttgaaccgg gcaacccggt ccatcccggc gatgccgatc gcgctgagga gcccgccgat
      421 cgtcgtcggg atcaggcaca cgagcagggc gaccagggcg accgtgtcga ccgtcgtgcc
      481 ggcgaaggcg ccgaacggcc ggagggtgac cgtcgccatg aggaagatga tcgtcaggcc
      541 ggcgagcagg atcgcgaggg cgatctcgtt cggcgtgcgc tggcgcttgg cgccctcgac
      601 gagcgagatc atccggtcga ggaacgtctc gcccgggtcg gcggtgacga cgatgacgag
      661 gccgtcgctg acgagggtcg tgccgcccgt gaccgagctt cggatgtcgg tgcccggctc
      721 cttgaggacc ggtgccgatt cgccggtgat cgcggcctcg ttgacgaacc cgaggccctc
      781 gatgacgtcg ccgtcgctgg cgatcgtctc gccagcctcg acgacgatca cgtcgccgcg
      841 cctgagctcg gacgagccga cggcctcgat tgacccgtcg tcgcgccggc gatgggcggt
      901 cgtctccgag cgggtccggc ggagggtcgc tgcctgggcc cgaccgcggg cttccgcgac
      961 cgcctcggcg taggtggcga acaggaccgt gaaccacagc cagatgccga tctggacctg
     1021 gaagccgagg ccggccgttc ccggcgccgc gggctggatg ccggtcaggt tggcgatggc
     1081 gatgatcgtc acgagtgtcg ccgtgagctc cacgacgaac atcaccgggt tccggatcag
     1141 ctggcgcggg tcgagcttgc ggacggcgtt gggcagggct gccttgagga ggtccggatc
     1201 aaagacgccg cgcggtcggg tcggcccgcc cgcgcctgtg gcgccgccgg ccgcctcgcg
     1261 attggcgcgc tggaaccggc gggttccgcc ctggaggtcg atcgactggg cggcgccgtc
     1321 gccggcagcc accgtcagcg gaccgctcag ggcggcgttg gttgttggtt gcatcaactc
     1381 atccatctca gaaggccttg ccggcgttct ggaggaactg ctcggcgatc ggcccgaggg
     1441 ccagggcggg gaagaacgtc agggcgccga cgatgatcac gactccgatc acgaggacgg
     1501 tccacagcgg gccggtcgtc gggaacgtcc cgagggatgg tgccacgcgg cgtttggcgg
     1561 cgagcgagcc ggccagggcg aggatcggga tgatcatccc gtagcgcccg atcagcatcg
     1621 caatcgcacc cgtgacgttg tagaagggtg tgttgccggt caggccgccg aaggccgagc
     1681 cgttgttgcc ggtctggctc gagaaggcgt agaggatctc gctgaacccg tgcgggccat
     1741 ggttcaaggg gccggcgacg ccttccggca gcgccgtcgc gagggccgtg aagccgagga
     1801 tgctggcgcc gaggacgagg gcgacgatca tcgccatctt gatctcgtag gcctcgatct
     1861 tcttgccgag gtactccggc gtccgaccga ccatcagccc cgcgatgaac accgcgagga
     1921 tggcgccgat cacgagcatc ccgtacaggc cggcgccgat gccgccgggg gtgatctcgc
     1981 cgagctccat gttgaagagc gggacgaggc cgccgagcgg ggcgaagctg tcgtgccagg
     2041 cgttgatcgc acccgtgctc gtgccggtgg tgacggccgc gaacaggcct ccgacacctg
     2101 ccccgaagcg cgtctccttg ccttccatgt tgccgagcgc ctggtcgatc ccaccaggaa
     2161 ggagggtgtt cggcgaggcc tcgttgtgca tggcgaccac ggcgccgacg acgaggatcg
     2221 tcgccatcgc cgcgaagagc gtccagccct ggcgcttgtt gcctgcgtac cgtccgaacg
     2281 tgtacgtcag cgcgaacggc gccatcagga tcgcgagcat ctcgaggaag ttcgtcagcc
     2341 cggtcgggct ctcgaacgga tgggccgagt tcgcgttgag gaacccgccg ccgttgttgc
     2401 cgagctcctt gatccattcc tgggaggcga tcggtccgag ggcgatgttc tgcgaggcgc
     2461 cctggagcgt cgtcacggct gccggcccgt cgagggtctg gggcacgccc tgccacacga
     2521 ggatgacggc gccgacgatg gagatgggca gaaggatgta gaggacgccg cgggtcaggt
     2581 cgacccagta gttgccgagg gtcttcgagt tccgccgaac gaggccccgg acgagggcga
     2641 tcgccacggc gaggccggtc gccgcggagg tgaagttgcg gaccgccagg agcgtcgcct
     2701 ggatgaggtg gctcgccccg gtctcgccgc cgtagttctg ccagttcgtg ttcgtctcga
     2761 agctgaccgc ggtgttcagc gcgaggtccg gcgtctgggc ggccacgccg ctcgggttga
     2821 gcggcagcac gtcctgcagg cggagcatca cgtaacccac gacgatcgcc aggaacgcga
     2881 acacgaggac cgacgccgcg tagctgcgcc agccctgctc ggcggtctcg tcgatcccgg
     2941 acaagcggta gaccagtcgc tcgacgggcc ggatgaccgg gctcaggaag gttcgctcac
     3001 cttccatcac cttgtggatg tacgtcccga ggaacggcgt gatcagcagg atcgcgaccg
     3061 ccaggagggc aagggtgagg aagtcgttga cgggcatgga tcagaactcc tcggggcgga
     3121 ggagcgcgac gaggaggtac acgaacgtga agagcgccgc cacgccggcg aggatgtcga
     3181 gagcgttcat gaccggaccc ggtcgcagag gaccagatac ccggccagga tagccggggc
     3241 gaggatgagg aagacgaggg cgtgcaggtc ggtcattcgg gaacctcggt ggattcgctg
     3301 ggcttggtca gacgctcgat cgcctcgagc agcgtctgga gcgggaacgg cttggggaga
     3361 tatccggcga cacggtgctc gcgcaggcgg cgcgggtcga cggtcgtcgc cgaggcgacg
     3421 atcacgggcg gatccccggc gatggtgagc gcgtcgccgc ggaggaggct ccagcccgtc
     3481 tcgccgggca ggttgatgtc gagcaggacg agcccgggcc gcaggccgcc acgcagcgcg
     3541 tccgccgcgt cctcggccga tgccgcctcg tggatctggt gaccgcgcgc ccggaggtgg
     3601 cgggccagga tctcccggag cgggtcgtcg tcctcgacga tgaggatcgg cgcaccggcg
     3661 gccggtcggt ggatcgggcc gcccgattcc gtcactcctc gtcctcgtcg agggcctcga
     3721 ccgcgacgtc cgcgccacgc tcgtcg
//
LOCUS       DATAIW010000388         9534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_279433, whole genome shotgun sequence.
ACCESSION   DATAIW010000388 DATAIW010000000
VERSION     DATAIW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279433"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VHM48_11565"
     CDS             <1..788
                     /locus_tag="VHM48_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756097.1"
                     /translation="IPLSARARDDGAVQVITSSRASRPPLGAVVVGLVVGSVLLVGGL
                     LLAWVAFATPVLTGLTPPTARPGPAQLALGGVIWGFTLVAPPSFAIVGALRLGRVVRA
                     ISVKPRTRAMTRVAAAVGDQYSAATDVRLPDGRIVRDLVLGPFGLAVINELPPPRATR
                     HTGISWEIRRSDGRWVHFENPLERTARDGERVRRWIASTERDFVVKVYAAVVTPDPTI
                     SRTPGCAVLTPEQIPAWLASLPSARALTADRQVELVEAIRTIV"
     gene            complement(842..917)
                     /locus_tag="VHM48_11570"
     tRNA            complement(842..917)
                     /locus_tag="VHM48_11570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(882..884),aa:Ile,seq:tat)
     gene            complement(978..1280)
                     /locus_tag="VHM48_11575"
     CDS             complement(978..1280)
                     /locus_tag="VHM48_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756098.1"
                     /translation="MDEQHVALPKLYGAPAYARPTVVVPVAARPFDPDDLPIEAVQTD
                     EEREIAASLPAHMYAPGGSHGRPDRRFGRRDEEPGLHPRPLSLRALAGRILGQVDN"
     gene            complement(1446..1910)
                     /locus_tag="VHM48_11580"
     CDS             complement(1446..1910)
                     /locus_tag="VHM48_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756099.1"
                     /translation="MSVPAAAPEPGERQATPGTERPAPVPRREVRQRSWLEIRWRQFR
                     NAPRPVVRAVLSSLVVAIILGLGYLAYDVALRHGAVLPGGDLRTLYLALDIVTVLVVG
                     SVVTWLVVPQPRGSGLSTTRSPWSAALGFFAAVPVCYLVLVVVAQVLAPLIG"
     gene            complement(1907..3148)
                     /locus_tag="VHM48_11585"
     CDS             complement(1907..3148)
                     /locus_tag="VHM48_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2756100.1"
                     /translation="MVAPFLPDHEKLAAVRGALPALGAGIYLNTGSVGPMPDETAAAM
                     AELVDYELRIGRSHPDYWEGFLERMAEARASVAAVIGADVGEIALVHSTSQAMNAAVW
                     SIDLQPGDRIVTTQAEHPGGLGPVHVAAGRSGAELVFADVGHGGVDEATLAAFDAAIT
                     PRTRIVALSHVLWTTGARLPLREIATMAHERGARVIVDGAQAVGAIPVSVADLGVDFY
                     AVPGQKWLLGPEGTGALWCNPAILEASRPTNASWFTFERLSTAEAVPWRDARRFDDSG
                     HYRPGVTGLARSCGWLSMYVGLPWIHERGQALARAAADRLAGIAGVELLTPRDRMATL
                     VTFRIAGWTADETLAELNGRIFAIARTIPGLDAIRISVGFFTSTDEIERVAATVELLA
                     SHTPASLPPRPRLVILGQGEG"
     gene            3167..4051
                     /locus_tag="VHM48_11590"
     CDS             3167..4051
                     /locus_tag="VHM48_11590"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2756101.1"
                     /translation="MSSLPGRRLARTRAPGGLARPELLASTDWLADELGRPDVRILDV
                     RWRPDGSAHDVWAAGHIPGAVHVDWRADLTHPSEDGDTLLLAGPEQVARTLSVAGVGD
                     GATVVVYDDTVSLFAARTWWSLRVYGLESVRILDGGYPAWTEEGRPTSNATAPPPPAH
                     FTPRAQSRMRLTTTDVRALLSSPDVTLLDARAPAEYRGHEGNTKRLGHIPGAVNVPVG
                     AMTRAGSQHFKVGDELREHLFKSNIARGRRMVCYDGSGVAAAKLAFALTLLGHEDVAV
                     YDGGWAEWGNRLDLPVDR"
     gene            complement(4381..4950)
                     /locus_tag="VHM48_11595"
     CDS             complement(4381..4950)
                     /locus_tag="VHM48_11595"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756102.1"
                     /translation="MFESLLLGGEPSLVALTLYTDAFVIRGSIRSRQGRISDILNQAD
                     EEFLVLTDTVVDEYGARVTPVKSEFAQVNLGAVLFAVADTSVEARPDLRTPKVSEQAL
                     ISIPPFRVTGRIHLLPERDLREALSELTGRFIPVTDATYWSDSVGEARATAAMVAINH
                     HRAQILAPHREVDPWAGLDRSRQGGPTPT"
     gene            complement(5017..6717)
                     /locus_tag="VHM48_11600"
     CDS             complement(5017..6717)
                     /locus_tag="VHM48_11600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2756103.1"
                     /translation="MRRWSEVAERVGATTRTSEKTALLADYFADLSAAELPIAAVFLT
                     GRPFAEADQRAAGLGWSAIATTVTDLAGVPRAALGEAYDRSSDLGKAVEDVLTLAGHA
                     PDPSMSPTLPEVAAAYAGIEQASGPAAKSGILRDLFARSDPLTAKSIVKVLSGELRIG
                     LREGLVDAAIAKAFGRPLDAVKWAGMLTGDIGELAALARDDRLDEASLSLFHPIKFML
                     ASPAEDAAEIVKRLGVPVWVEDKYDGIRAQLHRDGDDVRLYSRDLHDISGQFPEIVDG
                     ARGLTWAGILDGEILAWRDGAVLPFIALQARLGRKSPSAAIRAEVPVIYVAFDVLGLG
                     RGGGDAPIEALLREPLEVRRRLLEGLALPLAGGGGVFVLSRLVSVTSEGALEDAFLGA
                     RERRNEGLMVKDPRSIYSPGRRGLGWLKMKKALATIDCVVVGVEFGHGKRHGVLSDYT
                     FAIRDTDNDRLVTIGKAYSGLTDAEIAEMTAWFEAHTIQRFGRYRQVEPTVVVEVAFD
                     VIVRSKRHQSGYSLRFPRIANLRPDKPPDEIDTLESVRALYEGLQPGAEQLVTAGARK
                     "
     gene            6745..7389
                     /locus_tag="VHM48_11605"
     CDS             6745..7389
                     /locus_tag="VHM48_11605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2756104.1"
                     /translation="MSPRTWFPETLTGEMVVLRRHAPANLRAFLRWYADPEVVRLTRY
                     QDSPMRPDEIERFFAARALGSESLAMAVHLRDGDRLIGTCALSQLDADNGSALFHITI
                     GEKDAWGQGHGTEATRLMIDHAFGGLGLHRIGLTVFSFNERAIRSYRSVGFVVEGRAR
                     EAIWREGRWWDEISMSILDSDWTAMPGRIPPAAADEAEPAKPSGVMAGIFGRRR"
     gene            7386..7664
                     /locus_tag="VHM48_11610"
     CDS             7386..7664
                     /locus_tag="VHM48_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756105.1"
                     /translation="MSSEGPAADAARHDVRELIEAKGHVVDNARGVIARLDAAFAAGD
                     LSRTPALMQFLDDLEPALEQDDGQRLGGKSAEAARFILRAIDRELDRA"
     gene            complement(7777..9027)
                     /locus_tag="VHM48_11615"
     CDS             complement(7777..9027)
                     /locus_tag="VHM48_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2756106.1"
                     /translation="MPTPESDRPVAIRCGRLLDVESGAIDEARVIHVGGDGRVVAVSS
                     GDRIPNDAIVLDLGDLTVLPGLIDVHTHLVGDVQTAGVPSTTTSAAEEVLIGVRHARE
                     TIRAGFTTVRDLGPFRAFTDCALRDAIDRGDVVGPRMQCAGAFITAPWGGGDVVGLAR
                     DIVLPEELRFGVVRSVDEVRERVRRLLIGGATMIKCIGTGAVLTRGGVPGAPELSEAE
                     IRAAVEEAGWYGTHVAVHAHGAEGAKRAIRAGARSVEHGSYLDDEAIAMLADTGTFFS
                     VDLYDGEWALGPDGPTDWPAETMRKLRESQVTAEAVMAKAIARGVRIAFGTDSGVYPH
                     GLNGRQFASYVRAGMTPLAAIRTATTNAAELMGWSDRVGSLAPGRFADLVAVAGDPLA
                     DVTELERPVVVAKGGRVVRDDRMG"
     gene            9104..9400
                     /locus_tag="VHM48_11620"
     CDS             9104..9400
                     /locus_tag="VHM48_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2756107.1"
                     /translation="MDDDLLSIGRFARLAGLSVGALRHYDELDLLRPADIDRFTGYRR
                     YRRVQLETARTIARLRDLELPLDEIREVLAMDDPAEQRRRLATHRPGSRPGRTD"
BASE COUNT         1397 a         3393 c         3424 g         1320 t
ORIGIN      
        1 tcattccact gtcggcccgc gcccgcgacg atggcgctgt gcaggtcatc acgtcatccc
       61 gtgcctcgcg tccccccctc ggtgccgtcg ttgtcggtct cgtcgtcggg agcgtcctcc
      121 tggtcggcgg attgctcctc gcctgggtcg ccttcgcgac cccggtcctc accggcctga
      181 caccgccgac cgcgcgcccg ggccctgccc agctggcgct cggcggggtg atctggggct
      241 tcacgctcgt cgcgccgccg tcgttcgcga tcgtcggcgc gttgcgcctc ggccgtgtcg
      301 tccgggccat ctcggtgaag ccgcggaccc gggcgatgac gcgcgtcgcc gcggcggtcg
      361 gcgatcagta cagcgccgcc accgatgtcc ggttgcccga cgggcggatc gttcgcgacc
      421 tcgtgctcgg tccgttcggt ctcgcggtga tcaacgagct gccgccgccg cgagcaacgc
      481 gccacaccgg gatcagctgg gagatccggc gcagcgacgg acggtgggtc catttcgaga
      541 acccgctcga gcggaccgcg cgcgacggcg agcgcgtccg ccgctggatc gcctcgaccg
      601 agcgcgactt cgtcgtgaag gtctacgcag cggtggtgac ccccgacccg acgatcagcc
      661 ggacgcccgg ctgtgcggtc ttgacccccg agcagatccc cgcctggctg gcatccctgc
      721 cgtcggcccg tgccctgacc gccgaccggc aggtagagct ggtcgaggcg atccggacga
      781 tcgtctagcg ggcccggcct cgtccggact cgtgtcgtcg aggcggacgg cgcggtggga
      841 ctggtagggg agccaggact cgaacctgga accagcggga tataagcccg ccgctctgac
      901 cattgagcta ctcccccgcc gcgatcctac tcccatccgc agcagcgccg ggtcggcgac
      961 gggtcgaccg ggtcgggcta gttgtcgacc tggccgagga ttcgaccggc cagggcgcgc
     1021 aggctcaacg gccgcgggtg gaggcccggc tcttcgtctc gccggccgaa ccgccgatcc
     1081 ggccgcccgt gcgagccacc gggcgcgtac atatgggcgg gcaggctcgc ggcgatctcg
     1141 cgttcctcgt cggtctggac ggcctcgatc gggaggtcgt ccgggtcgaa gggacgcgcg
     1201 gccaccggga cgaccacggt cggtcgtgcg taggcaggag cgccgtagag cttgggcagc
     1261 gcgacgtgct gctcgtccat cgccacgaat gagtcctcgc gtgccggatc ggatacccga
     1321 tcaacccgat gatatcgagt ggagggtacc gcggaatggt acgaatgggc cattccataa
     1381 catgcgcatg acgccggaag tggtgccccg acgcacaacc cgcgatcggc cagtgtcggc
     1441 cgtcctcagc cgatcagcgg cgccaggacc tgcgcgacga cgaccaggac gagatagcag
     1501 acggggaccg ccgcaaagaa gccgagtgcc gcgctccacg gcgagcgcgt cgtcgacagc
     1561 cccgagccgc gcggctgggg aacgaccagc caggtgacca cgctcccgac gacgagcaca
     1621 gtgacgatgt cgagggcgag gtacagcgtc cgcaggtcgc cgcccggcag gacggctcca
     1681 tggcgcagtg ccacgtcata ggcgaggtag ccgaggccga ggatgatcgc cacgaccagg
     1741 ctcgagagca ccgcccggac caccggccgt ggcgcgttcc ggaactggcg ccagcggatc
     1801 tcgagccagc tgcgttggcg aacctcgcga cggggaacgg gcgcgggtcg ctcggtgccg
     1861 ggagtggcct gacgctcgcc tggctcgggc gccgcggccg gaacgctcac ccttctccct
     1921 ggccgaggat cacgaggcgc ggccggggcg gcagggacgc cggcgtgtgg cttgccagca
     1981 gctcgacggt ggcggcgacg cgctcgatct cgtcggtgct cgtgaagaac ccgacgctga
     2041 tccggatcgc atcgaggccg gggatggtcc gcgcgatcgc gaagatgcgg ccgttcagct
     2101 cggccagcgt ctcgtcggcg gtccaacccg cgatccggaa cgtgaccagg gtcgccatcc
     2161 ggtcgcgcgg tgtcagcagc tcgacgccgg cgatgcccgc caggcgatcc gcggccgccc
     2221 gcgccagcgc ctgaccgcgc tcgtggatcc acggcagccc gacgtacatc gacagccagc
     2281 cgcagctccg ggcgagaccc gtcacgcctg gccgatagtg acccgagtcg tcgaagcggc
     2341 gcgcgtcccg ccacgggacg gcctcggccg tcgagaggcg ctcgaacgtg aaccagctcg
     2401 cgttcgtcgg acggctcgcc tcgaggatcg ccgggttgca ccagagcgcg cccgtcccct
     2461 ccgggccgag cagccacttc tggccgggca cggcatagaa atcgacgccg agatcggcga
     2521 ccgacacggg gatcgcgccg accgcctggg cgccatcgac gatgacccgg gcaccccgct
     2581 cgtgggccat cgtcgcgatc tcgcggaggg gcagccgggc gccggtggtc cacaggacat
     2641 gggacagcgc cacgatcctc gtccgcggcg tgatcgcggc atcgaacgcg gcgagcgtcg
     2701 cctcgtcgac gccaccgtgc ccgacgtcgg cgaacaccag ctcggcgccc gaccggccag
     2761 ccgcgacatg cacgggcccc aggccgccgg gatgctccgc ctgggtcgtc acgatccggt
     2821 cgcccggctg gaggtcgatc gaccagaccg cggcgttcat cgcctgcgag gtcgaatgga
     2881 cgagcgcgat ctcgccgacg tccgccccga tcacggccgc gactgatgcg cgcgcctcgg
     2941 ccatccgctc gaggaagccc tcccagtagt cgggatgcga gcgcccgatc ctgagctcgt
     3001 agtcgacgag ctcggccatc gctgcggcgg tctcgtccgg catcggcccg accgacccgg
     3061 tgttcaggta gatcccggca ccgagggccg gcaatgcccc gcggacggcc gcgagcttct
     3121 cgtgatcggg caggaagggg gccaccaccc gcctatgata tcgaccatgt cctctcttcc
     3181 cggtcgtcgg ctggcgcgaa cccgtgcacc tggcggcctc gcccggcccg agctgctcgc
     3241 ctcgaccgac tggctggccg acgagctcgg ccgaccggat gtccggatcc tcgatgtccg
     3301 ttggcgaccg gacggcagcg cccacgacgt ctgggcggcc ggtcacatcc cgggtgcggt
     3361 gcatgtcgac tggcgggcgg acctgaccca tccgtcggag gacggcgaca cgctcctcct
     3421 ggccgggccg gaacaggtgg cccggacgct gtccgtggcc ggcgtggggg atggcgccac
     3481 cgtcgtggtg tacgacgaca cggtcagcct gttcgccgcc cggacctggt ggagcctgcg
     3541 ggtctacggc ctggaatcgg tccggatcct ggacggcggt tacccggcgt ggacggagga
     3601 gggccggccc acgtccaacg cgaccgcgcc gccgccgccg gcccacttca cgccacgcgc
     3661 ccagtcccgg atgcggctga ccacgaccga tgtccgggcg ctcctgtcgt caccggacgt
     3721 gacgctgctc gatgcccggg cgcccgccga ataccgcggt catgagggca ataccaagcg
     3781 gctcgggcac atcccgggcg cggtcaacgt gccggtcggg gcgatgaccc gggccggcag
     3841 ccagcatttc aaggtcggcg acgagctgcg cgagcacctg ttcaagtcga acatcgcccg
     3901 cggccggcgg atggtctgct acgacggttc gggggtcgcg gcggccaagc tcgcgttcgc
     3961 cctgacgctg ctcggacacg aggacgtggc cgtgtatgac ggcggctggg ccgaatgggg
     4021 caaccgattg gatctgcctg tcgacaggta gctttcgcgc cttcgcggcc gaacgcggca
     4081 ctgtccccgc gcggggcggt ttgcgtcagg tgcacatgtc tgcagaagac tgccgctctc
     4141 gccgcaatgg cacgtcaatt ccatgcaccg ggcgaaacga atcagcgcga ctggcgggct
     4201 tcgacgatga cccatgccat tccttgcgct gcacacaaca agggacacga atccgggacc
     4261 ttcgcatccg acgtgtggat caggcgcaac gtcgagccga gtccgtgcgt gggacgcaac
     4321 ctgtcgaccc aacgcacgcc gatcgaaccc cgcggcgggc ccggcgaagc gccgcgcacc
     4381 tcaggttggg gtcggtccgc cctggcggct gcggtcgagg ccggcccagg ggtcgacctc
     4441 gcggtgcggc gcgagaatct gcgcccggtg gtggttgatc gccaccatcg ctgcggtggc
     4501 ccgcgcctcg ccgaccgaat cggaccagta ggtggcgtca gtgacgggga tgaaccgccc
     4561 ggtgagctcg ctgagcgcct cgcgcaggtc acgctccggc agcaggtgga tgcgaccggt
     4621 gacacggaac ggcgggatcg agatcaatgc ctgctcggac accttcggcg tccgcaggtc
     4681 cggccgcgcc tcgacgctcg tgtcggcgac ggcgaagagg acggcgccga ggttgacctg
     4741 ggcgaattcc gacttgacgg gcgtcacgcg ggcgccgtat tcgtcgacga ccgtatcggt
     4801 gaggacgagg aattcctcgt cggcctggtt gaggatgtcg gagatccggc cctgccggct
     4861 ccggatcgat cccctgatga cgaaggcgtc cgtatacagc gtcagggcca cgaggctcgg
     4921 ctcgcccccg agaagcaacg actcgaacat gggcgacagc ataacgggca cgacccgacc
     4981 ggacccgccc gattcgaccg ccggtcggac gacgccctac ttcctggcgc ccgcggtcac
     5041 cagctgttcg gcgccgggct ggaggccctc gtacagggcg cgcacggact cgagcgtgtc
     5101 gatctcgtcc ggcggcttgt cgggccgcag attggcgatc cgcgggaaac gcagcgaata
     5161 gcccgactgg tgccgtttcg agcgaacgat gacgtcgaac gcgacctcga ccacgaccgt
     5221 tggctcgacc tggcggtagc gcccgaagcg ctggatcgtg tgggcctcga accacgcggt
     5281 catctcggcg atctcggcgt cggtcaggcc gctgtacgcc ttgccgatgg tcacgaggcg
     5341 gtcgttgtcg gtgtcgcgga tggcgaaggt gtagtcggac aggacgccgt ggcgcttgcc
     5401 gtggccgaac tcgaccccga cgacgacgca gtcgatcgtc gccagcgcct tcttcatctt
     5461 cagccagccg aggccgcgcc ggccgggcga atagatcgag cgcgggtcct tgaccatcag
     5521 gccctcgttg cgccgctccc gcgcgccgag gaaggcgtcc tcgagcgcgc cctccgacgt
     5581 cacgctgacg aggcggctca ggacgaagac gccgccgccg ccggcaagcg gcagagccag
     5641 cccctcaagc aggcggcggc ggacctcgag cggctcgcgg aggagggcct cgatcggcgc
     5701 gtcgccaccg ccgcggccga gcccgaggac gtcgaacgcg acatagatca ccggcacctc
     5761 ggcccggatc gcggccgacg gtgacttccg cccgagccgg gcctggaggg cgatgaacgg
     5821 cagcacggcg ccgtcgcgcc aggcgaggat ctcgccgtcg aggatcccgg cccaggtcag
     5881 gccgcgggcg ccgtcgacga tctccgggaa ctggccgctg atgtcgtgga ggtcgcgcga
     5941 gtagagccgg acgtcgtcgc catcgcgatg gagctgggcc cggatgccgt cgtacttgtc
     6001 ctcgacccag accgggacgc cgagccgctt gacgatctcg gccgcatcct cggccggtga
     6061 ggcgagcatg aacttgatcg gatggaacag cgacaggctg gcctcgtcga gccggtcgtc
     6121 gcgggcgagg gcggccagct cgccgatgtc gccggtcagc atcccggccc acttgaccgc
     6181 gtcgagcggc cgcccgaacg ccttcgcgat cgccgcgtcg acgagcccct cgcgaagccc
     6241 gatccggagc tcgcccgaca ggaccttgac gatggacttg gccgtgagcg ggtccgaacg
     6301 ggcgaagagg tcgcgaagga ttccgctctt cgcggccggc ccggacgcct gctcgatccc
     6361 ggcgtaggcg gcggcgactt ccggcaatgt ggggctcatg gacgggtccg gtgcatggcc
     6421 ggcgagggtc aggacgtcct ccacggcctt gcccaggtcc gacgagcgat cgtacgcctc
     6481 gccgagagcg gcgcgcggga cgccggcaag gtcggtgacg gtcgtcgcga tcgccgacca
     6541 gccgaggccc gccgcccgct ggtccgcctc ggcaaacggc cgacccgtca ggaagacggc
     6601 ggcgatcggc agctcggcgg cgctcaggtc cgcgaagtag tcggcgagga gggcggtctt
     6661 ctcggaggtc cgggtcgtgg cgccgacgcg ctccgcgacc tcgctccagc gacgcatggc
     6721 ggggatggta taccgtcccc tccgatgagc ccccggacat ggttccccga gaccctcacg
     6781 ggcgagatgg tcgtcctccg ccggcacgcg cccgccaacc tccgcgcctt tctgcgctgg
     6841 tacgccgacc cggaggtcgt ccgcctgacg cggtaccagg acagtccgat gcggcccgac
     6901 gagatcgagc ggttctttgc cgcccgggcg ctggggtccg aatcgctggc gatggcggtc
     6961 cacctgcgcg acggcgaccg gctgatcggg acgtgcgcgc tgtcccagct cgatgcggac
     7021 aacggctcgg cgctgttcca catcacgatc ggcgagaagg acgcgtgggg gcagggccac
     7081 ggcaccgagg caacgcggct gatgatcgac catgcgttcg gcggtctcgg gttgcaccgg
     7141 atcgggctga ccgtgttctc gttcaacgag cgggcgatcc gctcctatcg gtcggtcggg
     7201 ttcgtcgtcg agggccgggc gcgcgaggcg atctggcgcg agggccgttg gtgggacgag
     7261 atctcgatga gcatcctcga ttcggactgg acggcgatgc cgggtcggat tccccccgcg
     7321 gcggcagacg aggccgagcc ggccaagccg tccggcgtca tggccgggat cttcgggagg
     7381 cgacgatgag cagcgagggt ccggcggccg acgccgcccg tcacgatgtc cgcgagctga
     7441 tcgaagcaaa gggccatgtc gtcgacaatg cgcgcggcgt gatcgcccgg ctcgacgcgg
     7501 cgttcgccgc cggcgacctg tcgcggacgc cggcgctgat gcagttcctc gacgatctcg
     7561 agccggcgct cgagcaggac gacggccaga ggctcggcgg caagagtgcc gaggcggccc
     7621 gcttcatcct ccgcgcgatc gatcgcgagc tggaccgggc ctgatcgggc atctctgacc
     7681 cggcacacga gtcagacggg ccagcagcga gcttggctct gccgcgatcc cgaccggacc
     7741 gtacacgctg gatgcggtct tgactcgacc ggtgagtcag cccatccggt cgtcgcggac
     7801 gacgcggcca cccttcgcga ccacgaccgg gcgctccagc tcggtgacgt cggccaacgg
     7861 gtcgccggcg accgcgacca ggtcggcgaa ccggccaggg gcgagcgatc cgacgcgatc
     7921 ggaccagccc atcagctcgg cggcgttcgt cgtcgccgtc cggatcgcgg caaggggcgt
     7981 catcccggcc cggacgtagg acgcgaactg gcggccgttg agcccgtgcg gatagacgcc
     8041 gctgtcggtg ccgaacgcga tccggacgcc gcgcgcgatc gccttcgcca tgaccgcctc
     8101 ggcggtcacc tgcgattccc ggagcttgcg catcgtctcg gccggccagt ccgtcgggcc
     8161 gtcggggccg agcgcccact cgccgtcata caggtcgacg ctgaagaacg tgcccgtgtc
     8221 ggcgagcatc gcgattgcct cgtcgtcaag gtacgagccg tgctcgaccg accgcgcgcc
     8281 ggcccggatc gcccgcttcg ccccttcggc gccgtgggcg tggacggcga catgcgtgcc
     8341 gtaccagccc gcttcctcga cagcggcccg gatctcggcc tccgagagct ccggcgcgcc
     8401 gggcacgccg ccgcgggtca ggacggcgcc cgtgccgatg cacttgatca tcgtcgcccc
     8461 gccgatcagg agccggcgga cccgctcgcg gacctcgtca accgagcgga cgacgccgaa
     8521 ccggagctcc tcgggcagga cgatgtcgcg ggcgagcccg acgacgtcgc cgccgcccca
     8581 cggcgcggta atgaacgccc cggcgcactg catccgcggc ccgacgacgt cgccgcggtc
     8641 gatcgcgtcg cgcagggcgc agtcggtgaa cgcccggaac gggcccaggt cacggacggt
     8701 cgtgaacccc gcccggatcg tctcgcgggc gtggcgaacg ccgatcagca cttcctcggc
     8761 ggccgaggtc gtggtcgacg gcacgcccgc ggtctggacg tccccgacga ggtgggtatg
     8821 gacgtcgatc aggccgggca ggaccgtcag atcgccgagg tccaggacga tggcgtcgtt
     8881 cggaatgcgg tcgcccgacg agaccgccac gacccgtccg tcgccgccga cgtggatgac
     8941 gcgggcctcg tcgatggcgc ccgactcgac gtcgagcagt cggccgcagc ggatcgcgac
     9001 gggtcggtcg gactcgggag tcggcatgtc cgaacgatag ccggacgacg tggtcgggcg
     9061 aggcacttga ctctcgacct gcccgagggt tcaggatggc gtagtggatg acgatctcct
     9121 gtcgatcgga cggttcgccc ggctcgccgg gttgagcgtc ggcgcgctgc gccactacga
     9181 cgagctggac ctgctccggc cggccgacat cgatcgcttc accggctacc gccgctaccg
     9241 gcgggtgcag ctcgagaccg cccggacgat cgcgcgtctg cgtgacctgg agctgccgct
     9301 cgacgagatc cgggaggtcc tggcgatgga cgacccggcc gaacagcgac ggcggctcgc
     9361 gacccaccgg cccggatcga ggcccggacg aaccgactga cccgcctgct ccacgtcgtg
     9421 gggcggatga ccgacggaaa ggacccgatc atgtcgaacg cgaccaccga ggcgacgacc
     9481 gcgacagccg tcgagctcga ctcggccacc caacgccagc tcggcgtcga cctc
//
LOCUS       DATAIW010000389         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_189994, whole genome shotgun sequence.
ACCESSION   DATAIW010000389 DATAIW010000000
VERSION     DATAIW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189994"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..584)
                     /locus_tag="VHM48_11625"
     CDS             complement(87..584)
                     /locus_tag="VHM48_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756108.1"
                     /translation="MIPVRRSRLPRARHIRPALLAAVAAVTVFVAGCGTISRTPAPAT
                     PAPFPGIAGQLSLHGIRVTGIVSGDAGCPDANLGKTAIGFDAVGLDQATSVRLHIYIF
                     RNRATYERLRASIDGCAATFVTDPETYESVETSPYVVAGQGPWGTTFKDNVRAAIAEA
                     AGTGD"
     gene            complement(656..3673)
                     /locus_tag="VHM48_11630"
     CDS             complement(656..3673)
                     /locus_tag="VHM48_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2756109.1"
                     /translation="MSVGFDTPQALLLLIPALLLTIGLHLASRRRVGLGRRRLALVLR
                     GFVLAFLVFALAGFRLVLPVDRLATVFVVDLSDSVGTAGREDALAWLRDSLKVMPEGD
                     VAGIVGFGRAALVERLPQEVREIDRIATTPVRSATDIGAALRLASALFPDDAQKRIVL
                     LSDGNDTTGSGQSEAALAAARGIQIETRAIGLGAADEVVVDRLTTPSTAKIGEELEAA
                     VDVSSTVAQPATIRLYADGGQIASKTVDLAAGSNHVTFLVKPTETGFHTFRAVVEAGR
                     DTFSQNNRADSDTIVNGEPRVLVLAGDEKVATELVAALKVEHQTVDSLIPEQLPAGID
                     GLLAWDSIVLVDVSRLRLSDAQLTGIQQYVRDFGRGLVMIGGPKSYGAGGYTDTPIEE
                     TLPVDMGVKDQEKQPDVALVVVIDKSGSMDACHCNSFNGGNPGGGTGIGGVRKVDIGK
                     EAILRAAAAMSARDQLGVVAFDESAHWVVQTQPLGGISDLRGAIGGIQPLGQTNIFAG
                     LDQAVTSLEGATATRRHIILLTDGWSTSGQYDAIIARMKAAGITLSTVGAGGGANPFL
                     QQLAKQGGGRFYPAVNPASIPDIFLKETQQVAGQQIVEEPFHPILTSQSPILRGIDAL
                     PQLLGYNGTTAKSAAQTVLVTPRDDPLLAQWQYGLGRSVAWTSDSTGRWAKGWIGWNG
                     FTKFFSQLVSWTFPGEETGGIEATFITEGNQTKLRVESVAEDGSPRDFYSTAVAITTP
                     DLVSKAIGLSQVAPGVYEVPLGEIDPGAYVIRVSQSRPGSTPLGRTLGLVAPTPAEYR
                     VLGTNESFLATLRSATAGRAILTAAEPWTHDLRTNASSTDLWPTLLILALLLWPLDIA
                     LRRVSIGRRELVDARRWLAGGWRRTMAPRTKEVAGMLAARDRAAGTAARAALLRRDEE
                     FAGQRAAHVAASPVDGPASPATPGVAARPAVAAMPAGPVAATPRSTSPSAPAGGRAPS
                     GPPSTPPAAAPLAPDEGDTLSRLRDAKRRSRER"
     gene            complement(3673..>4909)
                     /locus_tag="VHM48_11635"
     CDS             complement(3673..>4909)
                     /locus_tag="VHM48_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756110.1"
                     /translation="RPDLLATDDRAWAVVPPVRTRTILLVGEGDPYLETALLNLPNTA
                     LWGTTAEAYPKDVIRTDGTSWDLIIFEDNLPVTLPSTPILAIAPPKSSGLGAVVGTLT
                     NPGIATLGTDEPILRYVDLSTTHIAEAQQLTLPAWARTVIPGPKGAPLLYSGVRAGIP
                     TAVLAFEPRRSDLPLQVAFPILLANLTGELFGGSAAPTTAVKPGDPVVLVVPGGASGL
                     HVVAPDGRAIDLTPGAGSLSVTYTQTDQLGIYVATPIAAAGASGVPGASARPSASATI
                     APTGSGSPSGATPTPTPSGGLGQGIDPTAAIRFAVDLFDVDESAIAPGPNRVLEDLGK
                     ARPVAGASGAPGASGAPGNAADPVASRPNARDELWIPIVLLVLVGLCVEWSLYHRDAV
                     LRGWRGLTGRLRRNDRSGT"
BASE COUNT          681 a         1701 c         1835 g          692 t
ORIGIN      
        1 gcccggtcgt ccgggcgggc gtgggacgat ggtgcctgct gtcacaacgg tccggtcggg
       61 tgggtgccgg tctggtgtgg agcgggtcag tcgccggttc cagcggcctc cgcgatcgcg
      121 gcccggacgt tgtccttgaa cgtcgtcccc cacggaccct ggccggccac gacgtacggc
      181 gatgtctcga ccgactcgta ggtctcaggg tcggtgacga aggtcgcggc gcatccgtcg
      241 atggacgccc gcagtcgttc gtaggtcgcc cggttgcgga agatgtagat gtggagccgg
      301 actgatgtcg cctggtccag tccgaccgcg tcgaatccga tggcggtctt gccgaggttc
      361 gcgtccgggc agccggcatc gcctgacacg atcccggtga cccggatccc atggaggctc
      421 agctgaccgg cgatccccgg gaacggggcc ggggtcgccg gcgcgggggt ccgcgagatc
      481 gtgccgcagc cggcgacgaa caccgtgacg gcggcgaccg cggcgaggag ggccgggcgg
      541 atgtgccggg cacgaggaag ccgggaccgg cgaacgggga tcatcgggtg cgagcctaca
      601 ccggggacgc tgaccgcgcc cggtctgtca cccgtccgcc tgccccggtc cgaggctagc
      661 gctcgcggga ccgccgcttc gcgtcccgca gccgggacag cgtgtcgccc tcgtccggcg
      721 cgagaggtgc cgctgcgggc ggcgtcgacg gtggtccgct cggtgcccgt cccccagccg
      781 gggctgacgg tgacgtcgat cgcggcgtcg ctgcgaccgg gcctgcaggc atcgccgcga
      841 ccgccggcct cgccgcgacc cccggcgtcg cggggctggc cggaccgtcg accggactcg
      901 cggcgacgtg cgccgcccgc tgcccggcga actcctcgtc tcggcgaagc agggccgccc
      961 gggccgccgt accggccgcg cgatcgcgag ccgcgagcat ccccgcgacc tccttcgtcc
     1021 gcggcgccat cgtccgtcgc cagccgccgg ccagccagcg ccgcgcgtcg accagctcgc
     1081 gccgcccgat cgacacccgg cgcagggcga tgtcgagcgg ccagaggagc agggccagga
     1141 tcagcagggt cggccagagg tcggtcgatg aggcgttcgt ccggagatcg tgcgtccacg
     1201 gctctgccgc ggtgaggatg gcccggcccg cggtggcgga tcgcaacgtg gccaggaacg
     1261 actcgttcgt gccgaggacc cggtactcgg cgggggtcgg tgccacgagg cccagcgtcc
     1321 gcccgagcgg cgtcgagccg ggccggctct gtgacacgcg gatgacgtac gcgcccgggt
     1381 cgatctcgcc gagcggcacc tcgtagacac cgggcgcgac ctgcgacagc ccgatcgcct
     1441 tcgagacgag gtccggcgtc gtgatcgcga ccgcggtcga gtagaagtcg cgcggcgagc
     1501 cgtcctcggc cacgctctcg acccgcagct tcgtctggtt gccctcggtg atgaacgtcg
     1561 cctcgatgcc gccggtctcc tcgcccggga acgtccacga cacgagctgg ctgaagaact
     1621 tggtgaaccc gttccagccg atccagccct tcgcccagcg cccggtcgag tcggacgtcc
     1681 aggccaccga gcgtcccagc ccgtactgcc actgggccag gagcggatcg tcgcgcgggg
     1741 tgacgaggac cgtctgggcg gccgacttgg ccgtcgtccc gttgtatccg aggagctggg
     1801 gcagggcgtc gatcccgcgc aggatcggcg actgcgaggt caggatcggg tggaacggct
     1861 cttcgacgat ctgctgcccg gcgacctgct gggtctcctt gaggaagatg tcggggatgc
     1921 tcgccgggtt gaccgcgggg tagaaccgac cgccaccctg cttggccagc tgctgcagga
     1981 acgggttcgc gccgccgccg gcgccgacgg tcgagagggt gatgccggcg gccttcatcc
     2041 gggcgatgat cgcgtcgtac tggccggagg tcgaccaacc gtcggtcagc aggatgatgt
     2101 ggcggcgggt cgccgtcgcc ccctcgagcg atgtcacggc ctgatcgagg ccggcgaaga
     2161 tgttcgtctg gccgagcggc tggattccgc cgatcgcgcc gcgcaggtcg ctgatcccac
     2221 cgagcggctg ggtctggacg acccagtggg cgctctcgtc gaaggccacc acgccgagtt
     2281 ggtcgcgggc ggacatcgcc gcggcagccc ggaggatcgc ctccttgccg atgtcgacct
     2341 tgcggacgcc gccgatcccc gtcccgccgc cgggattccc tccattgaac gagttgcaat
     2401 ggcaggcgtc catcgagccg gacttgtcga tgacgacgac gagggcgacg tcgggctgct
     2461 tctcctggtc cttgaccccc atgtcgaccg gcaacgtttc ctcgatcggc gtgtcggtgt
     2521 agccgcccgc gccatagctc ttcgggccgc cgatcatcac gagtccgcgc ccgaagtccc
     2581 ggacgtattg ctggatgcct gtcagctggg cgtccgacag gcgcagccgc gacacgtcga
     2641 cgagcacgat cgagtcccac gcgaggaggc cgtcgatccc ggccggcagc tgctcgggga
     2701 tgagcgagtc gacggtctgg tgctcgacct tgagcgccgc gacaagctcg gtcgcgacct
     2761 tctcgtcgcc ggccaggacg aggactcgcg gctcgccgtt gacgatcgtg tcggaatcgg
     2821 cccggttgtt ctggctgaac gtgtcgcggc cggcctcgac gacggcccgg aacgtgtgga
     2881 agccggtctc ggtcggcttg acgaggaacg tgacgtggtt gctgccggcg gcgaggtcga
     2941 cggtcttcga ggcgatctgg ccgccgtcgg cgtagaggcg gatcgtcgcc ggctgggcga
     3001 cggtcgagct gacgtcgacc gcggcctcga gctcctcgcc gatcttggcg gtcgacggcg
     3061 tggtcagccg atcgacgacg acctcgtcgg cggctccgag cccgatcgcc cgggtctcga
     3121 tctggatccc gcgggcagcg gcaagggcgg cttcgctctg cccggagccg gtcgtgtcgt
     3181 tgccgtccga caggaggacg atccgtttct gggcgtcgtc ggggaagagc gccgaggcga
     3241 gccggagcgc agcaccgatg tcggtcgcgg agcggaccgg cgtcgtcgcg atccgatcga
     3301 tctcgcggac ctcctgcggc agccgctcga cgagcgccgc ccgcccgaag ccgacgatcc
     3361 cggcgacgtc accctcgggc atgaccttca ggctgtcgcg cagccaggcg agggcgtcct
     3421 cgcggccggc cgtcccgacc gagtcggaga ggtcgacgac gaagaccgtg gcgagccgat
     3481 cgaccggcag gacgagtcgg aagccggcca gggcgaagac gaggaacgcg agaacgaatc
     3541 cgcgcaggac gagggcgagc cgtcgccggc cgagcccgac ccgacgtcgc gaggccaggt
     3601 gcagaccgat cgtcagcagg agcgccggga tcagcagcag gagcgcctgc ggcgtgtcga
     3661 acccgacgct catcaggtcc cgctccgatc gttccgccga aggcgcccgg tcaacccgcg
     3721 ccatccccgg agcaccgcgt cgcggtggta cagcgaccac tcgacacaga gcccgaccag
     3781 gacgagcaga acgatcggga tccacagctc gtcgcgggcg ttcgggcggc tcgcgactgg
     3841 atcggcggcg ttgcccggcg ctccgctggc gcccggcgcc ccggacgcgc ccgcgacggg
     3901 gcgcgccttg ccgaggtcct cgaggacgcg gttcgggccg ggcgcgatcg ccgactcgtc
     3961 gacgtcgaag agatcgaccg cgaaccggat cgccgcggtc gggtcgatgc cctgaccgag
     4021 gccgccgctc ggggtcgggg tcggcgtcgc gccgctcggc gatccggagc ccgtcggcgc
     4081 gatcgtggcc gatgcggacg gccgggccga cgcaccgggc acgcccgacg cgcctgccgc
     4141 ggcgatcggc gtcgcgacat agatcccgag ctggtccgtc tgggtatagg tgacgctcag
     4201 gctgccggcc ccgggtgtca ggtcgatcgc gcgcccgtcg ggtgcgacga catggagccc
     4261 ggaggcaccg cccggcacga ccaggacgac cgggtcaccc ggcttgacgg ccgtcgtggg
     4321 cgccgccgat ccgccgaaca gctcgccggt caggttggcg agcaggatcg gaaacgcgac
     4381 ctggagcggc aggtcggatc ggcgcggctc gaaggccagg accgccgtcg ggatacctgc
     4441 ccggacaccg gagtagagga ggggagcgcc cttcggtccc ggaatgaccg tccgggccca
     4501 tgccggcagt gtcagctgct gggcctcggc gatgtgggtg gtcgagaggt cgacgtagcg
     4561 caggatcggc tcgtcggtcc caagggtcgc gatccccggg ttcgtcaacg ttccgacgac
     4621 cgcaccgaga ccgctcgact tcggcggcgc gatcgcgagg atcggtgtcg acggcagggt
     4681 caccgggagg ttgtcctcga agatgatcag gtcccacgag gtgccatcgg tccggatgac
     4741 gtccttcgga taggcctccg ccgtcgtgcc ccacaaggcg gtgttgggaa gattcaacag
     4801 ggccgtttcg agatagggat cgccctcgcc gacgaggagg atggtccgcg tcctgaccgg
     4861 cggaacgacg gcccacgccc gatcgtcggt cgcgaggaga tccggccgg
//
LOCUS       DATAIW010000390        15408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_115379, whole genome shotgun sequence.
ACCESSION   DATAIW010000390 DATAIW010000000
VERSION     DATAIW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115379"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VHM48_11640"
     CDS             <1..542
                     /locus_tag="VHM48_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756111.1"
                     /translation="YRVVAPAVTRAGGAPMVGWVAVASKTGEIWAQDVPLACPQVEGQ
                     VDLAEVVRLTSGTVADGGVSCFGQVPISIAASVAVTCAGPDAASTDTANWLAEPARMT
                     LRLTAGAASLDARVHPDLAGRTSCDPVSGTTWTVRGHFQDPAAEGCAAGAASVEVAEM
                     ARYRCRSIFVVTDLTPVAP"
     gene            complement(561..1400)
                     /locus_tag="VHM48_11645"
     CDS             complement(561..1400)
                     /locus_tag="VHM48_11645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756112.1"
                     /translation="MTAVIRPMTADDVEPAADLLRREAFGERHEFFAWALTQPSIAPI
                     VAEDAGRIVGTGCGSAHANAGWVGVIFVAHDDRGRGLGRRITRAVVDGLEGRGCRSQI
                     LIASPLGRPIYEREGFTVLERQIRFSIDGLPPDERPLDPGIRRFAPDDLAGVLALDRH
                     ATGEDRGAVLRSVLSPSTTFVAVDPAGSVRAYLARAPWRGGALIALDPDDALRLLEIR
                     RHSTGVSGKAGAGVLASNTVGRERLRAAGWLEEPGNVRMIRGEPLDWHSDAIWGQLNG
                     ALG"
     gene            complement(1408..1779)
                     /locus_tag="VHM48_11650"
     CDS             complement(1408..1779)
                     /locus_tag="VHM48_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2756113.1"
                     /translation="MINPDDVILKRFDEPDELREMILGRFELITVGGRTIGRATYQPG
                     WRWSSHVGPGLGQDRCPVEHVGLVLSGTATAAFADGRVVELRAGDAFYIPPIPHDSWV
                     VGDEPYVSLHLVGAEHYATDE"
     gene            1862..2167
                     /gene="gatC"
                     /locus_tag="VHM48_11655"
     CDS             1862..2167
                     /gene="gatC"
                     /locus_tag="VHM48_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2756114.1"
                     /translation="MPGLSRSDVEHVAHLARLGLTAEELTLLEGQLNHILEQYAKLAE
                     LDTDAIAPTAQTIELENILRADVVVPSLSVEDALRNAPVASGGYIVSPPILIGDSGE"
     gene            2202..3773
                     /gene="gatA"
                     /locus_tag="VHM48_11660"
     CDS             2202..3773
                     /gene="gatA"
                     /locus_tag="VHM48_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX2756115.1"
                     /translation="MTDLTRLLAHEMTNRLKSGEVSSRELAIAHLDAAEAGNHALNAW
                     LTIDRAEALRQADVADARLAADGPDAAPHHPLLGVPVALKDLVSVKGGQCTAGSRILE
                     GYIAPYDAHITERLRSVGAVILGKTNMDEFAMGSSNEHSAYGPVANPWDLETVPGGSS
                     GGSAVAVAAYHSPLSIGTDTGGSIRQPAALTGIVGIKPTYGRVSRYGIVAFASSLDQI
                     GPFARDVRDAAALLHAVAGRDERDSTSAPVPVPDELLHLPASDDEAAGGLKGKRLGLP
                     REYFVAGMDPDSRRRIEEAVAALEGAGATVEEVSLPHTDYGLATYYIVAPAEASANLA
                     RYDGVRFGPRLGDSDVLGNYLATRGRGFGPEVKRRIMLGTYALSAGYYDAFYVKAQKV
                     RTLIKRDFDTLFDQGFDALVAPTSPTVAFRFGAKMADPVAMYLSDACTLPVNMAGLPG
                     ISIPCGLSEGMPVGLQLIGPAWSEPELFRLSRAFEALTAGAPWRGQEPAQIGLTTDPA
                     QPTPAERAASLTAGR"
     gene            3925..4728
                     /locus_tag="VHM48_11665"
     CDS             3925..4728
                     /locus_tag="VHM48_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756116.1"
                     /translation="MRRGPLALALVGTGVAMGSVAAFRAIVRPWYEHWGVEPDDAGRL
                     LPGDELIAGATGGDTRGITIDAPAAAIWPWLAQMGFGRAGWYSYDTMDMRGSSANAIV
                     PEWQTLAVGQTMRTWPGGGFEVVQIDPEHAVVLYLDDTIVARQEQEARATAVGGVGAE
                     PTTPGLAASAAIMRTQPRTFRASWAFVLDPIDESRTRLIERVRVEYPEIAAWNRLTGP
                     LFGIAVFLMTRRQLLGIKERVERLISRGPIKPAAEGLPVETGHQELVPA"
     gene            4852..6060
                     /locus_tag="VHM48_11670"
     CDS             4852..6060
                     /locus_tag="VHM48_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2756117.1"
                     /translation="MTDATVTSRAPLAERALAERVRVVPPSGIRRFFDILATMDDVIS
                     LGVGEPDFDTPERIVAAGVRSLHAGRTHYTSNYGTIELRRALAAHLEARYGVAYDPAT
                     ELLITVGASEAVDLALRATCDPGDEVILHEPSYVAYVPAVIFAGGSVVHVGTRLEDDF
                     ALDPAAVEAAITPRTKALFLGYPCNPTGAVLPDAVQDELAAIATRHDLLVYSDEIYDR
                     LAYGSYRHRAFSALPGMRDRTILIGGFSKAYAMTGWRVGYLAAPAAILEGIVKVHQYG
                     IMSAPTTAQDAALEAIVGGEAEVERMRVEYDRRRRLVVDGFNALGLRTFEPRGAFYAF
                     PEVTTATGLSSEAFAQGLLEEERVAVIPGSAFGPSGEGHVRACYATSYEQLEDALGRI
                     GRFVDRQRGA"
     gene            6062..8923
                     /locus_tag="VHM48_11675"
     CDS             6062..8923
                     /locus_tag="VHM48_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2756118.1"
                     /translation="MAAQSQSPLAAPTSTSRPADTALSTFLLTDIAGSTRLWEEHGDA
                     MGAALAIHDELLRSAITGNGGVVVKTMGDGMLAVFEATSAAVEAALHAQRALRDIAWG
                     RTGPLRVRMAVHSGTAESREGDFFGQALNRDARILAIGHGGQILLSAVAALLARDRLP
                     ADVELLDLGSHRLRDLDRPEQVFQVAVGDLPREFPALRSLSTRRSNLPMPLTSFVGRQ
                     RELAEVDRLLERARLVTLIGSGGTGKTRLMIEVAGRVAHRFEDGVWLAELAPLGDPGE
                     IGPEIARALGISEMAGRPTLDVVAEFVASKELLLVLDNAEHLIDGVARIADRLLAAAP
                     GLRILTTSREALAVPGEAVIQVPSLTCPAGIDHAEDADAGPSTELDEASATEAVALFT
                     ERASAVLPTFLLTGTNVSAVAEICRRLDGIPLAIELAAGRVAAMSPEEIAVGLGDRFR
                     LLTGGRRTAVPRQQTLHALIDWSWDLLTDADRRLLRRLSTFVGGWTVPAAARVAAEPG
                     EGESVMDTVDGLTRLVDRSLVIVDRGATTRYRMLETIRQYAREQLIKSGEAPDAAGRH
                     LRFFAAMADAAAPELRGPSMVDWLDRLDAEIENLGAALEWALEAEPETAIRMGIALLE
                     YWVARVPSPDNQARVVAAVAAARRMVAGPPDPTRDQRALAARLLGRAATKWSLSGQGD
                     VAIGWGEEAVALAIDLDEPSVLIDAMVGRSTATIFTGGPGDIHGWLQEVVRLATEAGD
                     TYSIAFAAAGLAISLAEFDPIDIDRLSAVGVEAAHRTGSPHVIALTSMGRGTQLARSG
                     RFDEARASLQEAIDRFAETGDERLVEACRSELAHAVRRSGQVDEALALYRVSIGRWVR
                     SGNRGAVAHQLESIAFALIARRSLDRPARLLGAAAALRETAHSPMIRLEQHEHDEWLA
                     RLRESAEPALIDDAIAAGRRMSMADAVTLALILPEPAEP"
     gene            complement(8968..11343)
                     /locus_tag="VHM48_11680"
     CDS             complement(8968..11343)
                     /locus_tag="VHM48_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-lysosomal glucosylceramidase"
                     /protein_id="HEX2756119.1"
                     /translation="MTGRAKAASGPEQDPARIPSVAWRWPMGAPCPAVGHPHVSSRMI
                     DDGPWGGVPIGGLGTGSIGRTHRGDAARWHLEVGRHAFHPVAADGFAIFVGRSGGGSR
                     ATVLSTLRPATLPAWGWTLPEGGGMYHALFPRAWHTFEPDVLGVGLVGEQLSPVIGGD
                     LESSALPVGAFEWWIENPGPDRLTVGIVLSWQDPAADPAEPAPPGAWHEAAETSGAGG
                     AILHSAPAAPTGLRGTFAIAAAHERGVTITVRSRFDARSDAALWADFAADGRLEPSDD
                     HRPSGGGEAIGAAVAATVELAPGERRSLRFAVAWDLPIVEFGAGRQWWKRYTRAWGRT
                     GERAFALAAHALDQAPAWRAAIEAWQRPVLEADDRPDWYKAALFNELYYLVDGGTFWE
                     AGEVGSPEPDPADPGRFAMLECLDLDYAYYDTVDVDFYASFAVQRLFPELEARGIRDL
                     LAAIPVDDPEIVTIFASGLAARRKVGGTVPHDIGGPDDDPFHRPNRYRYQDVSGWKDL
                     APKFVLQLWRDAVDDPVGGAALIRDAWPTVDAVLTLLSDRDRDGDGLPEHDGLPDQTY
                     DTWPMHGPSAYGGSLWLAAVAAAAAMARRLGLAEDARRWQDWLHRGQAAFDARLWRGD
                     HYAYDDGGGPSSDSVMADQLAGQWYADVTGLGDLVPSERVAAALRTVHRLNVRGFAGG
                     TMGAVNGVRPDGTVDMSSEQSKEVWVGTTYALAAFMIGRGLTAEGWETARGAAAVTYE
                     RGLWFRTPEAYDRAGNYRASMYLRPLAIWAIEEALGRRAAPTSAAPESQRQ"
     gene            complement(11340..12350)
                     /locus_tag="VHM48_11685"
     CDS             complement(11340..12350)
                     /locus_tag="VHM48_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756120.1"
                     /translation="MIANEPAANVPDSTGPTYHGHATRRLINAHVWVDVLATAGPRIV
                     RLGLAGSAQNLLAETPDAGWETTNGRYELLGGHRLWFAPEDPERVAVPDGRGLEIELA
                     PNGIRLTGLAEQPTGLVRSMVLRLAPDRAGLQVCHELRNVGDVPLELAPWPITLLPLG
                     GTVLLPQAAAAPVHDVTPNRMLVLWPYTSWEDPRFRPRDGLVLLDAQAGPSLKVGYFN
                     DAGWVGYVRGGAVLVRRFEPQPGRPHPDLGCNVEVFIGGRFLELELLGPLTRLAPGAT
                     VALVEEWAIEAVDAALDEPDLRAMAARLGRALPASTSAAAAAAARGVPASRVGALGSI
                     EA"
     gene            complement(12347..14827)
                     /locus_tag="VHM48_11690"
     CDS             complement(12347..14827)
                     /locus_tag="VHM48_11690"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756121.1"
                     /translation="MRYGQFDDDQREYVIGRPDTPLPWINYLGSEDYFGIISNTAGGY
                     SFYRDARLRRLTRYRYNNAPLDVGGRYLYLRDDETGDAWSPCWQPTQDELDEYECRHG
                     LGYTTIASRRAGIRAETRYFVPPGENLEIWRTRITNERPVAATLSLFGTVEFCLWDAQ
                     DDATNFQRNYSIGEVEVEPGVIYHTTEYRERRNHFAFFACSDASAGFDTSRDAFLGPY
                     RGWDRPLAVERGTTSGSIAHGWQPIGAHHVRLALAPGETREVIFLLGYAENPKDAKFD
                     PPGSGTLDKRGVRKTIATYLEPLAVEAAVTALRRRWDELLGALQVESGNEHLDRMVNI
                     WNAYQCMVTFNLSRSASLFESGIGRGMGFRDSTQDLLGFVHMVPDRARQRILDIAATQ
                     LPSGGAYHQYQPLTKRGNDAVGGGFNDDPAWLVLAVAAYVKETGDLAILDEAIPYDNE
                     PGTERPLDDHLRRAIDYTLDRLGPHGLPLIGRADWNDCLNLNCFSDTPGESFQTTQNR
                     AGGVAESVFIAGLFVLAANELAAIADHRGDGAECLRLRAAAERMVAAVDAHGWDGAWF
                     RRAYDFFGEPIGSAANDEGQIFIEPQGICILAGIGLQDGRAARALASVRERLATPHGI
                     VLQQPAFSTYQLRLGEISSYPPGYKENGGVFCHTNPWVMIAEAMAGNPDAALDYYLRI
                     NPSAREAISDVHRCEPYVYAQMIAGRDAPTHGEAKNSWLTGTAAWNYVAATQWIVGIR
                     PELEGLRIDPSVPADWPEIRVTRRFRGATYRIVIRRASAAAAGPASVTRLVVDGRPID
                     GNLAPLPSAPGVDVEVEAILGVPIEVAR"
     gene            complement(14835..>15408)
                     /locus_tag="VHM48_11695"
     CDS             complement(14835..>15408)
                     /locus_tag="VHM48_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2756122.1"
                     /translation="SSAVVAYGFARFRFPAKNILFIILIATIILPFQVTLIPTYAVFT
                     KLGWNGTWLPLIVPHFFANAFNVFLLRQYFLSIPRDLDEAAMIDGASPFRVLRSVILP
                     LSVPALTAVTLFHFFYSWNDFFLPLLYLSGKPDLQTLPIAIQQYNALYVSQPTLIQAA
                     ALMTMAVPVVVFFLAQRAFMRGVVITGVEK"
BASE COUNT         2213 a         5440 c         5564 g         2191 t
ORIGIN      
        1 ggtaccgcgt ggtcgcgccg gcggtgacgc gcgccggcgg tgcgccgatg gtcggctggg
       61 tcgcggtcgc gagcaagacc ggcgagatct gggcgcagga cgttcccctt gcctgtccgc
      121 aggtcgaggg gcaggtcgat ctggcagagg tcgtcaggct cacgtcggga acggttgccg
      181 atggcggcgt ctcctgtttc ggccaggttc cgatctcgat cgctgcctcc gtcgccgtca
      241 cctgcgcagg accggatgcg gcctcgacgg acaccgcgaa ctggctcgcg gagcccgccc
      301 ggatgacgct ccggctgacg gccggcgcgg cctcgctcga tgcccgcgtc caccccgacc
      361 tggcgggccg gacgtcctgc gacccggtgt cggggaccac ctggacggtc cgcggtcatt
      421 tccaggatcc ggcggcggag gggtgtgccg ccggcgccgc ctcggtcgag gtggccgaga
      481 tggcccgcta tcggtgtcgt tcgatcttcg tcgtcacgga cctcacgccc gtcgcgccct
      541 gaaccaggcc gattccagcc tcagccgaga gcgccgttga gctggcccca gatcgcgtcg
      601 gagtgccagt cgagcggttc gccgcggatc atccggacgt tgccgggctc ctcgagccag
      661 ccggcggcac ggagccgttc gcgtccgacc gtgttgctcg ccaggacgcc ggcgcccgcc
      721 ttcccgctga cgccggtcga gtgacgccgg atctccagca gtcggagcgc gtcgtccgga
      781 tcgagcgcga tcagcgcgcc gccgcgccac ggagcacggg cgaggtaggc gcgaacgctg
      841 ccggccggat cgaccgcaac gaacgtggtt gacggtgaga gcacggaccg caggacggcg
      901 ccgcggtctt cgccggtcgc gtggcggtcc agggcgagga cgccggcgag gtcgtccggc
      961 gcgaaccggc ggatgcccgg gtcgagcggg cgctcgtcag gtggcaggcc gtcgatcgag
     1021 aaccggatct ggcgctcgag gaccgtgaag ccttcccgct cgtagatcgg tcgaccgagg
     1081 ggcgatgcga tcaggatctg actgcggcac ccgcgtccct cgaggccgtc gaccacggcc
     1141 cgggtgatgc gccgaccgag accgcgaccg cggtcgtcat gggcgacgaa gatcacgccg
     1201 acccagccgg cattggcgtg ggccgagccg caaccggtcc cgacgatccg tccggcgtcc
     1261 tcggccacga tcggcgcgat cgacggctgg gtcagggccc acgcgaagaa ttcgtgccgc
     1321 tcgccgaatg cctcccgacg cagcaggtcg gcggccggct cgacatcgtc ggccgtcatc
     1381 ggccggatga cggcggtcat cgatgcctca ctcgtcggtg gcgtagtgct cggcgccgac
     1441 gaggtggagc gagacgtacg gctcgtcgcc gacgacccag ctgtcgtgcg ggatcggcgg
     1501 gatgtagaac gcgtcgccag cccgcagctc gaccacgcgt ccgtcggcga aggctgccgt
     1561 cgcggttccg gacaggacga gcccgacgtg ctcgacggga cagcgatcct gtccgagacc
     1621 gggcccgaca tggctggacc agcgccagcc cggctggtag gtcgcccggc cgatcgtccg
     1681 cccgccgacg gtgatcagct cgaaccgtcc gaggatcatc tcgcggagct cgtcgggctc
     1741 gtcgaagcgc ttgaggatga catcgtcggg gttgatcacg tggcagtcct ctcgttcagt
     1801 cgggtctggg cggagggtac caagcgaggc cggagcgggc ccgtcggata tgatcggcgc
     1861 catgcccggc ctgtcccgga gcgacgtcga gcacgtcgcc cacctcgccc gactcggtct
     1921 gaccgccgag gagctgaccc tcctcgaggg acagctcaac cacatcctcg agcagtacgc
     1981 gaagcttgcc gagctcgaca cggacgcgat cgcgccgacg gcccagacga tcgagctcga
     2041 gaacatcctg cgcgcggacg tcgtggtccc atcgttgtcg gtcgaggacg ccctccgcaa
     2101 cgcgcccgtg gcatccggcg gctacatcgt ctcgccaccc atcctgatcg gcgacagcgg
     2161 cgaatgacgg ccgtggccgc cacccgccgg cgacaacgcc gatgaccgac ctgacccgtc
     2221 tcctcgccca cgagatgacc aaccggttga agagcggcga ggtcagctcg cgcgagctgg
     2281 cgatcgccca cctcgatgcg gcggaggccg gcaaccatgc cctcaacgcg tggctgacga
     2341 tcgatcgggc ggaggcgctc cgccaggccg acgtcgccga cgcccgcctg gccgcggacg
     2401 ggcccgacgc tgcgccgcac catcccctgc tcggcgtccc ggtcgccctc aaggacctcg
     2461 tgtcggtcaa gggcggccag tgcaccgccg gttcgcggat cctcgagggc tacatcgccc
     2521 cgtacgacgc ccatatcacc gagcggctgc ggtcggtcgg ggcggtgatc ctcggcaaga
     2581 cgaacatgga cgagttcgcg atgggctcct cgaacgagca cagcgcgtac ggcccggtcg
     2641 ccaacccgtg ggacctcgag acggtcccgg gtgggtcgtc gggtggatcg gcggtggcgg
     2701 tcgccgccta ccactccccg ctctcgatcg ggaccgacac gggcggctcg atccgccagc
     2761 cggccgctct gaccgggatc gtcgggatca agccgaccta cggccgggtg tcgcgctacg
     2821 ggatcgtcgc cttcgccagc agcctcgacc agatcgggcc gttcgcccgc gacgtccgcg
     2881 acgccgccgc cctgctccat gccgtggccg gccgcgatga gcgcgactcg acctcggcgc
     2941 ccgtgccggt gccggacgag ctcctccatc tccccgccag cgacgacgag gcggccggtg
     3001 gcctgaaggg caagcggctc gggctgcccc gggagtattt cgtcgccggg atggatcccg
     3061 actcgcggcg tcggatcgag gaggccgtgg cggccctcga gggcgccggc gcgaccgtcg
     3121 aggaggtcag cctgccccac acggactacg gcctcgcgac ctactacatc gtcgccccgg
     3181 cggaggcgtc ggcgaacctc gcccgctacg acggcgtccg gttcgggccg cggctgggtg
     3241 attcggacgt cctcggcaac tacctcgcga cgcgcggccg cggcttcggg cccgaggtga
     3301 aacgccggat catgctcggg acgtacgccc tgtcggccgg ctactacgac gcgttctacg
     3361 tgaaggccca gaaggtccgg acgctgatca agcgcgactt cgacacgctg ttcgatcagg
     3421 gctttgacgc gctcgtcgcg ccgacgtcgc ccacggtggc gttccggttc ggggcgaaga
     3481 tggccgaccc ggtcgcgatg tacctgtccg acgcctgcac gctcccggtg aacatggccg
     3541 gcctgcccgg gatctcgatc ccgtgcggac tgtcggaggg gatgccggtc ggcctgcagc
     3601 tcatcggccc ggcctggtcg gagccggagc tgttccgtct ctcgcgcgcc ttcgaggcgc
     3661 tgacggcggg cgccccgtgg cgcggccagg agccggccca gatcgggctg acgaccgacc
     3721 ccgcccaacc gacgccggcc gaacgcgccg cctcgctcac cgccggccgc tgacccgggc
     3781 cggtccggga ccacggtccg gcccgcgctc gacgattggc cgagatgatc cattgccgac
     3841 gaatcgatcc gcaacgacgg tccatccggc actcgaccgg caccttcgtg cactgcacga
     3901 tcgaggccaa ggaggaatgt cgacatgcga cgtggaccgt tggccctggc actcgttggc
     3961 accggcgtgg cgatgggttc cgtcgccgcg tttcgcgcca tcgttcggcc gtggtacgag
     4021 cactggggtg tggaaccgga cgacgccggt cgcctgctgc ccggcgatga gctgatcgcc
     4081 ggagccacgg gcggtgacac ccgcgggatc acgatcgatg cgccggctgc tgcgatctgg
     4141 ccctggctgg cccagatggg attcgggcgg gccggctggt acagctatga cacgatggac
     4201 atgcgcggtt cgagtgccaa cgcgatcgtg ccggagtggc agacgctcgc cgtcggccag
     4261 acgatgcgaa cgtggccggg cggcgggttc gaggtcgtcc agatcgatcc ggaacacgcc
     4321 gtcgtcctct atctcgacga cacgatcgtt gcgcgtcagg agcaggaagc gcgagcgacc
     4381 gccgtcggcg gcgtgggcgc ggagccgacg acgcccggtc tggccgcatc ggcagcgatc
     4441 atgcggacac agccccggac gttccgggcc agctgggcgt tcgtcctcga tccgatcgac
     4501 gagagccgca cccgcctcat cgaacgcgtt cgcgtcgagt accccgaaat cgcggcatgg
     4561 aaccggctga ccggaccgct gttcggaatc gccgtattcc tcatgacgcg acgccagctg
     4621 ctcgggatca aggaacgtgt cgagcggctc atcagcagag gtccgatcaa gccggccgcg
     4681 gagggactcc cggtcgagac gggccaccag gagctcgttc ccgcttaggg ggagctccct
     4741 gcggggcccc gcgggcaggt caaccggccg gttcaaccgg tcgagccggc caccgcgggg
     4801 cctcgccctt tcgccacccg ggtcgtggga tcgcgtaggc ttcatgtcgt catgaccgat
     4861 gccaccgtca cctcgcgcgc cccgctggcg gagcgggccc tcgccgagcg cgtccgggtc
     4921 gtgccgccgt cggggatccg ccggttcttc gacatcctcg cgacgatgga cgacgtcatc
     4981 agcctcgggg tgggggagcc ggacttcgac acgccggagc ggatcgtggc cgccggcgtc
     5041 cgatcgctcc acgccgggcg gacccactac acgagcaact acggcacgat cgagctgcgc
     5101 cgggcgttgg ccgcccacct cgaagcacgc tatggcgtcg cctacgaccc ggcgacggag
     5161 ctgctgatca cggtcggcgc gtcggaggcg gtcgatctcg ccctgcgggc gacctgcgat
     5221 ccgggcgacg aggtgatcct ccacgagccg tcctatgtcg cctatgtccc ggccgtcatc
     5281 ttcgccggtg gatcggtcgt ccacgtcggg acccggctcg aggacgactt cgccctcgat
     5341 ccggccgccg tcgaggccgc catcacgccc cggacgaagg ccctctttct cggctatcca
     5401 tgcaatccga cgggcgcggt cctcccggac gcggtccagg acgagctggc ggcgatcgcg
     5461 acacgccacg acctcctcgt ctacagcgac gagatctacg accgcctcgc ctacggctcc
     5521 tatcgccacc gggcgttcag cgcgctgccc gggatgcgcg accggacgat cctgatcggc
     5581 ggcttctcga aggcctacgc gatgaccggc tggcgggtcg gctatctcgc agcaccggcc
     5641 gcgatcctcg aggggatcgt caaggtccac cagtacggga tcatgtcggc gccgacgacg
     5701 gcccaggacg cggccctcga ggcgatcgtc ggcggcgagg ccgaggtcga gcggatgcgg
     5761 gtcgagtacg accggcggcg gcgtctcgtc gtcgacgggt tcaacgcgct gggccttcgc
     5821 acgttcgagc cgcgcggcgc gttctacgcg ttccccgagg tgacgacggc gaccggcctc
     5881 tcctccgagg cgttcgccca gggtctgctc gaggaggagc gggttgcggt catcccgggc
     5941 tccgccttcg ggccgagcgg cgagggccac gtccgggcct gctacgcgac gtcctacgag
     6001 cagctcgagg acgccctcgg ccggatcggc cggtttgtcg atcgtcagcg gggcgcctga
     6061 gttggctgcc cagagccagt caccgctcgc ggcaccgacg tcgacgagcc ggccggccga
     6121 caccgcgttg tcgaccttcc tgctgaccga catcgccggc agcacgagat tgtgggagga
     6181 gcatggcgac gcgatgggcg ccgcgctggc gatccacgac gagctgctgc ggtctgcgat
     6241 cacaggcaac ggcggggtcg tcgtcaagac gatgggcgac ggcatgctcg ccgtcttcga
     6301 ggccacctcc gccgcggtcg aggccgcgct ccacgcccaa cgcgcattgc gtgacatcgc
     6361 gtggggccgg accggtccgc tgcgcgtcag gatggcggtt cactctggga cggccgagtc
     6421 gcgcgagggc gacttcttcg gccaggccct caaccgcgac gcgcggatcc tcgcgatcgg
     6481 ccacggcggc cagatcctcc tgtcggccgt tgccgccctc ctggctcggg atcgtctgcc
     6541 ggccgacgtg gagctgctcg acctcggatc ccaccgtctg cgcgacctgg accgccccga
     6601 gcaggtcttc caggtcgccg tcggggacct gccccgcgag tttccggcgc ttcgatcgct
     6661 cagcacgcga cggtcgaacc tgccgatgcc gctgacgtcc ttcgtgggcc gccaacgcga
     6721 gctggccgaa gtggatcggc tgctggagcg ggcccgcctc gtgacgctga tcgggagcgg
     6781 cggaacgggc aagaccaggc tcatgatcga ggtcgccggc cgcgtcgccc atcgattcga
     6841 ggacggcgtc tggctcgccg agctcgcacc gctcggagat cccggggaga tcgggccgga
     6901 gatcgcgcga gccctcggga tctcggagat ggccggtcga cccacgctcg acgtcgtggc
     6961 cgaattcgtc gcttccaagg agctgctgct cgtcctcgac aacgccgagc acctcatcga
     7021 cggcgtcgca cggatcgccg atcgcctcct tgccgccgct ccggggctgc ggatcctgac
     7081 gacgagtcgc gaggcgctgg cagtgcccgg cgaggcggtc atccaggtcc cctcgctgac
     7141 ctgcccggcc ggaatcgacc acgcggaaga cgcggacgcc gggccgagca cggagctgga
     7201 cgaggcgtcc gcgaccgagg ccgtggcgct cttcaccgaa cgagccagcg ccgtgctgcc
     7261 gacgttcctc ctgacgggga ccaacgtctc cgccgtcgcc gagatctgcc gtcgtctcga
     7321 cgggatcccg ctggccatcg agctggccgc cgggcgcgtc gccgcgatgt cgcccgagga
     7381 gatcgccgtc gggctgggcg atcggttccg cctcctgacc ggtggtcgcc ggacggccgt
     7441 cccgcgtcag cagacgctcc acgccctgat cgactggagc tgggatctcc tgaccgacgc
     7501 agaccggcgc ctcctccggc gcctgtcgac cttcgtcggt gggtggaccg tgcccgccgc
     7561 ggccagggtc gccgccgagc ccggcgaggg cgagtcggtc atggacaccg tggatggcct
     7621 gacccggctg gtggatcgct ccctggtgat cgtcgatcga ggtgcgacga cccggtaccg
     7681 gatgctggag acgatccggc agtacgcccg ggagcagctc atcaagagcg gggaggcgcc
     7741 cgacgctgcc ggtcgccacc tgcgattctt cgcggcgatg gccgatgccg cggcgcccga
     7801 gctgcgcggc ccgtcgatgg tcgactggct cgaccggctc gacgcggaga tcgagaacct
     7861 cggcgcggcg ctcgaatggg cactcgaagc cgagcccgag accgccatcc ggatggggat
     7921 cgccctgctc gagtactggg tcgcccgcgt tccgtcgccc gacaaccagg cgcgcgtggt
     7981 cgcggccgtg gccgcggctc gccggatggt cgccggtcca cccgatccga cacgcgacca
     8041 gcgggcgctg gcggcccggc tcctcggcag ggccgcgacg aaatggtcgc tgtccggaca
     8101 aggcgatgtc gcgatcggat ggggggagga ggccgttgcc ctggcgatcg acctcgacga
     8161 accgagcgtc ctgatcgatg cgatggtcgg acgcagcacg gccacgattt tcaccggcgg
     8221 gcccggcgac atccatggct ggctccagga ggtcgttcgt ctcgcgacgg aggcgggcga
     8281 cacctactcg atcgcctttg ccgcggccgg cctcgcgatc agccttgcgg aattcgatcc
     8341 gatcgacatc gatcggctgt ccgcggtcgg cgtcgaggcc gcccaccgga ccggcagccc
     8401 gcatgtcatc gccctgacct cgatgggccg cggcacgcag ctcgcgcgga gcgggcggtt
     8461 cgacgaggct cgcgccagtc tccaggaggc gatcgatcgg ttcgccgaga cgggcgacga
     8521 gcggctggtc gaggcctgcc ggagcgagct ggcccacgcc gtccgccggt ccggtcaggt
     8581 cgacgaggcc cttgccctgt atcgggtctc gatcgggcgg tgggtccgct cgggcaaccg
     8641 cggcgcggtc gcccatcagc tcgagagcat cgccttcgcg ctcatcgctc gccggtcact
     8701 cgatcgcccg gcgcgactcc tcggagccgc cgccgcactc cgcgagaccg cccacagccc
     8761 gatgatccgc ctcgagcagc acgagcacga cgagtggctg gcccggctcc gcgaatcggc
     8821 cgagccggcg ctcatcgacg acgcgatcgc cgccggccgc cggatgtcga tggccgacgc
     8881 cgtgaccctc gcgctgatcc tgccggaacc cgccgagccc tgagcttgca tcccccaggg
     8941 ttccgacccc ggcgccacga gcccgtgcta ttgacgctga gactccggag cggctgacgt
     9001 gggcgccgcc cgccggccca gcgcctcttc gatcgcccag atcgcgagcg gccggaggta
     9061 catgctcgcc cggtagttgc cggcgcggtc gtaggcctcg ggcgtccgga accagagtcc
     9121 ccgttcgtag gtcacggcgg ccgcgccgcg ggccgtctcc cacccctcgg cggtcaggcc
     9181 ccgcccgatc atgaaggcgg ccagcgcgta cgttgtcccg acccagacct ccttcgattg
     9241 ctcgctcgac atgtcgacgg tcccgtcggg gcgcaccccg ttgacggcgc ccatcgtgcc
     9301 cccggcgaag ccgcgaacgt tgaggcggtg gacggttcgg agggccgccg cgacccgttc
     9361 gctcgggacg aggtcgccga ggccggtcac gtcggcgtac cactgccccg cgagctggtc
     9421 ggccatgacg ctgtcggagc tcgggccgcc accgtcgtca taggcgtaat ggtcgccgcg
     9481 ccagaggcgg gcgtcgaagg cggcctgccc gcgatgcagc cagtcctgcc agcgccgcgc
     9541 gtcctcggcc agcccgaggc ggcgcgccat cgccgcggcg gccgcgacgg ccgcgagcca
     9601 caacgagccg ccgtaggccg acggaccatg catcggccag gtgtcgtagg tctggtcggg
     9661 cagcccgtcg tgctcgggga gtccgtcacc gtcgcgatcg cggtcggaga ggagggtcag
     9721 gacggcgtcc accgtcggcc aggcgtcgcg gatgagcgcc gcgccgccaa ccggatcgtc
     9781 gaccgcgtcg cgccagagct ggaggacgaa cttcggggcg aggtccttcc acccgctgac
     9841 gtcctggtag cggtagcggt tcggccggtg gaaggggtcg tcatcggggc cgccgatgtc
     9901 gtgcggcacg gtcccgccga ccttgcgtcg cgcggccagg ccggaggcga agatcgtcac
     9961 gatctccgga tcgtcgaccg ggatcgccgc gagcagatcg cggatcccgc gcgcctcgag
    10021 ctccgggaag aggcgctgga ccgcgaacga cgcatagaag tcgacgtcga ccgtgtcgta
    10081 gtaggcgtag tccaggtcga gacactcgag catcgcgaag cggccgggat cggcggggtc
    10141 cggctccggg ctgccgactt cgccggcttc ccagaacgtg ccgccatcga cgaggtagta
    10201 gagctcgttg aacagggcgg ccttgtacca gtcaggccgg tcgtcggcct cgaggacggg
    10261 ccgctgccat gcctcgatcg cggcgcgcca cgccggagcc tggtcgaggg cgtgggctgc
    10321 cagagcgaac gcccgctcac cggtcctgcc ccaggcacgg gtgtaccgct tccaccattg
    10381 ccggccggcc ccgaactcga cgatcggcag gtcccacgcg accgcgaacc ggagcgatcg
    10441 tcgctcgcct ggtgccagct cgacggtcgc cgcaaccgcg gcgccgatcg cttcaccgcc
    10501 gccgctcggc cggtggtcgt cggacggttc gagccggccg tcggcggcga agtcggccca
    10561 cagcgccgcg tcagaacggg cgtcgaagcg cgagcggacg gtgatcgtca cgccgcgctc
    10621 atgggccgcc gcgatggcga acgtcccgcg caggccggtc ggtgcagcag gagccgaatg
    10681 gaggattgct ccgccggcgc ccgacgtctc ggcggcctcg tgccacgcgc cgggtggcgc
    10741 cggctcggcc gggtcggccg ccgggtcctg ccaggacaac acgatcccga cggtcagccg
    10801 gtccgggcct gggttttcga tccaccactc gaacgcgccg accggcaggg cgctcgactc
    10861 gaggtcgccg ccgatgaccg ggctcagctg ctcgccgacg aggccgacgc ccaggacgtc
    10921 cggctcgaac gtgtgccacg cccgcgggaa cagagcgtgg tacatgccgc cgccttccgg
    10981 cagcgtccaa ccccacgccg ggagggtcgc tggtcggagg gtcgagagga cggtcgcccg
    11041 gctgccgcca ccggatcggc cgacgaagat cgcgaagccg tcggccgcca ccgggtggaa
    11101 cgcgtggcgg ccgacctcga gatgccagcg cgccgcgtcg ccgcgatggg tgcgcccgat
    11161 gctccccgtc ccgagcccgc cgatcggtac accgccccac ggaccgtcgt cgatcatccg
    11221 gctggacacg tggggatgac cgacggcggg gcagggagcg cccatcggcc agcgccaggc
    11281 gacggacggg atgcgagctg ggtcctgctc cggtccgctc gcggccttcg cccggccggt
    11341 catgcctcga tgctgccgag ggcgccgacg cgtgacgccg ggacaccgcg cgcggcggcc
    11401 gccgcggccg cgctggtcga tgccggcagc gcccggccga gccgcgccgc catggcgcgc
    11461 aggtcgggct catccagggc ggcatcgacc gcctcgatgg cccactcctc gacgagcgcg
    11521 accgtcgcgc cgggcgccag gcgagtcaac ggacccagca gctcgagctc gaggaatcga
    11581 ccgccgatga acacctcgac gttgcagccg agatccggat ggggccggcc tggctgcggc
    11641 tcgaagcggc ggacgaggac agcgccgccg cggacgtagc ccacccagcc ggcatcgttg
    11701 aagtagccga ccttcaggct cgggccggcc tgggcgtcga gcagcacgag gccgtcgcgc
    11761 ggccggaagc gcgggtcctc ccaggaggtg tatggccaca ggacgagcat ccggttcggg
    11821 gtgacgtcgt ggacgggcgc agccgcggcc tgcggcagaa ggacggttcc cccgagcggc
    11881 agcagggtga tcggccacgg ggcgagctcg agcggcacgt cgccgacgtt gcgaagctcg
    11941 tgacacacct gcaggccggc gcgatcgggc gcaaggcgca ggaccatcga ccggacgagc
    12001 ccggtcggct gttcggcgag gccggtgagg cggatcccgt tcggcgctag ctcgatctcc
    12061 agccctcgcc catcggggac cgccacccgc tccgggtcct ccggcgcaaa ccagagccga
    12121 tggccgccga gcagttcgta gcggccgttg gtggtctccc agccggcgtc cggcgtctcg
    12181 gccagcaggt tctgcgccga cccggcgaga cccagccgca cgatccgcgg gccggccgtg
    12241 gcaagcacgt cgacccagac gtgggcgttg atgagacgtc gggtggcgtg gccgtgatag
    12301 gtcgggccgg tcgagtcggg cacgttcgca gccggctcgt tggcgatcat cgggccacct
    12361 cgatgggaac cccgaggatc gcttccactt cgacatcgac gcccggcgcc gacgggagtg
    12421 gtgcgaggtt gccgtcgatc ggtcgtccat cgacgacgag ccgcgtcacc gacgcgggtc
    12481 cggcggcggc cgctgacgcg cgccggatga cgatgcggta cgtcgcgcca cggaaccgtc
    12541 gcgtcacccg gatttcgggc caatcggcgg ggacggacgg gtcgatacgc agtccttcga
    12601 gctccggccg gatcccgacg atccactggg tggcggcaac gtagttccag gcggccgtgc
    12661 cggtcagcca cgagttcttg gcctcgccat gcgtcggcgc atcgcggccg gcgatcatct
    12721 gggcgtagac gtacggctcg caccggtgga catcggagat cgcttcccgg gcggatgggt
    12781 tgatccgcag gtagtagtcg agcgcagcgt cggggttgcc ggccatggcc tcggcgatca
    12841 tcacccacgg attcgtgtgg cagaagaccc cgccgttctc cttgtacccg ggcgggtacg
    12901 acgagatctc gccgagccgc agctgatagg tcgagaacgc gggctgctgg agaacgatcc
    12961 cgtgcggcgt ggcgagccgc tcgcgaaccg acgccaatgc ccgggcggcc cggccatcct
    13021 ggagcccgat gcccgcgagg atgcagatgc cctgcggctc gatgaagatc tggccctcgt
    13081 cgttggcggc cgatccgatc ggctccccga agaagtcgta ggcgcgccgg aaccacgcac
    13141 cgtcccaacc gtgagcgtcg accgccgcga ccatccgctc ggcggcagcc cgcaggcgaa
    13201 ggcactcggc cccgtcgccg cggtggtccg cgatcgccgc cagctcgttc gcggccagca
    13261 cgaacaggcc ggcgatgaac accgactcgg ccacgccgcc cgcgcggttc tgggtggtct
    13321 ggaacgactc gcccggcgtg tcggagaagc agttcaggtt gaggcaatcg ttccagtcgg
    13381 cccggccgat gagtggcagg ccgtgcggcc cgagccgatc gagcgtgtaa tcgatggcgc
    13441 gccggaggtg gtcgtcgagc ggccgctccg tgcccggttc gttgtcgtag gggatcgcct
    13501 cgtcgaggat cgcgaggtcg ccggtctcct tgacgtaggc cgcgaccgcg aggaccagcc
    13561 atgccgggtc gtcgttgaag ccgcccccga cggcgtcgtt gccgcgcttc gtcagcggct
    13621 ggtactggtg gtacgcaccg cccgacggca gctgggtcgc ggcgatgtcg aggatccgct
    13681 gacgggcgcg gtccgggacc atgtggacga acccgagcag gtcctgggtc gagtcgcgga
    13741 agcccatccc gcggccgatc ccggattcga agagcgacgc ggaccgcgac aggttgaacg
    13801 tcaccatgca ctggtaggcg ttccagatgt tgaccatccg atcgagatgc tcgttgccgc
    13861 tctcgacctg gagggcaccg agcagctcgt cccagcgccg gcgcaacgcc gtgaccgccg
    13921 cctcgacggc gagcggctcg aggtaggtcg cgatcgtctt ccggaccccg cgcttgtcga
    13981 gggtcccgga gccgggcggg tcgaacttcg cgtccttcgg gttctcggca tacccgagca
    14041 ggaagatgac ctcgcgcgtc tcgcccgggg ccagcgcgag ccggacgtgg tgggcgccga
    14101 tcggctgcca gccgtgggcg atcgagccgg aggtcgtgcc gcgctcgacg gcgagcggcc
    14161 ggtcccagcc gcggtacggg ccgaggaacg cgtcgcgcga ggtgtcgaag ccggccgacg
    14221 cgtcggagca ggcgaagaag gcgaagtggt tccgccgttc gcggtactcg gtcgtgtgat
    14281 agatgacgcc gggttcgacc tcgacctcgc cgatcgagta gttgcgctgg aagttcgtgg
    14341 catcgtcctg ggcgtcccac aggcagaact cgacggtgcc gaacaacgac agcgtggccg
    14401 ccaccggccg ctcattcgtg atccgcgtcc gccagatctc gaggttctcg cccggcggca
    14461 cgaagtagcg cgtctccgcc cggatcccgg cccgccgcga ggcgatcgtg gtgtagccca
    14521 gtccgtggcg gcactcgtac tcgtcgagct cgtcctgggt cggctgccag cacgggctcc
    14581 aggcgtcgcc ggtctcgtcg tcgcggaggt acaggtagcg cccgccgaca tcgagcggcg
    14641 cgttgttgta gcggtagcgg gtcagccgcc ggaggcgggc atcgcgatag aacgaatagc
    14701 cgccggccgt gttggagatg atcccgaagt aatcctcgct cccgaggtag ttgatccagg
    14761 gtaggggagt gtcgggccgg ccgatgacgt attcgcgctg gtcgtcgtcg aactgtccgt
    14821 agcgcacagt ccccctactt ctcgacaccc gtgatgacga cgccgcgcat gaaggcgcgc
    14881 tgggcgagga agaacacgac gaccggaacg gccatggtca tgagcgcggc ggcctggatg
    14941 agcgtcggtt gcgagacgta gagcgcgttg tactgctgga tcgcgatcgg cagggtctgg
    15001 aggtccggct tccccgacag gtacagcagc ggaaggaaga agtcgttcca cgagtagaag
    15061 aagtggaaga gggtgaccgc cgtgagggcc gggacggaca gcggcaggat gacggagcgc
    15121 agcacccgga acggactggc tccgtcgatc atcgcggcct cgtcgaggtc gcgtgggatc
    15181 gacaggaagt actggcgcag caggaacacg ttgaacgcat tggcgaagaa gtgcggaacg
    15241 atcagcggta gccacgtccc gttccagccg agcttcgtga agaccgcata cgtggggatc
    15301 agggtcacct ggaacggcag gatgatcgtg gcgatcagga tgatgaacag gatgttcttc
    15361 gcggggaagc ggaaccgggc gaacccatac gccacgacgg ccgaggac
//
LOCUS       DATAIW010000391         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_175122, whole genome shotgun sequence.
ACCESSION   DATAIW010000391 DATAIW010000000
VERSION     DATAIW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175122"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VHM48_11700"
     CDS             complement(<1..210)
                     /locus_tag="VHM48_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2756123.1"
                     /translation="MAVRQRRVELGEDRGRRLVNLTAAEFKAARLNAALSQEDVARAA
                     GISRPQYGRIERGRSPEVSLTTVARI"
     gene            complement(846..1724)
                     /locus_tag="VHM48_11705"
     CDS             complement(846..1724)
                     /locus_tag="VHM48_11705"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2756124.1"
                     /translation="MKRSSPLIPAADLLELVGSGDPDLRIVDCRWVLGVFGAGAAAYA
                     SGHLPGAVHLDLDTDLSDPRGYGAPGRHPLPTPAAFVARVARVGIADTSFVVAYDDVG
                     GWVAARLWWMLDVLGHPAVAVLDGGMAAWTASGGELTTDMPDPAPAELHLGTEWSRVI
                     DRAELKERLGSVVLLDARGAPRYRGETEPIDPVAGHIPTARNATVDDNLVEPGGPFRP
                     PAQLASHFHDLGADGTAGPVVTSCGSGVSALHHAIALRAAGLPDPILYVGSYSDWSRS
                     GEPVVTGPEPGDPPQG"
     gene            complement(1749..>2363)
                     /locus_tag="VHM48_11710"
     CDS             complement(1749..>2363)
                     /locus_tag="VHM48_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2756125.1"
                     /translation="TAGSADAADDDTLAGVRALAADLRLSWASRTHRGLFLRAEDFFG
                     FARRMATMRAELEAGLAQVLAEYADREAFARDLAASPYRKELAELRRRYGDGIDTHSH
                     GEAFLAFFQDRFVPGGTYFLDEPEAPLSPFRQLAFLSLVREAVAGGCQIVMATHSPIL
                     LACPDAAIWSFDEAPIRRVEYRDLEHVSLTRDFLNDPEAFLRHL"
BASE COUNT          350 a          846 c          866 g          301 t
ORIGIN      
        1 gatccgtgcc acggtcgtga gcgagacctc gggcgagcga cctcgttcga tcctgccgta
       61 ctgggggcgc gagattccgg cggcgcgcgc gacgtcttcc tgggacagcg cggcgttcag
      121 ccgggcagcc ttgaactctg ccgcggtgag gttcacgagc cgccggccgc gatcctcacc
      181 gagctccacg cgtcgttgtc gaaccgccat gtccggcagg atgaccgagc cggcttatct
      241 cgcggtcaac ggaccgggtg ccacccgatc aacgtggggt ggtgcgcggc cggggacgtt
      301 cggtagcgtc cgcggcggcg cggtgcggat gtccggtgcg actgggggag gccgcctccg
      361 tacgatggtc cgactcgtga acccgggtcg gccgcgccag ggtcgactcg atcggcgtga
      421 tgcggtcgtc ggtgccttca tcggggtatc gactgcattc atccgacgac ggccagcgag
      481 cacaccgagc ccaccgagtc ggccgacccc tcagacggca ccgagccgtc cgagtcgtcc
      541 gagtccgccg acccctcaga cggcaccgcg ccggcggagt ccgccgagcc ctccgacaat
      601 gcggagccct ccgacaacgc cgatccgacc gtgactaccg ctccgaccgt gaccaccgat
      661 ccgtgcgccg ccgagacccc gacggtcacg accaccatca cgctgccccc ggacctgatg
      721 gcgcaggtgc tcgccttcgt ggcctcgctc ctcgcctgag atcgaccagc cgcacgagcg
      781 gccacatcga cgacgcccct gcaccgcgca ggggcgtcgt cacgttccgg aggtcgcgcg
      841 cccccttagc cctggggcgg gtcgcccggc tcgggtcccg tgacgaccgg ctcgccggag
      901 cgggaccaat cgctgtacga gcccacgtag aggatcgggt cggggaggcc cgccgcccgc
      961 agcgcgatcg cgtgatggag cgccgatacg ccactcccgc acgacgtgac gaccggcccg
     1021 gccgtcccgt cggcgccgag gtcgtggaaa tgcgatgcga gctgggccgg cggccggaac
     1081 ggaccgccgg gctcgacgag gttgtcgtcg accgtcgcgt tgcgggcggt cgggatgtgg
     1141 cccgcgacag gatcgatcgg ctcggtctcg ccgcggtagc gcggcgcgcc ccgggcgtcg
     1201 agcaggacga ccgacccgag ccgctccttc agctcggccc gatcgatcac gcgtgaccac
     1261 tcggttccga ggtggagttc cgccggcgcc gggtccggca tgtccgtcgt gagctcgccg
     1321 ccggatgcgg tccaggcggc catcccgccg tcgaggaccg cgaccgcggg atggccgagg
     1381 acgtcgagca tccaccacag acgggcggcg acccagccgc cgacgtcgtc gtacgcgacc
     1441 acgaagctcg tgtcggcgat tccgacccgg gcgacgcggg ccacgaacgc ggccggcgtc
     1501 gggaggggat ggcgacccgg cgcaccgtat ccccgcgggt cggacaggtc cgtgtcgaga
     1561 tcgagatgga cggcgcccgg caggtgaccg gacgcgtacg cggccgcgcc cgcaccgaag
     1621 acgccgagga cccaccggca gtcgacgatg cgcaggtcgg ggtcgcccga gccgaccagc
     1681 tcgaggagat cagcggccgg gatgagcggc gacgaacgct tcatgcgcgg aggataccgg
     1741 ccgcgctctc acaggtggcg aaggaatgcc tcgggatcgt tgaggaagtc tctcgtcagg
     1801 gacacgtgct ccaggtcgcg gtactcgacc cgccggatcg gtgcctcgtc gaacgaccag
     1861 atcgccgcat cggggcaggc gagcaggatc ggcgagtggg tcgccatgac gatctggcag
     1921 ccgccggcga ccgcctcgcg gacgagcgac aggaacgcca gctggcggaa cggcgagagc
     1981 ggcgcctcag gctcgtcgag gaagtaggtg ccgcctggca cgaaccgatc ctggaagaac
     2041 gcgaggaacg cctcgccgtg cgagtgggtg tcgatgccgt cgccgtagcg tcgccggagc
     2101 tcggcgagct ccttgcggta cggcgaggcg gccaggtcgc gggcgaacgc ctcgcgatcg
     2161 gcgtactcgg cgaggacctg ggcgagccct gcttcgagct ccgcccgcat cgtcgccatc
     2221 cgccgggcga acccgaagaa gtcctccgct cggagaaaga ggccacggtg cgtgcggctc
     2281 gcccacgaca ggcgcaggtc ggccgcgagc gcccggacgc cggccagcgt gtcgtcgtcg
     2341 gccgcgtccg cggaccccgc ggt
//
LOCUS       DATAIW010000392         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_130424, whole genome shotgun sequence.
ACCESSION   DATAIW010000392 DATAIW010000000
VERSION     DATAIW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130424"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..713
                     /locus_tag="VHM48_11715"
     CDS             357..713
                     /locus_tag="VHM48_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756126.1"
                     /translation="MAGFGLFVGFGAPARGREVQASKVFGEVMAYYASLQASGEIESY
                     EVAILEAHGGELGGFILLRGDPERLARVRASAEFGRLIVRGGFAVDHVGVVSARLDSE
                     AARFVGESNGLTTDLT"
     gene            complement(754..1407)
                     /locus_tag="VHM48_11720"
     CDS             complement(754..1407)
                     /locus_tag="VHM48_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2756127.1"
                     /translation="MINEIPVKRSYRSPLRAAHASQTRRAIIDAATRLFVDAGYGATS
                     IDAIAEAAGVSRATVFTAVGGKAALLKTAYDVALAGDDEPVSLPDRADSRAIREEPDP
                     SRYLERYAGLVVELGGRVAAVYESVRGAASADREVRPVWDKIQQERRIGAAHVVADTA
                     SKGPLRDGIDPEGAADVVWVLNDPGLYHLLVNVRGWPAERYREWVAASLQHELLGDQ"
     gene            1488..1928
                     /locus_tag="VHM48_11725"
     CDS             1488..1928
                     /locus_tag="VHM48_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2756128.1"
                     /translation="MIADPRTASEPLAVVHRLQASTNAHDLDALVDCFTADYLSEIPL
                     HPSRSFRGSEQVRRNWGQILAGVPDLRSELVRYAIDGNVVWAEWEWTGTRRDGASHLM
                     RGTTILGVEGERIARARFYMEPVVDDGARIDAAVAQAFAPGSDR"
     gene            1925..2833
                     /locus_tag="VHM48_11730"
     CDS             1925..2833
                     /locus_tag="VHM48_11730"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2756129.1"
                     /translation="MIVVAGGSGTLGSLLVPLLVGRGEPVRVVTRDPAAAGRRLSGVE
                     LVAGDVTRPEDARRAVEGARVVVSAITGFASAAGLQAVDVEGNRILADAAARAGVEQL
                     VLVSIVQASADHPIELFRAKFAAEQAVRASGVGWTIVRPTAYLETWLGLIGGPLVATG
                     KTLVFGRGRNPINFVSALDVARLVDLAIADPSYRGKVVEVPGPENLTVNQLVAIIETA
                     CGRSGRVSHTPRAVMRLLSIALRPIDRMRARQVGTALFMDTRDMATDGRATRAGYPSI
                     PMTTAATVAERLFGATAGHLIQPSTT"
BASE COUNT          384 a          974 c         1024 g          453 t
ORIGIN      
        1 gtgaatgcgc agttcggcgg tgagcctact ctcgtcgaac cgctcccggg cccgccatgt
       61 ccgcccggac cctgcaccca ggtcgcgcag gacggtccct gtgacacggt ggtcttcgtg
      121 cgggtcggcg aggatgccta cttcgactac tcgctccagg gcggaccgta aggcaccggc
      181 gaaggcctac accgtggcga cggctcgtgc gtcgaggcgg ctggtgaggc aggggaccct
      241 ggccgatccg ccccttgtgt cccgggctgg cccggtggac aatgcgacgc agcgcgggcg
      301 cgaaattcca gcctgatccg gctttggtct ttgcgccccg atgggggggg ttcaccatgg
      361 ctggtttcgg gcttttcgtt ggattcggcg ctccggctcg cggccgcgag gtccaggcaa
      421 gcaaggtctt cggcgaggtg atggcctact acgcctcgct ccaggcgagc ggcgagatcg
      481 agagctacga ggtcgccatc ctcgaggctc acggcgggga gctcggaggc ttcatcctcc
      541 tgcgtgggga tcccgagcgg ctggcgcggg ttcgtgcctc ggccgagttc ggacgcctca
      601 tcgttcgagg cggcttcgcc gtcgaccacg tcggggtggt gtcggcacgg ctcgacagcg
      661 aggcggcccg cttcgtgggc gagtcgaacg ggctgacaac cgacctgacg tgagggtgag
      721 ccggcgctgg acgggccgct ggtcggagga cggctactgg tcgccgagca gttcgtgctg
      781 gagtgacgcg gccacccact cgcggtagcg ttccgccggc cagccgcgca cgttcacgag
      841 aaggtggtag aggcccgggt cattcaggac ccagacgacg tcggccgcgc cctccgggtc
      901 gatcccgtcc cggagcgggc ctttcgatgc ggtgtcggcg acgacgtgcg cggcaccgat
      961 ccggcgctcc tgctggatct tgtcccagac cggccgaact tcccggtccg ccgaggccgc
     1021 cccgcggact gactcgtaga ccgccgcgac ccggccgccg agttcgacaa cgagcccggc
     1081 gtagcgctcg aggtaccggc tggggtccgg ctcctcgcgg atcgcgcgcg aatcggcgcg
     1141 gtccggcaac gagactggct cgtcgtcgcc ggcgagcgcc acgtcgtagg ccgtcttgag
     1201 cagcgcggcc ttgcctccca cggcggtgaa gaccgtggcg cgactcacgc cggccgcctc
     1261 ggcgatcgcg tcgatcgagg tcgcgccgta cccggcgtcg acgaacagcc gcgtcgcggc
     1321 gtcgatgatc gcccgccgcg tctggctggc atgcgccgcc cggagcggcg agcgatagga
     1381 ccgcttgaca ggtatctcgt tgatcatcag actcacagta tagtcattac gcatacaggc
     1441 ttgcgaacag gctgagcggg tgacgccgaa ctggggggac cacgatcatg attgccgatc
     1501 cgcggacagc gagcgaaccg ctcgcggtgg tccatcggct ccaggcgtcg acgaacgccc
     1561 acgacctcga tgcgctcgtc gactgcttca cggccgacta cctgagcgag atcccgctcc
     1621 atccgtcgcg cagcttccga ggaagcgagc aggttcgccg caactggggc cagatcctcg
     1681 cgggcgtgcc ggacctccgc tcggagctcg tccgctacgc catcgacggg aacgtcgtct
     1741 gggcggaatg ggaatggacg ggcacccgcc gcgatggcgc gtcgcacctg atgcgcggga
     1801 cgacgatcct cggggtcgag ggcgagcgga tcgcccgggc ccgtttctac atggaaccgg
     1861 tcgtcgacga cggtgcccgg atcgacgctg cggtcgcgca ggccttcgcg ccgggctccg
     1921 accgatgatc gtcgtggctg gtgggtcggg caccctgggt tcgttgctcg tcccgctcct
     1981 cgtcgggcgc ggggagcccg tccgggtcgt gacgcgcgac ccggctgcgg cgggtcgcag
     2041 gctgtccggc gtcgagctcg tcgcgggcga cgtaacgcga cccgaggacg cccgccgggc
     2101 agtcgagggc gcgcgcgtcg tcgtctccgc gatcacgggc ttcgcctcgg cggctgggct
     2161 ccaggcggtg gacgtcgagg gcaaccgaat cctggccgac gctgcggccc gggcgggcgt
     2221 cgagcagctt gtcctcgtgt cgatcgtcca ggcgtcggcg gatcatccga tcgagctatt
     2281 tcgggcgaag ttcgcggccg agcaagccgt ccgggcgagc ggcgtcgggt ggaccatcgt
     2341 ccgcccaacc gcctacctcg agacatggct cgggctgatc ggcgggccgc tcgtggcgac
     2401 gggcaagacg ctcgtcttcg gccgcggccg gaacccgatc aacttcgtgt cggcgctcga
     2461 cgtggcgcgg ctcgtcgacc tcgcgatcgc cgatccgtcc taccgcggga aggtcgtcga
     2521 ggtcccggga cctgagaacc tgacggtcaa ccagctcgtc gcgatcatcg agaccgcctg
     2581 cgggcgttcg ggccgcgtgt cccatacccc acgggccgtc atgcgcctcc tttcgatcgc
     2641 gcttcggccg atcgatcgga tgcgcgcccg gcaggttggg actgcgctct tcatggacac
     2701 ccgcgacatg gccactgacg gtcgcgccac tcgggcggga tatccgtcga tcccgatgac
     2761 gaccgcggcg accgtcgccg agcgtctctt cggcgcgacg gctgggcatc tgatccagcc
     2821 gtcgacgacg tgacc
//
LOCUS       DATAIW010000393         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_86042, whole genome shotgun sequence.
ACCESSION   DATAIW010000393 DATAIW010000000
VERSION     DATAIW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86042"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHM48_11735"
     CDS             complement(<1..225)
                     /locus_tag="VHM48_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756130.1"
                     /translation="MDHSITARGTRGRKLLVLTMAFSLTLAASGAADAAYSYSNATRI
                     YACVNKVSKVARIVVPVNGKPACHPWEALVS"
     gene            complement(706..>2081)
                     /locus_tag="VHM48_11740"
     CDS             complement(706..>2081)
                     /locus_tag="VHM48_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX2756131.1"
                     /translation="FPAERVAAPSVDGNVLRFDLPSPESVVVWIDSLATLVVLPDPIE
                     AGAPLVGGPGVIDVIERGADPTGHALATMVLQAAIDAAAGGPGGGTVVLPRGVYRSGT
                     LTLKSNVTLYLARGALLQGSSDPADYPIDPGRIETASDTSLPPDVRYLGRTMTFSRLL
                     LIDRAENVRISGRGTIDGDGAFLRTRRNAAPNLLRVRESTNVRVDDVLFRNAAAWSLH
                     VLASRDVAFRNVKVINDRTNLNTDGIDIDMSTDVTIDRAFIHTKDDAVCVKATRNGDL
                     SGDPARINVTNSLVSAVDAGFKIGSETDAATLSDIVFEDGFVFDTGRAMSVVVRDGAT
                     VERVTFRRIRVGPNVDHLVEQVIGVRDPEAALGVIRDLAFDDVVAPSFVRPTSNRTWY
                     AQFRPGRPGPDVAVDVFEGADDTHAVDGLRLGGFVVNGQPLRDGPAARDVANLTIGPH
                     VRNVSFD"
BASE COUNT          322 a          696 c          742 g          321 t
ORIGIN      
        1 ggagacgaga gcctcccagg ggtgacaggc cggtttgccg ttgaccggta ccacgatccg
       61 ggcgaccttg gacaccttgt tgacgcaggc atagatccgc gtcgcattcg aatagctgta
      121 tgccgcgtcg gccgcgccgg aggcggccag cgtcaggctg aaggccatgg tcagaacgag
      181 cagcttgcga ccgcgagtcc cgcgcgcagt gatcgagtgg tccatcgaat ccctttcggt
      241 cggttgggtc gtccaatttg gtgacagctg ggggacgaac gacccgtttc cggccgtccg
      301 atggtcctcc ctcgatcaac gcagacgcac cgacccgatc ggacgcacgg gggccgtacc
      361 ttcggatcag gcgccacccc tgacccgggt cgaaggccgt cgacgcatac cgaggtcgcg
      421 ccgcgtccct ggacgcgaac gatcaccgcc gagttgaaga tcccgccggg aaccgccggc
      481 tcggcgctgg cgatccagac ggccgccggc ttccgctcac gggactgacg atcctcgggg
      541 tcggcctgct cgatccagcc gatgcgaccg gctggcgggt cgcgttcggc ctgcttgtcc
      601 tcggaccgag cgtcggcatc atcgcgatgt cccggctgcg ccgccgcccc gacgctcacc
      661 cggtggccaa cggccaccgc tgagccacgc tccgcccctg tcgcctcagt cgaagctcac
      721 gttccggaca tgcggtccga tcgtcaggtt cgccacgtcc cgcgcggccg gcccgtcgcg
      781 gagcggctgg ccgttgacga cgaacccgcc caaccggagg ccgtcgaccg catgcgtgtc
      841 gtcggcgcct tcgaagacgt cgaccgccac gtcgggaccg ggtcgtccgg gacggaactg
      901 ggcgtaccag gtccggttgc tcgtcggccg gacaaacgag ggcgcgacaa catcgtcgaa
      961 cgccaggtcg cggatcacgc cgagcgcggc ctcgggatcg cggacgccga tgacctgctc
     1021 gacgaggtgg tcgacgttcg gcccgacgcg gatgcggcgg aacgtgacgc gctcgacggt
     1081 cgcgccgtcg cggacgacga ccgacatggc ccggcccgtg tcgaagacga agccgtcctc
     1141 gaagacgatg tcggagaggg tcgcggcgtc cgtctcgctg ccgatcttga acccggcatc
     1201 gacggcgctc acgaggctgt tcgtgacgtt gatgcgggct gggtcgccgg acaggtcgcc
     1261 gttccgtgtc gccttcacgc agacggcgtc gtccttggta tggatgaacg cacggtcgat
     1321 cgtcacgtcg gtcgacatgt cgatgtcgat gccgtcggtg ttgagattcg tccggtcgtt
     1381 gatgaccttg acgttccgaa atgcgacatc cctcgaggcg agcacgtgga gcgaccacgc
     1441 ggccgcgttg cggaacagga catcgtcgac cctgacgttc gtgctttcgc gaacgcgcag
     1501 gaggttcggg gcggcgttgc gccgggtgcg gaggaacgcg ccgtctccgt cgatcgtccc
     1561 gcgaccgctg atccggacgt tctcggcccg gtcgatgagc agcaggcgtg agaacgtcat
     1621 cgtccggccg agatagcgga cgtccggcgg gaggctcgta tcggaggcgg tctcgatgcg
     1681 tcccggatcg atcggatagt cggcgggatc ggacgagccc tggaggagcg cgccccgagc
     1741 caggtacagg gtcacgttgc tcttcagggt caacgttccg gagcggtaga ccccgcgcgg
     1801 caggacgacg gtcccgccgc ccggcccgcc cgcggctgcg tcgatggcgg cctggaggac
     1861 catcgtcgca agggcgtgcc cggtcgggtc ggcgccgcgc tcgatcacgt cgatgacgcc
     1921 gggtccgccg acgaggggcg caccggcttc gatgggatcc ggcaggacga caagcgtggc
     1981 gaggctgtcg atccagacga cgacgctctc cggagacgga agatcgaacc ggaggacatt
     2041 gccgtcgaca ctgggcgcgg caacgcgctc ggcggggaag a
//
LOCUS       DATAIW010000394         7518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_86369, whole genome shotgun sequence.
ACCESSION   DATAIW010000394 DATAIW010000000
VERSION     DATAIW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86369"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..3228
                     /locus_tag="VHM48_11745"
     CDS             211..3228
                     /locus_tag="VHM48_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2756132.1"
                     /translation="MTADRTEAGRLEVPGPPEGQAAWHAVSLQDEVIRQSVDAIVVTD
                     ADLRVRVWNPAAERLYGIPAATAIGQSMAELVVTSDTTGEVIDGEAALAELAASGIWR
                     RRIIQRPRTGPLSQENVVIDTIVTLLRGSDGTTVGALGVNRDVTASALLESELAALGS
                     LVVATGRARTMAEVAQAALEILCRTTGAEAGLVTSAGPTYEATAHLGIHQATIDAVVS
                     YGQLDGPLARALKTPDSVVSADVVSAPIREDVRAAVIADGIEHLLVVGLRLSGRLTGI
                     LALGWRKRAPREPSRAIIQQAAALVTAAMENARLLTAVESGLNEERLLTRRMRALVEL
                     TRLPETTASSGSGIDRLMIEIATVIGADGSVFGEVEGDRLALREVDRIEWTEAEPLVN
                     RPLLSIPVASSLASGSAAVLFSLSERPTTPEGTAAGMGLGFLSGAAFAVRDVRRLSGI
                     LFALFRRPVEALEIDERTLDAIGRVLDISIANQRLREDIVAGERRYRDLFEASPDALL
                     VEGSETVIDANSAARKLFGDEIVGRAVMDLFVADREVIDRETLGPDGISGLLGTGRRL
                     DGSTFPEEVDLRPIEIAGQRRMLAIVRDLTERTRMQGELIQAQKMDAIGFLVAGVAHE
                     LNNPLTAIVGFSHLLRTDPNLPADLRSHADLLVQEANRTKGIVQNLLDFARLRPPERV
                     EMELRPLIDSVLGLQSYVLSRTDLTVAVDLAPDLPRIWVDRAQMQQVLINLTVNATQA
                     IRSVDRPGTIRIRSRSARNHDGPTVRIEIADDGPGVPPAIIDRLFMPFVTTKEPGAGT
                     GLGLSVSFGIVASHGGTLRHERNAEGGATFIIELPVERDEAGRAGSTSRATKTAAATS
                     GAGTSGAATSEAAAAAPAPATPTARPLRVLVLDDEPSIRDFLGRVLERNGYEPILADT
                     GALALEIVRTDPPDAILCDHRMAGMSGTEFHAAVMQVAPRLASRFAFMSGDVLNPELR
                     EFAEARGVHLLAKPFDIAAVASIVASLVANGPG"
     gene            complement(3262..4125)
                     /locus_tag="VHM48_11750"
     CDS             complement(3262..4125)
                     /locus_tag="VHM48_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2756133.1"
                     /translation="MTKERVLVAGAGLMGHGIAQVLAATGRSVALYEPDVARAVAGRD
                     RIAGNLERSVAKGRLDGDERAAILERIEPTADLAAAADVDFVVEAVFEDLAVKTALWS
                     EIDRLAPEAAIFASNTSSIAIDRLAAAVGGARRSRFVGMHFFSPVPVMPLIELIRGSA
                     TSDATEAAVRSIAAEMDKQVIVSADRPGFIVNRILMPFLAEAMRAYQEGLGTADDIDT
                     GAKVGLNHPMGPLELADFIGLDVCLGVMRVLHEGLGQEQFAPPAVLVELVEAGHLGQK
                     TGRGFHTYPRS"
     gene            complement(4209..5237)
                     /gene="meaB"
                     /locus_tag="VHM48_11755"
     CDS             complement(4209..5237)
                     /gene="meaB"
                     /locus_tag="VHM48_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2756134.1"
                     /translation="MAGSPDAPEAAARAADLVAAARAGDRRSLARLLTAVENRTAVAE
                     PALRELYPLAGAAHIVGITGPPGAGKSTLVAALIGEVRKAGRAVAVIAVDPSSPITGG
                     ALLGDRVRMQSYAGDRDVFIRSMAARGHAGGLASTTGAAAAVLDAAGFDLILIETVGT
                     GQSEVEVAAAADTTVVLEAPEMGDEVQAIKAGLLEVADLVVVNKGDKPGAQRTAAQLR
                     AMLVATGGPAAGADDDRPPRPRPKRPEVLITTAATGAGVPELLAALDRHRRTAAAAPG
                     ESARLARAEALVWSIVADRLRDRLHGPSLGGDTRVTLSAVAAHRLDPYAAADVLLERL
                     AGPAVLDG"
     gene            complement(5239..5643)
                     /locus_tag="VHM48_11760"
     CDS             complement(5239..5643)
                     /locus_tag="VHM48_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2756135.1"
                     /translation="MSDRPIKVLIAKPGLDGHDRGAKVLARGLRDEGFEVVYTGLRQT
                     PEMIATAALQEDVDVVGLSILSGAHMTLLPRITKLLRAEGLDDVLVTAGGIIPDDDVA
                     ALAEAGVARVFGPGTTIAEVAEYLRTNARPRD"
     gene            5731..6216
                     /locus_tag="VHM48_11765"
     CDS             5731..6216
                     /locus_tag="VHM48_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756136.1"
                     /translation="MGQSTEHSAPPARPRSRLPRAAARLAAVALGAVFLIPALVGLDQ
                     MQTRVPEGCFEFCDPQGGGRLLIALAVVGFLLAAAIWRKFLVALALGLCLSVLSTVVL
                     GAFVAGLAKSGELFRDPAGYATLVEIWSGSALATLFLVIALREEVADAGRREGSPSDG
                     R"
     gene            6388..7077
                     /locus_tag="VHM48_11770"
     CDS             6388..7077
                     /locus_tag="VHM48_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756137.1"
                     /translation="MEQITRDEAATLRENPGGGDGGGDAEVVGSEHSAPLGGLSSAKL
                     HVRSGLSSLRLRAGTDQRELYRAAFEGATPQVRLRDGRVLVQYRGIPFDWRKRNATFG
                     LNPTIPWTIEVVGGIQRVEADLRAIDVRRFDLAGGTERVQLELGRPRGEVPIRIVGGA
                     KTVRLERPRGVAVRLKVVGSTASATVDGRKLGSKGGESTVETSGWETSRDRVTLEVVG
                     GSRSIEIVDRP"
BASE COUNT         1058 a         2674 c         2716 g         1070 t
ORIGIN      
        1 gtcgaaccgc tcggcctcat cccgttcgat cgcggtcggt gcgaccgagc gccgggcaaa
       61 ctcgcgggtc gtgtcccgca gcaatcgctc ctcgtcggtc agcggccagg acaggggtag
      121 gtcctccgga tcgatcgtgg cgcggtcaac tcccctctcc ccccccgatc gagggggaga
      181 cggggcagcg cgcctgaata ggatagccac gtgacagccg atcgaaccga ggccggacga
      241 ctcgaggtgc ccgggccgcc cgagggtcag gcagcgtggc acgcggtttc actccaggat
      301 gaggtcattc gccagtcggt cgatgccatc gtcgtcaccg acgcggatct ccgggtccgg
      361 gtctggaacc cggcggcgga gcgcctgtac ggcattccgg cggcaactgc gatcggccag
      421 tcgatggctg agctcgtcgt cacgagcgac accacgggcg aggtgatcga cggcgaggcg
      481 gcgttggccg agctcgccgc cagcgggata tggcgacgcc gcatcatcca gcggccgcgg
      541 accgggccgc tgagtcagga gaacgtggtc atcgacacga tcgtgaccct cctgcgcggc
      601 tcggatggca cgaccgtcgg ggcgctcggg gtcaatcgcg acgtcaccgc ttcggccttg
      661 ctcgagtcgg agctggcggc cctcggctcg ctcgtcgtgg cgaccggtcg ggcgcggacc
      721 atggcggaag tcgcccaggc cgccctcgag atcctgtgcc ggaccacggg cgccgaggcc
      781 ggactggtga cctcggccgg ccccacctac gaggcgaccg cgcacctcgg cattcaccag
      841 gcgacgatcg acgccgtggt cagctacggc cagctcgacg ggccgctggc ccgggccctg
      901 aagacacccg attccgtggt ttccgcggac gtcgtttcgg cgccgatccg cgaggatgtc
      961 cgcgccgcgg tgatcgccga cggcatcgag cacctcctcg tcgtggggct ccggttgtcc
     1021 ggacggctca ccggcatcct cgccctgggc tggcggaagc gggctccgcg ggagccgtcg
     1081 cgagcgatca tccagcaggc ggcggccctg gtcaccgcgg cgatggagaa cgccaggctg
     1141 ctgacggccg tcgagagcgg gctgaatgaa gagcggctgc tcacgcgtcg gatgcgtgcc
     1201 ctcgtcgaac tgacgcgctt gcccgagacg accgcttcga gcggatccgg gatcgatcgg
     1261 ctgatgatcg agatcgccac cgtgatcggc gccgacgggt cggtcttcgg cgaggtcgag
     1321 ggcgaccgcc tggccctgcg cgaggtcgat cggatcgagt ggaccgaagc ggagccgctc
     1381 gtcaaccgcc cgctgctgag catccccgtc gcctcctctc tcgccagcgg ttccgcggcg
     1441 gtgctcttct cgctcagcga acggccgacc acgcccgaag gcacggccgc cggcatgggg
     1501 ctaggattcc tgtcgggggc ggcgttcgcg gttcgcgacg ttcggcgcct cagcggcatc
     1561 ctctttgcgc tcttccggcg tccggtcgag gctctcgaga tcgacgagcg gaccctcgat
     1621 gcgatcggac gcgtcctcga tatctcgatc gccaaccaac ggctgcgcga ggatatcgtc
     1681 gcgggtgagc gacgctaccg tgacctgttc gaggcctcac cggacgccct cctcgtcgag
     1741 ggcagtgaaa ccgtgatcga cgccaactcg gccgcgcgca agctcttcgg cgacgaaatc
     1801 gtcggtcggg cggtcatgga tctcttcgtc gccgatcgag aggtgatcga tcgcgagacc
     1861 ctgggcccgg acgggatcag cggcctgctc ggtacgggtc gccggctcga cggctcgacc
     1921 ttccccgagg aggtcgatct gcgcccgatc gagatcgccg gacaacgtcg gatgctggcc
     1981 atcgttcgcg acctgaccga gcggacacgg atgcagggcg agctgatcca ggcccagaag
     2041 atggacgcga tcgggttcct cgtcgcgggc gtcgcccatg agctcaacaa cccgctgacc
     2101 gcgatcgtcg gcttcagcca cctcctgcgg accgacccga acctgccggc ggacctccgc
     2161 agccacgccg acttgctcgt ccaggaggcg aaccggacga aggggatcgt ccagaacctg
     2221 ctcgacttcg cccggctgcg accgcccgag cgggtcgaga tggagcttcg cccgctgatc
     2281 gacagcgtcc tcgggctcca gtcctacgtg ctgtcgcgga cggatctgac cgtcgccgtc
     2341 gaccttgccc cggacctccc gcgcatctgg gtggaccgcg cccagatgca acaggtgctg
     2401 atcaacctga ccgtcaacgc cacccaggcg atccgcagcg tggatcgacc cggcacgatc
     2461 cggatccgct cccgatcggc gcggaaccac gacggaccga cggttcggat cgagatcgcc
     2521 gacgacgggc cgggtgtccc gcccgcgatc atcgaccgct tgttcatgcc gttcgtgacg
     2581 accaaggagc cgggcgcggg gaccgggctc gggctgtccg tgtccttcgg aatcgtcgcg
     2641 agtcatggcg ggacactgcg ccacgagcgg aacgccgagg gtggtgcgac cttcatcatc
     2701 gagctgccgg tcgagcgcga cgaggccggc cgggcgggtt caacgtctcg ggccacgaag
     2761 acggcggccg ccacgagcgg ggccggcacg agcggggccg ccacgagcga agcagcggcc
     2821 gctgcgccgg cgcccgcgac gccgaccgct cgaccgctcc gcgtgctcgt cctcgacgac
     2881 gagccgtcga tccgggactt cctcggccgg gtcctcgagc ggaacggcta cgagccgatc
     2941 ctcgccgaca cgggtgccct ggccctcgag atcgtgcgca ccgatccgcc cgacgcgatc
     3001 ctgtgcgacc accggatggc cggaatgagc gggacggagt ttcatgcggc ggtgatgcag
     3061 gtcgcgcccc gcctcgcctc gcgcttcgcc ttcatgagcg gcgacgtcct gaaccccgag
     3121 ctacgcgaat tcgccgaggc ccgcggggtc catctcctcg ccaagccgtt cgacatcgcc
     3181 gcggttgcgt ccatcgtcgc gtcgctcgtc gccaacggac ccggctgagc ggtcaaccag
     3241 cttcgaggcg cccgtcggga gtcagctgcg cgggtaggtg tggaagccgc gacccgtctt
     3301 ctggccgaga tgcccggcct ccaccagctc gacgaggacc gcgggcgggg cgaattgctc
     3361 ctgtcccagc ccctcgtgca gcacgcgcat gaccccgagg cacacgtcga ggccgatgaa
     3421 gtcggcgagc tcgagcgggc ccatcgggtg gttgagcccg accttcgcgc ccgtgtcgat
     3481 gtcgtccgcc gtgccaagcc cctcctggta ggcccgcatc gcctcggcca ggaagggcat
     3541 caggatccgg ttcacgatga agcccggacg atcggccgac acgatcacct gcttgtccat
     3601 ctcggccgcg atcgatcgga ccgcggcctc cgtcgcgtcg ctcgtcgccg atccccggat
     3661 gagctcgatc agcggcatga ccggcaccgg gctgaagaaa tgcatgccga cgaaccgcga
     3721 gcggcgggcc ccgccgaccg cggcggcgag gcggtcgatc gcgatcgacg acgtgttcga
     3781 ggcgaagatg gccgcctccg gcgccagccg gtcgatctcc gaccagaggg cggtcttgac
     3841 ggcgaggtcc tcgaagacgg cctcgaccac gaagtcgacg tcagcggccg cggcgaggtc
     3901 ggcggtcggc tcgatccgtt cgaggatcgc cgcccgctcg tcgccgtcga ggcgaccctt
     3961 ggcgacggat cgctcgaggt tgccggcgat ccggtcccga ccggcgacgg cccgcgcgac
     4021 gtcgggttcg tagagtgcca ccgagcggcc cgtcgcggcc aggacctggg cgatgccgtg
     4081 gcccatcagt ccggcgccgg ccacgaggac acgttccttc gtcatcgccg gtgagcctac
     4141 cgcttccggc cgtgagagga cggtcgagga cgcgtcgtca agcagccggg tcggcggcat
     4201 ggcgaatcct atccgtccaa gaccgcgggg ccggccagtc gctccagcag gacgtcggcg
     4261 gcagcatatg ggtcgagccg gtgggcagct acggcgctca gcgtgacgcg ggtgtcgccg
     4321 ccgagcgacg gaccgtggag tcgatcgcgc aggcggtccg caacgatcga ccagacgagg
     4381 gcttccgcgc gggccagccg ggccgattcg ccgggcgcgg cggcggcggt ccggcgatgg
     4441 cgatcgagcg ccgcgaggag ctccgggacg ccggccccgg tcgcggcggt cgtgatcagg
     4501 acctcgggcc gcttgggccg tggccggggg ggccgatcgt cgtcggcgcc ggccgccgga
     4561 ccgccggtgg cgacgagcat cgcccggagc tgggccgccg tccgctgggc gccgggcttg
     4621 tcgcccttgt tgacgaccac gaggtcggcc acttcgagga gtccggcctt gattgcctgg
     4681 acctcgtcgc ccatctcggg cgcctcgagc acgacggtcg tatcggcggc cgcggcgacc
     4741 tcgacctcac tctggccggt cccgacggtc tcgatgagga tcaggtcgaa gcccgcagca
     4801 tcgagcacgg cggcggccgc gccggtcgtc gaggcgagtc cgccggcgtg gccacgggcc
     4861 gccatcgagc ggatgaacac gtcgcgatcc ccggcgtagc tctgcatccg gacccggtca
     4921 ccgagcagcg ccccgccggt gatcggtgac gacgggtcga cggcgatgac cgcgaccgcc
     4981 cggccggcct tccggacctc gccgatcagc gccgcgacga gcgtcgactt gcccgctccc
     5041 ggtggccccg tgatcccgac gatgtgggcg gccccggcga gcgggtagag ctcgcgcagg
     5101 gcgggttccg caaccgccgt ccggttctcg accgcagtga ggaggcgagc gagcgagcga
     5161 cgatcgcccg cgcgggcggc ggcaacgaga tcggcggccc gggcggcggc ctcgggcgcg
     5221 tccggcgagc ccgccacgct agtcgcgcgg ccgagcgttg gtccgcaggt actccgccac
     5281 ctcggcgatg gtcgttcccg ggccgaagac gcgggcgacg cccgcctcgg cgagggccgc
     5341 gacgtcgtcg tcggggatga tcccgccggc cgtgacgagg acatcgtcga ggccctccgc
     5401 gcggaggagc ttcgtgatcc gcgggaggag cgtcatgtgg gcgcccgaca ggatcgacag
     5461 gccgacgacg tcgacgtcct cctggagggc ggcggtcgcg atcatctcgg gggtctggcg
     5521 gaggcccgtg tagacgacct cgaacccctc gtcacggagg ccccgggcga ggaccttggc
     5581 gccgcggtcg tggccgtcga ggcccggctt ggcgatgagc accttgatcg gtcgatcgga
     5641 catgggccga ttgtaggcgt gaggtgggcg gcgcgaatcg atgatgccgt ggcagttgca
     5701 gcggggagcc gtggcgagga tgctctgctc gtgggccagt caaccgaaca ctcggcaccc
     5761 ccagcccgtc cgcggagccg tctcccgcgc gcagccgctc gcctcgcggc ggtcgctctc
     5821 ggcgcggtgt tcctcattcc ggcccttgtc ggcctcgacc agatgcaaac ccgcgtgcct
     5881 gaaggctgct tcgagttctg cgatccgcag ggcggtggca ggctgctcat cgctctcgcc
     5941 gtcgtcggat tcctcctggc ggcggcgatc tggcggaagt ttctcgtcgc cctcgcgctg
     6001 ggactctgcc tcagcgtcct gtccacggtc gtcctcggcg cgttcgtcgc gggtctcgcg
     6061 aagtccggcg agctgttccg cgatccggcg ggttacgcga cgctggtcga gatctggagc
     6121 gggtctgcac tggccacgtt gttcctggtc atcgcccttc gcgaggaggt agccgacgcc
     6181 gggcgacgcg aaggatcacc gtcggatggc cgctgaccgg acggcatccc cgacccggcc
     6241 gaccggcggc gggtcatcgt cgaggcgatc cccgacaaga ttgcggcggt ccaggcgacg
     6301 ctcaatcggg tcggcagcgc gtccgccgac gagatcggcc gctacacgga cgcccagctc
     6361 gccctgatca ccgaattcct gacccggatg gagcagatca cccgcgacga ggcggcgacc
     6421 ctccgcgaga acccgggcgg cggcgatggt ggcggcgacg ccgaggtcgt gggatcggag
     6481 cacagcgcac cgctcggcgg tctgtccagc gcgaagctgc acgtccgctc cggcctgtca
     6541 tccctccgcc ttcgcgccgg caccgaccag cgcgagctct accgggcagc gttcgagggg
     6601 gcgacgccgc aggtccgact ccgcgacgga cgcgtcctcg tccagtaccg cggcattccg
     6661 ttcgactggc gcaagcggaa cgccaccttc ggcctgaacc cgaccatccc ctggacgatc
     6721 gaggtcgtcg gcggcatcca gcgcgtcgag gccgacctcc gcgcgatcga cgtccgccgg
     6781 ttcgacctgg ccggcggaac cgagcgcgtc cagctcgagc tcggccgacc acggggcgag
     6841 gtcccgatcc ggatcgtcgg cggcgcgaag acggtccgcc tcgaacgacc gcgcggcgtc
     6901 gccgtccgcc tgaaggtcgt cggctcgacc gcctcggcca cggtcgacgg gcggaagctc
     6961 ggcagcaagg gcggcgagtc aaccgtcgag acgtccggtt gggagacgag tcgcgaccgg
     7021 gtcacgctcg aagtcgtcgg cggctcgagg tcgatcgaga tcgtcgaccg accgtagagg
     7081 ccgagtgacc gcgcggcggg tcgcctgaag gtcgacgtgc ggtacgtccc ccggaccgtc
     7141 gcggtccgcg cctcggaccc cgcccagcag tcgcctcgaa cgccgggcgt gccatcccac
     7201 gccggaacgg cgtcgtcgtg gccacgcgcc gaacctcgag ggacgcaccg cacgctgggc
     7261 gttcagcccg ggcgtcaggc gcgcacggac tcacctcgcg agtcggatcg ggccgcagca
     7321 cgaggaggtc gggcgtcgtc gtgactcggc cgtgcgtgca gccgcttgat ctgactcaac
     7381 cactgggtca gggcggtcgc ccttgcgcgc gagccccgcg gggtgcgtca ggcgccgggc
     7441 gcccgccggt cgaggtgggc gaagaactcg gcgcgggtcc ggtcgcgggt ccggaagagc
     7501 ccgagcacgg ccgaggtc
//
LOCUS       DATAIW010000395        19418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_220083, whole genome shotgun sequence.
ACCESSION   DATAIW010000395 DATAIW010000000
VERSION     DATAIW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220083"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2915)
                     /locus_tag="VHM48_11775"
     CDS             complement(<1..2915)
                     /locus_tag="VHM48_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756138.1"
                     /translation="MTSGRVSGGPSRDAEAPTRTTWTRDGIGAIVAVLALGLVFRLFI
                     VQVNPLSGFGVDVGSFRAWAGNLAANGVGGFYDRPGFLDYTPGYLYVLWAVGVVGQAV
                     GNIGDLIKIPPILSDIALGWLVWSMVRELGGGRRAGLIGAALVVANPVSWFDSVLWGQ
                     VDSFGVVFLLLGVREVWRDRPERAAVFTVVAALIKPQLAILAPVLAVVTIRRAFWPTD
                     SAPVADGADGPDGFPARPGLAGALDRFWAWERRTDRPIRIMTTGLAGFLTAVVLCFPF
                     GLSVIEPGQNGEVLHSGLIEQVFKTAGGYPYASVNAYNPWALASIDGAGVAADGQWAC
                     DTIIRNPPAGHTSCPEAVMIGPFTAVVVGAALLAAAFLIVSLLVARRPTPLVILTGVT
                     ILAIAFFILPTRVHERYLFPFIAIGAILAAVSVRWRIAYVVLSVTTFLNMYVVLTTLY
                     PDNPGIDDWLGIGGTLRSTTAVQLIALTALAAALWAFAQLRPTAQRALDGELIAGGDD
                     DGVDAEGWVDEPDWPAHDHPSVPAGWRSQRSGAGAGSTVIASAIPPHGVDPGPADGVP
                     RQATFPTWTEPGSFAELGPIGWFRARLGQRPVRADRSHALHDEPPGRLDRLDLWILVV
                     LVVSVLGIRMFRLGEPYQMHFDEVYHARTAAEFLQSWRYGIDHDIYEWTHPHLAKYAM
                     AGGLIAWGDDRVNATSDLGVPVVDAAIERRTDDTEVAGRRDGDRVDLVTGAELRSYDL
                     VSRDLVATQPIDGAVAVAVDPSASQLFIGSSAGTIWTFDASALDGLRSGGSPGLLPEP
                     VEFGQVDGSIKRLFVPDDGSAVIVVTADDQVRSLDSVSGEVLGTVQLHAVADVGPGGT
                     APTLVGTPGSVADPHAVATRLATLLGGSAATYEVRLSGTAERLIIAGITDSAHQKTIQ
                     GAIDDGRLAGLSIDSLPQVAVADANGLELINPASGSLTSTIDVGGAAHGLALATVDRA
                     "
     gene            2945..4507
                     /locus_tag="VHM48_11780"
     CDS             2945..4507
                     /locus_tag="VHM48_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756139.1"
                     /translation="MDRRVAQGRSPLASDPPPSTLFVSIAVGAALVAIALVVYTLSHP
                     YRLYNHFEWQALAFLEGQAAIRYPVEATATSPGNAFFQDVLPVSTSRGLIPFPPLPAV
                     LLMPFVALWGLAANGQLVFAILGAVDVGIAWWMLGRLPIRTWVRVATTLFLGFGTVFW
                     YAAQIGTTWYQAHVLAVGLALAAIGVAIGADRDAARSEDDLDDAGAPVGAARTRFRLG
                     HVVPDARQFLAGLLFGLACTSRLTVAFAAPFFVLVGSGGSWQRRGWSAALGAGIPIGL
                     LIVYNVVSTGHVFNPGYQYLYELEAGFYTQLNYHIDWQIEDLRYLPQNFGIMFLNGPV
                     WLPTVVPSALDLGGPLCTDPAAVRGLFNPDCPLVLPKDTGMSILLTSPAYLLALPALR
                     WGFGLSRLVTAASLAVLIVAFVNLLHFSQGWVQFGYRFSLDFAPWAILLVAVGLERIA
                     ARTGPRDVVGPSWPGAGAIRGGATAPLGRAGAGAAYRVGARALVALAVALVVGSVLVN
                     FWGVVWGDVLGW"
     gene            4501..6249
                     /locus_tag="VHM48_11785"
     CDS             4501..6249
                     /locus_tag="VHM48_11785"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2756140.1"
                     /translation="MVTRIAPPDAEGQEPDAVRQGPDPALLAAWIAAIAIGVIALGLS
                     IWRLMPGVGFWDTAEFQTVLPVMGTAHPTGYPSYVLIGWLASVVLQPLGEAALRVNVL
                     SAILVGVGAAVTVDLARRLSGSLVFGIAAGLGIALTPIVWAIGTHADPHSLHFALVAI
                     ILWLLVRWEHARRGNPLEPAAAPAASDASDASDASDGRGGAGSSAPTRNADRWLLAAA
                     VVVGVSIGNHSLTLLLGPPIVLFVLAVEPGILQRRRFILANIAAFAIPTVLIRFEMVL
                     RAGWFRAPFVYADPSTFTGFWYVTLGAQFHGWLTDPFSNAPKRITDLVAIAVGQFGPL
                     AAILLVALAVTAIRQPRYALLSGMTMVLTCFFNSVYPDGAIDRYYIGPALIAWTWLAI
                     LGATIVDVFAPHDSIWSSRSGGRSTAWAALRPVGLVAVAGVLLAPSLLDLPGRAGSGA
                     IDQSGNRAAQAWTDDVLAALEPNAVVVSWWSYSTTLWYATIVEARRTDLCIIDDRNRL
                     DYNLGDLDQVIDRYIATRPVYLVRNGSSELGALAGRYTIASLATPHGDNVLQVSATAD
                     HGAPVAPPVGSSEPCR"
     gene            6246..7052
                     /locus_tag="VHM48_11790"
     CDS             6246..7052
                     /locus_tag="VHM48_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2756141.1"
                     /translation="MSERLATLSYFFPAHNEEANLEGLVAEALATLPSLADAFEIVIV
                     NDGSRDATGRIADGLTAAHPGIVRAVHHPVNLGYGAALRSGFRAARHDRVAFTDGDRQ
                     FRVEDVGRLIDRMAAGDRPDVVVGFRIKRADPLVRRLYARAYRLANRIFFGLAVRDVD
                     CACKLFRRDALEGLAVASDGAFFSAELLIKLRAAGRSIAEVGVPHHPRTAGSATGAKP
                     SVVFRAVRDFWRLRLRMWANGRQALGRGESILAANPADSAADSATGGRPA"
     gene            complement(7049..7654)
                     /locus_tag="VHM48_11795"
     CDS             complement(7049..7654)
                     /locus_tag="VHM48_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2756142.1"
                     /translation="MASEEPSASNGLVEMVVESVRVHMLSSRHVVILKESEHDRYLPI
                     WIGPWEASAIAMKLQGLTADRPLTHDLFASALENLGVRVERVVISTLSEETYHARLHL
                     ERDGTIFEIDSRPSDALALAVRTGGRIFASQAVLEQAALGGDASRTGDEEGGDVVPEG
                     SPLESTGEKIVDPRLDVFRDFVNSLDVDPNTGEGRTRSSDS"
     gene            complement(7662..8402)
                     /locus_tag="VHM48_11800"
     CDS             complement(7662..8402)
                     /locus_tag="VHM48_11800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2756143.1"
                     /translation="MIPLRDANPTHRRPVVTIGLIAVCVAAFAWELAVQSSEGESGLA
                     RAFRTFGLVPAALTAQLGGGGSDPLNLGPFAVLSSMFLHGDPFHIGFNMLFLWIFGNN
                     IEDRLGHVGFLAFYLAGGIVAAAAQVVVAPGSTVPVIGASGAIAAVLGAYLVLYPRAR
                     VLSLVYFGLFFQLIRLPAIVLLGLWFAVQVLGTLIASGPSGASGGVALFAHIGGFAGG
                     VIVGLLVRAVGSGGGTRSVRSTDRVGVG"
     gene            complement(8399..10702)
                     /locus_tag="VHM48_11805"
     CDS             complement(8399..10702)
                     /locus_tag="VHM48_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756144.1"
                     /translation="MAVRLQMKLGVIAEPDRSPNSPDTVVVVEPSIGAIARSKGHLYL
                     IVTSTIASARAQEATQLAAETIRGEYYYDESAGIRVCIEKAIASANKRLNHQRERLGL
                     HGTAANGPIGIGIAVVRGNELYVATIGPAEAYLIRQARLSTLPDPHRERGLPNSDVEP
                     DVWRGEISVGDSLVLVSPNVMSRLGPDELKDAMVTLHPQPAMEHLHHRFFAADGTGSD
                     AMIAFEATEVASTHRSRTLVPVRPAEPLAGAPDRSPIPLADNVTGGVAAVQASATRAR
                     SAAGGLFSRLVRRAQDILPHRRTSYRRVTPAAVKRETQRRAAMAILAFVLVMVALGTA
                     LYAVGGVGGTPQALSSAAAGADAIRKINANLDQVFGPGGVDLVDGDPQKAYQLLSAAW
                     DELAIATKANLPAATLDPLRAKTLGGLDRLFHVVPVAAATILSTANAKPPLDIQAMIL
                     GPQGVPYILDKATNSVYRVDLRTKKATVVFRWKKVVSAGVEGNPRLITTAARDLIIID
                     TKNVVWRWRPADQSGHGTTTKVKVVGAAGWGDDVTAVGTFLRDKRALLYNFYIVDPSR
                     RNIFAYPPGSDGNGYPSNAQPRLTVDRDVSKITELLIDGDIFAVDNGSITRFVRGQSE
                     GWQVQAPGTAAFAPGGDTLLRPAPVYAQIASASEKQTGLIYAWDRVSGRVVAYDKAKG
                     TFVSQYRLAGGSPAWQDVRGMYVVLGATPDAPSTLFWATKDGVMSAVLEGVKDEPIPG
                     PGASGSPRPSGGPSAPPKPSARASRAP"
     gene            complement(10859..11239)
                     /locus_tag="VHM48_11810"
     CDS             complement(10859..11239)
                     /locus_tag="VHM48_11810"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX2756145.1"
                     /translation="MSGRRRPMVRIGDLLPDAARGLGLEDELRLSRAMATFEQLVAER
                     VPAAAGACRVIRIEGFAIVVEADAPIVAQELRLRGPELLAAFAATPAGFGARELRVIV
                     ARHPGRGDGPGPGRGPGPIRTDRA"
     gene            complement(11236..12441)
                     /locus_tag="VHM48_11815"
     CDS             complement(11236..12441)
                     /locus_tag="VHM48_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX2756146.1"
                     /translation="MLESISIQGLRSYSALEAAFGPGPQLIVGPNAAGKTTLLEAIVL
                     LAWGRSHRTSSDAELVRWGSELARIEGVAGPETIEVALVRPAGAAGARKRIRVNGVAR
                     RAAGLVGLLRTVVFAPEEMLLVVGSPGLRRGALDQLATQRSAAYGRDLVSYARTLQQR
                     NSLLRAIREEQASRAELRFWDGSFLDAGGAIVAERLRLIDDLAGPLARAHAEIAPDEA
                     GAARLAISYATNAPALPRESPRDALARRLAETADKELWNGSTLVGPHRDDLVFSLDGR
                     AMSGFASRGQQRTAILAFKLAELDLLTEQDGRPPLLLLDDVFSELDPDRRSHLVRRIA
                     ALPQAFVTTTTLDDLDPALVRAATAWEVVPDGGGGAALRSPEASGNGPGRRPPAVAAA
                     PVGSVPEDR"
     gene            complement(12532..13647)
                     /gene="dnaN"
                     /locus_tag="VHM48_11820"
     CDS             complement(12532..13647)
                     /gene="dnaN"
                     /locus_tag="VHM48_11820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX2756147.1"
                     /translation="MKLSVMQENLARGLSVVSRAVSNRSLPVLTNVLLKTEDGGLKLT
                     ATNLEIGITYWVPGKIEIDGATSVPARLLTDLVNSLPGSEPISLELGDGETLHIRAGR
                     FESNIKGIPAEDFPTVQTAGERPITRVAQKVLRQALDETAFAAASDEARPILTGVLAK
                     FEGDQLTLAAADNYRIAVKTITILDPVEETSVVIPARALHELARVLADTDEPVSIVLA
                     HSRNQLLFHIDGIDLVTRLIDGQYPNYQSVLPASHATRAVLDREELLRAVRPAALIAH
                     ESANIVKLGVGLDGDNAITVSANAEIGDHVGRVEAAIEGDGTTIAFNARFLADVLTNV
                     DAEQFALELNGPLSPGVFKPIGDDRYVHVVMPLRTTS"
     gene            complement(14094..15458)
                     /gene="dnaA"
                     /locus_tag="VHM48_11825"
     CDS             complement(14094..15458)
                     /gene="dnaA"
                     /locus_tag="VHM48_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX2756148.1"
                     /translation="MDAKQVWRAALGELQVSLSPANFETWLRDTQLIDVDEQRFRIAV
                     PNGFAKDWLENRYRSLISQTLARIVGYSVQVEFTITTVEAPPAEDVPAGPVTQSPQVR
                     LEPGRVGGEGGSANLNPRYTFANFIVGSANRLAHAASLSVAERPGHAYNPLFLYGGVG
                     LGKTHLMHAIGSQVAARFPRKRIVYATSEKFTNEFITSIQQGKIDEFRARYRRIDLLL
                     IDDIQFIADKERTQEEFFHTFNAIHEDGKQIVLSSDRPPKAILTLEERLRSRFEWGLI
                     ADLTAPDLETRIAILRAKAEEGAVAITSDVIEFVARKVVSNIRELEGALNRIVAYASM
                     GAMPISIELAQAVLSNVLYNPKKRQVTPERIAKAVSDYYGVQMDQLKGQKRDKAIVMP
                     RQIAMFLMREETDVSLLRIGAELGGRDHSTVLHACDKITRESAVNDELRREIAAVREL
                     IYAD"
     gene            15912..16046
                     /gene="rpmH"
                     /locus_tag="VHM48_11830"
     CDS             15912..16046
                     /gene="rpmH"
                     /locus_tag="VHM48_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX2756149.1"
                     /translation="MKQTYQPKKRHRAKEHGFRARMRTPGGRRIVAARRARGRKRLTA
                     "
     gene            16082..16414
                     /gene="rnpA"
                     /locus_tag="VHM48_11835"
     CDS             16082..16414
                     /gene="rnpA"
                     /locus_tag="VHM48_11835"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX2756150.1"
                     /translation="MLSRPQEFAALQAGTVRSHPLLTARFLRTDLETTRFGLATGRKL
                     GGAVVRNRVRRRLREGLRAMAPSFQPGWDVLIIARPAIVETDYRNLVETLRRILRRGG
                     VVGGQADS"
     gene            16411..16617
                     /gene="yidD"
                     /locus_tag="VHM48_11840"
     CDS             16411..16617
                     /gene="yidD"
                     /locus_tag="VHM48_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX2756151.1"
                     /translation="MKRIGIGLIRLYRILFAWLPSSCRYEPTCSRYTEQAIDKYGLLR
                     GSWMGMRRIARCHPGHPGGYDPVR"
     gene            16637..17818
                     /locus_tag="VHM48_11845"
     CDS             16637..17818
                     /locus_tag="VHM48_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX2756152.1"
                     /translation="MTLPRLRSSLVRLLPLLAIVAVALLVAACAGGPAGSPGASGSPA
                     VTPTPQAAIPLVPQNPDNPFTLLAWLFTPLYQTFFILLVVLDKLTGNIAIAIILMTII
                     IRIILIPVFRRQTVSTKRTQMLQPEVKEIQRRFKGDRVKQQEAQRQLYAERGINPASG
                     CLPAILQIFLLIPMYQVFSQGLQNFNPQAMLDVFGFRIIDLHCAAAPIVTALGHVTNP
                     CLDPTAFSVNWGVPEVIIGTTGSMLSGLSLLAVISSLFQLVQSRMTLPPHDPAMADDP
                     QYKIQRQMAYIFPFISLIYGSFLPAGLFLYWITSTIFSIFQQYLIIGWGGMFPLFGWH
                     PGFAQGHAPRFPVVMPPPVDPKTRPPTSPLGGASGRAAQADKTIRHKERGRQGRRGRR
                     R"
     gene            17818..19014
                     /gene="jag"
                     /locus_tag="VHM48_11850"
     CDS             17818..19014
                     /gene="jag"
                     /locus_tag="VHM48_11850"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX2756153.1"
                     /translation="MSAYREFTGKSVEEALKVAREEFGVELNDLDFEILTAGSRGVLG
                     MGAEPARIVAAPRSALGGAAPKREAAATTPLPPAAPREDRAFGDGDRDRGPRRDDRGG
                     DRGGFRGGDRDRGPRRDERPAGGGGDRDRGPRRDDRAFGDRGPRNDPAPRDGTPVAEA
                     DAPIEASAPTEERPARPDRAAGGGDRDRGPRREDRAPRGDRPDRAPREGGPGTSALNA
                     TEAAEVKAARGSLAAERADLEAAEASPEALAAGKEILEELMRHIGFAVKVEVETGDTS
                     RLNVVADPDGREALGSLIGRKGERLSALQHLVNLMLSRRMGEWTRVLVDVEDYRGRRE
                     RQLRDLATRAAARVEETGKMIQLEPMPALERRWIHLALRDHPQVATQSIGEEPNRRIV
                     VLLRGG"
     gene            19171..>19418
                     /locus_tag="VHM48_11855"
     CDS             19171..>19418
                     /locus_tag="VHM48_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756154.1"
                     /translation="MTDLPNSAADRAAKASTPDIETPAGDPELAALVRREPRDDGDDP
                     SPAALVNLGRRPVSSAVPAAPAAGAPERPIAGAPAADET"
BASE COUNT         2779 a         6875 c         6883 g         2881 t
ORIGIN      
        1 gccctgtcga ccgtcgccag ggcgagcccg tgagccgcgc cgccgacgtc gatggtcgac
       61 gtcagcgagc cggaggccgg gttgatcagc tcgagcccgt tggcgtcggc gaccgcgacc
      121 tgcggcaggg agtcgatcga gaggccggcc agccgcccgt cgtcgatcgc gccctggatc
      181 gtcttctggt gggcgctgtc ggtgatgccc gcgatgatca gccgctccgc agtgccgctc
      241 aggcggacct cgtaggtggc cgccgagccg ccgagcagcg tcgcgagccg cgtcgcgacg
      301 gcgtgcggat ccgcgaccga tccgggcgtg ccgaccaggg tcggggcggt cccgcccggg
      361 cccacgtcgg caacggcatg gagctggacg gtcccgagca cctcgcccga gaccgagtcc
      421 agcgaccgga cctggtcgtc ggcggtgacg acgatcaccg ccgaaccgtc atcgggcacg
      481 aagaggcgct tgatcgagcc gtcgacctgg ccgaactcga ccggctccgg gaggaggccc
      541 ggcgagccgc cgctccggag cccgtcgagg gccgacgcgt cgaaggtcca gatcgtgccg
      601 gccgagctgc cgatgaagag ctgactcgcg ctcggatcga cggcgacggc gacggccccg
      661 tcgatgggct gggtcgcgac gaggtcgcgg ctcacgaggt catacgagcg gagctcggcc
      721 ccggtgacga ggtcgacccg gtcgccatcg cgccggccgg ccacctcggt gtcgtcggtc
      781 cgtcgttcga tcgcggcatc gacgaccggg acgccgagat cgctcgtcgc gttgacccgg
      841 tcgtcgcccc aggcgatcag gccgccggcc atcgcgtact tggcgaggtg ggggtgggtc
      901 cactcgtaga tgtcgtggtc gatgccgtag cgccagctct ggaggaactc ggccgccgtc
      961 cgggcgtggt agacctcgtc gaagtgcatc tggtacggct cgccgagccg gaacatccgg
     1021 atgccgagga ccgagaccac gaggacgacc aggatccaca ggtcgagccg gtcaaggcgt
     1081 cccggcggct cgtcgtggag ggcgtgcgag cggtccgccc ggaccggccg ctgcccgagg
     1141 cgggcgcgga accagccgat cggcccgagc tcggcgaagg atcccggctc ggtccacgtc
     1201 gggaacgtgg cctgacgagg gacgccgtca gccggtccag ggtcgacgcc gtggggcggg
     1261 atcgccgagg caatcacggt cgagccagca ccagcgccgg agcgttgtga ccgccagccg
     1321 gccggcaccg acgggtgatc gtgcgccggc caatccggct cgtcgaccca cccctcggcg
     1381 tcgacgccat cgtcgtcgcc gcccgcgatc agctcgccat cgagggcccg ctgggccgtc
     1441 gggcggagct gggcaaacgc ccagagcgcg gccgcaagcg cggtcagggc gatgagctgg
     1501 accgcggtcg tcgaccggag ggtcccgccg atcccgagcc agtcgtcgat ccccgggttg
     1561 tccgggtaga gcgtcgtgag gacgacgtac atgttcagga aggtcgtgac cgacaggacg
     1621 acgtaggcga tccgccagcg gaccgagacc gcggcgagga tcgccccgat cgcgatgaac
     1681 gggaagaggt agcgctcgtg gacgcgggtc ggcaggatga agaacgcgat cgcgaggatc
     1741 gtcaccccgg tgaggatcac cagcggggtg ggtcgccggg caaccaggag cgagacgatc
     1801 aggaacgcag cagccagcag ggcggcgccg acgacgaccg ccgtgaacgg gccgatcatc
     1861 actgcctcgg gacaggacgt gtggcccgcc ggcgggttgc ggatgatcgt gtcgcaggcc
     1921 cactggccgt cggcggcgac gccggccccg tcgatcgacg cgagcgccca cgggttgtag
     1981 gcattgacgc tggcgtatgg atagccgccg gcggtcttga agacctgttc aatcaggccc
     2041 gagtggagca cctcgccgtt ctgtccgggc tcgatcaccg acagtccgaa cgggaagcac
     2101 agaacgacgg ccgtcaggaa gccggccaga ccggtcgtca tgatccggat cgggcgatcg
     2161 gtccgccgct cccacgccca gaaccggtcg agggcgccgg cgaggcccgg ccgggcgggg
     2221 aatccgtcgg ggccgtcggc tccgtcggcg accggcgccg agtcggtcgg ccagaacgct
     2281 cgccggatcg tgacgacggc caggaccggg gccaggatgg cgagctgcgg cttgatcagc
     2341 gccgcgacga ccgtgaagac cgccgcccgt tcgggtcggt cgcgccacac ctcccggacg
     2401 ccgaggagga ggaagacgac accgaacgaa tccacctggc cccacagcac ggagtcgaac
     2461 cagctgacgg gattggccac gacgagggcg gctccgatca ggccggcgcg ccgaccgccg
     2521 ccgagctcgc ggaccatcga ccagaccagc cagccaaggg cgatgtccga caggatcggt
     2581 ggaatcttga tcaggtcgcc gatgttgccg accgcctgac cgacgacccc gaccgcccag
     2641 aggacataga gatagcccgg cgtgtagtcg aggaagccgg gccggtcgta gaacccgccg
     2701 accccgttcg cggcgagatt gccggcccag gcccggaacg aaccgacgtc gaccccgaac
     2761 cccgagagag ggttgacctg tacgatgaag agacggaaga cgaggccgag cgcgaggacg
     2821 gcgacgatcg cgccgatgcc atcccgcgtc caggttgtcc gcgtcggcgc ctccgcgtcc
     2881 cggctcggtc cgcccgacac gcggccggac gtcacgggtt ggatctcccc gaacgagcgt
     2941 tgaggtggac cgcagggtag cacagggtcg atcgccgctg gcctcggatc ccccgccgtc
     3001 gacgctgttc gtctcgatcg cggtcggagc cgccctggtg gcgatcgccc tggtcgtcta
     3061 cacgctcagt catccgtacc gcttgtataa ccacttcgag tggcaggccc tggcgttcct
     3121 cgagggccag gccgccatcc gctacccggt cgaggccacc gcgacgtcgc ccggcaacgc
     3181 cttcttccag gacgtcctcc cggtgtcgac gtcccgcggg ctcattccgt tcccgcccct
     3241 gcccgccgtg ctcctgatgc cgttcgtggc cctctggggc ctcgcggcaa atggccagct
     3301 cgtcttcgcg atcctcggcg ccgtcgatgt cgggatcgcg tggtggatgc tcggccggct
     3361 gccgatccgg acctgggtcc gggtggccac gacgctcttc ctcggcttcg ggacggtctt
     3421 ctggtacgcc gcccagatcg ggacgacctg gtatcaggca cacgtcctcg ccgtcgggct
     3481 ggccctggcc gcgatcggcg tcgcgatcgg cgccgaccgg gacgccgcgc gaagtgagga
     3541 cgacctcgac gacgccggtg ctccggtcgg tgccgcgcgg acgaggttcc gcctcggcca
     3601 cgtcgtgccc gacgcgcgcc agttcctggc cggcctcctg ttcggtctgg cctgcacctc
     3661 gcgcctgacc gtggccttcg ccgcgccttt cttcgtcctc gtcggctcgg gcggctcgtg
     3721 gcagcggcgc ggctggtcgg cggcactcgg ggcggggatc ccgatcggcc tgctcatcgt
     3781 ctacaacgtc gtgtcgaccg gccacgtctt caaccccggc taccagtacc tctacgagct
     3841 cgaggccggc ttctataccc agctcaacta tcacatcgac tggcagatcg aggatcttcg
     3901 ctacctgccc cagaatttcg ggatcatgtt cctcaatgga ccggtctggc tgcccaccgt
     3961 ggtcccgtcc gcgctggacc tgggcgggcc gctctgcacc gatcctgcgg ccgttcgcgg
     4021 gttgttcaac ccggactgtc ccctcgtcct gcccaaggac acggggatga gcatcctcct
     4081 gaccagcccg gcctacctgc tggcgctgcc ggcccttcgc tgggggttcg ggctcagtcg
     4141 cctcgtgacc gctgccagcc ttgccgtcct gatcgtggca ttcgtgaacc tgctgcactt
     4201 cagccagggc tgggtccagt tcgggtatcg cttcagcctc gacttcgcac catgggcgat
     4261 cctcctcgtc gcggtcgggc tggagcggat cgccgcccgg acaggtcccc gggacgtggt
     4321 cggtccgtcg tggcccgggg ctggcgcgat ccgcggcggg gcgaccgcgc cgctcggccg
     4381 cgccggcgcg ggggcggcct accgggtggg tgcccgggcc ctcgtcgcgc tggcggtcgc
     4441 cctcgtcgtc gggtcggtcc tcgtcaactt ctggggcgtc gtctggggcg acgtgctcgg
     4501 atggtgacgc ggatcgcgcc gccggacgcg gagggccagg agccggacgc cgtccgccag
     4561 gggccggacc ctgccctgct tgcggcgtgg atcgcagcga tcgcgatcgg cgtgatcgcc
     4621 ctcgggctct cgatctggcg cctgatgccg ggtgtcggct tctgggacac cgccgagttc
     4681 cagaccgtcc tgccggtgat gggcaccgcc cacccgaccg gttacccgag ctacgtcctg
     4741 atcggctggc tcgcgtccgt cgtcctccag ccgctgggcg aggcggccct ccgggtgaac
     4801 gtcctctcgg cgatcctcgt cggggtcggc gctgccgtga ccgtcgacct cgcccgacgg
     4861 ctgagcggct cgctggtgtt cgggatcgcg gccggtctcg ggatcgccct cacgccgatc
     4921 gtctgggcga tcggcaccca tgccgatccg cactcgctcc atttcgctct cgtcgcgatc
     4981 atcctctggc tgctcgtccg ctgggagcac gctcgccggg gcaacccgct cgagcctgcg
     5041 gcggcgcccg ctgcgtcgga tgcgtcggat gcgtcggacg cgtcggacgg tcggggcggg
     5101 gccggcagtt ccgctccaac acggaacgcc gatcgatggc tgctggctgc cgccgtggtc
     5161 gtgggcgtgt cgatcggcaa tcattcgctg accctgctgc tcgggccgcc gatcgtcctc
     5221 ttcgtcctgg cggtcgagcc gggaatcctg caacggcgac gcttcatcct cgccaacatc
     5281 gcggcgtttg ccatcccgac cgtcctcatc cggttcgaga tggtcctccg cgccggctgg
     5341 ttccgggcgc cgttcgtcta cgccgatccg agcaccttca ccgggttctg gtacgtcact
     5401 ctcggggccc agttccacgg ttggctgacc gacccgttca gcaatgcgcc aaaacggatc
     5461 accgacctcg tcgccatcgc cgtgggacag ttcgggccgc tggccgcgat cctcctcgtg
     5521 gccctcgccg tgaccgccat ccgccagcct cgctacgcgc tgctgagcgg catgaccatg
     5581 gtcctgacct gcttcttcaa ctccgtctac ccggacggcg ccatcgatcg ctattacatc
     5641 ggcccggccc tcatcgcctg gacctggctg gccatcctcg gggcgacgat cgtcgacgtg
     5701 ttcgctccgc acgactcgat ctggagctcc cggtccggcg gccggtcgac cgcctgggcg
     5761 gcgctccgcc cggtcggcct cgtcgccgtc gccggcgtgc tcctggcacc gagcctcctc
     5821 gacctgccgg gccgcgccgg gtccggggcg atcgaccagt ccggcaatcg cgcggcccag
     5881 gcctggacgg acgacgtcct ggctgcgctg gagccgaacg cggttgtcgt gtcgtggtgg
     5941 agctactcga caacgctctg gtatgccacg atcgtcgagg cacgccgcac cgacctctgc
     6001 atcatcgatg atcgcaaccg cctcgactac aacctcggag acctcgacca agtgatcgac
     6061 cgctacatcg ccacccgccc cgtgtacctg gtccggaacg ggtcgagcga gctcggcgcc
     6121 ctcgcgggcc gctataccat cgcctccctt gcgacaccgc acggcgacaa cgtcctgcag
     6181 gtcagcgcga ccgcggacca cggcgcgccg gtcgcgccgc cggtcggctc gagcgagccg
     6241 tgccgatgag cgagcgcctc gccaccctgt cgtacttctt cccggcccac aacgaggagg
     6301 cgaaccttga gggcctggtg gccgaggcac tggcgaccct cccgagcctc gccgacgcgt
     6361 tcgagatcgt catcgtcaac gacggctcgc gcgacgcgac cggacgcatc gccgacggcc
     6421 tgacggccgc tcatccgggg atcgtccggg ccgtccacca cccggtgaac ctcggctacg
     6481 gcgccgccct ccggtccggc ttccgggcgg cccgtcacga ccgcgtcgcg ttcaccgacg
     6541 gcgaccgcca gttccgggtc gaggacgtcg gccggctgat cgatcggatg gcggccggcg
     6601 atcgaccgga cgtcgtcgtc ggcttccgga tcaagcgggc cgatccgctc gtccggaggc
     6661 tctacgcccg ggcctaccgg ctggcgaacc ggatcttctt cgggctcgcg gtccgcgacg
     6721 tcgactgcgc ctgcaagctc ttccgccgtg atgcgctcga gggtctcgcg gtcgcgtcgg
     6781 acggggcctt cttctcggcg gagctcctga tcaagctccg ggcggcggga cgctccatcg
     6841 ccgaggtcgg cgttccccac cacccccgga cggccggctc ggcgacgggg gcgaagccga
     6901 gcgtcgtgtt ccgggcggtc cgcgacttct ggcgcctgcg cctgcggatg tgggccaacg
     6961 gccgacaggc gctgggccgc ggtgagtcga tcctggccgc caatccggcg gatagcgcgg
     7021 cggacagcgc gacgggtggg cgcccggcct agctgtcgga gctgcgggtc cggccctcgc
     7081 cggtgttcgg atcgacgtcg agcgagttga cgaagtcgcg gaagacgtcg agccgcgggt
     7141 cgacgatctt ctcgcctgtc gactcgaggg gcgagccctc gggcaccacg tcgccgccct
     7201 cttcgtcgcc ggtccgggac gcgtcgccgc cgagggccgc ctgctcgagg accgcctggc
     7261 tggcgaagat ccggccgccg gtccggaccg ccagggcgag cgcatcggag gggcgggaat
     7321 cgatttcgaa gatcgtgccg tcccgctcga gatggagccg ggcgtggtag gtctcctcgg
     7381 agagcgtcga gatcacgacc cgctcgaccc ggacgccgag gttctcgagg gcgctggcga
     7441 agaggtcgtg ggtcaggggc cggtcagccg tcaggccctg gagcttcatc gcgatcgcgc
     7501 tcgcctccca gggtccgatc cagatcggca ggtaccgatc gtgctcggac tccttgagga
     7561 tgacgacgtg gcgactcgag agcatgtgga cgcggacgct ctcgaccacc atctcgacga
     7621 gtccgttgct ggcgctcggc tcctcgctgg ccatggccgg attatcccac gccgaccctg
     7681 tcggtcgagc ggaccgaacg cgtcccaccg ccggagccga cggcgcggac gagcaggccg
     7741 acgataaccc cgccggcgaa cccgccgatg tgggcgaaga gggcgacccc gccgctggcg
     7801 cccgacggtc ccgacgcgat gagggtgccg agcacctgga cggcgaacca gagcccgagc
     7861 aggacgatcg cgggaaggcg aatgagctgg aagaacagcc cgaagtagac gagcgacagc
     7921 acccgtgccc gggggtagag gaccagatac gcaccgagga ccgcggcgat cgccccgctc
     7981 gccccgatca ccggcaccgt cgatcccggg gcgacgacga cctgggcggc cgccgcgacg
     8041 atcccgcccg cgaggtagaa ggcgaggaag ccgacgtgcc cgaggcggtc ctcgatgttg
     8101 ttgccgaaga tccacaggaa cagcatgttg aagccgatgt ggaacggatc gccgtggagg
     8161 aacatgctgc tgaggacggc gaacgggcca aggttcagcg gatcgctccc gccgccaccg
     8221 agctgagcgg tgagtgccgc aggcaccagt ccaaaggtcc ggaacgcgcg ggccaggccg
     8281 ctctccccct cgctcgactg gaccgccagc tcccaggcga acgccgcgac gcagaccgcg
     8341 atcaggccga tcgtcaccac cggccggcga tgggtcggat tggcgtcgcg gagcgggatc
     8401 acggcgcccg cgacgccctg gccgatggct tcggcggcgc cgacgggccg cccgacgggc
     8461 gcggcgaccc cgatgccccg ggaccgggga tcggctcgtc cttgacgccc tcgagcacgg
     8521 ccgacatcac gccgtccttc gtcgcccaga agagggtgct cggtgcgtcg ggggtcgcgc
     8581 cgagcacgac gtacatgccg cggacgtcct gccaggccgg gctgccgccg gccaggcggt
     8641 attgcgagac gaacgttccc ttcgccttgt cgtaggcgac gacccggccg ctgacgcgat
     8701 cccaggcgta gatgagcccg gtctgctttt cgctcgccga ggcgatctgc gcgtacaccg
     8761 gggccggccg gaggagcgtg tcgccgcccg gcgcgaaggc cgcggtcccg ggcgcctgga
     8821 cctgccagcc ctcgctctgg cccctgacga accgggtgat cgacccgttg tcgacggcga
     8881 agatgtcgcc gtcgatcagg agctcggtga tcttcgacac gtcgcggtcg acggtcaggc
     8941 gcggctgggc gttggagggg tagccgttgc cgtcgctgcc gggcgggtag gcgaagatgt
     9001 tccggcgcga cggatcgacg atgtagaagt tgtagagcaa ggccctcttg tcacgcagga
     9061 acgtcccgac cgcggtcacg tcgtcgcccc agcccgcggc gcccacgacc ttgaccttgg
     9121 tcgtcgtccc gtggccggac tggtcggccg ggcgccagcg ccacacgacg ttcttcgtgt
     9181 cgatgatgat gaggtcgcgg gcggcggtcg tgatcagccg cgggttgccc tcgacaccgg
     9241 ccgagacgac cttcttccag cggaagacga cggtcgcctt cttcgtccgg aggtcgaccc
     9301 ggtagacgga gttggtcgcc ttgtcgagga tgtacggcac gccctgcggg ccgaggatca
     9361 tcgcctggat gtcgagcggc ggcttcgcgt tcgcggtcga aaggatcgtc gcggccgcga
     9421 ccggcacgac gtggaacagc cggtcgagcc cgccgagcgt cttcgcccgg aggggatcga
     9481 gcgtcgctgc cgggaggttg gccttcgtgg cgatcgcgag ctcgtcccac gcggccgaca
     9541 ggagctggta ggccttctgg ggatcaccgt cgacgaggtc gaccccgccg ggaccgaaga
     9601 cctggtcgag attggcgttg atcttccgga tcgcgtccgc gcccgccgcc gccgagctca
     9661 gggcctgcgg ggtgccaccg accccgccga ccgcgtacag ggcggttccc agcgcgacca
     9721 tgacgaggac gaaggcgagg atcgccatcg ccgctcgacg ctgcgtctcg cgcttcaccg
     9781 ccgccggcgt cacccgtcga taggaggtcc gccggtgggg caggatgtcc tgggcgcgcc
     9841 ggacgaggcg cgagaagagc ccgcccgctg cgctccgggc ccgggtcgcg gaagcctgga
     9901 cggccgcgac gccgccggtc acgttgtcgg ccagcgggat cggcgagcga tccggcgccc
     9961 cggcaagcgg ctccgccggg cggaccggga cgagcgtccg cgagcggtgg gtcgatgcca
    10021 cctcggtcgc ctcgaacgcg atcatcgcgt cgctgcccgt gccgtcggcg gcgaagaagc
    10081 ggtggtggag gtgctccatc gcgggctggg ggtggagcgt gaccatcgcg tccttgagct
    10141 cgtcggggcc cagccggctc atgacgttcg gggaaacaag gacgagcgag tcgccgaccg
    10201 agatctcgcc acgccagacg tccggctcga cgtcgctgtt cggcagccca cgctcgcgat
    10261 gggggtcggg cagcgtcgac aggcgggcct ggcggatcag gtaggcctcg gccgggccga
    10321 tcgtggcgac gtacagctcg ttgccgcgca cgacggcgat cccgatcccg atcgggccgt
    10381 tcgccgcggt cccgtgcaac ccgagtcgct cgcgctggtg gttgagtcgc ttgttggccg
    10441 acgcgatcgc cttttcgatg cagacccgga tcccggccga ctcgtcgtag tagtactcgc
    10501 cgcggatcgt ctccgccgcc agctgcgtcg cctcctgggc gcgcgccgac gcgatcgtcg
    10561 aggtcacgat caggtagagg tgacccttcg agcgggcaat ggcgccgatc gacggttcga
    10621 cgacgacgac cgtgtcgggc gaattcggtg agcggtccgg ctcggcgatc acgccaagct
    10681 tcatctgcag tcggacggcc aagtcagggt ctctcgtggc tgtgagcgcc ggggttggac
    10741 gatggacggc gcggaacgcc gagacggggc ccggtttcgt tcgggacgcg cgccccgaga
    10801 ttggctggca ggtccgggca gtatacgcgg cggtcccgac ggagggcgcg gttcgtggtc
    10861 aagcccgatc ggtccggatc ggaccgggcc cgcggccggg acccgggccg tcgccccgac
    10921 cggggtgacg ggcgacgatc acccgcagct cgcgcgcacc gaacccggcc ggcgtcgcgg
    10981 cgaaggccgc gagcagctcc gggccgcgca ggcggagctc ctgggcgacg atcggggcgt
    11041 cggcttcgac gacgatcgcg aaaccctcga tccggatgac gcgacaggcg ccggcggcgg
    11101 ccggcacgcg ttcggcgacg agctgctcaa acgtggccat cgcccgggac aggcggagct
    11161 cgtcctcgag cccaagcccc cgggcagcat ccggcaacag gtcgccgatc cggaccatcg
    11221 ggcgacgacg gccgctcacc ggtcctccgg gacggatccg accggagcgg cagccacggc
    11281 gggcggacgc cgtcccgggc cgttcccgga cgcctcgggc gaccggaggg cggcaccgcc
    11341 accgccgtca gggacgacct cccacgccgt tgccgcccgg accagggccg gatcgagatc
    11401 gtcgagcgtg gtcgtcgtga cgaaggcctg gggcagggcc gcgatccgcc gcacgagatg
    11461 ggagcgccga tccgggtcga gctcggagaa cacgtcgtcg aggaggagca gcggcgggcg
    11521 gccgtcctgc tcggtcagca ggtccagctc ggcgagcttg aaggcgagga tcgcggtccg
    11581 ttgctggccg cgcgaggcga acccggacat ggcgcggccg tcgaggctga agacgagatc
    11641 gtcgcgatgc ggaccgacga gcgtggagcc gttccagagt tccttgtccg cggtctcggc
    11701 gagacgtctg gccagggcat cccgcggcga ctcgcggggg agtgccggcg cattcgtcgc
    11761 gtacgagatc gccagccgtg ccgcgccggc ctcgtccgga gcgatctccg cgtgggcccg
    11821 cgccagcggg cccgcgaggt cgtcgatcag gcgcagtcgc tcggccacga tcgccccgcc
    11881 ggcgtcgagg aacgacccgt cccagaaccg gagctcggcg cgactcgcct gctcctcgcg
    11941 aatcgcccgg agcaggctgt tccgctgctg gagcgtccgg gcataggaca cgaggtcgcg
    12001 gccgtacgcc gcggatcgct gtgttgcgag ctggtcgagg gcgccacgcc ggaggccggg
    12061 tgagccgacg acgaggagca tctcctccgg ggcgaagacg acggtccgga gcaggccgac
    12121 gagaccggcc gcccggcgcg ccacgccgtt gacccggatc cgcttccggg cgcccgctgc
    12181 gccggccggc cggaccagcg cgacctcgat cgtctcgggc ccggccacgc cctcgatccg
    12241 ggcgagctcg ctgccccacc gaaccagctc ggcgtcggac gaggtccgat gcgagcgacc
    12301 ccaggccaga aggacgatgg cctcgaggag cgtggtcttg ccggccgcgt ttggcccgac
    12361 gatcagctgg ggacccggtc cgaaggcggc ctcgagcgcg gaatacgaac gaagaccctg
    12421 gatgctgatg gactcaagca cgcggagtcc tcactcgcgc tccgaggtcc gaggccgagc
    12481 tccgcgaagc catccgcccg cggagcgagc gccgtccccg accggcctcc gtcaggaggt
    12541 cgtccggagc ggcatgacga cgtggacgta gcggtcgtcg ccgatcggct tgaacacgcc
    12601 cggcgagagc ggtccgttga gctccagggc gaactgctcg gcgtcgacgt tcgtcaggac
    12661 atcggccagg aaccgggcgt tgaaggcgat ggtcgtccca tcaccctcga tcgccgcctc
    12721 gacccgaccg acgtgatcgc cgatctccgc gttcgcgctg accgtgatcg cgttgtcgcc
    12781 gtcgagcccg acgccgagct tgacgatgtt tgccgactcg tgggcgatca gggcggccgg
    12841 gcggacggcc cgcaggagct cctcccgatc gaggaccgcc cgggtcgcat ggctggccgg
    12901 caggaccgac tggtagttcg ggtactggcc atcgatcagg cgggtcacca gatcgatgcc
    12961 atcgatgtgg aacaggagct ggttgcggga gtgggcgagg acgatcgaga ccggttcatc
    13021 cgtgtccgcc agcacgcggg ccagctcatg gagggcgcgg gccgggatga cgacgctcgt
    13081 ctcctcgacc ggatcgagga tcgtgatcgt cttgaccgcg atccggtagt tgtcggccgc
    13141 cgcgagggtc agttgatcgc cctcgaactt cgcgaggacg ccggtcagga tcggccgggc
    13201 ctcatcgctc gccgccgcga acgccgtctc gtcgagggcc tggcggagga ccttctgggc
    13261 gacccgggtg atcggccgct caccagcggt ctggaccgtc gggaagtcct cggccgggat
    13321 ccccttgatg ttcgattcga agcggcccgc ccggatgtgg agtgtctcgc cgtcgccgag
    13381 ctcgaggctg atcggctcgc tccccggcaa cgagttcacg agatcggtca ggagccgagc
    13441 gggcacggac gtcgccccgt cgatctcgat cttgcccggg acccagtagg tgatcccgat
    13501 ctcgaggttg gtcgcggtca gcttcaggcc accgtcctcg gtcttgagca ggacattcgt
    13561 caggaccggc agcgagcggt tcgagacggc ccggctcacc accgacagac cgcgagcgag
    13621 gttttcctgc atgaccgaaa gcttcacgtc gagttcactc caagtgacgg aactgcggtc
    13681 cgaacgggcc gggatggggc cgcgccgacc ggtcgatgtg tcgtgggtat cttcacccta
    13741 tcttctcatc ccgtcatcac cgtcatcatg gtgtggattt ggtggacggt tcctcggcgc
    13801 cgtgcggccg aggccggtgg acgagtccgg gtccgtggtg gacggtggtc gggcgatcgg
    13861 ggcatggcgg gtggacggac cggtggacag ccggggcagg tcgcccgacc ctcctgcgcg
    13921 ttgcccggtt tcgtccacgc cggaggtgga ctggtcgacg atcccgtgcc ggggatgtcc
    13981 acaaccgacc ccggttgtcc accgattcgg gcaccttgtc cacacccgac gccacgcaaa
    14041 ccggttcagg tcggctggcg ccagaccgca ttcgtcggtc cagggcgaca gccctagtcg
    14101 gcgtagatga gctcgcggac ggctgcgatc tctcgccgca actcatcgtt gacggccgac
    14161 tcgcgggtga tcttgtcgca ggcgtggagg accgtcgaat ggtcccggcc gccgagctcg
    14221 gccccgatcc ggaggaggga cacgtcggtc tcctcgcgca tcaggaacat cgcgatctgg
    14281 cgcggcatga cgatcgcctt gtcccgcttc tggcccttga gctggtccat ctggaccccg
    14341 tagtagtcgg agaccgcctt ggcgatccgc tccggcgtga cctgtcgctt cttcgggttg
    14401 tagaggacgt tcgagaggac ggcctgggcc aattcgatcg agatcggcat cgcccccatc
    14461 gaggcgtatg cgacgatccg gttgagggcg ccttcgagct cgcggatatt gctgacgacc
    14521 ttgcgagcga cgaactcgat cacgtcggac gtgatcgcaa ccgcgccctc ctcggccttg
    14581 gcccggagga tcgcgatccg ggtctcgagg tctggtgccg tcaggtcggc gatgaggccc
    14641 cactcgaacc gcgagcggag tcgctcctcg agggtcagga tcgccttcgg cggccggtcc
    14701 gagctcagga cgatctgctt gccgtcctca tggatcgcgt tgaacgtgtg gaagaactcc
    14761 tcctgggttc gttccttgtc ggcgatgaac tggatgtcgt cgatcaggag caggtcgatc
    14821 cgtcggtagc gagcccggaa ctcgtcgatc ttgccctgct ggatcgaggt gatgaactcg
    14881 ttggtgaact tctcgctcgt cgcgtagacg atccgcttgc gcgggaacct ggccgcgacc
    14941 tggctcccga ttgcgtgcat caggtgagtc ttgccgaggc cgaccccgcc gtacaggaac
    15001 aacgggttgt aggcgtgacc gggtcgctcg gcgaccgaca ggctcgccgc atgggcgagg
    15061 cgattggccg agccgacgat aaagttggcg aacgtatagc gcgggttgag gttggcgctg
    15121 ccgccctcgc cgccgacgcg accgggctcg agccggacct ggggcgattg cgtgaccgga
    15181 cccgccggca cgtcctcggc cggcggcgcc tcgacggtcg tgatcgtgaa ctcgacctgg
    15241 acgctgtaac ccacgatccg ggcgagcgtc tggctgatca gtgaccgata gcggttctcc
    15301 agccagtcct tggcgaagcc gttcgggacg gcgatccgga agcgctgctc atcgacgtcg
    15361 atcagctgcg tatcgcgcag ccaggtctcg aagttcgcgg gcgacaacga cacttgaagc
    15421 tcgccgagcg cggcgcgcca gacctgcttg gcatccattc gatcgatgat gtcccgatcc
    15481 gtagtggttg aagggaagat tcggccgtac gggaggccga cgcgaggtcg ccgcggacga
    15541 agctgaccgg ctccggaagg gtcgtcggca cgggcggacg acgctccagg atccatcccg
    15601 cacggggaga atggggctga cccgcgagcg tcccggcgcc gaaggcccgg tgagaatagc
    15661 accgcccgga cgggggtcgc aaggcaattc ccgcataccc ccggggagta tcccatcgga
    15721 ggcgggcgtc aagcccgatc gagcggccgt ggccgcgttc gtcggccgtt tgacactcgc
    15781 gaagagcccg tcatatcatc cgccggacga cgaacgcgcc gcgcgctcac gtgggcgctg
    15841 gcgcgtttgt gtgcctttca ggggtcggat gtcgacccgg ggcccagcgg agaggatcgc
    15901 gcacagtccc gatgaagcag acctatcagc ccaagaagcg gcatcgcgcg aaggagcacg
    15961 gcttccgcgc acggatgagg acgcccggcg ggcgtcgcat cgtcgccgcc cgacgggccc
    16021 gtggtcgaaa gcgactgacg gcctgacgag cgagcgcggc ccgcatccgc cgcggctcgt
    16081 gatgctctcc cgtccgcagg agttcgcggc gctgcaggcc gggaccgtcc ggtcccaccc
    16141 cctcctcacc gcccgatttc tgcgaacgga cctcgagacg acccggttcg gcctggcaac
    16201 aggccggaag ctgggcggtg ccgtggtccg caaccgcgtc cggcgccggc tccgcgaggg
    16261 tctcagggcg atggcgccct cgttccagcc cggctgggac gtcctgatca tcgcccgccc
    16321 ggcgatcgtc gagaccgact accggaacct ggtggagacg ttgcggcgga tcctccggcg
    16381 aggaggcgtg gtgggaggtc aggcggactc gtgaagcgga tcgggatcgg cctcatccgg
    16441 ctctaccgga tcctcttcgc ctggctgccc tcgagctgcc gctacgagcc gacatgctcg
    16501 cgctatacgg aacaggcaat cgacaagtac ggcctcctcc ggggcagctg gatggggatg
    16561 cgccggatcg cgcgctgtca tccggggcac cccggcggct acgatccggt tcgctaggca
    16621 accagacgag gaccacgtga cactcccgcg cctccgttcg agcctcgtcc ggctcctccc
    16681 gctcctcgcg atcgtggccg tcgccctgct cgtcgcggcc tgcgccggcg ggccggcggg
    16741 ctctccgggc gccagcggga gtcccgcggt cacgccgacg ccgcaggcgg cgatcccgct
    16801 cgtcccgcag aacccggaca acccgttcac cctgctcgcg tggctcttca cgccgctcta
    16861 ccagacgttc ttcatcctcc tggtggtgct cgacaagctg accggcaaca tcgcgatcgc
    16921 gatcatcctg atgacgatca tcatccggat catcctgatc ccggtcttcc gccgccagac
    16981 ggtgtcgacc aagcggacgc agatgctcca gcccgaggtc aaggagatcc agcggcgctt
    17041 caagggcgac cgcgtcaagc agcaggaggc ccagcgtcag ctctacgccg agcgcggcat
    17101 caatcccgcc tcgggctgcc tgcccgcgat cctccagatc ttcctgctga tcccgatgta
    17161 ccaggtcttc agccagggcc tccagaactt caacccgcag gcgatgctgg acgtcttcgg
    17221 cttccggatc atcgacctcc actgtgcggc cgcgccgatt gtcaccgccc tcgggcacgt
    17281 gaccaacccc tgcctggacc cgacggcctt cagcgtcaac tggggcgtcc ccgaggtgat
    17341 catcgggacg accggctcga tgctctcggg cctgagcctc ctcgcggtga tctcgtcgtt
    17401 gttccagctc gtccaatcgc ggatgacgct gccgccgcac gacccggcga tggccgacga
    17461 tccgcagtac aagatccagc gccagatggc ctacatcttc ccgttcatct cgctgatcta
    17521 cggcagcttc ctgccggccg gcctgttcct ctactggatc acctccacca tcttctcgat
    17581 cttccagcag tacctgatca tcggctgggg cgggatgttc ccgctgttcg gctggcatcc
    17641 ggggttcgcc caggggcacg cgccccggtt cccggtcgtg atgccgcccc cggtcgaccc
    17701 caagacccga ccgccgacct cgccgctggg cggggcatcc ggccgagcag cccaggctga
    17761 caagaccatt cgccacaagg aacgcggccg tcagggccgc cgagggagac gccgctgatg
    17821 agtgcctacc gagaattcac gggcaagagc gtggaagagg cgctcaaggt cgcccgcgag
    17881 gagttcggtg tcgagctgaa cgacctcgac ttcgagatcc tgaccgccgg cagccgcggc
    17941 gtcctcggga tgggtgcgga gcccgcccgg atcgttgccg cgccccgttc cgctcttggc
    18001 ggcgcggcgc ccaagcgtga ggcggccgcc accaccccgc tcccgccggc agcgccgcgc
    18061 gaggatcgcg ccttcggcga cggggatcgg gatcgcggcc cccggcgcga cgatcgtggc
    18121 ggggatcgcg gcggcttccg cggcggcgac cgtgaccgtg gcccccgacg agacgagcgg
    18181 ccggccggcg gcggcggcga tcgcgaccgc ggcccgcggc gcgacgaccg cgccttcggt
    18241 gatcgcggcc cgcgcaatga tccagcgccg cgcgatggga cacccgtcgc cgaggccgac
    18301 gccccgatcg aggccagcgc ccccaccgag gagcgcccag cgcgtccgga tcgggcggcg
    18361 ggcggcggcg atcgcgatcg tggcccccgt cgagaagacc gcgcgccgcg cggcgaccgg
    18421 cccgaccggg cgccacgaga aggtggtccc gggacctcgg ccctgaacgc gaccgaggcc
    18481 gccgaggtca aggccgcacg cggttcgctt gccgccgaga gggccgatct cgaggccgcc
    18541 gaggcatccc ctgaggcgct cgctgccggc aaggagatcc tcgaggagct gatgcgccat
    18601 atcggcttcg cggtcaaggt cgaggtcgag accggcgata cgagccgcct caacgtcgtc
    18661 gccgatcccg acggccgtga agccctcggc tcgctgatcg gccggaaggg cgagcgtctg
    18721 tccgccctgc agcacctcgt caacctgatg ctgtcgcggc ggatgggcga gtggacccgc
    18781 gtcctggtcg acgtcgagga ctaccgcggc cggcgtgagc ggcagctgcg cgacctcgcc
    18841 acccgggccg ccgcccgggt cgaggagacc ggcaagatga tccagctcga gccgatgccg
    18901 gccctcgagc ggcgctggat ccacctcgcc ctgcgcgatc accctcaggt cgccacccag
    18961 tcgatcggcg aggagccgaa ccgtcggatc gtcgtcctgt tgcgaggcgg ttgaaccgcg
    19021 ccggcctcgt ggtgcgtcac gcccagcggc tgccgggcgt catgatgccg ctcaccatcg
    19081 gattcccccc cgaccccgcc gctaggatga cccgcgaccc atgaatgccg cagccgatcc
    19141 aaccgccggc cgaccatgca tgagcctctg atgaccgacc tgcccaactc cgcagccgac
    19201 cgggctgcca aagcctccac acccgacatc gagacgcccg ccggcgaccc cgagctcgcg
    19261 gcgctcgtcc ggcgcgaacc gcgggacgac ggcgacgacc cctcgccggc cgccctggtg
    19321 aacctcgggc ggcggcccgt gtcctcggcg gtccccgccg ccccggccgc cggtgccccg
    19381 gagcggccca tcgctggggc gcctgccgcc gacgagac
//
LOCUS       DATAIW010000396         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_175828, whole genome shotgun sequence.
ACCESSION   DATAIW010000396 DATAIW010000000
VERSION     DATAIW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175828"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VHM48_11860"
     CDS             <1..767
                     /locus_tag="VHM48_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756155.1"
                     /translation="ARPPSPYWPGSDSSRIRPTDVRGCLFVLVLGAAILGGAAWFGSP
                     ILASTVIATALQNAGYHAATSTVTATSDPPPKLLLGRADQVEIRGTDVDFRTFHAASL
                     DLVLTDVDVVGRTAARISGRIVGAELATTDGTATTADVDIDGAAGAADASIVVDAATV
                     DRVVKATMKQKFGITVSATELIAPDTLRITAVGATVTGRLVVDASGAITLSTPLGSSP
                     ILSLDPSFPLRLRAVAAVEGDLRIDATLDAEALLGG"
     gene            1097..>2119
                     /locus_tag="VHM48_11865"
     CDS             1097..>2119
                     /locus_tag="VHM48_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2756156.1"
                     /translation="MPPQASTGAPAAAPSKRRAKPTGPRRTPSRPASSSVATSAVEPD
                     LGALKAQLLATVREHYPQSDLDPIERAFDLAVDAHEGQRRATGEPYVTHPIASAQITA
                     ELGIDPIAVTAALLHDVPEDTEYGLTDIEDRFGPEVVRLVDGVTKLSRFSTHSHEQQQ
                     AENIRKMLLAMAEDIRVVLIKLADRLHNMRTLYGLPSEKQLRIARQTQEIYAPLAERL
                     GIWQIKWELEDLAFKVLEPDRFRELARLLDTRRRGREDYISRAIAELTPKLTEAGIEA
                     DLQGRPKHIYSIDKKMQRKSAEFAEIYDVYAIRILVDDVRDCYAALGTVHALWRPIPG
                     QFDDYIA"
BASE COUNT          333 a          822 c          674 g          290 t
ORIGIN      
        1 gcgccagacc gccctcgccg tactggccgg gctcggattc gtcccggatc cggccaactg
       61 acgttcgcgg ctgcctcttc gtcctcgtcc tcggcgcggc aatcctcggc ggcgccgcct
      121 ggttcggctc gccgatcctc gcctcgaccg tcatcgcgac ggcgctccag aacgccggct
      181 atcacgcggc gacctcgact gtgacagcca cgtcggaccc gccgcccaag ctcctcctcg
      241 gccgtgccga ccaggtcgag atccgcggta ccgacgtcga cttccggacg ttccatgcgg
      301 ccagcctcga cctcgtcctg accgacgtcg acgtcgtcgg ccggacggcg gcccggatca
      361 gcggccggat cgtgggcgcc gagctggcca cgacggacgg gaccgcaacg accgcggatg
      421 tcgacatcga cggcgccgcc ggcgcggcgg acgcctcgat cgtcgtcgat gccgcgaccg
      481 tcgatcgggt cgtcaaggcg accatgaagc agaagttcgg catcacggtc tccgcgaccg
      541 agctgatcgc gcccgacacg ctccggatca ccgcggtcgg agcgaccgtc accggccgcc
      601 tcgtcgtcga tgcgagcggc gccatcacgc tctccacgcc gctcggttcg tcgccgatcc
      661 tgagcctgga tccgtcgttc cccctgcggc tgcgggccgt cgcggccgtc gagggcgacc
      721 tccggatcga cgccacgctc gacgcggaag cactgctcgg cggctgagga tcggcgcaat
      781 ccggggtgat cccgcctcgg atcagtccag ggcgaacgag cgtggtcgga caccggtttg
      841 cgctcgtcgg ggtcggatcg tgcggctcga ccatggtcat tcggcccaga gtgatctccc
      901 ccgggatcga gacgcctggt cggacgggct gaatcgcgca gacccgctgc tatccttgcc
      961 cgcgatgggc ccgccacgca cgacacccga ccccacgcac gcgtcacgac ggccgcccgt
     1021 cgatcatccg acggcgggtc atcccgccac gacggccaag ccacccaccg ctgctcccgc
     1081 cccgaccgac ggcccggtgc caccgcaggc gtcgaccggc gctccagcgg ccgcgccgtc
     1141 gaagcgccgc gccaagccga ccgggccacg ccggaccccg agccggccgg catcttcgtc
     1201 cgttgccact tcggcggtcg aaccggatct cggcgcgctc aaggcgcagc tcctcgcgac
     1261 cgtccgggag cactacccgc agtccgacct cgatccgatc gagcgcgcgt tcgatctcgc
     1321 cgtcgacgct catgaaggtc agcggcgggc gaccggcgag ccgtacgtga cccacccgat
     1381 cgcctcggcc cagatcaccg ccgagctcgg gatcgacccg atcgcagtga ccgcggccct
     1441 cctccacgac gtcccggagg acaccgagta cggcctgacc gacatcgagg atcggttcgg
     1501 acccgaggtc gtgcggctcg tcgacggggt caccaagctc tcccggttca gcacccacag
     1561 ccacgagcag cagcaggccg agaacatccg gaagatgctc ctggcgatgg ccgaggacat
     1621 ccgggtcgtc ctcatcaagc tggccgaccg cctccacaac atgcggaccc tgtacgggct
     1681 gccgtcggag aaacagctcc ggatcgcccg ccagacccag gagatctacg ccccgctggc
     1741 cgagcggctc gggatctggc agatcaagtg ggagctcgag gacctcgcct tcaaggtcct
     1801 cgagcccgac cgcttccgcg agctggcgcg cctgctcgac acccggcgga ggggtcgcga
     1861 ggactacatc agccgggcga tcgccgagct gacccccaag ctcacagaag ccggcatcga
     1921 ggccgacctc cagggtcgcc cgaagcacat ctacagcatc gacaagaaga tgcagcgcaa
     1981 atcggccgag ttcgccgaga tctacgacgt ctacgcgatc cggatcctcg tcgacgacgt
     2041 ccgcgactgc tacgccgcgc tcggcaccgt ccacgccctg tggcgcccga tccccggcca
     2101 gttcgacgac tacatcgcg
//
LOCUS       DATAIW010000397         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_86753, whole genome shotgun sequence.
ACCESSION   DATAIW010000397 DATAIW010000000
VERSION     DATAIW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86753"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHM48_11870"
     CDS             <1..245
                     /locus_tag="VHM48_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756157.1"
                     /translation="LEAAVRTMFAYRPAEVLAGVSAPIVAVRRITRGDESTIVSVDAA
                     RTLHPPGGLTVLHLMAPGHNLPRYRPDEVTAAILGR"
     gene            307..1419
                     /locus_tag="VHM48_11875"
     CDS             307..1419
                     /locus_tag="VHM48_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX2756158.1"
                     /translation="MRVVYSPAHLAHDVVTETVMGAVIPANEVAERAERIRTTLVADG
                     GFSIEPPTEHGTEPILAVHDPGLLRFVEEAWREARAQSIDRPNLIADTYPSFRMFEGM
                     SPEFVAGRAETRHAAGRAGWWGLDTANPLVAGTAGAARAAVDVALTAADLVLAGERAA
                     YGLCRPPGHHAARSMAGGYCFFNNAAIAAEAVARATGERVAILDVDYHHGNGTQQIFW
                     RRGDVLYVSLHADPERQYPFFLGRADETGEGEGSGANVNIPLPAGTDDETYLVALDRG
                     LAAIADTPGDVIVVSLGFDTYGHDPIGDFGLTTAVYHECGRRTAVLGRRLVILQEGGY
                     HRPSLGANARAWLRGAEGRPYEPLPAAGFGELGSDR"
     gene            1672..2361
                     /gene="folE"
                     /locus_tag="VHM48_11880"
     CDS             1672..2361
                     /gene="folE"
                     /locus_tag="VHM48_11880"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2756159.1"
                     /translation="MPDRIELPSHIRAIVDEIETEIGEEPRRAQELIEGVGAHAGNGR
                     VEAAVYEILTEIGEDPDRQGLQGTPERVHRMYTELTAGYHVDPERLINGAIFDVAYSE
                     MVVVKDIPFYSLCEHHLLPFFGQAAVAYIPRGRVIGLSKIPRVVEMYSRRLQVQERLT
                     QQVADFLQERLEPQGVGVVMEATHLCAVMRGVRKPGTIMTTSAVLGLFRTRDRTRAEF
                     FAHLDRRAPGA"
     gene            complement(2708..>2912)
                     /locus_tag="VHM48_11885"
     CDS             complement(2708..>2912)
                     /locus_tag="VHM48_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756160.1"
                     /translation="VRLERPRGVAVRLKVVGSTASATVDGRKLGSKGGESTVETAGWE
                     TSRDRVTLEVIGGSKSIEIVDRP"
BASE COUNT          413 a         1051 c         1026 g          422 t
ORIGIN      
        1 ccctcgaggc cgctgtccgg acgatgttcg cctaccgccc agcggaggtc ctcgccggcg
       61 tgagcgcgcc gatcgtggcc gtccgccgga tcacgcgggg tgacgagtcc acgatcgtgt
      121 cggttgatgc cgcccgaacc ctgcacccgc ccggcggtct gaccgtcctc cacctcatgg
      181 cgccgggtca caacctgccg cgctaccggc ccgacgaggt gacggccgcc atcctcggcc
      241 gctaggcttc gtggcgcgcc ggcgcagccc ggtctggacg atggatctgt cgcggaggac
      301 gccggcgtgc gtgtcgtcta cagcccggcc catctcgccc acgacgtcgt gaccgagacg
      361 gtgatgggcg ccgtgatccc ggcgaacgag gtcgccgagc gggctgaacg aatccggacg
      421 acgctcgtcg ccgacggcgg cttctccatc gagccgccga ccgagcacgg cacggaaccg
      481 atcctcgccg tccacgatcc ggggctgctt cgcttcgtcg aggaggcatg gcgcgaggcg
      541 cgtgcgcagt cgatcgaccg cccgaacctg atcgcggaca cctatccgag cttccggatg
      601 ttcgaaggga tgagcccgga gttcgtcgcc ggccgggccg agacgcgcca cgccgcgggt
      661 cgggcgggct ggtgggggct cgacacggcc aacccgctcg tggccgggac cgcgggtgcc
      721 gcccgggccg cggtcgacgt cgcgctgacc gccgccgatc tcgtgctcgc cggggagcgc
      781 gcggcctacg gcctgtgccg cccgcccggt catcacgccg cgcgctcgat ggccggtggc
      841 tactgcttct tcaacaacgc cgcgatcgca gccgaggccg tggcgcgggc gaccggcgag
      901 cgggtcgcga tcctcgacgt cgactaccac cacggcaacg gcacccagca gatcttctgg
      961 cgccgcggcg acgtcctgta cgtctcgctc catgcggacc cggagcggca gtacccgttc
     1021 ttcctcggcc gggccgacga gaccggtgag ggggagggga gcggggccaa cgtcaacatc
     1081 ccgctgccgg ccggaaccga cgacgagacg tacctcgtcg ccctggatcg cggcctcgcg
     1141 gcgatcgccg acacgcccgg cgacgtcatc gtcgtctcgc tcgggttcga cacgtacgga
     1201 cacgatccga tcggcgactt cggcctgacg accgcggtct atcacgagtg cggacggcgg
     1261 acggcggtcc tcggccgacg cctcgtgatc ctccaggaag gcggctatca ccggccgtcg
     1321 ctgggggcca acgcgcgggc ctggctgcgc ggcgccgagg gccgaccgta cgagccgctg
     1381 ccggccgccg gcttcggaga gctgggctcg gatcggtagc gggtgatcct cgggcgccga
     1441 ataccaacgg gatcgatatc tggcgggttg gcgagcccgg aggcccccga actcacaacc
     1501 agtccgcatg ccgagcacga atccccgagc gcgtgcccgg tgacgtcgag gtcattggca
     1561 tctgaccccc tgtcagcccc gggaatagcg atcccgtcga tattcgactg cgcagcgagg
     1621 cgctcagctc gttgactggg agattcggcc gagcgtcgta ccatcgccgt catgcctgac
     1681 cggatcgagt tgcccagcca catccgtgcg atcgtcgacg agatcgagac cgagatcggc
     1741 gaggagcctc gccgcgcgca ggagctgatc gagggtgtcg gagcccatgc cgggaacggc
     1801 cgggtcgagg ccgccgtgta cgagatcctg accgagatcg gcgaggaccc cgatcgccag
     1861 ggtctccaag ggacgccgga gcgggtccac cggatgtaca cggagctgac cgccggctac
     1921 cacgtcgacc cggagcggct gatcaacggc gcgatcttcg acgtcgccta ttccgagatg
     1981 gtggtcgtca aggacatccc gttctactcg ttgtgcgagc accacctcct gccgttcttc
     2041 ggccaggcgg cggtggccta cattccgcgc ggccgggtga tcggcctgtc caagatcccg
     2101 cgcgtcgtcg agatgtactc gcggcggctc caggtccagg agcggctgac ccagcaggtc
     2161 gccgacttcc tccaggagcg gctcgagccg cagggcgtcg gcgtggtcat ggaggcgact
     2221 cacctctgtg cggtgatgcg cggcgtgcgc aagccgggga cgatcatgac gacctcggcc
     2281 gtgctcgggc tcttccggac ccgcgaccgg acccgcgccg agttcttcgc ccacctcgac
     2341 cggcgggcgc ccggcgcata gcgacgccgt ctcgcgcgca agggcgaccg ccctgaccca
     2401 gtggttgagt cagatcaagc agctgcacgc acggccgagt cacgacggcg cccgacctcc
     2461 tcgtgctgcg gcccgatccg acccacgagg tgagtccgtg cgcgcctgac gcccgggctg
     2521 aacgcccagc gtgcggtgcg tccctcgagg ttcggcgcgt ggccgcgacg acgccgttcc
     2581 ggcgtgggat tgcacgcccg gcgttcgagg cgactgctgg gcggggtccg aggcgcggac
     2641 cgcgacggtc cgggggacgg taccgcacgc cgacctttag gcgacccgcc gcgcggtcac
     2701 tcggactcta cggtcggtcg acgatctcga tcgacttcga gccgccaatg acttcgagcg
     2761 tgacccggtc gcgactcgtc tcccaaccgg ccgtctcgac ggttgactcg ccgcccttgc
     2821 tgccgagctt ccgcccgtcg accgtggccg aggcggtcga gccgacgacc ttcaggcgga
     2881 cggcgacgcc gcgcggtcgt tcgaggcgga cc
//
LOCUS       DATAIW010000398         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_220250, whole genome shotgun sequence.
ACCESSION   DATAIW010000398 DATAIW010000000
VERSION     DATAIW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220250"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHM48_11890"
     CDS             <1..529
                     /locus_tag="VHM48_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756161.1"
                     /translation="YEADLGLSAYDAAVIVGDGAATALFEAARAADPAADPKTVANWV
                     TGAFLGLLRTDPGAAARIDPAQLADLVGRVTAGELSGTNAKEVFAAHAADGASVASIV
                     ETRGFRQISDTSALAVVVDEVIAANPKALDDYRAGKPVLGFFVGQVMKATRGQANAAL
                     VQEAVRARLEAGPDA"
     gene            526..795
                     /locus_tag="VHM48_11895"
     CDS             526..795
                     /locus_tag="VHM48_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756162.1"
                     /translation="MILNIVLWVAGIALMALGYRRGKGPWSRYQALKEQDANAARYNA
                     WRGGIRDDGTTGASIAMAILRRQALIAAGIGFAGFVLVFLGFLVK"
     gene            complement(886..1443)
                     /locus_tag="VHM48_11900"
     CDS             complement(886..1443)
                     /locus_tag="VHM48_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2756163.1"
                     /translation="MTGGPGSGSDETARGPETAEERAARRRADRERAIAYLDLAEARG
                     PVPVLDDGAIGDVLRATRRIAVVGASSNEVRPSFGVFRYLLLQGYDCVPVNPAERSVL
                     GVPAFRTLREAVDATGPIDIVDVFRRSELCEPHAREAVEIGANCLWLQLGVVNWEAAR
                     IAHDGGLAVVMSRCTAIEHRRIRAR"
     gene            complement(1440..1622)
                     /locus_tag="VHM48_11905"
     CDS             complement(1440..1622)
                     /locus_tag="VHM48_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756164.1"
                     /translation="MLDLIVKAVGGFPQPAAFDFPWHRWIDRSRNPRMSSAVGIHRCL
                     GAPLAKVEFDILAVRT"
     gene            complement(1634..2701)
                     /locus_tag="VHM48_11910"
     CDS             complement(1634..2701)
                     /locus_tag="VHM48_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756165.1"
                     /translation="MDTFLYGIPVLGILFQVGGYMIAILPTSAPIILRAATPLGFGAL
                     CGVVCERSGVVNIGIEGTMLTAAFTGWVAAIAAMALLGPAAPGPLGITPALLIGLVAA
                     VLSAVAVSLLHAWLSISLRVDQIISGTIINIAAFGITGYLNNVISAASPTSAGQFGKI
                     TPPAELIHLPIVGWLFETIFNQGPITISLLITVIVLQVLLFRSRWGLRTRAVGEHPKA
                     AETVGINVIRLRYRNVLIGGVFAGLGGAFLSMELSNSFQQGGTAGRGFIALAAMIVGR
                     WTPIGALGAALLFSLFLTLGQTIGFAPPPGQLGEIVAALPKQTFSALPYLVTIIILAG
                     LIGRSIPPAADGRPYEREATA"
     gene            complement(2701..>3426)
                     /locus_tag="VHM48_11915"
     CDS             complement(2701..>3426)
                     /locus_tag="VHM48_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756166.1"
                     /translation="AMGFVPGFLKAVSGAHEVVTTIMLNYVALNILAAAVSGPLRVPK
                     APAPVTFDVGNAALPIFLGKSGHVGILIAAVMGFVVWWILYRTTLGFEIRTAGANPDA
                     ARYAGMRPRHLVMLTMTLSGGLAGMAGAGELLGVTHQTQTSFATSVGFDSIAVALLAR
                     SNPLAIIPSALLFGAMRAGAGQMQIQASTPRELVDVLQAVILLVIVALPVVVRAVGGR
                     GARALPAETQTITKSYASESVPS"
BASE COUNT          550 a         1177 c         1256 g          443 t
ORIGIN      
        1 ctacgaggcg gacctcgggc tgtccgcata cgacgcggcc gtgatcgtcg gcgacggcgc
       61 ggcgaccgcc ctgttcgagg cggcgcgcgc ggcagacccg gccgccgatc cgaagaccgt
      121 cgcgaactgg gtgaccggcg cgttcctcgg cctgctcagg acggaccccg gggcggcggc
      181 gcggatcgac ccggcccagc tggccgacct cgtcggccgg gtgacggccg gcgagctgtc
      241 ggggaccaac gcgaaggagg tcttcgccgc gcatgccgcc gacggggcga gcgtggcctc
      301 gatcgtcgaa acgcgcggct tccgccagat ctccgacacg tcggccctgg ccgtcgtcgt
      361 cgatgaggtg atcgcggcca acccgaaggc gctcgacgat taccgtgccg gcaagccggt
      421 cctcggcttc ttcgtcggtc aggtcatgaa ggcgacccgc ggccaggcca atgcggcgct
      481 ggtccaggag gcggtccgcg cacgcctcga ggcggggccc gacgcatgat cctcaacatc
      541 gtcctgtggg tcgccggaat cgcgctgatg gcgctcggct accggcgcgg gaagggcccg
      601 tggtcgcgct accaggcgct caaggaacag gacgccaacg cggcccgcta caacgcctgg
      661 cgcggcggca tccgcgacga cgggacgacg ggcgcctcga tcgcgatggc gatccttcgc
      721 cgtcaggcgc tgatcgcggc cgggatcggc ttcgcgggct tcgtgctcgt cttcctcggc
      781 ttcctcgtca agtaggggag gcgtccctcg gcccgtccgg gcgcccgccg accgaacggc
      841 cggacgtccc cgccgcccgt ccgggcgccc gccgaccgaa gcggatcagc gggcccggat
      901 ccggcggtgc tcgatcgcgg tgcagcgact catcacgacc gcgagcccgc cgtcgtgggc
      961 gatccgggcc gcctcccagt tgacgacgcc gagctggagc cagaggcaat tcgccccgat
     1021 ctcgaccgcc tcgcgggcat gcggctcgca cagctcggag cggcggaaga cgtccacgat
     1081 gtcgatcggg ccggtcgcgt cgaccgcctc gcgcaaggtc cggaacgccg gcaccccgag
     1141 gaccgaacgc tcggcggggt tcaccggaac gcagtcgtag ccctggagga ggaggtagcg
     1201 gaagaccccg aacgatggtc gcacctcgtt cgacgacgcc ccgacaacgg cgatccgccg
     1261 cgtcgcccgc aggacgtcac cgatcgcgcc atcgtcgagg acggggaccg gaccgcgtgc
     1321 ttccgcgagg tccaggtagg cgatggcgcg ctcccggtcg gctcgccgcc gggcggcgcg
     1381 ctcctcggcc gtttcgggcc ctcgagcggt ctcgtccgac ccggatccag gaccgccggt
     1441 cacgtccgga ccgccaggat gtcgaactcg accttggcca gcggcgcgcc gaggcagcgg
     1501 tggatcccca ccgcggacga catgcggggg ttccgggaac ggtcgatcca ccgatgccag
     1561 gggaaatcga acgcggccgg ctgcggaaac ccgccgactg ctttgacgat gagatcgagc
     1621 acgggccgcg ccgtcaggcc gtcgcttccc gctcgtaggg ccggccgtcg gccgccggcg
     1681 ggatgctccg cccgatcaac ccggcaagga tgatgatcgt cacgaggtag gggagggcgc
     1741 tgaaggtctg cttcggcagc gccgcgacga tctcgccaag ctggccgggt ggaggagcga
     1801 agccgatcgt ctggccgagg gtcaggaaca gcgagaagag aagggccgcc ccgagcgccc
     1861 cgatcggcgt ccagcgtccg acgatcatcg ccgcgagggc aatgaatccg cgcccggccg
     1921 tgccgccctg ctggaacgaa ttcgacagct ccatcgacag gaatgccccg ccgagcccgg
     1981 cgaagacgcc tccgatcagg acgttccggt agcgcaggcg gatgacgttg atcccgacgg
     2041 tctcggccgc cttcgggtgc tcgccgacgg ccctcgtccg gaggccccag cgcgaccgga
     2101 acaggaggac ctggaggacg atcacggtga tgaggaggct gatggtgatc ggaccctggt
     2161 tgaagatcgt ctcgaacagc cagccgacga tcggcaggtg gatcagctcg gccggcgggg
     2221 tgatctttcc gaactggccg gcgctggtcg gcgatgccgc cgaaatcacg ttgttgagat
     2281 agccggtgat tccgaacgcg gcgatgttga tgatcgtgcc gctgatgatc tggtcgaccc
     2341 gcaggctgat cgagagccag gcgtggagga gcgacacggc cacggccgac aggaccgccg
     2401 ccacgaggcc gatcagaagg gccggagtga ttccgagggg tcccggtgcc gccggcccga
     2461 ggagcgccat cgcggcgatt gcggcgaccc agcccgtgaa ggctgcggtg agcatggttc
     2521 cctcgatccc gatgttgacg acgccggacc gctcgcagac gacgccgcac agggcgccga
     2581 agccgagcgg cgtcgccgcc cgcaggatga tcggggcgga tgtcggcagg atcgcgatca
     2641 tgtagccgcc gacctggaac aggatgccca ggaccggaat gccgtagagg aacgtgtcca
     2701 tcaggacggc accgactcgc tcgcatagga cttcgtgatg gtctgggtct cggccgggag
     2761 ggcccgcgcg ccgcgtccac cgacggcccg cacgacgacg ggcagggcga cgatgaccag
     2821 gaggatcacg gcctgcagga cgtccacgag ctcgcgcggc gtcgatgcct ggatctgcat
     2881 ctgaccggca ccggcccgca tcgccccgaa gaggagcgcc gacgggatga tcgcgagcgg
     2941 gttggaccga gcgaggagcg cgaccgcgat cgagtcgaat ccgaccgacg tcgcgaagct
     3001 ggtctgggtc tggtgggtca ccccgaggag ctcgcctgcg ccggccatcc cggcgagccc
     3061 gcccgacagc gtcatcgtca gcatgaccag gtggcgaggt cgcatgccgg cgtagcgcgc
     3121 cgcgtccggg ttggcgccag cggtccggat ctcgaagccg agggtcgtcc ggtagaggat
     3181 ccaccagacg acgaacccca tgaccgccgc gatcaggatc ccgacgtggc cggacttgcc
     3241 gaggaagatc ggcagggcag cgttgccgac gtcgaaggtc accggcgccg gcgccttggg
     3301 gacacgcagc ggacccgaca cggccgcggc gaggatgttc agggcgacgt agttgagcat
     3361 gatcgtcgtg acgacctcgt gggcgccgga gacggccttc aggaagcccg gcacgaagcc
     3421 catcgc
//
LOCUS       DATAIW010000399         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_190423, whole genome shotgun sequence.
ACCESSION   DATAIW010000399 DATAIW010000000
VERSION     DATAIW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190423"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VHM48_11920"
     CDS             complement(<1..258)
                     /locus_tag="VHM48_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756167.1"
                     /translation="MAVIVPTLRHERRLWNDGLTRIAGVDEVGVAPTCGAVVAAAVIM
                     KPGCHRIPGVRDSKTLSMAQRERLAPIIRARAVAVGVGAASV"
     gene            complement(526..876)
                     /gene="rplS"
                     /locus_tag="VHM48_11925"
     CDS             complement(526..876)
                     /gene="rplS"
                     /locus_tag="VHM48_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2756168.1"
                     /translation="MTVLDEVVSDQLRTDLPELASGDTVKVSAKVVEGNRERVQVFEG
                     IVMRLRGGGITRSITVRRIASGVGVERTFKIHSPRIEKIEVVRHGQVRRAQLYFLRDR
                     VGKAATLRERRTSR"
     gene            1015..1377
                     /locus_tag="VHM48_11930"
     CDS             1015..1377
                     /locus_tag="VHM48_11930"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX2756169.1"
                     /translation="MAPRSPGVDAGRTAVGYGLQLAFPDAAAWKDQAARVRKVAHPWL
                     IQNNTPFSSDIDLIVSDNAASGGTCFGDSGGPNFVGSSTVIAGVNSFVYNSQCAGFSA
                     VYRIDKADDVNWLATFFD"
BASE COUNT          230 a          532 c          553 g          227 t
ORIGIN      
        1 aaccgaggcg gcaccgaccc cgaccgcgac ggcgcgcgcc cggatgatcg gggcgagccg
       61 ctcgcgctgg gccatcgaca gtgtcttgga gtcgcggacg ccagggatcc gatggcagcc
      121 gggcttcatg atcacggcgg ccgcgacgac ggcgccacag gtcggggcca cgccgacctc
      181 gtcaacgccg gcgatccggg tcaggccatc gttccagagg cggcgctcgt ggcggagggt
      241 cgggacgatg acggccatcg atggtcaggg tagccggtcg atgagccgga cgcctccggc
      301 ccgcgccaac cgggcgatgg gtcggtcggc ttgagtcccg ggggactgaa ttcacgcgcc
      361 agcgagcgag acaccaaccc gagatcgacg gaatcgacgt ccggccaagc gtgccatacg
      421 gtcaggcgag tctcggggga ctcgcggctc gtcgaagggg cggacccggg aatcgaacga
      481 ccccggggat ggccggggtc gacggctgcg gcgcgagccg accgactagc ggctcgtccg
      541 ccgctcgcga agggtggccg ccttgccgac gcggtcgcgc aggaagtaaa gctgggcgcg
      601 acgaacctgg ccgtggcgga cgacctcgat cttctcgatc cgggggctgt ggatcttgaa
      661 cgtccgctcg acgccgacgc cgctggcgat ccggcggacc gtgatcgacc gggtgatccc
      721 accgccgcgg aggcgcatca cgattccctc gaagacctgg acccgctcgc ggttcccctc
      781 gacgaccttg gcggacacct tcacggtatc gccggatgcg agctcgggga ggtcggtacg
      841 cagctgatcg gagacgactt cgtcgagaac ggtcacttct tcgttccttg gttcgttcgt
      901 cggcagcctg aagctgcggg tgggtttcac gaggaacggc cgaacgtttc gaccggtcgc
      961 ggagtatagc atggcgaccg cgcgcgcccg gacgcccagg gaggagatgc ccgcgtggcg
     1021 ccgcggtcac caggcgtcga cgccggccgc accgccgtcg gctatggcct tcagctcgcg
     1081 ttccccgacg ccgcggcctg gaaggatcag gccgcccgcg tccggaaggt cgcccatccc
     1141 tggctgatcc agaacaacac cccgttctcg tcggacatcg acctgatcgt gtccgataac
     1201 gcggcgagcg gcgggacctg cttcggcgac agcggcggac cgaacttcgt cggctcctcg
     1261 accgtcatcg ccggggtcaa ctcgttcgtg tacaacagcc agtgcgccgg cttcagcgcg
     1321 gtctaccgca tcgacaaggc ggacgacgtc aactggctcg cgacgttctt cgactgaccc
     1381 tcgtcggtcc agtcggttcg aagccccggc tcccggccgg ggcttcgcga ttccggcggt
     1441 cctaccggcg gcgatcgtcc gtcggctcga tcaggtcggg ccgtcgccgc agggtccggg
     1501 cgcgggcctg ctcctcgcgc cagcgggcga ccgcgccgtg gt
//
LOCUS       DATAIW010000400         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_130810, whole genome shotgun sequence.
ACCESSION   DATAIW010000400 DATAIW010000000
VERSION     DATAIW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130810"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VHM48_11935"
     CDS             <1..976
                     /locus_tag="VHM48_11935"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2756170.1"
                     /translation="TTAVSTPLTVNVVNGSVGLTAPAIGAAVAGWTSLAATATPPGGE
                     ELERLSFFVDGAAIGSDDLSPYSVAWNSASVPLGSHLLSVRADFSDGSSLTSPTRSIS
                     VVSAGGPAVPGASIAALSAWYAATSVAVHWSGTPGTSAITSYDVRYRRAAWNGTFGAY
                     TAWLTGTAATGGTFAGSTGSTYCFSARAHDTGQVSAWTAETCTALPLDDRSLSLHGTW
                     GKGLSTAYYRGTYLRSTSHGAYLTRTGVVARRIALLATVCRTCGSVRVYWGSTLIKTV
                     SLYSSTTAYKRLISVALFSSVRTGTLKIVVSSYSKQVLIDGVAIRRVP"
     gene            complement(1155..1547)
                     /locus_tag="VHM48_11940"
     CDS             complement(1155..1547)
                     /locus_tag="VHM48_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX2756171.1"
                     /translation="MALLAWAVPYRTIDALRTAIAARRTAMRELCRDDAPLTFATAHA
                     AKGLEFDDVAVLGMERGRFPSARSLADAPDPARALEEERRLAYVAWTRARRSLTLVYD
                     PAAPSSFLLEAFDPDELGLEAGANGRDL"
     gene            1644..>1941
                     /locus_tag="VHM48_11945"
     CDS             1644..>1941
                     /locus_tag="VHM48_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756172.1"
                     /translation="MAVGSRRRDFVEATVDEVASATPDVLPIIARIEAVRARWAVAPA
                     DPDADPEAPRPADIAMALLGWAVPYRTIDALRTAIAARRTAMRELCRDDAPLTFA"
BASE COUNT          274 a          704 c          673 g          290 t
ORIGIN      
        1 gacgacggcg gtctccaccc cgttgaccgt caacgtcgtc aacggctcgg tcgggctgac
       61 cgccccggcg atcggcgccg ccgtcgccgg ctggaccagt ctcgccgcca ctgccacacc
      121 gcccggcggc gaggaactcg agcggctctc cttcttcgtc gatggggcgg cgatcggcag
      181 cgacgacctc tctccgtaca gcgtggcatg gaactccgcc agcgtccccc ttggcagtca
      241 tctcctgtcg gtcagggctg acttcagcga cggctcgagc ctgaccagtc cgacccgctc
      301 catctcggtc gtctcggccg gcgggccggc tgtgccaggc gcatcgatcg ccgccctgtc
      361 cgcctggtac gcagccacct cggtggccgt gcactggagc gggacgccgg ggacctcggc
      421 gatcacctcg tacgacgtcc gctatcggcg ggccgcctgg aatggcacct ttggggccta
      481 caccgcatgg ctgaccggga cggcggcgac cggcggcacc ttcgccggca gcaccggctc
      541 gacctactgc ttctcggccc gggcccatga caccggccag gtgtcagcct ggaccgccga
      601 gacctgcacg gcccttccgc tcgacgaccg ctcgctctcg ctccatggga cgtggggcaa
      661 gggcctgtcc acggcctact accgggggac ctacctgagg tcgaccagcc acggtgccta
      721 tctgacccgg accggggtcg tcgcccgacg gattgccctg ctggcaaccg tctgtcggac
      781 ctgcgggtca gtgagggtct actggggctc gaccctgatc aagaccgtca gcctgtactc
      841 ctcgacgacc gcctacaagc gactgatctc ggtggcgttg ttcagttcag ttcgcacagg
      901 gaccttgaag atcgtggtct cctcgtacag caagcaggtc ctcatcgacg gggtggcgat
      961 ccgccgcgtc ccatgaggac ggccggtatc ggttgcacgt gggccccgag tttgcaggcg
     1021 acgtagcgga ccggagcagc ggtcacccgg accgtgtcag gcaagcgatc gaagccggcc
     1081 cggcggagga cgccatatcc cgagctgctc ggcgaccgtc gccgtcgacc agccgtcaac
     1141 gacgtcttga agcatcacag gtcgcgcccg ttcgccccgg cttcgaggcc gagttcgtcg
     1201 ggatcgaagg cttccagcag gaatgacgat ggggcggccg ggtcgtatac gagcgtgagc
     1261 gaccgccggg cacgggtcca ggcgacgtag gcgagccggc gctcctcctc gagggctcgg
     1321 gccggatcgg gcgcatcggc gagtgatcga gcgctcggaa accgcccgcg ttccatcccg
     1381 agaaccgcca cgtcgtcgaa ctcgaggccc ttcgcggcat gggccgtggc gaacgtgagc
     1441 ggcgcgtcgt cgcggcagag ctcgcgcatg gccgttcggc gagcggcgat cgcggtccgc
     1501 agcgcgtcga tcgtccgata cggaaccgcc caggcgagga gtgccatcgc gatgtccgcc
     1561 ggccgcgtcg cttcggggtc cgcatccggg gcgggcgcgg ccgcccagcc ggcacggacc
     1621 gcctcgatcc gggcgaggag cgggtggccg tcgggagtcg ccgacgcgac ttcgtcgagg
     1681 caacggtcga cgaagtcgcg tcggcgactc ccgacgtcct gccgatcatc gctcggatcg
     1741 aggccgtccg cgcccgctgg gcggtcgcgc ccgccgaccc ggatgcggac cccgaagcgc
     1801 cgcggccggc ggacatcgcg atggcactcc tcggctgggc ggttccgtat cggacgatcg
     1861 acgcgctgcg gaccgcgatc gccgctcgcc gaacggccat gcgcgagctc tgccgcgacg
     1921 acgcgccgct cacgttcgcc a
//
LOCUS       DATAIW010000401         8633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_86870, whole genome shotgun sequence.
ACCESSION   DATAIW010000401 DATAIW010000000
VERSION     DATAIW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86870"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHM48_11950"
     CDS             complement(<1..389)
                     /locus_tag="VHM48_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX2756173.1"
                     /translation="MEYKDPSKRGRWIVVAGVVLALAAGGAAFYLINQAQQQAGQGDL
                     QKVNVVVAARPIAARQPVVPEDLTVRQVPADDTNTDAIAVRDPNDVIGQVLPITLFQG
                     QMITKSMLNTTGGAGGTTGFSILDPGET"
     gene            complement(468..2522)
                     /locus_tag="VHM48_11955"
     CDS             complement(468..2522)
                     /locus_tag="VHM48_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX2756174.1"
                     /translation="MRVAPRGPSRPSSHGRAPVRCRPAPAAPASPAIARLSRAIHGRR
                     GRDRRREGGQVLVIFALAMTVLFAAAGLAFDIGRFYSERRFLQNAADAAALAASNAMI
                     QGHSQAEADTIARAILAANFSKDPNGITPAMPPATPVYESGHFGDGNYLINGIKFDEC
                     DVRVAVQNAINYSFGRAVGLSSSTIRGQARVKCMANVLPIAVRRYVNSPGPATTNTTV
                     PCADNENQFLDFFSTQDTACLGTDTNSALRIEPSPGNAFDPVTPESDPSHHGPELAIL
                     GQGAQPGNAADFRGFIALDIRNFATTTSQLYYNKVTSGTNSNTLKAMEANWITTGGYP
                     GPQFPAAITPPDANDQVGIMSGNSTGIAIAAAASRFVAGDEVMVCVYPGDVMAIPDFT
                     VGSPGTVNLPTTGTTANAGSLKVSRNQAFSGLVTLSTLADTLDAANPMVLGTLVGAAP
                     ITYDPNGVNPSLGGGTSVTLEDMTTAGATPGIYALWIRGQAGAPYLTTKYTPISIQVG
                     TVNRDFAFTANTASQDVAAAGNNATFTLTLQNSPNKNTAFGGPVTLSVDSPLPAGVGA
                     ITFGSATVTPTKPGASTTLTINTGTMATGTYIFTVRATGMNGDSTSRKVTHLMQLTVR
                     VAPSSSTGSDEYVDIVGFAVMRITYMDANTISAYAITPVIADPNDSRLRRGQVAKLVP
                     WT"
     gene            complement(2522..3085)
                     /locus_tag="VHM48_11960"
     CDS             complement(2522..3085)
                     /locus_tag="VHM48_11960"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX2756175.1"
                     /translation="MRLVQQQTRRRGRSEGTERGQSLVEFSLILMPLFLILLGIIQFG
                     FIFNTYVTMTNAARDAARIGTVYVYDRTLTKAQNDLARNNSIKAQVLSSMNGLSQATP
                     RFTTGSTWTQSGTTYTNGDLVITYAIPDGSDGTPAVTDSDPRTGERITVNATYHQDLV
                     IPLISALLPKDTNGRMGLGGSVTMVIN"
     gene            3534..5915
                     /gene="uvrB"
                     /locus_tag="VHM48_11965"
     CDS             3534..5915
                     /gene="uvrB"
                     /locus_tag="VHM48_11965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX2756176.1"
                     /translation="MPDFRLVAPFEPTGDQPAAIERLVDGLQKGLKHQTLLGATGTGK
                     TFSIAKAIEAHQKPTLVLAHNKTLAAQLYAEFREFFPDNAVEYFVSYFDYYQPEAYLP
                     RSDTYIEKDSSRNDEIDRLRHAATHALFERRDVIIVASVSCIYGLGAPVDYGATVLRM
                     RQGGSYRRDTILRQLVDLQYQRNDQALQRARFRVRGDTLELQPASEETIIRIEFFGDE
                     VERITEVDPLTGELLAERKEVNVYPASHYATPADKMKLAVVAIEAEMEERVGGMEAEG
                     RVLEAARLRQRTTFDLEMMRELGFCTGIENYSRHLSGREAGSRPWTLLDYFPPDWLLV
                     VDESHMTIPQVVGMYKNDRTRKEILVDFGFRLPSALDNRPLTFEEFEGHLNQVVHMSA
                     TPGPYEHERSQRIVEQLIRPTGVVDPMIDVRPTDGQIDDLMEEIRLRTERGERALVTT
                     LTKKMAEDLTDYLRELGVKVQYLHSEVDTLERVAILRDLRLGVFDVLVGINLLREGID
                     LPEVTLVAILDADKEGFLRSSWSLIQMIGRAARNIGGRVVMYADRTTESMAAAIDETN
                     RRRAIQVAYNTEHGIEPTTIVKEIHDINERLRSVAERTVVYTSEREELAGRALTIDNT
                     KAIEKLVARMETEMRAAAKELDFERAAALRDEIQNIRLRVLEQDASLIVGRAAERAAS
                     SRGTGLVGAADRKRGARRDEAAAEHQTFEVMSVTVLPAGEEPTDTIDPDGLDAHDGDE
                     ASAANTASDWLPGIRDEHEDTGRTAAWRDRPTWDRTVTPNVRRRTGTRPSRRR"
     gene            5990..6535
                     /locus_tag="VHM48_11970"
     CDS             5990..6535
                     /locus_tag="VHM48_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756177.1"
                     /translation="MGSIQLRRVAAVAFALAISLGACGGGPGGAAANDPSGVVSAALA
                     AAQSGGIGKLADFACAAHKNDIMGAFGGDAGSLTAAGLKAEDVFGAMSMSVANVTTKE
                     VSKTDTTAVVHVTGDMTINVDKEKFKAVAKTLLAAQGLPADDATIDAMVSAMATSLSK
                     TQKLDEDVKVVNEGGKWLVCE"
     gene            complement(6698..7219)
                     /locus_tag="VHM48_11975"
     CDS             complement(6698..7219)
                     /locus_tag="VHM48_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756178.1"
                     /translation="MDPGTGQATTDELVTACYAEHAAGLERYVRSMVRDSDEAADVCQ
                     EAFVRLLLAGRAGNLPDAPGAWLRRVAHNLVISGHRHRQIGDRVLGRLADDDSSASLE
                     DNAIRHEENDALVAALGALRTDDRSALILAAQGYRGTEIASYLGRTELASRALLCRAR
                     GRLGAQLVAAGAF"
     gene            complement(7400..8167)
                     /locus_tag="VHM48_11980"
     CDS             complement(7400..8167)
                     /locus_tag="VHM48_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756179.1"
                     /translation="MTESGAPRGIEPGRAAATDPDHPGPAEPGPTGPGRSDPIRILVV
                     DDHPVYRDGLRALIERSPDLELAGEAATGGEAVALADTSLPDVILMDIRMPGMTGIEA
                     TRRILAARPTTRILILTMSEDDDSLFAAMRAGARGYLPKDSDSEDLVRAIRSVAGGDV
                     IFGESIATRLQAFFAVGNARPAAAAFPELTDREDEVLELIARGLANRNIATQLGISDK
                     TVRNHVANIFNKLQVADRSQAIIRAREAGLGRENDPT"
     gene            complement(8164..>8633)
                     /locus_tag="VHM48_11985"
     CDS             complement(8164..>8633)
                     /locus_tag="VHM48_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756180.1"
                     /translation="LGLVDALRQQLATIGGPDGPIVEVSADDIGAIPAAIEVAAYRIV
                     VEAVTNTVRHADAGHCRVELEREREALVIRVADDGHGIGSGPVGVGTRAMYERAAEVG
                     GELLVGAAPDGGTVVSASLPVGAAKRTIARGRDGGQPRTAEAVESAVESAVRS"
BASE COUNT         1310 a         2889 c         3102 g         1332 t
ORIGIN      
        1 gtctcccccg gatcgaggat cgagaacccg gtcgtcccgc ccgcgccgcc ggtcgtgttg
       61 agcatgctct tcgtgatcat ctgaccctgg aacagggtga tcggcagcac ctggccgatg
      121 acgtcattgg gatcgcgcac ggcgatcgca tccgtgttgg tatcgtcggc cgggacctgg
      181 cggaccgtca gatcctcggg gacgaccggc tggcgggccg cgatcggccg agcggccacg
      241 acgacgttga ccttctgcag gtcgccctgg ccggcctgct gctgggcctg gttgatcaag
      301 tagaacgcgg ccccgccggc ggcgagggcg agaacgacgc cggcgacgac gatccaccgc
      361 ccgcgcttgc tcgggtcctt gtattccatc tccacggtgc gttgcctccc agatcgcggg
      421 tcgccgtcgt gcgccgcccg ctcgcgttcg atgccgtgcc cgtccggcta agtccagggc
      481 accaacttcg ccacctgacc gcggcgcagt cgggagtcgt tcggatcggc gatcacgggg
      541 gtgatcgcat aggcggagat cgtgttcgcg tccatgtagg tgatccgcat gaccgcgaag
      601 ccgacgatgt cgacgtactc gtcgctgccc gtgctgctgc tcggcgcgac acggacggtg
      661 agctgcatca ggtgggtgac cttccggctc gtcgagtcac cgttcatgcc ggtcgcccgg
      721 acggtgaaga tgtacgtgcc ggtggccatc gtcccggtgt tgatcgtgag ggtcgtcgag
      781 gccccgggct tggtcggcgt gaccgtcgcc gagccgaagg tgattgcccc gacgccggcc
      841 ggcaggggac tgtcgacgct gagggtcacg gggccgccga acgcggtgtt cttgttcggc
      901 gagttctgga gcgtcagggt gaaggtggcg ttgttccccg cggccgcgac gtcctgcgac
      961 gcggtgttcg cggtgaaggc gaagtcacgg ttcaccgtcc caacctggat cgagatcggg
     1021 gtgtacttcg tcgtcaggta cggcgcgccg gcctggccgc ggatccagag cgcgtagatg
     1081 cccggggtcg cgccggccgt ggtcatgtcc tccagcgtga cgctggttcc gccgccgagg
     1141 ctggggttga ccccgttcgg gtcgtaggtg atcggggcgg cgccgaccag cgtgccgagg
     1201 accatcggat tggcggcatc gagggtgtcg gcgagggtcg agagggtgac gagcccggag
     1261 aacgcctggt tgcgggagac cttgagagag cccgcattgg cggtcgttcc ggtggtcggc
     1321 aggttgaccg tgccgggaga cccgaccgtg aagtccggga tcgccatgac gtcgcccggg
     1381 tagacgcaga ccatcacctc gtcgcccgcg acgaagcgac tggcggcggc agcgatcgcg
     1441 atgcccgtcg agttgccgct catgatgccg acctggtcgt tcgcgtcggg cggagtgatc
     1501 gcggccggga actgcgggcc cgggtagccg ccggtcgtga tccagttcgc ctccatcgcc
     1561 ttgagggtgt tggagttcgt gcccgaggtg accttgttgt agtagagctg cgatgtcgtc
     1621 gtcgcgaaat tccggatgtc gagggcgatg aagccgcgga agtccgccgc gttgccgggc
     1681 tgggcgccct ggccgaggat cgcgagctcc gggccgtggt gcgacggatc gctctcgggt
     1741 gtcaccggat cgaatgcgtt gcctgggctg ggctcgatcc ggagcgcgct gttggtatcg
     1801 gtcccgaggc aggccgtgtc ctgggtcgag aagaaatcga ggaactggtt ctcgttgtcg
     1861 gcgcacggga ccgtggtgtt cgtcgtggcc gggccagggg agttgacgta gcggcggacg
     1921 gcgatcggca ggacgttcgc catgcacttg acccgggcct ggccgcggat cgtgctcgac
     1981 gacagcccga cggcccgacc gaacgagtag ttgatcgcgt tctggacggc gacgcggacg
     2041 tcgcattcgt cgaacttgat cccgttgatc aggtagtttc cgtcgccgaa gtggccgctc
     2101 tcgtagaccg gggtcgcggg cggcatggcc ggggtgatcc cgttggggtc cttgctgaag
     2161 ttggcagcga ggatcgcccg ggcgatcgtg tcggcctcgg cctggctgtg tccctgaatc
     2221 atcgcgttgg aggcggcgag ggccgcggcg tccgcggcgt tctggaggaa gcgccgctcc
     2281 gagtagaagc ggccgatgtc gaatgcgagc ccagccgccg cgaacaggac ggtcatcgcc
     2341 agcgcgaaga tcacgaggac ctggccgccc tcgcggcgtc ggtcgcggcc gcgccggccg
     2401 tggatcgcgc gggagaggcg ggcgatggcc gggctggccg gagcggcggg cgcggggcgg
     2461 cagcggacgg gagcgcgccc gtgcgacgac gggcgcgagg gcccgcgggg ggcgacgcgc
     2521 atcagttgat gaccatcgtg acggagccgc cgagccccat ccggccgttc gtgtccttgg
     2581 gcagcagggc cgagatcagc gggatgacga ggtcctggtg ataggtcgcg ttgaccgtga
     2641 tccgctcgcc cgtccgcggg tcgctgtcgg tcaccgccgg ggtgccgtcg ctgccgtcgg
     2701 ggatcgcgta ggtgatgacg agatcaccgt tggtgtaggt cgtgcccgac tgcgtccacg
     2761 tgctgccggt cgtgaaacgc ggggtcgcct gcgagagccc gttcatcgag ctgaggacct
     2821 gggccttgat cgagttgttc cgggcgaggt cgttctgggc tttcgtcaac gtccggtcgt
     2881 agacgtagac ggtcccgatc cgggccgcgt cgcgggcggc gttggtcatc gtcacatacg
     2941 tgttgaagat gaagccgaac tggatgatcc cgagcaggat caggaagagg ggcatgagga
     3001 tcagggagaa ctcgacgagg ctctggcctc gctcggtgcc ctcactccgg ccccgtcgtc
     3061 gggtctgttg ctgaaccagg cgcacgatcg cgcagcctcc gcccaaggat cggtggacaa
     3121 ggtcgagtgt cgggccggca gcccgtcgct taaatgacca attggtcccc ccggacctga
     3181 ccctgccgta cggaacacct gtcgtccgct cgcaccgcac gcggcgggcc tgacatgcgg
     3241 gcaacgcgtc ccccgatgcc tgcgacgcat cggtttcggc gtgaggcggg cggcccgctt
     3301 gccagtcgat gcgtcggcga catccacgtc gaccgacatc gcgacaggag cccgatttcc
     3361 ccgtccgccg atgcggcagc ggcatcgtcg cgacgctgat gggctggcat ggacgtggcc
     3421 gcgcgcattc gggccgaaat cgatgcacga cggacatcgc acggagatcc ggcatccctc
     3481 gacgcgatac cgaacgcttg ttctgtccgg ccgattcgga gtattgtcag cgcgtgcccg
     3541 atttccgcct cgttgccccg ttcgagccca ccggcgacca gcccgcggcc atcgagcgcc
     3601 tcgtggatgg cctccagaag gggctgaagc accagacgct ccttggagcc acgggcacgg
     3661 gcaagacatt ctcgattgca aaagccatcg aggcccacca gaagccgacc ctcgtcctgg
     3721 cccacaacaa gacgctcgcc gcccagctct acgccgagtt ccgcgagttc ttcccggaca
     3781 acgccgtcga gtacttcgtc agctacttcg actactacca gcccgaggcg tacctgcccc
     3841 ggagcgacac ctacatcgag aaggactcgt cacggaacga cgagatcgac cggctccgcc
     3901 acgctgccac tcacgccctc ttcgagcgcc gcgacgtgat catcgtcgcc tctgtgtcct
     3961 gcatctacgg cctcggcgcc ccggtcgatt acggcgcgac cgtcctgcgg atgcgccagg
     4021 gtggctcgta ccgccgcgac acgatcctcc gccagctcgt cgacctccag taccagcgca
     4081 acgaccaggc gctccagcgc gcccgcttcc gcgttcgtgg cgacaccctc gagctccagc
     4141 cggcgtcgga ggagacgatc atccggatcg agttcttcgg ggacgaggtc gagcggatca
     4201 cggaggtcga tccgctgacc ggtgagctcc tcgccgagcg caaggaggtc aacgtctacc
     4261 cggccagcca ctatgcgacg ccggccgaca agatgaagct cgccgtcgtc gccatcgagg
     4321 ccgagatgga ggagcgcgtc ggcgggatgg aggccgaggg ccgcgtgctc gaggccgccc
     4381 gcctccgcca gcggacgacg ttcgacctcg agatgatgcg cgagctcggg ttctgcaccg
     4441 ggatcgagaa ctactcgcgc cacctgtccg ggcgcgaggc cggcagccgg ccgtggacgc
     4501 tcctcgacta cttcccgccg gactggctgc tggtcgtcga cgagtcgcac atgacgatcc
     4561 cccaggtcgt cggcatgtac aagaacgacc ggacccgcaa ggagatcctc gtcgacttcg
     4621 ggttccgcct gccgtcggcc ctcgacaacc ggccgctgac gttcgaggag ttcgagggcc
     4681 acctcaacca ggtcgtccac atgtcggcga cgcccggccc gtacgagcac gagcggagcc
     4741 agcggatcgt cgagcagctg atccgtccga ccggcgtcgt cgatccgatg atcgacgtcc
     4801 gcccgaccga cggccagatc gacgacctga tggaggagat ccggctgcgc acggagcgcg
     4861 gcgagcgggc cctcgtcacg accctgacca agaagatggc cgaggacctg accgactacc
     4921 tccgcgagct cggggtcaag gtccagtacc tccacagcga ggtcgacacg ctcgagcgcg
     4981 tggcgatcct gcgcgacctc cgcctcggcg tcttcgatgt tctcgtcggg atcaacctcc
     5041 tgcgcgaggg gatcgacctg cccgaggtca ccctggtcgc gatcctcgac gccgacaagg
     5101 aagggttcct gcgaagctcg tggtcgctga tccagatgat cggccgggcc gctcgcaaca
     5161 tcggcggacg ggtcgtgatg tacgccgacc ggacgaccga gtcgatggcc gccgcgatcg
     5221 acgagacgaa tcgtcgccgc gcgatccagg tcgcctacaa caccgagcac gggatcgagc
     5281 cgacgacgat cgtcaaggag atccacgaca tcaacgaacg gctccggtcg gtggcggagc
     5341 ggacggtcgt ctacacctcg gagcgcgaag aactggccgg gcgggcgctg acgatcgaca
     5401 atacgaaggc gatcgagaag ctcgtcgccc ggatggagac cgagatgcgg gcggccgcga
     5461 aggagctcga tttcgagcgg gccgccgcgc ttcgcgacga gatccagaac atccgcctgc
     5521 gggtcctcga acaggacgcc tcgctgatcg tcggacgggc cgccgaacgg gcggcgtcgt
     5581 cacgaggaac cggactcgtc ggcgccgcgg atcggaaacg gggcgcccgc cgcgacgagg
     5641 cggcggccga gcaccagacc ttcgaggtca tgagcgtcac cgtcctgccc gccggcgagg
     5701 agccgaccga caccatcgac cccgatgggc tggacgcgca cgacggcgac gaggcctccg
     5761 ctgcgaacac ggcgtccgac tggctgccgg gcatccgcga cgagcacgag gacaccggcc
     5821 ggaccgcggc ctggcgcgat cgaccgacct gggaccggac ggtgacgccg aacgtccggc
     5881 ggcggaccgg gacgcggccg agtcggcgtc gctgaaccgg ccggccacgg ggctccggga
     5941 cgtctacact cggcaggtcc cgatcgggac ggaaacggga ggtccaccga tgggttcgat
     6001 ccagctccgg cgcgtcgcag cggtcgcgtt cgccctcgcc atctcgctcg gcgcctgcgg
     6061 cggcggtccg ggcggcgccg ctgccaacga tccttcgggc gtcgtgtcgg ccgccctcgc
     6121 cgctgctcaa tcaggcggca tcgggaagct ggccgacttc gcctgcgcgg cccacaagaa
     6181 cgacatcatg ggagcgttcg gcggcgacgc cggctcgctg acggcggccg gactcaaggc
     6241 cgaggacgtc ttcggcgcga tgtcgatgtc ggtcgcgaac gtcacgacga aggaagtcag
     6301 caagaccgat acgacggccg tcgttcacgt gacgggcgac atgacgatca acgtcgacaa
     6361 ggagaagttc aaggcagtcg cgaagacgtt gttggcggcc cagggactgc cggccgacga
     6421 cgcgaccatc gacgcgatgg tgtcggcgat ggcgacctcg ctgtcgaaga cgcagaagct
     6481 ggatgaggac gtcaaggtcg tcaacgaagg cggcaagtgg ctcgtctgcg aatgaggatc
     6541 tgacccgacc cgtttggaac gccgggtcgc aagccgtgag ctgacccgtc gacggatctc
     6601 gggattcgtc aggaacgagg aagccgcccg gaccgccggg cggttttctc gtgtccgtgg
     6661 tcgtcgtcgc gaggaccggt tcgcgacgga gatgcgttca gaacgcgccg gcggcgacca
     6721 gctgggctcc gagccgtccg cgtgctcggc agagcagcgc ccgcgaagcc agctcggtcc
     6781 gtccgagata gctggcgatc tcggtgccgc gatagccctg cgcggcgagg atcagggcgg
     6841 agcgatcgtc cgtccgcaat gcgccgaggg ccgcgaccag cgcgtcgttc tcctcgtggc
     6901 ggatcgcgtt gtcctcgagg gatgcgctcg agtcgtcgtc ggccaggcgg ccgagaaccc
     6961 ggtcgccgat ctggcgatgg cgatgcccgc tgatcacgag gttgtgggcg actcgccgga
     7021 gccacgcccc gggagcgtcc gggaggttgc cggcacgacc ggcgaggagc agccggacga
     7081 aggcctcctg gcagacgtcg gccgcctcgt ccgaatcgcg gaccatcgag cggacgtacc
     7141 gctcgagtcc ggcggcatgc tccgcatagc aggctgtcac gagctcgtcg gtcgtcgcct
     7201 gtccggttcc gggatccacg atcgtttcca tcggtccgcc ctcttcaggc cgctccgtgg
     7261 cggcctggga ccggtatggc ggtcagggtg tcccgggcac atcgggcgcg tgtcccgaga
     7321 cctcgggcaa ctggtcgggc aaccgccgcg gaccgaaccg acgggcaagg cgagccgcgt
     7381 cgacgcagcc cggcgggcgt caggtcgggt cgttctcccg acccagcccc gcctcgcggg
     7441 cgcggatgat cgcctgggac cgatccgcga cctggagctt gttgaagatg ttggcgacgt
     7501 ggttccggac ggtcttgtcg ctgatcccga gctgggtcgc gatgttccgg ttggcgaggc
     7561 cgcgggcgat caactcgagg acctcatcct cgcgatcggt cagctcggga aaggcagcag
     7621 cggccggtcg ggcgttgccg actgcgaaga acgcctggag acgagtcgcg atcgactcgc
     7681 cgaagatcac gtcgccgccc gccaccgacc ggatcgcccg gacgaggtct tcgctgtcgg
     7741 agtccttcgg caggtagccg cgcgccccgg cccgcatcgc cgcgaagagc gagtcgtcgt
     7801 cctcggacat cgtgaggatg aggatccggg tcgtcgggcg tgccgccagg atccggcggg
     7861 tcgcctcgat gccggtcatg cccggcatcc ggatgtccat caggatgacg tcgggcaatg
     7921 acgtgtctgc gagggcgacc gcctcgccgc ccgtcgcggc ctcgcccgcg agctcgagat
     7981 ccggcgaccg ttcgatcagg gcgcgcagcc cgtcgcggta gacggggtga tcgtcgacca
     8041 ccaggatccg gatcgggtcg gaccgaccgg gcccggtcgg tcccggctcc gccgggccgg
     8101 ggtggtccgg gtcggtcgcg gcggcacggc ccggctcgat cccgcggggt gcgcccgact
     8161 cagtcacgat cggacggccg attcgacggc cgattcgaca gcctcggcgg tgcgcggctg
     8221 cccgccatcg cgaccgcggg cgatcgtccg cttggcggcg ccgaccggca gcgaggcgga
     8281 cacgaccgtt ccaccgtcgg gcgccgcgcc gacgagaagc tcgcccccga cctcggccgc
     8341 ccgctcgtac atcgcccggg tcccgacccc gaccggtccg gacccgatcc catgcccgtc
     8401 gtcggcgacc cggatgacga gcgcctctcg ctcgcgctcg agctcgaccc ggcagtgccc
     8461 ggcatcggcg tggcggaccg tgttcgtgac cgcctccacg acgatccggt aggcggcgac
     8521 ctcgatcgcc gctgggatcg caccgatatc gtccgcgctg acctcgacga tcggtccgtc
     8581 cggcccaccg atcgtcgcga gctgttggcg gagggcgtcg acgaggccga gcg
//
LOCUS       DATAIW010000402         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_86983, whole genome shotgun sequence.
ACCESSION   DATAIW010000402 DATAIW010000000
VERSION     DATAIW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86983"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2682)
                     /gene="aceE"
                     /locus_tag="VHM48_11990"
     CDS             complement(1..2682)
                     /gene="aceE"
                     /locus_tag="VHM48_11990"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX2756181.1"
                     /translation="MTFDEFKHQLPDIDPAETQEWLASFDQLVEQEGESRARFLVYKL
                     LKRARQLHVGLPPLTQTRYINTISPEQEPFFPGDEQIEHRIRRIVRWNAVAMVLRANT
                     RFAGIGGHLATYASAASLYEVGFNHFFKGKDGDTPGDQIFYQGHAAPGMYARAYLEGR
                     LSEDQLDHFRRETVPGQGLSSYPHPRLMPDFWEFPTVSMGLGPISAIYQARYNRYLQN
                     RGLLDTSGSRVWAFLGDGETDEPESLGALHVAAREGLDNLTFVVNCNLQRLDGPVRGN
                     GKIIQELEAVFRGSGWNVIKVVWAREWDELLARDVDGVLVEKMNNTLDGEFQKYSVAG
                     GAYIREHFFGPDPRLRRLVNHLTDDDLAKLRRGGHDYRKVFAAYKSATEKTGAPTVIL
                     AHTVKGWTLGPGVEARNITHQAKKLSEAELRVFRDRLELPIPDGKLKDAPYYHPGPDS
                     EEVQYLRERRKALGGPLPKRVVRSQPLPKPAPAIDAEFATGSDTAVSTTMVFTRLLRN
                     LIRDKQLGPRIVPIIPDEARTFGMDPLFKEVGIYAQLGQRYEPVDSELVLSYREAKDG
                     QVLEEGITEAGSMASLQAAATSYASHGFPVIPFYIYYSMFGFQRTGDQIWALADARGR
                     GFMMGATAGRTTLTGEGLQHDDGHSHILAQTVPNIRAYDPAFAYELAAVVREGIERMY
                     HRGEDVFYYVSLYNENYAQPKKAAGVDEGIIRGIYRFAEAPDLGPKAHRARLVGSGSI
                     LQQVIAARDLLAEKAGIAAEIYSAPSFPLLRRDALEAERWNRLHPAEKARIPYVSTVL
                     GPDGGPIVAATDWMKALPDMVSRWLPPYYVSLGTDGFGRSDTREALRTLFEIDPPHIA
                     AAALAELGRCGAMAPDKVAKAMATLDVDPAKLDPAAL"
     gene            complement(2725..3087)
                     /locus_tag="VHM48_11995"
     CDS             complement(2725..3087)
                     /locus_tag="VHM48_11995"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2756182.1"
                     /translation="MSFGTEDLARLAAAEEVEIETQAPDGPAHRAVIWIVVDGSDTFV
                     RSYLGPDARWYREAVANPAVALHVEGRRLSATAIPATDPDSIDRVSAGFRAKYAPGAS
                     TTAMTTQYFDTTLRLEPT"
BASE COUNT          476 a         1038 c         1171 g          514 t
ORIGIN      
        1 tcagagcgcc gccgggtcca gcttggccgg gtccacgtcg agcgtcgcca tcgccttggc
       61 gaccttgtcg ggtgccatcg ccccgcagcg ccccagctca gcaagcgcgg cggccgcgat
      121 gtgcggcggg tcgatctcga acagcgtccg gagcgcctcg cgcgtgtcgc tccggccgaa
      181 gccgtcggtt ccgagcgaga cgtagtacgg cggcagccag cgcgagacca tgtcgggcag
      241 ggccttcatc cagtcggtcg ccgcgacgat cggtccgcca tcgggcccga ggaccgtcga
      301 cacgtacggg atgcgcgcct tctcggcagg gtggaggcgg ttccagcgct cggcctcgag
      361 ggcgtcgcgg cggagaagcg ggaacgacgg ggccgagtag atctcggccg cgatcccggc
      421 cttctcggcg agcaggtcac gggccgcgat cacctgctgg aggatcgaac cggagccgac
      481 caaccgcgcc cgatgggcct tcggcccgag gtcgggcgcc tcggcgaagc gatagatccc
      541 gcggatgatc ccctcgtcga cgcctgcggc cttcttcggc tgagcgtagt tctcgttgta
      601 gaggctgaca tagtagaaga cgtcctcgcc gcggtggtac atccgctcga tgccctcgcg
      661 aacaaccgcg gcgagctcgt aggcgaacgc cgggtcgtac gcccggatgt tgggcaccgt
      721 ctgggcgagg atgtgggagt ggccgtcgtc gtgctggagg ccctcgccgg tgagggtggt
      781 ccggccggcg gtcgcgccca tcatgaagcc gcggccgcgg gcgtccgcca gcgcccagat
      841 ctggtcgccc gtccgctgga agccgaacat cgagtagtag atgtagaacg ggatgaccgg
      901 gaagccgtgc gaggcatacg aggtggccgc cgcctggagc gacgccatcg agcccgcctc
      961 ggtgatcccc tcctcgagga cctggccgtc cttcgcctcg cggtaggaca ggacgagctc
     1021 cgagtcgacc ggctcgtagc gctgaccgag ctgggcgtag atcccgactt ccttgaagag
     1081 cgggtccatg ccgaacgtcc gggcctcgtc ggggatgatc gggacgatcc gcggcccgag
     1141 ctgcttgtcc cggatcaggt tgcgcaggag cctggtgaag accatcgtcg tcgagaccgc
     1201 cgtatccgag cccgtcgcga actcggcatc gatcgccggg gcgggcttcg gcagcggctg
     1261 cgaccggacg acgcgcttgg gcagcgggcc gccgagggcc ttccgtcgct cgcgcaggta
     1321 ctggacctcc tccgagtccg gcccggggtg gtagtacggc gcgtccttga gcttcccgtc
     1381 ggggatcggc agctcgagcc ggtcgcggaa gacccgcagc tcggcctcgg acagcttctt
     1441 ggcctggtgg gtgatgttgc gcgcctcgac gcccggaccg agggtccagc ccttgacggt
     1501 gtgggcgagg atcaccgtcg gcgcaccggt cttctcggtc gcggacttgt aggcggcgaa
     1561 gaccttgcgg tagtcgtggc cgccgcgccg gagcttggcc aggtcatcgt cggtcagatg
     1621 gttgacgagc ctgcgcagcc ggggatccgg gccgaagaaa tgctcgcgga tgtaggcgcc
     1681 cccggcgacc gaatacttct ggaactcgcc gtcgagggtg ttgttcatct tctcgaccag
     1741 gacgccgtcg acgtcgcggg ccaggagctc gtcccattcc cgggcccaga cgaccttgat
     1801 cacgttccag cccgagccgc ggaagacggc ctcgagctcc tggatgatct tgccgttgcc
     1861 gcggaccggg ccgtcgagcc gctggaggtt gcagttgacg acgaaggtca ggttgtcgag
     1921 gccctcacgg gccgcgacgt ggagcgcgcc gagcgactcg ggctcgtcgg tctcgccgtc
     1981 gcccaggaac gcccagacgc gcgagccgct cgtgtcgagc aggccgcggt tctggaggta
     2041 gcggttgtag cgggcctggt agatcgcgct gatcgggccg aggcccatcg acacggtcgg
     2101 gaactcccag aagtccggca tcaggcgcgg atgcggatag gagctgaggc cctggccggg
     2161 caccgtctcc cgccggaaat gatcgagctg atcctcgctc agccgaccct cgaggtaggc
     2221 gcgggcgtac atcccgggcg cggcatggcc ctggtagaag atctgatcgc ccggcgtgtc
     2281 gccatccttg cccttgaaga aatggttgaa gcccacctcg tacagcgacg ccgccgaggc
     2341 gtatgtcgcg aggtgaccgc cgatcccggc aaagcgggtg ttcgcccgga ggaccatcgc
     2401 caccgcgttc caacggacga tccggcggat ccggtgctcg atctgctcgt cacccgggaa
     2461 gaagggctcc tgttccgggc tgatggtgtt gatgtagcgg gtctgggtca gcggcggcag
     2521 cccgacatgg agctggcgag cgcgcttcag gagcttgtag acgaggaagc gggcgcggct
     2581 ctccccttct tgctcgacca gctggtcgaa ggaagcaagc cactcctggg tctcggccgg
     2641 atcgatgtcc gggagctggt gcttgaactc gtcgaaggtc atcgatgcct cgcgggatgc
     2701 gggtgacggg tcggtcgcgc tggctcaggt gggctcgagg cggagggtgg tgtcgaagta
     2761 ctgggtcgtc atcgcggtcg ttgacgcgcc cggggcgtac ttggcacgga agccggccga
     2821 gacccgatca atcgagtccg ggtcggtcgc cgggatcgcg gtcgcggaca atcggcgacc
     2881 ctcgacgtgg agcgcgacgg ccgggttggc caccgcttcg cgataccagc gggcgtcggg
     2941 cccgaggtac gagcggacga aggtgtcgct gccgtcgacg acgatccaga tgacggcccg
     3001 gtgggcgggc ccgtccggcg cctgggtctc gatctcgacc tcttcggcgg cggcgagccg
     3061 cgcgaggtcc tctgttccaa aactcatccg cgccagcgta gccgatcgat cccggaacgg
     3121 cgtgccgggg cgaggcggcg aacgtgaacc gacccaattg atatgcgcct gcaacggttc
     3181 tgcaatgtcg aacccgaat
//
LOCUS       DATAIW010000403         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_176287, whole genome shotgun sequence.
ACCESSION   DATAIW010000403 DATAIW010000000
VERSION     DATAIW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176287"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VHM48_12000"
     CDS             complement(<1..719)
                     /locus_tag="VHM48_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2756183.1"
                     /translation="MRIVVLIKPVPDTASGGERLRPDDWRLDRTAVPAVVNANDEYAL
                     EAALKLTEAHGGEVIVVSMAGPGAPDTMRKAFAMGAASGTLVSDDSLAGSDILSTTRV
                     LAGVLRGMEYDLVIAGADTSDGGAGVVPPGVATLLGLPYLSYAAKIEPNPEAGTVRVN
                     RISPTGYEILEAPMPALVVATQALGEPRYPSLKGIMAARSKTLETKSVADVAVGDAAV
                     GGAVATTAVLGSEAPPPRAATR"
     gene            complement(762..>1957)
                     /locus_tag="VHM48_12005"
     CDS             complement(762..>1957)
                     /locus_tag="VHM48_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2756184.1"
                     /translation="PTPTYRLTEDQLAMRDAVRVLADDQIAPRAADIDRTAEFPWDVK
                     ELLASHDVLSLPFPEAYGGLGGDLLTVCLAVEQISRVCATSGLILAVQELASLPLLLA
                     GTDAQKERWFPDLAAGRKLIAFALTEAEAGSDVASARTRARRDGDGDDADWIIDGSKR
                     FISQGSVADLIAVFAVTADDDGNAPGTPVTPRHQRLTCFIVEKDMPGFTVERVEHKMG
                     IRGSPTAELGFRGVRVPDANRVGEVGEGFSIAMRTLDRSRPGIAAQAVGIAQGALDVA
                     AAYARERKQFGKPIAEFQMIAAMLADMDAQTEAARQLLYKAATEIDAGAPDAGRWSAM
                     CKLVAGDTAMRVTTDAVQVLGGYGYIDEFPVERMMRDAKITQLYEGTQQIQRLVIARA
                     LTSPR"
BASE COUNT          258 a          664 c          740 g          295 t
ORIGIN      
        1 cgggtcgcag cgcggggcgg cggcgcttcc gacccgagga cggcggtcgt cgcgacagcg
       61 ccgccgaccg cggcatcgcc caccgcgacg tcggcgacgg acttcgtctc gagggtcttc
      121 gagcgggcgg ccatgatgcc cttcaacgac gggtagcgcg gctcgccgag ggcctgcgtc
      181 gcgacgacga gggcgggcat cggtgcctcg aggatctcgt agccggtcgg gctgatccgg
      241 ttgacgcgga ccgtgccggc ctcggggttc ggctcgatct tcgcggcgta ggacaggtac
      301 ggcaggccga gcagcgtcgc gacccccggc ggcacgacgc cggcaccacc gtcggacgtg
      361 tcggcgcccg cgatgacgag gtcgtactcc atcccccgca ggacgccggc caggacgcgc
      421 gtggtcgaga ggatgtcgga tccggcgagg ctatcgtcgc tgacgagggt gccgctcgcc
      481 gcacccatcg cgaaggcctt gcgcatcgtg tcgggagcgc ccggcccggc catcgagacg
      541 acgatcacct cgccgccgtg ggcctcggtc agcttgaggg ccgcctcgag ggcgtactcg
      601 tcgttggcat tgacgaccgc cgggacggcc gtccggtcga gccgccaatc gtccgggcgc
      661 aggcgttcgc cgcctgaggc ggtatccggc acgggcttga tgaggacgac gattcgcacc
      721 ggcgggcggc ctccttgcgg cgtggtgggc gagccttgca gtcagcgagg ggacgtgagc
      781 gcccgggcga tgaccaatcg ctggatctgc tgggttcctt cgtagagctg ggtgatcttt
      841 gcgtcgcgca tcatgcgctc gacggggaac tcgtcgatgt agccgtagcc gccgagcacc
      901 tggacagcgt ccgtcgtcac gcgcatcgcc gtgtcgccgg cgacgagttt acacatcgcc
      961 gaccagcgtc cggcatcggg tgcgccggcg tcgatctcgg tcgccgcctt gtagaggagc
     1021 tggcgggcgg cctcggtctg ggcgtccatg tcggcgagca tcgcggcgat catctggaat
     1081 tcggcgatcg gcttgccgaa ctgcttgcgc tccctggcgt aggcggccgc gacgtcgagc
     1141 gcgccctggg cgatcccgac ggcctgggcg gcgatcccgg gccggctccg gtcgagggtc
     1201 cgcatcgcga tcgagaagcc ctcccccacc tcgccgacgc ggttcgcgtc ggggacgcgg
     1261 acgccgcgga agccgagctc cgcggtcggc gaaccgcgga tccccatctt gtgctcgacc
     1321 cgctcgacgg tgaagccggg catgtccttc tcgacgatga agcaggtcag gcgctggtgg
     1381 cgtggggtga cgggcgtgcc gggcgcgttc ccgtcgtcgt ccgccgtgac cgcgaagacg
     1441 gcgatcaggt cggcgacgct gccctggctg atgaaccgct tcgagccgtc gatgatccag
     1501 tcggcgtcgt cgccgtcccc gtcgcggcgg gcgcgcgttc gggccgacgc aacgtccgag
     1561 cccgcctcgg cctccgtgag cgcgaaggca atcagcttgc ggccggccgc gaggtccggg
     1621 aaccagcgtt ccttctgggc atcggtgccg gcgaggagga gcggcaacga cgcgagctcc
     1681 tggacggcga ggatgagccc gctcgtcgcg cacacgcggc tgatctgctc gaccgccagg
     1741 cagacggtca ggagatcgcc accgaggccg ccgtacgcct cggggaacgg gaggctcaga
     1801 acatcgtggc tcgcgagcag ctccttgacg tcccatggga actcggcggt ccggtcgatg
     1861 tcggcggctc gcggcgcgat ctggtcgtcg gccaggaccc ggacggcgtc gcgcatggcg
     1921 agctgatctt cggtcaggcg ataggtcggg gtgggac
//
LOCUS       DATAIW010000404         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_116626, whole genome shotgun sequence.
ACCESSION   DATAIW010000404 DATAIW010000000
VERSION     DATAIW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116626"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="neuC"
                     /locus_tag="VHM48_12010"
     CDS             complement(<1..904)
                     /gene="neuC"
                     /locus_tag="VHM48_12010"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX2756185.1"
                     /translation="MTDAPRTIAVLTTGRQDWGILHSTCVAIRARSDLRLRLLVGGMH
                     LSARHGSTIDLIVGDGFVPDAVLEWVGRGPDADPREDAPADAQASAALAAVGAELRAN
                     PVDALVLVGDRFETAAAALAATVNRVPIVHLHGGEQTLGAFDDALRHAITKLAHLHLV
                     SHAEHARRIVALGEDPASVHVVGAPGLDAAARADLPDRAALAADLGITLAPPVVVVTV
                     HPATLATDPSAPALVVAAAMDLVPATYVVTLPNADPGAAAVRATMMAAASGPSRVAVD
                     ALGERRYWGLLRIADAMLGNSSSAI"
     gene            complement(901..2637)
                     /locus_tag="VHM48_12015"
     CDS             complement(901..2637)
                     /locus_tag="VHM48_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX2756186.1"
                     /translation="MSGTILFLIPARGGSSRVPGKNLRTVAGIPLVGHAVRNARLAAA
                     RVPGGPHRVVCSTDDAAIAAVARAWGAEVIDRPAGLASAAATSVDVALHAIDVFAGAS
                     IRAIVLVQPTSPLTDPADLVAAVERFDANLGRSVTSVTRAHPAGWHVAREPGLDGAEG
                     SIRRIDAEDVSEVLSGAIYVVAPDDLRTGRLFVGPGSIGLPIPLERSVDIDEPEDLVV
                     AEAFAAARPIRPVQIAGRRIGSGPVFIIAEGGVNHDGRVELAHRLVDAAADAGADAVK
                     FQTFDPAALAAAGAPTAEYQRRSAAGGTDQRSMLSALALPADAWAAIRDHAHERGLVF
                     LSTPFDDASADLLDALGVPAFKVASGELTNLPFIGRLARRGRPLLVSTGMAEMVEVAQ
                     AVDAIAAAGDPPIALFHCVSSYPARPEDANLRAIATMRAAFAVPVGWSDHTLGIELPL
                     AAVAAGATLVEKHLTLDRTLPGPDHAASLEPTELRAMVAGIRTVESALGSGDKRPVEA
                     ERAVAAVARKSLHWARSLPPGSPISEADLAAMRPGTGLSPARQVDLVGRRTARAVTAG
                     QPVDPADTEGFQ"
     gene            complement(2634..>3736)
                     /locus_tag="VHM48_12020"
     CDS             complement(2634..>3736)
                     /locus_tag="VHM48_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEX2756187.1"
                     /translation="PGAHAPDALARAASGAAPALVAPPAIDPALAAWRDSLDSPIDAF
                     GIDLGPLLASEVATVASRWLPGMTGSIDRIERFIKRIKPAAIVLADEYHRQDWMAAAR
                     AAGIPVAAVQHGMIYPHHNGYIHAARPDSLRLPQRTFVFGRWERDLLREASVYRDDEV
                     IVGGSPRLDLVAPDPTARARIRAELGIADDERVVLVSGTWGGLYRRFHYPIALAALMD
                     RPLPGVHIVVKLHPGEPDEGPYRRIIEGVAAARGFTPPRITIIQSIDLYRLLAASDVH
                     LGIHSTVLTEAVVTRTPNLLADGLAGADLLGYVPAGVALSVRDGASLLAALDAAAAGV
                     LTEADAAAFTDAHFEPGSASERIAANLETWLR"
BASE COUNT          543 a         1264 c         1463 g          466 t
ORIGIN      
        1 cgatggcgct cgagctgttg ccgagcatcg cgtcggcgat cctcagcagg ccccagtagc
       61 gccgttcacc gagcgcgtcg acggcgacgc gggatgggcc ggacgctgcg gccatcatgg
      121 tcgcccggac ggcggccgct ccggggtccg cattcgggag ggtcacgacg taggtcgcgg
      181 gcacgaggtc catcgcggcg gcgacgacca gcgccggagc ggacggatcc gtggcgagcg
      241 ttgcggggtg gaccgtcacg acgacgaccg gaggggccag ggtgatcccg aggtcggccg
      301 cgagcgcggc gcggtccggg agatcggccc gagccgccgc atccagaccg ggtgccccga
      361 cgacgtggac gctggccgga tcctcgccga gggcgacgat ccggcgggcg tgctccgcgt
      421 ggctcacgag atggagatgg gccagcttgg tgatcgcatg ccggagcgcg tcgtcgaacg
      481 ccccgagcgt ctgctcgcca ccgtggagat ggacgatcgg aacgcggttc acggtggcgg
      541 cgagggcggc cgcggcggtc tcgaaccggt cgccgacaag gaccagggcg tcgaccggat
      601 tcgcccgcag ctcggcgccg acagccgcaa gcgcggcgga cgcctgggca tcggccggtg
      661 cgtcctccct cggatcggcg tcgggaccgc ggccgaccca ctcgagcacc gcgtccggaa
      721 cgaagccgtc gccgacgatg aggtcgatcg tcgatccgtg gcgagccgac aggtgcatgc
      781 ccccgacgag gaggcggagg cgaaggtcgg accgcgcccg gatcgcaacg cacgtcgagt
      841 ggaggatccc ccagtcctgg cgacccgtgg tgaggacagc gatcgttcgc ggggcgtccg
      901 tcattggaag ccctccgtgt cggcgggatc gaccggctga ccagccgtga ccgcccgagc
      961 cgttcgtcgg ccgacgagat cgacctggcg cgccggactg aggcccgtgc cggggcgcat
     1021 ggctgcgaga tcggcctcgg agatggggct tccgggcggc agcgaccgcg cccagtgcag
     1081 gctcttgcgg gcgaccgcgg cgaccgcccg ctccgcctcg acgggccgct tgtcgcccga
     1141 acccagggcg gactcgacgg tccggatgcc ggccaccatc gcccgcagct cggtgggctc
     1201 gagcgatgcc gcgtgatcgg gccccggcaa tgtccggtcg agggtcaggt gcttctcgac
     1261 gagggtcgcc ccagctgcga cggccgcgag cggcagttcg attccgagcg tgtggtccga
     1321 ccaaccgacc ggcacggcga acgcggcgcg catcgtcgcg atcgcccgga ggttggcgtc
     1381 ctccgggcga gccgggtagc tcgacacgca gtggaagagt gcgatcggcg gatcgccggc
     1441 ggcagcgatc gcgtcgacag cctgggcgac ctcgaccatc tcggccatcc cggtcgacac
     1501 gagcagcggc cggccgcgac gggcaagacg cccgatgaac ggcaggttcg tcagctctcc
     1561 cgaggcgacc ttgaaggccg gcacgccgag cgcgtcgagg aggtcggccg aggcatcgtc
     1621 gaacggcgtc gagaggaaca cgaggcctcg ttcgtgagcg tgatcgcgga tcgcggccca
     1681 ggcatcggcc ggcagggcca gcgcgctgag catcgaccgc tggtcggtac cgccggcggc
     1741 ggaccgccgc tggtattcgg cggtcggcgc gccggcggca gcgagggcgg ccggatcgaa
     1801 cgtctggaac ttgaccgcgt ccgcgccggc gtcggctgcc gcgtcgacga ggcgatgggc
     1861 gagctcgacc cgaccgtcgt ggttgacccc gccctcggcg atgatgaata ccggtcctga
     1921 accgatccgg cgtccggcga tctggactgg gcggatcggt cgggcagccg cgaatgcctc
     1981 cgcgacgacg aggtcttcgg gctcgtcgat gtcgacgctc cgctcgagcg ggatcgggag
     2041 gccgatcgat ccggggccga cgaagagacg accggttcgc agatcgtccg gggcgacgac
     2101 gtagatggcg ccgctcagga cctcggagac atcctccgcg tcgatccgcc ggatcgagcc
     2161 ctcggcaccg tccaggccgg gctcgcgagc gacgtgccag ccggcggggt gggcgcgtgt
     2221 cacgctcgtg accgaccgac ccaggttggc gtcgaagcgc tcgacggcgg ccacgagatc
     2281 agccggatcg gtgagcggtg aggtcggctg gacgaggacg atggcgcgga tcgaggcgcc
     2341 ggcgaagacg tcgatcgcgt ggagtgccac gtcgacggac gtggccgccg cgctggcgag
     2401 accggccggt cggtcgatga cctcggcgcc ccaggcccgg gccacggccg cgatggccgc
     2461 gtcgtcggtc gagcacacca cccggtgggg gccgccgggc acgcgagcgg cagcgagccg
     2521 ggcgttccgg accgcatgac cgaccagcgg gatcccggca acggtccgga ggttcttgcc
     2581 gggcacccgc gagctcccgc cgcgggccgg gatcaggaac aggatcgtgc cgctcatcga
     2641 agccacgtct cgaggttcgc ggcgatccgt tcgctcgccg atcccggctc gaagtgagcg
     2701 tccgtgaagg ccgccgcgtc ggcttccgtg agcaccccgg ctgccgccgc atccagcgcc
     2761 gcgagcaggc tggcgccgtc gcggacggag agggcgaccc ccgccgggac atagccgagc
     2821 aggtcggcgc cggcgaggcc gtcggccagc aggttcgggg tccgggtcac caccgcctcc
     2881 gtgaggaccg tcgagtggat cccgagatgg acgtcggacg ccgcgaggag ccggtacagg
     2941 tcgatcgact ggatgatcgt gatcctcggc ggggtgaacc ctcgtgcggc cgcgacgccc
     3001 tcgatgatcc ggcggtaggg accctcgtcg ggttcgccgg gatggagctt gacgacgatg
     3061 tggacgcccg gcagcggacg atccatgagg gcggccaggg cgatcgggta gtggaaccgg
     3121 cggtagagac cgccccaggt gccggacacg agcacgaccc gctcgtcgtc ggcgatgccg
     3181 agctccgccc ggatccgggc ccgcgcggtc gggtccggcg cgaccaggtc gagtcgcggc
     3241 gagccaccga cgatgacctc gtcgtcgcgg tagacgctcg cctcgcgcag gaggtcgcgc
     3301 tcccagcgcc cgaacacgaa ggtccgctgg ggcaggcgaa ggctgtcggg acgggccgcg
     3361 tggatgtagc cgttgtgatg ggggtagatc atgccgtgct ggacggccgc gaccggtatc
     3421 ccggcggcgc gggcggcggc catccaatcc tggcggtgat actcgtcggc aaggacgatg
     3481 gcggccggct tgatccgctt gatgaagcgt tcgatccgat cgatcgagcc ggtcatcccg
     3541 ggcagccagc gcgacgccac ggtggcgacc tcggaggcga gcagcgggcc gaggtcgatg
     3601 ccgaacgcgt cgatcgggct gtcgagcgaa tcgcgccagg ccgcaagcgc cggatcgatc
     3661 gctggcgggg ccacgagagc cggggcagca ccggaggctg cgcgtgccag ggcatctggg
     3721 gcatgggcgc cggggg
//
LOCUS       DATAIW010000405         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_26978, whole genome shotgun sequence.
ACCESSION   DATAIW010000405 DATAIW010000000
VERSION     DATAIW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26978"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VHM48_12025"
     CDS             complement(<1..381)
                     /locus_tag="VHM48_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2756188.1"
                     /translation="MMDERARLLDAVAAVPGRLAAAARTASPEPPAPGEWTPSDVVRH
                     LIAVEIEVWHPRLAQLETEDHPSWPWVEPDRWTAEPEASLDRLLAAYADARSTTVATL
                     GALDDAGWARTGTHATFGVLDVAGL"
     gene            571..1044
                     /locus_tag="VHM48_12030"
     CDS             571..1044
                     /locus_tag="VHM48_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2756189.1"
                     /translation="MNPSDLYRTLQVDPEAEFEVIRAAYRVLAAKHHPDVGGSAETMA
                     RINSAWTTLSDRAARAAYDHQRHLRDGAARWDAYGKAARPTSADAGTILEFGRYAGWA
                     IPDVAWTDPDYLEWLVRTPNGRRYQLEIDLALGHGPATAPQVDPMVRANKRGRHH"
     gene            complement(1052..>1831)
                     /locus_tag="VHM48_12035"
     CDS             complement(1052..>1831)
                     /locus_tag="VHM48_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2756190.1"
                     /translation="GRGRSVEEPLLDAAAATGGERPSGALAVHISTSYPTGQIHIRPG
                     PQMAATDVIRITVRGRGGHASEPHAAVDPITVAAEIVLALQAMVTRRINVFDPAVVTI
                     AQITAGTTNNIIPETAFLFGTIRTVSEATRAEVRAGVRRVAEGIAAAHAATAEVDLEP
                     GYPVTVNDPAFTAFVQDTARGLIGAERVVEMPSPRMGAEDVSYVLQQVPGAMAYLGAR
                     PDGLDPATAPHNHSNLVVFDEEAMSVGVALYAAVAIRHLGG"
BASE COUNT          250 a          655 c          678 g          248 t
ORIGIN      
        1 caggcccgcg acgtcgagga cgccgaacgt ggcatgcgtc ccggtccggg cccagccggc
       61 gtcgtcgaga gcccccagcg tggccaccgt cgtcgagcgg gcgtcggcgt acgccgcgag
      121 gagccggtcg agcgacgcct cgggctccgc ggtccagcgg tcgggctcga cccacggcca
      181 gctcggatga tcctcggtct cgagctgggc gaggcgggga tgccagacct cgatctcgac
      241 cgcgatcagg tgacggacga cgtccgacgg cgtccactcg cccggcgccg gcggttccgg
      301 agacgccgtt cgggcggcag ccgcgagccg gccggggacg gccgcgaccg cgtcgagcag
      361 ccgcgcgcgc tcgtccatca ttcgaggtcg aagatcgttg cgtcgctcat gcgcccattg
      421 tcggcgcccg gtcgctcgaa tgcgatctcg ctgatcggcg actggcgagc cccctacggc
      481 attcggcgga cccggctatg gcacgatcgc ccaccggcca tcctcatcgg agccggggtc
      541 ggcgaacccg ccttcggtca cgatgcccga atgaacccgt ccgacctcta ccgcacgctg
      601 caggtcgatc ccgaggccga gttcgaggtg atccgggcgg cctatcgggt cctcgcggcg
      661 aagcatcatc cggacgtcgg cgggtcggcc gagaccatgg ccaggatcaa ctccgcctgg
      721 acgaccctgt cggatcgggc cgcccgcgca gcctacgatc accagcgcca cctgcgcgac
      781 ggagccgccc ggtgggacgc gtacggcaag gctgcccggc cgacctcggc tgacgcgggg
      841 acgatcctcg agttcgggcg ctatgccggc tgggcgatcc ccgatgtcgc atggaccgat
      901 cccgactacc tcgaatggct cgtccggacc ccgaacggcc ggcgctacca gctcgagatc
      961 gatctggcgc tcggccatgg accagcgacc gcgccgcagg tcgacccgat ggtccgcgcc
     1021 aacaagcgcg ggcgccacca ctgatcgccg gtcagccgcc caggtggcgg atcgcgaccg
     1081 ccgcgtagag ggcgacaccg acggacatcg cctcctcgtc gaacacgacg aggttcgagt
     1141 ggttgtgcgg tgccgtggcc gggtcgaggc cgtccggccg ggcaccgagg taggccatcg
     1201 cgccgggcac ctgctggagg acgtacgaga cgtcctcggc gcccatccgc ggtgacggca
     1261 tctcgacgac ccgctcggcg ccgatcagcc cgcgcgcggt gtcctggacg aacgcggtga
     1321 acgcggggtc gttgaccgtc accgggtagc ccggctcgag gtcgacctcg gcggtcgcgg
     1381 cgtgggcggc ggcgattccc tccgcgaccc gccggacgcc ggcccggacc tccgcccggg
     1441 tggcctccga caccgtccgg atcgtgccga acaggaaggc agtctcgggg atgatgttgt
     1501 tcgtcgtgcc ggcggtaatc tgggcgatcg tgacgacggc ggggtcgaag acgttgatcc
     1561 ggcgggtcac catcgcctgg agggcgagga cgatctcggc cgcgaccgtg atcgggtcga
     1621 ccgcggcgtg gggctcggac gcgtggccgc cacggccccg gacggtgatc cggatgacgt
     1681 cggtcgcggc catctggggc ccgggccgga tgtggatctg gccggtcggg tagctcgtcg
     1741 agatatggac tgcgagggcg ccgctcgggc gctcgccgcc ggtcgcggcc gcagcgtcga
     1801 ggaggggctc ctcgacgctg cggccgcgac c
//
LOCUS       DATAIW010000406         9944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_176593, whole genome shotgun sequence.
ACCESSION   DATAIW010000406 DATAIW010000000
VERSION     DATAIW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176593"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VHM48_12040"
     CDS             <1..1007
                     /locus_tag="VHM48_12040"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2756191.1"
                     /translation="ILDADHSNMAYAVRRAKREGADRFHIDIMDGHFVPNLTFGAKMI
                     KGIRPRTELPLDAHLMISDPLQYVDEFLDAGCDSVTIHAEVDPAQIEPTLRKIRAAGR
                     APGLAVKPKTPLSALEPYRGLFDIVLVMTVEPGFGGQSFMKDVAAEKILAARQYLAHK
                     PVEGEVQVDGGVNRESAEFVGGLGVDILIVGSVLWIKGRNMGREIRLVRALADEGYQY
                     RLNDGKPPIPRDAMVMFAQLPKHLAWSFMLDIEKGGIPVIPLRGTGQFNPDGVRDYDL
                     LVPASAEALTIERHTADRERYLAAAARWREEYIAEFGIKPPETLRPMSDWSRVRPPQQ
                     "
     gene            1004..1450
                     /gene="dtd"
                     /locus_tag="VHM48_12045"
     CDS             1004..1450
                     /gene="dtd"
                     /locus_tag="VHM48_12045"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2756192.1"
                     /translation="MRALLQRTTSARVRVATEVVGEISAGLVVLLGVGPDDDDATTDA
                     LARRVTELRIFDDTAGRTNLSLVDVGGAVLVVSQFTLYADTRRGRRPGFTGAAAPELA
                     ERLYLRFAGALRDLGLTVATGRFGAVMAVELVNDGPFTIWLDTADR"
     gene            1450..2106
                     /locus_tag="VHM48_12050"
     CDS             1450..2106
                     /locus_tag="VHM48_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756193.1"
                     /translation="MGLLLFAGLATFGATVAIGIIAAGTPRPSGAAVPMPPSRRMAVI
                     LFALASGGGLWGVFVALLAAASGSKGGGASARDVAGAPLVAVLPIVGAVVALVLMMRA
                     FSRVDPWILGRSIRFIVPEVVLAIVAAILTVVLDGPAAEMEAGPFLLLGFVSTGAAIW
                     LGVLGVGSVQALAVLDGSAPDDSVVRAEIDRVFKRCVPFVIASIASAALAILLVSSRL
                     "
     gene            complement(2450..2686)
                     /locus_tag="VHM48_12055"
     CDS             complement(2450..2686)
                     /locus_tag="VHM48_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HEX2756194.1"
                     /translation="MRSHHPTDLEDNMARSCAICGKVSMGGFNPQSSGMNRVRAHRRY
                     QPNLQPFTVRDSNGHETKSLVCTRCRRTSLKAAK"
     gene            2815..3258
                     /locus_tag="VHM48_12060"
     CDS             2815..3258
                     /locus_tag="VHM48_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2756195.1"
                     /translation="MPTDPSTPTPGRSLVSRRAIVDVVRSATLGSYGVIGFAGGGWFG
                     GIAERLGLAPRGIQVQLVGGRLAIDLDLLVAHGLPIAEVARQVDSAVRYGIRRALDRE
                     LDRLTIHVDGLRIAPYDVRRPGAGRQRDALEVAPDDLAASGTDVA"
     gene            3258..5147
                     /locus_tag="VHM48_12065"
     CDS             3258..5147
                     /locus_tag="VHM48_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX2756196.1"
                     /translation="MVRQHCTGAGFLDAIRSAVANIEKHVDEINALNVFPVPDGDTGT
                     NMLATVRAALDAAEASGRDATAERVAAAVSHGALMGARGNSGVITSQILRGIAEGLAG
                     RRRFNGLDLANALALGSKTAYGAVGKPVEGTILTVIRESSAAAVEAAEQDDAMESVLA
                     ATVAAAEKSVARTPSLLPILREAGVVDAGGEGLFRLFEGALLSVRGRPVNAESSAGSG
                     RLEGEARTAVAPAFRSGVAALEEDAFGYETVFVLTVADGKTLDVRAIQSRLEGLGNSV
                     LVGGDGRLVKVHVHNERPDKIIAYGLSLGTLTRITVENLDTMADDVREARASEFVGDA
                     SAAEGSGHRSRGADSSSEAARGAAGAVAVEARPTVVPGTAPVNMDDLPKPGRNGSDPS
                     SDGRPAEGPSEDTVSVDPALGPAIVAVVAGDGLEKLFRDFGVAHILRGGQTANPSTGE
                     LLRIARLARSREVILLPNNPNVRLAAEQAARICDDRRLVVVPTRNAAEGFAALLAYDP
                     DADAATNVEPMTKAGRGLASIQVTEAVRDARIGGKRVKKGQTIALDPDDGLVAVDSDR
                     DRAVLKAVGTFSAGVELVTVYYGEDASLAETEAISKRISAQIGAEVEVVHGGQPHYRY
                     LISAE"
     gene            5152..7407
                     /locus_tag="VHM48_12070"
     CDS             5152..7407
                     /locus_tag="VHM48_12070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX2756197.1"
                     /translation="MTQTRDGFRPPPSGPRKAKAPSPPPPPTDPAELLETMLNRSGLG
                     AANVLQRAGRRLRFLTVRELLFHLPRRYDDLREMRKLGELRFIEEGTVVSARVRVVDV
                     RVEPSFRRRIQRTIAEFEDETGSIEATWFGRRYIERRLFPGADIIVSGKLKHFGRKLT
                     LDNPDFQPIGAEGEMLHVGRIVPIYRLTAGLTAARLRIAMREALDRAGVHYEEYLPAS
                     IVAEEGLEGIARALEDAHYPSTFEGRDAALLRLAFDELLALQLGMVGRRRQRGRDAAR
                     PIAIDDAADAALRGSLTAALGRKLTRAVALTVDQDTAIGAIRDDLARETPMLRLLQGD
                     VGSGKTAVAAWALAAAARNGLQGALLAPTDLLARQHHATLAALLEDAAVPVELLTGSQ
                     STAQSKRTLELVKSGQAPVVVGTHALIQDRVEFAALGVVVIDEQHRFGVEQRGQLEAK
                     AGGHAPHVLLMTATPIPRTLGQVLYADLDVSDLRTPPEGRIAIRTGIRHPDNLGSTWE
                     RVRAEAAAGSRTFVVVPLIDEAEPADDDGAANPTIFDSSAPAAEAESVRLRHLLAPLR
                     VGMVHGRMKPVDRDTEMSRFRDGELDVLVGTTVIEVGVDVAEATMMVIEGADRFGLAQ
                     LHQLRGRVGRGTTESFCVLVSDTIDETAQARLKAVAEIRDGFELAEKDFELRREGDVL
                     GLVQSGLPRLRVASLQNREHVALARHARDHAEALLDDAGRLGPDAAPLLRELERGWLA
                     RVWAGEPASGA"
     gene            7411..8010
                     /gene="rsmD"
                     /locus_tag="VHM48_12075"
     CDS             7411..8010
                     /gene="rsmD"
                     /locus_tag="VHM48_12075"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX2756198.1"
                     /translation="MADAGRVIAGSAKGIRLRAPGAGTRPLADRVKQTLFAILEPELD
                     GARVLDLFAGSGAGGIEALSRGAAAAIFVEKDQGAAAVIDANLRATGLAGPTVAVIRW
                     DVVRWLAEPNVEAAFDLVLVDPPYAETELLTRVLEILGADHAPLAPDARVVAKHFWRD
                     RPPERIGRLAHERDRRFGETALTFYRRTGSEPPASEESG"
     gene            8007..8507
                     /gene="coaD"
                     /locus_tag="VHM48_12080"
     CDS             8007..8507
                     /gene="coaD"
                     /locus_tag="VHM48_12080"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2756199.1"
                     /translation="MTVAVYPGSFDPITNGHLDVIARATAVFDRVVVAVLANPRKEPL
                     LAADERVAIIREALAAGAPGVAPEKVGVEAFDGLTVDLCHRIGAGFIVRGLRAISDFE
                     TEMLLAHNNRRLAPDVDTVFFMTSIEHSYVASSLVKEISTFGGDVSAMIPPAALAALR
                     RAIGPG"
     gene            8534..9538
                     /locus_tag="VHM48_12085"
     CDS             8534..9538
                     /locus_tag="VHM48_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756200.1"
                     /translation="MDGSGAVIETRGLVKRFGDVNAVDGIDLDVRAGGVYGFLGPNGA
                     GKTTTIRIFAGLLWPTRGSTRIFGEPVAPEAAVLARVGSLIERPALYPYLSAFENVRV
                     FGTAHGLGPDVLVQRAAESLDRVGLAAVARRHAGGFSTGMRQRLALAIALIGRPDLVI
                     LDEPTNGLDPNGVVDVRELIARLAADGTTVFLSSHVLPEVEQLCDRVAILREGRIVAE
                     GPTGDLLGTGERLYVRFDTPAEAAAALAILGANATPADAGPTHPGSALIVDAPIAEAS
                     RINRTLGAAGLYPAELAPRRESLEAVFRELTSDHPDGPPPTTPAPPPVAAAPTAGPAR
                     "
     gene            9535..>9944
                     /locus_tag="VHM48_12090"
     CDS             9535..>9944
                     /locus_tag="VHM48_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756201.1"
                     /translation="MTALLRADWLRFRRRRDFWIIAIAVALIGGIGFLASYRSEVQDP
                     APFNEAEFRQQAIDGGFFEGVPPEELQAQIDQFAADMRASDEQNRIEWDREQAINLQK
                     YDIAQSPFTIIGVGIAPLIALILAASLAVGDEFR"
BASE COUNT         1369 a         3573 c         3545 g         1457 t
ORIGIN      
        1 gcatcctcga cgccgaccac tcgaacatgg cgtacgcggt ccggcgggcc aagcgtgagg
       61 gcgccgaccg cttccacatc gacatcatgg acggccattt cgtcccgaac ctgaccttcg
      121 gggcgaagat gatcaagggc atccggccac ggacggagtt gccgctcgac gcccacctga
      181 tgatcagcga ccccctccag tatgtcgacg agttcctcga tgccggctgc gactcggtca
      241 cgatccacgc cgaggtcgac ccggcccaga tcgagccgac gctgcggaag atccgggccg
      301 ccggccgggc gccgggtctg gcggtcaagc cgaagacgcc gctgtcagcg ctcgagccat
      361 accgcggcct cttcgacatc gtcctcgtca tgaccgtcga gcccgggttc ggcggccagt
      421 cgttcatgaa ggacgtcgcg gccgagaaga tcctcgccgc ccgccagtac ctggcccaca
      481 agccggtcga gggcgaggtc caggtcgatg gcggcgtcaa tcgcgagtcg gccgagttcg
      541 tcggtgggct cggggtcgac atcctcatcg tcggctccgt gctgtggatc aagggccgca
      601 acatgggccg tgagatccgc ctcgtccggg ctctcgccga cgagggctac cagtaccgcc
      661 tcaacgacgg caagccgccg atcccgcgcg acgcgatggt gatgttcgcc cagctcccga
      721 agcaccttgc ctggtcgttc atgctcgaca tcgagaaggg cgggatcccg gtcatcccgc
      781 tccggggaac cggccagttc aaccccgacg gcgtgcgcga ctacgacctc ctcgtccccg
      841 catcggccga ggctctgacg atcgagcgcc acacggccga tcgcgagcgc tacctcgccg
      901 cagccgcgcg ctggcgcgag gagtacatcg ccgagttcgg gatcaagccg ccggagaccc
      961 ttcgcccgat gtccgactgg tcgcgggtgc ggccgccgca gcagtgaggg cgctcctcca
     1021 gcggaccacc agcgcccgcg tccgggtggc aacagaggtc gtcggcgaga tctcggccgg
     1081 cctcgtcgtc ctgcttggcg tcgggccgga cgacgacgac gcaacgaccg acgcgctcgc
     1141 ccggcgcgtg accgagttgc ggatcttcga cgacacggcc ggccggacga acctgtcgct
     1201 ggtcgacgtg ggcggcgccg tgctcgtggt gagccagttc accctgtatg cggacacccg
     1261 tcgcggccgc cggccgggct tcaccggcgc cgcggctccg gagctcgccg agcgtctgta
     1321 tctccggttc gcgggcgccc tccgcgacct cggcctgacg gtcgcgaccg gccggttcgg
     1381 cgcggtgatg gccgtcgagc tcgtcaacga cggcccgttc acgatctggc tcgacaccgc
     1441 ggaccgctga tgggcctgct cctgttcgcc ggcctggcga cgttcggcgc aaccgtcgcg
     1501 atcgggatca tcgccgccgg gacgccacgc ccgagcggtg ctgcggtccc gatgccgccg
     1561 tcgcgacgga tggccgtcat tctgttcgcc ctcgcgagcg gcggcggcct ctggggggtc
     1621 ttcgtcgcgc tcctggctgc cgcgtccggc tcgaagggcg gcggcgcctc ggcccgcgac
     1681 gtcgccgggg ccccgctcgt cgccgtgctc ccgatcgtcg gcgccgtcgt ggcgctcgtc
     1741 ctgatgatgc gggcattcag tcgggtcgat ccgtggatcc tcggtcgctc gatccggttc
     1801 atcgtgccgg aggtcgtgct ggcgatcgtc gcggcgatcc tcacggtggt cctggacggg
     1861 ccggccgccg agatggaggc cgggccgttc ctcctgcttg gcttcgtcag cacgggcgcg
     1921 gccatctggc ttggcgtcct aggcgtcggc tccgtccagg cgctcgcagt gctcgatggg
     1981 tccgcgccgg atgactcggt ggtccgggcc gagatcgacc gagtcttcaa gcgatgcgtc
     2041 ccgttcgtga tcgcgtcgat cgcatcagcg gcgctcgcga tcctgctcgt gtcgtcccga
     2101 ctctgaccgg aggatcgcgg tcgggtccgg gcagtccggc cacgctcgac gggtcgacgc
     2161 tcaggacagt cgccatctgg ccagatatgc gccacaggac ccttcggtcg cactcggacc
     2221 agcatcaaag ccaacgactg gcccgagtta gtcgtcggca gctaacaggc gccaagatcg
     2281 gaccgacatc gggctcgaat cgggcatcga tcgagcccag tagtctgtcg acgaatgtct
     2341 ggccaggtgc acctgtccgc ggccgtgcgg gcaagcagcg gacacacgaa cgcccggcac
     2401 ggagccgggc atcaggtcga gcggcgggcg ccggatcggc gccgagcggc tacttcgcag
     2461 ccttcagcga ggtccgccgg cagcgggtgc agacgaggga cttggtctcg tggccgttgc
     2521 tgtcgcggac ggtgaacggc tggaggttcg gctggtagcg acggtgggct cggacgcggt
     2581 tcatccccga cgactgcggg ttgaagccgc ccatcgagac cttgccgcag atcgcacagg
     2641 agcgggccat gttgtcctcg agatcggtcg ggtggtgaga tcgcattcgg gcccgagcgc
     2701 ggacgcacgg gcgtgcccgg ctggaaggcc gcggcggggc ggtatgatag cactcgcgtc
     2761 gcgcgccttg ccacaggccc ggcggatcca gccttgaggg aggatgcccg cggcgtgccc
     2821 accgatccgt cgactccgac ccccggtcgt tcgctcgtca gccgtcgagc gatcgtcgac
     2881 gtggtccgca gcgcgacgct cggatcgtac ggtgtcatcg gctttgccgg cggcggatgg
     2941 ttcggcggta tcgccgagcg gctcggtctg gccccccgcg gcatccaggt ccagctggtg
     3001 ggcggccgcc tcgcgatcga cctcgacctc ctcgtggccc atggtctgcc gatcgcggag
     3061 gtcgctcgcc aggtcgattc tgccgtccgc tacgggatcc gtcgcgcgct cgaccgagaa
     3121 ctcgatcggc tgacgatcca tgtcgacggg ctccggatcg cgccgtacga cgtgcggcgg
     3181 cccggggccg ggcggcagcg ggatgcgctc gaggtcgcgc cagacgatct cgccgcgagc
     3241 ggaaccgacg tcgcctgatg gtccgccagc actgcaccgg agccggcttc ctcgacgcca
     3301 tccggagcgc ggtcgccaac atcgagaagc acgtcgacga gatcaacgcc ctcaacgtct
     3361 tcccggtgcc cgacggagac acgggcacga acatgctggc gacggtccgg gcagccctcg
     3421 acgcggcgga ggccagcggc cgggacgcca cagcggagcg cgtcgccgcg gccgtcagcc
     3481 acggggcgct gatgggcgcc cgcggcaact ccggggtgat caccagccag atcctgcgcg
     3541 gcatcgccga gggtctcgcc ggtcggcggc gcttcaacgg cctcgatctc gccaacgccc
     3601 tcgccctcgg ctcgaagacg gcgtacggcg cggtcggcaa gcccgtcgag ggcacgatcc
     3661 tgacggtcat ccgcgagtcg tcggccgccg cggtcgaggc tgccgagcag gatgacgcga
     3721 tggaatcggt cctggccgcg accgtcgcgg cggccgagaa gtcggtcgcg cgaacgccat
     3781 cgctcctccc gatcctgcgc gaggcgggtg tcgtcgatgc gggcggcgag ggcctcttcc
     3841 gcctgttcga gggggcactg ctgagcgttc ggggccggcc cgtcaacgcc gagtccagcg
     3901 ccgggtcggg acgtctcgag ggcgaggcgc ggacggcggt cgcgccggcg ttccgctcgg
     3961 gcgtggcggc gctcgaggaa gacgccttcg gctacgagac ggtcttcgtc ctcacggtgg
     4021 ccgatggcaa gaccctcgac gtccgggcga tccaatcacg gctggagggt ctcggcaact
     4081 cggtcctcgt cggcggcgat gggcggctgg tcaaggtcca cgtccacaac gagcgacccg
     4141 acaagatcat cgcctatggt ctgtcgctgg ggaccctgac ccggatcacg gtcgagaacc
     4201 tcgacacgat ggccgacgac gttcgcgagg cgcgcgcgtc ggagttcgtc ggcgatgcct
     4261 ccgcggcgga gggttccggc catcggtcac gtggcgccga cagttcaagc gaagcggcgc
     4321 gcggcgcggc cggcgcggtc gcggtcgaag cgcggcccac ggtcgtaccc gggacggcgc
     4381 cggtcaacat ggacgatctg ccgaagccgg gtcggaacgg ttcggacccg tcgtcggacg
     4441 gtcgacccgc ggaaggtccg agcgaggaca cggtctcggt cgacccggcg ctcgggccgg
     4501 cgatcgttgc tgtcgttgcg ggtgacggcc tggagaagct gtttcgcgat ttcggcgtgg
     4561 ctcacatcct gcgtggcggc cagaccgcca atccgagcac gggcgagctg ctgcggatcg
     4621 cccgcctcgc gcgttcccgc gaggtcatcc tgctgcccaa caatccgaat gtccgcctgg
     4681 ccgccgagca ggccgcccgg atctgcgacg atcgtcgtct cgtcgtcgtc ccgacccgca
     4741 acgccgcgga gggcttcgcc gccctgctcg cgtatgaccc cgacgccgac gccgccacga
     4801 acgtcgagcc gatgacgaag gccggccgcg gtctcgcctc gatccaggtc accgaggccg
     4861 tgcgggacgc caggatcggc ggcaagcggg tcaagaaagg ccagacgatc gcgctcgatc
     4921 cggacgacgg tctcgtggcg gtcgacagtg atcgcgacag ggccgtcctc aaggcggtcg
     4981 ggacgttttc cgcgggcgtc gagctcgtga ccgtctacta cggggaggac gccagtctcg
     5041 cggaaaccga ggcgatctcg aagcggatca gcgcccagat cggcgccgag gtcgaggtcg
     5101 tccacggcgg ccagccgcac tatcgctacc tgatctcggc cgagtagggg agtgacccag
     5161 acccgggacg gcttccgccc gccaccgagc ggcccgcgga aagcgaaagc accgtcgccg
     5221 ccaccgccgc cgaccgaccc cgccgagctc ctcgagacga tgctcaatcg ctcggggctg
     5281 ggtgccgcca acgtcctcca gcgggccggc cgccgtctcc gcttcctgac ggtccgcgag
     5341 ctcctcttcc acctgccgcg gcgctacgac gacctgcgcg agatgcgcaa gctcggtgag
     5401 ctccgcttca tcgaggaggg aacggtcgtc agcgcccggg tccgcgtcgt cgatgtccgc
     5461 gtcgagccgt cgttccggcg ccggatccag cggaccatcg ccgagttcga ggacgagacc
     5521 ggctcgatcg aggcgacatg gttcgggcgg cgctacatcg agcgtcgcct gttccccggc
     5581 gcggacatca tcgtgtcggg caagctcaag catttcggtc ggaagctgac cctcgacaac
     5641 ccggacttcc agccgatcgg ggcggagggc gagatgctcc acgtcgggcg gatcgtgccg
     5701 atctaccggt tgactgccgg cctgacggcc gcgcgtctcc ggatcgcgat gcgcgaggcg
     5761 ctcgatcggg ccggcgtcca ctacgaggag tacctgcccg cgtcgatcgt ggccgaggaa
     5821 ggcctcgagg gcatcgcccg cgccctcgag gatgcccact acccctcgac attcgagggc
     5881 cgcgacgcgg cgctgcttcg gctcgccttc gatgagctcc tcgccctcca gctcgggatg
     5941 gtcgggcggc gacggcaacg cggtcgggac gcggcccgcc cgatcgcgat cgacgacgcc
     6001 gcggacgcgg cgctccgtgg ctccctgacc gcggcgctcg gccggaagct gacccgcgcc
     6061 gtggcgctga ccgtcgacca ggacacggcg atcggcgcga ttcgcgacga tctcgcccgt
     6121 gagacgccga tgctccgcct gctccagggc gacgtcggca gcggcaagac cgcggtcgcg
     6181 gcgtgggcgc tggcggcggc cgctcgcaac ggcctccagg gtgcgctcct ggccccgacg
     6241 gaccttctcg cccgccagca tcacgcgacg ctcgcggcgt tgctcgagga cgccgcggtg
     6301 ccggtcgagc tgttgaccgg atcgcagagc accgcccagt cgaagcgcac gctcgagctc
     6361 gtgaagtccg gccaggcgcc ggtcgtcgtc gggacccacg ccctcatcca ggatcgggtc
     6421 gagttcgccg ccctcggtgt cgtcgtcatc gacgagcagc accgcttcgg cgtcgagcag
     6481 cgcggccagc tcgaggcgaa ggccggcggc cacgccccgc acgtcctgct gatgacggcg
     6541 actcccatcc cgcgaacgct cggccaggtc ctgtacgcgg atctcgacgt gtccgacctc
     6601 cgcacccctc cggagggtcg catcgcgatc cggacaggga tccgccaccc cgacaacctc
     6661 ggctcgacct gggagcgtgt ccgggccgag gcggcggccg ggagccgcac gttcgtcgtc
     6721 gttcccctga tcgacgaggc ggagccggcc gacgacgacg gcgcggccaa cccgacgatc
     6781 ttcgactcgt ccgcgccggc tgcggaagcc gaatcggtcc gcctccgtca tctcctcgcg
     6841 ccgctccggg tcgggatggt ccacggccgg atgaagcccg tcgaccgcga caccgagatg
     6901 tcacgcttcc gcgacggcga gctcgacgtg ctcgtcggga cgacggtcat cgaggtcggc
     6961 gtcgacgtcg cggaggcgac gatgatggtc atcgagggcg ccgaccggtt cgggctcgcc
     7021 cagctccacc agctccgcgg ccgggtcggt cgcggcacca cggagagctt ctgcgtcctc
     7081 gtgtccgaca cgatcgacga gaccgcccag gcccgtctca aggccgtcgc cgagatccgc
     7141 gacggcttcg agctcgccga gaaggacttc gagctccgcc gcgagggcga tgtcctcggc
     7201 ctcgtccagt cgggcctgcc gcgtctccgg gtcgcgtcgc tccagaaccg cgagcacgtc
     7261 gccctggcca ggcacgcccg cgaccacgcc gaggcgctcc tcgatgacgc gggccggctc
     7321 ggacccgacg cggcgccgct cctgcgcgag ctcgagcgtg gctggctggc ccgggtctgg
     7381 gccggcgaac ctgcgagcgg agcgtgatcg atggctgacg ccggacgggt catcgcggga
     7441 tcggcgaagg ggatccggct gcgggcaccc ggcgccggga cgaggccgct ggcagaccgc
     7501 gtgaagcaga ccctgttcgc gatcctcgag ccggagctcg acggcgcccg ggtcctcgac
     7561 ctgttcgccg gcagcggtgc cggcgggatc gaggcgctgt cgcgtggtgc ggcggccgcg
     7621 atcttcgtcg agaaggacca gggtgcggcc gcggtcatcg acgccaacct gcgggccacc
     7681 gggctcgcgg gcccgacggt cgccgtcatc cgctgggacg tggtccgctg gctggccgag
     7741 ccgaacgtcg aagccgcgtt cgacctcgtc ctcgtcgatc cgccgtacgc cgagacggag
     7801 ctgctcacgc gcgtcctcga gatcctcggc gcggaccatg ccccgctcgc gcccgatgcc
     7861 cgggtcgtcg ccaagcattt ctggcgcgat cggccgcccg agcggatcgg tagactggcg
     7921 cacgagcggg atcggcgctt cggcgagacc gcgttgacgt tctaccgccg gacgggcagc
     7981 gagccgccgg ccagcgagga gagcggatga cggttgcggt ctaccccggc tcgttcgacc
     8041 cgatcacgaa cggccatctc gacgtcatcg cccgggcgac ggccgtcttc gatcgggtcg
     8101 tcgtcgccgt cctggcgaac ccgcggaagg agccactcct ggcggccgac gagcgcgtcg
     8161 ccatcatccg cgaggcgctc gccgccggcg ccccgggcgt cgccccggag aaggtcggcg
     8221 tcgaagcctt tgatgggctg accgtcgatc tctgccatcg gatcggggcc gggttcatcg
     8281 tccggggcct ccgtgcgatc agcgatttcg agaccgagat gctccttgcc cacaacaatc
     8341 ggcggctcgc ccccgacgtc gacaccgtgt tcttcatgac ctcgatcgag cacagctacg
     8401 tcgcgtcgtc gctggtgaag gagatctcga cgttcggcgg cgacgtatct gcgatgatcc
     8461 cgccggccgc actcgcggcc ctgcggaggg caatcggccc aggctgatcc ggtgccgcga
     8521 acgaggggtg gggatggacg gaagcggagc ggtcatcgag acccgcggcc tggtgaaacg
     8581 gttcggggac gtgaacgcgg tcgacggcat cgacctcgac gtccgggccg gcggcgtcta
     8641 cggcttcctc gggccgaacg gtgccgggaa gacgacgacg atccggatct tcgccgggct
     8701 gctctggccg acgcgcggat cgacgcggat tttcggtgag ccggttgccc ccgaggcggc
     8761 ggtcctcgcc cgggtcggct cgctgatcga gcgaccggcg ctctacccct acctgtcggc
     8821 gttcgagaac gtccgcgtgt tcgggacggc ccatggcctc gggcccgacg tgctggtcca
     8881 gcgggcggcc gaatcgctcg atcgggtcgg gctggcggcg gtcgcgcgac ggcacgccgg
     8941 cgggttctcg accgggatgc gccagcggtt ggccctcgcg atcgcgctga tcggccggcc
     9001 cgacctcgtg atcctcgacg agccgacgaa cgggctcgat ccgaacggcg tcgtcgacgt
     9061 ccgcgagctg atcgctcgac tcgccgccga cgggaccacg gtcttcctct cgagccacgt
     9121 cctgcccgag gtcgagcagc tctgcgaccg ggtcgcgatc ctgcgcgagg gccggatcgt
     9181 cgccgagggc ccgaccggcg acctcctcgg gaccggcgag cggttgtacg tccgctttga
     9241 cactccggcg gaggcggccg cggccctggc gatcctcggc gcgaacgcga cgccggcgga
     9301 cgccggaccg acccatccgg gatcggccct gatcgtcgac gcgccgatcg cggaggcctc
     9361 gcggatcaac cggacgctgg gtgcggcggg cctctacccg gccgagctcg cgccccgccg
     9421 cgaatccctc gaggcggtct ttcgcgagct cacctcggac cacccggacg ggccgccgcc
     9481 gacgaccccg gcgccgccac cggtcgccgc cgcgccgacc gccggcccgg cccgatgacg
     9541 gcgctgctcc gcgccgactg gctgcgcttc cggcgccggc gcgacttctg gatcatcgcg
     9601 atcgccgtgg cgctgatcgg cgggatcggc ttcctggcga gctaccggag cgaggtccag
     9661 gatccggcgc cgttcaacga ggccgagttc cgccagcagg ccatcgacgg cggcttcttc
     9721 gagggcgtcc ccccggagga gctccaggcg cagatcgatc agttcgccgc cgatatgcgc
     9781 gcctccgacg agcagaaccg gatcgagtgg gaccgggaac aggcgatcaa cctccagaag
     9841 tacgacatcg cgcagagccc gttcacgatc atcggggtcg ggatcgcccc gctgatcgcg
     9901 ctgatcctcg ccgcgagcct cgcggtcggc gacgagttcc ggtt
//
LOCUS       DATAIW010000407         3966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_250288, whole genome shotgun sequence.
ACCESSION   DATAIW010000407 DATAIW010000000
VERSION     DATAIW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250288"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..>3966
                     /locus_tag="VHM48_12095"
     CDS             279..>3966
                     /locus_tag="VHM48_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2756202.1"
                     /translation="MATVSIPRREPTAAIPGGDPLAPFSPAVRAWFESSFAAPTQAQT
                     EGWAAIANGHHTLIHAPTGSGKTLAAFLWTLDRLAASPPRPAPTKADPGSIRVLYVSP
                     LKALTYDVERNLRAPLAGIALAAARLGEPAPDITVASRTGDTPAEDRRNIARRPPDIL
                     ITTPESLYLMLTSQAREVLRGVEHVIVDEVHAIAGSKRGAHLALSLERLDHLVAERQG
                     STPPQRIGLSATQRPLELIARFLGGVGPDRAVTIVDAGSRKALDLQVIVPIEDMTKPG
                     ELLPLDQQPGGPATAPEARVSIWPAIHPRILELIREHRSTIVFTNSRRLSERLAQRLN
                     ELAGEELVRAHHGSVAREQRVQIEEDLKAGRSPAIVATSSLELGIDMGAVDLVIQVES
                     PTSVARGLQRVGRAGHQVGAPSKGVIFPKYRGDLLEAAVVTRRMHEGAIEATAIPRNP
                     LDVLAQQVVAMTVMDSWTVDDLLATMTRAMPYETLSREVLEGVLGMLSGAYPSDEFAE
                     LKPRVTWDRATDVIAGRRDARVVAVTSGGTIPDRGLFGVFMVGEAGTPGRRVGELDEE
                     MVYESRTGEVITLGASSWRIEEISHDRVTVSPAPGVPGKLPFWHGDAVGRPIELGRAL
                     GAFIGEAEADLGRGPRGRTAVIKRLRDHHHLDAWAAENLVAYLEDEREAAGALPTDHR
                     IVVQRFRDELGDWRLVLLTPFGGRVHAPWSLALEARLGERLSAEIRTIWSDDGIAIRL
                     PDGDDVVGIAEELLFPDPEEVEDIVVGQVASSALFASRFRENAARALLLPRRRPGTRT
                     PLWQQRQRAADLLAVASRYGSFPILVETYREVLSDVFDLPALREVLGGIARREITIHS
                     VETPKASPFASSLLFDYVAAYMYDGDAPLAERRAGALTLDRDLLRELLGQEELRELLD
                     PEALADLELNLQSLAEDRQAATADQLHDLLRRLGDLSAGEIAARCEGGAAAGDAWLTE
                     LIGSRRAVAARIAGDDRWIAIEDVARYRDGVGVSPPTGVPAAFLGPAVGALDGLLARF
                     ARTHGPFLTPEPARRWGLPFGVVGDALERLLDAGTILRGEFRPGGAERDWIDPDVLRL
                     LRRRSLARLRKEVEPVDPAAFARFLPAWQGVAAIGEAPVPLRQNAALERLAEVIDQLA
                     GVALPASVLERDILPARVPGYQPRLLDELGALGEVTWVGRGSLGRDDGRIVLVRPGRE
                     LLRPSGPVDADARPADLRHEKIREH"
BASE COUNT          531 a         1492 c         1391 g          552 t
ORIGIN      
        1 gatcctgccg tctctgccag ccacatggcc gggatgcgtg aacggcgttg atctctggtc
       61 ctactgtcct ttcccgaccg cgtgccgctg acgggtcgat cggcctgtcc tcagggggcc
      121 gatgggcacg acatcctgtg acgggacgat cggaactggg accacatcgc gcagcgtagt
      181 ccgtcgcgca agtggccaga tgacgacccg tcatctcatg ggtccatatt tcgaacgggt
      241 ggtcgatttg cacgcggccg cctatactgc cgagtcccgt ggccaccgtc tcgattccgc
      301 gtcgcgaacc gaccgccgcc attcccggcg gtgacccgct ggcgcccttc tcgccggccg
      361 tccgggcctg gttcgagagt tcgttcgccg cgccgaccca ggcccagacc gagggttggg
      421 ccgcgatcgc caacggccac cacaccctga tccacgcgcc gaccgggagc ggcaagaccc
      481 tggccgcgtt tctctggacc ctcgaccgcc tcgcggcatc gccaccccgc ccggcgccca
      541 cgaaggccga tccgggctcg atccgcgtcc tctacgtcag cccgctgaag gccctcacgt
      601 acgacgtcga gcggaatctc cgggcgcccc tcgccgggat cgcgctcgcg gcggcgcggc
      661 tcggtgagcc ggcaccggac atcacggtcg ccagccggac cggcgacacc ccggccgagg
      721 accgccgcaa catcgcccgc cggccgccgg acatcctcat cacgacgccc gaatcgctgt
      781 atctcatgct cacaagccag gcccgcgagg tcctccgcgg ggtcgagcac gtgatcgtcg
      841 atgaggtcca cgcgatcgcc ggatccaagc gcggcgccca tcttgccctc agcctcgagc
      901 gcctggacca cctcgtcgcc gagcgtcagg gctcgacacc gccgcagcgg atcggcctca
      961 gcgccacgca gcggccgctg gagctcatcg cccggtttct cggcggcgtc gggccggacc
     1021 gcgcggtcac gatcgtcgac gccggcagcc gcaaggcgct cgacctccag gtgatcgtcc
     1081 cgatcgagga catgacgaag cccggcgagc tgctcccgct cgatcagcag ccgggcgggc
     1141 cggcgaccgc gcccgaggcg cgcgtcagca tctggccggc gatccacccc cggatcctcg
     1201 agctcatccg ggagcaccgc agcacgatcg tcttcacgaa cagccggcgc ctgtcggagc
     1261 gactcgccca gcgcctcaac gagctggccg gcgaggagct ggtccgggcc caccacggat
     1321 cggttgcgcg cgagcagcgc gtccagatcg aggaggacct gaaagccggt cgctcaccgg
     1381 cgatcgtcgc gacgagcagc ctcgagctcg ggatcgacat gggggcggtc gatctcgtca
     1441 tccaggtcga gagcccgaca tcggtcgccc gtggcctgca gcgggtcggg cgggccggcc
     1501 accaggtcgg cgccccgagc aagggcgtga tcttcccgaa gtaccgtggc gacttgctgg
     1561 aggccgcggt cgtcacccgc cggatgcatg agggcgcgat cgaagcgacc gccatccccc
     1621 ggaacccact cgacgtgctt gcccagcagg tcgtggcgat gacggtcatg gacagctgga
     1681 ccgtcgacga cctgctcgcg acgatgaccc gggcgatgcc ctacgaaacg ctgagccggg
     1741 aggtcctcga gggggtgctc ggcatgctct cgggcgccta cccgagcgac gagttcgccg
     1801 agctcaagcc tcgggtgaca tgggaccggg ccaccgacgt gatcgccggc cgtcgggacg
     1861 cccgggtcgt ggccgtgacg agcggcggga cgatcccgga ccgcgggttg ttcggggtgt
     1921 tcatggtcgg cgaggccggg acgcccggcc ggcgggtcgg cgagctcgac gaggagatgg
     1981 tctacgaaag ccggacgggc gaggtgatca cgctcggcgc cagcagctgg cggatcgagg
     2041 agatctcgca cgaccgggtc acggtctcgc cggcgcccgg cgtgcccggc aagctgccgt
     2101 tctggcacgg cgatgcggtc gggcggccga tcgagctcgg gcgagcgctg ggcgcgttca
     2161 tcggcgaggc ggaggccgac ctcggccgcg gtccgcgcgg gcgaacggcc gtgatcaagc
     2221 gactccgcga ccaccaccac ctcgacgcct gggctgccga aaacctggtc gcgtacctcg
     2281 aggacgaacg cgaggccgcc ggcgcgcttc cgaccgacca ccggatcgtc gtccagcgat
     2341 tccgcgacga gctcggcgac tggcgcctcg tgcttctgac accgttcggc ggccgggtgc
     2401 acgcgccgtg gtcgctcgcc ctggaggcgc gcctcggcga gcggctcagc gccgagatcc
     2461 ggacgatctg gtccgacgac gggatcgcga tccgcctgcc ggacggcgac gacgtcgtcg
     2521 ggatcgccga agagctgctc ttccccgatc ccgaggaggt cgaggacatc gtcgtcggcc
     2581 aggtcgcgtc atccgcgctg ttcgcctcgc gcttccgcga gaacgcggcc cgggctctcc
     2641 tccttccccg gcgccggccc gggacgcgga ccccactctg gcagcagcgc cagcgagccg
     2701 cggacctgct ggcggtggca tcccgatacg ggagctttcc gatcctcgtc gagacctatc
     2761 gcgaggtcct gtccgacgtc ttcgacctgc cggccctgcg cgaggtcctc ggcgggatcg
     2821 cccgccgcga gatcacgatc cacagcgtcg agaccccgaa ggcgagcccg ttcgcgagct
     2881 cgctcctgtt cgactatgtc gcggcctaca tgtacgacgg cgatgctccg ctcgccgaac
     2941 gtcgcgccgg cgccctgacc ctcgaccgcg atctcctccg cgaactgctc ggccaggagg
     3001 agctccgcga gctgctcgac cccgaggccc tggccgatct cgagctgaat ctccagtcgc
     3061 tcgccgagga ccgccaggcc gcgaccgcgg accagctcca cgacctgctc cgccggctcg
     3121 gcgacctctc ggccggcgag atcgccgccc gctgcgaagg cggtgccgct gccggcgacg
     3181 cgtggttgac cgagctgatc gggtcgcgcc gagccgtcgc ggcgcggatc gccggcgacg
     3241 accgctggat cgcgatcgag gacgtcgctc gctaccgcga cggggtgggg gtgtcgccgc
     3301 cgaccggcgt cccggccgcg ttcctcggcc cggccgtcgg cgcgctcgat gggctgctgg
     3361 cgcgcttcgc ccggacccat ggcccgttcc tcacgccgga gcccgcccgc cgctggggac
     3421 tgccgttcgg cgtggtcggc gacgcgctcg agcgcctgct cgatgccggg acgatcctgc
     3481 gcggcgagtt ccggcccggc ggcgcggagc gtgactggat cgaccccgat gtcctccggc
     3541 tgctgcgccg ccgctccctg gcccgcctcc gcaaggaggt cgagccggtc gaccccgccg
     3601 ccttcgcccg gttcctgccg gcctggcagg gggtcgcggc gatcggtgag gcgccggtgc
     3661 ccctccgcca gaacgcggcc ctggagcgcc tcgccgaggt gatcgaccag ctcgccggcg
     3721 tggcgctgcc cgcatcggtc ctcgaacgcg acatcctgcc ggcccgcgtc ccgggctacc
     3781 agccgcgcct cctcgacgag ctgggtgccc taggcgaggt gacctgggtg gggcggggga
     3841 gcctcggtcg cgacgacggc cggatcgtcc tcgtccggcc gggtcgtgag ctgctccgcc
     3901 cgtccgggcc cgtggacgcg gatgcccggc cggccgatct gcggcacgag aagatccgcg
     3961 agcacc
//
LOCUS       DATAIW010000408         5182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_220733, whole genome shotgun sequence.
ACCESSION   DATAIW010000408 DATAIW010000000
VERSION     DATAIW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220733"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VHM48_12100"
     CDS             <1..403
                     /locus_tag="VHM48_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756203.1"
                     /translation="DVVQDAFLGVWRNAGRYVEGRGSVKTWVLSIVHHRAVDAVRRRR
                     PTTELPEREDVPPPALQSPDIWHDVAAGLDREEIAAAMRTLSDVQREAIELAYWGGLT
                     QQEISERTGTPLGTVKSRVRLGLLSLDDARA"
     gene            complement(728..1423)
                     /locus_tag="VHM48_12105"
     CDS             complement(728..1423)
                     /locus_tag="VHM48_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756204.1"
                     /translation="MSRAAASIVALGLVVAACGSMVVGPTADPPLVKAPYTLAPPTPT
                     LRVLATVGPGGPGKPYTADDILAILRAAPAGDPAEMRTPQIAAAIADRVWTFDGRPYQ
                     RLLVGGRCDGGMCEISAEGMPAFGATDDLADDWFWRMDLRTGLVSDNGEPRLRGFPPE
                     LVPELDALARSLDTDGTFSALPLIRVEWTVPPPVGAFVLRYGTGTQEMDVNWFVKIST
                     VDRAILSIRKQVM"
     gene            complement(1429..2412)
                     /locus_tag="VHM48_12110"
     CDS             complement(1429..2412)
                     /locus_tag="VHM48_12110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2756205.1"
                     /translation="MPDQPDRPAPLDALAAGVAAAVRGADLDDGIGTLVGAAIAALGA
                     TSAMVSLQDPDRPDPELTLTIGLDEAAQQAAVAGVADPAHPLTIAGRDRTEARSGPTI
                     ARPLIVTRAGVEEALGALAVTFADDASADAADPTYLGLVADLAALAVDRARLASTVAE
                     RSEWFERLAHTDPLTGLANLRTVSRVLELELARAGRQGGEVSVAIFDVDDFTAVNAAA
                     GRDAGDDILRSVAAVLSGSVRLVDTVARYGADEFLLVAPGSAGAMVAQRVLDGVAELP
                     ATDGRTISVTAGVARFPTDGSDAEGVMAAALGALDRARADRRGTVETSEAG"
     gene            2578..2907
                     /locus_tag="VHM48_12115"
     CDS             2578..2907
                     /locus_tag="VHM48_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX2756206.1"
                     /translation="MKLVVAIVHHEDAGALVDALLDREFRATRLSSSGGFLKQSNTTV
                     ILGVEDDAIDEVLGIVRETCHSRTQVVNPMPPIMEPGEFFMPYPLEVEVGGATVFVLP
                     VERFERI"
     gene            2940..3557
                     /locus_tag="VHM48_12120"
     CDS             2940..3557
                     /locus_tag="VHM48_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756207.1"
                     /translation="MTDDWVAALERDAPDLVAAWRGIEAGRFVTVRREVRRRAGPGSA
                     DPILAQLEVTATALTAIIATLPDDAFAMPGGEADWTVAEAIGHDAAARAGLVMAGALA
                     ASGRWPADVPTVVPSVPGPAESGRDELVRKVLQSKRIIARSAGTIVGHEADPCPLDHP
                     LVGRLRCGEWLLFAGVHDVMHLEQLHRIAATLEPAPQPSPGQPVG"
     gene            3589..4764
                     /locus_tag="VHM48_12125"
     CDS             3589..4764
                     /locus_tag="VHM48_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2756208.1"
                     /translation="MLDQTASASANRRVFRSKVDGLRPTYGFEDVSLAPGTDTIEPAD
                     VELGQEFCGITLGIPIIASAMDAVVDPRFAGALAALGGLAILNLEGVQTRYEDPDEIL
                     GRIAAAADGDVQALLADVYASPIREELIARRLDEIHAAGSKAAVAATPGAARRFGPFC
                     AEHGADLFLVQSQVSSARHLASGYDPLSLEEFTRFMPIPVAVGNTTNSEAAFALMEQG
                     AAAVFVGVGPGAACTTREVLGIGVPQVTAISDVAAARDAFHAETGRYVPVVADGGMRR
                     GGELAKAIAAGADVLMIGSPLARADEAPGRGMNWGMAAPSPTLPRGIRIKVGTVGSLE
                     QILLGPAHVTDGSENLIGALRQSMAALGAATIRDMQRVEMVYAPSVATEGKSWQRSR"
BASE COUNT          704 a         1904 c         1871 g          703 t
ORIGIN      
        1 ggacgtcgtg caggacgctt ttctcggcgt ctggcgcaac gcgggtcgct atgtcgaggg
       61 gcgcgggagt gtcaagacct gggtcctctc gatcgtccac caccgggcgg tcgacgccgt
      121 ccgacggcgc cgcccgacga ccgagctgcc ggagcgcgag gacgtcccac cgcccgctct
      181 ccagtcgccc gacatctggc acgacgtcgc ggccggcctc gaccgcgagg agatcgccgc
      241 cgcgatgagg accctgtcgg acgtccagcg cgaggcgatc gagctcgcgt actggggcgg
      301 cctcacccag caggagatct ccgagcggac cggaacgccg ctcggcacgg tgaagagccg
      361 cgtccggctc gggctgctgt ccctggacga cgctcgggcg tgaacctcgc gaggctcgtt
      421 ggcgactcgc ctgtcagccc tccacgacag cacgcagccg cgcggagatg tgccgcgagg
      481 cccgaacgga tggggtttcc tgccatccgt ccagggtggc gaccttcggc gcgcttcccg
      541 cgatgatcgg ccggagccgt cgccgctgct gagcgtggat cgggccgtgg cgtgctcatt
      601 cggagccgtt cgacccgcct gcaacacccg acatcgcgcc caaggtcttc tcacgcgact
      661 tctggcagga aaatcgtcgc atccgatccg atcctccggc ggcgcgacca tcgcgccggg
      721 caggtcgcta catcacctgc ttccggatcg acaggatcgc ccggtcgacg gtcgagatct
      781 tgacgaacca gttgacatcc atctcctgag tgccggtgcc gtagcgcagg acgaacgcgc
      841 cgaccggtgg cgggaccgtc cactcgacgc ggatcagcgg cagagcgctg aacgtgccat
      901 cggtgtcgag cgatcgggcg agcgcatcca gctccgggac cagctcgggt gggaagccgc
      961 gcaggcgggg ctcgccgttg tccgagacca ggcccgtccg caggtccatc cgccagaacc
     1021 agtcgtcggc gaggtcatcg gtcgcgccga acgcgggcat gccttcggcg ctgatctcgc
     1081 acatcccgcc gtcgcacctg ccaccgacga ggagccgttg atacggtcgg ccgtcgaacg
     1141 tccagacgcg gtccgcgatc gccgccgcga tctgtggcgt gcgcatctcg gccggatccc
     1201 cggccggcgc ggcgcggagg atcgccagga tgtcgtcggc cgtgtagggc ttgccgggtc
     1261 cgcccggccc cacggttgcg agcactcgga gggtcggcgt cggcggggca agggtgtacg
     1321 gcgccttgac gaggggtggg tccgcggtcg gaccgacgac catcgacccg caggcagcca
     1381 cgaccagccc gagcgccacg atgctggccg cggcgcggct catccggatc agccggcctc
     1441 gctggtctcc acggtgccgc gacgatcggc gcgggcccgg tcgagggcgc cgagggcggc
     1501 ggccatgaca ccttcggcat cgctgccgtc ggtcgggaag cgggcgacac cggccgtcac
     1561 cgagatcgtc cgaccgtcgg tcgcgggcaa ctcggcgacg ccgtcgagga cgcgctgggc
     1621 gaccatcgcc ccggccgacc cgggggcaac gagcaggaac tcgtcggcgc cgtatcgggc
     1681 gaccgtgtcc accaggcgaa cggatccgga cagcaccgcg gccacggagc ggaggatgtc
     1741 gtcgccggca tcgcgacctg cggccgcgtt caccgcggtg aagtcgtcga cgtcgaagat
     1801 cgcgaccgac acctcgccgc cctggcgacc tgcccgcgcc agctcgagct cgaggacgcg
     1861 cgagaccgtc cggaggttgg cgagcccggt caggggatcg gtgtgggcga ggcgctcgaa
     1921 ccattccgag cgctccgcga cggtcgaggc aagacgcgcc cgatccaccg cgagggccgc
     1981 caggtcggcc accaggccca ggtacgtcgg gtcggcggca tcggcggacg catcgtccgc
     2041 gaacgtcacg gccagcgcgc cgagcgcctc ctcgaccccg gcccgggtca cgatcagggg
     2101 ccgggcgatc gtcgggccgg atcgggcttc ggtccggtcg cggccggcga tcgtcagcgg
     2161 gtgggccggg tcggcgaccc cggcgaccgc cgcctgctgg gccgcctcgt cgagcccgat
     2221 cgtcagcgtc agctcggggt ccggtcgatc gggatcctgg aggctgacca tcgcgctggt
     2281 cgcgccgagg gccgcgatcg cggcgccgac gagcgtgccg atcccgtcgt cgaggtctgc
     2341 gccgcggacg gcggctgcca caccggcggc gagggcgtcg agcggagcgg gtcggtcggg
     2401 ctggtctggc acggaggagt ctcctggacg cggatcgggc acggaggcga gcggtcgcgc
     2461 ggttcgcgca cacgcggccg ccgcatcgta caccgcaccg cgacgcacca gaccacccct
     2521 cggatgaggg atgcgaacgg cccccgtcgg gctcgtcgcc gtgcctatac tcggcgcgtg
     2581 aagctcgtcg tcgcgatcgt ccaccacgag gacgcgggtg ccctcgtcga cgccctcctg
     2641 gatcgagagt tccgggcgac ccggctctcg agctcgggcg gattcctgaa gcaatcgaac
     2701 acgacggtga tcctcggggt cgaggacgac gcgatcgacg aggtcctcgg catcgtccgc
     2761 gagacgtgcc actcccggac ccaggtcgtg aacccgatgc cgccgatcat ggagccgggc
     2821 gagttcttca tgccgtaccc gctcgaggtc gaggtcggcg gggcgacggt cttcgtgctc
     2881 ccggtcgagc ggttcgaacg aatctgaggc ctgcgggagg gccgcgctac gctgcgacga
     2941 tgacggatga ctgggtcgcg gcgctcgagc gggacgcgcc cgatctcgtc gcggcatggc
     3001 gcgggatcga ggccggtcgg ttcgtcacgg tccgccgcga ggtccggcga cgggccggcc
     3061 ccggatcggc cgatcccatc ctcgcccagc tcgaggtcac ggcaaccgcc ctcacggcga
     3121 tcatcgcgac cttgcccgac gacgcgttcg cgatgcccgg tggcgaggcc gactggacgg
     3181 tggccgaggc gatcggccac gacgccgcgg cgcgcgcggg attggtcatg gccggtgccc
     3241 tcgccgcgtc gggtcgctgg ccggcggacg tgccgacggt cgtcccgagc gttccggggc
     3301 ccgccgagtc cggccgggac gaactcgtcc ggaaggtgct tcagagcaag cgaatcattg
     3361 cccgctccgc cggaacgatc gtcggccacg aggcggatcc ctgtccgctc gaccacccgc
     3421 tcgtcggccg gttgcgctgc ggcgagtggc tcctgttcgc gggcgtccac gacgtgatgc
     3481 acctcgagca gctccaccgg atcgctgcga cgctcgaacc cgcgccgcaa ccgtcacccg
     3541 gccagcccgt cggctaacat cgcagcgacc gccgccagga gaccgcccgt gctcgatcag
     3601 accgccagcg cctccgccaa ccgacgagtc ttccgctcga aggtcgacgg cctccggccg
     3661 acctacggct tcgaggacgt gtcgctggcg ccgggcaccg acacgatcga gccggccgat
     3721 gtcgagctcg gtcaggagtt ctgcgggatc accctcggca tcccgatcat cgcctcggcg
     3781 atggacgcgg tcgtcgaccc gcggttcgcc ggcgcgctcg cggcactcgg tggtctcgcg
     3841 atcctcaacc tcgaaggagt ccagacccgc tacgaggatc ccgacgagat cctcgggcgg
     3901 atcgcggccg cggccgacgg cgacgtccag gccctgctcg cggacgtgta cgccagcccg
     3961 atccgcgagg agctgatcgc ccgccggctc gacgagatcc atgccgccgg ctcgaaggcc
     4021 gccgtcgcgg cgacccccgg tgcggctcgc cgcttcggcc cgttctgcgc cgagcacggt
     4081 gccgacctct tcctcgtcca gagccaggtg tcctccgccc gccacctggc gtccggctac
     4141 gacccgctct cgctcgagga gttcacccgg ttcatgccga tcccggtcgc ggtcggcaac
     4201 acgacgaaca gcgaagccgc cttcgccctc atggagcagg gcgccgccgc tgtcttcgtc
     4261 ggggtcggtc caggtgccgc gtgcacgacc cgcgaggtgc tcgggatcgg cgttccgcag
     4321 gtcaccgcga tcagcgacgt ggccgcggcg cgcgatgcgt tccacgccga aaccggccgc
     4381 tacgtcccgg tcgtcgccga cggcgggatg cgccgcggcg gcgagttggc gaaggccatc
     4441 gccgcgggcg ccgacgtcct gatgatcggc tctccactgg cccgcgccga cgaagcgccc
     4501 ggacgtggca tgaactgggg catggccgcc ccctcgccga ccctgccgcg cgggatccgg
     4561 atcaaggtcg ggacggtcgg gtccctggag cagatcctgc tcgggccggc ccatgtgacc
     4621 gacggctcgg agaacctcat cggcgccctg cgccagtcga tggccgcgct cggggccgcg
     4681 acgatccgcg acatgcagcg ggtcgagatg gtctacgcgc cgtcggtcgc gaccgagggc
     4741 aagtcctggc agcggagccg ctgacggctc gcgacgaccg gcccgacgag ccggcgcgcg
     4801 cagcccgccc ttcttcgggg ccggcgcgcg gcaccgagcg cgtcgccgcc ctcgacgacc
     4861 tcctcgccgg cgacagccga ttcgctcgtc tccggacccg cggccggcgg agcggcgaat
     4921 ggcgtctcgt cacggtcggc ttcgtcaacg agcccgacgg ctcgatcctc gtcgccgcgg
     4981 gcgcgcccga cagccagtgg gcccggaatc tgctggacga tccgaccgtc gaggtcgtgg
     5041 ccggcgggcg gtcctacctc ggcgttgcgg aggccctcga cgatcgcgac ccacgccgcg
     5101 gccgtgccgt gcgcgagctg atcctgcgct acgggacgcc gtccgagggg ctcggcagcg
     5161 gcccgatctt cgtcatccgg cc
//
LOCUS       DATAIW010000409        13922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_280362, whole genome shotgun sequence.
ACCESSION   DATAIW010000409 DATAIW010000000
VERSION     DATAIW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13922
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280362"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..134
                     /locus_tag="VHM48_12130"
     CDS             9..134
                     /locus_tag="VHM48_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2756209.1"
                     /translation="MTPEDVGKTALYLASDLSSAVTGEVIYVDGGFSVMGVPSGD"
     gene            complement(593..1207)
                     /locus_tag="VHM48_12135"
     CDS             complement(593..1207)
                     /locus_tag="VHM48_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756210.1"
                     /translation="MSDRAGMPHAAATMPVDLIAFTADRRIHGAIPLADDRLSDMLNS
                     VARIVIRGAVIEDLIGDAALETADLTLAVGSIVAVLVEGRRGIDSRRRKTEVHRARVG
                     LTRFVVSGSLHVPIGAADRLAAGNPAAVFVGRDILVPLTDATITYDKADVPTTEHFET
                     ILVNRAHVTWVDLDGAAGSDDSDLVEERPKVYHASIVKDFTRAT"
     gene            complement(1243..1977)
                     /locus_tag="VHM48_12140"
     CDS             complement(1243..1977)
                     /locus_tag="VHM48_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2756211.1"
                     /translation="MNLLDVIVTIASVTARSLLGRRRSILMLLLAGAPVLLGLLVRIG
                     GTRSGALPSALEGLVVSAVLPLVALVFGTAALGSELDDGTAVHVLTKPIPRWAIVLPK
                     FVVAGGMTAVLLFPATILAGILVGGLGSRELGLTFAFAIAVLIGSFVYSAIFVALSAA
                     TSRGLVIGLGYSLLWEGLLSGALKGTQLFSVREYIRGMASTLAPRGAIDSLVGANGFL
                     FAAVAIVVITALASWRLARYEIRAAE"
     gene            complement(1974..2921)
                     /locus_tag="VHM48_12145"
     CDS             complement(1974..2921)
                     /locus_tag="VHM48_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756212.1"
                     /translation="MPLASPTLVDPNQPIVLDRVSRWYGNVVAVNDISFSLGSGITGL
                     LGPNGAGKSTLLHMLAGLLRPSSGRVLIGGRPAWHEPSIYRDIGLVPEREAVHSFLTG
                     RQFVDLAARLQRLPNPAAATERAIATVDLASVADRAVGTYSKGMRQRVKIAAALVHEP
                     SILLLDEPFNGMDPRQRLHMMDLLRSMAAAGRTILFSSHILEEVERLADGILVIHAGR
                     LAAAGDYREIRRLMTDRPHTFRVRSSDDRRLASAFLAEPGVFGAELRDDRLAVSVSEF
                     SGFTRIVARVARDAGVRLFEVAPTDDSLESVFEYLVTRR"
     gene            complement(2963..3877)
                     /locus_tag="VHM48_12150"
     CDS             complement(2963..3877)
                     /locus_tag="VHM48_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756213.1"
                     /translation="MSALPPVTRAGNIYDLGYRRYEGQRLGRAHAIRSLVVHSFRTTF
                     GIGRSGRAKAAPFIFGTMAILPAVIIVGGLTIAARFGFDRQLDDTELIGFNNYYSSVT
                     AIVVLFCAAQAPELFGRDQRHGVLALYFARALRRSDYALGRLLGFMLAVLVLLLIPMI
                     ILFLGRILLSTDIAGSLGKNLPSVPPVVAQGAVIAALYGGLAMAISAYTPRRAYATAG
                     IIALFVLPGLIAGIVIQIGSSAVGTGLVLISPNTIIDGTNAFVFGGPLGGEYLFVDLP
                     HWAFLVAAIVEVAVVVALILRRFARITI"
     gene            complement(3874..4836)
                     /locus_tag="VHM48_12155"
     CDS             complement(3874..4836)
                     /locus_tag="VHM48_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756214.1"
                     /translation="MSLIATTALTKRYPGGVTALDGLTVEIEPGVVGLVGSNGAGKST
                     LLKILLGLLEPTSGSATIFDLPVATRGIEIRELIGYMPEHDCLPPDMTATDIVSHLAL
                     ISGLPKAAARERTAEVLRHVGLYEERYRAIGGYSTGMKQRVKLAQALVHDPRLLLLDE
                     PTNGLDPAGRDEMLELIHRTGTEFGIAVIVASHLLGEIERVCDHLVAIEGGKLLRSAP
                     LHSFTQRTGSLAVEVEEGREALVERLTARGLRVVVDGAAILVDLVDETPFDIVRDTVV
                     ELDLALVRMEQRRHRLEDLFRDDPAAAAAAVQPSAETLPPAVGR"
     gene            5055..6389
                     /locus_tag="VHM48_12160"
     CDS             5055..6389
                     /locus_tag="VHM48_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2756215.1"
                     /translation="MSPDVAERQLLGAELWERGDATTSARFLAGWEDGATFCAAVCST
                     DLGLAFCRACPTDVANRALANRRATSGRCPAGVRLLAFPAPAGSVDRVAVLRVGPPTA
                     RQAVAVTDQVRVAPTALRRAAREMEPADGRASLSAARRLRDPAGNRDWQVEQRARGAD
                     RRRAAGQALAQMIATSEELHELYRASQRQRRQLDRNRRLVDGLARATLRAQDEERSRI
                     AHEIHDTAAQSMVSAFRFLDAARAASGANGHEPDPRLVHASERLQSAIREVRAVLARL
                     LPPGLEELGLADALRTRLNTAHADDGSHSTTGAVSGDLPRLKAWVEQALYGMASEAVS
                     NAVRHGQATTIGIDLGVVRGRAVVTVTDDGGGFDPTTVIRTEGSGLGLVGLTRQASWL
                     GGSARIVSRPGGGTTVRISIPLARHLRGSSGDEAGSEADATAVESRRSGEGQ"
     gene            6428..7093
                     /locus_tag="VHM48_12165"
     CDS             6428..7093
                     /locus_tag="VHM48_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756216.1"
                     /translation="MTERRRVVLIDDHEMARRGLESMLSTANWIDVVGDADDCAGGLA
                     AIERFRPDIVLLDIRMPGTDGLACLDRIKARADPVAVVMVTLYDDRRYVLEAIRRGAA
                     GYLLKDASTDDVIATLQRVADGQLAIDPQLLREALTTRDDLPARATPRQRAEAYRVTP
                     REHDVLALVAEGLTNKEIGSRLSISEDTVKKHVQNIIWKLRAADRTQAAITAFRMGLL
                     EDA"
     gene            7177..7608
                     /locus_tag="VHM48_12170"
     CDS             7177..7608
                     /locus_tag="VHM48_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2756217.1"
                     /translation="MSDAEKVVRHRAPIILVVDDDEYVHSTLAAAMRSLRPDILWAAT
                     AAAGLALALDRRPDLAIVDVGLPDLDGYALVRAIRARPELAGLRVCILTGHLPDETLA
                     RDVGANAIIGKPFRLDEFLATIRGQLRIRPGDAAGARPVVG"
     gene            complement(7668..8279)
                     /locus_tag="VHM48_12175"
     CDS             complement(7668..8279)
                     /locus_tag="VHM48_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756218.1"
                     /translation="MMKLFGSSRPRPGAAGAAPGRQATTAVPVREATDPVCLQCGMIA
                     AGTDVSFCRRCGLPIGAAPRAEAELPSCPTCYATVDDDGLIASCRQRTSRIDLVAHMV
                     EHEQYPVGDDDYLESLRSGDLIRIGRWQAPYDLVRRYLVTGAIDGGRRRSYQHSAIVT
                     AMSQLKRWGPEAEIFGDQEEWRAARQAVGDLLERFNIRAASRA"
     gene            complement(8276..8644)
                     /locus_tag="VHM48_12180"
     CDS             complement(8276..8644)
                     /locus_tag="VHM48_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756219.1"
                     /translation="MNTYESDASIESSRIHADDVEQSFLEVHGIEGSILRATRLGLTV
                     YPSRSSAWAADGDRRWSYEQLREVRLDAYGPVGVIRATVRSGGLLPLLLLEPEQIAAA
                     RRTLEIIWNLMASAHHTRRS"
     gene            complement(8782..9156)
                     /locus_tag="VHM48_12185"
     CDS             complement(8782..9156)
                     /locus_tag="VHM48_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756220.1"
                     /translation="MNTPRRLTTVTRRLTTALRVALRRRPRTVFAAGLLIVIVALVGS
                     GGLALISTAPPPTASTAPAFTVRDGWTLADLERHIEAGAVDAITAVPTTGANPGAQRA
                     TPRSSGLATLGGRFVHSDDAAT"
     gene            complement(9302..11029)
                     /locus_tag="VHM48_12190"
     CDS             complement(9302..11029)
                     /locus_tag="VHM48_12190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2756221.1"
                     /translation="MSRNTRVLVLIAAMAAASFTVGGLVYVVTDADAAKVNAAAARQT
                     QIRARATELASAVATEQSALDDFVLSESSLMTERFDAAIEREASTSAKLWKLAGDTPE
                     IVVALASFDAASLEWRAKLANPVIAAIRTGNTAVVDDFRHRSVADHDKVDGALTGLET
                     ALRIADRDLADRTAASNGLKVLGIAIAFGFLLVAFGVALVVVRRFGQTLERDARQASI
                     LNRFTELTTFANADQELAVANLVALGRLVSPDASVTHILNRSLDRAVPEAQTGQAIAE
                     VLPLHALGRCAGVLRGTVYVADDLSDELSVRCPIYPVQSGTLACVPLISGESVGSVHL
                     YWKRPGALPLEFRSNIARITEHAALAIGNRRLLVALHGQANTDARTGLSNNRAFDLAL
                     EAALAGRANHESLSVLMIDIDHFKQFNDRHGHPAGDEALKAFAAVLRSCMRDGDVASR
                     YGGEEFAVFLPGIGQDAAKSIGERIRARIEATIISLAPGLTDRITISIGVSSAPEDAS
                     DRVTLLRLADEALYRAKESGRNRVVAISGEGGAAATDTDDASPPAPPQLARFPRRASA
                     GRASGPVRE"
     gene            complement(11106..12248)
                     /locus_tag="VHM48_12195"
     CDS             complement(11106..12248)
                     /locus_tag="VHM48_12195"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEX2756222.1"
                     /translation="MHVASSNLVSRSISPADDARARIRASAERLRAALARTVTADPAL
                     AAAAADLSAGYRAGREPAGGAGHVHDPRGAAAYAAIRMPATFAAVGRAMAEAALALPA
                     FAPASLLDVGAGTGAAAWAAAAVWPGLVDMVAIDREPAMIELGRRLAAPLRAGEPDAL
                     GNATWRVVEGEAGSFPASDVVAAAYLLGELGDPGDRAALVSRLWEATAGALVLVEPGS
                     RPGFGRILAARSLLIEAGATIAAPCPGNVACPVQEPAWCHFLARLDRSPLHQRAKRAV
                     RSWEDEPFSYVVATRAPSEPRPRIVLGRPRQRPGQVELRICRDGRIETRIVSRREGPA
                     WRIARDLGWGDRLPADLDASRHDARDVAHGDSGDPALRHLDENGHP"
     gene            complement(12212..12287)
                     /locus_tag="VHM48_12200"
     tRNA            complement(12212..12287)
                     /locus_tag="VHM48_12200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12252..12254),aa:Gly,seq:tcc)
     gene            12902..13882
                     /locus_tag="VHM48_12205"
     CDS             12902..13882
                     /locus_tag="VHM48_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2756223.1"
                     /translation="MRAIVCTNYGPPDVLQLRDVLKPVPKDNEVLVRVRATTVSAADC
                     ELRRFDFAPWIWVPMRLAFGIRRPRRRVLGQEFAGDVDAVGKKVRSLRKGDRVFAATG
                     IPLGAYAEYICLRENPQTGAITTMPANLDYAEAAAVPYGGGEALQFLRKANVQSGQRV
                     LVNGAGGSFGTFAVQLAKALGAHVTAVDSAPKLEMLRTIGADRVIDYSQEDVTDGAET
                     YDVIFDVVRSTPSGRMVRLLTETGWLLMANPGFLQIVRAKWASRGSKKRVSFAATGVT
                     IEDLAHLRGLVEAGRLHPVIDRRFPLEQMVEAHRYAESGQKLGNVVVVVA"
BASE COUNT         2192 a         4795 c         5031 g         1904 t
ORIGIN      
        1 gcgccaacat cacccccgag gatgtcggca agaccgcgct ctacctcgcg tcggacctgt
       61 cgtcggccgt gactggcgag gtgatctacg tcgacggcgg gttcagcgtg atgggcgtac
      121 cgagcggcga ctgagggcgg cagggcggcc aaggcggcca agtcggcgcg gcgaagccgg
      181 cgctcgggtc acctaccacc cgccgtagtt gaagtcaggc cgacctcgac gtcatcgcga
      241 gattcgggct gaaaccggcc gaaacgggcg tcacccactc ccccgggtgg taatggcgaa
      301 ccagggctgg atccggcggc cacgcaccac tccccgtggt ggatgacacg acccggagat
      361 cgagcgctac ggatccggca ctggcccggg caatcgtcgt gcgccgcaga gccggattgc
      421 ccatacgcca gccggggtgg cagtggagcg aggatcatct tcatccgctc gcgtgcgcgg
      481 catatgacga agatccaacc aggtcccgtg cgtggcaggg tgggcatgga ggggtctggc
      541 tcgcccgagt gccacctcca gtgacgtatg gtgaaatccg ggggcgacgc gctcaggtcg
      601 cccgggtgaa gtccttcaca atcgaggcgt ggtagacctt cgggcgctcc tcgacgagat
      661 cgctgtcgtc gctgccggcg gcaccgtcga ggtcgaccca cgtgacgtgg gcacggttca
      721 ccaggatcgt ctcgaaatgc tcggtcgtcg gcacgtcggc cttgtcatag gtgatcgtcg
      781 cgtcggtcag cgggaccagg atgtcgcgac cgacgaagac cgcagcgggg ttgcccgcag
      841 ccaggcgatc cgcggccccg atcgggacgt gcagcgatcc cgacacgacg aaccgggtca
      901 gcccgacccg cgcccggtgg acctcggtct tccgccgccg ggaatcgatc ccccgccggc
      961 cctcgacgag gacggcgacg atcgagccga ccgcgagggt gaggtccgcg gtctcgagcg
     1021 ctgcgtcgcc gatcaggtcc tcgatcacag caccccggat cacgatccgc gccacggagt
     1081 tgagcatgtc gctgaggcgg tcgtcggcaa gggggatcgc gccgtggatc cggcggtcgg
     1141 cggtgaaggc gatcaggtcg acgggcatcg tggcggcagc gtgcggcatg ccggcgcgat
     1201 cggacacgac tcgatggtcc tgggacgtga cgccgggccc gactattccg cggcgcggat
     1261 ctcgtagcgg gcgagccgcc aggatgccag cgcggtgatg accacgatcg cgacggcggc
     1321 gaacaggaat ccgttggcgc cgacgagcga gtcgatcgcc ccgcgcggcg ccagcgtcga
     1381 ggccatgccc cggatgtact cgcggacgct gaacagctgg gtccccttga gcgcccccga
     1441 caggagccct tcccacaaca gcgagtagcc gaggccgatg accagcccgc gactcgtcgc
     1501 cgcgctcagg gcgacgaaga tcgcgctgta gacgaacgag ccgatcagga ccgcgatggc
     1561 gaacgcgaac gtcagcccga gctcgcggct gcccaacccg ccgacgagga tcccggcgag
     1621 gatcgtcgcc gggaacagga ggaccgcggt catccccccg gccacgacga acttcggcag
     1681 gacgatcgcc cagcgcggga tcggcttggt gaggacatgg accgcggtcc cgtcgtcgag
     1741 ctcgctgccg agcgccgccg tgccgaagac gagggcgacg agcggcagga cggccgacac
     1801 gaccaggcct tccagcgccg acggcagcgc gcccgagcgc gtgccgccga tccgcacgag
     1861 caacccgagg aggaccggcg ctccggcgag cagcagcatc aggatcgacc gccgccgccc
     1921 gaggagcgag cgggcggtga ccgaggcgat cgtgacgatg acgtcgagga ggttcatcgc
     1981 cgcgtcacca ggtactcgaa gacactctcg agcgagtcgt cggtcggggc gacctcgaag
     2041 aggcggacgc cggcatcgcg cgccacccgg gcgacgatcc gggtgaaccc ggagaactcc
     2101 gacaccgaga cggcgagccg gtcgtcgcgc agctcggcgc cgaagacccc gggctcggcg
     2161 agaaaggccg atgcgagtcg ccgatcgtcc gacgagcgga cccggaacgt gtgcggccgg
     2221 tcggtcatca ggcggcggat ctcgcggtag tcgccggcgg cggcgagccg ccccgcatgg
     2281 atcacgagga tcccgtcggc gagacgctcg acctcctcga ggatgtgcga gctgaacagg
     2341 atcgtccggc cggccgccgc catcgagcgg aggagatcca tcatgtgcag ccgctggcgc
     2401 gggtccatcc cgttgaacgg ctcgtcgagg aggaggatcg acggttcgtg gacgagagcc
     2461 gccgcgatct tgacccgctg ccgcatcccc ttcgaatacg tcccgaccgc ccggtcggcg
     2521 acggacgcca gatcgaccgt ggcgatcgcc cgttccgtgg ccgcggccgg gttcggcagg
     2581 cgttgcagcc gggcggcgag atcgacgaac tggcggccgg tcaggaacga gtggacggcc
     2641 tcgcgctcgg ggacgagccc gatgtcgcgg tagatcgacg gctcgtgcca ggccggccgg
     2701 ccgccgatca gcacccgacc cgacgacggc cggagaaggc ccgccagcat gtgcaacagg
     2761 gtcgacttgc cggcgccgtt cgggccgagc aggccggtga tcccggagcc gaggctgaag
     2821 ctgatgtcgt tgactgcgac gacgttgccg taccagcggg agacgcggtc gaggacgatc
     2881 ggctggttcg ggtcaacgag cgtcggcgac gcgagcggca cggaggacgc tggcgcctgc
     2941 gtcggtgacg tctgcgggcc ggtcatatcg tgatccgggc aaagcggcgc aggatcagcg
     3001 ccacgacgac ggcgacctcg acgatcgccg cgacgaggaa cgcccagtgt gggaggtcga
     3061 cgaagaggta ctccccgccg agcggcccac cgaagacgaa tgcgttcgtt ccgtcgatga
     3121 tcgtgttcgg gctgatcagg acgagtcccg tcccgacggc gctggaaccg atctggatca
     3181 cgattcccgc gatcagcccg ggcaggacga acagggcgat gatccccgcg gtcgcgtacg
     3241 cccggcgcgg ggtgtaagcg gaaattgcca tcgcgagccc gccgtacagc gccgcgatga
     3301 ccgcgccctg ggcgacgacc ggaggcacgc tcggcaggtt cttgccgagg gagcccgcga
     3361 tgtcggtcga gagcaggatc cggccgagga acaggatgat catcgggatc aggagcagga
     3421 cgaggaccgc gagcatgaac ccgaggagcc ggccgagggc gtagtcggat cggcgcaggg
     3481 cccgggcgaa gtacagggcg agcacgccgt gacgctggtc gcgcccgaac agctccggcg
     3541 cctgggccgc gcagaacagg acgacgatcg cggtcaccga gctgtagtag ttgttgaacc
     3601 cgatcagctc ggtgtcgtcg agctggcggt cgaacccgaa ccgggccgcg atcgtcaggc
     3661 cgccgacgat gatcaccgcc ggcaggatcg ccatcgtccc gaagatgaac ggcgcggcct
     3721 ttgcccgtcc gctccggccg atcccgaacg tcgtccggaa gctgtgcacg acgagcgatc
     3781 ggatggcgtg cgcccggccg agacgctggc cctcgtagcg gcggtagccg aggtcgtaga
     3841 tgttgccggc acgggtgacc ggcgggaggg cgctcatcgg ccgaccgccg gcgggagcgt
     3901 ctcggcagag ggctgaacgg cggcggcggc ggccgccgga tcgtcgcgga agaggtcctc
     3961 gagccgatga cggcgctgct ccatccggac gagcgcgagg tcgagctcca cgacggtgtc
     4021 gcggacgatg tcgaacggcg tctcgtcgac gaggtcgacc aggatcgccg ccccgtcgac
     4081 cacgacccgg aggccacgcg ccgtcagccg ttcgacgagc gcctcgcgcc cttcctcgac
     4141 ctcgaccgcg agcgagccgg tccgttgggt gaacgagtgg agcggcgccg agcggaggag
     4201 cttgccgccc tcgatcgcca cgaggtggtc gcagacccgc tcgatctcgc cgagcagatg
     4261 cgaggcgacg atgacggcga tcccgaactc ggtgccggtc cggtggatca gctcgagcat
     4321 ctcgtcgcga ccggccgggt cgagtccgtt cgtcggctcg tcgaggagga ggagcctggg
     4381 gtcgtggacg agcgcttggg cgagcttgac ccgctgcttc atgcctgtcg agtagccgcc
     4441 gatcgcccgg tagcgctcct cgtacagccc gacgtggcgg aggacctcgg ccgtccgctc
     4501 gcgcgccgcg gccttcggca ggcccgagat cagggcgaga tgcgagacga tgtcggtcgc
     4561 ggtcatgtcc ggcggcaggc agtcgtgctc gggcatgtag ccaatcagct cgcggatctc
     4621 gatcccgcgg gtcgccaccg ggaggtcgaa gatcgtcgcg ctgccggagg tgggttcaag
     4681 caggccgagc aggatcttga ggagcgtgct cttgccggcg ccgttcgacc cgaccagacc
     4741 cacgacgccc ggctcgatct cgaccgtcaa gccgtcgagc gcggtgacgc ccccggggta
     4801 gcgcttggtc agggcggtgg tcgcgatcaa gctcacgggc gacagggtac cgtccggtga
     4861 tcgcgggcgt cgcccgtccg cgccggatgc gagatgtatt ggggcccacg ggttcttgaa
     4921 tggagtcgaa ggatcgagcg catcacgatc gcaggcgcga acgcagactc gaaccggccg
     4981 tttcgacgag cggcctctcg ctgtcggaca ggcgcttgcg gtcagtcctg aaccggtcgc
     5041 tacactcgga cgcgatgtcc cccgatgtcg ccgaacggca gctcctcggc gccgagctgt
     5101 gggagcgcgg cgatgcgacg acatcggccc gcttcctggc cggctgggag gacggcgcca
     5161 ccttctgcgc cgcggtctgc tcgaccgacc tgggtctcgc gttctgccgg gcctgcccca
     5221 cggacgttgc caaccgtgcc ctggcgaacc gccgcgcgac ctccggccgc tgcccggccg
     5281 gcgtccgcct gctcgccttt ccggcacctg ccggatcggt cgaccgcgtc gccgtcctgc
     5341 gggtcggacc gccgacggcc aggcaggccg tcgccgtcac cgaccaggtc cgggtcgccc
     5401 cgaccgccct ccgccgggcc gcccgcgaga tggagcccgc cgacgggcga gcgtcgctgt
     5461 cggccgcgcg gcgtctgcgc gaccccgccg gcaaccgcga ctggcaggtc gagcagcggg
     5521 cgcgcggcgc ggatcgccgt cgggcagccg gccaggccct cgcccagatg atcgcaacga
     5581 gcgaggagct ccatgagctc taccgtgcct cgcaacgcca gcgccgtcag ctggaccgca
     5641 atcgccggct cgtcgacggc ctcgcccggg cgaccctccg cgcccaggac gaggaacgga
     5701 gccggatcgc ccacgagatc cacgacacgg ccgcccagtc catggtcagc gccttccgct
     5761 ttctcgatgc ggcccgggca gcgagcggcg cgaatggcca cgagccggac cctcgcctgg
     5821 tccacgccag cgagcgcctc cagagcgcga tccgcgaggt gcgggccgtc ctcgcccggc
     5881 tcctgccgcc gggtctcgag gagctcggac ttgccgacgc cctccggacc cggctcaaca
     5941 cggcccacgc cgacgacggg agccactcaa cgaccggcgc cgtcagcggc gacctgcccc
     6001 gcctcaaggc ctgggtggaa caggccctgt acgggatggc gtcggaggcg gtcagcaatg
     6061 ccgtccgcca cggtcaggcg acgaccatcg ggatcgatct gggcgtcgtc cgcggccggg
     6121 ccgtcgtcac cgtgaccgac gacggcggcg ggttcgatcc gacgacggtg atccggaccg
     6181 agggcagcgg ccttggtctg gtcgggctga cccgccaggc atcatggctg ggaggctcgg
     6241 cgcggatcgt cagccggccc ggtggcggga ccacggtccg gatctcgatc ccgctcgcac
     6301 ggcacctccg cggttcgtcc ggcgacgagg ccggatcgga agccgacgca acggccgtcg
     6361 agtcgcgtcg ctcgggcgag ggccagtgat gagcggtcgc ccgaggcaag caccgaggga
     6421 tgaccggatg accgagcgac gcagggtcgt gctgatcgac gaccacgaga tggcgcgccg
     6481 cggcctcgag tcgatgctct cgaccgcgaa ctggatcgac gtcgtcggcg atgccgacga
     6541 ctgcgccggg ggcctcgctg ccatcgagcg attccgcccc gacatcgtcc tgctggacat
     6601 ccgcatgccc ggcacggacg ggctcgcgtg cctcgaccgg atcaaggcgc gagcggaccc
     6661 ggtcgccgtc gtcatggtca cgctctacga cgaccgccgc tacgtcctcg aggcgatccg
     6721 tcgcggagcg gccggctacc tgctcaagga cgcctcgacc gacgacgtga tcgcgacgct
     6781 ccaacgcgtg gccgacggtc agctcgcgat cgatccacag ctcctccgcg aagccctgac
     6841 cacccgcgac gacctgcccg cccgggcaac cccgcggcaa cgagcggagg cgtacagggt
     6901 gaccccgcgc gagcacgacg tcctcgccct ggtcgccgaa ggcctgacca acaaggagat
     6961 cgggtcgcgg ctgtcgatca gcgaggacac cgtcaagaag cacgtccaga acatcatctg
     7021 gaagctccgc gctgccgacc ggacgcaggc cgcgatcacc gccttccgga tgggcctgct
     7081 cgaggacgcc tgacgatggc cggccgatcc gcgccgggcg cgtcattcat ctgaccggcg
     7141 gatccggacc gccgtcgctg ggcgtacgct ccgcgcatgt ccgatgcgga gaaggtcgtc
     7201 cggcaccgcg cgccgatcat cctggtcgtc gatgacgacg agtacgtcca ctcgacgctc
     7261 gccgcggcca tgcgcagcct gcggccggac atcctgtggg cggcaaccgc cgccgccggc
     7321 ctggccctcg cgctcgaccg gcggcccgac ctggcgatcg tcgatgtcgg cctgccggat
     7381 ctcgatggct acgccctggt tcgggccatc cgcgcccgtc cggagctcgc gggcctgcgg
     7441 gtctgcatcc tgaccggcca cctgcccgac gagaccctcg ctcgcgacgt cggcgcgaac
     7501 gccatcatcg gcaagccgtt ccgtctcgac gagttcctcg ccacgatccg cggacagctc
     7561 cggatccgtc cgggcgacgc agccggtgcc cggcccgtcg tcggctgatc gcatccaggg
     7621 cggccatcga gcgcgcgcgc ctgccgctgc ccggccgagg ccgagcctca ggcgcgggac
     7681 gcggcgcgaa tgttgaagcg ctcgaggagg tcaccgaccg cctggcgggc cgcgcgccac
     7741 tcctcctggt cgccgaagat ctcggcctcg gggccccacc gtttgagctg ggacatcgcg
     7801 gtcacgatcg cggagtgctg ataggaccgt cgccggccgc cgtcgatcgc gccggtgacg
     7861 aggtagcgcc gaaccaggtc gtacggcgcc tgccagcgcc cgatccggat caggtcgccc
     7921 gaccggagcg actcgaggta gtcgtcgtcc ccgacggggt actgctcgtg ctcgaccatg
     7981 tgggccacga ggtcgatcct ggaggtccgc tgacgacagg acgcgatgag gccatcgtcg
     8041 tcgaccgtcg catagcacgt cggacaggac ggcagctcgg cctcggcccg tggcgccgca
     8101 ccgatcggca gtccgcagcg ccggcagaac gagacgtcgg ttcctgcggc gatcatgccg
     8161 cactggaggc agaccgggtc ggtggcctcc cggaccggaa ccgcggtggt ggcctgccga
     8221 cccggagccg cgccggcggc cccgggacga ggcctggacg atccgaagag cttcatcatg
     8281 accgcctcgt gtggtgggcc gacgccatca ggttccagat gatctcgagc gtccggcggg
     8341 cggctgcgat ctgctcaggc tcgagcagca ggagcggcag gaggccgccg gaacggacgg
     8401 tcgcacgaat caccccgacc gggccgtatg cgtcgagacg gacctcgcgc agctgctcgt
     8461 agctccagcg gcggtcgccg tcggcggccc atgccgagga acggctcggg tagaccgtca
     8521 ggcccaggcg ggtcgcccgg aggatcgatc cctcgatccc gtggacctcg aggaacgact
     8581 gctcgacatc gtcggcgtgg atccgggatg actcgatcga cgcgtcgctc tcgtacgtat
     8641 tcatcggtct cctgcaggtg acggcggtgc agatcgttcg atcggaccgg gtgcgccacg
     8701 ttccttccaa cggtgccatc cagagggtcg gatcgatatc ggccgaaggg gtgccggcgc
     8761 ggttcgcgtg cggtcgattg gtcacgtggc cgcgtcatcc gaatggacga accggccgcc
     8821 gagtgttgcc aggcccgagc tccgtggagt cgcccgctgt gctcccgggt tggcgcccgt
     8881 ggtgggtacg gcggtgatgg catcgaccgc gcccgcctcg atgtggcgct cgaggtcggc
     8941 gagcgtccat ccatcacgga cggtgaaggc gggtgccgtg ctggccgtcg gcggcggagc
     9001 cgtgctgatc agggccagcc cgcccgaacc gacgagcgcg acgatgacga tcagcaaccc
     9061 agcggcgaag accgtccgcg gacggcgacg cagcgcgacc cgaagggcgg tggtcaggcg
     9121 acgggtgacg gtcgtcagtc ggcgcggggt gttcatggca tccaggggaa tcggagacct
     9181 cgaaggtccg atcaggcggc ggtccacgcg atcctcgcgg acggggaagt ccggccgaat
     9241 catcggattg ggtgacatcc agcgggtcga tcccggggtg acgtgccgtc cggccgcgtc
     9301 ctcattcacg gacgggaccg gacgcgcggc cggcgctggc ccgtcgcggg aatcgggcga
     9361 gctgtggcgg cgccggtggc gacgcgtcgt cggtgtcagt cgccgctgca ccgccttcgc
     9421 ccgagatcgc gaccacccgg ttccgaccgc tctccttcgc ccgatagagg gcctcgtccg
     9481 cgaggcgcag gagcgtgacc cggtcgctgg catcctcggg agccgagctg acgccgatcg
     9541 agatcgtgat tcgatccgtc aagccggggg cgaggctgat gatggtcgcc tcgatccgcg
     9601 ccctgattcg ctcgccgatc gacttggccg cgtcctgtcc gatgccgggc aggaacaccg
     9661 cgaactcctc gccgccgtag cgcgacgcga cgtcgccgtc gcgcatgcac gaccgcagga
     9721 cggcagcgaa cgccttcagc gcctcgtcgc cggctggatg accgtggcga tcgttgaact
     9781 gcttgaagtg atcgatgtcg atcatcagca cggacagcga ctcgtggttg gcgcggcccg
     9841 cgagggcggc ttcgagggcg aggtcgaagg cccggttgtt cgagagtccc gtccgagcat
     9901 ccgtgttggc ctgtccatgc agcgcgacca ggagtcggcg gttgccgatg gccagggccg
     9961 cgtgttcggt gatccgggcg atgttggacc gaaactccag cggcagtgcc cccggccgct
    10021 tccagtagag atggacggaa ccgacggact cgccgctgat cagcggcacg caggcaaggg
    10081 tgccgctctg gaccggatag atcgggcagc gaacgctgag ctcatctgac aggtcgtcgg
    10141 cgacgtagac cgtcccccgg aggacgcctg cgcagcggcc gagggcgtgc aacggcagga
    10201 cctccgcgat ggcctgaccg gtctgggcct ccggaacggc ccgatccagg gatcgattga
    10261 ggatgtgggt aaccgatgca tcgggcgaca cgagccggcc gagagcgacg aggttggcga
    10321 ccgcgagctc ctggtcagcg ttcgcgaacg tcgtgagctc cgtgaagcgg ttgaggatgc
    10381 tggcctggcg cgcatcgcgt tcgagcgtct gaccgaaccg tcgaacgacg acgagggcga
    10441 ccccgaacgc gaccaggagg aacccgaagg cgatggcgat gccgaggacc ttcagcccat
    10501 tcgaggccgc ggtgcgatcg gccagatctc gatcggcgat gcgcagggcg gtctcgagcc
    10561 cggtcaacgc gccatcgacc ttgtcatggt cggccaccga gcggtgccga aagtcgtcga
    10621 ccacggcggt gttgccggtc ctgatcgcgg cgatcaccgg gttcgcgagc ttcgcccgcc
    10681 attccaggct ggcggcatcg aacgacgcca acgcgaccac gatctcgggc gtgtcgccag
    10741 cgagcttcca cagcttcgcc gaggtcgatg cctcccgctc gatcgcagca tcgaaccgtt
    10801 cggtcatcaa gctgctttcc gacaacacga agtcatcgag ggcggattgc tcggtggcca
    10861 cggccgacgc gagctcggtt gcccgggcgc ggatctgggt ctggcgagcg gccgccgcat
    10921 tgaccttggc ggcatcggcg tcggtcacca cgtacacgag cccgccgacc gtgaaggagg
    10981 cggccgccat cgcggcgatg aggacgagca ctcgggtgtt tctcgacacc gttctggcca
    11041 tgatccgagg atcgcgatcg agccgccgcg cggcacctgg ccgaacgtcc gaagggtcac
    11101 ggcaatcagg ggtggccgtt ctcatccaga tgacgcagag ccggatcccc cgaatcaccg
    11161 tgcgcgacgt cgcgagcgtc gtgacgtgac gcgtcgagat cggcggggag gcgatctccc
    11221 cagccgagat cccgagcgat ccgccacgcc ggaccctccc ggcgactcac gatccgggtc
    11281 tcgatccgac cgtcacgaca gatccgcagc tcgacctggc ccggtcgctg acgtggtcgg
    11341 cccaggacga tccggggacg cggctcggac ggggcccggg tcgcgacgac atacgagaac
    11401 ggctcgtcct cccacgaccg gacggctcgc ttggcacgct ggtggagcgg gctccgatcg
    11461 aggcgggcga ggaagtggca ccacgccggc tcctgaaccg ggcaggcgac gttgcccggg
    11521 cacggcgcgg cgatcgtcgc accggcctcg atcagcaggc tccgggcggc gaggatccgc
    11581 ccgaaccccg gccggctgcc cggttcgacg aggacgaggg cgcccgccgt cgcctcccag
    11641 agccggctca ccagggccgc tcgatcgccg ggatcgccga gctcgccgag caggtaggcg
    11701 gcggcgacca cgtcggaggc cgggaaggac ccggcctcgc cctcgacgac ccgccaggtc
    11761 gcgttgccga gggcgtcggg ttcacccgct cggagcggcg cggcgaggcg ccggccgagc
    11821 tcgatcatcg ccggctcgcg gtcgatcgcg accatgtcga cgagccctgg ccagaccgcc
    11881 gcggccgccc acgcggcggc acccgttcca gcgcccacgt ccaggagcga ggcaggggcg
    11941 aacgcgggca gggccagcgc ggcctcggcc atcgcccggc cgaccgccgc gaacgtggcc
    12001 ggcatccgga tcgccgcgta cgccgcggcg ccgcgcgggt cgtggacgtg gccggctccg
    12061 cctgctggtt cccggccggc tcgatagccg gcggacaggt cggcggctgc ggcggcgagc
    12121 gcgggatcag ccgtgacggt ccgggcgagg gccgcccgaa gccgttccgc cgaagcccgg
    12181 atccgagccc gggcgtcgtc ggcggggctg atggagcggg agacgaggtt cgaactcgcg
    12241 acatgcagct tggaaggctg ccgctctgcc aactgagcta ctcccgcccg aggttcggcg
    12301 aatggaccgc tgattggggt tgacggttag ccgaccccgc tgaggttcct tcagccaacg
    12361 gcgacaacgg accgagacag gtcagccgag gcactcggag cctcgacatg gacacgcccg
    12421 ccgatcccgc gacgtcgttc gccgaagtct accagtcgtt cctcgacgac ctggcgaagg
    12481 agggaccaag ccctcgacga tccactgcta ccgctacaac atcgagcggt tcgagaagtg
    12541 gctcatcgcc aacggctatc cgatcaccct ggcctcgctc gagcgggcga tcctcatcgc
    12601 ctaccgccaa tttctcgaga ccctgcccca gcagccgcgc gggtcgaccc ggcgccggcg
    12661 ggctaatgag ccgcctcgtc agcgtgtagt caggccgatg ctcatgcgtt ggcggggccg
    12721 ttcagctctg gtgccgcggc ggagcttggg tcagcgtccg acgggcagcg ggacgctccg
    12781 acgcggggtc cacgctcacg gtcgccggga ccgcgccgaa ctactggcga gacccaaccg
    12841 ccgcaggtgt cccaggaaag aaacggatcg tgcgtcgcgt cgccattcgt ggtgtcagac
    12901 cgtgagagcc atcgtctgca ccaactacgg accaccggat gttcttcaac tcagagacgt
    12961 gctgaagccc gttccgaagg acaacgaagt gctggttcga gtccgcgcca cgacggtgag
    13021 cgcggccgat tgcgagctgc ggagattcga cttcgccccc tggatctggg tgcccatgcg
    13081 gctggcgttc ggcatccgca gacctagacg acgtgtgctc gggcaggagt tcgccggtga
    13141 cgtcgacgcg gtaggcaaga aggtcaggtc gctcaggaag ggcgaccggg tcttcgcggc
    13201 gacgggcatc cccctgggag cctacgcgga gtacatctgc ctgcgcgaga acccccagac
    13261 cggggcgatc acaacgatgc cggcgaacct ggattacgcg gaggctgcgg ccgttcccta
    13321 cgggggaggt gaggccctgc agttcctgcg gaaggcaaac gtccagagcg gacagcgggt
    13381 cctggtcaac ggggcgggag gaagcttcgg cacgttcgcg gtccagctcg ccaaggccct
    13441 cggggcgcac gtgaccgcgg tggacagcgc gccgaagctg gagatgctgc gcaccatcgg
    13501 tgcggaccgc gtcatcgact actcccagga ggacgtcacc gacggcgccg agacctacga
    13561 cgtcatcttt gacgtcgtcc gcagcacgcc gtccggtcgc atggtgcggt tgctgactga
    13621 gaccggatgg ctcctcatgg ccaatccggg cttcttgcag atcgttcgcg ccaagtgggc
    13681 gtcgaggggg agcaagaagc gggtgtcgtt cgcggcaact ggcgtgacga tcgaggacct
    13741 ggcccacctg cggggcctcg tcgaagcggg aaggctgcac ccggtcatcg accggcgctt
    13801 cccgctcgag cagatggtcg aagcccacag gtatgccgaa agcggccaga agttggggaa
    13861 cgtcgttgtc gtcgtcgcat agcggcggcc gcgatcgcag cctccgggcg ggatgcgcat
    13921 ac
//
LOCUS       DATAIW010000410         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_177203, whole genome shotgun sequence.
ACCESSION   DATAIW010000410 DATAIW010000000
VERSION     DATAIW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177203"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..539)
                     /locus_tag="VHM48_12210"
     CDS             complement(63..539)
                     /locus_tag="VHM48_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2756224.1"
                     /translation="MPDAPVIDAAQRGFLGAARTATLATIGPSGRPRLVPICFVVGAD
                     DSAGRPRLYSALDEKPKSSADPLGLARVRDLLARPEATLLVDRWSEDWDRLGWLRLEV
                     RGELLEPGPGDEAERAAVVTALRATYAQYSTHRLEELPILRFTVERAVSWGDLEAR"
     gene            565..1431
                     /gene="fdhD"
                     /locus_tag="VHM48_12215"
     CDS             565..1431
                     /gene="fdhD"
                     /locus_tag="VHM48_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX2756225.1"
                     /translation="MNHEPPAPPTRHVTTTRIVAVRGQAFEFRADRVVGEEPLEIRAA
                     GPGQDPIAVAITMRTPGFEAELAIGFLRTEGLIEGPEILRTTTGDAASMNQPDDQVTV
                     HLSRPFDASRVAERHFVATASCGICGKASLDEVTVRSAPIPAGPVVSRAVILALPDML
                     RAAQRAFDETGGLHAAGLFTPAGELIALREDVGRHNALDKLVGSQVLAKAMPLHDRIL
                     MVSGRVSFEIVQKAAVAGVPIVCAVSAPSDLAIDASDRLGVTLVGFLRGDGFNVYAHD
                     ERIDLRDLMAFD"
     gene            complement(1436..2458)
                     /locus_tag="VHM48_12220"
     CDS             complement(1436..2458)
                     /locus_tag="VHM48_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2756226.1"
                     /translation="MDRHLLRTTTARLGATIVGATALVEWLLAIARPEGGPLGVLQIL
                     APHLAILGLVLAPVALLGRSRSAVVAAVALAAVIAIRFSGDWMSLPAAAPPAGTDRIA
                     VATWNLEVESRPGVDTATFLRTVTADVIGLQELQPAAAAAIEADPVLRAAFPYRYLQP
                     RTDVLGLGILSRVPILEPSVRLGPAIQEATLDLGGGRRIGVVNAHPFHADIASLGSTR
                     IPVGLDVDQRNADLDTIRARIDARAAGGDPVLLIGDLNTAASEPAFDRFVRGLRDVHA
                     EVGEGTGWTWRPIRLEFLGLGLVRIDHIVVTPGIVPLTTGGTCPPIGDHCLVLAELAI
                     PAGAFG"
     gene            complement(2500..>2718)
                     /locus_tag="VHM48_12225"
     CDS             complement(2500..>2718)
                     /locus_tag="VHM48_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2756227.1"
                     /translation="RDLSRLTRRVLILAGDHDLVRTEHSVAMFEALPDAELAIVPGAG
                     HQVPQTHPAEVAAIVVRFLDEDEAPPGR"
BASE COUNT          396 a          963 c         1004 g          355 t
ORIGIN      
        1 tgagcttcct cgtaccaggc gacgctcgcc tgggcgcggg aatccacccg ttcagcggcg
       61 gctcacctcg cctcgaggtc gccccacgag acggcccgct cgaccgtgaa ccgcaggatc
      121 ggcagctcct cgagacgatg cgtggagtat tgcgcgtacg tggcccgcag cgcggtcacg
      181 accgctgcgc gttcggcttc atcacccggt ccaggctcca gcagctcccc gcggacctcc
      241 agccggagcc agccgagacg atcccagtcc tccgaccagc gatcgacgag gagcgtcgcc
      301 tcgggccggg cgaggaggtc gcggacgcgg gcgagcccga gcgggtcggc gctcgacttc
      361 ggcttctcgt cgagcgcgct gtagaggcga ggacggccgg cggaatcgtc cgcgccgacg
      421 acgaagcaga tcgggacgag ccgcggccgg ccggacgggc cgatcgttgc gagggtcgcc
      481 gtccgggcgg cgccaagaaa ccctcgctgc gccgcgtcga tgaccggagc gtctggcatg
      541 ggtcgatcat gcaacactgc ggtggtgaac cacgagcccc cagcaccccc gacccgccac
      601 gtcacgacca cccggatcgt ggcggtgcga ggccaggcct ttgagttccg ggcggatcgc
      661 gtcgtcggcg aggagccact cgagatccgc gctgcgggcc ccggccagga cccgatcgcg
      721 gtcgcgatca cgatgcggac gccgggcttc gaggcggagc tggcgatcgg gttcctgcgg
      781 acggagggat tgatcgaggg tcccgagatc ctccggacga cgaccggtga cgcggcgtcg
      841 atgaaccagc ccgacgacca ggtcaccgtc cacctcagcc ggccgttcga cgcctcacgg
      901 gtcgccgaac gccacttcgt cgcgactgcc tcgtgcggca tctgcggcaa ggcgtcgctc
      961 gacgaggtca cggtccggtc ggccccgatc cctgccggac cggtcgtctc gcgcgcggtg
     1021 atcctcgccc tgccggacat gctccgggcc gcccagcgcg cattcgacga gaccggcggc
     1081 ctccacgccg ccggcctgtt cacgccggcc ggcgagctga tcgccctgcg cgaggacgtc
     1141 ggccgccaca acgcgctcga caagctggtc ggctcgcagg tgctcgcgaa ggcgatgccg
     1201 ctccacgatc ggatcctgat ggtcagcggc cgcgtcagct tcgagatcgt ccagaaggcc
     1261 gcggtcgcgg gtgtgccgat cgtatgcgcg gtgtcggcgc cgtcggatct cgcgatcgac
     1321 gcgtccgacc ggctcggcgt gaccctcgtc ggcttcctcc gcggcgacgg cttcaacgtc
     1381 tatgcccatg acgagcggat cgacctgcgc gacctgatgg cgttcgactg aagggtcagc
     1441 cgaaggcgcc cgccgggatc gccagctcgg ccaggaccag gcaatggtcc ccgatcggcg
     1501 ggcaggtccc gcccgtcgtg agcggcacga tcccgggcgt cacgacgatg tggtcgatcc
     1561 ggaccaaccc cagcccgagg aactcgaggc ggatcggccg ccacgtccag ccggtgccct
     1621 caccgacctc ggcatggacg tcgcgcaggc cgcggacgaa ccggtcgaac gctggctcgg
     1681 acgccgcggt gttcaaatcg ccgatcagga gcaccggatc gcctccggcg gcacgggcat
     1741 cgatccgggc gcggatcgtg tcgagatcgg cgttgcgctg gtcgacatcg aggccgacgg
     1801 ggatccgtgt cgacccgagc gaggcgatgt cggcgtggaa cggatgggcg ttcacgacac
     1861 cgatccgccg gccgccgccg agatcgaggg tcgcctcctg gatcgcgggg ccgaggcgga
     1921 cgctcggctc gaggatcgga acgcggctca ggatcccgag tccgaggacg tcggtccgcg
     1981 gctggaggta gcggtacggg aacgcggcgc gcaggaccgg gtcggcctcg atcgccgcgg
     2041 cggccgcggg ctggagctcc tggaggccga tcacgtcggc cgtcacggtg cgcaggaatg
     2101 tcgcggtgtc gacccccggt cgggactcga cctcgaggtt ccacgtcgcg acggcgatcc
     2161 ggtccgtgcc ggccggcgga gcggccgccg ggagtgacat ccagtcgccg ctgaaccgga
     2221 tcgcgatcac ggccgcgagc gcaacggccg cgacgaccgc cgaccgactg cggccgagca
     2281 gggcgactgg cgcgagcacc agcccgagga tcgcgagatg cggcgccagg atctggagga
     2341 ccccgagtgg gccgccctcc ggccgcgcga tggccagcag ccactcgaca agggcagtcg
     2401 cgccgacgat cgtcgctccg aggcgggcgg tcgtggtgcg caggaggtga cggtccatcc
     2461 gtggcgagac gatccggggt tgcgccgggt gacggacggt cagcggcccg gtggcgcctc
     2521 gtcctcgtcg aggaagcgca cgacgatcgc ggccacctct gccgggtgcg tctgcgggac
     2581 ctggtggccg gcgcccggga cgatcgcgag ctcggcgtcc gggagcgcct cgaacatcgc
     2641 caccgagtgt tccgtccgga cgaggtcgtg gtcgccggcg aggatcagga cccgccgcgt
     2701 caggcgcgac agatcgcg
//
LOCUS       DATAIW010000411         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_117547, whole genome shotgun sequence.
ACCESSION   DATAIW010000411 DATAIW010000000
VERSION     DATAIW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117547"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..338)
                     /locus_tag="VHM48_12230"
     CDS             complement(174..338)
                     /locus_tag="VHM48_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756228.1"
                     /translation="MSLVPQIIFSGSGIIVAAIGFYVRKDPRRGRATPFLMYLGVLLV
                     GLAVLVTLIR"
     gene            complement(402..668)
                     /locus_tag="VHM48_12235"
     CDS             complement(402..668)
                     /locus_tag="VHM48_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756229.1"
                     /translation="MDTNRVQPGDFYEDCRYHPVICIEADYSEEGDELRGISLVDGGI
                     GSCSPRHCGPVKMSAAETIERRVNWDQFSHVEIPRLTARLDQPN"
     gene            complement(944..1336)
                     /gene="rplL"
                     /locus_tag="VHM48_12240"
     CDS             complement(944..1336)
                     /gene="rplL"
                     /locus_tag="VHM48_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX2756230.1"
                     /translation="MAVLTQDDLLEAIDKMTVLELSEFIKRFEERYGVTAAAPVAAAA
                     APAAGGGGEAAAAPEEQTEFTATLTEIGPNKIPVIKVVRELTALGLKEAKDLVDATPK
                     AVKEGVTRDEAEKIKAAIEEVGGKVEIK"
     gene            complement(1419..1994)
                     /gene="rplJ"
                     /locus_tag="VHM48_12245"
     CDS             complement(1419..1994)
                     /gene="rplJ"
                     /locus_tag="VHM48_12245"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2756231.1"
                     /translation="MRRRRAPAVRQKLEGSMPTEAKRETISELREALAGSRTLIVSEY
                     RGLTVKEIAEIRRALRKQDVSYRVVKNRLMRIAAKDSVGEALSPLLVGPTAIAFGNDE
                     SATAKAVLDATRAYKLVKVTGGVLGDRSIDAEGVKTLASLPSREVLLAKLAGGMQAPV
                     ATLAGLLVANIRNLGYALSQVRDQKAQAEGA"
     gene            complement(2288..2992)
                     /gene="rplA"
                     /locus_tag="VHM48_12250"
     CDS             complement(2288..2992)
                     /gene="rplA"
                     /locus_tag="VHM48_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2756232.1"
                     /translation="MAHGKKYLEAAKLVDRDRIYTPQEAAVLAKETAFVSFDSTLEAH
                     LRLGVDPRHADQMVRGTVVLPHGTGKVVRVVVFAQGDKAQEALRAGADEVGGEDLVKK
                     IESGWLEFDVALATPDSMGMVGKLGKILGRRGLMPNPKAGTITFDLDRAVREVKGGRV
                     EFKVDKSGIIHTAFGRSSFEAEALIANLAALIDAINRAKPAGAKGQYLKGLTIATTMG
                     PGIRVDLPAVLAAAAA"
     gene            complement(3171..3593)
                     /gene="rplK"
                     /locus_tag="VHM48_12255"
     CDS             complement(3171..3593)
                     /gene="rplK"
                     /locus_tag="VHM48_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2756233.1"
                     /translation="MAKKIRIVLTLQLNAGKATPAPPVGTALGPHGINIVEFTKSYNE
                     KTADKTGQVIPAQITIFEDRSFTFILKTPPAADLLRKAAGIDKGAGATKKETAGRVSR
                     DQVRQIAEIKMADLNANSIEAAMLQIEGTARSMGIEVK"
     gene            complement(3749..4318)
                     /gene="nusG"
                     /locus_tag="VHM48_12260"
     CDS             complement(3749..4318)
                     /gene="nusG"
                     /locus_tag="VHM48_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2756234.1"
                     /translation="MTTTETSKAPEKRWYVIHTYSGYENKVKANLEHRIESMGMEDRI
                     FQVLVPMEDEIEIRQGQRHTVQKKVYPGYVLVEMILDPDSWFVVRNTPGVTSFVGGAN
                     IPGVRSEPIPLDENEVKQILRHMGVETPKFRVAFTKGQSVRVTDGPFAEFIGTVDEVN
                     PERNKVKVLVSIFGRETPVELDFLQVEKL"
     gene            complement(4339..4524)
                     /gene="secE"
                     /locus_tag="VHM48_12265"
     CDS             complement(4339..4524)
                     /gene="secE"
                     /locus_tag="VHM48_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2756235.1"
                     /translation="MSRLRRFIDEAWSELKKVSWPTREQVRNLTVLVFVVSFATGAYI
                     TVLDSIFTQVVALLVRG"
     gene            complement(4615..4690)
                     /locus_tag="VHM48_12270"
     tRNA            complement(4615..4690)
                     /locus_tag="VHM48_12270"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4655..4657),aa:Trp,seq:cca)
     gene            complement(4703..4867)
                     /gene="rpmG"
                     /locus_tag="VHM48_12275"
     CDS             complement(4703..4867)
                     /gene="rpmG"
                     /locus_tag="VHM48_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2756236.1"
                     /translation="MARVEARPAIQLACTECKERTYTTRKNKKNDPNRIELMKYCPRE
                     RRHTLHREAK"
     gene            complement(4878..>5076)
                     /gene="tuf"
                     /locus_tag="VHM48_12280"
     CDS             complement(4878..>5076)
                     /gene="tuf"
                     /locus_tag="VHM48_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX2756237.1"
                     /translation="QFYLRTTDVTGAIGLPDGAEMIMPGDNVEMSVELITPIALEIGQ
                     RFAIREGGRTVGAGAIVTIAE"
BASE COUNT          764 a         1657 c         1710 g          945 t
ORIGIN      
        1 gtacgcgcca gcgccacagc cgtccggttc gacgactcag cagacagcat gaaacaccac
       61 tccttgacgg aggcttggag aaaaggaaag ggccgcgctc acagaaggag taggataccg
      121 cgatttcgtc gagtgtcaaa cgaggccgac gccgggcagt gtctcagttt ggatcagcgg
      181 atcagcgtta ccagtactgc cagcccgacg agaagaacgc cgagatacat caggaacgga
      241 gtcgcgcgac ctcgacgcgg gtccttgcgg acgtagaacc cgatcgcggc aacgatgatg
      301 ccggagccgg agaagatgat ctgcggaacg aggctcatcc cccggagtct agcccccggt
      361 cccgcgcgct cgggcgcacc ggcttcttcc ggggcagggt gtcagtttgg ctgatcgaga
      421 cgggcagtaa gcctcgggat ttccacgtgg gagaactgat cccagttcac tcttcgctcg
      481 atggtctcgg cagcggacat cttcactgga ccacaatgcc gtgggctgca cgaaccgatg
      541 ccgccatcaa ccagcgaaat cccgcgtagc tcgtcgccct cttccgagta gtcggcttcg
      601 atgcagatca ccgggtgata ccggcagtcc tcatagaagt cgcctggctg cacgcgattc
      661 gtgtccatcc gcggagtcta gcctcgggac cagttcgctt gactcactgg tcgagtcaga
      721 atcgtcggca gcgcgctcgg tcgcgacgga tctgccacac agccccgaat gctgattgcc
      781 gtcctgactc aaccgatgag tcggacccac cgaccgcggt ggttcccgcg ccgggaccgg
      841 cgtcaccgag gcggcatgag aacaggaaag ccggcaaccc aatttggatc gccggcctcg
      901 aaacggtcgt cgcgcgccgt cacagtgacg gcagattgcc gacctacttg atctcgacct
      961 tgccgccgac ctcctcgatc gcggccttga tcttctcggc ctcgtcgcgg gtcacgcctt
     1021 ccttgacggc cttcggggtc gcatcgacga ggtccttggc ctccttgagg ccgagggcgg
     1081 tcagctcgcg gacgaccttg atgaccggga tcttgttcgg gccgatctcg gtcagggtcg
     1141 cggtgaactc ggtctgctcc tcgggagccg cagcggcctc gccgccaccg cccgcggccg
     1201 gggctgccgc ggccgcgacc ggagccgctg ccgtcacgcc gtaacgctcc tcgaagcgct
     1261 tgatgaactc ggacagctcg aggacggtca tcttgtcgat ggcttcgagc aggtcgtcct
     1321 gggtcaggac cgccatgtca ggtctccttg tcgggtgcgc cggtcatcga ccgatcgcga
     1381 tgtcgaatgg ttcgggtggt cgtcgtcggg aacgcggatc acgcgccctc ggcctgggcc
     1441 ttctgatcgc ggacctggct cagtgcgtag ccgaggttcc ggatgttcgc gacgagcagg
     1501 ccggccagcg tggccaccgg tgcctgcatt ccgcctgcga gcttggcgag gagaacctcg
     1561 cgcgacggca ggctggccag cgtcttgacg ccttcggcgt cgatcgaccg gtcgccgagc
     1621 acgccgccgg tgaccttcac cagcttgtac gcccgggtgg cgtcgaggac ggccttggcc
     1681 gtcgccgact cgtcgttgcc gaaggcgatc gcggtcgggc cgacgaggag ggggctgagg
     1741 gcctccccca cggagtcctt ggcggcgatc cgcatcaggc ggttcttgac gacgcggtag
     1801 ctgacgtcct gcttgcgcag ggcgcgacgg atctccgcga tctccttgac ggtgaggccg
     1861 cggtattcgg agacgatcag cgtccggctg ccggcaagcg cctcacgcag ctcggagatg
     1921 gtctcccgtt tggcctctgt gggcatcgag ccctccagct tctggcgcac ggccggtgcg
     1981 cggcggcggc gcatcgcggt ccttgaaacc acgacacccc tgcgacggcg cctgttcggc
     2041 tggccttgca cgcaggggcg cggccaccgc atggtggcga cacgtgtcct gcctcggcgt
     2101 gctcaccgtc gtccccgatc gccttgcggc agatcgatcc gatccggtgg atcaagcccc
     2161 gaccgtcacg ctccgtgaag cgtcgcggtc cgcgggcgca ggctgtctct ggctatcccg
     2221 tcgatcttca gttgtggcga cgcgtcgggc ccgagggcgc ggccgtcacc gcgtatggtg
     2281 gcctccgtca ggcggcggct gcggcgagga ccgccggcag gtcgacccgg atccccgggc
     2341 ccatcgtcgt cgcgatcgtc aggcccttga ggtactggcc cttcgcgccg gccggcttcg
     2401 cccggttgat cgcgtcgatg agcgccgcga ggttcgcgat gagcgcctcg gcctcgaagc
     2461 tcgatcggcc gaacgccgtg tggatgatgc cggacttgtc gaccttgaac tcgacgcggc
     2521 cgcccttgac ctcgcgcacg gcccgatcga gatcgaacgt gatcgtgccg gccttggggt
     2581 tcggcatcag gccgcgccgg ccgaggatct tgccgagctt gccgaccatg cccatcgagt
     2641 cgggtgtcgc cagcgcgacg tcgaactcga gccagcccga ctcgatcttc ttgaccaggt
     2701 cttcgccacc gacctcgtcg gcgccggcgc gaagggcctc ctgggccttg tcgccctggg
     2761 cgaagacgac gacccggacc accttgccgg tgccgtgggg aaggacgacg gtgccgcgga
     2821 ccatctggtc ggcgtggcgg ggatcgacgc cgaggcggag atgggcctcg agggtcgagt
     2881 cgaaggagac gaacgcggtc tccttggcaa ggaccgccgc ttcctgcggg gtatagatcc
     2941 ggtcgcggtc gacgagcttc gccgcctcga ggtacttctt gccgtgtgcc attcgatgtt
     3001 cctttcgtcg gccgggcgta ccagcctccc cctgtcgttg aaccgccgcg gccggggtga
     3061 gccgcgagtc cggaatgctg gcctcaggcc gatggcgtcg cggagctccg cctcggactt
     3121 cggcgagcga gagtctaccc gacgctcagg gtgccagctc cgagtcggcc ctacttgacc
     3181 tcgatcccca tggatcttgc ggtgccttcg atctggagca tggcggcctc gatgctgttg
     3241 gcattgaggt cggccatctt gatctccgcg atctggcgga cctggtcgcg tgacacgcgg
     3301 ccggccgttt ccttcttggt cgccccggcg cccttgtcga tcccggcggc cttgcgcagg
     3361 aggtctgcgg cgggcggcgt cttgaggatg aacgtgaacg aacgatcctc gaagatcgtg
     3421 atctgggccg ggatgacctg gcccgtcttg tcggcagtct tctcgttgta cgacttggtg
     3481 aactcgacga tattgatgcc gtgcggaccc agggcggtgc cgaccggcgg cgcgggggtc
     3541 gccttgccag cattcagctg aagggtcagg acgatgcgga tcttcttggc cacggggagg
     3601 acggttcctt tctagcgggc gtcagcccgg tgagcgccaa gccgggcggt ccaggccaag
     3661 gcggaggcga gcacgcgagc gagcgcacct tggtgcgtga gcgagcgcgc ggagccgaca
     3721 acgccggcat gggccgtccg ggtggtggtc acagcttctc gacttgcagg aagtcgagct
     3781 cgaccggggt ctcgcggccg aagatgctca cgaggacctt gaccttgttg cgctccggat
     3841 tgacctcgtc gaccgtgccg atgaactcgg cgaacggccc gtcggtgacc cggacgctct
     3901 ggcccttcgt gaacgcgacc cggaacttgg gcgtctcgac ccccatgtgc cggaggatct
     3961 gcttgacctc gttctcgtcg agggggatcg gctcgctgcg gacgcccggg atattggcgc
     4021 caccgacgaa gctcgtcacg cccggggtgt tgcggacgac gaaccaactg tcggggtcga
     4081 ggatcatctc gacgaggacg tagcccgggt acactttctt ctggacggtg tggcgctggc
     4141 cttggcggat ctcgatctcg tcttccatcg ggacgagcac ctggaagatc cggtcttcca
     4201 tgcccatcga ctcgatccgg tgctcgaggt tggccttgac cttgttctcg tagcccgagt
     4261 acgtgtggat gacgtaccag cgcttctccg gcgccttgct tgtctcggtg gtggtcatct
     4321 cggtcatgat cgtgtccgtc accctctgac caacagcgcg acgacctggg taaagatgga
     4381 gtccaggacg gtgatgtacg caccggtggc aaagctgacc acgaagacga gcacggtcag
     4441 gttgcgaacc tgctcccgcg tcggccacga gaccttcttc aattcggacc acgcttcatc
     4501 aatgaagcgg cgaagtcgac tcacgtcggt ggttcttctt tcagctacgt tctaccaggt
     4561 tgggctctcc gagggcggag gccgcagtcc tcagggccat ccgcccgagg aggatggcag
     4621 gggcgatagg actcgaacct acaaccgctg gttttggaga ccagtgctct gccaattgag
     4681 ctacacccct gcgaatgccc cgctatttcg cctcgcgatg aagcgtatgg cggcgctccc
     4741 ggggacagta cttcatcagc tcgatccggt tcggatcgtt cttcttgttc ttgcgcgtcg
     4801 tgtaggtccg ctccttgcac tcggtgcagg cgagctggat cgcgggccgc gcttcgactc
     4861 gtgccatggt cggatcctta ctcggcgatc gtgacgatcg cgccggcgcc gacggtccgg
     4921 ccaccctcgc ggatcgcgaa gcgctggccg atctcgaggg cgatcggggt gatcagctcg
     4981 acgctcatct cgacgttgtc gccgggcatg atcatctcgg ccccgtcggg caggccgatc
     5041 gccccggtca cgtcggtcgt gcgcaggtag aactgc
//
LOCUS       DATAIW010000412         8167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_220871, whole genome shotgun sequence.
ACCESSION   DATAIW010000412 DATAIW010000000
VERSION     DATAIW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220871"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1099)
                     /gene="amrS"
                     /locus_tag="VHM48_12285"
     CDS             complement(38..1099)
                     /gene="amrS"
                     /locus_tag="VHM48_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX2756238.1"
                     /translation="MSMIDDPPRQPAGDAVPSLLATAQPDGAVRCGVCAHRCLVRVGR
                     VGICGVRENRDGVLYSTAYGGAVAIAMDPIEKKPLFHVAPGTQAYSLATAGCPFHCVF
                     CQNWEIAQGPRLGMRPTARPLPPEQAVGEALRLGARSIAYTYVEPTVFLEYALAIARP
                     ARAAGLLNLFITDGYATAESIGLLATVLDAANVDLKSFDDAFYRRRCGARLAHVLEAL
                     EGYRRAGVWLEVTTLIIPGENDDPGELRELTRWLVDHLGAETPWHVSRFFPAFRMLDH
                     PPTSLASLRRAAEIGREAGLRHVYVGNAPELAMEDTHCPSCDTVLIARSGCRVVCRLG
                     DDGTCPTCERAAARPVPRG"
     gene            1341..1937
                     /gene="amrA"
                     /locus_tag="VHM48_12290"
     CDS             1341..1937
                     /gene="amrA"
                     /locus_tag="VHM48_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX2756239.1"
                     /translation="MPFGAPPPPDLRPEAGLILLRLARAVVGAAASRRAASIDLSAVL
                     PPDLPPCLVSPAAAFVTLHRGNELRGCVGSLDAGRALWRNVASAAVSAAHDPRLVPVT
                     ESEVPALSIDVSALGPVVPLADPTAFQPGLDGLIVERDGWRGLLLPEVATEHGWDVVD
                     MLEGTCWKAGLPADAWRDEHTQVSAFRTARVSENGSMA"
     gene            2080..3513
                     /locus_tag="VHM48_12295"
     CDS             2080..3513
                     /locus_tag="VHM48_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX2756240.1"
                     /translation="MDRLDEGLLAELLARGVSRRSFLKLCVTMTGVLALPAAYAPRVA
                     AAVAAAPRMPVIWLRGQGCGGDTEALLRGANPTTAELLLDLLSVDYHDAFMVSSGTAA
                     ATSRVETQARFPNGYFAVVEGAIPTAEDGAYLTIGGRPFGDIVREVCDGAVATIAVGS
                     CAFDGGLAAANGGATGAVGVGRVVSDARLVTLPGCPINVENLTATILHYLTFQALPAT
                     DGRGRPLFAYGALIHNQCGRRSHFEFGEFVQAWGDEGAQKGWCLYKMGCKGPETFANC
                     PSVRYGSGTSWPVKAGAACIGCTTSGFWDAMSPFSRRLPSPIPFAPDVSVDQVGQALV
                     GGVGVLVAAHGTASIARSRLGRKHGVFGAAGEAESFATVEAAPAAQVATEPEPSMLLA
                     AEPIDASVAEPAPPVEPPAPEPAEPVDAGPVEPAVTDVTDVTEAAEAADATDAPGATV
                     DPSPTVDAAGAALDDASAVDPTRTDGQ"
     gene            3515..5224
                     /locus_tag="VHM48_12300"
     CDS             3515..5224
                     /locus_tag="VHM48_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2756241.1"
                     /translation="MTRLVIDPVSRVGGHLRVEVELTDRTVSDAWTSGTMYRGIERVL
                     EGRDARDAWLYAQRVCGTCGIAHAIASARAVENALGLKIPRNARLLRNLMAGVQLVVD
                     HAAGFYLSQALDWVDLASALDADPASTSTLARTLSDWPSSSPSYFTAVQQRLATLLKS
                     GQPGPFAHADWGHPAYALAPDANLMVAAHYLEALDWHRNMMRIQVILGGKSPHPQTFL
                     VGGMALAAAWGGPMRPSPGEHQWDLETTSPPALSANGLADVGRLLDQAKAFVEQVYVP
                     DVTAILKAYGGQSPTDAGIGHYLSFGDFPEDDSDRPALLLPRGRVMGGDLSGAVPVSD
                     TGVGESVDHSWYTTGADDGALVHPRDERTEPHYAGPRPPYAALDGFDRYSWVKAPRYE
                     DDPMEVGPLARLLVAVASAGDAASALSRAVAEVGLGPEALFSGLGRTGARAIESMFVA
                     KRLSAWLADLKENMAAGDLAMADISTWDVETWPAEAAGWAIGESARGAVGHWLTIRNH
                     RIERYQIVDATTWNGSPRDRRGRRGAIEQALIGTRVVDPDRPLEILRTVRSFDPCLTC
                     AVH"
     gene            5238..6002
                     /gene="cybH"
                     /locus_tag="VHM48_12305"
     CDS             5238..6002
                     /gene="cybH"
                     /locus_tag="VHM48_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HEX2756242.1"
                     /translation="MTATPLLQERPAGPAAMEAAPAGHGEERVRLYVWQRPVRITHWV
                     TAGCIAVLALTGGYIADPFLIPPGGSVMTTMRFIHIVAAFTLLASGLIRTVWLLVGNR
                     FARWNAFIPTTRFQATEVFRQAGFYAFLRKEIPKVIGHNQLAAVAYLVLFVLLVVETV
                     TGMTLDGAAGAEPARTMFGWLGEAFGLQWIRLVHHLAMWAILAIALFHVYSCVLIDNI
                     EKNGLLSSMVGGFKFPTRDEVLEARDGGPELLETLE"
     gene            5999..6787
                     /locus_tag="VHM48_12310"
     CDS             5999..6787
                     /locus_tag="VHM48_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756243.1"
                     /translation="MRRPRLPSRLFVERAIRLLVVANVAALGVFAVAVILGHSGTPGS
                     QPPQIAPRPSASFELIAMGNAHIPTTNACILCHGAGGQVKTVPAILHPIEGWRRCTTC
                     HQDENLGRAAPGHDGIAEEECLNCHKTAQTGPAITQPHAALHDQQCLACHGSVAHLPA
                     TMASSREADCVLCHRAATLPPPSYPHPPDARLSCRTCHRSAEAGALPIDHALRTDTTC
                     LLCHDIAIAAAGSPAPGATPAASLLLVPGASSLPLSSPSTAPPP"
     gene            6804..7727
                     /locus_tag="VHM48_12315"
     CDS             6804..7727
                     /locus_tag="VHM48_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2756244.1"
                     /translation="MIRSLLLWASRNRWLREHLSRMRFVRRAVRRFMPGEELTDALAA
                     AESYRSDGIGSVFTLLGENLTTIADADRVAAHYHDVIDEIAKRGLDGEISVKPTQLGL
                     DLDPEVTFAHVDALARHAATAGTWVWVDMEGSAYTDATVALYERAASAHRNVGLCVQA
                     YLRRTPDDLRRLFTVTPSIRLVKGAYDEPATIAWRSARGVDAAFASVATLLLEAQASG
                     RAARVVLATHDTNLIAQELSIGAALGLARDRIEVTMLYGIRSGEQRRLAKEGVATRGL
                     IAYGDYWYPWYLRRLAERPANVIFALRALLP"
     gene            complement(7746..>8167)
                     /locus_tag="VHM48_12320"
     CDS             complement(7746..>8167)
                     /locus_tag="VHM48_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756245.1"
                     /translation="PGTTRSMDLLNVVYEVLLQLLSRYFAHTDETPEQLGVLADASVG
                     LMYAAIKPLGSVVTTLPIGMDVPGVTAGPAFELFYQVDYLLPHRAAAWILMEERLRDA
                     AAFAVRCGQACTPALMEPLAKVARSLEKYADMLAAAA"
BASE COUNT         1131 a         3007 c         2866 g         1163 t
ORIGIN      
        1 cgccggatcg gcgcgacctg ggtgtcgatc ggatcgctca tccgcgcgga acgggccggg
       61 cggccgcgcg ctcgcaggtc ggacacgttc cgtcgtcgcc gaggcggcaa acgacgcggc
      121 agccgctccg tgcgatgagc accgtgtcgc agcttgggca gtgggtgtcc tccatcgcca
      181 gctccggtgc gttcccgaca tagacgtgac ggagtcccgc ctcccgaccg atctcggcgg
      241 cgcgccggag tgaggccagc gacgtcggcg ggtggtcgag catccggaac gccgggaaga
      301 accggctgac atgccacggg gtctccgcgc cgaggtggtc gacgagccat cgggtgagct
      361 cgcggagctc gccagggtcg tcgttctcgc ccgggatgat cagcgtcgtc acctccagcc
      421 acacgccggc ccggcgatag ccctcgaggg cctcgaggac gtgggccagg cgcgctccgc
      481 agcggcggcg atagaaggcg tcgtcgaagg acttcaggtc gacgttcgcc gcgtcgagga
      541 ccgttgcgag gaggccgatc gactccgccg tcgcgtagcc gtcggtgatg aacaggttga
      601 ggagccccgc tgcgcgggcc ggccgggcga tcgccagcgc gtactccagg aagaccgtcg
      661 gctccacgta ggtgtacgcg atcgagcggg cgccgagccg gagcgcctcg ccgaccgcct
      721 gctccggcgg aagcggccgg gccgtcggcc gcatcccgag gcggggtccc tgggcgatct
      781 cccagttctg gcagaagacg caatggaacg ggcagcctgc ggtcgcgagc gagtacgcct
      841 gcgttcccgg cgcaacgtgg aacagcggct tcttctcgat cggatccatg gcgatcgcga
      901 ccgcgccacc gtacgcggtc gaatagagca cgccgtcgcg gttctcgcgg acgccgcaga
      961 tgccgacgcg gccgacccgc acgagacagc ggtgggcgca gacgccgcag cgcacggcac
     1021 cgtccggctg cgccgtcgcg agcagcgacg gaacggcgtc gccggcgggc tgtcgtggcg
     1081 ggtcgtcgat catggacatc ccgatcccca actctcggtc cgagtctggc gcgtgggccc
     1141 cggctcgacg agtgcgaacc ggcccatcgt gacgtggact ttcggcactg ggccgggccg
     1201 gtttcgcggg gcatgctcgg cggagtcacg agggccgccg cccgagggtt gccatggccg
     1261 ccacgaggtc atcgccaaga gtcggggcat cccgaccgga ccgtcctgcc gtccgcgggc
     1321 cggccggcgc gcgcgtcggc gtgccgtttg gcgcgccacc gccgcccgac ctcaggcccg
     1381 aggccggcct gatcctgctc cgcctcgcac gtgccgtcgt cggcgcggcg gcgtcccgtc
     1441 gggcagcgtc gatcgatctc tcggcggtcc tgccacccga tctgcccccc tgcctcgttt
     1501 cgcccgccgc ggcgttcgtg acccttcatc ggggcaacga gctccgcggt tgcgtcggtt
     1561 cgctcgacgc cggccgggcg ctgtggagga acgtggcgtc ggccgccgtc agtgcggcac
     1621 acgatccgcg cctggtcccg gtcaccgaat ccgaggttcc cgcgctctcg atcgatgtct
     1681 cggccctcgg cccggtcgtt ccgctcgcgg acccgacggc attccagccc ggcctcgacg
     1741 gtctcatcgt ggagcgggac gggtggcgag gcctgctcct gcccgaggtc gcaacggagc
     1801 acgggtggga tgttgtcgac atgctcgagg ggacgtgctg gaaggcaggg ctcccggccg
     1861 acgcctggcg cgacgaacac acccaggtca gcgcattccg gacggcccga gtcagcgaga
     1921 acgggtcgat ggcctgacgc tcgcgttcga tcgggggcgg gcgagcgcgc gagggggccg
     1981 gatggcccat cacgagtcac cgaccggacc cttgcggaac acggcccgcg gtcgcaacca
     2041 tggcggatgg aagggctatc gcgctgaccg aaggaccgaa tggatcggct cgacgaagga
     2101 ctgctggccg agctgttggc tcggggtgtg tcgcgccgca gcttcctcaa gttgtgcgtc
     2161 acgatgaccg gcgtgctggc cctgcccgcg gcgtatgcgc cacgggtcgc cgcggccgtc
     2221 gccgccgctc cccggatgcc ggtgatctgg ctccggggcc agggctgcgg gggcgacacc
     2281 gaagcattgc tccgcggcgc caacccgacg accgccgagc tcctcctcga tctcctgtcg
     2341 gtcgactacc acgacgcgtt catggtgtcg tcagggaccg ctgccgccac gtcacgggtg
     2401 gagacgcagg cgcgcttccc gaacggctat ttcgcggtcg tggagggggc catcccgacg
     2461 gccgaggacg gcgcctacct cacgatcggc ggccggccgt tcggtgacat cgtgcgcgag
     2521 gtctgcgatg gggcggtcgc gacgatcgcg gtcggatcgt gtgcgttcga tggcggactg
     2581 gcggccgcga acggcggcgc gaccggcgcg gtcggtgtcg ggcgggtcgt ctccgatgca
     2641 cggttggtca ccctgcccgg gtgcccgatc aatgtcgaga atctcacggc cacgatcctc
     2701 cactacctga ccttccaggc attgcccgcg accgacgggc ggggccggcc gttgttcgcc
     2761 tacggcgcgc tcatccacaa ccagtgcggc cggcgatccc acttcgagtt cggcgagttc
     2821 gtccaggcct ggggcgatga aggcgctcag aagggctggt gcctctacaa gatgggctgc
     2881 aagggtcccg agaccttcgc caactgcccg tcggtccgct acggctccgg gacgagctgg
     2941 ccggtcaagg ccggcgccgc gtgcatcggc tgcacgacct ccggcttctg ggacgcgatg
     3001 agcccgttct cgcgacggct cccttcgccg atcccgttcg cgccggatgt cagcgtcgat
     3061 caggtcggcc aggcgctcgt cgggggcgtc ggcgtgctgg tcgcggccca cgggacggcg
     3121 agcattgccc gttcgaggtt gggacggaag cacggggtct tcggcgccgc aggcgaggcg
     3181 gaatcgttcg cgacggtcga agccgcgccg gcggctcagg tcgcgaccga gcctgagcca
     3241 tcgatgctcc tggccgcgga accgatcgat gcgtccgtgg cggagccggc tccaccggtc
     3301 gagccgccgg caccggagcc cgccgagccg gtcgacgccg gaccggtcga accggcggtt
     3361 accgacgtga ccgacgtgac cgaggcggcc gaggcggccg acgcgacgga cgcgccgggc
     3421 gcgaccgtcg acccgtcgcc gacggtcgac gccgctggcg cggccctcga cgatgcgtcg
     3481 gccgtcgacc caaccaggac ggacggccag tgagatgact cgcctcgtca tcgatcccgt
     3541 cagccgggtc ggcgggcacc tccgcgtcga ggtcgagctg accgaccgca ccgtcagcga
     3601 cgcgtggacc agcggcacga tgtaccgcgg gatcgaacgc gtcctcgagg gccgcgacgc
     3661 gcgcgatgcc tggctgtatg cccaacgcgt ctgcgggacg tgcgggatcg ctcacgccat
     3721 cgcgtccgct cgggcggtcg agaacgccct cgggctgaag atcccccgca acgcccgcct
     3781 gctccgcaac ctgatggccg gcgtccagct cgtcgtcgac cacgccgccg ggttctacct
     3841 gtcgcaagcg ctcgactggg tcgacctggc ctccgcgctc gatgcggacc cggcctcgac
     3901 gtcgaccctc gcccggacgc tgagcgactg gccgagctcc agcccgtcgt acttcacggc
     3961 ggtccagcaa cgcctggcga cgctcctcaa gtccgggcag ccgggcccgt tcgcccacgc
     4021 cgactggggt cacccggcgt acgccctcgc gcccgacgcc aacctgatgg tcgccgccca
     4081 ctacctcgag gcgctcgact ggcaccgcaa catgatgcgg atccaggtga tcctcggtgg
     4141 caagagcccc catccccaga cgttcctcgt cggcggcatg gcgctcgcgg cagcgtgggg
     4201 cggcccgatg cgaccgtccc ccggcgagca tcagtgggac ctcgagacga cgtcaccgcc
     4261 cgcgctcagc gccaacggcc tggccgacgt cggccgactc ctcgaccagg cgaaggcgtt
     4321 cgtcgagcag gtctacgtcc cggacgtcac cgcgatcctg aaggcctacg gcgggcagag
     4381 cccgaccgac gccggcatcg gccactatct ctccttcggc gacttccccg aagacgattc
     4441 ggaccgaccg gcgctcctcc tgccacgcgg tcgagtgatg ggcggcgatc tctccggcgc
     4501 cgtccccgtc agcgacaccg gggtcggcga gagcgtcgat cactcctggt acaccaccgg
     4561 cgccgacgac ggcgcgctcg tccatccgcg cgacgagcgg accgagcctc actacgccgg
     4621 cccccggccg ccgtacgcgg cactcgatgg cttcgatcgg tacagctggg tcaaggcgcc
     4681 ccggtacgag gacgacccga tggaggtcgg accgctggct cgcctcctcg tcgcggtcgc
     4741 gagcgcgggc gacgcggcga gcgccctctc gcgagcggtc gccgaggtcg gactggggcc
     4801 ggaggccctg ttcagcgggc tcggaagaac gggcgcccgg gcgatcgaat cgatgttcgt
     4861 cgcgaaacgg ctcagcgcgt ggctggcgga cctcaaggag aacatggcgg ccggcgacct
     4921 ggcgatggcc gacatctcga cctgggacgt tgaaacctgg ccggctgagg ccgcgggctg
     4981 ggcgatcggg gagtccgcgc gcggcgccgt cggccactgg ctgacgatcc ggaaccaccg
     5041 gatcgagcgc taccagatcg tcgacgcgac gacttggaac ggctcgccgc gcgaccggcg
     5101 cggccggcgt ggggcgatcg agcaggcgtt gatcgggacc cgggtcgtcg atcccgaccg
     5161 gccgctcgag atcctccgga ccgtccgctc cttcgacccc tgcctcacct gtgcggtgca
     5221 ctgatggacc tccaccgatg accgcgacgc cgctgctcca ggaacgcccc gccgggccgg
     5281 cggccatgga ggcagccccg gccgggcacg gagaggagcg cgtccggctc tatgtctggc
     5341 agcgacccgt ccggatcacc cactgggtga cggccggctg catcgcggtc ctggccctca
     5401 ccggcgggta catcgccgac ccgttcctca tcccgcccgg cggctccgtg atgacgacga
     5461 tgcgcttcat ccacatcgtc gccgcgttca ccctcctcgc gtctgggctc atccgcacgg
     5521 tgtggctgct cgtcggcaac cgcttcgccc gctggaacgc cttcatcccg acgacccgct
     5581 tccaggcgac ggaggtcttc cgccaggccg gcttctacgc gttcctgcgc aaggagatcc
     5641 ccaaggtcat cggccacaac cagctggccg ccgtcgccta cctcgtcctg ttcgtgctgc
     5701 tggtggtcga gacggtcacc gggatgaccc tcgacggcgc cgcgggcgcc gagccggcga
     5761 ggacgatgtt cggctggctt ggcgaggcgt tcggcctgca gtggatccga ctcgtccacc
     5821 acctcgcgat gtgggcgatc ctggccattg ccctgttcca cgtctacagc tgcgtcctga
     5881 tcgacaacat cgagaagaac ggcctgctct cgagcatggt cggcggcttc aagttcccga
     5941 cccgcgacga ggtcctcgag gctcgcgacg gcgggccgga gctgctcgag acgctcgaat
     6001 gagacggccc cgcctcccgt cccgcctctt cgtcgaacgc gcgatccggc tgcttgtcgt
     6061 cgccaatgtc gcggcgctcg gagtcttcgc cgtcgccgtg atcctcggcc actcgggcac
     6121 gccgggcagc cagccgccgc agatcgcccc acgcccgtcg gcgtcgttcg aattgatcgc
     6181 gatgggcaac gcccacatcc cgacgaccaa cgcgtgcatc ctctgccacg gcgccggcgg
     6241 ccaggtcaag accgtgccgg cgatcctcca cccgatcgag ggctggcggc gctgcaccac
     6301 ctgccaccag gacgagaacc tcgggagggc cgcgcccggc cacgacggca tcgccgagga
     6361 ggagtgcctg aactgccaca agaccgccca gaccggtccc gcgatcaccc agccgcatgc
     6421 cgcgctgcac gatcagcagt gcctcgcctg ccacggcagc gtcgcccacc tgccggccac
     6481 gatggcgagc tccagggagg ccgattgcgt gctctgccac cgggccgcga cgctgcctcc
     6541 gccgtcctac ccccatccac cggacgcccg cctgtcgtgt cggacctgcc accgatcggc
     6601 cgaggccggt gcgctcccga tcgaccacgc cctgcggacc gacacgacct gcctgctctg
     6661 ccacgacatc gcgatcgcgg cggcgggatc gccggctccg ggggcgacgc cggccgcgag
     6721 cctgctgctc gtgccgggcg ccagctcgtt gccgctgtcg agtccgagca ccgcgccgcc
     6781 gccgtaaccc gtaccctcgc aggatgatcc gcagcctgct cctctgggcc tcccgcaacc
     6841 gctggctgcg cgagcacctg tcgcggatgc ggttcgtccg ccgtgccgtc cgccggttca
     6901 tgccgggcga ggagctgacc gacgctctcg ccgccgccga gagctaccgc agcgacggca
     6961 tcggctccgt cttcaccctg ctcggcgaga acctgaccac gatcgccgac gccgaccggg
     7021 tggcggccca ttaccacgac gtgatcgacg agatcgcgaa gcgcggcctg gacggcgaga
     7081 tctcggtgaa gccgacccag ctcgggctgg acctcgaccc cgaggtgacg ttcgcccacg
     7141 tcgacgcgct cgcccgccac gccgcgaccg ccgggacctg ggtctgggtc gacatggagg
     7201 ggagcgccta cacggacgcg accgtcgccc tgtacgaacg ggccgcgagc gcccaccgaa
     7261 acgtcgggct gtgcgtccag gcgtacctcc ggcggacgcc cgacgacctc cgccggctgt
     7321 tcacggtcac cccgtcgatc cggctcgtca agggcgccta cgacgagccg gcgacgatcg
     7381 cctggcggag cgcccgcggg gtggacgccg cgttcgcctc ggtcgcgacc ctgctcctcg
     7441 aggcgcaggc gtccggtcgc gcggcgcggg tcgtcctggc cacccacgac acgaacctga
     7501 tcgcccagga gctgtcgatc ggcgccgcgc tcggtctcgc gcgggaccgg atcgaggtca
     7561 cgatgctcta cgggatccgg tccggcgagc agcgccgcct cgcgaaggag ggtgtcgcga
     7621 cccgggggct gatcgcctac ggcgactact ggtacccgtg gtacctgcgt cgcctcgccg
     7681 agcgccccgc caacgtcatc ttcgccctgc gggcgctcct gccctagcga ttcgatcggg
     7741 catgctcacg cggcggccgc gagcatgtcg gcgtacttct cgagggagcg ggcgaccttg
     7801 gccagcggct ccatcagggc gggcgtgcag gcctgaccgc agcggacggc gaacgcggcc
     7861 gcgtcgcgca gccgttcctc catcaggatc cacgcggccg cccgatgggg gagcagatag
     7921 tcgacctggt agaagagctc gaacgccggg cccgccgtga cgcccggcac atccatcccg
     7981 atgggtaggg tggtgacgac gctcccaagc ggcttgatgg ccgcgtacat caacccgacc
     8041 gacgcgtcgg ccaggacgcc gagctgctcc ggcgtctcgt ccgtgtgggc gaagtacctg
     8101 gacaggagct ggagcaggac ctcgtagacg acgttgagga ggtccatcga ccgggtcgtg
     8161 cccgggt
//
LOCUS       DATAIW010000413         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_191088, whole genome shotgun sequence.
ACCESSION   DATAIW010000413 DATAIW010000000
VERSION     DATAIW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191088"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VHM48_12325"
     CDS             complement(<1..671)
                     /locus_tag="VHM48_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2756246.1"
                     /translation="MSVDQRPEIENLLTEGRTFRPDPAFVAQANAQAELYEEAARDPE
                     AFWAKIARETVTWNEPFETTLEWDLPFAKWFVGGKLNMSENCVDRHVAAGLGDKVAYH
                     WIGEPGDTRTITYADLLREVSKAANALRELGIETGDRVAIYMPMIPELPIAMLACARI
                     GAPHTVVFGGFSAEALSGRINDAEAKLVITADGGWRRGKPAELKPAVDEAVQSTPTVE
                     HVLVVR"
     gene            complement(1125..2054)
                     /locus_tag="VHM48_12330"
     CDS             complement(1125..2054)
                     /locus_tag="VHM48_12330"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2756247.1"
                     /translation="MDFTKPPLSTIVDVLRTNGVPILVIFIVAIIALRVTTLLVHGIV
                     KTLLDREATEGTAQELSAIEVQKRMDTLDELMGRVLRAFIVMIAFIMVLGALGLDIGP
                     AVAGLGVVGIAVGFGAQSLVKDYLNGALILIENQYSKGDVVSIAGVSGAVEDFSLRRT
                     TLRDLDGIVHTVPNGEIKVASNRTRTWARINQNVTVAFGTDIDKAIEVVDELGKAMAA
                     DPIWKRRILEPPRVERVESIEERGITLKILGTVRASEQWAAGGEFRKLLLEAFAKNEI
                     RLPRPPQVFVAQSEATEGVAIGPAPTQDDLADE"
     gene            2134..3129
                     /locus_tag="VHM48_12335"
     CDS             2134..3129
                     /locus_tag="VHM48_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756248.1"
                     /translation="MFLVVSLAACGSSPSPSPSHDASPSVEASEPAAATPDPAAAEAA
                     IARVLDPGSRDLLALIAPEETIEATIDLADPKRAASAAMAQGLDGQVVSIGTVLVVGP
                     RDDVVGFAAGAQATRIAIAEDHRPDRWLPAEAPSIRIPAPGWPYRLGPMPVDRPGLLM
                     EDDQANVLLAELARKIETIDGRPYVRFTVTGECDPSKPPSCLLRASGVSVGVGDRSDG
                     YLVVSNKTTAGNPHLDSSTHESVPRDLRRAAEWIARHDPKALAEISSETGCCDARWDP
                     GRPGVVTVTWTRPCSAAVARPEAILADTGNCFDTLAIAVDLGRGTVVSIERAAGP"
BASE COUNT          464 a         1113 c         1113 g          496 t
ORIGIN      
        1 cggacgacga ggacgtgctc gaccgtcggc gtcgactgga cggcctcatc gaccgcgggc
       61 ttgagctcgg ccggcttgcc gcgccgccag ccgccgtcgg cggtgatgac gagcttcgcc
      121 tcggcgtcgt tgatccggcc cgacagggcc tccgccgaga acccgccgaa gacgacggtg
      181 tgcggcgcgc cgatccgggc gcaggcgagc attgcgatcg gcagctccgg gatcatcggc
      241 atgtagatcg cgacgcggtc gccggtctcg atcccgagct cgcggagggc gttggccgcc
      301 ttcgagacct cgcgcagcag gtcggcgtag gtgatcgtcc gcgtgtcgcc cggctcgccg
      361 atccagtgat aggcgacctt gtcgccaagg cccgcggcga cgtgccggtc gacgcagttc
      421 tcgctcatgt tgagcttgcc gccgacgaac catttcgcga acggcaggtc ccactcgagc
      481 gttgtctcga acggctcgtt ccaggtcacg gtctcgcgcg cgatcttcgc ccagaaggcc
      541 tcggggtcgc gggcggcctc ctcgtacagc tcggcctgcg cgttggcctg ggcgacgaac
      601 gccggatcgg gccggaaggt ccggccctcc gtgaggaggt tctcgatctc gggtcgctgg
      661 tcgacgctca cgtggccgct cctgccggtg gtcgggcgca cacatacaac acaggctgaa
      721 cggccattct acgcggcggg ctgatgagcg gcgctcggcg ggagtcagcg ggggctcgac
      781 atcaagcgcc ctgaggaccg cggacccgag gcggcggccg cggatccggc cagacattcg
      841 cccagagact gggcagccaa tcgactcgat ccccgccgac caacccgcaa gtcggccaga
      901 tactcgtcgg gagactcgtc gggcgcgatc gggctcgctc ggcatccctc ttgcgccgca
      961 gctggctcgc gtccacgcac gattcgcggg ttgtccgacc agatccgagc gaacgatatg
     1021 gccgactgtc cagcggagcg agcccaacga gtcctgtcag gaatatctgg ccgtcctggg
     1081 cggcggaatc ggcgatccgg gccacggatc ggcggcccgt gaccctactc gtcggcgagg
     1141 tcgtcctgag tcggtgccgg cccgatggca acgccctcgg tcgcctccga ctgggcgacg
     1201 aacacctgtg gtggccgcgg caggcggatc tcattcttcg cgaaggcctc gaggaggagc
     1261 ttgcggaact cgccgccggc cgcccactgc tccgacgcgc gcaccgtgcc gaggatcttg
     1321 agggtgatcc cccgttcctc gatcgactcg acccgctcga cccgcggcgg ctcgaggatc
     1381 cggcgcttcc agatcgggtc ggcggccatc gccttgccga gctcatcgac gacctcgatc
     1441 gccttgtcga tgtcggtccc gaaggcgacc gtgacgttct gattgatccg ggcccaggtg
     1501 cgcgtccggt tcgacgcgac cttgatctcg ccgttgggaa cggtgtggac gatcccgtcg
     1561 agatcgcgca gcgtcgtccg ccgcaggctg aagtcctcga ccgctccgct gacccccgcg
     1621 atcgagacga catcgccctt gctgtactgg ttctcgatga ggatcaacgc accgttgaga
     1681 tagtccttga cgaggctctg ggcgccgaac ccgacggcga tcccgacgac gccgagaccg
     1741 gcgacggccg gcccgatgtc gaggccgagg gcaccgagga ccatgatgaa ggcgatcatc
     1801 acgatgaatg cccggagcac ccggcccatc agctcgtcga gggtgtccat ccgcttctgg
     1861 acctcgatcg ccgagagctc ctgggccgtg ccctcggtcg cctcgcggtc gaggagcgtc
     1921 ttgacgatgc cgtggaccaa cagcgtcgtg acgcggaggg cgatgatggc cacgatgaag
     1981 atgacgagga tcggcacgcc gttcgtccgg aggacgtcga cgatcgtgct cagcggcggc
     2041 ttggtgaagt ccatcgcccg cgatggtagc ccggcgggca tctgccgatc cgggtcgagg
     2101 ctccggtccg tcgaccggcg ggggctggcg ggtttgttcc ttgtcgtctc gctggccgca
     2161 tgcggctctt ccccgtcgcc atcaccgtcc cacgacgcgt cgccgtcggt cgaggcctcc
     2221 gagcccgcgg cggcgacgcc cgatccggct gcggccgagg ccgccatcgc ccgtgtcctt
     2281 gatcccggca gtcgcgacct gctcgcgctc atcgcaccgg aggagacgat cgaggcaacg
     2341 atcgacctgg cagatcccaa gcgcgccgcg tccgccgcaa tggcccaggg cctcgatggt
     2401 caagtcgtgt cgatcgggac ggtgctggtc gtcgggccac gcgacgatgt cgtcggcttt
     2461 gccgcgggtg cgcaggcgac gcggatcgcg atcgccgagg accatcgccc ggatcggtgg
     2521 ctgccggccg aggcaccgtc gatccggatc ccggccccgg gctggccgta tcggctcgga
     2581 cccatgcccg ttgatcggcc ggggctcctc atggaggacg accaggcgaa cgtgctgctc
     2641 gccgaactcg cgcggaagat cgagacgatc gacggccggc cgtacgtgcg attcacggtc
     2701 accggcgagt gtgatccctc caagccgccg agctgcctgc ttcgcgcttc gggcgtgagc
     2761 gtcggtgtcg gcgatcggtc ggatgggtac ctggtcgtct cgaacaagac gaccgcgggc
     2821 aacccccacc tcgattcgag cacccacgaa tccgtgccgc gcgacctccg tcgggcggcc
     2881 gaatggatcg cacgccacga cccgaaggcc cttgccgaga tctcgagcga gaccggctgc
     2941 tgcgacgcca ggtgggatcc cgggcgcccg ggcgtggtaa ccgtgacctg gacgaggccc
     3001 tgctcagccg cggttgcccg gcccgaagcg attctggcgg acaccggaaa ctgcttcgac
     3061 accctcgcga tcgccgtcga cctcgggcgc ggcaccgtgg tctcgatcga gcgcgcggcc
     3121 ggaccctgag cggcggagga acgcggcagc cgcgcctagg acgcggcggc gacctcggcg
     3181 gcgagc
//
LOCUS       DATAIW010000414        12435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_117848, whole genome shotgun sequence.
ACCESSION   DATAIW010000414 DATAIW010000000
VERSION     DATAIW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117848"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VHM48_12340"
     CDS             <1..826
                     /locus_tag="VHM48_12340"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX2756249.1"
                     /translation="RLAAATLVASSFGHAAAIAPVEAAGGPILDELAATSRAAYRELV
                     EGSGFVAFFRLATPIDEIATLRFGSRPAARGRASAHAPAPAAAVRPPEPAAPDSIADL
                     RAIPWVFAWSQARIELPGWFGVGSALEAHAAAHGEAGIAALARLYARWPFFASLIDNA
                     ELALARADLGVARQYAALAGDTATGPWAAIEAEYARTVAWLARLTGRDRLLELEPELR
                     RRMALRDPYVDSLSEMQVTLLARLRAAAPDDPGRARLLRLVQLTVNGIAAGLQRTG"
     gene            complement(861..1451)
                     /locus_tag="VHM48_12345"
     CDS             complement(861..1451)
                     /locus_tag="VHM48_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756250.1"
                     /translation="MTVVLRRILVSAAVAAIVAVAAIGAARATAPTEPVHVVPGQIGV
                     APAPATVPVAPGAADPGDDALTAELDAVLAADQASPSAAPRAGERIAVRGQLRRLAAW
                     RRLVHATVVVDLKAGGLTTIQLDHGTISAMTSTTLTIAEAGGGSVSVALGDETRVRRD
                     GAKAAAADLRNGDEVFVMSKVEAGGASAYLVVVPKS"
     gene            complement(1588..1785)
                     /locus_tag="VHM48_12350"
     CDS             complement(1588..1785)
                     /locus_tag="VHM48_12350"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2756251.1"
                     /translation="MEGSMIAAAGLFMAGLVILYAVLVRAAGPRRRLETALGLEALSA
                     RRASGEITAEEFEQGRRLLGG"
     gene            complement(1848..2747)
                     /locus_tag="VHM48_12355"
     CDS             complement(1848..2747)
                     /locus_tag="VHM48_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX2756252.1"
                     /translation="MPARTDRMTRSRRPAAGDPRLGGTMSGPIGLHGGGEYLAGDEPF
                     LDALLEAASIAAAARAAASAGQPTPDGAADVRTFDVSGHAVTAAAEPIRVVILPTAAA
                     RGVPDRAAATGRDAFVRRAAAAGRRVVVEVARVVDAASADDPRTADRLAGADLIHLPG
                     GDPDLVPAILGDSRALAGLEAAWRRGAVIAGASAGAMALAEWTWTPGGGIRGLGLVAG
                     LAVVPHYDEIRRTRWQVTLDELAPGEIGYLGLDERTGVLSTANGSGATVWRVAGPGAA
                     YWFDRGASEPRTGRHGDLLGFAP"
     gene            2804..4144
                     /locus_tag="VHM48_12360"
     CDS             2804..4144
                     /locus_tag="VHM48_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756253.1"
                     /translation="MSLRPGDVVARIVAWTRRWDAILPLLAAELIVWLGFGALLPIMP
                     IYFTANGVDLRTLGIVVAAWPAARLVGEPIFGWVADRTRRVPLMIAGNVGAGIFLFLP
                     LVLVGAAPFVVLRALAGLATSIYDPAARGYITDATPADRRGEAFGMYGAAQMGGLLLG
                     PAIGGLGSAFFGGIGFIFVFGAVSSFVAAAAIAIRVRERPRAVDAERTGILDLTEFAH
                     EPDHLAARASRAPASAERALGDQPRAADEAPRSLANRLLVAAILFTVGGNFAAGTYEV
                     IWSLYLQSLGAGLELIGLTFAMFGLPVLVLSTYFGRRVDRGSLAPYVLIGTIIPVTAA
                     LLYTVIRDPLLSIPLILAEATGFAMLNPALFSIVAAGSPIGRPSTAQGLFGAAGTLGF
                     VVASLLTGALAEIDIRLPFYVFAVVMTTFTTIGFLVGGVRLRRRSAVRVEGRAA"
     gene            complement(4217..4414)
                     /locus_tag="VHM48_12365"
     CDS             complement(4217..4414)
                     /locus_tag="VHM48_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756254.1"
                     /translation="MFRRHRTDPDRELATVVRAFHDRLAGSLVTTRTDPPRVDPRNLR
                     GRLGTPAVGLRDRIGRPLGTV"
     gene            4778..4852
                     /locus_tag="VHM48_12370"
     tRNA            4778..4852
                     /locus_tag="VHM48_12370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4810..4812,aa:Met,seq:cat)
     gene            complement(4981..5748)
                     /locus_tag="VHM48_12375"
     CDS             complement(4981..5748)
                     /locus_tag="VHM48_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756255.1"
                     /translation="MSRPTSPVRAVAALTAMELRLVLRRGENLFATIVIPTLVLVLFS
                     SISILPTGTGRAVDFLLPGAIALGIVATSLVSLGITTAYDRSYGVLKRLGGSPLSRAE
                     LVTARLLTVLVVELVQIALLVTTATVVLGWTAGAGGAGSPLLFAASVLLGTIAFAGLG
                     LLLAGTLRAETVLAVANVLFLGLLVVGGIIVPIDRLPGPLAAIAAALPAAPLAELLRS
                     ALGSVAVAGADLGSAMALLGAWAVAAVGLAAATFRWE"
     gene            complement(5745..6782)
                     /locus_tag="VHM48_12380"
     CDS             complement(5745..6782)
                     /locus_tag="VHM48_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756256.1"
                     /translation="MSAAVADRTLPAIEVSGLRKSYDHRPVLRDLSFRVAAGEIFALL
                     GPNGAGKTTTVEIIEGYRRADRGEVRVLGVDPARAGRAHRARVGLMLQGGGGIDPRMT
                     AREVVGLHARFHAEPRDVDELLGRVGLTGSTARTRYRRLSGGEKQRVGLALALVGRPE
                     LAILDEPTAGMDVEARAATRDLLAGLRASGVTVLLTSHDLVDVERLVDRLAILDRGRI
                     VAIGAPQELTAASFPVLRFRLALPLAEPDRVALADRLAEAGGDSRIAIEDDGGSGRYR
                     VDGLAPTPPIVATVASWCEARGALILELRTGGGSLEERYLELIGAAGDEPIDDDAADD
                     VRGGRRRRRGR"
     gene            complement(6829..7410)
                     /locus_tag="VHM48_12385"
     CDS             complement(6829..7410)
                     /locus_tag="VHM48_12385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2756257.1"
                     /translation="MTEPQTPTSEPFLVVARRPRPILPITLLLLVAIATTAIVVLVAR
                     GGPTDTSGIGKGKAVPNVAGTTLTGKTFDLASLRGKPVLINFWGPGCVPCREEFPLLA
                     AKAVEHQASGLVIVGVLTDDPVEPARAFAAQYGGTWETVIDPGAAIKAAYHVIARPQT
                     YFVDRNGVLQSIQPGQLTDADFERQFAQIAGGG"
     gene            complement(7420..8508)
                     /locus_tag="VHM48_12390"
     CDS             complement(7420..8508)
                     /locus_tag="VHM48_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2756258.1"
                     /translation="MQLLENIWNGILDLTKLLVIPDWGALIALLPVFMGVLVIVFFLG
                     RVLQYRRLGPRRRRPGRVKPVTPPGLHMPGPTYAPIFAAIGTFLLFMGLVFNGLILVL
                     GLVALLLTLLYWGREGLTDYDHVAGDHPQLPAVVHDGPPPGVHMPGPSFRPILASLGV
                     AVLFAGLVFGGWVLAVGVIFTIAALLGWLSDARKEYRQVERADTTGHLENEPAPGWPR
                     ALLSVFAVLVIAAIALNAGWLPPRSASGESGGPSGSAAPSGGPAGSGAPGAGGLAISA
                     SGVKFDVATLTAPADKPFTIHFDNKDIAGTPHDVDIFDASGAKVVDNKVISAPSVQDY
                     PIPPLKAGTYKFACSIHPTMTGQLTVGG"
     gene            complement(8543..8737)
                     /locus_tag="VHM48_12395"
     CDS             complement(8543..8737)
                     /locus_tag="VHM48_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756259.1"
                     /translation="MHPLRHPRNAALGGIIFIIIAAIFWAVPYYGGWHIDYAGVTMLA
                     VLGIAMALMAYVLIAGSPND"
     gene            complement(8751..9443)
                     /locus_tag="VHM48_12400"
     CDS             complement(8751..9443)
                     /locus_tag="VHM48_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX2756260.1"
                     /translation="MATNASALTTGDPGHAVDEHNPMAGMAHDARGGISNPILGMMLF
                     ICSEVMFFSGLFAAYFSVRATSVHWPPIVAGGKDPASQAAALALTEHFNLHAEPFYAL
                     GLTVILIISSVTCQLGVWAIRRNDRTGFIRAIGVTLVLGIVFLLGQLYDYSTIGFGVN
                     DTPFGTTFYTLTGFHGAHVFGGAVMLSVVLYRGLAGQFSSKHHDAVEAVSLYWHFVDV
                     VWIALFSTLYIL"
     gene            complement(9443..11074)
                     /gene="ctaD"
                     /locus_tag="VHM48_12405"
     CDS             complement(9443..11074)
                     /gene="ctaD"
                     /locus_tag="VHM48_12405"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX2756261.1"
                     /translation="MATTTLTPAASTYRSGLYEWLTTTDHKRIGILYLVNSFLFFFIG
                     GMLALGVRTELAQPGLQLVHDESIYNQLFTMHGTVMIFLFIIPMLAGFGNYVVPLQIG
                     APDMAFPRINALSFWMLPLAGILLLMGFVTGGAASAGWTSYSPLAQDRPLGSTGSGED
                     LWIMALVLIGTSSILGSINFLVTIFKMRAPGMTMFRMPILVWTVLVTSVLVLMATPVI
                     TSALIMLFIDRNYGGHFFDPVAGGNAILWQNVFWFYSHPAVYVMVLPAMGMISEILPV
                     FSRKPLFGYKAFVFATAGIGALGFSVWAHHMFTTGSVYLPFFSLMTFFIAVPTGVKMF
                     NWIFTLFRGKLTFSTPLLFALGFLSMFLIGGINGAFSAAVPVDFALHDTYWVVAHLHY
                     VLFGGSVFGVMGGLYYWFPKMTGRMLGEGLGKVQFLLMFTGFNLTFFPMHQLGLAGMP
                     RRIADYASDAGWNDLNLAATIGGFMIAASMIPLLWNVFISLRAGRIAGDDPWEANTLE
                     WATTSPPPPYNFDHLPEIRSERPLFDLRHGRTASH"
     gene            complement(11085..12221)
                     /gene="coxB"
                     /locus_tag="VHM48_12410"
     CDS             complement(11085..12221)
                     /gene="coxB"
                     /locus_tag="VHM48_12410"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2756262.1"
                     /translation="MSSTPGSRPSTSAIFGWLIGAAVLIALAALVVLNAQSIWTSFYP
                     PVAKSVQGEQIRSLYDIVFGIAVAIFVLVEGLIIWTVLRYRRKPGDDVLPPQTHGNNI
                     AEITWVLIPTLIVLYLFVISWQTLNSVEAVSAHPNLTVRANAGQFQWSFDYLPADATD
                     TTKAVFSVTTPTGPDGGLTVPAGQTIHLFLHSPDVIHAFYVPQFLFKRDVVPHVEGQP
                     DNAFDLTIPASDAGQTYRGQCAELCGIGHRIMLFEVHVLAPDAFQAWYDKQLAAANAT
                     PPPAASGAAAGPTIPLTAQGVKFDQSTLTGPATGFTIHFDNKDTSGTPHDVDILDASG
                     AKVVDNKVITAPAVQDYPIPPLPAGTYKFQCSIHPTMTGTLTVQ"
BASE COUNT         1850 a         4313 c         4547 g         1725 t
ORIGIN      
        1 gcgcctcgcg gcggcgaccc tcgtcgcgtc gtcgtttggc catgccgcgg cgatcgcacc
       61 ggtcgaagcc gcgggcgggc cgatcctcga cgagctggcc gcgacgtccc gggcggcgta
      121 ccgcgagctg gtcgaggggt ccgggttcgt ggcgttcttc cggctggcga ccccgatcga
      181 cgagatcgcg acgctacgct tcggatcgcg gccggccgcc cgcggccggg cgtcggcgca
      241 tgcgcccgcg cccgcagcgg ctgtccggcc gcccgaaccc gccgcgcccg actcgatcgc
      301 ggacctgcgc gcgatcccgt gggtcttcgc ctggtcgcag gcccggatcg agctgccggg
      361 ctggttcggc gtcggttccg cgctcgaggc gcacgccgcc gcccacgggg aggccgggat
      421 cgcggccctc gcccggctct acgcccgctg gccgttcttc gccagcctga tcgacaacgc
      481 ggagctcgcc ctcgcccggg ccgacctcgg cgtcgcccgc cagtacgcgg cgctcgccgg
      541 cgacacggcg accggcccgt gggccgcgat cgaggccgaa tacgcccgga ccgtcgcctg
      601 gctcgcccgc ctgacgggac gcgaccggct cctcgagctc gagcccgagc ttcgccggcg
      661 gatggccctc cgcgacccgt acgtcgactc actctcggag atgcaggtca cgctgctcgc
      721 ccggctccgg gccgccgccc ccgacgatcc gggccgggcc cggctcctcc gcctcgtcca
      781 gctcaccgtg aacgggatcg ccgcggggct ccagcggacg ggctgacccg atccctcgcg
      841 atcttcgacg gggatcgggg tcagctcttc gggacgacga ccaggtaggc gctcgccccg
      901 ccggcttcga ccttgctcat gacgaagacc tcgtcgccgt tcctgagatc ggcggcggcg
      961 gccttcgcgc cgtcgcgccg gacgcgcgtc tcgtcaccga gggcgacgga caccgatccg
     1021 ccgccggcct ccgcgatcgt gagcgtcgtc gaggtcatcg ccgagatcgt tccgtggtcg
     1081 agctggatcg tggtcagccc gcctgccttc aggtcgacga cgacggtcgc gtggaccagg
     1141 cggcgccacg cggcgagacg ccggagctgg ccgcggaccg cgatgcgctc gccggcccga
     1201 ggagccgcgg agggggaggc ttgatccgcc gcgaggacgg cgtcgagctc ggcggtcagg
     1261 gcgtcgtcgc cgggatcggc cgcgccgggg gcgacgggca ccgtggccgg tgctggtgcg
     1321 acgccgatct ggccgggcac gacgtggacc ggctcggtcg gagcggtcgc gcgtgcggcg
     1381 ccgatggccg cgacggcgac gatggcggcg acggccgcgc tgacgaggat gcgacgcagg
     1441 acgacggtca tcgatcgggg ctccgtgctg gtgatcggcg gccgattgcc ggtcgcgctc
     1501 cgccgacgat cgcgtcccgt gatgaggcga tccagcccgc tacgtaaagg ccatgtaagg
     1561 cggtcgggcg ggtcagcccg tcgcgcgtca gccgccgagg agccgccggc cctgctcgaa
     1621 ctcctcggcc gtgatctcgc cgcttgcgcg gcgggcactg agcgcctcga gacccagtgc
     1681 ggtctcgagc cggcgtcgtg gaccggccgc gcgcaccagc acggcgtaca ggatgacgag
     1741 cccggccatg aagaggccgg ccgcggcaat catcgagcct tccatgggcc gatactaccg
     1801 cgtgccgggc ctgcgcggaa gggcggcgaa cgggcggaag gacgggctca cggggcgaag
     1861 ccgaggaggt cgccgtggcg tccggtccgc ggctccgacg cgccacgatc gaaccagtac
     1921 gcggccccgg gcccggcgac tcgccagacc gtcgctccgg agccgttcgc cgtggagagg
     1981 acccccgtac gctcgtcgag cccgaggtag ccgatctcgc ccggtgccag ctcgtcgagg
     2041 gtgacctgcc accgggtccg gcgaatctcg tcgtagtgcg ggacgaccgc gagcccggcg
     2101 acgaggccca ggccgcggat cccgccgccc ggcgtccacg tccactcggc gagggccatc
     2161 gcgcccgcgc tcgcacccgc gatgaccgcg ccgcgtcgcc acgcggcctc gaggccggcc
     2221 agggcgcggc tgtcgccgag gatcgcaggc acgaggtcgg ggtcgccgcc cggcaggtgg
     2281 atgaggtcgg cgccggcgag ccgatcggca gtccggggat cgtccgcgct cgccgcgtcg
     2341 acgacccgtg ccacctcgac gaccacgcgc cggcccgcgg cggctgcgcg ccgcacgaag
     2401 gcgtcccggc ccgtcgcggc cgcacgatcc ggcacgcccc gggcggcggc cgtcggcagg
     2461 atcaccaccc ggatcggctc ggcggccgcc gtgaccgcgt ggccgctcac gtcgaaggtg
     2521 cgcacgtcgg ccgcgccgtc cggggtcggc tgcccggcgc tcgctgccgc gcgggccgcc
     2581 gccgcgatcg aggcggcctc gagcagcgcg tcgagaaagg gctcatcgcc ggcgagatac
     2641 tcgccgccgc catgcaggcc gatcggtccg ctcatcgttc ccccaagacg aggatcgccg
     2701 gctgcgggtc gccggcttcg ggtcatccgg tcggtcctcg caggcaccgc tcgatgatac
     2761 gacccggccg tttgccgtcc gtcgcgccta gcatcagccg cccatgtccc tccgccctgg
     2821 cgatgtcgtc gcccggatcg tcgcctggac ccgccgctgg gacgcgatcc tcccgctcct
     2881 cgccgccgag ctgatcgtct ggctcggctt cggcgcgctg ctcccgatca tgccgatcta
     2941 ctttacggcc aacggggtcg acctccgcac cctcggcatc gtcgtggccg cctggccggc
     3001 tgcccggctg gtcggcgagc cgatcttcgg ctgggtggcg gatcggacgc ggcgggtccc
     3061 gctgatgatt gccggcaacg tcggggccgg gatcttcctg ttcctgccgc tcgtcctggt
     3121 cggggcggcg ccgttcgtcg tcctgcgcgc cctcgccggg ctcgccacct cgatctacga
     3181 ccctgccgcg cgcggctaca tcaccgacgc gacgccggcc gatcgccgcg gcgaggcgtt
     3241 cgggatgtac ggggcggccc agatgggcgg cctcctgctc gggccggcga tcggtggcct
     3301 cgggtcggcg ttcttcggcg ggatcggctt catcttcgtg ttcggcgcgg tgagctcatt
     3361 cgtcgccgcc gcggcgatcg cgatccgcgt ccgggagcga ccgcgggccg tcgatgccga
     3421 gcggaccggg atcctcgatc tcaccgagtt cgcccacgag ccggatcatc tcgccgcgcg
     3481 cgccagccgg gcgccggcga gcgcggagcg cgcgctcggg gaccagccgc gggcggccga
     3541 cgaggcgccg cggtccctgg cgaaccggct cctcgtggcg gcgatcctgt tcacggtcgg
     3601 cggcaacttc gcggccggca catacgaggt gatctggtcc ctgtacctcc agagcctcgg
     3661 cgccggcctc gagctgatcg ggctgacctt cgcgatgttc ggcctgccgg tgctggtgct
     3721 ctcgacgtat ttcgggcggc gggtcgatcg cggcagcctg gcgccgtatg tcctgatcgg
     3781 gacgatcatc ccggtcacgg ccgccctcct ctacaccgtg atccgcgacc cactgctgtc
     3841 gatcccgctg attctcgccg aggcgaccgg cttcgcgatg ctcaacccgg ccctgttctc
     3901 catcgtcgcc gccggcagcc cgatcggtcg gccgtccacg gcccagggcc tgttcggcgc
     3961 ggcgggcacg ctcgggttcg tggtcgcatc gttgctgacg ggggcgctcg ccgagatcga
     4021 catccgcctg ccgttctacg tgttcgcggt cgtcatgacc acgttcacga cgatcggctt
     4081 cctcgtcggc ggtgtccggc ttcgacgccg ttcggcggtc cgggtcgagg gtcgggcggc
     4141 ctgaccgcca cccggccccg accgggaacg ccgaacgatg ggtcgccggt cgagcgaccc
     4201 gatccggttc gaagcctcaa acggtgccga gcgggcggcc gatccgatcg cgcagcccga
     4261 cggccggcgt cccgagtcgt ccacgaaggt tccgcgggtc gacccgtggc gggtcggtgc
     4321 gcgtcgtcac cagcgagccg gcgagccggt cgtggaaggc gcggacgacc gtcgcgagct
     4381 cgcggtccgg atcggtgcgg tgacgtctga acatgtcggg gacagtgcgc cgcccggtgc
     4441 gccgaggcaa ccccgtcatt ctgaccatgc ccggtgggac ttccgtacct gcggacgggg
     4501 gcgcgacccg cggactcggc cgcgtcccgt cggacttgtc caatgccgcc gtgtggcgca
     4561 ttgccggcgt agcggctgcc tccgcggctc tcgaacttct gcaatgcggt cctggtcggc
     4621 agtggaacgg gaatcggcgt gatctggccg gttcgggcga ggtctgcgtc ggcccgcgcg
     4681 ccgtagcgcg gccatccgcg gcattgccca ggtcggactg cgacgaccgg cgtctctgcg
     4741 tttgacccgg acgcgattgg tcgctaccat gggcagtcgc ggggtggagc agcggcagct
     4801 cgtcgggctc ataacccgaa ggtcggcggt tcgaatccgt cccccgcaac caagccgcat
     4861 gcacctgaac cccgggtccg accgcccggg gttcttcttt gcccgggatc cggacctgga
     4921 tcggctcgag tgggcgcgta acaggggcgt ccaggacccg cggcccaccc gacggttggc
     4981 ctactcccag cggaacgtcg cggcggccag gccgaccgcc gcgaccgccc acgcgccgag
     5041 cagggccata gcgctgccca ggtcggcgcc ggcaacggcc accgacccca gcgccgagcg
     5101 cagcagctcg gcgagcggcg ccgccggcag cgccgcggcg atcgcggcca gcggtccggg
     5161 cagccggtcg atcgggacga tgatcccgcc gaccacgagg agcccgagga acaggacgtt
     5221 ggcgaccgca aggaccgtct cggctcgcaa cgtgccagcg agcagcaatc cgagccccgc
     5281 gaacgcgatc gtgccgagca ggacggacgc tgcgaacagc agcggcgagc cggccccgcc
     5341 ggcgcccgcc gtccagccga gcacgacggt cgcggtcgtg accagcagcg cgatctggac
     5401 gagctcgacg acgaggacgg tgagcagccg tgccgtgacg agctcggccc ggctgagcgg
     5461 cgagccgccg agccgcttga ggacgccgta cgagcggtcg tacgcggtcg tgatcccgag
     5521 gctgaccagg ctcgtcgcca cgatcccgag ggcgatcgcg cccggcagga ggaagtcgac
     5581 cgcgcgcccg gtgccggtcg gcaggatcga gatcgacgag aacaggacga ggacgagcgt
     5641 cgggatgacg atcgtcgcga acaggttctc gccgcggcgg aggacgagcc ggagctccat
     5701 cgcggtcagc gcggccaccg cgcggacggg cgacgtcggg cggctcatcg cccgcgccgc
     5761 cgtcgacgac cgccccggac gtcatccgcg gcgtcgtcgt cgatcggttc atcgccggcc
     5821 gcgccgatca gctcgagata ccgctcctcg agcgacccgc cgccggtccg cagttccagg
     5881 atcagcgcgc cccgcgcctc gcaccacgac gccacggtcg cgacgatcgg cggagtcggc
     5941 gccaggccgt cgacccgata gcggcccgac ccgccgtcgt cctcgatcgc gatccggctg
     6001 tcgccaccgg cctcggccag tcgatcagcc agcgcgaccc gatcgggctc ggccagcggc
     6061 agcgcgaggc ggaagcgtag gaccgggaac gaggccgcgg tcagctcctg cggcgcgccg
     6121 atggcgacga tccggccgcg gtcgaggatc gcgaggcggt cgaccagccg ttcgacatcg
     6181 acgaggtcgt ggctcgtcag gagcacggtg acgccgctcg cccggagccc ggcgagcagg
     6241 tcgcgcgtgg cggctcgcgc ctcgacgtcc atgccggcgg tcggctcatc gaggatcgcg
     6301 agctccggcc ggccgacgag ggccagcgcc agcccgaccc gctgcttctc gccacccgac
     6361 aggcgccggt agcgcgtccg cgcggtcgaa cccgtcagcc cgacacggcc gaggagctcg
     6421 tcgacgtcgc gcggctcggc gtggaaccgg gcatggaggc cgacgacctc gcgcgcggtc
     6481 atccgcgggt cgatcccgcc accgccctgg agcatcagcc cgacccgtgc ccggtgggcc
     6541 cgcccggctc gggccgggtc gacgccgagg acccggacct cgccgcgatc ggcccggcga
     6601 tagccctcga tgatctcgac cgtcgtcgtc ttgccggcgc cgttcggccc gagcagggcg
     6661 aagatctcgc ccgcggcgac ccggaaggac aggtcgcgca ggaccggccg atggtcgtag
     6721 gacttgcgga gcccggaaac ctcgatcgcg gggagcgttc gatcggcgac cgcggcgctc
     6781 atcgccgacc ggtgcccgcc gggccggtgc ccgcggctcg ggcgccgttc aacccccgcc
     6841 cgcgatctgc gcgaactggc gctcgaagtc ggcgtcggtg agctgtcccg gctggatcga
     6901 ctggagcacc ccgttccggt cgacgaaata ggtctgcggc cgggcgatca cgtgatacgc
     6961 cgccttgatc gccgcgcccg ggtcgatgac ggtctcccag gtcccgccgt attgggcggc
     7021 aaaggcccgg gcgggctcga ccggatcgtc ggtcaggacg ccgacgatca cgagcccgct
     7081 cgcctggtgc tcgaccgcct tcgcggcgag gagcgggaat tcctcgcggc acgggacgca
     7141 gccgggtccc cagaagttga tcaggaccgg cttgccgcgg aggctggcga ggtcgaaggt
     7201 cttgccggtc agcgtcgtcc ccgcgacgtt cgggacggcc ttgcccttcc cgatccccga
     7261 cgtgtcggtc ggtccgccac gggccacgag cacgacgatc gccgttgtcg cgatcgcaac
     7321 gaggaggagc agcgtgatcg gaaggatcgg gcgcggccgg cgggcgacca cgagaaacgg
     7381 ctcggacgtc ggggtctgtg gttcggtcac gtgcccggct cagccgccga cggtcagctg
     7441 cccagtcatc gtcgggtgga tcgagcaggc gaacttgtag gtcccggcct tgagcggcgg
     7501 gatcggatag tcctggactg acggggcgct gatgaccttg ttgtcgacga ccttggcgcc
     7561 gctggcatcg aagatgtcga cgtcgtgcgg cgtcccggcg atgtccttgt tgtcgaaatg
     7621 gatcgtgaac ggcttgtcgg ccggggccgt gagggtcgcg acgtcgaact tgacgccaga
     7681 cgccgagatc gcgagcccgc cggcgcccgg ggcacccgag cccgccggac cgcccgacgg
     7741 ggccgccgag cccgacggcc caccggattc acccgatgcc gatcgcggcg gcagccagcc
     7801 ggcgttgagg gcgatcgccg cgatgacgag gacggcgaag accgacagca gcgccctcgg
     7861 ccagccaggc gccggctcgt tctcgaggtg acccgttgtg tccgcccgct cgacctggcg
     7921 gtactccttg cgcgcgtcgc tcagccagcc gagcagcgcc gcgatcgtga agatgacccc
     7981 gactgccagg acccagccgc cgaacacgag accggcgaac aggaccgcga cgccgaggga
     8041 ggcgaggatc ggccggaagg acggtcccgg catgtggacg ccgggtggcg ggccgtcgtg
     8101 gacgacggcc ggcagctgcg ggtgatcgcc cgcgacgtgg tcgtagtcgg tcaggccctc
     8161 gcggccccag tagagcaggg tgagcaggag cgccacgagg ccgagcacca ggatcagccc
     8221 gttgaagacg aggcccatga acagcaggaa ggtgccgatc gcggcgaaga tcggggcgta
     8281 ggtcgggccc ggcatgtgga ggcccggggg cgtcacgggc ttgaccctgc ccggccgtcg
     8341 acgacgcggc ccgagccggc ggtactgaag gacccgaccg aggaagaaga cgatgacgag
     8401 gacgcccatg aagaccggca ggagcgcgat gagggcgccc cagtccggga tgacgaggag
     8461 cttcgtcagg tcgaggatcc cgttccagat gttctcgagg agctgcatcg gtcagtaatt
     8521 cagtcggccg tcgattgccg cgtcagtcgt tcggtgagcc ggcgatcagg acgtaggcca
     8581 tcagggccat cgcgatcccg agcacggcga gcatcgtcac gccggcatag tcgatgtgcc
     8641 agccgccgta gtagggcacg gcccagaaga tcgcggcgat gatgatgaag atgatcccgc
     8701 cgagggcggc gttgcgaggg tgtcggagcg ggtgcatcga gcctccctgc ctacaggata
     8761 tacagcgtcg aaaacagggc gatccagacc acgtcgacga agtgccagta gagggacacc
     8821 gcctcgaccg catcgtggtg cttcgagctg aactggccgg caaggccacg atagagcacg
     8881 accgacagca tcaccgctcc gccgaagacg tgcgcgccgt ggaagccggt cagcgtgtag
     8941 aacgtcgtcc cgaacggcgt gtcgttgacc ccgaagccga tcgtcgagta gtcgtacagc
     9001 tggccgagca ggaagacgat cccgagcacg agggtcaccc cgatcgcccg gatgaacccg
     9061 gtccggtcgt tgcgccggat cgcccagacg ccgagctgac aggtgaccga gctgatgatc
     9121 aggatgacgg tcaggccaag cgcgtagaac ggttcggcgt ggaggttgaa atgctcggtc
     9181 agggccaacg ccgccgcctg cgacgccggg tccttgccgc ctgcgacgat cggcggccag
     9241 tggaccgagg tcgcccgcac gctgaagtag gccgcgaaca ggccggagaa gaacatcacc
     9301 tccgagcaga tgaacagcat catcccgagg atcgggttgc tgatcccgcc gcgggcgtcg
     9361 tgggccatgc ccgccatcgg gttgtgctcg tcgacggcgt gacccggatc gccggtcgtc
     9421 agagcgctcg cgttcgtcgc catcagtggc tcgccgtccg gccgtggcga aggtcgaaga
     9481 gcgggcgctc cgagcggatc tcgggcaggt ggtcgaagtt gtacggcggt ggcggtgagg
     9541 tcgtggccca ctcgagggtg ttggcctccc acggatcgtc accggcgatc ctgcccgcac
     9601 ggagggagat gaagacgttc cagaggagcg ggatcatgct cgccgcgatc atgaacccgc
     9661 cgatcgtcgc cgccaggttc aggtcgttcc agccggcgtc cgacgcgtag tcggcgatcc
     9721 gtcgcggcat gccggccagg ccgagctggt gcatcgggaa gaacgtcagg ttgaagccgg
     9781 tgaacatcag caggaactgg accttgccga gcccctcgcc gagcatccgc ccggtcatct
     9841 tcgggaacca gtagtagagc ccgcccatca ccccgaacac cgacccgccg aacaggacgt
     9901 aatggaggtg ggcgacgacc cagtaggtgt cgtggagggc gaagtcgacc ggcaccgccg
     9961 ccgagaacgc gccgttgatc ccgccgatca ggaacatcga caggaacccg agggcgaaca
    10021 gcagcggggt cgagaaggtc agcttgccgc ggaacagggt gaagatccag ttgaacatct
    10081 tcaccccggt cgggaccgcg atgaagaacg tcatcaggct gaagaacggc aggtagaccg
    10141 agccggtcgt gaacatgtgg tgggcccaga ccgagaagcc gagggcgccg atcccggccg
    10201 tcgcgaagac gaacgccttg taaccgaaga gcggcttgcg cgagaagacc ggcaggatct
    10261 cgctgatcat gcccatcgcc ggcaggacca tgacgtagac ggccgggtgg gagtagaacc
    10321 agaacacgtt ctgccagagg atcgcgttgc cgcccgcgac cgggtcgaag aagtgaccgc
    10381 cgtagtttcg gtcgatgaag agcatgatca gggcgctggt gatgaccggc gtggccatca
    10441 gcaccaggac gcttgtcacg aggaccgtcc acacgaggat cggcatccgg aacatcgtca
    10501 tcccgggcgc ccgcatcttg aagatcgtga cgaggaagtt gatcgagccg agaatcgagc
    10561 tcgtcccgat caggacgagg gccatgatcc acaggtcctc accggagccc gtcgacccga
    10621 gcggtctgtc ctgggcaagc gggctgtagc tcgtccagcc cgccgatgcc gcgccgccgg
    10681 tcacgaagcc catcagcagc aggatcccgg ccaggggcag catccagaac gacagggcgt
    10741 tgatccgcgg gaacgccatg tcgggcgcac cgatctggag cgggaccacg tagttcccga
    10801 accccgccag catcggaatg atgaacagga agatcatcac cgtcccgtgc atcgtgaaga
    10861 gctggttgta gatgctctcg tcgtggacga gctggaggcc cggctgggcg agctcggtcc
    10921 ggacgccgag cgccagcatt ccgccgatga agaagaacag gaacgagttg acgagataga
    10981 ggatcccgat ccgcttgtgg tcggtcgtcg tcagccattc gtagaggccg ctccggtagg
    11041 tggaggcggc gggcgtcagc gtcgtggtcg ccatcggagc tcccttactg gaccgtgagc
    11101 gtgccggtca tcgtgggatg gatcgagcac tggaacttgt aggttccggc cgggagcggc
    11161 gggatcggat agtcctgcac ggccggcgcg gtgatgacct tgttgtcgac gaccttggcg
    11221 ccgctcgcgt cgaggatgtc gacgtcgtgc ggcgtcccgg aggtgtcctt gttgtcgaag
    11281 tggatcgtga acccggtcgc cggcccggtc agggtcgact ggtcgaactt cacgccctgg
    11341 gcggtcaacg ggatcgtcgg tccggcggcc gcgccgctcg cagccggcgg cggcgtcgcg
    11401 ttggccgcgg cgagctgctt gtcgtaccag gcctggaagg cgtccggggc caggacgtgg
    11461 acctcgaaca gcatgatccg gtggccgatt ccgcagagct cggcgcactg gccacgatag
    11521 gtctggccgg cgtcgctggc ggggatcgtc aggtcgaacg cgttgtcggg ctggccttcg
    11581 acatgcggca cgacgtcgcg cttgaagagg aactgcggga cgtagaaggc gtggatgacg
    11641 tccgggctgt gaaggaacaa gtggatcgtc tgaccggccg gcacggtcag gccgccatcg
    11701 ggcccggtcg gggtcgtgac cgagaacacc gccttggtcg tgtcggtcgc gtcggccggc
    11761 aggtagtcga aggaccactg gaactggccg gcgttcgccc ggacggtgag gttcggatgg
    11821 gcgctgacgg cctcgacgct gttgagcgtc tgccaggaga tgacgaagag gtagaggacg
    11881 atcagggtcg ggatcaggac ccacgtgatc tcggcgatgt tgttgccatg ggtctggggc
    11941 ggaagcacgt cgtcaccggg ctttcgccga tagcgcagga ccgtccagat gatcagcccc
    12001 tcgaccagga cgaagatcgc gacggcaatg ccgaagacga tgtcgtagag gctccggatc
    12061 tgctcgccct ggacgctctt cgcgacgggc ggatagaagc tggtccagat cgactgggcg
    12121 ttgaggacca ccagggccgc cagcgcgatc aggacggcag cgccaatcag ccagccgaag
    12181 atcgcgctcg ttgatggcct gctgcccggg gtggacgaca tcgatggtcc tcgaactagg
    12241 tggcgacggg atgccgaagc gggacgccgc caccaggtga agatcggagc cggccagaag
    12301 gccgcatccg acacgcgtcg gatcggcgcc catcataacg gaggagcctc gtcgagcgtg
    12361 gcgtcacatc gccgcccgcg aggaccgcca catcgccgca catcgccgcc ggtggggcga
    12421 ccgtgagacg acgcg
//
LOCUS       DATAIW010000415         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_177764, whole genome shotgun sequence.
ACCESSION   DATAIW010000415 DATAIW010000000
VERSION     DATAIW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177764"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VHM48_12415"
     CDS             complement(<1..816)
                     /locus_tag="VHM48_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2756263.1"
                     /translation="MRIVSLLPSATEIVCALGLRDELVGVTHECDWPPEVVGLPVMTS
                     NALDLVGASSREIHRRVGDAVHGGSAIYHLDEDALEVADADLILTQELCAVCAVGYRQ
                     VSEAVRALEMDATVVSLEPTSVEGILNTITTVGAMAEAEDAAIELVESLRSRLGAIES
                     KAQERRERGFVGPRVVGLEWLDPPFAVGHWVPDQIRRAGGWDVLGHDGSPARPTTWDA
                     VAEVDPDLLLLMPCGLHLRETVDEWGRISPPEWIGELSAIQRGHLIALDGSSYF"
     gene            complement(901..1191)
                     /locus_tag="VHM48_12420"
     CDS             complement(901..1191)
                     /locus_tag="VHM48_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2756264.1"
                     /translation="MTYVIAEPCVDVLDLSCVSVCPVDCIHYEEGTDRKLFIDPNECI
                     DCGACEPECPVNAIFPEESLPAEWAAYTKADAVWFTDKAAARAMVDELKPSA"
     gene            complement(1278..1796)
                     /locus_tag="VHM48_12425"
     CDS             complement(1278..1796)
                     /locus_tag="VHM48_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756265.1"
                     /translation="MLDDIRLRVASAVVAAVVAITIGSCEAGPPPPTPPIVPGSSATP
                     REVNIVMKDWVFLPDPVDVAPGETVLLHVVNGGLIVHEAVIGDQSVQDAWEAAEGAAA
                     NPPPGPTPLVSVPPDVAGIRVVVPSGQRVDVVWTVPADPATVRALILGCHIPEHWAKG
                     MRAAIRLAPGQG"
     gene            2029..3126
                     /locus_tag="VHM48_12430"
     CDS             2029..3126
                     /locus_tag="VHM48_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756266.1"
                     /translation="MPAVRLEGLTKTFGDNATGRRVAQSGAGTPGVFPLVAVDHVDLD
                     VRDGEFFSMLGPSGSGKTTTLRMIAGFELPTEGRVLLHGVDVTREPPFARDVNTVFQD
                     YALFPHMSVGDNVAYGLLVRKVPKAERTRRVGEALDMVRLNGYERRKPGQLSGGQRQR
                     VALARALVNRPRVLLLDEPLGALDLKLREEMQIELKAIQQQVGITFIYVTHDQEEALT
                     MSDRLAVFNRGRIEQVGPPADVYERPASAFVAGFVGTSNLLRDDVARAIVGRPGTFTV
                     RPEKIRLAEPSDPVGDDETSALGHIRSVVYLGPDTRFLVTLDAGSELVVTQQNLVTSS
                     MDALALSGKAVRLIWKQQHNFAIESPGGGEA"
     gene            3123..4412
                     /locus_tag="VHM48_12435"
     CDS             3123..4412
                     /locus_tag="VHM48_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756267.1"
                     /translation="MRSRSLFAVAASLGIVVAACSSGATPAPASPSTAPASVAPSTAA
                     GSVAPPSVTPSSNLPTAIGAGEGELDIIVWAGYAEDGSNVKEYDWVHPFIAANADCAK
                     VNVKSADTSDSMVSLMAGGGGGLYDGVSASGDASNTLIARGDVAEVNVDLIPDFKDIT
                     PFLQSPAHNTVDGKHYGVSHGWGGNTLMWRTDKVTPAPTSWDVTFDPAKMEAYKGKIT
                     AYGGSIYVADAAVYLKTHSPDLGITDPYELTQPQFDAAIALLTAQHPFIGKYWEAYTD
                     EIDNFTTGATVVGTTWPYQTNTLKGQGVKVDAVVPSEGMTGWADTWMLASKAKHPNCM
                     YKWMAWMVTPHVQTQVAEYFGEAPANPKACAELDKVYASYGVKDFCTLYSVNDKAFYD
                     SISFWKTPQADCGDSRGQTCVSYDKWVTAFQAVKAGS"
     gene            4474..5406
                     /locus_tag="VHM48_12440"
     CDS             4474..5406
                     /locus_tag="VHM48_12440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756268.1"
                     /translation="MTATTPNAPPPPTPAAATIPAGLRRRMLAALYRRPRAQILLLLT
                     PPAGWFGVVYLGSLALLLVTAFWTFDGATGKVVQHWTLDNFGQLISQPAFRTVAQRTL
                     LFAVAVTVADMVIAFPIAYFMAQVASRRTRAVLFMLCLLPLWASYLVRVYAWRVILAP
                     NGPLDWTLGLFGLGPAGLYPSDLGVGIVFTYLWLPYMILPLYAGLERVPRSLLEASSD
                     LGARGATTFRRIVLPLVLPAIAAGSIFTFSLTLGDYITPGLVSNNQFIGNIIFEQQGV
                     SGNLPLAAAFALVPVAIMAVYLFVARRLGAFEAL"
     gene            5406..6191
                     /locus_tag="VHM48_12445"
     CDS             5406..6191
                     /locus_tag="VHM48_12445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756269.1"
                     /translation="MGRWLLRAATGAVLAFLYLPIATIALYAFNAQRIQVWPIREFSA
                     QWFGAAWDNPSVRDALALSVQVGLVSALIAIVLGSLAAFAIHRFRFFGREIFSLSLVL
                     PIALPGIVTGLALNATVRTAGISFSTATIVVGHATFCIVVVYNNVVARLRRSSRNIEE
                     ASMDLGADTWQTFRFITLPVIRTAVLAGGLLAFALSFDEIVVTTFTAGTQETIPIWIF
                     NHLRQANQRPIVNVVALILILLSTIPVYLASRLTTSNETGGRT"
     gene            complement(6421..6591)
                     /locus_tag="VHM48_12450"
     CDS             complement(6421..6591)
                     /locus_tag="VHM48_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX2756270.1"
                     /translation="MPIEKDPVCGMEVDTTATDLKLEHDGTTYWFCGKGCFLEFRDDP
                     DRFLAEDYQPSM"
     gene            6692..7033
                     /locus_tag="VHM48_12455"
     CDS             6692..7033
                     /locus_tag="VHM48_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX2756271.1"
                     /translation="MTDPLLHDPRPALVAERDRLRAGIAEGIVAPDPMTYGSQAAAAS
                     QVFEQQRDLALRDHNEQHLEAVEAALARLDDGTYGRCTRCGRLIAPERLDALPWAAHC
                     IDCQKIVGRGG"
BASE COUNT         1070 a         2557 c         2348 g         1089 t
ORIGIN      
        1 gaagtacgac gagccgtcga gcgcgatcag gtggccgcgc tggatcgccg acagctcgcc
       61 gatccattcc ggcggggaga tccggcccca ttcgtcaacg gtctcgcgga ggtgcaagcc
      121 gcacggcatc aggaggagca ggtccgggtc gacctcggcg accgcgtccc aggtcgtcgg
      181 ccgggccggg ctgccgtcgt ggccgaggac gtcccagccg ccggctcgcc ggatctggtc
      241 cggcacccag tggccgaccg cgaacggcgg atcgagccat tcgaggccga cgacgcgcgg
      301 cccgacgaac ccgcgctccc gtcgctcctg ggctttcgat tcgatcgccc cgagccgcga
      361 ccggaggctc tcgacgagct cgatcgccgc gtcctcggcc tcggccatcg cgccgaccgt
      421 cgtgatcgtg ttgaggatcc cctcgaccga tgtcggctcg agcgagacga ccgtcgcgtc
      481 catctcgagg gcccggaccg cctcgctgac ctggcgatac ccgacggcgc agaccgcgca
      541 cagctcctgg gtcaggatca ggtccgcgtc ggcgacttcg agcgcgtcct cgtcgaggtg
      601 gtagatcgcc gagccgccgt ggaccgcatc gccgacgcgg cgatggatct cgcggctcga
      661 ggcgccgacc aggtcgaggg cgttgctggt catgaccggc aggccgacga cctcgggtgg
      721 ccagtcgcat tcgtgggtca cgcccacgag ctcgtcgcgc aggccgaggg cgcacacgat
      781 ctcggtcgcg gacggcagca gggagacgat cctcatcgat ctgtgccgtt gggaaccggc
      841 aacgtcagcg ttcagccgcc tctcgctcac cgaggcagga ggccgagccc cgcgaggcca
      901 tcaggctgat ggcttcagct cgtcgaccat cgcccgggcg gccgccttgt ccgtgaacca
      961 gaccgcgtcg gccttcgtgt acgccgccca ttcggccggc agcgactctt cggggaagat
     1021 cgcgttcacc gggcactctg gctcacaggc accgcagtcg atgcactcgt tcgggtcgat
     1081 gaacagcttc cggtcagtgc cttcctcgta gtggatgcag tcaaccgggc agaccgagac
     1141 gcacgacagg tcgagcacgt cgacgcacgg ctcggcgatc acgtaggtca tgggattggt
     1201 tctcctcgac ccgctcagtg gagattggtc ggtgcgccgt gtcggcgggc ccgaatccta
     1261 gcaccgcagc gcgattgtca gccttgcccc ggcgcgagcc ggatggcggc ccgcattccc
     1321 ttcgcccaat gctccggaat atggcagccg aggatgagcg cgcggacggt tgccggatcg
     1381 gccgggaccg tccacacgac atcgacgcgc tggcccgatg ggacgacgac ccggatcccg
     1441 gcgacgtccg gcgggacgct gacgagcggt gtcgggcccg gcggcggatt ggcggccgcg
     1501 ccctcggccg cctcccaggc atcctggacc gactggtcgc cgatgaccgc ctcgtggacg
     1561 atcaggccgc cgttcacgac gtggaggagg accgtctcgc cgggcgcgac gtcgaccgga
     1621 tccggcagga agacccagtc cttcatcacg atgttgacct cacgcggcgt ggccgacgag
     1681 cccggcacga tcggcggcgt cggcggcggc gggcccgcct cgcagctgcc gatggtgatc
     1741 gcgacgaccg cggccacgac ggcggaggcg acacgaagcc ggatgtcatc gagcacgggc
     1801 ggcagtctag ggccgccgtc cgaccgtcgt gcgcgacgca gccggtcgcg ccttgacacg
     1861 cgagcgagcc gaacggcaga gtgcggcatc gcttcgcccc ggcggagcac cgtcatccgc
     1921 gtccccgcga ccccctccgg gcccagaccg aggaaccgct tggcgctccc gtcaccgacc
     1981 gccgccgtcg acacatcacc accggctgtc gccagccacg gcggcgccgt gcctgccgtc
     2041 cggctcgagg ggctgaccaa gaccttcggc gacaacgcca ccggccgccg cgtggcccag
     2101 tcgggtgccg ggacccccgg cgtctttccc ctcgtcgcgg tcgatcatgt cgatctcgat
     2161 gtgcgcgacg gcgagttctt ctcgatgctc gggccgtccg ggtcgggcaa gacgacgacc
     2221 ctccggatga tcgccgggtt cgagctgccg accgagggcc gggtcctcct ccacggcgtc
     2281 gacgtcaccc gcgagccgcc gttcgcccgg gacgtgaaca ccgtcttcca ggactacgcg
     2341 ctgttccccc acatgtcggt cggcgacaac gtggcctatg ggctcctcgt acggaaggtc
     2401 cccaaggccg agcggacgcg ccgggtcggc gaggcgctcg acatggtccg cctcaacggc
     2461 tatgagcggc ggaagcccgg tcagctgtcc ggcggccagc gtcagcgggt ggcgctcgcc
     2521 cgggccctgg tcaaccggcc gcgcgtcctc ctgctcgacg agccgctcgg cgccctcgat
     2581 ctcaagctcc gcgaggagat gcagatcgag ctcaaggcga tccagcagca ggtcgggatc
     2641 accttcatct acgtcaccca tgaccaggag gaggcgctga cgatgagcga ccgcctggcc
     2701 gtcttcaacc gcggccggat cgagcaggtc gggccacccg ccgacgtcta tgagcgcccg
     2761 gcgtcggcgt tcgtggccgg gttcgtcgga acgtccaatc ttctccgcga cgacgtcgcg
     2821 cgggccatcg tgggacggcc cgggacattc accgtccggc cggagaagat ccgcctggcc
     2881 gagccgtcgg acccggtcgg cgacgacgag acatccgccc tcggccacat ccggagcgtc
     2941 gtctatctcg gccccgacac gcgattcctc gtcacgctcg atgccggctc ggagctcgtg
     3001 gtcacccagc agaacctggt cacatcatcg atggacgccc tcgccttgag cggaaaggcc
     3061 gttcgcctca tctggaagca gcagcacaac ttcgcgatcg agtcacctgg aggaggagaa
     3121 gcatgagatc ccggtcactg ttcgccgtgg cggcgagcct cggcatcgtc gttgcagcgt
     3181 gttcgagcgg ggcgacgccg gcgccggcaa gtccatcgac cgctccggcg agcgtcgccc
     3241 cgagcacggc cgccggctcc gtcgcgccgc catcggtcac gccgagctcg aacctcccga
     3301 ccgcgatcgg agcgggcgag ggcgaactcg acatcatcgt ctgggccggc tatgccgagg
     3361 acggctcgaa cgtcaaggaa tacgactggg tccacccgtt catcgcggcc aacgcggact
     3421 gcgccaaggt caacgtcaag tcggccgaca cgtcggactc gatggtctcg ctcatggccg
     3481 gcggcggtgg cggcctgtac gacggcgtct cggcctcggg cgatgcctcg aacaccctga
     3541 tcgcgcgggg cgacgtggcc gaggtcaacg tcgacctgat ccccgacttc aaggacatca
     3601 ctccgttcct ccagtcgccg gcccacaaca cggtcgacgg gaagcactac ggcgtctccc
     3661 acggctgggg cggcaacacc ctgatgtggc ggacggacaa ggtgaccccg gccccgacga
     3721 gctgggacgt cacgttcgat ccggccaaga tggaggcgta caagggcaag atcaccgcgt
     3781 acggcggatc gatctacgtc gcagacgcgg ccgtctacct caagacgcac tcgccggacc
     3841 tcgggatcac cgacccgtac gagctgaccc agccccagtt cgacgcggcg atcgccctgc
     3901 tgacggccca gcacccgttc atcggcaagt actgggaggc gtataccgac gagatcgaca
     3961 acttcaccac cggtgcgacg gtcgtcggca cgacgtggcc gtaccagacg aacaccctca
     4021 aaggccaggg cgtcaaggtc gacgcggtcg tcccgtccga agggatgacc ggctgggcgg
     4081 acacgtggat gctcgcctcg aaggccaagc accccaactg catgtacaag tggatggcct
     4141 ggatggtcac gccgcacgtc cagacccagg tcgccgagta cttcggcgag gccccggcca
     4201 atcccaaggc ctgcgccgag ctcgacaagg tctatgcctc gtacggcgtg aaggacttct
     4261 gcacgctcta cagcgtcaac gacaaggcct tctacgactc gatcagcttc tggaagacac
     4321 cccaggccga ctgcggcgac agtcgcggcc agacctgcgt ctcgtacgac aagtgggtga
     4381 ccgcattcca ggcagtcaag gccggcagct gaccgatcca tcgcagacgc ccggccatcc
     4441 ggccgggcgt cgattcgcca gggagccgag gcgatgaccg cgacgacgcc caacgctccg
     4501 ccaccgccga ccccggccgc ggccacgatc ccggctggct tgcggcggcg catgctcgcg
     4561 gccctctatc gccggccgcg ggcccagatc ctgctcctgc tcacgccgcc ggccggctgg
     4621 ttcggcgtcg tctatctcgg ctcgctcgcg ctgttgctgg tgacagcgtt ctggaccttc
     4681 gacggggcca ccggcaaggt cgtccagcac tggacgctcg acaacttcgg gcagctgatc
     4741 agccagcccg cgttccggac cgtcgcccag cggacgctcc tgttcgcggt ggcggtcacc
     4801 gtcgccgaca tggtgatcgc gttcccgatc gcgtacttca tggcccaggt cgccagccgg
     4861 cgaacccggg cggtcctgtt catgctgtgc ctgctccccc tctgggcaag ctacctcgtc
     4921 cgggtctacg cctggcgggt catcctggca ccgaacgggc ccctcgactg gacgctcggg
     4981 ctgttcgggc tggggccggc cgggctgtat ccatccgatc tcggggtcgg gatcgtcttc
     5041 acctatctct ggctgccgta catgatcctg ccgctttacg ccggcctcga gcgcgtgccg
     5101 cgctcactcc tcgaagccag ctcggacctc ggcgcccgcg gcgcgacgac cttcaggcgg
     5161 atcgtcctgc cgctcgtcct gccggccatt gcggcgggct cgatcttcac gttctcgctg
     5221 accctcggcg actacatcac gcccggcctc gtctcgaaca accagttcat cgggaacatc
     5281 atcttcgagc agcagggcgt atcggggaac ctgccgcttg cggcggcctt cgcgctggtg
     5341 ccggtcgcga tcatggccgt ctacctgttc gtcgcccggc gtctcggcgc cttcgaggcg
     5401 ctctgatggg tcgctggctg ctgcgcgccg cgacgggagc cgtcctcgcc ttcctgtacc
     5461 tgccgatcgc gaccatcgcc ctctacgcct tcaacgccca gcgcatccag gtctggccga
     5521 tcagggaatt cagcgcccag tggttcggtg ccgcctggga caacccgtcg gtccgcgacg
     5581 cgctcgccct gtcggtccag gtggggttgg tctctgccct gatcgcgatc gtcctcggct
     5641 cgctggccgc cttcgccatc caccggttcc ggttcttcgg ccgcgagatc ttctcgctgt
     5701 cactggtcct gccgatcgcc ctgccgggca tcgtcaccgg cctggccctg aacgcgaccg
     5761 tgcgcaccgc cgggatcagc ttctcgacgg cgacgatcgt cgtcggccac gccacgttct
     5821 gcatcgtcgt cgtctacaac aacgtcgtcg cccgactcag gcgctcgtcg cggaacatcg
     5881 aggaggcctc gatggatctc ggggcggaca cgtggcagac gttccgcttc atcacgctgc
     5941 cggtgatccg gacggccgtg ctcgccggcg gtctgctcgc gttcgcgctg tcgttcgacg
     6001 agatcgtcgt cacgacgttc accgcgggga cgcaggagac gatcccgatc tggatcttca
     6061 accatctgcg ccaagccaac cagcgcccga tcgtgaacgt cgtggccctg atcctgatcc
     6121 tgctctcgac gatccccgtc tacctcgcct cgagactcac gacgtccaac gagaccggcg
     6181 gacgaacctg acgacgatca gtgccagcgc tgtccggcct ggccggcgag ggcgatcgcg
     6241 gcgagcgaga cgatcggggc gagaatccgg acaagggcgt tgaggcgatg gcgcatcgtg
     6301 ggcactcctc ggcgatggcc ggccgacggc aggcgatcga ccccgtctga cggagtgccc
     6361 ggtccctgga tcgctgccgg cagtgtcgcc ggcgccgatt agctgcgact taccccgcgg
     6421 tcacatcgac ggctggtagt cctcggcgag gaaccggtcg ggatcatccc ggaactcgag
     6481 gaagcagccc ttcccgcaga accagtaggt cgttccgtcg tgctcgagct tgaggtcggt
     6541 tgcggtcgtg tcgacctcca tcccgcagac aggatccttc tcgatgggca tgtggacaac
     6601 tcctcgaaga tggcacggga cggtgggtcc tgaggatacc gaagccgcgg ttcgccgacc
     6661 gacggtcccg ccatcggcgt acgatcgggt ggtgaccgat ccgctgctcc acgacccgcg
     6721 cccggccctc gtcgccgagc gcgaccgcct gcgcgccggc atcgccgaag ggatcgtcgc
     6781 gccggacccg atgacctacg ggtcgcaggc cgccgccgcc agccaggtct tcgagcaaca
     6841 gcgcgacctg gcgctccgcg accacaacga gcagcacctc gaggcggtcg aggccgctct
     6901 cgcccggctc gacgacggga cgtacgggag gtgcacccgc tgcggccgcc tgatcgcccc
     6961 ggagcggctg gacgccctgc cgtgggcggc ccactgcatc gactgccaga agatcgtggg
     7021 ccgcggcggg tgagcgtgga cgaggcagcg accggcgccg cggc
//
LOCUS       DATAIW010000416         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_191180, whole genome shotgun sequence.
ACCESSION   DATAIW010000416 DATAIW010000000
VERSION     DATAIW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191180"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1063)
                     /locus_tag="VHM48_12460"
     CDS             complement(86..1063)
                     /locus_tag="VHM48_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2756272.1"
                     /translation="MQTSRAFRGLDDRTLNRAIKIMVLTLVIGVPTIAVLYWMDRHVD
                     AAPAMADRTVAAAEEAVRTKPDDLATRNHLAAAYVTADRYADGIAQFSQVLTSDSKNR
                     PALLGRGIALITTKQYDAAAADFQAIVDGSKGGEFAATDPQLQQAYYELGVIALEQQR
                     PADAIAPLERALKINSADADALYRYGMALIGTGDPKTGVIAIRRAVMFVPSGWCEPYA
                     GLVTGYTALADQPGIGYANGMVAFCAGRLDEASTTLGALTQGPKKIDALLGLALVSAT
                     RGDATAATGLYQQVLAEEPSNASALIGLGQLGGSNAHASQPAASPAGSN"
     gene            complement(1331..1732)
                     /locus_tag="VHM48_12465"
     CDS             complement(1331..1732)
                     /locus_tag="VHM48_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756273.1"
                     /translation="MDTPSVRLAILYDHPLLGEGVGRMLGLEPGLDIEYVEANDRAGV
                     NAAIAALPDMVVVERTSGIDPLEILRRTPATLVIEVCIGPGPTWVYRRQEIPGEPQAV
                     LGLVQRLRGGHPSVAFEPADAERHLTGSPAA"
     gene            complement(1878..2522)
                     /locus_tag="VHM48_12470"
     CDS             complement(1878..2522)
                     /locus_tag="VHM48_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756274.1"
                     /translation="MLVEDQPLFRTALATLIGDQPDFTVVGQAENGLQALELARELEP
                     DVVLMDVEMPVMDGIAAVHLIKEQFPAVRVIMLTVSESDDHLFDAIRFGADGYLLKDL
                     RPEHLYDMIRAVMRGETPLSPAIAGRLLRDVRERRPIHVAPTVEEQTLTRREIETLQL
                     VATGMSNKEIGVRLSITEGTVKNHVHNALEKLQLENRIQAAAYVVRQGWAVRPS"
     gene            complement(2512..4311)
                     /locus_tag="VHM48_12475"
     CDS             complement(2512..4311)
                     /locus_tag="VHM48_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2756275.1"
                     /translation="MSGRLPLSDSVPDESNLRGLKLLAVFLPVTAVIVGEIGRAILIA
                     PSVGTDLDHLISAGLAIAAVLAFTGLMLAGIGRAQRSLIRQNRDLRLATAVSVDLQED
                     DPLDEVIQRALTTLMDATGAREASVEILADPQTKGVARTIRRATDDAPPVNPTSHLEI
                     PLRSGTALVGQLWIGPADHGATGPVDLGVVELIAHEIASAIQVRQLVVDLRRRRRQSS
                     ALYDIALQVTNRQPLADILGSISLHAHDLLEVDEATLCLSESASAALRQAHVLETDPP
                     TGDGLTCFIRSAAGPIESLHSKSPICPVKAARTWGNIAQAPLRSSDATLGELWVARQR
                     GEPFDDLDRDLLAGLADLAAIAVVSADLRQRDAMAAIVAERERIAREMHDSLAQVLAT
                     SHLRLRALEGRSEVRDHAALAAEIAALGDLTHEAYVDVREAILGLRESSHAERDLLGS
                     LRIYLEKFSHQTGLAGRLETELPDDLGLAPGAEIQVIRVIQEALTNIRKHAGATRAVV
                     RVVGDELGICLSVDDDGRGFDPKELGGREDGFGLHAMRERMNLVGGTLMIYSAPGEGT
                     RVVARLPQAAGITAPASRPLHDDRHESYANIAG"
BASE COUNT          697 a         1606 c         1747 g          772 t
ORIGIN      
        1 gcccgggggc gattcgagct tcggcccggg gtccgtgatg acgtccccgg gcagggtgag
       61 atcggtcatc gtggcgtcgg tcatgtcagt tgctcccggc cggcgaggcc gccggctgag
      121 aggcgtgggc gttcgagccg ccgagctggc ccagcccgat gagggccgat gcgttcgatg
      181 gctcctcggc caggacctgc tgatacagcc cggtcgccgc ggtcgcgtcg ccgcgggtgg
      241 ccgacacgag ggcgagcccg aggagggcgt cgatcttctt cggcccctgg gtcaacgccc
      301 cgagcgtggt cgacgcctcg tcgaggcgtc cggcgcagaa ggcgaccatc ccgttcgcat
      361 agccgatgcc gggctgatcg gcgagggcgg tgtacccggt caccaggccg gcgtacggct
      421 cgcaccagcc cgacgggacg aacatgacgg cccgccggat ggcgatcacc ccggtcttcg
      481 ggtcgcccgt cccgatcagc gccatgccgt agcgatagag cgcatcggcg tcggcgctgt
      541 tgatcttgag ggcccgctcg agcggcgcga tcgcgtccgc cggtcgctgc tgctcgaggg
      601 cgatgacgcc cagctcgtag taggcctgct ggagctgggg gtcggtggcc gcgaactcgc
      661 cacccttcga cccgtcgacg atcgcctgga agtcggcggc tgccgcgtcg tactgtttcg
      721 tcgtgatgag cgcgatcccg cggccgagga gggcggggcg gttcttcgaa tcgctcgtca
      781 ggacctggct gaactgggcg atcccgtccg cgtagcggtc ggcggtgacg taggccgcgg
      841 cgaggtgatt ccgggtggcg agatcgtcgg gcttggtcct gaccgcctcc tcggcggccg
      901 ccacggtccg atcggccatc gccggggcgg catcgacgtg gcggtccatc cagtacagga
      961 ccgcgatcgt cggcacgccg atcaccaggg tgaggaccat gatcttgatc gcgcggttga
     1021 gggttcggtc gtccaggccc cggaaggccc gcgatgtctg catctggaat gtcagtcctt
     1081 cgcgtcgggt gttcaggccg gttctccgcc tcgaccgaag ggatcaggtc gagacggagg
     1141 cccggtcctc gcccgatcgt ggactgtcga gcccgcggtt cccatccgcc gacgggtaga
     1201 tatcgggtac acgtccggtt cgcgacgtca taggaccgac gtcatggctc cgacgccgag
     1261 gatcgacgtg aggagcccgc ggtttgacga gctgcggtcc ggttcgcgct ggatccggag
     1321 ccgggccggg tcaggcggcc gggctgccgg tgaggtgccg ctcggcgtcg gccggctcga
     1381 acgcgaccga cgggtgcccg ccacggagcc gctggacgag gccgaggacg gcctggggtt
     1441 cgccggggat ctcctggcgc cgatacaccc acgtcggacc ggggccgatg cacacttcga
     1501 tcacgagcgt ggccggcgtc cgacggagga tctcgagcgg gtcgatcccg ctcgttcgct
     1561 ccacgaccac catgtcggga agcgcggcga tggccgcatt gacccccgcc cggtcgttcg
     1621 cctcgacgta ctcgatgtcg agacccggct ccaggccgag catccgtcca acgccctcgc
     1681 cgaggagcgg gtggtcgtac aggatggcca gacggacgga cggcgtgtcc aaggcgagct
     1741 ccaatgggtt cgaatgccgc gatcgtatgg tggtcgatcg tcgagcacca tccccggacc
     1801 gacacagacg aagctgcccg tcggcctatc gacggatacc ctgccggctc cggctcgatc
     1861 tggagcgcga cgccccgtca ggacgggcgg acggcccagc cctgccgcac cacgtaagcc
     1921 gcagcctgga tccggttctc gagctggagc ttctcgaggg cgttgtgaac gtggttcttg
     1981 acggtcccct cggtgatcga gaggcggacg ccgatctcct tgttcgacat cccggtcgcg
     2041 acgagctgga gcgtctcgat ctcgcgcctg gtcagcgtct gttcctcgac cgtcggcgcg
     2101 acgtggatcg ggcgccgctc gcggacgtcg cggaggaggc gtccggcgat ggccggggac
     2161 agcggcgtct cgccgcgcat gacggcccgg atcatgtcgt agaggtgttc cgggcggaga
     2221 tccttgagca ggtagccgtc ggcgccgaac cggatggcat cgaagaggtg gtcatccgac
     2281 tcggagaccg tgagcatgat cacgcgtacc gccgggaact gctccttgat gaggtggacc
     2341 gcggcgatcc cgtccatgac cggcatctcg acatccatca ggacgacgtc gggctcgagc
     2401 tcacgggcca gctcgagcgc ctggaggccg ttctcggcct ggccgaccac cgtgaagtcc
     2461 ggctggtcgc cgatcagcgt cgcgagagcg gtccggaaca gcggctggtc ttcaaccagc
     2521 aatattcgcg taggactcgt ggcggtcatc atggagaggc ctcgatgctg gggccgtgat
     2581 cccggccgcc tggggcaggc gggcgaccac ccgcgtcccc tcccccgggg cactgtagat
     2641 catgagcgtg ccaccgacga ggttcatgcg ctcgcgcatc gcgtgcaggc cgaagccgtc
     2701 ctcgcgcccg ccgagctcct tgggatcgaa gccgcgaccg tcgtcgtcca cgctgaggca
     2761 gatgccaagc tcatcgccga cgacgcgcac gacggcccgg gtcgcaccgg catgcttgcg
     2821 gatgttcgtg agtgcctcct ggatgacccg gatgacctgg atctccgcgc cgggcgccag
     2881 gccgaggtcg tcggggagct ccgtctcgag gcggccggcg aggccggtct ggtggctgaa
     2941 cttctcgagg tagatgcgga ggcttccgag caggtcccgt tcggcgtggc tggactctcg
     3001 cagaccgagg atcgcctccc gaacgtcgac ataggcttcg tgggtcaggt cgccgagggc
     3061 ggcgatctcg gccgcgaggg cggcgtggtc gcggacctcc gaacgccctt cgagagcgcg
     3121 cagccggagg tggctggtcg cgagcacctg ggcgaggctg tcgtgcatct cccgggcgat
     3181 tcgctcgcgc tccgccacga tcgcggccat cgcgtcacgc tgtcgaaggt cggcgctcac
     3241 gacggcaatg gccgcaagat cggccagccc cgcgaggagg tcccgatcga ggtcgtcgaa
     3301 cggctccccc cgctgccgtg ccacccacag ctcaccgagc gtcgcgtcgg agctccgcag
     3361 cggcgcttgc gcgatgttgc cccacgttcg tgccgccttg accgggcaga tcgggctctt
     3421 cgagtggaga ctctcgatgg ggccggcggc ggaccggatg aagcaggtga gcccgtcacc
     3481 cgtcggcgga tcggtctcga ggacgtgcgc ctggcgaaga gcagccgacg ccgactcact
     3541 gaggcagagg gtcgcctcgt ccacttcgag gaggtcgtgg gcatggaggc tgatcgaccc
     3601 gaggatgtcc gcgagcggct gtcgattcgt cacctggagc gcaatgtcgt agagcgcgct
     3661 cgactggcgc cgccgccgcc gcagatcgac gacgagctgg cgcacctgga tcgcactggc
     3721 gatctcgtgg gcgatcagct cgactactcc gagatcgacc ggcccggtcg cgccgtggtc
     3781 cgcaggtccg atccagagct gacccacgag ggcggtcccg gaccgtagcg ggatctcgag
     3841 gtgactggtc gggttcaccg gcggggcgtc atcggtggcg cggcggatcg tccgggccac
     3901 gcccttggtc tgggggtcgg cgaggatctc gacggacgct tcccgggccc cggtcgcgtc
     3961 catcagcgtt gtgagcgctc gctggatcac ctcgtcgagc gggtcgtcct cctggagatc
     4021 gaccgaaacg gccgtcgcga gccggaggtc gcggttctgc cggatcagcg agcgctgagc
     4081 ccgcccgatg ccggcgagca tcagccccgt gaacgccagg acggcggcga tggccaggcc
     4141 ggcggagatg aggtggtcga ggtccgtccc gaccgacgga gcgatcagaa tcgcccgacc
     4201 gatctcgccg acgatcaccg cggtgacggg caggaagacg gccagcagct tcaggccgcg
     4261 caggttcgac tcgtcgggca ccgaatcgga caacggcaga cgaccgctca tctcaggcca
     4321 ggggctccct tcaggcgagc gctgatcgct cgacgccctt accttcggtc acgtcgatgg
     4381 ccccgacacc tgcccattgg gctcccgccc aagggccata cagcccgtag cgggcgggcc
     4441 gccccgatcg gcccccgtgc gctcccgccg gcgaacgcgg tcaggttgac atgggcgtgg
     4501 cgacgctggc gctcttgatc ctggtcgggt gcggcccatc gaagggatcg cgcgcccacg
     4561 agagcgaagc gtccggcggc ctgtgctgca ctgccgtcgg ttcgaccgat gtcgtctatg
     4621 tggtcgcaaa gtgggttcgg cctctcggac agcatcactg ggtacgtctg gggtgaggcc
     4681 acaggtggat ccgcttgccc gtcgtcaaac tccggatcct tctacgactg ggagaagtac
     4741 gacagagcgt gggctggacc tatgtcacga gctcaaccgt gtatcggtat tggtccagta
     4801 tggtcggagc gccgtgctgg ac
//
LOCUS       DATAIW010000417        10659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_28270, whole genome shotgun sequence.
ACCESSION   DATAIW010000417 DATAIW010000000
VERSION     DATAIW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28270"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM48_12480"
     CDS             <1..318
                     /locus_tag="VHM48_12480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2756276.1"
                     /translation="VAARSGGFLYCVSLVGVTGARAELPATVAELVRDVSAVSPVPVG
                     VGFGVSQAAHVRAIAATGAGGVIVASALVDALGEDGTDVAAMERLVRDLAAATRTSVD
                     ALI"
     gene            383..1084
                     /locus_tag="VHM48_12485"
     CDS             383..1084
                     /locus_tag="VHM48_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756277.1"
                     /translation="MLFVDRLRPIRQATQVLRVAAHVARRQRTLERAIRNMPDPVPWD
                     WAAPRLMPLLSGPQFDDPELPIVRMTSELGPAIEFGLDLGGVFLTVDRRVAERWECSA
                     EQLLERGLKNLRDRAAKIAPDQVVAGVMSGRTIRVLRDRPTWASSTLLDLPTIHRLFG
                     AHDQLLAAPTTGCVVSLPIDTPTRTAADIVIDFEGPLTSLFLDPFVMEDGTLLWGGDV
                     SADDLDDDCGEPWTS"
     gene            1109..1354
                     /locus_tag="VHM48_12490"
     CDS             1109..1354
                     /locus_tag="VHM48_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX2756278.1"
                     /translation="MSTRTTLTLDDDVADRLAREARRSGRSYRAVVNAALRRGLDTVI
                     DASEPFRLNSAEMRRRPEFDLDHIEDLLDRLDGPQRR"
     gene            1357..1782
                     /locus_tag="VHM48_12495"
     CDS             1357..1782
                     /locus_tag="VHM48_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEX2756279.1"
                     /translation="MIDANLLLYAYDEADPRHPVVARWFQSLMSSSDDVGLPLLAILA
                     FIRLSTDHRVFEQPRSVATALAIVESCLSRPNVRLLEPTGGHWATLADLAARGQARGP
                     QLMDAHLAALTIEHGATLATADRGFARFPGLRTLDPTAA"
     gene            1841..2500
                     /locus_tag="VHM48_12500"
     CDS             1841..2500
                     /locus_tag="VHM48_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756280.1"
                     /translation="MTKPILVTIEHTAGKTFATAADWPGWSRSGKTEELALGALVAYA
                     PRYAVIADADHRRIAAGPTIEDLDVVERNAGSAGTEYGVPSRPSDHDARPTEADDAKR
                     LAGLVEVAWVFFDRTAAAAPEELRKGPRGGGRNTSKVIAHVMDADRAYANEIGIKVRE
                     FAPDDRAAIAAMRAAMLEVLRAARDGTPLAGRRWPARYAAHRIAWHALDHAWEIEDRT
                     E"
     gene            2521..3120
                     /locus_tag="VHM48_12505"
     CDS             2521..3120
                     /locus_tag="VHM48_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756281.1"
                     /translation="MASRSKIEPNRPGDALIGMEAVLDQSPPTLAHLVAAARRGDRDA
                     FRALVEPDLPAALGTARIVTRSEADASDAVQEALLSAWRGLDGLREPEAFRAWFRRHV
                     VRAALRAASRRGRVVELDLTASAPEGELDRALEQRTLGRAFARLDPPDRLLLTLHHFW
                     GLPIAETAAHLDIPEGTVKSRVHYAMDRLRAAYAAEERR"
     gene            3117..4478
                     /locus_tag="VHM48_12510"
     CDS             3117..4478
                     /locus_tag="VHM48_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756282.1"
                     /translation="MSDDFEDLLKRWLRDHGATDRSAIEAVAGHVAVLPPRRRRQLGP
                     LAVAAAVIVAIGLAAFALVPRQSSVTSQPSSPVPPDPAAFAGDARLARCGASVDTALD
                     VFEMGRARDYRLHLPAMGLSPELDVDTPGFVVVFRDMQPFAVLGAPPPAGQTWPPRSL
                     APGHHDVCILVGADPATAELNIYDDVDITGLAAFVAGTDPSTSSGPDATASVAASPSI
                     EPSLTPEPAPAWVDRLAGQLECTGEVANIGDEVSGTAGTDPLGPTAAAALTSFLGPTN
                     AYASLPAGGFTQIHVDIHWASFGHLVGGRLKAVIVLTDISDFGPGWVVRGIRACDASE
                     FDPAVPLTFPVTIWTDSSGARVSTDLIRSMAGPAHCGWDTVTFLQFGGDLYFRDPKGA
                     MNAWTTTSFEPRALIPRNAEASGYRSGGAALWTVPGGDAYLDFSGHVERWPRSTDSTI
                     GCM"
     gene            complement(4546..4911)
                     /locus_tag="VHM48_12515"
     CDS             complement(4546..4911)
                     /locus_tag="VHM48_12515"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2756283.1"
                     /translation="MAERQGPGAGPDEPAELRRLRRRIDAVDRRIVRLLNDRAELARE
                     VGRVKATTGLRAIRDPLREREVLLRVSMANDGPTPQADLLAIFKRLFVIARSLERRDR
                     NRERRAAPFGGVPPTAEDD"
     gene            complement(4904..5347)
                     /locus_tag="VHM48_12520"
     CDS             complement(4904..5347)
                     /locus_tag="VHM48_12520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX2756284.1"
                     /translation="MTSVLVLQGPNLNLLGSREPEIYGHDTLDDIHAEIAARAVELGL
                     DADFFQSNHEGALIDRLHRRDFDVVIVNAGGLTHTSVSLRDALLGVQRPFWEVHLSDP
                     STREAFRSVNFLHDVAVASFVGQGKRGYLLALEAIAARFGEAGGG"
     gene            complement(5344..6993)
                     /locus_tag="VHM48_12525"
     CDS             complement(5344..6993)
                     /locus_tag="VHM48_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEX2756285.1"
                     /translation="MDVVLVGLPGSGKSAVGRRLAHRHGASFVDLDDVIEKSVGKTIP
                     EIFAERGEAAFRALERQAVADLGPADRGPAVRSVVATGGGAVIDPRNRWALYRGRVPV
                     WLDVRPEVLAQRLRRSPNVRPLMAGRDPMGAIRDLARERDRFYSAAQRMNGVAELASI
                     VERIDELVARAAREDEPSVLLRGHSLGGELVIGEGIATALIGDTLRGLRARRAILVSE
                     PGAWSAAGEAIGAALADDGWPVERILLPQGESAKRMAVVEEAASRLASLRAERGEPLV
                     AIGGGALGDAAGFLAASWLRGVPIIAVPTTLVAQIDSSIGGKTGVDLPEGKNLVGAFH
                     PPAAVVIDIAFLRSLPERQRRAALGEAVKMAALGDELLFALLERDGEAVARGDEAAFA
                     GGAIAELVERTAWAKVAVVDADEKEQGAADGRIRLNLGHSLGHAVEAAGGFGDLLHGE
                     AVAHGLRGAVRIGRELGVTPEDRGERIERLLTALGLATERLPYPLATVLGALAADKKH
                     ADGALRWVLPTADGSVVRADVPADVVERAASSLLAPPGGPS"
     gene            complement(7007..8302)
                     /gene="aroC"
                     /locus_tag="VHM48_12530"
     CDS             complement(7007..8302)
                     /gene="aroC"
                     /locus_tag="VHM48_12530"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2756286.1"
                     /translation="MGRLRWLTAGESHGRTLAVTVEGVPAGLPLGGDDLAIDLARRQR
                     GYGRGARQAIEQDRADIAGGVRHGMTLGSPILLLVANRDWDNWTRVMQVEGLSDDESA
                     ELAAAAADGNKRATPVTRVRPGHADLAGALKYGFNDVRNVLERASARETAARVAAGGV
                     ARAILGALGVEVWSFTAEVGGVAVDPARATRTRDEADASPLRCPDPAAEAAMIARIDE
                     ARSNGDTVGGVFEVVAHGLPIGLGSYVHWDRRLDAALAAAVMSINIVKGVEFGLGFEQ
                     TKRFGSAVHDVIAGRGEGGRWIHRSNNAGGLTGGITNGEPLVVRGAVKPISTLARPLP
                     SADLVSGEAVDKAHYERSDISVVPAAGVVAEAMVMLTLADAMLDKFGGDTIADTRDAL
                     DRYVARISGPIGAPVAGGRRGAVDPIAAGGDEAGSGGDD"
     gene            complement(8322..9236)
                     /gene="aroE"
                     /locus_tag="VHM48_12535"
     CDS             complement(8322..9236)
                     /gene="aroE"
                     /locus_tag="VHM48_12535"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX2756287.1"
                     /translation="MTKRVVLIGHPVAHSLSGAMHQAAFDDQGIDAAYELWDRAPIAL
                     PDAIGEIRGDEFLGANVTIPHKERVVPLVDRLTEDAQATGAVNTITREGKRLVGHNTD
                     VPGFKIALDKLVGRQKMPRHAIVLGAGGGSRAVVFGLIREGFQRIIVFNRHLHRAEAL
                     VKHFGRSAAHMELRAMPWHESIIESELAKSKVLVNATSIGLTSDASPISAEILPPDLL
                     VLDLIYAQTRLLRDASAAGCTVSDGEQMLLHQGAAAFTLWTGLAAPIELMQRALSEAR
                     AGGVRSAEGEPAGDDPLAAAGPATSGAA"
     gene            complement(9233..>10659)
                     /gene="aroA"
                     /locus_tag="VHM48_12540"
     CDS             complement(9233..>10659)
                     /gene="aroA"
                     /locus_tag="VHM48_12540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX2756288.1"
                     /translation="HDANAAMTTTSTDAAVIRPAARLRGELSLPGDKSVSHRALMFAT
                     LAAGESRIDGAGDGADVRSTAGVCRALGAMVERVDAAQPGRSTSATVDYLVNSPGVDG
                     LREPAANLDCGNSGTSLRLFAGILAGLPFRSVLDGDDSLRRRPVARIIEPLRAMGATL
                     LARSDDSLPPLTVIGRHPLHAVDRWTPVPSAQVKSAILLAALRAEGRTTVREAVATRD
                     HTERMLRARGIGVTTERLAPVDADGRRGAVAVSLEGGRTMTAIDERVPGDVSAAAFWL
                     VAAAIHPDADLVVRGVGVNPTRRAVIDLLRAMGADIEETAVAGRRATPASPSEEGVGE
                     PLADLRVRSSALRAIELGPDDVAAAIDEIPILCLAAAHAEGTTTIRGADELRHKESDR
                     IRGTTDGLRALGVDMTVDGDDLRIHGGRPLAGTTVDSLDDHRLAMTFAIAGLLAAGRT
                     TVLRPGSASISYPGFFDDLGRVGS"
BASE COUNT         1471 a         3911 c         3763 g         1514 t
ORIGIN      
        1 gtcgcggctc ggagcggcgg attcctgtac tgcgtgtccc tcgtcggcgt caccggcgcc
       61 cgggccgagc tccccgcaac ggtcgccgag ctcgtccgcg acgtcagcgc ggtctcgccg
      121 gtgccggtcg gtgtcggctt cggggtgagc caggcggccc acgtgcgggc gatcgcggcc
      181 accggagcgg gcggggtcat cgtcgcgagc gccctcgtcg acgcgctcgg cgaggacggg
      241 accgacgtcg ccgcgatgga gcgactcgtc cgcgatctcg cggccgccac gcgaacgagc
      301 gtcgacgccc tcatctgacc cggtcattcc tggtgcaggc cgcgatacgt gacaggtgag
      361 cgccgtctgc catcgtgacg acatgctctt cgtcgaccgt ctccgcccga tcaggcaagc
      421 cacccaggtt ctgcgtgtcg ccgcccacgt cgcgcgccgt caacggacgc tcgagcgcgc
      481 gatccgcaac atgcctgatc ccgtgccctg ggactgggcc gctccgcggc tcatgccgct
      541 cctgtccggg ccgcagttcg acgatcccga actgccaatc gtccgaatga cgtcggagct
      601 tggcccggcg atcgagttcg gactcgatct cggcggggtg ttcctgaccg tggatcgacg
      661 cgtcgccgaa cgctgggagt gctcggccga gcagctgctg gaacggggcc tcaagaacct
      721 ccgcgatcgc gccgcgaaga tcgctcccga tcaggtcgtc gcgggcgtca tgagcggtcg
      781 cacgattcgc gtcctccgcg atcggccgac gtgggcgagc tcgaccctcc tcgatctgcc
      841 aacgatccac cggctcttcg gcgcccacga ccagctcctg gccgcgccga cgacgggctg
      901 cgtcgtaagc ctgcccatcg atacgccgac ccgaaccgcg gcggacatcg tcatcgactt
      961 cgagggaccg ctgacctcgc tcttcctgga tccgttcgtc atggaggacg ggacgctcct
     1021 gtggggaggt gatgtgtccg ccgacgacct cgacgacgac tgcggcgaac catggacatc
     1081 atgatgcctt ggttatagac ttcatgatgt gtcaaccagg acgacgctga ccctggacga
     1141 cgacgtggcg gatcgcctcg cgcgtgaagc gcgtcgatcc ggccggtcgt atcgcgccgt
     1201 cgtgaacgcg gcgctgcgac ggggactgga cacggtcatc gatgcatcgg agccgttccg
     1261 cctcaactcg gctgagatgc ggcgtcgacc cgagttcgac ctcgatcaca tcgaggacct
     1321 gctcgatcga ctcgacgggc cccagcgccg atgatcgtga tcgacgcgaa tctcctgctt
     1381 tacgcgtacg acgaggcgga cccgcgccac ccggtcgtgg cgcgctggtt ccagagcctg
     1441 atgtccagct ccgacgatgt cggtctgccg ctactcgcca tcctggcgtt tatccgcctc
     1501 tcgacggacc atcgcgtctt cgagcagccg cgatcggtcg ccaccgccct cgccatcgtc
     1561 gaatcgtgcc tgtctcgccc gaacgttcgg ctgctggagc caacgggggg acactgggcg
     1621 acgctcgcgg acctggcagc ccgcggacag gcgcgtggcc ctcagttgat ggacgcgcat
     1681 ctcgccgccc tgacgatcga gcacggtgcc acgctcgcga ccgcggaccg cggatttgct
     1741 cgcttcccgg gcctgcgaac gctcgatccg accgccgcct gactcgtcgg ttgccggtac
     1801 cagacagcat ccgacggccc ctggtggcac cctgaggctc atgacgaagc ccattctcgt
     1861 gacgatcgaa cacacggccg ggaagacgtt cgcgaccgcc gccgactggc ccggctggag
     1921 ccgctcgggc aagaccgagg agcttgcgct cggggccctc gtcgcgtacg ccccgcgcta
     1981 cgccgtcatc gcggacgccg atcatcgccg gattgcggca ggcccgacga tcgaggatct
     2041 cgacgtcgtc gagcgcaacg cgggctcggc cgggacggag tacggcgtgc cgagtcggcc
     2101 gagcgaccac gacgcccgcc cgaccgaggc cgacgatgcg aagcggctgg ccgggctggt
     2161 cgaagtagcc tgggtcttct tcgatcggac cgcggccgcc gcacccgagg agctccgcaa
     2221 gggaccgcgc ggcggcggac gcaacacgtc gaaggtcatc gcccacgtga tggacgcgga
     2281 ccgggcgtac gcaaacgaga tcgggatcaa ggtccgcgag ttcgcgcctg acgatcgcgc
     2341 cgcgatcgcg gccatgcgcg ccgcgatgct cgaggtcctg cgggccgcgc gcgatggcac
     2401 gccgctcgcc ggccgtcgct ggccggcccg ctatgccgcc catcggatcg cctggcacgc
     2461 cctcgaccac gcctgggaga tcgaggaccg caccgaatag gcacgatcgc gcgtcatggc
     2521 gtggcgtctc gctcgaagat cgaaccgaat cgacccggcg acgcactcat cggcatggag
     2581 gccgtcctgg accagtcgcc gccgaccctc gcccacctcg tcgccgccgc ccgccgcggc
     2641 gatcgcgacg cgttccgcgc gctcgtcgag ccagacctgc ccgcggccct cggcacggcc
     2701 cggatcgtga cccgctccga ggcggatgcc tcggacgccg tccaggaggc gctgctctcg
     2761 gcgtggcgag gtctcgacgg cctgcgcgaa cccgaggcgt tccgggcctg gttccggcgc
     2821 cacgtcgttc gggccgcgtt gcgcgccgcc agccgccgcg ggcgcgtcgt cgagctcgat
     2881 ctcacagcga gcgcgcccga gggcgagctc gatcgggccc tcgagcaacg gaccctcggt
     2941 cgggcgttcg ctcggctgga tccgcccgac cggctgctgc tcacgctcca tcacttctgg
     3001 ggcctgccga tcgcggagac cgcggcccat ctcgacatcc cggagggaac ggtgaagtcg
     3061 cgggtccact acgcgatgga tcgactgcgc gccgcctacg cggcggagga acgacgatga
     3121 gcgacgactt cgaagacctc ctgaagcgct ggttgcgcga ccacggcgcg accgatcgtt
     3181 ccgcgatcga ggcggtggcg ggtcacgtcg ccgtgctgcc gccccggcgt cggcgccagc
     3241 tcggcccgct cgccgtcgcg gcggcggtca tcgtcgcgat cggcctggcc gccttcgcgc
     3301 tggtgccgcg gcagagctcg gtcacgagcc agccttcgag cccggtcccg ccggatccgg
     3361 cggcgttcgc cggggatgcg cgtctcgctc gctgcggcgc gtcggtcgac accgctctcg
     3421 acgtcttcga gatgggccgc gcccgcgact atcgcctgca cctgccggcg atgggcctct
     3481 cgccggagct cgatgtcgat acgccgggct tcgtcgtcgt cttccgcgac atgcagccat
     3541 ttgcggtcct gggcgcgccg ccgccggccg gccagacctg gccgccgcgc agtctggcgc
     3601 cgggccacca cgacgtctgc atcctggtcg gcgccgatcc ggcaaccgcc gagttgaaca
     3661 tctacgacga cgtcgacatc accggcctgg ccgcattcgt tgccggcacc gacccgtcga
     3721 cgtcctcggg gccggatgcc acggcgtccg ttgcggcgtc gccgtccatc gagccatcgt
     3781 tgacgcccga acctgcgccc gcctgggtcg accgtctcgc cggccagctc gaatgcacgg
     3841 gtgaggtcgc gaacatcggc gacgaggtgt ccgggacggc cggaaccgac ccgctgggcc
     3901 cgaccgcggc tgcggcactc acctccttcc tcggtcccac gaacgcgtac gcgagcctgc
     3961 cggccggtgg cttcacccag atccacgtcg acatccattg ggcgagcttc ggtcacctcg
     4021 tcggcggtcg actcaaggcc gtcatcgtcc tgaccgacat cagcgacttc gggcccggct
     4081 gggtcgtccg agggatccgg gcctgcgatg cctcggagtt cgacccggcg gtcccgctga
     4141 ccttcccggt caccatctgg accgattcct ccggtgcccg ggtctcgacc gatctgatcc
     4201 gctcgatggc cgggcctgcc cattgcggct gggacacggt gaccttcctg cagttcggcg
     4261 gggacctgta cttccgcgat ccgaagggcg ccatgaacgc gtggacgacg acgtccttcg
     4321 aaccgcgcgc cctgatcccg cgaaacgccg aagccagcgg ctaccgctcc ggcggcgcag
     4381 ccctgtggac ggttccgggc ggcgatgcgt acctggactt ctccggccat gtcgagcgat
     4441 ggccgcgatc caccgactcg accatcggct gcatgtgaac cgggtcgcgg ctgcgcggct
     4501 cgccctgaac ctggatcctg ggcgcaggcc tggggatcct gcggctcagt cgtcctcggc
     4561 cgtcggcgga accccgccga agggcgcggc ccggcgctcg cggttccggt cgcgccgctc
     4621 gaggctgcgg gcgatcacga acaggcgctt gaagatcgcg aggaggtcgg cctggggggt
     4681 cgggccgtcg ttcgccatcg agacgcggag gaggacctcg cgttcgcgca gcgggtcgcg
     4741 gatcgcccgg agcccggtcg tcgccttgac ccgcccgacc tcgcgggcca gctcggcgcg
     4801 gtcattcaac agccggacga tccggcggtc gacggcgtcg atccgtcgac gcaggcggcg
     4861 cagctccgcc ggttcgtcgg ggccggcgcc gggaccctgc cgctcagcca cccccggcct
     4921 ccccgaaccg cgcggcgatc gcctcgagcg ccaggaggta gccgcgcttg ccctgtccga
     4981 cgaacgacgc gaccgcgacg tcgtgcagga agttgaccga ccggaacgcc tcgcgggtcg
     5041 agggatccga caggtggacc tcccagaacg gccgctggac gccgagcagg gcgtcccgga
     5101 gcgagacgga ggtgtgcgtc agcccgcccg cgttgacgat cacgacgtcg aagtcgcgcc
     5161 ggtggagccg gtcgatcagc gccccctcgt ggttcgactg gaagaagtcc gcgtcgaggc
     5221 cgagctcaac agcgcgcgcg gcgatctcgg cgtggatgtc gtcgagcgtg tcgtggccgt
     5281 agatctccgg ctcgcgcgag ccgagcaggt tcaggttcgg tccctggagg acgaggacgc
     5341 tggtcatgac ggacctccgg gcggcgcgag cagtgaggat gccgcccgct cgacgacatc
     5401 tgccggcacg tcggcacgga cgacggagcc gtcggccgtc ggcaggaccc agcgcagcgc
     5461 cccgtccgcg tgcttcttgt cggcggcgag ggcgcccagg acggtcgcga gcgggtaggg
     5521 gagccgctcg gtcgccagcc cgagggccgt cagcaggcgc tcgatccgct cgccccgatc
     5581 ctccggtgtc accccgagct cccgcccgat ccggaccgct ccgcgcagcc cgtgggcgac
     5641 tgcctcgcca tggagcaggt cgccgaaccc gccggcggcc tcgaccgcgt ggccgaggga
     5701 atgccccagg ttgagtctga tccggccatc ggcggcaccc tgttccttct cgtcggcatc
     5761 gaccaccgcg accttggccc aggccgtccg ttcgacgagc tccgcgatcg ccccgccggc
     5821 gaacgccgct tcgtcgccgc gggcgaccgc ctcgccgtct cgctcgagga gggcgaagag
     5881 cagctcgtcg ccgagcgccg ccatcttgac cgcctcgccg agcgcggccc gtcgctgccg
     5941 ctccgggagg gagcgcagga acgcgatgtc gatgacgacc gcggccggcg gatggaacgc
     6001 gccgacgagg ttcttgccct ccggcaggtc gaccccggtc ttgccgccga tcgatgaatc
     6061 gatctgggcg acgagcgtcg tcgggaccgc gatgatcggc acgccgcgca gccagctcgc
     6121 ggccaggaac ccggccgcgt cgcccagcgc gccaccgccg atcgcgacga gcggctcgcc
     6181 tcgctccgcg cgcaggctgg cgagccgact cgcggcctcc tcgacgaccg ccatccgttt
     6241 cgccgactca ccctgcggca ggaggatccg ctcgaccggc cagccgtcgt cggccagcgc
     6301 ggcaccgatg gcctcgccgg ccgccgacca cgcacctggc tcggagacga ggatcgcccg
     6361 ccgggcacgg aggccgcgga gggtgtcgcc gatcagcgct gtcgcgatgc cctcaccgat
     6421 cacgagttcg ccgccgagcg aatggccgcg caggaggacg gacggctcgt cttcgcgggc
     6481 cgcgcgggcg acgagctcgt cgatccgctc gacgatggag gcgagttcgg cgacgccgtt
     6541 catccgctgg gcggcggagt agaaccggtc gcgctcgcgg gcgaggtcgc ggatcgcacc
     6601 catcggatcc cgaccggcca tcagcggccg gacgtttggc gagcggcgga gacgctgggc
     6661 cagcacctct ggccggacgt cgagccagac cgggacccgg ccgcggtaca gggcccagcg
     6721 attgcgcgga tcgatcactg cgccgccacc ggtggcgacc accgagcgga cggccggccc
     6781 gcgatccgcc ggcccgaggt cggcgacggc ctgccgctcg agcgcccgga aggcggcctc
     6841 gccgcgttcg gcgaagatct ccgggatcgt cttcccgacg gatttctcga tcacgtcgtc
     6901 gaggtcgacg aacgacgcgc cgtggcgatg ggcgagccgc cgcccgacgg cactcttgcc
     6961 gctgccaggg agcccgacga ggacgacgtc catgtctcga tccggtctag tcgtcgccgc
     7021 cggagccggc ctcgtcgccg ccggccgcga tcggatcgac ggcaccgcgc cgaccgccgg
     7081 cgacgggggc gccgatcggc ccggagatcc gcgcgacata tcgatcgagc gcgtcgcggg
     7141 tgtcggcgat cgtgtcgcca ccgaacttgt cgagcatcgc gtcggccaga gtcagcatga
     7201 ccatcgcctc ggcgacgacg ccagctgcgg gcacgacgct gatgtcggac cgctcgtagt
     7261 gggccttgtc gaccgcttcg ccgctgacga ggtcggccga cggcaggggc cgggccagcg
     7321 tcgagatcgg cttgacggcg ccgcgcacga cgagcggctc gccgttcgtg atcccgcccg
     7381 tcaatccgcc ggcgttgttc gagcggtgga tccagcggcc gccctcgccg cgccccgcga
     7441 tcacatcgtg gaccgccgac ccgaaccgct tcgtctgctc gaagcccagc ccgaactcga
     7501 cgcccttgac gatgttgatg ctcatcaccg ccgccgccaa tgccgcatcg aggcggcgat
     7561 cccagtggac atacgatccg agcccgatcg gcaggccgtg ggcgacaacc tcgaacaccc
     7621 cgccgaccgt gtcgccgttc gagcgtgcct cgtcgatccg ggcaatcatc gccgcctccg
     7681 cggcaggatc cggacagcgc agcggcgacg cgtccgcctc gtcgcgcgta cgggtcgccc
     7741 gcgccgggtc gaccgccacc ccgccgacct ccgccgtgaa tgaccagacc tcgacaccga
     7801 gcgcgccgag gatcgcccgg gcgacaccgc cggccgcaac ccgtgccgcc gtctcgcgcg
     7861 ctgacgcccg ctcgaggacg ttccggacat cgttgaagcc atacttgagc gccccggcca
     7921 ggtccgcgtg tcccggtcga acgcgggtga ccggcgtcgc ccgcttgttg ccgtcagcgg
     7981 cggctgccgc cagctcggcc gactcgtcgt cggacaggcc ctcgacctgc atgacccgcg
     8041 tccagttgtc ccagtcgcga ttggcgacga ggagcaggat cggcgatccg agcgtcatcc
     8101 cgtggcggac gccgccggcg atgtcggccc gatcctgttc gatcgcctgg cgcgccccgc
     8161 gcccgtagcc gcgctggcga cgcgcgagat cgatcgcgag gtcatcgccg ccgagcggca
     8221 gccccgccgg gacgccctcg acggtcaccg ccagcgtcct gccgtgcgat tctccggcgg
     8281 tcagccagcg aagacgtccc atcaggtggt ggaccggccg gtcaggccgc gcccgaggtc
     8341 gccggcccgg ctgccgcgag tgggtcgtcg ccggcgggtt cgccttcggc cgatcgaacg
     8401 ccgccggcgc gcgcctccga gagagcccgc tgcatgagct cgatgggagc cgcgagcccc
     8461 gtccacaggg tgaacgccgc ggcgccctgg tggagcagca tctgctcccc gtctgacacc
     8521 gtgcaaccgg cggccgaagc atcgcgaagc agtcgcgtct gggcgtagat caggtcgagg
     8581 acgagcaggt cgggcgggag gatctcggca gagatggggg aggcgtcgct cgtcagcccg
     8641 atcgaggtcg cgttgacgag gaccttggac ttggcgagct cggactcgat gatcgactcg
     8701 tgccagggca tggcccgcag ctccatgtgg gcggcgctcc gcccgaagtg cttgaccagc
     8761 gcctccgccc ggtggagatg gcggttgaac acgatgatcc tctggaagcc ctcgcggatc
     8821 aggccgaaga cgacggcccg cgacccgccg cccgcgccga ggacgatcgc gtgacgtggc
     8881 attttctggc gcccgacgag cttgtcgagg gcgatcttga agcccgggac gtcggtgttg
     8941 tggccgacga ggcgcttgcc ctcgcgggtg atcgtgttga ccgcgccggt tgcctgggca
     9001 tcctcggtga ggcggtcgac gagcggcacg acccgctcct tgtgggggat cgtgacgttc
     9061 gccccgagaa actcgtcgcc gcggatctcg ccgatcgcgt cgggcagggc gatcggggcg
     9121 cggtcccaga gctcatacgc ggcatcgatg ccctggtcgt cgaaggcggc ctgatgcatg
     9181 gcgcccgaca gcgagtgggc aacgggatga ccgatcagga cgacgcgctt ggtcatgagc
     9241 caacccttcc gagatcgtcg aagaagccgg gataggagat cgaggcgctg ccgggccgga
     9301 ggacggtcgt ccgaccggcg gccaggagac cggcgatcgc gaacgtcatc gccaggcgat
     9361 ggtcgtcgag gctgtcgacg gtcgtccccg cgaggggccg cccgccgtgg atacgaagat
     9421 cgtcgccgtc gacggtcatg tcgacgccga gggcccgcag tccgtcggtc gtgccgcgga
     9481 tccgatcgga ctccttgtgg cggagctcgt cggcaccgcg gatcgtggtg gtcccctcgg
     9541 catgggccgc ggcgaggcag aggatcggga tctcgtcgat cgcggccgcg acgtcgtcgg
     9601 gcccgagctc gatggcccgc agcgcggacg agcggacccg gaggtccgcc agcggctcgc
     9661 cgacgccttc ctcggagggc gaggcgggtg tggcgcgtcg gccagcgacc gcggtctcct
     9721 cgatgtcggc gcccatcgcc cgcagcaggt cgatgaccgc acgtcgcgtc gggttgacgc
     9781 ccacgccgcg gacgacgaga tcggcgtcgg ggtggatcgc tgccgcgacg agccagaagg
     9841 cggccgccga cacgtcgccc gggacgcgct cgtcgatcgc cgtcatcgtc cgccctcctt
     9901 cgagactgac cgccaccgcc ccgcgacgcc cgtcggcgtc gaccggcgcg agccgctccg
     9961 tcgtgacgcc gatgccacgc gcccgcagca tccgctcggt gtgatcgcgc gtcgcgacgg
    10021 cttcgcggac ggtcgtccga ccctccgccc ggagtgccgc caggaggatc gccgacttga
    10081 cctgcgcgct cgggaccggg gtccaccgat cgacggcgtg cagaggatgg cgaccgatga
    10141 cggtgagtgg gggaagggag tcgtcggatc gggcgaggag cgtcgcgccc atcgcgcgaa
    10201 gtggctcgat gatacgggcg accggtcgcc gtcgcaacga atcgtccccg tcgaggacgc
    10261 tccggaacgg aagcccggcc aggatcccgg cgaagaggcg caggctcgtc ccggagttgc
    10321 cgcagtcgag gttggccgcc ggctcgcgca gcccgtccac gcccggcgaa ttgacgaggt
    10381 aatcgaccgt cgcggacgtg gaccgaccgg gctgcgccgc gtcgacccgc tcgaccattg
    10441 ccccgagcgc ccgacagacg cccgccgtcg agcggacgtc ggcgccgtcg ccggcaccgt
    10501 cgatccggct ctcgccggcc gcgagcgtcg cgaacatcaa ggcccggtgg ctgaccgact
    10561 tgtcgccggg cagcgacagc tcgccgcgca gccgggcggc cggccggatg acggcagcgt
    10621 cggtcgacgt cgtggtcatc gccgcgttcg catcgtgga
//
LOCUS       DATAIW010000418         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_58715, whole genome shotgun sequence.
ACCESSION   DATAIW010000418 DATAIW010000000
VERSION     DATAIW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58715"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2759
                     /locus_tag="VHM48_12545"
     CDS             <1..2759
                     /locus_tag="VHM48_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2756289.1"
                     /translation="GFALEGDGPGEDVSLIELLREPARRAPDSLAGQLRYVREHWGWL
                     LGTALDELLGRIDIAIGVLTEEEHGLHVRFGGFGGGGGGGGEAPSFAGAEVEAERFSE
                     DLDWMPRLVLLARSTYVWLDQLSKRYGRDIRTLDAVPDEELASIARRGVTGLWLIGLW
                     ERSSASERIKRWRGNADAVASAYSLDDYRIADDLGGEDALADLRTRAWRHGIRMASDM
                     VPNHMGIDSRWVIDHPERFIGLDEPPYPAYRFEGKNLASDPRIEIRIEDHYWDDSDAA
                     VVFERRDTATGERRYLYHGNDGTSFPWNDTAQLDFLRADVRETVIRTILDVARRFPVI
                     RFDAAMVLAKKHVERLWYPEPGAGGAIPSRAERAISKARFDELMPEEFWREVVDRVAA
                     EAPDTLLLAEAFWLLEGYFVRTLGMHRVYNSAFMHMIRDEDNAGYRKVILDTIEFDPA
                     ILGRYVNFLTNPDEETAIEQFGTGDKYFGAATMLATLPGLPMIGHGQIEGFTEKYGME
                     YRRARLDEVPNAGLIGYFEAQIVPLLRERRRFAGSADFRLYDVVGDDGSTVEDVFAYS
                     NGRAEERSLIVYHNRFADSAGWIRDSVSFAVKHDDGTKTTRRDTLGDALGLVGPADGW
                     LRFRDRRAGLESLRSVGEIRDRGLRVELGAYECLVLDDLREVVSTAETPWAGLADELH
                     GRAIHSLDEALADHRLRPVHATVGRLLGAMTPDDVAGRRDELLAAAGLPPAPTRHPEA
                     ASRATAGKPVQPVGIDPAARAAVRLAPLDRARFDDLRVGVALRAAGFDQEAIERTRVA
                     LGLATPSAVPAAAALVTAWLADPAVRAFLQVHEWDGAEWLVRERWVALVRLADQLDRA
                     LGAKRTSPAIARLLPAAEAALDRVDAIPHVVSPSRARSAAVVAASGPRPDGPPSARDR
                     RRRS"
     gene            complement(3043..>4236)
                     /locus_tag="VHM48_12550"
     CDS             complement(3043..>4236)
                     /locus_tag="VHM48_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX2756290.1"
                     /translation="AVLPRLRAARTIAADALDAFERRLRDEILPEAAGEGRLGPERFA
                     ARMTHTMHDPAMTPDRIREQAEREFAAVRAEMIRIAREIAPDWLGDNPIPDDDGDVVR
                     AVLDSVAIDHPKADELLDFCRAELARIETFCGEVDLVGLSKEPLEIGWTPLFLRAFGG
                     AMLSSPGPLERGQKAIFSITPIPADWTADQAESSLREDNARMLRLLTIHEAVPGHYLQ
                     GVYANRCPSIARALFGSGVFAEGWAVYVTQVMMDAGYGADDPALMLTHWKYYLRCVVN
                     ALIDVGIHTAGMTEDEAVSLMIDGAFQEEAEARAKYNRARLSSTQLSTYFVGSFLFWD
                     LEHEVRRRAAAAAGGVGGADAVPTPRVVGGYGKTPGFVYRDHLEECMNHGSPPMPLLR
                     RLVLG"
BASE COUNT          595 a         1536 c         1502 g          603 t
ORIGIN      
        1 cgggattcgc cctcgagggc gatggtcccg gcgaggacgt gtcgctgatc gagctgctgc
       61 gggagcccgc ccgccgcgcc ccggattcgc tcgccggcca gctgcgctac gtccgcgagc
      121 actggggctg gctcctcggc acggccctcg acgagctgct cggccgaatc gacatcgcga
      181 tcggcgtcct gaccgaggaa gagcacggcc tccacgtccg gttcggcggg ttcggcggcg
      241 gaggcggcgg tggcggcgaa gcgccatcgt tcgcgggtgc cgaggtcgag gccgagcgct
      301 tctccgagga cctcgactgg atgccgcggc tcgtgctcct cgcccggagc acctatgtct
      361 ggctcgacca gctctcgaag cgctacggcc gcgatatccg gacgctggac gcggttcccg
      421 atgaggagct cgcctcgatc gcccggcgcg gggtcacggg cctgtggctg atcggtctct
      481 gggagcgctc gagcgcatcc gagcggatca agcgctggcg cggcaacgcc gacgccgtgg
      541 cgtccgcgta ttcgctcgac gactaccgga tcgccgacga cctgggcggc gaggatgccc
      601 tcgccgacct ccggacccgc gcctggcgcc acggcatccg gatggcgagc gacatggtcc
      661 cgaaccacat ggggatcgac tcgcgctggg tcatcgacca cccggagcgc ttcatcgggc
      721 tcgacgagcc gccctacccg gcctatcggt tcgagggcaa gaatctcgcc agcgatcccc
      781 ggatcgagat ccggatcgag gaccactact gggacgacag cgatgcggcg gtcgtcttcg
      841 agcggcgcga caccgcgacc ggcgagcggc gctacctcta ccacggcaac gacggcacga
      901 gcttcccatg gaacgacacc gcccagctcg acttcctcag ggccgacgtc cgcgagaccg
      961 tgatccggac gatcctcgac gtcgcccggc gcttcccggt catccgcttt gacgcggcga
     1021 tggtcctcgc caagaagcac gtcgagcggc tctggtaccc ggagccggga gccggcggcg
     1081 cgatcccctc gcgggccgag cgcgccatct cgaaggcccg cttcgacgaa ctgatgcccg
     1141 aggagttctg gcgcgaggtc gtcgaccggg tcgcggccga ggcaccggac acgctcctcc
     1201 tggccgaggc gttctggctg ctcgagggct acttcgtccg gaccctcggg atgcatcgcg
     1261 tctacaacag cgcgttcatg cacatgatcc gggacgagga caacgccggc taccggaagg
     1321 tgatcctcga cacgatcgag ttcgaccccg ccatcctcgg gcgctacgtc aacttcctga
     1381 cgaacccgga cgaggagacc gcgatcgagc agttcgggac cggcgacaag tatttcggtg
     1441 cggcgacgat gctcgcgacg ttgccgggcc tgccgatgat cggccacggc cagatcgagg
     1501 gattcaccga aaagtacggg atggaatacc ggcgggcccg gctcgacgag gtccccaatg
     1561 ccggattgat cggctatttc gaggcccaga tcgtgccgct gctgcgggag cgacggcgat
     1621 tcgccgggtc ggccgacttc cggctgtacg acgtcgtcgg ggacgatggg tcgaccgtcg
     1681 aggacgtgtt cgcctactcg aacggacgcg ccgaggagcg gtccctgatc gtctaccaca
     1741 accggttcgc cgacagcgcc gggtggatcc gcgatagcgt ttccttcgcg gtcaagcacg
     1801 acgacgggac gaagacgacg cgacgggaca ccctgggcga cgcgctcggc ctcgtcggtc
     1861 cggccgacgg ctggctccgg ttccgcgatc gacgggccgg tctcgagtcc ctccgctcgg
     1921 tcggcgagat ccgcgatcgc gggctccgcg tcgagctcgg cgcctacgaa tgtctcgtcc
     1981 tcgacgacct ccgcgaggtc gtctcgaccg ccgagacccc gtgggccggg ctcgcggacg
     2041 agctccacgg ccgggccatc cactcgctcg acgaggccct cgccgaccat cgccttcggc
     2101 cggtccacgc cacggtcggg cgcttgctcg gcgcgatgac gcccgacgac gtcgccggcc
     2161 ggcgcgacga gctcctcgcc gcggccgggc tgccgccggc gccgacccgc catccggagg
     2221 cggcgtcccg ggcgaccgcg ggcaagccgg ttcaacccgt cggcatcgac cctgcggcac
     2281 gcgccgccgt ccggctggcg ccgctcgatc gcgcccggtt cgacgacctt cgggtcggcg
     2341 tggccctgcg tgccgccggc ttcgatcagg aggcgatcga acggacgcgt gtcgcgctcg
     2401 gcctggcgac gccgagcgcc gtgcccgccg ccgccgctct cgtgaccgcc tggctggccg
     2461 acccggcggt ccgggcgttc ctccaggtgc acgaatggga tggcgccgaa tggctcgtcc
     2521 gcgagcgctg ggtcgcgctc gtgcggcttg ccgatcagct cgatcgggcg ttgggcgcga
     2581 agcgcacatc gccggcgatc gcgcgcctcc tgccggccgc cgaagcggct ctcgaccggg
     2641 tcgacgcgat cccgcatgtc gtgtcgcctt cgcgagcccg ttccgcggcg gtggtggcgg
     2701 cttcgggccc acgaccggac ggaccgccct cggcgcgcga ccgccgccgg cggagctgac
     2761 cgccgacatc ggctggccag cagtccaccc gtccgccggg gcggacccga atgttctcgt
     2821 ggtggccgtt ccggaccgcg gctgggacgg caggccgcga aatcctcgtg gacccctcga
     2881 atacccaggg attgacgcgt cgttgcggtc attctcgcct cgacgagcct accgccaggg
     2941 atcccgccga cgtaccgtcc gtggggtgag cattcggcag cgcgccggcg cggcgccggc
     3001 gtggcccctg cgcgccggcg cggccctggc gccgcccgga ccctagccga gcacgaggcg
     3061 ccggagcagc ggcatcggcg gcgacccgtg gttcatgcac tcctcgaggt ggtcgcggta
     3121 gacgaacccg ggcgtcttgc catagccgcc gacgacccgc ggcgtcggga cggcgtcggc
     3181 gccgcccacc ccaccggccg cggccgccgc acgccgccgg acctcgtgct cgagatccca
     3241 gaacaggaac gagccgacga aataggtcga gagctgggtc gacgacaggc gggcccggtt
     3301 gtacttggcc cgggcctcgg cctcctcctg gaaggcgcca tcgatcatca gcgacaccgc
     3361 ctcgtcctcg gtcatgcccg cggtgtggat gccgacgtcg atgagagcgt tgacgacgca
     3421 gcgcaggtag tacttccaat gggtcagcat cagggccggg tcgtcggcgc cgtagccggc
     3481 atccatcatc acctgggtca cgtacaccgc ccagccctcg gcgaagacgc cgctgccgaa
     3541 gagggcccgg gcgatcgacg ggcagcggtt ggcgtagacg ccctgaaggt aatggccggg
     3601 caccgcctcg tggatcgtca gcaggcggag catccgggcg ttgtcctcgc gcagggacga
     3661 ctcggcctga tcggccgtcc agtcagcggg gatcggggtg atcgagaaga tcgccttctg
     3721 gccgcgctcc aacggcccgg gcgagctcag catcgccccg ccgaacgccc gcaggaacag
     3781 gggggtccag ccgatctcga ggggctcctt ggaaagcccg acgaggtcga cctcgccgca
     3841 gaacgtctcg atccgggcga gctccgcccg gcagaagtcg agcagctcgt ccgccttcgg
     3901 gtggtcgatc gcgaccgagt cgaggacggc ccggacgaca tcgccgtcgt cgtcggggat
     3961 cgggttgtcg ccgagccagt cgggcgcgat ctcgcgggcg atgcggatca tctcggcccg
     4021 gaccgcggcg aactcgcgct ccgcctgctc gcggatccgg tctggcgtca tcgccgggtc
     4081 gtgcatcgta tgggtcatcc tggcggcgaa acgctccggc ccgagccgac cttccccggc
     4141 ggcctccggc aggatctcgt cccgcaaccg gcgctcgaac gcgtcgaggg cgtcggcggc
     4201 gatcgtccgc gcggcccgta gccgcggcag gacggc
//
LOCUS       DATAIW010000419        18914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_131616, whole genome shotgun sequence.
ACCESSION   DATAIW010000419 DATAIW010000000
VERSION     DATAIW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18914
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131616"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2297
                     /locus_tag="VHM48_12555"
     CDS             189..2297
                     /locus_tag="VHM48_12555"
                     /inference="COORDINATES: protein motif:HMM:NF040318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX2756291.1"
                     /translation="MSADRRRESIDDLDALLAALPREIVDAVHALPEKEALIEVVLDL
                     GRRPEARFPDSEVTLLDREISEADIAHVVEHIGTFGDDNRAGIERTLHRISAIRNRSG
                     KIVGLTCRIGRAVYGTIDIINDFVDSGKSILIMGRPGIGKTTMLREAARVLADDLGKR
                     VVVVDTSNEIAGDGDIPHPAIGKARRMQVRTPSLQHEVMIEAVENHMPQVIVIDEIGT
                     ELEAQAARTIAERGVQLIGTAHGNNLDNLMLNPTLSDLIGGIQSVTLGDEEARRRRTQ
                     KSVLERKAPPTFDVIIEIQDRERVTVHSDVAETVDSMLRGDSVAPELRWRDEEGVHRS
                     QARPRPSPREQIGAERFAGLVGSGQPWRMEPGWRGDGGYRGGGYRDAGGGGGTAGGAG
                     GAGFRSGYRPGASGGWRQTRGGSGRETAPRGPQSEGGAADVIPGERFLTPPAGGRVGG
                     RLDDPPFVSGEIADRGPLERGAPVVPDTRAREARELDRQKAWRDQAGRALTALKADEG
                     TAPVNADELDDDEDALLPLGIEEGDHGEAVLVPGGSPLPTLRVLPQGISRKRLEAAIR
                     DLQLPVLIARDVDDADVVMTLRNEYKQKTPLLREAEERALPIYVLKSNTVPQMQSSLT
                     SIFSLEIDPREAAMRETEEAIGMVLHGSEPVELSPQNAYIRRLQHQMAERANLVSRSR
                     GREPYRRVRLYPDAARPAWR"
     gene            2305..2706
                     /locus_tag="VHM48_12560"
     CDS             2305..2706
                     /locus_tag="VHM48_12560"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2756292.1"
                     /translation="MSSDAAPPEGATRPRLGRLHPHNVVIDVADHDAVVPFWEAALGW
                     TVQRINDQFVGLRAPADEAIGFDILFQKVPEPKTSKNRAHIDFDAADMEAEVERLLGL
                     GGTKLAEHNLGAFRWTVVADPEGNEFCVTIR"
     gene            2747..3484
                     /locus_tag="VHM48_12565"
     CDS             2747..3484
                     /locus_tag="VHM48_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756293.1"
                     /translation="MTDVPAEWRRGLFRLANALIVYGVIGILAAAIGIAALIGASGRI
                     GSLGERIVSEAVSLDAIIGSSADVLDGAATTVGTLGPTVERTSATVSAAAGALRDIEP
                     RLRDVETQANAFEIFGQRPVASLGQLFGQIATDISGLDEQLDGIATEMGRTKAALTAN
                     AESLGALADLIRAYKDRLKPEAIDAGIDEARRLLLFTLTLFIGWTAVPAIGSLIVGRW
                     MRRLVEPERVPPSESPGPAPMIDSAGS"
     gene            complement(3504..4940)
                     /locus_tag="VHM48_12570"
     CDS             complement(3504..4940)
                     /locus_tag="VHM48_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2756294.1"
                     /translation="MSGGPEAVVVGAGPNGLAAAITLARAGRSVHVYEAAATIGGGTR
                     TEELTLPGFRHDVCSTILPLTLASPFFRSIDLAARGIEIVQPDAPVGQALDGGRAAIV
                     ERSVAETAAALGRDGPAWRRLFGPLVADAEKLGAELLRPVIHVPRHPLALGRFGLPAL
                     LSATGLARITFRDEPARALLAGLAAHSMVDLHRPLSASFGLVLGLYAHAVGWPMIRGG
                     SAAVADGLAAELRSLGGTIETGRLITSLAELPPARAVILDTTPRAALEITGDRLPVRT
                     RRLYERFRYGPGVFKVDWALGGPIPWRAEALRRAATIHLGGTLGEVAAAERTVALGGH
                     PDRPFVLCVQYSPWDDSRAPAGKTTAWGYCHVPAGSTVDMTDRIEGQVERFAPGFRDR
                     ILARATRGPAELEAENPNYVGGDINAGIEDIRQLIFRPGPLLDPYWTGAPGIYLCSSS
                     TPPGGGVHGMSGYLAARSALHREFGGRG"
     gene            complement(4963..5685)
                     /locus_tag="VHM48_12575"
     CDS             complement(4963..5685)
                     /locus_tag="VHM48_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756295.1"
                     /translation="MEPKDTGAWAHPVSSLATTAVGPDDAVTGRRVAGPVHGFGQMWQ
                     KTFRVPLDGGASPRDVIATWKERFPTCWPKSVKFYAPLAGIAPGEVALLEIALLPGAP
                     VRLSTGVMVIYADDESFTFMTPEGHTLSAWITFSAFDESGVTLAQVQVLERPSDPLDD
                     LAYNLGGSRMQTRFWEETLIALARHLGTSDPSVETTVVCVDRKRQWRYVRNLRHSATI
                     HSMMRTATAPSRWVASRVRGGG"
     gene            complement(5700..6059)
                     /locus_tag="VHM48_12580"
     CDS             complement(5700..6059)
                     /locus_tag="VHM48_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2756296.1"
                     /translation="MADPGTRVDVRSTDDPTASVVAIFGDVTGGSEPALMEAYSKAVD
                     GRARTIVLDFAGLEYMNSGGIGLLVTMLVRSKRQGQRLLAVGLSDHYREIFSLIRLDE
                     AIGIHEDANAALAAGRA"
     gene            complement(6052..6258)
                     /locus_tag="VHM48_12585"
     CDS             complement(6052..6258)
                     /locus_tag="VHM48_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756297.1"
                     /translation="MTALGVWVIAEGEFMNSMLEAVPAELSEHYREIFRITRLSDYVT
                     LEDDQAVEPVPVHAGVEEGAQDHG"
     gene            complement(6305..7537)
                     /locus_tag="VHM48_12590"
     CDS             complement(6305..7537)
                     /locus_tag="VHM48_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756298.1"
                     /translation="MTTGRAERRPGKLSVLMIVTSIGASIAISVAMDVVLLRYKVGAT
                     PDVFVGVLFNGTCILQSVVGAIIEWRRPGHTIGRLLMLSGPLYALLAVVWLTADTLES
                     FVDPQLYRVVTWGGTLLSYPGMALIAGWVPLLFPTGTLPGPRWRIPVGLLVVLSSIGL
                     VASAVPVWTISGRVRGSPIVFDAWPPYLQPFVDAIPLELMALIALAIAGLITRYRRGD
                     RIERLQIRWFGAAVAVLGVGFGGVLVEMAVRTGDGPLVSALIPYAGILAMPIAIGIAV
                     TRYRLYEIDRLISRTIGWAVVTGILGAVFAATIVGLQTILADVTQAQTLSVAASTLVA
                     FVLFQPLRRRVQSVVDHRFNRARYDAERTAAAFAERQRDQVDIAGLKVDIAGTIDSAL
                     SPRTVGIWIRPSGRGGMP"
     gene            complement(7599..8804)
                     /locus_tag="VHM48_12595"
     CDS             complement(7599..8804)
                     /locus_tag="VHM48_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756299.1"
                     /translation="MMRWSILAPWLITIALFLGLVATAAVAGQHPGIGGGGAELVAFF
                     GLIGIWGVANATVGALIASRRPENRIGRILQAGGPLVIGVFLGFLISAIRAVTAGPGD
                     LLGALAGWWAAVTIFPAILVAWPLVAILFPDGRLPGTRWRGPVVFIAIGEVAISGIAA
                     ITVGPVAPGLPDNPLGVLSLTPGIMTLLGLGGTALLVLSLVLAVTAIGVRWHRGDRLL
                     RAQLKWLLGALGVGAVLFPLGFGGEVVNPLDFLGVASATLMPIGIGIAILRYRLYEID
                     RIISRTLTYGLLTLVLVGVYAAGVALLQTVLAPFTSGGGPVAVAASTLAAVVLFQPLR
                     RRIHAAMDRRFNRSRYDAQLTVHGFAGQVRDEVDLERLRDHLRAVIDSSLAPTSVSVW
                     LRSSDRTAR"
     gene            complement(8851..9102)
                     /locus_tag="VHM48_12600"
     CDS             complement(8851..9102)
                     /locus_tag="VHM48_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756300.1"
                     /translation="MPKAARPDGLIQLGRLGQRHDAQVAIENSDAVAVLADCGRRLAR
                     LSQELRAGSQAWLLVAVEATFAAAWASFAFSERPSVGRA"
     gene            complement(9608..10348)
                     /locus_tag="VHM48_12605"
     CDS             complement(9608..10348)
                     /locus_tag="VHM48_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756301.1"
                     /translation="MTQTTPRPGRDQDAWARPVARLTTTATGAGTDTVTGKRVAGPIQ
                     GFGQMWQKTFRVRMDGVDVAPDTVISHWKAEFPTFWPKGATFYAPLSGISPGEVALLE
                     VPPIPGSPIKMSTGVLVIYADHESFTFMTPEGHALSAWITFSATRDDQGTTAQVQALE
                     RTSDPLIEISYLLGANRMNDRFWEQTLENLARSIGVANPTVTSTKVCVDRRRQWRQVG
                     NVRNSPALSMALGTVMAPVRWLRRRRSA"
     gene            complement(10375..10728)
                     /locus_tag="VHM48_12610"
     CDS             complement(10375..10728)
                     /locus_tag="VHM48_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2756302.1"
                     /translation="MTDGIRVEVRSTDVAEASLLHIEGEITSASEQPLMDAYARAVDG
                     GARTVILDFGALDYMNSGGIGLLVTLLVRAQRQGRRLMAVGLNDHYRQIFSLTRLDEA
                     IGIHDSTEAAVAAVA"
     gene            complement(10725..11084)
                     /locus_tag="VHM48_12615"
     CDS             complement(10725..11084)
                     /locus_tag="VHM48_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2756303.1"
                     /translation="MTSPLSATVRPAADGARIVLSGRIDRDADAAMADAYGEARATGS
                     GPITLDFAATDYINSTGIALIVRLLAEARRDNRAVRAMGLSEHYREIFRITRLSDFMT
                     LDDDSTVDEPGHNGGAK"
     gene            complement(11081..12721)
                     /locus_tag="VHM48_12620"
     CDS             complement(11081..12721)
                     /locus_tag="VHM48_12620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2756304.1"
                     /translation="MTALSRPFRRLLGRRAPAPAPITGMAPAVPAVDERPGVAIDIPE
                     TDPLFAFLQSAPGPVDLARLELASPVLEDLRGAGVALVVPLVTQGELIGTLNLGPRLS
                     EQDYSSDDRRLLGTLAAQAAPAIRVAQLVREQAAEAAERERYEQELRVAQLIQQQFLP
                     RELPNLPEWQVAAYYGPARVVGGDFYDFIDLGDGRIGLVVGDVTDKGVPAALVMARTH
                     SILRAEAPRLVAPGAVLARANELLVAEMPEKMFVTCLYMVLEPATGHVVYANAGHNLP
                     YVRTAGGVVEFRATGMPLGLLPGLVYEEKEADIAPGDSVLLYSDGIIEAHDPGGDMYG
                     FPRLKADMASELAGSDLLDELLEHLHEFTGRGWEQEDDITMVALRRSVGGGSVTADEA
                     ISEPPSTTVDVIAEFELPGAFGNERLAMERVATAVESIGLPPDRLERLKTAVSETAMN
                     AIEYGSAGDPEVPVRIRIERQPTELVVRIADAGLGGTVPDLADAEVPDLDAKLAGLQK
                     PRGWGLFLIQQMVDRVEVIREGERQTVVLTLELGGSGE"
     gene            complement(12832..14004)
                     /locus_tag="VHM48_12625"
     CDS             complement(12832..14004)
                     /locus_tag="VHM48_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756305.1"
                     /translation="MKRAQLTWGIAGLDIVVILATRLIDPAGDISGVVIFTIGVASFA
                     AVGALLDTRVPGNRIGLLLLAAGTLMSAAMATGTYASLGALQEPPWPSSGAALIAGST
                     LFVYPFMIALIGVPLVFPDGHLPSGRFRWVVRLTIANVVAWTLGAFLRLPPSDGSQAG
                     ASDQAGPDPLLGAIQTFFFVTTIVCFGAGAIAVWLRFHRGDRVQRQQIKWLAAIVGLG
                     AVVLPVSFIPDSSSDLSPVINTVTILTLFALPVVIGIAILRYRLYEIDRIVSRTIGWA
                     VVTGVLGAVFVVGLVGLQAALSGVTQGGTLAVAASTLVAFVLFQPVRRRVQAIVDRRF
                     DRSRYDAERTVGGFAERLRNEVDLGTLRTALIRTADDAVRPVNAAVWLRVGSETER"
     gene            complement(14001..14516)
                     /locus_tag="VHM48_12630"
     CDS             complement(14001..14516)
                     /locus_tag="VHM48_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756306.1"
                     /translation="MTSTISPGDSRFTGPTAIALAATALVLESRAILAANIGGGAPIM
                     ASLVVRFRRATAEERRKVKLLTLAGVVLIALQGVLSQVIGGNTLAVAGSTLVVAALFQ
                     PMRRRVKGALDRRFDRARYDAQRTVDAFAEEVRNEVDLARLRTALLATAGDAVRPVSP
                     AVWLRTGPADR"
     gene            complement(14513..15751)
                     /locus_tag="VHM48_12635"
     CDS             complement(14513..15751)
                     /locus_tag="VHM48_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756307.1"
                     /translation="MTVTRPRIVPTSGAPVATPYPLGRPARVLLGVLLIAGAIVATPS
                     GFGLLFFLPNAAVGTLLAIRRPRNSVGWILLGLGWGFAIVTMSVDLTPAQFAAGNLDP
                     ITVGLAVIGAASGPALFLLFAVLAIVFPSGRLPGGPWRRITRLAIVGGLLTVGAGTVM
                     PMSSVSIRGSAASVPVRNPAAVLPDLAIWQVITPDTAILPIVALMIGATISLLVRFRR
                     ASGIERQQLRWITTALAFVALAVVSGFVVTSLAPATTDSALAWLGAIVAFPCVPLAIG
                     IAVFRYRLYEIDRIISRTISWAIVTAVLVGVFAASVFALQAVLDPFTHENTFAVAGST
                     LTAAALFQRVRRRIQHVVDQRFDRASYNAQRTVDTFSDALRDQVDLGTIILALSVACS
                     RTMRPTSSLVWMRRREARPR"
     gene            15827..18712
                     /locus_tag="VHM48_12640"
     CDS             15827..18712
                     /locus_tag="VHM48_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2756308.1"
                     /translation="MSRPPLPSARSRSGLEVRVLGPLDARVDGASVVVDTRKALAILT
                     LLAVERRPYARDELAAMFWPESDEESARGALRRTLSVLRAGLGERWLRVDRTVIALDM
                     AGVQLDLATLEGAQGSSDRTLLSHAAEAARGPFLAGFSLRDSPDFDDWRATQGAAVER
                     MVATVLDRLSAAAELAGDLVGAVAAAARRVDLDALDESAQRRLMTLLVRSGDRAGAIR
                     QYRTSVAILERELGVSPLAATTDLYESIRDSRLDPVLAQVAAAPVPVARRLPLVGRDA
                     ELAALLRTHADSTPSGRLVLVTGEAGIGKTRLGEALADIVEGNGGTTLLARAWSAETA
                     IPYGPIVELLRAGIGRPDASDRLARLPPRALVDLERLVTLPPTLAVRAPVGSTAADPG
                     GPQVVATEDRSAARVRMLDAVAEALSALVGGPIPGVIIVEDVQWADDATREALTWLAR
                     RLNDRSLILVLSWRPEDLDDRGLAFAARLEEVAAIDPLRLQRLDRSAVTAMVEAVPTA
                     NRAAIEVDVVVEESEGIPLYVVEALAAASGRSSDGPHHGVRALLRERLATVGEMASQV
                     LAAAAVIGRSFDLATVRAASGRTEDETVAALEELVRRGIVRELGDGGSLAFDFAHAKL
                     RDTAYEGTSLARRRLLHRRTAELLRSMPGAAQDRGRLAQIARHERSAGRDADAAEAFR
                     EAGVRSRSVYANREALEHFETALALGHPDIVGLQLTIGELRTMVGDYAGASAMLEAAA
                     AVASERDLPAIEMSLGLIHVRRGDLDSAASHLDAAIESLEDRDGAERAMLARILVERG
                     LVALRARDLERARGCAGDALVMAEEAKDPAAVGAAHRLLGLVARERGDLVVARAELER
                     SVALASGDPDGATAIAAGNSLALVEAAAGDRAAAIDRLERALGACQRTGLRHLEAAVE
                     NNLADQLHAVGRVEDAMDHLKRAVVIFADVGGTPGELEPELWKLVEW"
     gene            complement(18735..>18914)
                     /locus_tag="VHM48_12645"
     CDS             complement(18735..>18914)
                     /locus_tag="VHM48_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756309.1"
                     /translation="PQGVFEVATLTGTNEGPWGGAPASGLPVSLQVLILFPWDPVTQR
                     FLGERIWFDRGTLGG"
BASE COUNT         3007 a         6589 c         6695 g         2623 t
ORIGIN      
        1 cgtcacgccg cgagcacggg gtgatcgccc cggtccggac ctacgagtcg ccgatcgatc
       61 ggcggctcga agaggccggc ttcggtacga tcgccacggt gaccctgttg atgaaggaca
      121 cgctcgtgcg tgtcgccgag ccggccctcg tgccggccgg ggtccgttga cccccaagga
      181 gggatcggtt gtccgctgac cggcgccgcg aatcgatcga cgacctcgac gcgctcctgg
      241 cggccctgcc gcgggagatc gtcgacgccg tccacgcgtt gcccgagaag gaggcgctga
      301 tcgaggtcgt cctcgacctg ggccggcggc cggaggcgcg cttccccgac agcgaggtca
      361 cgctcctcga tcgcgagatc agcgaggccg atatcgccca tgtcgtcgag cacatcggca
      421 cgttcggcga cgacaaccgg gccgggatcg agcggaccct ccaccggatc agcgcgatcc
      481 gcaaccggag cggcaagatc gtcgggctga cgtgccggat cgggcgggcg gtctacggga
      541 cgatcgacat catcaacgac ttcgtcgaca gcgggaagtc gatcctgatc atgggtcgcc
      601 cggggatcgg caagacgacg atgctccgcg aggcggcccg ggttctcgcc gacgacctcg
      661 gcaagcgggt cgtggtcgtc gacaccagca acgagatcgc cggtgacggc gacatccccc
      721 acccggcgat cgggaaggcc cgccggatgc aggtccggac gccgtcgctc cagcacgagg
      781 tgatgatcga ggcggtcgag aaccacatgc cccaggtgat cgtcatcgac gagatcggga
      841 ccgagctgga ggcccaggcc gcccggacga tcgccgagcg cggcgtccag ctgatcggca
      901 ccgcccacgg caacaacctc gacaacctga tgctcaaccc gaccctgtcc gacctgatcg
      961 gcgggatcca gagcgtcacc ctcggcgacg aggaggcgcg ccggcggcgg acccagaaga
     1021 gcgtgctcga gcgcaaggca ccgccgacct tcgatgtgat catcgagatc caggatcgcg
     1081 agcgggtcac cgtccattcc gacgtcgccg agaccgtcga ctcgatgctc cgcggcgact
     1141 cggtcgcacc agagctgcgc tggcgcgacg aggagggcgt ccaccggagc caggcccggc
     1201 cccggccgtc gcctcgcgag cagatcggtg ccgagcggtt cgccggactc gtcggctccg
     1261 gtcaaccgtg gcggatggag ccgggctggc gtggcgacgg cgggtaccgc ggtggcggct
     1321 atcgcgacgc aggcggcggt ggtggcacgg ccggtggcgc cggtggtgcc gggttccgct
     1381 ccggctaccg gcccggcgcg agcggtggct ggcgccagac ccggggcggc tcgggccgcg
     1441 agacggcccc ccgcggcccc cagtcggagg gtggcgcagc ggacgtcatc ccgggtgagc
     1501 gcttcctgac cccaccggcc ggcggccgcg tcggcggacg gctcgacgat ccgccgttcg
     1561 tcagcgggga gatcgccgat cgtgggccgc tcgaacgcgg cgcgcccgtc gtgcccgaca
     1621 cccgcgctcg cgaggcccgc gagctcgatc gccagaaggc ctggcgcgac caggccggcc
     1681 gtgccctgac ggcgctcaag gccgacgagg gcacggcgcc ggtcaacgcc gacgagctcg
     1741 acgacgacga ggatgcgctg ctgccgctcg ggatcgagga aggggaccac ggcgaggccg
     1801 tgctcgtgcc gggcgggtcg cccctcccga ccctccgtgt cctgccccag gggatcagcc
     1861 ggaagcggct cgaggccgcg atccgcgacc tccagctgcc ggtcctcatc gcccgcgacg
     1921 tggacgacgc agacgtcgtc atgaccctcc gcaacgagta caagcagaag acgccgctgc
     1981 ttcgcgaggc ggaggagcgg gccctgccga tctacgtcct gaagtcgaac accgtgcccc
     2041 agatgcagtc gagcctgacc tcgatcttct cgctcgagat cgacccacgc gaggccgcga
     2101 tgcgcgagac cgaggaggcg atcgggatgg tcctccacgg ctccgagccg gtcgagctgt
     2161 cgccgcagaa cgcctacatc cggcgcctcc agcaccagat ggccgagcgg gcgaacctcg
     2221 tctcgcgctc ccgcggacgg gagccgtacc ggcgggtccg gctctatccg gacgcggcac
     2281 ggccggcgtg gcgttgaccc ggcgatgagc agcgacgccg caccgcccga gggggcgacc
     2341 aggccacggc tcgggcgact ccacccccac aacgtcgtga tcgatgtcgc cgatcatgac
     2401 gccgtcgtcc cgttctggga ggcggcgctc ggctggaccg tccagcggat caatgaccag
     2461 ttcgtcggcc tgcgggcgcc cgccgatgag gcgatcgggt tcgacatcct gttccagaag
     2521 gtgcccgagc ccaaaacctc caagaaccgc gcccacatcg acttcgacgc cgccgacatg
     2581 gaagccgagg tcgagcgcct gctcgggctc ggcgggacga agctcgccga acacaacctc
     2641 ggcgccttcc gctggacggt cgttgccgac cccgaaggca acgagttctg cgtgacgatc
     2701 cggtgagcgc caatcggccg gcggcgatcc tggtcgcgca accggcatga ccgatgtgcc
     2761 ggccgaatgg cgccgcggat tgttccggct ggccaacgcc ctcatcgtct acggggtcat
     2821 cgggatcctg gccgcggcga tcgggatcgc ggcgctgatc ggcgccagtg gtcggatcgg
     2881 ctcgcttggc gagcggatcg tgtcggaggc ggtgagtctc gacgcgatca tcggatcgtc
     2941 ggccgatgtc ctcgatggcg ccgccacgac cgtcggcacc ctcgggccga ccgtcgagcg
     3001 gacatcggcc acggtcagcg ctgccgccgg cgcgctccgc gacatcgaac cccgcctccg
     3061 cgacgtggag acccaggcca acgcattcga gatcttcggg cagcggcccg ttgcatcgct
     3121 cggtcagctc ttcggccaga tcgcgaccga tatctccggc ctcgacgaac agctcgacgg
     3181 aatcgccacg gagatgggtc ggaccaaggc ggcactgacg gcgaacgcgg aatcgctcgg
     3241 tgccctggcg gacttgatcc gcgcctacaa ggaccgcctg aagccagagg ccatcgacgc
     3301 cgggatcgac gaagcacggc gtctgttgct gttcacgttg accctcttca tcggctggac
     3361 ggcggtgcct gcgatcggct cgctgatcgt cggtcgttgg atgcggcggt tggtcgagcc
     3421 ggagcgtgtg ccgccgtccg aatcgcccgg acctgcgccg atgatcgatt ccgccgggtc
     3481 ctgaccgagc agcgtcagcc gcgtcagccg cgcccgccga actcccggtg caacgccgac
     3541 cgggcggcga gatagccgct catgccgtgg acgccgccgc cgggcggggt cgatgacgag
     3601 cacaggtaga tgccgggtgc gcccgtccag tacggatcga gcaacggccc cggccggaag
     3661 atcagctggc ggatgtcctc gatcccggca ttgatgtcgc ctccgacgta gttcgggttc
     3721 tccgcttcca gctcggccgg gccgcgggtc gcccgggcga ggatccggtc gcggaaaccg
     3781 ggggcgaacc gctcgacctg gccctcgatc cggtccgtca tgtcgaccgt cgagccggcc
     3841 ggaacgtggc agtagcccca cgccgtcgtc ttgccggcag gcgcccggct gtcatcccag
     3901 gggctgtact ggacgcacaa cacgaacgga cgatcggggt ggccgcctag ggcgaccgtc
     3961 cgctccgcgg ccgcgacctc gccgagggtc ccgccgaggt gaatggtcgc cgcccgtcgg
     4021 agggcctcgg cgcgccacgg gatcgggccg ccgagcgccc agtcgacctt gaacacgccc
     4081 gggccatagc ggaaccgctc gtacaggcgt cgggtccgga ccggcaggcg atcgccggtg
     4141 atctcgaggg ctgcccgcgg tgtcgtatcg aggatgaccg cccgggccgg cggcagctcg
     4201 gcgaggctgg tgatgagccg gcccgtctcg atcgtcccgc cgagcgaccg caactcggcc
     4261 gccaggccgt cggcgaccgc ggccgagccg ccgcggatca tcggccagcc gaccgcgtga
     4321 gcgtacagcc cgaggaccag gccgaacgac gccgacagcg gacgatggag atcgaccatc
     4381 gaatgagcgg cgagaccggc gagaagcgcc cgggcgggct cgtcgcggaa cgtgatccgg
     4441 gcgagaccag ttgccgacag gagcgcgggc agcccgaacc ggccgagcgc gagcggatgg
     4501 cgcggaacgt ggatgaccgg acggagcagc tcggctccga gcttctcggc gtccgcgaca
     4561 agcggtccga acaggcgtct ccaggccggg ccgtcgcgac ccagcgccgc ggccgtctcg
     4621 gcgaccgaac gctcgacgat cgcggctcgc ccaccgtcca gggcttgccc gaccggcgcg
     4681 tcgggctgga cgatctcgat cccgcgcgcg gcgagatcga tggatcggaa gaacggcgag
     4741 gcgagggtca gaggcaggat cgtcgaacag acgtcgtgac ggaagccggg gagcgtcagc
     4801 tcctcggtcc gggttccgcc accgatcgtc gcggccgcct cgtacacgtg cacggagcgg
     4861 cctgcccggg caagggtgat cgcagcggcg aggccgttcg gtccggctcc gacaacgacg
     4921 gcctccgggc cgccgctcat cgacgcctcc cgatccgagg gctcagcctc cgcctcggac
     4981 ccggctcgcc acccagcggc tcggggcggt cgcggtccgc atcatcgagt ggatggtcgc
     5041 gctgtggcgc aggttgcgaa cgtaccgcca ctgtcgtttc cgatcgacgc acacgacggt
     5101 ggtctcgacg cttggatcgg acgtcccgag atgtcgcgcc agcgcgatca gcgtttcctc
     5161 ccagaaccgg gtctgcatcc ggctcccccc gaggttgtag gcgagatcgt cgagcggatc
     5221 ggacggccgc tcgagcacct ggacctgggc gagggtcacg ccgctctcgt cgaaggccga
     5281 gaaggtgatc catgccgaca gggtatggcc ctcgggcgtc atgaatgtga acgattcgtc
     5341 gtccgcgtag atcaccatca ctccggtcga gagccggacc ggggctccgg gcagcagcgc
     5401 gatctcgagc agcgcgactt ctccgggggc gatccccgcg aggggagcgt agaacttgac
     5461 gctcttcggc cagcatgtcg ggaaccgctc cttccacgtc gcgatgacgt cgcgcggcga
     5521 cgccccgccg tccagcggga cccgaaacgt cttctgccac atctggccaa agccgtggac
     5581 cgggcccgcg acacgccgac cggtgaccgc gtcatccggg ccgaccgcgg tcgtagcgag
     5641 gctgctgacc ggatgcgccc aggctccggt gtccttcggt tccatggcgg atacctccct
     5701 cacgcccgtc cggccgcgag ggccgcattg gcgtcttcgt ggatcccgat cgcctcatcg
     5761 aggcggatca gggagaagat ctcgcggtag tggtcgctca ggccgaccgc gaggagccgc
     5821 tggccctggc gcttcgagcg gaccagcatg gtcacgagga gcccgatccc accgctgttc
     5881 atgtactcga gaccggcgaa atcgaggacg atcgttcgag cgcgcccatc gacggccttg
     5941 gaatacgcct ccatcagggc cggttcgctg cccccggtca catctccgaa gatcgcgacg
     6001 acgctggcgg tcggatcgtc ggtcgaccgg acgtcgaccc tcgtacccgg gtcagccatg
     6061 gtcctgggcg ccttcctcga cgccggcgtg gaccggcacg ggctcgacgg cctggtcgtc
     6121 ttcgagcgtc acgtagtcgg acaggcgggt gattcggaag atctctcgat agtgctcgga
     6181 cagctcggcc ggcaccgcct ccaacatgga gttcatgaac tcgccctcgg cgatcaccca
     6241 gacccccagc gcggtcatcg acagggtgag cagagccagg cgagcacggc gacgctgcgg
     6301 cggatcatgg catccctccg cggccggacg gtcggatcca gatgccgacc gtcctggggc
     6361 tgagcgccga gtcgatcgtc ccggcgatat cgaccttgag cccggcgatg tccacctggt
     6421 cgcgctgccg ttcggcgaac gccgcggccg ttcgctcggc gtcgtagcgc gcccgattga
     6481 acctgtggtc gacgaccgat tggacgcgcc gccggagcgg ctgaaagagg acgaatgcga
     6541 cgagtgtgga cgcggcgacc gaaagtgtct gggcctgggt gacgtcggcc aggatcgtct
     6601 ggagccccac gatcgtggcg gcgaagaccg cgccgagaat gcccgtcacg accgcccagc
     6661 cgatcgtccg gctgatgagc cggtcgatct cgtacaggcg atagcgcgtg acggcgatcc
     6721 cgatcgcgat gggcatcgcg aggatgccgg cgtagggaat cagcgccgag acgagtgggc
     6781 cgtccccggt ccggactgcc atctccacca ggacgcctcc aaacccgacc ccgaggaccg
     6841 ccacggccgc gccgaaccac cggatctgga gacgttcgat gcgatctcct ctccggtagc
     6901 gggtgatcag ccccgcgatg gccagcgcga tgagtgccat caactcgaga ggtatcgcgt
     6961 cgacgaacgg ctggaggtag ggcggccagg cgtcgaagac gatcggactg ccccggactc
     7021 tgccgcttat cgtccaaacc ggcaccgccg aggcgaccag accgatgctc gacaggacca
     7081 cgagcaatcc aaccgggatc ctccagcgag gaccaggaag cgtccccgtc gggaagagca
     7141 gcggcaccca gccggcgatg agggccatgc ccggatacga gaggagcgtt cctccccagg
     7201 tgacgacacg gtagagctgc gggtcgacga acgattccag cgtgtccgcc gtcagccaga
     7261 ccacggcgag aagggcgtag agcgggccgc tcagcatcag caaccggccg atcgtgtggc
     7321 ccggccgccg ccattcgatg atggcgccga caaccgactg caggatgcac gtcccgttga
     7381 agagaacgcc gacgaagacg tctggggtcg cgccgacctt gtagcggagg aggaccacat
     7441 ccatcgccac cgagatcgcg atcgacgcgc cgatggatgt cacgatcatc aggaccgaga
     7501 gcttccccgg ccgccgttcg gcccttccgg tcgtcatcgg cccgaccctg ctcgcagcca
     7561 ggcgatcgct ccgacggggc gcacgacctg ctcgaccttc accgggcagt gcgatcggac
     7621 gagcgcaacc agacgctgac cgaggtcggg gcgaggctgc tgtcgatcac cgcgcgaagg
     7681 tgatctcgga gtcgctcgag atcgacctcg tctcgaacct gtccggcgaa gccgtggacc
     7741 gttagctggg cgtcatagcg agagcggttg aatcgcctgt ccatcgccgc gtgaatgcgc
     7801 cggcggagtg gttggaagag cacgaccgcc gccagcgtcg acgccgcgac ggcaaccgga
     7861 ccgccgccac tcgtgaatgg cgcaaggacg gtctggagga gcgcgacgcc ggctgcgtag
     7921 acgccgacga ggacgagggt cagtaatccg tacgtgagtg ttcggctgat gatccggtcg
     7981 atctcgtaga ggcgataccg caagatcgcg attccgatcc cgattggcat cagcgtggcg
     8041 cttgcgactc ccaggaagtc gagagggttc acgacttcgc cgccgaaccc aagaggaaag
     8101 aggacggcac cgacgccgag cgcgccgagg agccacttca actgggctcg cagcagacga
     8161 tcgccgcgat gccagcggac cccgatggcc gtgaccgcca gcaccagcga caggacgagc
     8221 agagccgttc cgcccaggcc aagcagggtc atgatgcccg gcgtcaggct cagcacgcca
     8281 agcgggttgt ccgggaggcc cggagcaacg ggaccgaccg tgatcgcggc gattcccgag
     8341 atcgcgacct cgccgattgc gatgaacaca accggccccc gccagcgcgt ccccggcaac
     8401 cgcccgtccg ggaacaggat ggccaccagc ggccaggcga ccaggatggc tggaaagatc
     8461 gtgaccgcgg cccaccagcc agccagcgcg cccagcagat ctccgggccc ggccgtcacg
     8521 gcccggatcg cactgatgag gaaacccaga aacacgccaa tcacgagcgg accacccgcc
     8581 tggaggattc gcccgatccg attctccggc cgccgcgacg cgatcaaagc gccaaccgtc
     8641 gcgttggcaa cgccccagat gccgatcagg ccgaagaagg ccaccaattc ggcgcctcca
     8701 ccgccgatcc cgggatgttg cccagcgacc gccgcggtgg caacaagacc aaggaacagg
     8761 gcaatggtga tgagccacgg cgcgaggatc gaccagcgca tcacccggcg ccgcccagcc
     8821 tgggagcccg gtctgaactg gccgcccgca tcatgcacgt ccgaccgagg gccgctccga
     8881 gaacgcgaag gatgcccatg ccgccgcgaa ggtggcctcc acggccacga gcagccaggc
     8941 ctgcgacccg gcccgcagct cctggctcag gcgggcgagc cggcggccgc aatcggccaa
     9001 taccgcgact gcgtccgaat tctcgatcgc gacctgggcg tcgtgccgct gcccgagacg
     9061 accgagctgt atgaggccat ccgggcgggc cgccttgggc atgcgccgtc gtccgagccg
     9121 cggctgccgg ctgccggctg ccgtccaacc cacgattgcg ccgcctgccc ttccgcttgt
     9181 cggtcgcgac cgagagatgg cgaccctccg cgaggctgcc cactcgatcg gtcccgatgg
     9241 atgcatcgtc gtcgtcgaag gcgaggccgg gatcggcaag acgcgactgg ccgccgagct
     9301 cgtcgccatg gtccgcgagg ccggcggcag cgtcctggag gcacgggcgt acgacggcga
     9361 gtccacgatc gccctggccc cgatctcgga gctgatccgg gtcgacctgg atcggccagg
     9421 tgccgaaaca cggttgcaac acgttcgccc agacctgcgg acggaagccg gacggctggt
     9481 ctccgtgccc gaggtgatga tgtcgggcag cctccccgat caagtcgccc acccattcgg
     9541 tgggtcgcgt ctggtggagg gcctcgcgga agtcctgacc gccctcacgg tggcgcggca
     9601 tgcgccgtca cgcagagcga cgtcggcgca gccatcggac gggcgccatc acggtcccga
     9661 gtgccatgct cagggcgggg ctgttccgga cgttcccgac ctgccgccac tgacgacgac
     9721 gatccacgca gaccttcgtc gacgtgaccg tcgggttcgc gaccccgatg gatcgggcaa
     9781 ggttctcgag ggtctgctcc cagaaccggt cgttcatccg attggcgccg aggaggtagc
     9841 tgatctcgat gagtgggtcg ctcgtccgct cgagcgcctg cacctgggcg gtcgtgccct
     9901 gatcgtcgcg cgtggccgag aaggtgatcc atgccgacag cgcgtgaccc tccggcgtca
     9961 tgaaggtgaa cgactcgtga tcagcgtaga tgacgaggac acccgtcgac atcttgatcg
    10021 gggagccggg gatcggcgga acctcgagga gggcgacctc cccggggctg atccccgaaa
    10081 gcggcgcgta gaacgtcgcg cccttcggcc agaacgtcgg gaactcggcc ttccagtgcg
    10141 agatcacggt atcgggcgcg acgtcgacgc cgtccattcg gaccctgaac gtcttctgcc
    10201 acatctggcc gaagccctgg atcgggccgg cgacccgctt gccggtgacc gtgtccgtgc
    10261 cggcgccggt ggccgtcgtc gtcagcctgg cgaccgggcg tgcccatgca tcctgatcgc
    10321 gccccggtcg gggcgtcgtc tgcgtcatct cgacctcctg tcgacctcgt cgtctcaggc
    10381 aaccgcggcg acggcggcct cggtgctgtc atggatcccg atcgcctcgt cgagccgggt
    10441 cagcgagaag atctggcggt aatggtcgtt gaggccgacg gccatcagcc gcctgccctg
    10501 gcgctgggcc cggacgagca gggtcacgag caggccgatg ccgccgctgt tcatgtagtc
    10561 gagtgcgccg aagtcgagga tcacggtccg ggcgccaccg tcgacagccc tggcgtacgc
    10621 atccatgagc ggctgctcgc tggccgatgt gatctcgcct tcgatgtgga gcaggctcgc
    10681 ctcggctacg tccgtcgaac ggacctcgac acggatcccg tcggtcattt cgcgcctccg
    10741 ttgtggcctg gttcgtctac cgtcgaatcg tcgtcgaggg tcatgaaatc ggagagccga
    10801 gtgatccgga agatctcccg gtagtgctcg gacagcccca tcgcccgcac cgcacggttg
    10861 tcccgacggg cttcggccag gagccggacg atgagcgcga tgcccgtgct gttgatgtag
    10921 tcggtcgcgg cgaagtccag ggtgatcggc ccggagccgg tcgctcgcgc ctcgccgtac
    10981 gcgtcggcca tggcggcatc ggcgtcccgg tcgatccggc cgctcaggac gatccgggcg
    11041 ccgtcggccg cggggcggac cgtggccgac aatgggctgg tcattcgccg gagcctccga
    11101 gctcaagggt caggacgacc gtctggcgct cgccctcgcg gatcacctcg acgcggtcga
    11161 ccatctgctg gatcaggaac aggccccagc ccctcggctt ctggagaccg gcgagcttgg
    11221 cgtcgagatc cgggacctcg gcgtccgcca gatccggcac ggtcccgccg agcccggcat
    11281 ccgcgatccg gacgacgagc tccgtcggct gccgctcgat gcggatgcga accgggacct
    11341 ccgggtcgcc cgccgatccg tactcgatcg cgttcatggc cgtctcgctg acggcggttt
    11401 tgagtcgctc caggcgatcg ggcgggaggc cgatcgattc cacggcggtg gcgacgcgct
    11461 ccatcgcgag ccgctcgttg ccgaaggcgc cgggcagctc gaactcggcg atgacgtcga
    11521 ccgtcgtcga cggcggttcg gagatcgctt cgtccgccgt gacggacccg ccgccgaccg
    11581 aacggcgcag ggcgaccatc gtgatgtcgt cctcctgttc ccagccgcga ccggtgaatt
    11641 cgtggaggtg ttcaagcaac tcatcgagca gatccgatcc ggccagctca gacgccatgt
    11701 ccgccttcag ccggggaaag ccgtacatgt cgccgccggg atcgtgggcc tcgatgatcc
    11761 cgtcggagta gagcaggacg ctgtcgcccg gggcgatgtc cgcttccttc tcctcgtaga
    11821 cgagccccgg gaggaggccc agaggcatgc cggtcgcgcg gaattcgacg acaccgccgg
    11881 ccgtccggac gtagggcagg ttgtggccgg cgttcgcgta gacgacgtgg ccggtcgcgg
    11941 gctcgaggac catgtagagg caggtcacga acatcttctc gggcatctcc gccacgagca
    12001 gctcattcgc gcgggcgagg acggcgccgg gggccaccag ccgcggcgcc tccgcgcgga
    12061 ggatcgaatg ggtgcgggcc atgacgagcg cggcggggac gcccttgtcg gtcacgtctc
    12121 caacgacgag gccgatccgc ccgtcgccca ggtcgatgaa gtcgtagaag tccccgccga
    12181 cgacccgggc cgggccgtaa tacgccgcga cctgccactc ggggaggttc ggcagctcac
    12241 gcggcaggaa ctgctgctgg atcagctggg cgactctcag ctcctgctcg tagcgctccc
    12301 gctcggcggc ctcggccgcc tgctcgcgga cgagctgggc gacgcggatc gccggtgcgg
    12361 cctgcgcggc aagcgtcccg agcaggcgcc ggtcgtccga cgagtagtcc tgctcggaca
    12421 ggcgagggcc caggttgagc gttccgatca gctcgccctg ggtcacgagc gggacgacca
    12481 gggcaacgcc ggctccgcgc aggtcctcga ggaccggtga cgcaagctcg aggcgggcca
    12541 gatccacggg gcccggtgcc gactgcagga aggcgaagag gggatcggtc tcggggatgt
    12601 cgatcgccac gccgggacgc tcgtcgacgg ccgggaccgc gggcgccatc cccgtgatcg
    12661 gcgcgggtgc cggcgcgcgg cggccgagca ggcggcggaa cggacgcgag agcgcggtca
    12721 tcggctgcat cctcctcgac gatcgtccgg atatcgttac ggattcgccg cgttccgtgg
    12781 attcgggacg cttcgggccg gcgcggccca gcggccgctc gggtgagccg gtcatcgctc
    12841 ggtctccgat ccgactcgca gccagaccgc ggcattgacc ggtcggaccg cgtcgtccgc
    12901 ggtccggatg agggccgtcc gcaaggtccc gagatcgacc tcgttgcgga gccgctcggc
    12961 gaagccgccg accgttcgct cggcgtcgta gcgggaccgg tcgaagcgcc gatcgacgat
    13021 cgcctggacc cgccgccgaa cgggctggaa caggacgaag gcgacgagag tcgaggccgc
    13081 aacggcaagg gtcccaccct gggtgacgcc tgacagcgcc gcctggagcc cgacgagccc
    13141 gacaacgaac accgcgccga ggacgccggt cacgaccgcc cagccgatcg tccggctgac
    13201 gatccggtcg atctcgtaca accggtatcg caggatggcg atcccgatca cgaccgggag
    13261 cgcgaacagc gtgaggatcg tgaccgtgtt gatgaccggg gacaggtcgg agctcgaatc
    13321 cgggatgaac gacaccggaa ggaccacggc cccgagaccg acaattgcgg cgagccattt
    13381 gatctgctga cgttggaccc gatcgccgcg atggaatcgc agccacacgg cgatggcgcc
    13441 agcgccgaag cagacgatcg tcgtgacgaa gaagaacgtc tgaatggccc cgagcagggg
    13501 atcagggccg gcttggtcgg aggcccccgc ctgggacccg tcgctcggcg ggagcctgag
    13561 gaacgcgccg agggtccacg cgaccacatt cgcgatcgtg agccggacca cccagcggaa
    13621 gcgacctgat ggcaggtgtc cgtccgggaa gacgagcggg accccgatca acgcgatcat
    13681 gaacgggtac acgaacaggg tgctccccgc gatcagggcc gctcccgacg acggccaggg
    13741 tggttcctgc agagccccga ggctggcata ggtccccgtc gccatggccg cgctcatcag
    13801 ggttccggcg gcgagcagga ggaggccgat cctgttcccg ggcactcgcg tgtcaagcag
    13861 ggctccgacc gccgcaaacg aagcaactcc gatcgtgaag atgacgacgc cgctgatgtc
    13921 gccggccggg tcgatcaatc gagtggccag gatgacgacg atgtcgaggc ccgcgatccc
    13981 ccaggtgagc tgcgcccgct tcatcggtcg gcgggtccgg tccgcagcca gaccgcgggg
    14041 ctcacgggcc gcacggcgtc acctgccgtg gcgagcagcg ctgtccggag cctcgccaga
    14101 tcgacctcgt tgcggacttc ctcggcaaac gcgtccaccg tccgctgcgc gtcgtaacga
    14161 gcacggtcga atcggcggtc cagggctccc ttgaccctcc gtcgcatcgg ttggaagagc
    14221 gctgccacga caagggtcga gcccgcgacc gcgagggtgt tgccgccgat gacctgcgac
    14281 aagacccctt ggagcgcgat gaggacgacg ccggccaggg tcagcagctt gaccttgcga
    14341 cgctcctcgg ccgttgcccg ccggaatcga acgacgagcg aggccatgat cggcgcgcca
    14401 cccccgatgt tcgcggcgag gatcgcgcgg gactcgagca ccagcgcggt cgcggccagc
    14461 gcgatcgccg tcgggccggt gaacctggag tcgcctggag agatcgtgct cgtcaacgcg
    14521 gtcgcgcctc ccgcctgcgc atccagacca agctcgacgt cggtcgcatg gttctggaac
    14581 atgccaccga tagggcgagg atgatcgtcc cgagatcaac ctggtcgcga agagcatccg
    14641 agaaggtatc cacggtgcgc tgggcgttgt agctcgcgcg atcgaagcgc tgatccacga
    14701 catgctggat ccgcctccga acccgctgga acagcgccgc ggcggtcagg gtcgaaccgg
    14761 ccaccgcgaa ggtgttctcg tgggtgaagg ggtcgaggac cgcctggagg gcaaacaccg
    14821 aggccgcgaa cactccgacc aggacggccg tcacgatcgc ccacgaaatg gtgcgcgaga
    14881 tgatccggtc gatctcgtag aggcggtacc ggaagaccgc gatgccgatc gcgagcggga
    14941 cgcatgggaa cgcgacgatc gcaccgagcc atgccagcgc gctgtcggtc gtggccggcg
    15001 ccaggctggt gaccacgaag ccggacacga cggccagcgc gacgaaggcg agagcggtgg
    15061 tgatccagcg gagctgctgg cgctcgatgc cgctggcgcg tcggaatcgg accagcagcg
    15121 agatcgtcgc gccgatcatg agcgcgacga tcgggaggat cgcggtgtcc ggggtgatca
    15181 cctgccagat cgcgagatcc ggcaggacgg ccgccgggtt ccggaccgga acgctggccg
    15241 cggaaccccg gatgctcacg ctggacatcg gcatgacggt gcccgcgcca accgtgagca
    15301 atccgccgac gatggcgagg cgcgtgatcc ggcgccatgg cccacccggc agccggccgg
    15361 acgggaagac gatcgcgagc acggcgaaca gcaggaacag ggccgggccc gaggccgcac
    15421 cgatcacggc gaggccgacg gtgatcgggt cgaggttccc ggcggcgaac tgtgccggtg
    15481 ttaggtcgac ggacatcgtc acgatcgcga agccccaacc gagcccgaga aggatccagc
    15541 caacggagtt ccgcggccgg cggatggcca gcagcgtgcc gacggccgcg ttcggcagga
    15601 agaagagcag accgaagcca ctcggcgtgg cgacgattgc gcccgcgatc aggagcacgc
    15661 ccaacaggac ccgcgccggc cggccgagcg ggtagggggt cgcgacgggg gcgccggacg
    15721 tggggacaat gcgtgggcga gtcacagtca tggcaccgga atgctggggc gacgccgtcc
    15781 gggaaacgtt acgggatcac ccagtgtcgg ggtaccgtta cgcgcgatgt cccgcccgcc
    15841 actcccgtcg gcccgctcgc gctcggggct cgaggtgcgc gtgctcggac cgctcgacgc
    15901 gcgcgtcgac ggcgcgtccg tcgtcgtcga cacgcgcaag gcgctggcga tcctcaccct
    15961 gctcgcggtc gagcgccggc cctacgctcg cgacgagctc gccgccatgt tctggcccga
    16021 gtccgacgag gagtcggcgc ggggcgccct gcgccggacg ctctcggtgc tccgtgccgg
    16081 gctcggtgag cgatggttgc gcgtggatcg gaccgtcatc gcgttggaca tggccggcgt
    16141 gcagctcgat ctcgcgacgc tcgaaggggc tcagggctcg agtgatcgca ccctcctgag
    16201 tcatgccgcc gaagcagccc gcggcccgtt tctcgccggc ttctcgcttc gtgacagccc
    16261 ggacttcgac gactggcggg cgacgcaggg ggcagccgtc gagcgcatgg tcgccaccgt
    16321 cctcgaccgc ctgtccgccg ccgccgaatt ggccggcgac ctcgtcggcg cggtcgccgc
    16381 cgcggcgcgc cgggtcgacc tcgatgccct cgacgaatcc gcccagcgtc gcctgatgac
    16441 gctccttgtg cggtcgggcg accgagccgg cgcgatccgt caataccgga catccgttgc
    16501 gattctcgag cgcgagcttg gcgtgagccc gcttgccgcc acgaccgatc tctacgaatc
    16561 gatccgggat tcacgccttg atccggtact cgcccaggtg gcggcagcgc ccgtgccggt
    16621 tgcccgacgg ctgccgctgg tcggtcgcga cgccgagctg gcggcgctcc tccggaccca
    16681 tgccgactcg accccgtcgg gtcgcctggt tctcgtgacc ggtgaggcgg gcatcgggaa
    16741 gacgcggctc ggcgaggccc tggcagacat cgtcgaaggc aacggcggaa cgacgctcct
    16801 cgcccgcgca tggtcagccg agacggccat tccgtacggg ccgatcgtcg agctgcttcg
    16861 cgccgggatc ggtcgtccgg atgcatcgga ccgtctcgct cggctgccgc cgcgcgcgct
    16921 ggtcgatctc gagcggctcg tgacgctgcc gccgacgctg gccgtgcggg cccccgttgg
    16981 ctccacggcg gcggatcccg gcggacccca ggtggtcgcg accgaggacc gctcggccgc
    17041 ccgcgtccgg atgctcgatg cggtggcgga ggccctgagc gcgctcgtcg gaggaccgat
    17101 tcccggcgtg atcatcgtcg aggacgtcca atgggccgac gacgcgacgc gcgaggccct
    17161 gacctggctg gcccggcgtc tcaacgaccg atcactcatc ctcgtgctgt cgtggcggcc
    17221 ggaggacctc gatgaccgcg ggctcgcatt cgcggctcga ctcgaggagg tcgcggcgat
    17281 cgatccgctg cgactgcagc ggctcgaccg atcggcggtg acagccatgg tcgaggccgt
    17341 gccgaccgcg aaccgtgcgg ccatcgaagt cgacgtcgtc gtcgaagaat ccgaaggaat
    17401 ccctctctac gtcgtcgagg ccctggcggc cgcttcggga cggtcgtccg acgggccgca
    17461 ccacggcgtc cgggccctcc tccgggaacg cctggcgacc gtcggcgaga tggcgagtca
    17521 ggtcctggcc gcggcggccg tgatcggacg ttcgttcgac cttgccacgg tccgcgcggc
    17581 cagcggccgg accgaggacg agacggtggc ggcgctcgag gagctcgtcc gacgagggat
    17641 cgtccgcgaa ctcggcgatg gcggaagcct cgcattcgac ttcgcgcatg cgaagctccg
    17701 cgacactgcc tacgagggca cgagcctcgc gcggcggcgg ctcctgcatc ggcgtacggc
    17761 cgagctgctc cggtccatgc ccggcgcggc tcaggatcgc ggtcggctgg cccagatcgc
    17821 ccgccacgaa cggtcggccg gccgggatgc agacgcggcc gaggcgtttc gcgaggccgg
    17881 ggtccgatcc cggtccgtgt atgcaaatcg cgaggctctc gagcacttcg agacggccct
    17941 cgccctcggc catccggata tcgtcggtct ccagctgacc atcggcgagc tccggacgat
    18001 ggtcggtgac tacgccggtg cctcggccat gctcgaggcc gccgccgccg tcgccagcga
    18061 gcgcgacctt ccagccatcg agatgtcgct cgggctgatc cacgtccggc gcggcgatct
    18121 cgacagtgcc gccagtcatc tcgacgccgc gatcgaatcg ctcgaggacc gcgacggtgc
    18181 ggagcgcgcg atgctcgcgc ggatcctcgt ggagcgcggc ctggtcgccc tgcgggcccg
    18241 ggacctcgaa cgcgcccgcg gctgcgccgg cgatgcgctc gtcatggccg aggaagcgaa
    18301 ggatccggcc gcggtggggg ccgcccaccg gctgctcggt ctcgtcgccc gggagcgcgg
    18361 ggatctggtg gtcgcccgcg ccgagctcga acgaagcgtc gccctcgctt ccggggaccc
    18421 ggacggggcg accgcgatcg cggccggcaa ttccctggcc ctcgtcgaag ctgcggccgg
    18481 tgatcgtgcc gccgcgatcg atcggctcga gagggcgctc ggggcatgcc aacggacggg
    18541 cctgcgccac ctcgaggcag ccgtcgagaa caacctcgcc gatcagctcc atgctgtcgg
    18601 acgcgtcgaa gacgcgatgg accacctgaa gcgggcggtc gtcatcttcg ccgacgtggg
    18661 tggcacgccc ggcgagctgg agcccgagct ctggaagctc gtcgagtggt agggggctga
    18721 ggcgacgtaa gtgctcagcc gcccaacgtg ccgcggtcga accagatccg ctccccgagg
    18781 aaccgttgcg tgacggggtc ccaaggaaac aggatcagga cctggagcga gaccggcagc
    18841 ccgctcgccg gcgcaccgcc ccacggcccc tcgttcgtgc cggtcagggt cgcgacctcg
    18901 aacacgccct gcgg
//
LOCUS       DATAIW010000420         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_88931, whole genome shotgun sequence.
ACCESSION   DATAIW010000420 DATAIW010000000
VERSION     DATAIW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88931"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VHM48_12650"
     CDS             complement(<1..642)
                     /locus_tag="VHM48_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2756310.1"
                     /translation="MPVLRSRLDPASPETRANHDAMAALVADLRARQATVADRGAGGD
                     DRSIARHRERGKIPVRERIDRLLDPGSSFLELSPLAATGLYDDDAPSAGIVTGIGTIE
                     GTTCVIVANDATVKGGTYYPMTVKKHLRAQEIALENRLPCVYLVDSGGAFLPLQADVF
                     PDRDHFGRIFYNQARMSGAGIPQVALVMGSSTAGGAYVPAMSDETVIVKGTGTI"
     gene            849..1202
                     /locus_tag="VHM48_12655"
     CDS             849..1202
                     /locus_tag="VHM48_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756311.1"
                     /translation="MTIETRGGECATGPCSRLVTIEADGRIHEVIPEDRVVGTVPTPL
                     LDGLQVEMARANFPLLQSKPFRGECPTAVDGQETVYTFHVQAGDEEIASCKVAIDENH
                     PLLRAVAAALAAAGP"
     gene            1255..1578
                     /locus_tag="VHM48_12660"
     CDS             1255..1578
                     /locus_tag="VHM48_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2756312.1"
                     /translation="MSMAGRMLRHSRKRAGLSQRELAAGVGIPQETIARIESGRVDPR
                     VGTLDRLLEGCGFGLEHLPRLGIGIDRTVIRRLIELTPSERLELGIANDRAMVERWQI
                     RRVAE"
     gene            1575..2081
                     /locus_tag="VHM48_12665"
     CDS             1575..2081
                     /locus_tag="VHM48_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756313.1"
                     /translation="MSEEPIFQPDEILRRLVDANVKFIVVGGLAAQAHGSTSLTNDLG
                     ICFARDGDTLRALATVLEEIMAVRRGLPNDAPPMPPLDVRTLRAGGLFTLSTRFGDVD
                     LLANPDPGFDYETLVGAAIAATTAGVRVLAAGLDDLMAMKRAAGRPKDRIELEILGAL
                     REELDRRG"
     gene            complement(2078..2440)
                     /locus_tag="VHM48_12670"
     CDS             complement(2078..2440)
                     /locus_tag="VHM48_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756314.1"
                     /translation="MNPRRDWLAGCTVGAWTGFALVYAPVVGAVLFVAFAVPAAIGRS
                     LAAIGGLLVGSGAVMLLMIALANWNCAGLFAGNDSGCTPPDLTGWLVAGAAMALLGGV
                     LTVLAIVGSRRRASAGRP"
     gene            complement(2437..>3306)
                     /locus_tag="VHM48_12675"
     CDS             complement(2437..>3306)
                     /locus_tag="VHM48_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2756315.1"
                     /translation="ITGGTGFVGQRVAAQLRRAGHVAVVLGRADDPAAAFAGCDAIVH
                     CAGVNREIGSQTYRAVHVAYTRSVVAAARRAGVRRIVMLSFLRARPDCGSGYHESKWA
                     AEEIVRGSGLAWTILKAGVIYGRGDHMLDHLSRAFHSFPIFGLVGFRSRPVRPVAVDD
                     VARILVAAAIGDERLARGTFAVLGPDELLLSAAVRRVADVAGRRPWFVPLPIAGQRLV
                     ASVAELLMTVPLLSRAQVRILAEGIVEPLPAADGLPVDLLPTTRFDEASIRAGLPPSG
                     GFGRRDLRWCAGR"
BASE COUNT          493 a         1188 c         1188 g          437 t
ORIGIN      
        1 gatcgtgccg gtgcccttca cgatgacggt ctcgtcgctc atcgccggga cgtacgcacc
       61 gccggcggtc gatgagccca tcaccaacgc gacctgcgga atgcccgcgc ccgacatccg
      121 ggcctggttg tagaagatcc ggccgaagtg gtcgcgatcg gggaagacgt cggcctggag
      181 cggcaggaac gcgccgccgc tgtccacgag gtacacgcac ggcagccggt tctcgagcgc
      241 gatctcctgg gcccggaggt gcttcttgac ggtcatcggg tagtaggtcc cgcccttgac
      301 ggtcgcgtcg ttggcgacga tcacgcaggt cgtcccctcg atcgtgccga tgccggtgac
      361 gatcccggcg ctcggcgcgt cgtcgtcgta gagccctgtg gccgcgagcg ggctgagctc
      421 gaggaacgac gaaccgggat cgaggaggcg gtcgatccgc tcgcggaccg ggatcttgcc
      481 gcgctcccga tgacgggcga tcgagcgatc gtcgccgccc gcgccacggt ctgcgacggt
      541 cgcctgacgg gcgcgcaggt cggcaacgag ggcggccatc gcgtcgtggt tggcgcgggt
      601 ctccgggctg gccgggtcga ggcggctccg caggacgggc atggcgccaa ggataggtcg
      661 gacggcccgg ccggaaccga ctcccgccgg acggcgtcag cgcggccatg acgaccgtgc
      721 gcgcgctcgc cccactggct ctcgccctcc tgttggtcgg ctgctggtcc gaggcgcacc
      781 gcccgtctcc gttgccgtcg gcgcatcctc tggcgacgac ggcgccgccg caggagccgc
      841 tcgcactcgt gacgatcgag acgcgcggcg gcgaatgtgc gacggggccg tgctcgcgcc
      901 tcgtcaccat cgaggcggac ggtcgtatcc acgaggtcat tcccgaggac cgggtggtcg
      961 gaaccgtccc gacaccgctg ctcgatgggc tccaggtcga gatggctcgg gcgaacttcc
     1021 cgcttctcca gagcaagccg ttccgtggcg agtgcccgac cgccgtcgat ggccaggaga
     1081 cggtctacac gttccacgtg caggcgggcg acgaggagat cgcgtcctgc aaggtcgcga
     1141 tcgacgagaa ccatccgctg ctccgcgccg tcgccgcggc gctcgccgcg gccggtcctt
     1201 gacgccggcg gccacgacct tgatgatgtt tgtgtcatca ggaggcgacg ttcgatgtcg
     1261 atggccggtc gaatgctccg tcactcgcgc aagcgggcgg gcctgagcca gcgcgagctc
     1321 gcggccgggg tcgggatccc ccaggagacg atcgcccgga tcgaatcggg ccgggtcgac
     1381 ccgcgcgtcg ggaccctcga ccggctgctc gagggctgcg ggttcggtct cgagcacctg
     1441 ccccggctcg gaatcggcat cgaccggacc gtgatccggc gcctgatcga gctcacgccg
     1501 agcgagcggc tggagctcgg gatcgcgaac gaccgcgcga tggtcgaacg ctggcagatc
     1561 cgacgggtgg cggaatgagt gaagagccga tcttccaacc cgacgagatc cttcgccgac
     1621 tggtcgacgc aaacgtgaag ttcatcgtcg tgggtgggct ggccgcacag gctcacgggt
     1681 cgacgtccct gaccaacgac ctcggtatct gtttcgctcg agacggcgac acgctccgcg
     1741 ccctcgcgac cgtgctcgag gagatcatgg ccgtccgccg cgggctgccg aacgacgctc
     1801 cgccgatgcc gccactcgac gtccgcaccc tcagggccgg cggcctcttc acgctctcga
     1861 ccagattcgg cgacgtcgac ctgcttgcga accccgatcc aggcttcgac tacgagacgc
     1921 tggtcggggc ggcgatcgcg gcgacgaccg ccggcgtgcg agtcctggcg gccggtctcg
     1981 acgacctgat ggcgatgaag cgcgcggccg gccggcccaa ggatcggatc gagctggaga
     2041 tcctcggcgc cctccgcgag gagctcgacc ggcgcggcta gggccggccc gccgatgcgc
     2101 gccggcgcga cccgacaatc gccaggactg tcaggacgcc gccgagcaga gccatcgcgg
     2161 ctccggcgac gagccatccg gtcaggtccg gcggggtgca gccgctgtcg tttccggcga
     2221 agagcccggc gcagttccag ttcgccagcg cgatcatcag gagcatcacc gcccccgagc
     2281 cgacgagaag gcctccgatg gcggccagcg agcggccgat cgccgccgga accgcgaacg
     2341 cgacgaacag gacggctccg acgaccggcg catagacgag ggcgaagccg gtccacgctc
     2401 cgaccgtgca gccggccagc caatcccgtc gtgggttcat cggccggcgc accagcgcag
     2461 gtcgcgccgg ccgaacccgc cgctgggagg gaggccggcc cggatcgacg cctcgtcgaa
     2521 ccgtgtcgtc ggcagcaggt cgacgggcag gccgtcggcg gccggcagcg gttcgacgat
     2581 cccctcggcg aggatccgga cctgggcacg cgagaggagc gggacggtca tgagcagctc
     2641 ggcgacgctc gccaccaggc gctggcccgc gatcggcagc ggcacgaacc acggccgtcg
     2701 acccgccacg tccgcgacgc gacggacggc cgccgacagg agcagctcgt ccggcccgag
     2761 gaccgcgaac gtgccccgcg ccagccgctc gtcgccgatc gccgccgcga ccaggatgcg
     2821 ggcgacgtcg tcgaccgcca ccggtcggac gggccggctg cggaatccga ccaacccgaa
     2881 gatcgggaac gagtggaacg cgcggctgag gtggtcgagc atgtggtcgc cgcgtccgta
     2941 gatcacgccc gccttgagga tcgtccaggc gagcccggac ccgcggacga tctcctcggc
     3001 cgcccatttc gactcgtggt agccggatcc gcagtcgggc ctggcccgca ggaagctcag
     3061 catcacgatc cggcgcacac ccgcccgccg cgccgccgcg acgaccgacc gcgtgtacgc
     3121 gacgtggacc gcccggtacg tctgcgaccc gatctcgcga ttgaccccgg cgcaatggac
     3181 gatggcgtcg cagcccgcga aggccgcggc cggatcgtcc gcccgaccga ggacgacggc
     3241 gacgtggccg gcgcggcgga gctgggccgc aaccctctgt ccgacgaagc cggtcccccc
     3301 ggtgat
//
LOCUS       DATAIW010000421         6363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_280961, whole genome shotgun sequence.
ACCESSION   DATAIW010000421 DATAIW010000000
VERSION     DATAIW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280961"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VHM48_12680"
     CDS             complement(<1..1109)
                     /locus_tag="VHM48_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756316.1"
                     /translation="MTSDRAIAVVERLFREEQGRAVATLIRVLGDFDLAEEAVQEAFI
                     TALETWPARGVPDNPGAWITTTARNRAIDRLRRKRVLAEKAEILGRQTLIEEELAAID
                     PDAALDGADPMNPIVDDRLRLIFTCCHPALAMDARVALTLRTLGGLSTPEIARAFLVP
                     EATLAQRLVRAKRKIRDAGIPYRVPDDHALPERLDGVLRVLYLVFNEGYGASKGDRLI
                     RRELSAEAIRLVRVLATLMPDEAEVLGLLALMLLHDARRETRTGPAGELVLLEDQDRG
                     RWDRARIDEGQALLERAMRMRRVGPYQLQAAIASLHDEAPSSDATDWAQIAALYRILG
                     DMQPSPVVELNRAVAVAMADGPDAGLAIVDRLSAAG"
     gene            complement(1183..1581)
                     /locus_tag="VHM48_12685"
     CDS             complement(1183..1581)
                     /locus_tag="VHM48_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2756317.1"
                     /translation="MRYLLLIYTEETDVAPPDDVAAASHAAYGAFTADIKARGLFQAG
                     EALTPTSTATTVRVVDGETVTTDGPFAETKEALGGFYLIEARDLDEAIETAATIPGAA
                     FGSIEVRPIWELPAEYAMAEAAAAGAGGAG"
     gene            complement(1664..2851)
                     /locus_tag="VHM48_12690"
     CDS             complement(1664..2851)
                     /locus_tag="VHM48_12690"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HEX2756318.1"
                     /translation="MLNQPEPACLVIADISGYTGFLAGAELDHAQDILADLMGTIVGG
                     LRPAFRLAKLEGDAAFVYAITELVDAAQLQDTIERCYFVFRRRLRDVQQASTCDCNAC
                     TLVPNLDLKVVAHHGRIVRQKFGSWEELVGSDIIVVHRLLKNHVPEATGFSAYAMYTD
                     ACVTAMEIDDPVAAGFAEHVEAFDGVGEIRSWVRDLHAAWDAELERAQLVVQSRDIAL
                     TFEAVFQAPAALVWDYMTSPIRRPKWQHGVTGVIEAAGTAGRRGIGTVNHCIHGKDAI
                     IEEVMDWQPYDHATYRSLLPIPNVPKLLSTYLFTDLGDGRTRVELRFGKPRSAKDRAI
                     AMPLMPMIDEMVTDGLAHLRPIVEAAARAAEASAPPEPDVPAGQDRNLREPIARVGQA
                     RPS"
     gene            3257..4753
                     /locus_tag="VHM48_12695"
     CDS             3257..4753
                     /locus_tag="VHM48_12695"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX2756319.1"
                     /translation="MATRKEILEIGGREVSISNPDKVYFPEPGYTKMDLVSYYLAIAD
                     GALRGAGGRPMALKRFVDGITKEPFFQKRAPDNTPDWIRTAELTFPSGRTADEIVLDE
                     AAGLAWVVNLGCIDLNPHPVRAEDLDHPDELRVDLDPVPGIEWAQIREVALVCREALE
                     AVGLVGWPKTSGSRGIHINVRIEPRWTYAEVRRSALALARDVEARAPSIATSKWWKEE
                     RHGVFLDYNQNAKDRTVASAYSVRPLPDARVSMPLRWAEVADVVAEDFTIATVPAIYA
                     ERGDAAAGIDEARGSLDALLELSARHEATGQGDAPWPPNYAKQAGEPPRVQPSRARRP
                     KSEYEPGRGVEGGPPPEVAAERAAAVAAGDPNAGLPTEWEGSAPRGAPGGSRPTPTGR
                     RRSSIPVIEISRAASKKEALEGLDRWKARHPEAAAALEPADILTDGMRGRSSLWYRVR
                     VNLIHVPEADRPAQEALDPDYDPWAGYEWPDRSGQLERAPRKQSTRGS"
     gene            4820..5848
                     /locus_tag="VHM48_12700"
     CDS             4820..5848
                     /locus_tag="VHM48_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2756320.1"
                     /translation="MASTRVESSVIAVSWIPSEAIEGPSKVPFELGVTHYDQPPPDHL
                     DDLEDLRRTDRFREANELRAFIEVEDGRIVGHGHLGQGHIGATTVRVGPAAIRFPAVH
                     LPDIRPEPVVGSTSVRFVQTVGGRMGLPTPRPVPHKPFVQFWPSIAWTTLALTINADG
                     SSSHELIGASPFPRHWVYDHEGNLVQKSGVIDFNKWFNDSFGDRTPWGEADSPAVVAS
                     VESALERRLSTTIMRSGAKPKIRTLSAGETLIEQGAAGTQMYVILDGMFVVEVEGNEV
                     AEIGPGAVVGERAALEGGTRTATLRARTRARVAEATSDELELEELGALATTHHAEGDR
                     PEATSEQP"
     gene            5912..6055
                     /locus_tag="VHM48_12705"
     CDS             5912..6055
                     /locus_tag="VHM48_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756321.1"
                     /translation="MPADLVDRLVAVGAITTLEDGRDDSRDEPVASTAQAPPTSRRSA
                     GSS"
BASE COUNT          956 a         2207 c         2295 g          905 t
ORIGIN      
        1 ccggcggcag agaggcggtc gacgatggcg agccccgcgt ccggtccgtc ggccatcgcg
       61 accgcgaccg cccggttgag ctcgacgacc ggcgacggct gcatgtcgcc gaggatccgg
      121 tacagcgccg cgatctgggc ccagtccgtg gcgtcggacg aaggcgcctc gtcgtggagc
      181 gacgcgatcg cggcctggag ctgatacggc ccgacccttc gcatccgcat cgcccgctcc
      241 agcagggcct ggccttcgtc gatccgggcc cggtcccagc gcccgcgatc ctggtcctcg
      301 agcaggacga gctcaccggc cggccccgtc cgcgtctcgc gccgggcgtc gtggaggagc
      361 atcagggcca gcagcccgag gacctcggcc tcgtccggca tcagggtcgc gaggacccgg
      421 acgaggcgga tcgcctcggc cgacagctcg cgccggatga gccggtcgcc cttcgatgcc
      481 ccgtagccct cgttgaacac gaggtagagg acgcggagga ccccgtcgag ccgctccggc
      541 agggcgtgat cgtccggcac ccggtaggga atgccggcgt cccggatctt ccgcttggca
      601 cggaccaggc gctgggcgag agtggcctcc gggacgagga atgcgcgggc gatctccggg
      661 gtcgagaggc cgccgagcgt ccgcagcgtc agcgcgacac gggcgtccat cgccagggcc
      721 ggatggcaac acgtgaagat caaccgaagc cggtcgtcca cgatcgggtt catcggatcg
      781 gcgccgtcga gggctgcgtc cggatcgatc gccgcgagct cctcctcgat cagcgtctgc
      841 cgacccagga tttccgcctt ctccgccagc acccgtttcc ggcggagccg gtcgatcgcc
      901 cggttgcggg cggtcgtcgt gatccaggcg ccggggttgt ccggcacgcc gcgggccggc
      961 caggtctcga gggcggtgat gaacgcttcc tggacggctt cctcggcgag atcgaagtcg
     1021 ccgaggacgc ggatcagggt tgccacggcg cgcccctgct cctcgcggaa caggcgctcg
     1081 acgacggcga tcgcgcggtc actggtcaag agggtgggcg ggtggaggca gcggcggtga
     1141 cgcggacagc tgatggagga cgggcgcggc gatcgtcggg cgtcagcccg cgccgccggc
     1201 tcccgcggcc gcagcctcag ccatcgcgta ttcggccggc agctcccaga tcgggcggac
     1261 ctcgatcgac ccgaacgccg cgcccgggat cgtcgccgcc gtctcgatcg cctcgtcgag
     1321 atcgcgcgcc tcgatcaggt agaagccgcc gagggcctcc ttcgtctctg cgaacgggcc
     1381 gtcggtcgtg acggtctcgc catcgaccac ccggacggtc gtcgcggtcg aggtcggggt
     1441 cagcgcctcg ccggcctgga agaggccgcg cgccttgatg tcggcggtga acgctccgta
     1501 cgcggcgtgg gacgccgcgg cgacgtcgtc cggcggagcg acatcggtct cctccgtgta
     1561 gatcaggagc aagtagcgca tcgggcggtc tcctcgggct gtccggcgcg ctcgaccgcg
     1621 ccgctattgg aatgacgaat gggcaggccc gcgatcgaca gcgtcacgac ggacgggcct
     1681 ggccgacccg ggcgatcggc tcacgaaggt tccggtcctg acccgccggc acgtccggct
     1741 ctggcggggc cgaagcctcg gcagcgcggg ccgcggcctc gacgatcgga cggagatggg
     1801 ccaaaccgtc ggtcaccatc tcgtcgatca tcggcatgag aggcatggcg atcgcccgat
     1861 ccttcgccga gcgtggcttg ccgaaccgga gctcgacccg cgtccggccg tcgcccaggt
     1921 ccgtgaagag ataggtgctg aggagcttcg gcacgttcgg gatcggcagg agcgagcggt
     1981 aggtcgcgtg gtcgtacggc tgccagtcca tcacctcctc gatgatggcg tccttgccgt
     2041 gaatgcagtg gttgaccgtc ccgatcccgc gccggcccgc cgtccccgcc gcctcgatca
     2101 ccccggtcac gccgtgctgc cacttggggc ggcggatggg cgaggtcatg tagtcccaga
     2161 ccagggccgc cggcgcctgg aagaccgctt cgaacgtcag ggcaatgtcc cgcgactgga
     2221 cgacgagctg ggcgcgctcg agctcggcgt cccacgccgc gtggaggtcg cggacccagg
     2281 accggatctc gccgacgccg tcgaacgcct cgacgtgctc ggcgaacccg gccgcgaccg
     2341 ggtcgtcgat ctccatcgcg gtcacgcatg catcggtgta catcgcgtag gcggagaagc
     2401 ccgtggcctc ggggacatgg ttcttcagga gccggtggac gacgatgatg tcgctcccga
     2461 cgagctcctc ccatgacccg aacttctggc ggacgatccg cccgtgatgg gcgacgacct
     2521 tgaggtcgag gttcgggacg agggtgcagg cgttgcagtc gcacgtcgac gcctgctgga
     2581 cgtcgcgaag gcgacggcgg aacacgaagt agcaccgctc gatcgtgtcc tggagctggg
     2641 ctgcgtcgac cagctccgtg atcgcgtaga cgaaggccgc gtcgccctcg agcttggcga
     2701 gtcgaaaggc ggggcgcaga ccgccgacga tcgtgcccat caggtcggcg aggatgtcct
     2761 gggcgtggtc gagctccgcg ccggcgagga agccggtgta gccgctgatg tcggcgatga
     2821 cgaggcaggc gggctcgggt tggttgagca tgacggcggg atccctcgag aggcgtccat
     2881 catcgacggt cgggcgtcac gtgtcacccg tccgcgtcgt gggttcgacg cgcgcgcggg
     2941 ttcggatcat ggttcggcac gccatcgagg cacgccatcg aggcacgccc tcgaggcacg
     3001 ccctcggccc gatgttcgag gtgcattgtt ttcggcacga agcaaccggg ccgcctggag
     3061 tccggtctct ccgaggccta tcacgcatcg tccggaccgg agtcgcggtc gaagctgctg
     3121 gtttcgtcgc caacgatgtc cgtcgggcat caatcggccg gcgctcggcg gcgtcccgcc
     3181 gaattcaatg tctgtcgagc atcagacgct tgacagctgc cacgccctcc ttgcgatcgg
     3241 ccgctagcat cccaccatgg cgacccgcaa ggagatcctc gagatcggcg gccgggaggt
     3301 ctcgatctcg aacccggaca aggtctactt ccccgaaccg ggctacacga agatggacct
     3361 cgtctcgtac tacctcgcga tcgccgacgg ggcgctgcgc ggcgcgggcg gccggccgat
     3421 ggcgctcaag cgcttcgtcg acgggatcac gaaggagccg ttcttccaga agcgggcgcc
     3481 ggacaacacg cccgactgga tccggacggc agagctgacg tttccttcgg gccggacggc
     3541 cgacgagatc gtcctcgacg aggcagcggg gctggcctgg gtcgtcaatc tcggctgcat
     3601 cgacctcaac ccgcacccgg tccgggccga ggacctcgac catcccgacg agctgcgggt
     3661 cgacctcgac ccggtgcccg ggatcgagtg ggcgcagatc cgggaggtcg cgctggtctg
     3721 cagggaagcg ctcgaggccg tcgggctggt cggctggccg aagacgtcgg gctcacgcgg
     3781 gatccacatc aacgtccgga tcgagccacg ctggacgtat gccgaggtcc gtcgctccgc
     3841 gcttgccctg gcccgcgatg tcgaggcccg ggcaccgtcg atcgcgacga gcaagtggtg
     3901 gaaggaagag cgccacgggg tcttcctcga ctacaaccag aacgccaagg accggaccgt
     3961 ggcgtccgcc tactccgtcc gaccgctgcc cgacgctcgc gtctcgatgc cccttcgctg
     4021 ggcggaggtc gccgacgtcg tcgccgagga cttcacgatc gcgactgtcc cggcgatcta
     4081 cgccgagcgg ggcgacgccg ccgccgggat cgacgaagcg cgcgggtcgc tcgacgcgct
     4141 cctcgagctg agcgcccgcc acgaggccac gggccagggt gacgcgccgt ggccgcccaa
     4201 ctacgccaaa caggccggtg agccgccgcg ggtccagcca tcgcgggcac gccgcccgaa
     4261 gagcgagtac gagccgggcc gcggggtcga aggcggtccc ccacccgagg tcgcggccga
     4321 gcgggcggcc gcggtggcag ccggcgatcc gaacgccggc ttgccgaccg agtgggaggg
     4381 gtccgcgccg cgcggagcgc ccggtggctc gcgcccgaca ccgaccggac gccgcaggag
     4441 ctcgatcccg gtcatcgaga tcagccgggc agcgtcgaag aaggaagccc tcgagggcct
     4501 cgatcgctgg aaggcgcgcc atccggaggc ggccgctgcc ctcgagcccg ccgacatcct
     4561 gaccgacggc atgcgcggcc gctcctcgct ctggtaccgc gtccgggtca acctgatcca
     4621 cgtccccgaa gccgatcgcc cggcccagga ggccctcgac ccggactacg acccgtgggc
     4681 cggctacgag tggccggatc gttccgggca gctcgagcgg gcgccgcgca agcagtccac
     4741 gcggggctcg taagaggcgc ttcgatcggc catactcggt cgcctgaacc ggggccagcg
     4801 tcctcaccga ggtgtgatcg tggccagtac tcgcgtcgag tcctccgtca tcgctgtctc
     4861 ctggattcca tccgaggcga tcgagggccc gtcgaaggtc ccgttcgagc tcggcgtgac
     4921 gcactacgac cagccgcccc ctgaccacct cgacgatctg gaggacctcc gccggaccga
     4981 tcgcttccgg gaagccaacg agctccgcgc attcatcgag gtcgaggatg gacggatcgt
     5041 ggggcatggc catctcgggc aggggcacat cggcgcgacg acggttcgag tcgggccggc
     5101 ggccatccgc tttcctgccg tgcacctccc ggacatccgg cccgagccgg tcgtggggtc
     5161 cacgtcggtt cgattcgtcc agacggtcgg cggccggatg ggcctgccga cgccacggcc
     5221 ggtcccgcac aagccgttcg tccagttctg gccgtcgatc gcgtggacga cgcttgcgct
     5281 gaccatcaac gccgatggct cgtcgagcca cgagctcatc ggggcgagcc ccttcccgcg
     5341 tcactgggtg tacgaccacg agggcaacct ggtccagaag tctggcgtca tcgacttcaa
     5401 caagtggttc aacgattcgt tcggcgatcg gactccgtgg ggcgaggccg attctccggc
     5461 cgtcgtcgcc agcgtcgaat ccgcgctcga gcggcgactg tcgacgacga tcatgcggag
     5521 cggcgccaag ccgaagatcc ggacgctgtc ggcaggcgag acgctcatcg agcagggcgc
     5581 cgcgggcacc cagatgtacg tgatcctcga cgggatgttc gtcgtcgagg tggaggggaa
     5641 tgaggtcgcc gagatcgggc cgggagcggt cgtcggagag cgcgcggcgc tcgagggcgg
     5701 gacgcgcacg gccacgctcc gggcgcggac gcgcgcgagg gttgccgagg cgacgtccga
     5761 cgagctcgag ctcgaggagc tcggtgccct cgcgacgacc catcacgccg agggagatcg
     5821 acccgaggcg acctcggaac agccatgagc cacgaccttc gctgacctga cgcgcgcgct
     5881 gaccctggac gagttcgcga ccgaggccgc ggtgccggcg gacctggtcg atcgcctcgt
     5941 cgccgtcggt gcgatcacca ccctcgagga cggccgcgac gacagccggg acgagccggt
     6001 cgcctcgacc gcgcaggcac cgccgacttc gcgccgatcg gccgggtcga gctgaagggc
     6061 ttcccgatgg cggtcgtgct ctggcgcgcc gcacggcggg cggctgaggc acgagcaagg
     6121 ccgacggccg acggccgacg gccgacggct gaaatgggtg cgttgcgtgg cacgcatcca
     6181 ccgagcaaac gatccggcaa cgggagccga tgccgagccg aaatcggtca ccggatgcaa
     6241 cggctcgttg ccagcccggg gtccgtggac gcagggacgt cgattccttg catccgatga
     6301 aacgcggcgc ggcaatcgcg gggcgcggtc ctcaacgtgt gcgtgggacg caacgaccgg
     6361 agg
//
LOCUS       DATAIW010000422         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_191424, whole genome shotgun sequence.
ACCESSION   DATAIW010000422 DATAIW010000000
VERSION     DATAIW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191424"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VHM48_12710"
     CDS             <1..707
                     /locus_tag="VHM48_12710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2756322.1"
                     /translation="ADWAAADLRYEREAGWDGPTTPWNVTIDGLTLELRPTEAGQIGL
                     FPEQVAFWPWLRTALAGRTGPSVLHLFAHTGATTLALATAGAAVTHVDASRPAVAWAR
                     RNAELSDLADRPIRWIVDDALDFTRREARRGRRYDGFVLDPPSFGHGPRGTRWELADT
                     LPDLLDACAAIATDDAFVLLTAHTTGFQADDLGDALVDAFEPGPGGDVTTESIELVAR
                     SGAVLRLGVAARMIRG"
     gene            704..1522
                     /locus_tag="VHM48_12715"
     CDS             704..1522
                     /locus_tag="VHM48_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2756323.1"
                     /translation="MIQPPITSLTNPRVKAAVRLRDRREREATGLTIVDGAREILRAL
                     DAGVRVESAFLAEDLLRTPDAHAAADRLRHRPTTLAASPAVLAKVAFGERSDGIVAIV
                     ATPRIGLADLVLADEPLIVVIEGVEKPGNLGAVLRTADGAGAQAVIAADPRTDPFNPN
                     AIRASLGTIFAIPVVSATSRETIDWLAERGIRPVATIVDAPTDYAAADLRGPLAIVLG
                     SEAAGLSAEWHDPRVTPIAIPMHGIADSLNVSIAAAIVLYEAVRQRTVSTDRGH"
     gene            1528..2901
                     /locus_tag="VHM48_12720"
     CDS             1528..2901
                     /locus_tag="VHM48_12720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2756324.1"
                     /translation="MQTFDFVIIGAGPAGEAAANEARARGASVAIIDRRWFGGSCPFI
                     GCLPSKSLLHGAAEHAANPATYSWTRASAARDYMVNRAADAAEPDDSSHVRRLEDVGA
                     VCFRGNARIVGRDRVEIEHDGRTHEIVGKNVMVAVGSTSKIPPLPGLDGIRIWTNREA
                     TLARELPKSLVVLGGGPTGCELAQVYARFGVPVTVVQSGSRLMPTEHPRNSETVAALL
                     RRDGVDVRLGVRATGARAGAGTDGADVIDLDNGSTAEGHAILLAVGRTFPLDDLGLEH
                     YGLDTSGRTAFPRDGRLRVAEGLWIIGDPAGPELHTHQGHYQGEVAIRMALGLDVRPD
                     YRALPRATYTDPEAASVGLTLDQAKAAGVDAVEYVADFASSSKGYAVEATIGHVTVTF
                     DRATRQMVGAAMACPDASAAIHECVLAIRARVPIEVLADTIHAFPSTSRIFNGLFAEA
                     AKDLAPT"
     gene            2932..3765
                     /locus_tag="VHM48_12725"
     CDS             2932..3765
                     /locus_tag="VHM48_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX2756325.1"
                     /translation="MAEPPDAPILEFDPAREAVIEPTVAVTRFDVPSHAVLCFFADAI
                     PPFVKERGGREIGSVGSEMGSHPVFEVDVDGRRVAVAQGGVGAPLAAGWLEELIALGC
                     RAFVAVGGAGVLVPDVALGHVIVPISAVRDEGTSYHYLSASREVGPTDDALAAILATL
                     NRHAIPHVEGKTWTTDGLYRETREKVRRRVAEGCLTVEMEAAAFFAVARFRGVSFGQM
                     FYAGDDLSGETWDSRGWVGHSAGRDLLLRLAVEAVLGIALPLPASSADGDRRRSGSPP
                     S"
     gene            complement(3812..>4287)
                     /locus_tag="VHM48_12730"
     CDS             complement(3812..>4287)
                     /locus_tag="VHM48_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2756326.1"
                     /translation="FARAGIAASIQPVHVRADAPSARLLWGDRAETRGYPMRSLLEAG
                     AVVAFGTDAPVEPIDPWPGLSMAVTRIDGSWPPGAEPFGPDERLTLAEALRAACVAPA
                     VTAAEPDRGRLTPGQRADLVVIPAEALVHPIEPGGALATARPRLVMIDGEAVFER"
BASE COUNT          566 a         1593 c         1531 g          597 t
ORIGIN      
        1 gcgccgactg ggcggctgcg gaccttcgct acgagcgaga ggccggctgg gatggcccga
       61 cgacgccgtg gaacgtcacg atcgacggcc tgacgctgga gctccggccg accgaggccg
      121 gccagatcgg gctgttcccg gagcaggtcg cgttctggcc gtggctccgg acggcccttg
      181 ccggtcggac cgggccgagc gtgcttcacc tgttcgccca caccggggcg acgaccctcg
      241 cgctggcgac cgccggcgcc gcggtgaccc acgttgacgc gtcgcggccc gccgtcgcgt
      301 gggcccgccg caatgccgag ctgtccgacc tggccgaccg gccgatccgc tggatcgtcg
      361 acgacgcgct ggatttcacc cggcgcgaag cgcgccgggg gcgacgctac gacgggttcg
      421 tcctcgaccc tccgagcttc gggcacggac cgcgcgggac ccgctgggag ctggccgaca
      481 ccctgccgga tctcctcgac gcctgcgccg ccatcgcgac ggacgacgcc ttcgtcctcc
      541 tgacggctca tacgaccggc ttccaggcgg acgatctcgg cgacgcgctc gtcgacgcct
      601 tcgagcccgg gccgggtggg gacgtgacca ccgagtcgat cgagctggtc gcgcggagcg
      661 gagccgtcct tcggctcggc gtcgcagccc gcatgatccg cggatgatcc agcccccgat
      721 cacgagcctg acgaacccgc gggtcaaggc cgccgtccgt ctccgcgacc ggcgcgagcg
      781 cgaggcgacc gggctgacca tcgtcgacgg cgcccgcgag atcctgcgtg ccctcgacgc
      841 cggcgtccgc gtcgagtccg cgttcctggc cgaggacctg ctgcggacgc ccgacgccca
      901 cgcggccgcc gaccggcttc gccatcgacc gaccacgctc gccgcaagcc cggccgtcct
      961 cgccaaggtc gcgttcggcg agcgctccga cgggatcgtg gcgatcgtgg cgacgccgcg
     1021 gatcgggctt gccgatctcg tccttgccga tgagccgctg atcgtcgtca tcgagggtgt
     1081 cgagaagccc ggcaacctcg gcgcggtcct gcgcacggcc gacggcgccg gggcgcaggc
     1141 ggtgatcgcg gccgatccgc gaacggatcc gttcaacccg aacgcgatcc gggcgtcgct
     1201 cggcacgatc ttcgccatcc cggtcgtctc ggccacgtcc cgcgagacca tcgactggct
     1261 cgccgaacgc ggcatccggc cggtcgcgac gatcgtcgac gcgccgaccg actacgccgc
     1321 cgcggacctg cgcgggccgc tggccatcgt cctcggcagc gaagccgccg ggctgtcggc
     1381 cgagtggcac gatccccgcg tcacgccgat cgcgatcccg atgcacggaa tcgccgacag
     1441 cctcaatgtc tcgatcgcgg cggcgatcgt cctgtacgag gcggttcgcc aacgcaccgt
     1501 ttcgaccgac cgaggacact gacgggcatg cagaccttcg acttcgtcat catcggtgcc
     1561 ggtccggccg gcgaagccgc cgccaacgag gcgcgcgccc gcggcgcctc ggtcgcgatc
     1621 atcgatcgcc gctggttcgg cggcagctgt ccgttcatcg gctgcctgcc atccaagtcg
     1681 ctcctccacg gggcggccga gcacgccgcg aatccggcga catactcgtg gactcgggcg
     1741 tccgctgcgc gggactacat ggtgaatcgc gccgccgacg ccgcggagcc cgacgactcc
     1801 tcccatgtcc ggcggctgga ggacgtcggc gcggtctgct tccgcggcaa cgcccggatc
     1861 gtcggccggg accgggtcga gatcgagcac gatggacgga cccacgagat cgtcgggaag
     1921 aacgtcatgg tcgcggtcgg ctcgacctcg aagatcccgc cgttgcccgg gctcgacggg
     1981 atccgaatct ggacgaaccg ggaggcgacc ctggcccgcg agctgccgaa gagcctcgtc
     2041 gtcctcggcg gtggcccgac cggctgcgag ctcgcccagg tctacgcccg gttcggcgtc
     2101 ccggtcacgg tcgtccagtc aggcagtcgg ctgatgccga ccgagcatcc ccggaactcc
     2161 gagacggtcg cggccctcct ccgtcgcgac ggcgtcgatg tccgcctcgg cgtccgggcg
     2221 acgggcgctc gggccggcgc cgggaccgac ggcgccgacg tcatcgatct cgacaacggc
     2281 tcgacggccg agggccacgc gatcctgctc gcggtcgggc ggaccttccc cctcgacgac
     2341 ctcggcctgg agcactacgg cctcgacacg agcggccgga cggcgttccc gcgtgatggt
     2401 cgtctgcgcg tcgctgaagg gctctggatc atcggcgacc cggccggtcc cgagctccac
     2461 acccaccagg gtcattacca gggcgaggtc gcgatccgga tggcgctggg cctcgacgtc
     2521 cggcccgact accgcgccct gccccgggcg acctacaccg atccggaggc ggcgtcggtc
     2581 gggctgacgc tcgaccaggc gaaggcggca ggcgtcgacg ccgtcgagta cgtcgcggac
     2641 ttcgcgtcgt cgtcgaaggg ctacgcggtc gaggccacga tcggccacgt cacggtcacg
     2701 ttcgaccggg cgacgcggca gatggtcggc gcggcgatgg cctgccccga cgcgtcagcg
     2761 gcgatccacg agtgcgtcct cgcgatcagg gcccgcgtcc cgatcgaggt cctcgccgac
     2821 acgatccacg cgttcccgtc gacctcgcgg atcttcaacg ggctgttcgc cgaggcggcg
     2881 aaggacctcg ctccaacctg agcgggctgc accttgggga gaacgccgtc gatggccgaa
     2941 ccgcctgacg caccgatcct cgagttcgat ccggcgcggg aagccgtcat cgagccgacg
     3001 gtcgccgtca cgcgattcga cgtgcccagc cacgcggtcc tgtgcttctt cgcggacgcg
     3061 atcccgccgt tcgtcaagga acgcggcggc cgcgagatcg gaagcgtcgg atccgagatg
     3121 ggctcgcacc cggtcttcga ggtggacgtc gacggccggc gcgtggccgt cgcccagggc
     3181 ggcgtcggcg cgcccctcgc cgcaggctgg ctcgaggagc tgatcgcgct cggctgccgg
     3241 gcgttcgtcg ccgtcggggg tgccggggtg ctcgtcccgg acgtcgcgct cggccacgtc
     3301 atcgttccga tatcggccgt gcgcgatgag ggcacgagct accactacct gtctgcgtcg
     3361 cgcgaggtcg ggccgacgga cgacgcgttg gccgcgatcc tcgccacgct gaaccgacac
     3421 gccatcccgc acgtcgaagg gaaaacctgg accaccgacg gcctgtatcg cgagacccgg
     3481 gagaaggtcc gtcggcgcgt ggccgagggg tgtctcacgg tcgagatgga ggcggccgcc
     3541 ttcttcgcgg tggcccggtt ccgcggggtg tccttcggcc agatgttcta cgccggcgac
     3601 gacctctcgg gtgagacgtg ggactctcgc ggctgggtgg gccattccgc cggacgcgat
     3661 ctgctcctgc gactcgcggt cgaggcggtc ctcgggatcg cgctgccctt gccggcctcg
     3721 tcggccgacg gcgacagacg ccgatccggg agcccgccgt cctgatcgcg cagcgagcgg
     3781 atgctggagg cgggatcttc gctcgcgtcg cctagcgttc gaacacggcc tcgccgtcga
     3841 tcatcacgag gcggggacgg gctgtggcca gcgcgccacc gggctcgatt gggtggacga
     3901 gcgcctcggc cgggatcacc accagatccg cccgctggcc cggggttagg cggccgcggt
     3961 cgggctcggc ggcggtgacg gccggcgcga cgcaggccgc ccggagcgcc tccgcgaggg
     4021 tcagccgctc gtccggaccg aacggttcgg cgccgggagg ccacgacccg tcgatccggg
     4081 tgaccgccat cgacagcccc ggccacggat cgatcggctc gaccggggcg tcggtcccga
     4141 acgccaccac cgctccggcc tcgagcagcg accgcatcgg gtagccgcgc gtctccgctc
     4201 gatcgcccca caggagccgt gcgctgggcg cgtcggcccg gacgtggacc ggctggatcg
     4261 aggcggcgat cccggcccgg gcaaatc
//
LOCUS       DATAIW010000423         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_251043, whole genome shotgun sequence.
ACCESSION   DATAIW010000423 DATAIW010000000
VERSION     DATAIW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251043"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..>3000
                     /locus_tag="VHM48_12735"
     CDS             199..>3000
                     /locus_tag="VHM48_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2756327.1"
                     /translation="MTTIVVVAIIAFVVLMLTFGIDLWTDAIWYRSVGFDQVFWTRLG
                     IQGGLFALGGVVALVILLGNLWLAGRLSPASKGGAVGGGTIRAWIDRLNEAAANADPG
                     RSERRQWDRWNRSNTGEPENVTPLELPDPVPLGRLVIIIIAALTALGVAGSVAGSWET
                     IALWQNRVPFDPSGQAPPDPIFHLDISFFLFDLPFLRLLQTLASGLLVASLAVAAGRY
                     LLSAMSGAAIFDTRVRVHLGVLAGLFLMTIALGYQLDKLELVHSDRGIATGVSFTDAN
                     AQFLAYNVLTGLSAIAAALLIGGAFARVLWPVGLTLAVWFIASIAIGRLYPAAIQYFT
                     VNPNQQAQETPYIANNIAMTRLAFGLGSWTDQPYAGNASVTAATIAADSDTFANARLW
                     DYRPLGDTLGQLQTVQRYYAFTDVDTDRYAIGDSVRQVMLSGRELALENNTEATGWVN
                     QHIVYTHGIGVAMVPVNEVASQGQPHLIISNLPPVSTAGAPPIVRPQIYFGERPSSGY
                     AVVAARQDEFDYPRGVGDTSGTSGGTNATTRWAGKTGISLSTTLDRLLWSARFRDFNL
                     LISDQVTADSQLLFHRSLGERLPMIAPFLRYDKDPYLVIDGSGHMKYIQDAFTTSDAF
                     PHAQSFDPAAELGGTGLGGDPFNYIRNSVKIVTDAYDGSMTFYVADDTDPIIRAWEGV
                     FPTLFHPISELPADLVPHLRVPEELFDVQTRMYGRYHVTDPSTFYTNNDLWTVPAPAS
                     SKQSLPNEAYYVMMRMPGRDKAEFLLLQPMIPKNRPNMIAWVAAQNDPGVRGKVTVFR
                     FPSESSIFGPAQVEAQIDSDPIISAQTTLWDQAGSTVVRGNLIVVPVGGSLIYLQPVY
                     LQSASSKFPAFQRIVVASPRNIVWGTTLQEALNLLLAKEAGGGPGPSPSPGPTPTPTP
                     GPSATPAPGGSLPPGATVQQ"
BASE COUNT          447 a         1135 c          960 g          458 t
ORIGIN      
        1 ggtgacggtg cggacggcga gccctccgac gatccctccg gcgagccgca cccgatcccg
       61 ctccgacgcg ccggccgccg tcgctcgagc cgcgatggcc gcccggatgg cgacggtgac
      121 cgccgtcctc cgccgccgcg cgggccgcgg accgccgtcg ggggcccccg cgacggcggg
      181 ttctcgatgc ggtcgcgggt gaccacgatc gtcgtcgtcg cgatcatcgc cttcgtcgtt
      241 ctgatgctca cgttcgggat cgacctgtgg accgacgcga tctggtaccg cagcgtcggc
      301 ttcgaccagg tgttctggac gcgtctcggg atccagggcg gcctgttcgc cctcggtggc
      361 gtcgtcgccc tggtcatcct gctcggcaat ctctggctgg ccggacggct cagcccggcg
      421 agcaagggcg gagcggtcgg cggcggaacg atccgcgcct ggatcgaccg gctcaacgaa
      481 gcggccgcga acgccgaccc cggccgctcc gaacgccgcc agtgggatcg ctggaaccgc
      541 tcgaacacgg gcgagcccga gaacgtcacg ccgcttgagt tgccggatcc cgtcccgctc
      601 ggccggctcg tcatcatcat catcgccgcg ctgacggcgc tgggcgtggc cggttcggtc
      661 gccggcagct gggagaccat cgccctctgg cagaaccggg tccccttcga cccgtcgggg
      721 caggcccccc ccgacccgat cttccacctc gacatctcgt tcttcctgtt cgacctgccg
      781 ttcctgcgcc tgctccagac gctcgcaagc ggcctgcttg tcgcgtcgct ggccgtggcc
      841 gcgggccgct acctgctgtc ggcgatgtcg ggtgcggcga tcttcgacac ccgcgtccgc
      901 gtccacctcg gggtcctcgc cggcctcttc ctgatgacga tcgccctcgg ctaccagctc
      961 gacaagctcg agctcgtcca cagcgaccgg gggatcgcga ccggggtcag cttcaccgac
     1021 gcgaacgcgc agttcctcgc ctacaacgtc ctgaccgggc tgtcagcgat cgcggccgcg
     1081 ctcctcatcg ggggcgcgtt cgcccgcgtc ctctggccgg tcgggctgac gctcgcggtc
     1141 tggttcatcg cctcgatcgc gatcggccgg ctctatccgg cggcgatcca gtacttcacc
     1201 gtcaatccga accagcaggc ccaggagacc ccgtacatcg cgaacaacat cgcgatgacg
     1261 aggttggcct tcggcctcgg gtcatggacg gaccagccgt atgcgggcaa cgcctcggtc
     1321 accgcggcga cgatcgccgc cgactccgac accttcgcga acgcgcgcct gtgggactac
     1381 cgcccgctgg gtgacacgct cggccagctg cagaccgtcc agcggtacta cgcgttcacg
     1441 gacgtcgaca ccgatcgcta cgcgatcggc gactcggtcc gccaggtcat gctgtccggc
     1501 cgcgagctcg ccctcgagaa caacaccgag gcgaccggct gggtcaacca gcacatcgtc
     1561 tacacccacg ggatcggcgt cgcgatggtg ccggtcaacg aggtcgcctc gcagggccag
     1621 ccgcacctga tcatctcgaa cctgccgccg gtgtcgaccg ctggcgctcc gccgatcgtc
     1681 aggccccaga tctacttcgg cgagcgaccg agcagcggct atgcggtcgt cgccgccagg
     1741 caggacgagt tcgactatcc gcgtggcgtg ggcgacacct cgggcaccag cggcggcacg
     1801 aacgcaacga cgcgctgggc gggcaagacc gggatctcgc tcagcacgac gctcgaccgg
     1861 ctcctgtggt cggcacggtt ccgcgacttc aacctcctga tcagcgacca ggtgaccgcc
     1921 gacagccagc tcctgttcca ccgctcgctc ggagagcggc tgccgatgat cgcgccgttc
     1981 ctgcgctacg acaaggaccc gtacctcgtc atcgacggca gcggccacat gaagtacatc
     2041 caggacgcct tcacgacgag cgacgcgttc ccgcatgccc agtcgttcga cccggccgcc
     2101 gagctgggcg ggaccggcct cggcggcgac ccgttcaact acatccggaa cagcgtcaag
     2161 atcgtgaccg acgcctacga cgggtcgatg accttctacg tcgccgacga caccgacccg
     2221 atcatccggg cctgggaagg cgtgtttccg accctgttcc acccgatctc ggagctcccc
     2281 gccgacctcg tcccgcacct gcgcgtgccc gaggaactgt tcgacgtcca gacccggatg
     2341 tacggccgct accacgtgac cgacccgtcg accttttaca cgaacaacga cctgtggacc
     2401 gtgccggcgc cggcgagcag caagcagagc ctcccgaacg aggcgtacta cgtgatgatg
     2461 cggatgcccg gccgcgacaa ggcggagttc ctgctcctcc agccgatgat cccgaagaac
     2521 cgtccgaaca tgatcgcgtg ggtcgccgcc cagaacgacc cgggcgtccg cgggaaggtc
     2581 accgtcttcc ggttcccgag cgagagctcg atcttcggcc cggcccaggt cgaggcgcag
     2641 atcgattcgg accccatcat cagcgctcag accacgctgt gggaccaggc cgggagcacc
     2701 gtcgtccgcg gcaacctgat cgtcgtcccc gtcggcggat cgctgatcta tctccagccg
     2761 gtctacctcc agtcggccag ctcgaagttc ccggcattcc agcggatcgt cgtcgcgtcc
     2821 ccgcggaaca tcgtctgggg aacgaccctc caggaggccc tcaacctgct gctcgccaag
     2881 gaggccggcg gcgggcccgg gccgtcgcca tcgcccgggc cgaccccgac cccgacgccg
     2941 ggaccgagcg cgactcccgc tccgggcggg tcgctgccgc cgggcgcgac ggtccagcag
//
LOCUS       DATAIW010000424         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_28955, whole genome shotgun sequence.
ACCESSION   DATAIW010000424 DATAIW010000000
VERSION     DATAIW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28955"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHM48_12740"
     CDS             complement(<1..197)
                     /locus_tag="VHM48_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756328.1"
                     /translation="MTVLDQISNLWSDLLGFISKLVIPDWAGLIALLPVLILIGIVGP
                     LLSLAVLAWLGYGITKPRVKV"
     gene            complement(207..818)
                     /locus_tag="VHM48_12745"
     CDS             complement(207..818)
                     /locus_tag="VHM48_12745"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2756329.1"
                     /translation="MTRLSPRNDPMTGTTRLLVDGTNLLHQLSSGPERQPPAALIGRL
                     RAAIPPQVAIELVFDGPAERGLRGERIAHGVSVRYGGRHTADTILVTLVEDVAMAAGA
                     PAGGGSLATESILVVTDDRALRFAIQHRGARTAGASWLVARMARSTLSSPSVGNKRPP
                     VPPRLRQPTPGSVEEGEQDGPGWSPGRGATRKTGNGKRAPKHG"
     gene            1018..>1789
                     /locus_tag="VHM48_12750"
     CDS             1018..>1789
                     /locus_tag="VHM48_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX2756330.1"
                     /translation="MTAPGLLLLVDLDGVLYRGRDPVSGVAAVLAARAERGDDVVYVT
                     NNSMWYREEYVERIAGMGAPVTADRIVSSPRATALYLRDHEPTIRHVLTVGASGMDRE
                     LEDAGFRVTRAGDVGELIRGGGVEGTDGGNGWEAAGRPDAVVVGLDPKIDYLRLTVAN
                     DCVRAGARFIATNRDPVYPTERAVRPGAGSIVAAVEAASGVTPISIGKPEPYLLEEAA
                     RVVGRVAGEAIMIGDGLDTDIAAARAVGARSILMLTGVT"
BASE COUNT          252 a          623 c          685 g          229 t
ORIGIN      
        1 tggaccttga cccgcggctt cgtgatcccg tagccgagcc aggccaggac cgcgaggctc
       61 agcagcggcc cgacgatccc gatcaggatg aggaccggca gcagcgcgat cagtccggcc
      121 cagtcgggga tgaccagctt cgagatgaag ccgaggaggt ccgaccacag gttcgagatc
      181 tggtcgagga cggtcaccgg cgcattctag ccgtgtttcg gggcgcgctt cccgttgccg
      241 gtcttgcgcg tggcgccgcg gccgggcgac cagccgggac cgtcctgctc gccttcctcc
      301 acggagcccg gcgtcggttg ccggagccgc ggcgggacgg gtgggcgctt gttcccgacc
      361 gacggcgagg acagcgtcga gcgggccatc cgggcgacga gccacgaggc cccggccgtc
      421 cgggcgccgc gatgctggat cgcgaagcgg agggctcgat cgtcggtgac gacgaggatc
      481 gattcggtcg ccagcgagcc gcccccggcc ggcgccccgg cggccatcgc gacatcctcg
      541 acgagggtga cgaggatcgt gtcggccgta tgccggccgc cgtagcggac cgagacgccg
      601 tgcgcgatcc gctcgccgcg cagaccgcgc tcggcgggac cgtcgaagac gagctcgatg
      661 gcgacctggg gcgggatcgc cgcacgaagg cgaccgatca gcgcggccgg cggctggcgc
      721 tccgggcccg acgagagctg gtggagcagg ttcgtgccat cgacgagcag gcgggtcgtg
      781 ccggtcatcg gatcgttgcg cggcgagagg cgggtcaccg gccaagcgta gccgctcgcg
      841 cggcggcggc tcggccgcgc tcggcccggc ccgcgcacgc gccggaacgt cgctccgctg
      901 ggcgttcgaa tagccgaatg attcgaacct cgcaatattc ggctacacga aatggcagtg
      961 cagtcgaatg tcggcgagcc gggccggtga gacgggaccg ggtagcattc gccgctgatg
     1021 actgcccccg gactgctcct cctcgtcgac ctcgacggcg tcctgtaccg cggccgggat
     1081 cccgtgtccg gggtcgccgc cgtgctcgcc gcgcgggcgg agcggggcga cgacgtcgtc
     1141 tacgtcacga acaactcgat gtggtaccgc gaggaatacg tcgagcggat cgccgggatg
     1201 ggcgccccgg tgacggccga ccggatcgtc tcgtcgccgc gcgcgaccgc gctctacctg
     1261 cgcgatcacg agccgacgat ccggcacgtc ctgacggtcg gcgcgagcgg gatggaccgc
     1321 gagctcgagg acgccgggtt ccgggtgacc cgcgccggcg acgtcggcga gctgatccgc
     1381 ggcggcggcg tcgagggcac cgacggcggg aacggctggg aggccgccgg ccggcccgac
     1441 gcggtcgtcg tcgggctcga ccccaagatc gactacctcc gcctgaccgt cgcgaacgac
     1501 tgcgtccggg ccggcgcccg gttcatcgcc accaaccgcg accccgtcta tccgaccgag
     1561 cgggcggtcc ggcccggagc cggttcgatc gtcgccgcgg tcgaggccgc cagcggggtc
     1621 acgccgatct cgatcggcaa gcccgagccg tacctgctcg aagaggcggc gcgagtggtc
     1681 gggcgcgtgg cgggcgaggc gatcatgatc ggcgacgggc tcgacaccga catcgccgcg
     1741 gcccgggcgg tcggggcgcg aagcatcctg atgctgaccg gggtgacga
//
LOCUS       DATAIW010000425         5693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_178608, whole genome shotgun sequence.
ACCESSION   DATAIW010000425 DATAIW010000000
VERSION     DATAIW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178608"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHM48_12755"
     CDS             <1..202
                     /locus_tag="VHM48_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756331.1"
                     /translation="ETVHDGDQVDVVARLTSRTFGGYESLQLDIRDVASSGLHDAPAQ
                     VDIPAVGSEPAVGGGLTLPVAS"
     gene            199..1449
                     /locus_tag="VHM48_12760"
     CDS             199..1449
                     /locus_tag="VHM48_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756332.1"
                     /translation="MTRSGAPARARTPRAPRDPYGIGPVSGFVGPVLAMIALVVVAAL
                     TLSLMNGDIPFKTGTPGGGGDGGGDGPTPSPAPSNVVITPPSAVFPGSITYAKAGNIW
                     IQSGKDAHQVTNDGDDSMPTFSADGQWIYFIRVERTGGKFPTGGFQNPSYYDLNTPVL
                     FRIKPDGTGAKRLLTGRYTQGSYTWFYWLSQPVPSPNGKTIAVISDGPNPLQNDIVLH
                     TFNIASKKLTSLNLPESLHLGHQDPAWRPDGRYLLYVKNGREATRGAPQIYRYELATK
                     KTKALTGPGYLAPAYSPDGAWIAATKTDAFGTNIAILDQAGKEVLRVTDDDHSFSPIW
                     SPAGNAVAFLHLEGTIVDLRMATLDRSTGNWTVAATVDLTKVSGLDGASRPSWFVPPS
                     ELPAPSGAPGPGSSPAASTGSTAP"
     gene            1481..2371
                     /gene="pyrF"
                     /locus_tag="VHM48_12765"
     CDS             1481..2371
                     /gene="pyrF"
                     /locus_tag="VHM48_12765"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2756333.1"
                     /translation="MTEVADASYLGRLAVRSAATGTVLCLGLDPDPDALPDGFSRDLR
                     GVEAFARLVLDVAGPFAAAVKPNLAFFEAFGSAGIAALERLRVAVPAGLPVVMDAKRG
                     DIGTTAARQAAALYDDLGADAITISPYLGEEAIAPLIARSDRLAYVLCRTSNPGAGEL
                     QGLIVAADPALGAPAEPLWARVARRVQAWGPGGTVGLVVGATAPTELAAIRRLAPGLA
                     FLVPGVGAQGGDEAAVLADGRATAAPAGARAGGGLLVNVSRGIAGAARANTGSAPAGD
                     PADAIAAAARDWAARLPVLP"
     gene            2442..2798
                     /locus_tag="VHM48_12770"
     CDS             2442..2798
                     /locus_tag="VHM48_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2756334.1"
                     /translation="MPLPSGFEWIVILVIALLILGPGKLPDVAASVGKSIREFRKASA
                     DVQEAVKVDTSPLPAGPTATAPAPVAAAPGPVAAVPATVAAAPAPVAAVPAPVAAEPV
                     QEPVRSDASGSTPETH"
     gene            2820..3599
                     /gene="tatC"
                     /locus_tag="VHM48_12775"
     CDS             2820..3599
                     /gene="tatC"
                     /locus_tag="VHM48_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2756335.1"
                     /translation="MTNADALREGDLPTGVPSPSSDPSAVTIEDDRVMTLVDHLGELR
                     TRLVRSLIAVGIGSIVGFFFSTPIIATLAAPAGGKLLNLGPGDAFAITLRVSLVTGVV
                     LAMPVLLHQGWAFVAPGLTPAERRTLRPWIPLALLFFVVGVTIAYFVLPFAMGFLLSF
                     GTGVFINQLAAGQYFDFVTTLFLAFGLLMEFPILLYALSRVGIVTSERLRASRRMIIL
                     GITIFATVATPGGDLVSPTVLGLTMYLLFELTLIAIRRSGR"
     gene            3599..4561
                     /gene="rapZ"
                     /locus_tag="VHM48_12780"
     CDS             3599..4561
                     /gene="rapZ"
                     /locus_tag="VHM48_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2756336.1"
                     /translation="MIPDRDPAAIVDPDSPVDAEVDADVVDVADAVEGQHVVLLSGLS
                     GAGKTAAAKLFEDLGYTVVDNLPGELLPELAELVADNPERFGKVAIVLDIRSGDAPMA
                     FGAMRGALEGRGIRPQVFFLEARDDVLIRRFSETRHRHPLADQRGIASSIVAERRLLD
                     PVRSESNVVIDTSDLPLRELRERIFTRLGEAADPNRLAIQLISFGFKYGVPLEADLVF
                     DVRFMQNPYYIDELRQLSGLTEEVRSFVLGQPVAQEFLGYLRDFLGFALPAYIGEGKT
                     RLTIALGCTGGYHRSIALSEALADWLRERGFGPIAIFHRELDRT"
     gene            4558..>5693
                     /locus_tag="VHM48_12785"
     CDS             4558..>5693
                     /locus_tag="VHM48_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX2756337.1"
                     /translation="MTDSGRRDPASSSPDDETGDRPGVRRRIAADRLAVTNLRRWLTP
                     GIGVKRWLLVTFAGLLVLALGAAHVIRQATRDVEPGGLAGTILDAVTFQFLPYPLRGL
                     IVGVVGLALLGFGAYRTIGALTAPFRPSQGGGRLVELIYQRKSLARGPRVVVIGGGTG
                     QSTILRGLKEHTSNLTAIVAVADDGGSSGVLREELGIPPVGDIRRCLAALADAENLVG
                     EVLEHRFAGSPTDIAPEAGGPLGGHPVGNILLAAMTQLEGGDFEEGVRRANRVLAVRG
                     QVLPAAATPLTLHARTRDGTVVDGQSLITKTTGIERAWVTPANVAACEDALAAIAEAD
                     LIVLGPGSLYTSLLPSLLLPQIRDAVLASPALRLYVCNVATQDG"
BASE COUNT          771 a         2128 c         1913 g          881 t
ORIGIN      
        1 cgagactgtc cacgacggcg atcaggtcga tgtcgtcgca cgcctgacca gccggacatt
       61 cggcgggtat gagtcgctcc agctcgacat ccgggacgtg gcgagcagcg gcctccacga
      121 cgcgccagcg caggtcgaca taccggcggt cggcagcgag ccggccgtcg gcgggggcct
      181 gacgctcccg gtcgcgtcgt gacccgcagc ggggctccgg cccgggcacg gactccccga
      241 gcgcctcgcg atccgtacgg aatcggcccg gtctcgggct tcgtcggccc ggtcctcgcg
      301 atgatcgcgc tcgtcgtcgt cgccgcgctc acgctcagcc tgatgaacgg agacatccca
      361 ttcaagaccg ggacgcccgg cggcggaggc gacggcggag gcgacggccc gacaccgagc
      421 ccggccccgt cgaatgtcgt gatcacgccg ccgtccgccg tcttcccggg ctcgatcacc
      481 tatgccaagg ccggcaacat ctggatccag agcggcaagg acgcccacca ggtgacgaac
      541 gacggcgacg attccatgcc caccttctcc gccgacggcc agtggatcta cttcatccgg
      601 gtcgagcgga ccggtggcaa gttcccgacc ggcggcttcc agaatccgtc gtactacgac
      661 ctgaacaccc cggtcctgtt ccggatcaag cccgatggga caggcgccaa gcggctgctc
      721 accggccgct acacccaggg cagctatacg tggttctact ggctgagtca gcccgtcccc
      781 agcccgaacg gaaagacgat cgcggtgatc tccgacggcc cgaacccact ccagaacgac
      841 atcgtcctcc acaccttcaa catcgcatcg aagaagctca cgtcactgaa cctgccggag
      901 tcgctccatc tcggccacca ggaccctgcc tggcggcccg acggccggta tctcctctac
      961 gtcaagaacg gacgcgaggc gacgcgaggg gcgccgcaga tctatcgcta tgagctggcc
     1021 acgaagaaga cgaaggcgct gaccggaccc ggctacctcg ctcctgccta ctcacccgac
     1081 ggcgcgtgga tcgcggcgac gaagaccgac gccttcggga ccaacatcgc gatcctcgac
     1141 caggcgggca aggaggtcct ccgggtcacc gacgacgacc attccttctc gccgatctgg
     1201 tcgccggccg gcaacgccgt cgcgttcctc cacctcgagg gcacgatcgt cgatctgcga
     1261 atggccacgc tcgaccgttc gaccggaaac tggacggtgg ccgcaacggt cgatctcacc
     1321 aaggtgtccg ggctcgacgg cgcgtcgcgg ccgagctggt tcgtcccgcc gtccgagctg
     1381 ccggcgccga gcggcgcgcc cggtcccggc agctcgccgg ccgcatcgac cgggtcgacc
     1441 gccccgtgag gccgcgcccg gacctcgacc ggatcccgcg atgaccgagg tcgcggacgc
     1501 gtcatatctc ggccgcctcg ccgtccgatc ggcggcgacc ggcaccgtcc tctgtctcgg
     1561 tctcgacccg gatcccgacg ccctgcccga cgggttcagc cgcgacctgc gcggggtgga
     1621 ggcgttcgcc cgcctcgtcc tcgatgtcgc gggtccgttt gcggccgccg tgaagccgaa
     1681 cctcgcgttc ttcgaggcgt tcgggtcggc cgggatcgcg gccctcgagc ggctccgggt
     1741 ggccgtgccg gcgggcctgc cggtcgtcat ggatgccaag cgcggcgaca tcgggacgac
     1801 cgcggcgcgc caggcggcgg ccctgtacga cgacctcggc gcggacgcga tcacgatcag
     1861 tccgtatctc ggcgaggagg cgatcgcacc gctgatcgcg cggtcggatc gcctggccta
     1921 cgtgctgtgc cggacgtcca atccgggcgc cggcgagctc cagggcctga tcgtcgcggc
     1981 ggacccggcg ctcggcgccc cggccgagcc gctgtgggcg cgtgtcgccc gacgcgtcca
     2041 ggcctggggc cccggcggga ctgtcgggct cgtcgtcgga gccaccgctc ccaccgagct
     2101 cgccgcgatc cggcgcctgg cgcccggtct cgcgttcctc gttcccggcg tcggagccca
     2161 gggcggggac gaggcggccg tgctcgcgga cggccgggcg acggctgcgc cggccggggc
     2221 gcgagccggc ggtggcttgc tcgtcaacgt ctcgcggggg atcgcgggtg cggcccgtgc
     2281 gaacaccgga tcagcccccg cgggcgaccc ggccgatgcg atcgccgcgg cggcccgcga
     2341 ctgggcggct cgcctgcccg tgctacccta gccagcgcgc gaccggacgt ccgcggtcac
     2401 caacccgcgc caacgtgcgg tgattcgatg gagcacgcag catgcctctt ccgtccggct
     2461 tcgaatggat tgtcatcctc gtcatcgccc tcctgattct tggcccgggc aagctcccgg
     2521 acgtcgccgc gtcggtcggc aagagcatcc gtgaattccg gaaggcgtcg gccgatgtcc
     2581 aggaggcggt gaaggtcgac acctcgccgt tgcccgccgg gccgacagcg accgcgccag
     2641 cgcccgtggc ggcagcccct gggcccgttg cggcagtccc tgcgaccgtt gcggcagccc
     2701 ctgcgcccgt tgcggcagtc cctgcgccgg tcgccgcgga gccagtgcag gagccggtcc
     2761 gatcggacgc atctgggtcg acgcccgaga cccactgaat cgcggagcgc ccgcagccga
     2821 tgaccaacgc tgacgcgctg cgcgagggcg atctcccgac cggggtgccg tccccgtcat
     2881 ccgacccgtc cgccgtcacg atcgaagacg atcgggtcat gaccctcgtg gaccacctgg
     2941 gcgagcttcg aacccgcctc gtccgatcgc tgattgccgt cggcatcggc agcatcgtcg
     3001 gcttcttctt ctcgacaccg atcatcgcga ccctcgcggc gcccgccggg ggcaagctcc
     3061 tcaatctcgg cccgggcgac gccttcgcga tcacgctccg ggtctccctc gtgaccgggg
     3121 tcgtcctcgc gatgccggtc ctcctccatc agggctgggc gttcgtcgcc cctggtctga
     3181 cccccgccga gcgccgcacg ctccggccgt ggattcctct tgcgctcctg tttttcgtgg
     3241 tcggtgtcac gatcgcgtac ttcgtcctgc cgttcgcgat ggggttcctc ctgagcttcg
     3301 gaaccggcgt gttcatcaac cagctcgcgg cgggccagta cttcgacttc gtcacaaccc
     3361 tgttcctggc gttcggcctg ctgatggagt tcccgatcct cctgtacgcg ctgtcgcgcg
     3421 tcgggatcgt gacctcggag cggctccgcg cgtcgcgccg gatgatcatc ctcgggatca
     3481 cgatcttcgc caccgtcgcg acgccgggcg gcgatctcgt cagcccgacg gtcctcggcc
     3541 tcacgatgta cctcctgttc gagctgaccc tgatcgcgat ccgcagaagc ggccgctgat
     3601 gatcccggat cgggatccgg ccgcgatcgt ggatcctgac tcgcccgtcg acgcggaggt
     3661 cgacgcagac gtcgtcgatg tcgccgacgc ggtggagggc cagcacgtcg tcctgctcag
     3721 cgggctgtcg ggcgccggca agaccgcggc ggccaagctg ttcgaagacc tcggctacac
     3781 ggtcgtcgac aacctgcccg gcgagctgct cccggagctg gccgagctcg tggcggacaa
     3841 tccagaacgg ttcggcaagg tcgcgatcgt gctcgatatc cggtcgggcg acgcgccgat
     3901 ggcattcggt gcgatgcgcg gcgctctgga gggtcgcggg atccgccccc aggtgttctt
     3961 cctcgaggct cgcgacgacg tcctgatccg gcgcttctcc gagacccgcc accgccaccc
     4021 gctggccgac cagcgcggca tcgccagctc gatcgtggcc gagcgccgcc tcctcgatcc
     4081 ggtccgcagc gaatcgaacg tggtcatcga cacgagcgac ctgccgttgc gcgagctgcg
     4141 cgaacggatc ttcacccggc tcggcgaggc ggccgacccg aaccgactcg cgatccagct
     4201 gatcagcttc gggttcaagt acggcgtgcc cctcgaggcc gacctcgtgt tcgacgtccg
     4261 gttcatgcag aacccctact acatcgacga gctccgccag ctctccggtc tgaccgagga
     4321 ggttcgctcg ttcgtcctcg gtcagccggt cgcccaggag ttcctcggct acctgcggga
     4381 cttcctcggc ttcgccctgc cggcgtacat cggtgagggc aagacgcgcc tgacgatcgc
     4441 cctcggttgc accggcggct atcaccgttc gatcgccctg tccgaggcac tggccgactg
     4501 gctgcgcgaa cggggcttcg gaccgatcgc gatcttccac cgcgagctgg accggacgtg
     4561 accgactccg ggcggcgcga cccggcgagc tcgagcccgg acgacgagac cggcgaccgg
     4621 ccgggggtca ggcggcgtat cgccgccgac cggctcgcgg tgacgaacct ccgccgctgg
     4681 ttgacacccg ggatcggcgt gaagcgctgg ctcctcgtga cgttcgccgg cctgctcgtc
     4741 ctggccctcg gcgcggccca cgtcatccgc caggcgacgc gtgatgtcga gcccgggggg
     4801 cttgccggca cgatcctcga cgcggtcaca ttccagttcc tgccgtaccc gctccgcggt
     4861 ctgatcgtcg gtgtcgtggg gctcgccctg ctcgggttcg gcgcctaccg cacgatcggc
     4921 gcgctcacgg cgcccttccg gccgtcgcag gggggcggtc gcctcgtcga gctgatctac
     4981 cagcgcaagt cgctggcgcg cggcccgcgg gtggtcgtga tcggcggcgg caccggccag
     5041 tcgacgatcc tgcgcgggct caaggagcac acgagcaacc tgaccgcgat cgtcgccgtc
     5101 gccgacgacg gcggctcgtc cggagtcctg cgcgaggagc tcgggatccc gccggtcggc
     5161 gacatccggc gctgcctcgc cgcgctggcc gacgcggaga acctcgtcgg cgaggtcctc
     5221 gagcatcgtt tcgccggctc accgaccgac attgcgcccg aggccggtgg acccctcggc
     5281 gggcatccgg tcggcaatat cctgctggcc gcgatgaccc agctcgaggg cggtgatttc
     5341 gaggaagggg tccgccgagc gaaccgcgta cttgccgttc gcggccaggt tctgcccgcg
     5401 gcggcgaccc cgttgaccct tcacgcccgg acccgcgacg gcacggtcgt cgacggccag
     5461 tcgctcatca cgaaaacgac cgggatcgag cgggcatggg tgaccccggc caacgtcgcg
     5521 gcctgcgagg atgcgctcgc cgcgatcgcc gaagcggacc tgatcgtcct cggtccgggg
     5581 tccctgtaca cgagcctcct gccgagcctg ctcctgcccc agatccgcga cgccgtgctc
     5641 gcctcgccgg cgctccggct ctacgtctgc aacgtcgcca cgcaggacgg cga
//
LOCUS       DATAIW010000426         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_131852, whole genome shotgun sequence.
ACCESSION   DATAIW010000426 DATAIW010000000
VERSION     DATAIW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131852"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHM48_12790"
     CDS             <1..327
                     /locus_tag="VHM48_12790"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HEX2756338.1"
                     /translation="GGYWHPEKPWIDAFRQRLVADPAELHGLIDAPAFQAAFGGLSRD
                     GHALQRVPAGFAADHPEAEILKLKDVIFGKRLSDEEAYSADLPETIADTFAAGVPLLR
                     YLAALG"
     gene            complement(384..1421)
                     /locus_tag="VHM48_12795"
     CDS             complement(384..1421)
                     /locus_tag="VHM48_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756339.1"
                     /translation="MAAPRAAATDAGIRRFPTAAPLPAVRGLILRHLKAPDDLSAMNA
                     IANAIRDAEGANFWTTDEQFAMFYANPPGSDPATDVAVAEIDGRIIGYGRAAWHAELD
                     GTRIYEVIPFVDPTVVGHAVFMAMVDALETRLRVIAAGHPVGEKLFETFGGDLSPERD
                     TLLAAGEYAAVRFYYAMVRPSVDDLPDAPLPAGLQIREVRPEDLPTIWAADQEAFRDH
                     WGFAPSTEQEYEQFLTDPVQSDTTLWRVAWDGDEVAGQVRSFINAEENARHGRLRGYT
                     EHISVRRPWRRRGLARALIAASFPLLRARGMTEAALGVDTENPSGALRVYEGCGFRPV
                     TRSATYRKPFA"
     gene            complement(1421..2401)
                     /gene="deoC"
                     /locus_tag="VHM48_12800"
     CDS             complement(1421..2401)
                     /gene="deoC"
                     /locus_tag="VHM48_12800"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2756340.1"
                     /translation="MATIHRADTLARERAHELVRSWDGRPGALGSLLGRVSSVDQVGV
                     EERVDALKKRSIKKQSKLWALDLAIRMIDLTTLEGKDTPGKIRALCAKGMHPQPGDPT
                     IPSVAAICVYPAMVADAKEALTGSTVKVASVATGFPSGQTFRDIKLAEVRAAHDAGAD
                     EIDMVIDRGAFLSGDYLTVFEEIVDVKDASGDAHLKVILETGELETYDNVRRASVLAM
                     AAGADFIKTSTGKVVPAATLPVTLVMLEAIRDFERQTGRQVGMKPAGGIRVAKEAIQY
                     LVVLYETLGPRWMSPDWFRFGASSLLNDVLMQIEKERTGRYQGPDHFTID"
     gene            2513..3013
                     /locus_tag="VHM48_12805"
     CDS             2513..3013
                     /locus_tag="VHM48_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX2756341.1"
                     /translation="MATRSVHRLAVRGAIGAILALVATAGASAALAADHGVDIAGFAF
                     SPDSITIAVGDTVTWTNGDGVSHTATANDSSFDTGTIAAGSSRSATFSTAGTFSYHCR
                     IHPAMTATIVVAGSTTPPPTDTLDPAAASSNGGAPWALLALAALGGLVLGRRRFARPV
                     EAPAAD"
BASE COUNT          419 a         1080 c         1080 g          470 t
ORIGIN      
        1 ggcgggtact ggcatcccga gaagccgtgg atcgacgcct tccggcagcg tctcgtcgcc
       61 gatcccgccg agctccacgg gctgatcgac gcgccggcgt tccaggcggc gttcggtggg
      121 ttgagccgcg acggccacgc cctccagcgg gtcccggcag ggttcgccgc ggaccatccg
      181 gaggcggaga tcctcaagct gaaggacgtg atcttcggca agcgcctgtc ggacgaggag
      241 gcctattcgg cggacctgcc ggaaacgatt gccgacacct tcgcggccgg cgtcccgctc
      301 ctccgctatc tggccgcgct cggctgatcg ctcgttcggg cgccgggcga tcgaaccgac
      361 ggcgcggagc gacgcgcgcg aggtcaggcg aagggcttcc gataggtcgc ggaccgggtg
      421 accgggcgga agccgcagcc ctcgtagacg cggagcgcgc ccgacgggtt ctcggtgtcg
      481 acgccgagcg ccgcctcggt catgccccgc gcccggagca gcgggaagct ggccgcgatc
      541 agcgctcggg caagaccccg acggcgccac ggccggcgga cgctgatgtg ctcggtgtac
      601 ccgcgcagcc ggccgtgccg cgcgttctcc tcggcgttga tgaagctccg gacctgtccg
      661 gcgacctcgt ccccgtccca ggcgacccgc cacagggtcg tgtcggactg gaccggatcg
      721 gtcaggaact gctcgtattc ctgttccgtc gacggcgcga atccccagtg atccctgaag
      781 gcctcctggt cggccgccca gatggtgggc aggtcctcgg gtcggacctc gcggatctgg
      841 agtcccgccg ggagcggcgc gtcgggcaga tcgtcgacgc tcggacgcac catggcgtag
      901 tagaagcgga ctgccgcgta ctcgcccgcc gcgagcagcg tgtcgcgctc cggcgagagg
      961 tctccgccga atgtctcgaa gagcttctcc ccgacgggat gcccggcggc gatcacccgc
     1021 aggcgggtct cgagcgcgtc gaccatcgcc atgaacaccg cgtggcccac cacggtcgga
     1081 tccacgaatg ggatgacctc gtagatccgc gtgccgtcga gctccgcgtg ccacgccgcg
     1141 cggccgtagc cgatgatccg accgtcgatc tcggccacgg cgacgtccgt cgccggatcg
     1201 gagcccggtg ggttggcata gaacatcgcg aactgctcgt ccgtcgtcca gaagttcgct
     1261 ccctcggcat cgcggatcgc gttcgcgatc gcgttcatcg ccgacaggtc gtcgggtgcc
     1321 ttcaggtggc ggaggatcag gccccggacc gcgggcagcg gagccgcggt cgggaaccgc
     1381 cggatcccgg catcggttgc ggcagcccgc ggcgcggcca tcagtcgatc gtgaagtggt
     1441 ccggaccctg gtagcggccc gttcgttcct tctcgatctg catcaggacg tcgttgagca
     1501 gcgacgaggc gccgaagcgg aaccagtccg ggctcatcca gcgcggtccg agcgtctcgt
     1561 acaggacgac gaggtactgg atcgcctcct tggcgacgcg gatcccgccg gccggcttca
     1621 tcccgacctg gcgcccggtc tggcgctcga agtcgcggat cgcctcgagc atgacgaggg
     1681 tcaccggcag cgtcgccgcc ggcacgacct tgcccgtcga ggtcttgatg aagtcggcgc
     1741 cggcggccat cgccaggacg ctcgcccgcc ggacgttgtc gtaggtctcg agctcgccag
     1801 tctcgaggat caccttgaga tgggcgtcgc cgctggcgtc cttgacgtcg acgatctcct
     1861 cgaagacggt caggtagtcg ccgctcagga acgcgccgcg gtcgatgacc atgtcgatct
     1921 catccgcgcc ggcatcgtgg gcggcccgca cctcggcgag cttgatgtcg cggaacgtct
     1981 ggcccgacgg gaagccggtc gcgaccgagg cgaccttgac ggtcgagccc gtgagcgcct
     2041 ccttcgcgtc ggccaccatg gccgggtaga cgcagatcgc cgcgaccgac gggatcgtcg
     2101 ggtcgcccgg ctgagggtgc atccccttgg cgcagagtgc ccggatcttg cccggggtgt
     2161 ccttcccctc gagggtcgtc aggtcgatca tcctgatcgc gagatcgagc gcccagagct
     2221 tcgactgctt cttgatcgag cgcttcttca gcgcgtcgac ccgctcctcg acgccgacct
     2281 ggtcgaccga gctgacccgc ccgagcaggc tgccgagggc gcccgggcgg ccgtcccatg
     2341 agcggacgag ctcgtgggcg cgctcccggg cgagggtgtc ggcgcggtgg atcgtggcca
     2401 tgggacggtg aggatacacc ggccctcgac cccgttcgga gacggtcaat ccggtggggg
     2461 tgcgtcagcg tagccacccc tgacaccgcc ggggttgcag gagggtagac gcatggcgac
     2521 tcgttcggtc catcgactcg cggtccgagg cgccatcgga gcgatcctcg ccctcgtcgc
     2581 caccgccggc gcatccgcgg ccctcgcggc cgatcacggc gtcgacatcg ccggcttcgc
     2641 cttctcgccg gattcgatca cgattgccgt cggtgacacg gtcacgtgga cgaacgggga
     2701 cggcgtgagc cataccgcga ccgccaacga ctcctcgttc gataccggca cgatcgctgc
     2761 cgggtcgtcc aggtcggcga cgttctcgac ggcgggcacg ttcagctacc actgcaggat
     2821 ccacccggcg atgaccgcga cgatcgtggt cgcgggctcg accacgccac cgccgacgga
     2881 cacgctcgat ccggccgccg cgtcatccaa cggcggtgca ccgtgggcgc tcctcgctct
     2941 tgcggcgctc ggcgggctcg tcctcggtcg gcgacggttc gcgcgtcccg tcgaggcgcc
     3001 ggccgccgac tgacggcgct gccgcggcgg acgaggggcg ggccagggg
//
LOCUS       DATAIW010000427        13902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_281166, whole genome shotgun sequence.
ACCESSION   DATAIW010000427 DATAIW010000000
VERSION     DATAIW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281166"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VHM48_12810"
     CDS             complement(<1..1169)
                     /locus_tag="VHM48_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2756342.1"
                     /translation="MTGTAILAILLLLTGTFLDVPVAIVLSIVVLLLEIVRSIWGRFG
                     LERVTYRRTLERDRITWGEEIPTSIEIWNRKRLPLAWLRAEDEASPGVVVRERSLVVG
                     GHGARVLRNAWTLAPFERVTRQFHVTAERRGVYEIGPVGLTVGDLFAREAATGQHPGV
                     DRFLVRPRTVPTTALHRRDTWGGQDRARAGLSEDPSRFAGIREYAPGDPIRRIHSRAS
                     ARLGRPVVKRFEPSRDREVLLALDVQTADGPAWLTTGDAEEVESLFVVAASIARSLAL
                     ERAAFGLAAAGYHGAESRFAHVAVSEAPGQLERVFDLLARLSSHPSAPFDRLLGMILR
                     VIRPGTTVVVVTARDATQYLAWLRRIERAGCRVAIVACGPDGIADAARARRAGLVA"
     gene            complement(1173..2195)
                     /locus_tag="VHM48_12815"
     CDS             complement(1173..2195)
                     /locus_tag="VHM48_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2756343.1"
                     /translation="MEPASAAAPASDAPSPTDGATVAEHPARRLWEDLRTAVGQAVVG
                     AEAPLRLIITALLAGGHVLVEDVPGTGKTLLARAVARALTLDSNRVQGTPDLLPVDVT
                     GSSLYEGGTLRFAPGPVFTNILLVDEINRATPRTQAALLEAMQERQVSIEGTTRPLPD
                     PFIVLATQNPIEFEGTFALPQAQLDRFLLRVTIGYPDEAAERAIAHRYQTSAEPLDAI
                     EPVLDGPRLLDLRDQVRRVRVADEVEAYAVALVRATRVHADLQLGASPRAAVALYRAA
                     QAAAFLDGRRFVLPDDVQRIARAVLAHRLVVDLDQSLRGASVDNALSAIISTVPVPPI
                     TAGQEP"
     gene            complement(2899..5757)
                     /locus_tag="VHM48_12820"
     CDS             complement(2899..5757)
                     /locus_tag="VHM48_12820"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2756344.1"
                     /translation="MTHSDPFGARASLGAGLPDIYRLAAIADRIDLERAPVTLKILLE
                     NVLRHAGSGIVEAADVETLASWRAGVAAEAEVPFMPARVLLQDFTGVPAVVDLAVMRD
                     AMAELGGDPALVNPLVPADLVIDHSVQVDRFGTPGSFAFNVEREYERNGERYQLLRWA
                     QTAFRDLRVVPPGTGIVHQVNLEFLASVVTDAADPSGGPGRIAYPDTLVGTDSHTTMI
                     NALGVLGYGVGGIEAEAVLLGQPLYQPMPRIVGVRLHGELPQGSTATDLVLVVTQLLR
                     AHGVVGAFVEFAGDGLAGLALADRATISNMSPEFGATSTIFPIDDETLAYLRLTGRST
                     ERLSLVEAYAREQGLWRQPGPGPDFDEALELDLATVDPSVAGPRRPQDRVPLTALRDN
                     FRSNFPNGLVALDEAAVEAREPVGGEVEASSAESFPASDPPSFAAGDARGEEPPAVAE
                     PVSGPAVDDHHYRPVTIEVDGRQASIRTGSVAIAAITSCTNTSNPTVMVGAGLLARNA
                     VALGLSVGPTVKTSLAPGSKAVTGYLEAAGLMAPLEKLGFSLAGYGCTTCIGNSGPLD
                     DPIAKAIEDNDLVVAAVLSGNRNFEGRIHPLVRASYLASPPLVVAFALAGRVDIDLTI
                     EPLGRGSDGRPVMLSDIWPSPDGIRRVIGESIDPELFRRTYAVVFDGDERWQALPIPD
                     GDRYDWDPRSTYIAKPPFFDGLTADPAPVRDIDGARVLALLGDSVTTDHISPAGSIAA
                     WSPAGDWLQERNVGPLEFNSYGARRGHHEVMMRGTFGNIRLRNALVPGKEGPWTVHLP
                     DGEEQFIYDAAMRYRREETPLLVIAGREYGSGSSRDWAAKGTMLLGVRAVLAESYERI
                     HRSNLVGMGVLPLQFQPGDGASSLGLTGRETYTIHGLADDLSPRKRLTVVARADAADG
                     GGERRFEAIARLDGPIDVGYYQQGGILPAVLRRIAAGG"
     gene            5886..6977
                     /locus_tag="VHM48_12825"
     CDS             5886..6977
                     /locus_tag="VHM48_12825"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX2756345.1"
                     /translation="MKAWGDPDDDVVERAREETMRAIDEAGIDLDPMPPAKLAGQAHT
                     GDVAHTHDHAADGALPHSHPHTQSRPGEPPVIGIVGAGAVGTALGVALDRAGWRIHAV
                     ASRNAERRDRFRSLVPGVRAFADATPLVEEVELIVLAVPDDAVVAVAGSLHMYGGQAM
                     IHTSGLLGVEALAAALAAGTQVGSFHPLVAFADLERAVAALHGATIAIEGDDQLLDLL
                     ARMAEAVGGSPVRLAPGAKPAYHAAAVLAAGGFDALLDAIAELGRVAGLDEAGSLAIY
                     GPLIEQTLGNARTLGIAAALTGPMVRGDRGTVVSHLAALRAHAPGAVPVYRALAEREI
                     AIALERRALTPEAATDLRATLAEPLATTV"
     gene            6997..7317
                     /locus_tag="VHM48_12830"
     CDS             6997..7317
                     /locus_tag="VHM48_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756346.1"
                     /translation="MEHSIAARYAARQTARFVSPSAKARERRLGLRGAGSFRPAPARL
                     TVLHQPGGSVLARLRGDWRAIRPAHPRRTDGRPRPTVSMSWTGSSPAGSTRRSSAATA
                     PWRA"
     gene            7305..7808
                     /locus_tag="VHM48_12835"
     CDS             7305..7808
                     /locus_tag="VHM48_12835"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2756347.1"
                     /translation="MARLRTGTATSAGGIVVRFDDGIPSLVVGARRRDPDIFTWTLPK
                     GTPNAGETREQTAVREVAEETGLEVRITDVLDSIEYWFIQRGTRIHKTVHYFLMEPIG
                     GDLDRHDHEFDKVRWVRFDEAGELLTFETERALVARAAEIVDPPASRPSGLPTVHPAA
                     GAPLGGV"
     gene            7836..8951
                     /gene="gcvT"
                     /locus_tag="VHM48_12840"
     CDS             7836..8951
                     /gene="gcvT"
                     /locus_tag="VHM48_12840"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX2756348.1"
                     /translation="MTDTLEPTVHLTPLNDRHVALGARLIDFAGWLMPVQYAGIIEEH
                     RAVRERAGLFDLSHMGELFVEGPEAGAALARALVTNPPALADGRAHYSMIVAADGGII
                     DDLIVYRLGPDRFLVVANAGNARTVSDTLAERLTGFKAVLDDRSLALALVAIQGPRSV
                     DIVRPLTDVDLDGLRYYAVAEGMFAGIPALVARTGYTGEDGFEVFVDTDRALELWDAM
                     MAAGTDQGLAPIGLGARDTLRLEAGMPLYGNELDRSTNPFEANLGRVVKLDKPGDFIG
                     RAALERVAREGVARRLVGLIMRGRGIARHGYPVLSGDRRTGVVTSGTQSPSLGEAIAM
                     AYVATADAEPGTMVDVEIRGQRVPAEVVALPFYQRAR"
     gene            9071..9451
                     /gene="gcvH"
                     /locus_tag="VHM48_12845"
     CDS             9071..9451
                     /gene="gcvH"
                     /locus_tag="VHM48_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2756349.1"
                     /translation="MVPSDLRYTKDHEWVRVDGDEATVGITEYAASQLGDIVFVELPD
                     TGRSVAQFAAFGVVESVKAVSDLFAPLSGEVTAANAALAGSPELVNGDPYGEGWMVRL
                     RLAAPDEVEDLLDQAAYDALIAAG"
     gene            9458..10882
                     /gene="gcvPA"
                     /locus_tag="VHM48_12850"
     CDS             9458..10882
                     /gene="gcvPA"
                     /locus_tag="VHM48_12850"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX2756350.1"
                     /translation="MPYGPHTAGDRERMLAALGIDSVDALFADIPESLRASRLDLPGP
                     EPELELAARLQGLAGRNRTDLASFLGAGVYRHWTPPNVDQLLLRGEWYTAYTPYQPEI
                     SQGTLQSIYEYESLLAELVDLDVVSASHYDGAAATAEAGLMTCRATHRDRVLVSKAVH
                     PHYRQALLAYFGHGLELDDIPLVADGDAAGTTDLAALERLLADGERPVAGVIAGQPNF
                     FGLLEPMAEIGRLAHAAGALFVSVIEPVSLAVLAPPGAYGADIAAGEGQPLGIAPQYG
                     GPYLGILASTDALVRQIPGRLVGMTTDVKGRRAYVMTMRAREQDIRRDKAASNICTNQ
                     ALLALAASIYLATIGPHGLRDVAAQGAARAGRLETALAAVGAARVHPGPYLNEFAVRV
                     PDARTVHRRLIERGVLAGLVLGDIIPDEPSLADALLVCATEVTTDAEIAQFASALATE
                     LAGSAMAAGGPATTSASVTVEAAR"
     gene            10879..12375
                     /gene="gcvPB"
                     /locus_tag="VHM48_12855"
     CDS             10879..12375
                     /gene="gcvPB"
                     /locus_tag="VHM48_12855"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX2756351.1"
                     /translation="MTVVGEQLQPSLFERSRPGRGGDKIPHPPKDALDRIPADARRAT
                     PPALPELNEPEVVRHYVNLSQLNYSIDTGFYPLGSCTMKFNPKLNEWAARLPGFASLH
                     PLAPDEVAQGTLQLLFELEEWLAEISGMTAVTLQPAAGAQGELTGILMIRAYHRSRGD
                     TERDEVLVPDSSHGTNPATASMAGFRTITIPSADGGVDNEAFKAALGPRTAAVMITNP
                     STLGLFEPRIGLLLEEVHAAGALAYMDGANLNAILGRFKPGEAGFDVMHFNVHKTFST
                     PHGGGGPGAGPVGVREALLPFLPSPRVLRSDDGSFRLERAGERPESIGRLRSFVGNTG
                     VLVRAYAYLRAHGGTGLRQVSDDAVLAANYLRKRVGEAYDIPFDRVCKHEFVASAAGI
                     KKRTGVRTLDIAKRLIDYGYHPPTIYFPLTVEEGMLIEPTETESVETLDAFADALIAI
                     ARECETDPELVKGAPHDAPVGRLDEATAARQPNLRWRPMAGAEMPCPD"
     gene            12484..13092
                     /locus_tag="VHM48_12860"
     CDS             12484..13092
                     /locus_tag="VHM48_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756352.1"
                     /translation="MDDASSDVELLRRVQADDLDAFEAFFARYRGPVFRTAYGLTGDR
                     QAAEEILQDAFVRAFQRRQTLRLDVSPLPWLHRVTLNLCYSRLSRRRINTGPIDTAAE
                     SRVPDDRSLPSDDAERAELRSIVRAGIAALPEKHRSVVVLYYLDRLSLQETAQVLGIQ
                     LGTVKSRLHYALRGLRDHLEHDRRFGGAYGPMPVDDPGVGAS"
     gene            13089..13667
                     /locus_tag="VHM48_12865"
     CDS             13089..13667
                     /locus_tag="VHM48_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756353.1"
                     /translation="MIELFRTCRRHRAALVDFVDHGLIGPPTAAALAHLDRCDACTEM
                     LDSTVLAIAALRRLGEEAARVEPRTDAWPRLRARLSTRRPSRLSIVSPLAGVFTSVAL
                     AALLVAPIHLGGGSETERIGGAAGASDRTSSPTTAAYSSSARRGTDGSNSPDPSKSRA
                     GIQPKLIYPDGGRPLEKEVPSARSIVRPAEPR"
BASE COUNT         1997 a         5038 c         4950 g         1917 t
ORIGIN      
        1 gccaccaggc cggcccgtct cgcccgcgcc gcatcggcga tcccgtccgg tccacaggcg
       61 acgatcgcca cgcgacaccc ggcgcgctcg atccgccgaa gccaggcgag gtactgcgtc
      121 gcgtcgcggg ccgtcacgac gaccaccgtc gtcccgggcc ggatgacgcg gaggatcatc
      181 ccgagcagcc ggtcgaacgg cgcggacggg tgcgacgata gccgggcgag caggtcgaag
      241 acccgctcga gctggcccgg cgcctcagac accgcgacgt gcgcgaaccg gctctcggcc
      301 ccgtgatagc cggcggccgc caggccgaac gccgcccgct cgagcgcgag ggagcgggcg
      361 atcgaggcgg ccacgacgaa cagggactcg acctcctcgg cgtcgccggt cgtcagccac
      421 gccgggccgt ccgcggtctg gacatcgagg gcgaggagga cctcgcggtc gcgcgacggc
      481 tcgaaccgct tgacgaccgg gcggccgagg cgggcgctcg cgcgcgagtg gatgcgtcgg
      541 atcgggtcgc ccggggcgta ttcgcggatg ccggcgaacc gcgacggatc ctcgctcagg
      601 cccgcccgcg cccggtcctg gccgccccag gtgtcgcgcc ggtggagggc ggtcgtcggg
      661 acggttcgcg gtcggacgag gaaccggtcg acccccggat gctggccggt tgccgcctcc
      721 cgggcgaaca ggtcgccgac ggtcagtccg acgggcccga tctcgtagac gccgcgccgc
      781 tcggcggtga cgtggaactg gcgggtcacc cgctcgaatg gggccagggt ccaggcgttg
      841 cggaggaccc gcgcgccgtg cccgccgacg acgagcgacc gctcccggac gacgaccccg
      901 gggctggcct catcctcggc ccgcagccag gccagcggca ggcgcttgcg gttccagatc
      961 tcgatcgagg tcgggatctc ctcgccccag gtgatccggt cgcgctcgag ggtccgtcga
     1021 taggtcaccc gctcgagccc gaagcggccc cagatcgacc gaacgatctc gaggaggaga
     1081 acgacaatcg acagcacgat cgcgaccggg acgtcgagga aggtgcccgt gaggaggagc
     1141 aggatcgcca ggatcgccgt ccccgtcatc ggctagggct cctggccggc cgtgatcggc
     1201 ggcacgggca cggtgctgat gatcgcgctg agggcgttgt cgaccgaggc gccccgcagg
     1261 ctctggtcga gatcgacgac gagccgatgg gcgaggaccg cccgggcgat ccgctggacg
     1321 tcgtcgggca ggacgaaccg tcgaccgtcg aggaacgcgg cggcctgcgc ggcacggtac
     1381 aaggcgacgg ccgcgcgcgg actggccccg agctggaggt cggcatgaac ccgggtcgcc
     1441 cggacgaggg cgacggcgta ggcctcgacc tcatcggcca cccggacccg gcggacctgg
     1501 tcgcggagat cgagcaggcg cggcccgtcg aggaccggct cgatcgcgtc gagtggctcg
     1561 gccgacgtct ggtagcgatg ggcgatggcg cgctcggcgg cctcgtcggg atagccgatc
     1621 gtgacccgca gcaggaaccg gtcgagctgg gcctggggca gggcgaacgt gccctcgaac
     1681 tcgatcgggt tctgggtcgc gaggacgatg aagggatcgg gcaggggccg ggtcgtgccc
     1741 tcgatcgaga cctgccgctc ctgcatcgcc tcgaggaggg cggcctgcgt ccgcggcgtg
     1801 gcccgattga tctcgtcgac gaggaggatg ttcgtgaaga cggggccggg agcgaagcgg
     1861 agcgtcccac cctcgtacag gctcgagccg gtgacgtcga ccggcaggag atccggtgtg
     1921 ccctggaccc ggttcgagtc cagcgtcaga gcccgggcga ccgcccgggc gaggagggtc
     1981 ttgccggtcc cgggcacgtc ctcgaccagg acgtggccac cggcgaggag cgcggtgatg
     2041 atcaggcgga gcggcgcctc ggcgccgacg acggcctgcc cgaccgccgt tcggaggtcc
     2101 tcccagagcc tccgcgcggg atgctcggcg acggtggctc cgtccgttgg cgatggggca
     2161 tcggacgctg gcgcagccgc cgacgcgggc tcgatgtcgg tgtccgtggc gggctcctcg
     2221 ggcggtcggg gctctcgaca gggatacgcg ccgggagcgc aggtcgctca gcctacccga
     2281 gttgcttcgg agcgatcccg tgcggccccc ttccgcgtcg tctcgcagcg attgccgcgc
     2341 cctcgtgtca gataccaccg cccgtggtga gggtcagagg aggcgcagtc atggccggtc
     2401 atgcacgatc ccccgtggtg cctgaccaca gcgaggctgg attcgaccgc cagatcgaga
     2461 tggagccgga tacgcaccac gccggatggt gaatggcatc catggcgccc aggtggcggg
     2521 ccggcgccag gtggcgggcc ggcgccaggt ggcgggcgcg gatcgcgcgc ggggatcgcc
     2581 ggtccacgac ccgtgtcacg gccgacggga gttctgcgtc acatgcctga cgctcggatc
     2641 ggcgaatgac cgaccggcgt cagaagaagg gatggaacct ccatgacccg ctggtggcaa
     2701 gagttcaccg gcctcctcgc acgtcggcgc cacgaccgga tcgtcgcccg ccggctgcgg
     2761 acctatgtcg ggagtccagc cggccgctga cccatcgcac tgcctgtccc gaagcccccg
     2821 gaatcccacc gggggcttcg tcacgtccgg gtccgtccgc tcgggcggag cggacggcca
     2881 gccgtcccgc ttcgacgctc agccgccggc ggcgatccgt cggagcaccg ccggcaggat
     2941 cccgccctgc tggtagtacc cgacgtcgat cgggccgtcg agccgggcga tcgcttcgaa
     3001 ccggcgctcc ccgccaccgt cggcggcgtc cgcccgggcg accaccgtca gccgcttccg
     3061 cggggagagg tcgtcagcca ggccgtggat cgtgtacgtc tcgcggcccg tgagtcccag
     3121 cgaggacgcg ccatcaccgg gctggaactg gagtggcagg acgcccatcc ccacgaggtt
     3181 cgagcggtgg atccgctcgt agctctcggc gaggaccgcc cggactccga ggagcatcgt
     3241 ccccttggcg gcccagtcgc gcgacgaccc ggagccgtac tcgcggccgg cgatcacgag
     3301 gagcggcgtc tcttcgcggc ggtagcgcat cgcggcgtcg tagatgaact gctcctcgcc
     3361 gtcgggcagg tggaccgtcc aggggccttc tttgccgggc accagggcgt tccgcagccg
     3421 gatgttgcca aacgtgccgc gcatcatcac ctcgtggtga ccgcgccggg cgccatacga
     3481 gttgaactcg agaggcccga cgttccgctc ctggagccag tcaccggccg gcgaccaggc
     3541 ggcgatcgac ccggcgggcg agatgtgatc ggtcgtgacc gagtcgccga gcagggcgag
     3601 gacgcgggcg ccgtcgatgt cgcggaccgg ggccgggtcg gcggtgagcc cgtcgaagaa
     3661 cggcggcttg gcgatgtagg tcgagcgcgg atcccagtcg tagcggtcgc catcggggat
     3721 cggcagggcc tgccatcgtt cgtcgccgtc gaagacaacc gcgtaggtcc ggcggaagag
     3781 ctccgggtcg atcgattcac cgatcacccg gcggatcccg tcgggcgatg gccagatgtc
     3841 ggagagcatc acgggacggc cgtcggagcc gcgcccgagc ggttcgatgg tcaggtcgat
     3901 gtcgacccgg ccggcgaggg cgaacgcgac gacgagggga ggggaggcga ggtacgacgc
     3961 ccggacgagg ggatggatcc ggccctcgaa gttgcggttg cccgacagga cggcggcgac
     4021 gacgaggtcg ttgtcctcga tcgccttcgc gatcggatcg tcgagcgggc cggagttgcc
     4081 gatgcaggtc gtgcaaccgt agccggcgag gctgaagccg agcttctcga gcggggccat
     4141 caggccggcg gcctcgagat agccggtcac ggccttcgag cccggggcga gggacgtctt
     4201 gacggtcggc ccgaccgaca gcccgagggc gaccgcgttc cgggcgagga ggccggcccc
     4261 gaccatgacg gtcgggttcg acgtgttcgt gcaggaggtg atcgcggcga tcgcgaccga
     4321 accggtccgg atcgacgcct gccggccgtc gacctcgatg gtgacgggtc ggtagtgatg
     4381 gtcgtcgacg gcgggcccgg acaccggctc cgcgacggcc ggtggctcct cgccgcgggc
     4441 atcgccggcc gcgaacgagg gcggatcgct ggccggaaac gactcggcgg atgacgcctc
     4501 gacctcaccc ccgaccggct cgcgggcctc gactgcggcc tcgtcgaggg cgacgagccc
     4561 gttcgggaag ttcgaccgga agttgtcgcg gagggcggtc aacgggacgc gatcctgggg
     4621 ccgacgtgga ccggccaccg atggatcgac ggtcgcgagg tcgagctcga gtgcctcgtc
     4681 gaagtcaggc cccggccccg gttgccgcca caggccctgc tccctggcat atgcctcgac
     4741 caggctgagc cgctcggtcg agcgaccggt caggcggagg taggccagcg tctcgtcgtc
     4801 gatcgggaag atcgtcgagg tcgccccgaa ctcggggctc atgttgctga tcgtcgcccg
     4861 gtcggcgagg gccagcccgg cgaggccgtc gccggcgaac tcgacgaagg cgccgacgac
     4921 gccgtgggcc cgcagcagct gggtcacgac gaggacgagg tcggtcgccg tggagccctg
     4981 cggcaactcg ccgtggagcc ggacgccgac gatccgcggc atcggctggt agagcggctg
     5041 gccgaggagg acggcctcgg cctcgatccc gccgacgccg tagccgagga caccgagcgc
     5101 gttgatcatc gtcgtgtgcg aatcggttcc gacgagggtg tcgggatagg cgatccggcc
     5161 cggcccgccg ctcggatccg ccgcgtcggt cacgacgctg gcgaggaact cgaggttgac
     5221 ctggtgaacg atgccggtgc cgggcggcac gacccgcagg tcgcggaacg ccgtctgcgc
     5281 ccagcgcagg agctggtagc gctcgccatt ccgctcgtac tcccgctcga cgttgaacgc
     5341 gaacgatccg ggcgtcccga accggtcgac ctggacggag tggtcgatga cgaggtcggc
     5401 cgggacgagc gggttgacga gggccgggtc gccgccgagc tcggccatcg cgtcgcgcat
     5461 cacggccagg tcgacgaccg ccggcacgcc ggtgaaatcc tggaggagca cccgtgccgg
     5521 catgaacggg acctccgcct cggcggcgac gccagctcgc cacgaggcga gggtctcgac
     5581 gtccgctgcc tcgacgatcc cgctgccggc gtggcggagg acgttctcga ggaggatctt
     5641 gagcgtgacc ggtgcccgtt cgaggtcgat ccgatcggcg atcgcggcga gccggtagat
     5701 gtcgggcagg cctgccccga ggctggcccg agcgccgaag ggatcggaat gggtcacgcg
     5761 tcctccgtcg tgatcggtcg gtacactcgc tcgatggaag agtaccgcgc atccggctcg
     5821 cgacccggac cgcgcccgcg cccgcgctcg acggcgccgt ccgcgcgcgc cctgcggcca
     5881 acctcatgaa ggcctggggc gatcccgacg acgacgttgt cgagcgcgct cgcgaggaga
     5941 cgatgcgggc catcgatgag gcggggatcg acctcgatcc gatgccaccg gcgaagctcg
     6001 ccggccaggc ccataccgga gacgtcgcgc atacccacga ccacgctgcc gacggcgccc
     6061 ttccgcacag ccacccgcat acccagagcc gtcccggtga gccgccggtg atcgggatcg
     6121 tcggggccgg cgcggtcggc accgccctcg gcgtcgcgct cgaccgggcc ggctggcgga
     6181 tccacgccgt cgcgagccgc aacgccgaac gccgcgaccg gttccggagc ctggttcccg
     6241 gggttcgcgc gttcgccgac gccacccccc tggtggagga ggtcgagctg atcgtgctcg
     6301 ccgtccccga tgacgccgtc gtggccgtcg ccggctccct ccacatgtat ggcggccagg
     6361 cgatgatcca cacgagcggc ctcctcggcg tggaagctct cgccgcggca ttggcggccg
     6421 gaacccaggt cgggtcgttc cacccgctgg tcgcgttcgc cgacctagag cgggcggtcg
     6481 ccgcgctcca cggcgcgacg atcgcgatcg agggcgacga ccagctgctc gatctcctcg
     6541 cccggatggc cgaggccgtc ggcggcagcc cggtccgcct cgcgccgggc gccaagcccg
     6601 cctaccacgc ggccgcggtc ctcgccgccg gtggcttcga cgcgttgctc gatgcgatcg
     6661 ccgagcttgg ccgggtcgcc gggctcgacg aggccggcag cctcgcgatc tatgggccgc
     6721 tgatcgagca gaccctgggc aacgcccgga cgctcgggat cgccgccgcc ctcactggac
     6781 cgatggtccg cggcgaccgc ggcacggtgg tcagccacct cgccgcgctc cgggcgcatg
     6841 cccccggtgc cgttccggtg tatcgcgccc tggccgagcg ggagatcgcg atcgctctcg
     6901 agcggcgcgc gctgacaccg gaagcggcga cggacctgcg agccacgctc gcggagccgc
     6961 ttgcaacgac tgtctgaggc agtaccattg ccgcccatgg agcacagcat cgccgcacgc
     7021 tacgcggccc gccagaccgc gcggttcgtc agcccgtcgg cgaaggcccg cgagcggcgg
     7081 ctgggtctcc gcggcgccgg ctccttccgc ccggcaccgg cccgcctcac ggtcctccac
     7141 cagcccggcg gcagtgtcct cgcccgcctc cgcggcgact ggcgagcgat ccggccggct
     7201 catccgcggc gaaccgacgg gcgaccccgg ccgactgtct cgatgagttg gaccggctcc
     7261 tcacctgccg gctcgacccg tcgatccagc gccgccacgg cgccgtggcg cgcctgagga
     7321 ccggcaccgc cacatcggcc ggtggcatcg tcgtccgctt cgacgacggg attccgtcgc
     7381 tcgtcgtcgg cgcccgccgg cgcgatccgg acatcttcac gtggaccttg ccgaagggga
     7441 cgccgaatgc cggtgagacc cgcgagcaga ccgccgtccg cgaagtggcg gaggagaccg
     7501 gcctcgaggt ccggatcacc gatgtcctgg actcgatcga gtattggttc atccagcgcg
     7561 ggacccggat ccacaagacc gtccactatt tcctgatgga gccgatcggc ggcgatctcg
     7621 atcgccatga ccacgagttc gacaaggtcc gctgggtccg cttcgacgag gccggcgagt
     7681 tgctgacgtt cgagaccgaa cgagcgctgg tcgctcgcgc ggcggagatc gtcgatccgc
     7741 ctgccagccg accctccggc ctgcccaccg tccacccggc cgcgggcgcg ccgctcggcg
     7801 gggtctgagc cgcagcgcgg cgcccgagga gcccgatgac cgacacgctg gagccgactg
     7861 tccacctgac gcccctcaac gaccgccatg tcgccctcgg cgcgaggctg atcgactttg
     7921 ccggctggct catgcccgtc cagtacgcgg ggatcatcga ggagcaccgg gccgtccgcg
     7981 aacgggctgg cctgttcgac ctgtcgcaca tgggcgagct gttcgtcgaa ggtcccgagg
     8041 cgggtgccgc cctcgccagg gccctcgtga cgaacccgcc tgcgctcgcc gacggccgtg
     8101 ctcactactc gatgatcgtc gccgcggacg gcgggatcat cgacgacctg atcgtctacc
     8161 ggctcggccc ggaccggttc ctcgtcgtcg ccaacgcggg caacgcgcga accgtgagcg
     8221 acaccctcgc ggagcgcctg accgggttca aggccgtcct cgacgaccgc tccctcgcgc
     8281 tggccctcgt cgccatccag ggcccgcgct cggtcgacat cgtccggccg ctgaccgacg
     8341 tcgacctcga tgggcttcgc tactatgcgg tcgccgaggg aatgttcgcc gggatcccgg
     8401 cgctcgtcgc acggaccggc tataccggcg aggacggatt cgaggtcttc gtcgacaccg
     8461 atcgggcgct ggagctgtgg gacgcgatga tggccgccgg caccgaccag ggcctggccc
     8521 cgatcggcct cggcgcccgg gacacgctcc gcctcgaggc cggcatgccg ctctacggca
     8581 acgagctcga tcgctcgacg aaccccttcg aggcgaacct cggacgggtg gtgaagctcg
     8641 acaagcccgg ggacttcatc ggtcgagccg cgctcgagag ggtcgctcgc gagggcgtcg
     8701 cccgacgcct cgtcggactg atcatgcgcg gccgggggat cgcccgccac ggctatccgg
     8761 tcctgtccgg tgaccgacgg acgggcgtcg tcacgagcgg cacccagtcc ccctcgctcg
     8821 gcgaggcgat cgcgatggcc tacgtcgcca cggccgacgc cgagccgggt acgatggtcg
     8881 atgtcgagat ccgcggtcag cgggtccccg ccgaggtcgt cgccctgccg ttctatcagc
     8941 gggcgcgctg agcggggacg ccgggccgtc gctcggcgcc gaacccgtcg ccaggccagc
     9001 cgtcgtccgg cgcccgataa cgcccggcga cgagcggtcc cggacgggcc gccaaggagg
     9061 agaagctcgg atggtccctt cggatctgcg ctacaccaag gatcatgagt gggtccgcgt
     9121 ggacggcgat gaggcgacgg tcggaatcac cgagtacgcc gccagccagc tcggcgatat
     9181 cgtcttcgtc gagctcccgg acaccggccg cagcgtcgcc cagttcgccg cgttcggggt
     9241 cgtcgagtcg gtcaaggcgg tcagcgacct gttcgctccg ctgtccggcg aggtgacggc
     9301 ggccaacgct gccctggccg gctcaccgga gctcgtcaac ggcgatccgt acggcgaggg
     9361 ctggatggtc cggctccggc ttgccgcccc ggacgaggtc gaggacctcc tcgaccaggc
     9421 cgcctacgac gcgctcatcg cggcgggctg accggcgatg ccctacggac cgcataccgc
     9481 gggcgaccgc gagcggatgc tcgcggccct cgggatcgac agcgtcgacg ccctcttcgc
     9541 cgacatcccc gagagcctcc gggcgagccg cctcgacctg ccgggcccgg aacccgagct
     9601 cgagctggcc gcccgcctcc agggcctggc cgggcgcaac cggaccgatc tcgcgtcctt
     9661 cctcggcgcc ggggtgtacc gccactggac cccgccgaac gtcgaccagc tcctcctccg
     9721 cggtgagtgg tacacggcct acacgccgta ccagcccgag atcagccagg gcacgctcca
     9781 gagcatctac gagtacgagt cgctcctcgc cgagctcgtc gacctcgacg tcgtgtcggc
     9841 atcgcactac gacggcgccg cggccacggc cgaagcgggc ctgatgacct gccgggcgac
     9901 ccaccgcgac cgcgtcctcg tgtcgaaggc cgtccatccc cactaccgcc aggcgttgct
     9961 cgcgtatttc ggccacggcc tggaactgga cgacatcccg ctcgtcgctg acggtgacgc
    10021 cgccggcacg accgacctgg cggcgctcga acgcctcctg gccgacggcg aacggccggt
    10081 cgccggcgtg atcgccggtc agccgaactt cttcggcctg ctcgagccga tggccgagat
    10141 cggacgcctc gcccacgcgg cgggggcgtt gttcgtcagc gtcatcgagc ccgtctctct
    10201 ggctgtcctg gctccccccg gcgcctacgg ggcggacatc gccgccggcg aaggccagcc
    10261 gctcggcatc gccccgcagt acggggggcc gtacctcggg atcctcgcct cgaccgacgc
    10321 cctcgtccgc cagatccccg gccggctcgt cgggatgacg accgacgtca agggccggcg
    10381 ggcctacgtc atgacgatgc gcgcgcgcga gcaggacatc cggcgcgaca aggccgccag
    10441 caacatctgc accaaccagg ccctcctggc tctcgcggca agcatctatc tcgccacgat
    10501 cgggccgcac ggcctgcgtg acgtcgccgc ccagggtgcc gctcgtgccg gccgtctcga
    10561 gaccgccctg gccgccgtcg gggcggcgcg ggtccatccc gggccgtacc tcaacgagtt
    10621 cgccgtccgc gttccggacg cccggaccgt ccatcgccgg ctgatcgagc gcggcgtcct
    10681 cgccggcctc gtccttggcg acatcattcc cgacgagccg agcctcgccg acgccctcct
    10741 ggtctgcgcg acggaggtca cgaccgacgc ggagatcgcc cagttcgcct cggccctggc
    10801 gaccgagctg gcgggctccg ccatggccgc gggtgggccg gcgacgacga gcgcctccgt
    10861 caccgtggag gcagcgcgat gaccgtcgtc ggtgagcagc tccagccctc gctgttcgag
    10921 cgatcgcgac ccggccgcgg cggcgacaaa atcccccatc cgcccaagga cgccctcgac
    10981 cggatcccgg ccgacgcgag gcgggcgacc ccgccggcgc tgcccgagct caatgagccc
    11041 gaggtcgtcc gccactacgt caacctctcc cagctgaact actcgatcga cacgggcttc
    11101 tatccgctcg gctcgtgcac gatgaagttc aacccgaagc tcaacgaatg ggccgcccgc
    11161 ctgccgggct tcgcctctct ccacccgctc gcgccggacg aggtggccca gggcaccttg
    11221 cagctcctct tcgagctcga ggaatggctg gccgagatca gcgggatgac cgcggtcacc
    11281 ctgcagccgg cggccggcgc ccagggcgag ctgaccggga tcctgatgat ccgggcctat
    11341 caccgcagcc ggggcgacac cgagcgcgac gaggtcctcg ttcccgacag ctcgcacggg
    11401 acgaacccgg ccaccgcgtc gatggccggc ttccggacga tcacgatccc ctcggcggac
    11461 gggggcgtcg acaacgaggc gttcaaggcc gccctcggac cgcgaaccgc cgccgtcatg
    11521 atcacgaatc cgtcgacgct cgggctgttc gagccgcgga tcgggctgct cctcgaggag
    11581 gttcacgcgg ccggcgccct ggcctacatg gacggcgcca acctcaacgc catcctcggc
    11641 cggttcaagc ccggcgaggc cggcttcgac gtgatgcatt tcaacgtcca caagaccttc
    11701 tcgacaccgc acggtggggg agggccgggc gcgggaccgg tcggtgtccg tgaggcgctt
    11761 ctgccgttcc tgccgtcgcc ccgcgtcctc cgctccgatg acggctcgtt ccggctcgag
    11821 cgggcggggg agcgaccaga gtcgatcggg cggttgcgca gcttcgtcgg caacaccggt
    11881 gtgctcgttc gcgcgtatgc ctacctgcgg gctcacggcg gaacggggct ccgccaggtc
    11941 agcgacgatg ccgtcctcgc ggcgaactac ctccgcaagc gcgtgggcga ggcgtacgac
    12001 atcccgttcg atcgcgtctg caagcacgag ttcgtggcgt cggcggccgg gatcaagaag
    12061 cggaccggcg tccggaccct cgatatcgcc aagcggttga tcgactacgg ctaccacccg
    12121 ccgacgatct acttcccgct gacggtcgag gaggggatgc tgatcgagcc gaccgagacc
    12181 gagtcggtcg agacgctcga cgcgttcgcc gacgccctga tcgcgatcgc ccgcgagtgc
    12241 gagacggacc cggagctcgt gaagggcgca ccccacgacg cacccgtcgg ccggctcgat
    12301 gaggcgacgg ccgcccgcca gccgaatctc cgctggcggc cgatggccgg cgcggagatg
    12361 ccctgcccgg attgaacctt ttcggcggaa gcgtcgtatt gtctgatcaa ggggcggccc
    12421 aaggggccac atcgccagca cggagaacgg acgacgggag actctactcg ggcctcgacg
    12481 tcgatggacg acgcctcaag cgacgtcgag ctgctgcgtc gtgtccaggc tgacgacctc
    12541 gacgcgttcg aagcgttctt tgcacggtac cgcggacccg ttttccgaac ggcgtatggc
    12601 ctgacgggcg atcggcaggc agccgaggaa atcctccagg acgccttcgt ccgcgcgttc
    12661 cagcgccgac agaccctccg gctcgacgtc tcgcccctgc cgtggcttca tcgggtcacc
    12721 ctgaatctct gttactcgcg cctgtcgcga cggcggatca acacggggcc gatcgatacc
    12781 gccgccgaaa gcagggtccc ggacgaccga tccctgccgt ctgacgatgc cgaacgcgcc
    12841 gagctccgct cgatcgtccg ggcggggatc gccgccctgc cggagaagca ccggagcgtc
    12901 gtcgtcctct actacctgga ccgcctgtcc ctgcaggaaa ccgcccaggt gctcgggatc
    12961 cagcttggca cggtcaagtc ccggctccat tacgccttgc gcgggctgcg ggaccacctc
    13021 gagcacgacc gccggttcgg tggcgcctat gggccgatgc ccgtcgacga tccgggggtc
    13081 ggggcgtcgt gatcgagctg ttccggacct gtcggcggca ccgcgcggcc ctcgtcgact
    13141 tcgtcgacca cgggctgatc ggaccgccga cggccgccgc gctcgcccat ctcgacagat
    13201 gtgatgcgtg caccgagatg ctcgattcga ccgtcctcgc catcgccgct ctgcgacggc
    13261 tcggcgaaga ggcggcccgg gtcgaaccac ggaccgacgc gtggccgcgc ctgcgcgccc
    13321 ggctctccac ccgacgcccg agccgcctgt cgatcgtctc gcccctcgcc ggcgtattca
    13381 cgagcgtcgc cctcgcagcc ctgctcgtgg ccccgatcca cctcggtggc ggtagcgaga
    13441 ccgagcggat cggcggcgcg gcgggagcct ccgatcgaac cagctcgccg acgacagccg
    13501 cctacagcag ttcggcgcgg cgcggcactg acggatccaa ttcccccgac ccatcgaagt
    13561 cgcgagcggg aatccagccc aagctgatct acccggacgg tggccgtccg ctggagaagg
    13621 aggtgccgtc ggcccgttcg atcgtccggc cagccgaacc tcgctaggcg tcaggatcgg
    13681 caccattcat ggccgatttg acaccgctcg gggggccatc gcatagtacc ggccagtcca
    13741 gtgaggctgt tcaagaccgg ccaggactcg ttccgcgtgc accgtccatc gggattgcct
    13801 gcgcaattcc cggcggaccg ccagcggcgg gagtctggat ccggatccgg aacgcaccac
    13861 ggaaagcagg aggagagaat ctacatgcgg aatcgcttat tc
//
LOCUS       DATAIW010000428         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_191621, whole genome shotgun sequence.
ACCESSION   DATAIW010000428 DATAIW010000000
VERSION     DATAIW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191621"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VHM48_12870"
     CDS             complement(<1..1944)
                     /locus_tag="VHM48_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX2756354.1"
                     /translation="MPATPSWRNAFGVVALVVACTWLLATLSPVVASTPHRLAGPITD
                     DVSALTGDTASVQSSLDELQRATGAQLWVWYTDTLEGQDASAFAAATAKASDLGTKDL
                     LLVIALDDHEYGWWKGSGVAIGDAELDQVLSTDMEPMLQAADYPGAITATAAGLLAAL
                     TGGPTTAPETVVPGGGETPDVNPLPTPGSPASDGGSAIAAVILALIVVGLIGGVWWYV
                     SRARASGGTGGRAGVATNPDDPNGDLAAMQPKDLDELANRILVETDDSIRDSDQELGF
                     AQAQFGDEAATPFVAAIAAARGDLKGAFTLRQQLDDATPEDHATRRRMLTDLVIACRT
                     AQGRLHAELQHFEELRALEKEAPDILARLPAAADAIEARLPAAQATLTGLAEYADADW
                     QAVGANVDNARTRIAAVREAAGQGTKAVAAGTAGRAAQAARLGEDGLAQATAFLDAID
                     RLAAQLAEARSKVDAEIAAAQAEIAKAKAAATGAPPDVDATARLAEAEALLAEARTAL
                     DPPKPDVAGAYDKARRADDLADAIEQDIRNAAEQRARDAARLDAGLRTATVTVTRAAD
                     YIGGQRGGIGTEARTRLAEASRHLDQAVALRPTDAAGALAEADQATRLAAEAQRLAEA
                     DYGRWDDPFHGGPGPSGPGGGGGG"
     gene            complement(2177..4447)
                     /gene="aceA"
                     /locus_tag="VHM48_12875"
     CDS             complement(2177..4447)
                     /gene="aceA"
                     /locus_tag="VHM48_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HEX2756355.1"
                     /translation="MSPREAQLAAASEWFAQPRFDGIVRLHTAREVAAQQGTIQPDYT
                     VARTAAEAFHARLRELFSRGEQITTFGPYSPGQAVVMKRLGIEAIYLGGWATSAKGST
                     SEDPGPDLASYPLGQVPDEAAGLVRALLAADKNQHFARTRMDDAQRRATPAVDFRPFI
                     IADADTGHGGDAHVRNLIRRFVEIGVPGYHIEDQKPGAKKCGHQSGKVLVAQDEQNKR
                     LNAARLQLDVMRVPGIIVARTDAEAATFLDNRSDERDQPFILGATNLELPSYRIGFLA
                     IQKTLHDLGCADIRGHLLFAVPDDDMRNATSWLVEVGLMALVEETAETIRETGGGVPI
                     DDLLDAIDTRYAEIWQSEADLKTCGQAVADILELRISEGERFAMTVEEWQAFAAGASF
                     SEVRAKADALDIRLTWDAELPKTPDGYYQVRGGIDYAIAKSLAAAPFADLLWMETKTA
                     DLEDARRFAEAIHARYPDKMLAYNLSPSFNWDTTGLSDDEMRRFPEDLGKLGFVFNFI
                     TYGGHQIDGLAAEEFATALRDDGMLALARLQRKFRLLESPYRTPQTLVGGDRLDAALM
                     AVSGRTAATKAMGKGSTQHQHLVQTEVPTRLLEGWLATWATHWERPANLRVELRPHTA
                     GSDLLELRVVDDSDDPLANVIFASIVDRRGRSILSIRDQNTAAAVRRRRLMTLVQLFL
                     IHRYRALSLHYLTPTEDNRSQTQRMQDLGIFSIVRTEIGQIIVADVNRERIVELVRPE
                     GDALAELIGEAIPQHA"
     gene            4789..>5493
                     /locus_tag="VHM48_12880"
     CDS             4789..>5493
                     /locus_tag="VHM48_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2756356.1"
                     /translation="MLLAQIEGFVEIARVGTMRRAADALLISQPALTARLQALEEELA
                     APLFRRTHTGMVLTPAGRAFLPHAERAMEAIRSGGSLVRELEHGVVGELALAVAPAVS
                     AYVLPEILVRFTERHPDVRLRVRTGHSEEIVDLVVRGEVELGIVRKLRDGRVRSRALY
                     EDELVLVARPDHPFAAAGNVDVSEISHVQLILFDRTSSYYEVTSALFRAAGVVPRGIT
                     EVDNIEAAKRMVDRGLG"
BASE COUNT          710 a         1910 c         1972 g          901 t
ORIGIN      
        1 cccgccgccc ccgccgccgg gaccgctcgg acccggtccg ccgtggaagg ggtcatccca
       61 gcggccgtag tccgcttcgg cgagacgttg cgcctcggcc gcgagccgcg tggcctggtc
      121 ggcctcggcc agggcaccgg cggcgtccgt cgggcgaagg gcgacggcct ggtcgaggtg
      181 ccggctcgcc tcggcgaggc gcgttcgcgc ctccgtcccg atgccgccgc gttgtccgcc
      241 gatgtaatcc gcggcgcggg tcaccgtgac cgtggcggtt cgcagcccgg cgtcgagtcg
      301 ggcggcgtcg cgcgcccgct gctccgcggc gttgcggatg tcctgctcga tcgcatcggc
      361 caggtcgtcg gcacggcggg ccttgtcgta ggccccggcc acgtccggct tgggcgggtc
      421 gagcgccgtg cgcgcctcgg cgaggagcgc ttcggcctcc gcgagccttg cggtcgcgtc
      481 gacgtccggt ggggcgccgg tcgccgcggc cttcgccttc gcgatctccg cctgcgccgc
      541 ggcgatctcc gcgtcgacct tcgagcgcgc ctcggccagc tgcgcggcga ggcggtcgat
      601 ggcgtcgagg aacgccgtcg cctgggcgag cccgtcctcg ccgaggcggg ctgcctgggc
      661 ggccctcccc gccgttccgg ccgcgacggc cttcgtcccc tggcctgcgg cctcgcgcac
      721 cgcggcgatc ctcgtccggg cgttgtcgac gttcgcgccg acggcctgcc agtccgcgtc
      781 cgcgtattcc gcgaggccgg tcagggtcgc ctgcgcggcc gggagcctgg cctcgattgc
      841 gtcggcggcc gccggcagcc tggcgaggat gtcgggagct tccttctcga gggcccgcag
      901 ctcctcgaag tgctggagct cggcgtgcag ccggccctgc gccgttcgac aggcgatcac
      961 gagatccgtc agcatccgcc gccgcgtggc atggtcctcg ggcgtcgcgt cgtcgagctg
     1021 ctggcggagc gtgaatgcgc ccttgaggtc accccgcgcc gcagcgatcg cggccacgaa
     1081 tggggtcgcc gcctcgtcgc cgaactgcgc ctgcgcgaac ccgagctcct ggtcggagtc
     1141 gcggatcgag tcatcggtct cgacgaggat ccggttcgcg agctcgtcga ggtccttggg
     1201 ctgcatcgcc gcgagatcgc cgttcggatc gtcggggttc gttgcaacgc cggcccggcc
     1261 gccggtcccg ccggacgctc gcgcgcggga cacgtaccac cacaccccgc cgatcaggcc
     1321 gaccacgatg agggcgagga tgacggccgc gatcgccgag cctccgtcgg aagccggcga
     1381 accgggtgtg ggcagcggat tcacgtccgg cgtctcgcca ccgccgggca ccaccgtctc
     1441 gggcgcggtg gtcgggccgc cagtcagggc agccagcagc ccggcggcgg tcgccgtgat
     1501 cgcgcccggg tagtcggccg cttgaagcat cggctccatg tccgtcgaga ggacctggtc
     1561 gagctcggcg tccccgatcg cgacgccgct gcccttccac cagccgtact cgtggtcgtc
     1621 gagggcgatg acgagcagga ggtccttcgt cccgaggtcg ctcgccttgg ccgtcgccgc
     1681 tgcgaaggcg ctggcatcct ggccctcgag ggtgtccgtg taccagaccc agagctgggc
     1741 gccagtggct cgctggagct cgtcgaggct ggactgcacg gacgcggtgt cgccggtcag
     1801 ggcactgaca tcgtcggtga tcggtccggc aagacgatgc ggcgtcgacg cgacgacagg
     1861 ggagagggtc gccaacaacc aggtgcatgc gacgacgagg gcgacgacgc cgaacgcgtt
     1921 gcgccaggat ggggtcgccg gcatcgtgct ccttcaacgc gacggcgtct ggtcgagcgc
     1981 ggtcgcgtga ccccattgtc gctcgatcgg tcgcggcgcg agtgacgtcg tccgccgcct
     2041 gcggccgcgg ctcgcccgcc gatgtcgtcg gacttgcgcg tcccttggac gatctcccgg
     2101 ccgctcccgg ctcgccgccg gccgtcgagg ttcgtccgct cgtccaggcg ccttgcccgc
     2161 gagaccggcc cacgagctat gcgtgttgcg gaatcgcctc cccgatcagc tccgcgaggg
     2221 cgtccccctc gggccggacc aattcgacga tccgctcgcg attgacatcc gcgacgatga
     2281 tctggccgat ctcggtccgg acgatcgaga agatgcccag gtcctgcatc cgctgcgtct
     2341 gcgatcggtt gtcctcggtt ggcgtcaggt agtggaggga cagggcgcga tagcggtgga
     2401 tcaggaacag ctggacgagc gtcatcaggc ggcgcctgcg gactgctgcc gccgtgttct
     2461 gatcgcggat cgagaggatg ctccggcccc gccgatccac gatgctcgcg aagatcacgt
     2521 tggcgagggg gtcgtccgag tcgtcgacga cgcgcagctc gaggaggtcg gatccggccg
     2581 tgtgcggccg gagctccacg cggaggttcg ccggccgctc ccagtgggtc gcccaggtcg
     2641 cgagccagcc ctcgagcagc cgcgtcggca cctcggtctg gacgagatgc tgatgctggg
     2701 tcgagccctt gcccatggcc ttcgtcgccg cggtccgccc ggacaccgcc atcagggccg
     2761 cgtccagccg gtcgccgccg acgagggtct ggggcgtccg gtacggcgat tcgagcagcc
     2821 ggaacttccg ctggagacgg gccagggcga gcatcccgtc gtcgcgcagc gcggtcgcga
     2881 actcctcggc ggcgaggccg tcgatctggt ggccgccgta ggtgatgaag ttgaagacga
     2941 agccgagctt cccgaggtcc tcggggaatc gccgcatctc gtcgtcgctc agcccggtgg
     3001 tatcccagtt gaacgagggc gacaggttgt aggcgagcat cttgtccgga taccgggcat
     3061 ggatcgcctc cgcgaagcgc cgggcgtcct caagatcggc ggtcttggtc tccatccaca
     3121 ggaggtcggc gaacggcgcc gccgccagcg acttggcgat cgcataatcg atcccgcctc
     3181 ggacctggta gtagccgtcc ggggtcttcg gcagctccgc gtcccaggtc agccggatgt
     3241 cgagggcgtc cgccttggcg cgcacctcgg agaacgatgc gccggcggcg aatgcctgcc
     3301 actcctcgac ggtcatggcg aaccgctcgc cttcgctgat ccggagctcg aggatgtcgg
     3361 caacggcctg gccgcaggtc ttgaggtccg cttccgactg ccagatctcg gcataccggg
     3421 tgtcgatggc gtccagcagg tcgtcgatcg ggaccccgcc gccggtctcg cgaatcgtct
     3481 cggcggtctc ctcgacgagc gccatcagcc cgacctcgac gagccagctc gtggcgttcc
     3541 gcatgtcgtc gtccgggacc gcgaagagga ggtgaccgcg gatgtcggcg cacccgagat
     3601 cgtggagcgt cttctggatc gccaggaagc cgatccggta gctcggcagc tccaggttcg
     3661 tcgcgccgag gatgaacggc tgatcccgct cgtcgctgcg gttatccagg aacgtggccg
     3721 cctccgcgtc ggtccgagcg acgatgatgc ccggcacgcg catgacgtcc agctgcaggc
     3781 gcgccgcgtt gagccgcttg ttctgctcat cctgggcgac caggaccttg ccgctctggt
     3841 gaccacattt cttggcgccc ggcttctggt cctcgatgtg gtagcccggg acgccgatct
     3901 cgacgaatcg gcggatgaga ttccgaacgt gcgcgtcgcc gccgtggccg gtgtcggcgt
     3961 cggcaatgat gaatgggcgg aagtcgaccg ccggcgtggc ccgccgctgg gcgtcgtcca
     4021 tccgggtacg cgcgaaatgc tggttcttgt cggcggcgag gagcgcgcga accaatccgg
     4081 ccgcctcgtc cgggacctgg ccgagagggt agctcgcgag gtccggaccg gggtcctctg
     4141 atgtcgagcc tttcgcggac gtcgcccagc cgccgaggta gatggcttcg atccccagcc
     4201 gcttcatcac cacggcctgg ccgggtgaat acgggccgaa cgtcgtgatc tgctcgcccc
     4261 gcgagaacag ctcgcgaagt cgggcatgaa aggcttccgc cgccgtccgg gcgaccgtgt
     4321 agtccggctg gatcgtgccc tgctgggcgg ccacttcccg ggccgtgtgg aggcgcacga
     4381 tgccgtcgaa gcgaggctgg gcgaaccact cgctcgctgc tgcgagctgg gcctcgcggg
     4441 ggctcatcgc gctctcgacg cggtcagggg tcgacaccgg tgtcattcgt tctcgtggtc
     4501 cctgagacaa tcgtcggggc actcgcactc ggcgaggaag ctgtacggac gcccgccggc
     4561 ctgggcgagc gggccgtcat cggccctgac cagccgagga aaccacgggc gcgagcggtc
     4621 agcggaccct gtcctctgcc tggccggttg acgaacatcg acgtcgagtt gcatggaaac
     4681 acaccttcgt tgcgtgggcg gtccaccgta cgctcctcgc gtcgatcagt ccaatcgatt
     4741 gtatggtagg ttttgatcaa ttagactgat caccaatgga ccggtgccat gctcctcgcg
     4801 cagatcgaag ggttcgtcga gatcgcccgc gtggggacca tgcggcgtgc ggcggatgcc
     4861 ctgttgatca gccagccggc actgaccgcg cggctccagg cgctcgagga ggagctcgcc
     4921 gcgcccctct tccggcggac ccacaccggg atggtcctga ctccggctgg tcgcgcgttc
     4981 ctgccgcatg ccgagcgcgc gatggaggcg atccggagcg gcggctcgct cgtccgggag
     5041 ctcgagcacg gcgttgtggg ggagctggcc cttgcggtcg ctccggcggt cagcgcctac
     5101 gtcctgccgg agatcctcgt ccgcttcacg gagcgacatc ccgatgttcg gctgcgcgtg
     5161 cggaccggcc attcggagga gatcgtcgac ctcgtcgttc gcggggaagt cgagctcgga
     5221 atcgtgcgca agctccgcga cgggcgggtc cgcagccggg ccctgtacga ggacgagctc
     5281 gtcctcgtgg ctcgtccgga ccatccattc gccgccgctg gcaacgtgga cgtgtccgag
     5341 atcagtcacg tccagctcat cctgttcgac cgaacctcga gctactacga ggtcacaagc
     5401 gcgctgttcc gcgctgccgg cgtcgttccg cgcggcatca ccgaggtgga caacatcgag
     5461 gcggcgaaac ggatggtcga tcgcgggctc ggc
//
LOCUS       DATAIW010000429         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_251326, whole genome shotgun sequence.
ACCESSION   DATAIW010000429 DATAIW010000000
VERSION     DATAIW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251326"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..830
                     /locus_tag="VHM48_12885"
     CDS             315..830
                     /locus_tag="VHM48_12885"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX2756357.1"
                     /translation="MARGAKDNHHTDRRSSTLGRALGLMAFMPLASRAPLYARLMLAL
                     LVDERTPAARKALLAGAAGYLLVGRDLISDNIPILGGLDDLVVVVLAVDLFLDGVPAD
                     LLDEKLVELDIDRVAFDQDIARIRRLTPGPVRKTIRRIPELLGQASAALRQSGVGPRV
                     RNWINREERTA"
     gene            827..1273
                     /gene="rplI"
                     /locus_tag="VHM48_12890"
     CDS             827..1273
                     /gene="rplI"
                     /locus_tag="VHM48_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX2756358.1"
                     /translation="MKVILTKDVDKLGKSGEMKQVADGYATNFLIPNGVAVPAAGGAY
                     RAWQHDIASREDKRVRERADADIAATRIASTTLTMGVKVGEGGKLYGSITSKDIADAL
                     GRRGIEVDRHKIDLEEPLKSLGTYKVAIKVYGGLSPEVTIVVEPKG"
     gene            1578..>1954
                     /locus_tag="VHM48_12895"
     CDS             1578..>1954
                     /locus_tag="VHM48_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756359.1"
                     /translation="MTQSAGPAWNRIILHVDLDAFFAAVEQRDHPELRGKPVIVGGGG
                     PNDRGVVSTASYEARAFGVHSAMSLREAGRRCPDGIFVPVDGAKYQVASKAVMAVLRR
                     FTPLVEPISIDEAFLDVTGSRVL"
BASE COUNT          317 a          672 c          688 g          277 t
ORIGIN      
        1 tggccggaag gcccggcagc gacgggctag tgctcgctgc gcgagcaagt ggcgaagggt
       61 gcgggcgacg agggccggcg acgatggccg gcgacgatgg ccggcgacga tggccggcga
      121 cgaggcgcag gcggcgagaa ccggcgacga agctgcgggc gacgagaacc gccgacgaga
      181 accgccgagc ccgcgccacg gcactccatc agcgcgtcct ctggcggttg gagccggggc
      241 tgatagactc ccgatccccg gggctcggcc gcgtgcgtgc gcgccgacgc cggtacttcc
      301 gcacaagggg tcgcatggcg cgcggggcga aggacaacca ccacacggat cgacgatcga
      361 gcacgcttgg acgcgcgctc ggcctgatgg cgttcatgcc gctcgcgagc cgggcgccgc
      421 tgtacgcccg actgatgctg gcgctgctcg tcgacgagcg cacgccggcc gcgcgcaagg
      481 cgctcctcgc cggagcggcc ggctatctgc tggtcggccg cgacctgatc tccgacaaca
      541 tcccgatcct cggcgggctc gacgatctcg tcgtcgtcgt ccttgccgtc gacctcttcc
      601 tcgatggcgt ccccgccgat ctcctcgacg agaagctggt cgagctcgac atcgatcggg
      661 tcgccttcga ccaggacatc gcccggatcc ggcggttgac gcccggtccg gttcgcaaga
      721 cgatccggcg gatcccggag ctgctcgggc aagcaagcgc ggccttgcgg cagtcgggcg
      781 tcggcccgcg ggttcgaaat tggatcaaca gggaggaacg aaccgcgtga aggtcatcct
      841 gaccaaggac gtcgacaagc tcggcaagag cggcgagatg aagcaggtgg ccgacggcta
      901 tgccacgaac ttcctcatcc cgaacggcgt cgccgtgccc gccgccggcg gcgcctaccg
      961 ggcctggcag cacgacatcg ccagccgcga ggacaagcgc gtccgcgagc gcgccgacgc
     1021 cgacatcgcg gcgacccgga tcgcctcgac caccctgacc atgggcgtca aggtcggcga
     1081 gggcggcaag ctctacggct cgatcacgtc gaaggacatc gccgacgccc tcggccggcg
     1141 cggcatcgag gtcgaccgcc acaagatcga cctcgaggag ccgctgaagt cgctcggaac
     1201 ctacaaggtc gcgatcaagg tctacggcgg actgtcgccc gaggtcacga tcgtcgtcga
     1261 gcccaagggg taggctccgg ccgcgcggcc ggggccggcg gccgacttct ccatccgcgc
     1321 cgcttcctgc caattgcgcg ccaagcgagc atcagcaggg aaatcaaggc cgattgacct
     1381 tcgatcgaat tgcccggtag ctgcgggcag ctaccgaacg ggtgccgcag cggcagtcgc
     1441 cgcggctggg ccatgctgcc gatcgtctgg tgagcatctg acaggcgcgc caggtccttc
     1501 ggtcataggg ggcgggcgcg cgaccgacaa tcgaacatcc ggtcgaaatg ctcgacacgg
     1561 agtgcgatga tcgtcccgtg acgcagtccg ccggaccggc ctggaaccgg atcatcctgc
     1621 acgtcgatct tgacgcgttc ttcgcagcgg tcgagcagcg cgaccatccc gagctgcgcg
     1681 gcaagccggt gatcgtcggt ggtggtggtc cgaacgaccg cggtgtcgtc agcacggcga
     1741 gctacgaggc gcgcgcgttc ggcgttcact ccgcgatgtc cctgcgcgag gccggccggc
     1801 gctgcccaga tgggatcttc gtgccggttg acggagcgaa gtaccaggtc gccagcaagg
     1861 ccgtcatggc cgtcctccgc cggttcacgc cgctcgtcga gccgatttcc atcgatgagg
     1921 cgttcctcga cgtgacgggc tcgcgcgtcc tgtt
//
LOCUS       DATAIW010000430         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_221700, whole genome shotgun sequence.
ACCESSION   DATAIW010000430 DATAIW010000000
VERSION     DATAIW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221700"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VHM48_12900"
     CDS             <1..478
                     /locus_tag="VHM48_12900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756360.1"
                     /translation="AGLVDWWFGGQHVRHLVVRAWFRHFGSTSWLAEDASGAPVGFLV
                     GYRSHDRSTEAILHLVGVHPNHRRRGIGRALVRSFLADVASAGATTATAVAWPGEPIA
                     TAFFRALDFRPDDGPDSQNLFGTPAFPDYEGDGEDRILFVRWLAPGGPDTISSTSP"
     gene            complement(508..1812)
                     /locus_tag="VHM48_12905"
     CDS             complement(508..1812)
                     /locus_tag="VHM48_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756361.1"
                     /translation="MTANHETTVAVGGQRRVPVPDPVARDYLLLALRLDQHIPGLVDG
                     FYGPAELKAQADVEDLRQPARLVEDAAQLLQRIRAEVVEPARRDWLTAQVVALRTQAE
                     ALAGHGLPYFEYVERCFAWRPVRRDEAVFEEAAATIDALLPGPEPLAHRLAAWDDRFI
                     VAPDRLTDVLAWLAARFRARSERLFGLPDGEDVRIRLVTKQPWTGYNWYDGGRQSRFD
                     LNTDLPNRVAELIHVAAHETYPGHHLEHAWKEAELVDRLHRLEASILLINTPECLISE
                     GLADLGQRFASPPDEEADLLMELYRLAGLAIAADAGASRAAAETTVALAAPRRRLTES
                     RVNAALMRHADGVAHDDVLAWLVRVGRYAPPVAAKRLEFIEHPLWRTYVFVYHEGEAL
                     LGRWLEAAPETEQAARFGRLLREELTPTAIAAELAVAELRPG"
     gene            complement(1809..>2054)
                     /locus_tag="VHM48_12910"
     CDS             complement(1809..>2054)
                     /locus_tag="VHM48_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756362.1"
                     /translation="WGSMDRYHLAWLMVGKPKQIVRGRPLTPDEEAAYVREVAEAKTS
                     ALRMSLDAVDRQGMPFVGEEKGMAPGQGMGSVPGERR"
BASE COUNT          273 a          730 c          754 g          297 t
ORIGIN      
        1 cgccggactc gtcgactggt ggttcggcgg ccagcacgtc cgccacctcg tcgtccgggc
       61 atggttccgc cacttcgggt cgacctcgtg gctggcggag gacgcgtccg gggcgccggt
      121 cggcttcctc gtcggctacc ggagtcatga ccggtcgacc gaggccatcc tccacctcgt
      181 cggcgtccat cccaatcatc gacggcgcgg gatcgggcga gccctggtcc gttcgttcct
      241 ggccgacgtc gcgagcgccg gagcgacgac cgcgaccgcc gtggcgtggc cgggcgagcc
      301 gatcgcgacc gccttcttcc gggcgctgga ctttcggccg gatgacggcc ccgacagcca
      361 gaacctcttc gggacgccgg cgtttcccga ctacgagggg gatggcgagg accggatcct
      421 cttcgtccgg tggctcgcac ctggcggccc ggacacgatt tcgagcacat ctccgtagcg
      481 gcgccggctc tcgctcgggc agccctgtca gcccggtcgc agctccgcga cggccagctc
      541 ggccgcgata gcggtcgggg tcagctcctc tcggagcagc cgtccgaacc gggccgcctg
      601 ctccgtctcg ggagcggcct cgagccagcg gccgagcagc gcctcgccct cgtggtagac
      661 aaagacatag gtccgccaca gcggatgctc gatgaattcg agccgcttgg ccgccacggg
      721 cggagcgtac cgaccgaccc gcacgagcca ggcgaggacg tcgtcgtggg caacgccgtc
      781 ggcatggcgc atcagcgcgg cgttgacccg actctcggtc aggcggcggc gcggcgcggc
      841 cagcgcgacc gtcgtctcgg ccgcggcgcg cgaagcgccg gcgtccgcgg cgatcgcgag
      901 gccggccagc cggtagagct ccatgagcag gtccgcctct tcgtcgggcg gtgaggcgaa
      961 ccgctggccg aggtcggcga ggccttcgct gatcaggcac tcgggggtgt tgatcagcag
     1021 gatgctcgcc tcgagccgat ggagccgatc gacgagctcg gcctccttcc atgcatgctc
     1081 gaggtggtga cccggatagg tctcgtgggc ggccacgtgg atgagctcgg cgacgcggtt
     1141 cggaaggtcc gtgttcaggt cgaaccgcga ctgccgcccg ccgtcgtacc agttgtagcc
     1201 ggtccacggc tgcttcgtga cgagccggat ccggacgtcc tcgccgtcgg gcagcccgaa
     1261 gagccgttcg gaacgggccc ggaaccgcgc cgccagccag gccagaacgt ctgtcagccg
     1321 atccggcgcg acgatgaacc ggtcatccca ggcggcgagg cggtgggcga gcggctccgg
     1381 acccgggagc agggcgtcga tcgtcgcggc cgcctcctcg aagaccgcct cgtcgcggcg
     1441 cacaggccgc caagcgaagc agcgctcgac gtactcgaag tacgggaggc cgtggccggc
     1501 gagcgcctcg gcctgggtcc gcagggccac cacctgggcc gtcagccagt cgcgccgtgc
     1561 cggttcgacg acctcagcgc ggatccgctg gagcagctgg gcggcgtcct cgacgagtcg
     1621 ggcgggttgc cggaggtctt cgacgtccgc ctgggccttg agctcggccg ggccgtagaa
     1681 gccgtcgacg agcccgggga tgtgctggtc gaggcggagg gcgaggagga ggtagtcgcg
     1741 ggcgaccggg tccgggacgg gaacgcggcg ctggccgccg acggcgacgg tcgtctcgtg
     1801 attcgcggtc accgtcgctc cccgggcacc gatcccatcc cctggcccgg cgccatgccc
     1861 ttctcctcgc cgacgaacgg catgccctgg cggtcgaccg cgtcgaggct catccggagg
     1921 gcggacgtct tggcctcggc gacctcccgg acataggccg cctcctcgtc gggcgtgagg
     1981 ggccggccac ggacgatctg cttcggcttg ccgaccatca gccaggcgag gtggtagcga
     2041 tccatcgatc ccca
//
LOCUS       DATAIW010000431         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_192247, whole genome shotgun sequence.
ACCESSION   DATAIW010000431 DATAIW010000000
VERSION     DATAIW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192247"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /locus_tag="VHM48_12915"
     CDS             complement(<1..2113)
                     /locus_tag="VHM48_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2756363.1"
                     /translation="MALTSPSAARASGVLSALIERDEPLAALAGYLDEAAAGSGRFVV
                     IRGEAGIGKTALLGEFLRSCPADVTILSGACDGVSTPQPFGPLEDMVAALGPELRALL
                     DADARRAEVGRWLLDRLSGDAVHVLAIDDVQSADEATLELLAWLARRLESLRLLVVVT
                     LREGEGSESSVARMVGAVASLPSVRHLSLIPLTRAGVARLAAGRPVDLDELHRITAGN
                     PFYASEVLTGETDDPDAIPVSILDAVRARVARLDARGQRALQAAAIIGVRAEPWLLAA
                     IAGEDLVGIDDSLRVGLLTKSDGLAFRHELTRRAVLEDLPVIHGIALHRLALAALERA
                     GIGDSARLAYHAEGAADRAAVLRHAAAAGRRALAVGALNEAAEQFRRALESADGRPAD
                     ERAELVESLARVLFLLNHLAEAVELGREAVAIRRQTGDTVMASIDLSVLALAAWTNGR
                     GDEAWQAAREAVALVASSGDSHALALAHATLGRLGLSAGRLDEGRAMSERAVEIGRRI
                     DDPESTAIGLATIGTIDILEGDLAGFASLEESLQIGRRADLPPIVDRALNNMGVSSFT
                     LGRMHDAESWYEEMERHSERSEIERCGIDTPRAEIALALGNWDAAATHARAAFVAPRT
                     DPVDRALGMLVLARLGIRRGEPGWEAWTTEPSEFERRLETSQLRWPIAAVLAEHAWLE
                     GDVDPILPGLREAYAEACAAGD"
     gene            complement(2138..2779)
                     /locus_tag="VHM48_12920"
     CDS             complement(2138..2779)
                     /locus_tag="VHM48_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756364.1"
                     /translation="MTRRRSRLAAAALAAMVLGAGLVGSATAGHSDLNAVRAATARFH
                     SLAAAGKGGFGQPPAPAPLHECISSLDGTGAMGFHYINGSRLDKVLDPTLPEVLVYAP
                     DADGKLHLAALEYVIFQADWYADHAAGTMPTLLGQDMMPNNGARFQIPPFFALHVWLF
                     QDNPSGMFAPFNPDVSCGSASAAVRGSGSTAAAAVATADVARTWACEVPARSA"
     gene            complement(2782..3033)
                     /locus_tag="VHM48_12925"
     CDS             complement(2782..3033)
                     /locus_tag="VHM48_12925"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX2756365.1"
                     /translation="MALFMDTHTIDGGVKASDVAQAHLADLQTQDRYGVRYLRYWVDE
                     GAGKIYCLVDAPDADAANRVHREAHGLVAQSISPVIEGE"
     gene            complement(3259..5112)
                     /locus_tag="VHM48_12930"
     CDS             complement(3259..5112)
                     /locus_tag="VHM48_12930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX2756366.1"
                     /translation="MRARTPLTAALVAVSLLTLALPSAFAASPKYDITTLAGKAASLH
                     GTAGLQVRFSAFLEEEATGAENQTPPPGPTTPITPSFVLSSPLDGTSIAGPNVLVNQD
                     TAGAPQNETSIAVDPNNPNRVVASANDYVTRTWTCTINGTPCSALGDGYSGTYYSNDG
                     GQTWCCASSDPAHLGTLIPGVTRLAGGVYDAAGDPAVAFDSRGHVFYAGLGFNRASAP
                     NTVAVNKGTFDGSGHLSWAAPVFINPTTAPSTLNDKEWIAVDSHASSQFRDNVYVTWT
                     RFKFNGHTGAYTQSPIFFVRSTDGGVTYSAPKAISANILYSQGSRPVVGPDGTLYVFW
                     DGSTRLASLNSTWMVKSTDGGATFSKPVAISRLTDTDVLKNTAFRVNSFPAAAAAPNG
                     DLYATWTTEALTSGTPNLAGETACADWLGGNPEACYSVAVYSKSTNGGSTWSSPAPVF
                     APANRTAIGYPVTQPNGDTFGAPAALPAEQVFPAVAVAPNGRVYLSAYSADVVSPWQT
                     CDAGPPPPPGRIACDTLGSYIHNARLNYKVADLTTGVTRNVNTHPINTRHGFGGGFFG
                     DYTDIAVGSDNVFHALWTDSNNRQTVVWFYGFEFTPTSINQEEIVTATGSF"
BASE COUNT          776 a         1759 c         2004 g          791 t
ORIGIN      
        1 gatcgcccgc cgcgcaggcc tcggcgtagg cctcccggag cccggggagg atcggatcga
       61 catcgccctc gagccaggca tgctctgcca gcaccgcggc gatcggccat cgcagttggg
      121 acgtctccag gcggcgctcg aactcgctcg gttcggtggt ccaggcttcc cagcccggct
      181 cgccgcggcg gatccccagc ctggcgagga cgagcatgcc gagggcccgg tcgaccgggt
      241 cggtccgggg cgccacgaac gctgcgcggg cgtgggtcgc ggccgcgtcc cagttgccga
      301 gagcgagggc gatctccgcg cgaggcgtat cgatgccgca gcgctcgatc tcggagcgct
      361 cgctgtggcg ctccatctcc tcgtaccagg actcggcgtc gtgcatccga cccaacgtga
      421 acgacgagac acccatgttg ttgagggccc gatcgacgat cggcgggagg tccgcccggc
      481 gcccgatctg gagcgactcc tcgagggacg cgaagccggc gaggtcgcct tccagaatgt
      541 cgatcgtgcc gatcgttgcg aggccgatcg ccgtcgactc cgggtcgtcg atccgccggc
      601 cgatctcgac ggcccgttcg ctcattgccc gcccttcgtc gaggcgtccg gcactcaacc
      661 ccaatcgtcc gagggtcgca tgggccagcg ccagggcgtg gctgtcgccg gacgatgcga
      721 ccagggccac cgcctctctg gccgcctgcc aggcctcgtc cccgcggccg ttcgtccatg
      781 ccgcgagggc caggaccgac aggtcgatcg acgccatcac ggtgtcgccg gtctggcgcc
      841 gaattgccac ggcctcgcgg ccgagctcga cggcctctgc caggtggttg agcaggaaca
      901 ggacccgcgc cagcgactcg acgagctccg cgcgctcgtc ggccggccga ccatcggcgc
      961 tctccagggc gcgccggaac tgctcggccg cctcgttgag ggcgccgacg gcgagggccc
     1021 gccgccccgc cgctgccgca tggcggagca ccgccgcgcg atcggcggct ccctcggcgt
     1081 gataggcgag ccgcgcgctg tcgccgatcc cggcccgttc gagggctgcg agggccaggc
     1141 ggtgaagggc gatgccgtgg atgaccggga gatcctccag cacggctcgc cgggtgagct
     1201 cgtgacggaa ggcgaggccg tcggacttcg tcagcaggcc gacccgaagg ctgtcatcga
     1261 ttccgacgag gtcttcgccg gcgatcgcgg ccagcagcca cggctcggcc cggacgccga
     1321 tgatcgcggc cgcctggagg gcacgctggc cgcgtgcgtc gagccgggcg acccgggccc
     1381 ggacggcatc gaggatcgac acggggatcg cgtccgggtc gtctgtctcg ccggtcagca
     1441 cctccgaggc atagaagggg ttgcccgccg tgatccggtg gagctcgtcg agatcgaccg
     1501 ggcgcccggc cgcgagccgt gcgacgccgg cgcgcgtgag cgggatcagc gacaggtggc
     1561 ggacactggg caacgacgcg acggcgccga ccatccgggc gacgctggat tccgaccctt
     1621 cgccctcgcg cagggtcacg acgaccagca gccgcagcga ttcgagtcgc cgggcgagcc
     1681 aggcgaggag ctccagcgtg gcctcgtcgg ccgattggac gtcgtcgatg gccaggacgt
     1741 ggacggcgtc cccggacagc cggtcgagca gccaccgccc gacctccgcc cgccgcgcgt
     1801 cggcgtcgag cagcgcgcgc agctccggcc cgagcgcggc gaccatgtcc tcgagcggcc
     1861 cgaacggttg cggggtcgat acgccgtcgc aggcaccgga caggattgtc acgtccgccg
     1921 gacacgagcg aaggaattcg cccaggagcg cggtcttgcc gatcccggcc tcgccccgga
     1981 tcacgacgaa ccggccgctc ccggcggccg cctcgtcgag gtagccggcc agggcggcca
     2041 gcggttcgtc gcgttcgatc agcgccgaca ggacgccgga tgcacgggct gcgctgggag
     2101 aagtgagggc catgcgcgaa cgatagcggg gatcggctca ggcggaccgg gccggcacct
     2161 cgcacgccca tgtgcgggcg acgtcggccg tcgctaccgc agccgcggcc gtcgaaccgg
     2221 agccgcggac cgccgcggac gccgagccgc atgacacgtc cggattgaac ggcgcgaaca
     2281 tgcccgacgg attgtcctgg aacagccaga cgtggagggc gaagaacggt gggatctgga
     2341 agcgagcccc gttgttcggc atcatgtcct ggccgaggag cgtcggcatc gtcccggcgg
     2401 cgtggtcggc gtaccagtca gcctggaaga tgacgtactc gagcgccgcg agatggagct
     2461 tgccgtcggc gtcgggggcg tagacgagga cctccggcag ggtcgggtcg aggaccttgt
     2521 cgagcctcga tccgttgatg tagtggaagc ccatcgcgcc ggtgccgtcg agcgacgaga
     2581 tgcactcgtg gagcggcgcg ggggccggcg gctggccgaa accacccttg ccggcggcgg
     2641 ccaggctgtg gaaccgcgcg gtggcggcgc ggaccgcatt gagatcggag tggccggcgg
     2701 tcgcgctacc gaccaggccc gcgccgagga ccatcgccgc gagcgcggcc gcggcgaggc
     2761 gggagcgtcg tcgagtcatc gtcattctcc ttcgatcacc gggctgatcg actgggcgac
     2821 caggccgtgg gcctcgcggt ggacgcggtt ggccgcgtca gcatccgggg cgtcgacaag
     2881 gcagtagatc ttgcccgccc cctcgtcgac ccagtaccgg agatagcgga cgccgtagcg
     2941 gtcctgggtc tggagatcgg cgaggtgggc ctgggcgacg tcgctggcct tgacccctcc
     3001 gtcgattgtg tgggtgtcca tgaagagcgc catgcggtgc ctcctttcgc ttcggaggat
     3061 cgcggcgcct gcccgccggg cgcatcggtc ggatgaccga tgttggtcgc cgcggttacc
     3121 cacgttgggc gccgggccgg ccgaggggcc cgatgatcac gcacggacgc ggaggaatga
     3181 cgcggaggaa tgacgcggag gaatcgcgaa gcccgcgggc cggctgctgc cgactcgcgg
     3241 gctcgggaga cgcgatcgct agaaggatcc ggtcgcggtg acgatctcct cctggttgat
     3301 cgaggtcggc gtgaactcga acccgtagaa ccagaccacg gtctggcggt tgttgctgtc
     3361 cgtccagagg gcgtggaaga cgttgtcgga accgacggcg atgtcggtgt aatcaccgaa
     3421 gaagccgccg ccgaagccgt ggcgggtatt gatgggatgg gtgttgacgt tccgcgtgac
     3481 gcccgtcgtc aggtcggcga ccttgtagtt caggcgtgcg ttgtggatgt agctcccgag
     3541 cgtgtcgcag gcgatccggc cggggggcgg cggcggtccg gcgtcgcagg tctgccacgg
     3601 cgagacgaca tcagccgaat aagccgagag atagacccgt ccgttcgggg cgaccgccac
     3661 cgccgggaag acctgctctg ccggaagcgc ggccggggcg ccgaacgtgt cgccgttcgg
     3721 ctgcgtcacc ggatagccga tggcggtccg gttcgccggg gcgaacaccg gtgcaggaga
     3781 cgaccacgtc gagccgccgt tggtcgactt gctgtagacg gccacggagt agcacgcctc
     3841 ggggttaccg ccgagccagt cggcgcaggc ggtctcaccg gcgaggttcg gcgtgccgct
     3901 ggtcaaggcc tcggtcgtcc aggtcgcata aaggtcaccg ttcggcgccg cggcggcagc
     3961 cgggaagctg ttgacgcgga acgcggtgtt cttgaggacg tcggtgtcgg tcagccgcga
     4021 gatcgcgacc ggcttgctga acgtcgctcc gccgtcggtc gacttgacca tccaggtgct
     4081 gttgagcgac gcgaggcggg tcgacccgtc ccagaagaca tagagcgtcc cgtccggccc
     4141 gacgaccggt cgcgagccct ggctgtacag gatgttggcg gagatcgcct tcggtgcgct
     4201 gtaggtcact ccgccgtcgg tcgagcgaac gaagaagatc ggcgactgcg tatacgcgcc
     4261 ggtgtggccg ttgaacttga accgggtcca ggtcacgtag acgttgtcac ggaactggct
     4321 cgaggcatgg ctgtcgaccg cgatccattc cttgtcgttc aacgtcgagg gcgccgtcgt
     4381 cgggttgatg aagacgggcg cggcccagga gaggtggccg ctgccgtcga atgtgccctt
     4441 gttgaccgcg acggtgttcg gggccgacgc ccggttgaac ccgaggccgg cgtagaagac
     4501 atggccgcgg ctgtcgaagg cgacggccgg atcgcccgcg gcgtcgtaga caccaccggc
     4561 gaggcgagtg acgccgggga tcagcgtgcc gagatgggcc gggtcgctgc tcgcgcagca
     4621 ccaggtctgg ccaccgtcat tggagtagta cgtccccgaa tagccgtcgc cgagggcgct
     4681 gcacggcgtt ccgttgatgg tgcaggtcca cgtccgggtg acgtagtcgt tcgcggatgc
     4741 gacaacccgg ttcgggttgt tcggatcgac cgcgatcgac gtctcgttct gcggtgcgcc
     4801 ggcggtgtcc tggttcacga gaacgtttgg cccggcgatg gacgtcccgt cgagcgggct
     4861 cgacaggacg aagctcgggg tgatcggggt cgtcgggccg gggggcggag tctggttttc
     4921 tgctccggtg gcctcctcct cgaggaacgc cgagaaccgg acctgcaggc cggcagtccc
     4981 gtgcagggac gcggccttgc cggcgagcgt cgtgatgtcg tacttcggcg aggcggcgaa
     5041 cgcggatggc agggccaagg tcagcaggga cactgcgacc agggctgcgg tgagcggggt
     5101 gcgtgctctc acgatggtgc tcctcctgtg gggcatgggt gcgcgtccac gatggagggt
     5161 cggacgccgt ctcccgccgg cgacccgagc tggcggtcag gcagccatgc gtggtccgcg
     5221 atgagtccgg gctaccgtac tacggggccg acgaagcggc attgcacgcg aaaaccggga
     5281 ccgaccgatc cgacgtttcc ccgagcagcg agcgtttgcc ggtctcttgc
//
LOCUS       DATAIW010000432         6413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_192648, whole genome shotgun sequence.
ACCESSION   DATAIW010000432 DATAIW010000000
VERSION     DATAIW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192648"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VHM48_12935"
     CDS             <1..1097
                     /locus_tag="VHM48_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2756367.1"
                     /translation="AESIDATVRKYGGTIAVGDVSFDVEPGEVFVVMGLSGSGKSTLV
                     RCLTRLIEPTFGEVDLDGEDIRKASQERLRELRRRRFSMVFQNFGLLPHRRVLDNIAF
                     GLELRGEPKAQRLARAEEMLGLVGLDGMADRYPEQLSGGQQQRVGLARALAGDPEVML
                     LDEPFSALDPLIRRDMQNEVIRLHDDLRKTMVFITHDLAEALKLGDHVMIMRAGKVVQ
                     IGRPEELVARPADAYVEDFVRDIPKAHVLTLRWVMRDATADDPLDGPVFSPDTIIRTV
                     IHAAASIDKPIRVVDEGRLVGIVDRANILEAIGGADPGGGPSVAGTDPGGPPYHSPRE
                     RVEPIRKPTEATTRFLADAVAEEAEPPEPR"
     gene            1094..3022
                     /locus_tag="VHM48_12940"
     CDS             1094..3022
                     /locus_tag="VHM48_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2756368.1"
                     /translation="MTAISLSARSRLDPLTARRGLMLVLLAAAAIAYVLFRNQWTLPH
                     NDDEPLFRSLNSVRDFVDENRNVLEPIRLAIGSVVDAFDFVIESLGWPGIIGLAGALG
                     LVFGGWRLAVLTITGFASLGVLGLWDASMATLGLMLAAVILAVAIGVPLGILAGRSQR
                     ASAILAPILDVMQIMPTFAYLTPMTLLFFIGAAPSVVATLIYAIPPAIRITALGIRGV
                     PSGSVEAAMSLGSTGRQVLTKVQLPLARRAIGLGINQSIMMALSMVVITAMIGAPGLG
                     RNILNAVQRVDVGAAFDAGLAIVILAIILDRVTYAAGDWLDPRARIRTLRSGRQRLVG
                     WLGPLGLIGAGLLAPFAIDATQFPTAVQFSFRGSVNESVNWFTDTFSRATIVIKDVST
                     NLVFNPLEGLLTSSPWWLVVAVTMLIAWYLSGRRASIAAGICLALIVLLGLWGHSMAT
                     LANVLVGTALTLVIGIAFGILTARSDRLRAILRPILDAAQTLPAFVYLIPALALFQST
                     RFTAVVAAVIYAVPPVIRLVDVGIRSVPETVVEAATMSGATERQLLWKVRLPLSMRAL
                     LLAANQGIVLVLAMVVIGGLVGAGALGYDVIAGLAQGKDFGKGLAAGIAIVLLGIMLD
                     RITQGAGSRRRTTVAKAG"
     gene            3053..4090
                     /locus_tag="VHM48_12945"
     CDS             3053..4090
                     /locus_tag="VHM48_12945"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2756369.1"
                     /translation="MQRNRWGRWFAGLAVVSLIVVGCAKSSGATTPPASTAAGASTGT
                     TASSGPSASATVAAATCQGSGDKGSVKMMVNEWVGAAANVAVAQCLLQQMGYKVETST
                     LAEEIAWQGFDTGEVDVILENWGHPALEKKYIKDTKLATDAGSNGVTGIIGWYVPKWM
                     VDEHPDITDWNNLNKYASLFKTSESGDKGQFLGANPTFVQYDEALITNLHLNYKVVFS
                     GSEAATITAFQQATKNKTPLIGYFYDPQWLLSEIELVQVKLPAWTQGCDADLKKVACD
                     YPAYTLNKIVRTKWMDAAGDAATFIKNFKWKNSDQNLVADAITNKSMKELDAAKQWID
                     ANAATWQAWMP"
     gene            complement(4402..5391)
                     /locus_tag="VHM48_12950"
     CDS             complement(4402..5391)
                     /locus_tag="VHM48_12950"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX2756370.1"
                     /translation="MATDNSPHAAAPTSPPAHTRESTADDRALLNELEPEAARLYDRH
                     ARVAQEWFPHDYIPYRLGRDFDKEPWTPDQPRLTGVAQTAFEVNLLTEDNLPSYHRLI
                     HGMFGAGDGAWINWIGRWTAEEGRHAIVLRDYLTVTRNLDPVALERGRMTQLQQGYDR
                     DTIDTLRGLAYVAFQELATRISHKNTGRYSQDPVADRIMTRIAADENLHMVFYRDILG
                     AALKIQPSRAVQAIVAEVLAFEMPGNGIPGFLRKAAAIAKAGIYDIRIHRDEVLLPII
                     NHWKIFELTGLDAAAEEARKRLAQHLDKLEVAAKRFEEKMALATGNARLIRAG"
     gene            5820..6152
                     /locus_tag="VHM48_12955"
     CDS             5820..6152
                     /locus_tag="VHM48_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756371.1"
                     /translation="MKIAITVGFALVASLIGACSTGLGPTSTTATPDCRSFGGETVDA
                     VGWSSAGDFLAVSTSSDSDGQGRIRVFNWPAMSLASDAKTDVLAVDDAAVDDEGAVLL
                     VFVGPDGR"
BASE COUNT          976 a         2176 c         2202 g         1059 t
ORIGIN      
        1 gcgccgaatc gatcgacgcc acggtccgca agtacggcgg gaccatcgcc gtcggcgacg
       61 tctcgttcga cgtcgagccc ggcgaggtct tcgtcgtcat gggcctgtcc ggcagcggca
      121 agtcgaccct cgtccgctgc ctcacccgtc tgatcgagcc gacgttcggc gaggtcgatc
      181 tcgacggcga ggacatccgc aaggcgagcc aggagcgcct ccgggaactg cggagacgcc
      241 gcttctcgat ggtcttccag aacttcggcc tgcttcccca tcggcgggtc ctcgacaaca
      301 tcgcgttcgg gctcgagctg cgcggcgagc cgaaggccca gcgcctcgcc cgggccgagg
      361 agatgctcgg cctcgtcggg ctcgacggga tggccgaccg ctacccggag cagctgtcgg
      421 gcggccagca gcagcgggtc gggctggccc gggccctcgc cggcgatccg gaggtcatgc
      481 tcctcgacga gccgttcagc gccctcgatc cgctgatccg gcgcgacatg cagaacgagg
      541 tgatccgtct ccacgacgac ctgcgcaaga cgatggtctt cattacccac gacctcgccg
      601 aggccctcaa gctcggcgac cacgtgatga tcatgcgggc cggcaaggtg gtccagatcg
      661 ggcggcccga ggagctcgtc gcgcggccgg ccgacgcgta cgtcgaggac ttcgtccgcg
      721 acatcccgaa ggcgcacgtc ctcaccctgc gctgggtgat gcgcgacgcg accgcggacg
      781 acccgctcga cggcccggtc ttctcgcccg acacgatcat ccgcacggtc atccacgccg
      841 cggcctcgat cgacaagccg atccgggtcg tcgacgaagg gcggctggtc ggcatcgtcg
      901 accgggcgaa catcctggag gcgatcggcg gcgccgatcc gggcggcggg ccgtccgtcg
      961 ccggcacgga cccgggcggt ccgccgtatc actcgccgcg cgagcgggtc gaaccgatcc
     1021 gcaagccgac cgaggcgacg acccggttcc tggccgacgc ggtcgccgaa gaggccgagc
     1081 cgcccgagcc gcgatgaccg cgatcagcct atcggcgcgg agccggcttg acccgctgac
     1141 ggcccgacgg gggctgatgc tcgtcctgct cgcggcggcg gcgatcgcct acgtcctgtt
     1201 ccgcaaccag tggacgctcc cgcacaacga cgacgagccg ctcttccggt cgctcaacag
     1261 cgtccgcgac tttgtcgatg agaatcgcaa cgtgctcgag ccgatccggc tcgccatcgg
     1321 ctccgtcgtc gacgccttcg acttcgtcat cgagtcgctc ggctggccgg ggatcatcgg
     1381 cctggcgggc gccctggggt tggtcttcgg cggctggcgg ctggcggtgc tgacgatcac
     1441 cggcttcgcc tcgctcgggg tcctcggtct gtgggacgcg agcatggcga cgctcggcct
     1501 gatgctcgcc gcggtcatcc tcgcggtcgc catcggggtc ccgctcggaa tcctggccgg
     1561 ccgcagccag cgagcctcgg cgatcctcgc gccgatcctc gatgtgatgc agatcatgcc
     1621 gacgttcgcc tacctcacgc cgatgaccct gctcttcttc atcggcgcgg cgccatcggt
     1681 ggtcgcgacc ctgatctacg ccatcccgcc ggcgatccgg atcaccgccc tcgggatccg
     1741 gggcgtgccg tcgggctcgg tcgaggcggc gatgtcgctc ggctcgacgg gccgtcaggt
     1801 cctgaccaag gtccagctgc cgctcgctcg ccgggcgatc gggctcggga tcaaccagtc
     1861 gatcatgatg gccctctcca tggtcgtgat cactgcgatg atcggagcgc ccgggttggg
     1921 gcggaacatc ctcaatgccg tccagcgggt cgatgtcggt gcggccttcg acgccgggct
     1981 cgcgatcgtg atcctcgcga tcatcctgga ccgggtgacc tacgccgccg gcgactggct
     2041 ggacccgcgc gcccggatcc ggacgctgcg gtccggccgc cagcggctcg tcggctggct
     2101 cggcccactc ggcctgatcg gcgccggtct gctcgcgccg ttcgcgatcg acgccacgca
     2161 gttcccgacc gccgtgcagt tctcgttccg gggctcggtc aatgagtccg tcaactggtt
     2221 caccgacacg ttttcgcggg ccacgatcgt catcaaggac gtgtcgacga acctcgtctt
     2281 caacccgctc gagggcctcc tcacgtcgtc gccgtggtgg ctcgtcgtgg ccgtcacgat
     2341 gctgatcgcc tggtacctgt ccggccgacg agcgtcgatc gccgccggca tctgcctcgc
     2401 cctgatcgtc ctgctgggcc tgtgggggca ctcgatggcg accctcgcca acgtgctcgt
     2461 cggcactgcc ctgacgctgg tcatcgggat cgcgttcgga atcctcaccg cacggagcga
     2521 tcggctccgg gcgatcctcc gaccgattct cgatgccgcc cagacgctgc cggccttcgt
     2581 ctacttgatc ccggcgctcg cgctcttcca atcgacccgt ttcaccgccg tggtcgccgc
     2641 cgtgatctac gccgtgccgc cggtcatccg cctggtcgat gtcgggattc gttcggtgcc
     2701 cgaaacggtc gtcgaggccg ccacgatgtc aggtgccacc gaacgccagc tgctgtggaa
     2761 ggtgcgcctg ccgctgtcga tgcgggcgct cctgctggcg gcgaaccagg ggatcgtcct
     2821 ggttctcgcg atggtcgtga tcggcggact cgtcggcgcg ggcgcgctag gctacgacgt
     2881 catcgccggc ctcgcccagg gcaaggactt cggcaagggc ctcgcggccg gcatcgcgat
     2941 cgtcctgctc gggatcatgc tggatcggat cacccagggc gcaggaagcc gtcgcaggac
     3001 caccgtcgcg aaggccggct agggccggca accagaggag gagcgaatcg tcatgcagcg
     3061 aaatcgatgg ggtcgctggt tcgccgggtt ggccgttgtc agcctgatcg tggtcggctg
     3121 tgccaagtcg tcgggcgcga cgacaccgcc ggcaagcacc gcggccgggg cgagcaccgg
     3181 cacgacggcc agcagcggac cgagcgcgag tgccaccgtg gccgccgcga cctgccaggg
     3241 cagcggcgac aagggcagcg tcaagatgat ggtcaacgag tgggtcggag cggcggccaa
     3301 cgtcgcggtc gcccagtgcc tgctccagca gatgggctac aaggtcgaaa cgagcaccct
     3361 cgccgaggag atcgcctggc agggcttcga caccggcgag gtggacgtca tcctcgagaa
     3421 ctggggccac cccgcgctcg agaagaagta catcaaggac acgaagctgg ccacggacgc
     3481 cggatcgaat ggcgtgaccg ggatcatcgg ctggtacgtc ccgaagtgga tggtcgacga
     3541 gcatcccgac atcaccgact ggaacaatct caacaagtac gcctccctgt tcaagacgtc
     3601 cgagtcgggc gacaagggcc agttcctcgg cgccaacccg acgttcgtcc agtacgacga
     3661 ggcgctgatc accaaccttc acctgaacta caaggtcgtc ttctcgggga gcgaggcggc
     3721 gacgatcacg gccttccagc aggccaccaa gaacaagacg ccgctcatcg gctacttcta
     3781 cgacccgcag tggctcctca gcgagatcga gctcgtccag gtcaagctgc cggcctggac
     3841 ccagggctgc gacgccgatc tgaagaaggt cgcgtgcgac tatccggcct acaccctgaa
     3901 caagatcgtc cggacgaagt ggatggacgc ggccggcgat gcggccacgt tcatcaagaa
     3961 cttcaagtgg aagaacagcg accagaactt ggtcgccgat gcgatcacga acaagagcat
     4021 gaaggagttg gacgccgcca agcagtggat cgacgcgaat gccgcgacct ggcaggcgtg
     4081 gatgccctga tcgactgagc gcgcgggtcg tcgccgtgcg gcggcccacc cagacctcga
     4141 cggcggcggg cggtccattc cgtccgccgt cgcgattcaa cccgtcgatg aaccggctcg
     4201 acggccccgc cgcaacgaac tcgtgcggct cgcgccgatc acggtccaca tcgagaagta
     4261 gcccggtccc gtccgcggcg tcgcggggaa cgtggaggag gcagggaagg cgtgtcccgg
     4321 cccggggacg atcgcggctc tcgaggactc gtccccgtgc ttgagctcct cgacgcggac
     4381 tggggccgcc ggcgcgtggc ctcagccggc ccggatcagc cgcgcgttgc cggtggcgag
     4441 ggccatcttc tcctcgaacc gcttggccgc gacctcgagc ttgtcgaggt gctgcgcgag
     4501 ccgcttgcga gcctcctcgg cagccgcgtc gagcccggtc agctcgaaga tcttccagtg
     4561 gttgatgatc ggcagcagca cttcgtcgcg gtggatccgg atgtcgtaga tgccggcctt
     4621 ggcgatcgcc gcggccttgc gcaggaagcc ggggatgccg ttgccgggca tctcgaaggc
     4681 gaggacctcg gcgacgatcg cctggaccgc ccgcgacggc tggatcttca gggccgcgcc
     4741 aaggatgtcg cggtagaaca ccatgtgcaa gttctcgtcg gccgcgatcc ttgtcatgat
     4801 cctgtcggcc acaggatcct gcgagtagcg cccggtgttc ttgtgcgaga tccgggtcgc
     4861 gagctcctgg aacgcgacgt aggcgaggcc gcgcagcgtg tcgatcgtgt cgcggtcgta
     4921 gccctgctgg agctgcgtca tccggccccg ctcgagcgcg accgggtcga ggttccgggt
     4981 caccgtgagg tagtcgcgca ggacgatcgc gtggcggccc tcctcggccg tccagcgccc
     5041 gatccagttg atccaggcgc cgtcgccggc cccgaacatc ccgtggatga gtcggtggta
     5101 cgagggcagg ttgtcctcgg tcaggaggtt gacctcgaac gcggtctggg cgacgccggt
     5161 cagacgcggc tggtcgggcg tccagggctc tttgtcgaag tcgcgcccga gacggtatgg
     5221 gatgtagtcg tgggggaacc actcctgggc gaccctggca tggcggtcgt acaggcgggc
     5281 ggcctcgggc tcgagctcgt tgaggagggc gcggtcgtct gcggtcgact cccgggtgtg
     5341 ggcgggcggg gatgtcggcg cggcagcgtg cggggagttg tcggtcgcca tcggtcggtc
     5401 cttgctcaga gaagggccgc gcggcgccca tagcggacgc cggttcaccg accggtcagg
     5461 agtcaagggt agcccggatt gtcatgtcgc cccttcatct cggtgcggac ctgcacatca
     5521 acacgacgtt gcagcgttcg tcgtggccgc gggggtcttc tagcgagggc gcgtttgcat
     5581 cagcaacaga ccacgaggac gtgccaccta acgtccggca caagtcccct tcctcccacg
     5641 agtcgtcgtc gtgcccgacg gatacccgct gccggggact ggctggaaga aggtcagcct
     5701 cgagaagagc gcgctcgtgg cacgccgaca cccgtttcga gggtcgccct cctttccttg
     5761 acttctgacc tgggcccaat atcatgctac tagaagatag caagtatatt gagggccaga
     5821 tgaagatcgc gatcacagtg gggttcgccc tcgtcgcgag tctgatcggc gcgtgttcga
     5881 ccgggctagg cccaacgagc acgacggcca cgccagactg tcgctccttc ggcggcgaga
     5941 ccgtggatgc cgtcggctgg tcatcagctg gcgacttcct cgcggtcagc acatcgtctg
     6001 acagcgacgg ccaaggccgg atccgtgttt tcaactggcc cgcaatgagt ttggccagcg
     6061 acgccaagac cgatgtcctt gctgtcgatg acgcagcggt cgacgacgaa ggggccgttc
     6121 tactggtttt cgtgggaccc gatggcagat gacgcttcgg caaggcaact ctggaaggtg
     6181 gcacccggcg acccgccgga gacaattggc gggccggttc ctgcgggccc ctacgtcggc
     6241 ctcgtctggg ctggcggcag cctcatcgcg atggaggctg acccaggccc gccagagcgg
     6301 tccaggttgg tcatgttcga gactgcccat ccgggcgcga agcccgttgg cgtgaccgat
     6361 tggacgccga ggttgtggtc cactttctgg gccgaccgca ggggcgagtg gat
//
LOCUS       DATAIW010000433         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_251718, whole genome shotgun sequence.
ACCESSION   DATAIW010000433 DATAIW010000000
VERSION     DATAIW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251718"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VHM48_12960"
     CDS             complement(<1..1199)
                     /locus_tag="VHM48_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2756372.1"
                     /translation="MAAPEIDQRGGPDQLEQLRFLHQVARLATTARTWDELLETVVDG
                     TRDALHADVTSLYLLDRDGAYLTLAATNGLDRYQIGRARVPFGEGVTGRVAASREPLV
                     IPDVRSDPRFLWVRGIDQRRFIASMLSVPLSWNDQVVGVLNVQTERERQFSDADVAQL
                     QAIADLLAGIVEKGRQQTEAEERLEQLRQIDEARSELIALVTHELRTPLAVVRAYTEL
                     LGDDPPLEGRESRDPDRRAQRQAWHDAAMDQVERLDRLVDSILASVRIVPAEAAEVTP
                     TDVEAAIAEVVASIRPIVRQHRLDVRPGLGHHALADAPRLRQILEHLIENAVKYAPPE
                     TTITIDHALVEGFVRIGVTDQGPGVPAEWRERIFEPYARRETHTARGSGIGLYAAKRL
                     GESMGARL"
     gene            1314..>1710
                     /locus_tag="VHM48_12965"
     CDS             1314..>1710
                     /locus_tag="VHM48_12965"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX2756373.1"
                     /translation="MPRSADANTGRPDARPPGAGTHAASATKPVAGAGTHAAGDANEA
                     VRAEPATIGTGRARDPLALEIRLLGSLLGQVIAEQAGRDLLDLVERVRRTTIRLRRGD
                     DPALRAGLVAELAALDPDRAEVVVRAFSLY"
BASE COUNT          231 a          604 c          648 g          227 t
ORIGIN      
        1 aggcgggccc ccatcgattc gccgaggcgc ttggcggcgt acagcccgat cccggagcct
       61 cgggcggtgt gggtctcgcg ccgggcgtac ggctcgaaga tccgctcgcg ccattccgcg
      121 gggacgccgg gcccctggtc ggtgacgccg atccggacga agccctcgac gagggcatgg
      181 tcgatcgtga tcgtcgtctc cggtggcgcg tacttgaccg cgttctcgat caggtgctcg
      241 aggatctggc ggagccgcgg cgcgtcggcg agggcgtggt ggccgagccc gggtcggacg
      301 tcgaggcgat gctggcggac gatcgggcgg atcgacgcga cgacctcggc gatggccgcc
      361 tcgacatcgg tcggggtgac ctcggcggct tcggccggca cgatccggac cgaggcgagg
      421 atcgagtcga cgagccggtc gagccgctcg acctggtcca tcgccgcgtc gtgccaggcc
      481 tggcgctggg cgcgtcgatc cggatcgcgc gactcgcgac cctcgagcgg cggatcgtcg
      541 ccgaggagct ccgtgtaggc gcgaacgacc gccagcggcg tccggagctc gtgggtgacg
      601 agggcgatca gctcggaacg cgcctcgtcg atctggcgga gctgctcgag gcgctcttcc
      661 gcctcggtct gctggcggcc cttctcgacg atcccggcga ggaggtcggc gatcgcctgg
      721 agctgggcga cgtccgcgtc gctgaactgc cgctcgcgct cggtctggac gttgaggacg
      781 ccgacgacct ggtcgttcca gctcagcggg acgctcagca tcgaggcgat gaaccggcgc
      841 tggtcgatgc cgcggaccca caggaaacgc gggtcggaac ggacgtcggg gatgacgagc
      901 ggctcacgac tggcggcgac ccggccggtg acgccctccc cgaacgggac ccgcgcccgg
      961 ccgatctggt agcgatcgag gccgttcgtc gcggcgaggg tcaggtaggc tccatcgcga
     1021 tcgagcaggt agagcgaggt gacatcggcg tggagcgcgt cccgggtccc gtcgacgacg
     1081 gtctcgagca gctcgtccca ggtccgggcg gtcgtcgcga gacgggcgac ctgatgcagg
     1141 aaccgaagct gctcgagctg atccggtccg ccccgctggt cgatctcggg cgccgccatg
     1201 gccgtcacga tacaagccta cgaaggaagg aagccagcga aggttgcggc ggcgggccgg
     1261 accggctgcg gaacggtcgc atcgcaacct tcgtccgggc ggcctgagcg gcgatgccac
     1321 ggtcggccga cgccaacacc ggacgacccg acgcccgccc cccgggcgcc gggacccacg
     1381 ccgccagcgc gacgaaaccc gtcgccggcg ccgggaccca cgccgccggc gacgccaacg
     1441 aggcggtccg ggccgagccc gccacgatcg ggaccggccg ggcgcgcgat ccgctcgccc
     1501 tcgagatccg cctcctcggc tcgctcctcg gccaggtgat cgccgagcag gcggggcgcg
     1561 acctcctcga cctcgtcgag cgggttcggc ggacgacgat ccggctccgg cgcggcgacg
     1621 acccagcgct ccgcgccgga ctcgtcgcgg agctggcggc gctggatccg gaccgggccg
     1681 aggtcgtcgt ccgcgccttc agcctctatt
//
LOCUS       DATAIW010000434         8974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_251734, whole genome shotgun sequence.
ACCESSION   DATAIW010000434 DATAIW010000000
VERSION     DATAIW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251734"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VHM48_12970"
     CDS             complement(<1..397)
                     /locus_tag="VHM48_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756374.1"
                     /translation="MTLDTIRGRLVLDDEVVVGALGIADGRIETVDIDRPAGAADTDQ
                     PYLAPGFVDVHVHGWGGHSAMGPTDDLAGMARALLRHGVTSFLPSAWTTPLPELHAVA
                     DRVRAWIPTAPSDGAEPLGFNLEGPFIAPA"
     gene            complement(394..1143)
                     /locus_tag="VHM48_12975"
     CDS             complement(394..1143)
                     /locus_tag="VHM48_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2756375.1"
                     /translation="MIDNGYIPHHRRIEQVLRERITAMQPGDRLPSDAELVAEFGVSR
                     MTARNAMQRLAEAGLVAREPGRGSFVATPATHRRTDRLMTFSSEMTRRGRVPSSRLLA
                     RAIRPSTRAEAAALALGPGEPVVEVRRIRLADNEPIALESAILVASCAPAVLNADLAH
                     GSLHGAITAGGQTLRRGTGTVAAGAATAEDARLLDIRRGDPLLIERRVIVDDDGRRIE
                     STESRYPADRYALDVSFDVEQTGPSIQEEAP"
     gene            complement(1140..2168)
                     /locus_tag="VHM48_12980"
     CDS             complement(1140..2168)
                     /locus_tag="VHM48_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2756376.1"
                     /translation="MATDSDAIPGSEPAATHHLGLDLGGTNLKWTVVEHAGADWRVLD
                     RGQVPTRSGGPDAIVPQLAEVGRAAIGQWPDVSTAGVGIPGLYDPRTGATRFLVNIPG
                     PWDGYPVAGPLADELGVPVALINDARAFGLAELRLGAGRGASSMVGLTLGTGVGGVIA
                     VDGKVHQGHDGTAGEVGHQTIDPDGPSCNCGNHGCLEAFCRADRIAEACGAATAEEAV
                     ERARSGDAAALEGLRRVGRYLGIGIGNMITVISPDRVVIGGGIAAAGDLLLDEARAEI
                     RRRVLTTSVDEVEIVTAELGTWAGAMGAAIHGAEQAPPQGTADASPGSAAVIAAIDAA
                     VDPASVVR"
     gene            complement(2172..3182)
                     /locus_tag="VHM48_12985"
     CDS             complement(2172..3182)
                     /locus_tag="VHM48_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756377.1"
                     /translation="MTPPAATAGNTGTPRSPSLESASWRRIVRDQPLIPLVVLLAVLV
                     ALLELAKPGIVSGNWVATTIRAAIPLAILAACQTLAMLTGGIDLSVGAVASMAAFLMA
                     TLATGQGPVVAIAVAFVAAAIAGLVNGIGIGMFKVHPLIMTLGMSLVVLGLMSVYQLL
                     MVQSGTLIPDPIKWLGSGISFGSVPNSLVVFAPVAVVIVIGLRRSGFGRLLFAVGDNP
                     IAARLAGTRVWQVLVVLYMLSGILAAIAGLLLAGLNNTATVSLVEQSVLPSVAAAVIG
                     GTSILGGRGGYAGTIVGALILTVLTTLLTVLEMPEAVRQVLFGAIIVAVAAAYTRVTG
                     EA"
     gene            complement(3179..4162)
                     /locus_tag="VHM48_12990"
     CDS             complement(3179..4162)
                     /locus_tag="VHM48_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756378.1"
                     /translation="MPRNRAEIVDLAGSWARRNVWTLGLLGLLVVFFAVTKLLQPNYG
                     PAAIQGLAIAILPIAFAAVAQAIVVISGGIDLSVGSMMALASVTSAVLMKGQGDLFGV
                     AVVVGVLLLGLLVGAINGGLVVATKVPDIVVTLAMSFVWAGTALLILNTPGGGAAPWL
                     MAIISGPLGTPWLPKAVVVLAVIVGVLWIPLRRSTLGLTLYALGSSPVAAFRSGVAVG
                     PTKIFAYALTGFFSAVGGLALTATGGVGAPVPGSYTLQSIAAIVLGGVSLAGGRGGVL
                     GPIIAVIVLALVRTDLFFAGVDPNWSTAIQGAILIGVVMFGSLVAMRRIRA"
     gene            complement(4180..5739)
                     /locus_tag="VHM48_12995"
     CDS             complement(4180..5739)
                     /locus_tag="VHM48_12995"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX2756379.1"
                     /translation="MIDGGPDLLLNATGVAKHYGAVVALRSASLAVRPGEVHALMGAN
                     GAGKSTLVKILTGGVRPDHGSIEVRGKPFAARSPTEARRGGIVSVYQEPSLIPDLDVR
                     SNLRLTRTPAEAVRHWLAELGLPDLDLASVVRDLPLASLRIIDLARALAIEPDILMLD
                     EMTAALPANLTERVLEVIGRMRGTDRSVIFISHRLIEIGAVCDRATVLREGETVGVVD
                     VTTGSEDRIVALMLGDIVRSDGTARATTPRAERPQDSVPRLTVRNLTVGSKLTDVSFD
                     LHPGEVLGVVALEGQGQDELFDVLSGSERPNGGDLLVDGGRASFRHPADAIRAGLVYV
                     PADRAEALLMQRSVRENIALPFTTPFGSWGLIDVRGERRKVDAAIATLQIDTRAAGEV
                     RRLSGGNQQKVTIARWVAGGVKTMLCFDPTRGIDIGTKQQIYQLLRDLAAAGAAVLLY
                     TSELKEIQLACDRAIVIFGGRIVDEIAVADADEATLLRAAYDLRPEELMPDEVASAAV
                     EAAEPSPAGLP"
     gene            complement(5831..6895)
                     /locus_tag="VHM48_13000"
     CDS             complement(5831..6895)
                     /locus_tag="VHM48_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2756380.1"
                     /translation="MKRLGLARGLVALAVTAIVAGACSSTPAASGGGAAKYTIGFSNA
                     GGVGNGWREAMLCSAKAQAVKAGNVSKVTIIHRDTDAPGQLADIRTLIAQGVNAIIIN
                     PSGPDALNPAIAEAIAANITVIAVDASVTAPGAYNLSNDQEQYAYLGASWLFKAMGDK
                     GAVVYMRGIAGHPADTDRDTGFQKALKEHPGITIAATTVTKWDPATATQQINDVMSAG
                     TKFDGIWTSGVDSNIVDALKAANHPYVPIVGADNAGFVTQLLAADGPKGAAVTNPASI
                     GGAGVTLALQILGGKKPAASTVHVTPVLWDNTTAEGKASLTAAADATLPKTWPLGLTI
                     QDWTTYTKPELIACKGPGDA"
     gene            complement(6988..7788)
                     /locus_tag="VHM48_13005"
     CDS             complement(6988..7788)
                     /locus_tag="VHM48_13005"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2756381.1"
                     /translation="MNVPTTVPALAWIAASGDILRRIAETQADAIERASSWCADAIAG
                     DGLVHLFGTGHSRIPVEEMFPRYGSYPGFNPIIELSMTFHTQVVGANGQRQAMFIERT
                     PGLAEVILSNFSFGHRDVMIVFSAGGLSAVPVEMARGARRRGLRVIAVTSVQQSRSSE
                     IDPIVGSRLLDEADLVIDLCSPPADAMIDIVGLDTPVGPGSTVTAVAIVNSIKVRTAQ
                     LLTELGAMPPVLTRSSVVGADRSRDLFDAAYREHARRLARAIDQRGGG"
     gene            complement(7785..8870)
                     /locus_tag="VHM48_13010"
     CDS             complement(7785..8870)
                     /locus_tag="VHM48_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2756382.1"
                     /translation="MRDVGIGMLGSGFIGEFHTLGLRHVPNARVVANCSAGAERREAF
                     ARRFDSRPYDSIDAVCADPDVDLVVVSLPNHLHLDAVRAATEHGKAVACTKPLGRTAA
                     EAAEMLRLASEAGVFHAYLENVVFTSETIRMHDMIEAGAIGRLVTFRAREGHSGPHAA
                     HFWDADLAGGGALLDMASHGAEVARYLFGKDVGVRDVFAWGATLVHGGRTSGEDNAVM
                     IVRFEDDRVATMDVSWSSKGGLENRFEVYGDAGRMVQDIGSTVLRAFIERPAGYIGEK
                     ADADTGWVFPVPDETHVHGHDAMMQDVVESFRAGSAPRETFHDGLAVNSIIDAAYRSM
                     RSGRWESVERAVAGGASGSAAAMVASR"
BASE COUNT         1340 a         3152 c         3227 g         1255 t
ORIGIN      
        1 gggccggggc gatgaacggc ccttcgaggt tgaagccgag cggctcggcg ccgtcggatg
       61 gcgcggtcgg gatccacgcc cggacccggt cggcgacggc atggagctcc ggcaggggcg
      121 tcgtccaggc gctcggcagg aacgaggtga cgccgtgccg gagcagggct cgcgccatgc
      181 ccgcgaggtc gtcggtcggg cccatcgccg aatggccgcc ccagccgtgg acgtggacgt
      241 cgacgaagcc cggcgcgagg tacggctgat ccgtgtccgc tgcgcccgcg ggtcgatcga
      301 tgtcgaccgt ctcgatccgg ccgtccgcga tccccagcgc cccgacgacg acctcgtcgt
      361 cgaggaccag ccgaccgcgg atcgtgtcga gcgtcatggc gcctcctcct gaatcgatgg
      421 gccggtctgc tcgacgtcga acgagacgtc gagggcgtaa cgatcggccg ggtagcgcga
      481 ttcggtcgac tcgatccggc gaccgtcgtc gtcgacgatc acccgtcgct cgatgagcag
      541 cgggtcgccg cggcgaatgt caagcagccg ggcgtcctcg gcggtggccg ccccggcggc
      601 gaccgtcccg gtgccgcgcc ggagggtctg gccgccggcc gtgatcgcgc catggagcga
      661 gccgtgcgcg aggtctgcgt tcaggacggc cggcgcgcag ctcgcgacga ggatcgcgga
      721 ttccagggcg atcggctcgt tgtcggccag gcggatccgg cgcacctcga cgaccggttc
      781 gcccggcccg agggcgaggg ccgcggcctc ggcccgggtc gatggccgga tcgcgcgagc
      841 gaggagccgc gagctcggaa cgcgcccgcg gcgggtcatc tcgctgctga acgtcatcag
      901 ccggtcggtc cggcggtggg tcgccggggt cgcgacaaag ctgccccggc cgggctcgcg
      961 cgcgaccagc cccgcctcgg ccaggcgctg catcgcgttc cgggcggtca tccggctgac
     1021 cccgaactcg gcgacgagct ccgcgtcgga cggcagccga tcgccgggct gcatggcggt
     1081 gatccgctcg cggagcacct gctcgatccg gcggtggtgg gggatgtagc cgttgtcgat
     1141 catcgaacga ccgatgccgg atcgacggcc gcatcgatgg cagcaatgac cgccgcgctg
     1201 ccggggcttg cgtccgccgt gccctgcggc ggcgcctgct cggcgccatg gatcgccgca
     1261 cccatcgccc cggcccacgt cccgagctcg gcggtgacga tctcgacctc gtcgaccgac
     1321 gtcgtgagga cccgccgccg gatctccgcc cgggcttcgt cgaggagcag gtcgccggcg
     1381 gccgcgatcc ctccgccgat cacgacccgg tccggggaga tgacggtgat catgttgccg
     1441 atcccgatcc cgaggtagcg accgacccgg cggagcccct cgagcgcggc cgcgtcgccg
     1501 ctccgggccc gttcgacggc ctcctccgcg gtcgccgccc cgcacgcctc ggcgatccga
     1561 tccgcccggc agaacgcctc gaggcagccg tgattgccgc agttgcacga cggcccgtcc
     1621 ggatcgatcg tctggtggcc gacctcgccg gccgtcccgt cgtggccctg gtggaccttg
     1681 ccgtcgaccg cgatcacgcc gccgaccccg gtcccgagcg tcagcccgac catcgacgac
     1741 gcgccgcgcc cggcgccgag ccggagctcg gccagcccga atgcccgggc atcgttgatc
     1801 agcgccacgg gcacgcccag ctcgtccgcg agcggaccgg ccacggggta gccgtcccac
     1861 gggccgggga tgttgacgag gaaccgcgtc gccccggttc gcgggtcgta gaggccgggg
     1921 atcccgaccc ccgccgtcga gacgtccggc cattgcccga tcgcggctcg gccgacctcc
     1981 gcgagctggg ggacgatcgc gtcggggcca ccggaccggg tcgggacctg gccgcgatcc
     2041 agcacccgcc agtccgcccc ggcgtgctcg acgacggtcc acttgagatt cgtgcccccg
     2101 aggtccaacc cgagatggtg ggtcgccgct ggctccgatc ccgggatcgc gtctgagtcg
     2161 gtggccatcg atcaggcttc cccggtgacc cgggtatagg ccgccgcgac cgccacgatg
     2221 atcgccccga acaggacctg gcggacggcc tcgggcatct cgagcaccgt cagcagcgtc
     2281 gtcaggacgg tcaggatcag cgctccgacg atcgtcccgg cgtagccacc gcgcccgccc
     2341 aggatcgacg tgccgccgat gaccgcggcg gccaccgacg gcaggaccga ctgctcgacg
     2401 aggctcaccg tggctgtgtt gttgaggccg gcgaggagga gtccggcgat cgcggccagg
     2461 atccccgaca gcatgtacag gacgacgagc acctgccaga cccgtgtccc ggccaggcgt
     2521 gcggcgatcg ggttgtcgcc gaccgcgaac agcaaccgac cgaagccgct ccgccgcagg
     2581 ccgatgacga tcacgacggc gaccggggcg aagacgacga ggctgttggg cacggagccg
     2641 aacgagatgc cggagccgag ccacttgatc gggtcgggga tgagggtccc cgactggacc
     2701 atcaggagct ggtacaccga catcagcccg aggacgacga ggctcatgcc cagcgtcatg
     2761 atcaggggat ggaccttgaa catgccgatg ccgatcccgt tgacgaggcc ggcgatcgcg
     2821 gcggcgacga aggcgaccgc gatcgccacc actggaccct ggccggtcgc gagggttgcc
     2881 atgaggaacg ccgccatcga ggcaaccgcg ccgacggaca agtcgattcc gccggtgagc
     2941 atcgccagcg tctggcacgc ggcgaggatc gcgagcggga tcgcggcacg gatcgtcgtg
     3001 gcgacccagt tgccgctcac gatcccgggc ttcgccagct cgaggagcgc gacgaggacg
     3061 gcgaggagga cgacgagggg aatgagcggc tgatcgcgga cgatccggcg ccaactggcg
     3121 ctctccagag acgggctgcg cggggtgccg gtgttcccgg ccgtggcagc aggcggtgtc
     3181 atgcccggat cctccgcatc gcgaccaggc tgccgaacat cacgaccccg atgaggatcg
     3241 cgccctggat cgccgtcgac cagttggggt cgacgccggc gaagaacagg tcggtccgca
     3301 ccagggccag gacgatcacc gcgatgatcg gtcccagcac gccgccgcgt ccgccggcga
     3361 ggctgacccc gccgagcacg atcgcggcga tgctctggag cgtgtaggag cccggcacgg
     3421 gcgccccgac cccgcccgtg gcggtgaggg ccagcccgcc cacggccgag aagaaccccg
     3481 tcagggcgta ggcgaagatc ttcgtcgggc cgaccgcgac gccactccgg aacgccgcca
     3541 cgggactgct cccgagcgca tacagggtca gccccagggt cgagcgccgg agcgggatcc
     3601 acaggacgcc gacgatcacc gcgagcacga ccacggcctt cggcaaccac ggggtcccga
     3661 ggggtcccga gatgatcgcc atgagccacg gtgccgctcc gccgcccggg gtgttgagga
     3721 tcagcagcgc cgttcctgcc cagacgaagg acatcgcgag cgtgaccacg atgtccggca
     3781 ccttcgtggc cacgacgaga ccgccgttga tcgctccgac caacagcccg agcaggagga
     3841 cacccacgac gaccgccacg ccgaagaggt cgccctggcc cttcatcagg accgccgagg
     3901 tcacgctggc gagcgccatc atcgatccga ccgacaggtc gatgccgccg gagatcacca
     3961 cgatcgcctg cgccaccgcg gcgaacgcga tcggcaggat cgcgatcgcg aggccctgga
     4021 tcgcggctgg tccgtagttc ggctggagaa gcttcgtgac cgcgaagaag acgacgagga
     4081 ggccgagcag gccgagggtc cacacgttgc gccgcgccca cgagccggca agatcgacga
     4141 tctcggcgcg gttgcgcggc acgctggtcg ctccgctgct cacggcagac ccgccgggct
     4201 cggctccgcc gcctcgaccg ccgcggacgc gacctcgtcc ggcatgagct cttcgggccg
     4261 gaggtcgtag gcagctcgga gcagcgtcgc ctcgtcggca tccgcgaccg cgatctcgtc
     4321 cacgatccgc ccgccgaaga tcacgatcgc ccgatcgcag gcgagctgga tctccttgag
     4381 ctcggacgtg tagagcagga ccgccgcgcc cgcggccgcg aggtcgcgga ggagctggta
     4441 gatctgctgc ttggtcccga tgtcgatgcc gcgggtcgga tcgaagcaga gcatcgtctt
     4501 gaccccgccg gccacccacc gggcgatcgt gaccttctgc tggttgccgc cggacaggcg
     4561 gcgcacctcg ccggcggcgc gagtgtcgat ctgcaacgtc gcgatcgcgg cgtcgacctt
     4621 gcgccgctcg ccgcggacgt cgatcaggcc ccacgagccg aacggcgtgg tgaacggcaa
     4681 ggcgatgttc tcgcgcaccg agcgttgcat caggagcgcc tcggcgcgat cggctggaac
     4741 gtagacgagg cccgctcgga tcgcgtcggc cggatgacgg aacgaggcac gcccaccgtc
     4801 gacgagcagg tcgccaccgt tcggccgctc cgaacccgac aggacgtcga acagctcgtc
     4861 ctggccctgg ccctcgagcg cgacgacgcc gaggacctcg ccaggatgga gatcgaacga
     4921 gacgtcggtc agcttcgagc cgacggtcag gttgcggacc gtcagtcggg ggacggaatc
     4981 ctgcgggcgc tcggcccggg gggtcgtggc tcgcgcggtc ccgtcgctgc ggacgatgtc
     5041 gcccagcatc aacgcgacga tccgatcctc cgacccggtc gtgacatcga cgacgccgac
     5101 cgtctcgccc tcgcgcaaca cggtcgcccg gtcgcagacg gcgccgatct cgatcagccg
     5161 gtgggagatg aagatgaccg accggtcggt gccgcgcatc cggccgatga cctcgaggac
     5221 gcgctcggtc aggttcgcgg gcaacgcggc ggtcatctcg tcgagcatca ggatgtccgg
     5281 ctcgatggcc agcgcccggg cgaggtcgat gatccggagc gaagcgagcg gcaggtcccg
     5341 gacgacgctg gcgagatcca ggtcgggcag gcccagctcc gcgagccaat ggcggacggc
     5401 ctcggcgggc gtacgcgtca gccgcaggtt ggaccggacg tcgaggtccg ggatcagcga
     5461 cggctcctgg tagaccgaca cgatcccgcc acggcgggcc tctgtcgggg agcgcgccgc
     5521 gaagggtttg ccacggacct cgatcgaccc gtggtcgggg cggacgccgc cggtgaggat
     5581 cttgacgagc gtgctcttgc cggcgccgtt cgcgcccatg agggcatgaa cctcgcccgg
     5641 ccggaccgcg agagatgcgg atcgcagcgc gacgaccgcc ccgtagtgct ttgcgacacc
     5701 ggtggcgttc agcaggaggt caggtccgcc gtcgatcacg tggtggttcg tcctggtcgg
     5761 tgggttggat tggaaacggg ggtgccggca gagccggcac ccccacgctc agtcgcgtta
     5821 cggagtcggg ctacgcgtcg cccggaccct tgcaggcgat cagttcgggc ttggtgtaag
     5881 tggtccaatc ctggatcgtc agaccgagtg gccaggtctt ggggagggtg gcgtcggcgg
     5941 cggcggtcag agacgccttg ccctcggccg tcgtgttgtc ccacaggacc ggagtcacgt
     6001 ggaccgtcga tgccgcaggc ttcttgccgc cgaggatctg gagggcgagg gtgacaccgg
     6061 cgccgccgat cgaggccggg ttggtgaccg cggcgccctt cggcccatcg gccgccagca
     6121 gctgggtgac gaagcccgcg ttgtccgcgc cgacgatcgg cacgtacggg tggttggcgg
     6181 ccttgagcgc gtcgacgatg ttcgagtcga cgcccgacgt ccagatgccg tcgaacttcg
     6241 tgccggcgct catcacgtca ttgatctgct gggtcgcggt cgccgggtcc cacttggtga
     6301 ccgtcgtagc cgcgatcgtg atgcccggat gctccttgag cgccttctgg aagcccgtgt
     6361 cgcgatccgt gtcggccgga tggccggcga tgccgcgcat gtagacgacg gcgcccttgt
     6421 cgcccatcgc cttgaacagc cacgaggcgc cgaggtaggc gtactgctcc tggtcattgg
     6481 agaggttgta ggcgcccggt gccgtgaccg aggcgtcgac cgcgatgacc gtgatgttgg
     6541 cggcgatcgc ctcggcgatc gcggggttca gggcgtccgg cccggacggg ttgatgatga
     6601 tcgcgttgac gccctgggcg atcagcgtcc ggatgtcggc cagctggccg ggagcatccg
     6661 tgtcgcggtg gatgatcgtg accttggaga cgttgccggc cttgacggcc tgcgccttcg
     6721 ccgagcagag catcgcctcg cgccagccgt tgccgacgcc gccggcgttc gagaatccga
     6781 tcgtgtactt cgcggcgccg ccgccggacg ccgccggcgt cgagctgcag gcaccggcga
     6841 cgatcgccgt gacggccagg gccacgaggc cgcgagccaa accgagtcgt ttcatggacg
     6901 ttccactcct ccttgcgccg accgcggtcc ccgcgtgggt cgtcagtcgg accatcatcg
     6961 ctggattgcg tgccgctcgt cgctccgtca cccacctcct cgttggtcga tcgcacgggc
     7021 gagccgccgc gcatgctctc gataggctgc atcgaacagg tcccgggacc ggtccgcccc
     7081 gaccacgctg ctgcgcgtca ggacgggtgg catcgcgccc agctcggtga ggagctgggc
     7141 ggtccggacc ttgatggaat tcacgatcgc gaccgcggtg acggtcgagc caggtccgac
     7201 cggggtgtcg agcccgacga tgtcgatcat cgcgtcggcc ggcggcgagc agagatcgat
     7261 gacgaggtcg gcctcgtcga gaaggcggct gccgacgatc gggtcgatct cggacgaccg
     7321 cgactgctga acggaggtga cggcgatcac tcgcaggccg cgccgccgcg ccccacgggc
     7381 catctcgacc gggacggccg acaggccgcc cgcggagaag acgatcatca cgtccctgtg
     7441 gccgaacgag aagttcgaca ggatcacctc ggccagcccc ggcgttcgct cgatgaacat
     7501 ggcctggcgc tgtccgttgg cgccgacgac ctgggtgtgg aaggtcatcg acagctcgat
     7561 gatcgggttg aagcccggat acgagccgta ccgcgggaac atctcctcga ccgggatccg
     7621 cgagtggccg gtcccgaaca ggtggaccag gccgtcgccg gcgatcgcgt cggcgcacca
     7681 tgagctggcc cgctcgatcg catcggcctg cgtctcggcg atccgccgga ggatgtcgcc
     7741 cgaggccgcg atccaggcga gcgccggcac cgtggtcggg acgttcatcg ggaggccacc
     7801 atcgcggcgg cgctgccgga tgccccgccg gccacggccc gctcgaccga ttcccagcga
     7861 ccgctgcgca tggagcggta cgccgcgtcg atgatcgagt tcacggccag gccgtcgtgg
     7921 aacgtctcgc gaggcgcgct gccggcgcgg aatgactcga ccacgtcctg catcatcgcg
     7981 tcgtggccgt ggacatgggt ctcgtccggg acgggaaaga cccagcccgt gtcggcatcg
     8041 gccttctcgc cgatgtatcc ggcgggacgc tcgatgaacg cccgcaggac ggtcgagccg
     8101 atgtcctgga ccatccggcc ggcgtcgccg tagacctcga agcggttctc gaggccgcct
     8161 ttcgacgacc acgagacgtc catcgtcgcg actcgatcgt cctcgaagcg gacgatcatg
     8221 acggcgttgt cctcgccgct ggtccgccct ccgtgcacca gcgtcgcgcc ccaggcgaag
     8281 acgtcgcgga cgccgacatc cttgccgaag aggtagcgag cgacttcggc gccgtgggac
     8341 gccatgtcga gcagcgcgcc accgccggcc agatccgcgt cccagaagtg cgcggcgtgc
     8401 ggcccgctgt ggccctcgcg agcgcggaac gtgaccagcc ggccgatggc tcccgcctcg
     8461 atcatgtcgt gcatccggat cgtttcgctg gtgaagacga cgttctcgag atacgcgtgg
     8521 aagacgcccg cttcgctggc caggcgcagc atctcggcgg cctccgctgc ggttcgaccg
     8581 agcggcttgg tgcaggcaac ggccttgccg tgctcggtcg cggcccgcac tgcatccagg
     8641 tgcaggtggt tcggcagcga gacgacgacg aggtcgacgt cgggatccgc gcagacggcg
     8701 tcgatcgagt cgtacggccg gctgtcgaac cgacgcgcga aggcctcgcg tcgctcggcg
     8761 ccggcactgc agttcgcgac cacgcgggcg ttgggaacgt ggcgcaggcc gagcgtatgg
     8821 aactcgccga tgaagcctga gccgagcatc ccgatcccga cgtcacgcac ggtcagcgtc
     8881 cagcctccga cagcgcgccc gccgaaacgg gctgtgcgac cgtgtcgcat ggccggacgt
     8941 cgccacatga tccaacccgg gtcgtacccg gggt
//
LOCUS       DATAIW010000435        19236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_281813, whole genome shotgun sequence.
ACCESSION   DATAIW010000435 DATAIW010000000
VERSION     DATAIW010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281813"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1730
                     /locus_tag="VHM48_13015"
     CDS             321..1730
                     /locus_tag="VHM48_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756383.1"
                     /translation="MKPVRPTSASPWTRMLNRRDILKLGAVLAGGGAVAPLLTACSGP
                     TGSRAAPVGTSAGGSPPPLSGPITLLTEGGDPTTQPTLKKVYDGFAAQNPGIDWDIRA
                     PAGASPEWDRLARALLESGEPVGLILIDGQFVRAWARDGLLADLAADPAMADVLARVP
                     RRFQFPGPGGTIWAFPLAASMGVQTTGLYYNKAILDRAGLEAPRTLADLTAMVKPLAA
                     MGVAPLVHCSGDAVFNPLLVTWLLPMIAEGTGDPLAFAESTIRGDIRYDSPEWIEAFR
                     IIADLRTSGVLLRGSGATDYGTMQQLMLQGKAAMTCNGSWMLTQILAGTPSGAFDLHV
                     APLPLVPGATKARPILAWGGFALPAQAARSRDSVHAFLEYASRPEVDRAVVAGLQAYS
                     SIAASNDAIQNAVAQEFLPMFEDAITPMDWLWEPEITTEVGNQVQALVKGDTDPAAVG
                     KAIQAVAEELRSSGRSYYP"
     gene            1736..4486
                     /locus_tag="VHM48_13020"
     CDS             1736..4486
                     /locus_tag="VHM48_13020"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2756384.1"
                     /translation="MTSLLLDTKLYAPRRRSGLVPRPQLTERLRRGTESKLTLVSAPA
                     GFGKSTLLAEWLAIAAADGSVTAWLSLDPGDNHPVMFWTHLIAALQTAAPGVGASASS
                     LLGSPDPPIEAALASLLNELNSVPNDIVLVLDDYHTIDEQQIQAGMTYVLEHLPDQVH
                     LAIATRVDPALPLARLRARGELAEIRAVDLRFTPDEAAAYFNGVMNLGLTARDVAALE
                     GRTEGWIAALQLAGLSMQGRDDITGFITGFAGDDRYIVDYLVEEVLQREPEDVRSFLL
                     QTSILARMSGPLCDAVTGRDGGKAMLEVLDRRNLFLIPLDDRRRWYRYHHLFADVLQA
                     HLLDELPDLAADLHRRATEWFEQNGERSEAIRHAMAGKDFARAADLVELAMPATGRDR
                     QEATLRRWLEALPDELIRARPVLSNGYAGSILVRGETDGVEARLRDAEGWLDGAAEAP
                     DEVGVRPASMVVADEDAFRRLPGMIAIHRAGQARLLGDVAGTIAHARRALDLFDDDDQ
                     LGQGGASALLGLAYWTSADLEAASGLYAGAMPRFEKAGHLSDAIGLALALADMRIAQG
                     RLREAMSTYERGLELATGQGALALRGAADMHVGISEILRERDDLMGAAQHLHQARDLG
                     DENGLPQNPYRSRVAAARIRQAEGDLDGSLQLLDEAERLYANDFSPDVRPVAALKARA
                     RIAQGKLSEASAWAGDRGLSSADDLSYVREFEYVTLARLLLAQGSRDRSDQLVAEATD
                     LLERLLVAAESGGRTGTVIEILVVLALARHARDDVTGALASLGRAVALAEPEGYVRIV
                     VDEGRPIAALLKLAAKQPTTPTYVRRLLAAAVPADGRTPIEQSLIEPLSERELEVLRL
                     LDSDLDGPDVARELTLSLATVRTHTRNIYAKLGVNNRRAAVRRADELGLLSRTRDRRP
                     SV"
     gene            4657..4932
                     /locus_tag="VHM48_13025"
     CDS             4657..4932
                     /locus_tag="VHM48_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756385.1"
                     /translation="MTDQRTPTPDRPEAGRYEIRLTGHLDAHWTTWFDGLSVSREVDG
                     TTVISGPIADQSALHGVLQRVRDLGLPLVSVIRMKPDQPETTGINPG"
     gene            4960..5556
                     /locus_tag="VHM48_13030"
     CDS             4960..5556
                     /locus_tag="VHM48_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756386.1"
                     /translation="MITTRIQTMTRLFMATVSLAAVAVALVAAPAAGAAGLRNCVDVT
                     GPQSGRAGCYEDVWANGAQYRMTFSNTRFTGATPKALDPFYVIAPQTDRPQGAPPNTF
                     AHDHVVRNVPHQNHGEYSVKMQGFFVLCSGQGIVSGACVPSWTSIGGPDPLPFAKTVD
                     GQPLTSTDAIEAAAATGNLALINLGPGAVIIGAVTGSN"
     gene            5637..6440
                     /locus_tag="VHM48_13035"
     CDS             5637..6440
                     /locus_tag="VHM48_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX2756387.1"
                     /translation="MTNIQRTTTAKRTPMDASRKVALAVGVLFILTFVTSIAGVFAYG
                     PVLSDPSYITGAGPDTRVFVGAFLELMLILTNIGCATVLFPLLKRQNEGLALGYVAAR
                     IVECTFILVGILSVLAIVTLRQTATAADAGSILTIGKALHAIHDWTFLFGPGFTDGIG
                     TGLILGWLMYRSGLVSRRMALFGVVGGPLLAASGIAVLLGVIPQGSAAQGIATIPEIV
                     WEMFLGLWLTFKGFKATAPAAVAFDSDAAEQSATAAAGPIGVATKAGAA"
     gene            6437..6931
                     /locus_tag="VHM48_13040"
     CDS             6437..6931
                     /locus_tag="VHM48_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2756388.1"
                     /translation="MTAVREQAPRTMPRFVLRIFWMLHRGIDRLTGGRFGLSRPEAGR
                     KFGMLRLATVGRRSGQPRIAIVGYYEDGPNLVTLAMNGWGRSEPAWWLNLQARPDTTV
                     GLADGPRAVRARAATGAERELLWARFADFPGWGADIDALAARRPDETTVVVFEPRDDA
                     RIVA"
     gene            6928..7320
                     /locus_tag="VHM48_13045"
     CDS             6928..7320
                     /locus_tag="VHM48_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HEX2756389.1"
                     /translation="MTATIVARPRRPAIRHGWLVPGLALAVYANTLAADHGLGLVPVL
                     LFGIAPHLTVLVGFGQPHARGQLAPRAVPLFDTMHHPAVPLAVLGLAAAGVLPPFWFV
                     AALAWLGHIAVDRGLGDGLRTADGWQRR"
     gene            7588..8634
                     /locus_tag="VHM48_13050"
     CDS             7588..8634
                     /locus_tag="VHM48_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2756390.1"
                     /translation="MAETESGKRVTLADVAAAAGVSPAAVSLAVRGEPGVSEETRRRI
                     VETARRLGYRTVNRWGSRRSRQTTIGLVIKAPQGDAPKANRFYAPVMAGIEESCRLRS
                     VDLLLATMPVDADYHPIEVPRLVAERATDGLIVVGAHLSRGTTELLEDGPPVLLVDAY
                     AEDAAFDSVVSDDVGGARAAVEHLIAKGHRAIAIVGSHPGSFPSILQRRRGYEQAMTE
                     AGLQPHFADGPHVPPEVGAAAGLDYVCAHPEITAVFCCNDDVAVAFSQSAQRTGIVVP
                     DAMSVVGYDDIDLAAFVTPRLTTMAVDKIGMGHLAVTLLLHRLELGSAAVTQTLIRPR
                     LVERESVATRLPSS"
     gene            complement(8631..9734)
                     /locus_tag="VHM48_13055"
     CDS             complement(8631..9734)
                     /locus_tag="VHM48_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX2756391.1"
                     /translation="MPESQPSTPGTAVTADDRPAPLDMDDAAATDGVLTPQLAPRTSL
                     PGAAARAPIPWEDRPAAENGVLWRSSRNPIIRRDHLPRSNSIFNSAVVPHGGGFAGIF
                     RVDDRSREMNLHAGRSRDGIDWDIEPEPIRFHAADGRVAEIQAAFEHAYDPRVTWLED
                     RYYVTWCSGYHGPTIGIGYTHDFETFHQLDNAFLPFNRNGVLFPRRVGAKYAMLSRPS
                     DNGHTPFGDIFVSESPDLVHWGRHRHVLAPEPWTWQSTKIGAGPTPIETSEGWLLIYH
                     GVLTSANGFVYSMGAALLDLERPWQVIARGRDYLLSPQVSYEQVGDVPNVVFPCAAVL
                     DSDGERITIYYGAADTVVCIAHGYLSEILASIR"
     gene            complement(10003..10278)
                     /locus_tag="VHM48_13060"
     CDS             complement(10003..10278)
                     /locus_tag="VHM48_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756392.1"
                     /translation="MNTLSTMSVRFVRTVISPDTVATDGATDAGWAEPVTGAEATELA
                     LGLGVALPEQAATDTNNNVARIAEIRFPIPISQWRGRGGHAAVHGRT"
     gene            10271..11434
                     /locus_tag="VHM48_13065"
     CDS             10271..11434
                     /locus_tag="VHM48_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756393.1"
                     /translation="MFTAKYLPLFNAKYPGVKVKFEALTDYEGETKTRMNTTEYGDVL
                     LIPNAITPDKLAEFFEPLGTLDELSQKYNFTTEQLSNGKVYGLAVTGNANGFVYNKKV
                     WDAAGVTSLPKTPDEFLADLKLIKDKESSVTPYYTNYKDGWPLTQWQSNRGQITNDPN
                     YANGTLVHADAPWAAGTDENVIYSLLYNIVKQGLSEKDPTTTAWEPSKALLGGGKVAT
                     MQLGSWAIVQMQEAAVKAGGSADDIHYLPFPNQVGGKFISNIGGDYKIGINVHSQNKE
                     AARAWIDWFENESNFAFDQGGVSPVKGGKNPPQLSDFDGVGVAYIEQAPAAAGEEGLL
                     GKIQDQSEIQLFDQAWPQRIVDAARGATNEGLDAIFADLNGKWKAARATLSIK"
     gene            11583..12512
                     /locus_tag="VHM48_13070"
     CDS             11583..12512
                     /locus_tag="VHM48_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2756394.1"
                     /translation="MTTVNLTKATARTSRAATPGPGHRRPWTPFLFLVVPVGLLLLLT
                     YLPLLFMVQFSVFRWDGFSSDRTFVGPQNYIDIFTRPELFGVFFVSLYYFAASFVQIG
                     VALYLATLLSFKVRFKNVFKGIIFFPYLINGVAIALIFTYFFRPDGVLDSLLAIGGVN
                     DPPLWLGDRRLVNISLAGVSVWRYMGLNFVLFLGAIQSIPSEMLEAGEIDGASRWQRF
                     RYLIAPGIRPVIALSFILAISGSLAVFEIPFIMLFGANGSATFVIQTVQTAFTFHKFG
                     LAAAMAAVLLGLVLLVTLVQRRLLPDDPVELTV"
     gene            12509..13354
                     /locus_tag="VHM48_13075"
     CDS             12509..13354
                     /locus_tag="VHM48_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2756395.1"
                     /translation="MTEPSTRLRVWLGRTITYGSLGLACLVALLPLVVLMMASLKTSN
                     EFATQGVLDPPGDWFNFSNFAAFFVEGNVVQGFLNTSFILVISVIGTVIIGSMTAFAL
                     DRFEFRLKRTVVLLFLFAALVPTVTAQVTTFQLVANMHLYNTRFAPILLFIGTDIVSI
                     YIFMQFMRGIPRSLDEVALLDGASQFTIYRKIVLPLMKPAIATVVIIKGVAIYNEFYI
                     PYLYMPSRDLGVVATTLSRFAGPQSTRWEVIAAGVVITMIPTLVAFLVLQRYIYNGFT
                     RGAAK"
     gene            complement(13395..14366)
                     /locus_tag="VHM48_13080"
     CDS             complement(13395..14366)
                     /locus_tag="VHM48_13080"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX2756396.1"
                     /translation="MPQSDLPIAVLRSYAPDLAVPSDLEAFWSTTLADARGHDLGATF
                     EPMDNHLAVISTLDVTYAGFGGSPVRAWLHLPVGHDAPLPGVVEYVGYGGGRGLAHER
                     ILWAAAGYAHLVMDTRGQGSAWAVGETADPDAGGAPFHPGFMTQGILDPRTYYFRRVF
                     TDAVRAIDAIRMHPAVDPGRVAVTGGSQGGGISLAVAALVPDVAAAMPDVPFLCDFPR
                     AITFADSDPYGEIVRYLKAHRDHVELALATLSYFDAAILGRRATAPALFSVGLMDEIS
                     PPSTVYAAYNAYGGPKEIHEYPFNDHEGGQGFHDVARLRWLGELLRA"
     gene            complement(14376..15374)
                     /locus_tag="VHM48_13085"
     CDS             complement(14376..15374)
                     /locus_tag="VHM48_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX2756397.1"
                     /translation="MAWYGFNMQWMLVGGGRRPLPPDERALDFLSGLDFNFVRMPTDY
                     RTWTTGFDYGHPDESVFATLDDHLVACRLRGIHLSLNLHRAPGYCITRNDLERDNLWL
                     DRVAQDGFVGLWEGFARRYLGTAAEALSFDLLNEPPAIGLDGFTRDRHAALIRRTVAA
                     IRAIDPDRPIVIDGLDGGNLAMPELADLGIIHGGRGYQPYPVSHWGAEWWDGWKGGDA
                     PRYPGVEFEGTTWGPAALRAFYEPWRAVERTGVGVHIGELGCYNRTPNDDAMRWFRDL
                     FGLFREFGWGWAMWNLEGPFGIVGHGRPGARIEMRHGYPVDVDLLELMLSSRISQE"
     gene            complement(15907..16332)
                     /locus_tag="VHM48_13090"
     CDS             complement(15907..16332)
                     /locus_tag="VHM48_13090"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2756398.1"
                     /translation="MTDWDPEAFTRNLIADMRAHGGRASAGPMAGQPLMILTTTGAKS
                     GEPRQAIVTYHADGDRWAIAGSKGGAPTHPAWFHNLEAHPTATIEVANETVPVRALIA
                     DGEERERLWNDHVAALPAFGEYPAKTDRVIPMILLERVD"
     gene            complement(16419..17429)
                     /locus_tag="VHM48_13095"
     CDS             complement(16419..17429)
                     /locus_tag="VHM48_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2756399.1"
                     /translation="MVALRELEPDDAAAAAAADDALTAAIEDEALDAYSSAVSTAAAR
                     LIPSVASLRVDRRNGGWGGGSGSAVAFTADGFLVTNAHVVAGAERGTATFVDGDELSF
                     EVVGRDPLSDLAVVRSSGADLAAAPFGDAARLRVGQLVVAIGNPLGFAGSVTAGVVSA
                     LGRSLAARDGQASRLVENVIQTDAALNPGNSGGALADVRGRVVGINTAVAGVGLGLAV
                     PIDAATRLILAALMRDGRVRRAYLGIVGGTRRLPAALADRLGRAAGLEVVQLLDGSPA
                     AAAGVRAGDLIVSLDGRPIAGVGDLQRSLVGDLVGRSIEVTLERDGILAELALTPAEL
                     RT"
     gene            complement(17435..18331)
                     /locus_tag="VHM48_13100"
     CDS             complement(17435..18331)
                     /locus_tag="VHM48_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2756400.1"
                     /translation="MSILDDLSSAIRSVHESAGPAVVGIGQRNRGSGVVIGDGRVLTN
                     AHNLRGDEVTVTFRNGRSERGTVRGVDPDGDLAVIEVDTTGATTLAWSDVETTIGDVV
                     FGLASTGAGAARVTAGTISAIEQTFRGPGGSRIGGSLEHTAPLASGSSGGPLVDGSGR
                     LVALNTNRIGEGFYLARPADAAFRVRVDALSRGESVSRPRLGIAVAPSVVAQRLRRSV
                     GLAERDGLLVRGVEEGSPADRAGIREGDLLVEAAGKPLTDVDSLPAILAEAGMPIALK
                     IVRGDEERTVSVGGTTDASGEA"
     gene            18483..18926
                     /locus_tag="VHM48_13105"
     CDS             18483..18926
                     /locus_tag="VHM48_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2756401.1"
                     /translation="MTEPATAPPSPTALESALDRVGDRWSLLIVDALLDGPRRFGELR
                     DALPGIAPNILTDRLRRLERERILGSTPYSTRPPRMEYRLTGDGRDLASALRLLADWG
                     SRRTGGEPLRHDRCGTAIEARWFCPTCAVAVGDAEISGAEETRLV"
     gene            complement(18946..19101)
                     /locus_tag="VHM48_13110"
     CDS             complement(18946..19101)
                     /locus_tag="VHM48_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756402.1"
                     /translation="MGIDVGRTVTYQDGKVLHRTTYYSHYSRVTGVLLIGRGRRPGRS
                     GLTGRPT"
BASE COUNT         2927 a         6783 c         6559 g         2967 t
ORIGIN      
        1 ctgagcacaa cgccgtacac gagcggatac aggaagaacc cggccaggat cgccagaatt
       61 ggcaggagga acagcaggtc gatcgcgcgt tcgcgccgtg agccgaggac catcaaacgg
      121 gtcttcgatc cggtcgtcgt ccgcctatgg cgatcgatcg gcaggcgccc atcaccggac
      181 gatcaacgac cgacggacgc ggtgtgccca gccacgctcg aacgatagac aatgttcgtc
      241 cggctgacgc acatttcctg cgcgggccgc acagcagttc tcccgcggtc cacgctgccc
      301 gctgtcaccg aggaacgccg atgaagcccg ttcgtccgac gagcgccagc ccgtggacgc
      361 ggatgctgaa tcgccgcgac atcctgaagc tcggagccgt ccttgccggc ggcggagccg
      421 tcgcgccgct cctcacagcc tgctccgggc cgaccggaag ccgggccgcg cccgtcggga
      481 cgagcgcagg cggcagccca ccgcccctgt ccggtccgat caccctcctg accgaaggcg
      541 gcgatccgac gacccagccc acgctcaaga aggtctacga cggcttcgcg gcccagaacc
      601 cgggtatcga ctgggacatc cgggcgcctg cgggcgccag ccccgaatgg gatcggctcg
      661 cgcgcgccct gctggagtcc ggcgagccgg taggtctgat cctgatcgac gggcagttcg
      721 tccgggcatg ggcgcgagac ggcctcctgg cggacctcgc cgccgacccc gcgatggccg
      781 acgtgctggc ccgcgtaccc aggcggttcc agttcccggg tccgggcgga accatctggg
      841 ccttcccgct ggctgcgagc atgggcgtcc agaccacggg cctctactac aacaaggcga
      901 tcctcgaccg ggccggactc gaggctccca ggacgctcgc ggacctcacg gcgatggtga
      961 agccgctggc ggccatgggc gtcgcgccgc tcgtccattg ctccggtgac gccgtcttca
     1021 atccattgct cgtcacgtgg ctgctgccga tgatcgcgga agggaccggc gacccactgg
     1081 ccttcgccga gagcacgatc cggggcgaca tccgctacga cagccccgaa tggatcgagg
     1141 cgttccggat catcgccgac ctgcggacgt cgggcgtcct gctgcgcggc tcaggcgcca
     1201 cggactacgg gacgatgcag caactcatgc ttcagggcaa ggcggccatg acctgcaacg
     1261 gctcgtggat gctgacgcag atcctggcag gcacgcccag cggtgcgttc gacctgcacg
     1321 tcgcgccgct ccccctggtg cccggcgcga cgaaggcgcg cccgatcctg gcgtggggcg
     1381 gattcgcgct gccggcgcag gccgcccgca gccgggacag cgtgcatgct ttcctcgagt
     1441 acgccagccg tccggaggtc gaccgagccg tggtcgcggg cctccaggcg tactcgtcga
     1501 tcgcggcctc gaacgacgcg atccagaacg ccgtcgcgca ggagttcctg ccgatgttcg
     1561 aggatgcgat cacgccgatg gactggctct gggagcccga gatcaccacc gaggtcggca
     1621 accaggtcca ggccctcgtg aagggcgaca cggacccggc agctgtcggg aaggccatcc
     1681 aggcggtcgc cgaggaactc cgctcgtccg ggcgcagcta ctacccttga ggggcgtgac
     1741 cagcctgctg ctcgacacca agctctatgc cccgaggcgg cggagcggcc tcgtcccccg
     1801 gccgcaattg accgagcgct tgcgacgcgg gaccgagtcc aagctgacgc tcgtctcggc
     1861 cccggccggc ttcggcaagt cgacgctcct cgccgaatgg ctggcgatcg ctgcggccga
     1921 tggatcggtc acggcgtggc tctcgctcga tcccggcgac aaccaccccg tcatgttctg
     1981 gacccacctg atcgccgcgc tgcagacggc agcgcccggg gtcggggcga gcgcgagctc
     2041 gctcctgggg tcacccgacc cgcccatcga agccgccctg gcttccctgc tcaacgagct
     2101 caactcggtg ccgaacgaca tcgtgctggt gctcgacgac taccacacga tcgacgagca
     2161 gcagatccag gccggcatga cgtatgtgct cgagcacctg ccggaccagg tccacctggc
     2221 gatcgccacc cgcgtcgatc cggcgctgcc gttggcccgc ctccgggcgc gcggtgagct
     2281 tgccgagatc cgtgccgtcg atctccgttt cacgcccgac gaggccgcag cgtatttcaa
     2341 cggggtgatg aacctggggc tgaccgcgcg cgatgtcgca gcgttggagg gtcggaccga
     2401 aggctggatc gccgccctcc agctggcggg actctcgatg caggggcgag acgacatcac
     2461 cggtttcatc accggtttcg ccggcgacga tcgctacatc gtcgattacc tcgtcgaaga
     2521 ggtcctgcaa cgtgaacccg aggatgtccg gagcttcctg ctgcagacgt cgatcctggc
     2581 ccgcatgagc ggtccgttgt gcgatgccgt caccggtcgg gacggtggca aggccatgct
     2641 ggaggtcctc gatcgacgga acctgttcct gatcccgctc gacgaccgcc gacgttggta
     2701 tcggtaccac cacctgtttg ccgatgtcct gcaggcgcac ctgctggacg agctgcccga
     2761 tctcgcggcc gacctgcatc ggcgggcgac cgagtggttc gagcagaacg gcgaacgctc
     2821 cgaggcgatt cgccacgcga tggcgggcaa ggacttcgcg cgagcggcgg acctggtcga
     2881 gctcgcgatg ccggcgacgg gccgggaccg gcaggaggcg acgctccgtc ggtggctgga
     2941 ggcgctcccc gacgagctga tccgggccag gcccgtcctg agcaacgggt acgccgggtc
     3001 gatcctcgtc aggggcgaga ccgacggcgt cgaagcgcgt ctgcgggacg ccgagggctg
     3061 gctggacggc gcggccgagg ccccggacga ggtgggcgtt cgccctgctt cgatggtcgt
     3121 cgcggacgag gatgcgttcc ggaggcttcc gggcatgatc gccatccacc gggccggaca
     3181 ggcccgactc ctcggcgacg tcgccggcac catcgcccat gcccgccggg ctctcgacct
     3241 cttcgacgac gacgaccagc ttggccaggg aggggcgtcg gcactcctcg gcctcgccta
     3301 ctggacgagc gcggatctcg aggcggcgtc cggcttgtat gccggtgcca tgccccgatt
     3361 cgagaaggcc gggcatctct ccgacgcgat cgggctcgcc ctcgccctgg ccgacatgcg
     3421 gatcgcgcaa ggccgtctcc gcgaggcgat gagcacctac gagcggggac tcgagcttgc
     3481 gaccggtcaa ggcgcgctcg cgctgcgagg agcagcggac atgcacgtgg gcatcagcga
     3541 gatcctccgc gaacgggatg acctgatggg tgccgcgcag catctccacc aggccaggga
     3601 ccttggcgac gagaacggcc tgccgcagaa cccctaccgc tcgcgggttg cggccgcccg
     3661 gatccggcag gccgaagggg atctcgacgg gtcgctccag ctccttgatg aggccgagcg
     3721 cctgtatgcc aacgacttct ctcccgatgt gcgcccggtc gctgccctga aggcgcgcgc
     3781 gcgaatcgcg caggggaagc tgtcggaggc gtcggcctgg gcaggtgacc ggggcctgtc
     3841 gtccgccgac gacctgagct acgtccgcga gttcgagtac gtcaccctgg cccggctgct
     3901 ccttgcgcag ggatcgcgcg accgctccga ccagctcgtg gctgaggcga ccgatctcct
     3961 ggagcgtctg ctggtggccg cggagagcgg cgggaggacg ggaaccgtca tcgagatcct
     4021 ggtggtgctg gcgctcgccc ggcatgcccg cgacgacgtg accggtgcgc ttgcgtcgct
     4081 ggggcgcgcg gtggcgctgg ctgagcctga gggctacgtc cgcatcgtcg tcgacgaagg
     4141 gcggccgatc gcggcgttgc tgaagctcgc ggcgaagcag ccgaccactc cgacctatgt
     4201 gcgccggctg ctggccgctg ccgtgccggc ggatggacga acgcccatcg aacagtcgct
     4261 gatcgagccg ctgagcgaac gggagctcga ggtcttgcgt ctcctggaca gcgacctcga
     4321 cggcccggac gtggcgcgcg agctcacgct gtccctggcc acggtgcgga cgcataccag
     4381 gaacatctac gccaagctcg gcgtgaacaa ccggagggca gccgtccgcc gtgccgacga
     4441 gctcggcctg ctctcgcgca cccgagaccg ccgaccaagc gtgtagtcct gcgcgacgcc
     4501 gccccgaccg cggccgcaga acccgccggc gattccatca gctcgctcat cacgtgtggt
     4561 gatgcgcgct catcacatcc gcctccacgg tgttcccagt gagtcaggtc ggtgttgccg
     4621 gccggagctc cagtggcaac acgtacgagg agcggaatga ccgatcaacg gaccccgaca
     4681 cccgatcgcc ccgaggccgg gcgctatgag atccggctca cggggcacct cgacgcgcat
     4741 tggaccacct ggttcgacgg attgagcgtg agccgtgagg tcgacggaac caccgtcatc
     4801 agcggcccga tcgccgatca atcggcactc cacggcgtgc tccaacgagt gcgcgacctc
     4861 ggcctgccgc tggtctcggt cattcgcatg aagcccgacc agcccgaaac gaccggcatc
     4921 aaccccggct agaacccgat cacgaaggag atcagcacga tgatcacgac acgcatccag
     4981 acgatgaccc gcctgttcat ggccaccgtc tcgctcgccg cggtggcagt tgctctcgtt
     5041 gctgctccgg ccgcgggcgc agcaggcctc cggaactgcg tcgacgtgac cggaccgcag
     5101 tccggccgcg ccggctgcta cgaggacgtc tgggcgaacg gtgcccagta ccgcatgacg
     5161 ttctcgaaca cccgcttcac cggcgcgacc ccgaaggcac ttgacccctt ctacgtgatc
     5221 gcaccgcaga cggacaggcc gcagggcgcg ccgcccaaca cgttcgcgca cgaccacgtc
     5281 gtccgcaacg tgccgcacca gaaccacggt gagtacagcg tcaagatgca ggggttcttc
     5341 gtcctgtgca gcggccaggg catcgtcagc ggcgcctgcg tgccgtcctg gacgtccatc
     5401 ggtggtcccg atccacttcc gttcgcgaag accgtcgacg gacagccact gacgtccacc
     5461 gacgcaatcg aggccgccgc ggccacaggc aacctcgcgc tgatcaacct cggccccggg
     5521 gccgtgatca tcggggccgt caccggcagc aactgaccga tccgtgcacc aggtgcgggc
     5581 gacctgatgg ttgcccgcgc aatcccacac cactgaatcc ccgaaaggaa cccgacatga
     5641 caaacatcca gcggaccacg acggcgaagc gaaccccgat ggacgcgtct cgaaaggtcg
     5701 cgctcgccgt aggcgtcctg ttcatcctca ccttcgtcac ctcgattgcc ggggtcttcg
     5761 cctacgggcc cgtcctgagc gatccttcct acatcaccgg cgccggcccc gacacgcgcg
     5821 tgttcgtggg cgcgttcctc gagctcatgc tcatcctgac caacatcggc tgcgccaccg
     5881 tgctgttccc gctcctcaag cggcagaacg aaggcctcgc gctgggctac gtcgccgccc
     5941 ggatcgtcga gtgcaccttc atcctcgtcg gcatcctcag cgtgcttgcc atcgtgacgc
     6001 tgcgccagac ggcgacggcc gcggatgcgg gctcgatcct caccatcggc aaggcgctcc
     6061 acgcgatcca cgattggacc ttcctgttcg gccccggctt cacggacggc atcgggaccg
     6121 gactgatcct cggctggctg atgtaccggt cgggcctggt gtcgcgacgc atggcgctct
     6181 tcggcgtcgt gggtggtccg ctcctcgccg cttccgggat cgccgtgctc ctcggtgtca
     6241 tcccgcaggg ctccgcggcg cagggcatcg cgacgatccc ggagatcgtc tgggagatgt
     6301 tcctgggcct gtggctcacg ttcaaggggt tcaaggccac cgctccggcc gccgttgcct
     6361 tcgactcgga cgctgccgag cagtcggcga cggccgccgc cgggccgatc ggtgtcgcga
     6421 cgaaggcggg cgcggcatga ccgccgtgcg cgagcaggcg ccgcggacca tgccgcggtt
     6481 cgtccttcgc atcttctgga tgctccaccg cggcatcgac cggctcaccg ggggcagatt
     6541 cggcctctcg cggccggagg ccggcaggaa gttcgggatg cttcggctcg ccacggtggg
     6601 tcggcgctcc ggccagccgc ggatcgcgat cgtcggctac tacgaggacg gcccgaacct
     6661 cgtcacgctc gcgatgaacg gatggggtcg ttcggagccc gcatggtggc tcaacctcca
     6721 ggcccggccc gacaccactg tcgggctggc cgatgggccg cgggccgtcc gggcccgcgc
     6781 ggcgaccggc gccgagcgtg agctcctgtg ggcccggttc gccgacttcc ccggctgggg
     6841 tgccgacatc gacgccctcg ctgcgcggcg cccggacgag accaccgtgg tcgtgttcga
     6901 gccgcgcgac gacgcgagga tcgtggcatg acggccacca tcgtcgctcg gccccggcga
     6961 cctgcaatcc ggcatggctg gctcgtgccg gggctggccc tcgcggtcta cgccaacaca
     7021 ctggccgcgg accacggcct gggcctcgtc ccggtcctgc tgttcgggat cgctccccac
     7081 ctgacggtcc tggtggggtt cggccagccg catgcgcggg ggcagctggc tccccgcgcc
     7141 gtaccgctct tcgacacgat gcatcaccct gctgtaccgc tcgcggtgct gggactcgcc
     7201 gcggcgggcg tcctgccgcc gttctggttc gtcgccgcgc tcgcgtggct cgggcacatc
     7261 gcggtcgacc ggggcttggg ggacgggctg cgaaccgcgg atggatggca gcgtcgctga
     7321 gggacgccgc gcctggatct cgcgactgcc tgcgcgggct ggccaacgag aaccgtgttc
     7381 gacacggctc gccgtgagcc ggggcgtcgc gatcccgggc cgcggccatt ccgaatagat
     7441 caggccggcg tcgacggggt cactgaaacc ttcagttgcg gcctgtagga taggccgggc
     7501 tcgcgccgtg cgatccaccg aaccgcggac gcgtcgtgcg cttcgcgacg acgctccggc
     7561 ggcgacatga cctggagacg cgacgacatg gctgagacgg agagcggcaa acgcgtgacg
     7621 ctcgccgacg tcgccgccgc ggccggcgtc tcgcccgcgg ccgtgtcgct cgccgtccgc
     7681 ggcgaaccgg gcgtgagcga ggagacgcgc cggcggatcg tcgagacggc tcgcaggctg
     7741 ggctaccgga cggtcaaccg ctggggcagc cggcgctcgc gccagacgac gatcggcctg
     7801 gtcatcaagg cgccccaggg cgatgcgccc aaggcgaacc gcttctacgc cccggtcatg
     7861 gccgggatcg aggagagttg tcgtctgcgc agcgtcgacc tcctcctcgc gacgatgccg
     7921 gtggacgcgg actaccaccc gatcgaggtg ccccggctcg tcgcggagcg agccacggac
     7981 ggcctcatcg tcgtcggcgc gcacctcagc cggggcacga ccgaactgct cgaggacggc
     8041 ccgcccgtcc tcctcgtcga tgcctatgcg gaagatgccg cgttcgattc cgtggtcagc
     8101 gacgacgtcg gcggcgcccg agcggcggtc gagcacctga tcgccaaggg ccaccgcgcc
     8161 atcgccatcg tcggcagcca tccgggctcg ttcccgagca tcctccagcg ccgtcgtggg
     8221 tacgagcagg cgatgaccga ggccgggctc cagccccatt tcgccgacgg accgcacgtc
     8281 ccacccgagg tcggcgccgc ggccggcctc gactacgtct gtgcgcatcc ggagatcacg
     8341 gcggtcttct gctgcaacga cgacgtcgcc gtcgccttca gccagtcggc ccagcgaacc
     8401 ggaatcgtcg tgcccgacgc gatgtccgtc gtcggttacg acgatatcga cctggcggcg
     8461 ttcgtcacgc cgcgcctcac cacgatggcc gtcgacaaga tcgggatggg ccacctggcg
     8521 gtgaccctgc tgctccaccg gctggagctc gggagcgcgg cggtgaccca gaccctgatc
     8581 cggccgcgcc tcgtcgagcg tgaatccgtc gcgacccggc tgccgagctc ctagcggatc
     8641 gaggcgagga tctccgagag gtagccgtgg gcgatgcaga ccaccgtgtc ggccgcgccg
     8701 tagtagatcg tgatccgctc gccgtccgag tcgagcacgg cggcgcaggg gaagacgacg
     8761 ttcgggacgt cgcccacctg ctcgtacgac acctgcggcg acaggaggta gtcgcggccg
     8821 cgcgcgatga cctgccacgg ccgctcgagg tcgaggagcg cggctcccat cgagtagacg
     8881 aagccgttgg ccgacgtcag cacgccgtgg tagatcagca gccaaccttc gctggtctcg
     8941 atgggcgtcg ggccggcgcc gatcttggtc gactgccagg tccacggctc cggggccagg
     9001 acatggcgat ggcggcccca gtgcaccaga tcggggctct cggacacgaa gatgtcgccg
     9061 aacggcgtgt gcccgttgtc gctcggccga ctcagcatcg cgtacttggc gccgacccgg
     9121 cgagggaaga ggacgccgtt acggttgaac ggcaggaacg cgttgtcgag ctgatggaag
     9181 gtctcgaagt cgtgggtgta gccgatcccg atcgtcgggc cgtggtagcc gctgcaccac
     9241 gtgacgtagt agcggtcctc cagccaggtg acacgcgggt cgtaggcatg ctcgaaggcc
     9301 gcctggatct cggcgacgcg gccgtcggcg gcgtggaacc ggatcggctc gggctcgatg
     9361 tcccagtcga ttccgtcgcg gctgcgtccg gcatgcaggt tcatctcgcg tgaacgatcg
     9421 tcgactcgga agatgccggc gaagccgccg ccgtggggga cgaccgccga gttgaagatg
     9481 ctgttcgagc ggggcaggtg gtcgcgccgg atgatcgggt tgcggctgga ccgccacagg
     9541 acgccgttct ctgcggccgg ccgatcctcc cacgggatcg gtgccctggc ggcggccccg
     9601 gggagggacg ttcgcggagc gagctggggg gtcaggacgc cgtccgtcgc tgcggcgtcg
     9661 tccatgtcga gcggggccgg cctgtcgtcg gccgtgacgg cggttccagg cgtggacggc
     9721 tgggactcgg gcatgtcgtc tcccattcct ggcgggatcc ggccgcagta tagcaggcga
     9781 acactcaaga agactcaaga gttgagtcaa tattctactg ataccacttg acaccaacat
     9841 gccgccggac gtagcgtggg ccgcgcttcc cggaggagat cggtagccat gcccgttccg
     9901 gcatgtgcct tggagcctgt cagtcgagct gaccgaggag gagcccgcga cccgacctgc
     9961 cgtgactcat ggggcggtac ggcgagcgat atcgcctacc cgtcacgtgc ggccatgcac
    10021 cgccgcatgc ccccctcgac cccgccattg ggagatcggt atgggaaagc gaatctcggc
    10081 gatcctggca acgttgttgt tcgtgtccgt ggcggcctgc tcgggcagcg ccacacccag
    10141 ccccagcgcc agctccgtcg cctcggcccc cgtgaccggc tcggcccagc ccgcgtcggt
    10201 ggcgccatcg gtggcgacgg tgtccggtga gatcaccgtc ctcacgaacc gcaccgacat
    10261 cgtcgacagc gtgttcacgg ccaagtacct gccgctcttc aacgccaagt atccgggcgt
    10321 caaggtcaag ttcgaggccc tcaccgacta cgagggcgag acgaagaccc ggatgaacac
    10381 gaccgagtac ggcgatgtgc tcctgatccc gaacgcgatc acgccggaca agctcgccga
    10441 gttcttcgag ccgctcggga cccttgacga gctcagccag aagtacaact tcacgaccga
    10501 gcagttgtcc aatggcaagg tctacggtct cgcggtgacc ggcaacgcca atggtttcgt
    10561 ctacaacaag aaggtctggg atgcggccgg ggtgacgtcg ctgcccaaga cgccggacga
    10621 gttcctcgcc gatctcaagc tcatcaagga caaggagtcc agcgtcacgc cgtactacac
    10681 gaactacaag gacggctggc ccctgaccca gtggcaatcg aaccggggcc agatcaccaa
    10741 cgacccgaac tacgccaacg gaaccctcgt ccacgccgac gcgccctggg ctgcgggcac
    10801 cgacgagaac gtcatctaca gcctgctcta caacatcgtg aagcagggcc tcagcgagaa
    10861 ggatccgacg accacggcgt gggagccgtc gaaggctctc ctcggtggcg gcaaggtcgc
    10921 gacgatgcag ctcgggtcgt gggcgatcgt ccagatgcag gaagccgccg tgaaggccgg
    10981 cgggtcggcc gatgacatcc actacctgcc gttcccgaac caggtcggcg gcaagttcat
    11041 ctcgaacatc ggcggcgact acaagatcgg gatcaacgtc cattcccaga acaaggaagc
    11101 cgcccgggcg tggatcgact ggttcgagaa cgagtcgaac ttcgctttcg accagggcgg
    11161 tgtctcgccg gtgaagggtg gcaagaatcc gccccagctc agtgatttcg acggcgtcgg
    11221 cgtcgcctac atcgagcagg ctcccgccgc ggccggcgag gagggtctgc tcggcaagat
    11281 ccaggaccag tcggagatcc agctcttcga ccaggcatgg cctcagcgca tcgtcgacgc
    11341 agctcgaggt gcgaccaacg aaggccttga tgcgatcttc gccgacctca acgggaagtg
    11401 gaaggctgcc cgggcgaccc tctcgatcaa atagcgcgga aaccgaccat cacgcctgat
    11461 atcgacccgg gtcgatcgga ccgcccagcg gcgatccgat cgacccgcca ccacgcccgc
    11521 caccgcatca tgcggacatc cgtattcgcc gcggagcacg acatgacgat cggccgaacc
    11581 cggtgacgac cgtcaacctg acgaaggcca ccgcccggac ctcgcgggcc gcgactcccg
    11641 gcccaggtca ccgccgcccg tggacgccgt tcctgttcct ggtcgtgccc gtcggactgc
    11701 tcctgctgct gacctacctc ccgctcttgt tcatggtcca gttcagcgtc ttccgctggg
    11761 acgggttcag cagcgaccgg accttcgtcg ggccgcagaa ctacatcgac atcttcaccc
    11821 ggccggagct gttcggcgtc ttcttcgtca gcctgtacta cttcgccgcc tcattcgtcc
    11881 agatcggcgt cgccctctat ctcgcgaccc ttctgagctt caaggtccgg ttcaagaacg
    11941 tcttcaaggg gatcatcttc ttcccgtacc tcatcaacgg cgtcgcgatc gccctcatct
    12001 tcacgtactt ctttcgaccc gacggcgtgc tcgattctct tctcgcgatt ggcggggtga
    12061 acgatccgcc gctgtggctc ggggatcggc gcctcgtcaa tatctcgctc gccggtgtct
    12121 cggtctggcg ctacatgggg ctgaatttcg tcctgttcct cggtgccatc cagtcgatcc
    12181 cgtccgagat gctcgaggcc ggcgagatcg atggggcaag ccgctggcag cggttccggt
    12241 acctcatcgc cccggggatc cggccggtca tcgccctgtc gttcatcctg gccatctcgg
    12301 gatccctggc ggtgttcgag atccccttca tcatgctgtt cggggccaac ggcagcgcga
    12361 cgttcgtgat ccagacggtc cagacggcgt tcacattcca caagttcggg ctcgccgcgg
    12421 cgatggccgc ggtgctcctg ggactcgtgc tcctcgtcac gttggtccag agacggcttc
    12481 tccccgacga tcccgtggag ctcaccgtat gaccgagccg tcgacgcgtc tccgggtctg
    12541 gctgggccgg acgatcacct acggctctct cgggctggcc tgtctcgtgg cgttgctgcc
    12601 cctcgtcgtc ctgatgatgg cctcgctcaa gacgagcaac gagttcgcga cgcagggcgt
    12661 cctcgatcca ccgggagact ggttcaactt cagcaatttc gcggcgttct tcgtcgaagg
    12721 caacgttgtt cagggcttcc tgaacacgag cttcatcctc gtgatctcgg tgatcgggac
    12781 ggtgatcatc ggctcgatga ccgccttcgc gctcgatcgg ttcgagttcc ggctcaagcg
    12841 gaccgtggtc ctgctcttcc tgttcgcggc gctcgttccc accgtgaccg cccaggtgac
    12901 cacgttccag ctcgtggcca acatgcacct ctacaacacg cgcttcgccc cgatcctctt
    12961 gttcatcggg accgacatcg tttcgatcta catcttcatg cagttcatgc ggggcatccc
    13021 gcggtcgctc gatgaggtcg cgttgctcga tggcgcgtcg caattcacga tctatcgcaa
    13081 gatcgtcctt ccgctgatga agccggcgat cgcgaccgtc gtgatcatca agggcgtcgc
    13141 gatctacaac gagttctaca tcccgtacct gtacatgccg tcgcgggacc ttggcgtcgt
    13201 ggccaccacg ctctcgcggt tcgccggccc acagtcgacc cggtgggagg tcatcgcggc
    13261 cggggtcgtc atcacgatga tcccgaccct tgtcgccttc ctcgtcctgc agcgctacat
    13321 ctacaacggc ttcacccgcg gcgcggccaa gtagttcgcg gcgacggccg ccgcggccgc
    13381 gggccgacgt tcggtcaggc ccgcaggagc tccccgagcc agcgcaacct ggccacgtcg
    13441 tggaagccct ggccgccttc gtggtcgttg aacgggtact cgtggatctc cttcggaccg
    13501 ccgtaggcgt tgtaggcggc gtagaccgtg gacgggggac tgatctcgtc catcaggccg
    13561 accgagaaca gcgccggcgc cgtggctcgt cgcccgagga tcgcggcgtc gaagtaggag
    13621 agcgtcgcca gggccagctc gacgtggtcg cgatgagcct tgaggtagcg gacgatctct
    13681 ccgtacgggt cgctgtcggc gaaggtgatc gcgcgcggga agtcgcacag gaacggaacg
    13741 tcgggcatcg ccgcggccac gtcgggcacg agggcggcga cggccaggct gatcccaccg
    13801 ccctggctcc caccggtcac ggcaacccgc ccgggatcga ccgcgggatg catccggatc
    13861 gcgtcgatcg cccggacggc gtcggtgaac acccgccgga agtagtaggt ccgcggatcg
    13921 aggatgccct gggtcatgaa ccccggatgg aacggggcac cgcccgcatc ggggtccgcg
    13981 gtctcgccga cggcccacgc cgatccctgg ccgcgcgtat ccatgacgag gtgggcgtag
    14041 cccgccgcgg cccacaggat ccgttcgtgg gcgagcccgc gaccgccgcc gtagccgacg
    14101 tactcgacga cccccggcag gggagcatca tgaccgaccg gcaggtgcag ccaggcgcgg
    14161 accggcgacc cgccgaagcc cgcgtacgtg acgtccaggg tcgagatcac cgccaggtgg
    14221 ttgtccatcg gctcgaatgt cgcgcccagg tcgtgacctc gcgcatcggc cagcgtggtc
    14281 gaccagaacg cctcgaggtc ggacgggacg gcgagatcgg gcgcgtacga gcgcagcacg
    14341 gcgatgggca ggtcggactg aggcatcggg gttggttact cctggctgat gcgcgagctc
    14401 agcatcagct cgagcaggtc gacatcgaca gggtaaccgt ggcgcatctc gatccgcgcc
    14461 ccgggacggc cgtggccgac gatcccgaac gggccctcca ggttccacat cgcccagccc
    14521 cagccgaact cgcggaacag gccgaacagg tcgcggaacc agcgcatggc atcgtcgttc
    14581 ggagtccggt tgtagcaacc gagctcaccg atgtgaacgc cgaccccggt tcgctcgacg
    14641 gcccgccacg gctcgtagaa tgctcgcagc gcggcgggcc cccaggtggt tccctcgaac
    14701 tcgacgcccg ggtagcgggg cgcatcgcca cccttccagc cgtcccacca ctcggctccc
    14761 caatgcgaga ccggataggg ctggtagccc cggccaccgt ggatgatccc gaggtcggcc
    14821 agctccggca tcgcgaggtt gccgccgtcg agcccgtcga tgacgatcgg ccggtccgga
    14881 tcgatggccc ggatcgcagc gacagtccga cggatcaggg cggcatgccg gtcacgggtg
    14941 aagccatcca ggccgatcgc cggcggctca ttgaggagat cgaagctcag tgcctcggcg
    15001 gcggtgccga ggtagcgccg ggcaaagccc tcccacaggc cgacgaaccc atcctgcgcc
    15061 acgcggtcga gccagaggtt gtcccgctcc aggtcgttcc gggtgatgca gtaaccgggc
    15121 gcgcgatgga ggttgagcga caggtggatc ccgcgcaacc gacacgccac gaggtggtcg
    15181 tcgagggtgg cgaacaccga ctcgtcgggg tggccgtagt cgaagccggt cgtccaggtc
    15241 cggtagtcgg tcggcatccg gacgaagttg aagtcgagcc cggacaggaa gtccagggcc
    15301 cgctcgtcgg gcggtagcgg tcgacgcccg ccgccgacca gcatccattg catgttgaag
    15361 ccataccagg ccatccgcga tcgacctccg tggcgcgggg cgagatgctc cgggccttcg
    15421 ggctgggcat ccgtgtggcg tcgagcccgg tcactaacgc atcaagtgac tattcactat
    15481 cggttacttg acgaaccggg agagcgtacc taccgttcag ccgtggacgc aagcccgcga
    15541 ggcgtgcgcc gacaccggtt gccttccgga tcgccccggt tcggcgccgg tctgcggctc
    15601 cggggcggcg ggccgccatc tcctccggcg gctccccccc ggcccgccgg tcgccggcgc
    15661 ccagagtgat ggctgatcgc tccgtcgcgg ccggtccatc gggcggtgct gcaggcccgc
    15721 tccgatcgcg acgaaggcgg ccctcagcgt ccccggtccg tctcggtcac gcgaggtccg
    15781 cgccgacagt ccggctcagg cctcgctgaa catcgtccgc ggtgacgaaa cgaccctcgt
    15841 ccggtgggac gagggtcgtc gatcagcttc gagggtgcgg gccggcgcag gccggcaatc
    15901 gggtcgtcag tcgacccgct cgaggaggat catcgggatg acccggtcgg tcttcgccgg
    15961 gtactcaccg aacgccggca gcgcggcgac gtgatcgttc cagaggcgct cgcgttcctc
    16021 gccgtccgcg atcagcgccc gcaccggcac cgtctcgttg gcgacctcga tcgtggccgt
    16081 cgggtgagcc tcgaggttgt ggaaccatgc cgggtgggtc ggcgcgcctc ccttcgagcc
    16141 ggcgatcgcc cagcgatcac cgtccgcgtg gtaggtcacg atcgcctggc gcggctcgcc
    16201 ggacttcgcc ccggtcgtcg tcaggatcat cagcggctgg ccggccatgg gtcccgccga
    16261 cgcccggccg ccgtgggctc gcatgtcggc gatcaggttg cgggtgaagg cttcgggatc
    16321 ccagtcagtc atgcgagtgt catctccctg tcgtgagcgt cgtcattccg ttgcatgcgc
    16381 aacgattgca cggatcgccg gatcgcgccg acgcgggctc aggtccgcag ctcggccggg
    16441 gtcaacgcga gctcggccag gatcccgtcg cgctcgaggg tgacctcgat cgaccgaccg
    16501 accaggtcgc cgaccagcga gcgctggaga tcgccgaccc ccgcgatcgg gcggccgtcg
    16561 agcgacacga tgaggtcgcc tgcccggacg ccggcggcgg cggccgggct tccgtcgagc
    16621 agctggacga cctcgaggcc tgccgcgcgt ccgagccgat cggccagggc ggccggcagc
    16681 cgccgcgttc cgccgacgat cccgagatag gcccgccgga cgcggccgtc gcgcatgagc
    16741 gcggccagga tcaggcgggt cgcggcgtcg atcggcacgg ccaggccgag gcccacgccg
    16801 gcgacggccg tgttgatccc gaccacgcga ccccggacgt cggccaacgc gccgccggag
    16861 ttgcccgggt tcagggcggc atcggtctgg atgacgttct cgacgaggcg gctggcctgg
    16921 ccgtcgcgag ccgccaggga gcggccgagg gcgctgacga cgcccgcggt caccgacccg
    16981 gcgaacccca gtggattgcc gatcgcgacc acgagctgcc cgacccggag ccgcgcggca
    17041 tcaccgaacg gcgcggccgc gagatccgca ccggaggatc gaacgaccgc gaggtcggag
    17101 agcggatcgc ggccgacgac ctcgaacgag agctcgtcgc cgtcgacgaa tgtggcggtt
    17161 ccccgttccg cgccggcgac gacgtgggcg ttggtgacga ggaacccgtc cgcagtgaac
    17221 gcgacggccg aaccgctgcc gccgccccag ccgccattcc ggcggtcgac ccgcaggctc
    17281 gcgaccgacg ggatgaggcg cgccgccgcg gtgctgaccg ccgagctgta ggcgtcgagc
    17341 gcctcgtcct cgatcgccgc ggtcagggcg tcgtcggccg cggcggcggc cgcggcgtcg
    17401 tccggctcga gttcacgcag ggcgaccatg ccgatcaggc ctcgcccgac gcgtcggtcg
    17461 tgccgccgac gctgaccgtc cgctcctcgt cgccgcgaac gatcttcaga gcaatcggca
    17521 ttcctgcctc ggcgaggatc gccggcaggc tgtcgacgtc cgtcagcggc ttgccggccg
    17581 cctcgacgag caggtcgccc tcgcggatcc cggcgcgatc ggcggggctg ccttcctcga
    17641 cgccgcgcac gagcaggccg tcccgttcgg caagaccgac cgagcgccgc agccgctggg
    17701 cgacgaccga aggagccacc gcgatgccga gccgcggtcg gctgaccgac tcgccccgcg
    17761 acagggcgtc gacccgcacg cggaatgcgg cgtcggcggg acgggccaga tagaagccct
    17821 cgccgatgcg gttggtgttg agcgcgacga ggcggccgct gccgtcgacg agcgggccac
    17881 cggacgaacc cgatgcgaga ggcgcggtgt gctcgaggct gccgccgatg cggctcccac
    17941 ccgggccgcg gaacgtctgc tcgatcgcgg agatcgtgcc ggccgtgacg cgcgccgcgc
    18001 cggcgccggt cgacgcgaga ccgaacacga catctccgat cgtggtttcg acatcggacc
    18061 aggcgagggt cgtcgccccg gtcgtgtcga cctcgatgac cgcgagatca ccgtcgggat
    18121 cgacgccgcg gacggtgccg cgctcggacc gtccgttgcg gaaggtgacg gtgacttcgt
    18181 cgccgcgaag gttgtgggcg ttggtcagga cccggccgtc gccgatgacg acgccgctgc
    18241 cgcggtttcg ctggccgatc ccgacaacgg ccgggccggc cgactcgtgg acgctgcgga
    18301 tcgcactgga aagatcgtcg aggatggaca tcggggtcgc ttctccggtt cgcgggtggt
    18361 ggggccgagc gccggacgtg gccggcggcg ttcttgccgt ccggtccagt ccgtagtcgg
    18421 ttgcaattcg caacatacaa cggcgtcgtc gccggtgcaa gtcccacggt aggatggccg
    18481 gcgtgaccga gcccgccacg gcccctcctt ccccgacggc cctcgaatcc gccctcgatc
    18541 gggtcggcga ccgttggtcc ctgctcatcg tcgacgccct cctcgacggt ccgcggcgct
    18601 tcggggagct gcgagacgcc ctgccgggca tcgccccgaa catcctcacc gatcggctcc
    18661 gccgcctcga gcgggagcgg attctcggat cgacgcccta cagcacgcgg ccgccacgga
    18721 tggaataccg cctcaccggg gatggccgcg atctggcgtc cgccctgcgc ctcctcgccg
    18781 actggggcag ccggcgaacg ggcggtgagc cgctccgcca cgaccgctgt ggaacggcga
    18841 tcgaggcgcg ctggttctgc ccgacctgcg ccgtcgcggt gggcgacgcg gagatcagcg
    18901 gggccgagga gacgcgcctg gtctgagccc cggacctggc ggggcctacg tcggccggcc
    18961 ggtcaggccg gagcgtcctg ggcgcctacc ccgtccgatg agcaggacgc cggtcacccg
    19021 cgagtagtgc gagtagtacg tcgtgcggtg gaggaccttg ccgtcctggt acgtcacggt
    19081 ccgcccgacg tcgatcccca actccgggcc gtttcgccgg gcgtgtgggg gtgaggttcg
    19141 cggccctgct gccactcggc gggatcaagg ccgtggtgac gggctgctgc cacgcactcg
    19201 ccgccgccaa gatgtctgct cctcgccaga ctaggc
//
LOCUS       DATAIW010000436         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_222808, whole genome shotgun sequence.
ACCESSION   DATAIW010000436 DATAIW010000000
VERSION     DATAIW010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222808"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..652
                     /locus_tag="VHM48_13115"
     CDS             143..652
                     /locus_tag="VHM48_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2756403.1"
                     /translation="MNELTESAVAARVPFLIDERLQVLARRAPVTVAPNTSLADCLAA
                     IHRSGTGDSVFVVDADGRLAGVLTERDIFSRLVGGDVAPIDLDQPVETLMTTKPHHLH
                     LDETVRDAIELMQTGRYRNVPLLDDDDVLQGVIRPQDILKYLAEAFPEELLNLPPRPH
                     QTMERAEGG"
     gene            660..>2476
                     /locus_tag="VHM48_13120"
     CDS             660..>2476
                     /locus_tag="VHM48_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2756404.1"
                     /translation="MTTIRETEPQTQELDRVTIRFAGDSGDGMQLTGTQFTRNAAVYG
                     NDISTLPDYPAEIRAPAGSLPGVSGFQISFSSSEIHTPGDQPDVLVAMNPAALKTNLV
                     DLPAGGALIVNSDAFTAANLTKAGYAANPLQDGSLKQYALFEIPISTLNELSLDGLDL
                     TSKQKDLTKNFFALGIMFWLYGRDYDGTVRWIDEKFGKRPIIAEANTRAVKAGYAFGE
                     TTEIFHTHYRVAPAHLAPGTYRNITGNEATALGMLAASKLAKRPLFYGSYPITPASDI
                     LHQLSGYKNFGVKTFQAEDEIAAIGASIGASYGGAMGLTASSGPGIALKSEAMGLAVM
                     VELPLVVVDVQRAGPSTGMPTKNEQSDLLQVMFGRNGDSPLPVVAPATPGDCFDMAIE
                     AWRIALKYVTPVVYLSDAFLANGAEPWKIPDIATLPDISVANWTEREGFHPYERDPNT
                     LARKWAVPGTPGLEHRIGGLEKADITGNVSYDPDNHHRMQLLRQAKVARMADDIAPLE
                     VFGPERGDLLILGWGSTYGAIRSAVERMHGQGRGNVAHAHLRHLNPFPANTEKVLRSY
                     RRVLIPEVNLGQLLMLIRSQFLIDAEGYDRVRGKPFTIA"
BASE COUNT          427 a          903 c          780 g          366 t
ORIGIN      
        1 ctggcgggga gcatgcatcc tgggcggttg cgccggatcg ggcgacggcc gtccgttcct
       61 tccagacatc tcgcagtgac agcgcgccgg gggccgaccc gggttgagcg gttcccgctc
      121 cgcgaacggc tacacttgga gcgtgaatga gttgaccgag agcgccgttg cggcacgcgt
      181 cccattcctc atcgacgagc gcctccaggt cctcgctcgc cgcgcgccgg tgaccgtcgc
      241 cccgaacacc agccttgccg actgcctcgc ggcaatccat cgcagcggaa ccggcgattc
      301 agtgttcgtc gtcgatgccg acggacgcct ggccggggtc ctcaccgaac gcgacatctt
      361 cagccgcctc gtgggtggag atgtggctcc gatcgacctc gatcagccgg tcgagacgct
      421 gatgacgacg aagccgcacc acctccacct tgatgagacc gttcgcgatg cgatcgaact
      481 gatgcagacc ggccgctatc ggaacgtgcc gctgctcgac gacgacgacg tcctccaggg
      541 cgtgatccgg ccgcaggaca tcctcaaata cctcgccgag gccttcccgg aggagctcct
      601 caacctgcct ccgcgtccgc accagaccat ggaacgagcc gaggggggct gacgaccacg
      661 tgacgaccat ccgcgagacc gagccgcaga cgcaggagct cgatcgcgtc acgatccgct
      721 tcgccggcga ctccggcgac ggcatgcagc tcaccggcac ccagttcacc cggaacgccg
      781 cggtgtacgg caacgacatc agcacgctgc ccgactaccc cgccgagatc cgggcccccg
      841 ccggctcgct gccgggcgtg tcggggttcc agatcagctt cagctcgagc gagatccaca
      901 cgcccggcga ccagcccgac gtcctcgtgg cgatgaaccc ggcggcgctc aagaccaacc
      961 tcgtcgacct gccggccggc ggagccctga tcgtcaacag cgatgcgttc actgcggcca
     1021 acctgacgaa ggcaggctac gcggcgaacc cgctccagga cggctccctc aagcagtacg
     1081 ccctgttcga gatcccgatc tcgacgctca acgagctctc gctcgacggc ctcgacctga
     1141 cgtcgaagca gaaggacctg acgaagaact tcttcgccct cgggatcatg ttctggctct
     1201 atggccggga ctatgacggc acggtccgct ggatcgacga gaagttcggg aagcgcccga
     1261 tcatcgccga ggcgaacacg cgggccgtca aggccggcta cgccttcggc gagacgacgg
     1321 agatcttcca cacccactat cgcgtcgccc cggcccacct cgcccccggt acgtatcgga
     1381 acatcaccgg caacgaggcg acggcactcg ggatgctcgc ggcctcgaag ctggcgaagc
     1441 ggccgctgtt ctacggctcg tacccgatca cgccggcctc ggacatcctc caccagcttt
     1501 cgggctacaa gaacttcggg gtcaagacat tccaggccga agacgagatc gcggcgatcg
     1561 gcgcctcgat cggcgcgtcg tacggcgggg cgatgggcct gaccgcatcg tccgggccgg
     1621 gcatcgccct gaagtccgag gccatgggtc tcgcggtcat ggtcgagctg cccctcgtcg
     1681 tcgtcgacgt ccagcgggcc ggcccctcga ccggcatgcc gaccaagaac gagcagtcgg
     1741 acctgctcca ggtgatgttc ggtcggaacg gcgactcgcc gctgccggtc gtcgcgccgg
     1801 cgacgccggg ggattgcttc gacatggcga tcgaggcctg gcgcatcgcc ctcaagtacg
     1861 tcaccccggt cgtctacctg tccgacgcgt tcctggccaa cggcgccgag ccgtggaaga
     1921 tcccggacat cgcgaccctg ccggacatct cggtcgcgaa ctggaccgag cgcgagggct
     1981 tccacccgta cgaacgcgac ccgaacaccc ttgcccggaa atgggccgtg ccgggcacgc
     2041 ccggtctgga gcatcggatc ggcggcctgg agaaggccga catcaccggc aacgtcagct
     2101 acgacccgga caaccatcac cggatgcagc ttctccgcca ggcaaaggtc gcccggatgg
     2161 cggatgacat cgcgcccctc gaggtcttcg ggccggagcg cggcgatctc ctgatcctcg
     2221 gctggggctc gacctacggc gcgatccgga gcgccgtcga gcggatgcac ggccagggcc
     2281 ggggcaacgt cgcccatgcc caccttcgcc atctcaaccc gttcccggcc aacaccgaga
     2341 aggtcctccg cagctatcgc cgggtcctga tcccggaggt caacctcggc cagctgctca
     2401 tgctgatccg gagccagttc ctcatcgacg ccgagggcta cgaccgggtc cgcggcaagc
     2461 cgttcacgat cgccga
//
LOCUS       DATAIW010000437         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_281879, whole genome shotgun sequence.
ACCESSION   DATAIW010000437 DATAIW010000000
VERSION     DATAIW010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281879"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VHM48_13125"
     CDS             complement(<1..463)
                     /locus_tag="VHM48_13125"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX2756405.1"
                     /translation="MAASVAGSGVAPGRALRLAHRGDWRGNVENTLPAFLAALAVRGC
                     DGLEFDVRAARGGVAVCYHDDTLTRVHGVDRRIDEMTPDELELLGVPTLEAVLTTISR
                     SAFLDVELKGDPASVALSQRGQSGASPAIRSSAAGSNDETTARSRPGPRPAT"
     gene            complement(466..1212)
                     /locus_tag="VHM48_13130"
     CDS             complement(466..1212)
                     /locus_tag="VHM48_13130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2756406.1"
                     /translation="MDRLHIATLNIRNLADRWDERMPLILADMAALQPDVVGLQEVVY
                     VLQQDRLIGSAGVGRYESTRGWAGRPEYGNALLVKEPLVAADAERLDLGLTRSAIRAH
                     LVLAGGASVSVVVAHLHHVTADEAERDEQARILVDWIAAAPPADGIVVVGDFNAEPVE
                     ATYTRMRTAGYRSAYFEANRAEPDVTWPSGLAAPAIDADGPPGCLDYIWIHGAIAVES
                     CRLVFDRPAVGDPGLYPSDHLGLAARLTVG"
     gene            complement(1309..2772)
                     /locus_tag="VHM48_13135"
     CDS             complement(1309..2772)
                     /locus_tag="VHM48_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756407.1"
                     /translation="MDWFVVGVIALLTGAVVLAGWVARRRGQELNETRAALARPSPRA
                     TDPAGATIGPGETGADPARPLDSEVVDAGVDLPALARRLRERLDATQFELGEQARNAS
                     YLADLMGVGIIRLDERGTVEVANAAAHIFLQRPTGSLRGRTALEAFLDGRVEELIAAA
                     RAGDGASGEFRLSGVDGPTLAIRARRSPIGGVWVVLEDVSELRRLQQIRTQFVDNLSH
                     ELRTPLSTVSLLAETLARDAEAAGDGVPPRMRDRIGKIEVETGHLVQMVNELLDLARI
                     EGGGPLVLLDDVDLGRIAADASERLRLFAERQGLRLVVDVPPRVPPIRGDEARLGQVV
                     VNLVHNALKFGRAEDRLGETPGSLWAATAAVGPGGEPAGIADPAGIAVPAPEQAAAEV
                     RVTVRAADGEVLLSVEDHGVGISAADRVRIFERFYKVDRVRVRGGGTGLGLAIARHVV
                     EQHGGRIQVESQEGRGSTFTVILPAAPAPGSGAVGEG"
     gene            complement(2919..3872)
                     /locus_tag="VHM48_13140"
     CDS             complement(2919..3872)
                     /locus_tag="VHM48_13140"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX2756408.1"
                     /translation="MNRHRHIPLVTVLLAGSALVGACGGSSGPLGSVPIASASAGPSV
                     ALGSPDATPSEEPSTGPSEEPTGSTNPGASTTPTPAPSGTTIVRAYFWLSGLPGSEGP
                     VAVLRTIPATKAVATAAVSALLAGPTTAETGRSITTAIPGGTQLLDLAIDKSVATVNL
                     SAEFVGGGDSAAVQTRLAQVTYTLTQFPTVKSVIFQIEGVQLADAVTRATYVALLPDI
                     WVDRPAFNAAIGNPAHVTGNANVFEATFRMSILDASGKVIADVQVMATCGTGCPGTFD
                     TTIPYTVSKGQYGILRAYNPSAKDGTPESIRDYRVWLTPKG"
     gene            complement(3898..>4571)
                     /locus_tag="VHM48_13145"
     CDS             complement(3898..>4571)
                     /locus_tag="VHM48_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2756409.1"
                     /translation="HELRTPVAALVAEASLLDGHLDALPPETRRTGELVVHDIGRLRS
                     LVEDLMELSRFDAAGEEVRLQPIDLGRLIRDVATARLRDAVLELPDDRIVVETDPRRL
                     ERILGNLLDNAREHAPGALVVVRLRATVDEIRIAVLDRGPGVPPDRLGRIFERFYMAD
                     PSRRGGSGLGLAIAAEHATLLGGRLRAINRSDGGLEIELILPVTEQLPAGDPAAISRD
                     DAEDR"
BASE COUNT          639 a         1612 c         1698 g          622 t
ORIGIN      
        1 tcgtcgcggg tcgaggcccg ggtctcgagc gggcggtcgt ttcgtcgttc gagccggccg
       61 cgctcgagcg gatcgccgga ctcgccccgc tctggccgcg ctggctcaac gcgaccgacg
      121 cagggtcccc cttgagctcg acgtcgagga atgcgcttcg cgagatcgtg gtgaggaccg
      181 cctcgagggt cggcaccccg agcagctcga gctcatcggg tgtcatctcg tcgatccgtc
      241 gatcgacgcc gtggacacgt gtcagcgtgt catcgtggta gcacacggcc acgccgccgc
      301 gggccgcccg gacgtcgaac tcgagaccgt cgcagccccg gaccgcgagc gcggcgagga
      361 atgcgggcag cgtgttctcg acgttgccgc gccagtcgcc gcgatgggcg agccgcaggg
      421 cgcgaccggg cgcgaccccg gatccggcga cggaggcggc catcctcaac cgacggtcag
      481 gcgcgccgcg agcccgagat gatcggacgg atagagcccc gggtccccga cggccgggcg
      541 atcgaacacg aggcggcagg attcgaccgc gatcgcgccg tggatccaga tgtagtcgag
      601 gcagccgggc ggcccgtcgg cgtcgatcgc cggcgccgcc agcccggacg gccaggtgac
      661 gtcgggctcg gcccggttcg cctcgaagta cgccgagcgg tacccggcgg tccgcatccg
      721 ggtgtacgtc gcctcgaccg gctccgcgtt gaagtcgccg acgaccacga tcccatccgc
      781 cgggggcgca gcagcgatcc agtcgaccag gatccgggcc tgctcgtctc gctccgcctc
      841 gtcggccgtg acgtggtgga ggtgcgccac gacgacgctc acgctcgcgc cgccggcgag
      901 gacgagatgc gcccggatcg ccgaccgggt cagtccgagg tcgagtcgct cggcgtccgc
      961 tgcgaccagc ggctccttca cgagcagcgc gttgccgtac tccgggcgac cggcccagcc
     1021 acgggtcgat tcgtagcggc ccaccccggc cgatccgatc aggcggtcct gctggaggac
     1081 gtagacgacc tcctggagcc cgacgacatc gggctggagg gcggccatgt cggccaggat
     1141 cagcggcatg cgctcatccc agcgatcggc caggttgcgg atgttgagcg tcgcgatatg
     1201 caggcggtcc atggcaccaa gtgtgaccgt tccgccgggc cgagtgaagc ctcggtttcg
     1261 atccatggcg agtcgtggcg ccgaatgttc ggtcgggtcg cggcgcgctc agccttcgcc
     1321 gaccgcgccc gaaccgggcg ctggcgccgc gggcaggatc accgtgaacg tcgacccccg
     1381 gccctcctgg gattcgacct ggatgcgccc gccgtgctgc tcgacgacat gacgggcaat
     1441 ggcgagtccg aggccggtcc caccgccgcg gacccggacc cgatcgacct tgtagaagcg
     1501 ctcgaagatc cgcacccggt cggccgccga gatcccgacg ccgtgatcct cgaccgagag
     1561 gagaacctcc ccgtcggccg cccggaccgt cacgcggacc tcggctgcgg cctgctcggg
     1621 ggccgggaca gcgatcccgg ccgggtcagc gatcccggcc ggctcgccgc ccggaccgac
     1681 cgcggcggtg gcggcccaca gggagcccgg cgtctcgccg aggcgatcct ccgcccgccc
     1741 gaacttcagg gcgttgtgga cgaggttcac gacgacctgc ccgagccggg cctcgtcgcc
     1801 acggatcggc ggaacccgtg gcggaacgtc gacgacgagg cgcagcccct ggcgttcggc
     1861 gaagagccgg agccgctccg atgcatccgc ggcaatccgg ccgagatcga cgtcgtcgag
     1921 cagcaccagc ggccctccgc cctcgatccg ggcgaggtcg agcagctcgt tgaccatctg
     1981 gacgagatga ccggtctcga cctcgatctt gccgatccgg tcgcgcatcc gcggcggaac
     2041 gccgtccccg gcggcctcgg catcgcgggc cagggtctcg gccagaaggc tgaccgtcga
     2101 gagcggggtc cgcaattcgt gcgagaggtt gtcgacgaac tgggtccgga tctgttgcag
     2161 gcgacgcagc tcggacacgt cctcgaggac gacccacacg ccgccgatcg gtgagcggcg
     2221 ggcccggatc gcgagtgtcg ggccgtccac gccgctcagc cggaactcgc ccgatgcccc
     2281 gtcaccggcc cgggcggcgg cgatcaactc ctcgacccgc ccgtcgagga acgcctcgag
     2341 agcggtccgg ccacggagcg atccggttgg ccgctggagg aagatgtggg ccgcggcgtt
     2401 cgccacctcg accgtgccgc gctcgtcgag gcggatgatc ccgacgccca tcagatcggc
     2461 caggtacgag gcgttgcggg cctgctcccc cagctcgaat tgggtggcgt cgagccgttc
     2521 tcgcaggcgg cgggcgaggg ccggcaggtc cacgccagcg tcgacgacct cagaatcgag
     2581 agggcgcgcc gggtccgcgc cggtctcgcc gggcccgatc gtcgcgcccg ccgggtcggt
     2641 cgctcgcggg ctcgggcggg cgagcgcggc ccgcgtctcg ttgagctcct gcccacgccg
     2701 gcgggcgacc cagcccgcga gcacgaccgc gcccgtgagc agcgcgatca ccccgacgac
     2761 gaaccaatcc atcgtgcgag catggcacac gctcgcccgg cggcgcaccg cggcgcgcag
     2821 cccgaccgat tcaaccggcg gacaaccgga cgccccgggc ggatgcccgg ggcgtcgtcg
     2881 cgatctgatc ccggtcgtcg ggcgaacgaa ggttggcgtc agcccttcgg cgtcagccag
     2941 acccggtagt cgcggatcga ctctggggtc ccgtccttgg cggacgggtt gtacgcccgg
     3001 aggatgccgt actggccctt gctgaccgtg tacgggatcg tcgtgtcgaa cgtcccgggg
     3061 cagccggtgc cgcaggtcgc catgacctgg acgtcggcga tgaccttgcc gctcgcatcg
     3121 aggatcgaca tccggaacgt cgcctcgaag acgttggcgt tgccggtcac gtgggcaggg
     3181 ttgccgatcg cggcgttgaa tgccggccgg tcgacccaga tgtcgggcag gagcgccacg
     3241 tacgtcgctc gggtcacggc atcggccagt tgaacgccct cgatctggaa gatcacggac
     3301 ttcacggtcg ggaactgggt cagggtgtag gtcacctggg ccaatcgggt ctggaccgcg
     3361 gcgctgtcac cgccgccgac gaattcggcc gacaggttga cggtggcgac gctcttgtcg
     3421 atcgcgaggt cgaggagctg ggtgccgcct gggatcgccg tcgtgatcga gcggccggtc
     3481 tcggcggtcg tcggcccggc gaggagcgcg ctgacggccg cggtcgcgac cgccttcgtg
     3541 gcggggatcg tgcgaagcac ggcgaccggg ccctcactgc ccggcaggcc gcttagccag
     3601 aagtaggccc ggacgatcgt cgtgcccgac ggagccgggg tcggggtggt gctggcgccg
     3661 gggttggtcg agccggtcgg ctcctccgag ggtcccgtcg agggctcctc ggacggcgtg
     3721 gcgtcgggac tgcccagggc gaccgacggc ccggccgagg ccgaggcgat cggcacgctg
     3781 ccgagcgggc cgctgctgcc gccgcaggcg ccaaccaggg cggacccggc gagcagaacg
     3841 gtgacgaggg ggatgtggcg gtggcggttc atggtcgaac cctccgtgtg gtgcggttca
     3901 tcgatcctca gcatcgtccc gggagatcgc cgccggatcg ccagcgggta actgttccgt
     3961 cacgggcagg atgagctcga tctcgagccc gccgtcgctt cggttgatcg cgcggagccg
     4021 gccgccgagg agagtcgcat gctctgcggc gatcgcaaga ccgaggccgc tgccgccgcg
     4081 ccggctcggg tcggccatgt agaaccgctc gaagatccgt ccgagacgat cgggcgggac
     4141 cccggggccg cgatcgagga cggcgatccg gatctcgtcg acggtcgccc gcagccggac
     4201 gacgacgagc gctcccgggg cgtgctcgcg ggcgttgtcg agcaggttgc cgaggatccg
     4261 ctcgagccgg cgggggtccg tctcgaccac gatccgatcg tccggcagct cgaggacggc
     4321 atcccgcagg cgagcggtcg cgacgtcgcg gatcaggcgg ccgagatcga tcggctggag
     4381 gcggacctcc tcgcccgccg cgtcgaaccg cgacagctcc atcaggtcct cgacgagcga
     4441 gcgcaagcgg ccgatgtcgt ggacgaccag ctcgccggtc cggcgcgtct ccggcgggag
     4501 ggcatcgaga tggccgtcca ggagtgacgc ctcggcgacg agggcggcga ccggcgtccg
     4561 cagctcgtgg g
//
LOCUS       DATAIW010000438         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_193264, whole genome shotgun sequence.
ACCESSION   DATAIW010000438 DATAIW010000000
VERSION     DATAIW010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193264"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1532)
                     /gene="selA"
                     /locus_tag="VHM48_13150"
     CDS             complement(162..1532)
                     /gene="selA"
                     /locus_tag="VHM48_13150"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX2756410.1"
                     /translation="MSSQPVGRPRPPSVERVLNAVKARPGPERSHDVLVEMARTVIAD
                     ERRRIAGGAGGRPVDDLVDDVLAGLDAHITSAVDTVINATGVIIHTNLGRAPWPSVAG
                     EMAGGAVDYLLLEIDRETGRRGPRFRLAEEHLIALTGADDALVTNNNAAALALAVGLA
                     RRGGIVVSRGELVEIGGGVRIPDIVRRAGAKLIEVGTTNRTRAADYEAPLVEGRAHAV
                     LRVHASNFTMAGFTETPDAAEVAEIAHRHEAIVIDDLGSGALLPTERFGLVHEPMPRE
                     RLAAGSDIVTFSGDKLVGGPQAGLVVGRADLIARMRRDPLARAMRPDKATLLGVAATL
                     GLYRAGLAESQIPVWVTMAVPVVALRQRAEQVVEWIGDRAIVVELRSAVGGGSLPGQT
                     LPSIGVAIQGGSADRVLAALRRRGRTALIGRIADGKVVLDLRTVAPERDRELASIVEA
                     VLGGRP"
     gene            complement(1552..1648)
                     /locus_tag="VHM48_13155"
     tRNA            complement(1552..1648)
                     /locus_tag="VHM48_13155"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1612..1614),aa:Sec,seq:tca)
     gene            1824..2882
                     /gene="selD"
                     /locus_tag="VHM48_13160"
     CDS             1824..2882
                     /gene="selD"
                     /locus_tag="VHM48_13160"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX2756411.1"
                     /translation="MPPIRLTELTDCGGCAAKLGADLLADALSGLGAAGPEPAELLAG
                     LERADDAAVYRISAELAIIGTLDFFPPLVDDARTFGEIAAANALSDVFAMGGRVLFAL
                     SIAAFPEELPRDTMAAIFDGAAAKVREAGGTLAGGHTIRDPEPKYGLAVIGAAHPDRL
                     LRKGGARPGDTLLLTKRLGTGLLVSGHREGKTAAADLVAAVDQMRTLNRAASEVLVAR
                     GVSAATDVTGFGLLGHGFEMARGSGTRFVFEAACLPALGGALELAAAGVETGGTAHNR
                     RFVGPVLSVADGVTPELVALAHDPQTSGGLLAAIAPERLAAVRSGLADAGVEHWLVGR
                     VEAIDGGVPGGVPGVALA"
     gene            2879..>3060
                     /locus_tag="VHM48_13165"
     CDS             2879..>3060
                     /locus_tag="VHM48_13165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2756412.1"
                     /translation="MIVALIPGYNEAPRIGAVVEAAHRLLPVLVVDDGSTDATGDEAR
                     AAGANVIEQRPNQGKGA"
BASE COUNT          398 a         1117 c         1131 g          414 t
ORIGIN      
        1 gagctccgtc ccgtcgggca gggtcaggtg ggcgtacccg acgtcgatcg tcatgccccg
       61 ggcgcgctcg tcgggcagcc ggtcggcgtc gatcccggtc agggcccgca gcagcgtcgt
      121 cttgccatgg tcgatatggc ccgcggtccc gacgacgacg ctcacggccg gccgccgagg
      181 acggcctcga cgatcgaggc gagttcgcgg tcgcgctccg gtgcgacggt ccgcaggtcg
      241 aggacgacct tcccgtcggc gatccgcccg atcagcgccg tccgcccgcg gcgtcggagg
      301 gccgccagca ctcggtcagc ggagccgccc tggatcgcga cgccaatcga aggcaacgtc
      361 tggccgggca ggcttccccc gccgaccgcc gacctgagct cgaccacgat ggcccggtcc
      421 ccgatccact cgacgacctg ctccgctcgc tgccgcaggg caacgaccgg gacggccatc
      481 gtcacccaga ccgggatctg gctctcggcc agaccggcgc ggtagagacc gagggtggcg
      541 gcgacgccga ggagcgtggc cttgtccggt cgcatcgccc gggccagtgg atcgcgccgc
      601 atccgagcga tcaggtccgc ccggccgacg acgagcccgg cctgcggccc gccgaccagc
      661 ttgtcgccgc tgaacgtgac gatgtcggac cccgcggcga ggcgttcgcg cggcatcggc
      721 tcgtgaacca gcccgaaccg ctccgttggc aggagcgccc ctgacccgag gtcgtcgatg
      781 acgatcgcct cgtggcggtg ggcgatctcc gcgacctcgg ccgcgtccgg cgtctcggtg
      841 aagcccgcca tcgtgaaatt ggatgcgtga acgcgaagga cggcgtgggc acggccttcg
      901 acgagcggcg cttcgtaatc cgccgcgcgg gtccggttcg tcgtcccgac ctcgatgagc
      961 ttcgccccgg cgcgccggac gatgtccggg atccgcacgc cgccgccgat ctcgacgagc
     1021 tcgccacgcg acacgacgat cccgccccgc cgggcgagcc cgacggccag ggcgagggcg
     1081 gccgcgttgt tgttggtcac gagcgcatcg tcggcgccgg tcaacgcgat cagatgttcc
     1141 tcggcgaggc ggaaccgcgg gccgcgtcgt ccggtctcgc ggtcgatctc gagcaacagg
     1201 tagtcgaccg cgccgccggc catctcgccg gccaccgacg gccatggcgc ccgtcccagg
     1261 ttggtgtgga tgatcacgcc ggtggcgttg atgaccgtgt cgacggcgga cgtgatgtgc
     1321 gcgtccaggc cggcaaggac gtcgtcgacg aggtcgtcga ccggacggcc gccggcgccc
     1381 ccggcgatcc tccgtcgctc gtcggcgatc actgtccggg ccatctcgac gaggacgtca
     1441 tggcttcgtt cggggcccgg acgcgccttc acggcgttca ggacgcgctc gacactgggc
     1501 ggacgagggc gaccgaccgg ctgcgacgac atcggacctc cagcgcgagg gtggcgggag
     1561 cgcatgggag tcgaacccac cacgccgcgc cgaacgcagc gtcaccggtg ttgaagaccg
     1621 ggggacccac cgggacccct ccgctccctc gatcgcgatg gtaacgcgag gtgtggcgga
     1681 cgccagttcc cggaggggaa ggcaggccga tgagtttccg gcgcggccga cgtccacatg
     1741 gttggcgccg gacgacaggc ttcgtcgggc gcctccgctt gtccgcctat gatccgccga
     1801 tgacgagcgc cgaggtcccg accctgccgc cgatccgcct gaccgagctc accgactgcg
     1861 gcggctgcgc ggcgaagctc ggcgcggacc tgctcgccga cgccctgagt ggcctcggcg
     1921 ccgcggggcc cgagccagcc gagctgctgg cgggtctcga acgggccgac gacgcggcgg
     1981 tctaccggat cagcgccgag ctcgcgatca tcggcacgct cgacttcttc ccgccgctcg
     2041 tcgacgatgc ccggacgttc ggcgagatcg cggccgcgaa cgcactgagc gacgtgttcg
     2101 cgatgggtgg ccgcgtcctg ttcgccctct cgatcgcggc attcccggag gagttgccgc
     2161 gggacacgat ggcggcgatc ttcgacggcg cggccgccaa ggtccgtgag gccggcggaa
     2221 ccctcgccgg cggccacacg atccgcgatc cggagccgaa gtacgggctg gcggtcatcg
     2281 gcgcggcgca cccggatcgg ctgctgcgca agggtggggc gcggccgggc gacacgctcc
     2341 tcctgaccaa gcgactgggc acgggcctgc tcgtcagcgg ccaccgcgag ggcaagaccg
     2401 cggcggccga tctcgtcgcg gcggtggacc agatgcggac gttgaaccgg gccgcctccg
     2461 aggtgctcgt cgcccgaggg gtctcggcgg cgaccgacgt gaccggcttc ggcctgctcg
     2521 ggcacgggtt cgagatggct cgcggctccg ggacgcggtt cgtcttcgag gcggcctgcc
     2581 ttccggccct cggtggggcg ctcgagctcg cggccgccgg cgtcgagacc ggcggcaccg
     2641 cccacaaccg gcggttcgtc ggacccgtcc tgagcgtggc cgacggggtc acacccgagc
     2701 tcgtcgcgct tgcccacgac ccgcagacct cgggcgggct gctcgccgcc attgcgccag
     2761 agcggctggc cgcggtcagg tccggtcttg ccgacgctgg cgtcgagcac tggctcgtcg
     2821 ggcgggtcga ggcgatcgac ggcggcgtgc ccggcggtgt gcccggcgtc gccctggcgt
     2881 gatcgtcgcc ctgatcccgg gctacaacga ggcaccgcgc atcggcgccg tggtcgaggc
     2941 ggcgcaccgg ctcctgccgg tcctcgtggt cgacgacggc tccacggacg cgaccgggga
     3001 cgaggctcgc gccgcgggcg cgaacgtgat cgagcagcgt ccgaaccagg gcaagggcgc
//
LOCUS       DATAIW010000439         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_252294, whole genome shotgun sequence.
ACCESSION   DATAIW010000439 DATAIW010000000
VERSION     DATAIW010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252294"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..858)
                     /locus_tag="VHM48_13170"
     CDS             complement(139..858)
                     /locus_tag="VHM48_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756413.1"
                     /translation="MGLAAGTDGLIRRSDLQPVATTVAVLDPVSCRVISEIALGAEAH
                     VFVDIDTAGQVDTHPYDPSDYGTWPNIGALLPDAPQCASADPDPSSTAAPTPSVVDGV
                     RLEPGAIDCQIGPEAGTGPDASTEPFDVRVEFVVGAVGGCVARDDDIRPGSADRSAVT
                     IWNPDNDLHRLALAWDGTGCTVSATVSLAPTLAGYRADVISIDVPCNNKKPRPFGVAI
                     SVVDAIDAAGVTTTIDRVEEP"
     gene            complement(1160..>1540)
                     /locus_tag="VHM48_13175"
     CDS             complement(1160..>1540)
                     /locus_tag="VHM48_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756414.1"
                     /translation="SKLYETAIPASVREAIREAMKEAGKPSFWDPPEDATPEQIAEHE
                     KFLAKMLVPDDDITTWVDISGDPIDRKWRAIQRHVTQISMDSGFMVFGEAGWKEHWPR
                     EAYILRESTVETTKPEDDLFAGLL"
BASE COUNT          232 a          522 c          543 g          243 t
ORIGIN      
        1 ccgaacttcc tcgacccgaa gggcgacggc cggatggtga tgggccagat cttcgacgcc
       61 tcgaagggga tgctcccctg gtaaggctgt cgaatgcaat cgcgaagccg cgatgcggct
      121 ttgtgatgcc tcgttcggtc agggctcctc gacccggtcg atcgtggtcg tcacgccggc
      181 agcgtcgatc gcgtcgacca cgctgatcgc gacgccgaat ggcctgggct tcttgttatt
      241 gcacggaacg tcgatcgaga tcacgtcggc gcggtagccg gcgagggtcg gtgccaggct
      301 gacggtcgca ctgacggtgc aacccgtccc gtcccaagcg agggccaggc ggtggaggtc
      361 gttgtcgggg ttccagatcg tgacggcgga tcggtccgcg gagcccggtc ggatgtcgtc
      421 atcgcgggct acgcagccgc cgacagcgcc gacgacgaac tcgacccgga cgtcgaacgg
      481 ctccgtcgac gcgtccggac cagtaccggc ttccggcccg atctggcagt cgattgcccc
      541 cggctcgagc cggacgccat cgacgacgga cggggtcggg gccgccgtag agctcggatc
      601 cgggtcggcg ctcgcgcatt gcggcgcgtc gggcaggagc gcgccgatgt tgggccaggt
      661 gccgtagtcg ctcggatcgt acgggtgggt gtcgacctgg ccggcggtgt cgatgtcaac
      721 gaagacgtgc gcctcggccc cgagcgcgat ctcggagatc acccggcacg acaccggatc
      781 gaggaccgcg acggtcgtcg cgaccggctg gagatcggat cggcggatca acccgtcggt
      841 gccggccgcg aggcccacgt acgagaccgc tccgtccgac gtggcgcgga cgacgaaatc
      901 gatccccgac cacgggttct ccgttcggat cgacgagccc ctcggagcac ggatcaactg
      961 gcagccggtg agtccgagcg acgccgcaag gagcagcgca gcgacccagg tgcctcggcg
     1021 acgcgatcga gcggaacggg acaacatgga gcgcctccgc gaccgggtga cgacgagcgg
     1081 tcgcgcgccg cccgaccgcc cgggctcgga cggctcaccg ccgcgtgccg tgactctgcc
     1141 cgccacggcg cggtgcgcac tacagcagac cagcgaagag gtcgtcctcg ggcttcgtcg
     1201 tctcgaccgt cgactcgcga aggatgtacg cctcccttgg ccagtgctcc ttccagccgg
     1261 cctcgccgaa gaccatgaag ccgctgtcca tggagatctg ggtgacgtgg cgctggatcg
     1321 cccgccactt gcggtcgatc gggtcaccgg agatgtcgac ccaggtcgtg atgtcgtcgt
     1381 ccgggaccag catcttggcg aggaacttct cgtgctcggc gatctgctcc ggcgtcgcgt
     1441 cctccggcgg gtcccagaag ctcggcttgc cggcttcctt catcgcctcg cgtatcgcct
     1501 cgcggaccga cgccgggatc gccgtctcgt agagcttcga
//
LOCUS       DATAIW010000440         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_282858, whole genome shotgun sequence.
ACCESSION   DATAIW010000440 DATAIW010000000
VERSION     DATAIW010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282858"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VHM48_13180"
     CDS             complement(<1..277)
                     /locus_tag="VHM48_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756415.1"
                     /translation="MTTNDAERERLVEIVHRLTPREHILEAMPGWTIGSLLAHLPFWD
                     RLVIERWTRATADGAAAPPGLSEALTDIINAASQAQWWALPGPVAGAE"
     gene            complement(366..1658)
                     /locus_tag="VHM48_13185"
     CDS             complement(366..1658)
                     /locus_tag="VHM48_13185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756416.1"
                     /translation="MFGRRIPGLWHEGDFVRLWAAATVSMLGSFITRSALPFTAILVL
                     GAGAPEVALIRGAELVAGLTLGLVAGAWIDRRRRRPVMVAADLGRAVLLGSIPLAALG
                     GVLGLPQLVIVAFGAAVLTTFFDVADRAFLPTVVGRDRLVSANSTLTASGSAAEFIGF
                     GAGGWLIQLLTAPIAIALDAVSFVVSAVLLGRIRTHEPAVIVPDGGHEPILDEIREGL
                     RLTFRDPILRPLALGDAAVAAFWGVFGATYFVFATQLGFSPGVIGLVAAVGGASSFIG
                     ALAVNRGVRRLGVGRFLVAALVVVATGNLAIAFAPDASIVGLAFLLVQQLLSDSALTA
                     FDVVAVSIRQTTVDDRALGRVGASMHVLSLAAMLVGTIVGGIVAATISVRAAIFVGGA
                     GGFVAVAVVWFSRVRTLRDMPLPPALPIRAGVDVRLGE"
     gene            2024..3358
                     /gene="ffh"
                     /locus_tag="VHM48_13190"
     CDS             2024..3358
                     /gene="ffh"
                     /locus_tag="VHM48_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX2756417.1"
                     /translation="MFDSLSERLRKTLSDLTGRGRVSEADVDAAMREIRLSLLEADVN
                     FKVVKDFVGRVREQAVGAEILQSLSAGQQIVKIVNDELVALLGAGDRTFHLTGNPAVV
                     AMVGLQGSGKTTSSAKLARHLVRQGRRPLLVAADPYRPAAADQLETLGKALDIPVHRA
                     PVGTSVVDIARGGVEAAHRLARDVVILDTAGRLSVDQALMDEIAAVNAAVKPVETLLV
                     VDAMTGQEAVAVATAFKAAVPLTGLILTKIDGDARGGAALSISAVTGLAVKFMGTGEK
                     TDALEVFHADRLAGRILGMGDVMTLVERAQEHVDAGQAAKLEEKLRKSQFTLEDFLEQ
                     IQQVQKMGPMGQLMGMIPGMGGMAKQAQEAVDRGDLRRVEAIIRAMTPRERRDPAILN
                     GSRRRRIAAGAGTDLTEVNRLIKQHAELSKLMKQLSGGGGRKAAMAGLMGRR"
BASE COUNT          558 a         1207 c         1196 g          404 t
ORIGIN      
        1 cctccgcgcc ggccaccgga ccgggaagcg cccaccactg ggcctgcgac gccgcgttga
       61 tgatgtccgt caatgcctcg ctgaggccgg gtggcgccgc tgctccatcg gccgtggccc
      121 gggtccagcg ctcgatcacg agccggtccc agaacgggag atgagcgagc aacgatccga
      181 tcgtccagcc gggcatcgcc tcgaggatgt gctcccgagg tgtcagccgg tggacgatct
      241 ccacgagccg ttcacgttcc gcgtcattcg tcgtcacgcg accgtcctcc gttgtctgtc
      301 ggcgtcttcc agcacccagc atggcgtggg gacctggagg ccgcgccgtc cgggcatcgc
      361 caccgtcatt cgccgagccg cacgtcgacg cccgccctga tcggtagcgc cggagggagc
      421 ggcatgtcgc gcaacgtccg gacgcgcgag aaccagacga ccgcgacggc cacgaagccg
      481 cccgcgccac cgacgaagat cgccgcccgg acgctgatgg tcgccgcgac gatcccgccg
      541 acgatcgttc cgacaagcat ggccgccagc gacaggacgt gcatgctcgc gccgacccgg
      601 ccgagcgccc ggtcgtcgac ggtcgtctgg cggatcgaga ccgcgacgac gtcgaaggcg
      661 gtcagggcac tgtccgagag cagctgctgg acgagcagga acgccagccc gacgatcgat
      721 gcgtccggcg caaaggcgat cgcgaggttg cccgtcgcga ccaccaccag ggccgcgacg
      781 aggaaccgcc cgacgccgag ccggcggacg ccgcggttca ccgcgagggc cccgatgaac
      841 gagctggccc caccgaccgc ggccacgagc ccgatgacgc cgggcgagaa cccgagctgg
      901 gtcgcgaaga cgaaataggt cgcgccgaac acgccccaga acgcggcaac cgcggcgtcg
      961 ccgagggcga gcgggcgaag gatcgggtcg cggaaggtca gccgcagacc ctcccggatc
     1021 tcgtcgagga tcggctcatg gccgccgtca ggcacgatca ccgcgggttc gtgggtccgg
     1081 atccgcccga gcaggaccgc cgacacgacg aatgagaccg catcgagggc gatcgcgatc
     1141 ggggcggtca gcagctggat cagccagccg ccggcaccga acccgatgaa ctcggcggcg
     1201 gatccactgg cggtcagggt cgagttggcg gacaccaggc ggtcgcgacc gacgaccgtc
     1261 ggcaggaacg cccggtcggc gacgtcgaag aaggtcgtca ggaccgccgc gccgaaggcg
     1321 acgatgacga gctgcggcag cccgagcacc ccgccgagcg ccgcgagcgg gatgctcccg
     1381 agcaggacag cccgtccgag gtcggccgcg accatcaccg gccgccgccg tcgccgatcg
     1441 atccaggcgc cggcgacgag gccgagcgtc aacccggcga ccagctccgc gccgcggatc
     1501 agggcgacct cgggggctcc ggcgccgagg acgaggatcg cggtgaacgg cagcgccgag
     1561 cgggtgatga aggagccgag catcgagacc gtcgcggccg cccacagccg gacgaaatcg
     1621 ccctcgtgcc agagaccggg gatgcgtcgt ccgaacatcg ggcaagccta atcggccggg
     1681 agagggccgc cgcgtggcgc ggcgaggtct cgtcacgggg cgcagactga tcgggccgct
     1741 cattgactcg ctcgctgagt cagattccgg ctcagcatgc agagctcggt cgcgacccgg
     1801 acccgggcga gcgtgctgcg ggcggaattg actcaatggc cgagtcggcg ggagccggcc
     1861 ccgagcaacg gcaacgacag ccgtcgcctg ccgtgcgggt cgcgccgggc catgcgctac
     1921 actttccggg cctcggtgcg ccagaactcg tgcaccaccc gatcgtcgtc gccggcgcgg
     1981 cacgccgttc ggtccacttc cacgctggag tcccacccga accgtgttcg acagcctgag
     2041 cgagcgcctg cgcaagaccc tgtccgacct gaccggccgc ggccgcgtca gtgaggcgga
     2101 cgtcgatgcc gcgatgcgcg agatccggct cagcctcctc gaggcggacg tcaacttcaa
     2161 ggtcgtcaag gatttcgtcg ggcgcgtccg ggagcaggcc gtcggcgccg agatcctgca
     2221 gagcctgagc gccggccagc agatcgtgaa gatcgtcaac gacgagctgg tcgccctgct
     2281 cggcgccggc gaccggacct tccacctgac cggaaacccg gccgtcgtgg cgatggtcgg
     2341 gctccagggc tcgggcaaga cgacctcatc ggccaagctg gcccgccatc tcgttcgcca
     2401 gggccgccgg ccgctgctcg tcgccgcgga cccctatcga cccgctgccg ccgaccagct
     2461 cgagacgctc ggcaaggcgc tcgacatccc cgtccatcgg gcgccggtcg gaacctcggt
     2521 cgtcgacatc gcccgcggcg gtgtcgaggc agcccaccgc ctcgcccgcg acgtcgtcat
     2581 cctcgatacg gccggccgcc tgagcgtcga ccaggcgctg atggacgaga tcgccgcggt
     2641 caacgccgcg gtgaagcccg tcgagaccct cctcgtcgtc gatgcgatga ccggccagga
     2701 agcggtcgcg gtcgcgaccg cgttcaaggc cgcggtcccg ctgaccggcc tgatcctgac
     2761 caagatcgac ggcgatgccc gcggcggcgc cgcgctctcg atcagcgccg tcaccggcct
     2821 ggccgtcaag ttcatgggga caggcgagaa gaccgacgcg ctcgaggtct tccacgccga
     2881 ccgcctcgcc ggccggatcc tcggcatggg cgacgtcatg accctcgtcg agcgcgccca
     2941 ggagcacgtc gacgccggcc aggccgccaa gctcgaggag aagctccgca agtcgcagtt
     3001 cacgctcgag gactttctcg agcagatcca gcaggtccag aagatgggtc cgatgggcca
     3061 gctgatgggg atgatcccgg gcatgggcgg aatggccaag caggcccagg aggccgtcga
     3121 tcggggcgac ctgcggcggg tcgaggccat catccgggcg atgacgccgc gcgagcgacg
     3181 cgacccggcg atcctcaacg gctcgcgtcg gcgccggatc gcggccggtg ccgggaccga
     3241 cctgaccgag gtcaaccgac tgatcaagca gcacgccgag ttgtcgaagc tgatgaagca
     3301 gctcagcggc ggtggtggcc gcaaggccgc gatggccggc ctgatgggcc ggcggtaagg
     3361 ggagg
//
LOCUS       DATAIW010000441         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_223671, whole genome shotgun sequence.
ACCESSION   DATAIW010000441 DATAIW010000000
VERSION     DATAIW010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223671"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1424)
                     /locus_tag="VHM48_13195"
     CDS             complement(156..1424)
                     /locus_tag="VHM48_13195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX2756418.1"
                     /translation="MSRTLRRVWITGIGPITAIGTGRDAFRAGLRAGVSPVRRIDRFD
                     PSPFRSQVAAQIDDFDPAAWMPPKTARQLDGFSQFGLVAGRLALDDAGLRPGEDGGAA
                     AERIGIYLGSALGGIAYAEEQHERYLERGIKAVAPNLALAVFGGAAPANLGIALGVRG
                     PILSTANSCASGAVAIGEALGAIRAGDIDAAIAGGAEVPLSPLAFGAFDIIRALSDSR
                     NDDAAHACRPFDAGRDGFVMGEGATLLVLEAEDVARARGAEPFAEVMGYAATSDAFHM
                     VQPRADGREAGRAAVEALADAGVGPDEIDWVSAHASSTPLGDIAEARAIALALGERAA
                     TVPVSGTKALYGHPLGASGAIEAAICALAIRDGWVPGSANLEVVDPDVGALLPGLLRE
                     GRKGTYRRILSTSFGFGGLNAGLVMGAIDG"
     gene            complement(1421..1924)
                     /locus_tag="VHM48_13200"
     CDS             complement(1421..1924)
                     /locus_tag="VHM48_13200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2756419.1"
                     /translation="MRSTIGIDIAAPADLVFRLARDVERWPDLLPHYVAARRAGAADE
                     AGRLVVRFVARRPLLPVLGLGLPVAWRSLTWSEPVERRLRFVHRGGATGGMDVTWRID
                     DRPGGTCRVDIEHDFRPRVPGWAAFIDRLFTRPIAGRTLATLRVLAEAVAAVDRSVEP
                     PLTYPPA"
     gene            complement(1921..2424)
                     /locus_tag="VHM48_13205"
     CDS             complement(1921..2424)
                     /locus_tag="VHM48_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2756420.1"
                     /translation="MSAVDRPESGLTPGDPEGQDHPPSARTRLGSYAVSVDGDGRILL
                     CRLSADEVEAGAWTLPGGGVEYGEHPDDAVLRELQEEAGLIGRIDDVLGIFSKVYPHS
                     RAAGGADLHFVGLLYRITPIGGALRDEIDGSTDTCAWFRPDELRDLRIVGVARHAIEL
                     ALPGALG"
     gene            complement(2421..>3211)
                     /locus_tag="VHM48_13210"
     CDS             complement(2421..>3211)
                     /locus_tag="VHM48_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2756421.1"
                     /translation="TATLAGGGTLSAHVVVGADGLRSVVARDASVARRPPLGGRTALT
                     FHVADPDPDAVRDARMVVFDGGYVGLAPVPGGRVNVGIVLASGRWRARLRAEGAAAVA
                     RAVMAAIPAASDDPVSWSEPEIRDSIEGAAPVGARVARRAGRDWLLVGDAAGFLDPFT
                     GEGLQRALRSAALAVDAVDHHLGGETGALAAYDRSLARRTRSKDVVSLLVQAFLELPA
                     AFDYAARRLATRPAVRETMGLVMGDLVPASRAFDPRFLLGLLRP"
BASE COUNT          471 a         1219 c         1154 g          367 t
ORIGIN      
        1 gccctgcgtg gctggaacca cacgcgctcg ttgttggcgg cgagctcggc gaggaagtcg
       61 acggcctcgg gacgaaagcc tgtgaacggg gcggatgcga cggccatggc gtggtctcct
      121 gaacgcggcg tgccgtcgat cgtacgcccg ccggcttatc cgtcgattgc gcccatgacg
      181 agcccggcat tcagaccgcc gaagccgaac gacgtcgaca ggatccgccg gtaggttccc
      241 ttccggccct cccgcaagag acccggcagc aacgcgccga catcgggatc gacgacctcg
      301 aggttggcgg atccgggcac ccagccgtcg cggatcgcga gagcgcagat cgctgcctcg
      361 atcgcgcccg aggcgcccaa tggatgcccg tacagcgcct tcgttccgct cacgggcacc
      421 gtggccgccc gctcgccgag ggcgagcgcg atcgcccgag cctccgcgat gtcgccgagc
      481 ggagtcgacg aggcatgggc gctgacccag tcgatctcgt ccggcccgac tccggcgtcg
      541 gcgagcgcct ccacggcggc tcgccccgcc tcgcgaccgt cggcccgcgg ttggaccatg
      601 tggaacgcat ccgaggtcgc cgcatagccc atgacctcgg cgaacggctc ggcgccgcgc
      661 gcccgggcga cgtcctcggc ctcgagcacc aggagcgtcg caccctcgcc catgacgaag
      721 ccgtcacggc ccgcgtcgaa cggccggcag gcatgggccg catcgtcgtt ccggctgtcc
      781 gacagcgccc ggatgatgtc gaacgccccg aacgcgagcg gactcagcgg cacctcggcg
      841 ccgccggcga tcgccgcgtc gatgtcgccc gcccggatcg ccccgagggc ctcgccgatc
      901 gcgaccgcgc ccgaggcgca cgagttggcc gtcgagagga tcggcccacg gaccccgagg
      961 gcgatcccga ggttcgccgg cgccgctcca ccgaacaccg cgagggcgag gttcggcgcg
     1021 accgccttga tcccgcgctc gaggtagcgc tcgtgctgct cctcggcgta agcgatccca
     1081 ccgagggcgc tgccgagata gatcccgatc cgctcggccg ccgcgccgcc gtcctcgcca
     1141 gggcggagac cggcgtcgtc gagggcgagc cggcccgcga cgagcccgaa ctggctgaat
     1201 ccgtcgagct ggcgggctgt cttgggcggc atccaggccg ccgggtcgaa gtcgtcgatc
     1261 tgggcggcga cctggctcct gaacggtgac gggtcgaacc gatcgatccg ccggaccggc
     1321 gacaccccgg ctcgcagccc ggcgcggaac gcgtcgcggc ccgtgccgat ggcggtgatc
     1381 gggccgatcc cggtgatcca gacgcggcga agggtgcggc tcatgctggt gggtacgtca
     1441 acgggggctc gacggaccga tcgacggccg cgaccgcctc cgccaggacg cgcaacgtgg
     1501 ccagcgtccg gccggcgatc ggccgggtga acaggcgatc gatgaacgcg gcccagccgg
     1561 ggacgcgcgg ccggaagtcg tgctcgatgt cgacccggca cgtgcccccg gggcgatcgt
     1621 cgatgcgcca cgtcacgtcc atcccgccgg tcgcgccacc gcggtggacg aagcgcaacc
     1681 ggcgctcgac cggctccgac cacgtcagcg aacgccaggc gaccggcaag ccgaggccga
     1741 ggaccggcag caacggtcgc cgggcgacga acctgacgac cagccgcccg gcctcgtcgg
     1801 ccgcgccggc ccgccgtgcc gcgacgtagt ggggcaggag atccggccag cgctcgacat
     1861 cgcgggccag ccggaacacg agatcggccg gagccgcgat gtcgatcccg atcgtgctcc
     1921 tcacccgagc gcgccgggca gggcgagctc gatcgcgtgg cgggcgacgc cgacgatccg
     1981 gaggtctcgc agctcgtccg gtcggaacca ggcgcaggtg tcggtcgagc catcgatctc
     2041 gtcgcggagc gcgccgccga tgggcgtgat ccggtagagc aacccgacga aatggaggtc
     2101 ggccccgccg gccgcccggg agtgagggta gaccttcgag aagatgccga ggacgtcatc
     2161 gatccggccg atcagcccgg cctcttcctg cagctcgcgg aggaccgcgt cgtcgggatg
     2221 ctcgccatat tcgaccccac cgccgggcag cgtccatgcg ccggcctcga cctcgtcggc
     2281 cgacaaccga cacagcagga tccgaccgtc gccgtcgacg gagaccgcat acgacccgag
     2341 ccgcgtccgc gctgacggcg ggtgatcctg gccctccgga tcgcccggcg tcaggccgga
     2401 ctcaggacga tcgacggcgc tcacggccgc aggagcccga gcaggaagcg cggatcgaag
     2461 gctctcgagg ccggcaccag gtcgcccatc acgaggccca tcgtctcacg gaccgcggga
     2521 cgggtcgcga ggcggcgagc cgcgtagtcg aacgcggccg gcagctcgag gaatgcctgg
     2581 accaggaggc tgacgacgtc cttcgagcgc gtccgtcggg cgaggctccg atcgtaggca
     2641 gcgagcgcgc ctgtctcacc cccgaggtgg tggtccaccg cgtcgacggc cagcgccgcc
     2701 gaccgcaggg cacgctggag gccctcgccc gtgaacgggt cgagaaagcc tgccgcgtcg
     2761 ccgaccagga gccaatcccg gccggctcgc cgtgcgacac gggccccgac cggggccgcg
     2821 ccctcgatcg agtcgcggat ctccggctcc gaccaggaca ccggatcgtc gctcgccgcc
     2881 gggatcgcgg ccatcacggc ccgggcgacc gcggccgcgc cctcggcccg cagccgcgcc
     2941 cgccagcgcc cgctcgcgag cacgatcccg acgttgaccc gtccgcccgg gaccggcgcc
     3001 agcccgacat agccgccgtc gaacacgacc atccgcgcgt cgcggaccgc gtcggggtcg
     3061 ggatcggcga cgtggaacgt cagcgccgtg cggccgccga gcggcggccg ccgcgcgacg
     3121 ctcgcatcgc gcgcgacgac cgagcgcagc ccgtcggccc cgacgaccac gtgcgcgctg
     3181 agggtgccgc cgcccgccag cgtcgcggtt c
//
LOCUS       DATAIW010000442         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_283823, whole genome shotgun sequence.
ACCESSION   DATAIW010000442 DATAIW010000000
VERSION     DATAIW010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283823"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1707)
                     /locus_tag="VHM48_13215"
     CDS             complement(283..1707)
                     /locus_tag="VHM48_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX2756422.1"
                     /translation="MTGSTDPLFDQRIADWLEDDPRHAPGQVLEIVLAALPSISRRRA
                     SRVPRWFTSGRASVRWSVAAAAIVGVLAVGGALSLLRPGQSVVGGPSPTPVPSAGPSE
                     PGPTPTSRPSPTGLLGTGRQIHTATLLADGRVLVAGGFDNGDGNLASADLYDPRTDTF
                     RPTGSLASARGLHTATLLSDGRVLIAGGGVGSWVAAGATYLASAELYDPNTGTFSPTG
                     SMTTSREDHTATRLADGRVLIVGGNDAGSHAVASAELYDPKTGTFSPTGSMAMARGFH
                     TATLLADGRVLIAGGDQAAWTDSGPFLASAEVYDPRTGSFTTTGTMPDGRAYHAATLL
                     SDGRVLITGGVTFGGANPSLASVELYDPRTGKFSPTGAMTVGRVYQTATLLSDGRVLI
                     AGGLAHGRVYANNPQFLDSAELYDPKVGKFSPTGSMTDARTYHGATLLADGRVLVTAG
                     YGDVAPLASAEIYDPNTGTFSPAG"
     gene            complement(1704..>2118)
                     /locus_tag="VHM48_13220"
     CDS             complement(1704..>2118)
                     /locus_tag="VHM48_13220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2756423.1"
                     /translation="QALLGVWRDFATLRDLDRFDAWLHRLVVHACYAEARGQRRWAAR
                     IRILQIDPPTSPDVASSVAARDDLDRAFRGLSPEQRAVVVLHHHLGYPLTEIATTLGI
                     PVGTVRSRLHYAVRQLRMVLDAEGRARATSEERLA"
BASE COUNT          318 a          685 c          818 g          297 t
ORIGIN      
        1 gaggccatcg ccgcgcccaa cgtgagggtc atgagcgcgg cgccggccag gagcgcgagc
       61 gcacgagtgc ggatgcccat gaccccggtc ggggtgccaa ttcgaaccgt gttcattgcg
      121 ggtctcccag gttgcggtgc accgcgggcc aggttcgcca cagacgcgga tcgtcggcgt
      181 cgtggctacc ctccgcccgc gctggtcagt cctgacgcgg catggccctc cttcgggatg
      241 gcattcggtc tcccctgggg ttggagtcgt cgttgacgga cgtcagccgg ccgggctgaa
      301 cgtgccggtg ttcgggtcgt agatctcggc cgaggcgagc ggagccacgt cgccataacc
      361 tgcggtcacg aggacgcggc cgtcggcgag caaggtggct ccgtggtagg tccgcgcgtc
      421 ggtcatcgag ccggtcgggc tgaacttgcc cactttcggg tcgtacagct cggccgagtc
      481 gaggaactga gggttgttcg cgtacacccg gccgtgggcc aagcctccag cgatcaggac
      541 gcgaccgtcg gagagcaggg tggcggtttg gtagacgcgc ccgacggtca tcgcgccggt
      601 cgggctgaac ttgcccgtcc tcgggtcata caactcgacc gacgcgagac tggggttggc
      661 gccgccgaag gtgacgccgc cggtgatcag gacacgaccg tcggagagca gggtcgccgc
      721 gtgataggcg cgcccgtcag gcatcgtgcc ggtcgtggtg aagctgccgg tcctcgggtc
      781 gtagacctcg gccgaggcga ggaacgggcc gctgtcagtc caggcggcct ggtcgccgcc
      841 ggcgatcagg acgcggccgt cggcgagcag ggtggcggtg tgaaaaccgc gggccatcgc
      901 catcgagcca gtcgggctga acgtgccggt cttcgggtcg tacagctcgg ccgaggcgac
      961 ggcatgggag ccggcgtcgt tgcctccgac gatcaggacg cgaccgtcgg caagccgggt
     1021 cgcggtgtgg tcctcgcgac tggtcgtcat cgagccggtc gggctgaacg tgccggtgtt
     1081 cgggtcgtac agctcggccg aggcgaggta ggtggcgccg gcggcaaccc agctcccgac
     1141 tccacctccg gcgatcagga cgcgaccgtc ggagagcaga gttgcggtgt gcaggccgcg
     1201 agccgacgcg agcgagccgg tcggcctgaa cgtgtcagtc cgagggtcgt agaggtcggc
     1261 tgacgcaaga ttcccatccc cgttgtcgaa gcccccggcg acgaggaccc gaccgtcggc
     1321 gagcagcgtg gccgtgtgga tctgtcgccc ggtacccagc agtccggtcg ggctggggcg
     1381 agacgtcggc gtcgggcctg gctcgctcgg accggcgctc ggcacgggtg tcgggctcgg
     1441 gccgccgacc acggactgac cgggccggag cagcgacagc gcgccaccaa ccgcgagcac
     1501 gccgacgatc gccgccgcgg cgacagacca gcggacggac gctcgcccgc tggtgaacca
     1561 ccgcggcacg cgagaagcgc gcctccgcga gatcgagggg agggcggcca ggacgatctc
     1621 gagcacctgg ccgggggcat gccgtgggtc gtcttcaagc cagtcggcga tgcgctggtc
     1681 gaagagcgga tcggtcgatc cggtcatgcc aatcgctcct cagaggtcgc ccgggcgcga
     1741 ccctcagcgt cgagcaccat ccgcagctgg cgaaccgcgt agtgaaggcg ggaccggacc
     1801 gtcccgacgg ggatcccgag ggtggtcgcg atctcggtca gcggataccc gaggtggtga
     1861 tggaggacga cgaccgcgcg ctgttccggt gacagcccgc ggaaggcgcg gtcgaggtcg
     1921 tctcgcgcgg cgaccgagct cgcgacatcc ggcgatgtcg gcggatcgat ctggagaatc
     1981 cggatccggg cggcccagcg gcgctggccg cgggcctccg cgtagcaggc atggacgacg
     2041 agacggtgga gccaggcgtc gaaacggtcg aggtcgcgga gggtggcgaa atcgcgccag
     2101 acgccaagca gggcctgc
//
LOCUS       DATAIW010000443         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_134629, whole genome shotgun sequence.
ACCESSION   DATAIW010000443 DATAIW010000000
VERSION     DATAIW010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134629"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHM48_13225"
     CDS             <1..210
                     /locus_tag="VHM48_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756424.1"
                     /translation="LFPEGGLSLDEFQYVAETDEDANPGQTSIPGVYVAGAASGAKDI
                     ADSILHAGSTVAQVAAWLEKMKVPA"
     gene            207..1949
                     /locus_tag="VHM48_13230"
     CDS             207..1949
                     /locus_tag="VHM48_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEX2756425.1"
                     /translation="MTEQERRIGVYVCHCGGNISDYVDVDQVVAAAQGEPGVVVARQM
                     MFACSDSGQGEMERDIEEQHLDGLVVASCSPKLHVVTFRGVAKRAGLNPYEYTQVNVR
                     EQASWIHSDDAQGTTDKAIHLVKAGIARTLLTEPLEPIVVETTQRTLVVGGGVAGLRA
                     AIGLADIGLGVFLVERTAVLGGWVGTFGAMYPTGKDGKQLIAELLEGIRRRPSIRVFT
                     EAEMVSKSGSFGNYEVGIQVGGSNPETLSVEVGSIVVTTGVDTYAPEVGEFGYGIDGV
                     VTLPEFKTLIDASGGPLVRNGRPVRTVAYIYCVGSREPAGEPGANEYCSRYCCAATVA
                     ASVQVASRDATIHQYHVYRDMRTYGKFETMYTESRKLGSVYLKFADDAPPTVTRTDAG
                     GLLVTVKDVLTGGEELAIPADLVVLVTGMIPRRNEELIGVLKLPVDKDGFFNEIHPKL
                     RPVETVVDGVFIAGACQGPKNSSESVASGLAAVTQSAVILKKGFAELDPLVAIVHADA
                     CTACNLCLEACPYGAIAMGALGDRSVAVISETGCKGCGGCVPVCPENAIDLLGYTDGQ
                     ITAMIDKMLEVSVA"
     gene            1946..2194
                     /locus_tag="VHM48_13235"
     CDS             1946..2194
                     /locus_tag="VHM48_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756426.1"
                     /translation="MNAPNRELREVIREEPIMRARILELLDPEPRTVPELAAAMDCPT
                     HEVLFWIMGMRRYGRVREVKGVTDEGYFRYEAIAQDPS"
     gene            2191..3384
                     /locus_tag="VHM48_13240"
     CDS             2191..3384
                     /locus_tag="VHM48_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2756427.1"
                     /translation="MTALVDTNLLSDLQRYGAADVSACFSCGTCTATCPLVSNDSTFP
                     RRMIRYAQVGLRDRLLSSKELWTCYACGECSETCPTQAEPSEFMAAARRYAIAGYDRT
                     GLARTMYTRPVIGTVIAVVLAAFFALFMYAAHGPQSAGSLDVFRFIPEELIHNLGIAI
                     MVIVFATGLVGVVTMARAVSREGGVSLRSVVGGRAALRRSAGALWTALAVETLGQRRY
                     RDDCDADAAAQRWYLRRWFLHAATMWGFLGLLAATILDYGLAIVGIKATGTAVPIWYP
                     VRLLGTVAGILLVYGATMLIVQRYRGKERSVRHSTSADWTLLALVWITGVTGFALELA
                     LYLPSAPAWGYWVFLFHVAIAMELVILAPFMKLAHAVYRPVALFFLALAGTPAARIDE
                     GGVHG"
     gene            3429..3923
                     /locus_tag="VHM48_13245"
     CDS             3429..3923
                     /locus_tag="VHM48_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX2756428.1"
                     /translation="MTASLPQVSHEHHDRLTRHLDTMPALGDLIGTASVAELRPRVDE
                     IADFLTGLLLPHMEAAEHTLYPELERMLQNRHSMTPMRREHDEIRALVTELGRLQHRL
                     SAGPLPTSETVALRRVTFRLYAMLKVHLAEEHLYLGIIEHGVSAEAAEKLAAAMDHSG
                     IVAF"
BASE COUNT          602 a         1385 c         1329 g          632 t
ORIGIN      
        1 ctcttcccgg agggcggcct gtcgctcgac gagttccagt acgtcgccga gaccgacgag
       61 gacgccaacc cgggtcagac cagcatcccg ggcgtgtacg tcgccggcgc cgcatccggc
      121 gccaaggaca tcgccgattc gatcctgcac gccggctcga ccgtcgccca ggtggcggcc
      181 tggctggaga agatgaaggt gccggcatga ccgagcagga gcgacgaatc ggggtctatg
      241 tctgccattg cggcggcaac atctccgact acgtcgacgt cgaccaggtc gtcgccgccg
      301 cccagggcga gcccggcgtg gtcgtcgccc gccagatgat gttcgcctgc tccgactccg
      361 gccaggggga gatggagcgc gacatcgagg agcagcatct cgacggcctc gtcgtcgcct
      421 cgtgctcgcc caagcttcac gtcgtgacct tccggggcgt ggccaagcgg gccggactca
      481 acccctacga gtacacccag gtcaacgttc gcgaacaagc gtcgtggatc cattccgacg
      541 acgcccaggg cacgaccgac aaggccatcc acctggtcaa ggccgggatc gcccggacgc
      601 tgctgacgga gccactcgag ccgatcgtcg tcgagacgac ccagcggacg ctcgtcgtcg
      661 ggggcggtgt ggccgggctg cgggccgcga tcgggctcgc cgacatcggt cttggggtct
      721 tcctcgtcga gcgaaccgcg gtcctcggcg gctgggtcgg aacgttcggg gcgatgtacc
      781 cgaccggcaa ggacggcaag cagctgatcg ccgagctgct cgaagggatc cgccgccggc
      841 cctcgatccg cgtcttcacc gaagccgaga tggtcagcaa gagcggcagc ttcgggaact
      901 acgaggtcgg gatccaggtg ggcggatcga accccgagac gctgtcggtg gaggttggct
      961 cgatcgtcgt gacgacgggc gtcgacacct atgcccccga ggtcggcgag ttcggctacg
     1021 ggatcgacgg ggtcgtgacc ctgcccgagt tcaagacgct gatcgacgcg tcgggcggcc
     1081 cgctcgtccg aaacgggcgc ccggtccgca ccgtcgccta catctactgc gtcggcagcc
     1141 gtgagccggc gggcgagccg ggtgccaacg agtactgctc gcgctactgc tgcgcggcga
     1201 ccgtcgccgc gtccgtccag gtggcctcgc gcgacgcgac gatccaccag taccacgtct
     1261 atcgcgacat gcggacgtac ggcaagttcg agacgatgta cacggagtcc cgcaagctgg
     1321 gatccgtcta cctgaagttc gccgacgacg caccgccgac cgtgacccgg accgatgccg
     1381 gcggcctgct cgtgaccgtc aaggacgtcc tgaccggcgg cgaggagctg gccatcccgg
     1441 ccgatctcgt cgtcctcgtc accgggatga tcccgcgccg caacgaggag ctcatcggag
     1501 tcctcaagct gccggtcgac aaggacgggt tcttcaacga gatccatccg aagctgcggc
     1561 cggtcgagac ggtggtcgac ggggtgttca tcgccggcgc ctgccagggg ccgaagaact
     1621 cgagcgagag cgtggcctca gggctggcgg ccgtcaccca gagcgcggtc atcctcaaga
     1681 aggggttcgc cgagctcgac ccgctcgtgg cgatcgtcca tgccgacgcc tgcacggcct
     1741 gcaacctgtg cctcgaggcg tgcccctacg gcgcgatcgc gatgggcgcc ctcggtgatc
     1801 gctccgtggc cgtcatcagc gagaccggct gcaagggctg tggcgggtgc gtcccggtct
     1861 gccccgagaa cgccatcgac ctgctcggct acaccgacgg tcagatcacc gcgatgatcg
     1921 acaagatgct ggaggtctcg gtcgcatgaa cgccccgaat cgcgagcttc gcgaggtcat
     1981 ccgcgaggag ccgatcatgc gggcgcggat cctcgagctg ctcgatcccg aaccgcgcac
     2041 cgttcccgag ctggccgccg cgatggactg cccgacccac gaggtcctct tctggatcat
     2101 gggcatgcgc cgctacggcc gggtccgcga ggtcaagggc gtgaccgacg aggggtactt
     2161 ccgctacgaa gcgatcgcgc aggatccgtc atgaccgccc tcgtcgacac gaacctgctg
     2221 agcgacctgc agcgctacgg cgcggcggac gtctccgcct gcttcagctg cgggacctgc
     2281 acggcgacct gtccgctggt cagcaacgat tcgacgttcc cgcggcggat gatccgctac
     2341 gcccaggtcg ggctgcgcga ccggcttctt tcgagcaagg agctgtggac gtgctacgcc
     2401 tgcggcgagt gctcggagac gtgtcccacc caggccgagc cgagcgagtt catggccgcc
     2461 gcgcggcgct acgccatcgc cggctacgac cggaccggtc tcgcccggac gatgtatacc
     2521 cggcccgtga tcgggacggt catcgcggtc gtcctggcgg cgttcttcgc cctcttcatg
     2581 tacgcggccc acggccccca gagtgccggg tcgctggacg tcttccggtt catcccggaa
     2641 gagctcatcc acaacctcgg gatcgcgatc atggtcatcg tcttcgcgac cggcctcgtc
     2701 ggcgtcgtga cgatggcccg ggccgtcagc cgagaaggcg gcgtgagcct gcgatccgtg
     2761 gtcggtggcc gggcggcgct gcgtcgctcg gcgggcgcgc tgtggaccgc cctcgcggtc
     2821 gagacactcg gccagcgtcg ctaccgcgac gactgcgacg ccgatgctgc ggcccagcgc
     2881 tggtaccttc gtcgctggtt ccttcacgcg gccacgatgt ggggcttcct ggggctgctc
     2941 gcggcgacga tcctcgacta cgggctggcg atcgtcggga tcaaggcgac cggcacggcg
     3001 gtgccgatct ggtatcccgt ccgcctcctc ggcaccgtcg ccggcatcct cctggtctac
     3061 ggcgcgacga tgctcatcgt ccagcggtat cgcggcaagg agcgctcggt ccgccactcg
     3121 acgagcgcgg actggaccct cctggcgctc gtctggatca ccggggtgac cggcttcgcg
     3181 ctcgagctcg cgctctacct gccgagtgcc ccggcctggg gctactgggt cttcctgttc
     3241 cacgtcgcga tcgccatgga gctcgtgatc ctcgctccgt tcatgaagct ggctcacgcg
     3301 gtctaccggc cggtggccct gttcttcctc gccctggcgg gcacaccggc cgccaggatc
     3361 gacgaggggg gcgtgcatgg ctgaaacgtc gacattggcg acatcaccaa cctggatgga
     3421 ggtctgacat gaccgcgagc ttgccccaag tctcccacga gcaccacgac cggctgaccc
     3481 gacacctgga caccatgccg gcgctcggtg acctgatcgg taccgcgtcc gtcgccgagc
     3541 ttcggccgcg cgtcgacgag atcgccgact tcctcaccgg gctgcttctg ccgcacatgg
     3601 aggcggccga gcacacgctc tatcccgagc tggagcggat gctccagaac cgtcattcga
     3661 tgacgccgat gcggcgcgag cacgatgaga tccgggccct cgtcaccgaa ttgggccgat
     3721 tgcagcaccg cctgtcggcc gggcccctgc cgacaagcga gactgtcgca ctgcgtcggg
     3781 tgacgttccg cctctacgcg atgctcaagg tccacctcgc cgaggagcac ctgtacctcg
     3841 ggatcatcga gcatggcgta tcggctgagg ccgccgagaa gcttgccgcg gcgatggacc
     3901 acagcggcat cgtcgccttc tgatccggac cgtcacccgt cgcccgcc
//
LOCUS       DATAIW010000444         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_134674, whole genome shotgun sequence.
ACCESSION   DATAIW010000444 DATAIW010000000
VERSION     DATAIW010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134674"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..777
                     /locus_tag="VHM48_13250"
     CDS             118..777
                     /locus_tag="VHM48_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2756429.1"
                     /translation="MFVQQHPVEALALIAFFLLLAFPVHEFAHAYAAYRLGDSTAKLF
                     GKLTLNPIAHFDQVGGTMLVLSILLTGFPFGFAQTPVNPRNLRGKYGEVIVAGAGPVS
                     NLIMAAIVAIPLRILISQPDLILSNFALWDRIGTTLVFIVEYSILLGLFNLIPIPPLD
                     GGAIMLSLVSPRTAWQLRPMLAQYGFFLIILLIIPLGGQSILGRVLLPIVNAIYGVLV
                     G"
     gene            758..1249
                     /locus_tag="VHM48_13255"
     CDS             758..1249
                     /locus_tag="VHM48_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756430.1"
                     /translation="MASWWAEKVRQVGAHVRARVSDAERRQLVTWLTADQIALFDGMH
                     VADRRHGLDVVARLRADGATDPDLLVAGLLHDCAKGDTGVLPRIAFSLGQRYGTWIWG
                     LAGRVPGWRAVLERLRVHAEASARLAAAAGCSPATVELIGHQDAPIDPVAGEQLRLAD
                     EAS"
     gene            1249..2160
                     /locus_tag="VHM48_13260"
     CDS             1249..2160
                     /locus_tag="VHM48_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2756431.1"
                     /translation="MSIDVADLRPAAPAVMFGAGRRPEAATQVRMESFDGPLALLLTL
                     IEARQLDVMTVPLGALADAYLDALATLETDRLGNISAFVAVASQLILIKSRAILPRRV
                     VEAPGVLPDDEADPEAELRARLLLYRAHRDAGRRLQDEALSRVGLFRREPSVAAAAGQ
                     AGARPPDGPPLDVAVLAGALERLSRIAPPVAPPPETVARTITLTERAAIIRAAIRGAG
                     TIVLQDLLAGVRDRVVVAVTFLALLELMKRREIVVSQAEPWGPIVARATTAEERALGR
                     GAGPEALAAEAAAAADEPLDETLESFA"
BASE COUNT          296 a          869 c          784 g          358 t
ORIGIN      
        1 gcggggcgat cagcgccgag gagtacgagg cgaagaaggc cgacctgctg cgccgcctgt
       61 agcattccat ccagcggcga cggccgcccg ctcggcagca ccgcgaggga tcgactcttg
      121 ttcgttcagc aacacccggt cgaagccctc gcgctcatcg cgttcttcct cttgctggca
      181 ttcccggtcc acgagttcgc ccatgcctac gcggcgtacc gactcggtga ctcgacggcc
      241 aagctgttcg gcaagctgac gttgaatccg atcgcccact tcgaccaggt cggcgggacg
      301 atgctcgtcc tctcgatcct gttgaccggc tttccgttcg gattcgctca gacgcccgtc
      361 aacccgcgca acctgcgggg caagtacggc gaggtgatcg tcgccggggc tggaccggtc
      421 tccaacctga tcatggccgc aatcgtcgcg atccccctgc ggatcctgat ctcgcagccc
      481 gacctgatcc tgtcgaactt cgcgctctgg gatcgcatcg ggaccaccct tgtgttcatc
      541 gtcgagtaca gcatcctgct cggcctgttc aacctgatcc cgatcccgcc gctcgacggc
      601 ggcgcgatca tgctgtcgct cgtctcgccg cggaccgcgt ggcaactccg accgatgctc
      661 gcccaatacg ggttcttcct gatcatcctg ctgatcatcc cgctcggcgg ccagtcgatc
      721 ctgggccgcg tcctgctccc gatcgtcaat gcgatctatg gcgtcctggt gggctgagaa
      781 ggtccgccag gtcggcgccc acgtccgggc gcgggtgagc gatgccgagc gccggcagct
      841 cgtgacctgg ctgaccgccg atcagatcgc gctcttcgac gggatgcacg ttgccgaccg
      901 ccggcacggg ctcgatgtcg tcgcccgtct ccgggccgac ggggcgaccg atcccgacct
      961 cctggtcgcc gggctgctcc acgactgcgc caagggcgac accggcgtcc tgccccggat
     1021 cgcgttctcg ctggggcaac ggtacgggac gtggatctgg ggcctcgcgg ggcgagtccc
     1081 cggctggcgg gccgtcctgg agcgtctccg ggtccatgcc gaggcatccg cccggctggc
     1141 ggccgccgcc ggctgcagtc ccgcgacggt cgagctgatc ggccaccagg acgcgccgat
     1201 cgacccggtc gccggcgagc agctccgtct cgccgacgag gcctcgtgat gtcgatcgac
     1261 gtcgcggatc tccgtccggc cgcgcccgcg gtgatgttcg gtgccggtcg ccggccagag
     1321 gccgcgaccc aggtccggat ggagtcgttc gacggccccc tggcgctcct cctgaccctc
     1381 atcgaggccc gccagcttga cgtcatgacg gtcccgctcg gagccctggc cgacgcctat
     1441 ctcgacgcgc tcgccacgct cgagaccgac cggctcggga acatcagcgc gttcgtcgcg
     1501 gtcgccagcc agctgatcct gatcaagagt cgggcgatcc tgccgcgccg cgtcgtcgag
     1561 gcgccggggg tcctgccgga cgacgaggcc gatcccgagg ccgagctgcg ggcccggctc
     1621 ctcctgtacc gggcccatcg cgacgccggc cggcgcctcc aggacgaggc actcagccgg
     1681 gtcgggctgt tccggcggga accctcggtg gccgcagccg ccggccaggc gggtgcccgt
     1741 ccacccgacg gcccgccgct cgatgtcgcc gtgctggccg gggcgctcga gcgcctgtca
     1801 cggatcgcgc caccggtcgc gccgccgccc gagaccgtcg cccggacgat caccctgacc
     1861 gagcgggcag cgatcatccg ggccgccatc cgcggtgcgg gcaccatcgt cctccaggac
     1921 cttctcgccg gggtccgcga ccgggtcgtc gtggcggtca ccttcctcgc gctgctcgag
     1981 ctgatgaagc gccgcgagat cgtcgtcagc caggccgagc cgtggggccc catcgtcgcc
     2041 cgggcgacca cggccgagga acgggccctg ggcaggggcg ccggcccgga ggcactcgcg
     2101 gccgaggctg cggccgccgc cgacgagccg ctcgacgaga ccctggagtc gttcgcgtga
     2161 tcgacgagcg cgtggcggcc gacgcgcccg acggggtcga gtccgatgat tcgccgccgg
     2221 cacccgagat cccgcccgag ctgaccgagg cccagctgga ggcgctcctg ttcgtggccg
     2281 aacggccgct gacccggcgc gagatcg
//
LOCUS       DATAIW010000445        20413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_194541, whole genome shotgun sequence.
ACCESSION   DATAIW010000445 DATAIW010000000
VERSION     DATAIW010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 20413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20413
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194541"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VHM48_13265"
     CDS             complement(<1..546)
                     /locus_tag="VHM48_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756432.1"
                     /translation="MPERGGLSRTAPAARSRPSCRPGSSAGDRRSTRSGTGHRPAAVG
                     TGDLPLLRRLLGEPALTADLGGPETADQIERRHECYLATNDPATGLLFAIVLPPGTAV
                     ASVGYWERDEDGETAWETGWFVLPEYQGRGIATAATPLAVEAAWTAIARPVHAYPNLE
                     NVASNTIAQNAGLRLIGPWELE"
     gene            complement(464..1186)
                     /locus_tag="VHM48_13270"
     CDS             complement(464..1186)
                     /locus_tag="VHM48_13270"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX2756433.1"
                     /translation="MRARRTIVAMGGGGFQMEPDNPLLDDHVLALARERSGRARPRVC
                     LIPTATTEDPGVIADFERLFAPRAEPRVLRLFVREDDDLAAVIRDQDAVYVTGGNTAN
                     LLALWRLHGLDRVLRDAWNAGVVMAGMSAGAICWFESCTTDSFGPTLRPLAGGLGILH
                     GSLSPHYHGEAQRRPLYLRLVGDGTLPAGYAVDDSAALVFDGRELVEVVTSAPDAGAW
                     RVEPDGAGSAIETELPTRFLGG"
     gene            complement(1197..2183)
                     /gene="glpX"
                     /locus_tag="VHM48_13275"
     CDS             complement(1197..2183)
                     /gene="glpX"
                     /locus_tag="VHM48_13275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX2756434.1"
                     /translation="MEQLLALEMVRVTEAAALASARFMGRGEKDEADRAAVEAMRATM
                     DEIEFAGTIVIGEGERDEAPMLWIGEPVGRRTPAGVENNPEIDIAVDPLEGTNLVAHG
                     QPGAITVLAASEKGGLVHAPDTYLEKLCVGPVAAGKVDIRESPTENLRRIASALGRAV
                     RDITVVILERPRHQALIAEVRAAGARIQLISDGDLSAAISTAVSGTGVHAVMGIGGAP
                     EGVITAAALRCLGGEIQARFRYRSDEERARGARMGHGDEDRVYHTEDLAPGENLVFSA
                     TGVTAGDLLEGVRFFGGGARTHSLVMGYQTKQVRFVDTVHMFDRDRPPSVRL"
     gene            complement(2238..3785)
                     /gene="purH"
                     /locus_tag="VHM48_13280"
     CDS             complement(2238..3785)
                     /gene="purH"
                     /locus_tag="VHM48_13280"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX2756435.1"
                     /translation="MRALLSVANREGIVSLARELLALDVEVFATDGTRDHLAADGVEV
                     RSVSDLTGLPTLIGGQVKTFHPAIYAGILARRDIPSQMDELVEQGIGAIDIVVVNVSP
                     FAPAVGAHLVAIDEAIEMIDVGGAALLGAAARNGAGVAAVGSPEHYPKLVTELKRHHA
                     LSAELRAQLAADAFGVVAAYYAEIAAYLNQISNNTFPKRLAVVLEKVDDLRYGENPHQ
                     RAAFYRETTHRSGTLADATQIRGERPSFNNYLDLDTAYRIARDFTAPTVAIVKHTDPV
                     GIASGDELVEAYRRALETDAVAAFGGIVAVNRELDGATAREIAANSYEAVVAPAYSQA
                     ALGILKAKAGLEILAVPPDPTEGMRDYGIASLDFKRVAGGLLVETLDEVGLDRGRLQV
                     VTKRRPTLDELNDLLFAWRAVCHVRSNAIVLARSGATVGIGAAQASRQTSVEIALRKA
                     GDRSKTAVMASDAYFPFPDGIQIAAQAGVTAIVQPGGSIRDEMAIEVADRHHLAMVFT
                     GRRHFRH"
     gene            3960..4445
                     /locus_tag="VHM48_13285"
     CDS             3960..4445
                     /locus_tag="VHM48_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756436.1"
                     /translation="MRLLPWEYLFTRFSAHTFPDTYTITWVATIVLLVGLVIVYNART
                     RALHRHAPYLDMYEWLLWTGISLFGLILVAAVFNFYLIVMLVILFTGLAVLIWVRFIK
                     FPPMFEVYEQKLAKQRYFTRSKFAHPEATIRPKGAKATKAGGSRPNAQRARSSRSRRR
                     R"
     gene            4475..4951
                     /locus_tag="VHM48_13290"
     CDS             4475..4951
                     /locus_tag="VHM48_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2756437.1"
                     /translation="MPIEVRRFGVGHRRPDGPPGTTGVSGQVIHSDARGMISELAFAR
                     NGRIEPHSNPNTTWFIVIEGGGWVIVDGELLRVAAGEAVLWPPDVPHGAWADHGHMRA
                     FVVEFAGPDDAFFRGILEGRPVATIGPGAGVVGRGEGTLTGPDHPPPQDPEAGEPH"
     gene            complement(5208..5942)
                     /locus_tag="VHM48_13295"
     CDS             complement(5208..5942)
                     /locus_tag="VHM48_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX2756438.1"
                     /translation="MSKRRSARRRHGAPFVLALTLVGGVALAVPTGLAARVVRVVTGA
                     PVTVDLGGPLPACRADAIPAVHRDEADWAVTLLDPMFSLEPDDNPADLVPVAEAGIAG
                     GGDVRLLVVGDLRAMTRAARQDGVTLRVRSAFRSYDAQIRTVESLERAYGREWTERSA
                     ARPGHSEHQLGTALDLDGGDAWLAGHAWEHGFVVSYPADRSPRFTCYKPEPWHVRYAG
                     RAEAAAVHDSGLSLREWLWLHPDGIG"
     gene            complement(6120..7649)
                     /locus_tag="VHM48_13300"
     CDS             complement(6120..7649)
                     /locus_tag="VHM48_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX2756439.1"
                     /translation="MRKLLLSVSVAALLASACGSASTTGPANYGGATAPPDHHQPTYP
                     PQPAGPAPTPYGGVTYRDPGTNPWVDPARDDHSTFALDIDTASYTIAQRYVQDGHQPD
                     PASVRVEEWVNAFAQGYPFPEEDAFAIVADGGPTPFTAEDEVLLRIGLQARAVAARDR
                     QQASLTFVLDTSGSMEQGGRLEIVKDALRILIHGLGRNDRVAIVSFGSDAQVVLGPTP
                     ADQEGTILDALDRLHPGGSTNLEAGLRLGYELARKILTENGIDRIVLASDGVANVGLT
                     DPTSILEAISRDAAAGIQLVSVGVGMGNYNDTLLEQLADQGDGFYAYVNTNDEARKLF
                     SEDLTSTLQTVALDARAQVAFDRESVAAYRLVGYENRAIADRDFTNDGVAAGAIGAGH
                     AVTALYAIRLEPSAGPRSHLARVTVRWTDPDRGGAVDLGRDVALSDLARSFGATNPQF
                     RFDAIVAQTAEVLRGSEFAGRLDLRDVADVADREAEALPATEEVHAFLDMLRDLGRLR
                     D"
     gene            complement(7816..8451)
                     /locus_tag="VHM48_13305"
     CDS             complement(7816..8451)
                     /locus_tag="VHM48_13305"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2756440.1"
                     /translation="MRFDDAVERLAVLPSPLPIGRAELMPVFTDGRLRPRLDPTATAR
                     PAAVLALIVPGPAGAAHIVLTERPSYDGHHSGEVSFPGGKAEPGDTDDAATALREAAE
                     EIGLDPIAAGVRVVGRLDEVFIPVSDFRITPIVAVAERPPVLHPNPDEVARILTPPIE
                     AFLPHAEVEIVERTIRDWPIRYGGYRIDGLHVWGATARILGQLGAILGPRA"
     gene            complement(8534..11323)
                     /gene="clpB"
                     /locus_tag="VHM48_13310"
     CDS             complement(8534..11323)
                     /gene="clpB"
                     /locus_tag="VHM48_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX2756441.1"
                     /translation="MQLDRYTQKAQEVIAAAQRAAESMQSPVLDAEHLLHALVEPDDG
                     VPAETIRRLGVDLPAFRDELAGILGKRARIHGGSLSLDPRAKRVLELGEQEARRLGDE
                     YVSTEHLLLGVLEIGGEGRQLLERHGAGREAVLGALQSVRGGQRVTSPNPEGTYAALE
                     KYGRDLTAEARAGRLDPVIGRDEEVRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIIRGDVPEGLKDKRVISLDLGALIAGAKFRGEFEERLKAVLKEIKDSDGQVILFID
                     ELHTVVGAGAAEGAMDASNLLKPMLARGELHTIGATTLDEYRKHIEKDAALERRFQPV
                     FVDEPTVEETISILRGLRERYEVHHGVRITDSALVAAATLSQRYITERFLPDKAIDLV
                     DESASRLRMEIDSMPVELDEAERRRMQLEIEREALRKETDDASKARLATLERELADLE
                     ESTGALKQRWEAEKTAIAAIRASKTELEQLQVRIEQAQRETDYETAAKLQYETLPALQ
                     KRLAAETDTLAALQGPGSLLREEVNADNIAEIVAAWTGIPVTRLMEGELEKLVKMEER
                     LHLRVVGQDEAIEAVSDAVRRARAGLGDPRRPLGSFLFLGPTGVGKTELARALAEFLF
                     DDDGAMTRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYQVILLDEIEK
                     AHPDVFNILLQVLDDGRLTDSQGRTVDFKNAVIILTSNVGSQLIGAVGGGGRGQDDGA
                     YEAMKRQVTDALRAQFRPEFLNRIDEIIVFHALSEADLEQIVELLLRGLDRRLAGQDL
                     RIELTAAARGLIVREGTDPSFGARPLKRTIQRLVENPLARALLKGEFAPGDTIAVDAD
                     AVTGTLVFSTERSTVVAEAANRRDARTPGGRTEADGDEAVGATSGGGRRPKRGLDLPE
                     IDNPKPERDGGELVN"
     gene            complement(11469..12434)
                     /locus_tag="VHM48_13315"
                     /pseudo
     CDS             complement(11469..12434)
                     /locus_tag="VHM48_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            complement(12504..15023)
                     /gene="lon"
                     /locus_tag="VHM48_13320"
     CDS             complement(12504..15023)
                     /gene="lon"
                     /locus_tag="VHM48_13320"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX2756442.1"
                     /translation="MAKRPTQDRDDQPTTVNITTPSGPRELPLVALRETVIFPEMIVP
                     LQVGREKSVNALNAAVAEGGPIALVTQRQPEQEDIDSPSELYTVGTLAKIAQVVQLQD
                     GTVRAIVQGQTRLKLINFVANEPYLRATVEELHDASGASLEVQALMRTVQAQIEQYVA
                     NGAPVPPEAAVAARNISEPGLLADMVAYSPDMSTEQRQELLETVDVKERLKLVSNFLA
                     RQIEILELKGRIQSEVKSEMDKTQREYILREQLKAIQRELGEDDPQQAEINELRDKVE
                     AAGMPEEIKTRAIKEVDRMSRIPSASPEVGVIRTYVDWLVGLPWNVATDDQLDIKQAA
                     KILDEDHYGLEKIKERILEYLAVRTLADQIRSPILAFVGPPGVGKTSLGKSIARAMGR
                     KFVRMSLGGIHDEAEIRGHRRTYIGALPGRIIQNIKTAGSNNPVFMLDEVDKIGMDFR
                     GDPSSALLEVLDPEQNNTFQDNYLEVPFDLSKVLFVATANLLDPIPAALRDRMEIVYL
                     PGYTEQEKIEIGKRFLIPKQMKNHGLSGKNIVIPDGTMIELVRSYTHEAGVRNLEREL
                     ANVMRKVARTVAEGRKRKTIIDLKRLAEYLGPPRYEYGELEAEDQIGSATGLVVTEVG
                     GDVIAIEVTKMEGKEDFILTGQLGDVMRESARAGLSWTRAHATELGISREIFEKNTLH
                     IHVPAGGTPKDGPSAGITMALAIVSALTGIPVRKDVAMTGEITLRGRVLPIGGLKSKI
                     LAAHLAGAKMVILPRKNEKDLRDIPDEIRKSMKLVLVDSMEQVLETALRRKPKALVIA
                     QPPTVVKPGDAPSDAPEREERVRRTSFPPTDQPPVVVDRHR"
     gene            complement(15100..15483)
                     /locus_tag="VHM48_13325"
     CDS             complement(15100..15483)
                     /locus_tag="VHM48_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2756443.1"
                     /translation="MSQGRVEKDPRRPVYVISVAATLVAAHPRTLRIYEDEGLICPAR
                     TPSNIRLYSDEDIRRLTWIRHLTQDRGVNLAGIRLLFELEERLGTRILEALYAEGTRR
                     QAADRAAAEASPKPDRAGTVPDDRS"
     gene            complement(15488..16021)
                     /locus_tag="VHM48_13330"
     CDS             complement(15488..16021)
                     /locus_tag="VHM48_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2756444.1"
                     /translation="MTIVRRPSPFGELLSLRSAMDRLFDDSFVRRPFGAGFDAPTQLP
                     LDVTRTADALVIEAALPGIRPEDVEITVEDRTLSIRGEDRDVQQAGEGEALISEIRRG
                     TVGRAIILPTGLEPDKATATFENGVLTLRIPKAESVKPRQIRISPTVNGASAEVRAAE
                     TTGNGKTPVAAGTGQGA"
     gene            complement(16301..16981)
                     /locus_tag="VHM48_13335"
     CDS             complement(16301..16981)
                     /locus_tag="VHM48_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2756445.1"
                     /translation="MFVLVIGGGKVGYYLTKELIGSGHEVVLMEKEQNRANQIADEIG
                     SVVIAHDGCEGKYLGQAGANRADIVAAVTGDDEDNLVICQMAKHHFDVPRTIARVNNP
                     KNETLFKHLGVDELISPTRMILGSIEQDIPVHELLHLAALGEGELELIEAHLQAGSPA
                     IGKTPRDLSMPDGCSLFAVIRDGVATPLRPETVLAEGDKVIAIGKAESGPQLHEQLIG
                     DQEAAPAG"
     gene            complement(16987..17544)
                     /locus_tag="VHM48_13340"
     CDS             complement(16987..17544)
                     /locus_tag="VHM48_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2756446.1"
                     /translation="MKTVIIGCGRVGSVLADALDRAGHQVIILDPNTTAFDRLPGTFK
                     GAAIRGDGTDEDTLRRAGAESADLFMAMTEGDNRNIMSAQLATEALDAKQVIAKINDP
                     LRASAYAELGVATLCRTNLMASAVSDFVGLGLTFGPGLSAATGHHPGGEHHGPLDESL
                     PAATAFAAPTDPTPAPVAPISAREG"
     gene            complement(17541..18005)
                     /locus_tag="VHM48_13345"
     CDS             complement(17541..18005)
                     /locus_tag="VHM48_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2756447.1"
                     /translation="MPPESQAHPSFRRAVVALSGHPNGQRLVRLVAQLAKAHKAELIG
                     VHVVEMDWSVPLDMDVAGRSEEIQRVLDLAEGTAESSGVRLEPVLLQARDVGAALVDE
                     AVEREADLLVIGLAYRTKFGGDFEIGRTIPYVLKNAPCAVWVVREPIPEETS"
     gene            complement(18073..20130)
                     /locus_tag="VHM48_13350"
     CDS             complement(18073..20130)
                     /locus_tag="VHM48_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2756448.1"
                     /translation="MIGGRRPLQGRKPADRRVRVDRPHAPYFRYTGPGQMVAKAAAHT
                     PKTAVGRTWARIRKVAIGRPLASEEEAGERLTKKKALAIFSSDAISSSAYATQEIIRV
                     LAVAGATALAFSVGVSIAIAVLLAVVATSYRQVCRAYPGGGGAYAVAKENLNPLLGLV
                     AAAALLIDYVMTVAVSTASAIGQIVSVVPDLDGVKIEIAITVIVLMTVANLRGLRESG
                     NIFAVPTYAFVGLALLMVGLGIVNIVTGNAHPIVTPNAEVVSGGELSIFLLLKAFAGG
                     SVALTGVEAIANGVPAFKPPEAKNAANTMTAMAILLGILFIGITIISHAYQILPTVGD
                     IPTTVSLVAGNVFGNGSLLFVLFQAATALILFLAANTSFNAFPRLGAILAVDGYMPRQ
                     FSYRGDRLAYSWGIVVLSTVAAFLIWLFEGNVTALIPLYSVGVFVCFTLSQIGMVKHW
                     RSEHGSGWQWRLGINAFGAVLTAVVLVVVVSVKFVDGAYLVVILIPTLVAMMLFIHRQ
                     YDASRRELSVRPDKVFTSLHREERAVVPIPGVNRAVIHAVNVARSIADDVRAVFITED
                     AEVGLAVREQWERQVPGVPLVVVESPYRALTGPLGAYLDVLDSAWPPDKEAPITFVVL
                     PEYVARRWWERILYNQSVKRLRSSLLGRPHTVVVNVPYRRDDPTMVERGHPSAGDEQS
                     AKP"
BASE COUNT         2886 a         6905 c         7352 g         3270 t
ORIGIN      
        1 ctccaactcc cacggtccga tcagacggag cccggcgttc tgggcgatcg tgttcgaggc
       61 gacgttctcg aggttcggat aggcgtggac cggccgggcg atagcggtcc atgccgcctc
      121 gacggccagg ggcgtcgccg ccgtcgcgat cccgcgcccc tggtactccg gaaggacgaa
      181 ccagccggtc tcccaggccg tctcgccgtc ttcatcgcgc tcccagtagc cgaccgatgc
      241 gaccgcggtt ccgggcggca ggacgatggc gaacaacagg cccgtggccg ggtcgttggt
      301 cgcgaggtag cactcgtgac ggcgctcgat ctggtccgcc gtctcaggac cgccgaggtc
      361 ggcggtcaac gccggttcgc cgagcagccg ccggaggagc ggtaggtcgc cggtcccgac
      421 ggccgcaggg cgatgaccgg tgccggatcg ggttgaccgc cggtcacccg ccgaggaacc
      481 gggtcggcaa ctcggtctcg atcgcgctgc cggcgccgtc cggctcaacc cgccacgctc
      541 cggcatcggg cgccgacgtg acgacctcga cgagctcgcg cccgtcgaag acgagcgccg
      601 cgctgtcgtc gactgcgtag cccgccggca gcgtgccgtc gccgacgagc cgcaggtaga
      661 gcggccgtcg ctgcgcttca ccgtggtagt gcggtgacag gctgccgtgg aggatcccca
      721 gcccgccggc caggggccgg agcgttggcc cgaacgaatc cgtggtgcac gactcgaacc
      781 agcagatcgc cccggccgac atcccggcca tgacgacccc ggcgttccag gcgtcgcgga
      841 ggacgcgatc caggccgtgc agcctccaca gcgccaggag gttcgcggtg ttgccgcccg
      901 tcacgtagac cgcgtcctgg tcccggatca ctgccgcgag gtcgtcgtcc tcgcggacga
      961 agaggcgaag aacccgcggc tctgcccgcg gcgcgaacag gcgctcgaag tcggcgatga
     1021 cccccggatc ctcggtcgtc gccgtcggga tcaggcagac ccgcggccgg gcgcgaccgg
     1081 atcgctcgcg ggcgagggcg aggacatgat cgtcgaggag tggattgtcg ggctccatct
     1141 ggaacccacc accgcccatc gcgacgatcg tccgcctggc gcgcaccgcc cgtcgtctag
     1201 agccggaccg acggcggtcg atcccggtcg aacatgtgga ccgtatcgac gaaccggacc
     1261 tgcttggtct ggtagcccat gaccaacgag tgggttcgcg ctccgccgcc gaagaagcga
     1321 acgccctcga gcaggtcgcc ggcggtgaca ccggtcgccg agaagacgag gttctcgccc
     1381 ggcgccaggt cctcggtgtg gtagacacga tcctcgtcgc catggcccat ccgtgcgccg
     1441 cgggcccgct cctcgtcgct tcgatagcgg aagcgcgcct ggatctcgcc gccgaggcag
     1501 cgcagtgccg cagcggtgat cacgccctcc ggcgcgccgc cgatccccat caccgcatgg
     1561 acgccggtgc cggaaacggc ggtcgagatc gcggccgaca ggtcgccgtc gctgatcagc
     1621 tggatccgcg cgcccgcggc acggacctcg gcgatcaacg cctggtggcg cggacgttcg
     1681 aggatcacga cggtgatgtc gcggacggcc cggccgaggg cggaggcgat ccgccggagg
     1741 ttctcggtcg ggctctcgcg gatgtcgacc ttgccggccg cgaccggacc gacacagagc
     1801 ttctcgaggt acgtgtcggg ggcgtggacc aggccgccct tctccgatgc ggccaggacg
     1861 gtgatcgcgc cgggctggcc gtgggcgacg aggttcgtcc cctcgagcgg atcgacggcg
     1921 atgtcgatct cgggattgtt ctcgacgccg gccggggtcc gtcgcccgac gggctcgccg
     1981 atccagagca tcggcgcctc gtcgcgctca ccctcgccga tgacgatcgt gccggcaaac
     2041 tcgatctcgt ccatcgtcgc ccgcatggcc tcgacggcgg cccggtccgc ctcgtccttc
     2101 tcgccgcgac ccatgaaccg ggccgacgcg agcgcggccg cctccgtaac gcggaccatc
     2161 tcgagcgcaa ggagctgttc catcgaaatc tccgcgggct gtcgggaggg attggtgcgt
     2221 tgcggaccgg cggtggttca gtgccggaag tggcgtctcc cggtgaagac catcgccaga
     2281 tgatgccggt cggcgacctc gatggccatc tcgtcgcgga tcgagccgcc tggctggacg
     2341 atcgcggtca cgccggcctg ggccgcgatc tggatcccgt ccgggaaggg gaagtaggcg
     2401 tcgctggcca tcaccgcggt cttcgagcga tcgccggcct tgcgcagcgc gatctcgacc
     2461 gacgtctggc gactggcctg ggcggccccg atcccgaccg tcgccccgct ccgggcgagg
     2521 acgatcgcgt tcgaccggac gtggcagacc gcgcgccagg caaagagcag gtcgttgagc
     2581 tcgtcgagcg tcgggcggcg cttcgtcacg acctggagcc ggccgcgatc gagaccgacc
     2641 tcgtcgaggg tctcgacgag cagcccgccg gcaacccgct tgaagtcgag gctggcgatc
     2701 ccgtagtcgc gcatcccctc ggtcgggtcg ggcggaacgg ccaggatctc gaggccggcc
     2761 ttggccttga ggatgccgag ggccgcctgg ctgtaggccg gggcgacgac cgcctcgtac
     2821 gagttcgcgg cgatctcgcg ggcggtggcg ccatcgagct cgcggttgac cgcgacgatc
     2881 ccgccgaagg cggcgaccgc gtcggtttcg agcgctcgcc gataggcctc gacgagctcg
     2941 tcgcccgacg cgatcccgac cgggtcggtg tgcttgacga tcgcgaccgt cggggcggtg
     3001 aagtcgcggg cgatccggta cgccgtgtcg aggtcgaggt agttgttgaa cgacggccgc
     3061 tcgccacgga tctgggtcgc gtcggcgaga gttccgctgc ggtgcgtcgt ctcgcggtag
     3121 aacgcggccc gttggtgggg gttctccccg tagcgcagat cgtcaacctt ctcgagcacg
     3181 acggcgagtc gcttgggaaa cgtgttgttc gagatctggt tgaggtaggc ggcgatctcg
     3241 gcgtagtagg cggccaccac cccgaaggcg tcggcggcca gctgggcacg cagctcggcg
     3301 ctgagcgcgt ggtggcgctt gagctcggtc acgagcttcg ggtagtgctc cgggctgccg
     3361 accgccgcga cgccggcgcc gttgcgagcg gccgctccga ggagtgctgc gccgccgaca
     3421 tcgatcatct cgattgcctc gtcgatggcg accaggtggg cgccgaccgc gggggcgaac
     3481 gggctgacat tgacgacgac gatgtcgatc gccccgatgc cctgctcgac gagttcgtcc
     3541 atctgcgacg ggatgtcgcg acgggcgagg atgcccgcgt agatcgcggg gtggaaggtc
     3601 ttgacctggc cgccgatcag ggtcggcagg ccggtcaggt cggagaccga gcggacctcg
     3661 acgccgtcgg ccgcgagatg gtcgcgggtc ccgtcggtcg cgaagacctc gacgtccagt
     3721 gcgagcaact cgcgggcaag ggagacgatc ccctcgcggt tggcgaccga cagcagggct
     3781 cgcacccgga cttctcctcc tccgatgcgg gaccgtgcta cgacgcgccg cacacgctcg
     3841 actcgctgcc gagtatagcg cgggccatgg catactccgt cgtcagcggc gaccggccgc
     3901 taccccggcc aggttcacga acccgggccg gcgaaccccg gacttgacga ggttggaacg
     3961 tgcggctgct tccctgggag tacctcttca cccggttcag cgcgcacacg ttcccggaca
     4021 cctacacgat cacgtgggtc gccacgatcg tcctgctggt cgggctcgtg atcgtctata
     4081 acgcccggac gcgagccctc catcgccacg ctccgtatct cgacatgtac gagtggctgc
     4141 tctggacggg gatcagcctg ttcggcctga tcctcgtcgc ggcggtgttc aacttctacc
     4201 tgatcgtcat gctcgtgatc ctgttcaccg gtctcgcggt gctgatctgg gtccgcttca
     4261 tcaagttccc gccgatgttc gaggtctacg agcagaagct cgccaagcag cgctacttca
     4321 cccgctcgaa gttcgcccac ccggaggcga cgatccggcc caagggcgcc aaggccacca
     4381 aggcgggcgg cagccggccc aacgcccagc gcgcccggtc gagccgcagc cggcgccggc
     4441 gctaggaccg gccgagcgcg agcccgagcg agcgatgccg atcgaggtcc gccgcttcgg
     4501 ggtcggccat cgccgaccgg acggcccgcc cggaacgacc ggcgtcagcg gccaggtcat
     4561 ccacagcgac gcccgcggca tgatcagcga gctcgcgttc gcccgcaacg gtcggatcga
     4621 gccgcattcc aacccgaaca ccacctggtt catcgtgatc gagggtggcg gttgggtgat
     4681 cgtcgacggc gaattgctgc gggtcgcggc cggcgaggcc gtgctctggc cgccggacgt
     4741 cccccacgga gcatgggcgg atcacggcca catgcgggcc ttcgtcgtcg agtttgccgg
     4801 gccggacgac gccttcttcc ggggaatcct cgaagggcgt ccggtcgcga cgatcgggcc
     4861 gggggcaggg gtcgtcggcc ggggagaggg gacgctgacc gggcctgacc acccgccgcc
     4921 gcaggatccg gaggccggcg agccgcactg agcgcgccac ccatctgctc cgcctgattc
     4981 cggcccacgt caggcgctgg gttccgtcat ccaccatgcc ccgtggtgca agtccgtggg
     5041 cggaatcagg aatcgggcgg acatgccacc gatcgagcgc aacctgcggt catccaccat
     5101 cgccgacggt ggatggcgtg accgaaccgt ccgatcggtg atcctcacca cgggcgatgg
     5161 tcggtggcag gaacgacccg aggtggacaa tcgggcccaa cttcggctca gccgatcccg
     5221 tcggggtgga gccagagcca ttcgcgaagg ctcaacccgc tgtcgtggac ggccgcggcc
     5281 tcggcccggc cggcataacg gacatgccac ggctccggct tgtagcaggt gaaacggggg
     5341 ctgcggtcgg ctggatagct gacgacgaac ccgtgctccc aagcgtggcc cgccagccag
     5401 gcatcgccgc cgtcgaggtc gagcgccgtc ccgagctggt gctccgagtg gcccggtcgg
     5461 gccgccgagc gctcggtcca ttcgcgaccg tacgctcgtt cgaggctttc tacggtccgg
     5521 atctgggcgt cgtagctgcg gaaggccgat cggacccgga gggtcacgcc gtcctggcgc
     5581 gcagcccggg tcatcgcccg gagatcgccg acgacgagga gccgaacgtc cccgccgccg
     5641 gcgatccccg cttcggcgac cgggacgaga tcggccgggt tgtcgtccgg ctcgaggctg
     5701 aacatcggat cgaggagggt gaccgcccag tcagcctcgt cgcgatggac ggccgggatc
     5761 gcgtcggcgc ggcaggccgg gagcggtcca ccgaggtcca ccgtgaccgg cgcccccgtc
     5821 acgacccgaa cgacccgcgc cgcgagaccg gtcggcacgg ccagggcgac cccgccaacg
     5881 agggtgagcg ccaggacgaa gggcgctccg tggcggcgac gtgcggatcg ccgtttcgac
     5941 atgagccgat catggcccgg gcggttgccg gttccgagcc ggggccttcc ggcggtggct
     6001 tgcgggatat cccgcgaccg tccgcagccg agaccatccc gacggacaag gtggtcggcc
     6061 ggtgccgctc ctccacggac accggccgac gcgggaaagg cggggtacga ggtcgcgggt
     6121 cagtcgcgga gccgaccgag atcccggagc atgtcgagga acgcgtggac ctcctcggtg
     6181 gccggcagcg cttcggcctc gcgatccgcg acgtcggcga cgtcccgaag gtcgagccgt
     6241 ccggcgaatt cgctcccgcg gaggacctcg gcggtctggg ccacgatggc gtcgaaccgg
     6301 aactgcggat tggttgcccc gaacgatcgg gcgaggtcgc tcagcgcgac gtcccgaccg
     6361 aggtcgaccg caccgccccg gtccgggtcc gtccagcgca cggtcactcg ggccaggtgg
     6421 ctccgcggtc cggcgctcgg ttcgagtcgg atggcataca gggcggtgac ggcgtgaccg
     6481 gccccgatcg caccggccgc gacgccgtcg ttcgtgaagt cccggtcggc gatcgcccgg
     6541 ttctcgtacc cgacgaggcg gtaggccgcg acgctctcgc gatcgaacgc aacctgcgcc
     6601 ctggcgtcga gggcgaccgt ctggagggtg ctcgtcaggt cctcggagaa gagcttccgg
     6661 gcctcgtcgt tggtgttgac ataggcgtag aagccatcgc cctgatcggc cagctgctcg
     6721 agcagggtgt cgttgtagtt gcccatgccg acaccgacgg agacgagctg gatcccggcc
     6781 gcggcatccc ggctgatggc ctcgaggatc gaggtcgggt cggtcagccc gacattcgcg
     6841 acgccgtcgg aggcgagcac gatccggtcg atgccgttct cggtcaggat cttgcgggcg
     6901 agctcgtagc ccagccggag gccggcctcg aggttggtcg agccacccgg atgaagccgg
     6961 tcgagcgcgt cgaggatcgt gccctcctgg tcggccggcg tcggtccgag gacgacctgg
     7021 gcgtcagagc cgaagctgac gatggcgacg cgatcgttcc ggccgagccc gtggatcagg
     7081 atccgcagcg cgtccttgac gatctcgagc cggccgccct gctccatcga cccggacgtg
     7141 tcgaggacga aggtcagcga agcctgctgt cggtcgcggg cggcgacggc gcgcgcctgg
     7201 agcccgatcc ggaggaggac ctcgtcctcc gcggtgaacg gcgtcggtcc gccgtcggcg
     7261 acgatcgcga aggcgtcctc ctcggggaag ggatagccct gggcgaatgc gttgacccat
     7321 tcctcgaccc ggacgctggc cggatccggc tgatggccat cctggacgta gcgctgggcg
     7381 atcgtgtacg aggcggtgtc gatgtcgagg gcgaacgttg agtggtcgtc cctggccgga
     7441 tcgacccatg ggttcgtgcc ggggtcccgg tacgtcaccc cgccgtacgg cgtgggggcg
     7501 ggacctgccg gctgaggcgg atacgtcggt tggtggtggt cgggtggggc cgtcgcacct
     7561 ccgtagttgg ccggccccgt ggtggaggca gagccgcagg cactcgcgag gagagcggcg
     7621 acagacacgg acaggagcaa cttgcgcatc gcgcaacctc cctggggaac gcgggtgagc
     7681 acgctcccgg tcctcgaggc ggggccggcg gccccgcagg tcccggatcg gcaccaccaa
     7741 ggtgtgccgt cgggccgtgc tgcagacgcc gcggcggttg cccgggttgc gtgaagattc
     7801 cttaagcccg agatctcaag cgcgaggtcc gaggatcgcg ccgagctggc cgaggatccg
     7861 ggcggtggct ccccagacgt ggaggccgtc gatccggtag cccccatagc ggatcggcca
     7921 gtcccggatc gtccgctcga cgatctccac ctccgcgtgg ggcaggaagg cctcgatcgg
     7981 tggggtgagg atccgggcaa cctcgtcggg gttcgggtgg aggaccggcg gccgctcggc
     8041 aacggccacg atcggggtga tccggaagtc gctgaccggg atgaacacct cgtcgagccg
     8101 accgacgacc cggacgccgg cggcgatcgg atccagcccg atctcctcgg cggcctcgcg
     8161 caacgcggtc gccgcgtcgt ccgtgtcgcc gggctcggcc ttgccgcccg gaaaactgac
     8221 ctcgccgctg tgatggccgt cgtagctggg ccgctcggtg aggacgatgt gggccgcgcc
     8281 tgccggcccc ggcacgatga gggccagcac ggcagccgga cgcgcggtcg ccgtcgggtc
     8341 gagccgcggc cggaggcgcc catcggtgaa caccggcatc aactcagccc ggccgatcgg
     8401 gagcggcgag ggcaggaccg cgagccgctc gacggcgtcg tcgaacctca tcgtgccgag
     8461 gattccccga agcaagcctc gatcagacgg tcgagatcgg cgccttctac cggaatctcc
     8521 tgccagctca gcatcagttg acgagctcgc cgccgtcgcg ttcgggcttc ggattgtcga
     8581 tctccggcag gtcgaggcct cgcttcggac ggcgcccccc gccgctcgtc gctccgaccg
     8641 cctcgtcgcc gtctgcctcg gtgcgcccgc ccggcgtccg ggcgtcgcgg cggttggcgg
     8701 cctcggcgac gaccgtcgag cgctcggtcg agaagacgag cgtcccggtc accgcgtcgg
     8761 cgtcgaccgc gatcgtatcg cccggcgcga actcgccctt gagaagcgcc cgtgcgagcg
     8821 ggttctcgac gagccgctgg atcgtccgct tcagtggccg cgccccgaag gacgggtccg
     8881 tgccctcgcg cacgatcaag ccgcgcgcgg ccgcggtcag ctcgatccgc aggtcctggc
     8941 cggcgagccg ccggtcgagg ccgcgcagca ggagctcgac gatctgctcg agatccgcct
     9001 ccgacagggc gtggaacacg atgatctcgt caatcctgtt gaggaactcg ggacggaact
     9061 gcgctcgcag ggcatcggtg acctggcgct tcatcgcctc gtaggcaccg tcgtcctgac
     9121 cgcgcccgcc gccaccgacc gcgccgatca gctggctgcc gacgttgctc gtcaggatga
     9181 tcaccgcgtt cttgaagtcg accgtccgcc cctggctgtc ggtcaggcgc ccgtcgtcga
     9241 ggacctggag gaggatgttg aagacatccg ggtgggcctt ctcgatctcg tcgagcagga
     9301 tcacctggta tggccggcgg cggacggcct cggtcagctg gccgccctcc tcgtagccga
     9361 cgtagccggg tggcgcgccg atcaggcgcg acacggagaa cttctccatg tactcgctca
     9421 tgtcgatccg ggtcatcgcc ccgtcgtcgt cgaagaggaa ctcggcgagg gctcgggcga
     9481 gctcggtctt gccgacgccc gtcgggccga ggaacaggaa cgagccgagc ggccggcgcg
     9541 ggtccccgag cccggcgcgt gcccgccgca cggcatccga cacggcctcg atcgcctcgt
     9601 cctggcccac gacccgaaga tggaggcgtt cctccatctt cacgagcttt tcgagctcgc
     9661 cctccatcag ccgcgtcacg gggatcccgg tccaggccgc gacgatctcg gcgatgttgt
     9721 cggcgttgac ctcctcgcgc aggagcgagc ccggaccctg cagcgccgcc agggtgtcgg
     9781 tctccgccgc cagccgtttc tggagcgccg gcagggtctc gtactggagc ttggccgcgg
     9841 tctcgtagtc ggtctcgcgc tgggcctgct cgatccggac ctggagttgc tcgagctcgg
     9901 tcttggacgc gcggattgcc gcgatcgcgg tcttctcggc ctcccagcgc tgcttgaggg
     9961 cgccggtcga ttcctcgaga tcggcgagct cccgctcgag cgtcgccagc cgcgccttcg
    10021 atgcgtcgtc ggtctccttg cggagcgcct cgcgctcgat ctcgagctgc atccgccgcc
    10081 gttcggcctc gtcgagctcg accggcatcg agtcgatctc catccgcagc cgcgacgccg
    10141 actcgtcgac gaggtcgatc gccttgtccg gcaggaatcg ctcggtgatg tagcgctggc
    10201 tgagcgtcgc cgccgcgacg agggcggagt ccgtgatccg gaccccgtgg tggacttcat
    10261 agcgctcgcg caggccgcgc aggatcgaga tcgtctcctc gacggtcggc tcgtcgacga
    10321 agaccggctg gaagcggcgc tcgagggcgg cgtccttctc gatgtgcttg cggtactcgt
    10381 cgagcgtcgt cgccccgatc gtgtggagct cgccccgggc gagcatcggc ttgaggaggt
    10441 tggaggcgtc catcgccccc tcggcggcgc cggcgccgac gaccgtgtgc agctcgtcga
    10501 tgaacaggat cacctggccg tcgctgtcct tgatctcctt gaggacggcc ttgaggcgct
    10561 cctcgaactc accgcggaac ttcgctccgg cgatcagcgc gccgaggtcg agcgagatga
    10621 cccgcttgtc cttgagccct tcggggacgt cgccgcggat gatccgctgg gccaggccct
    10681 cgacgatcgc cgtcttgccg acgccgggct cgccgatgag gaccgggttg ttcttcgtcc
    10741 ggcggctcag gacctggacg acgcgccgga cctcctcgtc gcggccgatg accggatcca
    10801 gccggccggc gcgggcctcg gcggtcaggt cccggccgta cttctcgagg gcggcatagg
    10861 ttccttccgg gttcggcgac gtgacgcgct ggccgccgcg aacgctctgc agggcaccga
    10921 ggaccgcctc gcgaccggcg ccgtggcgct cgagcagctg gcggccctcg ccgccgatct
    10981 ccaggacgcc gaggaggagg tgctcggtcg agacgtactc gtcgcccagc cggcgggcct
    11041 cctgctcgcc gagctcgagg acgcgcttgg cccgcgggtc gagggacagc gatccgccgt
    11101 ggatccgggc gcgcttgccg aggattccag ccagctcatc gcggaaggcg gggaggtcga
    11161 caccgagccg gcgaatggtc tcggcgggga caccgtcatc gggctcgacc agggcgtgga
    11221 ggagatgctc ggcgtcgagg accggcgact gcatgctctc ggcggcacgc tgggcggcgg
    11281 cgatgacctc ttgcgctttc tgcgtatagc gatcgagttg catcgggggt tctccgggat
    11341 cgggcagatc tggttcgcga gtggtgggtc ggtcaggtgg tcgcccttgg atcgggctgg
    11401 tcgagcttgt cgaagaacgt ccgggcggct tcggccgtat cgtcgtcgag cttgggcggg
    11461 aggacgaccc gggcctttgc gtagaggtcg ccgttgcctt cgccgcggag ctggggcatg
    11521 ccctggccct tgagccggaa gcgatggccg ttctgggttc cgggcgggat gcgcaggagg
    11581 accatgccct tgagcgtcgt gaccggcacc tcgccgccga ggagcgcctc ggcgagggtg
    11641 atcggcagct cgcgctcgag gtcggcgccc ttgcgggcaa agaccgggtg gggccggacg
    11701 cgggtcgtga ccaggaggtc gccgccctgt gggccctggc cggacagctt gatcttcgtg
    11761 cccgtgtcgg cgccgcgtgg gatcgagacg tcgagccgac gtccgttgat ctcgacccgg
    11821 cgggtggtcc cgtggtaggc ctcctccagg ccgatctcgg cgatcgcctc ggccgtcgcc
    11881 cgcccagctc gactcggctg cgattgaccg ccggcgggct ggccgctcga cccgccggtg
    11941 gcgctgttga tgcccatgtc cgacaggatg tcgtcgaacg acggtccagc cccgccgcgt
    12001 cgcacccgcg agccggcggg cggcatcccg cccccggcgt ccccgctgaa gaaggtccgg
    12061 aagaagtcgg agaagtcgcc gccgccggcc gtccggaact cgtagcggac gttgccggac
    12121 cggccgccgc ccgccccgaa gccgccgaac gggctgccgc cggcgaaggg tccgccggcc
    12181 gcgtcgggat cgacgcctgc ccgcgagtac gcctcccaat ccttgccgag tcggtcgtac
    12241 ttcttgcgct tgtcgggatc cgagaggacc gcgttggctt cgttgatcgc cttgaagcgc
    12301 gcctcggcgg ccttgtcgcc gggcttcttg tcggggtggt gctcgcgggc gagcttgcgg
    12361 aaggccttct tgatgtcggc ctggctggcg ccccgcgcga ccccgagggt ctggtagtag
    12421 tcctggaagt ccatcgcccc gggaatgacc tcagaaaagg aacgaggccg gtgatggatc
    12481 accggccccg tcgcgtcgtt gctctagcgg tgccggtcga cgacgaccgg gggctgatcc
    12541 gtcggcggga aggacgtccg gcggacgcgt tcctcccgtt ccggcgcatc gctcggtgcg
    12601 tcgccgggct tgacgaccgt tggcggctgc gcgatcacga gcgccttcgg cttgcgccgg
    12661 agggccgtct cgaggacctg ctccatcgag tcgacaagga caagcttcat cgacttgcgg
    12721 atctcgtcgg ggatgtcccg gaggtccttc tcgttcttgc gtggcaggat caccatcttc
    12781 gccccggcca ggtgggcggc caggatcttg ctcttcagcc cgccgatcgg aaggacccga
    12841 ccgcggaggg tgatctcgcc ggtcatcgcg acgtccttgc ggacggggat cccggtcagc
    12901 gcgctgacga tcgcgagggc catcgtgatc ccggccgacg gaccgtcctt gggcgtgccg
    12961 cccgccggaa cgtggatgtg gagcgtgttc ttctcgaaga tctcccgcga gatgcccagc
    13021 tcggttgcgt gggcgcgcgt ccacgacagg ccggcccggg cggactcgcg catcacgtcg
    13081 ccgagctggc cggtgaggat gaagtcctcc ttgccttcca tcttcgtgac ttcgatcgcg
    13141 atgacgtcgc cgccgacctc ggtcacgacg agaccggtcg ccgagccgat ctggtcctcg
    13201 gcctcgagct cgccgtactc gtagcgcggc gggccgaggt actcggcgag gcgtttgagg
    13261 tcgatgatcg tcttgcgctt gcggccctcg gccacggtcc gggcgacctt gcgcatgacg
    13321 ttcgccagct cccgctcgag gttccggacg ccggcctcgt gggtgtagga gcggacgagc
    13381 tcgatcatcg tcccgtcggg gatcacgatg ttcttcccgg acaggccgtg gttcttcatc
    13441 tgcttgggga tgaggaaccg cttgccgatc tcgatcttct cctgctcggt gtagccgggc
    13501 aggtagacga tctccattcg gtcgcggagc gcggccggaa tcgggtcgag gaggttggcc
    13561 gtcgcgacga agagcacctt cgagaggtcg aacgggacct cgaggtagtt gtcctggaag
    13621 gtgttgttct gctccggatc gaggacctcg aggagggcgg acgacgggtc gccccggaag
    13681 tccatcccga tcttgtcgac ctcgtcgagc atgaagaccg ggttgttgga ccccgccgtc
    13741 ttgatgttct ggatgatccg cccgggcagg gcgccgatat aggtccgacg atgaccgcgg
    13801 atctcggcct cgtcgtggat gccgccaagg ctcatccgga cgaacttgcg gcccatcgcc
    13861 ctggcgatgc tcttgccgag cgaggtcttg ccgacgccgg gcgggccgac gaaggccagg
    13921 atcgggctgc ggatctggtc cgccagggtc cggaccgcga ggtactcgag gatccgctcc
    13981 ttgatcttct ccaggccgta atgatcctcg tcgaggatct ttgcggcctg cttgatgtcg
    14041 agctggtcat cggtcgcgac gttccacggc aggccgacca gccagtcgac gtaggtccgg
    14101 atcacgccga cctcggggga cgcggacggg atccggctca tccggtcgac ttccttgatc
    14161 gcccgggtct tgatctcctc gggcatgccg gccgcttcga ccttgtcgcg gagctcgttg
    14221 atctcggcct gctgcgggtc gtcctcgccc agctcgcgct ggatcgcctt gagctgctcg
    14281 cggaggatgt actcgcgctg ggtcttgtcc atctcggact tgacctcgga ctggatccgg
    14341 cccttgagct cgaggatctc gatctggcgg gcaaggaagt tgctgacgag cttgagccgc
    14401 tctttcacgt cgaccgtctc gagcagctcc tggcgctgct cggtgctcat gtcggggctg
    14461 taggcgacca tgtcggccag gagcccgggc tcgctgatgt tgcgggccgc gacggcggcc
    14521 tccggcggca ccggtgcacc gttggcgacg tactgctcga tctgggcctg gacggtccgc
    14581 atcagggcct ggacctcgag gctggcgccg ctggcgtcgt ggagctcctc gacggtcgcc
    14641 cggagatacg gctcgttggc cacgaagttg atgagcttga ggcgggtctg gccctggacg
    14701 atggcccgga ctgtgccgtc ctggagctgg acgacctggg cgatcttcgc cagggtcccg
    14761 acggtataca gctcggacgg ggagtcgatg tcctcctgct cgggctggcg ctgggtgacg
    14821 agggcgatcg gaccgccctc ggcaaccgcg gcgttgagcg cgttgacgct cttctcgcga
    14881 ccgacctgga gcggaacgat catctcggga aagatgacgg tctcgcggag ggcgacgagg
    14941 ggcagctcgc gcggaccgct tggcgtcgtg atgttcacgg tggtcggctg atcgtcccgg
    15001 tcttgggtcg ggcgctttgc catcaaaggt ctcctgtgag atgtaacgag atcatcgggc
    15061 ctgcgcggcc gctcgtgaag tagggcttcc tctggttcgt catgaccgat cgtccggtac
    15121 ggtcccggca cgatcgggct tcggggacgc ctcggcggcg gcccgatcgg cggcctggcg
    15181 acgcgtgccc tcggcgtaca gcgcttcgag gattcgcgtc cccagccgct cctccagctc
    15241 gaacaggagc cggatgccgg cgaggttgac gcctcgatcc tgggtcaggt gccggatcca
    15301 ggtgaggcgc cggatgtcct cgtcgctata gaggcggatg ttggacgggg tgcgggcggg
    15361 acagatcagg ccttcgtcct cgtagatccg gagcgtccgc ggatgggcgg cgacgagcgt
    15421 tgccgcgacg ctgatcacgt agaccggacg ccgcgggtcc ttctcgaccc ggccctggga
    15481 catgcgctca ggctccctgg ccggtgccgg cggcgacggg ggtcttcccg ttgccggtcg
    15541 tctcggccgc ccggacctcg gcggaggcgc cgttgaccgt cgggctgatc cggatctggc
    15601 ggggcttgac cgactcggcc ttcgggatcc gcagcgtcag gacgccgttt tcgaaggtgg
    15661 cggtcgcctt gtccggctcg agcccggtcg ggaggatgat cgcccgcccg accgtcccgc
    15721 gacggatctc ggagatgagg gcctcgccct cgcctgcctg ctgcacgtcg cgatcctcgc
    15781 cgcggatcga cagggtgcgg tcctcgacgg tgatctcgac gtcctccggc cggatgccgg
    15841 gcaacgccgc ctcgatcacg agcgcgtcag ccgtacgggt gacgtcaagg ggaagctggg
    15901 ttggagcatc gaaaccggcg ccgaacgggc gtcggacgaa gctgtcatcg aacaatcgat
    15961 ccatcgccga gcggagcgac aagagctcgc cgaacggcga gggacggcgc acgatcgtca
    16021 tgttcgtgga ccctcctttg gtgtggtgcc atggcgcccg acggacagtc ccggcggaca
    16081 acgagaaggc tagaaccctg acaatcacgt tgtcaagaag gttgcgattt gttgacgatg
    16141 agacttgccc gcgccgcttg ctggccccag gatccggccg tcccgaactg gcgcgcgcat
    16201 acccgcctgc cttcgtgtcg gcgtcgccgg gatttcctgc cctgagccga gacctgcctg
    16261 gtgtccagga cctcggggat cgtcggccgg ccctccgagg tcagccggcg ggtgcggcct
    16321 cctggtcgcc gatgagctgc tcgtggagct gcggtccgct ttccgccttg ccgatcgcga
    16381 tcaccttgtc gccttcggcg aggaccgtct cgggtcggag cggtgtcgcg acgccgtcgc
    16441 ggatcaccgc gaacagcgag cagccgtccg gcatggacag gtcgcgcggt gtcttgccga
    16501 tggccggcga gccggcctgg agatgggcct cgatcagctc cagctcgccc tcgccgaggg
    16561 cagccaggtg gaggagctcg tggacgggga tgtcctgctc gatcgagccg aggatcatcc
    16621 gggtcgggct gatcagttcg tcgacgccga ggtgcttgaa cagggtctcg ttcttggggt
    16681 tgttgacccg ggcgatcgtg cgcgggacgt cgaaatggtg cttcgccatc tggcagatga
    16741 cgaggttgtc ctcgtcgtcg ccggtcacag cggccacgat gtccgcgcgg ttggcacctg
    16801 cctggccgag gtacttgccc tcgcagccgt cgtgggcgat gacgaccgat ccgatctcgt
    16861 cggcgatctg gttcgcccga ttctgttcct tctccatcag gacgacctcg tggccgctgc
    16921 cgatgagctc cttggtcagg tagtagccga cctttccgcc gccgatgacg agcacgaaca
    16981 cgggtctcag ccctcccggg cgctgatggg cgcgaccggc gccggagtcg gatcggtcgg
    17041 agcggcgaac gccgttgcgg ccggcaacga ttcgtcgagc ggaccgtggt gttctccgcc
    17101 cgggtgatgg ccggtggccg ccgacaggcc cggcccgaag gtcaggccga ggccgacgaa
    17161 gtcgctgacc gcgctggcca tcaggttcgt ccggcagagc gtggcgactc cgagctcggc
    17221 gtacgcgctg gccctgagcg ggtcgttgat cttggcgatc acctgcttcg cgtcgagcgc
    17281 ctcggtcgcg agctgggccg acatgatgtt gcggttgtcg ccctcggtca tcgccatgaa
    17341 caggtcggcc gactccgcgc ccgcccggcg gagagtgtcc tcgtcggtcc cgtcgccgcg
    17401 gatagcggcg cccttgaacg tgcccggcag gcggtcgaat gcggtcgtat tcgggtcgag
    17461 gatgatgacc tggtgaccgg cccggtcgag ggcgtcggcc aggacggacc cgacgcggcc
    17521 gcaaccgatg atgacggtct tcacgatgtc tcctccggga tcggctcgcg cacgacccag
    17581 actgcgcagg gagcgttctt gaggacgtac gggatcgtcc gcccgatctc gaagtccccg
    17641 ccgaacttgg tccggtaggc gaggccgatg acgaggaggt cggcttcgcg ctcgacggcc
    17701 tcgtcgacga gggcggcccc gacgtcacgg gcctggagca ggacgggctc gagacggacc
    17761 ccggacgact cggccgtgcc ctcggccagg tcgaggaccc gctggatctc ctccgatcgc
    17821 ccggcaacgt ccatgtcgag cggcaccgac cagtccatct ccacgacgtg gacgccgatc
    17881 agctccgcct tgtgggcctt cgccagctgc gcgacgaggc ggacaagccg ctggccgttc
    17941 gggtggccgg agagtgcaac gacggctcga cgaaacgacg gatgagcttg ggactcgggt
    18001 ggcacggcgg cgactgtacc accgccacgc cacggcgtcg cccgtccgac gagcgttccc
    18061 gttgcaggga cgtcacggct ttgcggactg ctcgtcgccg gcggatgggt ggccgcgctc
    18121 gaccatcgtc gggtcatctc ggcggtacgg gacgttgacg acgaccgtgt gtggccggcc
    18181 gaggagcgag ctccgcaggc gcttcaccga ctggttgtag aggatccgct cccaccaccg
    18241 gcgagccacg tactcgggca ggacgacgaa cgtgatgggc gcctccttgt ccggtggcca
    18301 ggccgagtcc agcacgtcga ggtaggcccc gagcgggccg gtcagtgccc gatacggcga
    18361 ttccacgacg acgagcggga cgccggggac ctggcgctcc cattgttcgc gcaccgcgag
    18421 gccgacctct gcatcctcgg tgatgaagac ggcccggaca tcgtcggcga tggaccgggc
    18481 gacattgacg gcgtgaatca ccgcccggtt gacgcccggg atcggcacga ccgcccgctc
    18541 ctcacgatgg aggctggtga agaccttgtc cggccggacg ctcagctcgc gccgcgaggc
    18601 gtcgtactgg cgatggatga acagcatcat cgcgacgagg gtcgggatca ggatgaccac
    18661 gaggtacgcg ccgtcgacga acttgacgct gacgacgacg acgagcacga ccgcggtcag
    18721 gacggcgccg aaggcattga tcccgagccg ccactgccag cccgacccgt gctccgatcg
    18781 ccagtgcttg accatcccga tctggctcag ggtgaagcag acgaacacgc cgaccgagta
    18841 gagcgggatc agggccgtga cgttcccctc gaacagccag atcaggaacg ccgccaccgt
    18901 ggacaggacc acgatccccc acgagtaggc gagacgatcg ccgcggtagg agaactggcg
    18961 cggcatgtag ccgtcgaccg cgaggatcgc gcccagtcgc gggaacgcgt tgaacgaagt
    19021 gttcgcggcg aggaacagga tcagcgcggt cgccgcctgg aacaggacga acaggagcga
    19081 gccgttcccg aacacgttgc cggcgacgag cgagaccgtt gtcgggatgt cgccgaccgt
    19141 cggcaggatc tggtaggcgt gggagatgat cgtgatcccg atgaagagga tcccgagcag
    19201 gatcgccatc gcggtcatcg tgttggccgc gttcttcgct tcgggcggct tgaaggccgg
    19261 cacgccgttg gcgatcgcct cgacgccggt cagggcgacc gaccccccgg cgaatgcctt
    19321 gagcagcagg aagatcgaca gctcaccgcc gctgacgacc tccgcattgg gcgtgacgat
    19381 cgggtgggcg ttgccggtca cgatgttgac gatgcccagc ccgaccatca gcagcgccag
    19441 gccgacgaag gcgtaggtcg ggaccgcgaa gatgttcccg gactcgcgca ggccgcgcag
    19501 gttggcgacc gtcatcagga cgatcaccgt gatcgcgatc tcgatcttca cgccgtcgag
    19561 gtccggcacg accgacacga tctggccgat cgccgacgcc gtcgacaccg cgacggtcat
    19621 cacgtagtcg atcaggaggg ccgcggccgc gacgaggccg agcagcgggt tcaggttctc
    19681 cttggccacc gcgtacgcgc cgccgccgcc cgggtaggct cggcacacct ggcggtacga
    19741 cgtcgcgacg accgcgagca ggacggcgat cgcgatcgac acaccgaccg agaaggcgag
    19801 tgccgtcgcc ccggcaaccg cgaggacccg gatgatctcc tgcgtcgcgt aggccgacga
    19861 gctgatcgcg tcggagctga agatcgcgag ggccttcttc ttcgtgaggc gctcgccggc
    19921 ctcctcctcg gacgccaggg ggcggccgat cgcgaccttc cggatccggg cccaggtccg
    19981 cccgaccgcg gtcttcgggg tgtgggcggc cgctttcgcg accatctggc cggggcccgt
    20041 gtagcggaaa tacggggcat gcggtcgatc gacacgaacg cggcgatccg cgggtttccg
    20101 gccctgcaac ggccggcggc ctccgatcat ccgactccga tctgttgggc tggggggccg
    20161 gccagtggac gcggcgtagg ggacgtccgc gatgaccgtg tgatcgcgcc cgacgagcgc
    20221 cgatttttcg ggcgtctggc cgactggtta tacagcagac ggtcccacca gtgccgggcg
    20281 acgaattcgg gcaggacgac gttcgtggtc ggggctggct tgtccggcgg ccaggacgag
    20341 aacgagcaca ggaactggcg tcgcatgtga ccgtcgatgg ccaggatcgc gagcagccgg
    20401 ggaaacgcgt tga
//
LOCUS       DATAIW010000446         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_253871, whole genome shotgun sequence.
ACCESSION   DATAIW010000446 DATAIW010000000
VERSION     DATAIW010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253871"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1125)
                     /locus_tag="VHM48_13355"
     CDS             complement(70..1125)
                     /locus_tag="VHM48_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2756449.1"
                     /translation="MRLERLTPGRRPPAELVGAVLARDLVVGGSRWSKGRRLSVADLA
                     AWAVDPSLGVGPVTVIVLDEGDLHEDDAALRLADAVAGSGLTRRGPSQSRVDLLAEHD
                     GVLHVRATELERLNRIDPLEVFTRLDGSIVAAGDLVASVKVAPHVVPASVVEAGEQVV
                     AGGRRPLVRVAPFRPMRVGVIVKESLRAADRPRFEASVRVKVESLGSTIVGIEYVGDE
                     DGAVLAALSRQVRGRAGSAVDVVLTAGGRSTDPSDPFFTAIEALGGRVVRHGVPAHPG
                     SMLWLARVGRTAILGLPTCGAYSKATAADLLLPRLLTGEPPTLRTVSHLGHGGVLTRE
                     MRFRFPPYARGLDAPDG"
     gene            complement(1168..>1677)
                     /locus_tag="VHM48_13360"
     CDS             complement(1168..>1677)
                     /locus_tag="VHM48_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX2756450.1"
                     /translation="DPASDLPAVLLALDAEIVARSKRGEHVYAADGFFTGAFTTALAE
                     DELVTEIRLPGPSDHPGSAFVSLSQRASGYSIVGVAAVVFKEGGSAITRAGIALTGVA
                     DAPYRARAVEAVLIGNEGTAESIAAAAAHATDGMTVHGDIHADREYRTAMAVTYTKRA
                     IAAALERLG"
BASE COUNT          225 a          626 c          637 g          189 t
ORIGIN      
        1 atgctacacc gcgacccggc cgtccgggcg tggcgtcggc gcccgaacgg agggcggagg
       61 cggcgtgact cagccgtcgg gtgcgtcgag cccgcgcgcg tagggcggga agcggaagcg
      121 catctcgcgg gtcaggaccc cgccgtggcc gaggtgcgac accgtccgga gggtcggggg
      181 ctcgccggtc aggagtcgcg gcagcaggag atcggcggcg gtcgccttcg agtaggcgcc
      241 gcaggtcggc aggccgagga tcgcggtccg cccgacgcgg gcgagccaca gcatggagcc
      301 cgggtgggcc ggcacgccgt ggcggacgac gcggccgccg agggcttcga tggccgtgaa
      361 gaatgggtcc gacgggtcgg tcgagcggcc gcccgcggtc aggacgacgt cgaccgccga
      421 gcccgcccgg ccgcggacct gccggctcag cgccgccagc accgcgccgt cctcgtcgcc
      481 gacgtactcg atcccgacga tcgtcgaccc gaggctctcg accttgaccc ggacgctcgc
      541 ctcgaaccgc ggccggtcgg cggcccgcag ggactccttg acgatgaccc cgacccgcat
      601 cggccggaac ggcgcgaccc ggaccagcgg ccggcggcca ccggcgacga cctgctcgcc
      661 cgcctcgacg accgatgccg gaacgacatg cggcgccacc ttgacgctcg cgacgaggtc
      721 gcccgcggcg acgatcgagc cgtcgagccg ggtgaagacc tcgagcgggt cgatccggtt
      781 gagccgctcg agctccgtcg cccggacgtg gaggaccccg tcgtgttcgg cgaggaggtc
      841 gacccggctc tgcgacgggc cacggcgcgt gagccccgat ccggcgacgg cgtcggcgag
      901 acggagcgcc gcgtcatcct cgtggaggtc gccctcgtcg aggacgatga ccgtgaccgg
      961 gccgacgccg agcgaagggt cgaccgccca ggcggcgagg tcggccaccg acagccgccg
     1021 gcccttcgac cagcggctgc ccccgaccac gaggtcgcgc gcgaggaccg ccccgacgag
     1081 ctcggcgggc gggcggcgac ccggcgtcag acgctcgagc cgcacccgcc gacgcgccct
     1141 ggaaccgctg cggccgcgac gcgcgttcta gccgagccgc tcgagggccg ccgcgatcgc
     1201 ccgcttcgtg tacgtcacgg ccatcgccgt ccggtactcc ctgtcggcat ggatgtcgcc
     1261 gtggacggtc atcccgtcgg tcgcgtgggc ggccgccgcg gcgatcgatt cggctgtccc
     1321 ctcgttgccg atcaggaccg cctccaccgc ccgcgcccgg tatggcgcgt cggcgacgcc
     1381 ggtgagcgcg atcccggccc gggtgatggc gctgccgccc tccttgaaca cgaccgccgc
     1441 gaccccgacg atcgagtagc ccgaggcccg ctgcgacagc gagacgaacg ccgaaccggg
     1501 atggtcgctc ggtcccggca accggatctc cgtcacgagc tcgtcctcgg cgagggcggt
     1561 ggtgaaggcc ccggtgaaga agccgtcggc cgcgtagacg tgctcgccgc gcttcgaccg
     1621 ggcgacgatc tcggcgtcga gggcgagcag gaccgcgggc aggtcggatg cgggatc
//
LOCUS       DATAIW010000447         6404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_284025, whole genome shotgun sequence.
ACCESSION   DATAIW010000447 DATAIW010000000
VERSION     DATAIW010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284025"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VHM48_13365"
     CDS             complement(<1..1158)
                     /locus_tag="VHM48_13365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2756451.1"
                     /translation="MPTANHSLRIIPLGGVGEIGKNMYVFEYGDDIVVIDCGLMFPDE
                     EMFGIDLVIPDITYLKENKAKVKAFLITHAHEDHVGALPYVLPEFPGVPVYASTLARG
                     LLGNKIKEHKLHNNPILALEPGDELQIGPFGVVAFRIGHSIPDAMGIALRTPAGVIVH
                     TGDFKFDHTPVDGKLSDFAILAKLGAEGVVCLLSDSTRAENPGYTPSERTVGEAFREI
                     MEPLDGRVIVATFASNIARVQQVLDAAASFGRKTTVIGRSMEQNFRIATDLGYLTYDP
                     SMIVAKDKLNEIPADKLVICTTGAQGEPMAGLARMANRDHRFVEIQQGDTVIVSASPI
                     PGNEEYVSRTIDNLFKAGANVFYHTIKRAHVSGHASQEELKLMLGLTKPKYF"
     gene            complement(1191..1913)
                     /locus_tag="VHM48_13370"
     CDS             complement(1191..1913)
                     /locus_tag="VHM48_13370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2756452.1"
                     /translation="MTSAERQAALETVAAEVRTCTRCRLAEGRTNAVPGEGSPDTEVV
                     LVGEGPGFNEDRLGRPFVGRAGDLLVKLLGSIEWRREDVFITNVVKCRPPDNRDPEPD
                     EVAACAPYLKRQLEVLDPALIVTLGRHSMGRFMPGARISQAHGTMGPVDPASGARDAT
                     VFAMYHPAAALRGVEVERQSYDDIAKVPQVLLDLRARRATSTSERPAPADNGAMHGAE
                     PDGTPALALASAPEPAADITIF"
     gene            complement(1910..3001)
                     /locus_tag="VHM48_13375"
     CDS             complement(1910..3001)
                     /locus_tag="VHM48_13375"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX2756453.1"
                     /translation="MTRPSFTVAVVGATGVVGRTMIQVLLERDFPVGELRLLASERSA
                     GRMISIEGRTLEVQPSSADAFEGVDIALFSAGASASRELAPLAASRGATVVDNSSAWR
                     MDPLIPLVVSQVNPDDLEGHPGIIANPNCSTMQLVPVLMALRDAVGLERVVVDTYQAV
                     SGTGGEAIRELEGQIRAHVAGEPKTVAVYPHQIAFNALPQVDVFLPSGYTKEEWKVVT
                     ESRKILHLPELRVSCTAVRVPVFVAHSEAVHVETRAPIDPDRARELFAAVDGVVVQDD
                     PSTSTYPLATSAAGSDDIFVGRVRQDPSIDDNRGLAFWVVSDNLRKGAATNTVQIAEV
                     LVERGWVRSAASRGATPYHAEGTAIGAPA"
     gene            complement(3396..5978)
                     /gene="pnp"
                     /locus_tag="VHM48_13380"
     CDS             complement(3396..5978)
                     /gene="pnp"
                     /locus_tag="VHM48_13380"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX2756454.1"
                     /translation="MSQTFETQIGGRTLTIETGKLARLAGGAVTVRYGDTFILGTANR
                     SDPRPGLDFFPLTVEFEERMYAAGKIPGGFIKREARASEAATLAARLTDRPIRPLFPE
                     GYKDDIQLVITVLSTDQENEPDVLGTVAASAALTISEIPFAGPVGAVRIGRIDGEFVV
                     NPTYSELADSELDLIVSGTRDAIMMVEAGAKLLPEDVMAEAILFGHRALQPLIDIQEQ
                     LREAVGKPKRSPYLEPGTGSVLDFVAAVDAKREFAIVDVETTGTDPKMADLLEVAVVK
                     VKGGKITDRWSTFVKPSQPILGNQMHGITDKDVAKAPSPAEAAGQAVDFIGDALVVGH
                     SVIFDLGFLEEAGAARYEQGRYLDTLTIAREGYPDLQNYKLDTLAGFFGVDLTQNHRA
                     LPDAEATANLLLKFAADLPGRIATLKSEISDSVRANKTSKDEAKTKLEAARRKARVSK
                     SLWSLVYKKTCRTLALDEGVRMDGRGTTELRQISVEVGLLPRAHGSGLFTRGETQVMT
                     IATLGASSDVQRIDTISPKTEKKYIHHYNFPPYSTGENKPMRGPSRRDIGHGNLAERA
                     LIPVLPPHDEFPYVIRLVSECMSSNGSTSMASTCGSTLALMDAGVPISAPVAGAAMGL
                     ISEPDGRFVVLTDILGKEDSVGDMDFKVTGTRDGITALQMDIKVQGINEAIIRDGLKQ
                     ALAARLEILDKMTEVMPEARGSMSDFAPRITTIKINPEKIREIIGKGGSMIRKIQEET
                     QTEINVEDDGTVEIAAVSGENSRKAIQWIESLTREVEVGALYLGKVTRIMGFGAFVEI
                     LPGKEGLVRIGELADYHVPTVEDVVSVGDEVMVVVMEVDRQGRVNLSRKAAMQRHLAK
                     EPVS"
     gene            complement(6101..6370)
                     /gene="rpsO"
                     /locus_tag="VHM48_13385"
     CDS             complement(6101..6370)
                     /gene="rpsO"
                     /locus_tag="VHM48_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2756455.1"
                     /translation="MPLAREQKQEIMTGYATKEGDTGSPEVQIALLTERIRSLTDHLR
                     GFPKDNHSRRGLLKLVGQRRRLLAYLIKKDNTRYRTVIGRLGLRR"
BASE COUNT          930 a         2065 c         2311 g         1098 t
ORIGIN      
        1 gaagtacttc ggcttggtca gcccgagcat cagcttgagc tcctcctggc tggcgtgacc
       61 ggagacgtga gcccgcttga tcgtgtggta gaagacgttc gcgccggcct tgaacaggtt
      121 gtcgatcgtg cggctgacgt actcctcgtt gcccgggatc gggctggcgc tgacgatcac
      181 cgtgtcgccc tgctggatct cgacgaagcg atggtcgcgg ttggccatcc gggcgaggcc
      241 ggccatcggc tcaccctggg cgccggtcgt gcagatcacg agcttgtcgg ccgggatctc
      301 gttgagcttg tccttggcca cgatcatcga cgggtcgtac gtcaggtagc cgaggtcggt
      361 cgcgatccgg aagttctgct ccatcgagcg gccgatgacg gtcgtcttcc ggccgaacga
      421 ggcggcggca tcgagaacct gctggacccg ggcgatgttg ctcgcgaagg tcgccacgat
      481 cacccggccg tcgagcggct ccatgatctc gcggaaggcc tcgccgaccg tccgctcgga
      541 cggcgtgtag cccgggttct cggcgcgggt cgagtcggac aggaggcaca cgacgccctc
      601 ggcgccgagc ttggcgagga tcgcgaaatc ggacagcttg ccgtcgaccg gggtgtggtc
      661 gaacttgaag tcgcccgtat ggacgatgac cccggccggc gtccgcaggg cgatccccat
      721 cgcgtccggg atcgaatggc cgatccggaa ggcgacgaca ccgaacgggc cgatctggag
      781 ctcgtcgccg ggctcgaggg cgaggatcgg gttgttgtgg agcttgtgct ccttgatctt
      841 gttgccgagc aggccgcggg cgagcgtcga ggcgtagacg gggacgcccg ggaactcggg
      901 caggacgtac ggcagagcgc cgacgtggtc ctcgtgggca tgggtgatga ggaaggcctt
      961 gaccttggcc ttgttctcct tgagataggt gatgtcgggg atgacgaggt cgatcccgaa
     1021 catctcctcg tcggggaaca tcaggccgca gtcgatgacg acgatgtcat cgccgtactc
     1081 gaacacgtac atgttcttgc cgatttcgcc gacgccgccg agcggaatga tccggaggga
     1141 atggtttgct gttggcatgg gagggtgttt cgtccttcgt tggtggttga tcagaagatg
     1201 gtgatgtcgg ccgcaggctc cggcgccgag gccagcgcga gggcgggtgt gccgtcgggc
     1261 tcggctccgt gcatggcccc gttgtcggcc ggtgcaggcc gctcggaagt cgaggtcgca
     1321 cggcgggctc ggaggtcgag gaggacctgc ggaaccttcg cgatgtcgtc gtagctctgc
     1381 cgctcgacct cgaccccgcg gagcgcggca gccgggtggt acatcgcgaa cacggtggcg
     1441 tcgcgggcgc cgctcgccgg gtcgaccggg cccatcgtgc cgtgggcctg gctgatccgg
     1501 gcccccggca tgaagcggcc catcgagtga cggccgaggg tgacgatcag ggccggatcg
     1561 aggacctcga gctggcgctt caggtacggc gcgcaggccg cgacctcgtc cggctccggg
     1621 tcgcggttgt ccggcggccg gcatttgacg acgttggtga tgaagacgtc ctcgcgccgc
     1681 cactcgatgg agccgaggag cttgacgagc aggtcaccgg cgcggccgac gaacgggcgg
     1741 ccgaggcggt cctcgttgaa gccgggcccc tcgccgacga ggacgacctc ggtatcaggg
     1801 ctgccctccc ccggcacggc gttggtccgg ccctcggcga gccggcagcg ggtacacgtc
     1861 cggacctccg ctgcgaccgt ctcgagcgcc gcctggcgct cggccgaagt cacgccgggg
     1921 ccccgatcgc ggtcccctcc gcgtggtacg gcgtcgcacc ccggctcgcg gccgatcgca
     1981 cccagccgcg ctcgacaagg acttccgcga tctggaccgt gttcgtcgcg gcgcccttgc
     2041 ggaggttgtc gctgacgacc cagaaggcga ggccgcggtt gtcgtcgatc gaggggtcct
     2101 ggcggacccg cccgacgaag atgtcgtccg agcccgccgc ggaggtcgcc agcgggtacg
     2161 tcgaggtcga cggatcgtcc tggaccacga cgccgtcgac ggcggcgaac agctcccgtg
     2221 cccgatcggg gtcgatcgga gcccgggtct cgacgtggac cgcctccgag tgggcgacga
     2281 agaccgggac ccggacggcc gtgcacgaca cccgcagctc tggcaggtgg aggatcttgc
     2341 ggctctcggt gacgaccttc cactcctcct tcgtgtagcc gctggggagg aacacgtcga
     2401 cctggggcag cgcgttgaag gcgatctggt ggggatagac ggccacggtc ttcggctcgc
     2461 cggcgacgtg ggcccggatc tggccttcca gctcgcggat cgcctcgccg cccgttccag
     2521 acaccgcctg gtaggtgtcg accacgacgc gttcgagccc gaccgcgtca cggagggcca
     2581 tcaggaccgg gacgagctgc atcgtcgagc agttggggtt ggcgatgatc cccgggtgtc
     2641 cctcgagatc gtcggggttc acctggctga cgaccagggg gatcagcgga tccatccgcc
     2701 aggccgacga gttgtcgacg acggtcgctc cacgcgaggc ggccagcggc gcaagctcgc
     2761 gcgatgcgct ggcgccggcc gagaacaggg cgatgtccac gccctcgaat gcgtccgcgc
     2821 tcgacggctg gacctccagc gtccggccct cgatcgagat catccggccg gccgaccgct
     2881 ccgatgcgag cagccgcaac tcgccgacgg gaaagtcgcg ttcgaggagg acctggatca
     2941 tcgtgcgccc gacgacgccc gtggcgccga cgaccgcgac ggtgaaggat gggcgtgtca
     3001 tgtcgggcat ggctccgctg tcactgtcgc tggcagggca cccacggagt atagcagcgg
     3061 ggtcggccgc ggctgagggc cggacccctc cgctcgtgcc ggttgctccg atcccgttcc
     3121 gtcctgccag ataccacgcc aggtagtggc agtcatggcc cgagggccat ccggagtccg
     3181 agccgaccga ttcgggcccg gctgcgcgcc actcaccgcc cgcgacggtg aacggcgaca
     3241 acggccgtcc ccgacctcga cgctcaccac gactggtggt gcatgacagg gccgacaggc
     3301 gagtcggggc cagggccgcg cgtggatctg gtcggaatcc gaagggccgg cccgtcggac
     3361 cggcccttcg cgtttcagcg ggttcgcggc ggcggtcagc tcacaggctc cttcgccagg
     3421 tgcctctgca tggccgcctt gcgggacagg ttgacccggc cctggcgatc gacttccatg
     3481 acgacgacca tcacctcgtc gccgacggac acgacgtcct cgacggtcgg cacgtggtag
     3541 tcggccagct cgccgatccg gacgaggcct tccttgcccg gcaggatctc gacgaacgcg
     3601 ccgaagccca tgatccgggt gaccttgccg aggtacagcg ccccgacctc gacctcgcgg
     3661 gtgaggctct cgatccactg gatcgccttg cgcgagttct cgccggagac ggccgcgatc
     3721 tccacggtgc cgtcgtcctc gacgttgatc tcggtctggg tctcttcctg gatcttgcgg
     3781 atcatcgagc cgcccttgcc gatgatctcg cggatcttct ccgggttgat cttgatcgtg
     3841 gtgatccgcg gcgcgaagtc gctcatcgag ccacgggcct cgggcatgac ctcggtcatc
     3901 ttgtcgagga tctcgagtcg agcggcgagg gcctgcttca ggccgtcgcg aatgatcgcc
     3961 tcgttgatcc cctggacctt gatgtccatc tggagggcgg tgatcccgtc gcgggtgccg
     4021 gtgaccttga agtccatgtc accgaccgag tcttccttgc cgaggatgtc ggtcaggacg
     4081 acgaaccggc cgtcgggctc gctgatcagg cccatcgccg cgccggcgac cggagccgag
     4141 atcggcacgc cggcgtccat cagggcgagg gtcgagccac aggtcgaggc catcgaggtc
     4201 gaaccgttcg acgacatgca ctcggacacg aggcggatga cgtacgggaa ctcgtcgtgc
     4261 ggcgggagga cagggatgag cgcccgttcg gcgaggttgc catgaccgat gtcgcgccgg
     4321 ctcggcccac gcatcggctt gttctcgccc gtggagtaag gcgggaagtt gtagtggtgg
     4381 atgtacttct tctcggtctt cgggctgatc gtgtcgatcc gctggacgtc cgaggacgcg
     4441 ccgagggtcg cgatcgtcat cacctgcgtc tcgccgcggg tgaagagacc ggagccgtgg
     4501 gcccgcggga gcaggccgac ctcgaccgag atctggcgga gctcggtcgt cccgcgaccg
     4561 tccatccgga caccctcatc gagggccagc gtccggcagg tcttcttgta gacgaggctc
     4621 cagaggctct tgctgacacg ggccttgcgg cgggcggcct cgagcttggt cttggcctcg
     4681 tctttcgagg tcttgttggc ccggaccgag tcggagatct cgctcttcag ggtcgcgatc
     4741 cggcccggca ggtcggcggc gaacttcagg agcaggttgg ccgtggcctc ggcgtccggc
     4801 agggcgcggt ggttctgggt caggtcgacc ccgaagaatc cggccagggt gtccagcttg
     4861 tagttctgga ggtccgggta gccctcgcgg gcgatcgtca gggtgtcgag gtagcggccc
     4921 tgttcgtagc gggcggcgcc ggcctcctcg aggaagccga ggtcgaagat cacgctgtga
     4981 ccgacgacga gggcgtcacc gatgaagtcg accgcctgcc cggcggcctc ggccggcgac
     5041 ggcgccttgg cgacgtcctt gtcggtgatg ccgtgcatct ggttgccgag gatcggctgc
     5101 gacggcttga cgaacgtcga ccagcgatcg gtgatcttgc cgcccttgac cttgacgacg
     5161 gcgacctcga ggaggtccgc catcttgggg tcggttccgg tcgtctcgac gtcgacgatc
     5221 gcgaactcgc gcttggcgtc gaccgcagcc acgaaatcga ggaccgagcc ggtgccgggc
     5281 tcgaggtacg gcgagcgctt gggcttgccg acggcctcgc ggagctgctc ctggatgtcg
     5341 atgagcggct ggagcgcgcg atgaccgaag aggatggcct cggccatgac gtcttcaggc
     5401 aggagcttgg ccccggcctc gaccatcatg atcgcgtcgc gcgtcccgga gacgatcagg
     5461 tcgagctccg agtcggcgag ctcgctgtag gtcgggttga cgacgaactc gccgtcgatc
     5521 cggccgatcc ggacggcgcc gacgggaccc gcgaacggga tctcgctgat cgtcagggcg
     5581 gccgacgcgg ccaccgtgcc gaggacgtcg ggctcgttct cctggtcggt cgagaggacc
     5641 gtgatgacga gctggatgtc gtccttgtag ccctcgggga acagcggccg gatcgggcgg
     5701 tcggtcaggc gggccgcgag ggtggccgcc tccgaggcgc gcgcctcacg cttgatgaag
     5761 ccgcccggga tcttgcccgc ggcgtacatc cgctcctcga attcgaccgt cagcgggaag
     5821 aagtcgaggc cgggccgcgg atcggaccgg ttggccgtcc cgaggatgaa agtgtcgccg
     5881 tagcggaccg tgacggcccc gccggcgagt cgggcgagct tgcccgtctc gatggtcagc
     5941 gtgcgaccac cgatctgtgt ctcgaatgtc tgagacaagg gaatgttcct ccttggtcgt
     6001 ggaggaagga aacgacccgc gggcaggtcg gccgtgctgt gcctggctga acgtcgaatg
     6061 cgaacgaacc ccggatgtcc cggggtcccg tgcggtgacg ctagcggcgc agtccgagcc
     6121 gtccgatgac ggtccggtat cgcgtgttgt ccttcttgat cagataggca agaaggcgac
     6181 gacgctggcc gacgagcttg aggaggccgc gacgcgaatg gttgtctttg gggaagccgc
     6241 ggagatggtc ggtcaggctc cggattcgct ccgtcaggag cgcgatctgg acctccggcg
     6301 acccggtgtc accttccttg gtggcatacc cggtcatgat ctcctgcttc tgttcccgcg
     6361 cgagcggcac tcgttcctcc gaacacgaac ttctatcgtt gtct
//
LOCUS       DATAIW010000448        16560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_194716, whole genome shotgun sequence.
ACCESSION   DATAIW010000448 DATAIW010000000
VERSION     DATAIW010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194716"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VHM48_13390"
     CDS             complement(<1..773)
                     /locus_tag="VHM48_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756456.1"
                     /translation="MNPALAALAAVTIAGAVLAVTARDVRATVLGLLVVLLGAPLIAD
                     PWPDPLAILARIAAALLAIRLIAIGVRGELSTTGSRVGWPTEALLAAAAAVVGFGSHG
                     LGAPGLGAPEAQAAGFALLVLAAAPLITGRDVLRLAVGALLLLVGASSIRAALDSPPN
                     QAEQLVDAVLTIAIGGAIAVIATAARAAGGLTAIDAGGPGAAARHMPDAHRPTDRPSR
                     AAAPDRAPAGPKVAPASPKVAPAGPKVVPAGLKGTRRPG"
     gene            complement(799..1395)
                     /gene="lepB"
                     /locus_tag="VHM48_13395"
     CDS             complement(799..1395)
                     /gene="lepB"
                     /locus_tag="VHM48_13395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2756457.1"
                     /translation="MTRKPALGCLLEIVETLVLTLIIFWVIQSFVAQPFRVQQKSMQH
                     TLEESQYVLVDKLTPNFDTYKRGDIVVFTPPPNWAAPGGEPYIKRAIGVGGDTVQVRD
                     GSVFLNGTKLVEPYVFKEDDGQTQATEPLTSVDNWVIPDGQLFLMGDHRGNSADSREF
                     GPIDIKVVIGRAWLRYWPIDTFTILQTSSHPELATPAP"
     gene            complement(1540..2253)
                     /gene="lepB"
                     /locus_tag="VHM48_13400"
     CDS             complement(1540..2253)
                     /gene="lepB"
                     /locus_tag="VHM48_13400"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2756458.1"
                     /translation="MTIPPPEPAPTPTNRRALGCLFEIIETVVLTLILFWVIQSFVAQ
                     PFQVQQFSMQNTIQDGQYVLVDRLTPHFDPYHRGDIIVFTPPQAVDESPDRKPFIKRV
                     IGVAGDTIELRDGKVLVNGVALVEPYLYAVNGTAQATGPENGEVTWTVGAGELLVMGD
                     HRARSSDSREFGTIEVSSVIGRAWLRYWPIEDLAVLPTAQHPELAKPSPGPSVGPSSA
                     PSARPSAKPTKRASASPKR"
     gene            2550..4757
                     /locus_tag="VHM48_13405"
     CDS             2550..4757
                     /locus_tag="VHM48_13405"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX2756459.1"
                     /translation="MSADTLQLIILPAGIIAILFALYLARDVLSRDTGTKEMEDVAGT
                     IFEGAVAFIRRQYTTIGILALVGSVVIGGVITIFETKQVADTKTFGLDLGWRTALAFL
                     VGAACSMASGIIGMFISVKANVRTAAAARRSLVEAVQVAMRGGAVSGFLVVALSLLGV
                     YGIFSLFGGLGGGQATQDAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVGKV
                     EKGIPEDDPRNAGVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGVYLIALKS
                     GWPHPEAWIFFPLVVRAFGLLSTIVAIFFVRGREDENPMNMLNRGYWVTTILSAAALF
                     LVTNVMMQTNGNAPSGLPVWLYYFAAGVVGLATSVAFVYITQYYTAGTFRPVREIAEA
                     SRTGPATNIISGMAVGFETTFVTAITIGIALFASHWLGSEAGIRNADGKDVGGIFGTA
                     VATMGMLMTTAYILAMDTFGPITDNAGGIAEFSRAEAGAREITDRLDAVGNTTKALTK
                     GYAIASASLAAFLLFSAYIDKVNLIHTNRGEALLTSVNLANVNVFIAGLMGAMLVYFF
                     SSLAIRAVGTTAQAIIVEVRRQFREMPGIMDYTQRPDYARVVDITTRAALREMILPGA
                     VAVATPIIVGLLLGAEAVAGMLMVGTIAGVLLATVLNNGGGAWDNAKKYIESGNLKDD
                     AGNVLGKKTDAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLFIR"
     gene            4864..5682
                     /locus_tag="VHM48_13410"
     CDS             4864..5682
                     /locus_tag="VHM48_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2756460.1"
                     /translation="MDTLLAALIMGIVQGLTEFLPVSSSGHLIVVPYLLGIKDPFITS
                     LEFSVMLHIGTLLALLLYFRTDWLRLVPAFFAILRDRSLDGDPDRRLAALLAVATLPA
                     LVLGFLLHDLEDVIREVGPVAVTLVIGAAILWLAERTGRRRLVALDLSFAQALAIGGA
                     QAIALIPGISRSGISISAGLFAGLRREEAARFSFLMATLITAAAAAYELLTVIRGGGV
                     PVQVAPLAVGLVASFVFGVLAIAVLLRFLRTQSTDIFVAYRVVLAAIVLILWLG"
     gene            5740..6204
                     /gene="argR"
                     /locus_tag="VHM48_13415"
     CDS             5740..6204
                     /gene="argR"
                     /locus_tag="VHM48_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX2756461.1"
                     /translation="MEVMKQLRHRAIRDLVSTRPIRTQQELAAVLRERGFRTTQATMS
                     RDVAELGLIKAARDGTQAYSLPKRLIDAETSGEDRLRALLHDLPIEIREAGLLLVIRT
                     LPGSAHAIAAALDRARWPEVVGSIAGDDTVFVACPDRTALRRATARVREYAG"
     gene            complement(6260..6598)
                     /locus_tag="VHM48_13420"
     CDS             complement(6260..6598)
                     /locus_tag="VHM48_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756462.1"
                     /translation="MLDLASPFETQALHHRAGSPVRLVGRREHLAIRESIQRVPHERG
                     SDAPSAEALRHNDEPDERLLEEAGIDDRVPENFTFRFRHPALAKDQGIRDALSAIRVS
                     RPGTDGNDAR"
     gene            6827..7291
                     /gene="greA"
                     /locus_tag="VHM48_13425"
     CDS             6827..7291
                     /gene="greA"
                     /locus_tag="VHM48_13425"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2756463.1"
                     /translation="MNMKPTYLSKEGLDKLRAELDEMTSVKRPEVAQRIHDAKEHGDL
                     SENAEYEDAKNEQAFVEGRIQTLQALIKNATIIDEHHSTDHVQIGSTVKVASDDGEES
                     FTIVGSTEARPTEGRISNESPVGRALLGKKKGEKVVVKVPAGDFTYKVVNIT"
     gene            7314..8999
                     /gene="lysS"
                     /locus_tag="VHM48_13430"
     CDS             7314..8999
                     /gene="lysS"
                     /locus_tag="VHM48_13430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX2756464.1"
                     /translation="MTGQSRSEAGGRAPRGHPPEDWADELAASVSGPQVVNDSKTPSG
                     TVHVGSLRGPVILDTIWRALRARGVEAHLLYGVDDLDPMDAQALLTPDAIDREMGRPL
                     AHVPDPAGDCHASYARHFAGIFIETFRRLGIVPERYYWMSDIYPTGQMDGFIRTALDR
                     ASVVRDIYRRVANVQHPDAWHPINVICENCGKIGTTIVTKWDGERVFYDCRETLVTWA
                     RGCGNSGWVAPFGGRAKLPWNLEWAAQWSLLGVTIEPCGKDLSTAGGSRDRSDAIARE
                     VFEREPPLNVPYEFINIGGKKMSTSKGRGAAAHEIVEVIPPEQLRFFFLRPKPNSAVD
                     FEPNQTDAVPRLFDEFDRFAAATAGKEVRGEIAPGFEGTFAYSLLDPDADVALEAAAF
                     RPAFSHLALLLQVPNVDVAARVAAEKGSALTDREAATFDERVRAARAWLDAYAPERAI
                     VAIREELPRDAVAALDGDQRRYLGALSDAALSNDTLPASGEAWQDLIFTVAGEAGLPN
                     GRAFAALYAAFLGRSNGPRAGWLLASLDGGFVTARLRASASPDGAPAGVGGAA"
     gene            8996..10342
                     /gene="serS"
                     /locus_tag="VHM48_13435"
     CDS             8996..10342
                     /gene="serS"
                     /locus_tag="VHM48_13435"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2756465.1"
                     /translation="MSVGLQRLREDAETVRRGAIDKGEDPATVDRALELDARRRTLLG
                     EGDALKAERNATSKEVGEAIKGGARPDGPGVVALKAASVEASRKIEAIDAELASVEAE
                     VEQLLLRIPNPADPDVPVGGEEANVTVRTWGDLLPVDQPVVGEVGADTPVGGATWRRR
                     PHWEIATELDIIDNPRGAKIAGSGFPVYKGAGSALQRALINFFLDVHTRENGMTEIWP
                     PAVVNAASAIGTGQIPDKEDQMYVVTRDELYLVPTAEVPVTNLHRDEILEADQLPIRY
                     AAYSPCFRREAGAAGKDTRGILRVHQFDKVEMVFFEKPADSAAALEWLTERAEILLQR
                     LGLAYRVLLMATRDMGFVQAKKYDLEVWSPGVEKWLEVSSCSNFRDYQARRMAIRWRP
                     EPGAKPELVHTLNGSGLALARVVAAILETYQRPDGTVEVPEVLRSYLGRDAIAARD"
     gene            10347..11159
                     /locus_tag="VHM48_13440"
     CDS             10347..11159
                     /locus_tag="VHM48_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756466.1"
                     /translation="MTGPTGGPGTVEARLRAHGLALPPGHTLTTLTERPDLDRAVDAH
                     HAGLWEPFMLESEVANRMFPRAHADWPDHQLVLLDAAGAVVATSNAMPLAWDGTDADL
                     PDSWDEQVLRSAADVDAGRAPTALGAMLIVVGRTLRGGGLSGVVLGAMRAAGRAAGYR
                     AVIACVRPTEKHRYPLTPIERYAAWTRPDGLPFDPWIRLHVRLGGRIVRPAPRSMTIR
                     GSVADWEGWTGLAFPDSGDYVIKQATSPVAIDRERDEGVYFDQNVWIVHDLA"
     gene            11271..11669
                     /locus_tag="VHM48_13445"
     CDS             11271..11669
                     /locus_tag="VHM48_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2756467.1"
                     /translation="MTMTQKVFEPFSADIAARAKANPYFREVVSTGPHAQVVLMSIPA
                     GGDIGEEVHPDVDQVLVFVDGEGVAVLEGQRSPVRAGRLVHVPAGTRHNFVNEGSVDL
                     KLYTVYAPPQHAPGTVHRTKAEADADDEHA"
     gene            11875..12876
                     /locus_tag="VHM48_13450"
     CDS             11875..12876
                     /locus_tag="VHM48_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX2756468.1"
                     /translation="MINVLILLAGAVILGLSLFIGVVLPLGRQVPAGRNAPPLPRAHI
                     ARGVLGAAIGALILVAGLSQPFVEVPAGNVGVVTNFGSVQTGTLEPGLHIVIPIVQHV
                     AIIDTRVQPHQFQEIDAASAEYQTVKLTGVMNYHVDGQFASDLYQRVGTDFASKVIDP
                     AFNDFIKTVVPAYKVDAILGARDEIRSKAKEALAANLAQYHIVVDDIYIANIAFSDAF
                     QQAIEAKQVAQQQVQTEQQILAQKKIQADQAVAQAQGQADSNVKLAEGQAAATIALAN
                     GQATANKALAASLSDQILQYQYIQKLADKITVMLVPSGNASIFDLKGLLNPQASAAP"
     gene            13076..13810
                     /locus_tag="VHM48_13455"
     CDS             13076..13810
                     /locus_tag="VHM48_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2756469.1"
                     /translation="MSAVVADDLRQSIEKRTLGANGRLPSEPELARRMGVSRATIRQA
                     VTELEEAGFVRRRQGRGTFVTGQATSLRNNLNVNSGVTDLIAAGGWTPGTTDVTREMR
                     GATREEAEALGLRRGDAVVVVRRTRLADGRPVVAVEDVLSADRLASASTTPDALVAAL
                     ATQSLYDVLDGLGLAVHHGIADIRPARAGGDVARRLDVTSGSLVVLIEQIDFTVDDEP
                     VLFSRERHRPDVFQFRVYRRGPGSRR"
     gene            13922..15364
                     /locus_tag="VHM48_13460"
     CDS             13922..15364
                     /locus_tag="VHM48_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2756470.1"
                     /translation="MTGLTAPADGAVATPGLRRNAVGLLAAIMLSATIMGPAVSTYFN
                     TQLTAKFAGAALPFVYLVALIAALIVANGVAEMARKLPAAGAFYTYVTRGIGPKAGFI
                     TGTLMFLGYSLLEPAELALIGYWISNVLLRFGYDVSWILIAAIFWVTTFVLSVRGIRI
                     SMETALIAFAIEVGVIIVLCVLILGKGGAEGINLTPFDPGQSPTGVSGIALGLVFGIL
                     SFVGFEGAATLGEEVENPRVNVPRAIFGATLLVGVIYLFATWAETLGFGVSTISVLQN
                     DAAPFDTLAKTYSPDWFRVLVDIAGASSMFGVMISSHNGITRIIYAMGREKLLPAFLG
                     TINGKYSTPQMAIIVQSVGSAIIAIILGLVAGPFNTYAYLGTLLTLGIIPVYILVNVA
                     VMRYFRSQYPTEFNVIKHAILPVAGILLMAIPIYGLVWPIPDYPYNLFPYIVVAYIAI
                     SFAIAVWLERNRPTELREAGKILGAEYDIA"
     gene            15532..>16560
                     /locus_tag="VHM48_13465"
     CDS             15532..>16560
                     /locus_tag="VHM48_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2756471.1"
                     /translation="MGHVIGIDLGSQSLKGLLLDPAGRLIGEASRSYDLAYPFPAWAQ
                     QDPADWWTAIRAVVGELVAAAALAPGDVSALAFASQVDGVVAVDVEGEDLHPAIIWLD
                     RRAEAEAAALGRRISPEALFERSGLNLDSSHVAPKILWLRAHVPDAYARAATLHLPGS
                     WAVARLTGVSVVDHSNASSTLLYDVRTRDWSDELLAAADVERSRLPVVAASTAVAGTL
                     TAHAAAELGLTTACLVAVGSGDEHAACLGAGVLRAGPVCDIAGTAEPVAVASDLPVFD
                     RTGLVETHAHADPRSWLIENPGFVSGGSVRWFNDTIGRILRDVGDRAGPDADDAAGAL
                     DRGIGLGQG"
BASE COUNT         2448 a         5921 c         5640 g         2551 t
ORIGIN      
        1 tcgcccgggc gccgggtacc cttcaggccc gccgggacga ccttcgggcc cgcaggcgcg
       61 accttcgggc tcgccggggc caccttcggg cccgccggag cccgatccgg agcggccgct
      121 cgcgacggac gatccgtcgg ccgatgggcg tcgggcatgt ggcgcgcggc cgcgccgggc
      181 ccgccggcat cgatcgcggt gagccctcct gcggcgcggg ccgcggtggc gatcacggcg
      241 atcgcgccgc cgatggcgat cgtcaggacc gcatccacga gctgctcggc ctggttcggc
      301 ggcgaatcga gcgcggcccg gatcgacgac gcgccgacga ggagcaggag cgcgccgacg
      361 gccaggcgga ggacgtcccg gccggtgatc aggggagcgg ccgcgagcac gaggagggcg
      421 aaccccgctg cctgggcctc gggcgccccg agccccgggg caccgagacc gtggcttccg
      481 aacccgacga ccgccgcggc ggccgccagg agcgcctcgg tcggccagcc gacccggctc
      541 ccggtcgtgc tcagctcgcc gcggacgccg atggcgatga ggcggatcgc gagcagcgcg
      601 gcggcgatcc gggcgaggat cgcgagggga tcgggccacg gatcggcaat gagcggcgcc
      661 ccgagcagga cgacgagcag gccgagaacg gtcgcccgga cgtcgcgggc cgtgaccgcc
      721 aggacggctc ccgcgatcgt gaccgcggcg agcgcggcga gggccgggtt cacgccgcgt
      781 gtcgccggtc gcggccgctc atggggccgg ggtcgcgagc tccgggtggc tggaggtctg
      841 gaggatcgtg aaggtatcga tcggccagta gcggagccac gcccgcccga tgacgacctt
      901 gatgtcgatc ggtccgaact cgcgcgagtc cgccgagttc ccgcgatggt cgcccatcag
      961 gaagagctgt ccatcgggga tgacccagtt gtcgacgctg gtcagcggct ccgtggcctg
     1021 cgtctggccg tcgtcctcct tgaagacata cggttcgacg agcttcgtgc cgttcaggaa
     1081 gaccgagccg tcgcggacct ggacggtgtc gccgccgacg ccgatcgccc gcttgatgta
     1141 cggctcgccg ccgggtgcgg cccagttcgg tggtggcgtg aatacgacga tgtcgccgcg
     1201 cttgtaggtg tcgaagttgg gggtcagctt gtcgacgagg acgtactgac tctcctccag
     1261 cgtgtgctgc atcgacttct gctggacccg gaacggttgg gcgacgaagg attggatgac
     1321 ccagaagatg atcagggtca ggacgagggt ctcgacgatc tccaggagac acccgagcgc
     1381 gggcttccgg gtcacgaggt cgggatcggg gtggtcacaa gtccggcagt ataggtcgtc
     1441 aggccgacgg gccccgatcg accgaggtgg cccgatcgcc gtctcggcgt ggcggatcgt
     1501 cgggcgccgc gagacaggcg tggaagcccg ctggcgggct cagcgcttgg gactggcgga
     1561 cgctcgcttc gtcggcttcg ccgatggcct ggccgacggc gccgacgacg gcccgaccga
     1621 cggccccggc gacggcttgg ccagctcggg gtgctgggcg gtcgggagca cggcgaggtc
     1681 ctcgatcggc cagtagcgga gccacgcccg gccgatcacc gagctgacct cgatcgtgcc
     1741 gaactcgcgc gagtccgacg agcgggcgcg atgatcgccc atgaccagca gctcgccggc
     1801 gccgaccgtc caggtgacct cgccgttctc gggccccgtg gcctgggcgg tgccgttgac
     1861 ggcatacaga tatggctcga cgagggcgac accgttgacg aggaccttgc cgtcgcggag
     1921 ctcgatcgtg tcgccggcaa cgccgatcac ccgcttgatg aagggcttcc gatcgggcga
     1981 ttcgtcgacc gcctgcggcg gcgtgaacac gatgatgtcg ccgcggtgat acgggtcgaa
     2041 atggggggtc agccggtcga cgaggacgta ctggccgtcc tggatcgtgt tctgcatcga
     2101 gaactgctgg acctggaagg gctgggccac gaacgactgg atcacccaga acaggatcag
     2161 cgtcaggacg accgtctcga tgatctcgaa gaggcagccg agggcacgtc ggttggtcgg
     2221 agtcggagcc ggctccggag gagggatggt cacagcggtg aagcataggg tgccgacgcc
     2281 gtttcgacgt cgggaccatg gaggccggcc gccagggacc ttcggcgccc tccatcaccc
     2341 gaccccgtgg tgatccacac ggccggccgg gcgtggcgcg gtgtggcggc tgtgcggtgt
     2401 ggcggctgtg ctcccggctt tgctacactc ccacgcggcc cgtccgccga catcgcacgc
     2461 gggctgcccc gatccccgga ccgacgcgct ggttccgctg cgcgatccgc gaccggtgcc
     2521 ccagagagag tggaggaccc cgaaccccga tgagcgcaga cacgctccag ttgatcatcc
     2581 tgcccgcggg catcatcgcc atcctgttcg ccctctacct ggctcgtgac gtcctgtcgc
     2641 gtgacaccgg gacgaaggag atggaagacg tcgccgggac gatcttcgag ggcgcggtcg
     2701 ccttcatccg gcgccagtac acgacgatcg gcatcctcgc tctcgtcggg tcggtcgtca
     2761 tcggcggggt catcacgatc ttcgagacga agcaggtcgc cgacacgaag accttcggcc
     2821 tcgacctcgg ctggcggacc gccctcgcct tcctcgtggg cgcggcgtgc tcgatggcgt
     2881 cggggatcat cggcatgttc atcagcgtga aggcgaacgt ccgcacggcg gcggcggccc
     2941 ggcgcagcct cgtcgaggcg gtccaggtcg cgatgcgcgg cggcgcggtg tcgggcttcc
     3001 tcgtcgtcgc cctctccctc ctcggcgtgt acggcatctt cagcctgttc ggcgggctgg
     3061 gcggcggcca ggcgacgcag gacgcgccgt tcctgatcgt cggcttcggc ttcggcgcct
     3121 ccttcgtcgc cctgttcgcc caactcggcg gcgggatcta cacgaaggcc gccgacgtcg
     3181 gctccgacct cgtcggcaag gtcgagaagg ggatcccgga ggacgacccg cggaacgccg
     3241 gtgtcatcgc cgacctcgtc ggcgacaacg tcggcgattg cgccggtcgt ggcgccgacc
     3301 tcttcgaatc cacggcggcc gagaacatcg gggcgatgat cctcggcgtc ggggtctatc
     3361 tgatcgccct caagtcgggc tggccgcatc cggaggcctg gatcttcttc ccgctcgtcg
     3421 tccgggcatt cggtctcctg tcgacgatcg tggcgatctt cttcgtccgc ggtcgcgagg
     3481 acgagaaccc gatgaacatg ctgaaccgcg gctactgggt cacgacgatc ctgtccgccg
     3541 ctgccctctt tctggtcacg aacgtgatga tgcagacgaa cggcaatgcg ccgtccggat
     3601 tgccggtctg gctgtactac ttcgcggccg gcgtcgtcgg gcttgcgacg agcgtcgcgt
     3661 tcgtctacat cacccagtac tacacggccg gcacgtttcg cccggtccgc gagatcgcgg
     3721 aggcgtcccg aacgggcccc gcgacgaaca tcatctccgg gatggccgtc ggcttcgaga
     3781 cgaccttcgt caccgcgatc accatcggca tcgccctctt cgccagccac tggctcggct
     3841 ccgaggccgg catcaggaac gccgacggca aggacgtcgg cgggatcttc gggacggcgg
     3901 tcgcgacgat ggggatgctc atgaccaccg cctacatcct cgcgatggac acgttcggcc
     3961 cgatcaccga caacgccggc ggcatcgccg agttcagccg ggccgaggcc ggcgcccgcg
     4021 agatcaccga ccggctcgac gcggtcggca acacgaccaa ggccctgacg aagggctacg
     4081 cgatcgcgtc ggcgtcgctc gccgcgttcc tgctgttcag cgcctacatc gacaaggtca
     4141 acctgatcca cacgaaccgc ggtgaggccc ttctgacctc ggtcaacctc gccaacgtca
     4201 atgtcttcat cgccggcctg atgggcgcga tgctggtgta cttcttctcg tcgctcgcca
     4261 tccgggccgt cggcacgacg gctcaggcga tcatcgtcga ggtccgccgc cagttccgcg
     4321 aaatgcccgg gatcatggat tacacccagc gcccggacta cgcccgggtg gtggacatca
     4381 cgacccgcgc cgcgctccgc gagatgatcc tgccgggcgc ggtggccgtc gcgaccccga
     4441 tcatcgtggg gcttcttctc ggagccgaag cggtggccgg catgctgatg gtcggcacga
     4501 tcgccggcgt cctgctcgcg accgtcctca acaacggcgg cggggcatgg gacaacgcga
     4561 agaagtacat cgagtcgggc aacctcaagg acgatgccgg caatgtcctc ggcaagaaga
     4621 ccgacgccca cgcggcggcc gttgtcggcg acacggtcgg cgacccgttc aaggacaccg
     4681 ccggtccgtc gctccacgtc ctcgtcaagc tcctggccac gatcaccctc gtcctggcac
     4741 cgctcttcat tcgctagtct cgccggcggg cggcgtcacg cgggtcctcg acccgcaccg
     4801 gcgtcgcctg tcgcgatcca actccccggt cagcccagtc cggcccagtc cgaacggaca
     4861 tcgatggaca ctcttctcgc ggccttgatc atgggcatcg tccaggggct gaccgagttc
     4921 ctgcccgtgt cctccagcgg gcacctgatc gtggttccgt acctcctcgg catcaaggat
     4981 ccgttcatca catcgctcga gttcagcgtc atgctccaca tcggaacgct gctcgcgctg
     5041 ctgctctact tccggaccga ttggctccgg ctcgtcccgg cgttcttcgc catcctccgc
     5101 gaccgctcgc tcgacggcga tccggaccgg cgcctggccg cgcttctcgc cgtcgccacg
     5161 ctgccggcgc tggtgctcgg cttcctcctc cacgatctcg aggacgtgat ccgcgaggtc
     5221 ggcccggttg cggtgaccct cgtcatcggt gcggcgatcc tgtggctggc cgagcggacc
     5281 ggccggcgcc ggctcgtggc tctcgacctg tcgttcgccc aggcgctcgc gattggcggc
     5341 gcccaggcca tcgccctgat ccccggcatc agccgatcgg gaatctcgat ctcggccggg
     5401 ctgttcgccg ggctccggcg cgaggaggcg gcccgcttca gcttcctgat ggcgactctg
     5461 atcacggccg ccgccgccgc gtacgagctg ttgaccgtga tccgcggcgg tggggtgccg
     5521 gtccaggtcg cgccgctcgc ggtcggcctc gtcgcctcgt tcgtgttcgg cgtgctcgcg
     5581 atcgcggtcc tgctccggtt cctgcggacc cagtcgaccg acatcttcgt ggcctaccgg
     5641 gtggtcctcg ccgcgatcgt cctgatcctc tggctcggct gagccgggtc cgaacccgcg
     5701 acggtcaaac cgactgtcga ggcagtaccc tccagtccga tggaagtgat gaagcagctc
     5761 cgccatcggg cgatccgcga cctggtctcg acgcgaccga tccgtaccca gcaggagctc
     5821 gccgccgtcc tccgcgagcg cggcttcagg acgacccagg cgacgatgtc gcgcgacgtc
     5881 gccgagctcg gcctcatcaa ggcggcccgc gacggaacgc aggcctactc cctgccgaag
     5941 cggctcatcg atgcggagac ctccggcgag gaccgcctcc gtgccctgct tcacgacctg
     6001 ccgatcgaga tccgcgaggc gggcctgctg ctcgtgatcc ggacgctgcc gggctcggcc
     6061 catgccatcg ccgccgcgct ggaccgggcg cgctggccgg aggtcgtcgg atcgattgcg
     6121 ggcgacgaca cggtgttcgt ggcctgcccg gatcggacgg ccctgcgccg ggcaacggcc
     6181 cgcgtccgcg agtacgcggg ctgacggaat gcccgcacaa gtcgatcgcc tcgagatccg
     6241 gctggcggcg ctcggcgatc tacctcgcgt cgtttccatc ggtacccggg cgactgaccc
     6301 ggatcgccga caacgcgtcg cggatgccct ggtccttggc gagtgctggg tggcggaatc
     6361 ggaaggtgaa gttctcgggt acgcggtcgt caataccggc ttcttcgagc aaccgttcgt
     6421 ctggctcgtc gttgtggcgg agcgcttccg ccgacggggc gtcggatccg cgctcatgcg
     6481 gcacacgctg gatcgattcc cggatcgcaa ggtgttcacg tcgaccaacc agtcgaaccg
     6541 ggctgcccgc gcgatgatgg agagcctggg tttcgaacgg gctggccaga tcgagcacct
     6601 ggacgaagga gaccccgagc tcgtttacgt gaggcgaccc ggcccaacct gagacaccac
     6661 ggagcgacca gttcctttga caccgatgac cccccgcgct accctttcgg ccggtcgaac
     6721 ttcaggcgcg acgagtggcg cgcagcctgg agttctttcc tttgtctgaa ccccgtggac
     6781 gcggccgtcc ggcccgtcga gccagcatct gggggcgccc gacccgatga acatgaagcc
     6841 gacctacctc tcgaaggagg gcctcgacaa gctccgcgcg gagctcgacg agatgacgtc
     6901 cgtcaagcgg ccggaggtcg cccagcggat ccacgatgcc aaggagcacg gcgacctcag
     6961 cgagaacgcc gagtacgagg acgccaagaa cgagcaggcc ttcgtcgagg gccggatcca
     7021 gacgctccag gcgctgatca agaacgcgac gatcatcgac gagcaccact cgaccgacca
     7081 tgtccagatc ggctcgaccg tcaaggtcgc cagcgacgac ggcgaggagt cgttcacgat
     7141 cgtcggctcg accgaggccc gaccgacgga gggccggatc agcaacgaga gccccgtcgg
     7201 gcgcgccctt ctcggcaaga agaagggcga gaaggtcgtc gtgaaggttc ccgccggcga
     7261 cttcacctac aaggtcgtca acatcacgta agcgggaggt ggcaacctcc accatgactg
     7321 ggcaatcgcg ctccgaggcc ggaggccgag ctccgcgagg ccatccgccc gaggattggg
     7381 ccgacgaact ggccgccagc gtcagcggac cgcaggtcgt caacgacagc aagacgccgt
     7441 cggggacggt ccacgtcggc tcgctccgcg gcccggtcat cctcgacacg atctggcggg
     7501 cgctgcgtgc ccgcggcgtc gaggcccacc tcctgtacgg cgtcgacgac ctcgacccga
     7561 tggacgccca ggcgctcctg acgcccgacg cgatcgaccg cgagatgggt cggcccctgg
     7621 cccacgtccc ggatccggcc ggcgattgcc acgcgagcta cgcccgccat ttcgccggga
     7681 tcttcatcga gacgttccgc cgcctcggga tcgtcccgga gcggtactac tggatgagcg
     7741 acatctaccc taccgggcag atggacggtt tcatccggac cgcgctcgac cgggcgtcgg
     7801 tcgtccgcga catctaccga cgggtcgcca acgtccagca ccccgatgcc tggcacccga
     7861 tcaacgtcat ctgcgagaac tgcggcaaga tcgggacgac aatcgtcacc aagtgggacg
     7921 gcgagcgggt cttctacgat tgccgcgaga cgctcgtgac gtgggcgcgc ggttgcggca
     7981 attccggttg ggtcgcgccg ttcggcgggc gggccaagct tccctggaac ctcgaatggg
     8041 cggcccaatg gtcgctcctc ggggtgacga tcgagccgtg cggcaaggac ctctcgaccg
     8101 cgggcggatc ccgcgaccgg agcgacgcga tcgcccgcga agtcttcgag cgcgagccgc
     8161 cgctcaacgt cccgtacgag ttcatcaaca tcggcggcaa gaagatgtcg acctcgaagg
     8221 gccgcggcgc ggcggcccac gagatcgtcg aggtcatccc gcccgaacag ctccgcttct
     8281 tcttcctccg tccgaagccc aacagcgcgg tcgacttcga gccgaaccag accgacgcgg
     8341 tgccgcgcct gttcgacgag ttcgaccggt tcgccgccgc caccgccggc aaggaggtcc
     8401 gtggcgagat cgctcccggg ttcgaaggca cgttcgccta ttcgctgctc gacccggacg
     8461 cggacgtcgc cctcgaggcg gcggccttcc ggccggcctt ctcgcacctc gcgctcctgc
     8521 tccaggtccc gaacgtggac gtcgccgccc gggtcgcggc agagaagggg agcgcgctga
     8581 ccgatcgtga ggccgcgacg ttcgacgagc gcgtccgcgc ggcccgggcc tggctggatg
     8641 cctacgcccc cgagcgggcg atcgtcgcga tccgcgagga gctcccgagg gacgcggtcg
     8701 cggccctcga cggcgaccag cggcgctacc tcggcgcgct ctccgacgcc gctctatcga
     8761 acgacacgct gcccgcgagc ggagaggcct ggcaggacct gatcttcacc gtcgccggtg
     8821 aggccggcct gccgaacggc cgcgccttcg cggcgctcta cgccgcgttc ctcggccgat
     8881 ccaacgggcc tcgagccggc tggctcctgg cgtcgctgga cggcgggttc gtgaccgccc
     8941 gattgcgggc atcggcatcc ccggatggcg ccccggcggg cgtcggaggt gcggcatgag
     9001 cgtcgggctg caacggctgc gtgaggacgc ggagaccgtt cgccgcgggg cgatcgacaa
     9061 gggcgaggat ccggcgaccg tcgacagggc gctcgagctg gatgcccggc ggcggaccct
     9121 cctcggcgag ggcgacgcgc tcaaggctga gcgcaacgcg acaagcaagg aggtcggcga
     9181 ggccatcaag gggggagcca ggccggacgg ccccggggtc gtggcgctca aggccgcgtc
     9241 ggtcgaggcc agccggaaga tcgaggcgat cgacgcggag ctcgcgtcgg tcgaggccga
     9301 ggtcgagcag ctcctcctcc ggatcccgaa ccccgccgat cccgacgtgc ctgtcggcgg
     9361 cgaggaggcg aacgtcacgg tccgcacctg gggcgacctg ctgccggtcg accagccggt
     9421 cgtcggcgag gtcggcgcgg acacgcccgt cggcggcgcg acgtggcggc ggcgaccgca
     9481 ctgggagatc gcgaccgagc tcgacatcat cgacaacccc cgtggtgcga agatcgccgg
     9541 ctccgggttc ccggtctaca agggcgcggg ctccgcgctc cagcgcgccc tgatcaactt
     9601 cttcctcgat gtccataccc gcgagaacgg gatgaccgag atctggccgc cggccgtcgt
     9661 caacgccgcg tcggcgatcg gcaccgggca gatcccggac aaggaagacc agatgtacgt
     9721 cgtcacccgc gacgagctgt acctggtccc gacggccgag gtcccggtca cgaacctcca
     9781 ccgtgacgag atcctcgagg ccgatcagct cccgatccgc tatgcggcct actcgccctg
     9841 cttccggcgc gaggccggcg ccgccggcaa ggacacccgt gggatcctcc gggtccacca
     9901 gttcgacaag gtcgagatgg tcttcttcga gaagccggcc gactcggcag ccgcgctcga
     9961 atggctgacc gagcgggccg agatcctcct ccagcggctc ggcctggcct accgcgtgct
    10021 cctcatggcg acccgcgaca tgggcttcgt ccaggcgaag aagtacgacc tcgaggtctg
    10081 gtcgcccggc gtcgagaagt ggctcgaggt cagctcctgc tcgaacttcc gcgactacca
    10141 ggcacggcgg atggcaatcc gctggcggcc ggagcccggc gcgaagccgg agctcgtcca
    10201 caccctcaac ggctcgggcc tggccctggc ccgggtggtc gcggcgatcc tcgagaccta
    10261 ccagcgcccc gacgggaccg tcgaggtgcc cgaggtcctt cggtcctacc tcgggcgcga
    10321 cgcgatcgcc gcccgggatt gacgccgtga ccgggccgac cggcgggccc gggaccgtcg
    10381 aggcgcgcct ccgggcccac ggtctggcgc tgccgcccgg ccacacgctg acgaccctca
    10441 cggagcgtcc ggacctcgac cgggcggtcg atgcccacca cgccggcctg tgggagccgt
    10501 tcatgctcga gagcgaggtg gcgaaccgga tgttcccccg cgcccacgcc gactggcccg
    10561 accaccagct ggtcctgctc gacgccgccg gcgcggtcgt ggcgacctcg aacgcgatgc
    10621 ccctcgcctg ggatggcacg gacgccgacc tccccgacag ctgggacgag caggtcctcc
    10681 ggagcgcagc cgacgtcgac gccggccggg cgccgaccgc cctcggcgcg atgctgatcg
    10741 tcgtcgggcg cacccttcgc gggggcgggc tgtcgggggt cgtgctcggc gcgatgcggg
    10801 ccgccggccg ggcagcgggc taccgggcgg tcatcgcctg cgtccggccg accgagaagc
    10861 atcgctaccc gctcaccccg atcgagcgct atgcggcgtg gacccgtccc gacggcctgc
    10921 cgttcgaccc gtggatccga ctccacgtgc ggctcggtgg ccggatcgtc cgccccgccc
    10981 cgcgctcgat gacgatccgc ggctcggtgg cagactggga aggctggacc gggctcgcct
    11041 ttccggacag tggcgactac gtgatcaagc aggcgacgtc gccggtcgcg atcgaccggg
    11101 agcgcgacga gggcgtctac ttcgaccaga acgtctggat cgtccacgac ctcgcctgat
    11161 ccttcgccgg gacctgacct cgatcgtggc agccctgccg tttgacccgc cgttcggctc
    11221 tggtggcctc gacgagatcg ggccatcgtt ggggtgaaag agaggtcgcc atgaccatga
    11281 cccagaaggt cttcgagccg ttcagcgcgg acatcgccgc gcgggcgaag gccaacccct
    11341 acttccgcga ggtcgtctcg accgggccgc acgcccaggt cgtcctgatg agcatcccgg
    11401 cgggcggcga catcggcgag gaggtccacc cggacgtcga ccaggtcctc gtgttcgtcg
    11461 atggcgaggg cgtggccgtc ctcgaaggcc agcggagccc ggtgcgggcc ggccgcctcg
    11521 tccacgtccc cgcgggcacg cgccacaact tcgtcaacga aggctcggtc gacctgaagc
    11581 tctacacggt ctatgcgccg ccccagcacg cgcccggaac ggtccatcgg accaaggccg
    11641 aggccgatgc cgacgatgag cacgcctgat cgaccgctac tctcgacgtc cccacgctgc
    11701 gacgcccgcc ctcggcgggc gtcgcttgcg tcatccagct gcgggatccg gcatccaatc
    11761 gtccgtggga gacggtcaac ctgaggccga tcgacctgcg gcctgacggc tatcctcgga
    11821 tgtgagcccg atcccggccg tcccagccgt ccttgttgag aggtccccgt cacgatgatc
    11881 aatgtcctga tcctgctcgc cggtgccgtc atcctcgggc tgagcctgtt catcggggtc
    11941 gtcctgccgc tcggccgcca ggtaccggcc ggccggaacg cgccgccgct cccgcgagcc
    12001 cacatcgcgc gcggcgtcct cggcgcggcg atcggcgccc tgatcctcgt cgccgggctg
    12061 tcgcagccat tcgtcgaggt cccggccggc aacgtcgggg tcgtcaccaa cttcggatcc
    12121 gtccagacgg gcacgctgga gcccggtctg cacatcgtca tcccgatcgt ccagcacgtc
    12181 gcgatcatcg acacgcgggt ccagccgcat cagttccagg agatcgacgc ggcctccgcc
    12241 gagtaccaga cggtcaagct gaccggcgtg atgaactacc acgtcgacgg ccagttcgcc
    12301 tcggatctct accagcgggt cgggaccgac ttcgcctcga aggtcatcga tcccgcgttc
    12361 aacgacttca tcaagaccgt cgttccggcg tacaaggtcg acgcgatcct cggcgcccgc
    12421 gacgagatcc gctcgaaggc caaggaggcg ctcgccgcga acctcgccca gtaccacatc
    12481 gtcgtcgatg acatctacat cgccaacatc gccttcagcg atgcgttcca gcaggcgatc
    12541 gaggccaagc aggttgccca gcagcaggtc cagaccgagc agcagatcct ggcccagaag
    12601 aagatccagg ccgaccaggc cgtcgcccag gcccagggcc aggccgactc gaacgtcaag
    12661 ctcgccgagg gtcaggccgc tgcgacgatc gccctggcga atggccaggc gaccgccaac
    12721 aaggcgctcg ccgcatcgct gtccgaccag atcctccagt accagtacat ccagaagctg
    12781 gccgacaaga tcacggtgat gctcgtcccg tcgggcaacg cctcgatctt cgacctgaag
    12841 ggactgctca acccgcaggc gtcggccgct ccgtaggggc gacccggccg gcgacgtcgc
    12901 cgggcggcaa cgcccggcca atgatcgctc gcccccgctg gaacccgcgg gatggttcga
    12961 tcctcgattg gtcaggttgt ctgacaacct gttgacatga cgggttacga tggcggcaat
    13021 cagctcacct gagggccgac ccgccaccgt gccaccgctg cagcacccac cgaacttgag
    13081 cgctgtcgtc gccgatgacc ttcgtcagtc gatcgagaag cgcacgctcg gggccaacgg
    13141 ccgcctcccg tccgagccgg agctggcccg ccggatgggc gtgagccggg ctacgatccg
    13201 gcaggccgtg accgagctcg aggaagccgg attcgtccgg cgtcgacagg gccgcggcac
    13261 gttcgtcacc ggccaggcca cctccctgcg caacaacctc aatgtcaatt cgggtgtcac
    13321 ggacctgatc gccgcgggcg gctggacgcc aggaacgacc gacgtgacgc gggagatgcg
    13381 aggcgcgacc cgcgaggagg cggaggccct cggactcagg cgcggcgatg ccgtcgtcgt
    13441 cgtccgccga acgcgactcg ccgacggtcg tccggtcgtc gccgtcgagg acgtgttgag
    13501 cgcggatcgc ctggcctcgg ccagcaccac gcccgacgcc ctggtcgccg ccctcgcgac
    13561 gcagtcgctc tacgacgtgc tcgatggcct cggccttgcc gtccaccacg ggatcgccga
    13621 catccgaccg gcccgcgccg gcggcgacgt cgcccgccga ctggacgtga cgtcggggtc
    13681 gctcgtcgtg ctcatcgaac agatcgactt cacggtcgat gacgagccgg tcctgttctc
    13741 gcgagagcgg caccggccgg acgtgttcca gttccgcgtc tatcggaggg gccccggaag
    13801 ccggcgatga ccccggggtt tggcacccct tatgacccgg cggctgccgg catccgagcg
    13861 ttccattggc gtcacgggcc ggacccggct tcggacgcac ggttgggagg aggaggatca
    13921 catgactgga ttgacggcac cggccgacgg cgcggttgcc acaccgggtc ttcgcaggaa
    13981 tgctgtcggg ctgctggcag cgatcatgct gtcggccacg atcatgggcc cggcggtctc
    14041 gacgtacttc aacacccagt tgacggccaa gttcgcaggc gcggcgctgc cgttcgtcta
    14101 cctcgtcgcg ctgatcgcgg ccctgatcgt cgcgaacggc gtggccgaga tggcccgcaa
    14161 gctgcctgcg gccggcgcgt tctacaccta tgtgacccgc ggcatcggtc cgaaggccgg
    14221 gttcatcacc ggcaccctga tgttcctcgg ctactcgctg ctcgagccgg ccgagctggc
    14281 gctgatcggc tactggatca gcaacgtcct gctccgcttc ggctacgacg tcagctggat
    14341 cctgatcgcg gcgatcttct gggtcacgac attcgtcctg tcggtccgcg gcatccggat
    14401 ctcgatggag accgccctga tcgcgttcgc catcgaggtc ggcgtgatca tcgtgctgtg
    14461 cgtcctgatc ctgggcaagg gcggcgccga gggaattaac ctcacgccgt tcgacccggg
    14521 ccagtcaccc accggggtca gcgggatcgc cctcggtctc gtgttcggca tcctgtcctt
    14581 cgtcggcttc gagggtgcgg ccacgctcgg cgaggaggtc gagaacccgc gggtcaacgt
    14641 gcctcgggcg atcttcgggg cgacgctcct ggtcggcgtg atctacctgt tcgccacctg
    14701 ggccgagacc ctcgggttcg gcgtctcgac gatcagcgtc ctgcagaacg acgccgcgcc
    14761 gttcgacacg ctggcgaaga cctacagccc ggactggttc cgggtcctcg tcgacatcgc
    14821 cggagcctcg agcatgttcg gcgtgatgat ctcgtcccac aacgggatca cccggatcat
    14881 ctacgcgatg ggccgcgaga agctcctgcc ggcgtttctc ggcacgatca acggcaagta
    14941 ctcgacccca cagatggcga tcatcgtcca gtccgtcggg tcggcgatca ttgccatcat
    15001 cctcggcctg gtcgccggtc cgttcaacac gtacgcctat ctcgggacgc tcctgacgct
    15061 cgggatcatc ccggtgtaca tcctcgtgaa cgtcgcggtc atgcgctact tccggagtca
    15121 gtacccgacc gaattcaacg tcatcaagca cgcgatcctg ccggtcgccg gcatcctcct
    15181 gatggcgatc ccgatctacg ggctggtctg gccgatcccg gactatccgt acaacctctt
    15241 cccgtacatc gtcgtcgcgt acatcgcgat cagcttcgcg atcgctgtct ggctcgagcg
    15301 gaaccgtccg accgagctgc gcgaggccgg caagatcctc ggcgccgagt acgacatcgc
    15361 gtagccggtc gtcgagtcct ggtccgcgcc cgccacgtca ccgacgcggc gggcggaccg
    15421 tcacggggac cggcgccctc gccggtccca ccatcgcatc cggtcgggca gttcccgccc
    15481 ggtaccggcg cggctcgctc ggcgcgcctc gcccgaagga gacagggtac cgtgggccac
    15541 gtcatcggga tcgacctggg gtcgcagagc ctcaagggac tcctgctcga tcccgcgggg
    15601 cggctgatcg gggaggcgtc ccgatcctac gacctcgcct acccgttccc ggcctgggcc
    15661 cagcaggacc cggccgactg gtggacggcg atccgcgccg tcgtcggcga gctcgtggcc
    15721 gcggcggccc tcgcccccgg cgatgtgtcg gcgctggcgt tcgcaagcca ggtcgacggg
    15781 gtcgtcgccg tcgacgtcga aggcgaagac ctccacccgg cgatcatctg gctggaccgc
    15841 cgggccgagg ccgaggccgc cgctctcggt cggcggatct cgccggaagc gctcttcgag
    15901 cggtcagggc tgaatctcga ttcgagccac gtcgccccca agatcctctg gcttcgggcc
    15961 cacgtgcccg atgcgtatgc ccgcgccgcg acgctccacc tcccgggaag ctgggcggtc
    16021 gcccgcctca ccggcgtatc cgtcgtcgat cattcgaacg cctcctcgac gcttctctac
    16081 gacgtccgga cccgtgactg gtcggacgag ctgcttgccg ccgccgacgt cgagcggtcg
    16141 cgtctccccg tcgtggccgc atcgacggcc gtggccggga ccctgaccgc gcacgccgcc
    16201 gccgagctcg ggctcacgac ggcctgtctc gtggccgtcg ggtcgggcga cgagcacgcc
    16261 gcctgcctgg gggcgggcgt cctgcgagcc gggccggtct gcgacatcgc cggcacggcc
    16321 gagccggttg ccgtcgcatc cgacctcccg gtgttcgacc gaacggggct cgtcgagacc
    16381 cacgcccacg ccgatccgcg gagctggctg atcgagaacc cggggttcgt ctcgggcggc
    16441 agcgtccgct ggttcaacga cacgatcggt cgcatccttc gcgatgtcgg tgatcgtgcc
    16501 ggacccgacg ctgacgacgc agccggcgcc ctcgatcgcg gaatcggcct cggccagggc
//
LOCUS       DATAIW010000449         4996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_195475, whole genome shotgun sequence.
ACCESSION   DATAIW010000449 DATAIW010000000
VERSION     DATAIW010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195475"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VHM48_13470"
     CDS             complement(<1..351)
                     /locus_tag="VHM48_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2756472.1"
                     /translation="MAVTITAPNQTSTATPTIRAIGLCKAFGPIVAVDDVSFELAAGR
                     IYGLLGPNGSGKTTLIRLLTGLGRADAGTAEVLGARMPSRVTLGQIGYMTQSDGVYPA
                     LTVAENVRFFGTVYG"
     gene            438..1169
                     /gene="cmk"
                     /locus_tag="VHM48_13475"
     CDS             438..1169
                     /gene="cmk"
                     /locus_tag="VHM48_13475"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2756473.1"
                     /translation="MTTNPPGLVVALDGPGSSGKSSVGAAAALALGYRFCDTGLLYRA
                     ATWLALSRRVRAEGAGADPAALVALVPEIALIADANGRLAHVSVNGVDVTADVHTPRV
                     DDAVSAVSRVPELRAALLERQRALAAEPGGIIMAGRDIGTVVLPHADLKIFLDASVEE
                     RARRRTESREIATDSPEARWILAQLRRRDNLDSTRAVAPLRPADDAVHIVTDGNRLED
                     TVRAVVGEIRRAEATRSVGAGEPAR"
     gene            1166..1915
                     /locus_tag="VHM48_13480"
     CDS             1166..1915
                     /locus_tag="VHM48_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2756474.1"
                     /translation="MSARSTEPASHRPSAKPVLTEDELLRSDITALIRSIAFGTRVIC
                     RCYTRVRIEGDSDAIPRDGPVILAANHISNADAVLIGAFLTPRLGRRIHWLGKKEMFD
                     WPLVGFIARNGGVVPVDRGAADVEAFRVARRILDAGNVLMVFPEGTRSPTGELQPPKD
                     GLAMLALRTDATIVPIGISNTDRIWPRGRPIPFPGKHATMRIGAPFKVGKLLPPELDR
                     KAAKGLATTLIMRRIAALLDLRHRGTYGEDG"
     gene            1935..2321
                     /locus_tag="VHM48_13485"
     CDS             1935..2321
                     /locus_tag="VHM48_13485"
                     /inference="COORDINATES: protein motif:HMM:NF039679.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HEX2756475.1"
                     /translation="MRKDPYELPLAGRIVSAMAFSRAGYPQRLEFWADGNDRPQHLLV
                     IEGDVQLRAGTTSWKGHPNEPEMLALLSQRVQSASANATGDLIISFESGDVLEVDAGA
                     YESWQFEDGAGGSAVSVAGGGLAVWD"
     gene            2575..3561
                     /gene="ispH"
                     /locus_tag="VHM48_13490"
     CDS             2575..3561
                     /gene="ispH"
                     /locus_tag="VHM48_13490"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2756476.1"
                     /translation="MGSVREIRIARRTGFCYGVREAIDKAKEASAAGKSTHTLGQVVH
                     NEGVIADLERLGIETVETLDDVDHGAAVVIRAHGVRPDVMERANERGLEVIDGTCTWV
                     IQEHRELVKLVDEGYTIVLLGTPNHAEVVGMLGFAPDAIVVDEEDEWEQKVPRKKRLA
                     LISQSTQPPWKFEKLAAFMVGRSHELKIVNTVCPVTIRRQEDTVEAAQAVDLMVVVGG
                     RSSANTKELTRLVGIAGKPAIQIEGASDLTDPSVFDGRQVIGITGGTSTPIEDLRDVA
                     QRVFEIAGTPDTAAHAPELAEEALADAATPAGRTTSLPTFVHNEDAAASGAA"
     gene            3567..>4996
                     /gene="der"
                     /locus_tag="VHM48_13495"
     CDS             3567..>4996
                     /gene="der"
                     /locus_tag="VHM48_13495"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX2756477.1"
                     /translation="MPSRRLAALPVLAVVGRPNVGKSTIFNRFVGKRQAIVEDRARTT
                     RDRMYGDMEWNGRRFVIIDTGGLEVDTDDPIEIRVQEQARLAIREADVILFVVDAISG
                     LTPADLEAAELLRTARAPIIVAVNKVDNTQREADAAEFWSMGWEETYSISASHGRGTG
                     DLLDAIVWALPPETEAELARKKREDEADEWAREVDAGRLEPYVVGDAGNAADPDEDEE
                     AEQAELDRFEGGQAAGGEIEARWDAAIEAESDDVQAAIAFVGRPNVGKSSLLNSLLGE
                     DRAIVSEVPGTTRDAVDTTLEWGRSEIVLIDTAGIRRRGKVANGPAAEKYSTLRALKA
                     IARSDVAVLVIDAVEGLTSQDAHVAGYVVDEGKGLVIAVNKWDLVEDKTDKTFTQYTE
                     WIRSEVPFLEFAPIVSISAKTGQRVGKVLELAIDIWGERRKRISTGELNRIIRAAVER
                     QSPPVVKGRRPKVFYGTQAAVAPPTFV"
BASE COUNT          798 a         1810 c         1687 g          701 t
ORIGIN      
        1 gccatagacc gtgccgaaga agcggacgtt ctcggcgacc gtcagcgccg ggtagacgcc
       61 gtcgctctgg gtcatgtagc cgatctgacc gagcgtcacc cgtgacggca tccgcgcgcc
      121 gagcacctcg gccgtgcccg cgtccgccct cccgagcccc gtcaggagcc ggatcagggt
      181 cgtcttgccc gagccgttcg ggccgagcag gccgtagatc cgaccggccg ccagctcgaa
      241 gctgacgtcg tcgaccgcga cgatcgggcc gaacgccttg cacaggccga tcgcccggat
      301 cgtcggcgtg gcggtcgatg tctggttcgg cgcggtgatg gtgacggcca tctggaccct
      361 gccctccgat gcgatcgtaa ccccgccgtg aaccacccga ccggtccgaa cggcccgtgg
      421 ccgctatgct cgacgtcgtg accaccaacc cgcccggtct cgtcgtcgcg ctcgacggtc
      481 ccggctcgtc cggcaagtcg agcgtcggtg ccgcggcggc cctcgcgctc ggctatcgct
      541 tctgcgacac gggcctgctg taccgcgcgg cgacctggct cgccctctcc cgccgtgtcc
      601 gggcggaggg cgccggcgcg gacccggcgg cgctcgtggc gctcgttccg gagatcgccc
      661 tcatcgccga cgccaacggg cgtcttgccc acgtcagcgt caacggcgtc gacgtgaccg
      721 cggacgtcca cacgccgcgg gtcgatgacg ccgtctcggc cgtcagccgc gtgccggagc
      781 tgcgggcggc cctcctggag cggcagcgcg ccctcgccgc ggagcccggc gggatcatca
      841 tggccgggcg cgatatcggg actgtcgtcc tgccccacgc cgacctgaag atcttcctcg
      901 acgcctcggt cgaggagcgc gcccggcgac ggacggaatc ccgcgagatc gccaccgaca
      961 gccccgaggc ccgctggatc ctggcccagc tccgtcgccg cgacaacctc gacagcaccc
     1021 gcgccgtcgc gccgctccga cccgcggacg acgcggttca tatcgtgacc gacgggaatc
     1081 gcctcgagga cacggtccgg gcggtcgtcg gcgagatccg gcgcgccgag gctacgcgat
     1141 cggtcggggc aggggagccc gcccgatgag cgctcgatcg accgagcccg ccagccatcg
     1201 cccgtccgcg aaacccgtcc tgaccgagga tgagctcctc cgcagcgaca tcacggcgtt
     1261 gatccggtcg atcgcgttcg gcacgcgcgt catctgccgc tgctacacgc gggtccggat
     1321 cgagggcgat tccgacgcga tcccgcgcga cggcccggtc atcctcgccg ccaaccacat
     1381 ctccaacgcc gacgcagtgc tgatcggggc attcctcacg ccgcgtctcg gccggcggat
     1441 ccactggctc ggcaagaagg agatgttcga ctggccgctc gtcggcttca tcgcccgcaa
     1501 cggcggcgtc gtgccggtcg accgcggggc agccgatgtc gaggcgttcc gggtcgcccg
     1561 ccggatcctc gacgcgggca acgtcctgat ggtcttcccc gagggcaccc gcagcccgac
     1621 cggcgagctc cagccgccca aggacggcct cgcgatgctg gcgctccgga ccgacgcgac
     1681 gatcgtcccg atcggcatct cgaacaccga ccggatctgg cccaggggcc gcccgatccc
     1741 attcccgggc aagcacgcca cgatgcggat cggcgcgcca ttcaaggtcg gcaagctgct
     1801 cccgccggag ctcgaccgga aggccgccaa gggcctcgcc acgaccctga tcatgcgccg
     1861 gatcgccgcc ctcctcgacc tgcgccaccg cggcacctac ggcgaggacg ggtaagacat
     1921 tgatccggcc cgacatgcgc aaggacccct acgaactccc actcgcagga cggatcgtca
     1981 gcgcaatggc tttctccagg gccggctatc cgcagcgatt ggaattctgg gccgacggga
     2041 atgatcgacc ccagcatctg ctcgtgatcg aaggagacgt ccagcttcgc gcgggaacga
     2101 cctcctggaa gggtcacccg aacgagcctg aaatgcttgc gctgctgagc cagcgggttc
     2161 agtcggcgtc ggccaacgct acgggtgatc tgatcatcag cttcgaaagc ggcgatgtcc
     2221 tcgaagttga tgccggcgca tatgagagct ggcaattcga ggacggagcc gggggctcgg
     2281 cagtgagcgt tgcgggcgga ggcctggctg tttgggactg acgcccgtgt cctcggccca
     2341 attgttggcc agatatgcgc ccggggactc ttgggccaga ttcatcggcc gatttggcgc
     2401 tgacggggcc ccgcgttagc cctcgaaagc taactgcgcc gcattcgcgc cacaggtccc
     2461 ggccgaacag tctcccgatg accatctggc caagtttcgc caggaagagc cgtccgccgc
     2521 gcggcccgga cccctgaccg ggtttcggca cctcgcatgc gataatcggc acgtatggga
     2581 agcgtccggg agatccggat cgcccgacgc accggcttct gctacggcgt gcgcgaggcg
     2641 atcgacaagg ccaaggaagc cagcgccgct ggcaagtcga cccataccct cggccaggtc
     2701 gttcacaacg agggcgtcat cgccgacctg gagcggctcg gaatcgagac cgtcgagacc
     2761 ctcgacgacg tcgaccacgg tgccgcggtc gtgatccgtg cccacggtgt ccggccggac
     2821 gtcatggagc gcgccaacga gcgcggtctc gaggtcatcg acggcacctg cacctgggtg
     2881 atccaggagc accgcgagct ggtcaagctc gtcgacgagg gctacacgat cgtcctcctc
     2941 ggcacgccga accacgccga ggtcgtcggg atgctcggct tcgcgcccga cgcgatcgtc
     3001 gttgacgaag aggacgagtg ggagcagaag gtgccccgca agaagcgcct cgccctcatc
     3061 tcccagtcga cccagccgcc gtggaagttc gagaagctcg ccgcgttcat ggtcggccgg
     3121 agccacgagc tgaagatcgt caacaccgtc tgcccggtca cgatccgccg ccaggaggac
     3181 acggtcgagg cggcccaggc cgtcgacctg atggtcgtcg tcggtggccg ctcgagcgcg
     3241 aacacgaagg agctgacccg cctggtcggc atcgccggca agcctgcgat ccagatcgag
     3301 ggcgcgtcgg acctgaccga cccgagcgtc ttcgacggcc gccaggtcat cggcatcacc
     3361 ggcggcacct cgaccccgat cgaggacctc cgcgacgtcg cccagcgcgt gttcgagatc
     3421 gccggcacac ccgacaccgc cgcccacgcg ccggagctgg ccgaggaggc gctggccgac
     3481 gccgcgaccc cggccggtcg gacgacctcg ctgccgacct tcgtccacaa cgaagacgcg
     3541 gccgcgtccg gcgcggcctg accgccatgc cttctcgccg gctcgcggcc ctgccggtcc
     3601 ttgccgtggt cggccgtccc aatgtgggca agagcacgat cttcaaccgc ttcgtcggca
     3661 agcgccaggc gatcgtcgag gatcgcgccc ggacgacccg cgaccggatg tacggcgaca
     3721 tggagtggaa cggccggcgg ttcgtcatca tcgataccgg cgggctcgag gtcgacacgg
     3781 acgatccgat cgagatccgg gtccaggagc aggcccgtct cgcgatccgc gaggctgatg
     3841 tcatcctctt cgtcgtcgac gcgatcagcg gcctgacccc ggccgacctc gaagccgccg
     3901 agctccttcg gaccgccagg gcgccgatca tcgtcgcggt caacaaggtc gacaacaccc
     3961 aacgcgaggc cgacgcggcc gagttctggt cgatgggctg ggaggagacg tactcgatct
     4021 ccgcttccca tgggcgcggc accggtgacc tcctcgacgc gatcgtctgg gcccttccgc
     4081 ccgagaccga ggccgagctc gcccgcaaga agcgcgagga cgaggccgac gagtgggccc
     4141 gcgaggtcga cgccggccgg ctcgagccgt acgtcgtcgg cgatgccggg aatgcggccg
     4201 atcccgacga agacgaggaa gccgagcagg ccgagctcga ccggttcgag ggtggccagg
     4261 ccgccggcgg cgagatcgag gcccgctggg acgccgccat cgaggccgag tccgacgacg
     4321 tccaggcggc gatcgcgttc gtcggccggc cgaacgtcgg caagtcgtcg ctcctcaaca
     4381 gcctcctagg cgaggaccgg gcgatcgtgt cggaggtccc ggggacaacc cgcgacgcgg
     4441 tggacacgac cctcgaatgg ggccgcagcg agatcgtcct catcgacacc gccgggatcc
     4501 ggcgccgcgg caaggtcgcc aacggcccgg ccgccgagaa gtactcgacc ctccgcgcgc
     4561 tcaaggcgat cgcccgttcc gacgtcgcgg tcctcgtcat cgatgcggtc gaggggctga
     4621 cgtcgcagga cgcccacgtt gcgggctatg tcgtcgacga gggcaagggc ctcgtcatcg
     4681 cggtcaacaa gtgggacctc gtcgaggaca agaccgacaa gacgttcacc cagtacaccg
     4741 agtggattcg gagcgaggtg ccgttcctcg agttcgcccc gatcgtgtcg atcagcgcca
     4801 agaccggcca gcgggtcggc aaggtgctcg aactcgcgat cgacatctgg ggcgaacgtc
     4861 gcaagcggat ctcgaccggc gagctgaacc gaatcatccg ggccgcggtc gagcgccagt
     4921 cgccgccggt cgtcaagggc cgccgcccga aggtcttcta cgggacgcag gccgcggtcg
     4981 ccccgccgac gttcgt
//
LOCUS       DATAIW010000450         5120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_225215, whole genome shotgun sequence.
ACCESSION   DATAIW010000450 DATAIW010000000
VERSION     DATAIW010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225215"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..924)
                     /locus_tag="VHM48_13500"
     CDS             complement(154..924)
                     /locus_tag="VHM48_13500"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX2756478.1"
                     /translation="MHDPFDRSFRDRTLAGETLFGLFLDLASPASAELCAAAGYDWLL
                     VDLEHGASTEADLLGLLHAVEVGGSTPLVRPQSGERIRIGRALDMGARGIMVPRLESA
                     AQADEAVTFLRYPPDGIRGVATRVRGAGLGVVPHADVKRLNERVVGIIQIESVGGLHD
                     ADAIAAIDGVDVLFVGPADLSHSLGIPGQFQHADYSGALERVVAACRAHGKAAGILVY
                     DTAVVPRHLELGFTFVGIGADGAFVADTAKRFLATVRG"
     gene            complement(1261..2484)
                     /locus_tag="VHM48_13505"
     CDS             complement(1261..2484)
                     /locus_tag="VHM48_13505"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX2756479.1"
                     /translation="MYYVAQGSIPRQRHTQHRAPDGSLYAEELFGVEGFTGRSSLLYH
                     LTPPTRTHKIEAVRRIELEAADDGLHRHRLTKTGAVEPKGDIVTGRIPLYFNHDVVFG
                     IVRPAEPMPAGRFYRNGIADEMLFIHEGTGVCDTIFGPLRYGPGDYLVLPIGTTWRLD
                     PDPGSEQRILFLEAPSEIEPPKRYRNDYGQLLEHSPYNQRDIHAPALRPASHEEGEFV
                     IDVRSTDRITAYHYRHHPFDVVGWDGYLWPFRFNIADFQPITGRVHQPPPVHQTFQAR
                     NFVVCSFVPRKFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVDIASFTLHPAG
                     IPHGPHPGTVEASIGKDATEELAVMVDTFHPLHVTKAAMDLDDDRYPYSWLPPEEGSD
                     EAAHLLERGPEAFPD"
     gene            complement(2542..3891)
                     /locus_tag="VHM48_13510"
     CDS             complement(2542..3891)
                     /locus_tag="VHM48_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756480.1"
                     /translation="MTDDRSLERAARSFIEAGPTRAPEAAVEGALLLIDTTPQERDLR
                     IPLRFSTMTTPARVAAAAVIGVLAIGGASFLIGRSGQAPNVGGPSPSAPQPSPSTTQS
                     ATPLASSSPSSAAQVDYSTIKGTVLMEHLGNAPDLSEMPTTDYHAERRRLYFMDPATM
                     TGPTAREFLPNQPPTGKLNADISSDGKKVVFMDTADPAAIWLANVDGTGLRKLSTACS
                     CSELDPAFDPTGTKIAFVHLEGAWRWSRNGAQLRLESRAGPSTSWLGVRDLTTGKVTK
                     LDQTVGPSNDAVPEQPSWSPDGKKIVFNRTTWGSTDFPEHGVLQIVDVTSGKVTTLTA
                     QMTLPGDADWSPDGSRILYTNYPWSSMGSIGGLPNSQLVSILPDGTGLLGLPAGVGGS
                     WMPDGRILFQAQRAAGGGYFWIINADGSNSRPVNLSGDSLSDLPQGFSYIPHWIAAP"
     gene            complement(3888..4439)
                     /locus_tag="VHM48_13515"
     CDS             complement(3888..4439)
                     /locus_tag="VHM48_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2756481.1"
                     /translation="MDRDLVEQARSGDREAFAVLVHQVSDSLYAVAHRILRDSGLAED
                     ALQNALVLAWRRLPHLRDADRFEAWIHRILVHACYDESQRSRSWTANVRVLPIEGPSA
                     PDGSDAVADRDELERAYRLLSIEQRAVFVLHHYLGLPLVEVADLLGIPAGTARSRLHY
                     AIAGLRDALTAEPEPIVREGHLA"
     gene            complement(4727..>5120)
                     /locus_tag="VHM48_13520"
     CDS             complement(4727..>5120)
                     /locus_tag="VHM48_13520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756482.1"
                     /translation="PDWAHWHTSHRRDCRFVARLDGRVVGWTALGRYSTRAVYEGVAW
                     ESVYVDAAVRGWGVGSVLLAALIPASESAGVWTLLAGVQANNPASLALHERAGFRRIG
                     VQERIGRDATEIWRDVVLLERRSNVVGS"
BASE COUNT          766 a         1702 c         1882 g          770 t
ORIGIN      
        1 ggtgcgtgcc ggcgattcgg gccttcgacc gccgacgacg ggccacgtcc ggccgcgcac
       61 gtgacgtcga ccacgggcga tggtgcatga cgagaaaccg gggccgaacc ggcgcgatcc
      121 ggcgcgatcc ggcgcgcacc ccgcggccca gcctcagccg cggaccgtcg ccaggaaccg
      181 cttcgcggtg tcggcgacga acgcgccgtc cgcgccgatc ccgacgaacg tgaacccgag
      241 ctcgaggtgg cgagggacga cggcggtgtc gtagacgagg atgccggccg ccttgccgtg
      301 agcccggcac gcggcaacca cccgctcgag ggcgccgctg tagtcggcat gctggaactg
      361 gcccggaatc ccgagcgagt gcgacaggtc ggccgggccg acgaagagca cgtcaacccc
      421 gtcgatcgcg gcgatcgcgt cggcgtcgtg caggccgccg acggattcga tctggatgat
      481 cccgacgacc cgctcgttca ggcgcttgac gtcggcgtgc gggaccacgc cgagaccggc
      541 gccgcggacc cgggtcgcga cgccgcggat gccgtccggc gggtagcgca ggaacgtgac
      601 ggcctcgtcg gcctgtgcgg cggactcgag gcgcggaacc atgatcccgc gggcgcccat
      661 gtcgagggcc cgcccgatcc ggattcgctc gccggactgg ggccggacga gcggcgtcga
      721 accgccgacc tccaccgcgt ggagcaggcc gagcaggtcg gcctcggtcg acgcaccgtg
      781 ctcgaggtcg acgagcagcc agtcgtagcc ggccgcggcg cacagctcgg ccgaggccgg
      841 cgaggcgagg tcgaggaaca ggccgaacag cgtctcgccg gcaagcgtgc ggtcgcggaa
      901 ggaccgatcg aaggggtcgt gcatcctcgt atcatgcccc gccgccgccc cggtcgatcg
      961 atcgggccgc aactggggaa cgaaggttga accgcgcgcc cgacgggacc gcccggcgaa
     1021 ccctcagtcg gccccagtgg agacccgagc ggagagtgca cgctcacgat cccgaccgtt
     1081 cggctgatta ccgtcgatgg tcgtcaggga cggatggtga gcaacgactg ccccccccga
     1141 atcacaggcg ttcgtcttca tcggacagcg cgtgtacgtc ttcgcgatcc tcgatgtcaa
     1201 cctgcgcgga ctcctccccg acattcctgt cgactgtgaa gctccagccg taagccgacc
     1261 ttagtcgggg aaggcctcgg gcccgcgctc gagcaggtgg gcggcctcgt ctgagccctc
     1321 ctcgggcggc agccagctgt acgggtagcg atcgtcgtcg aggtccatcg ccgccttcgt
     1381 gacgtggagc gggtggaagg tgtcgaccat gaccgccagc tcctccgtcg catccttgcc
     1441 gatcgatgcc tcgaccgtgc ccgggtgggg accgtgcggg atccccgccg gatggagggt
     1501 gaacgaggcg atgtccacgc cgcgccggct catgaagttg cccgcgacgt agtagatgac
     1561 ctcgtcgctg ttgatgttcg agtggttgta tggcgccggg atcgcgagcg ggtggtagtc
     1621 gaacttgcgc ggcacgaacg agcacacgac gaagttcctc gcctggaatg tctggtggac
     1681 cggcggaggc tggtgaacgc gccccgtgat cggctggaag tcggcgatgt tgaagcggaa
     1741 gggccagaga tagccgtccc agccgaccac gtcgaacggg tgatggcggt agtggtaggc
     1801 agtgatccga tcggtcgagc ggacgtcgat gacgaactcg ccctcctcgt ggctggccgg
     1861 cctgagcgcc ggggcgtgga tgtcgcgctg gttgtacggg ctgtgctcga gcagctggcc
     1921 gtagtcgttg cggtagcgct tcggcggctc gatctcggac ggcgcctcga ggaacaggat
     1981 ccgctgctcg ctcccgggat cggggtccag ccgccacgtc gtcccgatcg gcaggacgag
     2041 atagtcgccc gggccatagc gcaacggccc gaagatcgtg tcgcagacgc ccgtcccttc
     2101 atggatgaac agcatctcgt cggcgatccc gttgcggtag aaccggcccg ccggcatcgg
     2161 ctcggccggc cggacgatcc cgaacacgac gtcgtggttg aagtagagcg ggatgcgccc
     2221 ggtgacgatg tcacccttcg gctcgacggc gcccgtcttc gtcaggcgat ggcggtggag
     2281 accgtcatcg gcggcttcga gctcgatccg gcggaccgcc tcgatcttgt gcgtccgcgt
     2341 cggcggcgtg aggtggtaca ggagcgagct ccggccggtg aagccctcga ccccgaacag
     2401 ctcctcggcg tagaggctgc cgtccggcgc gcggtgctgg gtgtggcgct gccgggggat
     2461 gctgccctgg gcgacgtagt acatgaagcg actggcctcc tcgagtgaga aggccagtct
     2521 agaaggggtg gcgccggccg gtcagggcgc ggcgatccag tgggggatgt atgagaagcc
     2581 ctgcgggagg tcggacaggc tgtcgccgct gaggttgacc ggccgcgagt tcgagccgtc
     2641 ggcgttgatg atccagaagt agccgccgcc tgccgcacgc tgtgcctgga acaggatccg
     2701 gccgtccggc atccaggacc cgccgacgcc agcggggagc cccaggaggc cggtgccgtc
     2761 gggcaggatc gagacgagtt gcgagttcgg aagcccgccg atgctgccca tcgatgacca
     2821 cgggtagttc gtatacagga tcctggagcc gtccggcgac caatcggcgt caccgggcaa
     2881 cgtcatctgg gcggtcaggg tcgtcacctt tccggacgtc acgtcgacga tctggaggac
     2941 gccatgctcg gggaagtcgg tactgcccca tgtcgtccgg ttgaacacga tcttcttgcc
     3001 gtcgggcgac cacgagggtt gctcgggcac cgcgtcgttc gacgggccga ccgtctggtc
     3061 gagcttcgtg accttcccgg tggtcaggtc ccggacgccg agccacgacg tcgacgggcc
     3121 cgcgcggctc tcgagacgga gctgggcccc gttcctcgac caacgccacg ccccctcgag
     3181 gtggacgaag gcgatcttcg tccccgtggg atcgaaggcc ggatcgagct cgctgcagct
     3241 gcaggcggtc gacagcttcc gcaggcccgt gccgtcgaca ttcgccagcc agatcgcggc
     3301 gggatccgcc gtgtccatga acacgacctt cttgccgtcg gacgagatgt cggcgttgag
     3361 cttgccggtc ggcggctggt tgggcaggaa ctcgcgggcg gtcggtccgg tcatcgtcgc
     3421 cgggtccatg aaatagagcc gccgccgctc ggcgtggtag tcggtcgtcg gcatctccga
     3481 caggtccggg gcattgccga gatgctccat caggaccgtg cccttgatcg tcgagtagtc
     3541 gacctgggcc gcgctcgatg gcgacgacga ggccagcggg gtcgcggatt gggtggttga
     3601 cgggctcggc tgcggcgccg acgggctcgg ccctccgaca ttcggcgcct ggccgctcct
     3661 cccgatgagg aatgaggcgc cgccgatcgc tagcacgccg atcacggcgg ccgcggcgac
     3721 gcgggctggg gtggtcatgg tcgagaacct cagcgggatc cggagatccc gctcttgtgg
     3781 ggtggtgtcg atcagcagga gggcgccctc gacggcggcc tccggcgccc gggtcggacc
     3841 tgcctcgatg aacgatcggg cggcgcgttc gagcgagcga tcgtcggtca tgcgaggtgt
     3901 ccttcccgga cgatgggctc cggctcggcc gtcaaggcgt cgcggagccc ggcgatggcg
     3961 taatggaggc gcgatcgggc ggtcccggcc gggatgccga gcaggtcggc gacctcgacc
     4021 aagggcaacc cgaggtagtg gtgcaggacg aagaccgccc gctgctcgat cgagagcagg
     4081 cgatacgccc gctccagctc gtcgcggtcg gcgacggcgt cgctcccgtc cggggccgac
     4141 ggcccctcga tgggcaggac ccggacgttt gcggtccacg aacgggaccg ctgcgactcg
     4201 tcgtagcaag catggacgag gatccgatgg atccacgcct cgaagcggtc cgcatcgcgg
     4261 agatgcggga gccgccgcca ggcgaggacg agggcgttct gcagcgcgtc ctcggccaga
     4321 ccgctgtcgc gcaggatccg atgggcgacc gcgtagagcg agtcgctgac ctggtgcacc
     4381 aggacggcaa acgcctcgcg atcacccgag cgggcctgct cgacgaggtc tcgatccacc
     4441 tcgacctccg ggtcgccgcg gcctcgatgg ccgcggacta cccactatga cgggaggtgt
     4501 cgggcggaac gttcagtcgg aggcccgatc gggtcaatcc ggcccatggt tcgcggatat
     4561 ggcgcgtttc gggtccgtcg acgttccgtg gcggtgcctg cgtcgaccgt cgggacgtcg
     4621 gcggcctcgt ttgcgtctct gtcatgacca ggagatgccg ctcgcggccc tcgcgatccg
     4681 ttcggccctg gcgttcctcg gtctgcgccg gtcaggagcc gacgggtcaa ctgccgacga
     4741 cgttgctccg gcgctcgagc aacacgacgt cgcgccagat ctcggtcgcg tcgcggccga
     4801 tccgctcctg gacgccgatc cggcggaacc cggcccgctc gtggagggcg agactggccg
     4861 ggttgttggc ctggacgccg gcgaggaggg tccacacgcc ggccgattcg gacgccggga
     4921 tcaacgcggc cagcagcacc gagccgacgc cccagccgcg caccgcggcg tcgacgtaga
     4981 cgctttccca ggcgacgcct tcgtagaccg cacgggtcga atagcgcccc agcgccgtcc
     5041 agccgacgac gcggccgtcg agccgggcga cgaaccggca atcgcggcga tgcgaggtgt
     5101 gccagtgggc ccagtcgggc
//
LOCUS       DATAIW010000451         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_195818, whole genome shotgun sequence.
ACCESSION   DATAIW010000451 DATAIW010000000
VERSION     DATAIW010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195818"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VHM48_13525"
     CDS             complement(<1..596)
                     /locus_tag="VHM48_13525"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX2756483.1"
                     /translation="MTEFATRVDAFFDEYFAREPTDATGAGNHAYDGLWPDLAAAARP
                     DRLAFIDRWRATFETFADDALDVDERVDRDLLLGVLASYRFNEVELREDAWSPMAWVY
                     LVGQGFFSLIAREFAPLADRLGSIASRAERLPPLLDAASEALVGHAGRPVDRFHTETA
                     LDQWPGLIGVVDEAMVAGETAAADGDEAVSPATIASSTT"
     gene            693..1289
                     /locus_tag="VHM48_13530"
     CDS             693..1289
                     /locus_tag="VHM48_13530"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2756484.1"
                     /translation="MDASRRVEPIILEGRRVRLEPLALEHVAGLAEVALDPALWQWTL
                     ARPTDEAGVRAWAVAALAGRDAGTEFPFVTLDAGTGRPIGSSRFMNIVLEHRRLEIGW
                     TWVAPAWQRTGANREAKLLMLGHAFDTLGCRRVEFKTDSLNEPSRTALLGIGAQFEGI
                     FRNHMVMPDGRMRHSAYYSVIDEEWPDVRAGLKRSLAR"
     gene            1353..1760
                     /locus_tag="VHM48_13535"
     CDS             1353..1760
                     /locus_tag="VHM48_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2756485.1"
                     /translation="MVELIVKILINAAALFVAVKLLPSNLLSFNWGNDWWKLLLVALV
                     FALVNSYIKPIVKALSMPIGLLTLGLIGFVINAGMLLLTALASESLKLGFKLGTFPPT
                     INSDAIVGALVAALIISVVSTVATVALTPRKLM"
     gene            1764..>2113
                     /locus_tag="VHM48_13540"
     CDS             1764..>2113
                     /locus_tag="VHM48_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756486.1"
                     /translation="MPRSGIAGSVGGPVEPIDRLPAIAGALREAAARYGTPLYLTDAA
                     TVTTAAAELHDAFPDPWIRQYSVKANDVAGVIALAAGGAPGLGANVVSRGEWAAARRA
                     GVPNARISLEGIGKT"
BASE COUNT          303 a          753 c          749 g          308 t
ORIGIN      
        1 gtggtcgacg aggcgatggt ggccggcgag acggcctcgt cgccgtccgc cgcggccgtc
       61 tcgccggcca ccatcgcctc gtcgaccacc ccgatcagac ccggccactg gtcgagggcg
      121 gtctcggtgt ggaagcgatc gaccgggcga ccggcatgtc cgacgagcgc ttcggacgcg
      181 gcatcgagga ggggcggaag tcgctcggcc cgcgacgcga tcgatccgag ccggtccgcc
      241 agcggcgcga actcccgggc gatcaggctg aagaacccct ggccgaccag gtagacccag
      301 gccatcgggc tccaggcgtc ctcgcgcagc tcgacttcgt tgaagcgata ggacgcgagc
      361 acgcccagga gcaggtcgcg atccacccgc tcgtcaacgt ccagcgcgtc gtcggcgaac
      421 gtctcgaagg tcgcccgcca gcgatcgatg aacgcgaggc ggtcgggccg ggcggctgcc
      481 gcaaggtccg gccagaggcc gtcgtaggca tggttgccgg cgcccgtggc atcggtcggc
      541 tcgcgggcga agtactcgtc gaagaacgcg tcgacgcgcg tggcgaactc ggtcacgggc
      601 ggcctccggt gtcgtgtggc ggctcggacg ggcgcttcaa ccgacacgag cgggaagggc
      661 gtcgagtcta gcggagcgtc taccatcggg ggatggacgc cagtcggcgg gtcgagccca
      721 tcatcctcga ggggcgccga gtgcgcctcg agccgctggc gctcgagcac gtggcgggcc
      781 tcgcggaggt cgccctcgac ccggcgctct ggcaatggac cctcgcccgc ccgaccgacg
      841 aggccggcgt ccgcgcctgg gcggtcgcgg cgctcgccgg tcgcgacgcc ggcacggagt
      901 tcccgttcgt gacccttgac gcggggaccg gccggccgat cggcagcagc cggttcatga
      961 acatcgtcct cgagcatcgc cggctcgaga tcggctggac ctgggtcgca ccggcctggc
     1021 agcggacggg cgccaaccgc gaggcgaagc tgctgatgct gggccacgcg ttcgacacgc
     1081 tcggctgccg gcgggtcgag ttcaagaccg actcgctcaa cgagccgtcg cggaccgcgc
     1141 tgctcgggat cggggcgcag ttcgagggga tcttccgcaa ccacatggtc atgccggacg
     1201 gccggatgcg gcactccgcg tactacagcg tgatcgacga agagtggccg gacgtccgtg
     1261 ccgggctgaa gcggtcgctc gcgcgctgat tccatctgcg atgatcgacg ggccgcgccc
     1321 ggccggcgcg ccacatccga tcggagggtc ccatggtcga gctcatcgtc aagatcctga
     1381 tcaacgccgc cgccctgttc gtcgccgtca agctcctgcc gtcgaacctg ctgtcgttca
     1441 actggggcaa cgactggtgg aagctgctcc tggtcgccct cgtcttcgcc ctcgtcaaca
     1501 gctacatcaa gccgatcgtg aaggccctgt cgatgccgat cgggctgctg acgctcggcc
     1561 tgatcggctt cgtcatcaac gccgggatgc tgctcctgac cgcgctcgca tccgagagcc
     1621 tgaagctcgg cttcaagctc ggcaccttcc cgccgacgat caactccgac gcgatcgtcg
     1681 gcgccctggt cgcggccctc atcatcagcg tcgtctcgac cgtcgccacc gtcgcgctca
     1741 cgccgcggaa gttgatgtag gcggtgccgc gctccgggat cgccggctcg gtcggcggcc
     1801 ccgtcgaacc gatcgaccgc cttccggcga tcgccggcgc actgcgcgag gccgccgcgc
     1861 gctacggcac gccgctctac ctgaccgacg cggccaccgt cacgacggcc gctgccgagc
     1921 tgcatgacgc cttcccggac ccgtggatcc ggcagtactc cgtcaaggcc aacgacgtcg
     1981 cgggcgtgat cgcgctggca gcgggtgggg cgcccggtct cggagcgaac gtcgtctcgc
     2041 gtggtgagtg ggcggccgct cgcagggccg gggtcccgaa cgcccggatc agcctcgagg
     2101 ggatcggcaa gac
//
LOCUS       DATAIW010000452         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_285506, whole genome shotgun sequence.
ACCESSION   DATAIW010000452 DATAIW010000000
VERSION     DATAIW010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285506"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHM48_13545"
     CDS             <1..465
                     /locus_tag="VHM48_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2756487.1"
                     /translation="EDFQCPACEQWGQNVFPSLVRNELAAGTVKIVYHGFAFIGPESK
                     DAGRAAWAAEQQGHFWDMWATLYANQGLHENGGAFARARLNAMADALGLDAARFAADF
                     DSAAATAFVTDGIADAAKASVNSTPTLIIDGKAFTGSGYADVAAAVAAAAVP"
     gene            462..932
                     /locus_tag="VHM48_13550"
     CDS             462..932
                     /locus_tag="VHM48_13550"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2756488.1"
                     /translation="MTAFATGWRRLRAVHPALILAALDLIGLGIASYLSSVELRGELP
                     NCGPLHGCEQVALSEYSRIGGVPVAVFGVGLSLILFTLAIAWWRTNDGRLLAAHYGLS
                     LLGVVFELYFTYLELFVIGAVCVWCASYGISLVARFLVALWVWIHRERYVIVEP"
     gene            1181..1921
                     /locus_tag="VHM48_13555"
     CDS             1181..1921
                     /locus_tag="VHM48_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756489.1"
                     /translation="MPTQKIFKQRVRARMTKTGESYTAARRQLLRRAGDPGEPEAGEP
                     TIIADPAAAPEPDGLASDVPADAGLSVSDESVRRATGRGHEEWFAILDAWGATERTHT
                     EIAAWLGDVQGVPSWWRQSVTVDYERARGMRGRHEMRDGFSVTATKTIAVDAERALAA
                     FTDVAIRDTWLPDAPLRQRSTRAALTARFDWSDPPSRVVVNVMPKEGGRALVAVAHER
                     VPDAATASRLKGQWRAWLGGLKETIETR"
     gene            complement(2000..>3058)
                     /locus_tag="VHM48_13560"
     CDS             complement(2000..>3058)
                     /locus_tag="VHM48_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756490.1"
                     /translation="FGAHPTDSGPSPAAPVVVYRSADVPPFYLYWTPDGQRVSFLATE
                     ADGISLRVAPADGSAPLDGGPGTVIRQGSPLYFDWVGAERLLLHVGTGPAAFLGEVGL
                     DGSSVAPALDGSGDFRPAVAAASGQYLAFVRGAAPSAQLVVARRDGSTEHATPVFGPA
                     AIVFDPTGDTVATIAADESGQKGLSFPLGPLRLVDAATGAVRTLLDGNVVGFFWSPDG
                     RTVAALRLEGGGGSTVADRQITPAVAISGASEGQPAAALPTTAPTPGSELHLLFVNVA
                     DGAIRSDRIVQLTNQFVRQFLPYFDQYALSHRLWAPDSSSILLPLIGDTGKSQLVAVG
                     ANGDTPRTFDGSSGFWSP"
BASE COUNT          448 a         1072 c         1111 g          427 t
ORIGIN      
        1 gaggacttcc agtgcccggc gtgcgagcaa tggggccaga acgtcttccc gagcctcgtc
       61 cggaacgagc tcgccgcggg aacggtcaag atcgtctatc acgggttcgc gttcatcggc
      121 cccgagtcga aggatgccgg ccgggcggcc tgggcggccg aacagcaggg gcacttctgg
      181 gacatgtggg cgaccctcta cgccaaccag ggcctccatg agaacggcgg cgcattcgcc
      241 cgcgcccgcc tgaacgcgat ggccgatgcc ctgggtctcg acgcggcccg gttcgccgcc
      301 gacttcgact ccgcggcggc caccgccttc gtgaccgatg ggatcgcgga tgccgccaag
      361 gcgagcgtca actcgacccc gaccctgatc atcgacggca aggcgttcac cggctccggc
      421 tatgccgacg tcgcggccgc cgtggctgcc gcagccgtgc cttgaccgcg ttcgccaccg
      481 gctggcggcg ccttcgcgcg gttcacccgg cactcatcct cgccgccctc gacctcatcg
      541 ggctcgggat cgcctcctac ctttcatcgg tcgagttgcg cggcgagctg ccgaactgcg
      601 gcccgctcca cggctgcgag caggtcgcgc tgtccgagta cagccggatc ggcggggtcc
      661 cggtcgccgt gttcggtgtc gggctgtcgc tgatcctgtt caccctcgcg atcgcctggt
      721 ggcgaaccaa cgacggccgg ctgctcgcgg cccactacgg gctgtcgctc ctcggcgtgg
      781 tcttcgagct ctacttcacc tacctcgagc tcttcgtcat cggcgcggtc tgcgtctggt
      841 gcgcgagtta cgggatcagt ctcgtcgccc ggttcctcgt cgccctctgg gtctggatcc
      901 atcgcgagag atacgtcatc gtcgagccgt agcggtcgac cggcggcgtc ctcgccggtt
      961 cggctggatc atcgccgagg cttgtcaacg gccggatcgg ctgcgcatac tgcgaccgac
     1021 ggcgcgtccc gcgccgaacc ccgctccggc gctcacgccg tcggcttcga tggggcaaag
     1081 gggaactcgc ttccccggga ccgctggtcc gcccttctgc ggcgagccgc gccgagcgtg
     1141 agtcccgtgg catcgccgaa cgagaaggat gtcgaggacg atgccgaccc agaagatctt
     1201 caagcagcgt gtccgcgcgc ggatgaccaa gaccggcgag tcgtacacgg ccgcccgccg
     1261 ccagctgctg cgccgcgccg gcgatccggg cgagccagag gctggggagc cgaccatcat
     1321 cgccgacccg gccgctgccc ccgagcccga cggactcgcc tcggacgtgc cggccgacgc
     1381 cgggctctcg gtttccgacg agtccgtccg tcgcgcgacc ggcagaggcc atgaggagtg
     1441 gttcgcgatc ctcgacgcct ggggcgcgac cgagcgaacg cacaccgaga tcgccgcatg
     1501 gctgggcgac gtccagggcg tcccgagctg gtggcggcag agtgtcactg tcgactacga
     1561 gcgggcgcgc ggaatgcgcg gtcgccacga gatgcgcgac gggttcagcg tcactgcgac
     1621 aaagacgatc gcggtcgacg ccgagcgggc gctggcggcc ttcaccgacg tcgcgatccg
     1681 cgacacgtgg ctgccggacg ctccgctccg acagcgttcg acccgggccg cgctgacggc
     1741 ccgcttcgac tggtcggatc cgccgtcgcg cgtcgtcgtg aacgtcatgc ccaaggaagg
     1801 gggcagggcg ctcgtcgcgg tcgcccacga acgggtgccg gacgcagcga ccgccagccg
     1861 cctcaagggc cagtggcgcg cctggctcgg cggactcaag gagacgatcg agacccgctg
     1921 agaggcgacc cgtcggccgc tggcccgggg cgcgctcgcg ccgaccggga tggtcggcgg
     1981 cggcccgtca ccgcccggct cagggactcc agaacccgct cgagccgtcg aaggtccgtg
     2041 gcgtgtcccc gttggcgccg acggcaacca gctgactctt gccggtgtcg ccgatcaggg
     2101 gcaggagaat cgaactgctg tcgggtgccc agaggcggtg gctgagcgcg tactgatcga
     2161 agtagggtaa gaactggcga acgaactggt tcgtgagctg gacgatccga tcggaccgga
     2221 tcgcgccgtc ggcgacgttg acgaagagaa gatggagctc gctgcccgga gtgggcgccg
     2281 tagtcggtag cgccgcggct ggctggccct cgctcgcgcc ggagatggcc acggcagggg
     2341 tgatctggcg gtccgcgacc gtcgagccgc cgcccccctc gaggcgaagg gccgcgacgg
     2401 tccgtccgtc gggcgaccag aagaacccga ccacgttccc atcgagaaga gttcgaacgg
     2461 cccccgtggc ggcatcgacg agacggagcg gaccgagcgg aaaggacagg cccttctggc
     2521 cggactcgtc ggccgcgatc gtggcgaccg tgtcaccggt cgggtcgaag acgatcgcgg
     2581 ccgggccgaa caccggtgtc gcgtgctcgg tcgacccatc gcgacgagcg acgaccagtt
     2641 gggccgaagg cgccgcgccg cggacgaagg ccaggtattg cccgctggcg gccgcgaccg
     2701 ccgggcggaa gtcgccggag ccgtcgagcg cgggcgcgac cgagctgccg tccaggccga
     2761 cttcgccgag aaaggccgcg ggccccgttc cgacatgcag gaggagccgc tccgcgccga
     2821 cccaatcgaa gtagagcggc gagccctgcc ggatgacggt accgggaccg ccgtcgagcg
     2881 gcgcgctgcc gtccgcgggg gcaacgcgga gggagatccc gtcggcctcg gtcgcgagga
     2941 aggagactcg ctgtccgtcg ggcgtccagt agagatagaa gggcggaacg tcggcgctcc
     3001 ggtagacgac gacgggggcc gccgggctcg ggccggagtc ggtcgggtgg gccccgaa
//
LOCUS       DATAIW010000453         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_285559, whole genome shotgun sequence.
ACCESSION   DATAIW010000453 DATAIW010000000
VERSION     DATAIW010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285559"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..803)
                     /locus_tag="VHM48_13565"
     CDS             complement(21..803)
                     /locus_tag="VHM48_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2756491.1"
                     /translation="MILIRAVRRFGDPRPVDAITAAELRAWLLELRSSLAPESIAGYV
                     RGLKAFGNWCVAEELATAPGFRALRRPHVPRRLIAPFSDAELRSLLALADERERALVL
                     LLFDTGLRLSELVSLRVGDVRPDGTLHVLGKGAKERIVPRGTTARRALVRYLGTRARA
                     AVADPLFTGRQGPLSRRGTQQAIARLGRRAGVGTRCSPHTFRHTFARGYLVNGGDVFS
                     LQQILGHATLDMVRRYVTLSEADLVARHRSASPADRLAGARG"
     gene            complement(1068..1142)
                     /locus_tag="VHM48_13570"
     tRNA            complement(1068..1142)
                     /locus_tag="VHM48_13570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1108..1110),aa:Gln,seq:ctg)
     gene            complement(1231..2106)
                     /locus_tag="VHM48_13575"
     CDS             complement(1231..2106)
                     /locus_tag="VHM48_13575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2756492.1"
                     /translation="MAESASLTGRTVLITGGTSGIGRAAAISLAAMGARVGITGRDRV
                     RAEEAAAEIARASGNPAVDVFVADLSSQAEVRRLAGDVLSAYPRLDVLLNNVGGFWAH
                     RHVTADGLEHTFALNHLAPFLLTSLLLERLTASAPARVVTVSSGAQSMGRIDFDDLMG
                     ERKYSGQRAYNQSKLANVMFTYELARRLEGSGVTATVLHPGVTSTAFSAEDPARAFAP
                     LVAVLRPFMRSPERGADTAVHLASSPEVEGVSGRYFADRKARTSHRSSYDTATTARLW
                     RVSADLVGLPVDAVR"
     gene            complement(2106..2849)
                     /locus_tag="VHM48_13580"
     CDS             complement(2106..2849)
                     /locus_tag="VHM48_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756493.1"
                     /translation="MSLLEATNLRKTYRLSRRTGIDALRGVDVSIAAGEMVAIMGPSG
                     SGKSTLMHILGLLHAPDRNDGPAPQLLIDGTDATRLPDRERTRIRAHSMGFIFQSFNL
                     VPTLTAAENVALAAEYAGTSRGRARVAARVALASVGLADRAGHRPMELSGGEQQRVAI
                     ARALVNEPMLLLGDEPTGNLDSARAGEVLGLLRRFNRERGQTVVLVTHDAEVGATCDR
                     IIRMRDGRIVSDERVVADEGGAAVGAAAA"
     gene            complement(2864..>3891)
                     /locus_tag="VHM48_13585"
     CDS             complement(2864..>3891)
                     /locus_tag="VHM48_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756494.1"
                     /translation="VGKIIVNDASSGGIGIGVVPIDLAVADQIRGIAGVAAVDPQMQV
                     PFDDAKFAAFGFPKIIVGAVAGADQGLGTFTVEPIRGRLLTADDEGSRVTVVGSDVAR
                     ELGVAVGDSIKLRGVPFEIVGILQPTLLASDTTAMVPLAAAQELFIETLPPKLSAALV
                     AHQLASQIIVYPAAGTDAVALAGRIEASAANVSTMTSADFDQQVGSTTLIFNAIIIGV
                     AVISLAVGGLSVINTMAMSVTERTREIGIKRAIGGSRRRIIRELVAEAGVIGLIGGLI
                     GLTLGGAVVVLANEAGRSSGTLLFDLTPGTAAFAVAFSTILGMLAGIVPAWNAARLDP
                     VSALRYE"
BASE COUNT          594 a         1357 c         1405 g          535 t
ORIGIN      
        1 atgaagggag cccccgacgg ctaccccctt gcgcccgcca gccgatccgc cggggatgca
       61 gagcgatgcc gtgcgacgag gtcggcctcc gatagcgtca cgtagcggcg gaccatatcg
      121 agcgtggcgt ggccgaggat ctgctggagg ctgaacacgt cgccgccgtt gacgaggtag
      181 ccccgcgcga acgtgtggcg gaatgtatgt ggcgagcagc gcgtgccgac gcccgcccgc
      241 cggccgagcc gggcgatggc ctgctgggtg ccgcgccggc tcagtggacc ttgccgcccc
      301 gtgaagagcg ggtcggccac tgcggcccgc gcccgggtgc cgagataccg gacgagcgcc
      361 cgccgcgcag tcgttccacg cggcacgatc cgttccttcg cgcccttgcc gagaacgtgg
      421 agcgtcccgt ccgggcgcac gtcgccgacc cgcagggaca cgagctcgga cagtcggagt
      481 ccagtgtcga agaggagcag gacaagggct cgctcgcgct cgtccgccag agccagcagg
      541 ctgcggagct ccgcgtcgct gaacggggcg atgagccggc gggggacatg cggccggcgg
      601 agggcccgaa agcccggcgc tgtggcgagc tcctcggcga cacaccagtt gccgaaggcc
      661 ttcaagccgc ggacgtagcc ggcgatcgac tcgggtgcta gcgaactccg cagctctagc
      721 agccaggccc gcagctcggc cgcggtgatc gcgtcgacgg gccgaggatc accgaaccga
      781 cggaccgccc ggatgaggat catccggtac cactcgaccg tccgcggcga cgcgccttcg
      841 gccgccttcg cggcgcagaa gaagtcggcc gcggccccga ttccgatcgc cagaacgccc
      901 ccagaatcaa gcgcaatccc cgacgcgcca aaagagtcag gcgcacaagt tggggtagag
      961 tcaggcgcac ttcgcatctc cgagcctcct tggagatgcc atccgtcact ctcagaacca
     1021 catcatctgg tggtctgaac ccggtcagac cccacaggtt gtggtgttgg ctggcgagga
     1081 aggattcgaa ccttcgatct cctgatccag agtcaggcgc cttaccgctt ggccactcgc
     1141 cagtgacggg ccggatgata ccgcaggtcc gcggcccggc cgtatggccc gaacgggtgc
     1201 gtcgcgcccc ggtcggcggc cgccaacgag tcaacgcacc gcatccaccg ggaggccgac
     1261 gagatcggcg ctcacccgcc acagtcgggc ggtcgtcgcg gtgtcgtagg aggacctgtg
     1321 cgacgtcctg gccttgcggt cggcgaagta ccggccgctg accccttcga cctccggcga
     1381 gctggccagg tggacggcgg tgtccgcgcc gcgctcgggg cttcgcatga acggtcgcag
     1441 gacggcgacg aggggggcga acgcgcgggc ggggtcctcc gcgctgaagg cagtgctggt
     1501 cacgccggga tggagcacgg tcgcggtgac gccggaccct tccaatcgcc gggcgagctc
     1561 gtaggtgaac atcacgttgg caagcttcga ctggttgtag gcccgctgcc cggagtattt
     1621 ccgctcgccc atcaggtcgt cgaagtcgat ccggcccatc gactgcgccc cggacgacac
     1681 ggtgacgaca cgggccggtg cgcttgcggt gagccgctcc agcaggaggc tcgtcaacag
     1741 gaacggcgcg aggtggttga gggcgaacgt gtgctccagc ccgtcggcgg tcacatggcg
     1801 gtgggcccag aaaccgccga cgttgttgag gagcacatcg agccgcgggt aggccgagag
     1861 tacgtcgccg gccagccgcc ggacctcagc ctgtgacgac aggtcggcga cgaagacgtc
     1921 caccgcgggg ttgcccgacg cgcgcgcgat ctcggcggcc gcctcctcgg cccgaacccg
     1981 gtcgcgcccg gtgatgccga cccgggcacc catggcggcc aggctgatcg cggccgcccg
     2041 gccgatcccg ctggtgccac cggtgatcag gacggtcctg ccggtcagcg aggccgactc
     2101 agccatcacg cggccgccgc gccgaccgcc gcgccgccct cgtcggcgac cacccgctcg
     2161 tcggaaacga tgcggccgtc gcgcatccgg atgatccggt cgcacgtcgc gccgacctcg
     2221 gcgtcgtgcg tgacgagcac gacggtctgg ccgcgctcgc ggttgaaccg ccggagcagc
     2281 cccagcacct cgccggctcg cgccgagtcg aggttgccgg tcggctcgtc gccgaggagg
     2341 agcatcggct cgttgaccag cgcccgggcg atggcgacgc gctgctgctc accgccggag
     2401 agctccatcg gccgatgccc ggcccgatcg gccaggccga ccgacgccag ggccacgcgg
     2461 gcggccacgc gggcgcgacc acgggacgtc cccgcgtact cggccgcaag tgccacgttc
     2521 tccgccgcag tcagcgtcgg caccaggttg aacgactgga agatgaatcc catcgagtga
     2581 gcccggatcc gggtccgctc gcggtccggc aggcgggtgg cgtcggtgcc gtcgatcagg
     2641 agctgcggcg ccggcccgtc attccggtcg ggcgcgtgga gcagcccgag gatgtgcatc
     2701 agggtgctct tgccggagcc ggacggcccc atgatcgcga ccatctcgcc ggcggcgatg
     2761 ctgacgtcga cgccgcgcag ggcatcgatg ccggtccgcc gcgacaggcg gtaggtcttg
     2821 cggaggttgg tcgcctcgag cagggacatc gttcgctcct tcatcattcg tagcgcaggg
     2881 ccgacaccgg gtcgagccgc gcggcgttcc aggccgggac gatgccggcg agcattccga
     2941 ggatcgtgct gaaggcgacc gcgaaggcgg ccgtgccggg cgtgaggtcg aagagcaggg
     3001 ttcccgagct ccggcccgcc tcgttggcca ggacgacgac ggccccgccc agcgtcaggc
     3061 cgatgagacc gccgatcagg ccgatgacgc cggcctcggc gacgagctcg cggatgatcc
     3121 ggcgacgcga tccgccgatg gcccgcttga tgccgatctc gcgggtccgc tcggtgacgc
     3181 tcatcgccat cgtgttgatg accgacaggc cgccgaccgc gaggctgatg accgcgacgc
     3241 cgatgatgat cgcgttgaag atcaaggtgg tcgacccgac ctgctggtcg aagtcggcgc
     3301 tcgtcatcgt cgacacgttc gcggcgctcg cctcgatccg ccccgcgagc gcgaccgcat
     3361 cggttccggc cgccgggtag acgatgatct gcgaggccag ctggtgggcg acgagggccg
     3421 cgctcagctt cggcggcagg gtctcgatga agagctcctg ggccgccgcg agggggacca
     3481 tggccgtggt gtccgacgcg agcagcgtcg gctgcaggat gccgacgatc tcgaacggca
     3541 ccccgcgcag cttgatgctg tcgccgacgg ccacgccgag ctcgcgggca acatccgagc
     3601 ccacgacggt gacgcgggat ccttcgtcat cggctgtcag gagccgaccc cggatcggct
     3661 cgaccgtgaa ggtcccgagc ccttgatcag cgccggcgac ggcgccgacg atgatcttgg
     3721 ggaagccgaa ggcggcgaac ttggcgtcgt cgaacgggac ctgcatctgc gggtcgacgg
     3781 cggccacgcc ggcgatgccg cggatctggt cggcgacggc caggtcgatc ggcacgacgc
     3841 cgatcccgat gccgccgctc gaggcgtcgt tgacgatgat cttgccaacg t
//
LOCUS       DATAIW010000454         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_225610, whole genome shotgun sequence.
ACCESSION   DATAIW010000454 DATAIW010000000
VERSION     DATAIW010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225610"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VHM48_13590"
     CDS             <1..1297
                     /locus_tag="VHM48_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2756495.1"
                     /translation="NEVAMSGRPGAERRPFQPARGHRLDDALPFAGARPAALMDLPFR
                     PEDPHGAEELAGGAGFPVGRKWRSVAGQSGGRPVVLANGAEGEPLSAKDRTLLRLRPH
                     LVLDGALLAADAVGADRIALYVGSAHRDAQASIRRALGERRDLRVAIDLLDAPDSYVA
                     GEESAAVHFVNDADARPTVTPPRPYERGIGGRPTLVQNVESLACAALIGRFGDGWFRE
                     LGRDATPGSALVTISGATRDGVREIEIGTTIGELAGQAGAGDRPADRQAVLLGGYFGG
                     WLSTERSWNVALDPIALRAAGSAFGAGVVAFLGNDRCGVRATSRIMDYMAGQSAAQCG
                     PCVFGLRAIADATARLATGRPERDDLARIEHWAQQISGRGACRHPDGAAGQLLSSLRV
                     FGPEWEVHQRRRPCTRERSVVEPMRSDGRDTASLGAGAA"
     gene            1299..1625
                     /locus_tag="VHM48_13595"
     CDS             1299..1625
                     /locus_tag="VHM48_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2756496.1"
                     /translation="MAASFRAARTARVGRNDPVDVRTELVVDRIACDGFGMCAELLPE
                     LIELDDWGYPIVAAGGVPFKLIDHARRAVAVCPVLALRLARVPIAPPERGAAGWHGNR
                     PPSVRR"
BASE COUNT          206 a          674 c          632 g          230 t
ORIGIN      
        1 gaacgaggtg gccatgtcag gccgccccgg cgccgagcgg cgcccgttcc agccggcccg
       61 aggtcatcgg ctcgacgacg ccctgccgtt cgcgggtgca cggccggcgg cgctgatgga
      121 cctcccattc cggcccgaag acccgcatgg agctgaggag ctggccggcg gcgccggctt
      181 cccggtcggc cggaagtggc gctcggtcgc cggtcagtcg ggcggccggc cggtcgtcct
      241 cgccaacggc gccgagggtg agccgctcag cgccaaggat cggacgctgc tccggctccg
      301 gccgcatctc gtcctcgacg gcgccctgct ggcggccgac gccgtcggtg ccgaccggat
      361 cgcgctctac gtcgggtccg cccatcgcga tgcccaggca tcgatccggc gggccctcgg
      421 cgagcggcgc gacctgcgag tcgcgatcga tctcctcgac gcccccgact cctacgtcgc
      481 gggcgaggaa tcggcggccg tccacttcgt caacgacgcg gacgcccgcc cgaccgtcac
      541 cccgccgcgg ccctacgagc gcggaatcgg cggccggccg acgctcgtcc agaacgtcga
      601 gagcctcgcc tgcgccgccc tcatcgggcg cttcggggac ggctggttcc gcgagctcgg
      661 tcgcgacgcg acgccgggca gcgcgctcgt cacgatcagc ggcgccaccc gcgacggggt
      721 ccgcgagatc gagatcggga cgacgatcgg cgagctggcc ggccaggctg gagccggcga
      781 tcggcccgcc gatcgacagg cggtcctcct cggcggctat ttcggcggct ggctctcgac
      841 cgaacggagc tggaacgtgg cgctcgaccc gatcgccctc cgtgcggccg gcagtgcctt
      901 cggggccggc gtcgtcgcct tcctcggcaa cgaccgctgc ggcgtccggg cgacgagccg
      961 gatcatggat tacatggccg gccagtcggc ggcccagtgc gggccgtgcg tgttcggcct
     1021 ccgggcgatc gcggacgcga cggcccggct cgccacgggg cgtccggagc gcgacgacct
     1081 cgcccggatc gagcactggg cgcagcagat cagcgggcgc ggcgcctgcc gccatcccga
     1141 cggtgccgcc ggccagctcc tcagctccct gcgggtcttc gggccggaat gggaggttca
     1201 ccagcgccgg cggccctgca cccgcgaacg aagcgtcgtc gagccgatgc gctccgacgg
     1261 gcgtgacacg gcgtcgctcg gcgccggggc ggcctgacat ggcggcctcc ttccgcgccg
     1321 cgcggaccgc ccgcgtcggc aggaacgacc cggtcgacgt ccggaccgag ctcgtcgtcg
     1381 accggatcgc ctgcgacggc ttcggcatgt gcgccgagct cctcccggag ctcatcgagc
     1441 tcgacgactg gggctatccg atcgtggccg ccggcggtgt cccgttcaag ctcatcgatc
     1501 acgcgcggcg ggccgtcgcc gtctgccctg tcctggccct ccgcctcgcc cgggtcccga
     1561 tcgcgccgcc ggagcgcggc gctgctggat ggcacggcaa ccggccccca tccgtgcgac
     1621 gctgacgcca tgcggatcct cgtcgccgag gacgacccgg ggctccggga cgtcatcgtc
     1681 ctcggcctcg ccgacagcgg ctaccacgtc gatgccgtcg atcgcggtga cgacgcgatc
     1741 ga
//
LOCUS       DATAIW010000455         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_254784, whole genome shotgun sequence.
ACCESSION   DATAIW010000455 DATAIW010000000
VERSION     DATAIW010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254784"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1300
                     /locus_tag="VHM48_13600"
     CDS             302..1300
                     /locus_tag="VHM48_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2756497.1"
                     /translation="MDEERVSSKRDRLARMTRILALLNAHPDGMKPAEIARRVDSSVR
                     TVYRDLHSIEGELSMPLWSEGGRWGIEADAFLPPLKLTRSEAMAVVLSARLMVRYADK
                     YDPDLASAFEKLAAVLPAALREHVDRTLDDLSRRPTDEAFNRHVRLLTQAWAERRVVS
                     LDYAPAPYAPEAAPRAARVRPYLIEPSLQTHALYLIGWDETRTGMRTFKIERIRDVAL
                     TPESFDAPDAEIDGMFERAWDIIADQEPIEVVLRFASRVASRVREARWHPSEHVAEEA
                     DGSLTWRATVAGPIEIRLWILSWGDDVEVLAPAGLREDVAATHARAAARYDATAGT"
     gene            1300..2703
                     /locus_tag="VHM48_13605"
     CDS             1300..2703
                     /locus_tag="VHM48_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2756498.1"
                     /translation="MARHPFSAVNLISDPIHGYVELTKRLTTEESGAADLPDEDAAEE
                     DLLDTAWLQRLRRISQLQSARWVFPTAEHSRFTHGLGVMHEAGLWARQVYPSLRGALA
                     TRGEPAPSEGLVVETLRIAGLLHDVGHGPFAHFFDDHVLAAFPAPPDPRRPAGKPLTH
                     EDLSQLIIERELGELIRGLRRAPGAVPERDAFADGESIDPRWVSFIVSKPALSDATMP
                     TWVRWLQPLLSGVFTVDNLDYVRRDAWMTGVAVDVDVERLRRYTFIGERGLTLYEPGL
                     GALEMFLTSRLFLYQQVYFHRTVRAIDLDLAEVFAPSIRAIFGDRSPADALDAYVDLD
                     EYALLHRAARWTRGEELAGSPVAGDGSVTAEVAEAWRAVLLRRPHWRSEAELRLEYEA
                     DARPDEAIATLGVAEPGRVAIDLAVVDARPADASATDSLLAVEARDGSSPSLARSLGR
                     LPAYALIARRYRRIDGP"
     gene            complement(2700..3743)
                     /locus_tag="VHM48_13610"
     CDS             complement(2700..3743)
                     /locus_tag="VHM48_13610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2756499.1"
                     /translation="MPHRRHARPLRAQPPASSFLPPQPVYDRGRRIRLGQRAIVAIAI
                     GVGLALIVPLALAVLGPASGPLQPLGGGVAGAVSASREPTPTATATPLAASGPPSGLP
                     GGSGEPGGSGDVVPPPVVELSAPPIATLTGYRWPISQGRISLPFKDIPGGTRIRNGKL
                     MHDGVDMASFCGARVGAAHDGVVLAAGRHFDAQIGWIGSLAPYLHLLDAKHMWIDLPN
                     VVVIDDGNGYRSIYAHFRDVTVKVGQHVKAGQQIGHEGATGHASGCHVHYGLFSPLEA
                     KTFGVRADILMRLKLPKYEIARIDPLQVLPGGAVALKTRQIATAIAAAKTAAAKAAAA
                     KAAAAKAAAASPS"
BASE COUNT          545 a         1470 c         1417 g          591 t
ORIGIN      
        1 tcggctattc gaaacgctgc ggggaatgtg ctcgcgtgcg gcgctcgcgg atcccggcta
       61 gccgtcggcg cgcgcggccg ccgggccgtt tcgaatagcc gcatacgccc atgtcgggga
      121 gtcgatcgtc ggttccccca cgagatcgaa acattcggct attcgaaacg ccgtggggga
      181 tgcattcgga cgccgtgctc gcgggttgcc ttcgcgtccc gcgctcgcgg gttgcgttcg
      241 ccggcggcgc ccgccggacc cggcgtgaca tcgccggtcg cggcgggcga tgatgccggc
      301 gatggatgag gaacgcgtct cgagcaagcg cgaccggctg gcccggatga cgcggatcct
      361 tgccctcctc aacgcccatc cggacgggat gaagcccgcc gagatcgccc gtcgcgtcga
      421 ctcgtcggtc cggaccgtct atcgcgacct ccactcgatc gaaggcgagc tgagcatgcc
      481 cctctggtcg gagggcggcc gctggggcat cgaagccgat gccttcctgc cgccgctgaa
      541 gctgacccgg agcgaggcga tggccgtcgt cctgtcggcc cggctgatgg tccgctacgc
      601 cgacaagtac gaccccgacc tcgcgtcggc gttcgagaag ctggccgccg tcctgcccgc
      661 cgccctccgc gagcacgtcg atcggaccct cgacgacctg tcccggcgac cgaccgacga
      721 agcgttcaat cgccacgtcc ggctcctgac ccaggcctgg gccgagcgcc gcgtcgtgtc
      781 gctcgactac gcgccggcgc cctacgcacc ggaggccgcg ccgcgcgccg cccgggtgcg
      841 cccgtacctg atcgagccgt cgctccagac ccatgccctc tacctgatcg gctgggacga
      901 gacccggacc gggatgcgga ccttcaagat cgagcggatc cgcgacgtcg ccctgacgcc
      961 cgagagcttc gacgctcccg acgccgagat cgacgggatg ttcgagcggg cgtgggacat
     1021 cattgccgac caggaaccga tcgaggtcgt cctgcggttc gcatcgcggg tcgcgagccg
     1081 cgtccgcgag gcgcgctggc acccgagcga gcacgtcgcc gaggaagcgg acggctcgct
     1141 gacctggcgc gcgacggtgg ccggaccgat cgagatccgc ctctggatcc tgtcgtgggg
     1201 cgacgacgtc gaggtccttg cgccggccgg tcttcgcgag gacgtcgccg cgacccacgc
     1261 ccgggcggcc gcccgctacg acgccacggc agggacctga tggcgcgcca cccgttcagt
     1321 gccgtcaacc tgatcagcga cccgatccac ggctacgtcg agctgaccaa gcgcctcacg
     1381 accgaggagt ccggcgccgc ggacctgccc gacgaggatg ccgccgagga ggacctgctc
     1441 gataccgcct ggctccagcg actccgtcgg atcagccagc ttcagagtgc ccgctgggtg
     1501 ttcccgaccg ccgagcactc ccggttcacc cacggcctcg gcgtgatgca cgaggccggg
     1561 ctctgggctc gtcaggtcta cccctcgctg cgcggggcgc tcgcgacccg cggcgagccg
     1621 gcgccgagcg agggcctcgt tgtcgagacc ctccggatcg cgggcctgct ccacgacgtc
     1681 ggccacgggc cctttgccca tttcttcgac gatcacgtcc tcgccgcctt cccggccccg
     1741 cccgatccgc gtcggccggc cggcaagcca ctgacccacg aggacctctc gcagctgatc
     1801 atcgagcgcg agctcggcga gctgatccgc ggcctgcggc gggcgccggg tgcggtcccc
     1861 gagcgcgatg ccttcgccga cggcgagtcg atcgaccccc gctgggtctc gttcatcgtc
     1921 tcgaagcccg ccctatcgga cgcaacgatg ccgacctggg tccgctggct gcagccgctg
     1981 ctgtcgggtg tcttcaccgt cgacaacctc gactacgtcc ggcgcgacgc ctggatgacc
     2041 ggggtggcgg tcgatgtcga cgtcgagcgg ctgcggcgct acacgttcat cggcgagcgc
     2101 ggtctcacgc tgtacgagcc gggcctcggc gcgctcgaga tgttcctgac ctcgcgcctg
     2161 ttcctgtacc agcaggtcta cttccatcgg acggtccggg caatcgacct cgacctcgcc
     2221 gaagtgttcg ccccgtccat ccgggcgatc ttcggtgacc gttcgccggc cgacgcgctc
     2281 gacgcctatg tcgatctcga cgagtacgcg ctcttgcacc gagcggcgcg ctggacgcgg
     2341 ggcgaggagc tggcgggctc gccggtggcc ggcgatggct cggtcacggc cgaggtcgcc
     2401 gaggcgtggc gggcggtcct gctgcgccgg ccgcactggc gatcggaggc ggagctgcgt
     2461 ctcgaatacg aggcggacgc ccgcccggac gaggcgatcg cgacgctcgg tgtcgccgag
     2521 cctggccgcg tcgccatcga cctggccgtc gtcgatgccc gtcccgccga tgcgagtgcc
     2581 accgattcgc tgctcgcggt cgaggctcgc gacggatcgt cgccgtctct cgcccgctca
     2641 ctcggacggc tgccggccta cgcgctgatc gcccgccgtt accggcgaat cgacgggccc
     2701 tagctcgggg acgccgcggc tgccttcgcg gcggctgcct tcgcggcggc tgccttcgcg
     2761 gccgcagtct ttgccgcggc gatcgccgtg gcgatctggc gcgtcttcag ggccacggcg
     2821 ccgcccggca ggacctgcag cggatcgatc cgggcgattt cgtacttcgg cagcttcagg
     2881 cgcatgagga tgtcagcccg cacgccgaac gtcttcgcct cgagcgggct gaacagtccg
     2941 taatggacgt ggcagcccga cgcgtggccg gtcgccccct cgtggccgat ctgctggcct
     3001 gccttcacgt gctggccgac cttgacggtc acgtcccgga aatgggcgta gatcgagcga
     3061 tacccgttgc cgtcgtcgat gacgacgaca ttcggcagat caatccacat gtgcttggcg
     3121 tcgaggaggt ggaggtacgg cgcgaggctg ccgatccagc cgatctgcgc gtcgaagtgg
     3181 cgcccggcgg ccaggacgac gccgtcgtgc gcggccccga cccgggcgcc gcagaacgac
     3241 gccatgtcga ccccgtcgtg catcagcttg ccgttccgga tccgggtccc gcccgggatg
     3301 tccttgaacg gcagcgagat ccggccctgg ctgatcggcc agcgatagcc cgtcagcgtc
     3361 gcgatcggcg gtgcggacag ctcgacgaca ggcggcggca caacgtcgcc cgagccgccc
     3421 ggttcgcccg agccgcccgg cagaccggat ggcgggccgc tggcggcgag gggcgtggcc
     3481 gtcgcggtcg gcgtcggctc gcgactggcc gagaccgcgc ctgcgacgcc gccgccgagc
     3541 ggctgaagcg gtccactcgc gggacccagg acggcgaggg ccagcggaac gatgagggcc
     3601 agcccgacgc cgatggcgat cgcaacgatg gcccgctgcc ccagcctgat ccgacgaccg
     3661 cggtcgtaga ccggctgcgg cggcaggaag ctggaggccg gcggttgggc gcgcagtggc
     3721 cgggcgtgac gacggtgggg caccgcgcag gtgtatcacc cccgaagccg gcaccgcaac
     3781 ctttgcgtca ctccacaact cggccacccg gtcgcggccg cgaccgccgc gaccgccgcg
     3841 atcgcggcgc ggcagacgcg ctctcagcgg ttccgctcgg cgcggacgaa caggatcgtg
     3901 ccgatcccga tgaaggcgag gaacgtgacg gcgatcacgg cgatgttcag gaccggcggg
     3961 ctgatggcgg gagccagcag gccgggctgc gttccataga acacgttgcg ggtcgcctcc
     4021 acg
//
LOCUS       DATAIW010000456        22286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_286253, whole genome shotgun sequence.
ACCESSION   DATAIW010000456 DATAIW010000000
VERSION     DATAIW010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286253"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /gene="mazG"
                     /locus_tag="VHM48_13615"
     CDS             <1..1257
                     /gene="mazG"
                     /locus_tag="VHM48_13615"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX2756500.1"
                     /translation="LVAEARLRWGLDLVAGLQVIAAEHLVGTPLEPTRPALIVPAAIL
                     LIDTAPGAELGAAGDHPAGDDPAGLATVLPGRHGPGGDVPIAVLGRLYPADHPVGRFG
                     AAEGLTIGALSAETLATPLYVRPLAPELAVASPWAMPWISARLRLPDGCPWDREQTHE
                     SLRNHLLEEAYEVYDALGAGATPALAEELGDLLLQVVLHAQLAAEEGVFDLTDVNAAI
                     ASKIVRRHPHVFGDAEARTASDVNRQWERIKADERADAADHPAGAAGDPETRSVSAPK
                     PPKGALDGVSRILPALAASQEMQERAANIGYEWPNIEGVLDKVVEELAELRDATTAAE
                     RAEEYGDLLFVLVNVARWQGIEAEAALRAANDKFRRRFASVERQAAERRVALRDLSFA
                     ELDALWDAAKIEEKAAVAAAAKETAR"
     gene            1254..1985
                     /gene="rph"
                     /locus_tag="VHM48_13620"
     CDS             1254..1985
                     /gene="rph"
                     /locus_tag="VHM48_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX2756501.1"
                     /translation="MIGNRPQVRADGRVPTQLRPVRFELGVQKWAEGSCIVHFGDTRV
                     LCAATIADRVPPHLRGKGTGWVTAEYSMLPRATAERTDRESVKGRLGGRTHEIQRLIG
                     RSLRDAVDLTKLGERTITVDCDVLQADGGTRTASITGGYVALAAAMTTFGMDRHLVAK
                     VAAISVGLVAGEALLDLDYSEDSRADVDFNVVGTDAGTFVEVQGTAEGKPFDRAAMDG
                     LIDLASSGLERLFAAQAEILATVKR"
     gene            2155..2805
                     /locus_tag="VHM48_13625"
     CDS             2155..2805
                     /locus_tag="VHM48_13625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX2756502.1"
                     /translation="MSARPRLLVATHSAHKLAELRDLLRLERGELVSLEDLGITDDPD
                     ETGKTFATNARIKARFGTRRTGLPTLADDSGIEVDALDGGPGVRTRRFAGPAATDLDN
                     NTKLLAALDGLPPERRGARYVCVLALALPLPLPRSGEDTRAALPMRSVRGTTRGRIAA
                     APRGAGGFGYDPIFEPEGEPPGGRTFGLWSAEEKHAVSHRGRAARRMTPILRALGF"
     gene            2820..3422
                     /locus_tag="VHM48_13630"
     CDS             2820..3422
                     /locus_tag="VHM48_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2756503.1"
                     /translation="MLIIRSICVFCGASSGRDPRYAAAAAVTGETLARRGIRIVFGGG
                     RRGLMGAVADAALANGGEIIGVIPRGLVDRELAHPSLTELRVVETLHERKAQMAELAD
                     AFLAMPGGLGTLEELAEVLSWAQLDLHAKPIGLLDVGGYFGALEAFLDHAVAEGFVAE
                     RHRRLLLRDDDLDALLDRFDEWEPPAGRFERPRPVAPPRA"
     gene            complement(3564..4742)
                     /gene="galK"
                     /locus_tag="VHM48_13635"
     CDS             complement(3564..4742)
                     /gene="galK"
                     /locus_tag="VHM48_13635"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX2756504.1"
                     /translation="MTDAFTPDDLRAELVARAPAAAADPGAIRIVRAPGRVNLIGEYT
                     DVNEGLVLPAAIDREIRIAYIPSGDRRVELTRLDDDDTGAFDLDVPRSPSGTWPDYIA
                     ATAWALAGGGLPTSGLRGVIGSTLPENAGLSSSAAIELASAWALLGDAANGIDRLALA
                     RICQRAENGYVGVQSGLMDQFASSCGVEGAALLLDCRSTEWRTVAIPAGLRLVVCHTG
                     SPRRLGSSEYNIRRAQCEAAVEGIRRVDPSVRSLRDVSAALLAEARSGLDPVVARRAE
                     HVIAENDRVLATLAAFEGGDLVGIRTAFAASHASLRELFEVSSPELDLLVEIAGTVPG
                     VVAARMTGAGFGGCTVNLVHAEAVGALAAAVLATYPGRTGLSPMILPVAPADGAGILN
                     "
     gene            4778..5824
                     /locus_tag="VHM48_13640"
     CDS             4778..5824
                     /locus_tag="VHM48_13640"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEX2756505.1"
                     /translation="MTEPTGDLLDGPHRRYNPLADEWVLVSAGRTRRPWQGSREAAPA
                     DDRPRYDPDCYLCPGNGRADGSANPDYDATFVFTNDFAALRPDSEIGRVEEGLLLAEG
                     ERGTCRVVCFSPRHDLTLAMMEPADVGRVIDLWADQTAELGASYRWVQVFENRGEAMG
                     ASNPHPHGQIWAGSALPVEAAREDASQRRHVAVAGRSLLLDYVRQETTGPRVVVENDD
                     WLVVVPFWAVWPFETLIVAKVPVGRLPDVGASQRASLAPLLKELLIRYDNLFDTPFPY
                     SMGWHQAPFDADRHDHWQLHAHVYPPLLRSASVRKFMVGYELLAERQRDLTPEEAAER
                     LRAVAASRPERIRG"
     gene            complement(5946..6971)
                     /locus_tag="VHM48_13645"
     CDS             complement(5946..6971)
                     /locus_tag="VHM48_13645"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2756506.1"
                     /translation="MQLVARLHGPGDVRIHEEPLPTTGPAEVALRVISVGLCGSDLHW
                     YTDGAIGEARVDRPLILGHEIAAVIDDGPRRGTRVVLDPADPCGDCAICRGGHEELCP
                     VMRFAGHGPTDGGLRAFMSWPERLLHPVPESIGDAEASLLEALGVAIHAVDLGAVVAG
                     MRVAVVGCGPIGLLVVGALRVAGIDDIMATDLLPHRVEAARRAGAGRVWLAADQSDDP
                     TRGREPPGLEADVVFECAGDEAAVEAAIRIAAPAGRIVLVGIPGGDRTTFTASVARRK
                     GLTLVLCRRMTAADLDRAIVLAAAGRIDLSALITHRVDLEQAPEAFAALAARRGLKIV
                     VQPGRGG"
     gene            complement(7042..8307)
                     /gene="mmsB"
                     /locus_tag="VHM48_13650"
     CDS             complement(7042..8307)
                     /gene="mmsB"
                     /locus_tag="VHM48_13650"
                     /inference="COORDINATES: protein motif:HMM:NF040906.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HEX2756507.1"
                     /translation="MTQQGAPHVEIGLGSADPSRPAPTADSGERLLTVLRNNVRQYGM
                     LVALVVIIAFFQFTTGGTMLRPQNVTNVFLQNGYIIAMALGMLLIIVSGHIDLSVGSV
                     AAFTGAIAAVVMVDYKLDPILAVLAALLVGGLIGAWQGFWVAYMRIPSFIVTLSGMLL
                     FRGLTLFMLNGQPVGPFPGSFRSIASGFIPEPIGSIQDPLGPDQLKLTTLILAGLLCA
                     GLIFLDLQARREQLKYGFKVTPFGQFVARNVVLIVAIMYLAYRLSAFFGLPVVLALVL
                     VLIAVYMFLTGRTVIGRRIYAVGGNAKAATLSGVKTKQMVFLAFINMGVLSAVGGLIS
                     TARFNSATPKAGDGFELDVIAATFVGGASAYGGIGTVAGAVIGALVLGVLNIGMGLMG
                     IGIDYQFAIKALVLLGAVFVDVRGKNKDS"
     gene            complement(8304..9854)
                     /gene="mmsA"
                     /locus_tag="VHM48_13655"
     CDS             complement(8304..9854)
                     /gene="mmsA"
                     /locus_tag="VHM48_13655"
                     /inference="COORDINATES: protein motif:HMM:NF040905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2756508.1"
                     /translation="MSDVLLEMRGITKTFPGVIALDDVSLSVRRGEIHALVGENGAGK
                     STLMKVLSGVYPHGQYEGQILFEGKDAQFSDIRASEALGIVIIHQELALVPQLSITEN
                     IFLGNEQGRRGMVDWSAAHSRARDLLKMVGLDDLTDTPVRRLGVGKQQLVEIAKALSK
                     KVTLLILDEPTAALNETDSQKLLSLLRGFKAQGISSVLISHKIKEVESIADTITVIRD
                     GRVVESLDASAGVTEDRIIRGMVGRDLTHRFPDRDAKVGEVVFEVRNWNVAHPIYDDR
                     MVIKDVSLNVRAGEVVGIAGLMGAGRTEFAMSVFGRSWGRYVSGKALKNGKEIDVSSI
                     PNAIHNGIAYATEDRKTYGLVLINDVKTNITLANLEGISSRSVLSVPREIKVANEYRD
                     RLKIKCSSVKQSAVNLSGGNQQKVVLSKWLFAHPDVLILDEPTRGIDVGAKFEIYTII
                     NRLAAEGKAVIFISSELPEILGISDRIYVMAGGQIAAEMTAAEASQESIMKAIMRLGR
                     AASEGIVA"
     gene            complement(9955..11049)
                     /gene="chvE"
                     /locus_tag="VHM48_13660"
     CDS             complement(9955..11049)
                     /gene="chvE"
                     /locus_tag="VHM48_13660"
                     /inference="COORDINATES: protein motif:HMM:NF040907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2756509.1"
                     /translation="MKKLLTLAMVSMLAVAACSTPAASGGTGGSTAAGAGGLIGIAMP
                     TKSSQRWVADGDNLVKSLTALGYTTDLEYAEDKIPTQVSQVENMITKGAKLLIIAAID
                     GTTLTDTLQKAADAKIKVLAYDRLINKSPNVDFYTTFDNFKVGVLQAGSIVDCLGLKD
                     GKGPFNLELFAGSPDDNNSHFFFNGAMSILQPYLDSGKLVVQSGQKKFPDEVGTLRWD
                     GAVAQARMDNILSSKYSTARVDAVLAPYDGISRGIIASLKAVGYYSAAKPAPCVSGQD
                     AELPSVKSILAGEQTSTVFKDTRLLAAQAAKMADQMLKGGTVDVNDTKTYDNTVKIVP
                     TFLLDMVSVTKDNVQKELVDSGYYTADQVK"
     gene            complement(11308..12813)
                     /gene="araA"
                     /locus_tag="VHM48_13665"
     CDS             complement(11308..12813)
                     /gene="araA"
                     /locus_tag="VHM48_13665"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF002795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX2756510.1"
                     /translation="MLNLSSNEVWFVTGSQHLYGPETLQHVAEHAAAIAATLDDAAAI
                     PVRVVVKPVLTGPDGIRQLMGEANASPTCVGLIAWMHTFSPARMWIAGLDLLRKPLLH
                     LHTQYNREIPWADIDMDFMNLNQAAHGDREFGFIAARLRLERKVVVGHWSDPEVQERI
                     GAWTRAASARRDWETGRIARFGDNMREVAVTEGDKVEAQRRLGFAVNGYGVGDLVTRV
                     DAATDAEVDRLIEAYTDEYDVVPALRPGGDRADSLRDGARIEIGLRGFLGDGAFSAFT
                     TTFEDLHGLRQLPGLAVQRLMRDGYGFGAEGDWKTAAMVRAMKVMSVGIEGGVSFMED
                     YTYHLAASGDLILGAHMLEVCESIAGDRPRLEIHPLSIGGKDDPVRLVFDAHPGPAIA
                     ASITDLGQRFRMVATVVDVVPAEQPLPRLPVARAVWRPRPDLRTAAAAWIHAGGSHHT
                     SLGFAVTVEHLRDFAAMSGIEFLVIDETTELEAFRDRLRWNDLYHLLAKGI"
     gene            complement(12840..13484)
                     /locus_tag="VHM48_13670"
     CDS             complement(12840..13484)
                     /locus_tag="VHM48_13670"
                     /inference="COORDINATES: protein motif:HMM:NF005123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEX2756511.1"
                     /translation="MQLEDLRLELVRLHEELPRNGLVTWTGGNVSARDPDTGLVAIKP
                     SGVRYGDLTAESMVVLDIDGTIVEGELKPSSDTASHLYIYRHRPDVNGVVHTHSRYAT
                     AFAAVGRPIPVYLTAQADEFGGEIPCAGFALIGDEAIGTLVVEGIGRSPAVLLKNHGV
                     FTIGPTAAAAVKAAVMVEDIAATSWLALQIGTPDVLSDDVVEQLHRRYTTVYGQ"
     gene            complement(13486..15216)
                     /gene="araB"
                     /locus_tag="VHM48_13675"
     CDS             complement(13486..15216)
                     /gene="araB"
                     /locus_tag="VHM48_13675"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEX2756512.1"
                     /translation="MASFAIGVDFGTGSGRAILVDVADGRQLATAVSEYSHGVIDDVL
                     PLADRRVVLPPDWALQDPEDYLRVFRESIPAVLRESGVDPADVIGLGIDFTACTMLPT
                     TADGTPLALIPEFRANPHAWVKLWKHHAAQPEADRINAVAEATGQPWLDRYGGRISSE
                     WFFSKALQILDEAPAIYAAADRLLEAADWVVWRLTGVETRNACTAGYKALWSKDDGFP
                     DTSYFAALDPRLATIVDEKMSREIRFAGERAGRLTEEAAMWTGLPAGTAVAVANVDAH
                     VAVPAATVVEPGRMVMIMGTSTCHMVLSADERSVPGMCGQVRDGIIPGYVGYEAGQSA
                     VGDHFSWFVENAVPEAYRREARERGLDIYGLLEEKAARLPVGKSGLLALDWWNGNRSV
                     LVDAALGGLLVGATLATVPEEIYRALIEATAFGTRVIIETFETHGVPIREIVACGGLA
                     DKSPLTLQIYADVTGRPFRLSASEQTPALGSAMFGAVAAGVAAGGHATIEDASAAMAR
                     LQDRAHEPIAANRVVYDELYREYVRLHDYFGRGANDVMKVLKRLRADARDGGAADPSA
                     EALGATLSGA"
     gene            15404..16492
                     /locus_tag="VHM48_13680"
     CDS             15404..16492
                     /locus_tag="VHM48_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2756513.1"
                     /translation="MSRHHRITIKEIAAMCGVSTQTVSRVINKRPDVSPETRAAVEAA
                     IASVGFQPSAVARSLVQRRSQTIGVVVAGLKYFGVAQTLNGITEESEASGYALLLKEL
                     ASYDDAEVIRVVEFLLGHRVEGIIFVAPEMGTNIATVGAQLPTSYPPIVFLKSEPSAT
                     FSTIMIDNYGGARLATNHLLALGRRRIAHLAGPLAWRESRARHEGWRDALHDAGVEPG
                     PVVPGNWSSASGEVAFEQMLELAPDLDAVFAGNDQMALGIFHVAHRRGLAIPNDLAVV
                     GFDGLVEGAQFTPSLTTISQPLGELGQLAVREMLRLVASEPAQGITRSATLATELIIR
                     ESAPAAANTTTRRSAGRGQAESTPIQPR"
     gene            16519..17457
                     /locus_tag="VHM48_13685"
     CDS             16519..17457
                     /locus_tag="VHM48_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2756514.1"
                     /translation="MNTDPARGPTTRPSSVPRSGSDPRFGLFLSQANKPLSQVNDEFQ
                     MAEDLGFDHAWLVDHLIDTDGPPSHPILEAWTLLAALAARTSRIRLGVLVTSNTFRHP
                     AVLLKAAVTVDHISGGRLILGLGTGWHADEHRRYGLALPEPAERVDRFAESVELISLL
                     MSQERTTFRGDHYQLDDAPLEPRPVQKPRIPILIAAHRPRMLRLAARFADQWDTFAAM
                     PETATDGVETALADRIRYLDEACRSIGRDPAEIRRSTWTTAAALRSEDAYVDFVTRHR
                     QLGFTDFSTVMPATGDLAVLRRVAEGVIPEMRAGRL"
     gene            complement(17474..18478)
                     /locus_tag="VHM48_13690"
     CDS             complement(17474..18478)
                     /locus_tag="VHM48_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2756515.1"
                     /translation="MASARSRSTTDGELRLAMIGAGWITPAHLAALDRLGRTRLVGIA
                     SAREESARAMAEPRGAPSYVDVGRMLDEQRPDVVWVAVPPSAAIAVLDRLVERGIPFL
                     TEKPLAATDAAGPARIAAGVAERGIVAAVGYHLRALDALVEVRERLTGQPVHLVTARW
                     LSGTPAPAWWLREKVGGGQVVEQATHFYDLARSLVGEATVIGATSTREEPVVPAGADV
                     VDATAAVLRFESGAVGSFANTRRLASAVVEVELAAADLLITIRRVGDAPGSWEVVFDD
                     GRSRATVPPGRDPYEAQAAIFLDAVEAGDPTRVLSTYADALRTDRLTRAVVAATGRAG
                     "
     gene            complement(18649..20163)
                     /locus_tag="VHM48_13695"
     CDS             complement(18649..20163)
                     /locus_tag="VHM48_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2756516.1"
                     /translation="MAEPSVRDPSAPPLRARRPTAADHARPMRSQRRSIEVDQGDPTG
                     SPSSRAHGAQPAQGARPDSRPTDRRRLLAAIASSLLPGFGQAINGRLRSALLFGLPTL
                     AVIGMAWLTAQSDRPTMLIARVIAPSVLGVLLVVNVVILGWRAVAVLHAFLDRRYPLR
                     PGRLGAMGLAVLLVATAVPHLLAWSYGTAAQAMFGQIFSGSAVRSISSAPPPAEGERL
                     NVLLIGVDSAPGRTEALTDSMIVVSLDPVGRTVSMVSIPRDLASVPLGNGSIFGPKLN
                     SLMSWADRHPKDVERGGIRALEDAIGALFGIPIHYYAKVDLGGFAAMIDAVGGVDIVV
                     RKALDDPRYPGLDGKRGWSVQPGPHHFDGTDALAYARIRKSAGESDLTRAARQQEVLV
                     ALRNRAVGAGILISLPKLLHAVGATVRTDLPPDRLPELAALAEQIGGSSTMKLVLGSP
                     QIRGASSQYGSIFVPVPARIREMAKVVFGPPGADPTWPVASPGSSSAAGASAAP"
     gene            complement(20430..20957)
                     /locus_tag="VHM48_13700"
     CDS             complement(20430..20957)
                     /locus_tag="VHM48_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756517.1"
                     /translation="MTVRRRTSLRSTVVIVVIAVILGGCGPASTSARPTESFCNGVST
                     DIGGCDPIPSFVATTCDTLAAEFGTAIDAAMLDIINGPDGVAGKGRSIRIQHRGIVIT
                     TALTDRMIRLGILQNCTMPAFLDEAAAAFSSEMKSRIGAALSDGNPPASYEEFIDRLS
                     RTMSGIGTAPSFRAP"
     gene            complement(20996..21541)
                     /locus_tag="VHM48_13705"
     CDS             complement(20996..21541)
                     /locus_tag="VHM48_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756518.1"
                     /translation="MFALLILPTGVLAVDGEIGDLGNRNSITELAVTADGRPTTRISY
                     PHDCPAGISTCWIETQFRYKCPEFWCFSVNSQPWKLLPSSGTALGDCIGADDNYWEVW
                     YRVGFAATGTKTVTYSGELEFYMNAKGSFTYKNIAEAELNLTDKSGTRGSVTLETVTA
                     KSDVMDPVLVTTSGGRTFHTC"
     gene            21927..>22286
                     /locus_tag="VHM48_13710"
     CDS             21927..>22286
                     /locus_tag="VHM48_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756519.1"
                     /translation="MRAFMTRLDPRRAPAVVLLLAPPVLLVLIVGLTPEPTRLIFAGD
                     LSVYRRYGLQVLGGSIPYLDFHVEYPPLALVPMTLPLLALPPGGIDDLGYIWRFTLAQ
                     GALAAAVGWLLFEATGRS"
BASE COUNT         3365 a         7726 c         7824 g         3371 t
ORIGIN      
        1 ctcgtcgccg aggcacgtct ccgctggggc ctcgaccttg ttgccgggct ccaggtcatc
       61 gccgccgagc acctggtcgg aacgccgctc gagccgacgc gcccggccct gatcgtgccg
      121 gctgcgatcc ttctcatcga cacggcgccc ggcgcggagc tcggcgcggc cggagaccac
      181 ccggccggag acgacccggc cggccttgcc accgttctgc cgggtcgtca cgggcccggc
      241 ggtgatgttc cgatcgccgt gctcggccgg ctctatccgg ccgatcatcc ggtcgggcgg
      301 ttcggcgcgg ccgaagggct gaccatcggc gcgctcagcg ccgaaacgct ggcgacgccg
      361 ctgtacgtcc ggccactggc ccccgagctg gctgtcgcga gcccctgggc gatgccctgg
      421 atctccgccc ggctgcggct tcctgatggt tgcccgtggg atcgcgagca gacgcacgag
      481 tcgctccgga accacctcct cgaggaggcg tacgaggtct acgacgccct cggcgccggg
      541 gccacgccgg ccctcgccga ggaactcggg gatctgctcc tccaggtcgt gctccatgcc
      601 cagctcgcgg ccgaggaggg cgtgttcgac ctgaccgacg tcaacgccgc gatcgcgtcg
      661 aagatcgtcc gccgccatcc gcacgtgttc ggcgacgccg aggcccgcac ggcgagcgac
      721 gtgaatcgcc agtgggaacg gatcaaggcc gacgagcggg ccgacgccgc ggaccatccg
      781 gccggcgccg ccggcgaccc tgagactcga tcggtatccg ctccgaagcc gccgaagggc
      841 gccctcgacg gcgtgagccg gatcctgcca gccctcgccg ccagccagga gatgcaggag
      901 cgggcggcca acatcggcta cgaatggccg aacatcgaag gggtcctcga caaggtcgtc
      961 gaggagctcg ccgagctgcg cgacgccacc accgccgccg agcgggctga ggaatacggc
     1021 gatctcctgt tcgtcctcgt caacgtcgcc cgctggcagg ggatcgaggc cgaggccgcc
     1081 ctccgcgccg ccaacgacaa gttccgccgc cgcttcgcca gtgtcgagcg ccaggccgcc
     1141 gagcggaggg tcgcgctgcg cgacctttcg ttcgccgagc tcgacgccct ctgggatgcg
     1201 gccaagatcg aagagaaggc ggccgtcgcg gccgcagcga aggagaccgc ccgatgatcg
     1261 gcaaccgccc ccaggtccgc gccgacggcc gggtcccgac ccagctccgc cccgtccgct
     1321 tcgagctcgg cgtccagaag tgggccgagg gctcgtgcat cgtccatttc ggcgacaccc
     1381 gggtcctgtg cgcggcgacg atcgccgacc gcgtgccgcc ccacctccgc ggcaagggca
     1441 ccggctgggt gaccgccgag tactcgatgc tgccgcgcgc caccgccgag cggaccgatc
     1501 gcgagtcggt caagggcagg ctcggcggcc ggacgcacga gatccagcgg ctgatcgggc
     1561 ggtccctgcg cgacgcggtc gatctgacca agctgggcga gcggacgatc accgtcgact
     1621 gcgacgtcct ccaggccgac ggcgggaccc ggacggcctc gatcacgggc ggctacgtgg
     1681 cgctcgccgc ggcgatgacg accttcggga tggaccgcca cctggtcgcg aaggtcgcgg
     1741 caatcagcgt cgggctcgtc gccggcgagg cgctcctcga cctcgactac agcgaggact
     1801 cgcgggccga cgtcgacttc aacgtcgtcg gcactgacgc cggcacgttc gtcgaggtcc
     1861 agggcactgc cgagggcaag ccgttcgacc gggcggccat ggatgggctg atcgacctcg
     1921 cgtcgtccgg gctcgagcgg ctgttcgcgg cccaggccga gatcctggcc accgtcaagc
     1981 ggtgacgccg cccgctccgg tcccggccgc acgctgcgca tccagcgcgc actgcggcga
     2041 acgtcccgcc tggatcgccg tcatccgggg ggccccttcg tcgtgttgct cgcgtcgtgc
     2101 gcattccgcc cctggggccg ccgtgccttc gacgcaacgc gcacctggtg cgcagtgagc
     2161 gccaggccgc ggctcctggt cgccacccac tcggcccaca agctggccga gctgcgcgac
     2221 ctcctccggc tggagcgagg cgagctcgtc agcctcgagg accttggcat caccgacgac
     2281 cccgacgaga ccggcaagac gttcgcgacg aacgcccgga tcaaggcccg cttcggcacg
     2341 aggcggactg gtctgccgac gctcgccgac gactcgggga tcgaagtcga tgcgctcgac
     2401 ggcggcccgg gcgtcaggac acggcgcttt gccggacccg ccgcgaccga cctcgacaac
     2461 aacacgaagc tgctggcggc tctcgacggc ctcccgccag agcggcgcgg cgcgcgctac
     2521 gtctgcgtgc tcgcgctggc gttgccgctg ccgctgccgc gatcgggcga ggacacccgg
     2581 gccgcactgc cgatgcgttc cgtccggggc acgacccggg gacggatcgc ggccgctccc
     2641 cgcggcgcgg gcggcttcgg ctacgacccg atcttcgagc ccgagggcga gccgcccggc
     2701 ggtcgcacct tcgggctgtg gtcagccgag gagaagcacg ccgtctcgca tcgcggtcgg
     2761 gcggcccggc ggatgacgcc gatcctgcgc gcgctcgggt tctaggggac gaggacgcga
     2821 tgctgatcat ccgctcgatc tgcgtgttct gcggggcgtc gagcggccgc gatccgcgct
     2881 acgcggccgc cgcggccgtc accggggaga cccttgcccg gcgcgggatc cgcatcgttt
     2941 tcggcggcgg tcgccgcggg ctgatgggcg cggtcgccga tgccgcgctg gccaacggcg
     3001 gcgagatcat cggggtgatc ccgcgcgggc tcgtcgaccg tgagctcgcc cacccgtcgc
     3061 tgaccgagct ccgcgtcgtc gagaccctcc acgagcggaa ggcacagatg gccgagctgg
     3121 ccgacgcgtt cctggccatg cccggcggcc tcggcacgct cgaggaactg gccgaggtcc
     3181 tctcgtgggc ccagctcgac ctccacgcca agccgatcgg gctgctcgac gtcggcggct
     3241 acttcggcgc gctggaggca ttcctcgacc acgcggtcgc cgaggggttc gtcgccgagc
     3301 gccaccgccg gctgctcctc cgcgacgacg atctcgacgc gctgctcgac cggttcgacg
     3361 aatgggagcc gcccgccggc cgcttcgagc gtcctcggcc ggtcgcgccg ccgcgcgcct
     3421 gagcggccgc cggctctcgg gggtctccgt cggcggacat ggtgacgccg ggcgatcgat
     3481 cagcctgcca gtgatcgccc ggcgtcgtgt ctgtgacgca tgagccgcgc caaccgttcc
     3541 gcgacgccgg atgttcgtcg atctcagttc aggattccgg cgccgtcggc cggcgccacc
     3601 ggcaggatca tcgggctgag cccggtccgc ccggggtacg tggcgaggac ggcggccgca
     3661 agtgccccga ccgcctcggc gtggacgagg ttgaccgtgc agccgccgaa ccccgcgccc
     3721 gtcatccggg ccgcgacgac gcccgggacc gtgccggcga tctcgacgag caagtcgagc
     3781 tccggtgagc tgacctcgaa caactcgcgc agcgaggcgt gactcgccgc gaacgccgtg
     3841 cggatgccga cgaggtcgcc gccctcgaac gccgcgagcg tcgcgaggac ccggtcattc
     3901 tcggcgatga catgctcggc gcggcgggcg acgaccggat cgagcccgct ccgcgcctcg
     3961 gcgaggaggg ccgcgctcac gtcgcgcagc gatcgcaccg acgggtccac ccggcgaatg
     4021 ccttcgactg ccgcttcaca ttgggcccgc cggatgttgt actcggagct gcccagtcgg
     4081 cgtggcgagc cggtgtggca cacgaccagg cgaaggcccg ccgggatggc gaccgtccgc
     4141 cactcggtcg accggcagtc caacaggagg gcggccccct cgacgccgca cgacgacgcg
     4201 aactggtcca tgagtccgga ttgcacgccg acgtagccgt tctcggcccg ctggcagatc
     4261 cgggcgaggg cgaggcgatc gatcccgttc gcagcgtcgc cgagaagcgc ccaggccgat
     4321 gccagctcga tcgcagccga cgacgacagc ccggcgttct ccggcagggt cgagccgatg
     4381 acgcctcgca gcccactggt cggcagaccg cccccggcca gggcccacgc cgtcgccgcg
     4441 atgtagtccg gccacgtgcc ggagggtgag cgcgggacgt cgaggtcgaa tgcaccggta
     4501 tcgtcgtcgt cgagccgggt cagctcgacg cgccgatcgc cgctcgggat gtaggcgatc
     4561 cggatctccc ggtcgatggc ggccggcagg acgagcccct cgttgacgtc cgtgtactcg
     4621 ccgatgaggt tcacccggcc gggcgcccgg acaatgcgga tggccccggg gtcggcggcg
     4681 gccgcgggcg cgcgggcaac cagctccgcg cggaggtcgt ccggagtgaa tgcgtcggtc
     4741 atcgaggctt gccccagctg acgtatgctc gcgagcgatg accgaaccga ccggggacct
     4801 cctcgacgga ccccaccgcc gctacaaccc gctcgccgac gagtgggttc tcgtgtcggc
     4861 cgggcgcacc cgacgaccat ggcaggggtc ccgcgaggca gcgcccgcgg acgaccggcc
     4921 gcggtacgac cccgattgtt atctgtgtcc cggcaacggt cgggccgacg gatcggcgaa
     4981 cccggactat gacgccacgt tcgtcttcac caacgacttc gcggcgcttc ggcccgacag
     5041 cgagatcggc cgcgtcgagg agggcctgct ccttgccgag ggcgaacgtg ggacctgccg
     5101 ggtggtctgc ttctcgcctc gccacgacct gaccctggcc atgatggagc cggccgatgt
     5161 cggtcgggtc atcgacctgt gggcagatca gaccgccgag ctcggagcga gctatcgctg
     5221 ggtccaggtc ttcgagaacc gcggcgaggc gatgggcgcg tcgaaccccc acccccacgg
     5281 tcagatctgg gcggggtccg ccctgccggt cgaggccgca cgcgaggatg ccagccagcg
     5341 ccgccacgtc gcggtcgcgg ggcgctcgct gctgctggac tacgtccgcc aggagacgac
     5401 gggcccgagg gtcgtcgtgg agaacgacga ctggctggtg gtcgtcccgt tctgggcggt
     5461 ctggccattc gagaccctga tcgtggcgaa ggtcccggtc gggcgcctgc cggatgtcgg
     5521 cgcgtcgcag cgcgcgagcc tcgctccgct cctcaaggag ctgctgatcc gctacgacaa
     5581 tctgttcgat acgccgttcc cgtattcgat gggctggcac caggcgccgt tcgacgcgga
     5641 tcgccacgac cactggcagc tccacgccca cgtctatccg cccctgctgc ggtcggccag
     5701 cgtccgcaag ttcatggtcg gctacgagct ccttgcggag cgtcagcgcg acctgacccc
     5761 ggaggaggcc gccgaacggc tgcgggccgt cgcggcttct cgcccggaga ggatccgggg
     5821 ctgactccga tgtcacggcc caccatccgg ccccgcaggc cgtccgcccg cggatggccg
     5881 gatctggagt cgggcattgc cgcattccag gacaagccgg ccgtccgcgg ccggtcccgc
     5941 gcggctcatc cgccgcgacc gggctgaacg acgatcttca ggccacgcct cgccgcgagg
     6001 gcggcgaacg cctcgggcgc ctgttcgagg tcgacgcgat gggtgatcaa cgccgacagg
     6061 tcgatccggc ccgcagcggc gagaacgatc gctcgatcga gatctgcggc ggtcatccgg
     6121 cgacagagca cgagggtgag gcccttccgc cgcgcgaccg aggccgtgaa cgtcgtccgg
     6181 tcgccacccg ggatcccgac gaggacgatg cgtccggccg gcgccgcgat ccggatcgcc
     6241 gcctcgacgg ccgcttcgtc cccggcgcac tcgaagacga cgtcggcctc gaggcccggc
     6301 ggttcgcggc ctcgagtcgg atcgtcactc tgatctgcgg cgagccagac ccggcccgcc
     6361 cctgcgcgtc gcgcggcctc gacccggtgg gggagcagat cggtggccat gatgtcgtcg
     6421 atcccggcca cgcgaagggc gccgacgacg agcagtccga tcgggccgca gccgacgacc
     6481 gctacccgca tcccggcgac gaccgccccg agatcgaccg cgtggatcgc cacgccgagc
     6541 gcctcgagaa ggctcgcctc cgcgtcgccg atcgactccg gaacggggtg gagaagccgt
     6601 tccggccagc tcatgaaggc gcgcaggccg ccgtccgtcg gtccgtgacc cgcgaatcgc
     6661 atgaccggac agagctcttc gtggccgcct cgacagattg cgcagtcgcc gcatggatcg
     6721 gccggatcga gcacgacccg tgttccgcgc cgtggaccgt cgtcgatcac tgccgcgatc
     6781 tcatgaccga ggatgagcgg ccggtcgaca cgcgcctcac cgatcgcccc atcggtgtac
     6841 cagtggaggt cggagccgca cagcccgacc gagatgaccc gcagggcgac ctcggccggc
     6901 ccggtcgtcg ggagcggctc ctcgtggatc cggacgtcgc cgggcccgtg gaggcgggcg
     6961 acgagctgca ccgacggcct cctcagcagg tcgctcacgg gccgggccgt ggaggaggcc
     7021 cggcccggcc cgcgcgacag atcaggagtc cttgttcttg ccccggacgt cgacgaacac
     7081 ggcgccgagg aggacgaggg ccttgatcgc gaactggtag tcgatcccga tccccatcag
     7141 gcccatgccg atgttcagga ccccgaggac gagcgcgccg atgacggcgc cggcgaccgt
     7201 cccgatcccg ccatacgccg acgcaccgcc gacgaaggtc gcggcgatga cgtcgagctc
     7261 gaagccatcg ccggcctttg gagtcgccga gttgaatcga gcggtcgaga tcaggcctcc
     7321 gaccgccgac aggacaccca tgttgatgaa cgcaaggaag accatctgct tggtcttgac
     7381 cccggacagc gtcgccgcct tggcgttgcc gccgaccgcg tagatccgcc ggccgatgac
     7441 ggtccgcccg gtcaggaaca tgtacaccgc gatcaggacc agcacgagag cgaggacgac
     7501 cggcaggccg aagaacgccg acagccgata ggcgagatac atgatggcga cgatcaggac
     7561 gacgttccgg gcgacgaact ggccaaaggg cgtgaccttg aagccgtact tgagctgctc
     7621 tcgccgcgcc tgcaggtcga ggaagatcag gccggcgcag agcaggcccg ccaggatcag
     7681 ggtggtcagc ttcagctggt ccggtccgag cggatcctgg atcgagccga tcggttcggg
     7741 gatgaacccg gaggcgatcg accggaagct ccccgggaat ggcccgacag gctggccgtt
     7801 gagcatgaac agcgtcaggc cgcggaacag gagcatcccg gacagggtca cgatgaagga
     7861 cgggatccgc atgtaggcga cccagaaccc ctgccacgcg ccgatgagcc cgccgaccag
     7921 cagggcggcc aggaccgcga ggatcggatc cagcttgtag tcgaccatca cgacggccgc
     7981 gatcgcgccg gtgaatgccg ccacggaccc gaccgagagg tcgatgtgcc cggagacgat
     8041 gatcaacagc atccccaggg ccatcgcgat gatgtagccg ttctgaagga agacgttggt
     8101 cacgttttgc ggccggagca tcgtcccgcc ggtcgtgaac tggaagaacg cgatgatcac
     8161 gaccagggcg acgagcatcc cgtactggcg cacgttgttg cgcaggacgg tcagaaggcg
     8221 ctcgccactg tcggcggtcg gcgcaggcct ggaagggtcg gccgaaccaa gtccgatctc
     8281 gacgtgcgga gcgccttgct gggtcatgcg acgatcccct ccgaggcggc ccggccgagc
     8341 cgcatgatgg ccttcatgat cgactcctgg gaagcctcgg cggccgtcat ctcggcagcg
     8401 atctggccgc cggccatgac gtagatccgg tccgagatgc cgaggatctc cggcagttct
     8461 gacgagatga agatcaccgc cttgccctcc gctgcgaggc gattgatgat cgtgtagatc
     8521 tcgaacttgg caccgacgtc gatcccgcgg gtcggctcat ccaggatgag gacgtccgga
     8581 tgagcaaaca gccacttcga caggacgacc ttctgctggt tgccaccgga caggttgacg
     8641 gcggactgct tgacgctcga gcacttgatc ttcagtcggt cgcggtattc gttggcgacc
     8701 ttgatctcgc gcggcacgct caggaccgat cgggaggaaa tgccttcgag gttggccagg
     8761 gtgatgttcg tcttgacgtc atttatcagg acgagcccgt atgtcttgcg atcttccgtc
     8821 gcgtaggcga tcccgttgtg aatggcgttc gggatcgagc tgacgtcgat ctccttgccg
     8881 ttcttgaggg ccttcccgct gacatatcgt ccccacgatc gcccgaagac gctcatcgcg
     8941 aactcggtcc gcccggctcc catcaggccg gcgatgccca cgacttcgcc ggcgcggacg
     9001 ttgaggctga cgtccttgat caccatccga tcgtcataga tcggatgagc gacgttccag
     9061 ttgcggacct cgaagacgac ctcgccgacc ttggcatcgc gatccgggaa gcggtgggtc
     9121 aggtcccgcc cgaccatccc gcggatgatt cgatcctcgg tcacgccggc cgacgcgtcc
     9181 agcgattcga cgacgcgtcc gtcgcgaatc accgtgatcg tatcggcgat cgactcgact
     9241 tccttgatct tgtgagagat cagcacggac gaaatgccct gggccttgaa gccacgcagc
     9301 aggctgagca gcttctggct gtccgtctcg ttgagcgcgg ccgtcggctc gtccaggatg
     9361 aggagggtga ccttcttgga gagggccttg gcgatctcga cgagttgctg cttgccgacg
     9421 ccgagccgtc tgacgggcgt gtcggtgagg tcgtcgagac cgaccatctt gagcaggtcg
     9481 cgcgcccggg aatgggctgc gctccagtcg accatgccac ggcggccctg ttcgttcccg
     9541 aggaagatgt tctcggtgat cgagagctgg ggcaccaggg cgagctcctg gtgaatgatg
     9601 acgatgccga gggcctcgct ggcccggatg tcggagaact gggcgtcctt gccctcgaac
     9661 aggatctgac cctcgtactg gccgtgcgga tagacgccgc tcaggacctt catcagggtc
     9721 gacttgccgg ctccattctc gcccacgagg gcatggatct cgccgcggcg gacggacagg
     9781 ctgacgtcgt cgagggcgat gacccccgga aacgttttcg tgatcccgcg catctcgagg
     9841 agtacgtcgc tcactcggac agctgcccct tcatcagtgc ttcagggaaa tggaacgcgg
     9901 gtgtgcgtgg tcgtcgaccg ccgcacaccc gcagggtaac cgagttgctg tcgcctactt
     9961 gacctggtcc gcggtgtagt acccgctgtc aaccagttcc ttctggacgt tgtccttggt
    10021 cacgctgacc atgtcgagaa ggaaggtcgg aacgatcttg acggtgttgt cgtaggtctt
    10081 ggtgtcgttc acgtcgaccg tcccgccctt gagcatctgg tcagccatct tggcggcctg
    10141 ggcagccagg agacgggtgt ccttgaagac cgtggaagtc tgctcaccgg cgaggatcga
    10201 cttgaccgac gggagctccg cgtcctggcc gctgacgcac ggcgcgggct tggcggcgga
    10261 gtagtagccg acggccttga gcgaggcgat gatgccccga ctgatgccgt catacggagc
    10321 gaggaccgcg tcgacacggg cggtcgagta cttcgagctc aggatgttgt ccatccgggc
    10381 ctgcgcgacg gcgccgtccc agcggagagt gccgacctcg tcagggaact tcttctggcc
    10441 actctggacg accagcttgc cgctgtcgag gtagggctgg aggatcgaca tcgcgccatt
    10501 gaagaagaag tgcgagttgt tgtcgtccgg cgagccggcg aacagctcga ggttgaacgg
    10561 acccttgccg tccttgaggc cgaggcagtc gacgatcgag ccggcctgga ggacgccgac
    10621 cttgaagttg tcgaaggtcg tgtagaagtc gacgttcggc gacttgttga tcaggcggtc
    10681 gtaggcgagg accttgatct tcgcgtcggc ggccttctgg agggtgtccg tcagggtcgt
    10741 cccgtcgatc gcggcgatga tcaggagctt ggcgcccttc gtgatcatgt tctcgacctg
    10801 ggagacctgg gtcgggatct tgtcctcggc gtactcgagg tcggtcgtgt agcccagtgc
    10861 ggtcagcgac ttgacgaggt tgtcgccgtc ggcgacccag cgctgggagg acttggtcgg
    10921 catggcgatc ccgatcagcc cgccggcgcc ggcagccgtc gagccgccgg tgccgccgct
    10981 cgcggccggt gtgctgcagg ccgccacggc aagcatgctg accatcgcga gggtgagaag
    11041 tttcttcatc ggtgcgctgc gctcctccaa atggcggggc agaccgcccc tggtccttcg
    11101 gccggcgtct ggccgtggag gcgaggtcac ccgcgctggc gggccttgtg atccctgccg
    11161 catccacgct ctcaggagca cagccgttat cgttcccgtg aacgataacg ctcgcatcct
    11221 agtctcgccg tcgtcggccg tcaagccgat ttgtcaatgg ctgggccgat aacgttcggt
    11281 cacgaaacgg agatggccga ggaccgatca gatgcccttg gcgaggaggt gatagaggtc
    11341 gttccagcga aggcggtcgc ggaatgcctc gagctccgtc gtctcgtcga tcaccaggaa
    11401 ctcgatcccg gacatcgccg cgaagtcccg aagatgctcg accgtgaccg cgaacccgag
    11461 actcgtgtga tgggagccgc cggcatggat ccaggcggcg gcagcggtcc ggaggtccgg
    11521 tcgtggtcgc cagactgccc gggcaacggg caggcgcggc agcggctgtt cggccgggac
    11581 gacgtcgacg accgtcgcga ccatccggaa ccgctggccc agatcggtga tcgaggccgc
    11641 gatcgccggc ccgggatggg cgtcgaagac caggcggacc gggtcgtcct tgccgccgat
    11701 cgagagggga tggatctcga gacgcggccg atccccggcg atcgactcgc agacctcgag
    11761 catgtgggcg ccgaggatga ggtcgcccga cgccgcgagg tggtaggtgt agtcctccat
    11821 gaacgacacg ccgccctcga tcccgacgct catgaccttc atcgcccgga ccatcgccgc
    11881 ggtcttccag tcgccctctg ccccgaaccc gtagccgtcg cgcatcagcc gctggaccgc
    11941 cagtcccggc agctggcgga ggccgtggag gtcctcgaat gtcgtcgtga atgcggagaa
    12001 cgcgccgtcg ccgaggaagc cccggagacc gatctcgatc cgggcgccat cgcgcaacga
    12061 gtccgcccgg tcgccgcccg gccggagggc cgggacgacg tcgtattcgt cggtatacgc
    12121 ctcgatcaac cggtcgacct ccgcgtcggt cgccgcgtcg acacgagtca cgaggtcgcc
    12181 gacgccgtag ccgttgacgg cgaagccgag ccgtcgctgg gcttcgacct tgtcgccctc
    12241 cgtgaccgcg acctcgcgca tgttgtcgcc gaaacgggcg atccggccgg tctcccagtc
    12301 acgcctggcc gaggcggccc gggtccaggc cccgatccgt tcctgcacct cgggatcgga
    12361 ccaatggccg acgaccacct tccgctcgag tcgcaggcgg gcggcgatga acccgaactc
    12421 acggtcgccg tgggcggcct ggttcaggtt catgaagtcc atgtcgatgt ctgcccacgg
    12481 gatctcgcgg ttgtactggg tgtggaggtg gagcagcggc ttgcggagaa ggtccaggcc
    12541 ggcgatccac atcctggccg gcgagaaggt gtgcatccag gcgatgagcc cgacgcacgt
    12601 cgggctggcg ttggcctcgc ccatcagctg gcggatcccg tcgggcccgg tcaggaccgg
    12661 cttgacgacg acgcggacgg ggatcgccgc ggcatcgtcg agggtcgccg cgatcgcggc
    12721 agcatgctcg gcaacgtgct ggagcgtctc cgggccatag aggtgctggc tgccggtgac
    12781 gaaccacacc tcgttcgacg agagattgag cacgagagga ctcctcgatc tggcggcggc
    12841 tactgtccgt agacggtcgt gtagcgacga tggagctgct caacgacgtc gtcggacagg
    12901 acgtcgggcg tgccgatctg gagggccagc caggaggtgg ccgcgatgtc ctcgaccatg
    12961 accgcggcct tgaccgccgc ggcggcggtc ggaccgatcg tgaagacgcc gtggttcttg
    13021 aggagcaccg ccggcgaccg tccgatccct tcgacgacca gggtgccgat cgcctcgtcg
    13081 ccgatcaggg cgaagccggc gcacggaatc tcgcccccga actcgtcggc ctgggccgtc
    13141 aggtagaccg ggatcggccg gcccactgcg gcgaacgcgg tcgcatagcg cgaatgcgta
    13201 tggaccacgc cgttgacgtc gggccggtgg cgatagatgt agaggtggct cgccgtatcc
    13261 gacgacggtt tgagctcgcc ctcgacgatc gttccgtcga tgtcgaggac gaccatgctc
    13321 tcggcggtca ggtcgccgta gcggacgccg gacggcttga tcgcgacgag ccccgtatcc
    13381 gggtcccgag cgctgacgtt cccgccggtc cacgtgacga gcccgttgcg gggcagctcc
    13441 tcgtgcagcc ggacgagctc gaggcggagg tcttcgagtt gcatctcaag ctcccgagag
    13501 tgtcgcacca agggcctcag cggacgggtc ggccgccccg ccatcgcgcg cgtcggcgcg
    13561 gagacgcttc agcaccttca tgacgtcgtt ggcgccccgc ccgaaatagt cgtggagacg
    13621 gacgtattcg cgatacagct cgtcgtagac cacgcggttg gccgcgatcg gctcgtgggc
    13681 gcggtcctgc agacgggcca tcgccgcgga cgcgtcctcg atggtcgcgt ggccgcccgc
    13741 cgcgacgccg gccgccaccg ccccgaacat tgccgagccg agtgccggcg tctgttcgga
    13801 cgcgctcagc cggaacggcc ggcccgtgac gtccgcgtag atctggagcg tgagcgggct
    13861 cttgtcggcc aatccgccgc aggcgacgat ctcgcggatc ggcacgccat gggtctcgaa
    13921 cgtttcgatg atcacccggg tcccgaaggc ggtcgcttcg atcagggctc ggtagatctc
    13981 ctccggcacg gtcgcgaggg tcgccccgac gaggaggccg ccgagggcgg cgtcgacgag
    14041 gactgaccgg ttgccgttcc accagtcgag ggccaggaga ccggacttgc cgaccggcag
    14101 gcgggcagcc ttctcctcga ggagaccgta gatgtcgagg ccgcgttcgc gggcctcccg
    14161 ccggtaggcc tcgggaacgg cgttttcgac aaaccagctg aaatggtcgc cgacggccga
    14221 ctgacccgcc tcgtacccga catagcccgg gatgatccca tcccgaacct gaccgcacat
    14281 cccggggacg gaccgctcgt cggccgagag gaccatgtga caggtcgagg tgcccatgat
    14341 catcaccatc cggcccggct cgacgaccgt cgcggcaggc accgcgacat gggcatcgac
    14401 gttggcgacg gcgaccgccg tccctgcggg caggcccgtc cacatggccg cttcctcggt
    14461 cagacgtccg gcgcgctcgc cggcaaatcg gatctcgcgg ctcatcttct catcgacgat
    14521 cgtggcaagg cgaggatcga gcgccgcgaa gtagctcgtg tcgggaaagc cgtcgtcctt
    14581 cgaccacagc gccttgtagc cggccgtgca ggcgttccgg gtctcgactc cggtcaggcg
    14641 ccagacgacc cagtcggcgg cttcgagcag gcggtcggcg gcagcgtaga tcgcgggtgc
    14701 ctcgtcgagg atctggagcg ccttcgagaa gaaccactcg gacgagatcc ggccaccgta
    14761 gcgatcgagc cagggctggc cggtcgcctc ggcgacggcg ttgatccgat cggcctcggg
    14821 ctgggcagcg tggtgcttcc agagcttgac ccaggcatgc gggttggcgc ggaactccgg
    14881 gatcagggcc agcggcgtgc cgtcggcggt cgtcggcagc atcgtgcagg ccgtgaagtc
    14941 gatccccaga ccgatcacgt ccgccggatc gacgcccgac tcgcggagga cggccgggat
    15001 cgactcccgg aagacgcgga ggtagtcttc cgggtcctgg agcgcccagt ccggggggag
    15061 gacgacccgt cgatcggcca gcggcagcac atcgtcgatg acgccgtgcg aatattcaga
    15121 gaccgccgtg gcgagctgcc ggccgtcggc cacgtcgacc aggatcgctc ggccggatcc
    15181 ggtcccgaaa tcgacgccga tcgcgaagct ggccacgctg cctccctgtc ctgccggccg
    15241 gtcgccaccc ggtccgcgcc aggcccgggc cgaagccggg tggccggcgg ggttgatcgg
    15301 cggacgacgt atgttagcgt tcccgctaac gttcacggcg tagaataacg tagcagtcgc
    15361 ggacgctccg cacaacccgc caaccccgtc cgaggtcgct ggattgtccc gtcaccaccg
    15421 catcacgatc aaggagatcg ccgcgatgtg cggagtctcc acccagaccg tctcgcgcgt
    15481 gatcaacaag cgcccggacg tctcgccgga gacccgcgcg gccgtcgagg ccgcgatcgc
    15541 ctcggtcggc ttccagccca gcgccgtcgc ccggagcctc gtccagcgcc gctcgcagac
    15601 gatcggcgtc gtggttgccg gcctcaagta cttcggcgtc gcccagaccc tcaacggcat
    15661 caccgaggag agcgaggcat cgggctacgc cctcctgctg aaggagctgg ccagctacga
    15721 cgacgccgag gtgatccggg tcgtcgagtt cctgctgggc catcgggtcg aagggatcat
    15781 cttcgtcgct ccggagatgg ggacgaacat cgcgaccgtc ggggcgcagc tgccgacgtc
    15841 gtacccgccg atcgtcttcc tcaagtccga gccgtccgcc acgttctcca cgatcatgat
    15901 cgacaactac ggcggcgccc ggctggccac gaaccacctt ctggccctcg gccggcggcg
    15961 gatcgcccat ctcgccgggc cgctcgcgtg gcgcgagtcg cgggcgcgtc acgagggttg
    16021 gcgagacgcc ctccacgatg ccggtgtgga gcccggtccg gtcgttcccg gcaactggtc
    16081 ctccgccagc ggtgaggtcg cctttgaaca gatgctcgag ctcgcgccgg acctcgacgc
    16141 cgtgttcgcc ggcaatgacc agatggcgct cggcatcttc cacgtggcgc atcgccgcgg
    16201 actggcgatc ccgaacgacc ttgcggtcgt cggcttcgac ggcctggtcg agggagccca
    16261 gttcacgccg tcgttgacga cgatcagcca gccactcggg gaactcggcc agctcgccgt
    16321 gcgcgagatg ctccggctcg tggcgagtga gccggcccag ggaatcaccc gcagcgcgac
    16381 cctcgccacg gagctcatca tccgtgagag tgccccagcc gcggccaata cgacgactcg
    16441 ccggagcgcc ggacggggcc aggcggagtc gaccccgatt cagccgcgct gacgtccggt
    16501 gttgggaggt cggaggtcgt gaacacggac ccggcgcgtg ggccgaccac gcggccctcg
    16561 tcggtcccgc ggtctgggtc ggacccgcgg ttcgggctgt tcctcagcca ggcgaacaag
    16621 ccgctgagcc aggtcaacga cgagttccag atggcggagg acctcggctt cgatcatgcc
    16681 tggctcgtcg atcacctcat cgataccgac ggcccaccga gccatccgat cctcgaggcc
    16741 tggacgctgc tggccgccct cgccgcccgg acgtcccgga tccgcctcgg cgtcctcgtc
    16801 acgagcaaca cgttccgtca tccggccgtg ctcctcaagg cggccgtcac ggtcgatcac
    16861 atcagcggcg gccggctgat cctgggtctc ggcacgggct ggcacgccga cgagcaccgc
    16921 aggtacggcc tggcgctgcc ggagcccgcg gaacgggtcg accggttcgc ggagtcggtc
    16981 gagctgatca gcctgctcat gtcccaggag cggacgacgt ttcgcggcga ccactaccag
    17041 ctcgacgacg ctccgctcga accgcgaccc gtccagaagc cccggatccc gatcctcatt
    17101 gccgctcatc ggccgcggat gctccgcctc gccgcccgtt tcgcggacca gtgggacacg
    17161 ttcgcggcga tgcccgagac cgcgaccgac ggtgtcgaga cggcgctcgc cgaccggatc
    17221 cgctacctcg atgaggcctg ccgctcgatc ggtcgcgatc ccgccgagat ccgccgatcg
    17281 acgtggacga ccgcggcggc cctccgctcc gaggacgctt acgtcgactt cgtcacccgc
    17341 catcggcagc tcggcttcac cgatttctcg acggtcatgc cggcgaccgg cgacctggcc
    17401 gtgctgcggc gcgtcgccga aggggtgatt ccggagatgc gggccgggcg actctgaggg
    17461 ctgcggtgcg cgctcagccg gctcggccgg tggcggcgac gacggcacgg gtgagccggt
    17521 ccgttcgcag ggcgtcggca taggtcgaca ggacgcgggt cggatcgccc gcctcgacag
    17581 cgtcgaggaa gatcgcggcc tgggcttcgt aggggtcgcg gccgggtggc acggtcgcac
    17641 gggaccggcc gtcgtcgaag acgacctccc agctgccggg cgcgtcaccg acgcggcgga
    17701 tcgtgatgag caggtccgct gccgcgagct cgacctcgac gaccgcggag gccagtcgcc
    17761 gggtgttggc aaagctgccg acggcgccgc tctcgaagcg cagcaccgcc gccgtggcgt
    17821 cgacgacgtc cgcgccggcc gggacgacgg gctcttccct ggtcgaggtg gcgccgatca
    17881 ccgttgcctc gccgacgagg gaccgggcga gatcgtagaa gtgggttgcc tgctcgacga
    17941 cctggccgcc gccgaccttc tcgcgcagcc accaggccgg ggccggcgtg cccgacagcc
    18001 agcgggcggt cacgagatgg acgggctgac cggtgaggcg ctcgcggacc tcgacgagcg
    18061 cgtcgagagc acggaggtgg tatccgacgg ccgcgacgat gccccgctcg gcgacgcctg
    18121 cggcgatccg ggccggaccg gcggcgtcgg tggcggcgag cggcttctcg gtcaggaacg
    18181 ggatgccccg ctcgacgagc cggtccagca ccgcgatcgc ggccgacggc gggacggcga
    18241 cccagacgac gtccgggcgc tgctcatcga gcatccgccc gacgtcgacg taggacggtg
    18301 cgccacgcgg ctctgccatc gcccgggcgc tctcctcacg cgccgacgcg atgccgacga
    18361 gccgggtccg gccgagccgg tcgagcgccg cgagatgggc cggcgtgatc cagcccgcgc
    18421 cgatcattgc cagccggagc tcgccgtccg tggtcgatct cgatcgggcg ctcgccattc
    18481 ggcctcctcg gcgggtctcg accggcgcca ggttgcgccg ccaccacgcc agacgcggat
    18541 cgtgccacag cccggacgcg ctcgggtcgg gctgtggcgc ggccgatctt ccacgccgga
    18601 ctggcgtgcg gtcggggcag gacgctgacc cacccggcgc tgatgtggct acggcgccgc
    18661 cgacgccccc gcggcggacg atgagccggg gctcgcgacc ggccaggtcg gatcggcgcc
    18721 cggtggcccg aacacgacct tcgccatctc ccggatcctg gccgggaccg ggacgaagat
    18781 cgagccgtac tggctcgagg cgccccggat ctgcggcgag ccgaggacca gcttcatcgt
    18841 cgatgacccg ccgatctgct cggccagggc tgcgagttcc gggaggcggt cgggcggcag
    18901 gtccgtccgg accgtggcgc cgaccgcatg gagcaacttc ggcaggctga tcaggatccc
    18961 cgccccgacg gcgcggttgc ggagcgcgac caggacctcc tgctggcggg cggcgcgggt
    19021 caggtcgctc tcgccggccg acttgcggat ccgggcatag gcgagggcgt ccgttccgtc
    19081 gaaatggtga ggccccggct ggacggacca gcctcgcttg ccgtcgaggc ccggatagcg
    19141 cgggtcgtcg agtgccttcc tgacgacgat gtcgacgccg ccgacggcat cgatcatcgc
    19201 cgcgaacccg ccaagatcga ccttggcgta gtagtggatc gggatcccga acagtgcccc
    19261 gatggcgtcc tcgagggcgc ggatcccacc acgttcaaca tccttcggat ggcgatcggc
    19321 ccagctcatc agcgagttga gcttcggccc gaagatgctg ccgttgccga gtgggacgct
    19381 ggcgaggtcg cgtgggatcg agaccatcga cacggtccgt ccgacagggt ccagcgagac
    19441 cacgatcatc gagtccgtca gcgcctccgt ccggccgggc gccgagtcga ccccgatcag
    19501 caggacgttg aggcgctcgc cctcggcggg cggtggcgcg ctcgaaatcg aacggacggc
    19561 cgacccggag aagatctggc cgaacatcgc ctgggccgcg gtgccgtagc tccaggcgag
    19621 gaggtgaggc acggcggtgg cgacgagcag gaccgcgagg cccatcgcgc cgagccgccc
    19681 ggggcgcaac ggatagcgtc gatcgaggaa ggcgtgcagg acggcgacgg cccgccagcc
    19741 gaggatgacg acgttcacga cgaggaggac gccgagcacg gacggcgcga tgacccgggc
    19801 gatcagcatc gtcggccggt cgctctgggc ggtcagccag gccatcccga tcacggccag
    19861 cgtcggaagg ccgaacagca acgccgaccg gagccggccg ttgattgcct gcccgaaccc
    19921 cggcagcagc gacgaggcga ttgcggcgag gaggcggcgc cggtcggtcg gacggctgtc
    19981 cggtcgcgcg ccctgggccg gctgggcgcc gtgggccctg gatgatggcg aaccggtggg
    20041 atccccttgg tcgacctcga tcgatcggcg ctgcgaccgc atcgggcgag cgtggtcggc
    20101 ggcggtcgga cggcgggccc ggagcggcgg ggcagacgga tcgcgcacgg aaggctcggc
    20161 catcgcgggg agtgtagctg caaccccggc acgttgccgc gctcggcggg gcgtgaaggc
    20221 gcttcacgag cgacccgcgg cggacatggt gacgccgggc gatcgatcag cctgccagtg
    20281 atcgcccggc gtcgtgtctg tgacgcgcga cggtcgccga ctgttccgcg acgaatcacc
    20341 aatcgcttcg ctcgacctcg agcccacgca tgtcgtcctc gggaaaccgc cggatgtcag
    20401 ggcaaccgga agacgtggcg gacgcgccgt caaggggcgc ggaacgacgg cgccgttccg
    20461 ataccgctca tagttcgact gagtcggtcg atgaactcct cgtacgacgc cggcgggttg
    20521 ccgtcagaga gcgccgcacc gatccgcgac ttcatctccg agctgaaggc ggcggccgcc
    20581 tcgtcgagga acgccggcat cgtgcagttc tgcaggatgc cgagacggat catccggtcg
    20641 gtgagcgccg tcgtgatgac gatgccgcgg tgctggatcc gtatcgatcg gcctttcccg
    20701 gccacgccgt cagggccgtt gatgatgtcg agcatggccg cgtcgatcgc ggtcccgaac
    20761 tcggccgcga gcgtgtcgca tgtcgtcgcg acaaaggacg ggatcgggtc gcagccgccg
    20821 atgtcggtcg acacgccgtt gcagaacgat tccgtcggtc tggctgacgt ggatgccggg
    20881 ccgcagcccc cgaggatgac ggcgatgacg acgatgacga ctgtggaccg cagggaggtc
    20941 cgccggcgaa ccgtcatgtt gatcacctcc tcgggggcgt gcgcggatca gccggctagc
    21001 aggtgtggaa cgtcctgccg ccggacgtcg tgaccaggac cggatccatc acgtcggact
    21061 tggccgtcac cgtctccaac gtgaccgagc ctcgtgttcc ggacttgtcc gtgaggttca
    21121 gctccgcttc ggcgatgttc ttgtacgtga acgaaccttt tgcgttcatg tagaactcga
    21181 gctcacctga ataggtgacc gtctttgtcc cggtcgccgc aaagccgacc cggtaccaga
    21241 cttcccaata gttgtcatcg gcgccgatgc agtcgccaag ggcggtgccg ctggacggga
    21301 gcagcttcca gggttggctg ttgaccgaga agcaccagaa ctccgggcac ttgtagcgga
    21361 actgggtctc gatccagcag gtgctgatgc cggccgggca gtcgtgcggg tagctgatac
    21421 gggtggtcgg ccggccgtcg gcggtgacgg ccagctcggt gatgctgttg cggttgccga
    21481 ggtcgccgat ctcgccgtcg acggcaagca cgccggtcgg cagaatcagc aaggcgaaca
    21541 acacgaggat cggagcggcc cgcaagacgg gcgaatggtt tcgcaaagtc gtggctccca
    21601 ggcgggaggc gttcgatggc cggttgcgca actggcgcga accatcgcaa agcgctctta
    21661 tccagggctt gatcgcagca acctgcgact tatcaggcgc attggatctt gtctcggatg
    21721 agaggtcgaa tcaggcggtc ttcgtccctg cagcgtcgag ggccaaggcc cgctggaccg
    21781 cgactgcccg aaggtcgggc tcaaccgcga ccggccgccc gcgatccggg ccttggttgc
    21841 cacaccgttg gtgatcgccg tccagtctcc ttcggggcga acgccatcgc cctcttgctt
    21901 gccgccgcac gagcgaacat gtgcacatgc gtgccttcat gacccgcctc gatccccgtc
    21961 gtgctccggc cgtcgtgttg ttgctcgcgc cccccgtcct gctggtgctg atcgtcgggc
    22021 tgacaccgga gccgaccagg ctcatcttcg cgggcgacct ttctgtctac cgcaggtacg
    22081 gcctccaggt gctgggggga tcgatcccgt acctcgactt ccatgtggag tacccgccgc
    22141 tcgctctcgt gccgatgacc ctgccccttc tggcgctgcc gcccggcggg atcgacgacc
    22201 tcggctacat ctggcggttc acccttgccc agggggcgct cgcggccgcc gtcggctggc
    22261 tgctcttcga ggccaccggc aggtcg
//
LOCUS       DATAIW010000457         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_135525, whole genome shotgun sequence.
ACCESSION   DATAIW010000457 DATAIW010000000
VERSION     DATAIW010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135525"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1348
                     /locus_tag="VHM48_13715"
     CDS             77..1348
                     /locus_tag="VHM48_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756520.1"
                     /translation="MSSAPVAEPVVALPARSARPNPDVTLWLLSIAHAVNHAQAVLLP
                     LIYLKIITEFGVTESQVAYLAAIGSLASGVVQLSYAKLTRVVSRRILLTTGGVLFGAG
                     FAAQGLMSSFLPFGLVNVASRIGGSPQHPVGNGLLAEQFPESRRGFAISAHISGGNVG
                     TVVVALVGAGLIATIGWRWVVVVFGVPAVLIALAILLFVRESGVDRRAASEHGSVREA
                     FRTVLRDPSHRWIYLASVLGGGGRGLGVVNLFALLYLTSVIGLPEATTGLMYGVLIVL
                     SVPMPLIAGWLSDRVGRRPVIVGVYVGGAIAFVVFLLAGSAMAGLWLGILLMGLFSFA
                     ESPQLQALLADIAPPTVRDASFALYFTLAFGVGSLWTALYGGIIDVLGDQAGLPIVFW
                     LMAFSFVLAALAVIPIREPRRDRAPAPADLD"
     gene            1424..1500
                     /locus_tag="VHM48_13720"
     tRNA            1424..1500
                     /locus_tag="VHM48_13720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1458..1460,aa:Pro,seq:cgg)
     gene            1572..2783
                     /locus_tag="VHM48_13725"
     CDS             1572..2783
                     /locus_tag="VHM48_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756521.1"
                     /translation="MPTRRTSPRRTATRPGGLYERTPEPPFDDADDAPDAGFLQIAAP
                     WLALLAIVLAAGAVGYVVFGRSSDGDLTACRSAAWGSIPDTKLLPTGWSIGSTDLSAN
                     GMTISLLGATAPDATDQPVVVVSVTCYGGAAETALDANRDAAAAARATVAPRTAAGDA
                     YDVDNPATGGTTTFFRVGGLVAQVADSGTVAQADREAITAAFANAMGDRNAAGIGAAR
                     PTDGATASEEPLGSDDPGAVDPSSSPFAADLEALLPKSIMDNTSTASPPPAIPLTTQS
                     ASATDVFGGDPNARALAARIRALGATLDQLQIAQAFDDTGAFDISIIAFRLPKADLAK
                     LRAAVIDTWLSARAEGVKTTTITLGGKSVTKVDYGDGSTIEYVYAKADYVIVIDTSDL
                     DVATQAAAQLK"
     gene            complement(2833..>3160)
                     /locus_tag="VHM48_13730"
     CDS             complement(2833..>3160)
                     /locus_tag="VHM48_13730"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX2756522.1"
                     /translation="SPPELVERFAAVMAGYPDAVQKKMFGYPAAFVGGNMATGLFADR
                     WVVRLPDEQIEAAKVAGAGSFEPMPGKPMKSFVVIPARDVADDAALRGWVERGLAHAG
                     AMPAKK"
BASE COUNT          419 a         1178 c         1063 g          500 t
ORIGIN      
        1 gacgaagcgc gcctgctgtc gaccggcctg cgagtcgtgc caaccgaggc gctcccgatc
       61 cgtcagcatt cggtcgatgt ccagcgcacc cgttgccgaa cccgtcgtcg cccttccggc
      121 ccgctcggcc cgcccgaacc ccgacgtcac cctctggctg ctgtcgatcg cccacgcggt
      181 caaccacgcc caggccgtgc tcctgccgtt gatctacctc aagatcatca ccgagttcgg
      241 ggtgaccgag tcccaggtcg cctacctggc cgcgatcggc tcgctcgcga gcggcgtcgt
      301 ccagctgagc tacgccaagc tgacccgggt cgtctcgcgg cggatcctcc tgacgaccgg
      361 cggcgtgctg ttcggggccg ggttcgcggc gcagggcctg atgtcgagct tcctgccgtt
      421 cggcctggtg aacgtcgcgt cgcggatcgg cggctcgccg cagcatccgg tcggcaacgg
      481 actgctcgcc gagcagttcc ccgaatcccg ccgcggattc gccatcagcg cccacatcag
      541 cggcggcaac gtcgggacgg tcgtggtcgc cctcgtcgga gccggcctga tcgcgacgat
      601 cggctggcgc tgggtcgtgg tcgtgttcgg cgtgccggcg gtcctgatcg ccctcgcgat
      661 cctgctcttc gtccgggagt cgggtgtcga ccgccgggcc gcgtccgagc acggatcggt
      721 ccgcgaggcg ttccggaccg tgctccgcga cccgtcgcat cgctggatct atctcgcgtc
      781 cgtcctcggc ggcggcggcc gcgggctcgg cgtggtcaac ctgttcgcgc tcctctacct
      841 gacgagcgtc atcgggctgc cggaagcgac gaccggcctg atgtacggcg tgctcatcgt
      901 cctctcggtg ccgatgccgc tgatcgccgg ctggctgtcc gatcgggtcg gccgacggcc
      961 ggtcatcgtc ggggtgtacg tcggcggcgc catcgccttc gtcgtgttcc tgctcgcggg
     1021 ctcggccatg gccgggctgt ggctcgggat cctgctgatg ggcctcttca gcttcgccga
     1081 gtcgccccag ctccaggccc tcctcgccga catcgcgccg ccgacggtcc gcgacgcctc
     1141 gttcgcgctg tacttcacgc tcgcgttcgg cgtcgggtcg ctgtggaccg cgctctacgg
     1201 cgggatcatc gacgtgctcg gcgatcaggc cgggctgccg atcgtgttct ggctgatggc
     1261 gttctcgttc gtgctcgccg cgctggccgt catcccgatc cgggagccgc gccgggaccg
     1321 cgcccctgcg ccggccgatc tggactgacg ccacgatcga tcgggcgtcg tggtggatcg
     1381 cctctgctat gatcggcagg ccctgtgaca acgggcatcc ggtcggggcg tggcgcagct
     1441 tggtagcgcg tatcgttcgg gacgataagg tcgtgggttc aaatcccgcc gccccgacca
     1501 acagacctgc cggtccgccg gcgcgtccaa tctcagacgg ctctcagcgg ccgtccggac
     1561 cgtgaggcca gatgcccact cgacggacca gcccgcgtcg cacggcgaca cgacccggcg
     1621 gactctacga gcgcaccccg gaaccgccgt tcgacgacgc cgacgacgct ccggacgccg
     1681 ggtttctcca gattgccgct ccgtggctgg cgctgctcgc catcgtcctg gccgcaggcg
     1741 cggtcggcta tgtcgtcttc ggccgttcgt cggacgggga cctcaccgcg tgccgttcgg
     1801 ctgcctgggg gtcgatcccc gacacgaagc tgctgccgac tggctggtcc atcggctcga
     1861 ccgacctgag cgccaacggg atgacgatct ctctcctggg tgcgaccgct cccgacgcga
     1921 ccgaccagcc tgtcgtcgtc gtgagcgtga cctgctacgg cggagcggcc gagaccgccc
     1981 tcgacgcgaa ccgcgacgca gcggccgcgg cgcgcgccac cgtggcgccc cggaccgccg
     2041 ccggcgacgc gtacgacgtc gacaaccccg cgacgggcgg aacgacaacg ttcttccggg
     2101 tgggcgggct ggtcgcccag gtcgccgatt ccggcaccgt cgcccaggcg gatcgcgagg
     2161 cgatcaccgc cgcattcgcg aatgcgatgg gcgaccggaa cgcggccggg atcggcgcgg
     2221 cccgcccgac cgacggcgcg actgcgagcg aagagccact cgggtcggac gatccgggcg
     2281 ccgtggaccc gtcctcgtcg ccgttcgcgg ccgatctcga ggccctcctc ccgaagtcga
     2341 tcatggacaa cacctcgacc gcgtcgccac cgccggcgat cccgctcacg acccagagcg
     2401 ccagcgcgac cgacgtcttc ggcggggatc ccaatgcgcg agcgctcgcg gcccggatcc
     2461 gggccctcgg ggcgacgctg gaccagctcc agatcgcaca ggccttcgac gacacgggcg
     2521 ccttcgacat ctcgatcatc gccttccgcc tgccgaaggc cgacctggcc aagctccggg
     2581 cggcggtcat cgacacgtgg ctgtcggcga gagccgaagg cgtgaagacg accacgatca
     2641 cgctcggtgg caagagcgtc accaaggtcg actacggcga cgggagcacg atcgagtacg
     2701 tctacgccaa ggccgactac gtgatcgtca tcgatacgtc cgatctcgac gtcgcgaccc
     2761 aggcggcggc ccagctcaag taggcgtgcg cgcggcgacg cttcggcggc accgccgcag
     2821 cgtaagcctc atctacttct tcgccggcat cgccccggcg tgggcgagcc ctcgctcgac
     2881 ccagccgcgg agcgccgcat catccgcgac gtcccgtgcc gggatcacga cgaacgactt
     2941 cattggcttg ccaggcatcg gctcgaacga cccggcgccg gcaactttgg cggcctcgat
     3001 ctgttcgtcg ggcaggcgaa ccacccagcg gtcggcgaac aggcccgtgg ccatgttgcc
     3061 gccgacgaac gccgccgggt aaccgaacat cttcttctgg accgcatccg ggtacccggc
     3121 catgaccgcc gcgaagcgct cgacgagctc cggcggggac
//
LOCUS       DATAIW010000458         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_226354, whole genome shotgun sequence.
ACCESSION   DATAIW010000458 DATAIW010000000
VERSION     DATAIW010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226354"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VHM48_13735"
     CDS             complement(<1..548)
                     /locus_tag="VHM48_13735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2756523.1"
                     /translation="MSGSYERTNLITSFGFSRRWRLQAVGRLDLRAGDAVHDWMTGMG
                     EGWSPILDRVGGPGRLVAVDLSAGMLGHARRRLERHPDGRVEVIEGDLLELGLPSASA
                     DAVVCLFGVKTLSVPDRARFAAELARVLRPGGRYSLIEVSVPGWPPLRLAYLFYLKRV
                     IPILGRILLGNPENYRMLGVYT"
     gene            complement(653..1855)
                     /locus_tag="VHM48_13740"
     CDS             complement(653..1855)
                     /locus_tag="VHM48_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX2756524.1"
                     /translation="MSVGTAFHPATSPLNRKQQWREWSGYFASSSYADAHDIEYNAIR
                     EAAALIDVSPLFKYVVGGPDARRLVDRVITRDATKLTPGRVFYTPWCDEHGKVIDDGT
                     IHGLDDGSFRWTAADPQLRWLRMNSAGLDVTVDDVTERTAAVALQGPQSRAVLETITG
                     ESFADLGYFRRRTTALKVGRKKVKLDVSRTGYTGDLGYELWIDAEQAVALWDALWAAG
                     QDHAIRPAGMLALDVVRLEAGLILLEVDYTSARHAMNPEQNYSPAEIGLGRLVTFDKA
                     DFVGRLALEREARSGGPARRLVGLELDWYGIEGLFSAQGLPPAISPVVDRSPRPVFVG
                     GRQVGKATSVGWSPILKQAIALASVPPTHEAPGSKLDVEWSVEGRRGRVPAVVVDLPF
                     LDLERKRT"
     gene            complement(1962..>2572)
                     /locus_tag="VHM48_13745"
     CDS             complement(1962..>2572)
                     /locus_tag="VHM48_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756525.1"
                     /translation="PPFTTSAADQPAVGWTYAAVVGGAIVSLIAVVVAGVPMAIAIAR
                     TAVERRRWRNLGLLAVPPISLAVWVALTALLIALGDPPAGDPWRIVIFLVWVGVFIVA
                     ALASTVAVSAAALDAEVDGSLYRRATTPALVTAVAMAVVAAAVVLWGAALAVASPAAF
                     WGSDGILGSSTPLTWLGVVAVMVAATCFAVRAAIRARSDRAT"
BASE COUNT          389 a          902 c          958 g          323 t
ORIGIN      
        1 tcggtgtaga cgccgagcat ccggtagttc tccgggttgc cgaggaggat ccgcccgagg
       61 atcgggatca cccgcttcag gtagaagagg taagcgagcc gcaacggcgg ccagccggga
      121 accgacactt cgatcagcga gtagcgcccg cccggccgca gcacccgcgc gagctctgcg
      181 gcgaaccggg cccggtcagg gacgctcagg gtcttgaccc cgaacaggca gacgaccgcg
      241 tcggccgagg cgctcggcag cccgagctcc aggaggtcgc cctcgatgac ctcgacccgc
      301 ccgtcggggt ggcgctcgag ccggcgccgg gcgtggccca gcatgcccgc cgacagatcg
      361 accgcgacga gccgccccgg cccgccgacg cgatcgagga tggggctcca gccctcaccc
      421 atgcccgtca tccagtcgtg gaccgcatcg cccgctcgga ggtcgagccg cccgaccgcc
      481 tggagccgcc agcgccgcga gaatccgaac gacgtgatca ggttcgtgcg ctcgtacgag
      541 ccggacatct cgtcgaacag ggtccggacg tagccgggat cgtagacgtc gacgaccgcc
      601 gggtggccgg cgccgggcgg gcggatcggc ggcaggcccg ccggcggcgt gctcaggtcc
      661 gcttgcgctc cagatccagg aatgggaggt cgacgacgac cgccggaaca cggccgcgac
      721 ggccctcgac cgaccactcg acgtcgagct tcgagcccgg cgcctcgtgg gtcggcggca
      781 ccgacgcgag ggcgatcgcc tgcttgagga tcgggctcca gccgaccgac gtcgccttgc
      841 cgacctggcg gccgccgacg aacaccggcc gaggcgagcg gtcgaccacc ggcgagatcg
      901 ccggcggcag gccctgcgcg ctgaacaggc cctcgatccc gtaccagtcg agctcgagcc
      961 cgacgagccg gcgggccggg ccgcccgatc gggcctcccg ctcgagcgcg agccgcccga
     1021 cgaagtcggc cttgtcgaac gtcacgagcc ggccgaggcc gatctcggcg gggctgtagt
     1081 tctgctccgg gttcatcgcg tggcgggcgg aggtgtagtc gacctcgagc aggatcagcc
     1141 cggcctcgag ccggacgacg tcgagggcga gcatcccggc cggtcggatg gcgtggtcct
     1201 ggcccgcggc ccagagagcg tcccacagcg cgacggcctg ctcggcgtcg atccacagct
     1261 cgtagccgag gtcgccggtg tagccggtcc gcgagacgtc gagcttgacc ttcttccggc
     1321 cgaccttgag cgccgtcgtc cggcggcgga agtagccgag gtcggcgaag gactcgcccg
     1381 tgatggtctc gaggacggcc cgcgactgcg gtccctggag ggcgacggcg gcggtccgct
     1441 cggtgacgtc gtcgaccgtg acatccagcc cggcgctgtt catccggagc cagcggagct
     1501 gtggatccgc cgcggtccag cggaaggatc cgtcgtcgag gccatggatg gtgccgtcgt
     1561 cgatgacctt gccgtgctcg tcgcaccacg gcgtgtagaa gacgcggccg ggtgtcagct
     1621 tcgtcgcgtc gcgggtgatg acccgatcga ccaggcgacg ggcgtccggt ccgccgacga
     1681 cgtacttgaa cagcgggctg acgtcgatga gggcggccgc ttcacggatc gcgttgtact
     1741 cgatgtcgtg ggcgtccgca tagctcgagg aggcgaagta gcccgaccac tcccgccatt
     1801 gctgtttgcg gttgagcggc gatgtggcgg gatggaacgc ggttccgacg ctcacgggcg
     1861 ggtggacctc cggcgactgg cgaggttcca gtatgaatga ttgttatgca ctcggagcgg
     1921 tcgtcgcgcc ccgtgccgca cggcaccggc gatcgcttgc gtcaggtcgc ccggtcgctc
     1981 cgggcgcgga ttgcagcccg gacggcgaag caggtcgcgg cgaccatgac cgcgacgacg
     2041 ccgagccagg tcagcggtgt gctcgatccg aggatgccgt cggaacccca gaacgccgcc
     2101 ggggacgcca cggcgagggc ggcgccccac aggacgaccg cggcggcgac cacggccatc
     2161 gcgacggcgg tgacgagagc cggggtcgtg gcccgacgat agagcgaccc gtcgacctcg
     2221 gcgtcgaggg cggccgcgct gaccgcgacc gtgctggcga gcgccgccac gatgaagacg
     2281 ccgacccaga cgaggaagat cacgatccgc cagggatccc cggccggcgg gtcgccgagc
     2341 gcgatgagca gcgccgtcaa cgcgacccag accgcgagcg agatcggtgg cacggccagc
     2401 aggcccaggt tgcgccagcg ccggcgttcg acggccgtcc gcgcgatcgc gatcgccatc
     2461 gggacgccgg cgacgacgac ggcgatcagg ctgacgatcg cgcccccgac gacggccgcg
     2521 taggtccagc cgacagccgg ctggtcggcg gcgctggtgg tgaacggcgg cc
//
LOCUS       DATAIW010000459         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_135561, whole genome shotgun sequence.
ACCESSION   DATAIW010000459 DATAIW010000000
VERSION     DATAIW010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135561"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VHM48_13750"
     CDS             <1..871
                     /locus_tag="VHM48_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2756526.1"
                     /translation="AGTEVGDAIVDNPDVAVISFTGSSATGRHIAVHAGRQLKRLSLE
                     LGGKNAIVVMADADLDLATDGIVWSAFGTTGQRCTAASRVVVERAVVEPLLERLEARS
                     RTLRLGSGLDDAVDVGPLINVAAVDKVGRYVGIGRGEGELVLGGEAARDGALVNGHFF
                     APTIFRDVAPMDRIAQEEIFGPVLSVIAVDDYAAATTAVNQTRYGLSSSIFTRDANTA
                     FRAMRDFETGIVYVNAGTTGAETHLPFGGWKETGNGHREAGHAALDTFTEWKSIYVDF
                     SGRLQRAQIDNQE"
     gene            947..1456
                     /locus_tag="VHM48_13755"
     CDS             947..1456
                     /locus_tag="VHM48_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756527.1"
                     /translation="MGERSPAPTIEIRPVTHDDLERLIDIYLDTAIHHAAIDPAWFQV
                     PARDDVAVRLRRRIDGRGEDGEYVAAMLDRQMVGSATISVEDAPHPGSMMRPIRTAEF
                     GVSVVDGHRGLGIGRALIGHLEGWAADHGVERIILNVAEANTGAIRLYHELGYVDYDR
                     AMRKDIGPA"
     gene            1453..1791
                     /locus_tag="VHM48_13760"
     CDS             1453..1791
                     /locus_tag="VHM48_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX2756528.1"
                     /translation="MTTFDDVRRIALLLPEAEERLTWETDITFRVRDKIFAIGGEGAT
                     AVSIKAGLETQAELVEMDPATFAKSAYVGRFGWVTVQLDRVDPALLTSLLREAWRRTA
                     PKRLAATLED"
     gene            1793..2575
                     /locus_tag="VHM48_13765"
     CDS             1793..2575
                     /locus_tag="VHM48_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2756529.1"
                     /translation="MTAPDQTLPEHVKRNRAAWDEWAPEYVANGEVSWRLEPGEEKWG
                     VWDIPERDVRLLPDDLAGKDTIELGCGTAYVSAWLARRGARPVGIDNSEQQLATARRL
                     QTEHGLSFPLVHGNAEAVPYPDASFDLAISEYGASIWADPHRWIPEAARLLRPGGRLI
                     FLINATLLMLCMPDEERPATNELVRPLRGLHRLEWSSDQSVNFALGHGDWIRVLRGSG
                     FAIENLVELWPAEGATTTFPYATLEWARQWPTEEVWIARREG"
     gene            2642..4942
                     /locus_tag="VHM48_13770"
     CDS             2642..4942
                     /locus_tag="VHM48_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2756530.1"
                     /translation="MLLPDPSPRSTRRPPWTARVAGWSARHRWPVFALWFVVTIGIFI
                     ASLAAGGTRAVEAVSNDQRAKYEAGEAYVVYAEANASVGQQEPASQQFLMLVSSPDRT
                     VDDPAFATDVAAITARLAALQATVDGVTGPVMEQLVDPIGVPAAAGLVSADRTTVRLV
                     GRVPGDGAILASRLKPLPKAIDDIKAAYPAYRIHGLSNTLANNEISALITDGLDSSLR
                     LTIPLTFAILLVAFGAVIAAVIPLVLAITALLAAFGILGLYSQAISAVSPYASQLVVL
                     IGLAVAVDYSLFMVTRFRTERRHGRSKLAAIDVASNTAGRAVFFSGLAVMISIGGLFL
                     LDDPLFRSMAVGTISVVFVAVVGSLTFLPATLAILGDGVNRLRIPLVGRDRPEGHGLW
                     ASLVRAVMRRPVIAAVAAAGLLIVVATPVTRLQMGVLNDLTAFPDSLDSVQAVNLLNE
                     KWPTGSDLDLSVVVTRADEAPTKAAIEKMTSAALAIPGLSGPPTTTMSSDGHVAFINY
                     IMAGSSNDTHNRDIVREVRSQVVPTAFAGLAGVRALVTGDAAYTVDIVDFYARGMPLV
                     MVFVLALSFLLLLVAFRSIVIPIKAIILNLLSTGAAFGLQVLVFQDGWLHDQLGFKPG
                     PIESFIPVFIFTILFGLSMDYHVFILTRIKEARDHGLSSNEATARGIAITSGTVTSAA
                     AIMVVVFSVFLTLELTIIKQLGFGLAVAVFIDATIVRSILLPASMRLLGDWNWWLPSW
                     LGWLPRVTIEGDIDEDETGTAPVPAT"
     gene            5027..5254
                     /locus_tag="VHM48_13775"
     CDS             5027..5254
                     /locus_tag="VHM48_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756531.1"
                     /translation="MSLDVVGKLARVTADITPGHLGEVLIEIRQGTERFLARSADKDA
                     TIPKHTQVLVVGSLGGRTVEVSPTDSMRLPR"
     gene            5251..6849
                     /locus_tag="VHM48_13780"
     CDS             5251..6849
                     /locus_tag="VHM48_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2756532.1"
                     /translation="MTSEVGLAIVAIVVLLVLFALFLLFRASWRVAEPDEALIISGLR
                     ASGRQEGTGESMGFNIVTGRGALILPGVTKVRRLSLEAHESEISVPCVSQQKIRVDLR
                     GIVVYKVGDDFRSIANAARRFLDRPAAELETKVQNVFVGHLRAIAGSMTVEDMISDQD
                     KFTQQVRDRCTQEMESFGLVIDSFQIQAITSPSAYIENLAVPHQAEVEQNARIARANA
                     EREAVGQEQAARALMAESTRNADIKSAGYQADIDQATKQAEQQGPLAEAKARQAVVEQ
                     ETRVAELQAQQTEQQLQVDVRKPADAEAYRQTTLAAAARDVRIRQAEAAAQEVRLAAE
                     AAAAQTRIAAQAQAEAVKVQAEAQAGATRLNGEADGDAIRAKGLAEADAVRARMEAEA
                     AGIERRAAALSHNQEAVIAQQIATNLPEIVRAAASSFDHVGNFTVLNGAGGITEVLAE
                     LIQQGGALANVARQTMAPMIGAQSGNGSAPSGDHAIGPDNRPATQATPTASRARRGSA
                     RAANPAAAPGVSAAAAGSDEADER"
     gene            6932..7990
                     /locus_tag="VHM48_13785"
     CDS             6932..7990
                     /locus_tag="VHM48_13785"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX2756533.1"
                     /translation="MAAGFVDAIVAADAHGDVYRRLLADVGEIGEREIVATSELSHRL
                     VDRPMRAIIGTLDGESPIARSLAELREAVEELDPGRLGRSGGRQRRLLGVIPLGDPVD
                     DALERYTKARVRIQRILGSLAVARTGLQAEIAAIGQEQRALATEIETLRQYADLARAL
                     DERLDERLATLASSEPQRAEALRTDVQFAIRQRRRDLLIQLAVATQGDAALRVVQDNN
                     QQVVRTIRMATTTTAAALRTSVAVAQAMADQRRILAQLATVREVDRTVPRDAEAPRVP
                     SSPDVARRAAVGVDLAGLKRAWDTVFATLDQFDSYSVTAIDAMREAVEELAEEAERSR
                     AALEADRALGGSSLLPPP"
BASE COUNT         1201 a         2854 c         2775 g         1160 t
ORIGIN      
        1 cgcgggcacg gaggtcggcg acgcgatcgt cgacaacccc gacgtcgcgg tgatctcgtt
       61 caccggctcg tcggcgacgg gccgccacat cgccgtccac gccggccgcc agctgaagcg
      121 gctcagcctc gagctcggcg gcaagaacgc gatcgtcgtc atggccgacg cggacctcga
      181 tctcgccacg gacgggatcg tctggtcggc gttcgggacg accggccagc gctgcacggc
      241 ggccagccgg gtcgtcgtcg agcgggcggt cgtcgagcca ttgctcgagc ggctggaggc
      301 ccggtcgcgg accctccgtc tcgggtccgg gctggacgac gcggtcgacg tcgggccgct
      361 gatcaacgtc gccgcggtcg acaaggtcgg gcggtatgtc ggcatcgggc ggggtgaggg
      421 cgagctcgtc ctcggcggcg aggcggcacg ggacggagcg ctggtcaacg gccatttctt
      481 cgccccgacg atcttccgcg atgtcgcccc gatggaccgg atcgcccagg aggagatctt
      541 cggccccgtc ctgtcggtga tcgccgtcga cgactacgcg gccgcgacga ccgcggtcaa
      601 ccagacccgc tacgggctga gctcctcgat cttcacccgg gacgcgaata ccgccttccg
      661 ggcgatgcgc gacttcgaga ccgggatcgt gtacgtcaac gccggcacga ccggagccga
      721 gacgcatctc ccgttcggcg gctggaagga gaccggcaac ggccaccgcg aggccgggca
      781 tgccgccctc gacacgttca ccgagtggaa gtcgatctac gtcgacttca gcgggaggct
      841 acaacgtgcc cagatcgaca atcaggaata ggccatgggt cgcaccgagg tccgaggggg
      901 agctccgcga ccccatcggc ccgaggttcg acaaccagga gtagcagtgg gggagcgctc
      961 gcccgcgccg accatcgaga tccggccggt cacccacgac gatctcgagc gcctgatcga
     1021 catctacctc gacaccgcga tccaccacgc cgccatcgat cccgcctggt tccaggtgcc
     1081 cgcccgcgac gatgtcgcgg tccgcctccg gcgccggatc gacggccgcg gggaggacgg
     1141 cgagtacgtc gcggcgatgc tcgaccggca gatggtcggc tcggcgacga tctccgtcga
     1201 ggacgcgcct cacccgggct cgatgatgcg cccgatccgc accgccgaat tcggcgtgtc
     1261 ggtcgtcgac ggccatcgtg ggctcggcat cggccgggcg ctgatcggcc atctcgaagg
     1321 ctgggcggcc gaccacggcg tcgagcggat catcctcaac gtcgccgagg cgaacacggg
     1381 cgccatccgc ctctatcacg agctcggcta tgtcgattac gaccgggcga tgcgcaagga
     1441 catcggcccc gcgtgacgac cttcgacgat gtccggcgga tcgcgctcct cctgcccgag
     1501 gccgaggagc gcctgacctg ggagaccgac atcacgttcc gggtccgcga caagatcttc
     1561 gcaatcggcg gtgagggagc gaccgcggtc tcgatcaagg ccggcctcga gacccaggcc
     1621 gagctggtcg agatggaccc ggcgaccttc gcgaagtccg cctacgtcgg gcggttcggc
     1681 tgggtcaccg ttcagcttga ccgggtcgac cccgcgctcc tcacgagcct gctccgcgag
     1741 gcatggcggc ggaccgcgcc gaagcgcctc gccgccaccc tggaggactg agatgaccgc
     1801 ccccgaccag accctccccg agcacgtcaa gcgcaaccgg gccgcctggg acgaatgggc
     1861 gcccgaatac gtcgcgaacg gcgaggtgag ctggcgcctc gagcccggcg aggagaagtg
     1921 gggcgtctgg gacatcccgg agcgggacgt tcgcctcctg cccgacgatc tcgcgggcaa
     1981 ggacacgatc gagctcggct gcggcaccgc ctacgtctcc gcgtggctgg cgcggcgcgg
     2041 ggcccggccg gtcgggatcg acaactccga gcaacaactc gccaccgccc gtcgcctcca
     2101 gaccgagcat ggcctctcgt tcccgctggt ccacggcaac gccgaggccg ttccatatcc
     2161 cgacgccagc ttcgacctgg cgatctccga gtacggcgcg tcgatctggg ccgaccccca
     2221 tcgctggatc ccggaggcgg ctcgtctcct ccggccgggc ggccggctga tcttcctcat
     2281 caacgccacg ctcctgatgc tgtgcatgcc cgacgaggag cggccggcga ccaatgagct
     2341 cgtccggccg ctgcgcggcc tccatcggct cgagtggtcg agcgaccagt cggtcaactt
     2401 cgccctcggc catggcgact ggatccgcgt cctccggggg agcgggttcg cgatcgagaa
     2461 cctcgtcgaa ctctggcccg ccgagggcgc gacgacgacg tttccgtacg ccaccctcga
     2521 gtgggcgcgg cagtggccga ccgaggaggt ctggatcgct cgccgggagg gctgagcgtc
     2581 gacacggaat ccggcggagg gcgggccgcc gccagccatg tcgggtaccc tgaccgccgc
     2641 gatgcttctg cccgacccgt caccgcgcag caccaggcga ccgccgtgga cggcgcgcgt
     2701 ggcgggttgg agcgcccggc atcggtggcc ggtcttcgcc ctctggttcg tggtcacgat
     2761 cgggatcttc attgccagcc tggcggccgg tgggacgcgc gcggtcgagg cggtctcgaa
     2821 cgaccagcga gccaagtacg aggcgggcga ggcctacgtc gtctacgcgg aggccaacgc
     2881 gtcggtcggt cagcaggagc cggcctcgca gcagttcctg atgctcgtct cgagtcccga
     2941 tcggaccgtg gacgatccgg cgttcgccac cgacgtcgcg gcgatcaccg cgcgcctcgc
     3001 cgccctccag gcgacggtcg atggggtgac cgggccggtg atggagcagc tcgtcgaccc
     3061 gatcggcgta ccggccgcgg ccggtctcgt gtcggccgat cgcacgaccg tccggctcgt
     3121 cggccgggtg ccgggcgacg gcgcgattct cgccagccgg ctcaagccgt tgccgaaggc
     3181 gatcgacgac atcaaggctg cctatccggc gtaccggatc cacggtctca gcaacaccct
     3241 ggcgaacaac gagatcagcg cgctcatcac cgacggactg gattcgtcgc tgcggctgac
     3301 catcccgctg acgttcgcca tcctgctggt cgccttcggg gccgtcatcg ccgcggtcat
     3361 cccgctggtc ctcgcgatca ccgcgctcct cgccgcgttc gggattctcg gcctctacag
     3421 ccaggcgatc tccgcggtca gcccgtacgc gagccagctc gtcgtcctga tcgggctcgc
     3481 cgtcgcggtc gactactcgc tattcatggt cacccggttc cggacggagc gccgccacgg
     3541 ccgctcgaag ctggccgcga tcgacgtcgc cagcaacacc gccggtcggg ccgtgttctt
     3601 ctcgggtctc gcggtgatga tctcgatcgg cggcctgttc ctgctcgacg acccgctgtt
     3661 ccgatcgatg gccgtcggca cgatctcggt cgtgttcgtc gcggtcgtcg gctcgctgac
     3721 cttcctgccg gcaacgctgg cgatcctcgg cgacggggtg aaccggctgc ggatcccgct
     3781 ggtcggccgc gaccgacccg agggacacgg cctgtgggcg tcgctcgtcc gggcggtcat
     3841 gcgccggccg gtgatcgccg cggtggccgc ggcgggtctc ctgatcgtcg tcgcgacgcc
     3901 ggtgacccga ctccagatgg gcgtcctgaa cgacctgacg gccttccccg actcgctcga
     3961 cagcgtccag gcggtgaacc tgctcaacga gaagtggccc accggctccg atctcgatct
     4021 gtcggtggtg gtgacccgcg ccgacgaggc gccgacgaaa gcggcgatcg agaagatgac
     4081 gtcggccgcg ctcgcgatcc cggggttgag cggcccgccg acgacgacga tgtcctcgga
     4141 cgggcacgtc gcattcatca actacatcat ggccgggagc agcaacgaca cccacaaccg
     4201 cgacatcgtc cgcgaggtcc gctcgcaggt cgtgccgacc gccttcgccg gtctggcggg
     4261 tgttcgcgcc ctcgtcaccg gcgacgccgc gtacaccgtc gacatcgtcg acttctacgc
     4321 ccgcgggatg ccgctggtga tggtcttcgt cctcgccctg tcgttcctgc tcctgctcgt
     4381 cgccttccgc tcgatcgtca tcccgatcaa ggcgatcatc ctcaacctcc tctcgacggg
     4441 ggccgcgttc ggtctgcaag tcctcgtctt ccaggacggc tggctgcacg atcagctcgg
     4501 cttcaagccg ggcccgatcg agtcgttcat cccggtcttc atcttcacga tcctgttcgg
     4561 gctgtcgatg gactaccacg tcttcatcct cacccggatc aaggaagcgc gggaccacgg
     4621 tctctcgtcc aacgaggcga ccgcccgggg catcgcgatc acgtcgggga ccgtcacgag
     4681 cgccgccgcg atcatggtcg tcgtcttctc ggtgttcctt acgctcgagc tgacgatcat
     4741 caagcagctc ggcttcggcc tcgcggtcgc cgtcttcatc gacgcgacga tcgtccggag
     4801 catccttctg ccggcctcga tgcggctcct cggcgactgg aactggtggc tgccgtcgtg
     4861 gctcggctgg ctgccccgcg tcacgatcga gggcgacatc gacgaggacg aaaccggcac
     4921 ggccccggtg ccggcgacct gaccgatccc gggcagggca cccgcgcgtt cggaaggcta
     4981 cgatgacggg gcttcggttc cgaacgatgg ggcaggtgag ggaggcatga gtctcgacgt
     5041 cgtcggcaag ctcgcacggg tcacggcgga catcacgccg ggccacctcg gcgaggtgct
     5101 gatcgaaatc cggcagggca ccgaacgctt cctggcacgc tcggccgaca aggacgcgac
     5161 gatcccgaag cacacccagg tcctcgtcgt cggcagcctc ggcggccgga cggtcgaggt
     5221 atctccgacc gattccatga ggcttccgcg atgacatcag aggtggggct ggcgatcgtc
     5281 gccatcgtcg tgctgctcgt tctctttgcg ctgttcctgt tgttccgcgc cagctggcga
     5341 gtggccgaac cggacgaggc cctgatcatc tccgggctcc gggcgagcgg ccgccaggag
     5401 gggaccggcg agagcatggg cttcaacatc gtgaccggcc gtggggcgct gatcctgccc
     5461 ggcgtgacga aggttcggcg cctctcgctc gaggcccacg agagcgagat cagcgttccg
     5521 tgcgtgagcc agcagaagat ccgggtcgac ctccgcggca tcgtcgtcta caaggtcggc
     5581 gacgacttcc ggtcgatcgc gaacgccgcc cggcggttcc tcgatcggcc ggcggccgag
     5641 ctcgagacga aggtccagaa cgtcttcgtc ggccacctcc gggcgatcgc cgggtcgatg
     5701 accgtcgagg acatgatcag cgaccaggac aagttcaccc agcaggtccg cgaccgctgc
     5761 acccaggaga tggaatcgtt cggcctggtc atcgacagct tccagatcca ggcgatcaca
     5821 agccccagcg cctacatcga gaacctcgcc gtcccgcacc aggctgaggt cgagcagaac
     5881 gcccggatcg cgcgggcaaa cgccgagcgc gaggccgtcg gccaggagca ggcggcgcgt
     5941 gcgttgatgg cggaatcgac ccgcaacgcc gacatcaaga gcgccggcta ccaggccgat
     6001 atcgaccagg cgaccaagca ggccgagcag cagggcccgc tggccgaagc caaggcccgc
     6061 caggcggtcg tcgagcagga gacccgcgtc gccgagctgc aggcccagca gaccgagcag
     6121 cagctccagg tcgatgtccg caagccggcc gatgccgagg cctatcgcca gacgacgctc
     6181 gcggccgcgg ctcgcgacgt ccggatccgg caggccgaag ccgccgccca ggaggttcgc
     6241 ctcgccgcgg aggcagctgc ggcgcagacg cggatcgcgg cccaggccca ggccgaggcg
     6301 gtcaaggtcc aggccgaagc ccaggcgggc gccacgcgcc tcaacggcga ggccgacggc
     6361 gatgcgatcc gggccaaggg tcttgccgaa gctgacgcgg tccgcgctcg catggaagcc
     6421 gaggccgccg ggatcgagcg tcgggccgcg gccctcagcc acaaccagga agcggtcatc
     6481 gcccagcaga tcgcgaccaa tctcccggag atcgtccggg ccgccgcgtc gtcgttcgac
     6541 cacgtcggga acttcaccgt cctcaacggc gccggcggga tcactgaggt cctcgccgag
     6601 ctgatccagc agggcggcgc cctcgccaac gtcgcgcgcc agacgatggc accgatgatc
     6661 ggggcccaga gcggcaacgg atcggcgccg agcggcgatc acgcgattgg acccgacaat
     6721 cgcccggcga cgcaggccac gccgaccgcg agtcgggcgc gtcggggatc ggcccgggcg
     6781 gccaatccag ccgccgcgcc gggcgtctcg gctgcggcgg caggctccga cgaagcggac
     6841 gagcggtagc ctgggcgacc cgatcccgga gaccgcccaa tccggcgggg gtccggcgaa
     6901 cgaaccggtc ctcgccaccc ggatcgacgc gatggcggcc ggttttgtcg atgcgatcgt
     6961 cgccgcagac gcccacggcg atgtctaccg gcggctgctc gccgacgtcg gcgagatcgg
     7021 tgagcgcgag atcgtcgcga cgtccgagct gagccatcga ctggtcgacc ggccgatgcg
     7081 ggcgatcatc ggcacgctcg atggcgagtc gccgatcgcg cggagcctgg ccgagctgcg
     7141 cgaggccgtc gaggagctgg atcccgggcg cctcggtcgg tccggcggac ggcagcgcag
     7201 gctgctcggc gtgatcccgc tcggcgatcc ggtcgacgat gccctcgagc gctacacgaa
     7261 ggcgcgggtc cggatccagc ggatcctcgg ctcgctggcg gtcgctcgga cggggctcca
     7321 ggccgagatc gccgccatcg gccaggagca gcgggcgctc gcgaccgaga tcgagacgct
     7381 ccgccagtat gccgaccttg cgcgggccct cgatgaacgc ctcgatgaac gcctggccac
     7441 cctcgcgtcc agcgagccgc agcgggccga ggcgctgcgg accgatgtcc agttcgcgat
     7501 ccgccagcgt cgccgggacc tcctgatcca gctggcggtc gcgacccagg gcgacgccgc
     7561 gctgcgggtg gtccaggaca acaaccagca ggtggtgcgg acgatccgga tggcgacgac
     7621 gaccacggcc gcggcccttc ggacctccgt ggcggtcgcc caggcgatgg ccgaccagcg
     7681 acggatcctc gcccagctcg cgacggttcg cgaggtcgat cgaaccgttc ctcgagacgc
     7741 cgaggcgccg cgcgtgccgt cgtcgccgga tgtcgcgcgg agggccgccg tcggcgtcga
     7801 cctcgccggg ctgaagcggg cctgggacac cgtgttcgcg accctcgacc agttcgattc
     7861 ctactccgtc acggccatcg acgcgatgcg ggaggcggtc gaggagctgg cggaggaggc
     7921 ggagcgatcg agggccgcgc tggaagcgga tcgggcgctc ggcggatcgt cgttgctgcc
     7981 gccgccctga
//
LOCUS       DATAIW010000460         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_135627, whole genome shotgun sequence.
ACCESSION   DATAIW010000460 DATAIW010000000
VERSION     DATAIW010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135627"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VHM48_13790"
     CDS             complement(<1..1328)
                     /locus_tag="VHM48_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2756534.1"
                     /translation="MPDLVTEVPGPKARAHVAYDETWTSPSLPRAYPIVPVRGDGLVV
                     EDIDGNLFLDFAAGIAVTSTGHSHPQVVGAIREQAGDLIHFSASDFYLPIYAQTCQRI
                     AELTPIAGGRARTYLGNSGAEVVEASIKLARYATRRPYVVAFLGAFHGRTYGAVSLTA
                     SKAKYHAGFGPLLPGVFHAPYGKVADLRWFDEVLFDRLVPANEVAAIIVEPIQGEGGY
                     IVPEDGFLQGLRAICDKHGILLIADEIQSGAGRTGTMWAVEHWGVKPDILLTAKGIAS
                     GMPIGAMVARADLLEAWGVGAHGSTFGGNPIACAAALATIDLLEGGLVDNAAARGAQA
                     IAGLAPLQIQYPSLVRDVRGRGLMLGVEFDTAEHAEEVQWEAFQRGLLVLECGKSSVR
                     MSPALIVTEAEMATALRIFAAAVADVAGEPADHPIVHAAEAAHAMAGPEAA"
     gene            1527..>2799
                     /locus_tag="VHM48_13795"
     CDS             1527..>2799
                     /locus_tag="VHM48_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX2756535.1"
                     /translation="MNSRTFRKSAIGIVVTAGLLAGVAGLVWVLEERVGVPNASSVFL
                     LAVVVAASRFGTRTAIGTAIGAFLVYDLFFIEPVLTLTVADPGEWLNLVLLLVVGIVV
                     GRLAAGQRDRAEAALVREHEAVSLFRVSRALATRSSTADVLPALASILRAEMEMDRIR
                     ITVATVAGLERMAVDTEAVKAVGDSAASDGAGTDAARAAPWTPPASHHALRRTPGDAP
                     AEWVRVHQQTGEARGRGDSAAYRVIIEVGDRRLGSIWAVRPRSEGPPDGAQTLLLSAA
                     ADQIGQALEQDRLGREAREMEIARRSDALKTALLESVSHDLRTPLTSIRAAAGTFLDR
                     EVPLDAAAQEATAQAIDREAEHLNRLVTNLLDLSRIEAGELQAQPEPFELAGLVERAV
                     ERLRPRLAGRTVELDVPDALPPVLVDEVFIDQ"
BASE COUNT          389 a         1026 c         1000 g          384 t
ORIGIN      
        1 gcggcttcgg gccccgccat cgcgtgggca gcttcggcgg cgtgaacgat cggatggtcg
       61 gcgggctccc cggcgacgtc cgccacggcg gcggcgaaga tccgcagcgc ggtcgccatc
      121 tcggcctcgg tcacgatcag ggccggcgac atccggaccg acgacttgcc gcactccagg
      181 acgagcagcc cgcgctggaa ggcctcccac tggacctcct cggcgtgttc cgcggtgtcg
      241 aactcgacgc ccagcatcag gccgcggccg cgcacgtccc ggacgagcga cgggtactgg
      301 atctgcagcg gcgcgaggcc ggcgatcgcc tgcgctccgc gcgccgccgc gttgtcgacc
      361 aggccgccct cgagcaggtc gatcgtggcg agggccgccg cgcaggcgat cgggttgccg
      421 ccgaacgtgg agccgtgggc gccgacgccc cacgcctcga gcagatcggc ccgggcgacc
      481 atcgccccga tcggcatccc gctcgcgatc cccttggcgg tcagcaggat gtccggcttg
      541 acgccccagt gctcgaccgc ccacatcgtg ccggtccgcc cggcccccga ctggatctcg
      601 tcggcgatca gcaggatccc gtgcttgtcg cagatcgccc ggaggccctg gaggaagccg
      661 tcctctggga cgatgtaccc gccctcgccc tggatcggct cgacgatgat cgccgcgacc
      721 tcattcgcgg gcacgagcct gtcgaacagg acctcgtcga accagcgcag gtcggccacc
      781 ttcccgtacg gggcgtggaa cacgcccggc aggagcggcc cgaaccccgc gtgatacttc
      841 gccttcgatg cggtcagcga cacggcaccg tacgtccggc cgtggaacgc accgaggaac
      901 gcgaccacgt acggccggcg cgtcgcgtag cgggcgagct tgatcgaggc ctcgacgacc
      961 tccgcgcccg agttgccgag gtacgtccgc gcccggccgc cggcgatcgg agtgagctcg
     1021 gcgatccgct ggcaggtctg ggcgtagatc gggaggtaga aatcactggc gctgaaatgg
     1081 atcaggtcgc cggcctgctc ccggatcgcg ccgaccacct gcgggtggga atgacccgtc
     1141 gaggtgaccg cgatcccggc cgcgaagtcg aggaacaggt tgccgtcgat gtcctcgacg
     1201 accaggccgt cgccccggac cggcacgatc ggatacgccc gcgggaggct cggcgacgtc
     1261 caggtctcgt cgtacgccac gtgggcgcgg gccttcgggc cgggcacctc ggtgacgaga
     1321 tcgggcacgg gccgatcccc ggcgagacgg ttctggacgg acgtcacacg gcacctcgaa
     1381 cgacgctggg cgcatacgga gtatgcagac tggatattcc tggccgagca tagcagaggg
     1441 tcatgccccg agaccccctg ccgggcagcc ccgggcccgc ctgccgatcg tcccgggacc
     1501 ccctcaattg gcctacgatc cagcgcgtga acagtcgaac gttccggaag tcggcgatcg
     1561 gcatcgtcgt gacggcaggc ctgttggccg gcgtggccgg gctcgtctgg gtcctcgagg
     1621 agcgggtcgg ggtgccgaac gcctcgtccg tcttcctcct cgcggtcgtc gtcgcggcga
     1681 gccggttcgg gacgaggacc gcgatcggca cggcgatcgg cgcattcctc gtctacgacc
     1741 tcttcttcat cgagcccgtc ctgacgctca cggtcgccga ccccggggag tggttgaacc
     1801 tcgtcctcct cctcgtcgtc gggatcgtcg tcggccggct ggcggcgggt cagcgcgacc
     1861 gcgccgaggc cgccctcgtc cgcgagcacg aagccgtctc gctgttccgg gtcagccggg
     1921 cgctggcgac ccggtcgtcg acggccgacg tcctgcccgc gctcgcctcg atcctgcggg
     1981 cggagatgga gatggacagg atccggatca ccgtcgcgac ggtggccggg cttgagcgca
     2041 tggccgtcga cacggaggcg gtgaaggccg tcggcgattc ggccgcgagc gacggcgccg
     2101 gcacggatgc ggcgagggcc gctccgtgga ccccgccggc ctcgcatcac gccctccgtc
     2161 ggacgcccgg cgatgcgccg gccgaatggg tgcgcgtcca ccagcagacc ggcgaggcac
     2221 gcggccgtgg cgacagcgcc gcgtaccgcg tgatcatcga ggtcggcgat cgtcgcctcg
     2281 gctcgatctg ggccgttcga ccccggagcg agggcccgcc ggacggcgcg cagacgctgc
     2341 tcctgtcggc ggcggccgac cagatcggcc aggccctcga gcaggaccgc ctcggccggg
     2401 aggcgcggga gatggagatc gcccggcgga gcgacgccct caagacggcc ctgctcgaat
     2461 cggtatccca cgacctgcgg acgccgctca cctcgatccg cgcggcggcc gggacgttcc
     2521 tcgaccgtga ggtcccgctc gatgccgcgg cccaggaggc cacggcccag gcgatcgacc
     2581 gtgaagctga gcacctgaac cgcctcgtga ccaacctgct cgacctgagc cggatcgagg
     2641 ccggtgagct ccaggcgcaa ccggagccct tcgagctggc cgggctcgtc gagcgggccg
     2701 tcgagcggct ccgcccgcgg ctggccggac ggaccgtcga gctcgacgtg cccgacgcgc
     2761 ttccgcccgt cctcgtcgac gaggtcttca tcgaccagg
//
LOCUS       DATAIW010000461         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_255020, whole genome shotgun sequence.
ACCESSION   DATAIW010000461 DATAIW010000000
VERSION     DATAIW010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255020"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHM48_13800"
     CDS             complement(<1..593)
                     /locus_tag="VHM48_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX2756536.1"
                     /translation="MAKTSGPAAGNGTDPVTNLPDRRADDRTYDAIVIGGGHNGLVNG
                     AYLAKSGLRTLILERRHLVGGAAITEELRPGFWFTTFSYALSLIRPDIIHELELAKHG
                     FMPLLMPSTFAPMENGDYLLLGQDHGENLREIARHSQHDADAYEAFNHDVLRVCRAIK
                     PLLDQVPPDIFSNDPEELVALASLGSRFRSMDKKVLH"
     gene            complement(593..1792)
                     /locus_tag="VHM48_13805"
     CDS             complement(593..1792)
                     /locus_tag="VHM48_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX2756537.1"
                     /translation="MIRTTPFHDRTSAANVSGLWEHWSGYLVAQKYQLSEKFEYFAIR
                     NSAGLFDSSPLYKYRIHGPDAEAYLAGILARDIRTCQVGHGQYTAWLDDRGFVVEDGV
                     VFRTAADEYLLTCAEPNLAYFAGLVGRHRVTIDDVSEDSAVLSVQGPKSREILACLAP
                     AVADLPYFGLTDATIAGTPVRISRTGYTGDLGFELWIPADRAIAVWDAVGDASAGRGV
                     IPFGLVALYMARIEAGLLLLDVDFGSSRYAWTDANRSTLIELGLGWMVRDLAADQRRF
                     IGRAAIERELRDKSSRWKLTGLVLDWQDYDRHYDEAGLIPPKDHTPIQDEYYVYDDGG
                     TQLGYATSLMYSPMLQRHIALARIPLDRTKPGSRVKLELPVNHRYEYFDAAVSRLPLY
                     NPDRRTA"
BASE COUNT          325 a          726 c          802 g          346 t
ORIGIN      
        1 tcgtggagga ccttcttgtc catcgatcgg aagcgcgagc cgagggacgc gagggcgacg
       61 agctcctccg ggtcgttgct gaagatatcg ggcgggacct ggtcgaggag cggcttgatc
      121 gcccggcaga cccgcagcac gtcgtggttg aaggcctcgt aggcatcggc gtcgtgctgg
      181 ctgtggcggg cgatctcgcg caggttctcg ccgtggtcct gaccgagcag gaggtagtcg
      241 ccgttctcca tcggggcgaa cgtcgagggc atcagcagcg gcatgaagcc gtgcttcgcg
      301 agctccagct cgtggatgat gtccggccgg atcaggctca gggcgtacga gaacgtcgtg
      361 aaccagaagc ccggccggag ctcctcggtg atcgccgcgc cgccgacgag atggcgccgc
      421 tcgaggatca gggtccggag cccgctcttc gcgagatagg cgccgttgac gaggccgttg
      481 tggccgccgc cgatgacgat cgcgtcgtag gtccgatcgt ccgctcggcg gtccggcagg
      541 ttcgtgacgg ggtccgtgcc gttgccggcc gccggaccgc tggtcttcgc catcaggcgg
      601 tcctccgatc ggggttgtag agcggcagcc ggctgaccgc ggcgtcgaaa tactcgtagc
      661 gatggttgac gggcagctcc agcttgaccc gcgagccggg cttcgtccgg tcgaggggaa
      721 tgcgggcgag ggcgatgtga cgctggagca tcggcgagta catcaggctg gtggcgtagc
      781 cgagctgcgt cccgccgtcg tcgtagacgt agtactcgtc ctggatcggg gtgtggtcct
      841 tgggcgggat cagccccgcc tcatcatagt ggcggtcgta gtcctgccag tcgaggacga
      901 gcccggtcag cttccagcgc gacgacttgt cgcggagctc gcgctcgatc gccgcccggc
      961 cgatgaaccg gcgttggtcc gccgcgaggt cacggaccat ccagccgagt ccgagctcga
     1021 tcagggtcga gcggttggcg tcggtccagg cgtagcggct cgatccgaag tcgacatcga
     1081 gcaggagcag cccggcctcg atccgggcca tgtacagcgc gaccagcccg aaggggatca
     1141 cgccgcgccc ggccgacgcg tcgccgaccg cgtcccagac cgcgatcgcc cgatccgccg
     1201 ggatccagag ctcgaagccg aggtcgccgg tatagccggt ccgcgagatc ctcacgggcg
     1261 tgccggcgat cgtcgcatcg gtcaggccga agtacggcag gtcggcgacg gccggcgcca
     1321 ggcaggcgag gatctcgcgc gacttcggcc cctggaccga gaggaccgcc gagtcctcgc
     1381 tcacgtcgtc gatcgtgacc cggtggcggc cgacgaggcc ggcgaagtag gcgaggttcg
     1441 gctcggcgca ggtcaggagg tactcgtcgg cggccgtccg gaagacgacc ccgtcctcga
     1501 ccacgaagcc gcgatcgtcg agccaggccg tgtactggcc gtgaccgacc tggcacgtcc
     1561 ggatgtcgcg ggcgaggata ccggcgaggt atgcctccgc atctggaccg tggatccggt
     1621 acttgtacag cggcgacgag tcgaagagac ccgcactgtt gcggatcgcg aagtactcga
     1681 acttctccga tagctggtat ttctgggcga cgagatagcc cgaccagtgc tcccagagac
     1741 cggagacgtt ggccgcgctc gtccgatcat ggaacggcgt cgtgcggatc atcgagccct
     1801 cgctggtcag gttgtcggcg agccgccgag cgatcgctcg ccgtcacctt cggtcgacgg
     1861 gcgacgtctc cgggacggcc atcggacggt cgcgcatccg cggcggagcg acgcgtcgcg
     1921 tcccgcgtga tgcacccgcg gtcccgccgc gcgcatcatc tcaagcccgt caccccgctc
     1981 tgcgcgaccg gcgccgggga cggtgatcga aacacgggtc gcctatcctg tggccgtggc
     2041 gctctaccgc atcgacgccc cgggaccgct gaaccgcccc gccctcgtcg tggcgctcga
     2101 cggctgggtc gacgccggga gcgccgcgac gtcggcggcc ggacagatcg cgacgggtgg
     2161 gcaggtcgtc gcgtccttcg acgccgatcg gctgttcga
//
LOCUS       DATAIW010000462        10612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_255062, whole genome shotgun sequence.
ACCESSION   DATAIW010000462 DATAIW010000000
VERSION     DATAIW010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255062"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VHM48_13810"
     CDS             complement(<1..805)
                     /locus_tag="VHM48_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756538.1"
                     /translation="MFTDRTGPGGGWRRLGIFIGSAAIVVSACGGSTATPSVVPASAA
                     PPASAPATTSSAPSAAPSDAGYTGPDATISYSIWGDPQEIKNQQAIVDAFHVANPKIT
                     VKVTVSDWEPYWDKLQTSIAGGDAPDVFAMDGPLFPDYQSRDVLLDLKPFIDRDGYDL
                     GNLADQAEADFTTAGGQFGVPRDLNVVALYYNKKMFDAAGISYPDDTWDWAKLVEVAK
                     QLTIRSGSKTTQWGFYTETTDMENYWSELVWQNGGDIVSADHKTSLVGSA"
     gene            complement(821..2140)
                     /gene="melA"
                     /locus_tag="VHM48_13815"
     CDS             complement(821..2140)
                     /gene="melA"
                     /locus_tag="VHM48_13815"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX2756539.1"
                     /translation="MPRIVLVGAGSVEFTRNLLGDILTAPALAGSQIALHDIDPERLA
                     TAERMARWTAATLGASPTISAHLDRREALAGADFVVNTIQVGGARATQADFDVPARFG
                     LRYTIADTVGVGGVFRGLRTIPVVLGIARDMEEICPDAWLLNYTNPLAILVRAVSEAT
                     SIRVVGLCHSVFWTVDRLAGYLGIPREEVDSATAGLNHHAFILRLEHRGRDLYPDLRA
                     FIEAGRAPDDDLVRAELFGRLGFYPTESSEHHAEYNPWYIGKSDAAGDAVERFHIPIG
                     EYLDRVAHNLDEYAETRRMLDAGEPFEIERSGEYAATIAEAMTTGAPARIVVNAMNRG
                     ALIPNLDRDVCVEVPALVDGLGVHPVAMGLLPLHLAAYIRGAADMQGLTVRAALDRDR
                     AAVGHAVMTDPIVQSRLTLADAWRLTDDMIEAEAEWLPAWLGGSAAG"
     gene            2276..3343
                     /locus_tag="VHM48_13820"
     CDS             2276..3343
                     /locus_tag="VHM48_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2756540.1"
                     /translation="MRAGGRATDPQTRERIVRAAVVQAAPVAFDRERTLNRVAELTAA
                     AAADRAQLVVFPEAFVSGYPKGADFGAVVGSRSREGREWFRRYHESGLDVPGPAVDRL
                     GDIARSNAVHLVIGVIERDRGTLYCTVLFLGPDGDLLGKHRKLMPTAMERLIWGFGDG
                     STLTVLPTPLGRIGAVICWENYMPQLRLAMYGQGIELYCAPTVDDRETWLPTMRHIAL
                     EGRCFVLSANQFARRSDYPTDYPVETDHPDAVLIAGGSCIVDPFGQVLAGPARDGEAI
                     LTADLDLGEIARGTFDLDVVGHYGRPDVFQLVVNDGVQTTVSWASAETGQAGAESDPS
                     AVAAPGPDRTQSTSLPNVPPG"
     gene            3537..5201
                     /locus_tag="VHM48_13825"
     CDS             3537..5201
                     /locus_tag="VHM48_13825"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2756541.1"
                     /translation="MNVVEPVGTARRALDELIARASATQTTEPGSAVDPATRPRIDLA
                     FRPGLDLEPRSPRWIKVIDQTRCIGCHACTTACKSENEVPIGVTRTYVKAVDVGTFPN
                     ARRAFQVTRCNQCSDAPCVAACPTSAMFRRPDGIVDFDKAACIGCKACIAACPYDAIF
                     INPEDHSAEKCNFCAHRIDGGLEPACVVVCPTQAILVGDLNDPDSAVARIVSREPVAV
                     RRPEKETRPGLFYKGAHQATLDPLAARRPAGGTFMWSEQGSGEQIVNAGHPGAWNSSA
                     AAVLAYDVPHRAPWDWRVSLYTWTKGIAAGAYIIPLFLVLAGIIAPSSPLWTWVAPVV
                     AGAFLALTGLLLLLDLEHPLRFVYIFLRPQMRSWLVRGAVFITGFGALLALHFVAGLA
                     GRVDLTRWLAIAGLPLAALTAVYTAYLFAQAKARDLWQSPLLPPHLLVQAALAGSAVL
                     LVAAFVFEPGAVATLEVWLAGATAVHLLLVSGEITLTHPTAHARLAAHEMFAGRFADF
                     FWIGIALVAGGLLAPVLGPIAAVAALVGLLAHEHAYVQAGQSVPLA"
     gene            5212..8193
                     /locus_tag="VHM48_13830"
     CDS             5212..8193
                     /locus_tag="VHM48_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2756542.1"
                     /translation="MDPRLDEIAERVSAARQAAAARGETFYPGPSRIHLAAFPPKERW
                     DNWVELDSRAWPKRVEKRFMLVPTTCFNCESACGLLAYVDRDTLQVRKFEGNPEHPGS
                     RGRNCAKGPATLNQVTDPDRILYPLKRVGARGEGRWERVAWDEALDDIAGRIRTAIVE
                     ERPNEVMYHVGRPGEDGFTERVLASWGVDGHNSHTNVCSSGGRAGYHYWMGIDRPSPD
                     HANAKVILLISAHLESGHYFNPHAQRVMEAKQAGAKLIVFDTRLSNTATHADLWLSPW
                     PGSEAAILLAVANHLIATGRHDREFIRRWWNWEEYLAAEEPDRKPTFARFERRLATLY
                     ADYTFEFAAAESGVAAATIAQVAETVAEAGTALSTHTWRSAAAGNLGGWQVSRTLFLL
                     NCLMGAVATPGGTYPNAWNKFVPVPIHTPQHPHTWNELTWPVEYPLALNELSFLLPHF
                     LKEGRGKLDVYFTRVYNPVWTNPDGFSWIEALTDEALVGLHVALTPTWNETSFFADYI
                     LPMGLGSERHDVHSYEQYDGQWIGFRQPVLRAARERTGETIDDTRSVNPGEVWEENEF
                     WIEMSWRIDPDGRLGIRRFHESKEHPGEKLSVDEYYGWMFDHSIPGLPARAAAEGLSP
                     LQFMRRYGAFEISKGVGAVHEQPVPTDELVDTHTDRFGRVYTAAPKPAAVNLAPTGSP
                     EPDEDGRRPVGVLVDGTVRRGFPTPSGKLEFYSAMLADWGWPEVAIPAYMRSHVHPSN
                     LAADEMVLISTFRLPVQIHTRSGNAKWLDEIAHTNPLWLHPLDAARMGIGEGDLVRVE
                     TEIGYFVVKAWVTEGIRPGVVACSHHMGRWKLGDQGQRSVMATVALDHEGDGWDMSRK
                     KGGGAFESADPDTLRIWWTDVGVHQNLTFPVHPDPISGQHCWHQAVRVRKAEPTDAYG
                     DIHVDTARSKEVYRHWLELTKPADRFSPDGSRRPFWLMRPLKPARAVYDLPTAAADPG
                     TAANVAGSQRNRKDKRKARTR"
     gene            8190..9074
                     /locus_tag="VHM48_13835"
     CDS             8190..9074
                     /locus_tag="VHM48_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX2756543.1"
                     /translation="MRARADLFRALGALCEPPEPGHIAIAAAIGLPGGPASGDYAEVF
                     LFGAYPYASVYLGEEGMLGGEARDRVAGFWRALGIRPPTEPDHIAALLGLYATLIELE
                     ESEPDKARAALARSSRKALLWEHLLSWLPAYLDKLMEIAPPYYADWARLLGDALIDEA
                     ADVGGQTELPLQLRAAPGLPETDAPSADWVQALLSAVRTGVVLTRFDLRRGARELGIG
                     ARVAERAYMLRSMLEQDAPATTSWLAAEASRWAIRHQEKEATLGRVAVFWRTRAEASN
                     AALRSLAQAVPAAARDDR"
     gene            9064..10074
                     /locus_tag="VHM48_13840"
     CDS             9064..10074
                     /locus_tag="VHM48_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2756544.1"
                     /translation="MIVEGIDLGRELEPDLGIDPCPASPPPDPAGPPGDRPLPNRDPA
                     GHLRSGPAARISALLAHPWLPLVATWLLLVFLAARGDSLVGLLIGLAFAGAFLSGLVG
                     VGGAIVMIPLLLYAPPLIGLQTLGIKTVAGITIVQVTAAAFAGLLGHRQGIHRDLFMV
                     LGPSMVVASFLGGLTSRFFEPVVLEAVFAAMATLAAAMMLLLRRRTAAEIDGPTAFSR
                     PAAIGTGVGVGFAAGLLGAGGAFFLIPVMLYGLRIPVRSTVGTSLAVVAVSALAALLG
                     KAVTGQIDWILAAALVVGALPGARLGSYVSGRTRTDRLVVVLGLVISLVALRMWVGIV
                     SA"
     gene            complement(10119..10478)
                     /locus_tag="VHM48_13845"
     CDS             complement(10119..10478)
                     /locus_tag="VHM48_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756545.1"
                     /translation="MSGAHAVVGQVAVILTMIATIWAIAMAITHRPLGSLFLGNLVWV
                     AIAVVIAAALGAVTALAEGPPRDVLHVVYGVLALGTLPGAAIVATGRSVRQQAIVAAV
                     AATILVILLFRLVQTGS"
BASE COUNT         1533 a         3773 c         3629 g         1677 t
ORIGIN      
        1 cggcgctgcc gacgaggctc gtcttgtgat ccgccgagac gatgtccccg ccgttctgcc
       61 agacgagctc cgaccagtag ttctccatgt cggtcgtctc ggtgtagaag ccccactggg
      121 tcgtcttgct gcccgaccgg atcgtcagct gcttggcgac ctcgacgagc ttcgcccagt
      181 cccaggtgtc gtcgggatag gagatgcccg ccgcatcgaa catcttcttg ttgtagtaca
      241 aggcgacgac gttcaggtcg cgtggcacgc cgaactgccc gccggccgtg gtgaaatcgg
      301 cctccgcctg gtcggccagg ttgcccaggt cgtagccatc ccggtcgatg aacggcttca
      361 ggtcgagcag gacatcgcgg ctctggtagt cggggaagag cgggccgtcc atcgcgaaga
      421 cgtcgggggc gtcgccgccg gcgatcgagg tctggagctt gtcccagtac ggctcccagt
      481 cggagacggt caccttcacg gtgatcttcg ggttggccac gtggaacgcg tcgacgatcg
      541 cctgctggtt cttgatctcc tgggggtcgc cccagatcga gtagctgatc gtcgcgtccg
      601 ggccggtgta gccggcatcg ctcggcgcgg ccgacggcgc gctgctggtc gtggccggcg
      661 cgctcgccgg cggcgcggcg ctcgcgggca cgacggatgg ggtggccgtc gagccgccac
      721 acgccgaaac gacgatcgct gcgcttccga tgaagatgcc gaggcgacgc catccgccgc
      781 cgggtcccgt ccgatccgtg aacatcgcgt cgctcctcct tcaaccggct gcggatccgc
      841 cgagccacgc gggcagccac tccgcctcgg cttcgatcat gtcgtctgtg aggcgccacg
      901 cgtcggccag ggtgagtcgg ctctggacga tgggatcggt catgaccgcg tggccgacgg
      961 cggcacgatc gcgatcgagc gcggcccgga ccgtcaggcc ctgcatgtcc gccgctccac
     1021 ggatgtaggc cgcgaggtgg agcggcagca ggcccattgc gaccgggtgg accccgagcc
     1081 cgtcgacgag cgcgggcacc tccacgcaga cgtcccggtc gaggttcggg atgagggccc
     1141 cgcggttcat cgcgttgacg acgatccggg ccggcgcacc cgtcgtcatc gcctcggcga
     1201 tcgtcgcggc gtactcgcca ctgcgctcga tctcgaacgg ctccccggca tccagcatgc
     1261 gcctggtctc ggcgtactcg tcgaggttgt gggcgacccg atcgaggtac tccccgatcg
     1321 ggatgtggaa gcgctcgacc gcatcgccgg cggcgtcgct cttgccgatg taccacgggt
     1381 tgtactcggc gtggtgctcg gaggattcgg tcgggtagaa cccgagccgg ccgaacagct
     1441 ccgctcggac caggtcgtcg tccggggcac ggcccgcttc gatgaacgcg cggaggtccg
     1501 ggtagagatc cctgccgcgg tgctcgaggc ggaggatgaa cgcgtggtga ttgagccccg
     1561 ccgtggccga gtcgacctcc tcacgcggga tgcccaggta gccggcgagc cgatcgaccg
     1621 tccagaagac agagtggcag agcccgacga cccggatgga ggtcgcctcg gacacggccc
     1681 ggacgaggat cgcgagcgga ttcgtgtagt tgaggagcca ggcgtcgggg cagatctcct
     1741 ccatgtcccg agcgatcccg aggacaaccg ggatcgtccg caggccgcgg aacacgccgc
     1801 cgacgccgac cgtatcggcg atcgtgtagc gcagcccgaa cctggccggt acgtcgaagt
     1861 cggcctgggt cgcccgggcc ccgccgacct ggatggtgtt gaccacgaag tccgcaccgg
     1921 cgagcgcctc ccggcgatcg aggtgggccg agatcgtcgg gctcgcgccg agggtcgcgg
     1981 cggtccagcg cgccatccgc tccgccgtcg cgaggcgctc cggatcgatg tcgtggagtg
     2041 cgatctgcga ccctgccagg gcgggcgccg tcaggatgtc gccgagcagg ttccgcgtga
     2101 actcgacgct cccggcgccg acgaggacga tcctgggcat ggctctcctg cacaagacgg
     2161 cgcgggcatc aggacgtccg gacgtcctga tgtcaaggta acaggtcggg cctgccgttt
     2221 gcaacccgcc gcgttacgct ccagccctgc gcaatcgcgc gaacgaggat ggccaatgcg
     2281 tgccgggggc cgagccacgg acccacagac tcgcgagcga atcgttcggg cggccgtcgt
     2341 ccaggcggcc ccggtggcgt tcgaccgcga gcggacgctc aatcgggtcg ccgagctcac
     2401 cgctgcagcc gccgccgacc gtgcccagct cgtcgtgttc cccgaggcgt tcgtctcggg
     2461 ctatccgaag ggtgccgact tcggcgcggt cgtcggcagc aggtcgcgcg aggggcgcga
     2521 gtggttccgt cgctaccacg agagcggcct ggacgtcccg ggacccgctg tcgaccggct
     2581 tggcgacatc gcccgcagca acgccgttca cctcgtcatc ggcgtgatcg agcgcgatcg
     2641 cggcaccctg tactgcacgg tcctgttcct gggcccggat ggcgacctgc tcggcaagca
     2701 ccgcaagctc atgccgaccg cgatggagcg cctcatctgg ggcttcggcg acggctcgac
     2761 gctgacggtc ctgccgacgc cgctcggtcg gatcggcgcg gtcatctgct gggagaacta
     2821 catgccccag ctgcggctcg cgatgtacgg gcaggggatc gagctgtact gcgcgccgac
     2881 cgtcgacgat cgcgagacgt ggctgccgac gatgcgccac atcgcgctcg aggggcgctg
     2941 tttcgtgctc tcggcgaacc agttcgcccg cagatccgac tatccgaccg actatccggt
     3001 cgagaccgac caccccgacg cggtcctcat cgccggcggg agctgcatcg tcgatccatt
     3061 cggccaggtc ctcgccggtc cggcgcggga cggcgaagcc atcctcaccg ccgacctcga
     3121 cctcggcgag attgcccgcg gtacgttcga tctggacgtc gtcggccact acggacggcc
     3181 cgacgtattc cagctcgtcg tcaatgacgg ggttcagacg accgtgtcgt gggcgtccgc
     3241 ggagactggc caggcggggg ccgaatccga tccctccgcg gttgccgcac ccggacccga
     3301 ccgcacgcag tcgacgagtt tgccgaacgt gcccccggga tgaagatcgc ggtggtcgga
     3361 accgatgggc tcggccgctc ccgaccgatg cgcgaacgtc gacaatcggc gagtcaattg
     3421 agcgatcctt ggttgcgtga cgaattggcc gccgcttccg ctccagtccg cgacgctgtt
     3481 gcccgtcaca tcgggcgcgc ggtcctgagc gccggacggc aggcgaggac gcgcacatga
     3541 acgtcgtcga accggttggc acagcgcgcc gcgcccttga cgagttgatc gcgcgcgcgt
     3601 cggccaccca gacgaccgag ccgggctcgg ccgtcgaccc ggcaactcgg ccgcggatcg
     3661 acctcgcctt ccgcccgggc cttgacctcg agccccggtc gccacgctgg atcaaggtca
     3721 tcgaccagac ccgctgcatc ggctgtcacg cctgcacaac cgcctgcaag tcggagaacg
     3781 aggttccgat cggggtgacc cggacgtacg tcaaagcggt cgacgtcggg acgtttccca
     3841 acgcgcgacg ggccttccag gtcacccgct gcaaccagtg ctcggacgcc ccatgcgtcg
     3901 cggcctgccc cacgtccgcg atgttccgac ggccggacgg gatcgtcgac ttcgacaagg
     3961 ctgcctgcat cggctgcaag gcctgcatcg cggcctgccc gtacgacgcg atcttcatca
     4021 accccgagga ccattcggcc gagaagtgca atttctgcgc tcatcggatc gacggtgggc
     4081 tcgagcctgc ctgcgtcgtc gtgtgcccga cccaggcgat cctcgtcggc gacctcaacg
     4141 atcccgattc ggcggttgcc cggatcgtca gccgcgagcc cgtcgccgtc cgccggcccg
     4201 agaaggagac ccggccgggc ctcttctaca agggtgccca ccaggcgacg ctggacccgc
     4261 tggccgcccg ccgtccggcc ggcggcacgt tcatgtggag cgagcagggc tcgggtgagc
     4321 agatcgtgaa cgctggccac ccgggcgcgt ggaacagcag tgccgccgcc gtcctcgcct
     4381 acgacgtccc ccatcgggcg ccgtgggact ggcgggtcag cctctacaca tggaccaagg
     4441 gcatcgccgc cggcgcctac atcatcccgc tgttcctggt cctcgccggg atcatcgccc
     4501 cgtcgagtcc cctctggacg tgggtcgcgc ccgtcgtcgc cggcgccttc ctggccctga
     4561 ccggcctgct gctgctgctc gacctcgagc atccgctccg gttcgtctac atcttcctcc
     4621 ggccacagat gcgcagctgg ctcgtccgag gcgcggtctt catcacgggc ttcggcgccc
     4681 ttctcgcgct ccatttcgtc gccggcctgg caggccgggt ggacctgacc cgttggctgg
     4741 cgatcgccgg cctgccactg gccgccttga ccgccgtcta cacggcctat ctcttcgccc
     4801 aggcgaaggc gcgcgacctc tggcagagtc cgctccttcc gccgcacctc ctcgtccagg
     4861 cggcgctcgc cgggtcggcg gtcctcctgg tcgccgcatt cgtcttcgag ccgggcgcgg
     4921 tcgcgacgct ggaggtctgg ctggcgggcg cgacggccgt ccatctcctg ctcgtcagcg
     4981 gggagatcac cctgacccat ccgacggccc acgcccggct ggcggcccac gagatgttcg
     5041 cggggaggtt cgccgacttc ttctggatcg ggatcgccct cgtcgccggc gggctgctgg
     5101 cgcccgtcct cggtccgatc gcggcggtcg ccgccctggt cggacttctc gcccatgaac
     5161 acgcgtacgt ccaggcaggc cagtccgtcc cgctggccta ggaggagatc gatggatccg
     5221 aggctcgatg agatcgccga acgggtgagc gcggcccgtc aggcggccgc tgcccggggc
     5281 gagaccttct atccgggtcc gagtcggatt cacctcgcgg cgttcccgcc aaaggaacgc
     5341 tgggacaact gggtcgaact cgattcgcgc gcctggccga agcgcgtcga gaagcgcttc
     5401 atgctcgtcc cgacgacctg cttcaactgc gagtcggcgt gcggcctgct ggcctacgtc
     5461 gatcgcgaca cgctccaggt gcgcaagttc gagggtaatc ccgagcaccc gggctcgcgc
     5521 ggacggaact gcgccaaggg cccggcgacg ctcaaccagg tcaccgaccc cgatcggatc
     5581 ctgtacccgc tcaagcgcgt cggtgcgcgg ggcgagggcc gttgggagcg cgtcgcctgg
     5641 gacgaggccc tcgacgatat cgccggccgg atccggacgg cgatcgtcga ggagcgcccg
     5701 aacgaggtga tgtaccacgt cggccggccc ggcgaggacg gcttcaccga gcgcgtcctc
     5761 gcctcgtggg gcgtcgacgg ccacaactcg cacacgaatg tctgctcgtc gggtgggcgg
     5821 gccggctacc actactggat gggcatcgac cggccgagcc cggatcatgc caatgccaag
     5881 gtcatcctgc tgatcagcgc ccatctcgag tcaggccact acttcaaccc ccacgcccaa
     5941 cgggtgatgg aggcgaagca agcgggcgcc aagctcatcg tcttcgacac acggctgtcc
     6001 aacaccgcga cccacgccga cctctggctt tcgccgtggc ccggatccga ggcggccatc
     6061 ctcctcgcgg tcgccaacca cctgatcgcg accggtcgcc atgaccggga gttcatccgt
     6121 cgctggtgga actgggagga gtacctcgcc gccgaggaac cggaccggaa gccgacgttc
     6181 gcccggttcg agcggcgcct cgccaccctc tacgccgact acacgttcga gttcgcggcc
     6241 gcagaatcgg gcgtcgccgc ggcgacgatc gcccaggttg ccgagacggt ggccgaggcg
     6301 gggaccgccc tttcgaccca cacctggcgg tcggcggccg cgggcaacct cggcggctgg
     6361 caggtgtcgc ggacgctgtt cctcctcaac tgcctgatgg gcgcggtggc gacgccgggt
     6421 ggcacgtacc cgaacgcctg gaacaagttc gttccggtgc cgatccacac gccgcagcat
     6481 ccccatacct ggaacgagct gacctggccg gtcgagtacc cccttgccct caacgagctc
     6541 tcgttcctcc tgcctcactt cctgaaggag ggcaggggca agctcgacgt ctacttcacc
     6601 cgcgtctaca acccggtctg gacgaacccg gacggattca gctggatcga ggccctgacc
     6661 gacgaggcgc tcgtcgggct tcatgtcgca ctgaccccga catggaacga gacctcgttc
     6721 ttcgccgact acatcctgcc gatgggcctc ggctccgagc gtcacgacgt ccacagctac
     6781 gagcagtacg acggccagtg gatcgggttc cgccagcccg tcctgcgggc cgcccgcgag
     6841 cgaacgggcg agacgatcga cgacacgcgg tcggtcaacc cgggcgaggt ctgggaggag
     6901 aacgaattct ggatcgagat gtcgtggcgg atcgacccgg atgggcgcct cggcatccgc
     6961 cgcttccacg agtcgaagga gcatcccggc gagaagctgt cggtcgacga gtactacggc
     7021 tggatgttcg atcactcgat tccgggcctg cccgcacggg cggccgccga ggggctcagc
     7081 ccactccagt tcatgcgccg ctatggagcg ttcgagatca gcaagggtgt cggggcggtt
     7141 catgagcagc cggtcccgac cgatgagctc gtcgacaccc acaccgaccg gttcggacgg
     7201 gtgtacaccg cggccccgaa gccggcggca gtcaatctcg ctcccaccgg cagcccggag
     7261 ccggacgaag acggccgccg tccggtcggg gtcctggtcg atgggaccgt ccgccgcggc
     7321 ttcccgaccc cgagtggcaa gctcgagttc tattccgcga tgctggccga ctggggatgg
     7381 ccggaggtcg cgatcccggc gtacatgcgc agtcacgtcc acccgtcgaa cctcgccgcc
     7441 gacgagatgg tcctgatctc gacctttcgc ctgccggtcc agatccacac gcgaagcggc
     7501 aacgcgaagt ggctcgacga gatcgcccac acgaacccgc tctggctgca tccgctcgat
     7561 gctgcccgga tgggcatcgg cgagggggac ctcgtccggg tcgagaccga gatcggctat
     7621 ttcgtcgtga aggcctgggt gaccgagggg atccggcccg gcgtggtggc ctgcagccac
     7681 cacatgggcc gctggaagtt gggcgaccag gggcagcgga gcgtcatggc cacggtcgcc
     7741 ctcgaccacg agggtgacgg ctgggacatg tcccgcaaga agggtggcgg agccttcgag
     7801 agcgccgatc ccgacacgct ccggatctgg tggacggatg tcggggtcca tcagaacctc
     7861 acgttcccgg tccaccctga cccgatctcg ggtcagcact gctggcacca ggcggtccgc
     7921 gtgcggaagg ctgagccgac cgacgcctac ggcgacatcc acgtcgacac ggccaggtcg
     7981 aaggaggtct accggcactg gctcgagttg accaagcccg ccgaccggtt ctcgccggac
     8041 ggctcacgtc ggccgttctg gctgatgcgc ccgctcaagc ccgcccgcgc cgtctacgac
     8101 ttgccgacgg ccgctgctga cccgggcacg gccgcgaacg tggcggggtc acagcgcaac
     8161 cggaaggaca aacggaaggc ccgcacgaga tgagggcgcg ggcggacctg ttccgcgcgc
     8221 tcggagcgct gtgcgaaccg ccagaacccg gccatatcgc gatcgccgcg gcgatcgggc
     8281 ttcccggagg gccggccagc ggtgattacg ccgaggtctt cctcttcgga gcttacccgt
     8341 atgcctcggt ctatctcggc gaggagggga tgctcggtgg cgaggcccgc gatcgggtcg
     8401 ccggcttctg gcgtgccctc ggcatccggc cgccgaccga gccggaccac atcgccgcgc
     8461 tgctgggcct ctacgcgacc ctgatcgagc tcgaagagtc ggagcccgac aaggcgcgag
     8521 cggccctcgc ccgttcgtcg cgcaaggcgt tgctctggga gcatctcctg agctggctgc
     8581 ccgcgtacct cgacaagctc atggagatcg cgccgcccta ctacgcggac tgggccaggc
     8641 tccttggcga cgccctcatc gacgaggccg cagacgtcgg cggccagacg gagctccccc
     8701 tccagctgcg ggcggcgccc ggactgcctg agaccgatgc cccaagcgcc gactgggtcc
     8761 aggcattgct cagcgccgtc cgaaccggcg tggtcctcac gcggttcgat ctgcggcgcg
     8821 gggcccgcga gcttgggatc ggagcgcggg tcgcggagcg cgcctacatg ctccgatcga
     8881 tgctcgagca ggacgcgccg gcgaccacgt cgtggcttgc cgccgaggcg agccgatggg
     8941 ccatcaggca ccaggagaag gaagccaccc tcggtcgcgt ggccgtgttc tggcggacac
     9001 gagccgaggc gagcaacgcc gccctgcgtt cactggcgca ggccgtgccg gcggcggctc
     9061 gcgatgatcg ttgaggggat cgacctcggc cgtgagctcg agcccgacct tggaatcgat
     9121 ccttgccccg ccagcccgcc accagacccg gccggacccc ctggggatcg cccgcttccg
     9181 aaccgtgacc cggccggtca cctgcggtcg ggcccagcgg cccggatctc ggcgctgctc
     9241 gcgcacccgt ggctgccgct cgttgcgacc tggctcctgc tcgtcttcct cgccgcgcgc
     9301 ggggactcgc tcgtcggcct cctgatcggc ttggccttcg cgggcgcatt cctgtccggg
     9361 ctcgtgggtg tcggcggcgc gatcgtgatg atcccgctcc ttctctacgc cccgccattg
     9421 atcggcctcc agaccctcgg catcaagacg gtcgcgggga tcacgatcgt ccaggtgacg
     9481 gcggccgcat tcgcggggct cctcggccac cggcagggca tccatcggga cctgttcatg
     9541 gtgctcgggc cgagcatggt cgtcgcctcg ttcctgggtg ggttgacctc ccggttcttc
     9601 gagccggtcg tcctcgaggc cgtattcgcc gcgatggcga cgctcgccgc ggcgatgatg
     9661 ctgctccttc gccggcgcac cgcggcggag atcgacggcc cgaccgcgtt cagccggccg
     9721 gccgcgatcg gaacgggcgt gggagtgggg ttcgctgccg gattgctcgg cgctggtggc
     9781 gcgttcttcc tcatcccggt gatgctctac gggctcagga tcccggtccg ttcgacggtc
     9841 gggacgtccc tcgcggtcgt tgccgtatcc gcgctcgccg cccttctggg caaggccgtg
     9901 acggggcaga tcgactggat cctcgccgcg gcgctcgtgg tcggcgctct gccgggtgcc
     9961 cgtctcggct cgtacgtctc tggccggacg cggacggatc gactcgtcgt cgtgctgggt
    10021 cttgtcatct cgctcgtcgc cctgcggatg tgggtcggca tcgtttcggc ctagtgcggg
    10081 ccgcggaacg cgccctggta cacccgcaag ggcaccagct acgagccggt ctgaacgagg
    10141 cggaacagga ggatcaccag gatcgtcgcg gccacggccg cgacgatcgc ctgctgtcgt
    10201 acggaccgcc cggtcgcaac gatcgccgcc ccgggcagcg tcccgagcgc aagcacgcca
    10261 tacacgacgt gcagcacgtc gcgcggcggc ccttccgcga gcgctgtcac cgccccgagg
    10321 gcagccgcga tcacgacggc gatggcaacc cagacgaggt tgccgaggaa cagcgagccg
    10381 agtggccggt gcgtgatcgc catcgcgatc gcccagatcg tcgcgatcat cgtgaggatc
    10441 acggcgacct gaccgaccac ggcgtgggct ccgctcattg gagcaccctc tcaactgcca
    10501 cgatgagggt ggccctcggg tcctgccggg gtgacgagtc cgctcgcttg tcgcgtgacg
    10561 accagcatga gatctctcgc ccccggtggc ccgggtgacc cggatcggcc ac
//
LOCUS       DATAIW010000463         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_226889, whole genome shotgun sequence.
ACCESSION   DATAIW010000463 DATAIW010000000
VERSION     DATAIW010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226889"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1198)
                     /locus_tag="VHM48_13850"
     CDS             complement(239..1198)
                     /locus_tag="VHM48_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2756546.1"
                     /translation="MARAMWKGAIQFGLVTIPVKLYLATESDYTIRFNMLHAEDHSRI
                     QMKTWCPVEDKPISRADTVKGYEYAPGEYVVITDEDLEKVPLKTIRSIEIEQFTKADD
                     EEAANTRFVKQAYYVEPDKIGRKPFYLLRSVLQEEGLKAICKVVIKDREALAALDPFA
                     DTMLLTTLHWPDEIRSTAELDLPAEDYEFKPAELAMARQLVSAMTGEFDPAEYKDEYR
                     DALMGVIESKVEGHEVKAPEPVEEGGKLIDLMAALEASVNAAKAARMADKPVSVAEAK
                     KARAGKAAGEKASTARGKAAAKADEAESEADAAPAKSRKRKTA"
     gene            complement(1312..3756)
                     /locus_tag="VHM48_13855"
     CDS             complement(1312..3756)
                     /locus_tag="VHM48_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2756547.1"
                     /translation="MRRSLAALAAAALLLSSVGSATGAGPDPSASSAPIGEPTPSVDP
                     PPTADPSPAAIPDPGSETDPATAATTRPSHFGEPATAPIVETTGPSRKDPATTRAARA
                     ATDPTDRWIVVLKPGSNVDAAIERQGKRIGFVTDRTYRNSLRGYSATLDRAQVTALSH
                     DASVAMIVADEKIQAESQALPTGITRIGANRSTAAKIDGVDVRVDADVAIVDTGIAKV
                     ADLNVAGGYNCSTSNRAAWRDVYGHGTHVAGTVGAIDNGIGVVGVAPGVRLWAVKILD
                     DTGDGLLSWYVCGLDWIAAQHDPVDPTRPRFEAVNMSVAKRGSDDRHCGAWNKDILHA
                     AICRLVASGVTVVAAAGNDSSSAAARVPAAYNEVITVSALADTDGKPGAIGGHRCFSW
                     GTYDNDDSFADFSNRGSDVDLIAPGKCIWSTIPGGYAYSSGTSMAAPHVTGAVALLKA
                     SRPALTPVEVREALIYLGTYNWKTWTDPDGHPDKLLDVSRIGPRGDFSVAAGPDVTIG
                     EAGGSARFPITITRTATSFERIRLAVGDLPAGATASLDATSLYGFAGVASTLTVTFST
                     GTPAGSYPVSVTADEHGNTHTAIATVVVEVDVPTAQPPATAALARAQLGTTSLATRTS
                     WPAATDPTTGIAGYELESSVDGGAWVATTATSATVRSTLGSQSIGHAYRYRVRAQDTV
                     GNWSAWANGPSVTGVLVQDRSSAVSYSGTWTKASYTYASGGSTTWAVSKGARARTTFS
                     GRGIALVAPTSSTRGSAAIYIDGVYRTTISFRSAANHGRVVLYSTSFATLATHTIELR
                     LSGNGRVDLDTFVILR"
BASE COUNT          557 a         1322 c         1445 g          663 t
ORIGIN      
        1 cggatttgcg cgtcgtgctc gaatgcaaga tatccctggc cagactcttg ggcgcggccc
       61 agagcgcgcg acgcgcgccg aatgcctccc cgcggaccgg aatcagccct cgaaggctga
      121 caaatgagtc agccggctcg atcggcacac cgatcttgcc gaatcggcag cgcgatctct
      181 gccgagtctg ccggcgaata tctggcctgg tgcgcgcccg aggtggagcg ccggagcgtc
      241 aggcggtctt gcgcttgcgg ctcttggcgg gggcggcgtc agcctccgac tcggcctcgt
      301 cggccttggc cgcggccttg ccccgcgccg tcgatgcctt ctcgccagcc gccttgccgg
      361 cgcgcgcctt ctttgcctcg gcgacgctga ccggcttgtc ggccatgcgg gcagccttcg
      421 cggcgttgac cgacgcctcg agcgcggcca tcaggtcgat cagcttgccg ccttcctcga
      481 cgggctccgg tgccttgacc tcgtggcctt cgaccttcga ctcgatgacc cccatcaggg
      541 cgtcgcggta ctcgtccttg tactcggccg ggtcgaattc gccggtcatc gccgacacga
      601 gctggcgggc catcgcgagc tcggcgggtt tgaactcgta gtcctcggcg ggcaggtcga
      661 gctcggcggt cgagcggatc tcgtcgggcc agtggagcgt cgtgaggagc atcgtgtcgg
      721 cgaacgggtc gagcgccgcg agggcctcgc ggtccttgat cacgaccttg cagatcgcct
      781 tgaggccctc ctcctggagg accgagcgaa gcagatagaa gggcttccgg ccgatcttgt
      841 cgggctcgac gtagtaggcc tgcttgacga accgggtatt cgcggcctcc tcgtcgtcgg
      901 ccttggtgaa ctgctcgatc tcgatcgagc ggatcgtctt gagcgggact ttctcgaggt
      961 cctcgtcggt gatgacaacg tattcaccgg gtgcgtattc gtagcccttg acggtgtcgg
     1021 cgcgcgagat cggcttgtcc tcgaccggac accacgtctt catctggatc cgcgaatgat
     1081 cctcggcgtg gagcatgttg aagcggatcg tgtagtccga ctcggtggcc aggtagagct
     1141 tcaccgggat ggtcaccagc ccgaactgga tcgctccctt ccacatcgcg cgcgccacgg
     1201 caggtcctcg tacggttgtc gagcgggtcg tgacgagcgt tccgtcacag ccggttccgc
     1261 atgatagcgc gggcgtgcaa cgggtccgcc tcgcgccaac ccgtcagctc gtcaacgcag
     1321 gatgacgaac gtgtcgaggt cgacccggcc gttgccgctg aggcggagct cgatcgtgtg
     1381 ggtcgcgagc gtggcgaacg acgtcgaata cagcacgacc cggccgtggt tcgcggcgct
     1441 ccggaaactg atcgtggtcc ggtagacgcc gtcgatgtag atcgccgccg agccgcgggt
     1501 gctgctcgtt ggggcgacga gggcgatccc gcggccgctg aaggtcgtcc gagcccgggc
     1561 acccttcgac accgcccagg tcgtcgaacc gcccgaggca tacgtgtagc tcgccttcgt
     1621 ccacgtcccg ctgtagctga cggcgctcga gcggtcctgg acgaggacac cggtgacgct
     1681 cggcccgttg gcccaggcgc tccagttgcc gacggtgtcc tgggcccgga cgcgatagcg
     1741 ataggcgtga ccgatggact ggctgccgag cgtgctgcgg acggtcgcgc tggtggctgt
     1801 ggtcgcgacc caggccccgc cgtcgacgct cgattccagc tcgtagccgg cgatgccggt
     1861 cgtgggatcg gtggcggcgg gccagctggt cctcgtcgcg agcgacgtgg tcccgagctg
     1921 ggccctggcc agggcagcgg tcgccggcgg ttgggcggtc ggcacgtcga cctcgacgac
     1981 gaccgtggcg atcgcggtgt gggtgttgcc gtgctcgtcg gcggtgacgg agaccgggta
     2041 ggaacccgcc ggcgtaccgg tcgagaaggt cacggtcagc gtcgaggcga cgccggcaaa
     2101 gccgtacagg ctcgtcgcat cgagcgatgc ggtggcgccg gcgggcagat caccgaccgc
     2161 gagccggatc cgctcgaacg aggtggccgt gcgagtgatc gtgatcggga agcgagcgga
     2221 cccgccggcc tcgccgatcg tcacatcggg accggccgcg accgagaagt cgccgcgcgg
     2281 cccgatccgg gagacgtcga gaagcttatc gggatggccg tccgggtccg tccaggtctt
     2341 ccagttgtag gtgccgaggt agatcagggc ttcccgcacc tcgaccgggg tcaacgccgg
     2401 gcggctcgcc ttgagaagcg ccaccgcgcc ggtcacgtgc ggagccgcca tcgacgtccc
     2461 cgagctgtac gcgtagccgc ccgggatcgt cgaccagatg catttgcccg gcgcgatgag
     2521 gtcgacgtcc gaaccgcgat tgctgaagtc ggcgaacgaa tcgtcgttgt cgtaggtgcc
     2581 ccaggagaaa cagcgatggc cgccgatggc gccgggcttg ccgtcggtgt cggcgagggc
     2641 cgagacggtg atcacctcgt tgtaggccgc aggcacgcgg gccgcggcac tcgacgagtc
     2701 gttgccggcg gccgccacga ccgtgacgcc gctcgcgacg agccggcaga tcgccgcgtg
     2761 gaggatgtcc ttgttccagg cgccgcagtg gcggtcgtcg gagcccctct tcgcgacgct
     2821 catgttgacc gcctcgaatc gcggtcgggt cgggtcgacg ggatcgtgct gcgcggcgat
     2881 ccagtcgagg ccgcagacat accagctgag gaggccgtcg cccgtgtcgt cgaggatctt
     2941 gaccgcccac aaccggacgc cgggcgcgac gccgacgaca ccgatgccgt tgtcgatggc
     3001 cccgaccgtg cccgcgacat gggtcccgtg gccgtacacg tcgcgccacg ccgcgcgatt
     3061 cgaggtcgag cagttgtagc cgccggcgac gttgaggtcg gcgaccttcg cgatcccggt
     3121 gtcgacgatc gccacgtcgg catcgacccg cacatcgacg ccgtcgatct tcgcggcggt
     3181 cgagcggttg gccccgatcc gggtgatccc ggtcggcagc gcctgggact cggcctggat
     3241 cttctcgtcg gccacgatca tcgcgaccga tgcgtcatgg ctcagggcgg tgacctgggc
     3301 ccggtcgagg gtcgcggaat agccgcgcag gctgttgcga taggtccggt cggtcacgaa
     3361 gccgatccgc ttgccctgcc gttcgattgc ggcgtcgaca ttgctgcccg gcttgaggac
     3421 gacgatccag cggtcggtgg gatccgtggc ggcgcgcgcg gcgcgggtcg tcgccggatc
     3481 cttgcgggat gggccggtcg tctcgacgat cggggcggtc gccggctcgc cgaaatggct
     3541 cgggcgggtt gtcgcggcgg tggcgggatc cgtctcggaa cctggatccg ggatggccgc
     3601 cggcgaaggg tcagcggtgg gtggcggatc gacggacggc gtcggttcgc cgatcggagc
     3661 ggagctcgcg gaggggtccg gccccgcccc ggtggcactg cccacactgg acaggagcag
     3721 ggcggcggcg gcgagggcgg caaggcttcg gcgcatgacg ggtctccggc gaggatcggt
     3781 tcacgagccg acgcaccgga ctccttcgtg tccggacggc gcgacgacct gatcagcctc
     3841 ctccgaggac gctggtcgat gacaggaccc ggtggtccca tcgctggatg ggccgcacgt
     3901 cccaaccaca tcagacccta gtcctccacc ccgaacggag caactcgcca acggaccagc
     3961 gtcgatgccg acctgccgcc cgagggt
//
LOCUS       DATAIW010000464         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_136017, whole genome shotgun sequence.
ACCESSION   DATAIW010000464 DATAIW010000000
VERSION     DATAIW010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136017"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..2076
                     /locus_tag="VHM48_13860"
     CDS             19..2076
                     /locus_tag="VHM48_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2756548.1"
                     /translation="MRSLARPIGLGLAARGDVEEVVGWARQVRDQGLDSVWVHDSYFE
                     RDGVSFATAIALGIAADGDRDEDGFRVALGAANPFTRHPVVLAMTGSALDEILPGRFV
                     LGLGTGLPLRLKQMGIPYEPDAAVESVSVAIDQIRALWAGERLPSATPGLPPIQPMFP
                     PTHRIPIVIAAYRKAFVELAGRKADGYLARPAESIPSLRGILERLRASAIAAGRDPES
                     IESAGYLLSLVDKTRREALNRAKREPFVIYMMSVLSDVSLQRAGFDRELRDRIAAAWR
                     AEDYTTAGNLVPDELLDAFMLCGTREDVAAGAMAFHSTSGLQLPLLQPVLQEDRQIDE
                     LIAAAAIYASIPQPAGIADDGVPELAGGRRADRPATPSLADDRRLGAGDRLRRRAGAL
                     YEILRPFAFTASVIPVLAGAALAWVDEAWSWPPFIAAVLGAVFLHAGTNIVNEVYDVR
                     KGVDSITSPRASHAIVKGRMRERTALTIAATAFGLAILVGILLIALRGSAILVLGTLG
                     LIGGWGYTAPPLEYKNRGLGVPIVFLLMGPLMVEGAYFAVSGAWSSTALVLSIPVGLL
                     VAAILHGNEWRDIREDSRAGISTMSARIGRRWAHYGYVGLVLGAYIVLALSVIARILP
                     AWSMLAILSLPFLAQVIRSAELGATGQARAIAMIDLQTARLHLAFGSMLVLGLVLSRL
                     VGG"
     gene            2069..2767
                     /locus_tag="VHM48_13865"
     CDS             2069..2767
                     /locus_tag="VHM48_13865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2756549.1"
                     /translation="MAERPRRRPVAPGALEVGLVAAQAAFAATFRGPRRRFWQRMTVT
                     GLALGSLALLTSRPARQARIGPTDIAIGLASAATLYATFRVGDTFARRFVPGGDAQIR
                     DIYTLRTLRPRPEIAARLATIVGPAEELFWRGLVQEALMDRAGRWPGAALAAMAYGGV
                     HIVTGNFTLFGAAGIAGAHWCLLYAMGVPLGALIVSHVTWDVWIFLVQPTGDVAGLVP
                     AESATAGGSILPLA"
BASE COUNT          357 a         1099 c         1026 g          454 t
ORIGIN      
        1 cccatggagc ctccgtcgat gcgcagcctc gcccgtccca tcggtctcgg tcttgccgcc
       61 cgcggcgacg tcgaggaggt cgtcggctgg gcccgccagg tccgcgacca gggtctcgac
      121 tcggtctggg tccatgacag ctacttcgag cgggacgggg tcagcttcgc gaccgcgatc
      181 gcgctgggca tcgccgccga cggcgaccgg gacgaggacg gcttccgagt ggccctcggc
      241 gcggccaacc cgttcacccg ccatccggtc gtcctcgcga tgaccggctc ggcgctcgac
      301 gagatcctgc cgggccgctt cgtcctcggc ctcgggaccg ggctcccgct ccggctgaag
      361 cagatgggga tcccgtacga acccgacgcg gcggtcgaga gcgtctcggt ggcgatcgac
      421 cagatccgcg cgttgtgggc cggcgagcgc ctgccgtcgg cgacgcccgg gctgccgccc
      481 atccagccga tgttcccgcc gacccaccgg atcccgatcg tgatcgcggc ctatcgaaag
      541 gccttcgtcg agctggccgg ccggaaggcc gacggctatc tcgcccggcc ggccgagtcg
      601 attccgtcgc tgcgcggcat cctcgagcgg ctccgggcat cggcgatcgc cgccggccgg
      661 gaccccgagt cgatcgaatc ggccggctac ctcctgtccc tcgtcgacaa gacccggcgc
      721 gaggcgctca accgggccaa gcgcgagccg ttcgtcatct acatgatgtc ggtcctgtcc
      781 gacgtctcgc tccagcgcgc ggggttcgat cgcgagctcc gcgaccggat cgccgccgcg
      841 tggcgggccg aggactacac gaccgccggc aatctcgtcc ccgacgagct gctggatgcg
      901 ttcatgctgt gcgggacccg cgaggacgtc gccgcgggcg cgatggcctt ccacagcacg
      961 agcgggctcc agctgccgct gctccagccg gtgctccagg aagatcgcca gatcgacgag
     1021 ctgatcgcgg ccgccgccat ctacgcctcg atcccgcaac ccgcggggat cgccgacgat
     1081 ggggtcccgg aactcgccgg cggccgccgg gcggatcggc cggccacgcc gagcctcgcc
     1141 gacgatcgcc ggctcggcgc cggggatcgg ctgcgaaggc gggccggcgc cctgtacgag
     1201 atcctccggc cgttcgcctt caccgcgtcg gtgatcccgg tgctggccgg agccgccctc
     1261 gcctgggtcg acgaggcatg gagctggccg ccgttcatcg ccgcggtcct gggcgccgtg
     1321 ttcctccacg ccggaacgaa catcgtcaac gaggtctacg acgtccggaa gggcgtcgac
     1381 tcgatcacca gcccgcgggc gagccacgcg atcgtcaagg gccggatgcg cgagcggacg
     1441 gcgctgacga tcgcggcgac cgcgttcggc ctcgcgatcc tcgtcgggat cctgctcatc
     1501 gccctgcgcg gatcggcgat cctcgtcctc gggacgctcg ggctgatcgg cggctggggc
     1561 tacacggcgc caccgctcga gtacaagaac cgcgggctcg gcgtgccgat cgtgttcctg
     1621 ctgatgggtc cgctcatggt cgagggggcc tacttcgcgg tgagtggagc ctggtcgtcg
     1681 accgccctcg tcctgtcgat cccggtcggg ctgctcgtgg cggcgatcct ccacggcaac
     1741 gagtggcggg atatccgcga ggacagccgg gccgggatct cgacgatgtc ggcccggatc
     1801 ggccgacgct gggcgcacta cggctacgtc gggctcgtat tgggcgcgta catcgtgctg
     1861 gcactctcag tcatcgcgcg gatcctgccg gcgtggtcga tgctcgcgat cctgtcgctg
     1921 ccattcctcg cccaggtcat ccgctcggcc gagctcgggg cgaccggcca ggcacgggcg
     1981 atcgcgatga tcgacctcca gaccgcccgg ctccacctgg cgttcggctc gatgctcgtc
     2041 ctcgggctcg tcctctcgag actcgtcggt ggctgagcgg ccgcgtcgac ggccggtcgc
     2101 gccgggcgcc ctcgaggtcg ggctcgtcgc ggcccaggcg gcgttcgcgg cgacgttccg
     2161 cggcccgcgc cggcggttct ggcagcggat gaccgtgacc ggtctcgccc tcgggtcgct
     2221 ggccctcttg accagccggc cggcgcgcca ggcgcggatc ggcccgaccg atatcgcgat
     2281 cgggctggcg tcggcggcca cgctgtacgc cacgttccgc gtcggcgaca cgttcgcgcg
     2341 gcggttcgtg cccggcggcg acgcccagat ccgcgacatc tacaccctcc ggacgctccg
     2401 cccccggccc gagatcgcgg cccgcctcgc cacgatcgtc ggcccggccg aggagctctt
     2461 ctggcgcggg ctcgtccagg aggccctgat ggatcgcgcc ggccggtggc cgggagcggc
     2521 gctcgcggca atggcctacg gcggcgtcca catcgtgacc ggcaacttca cgctgttcgg
     2581 cgcggcgggg atcgccggcg cccactggtg cctgctctac gccatgggtg tcccgctcgg
     2641 cgccctcatc gtcagccacg tgacctggga cgtctggatc ttcctcgtcc agccgaccgg
     2701 ggatgtcgcg ggcctcgtcc cggccgagtc cgcgaccgcc ggaggctcga tcctgcctct
     2761 tgcctgacgg cgggaaacat gctagcgttc agtagcataa aactccgcgg ccaaggagcc
     2821 ccagccatgt ccacccttcc cgagccggtc gagcagctgc tcggcgcggc cctcgtcggc
     2881 gagctgaccg tcgtggacgc gaccggtcat ccggtcacgt acccgctgat cccgct
//
LOCUS       DATAIW010000465         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_136085, whole genome shotgun sequence.
ACCESSION   DATAIW010000465 DATAIW010000000
VERSION     DATAIW010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136085"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..374)
                     /locus_tag="VHM48_13870"
     CDS             complement(99..374)
                     /locus_tag="VHM48_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756550.1"
                     /translation="MAVVSVLAVAAVAIGVWVFANEGPGMAWFWSVAVVSIIALAVLY
                     PYATACRHGNEGAVAVWLALFLGPLSLIILAPWALALRSRYDREQGG"
     gene            583..768
                     /locus_tag="VHM48_13875"
     CDS             583..768
                     /locus_tag="VHM48_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756551.1"
                     /translation="MDGFLQAIGRGISGLVVGSFTVIGQTLHGMVDTLNRALPGGMLA
                     AVVFVVLVVAAWQLAKR"
     gene            complement(819..1076)
                     /locus_tag="VHM48_13880"
     CDS             complement(819..1076)
                     /locus_tag="VHM48_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756552.1"
                     /translation="MGFLRRLLGNGDRDGADDSEPAAAEPADAATVDAEERARELELA
                     RFDQERVDDLVRRQQRYADRSWTPPAQGGERRADDEDAAGT"
     gene            complement(1079..1864)
                     /locus_tag="VHM48_13885"
     CDS             complement(1079..1864)
                     /locus_tag="VHM48_13885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2756553.1"
                     /translation="MCFDLDSRPPIPEIAGGALDSTELTLEAADGNRFRAFRARATTP
                     SGAGIVILPDVRGLHPFYEELALRFAENGVDALAIDYFGRTAGVGERGDEFEFMPHVE
                     QVTWPNASVDVQAAARHLRMEDEGRVGALFTIGFCFGGRLAYLTATLGLELAGSIGFY
                     GIPAGPGRGGTAAPADVTDRMTNAILGVWGGADGATSPEAVGQFDEALTASGVEHRFV
                     TYDGAPHSFFDRKAEQFAHASTQAWDETLTFVRGHTASPVGGG"
BASE COUNT          292 a          759 c          737 g          307 t
ORIGIN      
        1 ctgcccggcc ggatcgcggt cgtctcgccc cgcaacctcg gcgcccacct ggtgtcgcgc
       61 gcctgacggg catcgagttc cggatccgtg ccgggggcct acccgccttg ctcccggtcg
      121 tatcgtgacc ggagagcgag cgcccaaggc gccaggatga tcagcgagag tggtccgagg
      181 aagagggcga gccagaccgc gacggcccct tcgttcccgt gtcggcaggc cgttgcgtag
      241 ggatacagaa ccgcgagcgc gatgatcgac acgaccgcca ccgaccagaa ccaggccatt
      301 ccggggcctt cgttcgcgaa cacccagacg ccgatcgcaa ccgcggcaac cgcaaggacg
      361 gagacgaccg ccatctcgcg agtcgcgcgc cgaccatcgg atcggccagc gtccatcgtc
      421 atcgcctcct gaacccgttc gccccgcgat cgtcgtcgtt ccgctgggcc aacattgtgc
      481 cgtctggcct gactggctcg cgtctccgac cggcgcggac cgcggtcggg ggaggtcttg
      541 cgcggtccgg ggcgattgac cgcacagcgc agactcgggt gcatggacgg cttcctgcag
      601 gcgatcgggc gcgggatcag cggcctcgtc gtcggctcgt tcacggtcat cggccagacg
      661 ctgcacggga tggtcgacac gctcaaccgg gcgctgccgg gcgggatgct cgcggccgtc
      721 gtcttcgtcg tcctggtcgt cgccgcctgg cagctcgcca agcgctgagc cctcgcctcg
      781 gccggcccgc cgcgccgcgc cgcgcggcca ccgctcactc aggttccggc ggcatcctcg
      841 tcgtcggctc gccgttcacc accctgggcc ggcggcgtcc acgatcgatc ggcgtagcgc
      901 tgctggcgcc ggacgaggtc gtcgacccgt tcctggtcga accgggccag ttcgagctcg
      961 cgggcccgtt cctcggcgtc gaccgttgcc gcgtcggcgg gctccgccgc cgccggctcg
     1021 gaatcgtcgg cgccatccct gtcgccattg ccgagcagcc gtcgcaggaa gcccatcctc
     1081 agccgccgcc gaccgggctc gccgtatgcc cgcggacgaa tgtcagcgtc tcgtcccagg
     1141 cctgcgtgct ggcgtgcgca aactgctcgg ccttgcgatc gaagaagctg tgcggagcgc
     1201 cgtcgtaggt cacgaaccgg tgctcgacac ccgacgcggt gagtgcctcg tcgaactgcc
     1261 cgacggcctc cggcgaggtc gcgccgtcgg caccgcccca gacgcccagg atcgcgttcg
     1321 tcatccggtc ggtcacgtct gccggagcgg ccgtcccgcc acggcccgga ccggcgggga
     1381 tcccgtagaa gccgatcgat ccggcgagct cgagcccgag ggtcgccgtc agataggcga
     1441 ggcgtccgcc gaagcagaag ccgatcgtga agagcgctcc gacccggccc tcgtcctcca
     1501 tccgcagatg cctcgccgcg gcctggacgt cgaccgaggc gttcggccat gtgacctgct
     1561 cgacatgggg catgaactcg aactcgtcgc cgcgctcgcc gacgccggcc gtccggccga
     1621 aatagtcgat cgcaagggcg tcgacgccgt tctcggcgaa ccgcagggcg agctcctcgt
     1681 agaaggggtg caacccgcgg acgtccggca ggatcacgat gccggccccg ctcggcgtcg
     1741 tcgcccgggc gcgaaaggcg cggaagcggt tgccgtcggc cgcctcgagg gtcagctcgg
     1801 tcgagtcgag ggcgccgccg gcgatctccg ggatcggcgg ccgggagtcg aggtcgaaac
     1861 acatgggcgg cgttcctcca gtgatcgggc gccaaggcta ccgcggagtc cgtccaagcg
     1921 cggtgcgcga taaccggcgg tgaagcgccg acgggcatcg tgcgcgtgat ggatcgctgg
     1981 cggttcggtc gggtcgtgcg cgcacttcgt caacggcatg gttggagaca ggaggatctc
     2041 gcaaagcacg ccggcgtctc gcgcagcgtc gtcggccgga tcgagcgcgg tgaac
//
LOCUS       DATAIW010000466         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_227728, whole genome shotgun sequence.
ACCESSION   DATAIW010000466 DATAIW010000000
VERSION     DATAIW010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227728"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VHM48_13890"
     CDS             <1..1419
                     /locus_tag="VHM48_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756554.1"
                     /translation="PIIGSEHLALLLRGPGPGGRDVGAELAEAFRVVRRELGVEAVRA
                     HAILDDSLAVYHDSGDGPTLDFSRVDEVLDRLLETGLRPIVELSFMPRDLASEPHATV
                     FAYAAIISPPRDLERWAALVGDLVAHLAERYGRGEVRRWAFEVWNEPNLCVFWSGAES
                     DYLQLYEASVRAVKSVDPSFRVGGPATAAAAWVDDLLEFCQATGVALDFISTHTYGLP
                     PLDLRPVVARFGRPDLPLWWTEWGVSPRLGAPINDSAWAAPLVARGMRSAAGRVEALA
                     YWVASDHFVEGGDAPTLFHGGFGLLTIGNLRKPRFWAIAMLERLGENELASELDGDGA
                     RSLVEAWASSDPDGRVAIAVWNGTLDQSKGAGVRALGRSVTLKVEGLRPGKFELRHHR
                     VDARHSNIVRTWKRLGRPDWPDDAGWTRLRAADRLEALEPPRPVRARQGRLELEFELP
                     MPAMSLVELVPIGHRNDRVAHP"
BASE COUNT          192 a          570 c          553 g          232 t
ORIGIN      
        1 ccgatcatcg gctccgagca cctcgccctc ctcctccgcg ggccggggcc tggcgggcgc
       61 gacgtcggcg cggagctggc cgaggccttt cgggtcgtcc gccgcgagct cggcgtcgag
      121 gccgtgcggg cgcatgccat cctcgacgac tcgctcgcgg tctaccacga ctcgggcgat
      181 gggccgaccc tcgatttctc aagggtggac gaggtcctgg atcggctgct cgagaccggc
      241 ctcaggccga tcgtcgagct ctcgttcatg ccgcgcgacc tcgcgagcga gccccacgcg
      301 acggtgttcg cctacgcggc gatcatctcg ccgccgcgcg acctggagcg gtgggcggcc
      361 ctcgtcggcg acctcgtcgc ccacctggcg gagcggtacg gtcgcggcga ggtgcgccgc
      421 tgggcgttcg aggtctggaa cgagccgaac ctctgcgtct tctggtcggg cgccgagtcc
      481 gactatctcc agctgtacga ggcaagcgtc cgggccgtca aatcggtcga cccgagcttc
      541 cgggtcggtg gcccggccac cgcagccgcg gcctgggtcg acgacctcct ggagttctgc
      601 caggcgacag gcgtggcgct cgacttcatc tcgacccaca cctacggcct gccgccgctc
      661 gacctccgtc cggtcgtcgc tcgtttcggg cggcccgacc tgcccctgtg gtggaccgaa
      721 tggggcgtca gcccgcgcct tggcgcgccg atcaacgaca gcgcgtgggc cgcgccgctc
      781 gtcgcgcgcg gcatgcgatc ggcggccggc cgcgtcgaag cgctcgccta ctgggtcgcg
      841 tcggaccact tcgtggaagg cggcgacgcg ccgacgctgt tccatggcgg gttcgggctg
      901 ctcacgatcg gcaacctccg caagccccgc ttctgggcga tcgcgatgct ggaacggctg
      961 ggcgagaacg agctggcgag cgagctggac ggcgacggcg cgcgttcgct cgtcgaggcg
     1021 tgggcgtcga gcgatcccga cggccgggtc gcgatcgcgg tctggaatgg gacgctcgac
     1081 cagtcgaagg gggccggggt ccgcgccctc ggtcggtcgg tcacgctcaa ggtcgagggg
     1141 ctgcggccgg ggaagttcga gctgcggcac caccgggtcg acgcccgcca ctcgaacatc
     1201 gtccggacct ggaagcgtct cggccggcct gactggccgg atgacgccgg ctggacacgt
     1261 ctccgcgcag ccgaccgcct cgaggccctc gagccgcccc gcccggtccg cgcccgccaa
     1321 ggccggctcg agctggagtt cgagctgccg atgccggcca tgtcgctcgt cgagctcgtg
     1381 ccgattggcc accggaacga ccgtgtcgcc catccgtgac cggcggcagg tccgcgaccc
     1441 gcggccgctg atcgcaaccg agctgcgaaa ccgggccggc ctctcgatcg agctcctgga
     1501 caacggctcg ctcttcgcga tccgccacgg cgacgtcctg atcaacc
//
LOCUS       DATAIW010000467         6258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_197961, whole genome shotgun sequence.
ACCESSION   DATAIW010000467 DATAIW010000000
VERSION     DATAIW010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197961"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..252)
                     /locus_tag="VHM48_13895"
     CDS             complement(121..252)
                     /locus_tag="VHM48_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756555.1"
                     /translation="MTRTTSRPVNYRITRRMAAVHRAHATAPHLGCVLCLVGRAPGR"
     gene            729..1019
                     /locus_tag="VHM48_13900"
     CDS             729..1019
                     /locus_tag="VHM48_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756556.1"
                     /translation="MAPIALQVSVDGRHVLVKVSWYGGVEPCSVLDSVRVERSGTEIA
                     LTPIEGSSDRTAMCVEMAVLKATIVDLGELDPGTYRISAPGSDATPVVITVD"
     gene            1079..2257
                     /locus_tag="VHM48_13905"
     CDS             1079..2257
                     /locus_tag="VHM48_13905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX2756557.1"
                     /translation="MNLFDVAVLVLLVGAVLIGVKSGALPQLGGLIGAFGGGAVAVLG
                     LPWIEGPLGAVPPEIRAILVLAGILFLVGIGEAVGAALGRTLAIRLRGRVLDRFDRIL
                     GGFLGAAQALLVVWLIGGLLAAGPMRNLAVQAQTSLVVRGLTAILPAPTEIAAELGQL
                     LHDTGIPDLFVGLDPLPAPDVDRPTDPAAQAIASAAIPSLVRVSAATCQFQSSGTGFA
                     VARGYVVTNAHVVAGASLIRIQAPNGRPLDAVTVFDDPELDVALLWVRDLDVAPLRFA
                     ATDPDRGAVGATLGFPHGGGLLIEPAAVAGAYDAQGRDIYGTRRVSRRILELRAVVDQ
                     GDSGGPFILTDGSVGGVVFAEARTDENVGYALTPTSVAAAIRPSIGRTGPVDTGACIH
                     "
     gene            2406..4121
                     /locus_tag="VHM48_13910"
     CDS             2406..4121
                     /locus_tag="VHM48_13910"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2756558.1"
                     /translation="MTDPITPSTDPDTRLASLAAEAWDSQMAAHPVLATALGDRRFDD
                     RLRKNGPGAGAADAARLSGLSHRAAAIDPATLSADDQVTHSALVDFLGFELDLAEAGL
                     AAWSVDPLDGPQVAFFNVPSFQPVRTLADGEALVARWRAIGPWIDRLTGTTRDALARG
                     IAAPRALVRNVVGELDDLLARPVDDWPLVDPSREDHPGWTPDQRRAQADAIRDAVADG
                     IRPAFARYRAFLADELGPVARGDERPGLSHVPGGLEAYARLVRSHTTLDLAPEEIHRI
                     GLEETERIDAEFRELGGRLLRTNDEATILARLRADPALHFSTPAEVFDVAEASLARAN
                     AAIPAWFGRLPKTPCVVVEMGAHEAKYSTIAYYREPASDGSRPGSYYINTSAPATRPR
                     YEAETLAFHEAVPGHHLQIAIAQELDGLPAFRRLGGPTAYVEGWGLYSERLSAEMGLL
                     SGDMDRFGIASFDAWRACRLVVDTGLHALGWSRDRAIGFMVEHTALAENNIANEVDRY
                     LAIPGQALAYKLGQREILRLRDEARTALGADFDIRAFHDVVLGQGAVGLATLGSVVEA
                     WVRAG"
     gene            complement(4137..4589)
                     /locus_tag="VHM48_13915"
     CDS             complement(4137..4589)
                     /locus_tag="VHM48_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2756559.1"
                     /translation="MAATLERVMATHTDQKLELLKRTPLLAGLGRKEIEEVGRLAEEV
                     DVAAGRVLMREGDTGREFYVVVSGTVGIDRGGTRIRTMEPGDFFGEIALLDEGPRTAS
                     ATTDTAATLLVLGHREFHSLMDQFPAIRTCVLEALAKRIRNQQPEAAN"
     gene            4879..>6258
                     /locus_tag="VHM48_13920"
     CDS             4879..>6258
                     /locus_tag="VHM48_13920"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2756560.1"
                     /translation="MPDLPHPYDRPSDPAKRHSAALTDGPDRAAARGMLKAIGFTDED
                     LAKPIVGVGTSWIETMPCNYNHRRLAESVKAGIRAAGGTPMEFGTISVSDGVSMGTEG
                     MKASLISREVVADSIELVARGHLFDGLVCIFGCDKTGPGAAMALGRLDIPGLTLYSGT
                     IHPGIRSAVMGGNGSRDATVVTIFEAIGAYRAGKISLDELYEVESASCPGPGACGGQF
                     TANTMSMVMETLGLSPAGLNGIPAEDPAKDEAARRAGEMVMRLVRDDIRPSRFVTPTS
                     IDNAIASVAATGGSTNAVLHLLAIAHEYGVPLEIDRFGTIADRTPIVADLQPGGRYTA
                     THLYDAGGLALVFRELLGAGRIDGAAPNVDGRTTAEVAAAATATEGQDVVVPFDRPIK
                     STGGLAILRGSLAPDGCVVKLAGHERRLHRGPARVFDSEGECYAAVRDRRIVAGDVVV
                     IRYEGPVGGP"
BASE COUNT          812 a         2307 c         2212 g          927 t
ORIGIN      
        1 ggcctgggag gccggccacc ccgcccggat cgacacgcgt gccggggccg cctgagcgcc
       61 ggaccggccc tcgagccgcc cgcccggcgg agggcgggcg cggtcgtcgg gcggaggaga
      121 tcagcggccg ggggcgcgcc cgaccaggca gaggacgcag ccgaggtgtg gggccgtcgc
      181 gtgggcgcgg tggaccgcgg ccatccggcg ggtgatccgg tagttgacgg ggcgggaggt
      241 cgtgcgggtc atcgggagcg gtcctttcga tcgatggtcg ctcctctcgg aacgagaccc
      301 gatcctgccg acccaccgca gcggccggca gggccgcgcg acctcgatcg gcatggacag
      361 gtgacgatgc cgggcgtgcc cggccgggga acccgcggcc gatcgatgtc gtctaccgag
      421 ccatgacgac ctccaccgcc gcgcttcgag cggttctcgc catcgccatc gcaaccgcca
      481 tcggcctgtc cgcgtgttcg ggcgcggcgg cgccggcgac aacgccgtcg ggcgacgcca
      541 cggcatcccc ggtgccagcc tcggcaccgt ctgacggagc cgatccgggc gcgggcggcg
      601 gcggcggctc agctgggaat ccgggcacgg gaatcgggat cgatcccgtg gaccccgcac
      661 ccgtcgatcc gggaggcggg cagccgatgc tcgtccgacc ggtcccagga cgggccgatc
      721 cgcatccggt ggccccgatc gccctgcagg tgtcggtcga tggacggcac gtcctcgtca
      781 aggtcagctg gtacggcggc gtcgagccct gctcggtcct cgattcggtc agggtcgagc
      841 ggtcggggac ggagatcgcc ctcacgccga tcgaaggctc gagcgatcgg accgcgatgt
      901 gcgttgagat ggcggtcctc aaggcgacga tcgtcgacct cggcgagctc gatcccggaa
      961 cctaccggat ctcggcgccg ggcagcgacg ccacgccggt cgtgatcacg gtcgactgat
     1021 ccgggcgcgc cggcccattc cggacgcggg gaccgcagcc aacccatact tcgcggcggt
     1081 gaacctcttc gacgtcgccg ttctcgtcct cctcgtcggc gcggtcctga tcggggtcaa
     1141 gtccggagcc ctgccgcagc tcggaggcct gatcggggcg ttcggcggcg gggccgtcgc
     1201 cgtcctcggc ctgccctgga tcgagggccc tctcggtgcc gtcccgccgg agatccgggc
     1261 gatcctggtc ctggccggga tcctgttcct cgtcgggatc ggggaggcgg tcggcgcggc
     1321 cctcggacga acccttgcca tccgcctgcg cggcagagtc ctcgatcgct tcgaccggat
     1381 cctgggcggc ttcctcggcg ccgcccaggc gctgctggtg gtctggctga tcggcggcct
     1441 gctcgccgcc ggcccgatgc gcaatctcgc ggtccaggcg cagacatcgc tcgtcgtgcg
     1501 cggcctgacc gcgattcttc cggccccgac agagatcgcc gccgagctcg gccaactgct
     1561 ccacgacacc ggcatcccgg acctcttcgt gggcctcgat ccgcttcccg ccccggacgt
     1621 ggatcggccg acggatccgg ccgcccaggc gatcgcctcc gctgcgatcc cgagcctggt
     1681 ccgggtgtcg gccgcgacct gccagttcca gtcatccgga accggcttcg cggtggcccg
     1741 cgggtacgtc gtcacgaacg ctcatgtggt ggccggcgcg tccctgatcc ggatccaggc
     1801 acccaacgga cgaccgctcg acgcggtcac cgtgttcgac gatccggagc tcgatgtcgc
     1861 cctcctgtgg gttcgtgacc tcgacgtcgc tcccctccgg ttcgccgcca cggacccgga
     1921 ccgcggcgcg gtcggcgcca cgctcggctt cccgcacggt ggcggcctgc tgatcgaacc
     1981 ggccgcggtc gcgggggcct acgacgccca gggtcgcgac atctacggaa cgcggcgggt
     2041 cagccgccgg atcctcgagc tgcgggcggt cgtcgaccag ggcgacagcg gcggcccctt
     2101 catcctcact gacggatccg tcggtggggt cgtgttcgcc gaagcgcgga cggatgagaa
     2161 tgtcggctac gccttgacgc cgacgtcggt cgcggctgcg atccggccgt ccatcgggcg
     2221 aaccgggccg gtcgacaccg gcgcctgcat ccattgacgg gcgccccgag ccgggcgcgc
     2281 cagcccgtga gtcgctgatc gggggcgcgg ccgtcgcggc cgtcgcggcc gtcgcggtcg
     2341 tcccggtcgt cccgggccgg aaggtgggcg cgacgaaacg gatcacggac ctctaggatc
     2401 ggcggatgac cgatccgatc accccttcga ccgacccaga cacccgtctt gcctcgctcg
     2461 cggccgaggc ctgggacagc cagatggccg cccatccggt cctcgcgacg gccctcggcg
     2521 accgccgctt cgacgaccgt ctccggaaga acggacccgg ggcgggtgcc gccgacgcgg
     2581 cgcggctcag cggcctgtcg caccgggcgg ccgccatcga tccggcgacg ctctccgccg
     2641 acgatcaggt cacccactcg gcgctcgtcg acttcctcgg gttcgagctc gatctcgccg
     2701 aagccgggct cgccgcctgg tcggtcgatc cgctggacgg gccgcaggtc gcgttcttca
     2761 acgtgccgtc gttccagccg gtccggaccc tggccgacgg cgaggctctc gtggcccgct
     2821 ggcgggcgat cgggccgtgg atcgatcgcc tgaccggcac cacccgcgat gccctcgccc
     2881 gcggcatcgc ggcgccacgg gcgctcgtcc gcaacgtcgt cggcgagctc gacgacctgc
     2941 tggcgagacc ggtcgacgac tggccgctcg tggatccgtc ccgagaggac catccggggt
     3001 ggacgccgga ccaacgtcga gcccaggccg acgcgatccg ggacgcggtc gccgatggca
     3061 tccgacccgc cttcgcccgc taccgggcgt tcctggcgga cgagctcggg ccggtcgctc
     3121 gcggcgacga gcgccccggg ctgtcgcacg tcccgggcgg cctcgaggcc tacgcccgcc
     3181 tcgtccgctc ccacaccacg ctcgacctcg cgcccgagga gatccaccgg atcggcctcg
     3241 aggagaccga gcggatcgac gccgaattcc gcgagctcgg cgggcggctc ctgcggacga
     3301 acgacgaggc gacgatcctc gcccggctgc gggccgatcc ggccctccat ttctccacgc
     3361 cggccgaggt gttcgacgtc gccgaggcgt cgctcgcccg ggccaacgcg gcgatcccgg
     3421 cctggttcgg ccgcctgccg aagacaccgt gcgtcgtggt ggagatgggc gcccacgagg
     3481 cgaaatactc gacgatcgcc tactaccgcg agccagcctc ggacgggagc cggcccggca
     3541 gctactacat caacacgtct gctccggcga cgcggccgcg ctacgaggcc gagaccctgg
     3601 ctttccacga ggccgtcccg ggtcaccatc tccagatcgc gatcgcccag gagctcgacg
     3661 ggctgcccgc cttccggcgg ctcggcgggc cgacggccta tgtcgagggc tggggtctct
     3721 acagcgagcg cctgtcggcc gagatgggcc tcctgtcggg tgatatggat cggttcggca
     3781 tcgcgtcctt cgacgcgtgg cgggcgtgcc ggctcgtcgt cgacacgggc ctccacgccc
     3841 tcggctggag ccgcgatcgg gcgatcggat tcatggtcga gcacaccgcg ctcgccgaga
     3901 acaacatcgc caacgaggtc gaccggtatc tcgccattcc cggccaggcc ctcgcctaca
     3961 agctcggcca gcgcgagatc ctgcgacttc gcgatgaggc ccgcaccgcg ctcggtgccg
     4021 acttcgacat ccgggcgttc cacgacgttg tcctcggcca gggggccgtc ggcctcgcga
     4081 cgctggggtc ggtcgtggaa gcctgggtcc gggcgggctg agcgcgagcg cagccctcag
     4141 ttcgcggctt cgggctgctg gttgcggatc cgcttggcga gagcctcgag gacacacgtc
     4201 cggatcgccg gaaactggtc catcagggag tggaactcac gatggccgag gacgaggagc
     4261 gttgcggccg tgtcggtcgt cgcgctggcg gttcgcggac cttcgtccag cagggcgatc
     4321 tcgccgaaga agtcaccggg ttccattgtc cggatccgcg ttccgccgcg atcgatcccg
     4381 acggtcccgc tgacgacgac gtagaactcg cgtcccgtgt cgccctcgcg catgaggacc
     4441 cggccggcgg ccacgtcgac ctcctccgcg agacgcccga cttcctcgat ctccttccgg
     4501 ccgagacccg ccaggagcgg cgtccgcttg agcagttcca gcttctggtc ggtatgcgtc
     4561 gccatcaccc tctccaacgt cgcggccatg cgtccgggca gcataacgga ggaagtgcca
     4621 cgttcaatgg ctgtcgcgca atggctgtcg cgccttcgcg gccgatcgcg gcgttgaccc
     4681 ctgcggtcct cggggtcgcc ttgcgctcga ccacgcatgc gacgaacgca gtcgccggtt
     4741 ggcgtgccct tcgcggccga tcgcggcgtt gcgccctgcg ggcggtcgtg tcttcgctgg
     4801 gcgccggtcc gggaccgacc acgccctaca ctggacgctc gcccgtccgc gctcgtccat
     4861 cgcaccggag accgctcgat gcccgacctg ccgcacccct acgatcgtcc gtccgatccc
     4921 gcgaaacggc attcggccgc gctcaccgac ggccccgatc gggccgcggc gcgcggcatg
     4981 ctcaaggcga tcggcttcac cgacgaggat ctcgccaagc cgatcgtggg cgtcgggacg
     5041 agctggatcg agacgatgcc ctgcaactac aaccatcggc gactcgccga gtcggtcaag
     5101 gcgggcatcc gggcggccgg tgggacgccg atggagttcg ggacgatctc cgtcagcgat
     5161 ggcgtgtcga tgggcaccga gggcatgaag gcctcgctga tcagtcgtga ggtcgtcgcc
     5221 gattcgatcg agctcgtcgc ccgcggccat ctcttcgacg gcctcgtgtg catcttcggt
     5281 tgcgacaaga ccggcccggg cgcggcgatg gcgctcggcc ggctcgacat cccgggcctg
     5341 accctgtact ccggcacgat ccatccgggg atccggtcgg ccgtgatggg cggcaacggc
     5401 tcgcgcgatg cgaccgtcgt cacgatcttc gaggcgatcg gcgcctaccg ggccggcaag
     5461 atcagcctcg acgagctgta cgaggtcgag agcgcctcgt gtccggggcc gggcgcatgc
     5521 ggcggccagt tcacggccaa cacgatgagc atggtcatgg agacgctcgg cctgtcgccg
     5581 gccgggctga acgggatccc ggctgaggat ccggccaagg acgaggccgc ccgtcgggcc
     5641 ggcgagatgg tcatgcgcct cgtccgcgac gacatccggc cgtcgaggtt cgtcaccccg
     5701 acctcgatcg acaacgcgat cgcctcggtc gccgcgacgg gcggctcgac gaacgcggtc
     5761 ctccatctcc tcgcgatcgc ccatgaatac ggcgtcccgc tcgagatcga ccggttcggc
     5821 acgatcgccg accggacgcc gatcgtggcc gacctccagc ccggcggccg ctacaccgcg
     5881 acccacctct acgacgcggg cggcctcgcc ctggtgttcc gcgagctcct cggcgccgga
     5941 aggatcgacg gcgcggcccc gaacgtcgac gggcggacga ccgcggaggt cgcggccgcc
     6001 gcgaccgcga ccgagggtca ggacgtcgtg gtgccgttcg atcggccgat caagtcgacc
     6061 ggtggcctgg cgatcctgcg cggttccctc gccccggacg gctgtgtcgt caagctcgcg
     6121 ggtcacgagc ggcgactcca ccgcggtcct gcccgggtgt tcgactccga gggcgagtgc
     6181 tacgcggcgg ttcgcgatcg ccggatcgtc gccggtgacg tcgtcgtcat ccgctacgag
     6241 ggtcccgtcg gtgggccc
//
LOCUS       DATAIW010000468         9702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_227875, whole genome shotgun sequence.
ACCESSION   DATAIW010000468 DATAIW010000000
VERSION     DATAIW010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227875"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VHM48_13925"
     CDS             complement(<1..755)
                     /locus_tag="VHM48_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2756561.1"
                     /translation="MVPYGGKSSRPPPTLRVVTWNIRAAIGPGEPFPPAWWRHVSPER
                     LERIAAILTELDADVATLQEVTIMTPNGAVHDQPAQLARLTDRSVRYAAAHAYPLIEP
                     ETGRTIGSAMWGNAILTRDSIADGFALGLPRAANDDLVEPIEADHPLAGVRYGDTDPG
                     HREPRCLVGGRAAGITIATTHLSYVGRMQRRRQVQAGRAAADALPGPLVLTGDFNASI
                     AADELAPLRDVLVDAFAAVGVAPTDPARASCGS"
     gene            802..3450
                     /locus_tag="VHM48_13930"
     CDS             802..3450
                     /locus_tag="VHM48_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX2756562.1"
                     /translation="MSDARQPTDRDLPGWDRDQEALARARAIRDRMTLEEKVEFVTGD
                     RGDWNYAFYNGPIDHLGIPALTMADGPAGVRINNQSVHDGRSTALPAPIALAATWDPE
                     QAAHYGDVIGAEAFASGHNVQLAPAVDIARAPLGGRTFEAFGEDPLLQSRMVVPEILA
                     IQRHPVHATIKHVVANNQEYQRFTVDARVDERTLREIYLPPFEAAVRDAHVAAAMGAF
                     NKVNGIFSCENRAILVDILKDEWGFRGWVMSDYDATWSTTAAANGGLDNEQPTGRFWG
                     PRLLAAIHAGEVEVAQLDDMVERILFPMAALGLLDRPVDIAPLPVEANGLVARGIAER
                     SMVLLQNDGILPLAADGVRSIAVIGPDADNTSAAGAGSGMLKAAYGVSALDGIRQRAG
                     DGVRVEYEAGVDPVSAAVLLPGLPAVPSDFLRPADDGNGEGRGLTGTYWPNLRFADMP
                     VLQRVDPEVALNLGFYNIPLFNGISPKLALTPGSLNGRMSARWTGSLVVPLTGRYELS
                     LTSFGAGRLFVDGELVIDIPGGGADDVPDAGATDNPPIPPMVPGVGPVERTTALDLLA
                     DRPATLRLEYAADSPELSPQAGPQFRLGWRPPDGLVQPGIAAAAELAARSDVAIVVAR
                     DYETEMMDRPDLLLPNNQDALIRAVAAANPRTVVVLMNGAPIETAAWDQDVAALVEAW
                     FAGQEQGHALASVLFGDVDASGRLPLTFPRSLAETPVSSAAQYPGVDGVATYSEGVMV
                     GYRGYDELGLQPRFPFGHGLSYTTFEYGGLEVDPAPAAEDDVVATVSFEVANTGSRPG
                     VEVAQVYVGRLPGPTRTPLRQLAGWARIGLDPGERRRVSVAIARRSVSVWDVDTHAWQ
                     TPAGDVSVEVGRSSRDLRLSARVGVR"
     gene            complement(3518..4414)
                     /locus_tag="VHM48_13935"
     CDS             complement(3518..4414)
                     /locus_tag="VHM48_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2756563.1"
                     /translation="MTQDALTGVGMAGAKPRTLSGRIIKMLNRTPIHIALGIVAIVWL
                     VPTIGLLITSFRPRNDIASTGWWETLGTLRFTLDNYVEVLNGQGILKAFINSLIITLP
                     STFLPIFLASLAAYAFSWLRFPFRDTLFLITIALLMVPIQVAFIPVLQLMKPFGLTQG
                     FAGLWLAHTAFALPFGIFLLRNFFITLPKDLIEAARIDGASSFGIFRRIVVPLSVPAI
                     AAYGIFQFLWVWNDLLVALIFAQGQGTKPITVQVLTLLGTYGSQWHILAASAFLLMFL
                     PLLVFFSLQRYFVQGLLAGSVK"
     gene            complement(4411..5370)
                     /locus_tag="VHM48_13940"
     CDS             complement(4411..5370)
                     /locus_tag="VHM48_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2756564.1"
                     /translation="MAVAPQAVPPAVPVKAAKKGGAREREPIAVLFLAPAMLLMAIFL
                     VFPAIYTIALSFFRGRANSQFSEFVGFTNYINLLTNDPNFINFKTFPPSGAIWNNVLW
                     LIFYTGIVLALGLIIAVMATRVRYEKQIKAIVFIPQAIAATALGVIWTFVYSANPDQG
                     FLNAFLGVFGIKPIAFLGDTNFVNAALIFVGVWAGVGFATVILSAAIKSISTEILEAA
                     RSDGANEFQVFLKIILPMVSLPMSVLAVTLIVGVIKLFDLIYVMTQGGPGAASRVIAF
                     TMYQEAFPGNQFGKGAAVAVVMLVILIPIMIFNIRRFRTESVT"
     gene            complement(5440..6804)
                     /locus_tag="VHM48_13945"
     CDS             complement(5440..6804)
                     /locus_tag="VHM48_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756565.1"
                     /translation="MAKFPRFLTLPIAAALIVSACGGGGTPAPSTGGGASNPPASTAP
                     TSGSSAATGSQAATGGSITVTSLWGGAEETAFQTVLADFKAKTGITANYVAQRTDYAT
                     VLRTAIAGGNPPDVAIMPGIGFLRSFAKDGSIKDFASLGIDVSALASNYAPGILEIGQ
                     VDGKQYGLMVKFNSKSTVFYRPDLFKTAGVTAEPKTWDEFKTVISTLKSKNIKPLGLG
                     AKDSWTLTDWFESIYIRQAGPDAYTKLFSKDGDWTDPTVKTAMETMKQVLIEDNVVGG
                     INGALGAAFTDGIGQVFKPAPDAAMYYEGGFVYGIATGDTNKDLKWGDTIGWFDFPGF
                     GNPGGVTIGGDVIAALTAKPGVKEFLQYMTTVDAGTTWAKTGIIISPIKGVSADIYPN
                     ESAKKEAAQVANAAAVRFDGQDLLPAGGPDFGAMLQSVIKGDPIDGLVSTFNTAQQAA
                     WAAE"
     gene            complement(7130..>9702)
                     /locus_tag="VHM48_13950"
     CDS             complement(7130..>9702)
                     /locus_tag="VHM48_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756566.1"
                     /translation="LAIGPWQILMDEFLVSGETIVRNLEIGWHAAEAFGAAMPVGYLP
                     DMFGHVAQMPQILRRAGIRHAVVWRGVPAAIDRHAFTWRSPDGSAVRAEYLVGGYGNG
                     AYLLAIPDRLADKVARYVEASRAFYGDRSILAMYGTDHAVPSPRLARIVADANAQRDD
                     ITVRIETLSTYIGAFDAALADPAAPDPAPTPVWIGELRSAARANMLMNVTSARIDIKA
                     AAGRAERMLERYAEPFAALHGGAWPERLLELAWRRLVDNSAHDSICGCSQDAVVAQVL
                     TRFAEAEQIGRGVLATALRPLAARAPRGTTVVVNPSPHDRSDVVELDLAVPAAWPAVE
                     LELGDGRRVPTQLVERPETVLRRLRMTGRQLPELFARRLHGRELFGSSLDGHEIVRDG
                     SAPELILRMDDPAAPVDFDVDTILDAVAAESGLDPDAIWDVAVVRGDRRRLLAAVPAP
                     PLGGALVRPVDAGQAAVPSNAAVMVEDRAIRNGLVSVQVADDGTLTIDGGGVRLTGAG
                     RLVDGGDFGDTYNYGPPATDRVVDRPRIVVTAAQERGPIRGSIEVVASYDWPVGLATD
                     GSSRSDATAPVEVTTTVELRAGEPFVRVVVAFTNPARDHRVRWHLPLPAATDHSSAEG
                     QFAVVDRGLTEEAGHGEVPTPTFPAHGFVHAAGATVLLDHVTEYELVDGRELALTVLR
                     STGLISRNDNPFREDPAGPEVAVPAAQIIGPWRFTFAVLPHAGSWSDADVLRAAEAYQ
                     LPFVTAVGRGGPGSADPEPDAPAAGVRIDGQGVVLSALRRRPAASGDGDELELRVVAE
                     IDTATVASISGRRLAAAQHIDLLGRLGADLVLEPDGSLRLALGPWEIRTVRIRFAPPA
                     "
BASE COUNT         1501 a         3266 c         3484 g         1451 t
ORIGIN      
        1 catgagccgc acgacgcccg agccgggtcg gtgggcgcga ccccgaccgc ggcgaaggcg
       61 tcgacgagca cgtcacggag cggggccagc tcatccgcgg cgatcgacgc gttgaagtcg
      121 ccggtcagga cgagcggacc gggaagcgcg tcggcggcgg cccgaccggc ctggacctgg
      181 cgccggcgct gcatgcgccc gacgtagctg agatgggtgg tcgcgatcgt gatcccggcc
      241 gccctcccgc cgaccagaca gcgcggttcg cggtgaccgg ggtccgtgtc tccgtagcgg
      301 acgccggcca gcgggtgatc ggcctcgatc ggctcgacga ggtcgtcgtt cgcggcgcgc
      361 gggaggccga gcgcgaaccc gtccgcgatc gaatcccggg tcaggatcgc gttgccccac
      421 atcgccgacc cgatcgtgcg cccggtctcg ggctcgatca ggggatacgc gtgggcggcc
      481 gcgtagcgga cggatcggtc tgtcagtcgg gcgagctggg ccggctgatc atggacggct
      541 ccgttcgggg tcatgatcgt gacctcctgg agcgtggcga cgtccgcgtc gagctcggtc
      601 aggatcgccg cgatccgctc gagccgctcg ggcgatacgt gccgccacca ggccggcggg
      661 aagggctcgc cgggaccgat tgccgcccgg atgttccagg tgacgacccg gagggtgggc
      721 ggcggtctgg acgatttccc cccgtacggc accatcgcgt cacaataagg gggttgtgcg
      781 aagaccatcg ggagagggtt gatgtcggac gctcgccagc cgacggaccg cgatctgccg
      841 ggctgggatc gtgatcagga agccttggct cgggcacggg cgatccggga ccggatgacg
      901 ctcgaggaga aggtcgagtt cgtcaccggc gaccgtggcg actggaacta cgcgttctac
      961 aacggcccga tcgatcacct cgggattccg gccctgacga tggccgacgg ccccgccggt
     1021 gtccgcatca acaaccagtc cgtgcacgac gggcggagca ccgcgctgcc ggccccgatc
     1081 gccctcgcag ccacctggga tcccgaacag gcggcgcact acggcgacgt catcggcgcg
     1141 gaggcgttcg caagcggcca caacgttcag ttggctccgg ccgtggacat cgcccgggcg
     1201 ccgctcggcg ggcggacgtt cgaggcgttc ggggaggacc cgctgctcca gtcgcggatg
     1261 gtcgtccccg agatccttgc catccagcgg catcccgtcc acgcgacgat caagcacgtc
     1321 gtcgcaaaca atcaggagta ccagcgcttc acggtcgacg cccgggtgga cgagcggacg
     1381 ctccgcgaga tctacttgcc gccgttcgag gcggcggtcc gcgacgcgca cgtggctgcc
     1441 gccatgggcg ccttcaacaa ggtcaacggc atcttctcgt gcgagaaccg ggccatcctt
     1501 gtcgacattc tcaaggacga gtggggcttc cgcggctggg tcatgagcga ctatgacgcg
     1561 acgtggagca cgaccgccgc ggccaacggc ggtctcgaca acgagcagcc gaccggccgc
     1621 ttctggggtc cgcgtctcct cgccgcgatc cacgcgggcg aggtcgaggt cgcgcagctc
     1681 gacgacatgg tcgaacggat cctgttcccg atggccgccc tgggtttgct cgatcgcccc
     1741 gtggacatcg caccgctgcc ggtcgaggcg aacgggctcg tcgcacgcgg gatcgccgag
     1801 cggtccatgg tcctgctcca gaacgatggc attctgccgc tcgcggccga cggcgtgcgg
     1861 tcgatcgcgg tcatcggccc ggacgccgac aacacgtcgg ccgccggcgc cggcagcggc
     1921 atgctcaagg ccgcatatgg ggtgagcgcc ctcgacggca tccggcagcg cgccggcgac
     1981 ggggtccggg tcgagtacga ggcaggagtt gatcccgtgt cggcggcagt gctgttgccg
     2041 ggcttgccgg cggtgccgtc ggacttcctg cgcccggctg acgacggcaa cggcgagggc
     2101 cgcggcctga ccggcacgta ttggccgaat ctccgattcg cggacatgcc ggtcctccag
     2161 cgcgtcgacc cggaggtcgc gctcaacctc ggcttttaca acatcccgct cttcaacggc
     2221 atctcgccga agctggcgct gacgccgggc tcgctcaacg ggcggatgtc cgcccggtgg
     2281 acgggcagcc tcgtcgtccc gctgaccggc aggtacgagc tgtccctgac gagcttcggc
     2341 gccggccggc tgtttgtcga cggcgagctc gtgatcgata tcccgggcgg cggcgccgat
     2401 gacgtgcccg acgcgggcgc gaccgacaat ccgccgatcc cgccgatggt tcccggcgtg
     2461 ggaccggtgg agcggacgac ggcgctcgat ctcctggccg atcgtcccgc gaccctgcgg
     2521 cttgagtacg ctgccgattc cccggagctc agtccccagg ccggaccaca gttccggctc
     2581 ggctggcgcc cgcccgacgg cctcgttcag cccgggatcg ccgccgctgc cgagctcgcg
     2641 gcgcgctcgg atgtggcgat cgtcgtcgcc cgcgattacg agacggagat gatggaccgc
     2701 ccggaccttc tcctcccgaa caaccaggac gccctgatcc gggccgtcgc cgcagcgaac
     2761 ccgcggacgg tcgtcgtgct gatgaacggg gcgccgatcg agaccgcggc ctgggatcag
     2821 gacgtcgccg ccctcgtcga ggcgtggttc gccggccagg agcagggcca cgccctcgcc
     2881 agcgtcctgt tcggcgacgt tgacgcgtcc ggccgtcttc cgctgacgtt cccgcggagc
     2941 ttggccgaga cgccggtctc gagtgcggcg cagtatccgg gagtcgatgg ggtcgcgacc
     3001 tatagcgagg gggtcatggt cggctaccgg gggtatgacg agctcggcct ccagccccgg
     3061 ttcccgttcg gccacggcct ctcgtacacg acgttcgagt acggcggtct ggaggtcgat
     3121 cccgcgccgg cagccgagga cgacgtcgtc gcgaccgtca gcttcgaggt ggcgaacacc
     3181 gggagcaggc ccggcgtcga ggtggcccag gtgtatgtcg gccggcttcc cggtccgacc
     3241 cggaccccgc tccgtcagct cgccggttgg gcccggatcg ggctcgatcc cggcgagcgc
     3301 cggcgcgtgt cggttgcgat cgcccggcgg tcagtttcgg tctgggatgt ggacacccac
     3361 gcctggcaga cgccggccgg ggacgtgtcc gtggaggtcg gccgttcgtc gcgcgacctg
     3421 cgcctgagcg cccgcgtcgg ggtccgctga tcagccgaac gccggcccgt catcgaccgg
     3481 gccggcgttc gtcgttccgc gcaggggact ggaccgccta cttgacggac ccggccagga
     3541 gaccctggac gaagtagcgc tggaggctga agaacaccag gagcggcagg aacatcagca
     3601 ggaacgcgct cgctgcgagg atgtgccact gcgatccgta cgtcccgagc agcgtcagca
     3661 cctggacggt gatcggtttc gtgccctggc cctgggcgaa gatcagcgcg acgagcaggt
     3721 cattccagac ccacaggaac tgaaagatcc catacgccgc gatcgccggc acggacaggg
     3781 gcacgacgat ccgccggaag atcccgaacg acgaggcccc gtcgatccgg gccgcctcga
     3841 tcaggtcctt cggcagggtg atgaagaaat tcctgagcag gaagattccg aagggcagag
     3901 cgaacgcggt atgggccagc cacaggccgg caaagccctg ggtcagtccg aacggcttca
     3961 tcagctggag aaccgggatg aacgcgacct ggatcggcac catcaggagc gcgatcgtga
     4021 tcaggaacag cgtgtcccgg aacgggaatc gcagccacga aaaggcgtag gccgccagtg
     4081 atgccagaaa gatcggaagg aacgtcgacg ggagcgtgat gatcaggctg ttgatgaagg
     4141 ccttgaggat gccctggccg ttcagcacct cgacgtagtt gtcgagcgtg aagcggagcg
     4201 tcccgagggt ctcccaccag cccgtcgacg cgatgtcgtt gcgcggccgg aacgacgtga
     4261 tcagcagccc gatcgtcggc accagccaca cgatcgccac gatcccgagc gcgatgtgga
     4321 tcggcgtccg gttcagcatc ttgatgatcc ggccactcag ggtgcggggc ttcgccccgg
     4381 ccatcccgac accggtgagg gcatcctggg tcatgtgacg gactccgtcc ggaacctgcg
     4441 gatgttgaag atcatgatcg ggatcaggat cacgagcatg acgacggcga cagccgcgcc
     4501 cttgccgaac tgattgcccg ggaaggcttc ctgatacatc gtgaaggcga tgacccggct
     4561 cgctgccccc ggccctccct gggtcatcac gtagatcagg tcgaagagct tgatcacacc
     4621 gacgatcagg gtgacggcca ggaccgacat cggcaggctg accatcggca ggatgatctt
     4681 caggaagacc tggaactcgt tcgcgccatc cgagcgggcc gcttcgagga tctcggtcga
     4741 gatcgacttg atcgccgccg agaggatgac cgtcgcgaac ccgaccccgg cccagacgcc
     4801 gacgaagatc agggccgcgt tgacgaagtt cgtgtcaccg aggaacgcga tcggcttgat
     4861 cccgaagaca ccgaggaagg cgttcaggaa gccctgatca gggttcgccg agtagacgaa
     4921 ggtccagatc acgccgagag cggtcgcggc gatcgcctgg gggatgaaga cgatcgcctt
     4981 gatctgcttc tcgtagcgca cccgggtggc catcacggcg atgatcaagc ccagggccag
     5041 cacgatcccc gtgtagaaga tcagccacag gacgttgttc cagattgccc cgctcggcgg
     5101 gaaggtcttg aagttgatga agttcggatc gttcgtcagc aggttgatgt agttcgtgaa
     5161 ccccacgaac tccgagaact ggctgttggc acgaccccga aagaagctga gcgcgatcgt
     5221 gtagatcgcg ggaaacacca ggaagatggc catgagaagc atggccggag cgaggaacag
     5281 gaccgcaatc ggctcgcgct ccctcgctcc gcccttcttg gccgccttga ccggcaccgc
     5341 cggcgggaca gcttgtggag cgacagccat gagccctcct cgcgcttgga atcgaacgag
     5401 ggcggacccg tgaagtccgc cctcattcag ctcagtcgac tattcggcag cccatgcggc
     5461 ctgctgggcc gtgttgaacg tgctgaccaa tccgtcgatc ggatcgccct tgatgacgct
     5521 ctggagcatc gccccgaagt ccgggccacc ggccggcagg aggtcttgcc cgtcgaaccg
     5581 gacggccgcg gcgttggcga cctgagcggc ctccttcttc gcggactcgt tcggatagat
     5641 gtcggccgag acgcccttga tcggcgagat gatgatcccc gtcttcgccc aggtggtccc
     5701 ggcatcgacg gtcgtcatgt actgcaggaa ctccttgacg cccggcttgg cggtcaaggc
     5761 ggcgatgaca tcgccaccga tcgtgacgcc gcccggattg ccgaacccgg ggaagtcgaa
     5821 ccacccgatc gtgtcgcccc acttgaggtc cttgttggta tcgccggtgg caatgccgta
     5881 gacaaagccg ccctcgtagt acatcgccgc atccggggcg ggcttgaaga cctggccgat
     5941 gccgtcggtg aaggcggccc cgagggcacc gttgatcccg ccgacgacat tgtcctcgat
     6001 caggacctgc ttcatcgtct ccatcgctgt cttgacggtc gggtcggtcc agtcgccgtc
     6061 cttgctgaag agcttcgtat acgcgtcagg accggcctgg cggatgtaaa tgctctcgaa
     6121 ccagtcggtc agcgtccacg agtccttggc gccgagcccg agcggcttga tgttcttgga
     6181 tttgagcgta ctgatcacgg tcttgaactc gtcccacgtc ttcggctcgg ccgtgacgcc
     6241 ggccgtcttg aagaggtccg gacgatagaa gaccgtgctc ttgctgttga acttgaccat
     6301 caggccgtac tgcttgccgt caacctggcc gatttccagg atgcccgggg cgtagttgct
     6361 ggcgagggcg ctgacgtcga tcccgaggga cgcgaagtcc ttgatcgagc cgtccttcgc
     6421 gaagctgcga aggaagccga tgcccggcat gatcgcgaca tcaggcgggt tgccgcccgc
     6481 gatggcggtc cggagaaccg tcgcgtagtc ggtgcgctgg gccacgtagt tggccgtgat
     6541 gccggtcttg gccttgaagt cggccaggac cgtctggaag gcggtctcct cggcgccgcc
     6601 ccacagggag gtgaccgtga tcgagccgcc ggtggcggcc tgcgagcccg tcgcagccga
     6661 gctgccggat gtcggagccg tcgacgcggg tgggttggat gcgccgccgc cggtgctcgg
     6721 ggcgggggtg ccgccgccgc cgcatgcgga gacgatcaaa gctgccgcaa tgggcagggt
     6781 caggaaccgc gggaatttgg ccatcggcct tctcctcctc cttgctgacg ccccggcccg
     6841 gccggcggtc catccccgct ctcgcgatcg aggatcgtca gacgaaatct tgcccacaac
     6901 ccgtgggacg tgctgcccgg cggtcgccgg gtgtgtaggt cgacgacgtc cgatcggctg
     6961 ccggcggatc tccctgtcgc cccgctggcg gagccagcgc gggcaggggg caggcctgca
     7021 gtggtgatcg ggcgcgtcgc ggtgccggat cgagtatccg acggcgagac tagccgcgca
     7081 ttctcggcac tgtcaacgtt acagcgatat cgcgcgtcca cgtggcggct caggcgggtg
     7141 gggcgaagcg gatccggacc gtccggatct cccacggccc gagggcgagg cgaagggagc
     7201 catcgggctc gagcaccagg tccgcaccca gccggccgag aaggtcgatg tgctgggcgg
     7261 ccgcgaggcg ccggccggag atcgacgcca cggtggccgt gtcgatctcg gcgacgacgc
     7321 gcagctccag ctcgtcgccg tcgccgctcg ctgctggtcg tcgccggaga gcggagagga
     7381 cgacgccctg gccgtcgatc cggacgcctg cggccggggc gtcgggctcc gggtcagccg
     7441 accccggacc gccccggccg acggcggtca cgaacgggag ctggtaggcc tcggccgctc
     7501 gcaggacgtc ggcgtcgctc cacgagccgg cgtgcggcag gacggcgaac gtgaaccgcc
     7561 acggcccgat gatctgggca gcgggaacgg cgacctccgg gccggccggg tcctcgcgga
     7621 acgggttgtc gttgcggctg atcaggcccg tcgagcggag gaccgtcaac gccagctcgc
     7681 gcccgtcgac gagctcgtac tcggtgacgt ggtcgagcag gaccgtcgcc cccgccgcgt
     7741 ggacgaagcc gtgggccggg aacgtcgggg tcgggacctc gccgtggccg gcctcctcgg
     7801 tcaggccccg gtcgacgacg gcaaactggc cctcggccga ggagtggtcg gtcgccgcgg
     7861 gcagcggcag gtgccagcgg acgcgatggt cgcgggccgg attcgtgaac gcgacgacga
     7921 cccgcacgaa cggctcgccg gcccgtaact cgaccgtggt cgtgacctcg accggcgccg
     7981 tggcatcgct ccgggatgag ccgtcggtgg ccagcccgac cggccagtcg tagctcgcga
     8041 cgacctcgat cgagccgcgg atcgggccgc gctcctgggc ggcggtgacg acgattcgcg
     8101 gccggtcgac aacccggtcc gtggccggcg gcccgtagtt gtacgtgtcg ccgaagtcgc
     8161 cgccatccac gagccggccg gcgccggtca gccggacccc gccgccgtcg atcgtgagcg
     8221 tgccgtcgtc ggcgacctgg acgctgacga gcccgttgcg gatcgcgcga tcctcgacca
     8281 tgaccgcggc gttcgacgga accgcggctt ggcccgcgtc gaccgggcgc acgagcgcac
     8341 cgccgagcgg cggcgcaggc acggccgcga ggagccggcg ccggtcaccc cgaacaaccg
     8401 ccacgtccca gatcgcgtcc gggtcgaggc cgctctcggc ggccacggcg tcgaggatcg
     8461 tgtcgacgtc gaagtcgacc ggcgcggcgg ggtcgtccat ccgcaggatc agctccggtg
     8521 ccgacccgtc gcgcacgatt tcgtggccat cgagcgacga gccgaacagc tcgcggccgt
     8581 ggagccgccg ggcgaacagc tcgggcagct ggcggccggt catccggagc cggcggagga
     8641 cggtctcggg gcgctcgacg agctgggtgg ggacgcgccg gccatcgccg agctcgagct
     8701 cgaccgcggg ccacgcggcc ggcacggcca ggtcgagctc gacgacatcg gatcggtcgt
     8761 gtggcgatgg gttcaccacg accgtcgtgc cacgcggggc cctcgcggcg aggggccgga
     8821 gtgccgtcgc gaggaccccg cggccgatct gctcggcctc ggcgaagcgg gtcaggacct
     8881 gggcgacgac cgcgtcctgg gagcagccgc agatcgaatc gtgggcggag ttgtcgacga
     8941 gccggcgcca ggcgagctca agcagccgct ccggccaggc gccgccatga agggccgcga
     9001 acggctccgc gtagcgctcg agcatccgtt cggcgcgtcc ggctgccgcc ttgatgtcga
     9061 tccgggccga cgtgacgttc atgagcatgt tggcccgcgc cgctgagcgc agctctccga
     9121 tccagaccgg cgtcggcgcg ggatcgggcg cggcggggtc ggcgagcgcc gcatcgaacg
     9181 ccccgatgta ggtgctcagg gtctcgatcc ggacggtgat gtcgtcgcgc tgggcattgg
     9241 cgtccgcgac gatccgggcc aggcgcgggg aggggaccgc gtggtcggtg ccgtacatcg
     9301 cgaggatcga gcgatcgccg tagaacgccc ggctggcctc gacgtagcgg gcgactttgt
     9361 cggcgagccg gtcggggatc gccagcaggt acgcgccgtt gccgtagccg ccgacgaggt
     9421 actccgcccg gaccgccgag ccgtccggcg agcgccaggt gaaggcgtgc cggtcgatcg
     9481 cggcgggcac gccccgccag acgacggcgt ggcggatgcc ggctcgccgc aggatctgcg
     9541 gcatctgggc gacgtggccg aacatgtccg gcaggtagcc gaccggcatc gcggccccga
     9601 acgcctccgc cgcgtgccag ccgatctcga ggttgcgcac gatcgtctcg ccggacacga
     9661 ggaactcatc catgaggatc tgccagggcc cgatcgcgag ac
//
LOCUS       DATAIW010000469         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_288364, whole genome shotgun sequence.
ACCESSION   DATAIW010000469 DATAIW010000000
VERSION     DATAIW010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288364"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VHM48_13955"
     CDS             <1..752
                     /locus_tag="VHM48_13955"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2756567.1"
                     /translation="GIVLGSGLGGLADDLEDPVAIPFAELPGWPAATAPGHVGRLLLG
                     RLDGTPVVMLQGRFHVYEGNDPGLVVQPVLLFRKLGVEIVVLTNAAGGVDPGFGPGTL
                     MVIADHLNLTGRTPLLGPNSDKIGPRFQDLTDAWSPRLRTALKAAGAAEGVALEEGVY
                     AGLLGPQYETPSEVRMLRGLGADAVGMSTVLECIAARWAGLEVCGVSLVTNAGAGYSG
                     EPLSHEEVMAAGAEAGPRLARVIRRFVSGTG"
     gene            complement(759..1328)
                     /locus_tag="VHM48_13960"
     CDS             complement(759..1328)
                     /locus_tag="VHM48_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756568.1"
                     /translation="MRRRGAIPGFVGVAASLVLGCGLGGRGDPPPSPSTIFEPAVIGL
                     IEATETGTLTSTVRLGDGPTYSLPDNSPVRLPNGGGVLKPGDLMLAGPSVPPTWWVDL
                     PPRLLNRLPGATGPEVIPDGKCWLIDGGAFDEGAAIHFSSGLLLQKAEGFHVVQTWIE
                     DPLPARASDSLCVDVGGHVTTLEFIWEPF"
     gene            complement(1328..>2069)
                     /gene="thyX"
                     /locus_tag="VHM48_13965"
     CDS             complement(1328..>2069)
                     /gene="thyX"
                     /locus_tag="VHM48_13965"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2756569.1"
                     /translation="YYVPLIYTACRTCYSELTPEDIFERAATGKIEQAKMQKLISGVI
                     ESGHGSTIEHVVFTFGISGVSRTLSHQLVRHRAGVAFDQQSQRYVTFKGASTMLPATI
                     QDADPDLREAYEEQIAGSMDLYSQLVGAGIPGEDARFVFPNATRTNLVMTTNLRALIH
                     MSGLRLCTMAQWEIRRLFQLIRHEIFQVSPFLGSFLAPKCVALGYCDEMGNRDEHCPI
                     RPHKDNVLNAWAEKSKAAKAAGRELTVR"
BASE COUNT          297 a          711 c          743 g          318 t
ORIGIN      
        1 tcgggatcgt cctcggctcc ggcctcggcg gactggccga cgacctcgag gatccggtcg
       61 cgatcccgtt cgccgagctg cccggctggc cggccgcgac cgcccctggc catgtcgggc
      121 ggttgttgct cggccggctc gacgggacgc ccgtcgtcat gctccagggc cgcttccacg
      181 tgtacgaggg caacgacccg ggcctcgtcg tccagcccgt gctcctcttc cgcaagctcg
      241 gcgtggagat cgtggtcctg acgaacgccg ctggcggcgt cgatcccggc ttcgggcccg
      301 ggacgctgat ggtcatcgcc gaccacctca acctgacggg tcgcacgccc cttctgggcc
      361 cgaactccga caagatcgga ccgcgattcc aggacctgac cgacgcctgg agcccgcggc
      421 tccggaccgc gctgaaggcg gccggcgcgg cggagggcgt ggcgctcgag gagggcgtct
      481 acgccggcct cctcgggccg cagtacgaga ccccgtccga ggtccggatg ctccgcggcc
      541 tcggcgccga cgccgtcggg atgtcgaccg tcctcgagtg catcgccgcg cgctgggccg
      601 gcctcgaggt ctgcggcgtg tcgctcgtga cgaacgccgg cgcgggctac agcggcgagc
      661 cgttgagcca cgaagaggtg atggctgccg gcgccgaggc agggccacgg ctcgcgcgcg
      721 tgatccggag gttcgtctct ggcaccgggt gaggtcgcct agaacggctc ccagatgaac
      781 tcgagcgtcg tgacgtggcc accgacgtcg acgcaaagcg agtcgctcgc tcgcgctggg
      841 agcgggtcct caatccatgt ctgaacgacg tggaagccct cggccttctg cagcaacagc
      901 ccgctcgaga agtggatcgc ggcaccttcg tcgaaggcgc cgccgtcgat cagccagcat
      961 ttgccatccg gaatgacttc gggcccggtc gcccccggca gtctgttcag aagccgcggg
     1021 gggaggtcga cccaccacgt gggtgggacg ctgggccccg ccagcatgag gtcgccgggc
     1081 ttgagcacgc cgccgccgtt cggcagccgg accggggagt tgtccggaag cgaatacgtc
     1141 ggcccatcgc caaggcggac cgtgctcgtc agcgtgccgg tctccgtcgc ctcgatcaag
     1201 ccgatcacag cgggctcgaa gatcgtcgac ggggacggcg gcgggtcacc tcggccgccg
     1261 aggccgcagc cgagcacgag cgaagcggcg acgccgacga accccgggat cgccccccgc
     1321 cgccgcatca gcgaaccgtc agctcccggc cggccgcctt cgcggccttc gacttctcgg
     1381 cccaggcgtt gaggacgttg tccttgtgcg gccggatcgg gcagtgctcg tcgcggttgc
     1441 ccatctcgtc gcagtagccg agggcgacgc acttcggggc gaggaagctg ccgaggaacg
     1501 gcgagacctg gaagatctcg tggcggatca gctggaagag ccggcggatc tcccattggg
     1561 ccatcgtgca gaggcgcagc ccggacatgt ggatcagggc ccgcaggttg gtcgtcatca
     1621 cgaggttcgt gcgggtcgcg ttcgggaaca cgaagcgggc atcctcgccc gggatccccg
     1681 cgccgacgag ctggctgtag agatccatcg agcccgcgat ctgctcctcg taggcctcgc
     1741 ggaggtccgg gtcggcgtcc tggatcgtcg ccggcagcat cgtcgaggcg cccttgaacg
     1801 tgacgtagcg ctggctctgc tggtcgaacg cgacgccggc tcgatggcgg acgagctggt
     1861 gtgacagcgt ccgcgacacg ccgctgatgc cgaaggtgaa gacgacgtgc tcgatcgtgg
     1921 agccgtggcc ggactcgatc acgccggaga tcagcttctg catcttcgcc tgctcgatct
     1981 tgccggtcgc ggctcgctcg aagatgtcct ccggggtgag ctcggagtag cacgtccggc
     2041 aggcggtgta gatcagcggg acgtagtac
//
LOCUS       DATAIW010000470         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_228335, whole genome shotgun sequence.
ACCESSION   DATAIW010000470 DATAIW010000000
VERSION     DATAIW010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228335"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHM48_13970"
     CDS             complement(<1..536)
                     /locus_tag="VHM48_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2756570.1"
                     /translation="MTKIDRAELSVFSAPDPDGGGRGLDRAGLLAAAEAIQDEVVTIR
                     RRIHRRPEVGLDLPHTQSVVLEELAKLGIQGTKGTALSSVVAVIEGGLPGPTVLLRGD
                     MDALPLHEDTGLDFTSEIDGAMHACGHDTHVAMLLGAARLLQDRRADLPGRVILMFQP
                     GEEGMGGAKTMIEEGLLDA"
     gene            complement(725..>1869)
                     /locus_tag="VHM48_13975"
     CDS             complement(725..>1869)
                     /locus_tag="VHM48_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756571.1"
                     /translation="PPGSPSHPPTFAEEAEWTGLDWSAPLRSEPSTTASDLIATPSGF
                     VAVGQVQTAAGGSVAAAWTSPDLRSWTRTLLDAPAIGDSSLESVRRTDAGLVAIGLSG
                     VLQCVPPEGEGQVCDPLPIAIWTSSDGASWTQERQPAPLVGVRVASVASDGETVFLVG
                     DAGWTRPGIWRSSDGAIWASAHPQPDTFADAHFDAVAAIPGRGWVVTGSTGGSEPVCC
                     AGHNADATPAAWSSADGITWEPALVVAAVASRGDVVGPVFAGRDGMVAWSLGEQANDF
                     GWQSPDGRTWTALPKPVGNAITPRAGDGLRIIGTTFDGEDRVAEWISTDGLRWNRLAA
                     SGDVASRPTWSEGRTADAAFLLAGGVAWIGRDVDGAPLLWWSTPAR"
BASE COUNT          275 a          699 c          667 g          228 t
ORIGIN      
        1 gcgtcgagga gcccctcctc gatcatcgtc ttcgccccgc ccatcccctc ctcgccgggc
       61 tggaacatca ggatcacccg gcccggcagg tccgcccgcc gatcctggag gagccgcgcc
      121 gcgccgagca gcatcgccac gtgggtgtcg tggccgcagg cgtgcatcgc gccgtcgatc
      181 tcggaggtga agtcgaggcc ggtgtcctcg tggagcggga gcgcgtccat gtcgccccgg
      241 aggaggaccg tcggcccggg caggccgccc tcaatcaccg ccacgacgga gctcagcgcc
      301 gttcccttcg tcccctggat cccgagcttc gcgagctcct cgaggacgac gctctgggtg
      361 tggggcaggt cgagcccgac ttccggccga cgatgaatcc ggcgccggat cgtgacgacc
      421 tcgtcctgga tcgcctcggc ggcggccagc agcccggccc gatcgaggcc tcggccgccc
      481 ccatcggggt cgggggcgct gaagacggac agttcggcgc gatcgatctt cgtcatggcg
      541 ccgagaatac gacgaggacc cgtttcgctc atcggagagc cggtgggacg cagcccgacg
      601 ccaattgcgc gacgatcctg cagggctgcg gcgcgaccat ccccaccaac gtcaccacga
      661 cggccgggtg aaccgataac cgctaccacc gcggcaggga cggcgcggcg cggcgggtgc
      721 ggcgtcaccg ggccggcgtc gaccaccaga ggagcggcgc gccgtcgacg tcgcggccga
      781 tccatgcgac gccgccggcc agcaggaatg cggcgtccgc ggtccggccc tccgaccacg
      841 tcggccggga cgcgacatcc cccgacgccg caagccgatt ccagcggagg ccgtcggtcg
      901 agatccattc ggcaacgcgg tcctcgccat cgaaggtggt cccgatgatc cggagaccat
      961 ctccggcccg tggtgtgatc gcgttgccga ccggcttcgg cagagcggtc caggtccggc
     1021 cgtccggcga ctgccagccg aagtcgttgg cctgctctcc gagcgaccac gcgaccatgc
     1081 cgtcgcggcc cgcgaagacg ggaccgacca cgtcgccgcg tgacgcgacc gcggccacga
     1141 ccagggccgg ctcccacgtg atcccgtcgg ccgaggacca cgcagcgggc gtcgcgtcgg
     1201 cgttgtggcc ggcgcagcac accggctccg atccgccggt ggacccggtc accacccagc
     1261 cgcggccggg gatcgccgcc acggcgtcga agtgggcatc agcgaacgtg tccggctggg
     1321 ggtgcgcgct cgcccagatc gcaccgtccg acgagcgcca gatgccggga cgggtccagc
     1381 ccgcgtcacc gacgaggaag accgtttcgc cgtcgctcgc gaccgaggcg acgcggacgc
     1441 cgacgagggg agcaggctgc ctctcctggg tccacgacgc gccgtcgctc gatgtccaga
     1501 tcgcgatcgg cagcggatcg cagacctgcc cctcgccttc gggcgggacg cactggagga
     1561 ccccggacag cccgatcgcg acgagcccgg cgtccgtccg gcggaccgat tcaagcgacg
     1621 aatcgccgat cgccggggca tcgagcaggg tccgcgtcca ggatcggaga tcgggcgagg
     1681 tccacgcggc ggccaccgac ccgccggccg cggtctggac ctggccgacc gcgacgaacc
     1741 cgctcggcgt cgcgatcagg tccgaggcgg tcgtggacgg ctcggaacgg agcggcgccg
     1801 accaatcgag gcccgtccat tcggcctcct cggcgaaagt cggcggatgg gacggcgacc
     1861 ccggagggc
//
LOCUS       DATAIW010000471         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_229011, whole genome shotgun sequence.
ACCESSION   DATAIW010000471 DATAIW010000000
VERSION     DATAIW010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229011"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1464)
                     /locus_tag="VHM48_13980"
     CDS             complement(157..1464)
                     /locus_tag="VHM48_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX2756572.1"
                     /translation="MMGEPGGRATDGGASQLRDVEQLAVDAVNAAWTADVAAQLVRVP
                     SVTGDEGAVQDLVAALLADIGVAVERLEPPLDAVRADPDWPGDEMPRDRLPIVLGRIG
                     RPGGRRLVLVGHVDVVPTGDLATWSHDPWAAERDGDRLYGRGAVDMKGGVASILGAVR
                     ALAGAGLADRLAGEVLVAIVPSEEDGGQGMLAAIRAGATGDMAVITEPTGLDVVIAHA
                     GAITFKLTVPGRAAHASVRREGISALDKLQTLVRALEADETARNAAETEPLMTVHGLP
                     YATIIGKVSGGAWASSVIDRIEAEGRYGVKLGQTWRDAERDLQRCIDDANESDPFLRD
                     HPATVELTGGKFSSSRVPADHPLPVGLAAVVHDVLGRDPELAGEPYGADMRLLVNEGN
                     TPTVIFGPGDKRVAHSADEWVSVAEVADCARTLAAWLVRELVP"
     gene            complement(1461..2318)
                     /locus_tag="VHM48_13985"
     CDS             complement(1461..2318)
                     /locus_tag="VHM48_13985"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX2756573.1"
                     /translation="MSQAYGGQSNVAPLRDALLKAPGEAFGRAFDNPAYGFLHHCDLE
                     VAKREHAAFANLLTSLGTKVHLLDVEIDSPDLLYAYDPLIVTDKGAIPLRPGKPNRVP
                     EAAAIEGWTSARGIPTAGRIEAPGTIEGGDTFWLRPDLFCIGRTLRTNQAGADQLATL
                     VGGDVRIFDVPYWKGPAELVHLMSVISPIADDLAVVFTPLIPVGLWQLLGDLGYRLID
                     VPEEEYPTLGVNVLAVRPGVVIAAEGNPVTAAAMAAHGVEVHTYPAFEIGMNGSGGPT
                     CMTRPILRG"
     gene            complement(2315..>2624)
                     /locus_tag="VHM48_13990"
     CDS             complement(2315..>2624)
                     /locus_tag="VHM48_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2756574.1"
                     /translation="RYLAGKLSAIAGVRMLGLTAEDAMDRRTPTLAITVAGVSPREVA
                     VRLGKRGIAVWDGDFYATGLIERLGLAPDGVVRIGLTHYNTRAEIDRLAAELTEIAAS
                     "
BASE COUNT          357 a          960 c          936 g          371 t
ORIGIN      
        1 catctggtcg acctcggcgc tggcgagcct cggttcgggt ttggtcgtcg cggccgccgg
       61 ctacacgacg ctcggcctcc ttggcgtcgc gatcgtcgcc ggccctgcgc tcctgctgct
      121 cagtcggcgg catgccatcg ccgccggcgg cgcttcctag ggcacgagct cccggacgag
      181 ccacgcggcg agggtccggg cgcagtcggc cacctcggcg accgagaccc actcgtcggc
      241 ggagtgcgcg acgcgcttgt caccgggccc gaagatcacc gtcggcgtgt tgccttcgtt
      301 gacgaggagg cgcatgtcgg cgccgtacgg ctcgccggcc agctccgggt cgcgaccaag
      361 cacgtcgtgg acgaccgccg cgaggccaac gggcagcggg tggtccgccg gcactcgcga
      421 ggacgagaac ttgccacccg tcagctcgac cgtcgcggga tggtcacgca ggaacgggtc
      481 ggattcgttg gcgtcatcga tgcagcgctg caggtcgcgc tccgcgtcac gccacgtctg
      541 gccaagcttc acgccgtagc ggccctcggc ctcgatcctg tcgatgaccg acgacgccca
      601 cgcgccaccg gacaccttgc cgatgatcgt ggcgtagggc aggccgtgga ccgtcatgag
      661 cggctctgtc tcggccgcat tccgcgcggt ctcgtcggcc tcgagggctc ggacgagcgt
      721 ttggagcttg tcgagcgcgg agatgccctc gcggcgcacc gacgcgtgcg cggcgcgacc
      781 ggggacggtc agcttgaacg tgatcgcgcc cgcatgagcg atcacgacat cgaggccggt
      841 cggctcggtg atgaccgcca tgtcgccggt cgcgcctgcc cggatcgcgg cgagcatgcc
      901 ctgcccgccg tcttcctcgg acggcacgat cgcgacgagg acctcgcctg cgaggcggtc
      961 cgccagaccg gccccggcga gcgcccgcac ggcgcccagg atcgaggcga cgccgccctt
     1021 catgtcgacc gcgccgcgac catacagccg atcgccgtcg cgctcggcgg cccacgggtc
     1081 gtgcgaccag gtcgcgaggt cgccggttgg gacgacgtcg acgtgcccca cgaggacgag
     1141 ccggcgaccg ccgggccggc caatccgccc aagcacgatc ggcaaccggt cccgcggcat
     1201 ctcgtcgccc ggccagtccg ggtcggcgcg gacggcgtcg aggggcggct ccagccgctc
     1261 cacggcaacg ccgatatcgg caaggagcgc cgcgacgagg tcctggaccg cgccctcgtc
     1321 gccggtgacg ctcgggacac gcacgagctg cgcggcgaca tcggccgtcc aggcggcgtt
     1381 tacggcgtcg accgcaagct gctcgacgtc gcggagctgc gaggcgcccc cgtccgtcgc
     1441 gcggccgcca ggctcgccca tcatccgcgc aggatcggcc gggtcatgca cgtcggcccg
     1501 ccggagccgt tcatcccgat ctcgaacgcc ggatacgtgt gcacctcgac accatgcgcc
     1561 gccatcgcgg ccgcggtgac cggattgccc tcggcggcga tgacgacgcc cggccgcacc
     1621 gccagcacgt tgacgccgag cgtggggtat tcctcctcgg gcacgtcgat cagccggtag
     1681 ccgagatcgc ccaggagctg ccacagcccg accgggatca gcggcgtgaa caccaccgca
     1741 aggtcgtcgg cgatcgggct gatcacggac atgaggtgga cgagctcggc cgggcccttc
     1801 cagtacggga cgtcgaagat ccgaacgtcg ccgccgacga gcgtcgcgag ctggtcggcg
     1861 ccggcctggt tcgtgcgcag cgtccggccg atgcagaaca ggtccggccg cagccagaac
     1921 gtgtcgccgc cttcgatcgt gccgggcgct tcgatccgac ccgccgtggg gatgccgcgc
     1981 gcgctcgtcc agccctcgat cgccgcggct tcggggacgc ggttcggctt gccgggccgc
     2041 agcgggatcg cgcccttgtc cgtgacgatc agcgggtcgt acgcgtacag caggtccggg
     2101 ctgtcgatct cgacgtcgag caggtggacc ttcgtaccga gcgatgtgag caggttcgcg
     2161 aacgccgcat gctcgcgctt ggcgacctcg aggtcgcaat ggtggaggaa gccgtaggcg
     2221 gggttgtcga acgcgcgccc gaacgcctca ccgggcgcct tgagcagggc atcgcgcagc
     2281 ggcgcaacgt tgctctggcc gccgtatgcc tggctcaaga cgcggcgatc tccgtcagct
     2341 ccgccgcgag ccggtcgatc tcggcgcgcg tgttgtagtg cgtcagcccg attcggacca
     2401 cgccgtcggg cgcgaggccc aggcgttcga tgagcccggt cgcgtagaag tcgccgtccc
     2461 agaccgcgat cccgcgcttc ccgagccgca ccgcgacctc gcgcggcgac acgcccgcga
     2521 cggtgatcgc cagggtcggc gtccgccgat ccatggcgtc ctccgcggtg agcccgagca
     2581 ttcggacgcc ggcgatcgcg gagagcttcc ctgcgaggta tcgg
//
LOCUS       DATAIW010000472         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_289216, whole genome shotgun sequence.
ACCESSION   DATAIW010000472 DATAIW010000000
VERSION     DATAIW010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289216"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VHM48_13995"
     CDS             complement(<1..248)
                     /locus_tag="VHM48_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756575.1"
                     /translation="MNRLIRHHSARRGLVGLAVFALVVSGVRPGGNSADGGSRVGGPA
                     AIRSLAGRPVVSGAVGADAALVSARLPRAALAPTAPLTT"
     gene            525..>1802
                     /gene="speB"
                     /locus_tag="VHM48_14000"
     CDS             525..>1802
                     /gene="speB"
                     /locus_tag="VHM48_14000"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX2756576.1"
                     /translation="MAFDFARLNGWDRDWEVFSDFDLPTYVGPTTFMNLPWITDPAEL
                     RARNVDVAIIGAPFDDAVSHRPGARFGPRAIREAQYTSGSVHSLQLGVEPFEILNVVD
                     AGDANIVPAWIERAHALIHRKVREVAATGAIPIVLGGDHSITWPSATAVAEVRRPGSI
                     GIVHFDAHADTANDDWGVLAGHGTPMRRLIESGAVDGRNFIQVGLRGYWPPVETFEWM
                     KEHGLRWHFMTEVEERGADAVIDDAIAEALDGPDSIYLSLDIDVVDPGLAPGTGTPEP
                     GGFMTRELLRAIRRIVGQVDLAGMDIVEVSPPYDHAETTALIASSAATGGRFATPGRA
                     PARGSRVRLSTQPAGPRTATPRRPDDLAPDRAHRRPADGDESGPGPALRSRPRRGPGR
                     RQSLPRARDPDRGASPRARRGQWPDRRPARARRS"
BASE COUNT          257 a          672 c          635 g          238 t
ORIGIN      
        1 gtcgtcagcg gagcggtcgg cgcgagggcc gctcggggca gcctggcgga cacgagggcg
       61 gcgtccgcgc cgaccgctcc gctgacgacc ggccggcctg ccagcgaccg gatggccgcg
      121 ggtccgccga ccctcgagcc gccgtctgcg gagttgccgc ccgggcggac gcccgacacg
      181 acgagggcga acacggccag gcccacgagg ccacggcgag cggaatggtg gcgaatcaag
      241 cgattcaagg cgttctcccg gcggcgcgga atggcggttc gatcgacctt gcgcacggga
      301 cccgcggtcg ctctgccatc ctgccacgca tcggcgaacc acggaagccg ccccgtgatc
      361 gggtgttagg atggccgcgg cgcgccccgc tggacgctcg gtacgaaccg gcggcgcccc
      421 cagcgagacg acctcattcc gccgccgctc ccgccggggt ggcgcgatcg tctcccggag
      481 gacccgtggc cgagcgtccc cgcgaacccc gtcccgatcc caccgtggcg ttcgactttg
      541 cccgcctgaa cggctgggat cgcgactggg aggtcttcag cgacttcgac ctgccgacct
      601 acgtcggacc gacgacgttc atgaatctgc cgtggatcac cgatcccgcc gagctgcggg
      661 cccgcaatgt cgacgtcgcg atcatcggcg cgccgttcga cgacgccgtc agccaccggc
      721 caggggcgcg gttcgggccg cgggcgatcc gcgaggcgca gtacacctcg ggctcggtcc
      781 attccctcca gctcggggtc gagccgttcg agatcctgaa cgtcgtcgat gccggcgacg
      841 cgaacatcgt cccggcctgg atcgagcggg cgcacgcgct gatccaccgc aaggtccgcg
      901 aggtcgccgc gaccggggcg atcccgatcg tcctcggcgg cgatcactcg atcacctggc
      961 cgagcgccac ggcggttgcc gaggttcgcc ggccggggag catcggcatt gtccatttcg
     1021 acgcccacgc cgacaccgcc aacgacgact ggggcgtcct cgccggccac gggacgccga
     1081 tgcgccgact gatcgagtcc ggtgcggtcg acggccggaa cttcatccag gtcggcctgc
     1141 gcggctactg gccgccggtc gagacgttcg agtggatgaa ggagcacggc ctccgctggc
     1201 acttcatgac tgaggtcgag gaacgtggcg ccgacgccgt gatcgacgac gcgatcgccg
     1261 aggcgctcga cgggcccgat tcgatctacc tgagcctcga catcgacgtc gtcgatccgg
     1321 gtctcgcgcc gggcacggga acgcccgagc ctggcggctt catgacccgc gaattgctcc
     1381 gagcgatccg ccggatcgtc ggccaggtgg atcttgcggg catggacatc gtcgaggtct
     1441 cgccgccgta cgaccacgcc gagacgacgg cgctgatcgc ctcgagcgcc gcgacgggcg
     1501 gccgattcgc tacgccgggc cgggcaccgg cgaggggcag ccgagtccgg ttgtcgacgc
     1561 aaccggccgg gccacgaacg gccacgcccc ggcgcccgga tgacctcgcg ccagaccgcg
     1621 cccaccgacg ccccgccgac ggcgacgagt cgggccctgg cccggctcta cgatctcgac
     1681 ctcgtcgagg acccgggcga cgtcaatctc tacctcgagc tcgcgacccg gaccgggggg
     1741 ccagtcctcg agctcgccgc gggcagtggc cggatcgccg tcccgctcgc gctcgccggt
     1801 ca
//
LOCUS       DATAIW010000473         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_199257, whole genome shotgun sequence.
ACCESSION   DATAIW010000473 DATAIW010000000
VERSION     DATAIW010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199257"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2505)
                     /locus_tag="VHM48_14005"
     CDS             complement(67..2505)
                     /locus_tag="VHM48_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2756577.1"
                     /translation="MADHARAVIIGGGVGGTSIAYHLAERGWTDIVLVDRAELTSGST
                     FHSAGLVGQLRGSVTLTTMMMYGADLYRRLGDETGVDPSWHEVGSLRLASSTARFEEL
                     QRQASWAKTFGLPLELITASEARNRFPLMSTDGVLGAVWLPTDGWLDPSGLAMALAAG
                     ARRRGVTIRTHTRVVGIGVERGRVTGVEVELRDGSRETIAADVVVNAGGMFAPEIGRL
                     AGVTVPIIPMAHQYLFTEAIDGVHAGLPQLRDPDNLVYFREEVGGLCMGGYERNPAPW
                     SLDGVPPDFNGKLLAPDTPRFEEIMAGAVRRVPAIADAGVSRIINGPEGFTPDNEFIL
                     GESEVGGFFVAAGFSAHGIAGAGGIGRQVASWIVDGQPELDLWKMDIRRFGPAYRSQG
                     YTLARSIENYATYYDIHYPNEERASGRPLRMAPTYEQLLALGAAFGEKSGWERPNWFE
                     SNAAAGDESLRPRGWAGEHWSPAIGAEALATRRAAGLFDESSFAKLEIAGPGALAFLE
                     RMCAGRIDRPVGSIVYTQLLDPRGGIQADLTVTRVADQAFMLVTGTAFGEHDAAWLRS
                     HLPDDGSVLLRDLNAGRVCLGLWGPRARDILASVTRDDVSDTAFPFLTARELSVGHVP
                     ALALRVTYVGELGWELYAGTEYGRALWSTLWEAGRPHGLVAAGYRAIDALRLEKGYRA
                     WASDITPDETPYDAGLGFAVALAKGVDFIGRDALVAANAGGPRKRLRCLVLDDPRSVC
                     LGNEPVRIDGAIVGRVTSGGYGYAVERSIAYAYLPQDAPIGTRGEIDVFGEWIGFEVM
                     REPLYDPANAKIRS"
     gene            complement(2606..3619)
                     /locus_tag="VHM48_14010"
     CDS             complement(2606..3619)
                     /locus_tag="VHM48_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2756578.1"
                     /translation="MNGRGSGARQVGLLVVVFGATIVLLVGGRALLGRAQSERPVGSA
                     ATPGTSGVAVIASPTSPSGSASPDGSGPPTIVLTGAGDIADCTLKGAAQTSDLLIGQA
                     GSIFTAGDNAYEDGSAANFGDCYGPTWGRVKDRTILTAAGNHDWNTDGAAGYLAYFGA
                     AAAPQGVTWYSTDLGAWHVVVLDSDCARVGGCDDASPQGRWLAKDLAASEARCTLAIW
                     HHPRFSSGEHGNDREVAPFWDQLHAAGAELVVNGHDHDYERFAPQDAAGKVERPGGLR
                     EIVIGTGGGELRAFHTLAANSEFRQAGTWGVLRLTLHPANYDWEFLPVDSDLADSGST
                     PCH"
BASE COUNT          556 a         1397 c         1346 g          538 t
ORIGIN      
        1 aggccggcgc tccagtcggg gccgtagccc gcggactggt ccatcgcggc cgggctcatg
       61 acgcgctcac gaacggatct tcgcattcgc cgggtcgtac agcggctcgc gcatcacctc
      121 gaagccgatc cattcgccga acacgtcgat ctcgccgcgg gtcccgatcg gcgcgtcctg
      181 cggcaggtag gcgtaggcga tcgaacgctc gaccgcgtag ccgtagccac ccgatgtcac
      241 ccgtccgacg atcgcgccgt cgatcctgac cggctcgttg ccgaggcaga cggagcgcgg
      301 atcgtcgagg acgaggcaac ggagacgctt gcgcggcccg ccggcgttcg ccgcgaccag
      361 cgcgtcgcgg ccgatgaagt cgacgccctt ggcgagggcg accgcgaagc cgagcccggc
      421 gtcgtacggc gtctcgtcgg gggtgatgtc gctcgcccag gcgcggtagc ccttctcgag
      481 ccgcaaggcg tcgatcgccc gatagcccgc agcgaccagg ccgtgcggcc ggcctgcctc
      541 ccacagcgtc gaccacagcg cccggccgta ttccgtcccg gcatagagct cccagccgag
      601 ttcgccgacg taggtgacgc ggagggcgag cgccgggacg tggccgacgc tcagctcgcg
      661 cgcggtcagg aacggaaagg cggtatcgct gacgtcatcg cgcgtgacgc tcgcgaggat
      721 gtcgcgggcc cgcggacccc acagcccgag gcacacccgg cccgcgttga gatcacggag
      781 caggaccgag ccgtcatccg gcaggtggct gcggagccag gccgcgtcgt gttcgccgaa
      841 cgcggtcccg gtgacgagca tgaaggcctg gtcggcgacg cgggtcacgg tcaggtcggc
      901 ctggattccg ccgcgcgggt cgagcagctg ggtgtagacg atcgacccga ccggccgatc
      961 gatccggccc gcgcacatcc gctccaggaa ggcgagggct cccgggccgg cgatctcgag
     1021 cttggcgaag gagctctcgt cgaagagacc ggccgcacgc ctggtggcga gcgcctccgc
     1081 gccgatggcc ggcgaccagt gctccccggc ccagccgcgt ggccgcaggc tctcgtcacc
     1141 ggcggccgcg ttcgactcga accagttcgg ccgctcccag ccggacttct cgccgaacgc
     1201 agcgcccagc gccaggagct gctcgtaggt cggtgccatc cggagcggtc ggccggacgc
     1261 gcgctcctcg ttcgggtagt ggatgtcgta gtaggtcgcg tagttctcga tcgagcgggc
     1321 cagcgtgtag ccctgcgagc ggtaggccgg tccgaagcgc cggatgtcca tcttccagag
     1381 gtcgagctcg ggctgaccgt cgacgatcca gctcgcgacc tggcggccga tcccgccggc
     1441 cccggcgatg ccgtgggccg agaacccggc ggcgacgaag aacccaccga cttccgattc
     1501 gccgaggatg aactcgttgt ccggggtgaa gccctccggg ccgttgatga ttcgtgacac
     1561 tcccgcatcg gcgatcgcgg gcactcgccg cacagcgccc gccatgatct cctcgaaccg
     1621 cggcgtgtcg ggcgccagca gcttgccgtt gaagtccggc ggcacgccgt cgagcgacca
     1681 cggtgcggga ttccgctcgt aaccgcccat gcacagcccg ccgacctcct cgcggaagta
     1741 gacgaggttg tcgggatcgc gaagctgcgg caggcccgcg tggacgccgt cgatcgcctc
     1801 ggtgaagagg tactggtggg ccatcgggat gatcgggacg gtgacgccgg ccagcctgcc
     1861 gatttccggc gcgaacatcc cgccggcgtt gacgacgaca tcggccgcga tcgtctcgcg
     1921 cgagccgtcg cggagctcga cctcgacgcc ggtcacccgt ccccgctcga cgccgatccc
     1981 gacgacgcgg gtgtgggtcc ggatcgtgac acccctccgg cgggcgcccg cggcgagggc
     2041 catggccagc cccgacgggt cgagccagcc gtcggtcggc agccagacgg cgccgaggac
     2101 gccgtcggtc gacatcagcg ggaagcggtt gcgggcctcc gaggccgtga tcagctcgag
     2161 cggcaggccg aatgtcttcg cccagctcgc ctggcgctgg agctcctcga accgggccgt
     2221 cgacgacgcc aaccgcagcg acccgacctc gtgccacgac ggatccacgc cggtctcatc
     2281 gcccagacgg cgatacaggt cggcgccgta catcatcatc gtcgtcaggg tcaccgagcc
     2341 gcggagctgg ccgacgagcc cggccgagtg gaacgtggat ccggatgtga gctcggcgcg
     2401 atccacgagg acgatgtcgg tccagccgcg ttcggcgagg tggtacgcga tcgaggtccc
     2461 gccgactccc ccgccgatga tcaccgcccg ggcgtggtcc gccatccgtt cccgttcctc
     2521 gtgtcgccgt tgacgccgct gactccccgc cggccgccgc gacgatcgac gctcagccta
     2581 tcgggtcggt cggccgccgt actggtcagt ggcacggcgt gctgccggag tccgcgagat
     2641 cgctgtcgac cggcaggaac tcccagtcgt agttggccgg gtggagcgtc aggcgcaaca
     2701 cgccccaggt gccggcctgg cggaactcgc tgttggcggc cagggtgtgg aacgcccgca
     2761 gctccccacc gccggtcccg atcacgatct cgcggagccc gcccggccgc tcgaccttgc
     2821 ccgccgcgtc ctgcggcgcg aaccgctcgt agtcgtggtc gtggccgttg acgaccagct
     2881 ccgcaccagc cgcgtggagc tggtcccaga acggggcgac ctcgcggtcg ttgccgtgct
     2941 cgccggagct gaaccgcggg tggtgccaga tcgcgagggt gcaacgggcc tcgctcgcgg
     3001 cgaggtcctt cgccagccag cgaccctgcg gcgacgcgtc atcgcagccg cccaccctgg
     3061 cgcagtccga atcgaggacc acgacgtgcc aggcgccgag gtcggtcgag taccaggtca
     3121 cgccctgcgg cgcggcggcc gctccgaagt acgccaggta gccagcggcg ccgtcggtgt
     3181 tccagtcgtg gttgccggca gccgtcagga tcgtccggtc cttgacccgc ccccaggtcg
     3241 ggccgtagca gtcgccgaag ttcgctgcgc tcccgtcctc ataggcgttg tcgcccgccg
     3301 tgaagatcga tcccgcctgg ccgatgagga ggtccgaggt ctgggctgcg cccttcagcg
     3361 tgcaatccgc gatgtccccg gcgccggtca ggacgatcgt cggcgggccc gagccgtccg
     3421 gcgaggccga tccggaaggc gaggtcggtg aggcaatgac cgccacgccg gacgtgccgg
     3481 gcgtggctgc cgagcccacg ggtcgctcag actgcgctcg ccccagcagc gcccgaccgc
     3541 cgacgaggag cacgatcgtc gcgccgaaca cgacgacgag cagcccaacc tgtcgcgccc
     3601 ccgaaccacg gccgttcatc cgggcaagcg taccgtccgg aggccggcag ccggcccccc
     3661 tgctattcgg tcgcctcgcg cagcgcccgc tcaaatgccg gggtcgtcgc gttccggatc
     3721 agccgctcga aatgctcact ggcgtaggcg acgaagtcga cgtcgagaat gctgatcccc
     3781 tgctggagga caccccacat cgcctcgcgg aagtcggaca tcgtcctcat cagggtc
//
LOCUS       DATAIW010000474        14447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_229715, whole genome shotgun sequence.
ACCESSION   DATAIW010000474 DATAIW010000000
VERSION     DATAIW010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14447
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229715"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..269)
                     /locus_tag="VHM48_14015"
     CDS             complement(102..269)
                     /locus_tag="VHM48_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2756579.1"
                     /translation="MLPDRAAYPALRTADPDRLRRFYEDVLGFTVRDENRRTEGRARR
                     SGPIRRAMSAS"
     gene            313..2232
                     /gene="aspS"
                     /locus_tag="VHM48_14020"
     CDS             313..2232
                     /gene="aspS"
                     /locus_tag="VHM48_14020"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX2756580.1"
                     /translation="MTDSPPLDRGAKDAPADRLATPYRTHTCGALRDSDAGTTARLSG
                     WVHRRRDHGQLIFLDLRDRHGITQVVVDRTDQPDAHAVASRVRSEFVVTVDGDVAARL
                     AGTENPRLPTGAIELRARDVRILSEAKTPPFYINDPDAPIDEALRLRYRYLDIRREAM
                     TRRLLLRSRLVQAIREVHHAHGFVEVETPNLIKSTPEGARDFIVPSRLQPGSVYALPQ
                     SPQQLKQLLMVGGIDRYFQIARCFRDEDLRGDRQPEFTQLDLEMSFVDEEAVTAFVEA
                     MVIEVCRATTPERPFQAIPFPRFTYDEAIERFGSDKPDLRFGMELVDLGPRLVGADGT
                     PASGFRVFDETLAAGGRVKAIVAPGMGAATRKEIDELTVRAKRFGAKGLAHLAVEPGG
                     EIRGPIVKFLSADLARGLVDEAAAGEGDLVLIVADTAAVAADVLGRLRSELGAKLGLA
                     DPDVLAFCWINRFPMYQWDAENERWDATHNPFSGVLPEDEELLTTLDGDPSKPSPNDP
                     AGRARAMQYDIALNGWELGGGSIRIHRRDLLARSFALQGQSDEGMKEKFGAVLEAFEY
                     GPPPHGGIALGIDRWAMILANQTNIREVMAFPKTQSGTDLMLEAPSPPDPAQYAELGL
                     RFVGLPGRTPGGEAG"
     gene            2240..3199
                     /locus_tag="VHM48_14025"
     CDS             2240..3199
                     /locus_tag="VHM48_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2756581.1"
                     /translation="MPPAAADSGLPAVLAAIAARPRLAALLGATCIAFSGIFYRWAEV
                     SPSTGTVFRALFGLPLLGLVAWLEHRRYGGLPVRTVRLALLAGVFFAGDLMFWHHAIA
                     YVGAGLATVLGNLQVLVVGVVAWLAFGERPSRNTFIALPIVLLGVALISGAIGGRAFG
                     ADPGLGVAFGLATAFCYAGYLLIIRRGGRDLRRPAGPVAIATVSTAAVAVLVGMIVGD
                     LDPTPAPASLAWLAILGLTSQSLGYLLISLSLSRLPAVLTSIILLAQPVMTVGLAIVL
                     LHEAPTPTQLLGVALVIGGIAVATGGGVAASRIRRTGQAKAGA"
     gene            complement(3472..4176)
                     /locus_tag="VHM48_14030"
     CDS             complement(3472..4176)
                     /locus_tag="VHM48_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX2756582.1"
                     /translation="MTVAAVILAASAESALADADGSPAVRRIADAAWAGGATPIVVVS
                     FDPDGSVAAALAGAAVTLAEPAPPEHGPAAQMARGADVARAEIDDTDAVLLWPARMTW
                     VGPETVTSLIELHGTTPGTLLRPTWAGEAGWPVLLPLDLLPALRSVAADRMPLDVLED
                     LAVAGAPERLVELGDPGVAIDGGTPRADLPPYIGPEEPLVGHVHEWGAAQADAPDDGP
                     LEGPALAPYSPAGEPG"
     gene            4553..6505
                     /gene="typA"
                     /locus_tag="VHM48_14035"
     CDS             4553..6505
                     /gene="typA"
                     /locus_tag="VHM48_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX2756583.1"
                     /translation="MPSDATTVSTDPGPAAGTRSTRADLRNVAIVAHVDHGKTTLVDA
                     MLRQTGTFRANETVVDRVLDSGDLEREKGITILAKQTTVDHAGIRLNIVDTPGHADFG
                     GEVERSLLMVDSVLLLVDAAEGPLPQTRYVLQKAMARHLPVVVAVNKIDRADARPAEV
                     LDEIYELFIDLGADAHQIEFPVIYTNAKLGTATRSLDEPATNLKVLLDVLVAVTPPPT
                     YEEGHPLQLLVTNLSANDYVGRMAVGRIWNGRIRTGQRITVLREEASDTAGAIEPGRI
                     VTISGTVTALQTAHGIDRIDIDDAGPGDIVSVAGLPEVTIGDTLTDPADPRPLPRLDV
                     DAPTLSMTFGVNTSPLSGREGKFVTSRQIKARLDKEVLGNVSIEVYSTETGDTFEVRG
                     RGELQLAVLLEQMRREGFELTASRPEVLLKSVNGEIQEPYERITIDIPPDYIGAVQEA
                     LAARKGRLEQMTTDADGRVRMEFVLPVRGLIGYRGQLLTDTRGTALLHQIGEGYGPWA
                     GEVTHRTNGVLVADRTGTSNAFGLFNLEERGELFIGAGVEVYEGMIVGECSRSGDMNV
                     NATKTKQLTNIRTHSHDESLRLTPARPITLESAIEFIAGDELVEITPLSIRLRKRSLS
                     EHDRRRARGKEEDLRRAAEREATGTD"
     gene            complement(6534..6875)
                     /locus_tag="VHM48_14040"
     CDS             complement(6534..6875)
                     /locus_tag="VHM48_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756584.1"
                     /translation="MDAADLAAGSALAAIAVAIGWFILGSLGRAADSMATLFVPPDRT
                     LGWPRGVQESDEPWGWRAAARSADDPTGPDGLDDEDWPTGLHVGPEPPRGTFVVAVMP
                     VAPIRLVVRPH"
     gene            complement(7025..7303)
                     /locus_tag="VHM48_14045"
     CDS             complement(7025..7303)
                     /locus_tag="VHM48_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756585.1"
                     /translation="MPTTDTMPTPETMSEPMDTTTEVAMDGDWIASPVDDTTYDLIMA
                     LASKLEAIDTYQVYAEDGQAELWRELATDERRHAERLFNELKQRIAAA"
     gene            7537..8721
                     /locus_tag="VHM48_14050"
     CDS             7537..8721
                     /locus_tag="VHM48_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2756586.1"
                     /translation="MAIYLDHAATTPLRREVLDAMLPFLTEDYGNPSSAHGFGRRARA
                     ALDEAHRRVAGRIGAEAREIVFTSGGTEALNLGLKGAAWAGKARGHRIVTSAVEHHAV
                     GHTLRYLEKFGFEVVELPVDRYGRVDPDHVEAAISDKTILVSIMLANNEVGTIQPIAE
                     IGRRVHAHRGVLFAVDAVQAGAHVELDVETLGVDLLALSAHKFEGPKGVGALYLRHGT
                     HIVVQQQGGSQERHRRGGTENVAGAVGMAAAYDLACMERPETVKRLRRQRERLQKAVL
                     AVDGTELTGHPKERLANHLSLIARDTDGNALTMSLDLAGIAASVGSACTTGSTEVSHV
                     LAAMGYPDEEARGALRLSLGRTTSDAEIDEACVTVPRVVASMRTGQVVVAADPLGQGA
                     LA"
     gene            8718..9884
                     /gene="mnmA"
                     /locus_tag="VHM48_14055"
     CDS             8718..9884
                     /gene="mnmA"
                     /locus_tag="VHM48_14055"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2756587.1"
                     /translation="MSRILVAMSGGVDSSVAAALLHEQGHDVVGVWMRLHDVADSYSE
                     FKKSCCSLDAADDARRVAAQLGIPFYVMNLEREFDAGVLQPFLTAYLDGRTPSPCVDC
                     NTYVKFGALLGRARHLYECEAVATGHYARRDVGPDGWARLLQARDEDKDQTYFLYGLR
                     QDQLEHARFPLGELTKPEVRAVARSLGLATADKPESQEICFVPGGDYRDALRDRAGWT
                     PEPGPVLDADGARVGEHPGSAGFTVGQRRGIGIAADAPRYVSRIDPLTNTIQLGRRED
                     LETRIVPMSGVTFVAGEPPGGPASAFRAEVRIRHRGSLVPATVRPASAGDGGDRRGWV
                     AETDAPVWAAAPGQACVLYAGEVVLGGGRIEAAGRVDDGTPTWADRPREVVGAA"
     gene            9935..10219
                     /locus_tag="VHM48_14060"
     CDS             9935..10219
                     /locus_tag="VHM48_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756588.1"
                     /translation="MIGPAPVLALLVGIFHTALYVLIRGSAGGQLPLLIVAAFLGAWA
                     GDELGGRLGLDLLRIGDFRLVAASLLAWVGIGMVSIMAILGPTRRPKVGP"
     gene            10245..10508
                     /locus_tag="VHM48_14065"
     CDS             10245..10508
                     /locus_tag="VHM48_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756589.1"
                     /translation="MSEDRVPAPLEALVRAIAGGAHDDDDQRATTFARGLALGALVGA
                     AIAGSTIWQRRHGRVEVEVEVESRRDDVAADDPSAPGGRHLRP"
     gene            10624..11940
                     /locus_tag="VHM48_14070"
     CDS             10624..11940
                     /locus_tag="VHM48_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756590.1"
                     /translation="MRHASRAAILAAGFILTFGLAACGTNSGNSGAAGSQPAAGSQPA
                     AGSQPAGSTGGGSAAALSGNLTVWAMGNEGDLLGKLGDAFMKVNPGVKVSVTPVAWGE
                     AVAKLQTAIAGKTTPDVSQMGTDMMGQFGAMGGFEPVPADILPTNYVDSAWKTNIVDG
                     AAMGVPWYVETRLLYYRTDIATKAGITTPPATWDDLKAMAKAMKEQGGAKWGISLGTK
                     NWQEYLPFLWSNGGDVVDASGKPTLNSPQAVEALTFYNSFFKEGLTPKSVPEGFAIEA
                     AFVKGDNPMFFSGPWHLGLVKTAGGAGFEGKWAIAPMPKKTTSTSFLGGSNVVVYKNS
                     QNKDAAWAFVKFLSDPKTQALWYTTATDLPAVLSAWDDPTVKADPNVAMFGAQLKDTK
                     AQPVSATWSELATAINDTLEKMTTGGMEPQAAADQMQQKAESIGTK"
     gene            11942..12886
                     /locus_tag="VHM48_14075"
     CDS             11942..12886
                     /locus_tag="VHM48_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2756591.1"
                     /translation="MTAPALVRRNDAGRNTAPVASRHGWRQDLTGWAFAAPFVILFGI
                     FLALPILSAFILSFTSFGLRDIQNPIGTTFVGAKNYVDLLSDPKFWKALGNTAYFVVV
                     GVPATLVLGLAIANALSRGITRFRTAFRVGYYLPVITSIVAIAVVWRFLLNPDVGLIN
                     MLLQNVGITGPDWLANPTLAMPSIIAMAVWRNVGFAMVVFIAGMQAIPVVLYEAASID
                     GAGRWQSFRSVTLPMLRPTILFMLVITTIGYLQLFEEPFVMTKGGPLDATLSVTMYMY
                     QQGFAFFHQGYASAIAYVLFVIVAVIAFLQFKFLRSET"
     gene            12900..13778
                     /locus_tag="VHM48_14080"
     CDS             12900..13778
                     /locus_tag="VHM48_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2756592.1"
                     /translation="MTAIAARSADTPILSGRAGKRALGGWWIYLLLILGILLMVGPFL
                     WMLLGSLKPQAEFLVSPPTFLPHAATSDNYERLFGKLDFPRFFFNSSVVALAVTVGNI
                     VFCPMLGYALAKLPWHGKRLIMGLVLATLMVPAGITLIPNFVLMSNLGLVNTYPGLIL
                     PFLVGPFGVFLTRQFMYGIPDELLEAARIDGANEFRIFWQVVIPITVPVLATLGILTF
                     LGSWNSFLYPLVMAQEPKMYTLPVALATFATGQHQADHGMLMAGSVVLVVPVLIIFIL
                     FQRWITQGIATTGLKG"
     gene            13853..>14447
                     /locus_tag="VHM48_14085"
     CDS             13853..>14447
                     /locus_tag="VHM48_14085"
                     /inference="COORDINATES: protein motif:HMM:NF022773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HEX2756593.1"
                     /translation="MRPRPVRRLLSLIAIASLIAIGTAMIPAAATAAGNGNLTTAQRA
                     DLLGYARNTWASFVAMTDEDSGLPADSLSSDGTRSVQTSTTYIGAYLWSTVVAERLRI
                     IGHAEAVRRTEQTIGTLETMERHQASGQFFNWYDHRDGAKLTVWPATGDPMTPILSSV
                     DNGWLAAALQVVRNTVPEVSARAGAIYESMDFGFYYRP"
BASE COUNT         2101 a         5258 c         4943 g         2145 t
ORIGIN      
        1 cgacgatctc gtccgcgagc tcgaacggtc ccggacgagc caccaccacg ggaccgcagc
       61 gccgacgtga gcactccgga ccgctggacg cgcggcgcgg actacgaggc ggacatcgcg
      121 cgcctgatgg gtccggatcg ccgggcccgc ccctcggtcc gtcgattctc gtcgcggacg
      181 gtgaacccga gcacgtcctc gtagaagcgc cgcaggcgat cggggtcggc tgtccgcagc
      241 gccgggtagg cggctcggtc ggggagcatg gggcctcctg ggatcgggca actcccgtac
      301 gatacgcgga ccatgaccga ctcacccccg ctcgatcggg gcgcgaaaga cgcgcccgcc
      361 gaccgtctcg ccacgccgta tcggacgcac acctgcggcg cgctgcgcga ctcggatgcg
      421 ggcacgaccg cgcgcctctc cggctgggtc caccgccgcc gcgaccacgg ccagctgatc
      481 ttcctcgacc tccgggaccg ccatgggatc acccaggtcg tcgtcgaccg gaccgatcag
      541 cccgacgccc acgccgtcgc cagccgggtc cggagcgagt tcgtcgtgac cgtcgatggc
      601 gacgtcgcgg cccgcctcgc cggcaccgag aacccgcgcc tcccgaccgg ggcgatcgag
      661 ctccgggccc gcgacgtccg gatcctgtcg gaggcgaaga caccgccgtt ctacatcaac
      721 gatccggacg ccccgatcga tgaggcgctc cgcctccgtt accgctacct cgacatccgg
      781 cgcgaggcga tgacccgccg cctgctcctc cgcagccgcc tcgtccaggc gatccgcgag
      841 gtccaccacg cccacggctt cgtcgaggtc gagacgccga acctgatcaa gtcgacgccc
      901 gagggcgccc gcgacttcat cgtcccctcg cgtctccagc cgggctcggt ctatgccctg
      961 ccgcagagcc cccagcagct caagcagctc ctgatggtcg gcggcatcga tcgctatttc
     1021 cagatcgcac gctgcttccg cgacgaggac ctgcgcggcg accggcagcc ggagttcacc
     1081 cagctcgatc tcgagatgag cttcgtcgac gaggaggcgg tcacggcctt cgtcgaggcg
     1141 atggtcatcg aggtctgccg ggcgacgacg cccgagcggc cgttccaggc catcccgttc
     1201 ccacgcttca cctacgacga ggcgatcgag cggttcggca gtgacaagcc ggatctgcgg
     1261 ttcgggatgg agctcgtcga cctcgggccg cggctcgtcg gcgccgacgg cacgcccgcc
     1321 tcagggttcc gtgtgttcga cgagacactc gccgcgggcg gccgggtcaa ggccatcgtc
     1381 gcgccgggca tgggcgcagc gacgcgcaag gagatcgacg agctcaccgt gcgcgcgaag
     1441 cggttcgggg cgaagggcct tgcccacctc gcggtcgagc ccggcgggga gatccgcggc
     1501 ccgatcgtca agttcctgtc ggccgacctg gcccgggggc tggtcgacga ggccgcggcc
     1561 ggcgagggcg acctcgtcct gatcgttgcc gacaccgcgg ccgtcgccgc cgacgtgctg
     1621 ggtcggcttc gctcggagct cggggccaag ctcgggctgg ccgatccgga cgtcctcgcg
     1681 ttctgctgga tcaaccgctt cccgatgtac cagtgggacg ccgagaacga gcgctgggac
     1741 gcgacccaca acccgttcag tggcgtcctc cccgaggacg aggagctcct gacgacgctc
     1801 gacggcgatc cgtcgaagcc gtccccgaac gacccggcgg gccgcgcccg agcgatgcag
     1861 tacgacatcg ccctcaacgg ctgggagctc ggcggcggct cgatccggat ccaccggcgc
     1921 gatctcctcg cccggagctt cgccctgcaa ggccagtccg acgaggggat gaaggagaag
     1981 ttcggggctg tcctcgaggc gttcgagtac ggaccaccgc cgcacggcgg gatcgccctc
     2041 ggcatcgacc gctgggcgat gatcctcgcc aaccagacga acatccgcga ggtcatggcc
     2101 ttcccgaaga cccagtcagg gaccgacctg atgctcgagg cgccgtcgcc gcccgatccg
     2161 gcccagtacg ccgagctcgg cctgcgcttc gtcggactgc cgggtcggac gccgggcggc
     2221 gaggccgggt agcgcttccg tgccgcccgc cgccgccgac agcgggctcc cggccgtcct
     2281 cgccgcgatc gccgcgcggc cacgcctcgc ggccctgctc ggggcgacct gcatcgcgtt
     2341 cagcgggatc ttctatcgct gggccgaggt ctcgccgtcg accgggacgg tgttccgggc
     2401 gctgttcggg ctgccgctgc tggggctcgt cgcctggctc gagcatcgcc gctacggagg
     2461 cctgccggtg cggaccgttc gcctcgcatt gctggcgggc gtgttcttcg ccggggacct
     2521 gatgttctgg caccacgcga tcgcctatgt cggagcgggc ctcgcgacgg tcctcggcaa
     2581 tctccaggtg ctcgtggtcg gcgtcgtcgc ctggctcgcg ttcggcgaac ggcccagcag
     2641 gaacacgttc atcgccctgc cgatcgtcct gctcggggtc gccctgatct ccggcgcgat
     2701 cggcgggcgg gcattcggcg cggatccggg gctcggcgtt gccttcggcc tcgcgaccgc
     2761 gttctgctac gccggctacc tgctcatcat ccgccgcggc ggtcgcgacc tgcgccgacc
     2821 ggccggcccg gtggccatcg ccacggtctc gaccgcggcg gttgcggtgc tcgtcggcat
     2881 gatcgtcggc gatctggacc cgacgccggc gccggcgagc cttgcctggc tcgcgatcct
     2941 cgggctgacc tcgcagtcgc tcgggtatct cctgatctcg ctgtcgctgt cgcggctgcc
     3001 ggcggtgctc acctcgatca tcctgctggc ccagccggtc atgacggtcg ggctcgcgat
     3061 cgtcctgctc cacgaggctc cgaccccgac ccagctcctc ggcgtggcgc tcgtcatcgg
     3121 cgggatcgcc gtcgcgaccg gcggcggtgt cgcggcctcc cggatcaggc ggacggggca
     3181 ggccaaagcc ggagcctgac agggcttcga cgtcgatctc gccttcgacc tcggtggggc
     3241 gtcagacgcc tcgaccgcgc tcgaccgatc gacagcgcga gcagcttcga tcgcgggcca
     3301 tgtagcgctc gtcgcggaaa cctggcctca ccgcggccct cggctccgca ccgggcgctc
     3361 cgctcacctc ggaggatggg acgcctcggg cgcccggggg tcgttctggg tcgacggcaa
     3421 tcccgccgct tgacgtcggt cagtcgggtt ggcgcgtgct cggaccgccg gtcagccggg
     3481 ttcgccagcc ggggaatacg gtgcgagggc gggtccctcg agggggccgt cgtcgggcgc
     3541 gtcggcctgg gcagcgcccc actcgtggac gtgaccgacg agcggctcct ccggcccgat
     3601 gtagggcggg aggtctgccc gcggggtccc gccgtcgatg gcgacgcccg ggtcaccgag
     3661 ctcgaccagg cgctccgggg cgcccgcgac agcgaggtcc tcgaggacgt caagcggcat
     3721 ccggtcggcg gcgacggagc ggagggccgg caggaggtcc agcggcagga ggacgggcca
     3781 gcccgcctcg cccgcccagg tcggccggag gagggttccg ggcgtcgtgc cgtggagctc
     3841 gatgagcgag gtcaccgtct ccggtccgac ccacgtcatc cgcgccggcc agagcaggac
     3901 ggcgtccgtg tcgtcgatct cggccctggc gacatccgcg ccgcgggcca tctgggcggc
     3961 cggcccgtgc tcgggcgggg ccggctcggc gagcgtcacg gcggctccgg caagggcagc
     4021 ggcgaccgac ccgtccggat cgaacgacac gacgacgatc ggggtcgcgc caccggccca
     4081 cgccgcgtcg gcgatccgcc ggacggcggg cgagccgtcg gcgtcggcga gggccgactc
     4141 cgcagacgca gcgaggatga ccgcggcgac cgtcacgaac gtgcctcgga gatcacggcg
     4201 gctggcgttg ggctggtcac ggggccagtc tagaacgggc gcgcgactcc gggtagggac
     4261 gccccgccgg tgttccgatg cgggccttcc tcgtccagct cgggattctc gccccacggg
     4321 cacatgcgac gtgacggaca tccgcgaccg cccgaattga ccccgggccg cccgactcgg
     4381 tcagatccgg cccgcaccac gagagaatcg tgcatccgcg gggcacgaac gggcatccgt
     4441 ggtcgcatgt gcccgcccta cgagaatcgc attcccgccg accgccggtt tgggcggcgc
     4501 agcgttccat gcgagaatgg acgacccgaa tcatcaggag ccccgcccga ccgtgcccag
     4561 cgacgccacc accgtttcca ccgaccccgg cccggccgcc ggtacccggt cgacccgggc
     4621 cgacctgcgc aacgtcgcga tcgtcgccca cgtcgaccac ggcaagacga ccctcgtcga
     4681 cgcgatgctc cgccagaccg gcacgttccg ggccaacgag acggtcgtcg atcgggtcct
     4741 ggattcgggc gaccttgagc gcgagaaggg gatcacgatc ctcgccaagc agacgacggt
     4801 cgaccatgcc ggcatccgcc tgaacatcgt cgacacgccg ggccatgcgg acttcggcgg
     4861 ggaggtcgag cggtcgctgc tcatggtcga ctcggtcctg ctcctcgtcg acgccgccga
     4921 ggggcccctc ccgcagacgc gctacgtcct ccagaaggcg atggcccgcc accttccggt
     4981 cgtggtcgcg gtcaacaaga tcgatcgggc cgacgcccga ccggccgagg tcctcgacga
     5041 gatctacgag ctgttcatcg acctcggcgc cgacgcccac cagatcgagt tcccggtgat
     5101 ctacaccaac gccaagctcg ggacggcgac ccgctcgctc gacgagccgg cgacgaacct
     5161 gaaggttctc ctcgatgtcc tcgtcgcggt caccccgccg ccgacctacg aggagggcca
     5221 cccgcttcag ctcctcgtga cgaacctgtc ggccaacgac tatgtcggac ggatggcggt
     5281 cgggcggatc tggaacggcc ggatccggac cggccagcgg atcacggtcc tgcgcgagga
     5341 ggccagcgac acggccggcg ccatcgagcc cgggcgcatc gtcacgatca gtggcaccgt
     5401 gaccgccctc cagaccgcac atgggatcga tcggatcgac atcgacgacg ccgggcccgg
     5461 cgacatcgtc tcggtcgcgg ggctgccgga ggtcacgatc ggcgacaccc tcaccgatcc
     5521 ggccgacccg cggccgctgc cgcgcctcga cgtcgacgcc ccgaccctga gcatgacgtt
     5581 cggggtgaac acctcgccct tgtccggccg cgagggcaag ttcgtgacct cacgccagat
     5641 caaggcccgg ctcgacaagg aggtcctcgg caacgtctcg atcgaggtct actcgaccga
     5701 gaccggcgac acgttcgagg tccgcggtcg cggcgagctc cagctggccg tcctcctcga
     5761 gcagatgcgg cgcgaaggat tcgagttgac cgcgtcccgg cccgaggtcc tcctcaagag
     5821 cgtcaacggc gagatccagg agccgtacga gcgaatcacg atcgacatcc cgcccgacta
     5881 catcggtgcc gtccaggagg cgctcgccgc gcgcaagggt cgtctcgagc agatgacgac
     5941 cgatgccgac ggtcgcgtcc ggatggaatt cgtcctgccc gtccgcggcc tgatcggcta
     6001 tcgcggccag ctcctgaccg acacccgtgg gacggccctg ctccaccaga tcggcgaggg
     6061 ctacgggccg tgggccggcg aggtcaccca ccgcacgaac ggtgtcctcg tcgccgaccg
     6121 gaccggaacg tcgaacgcat tcgggctctt caacctggaa gagcgcggcg agctgttcat
     6181 cggcgccggc gtcgaggtct acgaggggat gatcgtcggc gagtgctccc gttcgggcga
     6241 catgaacgtc aacgcgacca agaccaagca gctgacgaac atccggaccc actcccacga
     6301 cgaatcgctg cgcctgacgc cggcccggcc gatcaccctc gagtcggcga tcgaattcat
     6361 cgcaggcgac gagctcgtcg agatcacccc gctctccatc cggctccgga agcgttcgct
     6421 gtccgagcac gaccggcgcc gcgcccgcgg caaggaggaa gacctccgcc gggccgcgga
     6481 gcgtgaggcg acaggaaccg actgaggccg aggatcgcct ggggcggcgg gcctcagtgc
     6541 ggtcggacga ccagccggat cggtgccacc ggcatgaccg cgaccacgaa cgtcccccgc
     6601 ggcggctcgg gccccacgtg gaggccggtc ggccagtctt cgtcgtcgag tccgtccggg
     6661 ccggtggggt cgtcagccga tcgtgctgcc gcccgccagc cccagggttc gtccgattcc
     6721 tggacgccgc gcggccaacc gagcgtccgg tccggcggga cgaacagcgt cgccatcgag
     6781 tcggcagccc gcccgagcga ccccaggatg aaccaaccga tcgcgaccgc gatcgcggcg
     6841 agcgcggatc cggcggcgag atcggctgca tccatggatc accccgtgtg atcgcccgat
     6901 tgtcctccac gcgcgcaacc tccgggtcga tcccgctcgg cccgacccgc tcggcccgac
     6961 ccgggcatga cgaacgggcg gctcggcgcc gcccgttcgc tccgtgctgg gtgggtgatg
     7021 gtcgtcaggc cgcggcgatc cgctgcttga gctcgttgaa cagccgctcg gcgtgacgtc
     7081 gctcatcggt cgcgagctcg cgccacagct cggcctggcc gtcctccgca tagacctggt
     7141 acgtgtcgat cgcctcgagc ttcgaggcca gggccatgat gaggtcgtag gtcgtgtcgt
     7201 cgaccggcga cgcgatccag tcgccgtcca tggcgacctc ggtggtcgtg tccatcggct
     7261 cggacatcgt ctcgggggtg ggcatggtgt cggtggtcgg catggagact cctcggtgtc
     7321 gggagtccga gcgtgcccgg cggtgccttg ccggggcccg gtgcgttccg tcagacggct
     7381 atcgaacagg tgtcagtcgg ccccgggccg ctctggcggc agctcgatgg cgcggtctgg
     7441 tcgcggcgcc aggccgtgtc gcgctccggg cgcgagtgcc gcccgaccgg acgccggcca
     7501 cggtcgggac cggacctcgg cgtacaatcg gcggccatgg cgatctacct cgatcacgct
     7561 gcgacgaccc ccctgcggcg tgaagtcctc gacgcaatgc tgccgttcct caccgaggac
     7621 tacggcaacc cgagctcggc ccacggcttc ggccggcggg cccgggccgc cctcgacgag
     7681 gctcaccggc gggtggccgg tcggatcggc gccgaggccc gcgagatcgt cttcacctcg
     7741 ggcggcaccg aggccctgaa cctcggcctg aagggcgccg cctgggccgg caaggcccgc
     7801 ggccatcgga tcgtgacgtc ggccgtcgag catcacgccg tgggccacac gctgcgctac
     7861 ctcgagaagt tcggcttcga ggtcgtcgag ctgccggtcg atcgctacgg tcgggtcgat
     7921 ccggaccacg ttgaggcggc gatcagcgac aagacgatcc tggtctcgat catgctggcc
     7981 aacaacgagg tcgggacgat ccagccgatc gccgagatcg gccgccgggt ccacgcccat
     8041 cgcggcgtcc tcttcgcggt cgacgccgtc caggccgggg cgcacgtcga gctcgacgtc
     8101 gagaccctcg gggtcgactt gctcgccctg agcgcccaca agttcgaggg gcccaagggt
     8161 gtcggcgcgc tctatcttcg ccacgggacc catatcgtcg tccagcagca gggcggatcg
     8221 caggagcgcc atcgccgcgg cgggacggag aacgtcgccg gcgccgtcgg catggccgcg
     8281 gcctacgacc tggcctgcat ggagcgaccc gagaccgtga agcggttgcg gcgccagcgc
     8341 gagcggctcc agaaggcggt cctcgcggtc gacggtacgg agctgaccgg ccatcccaag
     8401 gaacggctcg cgaaccatct gtccctgatc gcgcgggaca cggacggcaa cgccctgacg
     8461 atgtcgctcg atctcgcggg gatcgcggcg tcggtggggt cggcctgcac gaccggctcg
     8521 accgaggtct cgcacgtcct ggccgcgatg ggttatcccg acgaggaggc ccgcggcgcg
     8581 ctccgcctgt cgctcgggcg gacgacatcg gatgccgaga tcgacgaggc gtgcgtgacc
     8641 gttccgcggg tggtcgcctc gatgcggacc ggccaggtcg tggtcgccgc cgacccgctc
     8701 ggccaggggg ccctggcgtg agccggatcc tcgtcgcgat gtcaggcggg gtcgattcgt
     8761 cggtcgccgc cgcgctgctt cacgagcagg gccacgatgt cgtcggggtc tggatgcgcc
     8821 tccacgacgt cgccgacagc tactccgaat tcaagaagag ctgctgctcg ctcgacgcag
     8881 ccgatgacgc gcggcgggtc gcggcccagc tcgggatccc gttctacgtg atgaacctcg
     8941 agcgcgagtt cgacgccggc gtcctccagc cgttcctgac cgcctacctg gacggccgga
     9001 cgccgagccc gtgcgtcgat tgcaacacgt acgtgaagtt cggggcgctg ctcggtcggg
     9061 cgcgccacct gtacgagtgc gaggcggtcg cgacgggcca ctacgcccgg cgcgacgtcg
     9121 ggccggacgg gtgggcgcga ctcctgcagg cgcgcgacga ggacaaggac cagacctact
     9181 tcctctacgg cctccgccag gatcagctcg agcatgcccg cttcccgctt ggcgagctga
     9241 ccaagcccga ggtccgcgcc gtcgcccgct cgctcggtct cgcgaccgcc gacaagccgg
     9301 agagccagga aatctgcttc gtgcccggcg gcgactatcg cgacgcgctt cgcgatcgtg
     9361 ccggctggac gccggagccg ggtccggtcc tcgacgccga tggcgccagg gtgggggagc
     9421 accccgggtc cgccggattc accgtcggcc agcgacgggg gatcgggatc gccgctgacg
     9481 caccccggta tgtgagccgg atcgatccgc tcacgaacac gatccagctc ggccgccgcg
     9541 aggacctcga gacccggatc gtgcccatga gcggcgtgac gttcgtcgct ggggagccgc
     9601 cgggaggccc ggcctccgcc ttccgggccg aagtccggat ccgccaccgc gggtcgctcg
     9661 tcccggcgac ggtccggcct gccagcgcgg gcgacggggg agatcgccgc ggctgggtgg
     9721 ccgagaccga tgcgcccgtc tgggccgcgg cgccgggcca ggcctgcgtc ctgtacgccg
     9781 gcgaggtcgt cctcggcggg gggcggatcg aggccgccgg gcgcgtcgac gacggaacgc
     9841 cgacgtgggc ggatcggccg cgcgaggtcg tcggggcggc ctgacccggt ggttccggta
     9901 ggtcctgtcg gacgacgcgc gctatcctcg cggggtgatc ggaccggcgc ccgtcctcgc
     9961 cttgctcgtc gggatcttcc acaccgcgct ctacgtcctc atccgggggt cggccggcgg
    10021 ccagctgcca ctcctcatcg tcgccgcgtt cctcggagcg tgggcaggcg acgagctcgg
    10081 cgggaggctc gggctcgacc tgttgaggat cggcgatttc cgtctcgtgg cggcgtcgct
    10141 gctcgcctgg gtcgggatcg ggatggtgtc gatcatggcc atcctcgggc cgactcgccg
    10201 cccgaaggtc ggaccctgat ggcgaccgcg gcacggagcg agcgatgagc gaggatcgcg
    10261 tgccggcgcc gctcgaggcc ctggtccggg ccatcgccgg cggcgcccac gatgacgacg
    10321 accagcgggc cacgaccttc gcccggggcc tcgcgctggg cgccctcgtc ggcgccgcga
    10381 tcgcgggctc gacgatctgg cagcgtcgcc atggccgggt cgaggtcgag gtcgaggtcg
    10441 agtcgcggcg cgacgacgtc gccgcggacg atccgtccgc gcctggaggg cgccacctcc
    10501 gaccctgaca cgaacctgca acgacgggac cggaatacgc cgaccatccg cgagcgccgc
    10561 gctgccacga tcgcggccgg cgaggccgca gtctgcagcc tccggcctgt ggaggagtca
    10621 cagatgcgtc atgcctctcg agcggcgatc ctcgccgccg ggttcattct gaccttcggt
    10681 ctcgcggcgt gcggaacgaa cagtgggaac agcggcgccg ccggcagcca gcccgccgcc
    10741 ggcagccagc ccgccgccgg cagccagcct gccggctcga ctggcggcgg ctcggccgcc
    10801 gccctcagcg gcaacctcac ggtctgggcg atgggcaacg agggcgacct gctcggcaag
    10861 ctcggcgacg ccttcatgaa agtgaacccc ggcgtcaagg tcagcgtgac accggtcgcg
    10921 tggggcgagg cggtcgccaa gctccagacg gcgatcgccg gcaagacgac accggacgtc
    10981 agccagatgg gcacggacat gatgggccag ttcggggcga tgggcggctt cgagcccgtt
    11041 cccgccgaca tccttccgac caactacgtc gacagcgcct ggaagacgaa catcgtcgac
    11101 ggcgcggcga tgggcgtgcc ctggtatgtc gagacccggc tcctctatta ccggacggac
    11161 atcgcaacca aggccggcat caccacgcct ccggccacct gggacgacct gaaggccatg
    11221 gccaaggcga tgaaggagca gggcggcgcg aagtggggca tctcgctcgg cacgaagaac
    11281 tggcaggaat acctgccgtt cctctggtcg aacggcggcg acgtcgtcga cgcgagcggc
    11341 aagccgacac tgaacagccc gcaggccgtc gaggcgctga ccttctacaa ctcgttcttc
    11401 aaggagggcc tgaccccgaa gtcggtgccc gagggcttcg ccatcgaggc tgccttcgtg
    11461 aagggcgaca acccgatgtt cttctccggc ccgtggcacc tcggcctggt caagaccgcc
    11521 ggcggcgccg gcttcgaggg caaatgggcg atcgcgccga tgccgaagaa gacgacgtcg
    11581 acctcgttcc tcggcggcag caacgtcgtc gtctacaaga actcccagaa caaggacgcc
    11641 gcctgggcgt tcgtgaagtt cctgtccgat ccgaagacgc aggcgctctg gtacacgact
    11701 gcgaccgacc tcccggcggt cctgtccgct tgggacgacc ccacggtcaa ggcagatccc
    11761 aacgtcgcga tgttcggcgc gcagctcaag gacaccaagg ctcagccggt gagcgcgacc
    11821 tggagcgagc tcgcgaccgc gatcaacgac accctcgaga agatgacgac cggcggcatg
    11881 gagccgcagg ccgcggccga tcagatgcaa cagaaggcgg agtcgatcgg gacaaagtga
    11941 catgacggct cctgcccttg tccggcggaa cgacgcgggg cggaacaccg cccccgtcgc
    12001 ctcgcgccat ggctggcgac aggacctgac gggctgggcg ttcgccgccc cgttcgtcat
    12061 cctgttcggg atcttcctcg cgctgccgat cctctcggcg ttcatcctga gcttcacgag
    12121 cttcggcctg cgcgacatcc agaacccgat cgggaccacc ttcgtcggcg ccaagaacta
    12181 cgtcgacctg ttgtccgacc cgaagttctg gaaggccctc ggcaacaccg cctacttcgt
    12241 cgtggtcggc gtgcccgcaa ccctcgttct tggcctcgcg atcgcaaacg cactgagccg
    12301 cgggatcacg agattccgga cggcgttccg ggtcggctac tacctgccgg tgatcacaag
    12361 catcgtggcg atcgcagtcg tctggcgatt cctcctcaac ccggacgtcg gcctgatcaa
    12421 catgctcctc cagaacgtcg ggatcaccgg ccccgactgg ctcgcgaatc cgacgctcgc
    12481 catgccgtcg atcatcgcga tggccgtctg gcgcaacgtc ggtttcgcga tggtggtctt
    12541 catcgccggg atgcaggcaa tccccgtggt cctctacgag gccgcatcga tcgacggcgc
    12601 cgggcgctgg cagtccttcc gcagcgtgac cctgccgatg ctccgcccca cgatcctgtt
    12661 catgctcgtg atcacgacga tcggctacct ccagctgttc gaggagccgt tcgtcatgac
    12721 caagggcggg ccacttgacg cgaccctgtc ggtgaccatg tacatgtacc agcaggggtt
    12781 cgcgttcttc catcagggct acgcatccgc gatcgcgtac gtcctcttcg tcatcgtcgc
    12841 cgtcatcgca ttcctccagt tcaagttcct gcggtcggag acgtgatgga ggccgagcga
    12901 tgaccgcgat tgcggcacga tcggcggaca cgccgatcct ttccggcagg gccggcaagc
    12961 gcgccctggg tgggtggtgg atctatctcc tgctgatcct cgggatcctg ctcatggtcg
    13021 ggccgttcct gtggatgctc ctcgggtcgc tcaagcccca ggccgagttc ctcgtcagtc
    13081 cgccgacctt cctgccacac gccgccacga gcgacaacta cgagcggctc ttcgggaagc
    13141 tcgacttccc gcgcttcttc ttcaattcgt ccgtcgtcgc gctcgccgtg actgtgggca
    13201 acatcgtctt ctgtccgatg ctcggctatg cacttgccaa gctcccgtgg cacggcaagc
    13261 ggctgatcat gggcctcgtc cttgccacgt tgatggttcc ggccgggatc accctgatcc
    13321 cgaacttcgt cctcatgtca aacctcggcc tcgtgaacac ctacccaggc ctgatcctgc
    13381 cgttcctcgt cgggccgttc ggggtcttcc tgacccggca gttcatgtac ggcatcccgg
    13441 acgagctcct cgaagcggcc cggatcgacg gggccaacga gttccggatc ttctggcagg
    13501 tcgtcatccc gatcaccgtg ccggtcctcg cgacgctcgg gatcctgacg ttcctcggca
    13561 gctggaacag cttcctctac ccactcgtca tggcccagga acccaagatg tacaccctgc
    13621 ccgtcgcgct cgccacgttc gcgaccggac agcaccaggc cgaccacggg atgctcatgg
    13681 cgggctctgt cgttctcgtc gtgcccgtcc tgatcatctt catcctcttc cagcgctgga
    13741 tcacccaggg gatcgcgacg accggcctga agggctgact cgcccacgct tcacgcctga
    13801 gcccgacgcg tagcgaagcg gccggattgg ccgcaccacg gaggcacccg agatgcgccc
    13861 acgccccgtc cgccgcctgc tcagcctgat cgccatcgcc agcctgatcg cgatcgggac
    13921 ggcgatgatc ccggccgcgg ccaccgccgc cggcaacggc aacctgacga ccgcccagcg
    13981 cgccgatctg ctcggctacg cccggaatac ctgggcctcg ttcgtcgcga tgaccgacga
    14041 ggactccggc ctcccggctg acagcctcag ctcggacggc acgcgcagcg tccagacctc
    14101 cacgacgtac atcggtgcct acctctggag caccgtcgtc gccgaacggc tccggatcat
    14161 cggccacgcc gaggccgtcc ggcgcaccga gcagacgatc gggacgctcg agacgatgga
    14221 gcgccatcag gcgagcggcc agttcttcaa ctggtacgac catcgcgacg gcgcgaagct
    14281 gacggtctgg ccggcgaccg gggacccgat gaccccgatc ctgtcgtcgg tcgacaacgg
    14341 ctggctggcg gccgccctcc aggtcgtccg caacaccgtc cccgaggtct cggcccgcgc
    14401 cggcgccatc tacgagagca tggacttcgg cttctactac cggcccg
//
LOCUS       DATAIW010000475        11040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_199874, whole genome shotgun sequence.
ACCESSION   DATAIW010000475 DATAIW010000000
VERSION     DATAIW010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199874"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VHM48_14090"
     CDS             <1..283
                     /locus_tag="VHM48_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX2756594.1"
                     /translation="ETGITRRRSGFEAAAIALPRPLLVRLAAARSTLEAAASALAVLD
                     PQATLERGYAIVRRAADERILRAPDEAPVGTALAIRLAGGELAATAAER"
     gene            401..712
                     /gene="xseB"
                     /locus_tag="VHM48_14095"
     CDS             401..712
                     /gene="xseB"
                     /locus_tag="VHM48_14095"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX2756595.1"
                     /translation="MTAEPTATPPAGPSDAVPPTVDPAIATLSFDDAFAELRAAVAEL
                     EAGGLSLEDTIARTERAVALQRHCEKLLGEAELRVRQLVSRPGGGLEARDIAADEAIE
                     A"
     gene            complement(782..2134)
                     /locus_tag="VHM48_14100"
     CDS             complement(782..2134)
                     /locus_tag="VHM48_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2756596.1"
                     /translation="MQTDDQIAPAASFPATAILPDHPAYDEHRISFNGMLDRRPAVIV
                     PCTSTDEVVGAVRAARTAGLPIAVRGGGHSVAGHGVADGALVVSLGSMRDVTVDPERR
                     IAHVGGGAQWNDIDAAAFAHGLAVPGGTFGDTGVGGLTLGGGIGWLMPVAGLTCDNLI
                     EAEVVTADGSVVIAGPGGDEELLWALRGGGGNFGVVTRFTYRLTPVGPMFGGEIRYLP
                     EAAGEVLARVETLIARYPLATMPTLTIHRDPDLGPIVAFLLGIVDGSDPEPILELLRR
                     DLPVVDDDLGTRTYLDLQALAGILPFGLRHYWKGHFVRELDTSTFEALVAAVSAPDAV
                     GKAFVLIEGITGAGRTEPAGGAAFGQRAARWNASALSIWESPDEDEVAIGWARRVADL
                     LEPLSQSGGGYINYSPADESAERVVAAYGAERWERLVAVKRRHDPANVFRFNHNIRPD
                     "
     gene            2324..4384
                     /locus_tag="VHM48_14105"
     CDS             2324..4384
                     /locus_tag="VHM48_14105"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2756597.1"
                     /translation="MTTPILPTLQGPADLRRLTELELEQLAGEVRDAIIATVATTGGH
                     LGSSLGVVELTIALHRLLESPRDRIVWDTGHQAYPHKLLTGRFARFGTLRELGGIGGF
                     PRRSESPHDVFDGGHAGTGLSIGQGLATARDLHHGNERIAVVVGDAAITSGLSLEALN
                     DIGQRKTQLLIVLNDNEMSISPTVGGLSKYLSQIKLSGTWQSSKTAYDEFVGRIPAVG
                     PTVRELGQRLRASVVNFAQPGRLFEDLGITYIGLVPGHDLKALLSTFQQALELKGPVI
                     VHVRTQKGHGFRPAEADQVGFHGAALPPIAVPASTNGHVDGNGATPLAAVSAVPGLGS
                     MPTESMADDAAPASAIIRPAKNPNYTAVFAAELIERARADERIVAITAGMPTGTGLSM
                     FATEFPDRFIDVGIAEQHSVALATGLAMGGMRPVVALYSTFLQRAFDQTVHDVCQNDQ
                     PVLIAVDRAGLVGEDGTSHQGMFTLTAQRQLPNLVIASPKDEQELRSLLVSALGQDRP
                     FALHYPRDPGFGLAPVEPAAIPIGRGEMLREGTDLLFVGFGPIVARAVAAADALAAEG
                     WSVAVVNARFAKPLDRELILGQARDKKIIVTFEESVVTGGFGSGVLEAVEEARLVEPA
                     LREVPVKILGIPGGSFVDHGSVADLRRVLRLDANGLAAQVRETLDQLGARPASGAVVG
                     MGHR"
     gene            complement(4571..4969)
                     /locus_tag="VHM48_14110"
     CDS             complement(4571..4969)
                     /locus_tag="VHM48_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756598.1"
                     /translation="MLARPISLILAAGLLILIGLSGMAAGGGLLSTIANGSSTTADVR
                     AAALAFGTTMAVYGFAAVLAGVGLLIFRRWAWRLGLLLSVVGLVLLTGAQLAAGPDLI
                     LGFGVAVWVTTFVCLVAPDTRRAVAGPPSS"
     gene            5061..5906
                     /locus_tag="VHM48_14115"
     CDS             5061..5906
                     /locus_tag="VHM48_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX2756599.1"
                     /translation="MSPTTVRRRRQRLDQLVVERGLADSRARAQALILGGKVRTGDGD
                     GARLDRKAGDLVDPDVTISLIETDPYVSRGGHKLVAALDSFGIDPAGLVCLDAGASTG
                     GFSDVLLQRGAAQVHAVDVGRGQLADRVARDPRVVVHDRMNARTLTAETLGEPIELAV
                     IDVSFISLALVLGPIVSTLRSRPPAAIIALVKPQFEVGKGGTDHGVVRDPEAHRTVLQ
                     RVTAAAAGLGLGTRAVIASPILGPEGNREFLVHLQAGPSCAEVGERIDEAVAPTAAGD
                     GTGPP"
     gene            5903..6748
                     /locus_tag="VHM48_14120"
     CDS             5903..6748
                     /locus_tag="VHM48_14120"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX2756600.1"
                     /translation="MTIQRIGFAYNPTTEAANELRERAEGWCRMHRIEHWAAQAEDFD
                     LLCRELATTDILLVLGGDGTLLRAARAVIQVDVPLLGVNLGKVGFLSKVEATDLEGVL
                     AKLVAGEFTIDPRMALEARILPAGREADGEIHHALNDVVIARGALARVTRLDVAIGPT
                     HLATFIADGLIVASPTGSTGYSFSAGGPIVAPDSRNLIVTPIAAYLSAVRSVVVGPDQ
                     VIRCRIVDAAEAIVSIDGREDVVVAVGDVVEVRALVRPIRLVAPSGAQPFWDLFRQKV
                     ELLPS"
     gene            6798..8558
                     /gene="recN"
                     /locus_tag="VHM48_14125"
     CDS             6798..8558
                     /gene="recN"
                     /locus_tag="VHM48_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX2756601.1"
                     /translation="MRSGPAENADRGRAVGRLLELTVTDLALIERLRLELAPGLNVFT
                     GETGAGKSLLIDALGLAVGARADSTLVRHGAETARVSALFDRLPEPLIAAREVSVAGR
                     STARLDDEPVTAARLADVAGALVEIHGQHDQQRLLDERWQRDLLDAFGGHAVMRASVA
                     AAVERWRENRALLAALELDPRELARRLGLAEHEAAEIAAAQLRPGEADEIRVRLEAAQ
                     HGEAIARGSATLVDALAGEEQGARDVVAVALHEVRALARLDQRFVEIAERLAGLEAEL
                     DDVASTVRDLADSVDHDPAELARLADRLSTIYGLERRFGDDEEAVIAHGERAAAEAER
                     LRGVDDERAARQADDVRLLEQVAAVAADLSAARATAGRALAEDVGRVLAELGFPPDVF
                     EVGLGRRPAGRAEAAVEIDGDALAFDAAGIDQVVFRIAPNAGEPARSLARIASGGELS
                     RVALAIKEVLAEVDDTPSLVFDEIDSGIGGRSADPVGRSLWSLGRRHQVLCVTHLPQI
                     AAHADAHFQITKRERDGRTVTEVTRLDREGRIVELGEMLGGADADGAQGSTGGTATLA
                     SARELLDRAEAWRMRAGVSA"
     gene            8578..9504
                     /locus_tag="VHM48_14130"
     CDS             8578..9504
                     /locus_tag="VHM48_14130"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX2756602.1"
                     /translation="MPVTAAPPLERAIDDYLTYLRVERGLSPATIRAYRGDLTDFAAG
                     EGAATAWAAGPEAARRHLASRTRRGAKPPRDPGLAPTSLRRRAASIRGFYRFAYGDGL
                     IERDVAAHLDLPRQPRLLPETLTVAEVEALLDAAPDLRARALLELLYAAGLRVSEALG
                     LDREDLSTDGGFVRVIGKGDKERLVPVGDIALDWLERWMADGRPALLELAHVEPQRGG
                     PLFLGDRGRRLARQQAFTIVKVAARAAGLSDAVSPHTLRHSFATHLLEGGADLRIVQE
                     LLGHASISTTQLYTHVTGERVREVYAKAHPRA"
     gene            9523..10251
                     /locus_tag="VHM48_14135"
     CDS             9523..10251
                     /locus_tag="VHM48_14135"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2756603.1"
                     /translation="MAYLDNLLASGEQPLRVEHQHWFVVAADARYAFFAWIGAIALLI
                     VARMLPDFIPDVLGWGVVILVIGGLLYLGWQILRWQNEEFVVTSRRVLMTEGVVNKRV
                     IDSSLEKINDAILTESIFGRMFGFGDLEILTASESGISRLRMLRKADDFKRTMLDAKH
                     ELELELSGARPMPGPAIRTAAPPAPMQPTAAVPAAAPPAPAAAALSADDVTRTLANLA
                     DLRDRGAISAEEYEAKKADLLRRL"
     gene            10307..10960
                     /locus_tag="VHM48_14140"
     CDS             10307..10960
                     /locus_tag="VHM48_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2756604.1"
                     /translation="MFVQQHPVEALALIAFFLLLAFPVHEFAHAYAAYRLGDSTAKLF
                     GKLTLNPIAHFDQIGGSMLVISVLLTGFPFGFAQTPVNPRNLRGKYGEVIVAAAGPIS
                     NLLMAAAVAIPLRLLFPLTAGDQHIYVTEPELVLKLIVQYSILLGLFNLIPIPPLDGG
                     AIMLSLVSPRTAWQLRPMLAQYGFFLIILLIIPLGGQSILGRVLLPIVNAIYGVLVG"
BASE COUNT         1540 a         4024 c         3900 g         1576 t
ORIGIN      
        1 cgagacgggc atcacccgcc gcagatccgg gttcgaggcc gccgcgatcg ccctgccgcg
       61 gccgctcctc gtccgcctcg ccgccgcccg ctcgacgctc gaggcggccg cgtcggcact
      121 ggccgtcctc gatccgcagg cgacgctcga acggggctat gcgatcgtcc gtcgggccgc
      181 cgacgagcgg atcctccgtg cgcccgacga ggcgccggtc gggacggcgc tggcgatccg
      241 cctggccggc ggcgagctgg cggcgacggc ggccgagcga tgatcgacct cgtgctcgtg
      301 atcggcatcg cgatcgtctt cacaacgctc gggctgggcg ttggtatgct cgtctccggt
      361 cggatcacgg cctggatgga ccgcagcgac aaggagcagg atgaccgctg agccgaccgc
      421 gaccccgccc gccggcccca gcgatgccgt ccctccgacg gtcgaccccg cgatcgccac
      481 cctgtccttc gacgacgcgt tcgccgagct ccgagccgcc gtcgccgagc tcgaggcggg
      541 cgggctgtcg ctcgaggaca cgatcgcccg gaccgagcgg gcggtcgccc tccagcgcca
      601 ctgcgagaag ctcctcggtg aggccgagct gcgtgtccgc cagctcgtca gccgtccggg
      661 cggcgggctc gaggcccgcg acatcgcggc cgacgaggct atcgaggcct gaccttcttc
      721 gatcctggca cgagcgacgg agctcgaccc cgccgggtag gcccgtcggg tcggccggtg
      781 gtcagtccgg ccggatgttg tggttgaacc ggaagacatt ggccgggtcg tggcgccgct
      841 tgaccgccac gaggcgttcc cagcgctctg cgccgtacgc ggccacgacc cgctcggctg
      901 actcgtcggc gggcgaatag ttgatgtagc cgccgcccga ctgcgacagt ggctcgagca
      961 ggtcggcgac gcggcgggcc cacccgatgg ccacctcgtc ctcgtcgggc gactcccaga
     1021 tcgacagcgc gctcgcgttc cagcgggctg cccgctggcc gaacgcagct ccacccgccg
     1081 gctcggtccg tcccgcaccg gtgatcccct cgatcaggac gaacgccttg ccgacggcgt
     1141 cgggggcact gacggccgca acgagcgcct cgaaggtcga cgtgtcgagc tcgcgcacaa
     1201 aatggccctt ccagtagtga cgcagcccga acggcaggat cccggcgagg gcctggaggt
     1261 ccaggtacgt ccgcgttccc aggtcatcgt cgacgacggg caagtcgcgc cgcaagagct
     1321 cgaggatggg ctcgggatcg ctgccgtcga cgatgccgag gaggaacgcg acgatcggcc
     1381 cgagatccgg gtcgcgatgg atcgtcaggg tcggcatggt ggccaacggg tagcgggcga
     1441 tcaaggtctc gacccgcgcc aggacctcgc cggcggcttc gggaaggtag cggatctcgc
     1501 cgccaaacat gggaccaacc ggcgtcagtc gatacgtgaa ccgggtgacg acgccgaagt
     1561 tgccgccgcc gccgcgcagc gcccagagga gctcctcgtc gccgccgggc ccggcgatca
     1621 cgaccgatcc gtcggccgtc acgacctccg cctcgatcag gttgtcgcag gtcaggccgg
     1681 cgaccggcat cagccagccg atcccgccgc caagcgtcag cccgccgacc ccggtgtcgc
     1741 cgaaggtccc gcccggcacg gcgaggccgt gggcgaaggc ggccgcgtcg atgtcgttcc
     1801 actgggcgcc gccgccgaca tgggcgattc gtcgctcggg atcgaccgtc acgtcccgca
     1861 tcgatccaag cgagacgacc agggcaccgt cggccacgcc gtggccggcg acgctgtggc
     1921 cgccgccacg gaccgcgatc gggagcccgg ccgtccgggc ggcccggacc gcgccgacga
     1981 cctcatcggt cgaggtgcag gggacgatga ccgccggccg ccgatcgagc atcccgttga
     2041 acgagatccg atgctcgtca taggccgggt gatcgggcag gatcgctgtc gccgggaacg
     2101 acgcggcggg cgcgatctgg tcgtcggtct gcatcggtcg cctccgtgct gatggatgcg
     2161 cagcgtacgc gccccggcgg acgagaaatt cgtcgggaaa acgtgcgcgt gctggggaac
     2221 acgtttacaa tggcgagacc atccgccgca acgcccggcg cgaaatcgtc gaacaggcga
     2281 ccattcgccc accaaccagc cgatcctgga gcgccccgcc ctcgtgacca cgccgatcct
     2341 gccgacgctg cagggacccg ccgatctccg ccgtctcacc gagctcgagc tcgaacagct
     2401 cgccggggaa gtccgcgacg cgatcatcgc gacggtcgcg acgaccggcg gccacctcgg
     2461 cagcagcctc ggcgtcgtcg aactgacgat cgcgctccat cgcctgctcg agtcgccgcg
     2521 cgaccggatc gtctgggaca ccggccacca ggcctatccc cacaagctgc tgaccggccg
     2581 ttttgcccgg ttcgggacgc tccgcgagct cggcggcatc ggcggcttcc cccgccgcag
     2641 cgagtcgccc cacgacgtgt tcgacggcgg ccatgccggg accgggctct cgatcgggca
     2701 ggggctggcg accgcccgcg acctgcatca cggcaacgag cggatcgcgg tcgtcgtcgg
     2761 cgatgccgcg atcacgagcg ggctgagcct cgaggccctc aacgacatcg gccagcggaa
     2821 gacccagctc ctgatcgtcc tcaacgacaa cgagatgtcg atcagcccga cggtcggcgg
     2881 tctctcgaag tacctgagcc agatcaagct gtcggggacg tggcagtcga gcaagaccgc
     2941 ctatgacgag ttcgtgggca ggatcccggc ggtcggcccg accgttcgtg agctcggcca
     3001 gcggctccgg gcctcggtcg tcaacttcgc ccagccgggc cgcctgttcg aggacctcgg
     3061 gatcacctac atcggccttg tcccgggcca cgacctcaag gcgctgctct cgacgttcca
     3121 gcaggcgctc gagctcaagg gcccggtcat cgtccacgtc cggacccaga agggccacgg
     3181 cttccggccg gccgaggccg accaggtcgg cttccacggc gctgccctgc cgccgatcgc
     3241 cgtccccgcg tcgacgaacg gccatgtcga cggcaatggg gcgacgccgc tggcggccgt
     3301 gagtgccgtt cccgggctcg gttcgatgcc gacggagtcg atggccgacg acgcggcgcc
     3361 ggcatcggcc atcatccgac ccgcgaaaaa ccccaactac accgcggtct tcgccgcgga
     3421 gctgatcgag cgggcccgcg ccgacgagcg gatcgtcgcg atcactgccg ggatgccgac
     3481 cgggaccgga ctctcgatgt tcgcgaccga attcccggac cggttcatcg atgtcggcat
     3541 cgccgagcag cattcggtcg cgctcgcgac gggtctcgcg atgggcggga tgcgcccggt
     3601 ggtcgcgctc tactcgacgt tcctccagcg ggcattcgac cagacggtcc acgacgtctg
     3661 ccagaacgac cagccggtcc tgatcgcggt cgatcgggcc gggctcgtcg gggaggacgg
     3721 gacgagccac caggggatgt tcaccctgac cgcccagcgc cagctaccga acctcgtgat
     3781 cgccagcccg aaggacgagc aggagctgcg ctcgctcctc gtgagcgcgc tcggccagga
     3841 ccggccgttc gccctgcatt acccgcgtga cccgggcttc ggcctggcgc cggtcgagcc
     3901 ggctgcgatc ccgatcggcc gtggcgaaat gctgcgcgag ggcacggacc tcctgttcgt
     3961 cgggttcggc ccgatcgtgg cccgggcggt cgcggccgcc gatgccctcg ccgccgaggg
     4021 ctggtcggtc gcggtggtca atgcccgctt cgccaagccg ctcgaccgcg agctgatcct
     4081 cggccaggct cgcgacaaga agatcatcgt gaccttcgag gaaagcgtcg tcaccggcgg
     4141 gttcggcagc ggcgtgctcg aggccgtcga ggaggcgcgg ctcgtcgaac ctgccctgcg
     4201 cgaggtgcct gtgaagatcc tcgggatccc gggcgggtcg ttcgtcgatc acggctcggt
     4261 cgccgacctg cggcgggtgc tccggctcga tgcgaacggg ctggcggccc aggtccgcga
     4321 gacgctcgac cagctcggcg cccgcccggc gagcggcgcg gtggttggca tggggcatcg
     4381 atagctcgcc accgggcatc ggtagtcgaa gacggcccga cgccttcgac gatcgcccag
     4441 ggacgcggcg gacaccgccc gatccgtgtc atccaccatg gccggtcgtg gctgtctggc
     4501 gaggcggacc ggagcctggt ccccaccacc ccggatggtg aacgacaggg cccagggcgg
     4561 actggcgcgt ctagctcgac ggagggccgg ccaccgcgcg ccgggtgtcc ggggccacga
     4621 ggcagacgaa cgtggtgacc cagactgcca cgccgaaccc gaggatgagg tcgggaccgg
     4681 cggccagctg cgcccccgtc agcaggacca gcccgaccac gctgagcagg agtccgaggc
     4741 gccacgccca acggcggaag atcaggagcc cgactccggc gaggaccgct gcgaagccgt
     4801 agacggccat cgtcgtgccg aaggccaggg cggccgcccg gacgtccgcc gtcgtgctcg
     4861 agccgttcgc gatcgtcgag aggagaccgc cgccggcggc catgcccgat agtccgatca
     4921 ggatcaggag gccggcggcg aggatcaggc tgatcggtcg ggcgagcatc ggacggaacg
     4981 ctccgtggca attgggctcg gtcggcgctg ccggtcgtgt tggtgagcca tcgtgcaggc
     5041 ggctgtgcca cgatagccgg gtgagcccga ccaccgttcg tcgccgccgc cagcgcctcg
     5101 accagctcgt cgtcgagcgc ggcctcgcgg acagccgcgc ccgggcccag gcgctgatcc
     5161 tcggcggcaa ggtccggacc ggcgatggcg acggcgcgcg tctcgatcgg aaggccggcg
     5221 acctcgtcga cccggacgtg acgatcagcc tgatcgagac ggatccgtac gtgagtcgcg
     5281 gcgggcacaa gctggtggcc gccctcgatt cgttcgggat cgacccggcg gggctcgtct
     5341 gcctcgacgc cggggcgtcg accggcggct tcagcgacgt gctgctccag cgcggcgccg
     5401 cccaggtcca tgcggtcgac gtcgggcggg gtcagctcgc cgatcgggtt gcccgcgatc
     5461 cgcgcgtcgt cgtccacgac cggatgaacg cccggacatt gaccgccgag acgctcggcg
     5521 agccgatcga gctcgcggtc atcgacgtct cgttcatctc gctggccctc gtcctggggc
     5581 cgatcgtctc gacgctgagg tcgcggccgc cggccgcgat catcgccctg gtcaagccgc
     5641 agttcgaggt cggcaagggt ggcacggacc atggcgtcgt ccgcgacccg gaggcccatc
     5701 ggaccgtcct ccagcgcgtg accgcggcgg cggccgggct cggtctgggg acccgcgcgg
     5761 tgatcgcctc gccgatcctg ggacccgagg gcaaccgcga gttcctcgtc catctccagg
     5821 ccggtccgag ttgcgcagag gtcggggagc ggatcgacga agccgtcgct ccgactgccg
     5881 cgggcgacgg gaccggaccg ccatgacgat ccagcggatc ggcttcgcct acaacccgac
     5941 caccgaggcg gccaacgagc tccgcgagcg ggcggagggc tggtgccgga tgcaccggat
     6001 cgagcactgg gcggcccagg ccgaggattt cgacctcctc tgccgcgagc tcgccacgac
     6061 cgacatcctg ctggtccttg gtggcgacgg gacgctcctc cgagcggcgc gggccgtgat
     6121 ccaggtcgat gtcccgctcc tcggggtgaa cctcggcaag gtcgggtttc tctcgaaggt
     6181 cgaggcgacc gacctggagg gcgtcctcgc caagctcgtc gcgggcgaat tcacgatcga
     6241 cccgcggatg gcgctcgagg cacggatcct gccggccggg cgggaggcgg atggcgagat
     6301 ccatcacgcc ctcaacgacg tcgtcatcgc ccgtggcgcg ctcgcccggg tgacccggct
     6361 cgacgtcgcg atcggcccga cccacctcgc gacgttcatc gccgacggcc tcatcgtcgc
     6421 cagcccgacc ggctcgaccg gctattcgtt cagcgccggg ggcccgatcg tcgccccgga
     6481 cagccgcaac ctgatcgtga ccccgatcgc ggcgtatctc tcggccgtgc gttcggtcgt
     6541 cgtcggaccc gatcaggtga tccgttgccg gatcgtcgac gcggccgagg cgatcgtctc
     6601 gatcgacggc cgcgaggacg tcgttgtcgc ggtcggcgac gtcgtcgagg tccgggcgct
     6661 ggtccgcccg atccgcctgg tcgcgccgag cggcgcgcag ccgttctggg acctgttccg
     6721 ccagaaggtc gagctcctgc cgtcgtgagg cttgcattcg cgtggcgatg acggacgagc
     6781 gggatcgttc cgctccgatg cgttccggcc cggccgagaa cgcggatcgg gggcgcgccg
     6841 tcgggcgcct gctcgagctg accgtgaccg acctcgccct gatcgagcgg ctgcggctcg
     6901 agctggcgcc cggcctgaac gtgttcaccg gcgaaaccgg ggcgggcaag agcctcctga
     6961 tcgatgccct gggcctggcg gtcggggcgc gcgcggattc gaccctcgtc cggcacggtg
     7021 cggagaccgc gcgggtcagc gccctgttcg atcgcctgcc cgagccgctg atcgcggcgc
     7081 gcgaggtctc cgtcgccgga cgctcgacgg cgcggctcga cgacgagccg gtcacggccg
     7141 cccggctcgc cgacgtcgcc ggcgccctcg tcgagatcca cggccagcac gatcagcagc
     7201 ggctcctcga cgaacgctgg cagcgcgacc tgctcgatgc gttcggcggg cacgccgtca
     7261 tgcgggcctc ggtcgcggct gccgtcgagc gctggcgcga gaaccgtgcg ctcctggcgg
     7321 ccctcgagct cgacccgcgc gagctggccc gacgcctggg attggccgag cacgaggccg
     7381 cggagatcgc ggcggcgcag ctccgacccg gcgaggccga cgagatccgg gtccgcctcg
     7441 aggcggccca gcacggcgag gcgatcgccc gcggcagcgc gaccctcgtc gacgccctgg
     7501 ccggcgagga acagggcgcc cgcgacgtcg ttgccgtcgc tctccacgag gtgcgcgccc
     7561 tcgcccggct cgaccagcgc ttcgtcgaga tcgccgagcg tctggccggc ctcgaggccg
     7621 agctcgacga cgtcgcttcg accgtccgcg acctcgctga ttcggtcgac cacgatccgg
     7681 ccgagctcgc ccgcctcgcc gaccggctct cgacgatcta cgggctcgag cgtcgcttcg
     7741 gcgacgacga ggaggcggtc atcgcccatg gcgagcgggc ggcggccgag gccgagcgcc
     7801 tgcgcggggt cgacgacgag cgggcggcgc gtcaggccga cgatgtccgg cttctcgagc
     7861 aggttgcggc ggtcgcggcc gacctgtccg cggcccgcgc gacggcaggt cgggccctcg
     7921 ccgaggatgt cggccgcgtc ctcgccgagc tcgggttccc gcccgacgtg ttcgaggtcg
     7981 ggctgggtcg acggccggcc gggcgcgccg aagccgcggt cgaaatcgat ggcgatgcgc
     8041 tcgccttcga cgccgcgggg atcgatcagg tcgtcttccg gatcgcgccg aatgccggcg
     8101 agccggcgcg atcgctggcg agaatcgcct cgggcggcga gctgagccgg gtcgccctcg
     8161 cgatcaagga agtcctcgcc gaggtcgacg acacgccgag tctcgtgttc gacgagatcg
     8221 atagcgggat cggcggccgc agcgcggacc cggtcgggcg gagcctgtgg agcctcggcc
     8281 ggcgccacca ggtcctgtgc gtgacccacc tcccgcagat cgcggctcac gccgacgcgc
     8341 acttccagat cacgaagcgg gagcgggacg ggcgcaccgt caccgaggtg acccgcctcg
     8401 accgcgaggg ccggatcgtc gagctgggcg agatgctggg cggggccgac gccgacggcg
     8461 cccagggatc gaccggtggc acggccacgc tggcgtcggc tcgcgagctg ctcgatcggg
     8521 cagaggcctg gcggatgcgg gccggggtgt ccgcctgagc ccggcagggc ggtctcgatg
     8581 cccgtgacgg ccgcgccgcc gctcgagcgc gcgatcgacg actacctcac gtacctgcgt
     8641 gtcgagcgcg gcctgtcgcc ggcgacgatc cgggcctatc gcggcgacct caccgacttc
     8701 gcggcggggg agggagcggc cacggcgtgg gccgcgggtc ccgaggccgc ccgacggcac
     8761 cttgcttccc ggacccgacg cggcgcgaag ccgccgcgcg accccggtct tgccccgacc
     8821 tcgctccgac gccgcgcggc gagcatccgc ggcttctatc gcttcgccta cggcgacggc
     8881 ctgatcgagc gcgatgtggc cgcccatctc gacctgccgc gccagccacg cctcctgccg
     8941 gagaccctca ctgtcgccga ggtcgaggcg ctcctcgatg cggccccgga tctccgggca
     9001 cgggcgctgc tcgagctgtt gtacgcggct ggcctgcggg tgagcgaggc gctcgggctc
     9061 gaccgggagg acctctcgac ggacggcggc ttcgtccggg tgatcggcaa gggcgacaag
     9121 gagcggctcg tgcccgtcgg cgacatcgcc ctcgactggc tcgagcgctg gatggcggac
     9181 ggtcggccgg ccctgctcga gctcgcccac gtcgaaccgc agcgcggcgg gccgctcttc
     9241 ctcggtgacc gcggtcggcg gcttgctcga cagcaggcgt tcacgatcgt caaggtcgcc
     9301 gcccgggcag ccggcctgtc ggacgccgtg agcccgcaca cgctgcgcca ctcgttcgcc
     9361 acccacctgc tcgaaggcgg tgccgacctg cggatcgtcc aggagttgct cggtcatgcg
     9421 agtatctcca cgacccagct gtacacccac gtgaccggtg agcgcgtccg cgaggtctac
     9481 gccaaagcgc atccccgagc ctgagaggga gaacgacacg ccatggccta tctcgacaac
     9541 ctgctcgcct cgggcgagca gccgctccgg gtcgaacacc agcactggtt cgtcgtcgcc
     9601 gccgacgcac gctatgcgtt cttcgcctgg atcggcgcga tcgcgctgct gatcgttgcc
     9661 cgcatgttgc ccgacttcat tccggacgtc ctcggctggg gcgtcgtcat cctcgtgatc
     9721 ggtggcctgc tgtacctcgg ctggcagatc ctgcgctggc agaacgagga gttcgtcgtc
     9781 acctcgcggc gcgttctcat gaccgagggc gtggtcaaca agcgggtgat cgacagctcc
     9841 ctcgagaaga tcaacgacgc gatcctgacc gagtcgatct tcggccggat gttcggcttc
     9901 ggcgacctcg agatcctgac cgcctcggag tcggggatct cacgcctccg gatgctccgc
     9961 aaggcggacg acttcaagcg gacgatgctc gacgccaagc acgagctcga gctcgagctg
    10021 tcgggcgccc gtccgatgcc gggcccagcg atccggacgg cggcaccgcc cgcaccgatg
    10081 cagcccacgg ccgcggtgcc ggcagccgcg cctccggccc ccgccgcggc cgcgctgtcc
    10141 gccgacgatg tgacccggac gctggccaac ctcgccgatc tccgcgatcg cggggcgatc
    10201 agcgccgagg agtacgaggc gaagaaggcc gacctgctgc gccgcctgta gcattcgagc
    10261 cagcggcgac ggccgcctgc ccggcagcac cgcgaaggat cgactcttgt tcgttcagca
    10321 acatccggtc gaagccctcg ccctcatcgc gttcttcctc ctgctggcat tcccggtcca
    10381 cgagttcgcc cacgcctatg cggcttaccg gctcggggac tcgaccgcga agctgttcgg
    10441 caagctgacc ctgaacccga tcgcccattt cgaccagatt ggcggatcga tgctcgtgat
    10501 ctcggtcctg ctgaccggct ttccgttcgg cttcgcccag acgcccgtca acccgcgcaa
    10561 cctgcggggc aagtacggcg aggtgatcgt cgcggcagcg ggaccgatct cgaacctgct
    10621 gatggccgca gccgtggcga tcccactccg gctcctgttc ccgctgacgg ccggcgatca
    10681 gcacatctac gtcactgaac cggagttggt cctcaagctc atcgtccagt acagcatcct
    10741 gctcggcctg ttcaacctga tcccgatccc gccgctggat ggcggcgcga tcatgctgtc
    10801 gctcgtctcg ccgcggaccg cgtggcaact ccgaccgatg ctcgcccaat acgggttctt
    10861 cctgatcatc ctgctgatca tcccgctcgg cggccagtcg attctgggcc gcgtcctgct
    10921 cccgatcgtc aatgcgatct atggcgtcct ggtgggctga gaaggtccgc caggtcggcg
    10981 cccacgtccg ggcgcgggtg agcgatgccg agcgccggca gctcgtgacc tggctgaccg
//
LOCUS       DATAIW010000476         9761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_229961, whole genome shotgun sequence.
ACCESSION   DATAIW010000476 DATAIW010000000
VERSION     DATAIW010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229961"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VHM48_14145"
     CDS             <1..448
                     /locus_tag="VHM48_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756605.1"
                     /translation="GPAPSSAGPDGPVLGKAVFEDGDGTHDAAVEPQPAGLSVDAADL
                     ELALRYVPDYRVLVLAADLDPPALDAVVAAAGWSGAHLVALIPDGASTPALPETTTVL
                     LRPPTDPDGAFASMVAAYAVALDQGSEPAAAFATAQQAGGWAAVAD"
     gene            complement(459..1208)
                     /locus_tag="VHM48_14150"
     CDS             complement(459..1208)
                     /locus_tag="VHM48_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX2756606.1"
                     /translation="MCGRFSQQRPASELAEIFAAEPLADDPGPRFNVAPTDDALVVVQ
                     RDDRRAITAYRWGLIPHWADAAKVGSRMFNARAETLTTSPAFRDAFRRKRCLVPVDGF
                     YEWHREGAHRQPFAIARDDGRPLALAGLWSGWRDPTADRVVRTFTIVTSRPNDQMAGL
                     HDRMPVIVPEDAWTRWLDPSLDDPAELNGLFEPSDEVGLRIWPVSPLVNNVRNDGPEL
                     FEPVVAGIEAGGPGHPDPQPGLGLFDEVVGS"
     gene            1248..2444
                     /locus_tag="VHM48_14155"
     CDS             1248..2444
                     /locus_tag="VHM48_14155"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2756607.1"
                     /translation="MDQPPDPTPAVDPLVPGPLDRSTAGQLLDRGAALLESGDYPEAL
                     ATYQRVVGFDDPEVTAAALLGMAQARYRMDDEDAAVRTWEAVLGLPETTSTYHAWRNV
                     AAARVRDGDLNGAIGAYREADRRAPAADKPEIANRLGWLAKETGNVRASRRYFARGRG
                     SVLPYVSFAIIGITVAVSVAAMLSTDIAQIYDVLQLDKRAVAAGEIWRLWTVTLLHDP
                     NNLLHLLFNMYALYLAGPIVERLYGSSLTAIFYLVCALGGSLGSFAFGSDAPSVGASG
                     AIFGLFGILLVATRTHNPALDRASRNFVTQLGTILMINIIFGIVAAGYIDNFAHIGGF
                     VTGALLGLAFVPGKVATIRSMWQAGAAGQLANGFIGSPIGRLAAVVALVGVMAVAAAF
                     GINRWG"
     gene            complement(2428..3912)
                     /locus_tag="VHM48_14160"
     CDS             complement(2428..3912)
                     /locus_tag="VHM48_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2756608.1"
                     /translation="MPASLTGRIVVAFGVLAVALWLAIGATMFVVLRGLHAEATSSAL
                     ADIVQTFAVRLRGAVADREVRTVVNQIRGEVAGGVTIHLLAADGHVVDVGLPDPAPSI
                     AIPIPPETRIGDTLTGTAPFADGQTHDYAALVLRGPNATAARAVLLSSVDHAGADALR
                     DVGRTLPIVILVTLAIGAPLAFLLSRSIARPLARLARRTSELVVPGAPVGPPLPVGGP
                     GEVRDATARVNVLAEELTRTRARESELLADLRHDLRTPLTVIAGYAAALADGTATGPA
                     AERAAAAIAEEAGRLERLVGELGAIDRLRAGTDALRLEPIDADAVLAATVDRFASAAR
                     TAGVTLEAGPAAAASAGETGVAGPIGSSLAADRIALDRILGNLVDNALAVVEPGGSIR
                     LEARRVHSVAGVDGPAIAFSVLDDGPGFAPGGTARAFERFYRGDPSRAGRGSGLGLSI
                     VRELALAHGGTAIAENLSPHGARVSVVLPVQPPERGRGAFSPSD"
     gene            complement(3920..4633)
                     /locus_tag="VHM48_14165"
     CDS             complement(3920..4633)
                     /locus_tag="VHM48_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756609.1"
                     /translation="MKTILVVDDERHIVDLVRLYLEKEGFAVVAAHDGVDALDRHARH
                     DPDLVILDLMLPKLDGFEVCREIRRRGDTPVLMLTARDDDVDAIVGLELGADDYVTKP
                     FNPRALVARVKAILRRTDVTARGGRPIEVGALRIDPRRREATVGDRNLGLRAREFDLL
                     AALARDPGVVLTRDALLEDVWGTDFPGETRTVDVHVSEVRRKLAPDGPPIETVRGVGY
                     RLVPPLRQPVSEPTAAPAD"
     gene            complement(4778..5335)
                     /locus_tag="VHM48_14170"
     CDS             complement(4778..5335)
                     /locus_tag="VHM48_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2756610.1"
                     /translation="MSIDPTDVLRPLRRTRQVREFEPTPVDPDILDALADVARWTGSS
                     KNSQPWRFIVITEPATIRAIHDAGLPQTRGLATAPAAIAIVLPAAPDRAVGHAYDEGR
                     AAERILVAARMLDIGAGISWIPAAVRPAIAALLDLPAGRMVRTIVQLGHPTEEARRPK
                     SAPGEARLPRQDIVFRERWPANDPD"
     gene            complement(5363..6913)
                     /locus_tag="VHM48_14175"
     CDS             complement(5363..6913)
                     /locus_tag="VHM48_14175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2756611.1"
                     /translation="MPNPGDLLMGRYRILERLGSGGMATVHRARDERLERDVAVKILL
                     PNLAGDPSTAARFEREARSLAASSHPGVVAVFDVDAGDPSTGREPFFVMELCRGGSLA
                     DRFGGGQRMAPDDLVPILVPVADGLADLHRRGVVHRDIKPQNILFGDDRAKLADFGLA
                     QSNDGQGPSELTTPGTAVGTLAYLAPEVLAGERATAAADVYGLGVVAFVGLTGQPPRP
                     VSSMAELVTSSGSAAPAVSTVAPDLGPAFDDIVGGALAVGPNDRPDALSFASGLAAAL
                     GRWTRDGGPARRSAAPVGPDAPLDAAFLVGVGRPPAGEPALADDATTAIAIPLGATAS
                     LPLEASGARGPARDSTRTGVARATSWVGRATVVAALVLAVMIALVSRIGSAPSSGPGA
                     STAASPAPLVSAPVPSSSPSIPPSPPAATPSPKLDPAFASLTAMDAAISAARGGPDGL
                     KGKEANDLEERTAAVRRALDAGDRSAALDAARKLDHRIADLAKDLGRDQATRLRTASQ
                     DLVRALGG"
     gene            6931..7869
                     /locus_tag="VHM48_14180"
     CDS             6931..7869
                     /locus_tag="VHM48_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756612.1"
                     /translation="MRYDRVPCLGDPMFSTLLGVLPADPDRPAAAGDEDAARLDDVAA
                     LAGVGLELISDGGDAAGPDADVAVVVARWQAAASVSAAAVKQVLLGPWSASRLDGGRP
                     EAAAERLRPTILALAAAGCPLVEIAEPDAIAIADDPDAALAFAAAHRQLVDGSERVHA
                     SLCLTGGNFDGAAPATFFDLAYASYAFDLIAGPENWRLIVQAPTDRGIVCGALGLGPG
                     ADETREVLVWAAHYAASTNGRGLARVGLANAPIGPGFQPPSRVDVLRKLALVAEASRI
                     AAVDSAEEMAGLLDTRAIGPRSARSARYAPARRRRS"
     gene            7971..8399
                     /locus_tag="VHM48_14185"
     CDS             7971..8399
                     /locus_tag="VHM48_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756613.1"
                     /translation="MANDLPGLARVVELRYVGRRTGRTRRTLVTLLSHDGTWYVGHPN
                     GEAGWVRNAEASGWVDVEPPGPLGPRFSVQRLDDGAERDAVIRSTARQQPFPANLLYR
                     AAQRHVAAVGVYHRLEPFRDAAPSPPPPIDPPAPSPEGAR"
     gene            8396..9655
                     /locus_tag="VHM48_14190"
     CDS             8396..9655
                     /locus_tag="VHM48_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2756614.1"
                     /translation="MTKSRFWHGFADMHTVAEAEVVIRSADGVWLEDTAGNRYLDATA
                     ALWYCNVGYGRRVIADAVAEQLARLPAYSSFGAYTSEPTLVLADRLAALAPIANAVVF
                     LGSGGSDAVDTAAKLVRRYWDVIGRPERRVIVSREFGYHGMHGWGTSLVGIAPLKSGY
                     GGNFIDEVVNVGAMDVETLGALFAERGHEIAAFIGEPVIGAGGVYPPEEHYWPEVNRL
                     CREHDILLVADEVITGFGRTGVMWGTQRYGIEPDLVTFAKGVSSGYVPLGGVLVGERV
                     RAPFWDKDVPGAVFRHGYTYSGHAGAAAAAMANLDILEGEGLVARVAELEPVLDREVR
                     RLESAPGVGEVRTVGLTAAVAFSPDLLAADAGLPERVVEAALRHGVATRVLRGHALQV
                     SPPFVITESEITTMVDGLGAALDEVAG"
BASE COUNT         1274 a         3492 c         3658 g         1337 t
ORIGIN      
        1 agggccggcc ccgtcgtcgg caggtccgga cggtcccgtg ctcgggaaag ccgtgttcga
       61 ggacggcgat ggcacccacg atgcggccgt cgagccgcag cccgccgggc tgtccgtcga
      121 cgccgccgat ctcgagctcg cgctccgcta cgtccccgac taccgcgtcc tcgtgctcgc
      181 ggcggatctc gatccgcccg ccctcgatgc ggtcgtggcc gccgcgggct ggtccggtgc
      241 ccacctggtt gcattgatcc ctgacggggc gtcgacgccg gcactccccg aaacgaccac
      301 cgtgctcctc cggccgccga ccgaccctga cggggccttc gcgtcgatgg tcgcggcgta
      361 tgcggtcgcg ctcgaccagg gcagcgagcc ggcggcggcg ttcgccacgg cccagcaagc
      421 cggaggctgg gcggcggtcg ccgactgacg ggccgggctc aggacccgac cacctcgtcg
      481 aacaggccca ggcccggctg cggatctgga tgaccggggc cgccggcctc gatcccggcc
      541 acgaccggct cgaacagctc cgggccgtcg ttgcggacgt tgttgacgag cgggctgacc
      601 ggccagatcc ggagcccgac ctcgtcgctc ggttcgaaca gcccgttgag ctccgccgga
      661 tcgtcgagcg acgggtccag ccagcgcgtc caggcgtcct cgggcacgat caccggcatc
      721 cgatcgtgga ggccggccat ctggtcgttc gggcggctcg tgacgatcgt gaacgtccgg
      781 acgacccgat cggccgtcgg atcgcgccag cccgaccaca ggccggcgag cgccagcggc
      841 cgcccgtcgt cgcgggcaat cgcgaacggc tggcggtgtg ctccctcgcg gtgccactcg
      901 tagaagccgt cgaccgggac gagacagcgc ttccggcgga aggcatcacg gaaggctggg
      961 ctcgtcgtca gcgtctcggc ccgagcgttg aacatccgcg agccgacctt cgccgcgtcg
     1021 gcccagtgcg ggatgaggcc ccagcgatac gcagtgatcg cccgccgatc gtcgcgctgg
     1081 acgacgacga gggcgtcatc ggtcggggcg acgttgaagc gcgggccggg atcgtccgcg
     1141 agcggctcgg ccgcgaagat ctcggcgagc tcggacgccg ggcgttgctg gctgaagcgt
     1201 ccgcacattc cagcaagtct agtggtcggt cctgtatcgt cggcccgatg gatcagccac
     1261 ccgacccgac cccggccgtc gatcccctcg tgccagggcc gctggatcgg tcgaccgcgg
     1321 gccagctgct cgaccggggc gctgccctcc tcgagagcgg cgactacccc gaggcactcg
     1381 cgacctacca gcgggtcgtc ggcttcgacg atccggaggt cacggccgcg gcgctcctcg
     1441 ggatggccca ggctcgctac cggatggacg acgaggatgc cgcggtgcgg acgtgggaag
     1501 cggtcctcgg gctgcccgag acgacctcga cataccacgc ctggcggaac gtcgccgcgg
     1561 cccgcgtccg cgatggagac ctcaacggcg cgatcggcgc ctaccgcgag gccgaccggc
     1621 gggccccggc ggccgacaag cccgagatcg ccaatcgcct cggctggctg gccaaggaga
     1681 cgggcaacgt ccgcgcgtcg cggcgctact tcgcccgcgg ccggggcagc gtcctgccgt
     1741 acgtgagctt cgccatcatc gggatcacgg ttgcggtctc ggtcgccgcg atgctgtcga
     1801 cggacatcgc ccagatctac gacgtcctcc agctcgacaa gagggcggtc gcggcggggg
     1861 agatctggcg cctctggacc gtgaccctcc tccacgaccc gaacaacctg ctccacctgc
     1921 tgttcaacat gtacgccctg tatctggccg gtcccatcgt cgagcgactg tacggctcga
     1981 gcctcaccgc gatcttctac ctggtctgcg cgctgggcgg atcgctcggc agcttcgcgt
     2041 tcggcagcga tgcgccgtcg gtcggtgcgt ccggtgcgat cttcggcctg ttcggcatcc
     2101 tccttgtcgc cacccggaca cacaacccag cgctcgaccg cgcgtcgcgg aacttcgtga
     2161 cgcaactcgg cacgatcctg atgatcaaca tcatcttcgg gatcgtggcg gccggctaca
     2221 tcgacaattt cgcccacatc ggcgggttcg tcacgggcgc actcctcggc ctcgccttcg
     2281 tgccgggcaa ggtcgcgacg atccgctcga tgtggcaggc gggggcggcc gggcagctcg
     2341 caaatggctt catcggcagc ccgatcggac ggctcgccgc cgtcgtggcg ctcgtcggcg
     2401 tgatggcagt cgcggccgcg ttcgggatca atcgctgggg ctgaacgctc cgcggccgcg
     2461 ttccggtggt tgcacaggta gcacgacgct gacccgggcg ccgtgcgggc tcaggttctc
     2521 cgcgatcgcg gtcccgccgt gggcgagcgc gagctcgcgg acgatcgaca ggccgaggcc
     2581 gctgccccgg cccgcccggg acgggtcgcc gcggtagaag cgctcgaacg ccctggccgt
     2641 gccaccgggg gcgaagcccg ggccatcgtc gaggacggaa aaggcgatgg ccggtccgtc
     2701 gacgccggcc accgaatgca cgcgccgggc ttcgaggcgg atcgacccgc ccggctcgac
     2761 gaccgccagc gcattgtcca cgaggttgcc gaggatccga tcgagggcga tccggtcggc
     2821 cgccagagac gagccgatcg ggccggcgac gccggtctcg cccgcgctcg cggccgcggc
     2881 cggccccgcc tcgagcgtca cgcccgccgt ccgtgccgcc gacgcgaacc gatcgaccgt
     2941 cgccgcaagc accgcatcgg cgtcgatcgg ttcgagccgc agggcgtccg tcccggcccg
     3001 taaccggtcg atcgcgccga gctcgccgac gagccgctcg agacgtccgg cctcctccgc
     3061 gatcgccgcc gccgcgcgct cggcggcggg accggtcgcc gttccgtcgg cgagggccgc
     3121 ggcgtacccc gcgatcaccg tcagcggcgt gcgcagatcg tggcgcaggt cggcgaggag
     3181 ctcgctctcg cgggcgcggg tccgggtgag ttcctcggcg aggacgttga cgcgggcggt
     3241 cgcgtcgcgg acctcgcccg gaccgccgac cggcagcggc ggaccgaccg gcgcgcccgg
     3301 caccaccagc tcgctcgtcc ggcgcgccag gcgggccagg ggccgggcga tcgagcggga
     3361 caggaggaac gccagcggag cgccgatcgc gagcgtcacg aggatgacga tcggcagcgt
     3421 ccggccgaca tcgcggagcg cgtcggcccc ggcgtgatcg accgacgaga gcaggaccgc
     3481 ccgcgccgcc gtcgcgttcg ggccgcggag gacgagggcg gcgtagtcgt gcgtctggcc
     3541 gtcggcgaac ggcgccgtgc cagtgagggt gtcgccgatc ctggtctcgg ggggaatcgg
     3601 gatcgcgatg ctgggcgccg ggtcgggcag accgacgtcg acgacgtggc cgtcggcagc
     3661 gagcaggtgg atcgtgacgc ctccggcgac ctcgccgcgg atctggttga cgacggtccg
     3721 gacctcgcgg tcggcgacgg cgccgcggag gcggacggcg aacgtctgga cgatgtcggc
     3781 gagggcgctc gacgtcgcct cggcgtggag cccgcgcagg acgacgaaca tcgtcgcccc
     3841 gatcgcgagc cacagggcga cggcgaggac cccgaacgcg accacgatcc gaccggtcag
     3901 gctggcgggc atcgtcgggt caatcggccg gcgcggcggt gggctcggaa accggctggc
     3961 ggagcggcgg gacgaggcga tagccgacgc cccgaacggt ctcgatcggc ggaccgtccg
     4021 gcgcgagctt gcggcgaacc tcgctgacgt ggacgtcgac ggtccgggtc tcgcccggga
     4081 agtccgtgcc ccagacatcc tcgagcaggg cgtcccgggt cagcacgacg ccggggtcgc
     4141 gggccagcgc ggcgagcagg tcgaactcgc gggcgcgaag gccgagattg cggtcgccga
     4201 ccgtggcctc gcggcggcgc gggtcgattc gcagggcgcc gacctcgatc gggcggccgc
     4261 cgcgggcggt cacgtcggtc cggcggagga tggccttgac ccgggcgacg agtgcccgcg
     4321 ggttgaaggg cttggtcacg tagtcgtcgg cgccgagctc caacccgacg atcgcgtcga
     4381 cgtcgtcgtc gcgcgcggtc agcatcagga ccggtgtgtc gccccgccgc cggatctccc
     4441 ggcagacctc gaacccgtcg agcttcggga gcatcaggtc gaggatcacg aggtcggggt
     4501 cgtgtcgggc gtgacggtcg agggcatcga caccatcatg ggcggcgacg accgcgaacc
     4561 cctccttctc gaggtacagc cggacgaggt cgacgatgtg ccgctcgtcg tcgacgacga
     4621 ggatcgtctt catcgggctc gcgtcgaggc gacgttcgtg cagccggcag ggcggatacg
     4681 catcgcggcg gaggatacgc ttcgtcccgg cgagcgatcc cctcgattgt caggcgaagg
     4741 taagccagcc atccgtgccg gcgctcatga ccggtcgtca gtccgggtca ttcgctggcc
     4801 agcgttcgcg gaagacgatg tcctggcgcg gcaggcgcgc ctcgcccggg gcggacttcg
     4861 gccggcgcgc ttcctcggtc ggatggccga gctggacgat cgtccggacc atccggccgg
     4921 cgggcaggtc gagcagggcc gcgatggccg gtcgcacggc ggccgggatc cagctgatcc
     4981 ccgcaccgat gtcgagcatc cgcgcggcga cgaggatccg ctcggcggcg cgaccctcgt
     5041 cgtaggcgtg acccaccgcc cgatcgggcg cggccggcag gacgatggcg atcgccgcag
     5101 gcgccgtcgc gaggccgcgc gtctgcggca ggccggcgtc gtggatggcg cggatcgtcg
     5161 ctggctccgt gatgacgatg aaccgccatg gctggctgtt cttgctgctg ccggtccagc
     5221 gcgcgacgtc ggcgagggcg tcgagaatgt caggatcgac cggagtcggc tcgaattcgc
     5281 ggacctggcg ggtccggcga agaggacgga gaacgtcggt cgggtcgatg ctcatcggaa
     5341 ccctcgatcg ggggtcatgc catcatccgc ccagtgcgcg gaccaggtcc tgcgatgcgg
     5401 tccgcaaacg ggttgcctgg tccctgccga gatccttggc aaggtccgcg atcctgtggt
     5461 cgagcttccg cgccgcgtcg agcgcggcgc tccgatcgcc ggcgtcgagc gccctgcgga
     5521 cggcggccgt tcgctcctcg aggtcgttgg cttccttgcc cttgaggccg tccggtccgc
     5581 cgcgggcggc gctgatcgcg gcgtccatcg cggtcaggct ggcgaacgcc gggtccagct
     5641 tcggagacgg cgtcgcggcc ggcggggacg gcgggatcga cggcgatgac gacggcacgg
     5701 gcgcgctcac gaggggcgca ggcgatgcgg cggtggaggc gccgggcccg gacgacgggg
     5761 cggaaccgat ccggctgacg agcgcgatca tgaccgcgag gacgagggcg gccacgacgg
     5821 tcgcccgtcc gacccaggac gtggcccgag cgaccccggt ccgggtcgag tcccgggcgg
     5881 gccctcgggc gcccgaggcc tcgagcggga gagacgcggt cgcgccgagc gggatcgcga
     5941 tcgccgtggt cgcatcgtcg gccagcgcgg gttcgcccgc cggcgggcgc ccgaccccga
     6001 cgaggaacgc agcgtcaagc ggcgcgtcgg gtccgaccgg tgccgcggat cggcgcgccg
     6061 gcccgccatc gcgcgtccag cgcccgagcg cggcggccag cccggacgcg aacgagagcg
     6121 cgtcgggccg gtcgttcgga cccacggcga gcgcgccgcc gacgatgtcg tcgaaggcgg
     6181 gccccaggtc aggcgccacg gtcgagaccg cgggcgcggc cgatcccgac gaggtgacga
     6241 gctcggccat cgaggacacg ggccggggcg gttggccggt cagcccgacg aaggcgacga
     6301 cgccaaggcc gtagacgtcg gcggccgccg ttgcccgctc tccggcgagg acctcgggtg
     6361 cgagataggc cagcgttccg acggccgtcc cgggcgtcgt gagctcgctc ggaccctggc
     6421 cgtcgttcga ttgggccaga ccgaagtcgg ccagcttcgc ccgatcgtca ccgaacagga
     6481 tgttctgggg cttgatgtcg cgatggacga cgccccggcg atggagatcg gccaggccgt
     6541 cggcgaccgg gacgaggatc gggaccaggt cgtccggggc catccgctga ccaccgccga
     6601 aacggtccgc gagggacccg ccgcggcaga gctccatgac gaagaagggc tcgcgaccgg
     6661 tggacggatc gcccgcgtcg acgtcgaaga cggccacgac gccggggtgg gaggacgcgg
     6721 cgagcgagcg tgcctcgcgc tcgaaccggg ccgcggtcga cgggtcgccg gcgaggttgg
     6781 gcaggaggat cttgaccgcg acgtcgcgct cgagccgctc gtcgcgggct cgatggacgg
     6841 tcgccatgcc gccggacccg aggcgctcga ggatccggta gcggcccatg aggaggtcac
     6901 ccgggttggg catctcggcg agcctacccg atgcgctacg atcgggtgcc ctgcctcgga
     6961 gacccgatgt tctcgacgct gctcggtgtg ctcccagcgg acccggaccg accggccgcg
     7021 gccggcgacg aggacgcggc gcggctcgac gatgtcgcag ccctggctgg tgtcgggctc
     7081 gagctgatct cggacggcgg cgacgcggcc ggtccggatg ccgatgtcgc ggtcgtcgtg
     7141 gcgcgctggc aggcggcggc ctcggtcagc gccgccgcgg tgaagcaggt cctgctcgga
     7201 ccgtggtcgg cgtcgcgtct ggatggcggg cgaccggagg ccgcggccga acggcttcgg
     7261 ccgacgatcc tcgccctcgc cgccgcgggc tgtccgctcg tcgagatcgc cgagccggat
     7321 gcgatcgcga tcgcggacga tcccgacgcc gctttggcct ttgccgctgc gcaccggcag
     7381 ctcgtcgacg gcagcgagcg cgttcacgcc tcgctgtgcc tgaccggtgg caacttcgac
     7441 ggcgcggccc ctgcgacgtt cttcgacctc gcctacgcca gctacgcctt cgacctgatc
     7501 gccggtccgg agaactggcg gctgatcgtc caggcaccga ccgaccgcgg catcgtctgc
     7561 ggcgccctcg gcctgggtcc cggcgcggac gagacccgtg aggtcctcgt ctgggccgcc
     7621 cactacgcgg cgtcgacgaa cggccgcggc ctcgcccggg tcgggctggc gaacgccccg
     7681 atcgggcccg gtttccagcc gccgagccgg gtcgacgtgc tgcgcaagct tgcgctcgtc
     7741 gccgaggcga gccggatcgc cgccgtcgat tcggccgagg agatggccgg actgctcgat
     7801 acgcgggcga tcggcccgcg ctcggcgagg tccgcccgat acgcgccggc gcgacgtcgg
     7861 cggtcgtgac gcctccgccg ctgcgtcctc gccggtcggt cctgtggtcg gtcgcctggc
     7921 gcgccggtta ccgattcgtg cgcgtcctcg acccgttgat ccgatcgtgg atggcgaacg
     7981 acctgcccgg cctggcgcgc gtcgtcgagc tccgctacgt cggccgccgg acgggccgga
     8041 cgcgccgaac gctcgtgacg ctgctcagcc acgacggcac gtggtacgtc ggccacccga
     8101 atggcgaggc gggctgggtc cgcaatgcgg aagcgtccgg ctgggtcgat gtcgagcctc
     8161 ccgggccgct cgggccgcgc ttcagcgtcc agcggctgga cgacggcgcc gagcgggacg
     8221 ccgtcatccg gtccaccgcc aggcagcagc cgtttccggc gaatctcctc taccgcgcgg
     8281 cccagcgcca tgttgccgcc gtcggcgtct accatcgact cgaacccttc cgtgacgccg
     8341 cgccgtcgcc gcctccgccg atcgatccac ccgccccgag ccccgaagga gcccgatgac
     8401 caagagtcgt ttctggcacg gattcgccga catgcacacc gtcgccgagg cggaggtcgt
     8461 catccgctcc gccgacggcg tctggctcga ggacaccgcg ggcaaccgct atctcgacgc
     8521 gaccgcggcc ctctggtact gcaacgtcgg ctacggccgg cgggtgatcg ccgacgcggt
     8581 cgccgagcag ctcgcccggc tgcccgccta ctcgtcgttc ggcgcctaca cgtcggagcc
     8641 gacgctggtc ctcgccgatc ggctggccgc cctcgccccg atcgcgaacg cggtggtctt
     8701 cctcggatcg ggcggctcgg acgcggtcga cacggccgcc aagctcgtcc gccgctactg
     8761 ggacgtgatc ggccggcccg agaggcgggt catcgtctcg cgcgagttcg ggtatcacgg
     8821 aatgcacggc tgggggacgt cgctggtcgg gatcgccccg ctgaagtccg gctacggcgg
     8881 caacttcatc gacgaggtcg tcaatgtcgg ggcgatggac gtcgagacgc tcggcgcgct
     8941 cttcgccgaa cgcggccacg agatcgcggc gttcatcggc gagccggtca tcggcgccgg
     9001 cggcgtctac ccgccggagg agcactactg gccggaggtc aatcggctct gccgcgagca
     9061 cgacatcctg ctcgtcgcgg acgaggtgat caccggcttc gggcggaccg gggtcatgtg
     9121 ggggacccag cgctacggga ttgagccgga cctcgtgacg ttcgccaagg gcgtgtcgtc
     9181 cggctacgtg ccgctcggcg gcgtgctcgt cggcgagcgg gtccgggcgc cgttctggga
     9241 caaggacgtg cccggcgccg tgttccgcca tgggtacacg tactcgggtc acgccggcgc
     9301 ggcggccgcg gcgatggcca acctcgacat cctcgagggc gagggcctcg tcgcccgggt
     9361 cgccgagctg gagcccgtgc tggaccgcga ggtccgccgg ctcgagagcg cgcccggggt
     9421 gggggaggtg cggacggtcg ggctgaccgc cgccgtcgcg ttctcgcccg atctcctcgc
     9481 cgccgacgcc ggcctgcccg agcgtgtggt cgaggcggcg ctccggcacg gggtggcgac
     9541 gcgggtcctg cgcggccacg ccctccaggt gtcgccgccg ttcgtgatca ccgagtcgga
     9601 gatcacgacg atggtcgacg gcctgggcgc cgccctcgac gaggtggcgg gatagcgccg
     9661 ctcctcgtcg agcgctgact cagtcgccga gtcaggacgg ccaccagcac gaggaggtcc
     9721 gctgtggatt ccgcactgtc gagcgtgcta cccgtcgact t
//
LOCUS       DATAIW010000477         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_256418, whole genome shotgun sequence.
ACCESSION   DATAIW010000477 DATAIW010000000
VERSION     DATAIW010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256418"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..2952
                     /locus_tag="VHM48_14195"
     CDS             577..2952
                     /locus_tag="VHM48_14195"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX2756615.1"
                     /translation="MPTIAAADPRPSWGDAIADLEDKLTGRVLAVVGGVALVAGAIFF
                     LSLAFSRDWIGHEGRVLIGIGSAGFALAVGAWELDHRQPTVGRVLVAVGLAVVSLSMF
                     AATRLYNLVPVEIALLGSLVAAVAAAFLAVRNDAQIIAVLSLLAVLGAPPVLGASPTL
                     VTVAYIGTVLVGFAALALFRTWRWLPSIAFALSAPQLASWLAGDIVPAVGIVAAVVFW
                     TLNVVTAGGEEYLRPRNQLRLGSATLLLANAAFLLWAGFTLLDGPNEFLRGPFLIAIA
                     LAHAVLGGWFLLREGDHHPFGLLAAGTGVAALAMAAPVQAGGPPVPIAWAAEAAALAW
                     LASRRAHPQAALAAAILGLMALAHLVGFEYPAGMASRVPTAGDWPFVNASGLTLVFLL
                     GAATVAAWFLRARAVVAVIAGFGVLAVALAAPHELTGPALIAAWVGLALGALALERFI
                     IDRLPEEGPVTVGLLGELRFATLAAAASVGAIALGHALVFDLPIDAFGTIRPPAIPFS
                     DARALTLEFLVIGSAISGWVYLGRVTLELPAVAALVGAGLILAYGAVFEVYADAAIVI
                     AAGLAVAGLLLGTLRGVAERAWRLATGASLALVGVGTLAALSVIAGLDRLVMAEGIFI
                     DHPPFVSDVSAAYGALAVALAVAVRLNRRQGFAPWAGIAAGGMVVFALSVGVIDLFQP
                     RTGGAIQFEELARQAQVVMSVLWIVLGAAVFVYGLARGHLVVRQAGLGLLALASLKVF
                     VFDLASLDVAYRVLSLVALGTFLLLTAWVYARLRPPPTKPPRSSGPEAAAT"
     gene            3021..>3798
                     /locus_tag="VHM48_14200"
     CDS             3021..>3798
                     /locus_tag="VHM48_14200"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756616.1"
                     /translation="MRKTGPILILIIGILALIIDFVPGLSAPWEPDRVFETKVGLDLQ
                     GGLRVEYQAQPVEGRSAGQGDLDVIRTIIENRVNSTGVAEPVITVQGNDRIVVELPGV
                     SDPNAIRNLVGQTGSLDFVPLGKTSKAKGDTIDSNQFPALFGGTELSSATIGQNQTLG
                     RVVTFQLKDTGAKLFGEYTAQHIGDFFAIVLDHRVISAPTIDSAIPGGSVEISQKGTI
                     GGYPLKEATELVTVLQYGSLPFPIKELSSQTISATLGEQFL"
BASE COUNT          439 a         1402 c         1306 g          651 t
ORIGIN      
        1 cggcgcgccc tgctgcgcgt gttcgggtcg gcaaagcggg tgcgcgaggc gccggtcgag
       61 cagatcgccg cggttccggg gatcggcgcc gcgctggctg cccggatcaa ggagacgctg
      121 gaagcctgag cctggcccga caccggatcg gttgtcggat ggcggacagg ctcgttgcgc
      181 catcggcggc gcacgattca gagcggcaga cacggcgggc caccggtctg caacgcgtcc
      241 cgcactcggg cgtcatctca gtgcgtacgg cgacgcgaat ccgcaccagc gaggctccac
      301 gacatggacg aggacgagca ggttgacgcc cggtttcggg cgctcgaggc acggatcgcg
      361 agcctggaag cggccactgg cgcggcgccc gcgcaggcgc ggcccacggt cgcggcccgg
      421 ccggcgacga cggcaggact ccgcatcccc gatctgcagc gagcgtcccc gatcgtgagc
      481 gcgtcggtca cacccgccgc cgcgctccca gccgctggcg tggccctgcc tggcgcgagg
      541 gtttccaccc cggcaccgac ggccccgccg aggccgatgc cgaccatcgc cgcggcggac
      601 ccgcggccct cgtggggcga cgcgatcgcc gacctcgaag acaagctgac cggtcgcgtc
      661 ctcgccgtcg tcggcggcgt ggccctcgtc gcgggcgcga tcttcttcct cagccttgcc
      721 ttcagtcgag actggatcgg tcacgagggc cgggtcctga tcgggatcgg gtcggccggg
      781 ttcgctctgg cggtgggtgc ctgggagctc gatcatcggc agccgacggt cggccgggtc
      841 ctcgtcgcgg tcggcctcgc cgtcgtctcg ctatcgatgt tcgcggcgac gcgtctgtac
      901 aacctggtcc cagtcgagat cgcgttgctc ggctcgctcg tggccgcggt cgccgctgcc
      961 ttcctcgcgg ttcgcaacga cgcccagatc atcgccgtcc tgtcgctcct cgccgtcctc
     1021 ggcgctccgc cggtcctcgg cgcgtcgccg accctcgtga cggtcgcgta catcgggacg
     1081 gtcctcgtcg ggttcgcagc gttggccctg ttccggacct ggcgctggct gccgtcgatc
     1141 gccttcgcgc tgtccgcgcc acagctcgca tcctggctcg ccggcgacat cgtgccggcc
     1201 gtgggcatcg tggccgcggt cgtgttctgg accctcaatg tcgtcaccgc cggtggggag
     1261 gaatacctgc gcccgaggaa tcagctgcgc ctcggcagtg cgaccctcct cctggcaaac
     1321 gcggccttcc tcctgtgggc cgggttcacg ctcctcgacg gtcccaacga gttcctgcgt
     1381 ggtccgttcc tcatcgcgat tgcgctggct cacgcggtgt tgggcggctg gttcctgttg
     1441 cgcgagggcg accaccaccc cttcggactt ctcgcggcag gcaccggcgt cgcggcgctg
     1501 gcgatggcgg cgcccgtcca ggccggcggc ccccccgtcc cgatcgcgtg ggcggccgag
     1561 gcggcggccc tggcctggct ggccagtcgc cgagcccatc cccaggccgc cctcgccgcg
     1621 gcgatcctcg ggctcatggc cctcgcccat ctcgtcgggt tcgaataccc ggccgggatg
     1681 gcatcacgtg tcccgaccgc cggcgactgg ccgttcgtca acgcgagcgg gctcactctc
     1741 gtcttccttc ttggcgctgc aaccgtcgcg gcctggttcc tccgggcccg cgcggtcgtc
     1801 gcggtcatcg ccggtttcgg cgtgctggcc gtggccctcg ccgcccccca cgagctcacc
     1861 ggtccggccc tgatcgcggc ctgggtcggg ctcgccctcg gagcgctggc gctcgagcgg
     1921 ttcatcatcg atcggttgcc cgaggagggt ccggtcacgg tcggcctgct cggtgagctg
     1981 cgcttcgcca cccttgccgc agcggcgtcc gtcggggcga tcgctctcgg tcacgccctc
     2041 gtgttcgacc tcccgatcga cgcattcggg acgatccggc cgcccgccat cccgttcagc
     2101 gacgcccggg cgctgacgct cgaattcctc gtcatcggca gcgccatcag tggctgggtc
     2161 taccttggcc gggtgaccct ggagctgccg gccgtcgccg ccctcgtcgg cgccggattg
     2221 atcctcgcct acggcgccgt cttcgaggtc tacgcggatg ccgcgatcgt catcgccgcc
     2281 ggcctcgcgg tggccgggct cctcctcggg acgctgcgtg gcgtcgccga gcgggcatgg
     2341 cggctcgcca cgggcgcgag cctggccctg gtcggggtcg ggaccctggc cgcgctgtcg
     2401 gtcatcgctg gcctggaccg cctcgtgatg gccgagggca tcttcatcga ccaccctccg
     2461 ttcgtcagtg acgtatccgc ggcgtacggt gcgctcgcgg tcgccctcgc ggtcgcggtg
     2521 cggctcaacc gccggcaagg cttcgcgcca tgggcaggga tcgcggccgg cggcatggtc
     2581 gttttcgccc tgtcggtcgg cgtgatcgat ctcttccagc cgcggacagg cggtgcgatc
     2641 cagttcgagg agctcgcccg gcaggcccag gtcgtcatga gcgtgctgtg gatcgttctc
     2701 ggcgctgccg tgttcgtcta cgggctcgcc cgtggacacc tggtcgtccg ccaggctggt
     2761 ctcgggctcc tcgccctggc gtcgttgaag gtcttcgtct tcgacctggc ctcgctcgac
     2821 gtcgcctacc gggtcctgtc cctcgtcgcc ctcgggacgt tcctgctcct cacggcctgg
     2881 gtctacgccc gccttcggcc gccgccgacg aaaccgccgc gctcgtcggg gccggaggcc
     2941 gccgcgacct gaccgcgggg gacacgtcgc cgcgaccgat cggcggtctc ggggtccctt
     3001 tcctgtatca tccgccgctg atgcgcaaga ccggtccgat cctcatcctg atcatcggga
     3061 tcctcgccct gatcatcgat ttcgtcccgg gcctgtccgc gccctgggag cccgaccgag
     3121 tcttcgagac gaaggtcggg ctcgacctgc agggcggcct ccgcgtcgag taccaggccc
     3181 agcccgtcga gggcaggtcg gccgggcagg gggatctcga cgtcatccgg acgatcatcg
     3241 agaaccgggt caactcgacc ggcgtcgcgg aaccggtcat caccgtccag ggcaacgacc
     3301 ggatcgtcgt cgagctgccg ggcgtgtccg acccgaatgc gattcgcaac ctcgtcgggc
     3361 agacgggcag cctcgatttc gtcccgctcg ggaagacgtc gaaggccaag ggcgacacga
     3421 tcgattcgaa ccagttcccg gcgctcttcg gcggcacgga gctgtcgtcc gcgacgatcg
     3481 gccagaacca gaccctcggg cgggtcgtga cgttccagct gaaggacacg ggcgcgaagc
     3541 tgttcgggga gtacaccgcc cagcacatcg gcgacttctt cgcgatcgtc ctcgaccaca
     3601 gggtgatctc ggccccgacc atcgattcgg cgatccccgg tggcagcgtc gagatctcgc
     3661 agaagggcac gatcggcggg tatcccctca aggaggccac ggagctggtc acggtcctcc
     3721 agtacggctc gctgccgttc ccgatcaagg agctgtcgtc ccagacgatc agcgcgaccc
     3781 tcggcgagca gttcctga
//
LOCUS       DATAIW010000478         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_137425, whole genome shotgun sequence.
ACCESSION   DATAIW010000478 DATAIW010000000
VERSION     DATAIW010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137425"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VHM48_14205"
     CDS             <1..901
                     /locus_tag="VHM48_14205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX2756617.1"
                     /translation="EPRWNAMPVLEDVRDPSLIHWASFGKPWEPELTYGQDRWLEYAA
                     RLHDRAGAPPTADEGTSGRVGSIRNPDSIGPATGELSAAIEGVIEAVRSEHLSYLDAS
                     SLRTLAATVESIEADGIDGLIIETGTARGGSAITMAAAKSTTRRMKVYDVFGMIPPPG
                     EKDGDDVHRRYATIASGASKGVGGETYYGYREDLLAEVRESFGRHGVPIGDHNVELIQ
                     GLFEDTLTLDEPVALAHLDGDWYASTMTCLTRIAPLLVVGGRFVIDDYDTWSGCRAAV
                     DEYFARRDGFRFERRGRLHIVRV"
     gene            1063..2115
                     /locus_tag="VHM48_14210"
     CDS             1063..2115
                     /locus_tag="VHM48_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2756618.1"
                     /translation="MLDRFFGIFSRDIGIDLGTANTLVHVRDRGIVMSEPSVVAIDAK
                     TKRVLAVGAEAKRMVGRTPASIIAVRPLRDGVISDFDVTEQMIKYFVDKVHDRIGLIA
                     RPRMLLGIPSGVTEVEKRAVRDAAMNAGARWARLIEEPMAAAIGAGLPVSEPSGSLIV
                     DIGGGTTEVAVISLGGIVVSRSIRIGGDEMDQDIVAFARREYNLFLGERTAEDIKIAI
                     ASAYPGDWDSQRVSLRGRDLLTGLPRSVEVGADQIREAIEPSVVQIVDTIKDTIEETP
                     PELVADIMDQGIVLAGGGALLSGLDRRVADATQMPVHVADDPLTCVARGTGVVLQELE
                     KMDRVLVSDTYARAPR"
     gene            2168..3139
                     /gene="mreC"
                     /locus_tag="VHM48_14215"
     CDS             2168..3139
                     /gene="mreC"
                     /locus_tag="VHM48_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX2756619.1"
                     /translation="MTTILANRATRRRAVTFTILLAVSLILMAISSSPAVTDFQGAMA
                     YALRPIQGAIHGVADSVANAVAAVGEIQGLHSDNAALRRENQRLTEENLRATAIEQEN
                     EQLTGLLQLRNSYKYETAAAEVIARESSEARRSVTISKGTDDGIATGDVVIAEGGALV
                     GRVSDVGPNFAIVTLITDRSSIVIGETETSTASGEVVGQLGGALIMQNIDSTEKVVPG
                     EQVLTPGTEVAGGIRSPYPKGLVIGEVSDIRKDANSVVQMAYLLPTTDLDKVAYVLVI
                     LDYAGGLPGVDDTPIDCSQTGVGGALPGGEQPCIQPSASPRATPLLP"
     gene            3144..3905
                     /locus_tag="VHM48_14220"
     CDS             3144..3905
                     /locus_tag="VHM48_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2756620.1"
                     /translation="MKGIVLAGGTATRLFPLTIVTNKHLLPIYDRPMIYYPIETLAGM
                     GIREVMVIVGGKSVGDVVELLADGSHFGLDLTYRYQHGALGIAHAIGLARDFVGDDSF
                     CCILGDNILRGPALAATAAEFDSGPWGAGTLLYRVADPERFGVAELDTDGRVIGFEEK
                     PAHPKSDLIPIGVYFLRPDAFSVIDTLAPSGRGEFEITDVLNHYIPDGRLFSRVYDGL
                     WADAGTVPSLIRAAELARDDDDAGRLAPPVERPIG"
     gene            3913..5037
                     /gene="rfbB"
                     /locus_tag="VHM48_14225"
     CDS             3913..5037
                     /gene="rfbB"
                     /locus_tag="VHM48_14225"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX2756621.1"
                     /translation="MTLEGGSARIAPGARILVTGGAGFIGSAYVRDVLGRGDATRITV
                     LDKLTYAGNRANLAAVEADPGQAARYRFVQGDIAEPGIVGPLVAEVDAVVNFAAESHV
                     DRSILDPEAFLRTGVIGVHVLLEAVRAASTARAAAGGSPVRYLQVSTDEVYGSVAEGR
                     SVETDRLDPRSPYSAAKAAGELLVGSYVVTHGVDAVVTRGSNTYGPFHHPEKLIPLFV
                     TNAIDDQPLPMYGDGLQQRDWLFVSDHAGAIDHVLRRGASGETYNLPGSAEMTNRAVI
                     AALLERLGKPWSLVRTVPDRPGHDRRYALDGTKLAALGWRNRTKFADGLAATIDWFRA
                     NEAWWRAAKSGDWDAYYERQYGARLASGQAVAQVSAPTEA"
     gene            5040..5963
                     /locus_tag="VHM48_14230"
     CDS             5040..5963
                     /locus_tag="VHM48_14230"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2756622.1"
                     /translation="MRVAVTGATGRLGRAVVTALEEAPFTGPFGPIAWSRAIFDLDAP
                     DSAGALFDRDRPEVVVHCAAWTDVDGCARDPGLALARNGTATDVLARAAAERGVDLIV
                     VSTNEVFDGRRTDRLGYGPDDAPSPINPYGASKLAGEVAAGDAFAAASGGAALGIART
                     AWLYGPPGNDFPARILAAAERATATGEPLRLVADEIGSPSYTLDVADAIAELIGAAAV
                     EGTHHLVNGGTASRADWARELLRQAGVAVVTEDVPAATWPRASTPPAWAVLEPTPLPG
                     GEPLRPWQAALADYLPTLLRDRARAKAGAGR"
     gene            5960..6562
                     /gene="rfbC"
                     /locus_tag="VHM48_14235"
     CDS             5960..6562
                     /gene="rfbC"
                     /locus_tag="VHM48_14235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX2756623.1"
                     /translation="MSDPRPSLERSPSALPGVRYGAIARHADSRGAFRELWRASSFPI
                     LTPAGTGAPAGTEPRFVQANQSSSAAGVLRGLHYHRHQLDYWVVGSGRAFVALVDVRR
                     VVGGVGPAVVETRELDAGEWVVIPAGVAHGFLALEPLELVYFVTNEFDGSDELGFAWD
                     DPAVAVPWPAVDATPDDRPILSERDQSNPPLAELVASLRG"
BASE COUNT          983 a         2437 c         2361 g          992 t
ORIGIN      
        1 cgagccgcgc tggaacgcga tgccggtcct cgaggatgtc cgcgacccgt ccctcatcca
       61 ctgggcgagc tttggcaagc cctgggagcc ggagctgacc tacggccagg accgctggct
      121 cgagtacgcc gcgcgactgc atgaccgggc cggggcgccg cccaccgccg acgaaggaac
      181 gtcaggtcga gtgggatcca tcaggaaccc ggactcgatc ggtccggcca cgggagagct
      241 gtcggcggcg atcgaaggcg tcatcgaggc cgtgcgaagc gagcacctca gctacctcga
      301 tgccagcagc ctgcggaccc tcgccgcgac ggtcgagtcg atcgaggcgg acggcatcga
      361 cggcctgatc atcgagaccg gcaccgcccg cggtggttcg gcgatcacca tggcggcagc
      421 caagtcgacc acgcggcgga tgaaggtcta cgacgtgttc gggatgatcc cgccacccgg
      481 cgagaaggac ggcgacgacg tccatcggcg ctacgcgacg atcgccagcg gagcctcgaa
      541 gggtgtcggc ggcgagacgt actacggtta ccgcgaggac ctcctggccg aggtccgtga
      601 gtcgttcggg cggcacggcg tgccgatcgg ggaccacaac gtcgagttga tccaggggct
      661 gttcgaggac accctcacgc tcgacgagcc ggtcgctctg gctcacctcg acggcgactg
      721 gtacgcgtcg acgatgacct gcctgacccg gatcgctccg ttgctggtgg tcggcggtcg
      781 cttcgtgatc gacgactacg acacgtggtc cggttgccgc gccgccgtcg acgagtactt
      841 cgcccgccgc gacggctttc gcttcgaacg ccgcggcagg ttgcacatcg tccgtgtctg
      901 accgtcgatc ggccggggat cgctgaacgg cgcgtccgga cggcgcgggt atacttcacg
      961 ggctctcccg cccgacaccc gagcgcgcga tccgcccccg ccctgcgcgg agccgctgcg
     1021 tcccggcccg acccagccaa cgcgaaggaa ctgcccaccg tcatgctcga ccgctttttc
     1081 ggcatcttct cccgcgatat cgggatcgac ctcgggaccg ccaacaccct ggtccacgtc
     1141 cgcgaccgcg ggatcgtgat gagcgagccg tcggtcgtcg cgatcgatgc caagacgaag
     1201 cgggtgctcg ccgtcggggc ggaggccaag cggatggtcg gccggacgcc ggcgtcgatc
     1261 attgccgtcc gcccgctccg cgacggcgtg atcagcgact tcgacgtgac cgagcagatg
     1321 atcaagtact tcgtcgacaa ggtccacgac cggatcgggc tcatcgcccg gccacggatg
     1381 ctgctcggca tcccgtccgg cgtcaccgag gtcgagaagc gggccgtccg cgacgcggcc
     1441 atgaacgcgg gcgcgcgctg ggcccgcctg atcgaggagc cgatggcggc cgcgatcggc
     1501 gccgggctgc cggtcagcga gccgtcgggc tcgctcatcg tcgacatcgg cggcggcacg
     1561 accgaagtcg cggtcatcag cctcggcggg atcgtggtgt cgcgttcgat ccggatcggc
     1621 ggcgacgaga tggaccagga catcgtcgcc ttcgcccggc gcgagtacaa cctgttcctc
     1681 ggcgagcgga cggccgagga catcaagatc gcgatcgcgt cggcctaccc gggtgactgg
     1741 gacagccagc gcgtctcgct ccgcggccgc gacctgctga ccggcctgcc gcgcagcgtc
     1801 gaggtcggcg ccgaccagat ccgcgaggcg atcgagccat ccgtcgtcca gatcgtcgac
     1861 acgatcaagg acacgatcga ggagaccccg ccggagctcg tcgccgacat catggaccag
     1921 ggcatcgtcc ttgccggtgg cggtgctctc ctgtccggtc tcgaccggcg ggtcgccgac
     1981 gccacccaga tgccggtcca cgtcgcggac gatccgctga cctgcgtcgc ccgcgggacc
     2041 ggcgtcgtcc tccaggagct cgagaagatg gaccgcgtcc tcgtctccga cacgtacgcc
     2101 cgagcgccgc gctagaaggc ccgcccgggc gacctgctcg cggactgcgc gttagtctgg
     2161 cttcctcatg accaccatcc tcgccaaccg cgcgactcgc cgccgggcgg tgacgttcac
     2221 gatcctcctg gccgtcagcc tcatcctgat ggcgatctcc tccagccccg cggtgaccga
     2281 cttccagggc gccatggcat atgccctgcg tccgatccag ggcgcgatcc acggcgtcgc
     2341 ggattcggtc gcgaacgcgg tcgccgcggt cggcgagatc cagggcctgc actcggacaa
     2401 tgccgccctc cggcgcgaga accagcggct gaccgaggag aacctccggg cgaccgccat
     2461 cgagcaggag aacgagcagc tcaccgggct cctccagctc cgtaactcct acaagtacga
     2521 gacggctgcg gcggaggtta tcgcgcgcga gtccagcgag gcccgccgct ccgtcacgat
     2581 ctcgaagggg accgacgacg ggatcgcgac gggcgatgtc gtgatcgccg agggcggggc
     2641 cctggtcggc cgcgtcagcg atgtcggccc gaatttcgcg atcgtcaccc tgatcaccga
     2701 ccgcagctcg atcgtcatcg gcgagaccga gacgagcacc gccagcggcg aggtcgtcgg
     2761 ccagctcggc ggggcgctga tcatgcagaa catcgactcg accgagaagg tcgtgcccgg
     2821 cgagcaggtc ctcacgccgg gaaccgaggt cgcggggggg atccgttcgc cgtatccgaa
     2881 gggcctcgtg atcggcgagg tcagcgacat ccggaaggat gccaactcgg tcgtccagat
     2941 ggcctatctc ctgccgacga cggacctcga caaggtggcc tacgtcctcg tcatcctcga
     3001 ctatgccggt ggcctgccgg gcgtcgacga cacgccgatc gactgctccc agaccggcgt
     3061 gggcggcgcg ctgcccggcg gggagcaacc gtgcatccag ccgagcgcct cgccgcgggc
     3121 gacccctctg ctaccgtgac ggcgtgaaag gcatcgtcct cgccggcggg acggcaaccc
     3181 gtctgttccc cttgacgatc gtcacgaaca agcacctgct gccgatctac gaccggccga
     3241 tgatctacta cccgatcgaa accctggccg gcatgggcat ccgtgaggtg atggtgatcg
     3301 tcggcggcaa gagtgtcggc gacgtcgtcg agctgcttgc cgacggatcg catttcggcc
     3361 tcgacctgac gtatcgctac cagcatgggg cgctcgggat cgctcacgcg atcgggcttg
     3421 cccgggattt cgtcggcgac gattcgttct gctgcatcct tggcgacaac atcctgcgcg
     3481 ggccggccct ggccgccacg gcggccgaat tcgactcggg tccatggggc gcgggcacgc
     3541 tcctgtaccg ggtcgcggat cccgagcggt tcggcgtggc cgagctcgat accgacgggc
     3601 gtgtcatcgg cttcgaggag aagccggccc acccgaagag cgacctgatc ccgatcgggg
     3661 tctacttcct ccgtcccgac gcgttctcgg tcatcgacac ccttgccccg tcgggtcgcg
     3721 gcgaattcga gatcaccgac gtattgaatc actacatccc cgacgggcgc ctcttcagcc
     3781 gggtctacga cggcctctgg gcggacgccg gaaccgtccc ctcgctgatc cgggccgcgg
     3841 agctcgcccg cgacgacgac gacgcgggac ggctcgcccc gcccgtcgag cgcccgatcg
     3901 ggtaggcagg tcatgaccct cgagggcggg tcggcccgga tcgctcccgg cgcccggatc
     3961 ctcgtcaccg gcggggccgg gttcatcggc agtgcctacg tccgcgatgt cctcggccgc
     4021 ggcgacgcga cccggatcac ggtcctcgac aaactgacgt acgcgggcaa ccgcgccaac
     4081 ctcgcggcag tcgaggcgga ccccgggcag gcggcgcgat accgcttcgt ccagggcgac
     4141 atcgccgagc ccgggatcgt cggcccgctc gttgccgagg tcgatgcggt cgtgaacttc
     4201 gcggcggaat cgcacgtcga tcggagcatc ctcgacccgg aggcgttcct ccggacgggt
     4261 gtgatcggcg tccacgtcct gctcgaggcg gtccgggcgg cgtcgacggc tcgggcggcg
     4321 gccggcggga gccccgtccg ctacctccag gtgtcgaccg acgaggtcta cggttcggtc
     4381 gccgagggtc ggtcggtcga gaccgatcga ctcgatccgc gctccccgta ttcggcggcg
     4441 aaggccgccg gcgagctgct cgtggggagc tacgtcgtga cgcacggagt ggacgcggtt
     4501 gtcacgcgcg gatccaacac gtacgggcca ttccaccacc ccgagaagct gatcccgctg
     4561 ttcgtcacga atgcgatcga cgaccagccg ctgccgatgt atggcgacgg gttgcagcag
     4621 cgggactggc tgttcgtctc cgaccacgcc ggggcgatcg accacgttct ccgccgcggc
     4681 gcgagcggcg agacgtacaa cctcccgggt tcggccgaga tgacgaaccg cgccgtgatc
     4741 gccgcgctcc tcgagcggct gggcaagccc tggtcgctcg tccggaccgt cccggatcgg
     4801 ccgggccacg atcggcggta tgccctcgat ggaacgaagc tggcggcgct cggctggcgg
     4861 aaccggacaa agttcgcgga cggcctggcg gccacgatcg actggttccg ggccaacgag
     4921 gcgtggtggc gggcggcgaa gtccggcgac tgggacgcgt actacgagcg ccagtacggg
     4981 gcccgcctcg ccagcggcca ggccgtggcg caggtcagcg ccccgacgga agcctgagcg
     5041 tgcgcgtcgc ggtcaccggc gcgaccgggc ggctcggccg ggcagtcgtg acggctctcg
     5101 aggaggcgcc gttcaccggg ccgttcgggc cgatcgcctg gagccgggcc atcttcgatc
     5161 tcgatgctcc ggacagcgcc ggagcgctgt tcgatcgcga tcgaccggag gtcgtcgtcc
     5221 attgcgccgc ctggaccgat gtcgacggct gtgcccgcga tccgggtctc gcgctggccc
     5281 gcaatggaac ggcgaccgat gtgcttgccc gggccgcggc cgagcgcggg gtggacctca
     5341 tcgtcgtctc gacgaacgag gtcttcgacg gccgacggac cgaccggctc ggctacggac
     5401 cggacgacgc accgagtccg atcaacccgt acggcgcctc gaagctggcg ggggaggtgg
     5461 cggcgggcga cgcgttcgcg gccgcgtcgg gtggcgcggc cctcgggatc gcgcgaaccg
     5521 cgtggctgta cggcccgccc ggcaacgact tcccggccag gatcctcgcc gcagccgagc
     5581 gtgccaccgc gaccggtgag ccgctccgcc tcgtggccga cgagatcggg tcgccgagct
     5641 acaccctcga cgtggccgat gcgatcgccg agctgatcgg cgcggctgcg gtcgagggca
     5701 cccaccacct tgtcaacggc ggcacggcca gccgggcgga ttgggctcgg gagcttcttc
     5761 gccaggccgg cgtcgcggtc gtgaccgagg acgttccggc ggcgacatgg ccgcgcgcct
     5821 cgacgccgcc cgcgtgggcc gtgctcgagc cgaccccgtt gcccgggggc gagccgctcc
     5881 ggccgtggca ggcagccctc gccgactacc ttccgaccct gctccgggat cgcgcccggg
     5941 cgaaagccgg cgccggccga tgagcgatcc gcgcccgtcg ctcgagcgtt cgccatcggc
     6001 cctgccgggc gtccgctacg gcgcaatcgc ccgtcacgcc gacagtcgtg gggcgttccg
     6061 cgagctctgg cgcgcgtcgt ccttcccgat cctgacgccg gccgggaccg gggcgccggc
     6121 cgggaccgag ccgcggttcg tccaggcgaa ccagtcgagc tcggcagccg gcgtcctccg
     6181 cggcctgcat taccaccgcc accagctcga ctactgggtc gtcggatcgg gccgcgcgtt
     6241 cgtcgccctc gtcgacgtcc ggcgggtggt cggcggcgtc gggccggccg tcgtcgagac
     6301 gcgtgagctg gacgccggcg aatgggtcgt catcccggcc ggcgtcgccc acggcttcct
     6361 cgccctcgaa ccgctcgagc tcgtctattt cgtcacgaac gagttcgacg gcagtgacga
     6421 gctaggcttc gcctgggacg acccggccgt cgccgtcccg tggccggccg tcgacgcgac
     6481 ccccgacgac cgcccgatcc tgtccgaacg ggaccaatcg aacccaccgt tggcggagct
     6541 ggtggccagc ctccgcggct gagcgactca ctcggcgacg cgtgcggacg cgaatgccgg
     6601 tctcgccatt cactacgccg cgtcgtggac gtcaccgccg atggcctgat tcggccccgc
     6661 ccgggcgcga tttccctgga tggcgcccga tcctccgaca ttcaccacgc ccgacagtga
     6721 gtgacaaccg cgggtcgccg ccccgcggac ttgcaccacg cccggttgtg aat
//
LOCUS       DATAIW010000479         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_230788, whole genome shotgun sequence.
ACCESSION   DATAIW010000479 DATAIW010000000
VERSION     DATAIW010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230788"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VHM48_14240"
     CDS             complement(<1..217)
                     /locus_tag="VHM48_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2756624.1"
                     /translation="MRFTRALALGASIVVLVSACSTGGGGKPTVKVGSDGFYEAKVMA
                     EIYAQALEAGGYTVDRTGIGIGARKVSA"
     gene            complement(329..1066)
                     /locus_tag="VHM48_14245"
     CDS             complement(329..1066)
                     /locus_tag="VHM48_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2756625.1"
                     /translation="MNLVTATAAWLTDPAQWAGQAGIPNRLLEHVLIASISLAIALLL
                     ALPAGLAIGHTGRGAAVAASVANLGRSLPSLAVIAIAIPVTVLIDPVVGGAVLPTLVA
                     MVVLAVPPILVNTYTGIAEVDRDLVESGRGMGMREREILAGIELPVALPVVLGGIRSA
                     AVQIVATATLGAIFGGGGLGRFLVDGIAQKDDGQVFGGVLLVAALAIATEVAVSLLQR
                     VARSPGLGGDGNGPAVELAPQPPVLGM"
     gene            complement(1063..1725)
                     /locus_tag="VHM48_14250"
     CDS             complement(1063..1725)
                     /locus_tag="VHM48_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756626.1"
                     /translation="MIDWGWLAGHLDAIAYRTAQHVYLAALGVAIGFAISFVLALVAA
                     RHRALYPPISALAGILYTIPSLALFAALISITGFSVVTAEVPLVMYTLVILVRNIVAG
                     LDGVPAEVLEAADGMGYTRRQRLWRVELPLAVPLIVAGLRLATVSTIGLVTITGTISD
                     AFGGLGYFILDGYHRGTFLTEMYAGALPSILLALGADFLFVRVQRRVTPWAQARAIEA
                     TS"
     gene            complement(1745..>2074)
                     /locus_tag="VHM48_14255"
     CDS             complement(1745..>2074)
                     /locus_tag="VHM48_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2756627.1"
                     /translation="RARTFADPFPYLLLLDPSDRPIGWVNQRHIPGTGTLAPSLATPM
                     SPLFDRRTTLKDALSMMLDADVQTGIVVDRAGRLRGVLTVEAIAAVLRPSADPEVIAA
                     GASGADA"
BASE COUNT          337 a          735 c          748 g          254 t
ORIGIN      
        1 gggcggagac cttgcgggcc ccgatcccga ttcccgtccg atcgaccgtg tagccaccgg
       61 cctcgagagc ctgcgcgtag atctcggcca tgaccttggc ttcgtagaag ccgtccgatc
      121 cgaccttgac ggtcggcttg ccgcccccac ccgtcgagca ggcgctcacc agcaccacga
      181 tcgacgcccc gagggcgagc gcgcgggtga accgcatggg tcgcctcctt actgtgcccg
      241 tgtccgcctg ggaggctgca tcgggtcacg cggactatat cggcccgcgc ccgatcgctc
      301 cgaatccgtc cccgcggggc ccggaacgtt acatcccgag cacgggcggc tgcggcgcga
      361 gctccacagc cggcccgttc ccatcgccgc ccagccccgg cgaccgggca acgcgctgca
      421 ggaggctgac ggcgacctcg gtcgcgatcg cgagggccgc gaccaggagc acgccaccga
      481 acacctggcc gtcgtccttc tgggcgatcc cgtccacgag gaagcgcccg agcccgccgc
      541 cgccgaagat cgcccccagg gttgccgtcg cgacgatctg gacggctgcg gagcgaatcc
      601 ccccgaggac aacgggaagc gcaaccggca gctcgatccc ggccaggatc tcgcgctcgc
      661 gcatgcccat cccgcggccg gactcgacga gatcccggtc gacctccgcg atgcccgtat
      721 aggtattgac gaggatcggc ggcacggcga gcacaaccat cgccacgagc gtcggcagga
      781 cggccccgcc gacgaccggg tcgatgagca ccgtgacggg gatggcgatc gcgatgaccg
      841 cgagcgaggg cagcgagcgc ccaaggtttg ccacgctggc cgcgacggcg gcgccccggc
      901 ccgtgtggcc gatcgcgagt cccgccggga gcgcgaggag cagcgcgatc gccagcgaga
      961 tgctggcgat cagcacgtgc tccaggagcc ggttcgggat tccggcctgg ccggcccact
     1021 gggcgggatc cgtgagccag gcggccgtcg ccgtcacgag gttcacgacg tcgcctcgat
     1081 cgcccgcgcc tgggcccagg gtgtgacacg ccgctgcacc cggacgaaca ggaagtcggc
     1141 gcccagcgcc aggaggatcg agggaagcgc cccggcgtac atctcggtca ggaaggtgcc
     1201 gcggtggtag ccgtcgagga tgaagtagcc cagcccgcca aacgcgtcgc tgatcgtccc
     1261 ggtgatcgtg acgaggccga tcgtggagac cgtggccagc cggagcccgg cgacgatgag
     1321 cgggaccgcg agcggcagct ccacgcgcca gagccgctgg cggcgcgtgt aacccatccc
     1381 gtcggccgcc tccaggacct cggccgggac gccgtcgagg ccggcgacga tgttccgcac
     1441 gaggatcacc agggtgtaca tgaccagcgg cacctcggcc gtgacgaccg agaacccggt
     1501 gatggagatg agggcggcga agagcgcaag gctcgggatc gtgtagagaa tcccggcaag
     1561 cgcgctgatc ggcgggtaga gggctcgatg gcgggccgcc acgagcgcga ggacgaacga
     1621 gatcgcgaac ccgatcgcca ccccgagcgc cgccaggtaa acgtgctgcg cggtccggta
     1681 ggcaattgcg tccagatggc cagccagcca gccccagtcg atcatcgcgc ctgcggcctg
     1741 cggctcacgc gtcggcgccg gacgcgccgg cagcgatgac ctcgggatcg gcgctcggac
     1801 ggagcacggc cgcgatcgcc tcgacggtga gcacgccgcg gagcctgccc gcacggtcca
     1861 cgacgatccc cgtctggacg tccgcgtcga gcatcatcga gagcgcgtcc ttgagggtgg
     1921 tgcgtcggtc gaacagcggt gacatcggtg tcgcgagcga cggcgcgagg gtgcccgtcc
     1981 cggggatgtg gcgttggttt acccagccga tcggtcggtc cgacgggtcc agcaggagaa
     2041 ggtacgggaa cgggtccgcg aacgtccggg cccg
//
LOCUS       DATAIW010000480         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_291020, whole genome shotgun sequence.
ACCESSION   DATAIW010000480 DATAIW010000000
VERSION     DATAIW010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291020"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VHM48_14260"
     CDS             <1..1460
                     /locus_tag="VHM48_14260"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2756628.1"
                     /translation="LEVHNVRRGIFDTEVVRTWERRSTALDVVGDALFLVFAQDFAPL
                     EERLDAIASRLGAVPAYLAQSRDRAVVPQVRAWQQLEIESAADMPSFFDEIVGAGAGL
                     PEPEQRRLAAAATAARAAIGEYGAWLKESLAVGTNDWALGREPYDELVDLRAFDGLDA
                     DAILEIGEDQLEMNKAARIRAAAEIDDRSTETEVIDRLKSDHPATFAEALDAYRDVML
                     RARQHLIDHDIVTVPPDERIEVIATPDYLRNVIPFAAYFSPPKFDPRPKGIYIVTPSV
                     GNDPNAMREHNFSSISNTSIHEAYPGHHLQLSVANSHPSMTRLLTDAPEFVEGWGMYS
                     EQMMREQGFDAAANFRLNLHTDAIWRACRIILDVRMHRGEVAVDEAIRFLVEQTSFEE
                     ANARAEVLRYTYTPTYQLSYLLGKVLLLGLRADEQARLGSAFSLRDFHDTLLRNGSLP
                     ISFHRRIMAERVAAGSAGGAGTAGGASGANRAARP"
     gene            1462..2163
                     /locus_tag="VHM48_14265"
     CDS             1462..2163
                     /locus_tag="VHM48_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2756629.1"
                     /translation="MQVIPSIDLDRGRSRVVFWPGASTGIGAPTDRPERIAESFVERG
                     ARLIHLVDFDGARTGRPGNLEIVGTIASRIAIPIQVAGGVDSADGIRLAFAAGATRVV
                     TSMAIADDPTALAEGLEVAGAWLAVGLDPRPERLAAFPWQRAGSPTLAGVVTELADRG
                     VTRFVLAHGGHAPDLVVLGPLAARGDIDVLVAGGVTDLDGIRRLRDAGIAGIILGEAL
                     LSGAIDFTAALEAAA"
BASE COUNT          327 a          858 c          777 g          331 t
ORIGIN      
        1 atctcgaggt ccacaacgtc cgccgcggca tcttcgacac cgaggtcgtc cggacctggg
       61 agcgccgctc gacggcgctc gacgttgtcg gcgacgcgct gttcctcgtc ttcgcccagg
      121 acttcgcccc gctggaggag cgcctcgacg ccatcgccag ccggctcggg gcggttcccg
      181 catacctcgc ccagtcgcgc gatcgagcgg tcgttcctca ggtccgggcc tggcagcagc
      241 tcgagatcga atcggccgcc gacatgccgt cgttcttcga cgagatcgtc ggcgcgggcg
      301 ccggcctgcc cgaaccggag cagcgccggc tcgccgccgc cgccactgcc gcgcgggccg
      361 cgatcggcga gtacggcgcc tggctgaagg agtcgctcgc ggtcggcacg aacgactggg
      421 cgctcgggcg cgagccttac gacgagctcg tggacctgcg tgccttcgac ggcctcgacg
      481 ccgacgcgat cctcgagatc ggcgaggacc agctcgagat gaacaaggcc gcccggatcc
      541 gggccgccgc cgagatcgac gatcgctcga ccgagaccga ggtgatcgac cgcctgaagt
      601 cggaccatcc ggcgacgttc gccgaggccc tcgacgcgta tcgcgacgtc atgctccggg
      661 cccgccagca cctgatcgac cacgacatcg tgaccgtccc gccggacgag cggatcgagg
      721 tcatcgcgac gcccgattac ctccgcaacg tcatcccgtt cgcggcctat ttctcgccgc
      781 cgaaattcga cccgcggccc aagggcatct acatcgtcac gccgtcggtc ggcaacgatc
      841 cgaacgcgat gcgggagcac aacttcagtt cgatcagcaa caccagcatc cacgaggcgt
      901 atcccggcca ccacctccag ctgagcgtgg ccaacagcca tccgtcgatg acccggctgc
      961 tgaccgacgc gcccgagttc gtcgaaggct gggggatgta ctccgagcag atgatgcgcg
     1021 agcagggctt cgacgcggcg gccaacttcc gcctgaacct ccacaccgac gcgatctggc
     1081 gggcgtgccg gatcatcctc gatgtccgga tgcaccgcgg cgaggtcgcc gtcgacgagg
     1141 cgatccggtt cctcgtcgag cagacgagct tcgaggaggc gaacgcccgg gcggaggttc
     1201 tccgctacac ctacaccccg acctatcagc tgtcgtacct gctgggcaag gtgctcctgc
     1261 tcggcctgcg cgccgacgag caggcccgcc tcggcagcgc cttctcgctc cgtgacttcc
     1321 acgacaccct gctccggaac gggtcgctgc cgatcagctt ccatcgtcgg atcatggctg
     1381 agcgcgtcgc ggcgggatcg gccggcgggg cgggtaccgc aggtggcgcc agcggcgcga
     1441 accgcgccgc gcgcccctga cgtgcaggtc attccctcga tcgacctcga ccgcggccgc
     1501 agccgggtgg tcttctggcc gggcgcgtcg accggcatcg gcgcgccgac cgaccgcccg
     1561 gagcggatcg ccgagagctt cgtggaacgt ggcgcgcggc tcatccacct cgtcgacttc
     1621 gacggggccc gaacgggtcg gccgggcaat ctcgagatcg tcgggacgat cgcatcgcgg
     1681 atcgcgatcc cgatccaggt tgccggcggc gtcgacagcg ccgacgggat ccggctcgcg
     1741 ttcgcggccg gcgcgacgcg ggtcgtgacg tcgatggcga tcgcggacga cccgacggcc
     1801 ctggccgaag ggctcgaggt cgccggcgcc tggctggcgg tcgggctcga cccgcgcccg
     1861 gagcggctcg ccgcgtttcc ctggcagcgc gccggatcgc cgacccttgc cggggtcgtc
     1921 acggagctgg ccgatcgcgg cgtcacgagg ttcgtcctcg cccacggcgg tcacgcgccg
     1981 gacctcgtcg tgctcggccc gctggccgcc cgtggcgaca tcgatgtcct cgtcgccggc
     2041 ggcgtgaccg acctcgacgg catccgtcgc ctgcgcgacg cggggatcgc gggcatcatc
     2101 cttggcgagg cgctcctctc gggggccatc gacttcaccg ccgccctgga ggcagccgct
     2161 tgagctccga tcagaatcgt ccgcgtccac gctcgtcgaa gaagcgacga gccgtccagc
     2221 ccggagctgc cggtcgcaac ccgtcccagc gcggaccggc cacccggcgc gtcagcggcg
     2281 aacccgtcgg tcc
//
LOCUS       DATAIW010000481         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_231076, whole genome shotgun sequence.
ACCESSION   DATAIW010000481 DATAIW010000000
VERSION     DATAIW010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231076"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VHM48_14270"
     CDS             complement(<1..356)
                     /locus_tag="VHM48_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756630.1"
                     /translation="MKLYKVGALAAVSILAFAACSSSSSGSAAPSSAASGAGTASKQD
                     AVCKDKVGKSTTEIHVYSSLPLEGTSLPQSTAIVNAIKETLDGQKIGNFAIKYISLDD
                     ASAAKNGDWDGAVEQA"
     gene            838..1188
                     /locus_tag="VHM48_14275"
     CDS             838..1188
                     /locus_tag="VHM48_14275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756631.1"
                     /translation="MRQLVYLPQASIVVAETQRHVVGGAILALRPSVRAGGYVGTIDL
                     LVVDPEYDADRITEALLEELLRSASNKGCTVVEADRPDDDTLARWQRMGFAEAGPRIQ
                     RTVTAAGAAARRTI"
     gene            1250..>1949
                     /locus_tag="VHM48_14280"
     CDS             1250..>1949
                     /locus_tag="VHM48_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2756632.1"
                     /translation="MGKNVAVFVDVANIFYAAKAAGVDIDYVTLLKAASAGRDLVRAY
                     AYTGLDPDNENQRNFHDFLRRSNYKVVSKDIRKYGDGKVKANLDIELVVDMMKTARNL
                     DIAIVVSGDGDFAPAIRAVQEQGVRVEVISFRGNTSSDLIEVADQFTDIVQLARVEKG
                     TRSGRRVADDDADLSMTEVPDKLTEGTGAGRGRGRGRGRGREAEPVAATSRGRSRIRT
                     STAEPAPAGARTEAA"
BASE COUNT          327 a          650 c          658 g          314 t
ORIGIN      
        1 ttggcctgct cgaccgcgcc gtcccagtcg ccgttcttgg cggccgaggc gtcgtcgagg
       61 ctgatgtact tgatggcgaa gttgccgatc ttctgcccgt cgagggtctc cttgatcgcg
      121 ttcacgatcg cggtgctctg cgggaggctc gtgccctcga gcgggaggct cgagtagaca
      181 tggatctcgg tcgtgctctt gccgaccttg tccttgcaga cggcgtcctg cttgcttgcc
      241 gtgccggcgc cactagctgc cgagctcggc gcggccgagc cgctgctgct tgagctgcac
      301 gccgcgaacg ccagaatact gacggccgca agtgcgccta ccttgtacag tttcatgcga
      361 tctcctccaa atcaccggtc gtaaccacct ggcacgatcc ggcgcgagga ccccctgtcg
      421 aactctcggt acccctccgc cgggccccgc tgccctccct gacggcggtt cacggacgat
      481 ggtggatcgg tgagcgacga cgatcgggtc ccgcatccgg gcggcgcaca ggtggccgcc
      541 gaagcgatgc cgtatattac gcaccggcga cctgaatgca ataccggtcg gccgcaccct
      601 caagcgcgtc gtgggcgatc catcgctcgc cgcagatgag cggatcgcgg gcctcggtag
      661 cgcctcggtc gaacccgatc gagggccatg gtcgccgaac cctgcgaggt aggacccgtc
      721 ggcgtggaat acctggtccg agcggcccgt gtcacggaca tcgatcgagt cgtcgcgttg
      781 tgcgacgatc ccgtggagat gagccgccgt gagagtccgc tcggggcggc cgatctcatg
      841 cgccagttgg tctacctgcc gcaggccagc atcgtcgtcg ccgaaacgca gcgtcacgtc
      901 gtcgggggcg cgatcctcgc gctccggcca tccgttcggg ccggcgggta cgtcggcacg
      961 atcgacctcc tggtcgtcga cccggagtac gacgccgatc gcatcaccga ggcactcctg
     1021 gaggaactgc tgagatccgc cagcaacaag ggttgtaccg tcgtcgaagc cgatcggcct
     1081 gacgacgaca cgctcgcgcg ctggcaacgg atgggtttcg ctgaagctgg tccgcgcatc
     1141 caacgaaccg tgaccgcggc gggagccgcc gcgcgtcgca cgatatgaac gtcctcgccg
     1201 agctgcgtga tcgcgccggc cgggcgaaac cctaaaccaa ggagtcaatg tgggcaagaa
     1261 cgtcgccgtc ttcgtggacg tggcgaacat cttctatgcg gccaaggccg cgggcgtgga
     1321 catcgattac gtcacgcttc tcaaagccgc ctcggccgga cgtgatctgg tgcgcgcgta
     1381 tgcgtacacg ggcctcgatc cggacaacga gaaccagcgc aacttccacg atttcctgcg
     1441 ccggagcaac tacaaggtcg tcagcaagga catccgcaag tacggcgacg gcaaggtcaa
     1501 ggcgaacctc gatatcgaac tggtcgtcga catgatgaag accgcccgga acctcgatat
     1561 cgccatcgtc gtgtccggtg acggcgactt cgcgccggcg atccgcgccg tccaggagca
     1621 gggcgtccgg gtcgaggtga tcagcttccg cgggaacacg agttctgacc tcatcgaagt
     1681 ggccgaccag ttcacggaca tcgtccagct tgcccgggtc gagaagggca cgcggtcggg
     1741 ccgccgggtg gcggacgacg atgcggatct gtcgatgacc gaggttccgg acaagctcac
     1801 ggaagggacc ggcgccggtc gcggtcgcgg gcgagggcgg ggacgcggac gcgaagccga
     1861 gccggtcgcc gcgacgagcc ggggacgcag ccgtatccgc acgagcacgg cagagccggc
     1921 tcccgccggc gcacgcaccg aggcggccg
//
LOCUS       DATAIW010000482         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_201110, whole genome shotgun sequence.
ACCESSION   DATAIW010000482 DATAIW010000000
VERSION     DATAIW010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201110"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VHM48_14285"
     CDS             complement(<1..1787)
                     /locus_tag="VHM48_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2756633.1"
                     /translation="MTAYAELHCHTNFSFLDGASAPDELAERAAELGLGGLAVTDHQG
                     LYGVVRASAAYEEAGLLPVLGIEVELRDAIVADPERAVVPARQAVRRGGRRRSAEAGA
                     VDESAAREGEPDRPRPERARLPGHREAVKEDRRGIGETQRGPHLVLLARNAVGYRSLC
                     RLVSRANLAGTKAMPRFDHALLAAHAEGLVALSGCRDGEIARRLRVGDRDGARAAARA
                     FAAVYGGPDGDGFHLELAHHHLPDDDWLVTETAALAEELDLPVVVTNDVHYALPEGRE
                     FQDVLTAIRHGRTLDGLADLRRPDGESYLKSAAEMAALPPGDGSLGRDVARAWAAGME
                     RSVELSATCRVDLGFEQYRFPGFPVPKGETPFSYLSGLCWDGAKKRYHPLTSAVVNRL
                     AHELDVIERAGLAEFFLICWDLMRFAKSQGIPAQGRGSAAGSIIAYTLGISRVEPIQH
                     NLLFERFINEGRTTYPDVDIDFSSARREEVIQYVYGRYGPEHTGMVANLVTYRARSAV
                     REVGVALGFPRPLVDRVAKALETYDSVMVRRDLEADGGFAEFFKRPGEGEPVEAAAAA
                     VAGDRGLVDGMGQLNHARGGRVRTATGSGT"
     gene            1945..2070
                     /locus_tag="VHM48_14290"
     CDS             1945..2070
                     /locus_tag="VHM48_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756634.1"
                     /translation="MKRDTASLVAATLRTGLLVGIALILILILFPAAMAQAAGLR"
     gene            2280..2816
                     /locus_tag="VHM48_14295"
     CDS             2280..2816
                     /locus_tag="VHM48_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756635.1"
                     /translation="MTALRRWAADPLVPILAITLALLAVGCGPSASTTLKPSASPRAS
                     ASPHLTAVPGGPASPVAGASVGPPTTTETEFGTIWDALPPSFPRLPGQEPADTGAGPT
                     SGSFALNMTAAAASQAIAAALKALGWTVDVGSPLEDGSVVLDATHAPAGCKTEVRFTP
                     LSGTVIMSVLYGASCPFS"
     gene            complement(3362..3610)
                     /locus_tag="VHM48_14300"
     CDS             complement(3362..3610)
                     /locus_tag="VHM48_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756636.1"
                     /translation="MTRLLREHPPIDVELDGDGRLVAIRWNGQREPVEVCNAWRVAES
                     WWREPIERDYCKVVGTRWLALVYLDRVDGTWHLERLYD"
     gene            complement(3607..4839)
                     /locus_tag="VHM48_14305"
     CDS             complement(3607..4839)
                     /locus_tag="VHM48_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX2756637.1"
                     /translation="MRLLYLYWPHLPIRLARSRHSDSFPTDRPVVLGGQPWTDGLVID
                     ADPAARALGVRRGIPLGSAHRLAPEAAFLDPETDADSAAVEAAFETLARFSPGLAGTT
                     DPTDPAFGLIELQADGLDRLWGPDVVLVGRLVEALATILPGAPRPGIAGTRFAATVAA
                     GHAEPAVARIVEPGGDAGFLAPLPSSLLSPDPDVRARLARFGLRRIGAVAELPRSALV
                     ARFGEEGGRIHARARGEEIERFRPRRAPERLILALPIDPPVAETESLRFILHRLAGAL
                     AAQVDARGMAAARATLRLALEMAFARAGTPGELVVEQRFPEPTADADAIERLLLARLE
                     QAPPPAAVERLELELAGVEPAVGQQLPLFVPQAARTARLGWQLARLALTFGEDRVRKV
                     AITDPDAPLAEKRWNWRS"
BASE COUNT          666 a         1818 c         1730 g          655 t
ORIGIN      
        1 gtgccgctcc ccgtcgcggt ccggacccgc ccgccgcggg catggttcag ctggcccatt
       61 ccgtcgacca gcccacggtc cccggccacc gctgccgcgg ccgcttcgac cggctccccc
      121 tctccgggcc gcttgaagaa ctcggcgaac ccgccgtccg cctcgaggtc gcgccggacc
      181 atcaccgagt cgtatgtctc gagggccttc gccacccggt ccacgagcgg ccgtgggaac
      241 cccagcgcga ccccgacctc ccgcaccgcc gaccgcgccc ggtacgtcac gaggttggcg
      301 accatcccgg tgtgctcggg gccgtagcgg ccgtagacgt actggatgac ctcctcgcgc
      361 cgcgcggacg agaagtcgat gtcgacgtcc ggataggtcg tccggccctc gttgatgaac
      421 cgttcgaaca ggaggttgtg ctggatcggc tcgacccggc tgatcccgag ggtgtaggcg
      481 atgatcgaac cggcggcgct cccccgcccc tgggccggga tcccctggct cttcgcgaag
      541 cgcatcaggt cccagcagat gaggaagaac tcggcgaggc ctgcccgctc gatcacgtcg
      601 agctcgtggg ccaggcggtt gacgacggcc gaggtcagcg ggtgatagcg cttcttcgcg
      661 ccgtcccagc acagccccga gaggtacgag aagggggtct cgcctttcgg caccgggaag
      721 cccgggaagc ggtactgctc gaacccgaga tcgacccggc aggtggccga cagctcgacc
      781 gaccgctcca tccccgccgc ccacgcccga gccacgtcac gcccgagcga gccgtcgccg
      841 ggcggcagcg ccgccatctc ggccgccgac ttcaggtacg actcgccatc gggccggcgc
      901 aggtcggcga gcccgtcgag cgtccggccg tggcggatcg cggtcaggac gtcctggaac
      961 tcgcggccct cgggcagcgc gtaatggacg tcgttcgtga cgacgaccgg caggtccagc
     1021 tcctcggcca gagccgctgt ctcggtcacg agccagtcgt cgtcgggcag gtgatggtgc
     1081 gccagctcga ggtggaagcc gtcgccgtcc ggcccgccgt agaccgccgc gaacgcccgc
     1141 gccgccgccc gcgccccgtc gcgatcaccg acccgcagcc gccgcgcgat ctcgccgtcg
     1201 cgacagcccg acagggcgac gagcccctcg gcgtgggcgg cgagcagggc gtggtcgaac
     1261 cgcggcatcg ccttcgtccc ggcgagattg gcccgcgaga cgagccggca caggctccga
     1321 tagccgaccg cgttgcgggc gagcaggacg agatgcggtc cgcgctgcgt ctcgccgatc
     1381 ccgcggcgat cctccttgac cgcctcgcgg tggccgggca gacgggcccg ctccggtcgc
     1441 ggccgatccg gctctccctc gcgagccgcc gactcgtcca ccgcgccggc ctcggcggat
     1501 cgacgacggc ctccccggcg gaccgcctgc cgcgccggca ccacggcccg ctccgggtcg
     1561 gcgacgatcg catcccgcaa ctcgacctcg atcccgagga ccggcaacag ccccgcctcc
     1621 tcgtacgcgg cgctcgcccg gacgaccccg tagaggccct ggtggtcggt cacggccaac
     1681 ccaccaaggc cgagctcggc ggcccgttcg gccagctcgt ccggcgccga ggcgccgtcg
     1741 aggaacgaga agttggtgtg gcaatggagc tcggcgtagg cggtcatctg gaccgtcgat
     1801 gatcgaacgg atgttcgatt tggtcaagtg gcggatccgt ccgacggacc tcccggccgg
     1861 acggaagtcc cccctcggaa tgggccagaa atcgtgctcc ggacaacgca acagaatcgg
     1921 caagtccagc gtctacacgg acacatgaag cgcgacaccg cgagcctcgt cgccgccact
     1981 ctccggaccg gtctcctggt cgggatcgcc ctgatcctca tcctgatcct cttcccggcg
     2041 gcgatggccc aggcggccgg tctgcgctga taccacatac cgtcctggcc tgggggctcg
     2101 gggcgaactt gggcggctac cggtcgcgag gaccggtgcc gacccgctgc aggatctggt
     2161 gagcgggtcc ggcggccagg gaccgtcgtc gtgctggggc atggcgacgg tcccttcgtg
     2221 ttctggccgg gcctccggcg tcgccgacga catcccgatc gcccggctac gatgagccgg
     2281 tgaccgccct ccgccgctgg gccgccgatc ccctcgtccc gatccttgcg atcacgctcg
     2341 ccctgctcgc cgttggctgc ggcccgagcg cgtcgacgac gctgaagccg tccgcgagcc
     2401 cgcgcgcgtc cgcatcgccg cacctcaccg ccgttcccgg cggcccggcc agcccggtcg
     2461 ctggcgcctc ggtcggcccg ccgacgacga ccgagaccga gttcggaacg atctgggacg
     2521 ccctgccgcc atcattcccc aggctgcccg gccaggagcc ggccgacacg ggcgctggac
     2581 cgacgagcgg atcgttcgcg ctgaacatga ctgcggcagc cgccagccag gcgatcgcgg
     2641 cggcgctgaa ggcgctgggc tggacggtcg acgtcggctc gcccctcgag gatggctcgg
     2701 tcgttctgga cgccacgcac gccccggcag ggtgcaagac cgaggtgcgc ttcacgccgc
     2761 tgtctggcac agtcatcatg tcggtgctgt acggcgcgtc ctgcccgttc agctgatcgc
     2821 acgggagacc agatcggtgc atgtgacggc tcgcgatatc gcgggcgctc tcgtcctgac
     2881 ggccgttctc gtcggtggcg tcgtcttcct cgtgctcggc tcgatgcact gaggccggcg
     2941 ggcaacccca cccgttccga cccgtcgcca acccacggtg acgatcgcaa cgtcgttgat
     3001 ccgcggaaac ctgaatcgcc aagtccgcgt aacgctccct gaacggccgc cgtctacgat
     3061 tccgcggccg gcaggggacc ggctccgatc aggggtgatg gcgtgcagca ccgatccacg
     3121 cgctccagcg atgtccaggc gctcgccttc gtggtggcgc tcttcggcct cctcctcgcg
     3181 ctgaccgtga tgctcggagc gccgatcgcc tgagggtttc gatcgtcgcg cagcggggcg
     3241 atttcgcggt cgttgcgacc aagcgaaacg agttcggccg caaacaagtt ccacttgagc
     3301 gaaacggtcg cgaaattggg tttgtgggct tgcgagctgg gccgtgcttg cgcggcggcg
     3361 tctagtcgta gagccgctcg aggtgccacg tcccgtcgac ccggtcgagg tagacgaggg
     3421 ccagccagcg cgtcccgacg accttgcagt agtcgcgctc gatcggctcg cgccaccacg
     3481 attcggcgac ccgccaggcg ttgcagacct cgaccggctc gcgctggccg ttccagcgga
     3541 tcgcgaccag ccgtccgtcg ccgtccaatt cgacgtcgat cgggggatgc tcacggagga
     3601 ggcgggtcat gagcgccagt tccaacgctt ctccgcgagc ggcgcgtcgg gatcggtgat
     3661 cgcgaccttg cgaacgcggt cctcaccgaa cgtcagggcc agccgggcca gctgccagcc
     3721 gagccgcgcg gtccgggcgg cctgcggcac gaacagcggc agctgctgcc cgaccgccgg
     3781 ctcgaccccg gccagctcca gctccaggcg ctcgaccgcg gccggcggcg gcgcctgctc
     3841 gagccgggcg aggaggaggc gctcgatcgc gtccgcgtcg gcggtcggct cggggaagcg
     3901 ctgctcgacg accagctccc cgggcgtgcc cgcgcgggcg aacgccatct cgagcgccag
     3961 gcgcagcgtc gcccgcgccg ccgccatccc ccgggcgtcg acctgggcgg cgagcgcgcc
     4021 ggccagccgg tggaggatga accggagcga ctccgtctcg gcgacgggcg ggtcgatggg
     4081 gagcgccagg atcagccgct ccggggcgcg ccgcggccgg aaccgctcga tctcctcgcc
     4141 ccgggcccgg gcgtggatcc gcccgccctc ttcgccgaac cgggcgacaa gcgcgctccg
     4201 gggcaactcc gccacggcgc cgatccggcg cagcccgaac cgggcgaggc gggcacggac
     4261 atccggatcc ggcgagagga gcgacgacgg caggggcgcg aggaatccgg cgtccccgcc
     4321 gggctcgacg atccgggcga cggccggctc ggcgtgaccg gcggcgaccg tggccgcgaa
     4381 ccgggtcccg gcgatccccg gtcgcggggc cccgggcagg atcgtcgcca gcgcctcgac
     4441 caggcgcccg acgaggacga cgtccgggcc ccacagccgg tcgagcccgt cggcctggag
     4501 ctcgatcagc ccgaacgcgg gatcggtcgg gtcggtcgtg ccggcgagac ccgggctgaa
     4561 ccgggcgagc gtctcgaagg cggcctcgac ggccgcgctg tcggcgtcgg tctccggatc
     4621 gaggaacgcc gcctcagggg cgaggcgatg ggccgacccg agcgggatcc cgcgccggac
     4681 gccgagcgcc ctggccgccg gatccgcgtc gatcaccagc ccatccgtcc acggctggcc
     4741 gccgaggacc accggccggt cggtcgggaa cgagtcggag tggcgcgacc gggccagccg
     4801 gatcgggagg tggggccagt agaggtagag gagtcgcatc ggtcgggatc cgtgggacgg
     4861 ggctggcgg
//
LOCUS       DATAIW010000483         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_201236, whole genome shotgun sequence.
ACCESSION   DATAIW010000483 DATAIW010000000
VERSION     DATAIW010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201236"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1317
                     /locus_tag="VHM48_14310"
     CDS             322..1317
                     /locus_tag="VHM48_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2756638.1"
                     /translation="MTDLISAQGLVKIYKGRKSEVRALDGVDLAVPEGTVLGLLGPNG
                     AGKTTAVRILATLLKPDAGHATVAGHDVVREPDAVRRVMGLSGQYAAVDENLTGNENL
                     WLFGRLYRLSSPEAHRRANELLGDFDLLDAGDRVVKTYSGGMRRRLDLASALIGRPRL
                     LILDEPTTGLDPRSRLGMWDVLRGLVRDGVTLLLTTQYLEEADELADTIAVVDHGKII
                     ARGTADELKSQVGGERIEVVVRDRESIAAARELLARDAGGDTTLDEHTRKLTVPAHGG
                     AQTLVQVVRDLDEAGIHIDDIALRRPTLDDVFLALTGHASAEDSTTDAPTSGRAA"
     gene            1314..2135
                     /locus_tag="VHM48_14315"
     CDS             1314..2135
                     /locus_tag="VHM48_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756639.1"
                     /translation="MTTTTADRRPGAVRAAVTDGLLVGWRNLKRIPRIPELAIFAVLQ
                     SIMFVLLFAFVFGGAIPIPGGGDYKAYLMPGIFAQTIVFAAGTTAIGMADDVHKGIID
                     RFRSLPMSRSAVLTGRTFSDVIYNAMILVVLMVTGFVIGWRVESIPALVAGFLLLLFF
                     AYGMAWLGVWLGLNVPTVEVGQQVIFTTLFPITFISNVFVPLQGLPSWLQPVAEWNPT
                     STLTAALRQLWGNPNPATSNSLAAQQPLLVTIGWVVVIIAIFGPLGVRRYRSMSR"
     gene            2137..>2350
                     /locus_tag="VHM48_14320"
     CDS             2137..>2350
                     /locus_tag="VHM48_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756640.1"
                     /translation="MDDEELRPELRRSRRAPAPGSRAADADAAGPVPDGPIVAANQAA
                     PGPANARWQALLAEGGPILADGAMGTM"
BASE COUNT          325 a          870 c          799 g          356 t
ORIGIN      
        1 gccgatcggg gccggcgtcg cgcagccgcc gagcatgttg gtcgcgatcg cgatcgcgac
       61 cgcggccaac caccctcgcg cggtcccgcg atgacccctg ccgcgcatcg tcgtcgtcat
      121 cggaacctcc ctccggacgc cgggcgcggc caatgccgtc tgacgtctcg aagcgatgac
      181 gcccgagaag gccgcgcgtg tcggcccggg tcgacggttg tcggctcgtc gacaaggagg
      241 cccgggtccg cgatcccgat cctcggtaca ctcccacatc cgcgccggat gcccactcgc
      301 cggctcgctc gaggatcccg gatgaccgat ctgatctccg cccagggtct cgtcaagatc
      361 tacaagggcc gtaagtccga ggttcgcgcc ctcgacggcg tcgacctggc cgtgcccgag
      421 ggaaccgtcc tcggcctgct cggcccgaac ggggccggca agacgacggc ggtccggatc
      481 ctggcgacgc tcctgaagcc ggacgccggc cacgcgaccg tggccggcca cgacgtcgtg
      541 cgcgagcccg acgcggtccg ccgggtgatg ggcctgtcgg gacagtacgc ggccgtcgac
      601 gagaacctga ccggcaatga gaacctctgg ctgttcggcc ggctgtaccg gctgtcctcg
      661 cccgaggcgc accgacgagc aaacgagctg ctcggcgact tcgacctgct cgatgccggc
      721 gatcgcgtcg tcaagaccta ttccggcggc atgcggcggc ggctcgatct cgccagcgcc
      781 ctgatcggcc ggccccggct gctgatcctc gacgaaccga cgaccggcct cgacccgcgc
      841 agccggctcg ggatgtggga cgtcctgcgc ggcctcgtcc gggacggtgt gacgctgctc
      901 ctgacgaccc agtacctcga ggaagccgac gagctggccg acacgatcgc ggtcgtcgac
      961 cacggcaaga tcattgcccg gggcaccgcc gacgagctga agtcgcaggt cggtggcgag
     1021 cggatcgagg tcgtcgtccg ggatcgggag tcgatcgccg cggcccggga gctgctcgcc
     1081 cgcgatgccg gcggcgacac gacgctcgac gagcacaccc gcaagttgac cgtgccggcg
     1141 cacggcgggg cgcagacgct cgtccaggtc gtccgcgacc tcgacgaggc gggcatccac
     1201 atcgacgaca tcgccctgcg gcggccgacg ctggacgacg tcttcctggc gttgaccgga
     1261 cacgcctcgg ccgaggactc gaccacggac gctccgacca gcgggagggc agcatgacga
     1321 cgacgaccgc agaccgccgt cccggtgccg tccgggctgc ggtcaccgac ggcctcctcg
     1381 tcgggtggcg caacctcaag cgcattccgc ggatcccgga gctggcgatc ttcgccgtcc
     1441 tccagtcgat catgttcgtg ctgctgttcg cgttcgtgtt cggcggcgcg atcccgatcc
     1501 cgggcggcgg cgactacaag gcgtacctga tgcccgggat cttcgcccag accatcgtgt
     1561 tcgccgcggg cacgaccgcg atcggcatgg ccgacgacgt ccacaagggg atcatcgacc
     1621 ggttccgctc gctgccgatg tcgcgctcgg cggtcctgac cgggcggacc ttctccgacg
     1681 tgatctacaa cgcgatgatc ctcgtcgtcc tgatggtcac gggcttcgtc atcggctggc
     1741 gggtcgagtc gatcccggcc ctcgtcgcgg gcttcctgct cctgctgttc ttcgcctacg
     1801 ggatggcctg gctcggcgtg tggctcgggc tcaacgtccc gacggtcgag gtcggccagc
     1861 aggtgatctt cacgaccctg ttcccgatca cgttcatctc gaacgtgttc gtgccgctcc
     1921 aggggctgcc ttcgtggctc cagccggtcg ccgaatggaa tccgacctcg accctgacgg
     1981 cggccctccg ccagctgtgg ggcaacccga acccggccac ctcgaacagc ctcgccgccc
     2041 agcagccgct cctcgtcacg atcggctggg tcgtcgtgat catcgcgatc ttcggcccac
     2101 tcggcgtccg ccgctaccgc tcgatgagtc gctgacatgg acgacgagga gctccgtccc
     2161 gagctgcggc gatcgcgccg ggcgccggca cccggttcgc gggccgccga tgccgacgcc
     2221 gccgggccgg tccccgatgg accgatcgtg gcggccaatc aggccgcgcc gggtccggcc
     2281 aacgcccgct ggcaggcgct cctggccgag ggcgggccga tcctcgccga cggcgcgatg
     2341 ggcacgatgc
//
LOCUS       DATAIW010000484         6197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_291520, whole genome shotgun sequence.
ACCESSION   DATAIW010000484 DATAIW010000000
VERSION     DATAIW010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291520"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VHM48_14325"
     CDS             <1..493
                     /locus_tag="VHM48_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756641.1"
                     /translation="VVGGQDPVLIRPILTRVVAEKRRRWPGSVLEVEVEPGLPPVTGD
                     DASVELVLRNLISNALKYGPGGGLVTIAVRRAGDAVEVRVCDEGAGIDAAASTRLFDL
                     FYRTDEARRQAQGAGIGLFVVRALVESVGGRVWAINRPDGGAEFGFGLPVFVEADDGP
                     ALD"
     gene            510..1148
                     /locus_tag="VHM48_14330"
     CDS             510..1148
                     /locus_tag="VHM48_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2756642.1"
                     /translation="MERISRVVGPAATNVHILGDERSREAIAIDTAIPSLAWIADELA
                     ARDWTLKLIVSTHGHWDHVGDNAAVAAATGAEIAVHALDRDSLAHPMPLWAPFEIPPS
                     VAAVDLAEGGEIRFGRIRLRVLHTPGHTPGSVCLQSTDDGLLFSGDTLFAGGWGRVDL
                     PGGSAEAIVESLGRLAELEPIVAVLPGHGPATTIGQERPWLELVRSSRRLPA"
     gene            complement(1233..2240)
                     /locus_tag="VHM48_14335"
     CDS             complement(1233..2240)
                     /locus_tag="VHM48_14335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756643.1"
                     /translation="MIDTWVQLPDAPAIPGLRFRMFDPARDYEAFVELVGAANLADGV
                     DYLPTVEGLRSDHEHGDEYDPRRDLILAEVEGDLAAAAETSVRTRDGIGVHQVEGWVR
                     SAWRRRGLGRALLHWTERRAAEVARVDGRSSDRALSSWPDEDQVGATTLYASEGYEIV
                     RYGFLMVRDLADPIADVALPMGLEIRPVVEADHRRIWDADTEAFRDHWNAVERTEADF
                     DSWFAEPDLDTSLWRVAWDGDEVAGSVMTFVWPKENEVLDMRRGWFEHISVRRPWRRQ
                     GLASALIADSLRGLEAAAMTEAALGVDAENVSGALRVYEAMGFHRTRIGVSYRKAFTS
                     D"
     gene            complement(2323..3426)
                     /gene="rsgA"
                     /locus_tag="VHM48_14340"
     CDS             complement(2323..3426)
                     /gene="rsgA"
                     /locus_tag="VHM48_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX2756644.1"
                     /translation="MSDRTDAPALAAFGWTPELAAAHERHSALGREPARVVAEDRASY
                     QVVGTAGELRAAVSGRFRHEANADPSAFPAVGDWVAVETRATGETVIHAVLPRRSALV
                     RQAAGKRTEAQVVGANLDVVFVVASLNPDLNMRRLERYVAAAWESGAEPVILLSKADL
                     GGDVDAVLVAVAGVAAGATIVIVSSFDGRGVEEVRERIGVGQTVAFVGSSGVGKSTLL
                     NRLAGEERALVREIREEDGRGRHTTTRRQLHLLPDGGLVLDTPGMRELSLWDGDGLEQ
                     TFTDVDEIVARCRWSNCSHNGDDGCAIAAALADGSLDRERHESWQKLLREAARHERRI
                     DALARAEERRKWKAIGKSVTKHMNAKYGQDGWR"
     gene            complement(3427..4230)
                     /locus_tag="VHM48_14345"
     CDS             complement(3427..4230)
                     /locus_tag="VHM48_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756645.1"
                     /translation="MMTMGHELKASYAFIERNFNLTRRYWGWELAFLVYSVAGALSIS
                     LIGADQGSPRLILLLMVGAIFWNYLSVVFSWIAETISVERWEGTLEYTMMAPIRRSSQ
                     LLGSVLYAMVYGLIHTAAILVVLVLFFPQLDVHGANPVTVVAFMLLGSFSFIGIGTIA
                     AILPLLYVERGAQMTFVLQSCLLLVSGVYYSIDVLPGWMQVLSHLSPATYVLDGVRKG
                     LIEGTSVTDLAGDVWPLIVMGAFLIPFGLWAFGRAEHYAKRTGKLKRVG"
     gene            complement(4266..4457)
                     /locus_tag="VHM48_14350"
     CDS             complement(4266..4457)
                     /locus_tag="VHM48_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756646.1"
                     /translation="MYSAPNDQLRLINAIRAEQIEQAAVSRRFETPAPSIRRAVGTRI
                     VRFGARLAGEPTYGLARSR"
     gene            complement(4475..5365)
                     /locus_tag="VHM48_14355"
     CDS             complement(4475..5365)
                     /locus_tag="VHM48_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756647.1"
                     /translation="MTATLIRQQKIARTDGLIGAVRTVDQAVPALLVEKVTKRFVVGR
                     KRKPVTAIANVSLRLERGDIVGILGANGSGKSTLIRLVSGLLTLDEGRVEVFGHDIER
                     DEMAVKRLINRVSVDAAFFKKLSPMENLLFAARLYGLDAAGARKQAVEILGRLGIGEK
                     RVGRPIEQMSRGMQQKVAIARALLTSPSLLLLDEPTTGLDPRSKLDVQTFIEELRETH
                     DASIVLTTHDLAEAERLCGSITVLAGGRVVVEDTPAGLIAAEAERTGRAATLHDVFMT
                     HTGRSLDDDVEDESGDDDSE"
BASE COUNT          898 a         2156 c         2185 g          958 t
ORIGIN      
        1 cgtcgtcggt ggccaggacc cggtcctgat ccggccgatc ctgacccggg tcgtcgcgga
       61 gaagcggcgg cgctggccgg gctccgtcct ggaggtcgag gtcgagccgg gtctgccgcc
      121 ggtcaccggc gacgatgcct cggtcgagct cgtcctccgg aatctcattt ccaacgcgtt
      181 gaagtacggc ccgggcggcg gtctggtcac gatcgccgtg cgacgcgccg gcgacgcggt
      241 cgaggtccgg gtctgcgacg agggcgcggg catcgatgcg gcggcatcga cgcgcttgtt
      301 cgatctcttc taccggaccg atgaggcgcg gcgacaggct cagggggccg gcatcgggct
      361 cttcgtcgtg cgggccctcg tcgagtcggt cggtggccgg gtctgggcga tcaatcggcc
      421 cgatggtggc gccgaattcg ggttcggcct cccggtcttc gtcgaggccg acgacggccc
      481 ggcgctcgac tgagctcggc gttcgcacga tggagcggat ctcgcgggtc gtcggtccgg
      541 ccgccacgaa cgtccacatc ctcggtgacg agcggtcccg cgaggcgatc gccatcgaca
      601 ccgcgatccc gtcgctcgcc tggatcgccg acgagttggc tgcccgcgac tggaccctca
      661 agctgatcgt ctcgacccac ggccactggg atcacgtcgg cgacaacgcc gcggttgccg
      721 ccgcgaccgg cgccgagatc gccgtccacg ccctcgatcg cgattcgctt gcccacccca
      781 tgccgctgtg ggctcccttc gagatcccgc cgtcagtggc ggccgtcgac ctcgcggagg
      841 gcggtgagat ccgcttcggg cggatccggc tgcgagtgct ccacaccccc ggtcacacgc
      901 cagggtcggt ctgcctccag tcgaccgacg acgggctgct cttcagcggt gacaccctct
      961 tcgccggcgg ttggggtcgg gtcgacctgc ccggcggctc ggccgaggcg attgtcgaat
     1021 cgctcgggcg gctggcggag ctcgaaccga tcgtggcggt cctgccgggc cacggccccg
     1081 cgacgacgat cgggcaggag cggccatggc tggagctggt ccggagcagc cgccggctgc
     1141 ccgcctgacc gaacggctcc gccagcccgg cggaccaggc agccccggag cggatggtcc
     1201 agaacgatgc cgtcactcac cgcccgatcg ggtcagtcgc tcgtgaaggc cttccggtac
     1261 gagacgccga tccgcgtccg gtggaagccc attgcctcgt agacccggag cgctcccgac
     1321 acgttctcgg cgtcgacgcc gagagcggcc tccgtcatcg ctgcggcctc gaggccgcgc
     1381 agggagtcgg cgatcagggc agacgcgagg ccttgacggc gccacggccg acggacactg
     1441 atgtgctcga accagccgcg acgcatgtcg aggacctcgt tctccttcgg ccagacgaac
     1501 gtcatcaccg atcccgcaac ttcgtcgccg tcccaggcca cccgccacag gctcgtgtcg
     1561 aggtccggct cggcgaacca cgagtcgaag tccgcctccg tccgctcaac cgcattccag
     1621 tggtcgcgga aggcttccgt gtcggcgtcc cagatccggc gatggtcggc ctcgacgacc
     1681 ggccggatct ccaggcccat tggaagcgcc acgtcggcga tcgggtcggc caggtcgcgc
     1741 accatcagga agccgtagcg gacgatctcg tagccctcgc tcgcgtacag ggtcgtcgcc
     1801 ccgacctggt cctcgtcggg ccaggagctc aatgcgcgat cgctcgaccg gccgtcgacc
     1861 cgcgcaacct cggcggcgcg tcgctcggtc cagtggagga gcgcccggcc gagcccgcgg
     1921 cgacgccagg cggaccggac ccaaccctcg acctggtgca cgccgatccc gtcgcgggtc
     1981 cggacgctcg tctccgccgc ggccgcgagg tcgccttcga cctcggccag gatgaggtcg
     2041 cgacgtgggt cgtactcgtc gccgtgctcg tgatcggagc ggaggccctc gaccgtgggc
     2101 aggtagtcga cgccgtccgc caggttcgcc gcgccgacga gctcgacgaa cgcctcgtaa
     2161 tcgcgggccg ggtcgaacat ccggaatcgc aggccgggga ttgcgggagc gtcggggagc
     2221 tggacccagg tgtcgatcat cgggtcacct cgtcgggttc gttcgtggtc ggtggcggca
     2281 tgggcttgcc gacgaacgtc gccgaggacg cgttcgtggc tgtcatcgcc agccatcctg
     2341 tccgtacttg gcattcatgt gtttcgtgac cgacttcccg atcgctttcc acttccggcg
     2401 ctcctccgcc cgggccaggg cgtcgatccg gcgctcgtga cgggccgctt cccgcaggag
     2461 cttctgccag ctctcgtggc gctcacgatc gaggctgcca tcggccaggg cggccgcgat
     2521 cgcgcaaccg tcgtcgccgt tgtggctgca gttgctccag cggcaacggg cgacgatctc
     2581 gtcgacgtcc gtgaacgtct gctcgaggcc gtcgccgtcc cacagcgaca gctcgcgcat
     2641 ccccggcgtg tcgaggacca gcccgccgtc cggcaggaga tggagctggc ggcgcgtcgt
     2701 cgtgtggcga ccgcggccgt cttcctcgcg gatctcccgg acgagcgctc gctcctcgcc
     2761 ggcgagccgg ttgaggaggg tcgatttccc gacgcccgag gaaccgacga atgcgaccgt
     2821 ctggccgacg ccgatccgct cccggacctc ctcgacgccg cggccgtcga aggagctgac
     2881 gatgacgatc gtggcgcctg cggcgacacc cgcgacggcg acgaggacgg cgtcgacatc
     2941 gccaccgaga tccgccttgg acaacaggat gaccggctcc gcgccgctct cccaggctgc
     3001 cgcgacatag cgctcgaggc ggcgcatgtt gagatcgggg ttgagcgagg cgaccacgaa
     3061 gacgacgtcg aggttggcgc cgacgacctg ggcctcggtc cgcttgccgg ccgcctggcg
     3121 gacgagcgcc gagcggcgcg gcaggacggc gtggatgacg gtctcgccgg tcgccctcgt
     3181 ctcgaccgcc acccagtcgc cgacggctgg aaacgcgctc ggatcggcgt ttgcctcgtg
     3241 ccggaagcgc ccgctgacgg ctgcccggag ctcgccggcc gtgcctacga cctggtacga
     3301 cgcgcggtct tcggccacga cccgggcggg ctcgcgcccg agtgccgagt ggcgctcgtg
     3361 ggcggcggcc agctcaggtg tccagccaaa cgccgcgagc gccggcgcgt cggttcgatc
     3421 gctcattcag ccgacccgct tcagcttgcc ggtccgcttc gcgtagtgct cggcccggcc
     3481 gaacgcccac agcccgaacg ggatgaggaa cgcgcccatc acgatcagcg gccagacgtc
     3541 accggcgagg tccgtcaccg atgtcccctc gatcaggccc ttgcggaccc cgtcgaggac
     3601 gtacgtggcc ggcgacagat gcgagagcac ctgcatccag cccggcagca cgtcgatcga
     3661 gtagtacacg ccgctgacga gcagcaggca cgactgcagg acgaacgtca tctgcgcccc
     3721 gcgctcgacg tacagcagcg gcaggatcgc ggcgatcgtg ccgatcccga tgaagctgaa
     3781 cgagccgagg agcatgaacg cgaccacggt gacggggttc gccccgtgga cgtcgagctg
     3841 ggggaagaac aggaccagca cgacgaggat cgcggccgtg tggatcaggc cgtagaccat
     3901 cgcgtacagg accgagccga gcagctgcga cgaacgccgg atcggggcca tcatcgtgta
     3961 ctcgagcgtg ccctcccagc gctcgacgct gatcgtctcg gcgatccagc tgaacaccac
     4021 cgagaggtag ttccagaaga tcgccccgac catcagcagg aggatgagac gcgggctgcc
     4081 ctggtcggcc ccgatcagcg agatgctgag cgcgcccgcc accgaataga ccaggaacgc
     4141 gagctcccag ccccagtagc ggcgcgtcag gttgaagttg cgctcgatga aggcgtagga
     4201 cgccttgagc tcgtgaccca tggtcatcat cggggttctc cgggtggggg ccgtggcccg
     4261 atcgctcatc gcgatcgggc caggccatag gtcggctcgc ccgccaggcg ggcgccgaag
     4321 cggacgatcc gggtgccgac ggcacgtcgg atcgacggcg cgggggtctc gaaccggcgg
     4381 ctgacggccg cctgctcgat ctgttccgcg cggatcgcgt tgatcagtcg aagctgatcg
     4441 ttcggagcgc tgtacatctc gttctcacct cctttcattc ggagtcgtcg tcgccggact
     4501 cgtcctcgac gtcgtcgtcg agcgagcggc cggtgtgggt catgaagacg tcgtggaggg
     4561 tcgccgcgcg accggttcgt tcggcttcgg ccgcgatcag cccggccggg gtgtcctcga
     4621 cgacgacgcg gccgccggca aggaccgtga tcgagccgca cagccgctcg gcctcggcca
     4681 ggtcgtgcgt cgtgagcacg atcgacgcgt cgtgcgtctc ccggagctcc tcgatgaacg
     4741 tctggacgtc gagcttcgaa cgtggatcga gaccggtcgt tggctcgtcg agcaggagga
     4801 gcgagggact cgtcaggagg gcgcgagcga tggcgacctt ctgctgcatc ccgcgactca
     4861 tctgctcgat cggccggccg acgcgcttct cgccgatccc gagccggccg aggatctcga
     4921 ccgcctgctt cctcgccccc gccgcgtcca ggccgtagag ccgggccgcg aacaggaggt
     4981 tctccatcgg gctgagcttc ttgaagaagg cggcgtcgac gctgacccgg ttgatcagcc
     5041 gcttgaccgc catctcgtcg cgctcgatgt cgtggccgaa gacctcgacc cggccttcat
     5101 cgagggtcag gagacccgag acgaggcgga tgagggtcga cttgcccgag ccgttggcgc
     5161 cgaggatccc gacgatgtcg ccgcgctcga ggcggagcga cacgttcgcg atcgccgtca
     5221 ccggcttgcg cttgcggccg acgacgaagc gcttcgtgac cttctcgacg aggagagcgg
     5281 gcacggcctg gtcgacggtt cgcacggccc cgatgaggcc gtcggtccga gcgatcttct
     5341 gttgtcggat cagggttgcg gtcacggctc gtgactcctg ggctggtgcg cggatggtgg
     5401 actgggtgct gctggagccg ggtcggaatc gaacgagccg cggatccagt tcgacgcttc
     5461 cggcgcatcg ggtgggtcca cggctcgggg gatgcccggt ggggcatcaa aagagccggg
     5521 ggcccgtctg gcccacggct cgtggttcgc gcttccggcg gttcgaccgc ctcaggctac
     5581 ctcgagatgg gcacagcact cccgccgatg acggcgccgt cgggcgcggt cgaaggccgg
     5641 aaccggaggt gcgtcatgga gcaattcatc tggcacgcct ccctggctgc gcaagtcgag
     5701 tggcgggcgg tcgccgcgcc gatcggcagt ctaggggtcc ggcgccgcgc tcgtcaaccc
     5761 cgtcggatga cggttcggac acgcgacaca tgacgtaggg ccaagcccgt gacgcccagc
     5821 ggcgcctcgc gtgccgtctt cgcgcatctg atgcgccaat tgcggcattg cgttcatcag
     5881 atgaacctac gcgacggact ggacctcccc gggtgtcgac tcactcccga atcggccgga
     5941 ttcggtccga gtccgtgcat ctgatgcacc agatcggacg ctcgatggcc ccgaaggctc
     6001 caggcacgtc ggataggttc atcggacgca catcggcctg ggctcaggcg tcgcggcgga
     6061 cgatgaaccg gcggaccgtc gcctgcggcg aatcgatctc gtggacgacc cggaacccgg
     6121 ccgccaggaa ggtcgacagg tggccggtgt agccgagggc agcgggaacc cggccgtcgc
     6181 ccgggtcgac ggggtac
//
LOCUS       DATAIW010000485         6355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_201529, whole genome shotgun sequence.
ACCESSION   DATAIW010000485 DATAIW010000000
VERSION     DATAIW010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201529"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..611
                     /locus_tag="VHM48_14360"
     CDS             183..611
                     /locus_tag="VHM48_14360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2756648.1"
                     /translation="MGSYPATVHLVSGDGTERQEPFDLRVAYRIGAVELRLGQLSGGE
                     ALHFALAMCVFNNIARESRNRGIQLRELSVTADGDFDSDELSTGIAFSISIAGAATEE
                     VLRDLAQFGADDSTIAKTLRHPTSVTLTAIDATAATEPVA"
     gene            complement(780..2156)
                     /locus_tag="VHM48_14365"
     CDS             complement(780..2156)
                     /locus_tag="VHM48_14365"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2756649.1"
                     /translation="MIHVPVPGLVVLIGAAGAGKTTLAIRLFEPAEILSSDALREALS
                     GDAADQRPTRTAFRILHREARRRLDAGRLVIVDATNVDTRARRSLLEIAAATDAPTLA
                     IVLALAAAEVHLQNARRHGRVVPADIVDRHLDAAATLGADPASIAAALRAGGFASVIV
                     ATSAAEVAALRVVRIIGWRPRPVTPLDPEVSALLGQLVPAIQARLGSDLVAVYLCGSA
                     VTGGYDPGISDVDLLVVVEGDPERLDLERLAAMHDAIARRDPAWRDRIETVYVGRSTL
                     QAFRTHPGRLAVISPGEPLHLRDDRASQWVQNWFYARVVSLTLRGPAPDTLIPPIDWS
                     EFVSAITRYADELARRPLADADPGSIAYVVLTMCRAAMTIRVGGLGSKADAAAWAGAM
                     LPDRSELIDMALACRRSGGMIGFQDEGSVTGARTLIAELQTLIRSDPSVSVGDRPPVG
                     VKLPATSG"
     gene            complement(2222..2788)
                     /locus_tag="VHM48_14370"
     CDS             complement(2222..2788)
                     /locus_tag="VHM48_14370"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2756650.1"
                     /translation="MSHLSTTLRIEASATAVFDLIADPARSPEWQTLLAEMGDIAGRP
                     GGIGSSFVGFFRVAGRKLAGRFIVTAAERPTVFQVNETTTGGWARWTTMIEEAGAGCD
                     IAVKLEYELPGEIVGSLFGLLTGNRLEREFRRTYDNLKRLAETGTAASLASSGPSRFV
                     QPASSSRAGANEAHDDDAGDAEGRLAAT"
     gene            complement(2913..3776)
                     /locus_tag="VHM48_14375"
     CDS             complement(2913..3776)
                     /locus_tag="VHM48_14375"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2756651.1"
                     /translation="MLGRYVTRAVDAQARWSKPLGDFNHRWLAALFHPIRPIQNFLNG
                     SWLGHPVHAVVTDVPIGAMTVSIIADVIGQPVVADVSLLLGVLAMVASAVTGAADYTE
                     VDGNARNRATVHSVVMVLSLVVYAASLLIRSGSPSDRLVPILLALAGYLLLMLGAAIG
                     GDLVYLIGTHVNRHAWRGAGTKWIKLDLGGLDDIPEGGPTKLKAGINDLAVIREGDRI
                     LAVHAQCAHAGGPLAEGSIVAGAIQCPWHGSRFRLIDGHNVRGPAMYDQPAYEVRRAE
                     AGGWEVLRRPS"
     gene            3855..5141
                     /locus_tag="VHM48_14380"
     CDS             3855..5141
                     /locus_tag="VHM48_14380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756652.1"
                     /translation="MPANHHDAEARDRDGRSDWKRIVAVFWLTSMVEGLGVSQVFSFL
                     APYLSIVGVPDADRAPFVGLFSALIFVVGMPLVPLWGVWADKYSRKAVVVRSCLVEAV
                     VFVCVALSREPWQLAVSLLLIGFQLGNTGIMLGAIRDVVPRRRIGSVIALFGASGPVG
                     FAVGPTLGGYLVDGLGWPLPSMFWVSAVLSIATAAVVWFGSREVRPAFVPEGRVLPLA
                     YGALRSVLGDPTVRRIFAIYGVAFLANQMSRPYQALIVESIVGKGAGLASSIGFVAGT
                     AALVGAAIAPLGGVVGDRIGFRPVLLVSLFVGGFALLAVPLIGAIPPLAVAILVFTAA
                     NGLVGAMIFSLLATEVPAERRSQTLNLVYLPLYAAGIIGPLVGGAVSAVAGPTGPFWA
                     GAAVFLVGAVVVALRVRTHAVPPDAPVEPHAPGTLA"
     gene            5253..6146
                     /locus_tag="VHM48_14385"
     CDS             5253..6146
                     /locus_tag="VHM48_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX2756653.1"
                     /translation="MALNVPPDREVALHEQLEETDRSLVYDLSTLVDRRQVLRLAGLT
                     TISAGLMSIVACAPSASGSASPSRASSAAGSGSSASAAAAADCAVIPEETAGPFPGDG
                     SNGPNVLAQSGIVREDIRSSFGTSTTKATGVPLTIKLTIQDAANNCAPMAGAAVYLWH
                     CDQAGNYSLYSQAAASENYLRGVQATDGSGVVTFQSIFPACYSGRWPHIHFEVYTDLE
                     AATDEANKIATSQIALPKEACALVYATGGYSQSVSNLSRVSLQSDMVFGDDGGVHELG
                     TITGDVSNGMTVALTVPVRAA"
BASE COUNT          940 a         2224 c         2195 g          996 t
ORIGIN      
        1 gtctatctcc gcaacggcgt ctgggtggag ttgcgggccc gctcgttcgg gcgggtcagg
       61 gacgactgag ggcctgtgaa tgaagcaggc ccttgactta tcctcccaca agacggagac
      121 agaaccgagg cccgatcggg accgctcggc ccgggctgcg gaactcgcga gggagagctg
      181 agatgggttc gtatccggcg accgtgcacc tcgtctccgg cgacgggacc gagcggcagg
      241 aacccttcga cctccgggtc gcctaccgga tcggcgccgt agagctgcga ctcgggcagc
      301 tctcgggcgg cgaggcactt cacttcgccc tggcgatgtg cgtcttcaac aacatcgccc
      361 gcgagagccg caaccgggga atccagctcc gcgaactgtc cgtcaccgcc gacggcgact
      421 tcgactccga cgagttgtcc acgggaatcg cgttctcgat ctcgatcgcc ggcgccgcca
      481 ccgaggaggt gctgcgcgac ctcgcccagt tcggtgccga cgactcgacg atcgccaaga
      541 cgctgcggca tccgacctcg gtcacactga ccgccatcga cgcgacggcg gccaccgaac
      601 ccgtcgccta actcggctcg gcctcgttcg agccgttgag gcgggcgttc acgagccgga
      661 cctctgtccc acccccgaat cgagttcggg atctccgcga tccgatcaac cggcacaggc
      721 tctgagttcg gcagcaaggt gagattccgg ttggtgggta aggctctcgc ccacctggat
      781 cagcccgatg tcgcgggcag cttcacgcca acgggcggcc ggtccccgac cgagacgctc
      841 gggtctgatc ggatcagggt ctgcagctcg gcgatcaatg tccgggcccc agtgacgctc
      901 ccctcgtcct ggaagccgat catcccgccg gaccggcggc aggccaatgc catgtcgatc
      961 aactccgacc ggtcggggag catcgctccc gcccacgccg cggcgtcggc cttcgagccc
     1021 aggccgccca cccgtatggt catggcagca cggcacatcg tgagaacgac gtacgcgatg
     1081 gatcccggat cagcgtcggc gagcggtcgc cgcgccagct cgtcggcgta ccgcgtgatc
     1141 gcgctcacga actcgctcca atcgatgggc gggatgaggg tgtcgggggc ggggcctcgc
     1201 agcgtcaggg acacaacccg cgcgtagaac cagttctgga cccactggct cgcccgatcg
     1261 tcgcggaggt ggaggggttc acccgggctg atgacggcga ggcggccggg atgcgtccgg
     1321 aaggcctgga gcgtggatcg accgacatag acggtttcga tgcggtcgcg ccacgcggga
     1381 tcgcgccggg cgatcgcgtc atgcattgct gcgagcctct ccaggtccag ccgctcaggg
     1441 tcgccttcga cgaccacgag cagatcgaca tcgctgatcc ccgggtcgta accgccggtg
     1501 accgccgagc cgcagagata caccgcgacg aggtctgatc cgagcctggc ctggatcgcc
     1561 gggaccagct ggccaaggag agccgaaacc tcggggtcga gcggcgtcac cggtctcggc
     1621 ctccagccga tgatcctgac cacacgaagc gcggcaacct ccgctgccga ggtggcgacg
     1681 atgaccgacg caaatcctcc ggctcggaga gcggctgcga tcgaagctgg atcggcgccg
     1741 agcgtcgcgg ccgcgtcgag gtgtcgatcg acgatgtcag cagggacgac ccggccatgg
     1801 cgtcgagcgt tctggaggtg cacctcggcc gccgccaacg caaggacgat cgccagcgtg
     1861 ggcgcatcgg tcgcagctgc aatctcgaga agcgatcgtc gtgcgcgggt gtccacgttc
     1921 gtcgcgtcga cgatgacaag gcgcccggcg tcgagtcggc gacgggcctc gcggtggagg
     1981 atccggaacg ccgtccgtgt cggccgctga tcggcggcgt cgccgctcag cgcctcgcga
     2041 agggcgtccg acgacaggat ctccgccggc tcgaacaggc ggatcgccag cgtggtcttg
     2101 ccggctcccg cggcgccgat cagaacgacc agaccgggca ccgggacatg gatcacgccg
     2161 gcgatcaccg gatcagcacc gaatcaacac tgagccggcc gtcggtcgcc tccggaatcg
     2221 gtcaggtggc ggcgagccgc ccctcggcgt cgccggcgtc atcgtcgtgg gcttcgttgg
     2281 cgccggcgcg cgacgatgac gccggctgga cgaacctcga cggcccggag ctcgcgaggg
     2341 atgccgccgt ccccgtttct gcgagtcgct tgaggttgtc gtacgtgcgg cgaaactctc
     2401 gctccaggcg gttgcccgtc agcaggccga acagggagcc gacgatctcg ccgggcaact
     2461 cgtattccag cttgaccgcg atgtcgcatc cggctccggc ctcctcgatc atcgtggtcc
     2521 agcgggccca gccgccggtc gtcgtctcat tgacctggaa gaccgtcggg cgttcggccg
     2581 cggtcacgat gaaacgaccc gcgagcttgc ggccggcgac ccggaagaag ccgacgaagc
     2641 tcgacccgat gccaccggga cgcccggcaa tgtcgcccat ctccgcgagc agggtctgcc
     2701 actcggggct ccgtgccggg tcggcgatca gatcgaagac cgctgtcgcg gacgcctcga
     2761 tccggagcgt ggtcgaaagg tggctcatgc gttcccccgc cagatccctg cgcgcaagat
     2821 cgtccgaagc gtccgatccg gatgctacgc ccccggccgt cgggcgtcat tacggatgat
     2881 tcgatattcg cacctggccg gtcgttcccc cgtcagctcg gccggcgcag gacctcccag
     2941 ccgcccgctt ccgcccggcg gacctcgtag gccggctggt cgtacatcgc cggtccgcgg
     3001 acgttgtgac cgtcgatgag ccggaaccgt gagccatgcc acgggcactg gatcgctccg
     3061 gcgacgatgc taccctcggc cagtggcccg ccggcgtggg cgcactgggc atggacggcg
     3121 aggatgcgat ccccctcgcg gatcactgcg aggtcgttga tcccggcctt gagcttcgtc
     3181 ggtccgccct cggggatgtc gtcgaggccg ccgagatcga gcttgatcca tttcgtcccg
     3241 gcgccgcgcc aggcgtggcg gttgacgtgg gtcccgatga ggtagacgag gtcgcccccg
     3301 atcgcggcgc cgagcatcag caggagatag ccggcgaggg ccagaaggat cgggacgaga
     3361 cggtccgacg ggctgccgct ccggatcagg agcgacgccg cgtacacgac cagcgagagg
     3421 accatgacga ccgagtggac cgtggcccgg tttcgcgcgt tcccgtcgac ctcggtgtag
     3481 tccgcagcgc ccgtgaccgc cgaggcgacc atcgcaagga cgccgaggag gagcgagaca
     3541 tcggcgacga cgggctggcc gatgacgtcg gcgatgatcg agaccgtcat cgccccgatc
     3601 ggcacatcgg tgacgacggc gtgcacggga tggccaagcc acgagccgtt gaggaagttc
     3661 tggatcggcc ggatggggtg gaacagtgcc gccagccagc ggtggttgaa atcgccgagt
     3721 ggcttcgacc agcgcgcctg ggcatcgacc gcgcgggtca cgtagcgacc aagcatcctc
     3781 gtcgacctcc ttcaaccgag tctacgccgg gactcccggc gccggatctg gcggacccgg
     3841 taggcttcgc ggccatgcca gccaaccacc acgacgcgga ggcgcgcgat cgcgacggcc
     3901 gctccgactg gaagcgaatc gtcgccgtct tctggctcac ctcgatggtc gagggcctgg
     3961 gcgtcagcca ggtcttctcg ttcctggcgc cgtacctgtc gatcgtcggc gtgcccgacg
     4021 cggatcgggc gccgttcgtc gggttgttct cggccctgat cttcgtcgtc gggatgccgc
     4081 tggtcccgct ctggggtgtc tgggccgaca agtacagccg caaggccgtc gtcgtccgga
     4141 gctgcctcgt cgaggccgtg gtgttcgtct gtgtcgccct gtcgcgggag ccgtggcagc
     4201 tggcggtgag cctgctgctg atcgggttcc agctcggcaa taccgggatc atgctcggcg
     4261 cgatccgcga cgtcgtgcca cgtcgtcgga tcgggtcggt catcgccctg ttcggcgcgt
     4321 cgggtccggt cggcttcgcg gtcgggccga ccctcggcgg ctatctcgtc gacgggctcg
     4381 gctggccgct gccgtcgatg ttctgggtgt cggccgtcct gtcgatcgct accgcggcgg
     4441 tcgtctggtt cggctcacgg gaggtccggc cggcgttcgt gcccgagggc cgcgtcctgc
     4501 cgctggccta cggcgcgctg cggagcgtcc tcggcgaccc gaccgtccgg cggatcttcg
     4561 cgatctacgg cgtggccttc ctcgccaacc agatgagccg gccctaccag gcgctgatcg
     4621 tcgaatcgat cgtcggcaag ggagcgggcc tcgcgtcgtc gatcgggttc gtcgccggca
     4681 ccgcggctct ggtcggggcg gcgatcgcac cgctgggcgg tgtcgtcggc gatcggatcg
     4741 ggttccggcc cgtcctgctc gtctcgctgt tcgtcggcgg gttcgcgctg ctcgcggttc
     4801 ccctgatcgg cgcgatcccg ccgctggcgg ttgcgatcct ggtcttcacc gccgccaacg
     4861 gcctggtcgg agccatgatc ttcagcctgc tcgcgaccga ggtgccggcc gagcgccgct
     4921 cgcagacgct gaacctggtc tacctgccgc tctacgccgc ggggatcatc gggcccctcg
     4981 tcggcggtgc ggtctcggcc gtcgcgggcc cgaccggccc gttctgggcg ggcgccgcgg
     5041 tgttcctggt cggcgccgtg gtcgtagcac tgcgcgtgcg gacccacgct gtcccgccgg
     5101 atgcgccggt ggagccccac gcgccaggca ccctggcctg acgcgcggcg tgacctcccg
     5161 tcaccgttct gcaaggtcgg cctgacgacg ctccgcggtg tcgccgcccg gaatccaggg
     5221 tgggacgctc gcacgacaag gaggcccgcc ccgtggccct gaacgttccg ccggaccgcg
     5281 aggtcgcgct ccacgagcag ctcgaggaga ccgaccgcag tctcgtgtac gacctctcga
     5341 ccctcgtcga tcgccgccag gtcctcaggc tcgctgggct gacgacgatc agcgctgggt
     5401 tgatgtccat cgtcgcctgc gccccatcgg cgagcggaag cgcgtcaccg tcacgtgcct
     5461 cgtcggcggc cggctccgga tcgagcgcct cggcagccgc ggcagcagac tgcgccgtca
     5521 tccctgagga gaccgccggc ccgtttccgg gcgacggctc gaacggcccg aatgtcctcg
     5581 cccagagcgg catcgtccgc gaggacatcc gctcgagctt cgggacctcg acgacgaagg
     5641 ccaccggcgt gcccctgacg atcaagctca cgatccagga cgccgcgaac aactgcgccc
     5701 cgatggccgg cgcggcggtc tatctctggc attgcgacca ggcaggcaac tactcgctct
     5761 attcccaggc ggcggcgagc gagaactacc tccgcggtgt ccaggcgacc gacgggtcgg
     5821 gtgtcgtgac cttccagagc atcttcccgg cctgctattc cggccgttgg ccgcacatcc
     5881 atttcgaggt ctacaccgac ctggaagccg cgaccgacga ggcgaacaag atcgcgacct
     5941 cgcagatcgc gctgccgaag gaagcctgcg cgctggtcta tgcgactggc ggctacagcc
     6001 agagcgtctc gaacctcagc cgggtctcgc tccagagcga catggtcttc ggcgacgacg
     6061 gcggcgtcca cgagctcggt acgatcaccg gcgacgtatc gaacgggatg accgtcgcac
     6121 tgaccgtgcc ggtcagggcc gcctgacggc ggcccggcgt cgtcctcacg aacacaatgg
     6181 gaactccttg gccccagcat ggcgaacgag atcgaggacg tgcccattcg acccggtgaa
     6241 caccgccgcg tcgtgggtcg agggcagcag cgcgcatccg gccacgacga gcgcaaacgc
     6301 gaatccaacg agttgcctca tcacgcctcc agagccgccg ggcgctgcga tcggg
//
LOCUS       DATAIW010000486         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_201641, whole genome shotgun sequence.
ACCESSION   DATAIW010000486 DATAIW010000000
VERSION     DATAIW010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201641"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VHM48_14390"
     CDS             <1..518
                     /locus_tag="VHM48_14390"
                     /inference="COORDINATES: protein motif:HMM:NF044559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756654.1"
                     /translation="PRDDVTGCIARLAPCTLSSVTCPASWIRTRTSTKLIDAYFADQK
                     IIAISTERLRREHVPLPKGPKPGYDGRLSGWLTEMLNYGPPDGPERAWPLILRLIDRG
                     PDVNALGWVGAGEVEDLVNRSGAAFADRIIDEAAKSRRFRAALRHVWPNEDVPNSLQT
                     LIRDSRAGDTK"
     gene            991..1671
                     /locus_tag="VHM48_14395"
     CDS             991..1671
                     /locus_tag="VHM48_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756655.1"
                     /translation="MDARILLVEDDPSIREVTAIGLGAAGFAVTAAVDGVEGLDRFRA
                     DPYDLVLLDIMLPRLDGYEVCRQIRRTSTVPVVMLTARGDTMDVVVGLEAGADDYVRK
                     PFDLPELIARIRAALRRAGGAADGATELRIGTLIVDAAGRTVTREGADVPLTRTEFDL
                     LTELVRHAGQVLSRDVLLDRVWGYDYLGDSRLVDVAIQRLRAKVEADPAAPELIQTVR
                     GAGYKAVR"
     gene            1729..>2167
                     /locus_tag="VHM48_14400"
     CDS             1729..>2167
                     /locus_tag="VHM48_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756656.1"
                     /translation="MRARLTATIVFLVVLTAIVLGVAAYVFVDTRLHQQTLEEARDQA
                     RFDLSVLAPPILGDPPTPQSIEALAAAFRFRSLESIIVPVGGLPSYDPTSLDGTIDRI
                     PASVRQYVAGGQIAYSWQTVDGVPSLIVGGRSGGTGAEIYLVHN"
BASE COUNT          331 a          790 c          715 g          331 t
ORIGIN      
        1 gtccacgcga tgacgtgaca ggatgcattg cgaggttggc gccttgtacc ctgagttcgg
       61 tgacatgccc ggcatcgtgg atccgaacac gaacctcgac gaaactgatc gatgcgtact
      121 tcgccgatca gaagatcatc gcgatcagca ctgaacggct tcggcgcgag cacgtgccgc
      181 tcccgaaggg gccgaagccc ggctatgatg gccggctgtc gggctggctg accgagatgc
      241 tcaactacgg gccgccagat gggccggaac gagcgtggcc gctcatcctg cgactcattg
      301 accgcggccc cgacgtcaac gccctgggtt gggtcggggc cggcgaagtc gaggacctcg
      361 tcaataggtc cggcgccgcg ttcgctgatc gcatcatcga tgaggcggcc aagagccgga
      421 ggttccgcgc ggcactccgc cacgtctggc ccaacgaaga cgttccgaac tcgcttcaga
      481 cactgatccg cgacagccgt gcaggcgata cgaagtagct ccggatgccg ctccgcacgc
      541 tgcccgcatg agttcagcct cgcccgtgtc aagggaagcg gttccaatca gtcggtattc
      601 gtgaccggca tttgtgcgcc aaaggagcaa cgggaagctg ggaacgctct tccgtgatcc
      661 gtctgctcgt cggacgcaca acatcgacgc caaattcgcc gttcgtggcc gggtttgacc
      721 gctacacgca caatctggcg ccgggccacg cgtttcgttg tccggttgac cgccacgcgc
      781 acaaatccgc gccgcgaccc gatggagccg gacgccaacg ggtcggcgcg cgccgcaccc
      841 cgccattgaa gccgccctcg agcccgtttg cgacagaacg gtgacccgcg acgaacccga
      901 cggccctgtc gcccggcgtc gagcgagcgg tatcctgccg ccgagcccgg tcgaacggcc
      961 ggccgcgaga gggaggcgcg agagacgccc gtggacgccc ggatcctgct cgtcgaggat
     1021 gacccgtcga tccgcgaggt gaccgcgatc gggctgggag cggccgggtt cgcggtgacg
     1081 gccgccgtcg acggtgtcga gggtctcgac cgcttccgcg ccgaccccta cgacctcgtg
     1141 ctgctcgaca tcatgctgcc gcggctcgac ggctacgagg tctgccgcca gatccgccgg
     1201 acctcgacgg tgccggtcgt catgctcacc gcccgcggcg acacgatgga cgtcgtggtc
     1261 gggctcgagg ccggcgccga cgactacgtc cgcaagccct tcgatctccc ggagctgatc
     1321 gcccggatcc gggccgcgct gcgtcgggcc ggcggggcgg ccgacggcgc caccgagctt
     1381 cggatcggca ccctgatcgt cgacgccgca ggacggacgg tcacccgcga gggggcggac
     1441 gtgcccctga cccgaaccga gttcgacctc ctcacggaac tcgtccgcca tgccggacag
     1501 gtcctctcgc gcgacgtcct cctcgatcgc gtctggggct acgactacct cggcgactcg
     1561 cgtctcgtcg acgtcgcgat ccagcgcctg cgggccaagg tcgaggcgga cccggccgcg
     1621 cccgagctca ttcagaccgt ccgcggcgcc ggctacaagg ccgtccgctg accgcggccc
     1681 cgacgcccac ccgacgcgcg ccgagccggt gacccgatga tccgcggcgt gcgggcccgc
     1741 ctgaccgcga cgatcgtctt cctcgtcgtc ctgaccgcga tcgtcctcgg cgtcgcggcc
     1801 tatgtcttcg tcgacacgag gctccaccag cagacgctcg aggaggcgcg cgaccaggct
     1861 cgcttcgacc tgtccgtcct cgccccgccg atcctcggcg atccgccgac gccgcagtcc
     1921 atcgaggccc tcgccgcggc cttccggttc cgctccctgg aatcgatcat cgtgccggtc
     1981 ggaggcctcc cctcgtacga cccgacctcg ctcgatggca cgatcgaccg aatcccggcc
     2041 tcggtccgcc agtacgtcgc cggcgggcag attgcctact cgtggcagac cgtcgacggc
     2101 gtgccgagcc tgatcgtcgg cgggcggtcg ggcgggacag gggccgagat ctatctggtc
     2161 cataacg
//
LOCUS       DATAIW010000487         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_256979, whole genome shotgun sequence.
ACCESSION   DATAIW010000487 DATAIW010000000
VERSION     DATAIW010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256979"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VHM48_14405"
     CDS             <1..1572
                     /locus_tag="VHM48_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756657.1"
                     /translation="LYRTASKLDRHPAALIPGKPIQVDVLRDSLRRIADDVLDRGFDP
                     IDGAHALRAARDLIARTRPRLRSNGGEPATTAVRLEAEDPLDAAVRLALDLDHGVLPI
                     QGPPGTGKTWTGARMIAALVEAGKKVGITAQSHRAITNLLDKTSEAFTEKHQPFAAVQ
                     KSDGEAGSARTGVRIVDDAKDVAPLLAGDTVPIAAGTVWLWARADLMNAVDVLFVDEA
                     GQLSLANTLAAAGAARSLILLGDPNQLPQVSQGIHPEGAGASALEHLVGDALTVPPDR
                     GLFLPRTYRLHPDVNGYISEIFYEGRLGTDPSTARQDLRGRLPIGGTGIRWQPVGHDG
                     DESSSIAEAERVIDAIEDLLGREWVDRDGRPRRLGIEDIVVVAPYNAHVAAIQACAER
                     RLGRLARVGTVDRFQGQEAPVAIYSMAASSVDDAPRGMEFLYDGHRLNVAVSRAMGLA
                     IVVASPDLLLVAPHGPAQMRKVNAICRLVEIAAEQAGRDGPGRLAPAPSDPTPAPSDP
                     TPARPSDAGVLTLGL"
     gene            1616..2884
                     /locus_tag="VHM48_14410"
     CDS             1616..2884
                     /locus_tag="VHM48_14410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756658.1"
                     /translation="MTIPALDLPASVLVRLRRRTHWSLVAGVALGSTGHIAASTVSTI
                     AAQQIAGSTAWSGAPGAAVVLGAAIGAVALSQLMVRSGRRVGLATGYGIGVVGAFIAT
                     LAVVASSMPMLLAGTVLIGFGNASNQLSRYVAADLYPPNRRASAIGIVVWGATVGAVI
                     GPNLAAPAASLAVSLGLPTLSGAYLVPVVFVGAAALLTFFGLRPDPYALADTSSRHDH
                     PDSQRSTPASLAAVLARPNVPVAIVALVTVQAVMVLVMTMTPLHMTSHGHDLAAVGIV
                     ISGHTLGMFGLSPISGRLTDRFGMVPVILAGMGLSAFASGLAAVAPADGGILLFIALF
                     LLGYGWNLGYVAGSALLTHGLSLAERTRLQGLADGLIWSSAAAASLGSGVVVAAASYA
                     ALGLLGATLVVVPTLLVIARRSAVAGHAAA"
BASE COUNT          369 a         1098 c         1040 g          454 t
ORIGIN      
        1 ctctaccgca ccgcgagcaa gctcgaccgc catccggcag cgctcattcc cggcaagccg
       61 atccaggtcg acgtcctgcg ggattccctg cggcggatcg ccgatgacgt gctcgaccgc
      121 ggattcgatc cgatcgacgg cgcgcacgcg ctgcgagctg cccgcgatct gatcgcgcga
      181 acccggccgc gactccgctc caatggcggc gagccggcga ccaccgcggt gcggctcgag
      241 gccgaggatc cgctcgacgc ggccgtccgg ctcgccctcg atctcgacca tggcgtcctg
      301 ccgatccagg ggccgccggg caccggcaag acgtggaccg gcgcccggat gatcgctgcc
      361 ctcgtcgagg cggggaagaa ggtcgggatc accgcccagt cgcatcgggc gatcaccaac
      421 ctgctcgaca agacctccga ggcgttcacc gagaagcacc aacccttcgc ggccgtccag
      481 aagtcggacg gcgaggccgg gtcggcgagg acgggggtgc ggatcgtcga cgacgccaag
      541 gacgtcgcgc cactcctcgc tggggacacc gttccgatcg ccgcaggcac ggtatggctt
      601 tgggcacggg ccgacctgat gaacgcggtc gacgtgctgt tcgtcgacga agccgggcag
      661 ctgagcctcg cgaacacgct cgcggcggcc ggcgcagccc gctcactgat cctgctcggc
      721 gacccgaacc agctgccgca ggtcagccag gggatccacc ccgaaggagc gggcgcatcg
      781 gcgctcgagc atctcgtcgg cgacgccctg accgtcccgc cggaccgcgg cctgttcctg
      841 cccaggacct atcgcctgca cccggacgtg aatggctaca tttccgagat cttctacgag
      901 ggccggctcg gcaccgatcc gtcgacggcc cgccaggatc tgcgtggcag gttgccgatc
      961 ggcggtaccg ggatccgctg gcagccggtc ggccacgacg gcgacgagag ctcgtcgatt
     1021 gccgaggcag agcgggtgat cgacgcgatc gaggacctgc tcggccgcga gtgggtggac
     1081 cgcgacggtc gtccgcgacg gctcgggatc gaggacatcg tggtcgtggc gccgtacaac
     1141 gcccatgtcg cggccatcca ggcctgcgcg gaacggcggc tcggccgact ggcccgggtc
     1201 ggcacggtcg atcggttcca gggtcaggag gcgcccgtcg cgatctactc gatggccgcc
     1261 tcctcggtcg acgacgcgcc gcggggcatg gagttcctgt acgacgggca tcgcctgaac
     1321 gtcgccgttt cgcgggccat gggcctcgcg atcgtcgtcg ccagtccaga cctgctgctc
     1381 gtggcgcccc acggcccggc gcagatgcgc aaggtgaacg cgatctgccg cctcgtcgag
     1441 atcgccgcgg agcaggctgg acgcgacggt cccggccgcc tcgcgccggc gccgtccgac
     1501 ccgacgccgg cgccgtccga cccgacgccg gcgcgcccga gcgacgccgg ggtcctcacg
     1561 ctcgggctgt gaacgctcga catccgacgg ggtggggcgg tcgtatcctc cgcggatgac
     1621 gatccccgcc ctggacctgc cggcctcggt cctcgttcgt ctgcgtcggc ggacgcactg
     1681 gtcgctcgtc gccggcgtcg ccctcggcag caccggccat atcgcggcgt ccaccgtgtc
     1741 gacgatcgcc gcccagcaga tcgcggggag cacggcgtgg agcggtgcgc cgggtgccgc
     1801 cgtggtcctc ggagcggcga tcggggcggt tgccctgtcc cagctcatgg tgcgatccgg
     1861 tcggcgggtc gggctcgcaa ccgggtacgg gatcggcgtg gttggagcgt tcatcgcgac
     1921 cctcgcggtc gtggcgtcct cgatgccgat gctcctcgcc gggaccgtcc tgatcggctt
     1981 cgggaacgcc tcgaaccagt tgtcgcgcta cgttgccgcc gacctgtacc cgcccaaccg
     2041 gcgcgcctcg gcgatcggga tcgtcgtctg gggcgcgacc gtcggggccg tcatcggacc
     2101 gaacctcgcc gcccccgccg cgagcctcgc ggtcagtctc gggctgccga cgctgtcggg
     2161 ggcctatctc gtcccggtgg tcttcgtcgg cgccgccgcg ctgctgacct tcttcggcct
     2221 ccgtcccgac ccgtatgccc tcgccgacac gtcgtcccgg cacgaccacc ccgacagcca
     2281 gcggtcgacg ccggcgtcgc tggccgccgt cctcgcccgg ccgaacgtgc cggtcgcgat
     2341 cgtcgcgctc gtcacggtcc aggccgtcat ggtcctggtc atgacgatga ccccgctcca
     2401 catgacctcc catggccacg atctcgccgc cgtcgggatc gtcatcagcg gccacaccct
     2461 cgggatgttc gggctgtcgc cgatctcggg ccggctgacc gaccgcttcg ggatggtccc
     2521 ggtgatcctt gccggaatgg gcctgtccgc gttcgcgtcg ggtctcgctg cggtcgcccc
     2581 ggcggacggc ggaatcctcc tcttcatcgc cctgttcctg ctcggatatg gctggaacct
     2641 cggctacgtc gccgggtcgg cgttgctgac ccacgggctc tcgctggccg agcggacccg
     2701 cctccaggga ctggccgacg ggctgatctg gagctccgcc gctgccgcca gcctcggatc
     2761 cggggtggtg gttgccgcgg cgagctacgc cgcgctcgga ttgctgggcg cgacgctcgt
     2821 cgtggttccg acgctgctgg tcatcgcccg ccgctcggcg gtggcgggtc acgcggcggc
     2881 ctgacccgaa atcgggtcgc gagagggccc ggccggagct cggcccgggc tcggcgcgat
     2941 ccgttttcac ccggtgagca c
//
LOCUS       DATAIW010000488         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_257005, whole genome shotgun sequence.
ACCESSION   DATAIW010000488 DATAIW010000000
VERSION     DATAIW010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257005"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VHM48_14415"
     CDS             complement(<1..563)
                     /locus_tag="VHM48_14415"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2756659.1"
                     /translation="MDRAEELAALTAEIVACRACPRLVAWREQVAHEKVARFRDETYW
                     GRPVPGFGDPGARILIVGLAPAAHGGNRTGRVFTGDASGDFLFAAMHAVGLANQADGR
                     RADDGLSLTGAYIAAAVRCAPPANKPTPLERATCQPFLVRELALLADVRVLVALGAIA
                     WESSFRTIAALTGDDTRPRPKFRHGAVS"
     gene            complement(574..1380)
                     /locus_tag="VHM48_14420"
     CDS             complement(574..1380)
                     /locus_tag="VHM48_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756660.1"
                     /translation="MTASSLPLSSLFTLGSRRARFLVERNIYVYRHGWIIILSGFFEP
                     LFYLGSIGFGLGALIGTLTGPGGQPISYQLFVAPALLASAAMNGAIAEGTFNFFFKLR
                     YNKTFEAILATPLSAGDVAVGELVWALIRGGIYAVAFLLVMALLGLIASPWVLLTVPA
                     ALLIGFAFGGVAMAATSFMRSWQDFDLIQLIVLPMFLFSGTFYPLDAYPEALRVVVQL
                     TPLYQGVDLLRSLSVGHLDGAMFIHVAYLGVMGIAGLWVVSRRLDKLLLK"
     gene            complement(1377..2162)
                     /locus_tag="VHM48_14425"
     CDS             complement(1377..2162)
                     /locus_tag="VHM48_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756661.1"
                     /translation="MTVMARVVEHRAMQYRKTVRASISSTFLNPVLFLLAMGVGLGTY
                     VDRSGTATTALGGLSYLQFLAPGLLAATTMQAAAFEATFPIMGGLTWQKTFHAMYATP
                     ISPRDIVVGNLVWIAIRLTAISAIFTVVMTVFGAAASPLIVLGIPAAVLTGMAFAAPI
                     AGFSATQRTPEKFNAVFRFGITPLFLFSGTFFPISNLPAAIQPIAWLSPLWHGVELSR
                     GLALGTIGANPALAMAHVAVLAAILAIGSVWAFRTVEARLVRG"
     gene            complement(2167..>3098)
                     /locus_tag="VHM48_14430"
     CDS             complement(2167..>3098)
                     /locus_tag="VHM48_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2756662.1"
                     /translation="NADAALIRARGLTKRFGAFTAVDAVDFSVAKGESFGFLGPNGAG
                     KTSTMRMIGCVSPISDGELEVLGQDPRADGPRIRSRIGVVPQQDTLDTELTVRENLVI
                     YGRYFGLSRAECGRRADELLEFAQLSERANDQVEPLSGGMKRRLTIARALINEPDLLL
                     LDEPTTGLDPQARHLLWDRLYRLKQRGVTLVLTTHYMDEAEQLCDRLVVMDKARIVAE
                     GSPRQLIERYSTKEVTELRFPVGVQETLNGHLEGLAERVELLPDRVLLYTDDGEAAVA
                     AAHQRGLRPETVLVRRSSLEDVFLRLTGRSLIE"
BASE COUNT          529 a         1073 c         1109 g          387 t
ORIGIN      
        1 gacacggctc cgtgccggaa cttcggtcgt ggccgcgtgt cgtcgccggt gagggctgcg
       61 atggtccgga acgacgactc ccaggcgatc gcaccgagcg cgaccagcac ccggacgtcc
      121 gcgagcagcg cgagctcccg caccaggaac ggctggcacg tcgcccgctc cagcggcgtc
      181 ggcttgttgg cgggcggcgc gcagcggacc gcggccgcga tgtaggcgcc ggtcagcgac
      241 aggccatcgt cggcgcgccg accgtccgcc tggttggcga gcccgaccgc gtgcatcgcc
      301 gcgaacagaa agtcgcccga ggcgtcgccg gtgaagaccc gcccggtccg attgccgccg
      361 tgggccgccg gcgccaggcc gacgatgagg atccgcgccc cgggatcccc gaagcccggc
      421 accggtcgcc cccagtaggt ctcgtcgcgg aaccgggcca ctttctcgtg cgcaacctgc
      481 tcgcgccacg ccaccaggcg aggacacgcc cggcaggcga cgatctcggc cgtcagggcg
      541 gcgagctcct cggcgcgatc cacgccgccc accctacttc aggagcagct tgtcgagccg
      601 gcgcgacacg acccacagcc cggcgatccc catcacgccg agatacgcga catggatgaa
      661 catcgccccg tcgaggtggc cgaccgacag cgaccggagc aggtccacgc cctggtagag
      721 cggcgtcagc tggacgacga cccgcagcgc ctccggatag gcgtcgagcg ggtagaacgt
      781 gccggagaac aggaacatcg gcaggacgat gagctggatg aggtcgaagt cctgccagga
      841 ccgcatgaac gatgtcgccg ccatcgcgac cccgccgaag gcgaacccga tcagcagcgc
      901 cgccggcacg gtcaagagca cccacggcga cgcgatcagc ccgaggagcg ccatcaccag
      961 caggaacgcg accgcgtaga tcccgccgcg gatcagcgcc cagacgagct cgccgaccgc
     1021 cacgtcgccg gccgacagcg gcgtcgcgag gatcgcctcg aaggtcttgt tatagcggag
     1081 cttgaagaag aagttgaacg tgccctcggc gatcgcgccg ttcatcgccg cgctcgcgag
     1141 gagggccggc gcaacgaaca gctggtacga gatcggctgg ccgcccggcc ccgtcagggt
     1201 cccgatgagc gcgcccagcc cgaacccgat cgagccgagg tagaagagcg gctcgaagaa
     1261 gcccgacagg atgatgatcc agccgtggcg gtagacgtag atgttgcgct cgacgaggaa
     1321 gcgcgcccgg cggctgccga gcgtgaagag cgaggagagc ggcagcgacg aggccgtcat
     1381 ccgcggacca gccgtgcttc gaccgttcgg aaggcccaga ccgatccgat cgcgagaatc
     1441 gccgccagga ccgcgacatg ggccatggcg agggcggggt tggcgccgat cgtcccgagc
     1501 gccaggccgc ggctcagctc gacgccgtgc cacagcggcg acagccaggc gatcggctgg
     1561 atcgcggccg gcaggttgga gatcgggaag aacgtgccgg agaacaggaa cagcggcgtg
     1621 atcccgaagc ggaacaccgc gttgaacttc tcgggcgtcc gctgggtcgc ggagaacccg
     1681 gcgatcggcg cggcgaaggc catcccggtg aggaccgcag ccgggatgcc caggacgatc
     1741 agcggcgagg cggccgcccc gaacacggtc atgacgaccg tgaagatggc cgagatcgcc
     1801 gtcagccgga tcgcgatcca gacgaggttg ccgacgacga tgtcgcgcgg cgagatcggc
     1861 gtggcgtaca tcgcgtggaa cgtcttctgc caggtcagcc cgcccatgat cgggaacgtc
     1921 gcctcgaagg ccgccgcctg catcgtcgtc gccgccagca ggccgggggc gaggaactgg
     1981 aggtaggaca ggccgcccag cgcggtggtc gccgtcccgg accgatcgac gtacgtcccg
     2041 agcccgaccc ccatcgccag caggaacagc accgggttga ggaaggtcga ggagatcgag
     2101 gcccggacgg tcttccggta ctgcatcgcc cggtgctcga cgacgcgggc catcacggtc
     2161 atcgcgctac tcgatcagcg agcggccggt cagccgcagg aagacgtcct cgaggctcga
     2221 ccggcggacg aggacggtct ccggtcggag gccgcgctgg tgggccgcgg cgacggccgc
     2281 ctcgccgtcg tcggtgtaga gcagcacccg gtccggcagc agctcgaccc gctcggcgag
     2341 gccctcgagg tggccgttga gcgtctcctg gaccccgacc gggaagcgaa gctccgtcac
     2401 ttccttcgtc gaatagcgct cgatcagctg gcgcggtgag ccctcggcga cgatcctggc
     2461 cttgtccatc acgacgagcc ggtcgcacag ctgctcggcc tcgtccatgt agtgggtcgt
     2521 caggacgagc gtcacgccgc gctgcttgag ccggtacagg cgatcccaga ggaggtggcg
     2581 ggcctgcgga tcgaggccgg tcgtcggctc gtcgagaagg agaagatccg gctcgttgat
     2641 cagcgcccgg gcgatcgtca ggcggcgctt catcccgccc gacagcggct cgacctggtc
     2701 gttggctcgc tcgctcagct gggcgaactc gaggagctcg tcggcgcggc gaccgcactc
     2761 cgctcgcgac aggccgaagt accggccgta gatcacgagg ttctcgcgga ccgtcagctc
     2821 ggtgtcgagc gtgtcctgct ggggcacgac gccgatccgg gatcggatcc gcgggccgtc
     2881 cgcccggggg tcctgcccga ggacctcgag ctcgccgtcg ctgatcggtg agacacagcc
     2941 gatcatccgc atcgtcgacg tcttgcccgc cccgttcggc ccgaggaagc cgaacgattc
     3001 gcccttggca accgagaagt cgacggcgtc gacggcggtg aacgcaccga agcgcttggt
     3061 caggccgcgg gcacggatca gggcggcatc ggcattcg
//
LOCUS       DATAIW010000489        16087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_291924, whole genome shotgun sequence.
ACCESSION   DATAIW010000489 DATAIW010000000
VERSION     DATAIW010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291924"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2382)
                     /locus_tag="VHM48_14435"
     CDS             complement(<1..2382)
                     /locus_tag="VHM48_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2756663.1"
                     /translation="MYDASRPESGSSRDLPLRTSPSVAGWIALAIWAMFSLLALLLTV
                     GVVSAFSRYTSDLLPPTDAPKDLSFSQQSVVLDRNGVELARFGGEKREVVEFKDIPPI
                     VVDAQVAVEDKTFWDNAGFDPLAILSAGIDSLRGNSRGASTITQQLVRQRLLEPDLVK
                     DPNRTFERKIKEIVQSIRLTKAYPGVAGKQQIIAAYLNQNYYGNQTYGVKAAAETYFG
                     HPLKDTTPAEAATLAGLVKSPSNYDLVRNAVEDCPADKVDPNDPEKCLVPTTLTVPID
                     TAIYLRRNQILGLLAEGRTVLSGSTYSPAQLEAAKQEPLAVAPQKIPPWKAPHFVWAV
                     LQELADKVCGPATPTCDQLEAGGLTITTTLDTKIQAIAEKWVKAAAVVPNSKNPDSSW
                     KSFKLPGALPQWVTNLSGKDIHNGALVALDYQTGELIAYVGSANYYAAKSTKQFQAKY
                     DVVGSGFRQPGSAFKPFNYLTGINDKKITAGSMLMDTATDFGGKYTPSDADNLERGPV
                     RVRIALQFSLNIPSVKTAQINTPDHIFARAQDFGMVFQGDKTNAGLSIALGVQEVRPV
                     DLVTAYGTLANGGKKVGHTTILSVKNQAGKDVVPAYKPPAGDQVATPQAAFIVTDILA
                     GNTNPSINPFWGKFAVTGPNRERRPATLKTGTNNDAKDLNAYGFIAPPTEGGRAKGEY
                     ALAVGAWNGNSDNTVVSTPGKPIFSIDVTTYVWQGFLQEATKAWAVNDFARPDGVVQA
                     KIDPFTGLKPQPGGKTIDEWFITGTAPQDTIPVGACGQAVLDSPGVHEHDYQNW"
     gene            complement(2467..3474)
                     /locus_tag="VHM48_14440"
     CDS             complement(2467..3474)
                     /locus_tag="VHM48_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX2756664.1"
                     /translation="MTTPAVLAVDGGNSKADVALVAADGTLLGAVRGPTISHQAGPLS
                     RGTRRLAELVAAAAGSGESRASIAVHCVAGADYPSDVRLLERAFASVAAAGETLVLND
                     TFAALRAGATRPWGIVLICGQGINGAAVGVDDRRVRFAGVGDIAGDWGGAGSVGMAGL
                     GAAVRARDGRGPRTALETTIPGAVGLVRPEAVTHAYYSGRLDERRIGELAPTVFATAA
                     AGDPVARSIIDRLADELATMAIALARRAHLVRREPEVVLAGSVFRTGDAGFHERLLTA
                     IGAAIPGARVGRLTAPPVVGAALIGLDRLGGGTVDQAVEARVRAAFDEWNATPIVVTP
                     S"
     gene            complement(3471..4157)
                     /locus_tag="VHM48_14445"
     CDS             complement(3471..4157)
                     /locus_tag="VHM48_14445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2756665.1"
                     /translation="MGTSIDAGGRFRVALTFDAEHPDRPHRPGVTAGIIEVLAERHVP
                     STWFLQGRWVESAPELARQVVADGHLVGSHSFYHTRLPLLTDDGIATDLPDAERVIRE
                     VTGVDPRPWFRCPFSAGADDPRVLGILDGLGYRDIGADVILEDWEPSRTGANLTADAL
                     RDTPAFGDGAVVLFHAWPPGTLDALPLIVDGLRALGAAFVRIDELERFARPSGAGSAP
                     APRSREQPAP"
     gene            complement(4154..5248)
                     /locus_tag="VHM48_14450"
     CDS             complement(4154..5248)
                     /locus_tag="VHM48_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2756666.1"
                     /translation="MAVANPLMRDVVAPAAAPRGRRRFRPREAGKSFLFTFIVVVVLA
                     AFLSPLIRSVFVSLQTPDQVGQLDAPPYPAVHATFDYQGKTYDVYNVPIDGATKSLAL
                     VIKGRESSKFVDPTNPSGGLIVWQGSWRNLDRSWSFAPEWSNYSTVWDQIDFPRLLFN
                     TVAIALIGMIGTILSCTLVAYGFARFRFPGRSLLFTILIATIFLPYAVTIIPTYTVFR
                     ELGWVGTWLPLLVPTFFANAYDVFLLRQYLLTIPREMDEAAAIDGAGPFRTLISVIVP
                     QAWPAIIAVSIFHIVYAWNDFFGPLIYLSTKPELQTLALGLAHFNTLHYRNQAFIQVG
                     TLLTMVIPILVFLVFQRFFTRGLVISGVEK"
     gene            complement(5250..6236)
                     /locus_tag="VHM48_14455"
     CDS             complement(5250..6236)
                     /locus_tag="VHM48_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2756667.1"
                     /translation="MAAVPIQPVIPRRTGRRGPLRLRRPSLGKSALSRKEARWGLLFL
                     SPWLIGFFAFTFLPMLASLAFSLTNLSLSQDQPLRFVGLENFQRMLADSQTWESLAVT
                     FRFAAFNLPIAMVIPFVVALVLNSRYLRGVAIFRTLFFMPYVIPFVSGLLAWQGMLNL
                     ETGWVNEFLRMLGVQNPPNWLQDTTLIYPSLAFIGLWGIGAGVIVNLAGLKGIPSELY
                     DAARIDGAGWWATLRNVTLPMMSPVIFYSLILGIVDVLQYFLVPLVLYNGTGEPGGST
                     LFFNLVIYKQAFNFYNFSYGATLAWMLFAITLAITLVVFRTSRRWVYYAGER"
     gene            complement(6313..7782)
                     /locus_tag="VHM48_14460"
     CDS             complement(6313..7782)
                     /locus_tag="VHM48_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756668.1"
                     /translation="MPARPFRPVVLIAAIALLASACGGSTATNPPASGGAPSASVAAS
                     VAPPSTGPATVPPGGPVEIRWYCCLGGGEAPEQKSVEDKVVAAFNASHPNIHLTFEVV
                     TYDQANGQLATEIQSGNGPDIVGPVGIGGANAFHGQWLDLAPLITKSNYDLSGFPSNV
                     VDIYKLDEGQVGIPFAIYPSALFYKKGLFEEANLSEPPHKYGDKYTMPDGSQVDWNYD
                     TALQVAKLLTVDKNNKDAAQTGFDPKKIAQWGLELQRDDLRGLGAYFGSGSLAGADGK
                     TVQIPDAWKAAWRYYYNAIWTDHVSLTGPQFESKDINPKDYPFFSGHTAMSENFLWST
                     YGVADAGDDWDLAAIPSYNGQVTAAFNADTFRILKGSKHPDEAFQVLTYLLGDGAPDL
                     LKAYGGFPARTADQQAGIDALQSQFKNTVDWQVAIDGIVKADNPNFESFMPAYNESLT
                     LVGSGGKYLTNWGNKPGLDMDKEIQALQDELQKIWDKAG"
     gene            complement(7810..8658)
                     /locus_tag="VHM48_14465"
     CDS             complement(7810..8658)
                     /locus_tag="VHM48_14465"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2756669.1"
                     /translation="MARFRFELGPVPLHHQVYLDLRAALDNGEWRPGDLLPPERQLAE
                     RYGCSLITVRRALSELTREGRIQRTRGRGTTVLPPRIERDFGGTLSFTEEMQDRGLDP
                     ETRLIGSRPESAGEVVAGALGIEPGSPTLYVERLRVAGGEPLLLEQVHLPAERFPGLL
                     ASDLEHGSLYDLLTIRYGTRIVRAREALEPVLLRAREARLLAQKPRTPALLIEGTAFG
                     ADGAPVEFARSYVRGDRTRYYVERVVVRASWQADPDTTVEPGRDARLATAGSRGRATP
                     TKVPTG"
     gene            8824..10086
                     /locus_tag="VHM48_14470"
     CDS             8824..10086
                     /locus_tag="VHM48_14470"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756670.1"
                     /translation="MAVVGGGSTYTPELVDGFTKRADRLPVDEIALMDIDPERLAIVG
                     GLAERMLARQGWTGRVVRTTDRDAAIDGADFVLVQLRVGGQQARFGDETLPPRFGLIG
                     QETVGAGGFAKALRTVPIVLELADAVERRSAPGAWIVDFTNPVGIVSQALADAGRRFV
                     GLCNVAIGFQRRFAARFDVDPERVELEQVGLNHLTWIRAVRIDGVDRLPEILAEDAAD
                     LAAEIKVPEGRLTSRRVIPSYYLRYYDDEALVVTEAKAHPTRAEEVMAIERELLELYR
                     DPLLDTKPELLEHRGGAFYSEAAAQLIASLTDGRGDQQIVDVRNDGTLPGLADSDVVE
                     VPARIDRDGAHPIPQAPLSAPQADLVGRVKVYERLTVRAAISGDRAIARAALAANPLA
                     GGPDGAEELLDAILAANRHWLPRFFPDG"
     gene            10090..11181
                     /locus_tag="VHM48_14475"
     CDS             10090..11181
                     /locus_tag="VHM48_14475"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2756671.1"
                     /translation="MTGRTVGRTAMRGEIEAVPDVVGRFLAEAGPEVEAAAAAIRAAD
                     PAWVTLVARGTSDHAAIHLRYLIETELGIPAGMAAPSTVTIYGADLRWRGGLVIAVSQ
                     SGRSPDLVAVVAAARAGGAVTVAIVNDAESPLAAAADHVIDCRAGEERSVAATKSYVA
                     QLAAGASLVAALARSGGVRDALPRLPRILADSLAVATGVVHDESAIVAEFGASERSIV
                     IGRGFEYPTALETALKLKETCRLFAEGYSSADFSHGPVVLTGPEVPILAIRPDGRMGP
                     LVDEGIVAALASGSVPWVVGGAAVAAAAPAGLDPARVISLPLPADLPEGLASLATILP
                     GQLLAEAVARRRGYDPDAPPGLHKVTLTR"
     gene            11263..11706
                     /locus_tag="VHM48_14480"
     CDS             11263..11706
                     /locus_tag="VHM48_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX2756672.1"
                     /translation="MDAANPIIRALDAAGYRVTSPRRSLADLVIDLDGHFTASELAAA
                     GRQRGLRIGRATLFRALELMIELGVVERLDMPSGEHTYVACAPAHHHHVVCSRCGRTA
                     EVADAGLAGAVRDIERQSGFRIDAHRLELFGLCRHCLARASDKRP"
     gene            11706..12632
                     /locus_tag="VHM48_14485"
     CDS             11706..12632
                     /locus_tag="VHM48_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX2756673.1"
                     /translation="MRLGRIGWTLVIAVIAASLMLAGCGPDRASVGPSGPAPDADALR
                     VVTTTTVLADIVAAVGGRRTSVHSIIPPGVGPEDYEAKPDDARSLADAQLIVSNGVGL
                     DDFLNRLLASGGGGTTPRLVLGEGLPTIDVDGEPNPHFWLDPTIVKTGYLPKIVESLS
                     AAAPADRATFESNAAAYGTRLDALDAELQAEVATIPAANRKLVTFHDAFPYFARHFGF
                     ELVGVVVANVGQEPNAAELAKLVDKVKVTGARAVFSEAQFNPKLAQTLADEAGVSKVV
                     TTLYNDALGPAPADTYPGMMRWNVQQIVEALR"
     gene            12665..13510
                     /locus_tag="VHM48_14490"
     CDS             12665..13510
                     /locus_tag="VHM48_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX2756674.1"
                     /translation="MPAGRAVVAPGEPTVVLDHVTAGYGRRVALADVDLTVRAGSLLA
                     VIGPNGAGKSTLLKLIAGLLKPDTGTIEVLGAAPGVEARSVAYVPQAEAVDWDFPVTV
                     GEVAMMGRYARLGFGRRPGHHDHHVVGAALETVGMHDQFDRQIGALSGGQRRRVFLAR
                     AIAAEPELYLLDEPVTGVDAKTQEELMDVLEAEARAGKTVIASTHDLICAAQRFHQAA
                     FVNGRIVATGPAEMVLDRKLLSDTYGGHVLVLPGDDGGRLILDDAHHHDQAPGGESHY
                     HEGSH"
     gene            13510..14382
                     /locus_tag="VHM48_14495"
     CDS             13510..14382
                     /locus_tag="VHM48_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX2756675.1"
                     /translation="MDPIGFLADPLAYGFMQRGLAAAVLVGIVCAVMGTFVVLKGLAF
                     IGDAVSHAAFPGLVIAFMLGLPLYLGGAVASVGTALAIGFVSRRGRLRFDTTVGVLFA
                     GTFALGILLFSTIKNYVADLLGYLLGNVLGIGIGDLIQVAVLGAVVLVIVIAIRKELL
                     FATFDPLGAAASGLPVGALEYLLLALLGVTIVVSIQAVGIVMVVAMLVTPAATAQLLV
                     VRFERLMLLAALFATVAAVSGLYLSFYANLASGASIVLIETLFFLVALLFGPRSGLLS
                     GRLARRRSEGLEPA"
     gene            complement(14466..15602)
                     /locus_tag="VHM48_14500"
     CDS             complement(14466..15602)
                     /locus_tag="VHM48_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756676.1"
                     /translation="MTTSHPAPTHVPSASPREAIDPFAWTKIGEMPYPGGELLKGWFS
                     AGYLALGWVEQNGDLAPVASFSRDGRTWRSADLTTPVTPCPGWTARPDGSVADGWAAG
                     GGAVFVGMETVLHGETCDQTRPVAWISSDGLDWTRTHAFEPAGLPITYPTAVWTTPSG
                     WETALKETDDRTWLWQSADAATWERRGMISANPDLGLIVVANDGTRVVVASDDAGRSR
                     LLSTSTDGRTWRAIAGPPVVSTGPGDDWQVIRVLAPDGPAPFWTVVASGEEGPDAHAT
                     IWTSADLETWQSGRFPMPSISALGVTSDGILALGTDPCSITGSACPPTRPPRWFRSLD
                     GLTWLPLASTRGPDTLMEGPAGTIGIGAPSGNGRPAPVYRLERR"
BASE COUNT         2326 a         5426 c         5859 g         2476 t
ORIGIN      
        1 ccagttctgg tagtcgtgct cgtggacgcc gggcgagtcg aggacggcct ggccgcaggc
       61 gccgaccggg atcgtgtcct ggggcgccgt gccggtgatg aaccactcgt cgatcgtctt
      121 gccgccgggc tgcggcttga gccccgtgaa cgggtcgatc tttgcctgga cgacgccgtc
      181 cggtcgagcg aagtcattga ccgcccaggc cttcgtggcc tcctggagga agccctgcca
      241 gacgtaggtc gtcacgtcga tcgagaagat cggcttgcca ggcgtcgaga cgaccgtgtt
      301 atcgctgttg ccgttccagg cgccgactgc gagggcgtac tcgcccttcg cccgcccgcc
      361 ctcggtcggc ggtgcgatga agccgtaggc gttgaggtcc ttggcgtcgt tgttcgtgcc
      421 ggtcttcagg gtcgccggcc ggcgctccct gttgggaccg gtcacggcga acttgcccca
      481 gaacggattg atgctcggat tcgtgttgcc ggccaggatg tcggtgacga tgaaggccgc
      541 ctgaggggtc gcgacctggt cgccggccgg cggcttgtag gccgggacga catctttccc
      601 ggcctggttc ttgacgctca ggatcgtcgt gtggccgacc ttctttccgc cgttggcgag
      661 ggtcccatag gcggtgacga ggtcgaccgg gcggacctcc tggacgccga gcgcgatcga
      721 cagcccggcg ttggtcttgt cgccctggaa caccatcccg aagtcctggg cgcgggcgaa
      781 gatgtgatcc ggcgtgttga tctgggcggt cttgaccgac gggatgttga gcgagaactg
      841 gagcgcgatc cggacgcgga ccggccctcg ctcgaggttg tcggcatccg acggggtgta
      901 cttcccgccg aagtcggtcg cagtatccat gagcatcgag ccggcggtga tcttcttgtc
      961 gttgatcccg gtcaggtagt tgaacggctt gaacgccgac cccggctggc ggaagccgct
     1021 gccgacgacg tcgtacttgg cctggaactg cttggtgctc ttggccgcgt agtagttggc
     1081 gctgccgacg taggcgatca gctcaccggt ctggtagtcg agggcgacga gcgctccgtt
     1141 gtggatgtcc ttgccgctca ggttcgtcac ccactgaggc agcgcgccgg gcaacttgaa
     1201 cgatttccag ctcgagtcgg gattcttcga attgggcacg acggcggccg ccttgaccca
     1261 cttttccgcg atcgcctgga tcttcgtgtc gagggtggtc gtgatcgtga gaccgccggc
     1321 ctcgagctgg tcgcaggtcg gggtggccgg accgcagacc ttgtcggcga gctcctggag
     1381 gaccgcccag acgaagtgcg gcgccttcca cggcggaatc ttctgcggcg cgaccgcaag
     1441 tggctcctgc ttcgcggcct cgagctgggc ggggctgtag gtggagcccg acaggactgt
     1501 ccggccctcc gcgagcaggc ccaggatctg gttgcgccgc aggtagatcg cggtgtctat
     1561 cgggacggtc agggtggtcg ggacgaggca cttctcggga tcgttcgggt cgaccttgtc
     1621 cgccgggcag tcctcgacgg cattgcggac caggtcgtag ttcgacggcg acttgacgag
     1681 cccggcgagc gtcgccgcct cggccggggt cgtgtccttg agcgggtgcc cgaagtacgt
     1741 ctcggctgcg gccttcaccc cgtaggtctg gttgccgtag tagttctggt tgaggtaggc
     1801 cgcgatgatc tgctgcttgc ccgcgacgcc cgggtaggcc ttcgtcagcc ggatcgactg
     1861 gacgatctcc ttgatcttcc gttcgaacgt tcggttgggg tccttcacga ggtcgggctc
     1921 gagcaggcgc tggcggacga gctgctgcgt gatcgtcgag gcgccgcggc tgttgccgcg
     1981 gaggctgtcg atcccggccg acaggatcgc gagcgggtcg aaaccggcgt tgtcccagaa
     2041 ggtcttgtcc tcgaccgcga cctgggcgtc gacgacgatc ggcgggatgt ccttgaactc
     2101 gacgacctcg cgcttctccc cgccgaagcg ggccagctcg acgccgttgc ggtcgaggac
     2161 gaccgactgc tggctgaagc tgaggtcctt gggcgcgtcg gtcgggggca gcagatcgga
     2221 ggtgtagcgc gagaaggcgc tgacgacgcc gaccgtcagg agcagggcga gcagcgagaa
     2281 catcgcccag atcgcgaggg cgatccagcc ggcgaccgag ggggacgttc gcagcggcag
     2341 gtcgcggctc gagccgctct ccgggcgaga ggcgtcgtac acgtggcgac tccgggaagg
     2401 aatggtgtct ggcgaagcat gccatccggc gggtgatgcg gcgcgcgagc gcccggccga
     2461 acggtgctac gacggcgtga caacgatggg tgtggcgttc cactcgtcga acgcggcacg
     2521 gacccgggcc tcgaccgcct ggtccacggt gccgccgccg aggcggtcga ggccgatcag
     2581 ggcggccccg acgacgggtg gcgccgtgag gcgtccgacg cgggcgcccg ggatcgccgc
     2641 gccgatcgcg gtcaggaggc gttcgtggaa cccggcatca ccggtccgga acacgctgcc
     2701 ggccaggacg acctcgggct cgcgcctgac gagatgcgcc cgccgggcca gggcgatcgc
     2761 catcgtcgcg agctcgtcgg cgagccggtc gatgatcgag cgggccaccg gatcgcccgc
     2821 ggcggcggtc gcgaagacgg tcggggcgag ctcgccgatc cgccgctcgt ccaaccgccc
     2881 cgagtagtag gcgtgggtga ccgcctccgg tcggaccagg ccgacggcgc ccgggatcgt
     2941 cgtctcgagc gccgtccgcg gcccgcggcc gtcccgcgcc cggaccgccg caccgagtcc
     3001 ggccatcccg accgagccgg ccccgcccca gtcgccggca atgtcgccga cgcccgcgaa
     3061 acggacccgt cgatcgtcga cgccgacggc cgcgccgttg atcccctggc cgcagatcag
     3121 gacgatgccc cacggccggg tggcgccggc ccgaagggcg gcgaaggtgt cgttgaggac
     3181 gagcgtctcg ccggccgctg cgaccgacgc gaaggcccgt tcgaggaggc ggacatcgct
     3241 cggatagtcg gcgccggcca cgcagtgcac cgcgatcgag gcgcgcgact cgccgctgcc
     3301 tgcggccgcg gcgaccagct ccgcgagccg gcgcgtccct cgggagaggg gaccggcctg
     3361 gtgcgagatc gttgggccgc gcaccgcgcc gagcagcgtg ccgtcggccg cgacgagcgc
     3421 cacgtcggcc ttggagttgc cgccgtcgac ggccaggacg gccggcgtcg tcacggggcg
     3481 ggctgttcgc gcgaacgtgg ggccggcgcc gatcccgcgc cggacggacg ggcgaaccgc
     3541 tcgagctcgt cgatcctgac gaaggctgcg ccgagcgcgc ggaggccgtc gacgatcagc
     3601 ggcagggcgt cgagcgtgcc gggtggccag gcgtggaaga ggaccactgc tccgtcgccg
     3661 aacgccggcg tgtcgcgcag ggcgtcggcg gtcaggttcg ccccggtccg cgacggctcc
     3721 cagtcctcga ggatgacgtc ggcgccgatg tcgcggtagc cgaggccgtc gaggatcccg
     3781 aggacccgcg gatcgtcggc cccggccgag aacgggcagc ggaaccaggg ccgcggatcg
     3841 acaccggtca cctcgcggat cacgcgctcg gcgtccggca ggtcggtggc gatgccgtcg
     3901 tcggtcagga gcggcaggcg ggtgtgatag aagctgtggc tgccgacgag gtgcccgtcg
     3961 gcgacgacct gccgcgcgag ctcgggcgcg gactcgaccc agcggccctg gaggaaccag
     4021 gtgctcggga cgtgacgctc ggcgagaacc tcgatgatcc cggcggtcac gcccggccga
     4081 tggggccggt ccgggtgctc ggcatcgaag gtcagggcga cccggaaccg cccgcccgcg
     4141 tcgattgagg tgcctatttc tcgactccgc tgatcacgag gccgcgggtg aagaagcgct
     4201 ggaacacgag gaagaccaag atcgggatca ccatcgtgag cagcgtaccg acctggatga
     4261 acgcctggtt gcggtaatgc agcgtgttga agtgggccag gccgagggcc agggtctgga
     4321 gctccggctt cgtcgagagg tagatgagcg ggccgaagaa gtcgttccag gcgtacacga
     4381 tatggaagat cgagacggcg atgatcgcgg gccaggcctg cggcacgatc accgagatca
     4441 gggtccggaa cgggccggcc ccgtcgatcg cggcggcttc gtccatctcc cgcgggatcg
     4501 tcaggaggta ttggcgcagg aggaacacgt cgtaggcgtt ggcgaagaac gtcggaacga
     4561 gcagcggcag ccacgtcccg acccagccca gctcgcggaa caccgtgtag gtcgggatga
     4621 tcgtgaccgc gtagggcagg aagatcgtcg cgatcaggat cgtgaacagg aggctccgac
     4681 cggggaaccg gaaccgggcg aatccgtagg caacgagcgt gcacgacagg atcgttccga
     4741 tcatcccgat cagggcgatc gcgaccgtgt tgaagaggag tcgcgggaag tcgatctggt
     4801 cccagaccgt cgagtagttc gaccactccg gcgcgaatga ccatgaccga tcgaggttgc
     4861 gccacgatcc ctgccagacg atcaggccgc ccgagggatt ggtcgggtcg acgaacttgc
     4921 tcgattcacg acccttgatg acgagcgcaa gcgacttcgt cgcgccgtcg atcggcacgt
     4981 tgtacacgtc gtaggtcttg ccctggtagt cgaacgtcgc atggacggcc gggtacggcg
     5041 gcgcgtcgag ctggccgacc tggtccgggg tctggagcga gacgaacacc gaccggatga
     5101 gcggtgagag gaaggcggcg aggacgacga cgacgatgaa cgtgaacagg aacgacttgc
     5161 ccgcctccct gggcctgaat cggcgccgac cgcggggcgc cgcagccggc gccacgacgt
     5221 cccgcatcag cgggttggcg accgccacgt cagcgctctc cggcgtagta gacccagcgc
     5281 cgcgaggtgc ggaacaccac cagcgtgatc gccagcgtga tcgcgaacag catccaggcg
     5341 agggtcgcgc cgtaggagaa gttgtagaag ttgaaggcct gcttgtagat cacgaggttg
     5401 aagaacaggg tcgagccgcc cggctcgccg gtcccgttgt agagcacgag cggcacgagg
     5461 aagtactgga ggacgtcgac gatgccgagg atgagcgagt agaagatgac cggcgacatc
     5521 atcggcaggg tcacgttgcg gagggtcgcc caccagcccg ccccgtcgat ccgggcggcg
     5581 tcatacagct cgctcggaat gcccttgagg ccggccaggt tgacgatgac tccggccccg
     5641 atcccccaca gcccgatgaa cgccaggctc gggtagatca gggtggtgtc ctggagccag
     5701 ttgggcgggt tctggacgcc gagcatccgg aggaactcgt tgacccagcc ggtctcgaga
     5761 ttcagcatcc cctgccaggc cagcaggccc gacacgaacg ggatgacgta gggcatgaag
     5821 aacagcgtcc ggaagatcgc gacgccgcgc aggtagcggc tgttgagcac gagcgcgacg
     5881 acgaacggga tcaccatcgc gatcggcagg ttgaatgccg cgaaccggaa ggtgaccgcg
     5941 agcgactccc acgtctggga gtcggccagc atccgctgga agttctcgag gccgacgaaa
     6001 cgcaggggct gatcctgcga gaggctcagg ttcgtcaggc tgaaggcgag cgaggcgagc
     6061 atcggcagga aggtgaacgc gaagaagccg atcagccacg gcgacaggaa gagcaggccc
     6121 cagcgggcct ccttgcggct cagggccgac ttgccgagcg agggtcggcg caggcgcaac
     6181 gggccgcgtc gcccggtccg tcgcgggatc accggctgga tgggaacggc ggccacgcgt
     6241 cctcctgctg acgccctccg cccgccggga ccggcaggcg gagaagtggt tgacgatcga
     6301 gcgggcggtt gctcagccgg ccttgtccca gatcttctgg agctcgtcct ggagtgcctg
     6361 gatctccttg tccatgtcga gacccggctt gttgccccag ttcgtgaggt acttgccgcc
     6421 cgacccgacg agggtgaggc tctcgttgta ggcgggcatg aacgactcga agttggggtt
     6481 gtcggccttt acgatcccgt cgatcgcgac ctgccagtcg acggtgttct tgaattgtga
     6541 ctggagggcg tcgatgccgg cctgctggtc ggcggtccgg gcggggaacc cgccataggc
     6601 cttgaggagg tccggcgcgc catctccgag gagataggtc aggacctgga acgcctcgtc
     6661 ggggtgcttc gatcccttga ggatccggaa cgtatcggcg ttgaaggccg ccgtcacctg
     6721 gccgttgtac gacggaatgg cggcgaggtc ccagtcgtcg ccggcgtcgg cgacgccgta
     6781 cgtcgaccag aggaagttct cgctcatcgc ggtgtgaccg ctgaagaacg gatagtcctt
     6841 ggggttgatg tccttgctct cgaactgggg gccggtgagg ctgacgtggt ccgtccagat
     6901 cgcgttgtag tagtacctcc acgccgcctt ccaggcgtcc gggatctgga cggtcttgcc
     6961 gtccgcgccg gcgagcgatc cggagccgaa gtaggcaccc aacccgcgga ggtcgtcgcg
     7021 ctggagctcg agcccccact gggcgatctt cttcggatcg aagccggtct gggccgcatc
     7081 cttgttgttc ttgtcgacgg tcagcagctt ggcgacctgg agcgcggtgt cgtagttcca
     7141 gtcgacctgg ctgccgtcag gcatcgtgta cttgtcgccg tacttgtggg gtggctcgct
     7201 gaggttggcc tcctcgaaga ggcccttctt gtagaacagg gccgacgggt agatcgcgaa
     7261 cgggatgccg acctggccct cgtcgagctt gtagatgtcg acgacgttcg aggggaagcc
     7321 ggacaggtcg tagttgctct tcgtgatcag gggcgcaagg tcgagccact ggccgtggaa
     7381 cgcattggcg ccgccgatcc ccacgggacc gacgatgtcc ggcccgttgc cggactggat
     7441 ctcggtcgcg agctggccgt tcgcctgatc gtaggtcacg acctcgaagg tcaggtggat
     7501 gttcggatgc gaggcgttga aggcggcgac gaccttgtcc tcgaccgact tctgctcggg
     7561 cgcctcgcca ccgccgaggc agcagtacca gcggatctcg acggggccgc cgggcgggac
     7621 ggtcgccggg ccggtcgatg gcggggccac cgatgcggcg acggacgccg acggcgcgcc
     7681 gccggacgcc ggtggattgg tcgccgtcga cccgccgcag gcgctcgcca gcagggcgat
     7741 cgccgcgatc aggacgacgg gacggaacgg tcgcgcgggc atggctcctc ctccttgcga
     7801 tgtggccgat cagccggtgg gtaccttcgt cggagtggcc cgaccacggc tcccagccgt
     7861 ggccagccgc gcgtcgcggc cgggttcgac ggtcgtgtcc ggatcggcct gccacgaggc
     7921 ccggacgacg acccgttcga cgtagtaacg cgtccggtcg ccgcggacat agctccgggc
     7981 gaactcgacc ggtgcgccgt ccgcgccgaa cgcggtgccc tcgatcagga gggccggggt
     8041 gcgcggcttc tgggcgagga ggcgcgcctc gcgggcccgc aggaggacgg gttcgagcgc
     8101 ttcccgggcg cgaacgatcc gggttccgta gcggatggtc agcaggtcgt agagcgagcc
     8161 gtgctcgagg tccgacgcga ggaggcccgg gaagcgctcg gccggcaggt ggacctgctc
     8221 gaggaggagc ggctcgccgc cggcgacccg cagccgctcg acgtacagcg tcggcgagcc
     8281 cggctcgatc ccgagcgccc ccgcgacgac ctcgccggcc gattccggac gggagccgat
     8341 gagccgcgtc tccgggtcga gaccgcgatc ctgcatctcc tcggtgaagc tcaacgtgcc
     8401 gccgaagtcg cgctcgatcc gcggtgggag gacggtcgtg ccgcgacccc gggtccgctg
     8461 gatgcgccct tcgcgggtca gctcggagag cgcccgccgg accgtgatca ggctgcagcc
     8521 gtagcgctcg gcgagctggc gctccggcgg gaggagatcg ccgggacgcc actcgccgtt
     8581 gtcgagcgct gcgcgcaggt ccaggtagac ctggtggtgg agcgggacgg gaccgagctc
     8641 gaatcggaaa cgggccatcg agtggagacc tcgcgggacg atcctggaac gggcttcgcg
     8701 gatgttcgtc gggctgcttg cgcgacggat gaatgctgat aacgttataa gcaatctgtc
     8761 aagccgctgc gcatcccggc cggcggcgga cggacggacg agcgaggagc catgggtctg
     8821 aagatggccg tcgtcggtgg cggcagcacc tacacgcccg agctggtcga cggcttcacc
     8881 aaacgcgccg accgcctccc cgtcgatgag atcgcgctca tggacatcga cccggaacgt
     8941 ctcgcgatcg tcggcggcct ggccgagcga atgctggcac gccagggctg gaccgggcgc
     9001 gtcgtccgga cgaccgaccg cgacgcggcg atcgacggcg cggatttcgt cctcgtccag
     9061 cttcgtgtcg gcggccagca ggcccggttc ggggacgaga cgctgccgcc ccggttcggc
     9121 ctgatcggcc aggagaccgt cggcgccggc ggcttcgcga aggcccttcg gaccgtcccg
     9181 atcgtcctcg agctggccga cgcggtcgag cgacggtccg cgcccggcgc ctggatcgtc
     9241 gacttcacca acccggtcgg gatcgtcagc caggccctcg ccgacgccgg ccggcggttc
     9301 gtcggcttgt gcaacgtcgc gatcgggttc cagcgccggt tcgccgcccg gttcgacgtc
     9361 gatccggagc gggtcgagct cgagcaggtc gggctcaacc acctcacctg gatccgggcc
     9421 gtccggatcg acggggtcga tcgcctgccg gagatcctcg ccgaggacgc cgcggatctc
     9481 gccgcggaga tcaaggtccc ggaggggcgc ctgacgagcc ggcgcgtgat cccgtcctac
     9541 tacctccgct actacgacga cgaggccctc gtcgtcaccg aggcgaaggc ccatccgacc
     9601 cgggccgagg aggtcatggc gatcgagcgc gagctcctcg agctctatcg cgatccgctg
     9661 ctcgacacga agccggagct cctcgagcac cgcggcggcg cgttctacag cgaggcggca
     9721 gcccagctga tcgcgtcgct gaccgacggt cgcggcgacc agcagatcgt cgatgtccgc
     9781 aatgacggga ccctgccggg cctcgcggac agcgacgtgg tcgaggtgcc ggcccggatc
     9841 gatcgggatg gcgcccatcc gatcccgcag gcgccgttgt cggcgccgca ggccgatctc
     9901 gtcggccggg tcaaggtgta cgagcggctg acggtccggg cggcgatcag cggcgaccgg
     9961 gcgatcgccc gtgctgcgct cgccgccaac ccgctggccg gcggtccgga tggcgctgag
    10021 gagttgctcg acgcaatcct ggcggcgaac cggcactggc tgccgcgctt cttccccgat
    10081 ggctagtcga tgaccggccg gacggtcggc cggaccgcga tgcgcggcga gatcgaggcg
    10141 gtcccggacg tggtcgggcg atttctcgcc gaggcggggc cggaggtcga ggcggcggcc
    10201 gccgcgatcc gggcggccga tccggcctgg gtcaccctcg tcgcccgcgg gacctctgac
    10261 catgccgcga tccatctccg ctatctgatc gagactgagc tcgggatccc ggccgggatg
    10321 gccgccccat cgacggtcac gatctacggt gccgaccttc gctggcgggg cggcctggtc
    10381 atcgccgtct cgcaatccgg ccggtcgccg gacctcgtcg ccgtcgtcgc tgccgcccgg
    10441 gccggcggtg ccgtgacggt ggccatcgtc aatgacgccg aatccccgct cgcggcggcg
    10501 gccgaccacg tgattgactg ccgggcaggg gaggagcgat cggtcgcggc gacgaagtcc
    10561 tacgtggccc agcttgcggc gggggcgtcg ctggtcgcag cgcttgctcg atcggggggc
    10621 gtccgcgacg ccctgcctcg cttgccgcgg atcctcgcgg actccctggc ggtcgcgacg
    10681 ggcgtggtcc acgacgagtc ggcgatcgtg gccgaattcg gcgcgtccga gcggtcgatc
    10741 gtcatcggcc ggggcttcga gtatccgacg gccctcgaga cggccctcaa gctcaaggag
    10801 acgtgccggc tcttcgccga gggctactca tcggccgact tcagtcacgg gcccgtggtc
    10861 ctgaccgggc ccgaggtgcc gatcctcgcg atccgacccg acggccgcat gggcccgctg
    10921 gtcgacgagg ggatcgttgc cgccctggcc agcgggagcg tcccctgggt cgtcggcggc
    10981 gcggcggtcg ccgccgctgc gccggccggc ctcgatccgg cccgggtgat ctccctgccc
    11041 ctgcccgccg accttcccga ggggctggcg tccctcgcga cgatcctgcc cggccagctc
    11101 ctcgccgagg cggtcgcgcg ccgccgtggc tacgaccccg acgccccgcc cgggcttcac
    11161 aaggtgacgc tcacgcgctg atcgccgccc gccgccgttc tggacggtga tgttgagact
    11221 gagtctcgac aagagaaggg tggccgccgt atcctcacgg cgatggacgc agccaacccg
    11281 atcatccggg cgctcgatgc cgccggctat cgggtcacct ctccgcgacg gtccctcgcc
    11341 gacctcgtca tcgatctcga cggccacttc acggcatccg aactggccgc cgcgggtcgc
    11401 cagcggggtc tgcggatcgg ccgggcgacg ctgttccgtg ccctcgagct gatgatcgag
    11461 ctcggggtcg tggaacggct cgacatgccg agcggcgagc acacgtacgt cgcgtgcgcg
    11521 ccggcccacc accaccacgt cgtctgctcg cgttgcggca ggacggccga ggtcgccgac
    11581 gccggactgg ccggagccgt ccgcgacatc gagcggcaga gcggattccg gatcgatgcc
    11641 caccgcctgg agctgttcgg gctatgccgc cactgcctcg ccagggcatc ggacaaacgg
    11701 ccgtgatgag actcggtcgc atcggttgga cgctcgtcat cgccgtgatc gcggcgtcgc
    11761 tcatgctcgc cgggtgcggc cccgaccggg catcggtcgg cccctcgggc ccggctcccg
    11821 acgcggatgc gctgcgtgtc gtgaccacga caaccgtcct cgccgacatc gtcgcggcgg
    11881 tgggaggccg gcgaacgagc gtccactcga tcatcccacc cggtgtcggc ccggaggact
    11941 acgaggccaa gcccgacgac gcccggagcc tcgccgacgc ccaactcatc gtctcgaacg
    12001 gcgtcggtct cgatgatttc ctcaaccggc tcctcgcctc cggcggcggc gggacgacgc
    12061 cgcggctggt cctcggcgag ggtctcccga cgatcgacgt cgacggcgag ccgaacccgc
    12121 acttctggct cgacccgacg atcgtcaaga cgggctatct gccgaagatc gtcgaatcgc
    12181 tgtccgccgc ggcgccggcg gatcgcgcca cgttcgagtc gaacgccgcg gcctacggga
    12241 cgcggctcga cgcgctcgat gccgagctgc aggcggaggt cgcgacgatt cccgccgcga
    12301 accgcaagct cgtcaccttc cacgacgcct ttccctactt cgctcgtcat ttcgggttcg
    12361 agctcgtcgg ggtcgtggtc gccaacgtcg gccaggagcc caatgccgcc gagcttgcca
    12421 agctcgtcga caaggtcaag gtgaccgggg ctcgggcggt cttctcggag gcccagttca
    12481 acccgaagct cgcccagacc cttgccgacg aggcgggggt gagcaaggtc gtcacgacgc
    12541 tctacaacga cgccctcggg cccgccccgg cggacaccta tccgggcatg atgcgctgga
    12601 acgtgcaaca gatcgtggag gccctccgct gagcaccatg cccgccccga accccatcgg
    12661 tcccgtgccc gccggccgcg ccgttgtcgc accgggcgaa ccgaccgtcg tcctcgacca
    12721 cgtgaccgcc ggttacgggc ggcgggtcgc cctcgccgac gtcgacctga ccgtccgggc
    12781 gggttcgctg ctcgccgtca tcggaccgaa tggcgccggc aagagcacgc tcctcaagct
    12841 gatcgcgggg ctgctgaagc cggacaccgg gacgatcgag gtcctcggtg ccgcgcccgg
    12901 ggtcgaggca cgctcggtgg cgtacgtgcc gcaggccgag gccgtcgact gggatttccc
    12961 ggtgaccgtc ggcgaggtgg ccatgatggg ccgctatgcc cgtctcgggt tcgggcggcg
    13021 gcccgggcat cacgaccatc acgtcgtcgg cgcggcgctg gagacggtcg ggatgcacga
    13081 ccagttcgat cgccagatcg gcgcgctgtc cggcggtcag cggcggcggg tgttcctggc
    13141 ccgggcgatc gccgcggagc cggagctgta cctgctcgac gagccggtga cgggtgtcga
    13201 cgcgaagacc caggaagagc tcatggacgt gctggaggcc gaggcgcggg cgggcaagac
    13261 ggtcatcgcc tcgacccacg acctcatctg cgcggcccag cgcttccatc aggccgcatt
    13321 cgtcaacggc cggatcgtgg cgaccggccc cgccgagatg gtcctcgacc gcaagctcct
    13381 gtccgacacg tacggcggcc acgtcctcgt cctgcccggc gacgacggcg gccggctcat
    13441 cctcgatgac gcccatcacc acgaccaggc gcccggcggc gagtcgcact accacgaagg
    13501 cagtcactga tggacccgat cggcttcctc gcggacccgc tcgcctacgg gttcatgcag
    13561 cgtggcctcg cagcggcggt cctcgtcggg atcgtctgcg cggtcatggg cacgttcgtg
    13621 gttctcaagg gcctcgcgtt catcggcgac gcggtcagcc acgccgcgtt cccgggcctc
    13681 gtgatcgcgt tcatgctcgg gctgccgctc tatctcggcg gtgccgtcgc ctcggtcgga
    13741 accgcgctcg cgatcggctt cgtctcgcga cgcggccggc tccggttcga cacgaccgtc
    13801 ggcgtcctct tcgccgggac gttcgccctc gggatcctgc tgttctcgac gatcaagaac
    13861 tatgtcgccg acctgctcgg ctacctgctc ggcaatgtcc tcgggatcgg gatcggcgac
    13921 ctcatccagg tggccgtgct gggtgccgtc gtgctggtca tcgtcatcgc gatccgcaag
    13981 gagctgctct tcgccacgtt cgatccgctc ggagccgcgg cgtccggact gccagttggg
    14041 gcgctcgagt acctcctcct cgcgctcctc ggcgtgacga tcgtcgtcag catccaggcc
    14101 gtcggcatcg tcatggtcgt cgccatgctg gtcacgcccg cggccaccgc ccagctgctg
    14161 gtcgtgcgct tcgagcggct gatgctgctg gcagcgttgt tcgcgacggt cgccgcagtc
    14221 agcggcctgt acctgagctt ctatgcgaac ctggcctcgg gcgcctcgat cgtcctgatc
    14281 gagacgctgt tcttcctggt cgcgttgctg ttcggcccgc ggagtgggct gctgtcgggc
    14341 cggctggcgc ggcgtcgaag cgagggcctc gagccggcct gagcgacgag cgaaccgcgc
    14401 acccggctgg aacgcatcgt cgacgaaggg agccacccac gtcccagccg gccgcgagca
    14461 tcggcctagc gccgttcgag gcggtacacc ggcgccggcc tgccgttccc gctcggcgcc
    14521 ccgataccga tggtgccggc cggcccttcc atgagggtgt cgggtccgcg ggtcgacgcc
    14581 agcgggagcc aggtcagccc gtccaggctg cggaaccagc ggggcggccg ggtgggcggg
    14641 cacgcggagc cggtgatcga gcacgggtct gttccgagcg cgaggatccc gtcggacgtg
    14701 acgccgagcg ctgagatgga cggcatcggg aaccgcccgc tctgccacgt ctcgagatcc
    14761 gccgacgtcc agatcgtggc gtgcgcgtcc gggccctcct cgccggaagc gacgaccgtc
    14821 cagaacggcg ccggaccgtc cggggcgagg acgcgaatga cctgccagtc gtctccaggg
    14881 ccggtcgaga cgaccggcgg gcccgcgatc gcccgccacg tccggccgtc ggtcgatgtc
    14941 gacaggagtc tcgagcggcc tgcgtcgtca ctcgcgacga cgacccgcgt gccgtcgttc
    15001 gcgacgacga tcaacccgag atccgggttg gccgagatca ttccccggcg ttcccacgtc
    15061 gcggcatcgg ccgactgcca caaccaggtc cggtcgtccg tttccttgag cgccgtctcc
    15121 cagccgctcg gggtcgtcca gacagcggtc gggtaggtga tcggcaagcc cgcgggctcg
    15181 aacgcgtgcg tccgggtcca gtcgagcccg tcgctcgaga tccacgcaac gggtcgcgtc
    15241 tgatcgcacg tctcgccgtg gaggacggtt tccatgccga caaacaccgc gccgccgccc
    15301 gcagcccacc cgtcggccac cgagccatcg ggtcgcgccg tccagcccgg gcaaggtgtc
    15361 actggcgtcg tgaggtccgc ggatcgccag gtgcgcccgt cccgcgagaa cgaggcgacg
    15421 ggagcgaggt ctccgttctg ctcgacccaa cccagggcga ggtagccagc cgagaaccat
    15481 cccttcagga gctcgccgcc tgggtacggc atctcgccga tcttcgtcca cgcgaacggg
    15541 tcgatcgcct cccgggggct cgccgatggg acgtgcgtcg gcgccggatg cgaggtggtc
    15601 acagcgggat gcgaggcggt ggcagccatg gacggggacg gagatccggt gggctgcgcc
    15661 gagggagcag ggttggacgg gagcgggtcc caagacgccg cgatcgacgc gcccggacca
    15721 gctgaagacg gttcgagcgg cgacgacggc ggcgcggagc agccgacgac cacgaagggg
    15781 gcgatcagaa cgatccgccg tagcgtcgat gagacccgca tgacgtgggt cctccgtgcc
    15841 ggggtcgtgg tcgacccgtg gtgccggtca gacgcgcgac gtccgccggt gtttcagcgt
    15901 ctcggcgaac gggtggagcg ctgccgcctg cgtgggcagg cggtcgctac tgctggtagt
    15961 gctcgacgat gtcggccggg cggggcgcgg cgtcgagcgg atccagaccg gcggcccggc
    16021 gggcggcgcg ctggtggagg aggtcgtacg cgcgatcgag ctcgaccctg atcgcctcga
    16081 gccgttc
//
LOCUS       DATAIW010000490         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_138108, whole genome shotgun sequence.
ACCESSION   DATAIW010000490 DATAIW010000000
VERSION     DATAIW010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138108"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VHM48_14505"
     CDS             <1..1526
                     /locus_tag="VHM48_14505"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2756677.1"
                     /translation="GDELPTAARTIDAPGKVVAPGFIDLHSHAALMILADPDHGAKAR
                     QGVTTELVGVDGLSYVPFPNAADLAAMVVMNAGLDGDPRDDDGARGTSGRGRPVIIDW
                     ATVEDVLARYDTGTAVNVAQLVGNNALRIAALGWDDVPTNARATSDQRARLRAAMEEG
                     AFGVSSGLDYPPGAYATTAELGDLANEAARLGGMYHTHVRYPLGDGYLDPFREAIEIG
                     RRGGAPVHITHFYHRLTFPGPPEQMLQLVDDAVAAGQDVTFDLYPSEWASSRLLILIP
                     LWVLEGGPTRTKERLADRQVRDEIRRDLAARGELFAGSGGLRDIRIGYLRRPENLPWE
                     GRTLGELIDAAGNDPVDTLCDLLLAEDLRPNEVTPGPNVEGIRTFLRHPRAMIGTDSV
                     FLGARPSPRTYGTYPRILGQFVRDEALLGLEDAVARMTSLPAARLGLRDRGTIRDGAV
                     ADLVIFDPATVRSDATIDDPTRDPEGIDTVIVAGTPVVDGGRPTGARPGRGLRRGRD"
BASE COUNT          242 a          652 c          589 g          209 t
ORIGIN      
        1 cgggcgatga gctcccgaca gccgcccgca cgatcgacgc ccccggcaag gtcgtggccc
       61 cgggcttcat cgacctccac tcccacgccg cgctgatgat cctcgccgac ccggaccacg
      121 gggcgaaggc ccgccagggc gtcacgaccg agctggtcgg ggtcgacggc ctcagctacg
      181 tcccgttccc gaacgccgcc gacctcgccg cgatggtcgt gatgaacgcc ggcctcgacg
      241 gcgacccgcg ggacgacgac ggcgcccgag gaacgagcgg gcgcggccgc ccggtgatca
      301 tcgactgggc gaccgtcgag gatgtcctgg cccgctacga caccggcacc gccgtgaacg
      361 tggcccagct cgtcgggaac aacgccctcc ggatcgccgc tctcggctgg gacgacgtgc
      421 cgacgaacgc ccgcgcgacg agcgaccagc gggcccgcct gcgggcggcc atggaggagg
      481 gtgcgttcgg agtcagcagc ggcctcgact acccaccggg cgcctacgcc acgaccgcgg
      541 agctcggcga ccttgccaac gaggcggccc ggctcggcgg gatgtaccac acccacgtcc
      601 ggtacccgct cggcgacggg tacctcgatc cgttccgcga ggccatcgag atcgggcggc
      661 gcggcggggc gcccgtccac atcacccatt tctatcaccg cctcacgttc ccgggaccac
      721 ccgagcagat gctccagctc gtcgacgacg cggtcgcggc gggccaggac gtcaccttcg
      781 acctctaccc gtcggagtgg gcgagcagcc gcctgctcat cctcatcccg ctctgggtcc
      841 tcgagggcgg gccgaccagg acgaaggagc gcctcgccga ccggcaggtc cgcgacgaga
      901 tccggcgcga cctcgccgcc cgcggcgagc tcttcgccgg cagcggcggc ctccgcgaca
      961 tccggatcgg ctacctccgc cggccggaga acctcccgtg ggagggtcgg acgctcggcg
     1021 agctgatcga tgcggccggc aacgaccccg tcgacacgct ctgcgacctg ctcctcgccg
     1081 aggacctccg gccgaacgag gtcacgccgg gcccgaacgt cgagggaatc cggaccttcc
     1141 tccgtcaccc gcgggcgatg atcgggaccg acagcgtctt tctcggcgcc cggccgagcc
     1201 cccggacgta cggcacgtat ccccggatcc tcggccagtt cgtgcgcgac gaggcactgc
     1261 tcgggctcga ggacgccgtc gcgaggatga cctcgctgcc cgccgcccgg cttggcctgc
     1321 gcgatcgcgg cacgatccgc gacggcgcgg tcgccgacct cgtgatcttc gatccggcga
     1381 ccgtccgcag cgacgccacg atcgacgacc cgacccgcga tcccgagggc atcgacaccg
     1441 tgatcgtcgc gggcaccccg gtcgtcgacg gcggtcggcc gacgggtgcc cgacccggtc
     1501 gcggtctgcg ccgcgggcgc gactgacgat cgggggccag gccctcagcg gcgaggtcga
     1561 ggtcatcggt gaggaggggg gctcgatccg ggttcgcacg cccgatggca tcgagggatg
     1621 ggtgccgcgc gtggtcatcg tcggatcacc tgcgcgggaa cgagccccgt cggcatctca
     1681 accggagccg cc
//
LOCUS       DATAIW010000491         9104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_232274, whole genome shotgun sequence.
ACCESSION   DATAIW010000491 DATAIW010000000
VERSION     DATAIW010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232274"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(637..2049)
                     /gene="cysS"
                     /locus_tag="VHM48_14510"
     CDS             complement(637..2049)
                     /gene="cysS"
                     /locus_tag="VHM48_14510"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2756678.1"
                     /translation="MTIRLHDTLTGEDQPLEPLEPGLVRIYSCGPTVYGPAHIGNFRS
                     FLFADILVRHLRRRGLRVTWVMNITDIDDKIIKGAAAAGTSIQELTDRWLAAFRIDAD
                     RLRMTPPDVQPRATEHIDDIVALIATLLERGHAYRTDDGSIYFRIASWPAYGQLARLD
                     PEQMRVGERVESDEYAKDDVRDFAVWKGAKAGEPSWDTAIGPGRPGWHIECSAMSMKQ
                     LGPSFDIHTGGVDLVFPHHEDEIAQSEAATGQRFVRTWLHCAHLQSSGSKMAKSTGNI
                     ARVGDLLDAGVSARALRYALIAVHYRAPLNYSDESLPAATAALDRLEAAVAALTAYRE
                     ERLDDPELDGVLGVAADAFDAALDDDLNISAALAVVFDLVRDLNRRIDGRVLSTADAE
                     RALTVIRSFDEVLGVLPAADENLDEESRRMLDERATARSNRDWAASDRLRDELAERGI
                     AVEDTRDGQRWRRQLEAARG"
     gene            complement(2046..3305)
                     /gene="ispD"
                     /locus_tag="VHM48_14515"
     CDS             complement(2046..3305)
                     /gene="ispD"
                     /locus_tag="VHM48_14515"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX2756679.1"
                     /translation="MTIEDEPPFADAIVVAAGESSRMGGIDKVTAVVGGLPLLAHAVV
                     AIAAAPEVRRIVIVTSADRIEEVAAADWLPPTVLDPVTGGPRRQESVQRGLDQLDERA
                     AQDGGDAAVILVHDGARPLVPTALVSAVARAARIHGAAIPVLPVGDTIKRVEPDGSVA
                     GAGDRSTLGAAQTPQGIRSDLLHDAFARFPPAGRDLFTDEAALLEACRIPVHAIPGDE
                     NNFKVTLPADLARVETALAGAHPGVTARRADGDDGGDGAGRRIGFGSDSHSFGPGTGL
                     ALGGFVVERAPRLHGHSDGDVVLHAVADALLGAAGLGDLGRIFPAGPETPRGIASAEL
                     LAEVLRRVSAAGYAVANLDCTITASRPRLGGLLPSIGARIADLLALDPSAVNVKASSG
                     NLAGMEGAGRGISATAVVILARPPLDR"
     gene            complement(3373..4431)
                     /locus_tag="VHM48_14520"
     CDS             complement(3373..4431)
                     /locus_tag="VHM48_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2756680.1"
                     /translation="MIRNIRLLGAALGGLVGLALAASARLFEESPTGGPLLVAWVVAW
                     IVVGFAILPYLTVVPAGWLIRQAQQLSTAEFVTAVVGLLIGLLMGLLLGLPLALFPAP
                     FGNWLPIGVSLFLGLGMLGLTVAKRDDLLVAAEAIGLVRRPRPEGDREAPTGDPRIVV
                     DTSAIIDGRIKDIVDSGFVYGTLVVPKFVLEELQHIADSSDTLRRNRGRRGLEILAQM
                     QKDSPTPIEIVDEPLPDIAEVDAKLVALARARSKAILTNDYNLNRVAELQGVRVLNVN
                     SLANAVKPAVLPGEELRVRVIQEGKEAGQGVGFLDDGTMIVVEGGVRYIDKDLDVSVT
                     RVLQTVAGRMIFAQPRLD"
     gene            complement(4502..5956)
                     /gene="radA"
                     /locus_tag="VHM48_14525"
     CDS             complement(4502..5956)
                     /gene="radA"
                     /locus_tag="VHM48_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX2756681.1"
                     /translation="MSKASGRAQTRFVCQSCGESFLRWEGQCRACSAWNSLVETQVRT
                     EPRPSRLVVGGGLAAPVATRLIDVGEPERPRLPVGIGELDRVLGGGFVPGSVVLLGGE
                     PGIGKSTLLLQAAAGIAGLVGAERVLYATGEESGAQVRLRAERLGILGGTGGAIRVLA
                     ESSIDRIAELVSAGLPKPALLVIDSVQTATVDDLDGPAGSVGQVRGAALRLMELAKPA
                     GIAVVLVGHVTKDGSIAGPKTLEHLVDAVLTLEGERYATVRLLRASKNRFGSTEEVGV
                     FEMGEAGMTEVADPARAFLAEHDGPAPGSIVAPTLEGSRPLLVEVQGLVAPGPGGTPR
                     RTASGVDTNRVALLVAVLARRAGVGLASHDIYVNLAGGLAVDEPGLDLPLALALASSL
                     RDRPIATGTVAIGEVGLLGELRSVVGLERRLREAARLGFGRAIVPRPGRGPVTPVIPG
                     MEVIAVGSLREAIEAALSTTGGVSRRMAATSARC"
     gene            complement(5982..8453)
                     /locus_tag="VHM48_14530"
     CDS             complement(5982..8453)
                     /locus_tag="VHM48_14530"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2756682.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELAKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHEVIRVLSQSSSAGPAQETKRAS
                     KTPTVDQLGINLTELARQGKLDPVIGRDKEIERVIQILSRRTKNNPALIGEPGVGKTA
                     IAEGLAHRIVSGDVPETLLNKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRNTNDA
                     VLFIDELHTLVGAGAAEGAIDAANILKPPLARGELQCIGATTLDEYRKYIERDAALER
                     RFQPVMVEEPTLEQTIDILMGIRERYEQHHKVTITDDAVKAAADLSTRYITDRHLPDK
                     AIDLIDEAASRVRLRHASAPPALREAQKVLDRVTKEKDAAINNQEYEEAARLREAEAT
                     SREGVDKLRAEWQAQVSSEQPTVDEEEIAQVVAMWTGIPVTRIAAEESERLLHMEDAL
                     HSRVIGQQEAIEVVSKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELAKALAEFMFG
                     SEDALIKIDMSEFMERHNVSRLVGAPPGYVGFDEGGQLTEAVRRKNYCVVLLDEVEKA
                     HPEVFNILLQILEDGHLSDAKGRRVDFRNVIIIMTSNLGAKQLQTNSSLGFRDRGGSA
                     ESQAQTSYDLMKEKIAAELKNNFRPEFLNRIDATVVFRSLTVEEITLIVDLMLARVRD
                     QLRAQQMLLEVTQAAKEHIIKLGYDVAYGARPLRRVIQNMVEDVLAEHLLLGKYEPGT
                     TIVVDRDPEAGLDIHAAEPLTPVEA"
     gene            complement(8711..>9104)
                     /locus_tag="VHM48_14535"
     CDS             complement(8711..>9104)
                     /locus_tag="VHM48_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756683.1"
                     /translation="AEPSASSSANAPSSPSAPRGERRPRPERGWSASDAVQEPEGGID
                     NTLAAAFALVRDQLAASEEAQTAEPEAVAEVAEPEAVATEPEAVATEPEAVATEPEAV
                     ADPDEEATAPDDEAEPGTESNDEESTPA"
BASE COUNT         1305 a         3172 c         3268 g         1359 t
ORIGIN      
        1 tgacacccgc cctgtcgacg gttcacgcga gcgttcgtgg gatcgcccgg ctcccccatg
       61 ggccggccag ccggtccgct cggtcgtggc cgatcggcac cgccgcgtga tccgccggcc
      121 catgggggag ccgggcgatc ccacgaacgc tcgcgtgaac cgtcgacagg gcgggtgtca
      181 tcaccgcggc ggtcaagatc tggacccgtc gcgtcgggac ggacgccgcg gaatggcggc
      241 cgcggcccgg atcgaccgcc gaaaccaccc gactgcccgc cgcgatcgaa cgaccgatcc
      301 gcgggcggcc cgcttcggcg tggaccgaac gaccgttcgc cgcgttcgcc gccgcccgac
      361 tcgcggtctc cgccgccggc accggcgccg ccgccgaacc cgaagccggg ccgctggccg
      421 ggaccggagc ggtccgctcc tcccggcccg ccccggctcg atcctccgcg gaagccgccg
      481 cccggaccgc tccggaagcc cgggccgccg ggaccgccac gcgaggtacc tccgccccgc
      541 gaggtacctc cgcctcgcgg tccggaatga ccgccggagc ccttcggacc tccgtggcgc
      601 ttgggccgct cgccatcgcc cccggaattc ggcctgtcag ccacgagctg cctccagctg
      661 gcgacgccag cgctgaccgt cgcgcgtgtc ctcgaccgcg atcccgcgct cggcgagctc
      721 gtcgcgaagg cggtcggacg cggcccagtc gcggttggag cgggcggtcg cccgttcgtc
      781 gagcatgcgg cggctctcct cgtcgaggtt ctcgtcggcc gccggcagga cgccgagcac
      841 ctcgtcgaac gagcggatga cggtcagcgc ccgctcggcg tcggccgtgg acaggacccg
      901 gccgtcgatc cgtcgattga gatcgcggac caggtcgaag acgaccgcga gggccgccga
      961 gatgttgagg tcgtcgtcga gggcagcgtc gaacgcgtcg gcggcgacgc ccaggacgcc
     1021 gtcgagctcg gggtcgtcga gccgttcctc ccggtatgcc gtcaaggccg ccacggccgc
     1081 ctcgagtcga tccagggcgg cggtggcagc cggcagcgac tcgtcgctgt agttcagcgg
     1141 cgcccggtag tggacggcga tcaacgcata gcggagggcg cgggccgaga cgccggcgtc
     1201 gagcaggtcg ccgacgcggg cgatgttgcc ggtcgacttg gccatcttcg acccgctcga
     1261 ctggagatgg gcgcagtgaa gccaggtccg aacgaaccgc tggccggtgg ccgcctcgct
     1321 ctgggcgatc tcgtcctcgt ggtggggaaa gaccaggtcg acgccgccgg tgtggatgtc
     1381 gaatgacggc ccgagttgct tcatcgacat cgccgagcac tcgatgtgcc agccgggccg
     1441 ccccgggccg atcgcggtgt cccacgacgg ctcgccggcc ttggcgccct tccagaccgc
     1501 gaaatcgcga acgtcgtcct tggcgtactc gtcggactcg acccgctcgc caacgcgcat
     1561 ctgttccggg tccaggcggg cgagctggcc gtaggccggc caggacgcga tccggaagta
     1621 gatcgagccg tcgtcggtcc gataggcgtg accgcgctcg agcagcgtcg cgatcagggc
     1681 cacgatgtcg tcgatgtgct cggtcgctcg cggctggacg tccggtggcg tcatccgcag
     1741 gcggtcggca tcaatccgga aggcggccag ccagcgatcg gtcaactcct ggatcgacgt
     1801 gcccgcagca gcggcgccct tgatgatctt gtcgtcgatg tcggtgatgt tcatgaccca
     1861 ggtgacgcgg aggcctcgcc gccgcaggtg gcgaacgagg atgtccgcga acaggaagct
     1921 ccggaagttg ccgatgtgcg ccggcccata gaccgtcggt ccgcagctgt agatccggac
     1981 gagacccggc tcgagtggct cgagcggttg gtcctcgccc gtgagggtgt cgtggagccg
     2041 gatcgtcatc ggtcgagcgg tgggcgggcg aggatcacga ccgcggtcgc cgagatgccg
     2101 cgacccgcgc cctccatgcc ggcaaggttg ccgctggagg ccttgacgtt gacggcggat
     2161 ggatcgaggg cgagcaggtc ggcgatccgg gctccgatcg acgggagaag cccaccgagg
     2221 cgcggccggg acgcggtgat cgtgcaatcc aggttggcca ccgcgtagcc ggccgcgctg
     2281 acgcggcgga ggacctctgc caggagctcc gcgctggcaa tgccccgcgg cgtctcgggt
     2341 ccggccggga agatccggcc gaggtcaccc agcccggccg cgccgagcag ggcatcggcg
     2401 accgcgtgga ggacgacgtc gccgtccgag tggccgtgca accggggtgc gcgctcgacg
     2461 acgaagccac cgagagcgag cccggtcccg ggcccgaagg agtggctgtc ggacccgaaa
     2521 ccgatgcgcc ggccggcgcc gtcaccgccg tcatcgccgt cggcgcggcg ggccgtgaca
     2581 cccgggtggg caccggccag ggccgtctcg acacgcgcca gatcggccgg gagggtcacc
     2641 ttgaagttgt tctcgtcgcc ggggattgca tgcaccggga ttctacaggc ctcgagcagg
     2701 gcggcctcat cggtgaacag gtcacgtccg gccggcggga accgggcgaa ggcgtcgtgg
     2761 agcaggtcgg accggattcc ctggggggtc tgggcggcgc caagcgtcga tcgatcgccg
     2821 gcgccggcga cggagccgtc tggttcgaca cgcttgatcg tgtcgccgac gggcagcacc
     2881 gggatcgccg cgccgtggat gcgcgccgcc cgggcgaccg ccgacacgag ggccgtcggg
     2941 acaagcggcc gggcgccgtc gtgaacgagg atcaccgcgg cgtcgccgcc gtcctgggcc
     3001 gcccgttcgt ccagctggtc gagaccgcgc tggacggact cctgccgccg gggcccgccc
     3061 gtcaccgggt ccagcacggt cggcgggagc cagtcggcgg cggcgacctc ctcgatccga
     3121 tcggcactgg tcacgatgac gatccgtcgg acctccggcg ccgccgcgat cgcgacgacg
     3181 gcgtgcgcca ggagcggcag gccgccgacg acggccgtga ccttgtcgat cccgcccatc
     3241 cgactcgact ccccggcggc gacgacgatc gcgtcggcga agggcggctc gtcctcgatc
     3301 gtcaaaccgc caccctcaac agcgatctac gactcgactc cgaggtccga gggcccgagg
     3361 attgtgaacg tctcaatcga gtcgcggctg ggcgaagatc atccggccgg cgaccgtctg
     3421 gagcacgcgc gtgaccgaca cgtcgagatc cttgtcgatg tagcggacgc cgccttcgac
     3481 cacgatcatc gttccgtcgt cgaggaaccc gaccccctgg ccggcctcct tgccctcctg
     3541 gatgacccgg acccggagct cctcgccggg gagaaccgcg ggcttgaccg cgttcgccag
     3601 ggagttcacg ttcagcaccc ggacgccctg caactcggcg acgcgattga ggttgtagtc
     3661 gttcgtcagg atcgccttgc tccgggcgcg ggcgagggcg acgagcttgg catcgacctc
     3721 ggcgatgtcc gggagcggct catcgacgat ctcgatcggg gtcggcgaat ccttctgcat
     3781 ctgggcgagg atctcgagac cgcgccgccc gcggttgcgg cgcagggtgt cggagctgtc
     3841 ggcgatgtgc tgcagctcct cgaggacgaa cttcggaacg accagcgttc cgtagacgaa
     3901 ccccgagtcg acgatgtcct tgatccggcc gtcgatgatc gcgctcgtgt cgaccacgat
     3961 gcgcgggtcg ccggtcggtg cctcgcgatc gccctccggc ctcggccgcc gaaccaggcc
     4021 gatcgcctcg gcggcgacga gcagatcgtc gcgcttggcg accgtcagac cgagcatgcc
     4081 cagaccgagg aacagggaga cgccgatcgg cagccagttc ccgaatggag ccgggaacag
     4141 cgccagcgga agcccgagga ggaggcccat caacaggccg atcaggagcc cgaccaccgc
     4201 cgtcacgaac tcggcggtcg agagctgctg ggcctgacgg atcagccatc cggccgggac
     4261 gacagtgaga tacggcagga tcgcgaagcc gaccacgatc caggccacca cccaggcgac
     4321 cagcagcgga ccaccggtcg gcgactcttc gaacagtcgg gccgaggccg ccagggcgag
     4381 cccgacgagg ccacccaggg ctgccccgag caagcggatg ttgcggatca cgttttcctc
     4441 gcgtcgtccc tcgcggggcg aaggcgccac acgtcccggg gcgctctgaa gccgccgtag
     4501 cctagcatcg cgccgaggtc gccgccatcc gacgcgagac accacccgtc gtcgacaggg
     4561 cagcttcgat cgcttcgcgg aggctgccga cagcgatgac ctccatcccg gggataaccg
     4621 gggtcactgg gccccggccg ggcctcggga cgatcgcccg gccgaagccc aggcgagcgg
     4681 cctcgcgcag gcggcgctcc agccccacga ccgaccggag ctcgccgagc aggccgacct
     4741 cgccgatcgc gaccgtgccc gtggcgatcg ggcggtcgcg cagtgacgac gcgagggcaa
     4801 gggccagcgg caggtcgaga cccggctcgt cgaccgcaag cccaccggcg agattgacgt
     4861 agatgtcgtg gctggcgagg ccgacgcccg cgcgacgggc aaggacggcg acgagcaggg
     4921 cgacgcggtt ggtatcgacg ccgctggcgg tccgtcgcgg cgtcccaccc gggcccggtg
     4981 cgacgaggcc ctggacctcg acgagcaagg gccgcgatcc ctcgagggtc ggagcgacga
     5041 tgctgccggg tgcggggccg tcatgttcgg cgaggaacgc tcgagccgga tcggccacct
     5101 cggtcatgcc ggcctcgccc atctcgaaca cgccgacctc ctcggtcgag ccgaagcggt
     5161 tcttcgacgc ccgcagcaag cggacggtcg cgtaccgctc gccctcgagg gtcagcacgg
     5221 cgtcgacgag gtgctcgagc gtcttcggtc cggcgatcga gccgtccttg gtcacgtgtc
     5281 cgacgaggac gacggcgatc ccggccggct tggcgagctc catcagccgg agcgccgcgc
     5341 cgcggacctg gccgacgctc ccggccgggc cgtcgaggtc gtcgaccgtc gcggtctgga
     5401 ccgagtcgat gacgaggaga gccggcttcg gcaggccggc cgagaccagc tctgcgatcc
     5461 ggtcgatcga cgattcggcc aggactcgga tggcaccgcc agtgccgccg agaatgccga
     5521 ggcgctctgc acgaaggcgg acctgggccc ccgattcctc gccggtcgcg tagaggacgc
     5581 gctcggcgcc cacgaggccg gcgatccccg cggccgcctg gagcaggagg gtcgacttgc
     5641 cgatcccggg ctcaccgccg aggaggacga ccgaaccggg gacgaatcca ccgccgagga
     5701 cgcggtccag ctcgccgatc ccgaccggca agcgcggccg ctccggctca cccacgtcga
     5761 tcaggcgggt ggcgaccggt gccgccagac cgccaccgac gaccaggcgc gacggccgcg
     5821 gctcggtccg gacctgggtc tcgacgaggc tgttccaggc ggagcaggcg cgacattgcc
     5881 cttcccagcg gaggaacgat tcgccgcagg actggcagac gaagcgggtc tgtgcacgac
     5941 cggacgcctt gctcatggcg agcggtcccg gcgcccgatg gtcaggcttc gaccggggtc
     6001 agcggctcgg cggcgtgaat gtcgaggccg gcttccgggt cgcgatcgac cacgatcgtc
     6061 gtgcccggct cgtacttgcc gagcaggaga tgctcggcga ggacgtcctc gaccatgttc
     6121 tggatgactc ggcgaagcgg ccgggcgccg taggccacgt catatccgag cttgatgatg
     6181 tgttccttgg ccgcctgggt gacctcgagg agcatctgct gggcgcgcaa ctggtcgcgg
     6241 acccgggcga gcatgagatc gacgatcagg gtgatctcct cgaccgtcag cgaccggaac
     6301 acgaccgtcg cgtcgatccg gttgaggaac tccggccgga agttgttctt cagctccgcc
     6361 gcgatcttct ctttcatgag gtcgtacgac gtctgggcct ggctttcggc gctgccgccg
     6421 cgatcccgga acccgagcga cgaattcgtc tggagctgct tcgcgccgag gttcgaggtc
     6481 atgatgatga tcacgttgcg gaagtcgacc cggcggccct tggcgtcgga gagatggccg
     6541 tcctcgagga tctggaggag gatgttgaac acctcggggt gggccttctc gacctcgtcg
     6601 aggaggacga cgcagtagtt cttgcggcgg accgcttcgg tcagctggcc gccttcgtcg
     6661 aagccgacgt agccgggggg cgctccgacc aggcgcgaga cgttgtggcg ctccatgaac
     6721 tcgctcatgt cgatcttgat gagcgcgtcc tcgctgccga acatgaactc ggcgagggcc
     6781 ttcgcgagct cggtcttccc gacgccggtc ggcccgagga agatgaacga gccgatcggg
     6841 cgcttcggat ccttcaggcc ggcccgggcg cgacggacgg ccttcgagac gacctcgatc
     6901 gcctcctgct ggccgatgac ccggctgtgg agggcgtcct ccatgtggag gaggcgctcc
     6961 gattcctcgg ccgcgatccg ggtgaccgga attccggtcc acatcgccac gacctgggcg
     7021 atctcctcct cgtcgaccgt cggctgctcg ctcgagacct gcgcctgcca ctcggcgcgg
     7081 agcttgtcga cgccctcacg ggacgtggcc tcggcctcgc gcaggcgggc agcctcctcg
     7141 tactcctggt tgttgatcgc ggcgtccttc tccttggtca cccgatcgag gaccttctgg
     7201 gcctcgcgca gggcaggcgg tgcggacgca tggcgaaggc gcacgcgtga ggctgcctcg
     7261 tcgatcaggt cgatcgcctt gtcgggaagg tggcgatcgg tgatgtagcg ggtcgacagg
     7321 tccgcggcgg ccttgacggc gtcgtcggtg atcgtgacct tgtggtgctg ctcgtagcgc
     7381 tcgcggatcc ccatgaggat gtcaatcgtc tgctcgagcg tcggctcctc gaccatcacg
     7441 ggctggaagc gtcgctcgag tgccgcgtcg cgctcgatgt acttgcggta ctcgtcgagg
     7501 gtcgtggcac cgatgcactg gagctcgccg cgggcgagcg gcggcttgag gatgttggcg
     7561 gcgtcgatcg caccctcggc cgcgccggca ccgacgagcg tatggagctc atcgatgaag
     7621 agcacggcgt cgttggtgtt gcggagctcc tcgatgatct tcttgaggcg ctcctcgaat
     7681 tcgccgcggt acttggtgcc cgcgaccagc gaaccgatgt cgagcgtcag gacccgcttg
     7741 ttcaagaggg tctcggggac gtcacccgag acgatccgat gagccaggcc ctcggcgatc
     7801 gccgtcttcc cgacgcctgg ctcaccgatc agggccgggt tgttcttggt ccgccgcgaa
     7861 aggatctgga tgacccgttc gatctccttg tcccggccga tcaccgggtc gagcttgccc
     7921 tgacgagcga gctcggtgag gttgatcccg agctggtcga ccgtcggcgt cttgctggcg
     7981 cgcttcgtct cctgggccgg gccggccgac gagctctgcg acaggacgcg gatcacctcg
     8041 tggcggacct tgtcgaggtt cacgccgagc gactcgagga ctccggcggc gatcccctcg
     8101 ccctcccgga cgagacccag aaggaggtgc tcggtcccga tgtagttgtg gccgagtcgg
     8161 cgcgcttcgt cgatcgcgag ctcgatgacg cgcttcgctc gaggcgtcag gccgacttca
     8221 ccgacgaccg gccggtcgcc gcggccgatg atgaattcaa cggcggtccg gaccttggcc
     8281 agctcgacgt tcatgttctc gaggacgcgc gccgcgactc cctcgccctc gcggacgagg
     8341 cccaggagca ggtgctccgt gccgatgtag ttgtggttga agcgctgcgc ttcgtcctga
     8401 gcaagcgtca ggaccttgcg ggcgcgatcc gtgaacttgt cgaagcgatc catcgagtcg
     8461 aacgttcgtt cccttgcgag cgtctcaggg ggcgctccga aagccatgct agcacggacc
     8521 ccttcgcccg caagccagtc gaacgggcct ccccggatag gtccgtcgac gggtccttga
     8581 ccggcccggt cctgcccaat cattcgccgg atccgccacg gcagatgcgg gattgcgggc
     8641 gcgatttggt gaaggaatcg aaacggcccg ccgtcggcgg accgttcgcg atcccggggt
     8701 cgagccgcgg tcaggcgggc gtcgactcct cgtcgttcga ttcggtcccg ggctcggcct
     8761 cgtcgtccgg agccgtggcc tcctcgtccg gatcggcgac agcctccggc tcggtggcga
     8821 cggcctccgg ctcggtggcg acggcctccg gctcggtggc gacggcctcc ggctcggcga
     8881 cctcggcgac ggcctccggc tcggcggtct gagcctcttc cgaagcggca agctgatccc
     8941 ggaccagggc gaatgccgcg gccagggtgt tgtcgatgcc gccctcgggc tcctggacgg
     9001 cgtccgacgc cgaccagccg cgctccgggc gcggccgccg ctcgccgcgc ggggcggagg
     9061 gtgacgacgg cgcgttcgcg ctcgagctcg cgctcggctc ggcc
//
LOCUS       DATAIW010000492         6753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_202564, whole genome shotgun sequence.
ACCESSION   DATAIW010000492 DATAIW010000000
VERSION     DATAIW010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202564"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VHM48_14540"
     CDS             complement(<1..812)
                     /locus_tag="VHM48_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX2756684.1"
                     /translation="MTRSRINDPVDALGLYGPGSEAWTLNREAMLLLGAGPRALLMQL
                     AHPQVAAGVADHSDFRADPWARLDGTLRSYLRIVYGTRTAAHAEIRRLNELHRTITGP
                     TYRARDPDLSLWVHATLVDSTIVAADRWLEPLSRERRARYYAETVPIGRAFGIPADLL
                     PADLEAFEAYVAASLAPGGPVHVGAVARELADVVLHPPLGPVAPPLAGLPTWTYAWTL
                     WPAVGLLPSTIRTEYGMRWGSFERAVSAWLVGTWRAWRAILPPGFRQMPQALA"
     gene            829..1410
                     /locus_tag="VHM48_14545"
     CDS             829..1410
                     /locus_tag="VHM48_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756685.1"
                     /translation="MGRWGAEFDPDRLADLETAMWKAYYRRQPARLFGLLIQGLREQA
                     HVSWPRALAAAFFLTKGAAGFARAAGDYERFAPDIARGYRLLGLPPDLDAVEVGRREL
                     RWWVVRREIGLAAGAAAGEAITRLYAAIYALPESRVADAGRLRGEAAEVRDRGATEDP
                     AGPAARGLAYWPEVARLLRQSYADLHRALAEGN"
     gene            1487..2455
                     /locus_tag="VHM48_14550"
     CDS             1487..2455
                     /locus_tag="VHM48_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX2756686.1"
                     /translation="MPLFGVDVSRHQGPDINWGKVRASGIDFMLARASLATTPDATYR
                     SNVRRARLAGIPVVGAYHFLYPAKVVSPAEQARLFVDRIGNADGILTMLDVEKDRNPK
                     TGQVFIPTISDVRAFAREFARLTHNHTLIVYAPGWYWTGHIGNPPASDLGPLCASRYV
                     PVRHDHEGHPIAMTPKEAFAKVTPKFWNPTHGGWKKATILQFTSTGRIGGYGGPVDVD
                     AFNGTLQKLKSLTGPVGHAVSALVPPASTGPAPDHGKPIVVKPTTKRSHVVVAGETLS
                     GIAARHGFNATAKLPTFRVMLNAFPENAKFRANPHVIRPGDRVRVR"
     gene            complement(2477..3121)
                     /locus_tag="VHM48_14555"
     CDS             complement(2477..3121)
                     /locus_tag="VHM48_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2756687.1"
                     /translation="MISASARLSRLPVEERGLTIGDFLVPIRIGERMGSRVRHIALIA
                     IGATLVFASSRVSIPIPGSDVPITGQTFGVLLVGGALGFRRGLASVGLYVLIGLIGLP
                     FFAESKGGVSVILGASGGYIVGFLVAGSLVGRLAELGWDRRILGALGAMAIGNVVIYV
                     IGVPWYMVVAHLDLPTGIAKGLTPFLLVDAIKLILAAVTFPAAWWVVGRRAGEG"
     gene            complement(3224..5737)
                     /locus_tag="VHM48_14560"
     CDS             complement(3224..5737)
                     /locus_tag="VHM48_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2756688.1"
                     /translation="MSSLAIRSLAARRGRTSLSIVGIALGIAVLYASLATDAGIAASI
                     DRTVRDLVGRADLRVESFGPAGLSPESLAAIEAAPGVARAAPALQRRTYLVPAIDDPN
                     AAPPPVTVLGIDPGPEAAVRDLVLASGASLAGPDGFQALITQTLATANGTVVGGTITV
                     PASDGAPIDLTVVGILAGDGPIVGSAGRTVILPLGTMQRILADDTVSRVDIVAGEGAT
                     PTEVASAIDVALTSQPYVISSPADVAASLRGSTADFRSTTALIAAVALFVGAFLIFNT
                     LSMTVAERVRELGLLRAAGATRRQLVAFVAAQAVVLGLLGALVGLVAGLVLAELMAAD
                     LRSIAAIPFERVDPSLASIAAVIAIGLGVTVAAAIEPARRAGSIPPVEALRDRIDSSA
                     ARRARLRWLVGVFVVVGIAGLLAWPRDAGTAGLARSGLVYLVLFGAVLAAPFALGVLG
                     RLAGLPFRGVLRVEERLARAAIVRDRSRTTLTVGALAIGLAMVVAVGSVAHQSRLAAS
                     AWLDEVIPGDELVTSIRPIPLDEPVIADLAAVDGVARVSPFAIFEVARNGVRLDAAAV
                     DGADLLADGRLRFVDGDRTAALQGLDAGGVAVLPRSIANRTGLTVGSTLTLAVGEGQV
                     VDLRVAGITERTLPGGVGEAVLVGWPDATAVFGVQGADVLAVRFTPGRAAEARPRLEA
                     AARNAALEPNPLETVAGAVDDALSQVFGLFDALALVAVVVAALGIVNTLTVSVLERVR
                     ELGVLRAAGMTRGQVRRTVVVEAGILGIVGSILGVVTGLVAGAILVLLAGGGPLILEV
                     PWTSIGVAIVLGIGISMAAAWYPARLASRLAIVAAVQHE"
     gene            complement(5750..6502)
                     /locus_tag="VHM48_14565"
     CDS             complement(5750..6502)
                     /locus_tag="VHM48_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756689.1"
                     /translation="MAAILEAHDLAKRYRLGATTVDALRGVSLTVDEGEFVALMGPSG
                     SGKSTLLQLLGGLDRPSSGEVVLQGETISQLSDDAATKLRRDRTGFVFQSFNLIPLLD
                     VTENVALPFTIAGADTGKGEIAERIRDVIALVELAGKERHKPDQLSAGEQQRVAIARA
                     LVTRPALLFADEPTGNLDYTTGTEILEALWRSCVERHQTVVLVTHDSKAAAYADRVLV
                     ISDGRIRDEIVLGRRAEHDTGPLVSRLAELGL"
BASE COUNT         1044 a         2413 c         2486 g          810 t
ORIGIN      
        1 gcgagcgcct ggggcatctg gcggaagccc ggcggcagga tcgcgcgcca cgcccgccag
       61 gtgccgacca gccaggccga gacggcccgc tcgaacgagc cccagcgcat gccgtactcg
      121 gtccgaatcg tcgacggcag caggccgacc gccggccaca gcgtccaggc atacgtccat
      181 gtcggaagcc cggcgagtgg cggtgccacc ggcccgagcg ggggatgcag cacgacgtcc
      241 gcgagctcgc gggcgaccgc gccgacatgc acgggaccgc cgggcgcgag cgaggccgcg
      301 acgtacgcct cgaacgcctc gagatccgcc gggagcaggt ccgcggggat cccgaacgcc
      361 cgcccgatcg gcaccgtctc cgcgtagtaa cgggcgcgcc gctccctgga cagcggctcg
      421 agccagcggt cggcggcgac gatcgtcgaa tcgaccagcg tggcgtggac ccacagcgag
      481 agatcggggt cgcgggcgcg gtaggtcgga ccggtgatcg tccggtggag ctcgttcagg
      541 cggcggatct ccgcgtgtgc cgccgtccgc gtcccgtaga cgatccggag atagctccgc
      601 agcgtgccgt cgagccgggc ccaggggtcc gcccggaagt cgctgtggtc ggcgacgccg
      661 gcggccacct gcgggtgggc gagctgcatc agcagggccc gcgggcccgc cccgagcagg
      721 agcatcgcct cgcggttgag ggtccacgcc tccgaccccg ggccgtacag tccgagggcg
      781 tcgacgggat cgttgatccg tgaccgcgtc atctgcgaag gatcgggcat gggacgctgg
      841 ggcgcagaat tcgacccgga ccggctcgcg gacctcgaaa ccgcgatgtg gaaggcctac
      901 taccgacgcc agccggcgcg gctgttcggg ctgctgatcc agggccttcg cgaacaggcc
      961 cacgtctcct ggccgcgggc cctcgcggcg gcgttcttcc tgacgaaggg ggccgcgggg
     1021 ttcgcccggg cggccggcga ctacgagcgg ttcgcacccg acatcgcgcg cggctatcga
     1081 ctcctcgggt tgccgccgga cttggacgcg gtcgaggtcg gccggcgcga gttgcgctgg
     1141 tgggtcgtgc ggcgcgagat cgggctggcg gccggtgccg ccgcgggcga ggcgatcacc
     1201 cggctgtacg ccgccatcta cgcccttccg gagtcacggg tcgcggacgc cggccgactt
     1261 cgcggcgagg ctgccgaggt ccgcgatcgc ggggcgaccg aggatccggc cgggccggcc
     1321 gcacggggtc tcgcgtactg gccggaggtg gctcgcctct tgcgccagtc ctacgccgat
     1381 ctccatcggg ccctggccga gggcaactga cgccgcagag acgctgacgt gacggctccg
     1441 gggcagagtc ggccgtgcca cccgggcgcg acacgaggga ttcgagatgc cgctgttcgg
     1501 cgtggatgtc agccgtcacc agggcccgga catcaactgg ggcaaggttc gggcctccgg
     1561 catcgatttc atgctcgcgc gggccagcct ggcgacgacg cccgacgcca cctaccgaag
     1621 caatgtccga cgcgccaggc tggccgggat ccctgtcgtc ggcgcctacc acttcctgta
     1681 tccggcgaag gtcgtctcac ccgccgagca ggctcggctg ttcgtcgatc ggatcgggaa
     1741 cgccgacggc atcctgacga tgctcgacgt cgagaaggac cggaacccga agaccggcca
     1801 ggtcttcatt cccacgatca gcgacgtccg ggcgttcgcc agggagttcg cgcggttgac
     1861 gcacaaccac accctgatcg tgtacgcgcc cggctggtac tggaccgggc atatcggcaa
     1921 ccccccggcg tccgacctcg ggccgttgtg cgcgagtcgc tacgtcccgg tcaggcatga
     1981 ccacgagggt catccgatcg cgatgacccc gaaggaggcg ttcgcgaagg tcaccccgaa
     2041 gttctggaac ccgacgcatg gcggctggaa gaaggcaacg atcctccagt tcacgagcac
     2101 cggcaggatc ggcggctacg gcgggccggt cgacgtcgat gcgttcaacg gcacgctcca
     2161 gaagctgaag tcgctcacgg gtccggtcgg tcacgccgtg tcggcgctcg tcccgcctgc
     2221 ctcgaccggg cccgcgcccg accacgggaa gccgatcgtc gtcaagccca cgacgaagcg
     2281 gtcccatgtc gtcgtggccg gcgagacgct gtcggggatc gcggcccggc acggcttcaa
     2341 cgcgacggcc aagctgccga cgttccgggt catgctcaat gcgtttccgg agaacgcgaa
     2401 gttccgggcg aacccgcacg tgatccgtcc gggcgatcgc gttcgggtgc gctgaacgcg
     2461 gctcggcgca gtccggtcag ccttcgccgg cccgacggcc gacgacccac cacgccgccg
     2521 ggaacgtgac cgcggcaagg atcagcttga tcgcgtcgac gaggaggaac ggcgtgaggc
     2581 ccttggcgat cccggtcggg aggtccaggt gggcgacaac catgtaccac gggacgccga
     2641 tgacgtagat cacgacgttg ccgatcgcca tcgccccgag cgcgccgagg atccgtcgat
     2701 cccagccgag ctcggcgagc cgcccgacca gggagccggc aaccaggaat ccgacgatgt
     2761 agccgccgct cgcgccgagg atcacggaga ccccgccctt cgactcggcg aagaacggca
     2821 gaccgatcag accgatcaac acgtacagcc cgacgctcgc gagcccgcgc cggaatccga
     2881 gcgccccgcc aacgagcagc accccgaagg tctggccggt gatcgggacg tccgacccgg
     2941 gaatcgggat cgacacccgc gacgaggcga agaccagggt cgcgccgatc gcgatcagcg
     3001 cgatgtggcg cacgcggctg cccatccgct cgccgatccg gatcgggacg aggaagtcgc
     3061 cgatcgtgag gccccgttcc tcgacgggga gccggctcag ccgtgcactc gcgctgatca
     3121 tcgggtgacg gcgtcctccg ctcggctgga cgcccctccg cgagctcggt ggcccggtcg
     3181 cgtctgccgc cgcgagtcta ccatcgccct cccccgcccc ggctcattcg tgctgcaccg
     3241 cggccacgat cgcgaggcgg ctcgcgaggc gggccggata ccacgccgcg gccatcgaga
     3301 tcccgatccc gagcacgatc gcgaccccga tcgaggtcca cggcacctcg aggatgagcg
     3361 gcccaccacc ggcgagcagg acgaggatcg cacccgcgac gagcccggtg acgacgccca
     3421 ggatcgaccc cacgatcccg aggatcccgg cctcgacgac gaccgtgcgg cggacctggc
     3481 cgcgggtcat tccggccgcg cgcaggaccc cgagctcgcg gacgcgctcg aggacgctga
     3541 cggtgagcgt attgacgatg ccgagcgccg ccaccacgac ggcaacgagg gccagtgcat
     3601 cgaacaggcc gaagacctgg ctgagcgcgt cgtcgaccgc accggcgacc gtttcgagcg
     3661 gattcggctc gagggcggcg ttccgcgccg cggcttcaag ccgcggccgg gcctcggccg
     3721 cccggccggg cgtgaagcga acggcgagga catccgcgcc ctggacgccg aacaccgcgg
     3781 tcgcatcggg ccagccgacg aggacggcct caccgacgcc accgggcagg gtccgctcgg
     3841 tgatcccggc cacgcgcagg tcgacgacct gtccctcgcc gacggccagc gtcagcgtcg
     3901 agccgaccgt caggcccgtg cggttggcga tgctccgcgg caggaccgcg accccgccgg
     3961 catcgaggcc ctggagtgcg gccgtccgat cgccgtcgac gaaccgcagg cggccatcgg
     4021 ccagtagatc ggctccgtcg acggccgccg cgtccagccg gaccccgttg cgagcgacct
     4081 cgaagatcgc gaacggactg acccgggcga cgccatcgac cgccgccaga tcggcgatga
     4141 cgggctcgtc gagcgggatc ggccggatcg aggtcacgag ctcgtcgccg gggatgacct
     4201 cgtcgagcca ggccgaggcg gcgagacgcg actggtgggc gacgctgccg acggcgacga
     4261 ccatggccag cccgatcgcg agcgcaccga ccgtcagggt cgtccgcgac cggtcacgga
     4321 cgatcgcggc ccgcgccagg cgctcctcga cccggaggac gccccggaac ggcagcccgg
     4381 cgagccgacc caggacaccg agggcgaacg gagccgcgag gaccgcaccg aacaggacga
     4441 ggtagacgag cccggagcgg gcgagacccg cggtccccgc atcgcgcggc caggcgagca
     4501 ggccggcgat gccgacgacg acgaacaccc cgacgagcca gcgcagccgg gcccggcggg
     4561 cggcagaaga atcgatccga tcgcggagcg cctcgaccgg cgggatcgat ccggcccgcc
     4621 gggcgggctc gatcgccgcc gcgaccgtga cccccagccc gatcgcgatg accgccgcga
     4681 tcgacgcgag gctgggatcg acgcgctcga acggaatggc ggcgatcgaa cggaggtcgg
     4741 cggccatgag ctcggcgagg accaagccgg cgacgaggcc gacgagcgcc ccgaggagcc
     4801 cgaggacgac ggcctgggcc gcgacgaagg cgacgagctg gcgtcgggtc gccccggccg
     4861 cccggagcag gccgagctcg cggacccgtt ccgcgaccgt catcgagagc gtgttgaaga
     4921 tcaggaacgc gccgacgaac agggcgaccg ccgcgatcag ggccgtcgtc gagcggaagt
     4981 cggccgtcga gccgcgcaac gaggccgcca cgtcggccgg cgacgagatg acatacggct
     5041 ggctggtcag ggcgacatcg atcgcgctcg cgacctcggt cggggtcgcg ccttcgcccg
     5101 cgacgatgtc gacccggctg accgtgtcgt cggcgaggat ccgctgcatc gtcccgagcg
     5161 gcaggatgac cgtccgcccc gccgagccga cgatcgggcc gtcgccggcc aggatcccga
     5221 cgaccgtcag gtcgatcggc gcgccgtcac ttgccggcac cgtgatcgtg ccgccgacaa
     5281 cggtcccgtt ggccgtcgcg agggtctggg tgatcagggc ctggaagcca tcggggcccg
     5341 ccagcgacgc cccgctcgcc aggacgagat cgcggacggc cgcctcgggt ccgggatcga
     5401 tgccgagcac ggtgaccggc ggtggcgcgg cgttcggatc gtcgatcgcg gggacgagat
     5461 aggttcggcg ttggagcgcc ggcgcggcgc gggcgacgcc gggtgcggcc tcgatggcgg
     5521 ccaagctctc gggcgacagc cccgccgggc cgaacgactc gacgcgaagg tcggcccgcc
     5581 cgaccaggtc gcggaccgtc cggtcgatcg aggccgcgat tccggcatcg gtcgcaagcg
     5641 aggcgtacag gacagcgatc ccgagggcga tgccgacgat cgacaggctc gtccggccac
     5701 ggcgcgccgc gaggctccgg atcgcgagcg agctcaaccc gcccacggat cagagaccga
     5761 gctcggcgag gcggctcacg agcggaccgg tgtcgtgctc ggcgcgtcga ccgaggacga
     5821 tctcgtcgcg gatccggccg tcgctgatga ccaggacccg gtcggcgtag gcagcggcct
     5881 tcgagtcgtg ggtgacgagg acgaccgtct ggtgacgctc gacgcacgac cgccagagcg
     5941 cctcgaggat ctcggtcccg gtcgtgtagt cgaggttgcc ggtgggctcg tcggcgaaca
     6001 ggagggccgg ccgggtgacg agggcccggg cgatcgcgac ccgctgctgc tcgccggccg
     6061 acagctggtc gggcttgtgg cgctccttgc cggccagctc gacgagggcg atcacgtcgc
     6121 ggatccgctc ggcgatctcg cccttgcccg tgtccgctcc ggcgatcgtg aacggcaggg
     6181 cgacgttctc ggtgacgtcg aggagcggga tcaggttgaa cgactggaag acgaacccgg
     6241 tccggtcccg acggagcttc gtcgcggcat catcggagag ctggctgatc gtctcgccct
     6301 ggaggacgac ctcgcccgag gacgggcgat cgagcccgcc gagcagctgg agaagggtcg
     6361 acttgcccga tccggacggc cccatcaggg ccacgaactc gccttcgtcg acggtcagcg
     6421 agacgccgcg cagggcgtcg accgtggtgg cgccgaggcg atagcgcttg gcgagatcgt
     6481 gcgcctcgag gatcgcggcc atcgccggga gtgtagcccg cgccgaccgg cggtcgtcgg
     6541 ccggccgctc gggtcacggc gacttggtcg ggcgacctgc cgctgccgat gcgaatagcc
     6601 gaatgacatc ggagccgccg gccgagcgac ccccggccgg cattcggcta ttcgaacggg
     6661 gatggcgggt ggcgtggcga gcgccgaacc ggccggcacc gggtgacgaa ccgcggtcgc
     6721 cgctgtgccg cagcgcgatg cccgggtcag gcc
//
LOCUS       DATAIW010000493         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_202803, whole genome shotgun sequence.
ACCESSION   DATAIW010000493 DATAIW010000000
VERSION     DATAIW010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202803"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VHM48_14570"
     CDS             <1..550
                     /locus_tag="VHM48_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756690.1"
                     /translation="PFELAGLVERAVERLRPRLAGRTVELDVPDALPPVLVDEVFIDQ
                     VLTNLFENAIKYTPTPARIRVHAVASTTDPAAHPHGTVVLTVEDDGPGVPDDAVERLF
                     DRFFRVRRRGEPSRPGSGIGLTVVRGLVEAMGGRVGASRGSLGGLAIEVTLPSASPLP
                     DDVDRGAVGIGVAGDGGAGDGR"
     gene            547..1305
                     /locus_tag="VHM48_14575"
     CDS             547..1305
                     /locus_tag="VHM48_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756691.1"
                     /translation="MTTDHAPHVRILLVEDDDTTRSWLARDLTSRGYRVDEAADVATA
                     LNHWDAQRPDAILLDLGLPDRDGIAVVRRVRREAATPILIVSARGEETSKVEALEQGA
                     DDYLTKPFGTPELHARLRAVLRRAGGRAADVDGRVRNGEIVLDPLAHQVLVHDQPVAL
                     APREFELIRVLLDHPGRLVTRGRILRAVWGAAYDDESHYIHVYVSQLRRKLARADADG
                     CLADLFTAEPGVGYRVRTRASDAPAHDDQGAVKP"
BASE COUNT          210 a          591 c          550 g          221 t
ORIGIN      
        1 gcccttcgag ctggccgggc tcgtcgagcg ggccgtcgag cggctccgcc cgcggctggc
       61 cggacggacc gtcgagctcg acgtgcccga cgcgcttccg cccgtactcg tcgacgaggt
      121 cttcatcgac caggtcctga ccaacctgtt cgagaacgcc atcaagtaca cgccgacgcc
      181 ggcccggatc cgggtccatg ccgtggcctc gaccaccgat cccgcggctc atccccacgg
      241 tacggtcgtg ctgaccgtgg aggacgacgg cccgggcgtc ccggacgacg cggtcgaacg
      301 gctcttcgac cggttcttcc gcgtccgccg gcggggcgag ccgtcgcgcc cgggcagcgg
      361 gatcggcctg acggtcgtcc gcggcctggt cgaggcgatg ggcggtcggg tgggcgcgtc
      421 gcgcggctcg ctcggcgggc tcgcgatcga ggtgaccctg cccagcgcat cgcccctccc
      481 cgacgacgtc gatcgcggag ccgtcgggat cggcgtggcc ggagatggcg gcgccggcga
      541 cggccgatga ccaccgacca cgcgccccac gtccggatcc tgctcgtgga ggatgacgac
      601 acgacccgga gctggctcgc ccgcgacctg acgagccgcg gctaccgggt cgacgaggcc
      661 gccgacgtcg cgaccgcgct gaatcactgg gacgcgcagc ggccggacgc gatcctcctc
      721 gacctcggcc tgcccgaccg cgacgggatc gccgtcgtcc ggcgcgtccg ccgcgaggcc
      781 gcgacgccga tcctgatcgt ctcggcccgg ggcgaggaga catcgaaggt cgaggccctc
      841 gagcaggggg ctgacgatta cctcacgaaa ccgttcggaa caccggagct ccacgctcgc
      901 ctgcgcgccg tcctccgtcg cgccgggggc cgtgcggccg atgtcgacgg ccgggtccgg
      961 aatggcgaga tcgtcctcga tccgctcgcc caccaagtcc tcgttcacga ccagcccgtc
     1021 gctcttgccc cgcgcgagtt cgagctgatc cgcgtcctgc tcgaccatcc cggtcgcctg
     1081 gtgacgcgcg gccggatcct gcgcgccgtg tggggagctg cctatgacga cgagtcgcac
     1141 tacatccatg tctacgtcag ccagctccgc cggaagctcg cccgggcgga cgccgacggc
     1201 tgcctcgccg acctgttcac ggccgagccc ggcgtcggct atcgcgtcag gacacgggcg
     1261 tccgacgcgc cggcccacga tgatcagggc gcggtcaagc catagccgac cgcgcgcacg
     1321 acgcgcggtg ttgagcggtt cttgagcgcg aatcgatcgg ttcggcactt ggcctcttgg
     1381 tgctggcgag catcgtgagg acgtgagcga caccccacgt cccgaccatg ccgaccagcc
     1441 tgcgcggagg gcaatcgacg gtgaccggat ccggtccgga ctcgaccgcg agcgacggat
     1501 ggtcgccgag gcgatcctcc tcgtctcgag tggcggctcc cggcgggtga ccgtcgccgg
     1561 cctccactac cc
//
LOCUS       DATAIW010000494         7370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_293235, whole genome shotgun sequence.
ACCESSION   DATAIW010000494 DATAIW010000000
VERSION     DATAIW010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293235"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VHM48_14580"
     CDS             <1..702
                     /locus_tag="VHM48_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2756692.1"
                     /translation="AGVTHSPHETRAAGILRPVVFGGNDGLVSNLALVMGVAGAAQAP
                     GIIVLAGIAGLLAGAFSMAVGEYISVQSQRELLDYQVAFQRRQLKETPDQEYDILVDI
                     YLGRGLNREEAKHFADRMFADPQRALETLLREEVGLDPRAIGSPISAAVGSFIAFTLG
                     ALVPVVPYLIGSGPVAFWASLVASLVALFFLGLGVSQFTHRHPLRSGARQLLLGLVAA
                     VVTYGVGTALGAAGI"
     gene            complement(797..1999)
                     /locus_tag="VHM48_14585"
     CDS             complement(797..1999)
                     /locus_tag="VHM48_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2756693.1"
                     /translation="MTNPSMSDAMRASVAPFLGFLTGPIMATLTRPEVANFAFGNPNE
                     MPLPGYVDALRTHLEPQSTDWFAYKLSEPKSQAAVARTLTVRTGLDWDPADVAMTNGG
                     FAALAVAFRALLEPGDEVVFLSPPWFFYEILIIAAGGVPVRVPLAPPAFDLDPATIAA
                     AITPRTRAVLLNSPHNPSGRVYPLADLERLATTLRDASARIGRTITLVSDEPYNRILF
                     DGRVFHSPAEVYPDTVITYSYGKTLLAPGMRIGYLTVPPTMDGREDFRERVLIAQIAT
                     GYSFPNALLQHAIEDIERLSIDIAALQRRRDRVVGALDGMGYQPTTPEGTFYVMARAP
                     IDDDVAFCELLAEENVAVLPGTVVEVPGWFRVSLTANDQMVEASLAGFERARARASSG
                     AEVAGVPA"
     gene            complement(2200..4266)
                     /locus_tag="VHM48_14590"
     CDS             complement(2200..4266)
                     /locus_tag="VHM48_14590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX2756694.1"
                     /translation="MLSAPPLEDHLRTMLGPEVTFRDGQREAIEAVSGDGARALVVQR
                     TGWGKSLVYWIATRVRRDTGHGPTLIVSPLLSLMRNQIAMAARLGLEAVTINSGNVGE
                     WREIEGRLERNEIDVLLISPERLANEGFTSRVLPAIERTIGLLVIDEAHCISDWGHDF
                     RPDYQRISRLLPLLGPTVPVLATTATANDRVVEDVAAQLGAHSAIIRGPLARDTLRLD
                     AIPLADQAERLAWLAENIPRLPGSGIIYCLTVADTERVATWLRGRGIDARAYNGPMAQ
                     PDREALEDALIANEMKALVATVALGMGFDKPDLGFVVHYQRPGSAIAYYQQVGRAGRA
                     VERAYGILLSGREDDEIADYFLRTAFPPADRMRELLGAFDGVESASIGRFQRAVNLTR
                     GQLDQALKQLELDGAVTKSGGRWSRTAEPWAPDEERIARVTATRRAELEQMQAYVTTD
                     GCRMAYLTGLLDDPATAPCGRCANDVGKGMSHVVDGEVVRAAIDYLRRSLRPIEPRKR
                     WLEADGASTGTTIADPNAPGIALCVAGDAGWGREIVRARGGVGRFPDGLVVAATRAIR
                     DQWRPDVPPEWVTSVPSRTAAGLVRGFAEALAAELGLPYVDCLTSSADRPPQAAMQNS
                     VLQLENARAKLGIDRAAVRPGAVLLVDDLVDSRWTLTVAGSLLRASGSGTVLPFTLAQ
                     ASARDS"
     gene            4409..5218
                     /locus_tag="VHM48_14595"
     CDS             4409..5218
                     /locus_tag="VHM48_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2756695.1"
                     /translation="MSTTAATSGLASVNGIELAWQSFGEGGLAPLVLLHGGFGSVEMF
                     GPNVAALAAARRVIGVDLQSHGRSPTANRPMRFETMADDIAALIGSLGHRQADVLGFS
                     LGGGVALRTAIQHPALVRRLILVSTPFKRAGWHPEMVAGMDSMGPGIAPMMRQTPLGE
                     AYAAIAPRVDDWPVLVTQLTDLLKVDYDWTDEARGLQPATMIVAGDADGLSPRHAVEF
                     FELLGGGLLDASWDRSGMTKHRLAILPGATHYDINVVPELARTAARFLDER"
     gene            complement(5329..5583)
                     /locus_tag="VHM48_14600"
     CDS             complement(5329..5583)
                     /locus_tag="VHM48_14600"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2756696.1"
                     /translation="MLAPAAQRQLRRLPPGEAARMRGPILALGIEPRPPGSMKLSGTE
                     FWRLRVGDLRIVYLIDDGESLVVVLRVARRSESTYRRIEP"
     gene            complement(5604..5861)
                     /locus_tag="VHM48_14605"
     CDS             complement(5604..5861)
                     /locus_tag="VHM48_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756697.1"
                     /translation="MKVTIDLEADVYRAVKVEAARTDRAIRDIVEEALGAWLESSEAD
                     EDRASAAAALDEYRRDGGQAAESFFGTLAAETRATYGSGEG"
     gene            6079..6333
                     /locus_tag="VHM48_14610"
     CDS             6079..6333
                     /locus_tag="VHM48_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756698.1"
                     /translation="MEQRTKRTYRLTGRAQARVREMATEYGIGGSQDAVVELAVDRLY
                     REVEAAAEAQRWVAAATDPVFTAEAADIDREFAEIETWPS"
     gene            6333..6752
                     /locus_tag="VHM48_14615"
     CDS             6333..6752
                     /locus_tag="VHM48_14615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX2756699.1"
                     /translation="MAAVERWSIVILDLDPVVGHEQAGRRRALVVSYEPFHRSGMATV
                     CPITSRAPKYPGEIAVPAGHAGQTLDGAILVHQIRTIDVRRLTAAIIGGAAQTVTDPA
                     IRRDVRRALAHHLGLDVPTAADGAAPALVPATDRVTD"
     gene            complement(6756..>7370)
                     /locus_tag="VHM48_14620"
     CDS             complement(6756..>7370)
                     /locus_tag="VHM48_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756700.1"
                     /translation="IYDPATGRFALVGSLPKIDRAALEKQATKGANPMPDDDGEANGT
                     GILVALADGGAVLIARTNYWKHVADMTRSFRFDASSGKWSEVGQTYAFVGEPTAVPLW
                     TPGVPALVGARVATLRDGQVLVAGGQGPNLFGETGYNSTPTTAVARLYDPATNAWLAL
                     PALPEPRSGGATVVLSDGSVLVVGGSVDTTDGSLNVKTAIRFVP"
BASE COUNT         1057 a         2629 c         2593 g         1091 t
ORIGIN      
        1 gcgggcgtga cccactcgcc gcacgagaca cgcgccgccg gaatcctccg gccggtggtc
       61 ttcggcggca acgacgggct cgtcagcaac ctcgccctcg tcatgggcgt cgccggcgcc
      121 gcccaggcac ccgggatcat cgtcctcgcg gggatcgcgg gtctgctcgc cggcgcattc
      181 agcatggccg tcggcgagta catctcggtc cagtcgcagc gcgagctcct cgactaccag
      241 gtcgccttcc agcgccgcca gctcaaggag acgcccgacc aggagtacga catcctcgtc
      301 gacatctacc ttggccgggg cctgaaccgg gaagaggcca agcacttcgc cgaccggatg
      361 ttcgccgacc cccagcgggc gctcgagacg ctcctccgcg aggaggtcgg gctcgacccg
      421 cgggcgatcg gctcgccgat cagcgcggcg gtcggctcct tcatcgcgtt cacgctcggg
      481 gctctcgtgc cggtcgtgcc ctacctcatc gggtccggac cggtcgcgtt ctgggcgagc
      541 ctggtcgcga gcctcgtggc gctgttcttc ctgggcctcg gcgtcagcca gttcacccac
      601 cgccacccgc tccggtcggg ggcgcggcag ctgctcctcg gcctggttgc cgcggtcgtc
      661 acctacggcg tcgggaccgc gctcggcgcc gccgggatct gaccgagatc ggacggggga
      721 ggtgggatgg acgcgggttc cgacgactga cgacatcgga tcgtcccagg cagggattcc
      781 ggccgggctg gagacctcag gcgggaacgc cggccacttc ggctccgctg cttgctcgcg
      841 ctcgggcccg ctcgaagccc gcgaggctcg cctcgaccat ctggtcgttc gcggtcaacg
      901 agacgcggaa ccagcccggg acctcgacga ccgtcccggg caggaccgcg acgttctcct
      961 cggcgaggag ctcgcagaag gcgacgtcgt cgtcgatcgg cgcccgggcc atcacataga
     1021 acgtgccctc cggggtcgtc ggctgatagc ccatcccatc gagcgcgccg accacgcgat
     1081 cgcgccgccg ctggagcgcc gcgatgtcga tcgacaaccg ctcgatgtcc tcgatcgcgt
     1141 gctggaggag ggcgttcggg aacgaatagc cggtcgcgat ctgggcgatc aggacgcgct
     1201 cgcggaagtc ctcccggccg tccatcgtcg gcggcaccgt gaggtagccg atccgcatcc
     1261 cgggcgcgag gagcgtcttg ccgtacgagt atgtgatcac cgtgtccggg tagacctcgg
     1321 ccgggctatg gaacacgcgg ccatcgaaca ggatccggtt gtatggctcg tcggagacca
     1381 acgtgatcgt ccggccgatc cgggccgacg cgtcgcgcag cgtcgtcgcc aatcgctcca
     1441 ggtcggcgag cgggtagacg cggccgctcg ggttgtgcgg cgagttcagg aggacggccc
     1501 gggtccgggg cgtgatcgcc gccgcgatcg tcgccgggtc gaggtcgaac gccggcggcg
     1561 ccagggggac acggaccggc acgccaccgg cggcgatgat caggatctcg tagaagaacc
     1621 acggcggcga gaggaagacg acctcgtcac cgggctcgag gagcgcccgg aaggcgacgg
     1681 ccagggcggc aaacccgccg ttggtcatcg cgacgtcggc cggatcccag tcgaggccgg
     1741 ttcgcacggt cagcgtccgg gcgaccgcgg cctgcgactt cggctcggag agcttgtacg
     1801 cgaaccagtc ggtcgattgc ggctcgaggt gcgtccggag ggcgtcgacg tagcccggca
     1861 gtggcatctc attggggtta ccgaacgcga aattggcgac ctcgggacgg gtgagcgtgg
     1921 ccatgatcgg cccggtcagg aagccgagga acggcgcgac cgacgcccgc atggcgtcgc
     1981 tcatggatgg gttggtcatc gtggaatccc tcatggagcc gtgccggata cggacgctgt
     2041 cgccgagcgg ctgtgcgcca cgtggctcgt ctcgtgggcg gcagcgtact gccagtccgg
     2101 aaggtgatgt cgaaccgacg acgatgagac ccgcccagcg ggccgattca cgggtcgttg
     2161 cgcgcatccg gaacgagttc gcctgcggcc tggtccctct caggagtcgc gggcactcgc
     2221 ctgcgccagc gtgaacggca ggaccgtccc gctcccggag gcgcggagca gggaccccgc
     2281 caccgtcaag gtccagcgac tgtcgacgag atcgtcgacg aggaggacgg cgcccgggcg
     2341 gactgcggcc cgatcgatcc cgagcttggc ccgggcgttc tcgagctgga ggacgctgtt
     2401 ctgcatcgcc gcctgcgggg gacggtcggc ggaggatgtc aggcaatcga catagggcag
     2461 gccgagctcg gcggcgagcg cttctgcgaa cccacggacc agcccagcgg cggtccgtga
     2521 cgggaccgag gtgacccatt ccggcggcac gtccggtcgc cactggtcgc ggatggcgcg
     2581 ggtcgcggcc acgaccagcc cgtcagggaa tcgcccgacg ccgccgcggg cccggacgat
     2641 ctcgcgtccc cagccggcgt caccggcgac gcacagggcg atcccgggag cattcgggtc
     2701 ggcgatcgtg gtgccggtcg aggctccgtc agcctcgagc cagcgcttcc gcggctcgat
     2761 gggtcggagg cttcggcgga ggtaatcgat cgccgcccgg accacctcgc cgtcgaccac
     2821 gtgcgacatc cccttcccga cgtcgttggc gcaacgaccg cagggggccg tcgctggatc
     2881 gtccagaagg ccggtgagat acgccatccg gcagccgtcc gtggtcacgt aggcctgcat
     2941 ctgctccagc tccgcgcggc gcgtcgccgt gacccgggcg atccgctcct cgtcaggcgc
     3001 ccacggctca gccgtccgcg accagcggcc gccgctcttc gtgaccgccc cgtcgagctc
     3061 gagctgcttg agggcctggt cgagctggcc gcgggtgagg ttgacggccc gctggaaccg
     3121 cccgatcgag gccgactcga ccccgtcgaa cgcccccagc agctcacgca tccgatcggc
     3181 cggtgggaac gcggtccgca ggaagtagtc ggcgatctcg tcgtcctcgc gacccgagag
     3241 gaggatcccg tacgcccgct cgactgcccg cccggcccgg ccgacctgct ggtagtaggc
     3301 gatcgccgag cccggccgct ggtaatggac gacgaacccg aggtccggct tgtcgaagcc
     3361 catcccgagc gcgaccgtcg cgacgagcgc cttcatctcg ttcgcgatca gggcgtcctc
     3421 gagggcctcg cggtccggct gggccatcgg gccgttgtaa gcgcgggcgt cgatgccgcg
     3481 gccacggagc caggtcgcca cgcgctccgt gtcggcgacg gtcaggcagt agatgatccc
     3541 gctgccgggc agccgcggga tgttctccgc gagccacgcc agccgctccg cctggtcggc
     3601 gagcgggatg gcgtcgagcc gcagggtgtc gcgggcgagc ggcccgcgga tgatggccga
     3661 gtgcgccccg agctgggccg cgacatcctc gacgacccgg tcgttcgcgg tcgccgtggt
     3721 ggcgagcacc gggacggtgg gcccgaggag cggcaatagc cggctgatcc gctggtagtc
     3781 cggccggaag tcgtggcccc agtcggagat gcagtgggcc tcgtcgatga cgagcaggcc
     3841 gatcgtccgc tcgattgccg gcaggacccg gctggtgaag ccctcgttgg cgagccgctc
     3901 cggcgagatc aggaggacgt cgatctcgtt ccgttccaac cggccctcga tctcgcgcca
     3961 ctcacccacg ttgccggagt tgatcgtcac cgcctccagg ccgagccgcg ccgccatcgc
     4021 gatctggttc cgcatcagcg acaggagcgg gctgacgatc agggtcggtc cgtggcccgt
     4081 gtcgcgccgg acgcgggtgg cgatccagta cacgaggctc ttgccccagc cagtccgctg
     4141 gaccacgagc gcccgggcac cgtcgccgct caccgcctcg atcgcctcgc gctggccgtc
     4201 gcggaacgtg acctccgggc cgagcatcgt ccggaggtgg tcctcgagcg gcggcgcgct
     4261 cagcaagccg tcacgtggtt cctcatggcg tcatgatcgc agggcaggct catctgacga
     4321 tcttcgatga ggttcgaggc cgggccgctt ccatgggcag ccggcgcgaa cgggcgcccg
     4381 aacgaggcca atgacgaagg agcccatcgt gtccacaacc gcagccacct ccggcctcgc
     4441 ctccgtcaac ggcatcgaac tcgcctggca gtcgttcggc gagggcgggc tcgcgcccct
     4501 ggtcctcctc catggcggct tcggctcggt cgagatgttc ggcccgaacg tcgccgcgct
     4561 tgccgccgct cggcgggtga tcggtgtcga tctccagagc cacggccgct cccccaccgc
     4621 caatcgcccg atgcgcttcg agacgatggc cgacgacatc gccgccctga tcggatccct
     4681 cggccaccgc caggcggacg tcctgggctt ctcgctcggc ggcggcgtcg ccctccgaac
     4741 cgccattcag cacccggcgc tcgtccggcg cctgatcctc gtgtcgaccc cgttcaagcg
     4801 ggccggctgg cacccggaga tggtggccgg gatggactcg atggggcccg ggatcgcgcc
     4861 gatgatgcgc cagacgccgc tcggcgaggc gtatgcagcc atcgctccac gggtcgacga
     4921 ctggccggtc ctcgtcaccc agctgaccga cctcctcaag gtcgactacg actggacaga
     4981 cgaggcgcgc ggtctccagc ccgcgacgat gatcgttgcc ggcgacgccg acggcctgtc
     5041 gccgcgccat gccgtcgagt tcttcgagct cctcggcggc ggcctgctgg atgcgagctg
     5101 ggaccgctcg gggatgacca agcaccgctt ggcgatcctg cccggcgcga cccactacga
     5161 catcaacgtg gtccccgagc tggcgcggac ggccgcccga ttcctcgacg aaaggtgacc
     5221 gccctggggc tgatcggcga taccgggcgc cgaaccgggc tgatcgtcga cgacggtcga
     5281 cgggacggac tccggcctgc tccagcgggc tgacgcggcc cccgctcgtc agggttcgat
     5341 ccgccggtag gtgctctccg atcgccgggc gacccgcagg acgacgacca gggactcgcc
     5401 gtcgtcgatc aggtagacga tccggaggtc gcccaccctg aggcgccaga actccgttcc
     5461 gctgagcttc atcgagcccg gcggtcgtgg ttcgatcccg agcgcaagga tcgggccgcg
     5521 cattcgggcc gcttcgcccg gaggcaggcg ccgcagctgg cgctgggcgg ccggggcgag
     5581 cacgactcga ggcctgctcc cgctcacccc tcgcccgagc cgtacgtcgc tcgggtctct
     5641 gcggcgagcg ttccgaagaa cgactcggcg gcctggccgc catcgcgtcg atactcatcg
     5701 agcgccgcgg cggcggacgc gcggtcctcg tcggcctcgc tggactcgag ccaggccccg
     5761 agcgcttcct cgacgatgtc gcggattgcg cgatccgtgc gagccgcctc gaccttcacg
     5821 gcgcggtaga catcggcctc cagatcgatg gtcactttca tgcgaaccat gataccagca
     5881 acccagcatg ctggtacgcc acgggactga aagaacgcct cctggggcag aagctgtcgg
     5941 cgccgctggg catcgtcctg gtcgctgccg ggagttcgct tctccttgcc acgctcgtcg
     6001 cggtctgacg ctccctggca gcgtcgacgc tggacacgtc gaattgacac gtcgttcgcg
     6061 cacgtccaga cttggcttat ggagcagcgg acgaaacgga cctatcgcct gaccggacgc
     6121 gcccaggcgc gtgtccgcga gatggccacg gagtacggca tcggcggctc gcaggacgcc
     6181 gtcgtggagc tcgccgtcga tcggctctat cgcgaggtcg aggccgcggc cgaggcgcaa
     6241 cgttgggttg cggcagccac ggacccggtg ttcacggcgg aggccgccga catcgatcgg
     6301 gagttcgccg agatcgagac ctggccgagc tgatggcggc cgtcgagcgt tggtcgatcg
     6361 tgatcctcga tctcgatccg gtcgtcgggc acgagcaggc gggccgacga cgagcgctgg
     6421 tcgtctcgta tgagccgttc catcgctccg gcatggcgac ggtctgcccg atcaccagtc
     6481 gggcgccgaa gtaccccggc gagatcgccg ttccggccgg ccacgccggc cagaccctcg
     6541 acggcgcgat cctcgtccac cagatccgca cgatcgacgt gcgccgactg accgccgcga
     6601 tcatcggcgg cgccgcacag acggtcaccg atcccgccat ccgtcgtgac gtccgccgtg
     6661 cgctcgccca ccatctgggc ctcgatgttc cgacggccgc agacggcgcg gccccggcgc
     6721 tggtgccggc gaccgaccga gtgaccgact gaccgtcagg gaacgaaccg gatcgcggtc
     6781 ttcacgttga gcgatccgtc ggtcgtatcg acggatccgc cgaccacgag caccgaccca
     6841 tcggacagga cgacggtcgc gccgccggat cgtggctcgg gcaaggccgg gagcgccaac
     6901 caggcgttcg tcgccgggtc gtagagcctt gcgaccgccg tggtcggggt cgagttgtag
     6961 ccggtctcac cgaacaggtt ggggccctgg cctcccgcga ccaggacctg cccgtcgcgg
     7021 agcgtggcga cccgggcacc gacgagcgcg gggaccccgg gtgtccacag gggaaccgcg
     7081 gtcggctcgc cgacgaacgc gtacgtctgg ccgacctcgg accacttccc gctcgaagcg
     7141 tcaaaccgga aggaccgggt catgtcggcg acatgcttcc agtagttggt ccgggcgatc
     7201 aggaccgcgc cgccgtccgc cagggcaacc aggatccccg ttccattcgc ttccccgtcg
     7261 tcgtccggca tcgggttggc acccttggtc gcctgcttct cgagcgccgc ccgatcgatc
     7321 ttcggcaacg agccgaccag cgcgaaccgc ccggtggcgg ggtcgtagat
//
LOCUS       DATAIW010000495         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_233179, whole genome shotgun sequence.
ACCESSION   DATAIW010000495 DATAIW010000000
VERSION     DATAIW010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233179"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..455)
                     /locus_tag="VHM48_14625"
     CDS             complement(54..455)
                     /locus_tag="VHM48_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756701.1"
                     /translation="MRRFLISLALAGSMLGIAAPVALGHECYIVNRSDQGNAAVLHSG
                     RWVQLTLADIFGFINTAVGGPALSPSQITWAVDTAVAQGLPRDGWVVRSDKTIGEGSK
                     NPNLADGKGLDHLADVYGGQIVGIYFQALGH"
     gene            658..>2097
                     /locus_tag="VHM48_14630"
     CDS             658..>2097
                     /locus_tag="VHM48_14630"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX2756702.1"
                     /translation="MRPGLNILEPELIGRIVDEAKRVLAEHGFEIRGAEMRRRLIEAG
                     CPQREDGRILFPRDIVEAAIRTAPASFQLFDRAGIPSADIGEDRVHFIPGSSGLKVLD
                     HRTGEARQATSLDFAEYIRLGDGLANIPYLATAFSTGDLEPQVSDAWRLYLCLTNSTK
                     PVVSGAFTEHGVPRMAEMMELFRDGADDLRARPMSIFTITATGNFRYSEDSCQNLIDC
                     VEAGIPVEIVPVTLMGLIAPVTLVGATVFHTIDVLAGITMAQVVRPGAPVLFGGAPAT
                     FHMKIASSPMAAIEALQLDVAYVAVAKAIGLPTQSYMALSDAKTLDAQAGAETFGSAL
                     LAALAGVNSVSGPGMLDFLLVFSLPKLVFDDAMCGQVLHFVREIRELNDLPVRELIDQ
                     LMADQHLIMAPHTTEHWPRELYLPSTLIDRDNREAWTRLGAKEIDERAIDEVNRRLGA
                     YRQLETDPLLDAELQRIIRSGMVDLRVTSA"
BASE COUNT          320 a          731 c          717 g          329 t
ORIGIN      
        1 gatcgccggg ctcgagggcc cggcgatcgt cactgcgcga aggaagtcga gcctcagtgg
       61 ccgagcgcct ggaagtagat cccgacgatc tggccgccgt agacgtccgc cagatggtcg
      121 agccccttgc cgtcggcaag gttcgggttc ttcgagccct cgccgatggt cttgtcggac
      181 cggacgaccc acccgtcgcg cggcaggccc tgggcgacgg ccgtgtcgac cgcccacgtg
      241 atctggctcg ggctgagcgc cgggccgccg acggccgtgt tgatgaaccc gaagatgtcg
      301 gccagcgtca gctgcaccca acgaccggaa tgcaggacgg ccgcgttgcc ctggtccgag
      361 cggttgacga tgtagcactc gtgcccgaga gcgaccgggg ctgcgatgcc gagcatggat
      421 ccggcgagtg cgaggctgat caggaatcgg cgcatcgagt ctccttctgt catggtcccc
      481 ggcgaaacgt caaggtaccg ttcggggcgg agcggggcaa tccggtaagg tgcggattcc
      541 gacgcctgcg gtgtctggac gcaccgaact gcgaaggcca caacgatccg cggcgtgcaa
      601 ctggccgggt tttcggcgtg tcagccccga ccatcggtcg ggcatgatgg agcggcgatg
      661 cgacccggcc tgaacatcct cgagcccgag ctgatcgggc ggatcgtcga cgaggcgaag
      721 cgcgtcctcg ccgagcacgg cttcgagatc cgcggagcgg agatgcgacg gcgcctgatc
      781 gaggccggtt gcccgcagcg cgaggacggc cggatcctct tcccgcgcga catcgtcgag
      841 gcggcgatcc ggaccgcacc cgcgtcgttc cagctgttcg accgggccgg gattccgagc
      901 gccgacatcg gcgaggaccg ggtccacttc atcccgggct cgagcgggct caaggtcctc
      961 gaccaccgga ccggcgaggc gcgccaggcg acgtccctcg acttcgccga gtacatccgg
     1021 ctcggcgacg gcctcgcgaa catcccgtat ctcgcgaccg ccttctcgac cggcgacctc
     1081 gagccccagg tctcggacgc gtggcggctc tacctgtgcc tgacgaactc gaccaagccg
     1141 gtcgtgtcgg gcgcgttcac cgagcacggc gtgccgcgga tggccgagat gatggagctg
     1201 ttccgggacg gcgccgacga cctgcgggcc cgcccgatgt cgatcttcac gatcaccgcg
     1261 accggcaact tccgctattc ggaggactcc tgccagaacc tgatcgactg cgtcgaggcc
     1321 gggatcccgg tcgagatcgt gccggtcacc ctcatgggcc tgatcgcccc cgtgaccctc
     1381 gtcggtgcga cggtcttcca cacgatcgac gtcctcgccg ggatcacgat ggcccaggtc
     1441 gtccgacccg gggcgccggt cctgttcggc ggagctccgg cgacgttcca catgaagatc
     1501 gcctcgtcgc cgatggccgc gatcgaggcg ctccagctcg acgtggcgta cgtcgcggtc
     1561 gcgaaggcca tcgggctgcc gacccagtcg tacatggccc tgtccgacgc gaagacgctc
     1621 gacgcccagg ccggcgcgga gacgttcggg tcggcgctgc ttgccgcgct cgccggggtg
     1681 aacagcgtgt ccggcccggg gatgctcgac ttcctgctcg tgttctcgct cccgaagctc
     1741 gtcttcgacg acgcgatgtg cggccaggtc ctccatttcg tgcgcgagat ccgcgagctc
     1801 aacgacctgc cggtccgcga gctcatcgac cagctcatgg ctgaccagca cttgatcatg
     1861 gcaccgcaca cgaccgagca ctggccgcgc gagctgtacc ttccgtcgac cctgatcgac
     1921 cgcgataacc gtgaggcctg gacgcggttg ggcgcgaagg agatcgacga gcgggcgatc
     1981 gacgaggtga accgccgtct cggcgcctac cgccagctgg agaccgaccc gctcctcgac
     2041 gccgagctgc agcggatcat ccggtccggg atggtcgatc tccgcgtcac gtcggcc
//
LOCUS       DATAIW010000496        10763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_203546, whole genome shotgun sequence.
ACCESSION   DATAIW010000496 DATAIW010000000
VERSION     DATAIW010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203546"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VHM48_14635"
     CDS             <1..544
                     /locus_tag="VHM48_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2756703.1"
                     /translation="GRLELLSVDGREVLLDGAHNPAGAAALAAALDELRPFLGAGRVT
                     LLTASMADKDVGGVVDALAGSTALADARIVATAVAVPRALPADELARAWSRRSGEPAG
                     AYRTVAAEPDVDAALDRILAGPTGPVVVAGSLYLVGAVRARLVDDPLLHDPTPDDASP
                     PDARDRSRPSAADPGDNPSR"
     gene            541..1548
                     /gene="folP"
                     /locus_tag="VHM48_14640"
     CDS             541..1548
                     /gene="folP"
                     /locus_tag="VHM48_14640"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2756704.1"
                     /translation="MTDRSPTRPDVVAARPELGGGATTPSFGLPADAPTGIPGPAARL
                     QIGPAPFDWGTRTFVMGILNVTPDSFSGDGLLASTPGATDPDPVAAAVAIGRRMVDEG
                     ADLLDVGGESTRPGHEVVADDDEIARVVPIVRALRAALPDTPLSIDTTKVAVAAAALD
                     AGANLVNDVWGVGQDDALLRLAAAVGAPIVLMHNRAEARYMNLMAEVVADLQRAIERA
                     IRCGIAPESIVIDPGFGFGKTPAHNLVLLRDLAILRILGRPILLGTSRKSTLGKVLDL
                     PADQRLEATLATTALGVAAGVDIVRVHDVRANVRAARMADAIVRRPGPSAAHPTEGDQ
                     P"
     gene            1560..1913
                     /gene="folB"
                     /locus_tag="VHM48_14645"
     CDS             1560..1913
                     /gene="folB"
                     /locus_tag="VHM48_14645"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2756705.1"
                     /translation="MLHNIQLQGRHGYYDHELAAPQPFEVDVELMLNLQPAGIDDDLA
                     KSVDYARVHEVVRQIVESTSYRLLEALAEAISHEILIEFPVVEVGVRVRKPAVQLGGP
                     IDYAGVEIWRRRSPG"
     gene            complement(1966..3495)
                     /locus_tag="VHM48_14650"
     CDS             complement(1966..3495)
                     /locus_tag="VHM48_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2756706.1"
                     /translation="MTDRFSDPTDETQPHDAAAWRPIDRAAAADMPAAQATPAAFERA
                     AATADAPAGTTSPNPSAVRDGWPAVADPPRDDRAVDPLIGPASFSPTPEPRTDWTRTS
                     WSDREPTPEHWFETATPVPVRPIVTTPRRGAGTGTVLATALVAAVLASGGTIAALTAT
                     GALDRTPVAATGSQNPNSTTIKQPVTLDENSAIIDVAAKAGPSVVRIYTKGVDPNSAT
                     QQEQEGVGSGIIFDSAGWILTNRHVVAGTTDLTVQLKDGTKYDATVYGIDTLTDLAII
                     KVDATGLPAATIGDSDGLKVGELVVAIGSPLGTFDNSVTSGIVSALGRDITTDGGHLR
                     NLVQTDAAINPGNSGGPLLDATGAVIGVNTAIAKDSTGIGFSIPIDIARPIMRQALAG
                     EALARPYIGIQYVSIDPQIYKHLTLPATAGAYVQLLDENGDPTGAEAVRPNSPAANAG
                     IKTGDIVIAINGQTIDALHPLDAVLTQFAPGDKITLEVIRGTEHKTVAVTLGVRPKDL
                     S"
     gene            complement(3589..5337)
                     /locus_tag="VHM48_14655"
     CDS             complement(3589..5337)
                     /locus_tag="VHM48_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2756707.1"
                     /translation="MVDLDSVVAADPDADADADADADPTTVGRPGRRSRFHRPRLIPR
                     MIRRAGPKSLQARLTLGFASVVALSLLLVTVFVLNRLDDEFRTQQIADLQVRAQLVSE
                     YVDTLNKDSAQGLPVVFADNHVNPDVADELGSNAQARFIADRLAEADVDIVLGLPSAG
                     GADAGTTVAAIDGSFHASVRSPSRPGLTKETLRAAPLIRVSTESDFPYVIEVRLSNPY
                     TFRQSAIDNVTTVTAAVGALALGIAVLVAAALALRVTTPLRRMTEASRALAEGELGRR
                     IQRADIRSGSSEIAALAAQFNAMADQLEASVAMIRRDRDRSRDFLADVSHELRTPIAA
                     LLTFNELLTERTGDDPAARAEFLNSSRIQLERLDWLAQNLLELSKLDSGLVLLDLRPD
                     DLRAAVESAVEQAGPAARRRGVALRLEPATAPIRLRHDPQRIGQVVTNLVGNAIKFTE
                     PGGSVAVSVAAVADGGARIEVADTGVGIEPAELPRIFERFYRGSRANEARGSGSGLGL
                     AIVRSIVDMHRGTVEVVSRLGAGTTFTVNLPADPRREDDLGTTASTTLEPPTDLPKMV
                     DSSPTGAPHLNRETSG"
     gene            complement(5411..6109)
                     /locus_tag="VHM48_14660"
     CDS             complement(5411..6109)
                     /locus_tag="VHM48_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756708.1"
                     /translation="MSATILIVEDEFAVARGIQYALQQEGYQVAVAKSGEEGLDFATN
                     QAPDLVVLDVRLPGIDGFEVLRRLRASGSKAPVLVLTARDDEVDKVIGLELGADDYLT
                     KPFGLRELMSRIKALLRRAYGDLSDAAGGRVIRHGDLVIDLERRRVQRGDRRISLTPT
                     EFEILRHLASRPGRVYTRGELLELLRDYEALDQDEKTINVHVSHLRDKIEDDPAVPAF
                     VMTVRGVGYAFAER"
     gene            6262..6969
                     /locus_tag="VHM48_14665"
     CDS             6262..6969
                     /locus_tag="VHM48_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX2756709.1"
                     /translation="MSTATTTRLNLAAEPRELVGKKVATLRRVGRLPAVVYGHGVSSE
                     NVSLDAHDFELLRRKAGQNELIDLSVDGKKARPVLVHSVQVHAVNRRPLHVDLFLVRM
                     TEELTVDVPLVAIGTSNAVENLNGTLMHQLESVRIKALPDHLPQSIEYSIDSLVDFDT
                     VVKVSDLAIPSDVTLLTDADEVVAKVLASRLQAEAAELAAETAETVAEAAETAEGGEA
                     AEGATEAATDGGDSAES"
     gene            complement(7148..7579)
                     /locus_tag="VHM48_14670"
     CDS             complement(7148..7579)
                     /locus_tag="VHM48_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2756710.1"
                     /translation="MTTADPPGIGVLGFDHVQLAMPVRGEAEARRFYGTVLGLREVAK
                     PDTLRGRGGCWFVGPGATAIHLGVDDRFIAAKKAHPCLIVTDLDAARRALVAAGAALT
                     EDDAGTGLIRCYTADPFGNRIELVDASDAGFTDRTLTVDDR"
     gene            complement(7576..8397)
                     /locus_tag="VHM48_14675"
     CDS             complement(7576..8397)
                     /locus_tag="VHM48_14675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2756711.1"
                     /translation="MTEQAERYDRIAEGYGRWWAPIIAPAAVTILDEVADAIEAGATS
                     VLDVGTGTGTLAIAILRRWPRVRVTAIDGSGGMIAMATAESDRSLTAAQRDRLELRVA
                     FADDLGLGDGSIDVAVSSFVLQLVPNRFRALREAHRVLRPGGVFGWVSWLTHETIWAP
                     DVDFDDALVEVGEEPRGWPDDHDDLRDAAAAAAQMRRAGFAAVRATAGELVHPFDIDG
                     YVGFMAEFDEEDLVRGLEPDVRARFDAALRRRLARHRPDQLAMRQLTVTVRGIRR"
     gene            8572..10140
                     /locus_tag="VHM48_14680"
     CDS             8572..10140
                     /locus_tag="VHM48_14680"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX2756712.1"
                     /translation="MTRPVAIRILVLAAVIGLVAQALLLGNLLGVNAPILAVALLGAA
                     FVLRPGGRRIDPLDRWLPAAAVAISLSIAIRADPDLLAIDLAAALALLGASIAAIAGE
                     AVTRRSAVRVVELGFTVIGWAGVGILRVSAATRRPDPGPDGERVLDRLPSWAGPVARG
                     ILIAIPLLFVFGSLFSAADVAFERLIGRLFTWNLDLGELPIRLSIAFLIAWGVAGLLA
                     VAAGALDRAADAVEPPPGEPLPQSLGAAALGLPDATRSDGPWPLPRLGAIESATILIA
                     VDVLFGVFVILQVAYLFGGLDTLAATGLPYAQYARSGFFELIWVALLAGGLLATVHAI
                     AARRTPALVGAGLALAGLTAVVLASALLRLRIYQDAYGWTELRFYVLATIVWLAIGIG
                     ITITLLYRDRMRWLLHGLAIAAVVVLAGINIVGPSRLIAEQNVARVVDPFLVPADGKS
                     GLDVTYAVRLGDDAIPALVAALPALDPADREELFRRLLLRRVTLDEASATGWPSWNLG
                     RELAREALATLPPR"
     gene            complement(10227..>10763)
                     /locus_tag="VHM48_14685"
     CDS             complement(10227..>10763)
                     /locus_tag="VHM48_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX2756713.1"
                     /translation="TEVGEQTDATEVTGQLVDLDARIRNLRASEIALVGYVEKAAKTS
                     DLLEIEARLTDTRGQIEQLTAQQANLSDQAAMATLTVTFGTELVAVTEAAAKWDPAAE
                     VDRASATLIGVGQGIASLLIVFAIVWVPILIGLAILSLVGLVVARRLGWRRPGGYPPI
                     GSTEPSAPAIPPAPAPEA"
BASE COUNT         1402 a         3829 c         3898 g         1634 t
ORIGIN      
        1 gggcaggctc gaattgttga gcgtcgacgg tcgcgaggtc ctgctcgacg gtgcccacaa
       61 ccccgccgga gcggcggcgc tcgccgcggc tctcgacgag ctgcggccgt tcctcggtgc
      121 cggccgggtg acgctgctca cggcctcgat ggcggacaag gacgtcggcg gcgtcgttga
      181 cgcgctggcc ggctcgacgg ccctggccga tgcgcggatc gtcgccaccg cggtcgcggt
      241 cccgcgggcg ctgccggccg acgagctcgc gcgtgcgtgg tcgcgtcgga gcggcgaacc
      301 ggccggggcc taccgaacgg tcgcagccga gcccgatgtc gacgcggccc tcgaccggat
      361 cctcgccgga ccgaccggcc cggtcgtcgt cgccggctcg ctctatctcg tcggcgcggt
      421 ccgggcccga ttggtcgacg acccgctcct gcacgatccg acgcccgatg acgcgtcgcc
      481 gcccgacgcc cgggaccggt cccgcccatc ggcggccgat cccggcgaca atccttcccg
      541 atgaccgatc ggagcccgac ccgcccggat gtcgtcgccg cccggcccga gctcggaggc
      601 ggcgcgacga cgccatcgtt cggcctgccg gccgacgccc cgacgggcat cccgggtccc
      661 gctgcccggc tccagatcgg gccggcgccg ttcgattggg gaacccggac gttcgtcatg
      721 gggatcctga acgtcacgcc cgattcgttc tccggggacg ggttgctcgc gtccacgcct
      781 ggcgcgaccg acccggaccc ggtcgctgcg gcggtggcga tcggccggcg gatggtcgat
      841 gagggcgccg acctgctcga cgtcggcggt gagtcgacga ggcccgggca cgaggtcgtc
      901 gccgacgatg atgagatcgc ccgcgtcgtc ccgatcgtgc gcgccctccg ggcagccctg
      961 cccgacacac cgctgagcat cgacaccacg aaggtcgcgg tcgcggcggc tgccctcgac
     1021 gccggcgcga acctcgtcaa cgatgtctgg ggggtcggcc aggacgacgc cctccttcgt
     1081 ctggcggccg ccgtgggcgc gcccatcgtg ctgatgcaca atcgtgctga ggcgcgctac
     1141 atgaacctga tggccgaggt cgtcgcggat ctccagcgtg cgatcgagcg agccattcgc
     1201 tgcgggatcg ccccggagtc gatcgtcatc gatccgggct tcgggttcgg caagaccccc
     1261 gcccacaacc tcgtcctgct ccgggacctg gcgatcctgc ggatcctcgg ccggccgatc
     1321 ctgctcggca cctcgcgcaa gtcgaccctc ggcaaggtcc tcgacctgcc cgccgaccag
     1381 cgcctcgagg cgacgctcgc gacgaccgcc ctgggggttg ccgcaggcgt cgacatcgtc
     1441 cgggtccacg acgtccgggc aaatgtccgg gcggcgcgga tggccgacgc gatcgtccgg
     1501 cgacccgggc catctgccgc ccacccgacc gaaggagacc agccctgagc gaccggatcg
     1561 tgctccacaa catccagctc cagggccgcc acggctacta cgaccatgag ctcgcggcgc
     1621 cgcagccgtt cgaggtcgat gtcgagctga tgctcaatct ccagccggcc gggatcgatg
     1681 acgacctcgc gaaatccgtc gactacgccc gcgtccacga ggtcgtccgc cagatcgtcg
     1741 agtcgacgtc ctaccggctc ctcgaggcac tggccgaggc gatcagccac gagatcctga
     1801 tcgagttccc ggttgtcgag gtcggcgtcc gggttcgcaa gccggccgtc cagctgggcg
     1861 gcccgatcga ctacgccggc gtcgagatct ggcggcgacg ctcgccgggt tgacccgccg
     1921 gaccccggaa gcccggcgtc ggcgtcgacg tcccgggccg gcccgtcagc tgaggtcctt
     1981 cggtcggacg ccgagggtga ccgcgacggt cttgtgctcc gtgccgcgga tcacctcgag
     2041 ggtgatcttg tcgcccggtg cgaactgggt caggacggcg tcgagcgggt ggagcgcgtc
     2101 gatcgtctgg ccgttgatcg cgatcacgat gtcgcccgtc ttgatcccgg cgttggctgc
     2161 cgggctgttc gggcggacgg cctccgctcc ggtcgggtcg ccgttctcgt cgaggagctg
     2221 gacgtaggcc ccggccgtcg ccggcagggt gaggtgcttg tagatctgcg ggtcgatcga
     2281 cacgtactgg atcccgatgt agggtcgggc gagggcctcg ccggcaaggg cctggcgcat
     2341 gatcgggcgg gcgatgtcga tcgggatcga gaagccgatg ccggtcgagt ccttggcgat
     2401 cgcggtgttg acgccgatga ccgcgccggt cgcgtcgagg agcgggccgc cgctgttgcc
     2461 gggattgatg gccgcgtcgg tctggaccag gttgcgcagg tggccaccgt cggtcgtgat
     2521 gtcccggccc agcgccgaga cgatcccgct cgtcacgctg ttgtcgaagg tgccgagggg
     2581 gctgccgatg gcgacgacga gctcgccgac cttcagtccg tccgaatcgc cgatcgtcgc
     2641 cgccggcagg ccggtcgcgt cgaccttgat gatcgcgagg tcggtcaggg tgtcgatgcc
     2701 gtagacggtc gcgtcgtact tggtcccgtc cttgagctgg accgtcaggt cggtcgtgcc
     2761 cgcgacgacg tgacggttgg tcaggatcca gccggcggag tcgaagatga tcccggaccc
     2821 gacgccctcc tgttcctgct gggtggccga gttcggatcg acgcccttgg tgtagatgcg
     2881 gacgaccgac ggaccggcct tggccgcgac atcgatgatc gccgagtttt cgtcgagcgt
     2941 gaccggttgc ttgatcgtgg tcgagttcgg gttctggctg cccgtcgcgg cgacgggcgt
     3001 tcgatcgagg gctccggtgg ccgtgagggc cgcgatggtc ccgcccgatg cgagcaccgc
     3061 ggcgacgagc gcggtcgcga ggacggtgcc ggtgcctgcg ccccggcgcg gcgtggtgac
     3121 gatcggccgg accggcacgg gcgtcgctgt ttcgaaccag tgctccggtg tcggctcccg
     3181 atcagaccag ctggtccggg tccagtcggt ccgcggctcg ggggtcggcg agaacgatgc
     3241 gggcccgatc agcggatcga cggcccggtc gtcacgtggc gggtcggcga ccgcgggcca
     3301 gccgtcgcgc acagccgatg ggttcgggga ggtcgttccg gccggggcgt ccgccgtcgc
     3361 ggcggcgcgt tcgaacgccg cgggtgttgc ctgtgccgcg ggcatgtccg ctgcggcggc
     3421 gcgatcgatc ggtcgccagg cggcggcatc gtgcggctga gtctcgtcgg tcggatcgga
     3481 gaagcgatcg gtcatcggga gcgtactcgc ctgcgggtgt ggttcgcggc tggtgcctcg
     3541 cgagtggcgg cgggagcggt ccgcctcgcg atcgtcgagg atctcagact accctgacgt
     3601 ctcgcggttc aggtggggcg ccccggtcgg tgaagaatcc accatcttcg ggagatccgt
     3661 gggaggttcc agcgtcgtcg atgcggtcgt cccgaggtcg tcctcgcggc gcgggtcggc
     3721 cggaaggttg acggtgaacg tcgttcctgc tccgagccgg ctgacgacct cgacggtgcc
     3781 acggtgcatg tcgacgatcg agcggacgat cgcgagaccg aggccgctgc cactgccgcg
     3841 ggcctcgttc gcccgcgaac cgcggtagaa ccgctcgaag atccggggca gctcggccgg
     3901 ctcgatgccg accccggtat cggcgacctc gatccgtgct ccgccgtccg cgaccgcggc
     3961 gacgctcacc gcgaccgatc cgccaggttc ggtgaacttg atcgcgttgc cgaccaggtt
     4021 cgtcaccacc tggccgatcc gctgcgggtc gtggcggagc cggatcgggg ccgtcgcggg
     4081 ctcgaggcgg agggcaacgc cccgccggcg cgccgccgga cccgcctgct cgaccgccga
     4141 ttcgacggcc gcccgcagat cgtccggacg caggtcgagg aggacgagcc cggaatcgag
     4201 ctttgagagc tcgagcaggt tctgggcgag ccagtcgagc cgctcgagct ggatccggct
     4261 gctgttgagg aactccgccc gcgcggcggg atcgtcgccg gtccgctcgg tgagcagctc
     4321 gttgaaggtc aggagcgcgg cgatcggcgt ccgcagctcg tgcgacacgt cggccaggaa
     4381 atcgcggctg cgatcgcggt cgcggcggat catcgcgacg ctcgcctcga gctggtccgc
     4441 catcgcgttg aactgggcgg ccagcgcggc gatctccgag gaaccggacc ggatgtcggc
     4501 ccgctggatc cgccttccga gctcgccctc ggcgagggcg cgcgaggcct cggtcatccg
     4561 ccgcagtggc gtcgtcacgc ggagggccag ggccgccgcg acgagaacgg cgatccccag
     4621 cgcaagcgcg cccacggcgg ccgtcacggt cgtcacgttg tcgatcgccg actgccggaa
     4681 ggtgtacggg ttcgagagcc ggacctcgat gacgtacgga aagtcgctct cggtcgagac
     4741 ccggatgagg ggagccgccc gcagcgtctc cttcgtcagg ccgggccggc tcggcgaccg
     4801 gaccgacgca tggaacgagc cgtcgatcgc cgcgacggtc gtcccggcgt cagccccacc
     4861 ggccgacggc aggcccagga cgatgtcgac gtcggcttcg gcgagccgat cggcaatgaa
     4921 gcgggcctgg gcgttcgatc cgagctcatc ggcgacgtct ggattgacgt ggttgtcggc
     4981 gaagaccacc ggcagcccct gcgccgaatc cttgttcagc gtgtcgacgt actcggacac
     5041 gagctgggcg cggacctgca gatcggcgat ctgctgggtc cggaactcgt cgtcgaggcg
     5101 gttgagcacg aagacagtga cgagcagcag gctcagggcg acgacgctcg cgaagccgag
     5161 ggtcagccga gcctggagcg acttcggccc ggcccgccgg atcatcctgg ggatgagccg
     5221 cggccggtgg aaccgtgagc gacggccggg ccgcccaacc gtcgtcgggt cggcgtcggc
     5281 gtcggcgtcg gcgtcggcgt cgggatcggc ggcgacaacg gagtcgaggt cgaccacctc
     5341 gaaacgatcc gggtcgttca cgtcccggtc cgggtgcggg ttcggtgaag ggtccgcggc
     5401 cgtgcccggg ctaccgctcg gcgaacgcat agccgacgcc acggaccgtc atgacgaacg
     5461 ccggcaccgc ggggtcgtcc tcgatcttgt cgcggaggtg gctgacgtgg acgttgatcg
     5521 ttttctcgtc ctggtcgaga gcctcgtagt cgcggaggag ctcgaggagc tcgccccggg
     5581 tgtacacccg accgggtcgc gaggcgagat ggcggaggat ctcgaattcg gtcggggtga
     5641 ggctgatccg gcgatcgccg cgctggacgc ggcgtcgctc gagatcgatg acgaggtcgc
     5701 catggcggat cacccgcccg ccggcggcat cgctcaggtc gccgtacgcg cgccggagga
     5761 gggccttgat ccggctcatg agctcgcgca ggccgaacgg cttggtcagg tagtcgtcgg
     5821 cgccgagctc cagcccgatg accttgtcga cctcgtcgtc gcgggcggtc aggacgagaa
     5881 ccggggcctt cgagccggac gcccgcagcc ggcggaggac ctcgaagccg tcgatcccgg
     5941 gcaggcggac gtccaggacg acgaggtcgg gcgcctggtt cgtggcgaag tcgaggcctt
     6001 cctcgccgct cttggcgacc gcgacctggt agccctcctg ctggagggcg tactggatcc
     6061 cgcgggcgac ggcgaactcg tcctcgacga tcaggatcgt ggcactcatg gccgcccgac
     6121 tgtacaccgg gcaggcacga accgggcgag ccgcctcgtg gctcccctcg tgataaggtg
     6181 tgcgcccgcg ccgggcgccc cgaccgcacc gtcggcgagt cgcgcccgca ccagaccgcc
     6241 caccgccgag gttccgcccc gatgtccacc gccacgacca cccgcctgaa cctcgccgcc
     6301 gagccgcgcg agctggtcgg caagaaggtt gccactctcc gccgcgtcgg ccgcctgccc
     6361 gccgtcgtct acggccacgg tgtcagctcg gagaacgtgt cgctcgacgc ccacgatttc
     6421 gagctcctcc gccgcaaggc cggccagaac gagctgatcg acctgtcggt cgacggcaag
     6481 aaggcccgcc ccgtgctcgt ccactccgtc caggtccatg cggtcaaccg ccgtccgctc
     6541 catgtcgacc tgttcctcgt ccggatgacg gaagagctga cggtcgacgt gccgctcgtc
     6601 gcgatcggga cctcgaacgc cgtcgagaac ctcaacggca cgctcatgca ccagctcgag
     6661 tcggtcagga tcaaggcgct gcccgaccat ctcccgcagt cgatcgagta ctcgatcgac
     6721 tcgctcgtcg acttcgacac ggtcgtcaag gtgtccgacc tggccattcc cagcgacgtc
     6781 acgctcctga ccgatgccga cgaggtcgtc gccaaggtgc tcgcgtcgcg gctccaggcc
     6841 gaggccgccg agctcgctgc cgagaccgcc gagaccgtgg ccgaggctgc cgagaccgcc
     6901 gagggcggcg aggccgccga gggcgcgacc gaagccgcga ccgatggtgg cgactccgcc
     6961 gagagctgag ccggcgggtc cgcggactcg ttgtgcgggc gccgccgcgg actcgttgtg
     7021 cgcgcagcgc caaggacgcc cgccgccagg cgtcgctgcg gcggggcgcc tgcagccggg
     7081 acaactctcc gcccggactg cgcatcagct tgcgagccgc cgtctcgcgg acggtgctcc
     7141 gaggaaccta ccggtcgtcg acggtcaggg tgcggtccgt gaaccccgcg tcggatgcgt
     7201 cgaccagctc gatccggttg ccgaacgggt cggccgtgta gcaccgaatg agtccggtgc
     7261 cggcgtcgtc ctcggtcaat gcggcaccgg ccgccacgag ggcgcggcgg gcggcgtcga
     7321 ggtcggtgac gatcaggcag ggatgggcct tcttcgcggc gatgaagcgg tcatcgaccc
     7381 cgaggtggat ggccgtcgcg ccaggtccga cgaaccagca gccgccgcgg cccctcagcg
     7441 tgtccggctt ggcgacctcg cgcagcccca gcacggtccc atagaagcgg cgcgcctcgg
     7501 cttcgcccct gacgggcatc gccagctgga cgtgatcgaa gccgagcacg ccgatgcccg
     7561 gcgggtcggc ggtcgtcaac gtcggatccc ccggacggtg accgtcagct gacgcatggc
     7621 cagctgatcg ggtcggtgtc gggccaggcg gcggcgaagg gcggcgtcga accgggcgcg
     7681 gacgtcgggc tccaatccgc ggaccaggtc ctcctcgtcg aactcggcca tgaagccgac
     7741 gtatccgtcg atgtcgaacg ggtggacgag ctcacccgcg gtggcgcgaa cggcggcgaa
     7801 cccggcacga cgcatctggg cggccgccgc cgccgcgtcg cgcaggtcgt cgtggtcgtc
     7861 gggccagccg cggggctcct cgccgacctc cacgagggca tcgtcgaagt cgacatcggg
     7921 tgcccagatg gtctcgtggg taagccacga gacccagccg aagacgccgc ccgggcgcag
     7981 gacgcgatgg gcctcgcgga gggcgcggaa gcggttgggc acgagctgca gcacgaacga
     8041 cgacaccgcc acgtcgatgc tgccgtcgcc gaggccgagg tcgtcggcga acgccacccg
     8101 cagctcgagg cggtcgcgct gggcggctgt cagcgagcgg tcggactcgg cggtggccat
     8161 ggcgatcatc ccgcccgacc cgtcgatcgc cgtgacccgg acccggggcc agcgccggag
     8221 gatggcgatc gccagtgtcc cggtacccgt tccgacatcg aggaccgacg tcgcgccggc
     8281 ctcgatcgcg tcggcgacct cgtcgaggat ggtcacggcg gccggcgcga tgatcggcgc
     8341 ccaccaacgg ccgtagccct cggcgatccg gtcgtagcgc tcggcctgct cggtcatcac
     8401 ggccatcgtg ccatgggtcg cccggcctga catgcgcaac acggcgcgac gcccggtcgt
     8461 ctgcgctgag cagcctgttc cgaagccgat ccctcgcccg ccgcgcgacc gatcgcccac
     8521 gcatcccacg gattgccgcc tcgccgatcg cggcgccaca cggagccctg catgacccga
     8581 cccgtcgcca tccggatcct cgtcctcgct gccgtcatcg ggctcgtcgc ccaggcgctc
     8641 ctcctgggca acctgctcgg tgtcaatgcg ccgatcctgg ccgtcgccct gctcggggcg
     8701 gcattcgtcc tccggccggg cgggcgccgg atcgatccgc tggatcgctg gctgcccgcc
     8761 gcggcggtcg cgatcagtct ctcgatcgcc atccgggcgg acccggacct gctcgccatc
     8821 gacctcgcgg cggcgctcgc cctcctcggc gcgtcgatcg cggcgatcgc cggcgaggcg
     8881 gtgacgcggc ggtcggccgt ccgggtcgtc gagctgggct tcaccgtgat cggctgggcc
     8941 ggcgtcggga tcctgcgcgt gtcggccgcc acgcgacggc ccgacccggg ccccgacggg
     9001 gagcgggtgc tggaccgcct tccgtcgtgg gccggaccgg tcgcccgcgg catcctgatc
     9061 gcgatcccgc tcctgttcgt cttcggcagc ctcttctcgg cggcggatgt cgcattcgag
     9121 cggttgatcg gccggctctt cacctggaac ctcgacctcg gcgagctgcc gatccgcctg
     9181 tcgatcgcgt tcctgatcgc gtggggcgtg gccggcctgc tggccgtcgc cgccggcgcg
     9241 ctggaccgcg ccgcggacgc cgtcgagccc ccgcccggtg agccgcttcc gcagtcgctc
     9301 ggtgctgccg cactcggcct gcctgatgcg acccgttcgg acgggccgtg gccgctacct
     9361 cgtctcggcg cgatcgagtc ggcgacgatc ctgatcgccg tcgacgtcct gttcggggtc
     9421 ttcgtcatcc tccaggtcgc ctacctgttc ggcgggctcg acaccctcgc cgcgaccggc
     9481 ctgccctacg cccagtacgc ccggagcggg ttcttcgagc tcatctgggt cgccctgctt
     9541 gccggtgggt tgctggcgac cgtccacgcg atcgcggccc ggcggacacc cgcgctggtc
     9601 ggcgcggggc tggcgctcgc cggtctgacc gcggtggtgc tcgcgtcggc actcctccga
     9661 ttgcggatct accaggacgc ctacggctgg acggaattgc gcttctacgt cctcgcgacg
     9721 atcgtctggc tcgcgatcgg gatcgggatc acgatcacgc tcctctaccg agaccggatg
     9781 cgctggctcc tgcacggact cgcgatcgcg gcggtcgtcg tcctcgccgg gatcaacatc
     9841 gtcgggccgt cgcgactgat cgccgagcag aacgtcgccc gggtcgtcga tccgtttctg
     9901 gttccggcgg acggcaagag cgggctcgac gtcacgtacg ccgttcgcct cggcgacgac
     9961 gcgatcccgg ccctcgtcgc ggcgctcccg gcgctcgacc cggccgaccg cgaagagctg
    10021 ttccgccggc tcctgttgcg gcgcgtcacg ctggacgagg cgagcgcgac cggttggccg
    10081 tcgtggaacc tcggacggga gcttgctcgg gaagcgctcg cgacactgcc gccgcggtag
    10141 ggcccgcagt cgcggcgcgg aatgcctggc tcattcctgc gagctggcgg aatccactcg
    10201 cctcgccgag acctggatgc gacccttcag gcctccggcg ccggagcggg cgggatcgct
    10261 ggtgcgctcg gctcagtcga gccgatcggc gggtacccgc ccgggcggcg ccagccgagt
    10321 cgacgggcga cgacgagtcc gaccagggag aggatggcca gtccgatgag gatcgggacc
    10381 cagacgatcg cgaacacgat caggaggctc gcgatgccct gcccaacccc gatcagcgtg
    10441 gccgacgcgc gatccacctc ggccgccgga tcccacttcg cggccgcctc ggtgacggcc
    10501 acgagctcgg tcccgaacgt gaccgtaagg gtggccatcg ccgcctggtc cgagaggttc
    10561 gcctgctgtg ccgtgagctg ctcgatctga ccgcgcgtgt cggtcaggcg ggcctcgatc
    10621 tcgagcaggt ccgacgtctt cgcggccttc tccacgtagc cgacgagggc gatctcgctg
    10681 gccctcaggt tgcggatccg cgcatcgagg tcgacgagct ggccggtgac ctcggtggcg
    10741 tccgtctgct cgccgacctc ggt
//
LOCUS       DATAIW010000497         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_203791, whole genome shotgun sequence.
ACCESSION   DATAIW010000497 DATAIW010000000
VERSION     DATAIW010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203791"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VHM48_14690"
     CDS             <1..549
                     /locus_tag="VHM48_14690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756714.1"
                     /translation="VGEGFQPPNEPTEIALDRFYSLLLETGERLQFSFGPREAFLAWW
                     RAAQRAGHLILLEARTADGEVQAGLVLYRHGERLSTVHSGDHAAARRDHPGALHLLRW
                     RAIELAIREGRTEMDLGGADVAGARREPREGEPMWGLYQHKRSFGGEWLELAGAHERV
                     LDPNRYRLGRIAAKAARLLGRR"
     gene            546..2192
                     /locus_tag="VHM48_14695"
     CDS             546..2192
                     /locus_tag="VHM48_14695"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX2756715.1"
                     /translation="MSDEATIASLMAAEGGGPQQLNGLVERLSAGGLLRGAREGGRAI
                     GPTALGEILVEDITDDSRAVRPGSLFVAVPGLHVDGHVFVASAAAAGAAAAIVERPLP
                     DVALPQLVVERAAAALAESAAWWFGDPSRRLGVVGITGTDGKTTTSFLAVAALEAADV
                     SCGLVGTVATQIGAVREANPEHTTTPGALLLQRTLRAMASADNDVAVVETTSHGLAAD
                     RVRGIAYDIAILTNLTHEHLEFHGSWEAYRDAKLSLFERLAVGPANPVKELGGRRWPK
                     GAIVNADDPNAGAFAGVAQEAGARIVTYGTDSGADVRATHIEEDARRLRIGYEAPSGA
                     AVLDLQLAGRFNVHNALAVVAMGEVLDLDPAAVRAGLAGVRGVPGRMERLAAGQPFGI
                     VIDYAHSPASLEKVLDLLAPLAAARGGGVIAVFGSAGERDTEKRPMMGLIAAERARLV
                     IVTNEDPRGEDPMTILEGIARGAEDGGKRRDRDLFLIADRRRAIEAAFERARPGDIVL
                     LAGKGHEQSIIGPDGPVAYDERQTALAVLAGLGFVPDPAT"
     gene            2194..>2546
                     /locus_tag="VHM48_14700"
     CDS             2194..>2546
                     /locus_tag="VHM48_14700"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HEX2756716.1"
                     /translation="MRGCLFVLVLGAAILGGAAWFGSPILASTVIATALQNAGYHAAT
                     STVTATSDPPPKLLLGRADQVEIRGTDVDFRAFHAASLDLVLTDVDVVGRTAARISGR
                     IVGAELATTDGTATTA"
BASE COUNT          348 a          939 c          916 g          343 t
ORIGIN      
        1 gtgggggagg ggttccagcc accgaacgag ccgacggaga tcgccctcga tcgcttctac
       61 agcctgctcc tcgagacagg ggagcggctc cagttcagct tcgggccgcg cgaggcgttc
      121 ctcgcctggt ggcgggcggc ccagcgggcc ggtcacctga tcctgctcga ggcccggacg
      181 gcagacggcg aggtccaggc cggtctggtc ctctatcgcc acggtgagcg gctctcgacc
      241 gtccactcgg gcgaccatgc ggcagcccgg cgcgaccatc ctggtgccct gcacctcctt
      301 cgctggcggg cgatcgagct ggcgatccgc gaaggacgaa cagagatgga cctcggcggc
      361 gccgacgtcg ccggggcccg tcgcgagcca agggaaggcg agccgatgtg gggcctctac
      421 cagcacaagc ggtcgttcgg cggggagtgg ctggagctcg ccggcgcgca cgagcgagtc
      481 ctcgacccga atcgctaccg gctcgggcgg atcgcggcga aagcagcccg gcttctcgga
      541 cggcgatgag cgacgaagcg acgatcgcgt cgctgatggc ggccgagggt ggcggtccgc
      601 agcaactcaa cgggctggtc gagcggctgt cggccggcgg cctcctgcgg ggtgcccggg
      661 agggcggacg ggcgatcggg ccgaccgcgc tcggagagat cctcgtcgag gacatcacgg
      721 acgactcccg agcggtccgc cccggatccc tgttcgtcgc cgtcccgggt ctgcacgtcg
      781 acggccacgt gttcgtggcc tccgccgccg ctgccggggc cgcggcggcg atcgtcgaac
      841 ggccgcttcc cgacgtcgcg ctgccgcagc tcgtcgtcga gcgcgcggcg gccgcactcg
      901 ccgaatccgc cgcctggtgg ttcggcgacc catcacgccg gctgggcgtc gtcgggatca
      961 cgggcaccga cggcaagacg acgacgtcgt tcctggccgt ggccgccctc gaagcggccg
     1021 acgtgtcctg cggcctcgtc ggcaccgtcg cgacccagat cggagcggtg cgcgaggcga
     1081 acccggagca cacgacgacc cccggcgcgc tgctcctcca gcggacgctc cgggcgatgg
     1141 cctcggcgga caacgatgtc gcggtcgtgg agacgacctc gcacgggctc gcggcggacc
     1201 gggtccgggg catcgcctac gacatcgcga tcttgacgaa cctgacccac gagcatctcg
     1261 agttccacgg ctcgtgggag gcataccgcg acgccaagct gtcgctgttc gagcggctgg
     1321 cggtcggacc ggcgaacccc gtcaaggagc tgggcggccg gcgctggccg aaaggggcga
     1381 tcgtcaacgc cgatgacccg aacgccggcg ccttcgccgg tgtcgcccag gaggccggcg
     1441 cccggatcgt gacctacggg accgactcgg gcgccgacgt ccgcgcgacc cacatcgagg
     1501 aggacgcccg gcgtcttcgg atcggctacg aggcgccgtc cggcgcagcc gtcctcgacc
     1561 tccagcttgc ggggcgcttc aacgtccaca acgcgctggc cgtcgtcgcc atgggcgagg
     1621 tcctggacct cgacccggcg gcggtccggg ccggcctggc cggtgttcga ggcgtccccg
     1681 gccggatgga gcgactcgcc gccggccagc cgttcgggat cgtcatcgac tacgcccaca
     1741 gcccggcctc gctcgagaag gtcctcgacc tgctggcccc gctcgctgct gcccgcggcg
     1801 gcggcgtgat cgccgtcttc ggctcggcgg gggagcgcga caccgagaag cgcccgatga
     1861 tgggcctgat cgcagccgag cgggcccgcc tggtcatcgt cacgaacgag gacccgcgcg
     1921 gcgaggaccc gatgacgatc ctcgaaggaa tcgcacgcgg cgccgaggat ggcggcaagc
     1981 gccgcgatcg cgacctgttc ctgatcgccg accgtcgccg tgcgatcgaa gccgcgttcg
     2041 aacgagcgcg gccgggcgac atcgtcctcc tcgccggcaa gggccacgag cagtcgatca
     2101 tcggcccgga cggacccgtc gcctatgacg agcgccagac cgccctcgcc gtcctggccg
     2161 ggctcggatt tgtcccggat ccggccacct gacgtgcgcg gctgcctctt cgtcctcgtc
     2221 ctcggcgccg caatcctcgg cggcgccgcc tggttcggct cgccgatcct ggcctcgacc
     2281 gtcatcgcga cggcgctcca gaacgccggc tatcatgcgg cgacctcgac tgtgacagcc
     2341 acgtcggacc cgccgcccaa gctcctcctc ggccgtgccg accaggtcga gatccgcggt
     2401 accgacgtcg atttccgggc gttccatgcg gccagcctcg acctcgtcct gaccgacgtc
     2461 gacgtcgtcg gccggacggc ggcccggatc agcggccgga tcgtgggcgc cgagctggcc
     2521 acgacggacg ggaccgcaac gaccgc
//
LOCUS       DATAIW010000498         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_233804, whole genome shotgun sequence.
ACCESSION   DATAIW010000498 DATAIW010000000
VERSION     DATAIW010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233804"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHM48_14705"
     CDS             complement(<1..197)
                     /locus_tag="VHM48_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756717.1"
                     /translation="MTHTTEALVGPAPLGRRRRFTSPSGREAIWGLVFVGPWIIGFLL
                     FTFGPLVASFVLSLTDFNLVRP"
     gene            complement(282..1625)
                     /locus_tag="VHM48_14710"
     CDS             complement(282..1625)
                     /locus_tag="VHM48_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756718.1"
                     /translation="MLISAPPIAAGPGPNGGVVVRWFIGLGAGTQPAQIGPEQAFITA
                     YNASQKDVYIQYEIVDNTQAANILKTEIAAGNAPDIIGPVGVEGLNLFTDQLLDLKAL
                     IQSTGYTSTGVDQPLVDFFNSLGQNGATVGLPFAVYPSFIFYNKDLFDEAGLPLPPTK
                     VGDTYDGKPWDLDALRTLSMKLTVDKNQKDATEAGFDPTNIVQWGFDSQYMDNYNARA
                     ETAFFGSGALLAADGKTAVVPDYIKQGEKWYNDGVWKDHFIPTAAQVASDLLSGGSEF
                     GSGNLAIDEGHTWFTCCVTPAAPKKAFKFGFAVNPTVNGKIVSPLHADTFSVLKTTKV
                     PDAAFKALTAMVASVDLLTAYGAMPADQTKQQAWFDSIDKNFPGMRLNWDVAKAMLAY
                     PDVPNHQSFVPDYAKVRSAMQAFGNNYRTTSGVDMDAELTKLQATLQGIFDAATP"
     gene            complement(1901..3001)
                     /locus_tag="VHM48_14715"
     CDS             complement(1901..3001)
                     /locus_tag="VHM48_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2756719.1"
                     /translation="MLTCVLSVRALSWRTQLTVSNTQPRPRGVTLEEVARLAGVSRAT
                     VSRVVNGSPRVSGDVRRSVETAVEKLGYVPNRAARSLVTRRSGSIAVVITEPTGRLFS
                     DPFFPRLLRGISTTVAARDVQVVLLMPETIGDSQRTADYLVAGHVDGAILVSLHGDDP
                     LPGRIIDARIATVVVGRPLRNRGVSYVDVDNRGGARNAVEDLIAGGRRVIASIGGPAD
                     MMASQDRLHGYRDALVAAGLGADPTLEAAADFTQDGGAAAMRQLLAARPDLDAVFAAS
                     DLMAAGAMSVLDAAGRRVPEDVAIVGYDDSPVAATARPPLTSVRQPIEEMGQESARLL
                     LELVEDADRAPRRVILTTELIRRASSAGRAMP"
     gene            3151..>4555
                     /gene="gatB"
                     /locus_tag="VHM48_14720"
     CDS             3151..>4555
                     /gene="gatB"
                     /locus_tag="VHM48_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX2756720.1"
                     /translation="MTATPAASGLEAALERYEAVIGIEVHCQLKTASKMFCGCSNAYA
                     DAPPNTHTCPVCLGLPGTLPVINRRAVEHVLATGIAIGAETPPATRWDRKNYFYPDLP
                     KGYQISQYDLPLAAHGRLTFGTSEGEVTIGITRAHLEEDTAKLVHGTDETGGRISLID
                     FNRSGAPLMEIVTDPDVRSAEQARRYAEELQALLRAIGASDADMERGQMRVEANVSLR
                     PRGTEPFGIRVEVKNMNSFRSVERAIAYEIERQAAALDAGETLTQDTRGWNEDRGTTY
                     VMRSKEDSHDYRYFPEPDLPPLRVDAGWLASIRESLPELPAARRARYEANLGLSAYDA
                     AVIVGDGAATALFEGARAADPAADPKALANWVTGAFLGLLRTDPGAAARIDPAQLADL
                     VGRVTAGELSGTNAKEVFAAHAADGATMAAIVDARGFRQISDTGALAAIVDEVVAANP
                     KALDDYRAGKPVLGFFVG"
BASE COUNT          700 a         1525 c         1640 g          690 t
ORIGIN      
        1 ggacgaacca ggttgaaatc ggtcagggag aggacgaacg aggcgacgag cggcccgaac
       61 gtgaacagaa ggaagccgat gatccagggc ccgacgaaca cgagtcccca gatcgcctcg
      121 cggcccgacg ggctggtgaa cctgcgtcgt cggccgagcg gggcgggtcc aacgagcgcc
      181 tcggtcgtgt gggtcatggc tggatgggct ccaatccgga gagagtaggc cccctcgggc
      241 ctgggggacg ccgagggggc caggagtgag gaggagccgt cttacggggt cgccgcgtcg
      301 aagatgcctt ggagggtcgc ttggagcttc gtcagctcag cgtccatatc gactccggac
      361 gtggtccggt agttgttgcc gaaggcctgc atagcgctcc ggaccttggc gtagtcgggg
      421 acgaatgact ggtggttggg gacgtccgga taggcgagca tcgcctttgc cacgtcccag
      481 ttgagcctca tgcccgggaa gttcttgtcg atcgagtcga accaggcctg ctgcttggtc
      541 tgatcagcgg gcattgcgcc gtacgccgtc agcaggtcca cggaggcgac catcgccgtg
      601 agggccttga acgccgcatc cggaaccttg gtcgtcttga ggacgctgaa cgtgtccgca
      661 tgaagcggtg agacgatctt gccgttgacc gtggggttca cggcgaaccc gaacttgaag
      721 gccttcttgg gggccgccgg cgtcacgcag cacgtgaacc acgtgtggcc ttcgtcgatg
      781 gccaggttgc ccgatccgaa ctcgctgccc ccgctgagca ggtcgctcgc gacctgggcg
      841 gcggtcggga tgaagtggtc cttccagacg ccgtcgttgt accacttctc gccctgcttg
      901 atgtagtcgg ggacgaccgc ggtcttgcca tcggcggcaa gcagagcacc actgccgaag
      961 aacgccgttt ccgcgcgggc gttgtagttg tccatgtact gggagtcgaa cccccactgg
     1021 acgatgttcg tcgggtcgaa gccggcttcc gtcgcgtcct tttggttctt gtcgacggtc
     1081 agcttcatcg agagggtgcg gagcgcgtcg agatcccagg gcttgccgtc gtacgtgtcg
     1141 ccaaccttgg tcggcgggag cggcagcccg gcctcgtcga agaggtcctt gttgtagaag
     1201 atgaacgagg ggtagacggc gaacggcaga ccaaccgtcg ctccgttctg cccgagcgag
     1261 ttgaagaagt caaccagggg ctggtcgacc ccggtcgacg tgtatcccgt cgactggatc
     1321 agcgccttga gatcgagcag ctgatcggtg aacaggttga gaccctcgac gccgaccggg
     1381 ccgatgatgt ccggggcgtt gccggctgcg atctccgtct tgaggatgtt cgccgcctgg
     1441 gtgttgtcga cgatctcgta ctggatgtag acgtccttct gtgacgcgtt gtacgcggtg
     1501 atgaacgcct gctccggccc gatctgggcg ggctgggtgc cggctccgag gccgatgaac
     1561 cagcggacga cgacgccgcc attcgggccc gggcccgctg cgatcggcgg cgcactgata
     1621 agcaccgtcg acggggacgg gagcgccgtc tgcgtggcgg cggacggcgt ggtcgccgac
     1681 gctgaagggg acggggttgc ggacgcgttg cacgcgctgg ccagcaatac gacaagagcc
     1741 cccatggcga ggaccttgtg cggccctgat cctggacgtt tcattgcggc ggtctcctcc
     1801 tctacgagat ctcgttcgat ctccacctct ggtggtggac gaagggttgg tgggcgtggg
     1861 ctccgacctc acggtcgagg tccgccgggc gccggccggc tcagggcatc gccctcccgg
     1921 cgctggacgc ccggcggatg agctcggtgg tcaggatcac ccggcggggc gcccgatcgg
     1981 catcctcgac cagctcgagc aggaggcggg ccgattcctg gcccatctcc tcgatcggct
     2041 ggcggacgct cgtcaatggc gggcgggcgg tcgctgcgac cggcgaatcg tcatagccga
     2101 cgatggccac gtcctcgggg acgcgccggc cggcggcgtc gaggaccgac atcgcgccgg
     2161 cggccatcag gtcggaggcg gcaaacacgg cgtcgaggtc cggccgtgcc gcgaggagct
     2221 ggcgcatcgc cgcggcgccg ccgtcctggg tgaagtccgc ggcggcctcg agggtcggat
     2281 cggctccgag gccggccgcg accagcgcgt cgcggtagcc gtggaggcga tcctgggagg
     2341 ccatcatgtc agccggtccc ccgatcgagg cgatgacccg ccggccgccg gcgatcaggt
     2401 cctcgactgc gttccgggct ccgccgcgat tgtcgacgtc gacgtagctc acgccgcgat
     2461 tgcggagcgg acgcccgacg accacggtcg cgatccgcgc gtcgatgatc cggccgggca
     2521 gaggatcgtc gccgtggagg ctgacgagga tcgcaccgtc gacgtggccg gcgaccaggt
     2581 agtccgcggt ccgctggctg tcgccgatcg tctccggcat gagcaggacg acctggacgt
     2641 cgcgggcggc gaccgtcgtg ctgatgccgc gcaggaggcg cgggaagaac ggatcgctga
     2701 acaggcggcc ggtgggctcc gtgatgacga ccgcgatcga tccgctccgg cgggtgacca
     2761 ggctgcgcgc ggcacggttc gggacgtagc cgagcttctc gaccgcggtc tcgaccgagc
     2821 gccggacgtc accgctcacg cgaggcgagc cgttcacgac gcgggacacg gtcgcgcgcg
     2881 acacgccggc caggcgagcc acctcttcga gagtcacacc ccgcggccgg ggttgcgtgt
     2941 tggagacggt caactgcgtc ctccatgaga gcgctctcac gctcaacacg cacgttagca
     3001 cgccatgtca agagagcgct ctcacacatg cgcattgccg ctgggcgaca ggccacgggc
     3061 ggcgcggtcg cgccgcgtgc ccagcggacc cgtgcggccc gtccgggagc ccgtatgcga
     3121 aactgggccg atgcagcaca gcgttcgccc gtgaccgcga caccggccgc ctcgggtctc
     3181 gaggccgccc tggagcgcta cgaggccgtc atcgggatcg aggtccactg ccagctcaag
     3241 accgcctcga agatgttctg cggctgctcg aacgcctatg ccgacgcgcc gccgaacacc
     3301 cacacctgcc cggtctgtct cggcctgccc gggacgctcc cggtcatcaa ccggcgcgcg
     3361 gtcgagcacg tcctggcgac gggcatcgcg atcggcgccg agacgccgcc ggccacccgc
     3421 tgggaccgca agaactactt ctacccggac ctcccgaagg gctatcagat cagccagtac
     3481 gacctgccgc tggccgccca cggccgcctg acgttcggga cctcggaggg ggaggtcacg
     3541 atcgggatca cccgggccca cctcgaggag gacacggcca agctggtcca cggcaccgac
     3601 gagacgggtg gccggatcag cctgatcgac ttcaaccgct ccggtgcgcc cctgatggag
     3661 atcgtcaccg accccgacgt ccggtcggcc gagcaggccc gccgctacgc cgaggagctc
     3721 caggccctgc tccgggcgat cggcgccagc gacgccgaca tggagcgcgg ccagatgcgg
     3781 gtcgaggcca atgtctcgct ccggccgcgc ggcaccgagc cgttcgggat ccgggtcgag
     3841 gtcaagaaca tgaactcgtt ccgctcggtg gagcgggcga tcgcctacga gatcgagcgc
     3901 caggcggcgg ccctcgacgc gggcgagacg ctgacccagg acacccgggg ctggaacgag
     3961 gatcgcggga cgacctacgt catgcgatcc aaggaggaca gccacgacta ccgctacttc
     4021 ccggagccgg acctgccgcc gctgcgggtc gatgccggat ggctcgcctc gatccgcgaa
     4081 tcgctgccgg agctcccggc ggcccgtcgc gcccgctacg aggcgaacct cgggctgtcc
     4141 gcatacgacg cggccgtgat cgtcggcgac ggcgcggcaa ccgccctgtt cgagggggcg
     4201 cgcgcggcag acccggccgc cgatccgaag gcgctcgcga actgggtgac cggcgcgttc
     4261 ctcggcctgc ttcggacgga ccccggggcg gcggcacgga tcgacccagc ccagctggcc
     4321 gacctggtcg gccgcgtgac ggccggcgag ctgtcgggca cgaacgcgaa ggaggtcttc
     4381 gcggcccacg ccgccgacgg cgcgaccatg gcggcgatcg tcgacgcacg cggattccgc
     4441 cagatctcgg acacgggcgc gctcgccgcg atcgtcgacg aggtcgtcgc ggccaacccg
     4501 aaggcgcttg acgattaccg tgccggcaag ccggtcctcg gcttcttcgt cggcc
//
LOCUS       DATAIW010000499         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_204065, whole genome shotgun sequence.
ACCESSION   DATAIW010000499 DATAIW010000000
VERSION     DATAIW010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204065"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VHM48_14725"
     CDS             <1..779
                     /locus_tag="VHM48_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2756721.1"
                     /translation="PGRGAARRRVVALALGAVIVLVTIVPLVAVLLTALTRAVGLAPV
                     PANWTLANFAEAVDGRFVDGLVHSFGLAVAAATIVLPLGGLLAVIGRRGRGGGALRTA
                     ATMGFALPGSALAIGVLLAYGGWLRDTLAIILVAYVAKFWALGQRQVAGSVDRLPVDA
                     VRAARASGAGSIDALRSIVGPLLRPALVAGWLVVFLFALHELTMSSLLYGPRTTTLSV
                     VVLNAQQLGDPTVSAALAVLLVGLIGVVAVPLAIVARRRS"
     gene            776..1858
                     /locus_tag="VHM48_14730"
     CDS             776..1858
                     /locus_tag="VHM48_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756722.1"
                     /translation="MTAPGASGLVVEARRISVGYDRTLVVDGLDLTVAAGEMVVLLGP
                     SGSGKTTILSAIAGFLPIRSGALWIDGQVVAAAGRHVPPERRNVAVVFQGSALWPHLS
                     ALDTVAYPIRRRGAAMADARREARAILARLGIAHLADRRPAEMSGGEQQRVGLGRAVA
                     RGAALHLFDEPTAHLDGPLRERLLDEIAATRRATGAAGIYATHDTAEALAIADRVALI
                     RDGRLVQVGPPIEVYEAPVDGWAARLTGPASELDAVVVGPIGDLTEIDVAGVRVRAVI
                     DGPEAGVVPSPARLLVRPDWATLGGPLPGVVAGVRFRGSHTDIELATPAGDLTIRRAG
                     PPSVSAGDEPGWTLVHGRRLRDEPTA"
     gene            1980..2537
                     /locus_tag="VHM48_14735"
     CDS             1980..2537
                     /locus_tag="VHM48_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756723.1"
                     /translation="MTYQDPGRARPRNMRMLVLSLVLAFTAVGCVHASVPNADRRRDD
                     AQRLEAVLPTLQSLRVEGFRSQDWCRFIDEPRGAFTNIRDVTSVCNVFTKPPQAFDDS
                     ASTDFETVKQALSDSGVSTMMVWNIEYDGAGQITAAQFDLNAGSFDRFSYLYSRDTPV
                     ARDSFQAIVFEKINDHWWFLSDDFN"
BASE COUNT          315 a          944 c          960 g          419 t
ORIGIN      
        1 tccccggacg aggcgcggcg cggcggcggg tcgtggcgct ggcgctcggg gccgtcatcg
       61 tcctggtcac gatcgtgccc ctcgtcgcgg tcctcctgac cgcgctgacc cgggcggtcg
      121 ggcttgcccc ggtgccggcc aactggaccc tggccaactt cgccgaggca gtggacggcc
      181 gcttcgtcga tggcctggtc cacagcttcg ggctggcggt cgcggcggcc acgatcgtgc
      241 tgccgctggg cgggctgctc gcggtcatcg ggcggcgcgg acgtggtggc ggtgcccttc
      301 gaacggcggc gacgatgggg ttcgccctgc cgggatcggc gctggcgatc ggggtcctcc
      361 tggcgtacgg cggctggctg cgcgacacgc tcgcgatcat cctcgtcgcg tatgtcgcga
      421 agttctgggc gctcggtcaa cgccaggtgg cgggttcggt cgatcgcctg ccggtggatg
      481 ccgtccgtgc cgcccgggcg agcggcgccg ggtccatcga cgcgctccgt tcgatcgtgg
      541 gtccgctcct gcggccagcg ctggtcgccg gctggctcgt cgtcttcctc ttcgccctgc
      601 acgagctcac gatgtcgtcg ttgctgtacg gcccgcggac caccaccctg tcggtcgtgg
      661 tcctcaacgc ccagcagctc ggcgatccga ccgtgtcggc ggcgctggcc gtgctgctcg
      721 tgggtctcat cggggtggtc gccgtgccgt tggcgatcgt cgcgaggcgc cggtcgtgac
      781 ggcgccgggc gcgagcggcc tcgtcgtcga ggcccgccgg atctcggtcg gctacgaccg
      841 gacgctcgtc gtcgacggcc tcgacctgac cgtcgccgcc ggcgaaatgg tcgtcctgct
      901 cggaccgtcg ggctccggca agacgacgat cctgtcggcg atcgcgggct tcctcccgat
      961 ccggtcgggc gcgctgtgga tcgacggtca ggtcgtcgcc gcggcgggtc gccacgtgcc
     1021 gccggaacgc cgcaacgtcg cggtcgtgtt ccagggctcg gcgctgtggc cccatctctc
     1081 ggccctcgac accgtcgcct acccgatccg gcgccgcggc gccgccatgg cggacgcccg
     1141 gcgcgaggcc cgggccatcc tcgcccgcct ggggatcgcc cacctcgccg atcggcgacc
     1201 ggccgagatg tcgggcggcg agcagcagcg ggtcgggctc ggccgcgccg tggcccgcgg
     1261 cgcggcgttg cacctgttcg acgagccgac ggcccacctc gacggcccgc tgcgcgagcg
     1321 gctgctcgac gagatcgcgg cgacccggcg ggcgaccggc gctgccggga tctacgcgac
     1381 ccacgacacc gccgaggcgc tcgcgatcgc cgaccgcgtc gccctgatcc gggacgggcg
     1441 gctcgtccag gtcgggccgc cgatcgaggt ctacgaggcg ccggtcgatg gctgggcggc
     1501 ccggctgacg gggccggcga gcgagctcga cgcggtcgtc gtcggtccga tcggagacct
     1561 gaccgagatc gacgtcgccg gggtccgcgt ccgcgccgtc atcgacggcc cggaggccgg
     1621 ggtcgtcccg agtccggcgc gactcctggt ccgccccgac tgggcgaccc tcggcggtcc
     1681 gctgcccggt gtcgtggcgg gcgtccggtt ccgcggcagc cacaccgaca tcgaactggc
     1741 cacgccggcc ggcgacctga cgatccgccg ggcgggtccg ccgtcggtat cggccggaga
     1801 cgagccgggt tggacgctgg tccacgggcg ccggctccga gacgagccga cggcgtgaac
     1861 ttccgggccg gttcgtcagg tgcccgcgat caatcgacgg gtcggcgagc ggccgtgctg
     1921 tcgccggacg ccgctcgagc accaaatcgc cggcctcgat cggcgaggcg cacgggatcg
     1981 tgacgtatca ggaccccggc cgggcgcgtc cccgaaacat gcggatgttg gtgctgtcgc
     2041 tcgtcctcgc cttcacggcc gtcggctgcg tccatgccag cgttccgaat gcagatcgaa
     2101 gacgcgacga cgcccagcgc ctggaagccg ttcttccgac gctccagagc ctgcgggtcg
     2161 agggcttccg tagccaggac tggtgcaggt tcatcgacga gccacgaggg gcgttcacga
     2221 acatccgcga cgtgacgagc gtctgcaacg tgttcacgaa gccgcctcag gccttcgacg
     2281 actccgcttc gacggacttc gagaccgtca agcaggcgtt gagcgacagc ggcgtcagca
     2341 cgatgatggt ctggaacatc gagtacgacg gtgccgggca gatcacggcg gcgcagttcg
     2401 acctgaacgc cggatccttt gaccgcttct cctacctgta cagccgtgac actccggtcg
     2461 cgagagacag tttccaggcg atcgttttcg agaagatcaa cgatcattgg tggttcctgt
     2521 ccgacgactt caactgatcg gcgaagcatc cgccgcgcac cggtcagcgc gcggcacccg
     2581 tcacgtttcg aagctgcgca ggtgttcctc gtcgtgctcg atcgctcggg tcatcagg
//
LOCUS       DATAIW010000500         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_138818, whole genome shotgun sequence.
ACCESSION   DATAIW010000500 DATAIW010000000
VERSION     DATAIW010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138818"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..395)
                     /locus_tag="VHM48_14740"
     CDS             complement(72..395)
                     /locus_tag="VHM48_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756724.1"
                     /translation="MKKISRLPHEQAIADGDSGFGPEHDGLPVDQDVEGHQRQVADQF
                     LPGMPGTGGDNLHRPISGGEIDGDDVEGHLMGHTKGERLSPGMPGTGGDNIAVEVDPE
                     GVINR"
     gene            complement(668..1309)
                     /locus_tag="VHM48_14745"
     CDS             complement(668..1309)
                     /locus_tag="VHM48_14745"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2756725.1"
                     /translation="MTAGADPGSFDADDGLEPGEPVGGRPSATGPFHVAVVDAPWGPV
                     HVAATRQAILGIAVLTPREPFITDVMRRTGCDPAPGAGNPLLERAVAALRSFLAGDPA
                     MLESLPLDLADRGAWDRNVLGGVRTLRWGEVTSYGRLARAIGRRGAARAVGGAVGRNP
                     IGLAIPCHRVIAGDGTIGGYGGDWFGSRDELLAIKRELLAMEDVALPARSLRD"
     gene            1354..1869
                     /locus_tag="VHM48_14750"
     CDS             1354..1869
                     /locus_tag="VHM48_14750"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX2756726.1"
                     /translation="MESRRLPPGVLGVMPGPGQESVWDYPRPPRVEPVPVRIRAIVGG
                     RPIADSVRALRVVETAGAPVYYVPPADVDLGRLRPTDRTTVCEWKGPATYLDLVDPER
                     RIAAIGWTYRDPNPGYEAIRDCLAFYATKVDEAWVGDERATPQPGGFYGGWVTSTIVG
                     PIKGEPGSWGW"
     gene            1975..2214
                     /locus_tag="VHM48_14755"
     CDS             1975..2214
                     /locus_tag="VHM48_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756727.1"
                     /translation="MVPESEQRPTLVLNPPDDEDFRSSAERLVESGIVEPTELQESLR
                     LRWPNAVVRRRELAGERVQIWYVYRDGHWVRSAGS"
     gene            2255..2506
                     /locus_tag="VHM48_14760"
     CDS             2255..2506
                     /locus_tag="VHM48_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756728.1"
                     /translation="MDHDTAGRPDDERDSADPARQDLNATEDAIRADIQRLAAVEDEK
                     SALDAEDPDVDALSNEAVELAGRIHRETRAERQLSDEIG"
     gene            2569..>4824
                     /locus_tag="VHM48_14765"
     CDS             2569..>4824
                     /locus_tag="VHM48_14765"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2756729.1"
                     /translation="MTSDAVAPPSTSPPTASPVVEVELPIAGMTCASCVNRIERFLTK
                     SDGVESASVNLATEVATIRYLPEQTGRADLTRTIAAAGYDLKPPPTDEEHAAQRTLRT
                     AAEADDRRRAAEAARLLRDAAIAIVTAVAIMVAMFWPQTSLPMESINRLVLIPATLVQ
                     AWAGRRFYAAAWRAGRHGGATMDTLVAVGTTAAWAYSVVVTLNPAWVHEAGLHPETYF
                     DSSTIVLGLVLLGRWLEARARTRASGAIRRLIGLQATSALVIEAGGDRPLPIEEIQPG
                     DLLRVRPGDRLPVDGLVVDGGSAVDESMLTGEPVPVVKTAGDEVFGATVNTTGTFAFR
                     ATRVGADTALARIVSLVERAQGSKAPIQRLADRISEIFVPGVLGIAALTFVAWYLFGA
                     EPRLTLALTSFIGVVIIACPCAMGLATPTAIMVGTGRGAEAGILFRGGAALERAHRID
                     TVVFDKTGTLTAGRPTAGDIEPVAGWSAAELLDLSASLETGSEHPLGAAIVARARLDE
                     LGFRPVTAFEATAGHGVEGLVDGRRVVVGTARLLGDRGIDPGALRDRADAFAADGRTP
                     VWIGVDGELAGVVAISDPVKPEARPAIAELQAAGIDVWLVTGDHARTAAALAAQVGIR
                     PERVRAEVVPADKAAAVAALQADGRVVAMVGDGINDAPALAGADVGIAIGSGADVAIE
                     AAGITLVGGDPRGVPAAIALSRATMAVVRENLFWAFAYNVILIPVAMGLLVPFGIALS
                     PALAAAAMALSS"
BASE COUNT          640 a         1755 c         1727 g          702 t
ORIGIN      
        1 atccggcacc aacccgaccc tcctcggggc ggcggggtct ccgccccgag gagggtcggg
       61 ttggtgccgg atcagcggtt gatgacgccc tcgggatcga cctcgaccgc gatgttgtcg
      121 ccgcccgtgc ccggcatccc ggggctcagg cgctcgccct tggtgtggcc catcaggtgg
      181 ccctcgacgt cgtcgccgtc gatctcgccg ccgctgatcg gccggtgcag gttgtcgccg
      241 ccggtcccgg gcatgccggg caggaactgg tcggcgacct ggcgctggtg gccttcaacg
      301 tcctggtcga cgggcaaccc gtcgtgctcc ggcccgaagc cgctgtcgcc gtccgcgatt
      361 gcctgctcgt gcggcaggcg actgatcttc ttcatgacga gatcctccgg gatcgacccc
      421 gcttgctgtc cggtgctgtc cggtgcccat ccgtgatgtc gccaccatac caaggaggtc
      481 gtcggccgac cgatacctcc ggcgaccgga aggctgtcaa gtgcggcaca ccgatcgtgg
      541 acgccggagc cgcatccgcg accgcgatcg ccggggagac cgggccctgc tcagtagctc
      601 ttcggcaacg tctcggagct cgggcgcggc gtccggagat gcgaggcgtc cgccgctcgg
      661 ctgcctgtca gtcgcgcagc gaacgggccg gcagcgccac gtcctccatc gccagcaact
      721 cgcgcttgat cgccagcagc tcatcgcggc tgccgaacca gtcgccaccg tagcctccga
      781 tcgtgccgtc gccggcgatc acccggtggc acgggatcgc gagcccgatc gggttgcgcc
      841 cgaccgcgcc accgaccgcg cgggccgcgc cgcggcggcc gatggcccgg gcgaggcggc
      901 cgtagctcgt gacctcgccc cagcgcagcg tccggacgcc gccgagcacg ttccggtccc
      961 aggcgccgcg atcggccagg tcgagcggca gcgattcgag catcgcagga tcgccggcca
     1021 ggaacgagcg cagggctgcg accgcgcgct cgaggagcgg gttaccggct cccggcgccg
     1081 ggtcgcaccc ggtccgacgc atgacatcgg tgatgaacgg ctcgcgcggg gtcaggacgg
     1141 cgatccccag gatcgcctgc cgggttgccg ccacatggac cggtccccac ggggcatcga
     1201 cgaccgcgac atggaacgga ccggtcgcgg acgggcggcc gccgaccggt tcgccgggct
     1261 cgaggccatc atcggcgtcg aacgaaccag gatccgcgcc ggccgtcatc cgctcatctt
     1321 cggtcgtccg acgacccggc gtatgctcga tccatggagt cccgccgatt gccgcccggg
     1381 gtcttgggcg tgatgcccgg gccgggccag gaatcggtct gggactaccc gcggccgccc
     1441 cgggtcgagc cggtgcccgt ccggatccgg gcgatcgtcg gcggccgccc gatcgccgac
     1501 agcgtccgcg ccctgcgggt ggtcgagacg gcgggcgcac ccgtgtacta cgtcccgccg
     1561 gccgacgtcg atctcggtcg cctgcgtccg acggatcgga cgactgtctg cgagtggaag
     1621 ggccccgcga cctatctcga cctcgtggat ccggagcggc ggatcgcggc gattggctgg
     1681 acgtatcgcg acccgaatcc cggctacgag gcgatccgcg actgcctcgc cttctacgcc
     1741 acgaaggtcg acgaagcatg ggtcggcgac gagcgggcca cgccgcaacc gggcggcttc
     1801 tacggtggct gggtcacgtc gacgatcgtg ggcccgatca agggcgagcc cggttcctgg
     1861 ggctggtaag acgcccgacc gggcaggtgc aacacgcctg caagccacgc gcctcaccgg
     1921 tgcggcggtc cgacacgccg ggtcggccat cttgatcgtt ggtgagcgac caggatggtg
     1981 cctgaatctg agcaacggcc gacgctcgtt ctcaacccgc ccgacgacga ggactttcgc
     2041 tcgtcggcgg agcggctggt tgagagcggt atcgtcgagc cgaccgagct tcaggaatcc
     2101 ctccgcctcc gctggccgaa tgccgtcgtc cggcgtcggg agctggcggg cgagcgcgtc
     2161 cagatctggt acgtgtaccg tgacggccac tgggtgcgat cggctggctc ctgatcggcg
     2221 ttcgccaatc gcaccgtcct cccaggaggg acgcatggac cacgacacgg cgggccgacc
     2281 cgacgatgag cgcgactcgg ccgatcccgc aagacaggac ctgaacgcga cggaggacgc
     2341 gatccgcgcc gacatccagc gcctggccgc cgtggaggac gagaagagcg ccctcgatgc
     2401 ggaagacccg gatgtcgatg cgttgtcgaa cgaggccgtc gagctcgccg ggcggatcca
     2461 ccgcgagacc cgcgccgaac gccagctgag cgacgagatc ggctgatcct ctcctgacag
     2521 ccgcgtcggg ccggatcggc ggctctctct ggtatactcc ccgggagtat gacatccgac
     2581 gccgtcgctc ctccctcgac ttcgccgccg accgcctcgc cggtcgtcga agtcgagttg
     2641 ccgatcgccg gaatgacctg cgcctcgtgc gtcaaccgga tcgagcgctt cctgacgaag
     2701 agcgatggcg tcgagtcggc gtcggtcaat ctcgcgaccg aggtcgcgac gatccggtac
     2761 ctccccgagc aaaccgggcg ggcggacctc acccggacga tcgcggcggc gggctacgac
     2821 ctgaagccgc cgccgacgga cgaggagcac gccgcccagc gaacgctccg gaccgccgcc
     2881 gaagccgatg accgccgccg cgccgccgag gccgctcgcc tgctgcgtga tgccgcgatc
     2941 gccatcgtca ccgcggtcgc gatcatggtc gcgatgttct ggccgcagac ctcactcccg
     3001 atggagtcga tcaatcgcct cgtcctgatc cccgcgacgc tcgtccaggc gtgggccggc
     3061 cggcgcttct acgccgcggc gtggcgggcc ggtcgacacg gcggggcgac gatggacacg
     3121 ctggtcgccg tcgggacgac cgccgcctgg gcctacagcg tcgtcgtcac cctgaatccg
     3181 gcctgggtcc acgaggccgg tctccacccc gagacctatt tcgacagctc gacgatcgtg
     3241 ctgggcctgg tcctgctcgg gcgctggctc gaggcgcggg cgaggacccg ggcgagcggt
     3301 gcgatccgcc gcctgatcgg tctccaggcc acgtcggccc tcgtgatcga ggcgggtggg
     3361 gatcgcccgc tgccgatcga ggagatccag ccgggcgacc tgcttcgggt ccggcccggc
     3421 gatcgcctgc cggtcgacgg gctcgtcgtc gacggtgggt ccgcggtcga cgaatcgatg
     3481 ctgaccggcg aaccggtccc cgtcgtcaag accgccggtg acgaggtgtt cggagcgacg
     3541 gtcaacacga ccggaacctt cgccttccgg gcgacgcgtg tcggcgccga tacggccctc
     3601 gcccggatcg tctccctcgt cgaacgcgcc caggggtcga aggcaccgat ccagcgcctg
     3661 gccgaccgga tctccgagat cttcgtgccg ggcgtcctcg ggatcgccgc cctgacgttc
     3721 gtggcctggt acctgttcgg agccgagcca cgattgacgc tcgcgctgac ctccttcatc
     3781 ggcgtcgtga tcatcgcctg cccgtgcgcc atgggcctcg ccacgccgac tgcgatcatg
     3841 gtcggaacgg gccgcggggc cgaggccggg atcctcttcc gcggcggcgc cgccctcgaa
     3901 cgggcccatc ggatcgacac ggtcgtcttc gacaagaccg ggacgctcac ggcgggacgg
     3961 ccgacggccg gcgacatcga gcccgtcgcg ggctggtccg cggccgagct cctggatctc
     4021 tcggcatccc tcgagaccgg cagcgagcac cctctcggag cggcgatcgt cgcccgggcc
     4081 cgcctcgacg agctcggctt ccggccggtg acggcgttcg aggcgaccgc gggtcacggc
     4141 gtcgagggcc tcgtcgatgg gcggcgtgtc gtggtcggga cggcccgcct cctcggcgac
     4201 cgcgggatcg atccgggcgc cctgcgcgac cgggccgatg ccttcgccgc cgacggccgg
     4261 acaccggtct ggatcggggt cgacggcgag ctggccgggg tcgtcgcgat cagcgatccg
     4321 gtcaagccgg aggcccgccc ggcgatcgct gagctgcagg cggcgggtat cgacgtgtgg
     4381 ctcgtcaccg gcgaccatgc ccggaccgcg gccgccctcg cggcccaggt cgggatccgg
     4441 ccggagcggg ttcgggccga ggtcgtgccg gccgacaagg cggccgcggt ggcggccctc
     4501 caggcggacg gccgggtcgt ggcgatggtc ggcgacggca tcaacgatgc gccggccctg
     4561 gccggggccg atgtcgggat cgccatcggc agcggcgccg acgtcgcgat cgaggcggcc
     4621 ggcatcacgc tcgtgggcgg ggatccgcgc ggtgttccgg cggcgatcgc cctgtcgcgg
     4681 gcgacgatgg ccgtggtccg cgagaacctg ttctgggcct tcgcctacaa cgtgatcctc
     4741 atcccggtcg cgatgggtct cctcgtcccg ttcgggatcg ccctgagccc cgccctcgcc
     4801 gcggccgcca tggccctttc gtcg
//
LOCUS       DATAIW010000501        14374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_204594, whole genome shotgun sequence.
ACCESSION   DATAIW010000501 DATAIW010000000
VERSION     DATAIW010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204594"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..385
                     /locus_tag="VHM48_14770"
     CDS             137..385
                     /locus_tag="VHM48_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756730.1"
                     /translation="MNPLQFLADLETRPQWLGLLADRFARGNPFTAVPRDVERGRGPR
                     RGAARRDLVAPNRAHACMGRQQAGDESITLLTQRAAEQ"
     gene            551..1684
                     /locus_tag="VHM48_14775"
     CDS             551..1684
                     /locus_tag="VHM48_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756731.1"
                     /translation="MTTRKPTIRIAAAFAVFAMVLSACSSGTGGASAGTAATIPGVPQ
                     TLIDAAKTEGNLTTIALPHSWCDYGDLLSQFTSKFGIKINELNPKGGSKDEITALEAN
                     KTNKGPAAPDVVDVGLAYGPQGKAAGDFQTYKVAKWDTIPASVKDADGAWYGDYFGVL
                     AFEVNTAKVKNVPLDWSDLLKPEYKGQVALAGDPTVSNQAMSGVWAAALASGGSLDDA
                     TPGLAFFKKLNDAGNFVPIIAETANVASGDAAIRIAWTYNGLADRDSLKGNPPITVVV
                     PKSGRFGGVYVQAISAFAPHPNAAKLWMEWLYSDAGQNLYLKGYCNPIRYDDMVKNKT
                     VDATAQAALPDATGAVLPTLDQITKATAIVAKGWPTTVNVVVK"
     gene            1718..2599
                     /locus_tag="VHM48_14780"
     CDS             1718..2599
                     /locus_tag="VHM48_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2756732.1"
                     /translation="MATAPPLNLRGAPPARPGGRLSWSWLGVLPFFLFVLLFLVIPTL
                     YLLVGSFEDKAGSLTLQNYAHLSEPNLADSYFASIEISLVTAIAGGIFGFLVAYAVIL
                     GGLPSFLRTAIMTFSGVASNFAGVPLAFAFTFTLGASGILTPLIEALGVHFNIYSKTA
                     LELAYFYFQFPLMILIIAPAIDGLRKDWREAAENMGASSFQYWRHVALPILTPSLLGT
                     VILLFGNAFGAQATAFALSGGSINIVTIAITRQIRGDVANDPGVGYALAMGMVVIMAI
                     SIGIYSWLQRRSERWLR"
     gene            2587..3462
                     /locus_tag="VHM48_14785"
     CDS             2587..3462
                     /locus_tag="VHM48_14785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756733.1"
                     /translation="MAPMTGGTPLVAQAPKGPDSLAAAPVSRPRRSFGRIWWWAVFAV
                     AVLYFALPLIGTFAFSMRSQPLFSAYAVVTDDPQFAATLLYSFVVAIVTIIVSIVLIV
                     PTAFWVRLRLPRLRPYIEFITLLPFVIPPIILVFGLLRSYSGDPLPLAGSDFGSDVLL
                     VAAYVVLSLPYMYRAVDTGLRAIDVRSLTEASQSLGASWFTILVRVIFPNLRVSILSG
                     AFLTLAIVVGEFTIATFLARPAFGPYIQLLGGNKAYEPAAVSLISFGLTWIAMGLLAL
                     IGRQSRQKIQIAGAH"
     gene            3478..4578
                     /locus_tag="VHM48_14790"
     CDS             3478..4578
                     /locus_tag="VHM48_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756734.1"
                     /translation="MTFLILTGIHKRFGPTVAVEDFNLTAEKGEFVSFLGPSGCGKTT
                     TLRMIAGFEQPTAGTISIGGTDITNRPPNRRNIGMVFQSYALFPNMNVADNIGFGMKV
                     RKRPKDQIRKRVDELLGLMHLPDKGGRYPYQLSGGQQQRVALARALAVEPEVLLLDEP
                     LSALDAKIRVALRKEIRAIQRQFGITTVYVTHDQEEALSLSDRVVVMSEGRIEQIGAP
                     ADIYNFPATPFVASFVGTLSLLQANVIEGATARLSIDGQEIRGARPAAGDGQRVTVAL
                     RPEAVEQGDGGGSNRLRGTVEDVSFLGSIVRTRVRISGRNTLSFDQFNDPSLAASTIG
                     EEIAVSFSPESTLVLEGRASADAVIEEAIAEV"
     gene            4625..5362
                     /locus_tag="VHM48_14795"
     CDS             4625..5362
                     /locus_tag="VHM48_14795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2756735.1"
                     /translation="MTIALHPILDGIDLVVFDKDGTLIAFEAMWSGWARELGTRLEIV
                     TRRPVAGDVFTTIGFDPVSGRILAGGPLAIATMGQIQELVGAVLRRWCPSVNAARRVL
                     ADAWFEPDPVRSALPLADLDEVFTALRGAGRRIAIATTDDRRPTEATLAGLGLTGFVD
                     VVLCGDDHGPTKPEPAALAAIAAGLGIGIQRTAMVGDTPADLRMARGAGARPIGVASG
                     ITSAAELAPDADLVVDSIAMLVRSTRP"
     gene            5533..6714
                     /locus_tag="VHM48_14800"
     CDS             5533..6714
                     /locus_tag="VHM48_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2756736.1"
                     /translation="MPGTGKTFLRVAVLFAIVAIVASACSGTATPAPSGGPPGSPSSS
                     GSPASSIAPSAGSDDLAVLVAAAEKEGSLTTIALSHDWCNYGEAIKTFTTRYAIAINE
                     LNPDAGFGDQVSAIKANKDKGPQAPDVIDVGLSFGAKAKADGLLAPYKVGTWDSIPDA
                     AKDPDGFWYGDYYGVLAFETNKTKGATPPTDWADLLDPSHANQVALAGDPRVSSQAIQ
                     TVYAAALANGGSLDNAKPGLDFFAKLKKAGNLVTTIASPDTIDQGTTPVTIRWTYSAL
                     AHRDSANGSPDIDVTVPTSGRLAGMYVQAISAYAPHPNAARLWMEFLYSDEGQNIWLK
                     GYCNPIRFEDLASGNAIPADVLANLPDTAGAVFPTLDQLTTATTLITQKWNSVVGVDI
                     K"
     gene            6922..10983
                     /locus_tag="VHM48_14805"
     CDS             6922..10983
                     /locus_tag="VHM48_14805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2756737.1"
                     /translation="MAVAFEPPDHDALLSLGDVAAAAGITRGTARAWCLSGRLPSVAG
                     ARNEPLVRRSDLESWLARRGPDGNRMPVFRDPRAGGEALRRLASEVSGQIDLETLFGD
                     VVADAMDLFALARIGLWLYHADRRHPLSLAAQHGLADEVIGWVSTLAPDAPAAGLRAI
                     RTREVVTLGDTLIDTPTDDVRDLYARNGIRSVCFAPMIFRGDPVGLIVLYQDAVHAWS
                     ADETALARGLADQMAAAVGNARLNDSVRSLAGRLEAVQDLAIRLNRTRDFAEIADLII
                     EGTQRLIVHDSIRVYRVDHDSGWCEPIAFKGTFAGSTTPDPEMLRVQIGDGLTGWTAL
                     HNETLVVGNTNADPRALERLRSVGTESVLTVPISFEDHVHGVIVVAADGGDRFGPEDE
                     TTLTIFAGYAAQAIVNTTQLDQLDRQRRELEHQLASQRRLLEVNERLISTRDPKGVLE
                     MIADALKTIVPYDALTIYRCDFEAGVRRAVVARDRFADVILDYAGPISIGITGWVVAH
                     GEGVLANEALADPRSVQIPGTPSEPEAMIVVPVRVAGAVVGTLNVARMGGEEAYFSQN
                     EFELVQLFAGQASLALENAEAHGAITVRAEHDALTGLRNHGSFQLELGHATASAEGAP
                     FALLMLDLDSFKGFNDTCGHPAGDALLAGVADALRAATRSGDALYRYGGDEFAVILPG
                     ANRIEAFEVVERIRRGVASVPSPTGPRVTISAGVACYPDDGATKDELVSAADQSLYLA
                     KPTSRTDDPEAARRDPYLAALDETAIALMNRRDPEDLLETIISRAAALLGTPHGFIYL
                     LEPDGASLVIRHGIGVFTDFLGYRMPVDSGLSGLVHRESRPIAVDDYDAWMQRDSDLP
                     AQVFGAVVGVPLTSGGRGVGVIGLASGSYERTFGTREIGALSRFAQLASIALDNARLF
                     EAAQRGALYDPITGLPNRELLTDRVAHSLAWTRDDEGEPIALILLDLDRFKVINESLG
                     HAVGDSLLVAVGARLQHCLRPGDTVARFGGDEFGIILEGIGGVDEARRTADRILAELR
                     EPFELGNRDWYVNASLGIAMAWPGQATPGDLFREAEVALVRAKASPGPSYVLFEPEMS
                     AATLERVELENDLRRALDRNELRLHYQPLVDLATDRIVGLEALVRWEHPTRGLIPPLA
                     FIPVAEETGLIVPIGRWVLETACRQARRWLDEMPDSPLVMSVNLSARQFGQATLVDDI
                     RATLEATRLPADRLELEITESVLLAEGEESATALLAIRDLGVGLVLDDFGTGYSSLSY
                     LRRLPLDTIKIDRSFVAGIDEDNSNLPIVEAVIALAHALGMEVVAEGIETAGQLARLR
                     DLVCDRGQGYYYARPQPPEALTALLAGGTAPGV"
     gene            complement(11363..12067)
                     /locus_tag="VHM48_14810"
     CDS             complement(11363..12067)
                     /locus_tag="VHM48_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX2756738.1"
                     /translation="MLSNRYPGAAPSQVGVRPSTELAQRLLTHAFGWMFAGLLLTAGV
                     AVVVQTSPTLTNFATRNIFLLFIGQLALVFVISGAIQRLSATVALGLFFVYAATLGLT
                     LGLIVQFYTGASVSTAFLSASAMFGAAAVYGATTKRSLAGLGGILFMGLVGLIVASVL
                     NIFLHSSQMGWIISIVGVVLFTALTAYDVQRIQSGDVAAATGSLEKGAVLGALRLYLD
                     FINLFLFMLRLMGGRR"
     gene            complement(12067..12720)
                     /locus_tag="VHM48_14815"
     CDS             complement(12067..12720)
                     /locus_tag="VHM48_14815"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2756739.1"
                     /translation="MTDLDATPPFDSAVATRRGDDGTTGLLFGGERIAKDDLRTEAYG
                     TIDEAVAALGLARAQIGRAGDGAFALSVGELGAILLRIQRELFVAAAELATQPAARDR
                     QVDEVSRVTASMVSGLEAILSDAEADIVMPTEFVVPGETLVGAAIELARTIVRRAERR
                     AVTLGSEGLLPGPHLLPYLNRLADLLWVLARGAEQAEARSAPPSRPDRHRPSATRST"
     gene            complement(12748..13137)
                     /locus_tag="VHM48_14820"
     CDS             complement(12748..13137)
                     /locus_tag="VHM48_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX2756740.1"
                     /translation="MTDTTTDPTASPASAPAAGPEPQPEFFDASADATFDPQRRATEL
                     AILDALRAVVDPEIGMNVVELALIKQIILGPDTTEVKMILTTPFCPYAGSMIQQVKDQ
                     AESVVDHDVKVTLLAERWDPRDAGLMW"
     gene            complement(13193..13375)
                     /locus_tag="VHM48_14825"
     CDS             complement(13193..13375)
                     /locus_tag="VHM48_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756741.1"
                     /translation="MPDVGPLGFLLVLVYVLVVVAIIALPIFILREMRHRGTQLDRIE
                     RRLNDLDGRGGPPDPR"
     gene            complement(13388..13729)
                     /locus_tag="VHM48_14830"
     CDS             complement(13388..13729)
                     /locus_tag="VHM48_14830"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2756742.1"
                     /translation="MTIKWVQVDPLVMNGEPFCYGSRLTVRQLLELRSNGFDLPRLIK
                     DHPELKRVGIAAAYTYAADHHERYADFFEADGSLAGPGYDEAEAAGLPEQYRRPGIVI
                     KPGAAATGSVG"
     gene            complement(13733..13921)
                     /locus_tag="VHM48_14835"
     CDS             complement(13733..13921)
                     /locus_tag="VHM48_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756743.1"
                     /translation="MNDLILLCESIRAAIGATLAAADMGPVDAAIEAMIERFKWHGRI
                     AGDGRKKSRLLQAIYKGA"
     gene            14131..>14374
                     /locus_tag="VHM48_14840"
     CDS             14131..>14374
                     /locus_tag="VHM48_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756744.1"
                     /translation="MSHPSLGLPPIDNNSALPPAAARVDAARGRLAARALEIAIDRDP
                     TLRERHDEYAVRRLLRDATVLIDRIVAAIAADDPGPA"
BASE COUNT         2182 a         5204 c         4817 g         2171 t
ORIGIN      
        1 ccgcattcga ggtcgagcag gaatgccgtg agctcgtcta cgccgcccgg ttcctgccgc
       61 gctggcggta cgcgcccctg gccacgctcc gtgcccggta cgggacgtga acagccgcca
      121 tgggaccatc gcggcgatga acccgctcca attcctcgcc gacctcgaga ccaggccgca
      181 gtggctgggc ttgctcgccg atcggttcgc ccggggcaat ccgttcacgg ccgtgccgcg
      241 cgacgtcgag cgcggccgag gtcctcgtcg cggagctgct cgccgcgacc tggtggcgcc
      301 aaaccgcgcc cacgcgtgca tgggtcggca acaagccggt gacgaatcga tcacactctt
      361 gacgcagcgt gccgccgagc agtgaaatgc tcgcgactgg cacagggccc gctccggcat
      421 catcggatcg ggtcgccgtc acgacccgcg cgatcgccgt aggaggagat ctgatcgtgc
      481 cgcgcccggc gacgagcagc tcgccagcat cgtcccggat ccccgtcatc cgtccagagg
      541 agatctgacc ttgaccacga ggaagcccac cattcggatc gccgcggcgt tcgcggtctt
      601 cgcgatggtt ctctcagcct gcagcagcgg cactggcggc gccagcgccg gaaccgcggc
      661 aacgatcccc ggagtccccc agaccctgat cgacgccgcc aagaccgaag gcaacctgac
      721 cacgatcgcc ctgcctcatt cgtggtgcga ctacggcgac cttctgagcc agttcacgag
      781 caagttcggc atcaagatca acgagctgaa tccgaagggc gggtccaagg acgagatcac
      841 cgccctcgag gcaaacaaga cgaacaaggg cccggccgcg ccggacgtcg tggacgtggg
      901 cctcgcgtac gggccgcagg ggaaggcggc gggcgacttc cagacctaca aggtcgcgaa
      961 gtgggacacg atcccggcct cggtcaagga tgccgacggc gcgtggtatg gcgactactt
     1021 cggtgtcctc gcgttcgagg tcaacaccgc caaggtcaag aatgtcccgc tggactggag
     1081 cgacctgctc aagccggagt acaaaggcca ggttgccctc gccggtgacc cgaccgtctc
     1141 caaccaggcg atgtccggcg tgtgggccgc ggcgctcgcc agcggtggct cgctcgatga
     1201 cgcgacgccg ggcctggcct tcttcaagaa gctcaatgat gccggcaact tcgtgccgat
     1261 catcgccgag accgccaacg tcgcctccgg cgacgctgcg atccggatcg catggaccta
     1321 caacgggctt gccgaccgcg actcgctgaa gggcaatccc ccgatcaccg ttgtcgtccc
     1381 gaagagcgga cgcttcggcg gcgtgtatgt ccaggccatc agcgcgttcg ccccgcatcc
     1441 gaacgcggcc aagctctgga tggagtggtt gtactccgac gcgggccaga atctctacct
     1501 caaggggtac tgcaacccga tccggtacga cgacatggtc aagaacaaga ccgtcgacgc
     1561 aacagcccag gccgcgctgc ccgacgccac cggggccgtg ctgccgaccc tcgaccagat
     1621 cacgaaggcg accgccatcg tcgccaaggg ctggccgacc accgtcaacg tcgtcgtcaa
     1681 gtagccccct ctcgagcctg aagagcggag cagcgcaatg gccactgcgc caccgctcaa
     1741 cctccggggc gcgccaccgg cgcgccccgg aggtcgtctt tcgtggtcgt ggctcggggt
     1801 gttgccgttc ttcctgttcg tcctgctctt cctcgtcata ccgacgctct atctcctggt
     1861 cggcagcttc gaggacaagg cgggcagcct gaccctccag aactacgccc acctgagcga
     1921 accgaacctc gctgattcct acttcgcgag catcgagatc agcctcgtca ccgcgatcgc
     1981 cggcggcatc ttcgggttcc tcgttgccta cgcggtgatc ctcggcggac tgccgtcgtt
     2041 cctgaggacc gcgatcatga ccttctccgg ggtggcctcg aacttcgccg gggtgccgct
     2101 ggccttcgcc ttcacgttca ccctcggcgc atcgggcatc ctgacgcccc tgatcgaggc
     2161 cctcggggtc cacttcaaca tctattcgaa gacggctctc gagctcgcct acttctactt
     2221 ccagttcccg ctgatgatcc tgatcatcgc gccggccatc gacgggctgc gcaaggactg
     2281 gcgtgaggcg gccgagaaca tgggagcgag ctcgttccaa tactggcgcc atgtcgccct
     2341 gccgatcctc acgccgtccc tcctcggcac cgtgattctc ctgttcggga acgcgttcgg
     2401 cgcccaggcg accgcgttcg cgttgagcgg cggctcgatc aacatcgtga cgatcgcgat
     2461 cacgcgccag atccgcggcg acgtcgccaa tgatcccggc gtcggctacg ccctcgcgat
     2521 gggcatggtc gtgatcatgg ccatctcgat cgggatctat agctggctgc agcgacgctc
     2581 ggagcggtgg ctccgatgac gggcggcacc ccgctcgtcg cgcaggcccc gaaaggcccg
     2641 gattccctcg cggccgcccc ggtctccagg ccacgccgca gcttcgggcg gatctggtgg
     2701 tgggccgtct tcgcggtcgc ggtgctctac ttcgcgctgc ccctcatcgg gacgttcgcg
     2761 ttctcgatgc gctcccagcc cttgttctcc gcctacgcgg tcgtgaccga cgatccgcag
     2821 ttcgcggcga ccctgctcta ctcgttcgtg gtcgcgattg tgacgatcat cgtgagcatc
     2881 gtcctgatcg tcccgaccgc cttctgggtc cggctgcgcc tgccgcgcct gcggccgtac
     2941 atcgagttca tcacgctcct gccattcgtg atcccgccga tcatcctcgt cttcggcctg
     3001 ctccggtcgt acagcggcga cccgctgccg ctcgccggct cggacttcgg aagcgacgtc
     3061 ctgctcgtgg ccgcgtatgt cgtgctatcg ctcccataca tgtaccgggc agtcgatacc
     3121 gggctccgag cgatcgacgt ccgaagcctg acggaggcgt cccagagcct tggcgcgagc
     3181 tggttcacga tcctcgtccg ggtcatcttc ccgaacctgc gcgtgtcgat cttgagtggg
     3241 gccttcctga ccctcgccat cgtcgtcggt gagttcacca tcgcgacgtt tctggcccgt
     3301 ccggccttcg gtccgtacat ccagctcctc ggcggcaaca aggcatacga gccggccgcc
     3361 gtttcgctca tcagcttcgg gctgacgtgg atcgcgatgg gcctgctcgc gctcatcgga
     3421 cgccagtcgc gccagaagat ccagatcgcc ggcgcgcatt gacggagcga tgacctcatg
     3481 accttcctca tcctcaccgg catccacaag cggttcgggc cgacggtcgc ggtcgaggat
     3541 ttcaacctca ccgccgagaa gggcgagttc gtgtcgttcc tcgggccgtc gggctgcggc
     3601 aagacgacga ccctccggat gatcgcgggg ttcgagcagc cgacggccgg cacgatctcg
     3661 atcggcggga ccgacatcac caatcggccg ccgaaccggc ggaacatcgg catggtcttc
     3721 cagtcgtacg ccctgttccc gaacatgaac gtcgccgaca acatcggctt cggcatgaag
     3781 gtccgcaagc gaccgaagga ccagatccgc aagcgcgtcg acgagctgct cgggctcatg
     3841 cacctgccgg acaagggcgg tcgctatccc taccagctgt cgggcgggca gcagcagcgg
     3901 gtcgcgctgg cccgggcgct cgcggtcgag ccggaggtgc tcctcctcga cgagccgctg
     3961 tccgccctgg acgccaagat ccgggtcgct ctccgcaagg agatccgggc catccagcgc
     4021 cagttcggga tcaccaccgt ctacgtcacc cacgaccagg aggaagcgct gtcgctgtcc
     4081 gaccgggtcg tcgtgatgag cgagggacgg atcgagcaga tcggcgcacc cgcggacatc
     4141 tacaacttcc cggccacgcc gttcgtggcg tcgttcgtcg ggacgctcag cctgctccag
     4201 gcgaacgtca tcgaaggggc gacagcccgc ctgagcatcg acggccagga gatccgcggc
     4261 gcgcggcccg cggcgggcga cggccagcgg gtcaccgtcg cgctccgccc ggaggcggtc
     4321 gagcagggcg atggcggcgg cagcaaccgc ctccgcggca ccgtcgagga cgtcagcttc
     4381 ctcggttcga tcgtccggac gcgcgtccgc atcagcggcc gcaacaccct gtccttcgac
     4441 cagttcaatg acccgagcct cgccgcgtcg acgatcggcg aggagatcgc cgtctcgttc
     4501 tcgccggagt cgaccctcgt cctcgaaggc cgggcgagcg ccgacgccgt catcgaggag
     4561 gcgatcgcgg aggtctgagg gccgtcgatc gcggcccgac gacagcctga gccctcgtcc
     4621 cgagatgacg atcgccctcc atccgatcct cgacgggatc gatctcgtcg tcttcgacaa
     4681 ggacgggacc ctgatcgcgt tcgaggcgat gtggagcggg tgggcccgcg agctcgggac
     4741 gcggctggag atcgtcacgc gccggccggt cgccggtgac gtcttcacga cgatcggctt
     4801 cgacccggtg tcgggtcgga tcctcgccgg cggtccgctc gcgatcgcga ccatgggcca
     4861 gatccaggag ctcgtcgggg ccgtgctccg gcgctggtgc ccgagcgtga acgcggcccg
     4921 tcgggtcctc gccgacgcct ggttcgaacc ggatccggtg cgatccgccc tgcccctggc
     4981 cgacctggac gaggtgttca ccgcgctccg gggcgccggc cgcaggatcg cgatcgcgac
     5041 gaccgacgat cggcgaccga ccgaggcgac cctcgcgggc ctgggcctga ccggcttcgt
     5101 cgatgtggtc ctgtgcggcg atgaccacgg tccgaccaag ccggagccgg cggcgctggc
     5161 cgcgatcgcc gccgggctcg ggatcgggat ccagcggacg gcgatggtcg gcgacacgcc
     5221 ggccgacctt cggatggcac gcggcgcggg agctcgcccg atcggcgttg cgagcggcat
     5281 cacgtcggcc gccgagctcg ccccggacgc ggatctcgtc gtcgactcga tcgcgatgct
     5341 ggtccgttcg acgcggccct gacgcgacgg cgggccgtcc gcgcagagcc tgatgcggcc
     5401 tggatcgccg ccggacccgt tcgccacgac ccccgcgatc gccgtacgat gggatcggat
     5461 cacgacggac cgcgatcggg tcacggcggc ggcgtcgcgt ccgcaacccg ctcactcgac
     5521 cctggagatc ggatgcccgg caccggcaag accttcctcc gcgtcgccgt cctgttcgcg
     5581 atcgtcgcga tcgtcgcctc ggcctgcagc ggcaccgcca cgcccgcgcc gtcgggcggg
     5641 ccgccgggtt cgccgtcgtc ctcagggagc cccgccagct cgatcgcacc gtccgccggg
     5701 tcggacgacc tggccgtcct cgtcgccgcg gccgagaagg aaggcagcct cacgacgatc
     5761 gcgctgtccc atgactggtg caactacggc gaggccatca agaccttcac gacgcgatac
     5821 gcgatcgcga tcaacgagct caatccggat gccggcttcg gcgaccaggt cagcgcgatc
     5881 aaggcaaaca aggacaaggg tccgcaggct ccggacgtga tcgatgtcgg cctgtcgttc
     5941 ggcgcgaagg cgaaggccga tgggctgctc gccccgtaca aggtcgggac ctgggacagc
     6001 atccccgacg ccgccaagga tcccgacgga ttctggtacg gcgactacta cggcgtcctc
     6061 gcgttcgaga cgaacaagac gaagggcgcc acccccccga ccgattgggc cgacctcctc
     6121 gacccgtcgc acgcgaacca ggtcgccctc gccggcgacc cgcgcgtatc cagccaggcg
     6181 atccagacgg tctacgccgc ggcgctggcc aacggcggct cgctcgacaa tgccaagccc
     6241 ggactggact tcttcgccaa gctcaagaag gccggcaatc tcgtgacgac gattgcatcg
     6301 ccggacacga tcgaccaggg gacgacgccg gtcacgatcc gctggacgta cagcgccctc
     6361 gcccaccgcg acagcgccaa cggcagcccc gacatcgacg tcacggtccc gacgtccggg
     6421 cggctcgcgg ggatgtacgt ccaggcgatc agcgcctacg ccccgcatcc gaacgcggcc
     6481 aggctgtgga tggaattcct gtacagcgac gaaggccaga acatctggct gaagggctac
     6541 tgcaacccga tccggttcga ggacctggcg tccggcaatg cgatcccggc cgacgtcctg
     6601 gccaacctcc cggatacggc cggcgccgtc ttcccgacgc tcgaccagct cacgaccgcc
     6661 acgaccctga tcacccagaa gtggaattcg gtggtcggcg tcgacatcaa gtagccgacc
     6721 gtcgccaggc cgcggccgcg ggcagcgcga ccgtggcgcc gggcgcgaca gcggcccctc
     6781 gtcaatggca ccccccggat cgggcggtac ctctgggcca tcgctccggc acggaccggc
     6841 ccgtaccgtt ggcacgtcac cgcccgggcg gccctgttcc ccgcgcccgt tcagtccaga
     6901 tcgaggtcac cgccgccgca gatggccgtc gcattcgagc caccggatca cgacgcgctc
     6961 ctgagcctcg gcgacgtcgc cgccgccgcc gggatcaccc gtgggaccgc tcgcgcctgg
     7021 tgcctgagcg gccgcctgcc gtcggtcgcc ggcgcgcgca acgagccgct cgtccgacga
     7081 tccgacctcg aatcgtggct cgcccgccgc ggaccggacg gcaatcggat gccggtcttc
     7141 cgcgatcccc gggccggtgg ggaggccctt cgtcgactcg cgtcggaggt cagcggccag
     7201 atcgatctcg agaccctgtt cggggacgtc gtcgccgatg cgatggacct cttcgccctc
     7261 gcccggatcg gtctctggct gtatcacgct gaccgtcgtc atccgctcag cctcgccgcc
     7321 cagcacgggc tcgcggacga ggtcatcggc tgggtgtcca cgctcgcccc ggacgccccg
     7381 gcggccggcc tccgtgcgat ccggacccgc gaggtcgtga cgctcggcga caccctgatc
     7441 gataccccga ccgacgacgt tcgcgacctc tacgcgagga acgggatcag gagcgtgtgc
     7501 ttcgctccga tgatcttccg cggcgatccg gtcgggctca tcgtcctcta ccaggacgcg
     7561 gtccacgcct ggtccgccga cgagacggcg ctcgcccgtg gcctggccga ccagatggcg
     7621 gcggccgtcg gcaacgcccg gctcaatgac tccgtccgct cgctcgcggg gcgcctcgag
     7681 gccgtccagg acctcgccat ccggctgaac cggacccgcg acttcgccga gatcgccgat
     7741 ctcatcatcg agggcaccca gcgactcatc gtccacgact cgatccgggt ctatcgggtc
     7801 gaccacgaca gcggctggtg cgagcccatc gcgttcaagg gcacgttcgc gggctcgacc
     7861 acgccggacc cggagatgct ccgcgttcag atcggcgacg gcctgaccgg ctggaccgcg
     7921 ctccacaacg agaccctcgt ggtcggcaat acgaacgcgg atccgcgagc cctcgagcgg
     7981 ctccggtcgg tcggcaccga atcggtcctg accgtcccga tcagcttcga ggaccacgtc
     8041 cacggcgtca tcgtcgtggc ggccgacggc ggcgaccgtt tcggcccgga agacgagacg
     8101 acgctcacga tctttgccgg ctacgccgcc caggcgatcg tcaacacgac ccagctcgat
     8161 cagctcgacc gccagcgacg cgagctcgag caccagctcg ccagccagcg tcggcttctc
     8221 gaggtcaacg aacggctgat ctcgacgcgc gacccgaagg gcgtcctcga gatgatcgcg
     8281 gacgccctca agacgatcgt gccgtacgac gcgctgacga tctaccgctg cgacttcgag
     8341 gccggcgtcc gccgtgcggt cgttgcccgc gaccgcttcg ccgacgtgat cctcgactat
     8401 gccgggccga tcagtatcgg gatcaccggc tgggtggtgg cccacggcga gggtgtgctc
     8461 gccaacgaag ccctcgccga tccgcgctcg gtccagatcc ccggcacgcc atccgagccg
     8521 gaggcgatga tcgtcgtccc ggtccgtgtc gcgggggcgg tcgtcgggac gctcaacgtg
     8581 gcccggatgg gcggcgagga ggcgtatttc agccagaacg agttcgagct cgtccagctg
     8641 ttcgccggcc aggcgtcgct ggctctcgag aatgccgagg cccacggcgc gatcaccgtt
     8701 cgggccgagc acgacgcgct caccggcctc cgcaatcacg gctcgttcca actcgagctc
     8761 ggccacgcca ccgcgtcggc cgagggggcg ccgttcgcgc tcctgatgct cgacctcgac
     8821 tcgttcaagg gcttcaacga cacctgcggt catccggccg gcgacgccct cctcgccggc
     8881 gtcgccgatg cgctgcgggc cgcgacgcgg tccggcgatg cgctctatcg ctacggcggt
     8941 gacgagttcg ccgtgatcct gccgggggcc aaccggatcg aggccttcga ggtcgtcgag
     9001 cggatccggc gcggcgtcgc gagcgtgccc tcaccgaccg ggccgcgggt cacgatctcg
     9061 gccggcgtgg cctgctaccc ggacgacggc gcaacgaagg atgagctcgt ctccgcggcc
     9121 gaccagtcgc tgtatctcgc aaagccgacg tcgcgaaccg atgatcccga ggcggcccgc
     9181 cgcgatccgt acctggcggc cctcgacgag acggcgatcg cgctgatgaa ccggcgcgac
     9241 ccggaggacc tgctcgagac gatcatcagt cgcgccgccg ccctgctcgg cacgccgcac
     9301 ggcttcatct acctcctcga gccggacggc gcctccctcg tcatccgcca cggcatcggt
     9361 gtcttcaccg acttcctcgg ctaccggatg ccggtcgaca gcgggctgtc gggcctcgtc
     9421 caccgtgaga gccggccgat cgccgtcgac gactatgacg cgtggatgca gcgcgacagc
     9481 gacctgccgg cccaggtgtt cggtgccgtc gtcggcgtcc cgctgacgtc gggcggccgt
     9541 ggcgtgggcg tgatcgggct ggcgtcgggg agctacgagc ggacgttcgg gacgcgcgag
     9601 atcggcgccc tcagccggtt cgcccagctc gcctcgatcg ccctcgacaa tgcccgcctg
     9661 ttcgaggcgg cccagcgggg ggcgctctat gacccgatca ccgggctccc gaaccgcgag
     9721 ctcctgaccg accgggtcgc ccactcgctc gcctggacgc gcgacgacga gggcgagccg
     9781 atcgccctga tcctgctcga tctcgaccgg ttcaaggtga tcaacgagag cctcgggcac
     9841 gcggtcggcg attcgctgct ggtcgcggtc ggcgcgcggc tccagcactg cctccgaccc
     9901 ggcgacaccg tcgcccgctt cggtggcgac gagttcggga tcatcctcga ggggatcggc
     9961 ggggtcgacg aggcccgccg gacggccgac cggatcctcg ccgagctgcg cgagccgttc
    10021 gagctcggca accgcgactg gtacgtcaac gccagcctcg ggatcgcgat ggcctggccg
    10081 gggcaggcga cgcccggcga cctgttccgc gaagccgagg tcgcgctcgt ccgggccaag
    10141 gcctcgccgg gtccgagcta cgtcctgttc gagccggaga tgagcgcggc gaccctcgag
    10201 cgggtcgagc tcgagaacga cctccgccgg gcgctcgacc ggaacgagct gcgcctccac
    10261 taccagccgc tcgtcgacct cgcgaccgac cggatcgtcg ggctggaggc gctggtccgc
    10321 tgggagcacc cgacccgcgg cctgatcccg ccgctcgcgt tcatcccggt cgccgaggaa
    10381 accggactga tcgtcccgat cggacgctgg gtcctcgaga cggcctgccg ccaggcgcgt
    10441 cgctggctcg acgagatgcc cgactcgccg ctggtgatga gcgtcaacct gtcggcccgc
    10501 cagttcggtc aggcgacgct cgtcgacgac atccgggcga cgctcgaggc gacgcgcctc
    10561 ccggccgacc ggctggagct cgaaatcacg gagtccgtcc tgctcgccga gggcgaggaa
    10621 agcgcgacgg ccctcctggc gatccgcgac ctcggggtcg ggctcgtcct cgacgacttc
    10681 gggaccggct attcgtcgct gtcgtatctg cgccgtctgc cgctcgacac gatcaagatc
    10741 gaccgctcgt tcgtcgccgg catcgacgag gacaactcga acctgccgat cgtcgaagcc
    10801 gtgatcgccc tggctcacgc gctcggcatg gaggtcgtgg ccgagggcat cgagacggcc
    10861 ggccagctgg cccgcctgcg cgacctcgtg tgcgatcgcg gccagggcta ctactacgcc
    10921 cgcccgcagc caccggaggc gctcacggcg ctgctcgccg gcgggaccgc gccgggcgtc
    10981 tgacgaggcg cgcgcggccg ggagcagcac cggccgccgc gggcactgac ccggcggcgt
    11041 cccatcgaat acggcacggc accaaacctg gcgaatattc acccacggac tcttgggcca
    11101 ggtttgggcg gccaaaccgc ttgaatcgag tccatcgtta gtcgtcgaag gctaactcga
    11161 ccgcctcacc cggtgcggat cgtgccgaac agtcccccgg cgaatatccg gccaggtatc
    11221 ggtcgccggt gtcgttgaag cagctggtgc acctctcgcg acccgtctgc ggaaccgatc
    11281 gtttccccgg ccccaacgcc cgagccgaaa tcgtgatggc cggacgccgc cgcgcaatgc
    11341 cctcgtgcgc ggccaaggat ccctagcggc gcccgcccat gagccggagc atgaacagga
    11401 acaggttgat gaagtcgaga tacaggcgga gcgcgccgag gaccgctccc ttctcgagcg
    11461 aaccggtcgc ggcggcgacg tcgccggatt ggatccgctg cacgtcgtag gcggtcagag
    11521 cggtgaacag cacgacgcca acgatcgaga tgatccagcc catctggctg ctgtgcagga
    11581 agatgttcag cacgctcgca acgatcagcc cgaccaggcc catgaagagg atcccaccga
    11641 ggcccgcgag ggaccgcttc gtcgtcgcgc cgtagaccgc cgccgcaccg aacatcgccg
    11701 acgcgctcag gaacgcggtg ctgaccgagg ctccggtata gaactggacg atcaggccga
    11761 gagtcagccc gagggtcgcg gcatagacga agaagaggcc gagcgcaacg gtcgcgctga
    11821 gccgctggat cgcaccgctg atgacgaaga cgagggccag ctggccgatg aacagcagga
    11881 agatgttgcg ggtcgcgaag ttcgtcaggg tcggcgacgt ttggaccacg accgccacgc
    11941 cggcggtcaa caggaggccc gcgaacatcc agccgaaggc atgggtcagc aggcgctggg
    12001 ccagctcggt ggatggccgg acgccgacct gcgaaggggc cgcgccggga tatcgattgc
    12061 tcagcatcag gtgctcctcg ttgcggaagg tcggtgtcga tccggacgtg atggcggcgc
    12121 cgaccgcgcc tccgcctgct cggcccctcg cgcgagaacc cagagcaggt cggcaagccg
    12181 gttcaggtac ggtagcaaat ggggacccgg aagcaatccc tccgaaccga gcgtcaccgc
    12241 cctgcgttcg gctcgccgga cgatcgtccg ggccagctcg attgcggcgc cgacaagggt
    12301 ctcacccgga acgacgaact cggtcggcat gacgatgtcg gcctcggcgt cgctgaggat
    12361 cgcctcgagg ccgctgacca tcgaggccgt cacccggctg acctcgtcga cctggcgatc
    12421 ccgggcggcc ggctgggtcg cgagctcggc tgccgccacg aacagctcgc gctggatccg
    12481 caggaggatc gcgccgagct cgccgaccga cagcgcgaag gcaccgtcgc ccgcgcgtcc
    12541 gatctgggcg cgggcaagcc cgagcgcggc gacggcttcg tcgatcgtgc cgtaggcctc
    12601 cgtccggagg tcgtccttgg cgatccgctc gccaccgaac aggagcccgg tcgtgccgtc
    12661 gtcaccgcgc cgcgtcgcga cggcggagtc gaagggtggg gtggcgtcga ggtcggtcac
    12721 cgggcgatca gccgaggagc ggctcgctca ccacatcagg ccggcgtcgc gcgggtccca
    12781 gcgctcggcc aggagggtca ccttgacgtc gtggtcgacg accgattcgg cctggtcctt
    12841 gacctgctgg atcatcgatc ccgcgtacgg gcagaacggc gtcgtgagga tcatcttcac
    12901 ctcggtcgta tcggggccga ggatgatctg cttgatcagg gccagctcga cgacgttcat
    12961 cccgatctcc gggtcgacga ccgcccggag ggcatcgagg atcgcgagct cggtcgcccg
    13021 gcgctgcggg tcgaaggtgg cgtcggccga ggcgtcgaag aactcgggct gcggctcggg
    13081 gccggcggcc ggggccgaag ccggggaagc ggtggggtcg gtcgtcgtgt cggtcatcgg
    13141 cgcgatccta ccaccgaccc tcgaacagcc cgatcggagg cgttcggcgg cgtcagcgcg
    13201 gatcgggcgg gccgccccgg ccgtcgagat cgttcaggcg ccgctcgatc cggtcgagct
    13261 gcgttccgcg gtggcgcatt tcgcggagga tgaagatcgg cagggcgatg atcgcgacga
    13321 cgacgaggac gtagacgagg acgaggagga acccgagcgg gccgacgtcc ggcattgggc
    13381 cggccggcta cccgacgctg ccggtcgccg cggctccggg cttgatcacg atccccggcc
    13441 ggcggtactg ctcgggcagc ccggccgcct cggcttcatc gtagccgggt cccgccagcg
    13501 agccgtcggc ctcgaagaag tcggcatagc gctcgtggtg gtcggcggcg taggtgtacg
    13561 ccgcggcgat cccgacccgt ttgagctccg ggtggtcctt gatcaggcgc ggcaggtcga
    13621 acccgttgct gcgcagctcg agcagctggc ggacggtcag ccgtgagccg tagcagaacg
    13681 gctcgccgtt catgacgagc gggtcgacct ggacccattt gatcgtcatc gctcaggcgc
    13741 ccttgtagat cgcctggagc aggcggctct tcttgcgccc gtcgccggca atccggccgt
    13801 gccatttgaa gcgctcgatc atcgcctcga tcgcggcgtc gaccgggccc atatcggcgg
    13861 ccgccagcgt cgccccgatc gccgcccgga tgctctcgca gagcaggatc aggtcgttca
    13921 tcgggacctt gcggcggcga tacgccggcg gcgggcgaag gcgtcccgta gcgatcgctc
    13981 ggcggtcgtt cgaggagggg ccgccgatcg cccggttcgt tggcgggggt cgtcacgcgg
    14041 ccatcgtatc gactcgcgtg ggcgcgggac ccggactccg cgcgcccgcc cgtccgcaca
    14101 ccggctcggc cgcgcgctag actgccgatc gtgagccatc cctccctcgg cctgccgccg
    14161 atcgacaaca actcggccct gcccccggcc gccgcccgcg tcgacgccgc ccgcgggcgc
    14221 ctcgctgcgc gcgccctcga gatcgcgatc gatcgcgacc cgacgctccg cgagcgccac
    14281 gacgaatacg cggtgcgccg cctgctacgc gacgcgaccg tcctgatcga ccggatcgtc
    14341 gcggcgatcg cggccgacga ccccgggccg gcac
//
LOCUS       DATAIW010000502         8260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_258320, whole genome shotgun sequence.
ACCESSION   DATAIW010000502 DATAIW010000000
VERSION     DATAIW010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258320"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1232)
                     /locus_tag="VHM48_14845"
     CDS             complement(231..1232)
                     /locus_tag="VHM48_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756745.1"
                     /translation="MSAPSRTSTAAGGGSASARPGAPRDPDLILARVHLLLGSLSLAR
                     TELETLAGRGVLDDEAIRDLAEARWRTGDVLGAGEAATEWLVAHPDDVLGLVIAAEAQ
                     ATLGRPGEARRLAGRAMERADGSLDPVFAGMQRSPIWPVEPGSSVGPVGVLFDDLHPG
                     PQAVDPLPPPLDGRSGGFGTFPDDDPDALPGSAVPIAPATGPSLWGDEAAGRAVTSAD
                     ALDPTTLFHQARVALEAGQAAEAATGLILALRASPGLAPAVLDLLAGRSDSILALVRG
                     DAQRIVGREVEAMRDHAAAAEGLAAGDTTDDESGSARPVAADADVDTDPTDPAQEDS"
     gene            complement(1229..2500)
                     /locus_tag="VHM48_14850"
     CDS             complement(1229..2500)
                     /locus_tag="VHM48_14850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2756746.1"
                     /translation="MFERTALAGGPRVISARLPGARSVSIAAYVLAGSRLETPEEAGV
                     AHFMEHITFKGTERFPTTRAISEAIEGVGGSFNAATDRESTVYWVRVPRREATRAMDV
                     LGELIVRPKLDEAEIDGERTVIVEEIRSYLDDPAEYAQTLFQQAMFGSGSLGREICGD
                     EDGIRALPSDTIRSFWRTMYRPANTVIAVAGDVDHREAVDLAAAAFGTGNGAIPGYAA
                     APQLPAGERALLRARRDTTQAQLIVGVPALRRDHPDAWILAVLNGVLGDGMSSRLFLS
                     VREAQGLAYDVSSGLVDYADAGSLEVSAGVDPERLPAALDAVLAELARLRDEPVPADE
                     LAKTKAYLSGGLELRMDETRHLASWIGGQEALHDRVLDLDEALAAVAAVDATEVQRLA
                     RELFRDEGLRLAVVAPARHLRGLGRRLRLPA"
     gene            3085..4356
                     /locus_tag="VHM48_14855"
     CDS             3085..4356
                     /locus_tag="VHM48_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2756747.1"
                     /translation="MASTRLRLVSLLAATAIVVGACSSTPKASGPAASGGVANDCAAG
                     SAKGGGTGTLQVPEVVSGKFNVAMVLIGPHDDGGWSQAHYEGLQYLCQHLADSHVAYI
                     ELVPEGTDSEQVFRSLARKGFNFIIGTSFGYMDPMETVAKDFPDATFLHLTGYKSNGK
                     NFGNFFGAVEDFKYLAGMLAGSRAHKDGIPKIGYMATFPIPEEIRLGNAIARGVAVTC
                     PECKIDVRFINTWHDPQKERDGAASLFDAGADVVFTGADTPAVADVAAEKGKWAVTYD
                     HPSSCNKDSCLTAPYWIWGPEYTRIGQQVKDKKYTAGYEYFDANSKAMGLFGFMDGQT
                     PQKGVADLPPADVTAVKDLLAKMLAGTSDRFEVFAGPLKDNTGKVLLKAGEKLKQSDL
                     DQFPPGAPGSECTVCMYWWNENITVPLPKLS"
     gene            4421..5995
                     /locus_tag="VHM48_14860"
     CDS             4421..5995
                     /locus_tag="VHM48_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756748.1"
                     /translation="MTEEPAARPLAVEARGIVKTFPGVRANDQVDFDLRRGEVHALLG
                     ENGAGKSTLMNVLAGLYRQDAGEILVDGAPVAFGSPRDAIAAGIGMVHQHFTLVPSQT
                     VTENVLLGLDRPRFFLRERQFEGEVAALATRFGLHVDPRAKIWQLSVGEQQRVEILKL
                     LYRGARILIMDEPTAVLAPQEADELFVTLRSMTAEGRSVVFISHKLGEVLAIADRVTV
                     MRRGRVTAAGLPTAGATKADLARLMVGRSVIDAPDRTPFAPGAVVLAVHGVSALNDRG
                     LPAMRDVSLDVRSGEIVGIAAVAGNGQSELAEVVTGLRPCQGEILVDGDSVANRPASV
                     VLRHGVAHVPEDRTTVGTAPNLSLTDNLIMKRYRDAPVAHGLLVDEAAARAFAGGLRD
                     TYRIASPNVDTPARMLSGGNLQRAILAREIETNPGLLIAVQPTRGLDVGAIESVHRLL
                     LDLRAGGGAILLISEELDEILALADRVDVMYEGRVVGSMPVEEAEIGRIGLLMTGGSE
                     ADRDATPVGAAPGVAR"
     gene            5992..7143
                     /locus_tag="VHM48_14865"
     CDS             5992..7143
                     /locus_tag="VHM48_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756749.1"
                     /translation="MRIHLEPRTTTPRWLSPLATLGAVAVALVISGAVIGLVGGDPVR
                     SYLHIVEASFGSIGVLSDTLVKATPLILTGLACALAFRMRLWNIGAEGQFLLGAWGAS
                     AVVLFPLVPSGTPAIILIPLMMLAGMLAGGLWGLIPGALRALLGVNEIIVTLMFNYVA
                     LFWVQFWVFGPWSEGGFQQTHPFPPQAWLPRLTDFSSVVPGFAGLTVHAGLLFGLVAA
                     GLLWLFLERSRFGYEIRLIGDSPRAAEYAGINIKRRLIIVFAISGALAGLAGMSEVNG
                     AVHRLQDHISPGYGFTAVIVAYLAKFRPGRVIVAAILFGALILAGREIQPSGVPVMIQ
                     GIILFTLIVGDVLVRYRIRITRGGPAPAGPLTPATGSSIGPAPRPEARG"
     gene            7143..8108
                     /locus_tag="VHM48_14870"
     CDS             7143..8108
                     /locus_tag="VHM48_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756750.1"
                     /translation="MDLGLIAAAGVATGTVLLFAAIGEIFAERAGVMNLGVEGMMLMG
                     AVAGFSVTGATGNPWIGVMAAMLVGGLFGLLHGIVTIHLRADQVVSGLALTFLGTGLA
                     RVLGEGLTNTNVERLPAFTIPVLANLPIVGPIAFQNQSILVYVGFLLVPLTWFWIART
                     RPGLHLRAVGESPAAADVQGIGVYRLRLVYVVFGGILAGLAGATITLAVAPGWFGDQT
                     VNSKGWIAIGLVIFARWSPIWAAFGAYLFAAISPFILALQGVPTILGVENPFLPGRSA
                     TFFLEMLPYLFVVGVVILGSRSAARRRIGAPAALGLPYVRGERGL"
BASE COUNT         1165 a         2949 c         2894 g         1252 t
ORIGIN      
        1 gagcgggacg aacctcgtcg tggatgacat cgtcttcacc aagggatagg gggtcggctc
       61 acgaggcgat acccgggact gacttgacac tggcgcttgc cgctgttgtc gtcccgggtc
      121 cgggtctgga tccggccctc gatcgcgacc tccaggccct tgcccaggaa ctggccgcgg
      181 gtggccgcag cctcagcgtt tcgatgagta ctccgctgga ataccttcgt tcacgaatcc
      241 tcctgggcag ggtcggtggg atcagtgtcg acatccgcgt cggccgcgac cggccgcgcg
      301 gatccggatt cgtcgtcggt cgtgtcaccg gccgcgagac cctcggccgc cgcggcatgg
      361 tcgcgcatcg cctcgacctc ccgaccgacg atccgctggg cgtcgccgcg gacgagggcg
      421 aggatcgaat cgcttcggcc ggcgaggagg tcgaggaccg ccggcgccag cccgggcgag
      481 gcacgcagcg ccaggatcag tccggtcgca gcctccgcgg cctgaccggc ctcgagggcg
      541 acccgcgcct ggtggaacag ggtcgtcggg tcgagggcat ccgcgctcgt cacggccctg
      601 ccggccgcct cgtcgcccca caggcttgga ccggtcgcgg gcgcgatggg aacggccgag
      661 ccgggcagcg catccggatc gtcgtcaggg aacgtcccga acccgcccga tcgaccgtcg
      721 agaggcggcg gcagcggatc gacggcttgc ggcccggggt gaaggtcatc gaacaggacc
      781 ccgaccggac cgaccgagct gcccggctcg accggccaga tcggacttcg ctgcatcccg
      841 gcgaacaccg gatcgagcga cccatcggcc cgctccatcg cccgaccggc gagccgccgg
      901 gcctcgcccg gtcggccaag cgttgcctgc gcctcggccg cgatgacgag tccgaggacg
      961 tcgtccggat gcgccaccag ccactcggtc gccgcctcgc cggcgccgag cacgtcgccg
     1021 gtccgccagc gggcctcggc gaggtcgcgg atggcctcgt cgtcgaggac accgcgtcct
     1081 gccagcgtct cgagctcggt ccgggcgagg ctcagcgaac cgagcagcag gtggacgcga
     1141 gcgaggatca ggtccggatc gcgcggcgcg ccgggacggg ccgatgccga gccaccaccg
     1201 gccgcggtcg acgtcctcga cggcgcgctc atgccggcag cctcagccgt cgcccgaggc
     1261 cgcgcaggtg gcgagccggg gcgaccaccg cgagacgcaa cccctcgtcg cggaacagct
     1321 cgcgggcgag acgctggacc tccgtcgcgt cgaccgccgc gacggcggcg agtgcctcat
     1381 cgagatcgag gacgcgatcg tggagcgctt cctgaccgcc gatccacgaa gcgaggtggc
     1441 gcgtctcgtc cattcgcagc tccaggccgc ccgacaggta ggccttggtc ttggccagct
     1501 cgtcggccgg gacgggctcg tcgcggaggc gggcgagctc agcgaggacc gcgtcgaggg
     1561 cggccggcag gcgctccggg tcgaccccgg cggacacctc gagcgagccc gcatcggcgt
     1621 agtcgaccag ccctgacgag acgtcgtagg ccaggccctg ggcctcgcgg accgagagga
     1681 agagccggct cgacattccg tctccgagca cgccgttgag gaccgccagg atccaggcgt
     1741 cggggtggtc gcggcggagg gccggtacgc cgacgatcag ctgggcctga gtcgtatcgc
     1801 gtcgcgcccg caggagcgcc cgctcgccgg ccggcagctg cggtgcggcg gcatagccgg
     1861 gaatcgcgcc gttgcctgtc ccgaaggccg cggccgccag gtcgacggcc tcgcgatggt
     1921 cgacgtcgcc ggccacggca atcaccgtgt tggccggccg atacatcgtc cgccagaagc
     1981 tccggatcgt gtccgacggg agggcccgga tcccgtcctc gtcgccgcag atctcgcggc
     2041 cgagcgagcc cgacccgaac atcgcctgct ggaagagcgt ctgggcatac tcggccggat
     2101 cgtcgagata ggagcggatc tcctcgacga tcacggtccg ttcgccgtcg atctccgctt
     2161 cgtcgagctt cggccggacg atcagctcac cgaggacgtc cattgcccgg gtcgcctcgc
     2221 ggcgcgggac gcgaacccag tacacggtgg attcccggtc ggtcgcggca ttgaacgacc
     2281 cgccgacgcc ctcgatcgcc tcggagatcg cccgggtcgt ggggaaccgc tcggtcccct
     2341 tgaaggtgat gtgctccatg aaatgggcga ccccggcctc ctcgggcgtc tcgaggcgcg
     2401 agccggcgag gacgtaggcc gcgatggaga ccgaacgcgc gcccggcaac ctggcgctga
     2461 tcacgcgcgg cccaccggcc agcgcggtac gttcgaacat cggggccgat ggtacaggaa
     2521 gggcccgtca cggccgggcg agcccggtca agggaatcgg cgtgccgcgt cagcctcggc
     2581 gacggatccg tcgggaccgc gttgcgtgga gcgcacatgt cgcgtcgcca gacgacggcg
     2641 agtctggaaa tcggtctgag ttgtgcgtgt cgtgcaacga cacgccgcca cgcgagctgg
     2701 atgatctgag ctcgtgtcag atcgttgcgc gatacgcaag tcctggactg ctcgacgggc
     2761 atcgacgctc gacatgtgcg tgggacgcaa cgaggcctcg acggattgcg gcgcgtgcaa
     2821 cgttcggccc gtcgcagggt ccggaccggc cggaccggtc gacgaccagg ggttccccga
     2881 ctctcgcccc cggccggtcg ccgcgcccgt gtcatccggc acggagggcg ggcccattcg
     2941 gaccttcccg gaggtcatcg gcagactatc cttcgtgtca gttcgaccgg gagaggatcc
     3001 gcgggccatc gcccgggcac gaggccggac ggcccgtgcg ggtcgggacg tgtcgcgcta
     3061 tgcgcggcgt gaggaggagc attcatggca agcacacggc tccggctggt gagcctgctc
     3121 gcggcgacgg ccatcgtcgt cggcgcgtgc agctcgacgc cgaaggcgag cggtccggcc
     3181 gcgagtggcg gcgtcgccaa cgactgcgca gccggctccg ccaagggtgg cggaaccggg
     3241 acgctgcagg tccccgaggt cgtcagcggg aagttcaacg tcgcgatggt cctgatcgga
     3301 ccgcacgacg acggcggctg gagccaggcc cactacgagg gcctccagta cctctgccag
     3361 cacctggccg acagccacgt cgcctacatc gaactcgtcc ccgaggggac cgactcggag
     3421 caggtcttcc gaagcctcgc gcgaaagggc ttcaacttca tcatcgggac gtccttcggc
     3481 tacatggacc cgatggagac cgtcgccaag gacttcccgg acgcgacgtt cctgcacctg
     3541 accggctaca agtcgaacgg caagaacttc ggcaacttct tcggcgccgt cgaggacttc
     3601 aagtacctcg ccggcatgct cgcgggctcg cgggcgcaca aggacggcat cccgaagatc
     3661 ggctatatgg cgacattccc gatcccggag gagatccggc tcggcaacgc gatcgcccgc
     3721 ggcgtcgcgg tgacctgccc cgaatgcaag atcgacgtcc gcttcatcaa cacctggcac
     3781 gatccccaga aggaacgtga cggggcggct tcgctgttcg acgccggggc cgatgtcgtc
     3841 ttcaccggtg cggatacccc ggccgtggcc gacgtggccg cggagaaggg caagtgggcc
     3901 gtgacgtacg accatccctc ctcctgcaac aaggacagct gcctgacggc gccgtactgg
     3961 atctggggtc ccgagtacac ccggatcggc cagcaggtga aggacaagaa atacacggcc
     4021 ggctacgagt acttcgacgc gaattccaag gccatgggcc tgttcgggtt catggacggc
     4081 cagaccccgc agaagggggt cgccgacctg ccgccggccg acgtgaccgc ggtcaaggac
     4141 ctgctggcca agatgctcgc cgggacgtcg gaccggttcg aggtcttcgc cggaccgctc
     4201 aaggacaaca ccggcaaggt gctcctcaag gccggtgaga agctcaagca gtccgacctc
     4261 gaccagttcc cgcccggcgc tccgggctcg gagtgcaccg tctgcatgta ctggtggaac
     4321 gagaacatca ccgttcccct ccccaagctg agctgacgat ggcgttcgag gcccgcgggc
     4381 gactgcccgc gggcctcgct gcatcccgga cggctgatcg atgaccgaag aacccgccgc
     4441 gcggccgctc gcggtcgagg cgcgcgggat cgtcaagacc ttcccgggcg tccgcgccaa
     4501 cgaccaggtc gacttcgacc tgcgtcgggg cgaggtccat gccctgctcg gcgagaacgg
     4561 cgccggcaag agcaccctga tgaacgtcct ggccgggctg taccgccagg atgccggcga
     4621 gatcctcgtg gacggcgcgc cggtcgcctt cggctcgccg cgcgacgcga tcgcggccgg
     4681 gatcgggatg gtccaccagc acttcacgct cgtcccgtcg cagacggtga ccgaaaacgt
     4741 cctgctcggt ctcgatcgtc cgcggttctt cctccgcgag cgccagttcg aaggcgaggt
     4801 cgcagcgctc gccacccggt tcgggctgca tgtcgacccg cgggccaaga tctggcagct
     4861 gtcggtcggc gagcagcagc gggtcgagat cctcaagctc ctctaccgcg gcgcccggat
     4921 cctgatcatg gacgaaccga ccgcggtcct cgctccccag gaggcggatg agctcttcgt
     4981 gaccctccgc tcgatgacgg ccgaaggccg atccgtcgtc ttcatcagcc acaagctcgg
     5041 cgaggtcctc gcgatcgccg accgggtcac ggtcatgcgc cgcgggcggg tgacggcggc
     5101 cgggctgccg acggccggag cgacgaaggc cgaccttgcc cggttgatgg tcggccgcag
     5161 cgtgatcgat gcgcccgatc ggacgccgtt cgcccccggc gcggtggtcc tcgccgtcca
     5221 cggcgtgtcc gcgctcaacg accgcggcct gccggcgatg cgggacgtct ccctcgacgt
     5281 ccgttccggc gagatcgtcg ggatcgccgc ggtcgccggc aacggccaga gcgagctcgc
     5341 cgaggtcgtc accggcctgc ggccctgcca gggcgagatc ctcgtcgacg gcgattcggt
     5401 cgcgaaccgg ccggcgtcgg tcgtcctgcg ccacggcgtc gcccacgttc ccgaggaccg
     5461 gacgaccgtc ggcaccgccc cgaacctgtc gctgaccgac aacctgatca tgaagcgtta
     5521 ccgcgacgcc ccggtcgccc acggcctcct tgtcgacgag gcggcggcgc gagcgttcgc
     5581 cggcggactg cgcgacacgt accggatcgc gtccccgaac gtcgacacgc cggcccggat
     5641 gctgtccggc ggaaacctgc agcgggcgat cctggctcgg gaaatcgaga cgaacccggg
     5701 gctcctcatc gcggtccagc cgacgcgcgg gctcgatgtc ggcgcgatcg aatcggttca
     5761 ccgccttctc ctcgacctgc gggcgggcgg cggcgccatc ctgctgatct ccgaggagct
     5821 cgacgagatc ctcgccctcg ccgaccgggt cgacgtcatg tacgaaggcc gggtcgtcgg
     5881 ctcgatgccg gtcgaggagg ccgagatcgg caggatcggg ctgctgatga ccggcggcag
     5941 cgaggcggac cgcgacgcga caccggtcgg cgccgcgccc ggcgtggctc ggtgaggatc
     6001 cacctcgagc cgcggaccac gacgccgcgc tggttgtcgc cgctcgcgac gctcggcgcc
     6061 gtcgccgtcg cgctcgtgat ctccggcgcc gtcatcggcc tcgtcggcgg cgacccggtc
     6121 cgctcctacc tccacatcgt cgaggcgtcg ttcgggagca tcggcgtcct gtccgacacg
     6181 ctcgtcaagg cgacgccatt gatcctgacc gggctcgcct gcgcgctggc gttccggatg
     6241 cgcctctgga acatcggggc ggagggccaa ttcctcctcg gcgcgtgggg cgccagcgcc
     6301 gtggtcctct tcccgctcgt cccgagcggt acgcccgcga tcatcctgat ccccctgatg
     6361 atgctggcgg ggatgctcgc cggcggtctg tggggcctga tcccgggcgc ccttcgagcc
     6421 ctgctcggcg tcaacgagat catcgtgacc ctgatgttca actacgtcgc cctcttctgg
     6481 gtccagttct gggtcttcgg accctggagc gaagggggct tccagcagac acacccgttt
     6541 ccacctcagg cgtggctgcc ccgcctgacc gacttctcgt ccgtcgtccc gggcttcgcc
     6601 gggctgaccg tccacgccgg gctcctcttc gggctcgtgg cggccgggct gctctggctc
     6661 ttcctcgaac gatcccggtt cggctacgag atccggctca tcggcgacag cccgcgggcc
     6721 gcggagtacg cgggaatcaa catcaagcgg cgtctgatca tcgtgttcgc gatctcgggc
     6781 gcgctcgccg ggctggccgg gatgtccgag gtcaacggtg ccgtccatcg cctccaggac
     6841 cacatctcgc ccggctacgg gttcacggcg gtgatcgtcg cctacctcgc caagttccgg
     6901 ccgggccggg tgatcgtggc ggcgatcctg ttcggggcgc tgatcctggc cggtcgcgag
     6961 atccagccgt ccggcgtccc ggtgatgatc caggggatca tcctgttcac cctgatcgtc
     7021 ggcgacgtcc tcgtccgcta ccggatccgg atcacgcgcg gcggcccggc accggcgggt
     7081 ccgctcacgc ccgcaacggg gtcgtcgatc gggcccgccc cgcgcccgga ggcacgcggc
     7141 tgatggacct cgggctgatc gccgcggccg gggtcgcgac gggaaccgtg ctgttgttcg
     7201 ccgcgatcgg cgagatcttc gccgaacggg ccggcgtgat gaacctcggc gtcgaaggca
     7261 tgatgctgat gggcgccgtg gccggcttct cggtgaccgg cgcaacgggc aatccctgga
     7321 tcggcgtgat ggcggccatg ctggtcggcg ggttgttcgg gctgctccac gggatcgtga
     7381 cgatccacct ccgggccgac caggtcgtgt ccggcctcgc cctgaccttc ctcgggacgg
     7441 gcctcgcccg cgtcctcggc gagggactca cgaacacgaa cgtcgagcgc ctcccggcgt
     7501 tcacgattcc ggtcctcgcc aacctcccga tcgtcgggcc gatcgcgttc cagaaccaga
     7561 gcattctcgt ctacgtcggc ttcctgctcg tgccgctgac gtggttctgg atcgcgcgga
     7621 cgcggccggg cctccacctg cgggccgtcg gcgaatcgcc cgcggcagcc gacgtccagg
     7681 ggatcggcgt gtaccgcctc cgcctcgtct acgtcgtgtt cggcgggatc ctcgccgggc
     7741 tggccggggc gacgatcacg ctggcggtcg cgcccggctg gttcggcgac cagaccgtca
     7801 acagcaaagg ctggatcgcc atcgggctcg tgatcttcgc ccgctggagc ccgatctggg
     7861 cggccttcgg cgcctacctg ttcgcggcga tctcgccgtt catcctcgcc ctccaggggg
     7921 tcccgacgat tctcggggtc gagaacccgt tcctgcccgg acgctcggcg acgttcttcc
     7981 tcgagatgct gccgtacctg ttcgtggtcg gcgtcgtcat cctcggctcg cgctcagccg
     8041 cccggcggcg gatcggcgcg ccggctgcgc tggggctgcc gtatgtccgg ggcgagcgcg
     8101 gtctctgagt cgccggccta ggcctcgatg tcgggctcgg tcgtgcggcg agccggctcc
     8161 gggtcgtcca tgaccggatc gccgaccggg tcatcgtcgc cgagaccggt cagctccatc
     8221 tggacgaacc cgctcgtgtc gaaggtcagc tggagggccg
//
LOCUS       DATAIW010000503         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_235085, whole genome shotgun sequence.
ACCESSION   DATAIW010000503 DATAIW010000000
VERSION     DATAIW010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235085"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VHM48_14875"
     CDS             <1..1160
                     /locus_tag="VHM48_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2756751.1"
                     /translation="LGGDANRRASTPGGPATPPVRQESSPPPTRPVPTRTKSRRTTTD
                     PVDVEPSDEELAELIASVTPSAHRDSVATPNRLATRGRAQVASDALLTGARRSTLRNR
                     LTLVAGGIAIVAIVAFAGYRFGGGGLAASLPAASPAASGSIDLAAVSAYMQRIATNSK
                     DTEALQGLADLYYGAADYASATDFLNRILADDPQNIRALLGLGAAAFNDGDSTAAETA
                     WKKVVAIDPKSVEAHYDLGFLYLNLNDLVSVKREWELVVQLDPGSDVAKNVQDHLDAI
                     AAQASPSAGSSSTPAASPAASPVPSGSARRLRSVSRRDPVGSASGATWARPRAAGRVA
                     VPTLSRWALGVTEAMSSASSSSDGSTSSGVELVRRPLVRAGTGRVGGGEDS"
     gene            complement(1734..>2588)
                     /locus_tag="VHM48_14880"
     CDS             complement(1734..>2588)
                     /locus_tag="VHM48_14880"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX2756752.1"
                     /translation="TSCHGDPSTVNQTGSGTPLTPRADAREASHGSPNRGLLTAPYRD
                     RDLEPDGEPYAAANFALCYLCHAERPFADPNVDPSAPDTAFPLHGLHLTSIGGQSGAS
                     LSIDTPGAGQGIAICSECHFRSHSTALAYRPGDLTPTARGNGAEGLVNFAPAVEAVGI
                     IDPTWTRPDAEGQGSCTLTCHGFTHDPASQSYAVAPATGFTASPTSGPAGTDGLTVRF
                     TDATRYADDATATWSWDFGDGTTSTERSPTHVYLAAGSRTVRLTVQRTSDGLATTMSR
                     ASYIVVAP"
BASE COUNT          338 a          916 c          958 g          376 t
ORIGIN      
        1 ccctcggcgg tgacgcgaac cggcgggctt ccactcctgg cggaccggcg acacctccgg
       61 tccgtcagga gtcttctccg cctccgacgc ggcccgtccc gacgcggacc aagagccgcc
      121 gcaccacgac cgatccggtc gatgtcgagc cgtccgacga ggagctcgcc gagctgatcg
      181 catcggtgac gccgagcgcc catcgcgaca gcgtcgcaac gccgaaccgc ctcgcaaccc
      241 ggggacgcgc ccaggtcgcg tccgacgcgc tcctgaccgg agcccggcgc tcgacgctcc
      301 gcaaccggct gacgctcgtc gccggcggca tcgcgatcgt cgcgatcgtc gccttcgccg
      361 gctaccgctt cggcggcggc gggctcgcgg cgtcgctgcc ggcagccagc ccggccgcct
      421 cggggtcgat cgacctcgcc gcggtcagcg cctacatgca gcggatcgcc accaattcga
      481 aggacaccga ggccctccag gggctcgccg acctctacta cggcgcggcc gactacgcca
      541 gcgccacgga cttcctcaac cggatcctgg ccgatgaccc gcagaacatc cgggccctcc
      601 tcggactcgg cgcggcggcc ttcaacgacg gcgactcgac cgctgccgag acggcctgga
      661 agaaggtcgt cgccatcgac ccgaagtccg tcgaggcgca ctacgacctc gggttcctgt
      721 acctcaacct caacgatctc gtcagcgtga agcgtgagtg ggagctcgtc gtccagctcg
      781 atccgggttc cgacgtcgcg aagaacgttc aggaccatct cgacgccatc gccgcccagg
      841 cctcgccgag cgcaggctcg agcagcacac ccgccgcctc ccccgccgct tcgccggtcc
      901 cgagcgggag cgccaggcgg ttgcggagcg tcagccgtcg ggatccggtc gggagtgcgt
      961 cgggcgcgac ctgggcgcgt ccccgggccg ccgggcgggt cgccgttccg acgctgtccc
     1021 ggtgggcgct cggcgtcacc gaggcgatga gctcggcgag ctcctcgtcg gacggctcga
     1081 catcgagcgg agtggagttg gtgcggcggc ccttggtccg cgccgggacg ggccgcgtcg
     1141 gaggcggaga agactcctga cggaccggag gtgtcgccgg tccgccagga gtggaagccc
     1201 gccggttcgc gtcaccgccg agggcgacgg tccgcgcgag tgcggcaggg tcggtgagca
     1261 gcgcgtaggc ctcgtctgcg gaggaggcct ggcgccgggc ccacgcgccg agttggcgcg
     1321 gagcggaagc gaggaactgc tcgacggact cgtgtgccgc cgcgatgtcc tcggaggtgg
     1381 ttgcatcgtc gagcccgaga cgggccagga ggtccgtccg gacgtcatcg agcgttgcgg
     1441 agcggccggc cgatgcggtc atcgccgcat ctccgtcgcc ggcaccactc ggttgtgccg
     1501 ttgctgccga ttcatcggga ctgccctcgt ttcgatcgtg ccgcgtgaca cctgcggctt
     1561 gacacctgcc acgattgtcc ggagcgaggg ccgcggatcc catccgcctg cgggcacatt
     1621 tcgggtatgc ggggcacgcc cgctcggtac ggcctgagca cgggcctatg ccgttcgata
     1681 cagatggcgg cctggtcccg atcgtcagag agagcctggc gcgggggcga cggtcagggc
     1741 gcgaccacga tgtagctcgc ccgggacatc gtggtggcga ggccgtcgct cgtccgctgg
     1801 accgtgagcc ggaccgtcct cgagcccgcc gcgaggtaga catgggtcgg gctgcgttcg
     1861 gtcgacgtcg ttccgtcccc gaagtcccag ctccacgtcg ccgtcgcgtc gtcggcgtag
     1921 cgggtggcgt cggtgaaccg cacggtcagg ccgtcggtcc ccgcgggacc gctcgtcggg
     1981 ctcgccgtga acccggttgc cggggcgacc gcgtacgact ggctcgccgg gtcatgggtg
     2041 aagccgtgac aggtcagcgt gcaggagccc tgtccctcgg cgtctggccg ggtccacgtc
     2101 gggtcgatga tgccgacggc ctcgacagcc ggggcgaagt tcacgagacc ctcggcgccg
     2161 ttgccgcgcg cggtcggggt caggtcgccg ggccggtagg cgagcgcggt ggaatggctc
     2221 cggaaatggc actccgagca gatcgcgatg ccctggccgg cgcccggcgt gtcgatcgag
     2281 aggctcgccc cgctctgccc gccgatgctg gtcaggtgga ggccgtggag cgggaacgcg
     2341 gtgtcgggcg cgctcgggtc gacgttcggg tcggcgaacg gccgctccgc gtgacagagg
     2401 tagcacaggg cgaagttggc ggccgcgtat ggctcgccat ccggctccag gtcgcggtca
     2461 cggtacgggg cggtcagcag gccgcggttc ggcgagccat gactcgcctc gcgggcgtcg
     2521 gctcggggcg tcagtggcgt gccggagccg gtctggttca cggtcgacgg atcgccgtgg
     2581 cagctcgt
//
LOCUS       DATAIW010000504         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_205216, whole genome shotgun sequence.
ACCESSION   DATAIW010000504 DATAIW010000000
VERSION     DATAIW010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205216"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VHM48_14885"
     CDS             complement(<1..726)
                     /locus_tag="VHM48_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2756753.1"
                     /translation="MTDEPTTSDSHLAPADTAPPGPHRLTLMTVHAHPDDETISAGGA
                     MAKAIAAGHRVVLVTGTRGEQGEIVVPAMDTPDNHRRLGELRAAELEAAMGELGVTEW
                     ENLGYRDSDMMGRDGNHDPRTFWQADLDEAAGRLTWLVRRYQPDVVTTYNSFGGYGHP
                     DHIRVHDVSIRAFARAGDPAWYPKHLENGLAPWAPSKLYETAIPASVREAIREAMKEA
                     GKPSFWDPPEDATPEQIAEHEKFL"
     gene            complement(773..1210)
                     /locus_tag="VHM48_14890"
     CDS             complement(773..1210)
                     /locus_tag="VHM48_14890"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2756754.1"
                     /translation="MTPRRLFFRIGWAVHRAMWRLSGHRIGTSAAGPDGVGTLFLLST
                     GRRSGAIRRNGLYYVEDGASFAVVASNAGDDADPQWWRNLQATPAATIEVGATALAVR
                     AREATDAERDRIWPRFVARYPVFADYRSRTTRAIPVVILEPRR"
     gene            1255..>1908
                     /locus_tag="VHM48_14895"
     CDS             1255..>1908
                     /locus_tag="VHM48_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2756755.1"
                     /translation="MATPDAPVSGPLARIRVIDCSTVLAGPYATMLLADLGADVIKVE
                     PPDGDATRGWGPPWVGSVEDGTRTAAYALAVNRNKRSIRVDLRQPDGAAVLRRLLTDA
                     DVLVENIRPGGLDRLGFDDGTLAALNPALVHLSITGYGPGAPDPARPGYDFVIQAESG
                     LMSITGAPDVDGGAPTKVGVAISDVVTGLNGAVAILAALLARGGADVRGQRIDLSLLG
                     "
BASE COUNT          248 a          699 c          674 g          287 t
ORIGIN      
        1 gaggaacttc tcgtgctcgg cgatctgctc cggcgtcgcg tcctccggcg ggtcccagaa
       61 gctcggcttg ccggcttcct tcatcgcctc gcgtatcgcc tcgcggaccg acgccgggat
      121 cgccgtctcg tagagcttcg aaggcgccca cggggcgagc ccgttctcga ggtgcttcgg
      181 ataccaggcc gggtcgccgg cccgggcgaa cgcccggatc gacacgtcgt ggacccggat
      241 gtggtcggga tggccgtatc cgccgaagct gttgtaggtc gtgacgacgt cgggctggta
      301 gcgccggacc agccaggtca ggcggccggc ggcctcgtcg aggtcggcct gccagaacgt
      361 ccgcggatcg tggttcccgt cgcggcccat catgtccgag tcgcgatagc cgaggttctc
      421 ccactcggtc acgccgagct cgcccatcgc cgcctcgagc tccgccgcgc gcagctcgcc
      481 gagccggcga tggttgtccg gcgtgtccat cgccgggacg acgatctcgc cctgctcgcc
      541 gcgggtgccg gtcacgagga cgacccgatg cccggcggcg atcgccttgg ccatcgcgcc
      601 ccctgcgctg atcgtctcgt catcaggatg ggcgtggacc gtcatcaggg tcagccgatg
      661 tggccccggg ggcgccgtgt cggcgggggc gaggtggctg tcggaagtcg tgggttcgtc
      721 ggtcattgcc gcatcatcgc attgacctcc gccgggcgcg acgcggcgcc cgtcagcggc
      781 gcggctcgag gatgacgacc gggatcgcac gggtcgtccg gctccgatag tcggcgaaca
      841 ctgggtacct ggcgacgaac cgcggccaga tccggtcacg ttccgcgtcc gtcgcctcgc
      901 gggcccgaac ggcgagggcc gtcgcgccga cctcgatcgt cgcggccggc gtcgcctgga
      961 ggttgcgcca ccactgcgga tccgcgtcgt cgcccgcgtt ggacgcgacg accgcgaacg
     1021 atgcgccatc ctctacgtag tacaggccat tccgccggat cgcgccggag cgccgtccgg
     1081 tcgagagcag gaacagggtc ccgacaccgt cgggtccggc cgccgaggtg ccgatccggt
     1141 gacccgacag gcgccacatg gcgcgatgga cggcccagcc gatccggaag aagaggcgcc
     1201 gcggtgtcac caacccatga tcgtcgatcg ggccgcgtcc taccatcgac ggcgatggca
     1261 actcccgacg ctccagtttc cggcccactt gcccgcatcc gggtgatcga ctgctcgacg
     1321 gtcctcgccg ggccgtacgc gacgatgctc ctggcagacc tcggagcgga cgtgatcaag
     1381 gtcgagccgc ccgacggcga cgcgacgcgc ggttgggggc cgccgtgggt cgggtcggtc
     1441 gaggacggga cgcggaccgc ggcctatgcg ctggcggtga accgcaacaa gcgctcgatc
     1501 cgggtcgacc ttcggcaacc ggatggggcg gccgtcctgc gccgcctcct caccgacgcc
     1561 gacgtcctcg tcgagaacat ccggccgggc ggcctcgacc gcctcgggtt cgacgacggc
     1621 accctcgccg ccctgaaccc ggcgctcgtc catctctcga tcacgggtta cggcccgggc
     1681 gcccccgatc cggcccgccc cggctacgac ttcgtcatcc aggccgagtc gggcctgatg
     1741 tcgatcaccg gcgcgcccga tgtcgacggt ggcgcgccga cgaaagtcgg ggtcgcgatc
     1801 agcgacgtgg tcaccgggct caacggggcc gtcgccatcc tcgctgccct gctggcccgc
     1861 ggcggcgcgg acgtgcgcgg ccaacggatc gacctgtccc tcctcggc
//
LOCUS       DATAIW010000505         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_205420, whole genome shotgun sequence.
ACCESSION   DATAIW010000505 DATAIW010000000
VERSION     DATAIW010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205420"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHM48_14900"
     CDS             <1..349
                     /locus_tag="VHM48_14900"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX2756756.1"
                     /translation="RMKKWPVLVWVDVVLHKIFPIIVVLDWILDPPRRRLVNRDVVNL
                     LVYPLIWVVLTMIRGALDSWYPYPFLDPAKGGYGQVVITIVAVLVGFIAIALAIMALG
                     NWRRDASPERLPA"
     gene            complement(351..2039)
                     /locus_tag="VHM48_14905"
     CDS             complement(351..2039)
                     /locus_tag="VHM48_14905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2756757.1"
                     /translation="MDLHFLGGATTVTGSQYLLETGRARVLIDCGMFQGSPNEAMRNR
                     VPLAYDPAILDVMLLTHAHLDHCGLIPHVVNEGYRGRIIATKGTCELAELVLLDSGKL
                     QQEFAKREARWETKHPDQVAADDRKEEDLYQAAVDLAAGRIAPPPVRENVDDAPPPLT
                     PHATSESAILATAGVSGGVPGATGSRPADPEAALRAQPAEMDIDLDEPLYTADDAQAA
                     VEHFDAIGYDEEREVAPGVHATFLDAGHILGSAIIRLRIEAGDPANGGPERETTIVFS
                     GDLGRPNTPIIRDPTVLTSADYVLCESTYGGREHEPEDEAIRILAETVNLVSEAGGVL
                     LVPSFAIGRTQEIVYALDRLLEDGKIPQLPLYLDSPMASKASDIYRRHADYFDEDARR
                     LLQSGETPLDYPDQTETRDVHDSEKIAQAKRPYMIVASNGMLTGGRVLGHLRNLIGDP
                     AATLLFVGYQGEGTLGAHLQAGVKTVKLDGQPREVRCRIRSISGFSAHADEPELLDWL
                     RHFADGKTAGGAGFPKTIFLVHGDPEAQIAIEPKVRALGFTTKIPHWRETVTLS"
     gene            complement(2141..2362)
                     /locus_tag="VHM48_14910"
     CDS             complement(2141..2362)
                     /locus_tag="VHM48_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756758.1"
                     /translation="MRLRIDWSERAAARMDFASSSAAPASWTYTGPDVPQPGGENARM
                     NLWLFRGQPPSDGQGIEIVVTDFTFTPLS"
     gene            complement(2423..>2903)
                     /locus_tag="VHM48_14915"
     CDS             complement(2423..>2903)
                     /locus_tag="VHM48_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2756759.1"
                     /translation="IVIGGAAGALPPVIGWAAVTGNVGVPALVLFALVFYWTPPHFWA
                     LSLRIRKDYAAAGVPMLPVVRGIPETTRQIGLYTILMVAISLVLFAVARMGPIYLVAA
                     VVLGALFLRQAWMLWRRGDSEEESTAGAIRLYKYSISYLTLLFAAIALDTLVLAAVG"
BASE COUNT          422 a          961 c         1064 g          456 t
ORIGIN      
        1 gcgcatgaag aaatggcccg tgctcgtctg ggtcgatgtc gtcctccaca agatcttccc
       61 gatcatcgtc gtcctcgact ggatcctcga tccgccgcga aggcgcctcg tcaaccgcga
      121 tgtcgtgaac ctgctggtct atcccctcat ctgggtcgtc ctcacgatga tccggggcgc
      181 gctggacagc tggtatccgt acccgttcct cgatccggcc aagggtggct acggccaggt
      241 cgtgatcacg atcgtggcgg tcctggtcgg gttcatcgcg atcgcgctcg cgatcatggc
      301 cctcggcaac tggcgccggg acgcgagccc ggagcggctc cccgcctgac tcagctcagc
      361 gtcaccgtct cccgccagtg cggaatcttc gtcgtgaagc cgagcgcccg gaccttcggc
      421 tcgatcgcga tctgggcctc ggggtcgccg tggacgagga agatcgtctt cgggaaaccg
      481 gccccgccgg cagtcttgcc gtcggcgaaa tggcgcagcc agtcgagcag ctcgggctcg
      541 tcggcgtggg cggaaaatcc gctgatcgat cggatccggc agcggacctc gcgcggctgg
      601 ccgtcgagct tgaccgtctt caccccggcc tggaggtggg cgccgagcgt tccctcgccc
      661 tggtagccga cgaacagcag ggtcgcggct gggtcgccga tcaggttccg gagatggccg
      721 aggacccggc caccggtcag catgccgttg gacgccacga tcatgtacgg ccgcttggcc
      781 tgagcgatct tctccgagtc gtggacgtcg cgtgtctcgg tctggtcggg atagtcgagc
      841 ggcgtctcgc ccgactggag cagccgccgg gcgtcctcgt cgaagtagtc ggcgtgacgg
      901 cggtagatgt ccgacgcctt cgacgccatc ggcgagtcga gatagagcgg cagctgcggg
      961 atcttgccgt cctcgagcag ccgatcgagg gcatagacga tctcctgggt ccgcccgatc
     1021 gcgaacgacg ggacgagaag cacgccgccg gcctctgaca cgaggttgac ggtctccgcg
     1081 aggatccgga tcgcttcgtc ctcgggctcg tgctcgcggc cgccgtaggt cgattcgcag
     1141 aggacgtagt cggccgaggt caggacggtc gggtcgcgga tgatcggcgt gttcggccgg
     1201 ccgaggtcgc cggagaagac gatcgtcgtc tcacgctcgg gaccgccgtt cgcgggatcg
     1261 ccggcctcga tccggagccg gatgatcgcc gaaccgagga tatggccggc gtcgaggaac
     1321 gtcgcgtgga cgccgggggc gacctcgcgc tcctcgtcgt agccgatcgc gtcaaaatgc
     1381 tcgaccgcgg cctgggcgtc gtcggccgtg tagagcggct cgtcgaggtc gatgtccatt
     1441 tccgcgggct gggcgcggag agcggcctcc gggtcggctg gtcggctgcc ggtcgcacct
     1501 gggactcctc cggaaacgcc ggccgtcgcg aggatggccg actcggacgt ggcgtgaggc
     1561 gtcagcggcg gcggcgcgtc gtcgacgttc tcgcggaccg gcggcggcgc gatccggccg
     1621 gccgccaggt cgacggctgc ctggtacagg tcctcctcct tgcggtcgtc cgcggcgacc
     1681 tgatccgggt gtttcgtctc ccagcgtgcc tcgcgcttgg cgaactcctg ctggagcttg
     1741 ccggagtcga gcaggacgag ctcagccagc tcgcaggtcc ccttcgtcgc gatgatgcgg
     1801 ccgcgatagc cctcgttgac gacatgcggg atcaggccgc agtggtcgag gtgggcatgg
     1861 gtcaggagca tgacgtcgag gatcgccggg tcgtaggcga gcgggacgcg gttgcgcatc
     1921 gcctcgttcg ggctgccctg gaacatcccg caatcgatga ggacgcgggc tcggccggtc
     1981 tcgagcaggt actgcgagcc ggtgacggtg gtggcaccgc cgaggaagtg gaggtccatc
     2041 tgcggagtgt ggcaccggcg gccttcgcgc atcgggcgcg tggcggctcg ccggctcgcg
     2101 ccagcgttgc agtccggccg gcgacgtcag gccgggtcga tcacgagagg ggcgtgaacg
     2161 tgaagtcggt caccacgatt tcgatcccct ggccgtccga aggtggctgg ccacggaaca
     2221 gccagaggtt catccgggcg ttctcgccgc ccggctgggg gacgtccggg ccggtgtacg
     2281 tccagctcgc gggcgcggcc gacgagctcg cgaagtccat ccgcgccgcg gcacgctcgc
     2341 tccagtcgat tctgagtcgc accggactcg gtggggcgta ggagcgggcc tccggggcgg
     2401 gcggccgcga gcccgcctga gctcagccga cggccgcgag caccagcgtg tcgagcgcga
     2461 tcgccgcgaa cagcagcgtc aggtaggaga tcgagtactt gtagaggcgg atcgcgcccg
     2521 cggtcgactc ctcctcggag tcgccgcgcc gccagagcat ccacgcctgg cgcaggaaca
     2581 gggccccgag gacgaccgcg gcgacgaggt agatcggccc catccgggcg accgcaaaga
     2641 gcacgagcga gatcgcgacc atgaggatcg tgtacaaccc gatctggcga gtcgtctcgg
     2701 ggatcccgcg cacgaccggc agcatcggca caccggccgc cgcgtagtcc ttgcggatcc
     2761 gcagcgacag cgcccagaag tggggcggcg tccagtagaa gacgagggcg aacaggacga
     2821 gtgccgggac gccgacgttg ccggtgaccg cggcccaccc gatgaccggc ggaagggcgc
     2881 cggccgcccc gccgatcacg atg
//
LOCUS       DATAIW010000506         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_236397, whole genome shotgun sequence.
ACCESSION   DATAIW010000506 DATAIW010000000
VERSION     DATAIW010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236397"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VHM48_14920"
     CDS             complement(<1..256)
                     /locus_tag="VHM48_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2756760.1"
                     /translation="MSDDVDLLITGASLVDGTGAPARSGDLAISGGRIREIRPGDELP
                     TAARTIDAPGKVVAPGFIDLHSHAALMILADPDHGAKARQG"
     gene            493..1140
                     /locus_tag="VHM48_14925"
     CDS             493..1140
                     /locus_tag="VHM48_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2756761.1"
                     /translation="MTTRHGPVARAVERALEDEARVRADLARARRGSGISREIVGKAC
                     GLSRTGIERIESGTRRSTIRELAAFGAVVGLDVRLRAFPAGDPIRDAGQVRLLERLRV
                     RLHPSLRWSTEVPLPMEGDLRAWDAVIRGRAWRLPVEAETVLDDVQATERRLALKQRD
                     GGEDHVILLVADTRRNRRALAAAPGAFADLPMRTREILAALGDGRRPAASGIVIL"
     gene            complement(1142..1606)
                     /locus_tag="VHM48_14930"
     CDS             complement(1142..1606)
                     /locus_tag="VHM48_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2756762.1"
                     /translation="MIDARFGHVNLIARDWRALATFYTDLFGCEPVPPERDYAGPDLE
                     RGTGVPGAALRGIHLRLPGHGPDGPTLEIYQYASMPDGLPAAVDRPGFGHIAFAVPSV
                     ADARAAVLGAGGRAIGEVVTLRTADGRFVTWTYVTDPEGNIVELQSWSDTRP"
     gene            complement(1603..>2908)
                     /locus_tag="VHM48_14935"
     CDS             complement(1603..>2908)
                     /locus_tag="VHM48_14935"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEX2756763.1"
                     /translation="VVSPLAGGGTLDLADGLVEVDAAGRIAAVGAVADRPDGAPADVI
                     DLRPWVVLPGMVDLHAHLPQVPNAGLGYALPLLDWLDRLTFPTERSWADPAVAERLAP
                     AIFRAFAAAGTTTVLAYGVVYEAAMEAAFRAAEAHGIRAILGKVMMDRRTYDPTIEPS
                     TILERTLRESGDLARRWHGAADGRLGYAVTPRFAVSCTADLLRESAALAADLGCWWQS
                     HVSEDRAEIAEVGRLFPEARDYVDVYDRAGALGPRSVLAHAIHLSPREEARLAETGTR
                     VAHCPTSNLFIGAGVMPLADRLAAGLSVGLGSDVSGGAELSMFGVMRTGAYAQQALRA
                     TSSAGAATPAPRGPLDWLRMATLDGARALGLDDRIGSIETGKEADLIAVDPGATAPLG
                     PDLDAPGLLDDTALLVGRLIFRAEPSMVRGAWVRGRALEGRS"
BASE COUNT          401 a         1065 c         1071 g          371 t
ORIGIN      
        1 cgccctggcg ggccttcgcc ccgtggtccg ggtcggcgag gatcatcagc gcggcgtggg
       61 agtggaggtc gatgaagccc ggggccacga ccttgccggg ggcgtcgatc gtgcgggcgg
      121 ctgtcgggag ctcatcgccc ggccggatct cgcggatccg gccgccgctg atcgcgaggt
      181 caccgctccg tgccggcgct cccgtcccgt cgacgagcga ggcgcccgtg atcaggagat
      241 cgacgtcatc gctcatcgcc ggtccacctt ccgccggccc gcccggaggg cccggcgctc
      301 gatgcacgcc ggccgggcat tgtgacggtt ggaaggctcg ccgcgggtcg aactgcccgc
      361 gcatcgtgca attcgacggc cgatcctcgt gaatccggcg tgccgggtcg ccgcgcattc
      421 tggcgggaca tcacaacgcc caaagagcgt gcagtcaatc gccagtgaag ggccgcggcg
      481 caccatcgca tgatgacgac ccgacatgga cccgtggcgc gagccgtcga gcgcgccctc
      541 gaggatgagg ctcgtgtccg ggccgatctg gctcgggcac ggcgtgggtc cgggatcagc
      601 cgggagatcg tcggcaaggc ctgcgggctt tcgaggaccg ggatcgagcg gatcgaatcc
      661 gggactcgac gatccacgat ccgcgagctc gcggccttcg gggcagtcgt ggggctggac
      721 gtgagactgc gggcatttcc cgccggcgac ccgattcggg acgcgggcca ggtccggctg
      781 ctggagcgac ttcgggtccg tctccacccg agcctgcgct ggtcgaccga ggtgccgctc
      841 ccgatggagg gcgacctccg cgcctgggat gcggtgatcc gcggccgggc ctggcgactc
      901 ccggtcgagg cggagaccgt cctcgacgac gtccaggcca ccgagcgccg cctcgccctg
      961 aagcaacgcg acggcggcga ggatcacgtg atcctcctcg tcgcggacac gaggcgaaac
     1021 cgtcgggcgc tcgcggcggc gcccggagcg ttcgcggatc tgccgatgcg gacccgcgag
     1081 atcctcgccg cgctcggtga cggccggcga ccggccgcca gcgggatcgt gatcctctga
     1141 atcacggtcg cgtatccgac caggactgca gctcgacgat gttgccctcc gggtcggtca
     1201 cgtaggtcca ggtcacgaac cggccgtcgg ccgtccggag cgtgacgacc tcgccgatgg
     1261 cgcgcccgcc cgcgccgagg acggcggctc gggcgtccgc caccgatggg acggcgaacg
     1321 cgatgtgccc gaagcccggg cggtcgaccg ccgccggaag gccgtcgggc atcgacgcgt
     1381 actggtagat ctcgagggtc ggcccgtcgg ggccgtggcc cggcagccgg aggtggatgc
     1441 cgcgcagggc cgcgccgggc acgccggtcc cgcgctcgag gtcgggcccg gcgtagtccc
     1501 gttccggcgg cacgggctcg cagccgaaca ggtccgtgta gaacgttgcc aacgcccgcc
     1561 agtcgcgggc gatgaggttg acgtgaccga accgggcgtc gatcacgatc gtccctccag
     1621 ggcgcgcccg cggacccatg cgccgcggac catcgacggt tcggcccgga agatcaaccg
     1681 gccgaccagc agcgcggtgt cgtcgagcag gcccggggca tccaggtccg ggccgagcgg
     1741 cgccgtcgcc cctgggtcga ccgcgatcag gtcggcctct ttgccggtct cgatcgagcc
     1801 gatccggtcg tcgagcccga gcgcccgggc gccgtccagg gtcgccattc gcagccagtc
     1861 gagcggcccg cggggtgccg gcgtcgcggc gccggccgac gacgttgccc gcagcgcctg
     1921 ctgcgcgtac gccccggtcc gcatgacccc gaacatcgag agctccgcgc cgcccgacac
     1981 gtccgagccg agcccgaccg acaatcccgc tgccagccga tccgccaacg gcatcacgcc
     2041 cgcaccgatg aagaggttcg aggtcgggca atgggcgacc cgcgtcccgg tctcggccag
     2101 acgcgcctcc tctcgcggcg acaggtggat cgcgtgggcg aggaccgatc gcggaccgag
     2161 cgcacccgcc cggtcgtaga cgtcgacgta gtcgcgggcc tccgggaaca ggcgaccgac
     2221 ctccgcgatc tcggcacggt cctccgagac atggctctgc caccagcagc cgaggtcggc
     2281 ggcgagggcg gccgactcgc gcagcaggtc cgcggtacac gaaacggcga atcgcggggt
     2341 caccgcatag ccgagccggc cgtcggccgc gccatgccag cgtcgcgcca ggtcgccgct
     2401 ttcgcgcagc gtccgctcga ggatcgtcga cggctcgatc gtcgggtcgt aggtccgccg
     2461 gtccatcatc accttgccga ggatcgcccg gatcccgtgc gcctccgcgg ccctgaacgc
     2521 cgcctccatc gccgcctcgt agacgacgcc gtaggcgagg accgtcgtcg tcccggccgc
     2581 agcgaacgcc cgaaagatcg cgggtgccag ccgctcggcc acggccgggt cggcccacga
     2641 gcgctcggtc gggaacgtca ggcggtcgag ccagtcgagg agcggcaggg catacccgag
     2701 cccggcgttc gggacctgcg ggagatgggc gtggaggtcg accatccccg gcaggacgac
     2761 ccatggccgg aggtcgatga cgtccgccgg cgccccatcc ggccgatctg cgacggcgcc
     2821 gacggccgcg atccgcccgg ccgcgtcgac ctcgacgagg ccgtcggcca ggtcgagggt
     2881 gccgccgcct gccagcggcg agacgact
//
LOCUS       DATAIW010000507         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_206541, whole genome shotgun sequence.
ACCESSION   DATAIW010000507 DATAIW010000000
VERSION     DATAIW010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206541"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VHM48_14940"
     CDS             complement(<1..294)
                     /locus_tag="VHM48_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX2756764.1"
                     /translation="MPNRLAGETSPYLLQHAHNPVDWYPWGPEALARARELDRPIFLS
                     IGYAACHWCHVMERESFEDAATAADLNRDFVAIKVDREERPDLDQVYMGAVQAM"
     gene            complement(321..857)
                     /locus_tag="VHM48_14945"
     CDS             complement(321..857)
                     /locus_tag="VHM48_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2756765.1"
                     /translation="MSPAIVLDASVALTFVLRQQGVVDVDARLERWRRSGDRLCVPSS
                     FWLEVSNSLLRRHGFPAGSIIEAIHRLDEFGLETVEVDRAMTLLALDRAERFGLTTYD
                     AAYLAVAEALDATLYTSDRALLSAAGWRGIAVDGAPGRRRSEVKARYGPERPSTWPDY
                     SGASAYLAKLRAEATRAT"
     gene            complement(854..1195)
                     /locus_tag="VHM48_14950"
     CDS             complement(854..1195)
                     /locus_tag="VHM48_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2756766.1"
                     /translation="MRVVTPTDMRKRLGEILDAASAGERILIERDHKPIAWLVSPEDG
                     RRFDEDEQARIERSLAALDRLREFSIEMAAKYPPPDDGLTEAEWLAQERERRADHIEE
                     AVRSIGRPTDE"
     gene            complement(1249..2514)
                     /locus_tag="VHM48_14955"
     CDS             complement(1249..2514)
                     /locus_tag="VHM48_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756767.1"
                     /translation="MDGVATLAFRRSPRTWDALALTVVLLSLGSIAGCTPTGDANSPR
                     PSGTVSRAPARVSEGIASRPPTAGDVSGPPASGSIRIQAIDQPGLFDGPDTIILRDIV
                     TAGAGFTVLGDVGRGITPRIDAWLSPDGQTWERDATVTGLDGVTVNDVEETASGRLVA
                     VGWKQAAALTWSSDDGGRTWQEHVLPGDPHGQAYAATSGSAGTLMLVGGPIGRGPEQA
                     TKLWFSPDGASWSAVAMPDDVFGAIEIRGITATTNGFVAVGGRAPANPIRPGDQTEYR
                     AAAWLSVDGRTWSAATVSDRPMMLGAVAGSEGLIGFGWGEPITRPQTWFSEIGASWIA
                     NPLVSDKSPAVVAHDGLMSWLDSTVIRSGLAKLEWKTSRNGIDWVSLGSVEESRAVGI
                     GGFRAGDPGILQLGGSIDRPAMWLFSIPG"
     gene            2552..3445
                     /locus_tag="VHM48_14960"
     CDS             2552..3445
                     /locus_tag="VHM48_14960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2756768.1"
                     /translation="MRYGLCLPNFTGLASPEAIDAAAAVAERLGWDSVWTTDHVLVDS
                     SEDAADYRTNFDVIETLAWVGARYPTIGLGTSVIVVPQRNAIVLAKELATLDALSGGR
                     VIAGIGVGWSEPEFANLAMADRFAVRGAYLEETIELWRHLWSGSGEPFAGRFHTVSDY
                     RFGPLPAQGVGLPIWIGGRADAALERAGRLADGYHSSASGPESFARRVPVIRAAADAA
                     GRPMPTLSARVRVAFDEPVDSAEPRPYAMRGSAEDVAAEVAKFAALGVEHLAIFFEAD
                     TIEAFLADAERFTAEVAPLGS"
BASE COUNT          538 a         1338 c         1282 g          567 t
ORIGIN      
        1 catcgcctgg acggcaccca tgtagacctg gtcgaggtcg ggccgctcct cgcggtcgac
       61 cttgatcgcg acgaagtccc ggttcaggtc ggcggccgtg gccgcgtcct cgaagctctc
      121 gcgctccatg acgtgacacc agtggcacgc ggcgtagccg atcgacagga agatcggccg
      181 gtcgagctcg cgcgcccggg ccagcgcctc gggcccccac gggtaccagt cgaccgggtt
      241 gtgggcgtgc tggagcaggt acgggctcgt ctcgccggcg agtcggttgg gcattcgcga
      301 atggtaggcg gggcggccgt tcaggtcgcc cgcgtcgcct cggctctcag cttggccagg
      361 taggccgagg cgcccgagta gtccggccac gtcgatggtc gttcgggacc ataccgagcc
      421 ttcacctccg aacgtcgacg gccgggagca ccgtcgaccg caatgccacg ccagcccgcg
      481 gcggagagga gggctcggtc ggacgtgtac agcgtggcat cgagggcttc ggcaacagca
      541 aggtaggccg cgtcgtaggt ggtcaaaccg aatcgctcag ctcgatcgag tgcgagaagc
      601 gtcatggctc gatcgacctc gaccgtttcg agcccgaatt catccagccg atggatcgcc
      661 tcgatgatcg agccggccgg gaacccgtgg cgccgcagga ggctgttcga gacctccagc
      721 cagaacgacg acggaacgca cagccggtcg ccggacctgc gccaccgttc gagccgagcg
      781 tcaacgtcca cgacaccttg ctgtcggagc acgaacgtga gcgcaaccga cgcgtccagg
      841 acgatcgccg gactcattcg tcggtcggtc gcccaatcga cctgacggcc tcttcgatgt
      901 gatcggcgcg acgctcgcgt tcctgggcga gccactcggc ctcggtcaga ccatcgtcag
      961 gaggcgggta tttggcggcc atttcgatgc tgaactcccg gagccggtcg agcgccgcca
     1021 gcgatcgctc gatccgggcc tgctcgtcct cgtcgaagcg ccgcccgtcc tcgggcgaca
     1081 ccagccaggc gatcggcttg tggtcgcgct cgatgaggat ccgctcgccg gccgacgcgg
     1141 cgtcgaggat ctcgccgaga cgcttgcgca tgtcggtcgg cgtcacaact ctcatgggga
     1201 accatcatgg ccagccatca tgaccctgtc aagatcgtcc ttcgagcttc agcccgggat
     1261 cgagaagagc cacatcgcgg gccgatcgat ggacccgccg agctggagga tccccggatc
     1321 tcccgcccgg aagccgccga tcccgaccgc tcgcgactcc tcgacggatc cgagcgagac
     1381 ccagtcgatc ccgttgcggc tcgtcttcca ctcgagcttc gcgaggcccg agcgaatgac
     1441 cgtcgagtcg agccacgaca tcaacccgtc gtgggcgacg acggcgggac tcttgtcgga
     1501 cacgagcgga ttggcgatcc atgacgcgcc gatctcggaa aaccaggtct gcggtcgcgt
     1561 gatcggctcg ccccatccga acccgatcag gccctccgac ccggcgaccg cgccgagcat
     1621 catcggccgg tcactgaccg tcgctgcgga ccacgtacgg ccgtcgaccg acagccaggc
     1681 ggcggcgcgg tactccgtct ggtcaccggg gcggatcggg ttggccggtg cccgcccgcc
     1741 gaccgccacg aatccgttcg tcgtcgcggt gatgccgcgg atctcgatgg ctccgaagac
     1801 atcgtccggc atggcgacgg ccgaccacga ggcgccatcc gggctgaacc agagctttgt
     1861 ggcctgctcg ggtccgcgcc cgatcgggcc cccgaccagc atcagggttc ccgccgagcc
     1921 gcttgtcgcc gcgtaggcct ggccatgggg gtcgccaggc aggacgtgct cctgccaggt
     1981 tcggccgccg tcgtccgacg accatgtcaa cgccgctgcc tgcttccagc cgacggcgac
     2041 gagccgtccg gacgccgtct cctcgacgtc gttcaccgtc acgccgtcca atccggtgac
     2101 cgtcgcatcc cgctcccagg tctggccgtc ggggctcagc cacgcgtcga tgcgaggcgt
     2161 gatccctcgg cccacgtcgc caaggaccgt gaaacctgcg ccggcggtga cgatatcgcg
     2221 gaggatgatc gtgtcgggtc cgtcgaacag gccgggctgg tcgatcgcct ggatcctgat
     2281 cgaaccggac gccggcgggc ccgacacgtc gcccgcggtc ggcgggcggg acgcgatccc
     2341 ttcggagacc cgcgccgggg ctcgggagac cgtgccgctt ggtcgcggcg agttcgcatc
     2401 gccggtcggc gtgcaaccgg cgatcgagcc gagactgagc agaaccacag tgagggcgag
     2461 ggcgtcccag gtccgcgggg agcggcgaaa cgcgagcgtg gccactccat ccatcgcgcc
     2521 gagtatgtca ggcgaccgct aacctgccgt catgcgctac ggactctgcc tccccaactt
     2581 caccgggctc gcctcgcccg aggccatcga cgcggctgcc gcggtcgccg agcgattggg
     2641 ctgggattcg gtctggacga ccgatcacgt cctcgtcgac tcgagcgagg acgccgcgga
     2701 ctaccggacc aacttcgacg tcatcgagac cctcgcctgg gtcggcgccc ggtatccgac
     2761 catcgggctc gggacgtcgg tcatcgtcgt cccgcagcgc aacgcgatcg tcctcgccaa
     2821 ggagctcgcg acgctcgacg cgctctccgg gggccgggtc atcgccggca tcggcgtcgg
     2881 ctggagcgag ccggagttcg cgaacctcgc gatggcggac cggttcgccg tccgcggcgc
     2941 atacctcgag gagacgatcg agctgtggcg ccatctgtgg tccggctccg gcgagccgtt
     3001 cgcggggcgc ttccacacgg tctccgacta ccgattcgga ccgctgccag cgcagggcgt
     3061 cggcctcccg atctggatcg gcggccgggc cgacgccgcc ctggagcggg ccgggcggct
     3121 ggccgacggg tatcactcct cggcctcggg gcccgagtcg ttcgcccgcc gggtcccggt
     3181 catccgcgcc gcggccgacg ccgcgggccg tccaatgccg acgctgtcgg cccgggtccg
     3241 ggtcgcgttc gacgagccgg tcgattccgc cgagccgcgg ccctacgcga tgcgcgggtc
     3301 ggccgaggac gtcgcggccg aggtggcgaa gttcgcggcg ctcggcgtcg agcacctcgc
     3361 gatcttcttc gaggccgaca cgatcgaggc gttcctcgcc gacgccgagc gcttcactgc
     3421 cgaggtcgcg ccgctcggct cgtaacgccc gcaggctagt ccggcgggag gtggagcggg
     3481 tcctcgcgcg acctcccaca gcccggcatc gcgcagcgtg cgccctgcgc gcggacgctg
     3541 gcatcggtca ccgcgatcga ctgtgccatc ccggttccgg gggtgcgtcc cgcgctcagt
     3601 gcgaggccga gccgattgtc ttccggctcg acgaaccgat gcggtccgct cgtgccgccg
     3661 aggcgctccg tgtttcgacc ggatctggac atcagcgatc ccccatgcgt gccacgacgg
     3721 tggac
//
LOCUS       DATAIW010000508         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_236526, whole genome shotgun sequence.
ACCESSION   DATAIW010000508 DATAIW010000000
VERSION     DATAIW010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236526"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VHM48_14965"
     CDS             complement(<1..794)
                     /locus_tag="VHM48_14965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756769.1"
                     /translation="MTAVLEAHGFGKRYRRKNWALRGVDLEVPEGSITALVGPNGSGK
                     STLIRAWIGFERATEGRLSTGGIDPRKDRAAAVAKVGYVPQSPSLYGNLTIAEHITLA
                     TTLRDGFDASLAAHYIVRLSIPLTAIASELSGGEQAQVGLALALGTRAPILLLDEPLA
                     SLDPLARREFLHVLVEAVRSEGHTALLSSHVITDIEQACDRLIVLGKGHTLLDLSISE
                     ALAHHRVIEGTPLVPVAPDRVVGSFPSAAGEGLTLIRVPVAQPLAA"
     gene            1026..2708
                     /locus_tag="VHM48_14970"
     CDS             1026..2708
                     /locus_tag="VHM48_14970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756770.1"
                     /translation="MSAAVVRDRFALLVAAIAFMAGLLPLTILRYDPGITLGLDSTSE
                     HLIVQAVDPLGPAAQDGVHPGQIVVAINGNQLIDLPQPTYSKPDLNGEQTITGADPAT
                     PRNRLPANGLAGLLNWSIDSLDLIEPGNLATGSAANGFVLAGVFYPGWHGLSDATQLI
                     LGGLAILLFGAWWIATGRAGDSIRDLAFPLVTAVSAPFFVSALSASGLPVAMTVAGIL
                     LVLAMLPLAGSLASRIDSPVDRRFAHIAIAVFASAAAIVAIAYVTQPLNRETLVQLRW
                     ALTGAIPIVPGLIATGPLDFRGGRTSGAGKVLRAAELAIAGATPLVALLVLGGLGIWP
                     LALWLVGIAAAGRYTVRPLARLATRATLQRDLVVAATEAERARLAADIHDDALQELTL
                     LVRRLEASGDTEGAEIGRTVADRLRAICGDLRLPILDDLGVGPALDWLVLRIERLAGG
                     EVRLERSDDARHNPDVELAIFRVAQEALANAVKHGKPPIVVRYRSTPGGISLAIDDAG
                     PGISDQAPADAEKAGRFGLLNMAQRAEQIGAILDVKRWPTGGTHVALEWRPR"
     gene            2705..3343
                     /locus_tag="VHM48_14975"
     CDS             2705..3343
                     /locus_tag="VHM48_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756771.1"
                     /translation="MSDAIRLLIVDDHPVVRDGTAALLDAQPGIAVAGTAGSIDEAIA
                     LIATVPADVLLLDIRLGTDSGLRLLTEPPGRLPAGAEEPPAIILLTAYDYPQYAEAAL
                     RLGASGFVLKTAPLAELLDAIRRVAAGGMAFSIRPRAQGGLARLSQRELDVVRLVVDG
                     RSNDEIGARLGIGPKTVESHLRRLFERFDLASRTELATRALREGWLEVPPGT"
BASE COUNT          467 a         1319 c         1244 g          517 t
ORIGIN      
        1 tcggcggcga gtggctgagc gacgggcacc cggatgagcg tgagcccctc gcccgcggcg
       61 gacgggaacg acccgacgac gcggtcgggt gcgaccggga cgagcggcgt gccctcgatc
      121 acccggtgat gggcgagcgc ctcggagatc gacaggtcga ggagggtgtg gcccttgccg
      181 aggacgatca gccgatcgca cgcctgctcg atgtcggtta tgacgtgcga cgacaggagg
      241 gccgtgtgcc cctcggatcg gacggcctcg acgaggacgt ggaggaactc gcggcgagcc
      301 aacggatcga ggctggccag tggctcatcg aggagcagga tcggcgcccg cgtgcccagc
      361 gcgagggcga gcccgacctg ggcctgctcg ccgccggaca gctcggacgc tatcgccgtg
      421 agggggatcg agaggcgcac gatgtagtgg gccgccaagc tcgcgtcgaa gccgtcgcgg
      481 agtgtcgtcg cgagggtgat gtgctcggcg atcgtcaggt tgccgtagag cgacggcgac
      541 tggggcacgt agccgacctt cgccactgcg gcggcccgat cctttcgggg gtcgatgccg
      601 ccggtcgaca ggcggccctc ggtcgcccgc tcgaacccga tccaggcccg gatcagcgtc
      661 gacttgccgg agccgttggg gccgacgagg gcggtgatgc tgccctcggg cacctcgaga
      721 tcgacgccgc gcagcgccca gttcttccgt cgatagcgct tgccgaagcc atgggcctcg
      781 aggaccgccg tcatccgtcc ctcctcggtt ggcgccggct ggaccggcgg tgcctgatga
      841 ctgtacccgg gcagcgtcgg ggaagcgtgc cggcgcccac cccccagagc gagcgccggc
      901 agcacgtgat gtgccgcggt catcgtcccg tgccgccacc gggccgccca gccgtcactc
      961 cccggggagc cgttgcggga caacccggta cggcccgtcc atgcgcctct gctaccgtcc
     1021 gaggcatgtc ggcagcagtc gttcgcgacc ggttcgcgct cctcgtcgcg gcgatcgcct
     1081 tcatggctgg cctcctgccg ctcaccatcc tccgatacga ccccgggatc accctcggcc
     1141 tcgactcgac ctccgagcac ctcatcgtcc aggccgtcga cccgctcggg ccggcggctc
     1201 aggacggcgt tcaccccggg cagatcgtcg tcgcgatcaa tggcaatcag ctgatcgacc
     1261 ttcctcagcc gacctattcc aagcccgatc tgaacggcga gcagacgatc accggcgccg
     1321 accccgcgac gccacgcaac cggctgccgg cgaacggcct cgcgggcctc ctcaactggt
     1381 cgatcgacag cctggacctc atcgagcccg gaaacctcgc gaccggcagc gccgcgaatg
     1441 gcttcgtgct cgccggggtc ttctatccgg gctggcacgg cctcagcgac gcgacgcagc
     1501 tgatcctcgg tggactcgcg atcctgctct tcggcgcgtg gtggatcgcg acgggacgcg
     1561 ccggagactc gattcgggac ctggcgttcc cgctcgttac tgccgtgtct gcgcccttct
     1621 tcgtgtcggc gctctccgcc tcgggccttc cggtcgcgat gaccgtcgcg ggcatcctcc
     1681 tcgtgctcgc catgctgccg ctcgccggca gtctcgcaag tcggatcgac tcgcccgtcg
     1741 atcgccggtt cgcgcacatc gcgatcgcgg tcttcgcgtc cgcagcagcg atcgtcgcga
     1801 tcgcgtacgt cacccaaccc ctgaatcggg agacgctcgt gcagctccgc tgggcgctca
     1861 cgggcgcgat cccgatcgtg cccgggctga tcgcgaccgg tccattggac tttcgcggcg
     1921 gccggacgtc gggagccggc aaggtgctgc gcgcagcgga gctggcgatc gcgggggcga
     1981 cgcccctcgt cgccctcctc gtccttggcg gcctcgggat ctggccgctg gcgctgtggc
     2041 tcgtcgggat cgcagcggcc gggcggtaca cggtccgtcc gctcgcccgg ctcgcgaccc
     2101 gggcgaccct ccagcgcgac ctcgtcgtcg cggccacgga ggccgagcgg gcccggctcg
     2161 cggcggacat ccacgacgac gccctccagg agctgaccct gctggtccgc cggctcgagg
     2221 cctctggcga cacggagggc gccgagatcg ggcggacggt ggccgaccgg ctgcgggcga
     2281 tctgcggcga cctccgcctg ccgatcctcg acgacctcgg ggtcgggccg gcgctcgatt
     2341 ggctcgttct ccggatcgag cggctcgccg gcggcgaggt acgcctcgag cggagcgacg
     2401 acgcccgcca caaccccgac gtcgagctgg cgatcttccg ggtcgcccag gaagcacttg
     2461 cgaacgcggt caagcacggc aagccaccga tcgtcgtccg ctaccgctcg accccgggcg
     2521 ggatctcgct cgcgatcgac gacgccggcc ccgggatcag cgaccaggcg ccggccgacg
     2581 ccgagaaggc cggccggttc gggttgctga acatggccca gcgggcggag cagatcgggg
     2641 cgatcctcga cgtcaagcgc tggccgaccg gcgggaccca cgtcgcgctc gagtggcggc
     2701 cgcgttgagc gacgcgatcc ggctgctcat cgtcgacgac cacccggtcg tccgggacgg
     2761 gacggccgcc ctgctcgatg cccagcccgg gatcgcggtc gcggggacgg ccggctcgat
     2821 cgacgaggcg atcgccctga tcgccactgt cccggccgac gtcctcctcc tcgacatccg
     2881 attgggcacc gattccgggc tccgcctcct gaccgagccg ccgggccgcc tgcccgccgg
     2941 cgccgaggaa ccgcccgcga tcatcctcct gaccgcctac gactatccgc agtacgccga
     3001 ggccgcgctc cggcttgggg cctcggggtt cgtcctgaag acggcgccgc tcgccgagct
     3061 cctcgacgcg atccgccggg tcgccgcggg cggaatggcg ttctcgatcc gcccgcgcgc
     3121 ccagggaggc ctcgcccgcc tcagccagcg cgagctcgac gtcgtccggc tcgtcgtcga
     3181 cggccgctcg aacgacgaga tcggcgcccg gctcgggatc ggcccgaaga cggtcgagtc
     3241 gcacctccgt cggctgttcg agcgattcga cctcgcgtcg cggacggagc tcgcgacgcg
     3301 cgccctgcgc gagggctggc tcgaggtgcc gcccggcacg tgaaccgggc aaacgaccga
     3361 cgtctggtgg cggacgaatc cggatgacgt tcgccgcttc acggacgaga acacgctacg
     3421 ctcggtccgt ggcccactcg cactctgcgg ccggtcgcca tgccggagct ctcgcggcag
     3481 cgatcgggat cggcgtcgtc gtcctcgccc tcgagctgat cgccgggatc gcggccaact
     3541 cgctcgc
//
LOCUS       DATAIW010000509         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_207814, whole genome shotgun sequence.
ACCESSION   DATAIW010000509 DATAIW010000000
VERSION     DATAIW010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207814"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VHM48_14980"
     CDS             complement(<1..360)
                     /locus_tag="VHM48_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2756772.1"
                     /translation="MSEPDRERAVHGTILALGRWVLLEACREAAAWRERLTDRLVLSV
                     NLSAAQLQQVDFVDDLRAILDETGFPAEDLVLELTETAMFHDTQTTIARLEALRALGI
                     RSAIDDFGTGYSSLGYLR"
     gene            complement(467..994)
                     /locus_tag="VHM48_14985"
     CDS             complement(467..994)
                     /locus_tag="VHM48_14985"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX2756773.1"
                     /translation="MFILYAIPIGVLGGFLAGGRLEHLAAVRFRLAPVAMIALVIQLA
                     LFSALADGLPTELVRGTYVLSTALVIVVVLANIRLTGVPLIVLGAACNLAAVMANGGA
                     MPASPAALDALGLGVGGHTSSIAVAHPALEPLTDVFAMPDWMPLANIFSVGDILIGLG
                     VALAIAAAMRGTSRA"
     gene            1152..>2546
                     /gene="leuC"
                     /locus_tag="VHM48_14990"
     CDS             1152..>2546
                     /gene="leuC"
                     /locus_tag="VHM48_14990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX2756774.1"
                     /translation="MTRPRTIVEKIWDDHVVGQDEGAPAILAVDLHLVHEVTSPQAFS
                     GLRARHLGVRHPERTVATADHSIPTHPRSLPMADPMAAAQVDQLTRNCTEFGIPLHGM
                     GDPGQGIVHVIGPQLGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEMVLATQTL
                     LQRQPKTYEVRVDGRLSAGVSSKDVILALIARIGIGGGTGHVFEYTGDAIRAMTMEQR
                     MTICNMSIEGGAKAGLIAPDDTTFEYVAGRRHAPSGAAWDAAVERWRTLPSDDGAVYD
                     KSITIDASALEPMVTYGTNPGMGIPITARVPAPGDMADGSARRALERALEYMDLQPGQ
                     AILGQKVDVVFVGSCTNGRISDLRLAAEVLRGWTVADGVRMMIVPGSDEVKREAEREG
                     LHEVFRAAGAEWREAGCSMCIAMNGDQLSPGQYAISTSNRNFEGRQGKGGRTFLASPL
                     TAAASAIAGVVADPR"
BASE COUNT          413 a          883 c          915 g          335 t
ORIGIN      
        1 gcgcaggtag ccgagggacg aatacccggt tccgaagtcg tcgatcgcgc tgcggatgcc
       61 gagggcccgg agcgcctcga gccgcgcgat cgtcgtctgg gtgtcgtgga acatcgcggt
      121 ctcggtgagc tccagcacga ggtcctcggc ggggaagccg gtctcgtcga ggatcgcccg
      181 aaggtcgtcg acgaaatcga cctgctggag ctgcgccgcc gacaggttga ccgacaggac
      241 cagccgatcc gtgaggcgct cgcgccaggc ggcggcctcg cgacaggcct cgaggagcac
      301 ccagcgaccg agggcgagga tcgttccatg gaccgcgcgc tcgcggtccg gctccgacat
      361 ggtgcctcca gctattcacc ccgtagtgca tgttgtacgt gggcaccgag ccggccggtc
      421 gtgggtccga agtcccgtgg ggagcatgac catcgccggg ggtgggctag gctcggctcg
      481 tcccgcgcat cgccgccgcg atggcgagtg cgacgcccag cccgatcagg atgtcgccga
      541 cgctgaagat gttggcgagc ggcatccagt cgggcatcgc gaacacgtcg gtcaggggct
      601 cgagagccgg atgggcgacg gcgatgctgc tcgtatggcc gccgaccccg aggcccaggg
      661 cgtcgagcgc cgcgggactg gccggcatcg cgcctccgtt ggccatgacg gcggccaggt
      721 tgcacgcagc gccgaggacg atcaggggta cgccggtcag tcgaatgttg gccaggacga
      781 cgacgatcac gagggccgtc gacagcacgt acgtcccccg gacgagctcc gtcgggagac
      841 cgtccgcgag ggccgagaac agggccagct ggatgacgag ggcgatcatg gcgacgggcg
      901 ccagccggaa ccggacggcc gcgaggtgct cgagacgccc gccggccagg aagccaccaa
      961 ggacgccgat cgggatggcg tagaggatga acacggggga agtatcgcgg tcggcgagcg
     1021 ccgggtttct cgcttcggcg gcacccgatc gtcgcccgcc gaggctgaac gaggcagggt
     1081 cgcaaccgcg ccgggcggcc ggctgcgcta ccctctgcca tccagccagc acacgagcga
     1141 gccgtcactc catgacccga ccgcgcacga tcgtcgagaa gatctgggac gaccacgtcg
     1201 tcggccagga cgagggcgcc ccggcgatcc tcgcggtcga cctccatctc gtccacgagg
     1261 tcacgtcccc ccaggcgttc agcggccttc gagcccgcca tctcggcgtc cgccatccgg
     1321 agcggacggt cgcgaccgcc gaccactcga tcccgaccca tccgcggagc ctgcccatgg
     1381 cggatccgat ggccgcggcc caggtcgacc agctgacccg caactgcacg gagttcggca
     1441 tcccgctcca tggcatgggc gaccccggtc agggcatcgt ccacgtcatc gggccgcagc
     1501 ttgggctgac ccagccgggg atgacgatcg tctgcggcga ctcgcacacg gcaacccacg
     1561 gcgcgttcgg ggccctcgcc ttcgggatcg gcacgagcga ggtcgagatg gtcctcgcga
     1621 cccagaccct cctccagcgc cagccgaaga cctacgaggt ccgggtcgac ggccgcctgt
     1681 ccgccggagt cagcagcaag gacgtcatcc tcgccctgat cgcccggatc gggatcggcg
     1741 gcgggacggg ccatgtcttc gagtacaccg gcgacgccat ccgggcaatg acgatggaac
     1801 agcggatgac gatctgcaac atgtcgatcg agggcggcgc aaaggccggc ctgatcgcgc
     1861 cggacgacac gacgttcgag tacgtcgccg gccggcggca cgccccgagc ggtgcggcat
     1921 gggacgcggc cgtcgagcgc tggcggacgt tgccgagcga cgatggcgcc gtgtacgaca
     1981 agtcgatcac gatcgacgcc tcggcgctcg agccgatggt cacctatggc accaaccccg
     2041 ggatggggat cccgatcacg gcgcgcgttc cggccccggg cgacatggcc gacggatcgg
     2101 ccaggcgggc gctcgagcgg gcgctcgagt acatggacct ccagcccggc caagccatcc
     2161 tcggccagaa ggtcgacgtg gtcttcgtcg gctcgtgcac gaacgggcgg atcagcgacc
     2221 tccgtctcgc ggccgaggtc ctccgcggct ggacggtcgc cgacggcgtc cggatgatga
     2281 tcgtgcccgg ctccgacgag gtgaagcgcg aggccgaacg cgagggcctc cacgaggtct
     2341 tccgggccgc cggcgccgag tggcgcgagg cgggatgctc gatgtgtatc gcgatgaacg
     2401 gcgatcagct gtcgcccggc cagtacgcga tcagcacctc gaaccgaaac ttcgagggtc
     2461 gccagggcaa gggcggacgg accttcctcg cctcgcccct gactgccgcc gcgagtgcca
     2521 tcgcaggggt tgtcgccgac ccccgt
//
LOCUS       DATAIW010000510         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_259522, whole genome shotgun sequence.
ACCESSION   DATAIW010000510 DATAIW010000000
VERSION     DATAIW010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259522"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VHM48_14995"
     CDS             <1..1255
                     /locus_tag="VHM48_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2756775.1"
                     /translation="VLRVCRAIKPLLDQVPPDIFSNDPEELVALASLGSRFRSMDKKV
                     LHDAVRLLTGSAADFLDDYFESEILKGYFASSAIIGTKVGPYSQGSGLVLLYHILGEH
                     DGEFGAWAFHKQGNGGFTKVLARAAQAFGAEIALESPVEKVITRDGRATGVALVDGTE
                     FHAPVVVSALDPRRTFLELVDPRELPTDLVDAIERFRFQGTSAKVNFALDGAPRYPAL
                     GDRTDQYRGFTNIGPSMEYLERAYDDAKYGWYSKRPYIDTAIQSTIDPDMAPPGKHVM
                     SCFVQYAPYHLRESDWDTEKERFGDTVQATLESFFPGFGDLVLQREIQTPLDIERTVG
                     LSEGNIFAGEFLAPQMFLFRPAPGWSQYRTPIDGYYQCGSGTHPGGCVMGAPGKLAAG
                     QILKDRARTGQEVRDPVAVDAAAGA"
     gene            complement(1290..2324)
                     /locus_tag="VHM48_15000"
     CDS             complement(1290..2324)
                     /locus_tag="VHM48_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756776.1"
                     /translation="MTDQPPLAAALANARAAAPPGVEIRPWRAQGDFTAMVEVFHHAR
                     VVDGTSWDLSVEGLVADIRGMGLRPEDSILLAVVDGQVVGWTRTWDFGRSPDEGRLLM
                     HSGQVVPAVRGRGIGRALLRGAQAELERIRAAAADPTGTTAGLHSWLFARNTSAIALL
                     EAAGYRQLRFVVEMTRPLDDLPTVDLPAGLATRPVRPEDRPAIARAMDEAMHDHRGWP
                     DWSDEQLMSMLDHPNRGQLDVWQVAWHGDRVVGGVLGYIDDHENAVMDRRRGYTEGIF
                     TVRDWRGRGVAGALIARNLHLLRERGMVEAALSVDTENPSGALGLYQQHGFREHDRLI
                     IFRKDLAPAA"
     gene            2714..3007
                     /locus_tag="VHM48_15005"
     CDS             2714..3007
                     /locus_tag="VHM48_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756777.1"
                     /translation="MRALTIGTSLASIAAVGGFGAMAALSYDGTSSNVTTAAVVTADN
                     GATTTGTDASTTTGTATTTDTTTTTTTSGTTSIQATAAPATTTGTAHAATGSS"
     gene            3004..4005
                     /locus_tag="VHM48_15010"
     CDS             3004..4005
                     /locus_tag="VHM48_15010"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX2756778.1"
                     /translation="MTEPPTADWRALGTGVRLVVLDGDLDAARRAVEDVLDDVDRAYS
                     RFRDDSEIVALNARAGSPVAVSPLLATAIAAGLRAARITDGAVDPTVGRAMRAIGYDA
                     DFDRIRNPAGPIELRIEPIPGWQSVEFSAPRREVRVRRGVELDLGSTGKGLASDLAAA
                     AALAAIAVPGEQARGGVLVSLGGDIAVGGTPPDGGWRILIAEDSETPSDAAGEVVAIE
                     TGAMATSSTTVRRWQRGARSLHHLIDPRTGGPVESPWRTASVVAATCVDANTAATAAI
                     VMGDAAIAWLEAARLPARLVGLDGTVTRLGGWPEPDDDPGADHIDAATDESGRRPTA"
     gene            4091..4693
                     /locus_tag="VHM48_15015"
     CDS             4091..4693
                     /locus_tag="VHM48_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX2756779.1"
                     /translation="MSTQILWFTTRGAGVVSLLLFSAVACLGLLAVARTQSVRWPRFL
                     TVELHRNLALLSVVFLGIHIVTAILDPFTSLGIGAALVPLASSYRPLPVAFGVVSVYL
                     VLAIIITSLLRERIGHRVWRAVHWSSYAAWPLAIEHTLTAGSDSFSVWLLVVQAACVL
                     AVSGALVWRLTSGGTNRSRLAAVTGGTADPIAVDPAWRNH"
     gene            4693..>5038
                     /locus_tag="VHM48_15020"
     CDS             4693..>5038
                     /locus_tag="VHM48_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756780.1"
                     /translation="MEPRLFAGPPAASGPETHADHLARLGRLPSGSSAPAVIPVLEAS
                     GLLGRGGAGFPVGRKWRSVAGQTGGRPVVLVNGAEGEPLSAKDRTLLRLRPHLVLDGA
                     LLAADAVGADRIA"
BASE COUNT          718 a         1872 c         1725 g          723 t
ORIGIN      
        1 cgtgctgcgg gtctgccggg cgatcaagcc gctcctcgac caggtcccgc ccgatatctt
       61 cagcaacgac ccggaggagc tcgtcgccct cgcgtccctc ggctcgcgct tccgatcgat
      121 ggacaagaag gtcctccacg atgcggtccg gctcctgacc ggctcggccg ccgacttcct
      181 cgacgattac ttcgagtccg agatcctcaa gggctatttc gcgtcttcgg cgatcatcgg
      241 cacgaaggtc gggccgtatt cgcagggatc cgggctcgtc ctgctgtacc acatcctcgg
      301 tgagcacgac ggcgagttcg gggcgtgggc cttccacaag cagggcaacg gcgggttcac
      361 caaggtcctc gcccgggcag cccaggcgtt cggtgccgag atcgccctcg agtcgccggt
      421 cgagaaggtc atcaccaggg acggccgggc gaccggcgtc gccctggtcg acgggaccga
      481 gttccacgct cccgtggtcg tctccgcgct cgacccgcgg cggacgttcc tcgagctcgt
      541 cgacccgcgc gagcttccga cggacctggt cgacgcgatc gagcggttcc gcttccaggg
      601 cacgtcggcc aaggtcaact tcgccctcga cggcgctccg cgctaccccg ccctcggcga
      661 ccggacggac cagtaccgcg gattcacgaa catcgggccg tcgatggagt acctcgagcg
      721 ggcctacgac gatgccaagt acggctggta ctcgaagcgg ccctacatcg acaccgcgat
      781 ccagtcgacg atcgacccgg acatggcacc acccggcaag cacgtgatga gctgcttcgt
      841 ccagtacgcg ccgtatcacc tccgcgagag cgattgggac accgagaagg agcgcttcgg
      901 cgacacagtc caggcgacgc tcgagtcgtt cttcccgggc tttggcgacc tggtcctcca
      961 gcgcgagatc cagacaccgc tcgacatcga gcggaccgtc ggcctgtcgg agggcaacat
     1021 cttcgccggc gagttcctcg ctccccagat gttcctgttc cgcccggcgc ccggctggtc
     1081 gcagtaccgg acgcccatcg acggctacta ccagtgcggc tccgggacgc accccggcgg
     1141 gtgcgtgatg ggggcgccgg gcaagctcgc cgcgggccag atcctcaagg accgcgcacg
     1201 gaccggccag gaggtgcgcg acccggtcgc cgtcgacgct gccgccggcg cctgacaacc
     1261 gggatccgcc cggcctcgcg gcgcccggct caggcggccg gcgcgagatc cttgcggaag
     1321 atgatcagcc ggtcgtgctc ccggaagcca tgctgctggt acaggccaag cgccccgctc
     1381 gggttctcgg tgtcgaccga cagggcggcc tcgaccatgc ctcgctcccg cagaaggtgc
     1441 aggttgcgcg cgatgagggc gccggcgacg ccgcgaccgc gccagtcgcg aacggtgaag
     1501 atcccctcgg tgtagccgcg ccgccgatcc atgaccgcgt tctcgtggtc gtcgatgtag
     1561 ccgaggacgc cgccgacgac gcggtcgccg tgccaggcga cctgccagac gtcgagctgg
     1621 ccccggttcg gatggtcgag catgctcatc agctgctcgt ccgaccagtc cggccagccg
     1681 cgatggtcgt gcatcgcctc gtccatcgcc cgagcgatgg ccggtcgatc ctcggggcga
     1741 acggggcgcg tcgcaaggcc ggccggcaga tcgacggtcg ggagatcgtc gagcggtcgc
     1801 gtcatctcga cgacgaaccg cagttgacga taccccgcgg cctcgaggag ggcgatcgcc
     1861 gaggtgttcc gcgcgaacag ccacgaatgc aggccggcgg tggttcccgt cgggtcggct
     1921 gccgcggccc ggatgcgttc gagctctgcc tgtgctccgc gcagcagggc ccgtccgatg
     1981 ccgcgaccac gcaccgcggg cacgacctgt ccggagtgca tcaggaggcg gccctcatcg
     2041 ggagaccggc cgaaatccca ggtgcgggtc cagccgacca cctgcccatc gacgacggcc
     2101 agcaggatcg aatcctcggg tcgtagcccc atcccacgga tgtccgcgac gagcccctcg
     2161 accgagagat cccagctggt gccatcgacc acccgggcgt gatggaacac ctcgaccatc
     2221 gcggtgaagt cgccctgggc gcgccacgga cggatctcga caccgggcgg cgcggcggcc
     2281 cgagcgttgg cgagtgcggc agcgaggggc ggctggtcgg tcatcagcac cgagtgcgag
     2341 gcctcctggg atcgtttcgg gatggcgcgc cgggatcgtg gacaccgtgc catcgaccat
     2401 cggtcaaagt tgtcgcgaac gagacggccg gagtgccgat tgcgggacag aaccgtcgcc
     2461 gtggcggcag ccgcccggcc ggtcgggtgc ggccgctgcc gggctcaggt tgctggtgag
     2521 gcgcgggtcg cgccgcggtc acggctcgga gtcagtcgac gaccatcccg aacggagcgc
     2581 cgccgcccgg ggatcgcgat gcgacggttc gctgaccttc gtcatccttc ggcaaggaac
     2641 ggcgggtcat cgtgtggtca tgaagacagc aacccgacgc agcccagccg accgagaccg
     2701 agcggtcgcc cgcttgcggg ccctcacgat cggaaccagc cttgccagca tcgccgcggt
     2761 cggcgggttc ggcgccatgg ccgccctctc ctacgacggc acctccagca acgtcaccac
     2821 ggccgcggtc gtcaccgccg acaatggggc aacgacgacc ggcacggacg cgtcgaccac
     2881 gacgggcacg gcaacgacga cggacacgac gacgaccacc acgacgagcg gcacgacctc
     2941 catccaggcc acggccgcac cggccacgac gaccgggacc gctcacgccg ctaccggatc
     3001 ctcgtgaccg agccaccgac cgccgactgg cgggccctcg gaaccggcgt ccggctcgtc
     3061 gtcctcgacg gcgacctgga cgccgctcgg cgcgccgtcg aagacgtcct cgatgacgtc
     3121 gatcgggcat acagccggtt ccgcgacgac agcgagatcg tggccctcaa tgcccgcgcc
     3181 ggatcaccgg tcgcggtcag cccgctcctg gcgacggcga tcgccgccgg actgcgggcc
     3241 gcccggatca ccgacggcgc ggtcgacccg accgttggcc gggcgatgcg ggccatcggc
     3301 tacgacgccg atttcgatcg gatccgcaac ccggcggggc ccatcgagct ccggatcgag
     3361 ccgatccccg gctggcagtc ggtcgagttc tccgcgcccc gccgcgaggt ccgggtccgc
     3421 cgcggcgtcg agctcgacct cggctcgacc ggcaagggac ttgcctcgga cctggccgcg
     3481 gccgccgccc tcgcggccat tgccgtgccc ggggagcagg cccgtggtgg cgtgctcgtc
     3541 tccctcggcg gcgacatcgc cgtcggcgga accccgcccg acggcggctg gcggatcctg
     3601 atcgccgagg acagcgaaac cccgtccgac gccgcgggcg aagtggtggc gatcgagacc
     3661 ggcgcgatgg cgacctcgag cacgaccgtc cggcgctggc agcgcggcgc acggagcctc
     3721 catcacctga tcgatccgcg gaccggcggg ccggtcgaat cgccgtggcg gaccgcgtcg
     3781 gtcgtcgccg cgacgtgcgt cgacgccaac acggcggcca ccgcggccat cgtcatgggc
     3841 gatgcggcga tcgcctggct cgaagccgct cgcctgccgg ccaggctcgt cggtctcgac
     3901 gggacggtga cccgactcgg cggctggccg gagcccgatg acgatcccgg cgcggaccac
     3961 atcgacgcgg caaccgacga gtccgggcga cgtcccaccg cgtaactcga tcgaaaggtg
     4021 cggactctca tgatcggctc atcccgatcg gggacatgat ctcgaactca tttgccggag
     4081 cgcccaaccc gtgagcaccc agatcctgtg gttcaccacc cggggagccg gcgtcgtctc
     4141 cctcctcctg ttcagcgctg tcgcctgcct cggcctgctg gccgtcgccc ggacccagtc
     4201 cgtgcggtgg ccgcggttcc tgacggtcga gctccaccgc aacctggcgc tcctgtcggt
     4261 cgtgttcctc gggatccaca tcgtcaccgc gatcctcgac ccgttcacga gcctcggcat
     4321 cggcgcggcg ctcgtgccgc tggcgtcgtc ctaccggccc cttccggtgg ccttcggtgt
     4381 cgtgtcggtg tacctcgtcc tcgcgatcat catcacgagc ctccttcgcg agcggatcgg
     4441 ccaccgggtc tggcgggccg tccactggtc gtcctacgcg gcctggccac tggcgatcga
     4501 gcacaccctg accgccggca gcgactcctt ctccgtgtgg ctcctcgtcg ttcaggcggc
     4561 gtgcgtcctt gccgtcagcg gcgcgctggt ctggcggctg acctcgggcg gaaccaaccg
     4621 cagccggctt gcggcagtga ccggcggcac ggccgatccg atcgccgtcg acccggcctg
     4681 gaggaaccac tgatggagcc gcgcctgttc gccgggccgc ccgccgcgtc aggccccgag
     4741 acccatgccg atcacctcgc ccggctcggg cggctgccat ccggatcgtc ggcaccagcc
     4801 gtgatcccgg tcctcgaggc gagcggcctg ctcggtcgcg gcggcgccgg gttcccggtc
     4861 ggccggaaat ggcgctcggt cgccgggcag accggtggcc ggccggtcgt cctcgtcaac
     4921 ggcgccgagg gtgagccgct cagcgccaag gaccggacct tgctccggct ccggccgcac
     4981 ctcgtcctcg acggtgccct gctggcggcg gacgctgtcg gcgccgatcg gatcgccc
//
LOCUS       DATAIW010000511         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_140672, whole genome shotgun sequence.
ACCESSION   DATAIW010000511 DATAIW010000000
VERSION     DATAIW010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140672"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VHM48_15025"
     CDS             complement(<1..1091)
                     /locus_tag="VHM48_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2756781.1"
                     /translation="MPFASPLALLGLLFVPAVVAMYLLRLRRTETIVPSTLLWQRLAA
                     DVEANAPWQKLRRSLLFLLQLLLVAILAFLAARPFVERPAGLARDLVLIVDTSASMAA
                     TDVPPDRLGAAKRAAIEALKDLPSGGKVSVIEAGRTARIVATGTSDLGRVRQAIEGIR
                     PTVSRGDLGDALALAQQLAIQSGDAEVLVATDAAFAVPPTTKVDAPIRVLRVGDAKGS
                     RNQAIVALAVRTSPTLTRSVFVSVANLDLESATRRVEVWSDDRLLETRTITIDPQSRA
                     DVIIDDVPADAAILDVRLVAGDAADTTARPDLLATDDRAWAVIPPVRERNILLVGAGD
                     PYLETALSYLPNSRLFGLKPDEYPAGAVRT"
     gene            complement(1101..2126)
                     /locus_tag="VHM48_15030"
     CDS             complement(1101..2126)
                     /locus_tag="VHM48_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2756782.1"
                     /translation="MTMTDEHEPAPAEAAARPGALTAAAPSGTSAAGSIKGPNGRPVF
                     LPSDVDPTVFDEAFLRQLERLLLLMHAPVRGGLKGGRRSVKRGQSVEFADYREYALGD
                     DLRQLDWNVLARLEKLFVKLFIEEEDVTITFLLDGSASMASGRPDKLQFAKRAAAALG
                     YIGLASEDKVAVSVLGGRSARRRTALRGSGRVFRLLAELSSIAVADGPTDLVAAARHA
                     AAQLSGRGVVILISDLLDPAADRVIRELASTRSELIVLHVLSPEELDPPLEGDLRLVD
                     AETGESIDVTADLATIDAYRGRLAAWKESFAQLAARRRATYVDLSTEVPLNDLIFAEL
                     RRRRVLG"
BASE COUNT          330 a          758 c          876 g          301 t
ORIGIN      
        1 gtccggacgg cgcccgccgg gtactcgtcg ggcttcaggc cgaagaggcg gctgttcggg
       61 aggtaggaga gagccgtctc gaggtacggg tcgccggcgc ccacgaggag gatgttccgc
      121 tcgcggaccg gcggaatgac ggcccacgct cgatcgtcgg tcgcgaggag gtccggccgg
      181 gcggtcgtgt cggcggcatc gccggcgacg agccggacgt cgaggatcgc ggcgtcggcc
      241 ggcacgtcgt cgatgatcac gtcggcccgc gactgggggt cgatcgtgat cgtccgggtc
      301 tcgagaagcc ggtcgtcgct ccagacctcg acccggcggg tcgccgactc caggtcgaga
      361 ttggcgacac tgacgaagac cgaccgggtg agggtcggtg aggtccggac cgcgagggcg
      421 acgatcgcct ggttccggct gcccttggcg tcgccgaccc ggaggacccg gatcggggcg
      481 tcgaccttcg tcgtcggcgg gaccgcgaag gcggcgtcgg tcgcgacgag gacctctgcg
      541 tcgcccgact ggatggcgag ctgctgggca agggccaggg catccccgag atcgccccgg
      601 ctgaccgtcg gccggatccc ctcgatggcc tgccgaaccc ggccgaggtc gctcgtcccg
      661 gtggccacga tccgggccgt ccggccggcc tcgatcacgc tgaccttgcc gccgctcggg
      721 aggtccttga gcgcctcgat cgctgcccgc ttggcggccc cgagccgatc cggtggcacg
      781 tcggtcgcgg ccatgctcgc cgaggtgtcg acgatcagga cgagatcgcg agcgagaccg
      841 gccggtcgct cgacgaacgg tcgagccgca aggaacgcga ggatcgcgac cagcagcagc
      901 tgcagaagga acaggaggct ccggcgcagc ttctgccacg gggcgttggc ctcgacgtcg
      961 gcggcgagcc gttgccagag gagcgtcgag ggcacgatcg tctcggtccg ccgcaggcgg
     1021 agcaggtaca tcgcgacaac ggccggcacg aacagcaggc cgagcagcgc cagcgggctc
     1081 gcgaagggca tcggatcagc tcagccgagg actcggcgcc ggcgcagctc ggcgaagatc
     1141 aggtcgttga gcggcacctc cgtcgagagg tcgacgtacg tcgcccggcg ccgggcggcg
     1201 agctgggcga acgactcctt ccaggcggcg aggcgtccgc gataggcgtc gatggtggcg
     1261 aggtcggcgg tgacgtcgat gctttcgccg gtctccgcgt cgaccagccg gaggtcgccc
     1321 tcgagcggcg ggtcgagctc ctcgggcgac aggacgtgga ggacgatgag ctccgagcgg
     1381 gtggaggcga gctcgcggat cactcgatcg gcggccgggt cgagcaggtc cgagatcagg
     1441 atcacgacgc cccggccgga cagctgggcc gccgcgtgac gggccgcggc gacgagatcg
     1501 gtcgggccgt cggcgaccgc gatcgacgac agctcggcga ggagccggaa gacgcgaccc
     1561 gagccgcgca gggcggtccg ccgtcgggcc gatcggccgc cgaggacgct gacggcgacc
     1621 ttgtcctccg acgccagccc gatgtagccg agggcggcgg cggcgcgctt ggcgaattgc
     1681 agcttgtcgg gtcggccgct cgccatcgac gccgagccgt cgaggaggaa cgtgatcgtc
     1741 acgtcctcct cctcgatgaa cagcttgacg aagagcttct cgagccgggc gaggacattc
     1801 cagtcgagct ggcggagatc gtcgccgaga gcgtactcgc gatagtcggc gaactcgacg
     1861 gactggccgc gcttgacgct ccgccggcca cccttcagcc cgccccggac gggggcgtgc
     1921 atcagcagga ggagccgctc gagctggcgc aggaatgcct cgtcgaagac cgtcggatcg
     1981 acgtcgctcg gcaggaagac gggccggccg ttcggtccct tgatcgagcc ggccgccgag
     2041 gtgccgctcg gagcggcagc cgtcagggct cccgggcggg cggcggcctc agccggagcc
     2101 ggctcgtgct cgtccgtcat ggtcatcgct gggtccggct cgccgggcgg actactcaac
     2161 cgccggcggg gcgaccgaat cgaggaccgt ccggatcgcc gcctcggtcg tgatcccttc
     2221 ggcctcgccc tcgaaattga ggatgatccg gtggcgcagc gaggg
//
LOCUS       DATAIW010000512         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_259961, whole genome shotgun sequence.
ACCESSION   DATAIW010000512 DATAIW010000000
VERSION     DATAIW010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259961"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /gene="glnQ"
                     /locus_tag="VHM48_15035"
     CDS             <1..255
                     /gene="glnQ"
                     /locus_tag="VHM48_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HEX2756783.1"
                     /translation="LFDEPTSALDPTLVGEVLKVMEGLAHEGATMIVVTHEMAFAREA
                     ADRVYFIDDGIFVEVAPHTELLDHPRDQRTRDFLARTLRT"
     gene            complement(149..1198)
                     /locus_tag="VHM48_15040"
     CDS             complement(149..1198)
                     /locus_tag="VHM48_15040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2756784.1"
                     /translation="MSSTASPAPSTRSRSTASRSAKRSSASFILLPSGASRTPRRRAP
                     GSPAPRPCELRTGAPIVAEVEGRIVGTGCGSAHGAAGWVGVIFVAPECRGNGLGRRIT
                     RAVLDDLEARGCRTQILIASPLGRPIYEREGFTVLERQVRFSIDGLPPDERPLDPDIR
                     PFAPDDLGATLALDACVTGEDRGAVLRTVLSPQTTYVATDPAGVVRGYLARAPWRGGA
                     LIAHEPGDALRLLEIRRHSTGASGKAGAGVLESNTTGRERLRAAGWHEEMGNVRMIPG
                     RAARVAAPRDLGPAQRRPRLRRSVEFVDRSGSIGPPDPVRFAGCGRGSRVCADRADGP
                     AARCAGRPRRRCRRR"
     gene            1113..1376
                     /locus_tag="VHM48_15045"
     CDS             1113..1376
                     /locus_tag="VHM48_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756785.1"
                     /translation="MNDAEERFAERLAVDLERVLGAGLAVDDIELSAAEDECSVRATL
                     LVEGRIETIEVSAPDIVALYRPLIERAAEIRLAGAFWRMVGPT"
     gene            1715..2290
                     /locus_tag="VHM48_15050"
     CDS             1715..2290
                     /locus_tag="VHM48_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2756786.1"
                     /translation="MEEDAIRPLSSEPSLAPVDSRSVVAARGDIVMRAFEEHAGKLTS
                     FAFGMTRDRDVADDLVQESFLRLVKELAADRTPDNIPAWLFRVCANLATSRGRRATVA
                     QRFLSRVHSVPDAAPADVETLRRETNMTLLAGLATVPSDARAALLMASQGFSGREIAE
                     AIGRSEMATRTMMFRAREKLRAYLVREEVRS"
     gene            2287..>3659
                     /locus_tag="VHM48_15055"
     CDS             2287..>3659
                     /locus_tag="VHM48_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756787.1"
                     /translation="MIHEHDDLVLASAAIDFDLTEDEADRLRRAVADCPICAERAAAY
                     RRQFQVLADLPQLEPSDATRRRVMAVAMSGRRAETRSPMLLLAAALVIGALLALVAAV
                     AGGAFKQRLIAEYPPVSESNSPVASAIAVASVEPGPGSSQPAGPGPGGGPTILGRDVI
                     ADVISSNLRLRSQPRIATDSIKFEPFLNVGDRLFVVDGPVVASNHDWYQVATWRPSDP
                     NATWPIGWVASADLDGTPWIAAAATTCPAAPTVDILAAMNRYEALACYGHRTIAFRAL
                     VSGSGPVDPCPGDLTTTCLGGPTWLAGTGGWTATVDASAVAAVPATAVLTFARDPAGP
                     VTENDLPTSRMLTISGAFDDPAAASCGLEGAPASVSTLTVTDAILRCRTTFVVSAVVP
                     DPGWLSIQVAAITTTPGLRVRSLPLVDDSSERFEPLLDQATRLFVIDGPVVGSGYDWY
                     RVVAPTVT"
BASE COUNT          520 a         1325 c         1293 g          521 t
ORIGIN      
        1 ctcttcgacg agccgacgtc ggcgctggat ccgacgctcg tcggcgaggt cctgaaggtc
       61 atggaggggc tggctcacga gggagccacg atgatcgtcg tgacccacga gatggcattc
      121 gcccgcgagg ccgccgaccg cgtctacttc atcgacgacg gcatcttcgt cgaggtcgcc
      181 ccgcacaccg agctgctgga ccatccgcgc gatcagcgca cacgcgactt cctcgcccgc
      241 accctgcgaa cctgaccggg tccggcggac cgatcgatcc ggaccgatcg acgaactcga
      301 ccgatcgcct cagccgaggg cgccgttgag ctggccccag atcgcgtggg gctgccactc
      361 gagcggctcg ccccgggatc atccggacgt tgcccatctc ctcgtgccag ccggcggccc
      421 gcagccgctc gcgtcccgtc gtgttgctct cgaggacgcc ggctccggcc ttgccgctcg
      481 ccccggtcga gtggcgccgg atctcgagca gccgcagcgc gtcgcccggc tcgtgggcga
      541 tgagcgcgcc gccccgccac ggggcgcggg cgaggtagcc gcgaacgacg ccggccggat
      601 cggtcgcgac atacgtggtc tgcggtgaga gcacggtccg gagcaccgcg ccgcggtcct
      661 cgccggtgac gcaggcgtcg agggcgaggg tggcgccgag gtcgtccggc gcgaacggcc
      721 ggatgtccgg atcgaggggg cgctcgtcgg gcggcaggcc gtcgatcgag aagcggacct
      781 ggcgctccag gacggtgaac ccctcccgct cgtagatcgg gcggccgagc ggcgacgcaa
      841 tgaggatctg ggtccggcag ccacgcgcct cgaggtcgtc gaggacggct cgcgtgatcc
      901 ggcgcccgag accgttgccg cggcactccg gcgcgacgaa gatcacgccg acccagccgg
      961 cagcgccatg ggccgagccg catcccgtcc cgacgattcg tccctcgacc tcggcgacga
     1021 tcggggcgcc ggttcgaagc tcgcaaggcc gaggagctgg ggatcccggt gctcgacgcc
     1081 gaggggttcg cgaagctcct gaggggagaa ggatgaacga tgcagaggag cgcttcgcgg
     1141 agcggctggc ggtcgacctc gagcgggtgc tcggcgccgg gctggcggtc gacgacatcg
     1201 agctgtcggc ggcagaggac gagtgcagcg tccgggccac gctcctggtc gaaggtcgga
     1261 tcgagacgat cgaggtcagt gcgcccgata tcgtcgccct ctaccggccg ctgatcgaac
     1321 gggcggccga gatccgcctg gccggcgcgt tctggcggat ggtcggaccg acctgacggg
     1381 tcgaatggga acgagtgatg tcgaaggtcg agacgagcac gccgccgaac acgccgacgc
     1441 cgatcggccc gtacagccac gtcgcccgcg tcggcccgcc atcggtcccg gcgagctgac
     1501 cggtggccgg gtcgacaccc gccgtcccac cgatcgtcac agggtcgcag atgcgccgga
     1561 ccgtgatccg ggtccatgaa ctgccgaaac cgggcgtcct cctgacgatg aacctgacgg
     1621 cggtcgcgag agactgacga cggcccgccc cggagcgcag aatcggggca gcacgaacgc
     1681 ggtaacaacc ttgcccgacg aacgtcatcg cgccgtggag gaggacgcca tccgacccct
     1741 gtcgagcgag ccgtcgctcg ccccggtcga ttcgcgatcg gtcgtcgcgg cccgtggcga
     1801 catcgtcatg cgcgccttcg aggagcatgc cgggaagctc acgagcttcg cgttcggcat
     1861 gacccgcgat cgcgacgtgg cggacgatct tgtccaggaa tccttcctcc gactcgtcaa
     1921 ggagctggcc gcggaccgca cccccgacaa catccccgcc tggctgttcc gggtctgcgc
     1981 caacctcgcc accagtcgag gccgccgggc gaccgtcgcc cagcggttcc tcagccgggt
     2041 ccacagcgtg ccggacgctg cgcctgccga tgtcgagacc ctccgccggg agacgaacat
     2101 gacacttctg gctgggttgg ccacggtccc gagcgacgcc cgggccgcgc tcctcatggc
     2161 ctcacagggg ttcagcggac gggagatcgc cgaggccatc ggccggagcg agatggcgac
     2221 ccggacgatg atgttccggg cccgggagaa gctccgggcc tacctcgtcc gggaagaggt
     2281 gcggtcgtga tccacgagca cgacgacctc gtgctggcat cggcggcgat cgacttcgac
     2341 ctgaccgagg acgaggcgga tcggctccga cgggcggttg cggactgtcc gatctgcgcg
     2401 gagcgcgccg cggcctaccg tcgccagttc caggtcctcg cggacctgcc gcagctcgag
     2461 ccgtcggacg cgacccggcg gcgggtcatg gccgtggcca tgtccggccg ccgggccgag
     2521 acgcgttccc cgatgctcct cctcgccgcc gcgctcgtga tcggtgccct cctcgccctg
     2581 gtcgcggcgg tggccggcgg cgccttcaag caacggttga tcgccgaata cccaccggtc
     2641 agcgagtcca actcgccggt cgcgtcggcg atcgcggtgg cgtcggtcga accgggtccc
     2701 gggtcgagcc agcccgccgg acccggacca ggcggcgggc cgacgatcct gggtcgggac
     2761 gtgatcgccg acgtgatctc gtccaacctg cgccttcgct cccagccacg gatcgcgacc
     2821 gactcgatca agttcgagcc gttcctcaat gtcggcgacc gtctgttcgt cgtcgacggc
     2881 ccggtcgtcg ccagcaacca cgattggtat caggtcgcga cctggcgacc ttcggatccg
     2941 aacgcgacct ggccgatcgg gtgggtcgcg tcggccgatc tcgatggaac gccgtggatc
     3001 gccgcggcgg ccacgacgtg cccggcagcg ccgaccgtcg acatcctcgc ggccatgaat
     3061 cgctacgagg ccctcgcctg ctacggccac cggacgatcg cgttccgggc cctggtgtcc
     3121 ggctccggtc cggtcgaccc gtgtccgggc gacctcacga ccacgtgcct cgggggtccg
     3181 acctggctcg ccgggacggg cggctggacg gcgacggttg acgcgagcgc cgtcgcagcg
     3241 gtcccggcga cggcggtcct cacgttcgcc cgggacccgg ccggcccggt cacggagaac
     3301 gacctgccga ccagtcggat gctcacgatc tccggcgcgt ttgacgaccc ggccgccgca
     3361 tcctgcggac tcgagggcgc gccggcgtcc gtgtcgaccc tgaccgtgac cgacgccatc
     3421 cttcgctgcc ggacgacctt cgtcgtgagc gccgtggttc cggaccctgg ttggctgagc
     3481 atccaggtcg ccgcgatcac gaccacgccg ggtctccgcg tccgatcgct gcccctggtc
     3541 gacgacagct cggagcggtt cgagccgctc ctcgaccagg ccacccgcct gttcgtcatc
     3601 gacggtcccg tggtcggcag cggctacgac tggtaccgcg tggtcgcgcc gacggtgac
//
LOCUS       DATAIW010000513         6490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_141608, whole genome shotgun sequence.
ACCESSION   DATAIW010000513 DATAIW010000000
VERSION     DATAIW010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141608"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..3050)
                     /locus_tag="VHM48_15060"
     CDS             complement(105..3050)
                     /locus_tag="VHM48_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2756788.1"
                     /translation="MGIAFDRPEVLLLLPILVGLTLIVHAAARHRLGIGRRRAALLVR
                     AILLGVLTFALAGFQLILPMDRLATVFVVDLSDSVGNAGREDGLAFLRQALAAMPRDD
                     LAGVVAFGRDALVERLPAEVRDIDRLASTPVRSATDIGAALRLASALFPDDAQRRIVL
                     LSDGNDTTGTGQDAAALAASRGIQIETRLLGGTATDEVLVERLTTPTTSRLGEEIEAT
                     ATITSTVAQPATVRLFADGGLVETQRVDLPAGTTAVTFRVKPTEPGFHVFRAVVEAAR
                     DTFDKNNRADSDTIVKGPPRTLVVAGDAKVAAELVAALTTERQTTELVAPDAVPSDLA
                     GLANYDSVVLVDVPRTDLGDRRMAALQSYVRDLGRGLVMIGGPHSYGAGGYTNTPLEE
                     VLPVDMGVRDKTKQPDIALVVVIDQSGSMDACHCNTFNQSGGTAIQGVRKVDIGKEAI
                     LRAVAALQATDSIGVVGFNEAAHWIVRTQPLGDVTSLQDQIAGIQPNGQTNIFAGLDA
                     AVGSLETATATRRHIILLTDGWSRSGAYDQILARMKAAGITLSTVGAGGGAAPVLAQL
                     AAQGGGRYYPAVNPSSIPDIFLKETQQVSGQQIVEEPFHPAQTASSPILRGLDAGYPP
                     LLGYNGTTAKAAAQTILVTGRDDPLLAQWQYGLGRSVAWTSDTTGRWAKDWLPWRDFS
                     RFASQLVAWTFPGEETGGIEARFDTRGEQTTLHVESVEADGSPRDFYATTAKIVGPDL
                     QPVDATLVQVAPGIYEAPLGALDPGAYAIRLSQTRPGTAPLGRTVGLVAPTATEYRRL
                     GTNETLLGALRSATGGTVVAAPSDVWRHDLSRTSHYADLWPLLLVIAMLLWPLDVALR
                     RVSLGRREFAAAWAWAAGWRHRRVAARPASLTGMLAARDRAGSAAARAAIRRDSSTTP
                     EAEAAAATPSREPRPARGSAASAALAAAPAPAAESAPVAATPSAAALDLDADTLERLR
                     AAKRRARGG"
     gene            complement(3050..5215)
                     /locus_tag="VHM48_15065"
     CDS             complement(3050..5215)
                     /locus_tag="VHM48_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2756789.1"
                     /translation="MPLIAPLALAGLAFIPLVVAFYLLKLRRDEHVVPSTLLWQRLVA
                     DVEANAPWQRLRRSLLLLLQLLLVAVLAVLAARPFVERPAGLARDIVLVIDTSASMGA
                     TDVFPNRLEAAKQAALDALRDLPSGGSVSVIAAGSTARVIANGTTDLGRIRQAIGNLV
                     VEPAAGDLADALRLAGGLASRVADAEIVVATDAAIARPPDVRVDAPVKVLQVGRGRRN
                     QAIVALAVRTAPSSVTRSVFVSVANVDLESADRRLEIWGDGRLLEARDVKLDPLSRSD
                     VGIDDLPRDVSVVEVRLVGAGTGAATAGGDGTAAPDDLAVDDRAWAIVPPDRRRRVLL
                     VGAGDPYLSTALSYLPDTEVYGVAPEAYGPATHPELFDLIVFEGNLPAELPRTAILAV
                     DPPSSSPLGEVVGALKEPAIGTLDPDEPVLHFVDLSTTHIAAARKLVLPAWARTIVPG
                     PGGAPLLYAGQRAGLRTAVLSFAPRQSDLPLQVAFPLLISNLVGELMGGSGAPQTAIA
                     PATPVELAIPAGAIGLRVTAPDGSTTDLVPATPGAATITFSGTGRLGVYEVRPTGDRA
                     SPSPSADGSPAGTAMPLSPPAASSPGTSAAASVTAPPVDPNAPVRFAVDLFSVEESAI
                     APGSAAAIVATGSSARPSTDPGASASPAASASPGASVGPATGAGPIAPRAPARDELWG
                     PLLLAVLGLLLVEWAVYERDGLVRIRRAIGARLPRRRAA"
     gene            complement(5232..6209)
                     /locus_tag="VHM48_15070"
     CDS             complement(5232..6209)
                     /locus_tag="VHM48_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2756790.1"
                     /translation="MAPLGAAPPPDGSSAAAVVGPRSARPAMLENALDPTVFDETFLR
                     QLERLKLLLRQAVRGGLKGGRRSVKRGRSVEFADYREYTLGDDLRQLDWNVYARLERL
                     FVKLFVEEEDVTISILVDASASMASGRPEKLLFAKRAAAALGTIGLASEDRVVVSALS
                     GRAGRRHVALRGSGRVLRLLAELSAIGPADGPTDLLAAVRHAGAQLRGRGLVILISDL
                     LDPSADRVIRDLAATGSELVVIHVLSPEEIDPLLEGDLRLVDSESGAGVDVTVDLSTL
                     DDYRTRLATWRDGFAELAVKRRASYVPVGSDLPIADLVFAELRRRQVAG"
     gene            complement(6236..>6490)
                     /locus_tag="VHM48_15075"
     CDS             complement(6236..>6490)
                     /locus_tag="VHM48_15075"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756791.1"
                     /translation="APQLVRDYVRYGGSPRGVQALVAAGKIAALLDGRFNVAIEDIRA
                     AALPALRHRLILNFEGEAEGITSEAVIRTILESLDPPSVE"
BASE COUNT          890 a         2320 c         2456 g          824 t
ORIGIN      
        1 gtcttgacaa gcgccgtggc ctcgccgcag cgcccaaagg gggactcgcg agccttcgct
       61 cgctgctcgc gggggcggct gatcccgccc tcgcggcgcg cccctcagcc gccccgggcg
      121 cgtcgcttcg cggcgcgcag ccgctccagc gtgtcggcgt cgaggtccag ggccgccgcc
      181 gaaggcgtcg cggcgacggg ggccgactcc gccgcgggtg ccggtgcagc cgcgagcgcc
      241 gccgatgccg ccgatcccct ggccggcctc ggttcccggg agggcgtcgc cgcggccgcc
      301 tccgcctcgg gggtcgtcga ggaatcgcgc cggatcgccg cccgggcggc cgccgagccg
      361 gcccgatccc gggccgccag catcccggtc aagctcgcgg gccgggcggc gacccgccga
      421 tgccgccagc cggcggccca ggcccaggcg gctgcgaact cgcgccgacc gagcgacacg
      481 cgccggaggg cgacatccag cggccagagc agcatcgcga tcacgagcag gagcggccac
      541 aggtcggcgt agtggctcgt ccgcgagagg tcgtgccgcc agacgtcgct cggggcggcc
      601 accacggtcc cgcccgtcgc ggagcggagc gcccccagga gcgtctcgtt cgtgccgagt
      661 cgccggtact cggtggcggt cggggccacg agccccacgg tccgcccgag cggcgcggtc
      721 ccgggtcgcg tctggctgag ccggatcgcg tacgcacccg ggtcgagcgc cccgagcggc
      781 gcctcgtaga tcccgggcgc gacctggacg agcgtggcgt cgaccggctg gagatccggc
      841 ccgacgatct tcgccgtcgt ggcgtagaag tcgcgcggcg agccgtccgc ctcgacgctc
      901 tcgacgtgca gggtcgtctg ttcgcccctg gtgtcgaacc gggcttcgat cccgccggtc
      961 tcctcacccg ggaaggtcca ggccacgagc tggctggcga accggctgaa gtcgcgccag
     1021 ggcagccagt ccttggccca gcgaccggtg gtgtcggagg tccaggccac cgacctcccg
     1081 agtccgtact gccactgggc aaggagcggg tcgtcccggc cggtgaccag gatcgtctgg
     1141 gccgccgcct tggcggtcgt gccgttgtag ccgaggagcg gcggatagcc agcgtcgagg
     1201 ccgcgcagga tcggcgacga ggcggtctgg gccggatgga acggctcctc gacgatctgc
     1261 tggccggaga cctgctgggt ctccttgagg aagatgtcgg ggatcgacga ggggttgacc
     1321 gccgggtagt agcgcccccc gccctgggcg gcgagctggg ccagcaccgg cgccgcacca
     1381 ccgcccgccc cgaccgtcga gagggtgatc ccggccgcct tcatccgggc caggatctgg
     1441 tcgtaggctc cggagcggga ccacccgtcg gtcagcagga tgatgtggcg ccgggtcgcg
     1501 gtcgccgtct cgagcgaacc gaccgccgcg tcgagcccgg cgaagatgtt cgtctggccg
     1561 ttcggctgga tcccggcgat ctggtcctgg aggctggtca cgtccccgag cggctgcgtc
     1621 cggacgatcc agtgggcggc ctcgttgaac ccgacgaccc cgatgctgtc ggtcgcctgg
     1681 agggcggcca cggcccgcag gatcgcctcc ttgccgatgt cgaccttgcg gacgccctgg
     1741 atcgcggtgc caccgctctg gttgaaggtg ttgcagtggc aagcatccat cgagcccgac
     1801 tgatcgatca ccacgaccag ggcgatgtcg ggctgcttcg tcttgtcgcg cacgcccatg
     1861 tcgaccggga gcacctcctc gagcggcgtg ttcgtgtaac cgcccgcgcc gtagctgtgc
     1921 ggcccgccga tcatcacgag cccccggcca aggtcccgga cgtacgactg gagggcggcc
     1981 atcctgcggt cgccgagatc ggtcctgggc acgtcgacga ggacgacgct gtcgtagttc
     2041 gcgagaccgg cgaggtcgct cgggaccgca tccggggcga ccagttcggt ggtctggcgc
     2101 tccgtcgtga gcgcggccac gagctcggcg gcgaccttcg cgtcgccggc caccacgagg
     2161 gtccgcggcg gtcccttgac gatcgtgtcc gagtcggccc ggttgttctt gtcgaacgtg
     2221 tcgcgggccg cctcgaccac cgcccggaag acgtggaagc cgggctccgt cggcttcacc
     2281 cggaaggtca cggccgtcgt gccggccggc aggtcgaccc gctgggtctc tacgagcccg
     2341 ccgtcggcga acagtcggac ggtcgccggc tgcgcgacgg tcgaggtgat cgtcgcggtc
     2401 gcctcgatct cctcgcccag ccggctggtg gtcggcgtcg tcagccgctc gaccaggacc
     2461 tcgtccgtcg ccgtcccgcc gaggagccgg gtctcgatct ggatcccgcg ggaggccgcg
     2521 agggccgcgg cgtcctggcc ggtcccggtc gtgtcgttcc cgtccgagag gaggacgatc
     2581 cggcgctggg cgtcgtccgg gaatagcgcc gaggcgagcc gcagcgccgc gccgatgtcg
     2641 gtcgccgagc ggaccggcgt cgaggcgagc cggtcgatgt cgcggacctc ggctggcagc
     2701 cgctccacca gggcgtcccg cccgaacgcg accaccccgg cgaggtcgtc cctgggcatc
     2761 gccgccagtg cctgccggag gaaggcgagc ccgtcctcgc gcccggcgtt gccgacagaa
     2821 tcggacaggt cgaccacgaa gacggtcgcc agccggtcca tcggcaggat cagctggaag
     2881 ccggcgagcg cgaacgtcag cacgcccagg aggatcgccc gcacgaggag cgccgcccgc
     2941 cgacgcccga taccgagtcg gtggcgtgcc gcggcgtgca cgatcagcgt cagcccgacg
     3001 aggatcggga ggagcagcag gacctccggc cggtcgaacg cgatacccat caggcggccc
     3061 gccgccgggg gagccgagcg ccgatggccc gccggatccg gacgagcccg tcgcgctcgt
     3121 agacggccca ctcgaccagc aggagcccga ggacggccag gagcaacggt ccccacagct
     3181 cgtcgcgggc aggcgcgcga ggagcgatcg gaccggcccc ggtcgcaggg ccgaccgatg
     3241 cgcccgggct ggccgatgcc gcagggctcg ctgacgcgcc cgggtcggtc gacggtcgcg
     3301 cgctcgaacc ggtcgctacg atcgccgccg cgctgccggg tgcgatcgcc gattcctcga
     3361 cactgaacag gtcgaccgcg aacctgactg gggcattcgg gtcgaccggt ggtgcggtca
     3421 ccgacgcggc cgcggacgtg cccggtgacg acgccgcggg cggcgagagc ggcattgcgg
     3481 tgccggccgg cgacccgtcg gcggacggag acggcgaggc tcggtcaccg gtgggccgga
     3541 cctcgtacac gccgaggcgg ccggtgccgc tgaaggtgat cgtcgcggct cccggggtgg
     3601 ccggaacgag atcggtggtc gagccatccg gggccgtcac ccgcaggccg atcgcgcccg
     3661 ccgggatcgc gagctcgacc ggcgtggcgg gcgcgatcgc ggtctgggga gccccactcc
     3721 cgcccatcag ctcgccgacc aggttcgaga tgaggagcgg gaaggccacc tggagcggca
     3781 ggtcggactg ccggggggcg aaggaaagca cggccgtccg caggccggcc cgctggccgg
     3841 cgtacagcaa gggtgccccg ccgggtcccg ggacgatcgt ccgtgcccag gccgggagga
     3901 cgagcttgcg cgctgcggcg atgtgggtcg tcgagaggtc cacgaagtgg aggacgggct
     3961 cgtcggggtc gagcgtcccg atcgcgggct ccttgagcgc cccgacaacc tcgccgagcg
     4021 gcgagctcga gggcgggtcg accgcgagga tggccgttcg cgggagctcg gccgggaggt
     4081 tcccctcgaa cacgatcagg tcgaacagct cgggatgggt cgccggtccg taggcctccg
     4141 gggccactcc gtagacctcc gtgtcgggca ggtacgagag ggccgtcgag aggtacgggt
     4201 cgccggcgcc gacgagaagg actcggcggc gacggtccgg cgggacgatt gcccaggccc
     4261 gatcgtcgac agccaggtcg tctggcgcag ccgtgccatc gccgccggcc gtcgccgcac
     4321 cggtgccggc gccgacgagc cggacctcga cgacactcac atcacgcggc aggtcgtcga
     4381 tcccgacgtc gctccgggat agcggatcga gcttcacgtc gcgtgcctcg aggaggcgac
     4441 cgtcacccca gatctcgagc cggcggtcgg ccgattcgag atcgacgttc gcgacgctca
     4501 cgaagaccga gcgggtgacc gacgagggag ccgtccgcac cgccagggcg acgatcgcct
     4561 ggttccggcg accccgtccg acctggagga cctttaccgg ggcgtcgacc cggacgtccg
     4621 gcggccgggc gatcgcggca tcggtcgcca ccacgatctc ggcgtccgcc actcgcgagg
     4681 cgaggccacc cgcaaggcgg agtgcatcgg cgaggtcgcc cgccgccggc tcgacgacga
     4741 ggttgccgat ggcctgtcgg atccggccga ggtcggtcgt cccgttcgcg atgacccggg
     4801 cggtggagcc ggccgcgatc acgctcaccg agcccccgct cgggaggtcc cggagcgcgt
     4861 cgagcgcggc ttgcttcgcg gcctcgagac ggttcgggaa gacgtcggtc gcccccatgc
     4921 tcgcgctcgt gtcgatgacc aggacgatgt cgcgggcgag gccagccggt cgttcgacga
     4981 acgggcgcgc ggccaggacc gccaggaccg cgaccagcag gagctgaagg agcagcagga
     5041 ggctgcgccg cagccgttgc cagggcgcgt tcgcctcgac gtcggccacg agccgctgcc
     5101 agaggagcgt cgacgggacg acgtgctcgt cgcggcggag cttcaggagg tagaacgcga
     5161 cgacgagcgg gatgaaggcc aggcccgcca gcgccagcgg cgcgatcagc ggcacggtgg
     5221 cctcgcggcg ctcaacccgc cacctggcga cgccgcagct cggcgaagac caggtcggcg
     5281 atcggcaagt cggacccgac aggcacgtag ctcgcccggc gcttcacggc gagctcggcg
     5341 aatccgtcgc gccaggtggc cagccgggtc cggtagtcgt cgagcgtcga gaggtcgacc
     5401 gtcacgtcga cgcccgcgcc gctctcggaa tcgacgagcc ggaggtcacc ttcgaggagg
     5461 gggtcgatct cctcagggct caggacgtgg atgacgacga gctcggaacc cgtcgcggcc
     5521 aggtcccgga tcacccgatc ggccgacggg tcgaggagat cggagatgag gatgacgaga
     5581 ccccgaccgc ggagctgggc gccggcgtgg cggaccgccg cgagcaggtc cgtcggtccg
     5641 tcggccggtc cgatcgccga gagctcggcg agcagccgga ggacccgccc cgaaccgcgg
     5701 agcgcgacat gccgccggcc ggctcggcca ctcagcgcgc tgacgaccac ccggtcctcc
     5761 gatgccagcc cgatcgtccc gagggcggcg gccgcccgct tggcgaacag gagcttctcc
     5821 ggtcggccgc tcgccatcga cgcgctggca tcgacgagga tgctgatggt gacgtcctcc
     5881 tcctcgacga acagcttcac gaacagccgc tcgaggcggg cgtagacgtt ccagtcgagc
     5941 tggcgaagat cgtcgcccag ggtgtattcg cggtagtcgg cgaattcgac cgagcggccc
     6001 cgcttgacgc tccgtcggcc gcccttgagc ccgccccgaa cggcctgccg gaggaggagc
     6061 ttcaggcgct cgagctggcg gagaaacgtc tcgtcgaaga cggtcgggtc gagggcgttc
     6121 tcgagcatgg ccggacgcgc gctgcggggg ccgacgaccg cggctgccga cgagccgtcg
     6181 ggcggcgggg cggcaccgag cggagccatc gcagtccgac cctcgggtcc cggagctact
     6241 cgacgctcgg cgggtcgagg ctctcgagga tcgtccggat caccgcctcg ctcgtgatgc
     6301 cctccgcctc gccctcgaag ttgaggatga gtcggtgccg cagcgcgggc agggccgctg
     6361 cccggatgtc ctcgatggcc acgttgaacc ggccgtcgag cagggccgcg atcttgcccg
     6421 cggcgaccag cgcctggacg ccccgcggcg agccgccgta gcgaacgtag tcgcggacga
     6481 gctggggtgc
//
LOCUS       DATAIW010000514         7618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_261185, whole genome shotgun sequence.
ACCESSION   DATAIW010000514 DATAIW010000000
VERSION     DATAIW010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261185"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VHM48_15080"
     CDS             <1..337
                     /locus_tag="VHM48_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX2756792.1"
                     /translation="INGYTYRSTVAPMGGRYLIGVSAENRAAAGVRGGESHDVELTLD
                     TVPREVELPADFAAALAADPEARATFDALSPSNKGYHTVLIEGAKSDETRLRRLAKSV
                     AALHEGRPR"
     gene            complement(367..2172)
                     /locus_tag="VHM48_15085"
     CDS             complement(367..2172)
                     /locus_tag="VHM48_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEX2756793.1"
                     /translation="MFSAPAASPAPRSSQRAGARPASVVALAVLLLLGSVSGTSAAAR
                     PAGSGGDGTDRSGVTSDATGTVLEGLDVSHWQGTINWTSVAGAGKKFAIIKATDGQVQ
                     SNGTLYVDPMYATNHSRAKAAGLWTGAYHFARPGSNTNDAIIEADHFAANINIGAGDL
                     IPALDLEDSGGLSVAGLQAWVASFLGEVTGKIGARPMIYTSPAFWKKYMGDSHALADA
                     GYTTLWVAHWGVTSPTVPASNWGGHGWTFWQYTSDGTVAGISGRVDLDRFNGTDLATQ
                     AYSTFKLAASIPSGSVKQGSSAAATVSIIRLNFASDIALAVSGLPEGTTVTYDANPVD
                     DASAALTVTTPADPAATPVGTYPLTITGAADGITRTTKLNLVIADGIPPTLTAPTTHL
                     WANRTLGTSTVPVRIAWTATDPSGIVSTGLQRSLNGGSWTATSLPSATATAADGSIPI
                     GGTARQRVRASDTRANTSGWLEGNLVRASVYQQSSTALTWTGTWHTSGWSGASGGTVR
                     YATARGASATFHFTGSSVAWVAARGTTRGSAWVYVDGVYAGSVSLHATAGHSRAIVFA
                     RNWSGIGSHTLRIVVAGTAGHARVDVDAFVRLTAG"
     gene            2431..2919
                     /locus_tag="VHM48_15090"
     CDS             2431..2919
                     /locus_tag="VHM48_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX2756794.1"
                     /translation="MGVFVVSPSRRLGQSLIEFALILPILLTLLGAAVDIARLYQGWI
                     TLEGATRDAAEYAATFDKTSTAATTDAKRIICTQTATLPGFAAPPGNPTACTSPAITI
                     TSFPAGDTAAANGGSAVNPVQSITVTATLPFRTFFGYPLFTQGGAWTLTSTQSYSIIQ
                     GR"
     gene            2921..3424
                     /locus_tag="VHM48_15095"
     CDS             2921..3424
                     /locus_tag="VHM48_15095"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX2756795.1"
                     /translation="MRRRRSTGQALLEFSLVLPIFLVMLMGVVDVGRAIWAQNSLAAA
                     AREGARFAIVHGGSMTTACPVGPPVADVTIIPPASAPCPYPSPSTQAIKDAVIAAANA
                     GGTSLTVTVCYGTGCSGDTNALNATNARGTPVTVAVSSRINLITPSLLGMGGFGVSSV
                     TTMVVNH"
     gene            3424..4866
                     /locus_tag="VHM48_15100"
     CDS             3424..4866
                     /locus_tag="VHM48_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEX2756796.1"
                     /translation="MLRFARRRDRRASGQIIVLFVLSLTAIFAMAALLFDGAHALVLR
                     RQIQNAGDSAALQAANLIRNLKGCSATYVMGSAPGSPRAAITTAAQTAVQAALPGTPA
                     SAIHVSCPDGIWNNYAVQVDVDGHSPTFFGGAVGIRGFDVKTTSQAVNGPVAPAPYSV
                     ILLDNSNSSWPNGRRGCPAALISGGPTVIFEGSMMIDSACTAANGGGLGTNGNSATLT
                     FNNSAVIKIVGGYAPAALTITPTPTTGAAYVKDPLLGLPAMPSTLPVRFASRQVYGGS
                     DVVLEPGVYVGGIQLKNSVKAFLHPGVYEFRGGGFDIGAQNAVYSVATGVTTTTEANW
                     ATNCPVASCGVLFYNSGTTSTMGQVTVGAGATLKLRPYNSTYDGAGANQPDYNNLLLW
                     QSGTPAPSNSFEQPVVSLSGGGSVDISGTVYAPGAVVFMTGGSGGSGGGTDLTLQFIC
                     WDLQIQGNSSFHFFYQNNSFAHPPDYGLIK"
     gene            5090..6445
                     /gene="mgtE"
                     /locus_tag="VHM48_15105"
     CDS             5090..6445
                     /gene="mgtE"
                     /locus_tag="VHM48_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX2756797.1"
                     /translation="MSTVATDALLEEIQELIREREQDGVAGLAERVGPAEWAALVPRL
                     DPSEVAVLLQWLPDEEIPQLLEELDPHEAAAILRTLSRQEAARLLAGMDPDDAADVVE
                     NLTEPEVSQILVRMDPAEAEEIRRLSDFAPDSAGGIMTPEFVAVTTDATADSAIAAIR
                     GLVGQAETVDYVYVVDAERHLIGVLSLYRLMLSPGHTPVTALIAPVTVRVRADADRET
                     AANLLTERNLLAIPVVDDADHLLGIITGDDVADVLEAEATEDIERLGGSQPLNVPYRL
                     SSVGLLVRKRIGWLLLLFIAEAYTGSVLRAFSTELEAAVALSFFIPLLIGTGGNVGSQ
                     TVTLIVRAMALNEISLRDVGWIVWKELRVGLIMGLVMAAVALGRAQLLGVGTNIGLVV
                     AVTILVICLWSATVAAVLPLTLRRLRIDPAVVSAPLITTLVDGTGLIIYFEIAKVLLR
                     I"
     gene            6570..>7618
                     /locus_tag="VHM48_15110"
     CDS             6570..>7618
                     /locus_tag="VHM48_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2756798.1"
                     /translation="MDAADRTAELLDSAVRFEASARLIFEIAAAASGELDLDQILHEA
                     LDRLRSVVRLTGGSIALVDGDELIVRAAIGPFSDEALGQRLRRGPSRSWGVVERLEPY
                     LTGDLFRTGDRVKGAAAATAVRSWLAVPIARHGVGIGLIEIDSTEIDAFTPADQELLA
                     GVVAVLGGHVELAAHHADEQRSAALREAFVGVISHELRTPITTIYGLARVLRQRGDTL
                     DATARNEAIADIEAESDRLYRLVEDLLVLSRAESGRVEIEPEPINLTRLVGHIVEAEG
                     QRQPDRSFLLTAPAGLPLIEAEATYVEQVVRNFLTNAVKYSEPPTVVEVVVGVDGDDV
                     VVQVLDRGIGVDEEAA"
BASE COUNT         1096 a         2794 c         2586 g         1142 t
ORIGIN      
        1 gatcaatggc tacacctacc ggagcacggt cgcgccgatg ggcggccgct atctcatcgg
       61 cgtcagcgcc gagaaccggg ccgccgccgg cgtgaggggc ggcgagtcgc acgatgtcga
      121 gctgacgctg gacacggtcc cacgcgaggt cgagctaccg gctgacttcg ccgccgccct
      181 ggccgcggat cctgaggctc gggcgacgtt cgacgcgctc tcgccgagca acaagggcta
      241 tcacacggtg ttgatcgagg gcgcgaagag cgacgaaacc cgcctgcggc ggctcgcgaa
      301 gtcggtcgcg gcgctccacg agggccgccc tcgctgaacc ggtgcccggg cccgcccgga
      361 catcggtcag ccggccgtca gccggacgaa ggcgtcgacg tcgacccggg cgtggcccgc
      421 cgtcccggcc accacgatcc tgagcgtgtg actcccgatc ccggaccagt tccgggcgaa
      481 cacgatggcc cgcgaatggc cggcggtcgc gtggagcgag accgatccgg cataaacacc
      541 gtcgacatag acccacgccg atccgcgggt cgtgcccctg gcggcgaccc aggcgacgct
      601 cgatccggtg aaatggaagg tcgccgaggc cccgcgtgcc gtcgcatagc gcacggttcc
      661 gcccgacgcg ccggaccagc cgctcgtgtg ccacgtgccg gtccaggtca gcgcggtcga
      721 cgactgctga tacacgcttg ccctgacgag attgccctcc agccacccgc tcgtgttggc
      781 ccgcgtgtcc gaggcccgga cgcgctggcg ggccgttccg ccgatcggga ttgaaccgtc
      841 ggcggccgtg gcggtcgcgc tcgggagcga tgtcgccgtc cacgaaccgc cgttgaggct
      901 ccgctgcagg ccggtcgaca cgatcccgct cggatcggtc gcggtccagg cgatccggac
      961 cggcaccgtc gaggtcccga gggtccgatt ggcccagagg tgggtggtcg gcgccgtgag
     1021 cgtcggggga atcccgtcgg cgatgacgag attcagcttc gtcgtccggg tgatcccgtc
     1081 ggccgcaccg gtgatcgtca gcggatacgt tccgacaggg gtggcggccg ggtcggcggg
     1141 ggttgtgacc gtgagcgctg ccgacgcgtc gtcgaccggg ttggcgtcgt acgtgacggt
     1201 ggtgccttcc ggcaggccgc tgaccgcgag cgcgatgtcg gaggcgaagt tcagccggat
     1261 gatcgacacg gtcgcggcgg cgctcgagcc ttgtttgacc gacccgctcg ggatggacgc
     1321 ggcgagcttg aacgtcgagt aggcctgggt tgcgaggtcg gtaccgttga accggtcgag
     1381 gtcgacccgg ccggagatcc ccgccaccgt gccgtcgctc gtgtactgcc agaacgtcca
     1441 gccgtggccg ccccagttgg aggccgggac cgtggggctc gtgactcccc agtgcgcgac
     1501 ccacagggtc gtatagccgg cgtcggcgag ggcatggctg tcgcccatgt acttcttcca
     1561 gaaggccggg ctcgtgtaga tcatcggccg ggcgccgatc ttcccggtga cctcgccgag
     1621 gaaggacgcc acccacgcct ggagccccgc cacgctgagg ccgccgctgt cctcgagatc
     1681 gagcgccggg atcagatcgc cggccccgat gttgatgttg gcggcgaagt ggtcggcctc
     1741 aatgatcgcg tcgttggtgt tgctgcccgg ccgggcgaag tggtacgcgc ctgtccacaa
     1801 cccggctgcc ttcgcccggg aatggttcgt cgcgtacatc gggtcgacat aaagcgtgcc
     1861 attgctctgg acctggccgt cggtcgcctt gatgatcgcg aacttctttc ccgcgccggc
     1921 gacggaggtc cagttgatcg tgccctgcca gtggctgaca tcgagcccct cgaggaccgt
     1981 tccggtcgcg tcggaggtca ccccggatcg gtcggtcccg tctccgccgc tccccgctgg
     2041 ccgcgccgcg gccgacgttc cggacaccga gccgaggagg agcaggaccg cgagcgcaac
     2101 gacggacgcg gggcgcgcgc cggcccgctg cgatgagcgc ggggcgggcg aggcggcggg
     2161 cgcagagaac aaggtcgtga cggctccagc ggcgggttcg caaggacccc tcgaccatcg
     2221 aacgccgccc ctgcctgttc aatagcctaa acgggagggc aagtcggtcc gggcccggta
     2281 cgttcggccc gcgtccgcct ggtcccggtc cgccggtccg gccgcaccga cgaaacgcgg
     2341 gctcgggact tccggcacgg cgctccccgg acttccgggt ggcggctcac gggcacccct
     2401 cgatgggagg ctgaccaggt cacccgagcc atgggagttt tcgtggtcag cccgtcacgc
     2461 cgtcttggcc agtccctcat cgaattcgcg ctgatcctgc cgatcctgct gaccctgctg
     2521 ggtgccgcgg tcgacatcgc gcgcctgtac cagggctgga tcacgctcga gggcgcgacc
     2581 cgcgatgccg ccgagtacgc ggcgacattc gacaagacct cgacagctgc aaccaccgac
     2641 gcgaaacgga tcatctgcac ccagaccgcg accctgcccg ggtttgcggc gccgcccggc
     2701 aacccgaccg cgtgcacctc gccggcgatc acgatcacct cgttcccggc cggcgacacc
     2761 gccgccgcga acggaggctc ggcggtcaac ccggtgcagt cgatcacggt gaccgcgacg
     2821 ttgcccttcc ggacgttctt cggctacccg ctgttcaccc agggcggggc ctggacgctg
     2881 acctcgaccc agtcctactc gatcatccag ggacgctgac gtgcgacgcc gtcgatccac
     2941 cggccaagcc ctcctcgagt tctccctggt cctgccgatc ttcctcgtca tgctcatggg
     3001 cgtcgtcgac gtcggccggg cgatctgggc ccagaacagc ctcgccgccg cggcccgcga
     3061 gggcgcgcgc ttcgcgatcg tccacggcgg ctccatgacg acggcctgtc cggtcggacc
     3121 gccggtcgcg gacgtgacga tcatcccgcc ggcatctgcc ccttgtccct atccgtctcc
     3181 ctcgacccag gcgatcaagg acgcggtcat cgccgcggcc aacgccggcg ggacgagcct
     3241 gaccgtgacc gtctgctacg gcacgggctg cagcggcgac acgaacgcgc tcaacgcgac
     3301 gaacgcccgg gggacgccgg tcacggtggc cgtcagcagc cggatcaacc tgatcacgcc
     3361 gtcgctcctc ggcatgggcg gcttcggggt ctcctcggtc acgaccatgg tcgtgaacca
     3421 ctgatgctcc gcttcgcacg ccgccgcgac cgccgcgcgt ccggccagat catcgtcctg
     3481 ttcgtcctgt cgctgacggc gatcttcgcg atggcggcgc tcctcttcga cggcgcccac
     3541 gcccttgtcc ttcggcgcca gatccagaac gccggcgaca gcgccgccct ccaggccgcc
     3601 aacctgatcc ggaacctcaa gggctgttcc gcgacctacg tcatgggctc ggcccccggc
     3661 agcccgcggg ccgcgatcac gacggccgcc cagaccgcgg tccaggccgc gctgcccgga
     3721 acaccggcga gcgcgatcca cgtctcctgc ccagacggga tctggaacaa ctacgccgtg
     3781 caggtcgacg tcgacggtca ctcgccgacg ttcttcggcg gcgcggtcgg catccgcggc
     3841 ttcgacgtca agacgacgag ccaggcggtg aacggcccgg tcgcgccggc cccgtactcc
     3901 gtgatcctgc tcgacaactc gaactccagc tggccgaacg gccggcgcgg ttgcccggcg
     3961 gccctgatct cgggcggacc gacggtcatc ttcgaggggt cgatgatgat cgactccgcc
     4021 tgcaccgcgg cgaacggcgg cgggctcggc acgaatggca actcggccac tctgacgttc
     4081 aacaacagcg ccgtcatcaa gatcgtcggc ggctacgccc cagcggctct gacgatcact
     4141 ccaacgccga cgaccggcgc cgcctacgtc aaggatccgc tcctgggcct cccggcgatg
     4201 ccgtccaccc tgccggtccg gttcgcgtca cgccaggtct acggcggctc ggacgtggtc
     4261 ctcgagcccg gggtctacgt cggcgggatc cagctgaaga actcggtcaa ggcgttcctc
     4321 catccggggg tctacgagtt ccgcggcggg ggcttcgaca tcggcgccca gaacgcggtc
     4381 tactcggtcg caaccggcgt cacgacgacg acggaggcga actgggcaac caactgcccg
     4441 gtcgcgagtt gcggggtcct cttctacaac agcggcacga cgtccacgat gggacaggtg
     4501 accgtcggcg ccggcgcgac gctgaagctc cggccgtaca actcgacgta cgacggcgcg
     4561 ggcgccaacc agccggacta caacaacctg ctcctctggc agagcggcac cccggctccg
     4621 tccaacagct tcgaacagcc ggtcgtctcg ctgagtggcg gcgggtcggt cgacatcagc
     4681 gggacggtct acgcgccggg ggccgtggtc ttcatgaccg gcggctcggg cggctcgggc
     4741 ggcggcacgg acctgaccct ccagttcatc tgctgggacc tccagatcca gggcaactcg
     4801 agcttccact tcttctacca gaacaacagc ttcgcccacc cgccggacta cggcctgatc
     4861 aagtagcccg tcggcccgcc gcggggtcgg accggcccgt tcgcaggcct cgcggccatg
     4921 tccggtcccg gccggcttgc cgccacgtcc ggcaccggcc ggcgcgcccg ccagcgatcg
     4981 agtctgacgg tacgatgcga ccgcacgccg gcctgtcccg ccggcccgag gtcaaccatc
     5041 tccgcggccg gctccccggc cgccccaccc gtcctgtccc gaggctgcca tgtcgaccgt
     5101 ggccaccgac gccctgctcg aagagatcca ggagctgatc cgcgaacgcg agcaggacgg
     5161 cgtcgccggg ctcgcggaac gcgtcggacc cgcggaatgg gcggccctcg tcccgcgcct
     5221 cgacccgagc gaggtcgccg tcctcctcca gtggctcccc gacgaggaga tcccccagct
     5281 cctcgaggag ctcgatccgc acgaggcggc cgcgatcctc cggacactga gtcgccagga
     5341 agccgcccgg ctccttgccg gcatggatcc cgacgatgcc gccgacgtcg tcgagaacct
     5401 gaccgagccc gaggtcagcc agatcctcgt ccggatggac ccggccgagg ccgaggagat
     5461 ccgccgcctg tccgatttcg cacccgacag cgccggcggg atcatgaccc cggagttcgt
     5521 cgcggtcacg accgacgcga ccgccgacag cgcgatcgcc gccatccgcg gtctcgtcgg
     5581 ccaggcggag acggtcgact atgtctacgt cgttgacgcc gagcgacacc tgatcggcgt
     5641 cctgtcgctc tatcgcctga tgctcagccc gggccatacg ccggtgacgg cgctcatcgc
     5701 tccggtcacg gtccgggtcc gggccgacgc cgaccgggag acggccgcca accttctgac
     5761 cgagcgcaac ctcctcgcga tcccggtggt cgacgacgcc gaccacctcc tcgggatcat
     5821 caccggtgac gacgtggccg acgtcctcga ggccgaggcg accgaggaca tcgagcgcct
     5881 cggcggctcg cagccgctga acgtgccgta ccgcctgtcg agcgtcggcc tgctcgtgcg
     5941 caagcggatc ggctggctgc tcctcctgtt catcgccgag gcctacacgg gcagcgtcct
     6001 gcgggcgttc tcgaccgagc tcgaggcggc cgtcgccctc agcttcttca tcccactgct
     6061 gatcgggacc ggcggcaacg tcggcagcca gacggtcacc ctgatcgtcc gggcgatggc
     6121 cctcaacgag atctccctgc gcgacgtcgg ctggatcgtc tggaaggagc tccgggtcgg
     6181 gttgatcatg ggcctggtca tggccgcggt cgcgctcggc cgggcccagc tcctcggggt
     6241 cgggacgaac atcgggctcg tcgtggccgt cacgatcctc gtcatctgcc tctggtcggc
     6301 gaccgtagcc gccgtcctgc cactgaccct gcgccgcctc cggatcgatc cggcggtcgt
     6361 gtcggcgccg ctcatcacga ccctggtcga cggcaccggg ctgatcatct acttcgagat
     6421 cgccaaggtc ctgctccgga tctgaggggt ccgcgacggg tcgcgttccg tgccggcgca
     6481 agcggctcga tattgtggcc ttctggccgg cgccgcaccc gggctgatcg gcccgggcga
     6541 aacgggtcgc ggcgggtagc atgaggccag tggacgccgc cgaccggacc gccgagctgc
     6601 tcgactccgc cgttcgattc gaggcttcgg cgcgcctcat cttcgagatc gcggcggccg
     6661 cgtccggtga gctggacctc gaccagatcc tccatgaagc actcgatcgg ctccgttcgg
     6721 tcgtccggct caccggaggg tcgatcgccc tcgtcgacgg cgacgaactg atcgtccggg
     6781 ctgccatcgg tccgttctcg gacgaggcgc tcgggcagcg cctccggcgc gggccgagcc
     6841 ggagctgggg cgtggtcgaa cggctggagc cgtacctgac cggagacctg ttccggaccg
     6901 gcgatcgagt gaagggtgcc gccgccgcga ccgcggtccg ctcatggctg gccgtcccga
     6961 tcgcgaggca tggcgtcggg atcgggctga tcgagatcga ctcgaccgag atcgacgcct
     7021 tcacgccggc cgatcaggag ctgctcgccg gcgtcgtggc cgtgctcggg ggccacgtcg
     7081 agctggccgc ccatcacgcc gacgagcagc ggtcggcggc cctccgggaa gcgttcgtcg
     7141 gggtgatcag ccacgagctg cggacgccga tcacgacgat ctacggcctc gcccgcgtcc
     7201 tccgccagcg tggcgacacg ctcgatgcaa ctgcgcggaa cgaagccatc gccgacatcg
     7261 aggcggagtc cgatcggctc taccgactcg tcgaggacct cctcgtcctg tcgcgagcgg
     7321 agagcgggcg ggtcgagatc gagcccgagc ccatcaacct cacccgcctg gtcgggcaca
     7381 tcgtcgaagc ggagggccag cgccaaccgg accggagctt cctcctcacc gcccccgccg
     7441 gccttcccct gatcgaggcc gaggcgacct acgtcgagca ggtcgtccgg aacttcctga
     7501 cgaacgccgt caagtacagc gagccgccga cggtcgtcga ggtcgtcgtc ggcgtcgacg
     7561 gcgacgacgt ggtcgtccag gtcctcgatc gcggcatcgg cgtcgacgag gaggccgc
//
LOCUS       DATAIW010000515         5318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_143435, whole genome shotgun sequence.
ACCESSION   DATAIW010000515 DATAIW010000000
VERSION     DATAIW010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143435"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1513)
                     /gene="xylB"
                     /locus_tag="VHM48_15115"
     CDS             complement(26..1513)
                     /gene="xylB"
                     /locus_tag="VHM48_15115"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX2756799.1"
                     /translation="MTFVLGIDVSTTATKAVLVDATGSVAGIGVAEYPYDIPQPLWSE
                     QDPGLWWTGAVEAIRSVLASTGVAGDDVAAIGLTGQMHGAVLLDASDAILRPAILWND
                     QRTAAECDAIRAAVGPERLIAITGNDALTGFTAPKLVWVRDHEPDIWRRVAHVLLPKD
                     FVRLRLTGDHALDKADGAGTLLFDLAARDWSPKVLDALGIDPAWMPPTFEGPQPTGTV
                     TATAAAATGLRAGTPVVAGGGDQAANAVGVGVVDPGSVALSLGTSGVIFAATDRPAFD
                     PLGRVHAFCHAVPERWHLMSVMLSAAGSLRWYRDTLAPGVDFGALIAEAGDIPAGSDG
                     LLFLPYLTGERSPYPDPLARGGFIGLTVRHDRRHLTRAVLEGVALGLRDGLDLMTAAG
                     MPAPTQIRASGGGTASALWRQILADVLEAEIATVDTTEGAAYGAALLAAVGAGWYATV
                     GEVTDALTSATPAAAPGPDAAAYREAHDRYRELYPALAPTFHRTH"
     gene            complement(1529..2896)
                     /gene="xylA"
                     /locus_tag="VHM48_15120"
     CDS             complement(1529..2896)
                     /gene="xylA"
                     /locus_tag="VHM48_15120"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX2756800.1"
                     /translation="MADGFFSTVPDRISFGGPDSTDPLAFKVYEPDRIVLGKRMEDHL
                     RIGVCLWHSFAWDGRDMFGVGTLDRPWLDPAAEPMPAARQKMAVAFEFIEKLGTPYYC
                     FHDRDVAPEGASFAEFRDNLDALTDDALGYQERTGATLLWGTANLFTHPRYQGGAATN
                     PDPDVFAYAAAQVKHMLEVTQRLGGTNYVLWGGREGYDTLLNTDLRREGDQLARFLHL
                     VADHKHRIGFEGTLLIEPKPMEPTKHQYDYDSATIHGFLVRNGLEDEYRLNIEANHAT
                     LAGHSFHHEVAYAVANGMLGSIDANRGDPQNGWDTDQFPNSVEDLALPIYEILRGGGL
                     TTGGFNFDAKLRRQSTDRNDLFHAHIGGIDTLARALLVAAALIERETLSKPLEERYAG
                     WNGVLGAAILDGRETLASLEARVASGEIDPSPVSGAQERLEDAVNQVIWTAGRPGPVE
                     PGAER"
     gene            3027..5231
                     /gene="ligA"
                     /locus_tag="VHM48_15125"
     CDS             3027..5231
                     /gene="ligA"
                     /locus_tag="VHM48_15125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2756801.1"
                     /translation="MTDPEMADAAIATVDADTLPVDPALVSEAAALDEPTAAARHAEL
                     AAEIAAANTAYYDADAPTMTDADYDQRFRRLVALEAAWPALVTPTSPTQRVGAQLAGT
                     FDEVRHRRPMLSLSNAFSHDELRAFDTRVRRGLGLLAAPEPAPDLAYVAELKIDGLAI
                     TLRYERGRFVQGATRGDGTTGEDVTANLRTIATVPARLAEPATLDARGEVYMPKAEFA
                     RINAEREEAGFALYANPRNSGAGSLRQKNPQVTASRQLSTWAYQLIEDEDEDARPAAA
                     GVASQSAALDRLAALGFAVNPNREAGLDIEGVIAFTESWRDGRHHLPYETDGVVVKVD
                     RFDQQARLGMVSRAPRWAIAYKFPPEQVETILEDIVPYVGRTGTLTPVAHLTAAKVAG
                     STVTRATLHNLDEIGRKGLLIGDHVILQKAGDVIPEVVRPIVEKRTGAERPFAMPERC
                     PVCATPIVRDEGAVRHYCPNLACPARVSQEFQHFVGRGGMDIEGAGWKVLEQLLQTRL
                     VQRRGDFYRLSVEDLEGLERFGRKSAENLHAAIQKSRRRPLERILAGLGIPQVGWTTA
                     VDLAAWLAGEVPPGDDWLRRAAAHLERLATEEPARFEEVEGIGPTVSTALAAWFAADG
                     PGRGVLEDLADAGVEPELPTPRPAGGRESAVDGPLAGRTVVVSGSIEGYSRDEAEQAV
                     RAAGGKPSGSVSKKTDYLVAGPGAGSKLARAEELAIPVLDAEGFAKLLRGEG"
BASE COUNT          750 a         1895 c         1946 g          727 t
ORIGIN      
        1 cgatcggcgg ggatccgggg cgctctcagt gcgtccggtg gaacgtcggc gccaacgcgg
       61 ggtacagctc gcgatatcgg tcgtgcgcct cgcggtaggc cgcggcgtcc gggccggggg
      121 cggccgccgg cgtcgccgag gtcagcgcat ccgtgacctc gccgacggtg gcgtaccagc
      181 cggcgccgac ggcggccagg agggcggccc cgtaggccgc tccctcggtc gtgtcgaccg
      241 tcgcgatctc cgcctcgagg acatcggcga ggatctggcg ccagagggcg ctggcggtgc
      301 cgccgccgga ggcacggatc tgcgtcggcg ccggcatccc cgccgcggtc atcaggtcga
      361 gcccgtcgcg caggccgagg gcgacgccct cgaggaccgc gcgcgtcagg tggcggcggt
      421 cgtggcggac ggtcagcccg atgaacccgc cgcgggccag cgggtccggg tacgggctcc
      481 gctcgccggt caggtagggc aggaacagga ggccgtcgct gccggcgggg atgtcgcccg
      541 cctccgcgat cagggcgccg aagtccacgc ccggtgcgag cgtgtcgcgg taccagcgca
      601 ggctgccggc ggccgacagc atcaccgaca tcaggtgcca gcgctcgggg acggcgtggc
      661 agaacgcgtg gacccgcccg aggggatcga aggcggggcg gtcggtggcg gcgaagatga
      721 cgccggacgt cccgagcgag agggcgacgg agcccgggtc gacgacgccg acgccgaccg
      781 cgttggcggc ctggtcgccg ccgccggcca cgaccggcgt gcccgcccgc aggcccgtcg
      841 ccgcggccgc cgtcgcggtc accgtgccgg tcggctgcgg gccctcgaac gtcggcggca
      901 tccaggccgg atcgatcccg agcgcgtcga ggaccttcgg cgaccagtcc cgcgccgcga
      961 ggtcgaagag gagggttccg gcgccgtccg ccttgtcgag ggcatggtcg ccggtcagcc
     1021 gcaaccggac gaagtccttg ggcaggagga cgtgggcgac ccggcgccag atgtcgggct
     1081 cgtgatcgcg gacccagaca agcttcgggg cggtgaaccc ggtgagtgcg tcgttgccgg
     1141 tgatcgcgat cagccgctcc ggtccgaccg ccgcccggat cgcgtcgcac tccgcggccg
     1201 ttcgctggtc gttccagagg atcgccggcc ggaggatggc atcggacgcg tcgagcagga
     1261 ccgcgccgtg catctggccg gtgagcccga tcgccgcgac gtcgtcgccg gcgacgcccg
     1321 tggacgcgag gaccgagcgg atcgcctcga ccgcgccggt ccaccacagg cccgggtcct
     1381 gctcgctcca gagcggctgc gggatgtcgt acgggtattc ggcgacgccg attcccgcga
     1441 cggacccggt cgcgtcgacg aggacggcct tcgtcgcggt cgtcgagacg tcgatcccga
     1501 ggacgaacgt cacgtcaggg ccgcccgatc agcgctcggc accaggctcg acgggtccgg
     1561 gccgaccagc ggtccagatg acctggttga ccgcgtcctc gagccgctcc tgagcgccgg
     1621 agaccgggct cgggtcgatc tcgccggagg cgacccgggc ctcgagcgat gcgagcgtct
     1681 cccggccatc gaggatcgcc gcccccagca cgccgttcca gcccgcgtag cgctcctcga
     1741 gcggcttcga gagcgtctcg cgctcgatca gcgcggcggc gacgagcagg gctcgcgcga
     1801 gcgtgtcgat gccgccgata tgggcatgga acaggtcgtt tcgatcggtg ctctggcgcc
     1861 gcagcttcgc gtcgaagttg aacccgccgg tcgtgagccc gccgccgcgc aggatctcgt
     1921 agatcggcag cgccaggtcc tcgaccgagt tcgggaactg gtcggtatcc cagccgttct
     1981 ggggatcgcc ccggttcgcg tcgatcgaac cgagcattcc gttggccacc gcataggcga
     2041 cctcgtgatg gaacgaatgc ccagcgagcg tggcgtggtt ggcctcgatg ttcaggcggt
     2101 actcgtcctc gaggccgttc cggacgagaa agccgtggat cgtggcggag tcgtagtcgt
     2161 actggtgctt cgtcggctcc atcggcttgg gctcgatcag gagcgtcccc tcgaacccga
     2221 tccgatgctt gtggtcggcg acgaggtgga ggaagcgcgc gagctggtcg ccttcgcgcc
     2281 tgagatccgt gttgaggagc gtgtcgtagc cttcgcgccc gccccacagg acgtagttcg
     2341 tgccgcccag ccgctgggtg acctcgagca tgtgcttgac ctgtgcggcc gcgtaggcga
     2401 agacgtccgg gtcgggattt gtcgccgccc cgccctggta gcggggatgg gtgaagagat
     2461 tggccgtgcc ccacaggagc gtcgcgccgg tccgctcctg gtatccgagc gcgtcgtctg
     2521 tgagggcatc gaggttgtcg cggaactcgg cgaacgacgc gccctcgggg gcgacatccc
     2581 gatcgtggaa gcagtagtac ggcgtgccca gcttctcgat gaactcgaac gcgacggcca
     2641 tcttctggcg ggccgccggc atcggctccg ccgccgggtc gagccagggc cggtcgagcg
     2701 tcccgacgcc gaacatgtcg cgaccgtccc aggcgaacga gtgccacagg cagacgccga
     2761 tccggagatg atcctccatg cgcttcccga ggacgatcct gtccggctcg tagaccttga
     2821 acgccagagg gtccgtcgag tccgggccgc cgaacgagat gcggtccggc accgttgaga
     2881 agaacccgtc agccatgtcc aaccctcccg acgccggcga cggagccgac cgccggacct
     2941 ctcacgcgaa tttgtacgag cctaacacaa atgtccatgt gctcggcagg cacccccgca
     3001 ccggccgaat tcgtatcctg tcgagcatga ccgatccgga gatggccgac gccgcgatcg
     3061 cgacggtcga cgccgacacg ctgcccgtcg acccggccct ggtttcggag gccgccgcgc
     3121 tcgacgagcc gacggcggcc gcccgccacg ccgagctggc cgcggagatc gcggccgcga
     3181 acacggccta ctacgacgcc gacgccccga cgatgaccga tgccgactac gaccagcggt
     3241 tccgccggct ggtcgccctc gaggcggcct ggccggccct tgtcacgccg acctccccga
     3301 cgcagcgggt cggcgcccag ctggccggga cgttcgacga ggtccgtcac cggcggccga
     3361 tgctgtcgct ctccaatgcc ttcagtcacg acgagttgcg ggccttcgac acccgcgtcc
     3421 ggcgcggtct cggcctgctc gcggccccgg agccggcccc ggacctggcc tacgtcgccg
     3481 agttgaaaat cgacggcctg gcgatcaccc tccgctacga gcgcggccgg ttcgtgcagg
     3541 gcgcgacccg tggcgatggc acgaccggcg aggacgtcac cgccaacctg cggacgatcg
     3601 cgacggtgcc ggcccgcctc gccgagcccg cgaccctcga tgcccgcggc gaggtctaca
     3661 tgccgaaggc cgagttcgcc cggatcaacg ccgagcgcga ggaggccggt ttcgccctct
     3721 acgccaaccc ccgcaactcc ggcgccggct cgctccgcca gaagaatccg caggtcaccg
     3781 ccagccgcca gctgagcacg tgggcctacc agctgatcga ggacgaggac gaggacgccc
     3841 gcccggccgc cgccggcgtg gccagccagt cggccgccct cgatcggctc gctgcgctcg
     3901 ggttcgccgt caacccgaat cgggaagccg gcctcgacat cgagggtgtc atcgccttca
     3961 ccgagagctg gcgcgacggt cgccaccacc tgccgtacga gaccgacggc gtcgtcgtca
     4021 aggtggatcg gttcgaccag caggcccggc tcgggatggt cagccgggcg ccccgttggg
     4081 cgatcgccta caagttcccg ccggagcagg tcgagacgat cctcgaggac atcgtcccgt
     4141 acgtcggccg gaccgggacg ctgacccccg tggcccacct caccgcggcg aaggtcgcgg
     4201 gctcgacggt cacgcgcgcc accctccata acctcgacga gatcggtcgc aagggcctct
     4261 tgatcggcga tcacgtgatc ctccagaagg ccggcgacgt gatccccgag gtcgtccgac
     4321 cgatcgtcga gaagcggacc ggcgccgagc ggccgttcgc gatgcccgag cgctgcccgg
     4381 tctgcgcgac gccgatcgtc cgggacgagg gcgcggtccg ccattactgc ccgaacctcg
     4441 cctgcccggc ccgggtgtcg caggagttcc aacacttcgt cgggcggggc gggatggata
     4501 tcgagggtgc cggctggaag gtcctcgagc agctcctcca gacccggctc gtgcagcgcc
     4561 gcggcgactt ctaccggctg tcggtcgagg acctcgaggg tctcgagcgg ttcggccgga
     4621 agagcgccga gaacctccac gccgcgatcc agaagtcgcg ccgacggccg ctcgagcgga
     4681 tcctggccgg gctcgggatc ccccaggtcg gctggacgac ggcggtcgat ctggcggcct
     4741 ggctggccgg cgaggttccg cccggtgacg actggctccg tcgtgccgcg gcccacctcg
     4801 aacgcctcgc gaccgaggag ccggcgcgct tcgaggaggt cgaggggatc ggtccgacgg
     4861 tctcgaccgc gctggccgcc tggttcgccg cggacggccc cggccggggg gtgctcgagg
     4921 acctcgccga cgccggcgtc gagcccgagc tgccgacgcc gcgcccggcc ggcggccggg
     4981 aatcggccgt cgacgggcca ctcgccggca ggaccgtggt ggtcagcggg tcgatcgagg
     5041 gctacagtcg cgacgaggcc gagcaggccg tccgcgctgc cggcggcaag ccgtcgggct
     5101 cggtctcgaa gaagaccgac tatctcgtcg ccggtccggg cgccggttcg aagctcgcca
     5161 gggccgagga gctggcgatc ccggtgctcg acgccgaagg gttcgcgaag ctcctgaggg
     5221 gagaaggatg aacgatgcag aggagcgctt cgcggagcgg ctggcggtcg acctcgagcg
     5281 ggtgctcggc gccgggctgg cggtcgacga catcgagc
//
LOCUS       DATAIW010000516         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_264736, whole genome shotgun sequence.
ACCESSION   DATAIW010000516 DATAIW010000000
VERSION     DATAIW010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264736"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VHM48_15130"
     CDS             complement(<1..452)
                     /locus_tag="VHM48_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756802.1"
                     /translation="MIVAAIALVAGSTLLPYPAAAQTVAPTLTAPSVSPSAPSPDKAA
                     ADTRQRANAGADTDQVVLAFSKVLGASEARSAARLKSLALAAGSPVKFVRLLDSRHAV
                     YRLASRLGDHSGSTLAAMARIPDVARAEPDPVVSVADLPDDAYAPQLWG"
     gene            complement(677..2338)
                     /locus_tag="VHM48_15135"
     CDS             complement(677..2338)
                     /locus_tag="VHM48_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2756803.1"
                     /translation="MAGLNVYRELLGNRALMRLLIGEFISGIGDWLYIVAIFVVIYRE
                     SGDAALVGAFGAIRLLPYVILSVPAGIVADRFDRRIVLLVSDLYRGSLMVVMAVLVAM
                     HGPTLLIAALAILAASGSAFFYPAMGAYLPALARDERQLGPANSAWASIQNFSFIVGP
                     AIAGIVLALGSVLIAFILNAVSFIFIAVILWSLPPSRAGQAVATTTDDETTAEAPATT
                     RAPAEAPLQIRPLAGLTIVQLMAGFLGGGLQVITVILAIDVLKAGEEANGYLNAAIGI
                     GGLIGGIGAGALVLRRRLGAPLLAGALVTGVGTIALGAATTLPVALVAIGVLAAGALI
                     IDVVGTTVFQRLVPDELRGRGVGVLMAVSTLTGAAGAYLLPVMLTTIGPFESLGAAGV
                     ATIAFTVIGLAMIGTAADRAPTPYEATIARVITLPLFTGIPASRLEAALRRVIEVPVT
                     AGEPVVRQGAPADRFYIIESGAFRVTQAGPAGGEPVVLRQLGPDDVFGELGLLNRTPR
                     TATVTATTDGLLLALDRDDFLALVGAAGPLRGRLLGLYSGTTATR"
     gene            2500..3159
                     /locus_tag="VHM48_15140"
     CDS             2500..3159
                     /locus_tag="VHM48_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2756804.1"
                     /translation="MDSSPLRVVIAEDSALIREGIARLIQESGGTVVAKVGDGPSFVS
                     AVVAHHPDVSVVDVRMPPSQRDEGLRAAIEARRLVPGTPVLVLSQYVERQYATELLAD
                     RAGGVGYLLKDRVGDIREFMDALRRVARGGTALDPEVVAQLMVRDHADDPLSALTPRE
                     REVLAAMAEGRTNVGIAALLGITEGATEKHISSIFGKLELPDSQNDHRRVLAVLTYLG
                     S"
BASE COUNT          525 a         1162 c         1158 g          413 t
ORIGIN      
        1 ccccagagct ggggggcgta cgcgtcatca ggcaggtcgg cgacgctgac gacggggtcg
       61 ggctccgcgc gcgccacatc cgggatcctg gccatcgccg ccagcgtcga tccgctgtgg
      121 tcgcccagcc gcgacgcgag ccggtacaca gcgtggcgcg agtcgaggag gcggacgaac
      181 ttcaccggcg agcccgcggc gagcgccagg ctcttgaggc gcgcagcgct gcgggcttcg
      241 gacgccccga gcaccttgga gaacgcgagc accacctggt cggtatcggc acccgcgttc
      301 gcccgttgcc tggtatcggc agcagctttg tcgggggacg gcgcagatgg cgaaaccgac
      361 ggtgcggtca gcgtcggcgc gaccgtctgc gccgccgcag ggtaggggag cagcgtcgac
      421 cccgcaacga gcgcgatcgc cgcaacgatc acgccgaccg accggcgacc cggatggcgt
      481 gccctcacgg tcgcatcgac gccggctgcg acgccgatgg aatcccgtgc gtgttcaagc
      541 tccggtcacc cccagccgtc ggctctgtcc tcagcgaacc atcgccgacc cagggcacct
      601 gcgcaacctg ccgaatgggc cactcgtcag cgctgaatac ctgctgagca cgtggcgctc
      661 agcgagaagt cggttctcag cgcgttgccg tggtccccga atacaggccc aggagacggc
      721 cgcgcagcgg gccggcggcc cccacgagcg caaggaaatc gtcgcgatcg agggcgagga
      781 ggaggccgtc ggtcgtggcc gtcaccgtcg ccgtgcgtgg cgtccggttg agcaggccga
      841 gctcaccgaa cacgtcatcg ggtccgagct ggcgaaggac gaccggctcg ccgccggcgg
      901 ggccggcctg cgtgaccctg aaggcgcccg actcgatgat gtagaagcgg tcggcgggag
      961 ccccttgcct cacgaccggc tcgccggccg ttaccggcac ctcgatgacg cgcctcagcg
     1021 ccgcctcgag acgcgaggcg ggaatgcccg tgaacagcgg caacgtgatc acccgggcga
     1081 tggtcgcctc gtacggagtc ggggcgcgat cggcggcggt cccgatcatc gcgaggccga
     1141 tgaccgtgaa ggcaatcgtc gcgacgccgg ccgcgccgag cgattcgaac ggcccgatcg
     1201 tggtcagcat gacgggtaac aggtacgccc ccgccgcccc ggtgagggtc gacacggcca
     1261 tcaggacccc gacgccgcga ccgcggagct cgtccggcac caggcgctgg aagaccgtcg
     1321 tccccacgac gtcgatgatc aacgcgccgg ctgcgagaac cccgatcgcg acgagcgcga
     1381 cgggcagcgt cgtcgccgcg ccgagggcga tcgttccgac accggtgacg agggcgccgg
     1441 cgaggagcgg cgctccgagg cgtcgtcgca ggaccagggc acccgcgccg atcccgccga
     1501 tcagcccacc gatgccgatc gccgcgttca ggtagccgtt cgcctcctca ccggccttca
     1561 ggacgtcgat cgcgaggatg acggtgatga cctggagccc tccgccgagg aacccggcca
     1621 tgagctggac gatcgtgagg ccggccagcg ggcggatctg caggggagcc tcggcagggg
     1681 cccgcgtggt cgcgggcgcc tcggcggtcg tctcatcgtc ggtcgtcgtg gcgaccgcct
     1741 gacccgctcg gctcggcgga agcgaccaga ggatcaccgc gatgaagatg aaggacacgg
     1801 cgttgaggat gaacgcgatc agcacgctcc ccagcgcgag cacgatcccg gcgatcgcgg
     1861 gccccacgat gaagctgaaa ttctggatgc tggcccaggc gctgttcgcc gggccgagct
     1921 gccgctcgtc ccgagccagg gccgggagat aggctcccat cgccgggtag aagaacgcgg
     1981 agccgctggc ggccaggatc gcgagtgcgg cgatcagaag cgtcgggccg tgcatcgcca
     2041 cgaggaccgc catgaccacc atcagcgagc cgcgatagag atccgagacg agcaggacga
     2101 tccgccggtc gaagcggtcg gcgacgatcc cggcgggcac ggacaggatc acgtagggca
     2161 gcaggcggat cgcgccgaac gcgccgacga gcgccgcatc gccggactcg cggtagatca
     2221 cgacgaagat cgcgacgatg tagagccagt cgccgattcc gctgatgaac tcgccgatca
     2281 gcagacgcat cagcgcccgg ttgccgagca gctcccggta gacgttcagg cccgccactc
     2341 gcgtgttctc cctccgctcg acgcgccccg gcgcccgcat tgagccgccc gatcatagcg
     2401 acccgacgcc ccgtcggatg gccgcctgcg cagtccgccc ggaagggcca ccatccgatc
     2461 cacccggcag cgggccacgc gggcgtgcat gatcgacgcg tggattcctc acccctccgc
     2521 gtcgtcatcg ccgaggactc ggcgctcatc cgggagggca tcgcgcgcct gatccaggag
     2581 tccggcggca cggtcgtcgc caaggtcggt gacggcccgt cgttcgtctc tgccgtcgtc
     2641 gcccaccacc cggacgtgtc ggtcgtggac gtccggatgc cgccgagcca gcgggacgaa
     2701 ggcctccgcg cggcgatcga ggcccgccgc ctggtgcccg ggacgcccgt cctcgtcctg
     2761 agccagtacg tcgagcggca gtacgcgacc gaactgctcg cggaccgagc aggcggcgtc
     2821 ggctacctgc tcaaggaccg cgtcggcgac atccgcgaat tcatggatgc cctgcggcgc
     2881 gtcgctcgcg ggggaacggc actcgatccc gaggtggtcg cgcagctcat ggttcgggac
     2941 cacgccgacg acccgctgtc cgcgctgacg ccgcgcgagc gcgaggtgct ggcggcgatg
     3001 gccgagggcc gcacgaacgt ggggatcgcg gcgctcctcg ggatcaccga gggcgcaacc
     3061 gagaagcaca tcagcagcat cttcggaaag ctcgagctgc ccgattcgca gaacgaccac
     3121 cggcgcgtcc tcgcggtcct gacgtacctc ggctcctagg acctacctcg gctgccagga
     3181 cctagcgggg ctccgggatc ggcagctccg cccggacgac cgttggaccg ccggccgggc
     3241 tcgagaccgt gaaggttc
//
LOCUS       DATAIW010000517        22309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_146204, whole genome shotgun sequence.
ACCESSION   DATAIW010000517 DATAIW010000000
VERSION     DATAIW010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146204"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHM48_15145"
     CDS             <1..255
                     /locus_tag="VHM48_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2756805.1"
                     /translation="IRRIVGQVDLAGMDIVEVSPPYDHAETTAMIANRAALEAISALA
                     LKRRDGRPIRYTGPGTGEGQPSPVVDATGRATNGDAPASG"
     gene            252..1079
                     /locus_tag="VHM48_15150"
     CDS             252..1079
                     /locus_tag="VHM48_15150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2756806.1"
                     /translation="MTSRQTAPADAQPTATSRALARLYDLDLVEDPGDVDLYLELATR
                     TGGPVLELAAGSGRIAVPLALAGHDVTAVDIEPAMIERAFARARAAGRRVSGRLDLIE
                     ADLLELDLPDAGTFRLAFIALNSLFLLATRDAQRQAFRTLARHLAPGGLAVVDVWLPE
                     PEDLARFDGRMIFEYERREPETGHEVTKVATARHDPTTAVVDLTAIYEEGPAGGTPIR
                     WIRHDALRLVGADELRAMAEEAGLVVDEIGGDYDLEPIGPGSERAVLIARRPDAGRA"
     gene            1185..2342
                     /locus_tag="VHM48_15155"
     CDS             1185..2342
                     /locus_tag="VHM48_15155"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2756807.1"
                     /translation="MASSDQTRLLLVEDVPQVAQYIRGLLNSQSSVKLLDVLSDGSNV
                     LAQVQQLRPDVVLVDALLQGRVKGLPLAEEIAKAGYGVPVIVLTVPQNPVSPDPAKGI
                     HGVLAMPFSGFDLMNRIAAVRKDFDKLSTTGSSRIYSVFAPKGGVGKTTIAFNLAVAL
                     GQAEQRTVLIDGSLQFGDLRSLLRVPVDAPSILDLPTDRVAESDLQDVLWRDPSGIDI
                     LLAPPRVEMAEMVTARDIDKILSLLRRVYGSIVIDMSSALNDINLSFLDLSDTIVEIV
                     TYDSTTIHNTIAVADAFRMIGYPASKVRYLVNRSDSPGGIDPGDLERAIGRVPEHRVV
                     SDGLLVVQSNNEGVPFVLANPAAQISQDVHRVAADLLAANGIVRPAAAGRR"
     gene            2352..3272
                     /gene="murI"
                     /locus_tag="VHM48_15160"
     CDS             2352..3272
                     /gene="murI"
                     /locus_tag="VHM48_15160"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX2756808.1"
                     /translation="MSDPRPIGVFDSGVGGLTVLREVLRRSPHESTIYLGDNLRAPYG
                     IRTDTEVRAFSIEALDELAARDVKAIVVACNTSTAVALPEFRRRYDLPILGVVRPGAV
                     TASLTTRNRRVGVIATPATVRSHAYFNAIKDENPAVEVYEHATPAFVPLVEAGRIGGA
                     EVEAIVAESLAPLLGERDQAGEFVFPLPASARIDTLLLGCTHYPLLRPVIAAIAGERV
                     AIVDSATATASALVELLAINGLEAPGTTRGTAADAGAAGHERPDEVAATATHIQLTTG
                     DIDAFRAVAERIFGEAFPDVGSVEVSAVAS"
     gene            3289..4509
                     /locus_tag="VHM48_15165"
     CDS             3289..4509
                     /locus_tag="VHM48_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2756809.1"
                     /translation="MSGTRNKGNGWRDDRVWQAGFLVGSALGAAATVLGRRAERVARQ
                     GLVDWPAAERYAVARLRSAPGALTAAELRASEPSYAAAMELIVPRLSAALGTDLPGVV
                     ERSAVVDRAGWVHANVATFAQLIGQLETELLDQVVPHGGGLAKATMALANRWVTTRQL
                     GFLLGFMGSKVLGQYDLALLTAEATPGKLLFVEENIRATATALDVPLEPFRTWIALHE
                     TTHAFEFEAHPWLRPYLADRLERQLKLFGKEASGLGREALRSLGRAIRGESDGVEHWM
                     ERLMTAEQRTLFRETQAVMSLLEGFSDYVMDEVGKDLVPGVERISTKFHERRQARRSP
                     FERAVMRLTGMDVKLEQYARGEAFVSAIAAAGGPVALNRLWDGPDTLPRPGEIDNPAA
                     WIQRVGGTASTAAS"
     gene            4506..5573
                     /locus_tag="VHM48_15170"
     CDS             4506..5573
                     /locus_tag="VHM48_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX2756810.1"
                     /translation="MSDEVRRRMAGPGSGPGGAPTAIALSPILSARYRSRDLELIQAA
                     APGARIVTLSREGLTDEPVEDVEVLLRGWLSSDAFDRLLARAPRLNYVHSASAGVERA
                     LTPAALERGLVITNARGVFSRPIAEYVLMMILAVSRRLPGLLELQRERTWQPLEGVEL
                     RDVTVGIVGLGSIGRAVGALATAFGCRVVAVRRRSEGGGPTMATDDDTRSFGEVMLDR
                     VGGPESLPELLAESDFVVLAAPLTPETEDMIDEAALGAMKRSAWLINIARGKLVDERA
                     LLDALREGRIGGAVLDTFREEPLPPTSAFYDLENVIVTPHTSWSSGRVLDRSVELFCD
                     NLRRYAAGEPLLNVVDPTAGY"
     gene            6072..7175
                     /gene="ychF"
                     /locus_tag="VHM48_15175"
     CDS             6072..7175
                     /gene="ychF"
                     /locus_tag="VHM48_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX2756811.1"
                     /translation="MQIAIVGLAGSGKTTVFNTLTRGHADTGGFGGMELHVGVVKVPD
                     ERLAKLAAIFKPKKIIQADVTYVDLPAPPASTEGHVGTEDLPAEHLARLRDSDALLHV
                     VRAFEDANHPHPEGSVDPARDLERLDVEFILADLALVDRRLERLKQSGRHGTPAEKEA
                     NEREEAVLVRLKAGLETGAPIRDLGLDPDEEKVIRGFRFLTQKPVLVLLNVGEGDIAG
                     AGERAATLAAGYDHRSALVDALSARIEMELGELEPDEAAVFMEELGIAASGLDRVIEL
                     SYRLLGLISFLTAGPDEVRAWPIRDGSNAVDAAGAIHSDLARGFIRAETVAYEDLLKL
                     GSMAAARKVGKLRSEGKTYKVHDGDVVEILFSK"
     gene            complement(7441..7821)
                     /locus_tag="VHM48_15180"
     CDS             complement(7441..7821)
                     /locus_tag="VHM48_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756812.1"
                     /translation="MYFFELHEGDEDVFSDVLLVREEEMDPDEFFELVESVRHQVQDT
                     YTDDTLIEAIAAELEREHDFLFVSDNRIVAAVNVSPIDEENFLVAIDTDEEAAEDERG
                     ADYRAVIAEFDPHGEDVRGEDDRG"
     gene            7959..8891
                     /locus_tag="VHM48_15185"
     CDS             7959..8891
                     /locus_tag="VHM48_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2756813.1"
                     /translation="MVPTVVIVGAASRDLADDDPRGWRLGGGVSYSALTTARLGLPTA
                     AIVGVDAEAATASELDLLRDAGVDILLVHLDHGPVFVNIERPEGRLQLCGDHSDPVPV
                     AAVPAAWRSAPGWILAPVAAELPPAWADVPAEEAIVAVGWQGLLRELDAGQPVRQVAP
                     RPDPIIRRADLVGLSRDDVDRAIPLADLYGLLRRGASLAVTQGDHGGLVVHGAHGGHE
                     AALELRHYPAVPSHAAVDPTGAGDVFLAALAAARIEPRLVGGRIGQGFDLLLAAAAAS
                     LVLEGPGMFGVPTRDAVRTRMAEALQRRSAAAEA"
     gene            complement(8966..10009)
                     /locus_tag="VHM48_15190"
     CDS             complement(8966..10009)
                     /locus_tag="VHM48_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2756814.1"
                     /translation="MATTIADVARRAGVSTATVSRVLSGIGRARPETHARVEAAAREL
                     GFRPSDVARSLKRRSTLTLGLIVTDIENPYFPQLVRAVEDAASGEGYAILLCNADDDP
                     EREASYLDFLVERRVDGLIIAASTIGMRQGEWLATASLPVVLINTAATGDPQPTIVSD
                     NRGGGSAAARHLLDFGHRRFGYLMPPPRNLDAPERLAGVHDALDSAGLSWEGPGSALA
                     IGHGAPTVTGGESAMSELLERAPRPTAVIAYNDLMAIGAMRAIRTSGFRIPTDISLIG
                     FDDVAFAAYVEPALTTLRQETAEMGRWAVATVTERIKAAGRDGSRPIATGGRRVVPVH
                     LEIRASTGPAPDS"
     gene            complement(10016..10942)
                     /locus_tag="VHM48_15195"
     CDS             complement(10016..10942)
                     /locus_tag="VHM48_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2756815.1"
                     /translation="MTMSPSAAGPAPTAAAARAARKPLLEARNISKYFGAVVALEGVS
                     LTVRAGEINCLLGDNGAGKSTLIKTLSGVHPPDDGTLAVDGEPVIFSSPRAALDAGIA
                     TVYQDLSVMPLMSISRNFFLGNEPTKGFGPFRRFDSAKADAIAREEMHNIGIDVRDTE
                     QLVGTLSGGERQTLAIARAEYFGARVLILDEPTSALGVKESAFVLRHAIAARNKGLGV
                     IFITHNVQHALPIGDSFTILTRGRSGGTYRRGELTVEQLHGLMGGGSELEELQADLEM
                     LARREEAGEDVPTVPLGTDPDSAPILGENQHS"
     gene            complement(10960..12039)
                     /locus_tag="VHM48_15200"
     CDS             complement(10960..12039)
                     /locus_tag="VHM48_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756816.1"
                     /translation="MATTTADAAPTPAPERLRQVSLLQRLLAKPAAGAFVIVVFVFVV
                     FAVLAGLKGTNAFLSIPGVLNYVGVASQIGIIATSVSLLMIAGEFDLSVGSLVGFSGI
                     IIGICASVWGLPIWLSIIISMILTTGLGVLNGLIVVRTGLPSFIVTLATLFIIAGLSS
                     VLVLSATNITFISIDKAAIAADPLAQFFNWKLSFKFDLATSDLYVSVVWWILIAIVGA
                     YVLGRRRFGNWIAGVGGSPVAARNLGVPVARVKILLFASTAFSASILAACQSMQFASA
                     DIQRGTGYELTAITTAVIGGCLLTGGYGSVAGTALGALALGMANIGIVYASINADWYK
                     IAIGLLLLTAVVLNNWIRRRFAGLR"
     gene            complement(12128..13144)
                     /locus_tag="VHM48_15205"
     CDS             complement(12128..13144)
                     /locus_tag="VHM48_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2756817.1"
                     /translation="MNKRTLFTGLAGIAIVLSACNSTPAASGATTGAKGPDGALVKRA
                     DIKIEVVTHGQAIDGFWGVVRNGVKAGAADMGVTVNYNAPAQESDMPGMSSLIDAAVA
                     KKPSGLVVSIPNPDALSPAIKKAVEAGIPVVSMNSGSDVFKGLGILAHVGQTELQAGI
                     GAGERFKAAGAKNVVCFNQEVGNQALTLRCNGLFQGMGLTDPSQGKVLTGKISDPTGM
                     QATIAAAIQSDPTIDGLLTLGPSVAGPALLAVTASGKPIHLATFDLSADVLTAIKDGK
                     MDFAIDQQQFLQGYLPIVILTNYAETKNLPTGDGTGLIMTGPGFVTKDNAAAVIALAA
                     KGLR"
     gene            complement(13321..14322)
                     /locus_tag="VHM48_15210"
     CDS             complement(13321..14322)
                     /locus_tag="VHM48_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2756818.1"
                     /translation="MKVALLGAGRIGRLHARLLSATPGVDELVIADALPERATEAAAE
                     VNGRAAPSLEAALDEADAVVIAAATTAHAELIRASADRGRPTFCEKPLAEDITATIEV
                     ARHIEKSGIPFQLGFQRRFDAGYGEAHRMIESGELGTLYNFHLVGHDPEPPHEGYIPQ
                     SGGLFRDFSIHDFDVIRWLTGHEVVEVYADGDVRGFPMFAKYNDVDTAVATLRMDDGA
                     LGVLTVARHDPLGYDIRAELFGSKDSISVGLGPRTPIRSVEPGVPPQAGPAWPHFLDR
                     FSAAYSAEFAAFVRVARGDEPSPCTAHDGVQALRIAEAATQSLHEHRPVRVEEIANS"
     gene            complement(14319..15332)
                     /locus_tag="VHM48_15215"
     CDS             complement(14319..15332)
                     /locus_tag="VHM48_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2756819.1"
                     /translation="MITVAPSTAVVEQVARLRRRLAAGGPLGGASIGTVPILWNNVDI
                     AELRLGTSAETILDDIARTAYDGCQLGLGFPEGDDLKATLAARDLRLAEVYASIPATV
                     DGPTATALDEVRERIRLLRAGGGEVLCIAFDGSPDRSPFAGRAADPATPRLTDEGWTR
                     TIELLETVSAETRAAGARIAFHPHAGTYIETPEEVERLASSIDPTVLPFCLDVGHYLV
                     GGGDPVTALRRYGERVEHVHLKDVDPDVLDGLRHGTAGGFGGAIRARLFTELGAGALD
                     LDGVLGVLAERRYAGWLMVEQDSGWPPPAESAAIGRRVLAAALRRLAGTGDAAATTEG
                     APR"
     gene            complement(15329..17152)
                     /locus_tag="VHM48_15220"
     CDS             complement(15329..17152)
                     /locus_tag="VHM48_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX2756820.1"
                     /translation="MSDSVTALSRLTGAQILVEYLVRQRVPFAAGIPGHGCWAITDAL
                     LDRQDVIRTIQVMHEQSAVHLADGYFRATGQPMLAFTSIGPGAMNTVVGMGTAYVDST
                     AVALAIGSPHTYMRGHGLFQEFERRHHADNPRVFEPVVKEWWQPSRVDDLPFVLHRAW
                     NAMTSGRPGPILLDLPMDIQAEAADVRMPEPDQREARGRIRPAADDVERAAASLRGAL
                     RPVIVAGGGVLLAEAWDEVRALAERLGAPVVTTWNGKGAIDETHDLSAQTIGDTASTC
                     GNLLSASADVVLSVGQRFTDWSASSYRKGVTFAIPPSRLIQVDIDPREIGKNYPVDVA
                     LVGDAKAALGDLLAALGPGGGPAAYRATAYFAEIQQLKADWFDQVEIKSASRATPMTM
                     ARAVREVQRATSDDAVVVTGAGLPQGMVKQRWVTRHPRTHLTSGGFSTMGFTLPAAIG
                     AQLALPDREVVAVCGDGDFLQTMQELQAAVLAGTPVCTIVLDNSGWISIKGGQENFFG
                     RTAWTDFVTPDGSIYSPDFAAIGAAFGIHSEAATHPDEVEPAVRRALASGGPSLVHVK
                     VDRDLAVAGPDKTGWWDAPSPPHHPEQHARWLAGRAEEQHQ"
     gene            17404..18957
                     /locus_tag="VHM48_15225"
     CDS             17404..18957
                     /locus_tag="VHM48_15225"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2756821.1"
                     /translation="MTQVAEPGSARAQDEANGTATGAVHHWIGGRIVEGTSGRRGPVY
                     DPATGRIAREVDFASVDEVAAAVAAAKAAFPAWRATSLSKKTDILFKIRNLVEAHRKE
                     LAAHLTAEHGKVPSDALGEIARGIENLEFACGIPNLLKGGFSEQVSGGVDVFQIHQPL
                     GVVAGITPFNFPAMVPMWMFATAIATGNTFILKPSEKDPSAANFMADLLHEAGVPDGV
                     FNVVHGDKVAVDALLEHPDIAAISFVGSTPIARYIYETGTKNGKRVQALGGAKNHMIV
                     LPDADIDMAADAAISAGYGSAGERCMAIATIVAVGDVADPLVEAITKRLPKVKVGPGS
                     DPEAEMGPLVTRQHRDKVASYLDSSAAQGATVVADGREHPLFKDSDGFFLGVSLIDYV
                     STEMDAYKDEIFGPVLTIVRVPTYEEAVRLINDNPYGNGTAIFTRDGGAARQFQFEVN
                     AGMVGINVPIPVPVAYYSFGGWKSSLFGDLHMYGPEGIQFYTRAKIVTQRWPDPGTSK
                     IDLGFPRTR"
     gene            18957..20219
                     /locus_tag="VHM48_15230"
     CDS             18957..20219
                     /locus_tag="VHM48_15230"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2756822.1"
                     /translation="MAALDPAPGSATGRNGQVFIVSAARTPIGKFGGVFADTPATTLG
                     GVAIRAAMERSGLPLDTPVDEVLMGQVLQAGVGQAPARQAALSAGLADTTSATTINRV
                     CGSGLKAIMLAAAEIRAGDAEIVVAGGMENMNLAPFLLPKARFGYRLGNATLVDAAVA
                     DGLTCVFEGCHMGTHAERVAIHDHISREDQDAFALASQQRAIAAQDAGRFAAEMAPVT
                     IHDAKGRETVVEVDEGPRRDSSLEALARLKPVFDLPTGEDRGDASVGTVTAGNAPGIT
                     DGAAATIVVSERAVDRFGLTPLARIVGYTQAEIAPKWLFLAPITGVRRLLDRIELPIE
                     AFDLIEINEAFAAQTLADGRELGFDWDKVNVNGGAIALGHPIGASGARIVATLLHELA
                     RREGRYGLATLCLGGGGSVAMALERVVA"
     gene            20248..21363
                     /locus_tag="VHM48_15235"
     CDS             20248..21363
                     /locus_tag="VHM48_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX2756823.1"
                     /translation="MTLIDSTAPALADTPLGRTIATTATDIWNDSCAIDELEYAISFG
                     AVGATANPTIVTDVWKREPARWKARVAALAVERPDASEVDLAWQVVDEMSTRAAPLLL
                     PAFEAHGGRQGRLSTQTDPTFYRSAERMLAHSLHFDSLAPNIIVKFPATSAGIDAMEE
                     ASARGVSVNTTVCFSVAQALAAAEAIERGMRRREAAGESTAAMGPVVTIMVGRVEDWL
                     RVQTERDGIIADPAALPWSGVAVFKRAYGLFRERGYRPRLLAAAIRHHYHWSEFIGGD
                     IVITLPAAWQKRFNASSIEVRPRMDDPVDPRIVAELAGRYPDFVRAYEPDGLRRDEFD
                     AFPPTARTLRAFIASYHELLHQVTDAMIPNPDLRPAS"
     gene            21369..22289
                     /gene="iolB"
                     /locus_tag="VHM48_15240"
     CDS             21369..22289
                     /gene="iolB"
                     /locus_tag="VHM48_15240"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX2756824.1"
                     /translation="MPEPTIHTRALPSELAPADLRLRPDPDGAVSLDPTRAGWRYLSF
                     AVAAVPPGGLRIPAAQDMETCVVVQRGHGTTIRAGDRHWSLPGREDVFADLPSALWLP
                     DGRALTVEVERRPAVGPALVAVARAPRSGRDGVAAGLDHDIVGHDRDGLRGAGNATRQ
                     ISHIIAPTFPADRLEVVEVYTPSGNWSSWPPHKHDVDDMPGEAVLEEIYHYQFRRPEA
                     WAIQRVYRLDRSRDALFEVRHGDAVIVTDGYHPFSATHGDDAYYLNALAGDRRTMACS
                     FDPDLDWVRATWAGMELDPRVPVVPPRRAG"
BASE COUNT         3213 a         7850 c         7977 g         3269 t
ORIGIN      
        1 atccgccgga tcgtcggcca ggtggatctt gcgggcatgg acatcgtcga ggtctcgccg
       61 ccgtacgacc acgccgagac gacggcgatg atcgccaacc gggcggcgct cgaggcgatc
      121 agcgcgctcg ccctcaagcg ccgcgacggg cggccgattc gctacaccgg gccgggcacc
      181 ggcgaggggc agccgagtcc ggttgtcgat gcaacgggcc gggccacgaa cggcgacgcc
      241 ccggcgtccg gatgacctcg cgccagaccg cgcccgccga cgcccagccg acggcgacga
      301 gtcgggccct ggcccggctc tatgatctcg acctcgtcga ggacccgggc gacgtcgatc
      361 tctacctcga gctcgcgacc cggaccgggg ggccagtcct cgagctcgcc gcgggcagtg
      421 gccggatcgc cgtcccgctc gcgctcgccg gtcacgacgt caccgccgtc gacatcgagc
      481 cggcgatgat cgagcgggcg ttcgcccggg cccgggccgc aggccgtcgc gtcagcggcc
      541 ggctggatct gatcgaggcc gacctgctcg agctggacct gcccgacgcg ggcacgttcc
      601 gacttgcctt catcgccctg aactcgctgt tcctgctcgc cacgcgagat gcccagcggc
      661 aggcgttccg gacgctcgcc cggcacctcg ccccgggcgg ccttgcggtc gtcgacgtgt
      721 ggctgcccga gcccgaggac ctcgcccgct tcgacggccg gatgatcttc gaatacgagc
      781 gtcgagagcc cgagaccggc cacgaggtga ccaaggtcgc gaccgcccgc cacgacccga
      841 cgaccgccgt cgtcgacctg accgcgatct acgaggaggg cccggccggc gggaccccga
      901 tccgctggat ccgccacgat gcgctgcgtc tggtcggcgc ggacgagttg cgggcgatgg
      961 ccgaggaagc cgggctggtc gtcgacgaga tcggcggcga ctacgacctc gaaccgatcg
     1021 ggccgggcag cgaacgggcc gtcctgatcg cgcggcgccc ggacgccgga cgagcgtgac
     1081 ggagcatcgg gcagcccgga tccgggtcga cgccgctgcg ccggcaggcg atgggacttc
     1141 cgggtccgat gccacctccg gctggcttgg tacactcggc cacgatggca tcgagtgacc
     1201 agacgcgcct cctcctcgtc gaggacgtcc cccaggtggc gcagtacatc cgcggactgc
     1261 tcaattccca gagctcggtc aagctgctcg acgttctgag cgacggcagc aacgtcctgg
     1321 cccaggtcca gcagctgcgc ccggatgtgg tgctcgtcga tgccctcctg cagggacgtg
     1381 tgaagggcct gccgctcgcc gaggagatcg ccaaggcggg ctacggcgtg ccggtcatcg
     1441 tgctgacggt gccccagaac cccgtctcgc ccgatccagc gaaggggatc cacggcgtcc
     1501 tggcgatgcc gttctcgggc ttcgatctga tgaaccgcat cgccgcggtc cgcaaggact
     1561 tcgacaagct ctcgacgacg gggtcgtcgc ggatctactc cgtgttcgcc cccaagggcg
     1621 gcgtcggcaa gaccacgatc gcgttcaatc tcgcggtcgc cctcggccag gccgaacagc
     1681 ggacggtgct gatcgacggc tcgctccagt tcggggacct gcgctcgctg ctgcgggtgc
     1741 ccgtcgacgc tccgtcgatc ctcgacctgc cgaccgaccg cgtcgccgaa tcggacctcc
     1801 aggacgtcct ctggcgcgac ccgtccggga tcgacatcct gctcgcgccg ccgcgggtcg
     1861 agatggccga gatggtcacc gcccgcgaca tcgacaagat cctgtcgctc ctccgccggg
     1921 tctacggctc gatcgtgatc gacatgtcgt ccgccctcaa cgacatcaac ctctcgttcc
     1981 tcgacctgtc ggacacgatc gtcgagatcg tgacctatga ctcgacgacg atccacaaca
     2041 cgatcgcggt cgccgacgcg ttccggatga tcggctaccc cgcctcgaag gtccgctacc
     2101 tcgtcaaccg ctccgactcg cccggcggga tcgacccggg cgacctggag cgggcgatcg
     2161 ggcgcgtccc ggagcaccgc gtcgtcagcg acggcctcct cgtcgtccaa tcgaacaacg
     2221 agggcgttcc cttcgtcctc gccaatccgg ccgcccagat cagccaggac gtccaccgcg
     2281 tggccgccga tctgctggcg gcgaacggga tcgtccgacc ggcggcggcc ggcaggcgct
     2341 aggcgggtcc gatgtccgac ccgcgcccga tcggcgtctt cgactccggg gtcggtgggc
     2401 tgacggtcct ccgcgaggtc ctccgccgct cgccccacga atcgacgatc tatctcggcg
     2461 acaaccttcg cgctccgtac ggcatccgga ccgacaccga ggtccgggcg ttctcgatcg
     2521 aggcgctcga cgagctcgcc gctcgcgacg tcaaggcgat cgtggtcgcc tgcaacacct
     2581 cgaccgcggt cgccttgccc gaattccggc ggcgctacga cctgccgatc ctcggggtcg
     2641 ttcgaccagg ggcggtgacg gcctctctca ccacgcgcaa ccgccgggtc ggggtcatcg
     2701 ccacccccgc gaccgtccgc tcgcatgcct acttcaacgc gatcaaggac gagaaccccg
     2761 cggtcgaggt ctacgagcat gcgaccccag ccttcgtccc gctcgtcgaa gccgggcgga
     2821 tcggcggggc ggaggtcgag gcgatcgtcg ccgagtcgct ggcgccgctc ctcggcgaac
     2881 gcgaccaggc gggcgagttc gtcttccccc tgccggcgtc ggcccggatc gacacgctgc
     2941 tcctgggctg cacccactac ccgctcctgc ggccggtgat cgcggcgatc gccggcgagc
     3001 gcgtcgcgat cgtcgactcg gcgacggcca ccgcgtccgc gctcgtcgag ctgctcgcga
     3061 tcaacgggtt ggaggcgccg ggcaccaccc gtggcacggc cgccgacgcg ggcgcggccg
     3121 gtcatgagcg ccccgacgag gtcgcggcca cggcgacgca catccagctg accaccggcg
     3181 acatcgacgc cttccgggcg gtcgccgagc ggatcttcgg ggaggcgttc cccgacgtcg
     3241 gctcggtcga ggtgtcggcg gtggcgtcgt gaacggggag acgacccagt gagcggaaca
     3301 cggaacaagg gcaacggctg gcgcgacgac cgggtctggc aggcgggctt cctcgtcggt
     3361 tcggccctcg gcgccgccgc gaccgttctc ggccggcgcg ccgagcgggt cgcccgtcag
     3421 ggtctcgtcg actggcccgc cgcggagcgc tatgccgtcg cgcggctgcg gtccgcgccc
     3481 ggtgccctga ccgccgctga gctgcgggca agcgaaccgt cctatgcggc tgccatggag
     3541 ctcatcgtgc cccgcctgtc ggccgctctc gggaccgacc tgccgggcgt cgtcgaacgg
     3601 tcggccgtgg tcgaccgggc gggctgggtc cacgccaacg tggcgacgtt cgcccagctg
     3661 atcggccagc tcgagacgga gctgctcgac caggtcgtgc cgcacggcgg cggtctcgcg
     3721 aaagccacga tggccctcgc caaccgctgg gtgacgaccc gccagctcgg gttcctcctg
     3781 ggcttcatgg gctcgaaggt cctcggccag tacgacctcg ccctcctgac cgcggaggca
     3841 accccgggca agctcctgtt cgtggaggag aacatccggg ccacggcgac cgcgctcgac
     3901 gtcccgctcg agccgttccg gacctggatc gccctgcacg agacgaccca cgccttcgag
     3961 ttcgaggccc acccctggct ccggccctac ctcgccgacc gtctcgagcg ccagctgaag
     4021 ctgttcggca aggaggccag cggactcggg cgcgaagcgc tgcgcagcct cgggcgggcg
     4081 atccgcggcg agagcgacgg tgtcgagcac tggatggagc ggctgatgac cgcggagcaa
     4141 cggaccctgt tccgcgagac ccaggcggtg atgagcctgc tcgaggggtt cagcgactac
     4201 gtcatggacg aagtcgggaa ggacctcgtc ccgggcgtcg agcggatcag caccaaattc
     4261 cacgagcggc gccaggctcg tcgatcgccg ttcgagcggg cggtcatgcg cctgaccggc
     4321 atggacgtga agctggagca gtacgccagg ggtgaggcat tcgtctcggc gatcgcggcg
     4381 gccggtggac cggtcgcgct gaaccgcctg tgggacggcc cggacacgct gccccgcccg
     4441 ggcgagatcg acaacccggc cgcctggatc cagcgcgtcg gcggcaccgc gagcacagcg
     4501 gcctcgtgag cgacgaggtc cgtcggcgga tggccggccc gggatcgggc ccgggcggtg
     4561 cgccgaccgc gatcgccctc tcgccgatcc tgtcggcccg ctaccggtcg cgcgacctcg
     4621 agctgatcca ggcggcggcg ccgggcgcgc ggatcgtgac cctgtcgcgc gaaggcctga
     4681 ccgacgagcc ggtcgaggat gtcgaggtcc tgttgcgcgg ctggctgtcg tcggatgcgt
     4741 tcgaccggct gctggctcgc gccccgcggc tcaattacgt ccactccgcg tcggccgggg
     4801 tggagcgggc cctgaccccg gcggccctgg agcgcggcct cgtgatcacg aatgcccgcg
     4861 gagtcttctc gcggccgatc gccgagtatg tcctgatgat gatcctcgcg gtcagccgcc
     4921 gattgcccgg actcctcgag ctccagcggg agcggacgtg gcaaccactc gaaggcgtcg
     4981 agcttcgcga cgtgaccgtc gggatcgtcg gcctcggatc gatcgggcgg gcggtcggcg
     5041 ccctggccac ggccttcggc tgccgggtcg tcgcggtccg gcgccggtcg gagggtggcg
     5101 gcccgacgat ggcgaccgac gacgacacgc gctccttcgg cgaggtcatg ctcgatcggg
     5161 tcggtggtcc cgagtcgctg ccggagctcc tcgccgaatc ggacttcgtc gtcctcgccg
     5221 cgccgctgac gccggagacc gaggacatga tcgacgaggc ggcgctcggg gcgatgaagc
     5281 ggtcggcctg gctcatcaac atcgcccgcg gcaagctggt cgacgaacgg gcgctcctcg
     5341 acgcgctgcg cgaggggcgg atcggcggag ccgtcctcga cacgttccgc gaggagccgc
     5401 tgccgccgac gtcggcgttc tacgacctcg agaacgtgat cgtcacgccg catacctcgt
     5461 ggtcgagcgg ccgcgtcctc gatcggagcg tcgagctgtt ctgcgacaac ctccgtcgct
     5521 acgccgccgg cgagccgctc ctcaacgtgg tcgatccgac cgccgggtac tgagccggcc
     5581 cggccctggg gtcccggcgt gcattcagtg aacctatgcg ccgaggtcga ggtcggcccg
     5641 ggcgttcacg tccgggaaat cgatcatcca atgcacggaa tcgacaagat cggcgggatt
     5701 ccggcccatt ccgagcccct tgggccatcg cggccgctcg agtcgtcgca tgggttcatc
     5761 tgatgcaccg gaatcccgga acggtgcatc caatgggcca ggatcgacgt cgccacgcga
     5821 atcggaggtc gggttcgtcc agcgtccggg tcaagggaaa ccgaaaccga ggatcgagcc
     5881 gtcggcgtcg gcgatctcac cggacccggg tcgtccgccg ggccggggcc acagccgacg
     5941 acgagcagca ccgccagcgc gatgccggtg gtcctcgctc cgcccatcat ccagccacaa
     6001 cccgggtttg cgatcgtcgg cccaggaccg acccgttacg gcacggtcgc gatcccggat
     6061 agcatcgcac catgcagatc gccatcgtcg gtcttgccgg atcgggcaag acgaccgtct
     6121 tcaacacgct cacccggggc cacgccgaca ccggcgggtt cggcggcatg gagctccacg
     6181 tcggggtcgt caaggtcccg gacgagcggc tcgccaagct cgccgccatc ttcaagccga
     6241 agaagatcat tcaggccgac gtcacctacg tcgacctgcc ggccccgccg gcctcgaccg
     6301 agggccacgt tggcacggag gacctgccgg ccgagcatct cgcccgtctt cgcgactcgg
     6361 acgccctgct ccacgtcgtc cgcgccttcg aggacgcgaa ccacccgcac ccggagggat
     6421 ccgtcgaccc ggcccgcgac ctggagcggc tcgacgtcga gttcatcctc gccgacctcg
     6481 ccctcgtcga tcgccgtctc gagcgcctca agcagagcgg ccgccacggg acgcccgccg
     6541 agaaggaagc gaacgaacgc gaggaggcgg tcctcgtccg gctgaaggcc ggcctcgaga
     6601 ctggcgctcc gatccgggac ctcggcctcg atcctgacga ggagaaggtg atccgcggtt
     6661 tccgcttcct gacccagaag cccgtcctcg tcctgctcaa cgtcggcgag ggcgacatcg
     6721 ccggggcagg tgagcgggct gccaccctgg cggccggtta tgaccaccgc agtgccctgg
     6781 tcgatgccct ctccgcccgg atcgagatgg agctcgggga gctggagccc gacgaggcgg
     6841 ccgtgttcat ggaggagctc gggatcgcgg cgtccggtct cgaccgggtg atcgagctca
     6901 gctatcgact cctcggcctg atctcgttcc tcaccgcggg acccgatgaa gtccgggcct
     6961 ggccgatccg cgacggctcg aacgcggtcg atgcggccgg ggccatccac tccgatctcg
     7021 cccgtggctt catccgggcc gagaccgtcg cctacgagga cctcctgaag cttggctcga
     7081 tggccgcggc ccggaaggtc ggcaagctcc gttcggaagg caagacgtac aaggtccacg
     7141 acggcgatgt cgtcgagatc ctcttcagca agtagaggcg agccttgggt cggcgggcgt
     7201 cggcgcccga ctgccaaccg gatgcccagc aggctcatcc gacatcggaa tcgagccgcg
     7261 actgccgttt catgcctccg gcgaatggat cgtgatcaat cggtgctgaa acccccacgg
     7321 gcatggatcg accgcatgat tcgtcggtgg ccgatcccgg ctgaacgagg aataggcctt
     7381 ctcggcatat tggccggctc ggcatattgg cccgcctgag cagccgtcgg gcgccgagga
     7441 tcagccccgg tcgtcctcgc cgcggacatc ctcgccgtgc gggtcgaact cggcgatgac
     7501 ggcccgatag tcggcgccgc gctcgtcctc ggccgcttcc tcgtcggtgt cgatcgcgac
     7561 gaggaagttc tcctcgtcga tcggcgagac attgaccgcg gccacgatcc ggttgtcgga
     7621 gacgaagagg aaatcgtgct cgcgctcaag ctcggctgcg atcgcctcga tcagcgtatc
     7681 gtccgtgtag gtgtcctgga cctggtgacg gaccgactcg accagctcga agaactcgtc
     7741 gggatccatc tcctcttcgc ggacgagcag gacatcggag aagacgtcct cgtcgccctc
     7801 atggagttca aagaagtaca cgccggcctc ggtcggaaag gggttgggat ggacgttcag
     7861 tatcgcatgc ggactggcgc gcgaagcccg acacgcttgc ggagcgggcg ttgacggagc
     7921 gggccgtgga ggctggtgga cttcccgacc gacccgcgat ggtcccgacg gtcgtcatcg
     7981 tcggtgccgc gtcgcgcgac ctcgccgacg acgatccgcg cggctggcgg ctcggcggcg
     8041 gcgtctcgta cagcgccctg accacggccc gtctcggcct cccgaccgcc gcgatcgtcg
     8101 gggtcgacgc cgaggcggcg acagcgtcgg agctggacct cctccgcgac gcgggcgtcg
     8161 atatccttct ggtccacctc gaccacggcc cggtcttcgt caacatcgag cggccggagg
     8221 gtcggctcca gctctgcggc gatcactcgg acccggtccc ggtcgctgcg gttcccgcgg
     8281 cctggcgcag tgcacccggc tggatcctcg ctccggttgc cgcggagctg ccgccggcct
     8341 gggccgacgt ccccgccgag gaggcgatcg tcgccgtcgg ctggcagggc ctgctgcgcg
     8401 agctcgacgc cggccagccg gtccggcagg tcgcgccccg gccggacccg atcatccgcc
     8461 gggcggacct cgtcgggctg tcacgtgacg atgtcgaccg ggcgatcccg ttggcggacc
     8521 tgtatggcct cctccggcgc ggggcgtcgc tcgcggtgac ccagggcgat cacggtgggc
     8581 tcgtcgtcca cggggcgcac ggcggccacg aggcggcgct cgagctgcgc cactatcccg
     8641 ccgtgccgag ccacgcggcg gtcgacccga ccggcgccgg cgacgtcttc ctcgccgcgc
     8701 tggccgccgc ccggatcgag ccgcgcctcg tcggcgggcg gatcggccag gggttcgacc
     8761 tgctcctggc ggccgccgcg gcatccctcg tcctcgaagg acccgggatg ttcggcgtcc
     8821 cgacccgcga cgcggtccgg acgaggatgg cggaggcgct ccagcgacgt tcggcggccg
     8881 ccgaggcctg agccggcagg gcgggctggg cggcgcctgg gcggcgcctg accgacggcc
     8941 ccggccagat cgtccggcgg cggcgtcagg aatcgggtgc gggaccggtc gaggcgcgga
     9001 tctcgagatg gaccggtacg acccggcgcc cgcccgtcgc gatcggccgg ctgccgtcac
     9061 ggccggccgc cttgatccgc tcggtcacgg tggcgaccgc ccaacggccc atttccgcgg
     9121 tttcctggcg gagcgtcgtc agggccggtt cgacgtacgc agcaaatgcg acatcgtcga
     9181 agccgatcag gctgatgtcg gtcgggatgc ggaagccact cgtccggatc gctcgcatcg
     9241 ccccgatcgc catcaggtcg ttgtaggcga tcacggccgt tggccgtggc gcccgctcca
     9301 ggagctcgga cattgccgac tcgccaccgg tcaccgtcgg cgccccgtgc ccgatcgcca
     9361 gggcggaacc ggggccttcc cagctcaacc cggcggaatc gagcgcgtcg tggactccgg
     9421 ccagccgctc gggggcatcg aggttccgcg gcggcggcat caggtagccg aaccgacggt
     9481 gcccgaagtc gagcaggtgg cgcgcagccg ccgacccgcc gccccggttg tccgacacga
     9541 tcgtcggctg cgggtcgccc gtcgcggcgg tattgatcag gacgaccggc aaggacgccg
     9601 tcgcgagcca ctcgccctgg cgcatgccga tcgtgctggc ggcgatgatc aggccgtcga
     9661 cccgacgctc gacgaggaag tcgagatagg acgcctcgcg ctccggatcg tcgtccgcgt
     9721 tgcacagcag gatcgcgtag ccctcgccgc tcgcggcgtc ctcgacggcc ctgacgagct
     9781 gggggaagta ggggttctcg atgtcggtga cgatgaggcc gagcgtcagc gtcgagcggc
     9841 gcttgagcga gcgagcgacg tccgacggcc ggaagccgag ctcgcgggcc gccgcctcga
     9901 cgcgggcgtg ggtctcggga cgcgctcgtc cgatgcccga gaggacgcgg ctgaccgtgg
     9961 cggtcgacac accggcgcgc cgggcgacgt ccgcgatggt ggtcgccatc gccgcctagc
    10021 tgtgctggtt ctcgcccagg atcggggcgc tgtccgggtc cgtcccgagc gggaccgtgg
    10081 gaacgtcctc gccggcctcc tcgcggcggg cgagcatctc gagatcggcc tggagctcct
    10141 ccagctccga gccgccgccc atcaggccgt ggagctgttc gacggtcagc tccccgcggc
    10201 gatacgtccc gccggaccgg ccgcgggtca ggatcgtgaa cgaatccccg atcggcagag
    10261 cgtgctggac gttgtgggtg atgaagatca cgccgaggcc cttgttccgg gccgcgatcg
    10321 cgtggcgcag cacgaatgcc gattccttga cgccgagcgc tgacgtcggc tcgtcgagga
    10381 tgaggacgcg cgccccgaag tactcggccc gggcaatggc gagggtctgg cgctcgccgc
    10441 ccgacagcgt cccgacgagc tgctcggtgt cgcggacgtc gatcccgatg ttgtgcatct
    10501 cctcgcgggc gatcgcgtcg gccttggcgc tgtcgaagcg gcggaacggc ccgaagcctt
    10561 tcgtcggctc gttgccgagg aagaagttgc ggctgatgct catcaggggc atgaccgaca
    10621 ggtcctggta gacggtcgcg atccccgcgt cgagcgcggc ccgcggcgag gagaagatga
    10681 ccggctcgcc gtccaccgcg agcgttccgt cgtcgggcgg atggacgccc gagagggtct
    10741 tgatcagggt cgacttgccg gctccgttgt cgccgagcag gcagttgatc tcgccggccc
    10801 ggacggtcag cgacacgccc tcgagggcga cgaccgcgcc gaagtacttc gagatgttgc
    10861 gcgcctcgag gagcggcttg cgggccgctc gcgcggcggc ggccgtcgga gccggcccgg
    10921 cagcggaggg ggacatcgtc atggcgtgct ccttccgcgt cagcgcaggc cggcgaagcg
    10981 gcgccggatc cagttgttga ggacgacggc ggtcagcagc aggaggccga tcgcgatctt
    11041 gtaccagtcg gcgttgatcg aggcgtagac gatcccgatg ttggccatgc cgagcgcgag
    11101 tgcgccgagc gccgttccgg cgaccgaacc gtagccaccg gtcaggaggc agccgccgat
    11161 gactgcggtg gtgatcgcgg tcagctcgta gccggtcccg cgctggatgt ccgcggaggc
    11221 gaactgcatg ctctgacagg ccgccaggat cgaggccgag aaggcggtcg aggcgaacag
    11281 caggatcttg acccgggcaa cggggactcc gaggttgcga gcggcgacgg gtgagccgcc
    11341 gacgccggcg atccagttgc cgaacctgcg ccggcccagg acgtacgccc cgacgatcgc
    11401 gatcaggatc caccagacga ccgagacgta caggtccgat gtcgcgagat cgaacttgaa
    11461 cgacagcttc cagttgaaga actgggccag cggatcggcc gcgatcgcgg ccttgtcgat
    11521 cgagatgaac gtgatgttcg tcgcgctcag gacgaggacc gacgacagcc cggcgatgat
    11581 gaacagggtc gcgagggtga cgatgaacga cggcaacccg gtccgcacga cgatcaggcc
    11641 gttgaggacc ccgaggccgg tcgtcaggat catcgagatg atgatcgata gccagatcgg
    11701 caggccccag accgacgcgc agatgccgat gatgatcccc gagaagccga cgagggatcc
    11761 gaccgacagg tcgaactcgc cggcgatcat cagcagggag accgacgtcg cgatgatccc
    11821 gatctgggat gcgacgccga cgtagttcag cacgccgggg atggacagga acgcgttggt
    11881 gcccttcagg ccggccagca cggcgaacac gacgaagacg aacacgacga tgacgaacgc
    11941 cccggccgcc ggtttggcga ggaggcgttg gagcagagag acctgtcgga gccgttccgg
    12001 tgcgggcgtc ggcgccgcgt ccgccgtggt cgtcgccatc gaaatcctcc gcgaaggttg
    12061 gtggaggggc cgggccgagg cccgacccct cgcgcacgcg ccggcgaacc ggtgcggtgg
    12121 actcaggtca gcgcagaccc ttcgccgcga gggcgatgac ggcggctgcg ttgtccttgg
    12181 tgacgaagcc cgggccggtc atgatcaggc cggtgccgtc gccggtcggg aggttcttgg
    12241 tctctgcata gttcgtgagg atcacgatcg gcagatagcc ctgcaggaac tgctgctggt
    12301 cgatggcgaa gtccatcttc ccgtccttga tcgccgtcag cacgtcggcg ctgaggtcga
    12361 aggtcgcgag gtggatcggc ttgccggagg ccgtgacggc cagcagcgcc ggtccggcga
    12421 cggacgggcc gagggtcagg aggccgtcga tcgtcggatc cgactggatg gccgcggcga
    12481 tcgtcgcctg catcccggtc gggtcggaga tcttgccggt caggaccttg ccctggctgg
    12541 ggtcggtgag acccatgccc tggaagaggc cgttgcagcg cagcgtcagc gcctggttgc
    12601 cgacttcctg gttgaagcag acgacgttct tggcgcctgc cgccttgaac cgctcaccgg
    12661 cgccgatgcc cgcctggagc tcggtctggc cgacgtgggc caggatcccg aggcccttga
    12721 agacgtcgga gccggagttc atcgagacga ccgggatgcc cgcttcgacg gccttcttga
    12781 tcgccgggct gagggcgtcc gggttcggga tcgagacgac gaggccgctc ggcttcttgg
    12841 ccaccgcggc gtcgatcagg gacgacatgc ccggcatgtc gctctcctgg gccggtgcgt
    12901 tgtagttgac ggtgacgccc atgtcggcgg cgccggcctt gacgccgttg cgaacgacgc
    12961 cccagaagcc gtcgatcgcc tggccgtggg tcacgacctc gatcttgatg tcggcgcgct
    13021 tgaccagcgc cccatcgggg cccttggctc cggtggtggc gccggacgcg gccggggtcg
    13081 agttgcacgc gctgaggacg atcgcgatgc ccgcgagtcc cgtgaacagc gttcgcttgt
    13141 tcatccctgg tttcctcctc catggaacga tgtggtgggg tggatgctag gtcgttcgcg
    13201 gcgcccggat tcgtcgaatc cgcgcaaccg tcccgagcgt ccgccgcgat ctcaccgctc
    13261 gcccgagccg accgtggccc gaacgatcga cacctccttt caccgaccgg atccggtcga
    13321 tcagctgttg gcgatctcct cgacacgcac cggtcggtgc tcgtggagcg attgtgtggc
    13381 ggcctccgcg atccggaggg cctggacgcc gtcgtgggcc gtacacgggc tcggctcgtc
    13441 gccccgggcg acccggacga acgcggcgaa ctcggcgctg taggcggcag agaagcgatc
    13501 gaggaagtgc ggccaggcgg gcccggcctg gggcggcacg cccggctcga ctgagcggat
    13561 cggcgtccgc ggcccgagcc cgacactgat cgagtccttc gagccgaaga gctcggcccg
    13621 gatgtcgtag ccgagcgggt cgtggcgggc gacggtcagg acgccgagcg cgccgtcgtc
    13681 catccggagg gtcgcgacgg ccgtgtcgac gtcgttgtac ttggcgaaca tcgggaagcc
    13741 gcggacgtcg ccgtcggcat agacctcgac gacctcgtgg ccggtcagcc agcggatcac
    13801 gtcgaagtcg tggatcgaga agtcgcggaa caacccgccc gattgcggga tgtagccctc
    13861 gtgcggcggc tccggatcgt ggccgacgag gtggaagttg tagagcgttc cgagctcgcc
    13921 actctcgatc atccggtggg cctcgccgta gccggcatcg aaccgccgct ggaacccgag
    13981 ctggaacggg atgccgctct tctcgatgtg ccgggcgacc tcgatcgtcg cagtgatgtc
    14041 ctcggccagg ggcttttcgc agaacgtcgg ccggccgcga tccgccgatg cccggatcag
    14101 ctcggcgtgg gccgtggtcg ccgcggcgat gacgaccgcg tccgcctcgt cgagagccgc
    14161 ctccaggctc ggtgcggccc ggccgttgac ctccgcggcg gcttcagtgg cccgttccgg
    14221 cagcgcgtcg gcgatgacga gctcgtcgac cccgggtgtc gccgagagga ggcgggcgtg
    14281 caaccggccg atccggccgg cgccgagcag cgcgaccttc atcgtggagc tccttcggtg
    14341 gtggcggccg cgtcgcccgt cccggcgagc cggcgaagtg cagcggcgag cacccgccgg
    14401 ccgatcgccg cgctctcggc cggtggcggc cagccgctgt cctgctcgac catcagccag
    14461 cccgcgtagc ggcgctcggc aaggacgcca agcacgccgt cgaggtcgag cgcgccggcg
    14521 ccgagctcgg tgaacagccg ggcgcggatc gccccgccga agccgccggc ggtcccgtgg
    14581 cgaagcccgt cgagcacgtc ggggtcgacg tccttgaggt ggacgtgctc cacgcgctcg
    14641 ccgtaccggc gcagggcggt gaccgggtcg ccgccgccga cgagatagtg accgacgtcg
    14701 aggcagaagg ggaggaccgt cgggtcgatc gagcttgcca gtcgctcgac ctcctcgggg
    14761 gtctcgatgt acgtgccggc atgggggtgg aacgcgatcc gggcgccggc cgcccgggtc
    14821 tcggccgaga ccgtctcgag cagctcgatc gtccgggtcc agccctcgtc ggtcaggcgc
    14881 ggcgtcgcgg gatcggcggc gcggccggcg aacgggctcc ggtcggggga gccgtcgaag
    14941 gcgatgcaga ggacctcacc gccgccggcg cggagcaggc ggatgcgctc gcggacctcg
    15001 tcgagcgccg tcgcggtggg tccgtcgacc gtcgccggga tcgaggcgta gacctcggca
    15061 aggcgaaggt cccgggcggc gagggtggcc ttcaggtcgt cgccctcggg aaagccgagg
    15121 ccgagctggc agccgtcata ggcggtccgg gcgatgtcat cgaggatcgt ctcggcgctc
    15181 gtcccgagcc ggagctcggc gatgtcgacg ttgttccaga ggatcgggac ggtgccgatc
    15241 gaggcgccgc cgaggggacc tccggcggcg agacggcggc gcagcctggc cacctgttcg
    15301 acgacggcgg tcgatggcgc gacggtgatc attggtgctg ctcctcggct cgacccgcga
    15361 gccaccgagc gtgctgttcg gggtgatgcg gcgggctcgg tgcgtcccac cacccggtct
    15421 tgtcgggacc tgcgacggcg aggtcccggt cgaccttgac gtggacgagc gacgggccgc
    15481 ccgaggcgag tgcccggcgg accgccggct cgacctcgtc gggatgggtg gctgcttcgc
    15541 tgtggatgcc gaaggcggca ccgatggccg cgaaatccgg tgagtagatc gagccgtcgg
    15601 gcgtgacgaa gtcggtccag gcggtccgtc cgaagaagtt ctcctggccg cccttgatgc
    15661 tgatccagcc cgagttgtcg agcacgatcg tgcagaccgg ggtgccggcc aggaccgccg
    15721 cctggagctc ctgcatggtc tggaggaagt cgccgtcgcc gcagaccgcg acgacctcgc
    15781 ggtccggcag ggcgagctgg gcgccgatcg cggcgggcag ggtgaagccc atcgtcgaga
    15841 agccgccgga cgtgaggtgg gtccgcgggt ggcgggtcac ccagcgttgc ttgaccatgc
    15901 cctggggaag accggctccg gtcacgacga ccgcatcgtc gctcgtcgcc cgctggacct
    15961 cgcggaccgc ccgggccatc gtcatcggcg tggcccgcga cgcggacttg atctcgacct
    16021 gatcgaacca gtcggccttg agctgctgga tctcggcgaa gtaggcggtg gcgcgatagg
    16081 cggccggccc gccgcccggc ccgagcgccg cgaggaggtc gccgagggcc gccttggcgt
    16141 cgccgacgag ggcaacatcg accgggtagt tcttgccgat ctcgcgcggg tcgatgtcga
    16201 cctggatgag ccgcgacggc gggatggcga acgtcacgcc cttgcgatac gacgacgccg
    16261 accagtcggt gaagcgctgg ccgaccgaga ggacgacatc ggccgacgcg ctgaggaggt
    16321 tgccgcaggt cgaggccgtg tcgccgatcg tctgggcact gaggtcgtgg gtctcgtcga
    16381 tcgcgccctt tccgttccac gtcgtgacga ccggcgcgcc gagccgctcg gcgagggccc
    16441 ggacctcgtc ccaggcctcg gcgaggagca cgccgccgcc ggcgacgatc acgggccgca
    16501 gcgcgccgcg gagggacgcg gccgcgcgct cgacgtcgtc ggcagcgggt cggatccggc
    16561 cgcgcgcctc gcgctggtcg ggttccggca tccggacgtc ggccgcctcg gcctggatgt
    16621 ccatcggcag gtcgagcagg atcggtccgg ggcgcccgct cgtcatcgcg ttccatgccc
    16681 ggtggaggac gaacggcagg tcatcgaccc gcgacggctg ccaccattcc ttgacgaccg
    16741 gctcgaagac gcgtgggttg tcggcgtggt gccgtcgctc gaactcctgg aacagcccgt
    16801 gaccgcgcat gtacgtgtgg ggcgagccga tcgccagggc gacggcggtc gagtcgacat
    16861 aggccgtgcc catcccgacg acggtgttca tcgcacccgg tccgatgctc gtgaaggcca
    16921 gcatcggctg cccggtcgcg cggaagtagc cgtcggcgag gtggacggcc gactgctcgt
    16981 gcatgacctg gatcgtccgg atcacgtcct gccggtcgag aagggcgtcg gtgatcgccc
    17041 agcagccgtg accggggatg ccggccgcga acgggacgcg ctgacggacg aggtactcga
    17101 cgaggatctg ggcgccggtc agccggctga gcgcggtgac ggagtcgctc aacggtgctc
    17161 caagcgttac gatgtaatcg tttacaagga tgcggacgga gcgtacaaac ccggttgccc
    17221 ggtgtcaacg gccgatgcgc cctgattgtc gccgggttgc ggagccggcc caggcggagt
    17281 cgggcctcga ccgtgcgttc gccccgtccg ggccccgcac tggtccgcct ccgggcttgg
    17341 cggtaaggtg tcaatgactg gcgtccgtgt cgcgggcgcg ctgaaacgga agggtcgagc
    17401 aggatgacgc aggtcgccga gccgggcagt gcccgggccc aggacgaggc gaacggaacc
    17461 gccaccggcg cggttcacca ctggatcggc ggccggatcg tcgaggggac gtccggccgg
    17521 cgtggcccgg tctacgaccc ggccaccggt cgcatcgcgc gcgaggtcga cttcgcttcg
    17581 gtcgacgagg ttgccgcggc ggtcgccgcc gcgaaggccg ccttccccgc ctggcgcgcg
    17641 acgtcgctgt cgaagaagac cgacatcctg ttcaagatcc gcaacctcgt cgaggcgcac
    17701 cgcaaggagc tcgccgccca cctgacggcc gagcacggca aggtcccgtc ggacgccctc
    17761 ggggagatcg cccgcggcat cgagaacctc gagttcgcct gcggcatccc gaacctgctc
    17821 aagggcgggt tcagcgagca ggtgtccggc ggcgtcgacg tcttccagat ccaccagccg
    17881 ctcggcgtgg tcgccgggat cacgccgttc aatttcccgg ccatggtccc gatgtggatg
    17941 ttcgcgacgg cgatcgcgac cggcaacacg ttcatcctca agccgtccga gaaggacccc
    18001 tcggcggcga acttcatggc cgatctcctc cacgaggccg gcgtccccga cggcgtgttc
    18061 aacgtcgtcc atggcgacaa ggtcgcggtc gacgcgctcc tcgagcaccc cgacatcgcg
    18121 gcgatcagct tcgtcggctc gaccccgatc gcccgctaca tctacgagac gggaacgaag
    18181 aacggcaagc gcgtgcaggc actcggcgga gcgaagaacc acatgatcgt cctgcccgat
    18241 gccgacatcg acatggcggc cgacgccgcg atctcggccg gctacggctc ggccggtgag
    18301 cgctgcatgg cgattgccac gatcgtcgcc gtcggcgatg tcgccgaccc gctcgtggag
    18361 gcgatcacga agcggctgcc gaaggtcaag gtcggaccgg gcagcgatcc cgaggccgag
    18421 atgggcccgc tcgtgacccg ccagcatcgc gacaaggtcg cctcgtacct cgacagctcc
    18481 gcggcccagg gcgcgaccgt cgtcgccgac ggtcgcgagc acccgctgtt caaggactcc
    18541 gacgggttct tcctcggcgt ctcgctcatc gactacgtct cgaccgagat ggacgcctac
    18601 aaggacgaga tcttcggccc ggtcctgacg atcgtccggg tcccgaccta cgaggaagcg
    18661 gtccgcctga tcaacgacaa cccgtacggc aacgggaccg cgatcttcac ccgcgacggc
    18721 ggcgccgccc gccagttcca gttcgaggtc aatgccggga tggtcggaat caatgtcccg
    18781 atcccggtgc cggtcgccta ttacagcttc ggcggctgga agagctcgct cttcggcgac
    18841 ctccacatgt acggtcccga ggggatccag ttctacaccc gggccaagat cgtgacccag
    18901 cgctggccgg accccggcac gtcgaagatc gatctcgggt tcccgcggac acgctgatgg
    18961 cggcgctcga ccccgcgcca ggctcagcga ccggccggaa cggccaggtg ttcatcgtca
    19021 gcgctgccag gacgccgatc ggcaagttcg gcggcgtgtt cgccgacacg ccggcgacga
    19081 ccctcggcgg cgtcgcgatc cgggccgcga tggagcggtc cggcctgccc ctcgacaccc
    19141 cggtcgacga ggtcctgatg ggccaggtgc tccaggccgg cgtcggccag gcgccggccc
    19201 gccaggcggc gctctcggcc gggctcgccg ataccacctc ggcgacgacg atcaaccggg
    19261 tctgcggctc ggggctgaag gcgatcatgc tggccgccgc cgagatccgc gccggcgacg
    19321 cggagatcgt cgtcgcgggc ggcatggaga acatgaacct cgcgcccttc ctcctgccga
    19381 aggcccggtt cgggtaccgg ctcgggaacg cgacgctggt cgacgccgcg gtcgccgacg
    19441 gcctgacctg cgtgttcgag ggctgccaca tgggcaccca cgccgagcgg gtcgcgatcc
    19501 acgaccacat cagtcgcgag gaccaggacg cgttcgcgct cgcctcgcag cagcgggcga
    19561 tcgcggccca ggacgccggg cggtttgctg cggagatggc gccggtgacg atccacgacg
    19621 cgaagggccg cgagacggtg gtcgaggtcg acgagggccc ccggcgcgac tcgagcctgg
    19681 aggcgctggc ccggctgaag ccggtcttcg acctgccgac cggcgaggac cgcggggatg
    19741 cctcggtcgg cacggtcacg gccggtaacg cgcccgggat cacggacggc gccgccgcga
    19801 cgatcgtcgt gagcgagcgg gccgtggatc gtttcgggtt gacgccgctc gcccggatcg
    19861 tcggctacac ccaggccgag atcgccccga aatggctgtt cctcgccccg atcaccgggg
    19921 tccgccgcct gctcgaccgg atcgagctgc cgatcgaggc gttcgacctg atcgagatca
    19981 acgaggcgtt cgccgcccag acgctcgccg atgggcggga gctcgggttc gactgggaca
    20041 aggtcaacgt caacggcggg gcgatcgccc tcggccatcc gatcggcgcg tcaggggcca
    20101 ggatcgtcgc gacgctcctg cacgagctcg cgcggcgcga gggccgctac gggctggcca
    20161 cgttgtgcct cggcgggggt gggtcggtgg cgatggccct cgaacgagtg gtcgcctgac
    20221 cgatctgctg gagaggagca ccgaagcgtg acactcatcg acagcacggc gccagcgctg
    20281 gccgacacgc cgctcggccg aacaatcgcc accaccgcca ccgacatctg gaacgattcg
    20341 tgcgcgatcg acgagctcga gtacgcgatc tcgttcgggg cggtcggcgc gaccgccaac
    20401 ccgacgatcg tgaccgacgt ctggaagcgc gagccggcgc gctggaaggc gcgcgtcgcg
    20461 gccctggcgg tcgagcgccc ggacgcgagc gaggtcgatc tcgcctggca ggtcgtcgac
    20521 gagatgtcca cccgggcggc gccgctgctc ctgccggcgt tcgaggccca cggcgggcgc
    20581 cagggccggc tgtccaccca gacggacccg acgttctacc gctccgctga gcggatgctc
    20641 gcccactcgc tccatttcga cagcctcgcc ccgaacatca tcgtgaagtt ccctgccacg
    20701 tccgccggga tcgacgcgat ggaggaggcg agcgcgcgcg gcgtcagcgt caacacgacc
    20761 gtctgcttct cggtcgccca ggcgctcgcc gccgcggagg cgatcgagcg cggcatgcga
    20821 cgtcgcgagg cggccggcga gtcgaccgcg gcgatgggcc cggtcgtcac gatcatggtc
    20881 ggccgggtcg aggactggct ccgggtccag accgaacgcg acgggatcat cgccgatccg
    20941 gccgcgctgc catggtccgg cgtggcggtg ttcaagcggg cctacgggct gttccgtgag
    21001 cgcggctacc ggccgcgcct gctcgcggcc gcgatccgcc accactatca ctggtcggag
    21061 ttcatcggcg gcgacatcgt catcacgctg cccgcggcct ggcagaagcg gttcaatgcg
    21121 tcctcgatcg aggtccggcc gcggatggac gatccggtcg atccgcggat cgtcgccgag
    21181 ctggcgggca ggtatcccga cttcgtccgg gcctacgagc ccgacggcct ccggcgcgac
    21241 gagttcgacg cgttcccccc gacggcccgc acgctccgcg cgttcatcgc gtcatatcac
    21301 gagctgctcc accaggtgac cgacgcgatg atcccgaacc ccgacctccg gccggcctcc
    21361 tgaccccgat gcccgagccg accatccaca cgcgcgccct gcccagcgag ctcgcccccg
    21421 ccgaccttcg gcttcgacca gatccggacg gcgccgtctc gctcgacccg acccgcgccg
    21481 gctggcgcta tctctcgttc gccgtggccg cggtcccgcc aggcggcctg aggatcccgg
    21541 ccgcgcagga catggagacc tgtgtggtcg tccagcgcgg tcacggcacc acgatccggg
    21601 cgggcgatcg gcactggtcg ctgccgggtc gcgaggacgt cttcgccgac ctgccatcgg
    21661 ccctgtggct gcccgacggc cgtgccctga ccgtggaggt ggaacggcgg cctgcggtgg
    21721 ggcccgccct cgtcgcggtg gcgcgggcgc cacgttccgg acgtgacggc gtcgccgcgg
    21781 gtctcgacca cgacatcgtc gggcacgatc gcgatgggct ccgcggcgcc ggcaacgcga
    21841 cccgccagat cagccacatc atcgcgccca cgttcccggc cgaccgtctc gaggtggtcg
    21901 aggtctacac gccgtcgggc aactggtcga gctggccacc gcacaagcac gacgtcgatg
    21961 acatgcccgg cgaggcggtt ctcgaggaga tctatcacta ccagttccgc cgacccgagg
    22021 cctgggcgat ccagcgggtc taccggctcg accgctcgcg cgacgcattg ttcgaggttc
    22081 gccacgggga cgccgtcatc gtgaccgacg gctatcatcc cttcagcgcg acgcacggcg
    22141 acgacgccta ctacctcaac gccctcgccg gcgaccggcg gacgatggcc tgctcgttcg
    22201 atcccgatct cgattgggtc cgcgcgacct gggccgggat ggagctcgat ccacgcgtgc
    22261 ccgtggtgcc gcctcgtcga gcgggctgaa ccggggcgac gatcgcggc
//
LOCUS       DATAIW010000518         5462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_146580, whole genome shotgun sequence.
ACCESSION   DATAIW010000518 DATAIW010000000
VERSION     DATAIW010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146580"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1334)
                     /locus_tag="VHM48_15245"
     CDS             complement(51..1334)
                     /locus_tag="VHM48_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2756825.1"
                     /translation="MDERSDPDRDVAPPSAISRRDVLRGAALGGVAVFVAACGSSTTP
                     APSTSPAGLATASPPPSPEPTAPSAPPTVGPSSAATTGPTVGLGHKIAGLMIVGFRGS
                     ELADAPWVRTALVESGLGGVILFDRDQLTGADRNVLSPAQVKRLTGDLRAAAPDRDII
                     VSVDQEGGIVTRLGPGHSFPAVASEATIGRGTAGAARTWARAIASTLAANGFNLNFAP
                     VVDLDVNPDSPAIGALDRSFSADPDVVVARATIAIDEHRAAGVRTTLKHFPGIGSSTT
                     NTDFGVADVTKTWTATELEPFAQLVAAGKADLVMAGHVVNGQFDADHPASLSPTIVTH
                     VLRGQLGWDGIVVTDDLQAAAITTAFGRDEAILLAIEAGNDLLLFANQQVYDPKLVSR
                     VVKLVAAAVASGRIPRSRIDEAYDRIQALFGGTTS"
     gene            1456..1809
                     /locus_tag="VHM48_15250"
     CDS             1456..1809
                     /locus_tag="VHM48_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756826.1"
                     /translation="MRNRLLASALLALALTGLTGGAALAHPDNEQTLHFVLTCDDGHV
                     WNASFNGGPSAFHLDGGQLFIWKQIAYVTPDGQSGTIGHGIQGFAGAPTVICTYTGAV
                     SGNAYTVTGFYPPAG"
     gene            1939..2892
                     /locus_tag="VHM48_15255"
     CDS             1939..2892
                     /locus_tag="VHM48_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2756827.1"
                     /translation="MNPMIQVEHLVKRYKKAVGPAVDDISFMVGAGELFAFLGPNGAG
                     KTTTISILTTTLAKTSGIVRIDGHDLDRDATAVRRSIGIIFQNPSVDLHLSGEENIRL
                     HVALYGLHAYRPFYRTMPADYRARIEELSDVVGLGAEVFRPIKTYSGGMKRKLEIIRS
                     LMHRPGVLFLDEPTSGLDPVSRHSLWQYLRRVRAADGTTIFLTTHYLEEAEEADRVCV
                     IDHGRIATMGTPDELKAELLERSIVLDAADRPALLAELRSLGISADLDELGLVRVAYE
                     GPTAQHLIARLRTPLTVLRVHDPSLEDAYVELLRSAEETAA"
     gene            2889..3725
                     /locus_tag="VHM48_15260"
     CDS             2889..3725
                     /locus_tag="VHM48_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756828.1"
                     /translation="MTARAAAPPNIRSGVSGRLAREANATFAIAWREVLRAIKSPSSL
                     AFTVIFPVIFMGVLGGSISQNLGGALPYAYLPFMLIGMVANTLYQGTIVGVTNLVEER
                     ENDFTAELFVAPVSRYAVLLGKIVGSAIASLVSLVGVVAMIFVMQIPMDVGDLLRVIA
                     LAPILALAGGSLGVFFIGFVQDPKTAAAGVALLVFPQIFLSGALIPTAASTGILGILA
                     KLMPMTYSIDLARNIFYAGKPEAVYTVLNSPWLDLAVTVGFFLVFTIVGTYMFVRADR
                     NR"
     gene            complement(4209..4700)
                     /locus_tag="VHM48_15265"
     CDS             complement(4209..4700)
                     /locus_tag="VHM48_15265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2756829.1"
                     /translation="MTSVTITTEKGDIDVDLFTTDAPKATKNFVDLARKGFYDDVIFH
                     RVIPGFVAQAGDGEHGKKASLNAGRVGTGGPGYKFEDEPIKGNYVRGALAMANAGPNT
                     NGSQFFICHQDLTGKLPKNYTLFGQVTKGMDVVDAIVSAPRNSKDLPDEPVAITGITI
                     QED"
     gene            complement(4732..>5462)
                     /locus_tag="VHM48_15270"
     CDS             complement(4732..>5462)
                     /locus_tag="VHM48_15270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2756830.1"
                     /translation="PLAARGDIDVLVAGGVIKFGSASPSSAINGQVSLAPGGSPAPGS
                     SVAPVGARGTNCPTAEPPAATAGQTRVVTIETAKGSIAITVDPSLGPKAAGNFIALAS
                     CGFYDGLVFHRLVPGFVIQGGDPAGTGAGGPGYEFADDPVNVPYARGVVAMANSGPDT
                     NGSQFFIVLDDNTGLKPNYSVFGRVTAGMDVVDAIASMPNAGGQTNAALDPVAMTKLT
                     VGPPVAAIPSAAGSSTPASSPAPS"
BASE COUNT          800 a         1924 c         1865 g          873 t
ORIGIN      
        1 cacctcgttg gcgatgatct ggctcgccac gcggcagccc tgaacgtttg tcagctggtc
       61 gttccgccga acaacgcctg gatgcggtcg taggcctcgt cgatccgcga acgcgggatc
      121 cggccggagg cgaccgccgc ggcaacgagc ttgacgacgc gcgagacgag cttcgggtcg
      181 tagacctgct ggttggcgaa caggaggagg tcgttgccgg cctcgatggc gaggaggatc
      241 gcctcgtcgc gcccgaacgc cgtcgtgatc gccgccgcct ggaggtcgtc ggtgacgacg
      301 atcccgtccc agccgagctg gccgcggagg acgtgggtga cgatcgtcgg cgacagggac
      361 gccgggtggt cggcgtcgaa ctggccgttg accacgtggc cggccatgac gaggtcggcc
      421 ttgccggcgg cgacgagctg ggcgaacggc tcgagctcgg tcgcggtcca cgtcttcgtc
      481 acgtcggcga cgccgaagtc ggtgttcgtc gtcgagctgc cgatccccgg gaaatgcttg
      541 agcgtcgtcc ggacgcccgc cgcccggtgc tcgtcgatcg cgatcgtcgc ccgcgcgacg
      601 acgacgtccg ggtcggccga gaacgaccgg tcgagcgcgc cgatcgcggg gctgtcgggg
      661 ttcacgtcga ggtcgacgac cggggcgaag ttcaggttga agccattggc tgccagcgtg
      721 gacgcgatcg cgcgcgccca cgtccgcgcc gcgccggcgg tacccctgcc gatcgtcgcc
      781 tccgacgcga cggcgggaaa gctgtggccc ggcccgaggc gggtcacgat cccgccctcc
      841 tggtcgacgc tgacgatgat gtcgcggtcg ggcgcggctg cgcgcaggtc gccggtcagg
      901 cgcttgacct gggccggtga caggacgttc cgatcggcgc ccgtcagctg gtcgcgatcg
      961 aacaggatca cgccaccgag ccccgattcg accagcgcgg tccggaccca cggggcgtcc
     1021 gccagctcgc tcccgcggaa cccgacgatc atcaggccgg cgatcttgtg gccgagaccg
     1081 acggtcggcc cggttgtcgc cgccgaggac gggccgaccg tcggcggcgc ggagggagcg
     1141 gtcggctccg ggctcggcgg cgggctcgcg gtcgccagcc cggccggcga ggtcgacggg
     1201 gcgggcgtcg tcgaggaccc gcatgcggca acgaacacgg cgacgccgcc gagcgccgca
     1261 ccgcgcagga cgtcgcgccg cgagatcgcc gacggcggcg cgacgtcccg atccggatcc
     1321 gagcgttcat ccatcgcccg aggctaccgc ggctcggcgt tggtcgtccg cggcgcggta
     1381 ggcccgatcg tgggacgatc cacggaccgc agccggcatc cgggggccag gtcgccgacc
     1441 gatggaggaa gccccatgcg caaccgcctt ctcgcttctg ccctgctcgc cctcgccctg
     1501 accggcctga ccggtggcgc ggcgctcgct cacccggaca atgagcagac gctccacttc
     1561 gtcctgacct gcgacgacgg gcacgtctgg aacgcatcgt tcaacggcgg cccgtcggca
     1621 ttccacctcg acggcggcca gctgttcatc tggaagcaga tcgcctacgt cacgccggac
     1681 ggccagagcg ggacgatcgg ccacggcatc caggggttcg ccggcgcgcc gaccgtgatc
     1741 tgcacctata cgggcgcagt cagcggcaat gcctacacgg tgaccggctt ctacccgccc
     1801 gccggctgac cgcgagccga gggcccgccc cgaccggatc gccatccggc ctccacgcct
     1861 gctccacaat cgcccgcgag gatcgcatca ggcccgggct cgacccggca cgacgacgaa
     1921 tcctcgcggg agcagccgat gaacccgatg atccaggtgg agcacctggt caagcgctac
     1981 aagaaggcgg tcggcccggc cgtcgatgac atcagcttca tggtcggggc gggcgagctg
     2041 ttcgccttcc tcggcccgaa cggcgcgggc aagaccacga cgatctcgat cctgacgacg
     2101 acccttgcca agacgagtgg catcgtccgg atcgacggcc acgatctcga ccgcgacgcg
     2161 accgcggtca gacggagcat cgggatcatc ttccagaacc cgagcgtcga tctccacctg
     2221 tcgggcgagg agaacatccg gctccacgtc gcgctctacg gcctgcacgc ctaccgcccg
     2281 ttctaccgga cgatgccggc cgactaccgg gcccggatcg aggagctgtc cgacgtggtc
     2341 gggctcggcg ccgaggtctt ccggccgatc aagacgtatt cgggcgggat gaagcggaag
     2401 ctcgagatca ttcgctcgct catgcatcgg cccggggtgc tctttctcga cgagccgacg
     2461 tccggcctcg acccggtcag ccgtcactcg ttgtggcaat acctccgccg cgtccgggcg
     2521 gccgacggga cgacgatctt cctcacgacc cactacctcg aggaggccga ggaggccgac
     2581 cgggtgtgcg tcatcgacca cggccggatc gccacgatgg gaacgccgga cgagctcaag
     2641 gcggagctgc tcgagcgctc gatcgtcctc gacgcggcgg atcggccggc ccttctcgcc
     2701 gagcttcgca gcctcggaat ctccgccgac ctcgatgagc tcgggctcgt gcgcgtcgcg
     2761 tacgagggcc cgaccgcgca gcacctgatc gcccgcctgc ggacgccgct gaccgtcctc
     2821 cgcgttcacg atccgagcct cgaggacgcc tacgtcgagc tccttcgctc ggccgaggag
     2881 accgccgcat gaccgcccgc gccgccgccc caccaaacat ccgaagcggc gtcagcggtc
     2941 gcctcgcccg ggaggcgaac gcgacgttcg ccatcgcctg gcgcgaggtc ctgcgggcga
     3001 tcaagagtcc gtcatcgctc gcgttcaccg tgatcttccc ggtgatcttc atgggcgtcc
     3061 tcggcggcag catctcgcag aacctcggcg gggcgctgcc gtacgcctat ctcccgttca
     3121 tgctcatcgg gatggtcgcg aacaccctct accagggcac catcgtcggc gtcaccaacc
     3181 tcgtcgagga gcgcgagaac gacttcacgg ccgagctctt cgtggcgccg gtctcgcgct
     3241 acgcggtcct gctgggcaag atcgtcggct cggcgatcgc ctcgctcgtg tcgctcgtcg
     3301 gggtcgtcgc gatgatcttc gtcatgcaga tcccgatgga cgtcggcgac ctgctgcggg
     3361 tgatcgcgct ggccccgatc ctggccctcg ccggcggctc gctcggggtg ttcttcatcg
     3421 gcttcgtcca ggacccgaaa accgcggccg ccggcgtcgc cctcctcgtc ttcccccaga
     3481 tcttcctgtc cggggccttg atccccacgg ccgcctcgac gggcatcctc ggcatcctcg
     3541 ccaagctgat gccgatgacc tacagcatcg atctcgcccg gaacatcttc tacgccggca
     3601 agccggaggc cgtgtacacc gtgctcaaca gcccgtggct cgatctcgcg gtgaccgtcg
     3661 ggttcttcct cgtgttcacg atcgtcggga cgtacatgtt cgtccgggcc gaccgcaacc
     3721 gctgaatctc ctgacgcggg gcttgatcgc tccgggtgat cagattccgc acaaacgagc
     3781 gtggtccgac tgcagatctg ccatctcgtg gcccgatctg cccgtggccg cgctcgttcg
     3841 tgctctgatc gatcagtcgg gacgatcaga cccggacccc tctcgcggag atagttcgag
     3901 gccggctccc gaaggggccg gcctcgatgc tgtcccggtg aaagtccggc gaaccggaca
     3961 cccgccacgc gccaagggca tctatcacgt cggctgcacc gactaccctg accctaccta
     4021 cccatgacct tggttctggg gcagcttggc tgacaagcag cgcagtcgcc atgctactcg
     4081 ttgcccctcc gaaagccaag agttttgatg cacggcgcga tgtaaattcc ttaacgttcg
     4141 ttgcgtgcgc atatcgggcg gctcggcatc cggccgtccg ggagcctccg ccgagcgtga
     4201 atcgcggctc agtcctcttg aattgtgatg ccggtgatcg ccaccggctc gtcgggcagg
     4261 tccttcgaat tgcgcggcgc ggacacgatc gcgtcgacca cgtccatgcc cttggtgacc
     4321 tggccgaaca gcgtgtaatt cttcggcagc ttgccggtca ggtcctggtg gcagatgaag
     4381 aactggctgc cgttggtgtt cgggccggcg ttggccatcg ccagggcgcc ccggacgtag
     4441 ttgcccttga tcggctcgtc ctcgaacttg tagccgggcc cgccggtccc gacccgaccc
     4501 gcgttcaggc ttgccttctt gccgtgctcg ccatcgcccg cctgggcgac gaagcccggg
     4561 atgacgcggt ggaagatcac gtcgtcgtag aagcccttgc gggcgaggtc gacgaagttc
     4621 ttggtcgcct tcggcgcgtc ggtcgtgaac aggtcgacgt cgatgtcgcc cttctcggtg
     4681 gtgatcgtga cgctggtcat gggttcctcc tgtgggatgg gcaaggacgg ctcacgacgg
     4741 agccggggac gaggcagggg tggacgatcc cgcggccgat ggaatggccg cgaccggcgg
     4801 gccgaccgtc aacttcgtca tggcgacggg atcgagggca gcgttggtct gcccgcctgc
     4861 attcggcatc gacgcgatgg catcgacgac gtccatcccg gcggtcacgc gcccgaatac
     4921 cgagtagttc ggcttcagcc cggtgttgtc gtcgaggacg atgaagaact gcgacccgtt
     4981 cgtgtccggg cccgagttgg ccatcgccac gaccccgcgg gcgtacggca cgttcaccgg
     5041 atcgtcggcg aactcgtagc cgggcccgcc ggcgcccgtg ccggcggggt cgccgccctg
     5101 gatcacgaac ccgggcacga gtcggtggaa caccagcccg tcatagaagc cgcaggacgc
     5161 gagggcgatg aagttgccgg ctgccttcgg gccgagcgaa ggatcgaccg tgatcgcgat
     5221 cgagcccttg gcagtctcga tcgtgacgac gcgggtctgg ccggcggtcg cggccggtgg
     5281 ctcggccgtc gggcagttcg tgccgcgggc gccgacgggt gcgacggacg agccgggcgc
     5341 cggcgacccg ccgggcgcga gggacacctg gccgttgatc gcactgctcg gactggcgct
     5401 gccgaacttg atcacgccgc cggcgacgag gacatcgatg tcgccacggg cggccagcgg
     5461 gc
//
LOCUS       DATAIW010000519         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_265879, whole genome shotgun sequence.
ACCESSION   DATAIW010000519 DATAIW010000000
VERSION     DATAIW010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265879"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VHM48_15275"
     CDS             complement(<1..601)
                     /locus_tag="VHM48_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756831.1"
                     /translation="MPLNFLRRKKAQGAAVTPAASTNGARATRTGVAFDGVTEEWRLV
                     GRMNVEGRLSDALNKRESLAIHDVRWAPIDGSAPLVEAPGLKSVDPYDLILVLAGEGS
                     MPELTDSERAAHRVHKVSYDVALEAPPFRIMGTVFMHPGYEPDRLLDRATEMFVPVVD
                     AVAMFGDQRVGDPDVETILVNRFYLRGVEQIDKRTGLKPQ"
     gene            complement(621..>2055)
                     /gene="acs"
                     /locus_tag="VHM48_15280"
     CDS             complement(621..>2055)
                     /gene="acs"
                     /locus_tag="VHM48_15280"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2756832.1"
                     /translation="GRINDAEAKLVITADGGWRRGKPAELKPAVDEAVQSTPTVEHVL
                     VVRRLGDQAPGCVMADGRDVWWHEVVERQSENCPPVPLDSEHMLYLLYTSGSTAKPKG
                     ITHTTAGYLVGTSYSHRQIFDIKPDDVYWCAADVGWVTGHSYIVYGPLANATTGVMYE
                     GTPDTPAWDRWWRIIEEYKVSILYCAPTAIRAFMKQGAQHPAGHDLSSLRVLGSVGEP
                     INPEAWLWYREHIGGGTAPIVDTWWQTETGQILITPLPGVTTTKPGSATFPFPGIEAD
                     VVDGQGNSVPFGGGGYLVLKRPWPAMLRGIYGDPERYKSTYWSRFPGMYFAGDGAKRD
                     EDGYFWLLGRVDDVMNVAGHRLSTTEIESALVSHPAVAEAAVVGGPDAVTGEAIISFV
                     ILRLGFEPTPELGNELRAHVATKISPIAKPKSLMFVPDLPKTRSGKIMRRLLRDIAQG
                     RTLGDTTTLADAGVVETIRANTGAAEE"
BASE COUNT          299 a          704 c          719 g          333 t
ORIGIN      
        1 tctggggctt gaggccggtc cgcttgtcga tctgctcgac gccccggagg tagaagcggt
       61 tcacgaggat ggtctcgaca tcggggtcgc cgacccgctg atcgccgaac atggccaccg
      121 catcgacgac cggcacgaac atctcggtcg cccggtcgag gaggcggtcc ggctcgtatc
      181 caggatgcat gaacaccgtg cccatgattc ggaacggggg ggcctcgagg gcgacgtcgt
      241 aggacacctt gtggacgcgg tgggcggccc gctcggaatc ggtcagctcc ggcatcgaac
      301 cctcgccggc aaggacgagg atcaggtcgt acggatcgac gctcttgagc ccgggcgcct
      361 cgaccagcgg cgccgagccg tcgatcgggg cccagcggac gtcatggatc gcgagcgact
      421 cccgcttgtt gagcgcgtcg gacaggcggc cctcgacgtt catccggccg accagccgcc
      481 attcctcggt cacgccgtcg aacgccacgc cggtgcgcgt ggcccgcgcc ccgttcgtcg
      541 aggccgccgg ggtcacagcg gcgccctggg ccttcttgcg ccggaggaag ttcagtggca
      601 cgccgctccc ctcgcctcga tcattcttct gcggctccgg tgttcgcgcg gatcgtctcg
      661 acgaccccgg cgtcggcgag ggtcgtcgtg tcgccgaggg tccggccctg ggcgatatcg
      721 cggagcagcc gccgcatgat cttgcccgat cgagtcttcg gcaggtcggg cacgaacatg
      781 aggctcttgg gcttggcgat cgggctgatc ttcgtcgcga cgtgggcccg cagctcgttg
      841 ccgagctccg gggtcggctc gaacccgagg cgaaggatca cgaacgagat gatcgcctcg
      901 ccggtcaccg catcgggtcc gccgacgacg gctgcctcgg cgaccgccgg atgggacacg
      961 agggcgctct cgatctcggt cgtcgacaga cggtggccgg ccacgttcat cacatcgtcg
     1021 acccggccga gcagccagaa gtagccgtcc tcgtcgcgtt ttgcgccatc gccggcgaag
     1081 tacatcccgg ggaaccggct ccagtacgtc gacttgtagc gttccggatc gccgtagatc
     1141 ccgcgcagca tcgccggcca cggccgcttc aggacgaggt agccaccacc gccgaacggc
     1201 acggaattgc cctggccgtc gaccacgtcg gcctcgatcc cggggaacgg gaacgtcgcg
     1261 ctgccgggct tggtcgtcgt gacaccgggc agcggcgtga tgaggatctg gcccgtctcg
     1321 gtctgccacc acgtgtcgac gatcggcgcg gtgccgccgc cgatgtgctc gcggtaccag
     1381 agccaggcct cggggttgat cggctcgccg accgaaccga ggacccgcag cgacgagaga
     1441 tcgtggccgg ccgggtgctg ggcgccctgc ttcatgaagg cccggatcgc ggtcggggcg
     1501 cagtagagga tcgagacctt gtactcctcg atgatccgcc accagcggtc ccatgccggc
     1561 gtgtccggcg tgccctcgta catgaccccg gtcgtcgcgt tggcaagcgg gccgtagacg
     1621 atgtagctgt ggccggtcac ccagcccacg tcggccgcgc accagtacac gtcatcgggc
     1681 ttgatatcga agatctggcg atggctgtac gacgtcccga cgagatagcc cgccgtcgtg
     1741 tgggtgatcc ccttcggctt ggcggtcgag cccgacgtat agagcagata gagcatgtgc
     1801 tcgctgtcga gggggaccgg cgggcagttc tccgactggc gctcgacgac ctcgtgccac
     1861 cagacatctc ggccgtcggc catgacgcag ccgggcgcct ggtcgccgag acggcggacg
     1921 acgaggacgt gctcgaccgt cggcgtcgac tggacggcct catcgaccgc gggcttgagc
     1981 tcggccggct tgccgcgccg ccagccgccg tcggcggtga tgacgagctt cgcctcggcg
     2041 tcgttgatcc ggccc
//
LOCUS       DATAIW010000520         6694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_266863, whole genome shotgun sequence.
ACCESSION   DATAIW010000520 DATAIW010000000
VERSION     DATAIW010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266863"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VHM48_15285"
     CDS             complement(<1..857)
                     /locus_tag="VHM48_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756833.1"
                     /translation="MQAIDVRDREIERRLEAFARARLSPDPQSVARARARVMREARLQ
                     IDASRIAAYIAPSVALIPRRSLTRRMAMPLLAASVWLGIAVGSISAAQAGGPLYATRM
                     WVENATLPADSASRASAELGRLESRLAEAYSASVRGDARAVQAALDAYREIADQTIAA
                     ATGDQALEALVAAALDKHRVVLTAVAAGLEGKGNDTAAAAVEASIQRAIDHNQVVIDR
                     LEATGAGAGNGSTGGTVSNPGATGGTGTGTGTGTGTGTGTGTGTGTGTGAYNGDGSGA
                     GSTGSGSAG"
     gene            complement(862..1470)
                     /locus_tag="VHM48_15290"
     CDS             complement(862..1470)
                     /locus_tag="VHM48_15290"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2756834.1"
                     /translation="MTIDDAAIERLVTEAQQGDAWAFGLIFDQYHEPIYRFIASRVHR
                     PSDAEDLTQHVFVKALEALPRYESRGIPFGGWLFRLARNAVIDHVRTRHDHAELDSII
                     EWTHGDAGPDEIAVVRQELDAVGVALAGLTGEQREAIALRFFAGLSAREAAEAMGKQE
                     GTVRGLQFRAIAALRRQLGIELDGSDVAEMRAPRRANATTDG"
     gene            complement(1493..2401)
                     /locus_tag="VHM48_15295"
     CDS             complement(1493..2401)
                     /locus_tag="VHM48_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX2756835.1"
                     /translation="MNADVAGSHLPDPTVQARDLPLDAHVHTDLSPDSDVPIDAYAAE
                     AIERGITEIAITDHVDFVPGTPAFSFATFDQRERVVREAAERWAPRGVAIRFGVEITY
                     DRAHEPDIRDHLARHVYDYVIGSVHVYTDSPFHETHVAAWTAGRPLAEIVAPYFDEVL
                     DAIRSGLFDTIGHLDVVKRYLAPHVTPAQLAAAPELYEPLLVALVETGVALEVNTSGL
                     RQGAGETYPPAPIVERYQLLGGTRVVIGSDAHRNRSFTAGLEAGYGVVAAAGFGQLAF
                     RRGGSRVGVTLPDRFRTRVGVEGANT"
     gene            complement(2444..3670)
                     /locus_tag="VHM48_15300"
     CDS             complement(2444..3670)
                     /locus_tag="VHM48_15300"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX2756836.1"
                     /translation="MSTDLRPDPLAFLGEELADLRDRHLYRPLRVMSSAQGPIVSVDE
                     HRLISLSSNDYLGLTHHPRLRKAALDAVRDFGAGSGAVRTIAGTMTLHEDLERELAEF
                     KGTAATLTFQSGFSANTGVIPTITGEQDLIVSDELNHASIIDGMRLSKAPRKIYKHAD
                     TDSLRAVLDEAVEKGREGTGLPYRLILVVTDGVFSMDGDIAPLPGIVEAAESANAAVF
                     VDDAHASGVLGRDGRGSVDHFGLHGRVAIQVGTLSKAVGVLGGYVAGSQDLRDILVQR
                     ARPFLFSTSHPPAVAAACREAIKVMQEEPELLERLWANTKRFKAELARLGFDTGRSET
                     PITPVMMGDPDTAGRFSQRLFEEGVFAQPVVFPTVAIDKARIRTIVTAAHADTQLDQA
                     LEAFSKVGRELRLIAG"
     gene            complement(3678..4706)
                     /gene="tdh"
                     /locus_tag="VHM48_15305"
     CDS             complement(3678..4706)
                     /gene="tdh"
                     /locus_tag="VHM48_15305"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX2756837.1"
                     /translation="MRALVKTATHPGLELRDVPEPPMGINDVRIGVRKTGICGTDLHI
                     EAWDAWAARTIKPPLVVGHEFMGDVLEIGSNVTDLEPGDVVSGEGHVVCGHCRHCRAG
                     RWHLCAKTVGLGVNRDGAFAEQVVLPVTNVWHHWPGIDEEVAAIFDPFGNAVHTALSF
                     PVLGEDVLIVGAGPIGLMAAAVVRHAGARFVVVSEPNAFRRELATRMGATHVIDPGAR
                     TLEELQAEIGMVEGFDVVLEMSGNASALRDALAHMAHGGGMAILGIPTEEIPIDVSKV
                     VFNQLTLRGIYGREMYETWYKMSVMLQSGLDIRPAITHRFSYRDHEAAFAAARGGDSG
                     KVILDWND"
     gene            complement(5059..5799)
                     /locus_tag="VHM48_15310"
     CDS             complement(5059..5799)
                     /locus_tag="VHM48_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2756838.1"
                     /translation="MEPRPAVPKPGKIVCVGLNYRDHIAEGAGRDVPTWPLLFAKFAN
                     AVIADGEAIVRPEGTHALDLEVELGVVIGSRARRVAAADAMSHVAGYVVVNDVSARDW
                     QGNKAALAEGQKGDGQWLRAKGSDTFLPMGSVLVAADVLDPAAGLRLRSWRIPGPGRE
                     GAGQPQPMQDGTTLDMIFGIPELIAFISRHITLEPGDVIATGTPSGVGIFRDPPVFLE
                     PGDRVRCEIEGIGSIENPVVDWTDDPRG"
     gene            complement(5816..>6694)
                     /locus_tag="VHM48_15315"
     CDS             complement(5816..>6694)
                     /locus_tag="VHM48_15315"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2756839.1"
                     /translation="ERRSRAGGVGARAGRRERVRPGPAPSFLERYRTAIVTIAAVAIV
                     AVAVGYVFIGSTSAAYTCGNQFAPSPTPPIDPGSSTQLGFLQDDMGNSHNVTPPQRYL
                     YCPPASGTHYNLPGTLGPIPPRLYKPEDKMGPSNWLHNLEHGGLVVLYRNDSTGATAA
                     GQQAFHDYFTGFPDSPICRVPVGNLSPVIARFDDMPHPFAALVWDRVFYFDTWDPALV
                     TRFYLAEAERVDSTGALISPPEKQCAPPSAAPSAAASAPAASGSAPASIAPSVAPSVA
                     PSAPASASPEPSAVPS"
BASE COUNT          971 a         2272 c         2439 g         1012 t
ORIGIN      
        1 ttgcctgcac tgcccgagcc agtgcttccg gcaccgctcc catcgccgtt gtacgctccc
       61 gtgcccgtgc ccgtgcccgt gcccgtgccc gtgcccgtgc ccgtgcccgt gccggtgccg
      121 gtgccggtgc cgccggttgc acccgggttc gaaaccgttc ctccggtcga gccattgcct
      181 gccccggcgc cggtcgcctc gagccggtcg atgacgacct ggttgtggtc gatcgcccgc
      241 tggatcgacg cctcgaccgc cgcggcggcg gtgtcattgc ccttgccctc gagacccgcg
      301 gcgaccgccg tgaggacgac acgatgcttg tcgagagccg ccgccacgag cgcttccagc
      361 gcctgatcgc cggtcgccgc cgcgatcgtc tggtcggcga tctcgcgata ggcgtcgagc
      421 gccgcctgca cggcccgggc gtcgccgcgg acggatgccg agtacgcctc ggcgaggcgg
      481 ctctccaagc ggccgagctc ggcgctggct cgcgaggcgc tgtccgcagg aagggtcgcg
      541 ttctcgaccc acatccgcgt cgcgtagagc ggaccgcccg cctgggcggc ggagatcgag
      601 ccgacggcaa tcccgagcca gacgctcgcg gcgaggagcg gcatcgccat gcgacgggtc
      661 agtgagcggc gcgggatgag ggcgacggac ggcgcaatgt acgccgcgat ccgcgatgcg
      721 tcgatctgca accgggcttc gcgcatgacc cgggcccggg cgcgggcgac ggactgcgga
      781 tccgggctca gccgggcgcg ggcgaacgcc tcgagccgcc gctcgatctc acgatcccgg
      841 acatcgatgg cctgcatgtc gtcatccatc cgtcgttgca ttcgcgcggc gaggggctcg
      901 catctcggcg acatcgctgc cgtcgagctc gatcccgagc tgccgccgga gggcggcgat
      961 ggcccggaac tggagaccgc ggacggtccc ttcctgcttg cccatcgcct cggcggcttc
     1021 ccgggccgat agcccggcga agaaccgcaa cgcgatcgcc tctcgttgtt cgccggtcag
     1081 gcctgccagc gccaccccga cggcgtccag ctcctggcgc acgaccgcga tctcatccgg
     1141 accggcgtcc ccgtgggtcc attcgatgat cgagtccaac tcggcatggt cgtgccgcgt
     1201 ccggacgtgg tcgatgaccg cattcctggc cagccggaag agccagcccc cgaagggaat
     1261 gccccgcgac tcataccgcg gcaacgcttc cagggccttg acgaagacat gctgcgtcag
     1321 gtcctccgcg tccgacgggc ggtggacacg gctggcgatg aaacggtaga tcggctcgtg
     1381 gtactggtcg aagatcaatc cgaacgccca ggcatccccc tgctgggcct cggtcacgag
     1441 ccgttcgatc gctgcgtcgt cgatggtcat aacgacggaa tgccttgcct cgttaggtgt
     1501 tcgccccctc gacacccacc cgggtgcgaa agcgatccgg cagggtcacc ccgaccctgg
     1561 atccgcctcg acggaaggca agctgcccga accccgcggc ggccacgaca ccataccccg
     1621 cctcgaggcc cgctgtgaat gaccgatttc gatgtgcgtc ggaaccgatg acgactctgg
     1681 tgcctccgag caactggtag cgctcgacga tcggggccgg cgggtacgtc tccccggcac
     1741 cctggcggag gccgctcgtg ttgacctcga gcgcaacgcc ggtctcgacg agggcgacga
     1801 ggagcggctc gtacagctcc ggtgcggcgg ccagctgggc gggcgtgacg tgcggggcga
     1861 gatagcgctt cacgacgtcg agatggccga tcgtgtcgaa gaggccggag cggatcgcgt
     1921 cgaggacctc gtcgaagtac ggggcgacga tctcggcgag cggccggccg gcagtccaag
     1981 ccgcaacgtg cgtttcgtgg aacggcgagt cggtgtagac gtgaacgctg ccgatcacgt
     2041 agtcgtagac gtgccgggcg aggtgatcgc ggatgtcggg ctcgtgggcc cggtcgtagg
     2101 tgatctcgac gccgaagcgg atcgcgaccc cgcgcggcgc ccaccgctcc gcggcctccc
     2161 ggaccacccg ttcccgctga tcgaatgtgg cgaacgagaa cgccggcgtg ccgggcacga
     2221 agtcgacgtg atcggtgatc gcgatctccg tgatcccgcg ctcgatcgcc tccgcggcgt
     2281 aggcatcgat cggaacgtcg ctgtcgggcg agaggtccgt gtggacatgg gcgtcgagcg
     2341 gcagatccct cgcctggacg gtgggatctg gcagatgaga gccggcaaca tcggcgttca
     2401 cccgcgcttg ctcaccgagg tcggagggct gagtggctgc acctcagccc gcgatgaggc
     2461 gcagctcacg gccaaccttc gagaacgcct cgagcgcctg atcgagctgg gtgtcggcat
     2521 gggcggccgt cacgatcgtc cggatccggg ccttgtcgat cgcgaccgtc gggaagacga
     2581 ccggctgggc gaagacgccc tcttcgaaga gccgctggct gaagcggccg gccgtgtccg
     2641 ggtcgcccat catcacgggc gtgatcgggg tctcggagcg gccggtgtcg aaccccagcc
     2701 tggccagctc cgccttgaac cgcttcgtgt tggcccacag ccgctcgaga agctccggct
     2761 cctcctgcat gaccttgatc gcctcgcggc aggccgcggc gacggccggc ggatgagagg
     2821 tcgagaagag gaagggtcgg gcgcgctgga cgaggatgtc gcgaagatcc tgcgagccgg
     2881 cgacgtagcc gccgaggacg ccgaccgcct tgctgagggt cccgacctgg atcgcgaccc
     2941 ggccgtggag cccgaagtga tcgaccgaac cgcggccgtc gcgcccgagg acgcccgacg
     3001 cgtgggcgtc gtcgacgaag accgccgcat tggccgactc ggccgcctcg acgatcccgg
     3061 gcaacggtgc gatgtcgccg tccatgctga agacaccatc ggtcacgacg aggatcagcc
     3121 gatacgggag cccagtcccc tcgcgaccct tctcgaccgc ctcgtcgagg accgcccgga
     3181 ggctgtcggt gtcggcgtgc ttgtagatct tccggggcgc cttcgagagc cgcatcccgt
     3241 cgatgatcga ggcgtggttc agctcgtcgc tgacgatcag gtcctgctcg ccggtgatcg
     3301 tcgggatgac gccggtgttg gcgctgaagc cggactggaa cgtcagcgtc gcggcggtgc
     3361 ccttgaactc ggccagctcg cgctcgaggt cctcgtggag ggtcatcgtc ccggcgatcg
     3421 tccggaccgc gccggagccc gccccgaagt cgcgaacggc gtcgagcgcg gccttgcgca
     3481 ggcgcgggtg gtgggtcagg ccgaggtagt cgttcgagga caggctgatc aggcggtgct
     3541 cgtcgaccga gacgatcggg ccctgggccg acgacatcac ccggagcggc cggtagagat
     3601 ggcgatcgcg aaggtcggcg agctcctcgc caaggaaggc cagtggatcg ggccggaggt
     3661 cggtgctcat tgctgcctca gtcgttccaa tcgaggatca ccttgccgga atcgccgccg
     3721 cgcgcggcgg cgaacgccgc ctcgtggtcg cgatacgaga agcggtgggt gatggccggg
     3781 cggatgtcga gcccggactg gagcatgacc gacatcttgt accaggtctc gtacatctcc
     3841 cggccgtaga tgccgcggag cgtcagctgg ttgaagacga ccttgctgac gtcgatcggg
     3901 atctcctcgg tcgggatgcc gaggatcgcc atcccgccgc cgtgggccat atgggccagc
     3961 gcgtcgcgaa gtgcgctggc gttgccggac atctcgagga cgacgtcgaa cccctcgacc
     4021 atgccgatct cggcctgcag ctcctcaagc gtccgtgcac ccgggtcgat gacgtgcgtc
     4081 gcccccatcc gggtcgcgag ctcgcggcgg aacgcgttgg gctcgctcac gacgacgaag
     4141 cgggcgcccg catgacggac gaccgcggcg gccatcagcc cgatcgggcc ggccccgacg
     4201 atgaggacgt cctcgccgag gacggggaag ctgagcgccg tgtggaccgc gtttccgaac
     4261 gggtcgaaga tcgccgcgac ctcctcgtcg atcccgggcc agtggtgcca cacgttcgtc
     4321 acggggagga cgacctgttc ggcgaacgca ccgtcgcgat tgacgccgag cccgaccgtc
     4381 ttcgcgcaga ggtgccagcg gccggcccgg caatggcggc aatggccgca gacgacatga
     4441 ccttcgccgc tgacgacgtc gccgggctcg aggtcggtca cgttgctgcc gatctcgagc
     4501 acgtcgccca tgaactcgtg gccgacgacg agcggcggct tgatcgttcg ggcggcccac
     4561 gcgtcccacg cctcgatgtg gaggtccgtg ccgcagatcc cggtcttgcg gaccccgatc
     4621 ctgacgtcgt tgatgcccat cggcggctcc gggacgtcgc gcaactcgag gcccggatgg
     4681 gtggcagtct tgaccagggc tcgcatcagg cgctcagtgt gccgtgtcgc gacgatgccc
     4741 gcccgtgccg atcggatcgg gctgtcgcgg cggttcggtt gacccgggcg gcgttgccga
     4801 gcctgactca ccttccgagt caagatcggg accaccgtgc gggctccgtc gcgattcctg
     4861 cgcgaccgcg agggcttgtg gtcgatctga ctcggcggat gagtcggatc gacacgagcg
     4921 gcccttccgg gtcggccggc gtgtcatgca gcccgtgacc ccgtcgtgac tcagcggaga
     4981 gcggagctcg gcgggcgttc accggcatgg cgggcagcgt tcgccggcgg gcagcgttcg
     5041 ccggcggtca ggcgtccctc agccccgcgg gtcgtccgtc cagtcgacga ccgggttctc
     5101 gatcgaaccg atcccttcga tctcgcaacg gacccggtcg cccggctcga ggaagaccgg
     5161 cggatcgcgg aagatgccga cgccggacgg cgtcccggtg gcgatgacat cgccgggctc
     5221 gagggtgatg tggcggctga tgaaggcgat cagctccggg atcccgaaga tcatgtcgag
     5281 ggtcgtgccg tcctgcatcg gctggggctg gccggcgccc tcccggcccg ggccggggat
     5341 ccgccagctc cggagccgga ggccggcggc cggatcgagc acgtccgcgg cgacgaggac
     5401 cgaacccatc ggcaggaagg tgtcgctgcc cttggcccgg agccattggc cgtcgccctt
     5461 ctggccctcg gcgagggcgg ccttgttgcc ctgccagtcg cgggccgaga cgtcgttgac
     5521 gacgacatag cccgccacat ggctcatcgc gtcggctgcg gcgacccgcc tggcgcggct
     5581 cccgatcacg acgccgagct cgacttcgag atcgagggcg tgggtgccct cgggtcggac
     5641 gatcgcctcg ccgtcggcga tcacggcgtt tgcgaacttg gcgaagagga gcggccaggt
     5701 cgggacgtcg cgcccggcac cctcggcgat gtgatcgcga tagttcaggc ccacacagac
     5761 gatcttgccg ggcttcggga cggccggccg gggctccatg gcgccgcgcc gcccgtcagc
     5821 tcgggaccgc ggacggctcc gggctcgcgc tcgccggcgc cgagggtgcg accgatggcg
     5881 caacggacgg ggcgatcgac gcgggcgccg agcctgatgc cgccggggcg ctcgccgcgg
     5941 ccgacggggc agcggacggc ggcgcgcact gcttctccgg cggcgagatg agtgccccgg
     6001 tcgagtcgac ccgctcggcc tcggccaggt agaaccgggt cacgagcgcc gggtcccagg
     6061 tatcgaagta gaagacgcgg tcccagacaa gcgcggcaaa cggatgcggc atgtcgtcga
     6121 agcgggcgat gaccggcgag aggttaccga ccggaaccct gcagatcgga ctgtcgggaa
     6181 agcccgtgaa gtagtcgtgg aaggcctgct gcccggccgc ggtggcgccc gtcgagtcgt
     6241 tccgatagag gacgacgagg ccgccgtgct cgaggttgtg gagccagttg gacggtccca
     6301 tcttgtcctc cggcttgtag agccgaggag ggatcgggcc gagggtgccg ggcaggttgt
     6361 agtgggtgcc ggaagcgggc gggcagtaca ggtacctctg gggtggcgtg acgttgtggc
     6421 tgttgcccat gtcgtcctgg aggaacccga gctgggtgct cgagccggga tcgatcgggg
     6481 gcgtcggcga gggggcgaac tggttgccgc aggtgtaggc ggccgacgtc gagccgatga
     6541 agacgtagcc gaccgcgacc gcgacgatcg ccacggctgc gatcgtgacg atcgcggtcc
     6601 gatagcgctc gaggaacgac ggagccgggc cggggcggac gcgttcgcga cgtcctgcgc
     6661 gagcgccgac gccgccggcc cggctccgtc gttc
//
LOCUS       DATAIW010000521         9405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_268066, whole genome shotgun sequence.
ACCESSION   DATAIW010000521 DATAIW010000000
VERSION     DATAIW010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268066"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHM48_15320"
     CDS             complement(<1..763)
                     /locus_tag="VHM48_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2756840.1"
                     /translation="MTDTLEAVKTHQQFIGGEWADAASGETLDVINPANDQVIAKVPK
                     SASEDVDRAVEAADKAFPAWGGATPAERSNALLKLADALEARGEEIGRLESQNAGKPV
                     GAAIDEVPVVVDNLRFFAGAARLLEGKAANEYMAGHTSMIRREPVGVVASIAPWNYPI
                     MMAGWKIGPALAAGNTVVLKPSARTPLTALVLAEIAADILPPGVLNVITGTGAGIGDA
                     LVGHPKVGMISVTGDTETGKHIARTAADKVKRLHLE"
     gene            complement(901..1719)
                     /locus_tag="VHM48_15325"
     CDS             complement(901..1719)
                     /locus_tag="VHM48_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2756841.1"
                     /translation="MTDGAEPTVGTTARRFGPPLALVASLLGAWELYARTSGVSPFVL
                     PAPTQVLAALWDFREQAIRHAVPTIVETIVGFSLSIAGATAVAVALDRVAWARRTVEP
                     LLVGSQTIPIIAIAPLIVVWFGFGLLPKVLVVVLVTFFPITISLLDGFASTAAEASEL
                     MRSFGASAGETFRKIRWPSALPAFFTGLRISATYAVVAAVIGEYVGATDGLGIWMQLS
                     QRSFRTDLVFGAILLTAILSVGLYGLVVLAERATIPWWRASRAAGSAGSRSVEA"
     gene            complement(1716..2795)
                     /locus_tag="VHM48_15330"
     CDS             complement(1716..2795)
                     /locus_tag="VHM48_15330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2756842.1"
                     /translation="MKVLRLLAAMLVIAAGCGPSNQSASPSDAASASASPVATGQVNT
                     SPSARPTPATVRLALDWTPNTDHTGFFVARAKGWYELAAIDLQILPYGTLAPENLLAA
                     HQAECGISFQDSMTFAVAAGADIVSVAAILQKTASAIGVLANGPIQRPRDLDGKTYGS
                     FAYPAEVPTMKAVIKADGGKGAFKVAILDAAAYEALYNKQVDFTIPFTAWEGVEAQER
                     GIKLRYFQFVDYGFPEFYQVVLACDRQWLAKDPDAAKRFVAATVKGFQFAADHADEAA
                     AILVTENPGVFDTNKDLPKASQEFLDSGHYLVDANGVFGTQTLERWTGYSAFLFDQGL
                     LVDAAGKPLPIALDYGTLFTNDFLP"
     regulatory      complement(2860..2965)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2996..3880)
                     /locus_tag="VHM48_15335"
     CDS             complement(2996..3880)
                     /locus_tag="VHM48_15335"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2756843.1"
                     /translation="MATGTDSRAAPAADAPSTTERLAARDGTELLVRHWPASGVPWAS
                     VLLLHGVAEHSGRYEHVGRQLAAAGLEVEAYDQRGFGGSGGRRAFVERWSQNHDDLED
                     RLAAVRASAGNTPVVIFGHSLGALLALGYAVAEPPRPLPDALVLSAPALDSTIPAWKR
                     ALARVLSSVAPTKEMNNDFDGALLSRDPAIGEAYVADPLNQHSTTFRFGAAAFAEQAR
                     VRAALGRLSVPTFVYHGEDDHLVPTDASAPLAAVPGVRRRTYPLFRHETHNEPEGPAV
                     IADVIAWLRATLPSSEGD"
     gene            3921..4733
                     /locus_tag="VHM48_15340"
     CDS             3921..4733
                     /locus_tag="VHM48_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2756844.1"
                     /translation="MSIWTEVGLGVFVRRYQFYDQNIVAIRGDDGFLVVDTRVSHRQA
                     DEILADLRSLSALPVRAVVNTHGHSDHAFGNARFRPAPIWGHTRCATMVRDTGDRQRA
                     GTAAAIPELAEELAEVVLDPPDRLFDTATTVPFDAGGRVVELRYLGRGHTDNDIVVLI
                     PDADVLLAGDLLENDATPNFGDGFPLDWPVTVEALVDLVTGAVVPGHGSVGDQAFAVR
                     QMTEFRAIAELAALVDRGVIGLDDAVLRTPYPTDAAREPLERALAQLRGELD"
     gene            complement(4988..5998)
                     /locus_tag="VHM48_15345"
     CDS             complement(4988..5998)
                     /locus_tag="VHM48_15345"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEX2756845.1"
                     /translation="MSQIPAAFRAFVTEKTEDGVTRGVRSFAAADLPDGAVEVRVDWS
                     SVNYKDALATIADGRVARISPLIPGIDLAGEVISSTDPAVAAGTAVLAHGYDLGVARH
                     GGYGAFTRLPAGYVVPLPVGLKARAAMAVGTAGFTAAMSVAALERHGLRAGDGPVLVT
                     GASGGVGSVAVAILAARGHEVWAATGKPDEEPRLRGLGAAGLVPRDECTAESPRPLES
                     ARWAGAVDTVGAATLPYVLRTLRPGGAVASSGNAGGAALQTTVLPFILRGVALLGMDS
                     ANMPIADRRALWARIATDLRPNGLDDDAGVTEVALADVESALEAILAGSARGRWVVRV
                     GE"
     gene            6404..7741
                     /locus_tag="VHM48_15350"
     CDS             6404..7741
                     /locus_tag="VHM48_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX2756846.1"
                     /translation="MAELDPATFRPETKALHSGQKPDSATNARAVPIYATTSYVFNDA
                     DHAARLFGLQEFGNIYTRIMNPTNDVFEQRVAALEGGVAALAVASGQAAETLSILNIA
                     RAGDNIVSSSSLYGGTWNLFTHTLPKIGITTTYVDGSDPSAFGRAINEKTKAVFLELI
                     GNPRLDVHDLASIADVAHARGVPVIVDNTFAPLLAQPIRHGADIVIHSATKWIGGHGT
                     AIGGVVVDGGTFDWAASERFKQDFVDPDPSYHGISYTAAFGNLAFILKLRVQGLRDIG
                     AALSPFNAFLFLQGLETLPLRIKRHSENALAVAQWLEKRDGVTWVSYPGLESHPSHKQ
                     ATKYLKGGFGGVVTFGVRGGAEAGRNLIDNVTIFSLLANVGDAKSLIIHPASTTHSQL
                     TAEEQESTGTTPDLIRLSVGLEHIDDLIADLDRALSIALGEASTAGDLVGAAG"
     gene            7738..8886
                     /locus_tag="VHM48_15355"
     CDS             7738..8886
                     /locus_tag="VHM48_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2756847.1"
                     /translation="MTQTVGGVAAAPPTAPPVFGSGRLETTPLGPFVLGDGTRLPELV
                     VAWRHDGLPPGEGPQVVVVHALTGSADAAGDWWEPLIGPGRALDTRRIGVLGANLLGG
                     RYGSTGPISIDPRTDRPFGRGFPAVSTRDQARAQWALLDAIGITEVALVVGGSLGGMV
                     ALEVALIRPPAVRHVMPIAAPAATGPMAVAWNHLQVALIERLGDDGLALARQLAMTTY
                     RSETDFEERFGRTSEPDGRPSIVSYLAHQGGKLVDRFDGDTYRILAGAMDRHDIGAGR
                     GGLEAALAYLAVAGVTLTGVGIVDDILYGPRQVRALVTAARAAGVVTRYREIRSTKGH
                     DAFLVEWDQLGTILGESLDRGRPPGTSAGSNGTADAGSYVSDWQGLED"
     gene            complement(8922..9275)
                     /locus_tag="VHM48_15360"
     CDS             complement(8922..9275)
                     /locus_tag="VHM48_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2756848.1"
                     /translation="MGNPIVHFEIRSDDPDAARDFYAKLFGWTYPPGGFPGYTYVDSG
                     VPAGTIPGGISPLQGGASLVTVFAGVADVKAALDQAVALGGTIVQPATQAPGVTFGLF
                     ADPQGHVVGVASNDA"
BASE COUNT         1396 a         3357 c         3311 g         1341 t
ORIGIN      
        1 gctccaagtg gagacgcttg accttgtcgg cggccgttcg cgcgatgtgc ttgccggtct
       61 ccgtgtctcc ggtgaccgag atcatcccga ccttcggatg gccgacgagc gcgtcgccga
      121 tcccggcgcc ggttccggtg atcacattga gcacgcccgg gggcaggatg tccgcggcga
      181 tctcggcgag gacgagggcg gtcaggggcg tccgcgccga gggcttgagg acgacggtgt
      241 tgccggctgc gagggccggg ccgatcttcc agccggccat catgatcggg tagttccacg
      301 gcgcgatcga ggcgacgacc ccgaccggct cgcgccggat catcgaggta tggccggcca
      361 tgtactcgtt ggccgccttg ccctccagga ggcgggcggc gccggcgaag aaccgcaggt
      421 tgtcgacgac gacggggacc tcgtcgatgg cggcgccgac cggcttgccg gcgttctggc
      481 tctcgagccg gccgatctcc tcgccgcgcg cctcgagcgc atcggccagc ttgaggagcg
      541 cgttggaacg ctcggcgggg gtggcgccac cccacgccgg gaaggccttg tcggcggctt
      601 cgaccgcccg gtcgacgtcc tcggacgcgc tcttcggaac cttcgcgatg acctggtcgt
      661 tggccgggtt gatgacgtcg agcgtctcgc cggacgcggc gtcggcccat tcgccaccga
      721 tgaactgctg gtgcgtcttg acggcctcga gcgtgtcggt catggctgga ctccctgtcg
      781 gtgacgtcga cgggccggac ggcgccggac ccggaaggct ggaccgagga ctggtccggt
      841 cattgtgccc ccgaccgacc ggctcgtcgc agtcgccggc tcgccgtccc acgcggcggg
      901 tcaggcttcg acagaccggg agcccgctga gccggcggct cgtgacgccc gccaccacgg
      961 gatcgtcgct cgctcggcga ggacgaccag cccgtacagg ccgacgctca ggatcgccgt
     1021 cagcaggatc gcgccgaaca cgagatcggt ccggaacgaa cgctgactga gctgcatcca
     1081 gatcccgagc ccgtcggtcg ccccgacgta ctcgccgatg accgcggcaa cgacggcgta
     1141 cgtcgccgag atccggagcc cggtgaagaa cgcaggcagc gcggacggcc agcgaatctt
     1201 gcggaaggtc tcgccggccg acgctccgaa cgaacgcatg agctcgctcg cctcggccgc
     1261 cgtcgaggcg aacccatcga ggagcgagat cgtgatcggg aagaacgtga ccagcacgac
     1321 cacgaggacc ttgggcagca gcccgaaccc gaaccagacg acgatgagcg gcgcgatcgc
     1381 gatgatcggg atcgtctgcg aaccgaccag gagcggctcg acggtgcggc gagcccaggc
     1441 cacccggtcg agagcgaccg ccacggccgt tgcgccggcg atcgagaggc tgaacccgac
     1501 gattgtctcg acgatcgtcg ggactgcgtg gcggatcgcc tgctcacgga agtcccacag
     1561 cgcggcgagc acctgcgtcg gcgccggcag cacgaacggg ctcacgcccg aggtccgggc
     1621 gtagagctcc caggcgccga ggaggctggc gacgagcgcg agcggcggcc cgaaccgacg
     1681 ggccgtcgtg ccgacggtcg gctcggcgcc gtcggtcatg gcaggaagtc gttcgtgaac
     1741 aacgttccgt agtcgagggc gatcgggagt ggcttgccgg cggcgtcgac gaggagtccc
     1801 tggtcgaaga ggaacgccga gtacccggtc caccgttcga gtgtctgcgt cccgaacaca
     1861 ccgttcgcgt cgaccaggta gtgcccgctg tcgaggaact cctgcgaggc cttgggcaga
     1921 tccttgtttg tgtcgaacac gcccgggttc tcggtgacga ggatcgccgc cgcctcgtcg
     1981 gcgtgatctg ccgcgaactg gaagcccttg accgtggccg cgacgaaccg cttcgccgcg
     2041 tccggatcct tcgcgagcca ttgccggtcg caggcgagga cgacctggta gaactccggg
     2101 aagccgtagt cgacgaactg aaagtagcga agcttgatgc cgcgctcctg cgcctcgacg
     2161 ccctcccagg cggtgaacgg gatcgtgaag tcgacttgct tgttgtagag ggcctcgtac
     2221 gcggcggcgt cgaggatcgc gaccttgaag gcgcccttgc ccccgtcggc cttgatgaca
     2281 gccttcatcg tcggaacctc ggccggatag gcgaagctgc cataggtctt gccgtcgagg
     2341 tcgcgtggcc gctggatcgg cccgttggcg aggacgccga tggcggacgc ggtcttctgg
     2401 aggatcgcgg cgacgctgac gatgtcggcc ccggcggcga cggcgaacgt catcgagtcc
     2461 tggaagctga tcccgcattc ggcctggtga gcggccagca ggttctcggg ggcgagggtc
     2521 ccgtagggca ggatctggag gtcgatcgcg gcgagctcgt accacccctt cgcccgggcg
     2581 acgaagaacc cggtgtggtc ggtgttgggc gtccaatcca gggcgagccg gacggtcgcg
     2641 ggggtcggcc gtgccgacgg tgaagtgttc acctggcccg tggcgaccgg actcgcggag
     2701 gccgacgcgg cgtcggatgg cgaggccgac tggttggatg ggccgcagcc ggcggcgatg
     2761 acgagcatgg ccgccaggag acgaaggacc ttcacggaga ctcgacctcg ggttctggta
     2821 cagaacgatg ccgccccacg gaaaccgggg cggcagcatc gagtctcatg ggcctgcttc
     2881 cttcgctggg atgaaccaga tcaggttcgg cgggtctgtg gccttgccac actctcagcg
     2941 catttgcgct ccccggaggc gatattcagt tgccgcgagt ctagcaccga ggctgtcaat
     3001 ctccctcgga tgacgggagg gtcgcgcgca gccaggcgat gacgtcggcg atgaccgccg
     3061 gcccctcggg ctcgttgtgg gtctcgtggc ggaacagcgg gtaggttcgg cgacggacgc
     3121 cgggaacggc ggcgagcggg gcgctggcgt cggtcgggac gagatgatcg tcctcgccgt
     3181 ggtagacgaa ggtcgggacc gagaggcggc cgagcgcggc gcggacccgc gcctgctcgg
     3241 cgaaggcggc agcaccgaag cggaatgtcg tcgaatgctg gttgagcgga tcggcaacgt
     3301 acgcttcacc gatcgccgga tcgcgcgaca ggagcgcgcc gtcgaagtcg ttgttcatct
     3361 ccttcgtcgg cgcgaccgag ctcaggaccc gggccagggc gcgcttccac gcagggatcg
     3421 tcgaatcgag ggccggggcg ctcaggacga gtgcgtccgg aagcggccgg ggcggctcgg
     3481 cgacggcgta gccgagtgcg agaagcgcac ccagcgagtg cccgaagatg accaccggcg
     3541 tattgccggc cgaggctcgg accgcggcga gccgatcctc gagatcgtca tggttctggc
     3601 tccagcgctc gacgaatgcc cgccggcccc cggaaccgcc gaacccgcgc tggtcgtagg
     3661 cctcgacctc gagaccggcc gccgcaagct gccgcccgac gtgctcgtag cggcccgagt
     3721 gttcggccac gccgtggagg aggaggacgg acgcccaggg cacgccgctc gccggccaat
     3781 gccgcacgag cagctccgtg ccgtcgcggg ccgcgaggcg ttcggtggtc gagggcgcgt
     3841 cggccgccgg agccgcgcgg gagtccgtcc ctgtcgccat ggtccccatc gtaggcctgt
     3901 cgacaggtac gctgcggcgc atgtcgatct ggaccgaggt cgggctcggc gtcttcgtcc
     3961 ggcgctacca gttctacgac cagaacatcg tcgcgatccg cggcgacgat gggttcctcg
     4021 tcgtcgacac gcgggtcagc catcgccagg ccgacgagat cctcgccgac ctgcggagcc
     4081 tctcggccct gccggtgcgt gccgtcgtga acacgcacgg ccactccgat cacgcgttcg
     4141 gcaacgcccg cttccggccg gccccgatct gggggcatac ccgctgcgcg acgatggtcc
     4201 gcgacaccgg cgaccgccaa cgggcgggca ccgccgccgc gatccccgag ctcgccgagg
     4261 agctggccga ggtcgtcctc gacccaccgg accggctttt cgacacggcg accaccgtgc
     4321 cgttcgacgc cggcggccgg gtcgtcgagc tgcgctacct gggccgcggc cacaccgaca
     4381 acgacatcgt ggtcctgatc cccgacgccg acgtcctgct cgccggcgac ctcctcgaga
     4441 acgacgcgac ccccaacttc ggcgacgggt tcccactcga ctggccggtc accgtcgagg
     4501 cgctggtcga cctcgtcacc ggcgccgtcg tgccgggcca cggcagcgtc ggcgatcagg
     4561 ccttcgcggt ccgccagatg acggagttcc gggcgatcgc cgagctggcg gcgctcgtcg
     4621 accgaggtgt catcggcctg gacgacgccg tcctgcggac cccgtacccc accgacgcgg
     4681 cacgcgagcc gctcgaacgg gccctcgccc agctgcgcgg tgagctcgac tgacttccgg
     4741 gcgatccggg ccaggcgcag tcggcccgct cgcgccgttc attggatgaa ccgatccgac
     4801 cgcgcgacgc gtccacacgc ccgcggccgt caggagtggc gcaccagatg aaccgtatcg
     4861 acggattcgg tcccttccac cgcccgagac acctcatcga aggtggacca cgtcgcatag
     4921 gttcatctga tgcacgcgat atcggggatc ggtcatcaga tgagcgaaac tcgcgtgcgg
     4981 cggccgccta ctcccccacc cggaccaccc agcggccgcg cgccgagcct gccaggatcg
     5041 cctcgagagc gctctccacg tcggcgagcg ccacctcggt cacgccggca tcgtcgtcca
     5101 gcccgttcgg tcgcagatct gtggcgatcc gcgcccacag cgcgcgccga tccgcgatcg
     5161 gcatgttcgc cgagtccatt ccgagcagcg cgacgccgcg caggatgaag ggcaggacgg
     5221 tcgtctggag tgcggcgccg ccggcgttcc ccgacgatgc gaccgccccg cccggccgca
     5281 gcgtccggag cacgtagggc agcgtcgcag cgccgaccgt gtcgaccgcg cccgcccagc
     5341 gcgccgactc caggggtcgg gggctctcgg ccgtgcactc atcgcgcgga acgaggcccg
     5401 cggcccccag cccccgcaaa cgcggctcct cgtccggctt tccggtcgcc gcccagacct
     5461 cgtgaccacg ggcggcgagg atcgcgacgg ccacgctgcc gacgccgcca ctcgcgccgg
     5521 tcacgagcac cggcccgtcg ccggctcgca gcccgtgccg ctccagcgcc gcgacggaca
     5581 tcgcggccgt gaagccggcg gtcccgaccg ccatcgccgc ccgcgccttg agacccaccg
     5641 gcagcggaac gacgtagccg gccggcagcc gggtgaacgc gccatatccg ccgtgccggg
     5701 ccacgccaag gtcgtagccg tgggcgagga ccgccgtgcc ggcggccacc gccggatcgg
     5761 tcgacgagat cacctcgccg gccaggtcga tccccgggat caacgggctg atccgggcga
     5821 ccctgccgtc ggcgatcgtg gcgagggcgt ccttgtagtt gacgctcgac cagtcgaccc
     5881 ggacctcgac cgcgccgtcg ggcaggtcgg cggcagcgaa cgagcggacg ccgcgggtga
     5941 cgccgtcctc ggtcttctcg gtgacgaacg ctcggaacgc ggcggggatc tggctcacgt
     6001 cccggactgt agcgcgcggt cggcggcctc cgcccgatgg ttgcatttcg ggtccggggt
     6061 tgacggatcg gcgccggttc ggcgaccatc cgcgggcgat gacccacacc caagtctcct
     6121 ggaacatgtg tatgcgctgg cggccccgcc gtgcggagga tggggtctga ggtctcgcgc
     6181 ataccggaga tgacgggagc gggaccacag acgaggtccc ggggccgcgg ttcctgaagg
     6241 caggagccgc ggcgttttgt ttcctccgag cgacacgacg ggtgaccgcc ggagccccga
     6301 ccgccaccgc cctccccagg gcccgggcct cgcagcgaca tccacccacc ggccggggca
     6361 cgtatcgacc tcgaccggac gtcacacgga aggaagcatc cgcatggccg aactcgatcc
     6421 cgccacgttc cgccccgaga ccaaggcgct ccattccggc cagaagccgg actccgcgac
     6481 caacgctcgg gccgttccga tctacgccac gaccagctac gtcttcaacg acgccgacca
     6541 cgcggcccgg ctgttcgggc tccaggagtt cgggaacatc tacacccgga tcatgaaccc
     6601 gaccaatgac gtgttcgagc agcgggtcgc cgcgctcgaa ggtggcgtcg ccgcccttgc
     6661 ggtcgccagc ggccaggccg ccgagaccct ctcgatcctg aacatcgccc gggcgggcga
     6721 caacatcgtg tcgtcatcgt cgctctacgg cgggacctgg aacctgttca cccacaccct
     6781 gccgaagatc gggatcacga cgacctacgt cgacggcagc gatccgtcgg ccttcggacg
     6841 ggcgatcaac gagaagacga aggccgtctt cctcgagctg atcggcaacc cccggctcga
     6901 cgtccacgac ctcgcctcga tcgccgacgt ggcccacgcc cgcggcgtcc cggtcatcgt
     6961 cgacaacacg ttcgccccgc tcctcgccca gccgatcagg cacggtgcgg acatcgtcat
     7021 ccactcggcg acgaaatgga tcggcggcca cgggaccgcg atcggcggcg tcgtcgtcga
     7081 cggcggcacg ttcgactggg cggcgtccga gcggttcaag caggacttcg tcgacccgga
     7141 tccgtcctac cacgggatca gctacacggc cgcgttcggc aatctcgcgt tcatcctgaa
     7201 gctgcgcgtc cagggcctcc gcgacatcgg tgccgcgctc agcccgttca acgcgttcct
     7261 gttcctgcag ggcctcgaga cgctcccgtt gcggatcaag cggcacagcg agaatgccct
     7321 cgcggtggcc cagtggctcg agaagcgcga cggcgtgacc tgggtcagct accccggcct
     7381 cgagtcgcat ccatcgcaca agcaggcgac gaagtacctc aagggcggct tcggcggcgt
     7441 cgtcaccttc ggcgtccggg gcggcgcgga ggccggccgg aacctgatcg acaacgtcac
     7501 gatcttctcg ctcctggcca acgtcggtga tgcgaagtcg ctgatcatcc acccggcctc
     7561 gacgacccac tcgcagctga ccgccgagga gcaggaatcg accgggacga ccccggacct
     7621 catccggctg tcggtcgggc tcgagcacat cgacgacctg atcgcggatc tcgatcgggc
     7681 cctgtcgatc gccctcggcg aggcctcgac cgccggcgac ctcgtcgggg ccgctggatg
     7741 acccagaccg tcgggggcgt cgctgcggcg cccccgaccg ctcccccggt cttcgggtcg
     7801 ggccggctcg agacgacgcc cctcggaccg ttcgtcctcg gcgacgggac acggctaccg
     7861 gagctcgtcg tggcctggcg ccacgacggg cttccgccag gcgaggggcc ccaggtcgtc
     7921 gtcgtccacg ccctcaccgg ttccgccgac gcggccggcg actggtggga gccgctgatc
     7981 gggccgggtc gtgcgctcga cacccggcgg atcggcgtcc tcggcgccaa cctgctcggc
     8041 gggcgctacg ggtcgaccgg cccgatctcc atcgacccgc ggaccgaccg gccgttcggg
     8101 cgcggatttc cggctgtctc gacgcgcgac caggcccggg cccagtgggc cctcctcgac
     8161 gccatcggaa tcacggaggt agcgctcgtc gtcggcggtt cgctcggtgg gatggtcgcg
     8221 ctcgaagtcg ccctcatccg gccgccggcg gtcaggcacg tcatgcccat cgcggcccct
     8281 gccgcgaccg ggccgatggc cgtcgcctgg aaccatctcc aggtcgcgct catcgagcga
     8341 ctcggcgacg acggtctcgc cctcgcccgc cagctcgcga tgaccacgta ccgcagcgag
     8401 accgacttcg aggagcggtt cggccgaacc tcggagcccg acggtcggcc gtcgatcgtc
     8461 agctacctcg cccaccaggg cgggaagctc gtggaccgct tcgacggcga cacgtatcgg
     8521 atcctcgcgg gggcgatgga ccgccacgac atcggcgcgg gacggggcgg cctcgaggct
     8581 gcgctcgcct accttgccgt cgcgggcgtc accctgaccg gggtcgggat cgtcgatgac
     8641 atcctgtacg ggccgcgcca ggtgcgtgcg ctggtgaccg ccgcacgggc ggccggcgtc
     8701 gtgacccgct accgcgagat ccgctcgacg aaggggcacg acgccttcct ggtcgaatgg
     8761 gaccagctcg ggacgatcct cggcgagtcg ctcgatcgtg gacgcccccc gggcacgtcg
     8821 gccggctcga acgggacagc cgacgcgggc tcgtacgtca gcgattggca gggcctcgag
     8881 gactgatccc cgacagccct gcccgtctcg acctcggtgg ttcaggcgtc gttcgaggcg
     8941 accccgacga catggccctg cggatcggcg aagagcccga acgtcacgcc cggtgcctgg
     9001 gtcgccggct ggacgatcgt gccgcccagg gcgacggcct ggtcgagcgc ggccttgacg
     9061 tcggcgaccc cggcgaagac cgtcacgagc gaggctccac cctggagcgg gctgatgccg
     9121 cccgggatcg tgcccgccgg caccccgctg tcgacgtagg tgtaaccggg gaagccgcct
     9181 ggcgggtagg tccagccgaa cagcttcgcg tagaagtcgc gcgccgcgtc gggatcgtcg
     9241 gagcggatct cgaagtggac gatcgggttg cccatgacgg tggtcctcca gcctgttgcg
     9301 tggcgttcag accatcgacg aacggcgtgg ggcggaatcg acaggcccgt tctccgagcg
     9361 accgggccga cgctcagggc gtgccctggc tggcctggct cgagg
//
LOCUS       DATAIW010000522         9972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_147659, whole genome shotgun sequence.
ACCESSION   DATAIW010000522 DATAIW010000000
VERSION     DATAIW010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147659"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1887
                     /gene="hydA"
                     /locus_tag="VHM48_15365"
     CDS             490..1887
                     /gene="hydA"
                     /locus_tag="VHM48_15365"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX2756849.1"
                     /translation="MRTLITNGTIVTADGSTAADVLVDGETVAQIGSDLAANGVSADE
                     TIDAAGRWLIPGAIDAHTHMELPFGGTFAKDTFESGSRAAAFGGTTTIMDFAVQSKGK
                     ALREGLDTWMAKAEGNTVADYAFHMIMSDVNDATLAEMDLLVDEGIPDFKLFTAYPGV
                     FYSDDGAIFRAMQQTVKNGGLIMMHAENGMAIDVVAGQLAAAGKTDPYYHGVARYPVF
                     EGEATNRVIRLAEAAGAPVYIVHLSSRDALEAVRGARDRGTKAFAETCPQYLFLSLED
                     MGNGFEGAKFVCSPPLRPADHQEELWNGLIKDDLQLVATDHCPFDFHGQKDLGTADFR
                     KIPNGLPGVEDRVDLLHDGGVVGGRISRERWVEIISTAPAKLFGMFPQKGTIAVGSDA
                     DIVVYDPDATRTISASTHHMDCDYSCYEGRAIHGRSDIVLSRGSVIVRDGAFTGRKGH
                     GRYIKRAPADFVRLS"
     gene            1940..2443
                     /locus_tag="VHM48_15370"
     CDS             1940..2443
                     /locus_tag="VHM48_15370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2756850.1"
                     /translation="MPDPGTAPDPLTSPAEPTITIVAGVDQLAAVRAFVRTHVPAAGG
                     DEETTADFVQAVDELVCNVIGHGYAGRPGSVEIAFLETPQEIAFRIRDDAPPFDPTAV
                     PEPPLDLPLAERRLGGMGIHLARTLTDGFDHRILPTGGNEVTVRKQRRITTGEASNGH
                     HDGPTEP"
     gene            2412..2825
                     /locus_tag="VHM48_15375"
     CDS             2412..2825
                     /locus_tag="VHM48_15375"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2756851.1"
                     /translation="MDITTDRPSPDLAVIGLSGELDGSNFEQLIDAGRAARDGGARRI
                     VLDLAGLTYMGSSGLVAIHSIALLLRGQEPTSPEDGWQAIHDLDASGGDGPDAALSLA
                     GPPPSIERVLDRSGMLGLFAVHADRASAVAAATTA"
     gene            2858..4414
                     /locus_tag="VHM48_15380"
     CDS             2858..4414
                     /locus_tag="VHM48_15380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2756852.1"
                     /translation="MRAPRLERLIERAGLDALLEAASAIAPGIEVRILGGDGAEITRS
                     PATDERSATTTATASVRPLVVDGNDLGSVAVRAGDEAAARSIGELVGQAIELAATEGL
                     GRRMVAAAAVDDLRELALLSRLSETLGSAVDPAGIADCVLSTVSRPLGPAVGFVLGPD
                     DATMLAVSGPPDDIAALRADAASLVASLRAENPTIASCAAVGTPSDHRFGTILAAFLR
                     TARGHHGTIVLGRDAGAGEFTAADRQLLASVAGQAAIAIERAGLQRQIVERRALDEEL
                     AIGRRIQLSLMPRRFPTIEGWEIASAYEPAREVGGDFYDVFRLRDRGDCVGLVVADVT
                     GKGIPAAILMADTRGLIHAAADHSDDPAETLARVNRILVDERASGLFVTVVHAVLDPR
                     TGRLVLARAGHDPVHLLRADGRLEVLVPPGRLIGMVADLGVTSVELRVEPGDAFVGHT
                     DGITEARAPDGSFYGEDRFRALLAGLAGTTAAGIVAAVTADVASFRGRAEPSDDLTLL
                     VVRRQPAAAG"
     gene            4551..5675
                     /locus_tag="VHM48_15385"
     CDS             4551..5675
                     /locus_tag="VHM48_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756853.1"
                     /translation="MPADLLLPLFALVLLANAVLVAAAIRGMLRGRPDLDRPTTIGRP
                     NGIGMVASRNLDRPLTDELKHAIAARQGIADEAPLASTPPGQAPLASTPPEPAAPDPE
                     DPSDAGPDNERFSTSQASPARAAAGSSEAMPPATSPPATSPPETPAPRRRRTAQAGIN
                     ADARGPRPADPPRRGRRRFSLPPEEDHEKVNRSIESFLSGIDGSGSDPAAGTADPSSG
                     GPTTVALVAVDGLVDSGRAGLPTATGASTGNDVDDAQSADAVAMVERTLRGAARGSDV
                     VSVDGRGRFRVILRGTGELAARAYLRRIRSTVEPMLEASDRPLRFVVATATVLGEPIE
                     DAVRQADRRLAATLGATRDAEGATGQDHGEPAFDPRAAGD"
     gene            complement(5680..6546)
                     /locus_tag="VHM48_15390"
     CDS             complement(5680..6546)
                     /locus_tag="VHM48_15390"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX2756854.1"
                     /translation="MPTAALAFDFDPLLHLGDGAVRWETVAIAASIFGAIVLAGVAAR
                     ASGLRVDDLLFVVLGIVPGAVIGGRLGYVLLHPAFFTAVPGRILDPGVGSLELTVGVV
                     GGALTGSLVALLLDGRPGPWLRACTFPTLFLLVAGKLAMVLGGTGQGLPTSGEPATAY
                     LGSGPWGSLGPAVASIPSQALEAIATAIVVVVIVLASLLPQLRRSDGRLFLFALAGWA
                     VGRAVVASTWRDPVVAGQLRAEQVIAAVVAIGSLALAIVFMAIHRGQPEEEPAPVGTP
                     DGAPVGPVGPVG"
     gene            6874..7677
                     /locus_tag="VHM48_15395"
     CDS             6874..7677
                     /locus_tag="VHM48_15395"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2756855.1"
                     /translation="MSAHSTLAIRLLGRVDVHADDVTIRIGGRHAQALMALLALRPRP
                     RLRDTLAAELWPEAGAPSAASLRQALWLIRSSLTAAGVDADQWLEADQDTIGLHRDAA
                     LDLDVDSFERLATSGAPDDQEHALRVYAGDLLEGLGHECFAADRERLSDLYEDLLAEV
                     AQNRLDHGDTVGARRAASELLIRDPLREEAHAVLIAAYGRTGSRSQVVRQYRRVCAIL
                     RAELAVAPLPETDAAYRVALAVTATRSRDRAAEVRYDAGPFAPVLVSSA"
     gene            complement(7816..>9972)
                     /locus_tag="VHM48_15400"
     CDS             complement(7816..>9972)
                     /locus_tag="VHM48_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2756856.1"
                     /translation="SNAILDKTSAIVVLKQQPVATYDGHVPGYLKTRVSSAKLNPNSA
                     AAKKYVGYLKTEHSAFAKWLQTNVPSAKITSQYFVTLNAVAVKLNGAAIGKLANNTDV
                     AVVEYNALYHPDMSESYKLINAQAAWAAAGGRADAGAGVKVGMIDSGIDYRHPFFDPA
                     GFAYPAGFPKCDAADMTGSTCKNVSEKVIVAKVYYNRNNQTDYDALPVAGEGDHGSHT
                     AGTVAGVTGKTATVEGVAIDDMSGIAPGAWLGNYNVFPGAVVNARSEDILNAVEAAVI
                     DGMDVLNLSLGGAYHGNNDLLAKGLDTAVAAGVVVVASAGNEGPGGFTIGSPGRARDI
                     ITVGASTNNHFVGQPLTYTGDSSATVGAAVGDFGSMPAGTFGLFDTAANGCAAIAAGA
                     SGKVALINRGVCTFSQKVANAKAAGATGVIVVNNVAGDPTAMARSVGFDDDIPAVMIS
                     NTAGAALRAAHATSVTVAATFSEFVTSNGDILAGFSSQGPTNVDYAIKPDLASVGVNV
                     LSSVACTNGASCGGEGDWAFFSGTSMSAPHVAGSAAVLRGLHPTWTPAQIKSALVNTA
                     DLVVKNAFDASTTVGPQAQGAGREDLAEAASTDATFWPTSASFGRINASKTNATSIAV
                     KVTNLTGTARTFDVAELKFTPAGGALAAFNGGTIGGDDSRITTPSSITVAANSSATLT
                     ITVNAGLANGTIAQGWIQLSGGGDEYQVAYWAQVAP"
BASE COUNT         1386 a         3648 c         3503 g         1435 t
ORIGIN      
        1 cgcggatcac gatcccaccg acgtcgacga acccgcttcc gtcccctggt cgttcgggcg
       61 acggcggtct agcggcgatc ctggcgacga cgggcctcgg cctcatcgcg ctcctgcccg
      121 ccctgcgccg ccgccgccgc cgaccctgac ctgcgccggc gagcccggcc tgcatcgttg
      181 cgccgtgcgg tgctcaccga gtgcgcgttg cgacgcctga gggcgtcacc aaccttgccg
      241 atcgagcgtt cgtccgtcgt cctgcgcagt gcctgagcgg atcagcgcgt gagcgggctc
      301 gcgttcgcgg gcgccgggtg atccttcgtc acaacgcgca cgtcgtgcgc acgcgggaac
      361 ccggtcgcgg gcgggagccc ggtcgcggac ggagctgacc gacgccgccg ccgaccctga
      421 cccgcgccgc ttcgctatcc tcgttgcctg ccccgctcgc cgctcgcggc cgggcctgga
      481 ggaggaccga tgcgcacgct gatcacgaac gggacgatcg tcaccgccga cggctcgacc
      541 gccgccgacg tcctcgtcga cggcgagacc gtcgcccaga tcgggtcgga cctcgcggcc
      601 aacggcgtgt cggccgacga gaccatcgac gccgccggtc gctggctcat cccgggcgcg
      661 atcgacgccc acacccacat ggagctgccg ttcggcggga cgttcgccaa ggacacgttc
      721 gagtccggca gccgggcggc cgcgttcggc gggacgacga cgatcatgga cttcgccgtc
      781 cagtccaagg gcaaggcgct ccgcgagggc ctcgacacct ggatggccaa ggcggagggc
      841 aacaccgtcg ccgactacgc cttccacatg atcatgagtg acgtcaacga cgccaccctc
      901 gccgagatgg acctcctcgt cgacgagggc atccccgact tcaagctctt caccgcctac
      961 cccggcgtct tctacagcga cgacggggcg atcttccggg cgatgcagca gaccgtgaag
     1021 aacggcgggc tgatcatgat gcacgcggag aacgggatgg cgatcgacgt cgtcgcgggc
     1081 cagctggccg cggcgggcaa gaccgatccc tactatcacg gcgttgcccg ttatccggtg
     1141 ttcgagggcg aggccaccaa ccgggtgatc cgcctcgccg aggccgccgg tgcgccggtc
     1201 tacatcgtcc acctctcctc gcgcgacgcg ctcgaggccg tccgcggcgc ccgcgaccgc
     1261 gggacgaagg ccttcgccga gacgtgcccg cagtacctgt tcctgtcgct cgaggacatg
     1321 ggcaacgggt tcgaaggcgc caagttcgtc tgctcgccgc cactccgccc ggccgatcac
     1381 caggaggagc tctggaacgg actgatcaag gacgacctcc agctcgttgc gaccgaccat
     1441 tgcccgttcg acttccacgg ccagaaggat ctcggcacgg ccgacttccg gaagatcccg
     1501 aacggcctgc ccggtgtcga ggaccgggtc gacctgctcc acgacggcgg cgtcgtcggc
     1561 ggccggatca gccgggagcg ctgggtcgag atcatctcga cggcgcccgc gaagctgttc
     1621 gggatgttcc cgcagaaggg cacgatcgcg gtcggctccg acgcggacat cgtggtctac
     1681 gaccccgatg ccacccggac gatcagcgcg tcgacccacc acatggactg cgactactcg
     1741 tgctacgagg gacgggcgat ccacggtcgc tccgacatcg tcctgagccg cggctcggtg
     1801 atcgtccgcg acggcgcgtt caccggtcgc aagggccatg gccggtacat caagcgcgcc
     1861 ccggccgatt tcgtccggtt gagctgaccc gatccgaccg cgttcaggcc ccgttccagc
     1921 tgccgatcga gccgccacga tgcccgaccc ggggaccgca cccgacccgc tgacatcgcc
     1981 cgccgagccg acgatcacga tcgtggccgg cgtcgatcag ctcgccgccg tccgcgcctt
     2041 cgtccggacc cacgtccccg ccgccggcgg ggatgaggag accacggccg acttcgtcca
     2101 ggccgtcgac gagctcgtct gcaacgtcat cgggcatggc tacgccggcc gaccgggttc
     2161 ggtcgagatc gccttcctcg aaacgcccca ggagatcgcg ttccggatcc gcgacgatgc
     2221 gccgccgttc gatccgaccg ctgtcccgga gccgcccctc gatctgccgc tggcggagcg
     2281 ccgcctcggc ggcatgggca tccatctcgc ccggaccctg acggacgggt tcgaccaccg
     2341 tatcctgccg acaggcggca acgaggtcac ggtccgcaag cagcgtcgaa tcaccacggg
     2401 agaggcgagc aatggacatc acgacggacc gaccgagccc tgacctcgcg gtcatcggcc
     2461 tcagcggcga gctcgacggc tcgaacttcg agcagctcat cgacgccggg cgggcagctc
     2521 gcgacggggg cgcccggcgg atcgtgctcg acctcgccgg cctgacctac atgggcagct
     2581 cgggtctcgt cgcgatccat tcgatcgccc ttctcctccg cggccaggag ccgacctcgc
     2641 ccgaggacgg ctggcaggcg atccacgacc tcgacgcctc cggtggcgac ggcccggacg
     2701 ccgccctgtc cctggccggt ccgccaccgt cgatcgagcg ggtcctcgac cgcagcggaa
     2761 tgctcggcct gttcgcggtc cacgccgacc gggcgtcggc cgtcgcggcc gcgacgaccg
     2821 cctgaggctg tcgcccgaac gcggaaggat gaccgccatg cgtgctccga ggctcgagcg
     2881 gctgatcgag cgggccggtc tcgacgcgct gctcgaggcg gcgtcggcca tcgcgcccgg
     2941 catcgaggtg cgcatcctcg gcggcgacgg tgccgaaatc acgcggtccc ctgcgaccga
     3001 cgagcggagt gcgacgacga cggcgacagc ctccgttcgg cccctggtcg tggacggcaa
     3061 cgacctcggg agcgtggcgg ttcgcgctgg cgacgaggcg gcggcgcgct cgatcggcga
     3121 gctggtcggc caggccatcg agctcgccgc gaccgaaggc ctcggccgtc ggatggtcgc
     3181 cgcggccgcg gtcgacgacc tccgcgagct tgccctcctg tcgcgtctgt cggaaacgct
     3241 cggatccgcg gtcgatccgg ccggcatcgc cgattgcgtc ctctcgacgg tgtcgcgccc
     3301 gctcggtccc gcggtcgggt tcgtcctcgg ccccgatgac gcaaccatgc tggcggtcag
     3361 tggcccgccc gacgacatcg cggccctgcg ggccgacgcc gcatcgctgg tcgcgagcct
     3421 ccgggccgag aacccgacga tcgcctcctg cgccgcggtc gggacaccgt ccgaccatcg
     3481 attcgggacg atcctcgcgg cgttcctgcg gaccgcccgg ggccatcacg ggacgatcgt
     3541 cctcggccgt gacgccggcg ccggggagtt cacggccgcc gatcggcagc tcctcgcctc
     3601 ggtcgcaggc caggcggcga tcgcgatcga gcgggccggg ctgcagcgcc agatcgtgga
     3661 gcggcgcgcc ctcgacgagg agctcgcgat cgggcggcgg atccagttgt cgctcatgcc
     3721 gcgccgcttt ccgacgatcg agggctggga gatcgccagt gcctatgagc cggcccgcga
     3781 ggtcggtggc gacttctacg acgtctttcg ccttcgcgac cgcggcgact gcgtcggcct
     3841 ggtcgtggcc gacgtcaccg gcaaggggat cccggcggcg atcctcatgg ccgacacccg
     3901 cggcctcatc cacgcggcgg cggatcacag cgacgatccc gccgagaccc tcgcccgggt
     3961 caaccggatc ctcgtcgacg agcgggcgag cgggctgttc gtgaccgtcg tccacgccgt
     4021 cctcgatcct cgcaccggac gcctcgtcct cgcccgtgcc ggacacgacc ccgtccacct
     4081 cctgcgcgcc gatggccggc tcgaggtcct cgttccgccc ggccgcctga tcgggatggt
     4141 cgccgatctc ggtgtgacct cggtcgagct gcgcgtcgaa ccgggcgacg cgttcgtcgg
     4201 ccacaccgac gggatcaccg aggcccgcgc gccggacgga tcgttctacg gcgaggaccg
     4261 gttccgggcg ctgctggccg ggctcgccgg gacgaccgcc gccgggatcg tcgcggcggt
     4321 cacagccgat gtcgcgtcgt tccggggccg cgccgagcct tccgacgacc tgaccctgct
     4381 ggttgttcgc cgccaaccgg ccgccgccgg gtaggtgatc gggcgaatcg tttcccgacg
     4441 agccgagtct gtatttcgct ccgagtcatt cgggttgacg acagtgacgg agcgatcgcg
     4501 cgatccgggc tagactcggg tggaccccca aggcccgact cgaggtcgtc ttgcccgccg
     4561 atcttctgct accgctgttc gcgctggtgc ttctcgccaa cgccgtcctt gtcgccgcag
     4621 cgatccgcgg gatgctgcgc ggtcgtccgg acctggatcg gccgacgacg atcggccgac
     4681 cgaacggcat cggcatggtt gcgtcgcgca acctcgaccg accactgacc gacgagctca
     4741 agcatgcgat cgcggcgagg caaggcatcg ccgacgaggc gccgctcgcc tcgacgcctc
     4801 cgggccaggc gccgctcgcc tcgacgcctc cggaaccggc cgcgccggac cccgaggatc
     4861 cgtccgacgc gggacccgac aacgaacgct tcagcacgtc ccaggcctcg ccggctcgcg
     4921 ccgcggcggg gtcatccgaa gcgatgccgc cggcgacgtc cccgccggcc acgtcgccgc
     4981 ccgagacacc cgccccgagg cgccggcgga cggcgcaggc cggcatcaat gccgacgcgc
     5041 gcggaccgcg tcccgcggat ccgccccgtc gcggtcggcg gcgcttctcg ttgccgcctg
     5101 aagaggatca cgagaaggtc aatcggtcca tcgaatcgtt cctgagcggc atcgatggat
     5161 ccgggtccga tcccgcggcg gggacggcgg acccctcctc cggtgggccg acgacggtcg
     5221 cgctcgtcgc cgttgacggc ctggtcgact ccggacgggc cggcctcccg acagccactg
     5281 gcgcgtcgac cgggaacgac gtcgacgatg cgcagtccgc cgatgccgtg gcgatggtcg
     5341 agcggacgct gcgtggcgcc gcacggggga gcgacgtcgt cagcgtcgac ggccgcggcc
     5401 gcttccgggt gatcctccgc ggcaccggcg agctcgcggc ccgggcctat cttcgacgga
     5461 tccggtcgac ggtcgaaccg atgctcgagg cctccgatcg gccgctccgc ttcgtggtgg
     5521 ccaccgcgac cgtcctgggc gagccgatcg aggacgccgt ccgccaggcc gatcgtcggc
     5581 tggcagcgac gctcggcgcg actcgcgacg cggagggcgc gaccggccag gaccacggtg
     5641 aaccggcctt cgacccccga gcggcgggcg actgaccgcc tagccgaccg gaccgaccgg
     5701 accgaccggc gcgccatccg gggtgccgac cggcgcgggc tcctcctcgg gttgcccccg
     5761 gtggatcgcc atgaagacga tcgcgagggc cagggaaccg atcgccacga cggcagcgat
     5821 gacctgctcc gcccggagtt gaccggcgac cacggggtca cgccaggtcg aggcaacgac
     5881 cgcccgcccg acggcccagc cggcgagggc gaacaggaag agccgcccgt cggagcggcg
     5941 gagttgcggc agcagcgacg ccaggacgat gacgaccacg acgatcgcgg tcgcgatcgc
     6001 ctcgagcgcc tgcgacggga tcgatgcgac ggccggaccc agcgagcccc acgggcccga
     6061 cccgagatac gcggttgccg gctcgccgct cgtcggcaac ccctggcccg tcccaccgag
     6121 gaccatcgcc agcttgcccg cgacgagcag gaagagcgtc gggaacgtgc aggcccgcag
     6181 ccacggtccc ggccggccgt cgaggagcag cgcgacgagc gagcccgtca gcgcgccgcc
     6241 gacgaccccg acggtcaact cgaggctgcc gacgccgggg tcgaggatcc gaccgggcac
     6301 ggccgtgaag aaggctggat ggagcaggac atagccgagg cgtccgccaa tgaccgcacc
     6361 gggcacgatg ccgaggacga cgaacaggag gtcgtcgacg cgcaggccgg acgctcgagc
     6421 ggcgactccg gcgagcacga tcgccccgaa gatcgatgcg gcgatcgcca cggtctccca
     6481 gcggacggcg ccgtccccga gatggagcag cgggtcgaag tcgaacgcga gggcggcggt
     6541 cggcacgatg ggcgtcagtg taccggcgcg acgagaccgg gggcacggcg ccgtcgatcc
     6601 gcgttcggtc gatccgttca cgacccgtgt gtcaactggc cgacacatcg gctgtcgttg
     6661 ctgcgctcac ccgggggtct cgcaggcgac cagcatctgg tcatcggtgg cgtgtcccgg
     6721 ggggagcaca gaccgaaagg gacggacgag gggttgcccg tcccgggggt agacggacag
     6781 ccgggccctg gggggaccgg cggtccgctc gacggcagcg cccggcctct ggggggaatg
     6841 ccgggcgcag ccgtcgcact cacggagggg cacatgagcg ctcattccac gctcgcgatc
     6901 cgcctcctcg gtcgcgtgga cgtccacgcc gatgacgtca cgatccggat cggcggtcgc
     6961 cacgcccagg ccctcatggc actgctggcc ctgcggccac ggccacgcct gcgcgacacg
     7021 ctcgcggccg agctctggcc cgaggcggga gcgccatcgg ccgcgtccct gcggcaggcc
     7081 ctctggctca tccgctcgag cctgacggcc gccggcgtcg atgctgatca atggctcgag
     7141 gcggaccagg acacgatcgg gctccatcgc gacgccgcgc tcgatctcga tgtcgactcc
     7201 ttcgagcggc tcgccacgag cggcgctccc gacgaccagg agcacgccct ccgggtgtat
     7261 gccggcgatc tcctcgaggg gctcggtcac gaatgcttcg ccgcggaccg cgagcggctc
     7321 tcggatctct acgaggatct cctggccgag gtggcccaga accgcctcga tcacggggac
     7381 accgtcggtg cccgccgcgc cgccagcgag ctcctcatcc gcgatcccct ccgcgaggag
     7441 gcccacgcgg tcctgatcgc ggcctacggg cgaaccggct cccggtccca ggtcgtccgc
     7501 cagtaccgtc gtgtctgcgc catcctccgg gccgagctcg cggtcgctcc cctgccggag
     7561 accgatgccg cctatcgcgt ggccctggcc gtcaccgcga cccgctcgcg cgatcgtgcc
     7621 gcggaggtcc gctacgacgc cggcccgttc gcgccggttc tcgtctcgag cgcctgagcc
     7681 tccgcccggt cacccacccg acgctcaccc gacgcccgtc caatcgccgg acctccgtct
     7741 cctccggagc ccggccgcga atcggaacgg ccccggcggt tgccggggcc gtttcgtgcg
     7801 cgagactgac ctgggtcagg gggcgacctg ggcccagtag gcgacctggt attcgtcgcc
     7861 gccaccgctc agctggatcc agccctgggc gatggtgccg ttggccaggc cggcgttgac
     7921 cgtgatcgtc agcgtcgcgc tggagttggc ggccaccgtg atcgacgacg gggtcgtgat
     7981 ccggctgtca tcgccaccga tcgtgccgcc gttgaacgcc gcgagggcgc cgccggccgg
     8041 ggtgaacttc agctctgcaa catcgaacgt gcgcgccgtc ccggtcaggt tcgtcacctt
     8101 gaccgcgatg gacgtcgcgt tcgtcttgct ggcgttgatc cggccgaagc tggcgctcgt
     8161 cggccagaac gtggcgtcgg tcgaggcggc ctcggcgagg tcctcccggc cggcgccctg
     8221 ggcctgcggc ccgacggtcg tcgacgcgtc gaacgcgttc ttgaccacga ggtccgccgt
     8281 attgacgagg gccgacttga tctgggccgg ggtccaggtc gggtgcaacc cgcggaggac
     8341 ggcggccgag cccgcgacgt gcggggccga catcgacgtg ccgctgaaga acgcccagtc
     8401 gccttcaccg ccgcagctcg caccgttggt gcaggccacg gagctgagga cgttgacccc
     8461 gaccgaggcc aggtcgggct tgatcgcgta gtcgacgttg gtcggaccct ggctgctgaa
     8521 gccggcgagg atgtcgccgt tggacgtcac gaactcggag aaggtcgcgg cgacggtgac
     8581 cgaggtcgcg tgcgccgccc ggagggctgc gccggcggtg ttcgagatca tgacggcggg
     8641 gatgtcgtcg tcgaagccga ccgacctcgc catcgccgtc ggatcaccgg cgacgttgtt
     8701 gaccacgatc acgccggtgg ctcccgccgc cttggcgttc gccaccttct ggctgaacgt
     8761 gcagacgccg cgattgatca gggccacctt gccactggcg cccgcagcga tcgcggcgca
     8821 gccgttggcg gccgtgtcga agaggccgaa cgtgccggcc ggcatcgagc cgaagtcacc
     8881 gaccgcggcg ccgacggttg cgctgctgtc gcccgtgtag gtcaggggct ggccgacgaa
     8941 gtggttgttc gtgctggcgc cgacggtgat gatgtcgcgc gcccggcccg gcgaaccgat
     9001 cgtgaacccg ccggggccct cgttgccggc cgaggcgacg acgacgacgc ccgcggcgac
     9061 ggcggtgtcg aggcccttgg ccagcaggtc gttgttgccg tggtacgcgc cgccgagcga
     9121 caggttcagg acgtccatcc cgtcgatcac ggccgcctcg accgcgttga ggatgtcctc
     9181 actgcgggcg ttgacgacgg cgccggggaa gacgttgtag ttgccgagcc aggcgccggg
     9241 cgcgatgccc gacatgtcgt cgatcgcgac gccctcgacg gtggccgtct tgccggtgac
     9301 gccggcgaca gtgcccgcgg tgtggctgcc atgatcgcct tcaccggcga ccggcagcgc
     9361 gtcgtagtcc gtctggttgt tcctgttgta gtagaccttg gcgacgatga ccttctcgct
     9421 cacgttcttg cacgtgctgc cggtcatgtc cgccgcgtcg cacttcggga agcccgccgg
     9481 ataggcgaag cccgccggat cgaagaacgg atggcggtag tcgatacccg agtcgatcat
     9541 gccgaccttg acgccggcgc cggcatccgc ccggccgccc gcggcggccc acgcggcctg
     9601 ggcgttgatg agcttgtagc tctcgctcat gtcggggtgg tagagggcgt tgtactcgac
     9661 caccgcgacg tccgtgttgt tggcgagctt gccgatcgcg gcgccgttca gcttgaccgc
     9721 gaccgcgttg agggtcacga agtactgcga ggtgatcttg gcgctcggga cattggtctg
     9781 gagccacttg gcgaaggccg agtgctcggt cttgaggtag ccgacgtact tcttggcggc
     9841 ggcgctgttc gggttgagct tggcgctcga gaccctggtc ttgaggtagc ccgggacgtg
     9901 accgtcgtac gtggcgacgg gctgctgctt gaggacgacg atcgcggacg tcttgtccag
     9961 gatcgcgttc ga
//
LOCUS       DATAIW010000523         4850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U6894 k141_148499, whole genome shotgun sequence.
ACCESSION   DATAIW010000523 DATAIW010000000
VERSION     DATAIW010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955512
            Sequence Read Archive: SRR12756227
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:09:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,059
            Genes (coding)                    :: 3,053
            CDSs (with protein)               :: 3,053
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148499"
                     /isolate="SMAG_U6894"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHM48_15405"
     CDS             complement(<1..183)
                     /locus_tag="VHM48_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756857.1"
                     /translation="MTKAVLASVESLGAFVIGGYLGPLAIIGLAILPAAAIAGVARRL
                     VRISLLLTLPIAISVVL"
     gene            complement(313..1389)
                     /locus_tag="VHM48_15410"
     CDS             complement(313..1389)
                     /locus_tag="VHM48_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756858.1"
                     /translation="MSTGWLKALVGIVVFFVVVIIGLNATGTLDATADPVTGLIASRN
                     TIIVLLGIALLLGFVAYAGTEYAQTNRLESITSQFDTRTIVLIPIAIAINIILGQTVA
                     AALKVPIYLDSIGTILVGVLAGPIAGALTGGLANLIWTYVLPAPFHSDYAAPFFIVAV
                     EIGLLAGIFGRLGFFRSRPNTSPDRLVIGAGVVVAIVALIAVYGFIPYYGKGAITFFA
                     PAVEGAPPPDGIFVFLGWAVALLLVSAVVGLIALLFVRRDLGAAYIFVAGLACGIVSA
                     IISAPISAIVFGGVTGGGADLLVAAFQKAGDSLGDSVLKQGLLQDPIDKTLTFFVVFA
                     ILGALSRRFVARFPQGEQAVGLEG"
     gene            complement(1462..2517)
                     /locus_tag="VHM48_15415"
     CDS             complement(1462..2517)
                     /locus_tag="VHM48_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2756859.1"
                     /translation="MTIVPLILDVDTGIDDSLALLYAAATPEAELVAVTCVGGNVDAH
                     QVERNTRSVLELAGRTDVEVALGRDRPLVKAVETTPDTHGPLGLGHAELPPPSRPLSE
                     RHAVDVFIDEARRRPGEITLVTCGPLTNLALAVLREPALPRLLKGWVLMGGAYRHPGN
                     TAPTTEWNIHCDPDAAKIAFTAWGVSAAAHGHPRAVALGLDVTEQARILPEHVVALAR
                     AAGSTPDDSLALARGEDPMQATRSVASNPIVRYVADALRYYMEFHARYDGFYGAFIHD
                     PLAVAAALDPSLIRTQAVAVDVELGGTLTTGETVTDWRGVWGRPPNLDVAVEADAPEF
                     LRRFVERVGRLAATRPT"
     gene            complement(2564..3391)
                     /locus_tag="VHM48_15420"
     CDS             complement(2564..3391)
                     /locus_tag="VHM48_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756860.1"
                     /translation="MRHRRNPFAPLLVALLAGSLAVVGPASPAQASSPGSGASARGAT
                     ATVGTAASKPFRIDLADRNDFVAQEDFVQCVGASMQMMRNIMGASDRTARTQSRLQAI
                     ARAASGPTRAGFVRKGASVVGWAAALNRLDSGPYRVVGADTLQEALRLAARSIRATGR
                     PVGLLVWAGRHAWVMSGFEATADPLATDGFRVTKAIVLDPLYPYGSARWGRSPKPREA
                     IAPSVLGRQFVPRRRGTWAGAPSGAAGQSLKALAGKYVIVMPYTRVLFFGRNRPVPV"
     gene            complement(3404..3928)
                     /locus_tag="VHM48_15425"
     CDS             complement(3404..3928)
                     /locus_tag="VHM48_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2756861.1"
                     /translation="MLVIGIDASAAAMAESSLRAAGPARRGGLPNALFVVAAAERPPD
                     ELSAIAAEVSILFPWGSLLRGALALGDACDAAAGIAGLVAPGGLVRVLVSIDLRDRLS
                     VPVLEATGRADLAGRWAHHGLTLTVFEPARPAEIDASGSAWWWRLATKRDRRVWRLEL
                     RRATEPSGVIAPDG"
     gene            4096..4740
                     /locus_tag="VHM48_15430"
     CDS             4096..4740
                     /locus_tag="VHM48_15430"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2756862.1"
                     /translation="MPTSQNASSRPIGSYERRQAAVRPWDPHVLDVAARVIAIIEGAR
                     PDLHVEHIGSTSVPGLPGKGIVDLGTDADPADIPTITAAMYELGFGPQPGPDPWPATR
                     PMHVGSVVDDGIEYRVHFHVHPRGTGDLDKDLRFRDALRADPALVEGYARIKQEIVDR
                     AGGPLDGVIYQAEKGGWILDVYDRLGIERPANVGGGPTGEADQGVIEPERGGST"
BASE COUNT          804 a         1649 c         1771 g          626 t
ORIGIN      
        1 gaggacgact gagatcgcga tcggcagggt caggaggagc gagatccgga cgaggcgccg
       61 ggcgacgccg gcaatcgcgg cggccggcag gatggccagg ccgatgatcg ccagtggtcc
      121 gagatagccg ccgatcacga acgcaccgag ggattcgacg gacgcgagca cggcctttgt
      181 caacgggttc aggcggtgcc agggcgatgg cgccgccggg gcgaggcctg cgaggaaggc
      241 agattcggat ggcgcggatc cggcgtcgtc gatcggccgg tcgacgaggt cgttgtctgc
      301 cgggacgatc ggctagccct ccagcccgac ggcctgctcg ccctgcggga agcgggcaac
      361 gaaccgacgg ctgagtgcac cgaggatcgc gaacacgacg aagaaggtca gcgtcttgtc
      421 gatcggatcc tggaggaggc cctgtttgag gactgaatcg ccgaggctgt cgccggcctt
      481 ctggaaggcg gccacgagga ggtccgcccc gccacctgtg acgccgccga acacgatcgc
      541 ggagatcggg gcggagatga tcgccgacac gatcccgcat gccaggccgg ccacgaagat
      601 gtaagcggcg ccgagatcac gccggacgaa caggagggcg atgaggccga cgactgcgct
      661 gacgagcagc agggcaaccg cccagcccag gaacacgaag atcccgtcgg gcggtggggc
      721 gccctcgacc gccggggcaa agaacgtgat cgcacccttg ccgtagtacg gaatgaagcc
      781 gtagaccgcg atcagagcga cgatcgcgac cacgacaccc gcaccgatca ccagtcggtc
      841 cggcgacgtg ttggggcggc tccggaagaa gccgagccgg ccgaagatcc cggccagcag
      901 cccgatctcg acggcgacga tgaagaacgg cgcggcgtag tcggaatgga acggcgccgg
      961 caggacgtag gtccagatga ggttggcgag gccgccggtc agggcgccgg cgatcggccc
     1021 ggccaggacg ccgacaagga tcgtcccgat cgagtcgaga tagatcggca ccttcagcgc
     1081 ggccgcgacc gtctgtccga ggatgatgtt gatcgcgatc gcgatcggga tcaggacgat
     1141 cgtccgcgtg tcgaactggc tggtgatcga ttcgagccgg ttcgtctggg cgtattccgt
     1201 tccggcgtag gcgacgaagc cgaggaggag cgcgatcccg agcaggacga tgatcgtgtt
     1261 gcgggatgcg atcagaccgg tgaccggatc ggccgtcgcg tcgagcgtgc ccgtcgcgtt
     1321 cagtccgatg atcacgacca cgaagaacac gacgatcccg acgagggcct tcagccagcc
     1381 ggtcgacatg agacctcctc cgttgccgcg caaggaccgc cgcacgccaa cggtccatgc
     1441 ctaccctccg cccgtagtgt gctacgtcgg acgggtcgcc gccagtctcc cgacgcgttc
     1501 cacgaaccgt cgcaggaact ccggcgcgtc ggcctcgacc gccacatcga ggttcggcgg
     1561 ccggccccag acgccgcgcc aatcggtcac ggtctcgccg gtcgtcaacg tcccgccgag
     1621 ctccacgtcg accgcgaccg cctgggtccg gatcagcgac gggtcgagag ccgcggccac
     1681 cgccagcggg tcgtggatga acgccccgta gaagccgtcg taccgggcgt ggaactccat
     1741 gtagtaccgc agcgcatcgg cgacgtagcg gacgatcggg ttgctcgcga ccgagcgcgt
     1801 cgcctgcatc gggtcctcgc cgcgagccag ggccagcgaa tcgtcgggcg tgctgccggc
     1861 ggcgcgggcg agggcgacga cgtgctccgg caggatcctt gcctgctccg tgacgtcgag
     1921 gccgagcgcg accgcccgcg gatgaccgtg ggccgcagct gatacgcccc atgcggtgaa
     1981 cgcgatcttc gccgcgtccg ggtcgcagtg gatgttccac tcggtcgtcg gcgccgtgtt
     2041 gccgggatga cgataggcgc cacccatgag aacccagccc ttgagcagcc gcggcagggc
     2101 cggctcgcgg aggacggcga gggcgaggtt tgtcagcggt ccgcaggtca ccagcgtgat
     2161 ctcgcccggc cgtcgacggg cctcgtcgat gaagacatcg accgcgtggc gctcgctgag
     2221 agggcggctc ggtggaggga gctcggcgtg accgagaccc agcggcccgt gcgtgtcggg
     2281 cgtcgtctcg accgccttca cgagcggccg atcacggccg agagcgacct cgacgtcggt
     2341 ccggccggca agctcgagga ccgacctggt gttgcgctcg acctggtggg cgtcgacgtt
     2401 gccgccgacg caggtcaccg cgaccagctc ggcctcgggc gtggccgcgg cgtagagaag
     2461 ggcgagcgaa tcgtcgatcc cggtgtcgac gtcgaggatc aggggaacga tcgtcatctt
     2521 cggaaggtag cgcaccatcc ggcggcgggc gcgggctggg ccgtcagaca gggacgggcc
     2581 gattgcgccc gaagaagagg acccgcgtat agggcatgac gatgacgtac ttcccggcga
     2641 gagccttcag cgactggccg gcagccccgc tcggagcgcc cgcccacgtc ccccggcgtc
     2701 gcggcacgaa ctgcctgccc aggaccgacg gcgcgatcgc ttcgcgcggc ttcggactgc
     2761 gtccccagcg cgccgagccg taggggtaga gcgggtcgag gacgatcgcc ttggtgaccc
     2821 ggaacccgtc ggtggcgagt ggatccgccg tcgcctcgaa gccgctcatc acccaggcat
     2881 gacgcccggc ccagacgagg agcccgaccg gccggccggt tgcgcggatc gatcgagccg
     2941 cgagtcgaag cgcctcctgg agcgtgtccg cgccgacgac gcgatagggc cccgaatcga
     3001 gacggttcaa cgcggcggcc cagccgacga cgctggcacc cttgcgcacg aatccagcgc
     3061 gggtcggacc ggacgcggcg cgggcgatcg cctggagccg tgactgggtc cgggcggttc
     3121 gatcgctcgc gcccatgatg ttgcgcatca tctgcatgct ggccccaacg cactggacga
     3181 agtcttcctg ggccacgaag tcgttacggt cggcaaggtc gatccggaac ggcttcgagg
     3241 ctgcggtccc aaccgtggcc gtggctccgc gcgcgctcgc cccgctcccc gggctggacg
     3301 cctgggcggg ggaagccggt ccgaccacgg ccagcgagcc ggcaagaagg gcgaccagca
     3361 gcggcgcgaa cgggttccgg cggtggcgca tgatcgccgc attctagccg tcgggagcaa
     3421 tgacgccgga cggctccgtt gcccggcgca gctcgagacg ccagacccgc cgatcgcgct
     3481 tcgtcgcgag gcgccaccac caggctgagc ccgaggcgtc gatctccgcg ggccgggccg
     3541 gctcgaagac cgtgagcgtc agcccgtgat gggcccaccg gccggccaga tcggcccgcc
     3601 cggtcgcctc gaggaccggc acggacaggc ggtcgcgcag atcgatcgag acgagcaccc
     3661 ggaccagtcc gcccggggcg acgaggccgg cgatgcccgc cgcggcgtcg cacgcatcgc
     3721 cgagggccag ggcgccgcgg agcagagagc cccacggaaa gaggatcgat acctcggccg
     3781 cgatcgcgct cagctcgtcg ggcggacgct cggcggccgc gacgacgaac agggcgttcg
     3841 gcagtccgcc cctgcgcgcg ggaccggcag cacgcaacga cgattcggcc attgcggcgg
     3901 cggacgcgtc gataccgatc acgagcacgg ccaactcccg tcgcgcacgg gcgaggaccg
     3961 ctcgaccgtc gccggttccg aggtcgatga cgacccgatc ggcgcgagcg atgaagggtt
     4021 cgagttagcc gacatccacc tcgtggatgc gccggccgac gacgcgtcgc atggccggga
     4081 atctacactg ccccggtgcc aacgtcgcag aacgcgtcgt cgcgtccgat cggatcctac
     4141 gagcggcgtc aggccgccgt ccggccgtgg gatccgcacg tcctcgacgt agcggcgcgc
     4201 gtgatcgcga tcatcgaggg cgcccggccc gacctccatg tcgagcatat cggcagcacg
     4261 tccgttccgg ggctgccggg caagggaatc gtggatctcg gaacggatgc ggacccggcc
     4321 gacatcccga cgatcacggc cgcgatgtac gagctcgggt tcgggccgca gcccggtccc
     4381 gatccgtggc cggcgacccg gccgatgcat gtcgggtcgg tggtcgacga tggcatcgag
     4441 taccgggttc acttccacgt ccatccacgc gggaccggcg atctcgacaa ggatctccgc
     4501 tttcgtgacg ccctccgtgc cgacccggcg ctcgtcgagg gctacgcccg gatcaaacag
     4561 gagatcgtcg accgggcggg cggcccgctc gacggcgtca tctaccaggc ggagaagggc
     4621 ggctggatcc tcgacgtcta cgatcgactc gggatcgagc ggccggccaa cgtcggcggc
     4681 ggccccaccg gcgaggccga ccaaggggtg attgagcccg agcgaggagg atcgacatga
     4741 ccgacggttc cggaagcccg accgagcgac gcgtcgggat cgtcgtcggc agccggtccg
     4801 acatcgcggc cgccgagcgg gcgatggcgg tcctcgacga gctcgaggtc
//
